title: Cryopreservation of embryonic axes of trifoliate orange (Poncirus trifoliata [L.] RAF.).
abstract: Seeds of trifoliate orange (Poncirus trifoliata (L.) Raf.) are sensitive to desiccation, and could not withstand reduction in moisture level below 20%, whereas the excised embryonic axes could be easily desiccated to moisture levels as low as 14% without much loss in viability. Axes could be successfully cryopreserved in liquid nitrogen (-196°C) for eight months. The viable embryonic axes exhibited good growth on modified Murashige and Skoog medium supplemented wiith 1-Naphthalene acetic acid (NAA) and 6-Benzylaminopurine (BAP). Growth of cryopreserved axes was promoted in the presence of charcoal in the medium allowing for plant recovery.
authors.affiliation: National Plant Tissue Culture Repository, NBPGR Campus, 110012, New Delhi, India.
authors.forename: J
authors.initials: J
authors.lastname: Radhamani
authors.forename: K P
authors.initials: KP
authors.lastname: Chandel