title: Origins and functional specialization of macrophages and of conventional and monocyte-derived dendritic cells in mouse skin.
abstract: In the skin, the lack of markers permitting the unambiguous identification of macrophages and of conventional and monocyte-derived dendritic cells (DCs) complicates understanding of their contribution to skin integrity and to immune responses. By combining CD64 and CCR2 staining, we successfully identified each of these cell types and studied their origin, transcriptomic signatures, and migratory and T cell stimulatory properties. We also analyzed the impact of microbiota on their development and their contribution to skin inflammation during contact hypersensitivity. Dermal macrophages had a unique scavenging role and were unable to migrate and activate T cells. Conventional dermal DCs excelled both at migrating and activating T cells. In the steady-state dermis, monocyte-derived DCs are continuously generated by extravasated Ly-6C(hi) monocytes. Their T cell stimulatory capacity combined with their poor migratory ability made them particularly suited to activate skin-tropic T cells. Therefore, a high degree of functional specialization occurs among the mononuclear phagocytes of the skin.
authors.affiliation: Centre d'Immunologie de Marseille-Luminy (CIML), UM2 Aix-Marseille Université, 13288 Marseille Cedex 9, France; INSERM U1104, 13288 Marseille Cedex 9, France; CNRS UMR7280, 13288 Marseille Cedex 9, France.
authors.forename: Samira
authors.initials: S
authors.lastname: Tamoutounour
authors.forename: Martin
authors.initials: M
authors.lastname: Guilliams
authors.forename: Frédéric
authors.initials: F
authors.lastname: Montanana Sanchis
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Dorothea
authors.initials: D
authors.lastname: Terhorst
authors.forename: Camille
authors.initials: C
authors.lastname: Malosse
authors.forename: Emeline
authors.initials: E
authors.lastname: Pollet
authors.forename: Laurence
authors.initials: L
authors.lastname: Ardouin
authors.forename: Hervé
authors.initials: H
authors.lastname: Luche
authors.forename: Cindy
authors.initials: C
authors.lastname: Sanchez
authors.forename: Marc
authors.initials: M
authors.lastname: Dalod
authors.forename: Bernard
authors.initials: B
authors.lastname: Malissen
authors.forename: Sandrine
authors.initials: S
authors.lastname: Henri
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D039481:CD11b Antigen / Q000032:analysis; D019070:Cell Lineage; D002634:Chemotaxis, Leukocyte; D002850:Chromatography, Gel; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D003877:Dermatitis, Contact / Q000276:immunology / Q000473:pathology; D020405:Dermis / Q000166:cytology; D018507:Gene Expression Regulation, Developmental; D016130:Immunophenotyping / Q000379:methods; D007801:Langerhans Cells / Q000166:cytology / Q000276:immunology; D008211:Lymphocyte Cooperation; D008264:Macrophages / Q000166:cytology* / Q000502:physiology; D051379:Mice; D064307:Microbiota / Q000276:immunology; D009000:Monocytes / Q000166:cytology; D025341:Principal Component Analysis; D011828:Radiation Chimera; D054390:Receptors, CCR2 / Q000032:analysis; D017452:Receptors, IgG / Q000032:analysis; D012867:Skin / Q000166:cytology* / Q000276:immunology / Q000382:microbiology; D013047:Specific Pathogen-Free Organisms; D013194:Staining and Labeling / Q000379:methods; D059467:Transcriptome
chemical_list: D000943:Antigens, Differentiation; D039481:CD11b Antigen; C515335:Ccr2 protein, mouse; D054390:Receptors, CCR2; D017452:Receptors, IgG