title: Transformation of grape (Vitis vinifera L.) zygotic-derived somatic embryos and regeneration of transgenic plants.
abstract: Transgenic grape plants were regenerated from somatic embryos derived from immature zygotic embryos of seedless grape (Vitis vinifera L.) selections. Somatic embryos were bombarded twice with 1 μm gold particles using the Biolistic PDS-1000/He device (Bio-Rad Laboratories) and then exposed to Agrobacterium tumefaciens strain C58/Z707 containing the binary plasmid pGA482GG or pCGN7314. Following cocultivation, secondary embryos were allowed to proliferate on Emershad/Ramming proliferation (ERP) medium for 6 weeks before selection on ERP medium containing 20-40 μg/ml kanamycin (kan). Transgenic embryos were identified after 3-5 months under selection and allowed to germinate and develop into rooted plants on Woody Plant Medium containing 1 μM 6-benzylaminopurine (BAP), 1.5% sucrose, 0.3% activated charcoal and 0.75% agar. Integration of the foreign genes into these grapevines was verified by growth in the presence of kan, positive GUS and PCR assays, and Southern analysis.
authors.affiliation: USDA-ARS Appalachian Fruit Research Station, 45 Wiltshire Rd., 25430, Kearneysville, WV, USA.
authors.forename: R
authors.initials: R
authors.lastname: Scorza
authors.forename: J M
authors.initials: JM
authors.lastname: Cordts
authors.forename: D W
authors.initials: DW
authors.lastname: Ramming
authors.forename: R L
authors.initials: RL
authors.lastname: Emershad