title: Investigation on the interaction between endocrine disruptor triphenyltin with human serum albumin.
abstract: The interaction between triphenyltin (TPT) and human serum albumin (HSA) in physiological buffer (pH=7.4) was investigated by the fluorescence quenching technique. The results of fluorescence titration revealed that TPT could strongly quench the intrinsic fluorescence of HSA through a static quenching procedure. The apparent binding constants K and number of binding sites n of TPT with HSA were 2.51×10(3) and 0.96 at 298 K which were obtained by the fluorescence quenching method. The thermodynamic parameters enthalpy change (ΔH), entropy change (ΔS) were positive, which indicated that the interaction of TPT with HSA was driven mainly by hydrophobic forces. The process of binding was a spontaneous process in which Gibbs free energy change was negative. The distance r between donor (HSA) and acceptor (TPT) was calculated to be 3.13 nm based on Forster's non-radiative energy transfer theory. The results of synchronous fluorescence, three-dimensional fluorescence and circular dichroism (CD) spectra showed that the triphenyltin induced conformational changes of HSA.
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China. Electronic address: gengbing2000@126.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Geng
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Xue
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ye
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Hongna
authors.initials: H
authors.lastname: Li
authors.affiliation: Shenyang University of Chemical Technology, Shenyang 110142, PR China.
authors.forename: Jinbao
authors.initials: J
authors.lastname: Wu
mesh_terms: D052244:Endocrine Disruptors / Q000378:metabolism* / Q000633:toxicity; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D009947:Organotin Compounds / Q000378:metabolism* / Q000633:toxicity; D011485:Protein Binding; D011487:Protein Conformation / Q000187:drug effects; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
chemical_list: D052244:Endocrine Disruptors; D009947:Organotin Compounds; D012709:Serum Albumin; C030665:triphenyltin
keywords: Fluorescence spectra; Human serum albumin (HSA); Toxicity; Triphenyltin (TPT)