title: Effect of virus infection on symplastic transport of fluorescent tracers in Nicotiana clevelandii leaf epidermis.
abstract: The molecular weight exclusion limit of plasmodesmata in subveinal epidermal cells of Nicotiana clevelandii (Gray) leaves was estimated by microinjection and fluorescence microscopy using fluorescein isothiocyanate-peptide conjugates, carboxyfluorescein and Lucifer Yellow CH. The largest fluorochrome which moved symplastically between cells had a molecular weight of 749, although movement did not appear to depend purely on molecular weight parameters. Systemic infection of plants by tobacco rattle tobravirus, tomato black ring nepovirus or potato Y potyvirus did not alter the limits of plasmodesmatal conductance of the fluorochromes. However, carrot mottle umbravirus and groundnut rosette umbravirus diminished the symplastic mobility of some fluorescent tracers. These results imply that intercellular movement of these viruses does not involve a long-lasting increase in the plasmodesmatal molecular size exclusion limit.
authors.affiliation: Scottish Crop Research Institute, Invergowrie, DD2 5DA, Dundee, UK.
authors.forename: P M
authors.initials: PM
authors.lastname: Derrick
authors.forename: H
authors.initials: H
authors.lastname: Barker
authors.forename: K J
authors.initials: KJ
authors.lastname: Oparka