title: Neuroprotective effect of Panax notoginseng plysaccharides against focal cerebral ischemia reperfusion injury in rats.
abstract: Our present study was conducted to investigate whether Panax notoginseng plysaccharides (PNPS) exerted a neuroprotective effect against focal cerebral ischemia/reperfusion (I/R) injury in rats. Before mice were subjected to middle cerebral artery occlusion (MCAO) for 2 h and reperfusion for 22 h, PNPS at the doses of 50, 100, and 200 mg/kg was administered once a day intragastrically for continuous 7 days. Oral administration of PNPS could significantly reduce the severity of neurological deficits, infarct volumes, cerebral edema, and neuronal death caused by MCAO in rats. Moreover, in the presence of PNPS, the Bcl-2/Bax ratio increased, but the level of cleaved caspase-3 reduced. Thus, these finding suggested that suppressing apoptosis through increasing Bcl-2/Bax ratio and evoking caspase-3 cascade should be potential mechanism by which PNPS exerts its neuroprotective function against focal cerebral I/R injury.
authors.affiliation: Department of Neurosurgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Jia
authors.affiliation: Pediatric Intensive Care Unit, Xi'an Children's Hospital, Xi'an 710003, China.
authors.forename: Yangbin
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Neurology, the Ninth Hospital of Xi'an, Xi'an 710054, China.
authors.forename: Junxian
authors.initials: J
authors.lastname: Gao
authors.affiliation: Pediatric Intensive Care Unit, Xi'an Children's Hospital, Xi'an 710003, China.
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Pediatric Intensive Care Unit, Xi'an Children's Hospital, Xi'an 710003, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Chu
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Xi'an Medical University, Xi'an 710077, China. Electronic address: yashu_xamu@outlook.com.
authors.forename: Ya
authors.initials: Y
authors.lastname: Shu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002545:Brain Ischemia / Q000188:drug therapy; D006801:Humans; D051379:Mice; D018696:Neuroprotective Agents / Q000008:administration & dosage* / Q000737:chemistry; D053723:Panax notoginseng / Q000737:chemistry; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D051381:Rats; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology
chemical_list: D018696:Neuroprotective Agents; D011134:Polysaccharides
keywords: Apoptosis; Cerebral ischemia reperfusion; Cerebroprotective effect; Panax notoginseng; Polysaccharide