title: Comparison of automated chemiluminescence immunoassays with capture enzyme immunoassays for the detection of measles and mumps IgM antibodies in serum.
abstract: Outbreaks of measles and mumps occur regularly in the UK. Rapid diagnosis of acute infection is important for both infection control and epidemiological purposes. The objective of this study was to compare the performance of an automated platform (DiaSorin Liaison(®), Saluggia, Italy) with a manual capture enzyme immunoassay (EIA; Microimmune, Hounslow, UK) for the detection of measles and mumps IgM antibodies in serum from symptomatic individuals. Ninety sera tested previously for measles (n=50) and mumps (n=40) IgM using the manual EIA were tested retrospectively using the DiaSorin Liaison(®) and the results compared. Sensitivity, specificity, inter-assay variability and intra-assay variability of the Liaison(®) assays were calculated. Sensitivity and specificity of the Liaison(®) assay for measles IgM were 92% and 100% respectively, with inter-assay variation of 14.1% and intra-assay variation of 12.5%. The sensitivity and specificity of the mumps IgM Liaison(®) assay were 88% and 95% respectively, with an inter-assay and intra-assay variation of 13.9% and 5.3% respectively. Both the measles and mumps IgM Liaison(®) assays gave fewer equivocal results than the EIA. Neither Liaison(®) IgM assay showed any cross-reactivity with sera positive against other viruses, however the measles IgM EIA cross-reacted with parvovirus IgM. The automated Liaison(®) assays are more specific, cheaper and less labour-intensive compared to the manual EIA. The Liaison(®) assays benefit from reduced number of equivocal results compared to the EIA for both measles and mumps IgM. This allows clinical decisions to be made accurately and in a timely manner.
authors.affiliation: Department of Virology, Royal Free London NHS Foundation Trust, London, UK. Electronic address: becky.haywood@phe.gov.uk.
authors.forename: Becky
authors.initials: B
authors.lastname: Haywood
authors.affiliation: Department of Virology, Royal Free London NHS Foundation Trust, London, UK.
authors.forename: Mauli
authors.initials: M
authors.lastname: Patel
authors.affiliation: Department of Virology, Royal Free London NHS Foundation Trust, London, UK.
authors.forename: Samantha
authors.initials: S
authors.lastname: Hurday
authors.affiliation: Department of Virology, Royal Free London NHS Foundation Trust, London, UK.
authors.forename: Ruth
authors.initials: R
authors.lastname: Copping
authors.affiliation: Department of Virology, Royal Free London NHS Foundation Trust, London, UK.
authors.forename: Daniel
authors.initials: D
authors.lastname: Webster
authors.affiliation: Department of Virology, Royal Free London NHS Foundation Trust, London, UK.
authors.forename: Dianne
authors.initials: D
authors.lastname: Irish
authors.affiliation: Department of Virology, Royal Free London NHS Foundation Trust, London, UK.
authors.forename: Tanzina
authors.initials: T
authors.lastname: Haque
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D057205:Automation, Laboratory / Q000379:methods*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007075:Immunoglobulin M / Q000097:blood*; D008457:Measles / Q000175:diagnosis* / Q000276:immunology; D009107:Mumps / Q000175:diagnosis* / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D044967:Serum / Q000276:immunology; D006113:United Kingdom
chemical_list: D000914:Antibodies, Viral; D007075:Immunoglobulin M
keywords: Immunoassay; Measles; Mumps