title: Partitioning and remodeling of the Schizosaccharomyces japonicus mitotic nucleus require chromosome tethers.
abstract: During cellular proliferation, the mother nucleus divides into two daughters, each carrying a full complement of chromosomes and capable of driving the next cell cycle. Mitotic chromosome segregation must be coordinated with remodeling of other nuclear components, including the nuclear envelope (NE) and the non-membrane-bound nucleolus. Here, we show that in the fission yeast Schizosaccharomyces japonicus, which breaks and reforms the NE during mitosis, the evolutionary conserved LEM-domain protein Man1 promotes equal partitioning of the nuclear membrane, nucleolar remodeling, and efficient inheritance of the nuclear pore complexes (NPCs) by the daughter nuclei. Analyses of man1 mutants and results obtained using an artificial chromatin-nuclear pore tether suggest that Man1 may exert its function by linking the NPCs to segregating chromatin. By integrating the partitioning of the nuclear membrane and nucleolar material with chromosome segregation, cells build two nearly identical copies of the original nucleus.
authors.affiliation: Temasek Life Sciences Laboratory, 1 Research Link, Singapore 117604, Singapore; Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore.
authors.forename: Candice
authors.initials: C
authors.lastname: Yam
authors.affiliation: Temasek Life Sciences Laboratory, 1 Research Link, Singapore 117604, Singapore.
authors.forename: Ying
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Temasek Life Sciences Laboratory, 1 Research Link, Singapore 117604, Singapore; Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore. Electronic address: snezhana.oliferenko@kcl.ac.uk.
authors.forename: Snezhana
authors.initials: S
authors.lastname: Oliferenko
mesh_terms: D000705:Anaphase / Q000235:genetics; D002466:Cell Nucleolus / Q000235:genetics; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D008938:Mitosis; D009154:Mutation; D009685:Nuclear Envelope / Q000235:genetics; D022022:Nuclear Pore / Q000235:genetics* / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics*; D008941:Spindle Apparatus
chemical_list: D002843:Chromatin; D005656:Fungal Proteins; D009687:Nuclear Proteins