title: High-yield production of valepotriates by hairy root cultures of Valeriana officnalis L. var. sambucifolia Mikan.
abstract: Hairy root cultures of Valeriana officinalis var. sambucifolia were established by infection of sterile plantlets with Agrobacterium rhizogenes strain R1601 The transformed roots were grown in 10 different, hormone-free liquid media and the isovaltrate, valtrate, didrovaltrate, isovaleroxyhydroxydidrovaltrate content was quantified by HPLC. Valepotriates were entirely retained inside the root tissues. The highest overall valepotriate content (10.3 % dry wt), 4 times the amount found in the roots of 9-month-old nontransformed plants, was observed in half strength Gamborg B5 medium supplemented with 2 % sucrose. The hairy roots cultured in Murashige and Skoog liquid medium supplemented with 2 % sucrose for 50 days produced over 44 mg/g dry wt valepotriates.
authors.affiliation: Department of Pharmacognosy, University of Geneva, Sciences II, 30, Quai E.-Ansermet, CH-1211, 4, Geneva, Switzerland.
authors.forename: F
authors.initials: F
authors.lastname: Gränicher
authors.forename: P
authors.initials: P
authors.lastname: Christen
authors.forename: I
authors.initials: I
authors.lastname: Kapetanidis