title: Coculture of peripheral blood CD34+ cell and mesenchymal stem cell sheets increase the formation of bone in calvarial critical-size defects in rabbits.
abstract: The reconstruction of large bony defects remains a clinical challenge, and angiogenesis and neovascularisation are being given more attention in bone tissue engineering. In this study we cocultured peripheral blood CD34+ cells (PB-CD34+ cells), an endothelial progenitor cell/haematopoietic stem cell-enriched population, with bone marrow-derived mesenchymal stem cells (MSC) to investigate their potential for bony regeneration. Cocultured cells showed better osteogenic differentiation than MSC alone in vitro. The cocultured cells and MSC sheets were also composited with hydroxyapatite and implanted in calvarial critical-size defects in rabbits. The rabbits were killed before microcomputed tomographic (MicroCT) and histological analysis. The results showed that cocultured cell composites had promoted bony regeneration more efficiently by 8 weeks after implantation. Our results indicate that the coculture of PB-CD34+ cells and MSC increases bony regeneration in calvarial critical-size defects in rabbits, and provide a new promising therapeutic strategy to aid skeletal healing.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China; Department of Stomatology, PLA 252 Hospital, Baoding 071000, China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Orthodontics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Oral and Maxillofacial Surgery, Lanzhou General Hospital, Lanzhou Command of PLA, Lanzhou 730000, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Cao
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Zhaoyu
authors.initials: Z
authors.lastname: Ju
authors.affiliation: Department of Oral and Maxillofacial Surgery, Lanzhou General Hospital, Lanzhou Command of PLA, Lanzhou 730000, China.
authors.forename: Dongyang
authors.initials: D
authors.lastname: Ma
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China. Electronic address: liuyanpu8@163.com.
authors.forename: Yanpu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China. Electronic address: zjrfmmu@163.com.
authors.forename: Junrui
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001847:Bone Diseases / Q000473:pathology / Q000601:surgery*; D001861:Bone Regeneration / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D018920:Coculture Techniques; D003399:Craniotomy / Q000379:methods; D017886:Durapatite / Q000737:chemistry; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D010006:Osteoblasts / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology*; D011817:Rabbits; D011897:Random Allocation; D012886:Skull / Q000473:pathology / Q000601:surgery*; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: D017886:Durapatite; D000469:Alkaline Phosphatase
keywords: Bone regeneration; Cell sheet transplantation; Co-culture; Mesenchymal stem cells; Peripheral blood CD34+ cells; Rabbit calvarial critical-size defect