title: Effects of growth conditions of acetate utilization byRhodopseudomonas palustris isolated from a freshwater lake.
abstract: Rhodopseudomonas palustris, a purple non-sulfur bacterium, was recently found throughout the water column in Lake Kinneret. It was demonstrated to be of a versatile nature, growing under both aerobic and anaerobic conditions at different light intensities. A comparison of C-acetate uptake byR. palustris andChlorobium phaeobacterioides, a green sulfur bacterium, showed that, under identical growth conditions, C-acetate assimilation byR. palustris was greater. Furthermore, C-acetate uptake forR. palustris was greater than C-CO2 uptake at all light intensities. Depending on the prevailing conditions, acetate can be used byR. palustris as both an electron donor and carbon source. Malate synthase was used as an indicator of activity of the glyoxylic acid cycle. It was found that enzyme activity was higher (i.e., acetate was used mainly as a carbon source) under anaerobic conditions, in the dark, or in the absence of HCO3 (-). Acetate was used preferably as an electron donor under photosynthetic microaerophillic conditions.
authors.affiliation: The Yigal Allon, Kinneret Limnological Laboratory, P.O. Box 345, 14102, Tiberias, Israel.
authors.forename: B
authors.initials: B
authors.lastname: Butow
authors.forename: T B
authors.initials: TB
authors.lastname: Dan