title: Microparticles of Aloe vera/vitamin E/chitosan: microscopic, a nuclear imaging and an in vivo test analysis for burn treatment.
abstract: The use of drug-loaded nanoparticles and microparticles has been increasing, especially for cosmetic and drug delivery purposes. In this work, a new microparticle formulation was developed for use in the healing process of skin burns in a composition of Aloe vera/vitamin E/chitosan. In order to observe the morphological properties, Raman and atomic force microscopy evaluation were performed. The biodistribution studies were analyzed by using a nuclear methodology, labeling the microparticles with Technetium-99m and in vivo test was procedure to analyzed the cicatrization process. The results of AFM analysis show the formation and the adherence property of the microparticles. Raman analyses show the distribution of each component in the microparticle. The nuclear method used shows that the biodistribution of the microparticles remained in the skin. The in vivo cicatrization test showed that the poloxamer gel containing the microparticles make a better cicatrization in relation to the other formulations tested.
authors.affiliation: Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: Gabriela Garrastazu
authors.initials: GG
authors.lastname: Pereira
authors.affiliation: The Laboratory of Nanoradiopharmaceuticals, Instituto de Energia Nuclear, Rio de Janeiro, Brazil.
authors.forename: Ralph
authors.initials: R
authors.lastname: Santos-Oliveira
authors.affiliation: The Laboratory of Nanoradiopharmaceuticals, Instituto de Energia Nuclear, Rio de Janeiro, Brazil.
authors.forename: Martha S
authors.initials: MS
authors.lastname: Albernaz
authors.affiliation: Universidade Federal do Rio de Janeiro, Brazil.
authors.forename: Daniel
authors.initials: D
authors.lastname: Canema
authors.affiliation: Universidade Federal do Rio de Janeiro, Brazil.
authors.forename: Gilberto
authors.initials: G
authors.lastname: Weismüller
authors.affiliation: Universidade Federal do Ceará, Brazil.
authors.forename: Eduardo Bede
authors.initials: EB
authors.lastname: Barros
authors.affiliation: Universidade Federal do Ceará, Brazil.
authors.forename: Luciana
authors.initials: L
authors.lastname: Magalhães
authors.affiliation: Universidade Federal de Pernambuco, Recife, Brazil.
authors.forename: Maria Helena Madruga
authors.initials: MH
authors.lastname: Lima-Ribeiro
authors.affiliation: Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: Adriana Raffin
authors.initials: AR
authors.lastname: Pohlmann
authors.affiliation: Universidade Federal do Rio Grande do Sul, Brazil. Electronic address: silvia.guterres@ufrgs.br.
authors.forename: Silvia S
authors.initials: SS
authors.lastname: Guterres
mesh_terms: D000504:Aloe / Q000737:chemistry*; D000818:Animals; D002056:Burns / Q000188:drug therapy*; D002626:Chemistry, Pharmaceutical / Q000379:methods; D048271:Chitosan / Q000008:administration & dosage / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods; D005260:Female; D005782:Gels / Q000008:administration & dosage / Q000737:chemistry; D008297:Male; D051379:Mice; D008863:Microspheres; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D010316:Particle Size; D012867:Skin / Q000378:metabolism; D014018:Tissue Distribution; D014810:Vitamin E / Q000008:administration & dosage* / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D005782:Gels; D014810:Vitamin E; D048271:Chitosan
keywords: Atomic force microscopy; Burn; In vivo cicatrization; Skin; Technetium-99m