title: Simultaneous determination of cadaverine and putrescine using a disposable monoamine oxidase based biosensor.
abstract: The selective and simultaneous amperometric determination of putrescine (Put) and cadaverine (Cad) has been carried out using a novel design of screen-printed carbon electrode (SPCE) with two working electrodes connected in array mode. A mixture of 3% of tetrathiafulvalene (TTF), as mediator, and carbon ink was used for the construction of the screen-printed working electrode. The employment of different amounts of monoamine oxidase (MAO) enzyme on these modified TTF/SPCEs and the use of gold nanoparticles (AuNPs) allowed performing the simultaneous determination of both analytes. The amperometric detection has been performed by measuring the oxidation current of the mediator at a potential of+250 mV vs. screen-printed Ag/AgCl reference electrode. A linear response in the Cad concentration range from 19.6 till 107.1 µM and from 9.9 till 74.1 μM for Put was obtained at the MAO/AuNPs/TTF/SPCE biosensor. This device showed a capability of detection of 9.9 and 19.9±0.9 µM (n=4 α=β=0.05) and a precision of 4.9% and 10.3% in terms of relative standard deviation for Put and Cad, respectively. The developed biosensor was successfully applied to the simultaneous determination of Put and Cad in octopus samples.
authors.affiliation: Analytical Chemistry Department, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, Spain. Electronic address: whenao@ubu.es.
authors.forename: Wilder
authors.initials: W
authors.lastname: Henao-Escobar
authors.forename: Olga
authors.initials: O
authors.lastname: Domínguez-Renedo
authors.forename: M
authors.initials: M
authors.lastname: Asunción Alonso-Lomillo
authors.forename: M
authors.initials: M
authors.lastname: Julia Arcos-Martínez
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002103:Cadaverine / Q000032:analysis*; D002244:Carbon; D055664:Electrochemical Techniques; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D008995:Monoamine Oxidase / Q000737:chemistry*; D049831:Octopodiformes / Q000737:chemistry*; D010084:Oxidation-Reduction; D011700:Putrescine / Q000032:analysis*
chemical_list: D004800:Enzymes, Immobilized; D002244:Carbon; D006046:Gold; D008995:Monoamine Oxidase; D002103:Cadaverine; D011700:Putrescine
keywords: Cadaverine; Enzymatic amperometric biosensor; Monoamine oxidase; Putrescine; Screen-printed electrodes