title: Thidiazuron-induced high frequency of shoot induction and plant regeneration in protoplast derived pea callus.
abstract: Protoplasts isolated from lateral shoot buds of cotyledon-free pea embryo axes were regenerated to callus. Protoplast derived calluses with a diameter of about 1cm were transferred to shoot induction media, containing different concentrations (1-50µM) of thidiazuron. Shoot formation was observed after 16 weeks up to 12% efficiency. Thidiazuron (10µM) was the most effective concentration in all experiments. Shoot buds elongated in medium supplemented with N-isopentenyl adenine and indole-3-butyric acid. Since rooting was almost impossible in these thidiazuron-induced shoots, shoots were grafted onto young pea seedlings and regenerated to fertile plants.
authors.affiliation: Lehrgebiet Molekulargenetik, Universität Hannover, Herrenhäuserstr. 2, D-30419, Hannover, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Böhmer
authors.forename: B
authors.initials: B
authors.lastname: Meyer
authors.forename: H J
authors.initials: HJ
authors.lastname: Jacobsen