title: Screening of protease producing fungi for microbial digestion of seed proteins and synthesis of amino acids-metalnutrient chelates.
abstract: The problem of metalnutrient deficiency is becoming more serious with the introduction of modern agricultural practices. As a result, metalnutrient deficiency is recognized as one of the critical yield limiting factors. Metalnutrients are generally offered in their sulphate or oxide forms. However, it is reported that organically bound minerals generally have a higher bioavailability than inorganic minerals. Chelation makes otherwise unavailable metalnutrients plant available. Amino acids are well known among various chelating agents. In present investigation the fungus Paecilomyces variotii PR-4 was isolated from soil and was used for production of protease and determination of its activity. Proteins from germinating seeds of chick pea, mung bean, soybean and cowpea were hydrolyzed for the production of amino acids. Amino acids were recovered, estimated and utilized for chelation of metalnutrients viz., Zn, Cu, Fe, Mn, Mg, B and Mo. The resultant chelates were employed to detect with Fourier Transform Infra-Red Spectrophotometer (FTIR) analysis. The peaks of most intensive bands in the IR spectra of ligands recorded were present in the intervals of the wave numbers 3500-3300 and 1720-1700 cm(-1). Chelation of metalnutrients led to the broadening of peak and changes of the peak position of hydroxyl groups, which indicated the binding of the carboxylic groups and primary amine groups of amino acids to the metalnutrients. The resultant amino acids-metalnutrient chelates can be utilized as organic fertilizer.
authors.affiliation: Department of Botany, Fergusson College, Pune (M.S.) 411 004, India.
authors.forename: G B
authors.initials: GB
authors.lastname: Deore
authors.forename: A S
authors.initials: AS
authors.lastname: Limaye
authors.forename: Y A
authors.initials: YA
authors.lastname: Dushing
authors.forename: S B
authors.initials: SB
authors.lastname: Dhobale
authors.forename: S
authors.initials: S
authors.lastname: Kale
authors.forename: S L
authors.initials: SL
authors.lastname: Laware
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D002614:Chelating Agents / Q000378:metabolism*; D007887:Fabaceae / Q000196:embryology / Q000378:metabolism*; D010143:Paecilomyces / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000096:biosynthesis*; D010940:Plant Proteins / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000596:Amino Acids; D002614:Chelating Agents; D010940:Plant Proteins; D010447:Peptide Hydrolases