title: Novel method of the synthesis and hybridization properties of an oligonucleotide containing non-ionic diisopropylsilyl internucleotide linkage.
abstract: Efficient synthesis of a dithymidine dinucleotide analog bearing a diisopropylsilyl linkage instead of a phosphodiester linkage is described with respect to its incorporation into oligonucleotides. The diisopropylsilyl linkage was introduced into the oligonucleotide by preparation of the phosphoramidite derivative of a dithymidine dimer unit. The diisopropylsilyl-modified oligonucleotide exhibited hybridization behavior with both single strand and duplex DNA. The thermal stability of both the duplex and triplex showed a relative instability compared to the corresponding natural phosphodiester DNA, because of the steric hindrance of the isopropyl group on the silicon atom.
authors.affiliation: Division of Molecular Science, Faculty of Science and Technology, Gunma University, Tenjincho 1-5-1, Kiryu, Gunma, Japan. Electronic address: moriguchi@gunma-u.ac.jp.
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Moriguchi
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Sekine
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozuka
mesh_terms: D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D012821:Silanes
keywords: Diisopropylsilyl linkage; Duplex formation; Oligonucleotides; Phosphodiester linkage; Triplex formation