title: Obesity and chronic stress are able to desynchronize the temporal pattern of serum levels of leptin and triglycerides.
abstract: Disruption of the circadian system can lead to metabolic dysfunction as a response to environmental alterations. This study assessed the effects of the association between obesity and chronic stress on the temporal pattern of serum levels of adipogenic markers and corticosterone in rats. We evaluated weekly weight, delta weight, Lee index, and weight fractions of adipose tissue (mesenteric, MAT; subcutaneous, SAT; and pericardial, PAT) to control for hypercaloric diet-induced obesity model efficacy. Wistar rats were divided into four groups: standard chow (C), hypercaloric diet (HD), stress plus standard chow (S), and stress plus hypercaloric diet (SHD), and analyzed at three time points: ZT0, ZT12, and ZT18. Stressed animals were subjected to chronic stress for 1h per day, 5 days per week, during 80 days. The chronic exposure to a hypercaloric diet was an effective model for the induction of obesity and metabolic syndrome, increasing delta weight, Lee index, weight fractions of adipose tissue, and triglycerides and leptin levels. We confirmed the presence of a temporal pattern in the release of triglycerides, corticosterone, leptin, and adiponectin in naïve animals. Chronic stress reduced delta weight, MAT weight, and levels of triglycerides, total cholesterol, and leptin. There were interactions between chronic stress and obesity and serum total cholesterol levels, between time points and obesity and adiponectin and corticosterone levels, and between time points and chronic stress and serum leptin levels. In conclusion, both parameters were able to desynchronize the temporal pattern of leptin and triglyceride release, which could contribute to the development of metabolic diseases such as obesity and metabolic syndrome.
authors.affiliation: Pain Pharmacology and Neuromodulation, Animal Models Laboratory, Department of Pharmacology, Universidade Federal do Rio Grande do Sul Institute of Basic Health Sciences, Porto Alegre, RS 90050-170, Brazil; Post Graduate Program in Medicine: Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 90035-003, Brazil; Animal Experimentation Unit and Graduate Research Group, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS 90035-003, Brazil.
authors.forename: Carla
authors.initials: C
authors.lastname: de Oliveira
authors.affiliation: Pain Pharmacology and Neuromodulation, Animal Models Laboratory, Department of Pharmacology, Universidade Federal do Rio Grande do Sul Institute of Basic Health Sciences, Porto Alegre, RS 90050-170, Brazil; Animal Experimentation Unit and Graduate Research Group, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS 90035-003, Brazil; Post Graduate Program in Biological Sciences - Physiology, Universidade Federal do Rio Grande do Sul Institute of Basic Health Sciences, Porto Alegre, RS 90050-170, Brazil.
authors.forename: Vanessa Leal
authors.initials: VL
authors.lastname: Scarabelot
authors.affiliation: Pain Pharmacology and Neuromodulation, Animal Models Laboratory, Department of Pharmacology, Universidade Federal do Rio Grande do Sul Institute of Basic Health Sciences, Porto Alegre, RS 90050-170, Brazil; Post Graduate Program in Medicine: Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 90035-003, Brazil; Animal Experimentation Unit and Graduate Research Group, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS 90035-003, Brazil; Laboratório de Bioquímica, Centro de Ciências Básicas da Saúde, Centro Universitário Univates, Lajeado, RS 95900-000, Brazil.
authors.forename: Andressa
authors.initials: A
authors.lastname: de Souza
authors.affiliation: Pain Pharmacology and Neuromodulation, Animal Models Laboratory, Department of Pharmacology, Universidade Federal do Rio Grande do Sul Institute of Basic Health Sciences, Porto Alegre, RS 90050-170, Brazil; Post Graduate Program in Medicine: Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 90035-003, Brazil; Animal Experimentation Unit and Graduate Research Group, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS 90035-003, Brazil.
