title: Biotransformation of cinnamyl alcohol to rosavins by non-transformed wild type and hairy root cultures of Rhodiola kirilowii.
abstract: Non-transformed wild type (NTWT) and hairy root cultures of Rhodiola kirilowii were grown in medium supplemented with 2.5 mM cinnamyl alcohol as a precursor and/or sucrose (1 %) on the day of inoculation or on the 14th day of culture. Rosarin, rosavin, and rosin were produced by NTWT root culture but only rosarin and rosavin by hairy roots. Approximately 80 and 95 % of the glycosides were released into the medium for NTWT and hairy root cultures, respectively. The highest rosavin yield, 505 ± 106 mg/l, was in hairy root culture when cinnamyl alcohol was applied on the day of inoculation with the addition of sucrose on the 14th day of culture. For rosin production, supplementation with cinnamyl alcohol alone on day 14 was more favourable with the highest amount 74 ± 10 mg/l in NTWT root culture. Only traces of rosarin were detected.
authors.affiliation: Department of Biology and Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1 St., 02-097, Warsaw, Poland, mgrech@wum.edu.pl.
authors.forename: Marta
authors.initials: M
authors.lastname: Grech-Baran
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Sykłowska-Baranek
authors.forename: Anna
authors.initials: A
authors.lastname: Krajewska-Patan
authors.forename: Anna
authors.initials: A
authors.lastname: Wyrwał
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Pietrosiuk
mesh_terms: D001711:Biotransformation; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D004187:Disaccharides / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D020005:Propanols / Q000378:metabolism*; D012116:Resins, Plant / Q000378:metabolism*; D029771:Rhodiola / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism
chemical_list: D003470:Culture Media; D004187:Disaccharides; D020005:Propanols; D012116:Resins, Plant; C529148:rosavin; D013395:Sucrose; C013893:rosin; C529149:rosarin; C020722:cinnamyl alcohol