title: [Diversity and in vitro antitumnor activity of endophytic fungi from mangrove plants Xylocarpus].
abstract: A total of 24 biologically pure entophytic fungal strains were isolated from stems, leaves, and seed coats of Xylocarpus plants by repeated purification, and identified with Internal Transcribed Spacer (ITS) rDNA molecular method, which belonging to 14 genera, 11 families, 9 orders and 3 classes. There were differences in genus and species levels among three plant materials from different habitats and species, and it was found that the strains of Phomopsis and Colletotrichum existed in all three plant materials. In vitro assay of antitumor activity by MTT method revealed that the EtOAc extracts of 15 strains exhibited potent antitumor activity. These results suggest that it is of value for further investigation on the above fungal strains.
authors.affiliation: College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100102, China. ningjianger@163.com
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Fei-Ying
authors.initials: FY
authors.lastname: Ruan
authors.forename: Zheng-Shun
authors.initials: ZS
authors.lastname: Wen
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Li
authors.forename: Ri-Dao
authors.initials: RD
authors.lastname: Chen
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.forename: Dan
authors.initials: D
authors.lastname: Xie
authors.forename: Min-Yi
authors.initials: MY
authors.lastname: Li
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Jun-Gui
authors.initials: JG
authors.lastname: Dai
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D044822:Biodiversity; D045744:Cell Line, Tumor; D060026:Endophytes / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D005658:Fungi / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D045325:HCT116 Cells; D006801:Humans; D029582:Meliaceae / Q000382:microbiology*; D010802:Phylogeny
chemical_list: D000970:Antineoplastic Agents