title: Qualitative and quantitative analysis of seven oligosaccharides in Morinda officinalis using double-development HPTLC and scanning densitometry.
abstract: The quality of Morindaofficinalis, which has been used as a Yang-tonic agent for a long time in China, can be evaluated. A double-development high performance thin layer chromatography (HPTLC) method has been established to simultaneously analyze quality and quantity of seven inulin-type oligosaccharides (DP=3-9) in Morindaofficinalis. The chromatography was performed on a silica gel 60 plate with the 7:5:2:1 proportion (v/v) of n-butanol-isopropanol-water-acetic acid for the first and second developments, respectively. The bands were visualized by the reaction with aniline-diphenylamine-phosphoric acid solution and analyzed by densitometric TLC at 540 nm. Quantification of seven oligosaccharides was achieved by densitometry at 540 nm. The investigated standard sugar had good linearity (R2>0.99) within test ranges. The amounts of seven oligosaccharides were calculated by the relative correction factor (RCF). Therefore, the developed TLC method could be used for quality control of Morindaofficinalis.
authors.affiliation: School of Pharmacy, Jiangxi Science and Technology Normal University, Nanchang, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Jun
authors.initials: J
authors.lastname: Chang
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Dan
authors.initials: D
authors.lastname: Cheng
authors.forename: Peng-Wu
authors.initials: PW
authors.lastname: Zheng
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D019840:2-Propanol / Q000737:chemistry; D019342:Acetic Acid / Q000737:chemistry; D002138:Calibration; D002681:China; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003720:Densitometry; D032066:Morinda / Q000737:chemistry*; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis; D011786:Quality Control; D012044:Regression Analysis; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D009844:Oligosaccharides; D010936:Plant Extracts; D014867:Water; D012822:Silicon Dioxide; D020001:1-Butanol; D019840:2-Propanol; D019342:Acetic Acid
keywords: Morindaofficinalis; Quantitative determination; TLC; oligosaccharide