title: [Simultaneous determination of six ingredients in Huoxiang Zhengqi oral liquid by UPLC].
abstract: OBJECTIVE
  To develop a UPLC method for the simultaneous determination of liquiritin, narirutin, hesperidin, ammonium glycyrrhetate, honokiol and magnolol in Huoxiang Zhengqi oral liquid.
  
  
  METHODS
  A Zorbax Eclipse C18 column was used with the mobile phase of acetonitrile and 0. 05% phosphate acid by gradient elution at the detection wavelength of 220 nm. The flow rate was 0.42 mL x min(-1) and the column temperature was 30 degrees C.
  
  
  RESULTS
  The calibration curves were linear in the ranges of 0.001 7-0.034, 0.003 4-0.068, 0.006 4-0.128, 0.012 8-0.256, 0.003 2-0.064, 0.006 4-0.128 microg, respectively. The average recoveries were 103.3%, 98.39%, 98.29%, 102.1%, 98.45%, 102.2% with RSDs of 2.1%,1.0%, 0.50%, 2.3%, 0.9%, 2.0%, respectively.
  
  
  CONCLUSIONS
  The UPLC method was simple, rapid and accurate, it could be used for quality control of Huoxiang Zhengqi oral liquid.
authors.affiliation: Chongqing Fuling Pharmaceutical Co., Ltd., Chongqing 408000, China. 351131754@qq.com
authors.forename: Jia-Wen
authors.initials: JW
authors.lastname: Yu
authors.forename: Kai-Ying
authors.initials: KY
authors.lastname: Deng
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.forename: Bi-Yue
authors.initials: BY
authors.lastname: Zhu
authors.forename: Hong-Ya
authors.initials: HY
authors.lastname: Liu
mesh_terms: D000284:Administration, Oral; D001713:Biphenyl Compounds / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004187:Disaccharides / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D044950:Flavanones / Q000737:chemistry; D005960:Glucosides / Q000737:chemistry; D006569:Hesperidin / Q000737:chemistry; D017705:Lignans / Q000737:chemistry; D019999:Pharmaceutical Solutions / Q000737:chemistry; D000498:Allyl Compounds; D010636:Phenols
chemical_list: D001713:Biphenyl Compounds; D004187:Disaccharides; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005960:Glucosides; D006569:Hesperidin; D017705:Lignans; D019999:Pharmaceutical Solutions; C516480:Huoxiang-zhengqi; C005498:magnolol; C500601:narirutin; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols; C512196:liquiritin