title: Recombinant lentogenic Newcastle disease virus expressing Ebola virus GP infects cells independently of exogenous trypsin and uses macropinocytosis as the major pathway for cell entry.
abstract: BACKGROUND
  Using reverse genetics, we generated a recombinant low-pathogenic LaSota strain Newcastle disease virus (NDV) expressing the glycoprotein (GP) of Ebola virus (EBOV), designated rLa-EBOVGP, and evaluated its biological characteristic in vivo and in vitro.
  
  
  RESULTS
  The introduction and expression of the EBOV GP gene did not increase the virulence of the NDV vector in poultry or mice. EBOV GP was incorporated into the particle of the vector virus and the recombinant virus rLa-EBOVGP infected cells and spread within them independently of exogenous trypsin. rLa-EBOVGP is more resistant to NDV antiserum than the vector NDV and is moderately sensitive to EBOV GP antiserum. More importantly, infection with rLa-EBOVGP was markedly inhibited by IPA3, indicating that rLa-EBOVGP uses macropinocytosis as the major internalization pathway for cell entry.
  
  
  CONCLUSIONS
  The results demonstrate that EBOV GP in recombinant NDV particles functions independently to mediate the viral infection of the host cells and alters the cell-entry pathway.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Wen
authors.forename: Bolin
authors.initials: B
authors.lastname: Zhao
authors.forename: Kun
authors.initials: K
authors.lastname: Song
authors.forename: Xule
authors.initials: X
authors.lastname: Hu
authors.forename: Weiye
authors.initials: W
authors.lastname: Chen
authors.forename: Dongni
authors.initials: D
authors.lastname: Kong
authors.forename: Jinying
authors.initials: J
authors.lastname: Ge
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, People's Republic of China. zgbu@yahoo.com.
authors.forename: Zhigao
authors.initials: Z
authors.lastname: Bu
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004705:Endocytosis; D051379:Mice; D009522:Newcastle disease virus / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D059386:Reverse Genetics; D016019:Survival Analysis; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D014774:Virulence; D053586:Virus Internalization
chemical_list: D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C113189:envelope glycoprotein, Ebola virus