title: Dieckol, a phlorotannin isolated from a brown seaweed, Ecklonia cava, inhibits adipogenesis through AMP-activated protein kinase (AMPK) activation in 3T3-L1 preadipocytes.
abstract: In this study, we assessed the potential inhibitory effect of 5 species of brown seaweeds on adipogenesis the differentiation of 3T3-L1 preadipocytes into mature adipocytes by measuring Oil-Red O staining. The Ecklonia cava extract tested herein evidenced profound adipogenesis inhibitory effect, compared to that exhibited by the other four brown seaweed extracts. Thus, E. cava was selected for isolation of active compounds and finally the three polyphenol compounds of phlorotannins were obtained and their inhibitory effect on adipogenesis was observed. Among the phlorotannins, dieckol exhibited greatest potential adipogenesis inhibition and down-regulated the expression of peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer-binding proteins (C/EBPα), sterol regulatory element-binding protein 1 (SREBP1) and fatty acid binding protein 4 (FABP4) in a dose-dependent manner. The specific mechanism mediating the effects of dieckol was confirmed by AMP-activated protein kinase (AMPK) activation. These results demonstrate inhibitory effect of dieckol compound on adipogenesis through the activation of the AMPK signal pathway.
authors.affiliation: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Seok-Chun
authors.initials: SC
authors.lastname: Ko
authors.forename: Myoungsook
authors.initials: M
authors.lastname: Lee
authors.forename: Ji-Hyeok
authors.initials: JH
authors.lastname: Lee
authors.forename: Seung-Hong
authors.initials: SH
authors.lastname: Lee
authors.forename: Yunsook
authors.initials: Y
authors.lastname: Lim
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Jeon
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D050156:Adipogenesis / Q000187:drug effects* / Q000235:genetics; D000818:Animals; D001391:Azo Compounds; D001572:Benzofurans / Q000633:toxicity*; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects; D004396:Coloring Agents; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D005612:Freeze Drying; D051379:Mice; D000459:Phaeophyceae / Q000737:chemistry*; D060888:Real-Time Polymerase Chain Reaction; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D001391:Azo Compounds; D001572:Benzofurans; D004396:Coloring Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; C503840:dieckol; D017868:Cyclic AMP-Dependent Protein Kinases; C022616:thiazolyl blue; C011049:oil red O
keywords: 3T3-L1 preadipocytes; AMPK; Adipogenesis; Diekol; Ecklonia cava