title: Plant regeneration from protoplasts of durum wheat (Triticum durum Desf. cv. D6962).
abstract: Suspension cultures of durum wheat were established from embryogenic callus maintained in liquid medium for 30 months. Protoplasts were readily isolated from the suspension cultures with yields as high as 3 X 10(7) protoplasts per g fresh weight suspension cells. When incubated in a modified MS medium containing half strength of the macroelements, 5 μM 2,4-D (2,4-dichlorophenoxyacetic acid) and 0.6 M glucose, protoplast-derived cells divided at frequencies ranging from 1.4 to 10.0 %. After transfer to a solid subculture medium, the protoplast-derived colonies formed embryogenic protuberances, from which green plants have been regenerated.
authors.affiliation: Department of Biochemistry, The University of Queensland, 4072, Brisbane, Australia.
authors.forename: Y M
authors.initials: YM
authors.lastname: Yang
authors.forename: D D
authors.initials: DD
authors.lastname: He
authors.forename: K J
authors.initials: KJ
authors.lastname: Scott