title: Inactivation of Escherichia coli O157:H7 on stainless steel upon exposure to Paenibacillus polymyxa biofilms.
abstract: We investigated the potential use of biofilm formed by a competitive-exclusion (CE) microorganism to inactivate Escherichia coli O157:H7 on a stainless steel surface. Five microorganisms showing inhibitory activities against E. coli O157:H7 were isolated from vegetable seeds and sprouts. The microorganism with the greatest antimicrobial activity was identified as Paenibacillus polymyxa (strain T5). In tryptic soy broth (TSB), strain T5 reached a higher population at 25 °C than at 12 or 37 °C without losing inhibitory activity against E. coli O157:H7. When P. polymyxa (6 log CFU/mL) was co-cultured with E. coli O157:H7 (2, 3, 4, or 5 log CFU/mL) in TSB at 25 °C, the number of E. coli O157:H7 decreased significantly within 24h. P. polymyxa formed a biofilm on stainless steel coupons (SSCs) in TSB at 25 °C within 24h, and cells in biofilms, compared to attached cells without biofilm formation, showed significantly increased resistance to a dry environment (43% relative humidity [RH]). With the exception of an inoculum of 4 log CFU/coupon at 100% RH, upon exposure to biofilm formed by P. polymyxa on SSCs, populations of E. coli O157:H7 (2, 4, or 6 log CFU/coupon) were significantly reduced within 48 h. Most notably, when E. coli O157:H7 at 2 log CFU/coupon was applied to SSCs on which P. polymyxa biofilm had formed, it was inactivated within 1h, regardless of RH. These results will be useful when developing strategies using biofilms produced by competitive exclusion microorganisms to inactivate foodborne pathogens in food processing environments.
authors.affiliation: Department of Food Bioscience and Technology, Korea University, Anam-dong, Sungbuk-ku, Seoul 136-701, Republic of Korea.
authors.forename: Seonhwa
authors.initials: S
authors.lastname: Kim
authors.forename: Jihyun
authors.initials: J
authors.lastname: Bang
authors.forename: Hoikyung
authors.initials: H
authors.lastname: Kim
authors.forename: Larry R
authors.initials: LR
authors.lastname: Beuchat
authors.forename: Jee-Hoon
authors.initials: JH
authors.lastname: Ryu
mesh_terms: D000898:Antibiosis; D018441:Biofilms; D015169:Colony Count, Microbial; D019453:Escherichia coli O157 / Q000502:physiology*; D005516:Food Microbiology / Q000379:methods*; D050296:Microbial Viability; D056507:Paenibacillus / Q000502:physiology*; D013193:Stainless Steel
chemical_list: D013193:Stainless Steel
keywords: Biofilm; Competitive-exclusion; Escherichia coli O157:H7; Paenibacillus polymyxa; Stainless steel surface