title: Statistical medium optimization of an alkaline protease from Pseudomonas aeruginosa MTCC 10501, its characterization and application in leather processing.
abstract: Proteases are shown to have greener mode of application in leather processing for dehairing of goat skins and cow hides. Production of protease by submerged fermentation with potent activity is reported using a new isolate P. aeruginosa MTCC 10501. The production parameters were optimized by statistical methods such as Plackett-Burman and response surface methodology. The optimized production medium contained (g/L); tryptone, 2.5; yeast extract, 3.0; skim milk 30.0; dextrose 1.0; inoculum concentration 4%: initial pH 6.0; incubation temperature 30 degrees C and optimum production at 48 h with protease activity of 7.6 U/mL. The protease had the following characteristics: pH optima, 9.0; temperature optima 50 degrees C; pH stability between 5.0-10.0 and temperature stability between 10-40 degrees C. The protease was observed to have high potential for dehairing of goat skins in the pre- tanning process comparable to that of the chemical process as evidenced by histology. The method offers cleaner processing using enzyme only instead of toxic chemicals in the pre-tanning process of leather manufacture.
authors.affiliation: Department of Biotechnology, CSIR-Central Leather Research Institute (CLIR), Adyar Chennai 600 020, India. boopathyclri@gmail.com
authors.forename: Naidu Ramachandra
authors.initials: NR
authors.lastname: Boopathy
authors.forename: Devadas
authors.initials: D
authors.lastname: Indhuja
authors.forename: Krishnan
authors.initials: K
authors.lastname: Srinivasan
authors.forename: Mani
authors.initials: M
authors.lastname: Uthirappan
authors.forename: Rishikesh
authors.initials: R
authors.lastname: Gupta
authors.forename: Kamini Numbi
authors.initials: KN
authors.lastname: Ramudu
authors.forename: Rose
authors.initials: R
authors.lastname: Chellan
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis*; D003470:Culture Media; D010450:Endopeptidases / Q000096:biosynthesis*; D006041:Goats; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods*; D007221:Industry; D015233:Models, Statistical; D010461:Peptones / Q000737:chemistry; D011312:Pressure; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D013633:Tanning; D013696:Temperature; D015003:Yeasts / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D010461:Peptones; C001830:tryptones; D010450:Endopeptidases; C087822:alkaline protease