title: Impact of CpG oligodeoxynucleotide stimulation on percentage of T and B cells in chicken.
abstract: TLR stimulation in chickens has been shown to play a role in the initiation and regulation of innate and adaptive immune responses. The aim of this study was to use flow cytometry to establish the percentage of T and B subset in blood and lymphoid organs in chicks after CpG oligodeoxynucleotide (ODN) stimulation. It was demonstrated that the percentages of CD3+, CD4+, TCRgamma delta+ cells and Bu-1+MHC class II+ cells in blood 24 h post-injection were significantly higher than in the control groups. It was also shown that the percentages of CD3+ and CD4+ cells in the spleen at 48 h post-injection were significantly higher than in control groups. The percentage of Bu-1+ cells in the bursa of Fabricius after CpG ODN stimulation (98.38 +/- 0.84) was significantly higher than that found in the non-CpG ODN control group (94.54 +/- 2.51) (p < or = 0.05). The results indicate that class B CpG ODN increases the percentage of both T (especially CD4+ cells) and B cells.
authors.affiliation: Department of Epizootiology and Clinic of Bird and Exotic Animals, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, pl. Grunwadzki 45, 50-366 Wrocław, Poland. klaudia.chrzastek@up.wroc.pl
authors.forename: K
authors.initials: K
authors.lastname: Chrzastek
authors.forename: T
authors.initials: T
authors.lastname: Piasecki
authors.forename: A
authors.initials: A
authors.lastname: Wieliczko
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D002645:Chickens; D008297:Male; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000502:physiology
chemical_list: D000276:Adjuvants, Immunologic; D009838:Oligodeoxyribonucleotides