title: Disease-on-a-chip: mimicry of tumor growth in mammary ducts.
abstract: We present a disease-on-a-chip model in which cancer grows within phenotypically normal breast luminal epithelium on semicircular acrylic support mimicking portions of mammary ducts. The cells from tumor nodules developing within these hemichannels are morphologically distinct from their counterparts cultured on flat surfaces. Moreover, tumor nodules cocultured with the luminal epithelium in hemichannels display a different anticancer drug sensitivity compared to nodules cocultured with the luminal epithelium on a flat surface and to monocultures of tumor nodules. The mimicry of tumor development within the epithelial environment of mammary ducts provides a framework for the design and test of anticancer therapies.
authors.affiliation: Department of Basic Medical Sciences, Purdue University, 625 Harrison Street, Lynn Hall, West Lafayette, IN 47907-2026, USA. lelievre@purdue.edu jfleary@purdue.edu.
authors.forename: Pierre-Alexandre
authors.initials: PA
authors.lastname: Vidi
authors.forename: Teimour
authors.initials: T
authors.lastname: Maleki
authors.forename: Manuel
authors.initials: M
authors.lastname: Ochoa
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Sara M
authors.initials: SM
authors.lastname: Clark
authors.forename: James F
authors.initials: JF
authors.lastname: Leary
authors.forename: Sophie A
authors.initials: SA
authors.lastname: Lelièvre
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use; D001943:Breast Neoplasms / Q000188:drug therapy; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D004847:Epithelial Cells / Q000166:cytology; D005260:Female; D006801:Humans; D039503:Integrin alpha6 / Q000378:metabolism; D042361:Mammary Glands, Human / Q000166:cytology* / Q000378:metabolism; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D062826:Zonula Occludens-1 Protein / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D039503:Integrin alpha6; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein