title: High frequency of tetraploidy in Agrobacterium-mediated transformants regenerated from tuber discs of diploid potato lines.
abstract: Variations in the ploidy level of 69 transgenic potato (Solanum tuberosum L.) plants regenerated from the tuber discs of 17 diploid lines were studied: 24 plants (35%) were diploid, the other 45 plants (65%) were tetraploid. Seventy-eight control regenerants obtained without Agrobacterium inoculation showed a relatively low tendency to tetraploidization (35%). The results obtained suggested that chromosome doubling occurred frequently in diploid potato lines during the tissue culture process for regeneration. Putative somaclonal changes in in vitro-formed tuber proteins were detected in three out of six transformants by electrophoretic analysis.
authors.affiliation: Department of Cell Biology, National Institute of Agrobiological Resources, Kannondai, Tsukuba, 305, Ibaraki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Imai
authors.forename: R
authors.initials: R
authors.lastname: Aida
authors.forename: T
authors.initials: T
authors.lastname: Ishige