title: Recovery of plants from cryopreserved embryogenic cell suspensions of Pinus caribaea.
abstract: Embryogenic cell suspension cultures of Pinus caribaea var. hondurensis have been cryopreserved in liquid nitrogen for up to four months, using sucrose and dimethylsulfoxide as cryoprotectants. Post-thaw growth was obtained after a short lag phase. Removal of the remaining liquid around the cells using a filter disc favoured subsequent regrowth of the cells. These reestablished cultures maintained an embryogenic potential similar to non-frozen cultures. The embryos produced were able to regenerate into plants, which are now growing in a greenhouse.
authors.affiliation: Laboratoire de Biotechnologies Végétales, Université de Picardie, Faculté des Sciences, 33, rue Saint-Leu, 80039, Amiens Cedex, France.
authors.forename: E
authors.initials: E
authors.lastname: Laine
authors.forename: P
authors.initials: P
authors.lastname: Bade
authors.forename: A
authors.initials: A
authors.lastname: David