title: P-class pentatricopeptide repeat proteins are required for efficient 5' end formation of plant mitochondrial transcripts.
abstract: It is well recognized that flowering plants maintain a particularly broad spectrum of factors to support gene expression in mitochondria. Many of these factors are pentatricopeptide repeat (PPR) proteins that participate in virtually all processes dealing with RNA. One of these processes is the post-transcriptional generation of mature 5' termini of RNA. Several PPR proteins are required for efficient 5' maturation of mitochondrial mRNA and rRNA. These so-called RNA PROCESSING FACTORs (RPF) exclusively represent P-class PPR proteins, mainly composed of canonical PPR motifs without any extra domains. Applying the recent PPR-nucleotide recognition code, binding sites of RPF are predicted on the 5' leader sequences. The sequence-specific interaction of an RPF with one or a few RNA substrates probably directly or indirectly recruits an as-yet-unidentified endonuclease to the processing site(s). The identification and characterization of RPF is a major step toward the understanding of the role of 5' end maturation in flowering plant mitochondria.
authors.affiliation: Institut Molekulare Botanik; Universität Ulm; Ulm; Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Binder
authors.affiliation: Institut Molekulare Botanik; Universität Ulm; Ulm; Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Stoll
authors.affiliation: Institut Molekulare Botanik; Universität Ulm; Ulm; Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Stoll
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D042967:Electron Transport Complex I / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000235:genetics*; D051479:Plant Infertility / Q000235:genetics; D012323:RNA Processing, Post-Transcriptional; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism; D000077278:RNA, Mitochondrial; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D012333:RNA, Messenger; D000077278:RNA, Mitochondrial; D018749:RNA, Plant; D016601:RNA-Binding Proteins; C000597733:RPF1 protein, Arabidopsis; C000597734:RPF2 protein, Arabidopsis; C029305:mitochondrial messenger RNA; C513204:NAD4 protein, Arabidopsis; D042967:Electron Transport Complex I
keywords: Arabidopsis thaliana; RESTORER OF FERTILITY-like proteins; RNA PROCCESSING FACTORS; endonucleolytic processing; pentatricopeptide repeat proteins; post-transcriptional 5′ maturation