title: Identification and characterization of MGEs and their insertion sites in thegorillagenome.
abstract: Recently published gorilla genome has offered an opportunity to study human evolution through variety of approaches. Mobile genetic elements (MGEs) insert non randomly in genome through mechanisms such as retrotransposition and may cause gene inactivation, transduction, regulation of gene expression and genome expansion. Here we report that majority of gorilla genome is occupied with MGEs (> 36%) with presence of LTRs and Non-LTRs such asAlus and L1s. Other types of MGEs such as MIRs, retrovirus like elements ERVs and DNA transposons are also found using repeatmasker and ELAN pipeline. The distribution is similar to Humans and Macaca genome. Using DNA Scanner we also scanned preinsertion loci for number of different properties such as DNA denaturation, energy measures, potential for protein interactions and sequence based features. We also predicted preinsertion loci with > 70% accuracy using a machine learning tool called insertion site finder (ISF) based upon support vector machines.
authors.affiliation: Department of Biotechnology; Jaypee Institute of Information Technology; Noida, UP India.
authors.forename: Kamal
authors.initials: K
authors.lastname: Rawal
authors.forename: Sangey
authors.initials: S
authors.lastname: Dorji
authors.forename: Amit
authors.initials: A
authors.lastname: Kumar
authors.forename: Anwesha
authors.initials: A
authors.lastname: Ganguly
authors.forename: Ankit Singh
authors.initials: AS
authors.lastname: Grewal
keywords: Alu; L1; LINEs; SINEs; mobile genetic elements; physiochemical properties; primates; truncation points