title: Water-mediated structuring of bone apatite.
abstract: It is well known that organic molecules from the vertebrate extracellular matrix of calcifying tissues are essential in structuring the apatite mineral. Here, we show that water also plays a structuring role. By using solid-state nuclear magnetic resonance, wide-angle X-ray scattering and cryogenic transmission electron microscopy to characterize the structure and organization of crystalline and biomimetic apatite nanoparticles as well as intact bone samples, we demonstrate that water orients apatite crystals through an amorphous calcium phosphate-like layer that coats the crystalline core of bone apatite. This disordered layer is reminiscent of those found around the crystalline core of calcified biominerals in various natural composite materials in vivo. This work provides an extended local model of bone biomineralization.
authors.affiliation: 1] Laboratoire Chimie de la Matière Condensée de Paris, UMR 7574 CNRS, UPMC Univ Paris 06, Collège de France, 11 place Marcelin Berthelot, 75231 Paris Cedex 05, France [2].
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Stanislas
authors.initials: S
authors.lastname: Von Euw
authors.forename: Francisco M
authors.initials: FM
authors.lastname: Fernandes
authors.forename: Sophie
authors.identifier: 0000000165993718
authors.initials: S
authors.lastname: Cassaignon
authors.forename: Mohamed
authors.initials: M
authors.lastname: Selmane
authors.forename: Guillaume
authors.identifier: 0000000281275326
authors.initials: G
authors.lastname: Laurent
authors.forename: Gérard
authors.initials: G
authors.lastname: Pehau-Arnaudet
authors.forename: Cristina
authors.initials: C
authors.lastname: Coelho
authors.forename: Laure
authors.initials: L
authors.lastname: Bonhomme-Coury
authors.forename: Marie-Madeleine
authors.initials: MM
authors.lastname: Giraud-Guille
authors.forename: Florence
authors.initials: F
authors.lastname: Babonneau
authors.forename: Thierry
authors.initials: T
authors.lastname: Azaïs
authors.forename: Nadine
authors.initials: N
authors.lastname: Nassif
mesh_terms: D000327:Adsorption; D001031:Apatites / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D032701:Biomimetics; D001842:Bone and Bones / Q000737:chemistry*; D002113:Calcification, Physiologic; D002130:Calcium Phosphates / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008422:Materials Testing; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry; D012542:Scattering, Radiation; D013499:Surface Properties; D013696:Temperature; D013818:Thermogravimetry; D014867:Water / Q000737:chemistry*; D014965:X-Rays
chemical_list: D001031:Apatites; D001672:Biocompatible Materials; D002130:Calcium Phosphates; C519480:amorphous calcium phosphate; D014867:Water