title: Profiling of selected angiogenesis-related genes in proliferative eutopic endometrium of women with endometriosis.
abstract: OBJECTIVE
  To compare the expression level of the most relevant angiogenesis-related genes in the eutopic endometrium of women with and without endometriosis.
  
  
  METHODS
  32 regularly menstruating patients (18 with endometriosis and 14 controls) underwent surgery in the proliferative phase of the cycle. Eutopic endometrium was collected by the use of aspirating biopsy prior to laparoscopy. Only patients with advanced (stage III and IV) histopathologically confirmed ovarian endometriosis were studied. Real-time PCR gene arrays were applied to examine the expression of 84 human angiogenesis-connected genes. Western-blot and enzyme-linked immunosorbent assays (ELISA) were used to confirm the expression of selected proteins.
  
  
  RESULTS
  We found significantly higher levels of AKT1 (p=0.003), TYMP (p=0.02), JAG1 (p=0.007), LAMA5 (p=0.005) and TIMP-1 (p=0.03) in eutopic endometrium of patients with endometriosis as compared with controls. By the use of Western blot we found clearly positive expression of AKT1 whereas ELISA assays confirmed expression of AKT1, TYMP, JAG1, LAMA5 and TIMP1.
  
  
  CONCLUSIONS
  Changes in the expression of selected genes might lead to or be a consequence of an early defect in the physiological activity of proliferative endometrium ultimately resulting in its overgrowth outside the uterine cavity.
authors.affiliation: Department of Perinatology, Medical University of Bialystok, ul. Marii Sklodowskiej-Curie 24a, 15-276 Bialystok, Poland. Electronic address: plauda@umb.edu.pl.
authors.forename: P
authors.initials: P
authors.lastname: Laudanski
authors.affiliation: Department of Clinical Molecular Biology, Medical University of Bialystok, ul. Waszyngtona 13, 15-276 Bialystok, Poland.
authors.forename: R
authors.initials: R
authors.lastname: Charkiewicz
authors.affiliation: Department of Gynecology and Gynecological Oncology, Medical University of Bialystok, ul. Marii Sklodowskiej-Curie 24a, 15-276 Bialystok, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Kuzmicki
authors.affiliation: Department of Gynecology and Gynecological Oncology, Medical University of Bialystok, ul. Marii Sklodowskiej-Curie 24a, 15-276 Bialystok, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Szamatowicz
authors.affiliation: Department of Reproduction and Gynecological Endocrinology, Medical University of Bialystok, ul. Marii Sklodowskiej-Curie 24a, 15-276 Bialystok, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Świątecka
authors.affiliation: Department of Biochemical Diagnostics, Medical University of Bialystok, ul. Waszyngtona 15a, 15-276 Bialystok, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Mroczko
authors.affiliation: Department of Clinical Molecular Biology, Medical University of Bialystok, ul. Waszyngtona 13, 15-276 Bialystok, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Niklinski
mesh_terms: D000328:Adult; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D016022:Case-Control Studies; D004715:Endometriosis / Q000235:genetics* / Q000378:metabolism; D004717:Endometrium / Q000098:blood supply / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005498:Follicular Phase / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D000072100:Jagged-1 Protein; D007797:Laminin / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000235:genetics* / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000235:genetics*; D010049:Ovarian Diseases / Q000235:genetics* / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*; D060888:Real-Time Polymerase Chain Reaction; D000072099:Serrate-Jagged Proteins; D013939:Thymidine Phosphorylase / Q000235:genetics / Q000378:metabolism; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D055815:Young Adult
chemical_list: D002135:Calcium-Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; C000606273:JAG1 protein, human; D000072100:Jagged-1 Protein; D007797:Laminin; D008565:Membrane Proteins; D012333:RNA, Messenger; D000072099:Serrate-Jagged Proteins; C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; C097043:laminin alpha5; C543750:TYMP protein, human; D013939:Thymidine Phosphorylase; C494918:AKT1 protein, human; D051057:Proto-Oncogene Proteins c-akt
keywords: Angiogenesis; Endometriosis; Eutopic endometrium