title: Plant regeneration from protoplasts of long-term callus cultures of Actinidia deliciosa var. deliciosa cv. Hayword (Kiwifruit).
abstract: Plant regeneration of Actinidia deliciosa var. deliciosa cv. Hayword was obtained from protoplasts isolated from petiole derived long-term callus cultures. Protoplasts were cultured in liquid medium over agarose gelled medium. Regenerated green callus, plated on solid medium, could develop shoots that rooted spontaneously in hormone-less medium. The plants obtained are growing fast in soil and present a normal phenotype.
authors.affiliation: Departamento de Biologia Vegetal, Faculdade de Ciências de Lisboa, Bloco C2, 1700, Campo Grande, Lisboa, Portugal.
authors.forename: M M
authors.initials: MM
authors.lastname: Oliveira
authors.forename: M S
authors.initials: MS
authors.lastname: Pais