title: Validation of the QIAsymphony RGQ system for DNA quantitation of different BK virus genotypes in whole blood samples.
abstract: Human polyomavirus BK (BKV) is increasingly recognized as an opportunistic pathogen in transplant recipients. The aim of this work was to evaluate the artus(®) BK Virus QS-RGQ assay on the QIAsymphony RGQ system in whole blood (WB) samples (tests performed in an off-label capacity) according to different BKV genotypes by comparison with a laboratory-developed assay. BKV loads were measured in 111 WB samples and BKV genotype was determined by sequencing the full-length VP1 gene. The artus(®) assay exhibited a limit of detection of 77copies/mL, a linearity range from 3.0 to 6.0log10copies/mL, intra-assay and inter-assay coefficients of variation ranging from 0.65% to 5.18%. Regarding BKV quantitation, artus(®) and laboratory-developed assays were highly correlated (Spearman correlation coefficient Rho=0.79; P<0.0001) with an excellent overall agreement (96.4%) and no significant quantitative difference according to Bland-Altman analysis (mean difference: -0.34log10copies/mL). The results did not show any influence of BKV genotype on BKV quantitation by the artus(®) assay, except a potential underquantitation of BKV subtype Ia which deserves further confirmation. In conclusion, the QIAsymphony RGQ system appears to be appropriate for the quantitation of BKV load in WB samples.
authors.affiliation: UPMC Univ Paris 06, ER1 DETIV, Paris, France; Service de Virologie, Hôpitaux Universitaires La Pitié Salpêtrière - Charles Foix, AP-HP, Paris, France.
authors.forename: Sonia
authors.initials: S
authors.lastname: Burrel
authors.affiliation: Service de Virologie, Hôpitaux Universitaires La Pitié Salpêtrière - Charles Foix, AP-HP, Paris, France.
authors.forename: Christel
authors.initials: C
authors.lastname: Brunet
authors.affiliation: Service de Virologie, Hôpitaux Universitaires La Pitié Salpêtrière - Charles Foix, AP-HP, Paris, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Hamm
authors.affiliation: UPMC Univ Paris 06, ER1 DETIV, Paris, France; Service de Virologie, Hôpitaux Universitaires La Pitié Salpêtrière - Charles Foix, AP-HP, Paris, France.
authors.forename: Maud
authors.initials: M
authors.lastname: Gits-Muselli
authors.affiliation: Service de Virologie, Hôpitaux Universitaires La Pitié Salpêtrière - Charles Foix, AP-HP, Paris, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Hermet
authors.affiliation: Service de Virologie, Hôpitaux Universitaires La Pitié Salpêtrière - Charles Foix, AP-HP, Paris, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Aimé
authors.affiliation: UPMC Univ Paris 06, ER1 DETIV, Paris, France; Service de Virologie, Hôpitaux Universitaires La Pitié Salpêtrière - Charles Foix, AP-HP, Paris, France.
authors.forename: Henri
authors.initials: H
authors.lastname: Agut
authors.affiliation: UPMC Univ Paris 06, ER1 DETIV, Paris, France; Service de Virologie, Hôpitaux Universitaires La Pitié Salpêtrière - Charles Foix, AP-HP, Paris, France. Electronic address: david.boutolleau@psl.aphp.fr.
authors.forename: David
authors.initials: D
authors.lastname: Boutolleau
mesh_terms: D001739:BK Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001769:Blood / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics*; D005838:Genotype; D006801:Humans; D027601:Polyomavirus Infections / Q000821:virology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014412:Tumor Virus Infections / Q000821:virology*; D019562:Viral Load / Q000379:methods*
chemical_list: D004279:DNA, Viral
keywords: Automation; BK virus; Genotype; Viral load; Whole blood