title: Effect of huanglianjiedu tang on fever in rats induced by 2, 4-dinitrophenol.
abstract: OBJECTIVE
  To study metabolic characteristics of fever in rats induced by 2, 4-dinitrophenol (DNP) and the effect of Huanglianjiedu Tang (HLJDT) on the fever.
  
  
  METHODS
  The urine samples were analyzed by ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) at the positive ion mode scanning, and experimental data were analyzed by the principal component analysis.
  
  
  RESULTS
  Eight potential biomarkers indicating the occurrence and evolvement of fever were determined according to ions in urine samples. Five of them were found increased, while the other three decreased. After HLJDD intervention, the increased five were reduced significantly in high dose group, compared with model group, while the decreased three had no obvious change. Five of the eight biomakers were identified with formyl-5-hydroxykynurenamine, gentisic acid, aminoadipic acid, phenylacetic acid, L-phenylalanyl-L-hydroxyproline on the basis of MS/MS. These biomarkers are associated with the metabolism of 5-hydroxytryptamine, tyrosine, lysine, phenylalanine and collagen protein, respectively.
  
  
  CONCLUSIONS
  HLJDT had significant effect on DNP-induced fever in rats. The effect was performed possibly by acting on 5-hydroxytryptamine in hypothalamus and some amino acid metabolism. These results suggested that HLJDT relieved fever by acting on multi-targets.
authors.affiliation: Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Liu
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.forename: Pingping
authors.initials: P
authors.lastname: Chen
authors.forename: Xuzhao
authors.initials: X
authors.lastname: Li
authors.forename: Changfeng
authors.initials: C
authors.lastname: Liu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D015415:Biomarkers / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D004140:Dinitrophenols / Q000009:adverse effects*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005334:Fever / Q000188:drug therapy* / Q000209:etiology / Q000652:urine; D006801:Humans; D008297:Male; D055432:Metabolomics; D051381:Rats; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D004140:Dinitrophenols; D004365:Drugs, Chinese Herbal