title: Determining the concentrations of the squamous cell carcinoma antigen using the Maglumi 2000 automatic analyzer.
abstract: OBJECTIVE
  The aim of this paper was to validate the use of the chemiluminescence immunoassay (CLIA) for detecting the squamous cell carcinoma antigen (SCCa) in human serum or plasma using a Maglumi 2000 automatic analyzer.
  
  
  METHODS
  We performed a pilot study to examine both the sensitivity and specificity of the SCCa, in diagnosing squamous cell carcinomas. High levels of SCCa are helpful in diagnosing lung cancer, especially squamous cell carcinoma.
  
  
  RESULTS
  The method was linear to 22.81 ng/mL SCCa, with a detection limit of 0.02 ng/mL. An entire assay can be completed in 40 mins. The coefficients of variation (CV) of the intra- and inter-assays were less than 5% and 6%, respectively. There was a good correlation between the present and manual method. The SCCa discriminated between the squamous cell carcinoma patients and healthy controls with an area under the receiver operating characteristic (ROC) curve of 0.9231.
  
  
  CONCLUSIONS
  These results indicate that the SCCa assay showed good performance using a Maglumi 2000 automatic analyzer. This new and simple analytic system will promote the application of SCCa in clinical laboratories.
authors.affiliation: Shenzhen Second People's hospital, (The First Affiliated Hospital of Shenzhen University), Shenzhen, Guangdong, P.R. China - qisuwen2011@yahoo.cn.
authors.forename: X
authors.initials: X
authors.lastname: Ni
authors.forename: S
authors.initials: S
authors.lastname: Qi
mesh_terms: D000951:Antigens, Neoplasm / Q000097:blood*; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000276:immunology; D016022:Case-Control Studies; D004938:Esophageal Neoplasms / Q000097:blood; D005260:Female; D006801:Humans; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods; D008175:Lung Neoplasms / Q000097:blood; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000097:blood; D012372:ROC Curve; D015843:Serpins / Q000097:blood*; D002583:Uterine Cervical Neoplasms / Q000097:blood
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D015843:Serpins; C051072:squamous cell carcinoma-related antigen