title: Resveratrol protects mouse oocytes from methylglyoxal-induced oxidative damage.
abstract: Methylglyoxal, a reactive dicarbonyl compound, is mainly formed from glycolysis. Methylglyoxal can lead to the dysfunction of mitochondria, the depletion of cellular anti-oxidation enzymes and the formation of advanced glycation ends. Previous studies showed that the accumulation of methylglyoxal and advanced glycation ends can impair the oocyte maturation and reduce the oocyte quality in aged and diabetic females. In this study, we showed that resveratrol, a kind of phytoalexin found in the skin of grapes, red wine and other botanical extracts, can alleviate the adverse effects caused by methylglyoxal, such as inhibition of oocyte maturation and disruption of spindle assembly. Besides, methylglyoxal-treated oocytes displayed more DNA double strands breaks and this can also be decreased by treatment of resveratrol. Further investigation of these processes revealed that methylglyoxal may affect the oocyte quality by resulting in excessive reactive oxygen species production, aberrant mitochondrial distribution and high level lipid peroxidation, and resveratrol can block these cytotoxic changes. Collectively, our results showed that resveratrol can protect the oocytes from methylglyoxal-induced cytotoxicity and this was mainly through the correction of the abnormity of cellular reactive oxygen species metabolism.
authors.affiliation: Organ Transplantation Institute, Medical College, Xiamen University, Xiamen City, Fujian Province, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: He
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.forename: Lu
authors.initials: L
authors.lastname: Ding
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Shen
authors.forename: Fei
authors.initials: F
authors.lastname: Zhang
authors.forename: Mao-Bi
authors.initials: MB
authors.lastname: Zhu
authors.forename: Bai-Hui
authors.initials: BH
authors.lastname: Xu
authors.forename: Zhong-Quan
authors.initials: ZQ
authors.lastname: Qi
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Wang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D053903:DNA Breaks, Double-Stranded / Q000187:drug effects; D005260:Female; D005455:Fluorescent Antibody Technique; D017127:Glycation End Products, Advanced / Q000009:adverse effects* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D018613:Microscopy, Confocal; D009865:Oocytes / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D011765:Pyruvaldehyde / Q000009:adverse effects* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D017127:Glycation End Products, Advanced; D017382:Reactive Oxygen Species; D013267:Stilbenes; D011765:Pyruvaldehyde; D000077185:Resveratrol