title: Monothiol glutaredoxin-BolA interactions: redox control of Arabidopsis thaliana BolA2 and SufE1.
abstract: A functional relationship between monothiol glutaredoxins and BolAs has been unraveled by genomic analyses and in several high-throughput studies. Phylogenetic analyses coupled to transient expression of green fluorescent protein (GFP) fusions indicated that, in addition to the sulfurtransferase SufE1, which contains a C-terminal BolA domain, three BolA isoforms exist in Arabidopsis thaliana, BolA1 being plastidial, BolA2 nucleo-cytoplasmic, and BolA4 dual-targeted to mitochondria and plastids. Binary yeast two-hybrid experiments demonstrated that all BolAs and SufE1, via its BolA domain, can interact with all monothiol glutaredoxins. Most interactions between protein couples of the same subcellular compartment have been confirmed by bimolecular fluorescence complementation. In vitro experiments indicated that monothiol glutaredoxins could regulate the redox state of BolA2 and SufE1, both proteins possessing a single conserved reactive cysteine. Indeed, a glutathionylated form of SufE1 lost its capacity to activate the cysteine desulfurase, Nfs2, but it is reactivated by plastidial glutaredoxins. Besides, a monomeric glutathionylated form and a dimeric disulfide-bridged form of BolA2 can be preferentially reduced by the nucleo-cytoplasmic GrxS17. These results indicate that the glutaredoxin-BolA interaction occurs in several subcellular compartments and suggest that a redox regulation mechanism, disconnected from their capacity to form iron-sulfur cluster-bridged heterodimers, may be physiologically relevant for BolA2 and SufE1.
authors.affiliation: a Université de Lorraine, Interactions Arbres-Microorganismes, UMR1136, F-54500 Vandoeuvre-lès-Nancy, France.
authors.forename: Jérémy
authors.initials: J
authors.lastname: Couturier
authors.forename: Hui-Chen
authors.initials: HC
authors.lastname: Wu
authors.forename: Tiphaine
authors.initials: T
authors.lastname: Dhalleine
authors.forename: Henri
authors.initials: H
authors.lastname: Pégeot
authors.forename: Damien
authors.initials: D
authors.lastname: Sudre
authors.forename: José M
authors.initials: JM
authors.lastname: Gualberto
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Jacquot
authors.forename: Frédéric
authors.initials: F
authors.lastname: Gaymard
authors.forename: Florence
authors.initials: F
authors.lastname: Vignols
authors.forename: Nicolas
authors.initials: N
authors.lastname: Rouhier
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism*; D020134:Catalytic Domain; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D004789:Enzyme Activation; D054477:Glutaredoxins / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism; D010084:Oxidation-Reduction; D010788:Photosynthesis; D010802:Phylogeny; D011485:Protein Binding; D021381:Protein Transport; D013466:Sulfurtransferases / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C000589128:BolA protein, Arabidopsis; D004268:DNA-Binding Proteins; D054477:Glutaredoxins; D013466:Sulfurtransferases; C557797:sulfurtransferase 1, Arabidopsis
keywords: BolA; glutaredoxin; interaction; plants; redox control.