title: Elicitor-mediated induction of isochorismate synthase and accumulation of 2,3-dihydroxy benzoic acid in Catharanthus roseus cell suspension and shoot cultures.
abstract: After elicitation, cell suspension cultures of Catharanthus roseus accumulate phenolic compounds. The major phenolic compound produced was isolated and identified as 2,3-dihydroxybenzoic acid (DHBA). The accumulation of this compound is a rapid response to the addition of elicitor; within 6 h after the addition of elicitor, DHBA concentration reached 6.3 mg/l cell suspension. DHBA was not detected in non-elicited cells. The formation of DHBA in elicited cells was correlated with the induction of the enzyme isochorismate synthase (ICS). Shoot cultures of C. roseus also presented a strong induction of ICS after elicitation. Due to its biological activity, DHBA could play a role in the defence mechanism of C. roseus.
authors.affiliation: Leiden/Amsterdam Centre for Drug Research, Division of Pharmacognosy, Gorlaeus Laboratoria, Leiden University, P.O. Box 9502, 2300, RA - Leiden, The Netherlands.
authors.forename: P R
authors.initials: PR
authors.lastname: Moreno
authors.forename: R
authors.initials: R
authors.lastname: van der Heijden
authors.forename: R
authors.initials: R
authors.lastname: Verpoorte