title: Increase in spleen volume of rats on experimental model of pre-hepatic portal hypertension.
abstract: BACKGROUND
  Pressure increase in the portal venous system, caused by reduction of blood supply to the liver, develops collateral circulation of splanchnic vessel that reaches the venous system of superior vena cava. The spleen works as an auxiliary capacitance system which compensates the increase in portal pressure that may cause splenomegaly.
  
  
  OBJECTIVE
  To reproduce experimental model of pre-hepatic portal hypertension by reducing the caliber of the vein, allowing the study of alterations in the rat's spleen in this situation.
  
  
  METHODS
  Fifteen Wistar rats were divided into three groups of five. All animals were operated, being the first five used as control (group 1) and reoperated after 15 days (sham-operated), along with five other animals had portal hypertension induced (group 2). The last five animals, also with portal hypertension were reoperated after 30 days (group 3). Prehepatic portal hypertension was caused in experimental animals by partial ligation of portal vein in hepatic hilum. The spleen was measured during the first and second operations, allowing the calculation of its volume in the three groups studied using specific mathematical formula.
  
  
  RESULTS
  No macroscopic changes in spleen were significant when compared the animals reoperated after 15 days (group 2) to the ones after 30 days (group 3), being 15 days considered enough for the evaluation of effects on spleen after partial ligation. Although the volume of spleens studied in groups 2 and 3 have increased after the first operation - ranging on average from 0.5417 cm(3) to 0.6345 cm(3) at reoperation with increase of approximately 17% in volume between the first and second phase - the rsults were not statistically significant (p> 0.05).
  
  
  CONCLUSIONS
  There was no statistically significant increase in the spleen volume after partial ligation of the portal vein when comparing the calculated volume in the first operation to the volume found at reoperation. There was no difference when comparing the spleen volumes of the animals reoperated after 15 days and the ones reoperated after 30 days.
authors.forename: Aline Riquena da
authors.initials: AR
authors.lastname: Silva
authors.forename: Ricardo José
authors.initials: RJ
authors.lastname: Kriguer-Júnior
authors.forename: Leonardo Carvalho
authors.initials: LC
authors.lastname: Serigiolle
authors.forename: Helbert Minuncio Pereira
authors.initials: HM
authors.lastname: Gomes
authors.forename: Daren Athiê Boy
authors.initials: DA
authors.lastname: Rodrigues
authors.forename: Pedro Luiz Squilacci
authors.initials: PL
authors.lastname: Leme
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006975:Hypertension, Portal; D008026:Ligation; D008099:Liver; D011169:Portal Vein / Q000601:surgery; D051381:Rats; D017208:Rats, Wistar; D013163:Splenomegaly