title: Promotion of plantlet formation from somatic embryos of carrot treated with a high molecular weight extract from a marine cyanobacterium.
abstract: Somatic embryos of Daucus carota L. developed into plantlets at high frequency after addition of an extract from a marine cyanobacterium, Synechococcus sp. NKBG 042902. High molecular weight, nondialyzing fraction, separated from the extract, possessed enhanced plantlet formation promoting activity. Plantlet formation frequency was 60 % after addition of nondialysate (100 mg/l) compared to 28 % without addition. Embryos treated with the nondialysate contained five times more chlorophyll than nontreated embryos after 6 days of culture. The chlorophyll a/b ratio of 4-day old treated somatic embryos was found to be similar to that of zygotic embryos. However, the chlorophyll a/b ratio of plantlets induced from nontreated somatic embryos was variable. Nondialysate was fractionated by ultracentrifugation and an active component obtained, which gave a maximum plantlet formation frequency of 71 %, and induced rapid greening of shoots.
authors.affiliation: Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, 184, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Wake
authors.forename: A
authors.initials: A
authors.lastname: Akasaka
authors.forename: H
authors.initials: H
authors.lastname: Umetsu
authors.forename: Y
authors.initials: Y
authors.lastname: Ozeki
authors.forename: K
authors.initials: K
authors.lastname: Shimomura
authors.forename: T
authors.initials: T
authors.lastname: Matsunaga