title: Induction of apoptotic cell death in HL-60 cells by jacaranda seed oil derived fatty acids.
abstract: Various fatty acids are attracting considerable interest for their anticancer effects. Among them, fatty acids containing conjugated double bonds show one of the most potent cytotoxic effects on cancer cells. Here, we focused on the cancer cell killing activity of jacaranda seed oil. The seed oil of jacaranda harvested from Miyazaki in Japan contained 30.9% cis-8, trans-10, cis-12 octadecatrienoic acid, called jacaric acid (JA). Fatty acid prepared from this oil (JFA) and JA strongly induced cell death in human leukemia HL-60 cells. On the other hand, linoleic acid and trans-10, cis-12 conjugated linoleic acid (<10 μM) did not affect cell proliferation and viability. An increase in the sub-G₁ population and internucleosomal fragmentation of DNA was observed in JA- and JFA-treated cells, indicating induction of apoptotic cell death. Finally, the cytotoxic effects of JA and JFA were completely abolished by α-tocopherol. Taken together, these data suggest that jacaranda seed oil has potent apoptotic activity in HL-60 cells through induction of oxidative stress.
authors.affiliation: Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki.
authors.forename: Masao
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Chihiro
authors.initials: C
authors.lastname: Motonaga
authors.forename: Marino
authors.initials: M
authors.lastname: Yokoyama
authors.forename: Aya
authors.initials: A
authors.lastname: Ikezaki
authors.forename: Tomoka
authors.initials: T
authors.lastname: Kakihara
authors.forename: Rintaro
authors.initials: R
authors.lastname: Hayasegawa
authors.forename: Kaede
authors.initials: K
authors.lastname: Yamasaki
authors.forename: Masanobu
authors.initials: M
authors.lastname: Sakono
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Sakakibara
authors.forename: Masahito
authors.initials: M
authors.lastname: Suiko
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nishiyama
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D053938:DNA Fragmentation / Q000187:drug effects; D016193:G1 Phase / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D008042:Linolenic Acids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D009707:Nucleosomes / Q000235:genetics; D018384:Oxidative Stress / Q000187:drug effects; D010938:Plant Oils / Q000737:chemistry* / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry; D013329:Structure-Activity Relationship; D024502:alpha-Tocopherol / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D008042:Linolenic Acids; D009707:Nucleosomes; D010938:Plant Oils; C579118:jacaric acid; D024502:alpha-Tocopherol