title: Evolution of the hemagglutinin gene of the influenza A(H1N1)pdm09 virus in Morocco during two influenza seasons 2009-2011.
abstract: To study genetic evolution of Moroccan influenza A(H1N1)pdm09 virus strains, we conducted a molecular characterization of the hemagglutinin gene subunit 1 (HA1) of 36 influenza A(H1N1)pdm09 virus strains. The stains were collected from patients in Rabat and Casablanca during two influenza seasons 2009-2010 and 2010-2011. Nucleotide and amino acid sequences of 14 influenza A(H1N1)pdm09 virus strains from 2009 to 2010 were ~97 and 99 %, respectively, similar to the reference strain A/California/07/2009 (H1N1). Phylogenetic analysis of 22 influenza A(H1N1)pdm09 virus strains from 2010 to 2011 revealed a co-circulation of three well-described different genetic groups. Most important, none of the identified groups showed significant changes at the antigenic site of the virus HA1 subunit which may alter the efficacy of California/07/2009 (H1N1) vaccine.
authors.affiliation: Biosafety Level 3 and Research Laboratory (LRB-P3), Faculty of Medicine and Pharmacy, Mohammed V Military Teaching Hospital, University Mohammed V-Souissi, Hay Ryade, BP 190, Rabat, Morocco, elrhaffoulihicham@live.fr.
authors.forename: Hicham
authors.initials: H
authors.lastname: El Rhaffouli
authors.forename: El Mostafa
authors.initials: el M
authors.lastname: El Fahime
authors.forename: Abdellilah
authors.initials: A
authors.lastname: Laraqui
authors.forename: Tahar
authors.initials: T
authors.lastname: Bajjou
authors.forename: Marouane
authors.initials: M
authors.lastname: Melloul
authors.forename: Susan
authors.initials: S
authors.lastname: Obeid
authors.forename: Lahoussine
authors.initials: L
authors.lastname: Fathallah
authors.forename: Idriss
authors.initials: I
authors.lastname: Lahlou-Amine
mesh_terms: D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics; D016000:Cluster Analysis; D000939:Epitopes / Q000235:genetics; D019143:Evolution, Molecular; D005838:Genotype; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics* / Q000302:isolation & purification; D007251:Influenza, Human / Q000453:epidemiology / Q000821:virology*; D008969:Molecular Sequence Data; D009018:Morocco / Q000453:epidemiology; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000956:Antigens, Viral; D000939:Epitopes; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D012367:RNA, Viral