title: Inhibitors of ATP release inhibit vesicular nucleotide transporter.
abstract: Vesicular nucleotide transporter (VNUT) is responsible for vesicular ATP storage in ATP-secreting cells. In the present study, we examined the effects on VNUT-mediated transport of ATP release inhibitors such as ATP-binding cassette (ABC) proteins, hemichannels, maxi anion channels and P2X7 receptor. The ATP transport activity of proteoliposomes containing purified human VNUT was blocked by glibenclamide, carbenoxolone, 18 α-glycyrrhetinic acid, flufenamic acid, arachidonic acid and A438079 without the formation of Δψ (positive inside) as a driving force being affected. Thus, inhibitors of ATP release may inhibit VNUT and subsequent ATP release, since the previous works proved that inhibitors of ATP release blocked VNUT-mediated ATP release at the cell level.
authors.affiliation: Department of Membrane Biochemistry, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Kato
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Omote
authors.forename: Takaaki
authors.initials: T
authors.lastname: Miyaji
mesh_terms: D000255:Adenosine Triphosphate / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016718:Arachidonic Acid / Q000494:pharmacology; D002229:Carbenoxolone / Q000494:pharmacology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005439:Flufenamic Acid / Q000494:pharmacology; D005905:Glyburide / Q000494:pharmacology; D006034:Glycyrrhetinic Acid / Q000494:pharmacology; D006801:Humans; D008081:Liposomes; D008969:Molecular Sequence Data; D033704:Nucleotide Transport Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011725:Pyridines / Q000494:pharmacology; D013777:Tetrazoles / Q000494:pharmacology
chemical_list: C523668:3-(5-(2,3-dichlorophenyl)-1H-tetrazol-1-yl)methylpyridine; D018076:DNA, Complementary; D008081:Liposomes; D033704:Nucleotide Transport Proteins; D011725:Pyridines; C529437:Slc17a9 protein, human; D013777:Tetrazoles; D016718:Arachidonic Acid; D005439:Flufenamic Acid; D000255:Adenosine Triphosphate; D002229:Carbenoxolone; D006034:Glycyrrhetinic Acid; D005905:Glyburide