title: Curcumin attenuates oxidative stress following downhill running-induced muscle damage.
abstract: Downhill running causes muscle damage, and induces oxidative stress and inflammatory reaction. Recently, it is shown that curcumin possesses anti-oxidant and anti-inflammatory potentials. Interestingly, curcumin reduces inflammatory cytokine concentrations in skeletal muscle after downhill running of mice. However, it is not known whether curcumin affects oxidative stress after downhill running-induced muscle damage. Therefore, the purpose of this study was to investigate the effects of curcumin on oxidative stress following downhill running induced-muscle damage. We also investigated whether curcumin affects macrophage infiltration via chemokines such as MCP-1 and CXCL14. Male C57BL/6 mice were divided into four groups; rest, rest plus curcumin, downhill running, or downhill running plus curcumin. Downhill running mice ran at 22 m/min, -15% grade on the treadmill for 150 min. Curcumin (3mg) was administered in oral administration immediately after downhill running. Hydrogen peroxide concentration and NADPH-oxidase mRNA expression in the downhill running mice were significantly higher than those in the rest mice, but these variables were significantly attenuated by curcumin administration in downhill running mice. In addition, mRNA expression levels of MCP-1, CXCL14 and F4/80 reflecting presence of macrophages in the downhill running mice were significantly higher than those in the rest mice. However, MCP-1 and F4/80 mRNA expression levels were significantly attenuated by curcumin administration in downhill running mice. Curcumin may attenuate oxidative stress following downhill running-induced muscle damage.
authors.affiliation: Graduate School of Sport Sciences, Waseda University, Tokorozawa, Saitama, Japan. Electronic address: kawanishinoriaki@akane.waseda.jp.
authors.forename: Noriaki
authors.initials: N
authors.lastname: Kawanishi
authors.forename: Kouki
authors.initials: K
authors.lastname: Kato
authors.forename: Masaki
authors.initials: M
authors.lastname: Takahashi
authors.forename: Tsubasa
authors.initials: T
authors.lastname: Mizokami
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Otsuka
authors.forename: Atsushi
authors.initials: A
authors.lastname: Imaizumi
authors.forename: Daisuke
authors.initials: D
authors.lastname: Shiva
authors.forename: Hiromi
authors.initials: H
authors.lastname: Yano
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage*; D000943:Antigens, Differentiation / Q000096:biosynthesis; D018932:Chemokine CCL2 / Q000096:biosynthesis; D019743:Chemokines, CXC / Q000096:biosynthesis; D003474:Curcumin / Q000008:administration & dosage*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000293:injuries* / Q000378:metabolism; D019255:NADPH Oxidases / Q000096:biosynthesis; D018384:Oxidative Stress / Q000187:drug effects*; D012333:RNA, Messenger / Q000096:biosynthesis; D012420:Running / Q000293:injuries*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000943:Antigens, Differentiation; C517518:CXCL14 protein, mouse; C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D019743:Chemokines, CXC; D012333:RNA, Messenger; C036763:monocyte-macrophage differentiation antigen; D006861:Hydrogen Peroxide; D019255:NADPH Oxidases; D003474:Curcumin
keywords: Curcumin; Downhill running; Macrophage; Oxidative stress; Skeletal muscle