title: Plant regeneration through somatic embryogenesis from spear callus culture of Asparagus cooperi Baker.
abstract: Somatic embryogenesis and plantlet formation were obtained from callus derived from the subapical region of spears of Asparagus cooperi Baker. Callus was obtained in Murashige and Skoog's medium supplemented with 1-naphthaleneacetic acid and kinetin. Increase in the concentration of potassium nitrate in subsequent subcultures resulted in the formation of embryos. Rapid multiplication of embryos was secured on transfer to a medium containing a different source of nitrogen and a low level (0.01 mg/1) of gibberellic acid. Media containing zeatin or gibberellic acid led to the formation of complete plantlets from embryos. Regenerated plants were cytologically and phenotypically stable.
authors.affiliation: Centre of Advanced Study, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, 700019, Calcutta, India.
authors.forename: B
authors.initials: B
authors.lastname: Ghosh
authors.forename: S
authors.initials: S
authors.lastname: Sen