title: Dynamics of cytomegalovirus populations harbouring mutations in genes UL54 and UL97 in a haematopoietic stem cell transplant recipient.
abstract: We characterised by pyrosequencing, the dynamics of cytomegalovirus populations harbouring mutations A594V in gene UL97 and A834P and Q578H in gene UL54 in a haematopoietic stem cell transplant recipient. Unexpected re-emergence of A594V and decrease of A834P under CMX001 were shown to depend on both the selection pressure exerted by the antiviral treatments and the immune response.
authors.affiliation: Univ Paris Diderot, Pres Sorbone Paris Cité, Inserm U941, APHP, Microbiology Laboratory, Hôpital Saint-Louis, Paris, France; National Reference Center for CMV,Hospital Saint-Louis, Paris, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Schnepf
authors.forename: Nathalie
authors.initials: N
authors.lastname: Dhédin
authors.forename: Séverine
authors.initials: S
authors.lastname: Mercier-Delarue
authors.forename: Annalisa
authors.initials: A
authors.lastname: Andreoli
authors.forename: Anne-Claire
authors.initials: AC
authors.lastname: Mamez
authors.forename: Christelle
authors.initials: C
authors.lastname: Ferry
authors.forename: Claire
authors.initials: C
authors.lastname: Deback
authors.forename: Patricia
authors.initials: P
authors.lastname: Ribaud
authors.forename: Marie
authors.initials: M
authors.lastname: Robin
authors.forename: Gérard
authors.initials: G
authors.lastname: Socié
authors.forename: François
authors.initials: F
authors.lastname: Simon
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Mazeron
mesh_terms: D000328:Adult; D000998:Antiviral Agents / Q000627:therapeutic use; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000188:drug therapy / Q000821:virology*; D024882:Drug Resistance, Viral / Q000235:genetics; D005260:Female; D005814:Genes, Viral; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D009154:Mutation; D055815:Young Adult
chemical_list: D000998:Antiviral Agents
keywords: CDV; CMV; CTSQ; Cytomegalovirus; FOS; GCV; HSCT; Kinetics; Mutation; PSQ; Resistance; Stem cell transplantation; cidofovir; cytomegalovirus; direct dideoxy chain termination sequencing; foscarnet; ganciclovir; haematopoietic stem cell transplant; pyrosequencing; vGCV; valganciclovir