title: Genetics of gliadins coded by the group 1 chromosomes in the high-quality bread wheat cultivar Neepawa.
abstract: The inheritance and biochemical properties of gliadins controlled by the group 1 chromosomes of the high-quality bread wheat cultivar Neepawa were studied in the progeny of the cross Neepawa x Costantino by six different electrophoretic procedures. Chromosome 1B of Neepawa contains two gliadin loci, one (Gli-B1) coding for at least six ω- or γ-gliadins, the other (Gli-B3) controlling the synthesis of gliadin N6 only. The map distance between these loci was calculated as 22.1 cM. Amongst the chromosome 1A gliadins, three proteins are encoded at the Gli-A1 locus whereas polypeptides N14-N15-N16 are controlled by a remote locus which recombines with Gli-A1. Six other gliadins are controlled by a gene cluster at Gli-D1 on chromosome 1D. Canadian wheat cultivars sharing the Gli-B1 allele of Neepawa were found to differ in the presence or absence of gliadin N6. The electrophoretic mobilities of proteins N6 and N14-N15-N16 were unaffected by the addition of a reducing agent during two-dimensional sodium dodecyl sulphate polyacrylamid-gel electrophoresis, suggesting the absence of intra-chain disulphide bonds in their structure.
authors.affiliation: Sezione di S. Angelo Lodigiano, Istituto Sperimentale Cerealicoltura, via Mulino 3, 20079, S. Angelo Lodigiano, Milano, Italy.
authors.forename: T
authors.initials: T
authors.lastname: Dachkevitch
authors.forename: R
authors.initials: R
authors.lastname: Redaelli
authors.forename: A M
authors.initials: AM
authors.lastname: Biancardi
authors.forename: E V
authors.initials: EV
authors.lastname: Metakovsky
authors.forename: N E
authors.initials: NE
authors.lastname: Pogna