title: Assembly of pyrene-modified DNA/RNA duplexes incorporating a G-rich single strand region.
abstract: The structural properties of a DNA/RNA duplex having a pyrene residue at the 5' end of DNA and a G-rich single strand region at the 3' end of RNA were studied in detail. Fluorescence and ultracentrifugation analyses indicated the formation of a complex containing four DNA/RNA duplexes, which required a pyrene residue, G-rich sequence, RNA-type backbone, and high salt concentration.
authors.affiliation: Department of Life Science, Tokyo Institute of Technology, 4259 Midori-ku, Nagatsuta, Yokohama 226-8501, Japan. Electronic address: kseio@bio.titech.ac.jp.
authors.forename: Kohji
authors.initials: K
authors.lastname: Seio
authors.forename: Munefumi
authors.initials: M
authors.lastname: Tokugawa
authors.forename: Hirosuke
authors.initials: H
authors.lastname: Tsunoda
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ohkubo
authors.forename: Fumio
authors.initials: F
authors.lastname: Arisaka
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sekine
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D054856:G-Quadruplexes; D006147:Guanine / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D011721:Pyrenes / Q000737:chemistry*; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D014461:Ultracentrifugation
chemical_list: D011721:Pyrenes; D006147:Guanine; D012313:RNA; D004247:DNA; C030984:pyrene
keywords: G-quadruplex; Pyrene excimer; Sedimentation analysis