Isolation of a selenite-reducing and cadmium-resistant bacterium Pseudomonas sp. strain RB for microbial synthesis of CdSe nanoparticles.
Bacteria capable of synthesizing CdSe from selenite and cadmium ion were enriched from a soil sample. After repeated transfer of the soil-derived bacterial cultures to a new medium containing selenite and cadmium ion 42 times (during 360 days), an enrichment culture that can simultaneously remove selenite and cadmium ion (1 mM each) from the liquid phase was obtained. The culture's color became reddish-brown, indicating CdSe nanoparticle production, as confirmed by energy-dispersive x-ray spectra (EDS). As a result of isolation operations, the bacterium that was the most responsible for synthesizing CdSe, named Pseudomonas sp. RB, was obtained. Transmission electron microscopy and EDS revealed that this strain accumulated nanoparticles (10-20 nm) consisting of selenium and cadmium inside and on the cells when cultivated in the same medium for the enrichment culture. This report is the first describing isolation of a selenite-reducing and cadmium-resistant bacterium. It is useful for CdSe nanoparticle synthesis in the simple one-vessel operation.
Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan.
Hiroyuki
H
Ayano
Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan.
Masaki
M
Miyake
Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan.
Kanako
K
Terasawa
Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan.
Masashi
M
Kuroda
Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan.
Satoshi
S
Soda
Department of Environmental Sciences, Faculty of Life and Environmental Science, Prefectural University of Hiroshima, 562 Nanatsuka-cho, Shobara City, Hiroshima 727-0023, Japan.
Toshifumi
T
Sakaguchi
Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan. Electronic address: ike@see.eng.osaka-u.ac.jp.
Michihiko
M
Ike
D002104:Cadmium / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D019187:Cadmium Compounds / Q000737:chemistry / Q000378:metabolism*; D024881:Drug Resistance, Bacterial / Q000187:drug effects; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism*; D020887:Selenious Acid / Q000032:analysis / Q000378:metabolism*; D012643:Selenium / Q000032:analysis / Q000378:metabolism; D018036:Selenium Compounds / Q000737:chemistry / Q000378:metabolism*; D012988:Soil Microbiology
D019187:Cadmium Compounds; D018036:Selenium Compounds; D002104:Cadmium; C058667:cadmium selenide; D020887:Selenious Acid; D012643:Selenium
Cadmium selenide; Elemental selenium; Enrichment culture; Pseudomonas sp. strain RB; Selenite