title: Restriction fragment length polymorphism analysis of loci associated with disease resistance genes and developmental traits in Pisum sativum L.
abstract: An F2 population of pea (Pisum sativum L.) consisting of 174 plants was analysed by restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) techniques. Ascochyta pisi race C resistance, plant height, flowering earliness and number of nodes were measured in order to map the genes responsible for their variation. We have constructed a partial linkage map including 3 morphological character genes, 4 disease resistance genes, 56 RFLP loci, 4 microsatellite loci and 2 RAPD loci. Molecular markers linked to each resistance gene were found: Fusarium wilt (6 cM from Fw), powdery mildew (11 cM from er) and pea common Mosaic virus (15 cM from mo). QTLs (quantitative traits loci) for Ascochyta pisi race C resistance were mapped, with most of the variation explained by only three chromosomal regions. The QTL with the largest effect, on chromosome 4, was also mapped using a qualitative, Mendelian approach. Another QTL displayed a transgressive segregation, i.e. the parental line that was susceptible to Ascochyta blight had a resistance allele at this QTL. Analysis of correlations between developmental traits in terms of QTL effects and positions suggested a common genetic control of the number of nodes and earliness, and a loose relationship between these traits and height.
authors.affiliation: Station de Génétique Végétale, Université Paris Sud/INRA/CNRS URA 1492, La Ferme du Moulon, F-91190, Gif-sur-Yvette, France.
authors.forename: E
authors.initials: E
authors.lastname: Dirlewanger
authors.forename: P G
authors.initials: PG
authors.lastname: Isaac
authors.forename: S
authors.initials: S
authors.lastname: Ranade
authors.forename: M
authors.initials: M
authors.lastname: Belajouza
authors.forename: R
authors.initials: R
authors.lastname: Cousin
authors.forename: D
authors.initials: D
authors.lastname: de Vienne