Label-free and ultrasensitive fluorescence detection of cocaine based on a strategy that utilizes DNA-templated silver nanoclusters and the nicking endonuclease-assisted signal amplification method.
A general and reliable strategy for the detection of cocaine was proposed utilizing DNA-templated silver nanoclusters as signal indicators and the nicking endonuclease-assisted signal amplification method. This strategy can detect cocaine specifically with a detection limit as low as 2 nM by using a small volume of 5 μL.
Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu 214063, China. zhangkai@jsinm.org xieminhao@jsinm.org.
Kai
K
Zhang
Ke
K
Wang
Xue
X
Zhu
Jue
J
Zhang
Lan
L
Xu
Biao
B
Huang
Minhao
M
Xie
D000779:Anesthetics, Local / Q000032:analysis / Q000097:blood*; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D003042:Cocaine / Q000032:analysis / Q000097:blood*; D004720:Endonucleases / Q000378:metabolism; D006801:Humans; D057230:Limit of Detection; D008969:Molecular Sequence Data; D049329:Nanostructures / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D000779:Anesthetics, Local; D052157:Aptamers, Nucleotide; D012834:Silver; D004720:Endonucleases; D003042:Cocaine