title: Encapsulation and crystallization of Prussian blue nanoparticles by cowpea chlorotic mottle virus capsids.
abstract: Cowpea chlorotic mottle virus (CCMV) capsids were used to encapsulate Prussian blue (PB) particles based on electrostatic interaction. A negatively-charged metal complex, hexacyanoferrate (III), was entrapped inside the capsids through the disassembly/reassembly process under a pH change from 7.5 to 5.2. The loaded capsids reacted with a second Fe(II) to fabricate PB particles. The synthesis of PB in CCMV capsids was confirmed by a unique colour transition at 710 nm and by size-exclusion FPLC. Transmission electron microscopy images of PB-CCMV biohybrids presented discrete spherical particles with a relatively homogeneous size. Dynamic light scattering of PB-CCMV showed two peaks of 29.2 ± 1.7 nm corresponding to triangulation number T = 3 particles, and 17.5 ± 1.2 nm of pseudo T = 2 particles. The encapsulation and crystallization of PB in CCMV provided an efficient method for the self-organization of bimetallic nanoparticles.
authors.affiliation: Department of Chemical and Biochemical Engineering, Chosun University, #375 Seosuk-dong, Dong-gu, Gwangju, 501-759, Republic of Korea, yuanzheng.wu@hogmail.com.
authors.forename: Yuanzheng
authors.initials: Y
authors.lastname: Wu
authors.forename: Hetong
authors.initials: H
authors.lastname: Yang
authors.forename: Hyun-Jae
authors.initials: HJ
authors.lastname: Shin
mesh_terms: D017795:Bromovirus / Q000737:chemistry*; D002213:Capsid / Q000737:chemistry*; D055598:Chemical Phenomena; D002850:Chromatography, Gel; D003116:Color; D003460:Crystallization; D005292:Ferricyanides / Q000378:metabolism; D005295:Ferrocyanides / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles; D055672:Static Electricity
chemical_list: D005292:Ferricyanides; D005295:Ferrocyanides; C007931:hexacyanoferrate III; C000170:ferric ferrocyanide