title: Agrobacterium tumefaciens-mediated transformation of safflower (Carthamus tinctorius L.) cv. 'Centennial'.
abstract: Efficient callus formation was achieved from cotyledon, stem, and leaf expiants of the domestic safflower cultivar 'Centennial' on MS salts medium containing 1 mg/L BAP and 1 mg/L NAA. Shoot buds were regenerated from 26% of leaf-derived calli on callus induction medium, although attempts to root regenerated shoots were not successful. 'Centennial' expiants inoculated with Agrobacterium tumefaciens containing NPT II and GUS genes produced kanamycin-resistant calli from which buds were regenerated. Transformation and stable integration of transgenes was confirmed by GUS assay and DNA hybridization in kanamycin-resistant calli, and GUS assay in regenerated shoots.
authors.affiliation: Plant and Soil Science Department, Montana State University, 59717, Bozeman, Montana, USA.
authors.forename: M
authors.initials: M
authors.lastname: Ying
authors.forename: W E
authors.initials: WE
authors.lastname: Dyer
authors.forename: J W
authors.initials: JW
authors.lastname: Bergman