title: Hematological, biochemical and enzymological responses in an Indian major carp Labeo rohita induced by sublethal concentration of waterborne selenite exposure.
abstract: Selenium (Se) pollution in aquatic ecosystem is an environmental issue throughout the world. Elevated concentrations of inorganic Se from agricultural and industrial processes may cause adverse biological effects in aquatic organisms such as fish. In the present study, Labeo rohita an Indian major carp were exposed to sublethal concentration of Se (sodium selenite) for 35days and certain hematological, biochemical and enzymological parameters were estimated. The median lethal concentration of waterborne sodium selenite (Na2SeO3) to L. rohita was found to be 23.89mgL(-1) for 96h. During sublethal (2.38mgL(-1)) treatment, hematological and biochemical parameters such as hemoglobin (Hb) (except 14th day), hematocrit (Hct), erythrocyte (RBC) count and protein levels were found to be decreased in Se treated fish whereas leucocyte (WBC) count and glucose level increased in Se treated fish throughout the study period. The enzymatic parameters such as glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT) and lactate dehydrogenase (LDH) activities were found to be increased in liver of Se treated fish L. rohita. A biphasic response was observed in the value of mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC). The alterations of these parameters can be used as suitable biomarkers in monitoring of selenium in the aquatic environment and to protect aquatic life.
authors.affiliation: Unit of Toxicology, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore 641046, Tamil Nadu, India.
authors.forename: Mathan
authors.initials: M
authors.lastname: Ramesh
authors.affiliation: Unit of Toxicology, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore 641046, Tamil Nadu, India.
authors.forename: Marimuthu
authors.initials: M
authors.lastname: Sankaran
authors.affiliation: Unit of Toxicology, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore 641046, Tamil Nadu, India.
authors.forename: Velusami
authors.initials: V
authors.lastname: Veera-Gowtham
authors.affiliation: Unit of Toxicology, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore 641046, Tamil Nadu, India. Electronic address: poopalramakrishanan@ymail.com.
authors.forename: Rama Krishnan
authors.initials: RK
authors.lastname: Poopal
mesh_terms: D000818:Animals; D024443:Aspartate Aminotransferase, Cytoplasmic / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism; D002347:Carps / Q000097:blood* / Q000378:metabolism*; D004781:Environmental Exposure; D004798:Enzymes / Q000378:metabolism*; D007194:India; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D018038:Sodium Selenite / Q000633:toxicity*; D000637:Transaminases; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D001786:Blood Glucose; D001798:Blood Proteins; D004798:Enzymes; D014874:Water Pollutants, Chemical; D007770:L-Lactate Dehydrogenase; D024443:Aspartate Aminotransferase, Cytoplasmic; D000637:Transaminases; D018038:Sodium Selenite
keywords: Biochemical; Enzymological parameters; Hematological; Labeo rohita; Selenium; Sublethal toxicity