title: Long-term chlorhexidine effect on bond strength to Er:YAG laser irradiated-dentin.
abstract: This study evaluates the bond strength of dentin prepared with Er:YAG laser or bur, after rewetting with chlorhexidine on long-term artificial saliva storage and thermocycling. One hundred and twenty human third molars were sectioned in order to expose the dentin surface (n = 10). The specimens were randomly divided in 12 groups according to treatment and aging: Er:YAG laser rewetting with deionized water (LW) and 24 h storage in artificial saliva (WC); LW and 6 months of artificial saliva storage + 12.000 thermocycling (6M), LW and 12 months of artificial saliva storage + 24.000 thermocycling (12M), Er:YAG laser rewetting with 2% chlorhexidine (LC) and WC, LC and 6M, LC and 12M, bur on high-speed turbine rewetting with deionized water (TW) and WC, TW6M, TW12M, bur on high-speed turbine + 2% chlorhexidine (TC) and WC, TC and 6M, TC and 12M. The specimens were etched with 35% phosphoric acid, washed, and dried with air. Single Bond 2 adhesive was applied and the samples were restored with a composite. Each tooth was sectioned in order to obtain 4 sticks, which were submitted to microtensile bond strength test (µTBS). The two-way ANOVA, showed no significant differences for the interaction between the factors and for the aging factor. Tukey 5% showed that the LC group had the lowest µTBS. The rewetting with chlorhexidine negatively influenced the bond strength of the preparation with the Er:YAG laser. The artificial saliva aging and thermocycling did not interfere with dentin bond strength.
authors.affiliation: Department of Restorative Dentistry, Ribeirão Preto Dental School, University of São Paulo, Ribeirão Preto, São Paulo, 14040-904, Brazil.
authors.forename: Daniel
authors.initials: D
authors.lastname: Galafassi
authors.forename: Camila
authors.initials: C
authors.lastname: Scatena
authors.forename: Vivian
authors.initials: V
authors.lastname: Colucci
authors.forename: Antonio Luiz
authors.initials: AL
authors.lastname: Rodrigues-Júnior
authors.forename: Mônica
authors.initials: M
authors.lastname: Campos Serra
authors.forename: Silmara Aparecida Milori
authors.initials: SA
authors.lastname: Corona
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002710:Chlorhexidine / Q000494:pharmacology*; D001840:Dental Bonding; D003799:Dental Stress Analysis; D003804:Dentin / Q000737:chemistry / Q000187:drug effects* / Q000528:radiation effects*; D017220:Dentin-Bonding Agents / Q000494:pharmacology*; D006801:Humans; D053685:Laser Therapy; D008964:Molar, Third / Q000737:chemistry / Q000187:drug effects / Q000528:radiation effects; D013718:Tensile Strength; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D017220:Dentin-Bonding Agents; D002710:Chlorhexidine
keywords: bond strength; chlorhexidine; dentin; hybrid layer; laser; thermocycling