title: Use of RAPD markers to determine the genetic diversity of diploid, wheat genotypes.
abstract: The genetic diversity of two diploid wheat species, Triticum monococcum and Triticum urartu (2n=2x=14), was assessed using random primers and the polymerase chain reaction (PCR). Electrophoretic analysis of the amplification products revealed a higher incidence of polymorphism in T. urartu than T. monococcum. Pair-wise comparisons of unique and shared polymorphic amplification products, were used to generate Jaccard's similarity coefficients. These were employed to construct phenograms using an unweighted pair-group method with arithmetical averages (UPGMA). The UPGMA analysis indicated a higher similarity among T. monococcum than T. urartu. Analysis of RAPD data appears to be helpful in determining the genetic relationships among genotypes.
authors.affiliation: Plant Genetics Laboratory, Department of Agronomy, Horticulture and Entomology, Texas Tech University, 79409, Lubbock, Texas, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Vierling
authors.forename: H T
authors.initials: HT
authors.lastname: Nguyen