title: Plant regeneration from protoplasts isolated from long-term cell cultures of wheat (Triticum aestivum L.).
abstract: A friable and fast-growing type of callus was isolated from a long term shoot-competent cell culture of wheat. The suspension cultures established from this callus consisted of small, densely cytoplasmic cells which divided more rapidly but with a lower plant regeneration frequency than the original culture. A high yield of protoplasts was released from suspension cells (2 to 3×10(7) protoplasts per ml packed cell volume) when treated with enzyme mixtures. The isolated protoplasts divided at a relatively high frequency (20% to 50%) in both liquid and agarose-solidified KM8p medium. Up to 0.21% of the dividing protoplasts continued to divide and form micro-calli. Sixty-eight plants were regenerated from micro-calli, and among the 30 plants which were transplanted to the greenhouse, 3 have survived.
authors.affiliation: Sogetal Inc., 3876 Bay Center Place, 94545, Hayward, California, USA.
authors.forename: Y F
authors.initials: YF
authors.lastname: Chang
authors.forename: W C
authors.initials: WC
authors.lastname: Wang
authors.forename: C Y
authors.initials: CY
authors.lastname: Warfield
authors.forename: H T
authors.initials: HT
authors.lastname: Nguyen
authors.forename: J R
authors.initials: JR
authors.lastname: Wong