title: Bacillus thuringiensis subsp. sichuansis strain MC28 produces a novel crystal protein with activity against Culex quinquefasciatus larvae.
abstract: The Bacillus thuringiensis subsp. sichuansis MC28 strain produces spherical parasporal crystals during sporulation and exhibits remarkable insecticidal activity against dipteran and lepidopteran pests. We characterized a novel cry gene (cry69Aa1), which was found in the pMC95 plasmid of the MC28 strain. The cry69Aa1 gene was inserted into a shuttle vector (pSTK) and expressed in an acrystalliferous mutant B. thuringiensis HD73⁻. In this transformant, a large number of spherical parasporal crystals, which were toxic to Culex quinquefasciatus (Diptera), were formed.
authors.affiliation: State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Guan
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Dai
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
authors.forename: Qiao
authors.initials: Q
authors.lastname: Li
authors.forename: Shuangcheng
authors.initials: S
authors.lastname: Li
authors.forename: Shiquan
authors.initials: S
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Aiping
authors.initials: A
authors.lastname: Zheng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology*; D003001:Cloning, Molecular; D003465:Culex / Q000187:drug effects* / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004731:Endotoxins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology*; D015870:Gene Expression; D005798:Genes, Bacterial; D005822:Genetic Vectors; D006460:Hemolysin Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology*; D007306:Insecticides / Q000302:isolation & purification* / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000502:physiology; D008969:Molecular Sequence Data; D010957:Plasmids; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D016019:Survival Analysis
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis