title: A tetracycline-inducible expression vector for Streptococcus agalactiae allowing controllable gene expression.
abstract: Streptococcus agalactiae is the principal cause of neonatal infections; however, for its study, genetic tools are scarce. We have developed pG+off, an expression vector with a tetracycline inducible promoter that allows a tightly-controllable gene expression in S. agalactiae. Using a reporter gene, we have shown that pG+off is characterized by tight repression under non-induced condition.
authors.affiliation: Institut de Génétique et Microbiologie, CNRS/UMR 8621, Centre scientifique d'Orsay, Université Paris-Sud, Bâtiment 400, 91405 Orsay Cedex, France; CHRU de Tours, Université François Rabelais de Tours, UMR1282 ISP, F-37032 Tours, France; INRA, UMR1282 ISP, F-37380 Nouzilly, France. Electronic address: lartigue@med.univ-tours.fr.
authors.forename: Marie-Frédérique
authors.initials: MF
authors.lastname: Lartigue
authors.affiliation: Institut de Génétique et Microbiologie, CNRS/UMR 8621, Centre scientifique d'Orsay, Université Paris-Sud, Bâtiment 400, 91405 Orsay Cedex, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Bouloc
mesh_terms: D015870:Gene Expression / Q000187:drug effects*; D017930:Genes, Reporter; D005822:Genetic Vectors; D005827:Genetics, Microbial / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D013292:Streptococcus agalactiae / Q000235:genetics*; D013752:Tetracycline / Q000378:metabolism*; D015533:Transcriptional Activation / Q000187:drug effects*
chemical_list: D013752:Tetracycline
keywords: Controllable gene expression; Inducible expression vector; Streptococcus agalactiae