title: Germination of encapsulated embryos of interior spruce (Picea glauca engelmannii complex) and black spruce (Picea mariana Mill.).
abstract: Interior spruce (Picea glauca engelmannii complex) and black spruce (Picea mariana Mill.) cotyledonary somatic embryos were encapsulated in sodium alginate. Somatic embryo viability was retained, but germination occurred at a reduced frequency compared with the equivalent zygotic embryos. The addition of 0.5% (w/v) activated charcoal to the alginate capsule significantly enhanced root development and germination for somatic embryos but not for zygotic embryos. The possibility of developing an artiflcal endosperm was also investigated, by addition of Litvay (Litvay et al. 1981) nutrients with or without 90 mM sucrose to the alginate-charcoal capsule. This treatment significantly enhanced root development for all embryo categories with the exception of black spruce somatic embryos. Encapsulated and non-encapsulated somatic embryos survived one month cold storage at 4 °C without reduction in germination frequency.
authors.affiliation: Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, S7N 0W9, Saskatoon, Saskatchewan, Canada.
authors.forename: M M
authors.initials: MM
authors.lastname: Lulsdorf
authors.forename: T E
authors.initials: TE
authors.lastname: Tautorus
authors.forename: S I
authors.initials: SI
authors.lastname: Kikcio
authors.forename: T D
authors.initials: TD
authors.lastname: Bethune
authors.forename: D I
authors.initials: DI
authors.lastname: Dunstan