title: Comparative purification and characterization of two HIN domains, hematopoietic interferon-inducible nuclear antigens with a 200-amino-acid repeat, in murine AIM2-like receptors.
abstract: HIN (hematopoietic interferon-inducible nuclear antigens with a 200-amino-acid repeat) domains are found in all AIM2-like receptors (ALRs) and mediate protein/DNA interactions to regulate innate immunity. We cloned, expressed, and purified the human interferon-inducible protein p202 (Ifi202) HINb domain and the murine Ifi203 HIN domain using Escherichia coli JM109 (DE3) host cells. The Ifi203 HIN domain is a monomer in solution, and it has the ability to bind both double-stranded DNA and RNA. In contrast, the Ifi202 HINb domain is a dimer in solution, and no interaction with double-stranded DNA or RNA was detected. A complex of the Ifi203 HIN domain and double-stranded DNA was prepared, and crystals of the complex were obtained. To analyze further the dimeric interface of the Ifi202 HINb domain and the DNA binding site of the Ifi203 HIN domain, models of both proteins were developed. This work provides a basis for understanding the structure and function of HIN domains.
authors.affiliation: MOE Key Laboratory of Protein Sciences and Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University.
authors.forename: He
authors.initials: H
authors.lastname: Li
authors.forename: Zhi-Xin
authors.initials: ZX
authors.lastname: Wang
authors.forename: Jia-Wei
authors.initials: JW
authors.lastname: Wu
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007113:Immunity, Innate; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000276:immunology; D051379:Mice; D008958:Models, Molecular; D009687:Nuclear Proteins / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000276:immunology; D010802:Phylogeny; D011485:Protein Binding; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000276:immunology; D000071857:Tumor Suppressor p53-Binding Protein 1
chemical_list: C540422:Aim2 protein, mouse; D004268:DNA-Binding Proteins; C109045:Ifi203 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D011994:Recombinant Proteins; C498301:TP53BP1 protein, human; D000071857:Tumor Suppressor p53-Binding Protein 1; D012313:RNA; D004247:DNA