title: Association of apolipoprotein A1 -75 G/A polymorphism with susceptibility to the development of acute lung injury after cardiopulmonary bypass surgery.
abstract: BACKGROUND
  Apolipoprotein A1 (apoA1) is the major apoprotein constituent of high density lipoprotein (HDL) which exerts innate protective effects in systemic inflammation. However, its role in the acute lung injury (ALI) or acute respiratory distress syndrome (ARDS) has not been well studied. The objective of this study was to investigate the potential association between APOA1 -75 G/A polymorphism and the development of ALI after cardiopulmonary bypass (CPB) surgery.
  
  
  METHODS
  A hospital-based case-control study was conducted in patients with ALI (n = 300), patients without ALI (n = 300) and healthy controls (n = 300). Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay was applied to assess the APOA1 -75 G/A genotypes.
  
  
  RESULTS
  Patients with ALI had a significantly higher frequency of APOA1 -75 AA genotype [odds ratio (OR) =1.75, 95% confidence interval (CI) = 1.04, 2.92; P = 0.03] than patients without ALI. APOA1 -75 AA genotype (OR =3.47, 95% CI = 1.60, 7.52; P = 0.002) and A allele (OR =1.92, 95% CI = 1.24, 2.96; P = 0.003) were the significant independent prognostic factors for the 30-day survival rate of patients with ALI after CPB surgery.
  
  
  CONCLUSIONS
  Our study suggested that APOA1 -75 AA genotype was associated with a higher ALI risk after CPB surgery. Patients with the APOA1 -75 AA genotype and A allele had higher 30-day mortality of ALI after CPB surgery. Additional studies are needed to confirm this finding.
authors.forename: Jie
authors.initials: J
authors.lastname: Tu
authors.affiliation: Institute of cardiovascular Diseases, The First Affiliated Hospital of Guangxi, Medical University, 6 ShuangYong Road, Nanning, Guangxi 530021, China. bingdongzhang@live.cn.
authors.forename: Bingdong
authors.initials: B
authors.lastname: Zhang
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Chen
authors.forename: Beiwei
authors.initials: B
authors.lastname: Liang
authors.forename: Dongke
authors.initials: D
authors.lastname: Liang
authors.forename: Guofeng
authors.initials: G
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: He
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D000368:Aged; D000483:Alleles; D016632:Apolipoprotein A-I / Q000235:genetics*; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012306:Risk; D016019:Survival Analysis
chemical_list: C505693:APOA1 protein, human; D016632:Apolipoprotein A-I