title: GPCR-mediated rapid, non-genomic actions of steroids: comparisons between DmDopEcR and GPER1 (GPR30).
abstract: Steroid hormones classically mediate their actions by binding to intracellular receptor proteins that migrate to the nucleus and act as transcription factors to change gene expression. However, evidence is now accumulating for rapid, non-genomic effects of steroids. There is considerable controversy over the mechanisms underlying such effects. In a number of cases evidence has been presented for the direct activation of G-protein coupled receptors (GPCRs) by steroids, either at the plasma membrane, or at intracellular locations. Here, we will focus on the non-genomic actions of ecdysteroids on a Drosophila GPCR, DopEcR (CG18314), which can be activated by both ecdysone and the catecholamine, dopamine. We will also point out parallels between this system and the activation of the vertebrate GPCR, GPER1 (GPR30), which is thought to be activated by 17β-estradiol. We propose that the cellular localization and signalling properties of both DopEcR and GPER1 may be cell specific and depend upon their interactions with both accessory molecules and signalling pathways.
authors.affiliation: The Inositide Laboratory, The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK. Electronic address: peter.evans@babraham.ac.uk.
authors.forename: Peter D
authors.initials: PD
authors.lastname: Evans
authors.affiliation: The Inositide Laboratory, The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK. Electronic address: asha.bayliss@babraham.ac.uk.
authors.forename: Asha
authors.initials: A
authors.lastname: Bayliss
authors.affiliation: The Inositide Laboratory, The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK. Electronic address: vincenzina.reale@babraham.ac.uk.
authors.forename: Vincenzina
authors.initials: V
authors.lastname: Reale
mesh_terms: D000818:Animals; D004298:Dopamine / Q000494:pharmacology*; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D026461:Ecdysteroids / Q000494:pharmacology*; D023281:Genomics; D006801:Humans; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D026461:Ecdysteroids; D043562:Receptors, G-Protein-Coupled; D011987:Receptors, Steroid; C034808:ecdysteroid receptor; D004298:Dopamine
keywords: Catecholamine; G-protein coupled receptor; Rapid, non-genomic actions; Steroid hormone