title: Identification of a novel flavonoid glycoside sulfotransferase in Arabidopsis thaliana.
abstract: The discovery of sulfated flavonoids in plants suggests that sulfation may play a regulatory role in the physiological functions of flavonoids. Sulfation of flavonoids is mediated by cytosolic sulfotransferases (SULTs), which utilize 3'-phosphoadenosine 5'-phosphosulfate (PAPS) as the sulfate donor. A novel SULT from Arabidopsis thaliana, designated AtSULT202B7 (AGI code: At1g13420), was cloned and expressed in Escherichia coli. Using various compounds as potential substrates, we demonstrated, for the first time, that AtSULT202B7 displayed sulfating activity specific for flavonoids. Intriguingly, the recombinant enzyme preferred flavonoid glycosides (e.g. kaempferol-3-glucoside and quercetin-3-glucoside) rather than their aglycone counterparts. Among a series of hydroxyflavones tested, AtSULT202B7 showed the enzymatic activity only for 7-hydroxyflavone. pH-dependency study showed that the optimum pH was relatively low (pH 5.5) compared with those (pH 6.0-8.5) previously reported for other isoforms. Based on the comparison of high performance (pressure) liquid chromatography (HPLC) retention times between sulfated kaempferol and the deglycosylated product of sulfated kaempferol-3-glucoside, the sulfation site in sulfated kaempferol-3-glucoside appeared to be the hydroxyl group of the flavonoid skeleton. In addition, by using direct infusion mass spectrometry, it was found that the sulfated product had one sulfonate group within the molecule. These results indicated that AtSULT202B7 functions as a flavonoid glycoside 7-sulfotransferase.
authors.affiliation: Department of Biochemistry and Applied Biosciences; Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki, Miyazaki 889-2192, Japan; and Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, The University of Toledo, OH 43614, USA.
authors.forename: Takuyu
authors.initials: T
authors.lastname: Hashiguchi
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Sakakibara
authors.forename: Takehiko
authors.initials: T
authors.lastname: Shimohira
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Kurogi
authors.forename: Masao
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nishiyama
authors.forename: Ryo
authors.initials: R
authors.lastname: Akashi
authors.forename: Ming-Cheh
authors.initials: MC
authors.lastname: Liu
authors.forename: Masahito
authors.initials: M
authors.lastname: Suiko
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology*; D015239:Arylsulfotransferase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D044949:Kaempferols / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D044949:Kaempferols; D009005:Monosaccharides; C511963:kaempferol-3-O-glucoside; D015239:Arylsulfotransferase
keywords: Arabidopsis thaliana; AtSULT202B7; flavonoid glycoside; sulfation; sulfotransferase