title: In vitro somatic embryogenesis from callus culture of the timber yielding treeHardwickia binata Roxb.
abstract: Somatic embryogenesis was achieved in callus cultures derived from immature cotyledonary explants ofHardwickia binata Roxb., a multipurpose leguminous tree, on semisolid modified Murashige and Skoog's (mMS) medium containing 2900 mg/l potassium nitrate (KNO3) supplemented with 4.64 µM kinetin (Kn) and 5.37µM a-naphthaleneacetic acid (NAA). Somatic embryos proliferated rapidly after transfer to MS basal medium supplemented with 2052.6 µM L-glutamine and 0.084 µM gibberellic acid (GA3). Maturation of somatic embryos was achieved on half-strength MS basal medium supplemented with 1.23 µM IBA and 2% (w/v) sucrose. Histological studies confirmed different developmental stages of somatic embryogenesis inHardwickia binata.
authors.affiliation: Plant Tissue Culture Laboratory, Regional Plant Resource Centre, 751 015, Bhubaneswar, India.
authors.forename: A B
authors.initials: AB
authors.lastname: Das
authors.forename: G R
authors.initials: GR
authors.lastname: Rout
authors.forename: P
authors.initials: P
authors.lastname: Das