title: Immunohistochemistry on freely floating fixed tissue sections.
abstract: Immunohistochemistry on free floating tissue sections is done for many reasons, all of which involve labeling tissue to visualize a certain cell type, protein, or structural component. Visualization is aided by mounting sections on microscope slides for stabilization, and is in most cases necessary for the appropriate use of objectives with a high numerical aperture and high degree of magnification.
authors.affiliation: Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: jbachma9@gmail.com.
authors.forename: Julia
authors.initials: J
authors.lastname: Bachman
mesh_terms: D000818:Animals; D000906:Antibodies; D004210:Dissection / Q000379:methods; D007150:Immunohistochemistry / Q000295:instrumentation / Q000379:methods*; D008853:Microscopy / Q000295:instrumentation / Q000379:methods; D016707:Tissue Fixation / Q000379:methods
chemical_list: D000906:Antibodies
keywords: Blocking nonspecific binding; Immunohistochemistry; Mount labeled tissue section; Pretreatment of tissue section; Primary antibody labeling; Secondary antibody labeling