title: Lin28 enhances tissue repair by reprogramming cellular metabolism.
abstract: Regeneration capacity declines with age, but why juvenile organisms show enhanced tissue repair remains unexplained. Lin28a, a highly conserved RNA-binding protein expressed during embryogenesis, plays roles in development, pluripotency, and metabolism. To determine whether Lin28a might influence tissue repair in adults, we engineered the reactivation of Lin28a expression in several models of tissue injury. Lin28a reactivation improved hair regrowth by promoting anagen in hair follicles and accelerated regrowth of cartilage, bone, and mesenchyme after ear and digit injuries. Lin28a inhibits let-7 microRNA biogenesis; however, let-7 repression was necessary but insufficient to enhance repair. Lin28a bound to and enhanced the translation of mRNAs for several metabolic enzymes, thereby increasing glycolysis and oxidative phosphorylation (OxPhos). Lin28a-mediated enhancement of tissue repair was negated by OxPhos inhibition, whereas a pharmacologically induced increase in OxPhos enhanced repair. Thus, Lin28a enhances tissue repair in some adult tissues by reprogramming cellular bioenergetics. PAPERCLIP:
authors.affiliation: Stem Cell Transplantation Program, Division of Pediatric Hematology/Oncology, Boston Children's Hospital and Dana-Farber Cancer Institute, Boston, MA 02115, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA; Harvard Stem Cell Institute, Boston, MA 02115, USA; Manton Center for Orphan Disease Research, Boston, MA 02115, USA; Howard Hughes Medical Institute, Boston, MA 02115, USA; Department of Medicine, Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA.
authors.forename: Ng
authors.initials: N
authors.lastname: Shyh-Chang
authors.forename: Hao
authors.initials: H
authors.lastname: Zhu
authors.forename: T
authors.initials: T
authors.lastname: Yvanka de Soysa
authors.forename: Gen
authors.initials: G
authors.lastname: Shinoda
authors.forename: Marc T
authors.initials: MT
authors.lastname: Seligson
authors.forename: Kaloyan M
authors.initials: KM
authors.lastname: Tsanov
authors.forename: Liem
authors.initials: L
authors.lastname: Nguyen
authors.forename: John M
authors.initials: JM
authors.lastname: Asara
authors.forename: Lewis C
authors.initials: LC
authors.lastname: Cantley
authors.forename: George Q
authors.initials: GQ
authors.lastname: Daley
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000378:metabolism; D004734:Energy Metabolism; D005121:Extremities / Q000502:physiology; D018859:Hair Follicle / Q000502:physiology; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012038:Regeneration; D014945:Wound Healing
chemical_list: C488352:Lin-28 protein, mouse; D035683:MicroRNAs; D016601:RNA-Binding Proteins; C542687:mirnlet7 microRNA, mouse