title: Quantitation of sweet steviol glycosides by means of a HILIC-MS/MS-SIDA approach.
abstract: Meeting the rising consumer demand for natural food ingredients, steviol glycosides, the sweet principle of Stevia rebaudiana Bertoni (Bertoni), have recently been approved as food additives in the European Union. As regulatory constraints require sensitive methods to analyze the sweet-tasting steviol glycosides in foods and beverages, a HILIC-MS/MS method was developed enabling the accurate and reliable quantitation of the major steviol glycosides stevioside, rebaudiosides A-F, steviolbioside, rubusoside, and dulcoside A by using the corresponding deuterated 16,17-dihydrosteviol glycosides as suitable internal standards. This quantitation not only enables the analysis of the individual steviol glycosides in foods and beverages but also can support the optimization of breeding and postharvest downstream processing of Stevia plants to produce preferentially sweet and least bitter tasting Stevia extracts.
authors.affiliation: Chair of Food Chemistry and Molecular Sensory Science, Technische Universität München , Lise-Meitner-Straße 34, D-85354 Freising-Weihenstephan, Germany.
authors.forename: Caroline
authors.initials: C
authors.lastname: Well
authors.forename: Oliver
authors.initials: O
authors.lastname: Frank
authors.forename: Thomas
authors.initials: T
authors.lastname: Hofmann
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D045786:Diterpenes, Kaurane / Q000032:analysis* / Q000737:chemistry; D005504:Food Analysis / Q000379:methods; D005960:Glucosides / Q000032:analysis; D006027:Glycosides / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D012015:Reference Standards; D012680:Sensitivity and Specificity; D036625:Stevia / Q000737:chemistry; D013549:Sweetening Agents / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards
chemical_list: D045786:Diterpenes, Kaurane; D005960:Glucosides; D006027:Glycosides; D013549:Sweetening Agents; C074471:steviolbioside; C012043:stevioside; C012042:steviol; C454559:rebaudioside F; C056177:rubusoside