Nucleotide pools in suspension-cultured cells of Datura innoxia : II. Correlation with nutrient uptake and macromolecular synthesis.
A general picture of the metabolic events which govern to growth behaviour of a batch culture of suspended dedifferentiated cells of Datura innoxia is obtained by following both the uptake and accumulation of the medium phosphate and sucrose by the cells, and the synthesis of RNA, protein and starch. The results are compared with the changes in the nucleotide pools described in the preceding paper. The sequence of formation and the regulatory dependencies of cellular pools of phosphate, sucrose, nucleotides and RNA in the production of proteins, starch and cell mass, and in the control of proliferation and cell growth are discussed. Furthermore, the importance of the maintenance pools for metabolic survival during starvation is emphasized.
Gesellschaft für Biotechnologische Forschung, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany.
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Wylegalla
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Meyer
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Wagner