title: Osteogenic activity of nanonized pearl powder/poly (lactide-co-glycolide) composite scaffolds for bone tissue engineering.
abstract: Numerous materials have been proposed for bone tissue engineering. In this study, a newly designed hybrid composite scaffold composed of poly (D,L-lactide-co-glycolide) and a naturally bioceramic hybrid material, nanonized pearl powder, were prepared and the biological activities and physical properties of the scaffold for bone tissue engineering were evaluated. It is a composite consisting calcium carbonate crystal in an aragonite structure, embedded in an organic matrix. Peral contains one or more signal molecules capable of stimulating bone formation. The nanonized pearl powder is considered as a promising osteoinductive biomaterial. This biomaterial is biocompatible and shows osteogenic activity. In this study, the designed biohybrid of nanonized pearl powder/poly (lactide-co-glycolide) (NPP/PLGA) biocomposite scaffolds would employ biodegradable material as MC3T3-E1 cells seeded scaffolds. Therefore, the biocomposite scaffolds would be used to culture with MC3T3-E1 cells under spinner bioreactor in vitro. Furthermore, it also detailed how these tissues were characterized, qualitatively and quantitatively, with scanning electron microscopy and biochemical testing. The identity and the mode of action of these molecules on the osteoblast differentiation were analyzed. This study indicates that the efficiency of nanonized pearl powders in bone cell differentiation are certainly different from that of proteins. Further sudy will look forward to manufacturing the promising new generation bone substitute, three dimensional biocomposite scaffolds to replace the implant and autogeneous bone graft, which combines basic research and clinical application.
authors.affiliation: Department of Traditional Chinese Medicine, Chang Gung Memorial Hospital, Keelung, Taiwan.
authors.forename: Yueh-Lung
authors.initials: YL
authors.lastname: Yang
authors.forename: Ching-Hsien
authors.initials: CH
authors.lastname: Chang
authors.forename: Ching-Cheng
authors.initials: CC
authors.lastname: Huang
authors.forename: Wenny Mei-Wen
authors.initials: WM
authors.lastname: Kao
authors.forename: Wei-Chung
authors.initials: WC
authors.lastname: Liu
authors.forename: Hsia-Wei
authors.initials: HW
authors.lastname: Liu
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D019149:Bioreactors; D001861:Bone Regeneration; D002119:Calcium Carbonate / Q000737:chemistry; D002454:Cell Differentiation; D024042:Collagen Type I / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry; D051379:Mice; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D015675:Osteocalcin / Q000737:chemistry; D010012:Osteogenesis; D053495:Osteopontin / Q000737:chemistry; D011098:Polyglactin 910 / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011208:Powders; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D011208:Powders; D015675:Osteocalcin; D053495:Osteopontin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011098:Polyglactin 910; D002119:Calcium Carbonate
keywords: bio-mineralization; bone tissue engineering; nanonized pearl powder; osteoblast; poly (D, L-lactide-co-glycolide)