title: miR-29a suppresses growth and invasion of gastric cancer cells in vitro by targeting VEGF-A.
abstract: Increasing data shows miR-29a is a key regulator of oncogenic processes. It is significantly down-regulated in some kind of human tumors and possibly functionally linked to cellular proliferation, survival and migration. However, the mechanism remains unclear. In this study, we report miR-29a is significantly under-expressed in gastric cancer compared to the healthy donor. The microvessel density is negatively related to miR-29a expression in gastric cancer tissues. The ectopic expression of miR-29a significantly inhibits proliferation and invasion of gastric cancer cells. Furthermore, western blot combined with the luciferase reporter assays demonstrate that vascular endothelial growth factor A (VEGF-A) is direct target of miR-29a. This is the first time miR-29a was found to suppress the tumor microvessel density in gastric cancer by targeting VEGF-A. Taken together, these results suggest that miR-29a is a tumor suppressor in gastric cancer. Restoration of miR-29a in gastric cancer may be a promising therapeutic approach.
authors.affiliation: Department of Gastroenterology, the 324 th Hospital of PLA, Chongqing 400020, China lingcong_008@126.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Hong
authors.initials: H
authors.lastname: Xiao
authors.forename: Zong-Hua
authors.initials: ZH
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Huang
authors.forename: Zi-Peng
authors.initials: ZP
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Ren
authors.forename: Hang
authors.initials: H
authors.lastname: Song
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D055806:Microvessels / Q000502:physiology; D009362:Neoplasm Metastasis; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A