title: Phosphate transporter mediated lipid accumulation in Saccharomyces cerevisiae under phosphate starvation conditions.
abstract: In the current study, when phosphate transporters pho88 and pho86 were knocked out they resulted in significant accumulation (84% and 43%) of triacylglycerol (TAG) during phosphate starvation. However in the presence of phosphate, TAG accumulation was only around 45% in both pho88 and pho86 mutant cells. These observations were confirmed by radio-labeling, fluorescent microscope and RT-PCR studies. The TAG synthesizing genes encoding for acyltransferases namely LRO1 and DGA1 were up regulated. This is the first report for accumulation of TAG in pho88Δ and pho86Δ cells under phosphate starvation conditions.
authors.affiliation: Biomembrane Laboratory, Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
authors.forename: Antoni W
authors.initials: AW
authors.lastname: James
authors.forename: Vasanthi
authors.initials: V
authors.lastname: Nachiappan
mesh_terms: D000217:Acyltransferases / Q000378:metabolism; D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism*; D056804:Biofuels / Q000382:microbiology; D018533:Biomass; D002855:Chromatography, Thin Layer; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects / Q000235:genetics; D008055:Lipids / Q000302:isolation & purification; D050296:Microbial Viability / Q000187:drug effects; D009154:Mutation / Q000235:genetics; D028061:Phosphate Transport Proteins / Q000378:metabolism*; D010710:Phosphates / Q000172:deficiency* / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D056804:Biofuels; D008055:Lipids; C099982:PHO86 protein, S cerevisiae; C100799:PHO88 protein, S cerevisiae; D028061:Phosphate Transport Proteins; D010710:Phosphates; D029701:Saccharomyces cerevisiae Proteins; D000217:Acyltransferases
keywords: Acyltransferase; Biodiesel; Phosphate transporter; Saccharomyces cerevisiae; Triacylglycerol