title: Nocardia kroppenstedtii sp. nov., an actinomycete isolated from a lung transplant patient with a pulmonary infection.
abstract: A novel actinomycete, strain N1286(T), isolated from a lung transplant patient with a pulmonary infection, was provisionally assigned to the genus Nocardia. The strain had chemotaxonomic and morphological properties typical of members of the genus Nocardia and formed a distinct phyletic line in the Nocardia 16S rRNA gene tree. Isolate N1286(T) was most closely related to Nocardia farcinica DSM 43665(T) (99.8% gene sequence similarity) but could be distinguished from the latter by the low level of DNA-DNA relatedness. These strains were also distinguishable on the basis of a broad range of phenotypic properties. It is concluded that strain N1286(T) represents a novel species of the genus Nocardia for which the name Nocardia kroppenstedtii sp. nov. is proposed. The type strain is N1286(T) ( = DSM 45810(T) = NCTC 13617(T)).
authors.affiliation: Faculty of Health and Life Sciences, Northumbria University, Newcastle upon Tyne, NE1 8ST, UK.|Department of Microbiology, Freeman Hospital, Newcastle upon Tyne, NE7 7DN, UK.|School of Biology, University Newcastle, Newcastle upon Tyne, NE1 7RU, UK.
authors.forename: Amanda L
authors.initials: AL
authors.lastname: Jones
authors.affiliation: Institute of Transplantation, Freeman Hospital, Newcastle upon Tyne, NE7 7DN, UK.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Fisher
authors.affiliation: Institute of Transplantation, Freeman Hospital, Newcastle upon Tyne, NE7 7DN, UK.
authors.forename: Rahul
authors.initials: R
authors.lastname: Mahida
authors.affiliation: Department of Microbiology, Freeman Hospital, Newcastle upon Tyne, NE7 7DN, UK.
authors.forename: Kate
authors.initials: K
authors.lastname: Gould
authors.affiliation: Department of Microbiology, Freeman Hospital, Newcastle upon Tyne, NE7 7DN, UK.
authors.forename: John D
authors.initials: JD
authors.lastname: Perry
authors.affiliation: Department of Microbiology, Mater Misericordiae University Hospital, Dublin 7, Ireland.
authors.forename: Margaret M
authors.initials: MM
authors.lastname: Hannan
authors.affiliation: Department of Microbiology, Mater Misericordiae University Hospital, Dublin 7, Ireland.
authors.forename: Eoin P
authors.initials: EP
authors.lastname: Judge
authors.affiliation: School of Biology, University Newcastle, Newcastle upon Tyne, NE1 7RU, UK.
authors.forename: Ros
authors.initials: R
authors.lastname: Brown
authors.affiliation: Faculty of Health and Life Sciences, Northumbria University, Newcastle upon Tyne, NE1 8ST, UK.
authors.forename: Kimberley
authors.initials: K
authors.lastname: Boagey
authors.affiliation: School of Biology, University Newcastle, Newcastle upon Tyne, NE1 7RU, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Goodfellow
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006801:Humans; D008171:Lung Diseases / Q000382:microbiology*; D016040:Lung Transplantation; D008969:Molecular Sequence Data; D009615:Nocardia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8