title: Roles of Th17 cells in pulmonary granulomas induced by Schistosoma japonicum in C57BL/6 mice.
abstract: In schistosomiasis, limited information is available about the role of interleukin-17 (IL-17) in lung, despite the fact that this cytokine plays a crucial role during pro-inflammatory immune responses. In our study, we observed CD4(+)T cells changed after the infection. Furthermore, ELISA and FACS results revealed that Schistosomajaponicum infection could induce a large amount of IL-17 in mouse pulmonary lymphocytes. IL-17-producing cells, including Th17 cells, CD8(+)T (Tc) cells, γδT cells and natural killer T cells, was also associated with the development of lung inflammatory diseases. FACS results indicated that Th17 cell was the main source of IL-17 in the infected pulmonary lymphocytes after phorbol-12-myristate-13-acetate (PMA) and Ionomycin stimulation. Moreover, FACS results revealed that the percentage of Th17 cells continued to increase as over the course of S. japonicum infection. Additionally, cytokines co-expression results demonstrated that Th17 cells could express more IL-4 and IL-5 than IFN-γ. Reducing IL-17 activity by using anti-IL-17 ameliorated the damage and decreased infiltration of inflammatory cells in infected C57BL/6 mouse lungs. Collectively, these results suggest Th17 cells is the major IL-17-producing cells population and IL-17 contributes to pulmonary granulomatous inflammatory during the S. japonicum infection.
authors.affiliation: Department of Pathogenic Biology and Immunology, Guangzhou Medical University, 510182 Guangzhou, China. Electronic address: 2007141002@163.com.
authors.forename: Dianhui
authors.initials: D
authors.lastname: Chen
authors.forename: Hongyan
authors.initials: H
authors.lastname: Xie
authors.forename: Xueping
authors.initials: X
authors.lastname: Luo
authors.forename: Xiuxue
authors.initials: X
authors.lastname: Yu
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Fu
authors.forename: Haigang
authors.initials: H
authors.lastname: Gu
authors.forename: Changyou
authors.initials: C
authors.lastname: Wu
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Tang
authors.forename: Jun
authors.initials: J
authors.lastname: Huang
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D005260:Female; D015769:Granuloma, Respiratory Tract / Q000276:immunology*; D007249:Inflammation / Q000276:immunology; D007371:Interferon-gamma / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism*; D015847:Interleukin-4 / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D015759:Ionomycin / Q000378:metabolism; D008168:Lung / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D055611:Natural Killer T-Cells / Q000276:immunology; D010703:Phorbol Esters / Q000378:metabolism; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology* / Q000473:pathology; D058504:Th17 Cells / Q000276:immunology*
chemical_list: D020381:Interleukin-17; D015848:Interleukin-5; D010703:Phorbol Esters; D016692:Receptors, Antigen, T-Cell, gamma-delta; D015847:Interleukin-4; C119222:phorbol-12-myristate; D015759:Ionomycin; D007371:Interferon-gamma
keywords: Granulomas; Lung; Schistosoma japonicum; Th17