title: Extrachromosomal genetics of Cephalosporium acremonium : II. Development of a mitochondrial DNA hybrid vector replicating in Saccharomyces cerevisiae.
abstract: In order to facilitate strain improvement by concerted breeding in the cephalosporin producing imperfect filamentous fungus Cephalo sporium acremonium, it is attempted to develop a eukaryotic vector for molecular cloning based on mitochondrial (mt) DNA.Fragments of mtDNA from C. acremonium were inserted into a yeast/bacterial hybrid plasmid (pDAM1) lacking a eukaryotic replicon. Six hybrid plasmids were obtained each containing a different mt fragment which together comprise about 60% of the total mtDNA. One of these recombinant plasmid vectors (pCP2) showed a high replication efficiency which is comparable to that of vectors containing yeast 2 μm DNA. This plasmid therefore fulfills the requirements for practical application.
authors.affiliation: Lehrstuhl für Allgemeine Botanik, Ruhr-Universität, Postfach 102148, D-4630, Bochum 1, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Tudzynski
authors.forename: K
authors.initials: K
authors.lastname: Esser