title: A novel carboxymethyl chitosan-quantum dot-based intracellular probe for Zn2+ ion sensing in prostate cancer cells.
abstract: In this paper, we fabricated novel carboxymethyl chitosan-coated CdTe quantum dots (CMC-CdTe QDs) via the electrostatic interaction between amino groups in the carboxymethyl chitosan polymeric chains and carboxyl groups of the CdTe QDs. Carboxymethyl chitosan on the surface of CdTe QDs had strong binding ability with Zn(2+), resulting in the obvious enhancement of the photoluminescence of CdTe QDs. The photoluminescence intensity of CMC-CdTe QDs probe was proportional to the concentration of Zn(2+) in the range of 5.0 × 10(-6) to 5.0 × 10(-3) mol l(-1). The detection limit for Zn(2+) was 4.5 × 10(-6) mol l(-1). The experimental results indicate that the CMC-CdTe QDs possess favorable cell compatibility, good sensitivity and selectivity for intracellular Zn(2+) sensing, and are promising candidates for cellular imaging and sensing in prostate cancer cells. The present study also provides an approach for the further development of nanoprobes dedicated to intracellular sensing.
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Zi-Han
authors.initials: ZH
authors.lastname: Lin
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Na
authors.initials: N
authors.lastname: Yang
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China. Electronic address: suxg@jlu.edu.cn.
authors.forename: Xing-Guang
authors.initials: XG
authors.lastname: Su
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D005453:Fluorescence; D006801:Humans; D042541:Intracellular Space; D007477:Ions / Q000378:metabolism; D008297:Male; D015335:Molecular Probes / Q000737:chemistry*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D045663:Quantum Dots / Q000737:chemistry*; D013691:Tellurium / Q000737:chemistry; D013997:Time Factors; D015032:Zinc / Q000378:metabolism*
chemical_list: D019187:Cadmium Compounds; D007477:Ions; D015335:Molecular Probes; C514968:carboxymethyl-chitosan; D048271:Chitosan; D015032:Zinc; D013691:Tellurium; C028337:cadmium telluride
keywords: Intracellular sensing; Prostate cancer cell; Quantum dots; Zn(2+) ion