title: Active drift stabilization in three dimensions via image cross-correlation.
abstract: By monitoring stage drift via the normalized cross-correlation of an image of a stuck bead, obtained in real-time, with an out-of-focus "template" image of a similar immobile bead, stored in memory, we implement a simple approach to actively stabilize drift in all three dimensions for existing video microscopy setups. We demonstrate stability to 0.0062 nm along the Z-axis and 0.0031 nm along the X- and Y-axes for long (100 s) timescales.
authors.affiliation: Department of Physics, Yale University, New Haven, Connecticut 06511, USA.
authors.forename: P K
authors.initials: PK
authors.lastname: Koo
authors.forename: S U
authors.initials: SU
authors.lastname: Setru
authors.forename: S G J
authors.initials: SG
authors.lastname: Mochrie
mesh_terms: D021621:Imaging, Three-Dimensional / Q000379:methods*; D018715:Microscopy, Video / Q000379:methods*; D008863:Microspheres