title: Fast method for the simultaneous quantification of toxic polyphenols applied to the selection of genotypes of yam bean (Pachyrhizus sp.) seeds.
abstract: The purpose of the research was to develop and validate a rapid quantification method able to screen many samples of yam bean seeds to determine the content of two toxic polyphenols, namely pachyrrhizine and rotenone. The analytical procedure described is based on the use of an internal standard (dihydrorotenone) and is divided in three steps: microwave assisted extraction, purification by solid phase extraction and assay by ultra high performance liquid chromatography (UHPLC). Each step was included in the validation protocol and the accuracy profiles methodology was used to fully validate the method. The method was fully validated between 0.25 mg and 5 mg pachyrrhizin per gram of seeds and between 0.58 mg/g and 4 mg/g for rotenone. More than one hundred samples from different accessions, locations of growth and harvest dates were screened. Pachyrrhizine concentrations ranged from 3.29 mg/g to lower than 0.25 mg/g while rotenone concentrations ranged from 3.53 mg/g to lower than 0.58 mg/g. This screening along with principal component analysis (PCA) and discriminant analysis (DA) analyses allowed the selection of the more interesting genotypes in terms of low concentrations of these two toxic polyphenols.
authors.affiliation: Laboratoire de Pharmacognosie, LDRI, Avenue E. Mounier, 72 - B1 7203 Université catholique de Louvain, 1200 Brussels, Belgium. Electronic address: emmanuelle.lautie@uclouvain.be.
authors.forename: E
authors.initials: E
authors.lastname: Lautié
authors.forename: E
authors.initials: E
authors.lastname: Rozet
authors.forename: P
authors.initials: P
authors.lastname: Hubert
authors.forename: N
authors.initials: N
authors.lastname: Vandelaer
authors.forename: F
authors.initials: F
authors.lastname: Billard
authors.forename: T Zum
authors.initials: TZ
authors.lastname: Felde
authors.forename: W J
authors.initials: WJ
authors.lastname: Grüneberg
authors.forename: J
authors.initials: J
authors.lastname: Quetin-Leclercq
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016002:Discriminant Analysis; D005838:Genotype; D057230:Limit of Detection; D008872:Microwaves; D029905:Pachyrhizus / Q000737:chemistry* / Q000235:genetics; D059808:Polyphenols / Q000032:analysis*; D025341:Principal Component Analysis; D012015:Reference Standards; D012402:Rotenone / Q000031:analogs & derivatives / Q000032:analysis*; D012639:Seeds / Q000737:chemistry* / Q000235:genetics; D052616:Solid Phase Extraction; D013997:Time Factors
chemical_list: D059808:Polyphenols; D012402:Rotenone; C044985:1',2'-dihydrorotenone
keywords: Accuracy profile; Dihydrorotenone synthesis; Furocoumarin; Isoflavonoid; PCA–DA; UHPLC-UV