title: Plant regeneration from isolated microspores of Triticum aestivum.
abstract: Wheat microspores were isolated, without prior anther culture, from a range of genotypes and cultured to regenerate self-fertile plants. Microspores were isolated using a microblender and competent microspores were enriched by gradient centrifugation. The use of maltose as the sole carbohydrate in the culture medium and co-culture of microspores with wheat or barley ovaries were critical for development of microspore-derived embryos. Results were also improved when spikes were pretreated by emersion of the basal ends of detached heads in water at 25°C for 2d. This procedure leads to highly reproducible production of plants.
authors.affiliation: Sogetal Inc., 3876 Bay Center Place, 94587, Hayward, California, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Mejza
authors.forename: V
authors.initials: V
authors.lastname: Morgant
authors.forename: D E
authors.initials: DE
authors.lastname: Dibona
authors.forename: J R
authors.initials: JR
authors.lastname: Wong