title: Sonic hedgehog pathway dysregulation in skin basal-cell carcinoma of a Polish population.
abstract: Sonic hedgehog (Shh) pathway impairment plays a key role in the pathogenesis of basal-cell carcinomas (BCC), the most frequent skin tumor among Caucasians. Shh, Smo, and Gli2 family proteins are necessary for adequate and controlled cell proliferation. The aim of this study was to evaluate Shh, Smo, and Smo expression in BCC skin biopsies taken from sun-exposed areas. 41 BCC skin biopsies and 22 healthy skin specimens (the control group) taken from the same areas served as material for the study. All specimens were immunohistochemically stained with monoclonal antibodies directed against the chosen proteins. Shh and Smo expression (cytoplasmic pattern) were recorded semiquantitatively using a four-grade score (0-3). Gli2 expression (nuclear pattern) was determined using an image analysis system (semiautomatic function). The immunoexpression of the Shh and Smo proteins significantly increased in the BCC group, as compared with the normal controls (for Shh, the mean intensity was 1.67 in BCC vs. 1.17 in the control group, p < 0.001; for Smo, the mean intensity was 1.46 in BCC vs. 0.99 in the control group, p < 0.001). The staining for Gli2 in the BCC group was completely negative, but indicated the presence of Gli2 in the control patients (1.15 Gli2+ cells/100 cells). Sonic hedgehog pathway dysregulation may play an important role in skin cancerogenesis leading to BCC development.
authors.affiliation: Department of Dermatology Medical University of Lodz. lesiak_ola@interia.pl.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Lesiak
authors.forename: Dorota
authors.initials: D
authors.lastname: Sobolewska-Sztychny
authors.forename: Marian
authors.initials: M
authors.lastname: Danilewicz
authors.forename: Michal
authors.initials: M
authors.lastname: Rogowski-Tylman
authors.forename: Anna
authors.initials: A
authors.lastname: Sysa-Jedrzejowska
authors.forename: Michal
authors.initials: M
authors.lastname: Sobjanek
authors.forename: Irmina
authors.initials: I
authors.lastname: Olejniczak-Staruch
authors.forename: Joanna
authors.initials: J
authors.lastname: Narbutt
mesh_terms: D002280:Basal Cell Carcinoma / Q000175:diagnosis / Q000209:etiology / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D053823:Hedgehog Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011044:Poland; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D012878:Skin Neoplasms / Q000175:diagnosis / Q000209:etiology / Q000378:metabolism*; D000072086:Smoothened Receptor; D013472:Sunlight / Q000009:adverse effects; D000075746:Zinc Finger Protein Gli2
chemical_list: C082593:GLI2 protein, human; D053823:Hedgehog Proteins; D051741:Kruppel-Like Transcription Factors; D009687:Nuclear Proteins; D043562:Receptors, G-Protein-Coupled; C509239:SHH protein, human; C491023:SMO protein, human; D000072086:Smoothened Receptor; D000075746:Zinc Finger Protein Gli2