Chromogenic sensing of biological thiols using squarylium dye.
A new highly selective probe for biothiols, squarylium dye (SQ), was designed and synthesized. The probe displayed a color change from blue to colorless upon reaction with biothilos such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH). The competition experiments revealed that no obvious interference was observed by performing the titration with the mixtures of Cys and other amino acids. The results indicated that SQ was highly selective for Cys detection. Moreover, SQ could also serve as a "naked-eye" probe for Cys with a minimum detectable concentration of approximately 4 μM.
Department of Advanced Organic Materials Science and Engineering, Kyungpook National University, Daegu 702 701, Republic of Korea.
In-Sub
IS
Shin
Department of Advanced Organic Materials Science and Engineering, Kyungpook National University, Daegu 702 701, Republic of Korea.
Seon-Yeong
SY
Gwon
Department of Advanced Organic Materials Science and Engineering, Kyungpook National University, Daegu 702 701, Republic of Korea; School of Chemistry Science & Technology, Zhanjiang Normal University, Zhanjiang 524048, PR China. Electronic address: shokim@knu.ac.kr.
Sung-Hoon
SH
Kim
D004396:Coloring Agents / Q000138:chemical synthesis / Q000737:chemistry*; D003545:Cysteine / Q000032:analysis*; D005978:Glutathione / Q000032:analysis*; D006710:Homocysteine / Q000032:analysis*; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods; D013438:Sulfhydryl Compounds / Q000032:analysis*
D004396:Coloring Agents; D013438:Sulfhydryl Compounds; D006710:Homocysteine; D005978:Glutathione; D003545:Cysteine
Biothiols; Chemosensor; Cysteine; Glutathione; Homocysteine; Squarylium dye