title: Moderate physical exercise reduces parasitaemia and protects colonic myenteric neurons in mice infected with Trypanosoma cruzi.
abstract: This study evaluated the influence of moderate physical exercise on the myenteric neurons in the colonic intestinal wall of mice that had been infected with Trypanosoma cruzi. Parasitology and immunological aspects of the mice were considered. Forty-day-old male Swiss mice were divided into four groups: Trained Infected (TI), Sedentary Infected (SI), Trained Control (TC), and Sedentary Control (SC). The TC and TI were subjected to a moderate physical exercise program on a treadmill for 8 weeks. Three days after finishing exercise, the TI and SI groups were inoculated with 1,300 blood trypomastigotes of the Y strain-T. cruzi. After 75 days of infection results were obtained. Kruskal-Wallis or Analyze of variance (Tukey post hoc test) at 5% level of significance was performed. Moderate physical exercise reduced both the parasite peak (day 8 of infection) and total parasitemia compared with the sedentary groups (P < 0.05). This activity also contributed to neuronal survival (P < 0.05). Exercise caused neuronal hypertrophy (P < 0.05) and an increase in the total thickness of the intestinal wall (P < 0.05). The TI group exhibited an increase in the number of intraepithelial lymphocytes (P > 0.05). In trained animals, the number of goblet cells was reduced compared with sedentary animals (P < 0.05). Physical exercise prevented the formation of inflammatory foci in the TI group (P < 0.05) and increased the synthesis of TNF-α (P < 0.05) and TGF-β (P > 0.05). The present results demonstrated the benefits of moderate physical exercise, and reaffirmed the possibility of that it may contribute to improving clinical treatment in Chagas' disease patients.
authors.affiliation: Programa de Pós-Graduação em Ciências da Saúde, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Neide M
authors.initials: NM
authors.lastname: Moreira
authors.forename: Franciele d N
authors.initials: Fd
authors.lastname: Santos
authors.forename: Max Jean d O
authors.initials: MJ
authors.lastname: Toledo
authors.forename: Solange M F d
authors.initials: SM
authors.lastname: Moraes
authors.forename: Eduardo J d A
authors.initials: EJ
authors.lastname: Araujo
authors.forename: Debora d M G
authors.initials: Dd
authors.lastname: Sant'Ana
authors.forename: Silvana M d
authors.initials: SM
authors.lastname: Araujo
mesh_terms: D000818:Animals; D002470:Cell Survival; D014355:Chagas Disease / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D003106:Colon / Q000294:innervation* / Q000473:pathology; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D009197:Myenteric Plexus / Q000473:pathology*; D009474:Neurons / Q000473:pathology; D018512:Parasitemia / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014349:Trypanosoma cruzi; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha
keywords: TGF-β; TNF-α; Trypanosoma cruzi; colon; moderate physical exercise; myenteric plexus; physical training