title: Targeted exome capture and sequencing identifies novel PRPF31 mutations in autosomal dominant retinitis pigmentosa in Chinese families.
abstract: OBJECTIVE
  To identify disease-causing mutations in two Chinese families with autosomal dominant retinitis pigmentosa (adRP).
  
  
  METHODS
  Prospective analysis.
  
  
  METHODS
  Two Chinese adRP families underwent genetic diagnosis. A specific hereditary eye disease enrichment panel (HEDEP) based on targeted exome capture technology was used to collect the protein coding regions of targeted 371 hereditary eye disease genes; high throughput sequencing was done with the Illumina HiSeq 2000 platform. The identified variants were confirmed with Sanger sequencing.
  
  
  METHODS
  All experiments were performed in a large laboratory specialising in genetic studies in the Department of Ophthalmology, Peking University Third Hospital.
  
  
  RESULTS
  Two novel mutations, including one splice site mutation (Int10 c.1074-2 A>T; p.Y359SfsX29) and one insertion (c.824_825insA; p.Y275X) of PRPF31 were identified in the two families. The two mutations segregated with the disease phenotype in their respective families.
  
  
  CONCLUSIONS
  Our findings broaden the spectrum of PRPF31 mutations causing adRP and the phenotypic spectrum of the disease in Chinese patients. The HEDEP based on targeted exome capture technology is an efficient method for molecular diagnosis in adRP patients.
authors.affiliation: Department of Ophthalmology, Peking University Third Hospital, Key Laboratory of Vision Loss and Restoration, Ministry of Education, Beijing, P. R. China.
authors.forename: Liping
authors.initials: L
authors.lastname: Yang
authors.forename: Xiaobei
authors.initials: X
authors.lastname: Yin
authors.forename: Lemeng
authors.initials: L
authors.lastname: Wu
authors.forename: Ningning
authors.initials: N
authors.lastname: Chen
authors.forename: Huirong
authors.initials: H
authors.lastname: Zhang
authors.forename: Genlin
authors.initials: G
authors.lastname: Li
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Ma