title: Sortase A-mediated chemoenzymatic synthesis of complex glycosylphosphatidylinositol-anchored protein.
abstract: Green fluorescent protein and a glycosylphosphatidylinositol (GPI) anchor containing the common core structure and a lipid chain were synthesized and then coupled together in the promotion of bacterial sortase A (SrtA), which was the first example for the synthesis of a full-size GPI-anchored protein by SrtA, demonstrating that this can be a generally useful method for GPI-anchored protein synthesis.
authors.affiliation: Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, Michigan 48202, USA. zwguo@chem.wayne.edu.
authors.forename: Zhimeng
authors.initials: Z
authors.lastname: Wu
authors.forename: Xueqing
authors.initials: X
authors.lastname: Guo
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.forename: Zhongwu
authors.initials: Z
authors.lastname: Guo
mesh_terms: D000595:Amino Acid Sequence; D019881:Aminoacyltransferases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D017261:Glycosylphosphatidylinositols / Q000737:chemistry* / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D013211:Staphylococcus aureus / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D017261:Glycosylphosphatidylinositols; D008565:Membrane Proteins; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases