title: Protection of a new heptapeptide from Carapax trionycis against carbon tetrachloride-induced acute liver injury in mice.
abstract: A new heptapeptide GAGPHGG (OC1) was isolated from Carapax trionycis which was a traditional Chinese medicine (TCM) used for treatment of hepatic diseases. The structure of OC1 was characterized by MS, NMR techniques, together with amino acid sequence analysis. The hepatoprotective activity of OC1 was evaluated in vivo using the CCl4-induced acute liver injury model. Combining the pathological examination and the biochemical assays, OC1 (0.34 mg/kg, hypodermic injection) displayed better hepatoprotective effect than bifendate (100 mg/kg, intragastric administration) against the acute liver injury induced by carbon tetrachloride (CCl4) in mice. Compared with the model group, OC1 could signiﬁcantly suppress the increase of serum level of aminotransferase (alanine transaminase (ALT) and aspartate aminotransferase (AST)), decrease the formation of malondialdehyde (MDA) and elevate the activity of glutathione peroxidase (GSH-Px) in liver (p<0.01). And the acute toxic test showed that median lethal dose (LD50) of OC1 exceeded 6.8 mg/kg, via hypodermic injection in mice.
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine.
authors.forename: Penglong
authors.initials: P
authors.lastname: Wang
authors.forename: Yuzhong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yawen
authors.initials: Y
authors.lastname: An
authors.forename: Kuo
authors.initials: K
authors.lastname: Xu
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.forename: Chao
authors.initials: C
authors.lastname: Fu
authors.forename: Jinxuan
authors.initials: J
authors.lastname: Lin
authors.forename: Shixun
authors.initials: S
authors.lastname: Xu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Haimin
authors.initials: H
authors.lastname: Lei
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D060105:Animal Shells / Q000378:metabolism*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001835:Body Weight; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D009842:Oligopeptides / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use; D020011:Protective Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use; D014426:Turtles
chemical_list: D004365:Drugs, Chinese Herbal; D009842:Oligopeptides; D020011:Protective Agents; C000588775:glycyl-alanyl-glycyl-phenylalanyl-histidyl-glycyl-glycine; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D005979:Glutathione Peroxidase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase