title: Apolipoprotein C1 regulates epiboly during gastrulation in zebrafish.
abstract: Apolipoprotein C1 (Apoc1) is associated with lipoprotein metabolism, but its physiological role during embryogenesis is largely unknown. We reveal a new function of Apoc1b, a transcript isoform of Apoc1, in epiboly during zebrafish gastrulation. Apoc1b is expressed in yolk syncytial layers and in deep cells of the ventral and lateral region of the embryos. It displays a radial gradient with high levels in the interior layer and low levels in the superficial layer. Knockdown of Apoc1b by injecting antisense morpholino (MO) caused the epiboly arrest in deep cells. Moreover, we show that the radial intercalation and the radial gradient distribution of E-cadherin are disrupted both in Apoc1b knockdown and overexpressed embryos. Therefore, Apoc1b controls epiboly via E-cadherin-mediated radial intercalation in a gradient-dependent manner.
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.forename: Wenhua
authors.initials: W
authors.lastname: Li
authors.forename: Jianfang
authors.initials: J
authors.lastname: Gui
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D053302:Apolipoprotein C-I / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D015820:Cadherins / Q000378:metabolism; D002465:Cell Movement / Q000235:genetics; D054262:Gastrulation / Q000235:genetics / Q000502:physiology*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D020033:Protein Isoforms; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D053302:Apolipoprotein C-I; D015820:Cadherins; C579542:Cdh1 protein, zebrafish; D020033:Protein Isoforms; D029961:Zebrafish Proteins