title: Hepatocyte isolation from unused/rejected livers for transplantation: initial step toward hepatocyte transplantation, the first experience from iran.
abstract: BACKGROUND
  Hepatocyte transplantation is being used in patients with liver-based metabolic disorders and acute liver failure. Hepatocytes can be isolated from unused/rejected livers under sterile conditions.
  
  
  OBJECTIVE
  The quality of the hepatocytes is very important and the main and initial step in hepatocyte transplantation is hepatocyte isolation. In this study we tried to set up the methods of hepatocyte isolation in order to use the high quality cells in acute liver failure or congenital metabolic disorders.
  
  
  METHODS
  In this study, during a year, hepatocytes were isolated from 7 unused/rejected livers among more than 300 harvested livers in Shiraz University of Medical Sciences. The two step collagenase perfusion method was used under GMP (Good manufacturing practice) for hepatocyte isolation.
  
  
  RESULTS
  Highly quality hepatocytes with high viability and low contamination were isolated. The mean viability was 71.8% ± 21.7. In the first 4 cases microbial contamination by Staphylococci, Diphtheroid and Klebsiella was detected, however the last 3 cases were free of any micro organisms. After 5 weeks of cryopreservation in -140°C, the cell viability was still acceptable.
  
  
  CONCLUSIONS
  Hepatocyte isolation can be performed as the main and initial step for cell transplantation from unused/rejected liver. It is the first experience in Iran.
authors.affiliation: Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran.
authors.forename: Mahdokht
authors.initials: M
authors.lastname: Hossein Aghdaie
authors.forename: Bita
authors.initials: B
authors.lastname: Geramizadeh
authors.forename: Negar
authors.initials: N
authors.lastname: Azarpira
authors.forename: Elahe
authors.initials: E
authors.lastname: Esfandiari
authors.forename: Masoume
authors.initials: M
authors.lastname: Darai
authors.forename: Marjan
authors.initials: M
authors.lastname: Rahsaz
authors.forename: Saman
authors.initials: S
authors.lastname: Nikeghbalian
authors.forename: Seyed Ali
authors.initials: SA
authors.lastname: Malekhosseini
keywords: Hepatocyte; Liver; Transplantation