title: 6-Hydroxydaidzein enhances adipocyte differentiation and glucose uptake in 3T3-L1 cells.
abstract: Fermented soybean foods have been shown to reduce incidence of diabetes and improve insulin sensitivity. 6-Hydroxydaidzein (6-HD) is a bioactive ingredient isolated from fermented soybean. In this study, we examined the effects of 6-HD on adipocyte differentiation and insulin-stimulated glucose uptake, as well as the mechanisms involved. In our experiments, 6-HD enhanced 3T3-L1 adipocyte differentiation and insulin-stimulated glucose uptake in a dosage-dependent manner. In addition, 6-HD increased peroxisome proliferator-activated receptor gamma (PPARγ) gene expression and PPARγ transcriptional activity. 6-HD increased CCAAT/enhanced binding protein alpha (C/EBPα) expression as well. Although having no effects on glucose transporter type 4 (GLUT4) gene expression, 6-HD facilitated GLUT4 protein translocation to the cell membranes. Our results indicate that 6-HD exhibited the actions of promoting adipocyte differentiation and improving insulin sensitivity by increasing the expression of C/EBPα and facilitating the translocation of GLUT4 via the activation of PPARγ, suggesting that 6-HD can be promising in diabetes management.
authors.affiliation: Pharmacy Department, Xuhui District Central Hospital , 966 Huai Hai M Road, Shanghai 200031, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Qun-Yi
authors.initials: QY
authors.lastname: Li
authors.forename: Xiao-Jin
authors.initials: XJ
authors.lastname: Shi
authors.forename: Shi-Long
authors.initials: SL
authors.lastname: Mao
authors.forename: Yong-Li
authors.initials: YL
authors.lastname: Du
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism*; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007529:Isoflavones / Q000494:pharmacology*; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D045730:Soy Foods / Q000032:analysis* / Q000382:microbiology
chemical_list: D007004:Hypoglycemic Agents; D007529:Isoflavones; D010936:Plant Extracts; D005947:Glucose; C476812:6-hydroxydaidzein