title: Genotypic variability for protoplast regeneration in Saintpaulia ionantha (H. Wendl.).
abstract: The behaviour of eleven Saintpaulia ionantha (H. Wendl.) genotypes in protoplast culture was compared. Isolation of protoplasts from young shootlets regenerated in vitro on leaf explants, yielded 0.7 to 1.8 × 10(6) protoplasts per gram fresh weight. In all cultivars and breeding lines tested, cell divisions were observed. The mean division frequencies varied between 1.0 and 5.0% after 14 days, and between 6.4 and 13.8% after 24 days of culture. In ten genotypes callussing and shoot regeneration were achieved. The difference between the genotypes in shoot regeneration rate, between 2 and 68%, was more pronounced. The comparison of four cytokinins indicated hat thidiazuron was most effective for shoot regeneration, but often resulted in poorer shoot quality than benzylaminopurine.
authors.affiliation: Institute for Ornamental Plant Breeding, Bornkampsweg 31, D-22926, Ahrensberg, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Winkelmann
authors.forename: J
authors.initials: J
authors.lastname: Grunewaldt