title: A novel microfluidic chip for assessing dynamic adhesion behavior of cell-targeting microbubbles.
abstract: The primary aim of this study was to develop a microfluidic chip to study the dynamic adhesion behavior of cell-targeted microbubbles. The microfluidic device is composed of polydimethylsiloxane and is fabricated using the soft lithography technique. Each chamber of the microfluidic chip comprises eight U-shaped microsieves, by which various flow velocity distributions are generated. LyP-1-conjugated microbubbles were prepared by coating the surface of the phospholipid shell of microbubbles with LyP-1 peptides via biotin-avidin linkage. Under static conditions, the resulting targeted microbubbles are able to bind onto the surface of cells on incubation with breast cancer cells. Under dynamic fluid conditions, the cell targeting efficiency of the microbubbles was assessed at various flow velocity distributions in a chamber. Accumulation of targeted microbubbles was strongly influenced by flow velocity. Better retention of targeted microbubbles on cell surfaces was achieved at low mean flow velocities (<0.03 cm/s), in agreement with our computer simulation results. In conclusion, our results indicate that the microfluidic system is a useful platform for studying the microbubble-cell adhesive interaction.
authors.affiliation: Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yan
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Jiang
authors.forename: Qiaofeng
authors.initials: Q
authors.lastname: Jin
authors.forename: Zidong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Robin
authors.initials: R
authors.lastname: Shandas
authors.forename: Junru
authors.initials: J
authors.lastname: Wu
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Hairong
authors.initials: H
authors.lastname: Zheng
mesh_terms: D000268:Adhesiveness; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry*; D002469:Cell Separation / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation*; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D045423:Microbubbles; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*
chemical_list: D008055:Lipids
keywords: Dynamic adhesion; LyP-1 peptides; Microfluidic chip; Targeted microbubbles