title: Gene expression during induction of somatic embryogenesis in carrot cell suspensions.
abstract: We report the identification, via their cDNAs, of genes which are temporarily transcribed during the initiation of somatic embryogenesis in carrot (Daucus carota L.) cells cultured in an auxin-free medium. Their expression is roughly associated with the first morphogenetic, or globular, stage. A cDNA library (λ gt 10) was established using poly(A)(+) -rich RNAs from cells deprived of auxin for 8 d. By differential screening a number of clones corresponding to early-induced embryogenic genes were identified. For several a temporary accumulation of the specific mRNA between 6 and 16 d after induction was observed. With regard to the nucleotide sequence and the respective deduced amino-acid sequence, two glycine-rich proteins and a polypeptide with a proline-rich domain were among the products of genes activated at the onset of somatic embryogenesis.
authors.affiliation: Institut für Botanik, Universität Hannover, Herrenhäuserstrasse 2, W-3000, Hannover 21, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Aleith
authors.forename: G
authors.initials: G
authors.lastname: Richter