title: The effect of temperature on hairy root cultures of Catharanthus roseus: Growth, indole alkaloid accumulation and membrane lipid composition.
abstract: Cultivation of Catharanthus roseus hairy root cultures at different temperatures was found to have an effect on growth rate and indole alkaloid content as well as lipid composition. When lowering the temperature, the roots responded by increasing the degree of unsaturation of cellular lipids, which was mainly due to an increased proportion of linolenic acid in the main lipid classes. The modifications in lipid composition were obviously necessary for the roots to retain the proper cell membrane fluidity at each temperature. Despite of changes in membrane lipids, no effect on the distribution of indole alkaloids between the roots and the medium could be detected. Instead, the level of alkaloid accumulation showed a clear increase with lowering temperature.
authors.affiliation: Department of Chemical Engineering, Laboratory of Biochemistry and Microbiology, Helsinki University of Technology, Kemistintie 1, SF-02150, Espoo, Finland.
authors.forename: L
authors.initials: L
authors.lastname: Toivonen
authors.forename: S
authors.initials: S
authors.lastname: Laakso
authors.forename: H
authors.initials: H
authors.lastname: Rosenqvist