title: Angiotensin-converting enzyme gene insertion/deletion polymorphism studies in Asian Indian pregnant women biochemically identifies gestational diabetes mellitus.
abstract: BACKGROUND
  Gestational diabetes mellitus (GDM) is defined as glucose intolerance first recognized during pregnancy. Insertion/deletion (I/D) polymorphism of a 287 bp Alu repetitive sequence in intron 16 of the angiotensin-converting enzyme (ACE) gene has been widely investigated in Asian Indian populations with different ethnic origins. The present study examined possible association between I/D polymorphism of the ACE gene and GDM in Asian Indian pregnant women.
  
  
  METHODS
  A total of 200 pregnant women (100 GDM and 100 non-GDM) were recruited in this study and I/D polymorphism of a 287 bp Alu1 element inside intron 16 of the ACE gene was examined by polymerase chain reaction (PCR)-based gel electrophoresis.
  
  
  RESULTS
  The distribution of the variants like II, ID, and DD genotypes of ACE gene showed differences between normal GDM versus non-GDM subjects, and the frequency of the ID+ DD Vs II genotype was significant (p=0.0002) in the GDM group.
  
  
  CONCLUSIONS
  ACE gene polymorphism was associated with GDM in Asian Indian pregnant women.
authors.affiliation: Department of Genetics and Molecular Medicine, Kamineni Hospitals, India Department of Genetics, Vasavi Medical and Research Centre, India Department of Genetics and Biotechnology, Osmania University, India.
authors.forename: Imran A
authors.initials: IA
authors.lastname: Khan
authors.affiliation: Department of Genetics and Biotechnology, Osmania University, India.
authors.forename: Parveen
authors.initials: P
authors.lastname: Jahan
authors.affiliation: Department of Genetics and Molecular Medicine, Kamineni Hospitals, India Department of Genetics, Vasavi Medical and Research Centre, India.
authors.forename: Qurratulain
authors.initials: Q
authors.lastname: Hasan
authors.affiliation: Department of Genetics and Molecular Medicine, Kamineni Hospitals, India Department of Biochemistry, Kamineni Hospitals, India Department of Biochemistry, Kasturba Medical College, Manipal University, India drpragnarao@gmail.com.
authors.forename: Pragna
authors.initials: P
authors.lastname: Rao
mesh_terms: D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D016640:Diabetes, Gestational / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005951:Glucose Tolerance Test; D006801:Humans; D054643:INDEL Mutation / Q000235:genetics*; D007194:India; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D011110:Polymorphism, Genetic; D011247:Pregnancy; D012307:Risk Factors; D055815:Young Adult
chemical_list: D007703:Peptidyl-Dipeptidase A
keywords: Gestational diabetes mellitus; angiotensin-converting enzyme; insertion/deletion polymorphism; polymerase chain reaction; type 2 diabetes mellitus