title: An enzyme containing microemulsion based on skin friendly oil and surfactant as decontamination medium for organo phosphates: phase behavior, structure, and enzyme activity.
abstract: The present contribution presents a microemulsion system containing cosmetic oil and sugar surfactant and the enzyme diisopropyl fluorophosphatase (DFPase) as active agent for the decontamination of human skin. The bicontinuous structure and the physical properties of the microemulsion are characterized by dynamic light scattering and small angle neutron scattering. The DFPase from the squid Loligo vulgaris is catalyzing the hydrolysis of highly toxic organophosphates. The effect of the enzyme on the structure of the microemulsion is investigated. Moreover, the enzyme/microemulsion system is also studied with respect to its activity using nuclear magnetic resonance spectroscopy leading to promising results. A fast decomposition of the nerve agent sarin is achieved.
authors.affiliation: Lehrstuhl Biomoleculare NMR-Spektroskopie, Department Chemie, Technische Universität München, Lichtenbergstrasse 4, D-85747 Garching, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Stehle
authors.forename: Christoph
authors.initials: C
authors.lastname: Schulreich
authors.forename: Stefan
authors.initials: S
authors.lastname: Wellert
authors.forename: Jürgen
authors.initials: J
authors.lastname: Gäb
authors.forename: Marc-Michael
authors.initials: MM
authors.lastname: Blum
authors.forename: Kai
authors.initials: K
authors.lastname: Kehe
authors.forename: Andras
authors.initials: A
authors.lastname: Richardt
authors.forename: Alain
authors.initials: A
authors.lastname: Lapp
authors.forename: Thomas
authors.initials: T
authors.lastname: Hellweg
mesh_terms: D004655:Emulsions; D015394:Molecular Structure; D009821:Oils / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry*; D012867:Skin; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D004655:Emulsions; D009821:Oils; D009943:Organophosphorus Compounds; D013501:Surface-Active Agents
keywords: DFPase; Decontamination; Dynamic light scattering; GB; Microemulsion; Protein; Small angle neutron scattering