title: Differentiated epithelial- and mesenchymal-like phenotypes in subcutaneous mouse xenografts using diffusion weighted-magnetic resonance imaging.
abstract: Epithelial-mesenchymal transition (EMT) is important for tumor metastasis. Detection of EMT protein expression and observation of morphological changes are commonly used to identify EMT. Diffusion-weighted magnetic resonance imaging (DW-MRI) and measuring apparent diffusion coefficient (ADC) values are noninvasive techniques for characterizing tumor microenvironments. We investigated the difference in ADC values between epithelial- and mesenchymal-like subcutaneous mouse xenografted tumors using DW-MRI. Epithelial-like MM189 PB-Klf4 and BL322 PB-Klf4 cells were generated from tumor suppressive Kruppel-like factor 4 (Klf4)-expressing mesenchymal-like MM189 and BL322 cells. The ADC values of xenografted tumors from epithelial-like MM189 PB-Klf4 and BL322 PB-Klf4 were significantly lower than those from their mesenchymal-like counterparts (p < 0.05 and p < 0.01, respectively). Our results suggested that DW-MRI is a potential tool for observing mesenchymal- or epithelial-like characteristics of subcutaneous xenografted tumors.
authors.affiliation: National Institute of Cancer Research, National Health Research Institutes, Miaoli 350, Taiwan. r2207759@ms19.hinet.net.
authors.forename: Ya-Wen
authors.initials: YW
authors.lastname: Chen
authors.forename: Huay-Ben
authors.initials: HB
authors.lastname: Pan
authors.forename: Hui-Hwa
authors.initials: HH
authors.lastname: Tseng
authors.forename: Hsiao-Chien
authors.initials: HC
authors.lastname: Chu
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Hung
authors.forename: Yi-Chen
authors.initials: YC
authors.lastname: Yen
authors.forename: Chen-Pin
authors.initials: CP
authors.lastname: Chou
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D038524:Diffusion Magnetic Resonance Imaging; D058750:Epithelial-Mesenchymal Transition; D006801:Humans; D000090062:Kruppel-Like Factor 4; D008648:Mesoderm / Q000378:metabolism / Q000473:pathology; D051379:Mice; D009362:Neoplasm Metastasis / Q000175:diagnosis* / Q000503:physiopathology; D009369:Neoplasms / Q000175:diagnosis* / Q000473:pathology; D059016:Tumor Microenvironment; D023041:Xenograft Model Antitumor Assays