title: Production of bioactive compounds with antitumor activity against sarcoma 180 by Pleurotus sajor-caju.
abstract: This work studied the influence of culture medium composition and pH on exopolysaccharide (EPS) production by Pleurotus sajor-caju and validates the antitumor activity of the produced EPSs and of the mycelial biomass (intracellular polysaccharides [IPSs]) against Sarcoma 180 (S180) cells. The effect of the initial concentrations of (NH₄)₂SO₄, yeast extract and soy peptone on EPS production by P. sajor-caju was studied in shake flasks. A bioreactor was used to evaluate the pH values and the initial CaCO₃ and glucose concentrations. Extracts of EPSs (PE1) and IPSs obtained through two different separation processes (PM1 and PM2) were tested on mice inoculated with S180 cells. A medium containing 2.5, 1.0, and 1.0 g/L of (NH₄)₂SO₄, yeast extract and soy peptone, respectively, provided the highest EPS concentration (0.6 g/L). The use of pH 4.0, 1.0 g/L CaCO₃ and 20 g/L initial glucose concentration enhanced EPS productivity (3.84 g/L per hour). The PE1 extract promoted the highest reduction of S180 growth (86%), followed by the PM2 extract (80%); growth reduction was dose-independent for both substances. This work provides information about culture medium and conditions that enhanced the production of extracellular polysaccharides by P. sajor-caju. The results can contribute to the search for new bioactive products bringing novel aspects to the medical and pharmaceutical areas.
authors.affiliation: 1 Master's Program in Health and Environment, University of the Joinville Region (Univille) , Joinville, Santa Catarina, Brazil .
authors.forename: Ivaneliza Simionato
authors.initials: IS
authors.lastname: Assis
authors.forename: Mariane Bonatti
authors.initials: MB
authors.lastname: Chaves
authors.forename: Marcia Luciane Lange
authors.initials: ML
authors.lastname: Silveira
authors.forename: Regina Maria Miranda
authors.initials: RM
authors.lastname: Gern
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Wisbeck
authors.forename: Agenor Furigo
authors.initials: AF
authors.lastname: Júnior
authors.forename: Sandra Aparecida
authors.initials: SA
authors.lastname: Furlan
mesh_terms: D000645:Ammonium Sulfate; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001688:Biological Products / Q000494:pharmacology / Q000627:therapeutic use*; D019149:Bioreactors; D003470:Culture Media / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D025282:Mycelium; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000494:pharmacology / Q000627:therapeutic use*; D012510:Sarcoma 180 / Q000188:drug therapy*; D030262:Soybean Proteins; D015003:Yeasts
chemical_list: D000970:Antineoplastic Agents; D001688:Biological Products; D003470:Culture Media; D011134:Polysaccharides; D030262:Soybean Proteins; D000645:Ammonium Sulfate