title: Studies of interaction between terbium(III)-deferasirox and double helix DNA by spectral and electrochemical methods.
abstract: DNA binding studies of terbium(III)-deferasirox (Tb3+-DFX) complex were monitored to understand the reaction mechanism and introduce a new probe for the assay of DNA. In the present work, UV absorption spectrophotometry, fluorescence spectroscopy, circular dichroism (CD), cyclic voltammetry (CV) and viscosity measurement were employed to study the interactions of Tb3+-DFX with calf thymus DNA (ctDNA). The binding of Tb3+-DFX complex to ctDNA showed a hyperchromic effect in the absorption spectra and the increase in fluorescence quenching effect (amount) of Tb3+-DFX complex in the presence of ctDNA. The binding constants (Kb) for the complex with ctDNA were estimated to be 1.8×10(4) M(-1) through UV absorption spectrophotometry and fluorescence spectroscopy. Upon addition of the complex, clear decreases were observed in the viscosity of ctDNA. The CD spectra indicated that there are certain detectable conformational changes in the DNA double helix when the complex was added. The CV method showed that both anodic and cathodic peak potentials of Tb3+-DFX complex showed negative shifts on the addition of the ctDNA. Further, competitive methylene blue binding studies with fluorescence spectroscopy have shown that the complex can bind to ctDNA through nonintercalative mode. The experimental results suggest that Tb3+-DFX complex binds to DNA via groove binding and/or electrostatic binding mode.
authors.affiliation: Department of Chemistry, University of Payame Noor, P.O. Box. 19395-3697, Tehran, Iran.
authors.forename: Masoomeh
authors.initials: M
authors.lastname: Shaghaghi
authors.affiliation: Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran. Electronic address: dehghan2001d@yahoo.com.
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Dehghan
authors.affiliation: Drug Applied Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Abolghasem
authors.initials: A
authors.lastname: Jouyban
authors.affiliation: Department of Biochemistry, University of Payame Noor, P.O. Box. 19395-3697, Tehran, Iran.
authors.forename: Parisa
authors.initials: P
authors.lastname: Sistani
authors.affiliation: Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran.
authors.forename: Mitra
authors.initials: M
authors.lastname: Arvin
mesh_terms: D000818:Animals; D001565:Benzoates / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D000077588:Deferasirox; D055664:Electrochemical Techniques; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013725:Terbium / Q000737:chemistry / Q000378:metabolism*; D014230:Triazoles / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001565:Benzoates; D005456:Fluorescent Dyes; D007502:Iron Chelating Agents; D014230:Triazoles; D013725:Terbium; D004247:DNA; C427203:calf thymus DNA; D000077588:Deferasirox
keywords: Binding mode; DNA interaction; Fluorescent probe; Spectroscopic approach; Terbium complex