title: Biological activity of recombinant bovine interferon τ produced by a silkworm-baculovirus gene expression system.
abstract: Bovine interferon (bIFN) τ plays a crucial role in maternal-fetal recognition and was expressed using a Bombyx mori (Bm) nuclear polyhedrosis virus (silkworm baculovirus) gene expression system. The biological effects of Bm-recombinant bIFNτ (rbIFNτ) on prostaglandin (PG) F2α synthesis were investigated in cultured bovine endometrial epithelial cells with oxytocin (OT, 100 nM) and on the in vitro development of bovine embryos. Bm-rbIFNτ and OT were shown to suppress PGF2α production in a dose-dependent manner. When in vitro produced morula stage embryos were cultured for 72 hr in modified CR1aa medium supplemented with or without rbIFNτ, Bm-rbIFNτ (10 ng/ml) significantly promoted development to the expanded blastocyst stage. In conclusion, Bm-rbIFNτ was suggested to have the same bioactivity as native IFNτ.
authors.affiliation: Animal Breeding and Reproduction Research Division, NARO Institute of Livestock and Grassland Science, 2 Ikenodai, Tsukuba, Ibaraki 305-0901, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Takahashi
authors.forename: Makoto
authors.initials: M
authors.lastname: Tsunazaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Hamano
authors.forename: Masashi
authors.initials: M
authors.lastname: Takahashi
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Okuda
authors.forename: Shigeki
authors.initials: S
authors.lastname: Inumaru
authors.forename: Akira
authors.initials: A
authors.lastname: Okano
authors.forename: Masaya
authors.initials: M
authors.lastname: Geshi
authors.forename: Makoto
authors.initials: M
authors.lastname: Hirako
mesh_terms: D000818:Animals; D053898:Biosynthetic Pathways / Q000187:drug effects*; D012831:Bombyx / Q000378:metabolism* / Q000821:virology; D002417:Cattle; D015237:Dinoprost / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D007370:Interferon Type I / Q000096:biosynthesis* / Q000494:pharmacology*; D017924:Nucleopolyhedroviruses / Q000378:metabolism*; D010121:Oxytocin / Q000494:pharmacology; D011257:Pregnancy Proteins / Q000096:biosynthesis* / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology
chemical_list: D007370:Interferon Type I; D011257:Pregnancy Proteins; D011994:Recombinant Proteins; C020947:interferon tau; D010121:Oxytocin; D015237:Dinoprost