title: 1-hydroxypyrene levels in blood samples of rats after exposure to generator fumes.
abstract: Polynuclear Aromatic Hydrocarbons (PAHs) are a major component of fuel generator fumes. Carcinogenicity of these compounds has long been established. In this study, 37 Swiss albino rats were exposed to generator fumes at varied distances for 8 hours per day for a period of 42 days and the level of 1-hydroxypyrene in their blood was evaluated. This study also tried to correlate the level of blood 1-hyroxypyrene with the distance from the source of pollution. Plasma was collected by centrifuging the whole blood sample followed by complete hydrolysis of the conjugated 1-hydroxypyrene glucuronide to yield the analyte of interest, 1-hydroxypyrene, which was achieved using beta glucuronidase. High performance liquid chromatography (HPLC) with UV detector was used to determine the 1-hydroxypyrene concentrations in the blood samples. The mobile phase was water:methanol (12:88 v/v) isocratic run at the flow rate of 1.2 mL/min with CI8 stationary phase at 250 nm. After 42 days of exposure, blood concentration level of 1-hydroxypyrene ranged from 34 μg/mL to 26.29 μg/mL depending on the distance from source of exposure. The control group had no 1-hydroxypyrene in their blood. After the period of exposure, percentage of death correlated with the distance from the source of exposure. Percentage of death ranged from 56% to zero depending on the proximity to source of pollution.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Lagos.
authors.forename: Clinton
authors.initials: C
authors.lastname: Ifegwu
authors.forename: Miriam N
authors.initials: MN
authors.lastname: Igwo-Ezikpe
authors.forename: Chimezie
authors.initials: C
authors.lastname: Anyakora
authors.forename: Akinniyi
authors.initials: A
authors.lastname: Osuntoki
authors.forename: Kafayat A
authors.initials: KA
authors.lastname: Oseni
authors.forename: Eragbae O
authors.initials: EO
authors.lastname: Alao
keywords: 1-hydroxypyrene; HPLC; cancer; generator fumes; rats