title: Effect of melatonin on kidney cold ischemic preservation injury.
abstract: Melatonin is a potent free radical scavenger of reactive oxygen species, nitric oxide synthase inhibitor and a well-known antioxidant secreted from pineal gland. This hormone has been reported to protect tissue from oxidative damage. In this study, we aim to investigate the effect of melatonin on kidney cold ischemia time when added to preservation solution. Thirty male Wistar albino rats were divided equally into three groups; Ringer Lactate (RL) solution, University of Wisconsin (UW) solution with and without melatonin. The serum Lactate Dehydrogenase (LDH) activities of the preservation solutions at 2(nd), 24(th), 36(th), and 48(th) hours were determined. Tissue malondialdehyde (MDA) levels were also measured and a histological examination was performed at 48(th) hour. Melatonin that added to preservation solution prevented enzyme elevation and decreased lipid peroxidation in preservation solution when compared to the control group (p<0.05). The histological examination revealed that UW solution containing melatonin significantly prevented the kidney from pathological injury (p<0.05). Melatonin added to preservation solutions such as UW solution seemed to protect the tissue preserved effectively from cold ischemic injury for up to 48 hour.
authors.affiliation: Department of General Surgery, Antalya Training and Research Hospital Antalya, Turkey.
authors.forename: Arif
authors.initials: A
authors.lastname: Aslaner
authors.forename: Omer
authors.initials: O
authors.lastname: Gunal
authors.forename: Hamdi Taner
authors.initials: HT
authors.lastname: Turgut
authors.forename: Erdal
authors.initials: E
authors.lastname: Celik
authors.forename: Umran
authors.initials: U
authors.lastname: Yildirim
authors.forename: Rojbin Karakoyun
authors.initials: RK
authors.lastname: Demirci
authors.forename: Umut Riza
authors.initials: UR
authors.lastname: Gunduz
authors.forename: Hasan
authors.initials: H
authors.lastname: Calis
authors.forename: Sami
authors.initials: S
authors.lastname: Dogan
keywords: Melatonin; University of Wisconsin solution; cold preservation; kidney