title: Mineralization and biocompatibility of Antheraea pernyi (A. pernyi) silk sericin film for potential bone tissue engineering.
abstract: This study aimed to investigate the mineralization of Antheraea pernyi (A. pernyi) silk sericin. Mineralization of A. pernyi sericin was performed by alternative soaking in calcium and phosphate. The inhibition of precipitation of calcium carbonate and von Kossa staining on A. pernyi sericin were tested, and the corresponding results prove that A. pernyi sericin has Ca binding activity. Scanning electron microscope (SEM) observation shows that spherical crystals could be nucleated on the A. pernyi sericin film. These crystals were confirmed to be hydroxyapatite according to FT-IR and XRD spectra, indicating that A. pernyi sericin is capable of mineralization. In addition, cell adhesion and growth activity assay demonstrate that A. pernyi sericin shows excellent biocompatibility for the growth of MG-63 cells.
authors.affiliation: Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, 310058, PRC.
authors.forename: Mingying
authors.initials: M
authors.lastname: Yang
authors.forename: Namita
authors.initials: N
authors.lastname: Mandal
authors.forename: Yajun
authors.initials: Y
authors.lastname: Shuai
authors.forename: Guanshan
authors.initials: G
authors.lastname: Zhou
authors.forename: Sijia
authors.initials: S
authors.lastname: Min
authors.forename: Liangjun
authors.initials: L
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001031:Apatites / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D001842:Bone and Bones; D002118:Calcium / Q000737:chemistry; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018360:Crystallography, X-Ray; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D009036:Moths / Q000737:chemistry*; D047030:Sericins / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D001031:Apatites; D001672:Biocompatible Materials; D008567:Membranes, Artificial; D047030:Sericins; D017886:Durapatite; D002118:Calcium
keywords: Antheraea pernyi (A. pernyi) silk sericin; cell adhesion and growth activity assay; hydroxyapatite; mineralization