title: Immune response of heifers against a Staphylococcus aureus CP5 whole cell and lysate vaccine formulated with ISCOM Matrix adjuvant.
abstract: Staphylococcus aureus is the most frequently isolated pathogen from bovine intramammary infections worldwide. Commercially available vaccines for mastitis control are composed either of S. aureus lysates or whole-cells formulated with traditional adjuvants. We recently showed the ability of a S. aureus CP5 whole-cell vaccine adjuvanted with ISCOM Matrix to increase specific antibodies production in blood and milk, improving opsonic capacity, compared with the same vaccine formulated with Al(OH)3. However, there is no information about the use of ISCOM Matrix for the formulation of bacterial lysates. The aim of this study was to characterize the innate and humoral immune responses induced by a S. aureus CP5 whole-cell or lysate vaccine, formulated with ISCOM Matrix after immunization of pregnant heifers. Both immunogens stimulated strong humoral immune responses in blood and milk, raising antibodies that increased opsonic capacity. Lysate formulation generated a higher and longer lasting antibody titer and stimulated a higher expression of regulatory and pro-inflammatory cytokines compared with the whole-cell vaccine.
authors.affiliation: E.E.A. Rafaela, INTA, Santa Fe, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina.
authors.forename: Cecilia M
authors.initials: CM
authors.lastname: Camussone
authors.affiliation: Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
authors.forename: Carolina M
authors.initials: CM
authors.lastname: Veaute
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina; Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
authors.forename: Nazarena
authors.initials: N
authors.lastname: Pujato
authors.affiliation: Department of Clinical Virology, Uppsala University, Sweden.
authors.forename: Bror
authors.initials: B
authors.lastname: Morein
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina; Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
authors.forename: Iván S
authors.initials: IS
authors.lastname: Marcipar
authors.affiliation: E.E.A. Rafaela, INTA, Santa Fe, Argentina; Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Esperanza, Santa Fe, Argentina. Electronic address: calvinho.luis@inta.gob.ar.
authors.forename: Luis F
authors.initials: LF
authors.lastname: Calvinho
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology*; D002417:Cattle; D015169:Colony Count, Microbial / Q000662:veterinary; D016207:Cytokines / Q000097:blood / Q000235:genetics; D005260:Female; D016882:ISCOMs / Q000494:pharmacology*; D007114:Immunization / Q000592:standards / Q000662:veterinary; D008414:Mastitis, Bovine / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D008892:Milk / Q000382:microbiology; D011247:Pregnancy; D012313:RNA / Q000737:chemistry / Q000235:genetics; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013203:Staphylococcal Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000235:genetics / Q000276:immunology*; D018709:Statistics, Nonparametric
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D016207:Cytokines; D016882:ISCOMs; D012313:RNA
keywords: ISCOM Matrix; Immunization; Lysate; Mastitis; Staphylococcus aureus; Whole cell