title: Psychomotor seizure test, neurotoxicity and in vitro neuroprotection assay of some semicarbazone analogues.
abstract: In continuance of our search for anticonvulsant agents, we reported herein the synthesis, characterization and anticonvulsant evaluation of some newer semicarbazone analogues. A few compounds were also screened for neuroprotection assay. Some of the compounds showed significant anticonvulsant activity. Compound 4a showed 25% (1/4, 0.25 h), 75% (3/4, 0.5 & 2.0 h) and 100% (4/4, 1.0 h) protection against 6 Hz psychomotor seizure test at 100 mg/kg devoid of any neurotoxicity. Compound 4d showed neuroprotection activity with 26.3 ± 2.3 percent of total propidium iodide uptake at 100 μM and IC50 of the compound was calculated using dose response curve by probit analysis and was found to be 149 ± 1.22 μM.
authors.affiliation: Department of Pharmaceutical Chemistry, Maharishi Arvind College of Pharmacy, Jaipur, Rajasthan 302 023, India. jawedpharma@gmail.com.
authors.forename: Mohamed Jawed
authors.initials: MJ
authors.lastname: Ahsan
authors.forename: James P
authors.initials: JP
authors.lastname: Stables
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000138:chemical synthesis / Q000737:chemistry / Q000627:therapeutic use* / Q000633:toxicity; D016923:Cell Death; D004396:Coloring Agents / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D015195:Drug Design; D004353:Drug Evaluation, Preclinical; D004597:Electroshock; D017029:Epilepsy, Complex Partial / Q000188:drug therapy*; D006624:Hippocampus / Q000187:drug effects; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D020128:Inhibitory Concentration 50; D007608:Kainic Acid / Q000633:toxicity; D051379:Mice; D015394:Molecular Structure; D018696:Neuroprotective Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000627:therapeutic use* / Q000633:toxicity; D010433:Pentylenetetrazole / Q000633:toxicity; D011419:Propidium / Q000032:analysis; D012664:Semicarbazones / Q000138:chemical synthesis / Q000737:chemistry / Q000627:therapeutic use* / Q000633:toxicity
chemical_list: D000927:Anticonvulsants; D004396:Coloring Agents; D018696:Neuroprotective Agents; D012664:Semicarbazones; D011419:Propidium; D007608:Kainic Acid; D010433:Pentylenetetrazole