title: The effects of cadmium chloride and sodium selenite on protein synthesis in mouse liver.
abstract: The study aimed at evaluating the effects of cadmium and selenite ions on protein synthesis and metallothioneins content in mice liver after 2 h, 8 h, 24 h and 14 days of exposure. Our studies revealed that cadmium suppressed protein synthesis after 2 h and 24 h, but activated after 8h and 14 days. Also, the endogenous mRNA translation were reduced under any exposure to cadmium, meanwhile, metallothioneins content was decreased after 2 h, but then was progressively increasing up to 492% after 14 days. Meantime, selenite did not influence metallothioneins content, caused mild activation of protein synthesis, and slightly suppressed the endogenous mRNA translation. The combined treatments with cadmium and selenite favored toward resisting of protein synthesis to cadmium after 2 h and 24 h of intoxication. Besides, selenite also protected translation against cadmium in cell-free systems, but did not attenuate effects of cadmium on metallothioneins content.
authors.affiliation: Neuroscience Institute, Medical Academy, Lithuanian University of Health Sciences, Eiveniu 4, LT-50009 Kaunas, Lithuania. Electronic address: rasgai@med.kmu.lt.
authors.forename: Rasa
authors.initials: R
authors.lastname: Bernotiene
authors.forename: Laima
authors.initials: L
authors.lastname: Ivanoviene
authors.forename: Ilona
authors.initials: I
authors.lastname: Sadauskiene
authors.forename: Arunas
authors.initials: A
authors.lastname: Liekis
authors.forename: Leonid
authors.initials: L
authors.lastname: Ivanov
mesh_terms: D000818:Animals; D019256:Cadmium Chloride / Q000633:toxicity*; D002474:Cell-Free System; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008668:Metallothionein / Q000235:genetics / Q000378:metabolism; D051379:Mice; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D018038:Sodium Selenite / Q000633:toxicity*
chemical_list: D012333:RNA, Messenger; D008668:Metallothionein; D018038:Sodium Selenite; D019256:Cadmium Chloride
keywords: Cadmium; Metallothioneins; Protein synthesis; Selenium