title: Gene transfection in primary stem-like cells of giant cell tumor of bone.
abstract: The neoplastic stem-like stromal cell of giant cell tumor of bone (GCT) survives for multiple passages in primary culture with a stable phenotype, and exhibits multipotent characteristics. The pathophysiology of this tumor has been studied through the primary culture of these cells. However, successful gene transfer of these cells has not been reported to date. In this short report, we describe the development of the first reported technique that results in efficient gene transfection in primary stem-like cells of GCT.
authors.affiliation: Department of Surgery, McMaster University, Hamilton, Ontario.
authors.forename: Shalini
authors.initials: S
authors.lastname: Singh
authors.forename: Isabella
authors.initials: I
authors.lastname: Mak
authors.forename: Patricia
authors.initials: P
authors.lastname: Power
authors.forename: Melissa
authors.initials: M
authors.lastname: Cunningham
authors.forename: Melissa
authors.initials: M
authors.lastname: Cunnigham
authors.forename: Robert
authors.initials: R
authors.lastname: Turcotte
authors.forename: Michelle
authors.initials: M
authors.lastname: Ghert
keywords: TWIST; gene; giant cell tumor; primary cells; transfection