title: A cellulosic responsive "living" membrane.
abstract: Bacterial cellulose has been demonstrated to be a remarkably versatile biomaterial and widely used in biomedical applications due to its unique physical properties. Here we reported for the first time a "living membrane" system based on recombinant Escherichia coli bacterial strains entrapped in cellulosic membranes produced by Gluconacetobacter xylinus. Biologically driven detection and identification of a range of target molecules presents unique challenges, and requires that detection methods are developed to be rapid, specific and sensitive. The compatibility of G. xylinus and recombinant E. coli strains was first investigated for co-cultivation, and the relationship between the number of entrapped E. coli and the level of inducible signal achieved was further explored by fluorescent signal observation in confocal microscopy. Finally to amplify the response to inducers for maximum fluorescent signal, a positive-feedback genetic amplifier was designed within recombinant E. coli strain entrapped in the living cellulosic membrane system, allowing for the detection mechanism to be extremely sensitive and resulting in a significant fluorescent signal from a single receptor binding event. The living membrane system proposed here will create devices of greater complexity in function for applications in biological and chemical detection.
authors.affiliation: Department of Biomedical Engineering, 4 Colby Street, Tufts University, Medford, MA 02155, USA.
authors.forename: Guokui
authors.initials: G
authors.lastname: Qin
authors.forename: Bruce J
authors.initials: BJ
authors.lastname: Panilaitis
authors.forename: Zhongyuan Sun David L
authors.initials: ZS
authors.lastname: Kaplan
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D005246:Feedback; D005818:Genetic Engineering / Q000379:methods*; D041822:Gluconacetobacter xylinus / Q000166:cytology; D053038:Quorum Sensing; D012097:Repressor Proteins / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D012097:Repressor Proteins; D015534:Trans-Activators; C064778:LuxR autoinducer binding proteins; D002482:Cellulose
keywords: Bacterial cellulose; Co-cultivation; Gene amplifier; Living membrane; Positive feedback