title: Synthesis and characterization of poly[1-(N,N-bis-carboxymethyl)amino-3-allylglycerol-co-dimethylacrylamide] grafted to magnetic nano-particles for extraction and determination of letrozole in biological and pharmaceutical samples.
abstract: In this paper, a new method is reported for the surface grafting of poly[1-(N,N-bis-carboxymethyl)amino-3-allylglycerol-co-dimethylacrylamide] onto magnetic nano-particles modified by 3-mercaptopropyltrimethoxysilane. The grafted nano-sorbent was characterized by Fourier transform infrared spectroscopy, elemental analysis, thermogravimetric analysis, and scanning electron microscopy. Agglomerated nano-particles with multi-pores were used for extraction and determination of trace letrozole in human biological fluids and pharmaceutical samples. The profile of the letrozole uptake by the magnetic nano-sorbent reflected good accessibility of the active sites in the grafted polymer. Scatchard analysis revealed that the sorption capacity of the functionalized nano-sorbent was 6.27 µmol g(-1) at an optimum pH of 4. The equilibrium adsorption data of letrozole by grafted magnetic nano-sorbent were analyzed by Langmuir, Freundlich, Temkin and Redlich-Peterson models. Conformation of the experimental data in the Langmuir isotherm model indicated the homogeneous binding site of functional polymer-grafted magnetic nano-sorbent surface. Nearly 89% of letrozole was released in simulated gastric fluid, pH 1.2, in 2h and 79% in simulated intestinal fluid, pH 7.4, in 30 h. These results show the utility of the letrozole loaded- polymer grafted magnetite nano-particles for enteric drug delivery.
authors.affiliation: Department of Chemistry, Central Tehran Branch, Islamic Azad University, Tehran, Iran. Electronic address: h.ahmadpanahi@iauctb.ac.ir.
authors.forename: Homayon
authors.initials: H
authors.lastname: Ahmad Panahi
authors.forename: Elham Reza
authors.initials: ER
authors.lastname: Soltani
authors.forename: Elham
authors.initials: E
authors.lastname: Moniri
authors.forename: Atefeh
authors.initials: A
authors.lastname: Tamadon
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D000970:Antineoplastic Agents / Q000097:blood* / Q000737:chemistry / Q000652:urine; D001665:Binding Sites; D004337:Drug Carriers / Q000032:analysis* / Q000138:chemical synthesis; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D000077289:Letrozole; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D009570:Nitriles / Q000097:blood* / Q000737:chemistry / Q000652:urine; D017646:Organosilicon Compounds; D016062:Porosity; D012821:Silanes / Q000737:chemistry*; D052616:Solid Phase Extraction; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D014230:Triazoles / Q000097:blood* / Q000737:chemistry / Q000652:urine
chemical_list: D000180:Acrylic Resins; D000970:Antineoplastic Agents; D004337:Drug Carriers; D058185:Magnetite Nanoparticles; D009570:Nitriles; D017646:Organosilicon Compounds; D012821:Silanes; D014230:Triazoles; C552859:poly(1-(N,N-bis-carboxymethyl)amino-3-allylglycerol-co-dimethylacrylamide)copolymer; D000077289:Letrozole; C102833:(3-mercaptopropyl)trimethoxysilane
keywords: Breast cancer; Enteric drug delivery; Human biological fluids; Letrozole; Pharmaceutical samples; Solid phase extraction