title: Cellular fractionation--mammalian cells.
abstract: In proteomics research, one essential step among enrichment techniques is subcellular fractionation. This is of special importance for analyzing intracellular organelles and multiprotein complexes. Subcellular fractionation is a flexible and adjustable approach to reducing sample complexity and is most efficiently combined with high-resolution 2-D gel/mass spectrometry analysis as well as with gel-independent techniques.
authors.affiliation: Merck KGaA, Darmstadt, Germany. Electronic address: Joerg.von.Hagen@merck.de.
authors.forename: Joerg
authors.initials: J
authors.lastname: von Hagen
authors.forename: Uwe
authors.initials: U
authors.lastname: Michelsen
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000295:instrumentation / Q000379:methods*; D008322:Mammals; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D013347:Subcellular Fractions
keywords: Cellular fractionation; Determination of marker enzyme; Identification of proteins; Mammalian cells; Protein translocation; Subcellular mammalian cell fractionation