title: Impact of partial pressure of oxygen in blood samples on the performance of systems for self-monitoring of blood glucose.
abstract: BACKGROUND
  The partial pressure of oxygen (pO2) in blood samples can affect glucose measurements with oxygen-sensitive systems. In this study, we assessed the influence of different pO2 levels on blood glucose (BG) measurements with five glucose oxidase (GOD) systems and one glucose dehydrogenase (GDH) system. All selected GOD systems were indicated by the manufacturers to be sensitive to increased oxygen content of the blood sample.
  
  
  METHODS
  Venous blood samples of 16 subjects (eight women, eight men; mean age, 52 years; three with type 1 diabetes, four with type 2 diabetes, and nine without diabetes) were collected. Aliquots of each sample were adjusted to the following pO2 values: ≤45 mm Hg, approximately 70 mm Hg, and ≥150 mm Hg. For each system, five consecutive measurements on each sample were performed using the same test strip lot. Relative differences between the mean BG value at a pO2 level of approximately 70 mm Hg, which was considered to be similar to pO2 values in capillary blood samples, and the mean BG value at pO2 levels ≤45 mm Hg and ≥150 mm Hg were calculated.
  
  
  RESULTS
  The GOD systems showed mean relative differences between 11.8% and 44.5% at pO2 values ≤45 mm Hg and between -14.6% and -21.2% at pO2 values ≥150 mm Hg. For the GDH system, the mean relative differences were -0.3% and -0.2% at pO2 values ≤45 mm Hg and ≥150 mm Hg, respectively.
  
  
  CONCLUSIONS
  The magnitude of the pO2 impact on BG measurements seems to vary among the tested oxygen-sensitive GOD systems. The pO2 range in which oxygen-sensitive systems operate well should be provided in the product information.
authors.affiliation: Institut für Diabetes-Technologie Forschungs- und Entwicklungsgesellschaft mbH an der Universität Ulm , Ulm, Germany .
authors.forename: Christina
authors.initials: C
authors.lastname: Schmid
authors.forename: Annette
authors.initials: A
authors.lastname: Baumstark
authors.forename: Stefan
authors.initials: S
authors.lastname: Pleus
authors.forename: Cornelia
authors.initials: C
authors.lastname: Haug
authors.forename: Martina
authors.initials: M
authors.lastname: Tesar
authors.forename: Guido
authors.initials: G
authors.lastname: Freckmann
mesh_terms: D015374:Biosensing Techniques; D001784:Blood Gas Analysis; D001786:Blood Glucose / Q000378:metabolism*; D015190:Blood Glucose Self-Monitoring / Q000295:instrumentation; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000503:physiopathology; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000503:physiopathology; D005260:Female; D042843:Glucose 1-Dehydrogenase / Q000097:blood*; D005949:Glucose Oxidase / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008991:Monitoring, Physiologic; D010100:Oxygen / Q000097:blood*; D010313:Partial Pressure; D015203:Reproducibility of Results
chemical_list: D001786:Blood Glucose; D042843:Glucose 1-Dehydrogenase; D005949:Glucose Oxidase; D010100:Oxygen