title: Characterization of a cDNA encoding 5-aminolevulinic acid dehydratase in tomato (Lycopersicon esculentum Mill.).
abstract: A full-length cDNA clone encoding tomato (Lycopersicon esculentum Mill.) 5-aminolevulinic acid dehydratase (ALAD) was isolated and characterized. The primary structure predicts a 430-amino acid precursor which comprises a 41.7 kDa, 388-amino acid mature protein and a 47-amino acid transit sequence. The tomato primary sequence shows extensive homology to those of pea and spinach. Southern analysis indicated that 1 to 2 copies of the ALAD gene are present in the tomato genome. Northern blot analysis shows differential expression in various tomato organs, and constitutive developmental expression in tomato fruits.
authors.affiliation: Department of Horticulture and Interdepartmental Plant Physiology Major, Iowa State University, 50011, Ames, IA, USA.
authors.forename: G F
authors.initials: GF
authors.lastname: Polking
authors.forename: D J
authors.initials: DJ
authors.lastname: Hannapel
authors.forename: R J
authors.initials: RJ
authors.lastname: Gladon