title: Effect of nurse cultures on the production of macro-calli and fertile plants from maize embryogenic suspension culture protoplasts.
abstract: Fertile plants have been obtained from maize (Zea mays L.) embryogenic suspension culture protoplasts. Friable, embryogenic callus initiated from an immature embryo from a cross involving the genotypes A188, B73, and Black Mexican sweetcorn was used to establish a rapidly growing embryogenic suspension culture. After nine months in culture, high yields of viable protoplasts (30×10(6)/ gram fresh weight) were obtained following a 1.5 hour enzymatic digestion. Protoplasts cultured with feeder cells divided and formed embryogenic callus, from which male and female fertile plants were regenerated. Protoplast-derived R1 plants were self-pollinated and immature R2 embryos isolated for callus initiation. Female fertile plants have also been produced from protoplasts isolated from an R2-derived suspension culture. Significant interactions between protoplast and feeder-cell lines were observed.
authors.affiliation: Monsanto Agricultural Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO., USA.
authors.forename: W L
authors.initials: WL
authors.lastname: Petersen
authors.forename: S
authors.initials: S
authors.lastname: Sulc
authors.forename: C L
authors.initials: CL
authors.lastname: Armstrong