title: Peroxiredoxin I protein, a potential biomarker of hydronephrosis in fetal mice exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin.
abstract: OBJECTIVE
  In previous studies, we established an animal model of human congenital hydronephrosis with exposure of developing mice to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), but the etiopathogenesis is not entirely clear. The present study was to identify the changes that may be involved in the etiology at the protein level.
  
  
  METHODS
  C57BL/6J mice fetuses were treated with TCDD. Comparative proteomic analysis was adopted to identify the proteins associated with hydronephrosis induced by TCDD.
  
  
  RESULTS
  Two-dimensional electrophoresis display revealed that 19 protein spots were differentially expressed in the upper urinary tract tissues in fetal mice after exposure to TCDD. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) identified 12 up-regulated proteins: peroxiredoxin I (Prx I), cadherin 6, gamma-actin, radixin, desmin, type II transforming growth factor-beta receptor, chromogranin B, serum albumin precursor, transferrin, hypothetical protein LOC70984, lipk protein, and zinc finger protein 336. Histochemical staining indicated that Prx I protein was positively expressed in the ureteric epithelium in the treated group, and not in the control group, which is consistent with MALDI-TOF-MS.
  
  
  CONCLUSIONS
  Prx I protein may be a potential biomarker or responsive protein of hydronephrosis in fetal mice induced by TCDD.
authors.affiliation: Department of Pediatric Surgery, the First Affiliated Hospital of Xiamen University, 55 Zhenhai Road, Siming District, Xiamen 361003, China. Electronic address: llmx2001@tom.com.
authors.forename: Mingxue
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Laboratory Medicine, the First Affiliated Hospital of Xiamen University, Xiamen 361003, China. Electronic address: liujing_fairy@sina.com.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Urology, Children's Hospital, Chongqing Medical University, 136 Zhongshan 2nd Road, Yuzhong District, Chongqing 400014, China. Electronic address: liux_0217@163.com.
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Urology, Children's Hospital, Chongqing Medical University, 136 Zhongshan 2nd Road, Yuzhong District, Chongqing 400014, China. Electronic address: ghwei@mail.chcmu.com.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Wei
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D005315:Fetal Diseases / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D006869:Hydronephrosis / Q000139:chemically induced / Q000196:embryology / Q000378:metabolism*; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D054464:Peroxiredoxins / Q000378:metabolism*; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity
chemical_list: D015415:Biomarkers; D000072317:Polychlorinated Dibenzodioxins; D054464:Peroxiredoxins; C489899:Prdx1 protein, mouse
keywords: 2,3,7,8-Tetrachlorodibenzo-p-dioxin; Comparative proteomic analysis; Hydronephrosis; Prx I