title: Astilbin protects diabetic rat heart against ischemia-reperfusion injury via blockade of HMGB1-dependent NF-κB signaling pathway.
abstract: Astilbin, a flavonoid compound was isolated from the rhizome of Smilax china L. In this study, we investigated the anti-myocardial ischemia and reperfusion (I/R) injury effect of Astilbin on diabetic rats in vivo and elucidated the potential mechanism in vitro. The results showed that Astilbin significantly attenuated hypoxia-induced cell injury in a concentration-dependent manner. Treatment of H9c2 cells with Astilbin at 15 μM blocked nuclear factor kappaB (NF-κB) phosphorylation by blocking High-mobility group box protein 1 (HMGB1) expression. Treatment of diabetic rats with Astilbin by intravenous injection (i.v.) at a single dose of 50 mg/kg protected the rats from myocardial I/R injury as indicated by decreasing infarct volume, improving hemodynamics and reducing myocardial damage, and also lowered serum levels of pro-inflammatory factors, reduced HMGB1 and phosphorylated NF-κB expression in ischemic myocardial tissue from diabetic rats. Additionally, treatment of diabetic rats with Astilbin at dose of 50 mg/kg by i.v. for continuous 14 days attenuated cardiac remodeling in the model myocardial I/R injury. These protective effects suggested that Astilbin might be due to block of the myocardial inflammatory cascade via the HMGB1-dependent NF-κB signaling pathway.
authors.affiliation: School of Pharmaceutical Sciences, Binzhou Medical University, Yantai 264003, PR China.
authors.forename: Huiling
authors.initials: H
authors.lastname: Diao
authors.affiliation: School of Pharmaceutical Sciences, Binzhou Medical University, Yantai 264003, PR China.
authors.forename: ZeChun
authors.initials: Z
authors.lastname: Kang
authors.affiliation: School of Pharmaceutical Sciences, Binzhou Medical University, Yantai 264003, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Han
authors.affiliation: School of Pharmaceutical Sciences, Binzhou Medical University, Yantai 264003, PR China. Electronic address: jwl518@163.com.
authors.forename: Wanglin
authors.initials: W
authors.lastname: Jiang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D044948:Flavonols / Q000494:pharmacology*; D024243:HMGB1 Protein / Q000502:physiology*; D006321:Heart / Q000187:drug effects*; D018836:Inflammation Mediators / Q000097:blood; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D016328:NF-kappa B / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D044948:Flavonols; D024243:HMGB1 Protein; C104200:Hbp1 protein, rat; D018836:Inflammation Mediators; D016328:NF-kappa B; C099069:astilbin
keywords: Astilbin; Diabetes mellitus; High mobility group box-1 protein; Myocardial ischemia and reperfusion; Nuclear factor kappaB