title: Sensitization of transforming growth factor-β signaling by multiple peptides patterned on DNA nanostructures.
abstract: We report sensitization of a cellular signaling pathway by addition of functionalized DNA nanostructures. Signaling by transforming growth factor β (TGFβ) has been shown to be dependent on receptor clustering. By patterning a DNA nanostructure with closely spaced peptides that bind to TGFβ receptor, we observe increased sensitivity of NMuMG cells to TGFβ ligand. This is evidenced by translocation of secondary messenger proteins to the nucleus and stimulation of an inducible luciferase reporter at lower concentrations of TGFβ ligand. We believe this represents an important initial step toward realization of DNA as a self-assembling and biologically compatible material for use in tissue engineering and drug delivery.
authors.affiliation: Department of Chemistry, Duke University , 124 Science Drive, Durham, North Carolina 27708-0354, United States.
authors.forename: Ronnie O
authors.initials: RO
authors.lastname: Pedersen
authors.forename: Elizabeth G
authors.initials: EG
authors.lastname: Loboa
authors.forename: Thomas H
authors.initials: TH
authors.lastname: LaBean
mesh_terms: D000818:Animals; D001710:Biotin / Q000737:chemistry; D002460:Cell Line; D004247:DNA / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry / Q000494:pharmacology*; D017930:Genes, Reporter; D051379:Mice; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism; D015398:Signal Transduction; D019809:Streptavidin / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000494:pharmacology / Q000502:physiology*
chemical_list: D004337:Drug Carriers; D010446:Peptide Fragments; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D001710:Biotin; D004247:DNA; D019809:Streptavidin