title: Molecular cloning and expression of a C-type lectin gene from Venerupis philippinarum.
abstract: C-type lectins have been demonstrated to play important roles in invertebrate innate immunity by mediating the recognition of pathogens and clearing the micro-invaders. In the present study, a C-type lectin gene (denoted as VpCTL) was identified from Venerupis philippinarum by expressed sequence tag and rapid amplification of cDNA ends approaches. The full-length cDNA of VpCTL consists of 904 nucleotides with an open-reading frame of 456 bp encoding a peptide of 151 amino acids. The deduced amino acid sequence of VpCTL shared high similarity with C-type lectins from other species. The C-type lectin domain and the characteristic EPN and WND motifs were found in VpCTL. The VpCTL mRNA was dominantly expressed in the haemocytes of the V. philippinarum. After Listonella anguillarum challenge, the temporal expression of VpCTL mRNA in haemocytes was increased by 97- and 84-fold at 48 and 96 h, respectively. With high expression level in haemocytes and hepatopancreas, and the up-regulated expression in haemocytes indicted that VpCTL was perhaps involved in the immune responses to L. anguillarum challenge.
authors.affiliation: School of Marine Science of Ningbo University, Ningbo, 315211, People's Republic of China.
authors.forename: Changkao
authors.initials: C
authors.lastname: Mu
authors.forename: Leilei
authors.initials: L
authors.lastname: Chen
authors.forename: Jianmin
authors.initials: J
authors.lastname: Zhao
authors.forename: Chunlin
authors.initials: C
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D003001:Cloning, Molecular; D015870:Gene Expression; D005786:Gene Expression Regulation / Q000276:immunology; D006434:Hemocytes / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D054884:Host-Pathogen Interactions; D037181:Lectins, C-Type / Q000235:genetics* / Q000378:metabolism; D053526:Listonella / Q000502:physiology; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny
chemical_list: D037181:Lectins, C-Type