title: Diversity of bacteriocinogenic lactic acid bacteria isolated from Mediterranean fish viscera.
abstract: Nine lactic acid bacteria strains showing bacteriocin-like activity were isolated from various fresh fish viscera. The following species were identified based on 16S rDNA sequences: Enterococcus durans (7 isolates), Lactococcus lactis (1) and Enterococcus faecium (1). These strains were active against Listeria innocua and other LAB. Random amplified polymorphic DNA analyses showed four major patterns for the E. durans species. PCR analyses revealed a nisin gene in the genome of the Lc. lactis strain. Genes coding enterocins A, B and P were found in the genome of the E. faecium isolate. Enterocins A and B genes were also present in the genome of E. durans GM19. Hence, this is the first report describing E. durans strains producing enterocins A and B. Electrospray ionization mass spectrometry revealed that the purified bacteriocin produced by the E. durans GMT18 strain had an exact molecular mass of 6,316.89 Da. This bacteriocin was designated as durancin GMT18. Edman sequencing failed to proceed; suggesting that durancin GTM18 may contain terminal lanthionine residues. Overall, the results obtained revealed the presence of a variety of enterococci in Mediterranean fish viscera, as evidenced by their genetic profiles and abilities to produce different bacteriocins. These strains could be useful for food biopreservation or as probiotics.
authors.affiliation: Département de Biochimie, Faculté de Médecine Ibn El Jazzar, Unité de Recherche U12-ES03, 4002, Sousse, Tunisia.
authors.forename: Sarra
authors.initials: S
authors.lastname: Migaw
authors.forename: Taoufik
authors.initials: T
authors.lastname: Ghrairi
authors.forename: Yanath
authors.initials: Y
authors.lastname: Belguesmia
authors.forename: Yvan
authors.initials: Y
authors.lastname: Choiset
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Berjeaud
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Chobert
authors.forename: Khaled
authors.initials: K
authors.lastname: Hani
authors.forename: Thomas
authors.initials: T
authors.lastname: Haertlé
mesh_terms: D000818:Animals; D001430:Bacteriocins / Q000378:metabolism*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005399:Fishes / Q000382:microbiology*; D019344:Lactic Acid / Q000378:metabolism; D056546:Lactobacillales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008522:Mediterranean Sea; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014781:Viscera / Q000382:microbiology
chemical_list: D001430:Bacteriocins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid