title: Big angiotensin-25: a novel glycosylated angiotensin-related peptide isolated from human urine.
abstract: The renin-angiotensin system (RAS), including angiotensin II (Ang II), plays an important role in the regulation of blood pressure and body fluid balance. Consequently, the RAS has emerged as a key target for treatment of kidney and cardiovascular disease. In a search for bioactive peptides using an antibody against the N-terminal portion of Ang II, we identified and characterized a novel angiotensin-related peptide from human urine as a major molecular form. We named the peptide Big angiotensin-25 (Bang-25) because it consists of 25 amino acids with a glycosyl chain and added cysteine. Bang-25 is rapidly cleaved by chymase to Ang II, but is resistant to cleavage by renin. The peptide is abundant in human urine and is present in a wide range of organs and tissues. In particular, immunostaining of Bang-25 in the kidney is specifically localized to podocytes. Although the physiological function of Bang-25 remains uncertain, our findings suggest it is processed from angiotensinogen and may represent an alternative, renin-independent path for Ang II synthesis in tissue.
authors.affiliation: Circulatory and Body Fluid Regulation, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Nagata
authors.forename: Kinta
authors.initials: K
authors.lastname: Hatakeyama
authors.forename: Maki
authors.initials: M
authors.lastname: Asami
authors.forename: Mariko
authors.initials: M
authors.lastname: Tokashiki
authors.forename: Hajime
authors.initials: H
authors.lastname: Hibino
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nishiuchi
authors.forename: Kenji
authors.initials: K
authors.lastname: Kuwasako
authors.forename: Johji
authors.initials: J
authors.lastname: Kato
authors.forename: Yujiro
authors.initials: Y
authors.lastname: Asada
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kitamura
mesh_terms: D000595:Amino Acid Sequence; D000804:Angiotensin II / Q000378:metabolism* / Q000652:urine*; D006031:Glycosylation; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism / Q000652:urine; D014018:Tissue Distribution
chemical_list: D010455:Peptides; C587413:big angiotensin-25, human; D000804:Angiotensin II
keywords: Angiotensinogen; Bigangiotensin-25; Glycosyl chain; Renin–angiotensin system