title: DNA delivery into Eucalyptus globulus zygotic embryos through biolistics: optimization of the biological and physical parameters of bombardment for two different particle guns.
abstract: DNA transfer into zygotic embryos of Eucalyptus globulus by microprojectile bombardment was studied with two devices: a gunpowder apparatus and a compressed-helium system. Using, as a test, the transient expression of a reporter gene, we optimized the physical and biological conditions of bombardment. Six-day-old cultured embryos were found to be the best target material, and osmotic treatment increased the expression rate. Conditions of bombardment (particle acceleration and quality of the particle: DNA mix) were studied. In optimal conditions, we were able to obtain up to 130 GUS expression events per embryo with a good distribution over the tissue.In our transient expression experiments, the gunpowder and helium devices exhibited similar efficiencies, reliabilities and reproducibilities.
authors.affiliation: Centre de Biologie et de Physiologie Végétale, URA CNRS 1457, Université Paul Sabatier, 118 Route de Narbonne, F-31062, Toulouse Cédex, France.
authors.forename: F
authors.initials: F
authors.lastname: Rochange
authors.forename: L
authors.initials: L
authors.lastname: Serrano
authors.forename: C
authors.initials: C
authors.lastname: Marque
authors.forename: C
authors.initials: C
authors.lastname: Teuliéres
authors.forename: A M
authors.initials: AM
authors.lastname: Boudet