title: An ultra-sensitive colorimetric detection of tetracyclines using the shortest aptamer with highly enhanced affinity.
abstract: A shortened 8-mer ssDNA aptamer was successfully truncated for four different tetracyclines with high affinity. The ultrasensitive colorimetric detection of oxytetracycline using this shortened aptamer was possible, which was about 500-fold enhanced compared to that obtained using the original 76-mer aptamer.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-Gu, Seoul 136-713, Republic of Korea. mbgu@korea.ac.kr.
authors.forename: Young Seop
authors.initials: YS
authors.lastname: Kwon
authors.forename: Nurul Hanun
authors.initials: NH
authors.lastname: Ahmad Raston
authors.forename: Man Bock
authors.initials: MB
authors.lastname: Gu
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry; D004277:DNA, Single-Stranded / Q000235:genetics / Q000378:metabolism; D057230:Limit of Detection; D013752:Tetracycline / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*
chemical_list: D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D013752:Tetracycline