title: The characteristics of myosin heavy chain-based fiber types in porcine longissimus dorsi muscle.
abstract: The aim of this study is to type fibers from porcine longissimus dorsi (LD) muscles according to their distribution of myosin heavy chain (MHC) isoforms as well as to investigate fiber characteristics. Four pure types, including types I, IIA, IIX, and IIB were labeled and two hybrid fiber types were subdivided into type IIAX and IIXB by immunohistochemistry using four monoclonal antibodies. Porcine LD muscles were found to have 92.79 (number) and 92.10% (area) of pure type composition, while the composition of hybrid fibers was 8.22 (number) and 9.71% (area). The activities of myosin ATPase increased in the following order: type I, IIAX, IIA = IIX = IIB and IIXB. The succinate dehydrogenase and glycerol-3-phosphate dehydrogenase activities were higher in fiber types I and IIB, respectively. The characteristics of hybrid fibers were observed that their characteristics did not lie between the properties for their respective pure phenotypes.
authors.affiliation: Division of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju 690-701, Republic of Korea.
authors.forename: Gap-Don
authors.initials: GD
authors.lastname: Kim
authors.forename: Youn-Chul
authors.initials: YC
authors.lastname: Ryu
authors.forename: Cheorun
authors.initials: C
authors.lastname: Jo
authors.forename: Jung-Gyu
authors.initials: JG
authors.lastname: Lee
authors.forename: Han-Sul
authors.initials: HS
authors.lastname: Yang
authors.forename: Jin-Yeon
authors.initials: JY
authors.lastname: Jeong
authors.forename: Seon-Tea
authors.initials: ST
authors.lastname: Joo
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D018485:Muscle Fibers, Skeletal / Q000737:chemistry*; D018995:Myosin Heavy Chains / Q000737:chemistry*; D020033:Protein Isoforms / Q000737:chemistry; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D020033:Protein Isoforms; D005993:Glycerolphosphate Dehydrogenase; C471850:glycerol-3-phosphate dehydrogenase, FAD-dependent; D018995:Myosin Heavy Chains
keywords: Hybrid fiber; Longissimus dorsi; Muscle fiber; Myosin heavy chain; Pig