title: Cryopreservation of apices of in vitro plantlets of sugarcane (Saccharum sp. hybrids) using encapsulation/dehydration.
abstract: A cryopreservation process using encapsulation/dehydration was set up for apices sampled on in vitro plantlets of sugarcane. After dissection, apices were cultured for one day on standard medium and then encapsulated in medium with 3% alginate. Optimal conditions comprised preculture for 2 days in liquid medium with 250 g.l(-1) sucrose, desiccation for 6 hours under the laminar flow or for 10-11 hours with silicagel followed by rapid freezing and slow thawing. Survival after freezing in liquid nitrogen ranged between 38 and 91% for the 5 varieties experimented. Cryopreservation did not modify the electrophoretic profiles for aminoleucine peptidases and amylases with plants of the variety Co 6415.
authors.affiliation: CIRAD-CA, BP 5035, 34032, 01, Montpellier cedex, France.
authors.forename: F
authors.initials: F
authors.lastname: Paulet
authors.forename: F
authors.initials: F
authors.lastname: Engelmann
authors.forename: J C
authors.initials: JC
authors.lastname: Glaszmann