title: Novel protein PEGylation chemistry via glutalaldehyde-functionalized PEG.
abstract: Several PEGylated proteins have been approved as therapeutic drugs. In many cases, PEGylated protein has been synthesized by the conjugation reaction between PEG possessing activated ester and amine(s) in the protein. This reaction, however, often causes inactivation of PEGylated proteins. In this report, we present a novel chemistry which enables the PEGylation of proteins under the mild reaction condition. PEGylated lysozyme prepared by the method developed increased the biological activity of the PEGylated lysozyme more than 20 times compared with the PEGylated lysozyme prepared by the conventional method.
authors.affiliation: Department of Materials Science, Graduate School of Pure and Applied Sciences and ‡Master's School of Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba , Tennodai 1-1-1, Tsukuba, Ibaraki 305-8573, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Ikeda
authors.forename: Jinya
authors.initials: J
authors.lastname: Katamachi
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Kawasaki
authors.forename: Yukio
authors.initials: Y
authors.lastname: Nagasaki
mesh_terms: D000447:Aldehydes / Q000737:chemistry*; D015394:Molecular Structure; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D000447:Aldehydes; D011092:Polyethylene Glycols; D009113:Muramidase