title: Imaging of non-tumorous and tumorous human brain tissues with full-field optical coherence tomography.
abstract: A prospective study was performed on neurosurgical samples from 18 patients to evaluate the use of full-field optical coherence tomography (FF-OCT) in brain tumor diagnosis. FF-OCT captures en face slices of tissue samples at 1 μm resolution in 3D to a penetration depth of around 200 μm. A 1 cm(2) specimen is scanned at a single depth and processed in about 5 min. This rapid imaging process is non-invasive and requires neither contrast agent injection nor tissue preparation, which makes it particularly well suited to medical imaging applications. Temporal chronic epileptic parenchyma and brain tumors such as meningiomas, low-grade and high-grade gliomas, and choroid plexus papilloma were imaged. A subpopulation of neurons, myelin fibers and CNS vasculature were clearly identified. Cortex could be discriminated from white matter, but individual glial cells such as astrocytes (normal or reactive) or oligodendrocytes were not observable. This study reports for the first time on the feasibility of using FF-OCT in a real-time manner as a label-free non-invasive imaging technique in an intraoperative neurosurgical clinical setting to assess tumorous glial and epileptic margins.
authors.affiliation: Inserm U979 "Wave Physics For Medicine" ESPCI -ParisTech - Institut Langevin, 1 rue Jussieu, 75005, France.
authors.forename: Osnath
authors.initials: O
authors.lastname: Assayag
authors.forename: Kate
authors.initials: K
authors.lastname: Grieve
authors.forename: Bertrand
authors.initials: B
authors.lastname: Devaux
authors.forename: Fabrice
authors.initials: F
authors.lastname: Harms
authors.forename: Johan
authors.initials: J
authors.lastname: Pallud
authors.forename: Fabrice
authors.initials: F
authors.lastname: Chretien
authors.forename: Claude
authors.initials: C
authors.lastname: Boccara
authors.forename: Pascale
authors.initials: P
authors.lastname: Varlet
keywords: Brain imaging; Brain tumor; Digital pathology; FF-OCT, full field optical coherence tomography; Glioma; OCT, optical coherence tomography; Optical imaging