title: INS-1 cells inhibit the production of extracellular matrix from pancreatic stellate cells.
abstract: In type 2 diabetes mellitus, pancreatic stellate cells (PSCs) are present within and surrounding pancreatic islets and may cause progressive fibrosis and deterioration of pancreatic beta cell function. However, it is unknown whether pancreatic beta cells influence the biological behavior of PSCs. In the present study, we examined the impact of pancreatic beta cells on the proliferation, migration and extracellular matrix (ECM) production of PSCs. PSCs were treated with conditioned media from INS-1 cells (supernatant, SN). Although the proliferation of PSCs incubated with INS-1-SN was increased compared to control, INS-1-SN treatment induced matrix metalloproteinase-2 activity and reduced the production of ECM and TGF-β1. In addition, PSCs treated with INS-1-SN reduced the secretion of cytokines that are known to mediate pancreatic beta cell death, such as FADD, Fas, IFN-γ, IL-1, TNF-α, and TRAIL. Our findings suggest that pancreatic beta cells may ameliorate islet fibrosis and the progression of islet dysfunction.
authors.affiliation: Department of Endocrinology, Institute of Diabetes, Zhongda Hospital, Medical School, Southeast University, No. 87 Dingjiaqiao, Nanjing, 210009, China.
authors.forename: Fengfei
authors.initials: F
authors.lastname: Li
authors.forename: Bijun
authors.initials: B
authors.lastname: Chen
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Zha
authors.forename: S
authors.initials: S
authors.lastname: Zhou
authors.forename: Tongzhi
authors.initials: T
authors.lastname: Wu
authors.forename: M G
authors.initials: MG
authors.lastname: Bachem
authors.forename: Zilin
authors.initials: Z
authors.lastname: Sun
mesh_terms: D000818:Animals; D002450:Cell Communication; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D016207:Cytokines / Q000096:biosynthesis; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D058954:Pancreatic Stellate Cells / Q000378:metabolism*; D051381:Rats
chemical_list: D016207:Cytokines