title: Acidobacteria from oligotrophic soil from the Cerrado can grow in a wide range of carbon source concentrations.
abstract: Soils from the Brazilian Cerrado are nutrient-poor, acidic, and aluminum-rich. A previous study revealed that members of the phylum Acidobacteria were predominant in these oligotrophic soils. Five acidobacteria from Cerrado soil were isolated on VL-55 medium containing 0.05% of xylan as carbon source. All isolates belong to the Acidobacteria subdivision 1, and their 16S rRNA showed similarities of 94.2%-96% with Acidobacterium capsulatum or 98.6% with Edaphobacter aggregans. All isolates were able to sustain growth in a wide range of carbon source concentrations. Growth occurred in all concentrations of arabinose, dextrose, and xylose; only one isolate did not grow on fructose. Isolates grew poorly on N-acetyl-D-glucosamine at all concentrations tested. In general, increasing concentrations of these monosaccharides did not inhibit growth rates. Isolates exhibited growth on solid medium containing xylan, carboxymethyl cellulose, and colloidal chitin; however, growth was observed on solid medium that did not contain these polysaccharides. These isolates may be able to use the solidifying agents tested (gellan gum or agar) as carbon source. This interpretation is supported by the absence of growth in liquid media containing chitin or carboxymethyl cellulose at 0.05% as sole carbon source, whereas growth in the same conditions using xylan was confirmed.
authors.affiliation: a Universidade Católica de Brasília, Graduate Program in Genomic Sciences and Biotechnology, SGAN 916, Brasília - DF 70790-160, Brazil.
authors.forename: Virgilio Hipólito Lemos
authors.initials: VH
authors.lastname: de Castro
authors.forename: Luis Felipe
authors.initials: LF
authors.lastname: Schroeder
authors.forename: Betania Ferraz
authors.initials: BF
authors.lastname: Quirino
authors.forename: Ricardo Henrique
authors.initials: RH
authors.lastname: Kruger
authors.forename: Cristine Chaves
authors.initials: CC
authors.lastname: Barreto
mesh_terms: D000117:Acetylglucosamine / Q000235:genetics / Q000378:metabolism; D061271:Acidobacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D001938:Brazil; D002244:Carbon / Q000378:metabolism*; D003470:Culture Media; D004269:DNA, Bacterial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000235:genetics; D009005:Monosaccharides / Q000378:metabolism; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009005:Monosaccharides; D012336:RNA, Ribosomal, 16S; D012987:Soil; D002244:Carbon; D000117:Acetylglucosamine