title: Protoplast isolation in the marine brown alga Dictyopteris prolifera (Dictyotales).
abstract: Protoplasts were isolated from thalli of Dictyopteris prolifera using a mixture of crude enzymes from vicera of live oysters (Crassostrea gigas) and the following commercial enzymes: an abalone enzyme, cellulase, polygalacturonase and hemicellulase. The enzyme mixtures produced up to 3.3 × 10(7) cells per l g of tissue fresh weight. The conversion to protoplasts of the cells was about 100% using the oyster enzyme or the abalone enzyme alone. The optimum pH for protoplast isolation was 6.0 and 20 hours were required for conversion to protoplasts.
authors.affiliation: Laboratory of Marine Biotechnology, Shiono Koryo Kaisha, Ltd., Niitaka, Yodogawa-ku, 532, Osaka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Fujimura
authors.forename: T
authors.initials: T
authors.lastname: Kawai
authors.forename: T
authors.initials: T
authors.lastname: Kajiwara
authors.forename: Y
authors.initials: Y
authors.lastname: Ishida