title: Computational designing of a poly-epitope fecundity vaccine for multiple species of livestock.
abstract: Inhibin and follistatin are known to reduce fecundity by inhibiting the actions of activin and FSH. Thus, the immunoneutralization of these hormones is a rational proposal for augmenting reproductive performance. The present study describes a comprehensive computational methodology comprising of a consensus approach of several B- and Th-cell epitope prediction tools for the identification of epitopic regions within the structure of these hormones that can be incorporated into a poly-epitope fecundity vaccine. The proposed peptide (RGD-WSPAALRLLQRPPEEPA-KK-YSFPISSILE) should be effective in multiple animal species, generating good immunological memory.
authors.affiliation: Animal Biotechnology Center, Department of Veterinary Physiology and Biochemistry, College of Veterinary and Animal Sciences, G. B. Pant University of Agriculture and Technology, Pantnagar 263145, Uttarakhand, India. Electronic address: vetbhaskar@gmail.com.
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Ganguly
authors.affiliation: Animal Biotechnology Center, Department of Veterinary Physiology and Biochemistry, College of Veterinary and Animal Sciences, G. B. Pant University of Agriculture and Technology, Pantnagar 263145, Uttarakhand, India.
authors.forename: Sunil Kumar
authors.initials: SK
authors.lastname: Rastogi
authors.affiliation: Department of Animal Reproduction, Gynecology and Obstetrics, College of Veterinary and Animal Sciences, G. B. Pant University of Agriculture and Technology, Pantnagar 263145, Uttarakhand, India.
authors.forename: Shiv
authors.initials: S
authors.lastname: Prasad
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002645:Chickens; D018604:Epitope Mapping / Q000379:methods; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry / Q000276:immunology; D005298:Fertility / Q000276:immunology*; D038681:Follistatin / Q000737:chemistry / Q000276:immunology; D006736:Horses; D007265:Inhibins / Q000737:chemistry / Q000276:immunology; D058751:Livestock / Q000276:immunology*; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D011487:Protein Conformation; D051381:Rats; D012756:Sheep; D034421:Sus scrofa; D014612:Vaccines / Q000276:immunology*; D022223:Vaccines, Subunit / Q000737:chemistry / Q000276:immunology
chemical_list: D000939:Epitopes; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D038681:Follistatin; D010455:Peptides; D014612:Vaccines; D022223:Vaccines, Subunit; D007265:Inhibins
keywords: Agretope; B-cell; Epitope; Fecundity; Follistatin; Helper T-cell; Inhibin; Peptide; Vaccine; Veterinary