title: Note: Four-port microfluidic flow-cell with instant sample switching.
abstract: A simple device for high-speed microfluidic delivery of liquid samples to a surface plasmon resonance sensor surface is presented. The delivery platform is comprised of a four-port microfluidic cell, two ports serve as inlets for buffer and sample solutions, respectively, and a high-speed selector valve to control the alternate opening and closing of the two outlet ports. The time scale of buffer/sample switching (or sample injection rise and fall time) is on the order of milliseconds, thereby minimizing the opportunity for sample plug dispersion. The high rates of mass transport to and from the central microfluidic sensing region allow for SPR-based kinetic analysis of binding events with dissociation rate constants (k(d)) up to 130 s(-1). The required sample volume is only 1 μL, allowing for minimal sample consumption during high-speed kinetic binding measurement.
authors.affiliation: School of Electrical, Computer, and Energy Engineering, Arizona State University, Tempe, Arizona 85287, USA.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: MacGriff
authors.forename: Shaopeng
authors.initials: S
authors.lastname: Wang
authors.forename: Nongjian
authors.initials: N
authors.lastname: Tao
mesh_terms: D008027:Light; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*