title: A new biphasic test for the detection of Helicobacter pylori in gastric biopsies.
abstract: The objective of this study was to create a biphasic cultural method for the detection of Helicobacter pylori in gastric biopsy specimens. The biphasic systems were made by using a urea agar slant with overlaying broth in a single vessel. Initially, three different liquid media including brain-heart infusion broth, Brucella broth, and Bolton broth were tested for their ability to support the growth of H. pylori. Bolton broth with 10% defibrinated horse blood demonstrated a significant increase in the numbers of H. pylori (p<0.05). The result showed that positive urease was used to concentrate viable H. pylori cells where the numbers of bacteria were 10(5)cfu. In addition, the reliable incubation time was at least 36h. In total, 55 biopsies were comparatively studied using commercial rapid urease test and PCR. Seven samples (12.72%) were positive with H. pylori by the biphasic test. With the CLOtest, 6 (10.91%) samples were positive. In conclusion, the Hp biphasic test achieved more positive samples than did the commercial rapid urease test.
authors.affiliation: School of Allied Health Sciences and Public Health, Walailak University, Tha Sala, Nakhon Si Thammarat 80161, Thailand. Electronic address: sueptrakool.wi@wu.ac.th.
authors.forename: Sueptrakool
authors.initials: S
authors.lastname: Wisessombat
authors.affiliation: Faculty of Medical Technology, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.
authors.forename: Chatruthai
authors.initials: C
authors.lastname: Meethai
authors.affiliation: Faculty of Medical Technology, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.
authors.forename: Souvalak
authors.initials: S
authors.lastname: Hamgo
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D001706:Biopsy; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D005753:Gastric Mucosa / Q000382:microbiology*; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D014510:Urease / Q000032:analysis
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D014510:Urease
keywords: Biphasic media; Gastric biopsy; Helicobacter pylori