title: A novel proteoglycan from Ganoderma lucidum fruiting bodies protects kidney function and ameliorates diabetic nephropathy via its antioxidant activity in C57BL/6 db/db mice.
abstract: Diabetic nephropathy (DN) is the major cause of morbidity among diabetic patients. Thus, antidiabetic drugs with protection potential in the kidneys would have a higher therapeutic value. The effects of a novel proteoglycan, named FYGL, isolated from G. lucidum fruiting bodies, on the kidney function were investigated systematically in present work. FYGL (250 mg/kg) not only dosedependently reduced the blood glucose concentration (23.5%, p<0.05), kidney/body weight ratio (23.6%, p<0.01), serum creatinine (33.1%, p<0.01), urea nitrogen (24.1%, p<0.01),urea acid contents (35.9%, p<0.01) and albuminuria (30.7%, p<0.01)of DN mice compared to the untreated DN mice but also increased the renal superoxide dismutase (75.3%, p<0.01), glutathione peroxidase (35.0%, p<0.01) and catalase activities (58.5%, p<0.01) compared to the untreated DN mice. The decreasing of renal malondialdehyde content (34.3%, p<0.01) and 8-hydroxy-2'-deoxyguanosine expression (2.5-fold, p<0.01) were also observed in FYGL-treated DN mice compared to the untreated DN mice, along with an amelioration of renal morphologic abnormalities. We conclude that FYGL confers protection against the renal functional and morphologic injuries by increasing activities of antioxidants and inhibiting accumulation of oxidation, suggesting a potential nutritional supplement for the prevention and therapy of DN.
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, PR China.
authors.forename: Deng
authors.initials: D
authors.lastname: Pan
authors.affiliation: Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Pharmacy College, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, PR China.
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, PR China.
authors.forename: Congheng
authors.initials: C
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, PR China.
authors.forename: Zhixue
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, PR China. Electronic address: yanghongjie1964@yahoo.com.cn.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, PR China. Electronic address: pingzhou@fudan.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D003928:Diabetic Nephropathies / Q000503:physiopathology / Q000517:prevention & control*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D051379:Mice; D008810:Mice, Inbred C57BL; D011509:Proteoglycans / Q000302:isolation & purification / Q000494:pharmacology*; D020940:Reishi / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D011509:Proteoglycans; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: 8-Hydroxy-2′-deoxyguanosine (8-OHdG); 8-OHdG; 8-hydroxydeoxyguanosin; Antioxidant; BUN; CAT; Ccr; Creatinine clearance; DN; Diabetic nephropathy; FYGL; Fudan–Yueyang–G. lucidum; GSH-px; Ganoderma lucidum; HDL-c; LDL-c; MDA; Oxidative stress; Proteoglycan; ROS; SOD; Scr; TC; TG; UA; Ucr; blood urea nitrogen; catalase; diabetic nephropathy; glutathione peroxidase; high-density lipoprotein-cholesterol; low-density lipoprotein cholesterol; malondialdehyde; reactive oxygen species; serum creatinine; super oxide dismutase; total cholesterol; triacylglycerol; uric acid; urine creatinine