title: [Simultaneous determination of chlorogenic acid, scutellarin, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid in different parts of Erigeron breviscapus by high-performance liquid chromatography].
abstract: OBJECTIVE
  A high-performance liquid chromatographic (HPLC) method was developed for simultaneous determination of chlorogenic acid, scutellarin, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid in different parts of Erigerontis Herba.
  
  
  METHODS
  The four constituents were measured on an Agilent Zorbax SB-C18 column (4.6 mm x 450 mm, 5 microm) with a gradient elution of acetonitrile (A) -0.3% phosphoric acid solution (B) (0-10 min, 12%-15% A, 10-32 min, 15% A, 32-33 min, 15%-20% A, 33-50 min, 20%-22% A) at wavelength of 335 nm and 327 nm, and a flow rate of 1.0 mL x min(-1) and the column temperature was 30 degrees C.
  
  
  RESULTS
  Linearity of each standard was established in the concentration range of 0.050 1-1.002 microg for chlorogenic acid, 0.165 9-3.318 microg for chlorogenic acid, 0.049 7-0.994 microg for 3,5-dicaffeoylquinic acid, 0.048 7-0.974 p.g for 4,5-dicaffeoylquinic acid respectively, with correlation coefficient r > 0.999 6. Average recoveries (n = 6) of 4 compounds were 98.53% with a RSD of 0.94%, 99.68% with a RSD of 0.49%, 98.78% with a RSD of 1.1%, 99.06% with a RSD of 0.81%, respectively.
  
  
  CONCLUSIONS
  The developed method is simple, accurate, and precise, it can be used for the quantitative analysis of Erigeron breviscapus.
authors.affiliation: Yunnan Research Center on Good Agriculture Practice for Dominant Chinese Medicinal Meterials, Yunnan Agriculture University, Kunming 650201, China. lixiaobo-2007@163.com
authors.forename: Xiao-Bo
authors.initials: XB
authors.lastname: Li
authors.forename: Rui-Bo
authors.initials: RB
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Shen
authors.forename: Zhen-Gui
authors.initials: ZG
authors.lastname: Meng
authors.forename: Jun-Wen
authors.initials: JW
authors.lastname: Chen
authors.forename: Jian-Wen
authors.initials: JW
authors.lastname: Yang
authors.forename: Sheng-Chao
authors.initials: SC
authors.lastname: Yang
mesh_terms: D047310:Apigenin / Q000032:analysis* / Q000737:chemistry; D002726:Chlorogenic Acid / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D049919:Erigeron / Q000737:chemistry*; D005965:Glucuronates / Q000032:analysis* / Q000737:chemistry; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry
chemical_list: C480201:4,5-dicaffeoyl quinic acid; D004365:Drugs, Chinese Herbal; D005965:Glucuronates; D011801:Quinic Acid; C484876:scutellarin; D002726:Chlorogenic Acid; D047310:Apigenin; C100434:3,5-dicaffeoylquinic acid