title: Purification and antioxidant properties of octapeptide from salmon byproduct protein hydrolysate by gastrointestinal digestion.
abstract: Pectoral fin protein from salmon processing byproduct was hydrolyzed using Alcalase, Flavourzyme, Neutrase, pepsin, Protamex, and trypsin, and the peptic hydrolysate showed the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Antioxidant peptide was purified using consecutive chromatography. The purified antioxidant peptide was identified to be Phe-Leu-Asn-Glu-Phe-Leu-His-Val with molecular weight of 1018.48 Da by time of flight-mass spectrometry/mass spectrometry (TOF-MS) analysis. The IC50 values against DPPH and 2,2-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) cation radical scavenging activity were 486 and 152 μM, respectively, and the octapeptide showed strong ferric reducing power. In addition, the octapeptide showed significant (p<0.05) protection ability against hydroxyl radical-induced DNA damage and hydrogen peroxide-induced hepatic damage in Chang liver cells. Taken together, the pectoral fin protein hydrolysate and/or its active peptides may be useful ingredients in functional food.
authors.affiliation: Division of Food and Nutrition, Chonnam National University, Gwangju 550-757, Republic of Korea.
authors.forename: Chang-Bum
authors.initials: CB
authors.lastname: Ahn
authors.forename: Jeong-Gyun
authors.initials: JG
authors.lastname: Kim
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Je
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D004063:Digestion; D005396:Fish Products / Q000032:analysis*; D029941:Fish Proteins / Q000737:chemistry*; D006801:Humans; D007220:Industrial Waste / Q000032:analysis*; D018384:Oxidative Stress; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D011492:Protein Hydrolysates / Q000737:chemistry; D012474:Salmon
chemical_list: D000975:Antioxidants; D029941:Fish Proteins; D007220:Industrial Waste; D010455:Peptides; D011492:Protein Hydrolysates
keywords: Antioxidant activity; Enzymatic hydrolysis; Peptide; Protein hydrolysate