title: Immunological studies on chlorophyll-a/b proteins and their distribution in thylakoid membrane domains.
abstract: The immunological relationships between chlorophyll-a/b proteins from higher-plant thylakoid membranes have been studied by assaying purified chlorophyll proteins (CPs) with polyclonal and monoclonal antibodies. Although low levels of cross-reactions were observed between all light-harvesting proteins, the peripheral antennae (LHCII) were largely distinct from the inner antennae (CP 26 and CP 29). Chlorophyll-protein 24 and LHCI-680 have been proposed to have a role in connecting the inner and outer antennae, respectively, in photosystems I and II, and were closely related. The immunological relationships closely corresponded to the spectral properties. Antibodies were also used for locating chlorophyll-a/b proteins in grana, stroma and bundle-sheath membranes showing a strong lateral heterogeneity, which was maintained following State I State II transition. The only exception to this pattern was a specific LHCII population enriched in State-II stroma membranes. Chlorophyll proteins from bundlesheath chloroplasts, that have only cyclic electron flow, had epitopes distinct from those of their mesophyll homologues.
authors.affiliation: Dipartimento di Biologia, Università di Padova, Via Trieste 75, I-35121, Padova, Italy.
authors.forename: M L
authors.initials: ML
authors.lastname: Di Paolo
authors.forename: A
authors.initials: A
authors.lastname: Dal Belin Peruffo
authors.forename: R
authors.initials: R
authors.lastname: Bassi