title: The small GTPase Rab5 homologue Ypt5 regulates cell morphology, sexual development, ion-stress response and vacuolar formation in fission yeast.
abstract: Inner-membrane transport is critical to cell function. Rab family GTPases play an important role in vesicle transport. In mammalian cells, Rab5 is reported to be involved in the regulation of endosome formation, phagocytosis and chromosome alignment. Here, we examined the role of the fission yeast Rab5 homologue Ypt5 using a point mutant allele. Mutant cells displayed abnormal cell morphology, mating, sporulation, endocytosis, vacuole fusion and responses to ion stress. Our data strongly suggest that fission yeast Rab5 is involved in the regulation of various types of cellular functions.
authors.affiliation: Graduate School of Science, Kobe University, 1-1 Rokkodai-cho Nada, Kobe 657-8501, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Tsukamoto
authors.forename: Chisako
authors.initials: C
authors.lastname: Katayama
authors.forename: Miki
authors.initials: M
authors.lastname: Shinohara
authors.forename: Akira
authors.initials: A
authors.lastname: Shinohara
authors.forename: Shohei
authors.initials: S
authors.lastname: Maekawa
authors.forename: Masaaki
authors.initials: M
authors.lastname: Miyamoto
mesh_terms: D004705:Endocytosis / Q000235:genetics; D049770:Genes, Mating Type, Fungal; D008561:Membrane Fusion; D017354:Point Mutation; D012568:Schizosaccharomyces / Q000166:cytology* / Q000235:genetics / Q000254:growth & development*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D013172:Spores, Fungal / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D013312:Stress, Physiological / Q000235:genetics; D014617:Vacuoles / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D029702:Schizosaccharomyces pombe Proteins; C083256:ypt5 protein, S pombe; D020691:rab GTP-Binding Proteins
keywords: A small GTPase; Fission yeast; Rab5