title: Crystallization and preliminary X-ray diffraction analysis of the lactonase VmoLac from Vulcanisaeta moutnovskia.
abstract: Phosphotriesterase-like lactonases (PLLs) are native lactonases that are capable of hydrolyzing lactones such as aliphatic lactones or acyl-homoserine lactones, which are involved in bacterial quorum sensing. Previously characterized PLLs are moreover endowed with a promiscuous phosphotriesterase activity and are therefore able to detoxify organophosphate insecticides. A novel PLL representative, dubbed VmoLac, has been identified from the hyperthermophilic crenarchaeon Vulcanisaeta moutnovskia. Because of its intrinsic high thermal stability, VmoLac may constitute an appealing candidate for engineering studies with the aim of producing an efficient biodecontaminant for organophosphorus compounds and a bacterial antivirulence agent. In combination with biochemical studies, structural information will allow the identification of the residues involved in substrate specificity and an understanding of the enzymatic catalytic mechanisms. Here, the expression, purification, crystallization and X-ray data collection at 2.4 Å resolution of VmoLac are reported.
authors.affiliation: Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergents (URMITE), UMR CNRS-IRD-INSERM, IHU Méditerranée Infection, Aix-Marseille Université, 7 Boulevard Jean Moulin, 13385 Marseille CEDEX 05, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Hiblot
authors.forename: Guillaume
authors.initials: G
authors.lastname: Gotthard
authors.forename: Charlotte
authors.initials: C
authors.lastname: Champion
authors.forename: Eric
authors.initials: E
authors.lastname: Chabriere
authors.forename: Mikael
authors.initials: M
authors.lastname: Elias
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry*; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry*; D019615:Crenarchaeota / Q000201:enzymology*; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D014961:X-Ray Diffraction
chemical_list: D019843:Archaeal Proteins; D002265:Carboxylic Ester Hydrolases
keywords: Vulcanisaeta moutnovskia; extremophiles; lactonases; molecular promiscuity; organophosphorus; phosphotriesterases; quorum sensing