title: Changes in ATP, glucose-6-phosphate and NAD(P)H cellular levels during the proliferation and maturation phases of Abies alba Mill. embryogenic cultures.
abstract: The aim of the present study was to evaluate the adenosine triphospate (ATP), glucose-6-phosphate (glu-6P) and reduced form of nicotinamide adenine dinucleotide phosphate (NAD(P)H) cellular levels during the proliferation and maturation phases of Abies alba Mill. somatic embryos. For a better understanding of the dynamics of these parameters during the proliferation cycle, four embryonic cell lines were tested. During the maturation period, three independent experiments were conducted, focused on the effects of PEG-4000 (5 or 10% (w/v)) and abscisic acid (16, 32 or 64 μM) applied together (Experiments A and B) or with addition of gibberellic acid (Experiment C) on the dynamics of bio-energetic molecules and on the mean number of cotyledonary somatic embryos. Our results demonstrated that the cellular levels of bio-energetic molecules strongly depended on the composition of maturation media. Generally, the higher the number of cotyledonary embryos produced, the higher the level of ATP observed after a 2-week maturation period. The cellular level of ATP, glu-6P and NAD(P)H increased, particularly after the transition from the proliferation to the maturation phase when the differentiation and growth of somatic embryos occurred.
authors.affiliation: Faculty of Forestry and Wood Technology, Mendel University, Zemědělská 3, 613 00 Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Krajnáková
authors.forename: Alberto
authors.initials: A
authors.lastname: Bertolini
authors.forename: Laura
authors.initials: L
authors.lastname: Zoratti
authors.forename: Dusan
authors.initials: D
authors.lastname: Gömöry
authors.forename: Hely
authors.initials: H
authors.lastname: Häggman
authors.forename: Angelo
authors.initials: A
authors.lastname: Vianello
mesh_terms: D028202:Abies / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D000040:Abscisic Acid / Q000494:pharmacology; D000241:Adenosine / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism*; D018548:Cotyledon / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D018525:Germination / Q000187:drug effects; D005875:Gibberellins / Q000494:pharmacology; D019298:Glucose-6-Phosphate / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000494:pharmacology; D058439:Plant Somatic Embryogenesis Techniques; D011092:Polyethylene Glycols / Q000494:pharmacology; D012639:Seeds / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
chemical_list: D003470:Culture Media; D005875:Gibberellins; D010937:Plant Growth Regulators; D009243:NAD; D011092:Polyethylene Glycols; D009249:NADP; D019298:Glucose-6-Phosphate; D000040:Abscisic Acid; D000255:Adenosine Triphosphate; C007842:gibberellic acid; D000241:Adenosine
keywords: abscisic acid; bio-energetic molecules; gibberellic acid; maturation; polyethylene glycol; silver fir