title: Cockroach allergen Bla g 7 promotes TIM4 expression in dendritic cells leading to Th2 polarization.
abstract: As one of the most common sources of indoor aeroallergens worldwide, cockroach is important in causing rhinitis and asthma while the mechanisms underlying remain obscure. Since T helper (Th) type 2 polarization plays an important role in the pathogenesis of allergic diseases, we investigated the effect of Bla g 7, a pan-allergen from Blattella germanica (B. germanica), on Th polarization which is controlled by monocyte-derived dendritic cells (DCs). Challenged by recombinant Bla g 7 (rBla g 7), immature DCs obtained from human exhibited upregulated levels of TIM4, CD80, and CD86 and increased IL-13 secretion. Cocultured with CD4+ T cells, challenged DCs increased the ratio of IL-4+ versus IFN-γ+ of CD4+ T cells, suggesting a balance shift from Th1 to Th2. Moreover, antibodies against TIM4, CD80, and CD86 reversed the enhancement of IL-4+/IFN-γ+ ratio and alleviated the IL-13 release induced by rBla g 7, indicating that the Th2 polarization provoked by rBla g 7 challenged DCs is via TIM4-, CD80-, and CD86-dependent mechanisms. In conclusion, the present findings implied a crucial role of Bla g 7 in the development of cockroach allergy and highlighted an involvement of DCs-induced Th2 polarization in cockroach allergy.
authors.affiliation: Department of Rheumatology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China ; Clinical Research Centre, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
authors.forename: Lingxiao
authors.initials: L
authors.lastname: Xu
authors.forename: Miaojia
authors.initials: M
authors.lastname: Zhang
authors.forename: Wenjing
authors.initials: W
authors.lastname: Ma
authors.forename: Shanshan
authors.initials: S
authors.lastname: Jin
authors.forename: Weijuan
authors.initials: W
authors.lastname: Song
authors.forename: Shaoheng
authors.initials: S
authors.lastname: He
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D018793:Interleukin-13 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D009000:Monocytes / Q000166:cytology; D016328:NF-kappa B / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D015398:Signal Transduction; D018418:Th2 Cells / Q000378:metabolism*; D014335:Tropomyosin / Q000378:metabolism*
chemical_list: D000485:Allergens; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C481502:Bla g 7 allergen, Blattella germanica; D018793:Interleukin-13; D008565:Membrane Proteins; D016328:NF-kappa B; D011994:Recombinant Proteins; C512411:TIMD4 protein, human; D014335:Tropomyosin; D015847:Interleukin-4; D007371:Interferon-gamma