title: Agrobacterium induced gall formation in lipoxygenase mutant isolines of soybeans.
abstract: Agrobacterium-mediated transformation frequency is very low with cells from some species such as soybeans. Studies were conducted to investigate the Agrobacterium-mediated transformation frequency in near-isogenic lipoxygenase mutant lines of soybeans, since the nigh level of lipoxygenase activity in soybean embryos might be expected to affect interactions with Agrobacterium. The mutant line lacking lipoxygenase 3 showed significantly greater frequency of Agrobacterium-induced transformation than the other soybean lines. Stages of soybean embryo development which showed maximum differences in lipoxygenase 3 activity between mutant and wild-type, also showed maximum differences in transformation frequency. The increased transformation frequency with the absence of lipoxygenase 3 was only seen when both lipoxygenase 1 and 2 were present.
authors.affiliation: Unicorn Biotek Ltd., 1-10-44/1/1, Chikoti Gardens, Begumpet, 500016, Hyderabad, India.
authors.forename: D
authors.initials: D
authors.lastname: Bhatt
authors.forename: W A
authors.initials: WA
authors.lastname: Parrott
authors.forename: G B
authors.initials: GB
authors.lastname: Collins
authors.forename: D F
authors.initials: DF
authors.lastname: Hildebrand