title: Lipid directed intrinsic membrane protein segregation.
abstract: We demonstrate a new approach for direct reconstitution of membrane proteins during giant vesicle formation. We show that it is straightforward to create a tissue-like giant vesicle film swelled with membrane protein using aquaporin SoPIP2;1 as an illustration. These vesicles can also be easily harvested for individual study. By controlling the lipid composition we are able to direct the aquaporin into specific immiscible liquid domains in giant vesicles. The oligomeric α-helical protein cosegregates with the cholesterol-poor domains in phase separating ternary mixtures.
authors.affiliation: Mork Family Department of Chemical Engineering and Materials Science, University of Southern California , 925 Bloom Walk, Los Angeles, California 90089, United States.
authors.forename: Jesper S
authors.initials: JS
authors.lastname: Hansen
authors.forename: James R
authors.initials: JR
authors.lastname: Thompson
authors.forename: Claus
authors.initials: C
authors.lastname: Hélix-Nielsen
authors.forename: Noah
authors.initials: N
authors.lastname: Malmstadt
mesh_terms: D008055:Lipids / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D010316:Particle Size; D013499:Surface Properties
chemical_list: D008055:Lipids; D008565:Membrane Proteins