title: A ternary conjugation system for the construction of DNA libraries for Geobacillus kaustophilus HTA426.
abstract: This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.
authors.affiliation: Functional Genomics of Extremophiles, Faculty of Agriculture, Graduate School, Kyushu University.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Suzuki
authors.forename: Keisuke
authors.initials: K
authors.lastname: Wada
authors.forename: Megumi
authors.initials: M
authors.lastname: Furukawa
authors.forename: Katsumi
authors.initials: K
authors.lastname: Doi
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Ohshima
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D015723:Gene Library; D056506:Geobacillus / Q000235:genetics* / Q000378:metabolism; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D009964:Orotidine-5'-Phosphate Decarboxylase