title: The behavior of cardiac progenitor cells on macroporous pericardium-derived scaffolds.
abstract: Cardiovascular diseases hold the highest mortality rate among other illnesses which reveals the significance of current limitations in common therapies. Three-dimensional (3D) scaffolds have been utilized as potential therapies for treating heart failure following myocardial infarction (MI). In particular, native tissues have numerous properties that make them potentially useful scaffolding materials for recreating the native cardiac extracellular matrix (ECM). Here, we have developed a pericardium-derived scaffold that mimics the natural myocardial extracellular environment and investigated its properties for cardiac tissue engineering. Human pericardium membranes (PMs) were decellularized to yield 3D macroporous pericardium scaffolds (PSs) with well-defined architecture and interconnected pores. PSs enabled human Sca-1(+) cardiac progenitor cells (CPCs) to migrate, survive, proliferate and differentiate at higher rates compared with decellularized pericardium membranes (DPMs) and collagen scaffolds (COLs). Interestingly, histological examination of subcutaneous transplanted scaffolds after one month revealed low immunological response, enhanced angiogenesis and cardiomyocyte differentiation in PSs compared to DPMs and COLs. This research demonstrates the feasibility of fabricating 3D porous scaffolds from native ECMs and suggests the therapeutic potential of CPC-seeded PSs in the treatment of ischemic heart diseases.
authors.affiliation: Department of Biology, Kharazmi University, Tehran, Iran; Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
authors.forename: Sareh
authors.initials: S
authors.lastname: Rajabi-Zeleti
authors.forename: Sasan
authors.initials: S
authors.lastname: Jalili-Firoozinezhad
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Azarnia
authors.forename: Fahimeh
authors.initials: F
authors.lastname: Khayyatan
authors.forename: Sadaf
authors.initials: S
authors.lastname: Vahdat
authors.forename: Saman
authors.initials: S
authors.lastname: Nikeghbalian
authors.forename: Ali
authors.initials: A
authors.lastname: Khademhosseini
authors.forename: Hossein
authors.initials: H
authors.lastname: Baharvand
authors.forename: Nasser
authors.initials: N
authors.lastname: Aghdami
mesh_terms: D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000737:chemistry; D006801:Humans; D009206:Myocardium / Q000166:cytology*; D010496:Pericardium / Q000737:chemistry*; D016062:Porosity; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
keywords: Cardiac progenitor cells; Decellularization; Macroporous scaffold; Myocardial tissue engineering; Pericardium