title: Examination of native chemical ligation using peptidyl prolyl thioesters.
abstract: Peptidyl prolyl thioesters, which have been regarded as too unreactive for practical use in native chemical ligations (NCLs), were proven to be versatile reagents in peptide coupling. Tuning the reactivity of prolyl thioesters enabled a one-pot/sequential NCL reaction based on kinetically controlled ligation.
authors.affiliation: Institute of Health Biosciences and Graduate School of Pharmaceutical Sciences, The University of Tokushima, 1-78-1 Shomachi, Tokushima 770-8505, Japan. aotaka@tokushima-u.ac.jp.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nakamura
authors.forename: Akira
authors.initials: A
authors.lastname: Shigenaga
authors.forename: Kohei
authors.initials: K
authors.lastname: Sato
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tsuda
authors.forename: Ken
authors.initials: K
authors.lastname: Sakamoto
authors.forename: Akira
authors.initials: A
authors.lastname: Otaka
mesh_terms: D000595:Amino Acid Sequence; D004952:Esters; D007700:Kinetics; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D004952:Esters; D010455:Peptides; D013438:Sulfhydryl Compounds