title: [Effect of calcium on medium alkalinization induced by salicylic acid in Salvia miltiorrhiza suspension cultures].
abstract: We studied medium alkalinization in Salvia miltiorrhiza suspension cultures treated with salicylic acid and the effect of Ca2+ in this process through application of calcium channel antagonists (Verapamil, LaCl3, LiCl, 2-APB) and ionophore A23187. The results show that salicylic acid could induce significant medium alkalinization in S. miltiorrhiza culture. Verapamil and LaCl3 or LiCl and 2-APB, two different groups of calcium channel antagonist, significantly inhibited the medium alkalinization induced by salicylic acid. However, the suppression effect of verapamil or LaCl3 on medium alkalinization induced by salicylic acid was higher than that of LiCl or 2-APB. When two types of calcium channel inhibitor (LaCl3 and 2-APB) were used together, the medium alkalinization induced by salicylic acid was completely suppressed and even reduced the pH in medium. On the other hand, A23187 could promote the medium alkalinization. Based on the results above, we speculated that salicylic acid could induce significant medium alkalinization in S. miltiorrhiza culture, depending on the calcium from both extracell and intracell. Moreover, calcium from extracell plays a more dominant role in this process. Reveal of relationship in this research between Ca2+ and medium alkalinization can provide theory evidence for mechanism of the plant secondary metabolism.
authors.affiliation: College of Life Sciences, Northwest Agriculture and Forestry University, Yangling 712100, Shaanxi, China.
authors.forename: Liancheng
authors.initials: L
authors.lastname: Liu
authors.forename: Cong
authors.initials: C
authors.lastname: Wang
authors.forename: Juan'e
authors.initials: J
authors.lastname: Dong
authors.forename: Hui
authors.initials: H
authors.lastname: Su
authors.forename: Zequn
authors.initials: Z
authors.lastname: Zhuo
authors.forename: Yaxin
authors.initials: Y
authors.lastname: Xue
mesh_terms: D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000737:chemistry*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D061207:Calcium Ionophores / Q000494:pharmacology; D018929:Cell Culture Techniques; D003470:Culture Media / Q000737:chemistry*; D020156:Salicylic Acid / Q000494:pharmacology*; D027561:Salvia miltiorrhiza / Q000378:metabolism*; D014700:Verapamil / Q000494:pharmacology
chemical_list: D002121:Calcium Channel Blockers; D061207:Calcium Ionophores; D003470:Culture Media; D000001:Calcimycin; D014700:Verapamil; D020156:Salicylic Acid; D002118:Calcium