title: eEF1A mediates the nuclear export of SNAG-containing proteins via the Exportin5-aminoacyl-tRNA complex.
abstract: Exportin5 mediates the nuclear export of double-stranded RNAs, including pre-microRNAs, adenoviral RNAs, and tRNAs. When tRNAs are aminoacylated, the Exportin5-aminoacyl (aa)-tRNA complex recruits and coexports the translation elongation factor eEF1A. Here, we show that eEF1A binds to Snail transcription factors when bound to their main target, the E-cadherin promoter, facilitating their export to the cytoplasm in association with the aa-tRNA-Exportin5 complex. Snail binds to eEF1A through the SNAG domain, a protein nuclear export signal present in several transcription factor families, and this binding is regulated by phosphorylation. Thus, we describe a nuclear role for eEF1A and provide a mechanism for protein nuclear export that attenuates the activity of SNAG-containing transcription factors.
authors.affiliation: Instituto de Neurociencias, CSIC-UMH, Avda. Ramón y Cajal s/n, 03550 San Juan de Alicante, Spain. Electronic address: j.mingot@umh.es.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Mingot
authors.forename: Sonia
authors.initials: S
authors.lastname: Vega
authors.forename: Amparo
authors.initials: A
authors.lastname: Cano
authors.forename: Francisco
authors.initials: F
authors.lastname: Portillo
authors.forename: M Angela
authors.initials: MA
authors.lastname: Nieto
mesh_terms: D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D028884:Karyopherins / Q000235:genetics / Q000378:metabolism*; D061986:MCF-7 Cells; D020648:Peptide Elongation Factor 1 / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection; D000097606:Exportin 1 Protein
chemical_list: D015820:Cadherins; D004268:DNA-Binding Proteins; D028884:Karyopherins; D020648:Peptide Elongation Factor 1; D012346:RNA, Transfer, Amino Acyl; D018160:Receptors, Cytoplasmic and Nuclear; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D000097606:Exportin 1 Protein; C446511:XPO5 protein, human