title: Removal of toxic Co-EDTA complex by a halophilic solar-salt-pan isolate Pseudomonas aeruginosa SPB-1.
abstract: In this study, a promising bioremediation approach was developed to remove [Co(III)-EDTA](-) complex that is generated during the waste management process. Though several studies have been reported on bioremediation of cobalt, the removal of [Co(III)-EDTA](-) complex has not been tested. A [Co(III)-EDTA](-) resistant bacterium, Pseudomonas aeruginosa SPB-1 was isolated from the solar-salt-pan and physical parameters were optimized for its growth. The various studies showed that the removal of [Co(III)-EDTA](-) from the bulk liquid was due to the adsorption of the complex by the biomass. Using absorption/desorption isotherm over a range of pH (1-8), the maximum adsorption of [Co(III)-EDTA](-) was found to be at pH 7.0 and maximum desorption from the biomass occurred at pH 1.0, thus rendering an ion exchange property to P. aeruginosa SPB-1 biomass. P. aeruginosa SPB-1 biomass could be used as bio-resin that showed 80.4±3.27% adsorption capacity up to fourth cycle and the biomass was viable till the ninth cycle with 10.5±7.3% adsorption. Radiation tolerance potential i.e. D10 value for the strain was found to be ~300 Gy, which suggests the potential use of the bacterium in bioremediation of moderately active nuclear waste.
authors.affiliation: Departartment of Biotechnology, Pondicherry University, Puducherry, India.
authors.forename: A
authors.initials: A
authors.lastname: Paraneeiswaran
authors.forename: Sudhir K
authors.initials: SK
authors.lastname: Shukla
authors.forename: T
authors.initials: T
authors.lastname: Subba Rao
authors.forename: K
authors.initials: K
authors.lastname: Prashanth
mesh_terms: D000327:Adsorption; D001673:Biodegradation, Environmental; D018533:Biomass; D003035:Cobalt / Q000378:metabolism* / Q000633:toxicity; D056831:Coordination Complexes / Q000378:metabolism* / Q000633:toxicity; D004492:Edetic Acid / Q000378:metabolism* / Q000633:toxicity; D006863:Hydrogen-Ion Concentration; D011550:Pseudomonas aeruginosa / Q000502:physiology*
chemical_list: D056831:Coordination Complexes; D003035:Cobalt; D004492:Edetic Acid
keywords: Bioremediation; Biosorption; Cobalt; Pseudomonas aeruginosa; [Co(III)-EDTA](−)