title: Cryopreservation of interior spruce (Picea glauca engelmanni complex) embryogenic cultures.
abstract: Embryogenic cultures of interior spruce derived from 12 full-sib families were subjected to cryopreservation, with a 97 % success rate for 357 genotypes. Analyses suggested that cryotolerance was not related to family ranking (height increment), embryogenic potential or culture dispersability in suspension, and long-term storage in or above liquid nitrogen did not affect regenerative potential. By contrast, differences in cryotolerance among cell lines appeared to be prevalent in certain families. Analysis with a DNA fingerprinting probe used for clonal identification demonstrated no evidence of somaclonal variation as a result of cryopreservation. The results of this work indicate the applicability of cryopreservation as a long-term storage strategy for spruce embryogenic cultures from a wide genetic background.
authors.affiliation: FBC, BC Research Inc, 3650 Wesbrook Mall, V6S 2L2, Vancouver, B.C., Canada.
authors.forename: D R
authors.initials: DR
authors.lastname: Cyr
authors.forename: W R
authors.initials: WR
authors.lastname: Lazaroff
authors.forename: S M
authors.initials: SM
authors.lastname: Grimes
authors.forename: G
authors.initials: G
authors.lastname: Quan
authors.forename: T D
authors.initials: TD
authors.lastname: Bethune
authors.forename: D I
authors.initials: DI
authors.lastname: Dunstan
authors.forename: D R
authors.initials: DR
authors.lastname: Roberts