title: Amidase encapsulated O-carboxymethyl chitosan nanoparticles for vaccine delivery.
abstract: This work reports the development of amidase encapsulated O-carboxymethyl chitosan nanoparticles (Ami-O-CMC NPs) of 300±50 nm size by ionic cross-linking method. The prepared Ami-O-CMC NPs had an encapsulation efficiency of 55.39%. Haemolysis assay and cytotoxicity studies proved the hemocompatibility and cytocompatibility of the prepared NPs. The sustained release of Ami from the NPs is expected to prolong its immunogenicity and in turn lead to development of better protective immunity against Staphylococcus aureus infections.
authors.affiliation: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi 682041, India.
authors.forename: K T
authors.initials: KT
authors.lastname: Smitha
authors.affiliation: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi 682041, India.
authors.forename: M
authors.initials: M
authors.lastname: Sreelakshmi
authors.affiliation: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi 682041, India.
authors.forename: N
authors.initials: N
authors.lastname: Nisha
authors.affiliation: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi 682041, India.
authors.forename: R
authors.initials: R
authors.lastname: Jayakumar
authors.affiliation: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi 682041, India. Electronic address: rajabiswas@aims.amrita.edu.
authors.forename: Raja
authors.initials: R
authors.lastname: Biswas
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry*; D048271:Chitosan / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D016503:Drug Delivery Systems; D006801:Humans; D016233:Immunotherapy, Active; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology / Q000473:pathology; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: C504164:O-carboxymethylchitosan; D048271:Chitosan; D000581:Amidohydrolases; C019665:amidase
keywords: Amidase encapsulation; Immunogenicity; Staphylococcus aureus