title: Characterization of digestive enzyme activity during larval development of gilthead seabream (Sparus aurata).
abstract: The evolution of the digestive enzyme equipment in seabream from hatching to 30 days old larvae was studied; there was a progressive increase in the activity of protease, amylase and acid and alkaline phosphatase from day 15 onwards. The use of specific inhibitors, and SDS-PAGE provided evidence to suggest that most of the proteases belonged to the serine group. A high α-amylase activity was also denoted. Zymograms of larval extracts indicated that exogenous food has more a qualitative than a quantitative role in the secretion of digestive enzymes in this species.
authors.affiliation: Departmento de Biología Aplicada, Escuela Politécnica Superior, Universidad de Almería, 04120, Almería, Spain.
authors.forename: F J
authors.initials: FJ
authors.lastname: Moyano
authors.forename: M
authors.initials: M
authors.lastname: Díaz
authors.forename: F J
authors.initials: FJ
authors.lastname: Alarcón
authors.forename: M C
authors.initials: MC
authors.lastname: Sarasquete