title: Primary sequence, tissue specificity and mRNA expression of the Na(+),K (+) -ATPase β1 subunit in the European eel (Anguilla anguilla).
abstract: The entire amino acid coding sequence of the Na(+),K(+)-ATPase β1 isoform was cloned from the gill of the European eel (Anguilla anguilla) by a PCR based method. The amino acid sequence translated from the nucleotide sequence shared 61.4 and 56.2% homology respectively with previously published Na(+),K(+)-ATPase β1 isoform sequences from the clawed toad (Xenopus laevis) and the ray (Torpedo californica) an elasmobranch fish. The size of the Na(+),K(+)-ATPase β1 mRNA transcript in eel tissues was demonstrated to be 2.35 Kb. Detectable levels of Na(+),K(+)-ATPase β1 mRNA were found at some level in all tissues except liver and cardiac muscle. The level of branchial Na(+),K(+)-ATPase β1 mRNA was observed to increase after the adaptation of fresh water eels to normal or double concentration sea water.
authors.affiliation: School of Biological and Medical Sciences, Bute Medical Buildings, University of St Andrews, St Andrews, Fife, Scotland, UK, KY16 9TS.
authors.forename: C P
authors.initials: CP
authors.lastname: Cutler
authors.forename: I L
authors.initials: IL
authors.lastname: Sanders
authors.forename: N
authors.initials: N
authors.lastname: Hazon
authors.forename: G
authors.initials: G
authors.lastname: Cramb