title: A rapid and sensitive UPLC-MS/MS method for quantification of two caffeoylquinic acids and four main active components in rat plasma after an intravenous administration of Qingkailing injection and its application to a pharmacokinetic study.
abstract: Qingkailing (QKL) injection, a modified modern Chinese medicine preparation, is widely used in the clinic for its significant antipyretic and anti-inflammatory effects, but its serious adverse drug reactions have attracted more and more attention. Series of caffeoylquinic acids in QKL are widely suspected to be the allergens responsible for these adverse drug reactions. Therefore, pharmacokinetic studies of the caffeoylquinic acids are needed. In this paper, a simple, rapid and sensitive ultra-performance liquid chromatography-tandem mass spectrometry method was developed for the simultaneous determination of chlorogenic acid, neochlorogenic acid, baicalin, geniposide, cholic acid and hyodeoxycholic acid in rat plasma. Chromatographic separation was achieved on a BEH C18 column by a gradient elution at a flow rate of 0.40 mL/min in only 6.0 min. All analytes were monitored by multiple reaction monitoring mode with negative electrospray ionization. The calibration curves of these analytes were all linear (r > 0.9978) over wide concentration ranges. The intra- and inter- day precisions (relative standard deviations) were within 14.3% and accuracy (relative error) ranged from -6.8 to 4.8%. The mean recoveries ranged from 74.5 to 105.6%. This validated method was successfully applied to the pharmacokinetic study of the six analytes in rats following an intravenous administration of QKL injection.
authors.affiliation: Science Experiment Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, No. 11 North Third Ring Road, Chaoyang District, Beijing, 100029, People's Republic of China.
authors.forename: Long
authors.initials: L
authors.lastname: Peng
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Gao
authors.forename: Yuelin
authors.initials: Y
authors.lastname: Song
authors.forename: Longshan
authors.initials: L
authors.lastname: Zhao
authors.forename: Mingxing
authors.initials: M
authors.lastname: Guo
authors.forename: Jiankun
authors.initials: J
authors.lastname: Su
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Haiyu
authors.initials: H
authors.lastname: Liu
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008297:Male; D011801:Quinic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; C504928:Qingkailing; C472707:caffeoylquinic acid; D011801:Quinic Acid
keywords: Qingkailing injection; UPLC-MS/MS; caffeoylquinic acids; intravenous; pharmacokinetics