title: Cardiac surgery increases serum concentrations of adipocyte fatty acid-binding protein and its mRNA expression in circulating monocytes but not in adipose tissue.
abstract: Adipocyte fatty acid binding protein (A-FABP) is a novel adipokine involved in the regulation of lipid and glucose metabolism and inflammation. To evaluate its potential role in the development of postoperative hyperglycemia and insulin resistance we assessed A-FABP serum concentrations and mRNA expression in skeletal and myocardial muscle, subcutaneous and epicardial adipose tissue and peripheral monocytes in 11 diabetic and 20 age- and sex-matched non-diabetic patients undergoing elective cardiac surgery. Baseline serum A-FABP did not differ between the groups (31.1+/-5.1 vs. 25.9+/-4.6 ng/ml, p=0.175). Cardiac surgery markedly increased serum A-FABP in both groups with a rapid peak at the end of surgery followed by a gradual decrease to baseline values during the next 48 h with no significant difference between the groups at any timepoint. These trends were analogous to postoperative excursions of plasma glucose, insulin and selected proinflammatory markers. Cardiac surgery increased A-FABP mRNA expression in peripheral monocytes, while no effect was observed in adipose tissue or muscle. Our data suggest that circulating A-FABP might be involved in the development of acute perioperative stress response, insulin resistance and hyperglycemia of critically ill irrespectively of the presence of diabetes mellitus.
authors.affiliation: Third Department of Medicine, First Faculty of Medicine, Charles University, Prague, Czech Republic. mhalu@lf1.cuni.cz.
authors.forename: T
authors.initials: T
authors.lastname: Kotulak
authors.forename: J
authors.initials: J
authors.lastname: Drapalova
authors.forename: M
authors.initials: M
authors.lastname: Lips
authors.forename: Z
authors.initials: Z
authors.lastname: Lacinova
authors.forename: P
authors.initials: P
authors.lastname: Kramar
authors.forename: H
authors.initials: H
authors.lastname: Riha
authors.forename: I
authors.initials: I
authors.lastname: Netuka
authors.forename: J
authors.initials: J
authors.lastname: Maly
authors.forename: J
authors.initials: J
authors.lastname: Blaha
authors.forename: J
authors.initials: J
authors.lastname: Lindner
authors.forename: S
authors.initials: S
authors.lastname: Svacina
authors.forename: M
authors.initials: M
authors.lastname: Mraz
authors.forename: M
authors.initials: M
authors.lastname: Haluzik
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000368:Aged; D015415:Biomarkers / Q000097:blood; D006348:Cardiac Surgical Procedures / Q000009:adverse effects; D050556:Fatty Acid-Binding Proteins / Q000096:biosynthesis*; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*
chemical_list: D015415:Biomarkers; C493410:FABP4 protein, human; D050556:Fatty Acid-Binding Proteins; D012333:RNA, Messenger