title: An innovative monolithic column preparation for the isolation of 25  kilo base pairs DNA.
abstract: The use of large DNAs in preparing multivalent vaccines that will eventually give protective immunity against multiple pathogenic microbes is becoming a major debate nowadays. One of the important issues in ensuring the successful implementation of the new vaccine technology is the development of a chromatographic technique that can handle larger DNAs. This paper reports the development of a novel conical monolithic column format with pore and surface characteristics engineered for the isolation of 25 kbp DNA in a single step fashion. An effective method of eliminating wall channelling, a defect of most conventional monolithic chromatography systems which has caused significant loss of product, was applied to maximise DNA recovery. This method was based on a systematic reduction of wall channel size based on a predetermined correlation between column’s back pressure and wall channel size of a particular monolith pore size.
authors.forename: Clarence M
authors.initials: CM
authors.lastname: Ongkudon
authors.forename: Sharadwata
authors.initials: S
authors.lastname: Pan
authors.forename: Michael K
authors.initials: MK
authors.lastname: Danquah
mesh_terms: D020029:Base Pairing; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D008970:Molecular Weight
chemical_list: D004269:DNA, Bacterial