title: Isolation and characterization of mitochondrial DNA from the alkane yeast Saccharomycopsis lipolytica.
abstract: Mitochondrial (mt) DNA of the alkane yeast, Saccharomycopsis lipolytica, was isolated. Its buoyant density in CsCl was found to be of 1.687 g/cm(3), indicating a GC content of 27.5% and its melting point Tm = 79.5 °C, indicating a GC content of 24.9%. The corresponding values for nuclear (n) DNA, are 1.709 g/cm(3) (GC: 49.5%) and Tm = 90.5 (GC: 51.7%) respectively. Electron microscopy revealed that mtDNA has a circular structure with a contour length of about 14.5 µm corresponding to 45.5 kb per molecule. The size estimated from restriction analyses performed with 7 endonucleases was 48.35 kb/molecule. A restriction map was constructed, using the cleavage data of 4 endonucleases.
authors.affiliation: Lehrstuhl für Allgemeine Botanik, Ruhr-Universität, Postfach 102148, D-4630, Bochum 1, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Kück
authors.forename: U
authors.initials: U
authors.lastname: Stahl
authors.forename: A
authors.initials: A
authors.lastname: Lhermitte
authors.forename: K
authors.initials: K
authors.lastname: Esser