title: Genome-wide effects of DNA methyltransferase inhibitor on gene expression in double-stranded RNA transfected porcine PK15 cells.
abstract: Double-stranded RNA (dsRNA) is produced in host cells during viral replication. The effects of DNA demethylation on gene expression in dsRNA transfected swine cells are unclear. The study aims to profile the transcriptome changes which are induced by DNA methyltransferase inhibitor (Aza-CdR) in porcine PK15 cells transfected with viral-like dsRNA (Poly(I:C)). A total of 44, 76 and 952 differentially expressed genes (DEGs) were detected in the cells treated by Poly(I:C) plus Aza-CdR (P+A), Poly(I:C) (P) or Aza-CdR (A) alone compared to the controls (C). Immune response-related pathways are observed in the comparison of A vs. C and P vs. C, and the genes in the pathways were recovered in the comparison of (P+A) vs. C. GO analysis indicated that Aza-CdR has negative regulatory effects on viral reproduction. The results suggest that the stimulant of Poly(I:C) could be regressed by Aza-CdR. These observations provide new insights into the epigenetic regulatory effects on viral replication.
authors.affiliation: National Engineering Laboratory for Animal Breeding, Key Laboratory of Agricultural Animal Genetics and Breeding, Department of Animal Breeding and Genetics, College of Animal Sciences and Technology, China Agricultural University, Beijing 100193, China.
authors.forename: Xiaoshuo
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Animal Nutrition, Key Laboratory for Domestic Animal Genetic Resources and Breeding of the Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ao
authors.affiliation: National Engineering Laboratory for Animal Breeding, Key Laboratory of Agricultural Animal Genetics and Breeding, Department of Animal Breeding and Genetics, College of Animal Sciences and Technology, China Agricultural University, Beijing 100193, China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Zhai
authors.affiliation: State Key Laboratory for Animal Nutrition, Key Laboratory for Domestic Animal Genetic Resources and Breeding of the Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Lijing
authors.initials: L
authors.lastname: Bai
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Weiyong
authors.initials: W
authors.lastname: He
authors.affiliation: National Engineering Laboratory for Animal Breeding, Key Laboratory of Agricultural Animal Genetics and Breeding, Department of Animal Breeding and Genetics, College of Animal Sciences and Technology, China Agricultural University, Beijing 100193, China. Electronic address: yuying@cau.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yu
authors.affiliation: National Engineering Laboratory for Animal Breeding, Key Laboratory of Agricultural Animal Genetics and Breeding, Department of Animal Breeding and Genetics, College of Animal Sciences and Technology, China Agricultural University, Beijing 100193, China. Electronic address: cdwang@cau.edu.cn.
authors.forename: Chuduan
authors.initials: C
authors.lastname: Wang
mesh_terms: D000818:Animals; D001374:Azacitidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002460:Cell Line; D015254:DNA Modification Methylases / Q000037:antagonists & inhibitors*; D000077209:Decitabine; D016678:Genome; D016898:Interferon-alpha / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D011070:Poly I-C / Q000494:pharmacology*; D012330:RNA, Double-Stranded / Q000235:genetics*; D015398:Signal Transduction; D034421:Sus scrofa; D051196:Toll-Like Receptor 3 / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome / Q000187:drug effects*; D014162:Transfection
chemical_list: D016898:Interferon-alpha; D012330:RNA, Double-Stranded; D051196:Toll-Like Receptor 3; D000077209:Decitabine; D015254:DNA Modification Methylases; D001374:Azacitidine; D011070:Poly I-C
keywords: DNA demethylation; Double-stranded RNA; Transcriptome; Transfection; Virus replication