title: Comparative analysis of putative orthologues of mitochondrial import motor subunit: Pam18 and Pam16 in plants.
abstract: Pam18/Tim14 and Pam16/Tim16, highly conserved proteins among eukaryotes, are two essential subunits of protein import motors localized in the inner mitochondrial membrane. The heterodimer formed by Pam18 and Pam16 via their J-type domains serves a regulatory function in protein translocation. Here, we report that thirty-one Pam18 and twenty-six Pam16 putative orthologues in twelve plant species were identified and analyzed through bioinformatics strategy. Results data revealed that Pam18 and Pam16 were also highly conserved among plants including their J-type domains within the hydrophilic region. Key amino acid residues and an HPD motif of Pam18 were identical among the orthologues except OsPam18L5. N-myristoylation sites of Pam18 and casein kinase II phosphorylation sites of Pam 16 were more abundant, which might be important functional sites. Some Pam18 and Pam16 proteins contained a transmembrane region at the N-terminal region. Sub-cellular prediction results indicated that many orthologues localized at mitochondria. Gene expression analyses revealed that Pam18 and Pam16 in Arabidopsis might play roles in senescence and abiotic stress responses. Our detailed study provides a better understanding of Pam18 and Pam16 in plant kingdom.
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Xuejin
authors.initials: X
authors.lastname: Chen
authors.forename: Bushra
authors.initials: B
authors.lastname: Ghazanfar
authors.forename: Abdul Rehman
authors.initials: AR
authors.lastname: Khan
authors.forename: Sikandar
authors.initials: S
authors.lastname: Hayat
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Cheng
mesh_terms: D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000235:genetics*; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods; D017124:Conserved Sequence / Q000235:genetics; D057225:Data Mining; D046228:Microarray Analysis; D033681:Mitochondrial Membrane Transport Proteins / Q000235:genetics*; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D010944:Plants / Q000235:genetics*; D016415:Sequence Alignment; D017385:Sequence Homology; D013045:Species Specificity
chemical_list: D029681:Arabidopsis Proteins; D033681:Mitochondrial Membrane Transport Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; C000588830:PAM16 protein, Arabidopsis thaliana