title: Reconstitution of endoplasmic reticulum InsP3 receptors into black lipid membranes.
abstract: Intracellular ion channels, including endoplasmic reticulum (ER) calcium (Ca(2+)) channels, are most often studied through their reconstitution into planar lipid bilayers (also called black lipid membranes, or BLMs). General methods for making bilayers and for ion channel reconstitution into BLMs have been extensively detailed elsewhere; thus, here the focus is on specific details relevant for inositol(1,4,5)-trisphosphate receptor (InsP3R) recordings. These procedures describe how to perform single-channel recordings of native or recombinant InsP3Rs in BLMs. Similar procedures are used to study native or recombinant ryanodine receptors (RyanRs) in BLMs.
authors.affiliation: Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390;
authors.forename: Ilya
authors.initials: I
authors.lastname: Bezprozvanny
mesh_terms: D003584:Cytological Techniques / Q000379:methods; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000302:isolation & purification* / Q000378:metabolism*; D008051:Lipid Bilayers / Q000737:chemistry; D008566:Membranes / Q000737:chemistry* / Q000201:enzymology*
chemical_list: D053496:Inositol 1,4,5-Trisphosphate Receptors; D008051:Lipid Bilayers