authors.forename: Cleverson Moraes
authors.initials: CM
authors.lastname: de Oliveira
authors.affiliation: Pain Pharmacology and Neuromodulation, Animal Models Laboratory, Department of Pharmacology, Universidade Federal do Rio Grande do Sul Institute of Basic Health Sciences, Porto Alegre, RS 90050-170, Brazil; Animal Experimentation Unit and Graduate Research Group, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS 90035-003, Brazil; Post Graduate Program in Biological Sciences - Physiology, Universidade Federal do Rio Grande do Sul Institute of Basic Health Sciences, Porto Alegre, RS 90050-170, Brazil.
authors.forename: Liciane Fernandes
authors.initials: LF
authors.lastname: Medeiros
authors.affiliation: Pain Pharmacology and Neuromodulation, Animal Models Laboratory, Department of Pharmacology, Universidade Federal do Rio Grande do Sul Institute of Basic Health Sciences, Porto Alegre, RS 90050-170, Brazil; Animal Experimentation Unit and Graduate Research Group, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS 90035-003, Brazil; Post Graduate Program in Biological Sciences - Physiology, Universidade Federal do Rio Grande do Sul Institute of Basic Health Sciences, Porto Alegre, RS 90050-170, Brazil.
authors.forename: Isabel Cristina
authors.initials: IC
authors.lastname: de Macedo
authors.affiliation: Pain Pharmacology and Neuromodulation, Animal Models Laboratory, Department of Pharmacology, Universidade Federal do Rio Grande do Sul Institute of Basic Health Sciences, Porto Alegre, RS 90050-170, Brazil; Post Graduate Program in Medicine: Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 90035-003, Brazil; Animal Experimentation Unit and Graduate Research Group, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS 90035-003, Brazil.
authors.forename: Paulo Ricardo
authors.initials: PR
authors.lastname: Marques Filho
authors.affiliation: Pain Pharmacology and Neuromodulation, Animal Models Laboratory, Department of Pharmacology, Universidade Federal do Rio Grande do Sul Institute of Basic Health Sciences, Porto Alegre, RS 90050-170, Brazil; Post Graduate Program in Medicine: Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 90035-003, Brazil; Animal Experimentation Unit and Graduate Research Group, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS 90035-003, Brazil.
authors.forename: Stefania Giotti
authors.initials: SG
authors.lastname: Cioato
authors.affiliation: Pain Pharmacology and Neuromodulation, Animal Models Laboratory, Department of Pharmacology, Universidade Federal do Rio Grande do Sul Institute of Basic Health Sciences, Porto Alegre, RS 90050-170, Brazil; Post Graduate Program in Medicine: Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 90035-003, Brazil.
authors.forename: Wolnei
authors.initials: W
authors.lastname: Caumo
authors.affiliation: Pain Pharmacology and Neuromodulation, Animal Models Laboratory, Department of Pharmacology, Universidade Federal do Rio Grande do Sul Institute of Basic Health Sciences, Porto Alegre, RS 90050-170, Brazil; Post Graduate Program in Medicine: Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 90035-003, Brazil; Animal Experimentation Unit and Graduate Research Group, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS 90035-003, Brazil; Post Graduate Program in Biological Sciences - Physiology, Universidade Federal do Rio Grande do Sul Institute of Basic Health Sciences, Porto Alegre, RS 90050-170, Brazil. Electronic address: iracitorres@gmail.com.
authors.forename: Iraci L S
authors.initials: IL
authors.lastname: Torres
mesh_terms: D052242:Adiponectin / Q000097:blood; D052436:Adipose Tissue, White / Q000473:pathology; D050154:Adiposity; D000818:Animals; D002784:Cholesterol / Q000097:blood; D002908:Chronic Disease; D002940:Circadian Rhythm; D003345:Corticosterone / Q000097:blood; D020738:Leptin / Q000097:blood*; D008297:Male; D009765:Obesity / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000097:blood*; D014280:Triglycerides / Q000097:blood*; D015430:Weight Gain
chemical_list: D052242:Adiponectin; D020738:Leptin; D014280:Triglycerides; D002784:Cholesterol; D003345:Corticosterone
keywords: Chronic stress; Hypercaloric diet; Metabolic syndrome; Obesity; Temporal pattern