title: Prohibitin is associated with antioxidative protection in hypoxia/reoxygenation-induced renal tubular epithelial cell injury.
abstract: Prohibitin is an evolutionary conserved and pleiotropic protein that has been implicated in various cellular functions, including proliferation, tumour suppression, apoptosis, transcription, and mitochondrial protein folding. We recently demonstrated that prohibitin downregulation results in increased renal interstitial fibrosis. Here we investigated the role of oxidative stress and prohibitin expression in a hypoxia/reoxygenation injury system in renal tubular epithelial cells with lentivirus-based delivery vectors to knockdown or overexpress prohibitin. Our results show that increased prohibitin expression was negatively correlated with reactive oxygen species, malon dialdehyde, transforming-growth-factor-β1, collagen-IV, fibronectin, and apoptosis (r = -0.895, -0.764, -0.798, -0.826, -0.817, -0.735; each P < 0.01), but positively correlated with superoxide dismutase, glutathione and mitochondrial membrane potential (r = 0.807, 0.815, 0.739; each P < 0.01). We postulate that prohibitin acts as a positive regulator of mechanisms that counteract oxidative stress and extracellular matrix accumulation and therefore has an antioxidative effect.
authors.affiliation: 1] Department of Pediatric Nephrology, the First Affiliated Hospital of GuangXi Medical University, NanNing 530021, China [2] Department of Nephrology, the Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China [3].
authors.forename: Tian-Biao
authors.initials: TB
authors.lastname: Zhou
authors.forename: Yuan-Han
authors.initials: YH
authors.lastname: Qin
authors.forename: Feng-Ying
authors.initials: FY
authors.lastname: Lei
authors.forename: Wei-Fang
authors.initials: WF
authors.lastname: Huang
authors.forename: Gregor P C
authors.initials: GP
authors.lastname: Drummen
mesh_terms: D058186:Acute Kidney Injury / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D005109:Extracellular Matrix / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism*; D007684:Kidney Tubules / Q000166:cytology*; D053078:Membrane Potential, Mitochondrial; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D000091402:Prohibitins; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D015427:Reperfusion Injury / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012097:Repressor Proteins / Q000378:metabolism*
chemical_list: D000091402:Prohibitins; D012333:RNA, Messenger; D012097:Repressor Proteins