title: "Jump start and gain" model for dosage compensation in Drosophila based on direct sequencing of nascent transcripts.
abstract: Dosage compensation in Drosophila is mediated by the MSL complex, which increases male X-linked gene expression approximately 2-fold. The MSL complex preferentially binds the bodies of active genes on the male X, depositing H4K16ac with a 3' bias. Two models have been proposed for the influence of the MSL complex on transcription: one based on promoter recruitment of RNA polymerase II (Pol II), and a second featuring enhanced transcriptional elongation. Here, we utilize nascent RNA sequencing to document dosage compensation during transcriptional elongation. We also compare X and autosomes from published data on paused and elongating polymerase in order to assess the role of Pol II recruitment. Our results support a model for differentially regulated elongation, starting with release from 5' pausing and increasing through X-linked gene bodies. Our results highlight facilitated transcriptional elongation as a key mechanism for the coordinated regulation of a diverse set of genes.
authors.affiliation: Center for Biomedical Informatics, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Francesco
authors.initials: F
authors.lastname: Ferrari
authors.forename: Annette
authors.initials: A
authors.lastname: Plachetka
authors.forename: Artyom A
authors.initials: AA
authors.lastname: Alekseyenko
authors.forename: Youngsook L
authors.initials: YL
authors.lastname: Jung
authors.forename: Fatih
authors.initials: F
authors.lastname: Ozsolak
authors.forename: Peter V
authors.initials: PV
authors.lastname: Kharchenko
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Park
authors.forename: Mitzi I
authors.initials: MI
authors.lastname: Kuroda
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004303:Dosage Compensation, Genetic; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D005260:Female; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014960:X Chromosome
chemical_list: D029721:Drosophila Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors