title: Characterization of three Chlorella sorokiniana strains in anaerobic digested effluent from cattle manure.
abstract: Chlorella sorokiniana CS-01, UTEX 1230 and UTEX 2714 were maintained in 10% anaerobic digester effluent (ADE) from cattle manure digestion and compared with algal cultivation in Bold's Basal Medium (BBM). Biomass of CS-01 and UTEX 1230 in ADE produced similar or greater than 280mg/L after 21days in BBM, however, UTEX 2714 growth in ADE was suppressed by more than 50% demonstrating a significant species bias to synthetic compared to organic waste-based media. The highest accumulation of protein and starch was exhibited in UTEX 1230 in ADE yielding 34% and 23% ash free dry weight (AFDW), respectively, though fatty acid methyl ester total lipid measured less than 12% AFDW. Results suggest that biomass from UTEX 1230 in ADE may serve as a candidate alga and growth system combination sustainable for animal feed production considering high yields of protein, starch and low lipid accumulation.
authors.affiliation: Department of Biochemistry, University of Nebraska-Lincoln, 1901 Vine Street, Lincoln, NE 68588, United States.
authors.forename: Naoko
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Eric A
authors.initials: EA
authors.lastname: Noel
authors.forename: Austin
authors.initials: A
authors.lastname: Barnes
authors.forename: Andrea
authors.initials: A
authors.lastname: Watson
authors.forename: Julian N
authors.initials: JN
authors.lastname: Rosenberg
authors.forename: Galen
authors.initials: G
authors.lastname: Erickson
authors.forename: George A
authors.initials: GA
authors.lastname: Oyler
mesh_terms: D064751:Ammonium Compounds / Q000378:metabolism; D000693:Anaerobiosis; D000818:Animals; D018533:Biomass; D002417:Cattle; D002708:Chlorella / Q000187:drug effects / Q000254:growth & development*; D003470:Culture Media / Q000494:pharmacology; D004952:Esters / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D008372:Manure; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D013213:Starch / Q000378:metabolism; D014865:Waste Disposal, Fluid
chemical_list: D064751:Ammonium Compounds; D003470:Culture Media; D004952:Esters; D005227:Fatty Acids; D008372:Manure; D009566:Nitrates; D009573:Nitrites; D010758:Phosphorus; D013213:Starch; D009584:Nitrogen
keywords: Anaerobic digester effluent; Biomass; Chlorella sorokiniana; Nutrient removal; Starch