title: Differential miRNA expression in repeated recurrence of nasopharyngeal carcinoma.
abstract: Deregulated microRNAs (miRNAs) are known to be involved in the tumorigenesis of nasopharyngeal carcinoma (NPC). However, the role of miRNA expression in tumor recurrence is not yet understood. We found distinctive miRNA expression in repeated recurrent tumors using miRNA microarray, and verified this using quantitative real-time RT-PCR and miRNA in situ hybridization analysis. Computational analysis and immunohistochemistry further revealed that differentially expressed miRNAs may work in concert to modulate a multitude of biological pathways. The results not only indicate differential miRNA expression during tumor relapse, but imply the potential use of miRNAs to classify repeated recurrence of NPC beyond the histological approach.
authors.affiliation: Institute of Medical Sciences, Tzu-Chi University, Hualien, Taiwan.
authors.forename: Ling-Jung
authors.initials: LJ
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology, Buddhist Tzu Chi General Hospital, Hualien, Taiwan.
authors.forename: Yu-Fu
authors.initials: YF
authors.lastname: Chou
authors.affiliation: Department of Otolaryngology, Buddhist Tzu Chi General Hospital, Hualien, Taiwan.
authors.forename: Peir-Rong
authors.initials: PR
authors.lastname: Chen
authors.affiliation: Department of Pathology, Buddhist Tzu Chi General Hospital, Hualien, Taiwan.
authors.forename: Borcherng
authors.initials: B
authors.lastname: Su
authors.affiliation: Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan, Taiwan; Center for Nanotechnology, Chung Yuan Christian University, Taoyuan, Taiwan.
authors.forename: Yih-Chih
authors.initials: YC
authors.lastname: Hsu
authors.affiliation: Research Center of Excellence for Environment Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Department of Dermatology, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Chung-Hsing
authors.initials: CH
authors.lastname: Chang
authors.affiliation: Institute of Medical Sciences, Tzu-Chi University, Hualien, Taiwan; Department of Life Sciences, Tzu-Chi University, Hualien, Taiwan. Electronic address: jwlee@mail.tcu.edu.tw.
authors.forename: Jeng-Woei
authors.initials: JW
authors.lastname: Lee
mesh_terms: D002277:Carcinoma; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000378:metabolism; D009364:Neoplasm Recurrence, Local / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs
keywords: MicroRNA; Nasopharyngeal carcinoma; Recurrence