title: Grain protein variability among populations of wild barley (Hordeum spontaneum C. Koch.) from Jordan.
abstract: Protein content, kernel weight, and genetic diversity in the storage protein hordein, encoded by the Hor 1 and Hor 2 loci, were assessed in 12 populations of wild barley (Hordeum spontaneum C. Koch.) collected from central, peripheral, and marginal areas of its distribution in Jordan. Protein content ranged from 106.3 to 239.1 g kg(-1), and kernel weight ranged from 21.17 to 31.8 mg. Populations with high protein content and heavy kernels have been identified. Electrophoretic analysis of the storage protein hordein showed that the two hordein loci, Hor 1 and Hor 2, are highly polymorphic, having 34 and 38 alleles, respectively. Polymorphism (He) was highest in central populations (He Hor 1=0.859, He Hor 2=0.782), intermediate in peripheral populations (He Hor 1=0.566, He Hor 2=0.509), and lowest in marginal populations (He Hor 1=0.392, He Hor 2=0.349). Geographical distances between populations were not indicative of Nei's genetic similarity (NI). NI values averaged 0.209 and ranged from 0.0 to 0.83, supporting the hypothesis of an island population model for the species. The high proportion of allelic diversity, apportioned among populations for Hor 1 (0.584) and Hor 2 (0.495) loci, indicates that these natural populations are a rich reserve of genetic variability for protein. This variability is readily exploitable in breeding.
authors.affiliation: Department of Agricultural Sciences, Jordan University of Science and Technology, P.O. Box 3030, Irbid, Jordan.
authors.forename: A A
authors.initials: AA
authors.lastname: Jaradat