title: Thin layer chromatography characterization of ELISA specific glycolipids antigens immunomagnetically purified from environmental mycobacteria.
abstract: Thin layer chromatography (TLC) could easily and rapidly evidentiate the qualitative differences between glycolipids (GLs). Different immunomagnetically purified mycobacterial GLs have been compared using TLC, in order to choose the most appropriate antigens to be utilized in ELISA. The GLs were purified from environmental mycobacteria (EM) (M. avium-intracellulare, M. kansasii, M. xenopi, M. scrofulaceum and M. gordonae) and from M. tuberculosis H37Rv. BioMag Amine and BioMag Carboxyl terminated superparamagnetic microparticles were utilized in the magnetic separation of glycolipids from mycobacterial species. TLC of GLs before and after magnetic purification, corroborated with ELISA results, shows that COOH-terminated particles allow a better purification for M. kansasii, M. xenopi and M. scrofulaceum, while NH2-terminated particles act better on MAI and M. gordonae GLs. The use of GL purified antigens in ELISA could fulfill the criteria of high levels of both sensitivity and specificity of serologic assays in EM diagnosis.
authors.affiliation: Cantacuzino National Institute of Research-Development for Microbiology and Immunology, Mycobacterial Antigens Department, Bucharest, Romania.
authors.forename: Irina
authors.initials: I
authors.lastname: Ulea
authors.forename: Henriette
authors.initials: H
authors.lastname: Stavri
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D002855:Chromatography, Thin Layer / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006017:Glycolipids / Q000737:chemistry*; D018189:Immunomagnetic Separation / Q000379:methods*; D009161:Mycobacterium / Q000737:chemistry*; D009164:Mycobacterium Infections / Q000175:diagnosis; D012680:Sensitivity and Specificity
chemical_list: D000942:Antigens, Bacterial; D006017:Glycolipids