title: Complete genome sequence of the hydrogenotrophic Archaeon Methanobacterium sp. Mb1 isolated from a production-scale biogas plant.
abstract: Methanobacterium sp. Mb1, a hydrogenotrophic methanogenic Archaeon, was isolated from a rural biogas plant producing methane-rich biogas from maize silage and cattle manure in Germany. Here we report the complete genome sequence of the novel methanogenic isolate Methanobacterium sp. Mb1 harboring a 2,029,766 bp circular chromosome featuring a GC content of 39.74%. The genome encodes two rRNA operons, 41 tRNA genes and 2021 coding sequences and represents the smallest genome currently known within the genus Methanobacterium.
authors.affiliation: Center for Biotechnology, Bielefeld University, D-33615 Bielefeld, Germany.
authors.forename: Irena
authors.initials: I
authors.lastname: Maus
authors.forename: Daniel
authors.initials: D
authors.lastname: Wibberg
authors.forename: Robbin
authors.initials: R
authors.lastname: Stantscheff
authors.forename: Katharina
authors.initials: K
authors.lastname: Cibis
authors.forename: Felix-Gregor
authors.initials: FG
authors.lastname: Eikmeyer
authors.forename: Helmut
authors.initials: H
authors.lastname: König
authors.forename: Alfred
authors.initials: A
authors.lastname: Pühler
authors.forename: Andreas
authors.initials: A
authors.lastname: Schlüter
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000382:microbiology; D020745:Genome, Archaeal / Q000235:genetics*; D008697:Methane / Q000378:metabolism; D017014:Methanobacterium / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D003313:Zea mays / Q000382:microbiology
chemical_list: D012336:RNA, Ribosomal, 16S; D008697:Methane
keywords: Biogas plant; Genome; High-throughput sequencing; Methanobacterium; Methanogenesis