title: Endophytic Streptomyces sp. Y3111 from traditional Chinese medicine produced antitubercular pluramycins.
abstract: As part of a search for antitubercular substances from natural sources, we screened a library of endophytic microbes (50 strains and 300 crude extracts in total) isolated from traditional Chinese medicines (TCMs) for growth inhibitory activity against Bacillus Calmette-Guérin (BCG). The crude extract of Streptomyces sp. strain Y3111, which was associated with the stems of Heracleum souliei, showed good anti-BCG activity with an MIC value of 12.5 μg/mL. Bioassay-guided isolation led to four new pluramycin-type compounds, heraclemycins A-D (1-4). Their structures were determined by different spectroscopic techniques including HRMSESI, 1D NMR, and 2D NMR. This is the first report of pluramycin analogues produced by TCM endophytic microbes as well as the first example of BCG-selective pluramycins. Heraclemycin C (3) showed selective antitubercular activity against BCG with a MIC value of 6.25 μg/mL and a potential new mode of action.
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Liu
authors.forename: Wael M
authors.initials: WM
authors.lastname: Abdel-Mageed
authors.forename: Biao
authors.initials: B
authors.lastname: Ren
authors.forename: Wenni
authors.initials: W
authors.lastname: He
authors.forename: Pei
authors.initials: P
authors.lastname: Huang
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Li
authors.forename: Krishna
authors.initials: K
authors.lastname: Bolla
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
authors.forename: Caixia
authors.initials: C
authors.lastname: Chen
authors.forename: Fuhang
authors.initials: F
authors.lastname: Song
authors.forename: Huanqin
authors.initials: H
authors.lastname: Dai
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Quinn
authors.forename: Tanja
authors.initials: T
authors.lastname: Grkovic
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zhang
authors.forename: Xueting
authors.initials: X
authors.lastname: Liu
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000617:Aminoglycosides / Q000378:metabolism*; D000995:Antitubercular Agents / Q000378:metabolism*; D060026:Endophytes / Q000302:isolation & purification / Q000378:metabolism*; D030002:Heracleum / Q000382:microbiology*; D008516:Medicine, Chinese Traditional; D008826:Microbial Sensitivity Tests; D009163:Mycobacterium bovis / Q000187:drug effects; D018547:Plant Stems / Q000382:microbiology; D013057:Spectrum Analysis; D013302:Streptomyces / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000617:Aminoglycosides; D000995:Antitubercular Agents; C003169:pluramycin