title: [Circular dichroism chromatography of secondary structures of anti-HBV IgY in eggs from hens immunized with recombinant yeast HBV vaccine].
abstract: OBJECTIVE
  To study the generation and structure of anti-HBV IgY in eggs from hens immunized with recombinant yeast HBV vaccine.
  
  
  METHODS
  From the hens immunized three times with the recombinant yeast HBV vaccine, the eggs were collected to extract anti-HBV IgY. After purified, the anti- HBV IgY was detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and reverse phase high-performance liquid chromatography (RP-HPLC), and its secondary structure was examined by circular dichroism (CD) chromatography.
  
  
  RESULTS
  The relative molecular mass (Mr;) of anti-HBV IgY expressed by the recombinant yeast HBV vaccine on hens was 178 000. CD chromatography showed that the α-helix, β-fold, β-turn and random coil of anti-HBV IgY had 38, 0, 44 and 17 amino acids, respectively.
  
  
  CONCLUSIONS
  The anti-HBV IgY can be expressed in eggs from hens immunized with recombinant yeast HBV vaccine. The α-helix and β-turn are major secondary structures of anti-HBV IgY.
authors.affiliation: Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China.
authors.forename: Xixiu
authors.initials: X
authors.lastname: Fang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Fang
authors.forename: Dongmei
authors.initials: D
authors.lastname: Wang
authors.forename: Fugang
authors.initials: F
authors.lastname: Qi
mesh_terms: D000818:Animals; D002645:Chickens / Q000276:immunology*; D002942:Circular Dichroism; D004274:DNA, Recombinant / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D006515:Hepatitis B virus / Q000276:immunology*; D007114:Immunization; D007136:Immunoglobulins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D010063:Ovum / Q000276:immunology*; D017433:Protein Structure, Secondary; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
chemical_list: D004274:DNA, Recombinant; C016512:IgY; D007136:Immunoglobulins; D014765:Viral Vaccines