title: Immunosuppressive CD71+ erythroid cells compromise neonatal host defence against infection.
abstract: Newborn infants are highly susceptible to infection. This defect in host defence has generally been ascribed to the immaturity of neonatal immune cells; however, the degree of hyporesponsiveness is highly variable and depends on the stimulation conditions. These discordant responses illustrate the need for a more unified explanation for why immunity is compromised in neonates. Here we show that physiologically enriched CD71(+) erythroid cells in neonatal mice and human cord blood have distinctive immunosuppressive properties. The production of innate immune protective cytokines by adult cells is diminished after transfer to neonatal mice or after co-culture with neonatal splenocytes. Neonatal CD71(+) cells express the enzyme arginase-2, and arginase activity is essential for the immunosuppressive properties of these cells because molecular inhibition of this enzyme or supplementation with L-arginine overrides immunosuppression. In addition, the ablation of CD71(+) cells in neonatal mice, or the decline in number of these cells as postnatal development progresses parallels the loss of suppression, and restored resistance to the perinatal pathogens Listeria monocytogenes and Escherichia coli. However, CD71(+) cell-mediated susceptibility to infection is counterbalanced by CD71(+) cell-mediated protection against aberrant immune cell activation in the intestine, where colonization with commensal microorganisms occurs swiftly after parturition. Conversely, circumventing such colonization by using antimicrobials or gnotobiotic germ-free mice overrides these protective benefits. Thus, CD71(+) cells quench the excessive inflammation induced by abrupt colonization with commensal microorganisms after parturition. This finding challenges the idea that the susceptibility of neonates to infection reflects immune-cell-intrinsic defects and instead highlights processes that are developmentally more essential and inadvertently mitigate innate immune protection. We anticipate that these results will spark renewed investigation into the need for immunosuppression in neonates, as well as improved strategies for augmenting host defence in this vulnerable population.
authors.affiliation: Division of Infectious Diseases and Perinatal Institute, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, Ohio 45229, USA.
authors.forename: Shokrollah
authors.initials: S
authors.lastname: Elahi
authors.forename: James M
authors.initials: JM
authors.lastname: Ertelt
authors.forename: Jeremy M
authors.initials: JM
authors.lastname: Kinder
authors.forename: Tony T
authors.initials: TT
authors.lastname: Jiang
authors.forename: Xuzhe
authors.initials: X
authors.lastname: Zhang
authors.forename: Lijun
authors.initials: L
authors.lastname: Xin
authors.forename: Vandana
authors.initials: V
authors.lastname: Chaturvedi
authors.forename: Beverly S
authors.initials: BS
authors.lastname: Strong
authors.forename: Joseph E
authors.initials: JE
authors.lastname: Qualls
authors.forename: Kris A
authors.initials: KA
authors.lastname: Steinbrecher
authors.forename: Theodosia A
authors.initials: TA
authors.lastname: Kalfa
authors.forename: Aimen F
authors.initials: AF
authors.lastname: Shaaban
authors.forename: Sing Sing
authors.initials: SS
authors.lastname: Way
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015703:Antigens, CD / Q000378:metabolism*; D001119:Arginase / Q000235:genetics / Q000378:metabolism; D004198:Disease Susceptibility / Q000276:immunology; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D041905:Erythroid Cells / Q000201:enzymology / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology; D004927:Escherichia coli Infections / Q000276:immunology*; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D007108:Immune Tolerance / Q000187:drug effects / Q000235:genetics / Q000276:immunology*; D008089:Listeria monocytogenes / Q000276:immunology; D008088:Listeriosis / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011990:Receptors, Transferrin / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C068126:CD71 antigen; D004791:Enzyme Inhibitors; D011990:Receptors, Transferrin; D014409:Tumor Necrosis Factor-alpha; D001119:Arginase