title: Characterization of a pro-angiogenic, novel peptide from Russell's viper (Daboia russelii russelii) venom.
abstract: Present report shows for the first time on the induction of in vitro angiogenesis by a 3.9 kDa novel peptide (RVVAP) purified from Russell's viper venom. Secondary structure of RVVAP is made up of 36.8% α-helix, 33.3% β pleated sheets and 29.9% turns. Optimum angiogenesis and significant elevation in endothelial migration were observed at 50 ng/ml of RVVAP treatment; above this concentration, progressive decrease in wound healing was noted. RVVAP (1.0 μg/ml) was non-cytotoxic to U87-MG, HeLa and HT-29 cells; however, increasing the RVVAP concentration above 500 ng/ml resulted in induction of chromosomal aberrations and delay in cell cycle kinetics of Chinese hamster ovary cells.
authors.affiliation: Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, School of Science, Tezpur University, Tezpur 784028, India; School of Biological Sciences, University of Northern Colorado, Greeley, CO 80639, USA. Electronic address: akm@tezu.ernet.in.
authors.forename: Ashis K
authors.initials: AK
authors.lastname: Mukherjee
authors.affiliation: AU-KBC Research Centre, Anna University, Chennai 600044, India.
authors.forename: Suvro
authors.initials: S
authors.lastname: Chatterjee
authors.affiliation: AU-KBC Research Centre, Anna University, Chennai 600044, India.
authors.forename: Syamantak
authors.initials: S
authors.lastname: Majumder
authors.affiliation: Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, School of Science, Tezpur University, Tezpur 784028, India.
authors.forename: Debashree
authors.initials: D
authors.lastname: Saikia
authors.affiliation: Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, School of Science, Tezpur University, Tezpur 784028, India.
authors.forename: Rupamoni
authors.initials: R
authors.lastname: Thakur
authors.affiliation: Molecular Genetics Laboratory, Department of Biotechnology and Bioinformatics, North Eastern Hill University, Shillong 793022, India.
authors.forename: Anupam
authors.initials: A
authors.lastname: Chatterjee
mesh_terms: D042501:Angiogenic Proteins / Q000032:analysis* / Q000494:pharmacology*; D000818:Animals; D016466:CHO Cells; D002453:Cell Cycle / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects; D002645:Chickens; D002869:Chromosome Aberrations / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010063:Ovum / Q000187:drug effects; D011487:Protein Conformation; D017840:Daboia / Q000378:metabolism*; D014757:Viper Venoms / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D042501:Angiogenic Proteins; D014757:Viper Venoms
keywords: Angiogenesis; Chromosomal aberration; Cytotoxicity; Russell's viper; Wound healing