title: Phenylalanine ammonia-lyase activity in suspension cultures of Ulmus pumila and U. campestris treated with spores of Ceratocystis ulmi.
abstract: Cell suspension cultures of a Ceratocystis ulmi-resistant (Ulmus pumila) and a -susceptible elm (U.campestris) were established from leaf callus tissue. Treatment of cultures with spores of C.ulmi induced a large increase in the activity of phenylalanine ammonialyase, only in the cells of the resistant species U.pumila with a maximum after 24 h. Inoculated U.pumila cells also excreted a red unidentified chemical into the culture medium. Neither responses were induced in inoculated U.campestris cultures. The results are discussed in relation to the development of the elm cell culture system as a model for studying the differential biochemical mechanisms of disease resistance in elms.
authors.affiliation: Departamento de Biología Vegetal, Facultad de Farmacia, Universidad de Salamanca, Spain.
authors.forename: M P
authors.initials: MP
authors.lastname: Corchete
authors.forename: J J
authors.initials: JJ
authors.lastname: Diez
authors.forename: T
authors.initials: T
authors.lastname: Valle