title: Advances in somatic embryogenesis and plant production of black locust (Robinia pseudoacacia L.).
abstract: Black locust (Robinia pseudoacacia L.) immature seeds of different developmental stages were tested for the ability to initiate embryogenic cultures. Best results (average of 12% embryogenic cultures) were obtained when seeds collected 2-3 weeks post-anthesis were cultured for 3 weeks on modified Finer and Nagasawa medium containing 2,4-D (45-90 μM) and BA (2.2 μM) and then transferred to the same medium without growth regulators. Embryo conversion was obtained from naked or encapsulated somatic embryos derived from a long-term embryogenic line. Without cold treatment, 71% of naked embryos and 41% of the encapsulated embryos converted into plants. Fifteen days of cold treatment increased conversion rates up to 95% for naked embryos and 80% for encapsulated embryos. Recovered plantlets were acclimatized and grown in the greenhouse.
authors.affiliation: D.B. Warnell School of Forest Resources, The University of Georgia, 30602-2152, Athens, GA, USA.
authors.forename: I
authors.initials: I
authors.lastname: Arrillaga
authors.forename: J J
authors.initials: JJ
authors.lastname: Tobolski
authors.forename: S A
authors.initials: SA
authors.lastname: Merkle