title: [Effect of HMGB1 on invasion and migration of human hepatoma cell line HepG2 and its mechanism].
abstract: OBJECTIVE
  To investigate the effect of high mobility group-box protein 1 (HMGB1)-siRNA on invasion and migration of human hepatoma cell line HepG2, and further to explore its mechanism.
  
  
  METHODS
  HMGB1-siRNA was synthesized with RNA interference and transferred into HepG2 cells to down-regulate the expression of HMGB1. The invasion and migration activities were assayed by Transwell™ assay and monolayer wounding healing assay. The levels of matrix metalloproteinase 2 (MMP-2), MMP-9, intercellular adhesion molecule 1 (ICAM-1) and tissue inhibitor of MMP-2 (TIMP-2) were detected by RT-PCR and Western blotting in HepG2 cells after treatment with 40 nmol/L HMGB1-siRNA for 24 h.
  
  
  RESULTS
  The migration and invasion abilities of HepG2 cells were inhibited by 40 nmol/L HMGB1-siRNA markedly. Compared with the control group, MMP-2, MMP-9, ICAM-1 were down-regulated, TIMP-2 was up-regulated significantly by HMGB1-siRNA (P<0.05).
  
  
  CONCLUSIONS
  HMGB1-siRNA can inhibit the invasion and migration abilities of human hepatoma cells by down-regulating the expressions of MMP-2, MMP-9, ICAM-1 and up-regulating the expression of TIMP-2.
authors.affiliation: School of Graduates, General Hospital, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.forename: Chaofeng
authors.initials: C
authors.lastname: Tang
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Chang
authors.forename: Zhaoyu
authors.initials: Z
authors.lastname: Li
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002465:Cell Movement; D015972:Gene Expression Regulation, Neoplastic; D024243:HMGB1 Protein / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D008113:Liver Neoplasms / Q000473:pathology*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics / Q000378:metabolism
chemical_list: D024243:HMGB1 Protein; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D018799:Intercellular Adhesion Molecule-1; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9