title: [Effect of laminarin polysaccharide on activity of matrix metalloproteinase in photoaging skin].
abstract: OBJECTIVE
  To study the effect of laminarin polysaccharide (LP) on the activity of matrix metalloproteinase of photoaging skins.
  
  
  METHODS
  Kunming SPF mice were prepared with back hair shaved, and randomly divided into the control group, the model group, the LP low does group (LP-L, 1 mg x kg(-1)), the LP high dose group (LP-H, 5 mg x kg(-1)) and the Vit E (100 mg x kg(-1)) group. They were abdominally injected with drugs twice on a daily basis. Except for the control group, all groups were exposed to ultraviolet rays for 1 hour every day, five times on a weekly basis, with accumulated exposure dose of UVB being 21.60 J x cm(-2) and accumulated exposure dose of UVA being 84.02 J x cm(-2). Eight weeks later, exposed back skins were collected to detect thickness of dermis by HE stain, content of hydroxyproline (Hyp) by chemical colorimetry, and serum MMP-1 and TIMP-1 content by ELISA. In addition, matrix metalloproteinase-1 (MMP-1) mRNA and relative content of tissue inhibitor of metalloproteinase-1 (TIMP1) mRNA was analyzed with Real-time PCR.
  
  
  RESULTS
  Compared with the model group, the LP-H group could significantly increase the thickness of dermis, skin Hyp content and serum TIMP-1 level, and decrease relative content of MMP-1 mRNA in skin and MMP-1 content in serum.
  
  
  CONCLUSIONS
  LP can regulate the metabolism of collagen photoaging skins by adjusting the activity of matrix metalloproteinase.
authors.affiliation: Guangxi Medical University, Department of Physiology, Nanning 530021, China. lijing@gxmu.edu.cn
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Lu
authors.initials: L
authors.lastname: Xie
authors.forename: Yu
authors.initials: Y
authors.lastname: Qin
authors.forename: Wei-Heng
authors.initials: WH
authors.lastname: Liang
authors.forename: Man-Qi
authors.initials: MQ
authors.lastname: Mo
authors.forename: Shi-Liang
authors.initials: SL
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Liang
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.forename: Wu
authors.initials: W
authors.lastname: Tan
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
mesh_terms: D000818:Animals; D005260:Female; D005936:Glucans; D053509:Matrix Metalloproteinase 13 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015595:Skin Aging / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D005936:Glucans; D010936:Plant Extracts; D011134:Polysaccharides; D012333:RNA, Messenger; C560825:Timp1 protein, mouse; D019715:Tissue Inhibitor of Metalloproteinase-1; C008247:laminaran; D053509:Matrix Metalloproteinase 13; C507081:Mmp13 protein, mouse