title: Impact of application of bio-amniotic membrane immersed in 5-fluorouracil solution in trabeculectomy on rabbit retina.
abstract: BACKGROUND
  To observe the impact of application of bio-amniotic membrane immersed in 5-fluorouracil solution in trabeculectomy on the retina in a rabbit model.
  
  
  METHODS
  Healthy white New Zealand rabbits were randomly assigned into three groups with 20 in each group. Bio-amniotic membranes of 4 × 5 mm immersed in either physiological saline/water for 10 min, or 25 mg/mL 5-fluorouracil solution for 5 and 10 min, respectively, were applied on rabbit eyes during trabeculectomy. At 7, 14, 21, and 28 days of postoperation, five rabbits from each group were examined with electroretinogram (ERG). After being examined for eye pressure and bleb morphology, rabbits were sacrificed by air embolism and their retinas were collected and examined by transmission electron microscopy (TEM). In addition, 5-fluorouracil amount in bio-amniotic membranes was measured using high-performance liquid chromatography.
  
  
  RESULTS
  Each bio-amniotic membrane could absorb 59.004 μg and 75.828 μg 5-fluorouracil after being immersed in 5-fluorouracil solution for 5 and 10 min, respectively. Application of these bio-amniotic membranes in trabeculectomy could promote the formation of well-functioning bleb and maintain intraocular pressure, although it had no effect on retina structures as examined with ERG and TEM.
  
  
  CONCLUSIONS
  Application of 5-FU soaked bio-amniotic membrane in rabbit eye trabeculectomy is effective and safe.
authors.forename: Chenming
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Ophthalmology, Qilu Hospital of Shandong University, Jinan 250012, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Jianrong
authors.initials: J
authors.lastname: Wang
authors.forename: Bailin
authors.initials: B
authors.lastname: Guo
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000650:Amnion / Q000637:transplantation; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005472:Fluorouracil / Q000008:administration & dosage*; D005901:Glaucoma / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D007166:Immunosuppressive Agents / Q000008:administration & dosage; D007429:Intraocular Pressure; D007432:Intraoperative Period; D008297:Male; D009883:Ophthalmic Solutions; D011817:Rabbits; D012160:Retina / Q000187:drug effects* / Q000473:pathology; D014130:Trabeculectomy / Q000379:methods*
chemical_list: D007166:Immunosuppressive Agents; D009883:Ophthalmic Solutions; D005472:Fluorouracil