title: [Anti-tumor efficacy of P53 with 9R cell-penetrating peptides].
abstract: To enhance the penetration of P53 into tumor cells by fusion it with the cell penetrating peptide 9R. The fusion gene of 9R-p53 was cloned into the expression vector. The fusion protein, CPPs-P53, was expressed and purified. We detected the rate of cell growth inhibition and apoptosis by MTT and Annexin-V-FITC/PI double stained method respectively for measuring its effect on tumor cells. CPPs-P53 and P53 were successfully expressed and purified, the purity of both proteins reached up to 90%. MTT assay showed that the cell growth inhibition by CPPs-P53 was more efficient than P53, and the rate of cell growth inhibition is dose-dependent. The apoptosis experiment showed that P53 could induce apoptosis of tumor cells. Compared with the P53, CPPs-P53 had a more significant effect in inducing cell apoptosis (**P < 0.01). The CPPs-P53 shows more significant effects than P53 in inhibiting cell growth and inducing apoptosis on tumor cells.
authors.affiliation: Biopharmaceutical Teaching and Research Section, College of Life Science, Northeast Agricultural University, Harbin 150030, Heilongjiang, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.forename: Xianlong
authors.initials: X
authors.lastname: Ye
authors.forename: Yin
authors.initials: Y
authors.lastname: Bai
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Wei
authors.forename: Guiping
authors.initials: G
authors.lastname: Ren
authors.forename: Deshan
authors.initials: D
authors.lastname: Li
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D057846:Cell-Penetrating Peptides / Q000494:pharmacology*; D006801:Humans; D016159:Tumor Suppressor Protein p53 / Q000494:pharmacology*
chemical_list: D057846:Cell-Penetrating Peptides; D016159:Tumor Suppressor Protein p53