title: Intervention effect and dose-dependent response of tanreqing injection on airway inflammation in lipopolysaccharide-induced rats.
abstract: OBJECTIVE
  To assess the effect of Tanreqing injection on airway inflammation in rats.
  
  
  METHODS
  A rat model of airway inflammation was generated with lipopolysaccharide (LPS). Tanreqing injection was given by intratracheal instillation, and bronchoalveolar lavage fluid (BALF) from the right lung was collected. BALF total cell and neutrophil counts were then determined. In addition, BALF levels of inflammatory cytokines interleukin-13, cytokine-induced neutrophil chemoat-tractant-1, and tumor necrosis factor-alpha were measured using enzyme linked immunosorbent assay. The middle lobe of the right lung was stained with hematoxylin-eosin and histological changes examined.
  
  
  RESULTS
  LPS increased airway inflammation, decreased BALF inflammatory cell count, inflammatory cytokine levels, and suppressed leukocyte influx of the lung. The LPS-induced airway inflammation peaked at 24 h, decreased beginning at 48 h, and had decreased markedly by 96 h.
  
  
  CONCLUSIONS
  Tanreqing injection contains anti-inflammatory properties, and inhibits airway inflammation in a dose-dependent manner.
authors.affiliation: Pneumology Group, Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, West China School of Medicine, Sichuan University, Chengdu 610041, China. wuming-shoujin@sina.com
authors.forename: Shoujin
authors.initials: S
authors.lastname: Dong
authors.forename: Yunqing
authors.initials: Y
authors.lastname: Zhong
authors.forename: Kun
authors.initials: K
authors.lastname: Yang
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Xiong
authors.forename: Bing
authors.initials: B
authors.lastname: Mao
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics / Q000276:immunology; D008070:Lipopolysaccharides / Q000009:adverse effects* / Q000276:immunology; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012140:Respiratory Tract Diseases / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology*
chemical_list: D004365:Drugs, Chinese Herbal; D053583:Interleukin-1beta; D008070:Lipopolysaccharides