title: Long-chain free fatty acid profiling analysis by liquid chromatography-mass spectrometry in mouse treated with peroxisome proliferator-activated receptor α agonist.
abstract: A change in the free fatty acid (FFA) profile reflects an alteration in the lipid metabolism of peripheral tissue. A high-throughput quantitative analysis method for individual FFAs therefore needs to be established. We report here an optimized LC-MS assay for a high-throughput and high-sensitivity analysis of the 10 major long-chain FFAs in mouse plasma and liver. This assay enables quantification of individual FFAs by using trace amounts of samples (2 µL of plasma and 10 mg of liver tissue). We apply this method to analyze the FFA profile of plasma and liver samples from an obese mouse model treated with bezafibrate, the peroxisome proliferator-activated receptor α (PPARα) agonist, and show a change in the FFA profile, particularly in the palmitoleic and oleic acid contents. This assay is useful for quantifying individual FFAs and helpful for monitoring the condition of lipid metabolism.
authors.affiliation: Laboratory of Molecular Functions of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University.
authors.forename: Haruya
authors.initials: H
authors.lastname: Takahashi
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Suzuki
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Suda
authors.forename: Yota
authors.initials: Y
authors.lastname: Yamazaki
authors.forename: Akihiro
authors.initials: A
authors.lastname: Takino
authors.forename: Young-Il
authors.initials: YI
authors.lastname: Kim
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Goto
authors.forename: Yoko
authors.initials: Y
authors.lastname: Iijima
authors.forename: Koh
authors.initials: K
authors.lastname: Aoki
authors.forename: Daisuke
authors.initials: D
authors.lastname: Shibata
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Takahashi
authors.forename: Teruo
authors.initials: T
authors.lastname: Kawada
mesh_terms: D000818:Animals; D001629:Bezafibrate / Q000494:pharmacology*; D002853:Chromatography, Liquid; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D015870:Gene Expression; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008820:Mice, Obese; D009765:Obesity / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D047493:PPAR alpha / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism
chemical_list: D005230:Fatty Acids, Nonesterified; D000960:Hypolipidemic Agents; D047493:PPAR alpha; C490333:Scd1 protein, mouse; D013230:Stearoyl-CoA Desaturase; D001629:Bezafibrate