title: Anti-tumor activity of a peptide combining patterns of insect alloferons and mammalian immunoglobulins in naïve and tumor antigen vaccinated mice.
abstract: Alloferons are a group of naturally occurring peptides primarily isolated from insects and capable of stimulating mouse and human NK cell cytotoxicity towards cancer cells. In this paper we examined anti-tumor activity of alloferon-1 and its novel structural analog referred to as allostatine. The activity was tested in naïve and preventively tumor antigen vaccinated DBA/2 mice subcutaneously grafted with syngenic P388D1 mouse leukemia cells. In naïve animals allostatine demonstrated tumoristatic activity prevailing over alloferon-1 effect. The preventive vaccination caused only weak tumoristatic effect in 27% of vaccinated animals. The vaccination efficacy was dramatically enhanced by allostatine but not alloferon-1 administration: 65% of allostatine treated animals benefitted from tumoristatic effect and 30% was completely cured so that total number of positive responders grew to 95%. Thus, alloferon-1 and especially allostatine are worthy of further consideration as potential anti-cancer drugs. Allostatine seems to be particularly perspective for adjuvant cancer immunotherapy. Sequence similarity search revealed evolutionary conserved allostatine-like pattern inserted to CDR3 region of human and mouse immunoglobulins. By analogy with allostatine, the pattern may execute some unknown so far function in anti-tumor immune response regulation.
authors.affiliation: Laboratory of Insect Biopharmacology and Immunology, Faculty of Biology and Soil Science, St. Petersburg State University, Oranienbaum str. 2, 198904 St. Petersburg, Russia. Electronic address: sichernysh@yahoo.com.
authors.forename: Sergey
authors.initials: S
authors.lastname: Chernysh
authors.forename: Irina
authors.initials: I
authors.lastname: Kozuharova
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry; D007167:Immunotherapy; D007313:Insecta; D051379:Mice; D008811:Mice, Inbred DBA; D009369:Neoplasms / Q000473:pathology / Q000628:therapy*; D010455:Peptides / Q000737:chemistry / Q000627:therapeutic use*; D016415:Sequence Alignment; D047368:Tumor Burden / Q000187:drug effects
chemical_list: C510535:Alloferon; D000951:Antigens, Neoplasm; D000970:Antineoplastic Agents; D019496:Cancer Vaccines; D007136:Immunoglobulins; D010455:Peptides; C000591159:allostatine
keywords: Alloferons; Allostatine; Cancer immunotherapy; Immunoglobulins; Mouse tumor transplantation model