title: Ploidy-dependent genomic stability in the tissue cultures of ornamental Phlox drummondii Hook.
abstract: Comparisons of the chromosome numbers, 2C nuclear DNA amounts and karyomorphology were made in explant cultures of diploid (2n = 2x = 14) and autotetraploid (2n = 4x = 28) Phlox drummondii. In 6-36 week old calli derived from diploid internodal segment explants, and in cells of root tips regenerated from such callus, marked differences were observed in chromosome number. The chromosome numbers ranged from 2n = 14 to 2n = 100 and DNA amounts from 8.20 to 63.20 pg in the diploid derived callus, while the extent of variation was much reduced in the regenerated roots. In contrast, the autotetraploid cultures were characterised by the maintenance of the same chromosome number and DNA amounts as the mother plant. Modified chromosome structures were not apparent in any of the cultures. The possible reasons for the chromosomal instability at the diploid level and stability at the tetraploid level are discussed.
authors.affiliation: Laboratory of Cellular and Molecular Cytogenetics, Department of Botany, University of Delhi, 110007, Delhi, India.
authors.forename: V G
authors.initials: VG
authors.lastname: Raja
authors.forename: K K
authors.initials: KK
authors.lastname: Koul
authors.forename: S N
authors.initials: SN
authors.lastname: Raina
authors.forename: A
authors.initials: A
authors.lastname: Parida