title: Venom present in sea anemone (Heteractis magnifica) induces apoptosis in non-small-cell lung cancer A549 cells through activation of mitochondria-mediated pathway.
abstract: Lung cancer is a major cause of cancer deaths throughout the world and the complexity of apoptosis resistance in lung cancer is apparent. Venom from Heteractis magnifica caused dose-dependent decreases in survival of the human non-small-cell lung cancer cell line, as determined by the MTT and Crystal Violet assays. The H. magnifica venom induced cell cycle arrest and induced apoptosis of A549 cells, as confirmed by annexin V/propidium iodide staining. The venom-induced apoptosis in A549 cells was characterized by cleavage of caspase-3 and a reduction in the mitochondrial membrane potential. Interestingly, crude extracts from H. magnifica had less effect on the survival of non-cancer cell lines. In the non-cancer cells, the mechanism via which cell death occurred was through necrosis not apoptosis. These findings are important for future work using H. magnifica venom for pharmaceutical development to treat human lung cancer.
authors.affiliation: Department of Medical Biotechnology, School of Medicine, Flinders University of South Australia, Bedford Park, SA, 5042, Australia, rame0010@flinders.edu.au.
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Ramezanpour
authors.forename: Karen Burke
authors.initials: KB
authors.lastname: da Silva
authors.forename: Barbara J S
authors.initials: BJ
authors.lastname: Sanderson
mesh_terms: D031883:Anemone; D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects*; D005562:Formazans / Q000378:metabolism; D005840:Gentian Violet / Q000378:metabolism; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects*; D013194:Staining and Labeling; D013778:Tetrazolium Salts / Q000378:metabolism; D014688:Venoms / Q000633:toxicity*
chemical_list: D005562:Formazans; D013778:Tetrazolium Salts; D014688:Venoms; C079782:MTT formazan; D005840:Gentian Violet