title: Induction of immune response and prevention of alveolar bone loss with recombinant Porphyromonas gingivalis peptidylarginine deiminase.
abstract: OBJECTIVE
  Treatments for periodontitis are not absolutely perfect, and a vaccine against Porphyromonas gingivalis (P. gingivalis) could become a valuable adjunct therapy for periodontitis.
  
  
  METHODS
  In this study, a vaccine of peptidylarginine deiminase (PAD) from P. gingivalis was evaluated in P. gingivalis-induced murine lesion and periodontitis models. The prevention of alveolar bone loss analysis determined by micro-computed X-ray tomography (micro-CT), and histological assays. Furthermore, the induction of immune response of mouse anti-PAD done with ELISA and Western Blot analysis.
  
  
  RESULTS
  Compared with animal immunization with incomplete Freund's adjuvant (IFA) alone, PAD group significantly inhibited (P<0.05) bone resorption. ELISA and Western Blot showed that PAD induced response involving immunoglobulin G1 (Ig G1) predominantly.
  
  
  CONCLUSIONS
  These results suggest that PAD could be a candidate antigen for a vaccine against P. gingivalis infection.
authors.affiliation: Department of Periodontology, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Shaanxi 710004, China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Zhu
authors.forename: Jin
authors.initials: J
authors.lastname: Yang
authors.forename: Junyi
authors.initials: J
authors.lastname: Sun
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Shi
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Gou
authors.forename: Ang
authors.initials: A
authors.lastname: Li
mesh_terms: D016301:Alveolar Bone Loss / Q000000981:diagnostic imaging / Q000276:immunology* / Q000517:prevention & control; D000704:Analysis of Variance; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology* / Q000302:isolation & purification; D001428:Bacterial Vaccines; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology; D006867:Hydrolases / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010518:Periodontitis / Q000276:immunology* / Q000517:prevention & control; D016966:Porphyromonas gingivalis / Q000276:immunology*; D000076342:Protein-Arginine Deiminases; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D019444:Vaccines, DNA / Q000096:biosynthesis / Q000276:immunology*; D055114:X-Ray Microtomography
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA; D006867:Hydrolases; D000076342:Protein-Arginine Deiminases
keywords: Effective protection; Peptidylarginine deiminase; Periodontitis; Porphyromonas gingivalis; Vaccine