title: Tumor-suppressive microRNA-449a induces growth arrest and senescence by targeting E2F3 in human lung cancer cells.
abstract: MicroRNA-449a (miR-449a) was significantly downregulated in 156 lung cancer tissues (p<0.001). We found that the low expression of miR-449a was highly correlated with cancer recurrence and survival of lung cancer patients. The transient introduction of miR-449a caused cell cycle arrest and cell senescence in A549 and 95D cells. Further studies revealed that E2F3 was a direct target of miR-449a in lung cancer cells. miR-449a also suppressed tumor formation in vivo in nude mice. These results suggest that miR-449a plays an important role in lung cancer tumorigenesis and that miR-449a might predict cancer recurrence and survival of lung cancer patients.
authors.affiliation: The State Key Laboratory of Respiratory, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; University of Science and Technology of China, Hefei 230026, China.
authors.forename: Xiao-Shuai
authors.initials: XS
authors.lastname: Ren
authors.affiliation: The State Key Laboratory of Respiratory, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; University of Science and Technology of China, Hefei 230026, China.
authors.forename: Meng-Hui
authors.initials: MH
authors.lastname: Yin
authors.affiliation: Department of Cardiothoracic Surgery, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: The State Key Laboratory of Respiratory, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Guangzhou RiboBio Co., Ltd., Guangzhou 510663, China.
authors.forename: Shi-Peng
authors.initials: SP
authors.lastname: Feng
authors.affiliation: The State Key Laboratory of Respiratory, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Guo-Xin
authors.initials: GX
authors.lastname: Wang
authors.affiliation: Guangzhou RiboBio Co., Ltd., Guangzhou 510663, China.
authors.forename: Ying-Jun
authors.initials: YJ
authors.lastname: Luo
authors.affiliation: The State Key Laboratory of Respiratory, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Graduate University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Pei-Zhou
authors.initials: PZ
authors.lastname: Liang
authors.affiliation: Guangzhou RiboBio Co., Ltd., Guangzhou 510663, China.
authors.forename: Xiu-Qun
authors.initials: XQ
authors.lastname: Yang
authors.affiliation: Department of Cardiothoracic Surgery, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China. Electronic address: drjianxing.he@gmail.com.
authors.forename: Jian-Xing
authors.initials: JX
authors.lastname: He
authors.affiliation: The State Key Laboratory of Respiratory, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; University of Science and Technology of China, Hefei 230026, China. Electronic address: zhang_biliang@gibh.org.
authors.forename: Bi-Liang
authors.initials: BL
authors.lastname: Zhang
mesh_terms: D000818:Animals; D063646:Carcinogenesis / Q000235:genetics; D059447:Cell Cycle Checkpoints / Q000235:genetics; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D016922:Cellular Senescence / Q000235:genetics; D050699:E2F3 Transcription Factor / Q000235:genetics*; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D014162:Transfection
chemical_list: D050699:E2F3 Transcription Factor; C098319:E2F3 protein, human; C544530:MIRN449 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering
keywords: E2F3; Lung cancer; Senescence; Survival; miR-449a