title: Skeletal muscle satellite cell migration to injured tissue measured with 111In-oxine and high-resolution SPECT imaging.
abstract: The delivery of adult skeletal muscle stem cells, called satellite cells, to several injured muscles via the circulation would be useful, however, an improved understanding of cell fate and biodistribution following their delivery is important for this goal to be achieved. The objective of this study was to evaluate the ability of systemically delivered satellite cells to home to injured skeletal muscle using single-photon emission computed tomography (SPECT) imaging of (111)In-labeled satellite cells. Satellite cells labeled with (111)In-oxine and green fluorescent protein (GFP) were injected intravenously after bupivicaine-induced injury to the tibialis anterior muscle. Animals were imaged with a high-resolution SPECT system called FastSPECT II for up to 7 days after transplantation. In vivo FastSPECT II imaging demonstrated a three to five-fold greater number of transplanted satellite cells in bupivicaine-injured muscle as compared to un-injured muscle after transplantation; a finding that was verified through autoradiograph analysis and quantification of GFP expression. Satellite cells also accumulated in other organs including the lung, liver, and spleen, as determined by biodistribution measurements. These data support the ability of satellite cells to home to injured muscle and support the use of SPECT and autoradiograph imaging techniques to track systemically transplanted (111)In labeled satellite cells in vivo, and suggest their homing may be improved by reducing their entrapment in filter organs.
authors.affiliation: Muscle Biology Group, Department of Animal Sciences, University of Arizona, Tucson, AZ, USA.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Elster
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Rathbone
authors.forename: Zhonglin
authors.initials: Z
authors.lastname: Liu
authors.forename: Xiasong
authors.initials: X
authors.lastname: Liu
authors.forename: Harrison H
authors.initials: HH
authors.lastname: Barrett
authors.forename: Robert P
authors.initials: RP
authors.lastname: Rhoads
authors.forename: Ronald E
authors.initials: RE
authors.lastname: Allen
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D007205:Indium Radioisotopes; D008297:Male; D009942:Organometallic Compounds; D015125:Oxyquinoline / Q000031:analogs & derivatives*; D019275:Radiopharmaceuticals; D051381:Rats; D011916:Rats, Inbred F344; D017207:Rats, Sprague-Dawley; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000000981:diagnostic imaging*; D014018:Tissue Distribution; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods; D014162:Transfection
chemical_list: D007205:Indium Radioisotopes; D009942:Organometallic Compounds; D019275:Radiopharmaceuticals; C032950:indium oxine; D015125:Oxyquinoline