title: High fat diet induced insulin resistance and elevated retinol binding protein 4 in female rats; treatment and protection with Berberis vulgaris extract and vitamin A.
abstract: This research was conducted to investigate two main aims; the first aim was to find if there is a relationship between insulin resistance (IR) and retinol binding protein 4 (RBP4). The second aim was to use berberis vulgaris extract and vitamin A as protective and/or curative agents against insulin resistance. IR was developed by feeding the female rats a high fat diet (HFD) for six weeks then treating or protecting them with b. vulgaris extract (0.2 g/Kg body weight) or vitamin A (12.8μg/Kg/day) for two weeks. HFD intake elevated insulin level and RBP4 expression that associated with hyperglycemia and hyperlipidemia. Co-administration of vitamin A and B. vulgaris extracts reduced blood glucose level, insulin, body weight and RBP4 expression before, during and after HFD. Furthermore, vitamin A reduced the blood glucose, triglycerides (TG) and cholesterol levels. IR syndrome associated with the RBP 4 alteration that gives high indication about the role of RBP4 expression in the IR progression and development. Furthermore, the treatment with vitamin A and/or b. vulgaris alleviated the IR syndrome through the action on RBP4 and Insulin secretion. On the other hand, vitamin A must be avoided for the predisposed IR and prediabetic patients.
authors.affiliation: Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
authors.forename: Mohamed Mohammed
authors.initials: MM
authors.lastname: El-Sayed
authors.forename: Doaa Ahmad
authors.initials: DA
authors.lastname: Ghareeb
authors.forename: Heba Allah
authors.initials: HA
authors.lastname: Talat
authors.forename: Eman Mohammed
authors.initials: EM
authors.lastname: Sarhan
mesh_terms: D000821:Animal Feed; D000818:Animals; D028142:Berberis; D059305:Diet, High-Fat; D005260:Female; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008055:Lipids / Q000097:blood; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D054839:Retinol-Binding Proteins, Plasma / Q000502:physiology*; D014801:Vitamin A / Q000627:therapeutic use*
chemical_list: D007328:Insulin; D008055:Lipids; D010936:Plant Extracts; C532035:Rbp4 protein, rat; D054839:Retinol-Binding Proteins, Plasma; D014801:Vitamin A