title: Znf45l affects primitive hematopoiesis by regulating transforming growth factor-β signaling.
abstract: Znf45l, containing classical C2H2 domains, is a novel member of Zinc finger proteins in zebrafish. In vertebrates, TGF-β signaling plays a critical role in hematopoiesis. Here, we showed that Znf45l is expressed both maternally and zygotically throughout early development. Znf45l-depleted Zebrafish embryos display shorter tails and necrosis with reduced expression of hematopoietic maker genes. Furthermore, we revealed that znf45l locates downstream of TGF-β ligands and maintains normal level of TGF-β receptor type II phosphorylation. In brief, our results indicate that znf45l affects initial hematopoietic development through regulation of TGF-β signaling.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Chen
authors.forename: Huaqin
authors.initials: H
authors.lastname: Sun
authors.forename: Dachang
authors.initials: D
authors.lastname: Tao
authors.forename: Ping
authors.initials: P
authors.lastname: Yang
authors.forename: Shasha
authors.initials: S
authors.lastname: Bian
authors.forename: Yunqiang
authors.initials: Y
authors.lastname: Liu
authors.forename: Sizhong
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China mayongxin@gmail.com.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000378:metabolism; D047108:Embryonic Development; D009025:Morpholines / Q000378:metabolism; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D015027:Zebrafish / Q000254:growth & development; D029961:Zebrafish Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D009025:Morpholines; D012333:RNA, Messenger; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D029961:Zebrafish Proteins; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II