title: Occurrence of six virulence-associated genes in Arcobacter species isolated from various sources in Shiraz, Southern Iran.
abstract: In humans, arcobacters are associated with watery diarrhea and septicemia. Although, recently, more cases of diarrhea have been caused by Arcobacter species, very little is known about its pathogenesis. Therefore, the aim of this study was to evaluate the presence of six putative Arcobacter virulence genes (cadF, ciaB, cj1349, mviN, pldA, and tlyA), in a set of 113 Arcobacter butzleri, 40 Arcobacter cryaerophilus, and 15 Arcobacter skirrowii isolates that were recovered from various origins. The isolates were confirmed on the basis of polymerase chain reaction (PCR) amplification of genus and species specific PCR for determining three species. For confirmed isolates, PCR was carried out for the presence of virulence genes using specific primers. All A. butzleri isolates carried all six genes. For A. cryaerophilus and A. skirrowii, the cadF gene was detected just in 55 and 53.3%, ciaB in 97.5 and 86.6%, cj1349 in 45 and 60%, mviN in 90 and 80%, pldA in 32.5 and 13.3%, and tlyA in 37.5 and 40%, respectively. For A. cryaerophilus and A. skirrowii, the genes ciaB and mviN were significantly more prevalent than other virulence markers (P ≤ 0.05). The findings revealed that many of the important Arcobacter strains (86%) have these putative virulence genes which can be potential pathogenic properties for humans.
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, Shiraz University, 71345-1731 Shiraz, Iran. Electronic address: Mtabatabaei2003@yahoo.co.uk.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Tabatabaei
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, Shiraz University, 71345-1731 Shiraz, Iran. Electronic address: Shirzad_hessam@yahoo.com.
authors.forename: Hesamaddin
authors.initials: H
authors.lastname: Shirzad Aski
authors.affiliation: School of Veterinary Medicine, Shiraz University, Shiraz, Iran. Electronic address: h.shayegh92@yahoo.com.
authors.forename: Hossein
authors.initials: H
authors.lastname: Shayegh
authors.affiliation: Department of Food Hygiene, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran. Electronic address: Khoshbakht.r@gmail.com.
authors.forename: Rahem
authors.initials: R
authors.lastname: Khoshbakht
mesh_terms: D000003:Abattoirs; D000818:Animals; D020640:Arcobacter / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D005798:Genes, Bacterial; D007492:Iran; D058751:Livestock; D008460:Meat / Q000382:microbiology; D016133:Polymerase Chain Reaction; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D037521:Virulence Factors
keywords: Arcobacter species; Virulence genes