title: A 2-nt RNA enhancer on exon 11 promotes exon 11 inclusion of the Ron proto-oncogene.
abstract: Ron is a human receptor for the macrophage-stimulating protein (MSP). Exon 11 skipping of Ron pre-mRNA produces the Ron∆165 protein that has a deletion of a 49 amino acid region in the β-chain extracellular domain. Ron∆165 is constitutively active even in the absence of its ligand. Through stepwise deletion analysis, we identified a 2-nt RNA enhancer, which is located 74 nt upstream from the 5' splice site of exon 11, for exon 11 inclusion. Through double-base and single-base substitution analysis of the 2-nt RNA, we demonstrated that the GA, CC, UG and AC dinucleotides on exon 11, in addition to the wild-type AG sequence, function as enhancers for exon 11 inclusion of the Ron pre-mRNA.
authors.affiliation: School of life Sciences, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea.
authors.forename: Heegyum
authors.initials: H
authors.lastname: Moon
authors.forename: Sunghee
authors.initials: S
authors.lastname: Cho
authors.forename: Tiing Jen
authors.initials: TJ
authors.lastname: Loh
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhou
authors.forename: Claudia
authors.initials: C
authors.lastname: Ghigna
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Biamonti
authors.forename: Michael R
authors.initials: MR
authors.lastname: Green
authors.forename: Xuexiu
authors.initials: X
authors.lastname: Zheng
authors.forename: Haihong
authors.initials: H
authors.lastname: Shen
mesh_terms: D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation / Q000235:genetics; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006801:Humans; D020033:Protein Isoforms / Q000235:genetics; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D012322:RNA Precursors / Q000235:genetics; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics*; D017384:Sequence Deletion / Q000235:genetics
chemical_list: C000715470:MAS1 protein, human; D020033:Protein Isoforms; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D012322:RNA Precursors; C082332:macrophage stimulating protein; D017228:Hepatocyte Growth Factor; C080835:RON protein; D020794:Receptor Protein-Tyrosine Kinases