title: Alteration of bacterial adhesion induced by the substrate stiffness.
abstract: The aim of this paper is to study the impact of the substrate stiffness on the bacterial adhesion. For this purpose, agarose hydrogels are used as substrates with controlled mechanical properties. Indeed, the elastic modulus of these hydrogels, more precisely the shear storage moduli G', evolves with the agarose concentration (in this study from 0.75% to 3%). Other physico-chemical characteristics of the surface, known to be involved in bacterial adhesion, as hydrophobicity, were confirmed to remain constant. Two marine bacterial strains, a positive Gram Bacillus sp. 4J6 and a negative Gram Pseudoalteromonas sp. D41 were selected. Their retention on the substrates was analysed by confocal laser scanning microscopy and by counting of viable adhered bacteria. It was demonstrated that surface elastic modulus correlated with bacterial retention. Bacteria D41 adhered in higher numbers to rigid surfaces. For 4J6, bacterial adhesion patterns were changed: clusterings were observed on surfaces with lower elastic modulus. Furthermore, a proteomic study, based on the total soluble proteome of D41 strain, highlights an impact of elastic modulus on proteins synthesis. These data demonstrated an adapted response of adhering bacteria on hydrogels of varying mechanical properties.
authors.affiliation: Université de Bretagne-Sud, EA3884, LBCM, IUEM, F-56100 Lorient, France. Electronic address: guegancaroline@yahoo.fr.
authors.forename: C
authors.initials: C
authors.lastname: Guégan
authors.affiliation: Université de Bretagne-Sud, EA3884, LBCM, IUEM, F-56100 Lorient, France.
authors.forename: J
authors.initials: J
authors.lastname: Garderes
authors.affiliation: Université de Bretagne-Sud, EA3884, LBCM, IUEM, F-56100 Lorient, France.
authors.forename: Gaël
authors.initials: G
authors.lastname: Le Pennec
authors.affiliation: Station biologique de Roscoff, Plateforme Metabomer FR2424, Place Georges Teissier, 29680 Roscoff, France.
authors.forename: F
authors.initials: F
authors.lastname: Gaillard
authors.affiliation: Université de Bretagne-Sud, EA3884, LBCM, IUEM, F-56100 Lorient, France.
authors.forename: F
authors.initials: F
authors.lastname: Fay
authors.affiliation: Université de Bretagne-Sud, EA3884, LBCM, IUEM, F-56100 Lorient, France.
authors.forename: I
authors.initials: I
authors.lastname: Linossier
authors.affiliation: INRA, AgroParisTech, UMR1319 Micalis, Equipe Bioadhésion Biofilm et Hygiène des Matériaux, 25 Avenue de la République, 91300 Massy, France.
authors.forename: J-M
authors.initials: JM
authors.lastname: Herry
authors.affiliation: AgroParisTech, INRA, UMR Micalis Equipe Bioadhésion Biofilm et Hygiène des Matériaux, 25 Avenue de la République, 91300 Massy, France.
authors.forename: M-N Bellon
authors.initials: MN
authors.lastname: Fontaine
authors.affiliation: Université de Bretagne-Sud, EA3884, LBCM, IUEM, F-56100 Lorient, France. Electronic address: Karine.rehel@univ-ubs.fr.
authors.forename: K Vallée
authors.initials: KV
authors.lastname: Réhel
mesh_terms: D001407:Bacillus / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D015169:Colony Count, Microbial; D055119:Elastic Modulus / Q000187:drug effects; D004583:Electrons; D020100:Hydrogels / Q000737:chemistry* / Q000494:pharmacology*; D013058:Mass Spectrometry; D050296:Microbial Viability / Q000187:drug effects; D009068:Movement / Q000187:drug effects; D040901:Proteomics; D031161:Pseudoalteromonas / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D012995:Solubility / Q000187:drug effects; D013500:Surface Tension / Q000187:drug effects
chemical_list: D001426:Bacterial Proteins; D020100:Hydrogels
keywords: Adhesion; Agarose hydrogel; Bacteria; Biofilm; Stiffness substrate