title: Factors influencing the efficiency of T-DNA transfer during co-cultivation of Antirrhinum majus with Agrobacterium tumefaciens.
abstract: The effects of varying the pH of the cocultivation medium, additons of vir-inducing phenolic compounds and the strains of wild-type agrobacteria on transformation rates of a number of different varieties of Antirrhinum majus were studied. In general, optimal transformation was found with strains C58 or A281 and was favoured by low pH and the inclusion of acetosyringone in the co-cultivation medium. However, maximal transformation of the least susceptible variety was achieved at high pH and in the presence of syringaldehyde. This demonstrates the need for the optimization of a wide range of culture conditions when working with new genotypes and offers a rational approach towards the development of Agrobacterium-mediated transformation of new species or varieties.
authors.affiliation: School of Biological Sciences, University of Birmingham, B15 2TT, Birmingham, UK.
authors.forename: P
authors.initials: P
authors.lastname: Holford
authors.forename: N
authors.initials: N
authors.lastname: Hernandez
authors.forename: H J
authors.initials: HJ
authors.lastname: Newbury