title: Panton-Valentine leukocidin (PVL) gene carriage among Staphylococcus aureus strains with SCCmec types I, III, IV, and V recovered from cystic fibrosis pediatric patients in Brazil.
abstract: The prevalence of methicillin-resistant Staphylococcus aureus (MRSA) is increasing in patients with cystic fibrosis (CF). We report a molecular characterization, antimicrobial resistance, and Panton-Valentine leukocidin (PVL) toxin gene detection of MRSA strains from 28 Brazilian pediatric CF patients (1 strain per patient). A significant proportion (50%) of MRSA SCCmec IV isolates was observed. Nearly half of MRSA strains harboring the PVL genes distributed in all SCCmec types detected. Multilocus sequence typing (MLST) analyses showed majority (57.1%) of the isolates belonged to known epidemic lineages, such as UK/EMRSA-3, Pediatric/USA 800, Southwest Pacific clone, and Brazilian/Hungarian clone. To our knowledge, this is the first Brazilian study of molecular epidemiology based on MLST and SCCmec typing and the first description of PVL genes in MRSA from CF patients.
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, fundos, 3º andar - Vila Isabel, Rio de Janeiro 20551-030, Brazil.
authors.forename: Danielle Ferreira
authors.initials: DF
authors.lastname: Lima
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, fundos, 3º andar - Vila Isabel, Rio de Janeiro 20551-030, Brazil.
authors.forename: Nathalia Brito Veloso
authors.initials: NB
authors.lastname: Brazão
authors.affiliation: Instituto Fernandes Figueira, Fundação Instituto Oswaldo Cruz, Avenida Rui Barbosa, 716 - Flamengo, Rio de Janeiro 22250-020, Brazil.
authors.forename: Tania Wrobel
authors.initials: TW
authors.lastname: Folescu
authors.affiliation: Departamento Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Rua Professor Ernani Melo, 101 Niterói, Rio Janiero, 24210-130, Brazil.
authors.forename: Felipe Piedade
authors.initials: FP
authors.lastname: Neves
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, fundos, 3º andar - Vila Isabel, Rio de Janeiro 20551-030, Brazil.
authors.forename: Alex Guerra
authors.initials: AG
authors.lastname: Ferreira
authors.affiliation: Laboratório de Bacteriologia, Hospital Universitário Pedro Ernesto, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, Vila Isabel, Rio de Janeiro 20551-030, Brazil.
authors.forename: Erica Aparecida
authors.initials: EA
authors.lastname: Santos
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, fundos, 3º andar - Vila Isabel, Rio de Janeiro 20551-030, Brazil; Laboratório de Bacteriologia, Hospital Universitário Pedro Ernesto, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, Vila Isabel, Rio de Janeiro 20551-030, Brazil.
authors.forename: Elizabeth Andrade
authors.initials: EA
authors.lastname: Marques
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, fundos, 3º andar - Vila Isabel, Rio de Janeiro 20551-030, Brazil. Electronic address: robson.leao@uerj.br.
authors.forename: Robson Souza
authors.initials: RS
authors.lastname: Leão
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics*; D001938:Brazil / Q000453:epidemiology; D003550:Cystic Fibrosis / Q000150:complications*; D005098:Exotoxins / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007956:Leukocidins / Q000235:genetics*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification*; D017720:Molecular Epidemiology; D058889:Molecular Typing; D015995:Prevalence; D013203:Staphylococcal Infections / Q000382:microbiology*; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D037521:Virulence Factors
keywords: Cystic fibrosis; MLST; MRSA; PVL; SCCmec; Staphylococcus aureus