title: Bi-domain protein tyrosine phosphatases reveal an evolutionary adaptation to optimize signal transduction.
abstract: CONCLUSIONS
  The bi-domain protein tyrosine phosphatases (PTPs) exemplify functional evolution in signaling proteins for optimal spatiotemporal signal transduction. Bi-domain PTPs are products of gene duplication. The catalytic activity, however, is often localized to one PTP domain. The inactive PTP domain adopts multiple functional roles. These include modulation of catalytic activity, substrate specificity, and stability of the bi-domain enzyme. In some cases, the inactive PTP domain is a receptor for redox stimuli. Since multiple bi-domain PTPs are concurrently active in related cellular pathways, a stringent regulatory mechanism and selective cross-talk is essential to ensure fidelity in signal transduction.
  
  
  BACKGROUND
  The inactive PTP domain is an activator for the catalytic PTP domain in some cases, whereas it reduces catalytic activity in other bi-domain PTPs. The relative orientation of the two domains provides a conformational rationale for this regulatory mechanism. Recent structural and biochemical data reveal that these PTP domains participate in substrate recruitment. The inactive PTP domain has also been demonstrated to undergo substantial conformational rearrangement and oligomerization under oxidative stress.
  
  
  CONCLUSIONS
  The role of the inactive PTP domain in coupling environmental stimuli with catalytic activity needs to be further examined. Another aspect that merits attention is the role of this domain in substrate recruitment. These aspects have been poorly characterized in vivo. These lacunae currently restrict our understanding of neo-functionalization of the inactive PTP domain in the bi-domain enzyme. It appears likely that more data from these research themes could form the basis for understanding the fidelity in intracellular signal transduction.
authors.affiliation: 1 Molecular Biophysics Unit, Indian Institute of Science , Bangalore, India .
authors.forename: Lalima Gagan
authors.initials: LG
authors.lastname: Ahuja
authors.forename: Balasubramanian
authors.initials: B
authors.lastname: Gopal
mesh_terms: D019143:Evolution, Molecular; D006801:Humans; D008958:Models, Molecular; D017434:Protein Structure, Tertiary; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry* / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D017027:Protein Tyrosine Phosphatases