title: Investigating the reactivities of a polyketide synthase module through fluorescent click chemistry.
abstract: A method for monitoring in vitro polyketide synthesis has been developed whereby nonchromophoric polyketide products are made brightly fluorescent in a simple, rapid, inexpensive, and bioorthogonal manner through CuAAC with a sulforhodamine B azide derivative.
authors.affiliation: Department of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station A5300, Austin, TX, USA. adriankc@utexas.edu.
authors.forename: Amanda Jane
authors.initials: AJ
authors.lastname: Hughes
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Tibby
authors.forename: Drew T
authors.initials: DT
authors.lastname: Wagner
authors.forename: Johnathan N
authors.initials: JN
authors.lastname: Brantley
authors.forename: Adrian T
authors.initials: AT
authors.lastname: Keatinge-Clay
mesh_terms: D001386:Azides / Q000737:chemistry*; D002384:Catalysis; D057930:Click Chemistry; D003300:Copper / Q000737:chemistry; D005456:Fluorescent Dyes; D048630:Polyketide Synthases / Q000378:metabolism*; D061065:Polyketides / Q000378:metabolism*; D012235:Rhodamines
chemical_list: D001386:Azides; D005456:Fluorescent Dyes; D061065:Polyketides; D012235:Rhodamines; C022027:lissamine rhodamine B; D003300:Copper; D048630:Polyketide Synthases