title: Development-specific differences in the proteomics of Angiostrongylus cantonensis.
abstract: Angiostrongyliasis is an emerging communicable disease. Several different hosts are required to complete the life cycle of Angiostrongylus cantonensis. However, we lack a complete understanding of variability of proteins across different developmental stages and their contribution to parasite survival and progression. In this study, we extracted soluble proteins from various stages of the A. cantonensis life cycle [female adults, male adults, the fifth-stage female larvae (FL5), the fifth-stage male larvae (ML5) and third-stage larvae (L3)], separated those proteins using two-dimensional difference gel electrophoresis (2D-DIGE) at pH 4-7, and analyzed the gel images using DeCyder 7.0 software. This proteomic analysis produced a total of 183 different dominant protein spots. Thirty-seven protein spots were found to have high confidence scores (>95%) by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Comparative proteomic analyses revealed that 29 spots represented cytoskeleton-associated proteins and functional proteins. Eight spots were unnamed proteins. Twelve protein spots that were matched to the EST of different-stage larvae of A. cantonensis were identified. Two genes and the internal control 18s were chosen for quantitative real-time PCR (qPCR) and the qPCR results were consistent with those of the DIGE studies. These findings will provide a new basis for understanding the characteristics of growth and development of A. cantonensis and the host-parasite relationship. They may also assist searches for candidate proteins suitable for use in diagnostic assays and as drug targets for the control of eosinophilic meningitis caused by A. cantonensis.
authors.affiliation: Department of Parasitology, Medical College of Soochow University, Suzhou, Jiangsu, P. R. China ; Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China.
authors.forename: Hui-Cong
authors.initials: HC
authors.lastname: Huang
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Yao
authors.forename: Zeng-Mei
authors.initials: ZM
authors.lastname: Song
authors.forename: Xing-Pan
authors.initials: XP
authors.lastname: Li
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Hua
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Chang-Wang
authors.initials: CW
authors.lastname: Pan
authors.forename: Chao-Ming
authors.initials: CM
authors.lastname: Xia
mesh_terms: D017185:Angiostrongylus cantonensis / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017238:Genes, Helminth / Q000235:genetics; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism*; D006790:Host-Parasite Interactions; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008018:Life Cycle Stages; D008297:Male; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012908:Snails / Q000469:parasitology; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017206:Strongylida Infections / Q000469:parasitology
chemical_list: D015801:Helminth Proteins; D020543:Proteome