title: miR-184 functions as an oncogenic regulator in hepatocellular carcinoma (HCC).
abstract: Dysregulation of miRNAs has been proved to play a key role in carcinogenesis or tumor progression. In hepatocellular carcinoma (HCC), a number of miRNAs was reported to be related to the occurrence and development of HCC. Especially, miRNA-122, a liver-specific miRNA, has been elaborated its role in HCC. However, these studies was not involved in the effect of miRNA-184 on HCC. In the present study, we aimed to detect the miRNA-184 expression in HCC tissues and further evaluate the in vitro effect of miR-184 inhibition in HCC cells HepG2. We found that miR-184 expression was significantly high in HCC tissues, but INPPL1 expression was obviously low. Subsequently, INPPL1 was identified as a target of miRNA-184 by bioinformatics and dual luciferase assay. Moreover, after transfected with anti-miR-184 in HepG2 cells, INPPL1 expression was significantly decreased both at mRNA and protein levels. Additionally, we also proved that miR-184 silencing inhibited cellular proliferation by over expressing INPPL1 and induced HepG2 apoptosis by caspase 3/7. Together, our result was shown that miR-184 might play a part in proliferation of HCC cells by INPPL1 loss and act as antiapoptotic factor in the development of HCC by inhibiting the activities of caspases 3/7. Therefore, further elucidation of miRNA-184 silencing is helpful for understanding the pathogenesis of HCC and devising new strategies for its prevention and therapy.
authors.affiliation: Jining Medical University, Jining 272067, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Gao
authors.affiliation: Linyi People's Hospital, Linyi 276003, PR China.
authors.forename: Kuanjiang
authors.initials: K
authors.lastname: Gao
authors.affiliation: Jining Medical University, Jining 272067, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Jining Medical University, Jining 272067, PR China.
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Jining Medical University, Jining 272067, PR China. Electronic address: linli6711@sina.com.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D049109:Cell Proliferation; D015536:Down-Regulation; D017930:Genes, Reporter; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D049409:Luciferases, Firefly / Q000235:genetics; D049410:Luciferases, Renilla / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data; D000072183:Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics; D014162:Transfection; D015854:Up-Regulation
chemical_list: C534424:MIRN184 microRNA, human; D035683:MicroRNAs; D049410:Luciferases, Renilla; D049409:Luciferases, Firefly; D010744:Phosphoric Monoester Hydrolases; C098706:INPPL1 protein, human; D000072183:Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases; D053148:Caspase 3; D053179:Caspase 7
keywords: Antiapoptosis; HCC; INPPL1; MiR-184; Proliferation