title: Characterization and regeneration of salt- and water-stress mutants from protoplast culture of Nicotiana plumbaginifolia (Viviani).
abstract: Protoplast-derived colonies of haploid N. plumbaginifolia leaves were used to select for resistance to NaCl, KCl and polyethylene glycol 6000 (PEG). Salt-and PEG-tolerant cell lines were isolated on the basis of growth in a culture medium containing inhibitory concentrations of either NaCl or KCl (200 mM) or PEG (25%). The frequency of resistant lines ranged from 10(-5) to 10(-6). One resistant line from each treatment was regenerated into plants. All resistant lines produced 10-25 times more proline than the wild type when grown on a non-selective medium. Similar values were also observed in the leaves of resistant progeny plants. In each mutant line, salt or PEG resistance was transmitted as a single dominant nuclear gene as shown by segregation ratios in progenies of crosses between resistant and wild-type plants. The latter observation demonstrates clearly the existence of a genetic basis for increased salt tolerance.
authors.affiliation: Plantengenetica, Vrije Universiteit Brussel, Instituut voor Moleculaire Biologie, Paardenstraat 65, B-1640, St. Genesius Rode, Belgium.
authors.forename: S
authors.initials: S
authors.lastname: Sumaryati
authors.forename: I
authors.initials: I
authors.lastname: Negrutiu
authors.forename: M
authors.initials: M
authors.lastname: Jacobs