title: Antineoplastic activity of strawberry (Fragaria×ananassa Duch.) crude extracts on B16-F10 melanoma cells.
abstract: The antiproliferative and differentiation potential of anthocyanin-rich strawberry fruit crude extracts (SE) were investigated on B16-F10 murine melanoma cells. Treatment of melanoma cells with SE produced a remarkable reduction of cell proliferation, paralleled with both the lowering of the intracellular levels of polyamine, and the enhancement of tissue transglutaminase (TG2, EC 2.3.2.13) activity (used as a differentiation marker). To gain further insight into profiling altered protein expression as a potential biomarker of the SE action on melanoma cells, analysis of the proteomic profile was performed on the treated B16-F10 cells, compared to the control. Following SE treatment, 30 proteins resulted up-regulated, and 87 proteins were down-regulated. In particular proteins overexpressed in cancer cells, involved in tumor progression and metabolism, were down-regulated. The possibility that SE may affect the Warburg effect in B16-F10 melanoma cells is discussed.
authors.affiliation: Department of Biology, University of Rome "Tor Vergata", Via della Ricerca Scientifica, 00133 Rome, Italy. forni@uniroma2.it.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Forni
authors.forename: Roberto
authors.initials: R
authors.lastname: Braglia
authors.forename: Nadia
authors.initials: N
authors.lastname: Mulinacci
authors.forename: Andrea
authors.initials: A
authors.lastname: Urbani
authors.forename: Maurizio
authors.initials: M
authors.lastname: Ronci
authors.forename: Angelo
authors.initials: A
authors.lastname: Gismondi
authors.forename: Claudio
authors.initials: C
authors.lastname: Tabolacci
authors.forename: Bruno
authors.initials: B
authors.lastname: Provenzano
authors.forename: Alessandro
authors.initials: A
authors.lastname: Lentini
authors.forename: Simone
authors.initials: S
authors.lastname: Beninati
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000494:pharmacology* / Q000627:therapeutic use; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D031985:Fragaria / Q000737:chemistry*; D019204:GTP-Binding Proteins / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D008545:Melanoma / Q000188:drug therapy / Q000473:pathology*; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D011073:Polyamines / Q000378:metabolism; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D040901:Proteomics; D011503:Transglutaminases / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D000970:Antineoplastic Agents; D010936:Plant Extracts; D011073:Polyamines; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins