title: Leaf morphoanatomy of species tolerant to excess iron and evaluation of their phytoextraction potential.
abstract: Setaria parviflora (Poir.) Kerguélen and Paspalum urvillei Steudel are grasses that grow naturally in a soil with high iron contents. This study aimed to characterize morphoanatomically and histochemically the iron phytotoxicity on leaves and evaluate the phytoextraction potential of these grasses. Saplings were cultivated in hydroponic solution with and without excess Fe-EDTA. Regarding measurements taken on leaves, reduction was observed among treatments of Fe-EDTA on height values of abaxial epidermis and bundle sheath in both species. As for iron histolocalization, stronger reaction was observed in leaves of S. parviflora, in comparison with P. urvillei. Anatomical damage, such as protoplast retraction, irregular xylem, changes in cell volume, and cell collapse, and visual symptoms, like leaf bronzing, chlorosis, and necrosis, were similar in both species when exposed to excess iron; however, P. urvillei showed more severe damage. This species accumulated more iron in shoots than S. parviflora and therefore is more favorable for use in phytoextraction. The root system of both species accumulated higher iron concentrations in relation to shoots.
authors.affiliation: Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil.
authors.forename: Brenda Vila Nova
authors.initials: BV
authors.lastname: Santana
authors.forename: Talita Oliveira
authors.initials: TO
authors.lastname: de Araújo
authors.forename: Guilherme Carvalho
authors.initials: GC
authors.lastname: Andrade
authors.forename: Larisse
authors.initials: L
authors.lastname: de Freitas-Silva
authors.forename: Kacilda Naomi
authors.initials: KN
authors.lastname: Kuki
authors.forename: Eduardo Gusmão
authors.initials: EG
authors.lastname: Pereira
authors.forename: Aristéa Alves
authors.initials: AA
authors.lastname: Azevedo
authors.forename: Luzimar Campos
authors.initials: LC
authors.lastname: da Silva
mesh_terms: D001673:Biodegradation, Environmental; D007501:Iron / Q000378:metabolism / Q000633:toxicity*; D031781:Paspalum / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism; D018515:Plant Leaves / Q000033:anatomy & histology / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D018520:Plant Shoots / Q000378:metabolism; D032983:Setaria Plant / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D007501:Iron