title: Binding of puromycin to E. coli ribosomes. Effects of puromycin analogues and peptide bond formation inhibitors.
abstract: (3)H-puromycin binds to bacterial ribosomes, in the presence of ethanol, under the experimental conditions of the fragment reaction assay. The binding is feeble, takes place at 0°C, is partially inhibited by chloramphenicol and lincomycin and totally by sparsomycin. (3)H-puromycin binding is hardly affected by the 3' aminonucleoside of puromycin, is well inhibited by the L-Phenylalanine and L-Leucine analogues of puromycin and totally blocked by L-Phenylalanyl-adenosine.
authors.affiliation: Instituto de Biología Celular, Madrid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Fernandez-Muñoz
authors.forename: D
authors.initials: D
authors.lastname: Vazquez