title: Preparation of slides for microscopy from frozen tissue sections.
abstract: Freezing tissue is a convenient method for long-term storage. In addition, sectioning tissue under frozen conditions or embedded within a frozen matrix allows for stabilization of tissue during the sectioning process. This is advantageous for small and/or delicate tissue (e.g., rodent brains younger than postnatal day 7) and tissue that will be sectioned very thin (usually <150 μm).
authors.affiliation: Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: jbachma9@gmail.com.
authors.forename: Julia
authors.initials: J
authors.lastname: Bachman
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods*; D003451:Cryoprotective Agents; D007120:Immunochemistry / Q000379:methods; D008853:Microscopy / Q000379:methods*; D008867:Microtomy; D016610:Tissue Embedding / Q000379:methods
chemical_list: D003451:Cryoprotective Agents
keywords: Bembedding tissue sample; Cryoprotectant media; Cryostat section; Frozen tissue sections; Preparation of slides for immunochemistry