title: Concord grape pomace polyphenols complexed to soy protein isolate are stable and hypoglycemic in diabetic mice.
abstract: Polyphenols extracted from Concord grape pomace were stabilized by complexation to soy protein isolate (SPI) to produce grape polyphenol-SPI complex (GP-SPI) containing 5% or 10% grape polyphenols. LC-MS and MALDI-TOF analysis showed that a broad range of phytochemicals were present in the grape pomace extract. Anthocyanins and total polyphenols in the GP-SPI complex were stable after a 16-week incubation at 37 °C but were reduced by up 60% in dried grape pomace extract. Compared to vehicle (236 ± 34 mg/dL), a single dose of 300 mg/kg GP-SPI (184 ± 32 mg/dL) or 500 mg/kg GP-SPI (177 ± 28 mg/dL) having 5% grape polyphenols significantly lowered blood glucose in obese and hyperglycemic C57BL/6 mice 6 h after administration. GP-SPI allows the capture of grape pomace polyphenols in a protein-rich food matrix and may be useful as a functional food ingredient for the management of blood glucose levels.
authors.affiliation: School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey , Foran Hall, 59 Dudley Road, New Brunswick, New Jersey 08901, United States.
authors.forename: Diana E
authors.initials: DE
authors.lastname: Roopchand
authors.forename: Peter
authors.initials: P
authors.lastname: Kuhn
authors.forename: Christian G
authors.initials: CG
authors.lastname: Krueger
authors.forename: Kristin
authors.initials: K
authors.lastname: Moskal
authors.forename: Mary Ann
authors.initials: MA
authors.lastname: Lila
authors.forename: Ilya
authors.initials: I
authors.lastname: Raskin
mesh_terms: D000818:Animals; D001786:Blood Glucose; D005591:Chemical Fractionation / Q000379:methods; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D004355:Drug Stability; D006943:Hyperglycemia / Q000188:drug therapy / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D059808:Polyphenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D044945:Proanthocyanidins / Q000032:analysis; D030262:Soybean Proteins / Q000737:chemistry / Q000494:pharmacology*; D027843:Vitis / Q000737:chemistry*
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D059808:Polyphenols; D044945:Proanthocyanidins; D030262:Soybean Proteins