title: Single oral dose toxicity test of polycalcium, a mixed composition of polycan and calcium lactate-gluconate 1:9 (G/G) in SD rat.
abstract: The object of this study was to obtain acute oral toxicity information of Polycalcium, a mixed composition of Polycan and Calcium lactate-gluconate 1:9 (g/g), in Sprague-Dawely (SD) rats. In order to investigate the toxicity and identify target organs, Polycalcium were once orally administered to female and male SD rats at dose levels of 2000, 1000, 500 and 0 (control) mg/kg body weights. The mortality, changes on body weight and clinical signs were monitored during 14 days after treatment with gross observation, changes on the organ weights and histopathology of principle organs and treatment sites based on the recommendation of KFDA Guidelines [2009-116, 2009]. As the results of single oral treatment of Polycalcium, no treatment related mortalities were observed within 14 days after end of treatment up to 2000 mg/kg, the limited dosage of rodents in the both genders. In addition, no Polycalcium treatment related changes on the body and organ weights, clinical signs, necropsy and histopathological findings were detected. The results obtained in this study suggest that the Polycalcium is non-toxic in rats. The LD50 and approximate LD in rats after single oral dose of Polycalcium were considered over 2000 mg/kg in both female and male, respectively.
authors.affiliation: Glucan Corp. Research Institute, Marine Biotechnology Center, Street 100, Gwaebup-dong, Sasang-gu, Busan, Republic of Korea.
authors.forename: Joo-Wan
authors.initials: JW
authors.lastname: Kim
authors.forename: Jae-Suk
authors.initials: JS
authors.lastname: Choi
authors.forename: Yu-Mi
authors.initials: YM
authors.lastname: Ha
authors.forename: In Soon
authors.initials: IS
authors.lastname: Choi
authors.forename: Ki-Young
authors.initials: KY
authors.lastname: Kim
authors.forename: Hyung-rae
authors.initials: HR
authors.lastname: Cho
authors.forename: Chae-hun
authors.initials: CH
authors.lastname: Rha
authors.forename: Sae-Kwang
authors.initials: SK
authors.lastname: Ku
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D017610:Calcium Compounds / Q000008:administration & dosage / Q000633:toxicity*; D002125:Calcium Gluconate / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D007773:Lactates / Q000008:administration & dosage / Q000633:toxicity*; D007928:Lethal Dose 50; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D018675:Toxicity Tests; D047071:beta-Glucans / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D017610:Calcium Compounds; D007773:Lactates; D047071:beta-Glucans; C110051:calcium lactate; D002125:Calcium Gluconate