title: Sgol2 provides a regulatory platform that coordinates essential cell cycle processes during meiosis I in oocytes.
abstract: Accurate chromosome segregation depends on coordination between cohesion resolution and kinetochore-microtubule interactions (K-fibers), a process regulated by the spindle assembly checkpoint (SAC). How these diverse processes are coordinated remains unclear. We show that in mammalian oocytes Shugoshin-like protein 2 (Sgol2) in addition to protecting cohesin, plays an important role in turning off the SAC, in promoting the congression and bi-orientation of bivalents on meiosis I spindles, in facilitating formation of K-fibers and in limiting bivalent stretching. Sgol2's ability to protect cohesin depends on its interaction with PP2A, as is its ability to silence the SAC, with the latter being mediated by direct binding to Mad2. In contrast, its effect on bivalent stretching and K-fiber formation is independent of PP2A and mediated by recruitment of MCAK and inhibition of Aurora C kinase activity respectively. By virtue of its multiple interactions, Sgol2 links many of the processes essential for faithful chromosome segregation. DOI: http://dx.doi.org/10.7554/eLife.01133.001.
authors.affiliation: Department of Biochemistry, University of Oxford, Oxford, United Kingdom.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Rattani
authors.forename: Magda
authors.initials: M
authors.lastname: Wolna
authors.forename: Mickael
authors.initials: M
authors.lastname: Ploquin
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Helmhart
authors.forename: Seamus
authors.initials: S
authors.lastname: Morrone
authors.forename: Bernd
authors.initials: B
authors.lastname: Mayer
authors.forename: Jonathan
authors.initials: J
authors.lastname: Godwin
authors.forename: Wenqing
authors.initials: W
authors.lastname: Xu
authors.forename: Olaf
authors.initials: O
authors.lastname: Stemmann
authors.forename: Alberto
authors.initials: A
authors.lastname: Pendas
authors.forename: Kim
authors.initials: K
authors.lastname: Nasmyth
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D002503:Centromere; D006801:Humans; D018386:Kinetochores; D008969:Molecular Sequence Data; D011485:Protein Binding; D054648:Protein Phosphatase 2 / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D018797:Cell Cycle Proteins; C510590:SGO2 protein, human; D054648:Protein Phosphatase 2
keywords: Aurora kinase; Chromosome segregation; MCAK; Meiosis; PP2A; Shugoshin