title: Click aspartic acid as H ILIC SPE material for selective enrichment of N-linked glycopeptides.
abstract: A novel hydrophilic interaction chromatography (HILIC) material was developed via clicking aspartic acid onto silica gel (termed as Click AA). The material demonstrated highly hydrophilic property and was used as solid phase extraction sorbent for selective enrichment of glycopeptides. By optimization of extraction conditions, Click AA exhibited high selectivity to glycopeptides with various peptide length and different types of glycans, which was much superior to similar commercial products. The application of Click AA to simulated proteomic samples further proved its specialty toward glycopeptides.
authors.affiliation: Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian 116023, China.
authors.forename: Xiuling
authors.initials: X
authors.lastname: Li
authors.forename: Guobin
authors.initials: G
authors.lastname: Shen
authors.forename: FeiFang
authors.initials: F
authors.lastname: Zhang
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Yang
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D001224:Aspartic Acid / Q000737:chemistry*; D006020:Glycopeptides / Q000302:isolation & purification*; D006801:Humans; D058428:Silica Gel / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D006020:Glycopeptides; D001224:Aspartic Acid; D058428:Silica Gel
keywords: Aspartic acid; Click chemistry; Glycopeptides; Hydrophilic interaction chromatography; Solid phase extraction