title: An RFLP linkage map for loblolly pine based on a three-generation outbred pedigree.
abstract: A genetic linkage map for loblolly pine (Pinus taeda L.) was constructed using segregation data from a three-generation outbred pedigree consisting of four grandparents, two parents, and 95 F2 progeny. The map was based predominantly on restriction fragment length polymorphism (RFLP) loci detected by cDNA probes. Sixty-five cDNA and three genomic DNA probes revealed 90 RFLP loci. Six polymorphic isozyme loci were also scored. One-fourth (24%) of the cDNA probes detected more than 1 segregating locus, an indication that multigene families are common in pines. As many as six alleles were observed at a single segregating locus among grandparents and it was not unusual for the progeny to segregate for three or four alleles per locus. Multipoint linkage analysis placed 73 RFLP and 2 isozyme loci into 20 linkage groups; the remaining 17 RFLP and 4 isozyme loci were unlinked. The mapped RFLP probes provide a new set of codominant markers for genetic analyses in loblolly pine.
authors.affiliation: Division of Forestry/CSIRO, Queen Victoria Terrace, P.O. Box 4008, 2600, Canberra, ACT, Australia.
authors.forename: M E
authors.initials: ME
authors.lastname: Devey
authors.forename: T A
authors.initials: TA
authors.lastname: Fiddler
authors.forename: B H
authors.initials: BH
authors.lastname: Liu
authors.forename: S J
authors.initials: SJ
authors.lastname: Knapp
authors.forename: D B
authors.initials: DB
authors.lastname: Neale