title: [Effect of osthole on bone metabolism in rat femoral tissues in vitro].
abstract: OBJECTIVE
  To investigate the effect of osthole on bone metabolism in rat femoral tissues in vitro.
  
  
  METHODS
  The rat femoral tissues were isolated in vitro. The optimal concentrations of ostehole (1×10(-5) mol/L) and estradiol (1×10(-8) mol/L) (the positive control) were selected by alkaline phosphatase activity (ALP). The ALP and calcium levels were detected by commmerical regents, and the expressions of osteoprotegerin, receptor activator of nuclear factor-κB ligand, runx-related gene 2, and bone morphogenetic protein-2 mRNA were determined by real-time reverse transcription-polymerase chain reaction.
  
  
  RESULTS
  The osthole (1×10(-5) mol/L) significantly increased the activity of ALP, calcium level as well as the expressions of osteoprotegerin, receptor activator of nuclear factor-κB ligand, runx-related gene-2 and bone morphogenetic protein-2 mRNA in rat femoral tissues in vitro.
  
  
  CONCLUSIONS
  Osthole can improve calcium level and ALP activity and regulate the bone metabolism-related genes in rat femoral tissues.
authors.affiliation: Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.forename: Bao-feng
authors.initials: BF
authors.lastname: Ge
authors.forename: Ping
authors.initials: P
authors.lastname: Zhen
authors.forename: Xiao-ni
authors.initials: XN
authors.lastname: Ma
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Yan
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Guo
authors.forename: Kui
authors.initials: K
authors.lastname: Cheng
authors.forename: Yu-Hai
authors.initials: YH
authors.lastname: Gao
authors.forename: Wen-Gui
authors.initials: WG
authors.lastname: Shi
authors.forename: Ke-ming
authors.initials: KM
authors.lastname: Chen
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D003374:Coumarins / Q000494:pharmacology*; D005269:Femur / Q000187:drug effects* / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D053244:Osteoprotegerin / Q000378:metabolism; D053245:RANK Ligand / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D050659:Core Binding Factor Alpha 1 Subunit; D003374:Coumarins; D053244:Osteoprotegerin; D053245:RANK Ligand; C493717:Runx2 protein, rat; C506032:Tnfrsf11b protein, rat; D000469:Alkaline Phosphatase; D002118:Calcium; C046627:osthol