title: A guide RNA sequence design platform for the CRISPR/Cas9 system for model organism genomes.
abstract: Cas9/CRISPR has been reported to efficiently induce targeted gene disruption and homologous recombination in both prokaryotic and eukaryotic cells. Thus, we developed a Guide RNA Sequence Design Platform for the Cas9/CRISPR silencing system for model organisms. The platform is easy to use for gRNA design with input query sequences. It finds potential targets by PAM and ranks them according to factors including uniqueness, SNP, RNA secondary structure, and AT content. The platform allows users to upload and share their experimental results. In addition, most guide RNA sequences from published papers have been put into our database.
authors.affiliation: Biomedical Engineering Department, College of Engineering, Peking University, Beijing 100871, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Ma
authors.forename: Adam Y
authors.initials: AY
authors.lastname: Ye
authors.forename: Weiguo
authors.initials: W
authors.lastname: Zheng
authors.forename: Lei
authors.initials: L
authors.lastname: Kong
mesh_terms: D000818:Animals; D001483:Base Sequence; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D016678:Genome / Q000235:genetics*; D006801:Humans; D008954:Models, Biological; D058727:RNA, Small Untranslated
chemical_list: D058727:RNA, Small Untranslated