title: Storage temperature and UV-irradiation influence on the ergosterol content in edible mushrooms.
abstract: Ergosterol (5,7,22-ergostatrien-3β-ol) and ergosteryl derivatives from different genera of edible mushrooms were separated and quantified by an isocratic reversed-phase high performance liquid chromatography (HPLC) method. The technique allowed a rapid separation of free ergosterol and two ergosteryl derivatives occurring in mushrooms. The ergosterol content varied considerably depending on the fungus. Thus, the species Agaricus bisporus and Hygrophorus marzuolus presented high quantities of ergosterol (6.4-6.8 mg/g, dry matter) followed by Pleurotus ostreatus, Calocybe gambosa, Lentinus edodes, and Boletus edulis (3.3-4.0mg/g). In contrast, other species, such as Cantharellus cibarius, Lactarius deliciosus and Craterellus cornucopioides, contained significantly lower ergosterol amounts (0.2-0.4 mg/g). Two ergosteryl derivatives were found in mushrooms and also the content depended on the fungus. The stability of ergosterol, in terms of the formation of ergosterol peroxide, was evaluated under different storage temperatures and UV radiation. The lower the temperature (-20°C) and the radiation time (10 min), the lower ergosterol oxidation was observed.
authors.affiliation: Centro para la Calidad de los Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus Universitario Duques de Soria, c/José Tudela s/n, 42004 Soria, Spain. Electronic address: anavillares@gmail.com.
authors.forename: Ana
authors.initials: A
authors.lastname: Villares
authors.forename: Laura
authors.initials: L
authors.lastname: Mateo-Vivaracho
authors.forename: Ana
authors.initials: A
authors.lastname: García-Lafuente
authors.forename: Eva
authors.initials: E
authors.lastname: Guillamón
mesh_terms: D000363:Agaricales / Q000737:chemistry* / Q000528:radiation effects*; D004875:Ergosterol / Q000032:analysis*; D061353:Food Storage / Q000379:methods*; D013696:Temperature; D014466:Ultraviolet Rays; D014675:Vegetables / Q000737:chemistry* / Q000528:radiation effects
chemical_list: D004875:Ergosterol
keywords: Ergosterol; Ergosteryl ester; Fungi; HPLC; Mushroom