title: Catecholamine synthesis in the rainbow trout (Oncorhynchus mykiss) brain: modulation of tyrosine hydroxylase activity.
abstract: Levels of catecholamines and tyrosine hydroxylase (TH) activity were measured in brain homogenates from female rainbow trout. In triploid fish or in diploid fish in ovarian recrudescence, the patterns of catecholamine content expressed as a function of in vitro TH activity vary in different areas of the brain. Km for the pterin cofactor is lower in the telencephalon than in the hypothalamus. Dopamine (DA) and 2-hydroxyestradiol (20HE2) inhibit TH activity (by competitive and non-competitive interaction respectively).The K1 for DA were different in the telencephalon and the hypothalamus and this could explain the different patterns of catecholamine levels and TH activity for these two structures. 20HE2 inhibits TH activity in vitro; a catechol moiety is required since estradiol (E2) is notinhibitory. However, the exact mechanism of inhibition remains unclear. The rapid effect of 20HE2 cannot explain the previously reported activation of catecholamine synthesis by E2 in vivo.
authors.affiliation: Laboratoire de Physiologie des Régulations, URA CNRS 256 - Campus de Beaulieu, 35042, Rennes Cédex, France.
authors.forename: C
authors.initials: C
authors.lastname: Saligaut
authors.forename: S
authors.initials: S
authors.lastname: Bennani
authors.forename: T
authors.initials: T
authors.lastname: Bailhache