title: Plant regeneration from mesophyll protoplasts of Moricandia arvensis.
abstract: Moricandia arvensis is of interest as it is a dicotyledonous species which has C3 - C4 intermediate photosynthesis, a mechanism which results in enhanced recapture of photorespired CO2. Leaves from cultured shoot tips were used as a source for mesophyll cell protoplasts. Approximately 1% of the protoplasts which survived the first few days of culture produced calli. On a suitable regeneration medium, 30-60% of the calli regenerated one or more shoots. From among the regenerating shoots eight were selected, transferred to soil and grown to flowering in the glasshouse; all were fertile. The development of a protoplast regeneration system provides the opportunity to use transformation and somaclonal variation as tools in the genetic analysis of the C3-C4 character in this species.
authors.affiliation: Brassica and Oilseed Research Department, Cambridge Laboratory, John Innes Centre, Colney Lane, NR4 7UJ, Norwich, UK.
authors.forename: T
authors.initials: T
authors.lastname: Murata
authors.forename: R J
authors.initials: RJ
authors.lastname: Mathias