title: Cloning, purification, crystallization and preliminary X-ray studies of the putative transcriptional regulator SPD0280 from Streptococcus pneumoniae D39.
abstract: Streptococcus pneumoniae SPD0280 is a hypothetical protein that has been putatively identified as a transcriptional regulator. However, it has very low sequence identity to other well characterized transcriptional regulators. Determination of the three-dimensional structure may provide information for the characterization of proteins; therefore, it was decided to use X-ray diffraction analysis to learn more about this protein. Here, the expression, purification, crystallization and preliminary crystallographic analysis of SPD0280 from S. pneumoniae are reported. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 45.886, b = 66.785, c = 150.050 Å, and diffracted to a resolution of 2.5 Å. The crystals are likely to contain one molecule in the asymmetric unit, with a VM value of 2.06 Å(3) Da(-1).
authors.affiliation: Key Laboratory of Molecular Biology on Infectious Disease, Chongqing Medical University, YiXueYuanlu-1, Chongqing 400016, People's Republic of China.
authors.forename: Shaocheng
authors.initials: S
authors.lastname: Zhang
authors.forename: Xun
authors.initials: X
authors.lastname: Min
authors.forename: Zhen
authors.initials: Z
authors.lastname: Guo
authors.forename: Hongpeng
authors.initials: H
authors.lastname: Zhang
authors.forename: Ailong
authors.initials: A
authors.lastname: Huang
authors.forename: Yibing
authors.initials: Y
authors.lastname: Yin
authors.forename: Deqiang
authors.initials: D
authors.lastname: Wang
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D013296:Streptococcus pneumoniae / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D014157:Transcription Factors
keywords: SPD0280; Streptococcus pneumoniae