title: IgE by itself affects mature rat mast cell preformed and de novo-synthesized mediator release and amplifies mast cell migratory response.
abstract: BACKGROUND
  Immunoglobulin E (IgE) binds to high affinity receptor FcεRI numerously expressed on mast cells. Recent findings have revealed that IgE by itself may regulate various aspects of mast cell biology, however, detailed data is still limited.
  
  
  RESULTS
  Here, we have examined the influence of IgE alone, used at different concentrations, on mast cell activity and releasability. For the study we have employed in vivo differentiated mature tissue mast cells isolated from rat peritoneal cavity. Mast cells were exposed to IgE alone and then the release of preformed and de novo-synthesized mediators, surface FcεRI expression and mast cell migratory response were assessed. IgE by itself was found to up-regulate FcεRI expression and activate mast cells to degranulation, as well as de novo synthesis and release of cysteinyl leukotrienes and TNF. We have provided evidence that IgE alone also amplified spontaneous and CCL5- or TNF-induced migration of mast cells. Importantly, IgE was effective only at concentrations ≥ 3 µg/mL. A molecular basis investigation using an array of specific inhibitors showed that Src kinases, PLC/PLA2, MAP kinases (ERK and p38) and PI3K were entirely or partially involved in IgE-induced mast cell response. Furthermore, IgE alone stimulated the phosphorylation of MAP kinases and PI3K in rat mast cells.
  
  
  CONCLUSIONS
  Our results clearly demonstrated that IgE by itself, at higher concentrations, influences mast cell activity and releasability. As there are different conditions when the IgE level is raised it might be supposed that in vivo IgE is one of the important factors modulating mast cell biology within tissues.
authors.affiliation: Department of Experimental Immunology, Medical University of Łódź, Łódź, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Słodka
authors.forename: Magdalena
authors.initials: M
authors.lastname: Wiktorska
authors.forename: Ewa
authors.initials: E
authors.lastname: Brzezińska-Błaszczyk
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002633:Chemotaxis / Q000187:drug effects; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D007073:Immunoglobulin E / Q000494:pharmacology*; D020935:MAP Kinase Signaling System; D008407:Mast Cells / Q000187:drug effects*; D017208:Rats, Wistar; D017455:Receptors, IgE / Q000378:metabolism
chemical_list: C553474:FCER1A protein, rat; D017455:Receptors, IgE; D007073:Immunoglobulin E