title: Measurement of salivary cortisol by a chemiluminescent organic-based immunosensor.
abstract: A highly sensitive chemiluminescent immunoassay (CLIA) using a sensitive organic photodetector was developed to detect human cortisol, an important biomarker for stress-related diseases. The developed CLIA was performed onto gold-coated glass chips, on which anti-cortisol antibodies were immobilised and chemiluminescent horseradish peroxidase-luminol-peroxide reactions were generated. Using cortisol-spiked artificial saliva samples, the CLIA biosensor showed a linear range of detection between 0.1 ng/mL and 175 ng/mL and a detection limit of 80 pg/mL. The sensor response was highly specific to cortisol and did not vary significantly between assays. The results indicate the potential clinical application of the CLIA sensor. Furthermore, the simple layered structure of the organic photodetector may encourage the realisation of integrated optical biosensors for point-of-use measurement of salivary cortisol levels.
authors.affiliation: Department of Micro and Nano Systems Technology, Faculty of Technology and Maritime Sciences, Vestfold University College, Box 2243, N-3103 Tonsberg, Norway.
authors.forename: N M M
authors.initials: NM
authors.lastname: Pires
authors.forename: T
authors.initials: T
authors.lastname: Dong
mesh_terms: D015374:Biosensing Techniques; D002227:Carbazoles / Q000737:chemistry; D002234:Carbodiimides / Q000737:chemistry; D004123:Dimethylamines / Q000737:chemistry; D004563:Electrochemistry; D004581:Electronics; D004797:Enzyme-Linked Immunosorbent Assay; D004867:Equipment Design; D006046:Gold / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D006854:Hydrocortisone / Q000032:analysis / Q000378:metabolism*; D007118:Immunoassay; D049449:Luminescence; D008165:Luminol / Q000737:chemistry; D010545:Peroxides / Q000737:chemistry; D015203:Reproducibility of Results; D012464:Saliva, Artificial / Q000378:metabolism
chemical_list: D002227:Carbazoles; D002234:Carbodiimides; D004123:Dimethylamines; C569266:N-ethyl-N'-(3-(dimethylamino)-propyl)carbodiimide; D010545:Peroxides; D012464:Saliva, Artificial; D008165:Luminol; D006046:Gold; D006735:Horseradish Peroxidase; D006854:Hydrocortisone
keywords: Thin-film organic electronics; chemiluminescent immunoassay; optical biosensor; salivary cortisol