title: Dipeptidyl peptidase IV inhibitory peptides generated in Spanish dry-cured ham.
abstract: Dipeptidyl peptidase IV (DPP-IV) inhibitors are promising new therapies for type 2 diabetes. The aim of this study was to assay DPP-IV inhibitory peptides that can be present in a water soluble extract of Spanish dry-cured ham. Such an extract was fractionated by size-exclusion chromatography and the in vitro DPP-IV inhibitory activity determined in each collected fraction. Then, several peptides previously identified in dry-cured ham extracts or known to be products of DPP IV action were synthesised and assayed for DPP-IV inhibition. Peptides KA and AAATP showed the strongest DPP-IV inhibitory activity, with IC50 values of 6.27 mM and 6.47 mM, respectively. Dipeptides AA, GP, PL, and carnosine, as well as peptides AAAAG, ALGGA, and LVSGM were also DPP-IV inhibitors, although at a lower degree. These findings suggest the potential of Spanish dry-cured ham as a natural precursor of DPP-IV inhibitory peptides. These biopeptides could also be used as ingredients for functional foods or pharmaceutical products against type 2 diabetes.
authors.affiliation: Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Avenue Agustín Escardino 7, 46980 Paterna, Valencia, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Gallego
authors.forename: María-Concepción
authors.initials: MC
authors.lastname: Aristoy
authors.forename: Fidel
authors.initials: F
authors.lastname: Toldrá
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D003890:Desiccation; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism*; D054873:Dipeptidyl-Peptidase IV Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D005511:Food Handling / Q000379:methods*; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D008461:Meat Products / Q000032:analysis*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D013030:Spain; D013552:Swine
chemical_list: D054873:Dipeptidyl-Peptidase IV Inhibitors; D007004:Hypoglycemic Agents; D010455:Peptides; D018819:Dipeptidyl Peptidase 4
keywords: Bioactive peptides; Dipeptidyl peptidase IV inhibitor; Dry-cured ham; Type 2 diabetes