title: Abnormal expression of paxillin correlates with tumor progression and poor survival in patients with gastric cancer.
abstract: BACKGROUND
  Paxillin (PXN) has been found to be aberrantly regulated in various malignancies and involved in tumor growth and invasion. The clinicopathological and prognostic significance of PXN in gastric cancer is still unclear.
  
  
  METHODS
  The expression of PXN was determined in paired gastric cancer tissues and adjacent normal tissues by Western blotting and real-time PCR. Immunohistochemistry was performed to detect the expression of PXN in 239 gastric cancer patients. Statistical analysis was applied to investigate the correlation between PXN expression and clinicopathological characteristics and prognosis in patients. Additionally, the effects of PXN on gastric cancer cell proliferation and migration were also evaluated.
  
  
  RESULTS
  PXN was up-regulated in gastric cancer tissues and cell lines as compared with adjacent normal tissues and normal gastric epithelial cell line GES-1. Overexpression of PXN was correlated with distant metastasis (P=0.001) and advanced tumor stage (P=0.021) in gastric cancer patients. Patients with high PXN expression tended to have poor prognosis compared with patients with low PXN expression (P<0.001). Multivariate analysis demonstrated that PXN expression was an independent prognostic factor (P=0.020). Moreover, ectopic expression of PXN promotes cell proliferation and migration in AGS cells whereas knockdown of PXN inhibits cell proliferation and migration in SGC7901 cells.
  
  
  CONCLUSIONS
  PXN plays an important role in tumor progression and may be used as a potential prognostic indicator in gastric cancer.
authors.forename: Dong-liang
authors.initials: DL
authors.lastname: Chen
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Wang
authors.forename: Chao
authors.initials: C
authors.lastname: Ren
authors.forename: Zhao-lei
authors.initials: ZL
authors.lastname: Zeng
authors.forename: De-shen
authors.initials: DS
authors.lastname: Wang
authors.forename: Hui-yan
authors.initials: HY
authors.lastname: Luo
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Miao-zhen
authors.initials: MZ
authors.lastname: Qiu
authors.forename: Long
authors.initials: L
authors.lastname: Bai
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Zhang
authors.forename: Feng-hua
authors.initials: FH
authors.lastname: Wang
authors.forename: Yu-hong
authors.initials: YH
authors.lastname: Li
authors.affiliation: State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Dong Feng East Road, 510060 Guangzhou, P,R, China. xurh@sysucc.org.cn.
authors.forename: Rui-hua
authors.initials: RH
authors.lastname: Xu
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D017931:DNA Primers; D018450:Disease Progression; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D051419:Paxillin / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D015996:Survival Rate
chemical_list: D017931:DNA Primers; D051419:Paxillin