title: Use of RAPD markers to screen somatic hybrids between Solanum tuberosum and S. brevidens.
abstract: The identification of somatic hybrids between Solanum tuberosum and S. brevidens can be carried out using polymerase chain reaction (PCR) and arbitrary 10-mer primers to generate random amplified polymorphic DNA (RAPD) markers. Five commercial primers have been tested. Each primer directed the amplification of a genome-specific "fingerprint" for the fusion parents and S. brevidens. The size of the amplified DNA fragments ranged from 100 to 1800 base pairs. The somatic hybrids showed a combination of the parental banding profiles with four of the five primers surveyed, whereas regenerants from one of the parents had the same or a similar banding pattern to that of the parent. Thus RAPD markers provide a quick, simple and preliminary screening method for putative somatic hybrids.
authors.affiliation: Dept. of Crop Production, University of Helsinki, SF-00710, Helsinki, Finland.
authors.forename: Y S
authors.initials: YS
authors.lastname: Xu
authors.forename: M S
authors.initials: MS
authors.lastname: Clark
authors.forename: E
authors.initials: E
authors.lastname: Pehu