title: Fimbria-fornix (FF)-transected hippocampal extracts induce the activation of astrocytes in vitro.
abstract: Hippocampus is one of the neurogenesis areas in adult mammals, but the function of astrocytes in this area is still less known. In our previous study, the fimbria-fornix (FF)-transected hippocampal extracts promoted the proliferation and neuronal differentiation of radial glial cells in vitro. To explore the effects of hippocampal extracts on gliogenesis, the hippocampal astrocytes were treated by normal or ff-transected hippocampal extracts in vitro. The cells were immunostained by brain lipid-binding protein (BLBP), nestin, and SOX2 to assess their state of activation. The effects of astrocyte-conditioned medium on the neuronal differentiation of hippocampal neural stem cells (NSCs) were also investigated. After treatment of FF-transected hippocampal extracts, the number of BLBP, nestin, and Sox-positive cells were obviously more than the cells which treated by normal hippocampal extracts, these cells maintained a state of activation and the activated astrocyte-conditioned medium also promoted the differentiation of NSCs into more neurons. These findings suggest that the astrocytes can be activated by FF-transected hippocampal extracts and these activated cells also can promote the neuronal differentiation of hippocampal NSCs in vitro.
authors.affiliation: Department of Human Anatomy and Histoembryology, Medical College of Soochow University, Suzhou, 215123, China.
authors.forename: Linqing
authors.initials: L
authors.lastname: Zou
authors.forename: Haoming
authors.initials: H
authors.lastname: Li
authors.forename: Guohua
authors.initials: G
authors.lastname: Jin
authors.forename: Meiling
authors.initials: M
authors.lastname: Tian
authors.forename: Jianbing
authors.initials: J
authors.lastname: Qin
authors.forename: Heyan
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D017077:Culture Media, Conditioned; D005260:Female; D005455:Fluorescent Antibody Technique; D020712:Fornix, Brain / Q000166:cytology*; D006624:Hippocampus / Q000166:cytology*; D008297:Male; D058953:Neural Stem Cells; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D017077:Culture Media, Conditioned