title: Imaging of RNAs in live cells with spectrally diverse small molecule fluorophores.
abstract: We have developed new protein-based tags for use in imaging of RNAs in living cells. These tags are based on the MS2 phage coat protein fused to either E. coli dihydrofolate reductase or the SNAP tag. Labeled RNAs can be visualized with fluorophores spanning the visible spectra range.
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Dr., Madison, WI 53706, USA. ahoskins@wisc.edu.
authors.forename: Tucker J
authors.initials: TJ
authors.lastname: Carrocci
authors.forename: Aaron A
authors.initials: AA
authors.lastname: Hoskins
mesh_terms: D004926:Escherichia coli / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D017909:Levivirus / Q000737:chemistry; D008859:Microscopy, Polarization / Q000379:methods; D061848:Optical Imaging / Q000379:methods*; D012313:RNA / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000166:cytology*
chemical_list: D005456:Fluorescent Dyes; D012313:RNA