title: Taurine attenuates lung ischemia-reperfusion injury after lung transplantation in rats.
abstract: OBJECTIVE
  Taurine, the major intracellular free amino acid found in high concentrations in mammalian cells, is known to be an endogenous antioxidant and a membrane-stabilizing agent. It was hypothesized that taurine may be effective in reducing ischemia-reperfusion injury after lung transplantation and an experimental study was conducted in a rat model.
  
  
  METHODS
  The number of Sprague-Dawley rats used in the study was 35. Animals were randomized into five groups of 7 rats each, including control, donor I, donor II, ischemia-reperfusion injury, and treatment groups. All animals were exposed to the same experimental conditions in the preoperative period. Rats were fixed in a supine position after the induction. After the rats were shaved, a left pneumonectomy was performed following sternotomy in control, donor I, and donor II groups. The harvested grafts in donor I and donor II groups were transplanted to the rats of the ischemia-reperfusion group and treatment group, respectively. However, taurine was administered intraperitoneally for 3 days before the harvesting procedure in donor II. All harvested lungs were kept in a Euro-Collins solution at +4 °C for 24 h in a half-inflated manner. After harvesting and transplantation, lungs were sampled for histopathological and biochemical analysis.
  
  
  RESULTS
  Malondialdehyde and superoxide dismutase, glutathione peroxidase, and catalase levels were lower in the treatment group than the other groups (p < 0.05). Histopathological findings were better in treatment group than the ischemia-reperfusion group (p < 0.05).
  
  
  CONCLUSIONS
  It was demonstrated that donor treatment with taurine resulted in preservation of transplanted lung tissue in respect to histopathological and biochemical findings.
authors.affiliation: Department of Anaesthesiology, Gazi University, Ankara, Turkey.
authors.forename: Leyla
authors.initials: L
authors.lastname: Guler
authors.forename: Murat
authors.initials: M
authors.lastname: Tavlasoglu
authors.forename: Orhan
authors.initials: O
authors.lastname: Yucel
authors.forename: Adem
authors.initials: A
authors.lastname: Guler
authors.forename: Mehmet Ali
authors.initials: MA
authors.lastname: Sahin
authors.forename: Mustafa
authors.initials: M
authors.lastname: Kurkluoglu
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Sirin
authors.forename: Ayse
authors.initials: A
authors.lastname: Eken
authors.forename: Mehmet
authors.initials: M
authors.lastname: Gamsizkan
authors.forename: Mehmet
authors.initials: M
authors.lastname: Dakak
authors.forename: Sedat
authors.initials: S
authors.lastname: Gurkok
authors.forename: Onur
authors.initials: O
authors.lastname: Genc
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D004195:Disease Models, Animal; D006982:Hypertonic Solutions; D008168:Lung / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D016040:Lung Transplantation / Q000379:methods; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D013482:Superoxide Dismutase / Q000032:analysis / Q000378:metabolism; D013654:Taurine / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D013654:Taurine; D008315:Malondialdehyde; D013482:Superoxide Dismutase