title: Physical map of the COB region in mitochondrial DNA of Saccharomyces cerevisiae.
abstract: A physical map of the COB region in mtDNA of yeast has been established. This region harbours a split gene coding for apocytochrome b. It includes restriction sites of seven endonucleases (EcoRI, HindII, HindIII, HaeIII, HpaII, AluI and BamHI). Various mtDNA sequences of this region, retained in a series of genetically characterized rho(-) clones have been allocated to this map. The combination of this physical map with a genetic map of the rho(-) clones revealed that 1) cob(-) mutational sites spread over 8,400 bp, 2) mutations in sequences coding for apocytochrome b map in five distinct segments which are separated by intervening sequences with minimum lengths from 350 to 1,900 bp.
authors.affiliation: Genetisches Institut der Universität München, Maria-Ward-Str. 1a, D-8000, München 19, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Grosch
authors.forename: C
authors.initials: C
authors.lastname: Schmelzer
authors.forename: S
authors.initials: S
authors.lastname: Mathews