title: Molecular phylogeny analysis and species identification of Dendrobium (Orchidaceae) in China.
abstract: Dendrobium plants are important commercial herbs in China, widely used in traditional medicine and ornamental horticulture. In this study, sequence-related amplified polymorphism (SRAP) markers were applied to molecular phylogeny analysis and species identification of 31 Chinese Dendrobium species. Fourteen SRAP primer pairs produced 727 loci, 97% of which (706) showed polymorphism. Average polymorphism information content of the SRAP pairs was 0.987 (0.982-0.991), showing that plenty of genetic diversity exists at the interspecies level of Chinese Dendrobium. The molecular phylogeny analysis (UPGMA) grouped the 31 Dendrobium species into six clusters. We obtained 18 species-specific markers, which can be used to identify 10 of the 31 species. Our results indicate the SRAP marker system is informative and would facilitate further application in germplasm appraisal, evolution, and genetic diversity studies in the genus Dendrobium.
authors.affiliation: Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou, 310018, China, Shangguo007@126.com.
authors.forename: Shang-Guo
authors.initials: SG
authors.lastname: Feng
authors.forename: Jiang-Jie
authors.initials: JJ
authors.lastname: Lu
authors.forename: Ling
authors.initials: L
authors.lastname: Gao
authors.forename: Jun-Jun
authors.initials: JJ
authors.lastname: Liu
authors.forename: Hui-Zhong
authors.initials: HZ
authors.lastname: Wang
mesh_terms: D002681:China; D018744:DNA, Plant / Q000235:genetics*; D031667:Dendrobium / Q000145:classification* / Q000235:genetics*; D005819:Genetic Markers; D014644:Genetic Variation; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics; D011110:Polymorphism, Genetic; D013045:Species Specificity
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers