title: The white-rot fungus pleurotus ostreatus transformant overproduced intracellular cAMP and laccase.
abstract: Transformation of Pleurotus ostreatus PC9 with the mutated heterotrimeric G protein alpha subunit (Gα) gene resulted in higher laccase (Lac) activity and intracellular cyclic adenosine monophosphate (cAMP) concentrations as compared to those in wild-type PC9. The transformant also exhibited higher Lac activity than the wild type when cultured in a medium containing known Lac inducers CuSO4 and ferulic acid.
authors.affiliation: Environmental Science Graduate School, The University of Shiga Prefecture.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yao
authors.forename: Takaiku
authors.initials: T
authors.lastname: Sakamoto
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Honda
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Kagotani
authors.forename: Kousuke
authors.initials: K
authors.lastname: Izumitsu
authors.forename: Kazumi
authors.initials: K
authors.lastname: Suzuki
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Irie
mesh_terms: D019327:Copper Sulfate / Q000494:pharmacology; D003373:Coumaric Acids / Q000494:pharmacology; D003470:Culture Media; D000242:Cyclic AMP / Q000096:biosynthesis*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D044385:GTP-Binding Protein alpha Subunits / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D042845:Laccase / Q000096:biosynthesis / Q000235:genetics*; D009154:Mutation; D020076:Pleurotus / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D014170:Transformation, Genetic; D019076:Transgenes
chemical_list: D003373:Coumaric Acids; D003470:Culture Media; D005656:Fungal Proteins; D044385:GTP-Binding Protein alpha Subunits; C004999:ferulic acid; D000242:Cyclic AMP; D042845:Laccase; D019327:Copper Sulfate