title: Investigation of poly (ADP-ribose) polymerase-1 genetic variants as a possible risk for allergic rhinitis.
abstract: Recent studies point toward the involvement of poly (ADP-ribose) polymerase-1 (PARP-1) in the pathogenesis of allergic airway inflammation, such as asthma and allergic rhinitis (AR). It has been suggested that inhibition of PARP-1 provides significant protection against systemic or tissue inflammation in animal models. The objective of this study was to investigate whether single-nucleotide polymorphisms of PARP-1 gene are associated with genetic susceptibility to AR. We studied the effect of promoter variations and Val762Ala polymorphism of the PARP-1 gene on the risk for developing AR in a case-control association study with 110 RA patients and 130 control subjects in a Turkish population. The polymorphisms of 410 C/T, -1672G/A, and Val762Ala in the PARP-1 gene were analyzed using the polymerase chain reaction-restriction fragment length polymorphism method. Haplotype analysis of these groups was also performed. The results were statistically analyzed by calculating the odds ratio (OR) and their 95% confidence intervals using χ(2) tests. The heterozygote genotype of the promoter polymorphism (-1672) was significantly found to be associated with susceptibility to AR (OR: 0.56) among the tested single-nucleotide polymorphisms. Haplotypes of PARP-1 -410, -1672, and 762 were not associated with an increased risk for AR. These results raise the possibility that the promoter (-1672) polymorphism of the PARP-1 gene may be a risk factor for AR.
authors.affiliation: 1 Department of Medical Biology, Cerrahpasa Faculty of Medicine, Istanbul University , Istanbul, Turkey .
authors.forename: Ahmet
authors.initials: A
authors.lastname: Ozaydin
authors.forename: Fahri
authors.initials: F
authors.lastname: Akbas
authors.forename: Fadlullah
authors.initials: F
authors.lastname: Aksoy
authors.forename: Yavuz Selim
authors.initials: YS
authors.lastname: Yildirim
authors.forename: Hasan
authors.initials: H
authors.lastname: Demirhan
authors.forename: Funda
authors.initials: F
authors.lastname: Karakurt
authors.forename: Arzuhan
authors.initials: A
authors.lastname: Koc
authors.forename: Ilhan
authors.initials: I
authors.lastname: Onaran
authors.forename: Gonul
authors.initials: G
authors.lastname: Kanigur Sultuybek
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D020022:Genetic Predisposition to Disease; D006801:Humans; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D065631:Rhinitis, Allergic; D012221:Rhinitis, Allergic, Perennial / Q000235:genetics*
chemical_list: D017931:DNA Primers; D011065:Poly(ADP-ribose) Polymerases