title: Simple segmental hair analysis for α-pyrrolidinophenone-type designer drugs by MonoSpin extraction for evaluation of abuse history.
abstract: For detection of a history of drug abused, we developed a simple method for extracting pyrrolidinophenone-type designer drugs in human hair by using a MonoSpin(®) C18 column. Target drugs were extracted from a single alkaline-digested hair segment (length, 10mm; weight, ca 0.1mg). The analytes extracted were then analyzed by high-performance liquid chromatography-mass spectrometry without evaporation of the eluent after MonoSpin extraction. Linearity from 0.5 to 500ng/mg was observed for all the tested drugs using an internal standard method (correlation coefficients >0.998) and the limit of detection was 0.2ng/mg. The recoveries were between 0.7 and 11.1%. The coefficients for intraday and interday variations at 4, 40, 200, and 400ng/mg in hair were between 0.7 and 11.1%. This method was successfully applied to the identification of these designer drugs in segmented human hair from drug abusers and indicated their history of drug abuse. The results were consistent with the patients' statements, indicating that this rapid method can be used to detect a history of drug abuse.
authors.affiliation: Department of Forensic Medicine, Institute of Biomedical & Health Sciences, Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8553, Japan. Electronic address: namera@hiroshima-u.ac.jp.
authors.forename: Akira
authors.initials: A
authors.lastname: Namera
authors.forename: Kyohei
authors.initials: K
authors.lastname: Konuma
authors.forename: Takeshi
authors.initials: T
authors.lastname: Saito
authors.forename: Shigenori
authors.initials: S
authors.lastname: Ota
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Oikawa
authors.forename: Shota
authors.initials: S
authors.lastname: Miyazaki
authors.forename: Shumari
authors.initials: S
authors.lastname: Urabe
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Shiraishi
authors.forename: Masataka
authors.initials: M
authors.lastname: Nagao
mesh_terms: D052117:Benzodioxoles / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D015198:Designer Drugs / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D006197:Hair / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D013058:Mass Spectrometry; D011759:Pyrrolidines / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D015813:Substance Abuse Detection / Q000379:methods*; D000094982:Synthetic Cathinone
chemical_list: D052117:Benzodioxoles; D015198:Designer Drugs; D011759:Pyrrolidines; C542924:alpha-pyrrolidinovalerophenone; D000094982:Synthetic Cathinone
keywords: Designer drug; Hair analysis; MonoSpin; Pyrrolidinophenone