title: DNA sequence analysis with droplet-based microfluidics.
abstract: Droplet-based microfluidic techniques can form and process micrometer scale droplets at thousands per second. Each droplet can house an individual biochemical reaction, allowing millions of reactions to be performed in minutes with small amounts of total reagent. This versatile approach has been used for engineering enzymes, quantifying concentrations of DNA in solution, and screening protein crystallization conditions. Here, we use it to read the sequences of DNA molecules with a FRET-based assay. Using probes of different sequences, we interrogate a target DNA molecule for polymorphisms. With a larger probe set, additional polymorphisms can be interrogated as well as targets of arbitrary sequence.
authors.affiliation: University of California, San Francisco - Bioengineering and Therapeutic Sciences, San Francisco, California, USA.
authors.forename: Adam R
authors.initials: AR
authors.lastname: Abate
authors.forename: Tony
authors.initials: T
authors.lastname: Hung
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Sperling
authors.forename: Pascaline
authors.initials: P
authors.lastname: Mary
authors.forename: Assaf
authors.initials: A
authors.lastname: Rotem
authors.forename: Jeremy J
authors.initials: JJ
authors.lastname: Agresti
authors.forename: Michael A
authors.initials: MA
authors.lastname: Weiner
authors.forename: David A
authors.initials: DA
authors.lastname: Weitz
mesh_terms: D001483:Base Sequence; D059014:High-Throughput Nucleotide Sequencing; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*