title: A protoplast to plant system for the chrysanthemum Dendranthema zawadskii x D. grandiflora.
abstract: Plantlets were regenerated from protoplasts of in vitro shoot cultures and leaf-derived de novo shoots of the chrysanthemum Dendranthema zawadskii x D. grandiflora. Isolated protoplasts reformed cell walls and then began to divide within 24 hours of culture in streaky plate agarose lenses surrounded by liquid V-KM medium. Twenty one days after isolation, 1 mm diameter callus clumps were transferred to shoot regeneration medium. After a further 33 days leaves became visible. Elongated shoots were rooted on half strength hormone-free MS medium.
authors.affiliation: MAF Technology, Ministry of Agriculture and Fisheries, Horticultural Research Centre, Private Bag, Levin, New Zealand.
authors.forename: G C
authors.initials: GC
authors.lastname: Lindsay
authors.forename: S E
authors.initials: SE
authors.lastname: Ledger