title: Analysis of the effect of phototherapy in model with traumatic Achilles tendon injury in rats.
abstract: The aim of this study was to investigate the effect of low-intensity laser (LILT) infrared (830 nm) therapy in tendon inflammation, tendinitis induced by mechanical trauma in rat Achilles tendon. For this, we used 65 young male Wistar rats, weighing ± 300 g divided into different groups: C = control (n = 5) and experimental (n = 10/group), with two different times of sacrifice such as treated with L = laser, D = treated with diclofenac, and T = untreated injured. The tendon inflammation was induced by controlled contusion in the medial region of the Achilles tendon of the animals. The treated groups received some kind of intervention every 24 h, all groups were sacrificed on the 7th or 14th day after the trauma. The tendons were dissected, extracted, and sent for analysis. Histological analysis of the L group showed a decrease in the number of inflammatory cells in relation to other groups in both periods studied. The comparative results between the number of inflammatory cells in the control and treated groups at 7 and 14 days showed statistically significant differences. Qualitative analysis findings obtained by the picrosirius red technique under polarized light showed that in 7 days, the T group presented collagen types I and III in the same proportion; group D presented a predominance of type III fibers, while in group L, type I collagen predominated. The 14-day group D showed collagen types I and III in the same proportion, while in group L, there was a predominance of type I fibers. Biomechanical analysis showed that 7-day groups L and C showed similar stiffness and increased breaking strength. The 14-day groups L and C showed greater rupturing strength as well as increased stiffness angle. Group D showed a decrease of maximum traction strength and degree of rigidity. It was concluded that treatment with LIL in the parameters used and the times studied reduces migration of inflammatory cells and improves the quality of repair while reducing the functional limitations.
authors.affiliation: Post Graduate Program in Rehabilitation Sciences, Universidade Nove de Julho (UNINOVE), Rua Vergueiro, 235, São Paulo, SP, 01504-001, Brazil.
authors.forename: Heliodora Leão
authors.initials: HL
authors.lastname: Casalechi
authors.forename: Anna Cristina
authors.initials: AC
authors.lastname: de Farias Marques
authors.forename: Evela Aparecida Pereira
authors.initials: EA
authors.lastname: da Silva
authors.forename: Flávio
authors.initials: F
authors.lastname: Aimbire
authors.forename: Rodrigo Labat
authors.initials: RL
authors.lastname: Marcos
authors.forename: Rodrigo A B
authors.initials: RA
authors.lastname: Lopes-Martins
authors.forename: Paulo de Tarso Camilo
authors.initials: Pde T
authors.lastname: de Carvalho
authors.forename: Regiane
authors.initials: R
authors.lastname: Albertini
mesh_terms: D000125:Achilles Tendon / Q000293:injuries / Q000503:physiopathology / Q000528:radiation effects; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology / Q000627:therapeutic use; D001696:Biomechanical Phenomena; D024042:Collagen Type I / Q000378:metabolism; D004008:Diclofenac / Q000494:pharmacology / Q000627:therapeutic use; D028022:Low-Level Light Therapy; D008297:Male; D017208:Rats, Wistar; D052256:Tendinopathy / Q000276:immunology / Q000532:radiotherapy*; D013708:Tendon Injuries / Q000276:immunology / Q000532:radiotherapy*; D014945:Wound Healing / Q000528:radiation effects
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D024042:Collagen Type I; D004008:Diclofenac