title: Prohibitin attenuates oxidative stress and extracellular matrix accumulation in renal interstitial fibrosis disease.
abstract: Prohibitin is an evolutionary conserved and pleiotropic protein that has been implicated in various cellular functions, including proliferation, tumour suppression, apoptosis, transcription, and mitochondrial protein folding. Both prohibitin over- and under-expression have been implicated in various diseases and cell types. We recently demonstrated that prohibitin down-regulation results in increased renal interstitial fibrosis (RIF). Here we investigated the role of oxidative stress and prohibitin expression in RIF in unilateral ureteral obstructed rats. Lentivirus-based delivery vectors were used to knockdown or over-express prohibitin. Our results show that increased prohibitin expression was negatively correlated with the RIF index, reactive oxygen species, malon dialdehyde, transforming growth factor β1, collagen IV, fibronectin, and cell apoptosis index. In conclusion, we postulate that prohibitin acts as a positive regulator of mechanisms that counteract oxidative stress and extracellular matrix accumulation and therefore has an antioxidative effect.
authors.affiliation: Department of Pediatric Nephrology, the First Affiliated Hospital of GuangXi Medical University, NanNing, China ; Department of Nephrology, the Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
authors.forename: Tian-Biao
authors.initials: TB
authors.lastname: Zhou
authors.forename: Yuan-Han
authors.initials: YH
authors.lastname: Qin
authors.forename: Feng-Ying
authors.initials: FY
authors.lastname: Lei
authors.forename: Wei-Fang
authors.initials: WF
authors.lastname: Huang
authors.forename: Gregor P C
authors.initials: GP
authors.lastname: Drummen
mesh_terms: D000818:Animals; D017209:Apoptosis; D024141:Collagen Type IV / Q000235:genetics / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism / Q000473:pathology*; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism; D005355:Fibrosis; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D007668:Kidney / Q000378:metabolism / Q000473:pathology*; D007674:Kidney Diseases / Q000150:complications / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde; D018384:Oxidative Stress; D000091402:Prohibitins; D051381:Rats; D017208:Rats, Wistar; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D012720:Severity of Illness Index; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism; D014517:Ureteral Obstruction / Q000150:complications / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D024141:Collagen Type IV; D005353:Fibronectins; D000091402:Prohibitins; D012097:Repressor Proteins; D053773:Transforming Growth Factor beta1; D008315:Malondialdehyde