title: Comparison of frozen and unfrozen blood spots for gene expression studies.
abstract: We studied gene expression in 9 sets of paired newborn blood spots stored for 8-10 years in either the frozen state or the unfrozen state. Fewer genes were expressed in unfrozen spots, but the average correlation coefficient for overall gene expression comparing the frozen and unfrozen state was 0.771 (95% CI, 0.700-0.828).
authors.affiliation: Department of Epidemiology and Biostatistics, College of Human Medicine, Michigan State University, East Lansing, MI.
authors.forename: Changshuai
authors.initials: C
authors.lastname: Wei
authors.affiliation: Department of Epidemiology and Biostatistics, College of Human Medicine, Michigan State University, East Lansing, MI.
authors.forename: Qing
authors.initials: Q
authors.lastname: Lu
authors.affiliation: Laboratory of Microarray Technology, Van Andel Research Institute, Grand Rapids, MI.
authors.forename: Sok Kean
authors.initials: SK
authors.lastname: Khoo
authors.affiliation: Department of Epidemiology and Biostatistics, College of Human Medicine, Michigan State University, East Lansing, MI.
authors.forename: Madeleine
authors.initials: M
authors.lastname: Lenski
authors.affiliation: Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School and Brigham and Women's Hospital, Boston, MA.
authors.forename: Raina N
authors.initials: RN
authors.lastname: Fichorova
authors.affiliation: Neuroepidemiology Unit, Department of Neurology, Boston Children's Hospital, Harvard University, Boston, MA.
authors.forename: Alan
authors.initials: A
authors.lastname: Leviton
authors.affiliation: Department of Epidemiology and Biostatistics, College of Human Medicine, Michigan State University, East Lansing, MI; Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI. Electronic address: paneth@msu.edu.
authors.forename: Nigel
authors.initials: N
authors.lastname: Paneth
mesh_terms: D001800:Blood Specimen Collection; D015925:Cryopreservation; D005615:Freezing; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007231:Infant, Newborn; D015997:Neonatal Screening; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000097:blood*; D013997:Time Factors
chemical_list: D012333:RNA, Messenger
keywords: ELGAN; Extremely Low Gestational Age Newborns