title: SPION primes THP1 derived M2 macrophages towards M1-like macrophages.
abstract: Potentially, cellular iron regulates functional plasticity in macrophages yet; interaction of functionally polarized macrophages with iron-oxide nanoparticles has never been studied. We found that monocyte differentiation alters cellular ferritin and cathepsin L levels and induces functional polarization in macrophages. Iron in super paramagnetic iron-oxide nanoparticle (SPION) induces a phenotypic shift in THP1 derived M2 macrophages towards a high CD86+ and high TNF α+ macrophage subtype. This phenotypic shift was accompanied by up-regulated intracellular levels of ferritin and cathepsin L in M2 macrophages, which is a characteristic hallmark of M1 macrophages. Atherogenic oxysterols reduce phagocytic activity in macrophage subtypes, and thus these cells may escape detection by iron-oxide nanoparticles (INPs) in-vivo.
authors.affiliation: Experimental Pathology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping 58185, Sweden; Division of Occupational and Environmental Medicine, University Hospital, Linköping 58185, Sweden. Electronic address: amit.laskar@liu.se.
authors.forename: Amit
authors.initials: A
authors.lastname: Laskar
authors.forename: Jonas
authors.initials: J
authors.lastname: Eilertsen
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Xi-Ming
authors.initials: XM
authors.lastname: Yuan
mesh_terms: D051940:B7-2 Antigen / Q000032:analysis; D056668:Cathepsin L / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D016764:Cell Polarity / Q000187:drug effects*; D002478:Cells, Cultured; D003911:Dextrans / Q000494:pharmacology*; D005290:Ferric Compounds / Q000494:pharmacology; D005293:Ferritins / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007249:Inflammation / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D058185:Magnetite Nanoparticles; D010587:Phagocytosis / Q000187:drug effects; D058226:Plaque, Atherosclerotic / Q000378:metabolism; D013261:Sterols / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D015854:Up-Regulation
chemical_list: D051940:B7-2 Antigen; D003911:Dextrans; D005290:Ferric Compounds; D007074:Immunoglobulin G; D058185:Magnetite Nanoparticles; D013261:Sterols; D014409:Tumor Necrosis Factor-alpha; C000499:ferric oxide; C016658:phorbolol myristate acetate; D005293:Ferritins; D056668:Cathepsin L; C065507:ferumoxides; D013755:Tetradecanoylphorbol Acetate
keywords: Cathepsin L; Ferritin; Iron-oxide nanoparticles; M1 and M2 macrophages; Oxysterols