title: A comparison of Ypet and firefly luciferase as reporter proteins for high-throughput screening.
abstract: When Ypet was used as a reporter protein for high-throughput screening (HTS), it showed peak fold induction and a dynamic range similar to those for firefly luciferase. We also determined that conducting a reading immediately after media aspiration was the best method for HTS. We conclude that Ypet can serve as a substitute for luciferase as a reporter protein in HTS assays.
authors.affiliation: College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University.
authors.forename: Sei Mee
authors.initials: SM
authors.lastname: Yoon
authors.forename: Wan
authors.initials: W
authors.lastname: Namkung
authors.forename: Jinu
authors.initials: J
authors.lastname: Lee
mesh_terms: D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D057809:HEK293 Cells; D057166:High-Throughput Screening Assays; D006801:Humans; D007202:Indicators and Reagents; D049409:Luciferases, Firefly / Q000235:genetics* / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D013539:Simian virus 40 / Q000737:chemistry / Q000235:genetics
chemical_list: D007202:Indicators and Reagents; D016328:NF-kappa B; D020033:Protein Isoforms; C485192:green fluorescent protein, Aequorea victoria; D049452:Green Fluorescent Proteins; D049409:Luciferases, Firefly