title: (-)-Epigallocatechin-gallate (EGCG) stabilize the mitochondrial enzymes and inhibits the apoptosis in cigarette smoke-induced myocardial dysfunction in rats.
abstract: The present study brings out the preventive role of (-)-epigallocatechin-gallate (EGCG) on cardiac mitochondrial metabolism and apoptosis in cigarette smoke (CS)-exposed rats. The CS-exposed rats showed significantly decreased activities of TCA cycle enzymes and mitochondrial enzymatic antioxidants, on the other hand, mitochondrial lipid peroxidation was increased and GSH level was decreased. Further, CS exposure was found to induce cardiac apoptosis through release of cytochrome c into the cytosol, cleavage of pro-caspase-3 to active caspase-3, up-regulation of pro-apoptotic (Bax) and down-regulation of antiapoptotic (Bcl-2) molecules. The CS-induced apoptosis was further confirmed by mitochondrial and nuclear ultra structural apoptotic features as evaluated by electron microscopic studies. EGCG supplementation shelters the activities of TCA cycle enzymes and antioxidant enzymes, with concomitant decrease in lipid peroxidation and increase in GSH level. EGCG administration inhibited apoptosis through the inhibition of cytochrome c release into cytosol, activation of pro-caspase-3, down regulation of Bax and significant up regulation of Bcl-2. EGCG reversed the ultra structural apoptotic alterations of mitochondria and nucleus. The present study has provided experimental evidences that the EGCG treatment enduring to cardio protection at mitochondrial level.
authors.affiliation: Department of Biochemistry, Periyar University, Salem, 636016, Tamil Nadu, India.
authors.forename: Gokulakrishnan
authors.initials: G
authors.lastname: Adikesavan
authors.forename: Magendira Mani
authors.initials: MM
authors.lastname: Vinayagam
authors.forename: Liyakath Ali
authors.initials: LA
authors.lastname: Abdulrahman
authors.forename: Thirunavukkarasu
authors.initials: T
authors.lastname: Chinnasamy
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D001835:Body Weight / Q000187:drug effects; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002952:Citric Acid Cycle / Q000187:drug effects; D004587:Electrophoresis, Agar Gel; D004795:Enzyme Stability / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D006321:Heart / Q000187:drug effects / Q000503:physiopathology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D009206:Myocardium / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012907:Smoking / Q000009:adverse effects*
chemical_list: D000975:Antioxidants; D002392:Catechin; C045651:epigallocatechin gallate; D005978:Glutathione