title: Potential of sugarcane bagasse (agro-industrial waste) for the production of Bacillus thuringiensis israelensis.
abstract: Sugarcane bagasse is a renewable resource that can be used to produce biopesticide for the control of mosquito vectors. In the present study, we demonstrated that cane processed bagasse could be used to produce Bacillus thuringiensis serovar israelensis (Bti) for control of mosquito vectors viz: Culex quinquefasciatus, Anopheles stephensi and Aedes aegypti. Biochemical studies indicated that the Bti spore/crystal toxins produced from the test culture medium (Bagasse, BG + Soybean, SB) are higher than that from the conventional medium (Nutrient Yeast Extract Salt Medium, NYSM). The bacteria produced in these media (NYSM, BG, SB, BG+SB) were bioassayed against the mosquito species and the toxic effect was found to be effective. Cost-effective analysis indicates that the use of BG and SB, as bacterial culture medium, is successful and economical, for production of this mosquito pathogenic bacillus.
authors.affiliation: Unit of Microbiology and Immunology, Vector Control Research Centre (Indian Council of Medical Research), Medical complex, Indira Nagar, Pondicherry - 605006, India.
authors.forename: S
authors.initials: S
authors.lastname: Poopathi
authors.forename: C
authors.initials: C
authors.lastname: Mani
authors.forename: G
authors.initials: G
authors.lastname: Rajeswari
mesh_terms: D000330:Aedes / Q000382:microbiology / Q000502:physiology; D000818:Animals; D000852:Anopheles / Q000382:microbiology / Q000502:physiology; D001413:Bacillus thuringiensis / Q000254:growth & development* / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000378:metabolism*; D001681:Biological Assay / Q000379:methods; D002482:Cellulose / Q000378:metabolism*; D003465:Culex / Q000382:microbiology / Q000502:physiology; D003470:Culture Media / Q000737:chemistry*; D004731:Endotoxins / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods*; D007220:Industrial Waste; D007306:Insecticides / Q000378:metabolism / Q000494:pharmacology; D031786:Saccharum; D016019:Survival Analysis
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D003470:Culture Media; D004731:Endotoxins; D006460:Hemolysin Proteins; D007220:Industrial Waste; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D002482:Cellulose; C027433:bagasse