title: A mechanism for selective lymphocyte homing in bovine hemal nodes.
abstract: The distribution and abundance of MECA-79(+) cells in bovine hemal nodes (HNs) was analyzed. In addition, T cell subsets which expressed the homing receptor l-selectin were analyzed by flow cytometry. The frequency of MECA-79(+) HNs varied depending on the location. There were 61, 33, and 17% MECA-79(+) HN in the subcutaneous region, along the abdominal aorta and in the mesenteric region, respectively. MECA-79(+) cells in mesenteric lymph nodes were mainly distributed in the paracortex. Many MECA-79(+) cells were distributed in the perifollicular area of HNs and a few positive cells were also located in the paracortical and interfollicular areas. CD4(+) and CD8(+)l-selectin(+) cells were significantly higher in MECA-79(+) HNs compared to MECA-79(-) HNs. The data suggest that a selective lymphocyte homing mechanism exists in bovine HNs, which could be related to functional differences between locations.
authors.affiliation: Department of Veterinary Anatomy, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen Kibanadai-Nishi, Miyazaki 889-2192, Japan.
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhang
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nasu
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yasuda
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002417:Cattle / Q000276:immunology*; D019070:Cell Lineage; D002465:Cell Movement; D005260:Female; D008198:Lymph Nodes / Q000166:cytology*; D008214:Lymphocytes / Q000502:physiology*; D008297:Male; D008565:Membrane Proteins / Q000032:analysis
chemical_list: D000954:Antigens, Surface; C069886:L-selectin counter-receptors; D008565:Membrane Proteins
keywords: Bovine; Flow cytometry; Hemal node; Immunohistochemistry; MECA-79