title: Expression of matrix metalloproteinase-1 in round ligament and uterosacral ligament tissue from women with pelvic organ prolapse.
abstract: To evaluate the matrix metalloproteinase-1 (MMP-1) expression in different parts of pelvic connective tissue in postmenopausal women with and without pelvic organ prolapse (POP). Ninety-one samples were obtained from only postmenopausal women (42 with POP and 49 non-POP subjects). All women were evaluated by pelvic organ prolapse quantitation. The POP group had stage 2 or more, and the controls had stage 1 or less. Round ligament (RL) and uterosacral ligament (USL) biopsies were obtained from women with POP and controls. Immunohistochemistry for MMP-1 was performed on formalin-fixed and paraffin-embedded sections. The two groups were matched for age, body mass index, parity and postmenopausal status. MedCalc Statistical Software Programme Version 12.0.5 was used for statistical analysis. Expression of MMP-1 were significantly higher in both RL and USL tissue from postmenopausal women with POP, compared with controls. MMP-1 immunoreactivities were identified in both RL and USL biopsies from all women with and without POP. The expression pattern of MMP-1 were similar in these ligaments and were significantly higher in POP group compared with control subjects. These changes indicate a possible relation between MMP-1 expression of RL and USL in women with and without POP.
authors.affiliation: Department of Gynecology and Obstetrics, Goztepe Training and Research Hospital, Istanbul Medeniyet University, Istanbul, Turkey.
authors.forename: Akın
authors.initials: A
authors.lastname: Usta
authors.forename: Kadir
authors.initials: K
authors.lastname: Guzin
authors.forename: Mehmet
authors.initials: M
authors.lastname: Kanter
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ozgül
authors.forename: Ceyda Sancaklı
authors.initials: CS
authors.lastname: Usta
mesh_terms: D000368:Aged; D015992:Body Mass Index; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008022:Ligaments / Q000378:metabolism*; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D056887:Pelvic Organ Prolapse / Q000235:genetics / Q000378:metabolism*; D017698:Postmenopause; D017584:Reproductive History; D012404:Round Ligament of Uterus / Q000378:metabolism*
chemical_list: D020781:Matrix Metalloproteinase 1