title: Dynamic modeling and validation of a lignocellulosic enzymatic hydrolysis process--a demonstration scale study.
abstract: The enzymatic hydrolysis process is one of the key steps in second generation biofuel production. After being thermally pretreated, the lignocellulosic material is liquefied by enzymes prior to fermentation. The scope of this paper is to evaluate a dynamic model of the hydrolysis process on a demonstration scale reactor. The following novel features are included: the application of the Convection-Diffusion-Reaction equation to a hydrolysis reactor to assess transport and mixing effects; the extension of a competitive kinetic model with enzymatic pH dependency and hemicellulose hydrolysis; a comprehensive pH model; and viscosity estimations during the course of reaction. The model is evaluated against real data extracted from a demonstration scale biorefinery throughout several days of operation. All measurements are within predictions uncertainty and, therefore, the model constitutes a valuable tool to support process optimization, performance monitoring, diagnosis and process control at full-scale studies.
authors.affiliation: Department of Electrical Engineering, Automation and Control Group, Technical University of Denmark, Elektrovej Building 326, 2800 Kgs. Lyngby, Denmark. Electronic address: rmpr@elektro.dtu.dk.
authors.forename: Remus Mihail
authors.initials: RM
authors.lastname: Prunescu
authors.forename: Gürkan
authors.initials: G
authors.lastname: Sin
mesh_terms: D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002138:Calibration; D002475:Cellobiose / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D003198:Computer Simulation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D008962:Models, Theoretical; D015203:Reproducibility of Results; D014783:Viscosity; D014990:Xylans / Q000378:metabolism; D014994:Xylose / Q000378:metabolism
chemical_list: D014990:Xylans; C036909:lignocellulose; D002475:Cellobiose; D002482:Cellulose; D008031:Lignin; D014994:Xylose; D002480:Cellulase; D005947:Glucose
keywords: Biofuel; Demonstration scale; Dynamic modeling; Enzymatic hydrolysis; Sensitivity and uncertainty analysis