title: Analgesic effects of Huwentoxin-IV on animal models of inflammatory and neuropathic pain.
abstract: Huwentoxin-IV (HWTX-IV), a peptide with 35 amino acid residues, was discovered in the venom of spider Ornithoctonus huwena. The peptide had an inhibitory effect on a tetrodotoxin-sensitive (TTX-S) sodium channel with highly sensitive to Nav1.7, an attractive target for pain release therapy. In this study we further demonstrated the analgesic effects of HWTX-IV using mouse and rat as an inflammatory pain model and/or a neuropathic pain models. In the both cases, the analgesic effects of the peptide were dose-dependent, and statistically significant. In the inflammatory model, 100 µg/kg of HWTX-IV produced an efficient reversal of hyperalgesia up to 63.6% after injection of formalin in rats with the efficiency equivalent to that of morphine at 50 µg/kg, and 200 µg/kg of HWTX-IV produced protective effect up to 55.6% after injection of acetic acid with the efficiency equivalent to that of morphine at 100 µg/kg. In the spinal nerve model, the peptide produced the longer and higher reversal effect on allodynia than Mexiletine. These results demonstrated that HWTX-IV released efficiently the acute inflammatory pain and chronic neuropathic pain in these animals, suggesting that HWTX-IV was a potential and efficient candidate for further clinical drug development against inflammatory and neuropathic pain.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wu
authors.forename: Dongfang
authors.initials: D
authors.lastname: Tang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Xun
authors.forename: Lichao
authors.initials: L
authors.lastname: Liu
authors.forename: Xianlei
authors.initials: X
authors.lastname: Li
authors.forename: Dongsong
authors.initials: D
authors.lastname: Nie
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiang
authors.forename: Jianming
authors.initials: J
authors.lastname: Yi
authors.affiliation: College of Chemistry and Chemical Engineering Hunan Institute of Science and Technology Yueyang, 414006, China. jizu_yi@yahoo.com.
authors.forename: Jizu
authors.initials: J
authors.lastname: Yi
mesh_terms: D019342:Acetic Acid / Q000009:adverse effects; D000700:Analgesics / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D004195:Disease Models, Animal; D005557:Formaldehyde / Q000009:adverse effects; D007249:Inflammation / Q000188:drug therapy; D051379:Mice; D009437:Neuralgia / Q000139:chemically induced / Q000188:drug therapy*; D051381:Rats; D013111:Spider Venoms / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000700:Analgesics; D013111:Spider Venoms; D005557:Formaldehyde; D019342:Acetic Acid