title: Full genome of influenza A (H7N9) virus derived by direct sequencing without culture.
abstract: An epidemic caused by influenza A (H7N9) virus was recently reported in China. Deep sequencing revealed the full genome of the virus obtained directly from a patient's sputum without virus culture. The full genome showed substantial sequence heterogeneity and large differences compared with that from embryonated chicken eggs.
authors.forename: Xianwen
authors.initials: X
authors.lastname: Ren
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Hu
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.forename: Liguo
authors.initials: L
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Dong
authors.forename: Lilian
authors.initials: L
authors.lastname: Sun
authors.forename: Yafang
authors.initials: Y
authors.lastname: Zhu
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiao
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: Jianwei
authors.initials: J
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Jin
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002681:China; D005260:Female; D016679:Genome, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D064766:Influenza A Virus, H7N9 Subtype / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D007251:Influenza, Human / Q000453:epidemiology / Q000821:virology; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013183:Sputum / Q000821:virology
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus
keywords: H7N9; avian-origin; culture-free; deep sequencing; direct sequencing; influenza; influenza A virus; viruses