title: Gelatin nanostructured lipid carriers-mediated intranasal delivery of basic fibroblast growth factor enhances functional recovery in hemiparkinsonian rats.
abstract: Lipid nanoparticles with solid matrix have been given increasing attention due to their biodegradable status and ability to entrap a variety of biologically active compounds. In this study, new phospholipid-based gelatin nanoparticles encapsulating basic fibroblast growth factor (bFGF) were developed to target the brain via nasal administration. Treatment effects were assessed by quantifying rotational behavior, monoamine neurotransmitter levels and tyrosine hydroxylase expression in 6-hydroxydopamine induced hemiparkinsonian rats. The gelatin nanostructured lipid carriers (GNLs) were prepared by a water-in-water emulsion method and then freeze-dried. The GNLs possessed better profile than gelatin nanoparticles (GNs), with particle size 143±1.14nm and Zeta potential -38.2±1.2mV. The intranasal GNLs efficiently enriched exogenous bFGF in olfactory bulb and striatum without adverse impact on the integrity of nasal mucosa and showed obvious therapeutic effects on hemiparkinsonian rats. Thus, GNLs are attractive carriers for nose-to-brain drug delivery, especially for unstable macromolecular drugs such as bFGF.
  
  
  BACKGROUND
  This team of authors reports the development of phospholipid-based gelatin nanoparticles encapsulating basic fibroblast growth factor to target the brain via intranasal administration. A rat model of hemiparkinsonism was applied demonstrating a good safety profile and an obvious therapeutic effect.
authors.affiliation: Pharmacy School, Wenzhou Medical University, Wenzhou City, Zhejiang Province, China; St. Christopher's Hospital for Children/Drexel University College of Medicine, Philadelphia, PA, USA.
authors.forename: Ying-Zheng
authors.initials: YZ
authors.lastname: Zhao
authors.affiliation: Pharmacy Department, Hangzhou Xiasha Hospital, Hangzhou, Zhejiang Province, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Li
authors.affiliation: Pharmacy School, Wenzhou Medical University, Wenzhou City, Zhejiang Province, China.
authors.forename: Cui-Tao
authors.initials: CT
authors.lastname: Lu
authors.affiliation: Pharmacy School, Wenzhou Medical University, Wenzhou City, Zhejiang Province, China.
authors.forename: Min
authors.initials: M
authors.lastname: Lin
authors.affiliation: Pharmacy School, Wenzhou Medical University, Wenzhou City, Zhejiang Province, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Chen
authors.affiliation: Biopharmaceutical R&D Center of Jinan University, Guangzhou, Guangdong Province, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Xiang
authors.affiliation: Pharmacy School, Wenzhou Medical University, Wenzhou City, Zhejiang Province, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Pharmacy School, Wenzhou Medical University, Wenzhou City, Zhejiang Province, China.
authors.forename: Rong-Rong
authors.initials: RR
authors.lastname: Jin
authors.affiliation: Pharmacy School, Wenzhou Medical University, Wenzhou City, Zhejiang Province, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Pharmacy School, Wenzhou Medical University, Wenzhou City, Zhejiang Province, China.
authors.forename: Xiao-Tong
authors.initials: XT
authors.lastname: Shen
authors.affiliation: Pharmacy School, Wenzhou Medical University, Wenzhou City, Zhejiang Province, China. Electronic address: rarayier@gmail.com.
authors.forename: Xiao-Kun
authors.initials: XK
authors.lastname: Li
authors.affiliation: Departments of Pediatrics and Anatomical Sciences and Neurobiology, University of Louisville School of Medicine, Louisville, KY, USA. Electronic address: j0cai002@louisville.edu.
authors.forename: Jun
authors.initials: J
authors.lastname: Cai
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D003342:Corpus Striatum / Q000503:physiopathology; D004337:Drug Carriers / Q000737:chemistry / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000494:pharmacology*; D005780:Gelatin / Q000737:chemistry / Q000494:pharmacology*; D008055:Lipids / Q000737:chemistry / Q000494:pharmacology*; D053758:Nanoparticles; D009830:Olfactory Bulb / Q000503:physiopathology; D010302:Parkinson Disease, Secondary / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D051381:Rats
chemical_list: D004337:Drug Carriers; D008055:Lipids; D016222:Fibroblast Growth Factor 2; D005780:Gelatin
keywords: Gelatin nanostructured lipid carriers; Hemiparkinsonian; Nose-to-brain drug delivery; bFGF