title: Hypercholesterolemia and effects of high cholesterol diet in type IIa sodium-dependent phosphate co-transporter (Npt2a) deficient mice.
abstract: The type IIa sodium-dependent phosphate co-transporter (Npt2a) is important to maintain renal inorganic phosphate (Pi) homeostasis and the plasma Pi levels. It has reported that disorder of Pi metabolism in kidney can be risk factors for cardiovascular disease as well as hypercholesterolemia. However, the relationship between Pi and cholesterol metabolism has not been clarified. The current study investigated the effects of Npt2a gene ablation that is known as hypophosphatemia model on cholesterol metabolism in mice. Npt2a deficient (Npt2a(-/-)) mice and wild type mice were fed diets with or without 2% cholesterol for 12 days. Plasma lipid and lipoprotein profile analysis revealed that plasma lipid levels (total, LDL and HDL cholesterol) were significantly higher in Npt2a(-/-) mice than wild type (WT) mice. Interestingly, high cholesterol diet markedly increased plasma levels of total, LDL and HDL cholesterol in WT mice, but not Npt2a(-/-) mice. On the other hand, there were no differences in body and liver weight, intake and hepatic lipid accumulation between WT and Npt2a(-/-) mice. These results suggest that ablation of Npt2a gene induces hypercholesterolemia and affects the ability to respond normally to dietary cholesterol.
authors.affiliation: Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School.
authors.forename: Sarasa
authors.initials: S
authors.lastname: Tanaka
authors.forename: Hironori
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Otoki
authors.initials: O
authors.lastname: Nakahashi
authors.forename: Mariko
authors.initials: M
authors.lastname: Ishiguro
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Takei
authors.forename: Masashi
authors.initials: M
authors.lastname: Masuda
authors.forename: Mina
authors.initials: M
authors.lastname: Kozai
authors.forename: Shoko
authors.initials: S
authors.lastname: Ikeda
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Taketani
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Eiji
authors.initials: E
authors.lastname: Takeda
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D005260:Female; D006937:Hypercholesterolemia / Q000097:blood / Q000209:etiology / Q000378:metabolism*; D008055:Lipids / Q000097:blood; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010710:Phosphates / Q000097:blood; D050613:Sodium-Phosphate Cotransporter Proteins, Type IIa / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism
chemical_list: D002791:Cholesterol, Dietary; D008055:Lipids; D010710:Phosphates; C493555:Slc34a1 protein, mouse; D050613:Sodium-Phosphate Cotransporter Proteins, Type IIa; D002784:Cholesterol