title: Improvement of L-arabinose fermentation by modifying the metabolic pathway and transport in Saccharomyces cerevisiae.
abstract: The L-arabinose utilization pathway was established in Saccharomyces cerevisiae, by expressing the codon-optimized araA, araB, and araD genes of Lactobacillus plantarum. After overexpressing the TAL1, TKL1, RPE1, RKI1, and GAL2 genes and adaptive evolution, the L-arabinose utilization of the recombinant strain became efficient. The resulting strain displayed a maximum specific growth rate of 0.075 h(-1), a maximum specific L-arabinose consumption rate of 0.61 g h(-1) g(-1) dry cell weight, and a promising ethanol yield of 0.43 g g(-1) from L-arabinose fermentation.
authors.affiliation: The State Key Laboratory of Microbial Technology, Shandong University, Shan Da Nan Road No. 27, Jinan 250100, China.
authors.forename: Chengqiang
authors.initials: C
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Shen
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Fan
authors.initials: F
authors.lastname: Suo
authors.forename: Jin
authors.initials: J
authors.lastname: Hou
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Bao
mesh_terms: D001089:Arabinose / Q000235:genetics* / Q000378:metabolism; D001692:Biological Transport; D000431:Ethanol / Q000138:chemical synthesis*; D005285:Fermentation; D005947:Glucose / Q000737:chemistry; D048191:Lactiplantibacillus plantarum / Q000235:genetics; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
chemical_list: D000431:Ethanol; D001089:Arabinose; D005947:Glucose