title: Insulin and IGF-I receptors and tyrosine kinase activity in carp ovaries: changes with reproductive cycle.
abstract: Insulin and insulin-like growth factor I (IGF-I) receptors from carp ovaries were semipurified with wheat germ agglutinin at different moments of the reproductive cycle and their binding characteristics and tyrosine kinase activity were studied. Specific receptors for insulin and IGF-I were found. IGF-I receptors presented higher binding (23.8 ± 1.5%), number of receptors (965 ± 20fm/mg) and affinity (KD 0.24 ± 0.03nM) than those shown for insulin receptors (4.1 ± 1%, 530 ± 85fm/mg and 0.85 ± 0.1nM, respectively). Insulin and IGF-I receptors have a tyrosine kinase activity which is not different from that found in muscle of the same species. Seasonal changes were found in binding, with maximum values for insulin and IGF-I reached at the end of pre-spawning period (June). However, while IGF-I binding was observed in all stages, insulin binding decreased in autumn and disappeared in winter, which suggests a different role for the two peptides in ovarian physiology.
authors.affiliation: Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Gutiérrez
authors.forename: M
authors.initials: M
authors.lastname: Párrizas
authors.forename: N
authors.initials: N
authors.lastname: Carneiro
authors.forename: J L
authors.initials: JL
authors.lastname: Maestro
authors.forename: M A
authors.initials: MA
authors.lastname: Maestro
authors.forename: J
authors.initials: J
authors.lastname: Planas