title: New insight to structure-function relationship of GalNAc mediated primary interaction between insecticidal Cry1Ac toxin and HaALP receptor of Helicoverpa armigera.
abstract: Over the last few decades Cry1Ac toxin has been widely used in controlling the insect attack due to its high specificity towards target insects. The pore-forming toxin undergoes a complex mechanism in the insect midgut involving sequential interaction with specific glycosylated receptors in which terminal GalNAc molecule plays a vital role. Recent studies on Cry toxins interactions with specific receptors revealed the importance of several amino acid residues in domain III of Cry1Ac, namely Q509, N510, R511, Y513 and W545, serve as potential binding sites that surround the putative GalNAc binding pocket and mediate the toxin-receptor interaction. In the present study, alanine substitution mutations were generated in the Cry1Ac domain III region and functional significance of those key residues was monitored by insect bioassay on Helicoverpa armigera larvae. In addition, ligand blot analysis and SPR binding assay was performed to monitor the binding characteristics of Cry1Ac wild type and mutant toxins towards HaALP receptor isolated from Helicoverpa armigera. Mutagenesis data revealed that, alanine substitutions in R511, Y513 and W545 substantially impacted the relative affinity towards HaALP receptor and toxicity toward target insect. Furthermore, in silico study of GalNAc-mediated interaction also confirmed the important roles of these residues. This structural analysis will provide a detail insight for evaluating and engineering new generation Cry toxins to address the problem of change in insect behavioral patterns.
authors.affiliation: Division of Plant Biology, Bose Institute, Kolkata, West Bengal, India.
authors.forename: Anindita
authors.initials: A
authors.lastname: Sengupta
authors.forename: Anindya
authors.initials: A
authors.lastname: Sarkar
authors.forename: Prerna
authors.initials: P
authors.lastname: Priya
authors.forename: Shubhra
authors.initials: S
authors.lastname: Ghosh Dastidar
authors.forename: Sampa
authors.initials: S
authors.lastname: Das
mesh_terms: D000409:Alanine / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics / Q000378:metabolism; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites / Q000235:genetics; D004731:Endotoxins / Q000235:genetics / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000235:genetics / Q000378:metabolism*; D019476:Insect Proteins / Q000378:metabolism*; D007306:Insecticides / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D007915:Lepidoptera / Q000378:metabolism*; D016296:Mutagenesis / Q000235:genetics; D013329:Structure-Activity Relationship; D014118:Toxins, Biological / Q000235:genetics / Q000378:metabolism*
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D019476:Insect Proteins; D007306:Insecticides; D014118:Toxins, Biological; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D000409:Alanine