title: Identification of a heparin-binding, mesoderm-inducing peptide in the swim-bladder of the red seabream, Pagrus major: a probable fish fibroblast growth factor.
abstract: The brain, pituitary, liver, ovary and swim-bladder of the red seabream (Pagrus major) were screened for the presence of a growth factor capable of stimulating the proliferation of BHK-21 cells, and the swimbladder extract was found to exert the most powerful activity. Swim-bladder derived growth factor (SBDGF) with heparin-binding ability was isolated by heparin-affinity and size-exclusion chromatography using heparin-Sepharose CL-6B and Superose 12, respectively. The relative molecular mass of SBDGF was 22,500 and the isoelectric point was 9.4. The growth of mammalian fibroblasts (BALB/3c 3T3, BHK-21) and endothelial cells (CPAE) was markedly stimulated by SBDGF. Its growth-promoting activity was also effective in fish fibroblasts (BF-2 and RTG-2), and was heat- and acid-labile. In the mesoderm-induction assay, explants of presumptive ectoderm dissected from Xenopus laevis blastulae (stage 9) were incubated with SBDGF. SBDGF induced ventral mesoderm in the explants, including hemocyte-like cells, mesenchyme and coelomic epithelium. These properties suggest that SBDGF is a fish fibroblast growth factor and that the swim-bladder is the source.
authors.affiliation: Department of Nutrition, National Research Institute of Aquaculture, Nansei, Mie, 516-01, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Kurokawa
authors.forename: M
authors.initials: M
authors.lastname: Asashima