title: Higher TGF-β with lower CD124 and TSLP, but no difference in PAR-2 expression in bronchial biopsy of bronchial asthma patients in comparison with COPD patients.
abstract: Chronic obstructive pulmonary disease (COPD) and bronchial asthma (BA) are 2 severe respiratory disorders with different predominated immunopathologies. There are several "novel molecules" from different families that are proposed as part of the etiopathogenesis of COPD and BA. Proteinase-activated receptor 2 (PAR-2), thymic stromal lymphoprotein (TSLP), interleukin-4 and its receptor (CD124), Yin-Yang 1 (YY1), and transforming growth factor beta (TGF-β) have been previously shown to be involved in the pathophysiology of both these diseases. We investigated PAR-2, TSLP, CD124 (interleukin-4R), TGF-β, and YY1 immunohistochemical expression in endobronchial and transbronchial biopsies from 22 BA patients and 20 COPD patients. Immunostaining for the above-mentioned antigens was quantified using a modified semiquantitative scoring system and statistically evaluated. The values of TGF-β in the epithelial cells (P=0.0007) and TGF-β in the submucosa (P=0.0075) were higher in the BA samples, whereas values of CD124 (P=0.0015) and TSLP (P=0.0106) were higher in the COPD samples. No statistically significant differences between the groups were recorded for PAR-2 and YY1. Airway inflammatory reaction diversity in BA and COPD seems to be disease specific; however, there are also shared mechanisms involved in the pathophysiology of both diseases.
authors.affiliation: Departments of *Pathology and Molecular Medicine ‡Respiratory Diseases, Thomayer Teaching Hospital †Department of Pathology, Third Faculty of Medicine, Charles University §Faculty of Nuclear Sciences and Physical Engineering, Czech Technical University ∥Department No. 75, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Radoslav
authors.initials: R
authors.lastname: Matěj
authors.forename: Martina
authors.initials: M
authors.lastname: Vašáková
authors.forename: Jaromír
authors.initials: J
authors.lastname: Kukal
authors.forename: Martina
authors.initials: M
authors.lastname: Sterclová
authors.forename: Tomáš
authors.initials: T
authors.lastname: Olejár
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001249:Asthma / Q000378:metabolism / Q000473:pathology; D001706:Biopsy; D001980:Bronchi / Q000378:metabolism / Q000473:pathology; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053662:Interleukin-4 Receptor alpha Subunit / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000378:metabolism / Q000473:pathology; D044464:Receptor, PAR-2 / Q000096:biosynthesis*; D016212:Transforming Growth Factor beta / Q000096:biosynthesis*; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D016207:Cytokines; C504000:IL4R protein, human; D053662:Interleukin-4 Receptor alpha Subunit; D044464:Receptor, PAR-2; D016212:Transforming Growth Factor beta; D000094632:Thymic Stromal Lymphopoietin