title: Dynamic changes of Foxp3(+) regulatory T cells in spleen and brain of canine distemper virus-infected dogs.
abstract: Canine distemper virus (CDV) infection causes immunosuppression and demyelinating leukoencephalitis in dogs. In viral diseases, an ambiguous function of regulatory T cells (Treg), with both beneficial effects by reducing immunopathology and detrimental effects by inhibiting antiviral immunity, has been described. However, the role of Treg in the pathogenesis of canine distemper remains unknown. In order to determine the effect of CDV upon immune homeostasis, the amount of Foxp3(+) Treg in spleen and brain of naturally infected dogs has been determined by immunohistochemistry. In addition, splenic cytokine expression has been quantified by reverse transcriptase polymerase chain reaction. Splenic depletion of Foxp3(+) Treg was associated with an increased mRNA-expression of tumor necrosis factor and decreased transcription of interleukin-2 in the acute disease phase, indicative of disturbed immunological counter regulation in peripheral lymphoid organs. In the brain, a lack of Foxp3(+) Treg in predemyelinating and early demyelinating lesions and significantly increased infiltrations of Foxp3(+) Treg in chronic demyelinating lesions were observed. In conclusion, disturbed peripheral and CNS immune regulation associated with a reduction of Treg represents a potential prerequisite for excessive neuroinflammation and early lesion development in canine distemper leukoencephalitis.
authors.affiliation: Department of Pathology, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany; Center for Systems Neuroscience, Hannover, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Qeska
authors.forename: Y
authors.initials: Y
authors.lastname: Barthel
authors.forename: M
authors.initials: M
authors.lastname: Iseringhausen
authors.forename: A
authors.initials: A
authors.lastname: Tipold
authors.forename: V M
authors.initials: VM
authors.lastname: Stein
authors.forename: M A
authors.initials: MA
authors.lastname: Khan
authors.forename: W
authors.initials: W
authors.lastname: Baumgärtner
authors.forename: A
authors.initials: A
authors.lastname: Beineke
mesh_terms: D000818:Animals; D001921:Brain / Q000276:immunology*; D004216:Distemper / Q000276:immunology*; D004285:Dogs; D051858:Forkhead Transcription Factors / Q000032:analysis; D013154:Spleen / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D051858:Forkhead Transcription Factors
keywords: Canine distemper virus; Cytokine expression; Demyelinating leukoencephalitis; Foxp3; Immunopathology; Regulatory T cells