title: The use of quorum sensing to improve vaccine immune response.
abstract: Enterotoxigenic Escherichia coli (ETEC) infection is an important cause of diarrhea in both newborn and post-weaning pigs, it is also responsible for economic losses on farms worldwide. Vaccines that use ETEC virulence factors have been well documented, and several vaccines containing inactivated bacteria with protective antigens, or purified (isolated) antigens are available on the market. Vaccination of pregnant sows is widely seen as an effective strategy for the control of the disease. Yet these vaccines very often do not lead to efficient protection. In this study, we produced an ETEC bacterin with the use of quorum sensing (QS), and observed a significant expression of F4 adhesin, and heat-labile toxin (LT) in the cultures when compared to the controls. Mice, and pigs vaccinated with the QS bacterin demonstrated higher antibody titers against these antigens when compared with commercial and control bacterin. Our results suggest that the system might bring promising improvements in ETEC bacterin efficacy.
authors.affiliation: CDTec-Biotechnology, Federal University of Pelotas, RS, Brazil.
authors.forename: R T
authors.initials: RT
authors.lastname: Sturbelle
authors.affiliation: CDTec-Biotechnology, Federal University of Pelotas, RS, Brazil.
authors.forename: R C S
authors.initials: RC
authors.lastname: Conceição
authors.affiliation: CDTec-Biotechnology, Federal University of Pelotas, RS, Brazil.
authors.forename: M C
authors.initials: MC
authors.lastname: Da Rosa
authors.affiliation: Federal University of Pará, PA, Brazil.
authors.forename: T B
authors.initials: TB
authors.lastname: Roos
authors.affiliation: CDTec-Biotechnology, Federal University of Pelotas, RS, Brazil.
authors.forename: L
authors.initials: L
authors.lastname: Dummer
authors.affiliation: CDTec-Biotechnology, Federal University of Pelotas, RS, Brazil. Electronic address: fabio_leite@ufpel.com.br.
authors.forename: F P L
authors.initials: FP
authors.lastname: Leite
mesh_terms: D000820:Animal Diseases / Q000276:immunology* / Q000517:prevention & control*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology; D054307:Enterotoxigenic Escherichia coli / Q000276:immunology*; D004768:Enterotoxins / Q000235:genetics / Q000276:immunology; D004927:Escherichia coli Infections / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000276:immunology; D022361:Escherichia coli Vaccines / Q000235:genetics / Q000276:immunology*; D005260:Female; D010861:Fimbriae, Bacterial / Q000235:genetics; D051379:Mice; D053038:Quorum Sensing / Q000235:genetics / Q000276:immunology*; D013552:Swine
chemical_list: D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D022361:Escherichia coli Vaccines; C027538:heat-labile enterotoxin, E coli
keywords: Autoinducer; Bacterin; Enterotoxigenic Escherichia coli; Epinephrine