title: [Effect of tanshinone II A on the expressions of NeuN, GFAP and CD11b in Aβ1-42; induced newborn rat hippocampal slices in vitro].
abstract: OBJECTIVE
  To detect the expressions of neuron-specific nuclear protein (NeuN), glial fibrillary acidic protein (GFAP) and CD11b in the Aβ1-42 induced newborn rat hippocampal slices in vitro and investigate the neuroprotective effect of tanshinone IIA.
  
  
  METHODS
  Hippocampal slices were randomly divided into 7 groups: normal control group; Aβ1-42 (5.0, 0.5 μg/mL) groups; Aβ (5.0 μg/mL) + low or high (8.0 mg or 16 mg) doses of Tan IIA groups, Aβ (0.5 μg/mL) + low or high (8.0 mg or 16 mg) doses of Tan IIA groups. The expression levels of GFAP, NeuN and CD11b were detected by immunohistochemistry.
  
  
  RESULTS
  Compared with the control group, the expression of NeuN decreased in the two different doses of Aβ1-42 groups, especially in the high-dose group (P<0.05); while the level significantly increased after high-dose Tan IIA treatment (P<0.05). Compared with the control group, the expression levels of GFAP and CD11b increased in the two different doses of Aβ1-42 groups, and the levels in the high-dose group were the highest (P<0.05); while the levels were reduced significantly by Tan IIA treatment, the more by the high-dose Tan IIA (P<0.05).
  
  
  CONCLUSIONS
  Tan IIA can down-regulate the levels of GFAP and CD11b and inhibit the activity of glial cells in rat hippocampal slices induced by Aβ1-42 in vitro.
authors.affiliation: College of Life Science, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Yanping
authors.initials: Y
authors.lastname: Liu
mesh_terms: D045784:Abietanes / Q000494:pharmacology*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D034961:Antigens, Nuclear / Q000378:metabolism*; D039481:CD11b Antigen / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000494:pharmacology; D010446:Peptide Fragments / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D045784:Abietanes; D016229:Amyloid beta-Peptides; D034961:Antigens, Nuclear; D039481:CD11b Antigen; D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; D018696:Neuroprotective Agents; D010446:Peptide Fragments; C546339:Rbfox3 protein, rat; C075222:amyloid beta-protein (1-42); C021751:tanshinone