title: Fluorescence discrimination of cancer from inflammation by molecular response to COX-2 enzymes.
abstract: Accurate identification of cancer from inflammation and normal tissue in a rapid, sensitive, and quantitative fashion is important for cancer diagnosis and resection during surgery. Here we report the use of cyclooxygenase-2 as a marker for identification of cancer from inflammation and the design of a novel smart COX-2-specific fluorogenic probe (NANQ-IMC6). The probe's fluorescence is "turned on" in both inflammations and cancers where COX-2 is overexpressed. Intriguingly, the fluorescent emission is quite different at these two sites with different expression level of COX-2. Hence, NANQ-IMC6 can not only distinguish normal cells/tissues from cancer cells/tissues but also distinguish the latter from sites of inflammation lesions by the different fluorescence recognition of NANQ-IMC6 for COX-2 enzymes. Following spraying with the NANQ-IMC6 solution, cancerous tissue, inflamed tissues, and normal tissues can be accurately discriminated in vivo by the unaided eye using a hand-held ultraviolet lamp emitting at 365 nm. So the probe may have potential application varying from cancer inflammation diagnosis to guiding tumor resection during surgery.
authors.affiliation: State Key Laboratory of Fine Chemicals, ‡School of Life Science and Biotechnology, and §School of Pharmaceutical Science and Technology, Dalian University of Technology , 2 Linggong Road, Hi-tech Zone, Dalian 116024, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Jiangli
authors.initials: J
authors.lastname: Fan
authors.forename: Jingyun
authors.initials: J
authors.lastname: Wang
authors.forename: Bairui
authors.initials: B
authors.lastname: Dou
authors.forename: Fan
authors.initials: F
authors.lastname: Zhou
authors.forename: Jianfang
authors.initials: J
authors.lastname: Cao
authors.forename: Junle
authors.initials: J
authors.lastname: Qu
authors.forename: Zhi
authors.initials: Z
authors.lastname: Cao
authors.forename: Weijie
authors.initials: W
authors.lastname: Zhao
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Peng
mesh_terms: D051546:Cyclooxygenase 2 / Q000737:chemistry / Q000378:metabolism*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D008958:Models, Molecular; D015394:Molecular Structure; D009369:Neoplasms / Q000201:enzymology / Q000378:metabolism*; D011789:Quantum Theory; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D051546:Cyclooxygenase 2