title: Transgenic plants of ramie (Boehmeria nivea Gaud.) obtained by Agrobacterium mediated transformation.
abstract: A regeneration and transformation protocol for ramie (Boehmeria nivea Gaud.) is presented. Regeneration was obtained from leaf discs placed on solid B-5 medium (Gamborg et al. 1968) containing adequate concentrations of auxin and cytokinin. Co-cultivation of leaf discs with Agrobacterium tumefaciens and subsequent regeneration resulted in transgenic plants as shown by Southern blot and analysis of expression of the GUS-marker gene.
authors.affiliation: EMBRAPA/CENARGEN, SAIN Parque Rural, C.P. 02372, 70849-970, Brasília DF, Brasil.
authors.forename: D M
authors.initials: DM
authors.lastname: Dusi
authors.forename: M
authors.initials: M
authors.lastname: Dubald
authors.forename: E R
authors.initials: ER
authors.lastname: de Almeida
authors.forename: L S
authors.initials: LS
authors.lastname: Caldas
authors.forename: E S
authors.initials: ES
authors.lastname: Gander