title: An autonomously replicating sequence for use in a wide range of budding yeasts.
abstract: The initiation of DNA replication at replication origins is essential for the duplication of genomes. In yeast, the autonomously replicating sequence (ARS) property of replication origins is necessary for the stable maintenance of episomal plasmids. However, because the sequence determinants of ARS function differ among yeast species, current ARS modules are limited for use to a subset of yeasts. Here, we describe a short ARS sequence that functions in at least 10 diverse species of budding yeast. These include, but are not limited to members of the Saccharomyces, Lachancea, Kluyveromyces, and Pichia (Komagataella) genera spanning over 500 million years of evolution. In addition to its wide species range, this ARS and an optimized derivative confer improved plasmid stability relative to other currently used ARS modules.
authors.affiliation: Department of Genome Sciences, University of Washington, Seattle, WA, USA.
authors.forename: Ivan
authors.initials: I
authors.lastname: Liachko
authors.forename: Maitreya J
authors.initials: MJ
authors.lastname: Dunham
mesh_terms: D004261:DNA Replication; D004271:DNA, Fungal; D015870:Gene Expression; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D018741:Replication Origin; D004718:Saccharomycetales / Q000145:classification / Q000235:genetics*
chemical_list: D004271:DNA, Fungal
keywords: Kluyveromyces; Pichia pastoris; Saccharomyces; autonomously replicating sequence; replication origin; yeast