title: Identification of anti-adipogenic proteins in adult bovine serum suppressing 3T3-L1 preadipocyte differentiation.
abstract: Adipocyte differentiation is a complex developmental process forming adipocytes from various precursor cells. The murine 3T3-L1 preadipocyte cell line has been most frequently used in the studies of adipocyte differentiation. Differentiation of 3T3-L1 preadipocytes includes a medium containing fetal bovine serum (FBS) with hormonal induction. In this study, we observed that differentiation medium containing adult bovine serum (ABS) instead of FBS did not support differentiation of preadipocytes. Impaired adipocyte differentiation was due to the presence of a serum protein factor in ABS that suppresses differentiation of preadipocytes. Using a proteomic analysis, alpha-2-macroglobulin and paraoxonase/arylesterase 1, which were previously shown to suppress differentiation of preadipocytes, were identified as anti-adipogenic proteins. Although their functional mechanisms have not yet been elucidated, the anti-adipogenic effects of these proteins are discussed.
authors.forename: Jeongho
authors.initials: J
authors.lastname: Park
authors.forename: Jihyun
authors.initials: J
authors.lastname: Park
authors.forename: Sang-Soep
authors.initials: SS
authors.lastname: Nahm
authors.forename: Inho
authors.initials: I
authors.lastname: Choi
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Korea kimjihoe@ynu.ac.kr.
authors.forename: Jihoe
authors.initials: J
authors.lastname: Kim
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D043303:Aryldialkylphosphatase / Q000032:analysis* / Q000378:metabolism; D001798:Blood Proteins / Q000494:pharmacology*; D002417:Cattle; D003470:Culture Media / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D057075:Enzyme Assays; D051379:Mice; D040901:Proteomics; D000511:alpha-Macroglobulins / Q000032:analysis* / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D003470:Culture Media; D000511:alpha-Macroglobulins; D043303:Aryldialkylphosphatase