title: Obese locus in WNIN/obese rat maps on chromosome 5 upstream of leptin receptor.
abstract: WNIN/Obese (WNIN/Ob) rat a new mutant model of metabolic syndrome was identified in 1996 from an inbred Wistar rat strain, WNIN. So far several papers are published on this model highlighting its physical, biochemical and metabolic traits. WNIN/Ob is leptin resistant with unaltered leptin or its receptor coding sequences--the two well-known candidate genes for obesity. Genotyping analysis of F2 progeny (raised from WNIN/Ob × Fisher--344) in the present study localized the mutation to a recombinant region of 14.15cM on chromosome 5. This was further corroborated by QTL analysis for body weight, which narrowed this region to 4.43 cM with flanking markers D5Rat256 & D5Wox37. Interval mapping of body weight QTL shows that the LOD score peak maps upstream of leptin receptor and shows an additive effect suggesting this as a novel mutation and signifying the model as a valuable resource for studies on obesity and metabolic syndrome.
authors.affiliation: Molecular Genetics, National Centre for Laboratory Animal Science (NCLAS), National Institute of Nutrition (NIN), Hyderabad, India.
authors.forename: Rajender Rao
authors.initials: RR
authors.lastname: Kalashikam
authors.forename: Kiran Kumar
authors.initials: KK
authors.lastname: Battula
authors.forename: Veerababu
authors.initials: V
authors.lastname: Kirlampalli
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Friedman
authors.forename: Giridharan
authors.initials: G
authors.lastname: Nappanveettil
mesh_terms: D000818:Animals; D001835:Body Weight / Q000235:genetics; D033481:Chromosomes, Mammalian / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D024821:Metabolic Syndrome / Q000235:genetics; D009154:Mutation / Q000235:genetics; D009765:Obesity / Q000235:genetics*; D051381:Rats; D011916:Rats, Inbred F344; D054411:Receptors, Leptin / Q000235:genetics*
chemical_list: D054411:Receptors, Leptin