title: A label-free luminescent switch-on assay for ATP using a G-quadruplex-selective iridium(III) complex.
abstract: We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5'-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 µM.
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
authors.forename: Ka-Ho
authors.initials: KH
authors.lastname: Leung
authors.forename: Lihua
authors.initials: L
authors.lastname: Lu
authors.forename: Modi
authors.initials: M
authors.lastname: Wang
authors.forename: Tsun-Yin
authors.initials: TY
authors.lastname: Mak
authors.forename: Daniel Shiu-Hin
authors.initials: DS
authors.lastname: Chan
authors.forename: Fung-Kit
authors.initials: FK
authors.lastname: Tang
authors.forename: Chung-Hang
authors.initials: CH
authors.lastname: Leung
authors.forename: Hiu-Yee
authors.initials: HY
authors.lastname: Kwan
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Yu
authors.forename: Dik-Lung
authors.initials: DL
authors.lastname: Ma
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry; D045744:Cell Line, Tumor; D056831:Coordination Complexes / Q000737:chemistry*; D054856:G-Quadruplexes; D007495:Iridium / Q000737:chemistry*; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods; D051379:Mice; D015394:Molecular Structure; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
chemical_list: D052157:Aptamers, Nucleotide; D056831:Coordination Complexes; D007495:Iridium; D000255:Adenosine Triphosphate