High-speed electro-fusion and electro-transfection of plant protoplasts by a continuous flow electro-manipulator.
A continuous flow electro-manipulator available both for mass production of fused and of transfected plant protoplasts was devised using a flow chamber with gold-coated glass panel electrodes. Up to 100 ml of protoplasts suspension were treated within 20 min at the rate of approximately 1×10(6) protoplasts / min. The yield of diheterokaryons between tobacco mesophyll and carrot root protoplasts reached approximately 10 % of total protoplasts by flow electro-fusion. More than 95 % of tobacco and cowpea mesophyll protoplasts became infected with tobacco mosaic virus RNA by flow electro-transfection.
National Institute of Agrobiological Resources, 305, Tsukuba Science City, Ibaraki, Japan.
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Hibi
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Kano
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Sugiura
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Kazami
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Kimura