title: Exposure to leachate from municipal battery recycling site: implication as key inhibitor of steroidogenic enzymes and risk factor of prostate damage in rats.
abstract: Few or no studies have measured the effect of short- and long-term exposure to industrial leachate. Mature male Wistar strain albino rats (175-220 g) underwent sub-chronic exposure to leachate from the Elewi Odo municipal battery recycling site (EOMABRL) via oral administration for a period of 60 days at different doses (20%, 40%, 60%, 80%, and 100%) per kilogram of body weight to evaluate the toxic effects of the leachate on male reproductive function using steroidogenic enzymes and biomarkers of prostate damage. Control groups were treated equally but were given distilled water instead of the leachate. After the treatment periods, results showed that the treatment induced systemic toxicity at the doses tested by causing a significant (p<0.05) loss in absolute body weight and decline in growth rate. There was a marked significant decrease (p<0.05) in testicular activities of Δ(5)-3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase. Conversely, the activity of prostatic acid phosphatase, a key marker enzyme for prostrate damage was significantly (p<0.05) elevated in the treated rats. Similarly, the administration of EOMABRL significantly (p<0.05) exacerbated the activity of total acid phosphatase with concomitant increase in the activity of prostatic alkaline phosphatase. These findings conclude that exposure to leachate from a battery recycling site induces sub-chronic testicular toxicity by inhibiting key steroidogenic enzymes and activating key markers linked with prostate damage/cancer in rats.
authors.forename: Jacob K
authors.initials: JK
authors.lastname: Akintunde
authors.forename: G
authors.initials: G
authors.lastname: Oboh
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000032:analysis*; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood*; D001835:Body Weight / Q000187:drug effects; D008297:Male; D019216:Metals, Heavy / Q000032:analysis; D009549:Nigeria; D010084:Oxidation-Reduction; D011471:Prostatic Neoplasms / Q000139:chemically induced* / Q000201:enzymology / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000737:chemistry / Q000187:drug effects; D036401:Toxicity Tests, Chronic; D014166:Transferases / Q000097:blood; D014874:Water Pollutants, Chemical / Q000737:chemistry* / Q000633:toxicity*
chemical_list: D014408:Biomarkers, Tumor; D019216:Metals, Heavy; D014874:Water Pollutants, Chemical; D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase; D014166:Transferases; D000469:Alkaline Phosphatase