title: A simple method for isolation, liquid culture, transformation and regeneration of Arabidopsis thaliana protoplasts.
abstract: An efficient technique was developed for the isolation, culture, transformation and regeneration of protoplasts derived from auxin conditioned Arabidopsis root cultures. On an average 30% of root protoplasts underwent cell division in liquid culture and formed somatic embryolike structures which regenerated to plants without embedding in Ca(2+)-alginate. The protoplast protocol was applicable to different landraces of Arabidopsis thaliana (L.) Heynh., such as RLD, Columbia or C24. PEG-mediated DNA uptake into protoplasts using different uidA reporter gene constructs yielded transient gene expression in over 25% of treated cells indicating that root-derived protoplasts are suitable recipients for transformation.
authors.affiliation: Institute of Plant Biology, Biological Research Center of Hungarian Academy of Sciences, H-6726 Szeged, P.O. Box 521, 62, Temesvári krt., Hungary.
authors.forename: J
authors.initials: J
authors.lastname: Mathur
authors.forename: C
authors.initials: C
authors.lastname: Koncz
authors.forename: L
authors.initials: L
authors.lastname: Szabados