title: The histone deacetylase inhibitor Scriptaid improves in vitro developmental competence of ovine somatic cell nuclear transferred embryos.
abstract: Although the success rate of sheep cloning remains extremely low, using a histone deacetylase (HDAC) inhibitor to increase histone acetylation in SCNT embryos has significantly enhanced developmental competence in several species. The objective was to determine whether HDAC inhibitors trichostatin A (TSA) and the novel inhibitor Scriptaid enhance cloning efficiency in sheep cumulus cell (passage 2) reconstructed embryos. In this study, 0.2 μmol/L Scriptaid yielded a high blastocyst development rate, almost twice that of the untreated group (25/103 [24.3%] vs. 12/101 [11.9%]; P < 0.05). Furthermore, 0.2 μmol/L Scriptaid was more effective than 0.05 μmol/L TSA in terms of the blastocyst percentage for cloned ovine embryos in vitro (17/66 [25.7%] vs. 11/65 [16.8%]; P < 0.05). Furthermore, treatment with Scriptaid increased acetylation (compared with the Control, P < 0.05) at lysine residue 12 of histone H4 (acH4K12) and lysine residue 9 of histone H3 (acH3K9) in one-, two-, four-, and eight-cell stages, as well as blastocyst stages, in cloned embryos. In conclusion, Scriptaid was more effective than TSA to enhance in vitro developmental competence in ovine SCNT embryos; furthermore, Scriptaid improved epigenetic status.
authors.affiliation: College of Animal Science and Technology, Agricultural University of Hebei, Baoding, Hebei, P.R. China; The Research Center of Cattle and Sheep Embryonic Technique of Hebei Province, Baoding, Hebei, P.R. China.
authors.forename: Bing-Qiang
authors.initials: BQ
authors.lastname: Wen
authors.affiliation: Reproductive Medical Center, First Hospital of Hebei Medical University, Shijiazhuang, Hebei, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Agricultural University of Hebei, Baoding, Hebei, P.R. China; The Research Center of Cattle and Sheep Embryonic Technique of Hebei Province, Baoding, Hebei, P.R. China. Electronic address: lijunjie816@163.com.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Agricultural University of Hebei, Baoding, Hebei, P.R. China; The Research Center of Cattle and Sheep Embryonic Technique of Hebei Province, Baoding, Hebei, P.R. China.
authors.forename: Shu-Jun
authors.initials: SJ
authors.lastname: Tian
authors.affiliation: College of Animal Science and Technology, Agricultural University of Hebei, Baoding, Hebei, P.R. China; The Research Center of Cattle and Sheep Embryonic Technique of Hebei Province, Baoding, Hebei, P.R. China.
authors.forename: Shu-Chun
authors.initials: SC
authors.lastname: Sun
authors.affiliation: College of Animal Science and Technology, Agricultural University of Hebei, Baoding, Hebei, P.R. China; The Research Center of Cattle and Sheep Embryonic Technique of Hebei Province, Baoding, Hebei, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Qi
authors.affiliation: College of Animal Science and Technology, Agricultural University of Hebei, Baoding, Hebei, P.R. China; The Research Center of Cattle and Sheep Embryonic Technique of Hebei Province, Baoding, Hebei, P.R. China.
authors.forename: Wen-Tao
authors.initials: WT
authors.lastname: Cai
authors.affiliation: College of Animal Science and Technology, Agricultural University of Hebei, Baoding, Hebei, P.R. China; The Research Center of Cattle and Sheep Embryonic Technique of Hebei Province, Baoding, Hebei, P.R. China.
authors.forename: Qing-Ling
authors.initials: QL
authors.lastname: Chang
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000187:drug effects*; D047108:Embryonic Development / Q000187:drug effects*; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006898:Hydroxylamines / Q000494:pharmacology*; D053652:Nuclear Transfer Techniques / Q000662:veterinary*; D011804:Quinolines / Q000494:pharmacology*; D012756:Sheep / Q000196:embryology*
chemical_list: D056572:Histone Deacetylase Inhibitors; D006898:Hydroxylamines; D011804:Quinolines; C410733:scriptaid
keywords: Developmental competence; Histone acetylation; Nuclear transfer; Ovine embryo; Scriptaid; TSA