title: Structure elucidation and antioxidant properties of a soluble β-D-glucan from mushroom Entoloma lividoalbum.
abstract: A water soluble branched β-D-glucan (PS-I) with an average molecular weight ~2.1×10(5) Da was isolated from alkaline extract of the fruit bodies of the edible mushroom Entoloma lividoalbum (Kühner & Romagn) Kubička which consists of terminal β-D-glucopyranosyl, (1→3)-β-D-glucopyranosyl, (1→6)-β-D-glucopyranosyl, and (1→3,6)-β-D-glucopyranosyl moieties in a molar ratio of nearly 1:3:2:1. The structure of PS-I was elucidated using acid hydrolysis, methylation analysis, periodate oxidation study, partial hydrolysis, and 1D/2D NMR experiments. The repeating unit of the polysaccharide (PS-I) contains a backbone chain of three (1→6)-β-D-glucopyranosyl residues, one of which was branched at O-3 position with the side chain consisting of three (1→3)-β-D-glucopyranosyl and a terminal β-D-glucopyranosyl residues. Total antioxidant capacity of 1mg PS-I was measured and found equivalent to 70±15 μg of ascorbic acid. The PS-I was found to possess hydroxyl and superoxide radical-scavenging activities with EC50 values of 480 and 150 μg/mL, respectively. The reducing power of PS-I was determined 0.5 at 480 μg/mL.
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Prasenjit
authors.initials: P
authors.lastname: Maity
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Surajit
authors.initials: S
authors.lastname: Samanta
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Ashis K
authors.initials: AK
authors.lastname: Nandi
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Ipsita K
authors.initials: IK
authors.lastname: Sen
authors.affiliation: Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, West Bengal, India.
authors.forename: Soumitra
authors.initials: S
authors.lastname: Paloi
authors.affiliation: Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, West Bengal, India.
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Acharya
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India. Electronic address: sirajul_1999@yahoo.com.
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D001205:Ascorbic Acid / Q000737:chemistry / Q000378:metabolism; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D005936:Glucans / Q000737:chemistry* / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D012995:Solubility; D013481:Superoxides / Q000737:chemistry; D014867:Water
chemical_list: D000975:Antioxidants; D005936:Glucans; D011134:Polysaccharides; D014867:Water; D013481:Superoxides; D001205:Ascorbic Acid
keywords: Antioxidant properties; Entoloma lividoalbum (Kühner & Romagn) Kubička; β-d-Glucan