title: Inhibition of endocytic vesicle fusion by Plk1-mediated phosphorylation of vimentin during mitosis.
abstract: Endocytic vesicle fusion is inhibited during mitosis, but the molecular pathways that mediate the inhibition remain unclear. Here we uncovered an essential role of Polo-like kinase 1 (Plk1) in this mechanism. Phosphoproteomic analysis revealed that Plk1 phosphorylates the intermediate filament protein vimentin on Ser459, which is dispensable for its filament formation but is necessary for the inhibition of endocytic vesicle fusion in mitosis. Furthermore, this mechanism is required for integrin trafficking toward the cleavage furrow during cytokinesis. Our results thus identify a novel mechanism for fusion inhibition in mitosis and implicate its role in vesicle trafficking after anaphase onset.
authors.affiliation: Department of Cell Biology; Institute for Virus Research; Kyoto University; Sakyo-ku, Kyoto, Japan; Department of Mammalian Regulatory Network; Graduate School of Biostudies; Kyoto University; Sakyo-ku, Kyoto, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Ikawa
authors.affiliation: Department of Molecular & Cellular BioAnalysis; Graduate School of Pharmaceutical Sciences; Kyoto University; Sakyo-ku, Kyoto, Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Satou
authors.affiliation: Department of Cell Biology; Institute for Virus Research; Kyoto University; Sakyo-ku, Kyoto, Japan; Department of Mammalian Regulatory Network; Graduate School of Biostudies; Kyoto University; Sakyo-ku, Kyoto, Japan.
authors.forename: Mitsuko
authors.initials: M
authors.lastname: Fukuhara
authors.affiliation: Department of Cell Biology; Institute for Virus Research; Kyoto University; Sakyo-ku, Kyoto, Japan; Department of Mammalian Regulatory Network; Graduate School of Biostudies; Kyoto University; Sakyo-ku, Kyoto, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Matsumura
authors.affiliation: Department of Molecular & Cellular BioAnalysis; Graduate School of Pharmaceutical Sciences; Kyoto University; Sakyo-ku, Kyoto, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Sugiyama
authors.affiliation: Division of Biochemistry; Aichi Cancer Center Research Institute; Chikusa-ku, Nagoya, Japan.
authors.forename: Hidemasa
authors.initials: H
authors.lastname: Goto
authors.affiliation: Department of Developmental Biology and Neurosciences; Graduate School of Life Sciences; Tohoku University; Sendai, Miyagi, Japan.
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Fukuda
authors.affiliation: Division of Biochemistry; Aichi Cancer Center Research Institute; Chikusa-ku, Nagoya, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Inagaki
authors.affiliation: Department of Molecular & Cellular BioAnalysis; Graduate School of Pharmaceutical Sciences; Kyoto University; Sakyo-ku, Kyoto, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ishihama
authors.affiliation: Department of Cell Biology; Institute for Virus Research; Kyoto University; Sakyo-ku, Kyoto, Japan; Department of Mammalian Regulatory Network; Graduate School of Biostudies; Kyoto University; Sakyo-ku, Kyoto, Japan.
authors.forename: Fumiko
authors.initials: F
authors.lastname: Toyoshima
mesh_terms: D000705:Anaphase / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D048749:Cytokinesis; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis / Q000235:genetics*; D010766:Phosphorylation / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D022161:Transport Vesicles / Q000235:genetics* / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism*; D000097869:Polo-Like Kinase 1
chemical_list: D018797:Cell Cycle Proteins; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D014746:Vimentin; D000097869:Polo-Like Kinase 1
keywords: Plk1; endosome; mitosis; vesicle fusion; vimentin