title: Microspore culture of swede (Brassica napus ssp. rapifera) and the effects of fresh and conditioned media.
abstract: Microspore culture was successfully carried out with four cultivars of swede (Brassica napus ssp. rapifera). Embryo yields increased markedly if the culture medium was replaced by fresh medium after a culture period of three days, with more than 200% increase with the highly responding cultivar Gry and more than 800% with the poorly responding cultivar Stenhaug. If the medium was changed at a later time, embryo production declined sharply. Transfer of microspores after 3 days of culture to a medium in which microspores of a different cultivar had been cultured for 3 days had no effect on embryogenesis. Bud size had a large effect on embryo production, particularly with the least responsive cultivar, Stenhaug.
authors.affiliation: Department of Horticulture, Agricultural Univ. of Norway, P.O. Box 5022, N-1432 Ås, Norway.
authors.forename: M
authors.initials: M
authors.lastname: Hansen
authors.forename: K
authors.initials: K
authors.lastname: Svinnset