title: Novel analgesic peptides from the tree frog of Hyla japonica.
abstract: Two novel analgesic peptides (Analgesin-HJ, FWPVI-NH2 and Analgesin-HJ(I5T), FWPVT-NH2) were identified from the skin of the tree frog, Hyla japonica. There are 171 amino acid residues in the precursor encoding analgesin-HJs. The precursor contains 10 copies of mature peptide, which include 9 copies of analgesin-HJ and one copy of analgesin-HJ(I5T). Results from analgesic experiments using mice models including abdominal writhing induced by acetic acid, formalin-induced paw licking, and thermal pain test indicated that this two peptides exerted comparable analgesic activities with morphine. In addition, they had ability to inhibit inflammatory factor secretion induced by lipopolysaccharides (LPS). Considering their easy production, storage, transfer and potential analgesic activity, analgesin-HJs might be exciting leading compounds or templates for the development of novel analgesic agent. In addition, this study might facilitate to understand skin defensive mechanism of amphibians.
authors.affiliation: Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, Hebei, China.
authors.forename: Zhengtao
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China; Graduate University of Chinese Academy of Sciences, Beijing 100009, China.
authors.forename: Han
authors.initials: H
authors.lastname: Liu
authors.affiliation: Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: He
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China.
authors.forename: Jieqiong
authors.initials: J
authors.lastname: Jin
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Che
authors.affiliation: Huangnihe National Nature Reserve, Tonghua 134000, Jilin, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Li
authors.affiliation: Yunnan Clinical Research Center of Breast Cancer, The Third Affiliated Hospital of Kunming Medical College, Kunming 650032, China.
authors.forename: Wenlin
authors.initials: W
authors.lastname: Chen
authors.affiliation: Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China; Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China. Electronic address: rlai72@njau.edu.cn.
authors.forename: Ren
authors.initials: R
authors.lastname: Lai
authors.affiliation: College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, Hebei, China. Electronic address: liujingze@mail.hebtu.edu.cn.
authors.forename: Jingze
authors.initials: J
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001001:Anura; D001483:Base Sequence; D002460:Cell Line; D004353:Drug Evaluation, Preclinical; D005260:Female; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D059225:Nociception / Q000187:drug effects; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D011498:Protein Precursors / Q000737:chemistry / Q000494:pharmacology; D020449:Repetitive Sequences, Amino Acid; D012867:Skin / Q000737:chemistry*
chemical_list: D029845:Amphibian Proteins; D000894:Anti-Inflammatory Agents, Non-Steroidal; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D010446:Peptide Fragments; D011498:Protein Precursors; C000592212:analgesin-HJ(I5T), Hyla japonica; C000592211:analgesin-HJ, Hyla japonica
keywords: Amphibian; Analgesic; Defensive; Peptide; Skin