title: Early stationary phase culture supernatant accelerates growth of sputum cultures collected after initiation of anti-tuberculosis treatment.
abstract: We investigated the effect of Mycobacterium tuberculosis culture supernatant added to sputum cultures collected during the first 8 weeks of anti-tuberculosis treatment. With ongoing treatment duration, time to culture positivity decreased significantly in supernatant-enriched cultures, possibly due to stimulation of dormant or slowly metabolizing M. tuberculosis cells.
authors.affiliation: Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, the Netherlands; Division of Medical Physiology, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.
authors.forename: E
authors.initials: E
authors.lastname: Kolwijck
authors.forename: S O
authors.initials: SO
authors.lastname: Friedrich
authors.forename: M N
authors.initials: MN
authors.lastname: Karinja
authors.forename: J
authors.initials: J
authors.lastname: van Ingen
authors.forename: R M
authors.initials: RM
authors.lastname: Warren
authors.forename: A H
authors.initials: AH
authors.lastname: Diacon
mesh_terms: D000995:Antitubercular Agents / Q000627:therapeutic use*; D003470:Culture Media / Q000737:chemistry; D006133:Growth Substances / Q000378:metabolism*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000254:growth & development* / Q000302:isolation & purification* / Q000378:metabolism; D013183:Sputum / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy* / Q000382:microbiology*
chemical_list: D000995:Antitubercular Agents; D003470:Culture Media; D006133:Growth Substances
keywords: Dormancy; Mycobacterium tuberculosis; resuscitation promoting factors; sputum; treatment