title: Inhibitory effect of methyl methanethiosulfinate on β-glucuronidase activity.
abstract: Minced or cut-up leaves of Chinese chive (Allium tuberosum Rottler) contain thiosulfinates and their disproportionate reaction products. Among these organosulfur compounds, methyl methanethiosulfinate was found to be an uncompetitive inhibitor of β-glucuronidase. Approximately 80% of the enzyme activity was inhibited by methyl methanethiosulfinate at 50 µM, the IC50 value being comparable to 3.6 µM.
authors.affiliation: United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Ryoko
authors.initials: R
authors.lastname: Muroi
authors.forename: Haruna
authors.initials: H
authors.lastname: Tsuchiya
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Uda
authors.forename: Kei
authors.initials: K
authors.lastname: Hashimoto
mesh_terms: D000498:Allyl Compounds / Q000737:chemistry; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000737:chemistry; D027782:Chive / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D057075:Enzyme Assays; D005966:Glucuronidase / Q000037:antagonists & inhibitors* / Q000737:chemistry; D007700:Kinetics; D018515:Plant Leaves / Q000737:chemistry*; D012996:Solutions; D013441:Sulfinic Acids / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D000498:Allyl Compounds; D001426:Bacterial Proteins; D004220:Disulfides; D012996:Solutions; D013441:Sulfinic Acids; C082399:methyl methanethiosulfinate; C021181:dimethyl disulfide; C028009:diallyl disulfide; D005966:Glucuronidase