title: Polyvinylamine-G-galactose is a route to bioactivated silica surfaces.
abstract: Polyvinylamine (PVAm) was derivatized with lactobionic acid to give PVAm-GAL with pendant galactose groups along the PVAm chain. The galactose substituents were shown to undergo two types of specific interactions: (1) condensation with phenylboronic acid moieties on polymers and on surfaces; and, (2) the specific binding of RCA120, a galactose-specific lectin. Surface binding and assembly was monitored with Quartz Crystal Microbalance (QCM-D) measurements. Multilayer assemblies based on boronic ester formation were destroyed by lowering the pH, or by introducing sorbitol. We propose that the physical adsorption of PVAm-GAL onto silica or other negatively charged support surfaces is a simple route to galactose-rich interfaces, possibly useful for affinity separations, cell targeting and cell culturing.
authors.affiliation: Department of Chemical Engineering, McMaster University, Hamilton, Ontario L8S 4L7, Canada.
authors.forename: Hajir
authors.initials: H
authors.lastname: Mokhtari
authors.forename: Robert
authors.initials: R
authors.lastname: Pelton
authors.forename: Liqiang
authors.initials: L
authors.lastname: Jin
mesh_terms: D005690:Galactose / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011145:Polyvinyls / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D011145:Polyvinyls; C534904:polyvinylamine; D012822:Silicon Dioxide; D005690:Galactose
keywords: Boronate complexes; Galactose; Layer-by-layer; Lectins; Polyvinylamine; QCM-D