title: Purification of a novel peptide derived from a shellfish, Crassostrea gigas, and evaluation of its anticancer property.
abstract: A novel anticancer peptide was purified from Crassostrea gigas and investigated for its cytotoxic activity. To prepare the peptide, eight proteases were employed for enzymatic hydrolysis. Flavourzyme hydrolysate, which showed clearly superior cytotoxic activity on prostate cancer cells, was further purified using a membrane system and consecutive chromatographic methods. Finally, a novel anticancer peptide was obtained, and the sequence was partially purified as His-Phe-Asn-Ile-Gly-Asn-Arg-Cys-Leu-Cys at the N-terminus. The peptide purified from C. gigas effectively induced the death of prostate, breast, and lung cancer cells but not normal liver cells. This is the first report of an anticancer peptide derived from the enzymatic hydrolysates of C. gigas.
authors.affiliation: Department of Biotechnology, Konkuk University , Chungju 380-701, Republic of Korea.
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Cheong
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Kim
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Hwang
authors.forename: Yon-Suk
authors.initials: YS
authors.lastname: Kim
authors.forename: Jung-Suck
authors.initials: JS
authors.lastname: Lee
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Moon
authors.forename: Byong-Tae
authors.initials: BT
authors.lastname: Jeon
authors.forename: Pyo-Jam
authors.initials: PJ
authors.lastname: Park
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology; D045744:Cell Line, Tumor; D049896:Crassostrea / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D006868:Hydrolysis; D008175:Lung Neoplasms / Q000188:drug therapy / Q000473:pathology; D008297:Male; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000188:drug therapy
chemical_list: D000970:Antineoplastic Agents; D010455:Peptides