title: Changes in antioxidant capacity and colour associated with the formation of β-carotene epoxides and oxidative cleavage derivatives.
abstract: In this study HPLC-DAD-MS/MS was applied for the identification of compounds derived from (all-E)-β-carotene following epoxidation and oxidative cleavage. The consequences on the CIELAB colour parameters and antioxidant capacity (AC) were also evaluated. Five apocarotenoids, three secocarotenoids, seven Z isomers and two epoxides were detected as a result of the oxidative cleavage. Four epoxides and three Z isomers were detected as a consequence of the epoxidation reaction. Some compounds were detected for the first time as a result of oxidation reactions. Both treatments led to a marked decrease in b(∗) and Cab(∗) values, indicating that these colour parameters can be used for the rapid assessment of β-carotene oxidation. The oxidative cleavage of β-carotene resulted in increased capacity to both scavenge ABTS(+) and quench singlet oxygen. These results suggest that the study of the AC of these oxidative derivatives and their possible usefulness as food ingredients deserves further attention.
authors.affiliation: Department of Food Science, Faculty of Food Engineering, University of Campinas, Postal Code 13083-862 Campinas, Brazil.
authors.forename: Poliana D
authors.initials: PD
authors.lastname: Gurak
authors.forename: Adriana Z
authors.initials: AZ
authors.lastname: Mercadante
authors.forename: M L
authors.initials: ML
authors.lastname: González-Miret
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Heredia
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: Meléndez-Martínez
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003116:Color; D004852:Epoxy Compounds / Q000737:chemistry*; D005503:Food Additives / Q000737:chemistry; D007536:Isomerism; D010084:Oxidation-Reduction; D053719:Tandem Mass Spectrometry; D019207:beta Carotene / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D004852:Epoxy Compounds; D005503:Food Additives; D019207:beta Carotene
keywords: Antioxidants; Apocarotenoids; CIELAB; Carotenoid epoxides; Colour measurement; Epoxidation; Oxidation; Oxidative cleavage; Secocarotenoids