title: Hephaestia caeni gen. nov., sp. nov., a novel member of the family Sphingomonadaceae isolated from activated sludge.
abstract: A Gram-staining-negative, rod-shaped and motile bacterium, designated strain ERB1-3(T), was isolated from a laboratory-scale activated sludge system treating coke plant effluent using thiocyanate-supplemented growth medium. Strain ERB1-3(T) was oxidase-positive and weakly catalase-positive. The predominant fatty acids were C18:1ω7c (35.6 %) and C17:1ω6c (29.2%), and the major respiratory quinone was Q-10. Polar lipids were dominated by sphingoglycolipid and phosphatidylglycerol. Major polyamines were spermidine and sym-homospermidine. The G+C content of the genomic DNA of strain ERB1-3(T) was 66.4 mol%. Based on the 16S rRNA gene, strain ERB1-3(T) exhibited the highest sequence similarity values to Sphingomonas sanxanigenens DSM 19645(T) (96.1%), Sphingobium scionense DSM 19371(T) (95.1%) and Stakelama pacifica LMG 24686(T) (94.8%) within the family Sphingomonadaceae. The novel isolate had some unique chemotaxonomic features that differentiated it from these closely related strains, contained much more C17 : 1ω6c, C15 : 0 2-OH, C17:0 and C17:1ω8c fatty acids and possessed diphosphatidylglycerol only in trace amounts. On the basis of the phenotypic, chemotaxonomic and molecular data, strain ERB1-3(T) is considered to represent a novel genus and species, for which the name Hephaestia caeni gen. nov., sp. nov. is proposed. The type strain is ERB1-3(T) ( = DSM 25527(T) = NCAIM B 02511(T)).
authors.affiliation: Department of Microbiology, Eötvös Loránd University, Pázmány Péter stny. 1/c, H-1117 Budapest, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Felföldi
authors.affiliation: Department of Microbiology, Eötvös Loránd University, Pázmány Péter stny. 1/c, H-1117 Budapest, Hungary.
authors.forename: Anita
authors.initials: A
authors.lastname: Vengring
authors.affiliation: Department of Microbiology, Eötvös Loránd University, Pázmány Péter stny. 1/c, H-1117 Budapest, Hungary.
authors.forename: Károly
authors.initials: K
authors.lastname: Márialigeti
authors.affiliation: Department of Microbiology, Eötvös Loránd University, Pázmány Péter stny. 1/c, H-1117 Budapest, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: András
authors.affiliation: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.affiliation: Department of Microbiology, Eötvös Loránd University, Pázmány Péter stny. 1/c, H-1117 Budapest, Hungary.
authors.forename: Erika M
authors.initials: EM
authors.lastname: Tóth
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006814:Hungary; D008969:Molecular Sequence Data; D010715:Phosphatidylglycerols / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D013095:Spermidine / Q000031:analogs & derivatives / Q000737:chemistry; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010715:Phosphatidylglycerols; D012336:RNA, Ribosomal, 16S; D012722:Sewage; C007961:sym-homospermidine; D014451:Ubiquinone; C025204:Ubiquinone Q2; D013095:Spermidine