title: Effect of insulin-like growth factor-1 on corneal surface ultrastructure and nerve regeneration of rabbit eyes after laser in situ keratomileusis.
abstract: To explore the effect of insulin-like growth factor-1 (IGF-1) on corneal surface ultrastructure and nerve regeneration in rabbit models after laser in situ keratomileusis (LASIK). Forty-two healthy New Zealand white rabbits were divided into two groups, the IGF-1 group and the control group, and LASIK surgery was performed. The corneal surface ultrastructure was observed by transmission electron microscopy, and the nerve regeneration was evaluated by counting the newly regenerated nerves at 1 d, 1 w, 2 w, 1 m, 3 m and 6 m after surgery. Dry eye parameters, including the Schirmer I test and tear break-up time, were examined at all time points. The examination of corneal ultrastructure showed that the number of corneal epithelial microvilli in the IGF-1 group was significantly higher than that in the normal saline (NS) group except in the second postoperative week (p<0.05). The observation of corneal nerve regeneration showed that the number of regenerated nerve fibers in the IGF-1 group was higher than the control group at all time points (p<0.05). The parameters of dry eye were significantly higher in the IGF-1 group compared to the control group at all time points except at 1d and 6m after LASIK. IGF-1 can effectively accelerate the early repair of corneal surface ultrastructure and nerve regeneration after LASIK and relieve dry eye symptoms in rabbit eyes.
authors.affiliation: The Department of Ophthalmology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China. Electronic address: wangchunyanzeng@126.com.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Wang
authors.affiliation: The Department of Ophthalmology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China. Electronic address: liyanli62@aliyun.com.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Peng
authors.affiliation: The Department of Ophthalmology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
authors.forename: Shuling
authors.initials: S
authors.lastname: Pan
authors.affiliation: The Department of Ophthalmology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China. Electronic address: zenglingboai@126.com.
authors.forename: Li
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D003315:Cornea / Q000187:drug effects* / Q000294:innervation* / Q000601:surgery / Q000648:ultrastructure; D015352:Dry Eye Syndromes / Q000188:drug therapy / Q000209:etiology; D019573:Epithelium, Corneal / Q000187:drug effects / Q000294:innervation / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage / Q000494:pharmacology*; D020731:Keratomileusis, Laser In Situ / Q000009:adverse effects; D008297:Male; D009416:Nerve Regeneration / Q000187:drug effects*; D009883:Ophthalmic Solutions; D011208:Powders; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D009883:Ophthalmic Solutions; D011208:Powders; D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I
keywords: Corneal ultrastructure; Dry eye; Insulin-like growth factor-1; Laser in situ keratomileusis; Nerve regeneration