title: Variations of energy metabolism and adenosine triphosphatase activity in gastric mucosa in chronic atrophic gastritis rats with Qi deficiency and blood stasis syndrome and effect of zhiweifangbian capsule.
abstract: OBJECTIVE
  To study the effects of Zhiweifangbian (ZWFB) capsule on lactic dehydrogenase (LDH), succinic dehydrogenase (SDH) and ATPase activities in gastric mucosa of chronic atrophic gastritis (CAG) rats with Qi deficiency and blood stasis syndrome.
  
  
  METHODS
  Totally 90 rats were randomly divided into 2 groups: normal group (n = 10) and model group (n = 80). The CAG rat model of Qi deficiency and blood stasis type was induced by synthetic methods. After modeling for 12 weeks and the successful CAG model was determined, the CAG model rats were divided by random number table into model group (MG), ZWFB high-dose group (ZWFBH), ZWFB middle-dose group (ZWFBM), ZWFB low-dose group (ZWFBL) and Weimeisu group (WM), 9 rats in each group. The rats in the normal and model groups were intragastrically administrated with distilled water, 10 mL/kg every day; the ZWFB high-dose group with ZWFB, 0.6 g/ kg(-1) x d(-1); the ZWFB middle-dose group with ZWFB, 0.3 g/kg(-1) x d(-1); the ZWFB low-dose group with ZWFB, 0.15 g/kg(-1) x d(-1), and the WM group with suspension of WM, 0.25 g/kg(-1) x d(-1). The treatment was given for 90 consecutive days. Then general survival states were observed and the activities of LDH, SDH, Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase in gastric mucosa tissue were detected.
  
  
  RESULTS
  Compared with the normal group, activity of LDH in the gastric mucosa (P < 0.05) and activities of SDH, Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase significantly decreased in the normal group (P < 0.05). Compared with the model group the activity of LDH decreased and activities of SDH, Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase significantly increased in the high dose ZWFB group (P < 0.05).
  
  
  CONCLUSIONS
  ZWFB capsule can promote energy metabolism and ATPase activity in the gastric mucosa cell, so as to protect the function of the gastric mucosa cell.
authors.affiliation: Institute of Integrated Chinese and Western Medicine, School of Basic Medical Sciences, Lanzhou University, Gansu 730020, China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Duan
authors.forename: Weidong
authors.initials: W
authors.lastname: Cheng
authors.forename: Yingxia
authors.initials: Y
authors.lastname: Cheng
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.forename: Xueyan
authors.initials: X
authors.lastname: Li
authors.forename: Liming
authors.initials: L
authors.lastname: Zhu
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Yang
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D004734:Energy Metabolism / Q000187:drug effects*; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D005757:Gastritis, Atrophic / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D019413:Qi; D051381:Rats; D017208:Rats, Wistar; D013385:Succinate Dehydrogenase / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase; D000251:Adenosine Triphosphatases