Essential functions for ID proteins at multiple checkpoints in invariant NKT cell development.
Invariant NKT (iNKT) cells display characteristics of both adaptive and innate lymphoid cells (ILCs). Like other ILCs, iNKT cells constitutively express ID proteins, which antagonize the E protein transcription factors that are essential for adaptive lymphocyte development. However, unlike ILCs, ID2 is not essential for thymic iNKT cell development. In this study, we demonstrated that ID2 and ID3 redundantly promoted iNKT cell lineage specification involving the induction of the signature transcription factor PLZF and that ID3 was critical for development of TBET-dependent NKT1 cells. In contrast, both ID2 and ID3 limited iNKT cell numbers by enforcing the postselection checkpoint in conventional thymocytes. Therefore, iNKT cells show both adaptive and innate-like requirements for ID proteins at distinct checkpoints during iNKT cell development.
Department of Pathology, University of Chicago, Chicago, IL 60637;
Mihalis
M
Verykokakis
Veena
V
Krishnamoorthy
Antonio
A
Iavarone
Anna
A
Lasorella
Mikael
M
Sigvardsson
Barbara L
BL
Kee
D019264:Adoptive Transfer; D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis / Q000235:genetics; D002478:Cells, Cultured; D060966:Clonal Selection, Antigen-Mediated; D015332:Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor; D007133:Immunoglobulin Joining Region / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D051797:Inhibitor of Differentiation Protein 2 / Q000235:genetics / Q000502:physiology*; D051796:Inhibitor of Differentiation Proteins / Q000172:deficiency / Q000502:physiology*; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D051741:Kruppel-Like Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D018655:Lymphocyte Count; D038041:Lymphopoiesis / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D055611:Natural Killer T-Cells / Q000166:cytology* / Q000378:metabolism; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D011828:Radiation Chimera; D020825:T-Box Domain Proteins / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000254:growth & development; D000098815:T-bet Transcription Factor
D000945:Antigens, Differentiation, T-Lymphocyte; C497452:Idb2 protein, mouse; D007133:Immunoglobulin Joining Region; D007135:Immunoglobulin Variable Region; D051797:Inhibitor of Differentiation Protein 2; D051796:Inhibitor of Differentiation Proteins; D051741:Kruppel-Like Transcription Factors; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; C513267:Zbtb16 protein, mouse; C067679:Idb3 protein, mouse; D015847:Interleukin-4; D007371:Interferon-gamma