title: Topical application of a Chinese medicine, Qingpeng ointment, ameliorates 2, 4-dinitrofluorobenzene-induced allergic contact dermatitis in BALB/c mice.
abstract: BACKGROUND
  Qingpeng ointment (QP) is a traditional Chinese medicine which has been used for treatment of eczema in China. However, the mechanisms of its anti-inflammatory activities are not known.
  
  
  OBJECTIVE
  To assess the anti-inflammatory effects of QP using a mouse model of allergic contact dermatitis, compare the effects with mometasone furoate cream (MF), and to relate the effects to modulation of inflammatory cytokines.
  
  
  METHODS
  Persistent dermatitis was induced in BALB/c mice using dinitrofluorobenzene. Topical treatment, including the vehicle of QP, 50%, 75%, 100% QP in vehicle, and MF was applied for 14 days. Dermatitis was evaluated macroscopically and microscopically at day 8 and day 15 after treatment. The levels of IL1B, IL2, TNFA, IFNG in both sera and skin tissue were detected with Enzyme-Linked Immunosorbent Assay (ELISA).
  
  
  RESULTS
  Significant reductions of skin inflammation in mice skin were observed after treatment with QP and MF, but not with the vehicle of QP. Similar to MF, QP also suppressed the expression of IL1B, IL2, TNFA and IFNG.
  
  
  CONCLUSIONS
  This study demonstrates that QP inhibits allergic contact dermatitis in mice, similar to MF. QP suppresses the expression of Th1 cytokines in both sera and skin tissue, by which it may exert its anti-inflammatory effects.
authors.affiliation: Department of Dermatology, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing, China, 100191.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Dermatology, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing, China, 100191.
authors.forename: Lin-Feng
authors.initials: LF
authors.lastname: Li
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D017449:Dermatitis, Allergic Contact / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D004139:Dinitrofluorobenzene; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D007371:Interferon-gamma / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D000068656:Mometasone Furoate; D009824:Ointments; D011244:Pregnadienediols / Q000627:therapeutic use; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D053583:Interleukin-1beta; D007376:Interleukin-2; D009824:Ointments; D011244:Pregnadienediols; C540321:Qingpeng plaster; D014409:Tumor Necrosis Factor-alpha; D000068656:Mometasone Furoate; D007371:Interferon-gamma; D004139:Dinitrofluorobenzene
keywords: Allergic contact dermatitis; Qingpeng ointment; dinitrofluorobenzene; mouse; traditional Chinese medicine