title: Unmodified gold nanoparticles as a simple colorimetric probe for ramoplanin detection.
abstract: In this paper unmodified gold nanoparticles (AuNPs) were used as a sensing element to detect ramoplamin. Detection relies on the fact that the dispersed AuNPs solution is red due to the intense surface plasmon absorption band at 530 nm whereas the AuNPs solution in the presence of ramoplanin is blue. Upon aggregation, there is a significant change in absorbance intensity at 620 nm. Based on the aggregation of AuNPs induced by ramoplanin, a simple colorimetric method was developed for determining the of ramoplanin concentration. Experimental conditions influencing the analytical performance such as particle size, amount of AuNPs, incubation time and pH were evaluated. Under the optimized experimental conditions, this method could detect ramoplanin in a linear range from 0.30 to 1.30 ppm with a detection limit of 0.01 ppm and exhibited good reproducibility, selectivity and recovery. Analysis time of this assay was only 2 min. To investigate its potential applicability, this assay was successfully applied for the determination of ramoplanin in urine samples without costly instruments.
authors.affiliation: Department of Chemistry, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathumthani 12110, Thailand. Electronic address: siriwan@mail.rmutt.ac.th.
authors.forename: Siriwan
authors.initials: S
authors.lastname: Teepoo
authors.forename: Phongnarin
authors.initials: P
authors.lastname: Chumsaeng
authors.forename: Khwankhao
authors.initials: K
authors.lastname: Palasak
authors.forename: Natvara
authors.initials: N
authors.lastname: Bousod
authors.forename: Naree
authors.initials: N
authors.lastname: Mhadbamrung
authors.forename: Phorntip
authors.initials: P
authors.lastname: Sae-lim
mesh_terms: D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000652:urine*; D003116:Color; D003124:Colorimetry / Q000191:economics / Q000379:methods*; D047630:Depsipeptides / Q000652:urine*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D015203:Reproducibility of Results; D020349:Surface Plasmon Resonance
chemical_list: D000900:Anti-Bacterial Agents; D047630:Depsipeptides; C041438:ramoplanin; D006046:Gold
keywords: Aggregation; Antibiotic drug; Gold nanoparticle; Ramoplanin