title: Heterosandwich immunoswab assay for dengue virus Ns1 antigen detection.
abstract: Dengue and the more severe dengue hemorrhagic fever have been a very critical public health problem globally. Millions of people especially in the tropical areas get infected with dengue. An efficient diagnostic is very important for early screening of dengue infection. In dengue-infected patients, the nonstructural protein NS1 is present on the surface of infected cells and secreted in plasma. The NS1 antigen is an important target for developing a quick diagnostic largely due to its long presence in the blood. We have developed a simple-to-use immunoswab-based diagnostic procedure employing monoclonal antibodies and the second-generation quadromas. The detection limit for NS1 has been established to be in the subnanogram range. The assay is very sensitive, has a visual end point, and also being extremely inexpensive. With this assay, screening time for a dengue-infected person would be very rapid.
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 11304-89 Avenue, Edmonton, Alberta Canada, T6G 2 N8.
authors.forename: Advaita
authors.initials: A
authors.lastname: Ganguly
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 11304-89 Avenue, Edmonton, Alberta Canada, T6G 2 N8.
authors.forename: Ravindra B
authors.initials: RB
authors.lastname: Malabadi
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 11304-89 Avenue, Edmonton, Alberta Canada, T6G 2 N8.
authors.forename: Raimer
authors.initials: R
authors.lastname: Loebenberg
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 11304-89 Avenue, Edmonton, Alberta Canada, T6G 2 N8.
authors.forename: Mavanur R
authors.initials: MR
authors.lastname: Suresh
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 11304-89 Avenue, Edmonton, Alberta Canada, T6G 2 N8. Electronic address: hsunwoo@ualberta.ca.
authors.forename: Hoon H
authors.initials: HH
authors.lastname: Sunwoo
mesh_terms: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000097:blood*; D019411:Clinical Laboratory Techniques / Q000191:economics / Q000379:methods*; D003365:Costs and Cost Analysis; D003716:Dengue Virus / Q000302:isolation & purification*; D003955:Diagnostic Tests, Routine / Q000191:economics / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000191:economics / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral
keywords: Bispecific antibody; Dengue; Immunoswab; NS1 protein