title: Identification of Ccr4-not complex components as regulators of transition from partial to genuine induced pluripotent stem cells.
abstract: Somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) by defined factors. However, substantial cell numbers subjected to iPSC induction stray from the main reprogramming route and are immortalized as partial iPSCs. These partial iPSCs can become genuine iPSCs by exposure to the ground state condition. However, such conversion is only possible for mouse partial iPSCs, and it is not applicable to human cells. Moreover, the molecular basis of this conversion is completely unknown. Therefore, we performed genome-wide screening with a piggyBac vector to identify genes involved in conversion from partial to genuine iPSCs. This screening led to identification of Cnot2, one of the core components of the Ccr4-Not complex. Subsequent analyses revealed that other core components, Cnot1 and Cnot3, also contributed to the conversion. Thus, our data have uncovered a novel role of core components of the Ccr4-Not complex as regulators of transition from partial to genuine iPSCs.
authors.affiliation: 1 Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical University , Yamane Hidaka, Saitama, Japan .
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Kamon
authors.forename: Miyuki
authors.initials: M
authors.lastname: Katano
authors.forename: Keiko
authors.initials: K
authors.lastname: Hiraki-Kamon
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Hishida
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nakachi
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Mizuno
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Okazaki
authors.forename: Ayumu
authors.initials: A
authors.lastname: Suzuki
authors.forename: Masataka
authors.initials: M
authors.lastname: Hirasaki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ueda
authors.forename: Masazumi
authors.initials: M
authors.lastname: Nishimoto
authors.forename: Hidemasa
authors.initials: H
authors.lastname: Kato
authors.forename: Akihiko
authors.initials: A
authors.lastname: Okuda
mesh_terms: D000818:Animals; D002460:Cell Line; D002469:Cell Separation; D002999:Clone Cells; D015536:Down-Regulation; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D063990:Gene Ontology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D046912:Multiprotein Complexes / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D054398:Receptors, CCR4 / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D000076183:Tripartite Motif-Containing Protein 28
chemical_list: C569362:CNOT3 protein, mouse; D046912:Multiprotein Complexes; D009687:Nuclear Proteins; D054398:Receptors, CCR4; D012097:Repressor Proteins; D014157:Transcription Factors; C103190:Trim28 protein, mouse; D000076183:Tripartite Motif-Containing Protein 28