title: Respiratory syncytial virus induces leukotriene C4 synthase expression in bronchial epithelial cells.
abstract: OBJECTIVE
  Respiratory syncytial virus (RSV) results in acute wheezing in infants and is frequently associated with recurrent wheezing. Although RSV-induced wheezing clinically resembles that of asthma, corticosteroids are not equivalently effective in RSV-associated wheezing. The study sought to determine the mechanisms of RSV-induced wheezing by establishing an in vitro model of RSV-infected human bronchial epithelial cells (16-HBEC).
  
  
  METHODS
  Leukotriene C4 synthase (LTC4 S) messenger RNA (mRNA) expression in 16-HBEC was detected using fluorescence quantitative polymerase chain reaction, and the relative level of LTC4 S mRNA was expressed as quotient cycle threshold (qCt) based on the threshold cycle number value compared with that of β-actin. Cysteinyl leukotrienes (CysLT) in culture supernatant were measured by enzyme-linked immunosorbent assay. RSV-infected 16-HBEC was incubated with gradient concentration of budesonide (BUD) to assess its effects on LTC4 S expression and CysLT secretion.
  
  
  RESULTS
  RSV infection resulted in increased LTC4 S mRNA expression between 48 and 96 h post-infection. High level of CysLT was detected in the supernatant of RSV-infected 16-HBEC. BUD at concentrations of 10(-10) to 10(-5) mol/L did not significantly alter LTC4 S mRNA expression.
  
  
  CONCLUSIONS
  RSV infection upregulated LTC4 S expression in HBEC leading to increased CysLT secretion. Such induction was not attenuated by BUD, suggesting that CysLT might contribute to the pathogenesis of RSV-induced wheezing.
authors.affiliation: State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Sun
authors.forename: Ai-huan
authors.initials: AH
authors.lastname: Chen
authors.forename: Zi-feng
authors.initials: ZF
authors.lastname: Yang
authors.forename: Jason J
authors.initials: JJ
authors.lastname: Chen
authors.forename: Wen-da
authors.initials: WD
authors.lastname: Guan
authors.forename: Jie-ling
authors.initials: JL
authors.lastname: Wu
authors.forename: Sheng
authors.initials: S
authors.lastname: Qin
authors.forename: Nan-shan
authors.initials: NS
authors.lastname: Zhong
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001249:Asthma / Q000378:metabolism* / Q000473:pathology / Q000821:virology*; D001980:Bronchi / Q000187:drug effects / Q000378:metabolism* / Q000821:virology; D019819:Budesonide / Q000494:pharmacology; D002460:Cell Line; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism* / Q000821:virology; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D012333:RNA, Messenger / Q000378:metabolism; D018077:Receptors, Leukotriene / Q000378:metabolism; D018357:Respiratory Syncytial Virus Infections / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D012136:Respiratory Syncytial Viruses / Q000502:physiology*; D013997:Time Factors
chemical_list: D000893:Anti-Inflammatory Agents; D012333:RNA, Messenger; D018077:Receptors, Leukotriene; D019819:Budesonide; D005982:Glutathione Transferase; C041116:leukotriene-C4 synthase; C083169:leukotriene D4 receptor
keywords: bronchial epithelial cell; corticosteroid; cysteinyl leukotriene; respiratory syncytial virus