title: Electrochemical real-time nucleic acid amplification: towards point-of-care quantification of pathogens.
abstract: Real-time nucleic acid amplification, whereby the amplification rate is used to quantify the initial copy number of target DNA or RNA, has proven highly effective for monitoring pathogen loads. Unfortunately, however, current optical methods are limited to centralized laboratories due to complexity, bulk and cost. In response, recent efforts aim to develop lower-cost, electrochemical real-time amplification platforms for point-of-care applications, with researchers already having developed platforms that not only perform in situ and concurrent electrochemical detection during amplification, but also deliver sensitivity and specificity potentially rivaling bench-top optical systems. This report chronicles the evolution of the different strategies, describes the current state of the art, and identifies challenges of bringing the power of real-time detection to the point-of-care.
authors.affiliation: Interdepartmental Program in Biomolecular Science and Engineering, University of California, Santa Barbara, Santa Barbara, CA, 93106, USA.
authors.forename: Adriana S
authors.initials: AS
authors.lastname: Patterson
authors.forename: Kuangwen
authors.initials: K
authors.lastname: Hsieh
authors.forename: H Tom
authors.initials: HT
authors.lastname: Soh
authors.forename: Kevin W
authors.initials: KW
authors.lastname: Plaxco
mesh_terms: D021141:Nucleic Acid Amplification Techniques; D019095:Point-of-Care Systems; D060888:Real-Time Polymerase Chain Reaction
keywords: electrochemistry; loop-mediated isothermal amplification; pathogen detection; point-of-care; quantitative polymerase chain reaction; real-time amplification