title: A new single nucleotide polymorphism in the calpastatin (CAST) gene associated with beef tenderness.
abstract: First we looked for putative causative mutations in the CAST and CAPN1 genes associated with meat tenderness and found a total of 31 and 7 polymorphisms, respectively, in the Parda de Montaña and Pirenaica breeds. Tenderness was not affected by mutations in CAPN1. However, three SNPs located at intron 5 (BTA7: g.98533962C>G on UMD 3.0), exon 7 (g.98535683A>G) and intron 12 (g.98545188T>A) of the CAST gene were significantly associated with meat tenderness at 7 days post-mortem in the Parda de Montaña breed. The haplotypes h2 and h5 showed significant associations with meat toughness being consistent with the SNP association results, which showed that the g.98535683A>G SNP in CAST might be the causative mutation of the effect found in this study. This mutation changes the amino acid sequence at position p.Thr182Ala (NM_174003). This amino acid substitution could affect the interacting regions between the calpastatin L-domain and calpain, and then could generate a more stable union between calpain and calpastatin.
authors.affiliation: Unidad de Tecnología en Producción Animal, CITA, 59059 Zaragoza, Spain; ARAID, 50004 Zaragoza, Spain. Electronic address: jhcalvo@aragon.es.
authors.forename: J H
authors.initials: JH
authors.lastname: Calvo
authors.forename: L P
authors.initials: LP
authors.lastname: Iguácel
authors.forename: J K
authors.initials: JK
authors.lastname: Kirinus
authors.forename: M
authors.initials: M
authors.lastname: Serrano
authors.forename: G
authors.initials: G
authors.lastname: Ripoll
authors.forename: I
authors.initials: I
authors.lastname: Casasús
authors.forename: M
authors.initials: M
authors.lastname: Joy
authors.forename: L
authors.initials: L
authors.lastname: Pérez-Velasco
authors.forename: P
authors.initials: P
authors.lastname: Sarto
authors.forename: P
authors.initials: P
authors.lastname: Albertí
authors.forename: M
authors.initials: M
authors.lastname: Blanco
mesh_terms: D019943:Amino Acid Substitution / Q000502:physiology; D000818:Animals; D001947:Breeding; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002154:Calpain / Q000235:genetics; D002417:Cattle / Q000145:classification / Q000235:genetics*; D005091:Exons; D005787:Gene Frequency; D006239:Haplotypes; D007438:Introns; D008460:Meat / Q000032:analysis*; D009154:Mutation; D020641:Polymorphism, Single Nucleotide
chemical_list: D002135:Calcium-Binding Proteins; C033668:calpastatin; D002154:Calpain; C484807:CAPN1 protein, Bos taurus
keywords: CAPN1; CAST; Cattle; SNP; Tenderness