title: Poly(2 deoxy 2 methacrylamido glucopyranose)bPoly(methacrylate amine)s: Optimization of Diblock Glycopol ycations for Nucleic Acid Delivery.
abstract: A series of nine poly(2-deoxy-2-methacrylamido glucopyranose)-b-poly(methacrylate amine) diblock copolycations The cationic block was varied in length and in the degree of methyl group substitution (secondary, tertiary, quaternary) on the pendant amine in an effort to optimize the structure and activity for plasmid DNA delivery. Upon a thorough kinetic study of polymerization for each polymer, the glycopolymers were prepared with well-controlled Mnand Ð. The binding and colloidal stability of the polymer-pDNA nanocomplexes at different N/P ratios and in biological media has been investigated using gel electrophoresis and light scattering techniques. The toxicity and transfection efficiency of the polyplexes has been evaluated with Hep G2 (human liver hepatocellular carcinoma) cells; several polymers displayed excellent delivery and toxicity profiles justifying their further development forin vivogene therapy.
authors.affiliation: Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, MN 55455, United States.
authors.forename: Haibo
authors.initials: H
authors.lastname: Li
authors.forename: Mallory A
authors.initials: MA
authors.lastname: Cortez
authors.forename: Haley R
authors.initials: HR
authors.lastname: Phillips
authors.forename: Yaoying
authors.initials: Y
authors.lastname: Wu
authors.forename: Theresa M
authors.initials: TM
authors.lastname: Reineke