title: Quantification of metallothionein on the liver and kidney of rats by subchronic lead and cadmium in combination.
abstract: The combined subchronic effects of exposure to lead acetate and cadmium chloride on oxidative stress and metallothionein (MT) gene expression were detected in the liver and kidney of rats to investigate the hazards of environmentally relevant, low-dose exposure to these compounds. Pb and Cd co-induced oxidative stress in liver and kidney tissues. This result was indicated by a significant (P<0.01) increase in the maleic dialdehyde level and decreased levels of reduced glutathione, superoxide dismutase, catalase, and glutathione peroxidase. MT mRNA and protein significantly increased (P<0.01) in the liver and kidney of rats. Furthermore, the expression levels of MT-1 mRNA and MT-2 mRNA differed between the liver and kidney. The findings indicate that Pb combined with Cd induced oxidative damage in the liver and kidney of rats, and MT may be a biochemical environmental indicator.
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Dai
authors.forename: Zhongqiong
authors.initials: Z
authors.lastname: Yin
authors.forename: Guiping
authors.initials: G
authors.lastname: Yuan
authors.forename: Hongke
authors.initials: H
authors.lastname: Lu
authors.forename: Renyong
authors.initials: R
authors.lastname: Jia
authors.forename: Jiao
authors.initials: J
authors.lastname: Xu
authors.forename: Xu
authors.initials: X
authors.lastname: Song
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Shu
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Liang
authors.forename: Changliang
authors.initials: C
authors.lastname: He
authors.forename: Cheng
authors.initials: C
authors.lastname: Lv
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002104:Cadmium / Q000378:metabolism / Q000633:toxicity*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007150:Immunohistochemistry; D007202:Indicators and Reagents; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism; D007854:Lead / Q000378:metabolism / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008668:Metallothionein / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000975:Antioxidants; D007202:Indicators and Reagents; D012333:RNA, Messenger; D002104:Cadmium; D007854:Lead; D008668:Metallothionein
keywords: Cd; Metallothionein; Oxidative stress; Pb; Subchronic