title: Vesicle extrusion through polycarbonate track-etched membranes using a hand-held mini-extruder.
abstract: The vesicle extrusion method is used for preparing small (e.g., 100 nm diameter) monodisperse (uniform-sized) vesicles. Polycarbonate track-etched membranes with pores generally ranging from 50 to 200 nm in diameter and a hand-held mini-extruder are used for such a procedure. Extrusion through small (100 nm in diameter or smaller) pores also ensures that the vesicle population is predominantly unilamellar.
authors.affiliation: Department of Molecular Biology, Howard Hughes Medical Institute, Massachusetts General Hospital, Boston, MA, USA.
authors.forename: Ting F
authors.initials: TF
authors.lastname: Zhu
authors.forename: Itay
authors.initials: I
authors.lastname: Budin
authors.forename: Jack W
authors.initials: JW
authors.lastname: Szostak
mesh_terms: D001671:Biochemistry / Q000295:instrumentation / Q000379:methods*; D008567:Membranes, Artificial; D011076:Polycarboxylate Cement; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: D008567:Membranes, Artificial; D011076:Polycarboxylate Cement; D053835:Unilamellar Liposomes; C005506:polycarbonate
keywords: Extrusion apparatus; Gas-tight syringes; Monodisperse vesicles; Polycarbonate track-etched membranes; Vesicle extrusion