title: The biological activity of different forms of Polyoma Virus DNA and viral DNA fragments.
abstract: Mouse tissue culture cells were infected with different forms of Polyoma Virus (PV) DNA or virus DNA fragments by means of a microinjection technique and stained for PV-tumor (T) antigen and virus capsid (V) antigen 48 hr after injection.The efficiency of PV-DNA 1 (20S) to induce T- and V-antigen was within the same range as the efficiency of the full virus particle. DNA II (16S) showed a reduced capacity for both T- and V-antigen induction.Single stranded DNA molecules (16 or 18S) and double stranded DNA fragments (12S) led to T-antigen but not V-antigen synthesis. Simultaneous transfer of 16S and 18S DNA revealed T- and V-antigen formation.
authors.affiliation: Institut für Molekularbiologie und Biochemie der Freien Universität Berlin, Berlin_W., F.R.G..
authors.forename: M
authors.initials: M
authors.lastname: Graessmann
authors.forename: A
authors.initials: A
authors.lastname: Graessmann
authors.forename: E
authors.initials: E
authors.lastname: Hoffmann
authors.forename: J
authors.initials: J
authors.lastname: Niebel
authors.forename: K
authors.initials: K
authors.lastname: Pilaski