title: Arsenic trioxide induces apoptosis in B-cell chronic lymphocytic leukemic cells through down-regulation of survivin via the p53-dependent signaling pathway.
abstract: Arsenic trioxide (As2O3) can induce apoptosis in many tumors. However, the associated mechanisms are not clearly understood. We found that As2O3 significantly inhibited the proliferation of WSU-CLL cells and induced apoptosis in dose- and time-dependent manners. WSU-CLL cells treated with 2μM As2O3 showed survivin down-regulation and p53 up-regulation. Survivin siRNA combined with As2O3 further inhibited the proliferation of WSU-CLL cells. p53 inhibition by siRNA prevented the down-regulation of survivin by As2O3 and prevented the As2O3-induced cytotoxicity of WSU-CLL cells. These results suggest that As2O3 may be of therapeutic value for chronic lymphocytic leukemia.
authors.affiliation: Peking University People's Hospital, Peking University Institute of Hematology, Beijing 100044, China. Electronic address: zhangxh100@sina.com.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Zhang
authors.forename: Ru
authors.initials: R
authors.lastname: Feng
authors.forename: Meng
authors.initials: M
authors.lastname: Lv
authors.forename: Qian
authors.initials: Q
authors.lastname: Jiang
authors.forename: Hong-Hu
authors.initials: HH
authors.lastname: Zhu
authors.forename: Ya-Zhen
authors.initials: YZ
authors.lastname: Qing
authors.forename: Jia-Ling
authors.initials: JL
authors.lastname: Bao
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Huang
authors.forename: X Long
authors.initials: XL
authors.lastname: Zheng
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D015536:Down-Regulation / Q000187:drug effects; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000473:pathology*; D010087:Oxides / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D000077022:Survivin; D016159:Tumor Suppressor Protein p53 / Q000502:physiology*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D001152:Arsenicals; C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D010087:Oxides; D000077022:Survivin; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D000077237:Arsenic Trioxide
keywords: Apoptosis; Arsenic trioxide; P53 and CLL; Survivin; WSU-CLL