title: Combined anticalcification treatment of bovine pericardium with decellularization and hyaluronic acid derivative.
abstract: The objective of this work was to evaluate the effect of decellularization and hyaluronic acid derivative on the improvement of anticalcification of glutaraldehyde fixed bovine pericardium (GFBP) using a rat subcutaneous implantation model A cell extraction process was employed to remove the cells and cellular components from bovine pericardium (BP), leaving a framework of largely insoluble collagen. Then acellular BP was cross-linked by glutaraldehyde solution and treated with hyaluronic acid derivative (HA-ADH) which was obtained by coupling adipic dihydrazide (ADH) on-COOH of hyaluronic acid (HA). The results of in vivo calcification tests showed that the calcium content was decreased dramatically by decellularization alone (from 28.07 ± 18.87 to 2.44 ± 0.55 μg Ca/mg dry tissue after 8 weeks' implantation), and even less concentration was shown by the combination of HA derivative treatment and decellularization (GFaBP-HA group) (0.25 ± 0.08 μg Ca/mg dry tissue after 8 weeks' implantation). In addition, GFaBP-HA group not only presented a lower degree of calcification, but also showed lower ratios of Ca/P molar, which corresponded to amorphous calcium phosphates. The obtained results indicated that GFaBP-HA was a potential candidate for the manufacture of anticalcification bioprostheses.
authors.affiliation: School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology, 250353, Jinan, P.R. China.
authors.forename: Deyi
authors.initials: D
authors.lastname: Zhu
authors.forename: Liqiang
authors.initials: L
authors.lastname: Jin
authors.forename: Xuemei
authors.initials: X
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.forename: Tianqi
authors.initials: T
authors.lastname: Liu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001705:Bioprosthesis; D002114:Calcinosis / Q000503:physiopathology; D002118:Calcium / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry; D002417:Cattle; D003094:Collagen / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry*; D006834:Hydrazines / Q000737:chemistry; D008297:Male; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D010496:Pericardium / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D010758:Phosphorus / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013696:Temperature
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D003432:Cross-Linking Reagents; D006834:Hydrazines; D010758:Phosphorus; D006820:Hyaluronic Acid; D003094:Collagen; C020243:calcium phosphate; D002118:Calcium; D005976:Glutaral
keywords: Glutaraldehyde fixed bovine pericardium; anticalcification; decellularization; hyaluronic acid derivative