title: Antiproliferative activity of Cyanophora paradoxa pigments in melanoma, breast and lung cancer cells.
abstract: The glaucophyte Cyanophora paradoxa (Cp) was chemically investigated to identify pigments efficiently inhibiting malignant melanoma, mammary carcinoma and lung adenocarcinoma cells growth. Cp water and ethanol extracts significantly inhibited the growth of the three cancer cell lines in vitro, at 100 µg · mL(-1). Flash chromatography of the Cp ethanol extract, devoid of c-phycocyanin and allophycocyanin, enabled the collection of eight fractions, four of which strongly inhibited cancer cells growth at 100 µg · mL(-1). Particularly, two fractions inhibited more than 90% of the melanoma cells growth, one inducing apoptosis in the three cancer cells lines. The detailed analysis of Cp pigment composition resulted in the discrimination of 17 molecules, ten of which were unequivocally identified by high resolution mass spectrometry. Pheophorbide a, β-cryptoxanthin and zeaxanthin were the three main pigments or derivatives responsible for the strong cytotoxicity of Cp fractions in cancer cells. These data point to Cyanophora paradoxa as a new microalgal source to purify potent anticancer pigments, and demonstrate for the first time the strong antiproliferative activity of zeaxanthin and β-cryptoxanthin in melanoma cells.
authors.affiliation: University of La Rochelle, UMRi CNRS 7266 LIENSs, F-17042, La Rochelle, France. lpicot@univ-lr.fr.
authors.forename: Paul-Hubert
authors.initials: PH
authors.lastname: Baudelet
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Gagez
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Bérard
authors.forename: Camille
authors.initials: C
authors.lastname: Juin
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bridiau
authors.forename: Raymond
authors.initials: R
authors.lastname: Kaas
authors.forename: Valérie
authors.initials: V
authors.lastname: Thiéry
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Cadoret
authors.forename: Laurent
authors.initials: L
authors.lastname: Picot
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D065366:Cryptoxanthins; D044424:Cyanophora / Q000737:chemistry* / Q000378:metabolism; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy*; D061986:MCF-7 Cells; D008545:Melanoma / Q000188:drug therapy*; D010860:Pigments, Biological / Q000737:chemistry / Q000494:pharmacology*; D012878:Skin Neoplasms; D024341:Xanthophylls / Q000737:chemistry / Q000494:pharmacology; D065146:Zeaxanthins; D000096142:Cutaneous Malignant Melanoma
chemical_list: D000970:Antineoplastic Agents; D065366:Cryptoxanthins; D010860:Pigments, Biological; D024341:Xanthophylls; D065146:Zeaxanthins