title: Knockdown of p66Shc by siRNA injection rescues arsenite-induced developmental retardation in mouse preimplantation embryos.
abstract: Two-cell arrest plays a principal role in the elevated levels of embryo loss during the first week of development in mouse. Previously, we have shown that arsenic can apparently induce 2-cell arrest in mouse preimplantation embryo and the expression of oxidative stress adaptor protein p66(Shc) is up-regulated in this process. In the present study, we demonstrated that microinjection of p66(Shc) siRNA into the pronucleus of zygotes resulted in a markedly decrease in both mRNA and protein levels of p66(Shc). The arsenite-induced 2-cell arrests, along with a reduction in the levels of reactive oxygen species (ROS), were significantly inhibited and the number of embryos developing to morula stage concurrently increased upon p66(shc) siRNA microinjection. These findings indicate that knockdown of p66(shc) improves the developmental competence of arsenite-exposed embryos in vitro by increasing the resistance to oxidative stress. In addition, we highlight the utility of single-embryo analysis in preimplantation embryos.
authors.affiliation: Graduate School of Peking Union Medical College, Beijing, China; Department of Genetics, National Research Institute for Family Planning, Beijing, China; World Health Organizations Collaborative Center for Research in Human Reproduction, Beijing, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Ren
authors.affiliation: Graduate School of Peking Union Medical College, Beijing, China; Department of Genetics, National Research Institute for Family Planning, Beijing, China; World Health Organizations Collaborative Center for Research in Human Reproduction, Beijing, China.
authors.forename: Xiaoqiao
authors.initials: X
authors.lastname: Li
authors.affiliation: Graduate School of Peking Union Medical College, Beijing, China; Department of Genetics, National Research Institute for Family Planning, Beijing, China; World Health Organizations Collaborative Center for Research in Human Reproduction, Beijing, China.
authors.forename: Jinting
authors.initials: J
authors.lastname: Yan
authors.affiliation: Children's Hospital of Fudan University, Shanghai, China.
authors.forename: Guoying
authors.initials: G
authors.lastname: Huang
authors.affiliation: Graduate School of Peking Union Medical College, Beijing, China; Department of Genetics, National Research Institute for Family Planning, Beijing, China; World Health Organizations Collaborative Center for Research in Human Reproduction, Beijing, China.
authors.forename: Shiyi
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Graduate School of Peking Union Medical College, Beijing, China; Department of Genetics, National Research Institute for Family Planning, Beijing, China; World Health Organizations Collaborative Center for Research in Human Reproduction, Beijing, China.
authors.forename: Baiqing
authors.initials: B
authors.lastname: Yang
authors.affiliation: Graduate School of Peking Union Medical College, Beijing, China; Department of Genetics, National Research Institute for Family Planning, Beijing, China; World Health Organizations Collaborative Center for Research in Human Reproduction, Beijing, China. Electronic address: genetic@263.net.cn.
authors.forename: Xu
authors.initials: X
authors.lastname: Ma
authors.affiliation: Graduate School of Peking Union Medical College, Beijing, China; Department of Genetics, National Research Institute for Family Planning, Beijing, China; World Health Organizations Collaborative Center for Research in Human Reproduction, Beijing, China. Electronic address: lucailing@sina.com.
authors.forename: Cailing
authors.initials: C
authors.lastname: Lu
mesh_terms: D000818:Animals; D018053:Arsenites / Q000633:toxicity*; D001755:Blastocyst / Q000187:drug effects* / Q000378:metabolism; D047108:Embryonic Development / Q000187:drug effects / Q000502:physiology; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D018384:Oxidative Stress; D011247:Pregnancy; D034741:RNA, Small Interfering / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D055765:Shc Signaling Adaptor Proteins / Q000235:genetics* / Q000378:metabolism; D017670:Sodium Compounds / Q000633:toxicity*; D000071425:Src Homology 2 Domain-Containing, Transforming Protein 1
chemical_list: D018053:Arsenites; D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species; D055765:Shc Signaling Adaptor Proteins; C528034:Shc1 protein, mouse; D017670:Sodium Compounds; D000071425:Src Homology 2 Domain-Containing, Transforming Protein 1; C017947:sodium arsenite
keywords: Arsenite; Embryo arrest; Pronuclear microinjection; Reactive oxygen species; p66(Shc); siRNA