title: Neuroprotective effects of tanshinone I from Danshen extract in a mouse model of hypoxia-ischemia.
abstract: Hypoxia-ischemia leads to serious neuronal damage in some brain regions and is a strong risk factor for stroke. The aim of this study was to investigate the neuroprotective effect of tanshinone I (TsI) derived from Danshen (Radix Salvia miltiorrhiza root extract) against neuronal damage using a mouse model of cerebral hypoxia-ischemia. Brain infarction and neuronal damage were examined using 2,3,5-triphenyltetrazolium chloride (TTC) staining, hematoxylin and eosin histochemistry, and Fluoro-Jade B histofluorescence. Pre-treatment with TsI (10 mg/kg) was associated with a significant reduction in infarct volume 1 day after hypoxia-ischemia was induced. In addition, TsI protected against hypoxia-ischemia-induced neuronal death in the ipsilateral region. Our present findings suggest that TsI has strong potential for neuroprotection against hypoxic-ischemic damage. These results may be used in research into new anti-stroke medications.
authors.affiliation: Department of Neurobiology, Kangwon National University School of Medicine, Chuncheon, Korea.
authors.forename: Jae-Chul
authors.initials: JC
authors.lastname: Lee
authors.forename: Joon Ha
authors.initials: JH
authors.lastname: Park
authors.forename: Ok Kyu
authors.initials: OK
authors.lastname: Park
authors.forename: In Hye
authors.initials: IH
authors.lastname: Kim
authors.forename: Bing Chun
authors.initials: BC
authors.lastname: Yan
authors.forename: Ji Hyeon
authors.initials: JH
authors.lastname: Ahn
authors.forename: Seung-Hae
authors.initials: SH
authors.lastname: Kwon
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Choi
authors.forename: Jong-Dai
authors.initials: JD
authors.lastname: Kim
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
keywords: Hypoxia-ischemia; Neuronal death; Neuroprotection; Radix Salvia miltiorrhiza