title: Production of the quinone-methide triterpene maytenin by in vitro adventitious roots of Peritassa campestris (Cambess.) A.C.Sm. (Celastraceae) and rapid detection and identification by APCI-IT-MS/MS.
abstract: Establishment of adventitious root cultures of Peritassa campestris (Celastraceae) was achieved from seed cotyledons cultured in semisolid Woody Plant Medium (WPM) supplemented with 2% sucrose, 0.01% PVP, and 4.0 mg L⁻¹ IBA. Culture period on accumulation of biomass and quinone-methide triterpene maytenin in adventitious root were investigated. The accumulation of maytenin in these roots was compared with its accumulation in the roots of seedlings grown in a greenhouse (one year old). A rapid detection and identification of maytenin by direct injection into an atmospheric-pressure chemical ionization ion trap tandem mass spectrometer (APCI-IT-MS/MS) were performed without prior chromatographic separation. In vitro, the greatest accumulation of biomass occurred within 60 days of culture. The highest level of maytenin--972.11  μ g·g⁻¹ dry weight--was detected at seven days of cultivation; this value was 5.55-fold higher than that found in the roots of seedlings grown in a greenhouse.
authors.affiliation: Núcleo de Bioensaios, Biossíntese e Ecofisiologia de Produtos Naturais, Departamento de Química Orgânica, Instituto de Química, Universidade Estadual Paulista, 14801-970 Araraquara, SP, Brazil.
authors.forename: Tiago Antunes
authors.initials: TA
authors.lastname: Paz
authors.forename: Vânia A F F M
authors.initials: VA
authors.lastname: dos Santos
authors.forename: Marielle Cascaes
authors.initials: MC
authors.lastname: Inácio
authors.forename: Edieidia Souza
authors.initials: ES
authors.lastname: Pina
authors.forename: Ana Maria Soares
authors.initials: AM
authors.lastname: Pereira
authors.forename: Maysa
authors.initials: M
authors.lastname: Furlan
mesh_terms: D019149:Bioreactors; D029753:Celastraceae / Q000254:growth & development*; D003470:Culture Media; D045563:Indolequinones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D036226:Seedlings / Q000254:growth & development; D012639:Seeds / Q000254:growth & development*; D013095:Spermidine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D003470:Culture Media; D045563:Indolequinones; D014315:Triterpenes; C068040:quinone methide; C008335:maytenine; D013095:Spermidine