title: Indole conjugated silica and magnetic nanoparticles as inhibitors of HIF.
abstract: Multifunctional silica nano-vehicles (SiO2@indol-IL) and magnetic nanoparticles (Fe3O4@indol-IL) were constructed through the Schiff bases condensation of indole-3-carboxaldehyde and 4-acetyl-N-allyl pyridinium chloride (ILs) with the amine groups of silica and magnetic nanoparticles. SiO2@indol-IL can inhibit the proliferation of HepG-2 cells in 48 h. Fe3O4@indol-IL can mimic the function of catalase to disproportionate H2O2 to O2, and has obvious effect on the proliferation of HepG-2 cells in 72 h. Moreover, the two nanoparticles show some inhibition on the expression of hypoxia inducible factor (HIF-1α), glucose transporter (GLUT1) and the production of lactate in HepG-2 cells. Therefore, we deduced that indole conjugated silica and magnetic nanparticles could be used as inhibitors of HIF-1α or GLUT1.
authors.affiliation: School of Chemistry and Chemical Engineer, Jiangsu University, Zhenjiang 212013, PR China; State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, PR China. Electronic address: chenqy@ujs.edu.cn.
authors.forename: Qiu-Yun
authors.initials: QY
authors.lastname: Chen
authors.affiliation: School of Chemistry and Chemical Engineer, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineer, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Yang
authors.affiliation: School of Chemistry and Chemical Engineer, Jiangsu University, Zhenjiang 212013, PR China; State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
mesh_terms: D002374:Catalase / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D051272:Glucose Transporter Type 1 / Q000235:genetics / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007211:Indoles / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D007700:Kinetics; D019344:Lactic Acid / Q000096:biosynthesis; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010100:Oxygen / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012822:Silicon Dioxide / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: D051272:Glucose Transporter Type 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D007211:Indoles; D058185:Magnetite Nanoparticles; D012333:RNA, Messenger; C495677:SLC2A1 protein, human; D019344:Lactic Acid; D012822:Silicon Dioxide; C030374:indole; D002374:Catalase; D010100:Oxygen
keywords: Glycolysis; HIF; Indole; Magnetic nanoparticle; Silica