title: The importance of Zebrafish in biomedical research.
abstract: BACKGROUND
  Zebrafish (Danio rerio) is an ideal model organism for the study of vertebrate development. This is due to the large clutches that each couple produces, with up to 200 embryos every 7 days, and to the fact that the embryos and larvae are small, transparent and undergo rapid external development.
  
  
  METHODS
  Using scientific literature research tools available online and the keywords Zebrafish, biomedical research, human disease, and drug screening, we reviewed original studies and reviews indexed in PubMed.
  
  
  RESULTS
  In this review we summarized work conducted with this model for the advancement of our knowledge related to several human diseases. We also focused on the biomedical research being performed in Portugal with the zebrafish model.
  
  
  CONCLUSIONS
  Powerful live imaging and genetic tools are currently available for zebrafish making it a valuable model in biomedical research. The combination of these properties with the optimization of automated systems for drug screening has transformed the zebrafish into "a top model" in biomedical research, drug discovery and toxicity testing. Furthermore, with the optimization of xenografts technology it will be possible to use zebrafish to aide in the choice of the best therapy for each patient.
  
  
  CONCLUSIONS
  Zebrafish is an excellent model organism in biomedical research, drug development and in clinical therapy.
authors.affiliation: Centro de Estudos de Doenças Crónicas. Faculdade de Ciências Médicas de Lisboa. Lisboa. Portugal. susana.lopes@fcm.unl.pt.
authors.forename: Bárbara
authors.initials: B
authors.lastname: Tavares
authors.forename: Susana
authors.initials: S
authors.lastname: Santos Lopes
mesh_terms: D000818:Animals; D035843:Biomedical Research / Q000379:methods*; D004195:Disease Models, Animal; D006801:Humans; D015027:Zebrafish