title: Clinical significance of autoantibodies to the pericentromeric heterochromatin protein 1a protein.
abstract: OBJECTIVE
  The objective of the study is to determine the frequency and the clinical significance of autoantibodies to the pericentromeric heterochromatin protein 1 (HP1). So far this antinuclear antibody specificity has been mainly reported in patients with the CREST syndrome.
  
  
  METHODS
  We screened the sera of 199 individuals, including patients suffering from various autoimmune disorders (Group I, n=145) and non autoimmune diseases (Group II, n=44 patients) as well as healthy individuals (Group III, n=30). The sera were systematically tested by Western blot and ELISA using a GST-HP1α fusion protein as an antigen.
  
  
  RESULTS
  Anti-HP1 antibodies were detected in 32% of patients in Group I, 11.3% in Group II and 3.3% of individuals in Group III. They could be detected in sera containing or not antinuclear antibodies detectable by indirect immunofluorescence. Anti-HP1 antibodies were mostly associated with the CREST and Sjogren's syndromes (70% and 44.4%, respectively). They could also be detected in 22.2% of patients suffering from various other autoimmune diseases. However, their negative predictive value was 94% in the CREST syndrome.
  
  
  CONCLUSIONS
  Anti-HP1 autoantibodies are associated with a large spectrum of disorders. However, they have a diagnostic value in the CREST syndrome.
authors.affiliation: Département d'Hématologie, Hôpital Saint-Antoine, Centre de Référence des Microangiopathies thrombotiques, France; Hôpital Saint Antoine, AP-HP, Université Pierre et Marie Curie (UPMC), Paris, France; Unité Inserm U1009, Institut Gustave Roussy, Villejuif, France.
authors.forename: Paul
authors.initials: P
authors.lastname: Coppo
authors.forename: Isabelle
authors.initials: I
authors.lastname: Henry-Dessailly
authors.forename: Jacques
authors.initials: J
authors.lastname: Rochette
authors.forename: Catherine
authors.initials: C
authors.lastname: Lok
authors.forename: Brigitte
authors.initials: B
authors.lastname: Buendia
authors.forename: Kaiss
authors.initials: K
authors.lastname: Lassoued
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000974:Antibodies, Antinuclear / Q000276:immunology*; D016736:Antiphospholipid Syndrome / Q000175:diagnosis / Q000276:immunology; D001172:Arthritis, Rheumatoid / Q000175:diagnosis / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology; D001327:Autoimmune Diseases / Q000175:diagnosis / Q000276:immunology; D017675:CREST Syndrome / Q000175:diagnosis / Q000276:immunology*; D016022:Case-Control Studies; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012595:Scleroderma, Systemic / Q000175:diagnosis / Q000276:immunology; D012859:Sjogren's Syndrome / Q000175:diagnosis / Q000276:immunology*; D055815:Young Adult
chemical_list: D000974:Antibodies, Antinuclear; D001323:Autoantibodies; C000715594:CBX5 protein, human; D002868:Chromosomal Proteins, Non-Histone; C538950:anticentromere antibody; D000090266:Chromobox Protein Homolog 5
keywords: Anti-centromere antibodies; Antinuclear antibodies; Autoimmune disorders; CREST; HP1; Systemic sclerosis