title: Phosphorylation of Thr(1495) of nestin in a mouse model of cerebral ischemia and reperfusion damage.
abstract: Nestin, a class VI intermediate filament protein, is expressed by neuronal progenitor cells in the subventricular zone (SVZ). In the present study, we analyzed the nestin expression and phosphorylation levels in nerve cells in a mouse model of cerebral ischemia and reperfusion. C57BL/6 mice were subjected to three-vessel occlusion for 14 min, and were killed either 1 or 4 days after the procedure. The percentages of cells in the SVZ that were positive for nestin, Thr(1495)-phosphorylated nestin or Ki67 did not significantly differ between the ischemic reperfusion and sham groups. Conversely, in the striatum and cornu ammonis 2 (CA2) regions, the mice at 4 days after ischemic reperfusion showed significantly higher numbers and percentages of nerve cells that were positive for nestin, Thr(1495)-phosphorylated nestin and Ki67 compared to results from the other groups. To our knowledge, this is the first description of phosphorylated nestin expression in neural progenitor cells in the SVZ of adult mice. In this cerebral ischemia and reperfusion mouse model, cells positive for Thr(1495)-phosphorylated nestin were increased in the striatum and CA2 field of the hippocampus; suggesting that nestin phosphorylation may play an important role in mitotically active neuronal progenitor cells.
authors.affiliation: Department of Pathology and Integrative Oncological Pathology, Nippon Medical School, Tokyo, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Matsuda
authors.forename: Go
authors.initials: G
authors.lastname: Suzuki
authors.forename: Teruo
authors.initials: T
authors.lastname: Kusano
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kawamoto
authors.forename: Hisashi
authors.initials: H
authors.lastname: Yoshimura
authors.forename: Akira
authors.initials: A
authors.lastname: Fuse
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yokota
authors.forename: Zenya
authors.initials: Z
authors.lastname: Naito
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Ishiwata
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D064231:Nestin / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation / Q000502:physiology; D010770:Phosphotransferases / Q000378:metabolism*; D015427:Reperfusion Injury / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism*; D013912:Threonine / Q000378:metabolism
chemical_list: D064231:Nestin; D013912:Threonine; D010770:Phosphotransferases
keywords: ischemia and reperfusion damage; nestin; neurogenesis; phosphorylation