title: Sargachromenol from Sargassum micracanthum inhibits the lipopolysaccharide-induced production of inflammatory mediators in RAW 264.7 macrophages.
abstract: During our ongoing screening program designed to determine the anti-inflammatory potential of natural compounds, we isolated sargachromenol from Sargassum micracanthum. In the present study, we investigated the anti-inflammatory effects of sargachromenol on lipopolysaccharide (LPS)-induced inflammation in murine RAW 264.7 macrophage cells and the underlying mechanisms. Sargachromenol significantly inhibited the LPS-induced production of nitric oxide (NO) and prostaglandin E₂ (PGE₂) in a dose-dependent manner. It also significantly inhibited the protein expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) in a dose-dependent manner in LPS-stimulated macrophage cells. Further analyses showed that sargachromenol decreased the cytoplasmic loss of inhibitor κBα (IκBα) protein. These results suggest that sargachromenol may exert its anti-inflammatory effects on LPS-stimulated macrophage cells by inhibiting the activation of the NF-κB signaling pathway. In conclusion, to our knowledge, this is the first study to show that sargachromenol isolated from S. micracanthum has an effective anti-inflammatory activity. Therefore, sargachromenol might be useful for cosmetic, food, or medical applications requiring anti-inflammatory properties.
authors.affiliation: Jeju Biodiversity Research Institute (JBRI), Jeju Technopark, Jeju 699-943, Republic of Korea.
authors.forename: Eun-Jin
authors.initials: EJ
authors.lastname: Yang
authors.forename: Young Min
authors.initials: YM
authors.lastname: Ham
authors.forename: Kyong-Wol
authors.initials: KW
authors.lastname: Yang
authors.forename: Nam Ho
authors.initials: NH
authors.lastname: Lee
authors.forename: Chang-Gu
authors.initials: CG
authors.lastname: Hyun
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D001578:Benzopyrans / Q000008:administration & dosage* / Q000378:metabolism; D002460:Cell Line; D015232:Dinoprostone / Q000276:immunology; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology* / Q000517:prevention & control*; D018836:Inflammation Mediators / Q000276:immunology*; D008070:Lipopolysaccharides; D008262:Macrophage Activation / Q000187:drug effects / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D009569:Nitric Oxide / Q000276:immunology; D044647:Sargassum / Q000378:metabolism*
chemical_list: D000893:Anti-Inflammatory Agents; D001578:Benzopyrans; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; C503204:sargachromenol; D009569:Nitric Oxide; D015232:Dinoprostone