Hypothermic machine perfusion versus cold storage in the rescuing of livers from non-heart-beating donor rats.
The aim of this work was to compare the efficiency of cold storage (CS) and hypothermic machine perfusion (HMP) methods of preserving grafts excised from non-heart-beating donors that had suffered 45 minutes of warm ischemia. We developed a new solution for HMP to use in liver transplantation, based on BES, gluconate, and polyethylene glycol (BGP-HMP solution). After 24 h of HMP or CS, livers were reperfused at 37°C with Krebs-Henseleit solution with added dextran. For both procedures, portal pressure and flow were measured and the intrahepatic resistance (IR) was calculated. The pH oscillations and enzyme activities (LDH, AST, and ALT) were evaluated for the perfusion buffer during normothermic reperfusion. O2 consumption of the liver, glycogen production, and bile flow were also measured during the normothermic reperfusion period. Portal flow and IR showed statistical differences (P < 0.05) between the two groups (n = 5). HMP with BGP-HMP solution resulted in higher values of portal flow and lower IR than CS with HTK solution. Enzyme release after 90 min of reperfusion did not show statistical differences between groups. With regard to bile flow and O2 consumption, livers preserved by both processes were able to produce bile, but livers preserved with HMP were able to take up more O2 than livers preserved by CS.
Binational Center (Argentina-Italy) for Research in Clinical and Applied Cryobiology (CAIC), National University of Rosario, Rosario, Argentina.
Matías E
ME
Carnevale
Cecilia L
CL
Balaban
Edgardo E
EE
Guibert
Hebe
H
Bottai
Joaquin V
JV
Rodriguez
D000818:Animals; D001646:Bile / Q000378:metabolism; D003080:Cold Temperature; D004867:Equipment Design; D008099:Liver / Q000201:enzymology / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D009926:Organ Preservation / Q000295:instrumentation*; D019188:Organ Preservation Solutions / Q000737:chemistry / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010477:Perfusion / Q000295:instrumentation*; D051381:Rats; D017208:Rats, Wistar
D019188:Organ Preservation Solutions; D010100:Oxygen
Cold storage; Hypothermic machine perfusion; Liver; Non-heart-beating donors