title: Plant regeneration via somatic embryogenesis in chickpea (Cicer arietinum L.).
abstract: Efficient plant regeneration via somatic embryogenesis has been developed in chickpea cultivar C235. Leaf explants, on MS medium supplemented with 1.25 mg/l 2,4-D and 0.25 mg/l kinetin, yielded somatic embryos with high efficiency during dark incubation. MS medium supplemented with B5 vitamins, 0.125 mg/l IBA and 2 mg/l BAP was found suitable for embryo maturation. The well formed embryos germinated into plantlets on basal B5 medium supplemented with 0.25 mg/l BAP. Further development into healthy plantlets was obtained on basal B5 medium. Hardened plantlets produced normal, fertile plants upon transfer to soil.
authors.affiliation: Biotechnology Centre, Indian Agricultural Research Institute, 110 012, New Delhi, India.
authors.forename: V D
authors.initials: VD
authors.lastname: Kumar
authors.forename: P B
authors.initials: PB
authors.lastname: Kirti
authors.forename: J K
authors.initials: JK
authors.lastname: Sachan
authors.forename: V L
authors.initials: VL
authors.lastname: Chopra