title: Electron paramagnetic resonance resolves effects of oxidative stress on muscle proteins.
abstract: We have used site-directed spin labeling and electron paramagnetic resonance (EPR) to explore the effects of oxidation on muscle function, with particular focus on the actin-myosin interaction. EPR measurements show that aging or oxidative modification causes a decrease in the fraction of myosins in the strong-binding state, which can be traced to the actin-binding cleft of the myosin catalytic domain.
authors.affiliation: 1Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN; 2Department of Chemistry and Geology, Minnesota State University, Mankato, Mankato, MN; and 3Department of Biology, University of Wisconsin, Lacrosse, Lacrosse, WI.
authors.forename: Rebecca J
authors.initials: RJ
authors.lastname: Moen
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Klein
authors.forename: David D
authors.initials: DD
authors.lastname: Thomas
mesh_terms: D000205:Actomyosin / Q000378:metabolism*; D000375:Aging / Q000378:metabolism; D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D008715:Methionine / Q000378:metabolism; D009218:Myosins / Q000378:metabolism; D018384:Oxidative Stress
chemical_list: D000205:Actomyosin; D008715:Methionine; D009218:Myosins