title: Residual treatment of Aedes aegypti (Diptera: Culicidae) in containers using pyriproxyfen slow-release granules (Sumilarv 0.5G).
abstract: The residual efficacy of pyriproxyfen against Aedes aegypti (L.) was examined by treating 2-liter buckets with a range of rates of Sumilarv 0.5G (100, 10, 1, and 0.1 mg product/liter or nominal dose of 500, 50, 5, and 0.5 ppb active ingredient) under semifield conditions. Approximately every 2 wk, pupal emergence inhibition (EI) was measured by using Cairns colony Ae. aegypti. Pooled water samples from the five replicate buckets were analyzed for active pyriproxyfen by using ultra-high-pressure liquid chromatography with tandem mass spectrometry detection. A strong dose-response in EI was exhibited, with the 0.1 mg/liter giving approximately 50% EI for only the initial week, whereas the 10 and 100 mg/liter doses produced EI > 90% for 8 and 40 wk, respectively. Measurable levels of active ingredient were detected in the 100, 10, and 1 mg/liter treatments, with measured starting concentrations of just 1-2-1.4% of the delivered (active ingredient) dose. Pyriproxyfen was detected in the 100 mg/liter treatment through the entire course of the trial (60 wk).
authors.affiliation: School of Public Health, Tropical Medicine and Rehabilitation Sciences, James Cook University, Cairns, Queensland, Australia. scott.ritchie@jcu.edu.au
authors.forename: Scott A
authors.initials: SA
authors.lastname: Ritchie
authors.forename: Chris
authors.initials: C
authors.lastname: Paton
authors.forename: Tamara
authors.initials: T
authors.lastname: Buhagiar
authors.forename: Garry A
authors.initials: GA
authors.lastname: Webb
authors.forename: Vladan
authors.initials: V
authors.lastname: Jovic
mesh_terms: D000330:Aedes; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007306:Insecticides; D007814:Larva; D009032:Mosquito Control; D011679:Pupa; D011725:Pyridines; D011793:Queensland; D053719:Tandem Mass Spectrometry; D014867:Water / Q000032:analysis
chemical_list: D007306:Insecticides; D011725:Pyridines; D014867:Water; C055613:pyriproxyfen