title: Somatic embryogenesis and plant regeneration in Quercus acutissima.
abstract: Immature embryos of Quercus acutissima were collected weekly beginning 5 weeks post-fertilization and cultured on modified MS(Murashige and Skoog) medium containing 1,000 mg/l glutamine and 5 mM proline with different combinations of IBA(0.5-10.0 mg/l) and BA(0 or 1.0 mg/l) in light. The highest percentage of embryogenic cultures occurred on the medium containing 0.5 mg/l IBA or 1.0 mg/l BA and 0.5 mg/l IBA. Four weeks after initiation, the embryogenic cultures were transferred to MS medium without plant growth regulators and cultured for 4 weeks. The somatic embryos were then transferred to germination medium. The best germination results were achieved from WPM(Woody Plant Medium) containing 0.1 mg/l BA. Plantlets from somatic embryos were incubated on WPM supplemented with 0.2 mg/l BA for 4 weeks and plantlets with well developed shoots and roots were transplanted to perlite and peat moss(1∶1, v/v) mixtures and placed in a culture room. After being hardened off for 8 weeks, they were transferred outdoors where they grew.
authors.affiliation: Forest Genetics Research Institute, P.O. Box 24, Suwon, Kyeonggido, Republic of Korea.
authors.forename: Y W
authors.initials: YW
authors.lastname: Kim
authors.forename: B C
authors.initials: BC
authors.lastname: Lee
authors.forename: S K
authors.initials: SK
authors.lastname: Lee
authors.forename: S S
authors.initials: SS
authors.lastname: Jang