title: Design, synthesis, and biological evaluation of Erythrina alkaloid analogues as neuronal nicotinic acetylcholine receptor antagonists.
abstract: The synthesis of a new series of Erythrina alkaloid analogues and their pharmacological characterization at various nicotine acetylcholine receptor (nAChR) subtypes are described. The compounds were designed to be simplified analogues of aromatic erythrinanes with the aim of obtaining subtype-selective antagonists for the nAChRs and thereby probe the potential of using these natural products as scaffolds for further ligand optimization. The most selective and potent nAChR ligand to come from the series, 6,7-dimethoxy-2-methyl-1,2,3,4-tetrahydroisoquinoline (3c) (also a natural product by the name of O-methylcorypalline), displayed submicromolar binding affinity toward the α4β2 nAChR with more than 300-fold selectivity over α4β4, α3β4, and α7. Furthermore, this lead structure (which also has inhibitory activity at monoamine oxidases A and B and at the serotonin and norepinephrine transporters) showed antidepressant-like effect in the mouse forced swim test at 30 mg/kg.
authors.affiliation: Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen , Universitetsparken 2, 2100 Copenhagen, Denmark.
authors.forename: François
authors.initials: F
authors.lastname: Crestey
authors.forename: Anders A
authors.initials: AA
authors.lastname: Jensen
authors.forename: Morten
authors.initials: M
authors.lastname: Borch
authors.forename: Jesper Tobias
authors.initials: JT
authors.lastname: Andreasen
authors.forename: Jacob
authors.initials: J
authors.lastname: Andersen
authors.forename: Thomas
authors.initials: T
authors.lastname: Balle
authors.forename: Jesper Langgaard
authors.initials: JL
authors.lastname: Kristensen
mesh_terms: D000470:Alkaloids / Q000138:chemical synthesis / Q000494:pharmacology; D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology; D019556:COS Cells; D002522:Chlorocebus aethiops; D004895:Erythrina / Q000737:chemistry; D051379:Mice; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D018733:Nicotinic Antagonists / Q000138:chemical synthesis* / Q000494:pharmacology; D011978:Receptors, Nicotinic / Q000378:metabolism; D013329:Structure-Activity Relationship; D013550:Swimming; D044005:Tetrahydroisoquinolines / Q000138:chemical synthesis* / Q000494:pharmacology
chemical_list: D000470:Alkaloids; D000928:Antidepressive Agents; D008996:Monoamine Oxidase Inhibitors; D018733:Nicotinic Antagonists; D011978:Receptors, Nicotinic; D044005:Tetrahydroisoquinolines