title: [Advances in studying microbial GH13 starch debranching enzyme--a review].
abstract: Pullulanase and isoamylase belong to the GH13 family (glycoside hydrolase family 13) with similar sequence, catalytic mechanism and three-dimensional fold ((beta/alpha)8-barrel structure). Starch debranching enzymes can hydrolyze the alpha-1,6-glucosidic bonds at the branch sites of starch, and improve raw material utilization and production efficiency in the starch industry. In this review, the substrate specificity, protein structure, advances and new trends in the study of microbial GH13 starch debranching enzyme were systematically introduced. In addition, some opinions on the research status and prospect for starch debranching enzyme were discussed.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China. dw5601@163.com
authors.forename: Xuguo
authors.initials: X
authors.lastname: Duan
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
mesh_terms: D001419:Bacteria / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007517:Isoamylase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D013213:Starch / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D013213:Starch; D006026:Glycoside Hydrolases; C100181:pullulanase; D007517:Isoamylase