title: Down-regulation of miR-129-5p inhibits growth and induces apoptosis in laryngeal squamous cell carcinoma by targeting APC.
abstract: miRNAs regulate gene expression and are key mediators of tumourigenesis. miR-129 has diverse effects in tumours, but its role in laryngeal squamous cell carcinoma (LSCC) remains unknown. This article focuses on the role of miR-129-5p in LSCC. We show miR-129-5p is upregulated in primary LSCC tumours and correlated with advanced disease. Down-regulating miR-129-5p suppressed cell proliferation and migration, and caused cell cycle arrest in Hep-2 cell lines. Downregulation of miR-129-5p alone is sufficient to induce apoptosis both in vivo and in vitro. Moreover, the growth of LSCC xenograft exposed to miR-129-5p antisense oligonucleotides (ASO) in BALB/c mice was markedly inhibited. In addition, we found that miR-129-5p targeted adenomatous polyposis coli (APC) to release inhibition of Wnt signalling causing cell growth and tumourigenesis. Our results suggest miR-129-5p functions as an oncogene in LSCC by repressing APC and is a potential therapeutic target for LSCC.
authors.affiliation: Services of Head and Neck Surgery, department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: MingHua
authors.initials: M
authors.lastname: Li
authors.forename: LinLi
authors.initials: L
authors.lastname: Tian
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: HongChao
authors.initials: H
authors.lastname: Yao
authors.forename: JiaRui
authors.initials: J
authors.lastname: Zhang
authors.forename: JianGuang
authors.initials: J
authors.lastname: Lu
authors.forename: YaNan
authors.initials: Y
authors.lastname: Sun
authors.forename: Xu
authors.initials: X
authors.lastname: Gao
authors.forename: Hui
authors.initials: H
authors.lastname: Xiao
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
mesh_terms: D025601:Adenomatous Polyposis Coli Protein / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000235:genetics
chemical_list: D025601:Adenomatous Polyposis Coli Protein; D035683:MicroRNAs; C541915:Mirn129 microRNA, human; D016376:Oligonucleotides, Antisense