title: Photothermal ablation of tumor cells using a single-walled carbon nanotube-peptide composite.
abstract: Single-walled carbon nanotubes (SWCNTs) are known to have great potential for biomedical applications such as photothermal ablation of tumor cells in combination with near-infrared (NIR) irradiation. In this study, the photothermal activity of a novel SWCNTs composite with a designed peptide having a repeated structure of H-(-Lys-Phe-Lys-Ala-)7-OH [(KFKA)7] against tumor cells was evaluated in vitro and in vivo. The SWCNT-(KFKA)7 composite demonstrated high aqueous dispersibility that enabled SWCNTs to be used in tumor ablation. The NIR irradiation of SWCNT-(KFKA)7 solution resulted in a rapid temperature increase dependent on the SWCNTs concentration up to 50μg/ml. Three minutes of NIR irradiation of a colon 26 or HepG2 cell culture incubated with SWCNT-(KFKA)7 resulted in remarkable cell damage, while that by single treatment with SWCNT-(KFKA)7 or NIR irradiation alone was moderate. The intratumoral injection of SWCNT-(KFKA)7 solution followed by NIR irradiation resulted in a rapid increase of the temperature to 43°C in the subcutaneously inoculated colon 26 tumor based on thermographic observation and remarkable suppression of tumor growth compared with treatment with only SWCNT-(KFKA)7 injection alone or NIR irradiation alone. These results suggest the a great potential of an SWCNT-peptide composite for use in photothermal cancer therapy.
authors.affiliation: Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Kyoto, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Hashida
authors.affiliation: Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
authors.forename: Hironori
authors.initials: H
authors.lastname: Tanaka
authors.affiliation: Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
authors.forename: Shuwen
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kawakami
authors.affiliation: Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
authors.forename: Fumiyoshi
authors.initials: F
authors.lastname: Yamashita
authors.affiliation: Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Kyoto, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Murakami
authors.affiliation: Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto, Japan.
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Umeyama
authors.affiliation: Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Kyoto, Japan; Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Imahori
authors.affiliation: Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Kyoto, Japan; Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan. Electronic address: hashidam@pharm.kyoto-u.ac.jp.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Hashida
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D056945:Hep G2 Cells; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods; D051379:Mice; D008969:Molecular Sequence Data; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009369:Neoplasms / Q000473:pathology / Q000628:therapy*; D010455:Peptides / Q000737:chemistry / Q000627:therapeutic use*; D010789:Phototherapy / Q000379:methods
chemical_list: D000970:Antineoplastic Agents; D037742:Nanotubes, Carbon; D010455:Peptides
keywords: Aqueous dispersibility; Near-infrared laser irradiation; Photothermal cancer therapy; Single-walled carbon nanotube; Single-walled carbon nanotube–peptide composite; Tumor ablation