title: Effect of vaccination of cattle with the low virulence Nc-Spain 1H isolate of Neospora caninum against a heterologous challenge in early and mid-gestation.
abstract: Live vaccines have emerged as one of the most potentially cost-effective measures for the control of bovine neosporosis. Previous studies have shown that Nc-Spain 1H is a naturally attenuated isolate of Neospora caninum and that immunisation with live Nc-Spain 1H tachyzoites generated a protective immune response in mice. The aim of this study was to evaluate the safety and efficacy of immunisation in cattle. N. caninum-seronegative heifers were immunised subcutaneously twice with 107 live Nc-Spain 1H tachyzoites prior to artificial insemination. No adverse reactions or negative effects on reproductive parameters were recorded following immunisation. In immunised and non-challenged heifers, no foetal deaths were observed, and none of the calves was congenitally infected. The efficacy against N. caninum-associated foetal death and vertical transmission was determined after challenge with high doses of the Nc-1 isolate at 70 and 135 days of gestation, respectively. After the challenge in early gestation, the immunisation induced a protection of 50% against foetal death. In addition, the microsatellite analysis performed in PCR-positive tissue samples from foetuses that died after challenge infection showed that the profiles corresponded to the challenge isolate Nc-1. A degree of protection against vertical transmission was observed after challenge at mid-gestation; calves from immunised heifers showed significantly lower pre-colostral Neospora-specific antibody titres than calves from the non-immunised/challenge group (P < 0.05). Strong antibody and interferon gamma responses were induced in the immunised heifers. This study indicates that the immunisation before pregnancy with the Nc-Spain 1H vaccine isolate appeared to be safe and reduced the occurrence of N. caninum-associated abortion and vertical transmission in experimentally infected cattle. In light of these encouraging results, the next step for testing this live attenuated candidate should be the assessment of its efficacy and safety in naturally infected cattle.
authors.forename: Silvia
authors.initials: S
authors.lastname: Rojo-Montejo
authors.forename: Esther
authors.initials: E
authors.lastname: Collantes-Fernández
authors.forename: Francisco
authors.initials: F
authors.lastname: Pérez-Zaballos
authors.forename: Sonia
authors.initials: S
authors.lastname: Rodríguez-Marcos
authors.forename: Javier
authors.initials: J
authors.lastname: Blanco-Murcia
authors.forename: Antonio
authors.initials: A
authors.lastname: Rodríguez-Bertos
authors.forename: Antoni
authors.initials: A
authors.lastname: Prenafeta
authors.affiliation: SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Avda, Puerta de Hierro s/n, 28040 Madrid, Spain. luis.ortega@vet.ucm.es.
authors.forename: Luis Miguel
authors.initials: LM
authors.lastname: Ortega-Mora
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control*; D003048:Coccidiosis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D005260:Female; D018445:Infectious Disease Transmission, Vertical / Q000517:prevention & control / Q000662:veterinary*; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D018895:Microsatellite Repeats; D018688:Neospora / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011247:Pregnancy; D016052:Protozoan Vaccines / Q000008:administration & dosage / Q000276:immunology*; D013030:Spain; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D016052:Protozoan Vaccines; D014613:Vaccines, Attenuated; D007371:Interferon-gamma