title: Bone morphogenetic protein-7 (BMP-7) mediates ischemic preconditioning-induced ischemic tolerance via attenuating apoptosis in rat brain.
abstract: A mild cerebral ischemic insult, also known as ischemic preconditioning (IPC), confers transient tolerance to a subsequent ischemic challenge in the brain. This study was conducted to investigate whether bone morphogenetic protein-7 (BMP-7) is involved in neuroprotection elicited by IPC in a rat model of ischemia. Ischemic tolerance was induced in rats by IPC (15 min middle cerebral artery occlusion, MCAO) at 48 h before lethal ischemia (2h MCAO). The present data showed that IPC increased BMP-7 mRNA and protein expression after 24h reperfusion following ischemia in the brain. In rats of ischemia, IPC-induced reduction of cerebral infarct volume and improvement of neuronal morphology were attenuated when BMP-7 was inhibited either by antagonist noggin or short interfering RNA (siRNA) pre-treatment. Besides, cerebral IPC-induced up-regulation of B-cell lymphoma 2 (Bcl-2) and down-regulation of cleaved caspase-3 at 24h after ischemia/reperfusion (I/R) injury were reversed via inhibition of BMP-7. These findings indicate that BMP-7 mediates IPC-induced tolerance to cerebral I/R, probably through inhibition of apoptosis.
authors.affiliation: Department of Neurosurgery, Shengjing Hospital, China Medical University, Shenyang 110004, People's Republic of China. Electronic address: junhong.guan@hotmail.com.
authors.forename: Junhong
authors.initials: J
authors.lastname: Guan
authors.forename: Han
authors.initials: H
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Lv
authors.forename: Duo
authors.initials: D
authors.lastname: Chen
authors.forename: Ye
authors.initials: Y
authors.lastname: Yuan
authors.forename: Shengtao
authors.initials: S
authors.lastname: Qu
mesh_terms: D000818:Animals; D017209:Apoptosis; D055419:Bone Morphogenetic Protein 7 / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D001921:Brain / Q000098:blood supply*; D004195:Disease Models, Animal; D020244:Infarction, Middle Cerebral Artery; D019194:Ischemic Preconditioning; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020521:Stroke / Q000378:metabolism / Q000473:pathology*
chemical_list: C525821:Bmp7 protein, rat; D055419:Bone Morphogenetic Protein 7; D012333:RNA, Messenger; D034741:RNA, Small Interfering
keywords: Apoptosis; BMP-7; Cerebral infarction; Cerebral ischemia/reperfusion; Ischemic preconditioning; Neuroprotection