title: PCR cloning of a protein-coding part of the thioredoxin gene fromStreptomyces aureofaciens.
abstract: The method of two-stage half-specific amplification was described and successfully used in the isolation of the protein-coding part of the thioredoxin gene from Streptomyces aureofaciens BMK. The efficiency of a new PCR modification for the specific amplification of the target DNA fragments (genes) with unknown sequences is compared with the used half-specific PCR. The determined target sequence demonstrates the highest homology with the thioredoxin genes from Corynebacterium nephridii C-1 and Anabaena 7119.
authors.affiliation: Department of Biochemistry, Faculty of Science, Comenius University, Bratislava, Slovakia.
authors.forename: O
authors.initials: O
authors.lastname: Labudová
authors.forename: M
authors.initials: M
authors.lastname: Némethová
authors.forename: M
authors.initials: M
authors.lastname: Kollárová
authors.forename: T
authors.initials: T
authors.lastname: Skern
authors.forename: E
authors.initials: E
authors.lastname: Küchler