title: Dectin-1 Stimulation Selectively Reinforces LPS-driven IgG1 Production by Mouse B Cells.
abstract: Dectin-1, which specifically recognizes β-glucan of fungal cell walls, is a non-Toll-like receptor (TLR) pattern recognition receptor and a representative of C-type lectin receptors (CLRs). The importance of Dectin-1 in innate immune cells, such as dendritic cells and macrophages, has previously been well studied. However, the function of Dectin-1 in B cells is very poorly understood. To determine the role of Dectin-1 in B cell activation, we first investigated whether mouse B cells express Dectin-1 and then assessed the effect of Dectin-1 stimulation on B cell proliferation and antibody production. Mouse B cells express mRNAs encoding CLRs, including Dectin-1, and surface Dectin-1 was expressed in B cells of C57BL/6 rather than BALB/c strain. Dectin-1 agonists, heat-killed Candida albicans (HKCA) and heat-killed Saccharomyces cerevisiae (HKSC), alone induced B cell proliferation but not antibody production. Interestingly, HKSC, HKCA, and depleted zymosan (a selective Dectin-1 agonist) selectively enhanced LPS-driven IgG1 production. Taken together, these results suggest that, during fungal infection, β-glucan-stimulated Dectin-1 may cooperate with TLR4 to specifically enhance IgG1 production by mouse B cells.
authors.affiliation: Department of Microbiology, Myunggok Medical Research Institute, College of Medicine, Konyang University, Daejeon 302-718, Korea.
authors.forename: Beom-Seok
authors.initials: BS
authors.lastname: Seo
authors.forename: Sang-Hoon
authors.initials: SH
authors.lastname: Lee
authors.forename: Ju-Eon
authors.initials: JE
authors.lastname: Lee
authors.forename: Yung-Choon
authors.initials: YC
authors.lastname: Yoo
authors.forename: Junglim
authors.initials: J
authors.lastname: Lee
authors.forename: Seok-Rae
authors.initials: SR
authors.lastname: Park
keywords: B cell; C-type lectin receptor; Dectin-1; Fungal immunity; IgG1