title: Transient gene expression in strawberry (Fragaria x ananassa Duch.) protoplasts and the recovery of transgenic plants.
abstract: A transient ß-glucuronidase (GUS)-assay was performed to evaluate electroporation parameters and optimize DNA delivery conditions into strawberry protoplasts. Optimal GUS-activity was obtained when protoplasts were subjected to 400 V/cm for 20 ms. GUS-activity could be further increased by the addition of carrier DNA to the electroporation mixture. Callus selected on 10 μg/ml hygromycin produced shoots which exhibited GUS-activity. The transformed nature of the shoots obtained after selection was confirmed by DNA-analysis.
authors.affiliation: Department of Physiological Botany, University of Uppsala, Box 540, S-751 21, Uppsala, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Nyman
authors.forename: A
authors.initials: A
authors.lastname: Wallin