title: RFLP analysis of asymmetric somatic hybrids between Solanum tuberosum and irradiated S. brevidens.
abstract: The nuclear genome composition of five asymmetric somatic hybrids, obtained by fusion of leaf protoplasts from Solanum tuberosum and gamma-irradiated leaf protoplasts from S. brevidens, have been analyzed at the molecular level. An analysis of 21 loci using linkage group-specific restriction fragment length polymorphism (RFLP) was included in the study. All five hybrids contained a complete set of the loci studied from S. tuberosum. The degree of elimination of alleles from the irradiated S. brevidens donor genome ranged from 10-65% in the five asymmetric hybrids analyzed. The detection of incomplete chromosomes, as well as non-parental bands in Southern hybridizations with RFLP markers, revealed extensive chromosome rearrangements in the asymmetric hybrids.
authors.affiliation: Department of Plant Production, University of Helsinki, Box 27, SF-00014, Finland.
authors.forename: Y S
authors.initials: YS
authors.lastname: Xu
authors.forename: E
authors.initials: E
authors.lastname: Pehu