SedNMR: a web tool for optimizing sedimentation of macromolecular solutes for SSNMR.
We have proposed solid state NMR (SSNMR) of sedimented solutes as a novel approach to sample preparation for biomolecular SSNMR without crystallization or other sample manipulations. The biomolecules are confined by high gravity--obtained by centrifugal forces either directly in a SSNMR rotor or in a ultracentrifugal device--into a hydrated non-crystalline solid suitable for SSNMR investigations. When gravity is removed, the sample reverts to solution and can be treated as any solution NMR sample. We here describe a simple web tool to calculate the relevant parameters for the success of the experiment.
Center for Magnetic Resonance (CERM), University of Florence, Via L. Sacconi 6, 50019, Sesto Fiorentino, FI, Italy.
Lucio
L
Ferella
Claudio
C
Luchinat
Enrico
E
Ravera
Antonio
A
Rosato
D005591:Chemical Fractionation; D020407:Internet; D046911:Macromolecular Substances / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011506:Proteins / Q000737:chemistry*; D012984:Software; D014461:Ultracentrifugation
D046911:Macromolecular Substances; D011506:Proteins