title: Mammalian cell line developments in speed and efficiency.
abstract: Mammalian cell expression systems are the dominant tool today for producing complex biotherapeutic proteins. In this chapter, we discuss the basis for this dominance, and further explore why the Chinese hamster ovary (CHO) cell line has become the prevalent choice of hosts to produce most recombinant biologics. Furthermore, we explore some of the innovations that are currently in development to improve the CHO cell platform, from cell line specific technologies to overarching technologies that are designed to improve the overall workflow of bioprocess development.
authors.affiliation: Cell Culture Development, Biogen Idec , 15 Cambridge Center, Cambridge, MA, 02142, USA, Scott.Estes@biogenidec.com.
authors.forename: Scott
authors.initials: S
authors.lastname: Estes
authors.forename: Mark
authors.initials: M
authors.lastname: Melville
mesh_terms: D000818:Animals; D016466:CHO Cells / Q000145:classification / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D015195:Drug Design; D006801:Humans; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000627:therapeutic use*; D012371:Robotics / Q000379:methods*
chemical_list: D011994:Recombinant Proteins