title: Optimized antimicrobial peptide (Bacitracin) production by immobilized and free cells and of Bacillus Spp GU215 using Wood chips and silicon polymer beads.
abstract: The immobilization of bacillus spp. GU215 on silicon polymer beads, wood chips was performed and antibiotic peptide (bacitracin) production, optimization of parameters were investigated. The immobilized cells presented elevated levels of activity than free cells. The silicon polymer based cells showed widest zones of inhibitions (18mm) in 72 hours and 4% concentration of glucose, PH 8 and 30°C, whereas a marginal decrease in the activity (14mm) was noticed in case of wood chips based immobilization systems and least stable immobilization in 72 hours incubation time, 4% glucose concentration, PH 8 and 30°C. This study illustrates that the silicon polymer based beads facilitate a strong interactions with bacitracin producing cells and render them suitable for excessive and long time production of antibiotic.
authors.affiliation: Gomal Center of Biochemistry and Biotechnology (GCBB), Gomal University, D.I. Khan, KPK, Pakistan.
authors.forename: Adnan
authors.initials: A
authors.lastname: Amin
authors.forename: Muhammad Ayaz
authors.initials: MA
authors.lastname: Khan
authors.forename: Taufeeq
authors.initials: T
authors.lastname: Ahmad
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001407:Bacillus / Q000378:metabolism*; D001414:Bacitracin / Q000096:biosynthesis*; D018914:Cells, Immobilized; D006863:Hydrogen-Ion Concentration; D011108:Polymers; D014934:Wood
chemical_list: D000900:Anti-Bacterial Agents; D011108:Polymers; D001414:Bacitracin