title: Mallotus philippinensis bark extracts promote preferential migration of mesenchymal stem cells and improve wound healing in mice.
abstract: In the present study, we report the effects of the ethanol extract from Mallotus philippinensis bark (EMPB) on mesenchymal stem cell (MSC) proliferation, migration, and wound healing in vitro and in a mouse model. Chemotaxis assays demonstrated that EMPB acted an MSC chemoattractant and that the main chemotactic activity of EMPB may be due to the effects of cinnamtannin B-1. Flow cytometric analysis of peripheral blood mononuclear cells in EMPB-injected mice indicated that EMPB enhanced the mobilization of endogenous MSCs into blood circulation. Bioluminescent whole-animal imaging of luciferase-expressing MSCs revealed that EMPB augmented the homing of MSCs to wounds. In addition, the efficacy of EMPB on migration of MSCs was higher than that of other skin cell types, and EMPB treatment improved of wound healing in a diabetic mouse model. The histopathological characteristics demonstrated that the effects of EMPB treatment resembled MSC-induced tissue repair. Taken together, these results suggested that EMPB activated the mobilization and homing of MSCs to wounds and that enhancement of MSC migration may improve wound healing.
authors.affiliation: Skin Regeneration, PIAS Collaborative Research, UIC, Osaka University, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Furumoto
authors.affiliation: Skin Regeneration, PIAS Collaborative Research, UIC, Osaka University, Japan; Research & Development Division, PIAS Corporation, Japan.
authors.forename: Noriyasu
authors.initials: N
authors.lastname: Ozawa
authors.affiliation: Skin Regeneration, PIAS Collaborative Research, UIC, Osaka University, Japan; Research & Development Division, PIAS Corporation, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Inami
authors.affiliation: Skin Regeneration, PIAS Collaborative Research, UIC, Osaka University, Japan; Research & Development Division, PIAS Corporation, Japan.
authors.forename: Misaki
authors.initials: M
authors.lastname: Toyoshima
authors.affiliation: Skin Regeneration, PIAS Collaborative Research, UIC, Osaka University, Japan; Research & Development Division, PIAS Corporation, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Fujita
authors.affiliation: Skin Regeneration, PIAS Collaborative Research, UIC, Osaka University, Japan; Research & Development Division, PIAS Corporation, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Zaiki
authors.affiliation: Skin Regeneration, PIAS Collaborative Research, UIC, Osaka University, Japan; Research & Development Division, PIAS Corporation, Japan.
authors.forename: Shunya
authors.initials: S
authors.lastname: Sahara
authors.affiliation: Skin Regeneration, PIAS Collaborative Research, UIC, Osaka University, Japan; Research & Development Division, PIAS Corporation, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Akita
authors.affiliation: Skin Regeneration, PIAS Collaborative Research, UIC, Osaka University, Japan; Research & Development Division, PIAS Corporation, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Kitamura
authors.affiliation: Skin Regeneration, PIAS Collaborative Research, UIC, Osaka University, Japan; Research & Development Division, PIAS Corporation, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Nakaoji
authors.affiliation: Research & Development Division, PIAS Corporation, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Hamada
authors.affiliation: Division of Stem Cell Therapy Science, Graduate School of Medicine, Osaka University, Japan.
authors.forename: Katsuto
authors.initials: K
authors.lastname: Tamai
authors.affiliation: Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Japan.
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Kaneda
authors.affiliation: Skin Regeneration, PIAS Collaborative Research, UIC, Osaka University, Japan. Electronic address: amaeda@uic.osaka-u.ac.jp.
authors.forename: Akito
authors.initials: A
authors.lastname: Maeda
mesh_terms: D000818:Animals; D002630:Chemotactic Factors; D002633:Chemotaxis / Q000187:drug effects*; D048909:Diabetes Complications / Q000188:drug therapy*; D003921:Diabetes Mellitus, Experimental / Q000150:complications; D004195:Disease Models, Animal; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D036461:Mallotus Plant / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D051379:Mice; D008517:Phytotherapy; D024301:Plant Bark; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D044945:Proanthocyanidins / Q000494:pharmacology / Q000627:therapeutic use; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy*
chemical_list: D002630:Chemotactic Factors; D010936:Plant Extracts; D044945:Proanthocyanidins; C517872:cinnamtannin B-1
keywords: Mallotus philippinensis; Mesenchymal stem cells; Migration; Phenolic compounds; Wound healing