title: Unsaponifiable fraction from extra virgin olive oil inhibits the inflammatory response in LPS-activated murine macrophages.
abstract: Extra virgin olive oil (EVOO) has demonstrated great anti-inflammatory properties. Nowadays, it is clear that its minor components have a key role in these beneficial effects. However, the contribution of the unsaponifiable fraction (UF) to these healthy effects remains unknown. The present study was designed to evaluate UF in LPS stimulated peritoneal macrophages isolated from mice. NO production was analysed by the Griess method and intracellular ROS by fluorescence analysis. In addition, MAPK family activation, IKBα degradation, NFκB-p65, iNOS and COX-2 expression were determined by Western blot. UF exerted anti-inflammatory and anti-oxidant effects inhibiting LPS-induced intracellular ROS and nitrite production. Additionally, UF decreased COX-2 and iNOS protein expression. These effects were related with a down-regulation in NFκB signal signalling pathways and in MAPK phosphorylation. UF of EVOO compounds could play an important role in the anti-inflammatory effect of virgin olive oils and probably provide an attractive complement in management of inflammatory diseases.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Seville, Profesor García González Street 2, 41012 Seville, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Cardeno
authors.forename: Marina
authors.initials: M
authors.lastname: Sanchez-Hidalgo
authors.forename: Marina
authors.initials: M
authors.lastname: Aparicio-Soto
authors.forename: Catalina
authors.initials: C
authors.lastname: Alarcón-de-la-Lastra
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000276:immunology; D015870:Gene Expression / Q000187:drug effects; D008070:Lipopolysaccharides / Q000009:adverse effects / Q000276:immunology; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology*; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D016328:NF-kappa B; D000069463:Olive Oil; D010938:Plant Oils; D051546:Cyclooxygenase 2
keywords: Extra virgin olive oil; LPS-stimulated macrophages; MAPK; NFκB; ROS; Unsaponifiable fraction