title: Improved plant regeneration from wheat anther and barley microspore culture using phenylacetic acid (PAA).
abstract: The effect of the auxin phenylacetic acid (PAA) on wheat anther and on barley anther/microspore culture was investigated. With PAA the induction response was not usually significantly different from controls but a significantly higher number of green plants were produced in wheat anther and barley microspore culture. For wheat anther culture 100 mg/L PAA was beneficial. For barley microspore culture the optimum levels were from 1 to 100 mg/L, depending on genotype. In barley anther culture there were no improvements using PAA. In wheat anther culture, 145 green plants/100 anthers were obtained with cultivar Veery'S', while the average response from twelve F1 hybrids in the breeding program was 332 green plants/100 anthers. At least 1000 green plants were obtained using isolated microspores from 100 anthers in barley cv. Igri. With cv. Bruce, regeneration occurred only when 100 mg/L PAA was used. The influence of PAA appears at the embryogenic phase of the culture system. The possible mechanisms by which PAA may improve regeneration are discussed.
authors.affiliation: Crop Science Department, University of Guelph, N1G 2W1, Guelph, Ontario, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Ziauddin
authors.forename: A
authors.initials: A
authors.lastname: Marsolais
authors.forename: E
authors.initials: E
authors.lastname: Simion
authors.forename: K J
authors.initials: KJ
authors.lastname: Kasha