title: High frequency plant regeneration from anther-derived cell suspension cultures via somatic embryogenesis in Catharanthus roseus.
abstract: A system for high frequency plant regeneration from cell suspension cultures in Catharanthus roseus is described. Calli were obtained from anthers cultured on Murashige and Skoog's medium supplemented with 1 mgl(-1) α-naphthaleneacetic acid and 0.1 mgl(-1) kinetin. After the second subculture on solid medium, embryogenic callus was identified and transferred to liquid medium to initiate suspension cultures. Cells dispersed finely in the medium were subcultured at 14-day intervals. Upon plating onto the basal medium, yellowish compact colonies proliferated from the cells and more than 80% of them gave rise to somatic embryos. Subsequently, plantlets developed from the embryos. Both the plantlets and the source plants showed the normal somatic chromosome number of 2n=2x=16.
authors.affiliation: Plant Cell Biology Lab., Genetic Engineering Research Institute, KIST, P.O. Box 17, 305-606, Taedok Science Town, Taejon, Korea.
authors.forename: S W
authors.initials: SW
authors.lastname: Kim
authors.forename: N H
authors.initials: NH
authors.lastname: Song
authors.forename: K H
authors.initials: KH
authors.lastname: Jung
authors.forename: S S
authors.initials: SS
authors.lastname: Kwak
authors.forename: J R
authors.initials: JR
authors.lastname: Liu