title: Agrobacterium mediated genetic transformation and plant regeneration via organogenesis and somatic embryogenesis from cotyledon leaves in eggplant (Solanum melongena L. cv. 'Kecskeméti lila').
abstract: Novel and efficient protocols for plant regeneration and genetic transformation from longitudinally-halved cotyledons ofin vitro raised seedlings in eggplant (Solanum melongena L.) are described. After co-cultivation withAgrobacterium vectors harboring neomycin phosphotransferase (nptll) as selectable marker, transgenic plantlets were regenerated on selective media containing 100 mg/l kanamycin. Transformants were recovered from embryogenic calli induced by 4 mg/lα-naphthaleneacetic acid (NAA), and from organogenic calli induced by the addition of 2 mg/l zeatin plus 0.01 mg/l NAA. Nineteen independent transgenic lines were grown to maturity. The structural integrity, expression and sexual transmission of the introduced genes for neomycin phosphotransferase and ß-glucuronidase (gus) were investigated.
authors.affiliation: Agricultural Biotechnology Center, Institute for Plant Sciences, Laboratory for Cell Biology and Tissue Culture, Szent-Györgyi A. u. 4, H-2100, Gödöllö, Hungary.
authors.forename: M
authors.initials: M
authors.lastname: Fári
authors.forename: I
authors.initials: I
authors.lastname: Nagy
authors.forename: M
authors.initials: M
authors.lastname: Csányi
authors.forename: J
authors.initials: J
authors.lastname: Mitykó
authors.forename: A
authors.initials: A
authors.lastname: Andrásfalvy