title: Functionality of the pneumococcal antibody response in Down syndrome subjects.
abstract: We investigated the anti-polysaccharide antibody responses in subjects with Down syndrome (DS) because DS subjects show decreased peripheral B-lymphocyte numbers in all age groups, and a clinical picture of recurrent respiratory tract infections and increased incidence of autoimmune diseases which is reminiscent of common variable immunodeficiency disorders (CVID)-like disease. We determined titers and opsonophagocytosis in response to conjugated and unconjugated pneumococcal serotypes in 18 DS subjects aged 6-24 years. The results show adequate serotype-specific antibody titers in response to all conjugated and almost all unconjugated serotypes used. Opsonophagocytosis activity as measured against pneumococcal serotypes 9N, 19F and 23F was also found to be intact. We conclude that DS subjects do not have a clear defect in their anti-polysaccharide antibody response.
authors.affiliation: Department of Pediatrics, Jeroen Bosch Hospital, 's-Hertogenbosch, The Netherlands.
authors.forename: M A A
authors.initials: MA
authors.lastname: Kusters
authors.forename: N C C
authors.initials: NC
authors.lastname: Manders
authors.forename: B A W
authors.initials: BA
authors.lastname: de Jong
authors.forename: R W N M
authors.initials: RW
authors.lastname: van Hout
authors.forename: G T
authors.initials: GT
authors.lastname: Rijkers
authors.forename: E
authors.initials: E
authors.lastname: de Vries
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood*; D002648:Child; D004314:Down Syndrome / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009895:Opsonin Proteins / Q000097:blood*; D010587:Phagocytosis; D022242:Pneumococcal Vaccines / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D009895:Opsonin Proteins; D022242:Pneumococcal Vaccines; D011135:Polysaccharides, Bacterial
keywords: 23-valent pneumococcal polysaccharide vaccine; ADHS; Antibody response; CVID; DS; Down syndrome; FACS; FITC; IPD; Ig; MFI; OPA; Opsonophagocytosis; PCV-7; PI; PPV-23; Pneumococcal vaccines; RPMI; Roswell Park Memorial Institute medium; SPAD; T-cell dependent; T-cell independent; TD; TI; antibody diluted human serum; common variable immunodeficiency disorders; down syndrome; fluorescein isothiocyanate; fluorescence-activated cell sorting; heptavalent pneumococcal conjugate vaccine; immunoglobulin; invasive pneumococcal disease; mean fluorescence intensity; opsonophagocytosis assay; phagocytosis index; specific anti-polysaccharide antibody deficiency