title: Posttranscriptional heme control of catalase synthesis in the yeast Saccharomyces cerevisiae.
abstract: Compared to wild type cells, strains bearing the pleiotropic regulatory mutations cgr4 or cas1 synthesize apocatalase T at a high rate when grown on high glucose. Like heme-deficient ole3 single mutants, ole3 cgr4 and ole3 cas1 double mutants accumulate no catalase T protein in vivo. This defect introduced by the ole3 mutation is cured by the addition of ALA. By use of the inhibitor actinomycin D we confirm previous findings that ole3 mutants lack catalase T mRNA and show that (i) the ole3 cgr4 and ole3 cas1 double mutants do accumulate catalase T mRNA or mRNA precursor, and (ii) the processing or translation of this RNA or the accumulation of apocatalase T depends on the presence of home.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Rakowiecka 36, 02-532, Warsaw, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Sledziewski
authors.forename: J
authors.initials: J
authors.lastname: Rytka
authors.forename: T
authors.initials: T
authors.lastname: Biliński
authors.forename: H
authors.initials: H
authors.lastname: Hörtner
authors.forename: H
authors.initials: H
authors.lastname: Ruis