title: The effects of hypoxia, in vivo, on red blood cell β-adrenoceptors in the rainbow trout, Oncorhynchus mykiss.
abstract: Rainbow trout, Oncorhynchus mykiss, were exposed to 48h of environmental hypoxia (water partial pressure of oxygen = 8.0 kPa) at either 5 or 15°C. Blood was sampled during hypoxia via a dorsal aorta cannula to measure arterial blood partial pressure of oxygen and plasma catecholamine concentrations. After 48h, the number (Bmax) and dissociation constant (Kd) of red blood cell surface β-adrenoceptors were determined using a radioligand-displacement binding assay. At 5°C, plasma catecholamine levels were elevated at 24h whereas at 15°C, levels were elevated at 48h. At either temperature, following 48h of hypoxia, there was no change in Bmax or Kd of red blood cell surface β-adrenoceptors, compared to normoxic control fish. This study demonstrates that chronic exposure to moderate hypoxia does not affect the number or affinity of cell surface β-adrenoceptors on trout red blood cells.
authors.affiliation: Department of Biology, University of Ottawa, 30 Marie Curie, Ottawa, Ontario, Canada, K1N 6N5.
authors.forename: S G
authors.initials: SG
authors.lastname: Reid
authors.forename: S F
authors.initials: SF
authors.lastname: Perry