title: Optimizing the production of transformed pea (Pisum sativum L.) callus using disarmed Agrobacterium tumefaciens strains.
abstract: For optimization of the transformation procedure with Pisum sativum L. stern explant callus was used to test the effect of disarmed Agrobacterium tumefaciens strains, cocultivation procedures (preconditioning of explants; use of Nicotiana tabacum L. nurse cultures), duration of cocultivation (2, 3 or 4 days), and agents for selection (kanamycin or hygromycin). The succinamopine strain EHA101(pBI1042) produced the highest percentage of transformed calli (77%) when used in conjunction with tobacco nurse culture during four days of cocultivation. Using this strain, kanamycin (76%) and hygromycin (77%) were equally effective selective agents, but for strain LBA4404(pBI1042) percentage of transformed calli was higher for hygromycin (63%) than for kanamycin (17%). The procedures and strains shown to be optimal for transformation of pea callus will now be complemented by a pea regeneration system.
authors.affiliation: Plant Biotechnology Institute, NRC, 110 Gymnasium Road, S7N OW9, Saskatoon, Saskatchewan, Canada.
authors.forename: M M
authors.initials: MM
authors.lastname: Lulsdorf
authors.forename: H
authors.initials: H
authors.lastname: Rempel
authors.forename: J A
authors.initials: JA
authors.lastname: Jackson
authors.forename: D S
authors.initials: DS
authors.lastname: Baliski
authors.forename: S L
authors.initials: SL
authors.lastname: Hobbs