title: Anti-inflammatory oleanane triterpenes from Tripterygium wilfordii cell suspension cultures by fungal elicitation.
abstract: Treatment of cell suspension cultures of Tripterygium wilfordii with an autoclaved Botrytis sp. homogenate rapidly increased the synthesis of a family of oleanane and friedelane triterpenes, including the antiinflammatory oleanane triterpene 3β,22α-dihydroxyolean-12-en-29-oic acid. This compound exceeded 30 mg · l(-1) in 13 day elicitations with 12 l bioreactors, in contrast to control levels of less than 5 mg · l(-1). Cell cultures treated with the fungal elicitor provided higher triterpene yields in less time than cultures in a diterpene production medium or whole plants. Elicited production has been developed for commercial application in light of the successful treatment of rheumatoid arthritis with Tripterygium extracts.
authors.affiliation: Department of Chemistry, University of British Columbia, 2036 Main Mall, V6T 1Z1, Vancouver, B.C., Canada.
authors.forename: J P
authors.initials: JP
authors.lastname: Kutney
authors.forename: M D
authors.initials: MD
authors.lastname: Samija
authors.forename: G M
authors.initials: GM
authors.lastname: Hewitt
authors.forename: E C
authors.initials: EC
authors.lastname: Bugante
authors.forename: H
authors.initials: H
authors.lastname: Gu