title: Efficient isolation of ion beam-induced mutants for homoeologous loci in common wheat and comparison of the contributions of Glu-1 loci to gluten functionality.
abstract: Ion beam mutations can be efficiently isolated and deployed for functional comparison of homoeologous loci in polyploid plants, and Glu - 1 loci differ substantially in their contribution to wheat gluten functionality. To efficiently conduct genetic analysis, it is beneficial to have multiple types of mutants for the genes under investigation. Here, we demonstrate that ion beam-induced deletion mutants can be efficiently isolated for comparing the function of homoeologous loci of common wheat (Triticum aestivum). Through fragment analysis of PCR products from M2 plants, ion beam mutants lacking homoeologous Glu-A1, Glu-B1 or Glu-D1 loci, which encode high molecular weight glutenin subunits (HMW-GSs) and affect gluten functionality and end-use quality of common wheat, could be isolated simultaneously. Three deletion lines missing Glu-A1, Glu-B1 or Glu-D1 were developed from the original mutants, with the Glu-1 genomic regions deleted in these lines estimated using newly developed DNA markers. Apart from lacking the target HMW-GSs, the three lines all showed decreased accumulation of low molecular weight glutenin subunits (LMW-GSs) and increased amounts of gliadins. Based on the test data of five gluten and glutenin macropolymer (GMP) parameters obtained with grain samples harvested from two environments, we conclude that the genetic effects of Glu-1 loci on gluten functionality can be ranked as Glu-D1 > Glu-B1 > Glu-A1. Furthermore, it is suggested that Glu-1 loci contribute to gluten functionality both directly (by promoting the formation of GMP) and indirectly (through keeping the balance among HMW-GSs, LMW-GSs and gliadins). Finally, the efficient isolation of ion beam mutations for functional comparison of homoeologous loci in polyploid plants and the usefulness of Glu-1 deletion lines for further studying the contribution of Glu-1 loci to gluten functionality are discussed.
authors.affiliation: The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Yushuang
authors.initials: Y
authors.lastname: Yang
authors.forename: Shiming
authors.initials: S
authors.lastname: Li
authors.forename: Kunpu
authors.initials: K
authors.lastname: Zhang
authors.forename: Zhenying
authors.initials: Z
authors.lastname: Dong
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Li
authors.forename: Xueli
authors.initials: X
authors.lastname: An
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Qiufang
authors.initials: Q
authors.lastname: Chen
authors.forename: Zhen
authors.initials: Z
authors.lastname: Jiao
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Huanju
authors.initials: H
authors.lastname: Qin
authors.forename: Daowen
authors.initials: D
authors.lastname: Wang
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D005983:Glutens / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D016133:Polymerase Chain Reaction; D011123:Polyploidy; D014908:Triticum / Q000235:genetics* / Q000378:metabolism
chemical_list: D017931:DNA Primers; D005983:Glutens; C037302:glutenin