title: Cleavage of bacteriophage M13 DNA by Haemophilus influenzae endonuclease-R.
abstract: The restriction enzyme from Haemophilus influenzae, endonuclease-R, has only one cleavage site on the double-stranded replicative form DNA of bacteriophage M13. Circular replicative forms are broken to yield full-length liniar M13-DNA molecules (RF-III). The cleavage site appears to be specific as the RF-III molecules, produced by endonuclease-R, cannot be circularized by denaturation and renaturation.
authors.affiliation: Laboratory of Molecular Biology, University of Nijmegen, Nijmegen, The Netherlands.
authors.forename: C M
authors.initials: CM
authors.lastname: Van den Hondel
authors.forename: J G
authors.initials: JG
authors.lastname: Schoenmakers