title: Loss of 2 um DNA from Saccharomyces cerevisiae transformed with the chimaeric plasmid pJDB219.
abstract: Two plasmids containing Saccharomyces cerevisiae 2 µm DNA sequences and the S. cerevisiae LEU2 gene have been found to display incompatibility with 2 µm DNA; in the presence of the LEU2 plasmids, 2 µm DNA can be lost. The LEU2 plasmids can be lost spontaneously after (and before) 2 µm DNA loss has occurred, so that strains completely lacking 2 µm DNA sequences can be obtained routinely.
authors.affiliation: Botany School, Sóuth Parks Road, 0X1 3RA, Oxford, England.
authors.forename: M J
authors.initials: MJ
authors.lastname: Dobson
authors.forename: A B
authors.initials: AB
authors.lastname: Futcher
authors.forename: B S
authors.initials: BS
authors.lastname: Cox