title: Identification of cadmium-induced Agaricus blazei genes through suppression subtractive hybridization.
abstract: Cadmium (Cd) is one of the most serious environmental pollutants. Filamentous fungi are very promising organisms for controlling and reducing the amount of heavy metals released by human and industrial activities. However, the molecular mechanisms involved in Cd accumulation and tolerance of filamentous fungi are not fully understood. Agaricus blazei Murrill, an edible mushroom with medicinal properties, demonstrates high tolerance for heavy metals, especially Cd. To investigate the molecular mechanisms underlying the response of A. blazei after Cd exposure, we constructed a forward subtractive library that represents cadmium-induced genes in A. blazei under 4 ppm Cd stress for 14 days using suppression subtractive hybridization combined with mirror orientation selection. Differential screening allowed us to identify 39 upregulated genes, 26 of which are involved in metabolism, protein fate, cellular transport, transport facilitation and transport routes, cell rescue, defense and virulence, transcription, and the action of proteins with a binding function, and 13 are encoding hypothetical proteins with unknown functions. Induction of six A. blazei genes after Cd exposure was further confirmed by RT-qPCR. The cDNAs isolated in this study contribute to our understanding of genes involved in the biochemical pathways that participate in the response of filamentous fungi to Cd exposure.
authors.affiliation: Zhejiang Forestry Academy, Zhejiang Provincial Key Laboratory of Forest Food, Hangzhou 310023, China.
authors.forename: Liling
authors.initials: L
authors.lastname: Wang
authors.affiliation: Zhejiang Forestry Academy, Zhejiang Provincial Key Laboratory of Forest Food, Hangzhou 310023, China. Electronic address: lihaibozj@126.com.
authors.forename: Haibo
authors.initials: H
authors.lastname: Li
authors.affiliation: Zhejiang Forestry Academy, Zhejiang Provincial Key Laboratory of Forest Food, Hangzhou 310023, China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Wei
authors.affiliation: Zhejiang Forestry Academy, Zhejiang Provincial Key Laboratory of Forest Food, Hangzhou 310023, China; Zhejiang Academy of Medical Science, Hangzhou 310013, China.
authors.forename: Xueqian
authors.initials: X
authors.lastname: Wu
authors.affiliation: Lishui University, Lishui 323000, China.
authors.forename: Leqin
authors.initials: L
authors.lastname: Ke
mesh_terms: D000364:Agaricus / Q000235:genetics*; D001483:Base Sequence; D002104:Cadmium / Q000633:toxicity*; D017931:DNA Primers; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D005800:Genes, Fungal; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D017931:DNA Primers; D002104:Cadmium
keywords: Agaricus blazei; Cadmium; Edible mushroom; Gene expression; Suppression subtractive hybridization