title: Maternal plasma miRNAs expression in preeclamptic pregnancies.
abstract: OBJECTIVE
  Preeclampsia (PE) is a pregnancy-specific syndrome and one of the leading causes of maternal and fetal morbidity and mortality. The pathophysiological mechanisms of PE remain poorly known. Recently, circulating miRNAs are considered as potential useful noninvasive biomarkers. The aim of this study was to identify differentially expressed plasma miRNAs in preeclamptic pregnancies compared with normal pregnancies.
  
  
  METHODS
  Maternal plasma miRNA expression profiles were detected by SOLiD sequencing. Differential expressions between mPE/sPE and control group were found. Next, four differentially expressed plasma miRNAs were chosen to validate their expression in other large scale samples by real-time PCR.
  
  
  RESULTS
  In terms of sequencing results, we identified that 51 miRNAs were differentially expressed. Four differentially expressed plasma miRNAs (miR-141, miR-144, miR-221, and miR-29a) were selected to validate the sequencing results. RT-PCR data confirmed the reliability of sequencing results. The further statistical analysis showed that maternal plasma miR-141 and miR-29a are significantly overexpressed in mPE (P < 0.05). Maternal plasma miR-144 is significantly underexpressed in mPE and sPE (P < 0.05).
  
  
  CONCLUSIONS
  Results showed that there were differentially expressed maternal plasma miRNAs in patients with preeclampsia. These plasma miRNAs might be used as notable biomarkers for diagnosis of preeclampsia.
authors.affiliation: Department of Obstetrics and Gynecology, Zhongda Hospital, Southeast University, Dingjiaqiao 87, Nanjing 210009, China ; State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Sipailou 2, Nanjing 210096, China.
authors.forename: Hailing
authors.initials: H
authors.lastname: Li
authors.forename: Qinyu
authors.initials: Q
authors.lastname: Ge
authors.forename: Li
authors.initials: L
authors.lastname: Guo
authors.forename: Zuhong
authors.initials: Z
authors.lastname: Lu
mesh_terms: D000328:Adult; D001483:Base Sequence; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D015536:Down-Regulation; D005260:Female; D005819:Genetic Markers; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics*; D008969:Molecular Sequence Data; D011225:Pre-Eclampsia / Q000097:blood* / Q000235:genetics*; D011247:Pregnancy; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D015415:Biomarkers; D005819:Genetic Markers; D035683:MicroRNAs; C533895:MIRN141 microRNA, human; C554293:MIRN144 microRNA, human; C525709:MIR221, human; C532045:MIRN29a microRNA, human