title: [Detection of FasL mRNA, sFasL and their regulatory effect on T lymphocyte subsets in patients with severe acute pancreatitis].
abstract: OBJECTIVE
  To investigate the levels of FasL mRNA in peripheral blood mononualear cells (PBMCs), serum soluble Fas ligand (sFasL) and their regulatory effect on T lymphocyte subsets in patients with severe acute pancreatitis (SAP).
  
  
  METHODS
  Forty-eight patients with pancreatitis were randomly divided into two groups: 20 cases with SAP and 28 cases with mild acute pancreatitis (MAP). Twenty-eight healthy volunteers were selected as control group. The expression of FasL mRNA in PBMCs was detected by real-time quantitative PCR(qRT-PCR), and serum sFasL was measured by ELISA. T lymphocyte subsets in peripheral blood were detected by flow cytometry.
  
  
  RESULTS
  Compared with control group and MAP group, FasL mRNA of PBMCs and serum sFasL increased significantly in SAP group (P<0.05), a little increase in MAP group, and there was no significant difference between MAP group and control group (P>0.05). The CD4(+) T cell ratio, CD4(+)/CD8(+) ratio decreased significantly in SAP group (P<0.05) vs control group and MAP group), and they were found negatively related to FasL mRNA, serum sFasL level.
  
  
  CONCLUSIONS
  The SAP patients showed the significantly increased FasL mRNA of PBMCs and serum sFasL and decreased CD4(+) T-cell ratio, CD4(+)/CD8(+) ratio. FasL may mediate the apoptosis of T lymphocytes.
authors.affiliation: Department of Digestive Medicine, Affiliated Hospital, Youjiang Medical College for Nationalities, Baise 533000, China.
authors.forename: Yueqiu
authors.initials: Y
authors.lastname: Qin
authors.forename: Pinhu
authors.initials: P
authors.lastname: Liao
authors.forename: Shougao
authors.initials: S
authors.lastname: He
authors.forename: Yixia
authors.initials: Y
authors.lastname: Yin
authors.forename: Sien
authors.initials: S
authors.lastname: Song
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.forename: Pin
authors.initials: P
authors.lastname: Zheng
mesh_terms: D000208:Acute Disease; D000328:Adult; D016022:Case-Control Studies; D053222:Fas Ligand Protein / Q000097:blood* / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D010195:Pancreatitis / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012995:Solubility; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism*
chemical_list: D053222:Fas Ligand Protein; D012333:RNA, Messenger