title: Factors regulating tropane-alkaloid production in a transformed root culture of a Datura candida × D. aurea hybrid.
abstract: Using in combination an analysis of (i) the levels of enzyme activities present, (ii) the pool sizes of metabolic intermediates and end products and (iii) the effects of feeding metabolic intermediates, the limitations ℴ flux into tropane alkaloids in a Datura root culture have been examined. This culture, produced by transforming a Datura candida × D. aurea hybrid with Agrobacterium rhizogenes, is found to be highly competent in the biosynthesis of both hyoscyamine and scopolamine as well as a wide range of other hygrine-derived alkaloids. It has been found that, of six enzymes which are involved in this pathway, the two initial activities, ornithine decarboxylase (EC 4.1.1.17) and arginine decarboxylase (EC 4.1.1.19), are present at potentially flux-limiting levels, in contrast to those other enzymes assayed which act further down the pathway. An additional limitation to flux, involving the supply of activated acids for condensation with tropine to form the identified tropoyl and tigloyl derivatives, is also indicated from the observed effect of feeding free acids. The relative contribution to flux limitation caused by these two interacting phenomena is inferred from an analysis of the changing relative levels of metabolic intermediates and end products as cultures mature.
authors.affiliation: Plant Biotechnology Group, Department of Genetics and Microbiology, AFRC Institute of Food Research, Colney Lane, NR4 7UA, Norwich, UK.
authors.forename: R J
authors.initials: RJ
authors.lastname: Robins
authors.forename: A J
authors.initials: AJ
authors.lastname: Parr
authors.forename: J
authors.initials: J
authors.lastname: Payne
authors.forename: N J
authors.initials: NJ
authors.lastname: Walton
authors.forename: M J
authors.initials: MJ
authors.lastname: Rhodes