title: A highly sensitive and selective turn-on fluorogenic and colorimetric sensor based on pyrene-functionalized magnetic nanoparticles for Hg2+ detection and cell imaging.
abstract: In this paper, a colorimetric and "turn-on" fluorescent sensor (Py-Si-Fe3O4@SiO2 NPs) for Hg(2+) detection was designed with pyrene derivative covalently grafted onto the surface of magnetic core/shell Fe3O4@SiO2 nanoparticles using the silanol hydrolysis approach. The Py-Si-Fe3O4@SiO2 inorganic-organic hybrid material was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray power diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and fluorescence emission. The results of fluorescence spectra showed that the resultant multifunctional nanoparticles exhibited selective turn-on type fluorescence enhancement with Hg(2+). In addition, the presence of magnetic Fe3O4 nanoparticles in the sensor Py-Si-Fe3O4@SiO2 NPs would also facilitate the magnetic separation of Hg(2+)-Py-Si-Fe3O4@SiO2 from the solution. The as-prepared chemosensor was also successfully applied to detect Hg(2+) in environmental water samples and serum sample. Results from confocal laser scanning microscopy experiments demonstrated that this chemosensor was cell permeable and can be used as a fluorescent probe for monitoring Hg(2+) in living cells.
authors.affiliation: College of Chemistry, Sichuan University, No. 29 Wangjiang Road, Chengdu 610064, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Baozhan
authors.initials: B
authors.lastname: Zheng
authors.forename: Yong
authors.initials: Y
authors.lastname: Guo
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Dan
authors.initials: D
authors.lastname: Xiao
authors.forename: Lin
authors.initials: L
authors.lastname: Bo
mesh_terms: D001692:Biological Transport; D002413:Cations, Divalent; D005260:Female; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D005618:Fresh Water / Q000737:chemistry; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism / Q000648:ultrastructure*; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008628:Mercury / Q000032:analysis*; D057054:Molecular Imaging / Q000379:methods; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012821:Silanes / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D002413:Cations, Divalent; D058185:Magnetite Nanoparticles; D015335:Molecular Probes; D011721:Pyrenes; D012821:Silanes; C082343:silanol; D008628:Mercury; D052203:Ferrosoferric Oxide
keywords: Fluorescent sensor; Hg(2+); Living cells; Magnetic core/shell; Py–Si–Fe(3)O(4)@SiO(2) NPs; Water samples and serum sample