title: [Cytochrome oxidase I gene sequences that cause hypodermosis cattle species by PCR-RFLP technique investigation].
abstract: OBJECTIVE
  The importance for the health of cattle Hypodermosis (Nokra) species that cause disease by Polymerase Chain Reaction- Restriction Fragment Length Polymorphism (PCR-RFLP) method was investigated.
  
  
  METHODS
  The molecular analysis of Hypoderma species specific sequences located on the Cytochrome C Oxidase I (COI) (mtDNA) gene by PCR-RFLP method was successfully carried out.
  
  
  RESULTS
  DNAs were obtained using the extraction kit and Polymerase chain reaction (PCR) foe amplification of the mitochondrial DNA gene yielded for 688bp COI gene on agarose gel. Using the PCR amplification and HinfI, RsaI and TaqI restriction enzymes, mitochondrial DNA gene regions were amplified with the RFLP technique.
  
  
  CONCLUSIONS
  The mitochondrial COI gene region of the genus Hypoderma for the molecular identification of potential answers questions, such as the level, which indicates that it may be very useful. In this study, in the city of Van, it is also important that the Hypoderma species be defined in terms of the first molecular study.
authors.affiliation: Yüzüncü Yıl Üniversitesi Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Van, Türkiye.
authors.forename: Bekir
authors.initials: B
authors.lastname: Oğuz
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D004272:DNA, Mitochondrial / Q000235:genetics; D004175:Diptera / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D017344:Genes, Insect; D007000:Hypodermyiasis / Q000469:parasitology / Q000662:veterinary; D008928:Mitochondria / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV