title: Molecular detection and partial characterization of Tomato Yellow Leaf Curl Virus in Sri Lanka.
abstract: Tomato Yellow Leaf Curl Virus (TYLCV) is an important plant virus on one of the economically most important vegetable crops; tomato (Lycopersicon esculentum Mill.). This had not been molecularly detected before, in Sri Lanka. TYLCV-GN-SL was isolated from apparently infected tomato plants using modified Cetyltrimethyl Ammonium Bromide (CTAB) method in Gannoruwa. Associated Begomoviruses were detected using Deng 541/Deng 540 and AV 494/AC 1048 primer pairs. TYLCV was detected for the first time in tomato in Sri Lanka using P1V/P4C, TYLCV specific primer pair. Nucleotide sequence of coat protein of isolated TYLCV-GN-SL proved that the Indian strain of ToLC virus was closely related to Tomato Leaf Curl Sri Lanka Virus (TLCV-SL: 97%) and Tomato leaf curl Geminivirus (TLCGV: 93%) through direct sequencing data. TLCV-SL was confirmed as TYLCV isolate. TYLCV was molecularly detected from major tomato growing districts like Badulla, Nuwara-Eliya, Kandy and Matale in Sri Lanka.
authors.affiliation: Faculty of Agriculture, Rajarata University of Sri Lanka, Puliyankulama, Anuradhapura, Sri Lanka.
authors.forename: S A M C
authors.initials: SA
authors.lastname: Samarakoon
authors.forename: A
authors.initials: A
authors.lastname: Balasuriya
authors.forename: R G A S
authors.initials: RG
authors.lastname: Rajapaksha
authors.forename: W A R T
authors.initials: WA
authors.lastname: Wickramarachchi
mesh_terms: D001483:Base Sequence; D053839:Begomovirus / Q000145:classification / Q000235:genetics*; D017931:DNA Primers; D005814:Genes, Viral; D018551:Solanum lycopersicum / Q000821:virology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013188:Sri Lanka
chemical_list: D017931:DNA Primers