title: The phylogenetic position of Anaplasma bovis and inferences on the phylogeny of the genus Anaplasma.
abstract: The present study aimed to determine the complete citrate synthase (gltA) and heat-shock protein (groEL) gene sequences of Anaplasma bovis and to infer phylogenetic relationships within the genus Anaplasma. Multiple alignments from single and concatenated sequences of the 16S rRNA, gltA and groEL genes of the genus Anaplasma were subjected to phylogenetic analyses. Percent identities of A. bovis nucleotide sequences were found highest with A. phagocytophilum in gltA (65.4%) and groEL (79.8%). Single gene phylogenetic tree results assumed similar phylogenetic positions within the genus Anaplasma, except for A. bovis. However, consensus and concatenated sequence phylogenetic trees showed similar results, revealing 2 subgroups within the genus.
authors.affiliation: Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Adrian Patalinghug
authors.initials: AP
authors.lastname: Ybañez
authors.forename: Mariko
authors.initials: M
authors.lastname: Sashika
authors.forename: Hisashi
authors.initials: H
authors.lastname: Inokuma
mesh_terms: D000709:Anaplasma / Q000145:classification / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D001499:Bayes Theorem; D018834:Chaperonin 60 / Q000235:genetics; D002950:Citrate (si)-Synthase / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D016013:Likelihood Functions; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D017931:DNA Primers; D012336:RNA, Ribosomal, 16S; D002950:Citrate (si)-Synthase