title: WT1 isoform expression pattern in acute myeloid leukemia.
abstract: WT1 plays a dual role in leukemia development, probably due to an imbalance in the expression of the 4 main WT1 isoforms. We quantify their expression and evaluate them in a series of AML patients. Our data showed a predominant expression of isoform D in AML, although in a lower quantity than in normal CD34+ cells. We found a positive correlation between the total WT1 expression and A, B and C isoforms. The overexpression of WT1 in AML might be due to a relative increase in A, B and C isoforms, together with a relative decrease in isoform D expression.
authors.affiliation: Department of Hematology, Hospital Universitari i Politècnic La Fe, Valencia, Spain. Electronic address: luna_ire@gva.es.
authors.forename: Irene
authors.initials: I
authors.lastname: Luna
authors.forename: Esperanza
authors.initials: E
authors.lastname: Such
authors.forename: Jose
authors.initials: J
authors.lastname: Cervera
authors.forename: Eva
authors.initials: E
authors.lastname: Barragán
authors.forename: Mariam
authors.initials: M
authors.lastname: Ibañez
authors.forename: Inés
authors.initials: I
authors.lastname: Gómez-Seguí
authors.forename: María
authors.initials: M
authors.lastname: López-Pavía
authors.forename: Marta
authors.initials: M
authors.lastname: Llop
authors.forename: Oscar
authors.initials: O
authors.lastname: Fuster
authors.forename: Sandra
authors.initials: S
authors.lastname: Dolz
authors.forename: Silvestre
authors.initials: S
authors.lastname: Oltra
authors.forename: Carmen
authors.initials: C
authors.lastname: Alonso
authors.forename: Belén
authors.initials: B
authors.lastname: Vera
authors.forename: Ignacio
authors.initials: I
authors.lastname: Lorenzo
authors.forename: David
authors.initials: D
authors.lastname: Martínez-Cuadrón
authors.forename: Pau
authors.initials: P
authors.lastname: Montesinos
authors.forename: M Leonor
authors.initials: ML
authors.lastname: Senent
authors.forename: Federico
authors.initials: F
authors.lastname: Moscardó
authors.forename: Pascual
authors.initials: P
authors.lastname: Bolufer
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Sanz
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D015331:Cohort Studies; D005260:Female; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020033:Protein Isoforms / Q000235:genetics; D014407:Tumor Cells, Cultured; D025721:WT1 Proteins / Q000235:genetics*; D055815:Young Adult
chemical_list: D020033:Protein Isoforms; D025721:WT1 Proteins; C583751:WT1 protein, human
keywords: Acute myeloid leukemia; WT1 isoforms