title: Suspension and protoplast culture of U.S. rice cultivars.
abstract: Efficient protoplast culture and plant regeneration of five U.S. rice cultivars (Oryza sativa L.) - Mercury, Lacassine, Maybelle, Cypress, and Lemont - were obtained from suspension cells maintained in modified General Medium. Embryogenic suspension cells were developed from calli grown on the original callus induction medium for 10-20 weeks without subculture. Weekly subculture of the suspensions for five to eight weeks yielded cells suitable for protoplast isolation. After 2 weeks, rate of colony formation from protoplasts varied among the cultivars and ranged from 2.5 to 6.8%. Improvement of plating efficiencies to as high as 13.7% was obtained by conducting a second cycle of protoplast culture. A total of 525 plants were regenerated from the cultivars studied.
authors.affiliation: Rice Research Station, Louisiana State University Agricultural Center, P.O. Box 1429, 70527-1429, Crowley, LA, USA.
authors.forename: H S
authors.initials: HS
authors.lastname: Utomo
authors.forename: S S
authors.initials: SS
authors.lastname: Croughan
authors.forename: T P
authors.initials: TP
authors.lastname: Croughan