title: IL-17-producing double-negative T cells are expanded in the peripheral blood, infiltrate the salivary gland and are partially resistant to corticosteroid therapy in patients with Sjögren's syndrome.
abstract: A small CD3+ T-cell population, that lacks both CD4 and CD8 molecules, defined as double negative (DN), is expanded in the peripheral blood of patients with systemic lupus erythematosus, produces IL-17 and accumulates in the kidney during lupus nephritis. Since IL-17 production is enhanced in salivary gland infiltrates of patients with primary Sjögren's syndrome (pSS), we aimed to investigate whether DN T cells may be involved in the pathogenesis of salivary gland damage. Fifteen patients with SS and 15 normal controls (NC) were enrolled. Peripheral blood mononuclear cells were stimulated with anti-CD3 antibody and cultured in presence or absence of dexamethasone (Dex). Phenotypic characterization was performed by flow cytometry in freshly isolated cells and after culture. Minor salivary glands (MSG) from pSS were processed for immunofluorescence staining. Total circulating DN T cells were increased in pSS compared to NC (4.7±0.4% vs 2.6±0.4%). NC and pSS freshly isolated DN T cells produce consistent amounts of IL-17 (67.7±5.6 in NC vs 69.2±3.3 in pSS). Notably, DN T cells were found in the pSS-MSG infiltrate. Dex was able to down-regulate IL-17 in vitro production in NC (29±2.6% vs 15.2±1.9% vs 13±1.6%) and pSS (49±4.8% vs 16±3.8% vs 10.2±0.8%) conventional Th17 cells and in DN T cells of NC (80±2.8% vs 3.8±2.1% vs 4.2±1.8%), but not of pSS (81±1.5% vs 85.4±0.8% vs 86.2±1.7%). DN T cells are expanded in pSS PB, produce IL-17 and infiltrate pSS MSG. In pSS, conventional Th17 cells are inhibited by Dex, but DN T cells appear to be resistant to this effect. Taken together, these data suggest a key role of this T-cell subset in the perpetuation of chronic sialoadenitis and eventually in pSS prognosis.
authors.affiliation: S.S.D. di Reumatologia, Dipartimento di Medicina Clinica e Sperimentale, Università degli Studi di Perugia. gerlir@unipg.it.
authors.forename: A
authors.initials: A
authors.lastname: Alunno
authors.forename: O
authors.initials: O
authors.lastname: Bistoni
authors.forename: E
authors.initials: E
authors.lastname: Bartoloni Bocci
authors.forename: S
authors.initials: S
authors.lastname: Caterbi
authors.forename: B
authors.initials: B
authors.lastname: Bigerna
authors.forename: A
authors.initials: A
authors.lastname: Pucciarini
authors.forename: A
authors.initials: A
authors.lastname: Tabarrini
authors.forename: R
authors.initials: R
authors.lastname: Mannucci
authors.forename: D
authors.initials: D
authors.lastname: Beghelli
authors.forename: B
authors.initials: B
authors.lastname: Falini
authors.forename: R
authors.initials: R
authors.lastname: Gerli
mesh_terms: D017252:CD3 Complex; D016827:CD8 Antigens; D002478:Cells, Cultured; D003907:Dexamethasone / Q000627:therapeutic use*; D004351:Drug Resistance; D005260:Female; D005938:Glucocorticoids / Q000627:therapeutic use*; D006801:Humans; D020381:Interleukin-17 / Q000096:biosynthesis / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012469:Salivary Glands / Q000276:immunology*; D012859:Sjogren's Syndrome / Q000097:blood / Q000188:drug therapy* / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D017252:CD3 Complex; D016827:CD8 Antigens; D005938:Glucocorticoids; D020381:Interleukin-17; D003907:Dexamethasone