title: Effect of autologous adipose-derived stem cells in renal cold ischemia and reperfusion injury.
abstract: OBJECTIVE
  The aim of this study was to investigate the effect of autologous adipose-derived stem cells (ADSCs) on renal cold ischemia and reperfusion (I/R) injury via intravenous infusion on rats.
  
  
  METHODS
  A renal cold I/R injury rat model was established. Rats were equally randomized into Sham group, Cold I/R group (cold I/R plus culture medium only), and ADSC-treated group (cold I/R plus immediate intrarenal administration of 2 × 10(6) autologous ADSCs, followed by intravenous autologous ADSCs 6 hours after reperfusion). All rats were killed 24 hours after the I/R procedure.
  
  
  RESULTS
  Serum creatinine levels were significantly reduced in the ADSC-treated group compared with the Cold I/R group (P < .01). The renal tissue in the ADSC-treated group had well conserved renal architecture compared with the Cold I/R group. The mRNA expression of tumor necrosis factor α was significantly lower and Bcl-2 was higher in the ADSC-treated group than in the Cold I/R group (P < .05).
  
  
  CONCLUSIONS
  Autologous ADSC infusions ameliorated renal damage undergoing cold I/R injury and improved the renal function, partly through inhibiting inflammatory reactions and reducing apoptosis.
authors.affiliation: Department of Transplantation Surgery, Central Laboratory, Tianjin First Central Hospital, Key Lab for Critical Care Medicine of the Ministry of Health, Tianjin, China.
authors.forename: Y-L
authors.initials: YL
authors.lastname: Wang
authors.forename: G
authors.initials: G
authors.lastname: Li
authors.forename: X-F
authors.initials: XF
authors.lastname: Zou
authors.forename: X-B
authors.initials: XB
authors.lastname: Chen
authors.forename: T
authors.initials: T
authors.lastname: Liu
authors.forename: Z-Y
authors.initials: ZY
authors.lastname: Shen
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D001483:Base Sequence; D003080:Cold Temperature; D003404:Creatinine / Q000097:blood; D017931:DNA Primers; D007668:Kidney / Q000098:blood supply*; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D015427:Reperfusion Injury / Q000473:pathology*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D017931:DNA Primers; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D003404:Creatinine