title: Somatic embryogenesis and plant development from immature zygotic embryos of seedless grapes (Vitis vinifera L.).
abstract: Somatic embryo formation occurred from immature zygotic embryos within ovules of stenospermocarpic seedless grapes (Vitis vinifera L.), when cultured for two months on liquid Emershad/Ramming medium. Somatic embryos continued to proliferate after excision and transfer to Emershad/Ramming medium supplemented with 1 μM benzylaminopurine and 0.65% TC agar. Plant development from somatic embryos was influenced by genotype, medium, phase (liquid, agar), stage (torpedo, mature) and their interactions. Optimal plant development occurred on Woody Plant Medium supplemented with 1.5% sucrose + 1 μM benzylaminopurine + 0.3% activated charcoal and 0.65% TC agar.
authors.affiliation: USDA, ARS, HCRL, 2021 South Peach Avenue, 93727, Fresno, CA, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Emershad
authors.forename: D W
authors.initials: DW
authors.lastname: Ramming