The cold signaling attenuator HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE1 activates FLOWERING LOCUS C transcription via chromatin remodeling under short-term cold stress in Arabidopsis.
Exposure to short-term cold stress delays flowering by activating the floral repressor FLOWERING LOCUS C (FLC) in Arabidopsis thaliana. The cold signaling attenuator HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE1 (HOS1) negatively regulates cold responses. