title: Production of gynogenic haploids of Hordeum vulgare L.
abstract: Haploid plants were regenerated from cultured unfertilized ovaries of Hordeum vulgare L. (barley). Optimal response was obtained by the addition of 0.6 μM 4-chloro-2-methylphenoxyacetic acid (MCPA), 2.8 μM indole-3-acetic acid (IAA) and 4.4 μM 6-benzyladenine (BA) in the N6 medium. Further increase in the rate of callus formation and the number of green plants produced was possible with the addition of 90 g/l sucrose and 100 g/l coconut water. The stage of development of the ovaries at the time of culture was critical; the largest number of plants being produced by ovaries from flowers at the trinucleate stage of pollen.
authors.affiliation: Estación Experimental de Aula Dei, Ap. 202, Zaragoza, Spain.
authors.forename: A M
authors.initials: AM
authors.lastname: Castillo
authors.forename: L
authors.initials: L
authors.lastname: Cistué