title: Crystallization and preliminary X-ray diffraction studies of the reduced form of the terminal oxygenase component of the Rieske nonhaem iron oxygenase system carbazole 1,9a-dioxygenase.
abstract: The initial reaction of bacterial carbazole degradation is catalysed by carbazole 1,9a-dioxygenase, which consists of terminal oxygenase, ferredoxin and ferredoxin reductase components. The reduced form of the terminal oxygenase component was crystallized at 293 K by the hanging-drop vapour-diffusion method using PEG MME 550 as the precipitant under anaerobic conditions. The crystals diffracted to a resolution of 1.74 Å and belonged to space group P6(5), with unit-cell parameters a = b = 92.0, c = 243.6 Å. The asymmetric unit contained a trimer of terminal oxygenase molecules.
authors.affiliation: Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Matsuzawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Umeda
authors.forename: Hiroki
authors.initials: H
authors.lastname: Aikawa
authors.forename: Chiho
authors.initials: C
authors.lastname: Suzuki
authors.forename: Zui
authors.initials: Z
authors.lastname: Fujimoto
authors.forename: Kazunori
authors.initials: K
authors.lastname: Okada
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Yamane
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nojiri
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D049308:Dioxygenases / Q000737:chemistry*; D014450:Electron Transport Complex III / Q000737:chemistry*; D004795:Enzyme Stability; D007501:Iron / Q000378:metabolism*; D010084:Oxidation-Reduction; D020560:Proteobacteria / Q000201:enzymology*; D013057:Spectrum Analysis; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; C037989:Rieske iron-sulfur protein; D007501:Iron; D049308:Dioxygenases; C108434:carbazole 1,9a-dioxygenase; D014450:Electron Transport Complex III
keywords: Janthinobacterium sp. J3; Rieske nonhaem iron oxygenase; carbazole 1,9a-dioxygenase; reduced form; terminal oxygenase component