title: Analysis of the metabolic properties of maintenance hemodialysis patients with glucose-added dialysis based on high performance liquid chromatography quadrupole time-of-flight mass spectrometry.
abstract: The purpose of this study was to compare the metabolic properties of maintenance hemodialysis patients treated with glucose-containing and glucose-free dialysate using metabonomics. Pre- and post-dialysis serum samples from group G (-) using glucose-free dialysate, and group G (+) using glucose-added dialysate (glucose levels were 5.5 mmol/L) were analyzed and tested with high performance liquid chromatography quadrupole time-of-flight mass spectrometry. Orthogonal signal correction-partial least squares discriminate analysis revealed a significant difference in the post-dialysis metabolic properties between samples from the G (-) and G (+) groups, and concentrations of leucine and dihydroxyprostaglandin F2α were higher in the G (+) group than in the G (-) group. However, markers of reactive lipid mobilization and amino acid release, such as bile acids, aspartate, and valine, were lower in the G (+) group than in the G (-) group. There were no significant differences in excitatory neurotransmitters aspartate and phosphorylated anandamide. Use of liquid chromatography-tandem mass spectrometry metabonomics indicated that using glucose-added dialysate was superior to glucose-free dialysate in the protection of the central nervous system of maintenance hemodialysis patients, but had potential risks in stimulating oxidative stress.
authors.affiliation: Xi'xiang People's Hospital Affiliated to Guangdong Medical College, Shenzhen, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Cui
authors.forename: Yu
authors.initials: Y
authors.lastname: Meng
authors.forename: Dan
authors.initials: D
authors.lastname: Xu
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Feng
authors.forename: Gangyi
authors.initials: G
authors.lastname: Chen
authors.forename: Bo
authors.initials: B
authors.lastname: Hu
authors.forename: Guijuan
authors.initials: G
authors.lastname: Feng
authors.forename: Lianghong
authors.initials: L
authors.lastname: Yin
keywords: glucose-added dialysate; hemodialysis; metabonomics