title: The multifaceted effect of PB1-F2 specific antibodies on influenza A virus infection.
abstract: PB1-F2 is a small influenza A virus (IAV) protein encoded by an alternative reading frame of the PB1 gene. During IAV infection, antibodies to PB1-F2 proteins are induced. To determine their function and contribution to virus infection, three distinct approaches were employed: passive transfer of anti-PB1-F2 MAbs and polyclonal antibodies, active immunization with PB1-F2 peptides and DNA vaccination with plasmids expressing various parts of PB1-F2. Mostly N-terminal specific antibodies were detected in polyclonal sera raised to complete PB1-F2. Passive and active immunization revealed that antibodies recognizing the N-terminal part of the PB1-F2 molecule have no remarkable effect on the course of IAV infection. Interestingly antibodies against the C-terminal region of PB1-F2, obtained by immunization with KLH-PB1-F2 C-terminal peptide or DNA immunization with pC-ter.PB1-F2 plasmid, partially protected mice against virus infection. To our knowledge, this is the first report demonstrating the biological relevance of humoral immunity against PB1-F2 protein in vivo.
authors.affiliation: Institute of Virology, Slovak Academy of Sciences, Dúbravská cesta 9, 845 05 Bratislava, Slovak Republic.
authors.forename: I
authors.initials: I
authors.lastname: Košík
authors.forename: I
authors.initials: I
authors.lastname: Krejnusová
authors.forename: M
authors.initials: M
authors.lastname: Práznovská
authors.forename: G
authors.initials: G
authors.lastname: Russ
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D001835:Body Weight; D005260:Female; D007114:Immunization / Q000379:methods; D007252:Influenza Vaccines / Q000008:administration & dosage; D008168:Lung / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000276:immunology* / Q000473:pathology*; D016019:Survival Analysis; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007252:Influenza Vaccines; C442816:PB1-F2 protein, Influenza A virus; D014764:Viral Proteins
keywords: Antibodies; DNA vaccination; Humoral immunity; Immunogenicity; Influenza; PB1-F2