title: Linkage analysis of er-1, a recessive Pisum sativum gene for resistance to powdery mildew fungus (Erysiphe pisi D.C.).
abstract: Linkage analysis was used to determine the genetic map location of er-1, a recessive gene conditioning resistance to powdery mildew, on the Pisum sativum genome. Genetic linkage was demonstrated between er-1 and linkage group 6 markers after analyzing the progeny of two crosses, an F2 population and a set of recombinant inbred lines. The classes of genetic markers surrounding er-1 include RFLP, RAPD and allozyme markers as well as the morphological marker Gty. A RAPD marker tightly linked to er-1 was identified by bulked segregant analysis. After DNA sequence characterization, specific PCR primers were designed to convert this RAPD marker into a sequence characterized amplified region (SCAR).
authors.affiliation: New Zealand Institute for Crop & Food Research Ltd, Private Bag 4704, Christchurch, New Zealand.
authors.forename: G M
authors.initials: GM
authors.lastname: Timmerman
authors.forename: T J
authors.initials: TJ
authors.lastname: Frew
authors.forename: N F
authors.initials: NF
authors.lastname: Weeden
authors.forename: A L
authors.initials: AL
authors.lastname: Miller
authors.forename: D S
authors.initials: DS
authors.lastname: Goulden