title: MRI reveals edema in larynx (but not in brain) during anaphylactic hypotension in anesthetized rats.
abstract: OBJECTIVE
  Anaphylactic shock is sometimes accompanied by local interstitial edema due to increased vascular permeability. We performed magnetic resonance imaging (MRI) to compare edema in the larynx and brain of anesthetized rats during anaphylactic hypotension versus vasodilator-induced hypotension.
  
  
  METHODS
  Male Sprague Dawley rats were subjected to hypotension induced by the ovalbumin antigen (n=7) or a vasodilator sodium nitroprusside (SNP; n=7). Apparent diffusion coefficient (ADC) and T2-relaxation time (T2RT) were quantified on MRI performed repeatedly for up to 68 min after the injection of either agent. The presence of laryngeal edema was also examined by histological examination. Separately, the occurrence of brain edema was assessed by measuring brain water content using the wet/dry method in rats with anaphylaxis (n=5) or SNP (n=5) and the non-hypotensive control rats (n=5). Mast cells in hypothalamus were morphologically examined.
  
  
  RESULTS
  Mean arterial blood pressure similarly decreased to 35 mmHg after an injection of the antigen or SNP. Hyperintensity on T2-weighted images (as reflected by elevated T2RT) was found in the larynx as early as 13 min after an injection of the antigen, but not SNP. A postmortem histological examination revealed epiglottic edema in the rats with anaphylaxis, but not SNP. In contrast, no significant changes in T2RT or ADC were detectable in the brains of any rats studied. In separate experiments, the quantified brain water content did not increase in either anaphylaxis or SNP rats, as compared with the non-hypotensive control rats. The numbers of mast cells with metachromatic granules in the hypothalamus were not different between rats with anaphylaxis and SNP, suggesting the absence of anaphylactic reaction in hypothalamus.
  
  
  CONCLUSIONS
  Edema was detected using the MRI technique in the larynx during rat anaphylaxis, but not in the brain.
authors.affiliation: Department of Radiology, Kanazawa Medical University, Uchinada, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Toyota
authors.forename: Mamoru
authors.initials: M
authors.lastname: Tanida
authors.forename: Toshishige
authors.initials: T
authors.lastname: Shibamoto
authors.forename: Mofei
authors.initials: M
authors.lastname: Wang
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Kurata
authors.forename: Hisao
authors.initials: H
authors.lastname: Tonami
keywords: Anaphylactic shock; brain edema; laryngeal edema; mast cell