title: High efficiency plant regeneration from petiole explants of Carica papaya L. through organogenesis.
abstract: Callus cultures were obrained from petiole explants of Carica papaya on MS medium containing 0.5-10.5 μM α-naphthaleneacetic acid (NAA) in combination with 0.5-5 μM benzyladenine (BA). Hard-green calli were transferred to MS medium containing 100 mgl(-1) casein hydrolysate (CH) with specific BA-NAA formulation, where they developed adventitious buds within 2 weeks of culture. Maximum number of adventitious buds were obtained in 2 μM BA and 0.1 μM NAA. Shoot regeneration occurred from these adventitious buds by the end of the 4th week. Regenerated shoots were elongated in hormone-free medium and rooted in half-strength MS fortified with 3 UM NAA and 0.5 μM gibberellic acid (GA3). The regenerants were transferred to soil after acclimatization.
authors.affiliation: Department of Botany, Rajshahi University, 6205, Rajshahi, Bangladesh.
authors.forename: M
authors.initials: M
authors.lastname: Hossain
authors.forename: S M
authors.initials: SM
authors.lastname: Rahman
authors.forename: R
authors.initials: R
authors.lastname: Islam
authors.forename: O I
authors.initials: OI
authors.lastname: Joarder