title: Molecular characterization of recombinant mus a 5 allergen from banana fruit.
abstract: Allergy to banana fruit appears to have become an important cause of fruit allergy in Europe. Among five allergens that have been found, beta-1,3-glucanase denoted as Mus a 5 was identified as a candidate allergen for the component-resolved allergy diagnosis of banana allergy. Because of the variations in protein levels in banana fruit, in this study Mus a 5 was produced as a fusion protein with glutathione-S-transferase in Escherichia coli. The recombinant Mus a 5 was purified under native conditions by a combination of affinity, ion-exchange, and reversed phase chromatography. N-terminal sequence was confirmed by Edman degradation and 55 % of the primary structure was identified by mass fingerprint, while the secondary structure was assessed by circular dichroism spectroscopy. IgG reactivity of recombinant protein was shown in 2-D immunoblot with anti-Mus a 5 antibodies, while IgG and IgE binding to natural Mus a 5 was inhibited with the recombinant Mus a 5 in immunoblot inhibition test. IgE reactivity of recombinant Mus a 5 was shown in ELISA within a group of ten persons sensitized to banana fruit. Recombinant Mus a 5 is a novel reagent suitable for the component-resolved allergy diagnosis of banana allergy.
authors.affiliation: Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Studentski trg 16, 11000, Belgrade, Serbia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Mrkic
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abughren
authors.forename: Jasna
authors.initials: J
authors.lastname: Nikolic
authors.forename: Uros
authors.initials: U
authors.lastname: Andjelkovic
authors.forename: Emilia
authors.initials: E
authors.lastname: Vassilopoulou
authors.forename: Athanassios
authors.initials: A
authors.lastname: Sinaniotis
authors.forename: Arnd
authors.initials: A
authors.lastname: Petersen
authors.forename: Nikolaos G
authors.initials: NG
authors.lastname: Papadopoulos
authors.forename: Marija
authors.initials: M
authors.lastname: Gavrovic-Jankulovic
mesh_terms: D000485:Allergens / Q000235:genetics* / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D005060:Europe; D005512:Food Hypersensitivity / Q000235:genetics*; D005638:Fruit / Q000276:immunology; D043326:Glucan 1,3-beta-Glucosidase / Q000235:genetics* / Q000276:immunology; D006801:Humans; D051379:Mice; D028521:Musa; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000485:Allergens; D011994:Recombinant Proteins; D043326:Glucan 1,3-beta-Glucosidase