title: GPX3 from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X-ray analysis.
abstract: The Arabidopsis thaliana glutathione peroxidase 3 (GPX3) gene encodes a glutathione peroxidase with roles in H2O2 homeostasis and signalling. The GPX3 gene sequence was cloned into pGEX-6P1 and overexpressed in Escherichia coli. The GPX3 protein was purified to homogeneity in two chromatographic steps. Various lengths of the GPX3 sequence were used to obtain proteins that yielded crystals using vapour-diffusion techniques, but only GPX3ΔN36 (lacking 36 amino acids from the N-terminus) showed a good diffraction pattern. Its crystals diffracted to 2.8 Å resolution and belonged to space group P65, with unit-cell parameters a = b = 98.241, c = 42.057 Å.
authors.affiliation: Henan Key Laboratory of Plant Stress Biology, Department of Biology, Henan University, Kaifeng 475001, People's Republic of China.
authors.forename: Kun
authors.initials: K
authors.lastname: Li
authors.forename: Qingzhan
authors.initials: Q
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Zhao
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Lou
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D005979:Glutathione Peroxidase / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D017356:Synchrotrons
chemical_list: D029681:Arabidopsis Proteins; C000588782:GPX3 protein, Arabidopsis; D005979:Glutathione Peroxidase
keywords: Arabidopsis thaliana; GPX3; glutathione peroxidase 3