title: Compositional equivalence of DAS-444Ø6-6 (AAD-12 + 2mEPSPS + PAT) herbicide-tolerant soybean and nontransgenic soybean.
abstract: Soybeans from transgenic event DAS-444Ø6-6 are the first to express three proteins that provide tolerance to broad-spectrum herbicides. DAS-444Ø6-6 soybean expresses the aryloxyalkanoate dioxygenase-12 (AAD-12) enzyme from the soil bacterium Delftia acidovorans , which provides tolerance to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D); the double-mutant 5-enolpyruvylshikimate-3-phosphate synthase (2mEPSPS) enzyme encoded by a modified version of the epsps gene from maize ( Zea mays ), which provides tolerance to the herbicide glyphosate; and the phosphinothricin acetyltransferase (PAT) enzyme from Streptomyces viridochromogenes , which provides tolerance to the herbicide glufosinate. The purpose of this study was to determine if the nutrient and antinutrient composition of forage and grain from DAS-444Ø6-6 soybean are similar to those of nontransgenic soybean. Forage was analyzed for proximates, fiber, and minerals; grain analyses further included vitamins, amino acid and fatty acid profiles, and antinutrients and bioactive components (lectin, phytic acid, raffinose, stachyose, trypsin inhibitor, and isoflavones). Results indicate that DAS-444Ø6-6 soybean is compositionally equivalent to nontransgenic soybean. Findings are consistent with similar studies for other input traits, as endogenous plant metabolic pathways that influence composition are expected to be less affected by transgenesis compared with traditional plant-breeding methods.
authors.affiliation: Dow AgroSciences LLC , 9330 Zionsville Road, Indianapolis, Indiana 46268, United States.
authors.forename: Miles D
authors.initials: MD
authors.lastname: Lepping
authors.forename: Rod A
authors.initials: RA
authors.lastname: Herman
authors.forename: Brian L
authors.initials: BL
authors.lastname: Potts
mesh_terms: D000596:Amino Acids / Q000032:analysis / Q000378:metabolism; D004043:Dietary Fiber / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D030841:Food, Genetically Modified; D053209:Herbicide Resistance; D006540:Herbicides / Q000494:pharmacology; D008903:Minerals / Q000032:analysis / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
chemical_list: D000596:Amino Acids; D004043:Dietary Fiber; D005227:Fatty Acids; D006540:Herbicides; D008903:Minerals