title: New method of screening allergenic components from Shuanghuanglian injection: with RBL-2H3/CMC model online HPLC/MS system.
abstract: Shuanghuanglian (SHL) injection is a traditional Chinese medicine (TCM) injection widely used in China to treat influenza, tonsillitis, bronchitis, and faucitis. Adverse drug reactions (ADR) of SHL injection, majorly manifested as allergic reactions, were among the leading causes of death from TCM injection. In this study, an RBL-2H3/CMC online LC/MS system was established to screen and identify allergenic components in SHL injection, by which Baicalin was identified as a potential allergenic component. Allergenic activities of baicalin were investigated in RBL-2H3 cell degranulation and β-hexosaminidase release tests in vitro. Our results showed that baicalin dose-dependently induced RBL-2H3 cell degranulation and β-hexosaminidase release in the range of 0-50μg/ml. The RBL-2H3/CMC online LC/MS system developed in this study may potentially be used to screen allergenic components in other TCM injections.
authors.affiliation: School of Medicine, Xi'an Jiaotong University, 76#, Yantan Westroad, Xi'an 710061, China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Han
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.forename: Ronghua
authors.initials: R
authors.lastname: Cui
authors.forename: Langchong
authors.initials: L
authors.lastname: He
mesh_terms: D000485:Allergens / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000009:adverse effects* / Q000032:analysis / Q000737:chemistry*; D006967:Hypersensitivity; D013058:Mass Spectrometry; D009862:Online Systems; D010936:Plant Extracts / Q000737:chemistry; D051381:Rats; D013678:Technology, Pharmaceutical; D001619:beta-N-Acetylhexosaminidases / Q000737:chemistry
chemical_list: D000485:Allergens; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C516400:shuang-huang-lian; D001619:beta-N-Acetylhexosaminidases
keywords: Anaphylactoid reaction; Cell degranulation; Liquid chromatography tandem mass spectrum; RBL-2H3 cell membrane chromatography; β-Hexosaminidase release