Biogenic nano-scale silver particles by Tephrosia purpurea leaf extract and their inborn antimicrobial activity.
In this paper we report the green synthesis of silver nanoparticles (Ag NPs) using Tephrosia purpurea leaf extract. The biomolecules present in the leaf extract are responsible for the formation of Ag NPs and they found to play dual role of both reducing as well as capping agents. The high crystallinity of Ag NPs is evident from bright circular spot array of SAED pattern and diffraction peaks in XRD profile. The synthesized Ag NPs are found to be nearly spherical ones with size approximately ∼20 nm. FTIR spectrum evidences the presence of different functional groups of biomolecules participated in encapsulating Ag NPs and the possible mechanism of Ag NPs formation was also suggested. Appearance of yellow color and surface plasmon resonance (SPR) peak at 425 nm confirms the Ag NPs formation. PL spectra showed decrement in luminescence intensity at higher excitation wavelengths. Antimicrobial activity of Ag NPs showed better inhibitory activity towards Pseudomonas spp. and Penicillium spp. compared to other test pathogens using standard Kirby-Bauer disc diffusion assay.
Department of Physics, Sri Venkateswara University, Tirupati 517502, India.
B
B
Ajitha
Department of Physics, Sri Venkateswara University, Tirupati 517502, India.
Y Ashok Kumar
YA
Reddy
Department of Physics, Sri Venkateswara University, Tirupati 517502, India. Electronic address: psreddy4@gmail.com.
P Sreedhara
PS
Reddy
D000890:Anti-Infective Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D003460:Crystallization; D005658:Fungi / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D010316:Particle Size; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D012834:Silver / Q000494:pharmacology*; D013052:Spectrometry, X-Ray Emission; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D031304:Tephrosia / Q000737:chemistry*; D014961:X-Ray Diffraction
D000890:Anti-Infective Agents; D010936:Plant Extracts; D012834:Silver
Antimicrobial activity; Green route; Leaf extract; Silver nanoparticles; Surface plasmon resonance; Tephrosia purpurea