title: Biological insights into BRAFV600mutations in melanoma patient: Not mere therapeutic targets.
abstract: Some experimental evidence indicates that uncommonBRAFmutations consisting in the substitution of 2 adjacent nucleotides within codon 600 are in acisconfiguration and associate withBRAFgene amplification. These findings suggest thatBRAFV600mutations are unlikely to occur as homozygous alterations in clinical melanoma samples, with gene amplification perhaps contributing to mask the heterozygous state.
authors.affiliation: Laboratory of Clinical Research and Molecular Diagnostics; IRCCS - CROB Centro di Riferimento Oncologico della Basilicata; Rionero in Vulture (Pz), Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Improta
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Pelosi
authors.forename: Elena
authors.initials: E
authors.lastname: Tamborini
authors.forename: Marco
authors.initials: M
authors.lastname: Donia
authors.forename: Mario
authors.initials: M
authors.lastname: Santinami
authors.forename: Filippo
authors.initials: F
authors.lastname: de Braud
authors.forename: Filippo
authors.initials: F
authors.lastname: Fraggetta
keywords: BRAF; MHC; homozygosis; immunotherapy; melanoma; vemurafenib