title: Molecular responses of calreticulin gene to Vibrio anguillarum and WSSV challenge in the ridgetail white prawn Exopalaemon carinicauda.
abstract: Calreticulin (CRT), as a highly conserved endoplasmic reticulum luminal resident protein, plays important roles in Ca(2+) homeostasis, molecular chaperoning and response to viral infection. In this study, a full-length cDNA of CRT (designated EcCRT) was cloned from hemocytes of the ridgetail white prawn Exopalaemon carinicauda by using rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of EcCRT was 1725 bp, which contains a 5'-untranslated region (UTR) of 57 bp, 3'-UTR of 453 bp with a poly (A) tail, an open reading frame (ORF) of 1215 bp, encoding a 404 amino-acid polypeptide with the predicted molecular weight of 46.51 kDa and estimated isoelectric point of 4.32. The deduced amino acid sequence of EcCRT shared high identity (82%-85%) with that of other crustaceans. Phylogenetic analysis showed that EcCRT of E. carinicauda was clustered together with CRT of other shrimps, indicating that EcCRT should be a member of the CRT family. Quantitative real-time RT-qPCR analysis indicated that EcCRT was expressed in hemocytes, gill, hepatopancreas, muscle, ovary, intestine, stomach and eyestalk, with the highest expression level in hemocytes. After Vibrio anguillarum and WSSV challenge, the expression level of EcCRT transcripts both in the hemocytes and hepatopancreas of E. carinicauda were up-regulated in the first 6 h, respectively. The results suggested that EcCRT might be associated with the immune defenses to V. anguillarum and WSSV in E. carinicauda.
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, PR China; Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China.
authors.forename: Yafei
authors.initials: Y
authors.lastname: Duan
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, PR China. Electronic address: liuping@ysfri.ac.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, PR China.
authors.forename: Jitao
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D037282:Calreticulin / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D033541:Palaemonidae / Q000235:genetics / Q000276:immunology* / Q000821:virology; D010802:Phylogeny; D012313:RNA / Q000737:chemistry / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014733:Vibrio / Q000276:immunology*; D014735:Vibrio Infections / Q000276:immunology*; D046848:White spot syndrome virus 1 / Q000276:immunology*
chemical_list: D037282:Calreticulin; D012313:RNA
keywords: Calreticulin (CRT); Exopalaemon carinicauda; Gene cloning; Vibrio anguillarum; White spot syndrome virus (WSSV)