title: [Expressions and correlations of let-7a and IL-6 in esophageal squamous cell carcinoma].
abstract: OBJECTIVE
  To investigate the expressions of let-7a and IL-6 in esophageal squamous cell carcinoma (ESCC) tissues and to analyze their correlations.
  
  
  METHODS
  The expression of let-7a in tumor tissues and matched adjacent non-tumor tissues of 40 ESCC patients was detected by stem-loop RT-PCR, and the expression of IL-6 was detected by quantitative real-time PCR (qRT-PCR) and immunohistochemical staining (SP method), respectively.
  
  
  RESULTS
  Let-7a was underexpressed in ESCC tissues compared with matched non-tumor tissues (P<0.01); IL-6 mRNA was overexpressed in ESCC tissues compared with matched non-tumor tissues (P<0.01); the positive rate of IL-6 protein in ESCC tissues was significantly higher than that in non-tumor tissues (P<0.01). There was a negative relationship between the level of let-7a and IL-6 mRNA (r=-0.974; P<0.01), and the level of let-7a in IL-6 positive expression group was lower than that in IL-6 negative expression group (P<0.05).
  
  
  CONCLUSIONS
  Let-7a is down-regulated and IL-6 is overexpressed in ESCC patients, suggesting that there is a significantly negative correlation between them.
authors.affiliation: Institute of Oncology, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang 212001, China.
authors.forename: Deyu
authors.initials: D
authors.lastname: Chen
authors.forename: Liyan
authors.initials: L
authors.lastname: Jin
authors.forename: Longping
authors.initials: L
authors.lastname: Zhu
authors.forename: Xiao
authors.initials: X
authors.lastname: Mou
authors.forename: Shengjun
authors.initials: S
authors.lastname: Wang
authors.forename: Chaoming
authors.initials: C
authors.lastname: Mao
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D035683:MicroRNAs; D012333:RNA, Messenger; C487931:mirnlet7 microRNA, human