title: Screening for a "new" enzyme in nature: Haloperoxidase production by Death Valley dematiaceous hyphomycetes.
abstract: Haloperoxidases are enzymes that have the ability to halogenate a broad range of substrates [10]. To find a biologically produced haloperoxidase that could function at a pH greater than 3.0 and at a temperature greater than 19°C, dematiaceous hyphomycetes were isolated from the Death Valley desert and screened for their ability to produce such an enzyme. A qualitative assay using bromophenol red was employed in situ over a 12-day fermentation period. Several dematiaceous hyphomycetes, such asDreschlera haloides andUlocladium chartarum, produced haloperoxidases that were active in broth culture at 19, 25, and 34°C at pH 7.0 and 8.0.
authors.affiliation: Cetus Corporation, 1400 Fifty-Third Street, 94608, Emeryville, CA, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Hunter-Cevera
authors.forename: L
authors.initials: L
authors.lastname: Sotos