title: Pancreatic stellate cells promote hapto-migration of cancer cells through collagen I-mediated signalling pathway.
abstract: BACKGROUND
  Pancreatic stellate cells (PSCs) promote metastasis as well as local growth of pancreatic cancer. However, the factors mediating the effect of PSCs on pancreatic cancer cells have not been clearly identified.
  
  
  METHODS
  We used a modified Boyden chamber assay as an in vitro model to investigate the role of PSCs in migration of Panc1 and UlaPaCa cells and to identify the underlying mechanisms.
  
  
  RESULTS
  PSC supernatant (PSC-SN) dose-dependently induced the trans-migration of Panc1 and UlaPaCa cells, mainly via haptokinesis and haptotaxis, respectively. In contrast to poly-L-lysine or fibronectin, collagen I resembled PSC-SN with respect to its effect on cancer cell behaviours, including polarised morphology, facilitated adhesion, accelerated motility and stimulated trans-migration. Blocking antibodies against integrin α2/β1 subunits significantly attenuated PSC-SN- or collagen I-promoted cell trans-migration and adhesion. Moreover, both PSC-SN and collagen I induced the formation of F-actin and focal adhesions in cells, which was consistent with the constantly enhanced phosphorylation of focal adhesion kinase (FAK, Tyr397). Inhibition of FAK function by an inhibitor or small interference RNAs significantly diminished the effect of PSC-SN or collagen I on haptotaxis/haptokinesis of pancreatic cancer cells.
  
  
  CONCLUSIONS
  Collagen I is the major mediator for PSC-SN-induced haptokinesis of Panc1 and haptotaxis of UlaPaCa by activating FAK signalling via binding to integrin α2β1.
authors.affiliation: Department of Clinical Chemistry, University of Ulm, Albert-Einstein-Allee 23, 89081, Ulm, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Clinical Chemistry, University of Ulm, Albert-Einstein-Allee 23, 89081, Ulm, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, Ostalb Hospital, Im Kälblesrain 1, 73430, Aalen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Siech
authors.affiliation: Department of Clinical Chemistry, University of Ulm, Albert-Einstein-Allee 23, 89081, Ulm, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Habisch
authors.affiliation: Department of Internal Medicine, University of Ulm, Albert-Einstein-Allee 23, 89081, Ulm, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Seufferlein
authors.affiliation: Department of Clinical Chemistry, University of Ulm, Albert-Einstein-Allee 23, 89081, Ulm, Germany.
authors.forename: M G
authors.initials: MG
authors.lastname: Bachem
mesh_terms: D000199:Actins / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D024042:Collagen Type I / Q000378:metabolism*; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000473:pathology*; D010190:Pancreatic Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology*; D058954:Pancreatic Stellate Cells / Q000378:metabolism / Q000473:pathology*; D010766:Phosphorylation / Q000502:physiology; D015398:Signal Transduction
chemical_list: D000199:Actins; D024042:Collagen Type I; D051416:Focal Adhesion Protein-Tyrosine Kinases