title: Novel seed lipid phenotypes in combinations of mutants altered in fatty acid biosynthesis inArabidopsis.
abstract: The seed fatty acid (FA) composition of various single mutant combinations ofArabidopsis thaliana that affect FA biosynthesis has been examined. Double mutant combinations offae, a mutation affecting CIS elongation, and a series of four other FA biosynthetic mutants were synthesized. The four other single mutants were:fad2 andfad3, which are deficient in 18∶1 and 18∶2 desaturation, respectively;fab1, which is elevated in 16∶0 and decreased in 18∶1; andfab2, which is elevated in 18∶0 and decreased in 18∶1. The superimposition of two blocks in the FA biosynthetic pathway leads to dramatic changes in the FA content of the double mutants. The tenArabidopsis stocks analyzed to date (wild-type, five single mutants, and four double mutants) make seed oils with a wide range of FA compositions, and illustrate the diversity of oils it is possible to obtain from a single plant species.
authors.affiliation: DNA Plant Technology, 6701 San Pablo Ave., 94608, Oakland, CA, USA.
authors.forename: D W
authors.initials: DW
authors.lastname: James
authors.forename: H K
authors.initials: HK
authors.lastname: Dooner