title: Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality.
abstract: A gene encoding the high-molecular-weight (HMW) subunit of glutenin 1Ax1 was isolated from bread wheat cv Hope. Comparison of the deduced amino acid sequence with that previously reported for an allelic subunit, 1Ax2(*), showed only minor differences, which were consistent with both subunits being associated with good bread-making quality. Quantitative analyses of total protein extracts from 22 cultivars of bread wheat showed that the presence of either subunit 1Ax1 or 1Ax2(*), when compared with a null allele, resulted in an increase in the proportion of HMW subunit protein from ca. 8 to 10% of the total. It is suggested that this quantitative increase in HMW subunit protein may account for the association of 1Ax subunits with good quality.
authors.affiliation: Rothamsted Experimental Station, Institute of Arable Crops Research, AL5 2JQ, Harpenden, Herts, UK.
authors.forename: N G
authors.initials: NG
authors.lastname: Halford
authors.forename: J M
authors.initials: JM
authors.lastname: Field
authors.forename: H
authors.initials: H
authors.lastname: Blair
authors.forename: P
authors.initials: P
authors.lastname: Urwin
authors.forename: K
authors.initials: K
authors.lastname: Moore
authors.forename: L
authors.initials: L
authors.lastname: Robert
authors.forename: R
authors.initials: R
authors.lastname: Thompson
authors.forename: R B
authors.initials: RB
authors.lastname: Flavell
authors.forename: A S
authors.initials: AS
authors.lastname: Tatham
authors.forename: P R
authors.initials: PR
authors.lastname: Shewry