title: Molecular cloning and expression analysis of mannose receptor C type 1 in grass carp (Ctenopharyngodon idella).
abstract: Mannose receptor C type 1 (MRC1) is a pattern-recognition receptor (PRR) which plays a significant role in immune responses. Much work on MRC1 has been done in mammals and birds while little in fish. In this study, we cloned and characterized MRC1 in grass carp (gcMR). The full-length gcMR contained 5291bp encoding a putative protein of 1432 amino acids. The predicted amino acid sequences showed that gcMR contained a signal peptide, a cysteine-rich (CR) domain, a fibronectin type II (FN II) domain, eight C-type lectin-like domains (CTLDs), a transmembrane domain and a short cytoplasmic domain. gcMR were constitutively expressed in different organs with the higher expression in spleen and head kidney. During embryonic development, gcMR transcript levels were highest at cleavage stage. The up-regulation expression of gcMR, IL-1β and TNF-α in liver, spleen, head kidney and intestine after Aeromonas hydrophila infection indicating it involved in innate immune regulation during bacterial infections.
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Lichun
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Xiaoheng
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Xi
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Shun
authors.initials: S
authors.lastname: Wei
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Fang
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Nan
authors.initials: N
authors.lastname: Chen
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Zemao
authors.initials: Z
authors.lastname: Gu
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Xueqin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan 430070, People's Republic of China; Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan 430070, People's Republic of China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Asim
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: liuxl@mail.hzau.edu.cn.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Hubei Collaborative Innovation Center for Freshwater Aquaculture, Wuhan 430070, People's Republic of China; Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan 430070, People's Republic of China; State Key Laboratory of Agricultural Microbiology, Wuhan 430070, People's Republic of China. Electronic address: linli@mail.hzau.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D002347:Carps / Q000276:immunology*; D003001:Cloning, Molecular; D047108:Embryonic Development; D020869:Gene Expression Profiling; D016905:Gram-Negative Bacterial Infections / Q000276:immunology*; D007113:Immunity, Innate; D007375:Interleukin-1 / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D037181:Lectins, C-Type / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary / Q000235:genetics; D051192:Receptors, Pattern Recognition / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D007375:Interleukin-1; D037181:Lectins, C-Type; D051192:Receptors, Pattern Recognition; D014409:Tumor Necrosis Factor-alpha
keywords: Aeromonas hydrophila; Expression; Grass carp (Ctenopharyngodon idella); Mannose receptor C type 1 (MRC1)