title: A reusable and sensitive biosensor for total mercury in canned fish based on fluorescence polarization.
abstract: In this work, we developed a sensitive and selective sensor technique for total mercury (Hg) detection in canned fish samples based on the fluorescence polarization (FP) method. The detection principle was that ssDNA containing thymine (T) bases was modified on magnetic nanoparticles (MNPs), which were used as enhancement probe. In the presence of Hg(2+), the ssDNA on MNPs can hybridize with the fluorophore labeled aptamer owing to the specific interaction between T bases and Hg(2+). The formation of thymine-Hg(2+)-thymine (T-Hg(2+)-T) complexes leads to the molar mass increase of fluorophore molecules, resulting in the enhancement of FP signal. The increase of FP was in a good linearity with the concentration of Hg(2+) in range of 2.0 nM-1.0 mM and the limit of detection was 0.49 nM (3.29 SB/m, according to the recent recommendation of IUPAC). Moreover, the proposed biosensor can be reused for 6 cycling times and was successfully applied in monitoring Hg(2+) in real samples.
authors.affiliation: Department of Chemistry, Liaocheng University, Liaocheng 252059, PR China.
authors.forename: Tongfei
authors.initials: T
authors.lastname: Shen
authors.forename: Qiaoli
authors.initials: Q
authors.lastname: Yue
authors.forename: Xiuxiu
authors.initials: X
authors.lastname: Jiang
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Shuling
authors.initials: S
authors.lastname: Xu
authors.forename: Haibo
authors.initials: H
authors.lastname: Li
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Gu
authors.forename: Shuqiu
authors.initials: S
authors.lastname: Zhang
authors.forename: Jifeng
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods* / Q000592:standards; D004277:DNA, Single-Stranded / Q000737:chemistry; D017743:Equipment Reuse; D005399:Fishes / Q000378:metabolism; D005452:Fluoresceins; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D005506:Food Contamination / Q000032:analysis*; D057141:Food, Preserved / Q000032:analysis*; D006801:Humans; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry; D008628:Mercury / Q000032:analysis*; D013941:Thymine / Q000737:chemistry
chemical_list: D004277:DNA, Single-Stranded; D005452:Fluoresceins; D005456:Fluorescent Dyes; D058185:Magnetite Nanoparticles; C024098:6-carboxyfluorescein; D008628:Mercury; D013941:Thymine
keywords: Canned fish; Fluorescence Polarization; Magnetic nanoparticles; Mercury