title: MiR-101 functions as a tumor suppressor by directly targeting nemo-like kinase in liver cancer.
abstract: Nemo-like kinase (NLK), an evolutionarily conserved MAP kinase-related kinase, has been reported to be involved in the development of hepatocellular carcinoma (HCC), but the underlying mechanisms leading to oncogenic NLK are poorly understood. A comprehensive microRNA (miRNA) profiling analysis on human HCC tissues identified four downregulated miRNAs that may target NLK. Ectopic expression of miRNA mimics suggested that miR-101 could suppress NLK in HCC cells. Notably, ectopic miR-101 expression repressed cancer cell growth and proliferation and imitated NLK knockdown effect on HCC cells. In conclusion, we suggest that miR-101 functions as a tumor suppressor by regulating abnormal NLK activity in liver.
authors.affiliation: Lab of Oncogenomics, Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Functional RNomics Research Center, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Shen
authors.affiliation: Lab of Oncogenomics, Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Functional RNomics Research Center, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Bae
authors.affiliation: Lab of Oncogenomics, Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Functional RNomics Research Center, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Jung Woo
authors.initials: JW
authors.lastname: Eun
authors.affiliation: Lab of Oncogenomics, Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Functional RNomics Research Center, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Hyung Seok
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Lab of Oncogenomics, Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Functional RNomics Research Center, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Se Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Lab of Oncogenomics, Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Functional RNomics Research Center, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Woo Chan
authors.initials: WC
authors.lastname: Shin
authors.affiliation: Cancer Evolution Research Center, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea.
authors.forename: Eun Kyung
authors.initials: EK
authors.lastname: Lee
authors.affiliation: Techno-Art Division, Underwood International College, Yonsei University, Seoul, Republic of Korea.
authors.forename: Soha
authors.initials: S
authors.lastname: Park
authors.affiliation: Lab of Oncogenomics, Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Functional RNomics Research Center, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Won Sang
authors.initials: WS
authors.lastname: Park
authors.affiliation: Lab of Oncogenomics, Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Functional RNomics Research Center, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Jung Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Lab of Oncogenomics, Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Functional RNomics Research Center, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Cancer Evolution Research Center, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. Electronic address: swnam@catholic.ac.kr.
authors.forename: Suk Woo
authors.initials: SW
authors.lastname: Nam
mesh_terms: D045744:Cell Line, Tumor; D015536:Down-Regulation; D055785:Gene Knockdown Techniques; D006801:Humans; D051550:I-kappa B Kinase / Q000037:antagonists & inhibitors* / Q000235:genetics* / Q000378:metabolism; D008113:Liver Neoplasms / Q000201:enzymology* / Q000235:genetics*; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection
chemical_list: C491731:IKBKG protein, human; C534072:MIRN101 microRNA, human; D035683:MicroRNAs; D051550:I-kappa B Kinase
keywords: Liver cancer; MiR-101; NLK; Tumor suppressor