title: Hypoxia-inducible factor-1α (HIF-1α) protein diminishes sodium glucose transport 1 (SGLT1) and SGLT2 protein expression in renal epithelial tubular cells (LLC-PK1) under hypoxia.
abstract: In this work, we demonstrated the regulation of glucose transporters by hypoxia inducible factor-1α (HIF-1α) activation in renal epithelial cells. LLC-PK1 monolayers were incubated for 1, 3, 6, or 12 h with 0% or 5% O2 or 300 μm cobalt (CoCl2). We evaluated the effects of hypoxia on the mRNA and protein expression of HIF-1α and of the glucose transporters SGLT1, SGLT2, and GLUT1. The data showed an increase in HIF-1α mRNA and protein expression under the three evaluated conditions (p < 0.05 versus t = 0). An increase in GLUT1 mRNA (12 h) and protein expression (at 3, 6, and 12 h) was observed (p < 0.05 versus t = 0). SGLT1 and SGLT2 mRNA and protein expression decreased under the three evaluated conditions (p < 0.05 versus t = 0). In conclusion, our results suggest a clear decrease in the expression of the glucose transporters SGLT1 and SGLT2 under hypoxic conditions which implies a possible correlation with increased expression of HIF-1α.
authors.affiliation: From the Department of Pharmacology, School of Medicine, University of San Luis Potosi, 78210 San Luis Potosi, Mexico and.
authors.forename: Juan R
authors.initials: JR
authors.lastname: Zapata-Morales
authors.forename: Othir G
authors.initials: OG
authors.lastname: Galicia-Cruz
authors.forename: Martha
authors.initials: M
authors.lastname: Franco
authors.forename: Flavio
authors.initials: F
authors.lastname: Martinez Y Morales
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology; D015687:Cell Hypoxia / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003035:Cobalt / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D007684:Kidney Tubules / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051269:Sodium-Glucose Transporter 1 / Q000096:biosynthesis* / Q000235:genetics; D051297:Sodium-Glucose Transporter 2 / Q000096:biosynthesis* / Q000235:genetics; D013552:Swine; D013997:Time Factors
chemical_list: D016587:Antimutagenic Agents; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D051269:Sodium-Glucose Transporter 1; D051297:Sodium-Glucose Transporter 2; D003035:Cobalt; C018021:cobaltous chloride
keywords: GLUT1; Glucose Transport; Hypoxia; Hypoxia-inducible factor (HIF); Kidney Metabolism; Renal Glucose Transporters; Renal Physiology; SGLT1; SGLT2