title: Alpha-internexin and altered CIC expression as a supportive diagnostic marker for oligodendroglial tumors with the 1p/19q co-deletion.
abstract: α-Internexin (INA) has been proposed as a biomarker of oligodendroglial tumors with the 1p/19q co-deletion. On the other hand, sequence studies have recently linked the CIC mutation and subsequent altered CIC expression to the 1p/19q co-deletion in oligodendroglial tumors. We assessed the usability of combination immunohistochemical analysis using CIC and INA as a surrogate tool for the 1p/19q status in 39 cases of oligodendroglial tumors. The positive expression of INA was observed in 10 cases (52 %) of oligodendroglial tumors with the 1p/19q co-deletion, and in only 3 cases of oligodendroglial tumors without the 1p/19q co-deletion (15 %, P = 0.012). The lack of CIC expression was detected in 13 cases (68 %) of oligodendroglial tumors with the 1p/19q co-deletion, and in only 1 case of oligodendroglial tumors without the 1p/19q co-deletion (5 %, P < 0.0001). Combined immunohistochemical analysis assessed by INA expression and/or the lack of CIC expression was strongly associated with the 1p/19q co-deletion in oligodendroglial tumors, indicating a potential surrogate marker of the 1p/19q state. Although combined immunohistochemical analysis cannot be totally replaced by molecular genetic analysis as a definitive diagnostic technique, it may contribute to a steady morphological diagnosis by predicting the 1p/19q state in oligodendroglial tumors.
authors.affiliation: Department of Human Pathology, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, 371-8511, Japan, masaya.nagaishi@gmail.com.
authors.forename: Masaya
authors.initials: M
authors.lastname: Nagaishi
authors.forename: Aya
authors.initials: A
authors.lastname: Suzuki
authors.forename: Sumihito
authors.initials: S
authors.lastname: Nobusawa
authors.forename: Hideaki
authors.initials: H
authors.lastname: Yokoo
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Nakazato
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics*; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D002872:Chromosome Deletion; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D005260:Female; D015870:Gene Expression / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D007381:Intermediate Filament Proteins / Q000032:analysis* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009837:Oligodendroglioma / Q000175:diagnosis* / Q000235:genetics*; D012097:Repressor Proteins / Q000032:analysis* / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; C498610:CIC protein, human; D007381:Intermediate Filament Proteins; D012097:Repressor Proteins; C046643:alpha-internexin