title: A highly sensitive LED-induced chemiluminescence platform for aptasensing of platelet-derived growth factor.
abstract: In this work, we demonstrated a simple and highly sensitive LED-induced chemiluminescence (LED-CL) platform for aptasensing of proteins. It was based on the fact that fluorescein isothiocyanate (FITC) strongly catalyzed the reaction between luminol and dissolved oxygen under LED irradiation. Using FITC as a new catalytic tag, a highly sensitive sandwich type LED-CL aptasensor was fabricated for quantification of the model protein (platelet-derived growth factor-BB, PDGF-BB). Under optimized conditions, the assay for PDGF-BB was linear from 0.1 nM to 100 nM, and the detection limit was as low as 50 pM, which is better than most of other aptamer-based assays without an amplification procedure. Thrombin, bovine serum albumin, hemoglobin, cytochrome C and adenosine triphosphate showed no obvious interferences for the determination of PDGF-BB. The advantages of high sensitivity and selectivity provided by this LED-CL aptasensor will facilitate its wide application in protein assays.
authors.affiliation: College of Material and Chemistry & Chemical Engineering, Chengdu University of Technology, Chengdu 610059, China. zhangxinfeng09@cdut.cn.
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Shuxia
authors.initials: S
authors.lastname: Xu
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D010982:Platelet-Derived Growth Factor / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D049408:Luminescent Agents; D010982:Platelet-Derived Growth Factor