title: Somatic embryogenesis and plant regeneration in Acanthopanax senticosus.
abstract: Mature embryos of Acanthopanax senticosus explanted on Murashige and Skoog (MS) medium with 0.5 mg/1 2,4-D developed somatic embryos directly from swollen cotyledon and embryo axes within one to two months. When the somatic embryos were transferred to medium supplemented with 2,4-D (0.5 mg/1) or IAA (1-3 mg/1) or Zeatin (0.5 mg/1) and NAA (0.2 mg/1), additional somatic embryos developed. Most (93%) embryos germinated on the above medium without 2,4-D. Sixty-two percent of the plantlets survived in soil. Histological observations revealed that the somatic embryos originated from cell masses of epidermal and sub-epidermal origin. There was no cytological separation zone between the somatic embryos and cultured expiants. Consequently, embryos were difficult to separate from their expiant tissue.
authors.affiliation: Institute of Botany, Academia Sinica, 100044, Beijing, Peoples Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Gui
authors.forename: Z
authors.initials: Z
authors.lastname: Guo
authors.forename: S
authors.initials: S
authors.lastname: Ke
authors.forename: R M
authors.initials: RM
authors.lastname: Skirvin