title: Mechanism-based anti-anxiety effects of polysaccharides extracted from shudihuang (radix rehmanniae preparata) by two-dimensional electrophoresis analysis in rat hippocampus proteins.
abstract: OBJECTIVE
  To investigate mechanism-based anti-anxiety effects of Shudihuang (Radix Rehmanniae Preparata) polysaccharides (RRPPs) through two-dimensional electrophoresis (2-DE) analysis with mass spectrometry (MS) of hippocampus proteins in rats treated with monosodium L-glutamate (MSG).
  
  
  METHODS
  MSG (4 g/kg) or normal saline (NS) was injected subcutaneously into infant male rats on days 2, 4, 6, 8, 10 after birth. MSG-treated rats at 8 weeks old were given NS, diazepam, or RRPPs daily for seven consecutive days by intragastric administration, while NS-treated rats given the same volume of NS. Elevated plus maze (EPM) and light/dark transition (LDT) tests were used to observe anti-anxiety effects of RRPPs at 1 h after the last administration. After EPM and LDT tests, hippocampus tissues were excised on ice rapidly from the brains of rats. Thereafter, 2-DE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) were used for detecting differential proteins in hippocampus of rats so as to explore the potential mechanisms.
  
  
  RESULTS
  RRPPs (200, 400 mg/kg) could significantly inhibit MSG-induced decrease of time and entries percentages in open zones in EPM test and numbers of light-dark transition in LDT test. Further analysis of 2-DE and MALDI-TOF/MS indicated that beta-synuclein, protein DJ-1, peroxiredoxin-2, peroxiredoxin-6, dimethylarginine dimethylaminohydrolase 1 (DDAH-1) and iron-sulfur proteins were all found to be down-regulated significantly in MSG-treated rats, while such down-regulation was significantly inhibited after treatment with RRPPs.
  
  
  CONCLUSIONS
  RRPPs showed anti-anxiety effects and potential mechanisms might be related to inhibiting MSG-induced down-regulation of beta-synuclein, DJ-1, peroxiredoxin-2, peroxiredoxin-6, DDAH-1 and iron-sulfur proteins in hippocampus of rats.
authors.affiliation: School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China cyexin@126.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Cui
authors.forename: Chunlei
authors.initials: C
authors.lastname: Rong
authors.forename: Junming
authors.initials: J
authors.lastname: Wang
authors.forename: Can
authors.initials: C
authors.lastname: Cui
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Feng
authors.forename: Jing
authors.initials: J
authors.lastname: Feng
authors.forename: Bing
authors.initials: B
authors.lastname: Niu
mesh_terms: D000818:Animals; D014151:Anti-Anxiety Agents / Q000008:administration & dosage*; D001007:Anxiety / Q000188:drug therapy* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006624:Hippocampus / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008297:Male; D011134:Polysaccharides / Q000008:administration & dosage*; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D032267:Rehmannia / Q000737:chemistry*
chemical_list: D014151:Anti-Anxiety Agents; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D011506:Proteins