title: Diagnosis of a T-lineage acute lymphoblastic leukemia through digitalized cell analysis of the pleural effusion.
abstract: BACKGROUND
  Pleural effusion as the first clinical manifestation of acute lymphoblastic leukemia (ALL) is a relatively rare event. An early and accurate diagnosis of this clinical picture is very important for adequate patient management.
  
  
  METHODS
  We report the atypical onset of T-lineage ALL in a 31-year-old man. The patient was admitted to the emergency room due to lung failure; at that moment, the patient's initial blood count was normal; the chest X-ray radiography showed a massive pleural effusion and a thoracentesis was carried out. Routine investigations performed on the pleural fluid using a new technology system and digitalized cell analysis demonstrated infiltration by immature cells. Therefore, bone marrow aspirate and flow cytometry analyses were performed, leading to the diagnosis of T-lineage ALL. A cord blood transplantation procedure was performed at the first hematological remission following chemotherapy regimens. The patient died of septic shock.
  
  
  CONCLUSIONS
  The case we reported underlines the usefulness of using automated instruments to identify abnormal lymphoid cells in body fluids.
authors.affiliation: General Laboratory Unit (Microscopy and Clinical Cytometry Unit), Firenze, Italy.
authors.forename: Benedetta
authors.initials: B
authors.lastname: Peruzzi
authors.forename: Ilaria
authors.initials: I
authors.lastname: Cutini
authors.forename: Anna Maria Grazia
authors.initials: AM
authors.lastname: Gelli
authors.forename: Tommaso
authors.initials: T
authors.lastname: Rondelli
authors.forename: Marinella
authors.initials: M
authors.lastname: Statello
authors.forename: Sara
authors.initials: S
authors.lastname: Bencini
authors.forename: Francesco
authors.initials: F
authors.lastname: Mannelli
authors.forename: Roberto
authors.initials: R
authors.lastname: Caporale
authors.forename: Alberto
authors.initials: A
authors.lastname: Bosi
authors.forename: Alessandra
authors.initials: A
authors.lastname: Fanelli
keywords: digital morphology; leukemia; pleural effusion