title: Investigating the transport dynamics of anthocyanins from unprocessed fruit and processed fruit juice from sour cherry (Prunus cerasus L.) across intestinal epithelial cells.
abstract: Anthocyanins can contribute to human health through preventing a variety of diseases. The uptake of these compounds from food and the parameters determining uptake efficiency within the human body are still poorly understood. Here we have employed a Caco-2 cell based system to investigate the transport of key antioxidant food components from sour cherry (Prunus cerasus L.) across the intestinal epithelial barrier. Anthocyanins and (-)-epicatechin were supplied in three contrasting matrices: fruit, processed fruit cherry juice, and polyphenolic fractions obtained by solid-phase extraction. Results show that both compound types behave differently. Fruit or juice matrices display comparable transport across the epithelial cell layer. The juice supplements sucrose and citric acid, which are regularly added to processed foods, have a positive effect on stability and transport. Polyphenolic fractions display a lower transport efficiency, relative to that of the fruit or juice, indicating the importance of food matrix components for intestinal absorption of polyphenols.
authors.affiliation: Faculty of Chemical and Metallurgical Engineering, Food Engineering Department, Istanbul Technical University , Maslak 34469, Istanbul, Turkey.
authors.forename: Gamze
authors.initials: G
authors.lastname: Toydemir
authors.forename: Dilek
authors.initials: D
authors.lastname: Boyacioglu
authors.forename: Esra
authors.initials: E
authors.lastname: Capanoglu
authors.forename: Ingrid M
authors.initials: IM
authors.lastname: van der Meer
authors.forename: Monic M M
authors.initials: MM
authors.lastname: Tomassen
authors.forename: Robert D
authors.initials: RD
authors.lastname: Hall
authors.forename: Jurriaan J
authors.initials: JJ
authors.lastname: Mes
authors.forename: Jules
authors.initials: J
authors.lastname: Beekwilder
mesh_terms: D000872:Anthocyanins / Q000493:pharmacokinetics*; D001628:Beverages; D001682:Biological Availability; D018938:Caco-2 Cells; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects; D005511:Food Handling; D005638:Fruit / Q000737:chemistry; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000166:cytology / Q000187:drug effects; D027861:Prunus / Q000737:chemistry*; D052616:Solid Phase Extraction
chemical_list: D000872:Anthocyanins; D003470:Culture Media