title: Impact of cold ischemia on mitochondrial function in porcine hearts and blood vessels.
abstract: The effects of cold storage using Custodiol® (Histidine-Tryptophan-Ketoglutarate, HTK) or isotonic saline solution on mitochondrial function in hearts (left and rights ventricles) and various blood vessels of pigs were investigated. Hearts, saphenous veins, internal-mammary-arteries and aortas of male landrace pigs were harvested and exposed to cold ischemia in either saline or Custodiol-HTK solution. Mitochondrial function was measured in situ in permeabilized fibers by high-resolution respirometry. Mitochondrial respiratory capacities (maximal respiration rates) were similar in the right and left ventricle in controls and after 14 h of cold storage were significantly better preserved in Custodiol-HTK than in saline solution. Mitochondrial respiration rates in various blood vessels including aorta, arteries and veins were less than 5% of myocardium rates. In contrast to the pig heart, in some blood vessels, like veins, mitochondrial function remained stable even after 24 h of cold ischemia. HTK-Custodiol protection of mitochondrial function after prolonged cold ischemia was observed in the myocardium but not in blood vessels. HTK-Custodiol solution thus offers significant protection of myocardial mitochondria against cold ischemic injury and can be used as efficient preservation solution in organ transplantation but probably has no benefit for blood vessels preservation. Analysis of mitochondrial function can be used as a valuable approach for the assessment of cold ischemic injury in various tissues including pig heart and various blood vessels.
authors.affiliation: Department of Cardiac Surgery, Medical University of Vienna, Vienna A-1090, Austria. dominik.wiedemann@meduniwien.ac.at.
authors.forename: Dominik
authors.initials: D
authors.lastname: Wiedemann
authors.forename: Thomas
authors.initials: T
authors.lastname: Schachner
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Bonaros
authors.forename: Melissa
authors.initials: M
authors.lastname: Dorn
authors.forename: Martin
authors.initials: M
authors.lastname: Andreas
authors.forename: Alfred
authors.initials: A
authors.lastname: Kocher
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Kuznetsov
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000502:physiology*; D019069:Cell Respiration / Q000502:physiology; D050377:Cold Ischemia; D005947:Glucose / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D006321:Heart / Q000503:physiopathology*; D006639:Histidine / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D008929:Mitochondria, Heart / Q000378:metabolism* / Q000502:physiology; D009206:Myocardium; D011189:Potassium Chloride / Q000494:pharmacology; D011343:Procaine / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013552:Swine
chemical_list: C039984:Bretschneider cardioplegic solution; D007328:Insulin; D008353:Mannitol; D012965:Sodium Chloride; D006639:Histidine; D011343:Procaine; D011189:Potassium Chloride; D005978:Glutathione; D005947:Glucose