title: [Cloning and bioinformation analysis of flavone synthase II gene of Erigeron breviscapus].
abstract: OBJECTIVE
  Erigeron breviscapus is a medicinal plant with the most developmental potential in Yunnan province, which is belongs to Erigeron genus of Compositae family. Scutellarin, the main active component of Erigeron breviscapus is one of flavone 7-O-glucuronide derivatives, its biosynthesis pathway is still not clear.
  
  
  METHODS
  Full length cDNA encoding flavone syhthase II gene in E. breviscapus was cloned in this study using R-PCR, 3'-RACE and 5'-RACE.
  
  
  RESULTS
  The opening reading frame of FS II cDNA of E. breviscapus is 1 557 bp long and encoding 518 amino acids, designed as EbFS II, which is highly homologous with FS II of Compositae species, like Callistephus chinensis, Cynara cardunculus var. scolymus, Gerbera hybrida, Dahlia pinnata and Lobelia erinus.
  
  
  CONCLUSIONS
  Phylogenetic analysis showed that EbFS II might has the function of directly converting flavanone to flavone.
authors.affiliation: Yunnan Research Center on Good Agricultural Practice for Dominant Chinese Medicinal Materials, Yunnan Agricultural University, Kunming 650201, China. ghuizhang@hotmail.com
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Zhang
authors.forename: Cui-Ting
authors.initials: CT
authors.lastname: Li
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jun-Wen
authors.initials: JW
authors.lastname: Chen
authors.forename: Jian-Wen
authors.initials: JW
authors.lastname: Yang
authors.forename: Sheng-Chao
authors.initials: SC
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular / Q000379:methods; D019295:Computational Biology / Q000379:methods; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism*; D049919:Erigeron / Q000201:enzymology* / Q000235:genetics*; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010946:Plants, Medicinal / Q000201:enzymology / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D003577:Cytochrome P-450 Enzyme System; C114032:cytochrome P-450 CYP93B1 (Glycyrrhiza echinata)