title: The role of iron impurities in the toxic effects exerted by short multiwalled carbon nanotubes (MWCNT) in murine alveolar macrophages.
abstract: Lung toxicity mediated by multiwalled carbon nanotubes (MWCNT) has been widely demonstrated and recently associated with induction of carcinogenic asbestos-like effects, but the chemical features that drive this toxic effect have still not been well elucidated. The presence of metals as trace contaminants during MWCNT preparation, in particular iron (Fe) impurities, plays an important role in determining a different cellular response to MWCNT. Our goal was to clarify the mechanisms underlying MWCNT-induced toxicity with correlation to the presence of Fe impurities by exposing murine alveolar macrophages to two different MWCNT samples, which differed only in the presence or absence of Fe. Data showed that only Fe-rich MWCNT were significantly cytotoxic and genotoxic and induced a potent cellular oxidative stress, while Fe-free MWCNT did not exert any of these adverse effects. These results confirm that Fe content represents an important key constituent in promoting MWCNT-induced toxicity, and this needs to be taken into consideration when planning new, safer preparation routes.
authors.affiliation: a Department of Oncology , University of Torino , Torino , Italy.
authors.forename: E
authors.initials: E
authors.lastname: Aldieri
authors.forename: I
authors.initials: I
authors.lastname: Fenoglio
authors.forename: F
authors.initials: F
authors.lastname: Cesano
authors.forename: E
authors.initials: E
authors.lastname: Gazzano
authors.forename: G
authors.initials: G
authors.lastname: Gulino
authors.forename: D
authors.initials: D
authors.lastname: Scarano
authors.forename: A
authors.initials: A
authors.lastname: Attanasio
authors.forename: G
authors.initials: G
authors.lastname: Mazzucco
authors.forename: D
authors.initials: D
authors.lastname: Ghigo
authors.forename: B
authors.initials: B
authors.lastname: Fubini
mesh_terms: D000818:Animals; D002460:Cell Line; D020552:Comet Assay; D016166:Free Radical Scavengers; D018698:Glutamic Acid / Q000378:metabolism; D007501:Iron / Q000737:chemistry / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation; D016676:Macrophages, Alveolar / Q000187:drug effects*; D051379:Mice; D037742:Nanotubes, Carbon / Q000633:toxicity*; D017382:Reactive Oxygen Species
chemical_list: D016166:Free Radical Scavengers; D037742:Nanotubes, Carbon; D017382:Reactive Oxygen Species; D018698:Glutamic Acid; D007501:Iron; D007770:L-Lactate Dehydrogenase