title: Purification and translation of ovalbumin, conalbumin, ovomucoid and lysozyme messenger RNA.
abstract: Messenger RNAs for 4 egg white proteins (ovalbumin, conalbumin, ovomucoid and lysozyme) were assayed in a cell-free, protein-synthesizing system derived from rabbit reticulocytes. Each of these messengers was purified about 33-fold from hen oviduct polysomal RNA using oligo(dT) cellulose. The apparent minimal translational efficiencies varied from 22 translations for each ovalbumin mRNA to less than 1 for lysozyme mRNA. These messengers are not polycistronic with each other since they have distinct sedimentation values of: lysozyme, 8.5S; ovomucoid, 11S; ovalbumin, 15S; and conalbumin 18S. Several aspects of this system indicate that it will be valuable for dissecting the fine control of mRNA metabolism.
authors.affiliation: Dept. of Biochemistry, Searle Research Laboratories, High Wycombe, Bucks., England.
authors.forename: R D
authors.initials: RD
authors.lastname: Palmiter
authors.forename: L T
authors.initials: LT
authors.lastname: Smith