title: Electrochemical determination of cefotaxime based on a three-dimensional molecularly imprinted film sensor.
abstract: A novel electrochemical sensor is presented for the determination of cefotaxime (CEF), which is constructed by molecularly imprinted polymer (MIP), gold networks@IL (IL, 1-butyl-3-methylimidazolium tetrafluoroborate, [BMIM][BF4]) (GNWs@IL), porous platinum nanoparticles (PPNPs) and carboxyl graphene (COOH-r-GO). The GNWs@IL is prepared by directly reducing HAuCl4 with sodium citrate in [BMIM][BF4] aqueous solution. The PPNPs are well embedded in GNWs@IL due to the adhesion of IL to form GNWs@IL-PPNPs suspension, which is coated on a COOH-r-GO modified glassy carbon electrode to construct a porous three-dimensional networks modified electrode. Then, MIP is prepared by cyclic voltammetry at the modified electrode, using CEF as template and o-phenylenediamine as monomer. The factors concerning this assay strategy are carefully investigated. Under the optimal conditions, the electrochemical sensor offers an excellent response for CEF, the linear response range is 3.9 × 10(-9) ~ 8.9 × 10(-6) mol L(-1) and the detection limit is 1.0 × 10(-10) mol L(-1). The electrochemical sensor has been applied to the determination of CEF in real samples with satisfactory results.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China; Department of Resources and Environment, Baoshan University, Baoshan 678000, PR China.
authors.forename: Guangming
authors.initials: G
authors.lastname: Yang
authors.forename: Faqiong
authors.initials: F
authors.lastname: Zhao
authors.forename: Baizhao
authors.initials: B
authors.lastname: Zeng
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002439:Cefotaxime / Q000737:chemistry / Q000302:isolation & purification*; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D054802:Molecular Imprinting; D010984:Platinum / Q000737:chemistry; D011108:Polymers / Q000737:chemistry
chemical_list: D011108:Polymers; D010984:Platinum; D006046:Gold; D006108:Graphite; D002439:Cefotaxime
keywords: Cefotaxime; Electrochemical sensor; Gold networks; Ionic liquid; Molecular imprinted polymers; Porous platinum nanoparticles