title: Plasmid-dependent co-mutation in Streptomyces coelicolor A3(2).
abstract: A spontaneous chromosomalmutation(plc A(-)) in Streptomyces coelicolor A3(2) made the inborn plasmid SCP1 susceptible to curing by UV irradiation. Lack of the SCP1 plasmid (in SCP1(-) strains) prevented the occurrence of the co-mutation process after MNNG treatment, although it left the susceptibility to the lethal effect of the mutagen virtually unaffected. SCP1(-) strains were, however, ultrasensitive to the lethal effect of UV. Curing a plc A(-) strain of its SCPI plasmid made it refractory to co-mutation by MNNG and sensitive to the lethal effect of UV; reinfected by the plasmid, the strain resumed both the co-mutation proficiency and the UV-resistance. The occurrence on the SCP1 plasmid of a gene comparable to the uvrE gene of E. coli (Nevers and Spatz 1975) was assumed.
authors.affiliation: Istituto di Genetica, Università, via Arch irafi 22, 90123, Palermo, Italy.
authors.forename: A M
authors.initials: AM
authors.lastname: Puglia
authors.forename: F
authors.initials: F
authors.lastname: Misuracal
authors.forename: R
authors.initials: R
authors.lastname: Randazzo
authors.forename: G
authors.initials: G
authors.lastname: Sciandrellol
authors.forename: G
authors.initials: G
authors.lastname: Sermonti