title: Evaluation of vaccine candidate potential of deltaaroA, deltahtrA and deltaaroAdeltahtrA mutants of Salmonella enterica subspecies enterica serovar Abortusequi in guinea pigs.
abstract: Salmonella enterica subspecies enterica serovar Abortusequi (S. Abortusequi), a host adapted Salmonella causes abortions, still births and foal mortality in equids. Though known since more than 100 years, it is still a problem in many of the developing countries including India. There is dearth of really good vaccine affording immunity lasting at least for one full gestation. In search of a potential vaccine candidate, three defined deletion mutants (deltaaroA, deltahtrA and deltaaroAdeltahtrA) of S. Abortusequi were tested in guinea pig model for attenuation, safety, immunogenicity, humoral immune response, protective efficacy and persistence in host. The deltahtrA and deltaaroAdeltahtrA mutants were found to be safe on oral inoculation in doses as high as 4.2 x 10(9) cfu/animal. Also through subcutaneous inoculation deltaaroAdeltahtrA mutant did not induce any abortion in pregnant guinea pigs. All the three mutants did not induce any illness or death in 1-2 week-old baby guinea pigs except deltahtrA mutant which caused mortality on intraperitoneal inoculation. Inoculation with mutants protected against challenge and increased breeding efficiency of guinea pigs. After >4.5 months of mutant inoculation, guinea pigs were protected against abortifacient dose of wild type S. Abortusequi and mother guinea pigs also conferred resistance to their babies to the similar challenge. Early humoral immune response of S. Abortusequi mutants was characteristic. Faecal excretion of deltaaroA and htrA mutants was detected up to 45 days of inoculation in guinea pigs while deltaaroAdeltahtrA mutant could not be detected after 21 days of inoculation. The results indicated that the double deletion mutant (deltaaroAdeltahtrA) was the most effective and safe candidate for vaccination against S. Abortusequi through mucosal route of inoculation.
authors.affiliation: National Salmonella Centre (Vet), Centre for Animal Disease Research and Diagnosis, Indian Veterinary Research Institute (IVRI), Izatnagar 243 122, India. brs1762@yahoo.co.in
authors.forename: Bhoj Raj
authors.initials: BR
authors.lastname: Singh
authors.forename: Mudit
authors.initials: M
authors.lastname: Chandra
authors.forename: Dhananjoy
authors.initials: D
authors.lastname: Hansda
authors.forename: Javed
authors.initials: J
authors.lastname: Alam
authors.forename: Narayanan
authors.initials: N
authors.lastname: Babu
authors.forename: Mehtab Z
authors.initials: MZ
authors.lastname: Siddiqui
authors.forename: Ravi K
authors.initials: RK
authors.lastname: Agrawal
authors.forename: Gautam
authors.initials: G
authors.lastname: Sharma
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000917:Antibody Formation; D005260:Female; D017353:Gene Deletion; D006168:Guinea Pigs; D007194:India; D008297:Male; D009154:Mutation; D011247:Pregnancy; D011270:Pregnancy, Animal; D012481:Salmonella Infections, Animal / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D022562:Salmonella Vaccines / Q000235:genetics / Q000276:immunology*; D019779:Salmonella enterica / Q000235:genetics*; D013997:Time Factors; D014611:Vaccination
chemical_list: D022562:Salmonella Vaccines