title: Production of valepotriates by hairy root cultures of Centranthus ruber DC.
abstract: Hairy root cultures of Centranthus ruber DC. were established by infection of sterile plantlets with Agrobacterium rhizogenes, strain R1601. The transformed roots were grown in 12 different, hormone-free liquid media, and valtrate, isovaltrate, 7-desisovaleroyl-7-acetylvaltrate, 7-homovaltrate, didrovaltrate and isovaleroxyhydroxydidrovaltrate were quantified by high performance liquid chromatography. The highest overall valepotriate content (3.0% dry wt) was observed in half-strength Gamborg B5 medium supplemented with 3% sucrose. This concentration is very similar to that found in the roots of parent plants grown in the field. The use of N,N-dimethylmorpholinium iodide, a plant bioregulator, was very detrimental to the hairy root growth and to the valepotriate production. The hairy roots cultured in half strength Gamborg B5 liquid medium supplemented with 3% sucrose for 45 days produced over 31 mg/g dry wt valepotriates.
authors.affiliation: Department of Pharmacognosy, University of Geneva, Sciences II, 30, Quai Ernest-Ansermet, CH-1211, 4, Geneva, Switzerland.
authors.forename: F
authors.initials: F
authors.lastname: Gränicher
authors.forename: P
authors.initials: P
authors.lastname: Christen
authors.forename: I
authors.initials: I
authors.lastname: Kapétanidis