title: Cellular fractionation--yeast cells.
abstract: In proteomics research, one essential step among enrichment techniques is subcellular fractionation. This is of special importance for analyzing intracellular organelles and multiprotein complexes. Subcellular fractionation is a flexible and adjustable approach to reducing sample complexity and is most efficiently combined with high-resolution 2-D gel/mass spectrometry analysis as well as with gel-independent techniques.
authors.affiliation: Merck KGaA, Darmstadt, Germany. Electronic address: Joerg.von.Hagen@merck.de.
authors.forename: Joerg
authors.initials: J
authors.lastname: von Hagen
authors.forename: Uwe
authors.initials: U
authors.lastname: Michelsen
mesh_terms: D002021:Buffers; D002458:Cell Fractionation / Q000295:instrumentation / Q000379:methods*; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D011523:Protoplasts / Q000166:cytology; D013347:Subcellular Fractions; D015003:Yeasts / Q000166:cytology*
chemical_list: D002021:Buffers
keywords: Cytoskeleton extraction buffer; Membrane protein extraction buffer; Nuclear protein extraction buffer; Protoplast buffer and cytoplasmic protein extraction buffer; Subcellular fractionation of yeast protoplasts; Yeast cells; Yeast protoplast formation