title: Cambial tissue culture and subsequent shoot regeneration from mature black locust (Robinia pseudoacacia L.).
abstract: Callus initiation and subsequent shoot regeneration were obtained from cambial tissue of mature black locust trees. Shoot regeneration was highly tree specific, and was only achieved when calli derived from a particular genotype (tree #1) were transferred to medium containing 6-benzylaminopurine. The studies described here show that the immediate use of a mature genotype as starting material for tissue cultural manipulation is possible by using cambial explants with the proper in vitro conditions. We also found that stems could be stored at 4°C for 8 months prior to culture initiation, with no decline in the viability of the cultures. Tissue sources can thus be continuously available.
authors.affiliation: Department of Biochemistry, SJ-70, University of Washington, 98195, Seattle, WA, USA.
authors.forename: K H
authors.initials: KH
authors.lastname: Han
authors.forename: D E
authors.initials: DE
authors.lastname: Keathley
authors.forename: M P
authors.initials: MP
authors.lastname: Gordon