title: High-resolution melting analyses for genetic variants in ARID5B and IKZF1 with childhood acute lymphoblastic leukemia susceptibility loci in Taiwan.
abstract: BACKGROUND
  Childhood acute lymphoblastic leukemia (ALL), a heterogeneous disease that includes multiple subtypes is defined by cell lineage and chromosome anomalies. Previous genome-wide association studies have reported several ARID5B and IKZF1 single nucleotide polymorphisms (SNPs) associated with the incidence of ALL. High-resolution melting (HRM) analysis is a rapid and convenient technique to detect SNPs; we thereby detected SNPs in ARID5B and IKZF1 genes.
  
  
  METHODS
  We enrolled 79 pediatric ALL patients and 80 healthy controls. Polymorphic variants of IKZF1 (rs6964823, rs4132601, and rs6944602) and ARID5B (rs7073837, rs10740055, and rs7089424) were detected by HRM, and SNPs were analyzed for association with childhood ALL.
  
  
  RESULTS
  The distribution of genotype rs7073837 in ARID5B significantly differed between ALL and controls (P=0.046), while those of IKZF1 (rs6964823, rs4132601, and rs6944602) and ARID5B (rs10740055 and rs7089424) did not. We analyzed the association for SNPs with B lineage ALL to find rs7073837 in ARID5B, conferring a higher risk for B lineage ALL (odds ratio, OR=1.70, 95% confidence interval, CI=1.01-2.87, P=0.049).
  
  
  CONCLUSIONS
  HRM is a practical method to detect SNPs in ARID5B and IKZF1 genes. We found that rs7073837 in ARID5B correlated with a risk for childhood B lineage ALL.
authors.affiliation: Graduate Institute of Clinical Medical Sciences, China Medical University, Taichung, Taiwan; Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Chien-Yu
authors.initials: CY
authors.lastname: Lin
authors.affiliation: Department of Pediatrics, National Taiwan University Hospital Hsin-Chu Branch, Hsinchu, Taiwan.
authors.forename: Meng-Ju
authors.initials: MJ
authors.lastname: Li
authors.affiliation: Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan; School of Medicine, China Medical University, Taichung, Taiwan; Epigenome Research Center, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Jan-Gowth
authors.initials: JG
authors.lastname: Chang
authors.affiliation: Department of Pediatrics, Children's Hospital, China Medical University, Taichung, Taiwan.
authors.forename: Su-Ching
authors.initials: SC
authors.lastname: Liu
authors.affiliation: Department of Pediatrics, Children's Hospital, China Medical University, Taichung, Taiwan.
authors.forename: Tefu
authors.initials: T
authors.lastname: Weng
authors.affiliation: Department of Pediatrics, Children's Hospital, China Medical University, Taichung, Taiwan.
authors.forename: Kang-Hsi
authors.initials: KH
authors.lastname: Wu
authors.affiliation: Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Shu-Fen
authors.initials: SF
authors.lastname: Yang
authors.affiliation: Department of Pediatrics, Children's Hospital, China Medical University, Taichung, Taiwan.
authors.forename: Fu-Kuei
authors.initials: FK
authors.lastname: Huang
authors.affiliation: Division of Surgery, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan; Graduate Institute of Integrated Medicine, China Medical University, Taichung, Taiwan; Department of Life Science, National Chung Hsing University, Taichung, Taiwan.
authors.forename: Wan-Yu
authors.initials: WY
authors.lastname: Lo
authors.affiliation: Department of Pediatrics, Children's Hospital, China Medical University, Taichung, Taiwan; Department of Biotechnology, Asia University, Taichung, Taiwan. Electronic address: pct@mail.cmuh.org.tw.
authors.forename: Ching-Tien
authors.initials: CT
authors.lastname: Peng
mesh_terms: D000293:Adolescent; D000483:Alleles; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D004268:DNA-Binding Proteins / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D051740:Ikaros Transcription Factor / Q000235:genetics*; D007223:Infant; D007231:Infant, Newborn; D021141:Nucleic Acid Amplification Techniques; D020641:Polymorphism, Single Nucleotide; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis* / Q000235:genetics*; D040641:Quantitative Trait Loci; D013624:Taiwan; D014157:Transcription Factors / Q000235:genetics*
chemical_list: C503805:ARID5B protein, human; D004268:DNA-Binding Proteins; C497239:IKZF1 protein, human; D014157:Transcription Factors; D051740:Ikaros Transcription Factor
keywords: ARID5B; Childhood acute lymphoblastic leukemia; High-resolution melting analyses; IKZF1; Single nucleotide polymorphisms