title: A potential role of aminoglycoside resistance in endemic occurrence of Pseudomonas aeruginosa strains in lower airways of mechanically ventilated patients.
abstract: Altogether, 98 Pseudomonas aeruginosa isolates from a 5-bed intensive care unit were fingerprinted with pulsed-field gel electrophoresis and tested for aminoglycoside resistance genes aac(6')-Ib, aac(3″)-IIa, ant(2″)-Ia, armA, rmtA, and rmtB and integrons and virulence genes/operons phzI, phzII, phzM, phzS, apr, lasB, plcH, plcN, pilA, algD, toxA, exoS, exoT, exoY, and exoU. Two major clusters were identified (49 and 19 isolates), harbouring aac(6')-Ib, blaPSE-1, and ant(3″)-Ia genes or ant(2″)-Ia gene, respectively, on a class I integron. Most virulence genes except for exoU and pilA were found. Only 1 isolate of the minor cluster (8 isolates) and 1 of the 22 sporadic isolates carried integrons (without gene cassettes); virulence profile was highly variable. Comparing the resistance and virulence patterns of endemic and sporadic isolates suggests that integron-borne aminoglycoside resistance is more closely associated with the frequency than virulence. Consequently, aminoglycoside usage may have played a role in maintenance of the endemic clones.
authors.affiliation: Department of Medical Microbiology, Medical and Health Science Center, University of Debrecen, H-4032 Debrecen Nagyerdei krt. 98, Hungary.
authors.forename: Julianna
authors.initials: J
authors.lastname: Mózes
authors.affiliation: Department of Pulmonology, Medical and Health Science Center, University of Debrecen, H-4032 Debrecen Nagyerdei krt. 98, Hungary.
authors.forename: Ildikó
authors.initials: I
authors.lastname: Szűcs
authors.affiliation: Department of Medical Microbiology, Medical and Health Science Center, University of Debrecen, H-4032 Debrecen Nagyerdei krt. 98, Hungary.
authors.forename: Dávid
authors.initials: D
authors.lastname: Molnár
authors.affiliation: Department of Medical Microbiology, Medical and Health Science Center, University of Debrecen, H-4032 Debrecen Nagyerdei krt. 98, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Jakab
authors.affiliation: Department of Medical Microbiology, Medical and Health Science Center, University of Debrecen, H-4032 Debrecen Nagyerdei krt. 98, Hungary.
authors.forename: Ebrahimi
authors.initials: E
authors.lastname: Fatemeh
authors.affiliation: Department of Pulmonology, Medical and Health Science Center, University of Debrecen, H-4032 Debrecen Nagyerdei krt. 98, Hungary.
authors.forename: Mária
authors.initials: M
authors.lastname: Szilasi
authors.affiliation: Department of Medical Microbiology, Medical and Health Science Center, University of Debrecen, H-4032 Debrecen Nagyerdei krt. 98, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Majoros
authors.affiliation: Department of Hospital Hygiene and Infection Control, Medical and Health Science Center, University of Debrecen, H-4032 Debrecen Nagyerdei krt. 98, Hungary.
authors.forename: Piroska
authors.initials: P
authors.lastname: Orosi
authors.affiliation: Department of Medical Microbiology, Medical and Health Science Center, University of Debrecen, H-4032 Debrecen Nagyerdei krt. 98, Hungary. Electronic address: kg@med.unideb.hu.
authors.forename: Gábor
authors.initials: G
authors.lastname: Kardos
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000617:Aminoglycosides / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004363:Drug Utilization; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D012121:Respiration, Artificial / Q000009:adverse effects*; D012137:Respiratory System / Q000382:microbiology*; D037521:Virulence Factors / Q000235:genetics; D055815:Young Adult
chemical_list: D000617:Aminoglycosides; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D037521:Virulence Factors
keywords: Amikacin; Gene cassette; Integron; Pulsed-field gel electrophoresis; Virulence factors