title: Effects of bovine colostrum supplementation on upper respiratory illness in active males.
abstract: Bovine colostrum (COL) has been advocated as a nutritional countermeasure to exercise-induced immune dysfunction and increased risk of upper respiratory illness (URI) in athletic populations, however, the mechanisms remain unclear. During winter months, under double-blind procedures, 53 males (mean training load±SD, 50.5±28.9 MET-hweek(-1)) were randomized to daily supplementation of 20g of COL (N=25) or an isoenergetic/isomacronutrient placebo (PLA) (N=28) for 12weeks. Venous blood was collected at baseline and at 12weeks and unstimulated saliva samples at 4 weeks intervals. There was a significantly lower proportion of URI days and number of URI episodes with COL compared to PLA over the 12weeks (p<0.05). There was no effect of COL on in vitro neutrophil oxidative burst, salivary secretory IgA or salivary antimicrobial peptides (p>0.05), which does not support previously suggested mechanisms. In a subset of participants (COL=14, PLA=17), real-time quantitative PCR, targeting the 16S rRNA gene showed there was an increase in salivary bacterial load over the 12 weeks period with PLA (p<0.05) which was not as evident with COL. Discriminant function analysis of outputs received from serum metabolomics showed changes across time but not between groups. This is the first study to demonstrate that COL limits the increased salivary bacterial load in physically active males during the winter months which may provide a novel mechanism of immune-modulation with COL and a relevant marker of in vivo (innate) immunity and risk of URI.
authors.affiliation: Department of Sport and Exercise Science, Aberystwyth University, Aberystwyth, UK; Clinical Research Centre, Prince Phillip Hospital, Llanelli, UK. Electronic address: awj7@aber.ac.uk.
authors.forename: Arwel W
authors.initials: AW
authors.lastname: Jones
authors.affiliation: Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, UK.
authors.forename: Simon J S
authors.initials: SJ
authors.lastname: Cameron
authors.affiliation: Department of Sport and Exercise Science, Aberystwyth University, Aberystwyth, UK.
authors.forename: Rhys
authors.initials: R
authors.lastname: Thatcher
authors.affiliation: Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, UK.
authors.forename: Marikka S
authors.initials: MS
authors.lastname: Beecroft
authors.affiliation: Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, UK.
authors.forename: Luis A J
authors.initials: LA
authors.lastname: Mur
authors.affiliation: Endurance Research Group, School of Sport and Exercise Sciences, University of Kent at Medway, Chatham, UK.
authors.forename: Glen
authors.initials: G
authors.lastname: Davison
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D002417:Cattle; D003126:Colostrum / Q000276:immunology*; D004311:Double-Blind Method; D015444:Exercise / Q000502:physiology*; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000378:metabolism; D008297:Male; D009504:Neutrophils / Q000502:physiology; D016897:Respiratory Burst; D012141:Respiratory Tract Infections / Q000188:drug therapy / Q000517:prevention & control*
chemical_list: D000890:Anti-Infective Agents; D007071:Immunoglobulin A, Secretory
keywords: 16S rRNA; Innate immunity; Metabolomics; Microbiome; Mucosal immunity; URTI