title: KRASmutational status analysis of peripheral blood isolated circulating tumor cells in metastatic colorectal patients.
abstract: The present study describes an optimized method for isolating peripheral blood circulating tumor cells (CTCs) and performingKRASmutation analysis. The approach combines isolation of peripheral blood mononuclear cells and immunomagnetic labeling with CD45 and CD326 human microbeads with KRAS analysis performed with a Therascreen KRAS kit by quantitative PCR.KRASmutations were detected in the CTCs of patients with metastatic colorectal cancer (mCRC). CTCs may represent an alternative to invasive procedures and their analysis may be representative of the current disease status of the patient. This proposed analysis may be performed in a daily clinical practice.
authors.affiliation: Clinical Genetics Unit, University Clinic of Navarra, Pamplona, Navarra 31008, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Gutiérrez
authors.forename: Javier
authors.initials: J
authors.lastname: Rodriguez
authors.forename: Ana
authors.initials: A
authors.lastname: Patiño-García
authors.forename: Jesús
authors.initials: J
authors.lastname: García-Foncillas
authors.forename: Josefa
authors.initials: J
authors.lastname: Salgado
keywords: KRAS gene; circulating tumor cells; inmunomagnetic labeling; metastatic colorectal cancer; peripheral blood