Large-scale deletions of rice plastid DNA in anther culture.
Plastid DNA (ptDNA) in albino rice plants regenerated from pollen by anther culture was investigated by Southern blotting. Of the 20 albino plants investigated, 7 contained ptDNA that had suffered large-scale deletion. The size and location of the deletions differed among the plants. In all cases about 30 kbp of the region containing the PstI-2 fragment (15.7 kbp) had been retained. The deleted ptDNA molecules were retained in calluses derived from the roots of each albino plant.
Hokkaido Central Agricultural Experiment Station, Naganuma, 069, Hokkaido, Japan.
T
T
Harada
T
T
Sato
D
D
Asaka
I
I
Matsukawa