title: Global transcriptome analysis of AtPAP2--overexpressing Arabidopsis thaliana with elevated ATP.
abstract: BACKGROUND
  AtPAP2 is a purple acid phosphatase that is targeted to both chloroplasts and mitochondria. Over-expression (OE) lines of AtPAP2 grew faster, produced more seeds, and contained higher leaf sucrose and glucose contents. The present study aimed to determine how high energy status affects leaf and root transcriptomes.
  
  
  RESULTS
  ATP and ADP levels in the OE lines are 30-50% and 20-50% higher than in the wild-type (WT) plants. Global transcriptome analyses indicated that transcriptional regulation does play a role in sucrose and starch metabolism, nitrogen, potassium and iron uptake, amino acids and secondary metabolites metabolism when there is an ample supply of energy. While the transcript abundance of genes encoding protein components of photosystem I (PS I), photosystem II (PS II) and light harvesting complex I (LHCI) were unaltered, changes in transcript abundance for genes encoding proteins of LHCII are significant. The gene expressions of most enzymes of the Calvin cycle, glycolysis and the tricarboxylic acid (TCA) cycle were unaltered, as these enzymes are known to be regulated by light/redox status or allosteric modulation by the products (e.g. citrate, ATP/ADP ratio), but not at the level of transcription.
  
  
  CONCLUSIONS
  AtPAP2 overexpression resulted in a widespread reprogramming of the transcriptome in the transgenic plants, which is characterized by changes in the carbon, nitrogen, potassium, and iron metabolism. The fast-growing AtPAP2 OE lines provide an interesting tool for studying the regulation of energy system in plant.
authors.forename: Feng
authors.initials: F
authors.lastname: Sun
authors.forename: Chao
authors.initials: C
authors.lastname: Liang
authors.forename: James
authors.initials: J
authors.lastname: Whelan
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: School of Biological Sciences, the University of Hong Kong, Pokfulam, Hong Kong, China. bllim@hku.hk.
authors.forename: Boon Leong
authors.initials: BL
authors.lastname: Lim
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004734:Energy Metabolism / Q000235:genetics; D020869:Gene Expression Profiling; D045342:Light-Harvesting Protein Complexes / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D053719:Tandem Mass Spectrometry; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D045342:Light-Harvesting Protein Complexes; C514560:PAP2 protein, Arabidopsis; D014157:Transcription Factors; D000255:Adenosine Triphosphate; D010088:Oxidoreductases