title: Role of mitochondria-cytoskeleton interactions in respiration regulation and mitochondrial organization in striated muscles.
abstract: The aim of this work was to study the regulation of respiration and energy fluxes in permeabilized oxidative and glycolytic skeletal muscle fibers, focusing also on the role of cytoskeletal protein tubulin βII isotype in mitochondrial metabolism and organization. By analyzing accessibility of mitochondrial ADP, using respirometry and pyruvate kinase-phosphoenolpyruvate trapping system for ADP, we show that the apparent affinity of respiration for ADP can be directly linked to the permeability of the mitochondrial outer membrane (MOM). Previous studies have shown that MOM permeability in cardiomyocytes can be regulated by VDAC interaction with cytoskeletal protein, βII tubulin. We found that in oxidative soleus skeletal muscle the high apparent Km for ADP is associated with low MOM permeability and high expression of non-polymerized βII tubulin. Very low expression of non-polymerized form of βII tubulin in glycolytic muscles is associated with high MOM permeability for adenine nucleotides (low apparent Km for ADP).
authors.affiliation: INSERM U1055, Laboratory of Fundamental and Applied Bioenergetics, Joseph Fourier University, Grenoble, France; Tallinn University of Technology, Faculty of Science, Centre for Biology of Integrated Systems, Estonia.
authors.forename: Minna
authors.initials: M
authors.lastname: Varikmaa
authors.affiliation: INSERM U1055, Laboratory of Fundamental and Applied Bioenergetics, Joseph Fourier University, Grenoble, France; Experimental, Theoretical and Applied Cardio-Respiratory Physiology, Laboratory TIMC-IMAG, Joseph Fourier University, Grenoble, France.
authors.forename: Rafaela
authors.initials: R
authors.lastname: Bagur
authors.affiliation: Laboratory of Bioenergetics, National Institute of Chemical Physics and Biophysics, Tallinn, Estonia.
authors.forename: Tuuli
authors.initials: T
authors.lastname: Kaambre
authors.affiliation: Life Science Imaging - In Vitro Platform, IAB CRI U823 Inserm, Joseph Fourier University, Grenoble, France.
authors.forename: Alexei
authors.initials: A
authors.lastname: Grichine
authors.affiliation: Laboratory of Bioenergetics, National Institute of Chemical Physics and Biophysics, Tallinn, Estonia.
authors.forename: Natalja
authors.initials: N
authors.lastname: Timohhina
authors.affiliation: Laboratory of Bioenergetics, National Institute of Chemical Physics and Biophysics, Tallinn, Estonia.
authors.forename: Kersti
authors.initials: K
authors.lastname: Tepp
authors.affiliation: Laboratory of Bioenergetics, National Institute of Chemical Physics and Biophysics, Tallinn, Estonia.
authors.forename: Igor
authors.initials: I
authors.lastname: Shevchuk
authors.affiliation: Laboratory of Bioenergetics, National Institute of Chemical Physics and Biophysics, Tallinn, Estonia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Chekulayev
authors.affiliation: Tallinn University of Technology, Faculty of Science, Centre for Biology of Integrated Systems, Estonia.
authors.forename: Madis
authors.initials: M
authors.lastname: Metsis
authors.affiliation: Experimental, Theoretical and Applied Cardio-Respiratory Physiology, Laboratory TIMC-IMAG, Joseph Fourier University, Grenoble, France.
authors.forename: François
authors.initials: F
authors.lastname: Boucher
authors.affiliation: INSERM U1055, Laboratory of Fundamental and Applied Bioenergetics, Joseph Fourier University, Grenoble, France; Laboratory of Bioenergetics, National Institute of Chemical Physics and Biophysics, Tallinn, Estonia.
authors.forename: Valdur
authors.initials: V
authors.lastname: Saks
authors.affiliation: Cardiac Surgery Laboratory, Department of Heart Surgery, Innsbruck Medical University, Innsbruck A-6020, Austria.
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Kuznetsov
authors.affiliation: INSERM U1055, Laboratory of Fundamental and Applied Bioenergetics, Joseph Fourier University, Grenoble, France; Department of Rehabilitation and Physiology, University Hospital Grenoble, France. Electronic address: rita.guzun@gmail.com.
authors.forename: Rita
authors.initials: R
authors.lastname: Guzun
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D019069:Cell Respiration; D003598:Cytoskeletal Proteins / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism*; D004734:Energy Metabolism; D008297:Male; D064688:Metabolic Flux Analysis; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000378:metabolism*; D051336:Mitochondrial Membranes / Q000378:metabolism; D054792:Muscle, Striated / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D010539:Permeability; D051381:Rats; D017208:Rats, Wistar; D014404:Tubulin / Q000378:metabolism
chemical_list: D003598:Cytoskeletal Proteins; D014404:Tubulin; D000244:Adenosine Diphosphate
keywords: Energy flux; Intracellular energy unit; Metabolic control analysis; Respiration; Skeletal muscle