title: Micronucleus assay assessment of possible genotoxic effects in patients treated with titanium alloy endosseous implants or miniplates.
abstract: The use of titanium and its alloys (Ti-6Al-4V) for oral surgery has increased dramatically in recent years. Ti is a stable biocompatible metal suitable for oral applications and it has been used for endosseous subperiosteal implants and miniplate fixation for more than 25 years. Dental implants are typically made of Ti or Ti alloys. The alloys are potentially toxic due to release of vanadium and aluminum. We tested the possible genotoxicity of Ti alloy endosseous implants and miniplates on the oral mucosal tissues of two groups of patients: 17 patients receiving Ti miniplate and screw fixation, and 37 endosseous dental implant placement patients. Preoperative and postoperative mucogingival cell samples were collected. Genotoxicity was assessed by the micronucleus assay (MN). There were slight but not statistically significant increases in the frequencies of MN (p=0.087 and p=0.047) post-operation in both groups. In summary, neither of the applications showed genotoxicity in the oral epithelial cells of patients.
authors.affiliation: Gazi University, Faculty of Pharmacy, Toxicology Department, 06330 Ankara, Turkey. Electronic address: bensuka@gmail.com.
authors.forename: Bensu
authors.initials: B
authors.lastname: Karahalil
authors.affiliation: Gazi University, Faculty of Pharmacy, Toxicology Department, 06330 Ankara, Turkey.
authors.forename: Ela
authors.initials: E
authors.lastname: Kadioglu
authors.affiliation: Ankara University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, 06500 Ankara, Turkey.
authors.forename: Ayşegül M
authors.initials: AM
authors.lastname: Tuzuner-Oncul
authors.affiliation: Medicana Dental Hospital, Cifte havuzlar, Istanbul, Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Cimen
authors.affiliation: Gazi University, Faculty of Pharmacy, Toxicology Department, 06330 Ankara, Turkey.
authors.forename: Esra
authors.initials: E
authors.lastname: Emerce
authors.affiliation: Ankara University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, 06500 Ankara, Turkey.
authors.forename: Reha S
authors.initials: RS
authors.lastname: Kisnisci
mesh_terms: D000497:Alloys; D001672:Biocompatible Materials / Q000009:adverse effects*; D002478:Cells, Cultured; D004249:DNA Damage; D003758:Dental Implantation, Endosseous; D015921:Dental Implants / Q000009:adverse effects*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005881:Gingiva / Q000166:cytology; D006801:Humans; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D015162:Micronucleus Tests; D009061:Mouth Mucosa / Q000166:cytology; D011184:Postoperative Period; D057234:Preoperative Period; D014025:Titanium / Q000009:adverse effects*
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D015921:Dental Implants; C031462:titanium alloy (TiAl6V4); D014025:Titanium
keywords: DNA damage; Genotoxicity; Implant; Micronucleus; Mucogingival cells; Titanium alloy