title: Plant regeneration from callus cultures of Valeriana wallichii DC.
abstract: Petiole expiants of Valeriana wallichii. DC., a threatened medicinal plant, were used for inducing callus. Optimum callus formation was observed on Murashige and Skoogs' (1962) medium supplemented with 3.0 mg/l NAA and 0.25 mg/l Kn. Shoot regeneration was achieved upon transferring the callus to medium containing 1.0 mg/l Kn and 0.25 mg/l NAA. Complete plantlets were obtained on the same medium or upon transfer of the regenerated shoot buds to medium containing 5.0 mg/l Kn and 1.0 mg/l IAA. Nearly a thousand callus regenerated plants were successfully transferred to the field following previously standardized hardening procedures.
authors.affiliation: Plant Tissue Culture Laboratory, Department of Botany, University of Gorakhpur, 273 009, Gorakhpur, India.
authors.forename: J
authors.initials: J
authors.lastname: Mathur
authors.forename: P
authors.initials: P
authors.lastname: Singh Ahuja