title: Detection of Enteroviruses in Water Samples from Yopougon, Côte d'Ivoire by Cell Culture and Polymerase Chain Reaction.
abstract: The objective of this study was to compare sensitivities of enterovirus isolation from wastewater in different cell lines as well as to compare the sensitivity and specificity of isolation in cell culture with direct detection by reverse transcription polymerase chain reaction (RT-PCR). Sixty-eight samples of wastewaters were collected between September 2008 and January 2009 in Yopougon, Abidjan. Enteroviruses were concentrated according to World Health Organization recommendations. Viruses were inoculated into various cell lines while direct RT-PCR was performed on water concentrates. The buffalo green monkey kidney cell line was the most sensitive with 58.8 % of viral isolation. This was followed by the rhabdomyosarcoma cell line with sensitivity of 51.6 %, with human epidermoid carcinoma cell line showing sensitivity of 50 % and fibroblastic cells derived from transgenic mice LTK-1 (L20B) cell showing 23.50 % sensitivity. However, a lower specificity of 2.9 % was observed with the L20B cell line. 44.1 % of the samples were positive by direct RT-PCR detection while 51.47 % samples were positive by using RT-PCR on infected cell cultures. No difference in percentage positivity was observed using RT-PCR on infected tissue culture isolates or using RT-PCR directly on wastewater samples.
authors.affiliation: Unité des virus du système nerveux, Département virus épidémique, Institut Pasteur de Cote d'Ivoire, BP 490, Abidjan 01, Ivory Coast. momoujulien@yahoo.fr.|UFR sciences et gestion de l'environnement, Laboratoire des sciences de l'environnement université Nangui Abrogoua, BP 801, Abidjan 02, Ivory Coast. momoujulien@yahoo.fr.
authors.forename: K J
authors.initials: KJ
authors.lastname: Momou
authors.affiliation: UFR des sciences médicales de Bouaké, Université de Bouaké, Abidjan, Ivory Coast.
authors.forename: C
authors.initials: C
authors.lastname: Akoua-Koffi
authors.affiliation: Département bactériologie virologie, Institut Pasteur de Cote d'Ivoire, BP V153, Abidjan 01, Ivory Coast.
authors.forename: M
authors.initials: M
authors.lastname: Dosso
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D007560:Cote d'Ivoire; D004770:Enterovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D004769:Enterovirus Infections / Q000821:virology; D006801:Humans; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D062065:Wastewater / Q000821:virology*
chemical_list: D062065:Wastewater
keywords: Cell cultures; Enteroviruses; RT-PCR; Sensitivity