title: Frequency analyses of active NORs in nuclei of artificially induced triploid fishes.
abstract: The correspondence between increased numbers of both chromosomal and nuclear NORs and artificially induced triploidy in three fish species (rainbow trout, Oncorhynchus mykiss; common carp, Cyprinus carpio; and tench, Tinea tinea) has been confirmed by CMA3 fluorescence and Ag-staining. The frequencies of cell nuclei with one, two and three active NORs, as revealed by Ag-staining, has been analyzed statistically to find the minimum cell number which verifies the increased ploidy level. A minimum sample size of about 80 cells exhibiting three active NORs is sufficient to confirm triploidy in all three species and may be of use for categorising other ploidy-manipulated fish species.
authors.affiliation: Department of Fish Genetics and Breeding, Research Institute of Fish Culture and Hydrobiology, CS-389 25, Vodňany, Czechoslovakia.
authors.forename: M
authors.initials: M
authors.lastname: Flajšhans
authors.forename: P
authors.initials: P
authors.lastname: Ráb
authors.forename: S
authors.initials: S
authors.lastname: Dobosz