title: Comparison of two nested PCR methods for the detection of human malaria.
abstract: Battling malaria will be a persistent struggle without the proper means to diagnose the parasitic infection. However, the inherent limitations of microscopy, the conventional method of diagnosing malaria, affect the accuracy of diagnosis. The present study aimed to compare the accuracy of two different set of primers targeting the small subunit ribosomal RNA (ssRNA) and the dihydrofolate reductase-thymidylate synthase linker region (dhfr-ts) in detecting species specific malaria infections by nested PCR. The sensitivity and specificity of nested PCR assay using the two primers were calculated with reference to microscopy as the 'gold standard'. The results show that 18S rRNA primers had 91.9% sensitivity and 100% specificity in detecting human Plasmodium species as opposed to dhfr-ts primers which had 51.4% sensitivity and 100% specificity. The higher sensitivity of 18S rRNA primers suggests that it may be a better diagnostic tool for detecting human malaria.
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: C
authors.initials: C
authors.lastname: Anthony
authors.forename: R
authors.initials: R
authors.lastname: Mahmud
authors.forename: Y L
authors.initials: YL
authors.lastname: Lau
authors.forename: S F
authors.initials: SF
authors.lastname: Syedomar
authors.forename: S
authors.initials: S
authors.lastname: Sri La Sri Ponnampalavanar
mesh_terms: D017931:DNA Primers / Q000235:genetics; D021901:DNA, Intergenic; D006801:Humans; D008288:Malaria / Q000175:diagnosis*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D010961:Plasmodium / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012335:RNA, Ribosomal / Q000235:genetics; D012680:Sensitivity and Specificity; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D013940:Thymidylate Synthase / Q000235:genetics
chemical_list: D017931:DNA Primers; D021901:DNA, Intergenic; D012335:RNA, Ribosomal; D013762:Tetrahydrofolate Dehydrogenase; D013940:Thymidylate Synthase