title: Effects of calcium, alginate, and calcium-alginate immobilization on growth and tropane alkaloid levels of a stable suspension cell line of Datura innoxia Mill.
abstract: A stabilized two-year old suspension of a Datura innoxia cell line, producing small amounts of tropane alkaloids (scopolamine and hyoscyamine) was used in this study. Calcium alginate immobilization has been shown to be able to increase secondary metabolite (i. e. alkaloid) production. The effects of calcium and ungellified alginate were both beneficial for tropane alkaloid synthesis; a 10mM calcium chloride supply gave the best results, with a 10-fold yield increase.
authors.affiliation: Faculté des Sciences, Laboratoire Androgenèse et Biotechnologie, Université de Picardie Jules Verne, 33, rue St. Leu, 80039, Amiens cx, France.
authors.forename: E
authors.initials: E
authors.lastname: Gontier
authors.forename: B S
authors.initials: BS
authors.lastname: Sangwan
authors.forename: J N
authors.initials: JN
authors.lastname: Barbotin