title: A comparison of radiotracer and biochemical methods for the quantitation of experimental myocardial infarct weight: in vitro relationships.
abstract: Anterior myocardial infarction (MI) was produced in conscious dogs to evaluate the relationships among: a) cardiac technetium-99m stannous pyrophosphate (TcPPi) accretion, b) creatine phosphokinase (CPK) depletion, and c) postmortem MI weight, infarct structure, and histology. In vitro, there was a close relationship between measured MI weight and MI weight calculated by the TcPPi accretion (r = 0.96) or CPK depletion (r = 0.93) in representative "cross-sectional" MI samples. Cardiac TcPPi accretion and CPK depletion showed a curvilinear relationship over the spectrum of tissue samples. Adjacent to infarcts, there was marked TcPPi uptake and modest CPK depletion where histology suggested ischemia without infarction. Within infarcts, microscopically visible calcium was rare in this series, suggesting little intracellular calcium accumulation, insensitivity of the von Kossa staining technique, and/or other cellular mechanisms to account for Tc-PPi uptake in this conscious dog model without reperfusion.
authors.affiliation: University of Rochester School of Medicine, Rochester, New York, USA.
authors.forename: M W
authors.initials: MW
authors.lastname: Kronenberg
authors.forename: U R
authors.initials: UR
authors.lastname: Ettinger
authors.forename: G A
authors.initials: GA
authors.lastname: Wilson
authors.forename: E A
authors.initials: EA
authors.lastname: Schenk
authors.forename: J
authors.initials: J
authors.lastname: Cohen
mesh_terms: D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism; D004285:Dogs; D009203:Myocardial Infarction / Q000000981:diagnostic imaging* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D011849:Radioactive Tracers; D011877:Radionuclide Imaging; D016698:Technetium Tc 99m Pyrophosphate / Q000378:metabolism; D014003:Tin Polyphosphates / Q000378:metabolism
chemical_list: D011849:Radioactive Tracers; D014003:Tin Polyphosphates; C017742:technetium Tc 99m stannous pyrophosphate; D016698:Technetium Tc 99m Pyrophosphate; D003402:Creatine Kinase