title: Assessment of the performance of three multiplex array panels for the detection of circulating cytokines and chemokines in naive, LPS, and SEB-treated cynomolgus macaques.
abstract: To assess relative sensitivity for detection of cytokines and chemokines in cynomolgus serum samples, we tested three commercially available multiplex array kits using the Luminex® platform with sera from animals exposed by intravenous injection to 150 μg/kg staphylococcal enterotoxin B (SEB) or 20 μg/kg lipopolysaccharide (LPS). Each of these kits detected similar patterns of changes in circulating cytokines/chemokines in response to SEB or LPS stimulation, especially the induction of high amounts of interleukin (IL)-2 and interferon-gamma (IFN-γ) in response to SEB but not LPS. However, there were clear differences in sensitivity for particular analytes, especially for IL-10. Additional experiments that focused on one multiplex array kit demonstrated very low or undetectable levels of cytokines in naive cynomolgus macaques, except for highly variable background levels of IL-8, monocyte chemoattractant protein-1, and macrophage inflammatory protein-1β. Therefore, multiplex array analysis of circulating cytokine/chemokine patterns was capable of detection of systemic activation of diverse immune cell subsets.
authors.affiliation: 1Amgen Inc., Seattle, WA, USA.
authors.forename: Ching
authors.initials: C
authors.lastname: He
authors.forename: Padma K
authors.initials: PK
authors.lastname: Narayanan
authors.forename: Madeline M
authors.initials: MM
authors.lastname: Fort
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D018932:Chemokine CCL2 / Q000097:blood*; D054407:Chemokine CCL4 / Q000097:blood; D004768:Enterotoxins / Q000008:administration & dosage / Q000009:adverse effects; D005260:Female; D007371:Interferon-gamma / Q000097:blood*; D016753:Interleukin-10 / Q000097:blood*; D007376:Interleukin-2 / Q000097:blood*; D016209:Interleukin-8 / Q000097:blood*; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000009:adverse effects; D008252:Macaca fascicularis / Q000276:immunology; D008297:Male; D040081:Protein Array Analysis / Q000379:methods*; D011933:Reagent Kits, Diagnostic
chemical_list: D018932:Chemokine CCL2; D054407:Chemokine CCL4; D004768:Enterotoxins; D007376:Interleukin-2; D016209:Interleukin-8; D008070:Lipopolysaccharides; D011933:Reagent Kits, Diagnostic; D016753:Interleukin-10; C031818:enterotoxin B, staphylococcal; D007371:Interferon-gamma
keywords: immune system; inflammation; nonhuman primate.