title: Jellyfish collagen scaffolds for cartilage tissue engineering.
abstract: Porous scaffolds were engineered from refibrillized collagen of the jellyfish Rhopilema esculentum for potential application in cartilage regeneration. The influence of collagen concentration, salinity and temperature on fibril formation was evaluated by turbidity measurements and quantification of fibrillized collagen. The formation of collagen fibrils with a typical banding pattern was confirmed by atomic force microscopy and transmission electron microscopy analysis. Porous scaffolds from jellyfish collagen, refibrillized under optimized conditions, were fabricated by freeze-drying and subsequent chemical cross-linking. Scaffolds possessed an open porosity of 98.2%. The samples were stable under cyclic compression and displayed an elastic behavior. Cytotoxicity tests with human mesenchymal stem cells (hMSCs) did not reveal any cytotoxic effects of the material. Chondrogenic markers SOX9, collagen II and aggrecan were upregulated in direct cultures of hMSCs upon chondrogenic stimulation. The formation of typical extracellular matrix components was further confirmed by quantification of sulfated glycosaminoglycans.
authors.affiliation: University Hospital and Medical Faculty, Technische Universitaät Dresden, Centre for Translational Bone, Joint and Soft Tissue Research, Fetscher Str. 74, D-01307 Dresden, Germany. Electronic address: birgit.hoyer@tu-dresden.de.
authors.forename: Birgit
authors.initials: B
authors.lastname: Hoyer
authors.affiliation: University Hospital and Medical Faculty, Technische Universitaät Dresden, Centre for Translational Bone, Joint and Soft Tissue Research, Fetscher Str. 74, D-01307 Dresden, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Bernhardt
authors.affiliation: University Hospital and Medical Faculty, Technische Universitaät Dresden, Centre for Translational Bone, Joint and Soft Tissue Research, Fetscher Str. 74, D-01307 Dresden, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Lode
authors.affiliation: Max Bergmann Center of Biomaterials and Institute for Materials Science, Technische Universität Dresden, Budapester Str. 27, 01069 Dresden, Germany.
authors.forename: Sascha
authors.initials: S
authors.lastname: Heinemann
authors.affiliation: Institute of Virology and Cell Biology, University of Lübeck, Ratzeburger Allee 160, 23562 Lübeck, Germany; CRM Coastal Research & Management GmbH, Tiessenkai 12, 24159 Kiel, Germany.
authors.forename: Judith
authors.initials: J
authors.lastname: Sewing
authors.affiliation: Institute of Anatomy, University of Lübeck, Ratzeburger Allee 160, 23562 Lübeck, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Klinger
authors.affiliation: Institute of Virology and Cell Biology, University of Lübeck, Ratzeburger Allee 160, 23562 Lübeck, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Notbohm
authors.affiliation: University Hospital and Medical Faculty, Technische Universitaät Dresden, Centre for Translational Bone, Joint and Soft Tissue Research, Fetscher Str. 74, D-01307 Dresden, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Gelinsky
mesh_terms: D000818:Animals; D002152:Calorimetry, Differential Scanning; D002356:Cartilage / Q000187:drug effects / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020219:Chondrogenesis / Q000187:drug effects; D003063:Cnidaria / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D009994:Osmolar Concentration; D012965:Sodium Chloride / Q000494:pharmacology; D013314:Stress, Mechanical; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D012965:Sodium Chloride; D003094:Collagen
keywords: Chondrogenic differentiation; Fibril formation; Jellyfish; Marine collagen; Mesenchymal stem cells