title: Sphingobacterium ginsenosidimutans sp. nov., a bacterium with ginsenoside-converting activity isolated from the soil of a ginseng field.
abstract: A Gram-staining-negative, aerobic, non-motile, non-spore-forming, and rod-shaped bacterium designated THG 07(T) was isolated from the soil of a ginseng field of Pocheon in South Korea, and its taxonomic position was investigated by using a polyphasic study. Strain THG 07(T) grew optimally at 25-30°C and at pH 6.5-7.0 and in the absence of NaCl on nutrient agar. Strain THG-T17(T) displayed β-glucosidase activity that was responsible for its ability to transform ginsenoside Rb1 (one of the dominant ginsenosides of ginseng) to compound C-K. On the basis of 16S rRNA gene sequence similarity, strain THG 07(T) was shown to belong to the family Sphingobacteriaceae and was related to Sphingobacterium canadense CR11(T) (98.7%), S. cladoniae No.6(T) (98.1%), S. detergens 6.2S(T) (98.0%), S. multivorum IAM14316(T) (97.9%), S. siyangense SY1(T) (97.8%) and S. thalpophilum DSM11723(T) (96.9%). The G+C content of the genomic DNA was 40.6 mol%. The major menaquinone, MK-7, and major fatty acids, iso-C₁₅:₀ and C₁₆:₁ ω7c and/or ω6c, supported the affiliation of strain THG 07(T) to the genus Sphingobacterium. The DNA-DNA relatedness values between strain THG 07(T) and its closest phylogenetic neighbors were below 26.9%. The results of physiological and biochemical tests enabled strain THG 07(T) to be differentiated phenotypically from the recognized species of the genus Sphingobacterium. Therefore the isolate represents a novel species, for which the name Sphingobacterium ginsenosidimutans sp. nov. is proposed, with the type strain THG 07(T) (=KACC 14526(T)=JCM 16722(T)).
authors.affiliation: Department of Oriental Medicinal Material & Processing College of Life Sciences, Kyung Hee University.
authors.forename: Heung-Min
authors.initials: HM
authors.lastname: Son
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Yang
authors.forename: Moo-Chang
authors.initials: MC
authors.lastname: Kook
authors.forename: Heon-Sub
authors.initials: HS
authors.lastname: Shin
authors.forename: Sang-Yong
authors.initials: SY
authors.lastname: Park
authors.forename: Don-Gill
authors.initials: DG
authors.lastname: Lee
authors.forename: Tae-Hoo
authors.initials: TH
authors.lastname: Yi
mesh_terms: D000332:Aerobiosis; D001482:Base Composition; D001711:Biotransformation; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D036145:Ginsenosides / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008969:Molecular Sequence Data; D005894:Panax / Q000254:growth & development; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D045249:Sphingobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D013696:Temperature; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D036145:Ginsenosides; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D001617:beta-Glucosidase