title: Frequency of G2677T/A and C3435T polymorphisms of MDR1 gene in preeclamptic women.
abstract: OBJECTIVE
  Preeclampsia (PE) belongs to main causes of mortality rates of mothers, fetuses and new born children. Polymorphism of MDR1 gene is connected with reduction of P-glycoprotein expression in placenta and increased fetal exposure to xenobiotics. The aim of the study was to determine the frequency of C3435T and G2677T/A polymorphisms of MDR1 gene in pregnant women with preeclampsia.
  
  
  METHODS
  The study consisted of 180 Polish women including 60 women with PE and 120 healthy pregnant women. Determination of C3435T and G2677T/A polymorphisms of MDR1 gene was performed using PCR-RFLP method.
  
  
  RESULTS
  No significant association between genotypes of the examined polymorphisms and the clinical parameters of pregnant women with PE was observed However the interesting tendency to higher prevalence of mutated 2677A allele of G2677T/A MDR1 polymorphism in PE group has been shown (2,50 vs. 0,83% in controls, OR=3,05, ns).
  
  
  CONCLUSIONS
  The results of this study suggest no significant effect of examined C3435T and G2677T/A MDR1 polymorphisms in PE pathogenesis. However given the noteworthy results related to mutated 2677A allele of G2677T/A MDR1 polymorphism in preeclamptic women further studies seem to be needed. Nevertheless, the frequency of investigated polymorphisms was consistent with the distribution in other Caucasian populations.
authors.affiliation: Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, University of Medical Sciences, Poznan, Poland. aniabogacz23@o2.pl
authors.forename: Anna
authors.initials: A
authors.lastname: Bogacz
authors.forename: Przemysław M
authors.initials: PM
authors.lastname: Mrozikiewicz
authors.forename: Donata
authors.initials: D
authors.lastname: Deka-Pawlik
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Seremak-Mrozikiewicz
authors.forename: Joanna
authors.initials: J
authors.lastname: Bartkowiak-Wieczorek
authors.forename: Magdalena
authors.initials: M
authors.lastname: Barlik
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Drews
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Kowalska
authors.forename: Edmund
authors.initials: E
authors.lastname: Grześkowiak
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics*; D000328:Adult; D005260:Female; D005787:Gene Frequency; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011225:Pre-Eclampsia / Q000235:genetics* / Q000378:metabolism; D011247:Pregnancy; D012307:Risk Factors; D044465:White People / Q000235:genetics*; D055815:Young Adult
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1