title: ADAR regulates RNA editing, transcript stability, and gene expression.
abstract: Adenosine deaminases acting on RNA (ADARs) convert adenosine to inosine, which is then recognized as guanosine. To study the role of ADAR proteins in RNA editing and gene regulation, we sequenced and compared the DNA and RNA of human B cells. Then, we followed up the findings experimentally with siRNA knockdown and RNA and protein immunoprecipitations. The results uncovered over 60,000 A-to-G editing sites and several thousand genes whose expression levels are influenced by ADARs. Of these ADAR targets, 90% were identified. Our results also reveal that ADAR regulates transcript stability and gene expression through interaction with HuR (ELAVL1). These findings extend the role of ADAR and show that it cooperates with other RNA-processing proteins to regulate the sequence and expression of transcripts in human cells.
authors.affiliation: Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA. Electronic address: ixwang@umich.edu.
authors.forename: Isabel X
authors.initials: IX
authors.lastname: Wang
authors.forename: Elizabeth
authors.initials: E
authors.lastname: So
authors.forename: James L
authors.initials: JL
authors.lastname: Devlin
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.forename: Ming
authors.initials: M
authors.lastname: Wu
authors.forename: Vivian G
authors.initials: VG
authors.lastname: Cheung
mesh_terms: D000243:Adenosine Deaminase / Q000235:genetics* / Q000378:metabolism*; D001402:B-Lymphocytes / Q000502:physiology; D051959:ELAV Proteins / Q000235:genetics / Q000378:metabolism; D000067780:ELAV-Like Protein 1; D005786:Gene Expression Regulation; D006801:Humans; D012313:RNA / Q000235:genetics* / Q000378:metabolism*; D017393:RNA Editing / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins; D014162:Transfection
chemical_list: D051959:ELAV Proteins; D000067780:ELAV-Like Protein 1; C098770:ELAVL1 protein, human; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012313:RNA; C567955:ADARB1 protein, human; D000243:Adenosine Deaminase