title: General method for regulating protein stability with light.
abstract: Post-translational regulation of protein abundance in cells is a powerful tool for studying protein function. Here, we describe a novel genetically encoded protein domain that is degraded upon exposure to nontoxic blue light. We demonstrate that fusion proteins containing this domain are rapidly degraded in cultured cells and in zebrafish upon illumination.
authors.affiliation: Department of Chemical & Systems Biology, Stanford University , Stanford California, 94305, USA.
authors.forename: Kimberly M
authors.initials: KM
authors.lastname: Bonger
authors.forename: Rishi
authors.initials: R
authors.lastname: Rakhit
authors.forename: Alexander Y
authors.initials: AY
authors.lastname: Payumo
authors.forename: James K
authors.initials: JK
authors.lastname: Chen
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Wandless
mesh_terms: D000818:Animals; D018554:Avena / Q000737:chemistry / Q000235:genetics*; D008027:Light; D008164:Luminescent Proteins / Q000737:chemistry* / Q000235:genetics*; D051379:Mice; D041681:NIH 3T3 Cells; D010782:Photolysis; D056949:Phototropins / Q000737:chemistry* / Q000235:genetics*; D015202:Protein Engineering; D055550:Protein Stability; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics; D015027:Zebrafish
chemical_list: D008164:Luminescent Proteins; D056949:Phototropins; D011993:Recombinant Fusion Proteins