title: In vivo application of poly-3-hydroxyoctanoate as peripheral nerve graft.
abstract: OBJECTIVE
  This study aims to investigate the degree of biocompatibility and neuroregeneration of a polymer tube, poly-3-hydroxyoctanoate (PHO) in nerve gap repair.
  
  
  METHODS
  Forty Wistar Albino male rats were randomized into two groups: autologous nerve gap repair group and PHO tube repair group. In each group, a 10-mm right sciatic nerve defect was created and reconstructed accordingly. Neuroregeneration was studied by sciatic function index (SFI), electromyography, and immunohistochemical studies on Days 7, 21, 45 and 60 of implantation. Biocompatibility was analyzed by the capsule formation around the conduit. Biodegradation was analyzed by the molecular weight loss in vivo.
  
  
  RESULTS
  Electrophysiological and histomorphometric assessments demonstrated neuroregeneration in both groups over time. In the experimental group, a straight alignment of the Schwann cells parallel to the axons was detected. However, autologous nerve graft seems to have a superior neuroregeneration compared to PHO grafts. Minor biodegradation was observed in PHO conduit at the end of 60 d.
  
  
  CONCLUSIONS
  Although neuroregeneration is detected in PHO grafts with minor degradation in 60 d, autologous nerve graft is found to be superior in axonal regeneration compared to PHO nerve tube grafts. PHO conduits were found to create minor inflammatory reaction in vivo, resulting in good soft tissue response.
authors.affiliation: Department of Neurosurgery, Faculty of Medicine, Muğla Sıtkı Koçman University, Muğla 48000, Turkey; Atatürk Research and Medical Center, Neurosurgery Clinic, Ministry of Health of the Republic of Turkey, Ankara 06110, Turkey; Department of Neurology, Faculty of Medicine, School of Medicine, Hacettepe University, Ankara 06100, Turkey; Department of Neurosurgery, Faculty of Medicine, School of Medicine, Hacettepe University, Ankara 06100, Turkey; Atatürk Research and Medical Center, Department of Pathology, Yıldırım Beyazıt University, Ankara 06110, Turkey; Department of Histology and Embryology, Faculty of Medicine, Muğla Sıtkı Koçman University, Muğla 48000, Turkey; Department of Chemistry, Bülent Ecevit University, Zonguldak 67100, Turkey.
authors.forename: D Burcu
authors.initials: DB
authors.lastname: Hazer
authors.forename: Ercan
authors.initials: E
authors.lastname: Bal
authors.forename: Gülay
authors.initials: G
authors.lastname: Nurlu
authors.forename: Kemal
authors.initials: K
authors.lastname: Benli
authors.forename: Serdar
authors.initials: S
authors.lastname: Balci
authors.forename: Feral
authors.initials: F
authors.lastname: Öztürk
authors.forename: Baki
authors.initials: B
authors.lastname: Hazer
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009416:Nerve Regeneration; D010525:Peripheral Nerves / Q000503:physiopathology / Q000637:transplantation*; D011091:Polyesters / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D011091:Polyesters; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C088151:poly(3-hydroxyoctanoic acid)