title: Co-expression of RCH10 and AGLU1 confers rice resistance to fungal sheath blight Rhizoctonia solani and blast Magnorpathe oryzae and reveals impact on seed germination.
abstract: Rice sheath blight and blast caused by Rhizoctonia solani Kühn and Magnorpathe oryzae respectively, are the two most destructive fungal diseases in rice. With no genetic natural traits conferring resistance to sheath blight, transgenic manipulation provides an obvious approach. In this study, the rice basic chitinase gene (RCH10) and the alfalfa β-1,3-glucanase gene (AGLU1) were tandemly inserted into transformation vector pBI101 under the control of 35S promoter with its enhancer sequence to generate a double-defense gene expression cassette pZ100. The pZ100 cassette was transformed into rice (cv. Taipei 309) by Agrobacterium-mediated transformation. More than 160 independent transformants were obtained and confirmed by PCR. Northern analysis of inheritable progenies revealed similar levels of both RCH10 and AGLU1 transcripts in the same individuals. Disease resistance to both sheath blight and blast was challenged in open field inoculation. Immunogold detection revealed that RCH10 and AGLU1 proteins were initially located mainly in the chloroplasts and were delivered to the vacuole and cell wall upon infection, suggesting that these subcellular compartments act as the gathering and execution site for these anti-fungal proteins. We also observed that transgenic seeds display lower germination rate and seedling vigor, indicating that defense enhancement might be achieved at the expense of development.
authors.affiliation: State Key Laboratory of Rice Biology and Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China, maobz@zju.edu.cn.
authors.forename: Bizeng
authors.initials: B
authors.lastname: Mao
authors.forename: Xuehui
authors.initials: X
authors.lastname: Liu
authors.forename: Dongwei
authors.initials: D
authors.lastname: Hu
authors.forename: Debao
authors.initials: D
authors.lastname: Li
mesh_terms: D002688:Chitinases / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D043326:Glucan 1,3-beta-Glucosidase / Q000235:genetics / Q000378:metabolism*; D020082:Magnaporthe / Q000276:immunology*; D016254:Mutagenesis, Insertional; D012275:Oryza / Q000276:immunology* / Q000382:microbiology; D063245:Plant Development; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000276:immunology* / Q000382:microbiology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012232:Rhizoctonia / Q000276:immunology*; D014170:Transformation, Genetic
chemical_list: D011994:Recombinant Proteins; D002688:Chitinases; D043326:Glucan 1,3-beta-Glucosidase