title: The presence of concanavalin A and canatoxin in Canavalia ensiformis DC tissue culture.
abstract: Isolated embryos, cotyledons and embryos plusa fragment of cotyledon from seeds of Canavalia ensiformis (jack bean) were cultured in vitro. Concanavalin A and canatoxin cross-reactive material were detected by double immunodiffusion tests. Canatoxin was detectable until 30 days in cultures of embryos, embryos plus cotyledons and hypocotyls. Concanavalin A was also present in all cultures being detected until 90 days in cultures treated with 6-benzylaminopurine. No concanavalin A was detected in root cultures. Concanavalin A was present in cell suspensions until 45 days of culture; the culture medium contained neither concanavalin A nor canatoxin. Tissue cultures thus can produce Con A and CNTX and will be an important research tool for studying the biosynthesis of such substances.
authors.affiliation: Laboratório de Fisiologia Vegetal, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, 21949-900, Rio de Janeiro, RJ, Brazil.
authors.forename: A
authors.initials: A
authors.lastname: Sato
authors.forename: G B
authors.initials: GB
authors.lastname: Barcellos
authors.forename: E C
authors.initials: EC
authors.lastname: Riedel
authors.forename: J A
authors.initials: JA
authors.lastname: Carneiro
authors.forename: C R
authors.initials: CR
authors.lastname: Carlini
authors.forename: M A
authors.initials: MA
authors.lastname: Esquibel