title: Beta2-adrenergic receptor agonists reduce proliferation but not protein synthesis of periodontal fibroblasts stimulated with platelet-derived growth factor-BB.
abstract: OBJECTIVE
  Catecholamines released from β-adrenergic neurons upon stress can interfere with periodontal regeneration. The cellular mechanisms, however, are unclear. Here, we assessed the effect of catecholamines on proliferation of periodontal fibroblasts.
  
  
  METHODS
  Fibroblasts from the gingiva and the periodontal ligament were exposed to agonists of the β-adrenergic receptors; isoproterenol (ISO, non-selective β-adrenergic agonist), salbutamol (SAL, selective β2-adrenergic receptor agonist) and BRL 37344 (BRL selective β3-receptor agonist). Proliferation was stimulated with platelet-derived growth factor-BB (PDGF-BB). Pharmacological inhibitors and gene expression analysis further revealed β-adrenergic signalling.
  
  
  RESULTS
  Gingiva and periodontal ligament fibroblast express the β2-adrenergic receptor. ISO and SAL but not BRL decreased proliferation of fibroblasts in the presence of PDGF-BB. The inhibitory effect of β-adrenergic signalling on proliferation but not protein synthesis in response to PDGF-BB was reduced by propranolol, a non-selective β-adrenergic antagonist.
  
  
  CONCLUSIONS
  These results suggest that β2-receptor agonists can reduce the mitogenic response of periodontal fibroblasts. These data add to the compelling concept that blocking of β2-receptor signalling can support tissue maintenance and regeneration.
authors.affiliation: Department of Oral Surgery, Medical University of Vienna, Vienna, Austria; Austrian Cluster for Tissue Regeneration, Austria; Laboratory of Oral Cell Biology, School of Dental Medicine, University of Bern, Bern, Switzerland.
authors.forename: R
authors.initials: R
authors.lastname: Gruber
authors.forename: M
authors.initials: M
authors.lastname: Leimer
authors.forename: M B
authors.initials: MB
authors.lastname: Fischer
authors.forename: H
authors.initials: H
authors.lastname: Agis
mesh_terms: D058666:Adrenergic beta-2 Receptor Agonists / Q000494:pharmacology*; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000077214:Becaplermin; D002395:Catecholamines / Q000502:physiology; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects*; D005347:Fibroblasts / Q000378:metabolism*; D005562:Formazans; D005881:Gingiva / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007930:Leucine / Q000096:biosynthesis; D010513:Periodontal Ligament / Q000166:cytology* / Q000378:metabolism; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013778:Tetrazolium Salts
chemical_list: D058666:Adrenergic beta-2 Receptor Agonists; D000319:Adrenergic beta-Antagonists; D002395:Catecholamines; D005562:Formazans; D020574:Proto-Oncogene Proteins c-sis; D013778:Tetrazolium Salts; D000077214:Becaplermin; C079782:MTT formazan; D007930:Leucine
keywords: Beta2-adrenergic receptor; PDGF-BB; Periodontal therapy; Proliferation; Stress