title: [Knockout of the ptsG gene in engineered Escherichia coli for homoethanol fermentation from sugar mixture].
abstract: To realize the simultaneous fermentation of xylose and glucose, ptsG (one of the glucose-PTS genes) was deleted from the engineered ethanologenic Escherichia coli SZ470 (deltapflB, deltafrdABCD, deltaackA, deltaldhA), resulting in loss of glucose effect in the mutant SZ470P (deltaptsG). When tested in 5% mixture of glucose (2.5%) and xylose (2.5%), SZ470P simultaneously used glucose (13 g/L) and xylose (20 g/L) whereas the parent strain SZ470 sequentially used glucose (25 g/L) then xylose (5 g/L). Upon completion of the fermentation, both strains achieved similar product yield of 89%. SZ470P produced 15.01 g/L of ethanol, which was 14.32% higher than that produced by SZ470 (12.86 g/L). Deleting ptsG gene enabled the mutant strain SZ470P to simultaneously use both glucose and xylose and achieve better ethanol production.
authors.affiliation: College of Bioengineering, Hubei University of Technology, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan 430068, Hubei, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yan
authors.forename: Jinfang
authors.initials: J
authors.lastname: Zhao
authors.forename: Wenhui
authors.initials: W
authors.lastname: Gao
authors.forename: Jinhua
authors.initials: J
authors.lastname: Wang
authors.forename: Yongze
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhao
authors.forename: Shengde
authors.initials: S
authors.lastname: Zhou
mesh_terms: D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D000431:Ethanol / Q000737:chemistry*; D005285:Fermentation; D005947:Glucose / Q000737:chemistry; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics*; D014994:Xylose / Q000737:chemistry
chemical_list: D000431:Ethanol; D014994:Xylose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C031629:phosphoenolpyruvate-glucose phosphotransferase; D005947:Glucose