Improved embryoid induction and green shoot regeneration from wheat anthers cultured in medium with maltose.
Anthers from spring wheat (Triticum aestivum L.) genotypes, including six F1 hybrids, were cultured in a modified liquid N6 medium containing either sucrose or maltose. In every case, use of maltose resulted in greater microspore callus induction and green shoot regeneration than culture in sucrose-containing medium. Induction in maltose medium also allowed green shoots to be recovered from crosses that showed only a poor response in other media and from two genotypes that did not respond to modified N6 medium with sucrose. Replacement of sucrose with maltose generally resulted in microspores having a more embryogenic mode of development in which distinct embryoids often formed. The most responsive genotype produced over 200 green shoots/100 anthers when cultured in medium with maltose.
Agricultural Research and Development, Saskatchewan Wheat Pool, 207-112 Research Drive, P.O. Box 1461, S7K 3P7, Saskatoon, Saskatchewan, Canada.
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Orshinsky
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McGregor
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Johnson
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Hucl
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Kartha