title: [Recognition of excretory/secretory antigens of Anisakis type I and evolution of IgE in experimentally infected rats].
abstract: BACKGROUND
  Anisakis spp., during parasitism, release excretory-secretory antigens that, in contact with the human immune system, can trigger a hypersensitivity response mediated by IgE, causing various allergic symptoms.
  
  
  OBJECTIVE
  To evaluate the IgE response in Wistar rats after infection with L3 larvae of the parasite Anisakis spp.
  
  
  METHODS
  Some determining factors involved in the technique have been improved in this work, such as: the concentration of polyacrylamide used in the preparation of the gels, the antigen concentration used, and the temperature required for denaturation of proteins.
  
  
  RESULTS
  Immune responses (Ag-Ab) observed by the immunoblotting technique showed a greater intensity with serum obtained after reinfection, which have recognized proteins that may correspond to the major antigen Ani s 1 and other polypeptides of interest in the diagnosis of human anisakiasis.
  
  
  CONCLUSIONS
  This paper concludes that immunoblotting is a useful technique to detect IgE antibodies against Anisakis proteins.
authors.affiliation: Departamento de Parasitología, Facultad de Farmacia, Universidad de Granada, Granada, España.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Gómez-Mateos
authors.affiliation: Departamento de Parasitología, Facultad de Farmacia, Universidad de Granada, Granada, España. Electronic address: avalero@ugr.es.
authors.forename: Adela
authors.initials: A
authors.lastname: Valero-López
authors.affiliation: Departamento de Microbiología, Facultad de Farmacia, Universidad de Granada, Granada, España.
authors.forename: Teresa
authors.initials: T
authors.lastname: de la Rubia-Nieto
authors.affiliation: Departamento de Parasitología, Facultad de Farmacia, Universidad de Granada, Granada, España.
authors.forename: María Del Carmen
authors.initials: Mdel C
authors.lastname: Romero-López
authors.affiliation: Departamento de Parasitología, Facultad de Farmacia, Universidad de Granada, Granada, España.
authors.forename: Victoriano
authors.initials: V
authors.lastname: Díaz-Sáez
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D017129:Anisakiasis / Q000276:immunology*; D017163:Anisakis / Q000276:immunology*; D000909:Antibodies, Helminth / Q000096:biosynthesis / Q000097:blood / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology*; D002135:Calcium-Binding Proteins / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D015801:Helminth Proteins / Q000276:immunology*; D007122:Immunoelectrophoresis; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000276:immunology*; D007814:Larva; D011489:Protein Denaturation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000485:Allergens; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D002135:Calcium-Binding Proteins; D015801:Helminth Proteins; C412511:troponin-like protein, Anisakis simplex; D007073:Immunoglobulin E
keywords: Anisakiasis; Anisakiosis; Anisakis simplex spp.; Immunoblotting; Inmunotransferencia