title: CD34(low) and SMA(high) represent stromal signature in uterine cervical cancer and are markers for peritumoral stromal remodeling.
abstract: Peritumoral desmoplastic stromal reaction (DSR) with myofibroblastic phenotype may be of prognostic impact in uterine cervical carcinoma. The present study evaluates the immunostaining (CD34 and smooth muscle actin; SMA) of 97 squamous cell cancers. Staining was scored as low/negative (<5% stroma positive), moderate (patchy/focal expression, 5%-50%), or high (diffuse expression throughout peritumoral stroma, >50%) and DSR as negative/weak and moderate/strong. The staining results were correlated to patient survival. Of the cases, 78.3% showed a decreased of CD34 (<5% stromal positivity) and 71.9% an increased SMA staining with more than 50% SMA positive stromal cells. Tumors representing moderate/strong DSR showed a significant decreased CD34 (P=.001) and an increased but not statistically significant SMA staining (P=0.345). Cases with low CD34 and high SMA staining showed reduced 5-year overall survival when compared to cases with high CD34 and low SMA positivity (59.9 vs 81.0%; P=0.025 and 64.6 vs 81.1%; P=0.243). Peritumoral stromal response in cervical carcinoma is immunohistochemically characterized by CD34(low)/SMA(high) and associated reduced overall survival.
authors.affiliation: Institute of Pathology, Division of Breast, Gynecologic & Perinatal Pathology, University of Leipzig, Leipzig, Germany. Electronic address: hornl@medizin.uni-leipzig.de.
authors.forename: Lars-Christian
authors.initials: LC
authors.lastname: Horn
authors.forename: Carolin
authors.initials: C
authors.lastname: Schreiter
authors.forename: Anika
authors.initials: A
authors.lastname: Canzler
authors.forename: Karoline
authors.initials: K
authors.lastname: Leonhardt
authors.forename: Jens
authors.initials: J
authors.lastname: Einenkel
authors.forename: Bettina
authors.initials: B
authors.lastname: Hentschel
mesh_terms: D000199:Actins / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018952:Antigens, CD34 / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D009130:Muscle, Smooth / Q000737:chemistry; D058628:Myofibroblasts / Q000378:metabolism / Q000473:pathology; D011379:Prognosis; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D055815:Young Adult
chemical_list: D000199:Actins; D018952:Antigens, CD34; D014408:Biomarkers, Tumor
keywords: CD34; Cervical carcinoma; Desmoplastic change; Invasion; Myofibroblasts; Prognosis; Smooth muscle actin; Stroma