title: Cnuella takakiae gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from Takakia lepidozioides.
abstract: A Gram-staining-negative, rod-shaped and non-spore-forming bacterium, designated strain RG1-1(T), was isolated from Takakia lepidozioides collected from Gawalong glacier in Tibet, China, and characterized by using a polyphasic taxonomic approach. The predominant fatty acids of strain RG1-1(T) were iso-C(15 : 0) (19.8%), summed feature 3 (C(16 : 1)ω7c and/or C(16 : 1)ω6c, 17.0%), C(16 : 0 (9.9)%) and iso-C(17 : 0) 3-OH (9.4%); its major polar lipids were phosphatidylethanolamine, four unidentified aminolipids, one unidentified phospholipid, one unidentified aminoglycolipid, one unidentified glycolipid, and three unidentified lipids. Strain RG1-1(T) contained MK-7 as the dominant menaquinone, and the G+C content of its genomic DNA was 49.1 mol%. Strain RG1-1(T) exhibited the highest 16S rRNA gene sequence similarity (91.8%) with Flavisolibacter ginsengiterrae Gsoil 492(T) and Flavisolibacter ginsengisoli Gsoil 643(T). Phylogenetic analysis showed that strain RG1-1(T) was a member of the family Chitinophagaceae, phylum Bacteroidetes. On the basis of 16S rRNA gene sequence analysis, and phenotypic and chemotaxonomic data, strain RG1-1(T) is considered to represent a novel species of a novel genus, for which the name Cnuella takakiae gen. nov., sp. nov. is proposed. The type strain is RG1-1(T) ( = CGMCC 1.12492(T) = DSM 26897(T)).
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Ran
authors.initials: R
authors.lastname: Zhao
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Xin Yao
authors.initials: XY
authors.lastname: Chen
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Xue Dong
authors.initials: XD
authors.lastname: Li
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Tian
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Bi He
authors.initials: BH
authors.lastname: Kong
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Zhi Ling
authors.initials: ZL
authors.lastname: Chen
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Yan Hong
authors.initials: YH
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D044002:Bryophyta / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D046448:Ice Cover; D008969:Molecular Sequence Data; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D018609:Tibet; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7