title: Production of Sry knockout mouse using TALEN via oocyte injection.
abstract: Recently developed transcription activator-like effector nuclease (TALEN) technology has enabled the creation of knockout mice, even for genes on the Y chromosome. In this study, we generated a knockout mouse for Sry, a sex-determining gene on the Y chromosome, using microinjection of TALEN RNA into pronuclear stage oocytes. As expected, the knockout mouse had female external and internal genitalia, a female level of blood testosterone and a female sexually dimorphic nucleus in the brain. The knockout mouse exhibited an estrous cycle and performed copulatory behavior as females, although it was infertile or had reduced fertility. A histological analysis showed that the ovary of the knockout mouse displayed a reduced number of oocytes and luteinized unruptured follicles, implying that a reduced number of ovulated oocytes is a possible reason for infertility and/or reduced fertility in the KO mouse.
authors.affiliation: 1] Department of Systems BioMedicine, National Research Institute for Child Health and Development, Tokyo 157-8535, Japan [2].
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kato
authors.forename: Kohei
authors.initials: K
authors.lastname: Miyata
authors.forename: Miku
authors.initials: M
authors.lastname: Sonobe
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yamashita
authors.forename: Moe
authors.initials: M
authors.lastname: Tamano
authors.forename: Kento
authors.initials: K
authors.lastname: Miura
authors.forename: Yoshiakira
authors.initials: Y
authors.lastname: Kanai
authors.forename: Shingo
authors.initials: S
authors.lastname: Miyamoto
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Sakuma
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Masafumi
authors.initials: M
authors.lastname: Inui
authors.forename: Takefumi
authors.initials: T
authors.lastname: Kikusui
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Asahara
authors.forename: Shuji
authors.initials: S
authors.lastname: Takada
mesh_terms: D000818:Animals; D001483:Base Sequence; D004252:DNA Mutational Analysis; D004720:Endonucleases / Q000235:genetics* / Q000378:metabolism*; D030762:Estrous Cycle; D005260:Female; D005298:Fertility; D015870:Gene Expression; D018390:Gene Targeting / Q000379:methods; D006728:Hormones / Q000097:blood; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008845:Microinjections; D008969:Molecular Sequence Data; D009154:Mutation; D009865:Oocytes / Q000378:metabolism*; D010641:Phenotype; D055748:SOXB1 Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D006728:Hormones; D055748:SOXB1 Transcription Factors; D004720:Endonucleases