title: Expression of an abscisic acid responsive promoter in Picea abies (L.) Karst. following bombardment from an electric discharge particle accelerator.
abstract: The 1.5 kilobase promoter sequence upstream of Dc8, a late embryo abundant gene of Daucus, fused to the reporter β-glucuronidase gene was introduced into several tissues of Picea abies via a custom-made electric-discharge particle accelerator. Transient expression was measured histochemically as spot number 2 d after bombardment. Embryogenic suspensions gave higher levels of expression depending upon cell line than embryogenic callus or zygotic embryos. Expression was enhanced when cultures were treated with abscisic acid for 3 d before bombardment. A mean and maximum of 17 and 34 spots/disk, respectively, were observed with the best cell line, which was comparable with the level of expression driven by an enhanced 35S promoter.
authors.affiliation: Department of Forest Science, Texas Agricultural Experiment Station, Texas A & M University System, 77843-2135, College Station, TX.
authors.forename: R J
authors.initials: RJ
authors.lastname: Newton
authors.forename: H S
authors.initials: HS
authors.lastname: Yibrah
authors.forename: N
authors.initials: N
authors.lastname: Dong
authors.forename: D H
authors.initials: DH
authors.lastname: Clapham
authors.forename: S
authors.initials: S
authors.lastname: von Arnold