title: Study for collecting background data on Wistar Hannover [Crl:WI(Han)] rats in general toxicity studies--comparative data to Sprague Dawley rats.
abstract: The purpose of the present study was to collect background data from repeated dose toxicity studies in Wistar Hannover [Crl:WI(Han)] (hereafter Wistar Han) rats with dosing periods of 4, 13 and 26 weeks from four safety research facilities of pharmaceutical companies and contract research organizations participating in the International Genetic Standardization (IGS) rat forum supported by Charles River Laboratories Japan, Inc. The data from Wistar Han rats were compared with those from Sprague Dawley Crl:CD(SD) rats. In addition, the effects of restricted feeding of SD rats were also investigated by one facility. As a result, body weights and food consumption in Wistar Han rats were lower than those of SD rats. White blood cell (WBC), neutrophil, lymphocyte, monocyte and eosinophil counts were almost half of those noted for SD rats and platelet counts were almost 20% less than those in SD rats. Minimal strain differences were noted in several biochemical parameters including aspartate aminotransferase (AST), alanine aminotransferase, total cholesterol, triglyceride and phospholipids, and in thymus, ovary and testis weights. Ophthalmologic or histopathologic examinations revealed a higher incidence of corneal opacities or corneal mineralization in Wistar Han rats. Restricted feeding of SD rats resulted in intermediate values for body weights and food consumption between the ad libitum fed SD and Wistar Han rats, and WBC and AST were lower than those in the ad libitum fed SD rats. Based on these results, some strain differences might be ascribable to reduced food consumption and associated body weight changes in Wistar Han rats.
authors.affiliation: Kawashima Drug Safety, Global Drug Safety, Biopharmaceutical Assessment Core Function Unit, Eisai Co., Ltd., Present address; Preclinical Safety Research Laboratories, Sunplanet Co., Ltd.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Hayakawa
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Mimura
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Tachibana
authors.forename: Mami
authors.initials: M
authors.lastname: Furuya
authors.forename: Terutaka
authors.initials: T
authors.lastname: Kodama
authors.forename: Toyohiko
authors.initials: T
authors.lastname: Aoki
authors.forename: Satoru
authors.initials: S
authors.lastname: Hosokawa
authors.forename: Motoko
authors.initials: M
authors.lastname: Fukui
authors.forename: Seiji
authors.initials: S
authors.lastname: Shibata
authors.forename: Masanao
authors.initials: M
authors.lastname: Yoshida
authors.forename: Takeshi
authors.initials: T
authors.lastname: Masuyama
authors.forename: Takahiro
authors.initials: T
authors.lastname: Narita
authors.forename: Makiko
authors.initials: M
authors.lastname: Kuwagata
authors.forename: Shigeru
authors.initials: S
authors.lastname: Hisada
authors.forename: Eiji
authors.initials: E
authors.lastname: Maki
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001772:Blood Cell Count; D001835:Body Weight; D003318:Corneal Opacity / Q000453:epidemiology; D004435:Eating; D005260:Female; D008055:Lipids / Q000097:blood; D008297:Male; D023421:Models, Animal; D009929:Organ Size; D010053:Ovary; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013737:Testis; D013950:Thymus Gland; D018675:Toxicity Tests; D014116:Toxicology / Q000379:methods*
chemical_list: D008055:Lipids; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase