title: [Synthesis of diisooctyl adipate catalyzed by lipase-displaying Pichia pastoris whole-cell biocatalysts].
abstract: An enzyme-displaying yeast as a whole-cell biocatalyst is an alternative to immobilized enzyme, due to its low-cost preparation and simple recycle course. Here, lipase-displaying Pichia pastoris whole-cell was used as a biocatalyst to synthesize diisooctyl adipate in the non-aqueous system. The maximum productivity of diisooctyl adipate was obtained as 85.0% in a 10 mL reaction system. The yield could be reached as high as 97.8% when the reaction system was scaled up to 200 mL. The purity obtained is 98.2% after vacuum distillation. Thus, the lipase-displaying P. pastoris whole-cell biocatalyst was promising in commercial application for diisooctyl adipate synthesis in non-aqueous phase.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, Guangdong, China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
authors.forename: Zi
authors.initials: Z
authors.lastname: Jin
authors.forename: Ying
authors.initials: Y
authors.lastname: Lin
authors.forename: Suiping
authors.initials: S
authors.lastname: Zheng
authors.forename: Shuangyan
authors.initials: S
authors.lastname: Han
mesh_terms: D000272:Adipates / Q000378:metabolism*; D007218:Industrial Microbiology; D008049:Lipase / Q000378:metabolism*; D010843:Pichia / Q000378:metabolism*
chemical_list: D000272:Adipates; D008049:Lipase