title: Establishment of dsDNA/GNs/chit/GCE biosensor and electrochemical study on interaction between 6-mercaptopurine and DNA.
abstract: In this paper, the anti-cancer drug 6-mercaptopurine (6-MP) was taken as the detection object. The biosensor of dsDNA/GNs/chit/GCE was established using the grapheme (GNs) and chitosan (chit) as the compound modified material. The electrochemical behavior of 6-MP on the sensor was discussed, and the damage and its mechanism of 6-MP on DNA were studied. The experimental result showed that, after the modification of GNs-chit, the electrode activation area of GNs/chit/GCE increased remarkably, which was improved from 1.76cm2 to 8.64 cm2, and the responsive oxidation peak current of GNs/chit/GCE to K3[Fe(CN)6] also increased remarkably. At the meantime, it was demonstrated that DNA was effectively fixed on the GNs/chit/GCE electrode;6-MP caused obvious damage to dsDNA, and the damage degree on the adenine was bigger than that on the guanine; the interaction between 6-MP and dsDNA was preliminarily deduced as the intercalation, and its electrochemical oxidation process was an irreversible process controlled by the adsorption.
authors.affiliation: Department of Chemistry, Tongji University, Shanghai, 200092, China.
authors.forename: Wenwei
authors.initials: W
authors.lastname: Tang
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Xinping
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000225:Adenine / Q000737:chemistry; D000327:Adsorption; D015374:Biosensing Techniques; D048271:Chitosan / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D016903:Drug Monitoring; D017097:Electric Impedance; D004563:Electrochemistry; D004566:Electrodes; D006108:Graphite / Q000737:chemistry; D006147:Guanine / Q000737:chemistry; D006801:Humans; D015122:Mercaptopurine / Q000737:chemistry*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry
chemical_list: D006147:Guanine; D006108:Graphite; D004247:DNA; D048271:Chitosan; D015122:Mercaptopurine; D000225:Adenine; D010100:Oxygen
keywords: 6-MP; Biosensor; DNA damage; grapheme; intercalation