title: Preparation of formalin-fixed paraffin-embedded tissue for immunohistochemistry.
abstract: The purpose of this protocol is to take any biopsy or whole organ tissue from animals or human, formalin-fix the specimen to preserve the current state of the tissue, and embed it into a paraffin block and for future immunohistochemistry experiments (If you intend to fix cells, check the alternative protocols: Preparation of Cells for Microscopy using Cytospin, Preparation of Cells for Microscopy using Chamber Slides and Coverslips, or Preparation of Cells for Microscopy using 'Cell Blocks').
authors.affiliation: Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD, USA. Electronic address: kirstieadams26@yahoo.com.
authors.forename: Kirstie
authors.initials: K
authors.lastname: Canene-Adams
mesh_terms: D000818:Animals; D005557:Formaldehyde; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D016612:Paraffin Embedding / Q000295:instrumentation / Q000379:methods*; D016707:Tissue Fixation / Q000295:instrumentation / Q000379:methods*
chemical_list: D005557:Formaldehyde
keywords: Fixation of tissue; Formalin-fixed paraffin-embedded tissue (FFPET); Immunohistochemistry; Tissue process and embedded; Tissue section