title: A baculoviral display system to assay viral entry.
abstract: In this study, we evaluated a baculoviral display system for analysis of viral entry by using a recombinant adenovirus (Ad) carrying a luciferase gene and budded baculovirus (BV) that displays the adenoviral receptor, coxsackievirus and adenovirus receptor (CAR). CAR-expressing B16 cells (B16-CAR cells) were infected with luciferase-expressing Ad vector in the presence of BV that expressed or lacked CAR (CAR-BV and mock-BV, respectively). Treatment with mock-BV even at doses as high as 5 µg/mL failed to attenuate the luciferase activity of B16-CAR cells. In contrast, treatment with CAR-BV with doses as low as 0.5 µg/mL significantly decreased the luciferase activity of infected cells, which reached 65% reduction at 5 µg/mL. These findings suggest that a receptor-displaying BV system could be used to evaluate viral infection.
authors.affiliation: Laboratory of Bio-Functional Molecular Chemistry, Graduate School of Pharmaceutical Sciences, Osaka University.
authors.forename: Manami
authors.initials: M
authors.lastname: Iida
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yoshida
authors.forename: Akihiro
authors.initials: A
authors.lastname: Watari
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Yagi
authors.forename: Takao
authors.initials: T
authors.lastname: Hamakubo
authors.forename: Masuo
authors.initials: M
authors.lastname: Kondoh
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000257:Adenoviridae Infections / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D045744:Cell Line, Tumor; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein / Q000235:genetics*; D005822:Genetic Vectors; D008156:Luciferases / Q000235:genetics / Q000378:metabolism*; D051379:Mice
chemical_list: D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D008156:Luciferases