title: Head and stalk structures of soybean vacuolar membranes.
abstract: Highly purified tonoplast fractions isolated by preparative free-flow electrophoresis from hypocotyls of etiolated soybean (Glycine max L. (Merr.)) were examined by negative-staining electron microscopy, and many but not all vesicles were found to exhibit head and stalk structures resembling the 9-nm stalked F1 ATPase particles reported previously for Neurospora (Bowman et al., 1989, J. Biol. Chem. 264, 15606-15612). The structures show distinguishing characteristics similar to those for Neurospora. These include a cleft in the particle not exhibited by mitochondrial F1 ATPase and a tendency to disappear from the membrane when treated with nitrate plus Mg(-2+)-ATP-containing solutions. The position of the stalked ATPase structures, indicates that some of the tonoplast vesicles were oriented cytoplasmic side out whereas others were oriented cytoplasmic side in.
authors.affiliation: Department of Medicinal Chemistry, Purdue University, 47907, West Lafayette, IN, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Morré
authors.forename: C
authors.initials: C
authors.lastname: Liedtke
authors.forename: A O
authors.initials: AO
authors.lastname: Brightman
authors.forename: G F
authors.initials: GF
authors.lastname: Scherer