title: Efficient plant regeneration from Indica (group 1) rice protoplasts of one advanced breeding line and three varieties.
abstract: Regenerable embryogenic suspensions were established from one Indica (group 1) rice advanced breeding line and 9 Indica (group 1) rice varieties in 6-8 weeks. Four were chosen for protoplast culture and plant regeneration. About 4-7×10(7) protoplasts were isolated from one gram of 8-week-old cell suspension. High plating efficiency (30.5%) and colony formation (13.7%) were obtained using nurse culture methods. A high plant regeneration frequency (67.5%) was observed for line IR57311-95-2-3. In total, 322 plants were regenerated. All the regenerated plants were fertile.
authors.affiliation: Tissue Culture Laboratory, The International Rice Research Institute, P.O. Box 933, 1099, Manila, Philippines.
authors.forename: S
authors.initials: S
authors.lastname: Zhang