title: The baculovirus sulfhydryl oxidase Ac92 (P33) interacts with the Spodoptera frugiperda P53 protein and oxidizes it in vitro.
abstract: The Autographa californica M nucleopolyhedrovirus (AcMNPV) sulfhydryl oxidase Ac92 is essential for production of infectious virions. Ac92 also interacts with human p53 and enhances human p53-induced apoptosis in insect cells, but it is not known whether any relationship exists between Ac92 and native p53 homologs from insect hosts of AcMNPV. We found that Ac92 interacted with SfP53 from Spodoptera frugiperda in infected cells and oxidized SfP53 in vitro. However, Ac92 did not interact with or oxidize a mutant of SfP53 predicted to lack DNA binding. Silencing Sfp53 expression did not rescue the ability of an ac92-knockout virus to produce infectious virus. Similarly, ac92 expression did not affect SfP53-stimulated caspase activity or the localization of SfP53. Thus, although Ac92 binds to SfP53 during AcMNPV replication and oxidizes SfP53 in vitro, we could not detect any effects of this interaction on AcMNPV replication in cultured cells.
authors.affiliation: Molecular, Cellular, and Developmental Biology Program, Division of Biology, Kansas State University, Manhattan, KS 66506-4901, USA.
authors.forename: Wenbi
authors.initials: W
authors.lastname: Wu
authors.forename: Rollie J
authors.initials: RJ
authors.lastname: Clem
authors.forename: George F
authors.initials: GF
authors.lastname: Rohrmann
authors.forename: A Lorena
authors.initials: AL
authors.lastname: Passarelli
mesh_terms: D000818:Animals; D054884:Host-Pathogen Interactions; D017924:Nucleopolyhedroviruses / Q000201:enzymology* / Q000502:physiology; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D061987:Sf9 Cells; D018411:Spodoptera; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D050862:Oxidoreductases Acting on Sulfur Group Donors
chemical_list: D010088:Oxidoreductases; D016159:Tumor Suppressor Protein p53; D014764:Viral Proteins; D050862:Oxidoreductases Acting on Sulfur Group Donors; C117170:p33 protein, baculovirus
keywords: Ac92; AcMPNV; Baculovirus; Sulfhydryl oxidase; p53