title: Energetic contributions to channel gating of residues in the muscle nicotinic receptor β1 subunit.
abstract: In the pentameric ligand-gated ion channel family, transmitter binds in the extracellular domain and conformational changes result in channel opening in the transmembrane domain. In the muscle nicotinic receptor and other heteromeric members of the family one subunit does not contribute to the canonical agonist binding site for transmitter. A fundamental question is whether conformational changes occur in this subunit. We used records of single channel activity and rate-equilibrium free energy relationships to examine the β1 (non-ACh-binding) subunit of the muscle nicotinic receptor. Mutations to residues in the extracellular domain have minimal effects on the gating equilibrium constant. Positions in the channel lining (M2 transmembrane) domain contribute strongly and relatively late during gating. Positions thought to be important in other subunits in coupling the transmitter-binding to the channel domains have minimal effects on gating. We conclude that the conformational changes involved in channel gating propagate from the binding-site to the channel in the ACh-binding subunits and subsequently spread to the non-binding subunit.
authors.affiliation: Department of Anesthesiology and the Taylor Family Institute for Innovative Psychiatric Research, Washington University School of Medicine, Saint Louis, Missouri, United States of America.
authors.forename: Gustav
authors.initials: G
authors.lastname: Akk
authors.forename: Megan
authors.initials: M
authors.lastname: Eaton
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Steven
authors.initials: S
authors.lastname: Zheng
authors.forename: Joshua
authors.initials: J
authors.lastname: Lo
authors.forename: Joe Henry
authors.initials: JH
authors.lastname: Steinbach
mesh_terms: D000818:Animals; D004734:Energy Metabolism / Q000502:physiology; D057809:HEK293 Cells; D006801:Humans; D015640:Ion Channel Gating / Q000502:physiology*; D051379:Mice; D008958:Models, Molecular; D018482:Muscle, Skeletal / Q000378:metabolism / Q000502:physiology*; D016296:Mutagenesis; D011487:Protein Conformation; D011978:Receptors, Nicotinic / Q000378:metabolism*
chemical_list: C495964:Chrnb1 protein, mouse; D011978:Receptors, Nicotinic