title: Redundancy modulation of nuclear DNA sequences in Dasypyrum villosum.
abstract: In order to assess fluid domains in the genome of Dasypyrum villosum, Feulgen/DNA cytophotometric determinations and molecular and cytological DNA-DNA hybridization experiments were carried out in resting embryos and developing seedlings from yellow and brown caryopses belonging to different populations. The cytophotometric data showed that the basic amount of nuclear DNA is, on average, 12% higher in 2-day-old seedlings from yellow caryopses as compared to those from brown caryopses. It increases in each individual during seed germination, to a higher extent in seedlings from yellow caryopses than in those from brown caryopses. DNA content also differs up to 13% between plants within a caryopsis-colour group and up to 40% between populations. Dot-blot hybridization of a 396-bp D. villosum-specific DNA repeat to genomic DNA extracted from embryos in dry seeds, or from seedlings belonging to single progenies of plants from different populations, confirmed the cytophotometric results. The redundancy in the genome of sequences hybridizing to the 396-bp element differs significantly both between populations and between plant progenies within a population. During seed germination these sequences are the more amplified the less they are redundant in the genome of resting embryos, and amplification occurs to a significantly-greater extent in seedlings from yellow caryopses than in those from brown caryopses. (3)H-labelled 396-bp sequences hybridize at or near the telomeres of most chromsome pairs though only to the shorter of the two subtelocentric pairs. The hybridization level is higher in seedlings from yellow caryopses that in those from brown caryopses, and a linear correlation exists between the number of silver grains counted over the labelled regions of each chromosome pair in the two groups of seedlings. Possible control mechanisms of the observed changes in the nuclear genome, and the role of these changes in developmental pregulation and environmental adaptation, are discussed.
authors.affiliation: Dipartimento di Scienze Botaniche della Università, Via L Ghini 5, I-56124, Pisa, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Frediani
authors.forename: N
authors.initials: N
authors.lastname: Colonna
authors.forename: R
authors.initials: R
authors.lastname: Cremonini
authors.forename: C
authors.initials: C
authors.lastname: De Pace
authors.forename: V
authors.initials: V
authors.lastname: Delre
authors.forename: R
authors.initials: R
authors.lastname: Caccia
authors.forename: P G
authors.initials: PG
authors.lastname: Cionini