title: Effect of peritoneal cavity lavage with 0.9% and 3.0% saline solution in the lung and spleen of gerbils with induced peritonitis.
abstract: BACKGROUND
  Peritoneal cavity lavage is used widely in the treatment of peritonitis. Nonetheless, some studies question its rationale and prove it to be deleterious to the mesothelium. The present study aims to determine whether 0.9% and 3.0% saline lavage of the peritoneal cavity have an effect on the early systemic inflammatory response, namely, in the lung injury and splenic cellularity of gerbils with induced peritonitis.
  
  
  METHODS
  Thirty-four male gerbils were divided into four groups: Control (n=9), submitted to laparotomy at time zero, re-laparotomy after 2 h, and sacrificed after a total of 6 h from start; untreated (n=8), submitted to peritonitis induction through cecal ligation and puncture (CLP) at time zero, re-laparotomy intended for drying of abdominal cavity and resection of the ischemic cecum after 2 h, and sacrifice after a total of 6 h from start; saline (n=8), submitted to peritonitis induction through CLP at time zero, re-laparotomy intended for warm 0.9% saline lavage of the abdominal cavity and resection of the ischemic cecum after 2 h, and sacrificed after a total of 6 h from start; and hypertonic (n=9), submitted to peritonitis induction through CLP at time zero, re-laparotomy intended for warm hypertonic saline (3.0%) lavage of the abdominal cavity and resection of the ischemic cecum after 2 h, and sacrificed after a total of 6 h from start. After sacrifice, we collected the left lung and the spleen for morphometric analysis.
  
  
  RESULTS
  In the both the saline and hypertonic groups, there was significant decrease in the mean nuclei count in the lungs, compared with the untreated group (p<0.01). There was no difference in terms of nuclei count in the spleen among groups (p>0.05).
  
  
  CONCLUSIONS
  The present study demonstrated that the peritoneal lavage with large volumes of warm 0.9% and 3.0% saline has a beneficial effect on the early systemic inflammatory response in infected animals, modulating and reducing the lung injury but having no effect on splenic cell count.
authors.affiliation: 1 Postgraduate Program in Surgical Sciences, Universidade Federal de Minas Gerais , Belo Horizonte, Brazil .
authors.forename: Vinícius Rodrigues Taranto
authors.initials: VR
authors.lastname: Nunes
authors.forename: Rafael Calvão
authors.initials: RC
authors.lastname: Barbuto
authors.forename: Paula Vieira Teixeira
authors.initials: PV
authors.lastname: Vidigal
authors.forename: Guilherme Nogueira
authors.initials: GN
authors.lastname: Pena
authors.forename: Silvia Lunardi
authors.initials: SL
authors.lastname: Rocha
authors.forename: Lucas Tourinho
authors.initials: LT
authors.lastname: de Siqueira
authors.forename: Marcelo Vidigal
authors.initials: MV
authors.lastname: Caliari
authors.forename: Ivana Duval
authors.initials: ID
authors.lastname: de Araujo
mesh_terms: D000818:Animals; D005849:Gerbillinae; D007813:Laparotomy; D008168:Lung / Q000473:pathology*; D055370:Lung Injury / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D010529:Peritoneal Cavity; D010538:Peritonitis / Q000473:pathology / Q000628:therapy*; D012965:Sodium Chloride / Q000008:administration & dosage*; D013154:Spleen / Q000473:pathology*; D007507:Therapeutic Irrigation / Q000379:methods*
chemical_list: D012965:Sodium Chloride