title: Genetic variation in Ameloblastin is associated with caries in asthmatic children.
abstract: OBJECTIVE
  Evidence suggests caries experience is higher in children with asthma. This study compared caries experience in asthmatic and non-asthmatic children and defined whether variation in the distribution of caries experience differed between the two groups and was dependent on the presence of genetic variation in enamel formation genes.
  
  
  METHODS
  Children with asthma were recruited at the Istanbul University, Faculty of Medicine, Department of Paediatrics, Division of Paediatric Allergy and Pulmonary Diseases, and non-affected children were recruited at the Istanbul University, Faculty of Dentistry, Department of Paedodontics. Cases (N = 100) were defined as children between the ages of 6 and 12 years with asthma and controls (N = 100) as children without asthma. Cases and controls were matched by sex and age. All study subjects received a complete dental exam, provided demographic and other caries and asthma risk factors data, and a saliva sample for DNA extraction. Caries experience was defined based on DMFT/dmft and DMFS/dmfs scores. Genotypes of 11 SNPs were selected in intronic regions of enamel development genes. PCR with TaqMan chemistry was used for genotyping all selected markers. Association between caries experience (caries-free versus caries affected) depending on asthma status and SNPs was tested with PLINK by logistic regression, adjusting by risk, and other preventive measures. p values below 0.0045 (0.05/11) were considered statistically significant.
  
  
  RESULTS
  Logistic regression analysis showed an association between AMBN rs4694075 and caries experience (p = 2.525e-007).
  
  
  CONCLUSIONS
  This study provides, for the first time, evidence that ameloblastin is associated with caries in asthmatic children.
authors.affiliation: Department of Pedodontics, Istanbul University, Istanbul, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Ergöz
authors.forename: F
authors.initials: F
authors.lastname: Seymen
authors.forename: K
authors.initials: K
authors.lastname: Gencay
authors.forename: Z
authors.initials: Z
authors.lastname: Tamay
authors.forename: K
authors.initials: K
authors.lastname: Deeley
authors.forename: S
authors.initials: S
authors.lastname: Vinski
authors.forename: A R
authors.initials: AR
authors.lastname: Vieira
mesh_terms: D000566:Amelogenesis / Q000235:genetics; D053523:Amelogenin / Q000235:genetics; D001249:Asthma / Q000150:complications*; D016022:Case-Control Studies; D002648:Child; D004245:DMF Index; D003731:Dental Caries / Q000150:complications / Q000235:genetics; D003746:Dental Enamel Proteins / Q000235:genetics*; D003774:Dental Plaque Index; D016326:Extracellular Matrix Proteins / Q000235:genetics; D005260:Female; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007438:Introns / Q000235:genetics; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D000072260:RNA Splicing Factors; D013941:Thymine; D014099:Toothbrushing
chemical_list: C490889:AMBN protein, human; D053523:Amelogenin; D003746:Dental Enamel Proteins; C550525:ENAM protein, human; D016326:Extracellular Matrix Proteins; D009687:Nuclear Proteins; D000072260:RNA Splicing Factors; C485860:TFIP11 protein, human; C038456:tuftelin; D013941:Thymine