title: Plant regeneration from callus and protoplast cultures of Helianthus giganteus L.
abstract: Methods of plant regeneration from callus and protoplasts of Helianthus giganteus L. are described. Embryogenic callus was obtained from leaf explants and plants were regenerated from these calli on MS media with different combinations of benzyladenine and naphtaleneacetic acid. Leaf protoplasts isolated from in vitro grown plants formed somatic embryos when cultured in agarose solidified droplets of V-KM medium containing benzyladenine and naphtaleneacetic acid. Embryos developed into plantlets on media with reduced auxin contents. Regenerated plants were successfully planted in soil.
authors.affiliation: Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, POB 521, H-6701, Szeged, Hungary.
authors.forename: S
authors.initials: S
authors.lastname: Krasnyanski
authors.forename: Z
authors.initials: Z
authors.lastname: Polgár
authors.forename: G
authors.initials: G
authors.lastname: Németh
authors.forename: L
authors.initials: L
authors.lastname: Menczel