title: A multiplexed nucleic acid microsystem for point-of-care detection of HIV co-infection with MTB and PCP.
abstract: Many individuals infected with the human immunodeficiency virus (HIV), especially children in African countries, die of co-infections with Mycobacterium tuberculosis (MTB) (coinfection rate: 50%) or Pneumocystis carinii pneumonia (PCP) (coinfection rate: 81%). The present proposal describes a rapid, portable, low-cost, multiplexed point-of-care diagnostic technique for simultaneously detecting HIV, MTB, and PCP. This technique incorporates a creative micro-device (hardware) and a loop-mediated isothermal amplification strategy (software).
authors.affiliation: Department of Chemistry, Fudan University, Shanghai 200433, China.
authors.forename: Lingjia
authors.initials: L
authors.lastname: Xu
authors.forename: Jilie
authors.initials: J
authors.lastname: Kong
mesh_terms: D002648:Child; D060085:Coinfection; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D015658:HIV Infections / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques; D010957:Plasmids / Q000737:chemistry; D011020:Pneumonia, Pneumocystis / Q000175:diagnosis* / Q000382:microbiology; D019095:Point-of-Care Systems; D012680:Sensitivity and Specificity; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D004271:DNA, Fungal; D004279:DNA, Viral
keywords: Human immunodeficiency virus (HIV); Loop-mediated isothermal amplification (LAMP); M. tuberculosis (MTB); Microfluidics; Pneumocystis carinii pneumonia (PCP)