title: Cytological and molecular characterization of a chromosome interchange and addition lines in Cadet involving chromosome 5B of wheat and 6Ag of Lophopyrum ponticum.
abstract: Efforts to transfer wheat curl mite (Eriophyes tulipae Keifer) resistance from Lophopyrum ponticum 10X (Podb.) Love to bread wheat (Triticum aestivum L.) have resulted in the production of a number of cytogenetic stocks, including an addition line of 6Ag, a "ditelo" addition line, and a wheat-Lophopyrum translocation line. Characterization of these lines with C-banding, in situ hybridization with a Lophopyrum species-specific repetitive DNA probe (pLeUCD2), and Southern blotting with pLeUCD2 and a 5S ribosomal DNA probe (pScT7) confirmed that the distal portion of the short arm of 6Ag was translocated onto the distal portion of 5BS (5BL. 5BS-6AgS). It was also determined that the "ditelo" addition was an acrocentric chromosome of 6AgS.
authors.affiliation: Plant Research Centre, Research Branch, Agriculture Canada, K1A OC6, Ottawa, Canada.
authors.forename: N S
authors.initials: NS
authors.lastname: Kim
authors.forename: K C
authors.initials: KC
authors.lastname: Armstrong
authors.forename: G
authors.initials: G
authors.lastname: Fedak
authors.forename: A
authors.initials: A
authors.lastname: Fominaya
authors.forename: E W
authors.initials: EW
authors.lastname: Whelan