title: Clonal multiplication of Gardenia jasminoides Ellis through axillary bud culture.
abstract: An efficient clonal multiplication system was developed for in vitro propagation of crocin - producing Gardenia jasminoides Ellis plants. Murashige and Skoog's (MS) medium containing 6-benzylaminopurine (BAP 1 mg l(-1)) and indole-3-butyric acid (IBA 1 mg l(-1)) resulted in multiple shoot initiation at the rate of 21 shoots per explant in 60 d of culture. Transfer of the microshoots into liquid MS medium supplemented with BAP (5 mg l(-1)) with two subcultures of 15 d duration in the same medium resulted in 400 ± 25 shoots per explant. Efficient rooting was achieved in MS medium supplemented with α-naphthaleneacetic acid (5 mg l(-1)). The in vitro raised plants were hardened in a greenhouse and transplanted to the field successfully. The method described will be useful for rapid multiplication of Gardenia for commercial exploitation.
authors.affiliation: Department of Plant Cell Biotechnology, Central Food Technological Research Institute, 570 013, Mysore, India.
authors.forename: P S
authors.initials: PS
authors.lastname: George
authors.forename: G A
authors.initials: GA
authors.lastname: Ravishankar
authors.forename: L V
authors.initials: LV
authors.lastname: Venkataraman