title: Chemical constituents from Aphanamixis grandifolia.
abstract: Four new terpenoids, nemoralisins D-G (1-4), were isolated from the leaves and stems of Aphanamixis grandifolia, along with two known diterpenoids, nemoralisin C and nemoralisin. Among them, compound 1 is the first example of norsesquiterpenoid with δ-lactone moiety, and nemoralisins E-G (2-4), are a class of acyclic diterpenoids, which are structurally related nemoralisin C and nemoralisin. These structures were established on the basis of spectroscopic methods and the absolute configuration of 1 was determined by comparison of quantum chemical TDDFT calculated and experimental ECD spectra. Nemoralisins D-G (1-4) were tested for their cytotoxicities on HL-60, SMMC-7721, A-549, MCF-7, and SW480 human tumor cell lines (IC50>40 μM), as well as the antimicrobial activities on Staphylococcus aureus, Pseudomonas aeruginosa, MRSA92(#) and MRSA98(#) (MIC>50 μg/mL).
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201 Yunnan, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201 Yunnan, PR China.
authors.forename: Hong-Ping
authors.initials: HP
authors.lastname: He
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201 Yunnan, PR China.
authors.forename: Ying-Tong
authors.initials: YT
authors.lastname: Di
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201 Yunnan, PR China.
authors.forename: Shun-Lin
authors.initials: SL
authors.lastname: Li
authors.affiliation: Research Center of Natural Medicine, Clinical School of Kunming General Hospital of Chengdu Military Command, Kunming 650032, PR China.
authors.forename: Guo-Ying
authors.initials: GY
authors.lastname: Zuo
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201 Yunnan, PR China. Electronic address: zhangyu@mail.kib.ac.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201 Yunnan, PR China. Electronic address: haoxj@mail.kib.ac.cn.
authors.forename: Xiao-Jiang
authors.initials: XJ
authors.lastname: Hao
mesh_terms: D001419:Bacteria / Q000187:drug effects; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D061986:MCF-7 Cells; D029582:Meliaceae / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D004224:Diterpenes; D010936:Plant Extracts
keywords: Aphanamixis grandifolia; Diterpenoid; Nemoralisins D–G; TDDFT