title: Crystallization and preliminary X-ray study of alanine dehydrogenase from Bacillus pseudofirmus OF4.
abstract: Alanine dehydrogenase (OF4Ald) from the alkaliphilic Bacillus pseudofirmus OF4 was expressed and purified with a His6 tag in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffusion method at 289 K using a solution consisting of 0.1 M Tris-HCl pH 8.0, 0.2 M LiSO4, 22%(w/v) PEG 3350. X-ray diffraction data were collected to 2.8 Å resolution. The crystal belonged to the triclinic space group P1, with unit-cell parameters a = 88.04, b = 105.59, c = 120.53 Å, α = 88.37, β = 78.77, γ = 82.65°.
authors.affiliation: Tianjin University of Science and Technology, Tianjin 300222, People's Republic of China.
authors.forename: Jinjin
authors.initials: J
authors.lastname: Wen
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Li
authors.forename: Guangzheng
authors.initials: G
authors.lastname: He
authors.forename: Shujing
authors.initials: S
authors.lastname: Xu
authors.forename: Baohua
authors.initials: B
authors.lastname: Zhao
authors.forename: Xianming
authors.initials: X
authors.lastname: Zhu
authors.forename: Hui
authors.initials: H
authors.lastname: Dong
authors.forename: Jiansong
authors.initials: J
authors.lastname: Ju
mesh_terms: D050788:Alanine Dehydrogenase / Q000737:chemistry*; D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D016415:Sequence Alignment
chemical_list: D001426:Bacterial Proteins; D050788:Alanine Dehydrogenase
keywords: Bacillus pseudofirmus; alanine dehydrogenase