title: The genetic characterisation of novel multi-addition doubled haploid lines derived from triticale x wheat hybrids.
abstract: Two novel 46-chromosome doubled haploid lines, W66 and M17, derived from separate hexaploid triticale x bread wheat crosses, were characterised using cytological and biochemical markers. Both lines were shown to be relatively stable cytologically, over 11 and 8 generations of selfing, respectively. By examining mitotic and meiotic chromosomes, the stabilities of the two lines were shown to be similar with frequencies of 2n=46 in 74.2-85.5% of cells. However, over selfed generations, the rye chromosomes were shown to have lost some of their heterochromatin, which made it difficult to establish their continued presence using cytological techniques, such as C-banding alone. Cytological evidence from pairing studies, C-banding, and fluorescence in-situ hybridization, showed that both M17 and W66 are wheat/rye multi-addition lines with rye chromosome constitutions of 1R+6R, and 1R+4R, respectively. These conclusions were confirmed by isozyme and storage-protein analysis.
authors.affiliation: Institute of Genetics, Academia Sinica, 100101, Beijing, China.
authors.forename: G
authors.initials: G
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Ji
authors.forename: Y B
authors.initials: YB
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Hu
authors.forename: I P
authors.initials: IP
authors.lastname: King
authors.forename: J W
authors.initials: JW
authors.lastname: Snape