title: Effects of the kar gene on cytoplasmic mixing and mitochondrial genome suppressiveness, and consequences for cytoduction of petite DNA in Saccharomyces cerevisiae.
abstract: During a series of cytoduction experiments to transfer Saccharomyces cerevisiae mitochondrial genomes from one nuclear background to another, using the karl-1 nuclear fusion mutation, one of the five petite genomes used proved difficult to transfer. This genome, ϱ(-) F13, was highly suppressive (90%) in its original nuclear background. Molecular and genetic studies on the putative karl-1 ϱ(-)F13 cytoductant were done to discover the nature of this difficulty. They showed that while the ϱ(-)F13 was maintained in a karl-l background, zygotes from a mating with a ϱ(0) strain showed poor cytoplasmic mixing and therefore inefficient ϱ(-)F 13 DNA transfer into first zygotic buds. This also caused a reduction of ϱ(-)F13 suppressiveness to 20-30% in crosses with different ϱ(+) strains. The effect was genome specific since another highly suppressive petite in the karl-l background did not show suppressiveness reduction when crossed to ϱ(+). The nature of suppressiveness modulation is discussed. Since the ϱ(-)F13 genome was eventually transferred using a modification of the original scheme, the problems were not caused by the inability of the acceptor nuclear background to maintain the ϱ(-)F13 genome.
authors.affiliation: Department of Biology, Case Western Reserve University, 44106, Cleveland, Ohio, USA.
authors.forename: E P
authors.initials: EP
authors.lastname: Sena