title: Nucleation of hydroxyapatite on Antheraea pernyi (A. pernyi) silk fibroin film.
abstract: Antheraea pernyi (A. pernyi) silk fibroin, which is spun from a wild silkworm, has increasingly attracted interest in the field of tissue engineering. The aim of this study was to investigate the nucleation of hydroxyapatite (HAp) on A. pernyi fibroin film. Von Kossa staining proved that A. pernyi fibroin had Ca binding activity. The A. pernyi fibroin film was mineralized with HAp crystals by alternative soaking in calcium and phosphate solutions. Spherical crystals were nucleated on the A. pernyi fibroin film according to scanning electron microscopeimaging results. The FT-IR and X-ray diffraction spectra confirmed that these spherical crystals were HAp. The results of in vitro cell culture using MG-63 cells demonstrated that the mineralized A. pernyi fibroin film showed excellent cytocompatibility and sound improvement of the MG-63 cellviability.
authors.affiliation: Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, 310058, China.
authors.forename: Mingying
authors.initials: M
authors.lastname: Yang
authors.forename: Yajun
authors.initials: Y
authors.lastname: Shuai
authors.forename: Guanshan
authors.initials: G
authors.lastname: Zhou
authors.forename: Namita
authors.initials: N
authors.lastname: Mandal
authors.forename: Liangjun
authors.initials: L
authors.lastname: Zhu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001861:Bone Regeneration; D001842:Bone and Bones / Q000378:metabolism; D002118:Calcium / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D003460:Crystallization; D017886:Durapatite / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry*; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D009036:Moths / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D014961:X-Ray Diffraction
chemical_list: D002130:Calcium Phosphates; D005349:Fibroins; D017886:Durapatite; C020243:calcium phosphate; D000469:Alkaline Phosphatase; D002118:Calcium
keywords: Antheraea pernyi fibroin; cell culture; hydroxyapatite; mineralization