title: Stable transformation of barley tissue culture by particle bombardment.
abstract: Suspension culture cells of barley (Hordeum vulgare L. cv Pokko) were stably transformed with two separate plasmids containing genes coding for neomycin phosphotransferase II and β-glucuronidase, respectively. Transformed cultures were selected with the antibiotic Geneticin(R). Enzymatic activity was tested in the Geneticin(R) resistant cultures, and in 96% of them neomycin phosphotransferase could be detected. The non-selected marker, detected as β-glucuronidase activity, was expressed in 40% of the resistant cultures. Stable transformation was confirmed with Southern blot hybridization.
authors.affiliation: VTT, Biotechnical Laboratory, PL 202, SF-02151, Espoo, Finland.
authors.forename: A
authors.initials: A
authors.lastname: Ritala
authors.forename: L
authors.initials: L
authors.lastname: Mannonen
authors.forename: K
authors.initials: K
authors.lastname: Aspegren
authors.forename: M
authors.initials: M
authors.lastname: Salmenkallio-Marttila
authors.forename: U
authors.initials: U
authors.lastname: Kurtén
authors.forename: R
authors.initials: R
authors.lastname: Hannus
authors.forename: J
authors.initials: J
authors.lastname: Mendez Lozano
authors.forename: T H
authors.initials: TH
authors.lastname: Teeri
authors.forename: V
authors.initials: V
authors.lastname: Kauppinen