title: Toxicity inspired cross-linking for probing DNA-peptide interactions.
abstract: A cross-linking methodology for the study of DNA-protein interactions is described. The method is inspired by the metabolic activation of furans causing toxic DNA damage, including DNA-protein cross-links (DPC). The furan moiety, representing a latent functionality, is easily incorporated into oligonucleotides, and can be activated on demand to release a reactive aldehyde. Reaction with nucleophilic lysine side chains is shown to be distance-sensitive and allows for site-selective DPC formation.
authors.affiliation: Organic and Biomimetic Chemistry Research Group, Department of Organic Chemistry, Ghent University , Krijgslaan 281-S4, 9000 Gent, Belgium.
authors.forename: Lieselot L G
authors.initials: LL
authors.lastname: Carrette
authors.forename: Takashi
authors.initials: T
authors.lastname: Morii
authors.forename: Annemieke
authors.initials: A
authors.lastname: Madder
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004249:DNA Damage; D005663:Furans / Q000737:chemistry* / Q000633:toxicity; D008958:Models, Molecular; D015335:Molecular Probes / Q000737:chemistry* / Q000633:toxicity; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation
chemical_list: D003432:Cross-Linking Reagents; D005663:Furans; D015335:Molecular Probes; D010455:Peptides; D004247:DNA