title: Characterization of thermosensitive mutants of Physarum polycephalum a plasmodial screening method for cell cycle mutants based upon synchronization at the restrictive temperature.
abstract: A screening procedure for cell cycle mutants among is mutants of Physarum has been developed for unsynchronized microplasmodia. The synchronization of the microplasmodia and the ratio of pre- to post-mitotic nuclei were evaluated after a transient shift-up to the non-permissive temperature. In contrast with wild type and is mutants not blocked in a cell cycle event, putative cell cycle mutants were synchronized. Among them, three strains which showed a reduction of DNA synthesis had an abnormally increased ratio of pre- to post-mitotic nuclei. Thus, this methodology seems to be efficient and more rapid than the previously published procedures for detecting cell-cycle mutants of Physarum.
authors.affiliation: Laboratoire de Pharmacologie et de Toxicologie Fondamentales, CNRS, 205 route de Narbonne, F-31078, Toulouse Cedex, France.
authors.forename: M
authors.initials: M
authors.lastname: Wright
authors.forename: L
authors.initials: L
authors.lastname: Del Castillo
authors.forename: M L
authors.initials: ML
authors.lastname: Oustrin