title: Citrate transport into barley mesophyll vacuoles - comparison with malate-uptake activity.
abstract: Citrate uptake into barley (Hordeum vulgare L.) mesophyll vacuoles was found to be saturable with a K m of about 200 μM. Uptake appears to occur via the citrate(3-) form, as indicated by concentration-dependent uptake studies at different pHs. Free citrate and not the Mg-citrate complex was taken up by the vacuoles, even though slow transport of the Mg complex could not be excluded. Citrate transport into vacuoles was competitively inhibited by malate (K i=0.68 mM). Various organic acids and protein-modifying agents affected the uptake of malate and citrate to a similar extent. These results indicate that both organic acids cross the tonoplast by means of the same carrier. Accumulation of citrate was ATP-dependent and could be inhibited by ionophores. Bovine serum albumin strongly stimulated citrate uptake, but other proteins tested did not show a similar stimulatory effect.
authors.affiliation: Swiss Federal Institute of Technology, Institute of Plant Sciences, ETH-Zentrum, Sonneggstrasse 5, CH-8092, Zürich, Switzerland.
authors.forename: D
authors.initials: D
authors.lastname: Rentsch
authors.forename: E
authors.initials: E
authors.lastname: Martinoia