title: Whole genome analysis of epizootic hemorrhagic disease virus identified limited genome constellations and preferential reassortment.
abstract: Epizootic hemorrhagic disease virus (EHDV) is a Culicoides transmitted orbivirus that causes haemorrhagic disease in wild and domestic ruminants. A collection of 44 EHDV isolated from 2008 to 2012 was fully sequenced and analysed phylogenetically. Serotype 2 viruses were the dominant serotype all years except 2012 when serotype 6 viruses represented 63 % of the isolates. High genetic similarity (>94 % identity) between serotype 1 and 2 virus VP1, VP3, VP4, VP6, NS1, NS2 and NS3 segments prevented identification of reassortment events for these segments. Additionally, there was little genetic diversity (>96 % identity) within serotypes for VP2, VP5 and VP7. Preferential reassortment within the homologous serotype was observed for VP2, VP5 and VP7 segments for type 1 and type 2 viruses. In contrast, type 6 viruses were all reassortants containing VP2 and VP5 derived from an exotic type 6 with the remaining segments most similar to type 2 viruses. These results suggest that reassortment between type 1 and type 2 viruses requires conservation of the VP2, VP5 and VP7 segment constellation while type 6 viruses only require VP2 and VP5 and are restricted to type 2-lineage VP7. As type 6 VP2 and VP5 segments were exclusively identified in viruses with type 2-derived VP7, these results suggest functional complementation between type 2 and type 6 VP7 proteins.
authors.affiliation: Newport Laboratories, 1520 Prairie Drive, Worthington, Minnesota 56187, USA.
authors.forename: Srivishnupriya
authors.initials: S
authors.lastname: Anbalagan
authors.affiliation: Newport Laboratories, 1520 Prairie Drive, Worthington, Minnesota 56187, USA.
authors.forename: Elyse
authors.initials: E
authors.lastname: Cooper
authors.affiliation: Newport Laboratories, 1520 Prairie Drive, Worthington, Minnesota 56187, USA.
authors.forename: Pat
authors.initials: P
authors.lastname: Klumper
authors.affiliation: Newport Laboratories, 1520 Prairie Drive, Worthington, Minnesota 56187, USA.
authors.forename: Randy R
authors.initials: RR
authors.lastname: Simonson
authors.affiliation: Newport Laboratories, 1520 Prairie Drive, Worthington, Minnesota 56187, USA.
authors.forename: Ben M
authors.initials: BM
authors.lastname: Hause
mesh_terms: D016000:Cluster Analysis; D014644:Genetic Variation; D016679:Genome, Viral; D005838:Genotype; D018164:Hemorrhagic Disease Virus, Epizootic / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D016865:Reassortant Viruses / Q000235:genetics; D011995:Recombination, Genetic; D012088:Reoviridae Infections / Q000821:virology; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D012367:RNA, Viral