title: Easy determination of ploidy level in Arabidopsis thaliana plants by means of pollen size measurement.
abstract: Cytogenetic examination of transgenic Arabidopsis thaliana (L.) Heynh. plants obtained by Agrobacterium-mediated gene transfer to cotyledon- and root-explants or by direct gene transfer into protoplasts revealed a high percentage of tetraploid or aneuploid transformants. Depending on the transformation procedure used, 13% (root explant transformation), 33% (cotyledon explant transformation), or 38% (direct gene transfer) of the transformants showed aberrant ploidy levels. A good correlation between the ploidy level of a plant and the size of its pollen grains was observed. This allows quick and simple testing of the ploidy level of transgenic Arabidopsis plants.
authors.affiliation: Institut für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, 14195, Berlin, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Altmann
authors.forename: B
authors.initials: B
authors.lastname: Damm
authors.forename: W B
authors.initials: WB
authors.lastname: Frommer
authors.forename: T
authors.initials: T
authors.lastname: Martin
authors.forename: P C
authors.initials: PC
authors.lastname: Morris
authors.forename: D
authors.initials: D
authors.lastname: Schweizer
authors.forename: L
authors.initials: L
authors.lastname: Willmitzer
authors.forename: R
authors.initials: R
authors.lastname: Schmidt