title: Embryogenesis and plant regeneration from isolated microspores of Brassica rapa L. ssp. Oleifera.
abstract: Conditions favourable to embryogenesis from isolated microspores of Brassica rapa L. ssp. oleifera (canola quality) were identified. A population with enhanced responsiveness for microspore embryogenesis (C200) was synthesized by crossing individual plants showing microspore embryogenic potential. For optimal microspore embryogenesis, buds (2-3mm in length, containing mid-late uninucieate microspores) were collected from older plants (2 months old) and microspores isolated and washed in iron-free B5 medium. NLN medium with its iron content reduced to half was beneficial for initial microspore culture. An elevated temperature(33-35°C) during the first day of culture, followed by maintenance at 25°C resulted in dozens of embryos from each isolation (about 100 buds). Seeds were obtained from plants regenerated from microsporederived embryos after colchicine treatment.
authors.affiliation: Allelix Crop Technologies (A division of Pioneer Hi-Bred Production Ltd), 6850 Goreway Dr., L4V 1P1, Mississauga, Ont., Canada.
authors.forename: L
authors.initials: L
authors.lastname: Burnett
authors.forename: S
authors.initials: S
authors.lastname: Yarrow
authors.forename: B
authors.initials: B
authors.lastname: Huang