title: Advances in nanomaterial-based microwaves and infrared wave-assisted tryptic digestion for ultrafast proteolysis and rapid detection by MALDI-MS.
abstract: The unique physical/chemical properties of nanomaterials have significant impacts in electromagnetic waves (microwave and infrared waves)-assisted tryptic digestion approaches by using them as heat absorbers to expedite digestion and as affinity probes to enrich digested proteins prior to MALDI-MS analysis. We review recent developments in electromagnetic waves (microwaves and infrared waves)-assisted proteolysis using nanomaterials as heat absorbers and as affinity probes for analysis of digested proteins in MALDI-MS. New trends in ultrafast proteolysis (nonphosphoproteins- lysozyme, cytochrome c, myoglobin and bovine serum albumin (BSA); phosphoproteins- α- and β- caseins) using nanomaterials based microwaves and infrared (IR) waves assisted digestion approaches for rapid identification of digested proteins in the MALDI-MS.
authors.forename: Suresh Kumar
authors.initials: SK
authors.lastname: Kailasa
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 80424, Taiwan. hwu@faculty.nsysu.edu.tw.
authors.forename: Hui-Fen
authors.initials: HF
authors.lastname: Wu
mesh_terms: D000818:Animals; D002364:Caseins / Q000737:chemistry; D002417:Cattle; D045304:Cytochromes c / Q000737:chemistry; D006801:Humans; D007259:Infrared Rays; D008872:Microwaves; D009113:Muramidase / Q000737:chemistry; D009211:Myoglobin / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D010446:Peptide Fragments / Q000032:analysis; D010750:Phosphoproteins / Q000737:chemistry; D059748:Proteolysis / Q000528:radiation effects*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014357:Trypsin / Q000737:chemistry
chemical_list: D002364:Caseins; D009211:Myoglobin; D010446:Peptide Fragments; D010750:Phosphoproteins; D012710:Serum Albumin, Bovine; D045304:Cytochromes c; D009113:Muramidase; D014357:Trypsin