title: The promoter of barley trypsin-inhibitor BTI-CMe, discriminates between wheat and barley endosperm protoplasts in transient expression assays.
abstract: Several promoter fragments from the barley gene coding for trypsin inhibitor, BTI-CMe, have been fused to the β-glucuronidase (GUS) reporter gene and these chimeric constructs used for transient expression in protoplasts. Transfection of developing endosperm protoplasts from barley (cv Bomi) show a maximum GUS expression of about 50% of that driven by the cauliflower mosaic virus 35S promoter, while in wheat endosperm protoplasts expression is less than 10%. No significant expression is found in transfected leaf protoplasts from barley, wheat or tobacco (<2% of the 35S control). All the information required for endosperm and barley specificity is present in the 343 bp proximal to the translation initiation site.
authors.affiliation: Centro Nacional de Biotecnología, CSIC, 28049, Madrid, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Díaz
authors.forename: J
authors.initials: J
authors.lastname: Royo
authors.forename: P
authors.initials: P
authors.lastname: Carbonero