title: Rice black-streaked dwarf virus P10 induces membranous structures at the ER and elicits the unfolded protein response in Nicotiana benthamiana.
abstract: Endoplasmic reticular (ER) membrane modifications play an important role in viral RNA replication and virion assembly but little is known about the involvement of ER-membrane remodeling in the infection cycle of fijiviruses in plant cells. The subcellular localization of Rice black-streaked dwarf virus outer capsid P10 was therefore examined using live-cell imaging. P10 fused to eGFP formed vesicular structures associated with ER membranes in Nicotiana benthamiana epidermal cells and in rice protoplasts. Subcellular fractionation experiments confirmed that P10 is an integral membrane protein. Three predicted transmembrane domains and two less-well-defined domains were each able to target eGFP to the ER. Disruption of the actin cytoskeleton with LatB, indicated that the maintenance of P10-induced membrane structures required the intact actin cytoskeleton. P10 induced the expression of ER stress marker genes, including ER stress-related chaperones and transcription factor, indicating that RBSDV P10 triggers ER stress and the unfolded protein response.
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Zhejiang Provincial key laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
authors.forename: Zongtao
authors.initials: Z
authors.lastname: Sun
authors.forename: Di
authors.initials: D
authors.lastname: Yang
authors.forename: Li
authors.initials: L
authors.lastname: Xie
authors.forename: Liying
authors.initials: L
authors.lastname: Sun
authors.forename: Shanglin
authors.initials: S
authors.lastname: Zhang
authors.forename: Qisong
authors.initials: Q
authors.lastname: Zhu
authors.forename: Junmin
authors.initials: J
authors.lastname: Li
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Jianping
authors.initials: J
authors.lastname: Chen
mesh_terms: D019677:Artificial Gene Fusion; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism / Q000821:virology*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D054884:Host-Pathogen Interactions; D012275:Oryza / Q000821:virology; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics; D012087:Reoviridae / Q000502:physiology*; D014026:Nicotiana / Q000821:virology; D056811:Unfolded Protein Response
chemical_list: D036022:Capsid Proteins; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
keywords: Capsid protein P10; ER membrane modification; ER stress; Rice black-streaked dwarf virus; Unfolded protein response