title: [Recombinant human IL-16 inhibits proliferation and induces apoptosis in MT-4 cells].
abstract: OBJECTIVE
  To investigate the effects of recombinant human IL-16 (rhIL-16) on the proliferation and apoptosis of MT-4 cells.
  
  
  METHODS
  MT-4 cells were infected with adenovirus expressing rhIL-16 at different time points. Then the proliferation of MT-4 cells was measured by the MTT assay. The apoptosis of the MT-4 cells was evaluated by flow cytometry. The expression of two molecular markers for apoptosis, Bax and Bcl-2, were analyzed by Western blotting.
  
  
  RESULTS
  The MTT assay results showed that pAd-IL-16 transfectants had inhibitory effect on the proliferation of MT-4 cells, compared with untreated cells at various time points. Flow cytometry revealed that apoptotic rate of MT-4 cells was higher than that of control group. Furthermore, Western blotting indicated that the Bax protein expression increased while Bcl-2 protein expression decreased in a time-dependent manner in MT-4 cells infected with the rhIL-17-expressing adenovirus.
  
  
  CONCLUSIONS
  These results demonstrated that IL-16 plays an important role in the proliferation and apoptosis of MT-4 cells, and the molecular mechanism underlying this phenomenon may involve the modulation of Bax and Bcl-2 expression.
authors.affiliation: Research Center for Immunology, Xinxiang Medical University, Xinxiang 453003, China.
authors.forename: Moli
authors.initials: M
authors.lastname: Zhu
authors.forename: Haiyan
authors.initials: H
authors.lastname: Song
authors.forename: Guizeng
authors.initials: G
authors.lastname: Zhao
authors.forename: Yaping
authors.initials: Y
authors.lastname: Wu
authors.forename: Nana
authors.initials: N
authors.lastname: Li
authors.forename: Zhitao
authors.initials: Z
authors.lastname: Gao
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D019410:Interleukin-16 / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D019410:Interleukin-16; D019253:Proto-Oncogene Proteins c-bcl-2; D011994:Recombinant Proteins; D051028:bcl-2-Associated X Protein