title: Licochalcone A inhibiting proliferation of bladder cancer T24 cells by inducing reactive oxygen species production.
abstract: The aim of this study was to determine the relationship between proliferation inhibition and the production of reactive oxygen species (ROS) induced by Licochalcone A (LCA). Cell viability was evaluated using sulforhodamine B (SRB) assay. Intracellular ROS level was assessed using the 2, 7-dichlorofluorescein diacetate (H2DCFDA) probe and dihydroethidium (DHE) probe assay. The results indicate that LCA inhibits human bladder cancer T24 proliferation in a concentration-dependent manner, with an IC50 value of approximately 55 μM. The LCA-induced ROS production is inhibited by the co-treatment of LCA and free radical scavenger N-acetyl-cysteine (NAC), on the contrary, the proliferation rate and ROS production increase when treated by the combination of LCA and L-buthionine-(S,R)-sulfoximine (BSO). The ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG) decreases in a concentration-dependent manner. The results suggest that LCA inhibits proliferation by increasing intracellular ROS levels resulted in an oxidative stress status in T24 cells.
authors.affiliation: School of Pharmacy, Shihezi University, Shihezi, 832002, Xinjiang, China.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Jiang
authors.forename: Xuan
authors.initials: X
authors.lastname: Yuan
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
authors.forename: Xinyan
authors.initials: X
authors.lastname: Yan
authors.forename: Xiling
authors.initials: X
authors.lastname: Sun
authors.forename: Qiusheng
authors.initials: Q
authors.lastname: Zheng
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D047188:Chalcones / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005452:Fluoresceins / Q000737:chemistry; D005978:Glutathione / Q000378:metabolism; D006035:Glycyrrhiza / Q000737:chemistry; D006801:Humans; D018384:Oxidative Stress; D010936:Plant Extracts / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012235:Rhodamines / Q000737:chemistry; D001749:Urinary Bladder Neoplasms / Q000473:pathology*
chemical_list: D047188:Chalcones; D005452:Fluoresceins; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D012235:Rhodamines; C029569:diacetyldichlorofluorescein; C022027:lissamine rhodamine B; D005978:Glutathione; C070840:licochalcone A
keywords: Cell proliferation; GSH; GSSG; Licochalcone A; ROS; T24 cells