title: Initiation and proliferation of carrot callus using a combination of antibiotics.
abstract: Calli were initiated from carrot (Daucus carota L. subsp. sativus Hoffm.) root expiants and grown on media supplemented with a combination of carbenicillin at 0.3 mg/ml and vancomycin at 0.1 mg/ml in the absence of plant hormones or hormone analogs. The growth rate was about half of that obtained with a combination of α-naphthaleneacetic acid and N(6)-benzyladenine at 1 mg/l each. Carbenicillin in the combination with vancomycin could be replaced by penicillin G; other penicillins tested in this combination, however, caused only limited growth. The calli produced can be grown on media supplemented with α-naphthaleneacetic acid and N(6)-benzyladenine, but not on media without the antibiotics and the plant growth substances. Calli obtained using the plant growth substances can also be subcultured on media supplemented with only the antibiotics.
authors.affiliation: Department of Biological Sciences, University of Wisconsin-Parkside, 53141-2000, Kenosha, WI, USA.
authors.forename: C C
authors.initials: CC
authors.lastname: Chang
authors.forename: D R
authors.initials: DR
authors.lastname: Schmidt