title: GmFNSII-controlled soybean flavone metabolism responds to abiotic stresses and regulates plant salt tolerance.
abstract: Flavones, a major group of flavonoids in most plant tissues, play multiple roles in plant-environment interactions. In our study, the expression of the two soybean flavone synthase genes, GmFNSII-1 and GmFNSII-2, was significantly increased by methyl jasmonate (MeJA), glucose, mannitol and NaCl treatment, which were also found to increase flavone aglycone accumulation in Glycine max (L.) Merrill. In the GmFNSII-1 promoter, a specific CGTCA motif in the region (-979 bp to -806 bp) involved in the MeJA response was identified. Promoter deletion analysis of GmFNSII-2 revealed the presence of osmotic-responsive (-1,143 bp to -767 bp) and glucose-repressive sequence elements (-767 bp to -475 bp), which strongly supported the hypothesis that glucose induces soybean flavone production by acting as both an osmotic factor and a sugar signaling molecule simultaneously. Silencing of the GmFNSII gene clearly reduced the production of flavone aglycones (apigenin, luteolin and 7,4'-dihydroxyflavone) in hairy roots. The GmFNSII-RNAi (RNA interference) roots that had a reduced level of flavones accompanied by more malondialdehyde and H2O2 accumulation were more sensitive to salt stress compared with those of the control, and we concluded that flavones, as antioxidants, are associated with salt tolerance.
authors.affiliation: Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, No. 800 Dongchuan Rd., Shanghai, PR China 200240.
authors.forename: Junhui
authors.initials: J
authors.lastname: Yan
authors.forename: Biao
authors.initials: B
authors.lastname: Wang
authors.forename: Yina
authors.initials: Y
authors.lastname: Jiang
authors.forename: Linjing
authors.initials: L
authors.lastname: Cheng
authors.forename: Tianlong
authors.initials: T
authors.lastname: Wu
mesh_terms: D000085:Acetates / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology; D047309:Flavones / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D017930:Genes, Reporter; D005947:Glucose / Q000494:pharmacology; D005966:Glucuronidase / Q000378:metabolism; D008353:Mannitol / Q000494:pharmacology; D059372:Nucleotide Motifs / Q000235:genetics; D016877:Oxidants / Q000378:metabolism; D054883:Oxylipins / Q000494:pharmacology; D010545:Peroxides / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D055049:Salt Tolerance / Q000187:drug effects / Q000235:genetics; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D017384:Sequence Deletion / Q000235:genetics; D030262:Soybean Proteins / Q000235:genetics / Q000378:metabolism*; D013025:Glycine max / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D047309:Flavones; D016877:Oxidants; D054883:Oxylipins; D010545:Peroxides; D030262:Soybean Proteins; D008353:Mannitol; C072239:methyl jasmonate; D005966:Glucuronidase; D005947:Glucose; C043562:flavone
keywords: Flavone aglycones; Glucose; Methyl jasmonate; Promoter deletion analysis; Salt tolerance; Soybean flavone synthase genes