title: [Construction of aerobactin and sit operon mutant of avian pathogenic Escherichia coli E058 and evaluation of its pathogenicity].
abstract: OBJECTIVE
  To elucidate the pathogenesis role of the vir region of APEC 02 strain E058.
  
  
  METHODS
  The gene aerobactin/sitABC operon knockout mutants E058deltavir of APEC E058 strain was generated using Red recombination system. A series of pathogenicity tests including chick embryo inoculation, the competition experiment and the colonization and persistence in vivo were used to evaluate the pathogenicity of APEC E058deltavir and the wild-type strain E058.
  
  
  RESULTS
  E058deltavir was similar to its parental strain E058 in the growth curves, invasion assays of HD-11 cell and in vitro competition assay. In the colonization and persistence test, the recovery colonies of E058deltavir were significantly decreased in all of the organs tested (P < 0.001).
  
  
  CONCLUSIONS
  These results indicate that the virulence factors encoded by aerobactin/sit operon genes were important for the pathogenesis of APEC E058.
authors.affiliation: Key Laboratory of Avian Bioproducts Development, Ministry of Agriculture, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. lingjielu@126.com
authors.forename: Jielu
authors.initials: J
authors.lastname: Ling
authors.forename: Siyi
authors.initials: S
authors.lastname: Chen
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Gao
authors.forename: Huiqing
authors.initials: H
authors.lastname: Xu
authors.forename: Song
authors.initials: S
authors.lastname: Gao
authors.forename: Xiufan
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D002460:Cell Line; D002645:Chickens; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D006877:Hydroxamic Acids / Q000378:metabolism*; D009154:Mutation; D009876:Operon; D011201:Poultry Diseases / Q000382:microbiology*; D014774:Virulence
chemical_list: D029968:Escherichia coli Proteins; D006877:Hydroxamic Acids; C031819:aerobactin