title: Regulated changes in the acetylation of α-tubulin on lys(40) during growth and organ development in fast plants, Brassica rapa L.
abstract: Recently, we confirmed the widespread occurrence of α-tubulin acetylation on Lys(40) in angiosperms. In the present study, we found that α-tubulin acetylation is regulated in a growth stage- and organ development-dependent manner in the rapid cycling Brassica rapa, also known as Fast Plants. Organ distribution analysis showed that the proportion of acetylated α-tubulin is high in the cotyledons of young plants and in the true leaves and flowers of mature plants. A correlation between the increase in the levels of α-tubulin acetylation and the maturation of true leaves was observed. In the mature leaves, the acetylated α-tubulin showed an uneven distribution pattern, and the cells in the region of the leaf margins contained a high proportion of acetylated α-tubulin. These results indicate that α-tubulin acetylation is dynamically regulated in plant organs during development, and that it might play an important role in microtubule functioning throughout the angiosperm's life cycle.
authors.affiliation: Department of Bioscience, Nagahama Institute of Bio-Science and Technology.
authors.forename: Umihito
authors.initials: U
authors.lastname: Nakagawa
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kamemura
authors.forename: Aya
authors.initials: A
authors.lastname: Imamura
mesh_terms: D000107:Acetylation; D029741:Brassica rapa / Q000235:genetics / Q000378:metabolism*; D018548:Cotyledon / Q000235:genetics / Q000378:metabolism*; D035264:Flowers / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D008239:Lysine / Q000378:metabolism; D009928:Organ Specificity; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D014404:Tubulin / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D014404:Tubulin; D008239:Lysine