title: Cloning, characterization, hypoxia and heat shock response of hypoxia inducible factor-1 (HIF-1) from the small abalone Haliotis diversicolor.
abstract: In this study, hypoxia inducible factor-1α (HIF-1α) and hypoxia inducible factor-1β (HIF-1β) from small abalone Haliotis diversicolor were cloned. The cDNA of H. diversicolor HIF-1α (HdHIF-1α) is 2,833 bp encoding a protein of 711aa and H. diversicolor HIF-1β (HdHIF-1β) is 1919 bp encoding a protein of 590aa. Similar to other species' HIF-1, HdHIF-1 has one basic helix-loop-helix (bHLH) domain and two Per-Arnt-Sim (PAS) domains, and HdHIF-1α has a oxygen-dependent degradation domain (ODDD) with two proline hydroxylation motifs and a C-terminal transactivation domain (C-TAD) with an asparagine hydroxylation motif. Under normoxic conditions, HdHIF-1α and HdHIF-1β mRNAs were constitutively present in all examined tissues. Under hypoxia (2.0mg/L DO at 25°C) stress, HdHIF-1α expression was up-regulated in gills at 4h, 24h and 96 h, and in hemocytes at 24h and 96 h, while HdHIF-1β remained relatively constant. Under thermal stress (31°C), HdHIF-1α expression was significantly increased in gills at 4h, and hemocytes at 0 h and 4 h, while HdHIF-1β expression still remained relatively constant. These results suggested that HIF-1α may play an important role in adaption to poor environment in H. diversicolor.
authors.affiliation: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen 361021, China.
authors.forename: Xiuhong
authors.initials: X
authors.lastname: Cai
authors.affiliation: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen 361021, China.
authors.forename: Yitao
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen 361021, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen 361021, China.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen 361021, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Zou
authors.affiliation: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen 361021, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Wang
authors.affiliation: Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen 361021, China. Electronic address: ylwang@jmu.edu.cn.
authors.forename: Yilei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Natural Sciences and Mathematics, State University of New York at Cobleskill, NY 12043, USA. Electronic address: zhangziping@hotmail.com.
authors.forename: Ziping
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D049851:Gastropoda / Q000235:genetics* / Q000378:metabolism; D005880:Gills / Q000378:metabolism; D018869:Heat-Shock Response / Q000235:genetics*; D006434:Hemocytes / Q000378:metabolism; D000860:Hypoxia / Q000235:genetics* / Q000378:metabolism; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D015854:Up-Regulation
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator
keywords: ARNT; C-TAD; C-terminal transactivation domain; CBP; CREB1-binding protein; DO; Gene expression; HIF-1α; HIF-1β; HRE; Haliotis diversicolor; Hypoxia; N-TAD; N-terminal transactivation domain; NRR; NRU; ODDD; ORF; PHDs; Pro; RACE; THC; Thermal stress; UTR; aryl hydrocarbon receptor nuclear translocator; bHLH/PAS; basic helix–loop–helix/Per-Arnt-Sim; dissolved oxygen; hemocyte count; hypoxia inducible factor-1α; hypoxia inducible factor-1β; hypoxia response element; neutral red retention; neutral red uptake; open reading frame; oxygen dependent degradation domain; pVHL; proline; proline hydroxylases; qRT-PCR; quantitative real-time PCR; rapid amplification of cDNA ends; untranslated region; von Hippel–Lindau protein