title: Interleukin-1β enhances neuronal vulnerability to proNGF-mediated apoptosis by increasing surface expression of p75(NTR) and sortillin.
abstract: Many types of injury such as seizure, ischemia, and oxidative stress cause upregulation of the p75 neurotrophin receptor (p75(NTR)) in brain neurons, where it promotes apoptosis, however the mechanism by which p75(NTR) is regulated under these conditions is not well understood. Proinflammatory cytokines such as interleukin-1β (IL-1β) are highly produced under these injury conditions and, in particular, are expressed rapidly in the rat hippocampus after seizure. IL-1β is known to increase neuronal vulnerability under many conditions, although it does not directly induce neuronal death. Recently, we have shown that these cytokines regulate p75(NTR) induction both in neurons and astrocytes in vitro. Here, we show that IL-1β infusion into the brain induces p75(NTR) in neurons of the CA1 area of the hippocampus. While IL-1β induction of p75(NTR) is not sufficient to induce cell death, we demonstrate that IL-1β primes the neurons by recruiting p75(NTR) and its coreceptor sortilin to the cell surface, making the neurons more vulnerable to subsequent challenge by proNGF. These results suggest a mechanism by which IL-1β exacerbates neuronal death following injury.
authors.affiliation: Department of Biological Sciences, Rutgers University, Newark, NJ 07102, United States.
authors.forename: S
authors.initials: S
authors.lastname: Choi
authors.affiliation: Department of Biological Sciences, Rutgers University, Newark, NJ 07102, United States. Electronic address: wilmaf@andromeda.rutgers.edu.
authors.forename: W J
authors.initials: WJ
authors.lastname: Friedman
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D019921:Biotinylation; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D006624:Hippocampus / Q000166:cytology; D006801:Humans; D020287:In Situ Nick-End Labeling; D053583:Interleukin-1beta / Q000494:pharmacology*; D008297:Male; D009414:Nerve Growth Factors / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects*; D011498:Protein Precursors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020800:Receptor, Nerve Growth Factor / Q000235:genetics / Q000378:metabolism*; D000099183:Sortilin
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D053583:Interleukin-1beta; D009414:Nerve Growth Factors; D011498:Protein Precursors; D012333:RNA, Messenger; D020800:Receptor, Nerve Growth Factor; D000099183:Sortilin; C514218:pro-nerve growth factor, rat
keywords: CSF; EDTA; IL-1β; NGF; TdT; apoptosis; brain injury; cerebrospinal fluid; ethylenediaminetetraacetic acid; interleukin-1; interleukin-1β; neurotrophin; p75; p75 neurotrophin receptor; p75(NTR); terminal deoxynucleotidyl transferase