title: Hsp70 positively regulates porcine circovirus type 2 replication in vitro.
abstract: The Hsp70 chaperone plays a central role in multiple processes within cells. Porcine circovirus type 2 (PCV2) is the essential causal agent of post-weaning multisystemic wasting syndrome (PMWS), which has spread worldwide. But the mechanism of PCV2 replication remains poorly understood. In this study, we firstly found the positive effect of heat stress on the replication of PCV2 in the continuous porcine monocytic cell line 3D4/31. Downregulation of Hsp70 by the specific chaperone inhibitor Quercetin or RNA interference and upregulation of Hsp70 by expression from a recombinant adenovirus showed that Hsp70 enhanced PCV2 genome replication and virion production. A specific interaction between Hsp70 and PCV2 Cap was confirmed by colocalization by confocal microscopy and co-immunoprecipitation. Furthermore, the NF-κB pathway was activated and caspase-3 activity was reduced when Hsp70 was overexpressed in PCV2-infected 3D4/31 cells. These data suggested that Hsp70 positively regulated PCV2 replication, which being helpful for understanding the molecular mechanism of PCV2 infection.
authors.affiliation: Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Juan
authors.initials: J
authors.lastname: Bai
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Jiang
authors.forename: Xianwei
authors.initials: X
authors.lastname: Wang
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002460:Cell Line; D017925:Circovirus / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D047468:Immunoprecipitation; D018613:Microscopy, Confocal; D025941:Protein Interaction Mapping; D013552:Swine; D015678:Viral Structural Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D018840:HSP70 Heat-Shock Proteins; D015678:Viral Structural Proteins
keywords: Caspase-3; Heat shock response; Hsp70; NF-κB; Porcine circovirus type 2