title: Comparative proteomic analysis on wild type and nitric oxide-overproducing mutant (nox1) of Arabidopsis thaliana.
abstract: Nitric oxide (NO) as a ubiquitous signal molecule plays an important role in plant development and growth. Here, we compared the proteomic changes between NO-overproducing mutant (nox1) and wild-type (WT) of Arabidopsis thaliana using two-dimensional electrophoresis coupled with MALDI-TOF MS. We successfully identified 59 differentially expressed proteins in nox1 mutant, which are predicted to play potential roles in specific cellular processes, such as post-translational modification, energy production and conversion, metabolism, transcription and signal transduction, cell rescue and defense, development and differentiation. Particularly, expression levels of five anti-oxidative enzymes were altered by the mutation; and assays of their respective enzymatic activities indicated an enhanced level of oxidative stress in nox1 mutant. Finally, some important proteins were further confirmed at transcriptional level using quantitative real-time PCR revealing the systemic changes between WT and nox1. The result suggests that obvious morphological changes in the nox1 mutant may be regulated by different mechanisms and factors, while excess endogenous NO maybe one of the possible reasons.
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361005, PR China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Hu
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361005, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Biology, Huaiyin Normal University, Huaian, Jiangsu 223300, PR China; Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361005, PR China.
authors.forename: Ting-Wu
authors.initials: TW
authors.lastname: Liu
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361005, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361005, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361005, PR China.
authors.forename: Fei-Hua
authors.initials: FH
authors.lastname: Wu
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361005, PR China.
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Hong Kong SAR, PR China.
authors.forename: Jun-Xian
authors.initials: JX
authors.lastname: He
authors.affiliation: Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei Institutes for Nationalities, Enshi 445000, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xiao
authors.affiliation: Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361005, PR China. Electronic address: zhenghl@xmu.edu.cn.
authors.forename: Hai-Lei
authors.initials: HL
authors.lastname: Zheng
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D017360:Arabidopsis / Q000378:metabolism*; D002734:Chlorophyll / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D009154:Mutation; D009569:Nitric Oxide / Q000378:metabolism*; D018384:Oxidative Stress; D010641:Phenotype; D010788:Photosynthesis; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction; D014158:Transcription, Genetic
chemical_list: D000975:Antioxidants; D010940:Plant Proteins; D002734:Chlorophyll; D009569:Nitric Oxide
keywords: Arabidopsis; Comparative proteomics; Development; Nitric oxide-overproducing mutant; nox1