title: Iridoid glycosides extracted from zhizi (fructus gardeniae) decrease collagen-induced platelet aggregation and reduce carotid artery thrombosis in an in vivo rat model.
abstract: OBJECTIVE
  To investigate the anti-platelet aggregation and antithrombotic effects in rats of iridoid glycosides extracted from Zhizi (Fructus Gardeniae).
  
  
  METHODS
  The present study evaluated the antithrombotic activity of iridoid glycosides (IGs) in a rat model of carotid artery thrombosis. The effects on coagulation, such as thromboplastin time (APTT), thrombin time (TT) and prothrombin time (PT), and the effect on collagen-induced platelet aggregation in vivo were investigated. Rats were intragastrically administered IGs (50, 100 or 200 mg/ kg) twice daily for 3 days.
  
  
  RESULTS
  IGs were shown for the first time to have an antithrombotic action through the inhibition of platelet aggregation, with little effect on the coagulation time of peripheral blood. Our results also showed that IGs may significantly and dose-dependently reduce arterial thrombus load in a model of carotid artery thrombosis and inhibit collagen-induced platelet aggregation in rats. IGs (100 or 200 mg/kg) had no significant effect on APTT and PT, but did lengthen TT at a higher dose.
  
  
  CONCLUSIONS
  These data, together with the previously reported neuroprotective effects of IGs in rats with cerebral ischemia, suggest that the antithrombotic action of IGs may potentially contribute to the treatment of cerebral ischemic diseases, including cerebral apoplexy.
authors.affiliation: Department of Clinical Laboratory, The First College of Clinical Medical Science of China Three Gorges University, Yichang Central People's Hospital, Yichang 443003, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Qunxing
authors.initials: Q
authors.lastname: Wang
authors.forename: Chunhua
authors.initials: C
authors.lastname: Luo
authors.forename: Chao
authors.initials: C
authors.lastname: Tan
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Yuan
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D002341:Carotid Artery Thrombosis / Q000188:drug therapy* / Q000503:physiopathology; D003094:Collagen / Q000009:adverse effects; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006801:Humans; D057888:Iridoid Glucosides / Q000008:administration & dosage*; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019666:Rubiaceae / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D057888:Iridoid Glucosides; D003094:Collagen