title: DHA but not EPA, enhances sound induced escape behavior and Mauthner cells activity in Sparus aurata.
abstract: Dietary omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), have a marked effect on fish behavior. There is limited information on the mechanisms involved in this effect and its relation to neuron development and functioning. Deficiency of n-3 LCPUFA reduces fish escape swimming. Mauthner cells (M-cell) are neurons responsible for initiating an escape response. The aim was to compare the effect of dietary DHA and EPA on escape behavior and neuronal activity of sea bream larvae. We studied burst swimming speed as a measure of behavior. M-cell activity was studied by ChAT immuno-fluorescence. Feeding the lowest n-3 LCPUFA levels a lower burst swimming speed. Increase in dietary EPA did not significantly improve escape response. Elevation of dietary DHA was correlated with a higher burst speed denoting the importance of this nutrient for escape swimming. Incorporation of DHA into larval tissues was proportional to DHA dietary levels and significantly correlated with burst speed. In addition, a higher immunoreactivity to ChAT, associated to a higher neural activity, was found in M-cell of larvae fed higher dietary DHA contents. These results show first evidence of n-3 LCPUFA on fish neuronal activity and their implications in behavior, denoting that DHA boosts escape swimming and this effect is at least partly mediated by the increase in neural activity of M-cell.
authors.affiliation: Grupo de Investigación en Acuicultura, Universidad de Las Palmas de Gran Canaria & Instituto Canario de Ciencias Marinas, P.O. Box 56, Telde, Las Palmas 35200, Canary Islands, Spain. Electronic address: tibi.benitez.santana@gmail.com.
authors.forename: Tibiábin
authors.initials: T
authors.lastname: Benítez-Santana
authors.forename: Eyad
authors.initials: E
authors.lastname: Atalah
authors.forename: Mónica Beatriz
authors.initials: MB
authors.lastname: Betancor
authors.forename: María José
authors.initials: MJ
authors.lastname: Caballero
authors.forename: Carmen Mari
authors.initials: CM
authors.lastname: Hernández-Cruz
authors.forename: Marisol
authors.initials: M
authors.lastname: Izquierdo
mesh_terms: D000161:Acoustic Stimulation; D000818:Animals; D002795:Choline O-Acetyltransferase; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D004924:Escape Reaction / Q000187:drug effects* / Q000502:physiology*; D007814:Larva; D009474:Neurons / Q000502:physiology*; D061848:Optical Imaging; D021541:Sea Bream / Q000502:physiology*; D013550:Swimming / Q000502:physiology
chemical_list: D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid; D002795:Choline O-Acetyltransferase
keywords: ARA; Burst swimming speed; DAH; DHA; EFA; EPA; Essential fatty acids; M-cell; Mauthner cells; Sea bream larvae; arachidonic acid; days after hatching; docosahexaenoic acid; eicosapentaenoic acid; essential fatty acids; n−3 LCPUFA; n−3 long chain polyunsaturated fatty acids