title: Light microscopy mapping of connections in the intact brain.
abstract: Mapping of neural connectivity across the mammalian brain is a daunting and exciting prospect. Current approaches can be divided into three classes: macroscale, focusing on coarse inter-regional connectivity; mesoscale, involving a finer focus on neurons and projections; and microscale, reconstructing full details of all synaptic contacts. It remains to be determined how to bridge the datasets or insights from the different levels of study. Here we review recent light-microscopy-based approaches that may help in integration across scales.
authors.affiliation: Institute of Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA, USA.
authors.forename: Sung-Yon
authors.initials: SY
authors.lastname: Kim
authors.forename: Kwanghun
authors.initials: K
authors.lastname: Chung
authors.forename: Karl
authors.initials: K
authors.lastname: Deisseroth
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology*; D001931:Brain Mapping; D006801:Humans; D008853:Microscopy; D009434:Neural Pathways