title: Induction of rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures by yeast extract.
abstract: A transient increase in rosmarinic acid (RA) content in cultured cells of Lithospermum erythrorhizon was observed after addition of yeast extract (YE) to the suspension cultures, reaching a maximum at 24 hr. The highest increase of the RA content (2.5-fold) was obtained when 6-day-old cells in the exponential growth phase were treated with YE. Preceding the induced RA accumulation, phenylalanine ammonia-lyase (PAL) activity increased rapidly, whereas tyrosine aminotransferase (TAT) activity was largely unaffected by the treatment. The incorporation of both (14)C-phenylalanine and (14)C-tyrosine into RA was enhanced in the YE-treated cells, consistent with increased synthesis of the ester.
authors.affiliation: School of Pharmaceutical. Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852, Nagasaki, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Mizukami
authors.forename: T
authors.initials: T
authors.lastname: Ogawa
authors.forename: H
authors.initials: H
authors.lastname: Ohashi
authors.forename: B E
authors.initials: BE
authors.lastname: Ellis