title: PTK2 and PTPN11 expression in myelodysplastic syndromes.
abstract: OBJECTIVE
  The aim of this study was to evaluate the expression of protein tyrosine kinase 2 and protein tyrosine phosphatase non-receptor type 11, which respectively encode focal adhesion kinase protein and src homology 2 domain-containing protein-tyrosine phosphatase 2, in hematopoietic cells from patients with myelodysplastic syndromes.
  
  
  METHODS
  Protein tyrosine kinase 2 and tyrosine phosphatase non-receptor type 11 expressions were analyzed by quantitative polymerase chain reaction in bone marrow cells from patients with myelodysplastic syndromes and healthy donors.
  
  
  RESULTS
  Protein tyrosine kinase 2 and tyrosine phosphatase non-receptor type 11 expressions did not significantly differ between normal cells and myelodysplastic cells.
  
  
  CONCLUSIONS
  Our data suggest that despite the relevance of focal adhesion kinase and src homology 2 domain-containing protein-tyrosine phosphatase 2 in hematopoietic disorders, their mRNA expression do not significantly differ between total bone marrow cells from patients with myelodysplastic syndromes and healthy donors.
authors.affiliation: Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, CampinasSP, Brazil.
authors.forename: Mariana
authors.initials: M
authors.lastname: Lazarini
authors.forename: João Agostinho
authors.initials: JA
authors.lastname: Machado-Neto
authors.forename: Leticia Fröhlich
authors.initials: LF
authors.lastname: Archangelo
authors.forename: Bruna Fernandes
authors.initials: BF
authors.lastname: Mendes-Silva
authors.forename: Carolina Louzão
authors.initials: CL
authors.lastname: Bigarella
authors.forename: Fabiola
authors.initials: F
authors.lastname: Traina
authors.forename: Sara Teresinha Olalla
authors.initials: ST
authors.lastname: Saad
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001854:Bone Marrow Cells / Q000378:metabolism*; D005260:Female; D051418:Focal Adhesion Kinase 2 / Q000032:analysis / Q000378:metabolism*; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000032:analysis / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011379:Prognosis; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11 / Q000032:analysis* / Q000378:metabolism; D012307:Risk Factors; D018709:Statistics, Nonparametric; D055815:Young Adult; D018909:src Homology Domains / Q000502:physiology
chemical_list: D051418:Focal Adhesion Kinase 2; D051416:Focal Adhesion Protein-Tyrosine Kinases; C103547:PTK2B protein, human; C516724:PTPN11 protein, human; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11