title: Solution NMR studies of the plant peptide hormone CEP inform function.
abstract: The C-terminally Encoded Peptide (CEP) family of regulatory peptides controls root development in vascular plants. Here, we present the first NMR structures of CEP. We show that root-knot nematode (RKN: Meloidogyne spp.) also encodes CEP, presumably to mimic plant CEP as part of their stereotypic, parasitic interaction with vascular plants. Molecular dynamics simulations of plant- and nematode-encoded CEP displaying known posttranslational modifications (PTM) provided insight into the structural effects of PTM and the conformational plasticity and rigidity of CEP. Potential mechanisms of action are discussed with respect to the structure and sampling of conformational space.
authors.affiliation: Department of Molecular and Structural Biochemistry, NC State University, Raleigh, NC 27695, United States.
authors.forename: Benjamin G
authors.initials: BG
authors.lastname: Bobay
authors.forename: Peter
authors.initials: P
authors.lastname: DiGennaro
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Scholl
authors.forename: Nijat
authors.initials: N
authors.lastname: Imin
authors.forename: Michael A
authors.initials: MA
authors.lastname: Djordjevic
authors.forename: David
authors.initials: D
authors.lastname: Mck Bird
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018745:Genome, Plant / Q000502:physiology; D006790:Host-Parasite Interactions / Q000502:physiology; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D018716:Molecular Mimicry / Q000502:physiology; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology; D010937:Plant Growth Regulators / Q000737:chemistry* / Q000502:physiology*; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D053204:Root Nodules, Plant / Q000378:metabolism / Q000469:parasitology; D017386:Sequence Homology, Amino Acid; D014431:Tylenchoidea / Q000235:genetics / Q000378:metabolism*
chemical_list: D010937:Plant Growth Regulators
keywords: CEP; CLE; Meloidogyne; Molecular dynamic; NMR; Peptide; Root-knot nematode