title: Isolation and identification of methanethiol-utilizing bacterium CZ05 and its application in bio-trickling filter of biogas.
abstract: A bacterium capable of methanethiol (MT) degradation was enriched and isolated by employing activated sewage sludge as the inoculum in a mineral medium containing MT. The isolate was identified as Paenibacillus polymyxa CZ05 through a Biolog test and 16S rDNA sequencing. This strain can utilize both organic and inorganic media and thrives at pH 4 to 9. The batch culture showed that the strain can degrade MT better in the No. 4 medium than in the No. 1 medium. A series-operating biotrickling filter with lava stone as the carrier was employed to test the application of P. polymyxa CZ05 in the removal of MT in simulated biogas. Long-term experiments showed that a high concentration of MT (60 ppm) was efficiently removed (99.5%) by the biotrickling filters at EBRT 30 s. The addition of hydrogen sulfide decreased the MT removal rate because the dissolved oxygen competed with MT.
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China; Key Laboratory of Ministry of Education Industrial Fermentation Microbiology, Tianjin Key Laboratory of Industrial Microbiology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors.forename: Chao-zheng
authors.initials: CZ
authors.lastname: Zhang
authors.forename: Wei-jiang
authors.initials: WJ
authors.lastname: Zhang
authors.forename: Jiao
authors.initials: J
authors.lastname: Xu
mesh_terms: D001673:Biodegradation, Environmental / Q000187:drug effects; D056804:Biofuels / Q000382:microbiology*; D019149:Bioreactors / Q000382:microbiology*; D057075:Enzyme Assays; D005285:Fermentation / Q000187:drug effects; D005374:Filtration / Q000295:instrumentation*; D006862:Hydrogen Sulfide / Q000494:pharmacology; D056507:Paenibacillus / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism*
chemical_list: D056804:Biofuels; D013438:Sulfhydryl Compounds; C005231:methylmercaptan; D006862:Hydrogen Sulfide
keywords: Biotrickling filter; Identification; Isolation; Methanethiol; Paenibacillus polymyxa