title: Effect of selenium nanoparticles with different sizes in primary cultured intestinal epithelial cells of crucian carp, Carassius auratus gibelio.
abstract: Nano-selenium (Se), with its high bioavailability and low toxicity, has attracted wide attention for its potential application in the prevention of oxidative damage in animal tissues. However, the effect of nano-Se of different sizes on the intestinal epithelial cells of the crucian carp (Carassius auratus gibelio) is poorly understood. Our study showed that different sizes and doses of nano-Se have varied effects on the cellular protein contents and the enzyme activities of secreted lactate dehydrogenase, intracellular sodium potassium adenosine triphosphatase, glutathione peroxidase, and superoxide dismutase. It was also indicated that nano-Se had a size-dependent effect on the primary intestinal epithelial cells of the crucian carp. Thus, these findings may bring us a step closer to understanding the size effect and the bioavailability of nano-Se on the intestinal tract of the crucian carp.
authors.affiliation: Marine Resources and Nutrition Biology Research Center, Food Quality and Safety Department, Zhejiang Gongshang University, Hangzhou, People's Republic of China.
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Wang
authors.forename: Xuxia
authors.initials: X
authors.lastname: Yan
authors.forename: Linglin
authors.initials: L
authors.lastname: Fu
mesh_terms: D000818:Animals; D002347:Carps; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012643:Selenium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D012643:Selenium
keywords: crucian carp; intestinal epithelial cell; primary culture; selenium nanoparticle