title: Sequence analysis of bovine C/EBPδ gene and its adipogenic effects on fibroblasts.
abstract: CCAAT/enhancer binding protein delta (C/EBPδ), an important transcriptional factor, regulates cell growth, differentiation and adipogenesis in humans and mice. However, we lack of directive information on the effects of C/EBPδ gene in bovine cells. In the present study, we cloned the CDS areas of bovine C/EBPδ gene and predicted its sequence characteristics. Moreover, we constructed the recombinant adenovirus plasmids of bovine C/EBPδ gene and harvested the subsequent adenoviruses to infect bovine primary fibroblasts. Oil Red O staining results showed lipid droplets accumulated gradually in the adenoviruses treated fibroblasts. Time course real-time PCR results indicated that over-expression of exogenous C/EBPδ regulated the mRNA expression levels of some key adipogenic genes, herein, activated the C/EBPα expression, increased lipoprotein lipase and fatty acid binding protein 4 mRNA expression levels, whereas inhibited leptin receptor gene. In conclusion, the present study demonstrates that the elevated C/EBPδ can induce the adipogenesis in the fibroblasts of cattle.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Gong
authors.initials: G
authors.lastname: Cheng
authors.forename: Changzhen
authors.initials: C
authors.lastname: Fu
authors.forename: Hongbao
authors.initials: H
authors.lastname: Wang
authors.forename: Wucai
authors.initials: W
authors.lastname: Yang
authors.forename: Hongcheng
authors.initials: H
authors.lastname: Wang
authors.forename: Linsen
authors.initials: L
authors.lastname: Zan
mesh_terms: D016475:3T3 Cells; D050156:Adipogenesis; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics / Q000378:metabolism; D051743:CCAAT-Enhancer-Binding Protein-delta / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D005347:Fibroblasts / Q000502:physiology*; D015870:Gene Expression; D005786:Gene Expression Regulation; D050356:Lipid Metabolism; D051379:Mice; D008969:Molecular Sequence Data; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D047495:PPAR gamma; D051743:CCAAT-Enhancer-Binding Protein-delta