title: [Comparative study on hepatic toxicity of gardeniae fructus and Huanglian Jiedu decoction].
abstract: OBJECTIVE
  To observe the effect of ingredients in Huanglian Jiedu decoction (HLJDT) combined with Gardeniae Fructus on the hepatic toxicity of Gardeniae Fructus and its mechanism.
  
  
  METHODS
  Rats were given Gardeniae Fructus and HLJDT decoction at the dose of 10 times of clinical dosage for 3 days. Their ALT AST, ALP, TBA were detected, and their liver weight index was calculated. SOD activity, MDA content, GSH-PX activity, TNF-alpha content in hepatic tissues were determined. The cell apoptosis in liver tissue was determined by TUNEL, and the expressions of apoptosis related proteins Bax, Bcl-2 were measured by immunohistochemical method.
  
  
  RESULTS
  Compared with the normal control group, the Gardeniae Fructus group showed significant increase in the liver weight index, ALT, AST, TBA and ALP, notable decrease in SOD, SOD/MDA and GSH-PX, and remarkable rise in MDA, TNF-a concentration, accumulated optical density, apoptosis index, Bax and Bax/Bcl-2. Compare with that in the Gardeniae Fructus group, the liver index, ALT, AST, TBA, ALP reduced obviously; SOD, SOD/MDA and GSH-PX markedly increased; MDA and TNF-alpha significantly reduced; the accumulated optical density and apoptosis index significantly reduced; and Bax/Bcl-2 was much lower in HLJDT group.
  
  
  CONCLUSIONS
  The hepatic toxicity caused by Gardeniae Fructus may be related to inflammation, oxidative stress-induced hepatocyte necrosis and apoptosis. Other ingredients in HLJDT, apart from Gardeniae Fructus, can decrease the hepatic toxicity caused by Gardeniae Fructus by increasing the enzyme activity eliminating radicals and inhibiting hepatocyte injury caused by inflammatory reaction against Gardeniae Fructus.
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. wangkun200711@163.com
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Ruo-Min
authors.initials: RM
authors.lastname: Jin
authors.forename: Chang-Xun
authors.initials: CX
authors.lastname: Chen
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000633:toxicity*; D032062:Gardenia / Q000737:chemistry* / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004365:Drugs, Chinese Herbal; C494417:huanglian