title: MiR-26a enhances the radiosensitivity of glioblastoma multiforme cells through targeting of ataxia-telangiectasia mutated.
abstract: Glioblastoma multiforme (GBM) is notoriously resistant to radiation, and consequently, new radiosensitizers are urgently needed. MicroRNAs are a class of endogenous gene modulators with emerging roles in DNA repair. We found that overexpression of miR-26a can enhance radiosensitivity and reduce the DNA repair ability of U87 cells. However, knockdown miR-26a in U87 cells could act the converse manner. Mechanistically, this effect is mediated by direct targeting of miR-26a to the 3'UTR of ATM, which leads to reduced ATM levels and consequent inhibition of the homologous recombination repair pathway. These results suggest that miR-26a may act as a new radiosensitizer of GBM.
authors.affiliation: Department of Neurosurgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
authors.forename: Pin
authors.initials: P
authors.lastname: Guo
authors.affiliation: Department of Neurosurgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Lan
authors.affiliation: Department of Neurosurgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Ge
authors.affiliation: Department of Neurosurgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
authors.forename: Quanmin
authors.initials: Q
authors.lastname: Nie
authors.affiliation: Department of Neurosurgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
authors.forename: Liemei
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Neurosurgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China; Shanghai Institute of Head Trauma, Shanghai 200127, China. Electronic address: qiuzhoub@hotmail.com.
authors.forename: Yongming
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Department of Neurosurgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China; Shanghai Institute of Head Trauma, Shanghai 200127, China. Electronic address: maoq@netease.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Mao
mesh_terms: D000818:Animals; D064007:Ataxia Telangiectasia Mutated Proteins / Q000235:genetics*; D001932:Brain Neoplasms / Q000235:genetics* / Q000532:radiotherapy*; D004260:DNA Repair / Q000235:genetics / Q000528:radiation effects; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D018390:Gene Targeting; D005909:Glioblastoma / Q000235:genetics* / Q000532:radiotherapy*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000502:physiology*; D011836:Radiation Tolerance / Q000235:genetics*; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D064007:Ataxia Telangiectasia Mutated Proteins; D035683:MicroRNAs; C528163:MIRN26 microRNA, human; C576324:ATM protein, human
keywords: ATM; DNA repair; Glioblastoma multiforme; MiR-26a