title: Establishing high temperature gas chromatographic profiles of non-polar metabolites for quality assessment of African traditional herbal medicinal products.
abstract: The quality assessment of African traditional herbal medicinal products is a difficult challenge since they are complex mixtures of several herbal drug or herbal drug preparations. The plant source is also often unknown and/or highly variable. Plant metabolites chromatographic profiling is therefore an important tool for quality control of such herbal products. The objective of this work is to propose a protocol for sample preparation and gas chromatographic profiling of non-polar metabolites for quality control of African traditional herbal medicinal products. The methodology is based on the chemometric assessment of chromatographic profiles of non-polar metabolites issued from several batches of leaves of Combretum micranthum and Mitracarpus scaber by high temperature gas chromatography coupled to mass spectrometry, performed on extracts obtained in refluxed dichloromethane, after removal of chlorophyll pigments. The method using high temperature gas chromatography after dichloromethane extraction allows detection of most non-polar bioactive and non-bioactive metabolites already identified in leaves of both species. Chemometric data analysis using Principal Component Analysis and Partial Least Squares after Orthogonal Signal Correction applied to chromatographic profiles of leaves of Combretum micranthum and Mitracarpus scaber showed slight batch to batch differences, and allowed clear differentiation of the two herbal extracts.
authors.affiliation: Groupe de Chimie Analytique de Paris-Sud, EA4041 IFR141, Faculté de Pharmacie, Univ. Paris-Sud, 5 rue Jean-Baptiste Clément, 92296 Châtenay-Malabry Cedex, France; Département de Chimie Analytique, Chimie Minérale et Générale, Technologie Alimentaire, Unité de Formation et de Recherche (UFR) des Sciences Pharmaceutiques et Biologiques, Université d'Abidjan-Cocody, 01 BP V 34 Abidjan, Cote d'Ivoire.
authors.forename: Nicaise F
authors.initials: NF
authors.lastname: Bony
authors.forename: Danielle
authors.initials: D
authors.lastname: Libong
authors.forename: Audrey
authors.initials: A
authors.lastname: Solgadi
authors.forename: Jean
authors.initials: J
authors.lastname: Bleton
authors.forename: Pierre
authors.initials: P
authors.lastname: Champy
authors.forename: Anglade K
authors.initials: AK
authors.lastname: Malan
authors.forename: Pierre
authors.initials: P
authors.lastname: Chaminade
mesh_terms: D000349:Africa; D016477:Artifacts; D002623:Chemistry Techniques, Analytical; D002734:Chlorophyll / Q000032:analysis / Q000737:chemistry; D029765:Combretum / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D016018:Least-Squares Analysis; D016488:Medicine, African Traditional / Q000379:methods*; D008752:Methylene Chloride / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D028321:Plant Preparations / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D025341:Principal Component Analysis; D011786:Quality Control; D013696:Temperature
chemical_list: D028321:Plant Preparations; D002734:Chlorophyll; D008752:Methylene Chloride
keywords: African herbal medicinal products; Chemometrics; Combretum micranthum; High temperature gas chromatographic profile; Mitracarpus scaber