title: Detection of Hg2+ based on the selective inhibition of peroxidase mimetic activity of BSA-Au clusters.
abstract: It was found that Hg(2+) can inhibit the peroxidase mimetic activity of bovine serum albumin (BSA) protected Au clusters (BSA-Au) due to the specific interaction between Hg(2+) and Au(+) existed onto the surface of BSA-Au clusters. By coupling with 3, 3', 5, 5'-tetramethylbenzidine (TMB)-H2O2 chromogenic reaction, a novel method for Hg(2+) detection was developed based on the inhibiting effect of Hg(2+) on BSA-Au clusters peroxidase-like activity. This method exhibited high selectivity and sensitivity. As low as 3 nM (0.6 ppb, 3σ) Hg(2+) could be detected with a linear range from 10 nM (2 ppb) to 10 µM (2 ppm) and this method was successfully applied for the determination of total mercury content in skin lightening products.
authors.affiliation: The Key Laboratory of Luminescence and Real-time Analysis (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xi-Liang
authors.initials: XL
authors.lastname: Wang
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Liang
authors.forename: Yi-Juan
authors.initials: YJ
authors.lastname: Long
authors.forename: Qin-Long
authors.initials: QL
authors.lastname: Wang
authors.forename: Hai-Jie
authors.initials: HJ
authors.lastname: Zhang
authors.forename: Xiao-Xiao
authors.initials: XX
authors.lastname: Huang
authors.forename: Hu-Zhi
authors.initials: HZ
authors.lastname: Zheng
mesh_terms: D001560:Benzidines / Q000737:chemistry; D040761:Biomimetic Materials; D004340:Drug Contamination; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D008628:Mercury / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000737:chemistry; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D062327:Skin Lightening Preparations / Q000737:chemistry*
chemical_list: D001560:Benzidines; D062327:Skin Lightening Preparations; D012710:Serum Albumin, Bovine; C021758:3,3',5,5'-tetramethylbenzidine; D006046:Gold; D006861:Hydrogen Peroxide; D009195:Peroxidase; D008628:Mercury
keywords: BSA-Au clusters; Detection; Hg(2+); Peroxidase-like activity