title: Characterization of antibacterial activity of bikaverin from Fusarium sp. HKF15.
abstract: Redeemed interest in discovery and characterization of naturally occurring antimicrobials arises from the fact that these compounds possess diverse biological activities useful in the development of new therapeutics. In this study, 35 fungi from extreme environments of effluent treatment plants were screened for antimicrobial activity. An isolate HKF15 produced antimicrobial compound that showed activities against pathogenic and multidrug resistant bacteria; and was identified as Fusarium sp. The active principle was separated through activity-guided HPLC fractionation of 1:1 ethyl acetate crude extract that was identified as bikaverin using HRMS and (1)H NMR. Bikaverin is known for antitumor and antifungal activity and in this study; antibacterial activity of bikaverin was assessed, emphasizing the importance of repurposing of antibiotics.
authors.affiliation: CSIR - National Environmental Engineering Research Institute, Nehru Marg, Nagpur 440020, Maharashtra, India.
authors.forename: Radhika
authors.initials: R
authors.lastname: Deshmukh
authors.affiliation: Piramal Enterprises Ltd., 1, Nirlon Complex, Off Western Express Highway, Goregaon (East), Mumbai 400 063, India.
authors.forename: Anoop
authors.initials: A
authors.lastname: Mathew
authors.affiliation: CSIR - National Environmental Engineering Research Institute, Nehru Marg, Nagpur 440020, Maharashtra, India. Electronic address: hemantdrd@hotmail.com.
authors.forename: Hemant J
authors.initials: HJ
authors.lastname: Purohit
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D000935:Antifungal Agents / Q000302:isolation & purification; D000970:Antineoplastic Agents / Q000302:isolation & purification; D001419:Bacteria / Q000187:drug effects; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D004353:Drug Evaluation, Preclinical; D024901:Drug Resistance, Multiple, Bacterial; D005658:Fungi / Q000737:chemistry / Q000302:isolation & purification; D005670:Fusarium / Q000737:chemistry* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D044004:Xanthones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000970:Antineoplastic Agents; D044004:Xanthones; C000269:bikaverin
keywords: 18S rRNA gene; Antimicrobial activity; Bikaverin; Effluent treatment plant; Fusarium