title: A novel tomato MYC-type ICE1-like transcription factor, SlICE1a, confers cold, osmotic and salt tolerance in transgenic tobacco.
abstract: ICE1 (inducer of CBF expression 1), a MYC-type bHLH transcription factor, is an important activator of CBF3/DREB1A for regulating cold signaling and stress tolerance. In this study, we isolated the novel ICE1-like gene SlICE1a from tomato which contains the conserved bHLH domain, an S-rich motif, and ACT-domain. It is localized in the nucleus and harbors transcription-activating activity in the N-terminal. In addition, the SlICE1a transcript is slightly upregulated by cold stress, salt stress, and osmotic stress. SlICE1a overexpression in tobacco enhances the induction of CBF/DREB and their target genes, consequently increasing the levels of proline, soluble sugars, and late embryogenesis abundant (LEA) proteins, and enhancing tolerance to cold stress, osmotic stress, and salt stress. SlICE1a functions in abiotic stress responses by regulating the expression of stress-tolerant genes, and is thus beneficial for crop improvement.
authors.affiliation: College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Feng
authors.forename: Na-Na
authors.initials: NN
authors.lastname: Ma
authors.forename: Xia
authors.initials: X
authors.lastname: Meng
authors.forename: Song
authors.initials: S
authors.lastname: Zhang
authors.forename: Jie-Ru
authors.initials: JR
authors.lastname: Wang
authors.forename: Sen
authors.initials: S
authors.lastname: Chai
authors.forename: Qing-Wei
authors.initials: QW
authors.lastname: Meng
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism; D050260:Carbohydrate Metabolism; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism; D009997:Osmotic Pressure; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011392:Proline / Q000378:metabolism; D055049:Salt Tolerance / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D010940:Plant Proteins; D011392:Proline; D014157:Transcription Factors; C066246:late embryogenesis abundant protein, plant
keywords: 4′,6-diamidino-2-phenylindole; Abiotic stress; C-repeat binding factor; CBF; DAPI; DREB; Expression pattern; ICE; Inducer of CBF expression 1a; LEA; LT; MDA; MV; O(2)(−); PEG; PFD; ROS; TBA; TCA; Tomato; Transcription factor; Transgenic tobacco; WT; drought responsive element binding proteins; inducer of CBF expression; late embryogenesis abundant proteins; low temperature; malondialdehyde; methyl viologen; photon flux density; polyethylene glycol; reactive oxygen species; superoxide anion radical; thiobarbituric acid; trichloroacetic acid; wild-type