title: Identification of a 32-kDa anther marker protein for androgenic response in maize, Zea mays L.
abstract: Variations in the whole anther protein pattern have been investigated in a highly androgenic maize hybrid during the inductive pretreatment for androgenesis. It was found that a 32-kDa protein (MAR32) is induced and accumulates in the anthers during cold pretreatment of the tassel. A positive correlation between the rate of embryo formation via anther culture and the level of this protein after 7 days of cold treatment was observed. In addition, the in vivo synthesis of this protein by cold-pretreated anthers was demonstrated. Different responsive and non-responsive genotypes were also evaluated, and the accumulation of MAR32-like protein was only observed in certain responsive genotypes. The results suggest that the protein MAR32 is a marker for a form of androgenic responsiveness in maize.
authors.affiliation: Reconnaissance Cellulaire et Amélioration des Plantes, INRA 23879, EP 20 CNRS, Université Lyon 1, 69622, Villeurbanne Cedex, France.
authors.forename: P
authors.initials: P
authors.lastname: Vergne
authors.forename: F
authors.initials: F
authors.lastname: Riccardi
authors.forename: M
authors.initials: M
authors.lastname: Beckert
authors.forename: C
authors.initials: C
authors.lastname: Dumas