title: Production of disease-free encapsulated buds of Zingiber officinale Rosc.
abstract: Shoot buds of ginger were successfully encapsulated in 4% sodium alginate gel. Encapsulated buds were germinated in vitro to form roots and shoots. In vitro germination (emergence of sprouts) of encapsulated buds ranged from 16.7% to 81.8% on different media after 5 weeks of incubation. Normal plantlets with an average shoot length of 2.3 cm and 1.7 cm root length were successfully transplanted into unsterilized soil without any hardening process. These plantlets showed no symptoms of ginger yellows disease and the causal fungal pathogen failed to grow out on culture media (used as a diagnostic test).
authors.affiliation: Biotechnology Centre, Himachal Pradesh Krishi Vishvavidyalaya, 176062, Palampur, India.
authors.forename: T R
authors.initials: TR
authors.lastname: Sharma
authors.forename: B M
authors.initials: BM
authors.lastname: Singh
authors.forename: R S
authors.initials: RS
authors.lastname: Chauhan