title: Quantitative detection and proliferation dynamics of a novel Spiroplasma eriocheiris pathogen in the freshwater crayfish, Procambarus clarkii.
abstract: Spiroplasma eriocheiris disease control based on sensitive quantitative methods has become a priority. A SYBR Green real-time PCR that can simultaneously detect and quantify S. eriocheiris in the freshwater crayfish Procambarus clarkii was produced and evaluated. In the asymptomatic crayfish, hemolymph exhibited the statistically greatest number of S. eriocheiris copies indicating a tissue-specific pathogen infection characteristic. The curve of the pathogen amount change in vivo assumed a very similar shape with the typical one-step growth curve. A turning point from chronic infection to acute infection was suggested from 3 to 4 days when the S. eriocheiris copies in hemolymph increased substantially.
authors.affiliation: Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210046, China; Freshwater Fisheries Research Institute of Jiangsu Province, 79 Chating East Street, Nanjing 210017, China.
authors.forename: Zhengfeng
authors.initials: Z
authors.lastname: Ding
authors.affiliation: Freshwater Fisheries Research Institute of Jiangsu Province, 79 Chating East Street, Nanjing 210017, China.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Tang
authors.affiliation: Freshwater Fisheries Research Institute of Jiangsu Province, 79 Chating East Street, Nanjing 210017, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xue
authors.affiliation: Freshwater Fisheries Research Institute of Jiangsu Province, 79 Chating East Street, Nanjing 210017, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210046, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ren
authors.affiliation: Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210046, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
authors.affiliation: Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210046, China. Electronic address: mlzzcld@gmail.com.
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210046, China. Electronic address: njnuwang@263.net.
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
mesh_terms: D000818:Animals; D003400:Astacoidea / Q000382:microbiology*; D005618:Fresh Water; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013149:Spiroplasma / Q000166:cytology
keywords: Quantitation; Real-time PCR; SYBR Green; Spiroplasma eriocheiris