title: Agrobacterium-mediated transformation of Citrus stem segments and regeneration of transgenic plants.
abstract: A method for Agrobacterium-mediated transformation of Citrus and organogenic regeneration of transgenic plants is reported. Internodal stem segments were co-cultured with Agrobacterium harboring binary vectors that contained the genes for the scorable marker ß-glucuronidase (GUS) and the selectable marker NPT-II. A low but significant percentage (≤ 5%) of the shoots regenerated in the presence of 100 μg/ml kanamycin were GUS(+). Polymerase chain reaction (PCR) analysis confirmed that GUS(+) shoots contained T-DNA. Two plants established in soil were shown to be transgenic by Southern analysis.
authors.affiliation: Fruit Crops Department, Institute of Food and Agricultural Sciences, University of Florida, 32611, Gainesville, FL, USA.
authors.forename: G A
authors.initials: GA
authors.lastname: Moore
authors.forename: C C
authors.initials: CC
authors.lastname: Jacono
authors.forename: J L
authors.initials: JL
authors.lastname: Neidigh
authors.forename: S D
authors.initials: SD
authors.lastname: Lawrence
authors.forename: K
authors.initials: K
authors.lastname: Cline