title: In trans T cell tolerance exacerbates experimental allergic encephalomyelitis by interfering with protective antibody responses.
abstract: F1 (SJL/J×C57BL/6) mice with MOG35-55-induced EAE recover from disease when treated with Ig-MOG carrying MOG35-55 peptide. However, Ig-PLP1, carrying PLP139-151, induced reduction of anti-MOG antibodies and exacerbated EAE. Herein, we show that Ig-PLP1 specifically reduces the frequency of B cells producing protective IgG2a/b anti-MOG antibodies. Surprisingly, these cells were marginal zone (MZ), rather than follicular (FO) or newly formed (NF), B cells and transfer of MZ B cells into sick mice nullified disease exacerbation by Ig-PLP1 in a complement dependent manner. These findings reveal a potential self-limiting regulatory mechanism involving auto-antibodies in MOG EAE.
authors.affiliation: Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, M616 Medical Sciences Building, Columbia, MO 65212, USA.
authors.forename: Jason A
authors.initials: JA
authors.lastname: Cascio
authors.affiliation: Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, M616 Medical Sciences Building, Columbia, MO 65212, USA.
authors.forename: Marie-Therese
authors.initials: MT
authors.lastname: Khairallah
authors.affiliation: Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, M616 Medical Sciences Building, Columbia, MO 65212, USA.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Wan
authors.affiliation: Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, M616 Medical Sciences Building, Columbia, MO 65212, USA.
authors.forename: Weirong
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, M616 Medical Sciences Building, Columbia, MO 65212, USA.
authors.forename: Linda M
authors.initials: LM
authors.lastname: Rowland
authors.affiliation: Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, M616 Medical Sciences Building, Columbia, MO 65212, USA.
authors.forename: Mermagya
authors.initials: M
authors.lastname: Dhakal
authors.affiliation: Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, M616 Medical Sciences Building, Columbia, MO 65212, USA.
authors.forename: Mindy M
authors.initials: MM
authors.lastname: Miller
authors.affiliation: Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, M616 Medical Sciences Building, Columbia, MO 65212, USA; Department of Child Health, University of Missouri School of Medicine, M616 Medical Sciences Building, Columbia, MO 65212, USA; Department of Neurology, University of Missouri School of Medicine, M616 Medical Sciences Building, Columbia, MO 65212, USA. Electronic address: zaghouanih@health.missouri.edu.
authors.forename: Habib
authors.initials: H
authors.lastname: Zaghouani
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000276:immunology* / Q000628:therapy*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D007108:Immune Tolerance / Q000502:physiology*; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000009:adverse effects; D010446:Peptide Fragments / Q000009:adverse effects; D018709:Statistics, Nonparametric; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000906:Antibodies; D016207:Cytokines; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; C445081:myelin oligodendrocyte glycoprotein (35-55)
keywords: Anti-MOG antibodies; Autoimmunity; B cells; EAE; T cell tolerance