title: Characterization of three hormone mutants of Nicotiana plumbaginifolia: evidence for a common ABA deficiency.
abstract: Various auxin-resistant Nicotiana plumbaginifolia mutants have already been isolated, including 1217 which shows cross-resistance to paclobutrazol. Recently, a cytokinin-resistant mutant, CKR1, has been characterized and has been shown to be affected in abscisic acid (ABA) biosynthesis. We have isolated a new mutant, Esg152, which was selected on the basis of its early germination. In each of these mutants, resistance is due to a recessive nuclear mutation at a single locus. Complementation analysis indicated that mutants I217, CKR1 and Esg152 belong to the same complementation group. They have a similar phenotype, which includes a reduction in seed dormancy and an increased tendency to wilt. These mutants display an increased auxin tolerance and enhanced root formation when leaf or hypocotyl sections are cultivated on auxin. By immunoenzymatic methods, we show that the endogenous levels of ABA are significantly lower than in the wild-type. We have assigned the symbol aba1 to the recessive alleles of the locus affected in the three mutants. The complexity of hormonal interactions is discussed briefly emerging from a consideration of this class of mutants.
authors.affiliation: Laboratoire de Biologie Cellulaire, INRA Versailles, F-78026, Versailles cedex, France.
authors.forename: P
authors.initials: P
authors.lastname: Rousselin
authors.forename: Y
authors.initials: Y
authors.lastname: Kraepiel
authors.forename: R
authors.initials: R
authors.lastname: Maldiney
authors.forename: E
authors.initials: E
authors.lastname: Miginiac
authors.forename: M
authors.initials: M
authors.lastname: Caboche