title: In vitro development of goat-sheep and goat-goat zona-free cloned embryos in different culture media.
abstract: The gradual decline in the genetic diversity of farm animals has threatened their survival and risk of their extinction has increased many fold in the recent past. Endangered species could be rescued using interspecies embryo production. The objective of this study was to investigate the effect of three different culture media on the development of Handmade cloned intraspecies (goat-goat) and interspecies (goat-sheep) embryo reconstructs. Research vitro cleave media (RVCL) yielded higher cleavage and morula-blastocyst development in intraspecies and interspecies nuclear transfer groups compared with G1.G2 and modified synthetic oviductal fluid (mSOFaaci). Cleavage frequency of intraspecies cloned embryos in RVCL, mSOFaaci, and G1.G2 did not differ significantly (87.12%, 82.45%, and 92.52%, respectively). However, the morula/blastocyst frequency in RVCL was greater in mSOFaaci and G1.G2 (51.18% vs. 38.28% vs. 36.50%, respectively). Cleavage and morula/blastocyst frequency in interspecies cloned embryos was greater in RVCL than in mSOFaaci and G1.G2 (76.14% and 42.3% vs. 65.9% and 38.3% vs. 58.56% and 33.1%, respectively). Goat oocytes were parthenogenetically activated and cultured in RVCL, mSOFaaci, and G1.G2 and kept as control. Cleavage and morula/blastocyst frequency in this group was greater in RVCL than in mSOFaaci and G1.G2 (89.66% and 65.26% vs. 85.44% and 48.05% vs. 86.58% and 42.06%, respectively). Conclusively, the results suggest that not only can the interspecies embryos of goat be produced using sheep oocytes as donor cytoplast but also the percentages can be improved by using RVCL media for culturing of the embryos.
authors.affiliation: Centre of Animal Biotechnology, Faculty of Veterinary Science, Sher-e- Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India.
authors.forename: F A
authors.initials: FA
authors.lastname: Khan
authors.affiliation: Centre of Animal Biotechnology, Faculty of Veterinary Science, Sher-e- Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India.
authors.forename: M H
authors.initials: MH
authors.lastname: Bhat
authors.affiliation: Centre of Animal Biotechnology, Faculty of Veterinary Science, Sher-e- Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India.
authors.forename: S H
authors.initials: SH
authors.lastname: Yaqoob
authors.affiliation: Centre of Animal Biotechnology, Faculty of Veterinary Science, Sher-e- Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India.
authors.forename: S M
authors.initials: SM
authors.lastname: Waheed
authors.affiliation: Centre of Animal Biotechnology, Faculty of Veterinary Science, Sher-e- Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India.
authors.forename: N A
authors.initials: NA
authors.lastname: Naykoo
authors.affiliation: Division of Veterinary Surgery and Radiology, Faculty of Veterinary Science, Sher-e- Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India.
authors.forename: H
authors.initials: H
authors.lastname: Athar
authors.affiliation: Mountain Research Centre for Sheep and Goat, Faculty of Veterinary Science, Sher-e- Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India.
authors.forename: H M
authors.initials: HM
authors.lastname: Khan
authors.affiliation: Teaching Veterinary Clinical Services Complex, Faculty of Veterinary Science, Sher-e- Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India.
authors.forename: M R
authors.initials: MR
authors.lastname: Fazili
authors.affiliation: Centre of Animal Biotechnology, Faculty of Veterinary Science, Sher-e- Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India.
authors.forename: N A
authors.initials: NA
authors.lastname: Ganai
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Singla
authors.affiliation: Centre of Animal Biotechnology, Faculty of Veterinary Science, Sher-e- Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India. Electronic address: naipcloningskuastk@gmail.com.
authors.forename: R A
authors.initials: RA
authors.lastname: Shah
mesh_terms: D000818:Animals; D019976:Cloning, Organism / Q000662:veterinary; D003247:Conservation of Natural Resources; D003470:Culture Media; D046149:Embryo Culture Techniques / Q000662:veterinary*; D047108:Embryonic Development; D056727:Endangered Species; D006041:Goats / Q000196:embryology*; D053652:Nuclear Transfer Techniques / Q000662:veterinary; D012756:Sheep / Q000196:embryology*; D015044:Zona Pellucida / Q000502:physiology*
chemical_list: D003470:Culture Media
keywords: Handmade cloning; Interspecies; Intraspecies; Somatic cell nuclear transfer