title: Permeability characteristics of cell-membrane pores induced by ostreolysin A/pleurotolysin B, binary pore-forming proteins from the oyster mushroom.
abstract: Proteins from the oyster mushroom, 15 kDa ostreolysin A (OlyA), and 59 kDa pleurotolysin B (PlyB) with a membrane attack complex/perforin (MACPF) domain, damage cell membranes as a binary cytolytic pore-forming complex. Measurements of single-channel conductance and transmembrane macroscopic current reveal that OlyA/PlyB form non-selective ion-conducting pores with broad, skewed conductance distributions in N18 neuroblastoma and CHO-K1 cell membranes. Polyethylene-glycol 8000 (hydrodynamic radius of 3.78 nm) provides almost complete osmotic protection against haemolysis, which strongly suggests a colloid-osmotic type of erythrocyte lysis. Our data indicate that OlyA/PlyB form transmembrane pores of varied sizes, as other pore-forming proteins with a MACPF domain.
authors.affiliation: Laboratoire de Neurobiologie et Développement - UPR3294, Institut de Neurobiologie Alfred Fessard (INAF) - FRC2118, Centre National de la Recherche Scientifique (CNRS), 91198 Gif sur Yvette, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Schlumberger
authors.affiliation: Institute of Physiology, Medical Faculty, University of Maribor, 2000 Maribor, Slovenia.
authors.forename: Katarina Črnigoj
authors.initials: KČ
authors.lastname: Kristan
authors.affiliation: Department of Biology, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia.
authors.forename: Katja
authors.initials: K
authors.lastname: Ota
authors.affiliation: Institute of Physiology, Pharmacology and Toxicology, Veterinary Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia.
authors.forename: Robert
authors.initials: R
authors.lastname: Frangež
authors.affiliation: Laboratoire de Neurobiologie et Développement - UPR3294, Institut de Neurobiologie Alfred Fessard (INAF) - FRC2118, Centre National de la Recherche Scientifique (CNRS), 91198 Gif sur Yvette, France.
authors.forename: Jordi
authors.initials: J
authors.lastname: Molgό
authors.affiliation: Department of Biology, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia.
authors.forename: Kristina
authors.initials: K
authors.lastname: Sepčić
authors.affiliation: Laboratoire de Neurobiologie et Développement - UPR3294, Institut de Neurobiologie Alfred Fessard (INAF) - FRC2118, Centre National de la Recherche Scientifique (CNRS), 91198 Gif sur Yvette, France.
authors.forename: Evelyne
authors.initials: E
authors.lastname: Benoit
authors.affiliation: Department of Biology, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia. Electronic address: peter.macek@bf.uni-lj.si.
authors.forename: Peter
authors.initials: P
authors.lastname: Maček
mesh_terms: D000818:Animals; D016466:CHO Cells; D002417:Cattle; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects* / Q000502:physiology; D002463:Cell Membrane Permeability / Q000187:drug effects* / Q000502:physiology; D006224:Cricetinae; D003412:Cricetulus; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D005656:Fungal Proteins / Q000378:metabolism / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000378:metabolism / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008854:Microscopy, Electron; D018408:Patch-Clamp Techniques; D020076:Pleurotus / Q000378:metabolism; D018272:Porins / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D005656:Fungal Proteins; D006460:Hemolysin Proteins; D018272:Porins; C461280:ostreolysin; C586632:pleurotolysin B, oyster mushroom
keywords: Electrophysiology; Membrane attack complex/perforin (MACPF) domain; Pleurotus ostreatus; Pore-forming protein