title: Nanoslit membrane-integrated fluidic chip for protein detection based on size-dependent particle trapping.
abstract: This paper describes the fabrication of a nanoslit membrane-integrated fluidic chip (Nanoslit-Chip) used for trapping and concentrating micro-/nano-particles of desired size and its application in detecting biological molecules based on target-induced particle aggregation. To trap particles of a specific size, a large scale uniform sized nanoslit fluid channel array is fabricated on a silicon dioxide membrane. A small number of fluorescence labeled particles in a large volume of solution are concentrated into a monolayer of particles in a small nanoslit membrane, which enables us to effectively quantify them via fluorescence intensity. In addition, the particles of desired size (1.8 μm) are readily separated from the mixture of particles with a different size (450 nm) in Nanoslit-Chip size, and then quantified via fluorescence measurements. Finally, the Nanoslit-Chip is successfully applied to the sensitive detection of proteins by target-induced particle aggregation, trapping, and quantification. This shows its potential as a biological and clinical device for quantitative and sensitive detection of biological molecules.
authors.affiliation: School of Electrical Engineering and Computer Science, Seoul National University, Seoul 151-742, Republic of Korea. yongkkim@snu.ac.kr.
authors.forename: Yul
authors.initials: Y
authors.lastname: Koh
authors.forename: Homan
authors.initials: H
authors.lastname: Kang
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Lee
authors.forename: Jin-Kyoung
authors.initials: JK
authors.lastname: Yang
authors.forename: Jong-Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Yoon-Sik
authors.initials: YS
authors.lastname: Lee
authors.forename: Yong-Kweon
authors.initials: YK
authors.lastname: Kim
mesh_terms: D000818:Animals; D019921:Biotinylation; D002417:Cattle; D005456:Fluorescent Dyes / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D011485:Protein Binding; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000378:metabolism; D019809:Streptavidin / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins; D012710:Serum Albumin, Bovine; D019809:Streptavidin