title: Cytogenetic analysis of somatic and germinal cells from 38,XX/38,XY phenotypically normal boars.
abstract: Many chromosomal abnormalities have been reported to date in pigs. Most of them have been balanced structural rearrangements, especially reciprocal translocations. A few cases of XY/XX chimerism have also been diagnosed within the national systematic chromosomal control program of young purebred boars carried out in France. Until now, this kind of chromosomal abnormality has been mainly reported in intersex individuals. We investigated 38,XY/38,XX boars presenting apparently normal phenotypes to evaluate the potential effects of this particular chromosomal constitution on their reproductive performance. To do this, we analyzed (1) the chromosomal constitution of cells from different organs in one boar; (2) the aneuploidy rates for chromosomes X, Y, and 13 in sperm nuclei sampled from seven XY/XX boars. 2n = 38,XX cells were identified in different nonhematopoietic tissues including testis (frequency, <8%). Similar aneuploidy rates were observed in the sperm nuclei of XY/XX and normal individuals (controls). Altogether, these results suggest that the presence of XX cells had no or only a very limited effect on the reproduction abilities of the analyzed boars.
authors.affiliation: INRA, UMR 444, Génétique Cellulaire, Toulouse, France; Université de Toulouse, INP, ENVT, UMR 444, Génétique Cellulaire, Toulouse, France.
authors.forename: Harmonie
authors.initials: H
authors.lastname: Barasc
authors.affiliation: GenESI Génétique, Expérimentation et Système Innovants Poitou Charentes, Saint-Pierre-d'Amilly, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Ferchaud
authors.affiliation: INRA, UMR 444, Génétique Cellulaire, Toulouse, France; Université de Toulouse, INP, ENVT, UMR 444, Génétique Cellulaire, Toulouse, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Mary
authors.affiliation: INRA, UMR 444, Génétique Cellulaire, Toulouse, France; Université de Toulouse, INP, ENVT, UMR 444, Génétique Cellulaire, Toulouse, France.
authors.forename: Marie Adélaïde
authors.initials: MA
authors.lastname: Cucchi
authors.affiliation: INRA, UMR 444, Génétique Cellulaire, Toulouse, France; Université de Toulouse, INP, ENVT, UMR 444, Génétique Cellulaire, Toulouse, France.
authors.forename: Amalia Naranjo
authors.initials: AN
authors.lastname: Lucena
authors.affiliation: Université de Toulouse, INP, ENVT, UMS 006, Département des Sciences Biologiques et Fonctionnelles, Laboratoire d'Histopathologie, Toulouse, France.
authors.forename: Isabelle Raymond
authors.initials: IR
authors.lastname: Letron
authors.affiliation: INRA, UMR 444, Génétique Cellulaire, Toulouse, France; Université de Toulouse, INP, ENVT, UMR 444, Génétique Cellulaire, Toulouse, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Calgaro
authors.affiliation: INRA, UMR 444, Génétique Cellulaire, Toulouse, France; Université de Toulouse, INP, ENVT, UMR 444, Génétique Cellulaire, Toulouse, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Bonnet
authors.affiliation: INRA, UMR 444, Génétique Cellulaire, Toulouse, France; Université de Toulouse, INP, ENVT, UMR 444, Génétique Cellulaire, Toulouse, France.
authors.forename: Anne Marie
authors.initials: AM
authors.lastname: Dudez
authors.affiliation: INRA, UMR 444, Génétique Cellulaire, Toulouse, France; Université de Toulouse, INP, ENVT, UMR 444, Génétique Cellulaire, Toulouse, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Yerle
authors.affiliation: INRA, UMR 444, Génétique Cellulaire, Toulouse, France; Université de Toulouse, INP, ENVT, UMR 444, Génétique Cellulaire, Toulouse, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Ducos
authors.affiliation: INRA, UMR 444, Génétique Cellulaire, Toulouse, France; Université de Toulouse, INP, ENVT, UMR 444, Génétique Cellulaire, Toulouse, France. Electronic address: a.pinton@envt.fr.
authors.forename: Alain
authors.initials: A
authors.lastname: Pinton
mesh_terms: D000782:Aneuploidy; D000818:Animals; D046528:Chimerism / Q000662:veterinary*; D017404:In Situ Hybridization, Fluorescence / Q000662:veterinary; D007962:Leukocytes / Q000166:cytology; D008297:Male; D010641:Phenotype; D012098:Reproduction / Q000235:genetics*; D012730:Sex Chromosomes; D019849:Sex Determination Processes; D013094:Spermatozoa; D013552:Swine / Q000235:genetics*; D013553:Swine Diseases / Q000235:genetics*
keywords: Chimerism; Chromosomes; Pig; Sperm