title: Agronomic evaluation of tissue-culture-derived soybean plants.
abstract: Genetic alterations of regenerated plants based on the tissue culture process (somaclonal variation) have become common for many plant species including soybean [Glycine max (L.) Merr.]. The objective of this study was to test for the presence of tissue-culture-derived genetic variation in eight agronomic traits in homozygous progeny regenerated by organogenesis using the commercially important cultivar Asgrow 'A3127.' A total of 86 lines derived by repeated self-pollination of nine regenerated plants was grown in two locations for 2 years. When compared to the unregenerated parent, statistically significant variation (P<0.05) was found for maturity, lodging, height, seed protein and oil, but not for seed quality, seed weight, or seed yield. All of the variation noted was beneficial and did not involve decreased yield. Since the differences were not large, the results indicate that the tissue culture process is not necessarily detrimental to plant performance, which is an important consideration since tissue culture techniques are used in many genetic engineering methods.
authors.affiliation: Department of Agronomy, University of Illinois, 61801, Urbana, IL, USA.
authors.forename: P A
authors.initials: PA
authors.lastname: Stephens
authors.forename: C D
authors.initials: CD
authors.lastname: Nickell
authors.forename: J M
authors.initials: JM
authors.lastname: Widholm