title: Gene expression profile regulated by the HPV16 E7 oncoprotein and estradiol in cervical tissue.
abstract: The HPV16 E7 oncoprotein and 17β-estradiol are important factors for the induction of premalignant lesions and cervical cancer. The study of these factors is crucial for a better understanding of cervical tumorigenesis. Here, we assessed the global gene expression profiles induced by the HPV16 E7 oncoprotein and/or 17β-estradiol in cervical tissue of FvB and K14E7 transgenic mice. We found that the most dramatic changes in gene expression occurred in K14E7 and FvB groups treated with 17β-estradiol. A large number of differentially expressed genes involved in the immune response were observed in 17β-estradiol treated groups. The E7 oncoprotein mainly affected the expression of genes involved in cellular metabolism. Our microarray data also identified differentially expressed genes that have not previously been reported in cervical cancer. The identification of genes regulated by E7 and 17β-estradiol, provides the basis for further studies on their role in cervical carcinogenesis.
authors.affiliation: Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados (Cinvestav), Mexico City 07360, Mexico; Research Unit, Hospital Juárez de México, Mexico City 07760, Mexico. Electronic address: enoc.cortes@salud.gob.mx.
authors.forename: Enoc M
authors.initials: EM
authors.lastname: Cortés-Malagón
authors.forename: José
authors.initials: J
authors.lastname: Bonilla-Delgado
authors.forename: José
authors.initials: J
authors.lastname: Díaz-Chávez
authors.forename: Alfredo
authors.initials: A
authors.lastname: Hidalgo-Miranda
authors.forename: Sandra
authors.initials: S
authors.lastname: Romero-Cordoba
authors.forename: Aykut
authors.initials: A
authors.lastname: Uren
authors.forename: Haydar
authors.initials: H
authors.lastname: Celik
authors.forename: Matthew
authors.initials: M
authors.lastname: McCormick
authors.forename: José A
authors.initials: JA
authors.lastname: Munguía-Moreno
authors.forename: Eloisa
authors.initials: E
authors.lastname: Ibarra-Sierra
authors.forename: Jaime
authors.initials: J
authors.lastname: Escobar-Herrera
authors.forename: Paul F
authors.initials: PF
authors.lastname: Lambert
authors.forename: Daniel
authors.initials: D
authors.lastname: Mendoza-Villanueva
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Bermudez-Cruz
authors.forename: Patricio
authors.initials: P
authors.lastname: Gariglio
mesh_terms: D000818:Animals; D004958:Estradiol / Q000494:pharmacology*; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D054884:Host-Pathogen Interactions; D051379:Mice; D008822:Mice, Transgenic; D050725:Papillomavirus E7 Proteins / Q000378:metabolism*
chemical_list: D004967:Estrogens; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16; D004958:Estradiol
keywords: 17β-estradiol; Carcinogenesis; Cervix; HPV16 E7; K14E7; Microarrays