title: Comparison of open and retroperitonoscopic donor nephrectomy in terms of lipid and protein peroxidation responses.
abstract: BACKGROUND
  This study was designed to compare donors who underwent open (ODN) versus retroperitonoscopic nephrectomy (RDN) in terms of intra-operative oxidative stress and recipients graft function in the early postoperative period.
  
  
  METHODS
  Among 40 patients who underwent donor nephrectomy, 23 were operated via an open method and 17 via retroperitonoscopic method. To analyze oxidative stress, we measured plasma levels of malondialdehyde (MDA), protein carbonyl, and protein sulfhydryl moieties in donor venous blood before induction of anesthesia and postoperatively at 0, 6, and 24 hours. The influence of oxidative stress on graft function was evaluated by means of the postoperative 5th day recipient creatinine and estimated glomerular filtration rate (eGFR) Modification of Diet in Renal Disease Formula (MDRD) to evaluate delayed graft function (DGF) status.
  
  
  RESULTS
  ODN patients showed significantly higher 24-hour mean levels of MDA, (6,139 ± 1,854 vs 4,813 ± 1,771 nmol/L; P = .01), protein carbonyl (366 ± 64 vs 311 ± 62 μmol/L; P = .01) and protein sulfhydryl (468 ± 110 vs 386 ± 75 μmol/L; P = .01) moieties compared with those RDN patients. However, ODN and RDN recipients were similar in terms of 5th day mean creatinine and eGFR (1.1 ± 0.3 vs 1.4 ± 0.8 mg/dL and 69.15 ± 12.24 vs 56.31 ± 25.2, respectively) and DGF status (4.4% [1/23] vs 5.9% [1/17], respectively).
  
  
  CONCLUSIONS
  Although ODN donors were more prone to intra-operative oxidative stress than RDN donors, based on significantly higher levels of oxidative stress markers, this difference seems to not significantly influence recipients early graft function.
authors.affiliation: Organ Transplantation Institute, Akdeniz University Faculty of Medicine, Antalya, Turkey. Electronic address: adinckan@akdeniz.edu.tr.
authors.forename: A
authors.initials: A
authors.lastname: Dinckan
authors.forename: B
authors.initials: B
authors.lastname: Dinc
authors.forename: S
authors.initials: S
authors.lastname: Turkyilmaz
authors.forename: A
authors.initials: A
authors.lastname: Tekin
authors.forename: H
authors.initials: H
authors.lastname: Kocak
authors.forename: H
authors.initials: H
authors.lastname: Akbas
authors.forename: A
authors.initials: A
authors.lastname: Mesci
authors.forename: M
authors.initials: M
authors.lastname: Saracoğlu
authors.forename: C
authors.initials: C
authors.lastname: Polat
authors.forename: A
authors.initials: A
authors.lastname: Kahraman
authors.forename: N
authors.initials: N
authors.lastname: Hadimioglu
authors.forename: A
authors.initials: A
authors.lastname: Gurkan
mesh_terms: D000328:Adult; D003404:Creatinine / Q000097:blood; D005260:Female; D005919:Glomerular Filtration Rate; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D009392:Nephrectomy / Q000379:methods*; D018384:Oxidative Stress; D010537:Peritoneum / Q000601:surgery*; D011506:Proteins / Q000378:metabolism*; D014019:Tissue Donors
chemical_list: D011506:Proteins; D008315:Malondialdehyde; D003404:Creatinine