title: Bioavailability of encapsulated resveratrol into nanoemulsion-based delivery systems.
abstract: Different O/W nanoemulsion-based delivery systems were developed in order to optimize the bioavailability of encapsulated resveratrol for potential oral administration. Blank formulations without resveratrol had no negative effect on cell viability or the cytoskeleton structure of Caco-2 cells (XTT viability assay and confocal microscopy). All nanoemulsions were then evaluated based on permeability tests on Caco-2 cells. As a result, the most efficient formulations were lecithin-based nanoemulsions which were able to transport resveratrol through cell monolayers in characteristically shorter times (1-6h) than those required for their metabolization (3-12h), allowing for better preservation of the integrity of the emulsion droplets, thus better protecting the resveratrol molecule. Fluorescence spectroscopy studies confirmed that resveratrol was encapsulated in the inner core of the nanoemulsions, which provides protection against chemical degradation. Furthermore, the developed systems also demonstrated the capability of nanoemulsions in sustained release of resveratrol from dialysis bags compared to the unencapsulated compound.
authors.affiliation: Department of Industrial Engineering, University of Salerno, Italy.
authors.forename: Mariarenata
authors.initials: M
authors.lastname: Sessa
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Balestrieri
authors.forename: Giovanna
authors.initials: G
authors.lastname: Ferrari
authors.forename: Luigi
authors.initials: L
authors.lastname: Servillo
authors.forename: Domenico
authors.initials: D
authors.lastname: Castaldo
authors.forename: Nunzia
authors.initials: N
authors.lastname: D'Onofrio
authors.forename: Francesco
authors.initials: F
authors.lastname: Donsì
authors.forename: Rong
authors.initials: R
authors.lastname: Tsao
mesh_terms: D001682:Biological Availability; D018938:Caco-2 Cells; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D004655:Emulsions / Q000737:chemistry; D006801:Humans; D010316:Particle Size; D010539:Permeability; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry* / Q000493:pharmacokinetics*
chemical_list: D004337:Drug Carriers; D004655:Emulsions; D013267:Stilbenes; D000077185:Resveratrol
keywords: Bioavailability; Cytotoxicity; In vitro release; O/W nanoemulsions; Resveratrol