title: Cloning and expression of soluble vascular endothelial growth factors receptor-1 (sFlt-1) fragments in CHO-K1.
abstract: Soluble form of vascular endothelial growth factor (VEGF) receptor (sFlt-1) has been considered a key target in anti angiogenesis strategies. In this study, sFlt-1 was amplified and cloned. For recombinant production of sFlt-1 protein, Chinese hamster ovary cell line (CHO) was used. The liposome-mediated transfection with sFlt-1 gene, were detected in sFlt-1 positive cells as early as 24 hours post-transfection. The production of sFlt-1 protein was confirmed using SDS-PAGE and immune blotting results. In present investigation, the recombinant protein of sFlt-1 had expressed with correct folding. The system is economically applicable for large production of sFlt protein and can be used as further therapeutic approaches in targeting the growth of solid tumor tissue.
authors.affiliation: Department of Biotechnology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences Tehran, Iran ; Experimental Medicine and Tissue Engineering Center, National Research Institute of Tuberculosis and Lung Disease (NRITLD) Tehran, Iran.
authors.forename: Poopak
authors.initials: P
authors.lastname: Farnia
authors.forename: Mojgan
authors.initials: M
authors.lastname: Bandehpour
authors.forename: Jalaledin
authors.initials: J
authors.lastname: Ghanavi
authors.forename: Bahram
authors.initials: B
authors.lastname: Kazemi
keywords: CHO cell lines; Soluble vascular endothelial growth factors receptor; angiogenesis