title: A trial to determine D-amino acids in tissue proteins of mice.
abstract: Eleven neutral amino acids and two acidic amino acids in tissue proteins of mouse kidney, liver and brain were analyzed for the presence of D-enantiomers. The proteins were hydrolyzed with HCl for 6 h. Of the thirteen amino acids investigated, the presence of D-enantiomers of serine, alanine, proline, aspartate and glutamate (including asparagine and glutamine) was shown in the hydrolysates. However, the level of D-enantiomers were not significantly higher than that of 6-h hydrolysate of serum albumin examined as a control protein. Serum albumin was shown to contain no D-amino acid residues.
authors.affiliation: Department of Life Science, Himeji Institute of Technology, Shosha 2167, 671-22, Shosha, Himeji, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nagata
authors.forename: K
authors.initials: K
authors.lastname: Kubota