title: RFLP analysis of highly polymorphic loci in barley.
abstract: Barley middle-repeat sequences were screened for their ability to discriminate 51 barley commercial varieties. Two hordein clones, a clone encoding a leaf-specific thionin, a desiccation induced cDNA clone, a clone coding for 5S-rRNA and one corresponding to ubiquitin genes were tested. A very sensitive RFLP technique including four cutter restriction enzymes and denaturing 4% polyacrylamide gels were used to evidence the highest level of polymorphism.The RFLP data were analyzed by computer. Some probe/enzyme combinations were able to differentiate a large number of the cultivars tested, whereas three probe/enzyme combinations succeeded in identifying all the varieties. The use of this RFLP method can thus be suggested for cultivar identification in barley.
authors.affiliation: Experimental Institute for Cereal Research, I-29017 Fiorenzuola d'Arda (PC), 302, Via S. Protaso, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Pecchioni
authors.forename: A M
authors.initials: AM
authors.lastname: Stanca
authors.forename: V
authors.initials: V
authors.lastname: Terzi
authors.forename: L
authors.initials: L
authors.lastname: Cattivelli