title: Micro-solid phase extraction with liquid chromatography-tandem mass spectrometry for the determination of aflatoxins in coffee and malt beverage.
abstract: A single step extraction-cleanup procedure using porous membrane-protected micro-solid phase extraction (μ-SPE) in conjunction with liquid chromatography-tandem mass spectrometry for the extraction and determination of aflatoxins (AFs) B1, B2, G1 and G2 from food was successfully developed. After the extraction, AFs were desorbed from the μ-SPE device by ultrasonication using acetonitrile. The optimum extraction conditions were: sorbent material, C8; sorbent mass, 20mg; extraction time, 90 min; stirring speed, 1,000 rpm; sample volume, 10 mL; desorption solvent, acetonitrile; solvent volume, 350 μL and ultrasonication period, 25 min without salt addition. Under the optimum conditions, enrichment factor of 11, 9, 9 and 10 for AFG2, AFG1, AFB2 and AFB1, respectively were achieved. Good linearity and correlation coefficient was obtained over the concentration range of 0.4-50 ng g(-1) (r(2) 0.9988-0.9999). Good recoveries for AFs ranging from 86.0-109% were obtained. The method was applied to 40 samples involving malt beverage (19) and canned coffee (21). No AFs were detected in the selected samples.
authors.affiliation: School of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.
authors.forename: Wejdan Shakir
authors.initials: WS
authors.lastname: Khayoon
authors.forename: Bahruddin
authors.initials: B
authors.lastname: Saad
authors.forename: Baharuddin
authors.initials: B
authors.lastname: Salleh
authors.forename: Normaliza Hj Abdul
authors.initials: NH
authors.lastname: Manaf
authors.forename: Aishah A
authors.initials: AA
authors.lastname: Latiff
mesh_terms: D000348:Aflatoxins / Q000737:chemistry* / Q000302:isolation & purification*; D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003069:Coffee / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D052617:Solid Phase Microextraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000348:Aflatoxins; D003069:Coffee
keywords: Aflatoxins; Food; Liquid chromatography–tandem mass spectrometry; Micro-solid phase extraction; Mycotoxins