title: Genetic transformation ofPelargonium X hortorum.
abstract: TransgenicPelargonium X hortorum have been producedvia Agrobacterium tumefaciens-mediated transformation. The regeneration protocol used provided a regeneration frequency approximately to 95 percent. Clumps of regenerants, from cotyledons and hypocotyls ofPelargonium X hortorum seedlings, were inoculated with the disarmed strain EHA101 ofAgrobacterium tumefaciens. This strain contains a binary vector carrying neomycin phosphotransferase II, hygromycin B phosphotransferase and ß-glucuronidase genes. Selection on the regeneration medium supplemented with hygromycin allowed production of transgenic plants in up to 20% of the inoculated explants. The insertion of foreign DNA was demonstrated by Southern and polymerase chain reaction analysis: these experiments indicated that the inserted T-DNA is not full length for most of the plants. All RO transgenic plants exhibited a normal phenotype and are fertile.
authors.affiliation: INRA, C.R. d'Angers, Station d'Amélioration des Espèces Fruitières et Ornementales, F-49071, Beaucouzé, France.
authors.forename: M P
authors.initials: MP
authors.lastname: Robichon
authors.forename: J P
authors.initials: JP
authors.lastname: Renou
authors.forename: R
authors.initials: R
authors.lastname: Jalouzot