title: Degradation of lipoxygenase-derived oxylipins by glyoxysomes from sunflower and cucumber cotyledons.
abstract: BACKGROUND
  Oilseed germination is characterized by the degradation of storage lipids. It may proceed either via the direct action of a triacylglycerol lipase, or in certain plant species via a specific lipid body 13-lipoxygenase. For the involvement of a lipoxygenase previous results suggested that the hydroxy- or oxo-group that is being introduced into the fatty acid backbone by this lipoxygenase forms a barrier to continuous β-oxidation.
  
  
  RESULTS
  This study shows however that a complete degradation of oxygenated fatty acids is possible by isolated cucumber and sunflower glyoxysomes. Interestingly, degradation is accompanied by the formation of saturated short chain acyl-CoAs with chain length between 4 and 12 carbon atoms lacking the hydroxy- or oxo-diene system of the oxygenated fatty acid substrate. The presence of these CoA esters suggests the involvement of a specific reduction of the diene system at a chain length of 12 carbon atoms including conversion of the hydroxy-group at C7.
  
  
  CONCLUSIONS
  To our knowledge this metabolic pathway has not been described for the degradation of polyunsaturated fatty acids so far. It may represent a new principle to degrade oxygenated fatty acid derivatives formed by lipoxygenases or chemical oxidation initiated by reactive oxygen species.
authors.forename: Danilo
authors.initials: D
authors.lastname: Meyer
authors.forename: Cornelia
authors.initials: C
authors.lastname: Herrfurth
authors.forename: Florian
authors.initials: F
authors.lastname: Brodhun
authors.affiliation: Georg-August-University, Albrecht-von-Haller Institute for Plant Sciences, Department for Plant Biochemistry, Justus-von-Liebig-Weg 11, Göttingen D-37075, Germany. ifeussn@uni-goettingen.de.
authors.forename: Ivo
authors.initials: I
authors.lastname: Feussner
mesh_terms: D018548:Cotyledon / Q000201:enzymology* / Q000378:metabolism; D018553:Cucumis sativus / Q000201:enzymology / Q000378:metabolism*; D064527:Etiolation; D020676:Glyoxysomes / Q000201:enzymology / Q000378:metabolism*; D006368:Helianthus / Q000201:enzymology / Q000378:metabolism*; D008041:Linoleic Acids / Q000378:metabolism; D008042:Linolenic Acids / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008084:Lipoxygenase / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D054883:Oxylipins / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism; D013997:Time Factors
chemical_list: D008041:Linoleic Acids; D008042:Linolenic Acids; D008054:Lipid Peroxides; D054883:Oxylipins; D009243:NAD; C038649:13-hydroperoxy-9,11-octadecadienoic acid; C064441:13-oxo-9,11-octadecadienoic acid; C117433:13-lipoxygenase; D008084:Lipoxygenase