title: PNKP knockdown by RNA interference inhibits herpes simplex virus-1 replication in astrocytes.
abstract: Herpes simplex virus-1 (HSV-1) is a major pathogen that causes various central nervous system (CNS) diseases, including herpes simplex encephalitis and meningitis. According to recent studies, PNKP significantly affects the proliferation of HSV-1 in astrocytes. Here, we used viral proliferation curves to confirm the significant inhibitory effects of PNKP on HSV-1 proliferation. PNKP downregulation was also confirmed by analyzing the transcription of viral genes. We found that PNKP downregulation affects the viral DNA copy number. This study preliminarily confirms that PNKP affects viral proliferation by affecting HSV-1 genome cyclization. These results also suggest that astrocytes play a specific role in preventing HSV-1 infection.
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medicine Science & Peking Union Medical College, Kunming, 650118, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yue
authors.forename: Sujie
authors.initials: S
authors.lastname: Guo
authors.forename: Xia
authors.initials: X
authors.lastname: Cao
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Le
authors.initials: L
authors.lastname: Sun
authors.forename: Longding
authors.initials: L
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Yan
authors.forename: Qihan
authors.initials: Q
authors.lastname: Li
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000821:virology*; D002478:Cells, Cultured; D055785:Gene Knockdown Techniques; D018259:Herpesvirus 1, Human / Q000254:growth & development / Q000502:physiology*; D054884:Host-Pathogen Interactions; D008253:Macaca mulatta; D008297:Male; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D017853:Phosphotransferases (Alcohol Group Acceptor)