title: ATP and GTP hydrolysis assays (TLC).
abstract: Many biochemical reactions that occur within the cell are thermodynamically unfavorable. However, when these reactions are coupled to NTP (nucleoside triphosphate) hydrolysis, the energy derived from the hydrolysis of the phosphodiester bond helps drive the reaction in the favorable direction. Examples of such proteins can be found in almost all facets of cellular metabolism: glycolysis and the TCA cycle, protein biosynthesis, DNA and RNA metabolism, cellular trafficking, cell signaling, cell division etc. Thus, characterization of the NTPase activity of these proteins in vitro can help in understanding the role of the protein in complex cellular processes.
authors.affiliation: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Vaishnavi
authors.initials: V
authors.lastname: Rajagopal
authors.forename: Jon R
authors.initials: JR
authors.lastname: Lorsch
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis / Q000378:metabolism*; D002855:Chromatography, Thin Layer / Q000295:instrumentation / Q000379:methods*; D006160:Guanosine Triphosphate / Q000032:analysis / Q000378:metabolism*; D006868:Hydrolysis; D017361:Viral Nonstructural Proteins / Q000378:metabolism; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D000255:Adenosine Triphosphate; D006160:Guanosine Triphosphate; D017361:Viral Nonstructural Proteins; C084422:NS3 protein, hepatitis C virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
keywords: Adenosine triphosphate (ATP) hydrolysis; Guanosine triphosphate (ATP) hydrolysis; Nucleoside diphosphate (NDP); Nucleoside triphosphate (NTP); Preparation of the thin-layer chromatography plate and chamber; Thin-layer chromatography (TLC) separation