title: Effect of recombinant human interleukin 1β (rhIL-1β) on amino acid flux in the isolated perfused rat liver.
abstract: We studied the effect of recombinant human IL-1β (rhIL-1β) on hepatic amino acid (AA) flux in the isolated perfused rat liver model. Two experimental groups were used - a control group (n = 5) and a rhIL-1β-treated group (n = 5). IL-1 was added to the perfusate in two successive boluses of 0.1µg and 0.9µg, respectively 35 min (final concentration 0.67 ng/ml) and 60 min (6 ng/ml) after beginning the perfusion. In the IL-1 treated group, a reduction in flux was observed for only three AA, alanine, phenylalanine and serine. Glucose and urea production in the IL-1-treated group was slightly but not-significantly lower than in the controls.rhIL-1β thus has only minor direct effects on AA flux and gluconeogenesis in the liver and cannot therefore be held responsible for the increase in hepatic amino acid uptake during stress.
authors.affiliation: Laboratoire de Biochimie A, Hôpital Saint Antoine, 184, rue du Fbg Saint Antoine, F-75571, Paris Cedex 12, France.
authors.forename: S K
authors.initials: SK
authors.lastname: Lim
authors.forename: J P
authors.initials: JP
authors.lastname: De Bandt
authors.forename: F
authors.initials: F
authors.lastname: Ballet
authors.forename: C
authors.initials: C
authors.lastname: Rey
authors.forename: C
authors.initials: C
authors.lastname: Coudray-Lucas
authors.forename: F
authors.initials: F
authors.lastname: Blonde-Cynober
authors.forename: J
authors.initials: J
authors.lastname: Giboudeau
authors.forename: L
authors.initials: L
authors.lastname: Cynober