title: Rapid conversion of EUCOMM/KOMP-CSD alleles in mouse embryos using a cell-permeable Cre recombinase.
abstract: We describe here use of a cell-permeable Cre to efficiently convert the EUCOMM/KOMP-CSD tm1a allele to the tm1b form in preimplantation mouse embryos in a high-throughput manner, consistent with the requirements of the International Mouse Phenotyping Consortium-affiliated NIH KOMP2 project. This method results in rapid allele conversion and minimizes the use of experimental animals when compared to conventional Cre transgenic mouse breeding, resulting in a significant reduction in costs and time with increased welfare benefits.
authors.affiliation: The Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, CB10 1SA, UK, er1@sanger.ac.uk.
authors.forename: Edward
authors.initials: E
authors.lastname: Ryder
authors.forename: Brendan
authors.initials: B
authors.lastname: Doe
authors.forename: Diane
authors.initials: D
authors.lastname: Gleeson
authors.forename: Richard
authors.initials: R
authors.lastname: Houghton
authors.forename: Priya
authors.initials: P
authors.lastname: Dalvi
authors.forename: Evelyn
authors.initials: E
authors.lastname: Grau
authors.forename: Bishoy
authors.initials: B
authors.lastname: Habib
authors.forename: Evelina
authors.initials: E
authors.lastname: Miklejewska
authors.forename: Stuart
authors.initials: S
authors.lastname: Newman
authors.forename: Debarati
authors.initials: D
authors.lastname: Sethi
authors.forename: Caroline
authors.initials: C
authors.lastname: Sinclair
authors.forename: Sapna
authors.initials: S
authors.lastname: Vyas
authors.forename: Hannah
authors.initials: H
authors.lastname: Wardle-Jones
authors.forename: Joanna
authors.initials: J
authors.lastname: Bottomley
authors.forename: James
authors.initials: J
authors.lastname: Bussell
authors.forename: Antonella
authors.initials: A
authors.lastname: Galli
authors.forename: Jennifer
authors.initials: J
authors.lastname: Salisbury
authors.forename: Ramiro
authors.initials: R
authors.lastname: Ramirez-Solis
mesh_terms: D000483:Alleles; D000818:Animals; D001947:Breeding; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000235:genetics*; D053595:Embryonic Stem Cells; D019426:Integrases / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic
chemical_list: C045073:Cre recombinase; D019426:Integrases