title: Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics.
abstract: Here, we present a platform to detect cytokine (IL-2, IFN-γ, TNF-α) secretion of single, activated T-cells in droplets over time. We use a novel droplet-based microfluidic approach to encapsulate cells in monodisperse agarose droplets together with functionalized cytokine-capture beads for subsequent binding and detection of secreted cytokines from single cells. This method allows high-throughput detection of cellular heterogeneity and maps subsets within cell populations with specific functions.
authors.affiliation: Institute for Molecules and Materials, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands. w.huck@science.ru.nl.
authors.forename: Venkatachalam
authors.initials: V
authors.lastname: Chokkalingam
authors.forename: Jurjen
authors.initials: J
authors.lastname: Tel
authors.forename: Florian
authors.initials: F
authors.lastname: Wimmers
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Sergey
authors.initials: S
authors.lastname: Semenov
authors.forename: Julian
authors.initials: J
authors.lastname: Thiele
authors.forename: Carl G
authors.initials: CG
authors.lastname: Figdor
authors.forename: Wilhelm T S
authors.initials: WT
authors.lastname: Huck
mesh_terms: D002214:Capsules; D002470:Cell Survival; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism*; D013997:Time Factors
chemical_list: D002214:Capsules; D016207:Cytokines