title: Comparison of commercial hybridization and automated transcription-mediated amplification modalities for detection of high-risk human papillomavirus nucleic acid.
abstract: Assessment of 4,056 cytology collections by Cervista HPV HR and APTIMA HPV yielded 88.7% concordance, with increased detection by Cervista in patients with atypical squamous cells of undetermined significance (ASC-US) and patients negative for intraepithelial lesions and malignancy (NILM) (P ≤ 0.02). Both assays detected ≥91.7% of cervical intraepithelial neoplasia grade 2+ (CIN2+) lesions. A total of 262 specimens demonstrated luminescence within all three Cervista oligonucleotide mixtures (triple positive). APTIMA HPV and PCR-based microarray confirmed triple-positive results at rates of 7.3% and 5.9%, respectively.
authors.affiliation: Wheaton Franciscan Laboratory, Wauwatosa, Wisconsin, USA.
authors.forename: Erik
authors.initials: E
authors.lastname: Munson
authors.forename: Lynn
authors.initials: L
authors.lastname: Kroeger
authors.forename: Sandra
authors.initials: S
authors.lastname: Balzer
authors.forename: Robert
authors.initials: R
authors.lastname: Amrhein
authors.forename: Kimber L
authors.initials: KL
authors.lastname: Munson
authors.forename: Maureen
authors.initials: M
authors.lastname: Napierala
authors.forename: Richard
authors.initials: R
authors.lastname: Hudspeth
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Dillon
mesh_terms: D000328:Adult; D060085:Coinfection / Q000821:virology; D005260:Female; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology*; D014481:United States; D014773:Virology / Q000379:methods*; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000821:virology