title: Intracerebral injection of oil cyst content of human craniopharyngioma (oil machinery fluid) as a toxic model in the rat brain.
abstract: Craniopharyngiomas (CPs) are benign epithelial cystic tumors of the sellar and suprasellar region with a high survival rate and high recurrence in children. CPs contain dense oily fluid, but little is known yet about this content and its contribution to tissue damage and tumoral growth. In this study, we developed a simple experimental model produced by intracortical injection to rats of the cyst fluid content collected from human CPs to explore its possible contribution to brain tissue damage. The cyst fluid of the CPs ("oil machinery fluid") was collected during surgical removal, briefly preserved and further tested in rats through intracortical infusion. The group receiving "oil machinery fluid" presented increased reactive oxygen species formation, oxidative damage to lipids and reactive gliosis accompanied by augmented immunoreactivity to peroxiredoxin and thioredoxin reductase 1 at 15, 30 and 45 days post-injection. Other markers of inflammation and cell damage were stimulated at all post-lesion days tested. There was also a body weight gain. The persistence of tissue damage and oxidative stress suggests that "oil machinery fluid" exerts progressive alterations similar to those observed in patients with CPs, supporting the concept that some components of cyst fluid may contribute to brain tissue damage in these patients.
authors.affiliation: Sección de Estudios de Posgrado e Investigación de la Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico City, Mexico; Departamento de Neuropatología, Instituto Nacional de Neurología y Neurocirugía. Mexico City, Mexico.
authors.forename: Martha Lilia
authors.initials: ML
authors.lastname: Tena-Suck
authors.affiliation: Sección de Estudios de Posgrado e Investigación de la Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico City, Mexico.
authors.forename: Ma Elena
authors.initials: ME
authors.lastname: Hernández-Campos
authors.affiliation: Laboratorio de Neuropatología Experimental, Instituto Nacional de Neurología y Neurocirugía, Mexico City, Mexico.
authors.forename: Alma
authors.initials: A
authors.lastname: Ortiz-Plata
authors.affiliation: Departamento de Neuropatología, Instituto Nacional de Neurología y Neurocirugía. Mexico City, Mexico.
authors.forename: Citlaltepetl
authors.initials: C
authors.lastname: Salinas-Lara
authors.affiliation: Laboratorio de Aminoácidos Excitadores, Instituto Nacional de Neurología y Neurocirugía, Mexico City, Mexico.
authors.forename: Ana Laura
authors.initials: AL
authors.lastname: Colín-González
authors.affiliation: Laboratorio de Aminoácidos Excitadores, Instituto Nacional de Neurología y Neurocirugía, Mexico City, Mexico. Electronic address: absada@yahoo.com.
authors.forename: Abel
authors.initials: A
authors.lastname: Santamaría
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism / Q000473:pathology; D003397:Craniopharyngioma / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005911:Gliosis / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D018384:Oxidative Stress; D054464:Peroxiredoxins / Q000378:metabolism; D010911:Pituitary Neoplasms / Q000378:metabolism* / Q000473:pathology; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D054481:Thioredoxin Reductase 1 / Q000378:metabolism; D014020:Tissue Extracts / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015430:Weight Gain; D055815:Young Adult
chemical_list: D051267:NF-E2-Related Factor 2; D016328:NF-kappa B; C495637:Nfe2l2 protein, rat; D017382:Reactive Oxygen Species; D014020:Tissue Extracts; D014409:Tumor Necrosis Factor-alpha; D054464:Peroxiredoxins; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat; D054481:Thioredoxin Reductase 1; C516126:Txnrd1 protein, rat
keywords: Craniopharyngioma; Experimental toxic model; Oil machinery fluid; Oxidative damage; Reactive gliosis