title: HEK293 cell culture media study towards bioprocess optimization: Animal derived component free and animal derived component containing platforms.
abstract: The increasing demand for biopharmaceuticals produced in mammalian cells has lead industries to enhance bioprocess volumetric productivity through different strategies. Among those strategies, cell culture media development is of major interest. In the present work, several commercially available culture media for Human Embryonic Kidney cells (HEK293) were evaluated in terms of maximal specific growth rate and maximal viable cell concentration supported. The main objective was to provide different cell culture platforms which are suitable for a wide range of applications depending on the type and the final use of the product obtained. Performing simple media supplementations with and without animal derived components, an enhancement of cell concentration from 2 × 10(6) cell/mL to 17 × 10(6) cell/mL was achieved in batch mode operation. Additionally, the media were evaluated for adenovirus production as a specific application case of HEK293 cells. None of the supplements interfered significantly with the adenovirus infection although some differences were encountered in viral productivity. To the best of our knowledge, the high cell density achieved in the work presented has never been reported before in HEK293 batch cell cultures and thus, our results are greatly promising to further study cell culture strategies in bioreactor towards bioprocess optimization.
authors.affiliation: Chemical Engineering Department, Universitat Autònoma de Barcelona, Campus Bellaterra, Cerdanyola del Vallès, 08193 Barcelona, Spain. Electronic address: Leticia.Liste@uab.cat.
authors.forename: Leticia
authors.initials: L
authors.lastname: Liste-Calleja
authors.affiliation: Chemical Engineering Department, Universitat Autònoma de Barcelona, Campus Bellaterra, Cerdanyola del Vallès, 08193 Barcelona, Spain.
authors.forename: Martí
authors.initials: M
authors.lastname: Lecina
authors.affiliation: Chemical Engineering Department, Universitat Autònoma de Barcelona, Campus Bellaterra, Cerdanyola del Vallès, 08193 Barcelona, Spain.
authors.forename: Jordi Joan
authors.initials: JJ
authors.lastname: Cairó
mesh_terms: D000256:Adenoviridae / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D000818:Animals; D061249:Batch Cell Culture Techniques / Q000379:methods; D019149:Bioreactors / Q000592:standards*; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology*; D057809:HEK293 Cells / Q000166:cytology* / Q000187:drug effects* / Q000821:virology; D006801:Humans
chemical_list: D003470:Culture Media
keywords: Cell culture media; HEK293; High cell density culture; Recombinant adenovirus; Suspension cell culture