title: Proteoglycans from the vertebral cartilage of the clearnose skate, Raja eglanteria: Inhibition of hydroxyapatite formation.
abstract: Studies were undertaken to examine the importance of organic proteoglycan matrices in the calcification of elasmobranch vertebral cartilage. Proteoglycans were extracted from the vertebral cartilage of the clearnose skate, Raja eglanteria Bosc, with a 3M guanidine hydrochloride/10% EDTA solution. Proteoglycan solutions (12 μg ml(-1)) were effective inhibitors of hydroxyapatite formation in vitro from high concentration calcium phosphate solutions. Inhibition of crystal formation appears to occur through the restriction of phase transformation from a calcium phosphate precursor to hydroxyapatite crystals. The concentration and/or degradation of proteoglycans in elasmobranch vertebral cartilage may have a physiological role in the production of alternating mineral-rich and mineral-poor growth zones, currently used in ageing studies.
authors.affiliation: The College of William and Mary, School of Marine Science, Virginia Institute of Marine Science, Gloucester Point, VA, 23062, USA.
authors.forename: J J
authors.initials: JJ
authors.lastname: Gelsleichter
authors.forename: J A
authors.initials: JA
authors.lastname: Musick
authors.forename: P
authors.initials: P
authors.lastname: Van Veld