title: Anti-proliferative effect of a compound isolated from Cassia auriculata against human colon cancer cell line HCT 15.
abstract: The compound was isolated from leaves of Cassia auriculata and its structure was characterized using high-performance liquid chromatography (HPLC), liquid chromatography mass spectrometry (LC-MS), UV-vis spectroscopy (UV-vis), fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy (NMR). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, cytotoxicity, nuclear morphology and lactate dehydrogenase assay of isolated compound was tested against human colon cancer cell line HCT 15. The isolated compound, 4-(4-chlorobenzyl)-2,3,4,5,6,7-hexahydro-7-(2-ethoxyphenyl)benzo[h][1,4,7]triazecin-8(1H)-one at 25μg/ml concentration and by 48h showed 50% inhibition of human colon cancer cells (HCT 15). The results suggest that isolated compound from C. auriculata has potential to prevent colon cancer cell line.
authors.affiliation: Department of Animal Health and Management, Alagappa University, Karaikudi 630 003, India.
authors.forename: M
authors.initials: M
authors.lastname: Esakkirajan
authors.affiliation: Department of Animal Health and Management, Alagappa University, Karaikudi 630 003, India. Electronic address: prabhunm71@gmail.com.
authors.forename: N M
authors.initials: NM
authors.lastname: Prabhu
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, India.
authors.forename: C
authors.initials: C
authors.lastname: Arulvasu
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, India.
authors.forename: M
authors.initials: M
authors.lastname: Beulaja
authors.affiliation: Department of Animal Health and Management, Alagappa University, Karaikudi 630 003, India. Electronic address: manikandanramar@yahoo.co.in.
authors.forename: R
authors.initials: R
authors.lastname: Manikandan
authors.affiliation: Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613 401, India.
authors.forename: R
authors.initials: R
authors.lastname: Thiagarajan
authors.affiliation: Centre for Ocean Research, Sathyabama University, Chennai 600 119, India.
authors.forename: K
authors.initials: K
authors.lastname: Govindaraju
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, India.
authors.forename: D
authors.initials: D
authors.lastname: Prabhu
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, India.
authors.forename: D
authors.initials: D
authors.lastname: Dinesh
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, India.
authors.forename: G
authors.initials: G
authors.lastname: Babu
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, India.
authors.forename: G
authors.initials: G
authors.lastname: Dhanasekaran
mesh_terms: D016588:Anticarcinogenic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002366:Cassia / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000517:prevention & control*; D006801:Humans
chemical_list: D016588:Anticarcinogenic Agents; D000972:Antineoplastic Agents, Phytogenic
keywords: Anti-proliferative; Cassia auriculata; HCT 15 cell line