title: Phytase production by Rhizopus microsporus var. microsporus biofilm: characterization of enzymatic activity after spray drying in presence of carbohydrates and nonconventional adjuvants.
abstract: Microbial phytases are enzymes with biotechnological interest for the feed industry. In this article, the effect of spray-drying conditions on the stability and activity of extracellular phytase produced by R. microsporus var. microsporus biofilm is described. The phytase was spray-dried in the presence of starch, corn meal (>150 μm), soy bean meal (SB), corn meal (<150 μm) (CM), and maltodextrin as drying adjuvants. The residual enzyme activity after drying ranged from 10.7% to 60.4%, with SB and CM standing out as stabilizing agents. Water concentration and residual enzyme activity were determined in obtained powders as a function of the drying condition. When exposed to different pH values, the SB and CM products were stable, with residual activity above 50% in the pH range from 4.5 to 8.5 for 60 min. The use of CM as drying adjuvant promoted the best retention of enzymatic activity compared with SB. Spray drying of the R. microsporus var. microsporus phytase using different drying adjuvants showed interesting results, being quite feasible with regards their biotechnological applications, especially for poultry diets.
authors.affiliation: Institute of Chemistry of Araraquara - UNESP, Rua Francisco Degni s/n, 14800-900 Araraquara, São Paulo, Brazil.
authors.forename: Vanessa Sayuri
authors.initials: VS
authors.lastname: Sato
authors.forename: João Atílio
authors.initials: JA
authors.lastname: Jorge
authors.forename: Wanderley Pereira
authors.initials: WP
authors.lastname: Oliveira
authors.forename: Claudia Regina Fernandez
authors.initials: CR
authors.lastname: Souza
authors.forename: Luis Henrique Souza
authors.initials: LH
authors.lastname: Guimarães
mesh_terms: D010832:6-Phytase / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D050260:Carbohydrate Metabolism; D003890:Desiccation; D006863:Hydrogen-Ion Concentration; D012233:Rhizopus / Q000201:enzymology* / Q000502:physiology*; D013997:Time Factors; D003313:Zea mays / Q000378:metabolism*
chemical_list: D010832:6-Phytase