title: Influence of phosphate ion on the fluorescence of 3-fluorotyrosine.
abstract: 3-Fluorotyrosine fluorescence is quenched effectively by phosphate ions not only by a dynamic but also by a static mechanism owing to H-bond complex formation in ground state. 3-Fluorotyrosine pKa values both in the ground and first excited state (8.3 and 4, respectively) are appreciably lower than those of tyrosine, thus promoting 3-fluorotyrosinate ion formation in the excited state. Additional emission owing to 3-fluorotyrosinate ion (near 350 nm) may be taken erroneously for tryptophan fluorescence.
authors.affiliation: Institute of Bioorganic and Oil Chemistry, Academy of Sciences of Ukraine, Murmanskaja street, 1 Kiev-94, 253660, Ukraine.
authors.forename: S I
authors.initials: SI
authors.lastname: Vdovenko
authors.forename: M T
authors.initials: MT
authors.lastname: Kolycheva
authors.forename: I I
authors.initials: II
authors.lastname: Gerus
authors.forename: V P
authors.initials: VP
authors.lastname: Kukhar