title: A bioactive elastin-like recombinamer reduces unspecific protein adsorption and enhances cell response on titanium surfaces.
abstract: We present the immobilization on synthetic substrates of elastin-like recombinamers (ELR) that combine a bioactive motif for cell adhesion with protein antifouling properties. Physical adsorption of the recombinamers and covalent-grafting through organosilane chemistry were investigated. The biochemically-modified surfaces were thoroughly characterized and tested for protein absorption in serum by fluorescence-labelling, XPS, Ellipsometry, and OWLS. The ELR were successfully grafted and stable, even upon mechanical stresses; being the covalent bonding favourable over physical adsorption. The coated metal surfaces exhibited excellent reduction of serum protein adsorption (9 ng/cm(2)) compared to the bare metal surface (310 ng/cm(2)). Non-specific protein adsorption may mask the introduced bioactive motifs; therefore, the bioactivated surfaces should display serum-protein antifouling properties. Finally, improved hMSCs response was assessed on the bioactivated substrates. In summary, the coatings simultaneously displayed anti-fouling and bioactive properties. These studies investigated key factors to enhance tissue material interactions fundamental for the design of bioactive devices and future biomedical applications.
authors.affiliation: Nanoengineering Research Centre (CRnE), Biomaterials, Biomechanics and Tissue Engineering Group (BiBiTE), Technical University of Catalonia (UPC), Barcelona, Spain. Electronic address: emiliano.salvagni@gmail.com.
authors.forename: Emiliano
authors.initials: E
authors.lastname: Salvagni
authors.affiliation: Nanoengineering Research Centre (CRnE), Biomaterials, Biomechanics and Tissue Engineering Group (BiBiTE), Technical University of Catalonia (UPC), Barcelona, Spain; Department of Bioengineering, University of Washington, Seattle, WA, USA.
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Berguig
authors.affiliation: Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Engel
authors.affiliation: G.I.R. BIOFORGE, CIBER-BBN, Universidad de Valladolid, Valladolid, Spain.
authors.forename: J Carlos
authors.initials: JC
authors.lastname: Rodriguez-Cabello
authors.affiliation: Laboratory for Surface Science and Technology, Swiss Federal Institute of Technology (ETH), Zürich, Switzerland.
authors.forename: Geraldine
authors.initials: G
authors.lastname: Coullerez
authors.affiliation: Laboratory for Surface Science and Technology, Swiss Federal Institute of Technology (ETH), Zürich, Switzerland.
authors.forename: Marcus
authors.initials: M
authors.lastname: Textor
authors.affiliation: Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.
authors.forename: Josep A
authors.initials: JA
authors.lastname: Planell
authors.affiliation: Nanoengineering Research Centre (CRnE), Biomaterials, Biomechanics and Tissue Engineering Group (BiBiTE), Technical University of Catalonia (UPC), Barcelona, Spain.
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Gil
authors.affiliation: Nanoengineering Research Centre (CRnE), Biomaterials, Biomechanics and Tissue Engineering Group (BiBiTE), Technical University of Catalonia (UPC), Barcelona, Spain; MDRCBB-Minnesota Dental Research Center for Biomaterials and Biomechanics, University of Minnesota School of Dentistry, Minneapolis, MN, USA. Electronic address: apari003@umn.edu.
authors.forename: Conrado
authors.initials: C
authors.lastname: Aparicio
mesh_terms: D000327:Adsorption; D000469:Alkaline Phosphatase / Q000378:metabolism; D001565:Benzoates / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D004549:Elastin / Q000494:pharmacology*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D056951:Photoelectron Spectroscopy; D011804:Quinolines / Q000737:chemistry; D011994:Recombinant Proteins / Q000494:pharmacology*; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*
chemical_list: D001565:Benzoates; D020099:Coated Materials, Biocompatible; D011804:Quinolines; D011994:Recombinant Proteins; C069617:3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde; D004549:Elastin; D014025:Titanium; D007770:L-Lactate Dehydrogenase; D000469:Alkaline Phosphatase
keywords: Bioactivity; Biofunctionalization; Biomimetic; Cell response; Protein immobilization; Protein resistance