title: Plant regeneration from Carica protoplasts.
abstract: Rapidly proliferating and highly regenerable suspension cultures of somatic embryos of Carica papaya x C. cauliflora were used for protoplast isolation. On average, protoplast yield was 1.5×10(6)/g fresh weight of somatic embryos. Protoplasts were first cultured in liquid KM8P-S medium for 2 weeks and then plated in the same medium solidified with 1% agarose. About 1.4% of the protoplasts developed directly into somatic embryos. Protoplast-derived somatic embryos proliferated rapidly through direct embryogenesis on modified MS medium supplemented with 1 mg/1 ABA, and developed into plantlets upon transfer to MS medium devoid of plant growth regulators. The plantlets were successfully transplanted to soil.
authors.affiliation: Department of Botany, National Taiwan University, Taipei, Taiwan 107, Republic of China.
authors.forename: M H
authors.initials: MH
authors.lastname: Chen
authors.forename: C C
authors.initials: CC
authors.lastname: Chen