title: Graphene based soft nanoreactors for facile "one-step" glycan enrichment and derivatization for MALDI-TOF-MS analysis.
abstract: Protein glycosylation is involved in the control of many important biological processes and structural alterations of the N-linked glycans are correlated with various kinds of disease. High-throughput N-glycan profiling is a key technique for elucidating the functions of glycans in biological process and disease development as well as discovering new diagnostic biomarkers. However, the low abundance of glycans existing in living organism, the competition/suppression effect of other highly abundant biological molecules and the inherent lack of alkalinity and hydrophobicity of glycans leads to particularly poor detection sensitivity in MS analysis. Here, we demonstrated the first "one-step" approach for highly efficient glycan enrichment and derivatization using reduced graphene oxide as nanoreactors and 1-pyrenebutyric hydrazide for glycan capture and derivatization, which resulted in a 33-fold increase in the glycan detection sensitivity in MALDI-TOF-MS and the identification of 48N-glycoforms from human plasma.
authors.affiliation: School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, PR China; National Center for Protein Sciences Beijing, State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, PR China.
authors.forename: Haihong
authors.initials: H
authors.lastname: Bai
authors.forename: Yiting
authors.initials: Y
authors.lastname: Pan
authors.forename: Wei
authors.initials: W
authors.lastname: Tong
authors.forename: Wanjun
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Ren
authors.forename: Fang
authors.initials: F
authors.lastname: Tian
authors.forename: Bo
authors.initials: B
authors.lastname: Peng
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Yangjun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yulin
authors.initials: Y
authors.lastname: Deng
authors.forename: Weijie
authors.initials: W
authors.lastname: Qin
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Qian
mesh_terms: D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism; D002240:Carbohydrate Sequence; D006031:Glycosylation; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006834:Hydrazines / Q000737:chemistry; D008969:Molecular Sequence Data; D049329:Nanostructures / Q000737:chemistry; D010087:Oxides; D011134:Polysaccharides / Q000097:blood* / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods
chemical_list: D001798:Blood Proteins; D006834:Hydrazines; D010087:Oxides; D011134:Polysaccharides; D011721:Pyrenes; C002218:1-pyrenebutyrate; D006108:Graphite
keywords: 1-Pyrenebutyric hydrazide; Derivatization; Desalting; Enrichment; Glycan; Reduced graphene oxide