title: Probing subcellular organic hydroperoxide formation via a genetically encoded ratiometric and reversible fluorescent indicator.
abstract: A ratiometric and reversible organic hydroperoxide (OHP) sensor, rOHSer, was developed with high sensitivity and selectivity for subcellular OHP visualization. Through targeting rOHSer to the nucleus, we demonstrated that high levels of D-glucose cause elevated OHP production in this compartment. Further utilization of rOHSer probe may allow more elucidation of unique roles of OHPs in diverse biological processes.
authors.affiliation: Synthetic and Functional Biomolecules Center (SFBC), Beijing National Laboratory for Molecular Sciences (BNLMS), Department of Chemical Biology, College of Chemistry, Peking University, Beijing, 100871, China. pengchen@pku.edu.cn.
authors.forename: Boxuan Simen
authors.initials: BS
authors.lastname: Zhao
authors.forename: Gong
authors.initials: G
authors.lastname: Zhang
authors.forename: Shizhe
authors.initials: S
authors.lastname: Zeng
authors.forename: Chuan
authors.initials: C
authors.lastname: He
authors.forename: Peng R
authors.initials: PR
authors.lastname: Chen
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D005947:Glucose / Q000032:analysis; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D018613:Microscopy, Confocal; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D059008:Time-Lapse Imaging; D020122:tert-Butylhydroperoxide / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins; C054562:yellow fluorescent protein, Bacteria; D020122:tert-Butylhydroperoxide; D005947:Glucose