title: A stable blue-light-derived signal modulates ultraviolet-light-induced activation of the chalcone-synthase gene in cultured parsley cells.
abstract: Run-off transcription assays were used to demonstrate that both the ultraviolet (UV)-B and blue-light receptors control transcription rates for chalcone-synthase mRNA in the course of light-induced flavonoid synthesis in parsley (Petroselinum crispum Miller (A.W. Hill)) cell-suspension cultures. Blue and red light alone, presumably acting via a blue-light receptor and active phytochrome (far-red absorbing form) respectively, can induce accumulation of chalcone-synthase mRNA. The extent of the response is however considerably smaller than that obtained when these wavebands are applied in combination with UV light. A preirradiation with blue light strongly increases the response to a subsequent UV pulse and this modulating effect of blue light is stable for at least 20 h. The modulating effect is abolished by a UV induction but can be reestablished by a second irradiation with blue light.
authors.affiliation: Institut für Biologie II/Botanik der Universität Freiburg, Schänzlestrasse 1, D-7800, Freiburg i.Br., Federal Republic of Germany.
authors.forename: S
authors.initials: S
authors.lastname: Ohl
authors.forename: K
authors.initials: K
authors.lastname: Hahlbrock
authors.forename: E
authors.initials: E
authors.lastname: Schäfer