title: An extensive hairy root production precedes shoot regeneration in protoplast-derived calli of cauliflower (Brassica oleracea var. botrytis).
abstract: Protoplasts isolated from mesophyll tissues of cauliflower (Brassica oleraceavar. botrytis) were induced to divide in culture, with 2% of them producing calli. Upon transfer to a regeneration medium containing a low auxin/cytokinin balance (0.02mg/l 1-naphthaleneacetic and 2mg/l 6-benzylaminopurine), they displayed an extensive production of hairy roots before the regeneration of shoots. Negative effects of root differentiation on the subsequent caulogenesis by such calli were not observed, since 97% of the calli regenerated hairy roots and 93% gave shoots.
authors.affiliation: Lab. Amel. Pl. et Biotechnologies, ENITHP, 2 rue le Nôtre, 49045, Angers, France.
authors.forename: N
authors.initials: N
authors.lastname: Delpierre
authors.forename: J
authors.initials: J
authors.lastname: Boccon-Gibod