title: Characterization of polyphosphate glucokinase SCO5059 from Streptomyces coelicolor A3(2).
abstract: SCO5059, encoded in Streptomyces coelicolor A3(2), was identified as a polyphosphate glucokinase. The Km values of SCO5059 for glucose and polyphosphate (poly(P)6) were estimated to be 12 and 4 µM, respectively, and the kcat value was 0.3 s(-1) at pH 7.7 at 28 °C. SCO5059 homologs are highly conserved among Streptomyces, and can work as polyphosphate glucokinase as well.
authors.affiliation: Department of Chemistry and Materials Science, Tokyo Institute of Technology.
authors.forename: Mai
authors.initials: M
authors.lastname: Koide
authors.forename: Akimasa
authors.initials: A
authors.lastname: Miyanaga
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Kudo
authors.forename: Tadashi
authors.initials: T
authors.lastname: Eguchi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010770:Phosphotransferases / Q000235:genetics / Q000378:metabolism*; D011122:Polyphosphates / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D048372:Streptomyces coelicolor / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D011122:Polyphosphates; D011994:Recombinant Proteins; D010770:Phosphotransferases; C024812:polyphosphate-glucose phosphotransferase; D005947:Glucose