title: Chronic psychological stress suppresses contact hypersensitivity: potential roles of dysregulated cell trafficking and decreased IFN-γ production.
abstract: Increasing evidence shows that psychological stress can have dramatic impacts on the immune system, particularly the cutaneous immune response in dermatological disorders. While there have been many studies examining the impact of acute psychological stress on contact hypersensitivity there are relatively few studies concerning the impact of chronic psychological stress. Furthermore, the local immunological mechanisms by which chronic psychological stress impacts contact hypersensitivity still remain to be explored. Here we show that restraint-induced chronic psychological stress stimulates activation of the hypothalamus-pituitary-adrenal axis and delays weight gain in female BALB/c mice. We observed that chronic psychological stress reduces the cutaneous immune response as evidence by reduced ear swelling. This correlated with a significant decrease in the inflammatory cell infiltrate. On the other hand, chronic psychological stress does not influence T cell proliferation, activation, or sensitivity to corticosterone but does increase CD4(+) and CD8(+) T cell percentages in draining lymph nodes during a contact hypersensitivity reaction. Chronic psychological stress induces a decrease in overall circulating white blood cells, lymphocytes, and monocytes during a contact hypersensitivity reaction suggesting extravasation from the circulation. Finally, we found markedly reduced local IFN-γ production in chronically stressed animals. Based on these findings we propose that chronic psychological stress reduces contact hypersensitivity due to dysregulated cell trafficking and reduced production of IFN-γ.
authors.affiliation: Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, TX, United States.
authors.forename: Jessica M F
authors.initials: JM
authors.lastname: Hall
authors.affiliation: Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, TX, United States.
authors.forename: Alexandra R
authors.initials: AR
authors.lastname: Witter
authors.affiliation: Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, TX, United States.
authors.forename: Ronny R
authors.initials: RR
authors.lastname: Racine
authors.affiliation: Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, TX, United States.
authors.forename: Rance E
authors.initials: RE
authors.lastname: Berg
authors.affiliation: Department of Psychiatry and Behavioral Health, University of North Texas Health Science Center, Fort Worth, TX, United States.
authors.forename: Alan
authors.initials: A
authors.lastname: Podawiltz
authors.affiliation: Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, TX, United States.
authors.forename: Harlan
authors.initials: H
authors.lastname: Jones
authors.affiliation: Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, TX, United States; Department of Psychiatry and Behavioral Health, University of North Texas Health Science Center, Fort Worth, TX, United States; Department of Dermatology, University of Texas Southwestern Medical Center, Dallas, TX, United States. Electronic address: mark.mummert@unthsc.edu.
authors.forename: Mark E
authors.initials: ME
authors.lastname: Mummert
mesh_terms: D000818:Animals; D001835:Body Weight; D002465:Cell Movement; D002908:Chronic Disease; D003345:Corticosterone / Q000097:blood; D016207:Cytokines / Q000378:metabolism; D003877:Dermatitis, Contact / Q000276:immunology*; D004423:Ear / Q000473:pathology / Q000502:physiology; D005260:Female; D007030:Hypothalamo-Hypophyseal System / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis; D007962:Leukocytes / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010913:Pituitary-Adrenal System / Q000276:immunology*; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000276:immunology*; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D016207:Cytokines; D007371:Interferon-gamma; D003345:Corticosterone
keywords: Cell trafficking; Chronic psychological stress; Contact hypersensitivity