title: Regulation of galectin-3-induced apoptosis of Jurkat cells by both O-glycans and N-glycans on CD45.
abstract: Galectin-3 has been reported to induce apoptosis of Jurkat cells through binding receptors such as CD45. CD45RABC is heavily O-glycosylated and N-glycosylated, while CD45RO is only N-glycosylated. In this study, no apoptosis induced by galectin-3 was detected in CD45RO-transfected cells, whereas apoptosis of CD45RABC-transfected cells was observed, implying that O-glycans on CD45 might play roles in galectin-3-induced apoptosis. O-Glycosylation inhibition assay further suggests the role of O-glycans on CD45 in regulation of galectin-3-induced apoptosis. We also found that deglycosylation at N327 of CD45RO resulted in increased binding to galectin-3 without affecting apoptosis, while deglycosylation at N36 or N109 of CD45RO enhanced galectin-3-induced apoptosis. These data demonstrate that galectin-3-induced apoptosis of Jurkat cells is regulated by both O-glycans and N-glycans on CD45.
authors.affiliation: Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) and Comparative Medicine Center, Peking Union Medical College (PUMC), Key Laboratory of Human Disease Comparative Medicine, Beijing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xue
authors.forename: Xiqiang
authors.initials: X
authors.lastname: Gao
authors.forename: Chunyan
authors.initials: C
authors.lastname: Fu
authors.forename: Zhe
authors.initials: Z
authors.lastname: Cong
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Ting
authors.initials: T
authors.lastname: Chen
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wei
authors.forename: Chuan
authors.initials: C
authors.lastname: Qin
mesh_terms: D000595:Amino Acid Sequence; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D037502:Galectin 3 / Q000378:metabolism / Q000494:pharmacology*; D006031:Glycosylation / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D019169:Jurkat Cells; D017493:Leukocyte Common Antigens / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011134:Polysaccharides / Q000502:physiology*; D011485:Protein Binding / Q000187:drug effects / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D037502:Galectin 3; D011134:Polysaccharides; D017493:Leukocyte Common Antigens
keywords: Apoptosis; Binding; CD45; DMEM; Dulbecco’s modified Eagle’s medium; Galectin-3; MW; N-Glycans; O-Glycans; PBST; SDS–PAGE; molecular weight; phosphate buffered saline with Tween 20; sodium dodecyl sulfate–polyacrylamide gel electrophoresis