title: Chitosan-ionic liquid modified single-use sensor for electrochemical monitoring of sequence-selective DNA hybridization.
abstract: Chitosan-(CHIT) and ionic liquid- (1-butyl-3-methylimidazolium hexafluorophosphate (IL)) modified single-use graphite electrodes (PGEs) were developed for the first time in the present study for the enhanced monitoring of DNA, and also for sequence-selective DNA hybridization by measuring the guanine oxidation signal. The electrochemical behaviour of the CHIT-IL modified electrodes was first investigated (with unmodified electrodes as controls) using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). Sequence-selective DNA hybridization related to Hepatitis B virus (HBV) was also evaluated in the case of hybridization between amino-linked HBV probe and its complementary (target), a noncomplementary (NC) sequence, single base mismatch (MM), and also in the medium of target/mismatch (MM) mixtures (1:1). CHIT-IL modified PGEs presented a very effective discrimination of DNA hybridization owing to their superior selectivity and sensitivity.
authors.affiliation: Ege University, Faculty of Pharmacy, Analytical Chemistry Department, Bornova 35100, Izmir, Turkey. Electronic address: arzum.erdem@ege.edu.tr.
authors.forename: Arzum
authors.initials: A
authors.lastname: Erdem
authors.affiliation: Ege University, Faculty of Pharmacy, Analytical Chemistry Department, Bornova 35100, Izmir, Turkey; Adnan Menderes University, Faculty of Science, Chemistry Department, 09010 Aydın, Turkey.
authors.forename: Mihrican
authors.initials: M
authors.lastname: Muti
authors.affiliation: Ege University, Faculty of Pharmacy, Analytical Chemistry Department, Bornova 35100, Izmir, Turkey.
authors.forename: Fehmi
authors.initials: F
authors.lastname: Mese
authors.affiliation: Ege University, Faculty of Pharmacy, Analytical Chemistry Department, Bornova 35100, Izmir, Turkey.
authors.forename: Ece
authors.initials: E
authors.lastname: Eksin
mesh_terms: D015374:Biosensing Techniques; D002138:Calibration; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000378:metabolism*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry; D006147:Guanine / Q000737:chemistry; D007093:Imidazoles / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000235:genetics*; D010084:Oxidation-Reduction
chemical_list: C412621:1-butyl-3-methylimidazolium hexafluorophosphate; D007093:Imidazoles; D052578:Ionic Liquids; D006147:Guanine; D006108:Graphite; D004247:DNA; D048271:Chitosan
keywords: 1-Butyl-3-methylimidazolium hexafluorophosphate; Chitosan; DNA hybridization; Electrochemistry; Ionic liquid; Single-use graphite electrode