title: Reverse-phase HPLC analysis and purification of small molecules.
abstract: Reversed phase high-performance liquid chromatography (HPLC) is utilized for the separation of molecules due to their polarity in order to quantify, identify, and/or purify various samples such as those from serum, human and animal tissues, drugs, and foods. The following protocols are for extracting carotenoids from samples and subsequent HPLC analysis. If you are interested in another compound for HPLC analysis, Sigma-Aldrich® has a wonderful online resource for multiple adaptations to the HPLC protocol for the analysis of hundreds of compounds (see References).
authors.affiliation: Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD, USA. Electronic address: kirstieadams26@yahoo.com.
authors.forename: Kirstie
authors.initials: K
authors.lastname: Canene-Adams
mesh_terms: D000818:Animals; D002338:Carotenoids / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D006801:Humans
chemical_list: D002338:Carotenoids
keywords: Extraction of carotenoids; High-performance liquid chromatography (HPLC); Purification of small molecules; Reverse-phase HPLC-PDA system; Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis