title: Resveratrol--a potential inhibitor of biofilm formation in Vibrio cholerae.
abstract: Resveratrol, a phytochemical commonly found in the skin of grapes and berries, was tested for its biofilm inhibitory activity against Vibrio cholerae. Biofilm inhibition was assessed using crystal violet assay. MTT assay was performed to check the viability of the treated bacterial cells and the biofilm architecture was analysed using confocal laser scanning microscopy. The possible target of the compound was determined by docking analysis. Results showed that subinhibitory concentrations of the compound could significantly inhibit biofilm formation in V. cholerae in a concentration-dependent manner. AphB was found to be the putative target of resveratrol using docking analysis. The results generated in this study proved that resveratrol is a potent biofilm inhibitor of V. cholerae and can be used as a novel therapeutic agent against cholera. To our knowledge, this is the first report of resveratrol showing antibiofilm activity against V. cholerae.
authors.affiliation: Cholera and Environmental Microbiology Laboratory, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram 695 014, Kerala, India.
authors.forename: Nimmy
authors.initials: N
authors.lastname: Augustine
authors.affiliation: Defence Research & Development Establishment, Gwalior 474 002, Madhya Pradesh, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Goel
authors.affiliation: Distributed Information Sub-Centre, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram 695 014, Kerala, India.
authors.forename: K C
authors.initials: KC
authors.lastname: Sivakumar
authors.affiliation: Mycobacterium Research Group, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram 695 014, Kerala, India.
authors.forename: R Ajay
authors.initials: RA
authors.lastname: Kumar
authors.affiliation: Cholera and Environmental Microbiology Laboratory, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram 695 014, Kerala, India. Electronic address: sabu@rgcb.res.in.
authors.forename: Sabu
authors.initials: S
authors.lastname: Thomas
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D062105:Molecular Docking Simulation; D010936:Plant Extracts / Q000494:pharmacology*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology* / Q000627:therapeutic use; D015534:Trans-Activators / Q000378:metabolism; D014735:Vibrio Infections / Q000188:drug therapy / Q000382:microbiology*; D014734:Vibrio cholerae / Q000378:metabolism / Q000472:pathogenicity*; D027843:Vitis / Q000737:chemistry*
chemical_list: C120327:AphB protein, Vibrio cholerae; D001426:Bacterial Proteins; D010936:Plant Extracts; D013267:Stilbenes; D015534:Trans-Activators; D000077185:Resveratrol
keywords: AphB protein; Biofilm inhibition; Molecular docking; Resveratrol; V. cholerae