title: Effects of storage temperature on hematopoietic stability and microbial safety of BM aspirates.
abstract: Bone marrow (BM) remains a common source for hematopoietic SCT. Due to the transcutaneous approach, contamination with skin bacteria is common. The delay between harvest and transfusion can be considerable, potentially allowing for bacterial proliferation. The optimal transportation temperature, specifically with respect to bacterial growth and consequences thereof for hematopoietic quality, remain undefined. For 72 h, 66 individual BM samples, non-spiked/spiked with different bacteria, stored at 20-24 °C room temperature (RT) or 3-5 °C (cold), were serially analyzed for hematopoietic quality and microbial burden. Under most conditions, hematopoietic quality of BM was equal or better at RT: Typical BM contaminants (P. acnes and S. epidermidis) and E. coli were killed or bacterial proliferation was arrested at RT; hematopoietic quality was not impacted by the contamination. However, several pathogenic bacteria not typically found in BM (S. aureus and K. pneumoniae) proliferated dramatically at RT and impaired hematopoietic quality. Bacterial proliferation was arrested in the cold. The overwhelming majority of BM samples, that is, those that are sterile or contaminated only with skin commensals, will benefit from transportation at RT. Those bacteria that proliferate and perturb hematopoietic quality are not typically found in BM. Our data support recommendations for RT transportation and storage of BM.
authors.affiliation: German Red Cross Blood Service Baden-Wuerttemberg-Hessen and Institute for Transfusion Medicine and Immunohematology, Goethe University Medical Center, Frankfurt, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Hahn
authors.affiliation: German Red Cross Blood Service Baden-Wuerttemberg-Hessen and Institute for Transfusion Medicine and Immunohematology, Goethe University Medical Center, Frankfurt, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Sireis
authors.affiliation: German Red Cross Blood Service Baden-Wuerttemberg-Hessen and Institute for Transfusion Medicine and Immunohematology, Goethe University Medical Center, Frankfurt, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Hourfar
authors.affiliation: German Red Cross Blood Service Baden-Wuerttemberg-Hessen and Institute for Transfusion Medicine and Immunohematology, Goethe University Medical Center, Frankfurt, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Karpova
authors.affiliation: German Red Cross Blood Service Baden-Wuerttemberg-Hessen and Institute for Transfusion Medicine and Immunohematology, Goethe University Medical Center, Frankfurt, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Dauber
authors.affiliation: Institute for Medical Microbiology and Infection Control, Goethe University Medical Center, Frankfurt, Germany.
authors.forename: V A J
authors.initials: VA
authors.lastname: Kempf
authors.affiliation: German Red Cross Blood Service Baden-Wuerttemberg-Hessen and Institute for Transfusion Medicine and Immunohematology, Goethe University Medical Center, Frankfurt, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Seifried
authors.affiliation: German Red Cross Blood Service Baden-Wuerttemberg-Hessen and Institute for Transfusion Medicine and Immunohematology, Goethe University Medical Center, Frankfurt, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Schmidt
authors.affiliation: 1] German Red Cross Blood Service Baden-Wuerttemberg-Hessen and Institute for Transfusion Medicine and Immunohematology, Goethe University Medical Center, Frankfurt, Germany [2] Department of Medicine/Hematology, University of Washington, Seattle, WA, USA.
authors.forename: H
authors.initials: H
authors.lastname: Bönig
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001853:Bone Marrow / Q000382:microbiology / Q000473:pathology*; D004926:Escherichia coli; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007711:Klebsiella pneumoniae; D011425:Propionibacterium acnes; D012867:Skin / Q000382:microbiology; D013048:Specimen Handling / Q000379:methods*; D013211:Staphylococcus aureus; D013212:Staphylococcus epidermidis; D013234:Stem Cells; D013696:Temperature; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents