title: An interchangeable system of hairy root and cell suspension cultures ofCatharanthus roseus for indole alkaloid production.
abstract: Hairy roots ofCatharanthus roseus obtained by co-cultivation of hypocotyl segments withAgrobacterium rhizogenes, and cultured in SH (Schenk and Hildebrandt) basal medium, formed two types of calli when subcultured in SH medium with 1 mg/1 α-naphthaleneacetic acid and 0.1 mg/l kinetin. One of them, a compact callus, when re-subcultured in SH basal medium gave rise to hairy roots again. A rhizogenic cell suspension culture was established from this type of callus. When cultured in SH medium with growth regulators, the rhizogenic callus produced catharanthine at a level of 41% of the level in the initial hairy roots. Upon transfer to SH basal medium, regenerated hairy roots produced this alkaloid at the original level of 1.5 mg/g dry wt. Using this cell/hairy root interchange system a new management system for hairy root culture in bioreactors has been devised and examined involving production of biomass in the form of a cell suspension in medium supplemented with growth regulators, and catharanthine production by hairy roots regenerated from these cells in medium without growth regulators.
authors.affiliation: Bioresources Research Group, Korea Research Institute of Bioscience and Biotechnology, Korea Institute of Science and Technology, P.O. Box 115, 305-600, Yusong, Taejon, Korea.
authors.forename: K H
authors.initials: KH
authors.lastname: Jung
authors.forename: S S
authors.initials: SS
authors.lastname: Kwak
authors.forename: C Y
authors.initials: CY
authors.lastname: Choi
authors.forename: J R
authors.initials: JR
authors.lastname: Liu