title: IL-10 and TNF-alpha polymorphisms in subjects with irritable bowel syndrome in Mexico.
abstract: BACKGROUND
  there has been recent evidence of an alteration in irritable bowel syndrome (IBS) immune regulation, as well as variations in cytokine polymorphisms.
  
  
  OBJECTIVE
  to determine the frequency of the IL-10 (-1082G/A) and TNF-alpha (-308G/A) polymorphisms in subjects with IBS in Mexico.
  
  
  METHODS
  volunteers answered the Rome II Questionnaire and were classified as IBS (n = 45) and controls (n = 92). The IBS subjects were then categorized as IBS-D: 22.2%, IBS-C: 28.9%, and IBS-A/M: 48.9%. The polymorphism frequency among groups was compared.
  
  
  RESULTS
  there were no differences between IBS vs. controls in the frequency of the high (8.9 vs. 18.5%), intermediate (60.0 vs. 57.6%), or low (23.9 vs. 38.9%) producer IL-10 genotypes, p = 0.315. Neither were there differences in the high (0 vs. 1.1%), intermediate (55.4 vs. 43.2%), or low (43.5 vs. 56.8%) producer TNF-alpha genotypes, p = 0.296. However the low producer of IL-10 was more frequent in IBS-D vs. IBS-C vs. IBS-A/M (63.6 vs. 7.1 vs. 33,3%) p = 0.023.
  
  
  CONCLUSIONS
  in this group of volunteers in Mexico, the frequency of the IL-10 (-1082G/A) and TNF-alpha (-308G/A) genotypes was similar in IBS and controls. However, there was a greater frequency of the low producer of IL-10 in those subjects with IBS-D, suggesting a genetic predisposition to abnormal immune regulation due to a lower anti-inflammatory component in this subgroup.
authors.forename: Max
authors.initials: M
authors.lastname: Schmulson
authors.forename: Daniela
authors.initials: D
authors.lastname: Pulido-London
authors.forename: Óscar
authors.initials: Ó
authors.lastname: Rodríguez
authors.forename: Norma
authors.initials: N
authors.lastname: Morales-Rochlin
authors.forename: Rosalinda
authors.initials: R
authors.lastname: Martínez-García
authors.forename: María Concepción
authors.initials: MC
authors.lastname: Gutiérrez-Ruiz
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: López-Alvarenga
authors.forename: Gabriela
authors.initials: G
authors.lastname: Gutiérrez-Reyes
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D043183:Irritable Bowel Syndrome / Q000235:genetics*; D008297:Male; D008800:Mexico; D011110:Polymorphism, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10