title: Two appendages homologous between basal bodies and centrioles are formed using distinct Odf2 domains.
abstract: Ciliogenesis is regulated by context-dependent cellular cues, including some transduced through appendage-like structures on ciliary basal bodies called transition fibers and basal feet. However, the molecular basis for this regulation is not fully understood. The Odf2 gene product, ODF2/cenexin, is essential for both ciliogenesis and the formation of the distal and subdistal appendages on centrioles, which become basal bodies. We examined the effects of Odf2 deletion constructs on ciliogenesis in Odf2-knockout F9 cells. Electron microscopy revealed that ciliogenesis and transition fiber formation required the ODF2/cenexin fragment containing amino acids (aa) 188-806, whereas basal foot formation required aa 1-59 and 188-806. These sequences also formed distal and subdistal appendages, respectively, indicating that the centriole appendages are molecularly analogous to those on basal bodies. We used the differential formation of appendages by Odf2 deletion constructs to study the incorporation and function of molecules associated with each appendage type. We found that transition fibers and distal appendages were required for ciliogenesis and subdistal appendages stabilized the centrosomal microtubules.
authors.affiliation: Laboratory of Biological Science, Graduate School of Frontier Biosciences and Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Tateishi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yamazaki
authors.forename: Tomoki
authors.initials: T
authors.lastname: Nishida
authors.forename: Shin
authors.initials: S
authors.lastname: Watanabe
authors.forename: Koshi
authors.initials: K
authors.lastname: Kunimoto
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ishikawa
authors.forename: Sachiko
authors.initials: S
authors.lastname: Tsukita
mesh_terms: D000818:Animals; D064652:Basal Bodies / Q000378:metabolism*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002502:Centrioles / Q000235:genetics / Q000378:metabolism*; D002923:Cilia / Q000378:metabolism*; D054278:Embryonal Carcinoma Stem Cells; D055786:Gene Knockout Techniques; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008870:Microtubules / Q000378:metabolism; D017434:Protein Structure, Tertiary
chemical_list: D006360:Heat-Shock Proteins; C490106:Odf2 protein, mouse