title: Real-time in vivo imaging reveals the ability of monocytes to clear vascular amyloid beta.
abstract: Alzheimer's disease (AD) is characterized by the accumulation of amyloid beta (Aβ) that is assumed to result from impaired elimination of this neurotoxic peptide. Most patients with AD also exhibit cerebral amyloid angiopathy, which consists of Aβ deposition within the cerebral vasculature. The contribution of monocytes in AD has so far been limited to macrophage precursors. In this study, we aimed to investigate whether circulating monocytes could play a role in the elimination of Aβ. With live intravital two-photon microscopy, we demonstrate that patrolling monocytes are attracted to and crawl onto the luminal walls of Aβ-positive veins, but not on Aβ-positive arteries or Aβ-free blood vessels. Additionally, we report the presence of crawling monocytes carrying Aβ in veins and their ability to circulate back into the bloodstream. Selective removal of Ly6C(lo) monocytes in APP/PS1 mice induced a significant increase of Aβ load in the cortex and hippocampus. These data uncover the ability of Ly6C(lo) monocytes to naturally target and eliminate Aβ within the lumen of veins and constitute a potential therapeutic target in AD.
authors.affiliation: Neuroscience Laboratory, CHU de Québec Research Center, Department of Molecular Medicine, Laval University, 2705 Laurier Boulevard, Québec G1V 4G2, Canada.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Michaud
authors.forename: Marc-André
authors.initials: MA
authors.lastname: Bellavance
authors.forename: Paul
authors.initials: P
authors.lastname: Préfontaine
authors.forename: Serge
authors.initials: S
authors.lastname: Rivest
mesh_terms: D000544:Alzheimer Disease / Q000097:blood / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000097:blood*; D000818:Animals; D001808:Blood Vessels / Q000378:metabolism / Q000473:pathology; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods
chemical_list: D016229:Amyloid beta-Peptides