title: Accelerated alcoholic fermentation caused by defective gene expression related to glucose derepression in Saccharomyces cerevisiae.
abstract: Sake yeast strains maintain high fermentation rates, even after the stationary growth phase begins. To determine the molecular mechanisms underlying this advantageous brewing property, we compared the gene expression profiles of sake and laboratory yeast strains of Saccharomyces cerevisiae during the stationary growth phase. DNA microarray analysis revealed that the sake yeast strain examined had defects in expression of the genes related to glucose derepression mediated by transcription factors Adr1p and Cat8p. Furthermore, deletion of the ADR1 and CAT8 genes slightly but statistically significantly improved the fermentation rate of a laboratory yeast strain. We also identified two loss-of-function mutations in the ADR1 gene of existing sake yeast strains. Taken together, these results indicate that the gene expression program associated with glucose derepression for yeast acts as an impediment to effective alcoholic fermentation under glucose-rich fermentative conditions.
authors.affiliation: National Research Institute of Brewing.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Watanabe
authors.forename: Naoya
authors.initials: N
authors.lastname: Hashimoto
authors.forename: Megumi
authors.initials: M
authors.lastname: Mizuno
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Takeshi
authors.initials: T
authors.lastname: Akao
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shimoi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000235:genetics*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010750:Phosphoproteins; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D015534:Trans-Activators / Q000172:deficiency / Q000235:genetics*; D014157:Transcription Factors / Q000172:deficiency / Q000235:genetics*
chemical_list: C074682:ADR1 protein, S cerevisiae; C092600:CAT8 protein, S cerevisiae; D004268:DNA-Binding Proteins; D010750:Phosphoproteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D000431:Ethanol; D005947:Glucose