title: The novel coding region SNPs of PPARGC1A gene and their associations with growth traits in Chinese native cattle.
abstract: The peroxisome proliferator-activated receptor gamma coactivator-1 alpha protein, encoded by the PPARGC1A gene, is a metabolic switch, which transcriptionally activates a complex pathway of mitochondrial biogenesis, lipid and glucose metabolism. Three SNPs (exon 3 c.396G>A, intron 9 c.1892 + 19C>T and exon 10 c.1971C>T) were found and identified in three Chinese native cattle breeds by PCR-SSCP, PCR-RFLP and DNA sequencing methods. All three populations had a low genetic diversity at SNP396 locus (PIC <0.25) while possessed a moderate genetic diversity at SNP1892 locus (0.25 < PIC < 0.5). Association study indicated that the synonymous mutation c.396G>A significantly associated with body weight and average daily gain in Nanyang cattle at the adult age (P < 0.05). Our investigation will not only extend the spectrum of genetic variation of bovine PPARGC1A gene, but also provide useful information for the marker assisted selection in beef cattle breeding program.
authors.affiliation: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, No.22 Xinong Road, Yangling, 712100, Shaanxi, People's Republic of China, mijieli@yahoo.com.cn.
authors.forename: Mijie
authors.initials: M
authors.lastname: Li
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.forename: Xianyong
authors.initials: X
authors.lastname: Lan
authors.forename: Chuzhao
authors.initials: C
authors.lastname: Lei
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D049628:Body Size; D001835:Body Weight; D001947:Breeding; D002417:Cattle / Q000235:genetics* / Q000254:growth & development; D056726:Genetic Association Studies; D005819:Genetic Markers; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D014157:Transcription Factors