title: Quality control of a molybdoenzyme by the Lon protease.
abstract: Molybdoenzymes contain a molybdenum cofactor in their active site to catalyze various redox reactions in all domains of life. To decipher crucial steps during their biogenesis, the TorA molybdoenzyme of Escherichia coli had played a major role to understand molybdoenzyme maturation process driven by specific chaperones. TorD, the specific chaperone of TorA, is also involved in TorA protection. Here, we show that immature TorA (apoTorA) is degraded in vivo and in vitro by the Lon protease. Lon interacts with apoTorA but not with holoTorA. Lon and TorD compete for apoTorA binding but TorD binding protects apoTorA against degradation. Lon is the first protease shown to eliminate an immature or misfolded molybdoenzyme probably by targeting its inactive catalytic site.
authors.affiliation: Laboratoire de Chimie Bactérienne, Institut de Microbiologie de la Méditerranée, Centre National de la Recherche Scientifique, Aix-Marseille Université, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
authors.forename: David
authors.initials: D
authors.lastname: Redelberger
authors.forename: Olivier
authors.initials: O
authors.lastname: Genest
authors.forename: Dallel
authors.initials: D
authors.lastname: Arabet
authors.forename: Vincent
authors.initials: V
authors.lastname: Méjean
authors.forename: Marianne
authors.initials: M
authors.lastname: Ilbert
authors.forename: Chantal
authors.initials: C
authors.lastname: Iobbi-Nivol
mesh_terms: D003067:Coenzymes / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism* / Q000502:physiology; D008667:Metalloproteins / Q000235:genetics / Q000378:metabolism*; D018832:Molecular Chaperones / Q000502:physiology; D000090006:Molybdenum Cofactors; D010089:Oxidoreductases, N-Demethylating / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D049070:Protease La / Q000378:metabolism*; D011485:Protein Binding; D011499:Protein Processing, Post-Translational / Q000235:genetics; D059748:Proteolysis; D011621:Pteridines / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D003067:Coenzymes; D029968:Escherichia coli Proteins; D008667:Metalloproteins; D018832:Molecular Chaperones; D000090006:Molybdenum Cofactors; D010089:Oxidoreductases, N-Demethylating; D049070:Protease La; D011621:Pteridines; C088083:TorD protein, E coli; C022261:molybdopterin; C023186:trimethylamine dehydrogenase; C483567:Lon protein, E coli
keywords: Chaperone; Microbiology; Protease; Protein degradation; Protein–protein interaction