title: Vibrio vulnificus bacteriophage SSP002 as a possible biocontrol agent.
abstract: A novel Vibrio vulnificus-infecting bacteriophage, SSP002, belonging to the Siphoviridae family, was isolated from the coastal area of the Yellow Sea of South Korea. Host range analysis revealed that the growth inhibition of phage SSP002 is relatively specific to V. vulnificus strains from both clinical and environmental samples. In addition, a one-step growth curve analysis and a bacteriophage stability test revealed a latent period of 65 min, a burst size of 23 ± 2 PFU, as well as broad temperature (20°C to 60°C) and pH stability (pH 3 to 12) ranges. A Tn5 random transposon mutation of V. vulnificus and partial DNA sequencing of the inserted Tn5 regions revealed that the flhA, flhB, fliF, and fleQ mutants are resistant to SSP002 phage infection, suggesting that the flagellum may be the host receptor for infection. The subsequent construction of specific gene-inactivated mutants (flhA, flhB, fliF, and fleQ) and complementation experiments substantiated this. Previously, the genome of phage SSP002 was completely sequenced and analyzed. Comparative genomic analysis of phage SSP002 and Vibrio parahaemolyticus phage vB_VpaS_MAR10 showed differences among their tail-related genes, supporting different host ranges at the species level, even though their genome sequences are highly similar. An additional mouse survival test showed that the administration of phage SSP002 at a multiplicity of infection of 1,000 significantly protects mice from infection by V. vulnificus for up to 2 months, suggesting that this phage may be a good candidate for the development of biocontrol agents against V. vulnificus infection.
authors.affiliation: Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, and Center for Food and Bioconvergence, Seoul National University, Seoul, South Korea.
authors.forename: Hyun Sung
authors.initials: HS
authors.lastname: Lee
authors.forename: Slae
authors.initials: S
authors.lastname: Choi
authors.forename: Hakdong
authors.initials: H
authors.lastname: Shin
authors.forename: Ju-Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Choi
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*; D061046:Biological Control Agents; D004251:DNA Transposable Elements; D005816:Genetic Complementation Test; D016679:Genome, Viral; D058507:Host Specificity; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D009154:Mutation; D056910:Republic of Korea; D014735:Vibrio Infections / Q000382:microbiology / Q000401:mortality; D041261:Vibrio vulnificus / Q000472:pathogenicity / Q000821:virology*
chemical_list: D001426:Bacterial Proteins; D061046:Biological Control Agents; D004251:DNA Transposable Elements; C080688:FlhA protein, Bacteria; C075661:Flif protein, Bacteria; D008565:Membrane Proteins