title: Application of high-speed counter-current chromatography for isolation of triterpenes from Schisandra Chinensis (Turcz.) Baill and induction apoptosis mechanism of HSC-T6.
abstract: Triterpenes have shown many beneficial activities in researches, but their separation and preparation usually require multiple methods. Following an initial cleaning-up step on the AB-8 macroporous resin, a preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system comprising chloroform-n-butyl alcohol-methanol-water (10:1:7:4, v/v/v/v) was used to isolate and separate triterpenes from caculis of Schisandra Chinensis (Turcz.) baill. A total of 89 mg corosolic acid with purities of 98.5% were obtained from 400 mg crude extract in one-step elution and less than 4 h, and the structure identification was performed by UV, IR, MS, 1H NMR and 13C NMR. The inhibition on liver fibrosis activities of the triterpenes against HSC-T6 in vitro were studied by cell culture methods. The results showed that the corosolic acid have better inhibitory effects on HSC-T6 cells with the IC50 value of 5~25 μg/mL and the study also indicated that corosolic acid might be a potential Chinese medical component to inhibit liver fibrosis.
authors.affiliation: College of Food Science Shenyang Agricultural University Dongling Road 120 Shenhe District, Shenyang China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Xianjun
authors.initials: X
authors.lastname: Meng
authors.forename: Lijie
authors.initials: L
authors.lastname: Zhu
authors.forename: Xinyao
authors.initials: X
authors.lastname: Jiao
authors.forename: Jiachen
authors.initials: J
authors.lastname: Zhang
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D017209:Apoptosis; D002460:Cell Line; D002478:Cells, Cultured; D002725:Chloroform; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D004867:Equipment Design; D005355:Fibrosis; D006801:Humans; D020128:Inhibitory Concentration 50; D008099:Liver / Q000473:pathology; D008103:Liver Cirrhosis / Q000188:drug therapy / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D000432:Methanol / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D032141:Schisandra / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013053:Spectrophotometry; D014315:Triterpenes / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D012997:Solvents; D014315:Triterpenes; D014867:Water; D002725:Chloroform; D020001:1-Butanol; C113861:corosolic acid; D000432:Methanol
keywords: Schisandra Chinensis (Turcz.) baill; liver fibrosis; triterpenes