title: Identification of α-amylase by random and specific mutagenesis of Texcoconibacillus texcoconensis 13CCT strain isolated from extreme alkaline-saline soil of the former Lake Texcoco (Mexico).
abstract: The alkaline α-amylase produced by Texcoconibacillus texcoconensis 13CC(T) strain was identified by random mutagenesis and confirmed by directed mutagenesis. A transposon mutagenesis approach was taken to identify the gene responsible for the degradation of starch in T. texcoconensis 13CC(T) strain. The deduced amino acids of the amy gene had a 99% similarity with those of Bacillus selenitireducens MLS10 and 97% with those of Paenibacillus curdlanolyticus YK9. The enzyme showed a maximum activity of 131.1 U/mL at 37 °C and pH 9.5 to 10.5. In situ activity of the enzyme determined by polyacrylamide gel electrophoresis showed only one band with amylolytic activity. This is the first report of a bacterium isolated from the extreme alkaline-saline soil of the former Lake Texcoco (Mexico) with amylolytic activity in alkaline conditions while its potential as a source of amylases for the industry is discussed.
authors.affiliation: Laboratory of Soil Ecology, Department of Biotechnology and Bioengineering, ABACUS, CINVESTAV, Av. I.P.N. 2508, C.P. 07360, Mexico, D.F., Mexico.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Bello-López
authors.forename: Yendi E
authors.initials: YE
authors.lastname: Navarro-Noya
authors.forename: Selene
authors.initials: S
authors.lastname: Gómez-Acata
authors.forename: Zahuiti
authors.initials: Z
authors.lastname: Hernández-Montañez
authors.forename: Luc
authors.initials: L
authors.lastname: Dendooven
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D060106:Lakes; D008800:Mexico; D016296:Mutagenesis; D009154:Mutation; D054712:Salinity; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D000516:alpha-Amylases / Q000235:genetics / Q000378:metabolism*
chemical_list: D012987:Soil; D000516:alpha-Amylases