title: Pouring agar plates and streaking or spreading to isolate individual colonies.
abstract: Agar plates, composed of growth media solidified with agar, are commonly used to culture yeast and bacteria. Cultures may be spread or streaked across the surface of these plates to facilitate titer calculations or to permit isolation of single, genetically identical colonies.
authors.affiliation: Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: ehuang11@jhmi.edu.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Eyler
mesh_terms: D000362:Agar; D015169:Colony Count, Microbial / Q000379:methods*; D008828:Microbiological Techniques / Q000295:instrumentation / Q000379:methods*
chemical_list: D000362:Agar
keywords: Antibiotics/supplements; Isolate individual colonies; Pouring agar plates; Preparation of LB-agar solution; Spreading cells on agar plates; Streaking for individual colonies