title: Influence of medium and cold pretreatment on androgenetic response in Lolium perenne L.
abstract: Genotypes of Lolium perenne L. with different androgenetic responses were used to test effects of induction medium composition. The media tested were potato II (pII), 190-2, and modified Linsmaier and Skoog media, LS-1, LS-2, and LS-3. The effect of different gelling agents, activated charcoal in a 'double layer' design, and casein hydrolysate were also studied. From 36,696 anthers, 25,906 embryo-like structures, 1,959 albino and 173 green plants were generated. Significant differences were found between media, genotypes and medium-genotype interactions studied. All three media commonly used, pII, 190-2, and LS-3, were equivalent in production of green plants. Cold pretreatment of the anthers (4°C) significantly increased the number of embryo-like structures, the number and proportion of albino plants produced, but not the production of green plants.
authors.affiliation: Department of Biotechnological Sciences, Agricultural University of Norway, POB 5040, N-1432, Ås, Norway.
authors.forename: H G
authors.initials: HG
authors.lastname: Opsahl-Ferstad
authors.forename: A
authors.initials: A
authors.lastname: Bjørnstad
authors.forename: O A
authors.initials: OA
authors.lastname: Rognli