title: Improved method for the transformation of Arabidopsis thaliana with chimeric dihydrofolate reductase constructs which confer methotrexate resistance.
abstract: A modified root explant transformation method has been developed that is effective in producing transgenic Arabidopsis thaliana plants which are methotrexate resistant due to the integration of T-DNA vectors containing a chimeric dihydrofolate reductase gene. Molecular analysis shows that transformed methotrexate resistant plants contain the expected T-DNA construct with the chimeric gene. This transformation method also works well with other plant selectable markers, including hygromycin phosphotransferase and neomycin phosphotransferase II.
authors.affiliation: Max-Planck-Institut für Züchtungsforschung, Carl-von-Linne Weg 10, W-5000, Köln 30, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Kemper
authors.forename: C
authors.initials: C
authors.lastname: Grevelding
authors.forename: J
authors.initials: J
authors.lastname: Schell
authors.forename: R
authors.initials: R
authors.lastname: Masterson