title: Role of the Trypanosoma brucei HEN1 family methyltransferase in small interfering RNA modification.
abstract: Parasitic protozoa of the flagellate order Kinetoplastida represent one of the deepest branches of the eukaryotic tree. Among this group of organisms, the mechanism of RNA interference (RNAi) has been investigated in Trypanosoma brucei and to a lesser degree in Leishmania (Viannia) spp. The pathway is triggered by long double-stranded RNA (dsRNA) and in T. brucei requires a set of five core genes, including a single Argonaute (AGO) protein, T. brucei AGO1 (TbAGO1). The five genes are conserved in Leishmania (Viannia) spp. but are absent in other major kinetoplastid species, such as Trypanosoma cruzi and Leishmania major. In T. brucei small interfering RNAs (siRNAs) are methylated at the 3' end, whereas Leishmania (Viannia) sp. siRNAs are not. Here we report that T. brucei HEN1, an ortholog of the metazoan HEN1 2'-O-methyltransferases, is required for methylation of siRNAs. Loss of TbHEN1 causes a reduction in the length of siRNAs. The shorter siRNAs in hen1(-/-) parasites are single stranded and associated with TbAGO1, and a subset carry a nontemplated uridine at the 3' end. These findings support a model wherein TbHEN1 methylates siRNA 3' ends after they are loaded into TbAGO1 and this methylation protects siRNAs from uridylation and 3' trimming. Moreover, expression of TbHEN1 in Leishmania (Viannia) panamensis did not result in siRNA 3' end methylation, further emphasizing mechanistic differences in the trypanosome and Leishmania RNAi mechanisms.
authors.affiliation: Department of Internal Medicine, School of Medicine, Yale University, New Haven, Connecticut, USA.
authors.forename: Huafang
authors.initials: H
authors.lastname: Shi
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Barnes
authors.forename: Nicholas
authors.initials: N
authors.lastname: Carriero
authors.forename: Vanessa D
authors.initials: VD
authors.lastname: Atayde
authors.forename: Christian
authors.initials: C
authors.lastname: Tschudi
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Ullu
mesh_terms: D000595:Amino Acid Sequence; D007891:Leishmania / Q000235:genetics / Q000378:metabolism; D008780:Methyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D016053:RNA, Protozoan / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000201:enzymology / Q000378:metabolism*
chemical_list: D015800:Protozoan Proteins; D016053:RNA, Protozoan; D034741:RNA, Small Interfering; D008780:Methyltransferases