title: Functional mesenchymal stem cells remain present in bone marrow microenvironment of patients with leukemia post-allogeneic hematopoietic stem cell transplant.
abstract: Mesenchymal stem cells (MSCs) and their progenies are important supporting cells in the bone marrow (BM) microenvironment. However, the function and kinetics of MSCs post-hematopoietic stem cell transplant (HSCT) remain unknown. In the present study, MSCs were cultured from a total of 76 BM samples from 15 patients receiving HSCT. Colony-forming unit fibroblasts in BM before pre-conditioning and 1, 3, 6 and 9 months post-HSCT were cultured and counted to quantify MSCs. Hematopoiesis-supporting activity of MSCs was observed with long-term culture of hematopoietic progenitors. An inhibitory effect of MSCs on in vitro lymphocyte proliferation was also observed. Results showed that post-HSCT MSCs supported in vitro hematopoiesis and inhibited lymphocyte growth. Moreover, the quantity of MSCs was reduced at an early stage and restored to baseline level 9 months post-transplant. The results indicate that functional MSCs remain present in the BM microenvironment, and these findings shed light on the understanding of BM microenvironment reconstitution post-HSCT.
authors.affiliation: Department of Hematology, General Hospital of the Air Force , PLA, Beijing , China.
authors.forename: Li
authors.initials: L
authors.lastname: Ding
authors.forename: Heng
authors.initials: H
authors.lastname: Zhu
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Zhi-Dong
authors.initials: ZD
authors.lastname: Wang
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Zheng
authors.forename: Hong-Min
authors.initials: HM
authors.lastname: Yan
authors.forename: Lei
authors.initials: L
authors.lastname: Dong
authors.forename: Hai-Hong
authors.initials: HH
authors.lastname: Zhang
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Han
authors.forename: Mei
authors.initials: M
authors.lastname: Xue
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Ling
authors.initials: L
authors.lastname: Zhu
authors.forename: Zi-Kuan
authors.initials: ZK
authors.lastname: Guo
authors.forename: Heng-Xiang
authors.initials: HX
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D001853:Bone Marrow / Q000378:metabolism*; D002454:Cell Differentiation; D002648:Child; D002675:Child, Preschool; D003114:Colony-Forming Units Assay; D005260:Female; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D016130:Immunophenotyping; D007938:Leukemia / Q000378:metabolism* / Q000628:therapy; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010641:Phenotype; D055153:Stem Cell Niche; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology; D014019:Tissue Donors; D014184:Transplantation, Homologous; D055815:Young Adult
keywords: Mesenchymal stem cells; allogeneic hematopoietic stem cell transplant; bone marrow microenvironment