title: SIRT1 negatively regulates the protein stability of HIPK2.
abstract: In the present study, we investigated whether a histone deacetylase sirtuin 1 (SIRT1) can regulate the protein stability of homeodomain-interacting protein kinase 2 (HIPK2). We observed the evidence of molecular interaction between SIRT1 and HIPK2. Interestingly, overexpression or pharmacological activation of SIRT1 promoted ubiquitination and the proteasomal degradation of HIPK2 whereas inhibition of SIRT1 activity increased the protein level of HIPK2. Furthermore, a SIRT1 activator decreased the level of HIPK2 acetylation whereas an inhibitor increased the acetylation level. These results suggest that SIRT1 may deacetylate and promote the ubiquitination and subsequent proteasomal degradation of HIPK2.
authors.affiliation: Department of Molecular Biology, Sejong University, Seoul 143-747, Republic of Korea.
authors.forename: Joohyun
authors.initials: J
authors.lastname: Hwang
authors.forename: Seo-Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Jong-Ryoul
authors.initials: JR
authors.lastname: Choi
authors.forename: Ki Soon
authors.initials: KS
authors.lastname: Shin
authors.forename: Cheol Yong
authors.initials: CY
authors.lastname: Choi
authors.forename: Shin Jung
authors.initials: SJ
authors.lastname: Kang
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D055550:Protein Stability; D059748:Proteolysis; D056564:Sirtuin 1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D002352:Carrier Proteins; C115114:HIPK2 protein, human; D017346:Protein Serine-Threonine Kinases; D046988:Proteasome Endopeptidase Complex; C447939:SIRT1 protein, human; D056564:Sirtuin 1
keywords: Deacetylation; HIPK2; Proteasomal degradation; SIRT1; Ubiquitination