title: Characterization of a novel exopolysaccharide with antitumor activity from Lactobacillus plantarum 70810.
abstract: Three methods were used to prepare the cell-bound exopolysaccharides (c-EPS) of Lactobacillus plantarum 70810, and the maximum yield (64.17 mg/mL) was obtained by ultrasonic extraction. After anion exchange and gel filtration chromatography, the c-EPS was fractionated as a single peak with a molecular weight of 169.6 kD. Its structural characteristics were investigated by gas chromatography (GC), methylation, Fourier-transform infrared spectroscopy (FTIR), and nuclear magnetic resonance (NMR) analysis. Based on obtained data, the novel c-EPS was found to be a galactan containing a backbone of α-D-(1→6)-linked galactcosyl, β-D-(1→4)-linked galactcosyl, β-D-(1→2,3)-linked galactcosyl residues and a tail end of β-D-(1→)-linked galactcosyl residues. Preliminary in vitro tests revealed that c-EPS significantly inhibited the proliferation of HepG-2, BGC-823, especially HT-29 tumor cells. Our results suggested that the c-EPS produced by L. plantarum 70810 might be suitable for use as functional foods and natural antitumor drugs.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Rui
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Jiang
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: dongms@njau.edu.cn.
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Dong
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D056945:Hep G2 Cells; D006801:Humans; D048191:Lactiplantibacillus plantarum / Q000737:chemistry; D009369:Neoplasms / Q000188:drug therapy*; D011135:Polysaccharides, Bacterial / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D011135:Polysaccharides, Bacterial
keywords: Antitumor activity in vitro; Cell-bound exopolysaccharides (c-EPS); EPS; LAB; Lactobacillus plantarum; Mw; c-EPS; cell-bound exopolysaccharides; exopolysaccharides; lactic acid bacteria; molecular weight; r-EPS; release polysaccharides