title: [Analysis on microbial community in biofilm coating onto solid carbon source using the PCR-DGGE technique].
abstract: Polylactic acid/Poly (3-Hydroxybutyrate-co-3-Hydroxyvalerate) (PLA/PHBV) granules were used as both carbon source and biofilm carrier for nitrate removal from the contaminated water. The polymerase chain reaction-denature gradient gel electrophoresis (PCR-DGGE) was used to investigate the dynamic change of microbial community in the biofilm coating onto PLA/PHBV granules. The results showed that a decrease in microbial diversity was observed at the initial stage of reactor operation. Shannon-Wiener and Simpson diversity indexes of microbial community in the biofilm slightly changed during the stable period of the reactor running. The major microorganisms in the biofilm were Gram-negative rod bacteria including the genera of Diaphorobacter, Acidovorax, Rubrivivax, Azospira, Thermomonas and Devosia, and all of them belonged to alpha-, beta- and gamma- Proteobacteria, according to the SEM photo of biofilm and 16S rDNA sequences of the DGGE profile. Moreover, the abundance of Diaphorobacter-like genera was the highest in the solid-phase-denitrification biofilm.
authors.affiliation: Beijing Agro-Biotechnology Research Center, Beijing 100097, China. 1365437-7908@163.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.forename: Tian-Leii
authors.initials: TL
authors.lastname: Qiu
authors.forename: Mei-Lin
authors.initials: ML
authors.lastname: Han
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Xu-Ming
authors.initials: XM
authors.lastname: Wang
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000378:metabolism; D018441:Biofilms; D019149:Bioreactors; D002244:Carbon / Q000737:chemistry*; D004269:DNA, Bacterial / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D058440:Denitrification; D019344:Lactic Acid / Q000737:chemistry; D009566:Nitrates / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011108:Polymers / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D000094122:Polyhydroxybutyrates
chemical_list: D002244:Carbon; D004269:DNA, Bacterial; D019344:Lactic Acid; D009566:Nitrates; D011091:Polyesters; D011108:Polymers; D012336:RNA, Ribosomal, 16S; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C033616:poly(lactide)