title: Crystallization and X-ray crystallographic analysis of the cholesterol-dependent cytolysin listeriolysin O from Listeria monocytogenes.
abstract: The secreted pore-forming toxin listeriolysin O (LLO) from the intracellular pathogen Listeria monocytogenes is a member of the family of cholesterol-dependent cytolysins (CDC) with broad properties in pathogenesis. Its role as a virulence factor is enigmatic: it disrupts membranes and acts as an inductor of both pro- and anti-inflammatory responses in infected cells. In addition, LLO is also a potent target for immunogenicity during infection. Natively secreted LLO from a recombinant L. innocua strain was crystallized in its water-soluble monomeric form. The crystals obtained belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 26.7, b = 85.1, c = 230.0 Å, and diffracted to beyond 2.2 Å resolution. The Matthews coefficient and the solvent content were estimated to be 2.4 Å(3) Da(-1) and 49.2%, respectively. The structure with one molecule in the asymmetric unit was solved using Phaser employing the structure of the previously characterized CDC toxin perfringolysin O as a search model.
authors.affiliation: Department of Structural Biology, Max Planck Institute of Biophysics, Max-von-Laue Strasse 3, 60438 Frankfurt am Main, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Köster
authors.forename: Martina
authors.initials: M
authors.lastname: Hudel
authors.forename: Trinad
authors.initials: T
authors.lastname: Chakraborty
authors.forename: Özkan
authors.initials: Ö
authors.lastname: Yildiz
mesh_terms: D001427:Bacterial Toxins / Q000737:chemistry* / Q000302:isolation & purification; D002784:Cholesterol / Q000378:metabolism*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000302:isolation & purification; D006460:Hemolysin Proteins / Q000737:chemistry* / Q000302:isolation & purification; D008089:Listeria monocytogenes / Q000378:metabolism*
chemical_list: D001427:Bacterial Toxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D002784:Cholesterol; C038705:hlyA protein, Listeria monocytogenes
keywords: Listeria monocytogenes; cholesterol-dependent cytolysins; listeriolysin O; listeriolysis; toxins