title: A putative Rab-GTPase activation protein from Nicotiana benthamiana is important for Bamboo mosaic virus intercellular movement.
abstract: The cDNA-amplified fragment length polymorphism technique was applied to isolate the differentially expressed genes during Bamboo mosaic virus (BaMV) infection on Nicotiana benthamiana plants. One of the upregulated genes was cloned and predicted to contain a TBC domain designated as NbRabGAP1 (Rab GTPase activation protein 1). No significant difference was observed in BaMV accumulation in the NbRabGAP1-knockdown and the control protoplasts. However, BaMV accumulation was 50% and 2% in the inoculated and systemic leaves, respectively, of the knockdown plants to those of the control plants. By measuring the spreading area of BaMV infection foci in the inoculated leaves, we found that BaMV moved less efficiently in the NbRabGAP1-knockdown plants than in the control plants. Transient expression of the wild type NbRabGAP1 significantly increases BaMV accumulation in N. benthamiana. These results suggest that NbRabGAP1 with a functional Rab-GAP activity is involved in virus movement.
authors.affiliation: Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Ying-Ping
authors.initials: YP
authors.lastname: Huang
authors.forename: Jao-Shien
authors.initials: JS
authors.lastname: Chen
authors.forename: Yau-Huei
authors.initials: YH
authors.lastname: Hsu
authors.forename: Ching-Hsiu
authors.initials: CH
authors.lastname: Tsai
mesh_terms: D055785:Gene Knockdown Techniques; D005816:Genetic Complementation Test; D054884:Host-Pathogen Interactions; D018515:Plant Leaves / Q000821:virology; D017863:Potexvirus / Q000472:pathogenicity*; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics / Q000821:virology*; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D020691:rab GTP-Binding Proteins
keywords: Bamboo mosaic virus; CMV; PVX; Rab-GAP; Systemic movement; TBC domain; Virus movement