title: Significance of the zygotic seed coat on quiescence and desiccation tolerance of Medicago sativa L. somatic embryos.
abstract: The influence of the zygotic seed coat on precocious germination and desiccation tolerance of somatic embryos has been studied using alfalfa (Medicago sativa L.). When cultured in contact with somatic embryos, seed coats at certain developmental stages inhibited precocious germination and induced desiccation tolerance in the somatic embryos. Germination of somatic embryos was inhibited by seed coats at the age of 16-26 days after pollination (DAP) and desiccation tolerance was induced after 20-26 DAP. Both phenomena were related to the synthesis of abscisic acid in the seed coat. The absence of a quiescent phase and desiccation tolerance in alfalfa somatic embryos may be related to the lack of developmental control by the seed coat.
authors.affiliation: Department of Horticultural Science, University of Guelph, N1G 2W1, Guelph, Ontario, Canada.
authors.forename: T
authors.initials: T
authors.lastname: Senaratna
authors.forename: P K
authors.initials: PK
authors.lastname: Saxena
authors.forename: M V
authors.initials: MV
authors.lastname: Rao
authors.forename: J
authors.initials: J
authors.lastname: Afele