title: The antioxidant and antigenotoxic potential of methanol extract of Cladonia foliacea (Huds.) Willd.
abstract: In this article, the genotoxic and antigenotoxic effects of methanol extract of of Cladonia foliacea (Huds.) Willd. (CME) were studied using WP2, Ames (TA1535 and TA1537), and sister chromatid exchange (SCE) test systems. The results of our studies showed that 5 µM concentration of aflatoxin B1(AFB1) changed the frequencies of SCE and malondialdehyde (MDA) levels, superoxide dismutase (SOD), glutathione (GSH), and glutathione peroxidase (GPx) activities. When 5 and 10 µg/mL concentrations of CME was added to AFB1, the frequencies of SCE and MDA level were decreased and SOD, GSH, and GPx levels were increased. The extract CME did not show any mutagenicity on Ames (Salmonella typhimurium TA1535, TA1537) and WP2 (Escherichia coli) test systems. On the other hand, CME has antimutagenicity on the mentioned test systems. The results of this experiment have clearly shown that CME has a significant antioxidative and antigenotoxic effect, which is thought to be due to the antigenotoxic activities of antioxidant enzymes.
authors.affiliation: Department of Biology Education, Kazim Karabekir Faculty of Education, Ataturk University, Erzurum, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Anar
authors.affiliation: Central Research and Application Laboratories, Agri Ibrahim Cecen University, Agri, Turkey Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey furkan_orhan@hotmail.com.
authors.forename: Furkan
authors.initials: F
authors.lastname: Orhan
authors.affiliation: Department of Biology, Faculty of Science, Fatih University, Istanbul, Turkey.
authors.forename: Lokman
authors.initials: L
authors.lastname: Alpsoy
authors.affiliation: Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey.
authors.forename: Medine
authors.initials: M
authors.lastname: Gulluce
authors.affiliation: Department of Biology Education, Kazim Karabekir Faculty of Education, Ataturk University, Erzurum, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Aslan
authors.affiliation: Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey.
authors.forename: Guleray
authors.initials: G
authors.lastname: Agar
mesh_terms: D000328:Adult; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008011:Lichens / Q000737:chemistry*; D000432:Methanol; D010088:Oxidoreductases; D012486:Salmonella typhimurium / Q000187:drug effects; D012854:Sister Chromatid Exchange / Q000187:drug effects; D055815:Young Adult
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D001688:Biological Products; D010088:Oxidoreductases; D000432:Methanol
keywords: Ames; Cladonia foliacea; SCE; WP2; antigenotoxicity; antioxidant enzymes