title: Regeneration of plants from callus tissue of the pasture legume Centrosema brasilianum.
abstract: Plants were regenerated from leaf explants of Centrosema brasilianum cultured in vitro. Callus and buds were produced on Murashige and Skoog medium (MS), 0.8% agar, 0.1 mg/l NAA and 1 mg/l BAP. Regeneration of multiple shoots was achieved by transferring callus onto fresh medium containing 0.01 and 1 mg/l of NAA and BAP, respectively. Shoots formed roots upon transfer to MS with 0.01 mg/l NAA. Plantlets were succesfully transferred to soil. Leaf-derived calli of Centrosema arenarium, C. macrocarpum, C. pascuorum, C. pubescens, and C. virginianum did not produce shoots when cultured in vitro.
authors.affiliation: Facultad de Ciencias Agrarias (UNNE), Instituto de Botánica del Nordeste, Casilla de Correos 209, 3400, Corrientes, Argentina.
authors.forename: P N
authors.initials: PN
authors.lastname: Angeloni
authors.forename: H Y
authors.initials: HY
authors.lastname: Rey
authors.forename: L A
authors.initials: LA
authors.lastname: Mroginski