title: Kinetic studies of peptide bond formation. Effect of chloramphenicol.
abstract: There might be an undetermined order in the interaction of the substrates with the ribosomes in the reaction of CACCA-Leu-Ac with puromycin to form Ac-Leu-puromycin and CACCA ('fragment reaction'). Km 0°=6×10(-4) M for the puromycin · ribosome interaction. Chloramphenicol totally blocks the 'fragment reaction' as a consequence of a single interaction with the ribosome of Kd 0°=2.2×10(-6) M. The inhibition by chloramphenicol of the 'fragment reaction' is mixed competitive for puromycin.
authors.affiliation: Instituto de Biología Celular, Madrid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Fernandez-Muñoz
authors.forename: D
authors.initials: D
authors.lastname: Vazquez