title: In vitro propagation of Clerodendrum colebrookianum Walp., a potential natural anti-hypertension medicinal plant.
abstract: A rapid clonal propagation system for Clerodendrum colebrookianum Walp. (Verbenaceae), a anti-hypertension folk medicinal shrub has been developed. A range of cytokinins has been investigated for multiple shoot induction with shoot apex, axillary shoot, leaf, petiole and root explants. Optimum shoot induction occurred with axillary buds using 6-benzyladenine where an average of 21 shoots were produced per explant in 6 weeks. Subculturing the newly produced shoots, by separating into groups of five shoots, produced an average of 43 new shoots per culture within 4 weeks. In vitro rooting and weaning of over 200 plantlets was completely successful. Cytological studies revealed no visible abnormalities in chromosome number.
authors.affiliation: Department of Agricultural Botany, School of Plant Sciences, The University of Reading, Whiteknights, P.O. Box 221, RG6 2AS, Reading, UK.
authors.forename: A A
authors.initials: AA
authors.lastname: Mao
authors.forename: A
authors.initials: A
authors.lastname: Wetten
authors.forename: M
authors.initials: M
authors.lastname: Fay
authors.forename: P D
authors.initials: PD
authors.lastname: Caligari