title: Nuclear genes affecting percentage of green plants in barley (Hordeum vulgare L.) anther culture.
abstract: The genetics behind response in barley anther culture was studied with 22 reciprocal and one single: cross between three varieties with high and four varieties with low capacity for green plant formation. Effects of genotypes dominated embryo formation and percentages of green plants, accounting for 62 and 76% of total variation, respectively, with almost no genetic effect on the ability to regenerate plants from pollen embryos. Nuclear genes could explain all genotype effects in this plant material, since no reciprocal effects were indicated. The three parents with high and the four parents with low capacity for green plant formation formed two phenotypically homogeneous groups, producing 27-52% and 0-7% green plants, respectively. Genetic variation within hybrids for both embryo and green plant formation could be explained completely by general combining ability (GCA). The results are discussed with respect to a previous similar study in hexaploid wheat and the reported existence of DNA deletions in the plastid genomes in albino plants from anther culture of wheat and barley.
authors.affiliation: Department of Agricultural Science, Section of Plant Breeding and Biotechnology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871, Frederiksberg C, Denmark.
authors.forename: E T
authors.initials: ET
authors.lastname: Larsen
authors.forename: I K
authors.initials: IK
authors.lastname: Tuvesson
authors.forename: S B
authors.initials: SB
authors.lastname: Andersen