title: Calcium-dependent phospholipid-binding proteins in plants : Their characterisation and potential for regulating cell growth.
abstract: There is evidence that Ca(2+) can regulate vesicle-mediated secretion in plant cells, but the mechanism for this is not known. One possibility is that Ca(2+) -dependent phospholipid-binding proteins (annexins) couple the Ca(2+) stimulus to the exocytotic response. Using a protocol developed for the isolation of animal annexins we have identified proteins in maize (Zea mays L.) coleoptiles that have similar characteristics to annexins. The predominant polypeptide species run as a doublet of relative molecular mass (Mr) 33000-35000 on sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE); another less-abundant protein of Mr 23000 is also present. In the presence of Ca(2+) these proteins bind to liposomes composed of acidic phospholipids. Calcium-sensitivity of binding differs for each protein and is also influenced by the pH of the buffer used for the liposome-binding assay. Antiserum raised to the 33 to 35-kDa doublet purified on SDS-PAGE recognises the doublet in crude extracts from maize and proteins of similar Mr in Tradescantia virginiana and tobacco Nicotiana tabacum L. The antiserum also recognises p68 (Annexin VI) from chicken gizzard extracts, indicating homology between animal annexins and the maize proteins. For the maize proteins to be involved in the regulation of exocytosis, binding to phospholipids would be expected to occur at physiological levels of Ca(2+). The characteristics of the maize annexin-like proteins are described and attention drawn to the marked effect of pH in lowering the requirement for Ca(2+) for phospholipid binding.
authors.affiliation: Department of Horticulture, School of Plant Sciences, University of Reading, Whiteknights, RG6 2AS, Reading.
authors.forename: H D
authors.initials: HD
authors.lastname: Blackbourn
authors.forename: J H
authors.initials: JH
authors.lastname: Walker
authors.forename: N H
authors.initials: NH
authors.lastname: Battey