title: Proteomic analysis of Girdin-interacting proteins in migrating new neurons in the postnatal mouse brain.
abstract: Neural stem cells continuously generate new neurons in the ventricular-subventricular zone (V-SVZ) of the postnatal and adult mammalian brain. New neurons born in the rodent V-SVZ migrate toward the olfactory bulb (OB), where they differentiate into interneurons. To reveal novel intracellular molecular mechanisms that control postnatal neuronal migration, we performed a global proteomic search for proteins interacting with Girdin, an essential protein for postnatal neuronal migration. Using GST pull-down and LC-MS/MS shotgun analysis, we identified cytoskeletal proteins, cytoskeleton-binding proteins, and signal-transduction proteins as possible participants in neuronal migration. Our results suggest that Girdin and Girdin-interacting proteins control neuronal migration by regulating actin and/or microtubule dynamics.
authors.affiliation: Department of Developmental and Regenerative Biology, Nagoya City University, Graduate School of Medical Sciences, Aichi, Japan; Department of Anesthesiology and Medical Crisis Management, Nagoya City University, Graduate School of Medical Sciences, Aichi, Japan.
authors.forename: Haruko
authors.initials: H
authors.lastname: Ota
authors.forename: Takao
authors.initials: T
authors.lastname: Hikita
authors.forename: Tomoki
authors.initials: T
authors.lastname: Nishioka
authors.forename: Mami
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Jun
authors.initials: J
authors.lastname: Ito
authors.forename: Naoya
authors.initials: N
authors.lastname: Asai
authors.forename: Atsushi
authors.initials: A
authors.lastname: Enomoto
authors.forename: Masahide
authors.initials: M
authors.lastname: Takahashi
authors.forename: Kozo
authors.initials: K
authors.lastname: Kaibuchi
authors.forename: Kazuya
authors.initials: K
authors.lastname: Sobue
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Sawamoto
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000254:growth & development*; D002465:Cell Movement; D003598:Cytoskeletal Proteins / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008840:Microfilament Proteins / Q000378:metabolism*; D058953:Neural Stem Cells / Q000166:cytology / Q000502:physiology*; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D033921:Vesicular Transport Proteins / Q000378:metabolism*
chemical_list: D003598:Cytoskeletal Proteins; D008840:Microfilament Proteins; D033921:Vesicular Transport Proteins; C527550:Ccdc88a protein, mouse
keywords: Girdin; Liquid chromatography tandem mass spectrometry (LC–MS/MS); Neuronal migration; Postnatal neurogenesis; Ventricular–subventricular zone (V–SVZ)