title: Linear ordered collagen scaffolds loaded with collagen-binding basic fibroblast growth factor facilitate recovery of sciatic nerve injury in rats.
abstract: Natural biological functional scaffolds, consisting of biological materials filled with promoting elements, provide a promising strategy for the regeneration of peripheral nerve defects. Collagen conduits have been used widely due to their excellent biological properties. Linear ordered collagen scaffold (LOCS) fibers are good lumen fillers that can guide nerve regeneration in an ordered direction. In addition, basic fibroblast growth factor (bFGF) is important in the recovery of nerve injury. However, the traditional method for delivering bFGF to the lesion site has no long-term effect because of its short half-life and rapid diffusion. Therefore, we fused a specific collagen-binding domain (CBD) peptide to the N-terminal of native basic fibroblast growth factor (NAT-bFGF) to retain bFGF on the collagen scaffolds. In this study, a natural biological functional scaffold was constructed using collagen tubes filled with collagen-binding bFGF (CBD-bFGF)-loaded LOCS to promote regeneration in a 5-mm rat sciatic nerve transection model. Functional evaluation, histological investigation, and morphometric analysis indicated that the natural biological functional scaffold retained more bFGF at the injury site, guided axon growth, and promoted nerve regeneration as well as functional restoration.
authors.affiliation: 1 The Affiliated Bayi Brain Hospital, The Military General Hospital of Beijing PLA , Beijing, China .
authors.forename: Fukai
authors.initials: F
authors.lastname: Ma
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Xiao
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.forename: Xianglin
authors.initials: X
authors.lastname: Hou
authors.forename: Jianwu
authors.initials: J
authors.lastname: Dai
authors.forename: Ruxiang
authors.initials: R
authors.lastname: Xu
mesh_terms: D000818:Animals; D002417:Cattle; D003094:Collagen / Q000737:chemistry* / Q000494:pharmacology*; D055724:Electrophysiological Phenomena / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000473:pathology; D009416:Nerve Regeneration / Q000187:drug effects*; D009929:Organ Size / Q000187:drug effects; D011485:Protein Binding / Q000187:drug effects; D017434:Protein Structure, Tertiary; D051381:Rats; D020127:Recovery of Function / Q000187:drug effects; D012584:Sciatic Nerve / Q000293:injuries* / Q000503:physiopathology* / Q000601:surgery / Q000637:transplantation; D013266:Stilbamidines / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D013266:Stilbamidines; D016222:Fibroblast Growth Factor 2; D003094:Collagen