title: Plant regeneration from protoplasts of peppermint (Mentha piperita L.).
abstract: Enzymatically isolated leaf-derived protoplasts of peppermint (Mentha piperita L.) were cultured in modified B5 medium containing 1 mg/l NAA, 0.4 mg/l BA, 0.5% sucrose, 0.5 M mannitol and 0.1% Gelrite (first medium). After 30 d culture at 25°C in the dark, protoplasts formed colonies consisting of about 100 cells. Gelrite medium blocks were transferred into liquid medium to promote further growth. Colonies of 0.5 mm transferred to 0.2% Gelrite solidified medium (same components as first medium) formed green calli (1-2 mm) under incubation in the light. Green calli transferred to differentiation medium (B5, 0.1 mg/l NAA, 5 mg/l BA, 2% sucrose, 0.2 M mannitol, 0.2% Gelrite) developed shoot buds after 3-4 weeks. Whole plants were recovered following rooting of shoots in B5 medium without hormones.
authors.affiliation: Lotte Central Laboratory Co., Ltd., 3-1-1, Numakage, Urawa, 336, Saitama, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Sato
authors.forename: S
authors.initials: S
authors.lastname: Enomoto
authors.forename: S
authors.initials: S
authors.lastname: Oka
authors.forename: K
authors.initials: K
authors.lastname: Hosomi
authors.forename: Y
authors.initials: Y
authors.lastname: Ito