title: Gene expression of growth factors and growth factor receptors for potential targeted therapy of canine hepatocellular carcinoma.
abstract: The purpose of this study was to evaluate the gene expression of growth factors and growth factor receptors of primary hepatic masses, including hepatocellular carcinoma (HCC) and nodular hyperplasia (NH), in dogs. Quantitative real-time reverse transcriptase-polymerase chain reaction was performed to measure the expression of 18 genes in 18 HCCs, 10 NHs, 11 surrounding non-cancerous liver tissues and 4 healthy control liver tissues. Platelet-derived growth factor-B (PDGF-B), transforming growth factor-α, epidermal growth factor receptor, epidermal growth factor and hepatocyte growth factor were found to be differentially expressed in HCC compared with NH and the surrounding non-cancerous and healthy control liver tissues. PDGF-B is suggested to have the potential to become a valuable ancillary target for the treatment of canine HCC.
authors.affiliation: Laboratories of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Gentoku
authors.initials: G
authors.lastname: Iida
authors.forename: Kazushi
authors.initials: K
authors.lastname: Asano
authors.forename: Mamiko
authors.initials: M
authors.lastname: Seki
authors.forename: Manabu
authors.initials: M
authors.lastname: Sakai
authors.forename: Kenji
authors.initials: K
authors.lastname: Kutara
authors.forename: Kumiko
authors.initials: K
authors.lastname: Ishigaki
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Kagawa
authors.forename: Orie
authors.initials: O
authors.lastname: Yoshida
authors.forename: Kenji
authors.initials: K
authors.lastname: Teshima
authors.forename: Kazuya
authors.initials: K
authors.lastname: Edamura
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Watari
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D017931:DNA Primers / Q000235:genetics; D004283:Dog Diseases / Q000235:genetics* / Q000378:metabolism; D004285:Dogs; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism; D020518:Focal Nodular Hyperplasia / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D020574:Proto-Oncogene Proteins c-sis / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D017978:Receptors, Growth Factor / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016211:Transforming Growth Factor alpha / Q000378:metabolism
chemical_list: D017931:DNA Primers; D036341:Intercellular Signaling Peptides and Proteins; D020574:Proto-Oncogene Proteins c-sis; D017978:Receptors, Growth Factor; D016211:Transforming Growth Factor alpha; D004815:Epidermal Growth Factor; D017228:Hepatocyte Growth Factor; D066246:ErbB Receptors