title: Scope extension validation protocol: inclusion of analytes and matrices in an LC-MS/MS sulfonamide residues method.
abstract: Validation is a required process for analytical methods. However, scope extension, i.e. inclusion of more analytes, other matrices and/or minor changes in extraction procedures, can be achieved without a full validation protocol, which requires time and is laborious to the laboratory. This paper presents a simple and rugged protocol for validation in the case of extension of scope. Based on a previously reported method for analysis of sulfonamide residues using LC-MS/MS, inclusion of more analytes, metabolites, matrices and optimisation for the extraction procedure are presented in detail. Initially, the method was applied only to liver samples. In this work, milk, eggs and feed were also added to the scope. Several case-specific validation protocols are proposed for extension of scope.
authors.affiliation: a Instituto de Química , Universidade Federal do Rio Grande do Sul , Porto Alegre , RS , Brazil.
authors.forename: Rodrigo Barcellos
authors.initials: RB
authors.lastname: Hoff
authors.forename: Fabiano
authors.initials: F
authors.lastname: Barreto
authors.forename: Jéssica
authors.initials: J
authors.lastname: Melo
authors.forename: Magda Targa
authors.initials: MT
authors.lastname: Martins
authors.forename: Tânia Mara
authors.initials: TM
authors.lastname: Pizzolato
authors.forename: Maria do Carmo Ruaro
authors.initials: Mdo C
authors.lastname: Peralba
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis; D002853:Chromatography, Liquid; D004350:Drug Residues / Q000032:analysis*; D004531:Eggs / Q000032:analysis; D005506:Food Contamination / Q000032:analysis*; D059022:Food Safety; D064088:Hazard Analysis and Critical Control Points / Q000379:methods; D006801:Humans; D008099:Liver / Q000737:chemistry; D008892:Milk / Q000737:chemistry; D013449:Sulfonamides / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D019155:Veterinary Drugs / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D013449:Sulfonamides; D019155:Veterinary Drugs