title: Crystallization and preliminary X-ray diffraction of the RNA demethylase ALKBH5.
abstract: N(6)-methyladenosine (m6A) is a ubiquitous modification found in mammalian mRNA and long noncoding RNA. ALKBH5 is a member of the iron(II)- and 2-oxoglutarate-dependent AlkB oxygenase family and has been shown to catalyze the oxidative demethylation of N(6)-methyladenosine in RNA. The ALKBH5 protein was purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted to 2.4 Å resolution using synchrotron radiation. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 57.456, b = 83.406, c = 92.909 Å, α = β = γ = 90.00° and one molecule in the asymmetric unit.
authors.affiliation: College of Biological Sciences, China Agricultural University, Haidian District, Beijing 100094, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Zhifu
authors.initials: Z
authors.lastname: Han
mesh_terms: D000071502:AlkB Homolog 5, RNA Demethylase; D002850:Chromatography, Gel; D003460:Crystallization; D049308:Dioxygenases / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D014961:X-Ray Diffraction
chemical_list: D008565:Membrane Proteins; D049308:Dioxygenases; C559920:ALKBH5 protein, human; D000071502:AlkB Homolog 5, RNA Demethylase
keywords: ALKBH5; N6-methyladenosine