title: Cytokinin-enhanced accumulation of indole alkaloids in Catharanthus roseus cell cultures - The factors affecting the cytokinin response.
abstract: Cytokinins were found to stimulate the alkaloid synthesis induced by removing auxin from the medium of a cell line of Catharanthus roseus. Diluting the mineral salts of the culture medium decreased the alkaloid production but increased the "sensitivity" of the cells. Addition of high levels of Ca(2+), Mg(2+) or Sr(2+) to B5 media in which the mineral salts were diluted to 5-40%, increased the alkaloid production. The latter effect is related only partially to enhanced osmotic potential.
authors.affiliation: Laboratoire de Biologie Cellulaire et Biochimie Végetale, Faculté de Pharmacie, Université de Tours, 2bis Bd Tonnellé, F-37042, Tours, France.
authors.forename: A
authors.initials: A
authors.lastname: Decendit
authors.forename: D
authors.initials: D
authors.lastname: Liu
authors.forename: L
authors.initials: L
authors.lastname: Ouelhazi
authors.forename: P
authors.initials: P
authors.lastname: Doireau
authors.forename: J M
authors.initials: JM
authors.lastname: Mérillon
authors.forename: M
authors.initials: M
authors.lastname: Rideau