The effect of porcine ADM to improve the burn wound healing.
To study the effect of porcine acellular dermal matrix (ADM) on the burn wound healing. Seventy healthy Wistar rats were inflicted with 2 cm second degree burn and divided into 2 groups; one group was treated with porcine ADM and the other with Povidone Iodine Cream. Biopsies were taken on day 1, 3, 5, 7, 10, 14, 21 for histopathological and biochemical analysis to test PCNA, K19, Integrin-β1, PDGF, EGF and FGF. The results revealed relatively better and faster regeneration after treatment of porcine ADM, along with greatly increased synthesis in collagen in the experimental group. PCNA, K19, Integrin-β1 had an increase and then tapered down, and were stronger in the experimental group than in the contrast group during 21 days after burns. PDGF, EGF and FGF levels increased on day 3, peaked on day 5 and then started to decrease, while significantly enhanced expression of relevant growth factors were observed in the experimental group. Porcine ADM stimulate collagen synthesis, stem cells proliferation and differentiation, and the expression of relevant growth factors and ultimately improve the burn wound healing.
Department of Burn Surgery, Fo Shan Hospital of Sun Yat-Sen University Foshan, Guangdong, China.
Xiaodong
X
Chen
Yan
Y
Shi
Bin
B
Shu
Xiaoxia
X
Xie
Ronghua
R
Yang
Lijun
L
Zhang
Shubin
S
Ruan
Yan
Y
Lin
Zepeng
Z
Lin
Rui
R
Shen
Fenggang
F
Zhang
Xiangsheng
X
Feng
Julin
J
Xie
D062088:Acellular Dermis; D000279:Administration, Cutaneous; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002056:Burns / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D002454:Cell Differentiation; D049109:Cell Proliferation; D003094:Collagen / Q000096:biosynthesis; D004195:Disease Models, Animal; D064593:Heterografts; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D011206:Povidone-Iodine / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D063465:Skin Cream / Q000008:administration & dosage; D016038:Skin Transplantation / Q000295:instrumentation*; D013234:Stem Cells / Q000378:metabolism / Q000473:pathology; D013552:Swine; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects
D015415:Biomarkers; D036341:Intercellular Signaling Peptides and Proteins; D011206:Povidone-Iodine; D003094:Collagen
Integrin-β1; K19; PCNA; PDGF; Porcine ADM; deep partial thickness burn wounds