title: Identification and characterization of metabolic properties of bacterial populations recovered from arsenic contaminated ground water of North East India (Assam).
abstract: Diversity of culturable bacterial populations within the Arsenic (As) contaminated groundwater of North Eastern state (Assam) of India is studied. From nine As contaminated samples 89 bacterial strains are isolated. 16S rRNA gene sequence analysis reveals predominance of Brevundimonas (35%) and Acidovorax (23%) along with Acinetobacter (10%), Pseudomonas (9%) and relatively less abundant (<5%) Undibacterium, Herbaspirillum, Rhodococcus, Staphylococcus, Bosea, Bacillus, Ralstonia, Caulobacter and Rhizobiales members. High As(III) resistance (MTC 10-50 mM) is observed for the isolates obtained from As(III) enrichment, particularly for 3 isolates of genus Brevundimonas (MTC 50 mM). In contrast, high resistance to As(V) (MTC as high as 550 mM) is present as a ubiquitous property, irrespective of isolates' enrichment condition. Bacterial genera affiliated to other groups showed relatively lower degree of As resistance [MTCs of 15-20 mM As(III) and 250-350 mM As(V)]. As(V) reductase activity is detected in strains with high As(V) as well as As(III) resistance. A strong correlation could be established among isolates capable of reductase activity and siderophore production as well as As(III) tolerance. A large number of isolates (nearly 50%) is capable of anaerobic respiration using alternate inorganic electron acceptors [As(V), Se(VI), Fe(III), [NO(3)(2), SO(4)(2), S(2)O(3)(2). Ability to utilize different carbon sources ranging from C2-C6 compounds along with some complex sugars is also observed. Particularly, a number of strains is found to possess ability to grow chemolithotrophically using As(III) as the electron donor. The study reports for the first time the identity and metabolic abilities of bacteria in As contaminated ground water of North East India, useful to elucidate the microbial role in influencing mobilization of As in the region.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, 721302 West Bengal, India.
authors.forename: Soma
authors.initials: S
authors.lastname: Ghosh
authors.forename: Pinaki
authors.initials: P
authors.lastname: Sar
mesh_terms: D001151:Arsenic / Q000032:analysis* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D042063:Caulobacteraceae / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D042621:Comamonadaceae / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D060587:Groundwater / Q000032:analysis / Q000382:microbiology*; D007194:India; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D001151:Arsenic
keywords: Acidovorax; Acinetobacter; Arsenic contamination; Assam; Biogeochemical cycling; Brevundimonas; Groundwater