title: Garcinia vilersiana bark extract activates the Nrf2/HO-1 signaling pathway in RAW264.7 cells.
abstract: Garcinia vilersiana is a traditional medicinal plant in Vietnam. The petroleum ether extract of stem bark of Garcinia vilersiana (GVE) was prepared to evaluate its potential to activate Nrf2, a transcription factor of antioxidant and detoxifying enzymes. Exposure of mouse macrophage RAW264.7 cells to GVE (0.625-2.5 µg/ml) resulted in a significant activation of Nrf2, as evaluated by nuclear accumulation of this transcription factor, and increased antioxidant response element (ARE) binding activity in a time- and concentration-dependent manner. As a result, GVE caused ARE-dependent up-regulation of heme oxygenase-1 (HO-1) in the cells. These results suggest that GVE contains components that have the ability to activate the Nrf2/ARE/HO-1 signaling pathway, leading to cellular protection.
authors.affiliation: Doctoral Program in Biomedical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Shinkai
authors.forename: Ichiro
authors.initials: I
authors.lastname: Yamanaka
authors.forename: Ho Huynh Thuy
authors.initials: HH
authors.lastname: Duong
authors.forename: Nguyen Thi
authors.initials: NT
authors.lastname: Quynh
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Kanaho
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Kumagai
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000818:Animals; D061745:Antioxidant Response Elements / Q000502:physiology; D002478:Cells, Cultured; D019610:Cytoprotection / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D029760:Garcinia; D051547:Heme Oxygenase-1 / Q000378:metabolism* / Q000502:physiology; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D051267:NF-E2-Related Factor 2 / Q000378:metabolism* / Q000502:physiology; D024301:Plant Bark; D010936:Plant Extracts / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse; D010936:Plant Extracts; D051547:Heme Oxygenase-1