title: Green fluorescent protein: an in vivo reporter of plant gene expression.
abstract: Protoplasts were isolated from H89, an embryogenic sweet orange (Citrus sinensis (L.) Osbeck cv. Hamlin) suspension culture, and electroporated with p35S-GFP, a plasmid carrying the gene for the green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea victoria. p35S-GFP was constructed by replacing the GUS coding sequence of pBI221 with a functional GFP gene, thereby placing the GFP gene under the control of the CaMV 35S promoter. Protoplasts were viewed by incident-light fluorescence microscopy twentyfour h after electroporation. 20-60% of the protoplasts emitted an intense green light when illuminated with blue (450-490 nm) light.
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Horticultural Research Laboratory, 2120 Camden Road, 32803, Orlando, FL, USA.
authors.forename: R P
authors.initials: RP
authors.lastname: Niedz
authors.forename: M R
authors.initials: MR
authors.lastname: Sussman
authors.forename: J S
authors.initials: JS
authors.lastname: Satterlee