title: Access channel residues Ser315 and Asp137 in Mycobacterium tuberculosis catalase-peroxidase (KatG) control peroxidatic activation of the pro-drug isoniazid.
abstract: Peroxidatic activation of the anti-tuberculosis pro-drug isoniazid by Mycobacterium tuberculosis catalase-peroxidase (KatG) is regulated by gating residues of a heme access channel. The steric restriction at the bottleneck of this channel is alleviated by replacement of residue Asp137 with Ser, according to crystallographic and kinetic studies.
authors.affiliation: Department of Chemistry, Brooklyn College and The Graduate Center of The City University of New York, 2900 Bedford Avenue, Brooklyn, NY 11210, USA. rmaglioz@brooklyn.cuny.edu.
authors.forename: Xiangbo
authors.initials: X
authors.lastname: Zhao
authors.forename: Hans-Petter
authors.initials: HP
authors.lastname: Hersleth
authors.forename: Janan
authors.initials: J
authors.lastname: Zhu
authors.forename: K Kristoffer
authors.initials: KK
authors.lastname: Andersson
authors.forename: Richard S
authors.initials: RS
authors.lastname: Magliozzo
mesh_terms: D000995:Antitubercular Agents / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D002374:Catalase / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007538:Isoniazid / Q000378:metabolism*; D008958:Models, Molecular; D009169:Mycobacterium tuberculosis / Q000201:enzymology*; D011355:Prodrugs / Q000378:metabolism*; D014376:Tuberculosis / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents; D001426:Bacterial Proteins; D011355:Prodrugs; D002374:Catalase; C480353:katG protein, Mycobacterium tuberculosis; D007538:Isoniazid