title: Plant regeneration from rice (Oryza sativa L.) embryogenic suspension cells cryopreserved by vitrification.
abstract: Rice cells were precultured for 10 d in medium containing 60 g/L sucrose and subsequently for 1 d in medium supplemented with 0. 4 M sorbitol. After loading with 25%PVS2 at 22°C for 10 min and dehydration in 100%PVS2 at 0°C for 7. 5 min,they were plunged into liquid nitrogen directly. Survival was 45. 0 ±5.1% (based on the reduction of triphenyl tetrazolium chloride)following warming and unloading. For regrowth, cells were plated on semi-solid medium replenished with 40%(w/v) starch for 2d prior to reculture. Cell suspensions were reestablished and plants were regenerated from recovered cells. Twenty eight plants set seeds in the greenhouse.
authors.affiliation: Department of Biological Science and Biotechnology, Hangzhou University, 310028, Hangzhou, P.R.China.
authors.forename: C N
authors.initials: CN
authors.lastname: Huang
authors.forename: J H
authors.initials: JH
authors.lastname: Wang
authors.forename: Q S
authors.initials: QS
authors.lastname: Yan
authors.forename: X Q
authors.initials: XQ
authors.lastname: Zhang
authors.forename: Q F
authors.initials: QF
authors.lastname: Yan