title: RFLP tagging of a new semidwarfing gene in rice.
abstract: A new rice semidwarfing gene which is not allelic tosd1, temporarily designated assdg, might be of use as a new source of semidwarfism in rice breeding programs. We report here the identification of a DNA marker closely linked to this gene. The DNA marker was identified by testing 120 mapped rice RFLP makers as hybridization probes for Southern analysis of a pair of nearly isogenic lines with or withoutsdg. Linkage association of the marker with the gene was verified using a F2 population segregating for semidwarfism. RFLP analysis showed thatsdg is closely linked to a single-copy DNA clone RZ182 on chromosome 5, with a distance of 4.3 centiMorgans between them. This marker may facilitate early selection for the semidwarfing gene in rice breeding programs.
authors.affiliation: Institute of Genetics, Academia Sinica, 100101, Beijing, PR China.
authors.forename: C Z
authors.initials: CZ
authors.lastname: Liang
authors.forename: M H
authors.initials: MH
authors.lastname: Gu
authors.forename: X B
authors.initials: XB
authors.lastname: Pan
authors.forename: G H
authors.initials: GH
authors.lastname: Liang
authors.forename: L H
authors.initials: LH
authors.lastname: Zhu