title: Protective effect of dieckol isolated from Ecklonia cava against ethanol caused damage in vitro and in zebrafish model.
abstract: In the present study, the protective effects of phlorotannins isolated from Ecklonia cava against ethanol-induced cell damage and apoptosis were investigated both in vitro and in vivo. Three phlorotannin compounds, namely phloroglucinol, eckol and dieckol, were successively isolated and identified from the extract. Dieckol showed the strongest protective effect against ethanol-induced cell apoptosis in Chang liver cells, with the lowest cytotoxicity. It was observed that dieckol reduced cell apoptosis through activation of Bcl-xL and PARP, and down-regulation of Bax and caspase-3 in Western blot analyses. In the in vivo study, the protective effect of ethanol induced by dieckol was investigated in a zebrafish model. The dieckol treated group scavenged intracellural reactive oxygen species and prevented lipid peroxidation and ethanol induced cell death in the zebrafish embryo. In conclusion, dieckol isolated from E. cava might possess a potential protective effect against ethanol-induced liver diseases.
authors.affiliation: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Min-Cheol
authors.initials: MC
authors.lastname: Kang
authors.forename: Kil-Nam
authors.initials: KN
authors.lastname: Kim
authors.forename: Sung-Myung
authors.initials: SM
authors.lastname: Kang
authors.forename: Xiudong
authors.initials: X
authors.lastname: Yang
authors.forename: Eun-A
authors.initials: EA
authors.lastname: Kim
authors.forename: Choon Bok
authors.initials: CB
authors.lastname: Song
authors.forename: Jae-Woon
authors.initials: JW
authors.lastname: Nah
authors.forename: Mi-Kyeong
authors.initials: MK
authors.lastname: Jang
authors.forename: Jung-Suck
authors.initials: JS
authors.lastname: Lee
authors.forename: Won-Kyo
authors.initials: WK
authors.lastname: Jung
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Jeon
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001562:Benzimidazoles; D001572:Benzofurans / Q000302:isolation & purification / Q000494:pharmacology*; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002492:Central Nervous System Depressants / Q000633:toxicity*; D002851:Chromatography, High Pressure Liquid; D004625:Embryo, Nonmammalian / Q000473:pathology; D000431:Ethanol / Q000633:toxicity*; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D000459:Phaeophyceae / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012624:Seaweed / Q000737:chemistry*; D015027:Zebrafish / Q000502:physiology*
chemical_list: D051017:Apoptosis Regulatory Proteins; D001562:Benzimidazoles; D001572:Benzofurans; D002492:Central Nervous System Depressants; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; C503840:dieckol; D000431:Ethanol; D007770:L-Lactate Dehydrogenase; C017807:bisbenzimide ethoxide trihydrochloride
keywords: Alcohol; Dieckol; Ecklonia cava; Hepato-protective effect; Phlorotannin; Zebrafish