title: New relationships of human hematopoietic lineages facilitate detection of multipotent hematopoietic stem and progenitor cells.
abstract: Three important goals of hematopoietic stem cell research are to understand of how hematopoietic stem cells (HSCs) self-renew, how lineage commitment takes place, and how HSCs can be expanded ex vivo. Research in this area requires a reliable model of hematopoiesis. Performing detailed functional analyses of human hematopoietic progenitor subsets, we recently gained evidence for new hematopoietic lineage relationships. (1) According to our data, neutrophils belong to the same branch of the hematopoietic tree as lymphocytes. In contrast, eosinophils and basophils derive from another branch, the erythro-myeloid branch. Here, after introducing the newly proposed hematopoietic model, we discuss its consequences for the identification and expansion of human multipotent progenitors and suggest a fast and reliable method to screen for multipotent hematopoietic cells in vitro.
authors.affiliation: Institute for Transfusion Medicine; University Hospital Essen; University of Duisburg-Essen; Essen, Germany.
authors.forename: André
authors.initials: A
authors.lastname: Görgens
authors.forename: Stefan
authors.initials: S
authors.lastname: Radtke
authors.forename: Peter A
authors.initials: PA
authors.lastname: Horn
authors.forename: Bernd
authors.initials: B
authors.lastname: Giebel
mesh_terms: D000818:Animals; D019070:Cell Lineage; D006410:Hematopoiesis / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D039902:Multipotent Stem Cells / Q000502:physiology*
keywords: CD133; EMPs; GMPs; LMPPs; MEPs; MPPs; hematopoietic stem cells; human hematopoiesis; progenitors