title: Effect of Nd:YAG laser-nitriding-treated titanium nitride surface over Ti6Al4V substrate on the activity of MC3T3-E1 cells.
abstract: Ti6Al4V discs with a thickness of 2.5 mm and dimensions of 15 × 15 mm2 were fabricated. The titanium nitride (TiN) surface was formed via Nd:YAG laser-nitriding. A sandblast acid-etched (SA) surface was used as a control. Scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), and surface roughness tests were conducted to study the surface and cross-section morphologies as well as the properties of TiN and SA surfaces. MC3T3-E1 osteoblast-like cells were cultured on the TiN and SA surfaces to evaluate the effect of TiN surface on cellular behaviors, including attachment, proliferation and differentiation. Morphological testing results revealed that the cross-section of TiN exhibited dendritic crystallization without cracking. The proliferation and differentiation of MC3T3-E1 cells on the laser-nitriding TiN surface were significantly increased compared to those cultured on SA surface. These findings suggested that the TiN surface generated from Nd:YAG laser-nitriding were favorable for the proliferation and differentiation of MC3T3-E1 cells, which is significant for implant surface modification.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, Wuhan University, Wuhan, China Department of Oral Implantology, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Yingyuan
authors.initials: Y
authors.lastname: Ning
authors.forename: Haixiao
authors.initials: H
authors.lastname: Zou
authors.forename: Si
authors.initials: S
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Bai
authors.forename: Aihua
authors.initials: A
authors.lastname: Wang
authors.forename: Haibin
authors.initials: H
authors.lastname: Xia
mesh_terms: D041721:3T3-L1 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000497:Alloys; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001842:Bone and Bones / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D053844:Lasers, Solid-State; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009354:Neodymium / Q000737:chemistry; D010006:Osteoblasts / Q000166:cytology; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; C031462:titanium alloy (TiAl6V4); D009354:Neodymium; C041500:titanium nitride; D014025:Titanium; D000469:Alkaline Phosphatase
keywords: MC3T3-E1 cells; TiN surface; adhesion; differentiation; laser-nitriding