title: Characterization of a soluble B7-H3 (sB7-H3) spliced from the intron and analysis of sB7-H3 in the sera of patients with hepatocellular carcinoma.
abstract: B7-H3 is a recently discovered member of the B7 superfamily molecules and has been found to play a negative role in T cell responses. In this study, we identified a new B7-H3 isoform that is produced by alternative splicing from the forth intron of B7-H3 and encodes the sB7-H3 protein. Protein sequence analysis showed that sB7-H3 contains an additional four amino acids, encoded by the intron sequence, at the C-terminus compared to the ectodomain of 2Ig-B7-H3. We further found that this spliced sB7-H3 plays a negative regulatory role in T cell responses and serum sB7-H3 is higher in patients with hepatocellular carcinoma (HCC) than in healthy donors. Furthermore, we found that the expression of the spliced sb7-h3 gene is higher in carcinoma and peritumor tissues than in PBMCs of both healthy controls and patients, indicating that the high level of serum sB7-H3 in patients with HCC is caused by the increased expression of this newly discovered spliced sB7-H3 isoform in carcinoma and peritumor tissues.
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China ; Treatment and Research Center for Infectious Diseases, 302 Hospital of P.L.A., Beijing, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Chen
authors.forename: Peixin
authors.initials: P
authors.lastname: Liu
authors.forename: Yedong
authors.initials: Y
authors.lastname: Wang
authors.forename: Weimin
authors.initials: W
authors.lastname: Nie
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Li
authors.forename: Wen
authors.initials: W
authors.lastname: Xu
authors.forename: Fengyi
authors.initials: F
authors.lastname: Li
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Zhou
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
authors.forename: Henggui
authors.initials: H
authors.lastname: Liu
mesh_terms: D000328:Adult; D017398:Alternative Splicing / Q000235:genetics; D000595:Amino Acid Sequence; D060887:B7 Antigens / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000097:blood*; D002681:China; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D047668:Inteins / Q000235:genetics; D008113:Liver Neoplasms / Q000097:blood*; D008297:Male; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000097:blood / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D060887:B7 Antigens; C423237:CD276 protein, human; D017931:DNA Primers; D020033:Protein Isoforms