title: [MiR-24 improves beta-like globin gene expression through targeting Sp1].
abstract: We studied the function and mechanism of miR-24 in regulating beta-like globin gene expression. We first detected the expression of miR-24 during erythroid differentiation and also detected the globin gene expression in miR-24 overexpressing K562 cells through q-PCR. Dual-luciferase reporter assay and Western blotting were used to identify target genes of miR-24. "Rescue experiment" was further used to investigate the regulation of miR-24 on globin gene expression whether depending on targeting Sp1 or not. We found that miR-24 increased during hemin-induced K562 cells and EPO-induced HPCs (hematopoietic progenitor cells) erythroid differentiation. Overexpression of miR-24 in K562 cells promoted the epsilon- and gamma-globin gene expression during hemin-induced erythroid differentiation through targeting the negative globin regulator Sp1. These results suggested that miR-24 can improve the expression of beta-like globin gene through targeting Sp1.
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College (PUMC), Beijing 100005, China.
authors.forename: Yanni
authors.initials: Y
authors.lastname: Ma
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Bei
authors.initials: B
authors.lastname: Gong
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Hualu
authors.initials: H
authors.lastname: Zhao
authors.forename: Junwu
authors.initials: J
authors.lastname: Zhang
authors.forename: Jia
authors.initials: J
authors.lastname: Yu
mesh_terms: D002454:Cell Differentiation; D005786:Gene Expression Regulation; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D020014:K562 Cells; D035683:MicroRNAs / Q000235:genetics*; D016329:Sp1 Transcription Factor / Q000235:genetics*; D055547:epsilon-Globins / Q000235:genetics*; D055545:gamma-Globins / Q000235:genetics*
chemical_list: C543845:MIRN24 microRNA, human; D035683:MicroRNAs; D016329:Sp1 Transcription Factor; C108209:SP1 protein, human; D055547:epsilon-Globins; D055545:gamma-Globins