title: Biological production of 2-butanone in Escherichia coli.
abstract: An Escherichia coli (E. coli) strain was engineered to synthesize 2-butanone from glucose by extending the 2,3-butanediol synthesis reaction sequence catalyzed by exogenous enzymes. To convert 2,3-butanediol to 2-butanone, B12-dependent glycerol dehydratase from Klebsiella pneumoniae was introduced into E. coli. It has been proposed that the enzyme has a weak activity toward 2,3-butanediol. The activity in E. coli is confirmed in this study. Furthermore, co-expressing coenzyme B12 reactivators increased the 2-butanone titer. This demonstration of 2-butanone production by extending the 2,3-butanediol biosynthetic pathway provides the possibility to produce this valuable chemical renewably.
authors.affiliation: Department of Chemistry, University of California, Davis, One Shields Avenue, Davis, CA 95616 (USA), Fax: (+1) 530-752-8995; Central R&D laboratories, New Business Development, Asahi Kasei Corporation, 2-1 Samejima, Fuji, Shizuoka 416-8501 (Japan).
authors.forename: Hisanari
authors.initials: H
authors.lastname: Yoneda
authors.forename: Dean J
authors.initials: DJ
authors.lastname: Tantillo
authors.forename: Shota
authors.initials: S
authors.lastname: Atsumi
mesh_terms: D002074:Butanones / Q000378:metabolism*; D002072:Butylene Glycols / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006836:Hydro-Lyases / Q000235:genetics / Q000378:metabolism
chemical_list: D002074:Butanones; D002072:Butylene Glycols; C026978:2,3-butylene glycol; C005222:methylethyl ketone; D006836:Hydro-Lyases; C020671:glycerol dehydratase; D005947:Glucose
keywords: biosynthesis; enzymes; renewable resources; sustainable chemistry; synthetic biology