title: Enhancement of glucose uptake in muscular cell by soybean charged peptides isolated by electrodialysis with ultrafiltration membranes (EDUF): activation of the AMPK pathway.
abstract: Soy peptides consumption has been associated with beneficial effects in type 2 diabetes patients. However, the peptide fractions responsible for these effects, and their mechanisms of action, have not been identified yet. In this study, we have isolated soybean peptides by electrodialysis with an ultrafiltration membrane (EDUF) at 50 V/100 kDa, and tested them for their capacity to improve glucose uptake in L6 muscle cells. We observed that these fractions were able to significantly enhance glucose uptake in the presence of insulin. The reported bioactivity would be due to the low molecular weight peptides (300-500 Da) recovered. Moreover, we observed that an enhancement of glucose uptake was correlated to the activation of the AMPK enzyme, well known for its capacity to increase glucose uptake in muscle cells. To our knowledge, this is the first time that bioactive peptides with glucose uptake activity have been isolated from a complex soy matrix, and that the implication of AMPK in it is demonstrated.
authors.affiliation: Department of Food Sciences and Nutrition, Université Laval, Quebec, QC G1V 0A6, Canada; Institute of Nutrition and Functional Foods (INAF), Université Laval, Quebec, QC G1V 0A6, Canada. Electronic address: Cyril.roblet.1@ulaval.ca.
authors.forename: Cyril
authors.initials: C
authors.lastname: Roblet
authors.forename: Alain
authors.initials: A
authors.lastname: Doyen
authors.forename: Jean
authors.initials: J
authors.lastname: Amiot
authors.forename: Geneviève
authors.initials: G
authors.lastname: Pilon
authors.forename: André
authors.initials: A
authors.lastname: Marette
authors.forename: Laurent
authors.initials: L
authors.lastname: Bazinet
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D001692:Biological Transport; D003956:Dialysis; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D008970:Molecular Weight; D032342:Muscle Cells / Q000201:enzymology / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D015398:Signal Transduction; D013025:Glycine max / Q000737:chemistry*; D014462:Ultrafiltration / Q000295:instrumentation
chemical_list: D010455:Peptides; D055372:AMP-Activated Protein Kinases; D005947:Glucose
keywords: AMPK pathway activation; Electrodialysis with ultrafiltration membranes; Glucose uptake stimulation; Soybean bioactive peptides