title: Mini-review: recent developments in hydroxynitrile lyases for industrial biotechnology.
abstract: Hydroxynitrile lyases (HNLs) catalyze the cleavage as well as the formation of cyanohydrins. The latter reaction is valuable for the stereoselective C-C bond formation by condensation of HCN with carbonyl compounds. The resulting cyanohydrins serve as versatile building blocks for a broad range of chemical and enzymatic follow-up reactions. A significant number of (R)- and (S)-selective HNLs are known today and the number is still increasing. HNLs not only exhibit varying substrate scope but also differ in sequence and structure. Tailor-made enzymes for large-scale manufacturing of cyanohydrins with improved yield and enantiomeric excess are very interesting targets, which is reflected in a solid number of patents. This review will complement and extend our recent review with a strong focus on applications of HNLs for the synthesis of highly functionalized, chiral compounds with newest literature, recent and current patent literature.
authors.forename: Elisa
authors.initials: E
authors.lastname: Lanfranchi
authors.forename: Kerstin
authors.initials: K
authors.lastname: Steiner
authors.forename: Anton
authors.initials: A
authors.lastname: Glieder
authors.forename: Ivan
authors.initials: I
authors.lastname: Hajnal
authors.forename: Roger A
authors.initials: RA
authors.lastname: Sheldon
authors.forename: Sander
authors.initials: S
authors.lastname: van Pelt
authors.affiliation: ACIB GmbH (Austrian Centre of Industrial Biotechnology GmbH), Petersgasse 14/III, A-8010 Graz, Austria. margit.winkler@acib.at.
authors.forename: Margit
authors.initials: M
authors.lastname: Winkler
mesh_terms: D000446:Aldehyde-Lyases / Q000235:genetics / Q000378:metabolism*; D001709:Biotechnology; D002384:Catalysis; D009570:Nitriles / Q000378:metabolism; D010330:Patents as Topic; D013237:Stereoisomerism
chemical_list: D009570:Nitriles; C430593:cyanohydrin; D000446:Aldehyde-Lyases; C021541:mandelonitrile lyase