title: Collision induced decomposition of peptides. Choice of collision parameters.
abstract: Collision-induced dissociation product ion spectra of a series of doubly charged tryptic peptide ions produced by electrospray ionization were obtained by triple-quadrupole tandem mass spectrometry. The sequence information content of the product ion spectra was explored as a function of collision energy and collision-cell gas pressure for parent ions with molecular masses ranging from 300 to 2000 u. The energy range (at a given pressure) in which the degree of fragmentation is acceptable was found to be narrow for parent ions of a given mass, and the optimal collision energy was observed to exhibit a strong linear correlation with parent ion mass. This observed correlation opens the way for on-line software-controled selection of optimal mass spectrometric conditions in the enzymatic digestion-liquid chromatography-tandem mass spectrometric strategy of amino acid sequencing of proteins.
authors.affiliation: Mass Spectrometry Laboratory, The Rockefeller University, 1230 York Avenue, New York, New York, USA.
authors.forename: I
authors.initials: I
authors.lastname: Haller
authors.forename: U A
authors.initials: UA
authors.lastname: Mirza
authors.forename: B T
authors.initials: BT
authors.lastname: Chait