title: Proteomic analysis of testicular ischemia-reperfusion injury in rats.
abstract: Testicular torsion is a urological emergency that leads to serious testicular damage and male infertility. We performed this study to identify specific proteins that are differentially expressed in response to testicular torsion and detorsion-induced ischemia-reperfusion (I-R) injury. Adult male rats were divided into two groups: a sham-operated group and a testicular I-R group. Testicular torsion was induced by rotating the left testis 720° in a clockwise direction for 1 hr, and then, detorsion was performed for 24 hr. After this testicular tissues were collected, protein analysis was performed using two-dimensional gel electrophoresis and Western blot analyses. Testicular I-R injury resulted in serious histopathologic damage to the germinal cells in the seminiferous tubules and increased the number of TUNEL-positive cells in testicular tissue. Specific protein spots with a greater than 2.5-fold change in intensity between the sham-operated and testicular I-R groups were identified by mass spectrometry. Among these proteins, levels of peroxiredoxin 6, thioredoxin, heterogeneous nuclear ribonucleoproteins, ubiquitin carboxyl terminal hydrolase isozyme L5 and zinc finger AN1-type domain 3 were decreased in the testicular I-R group compared to the sham-operated group. Moreover, Western blot analysis clearly showed the decrease of these proteins in the testicular I-R group. These proteins have spermatogenesis and anti-oxidative functions. These findings suggest that testicular I-R results in cell death due to altered expression of several proteins with spermatogenesis and anti-oxidation functions.
authors.affiliation: Department of Anatomy, College of Veterinary Medicine and Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea.
authors.forename: In-Ohk
authors.initials: IO
authors.lastname: Ouh
authors.forename: Min-Goo
authors.initials: MG
authors.lastname: Seo
authors.forename: Fawad-Ali
authors.initials: FA
authors.lastname: Shah
authors.forename: Sang-Ah
authors.initials: SA
authors.lastname: Gim
authors.forename: Phil-Ok
authors.initials: PO
authors.lastname: Koh
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D008297:Male; D013058:Mass Spectrometry; D054465:Peroxiredoxin VI / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D051381:Rats; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D013086:Spermatic Cord Torsion / Q000150:complications*; D013879:Thioredoxins / Q000378:metabolism; D043222:Ubiquitin Thiolesterase / Q000378:metabolism
chemical_list: D034441:Heterogeneous-Nuclear Ribonucleoproteins; D013879:Thioredoxins; D054465:Peroxiredoxin VI; D043222:Ubiquitin Thiolesterase