title: Absence of mutagenic and recombinagenic activity of multi-walled carbon nanotubes in the Drosophila wing-spot test and Allium cepa test.
abstract: In order to assess the safety of the carbon nanotubes to human health and the environment, we investigated the potential toxicity and ability of multi-walled carbon nanotubes (NT), to induce DNA damage by employing the Allium cepa genotoxicity/mutagenicity test and the Somatic Mutation and Recombination Test (SMART) in the fruitfly, Drosophila melanogaster. The results demonstrated that NT did not significantly induce genotoxic or mutagenic effects in the Allium cepa test. All concentrations evaluated in the SMART assay showed survival rates higher than 90percent, indicating the absence of chronic toxicity for NT. Furthermore, the various treatments showed no significant increase in the NT mutation and recombination frequencies in mwh/flr(3) genotype compared to respective negative controls, demonstrating the absence of DNA damage caused by NT.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Laise Rodrigues
authors.initials: LR
authors.lastname: de Andrade
authors.forename: Arian Sandin
authors.initials: AS
authors.lastname: Brito
authors.forename: Anna Maria Gouvea de Souza
authors.initials: AM
authors.lastname: Melero
authors.forename: Hudson
authors.initials: H
authors.lastname: Zanin
authors.forename: Helder José
authors.initials: HJ
authors.lastname: Ceragioli
authors.forename: Vitor
authors.initials: V
authors.lastname: Baranauskas
authors.forename: Kênya Silva
authors.initials: KS
authors.lastname: Cunha
authors.forename: Silvia Pierre
authors.initials: SP
authors.lastname: Irazusta
mesh_terms: D000818:Animals; D004249:DNA Damage / Q000187:drug effects; D004331:Drosophila melanogaster / Q000187:drug effects*; D009152:Mutagenicity Tests; D009154:Mutation / Q000187:drug effects; D037742:Nanotubes, Carbon / Q000737:chemistry / Q000633:toxicity* / Q000648:ultrastructure; D019697:Onions / Q000187:drug effects*; D011995:Recombination, Genetic / Q000187:drug effects; D014921:Wings, Animal / Q000187:drug effects
chemical_list: D037742:Nanotubes, Carbon
keywords: Genotoxicity; Mutation; Nanoparticles; Recombination