title: PCR detection of transcripts homologous to the self-incompatibility gene in anthers ofBrassica.
abstract: The polymerase chain reaction (PCR) is particularly well suited for the detection of rare sequences. Taking advantage of the recent isolation of sequences associated with stigma self-incompatibility inBrassica oleracea, we used PCR amplifications with primers synthesized to the S6 cDNA sequence, to demonstrate the presence of mRNA homologous to stigmaS-locus gene (SLG) in anthers during early microsporogenesis. In addition, otherS-locus-related (SLR) sequences were shown to be transcribed in sexual as well as in vegetative tissues (roots, leaves), suggesting that the SLG family might be involved not only in pollen-stigma recognition, but more generally in various forms of plant cell signalling processes. This information corroborates the recent discovery of a cDNA-deduced protein kinase from maize roots, whose extracellular receptor displays high homology withBrassica S-locus-specific glycoproteins.
authors.affiliation: , .
authors.forename: C M
authors.initials: CM
authors.lastname: Guilluy
authors.forename: M
authors.initials: M
authors.lastname: Trick
authors.forename: P
authors.initials: P
authors.lastname: Heizmann
authors.forename: C
authors.initials: C
authors.lastname: Dumas