title: Utilization of human DC-SIGN and L-SIGN for entry and infection of host cells by the New World arenavirus, Junín virus.
abstract: The target cell tropism of enveloped viruses is regulated by interactions between viral proteins and cellular receptors determining susceptibility at a host cell, tissue or species level. However, a number of additional cell-surface moieties can also bind viral envelope glycoproteins and could act as capture receptors, serving as attachment factors to concentrate virus particles on the cell surface, or to disseminate the virus infection to target organs or susceptible cells within the host. Here, we used Junín virus (JUNV) or JUNV glycoprotein complex (GPC)-pseudotyped particles to study their ability to be internalized by the human C-type lectins hDC- or hL-SIGN. Our results provide evidence that hDC- and hL-SIGN can mediate the entry of Junín virus into cells, and may play an important role in virus infection and dissemination in the host.
authors.affiliation: Department of Microbiology & Immunology, College of Veterinary Medicine, Cornell University, Ithaca NY 14853, USA.|Laboratorio de Virología, Departamento de Química Biológica IQUIBICEN - CONICET. Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (UBA), Ciudad Universitaria, Pabellón II, Piso 4, 1428, Buenos Aires, Argentina.
authors.forename: M Guadalupe
authors.initials: MG
authors.lastname: Martinez
authors.affiliation: Department of Microbiology & Immunology, College of Veterinary Medicine, Cornell University, Ithaca NY 14853, USA.
authors.forename: Michele A
authors.initials: MA
authors.lastname: Bialecki
authors.affiliation: Department of Microbiology & Immunology, College of Veterinary Medicine, Cornell University, Ithaca NY 14853, USA.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Belouzard
authors.affiliation: Laboratorio de Virología, Departamento de Química Biológica IQUIBICEN - CONICET. Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (UBA), Ciudad Universitaria, Pabellón II, Piso 4, 1428, Buenos Aires, Argentina.
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Cordo
authors.affiliation: Laboratorio de Virología, Departamento de Química Biológica IQUIBICEN - CONICET. Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (UBA), Ciudad Universitaria, Pabellón II, Piso 4, 1428, Buenos Aires, Argentina.
authors.forename: Nélida A
authors.initials: NA
authors.lastname: Candurra
authors.affiliation: Department of Microbiology & Immunology, College of Veterinary Medicine, Cornell University, Ithaca NY 14853, USA.
authors.forename: Gary R
authors.initials: GR
authors.lastname: Whittaker
mesh_terms: D016475:3T3 Cells; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002522:Chlorocebus aethiops; D006478:Hemorrhagic Fever, American / Q000378:metabolism* / Q000821:virology*; D054884:Host-Pathogen Interactions; D006801:Humans; D018051:Junin virus / Q000502:physiology*; D037181:Lectins, C-Type / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011990:Receptors, Transferrin / Q000378:metabolism; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000378:metabolism; D053586:Virus Internalization; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin; D014759:Viral Envelope Proteins; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C068126:CD71 antigen; C433029:CLEC4M protein, human; C555941:GPC protein, junin virus
keywords: Junín arenavirus; Lectin; Virus entry; Virus receptor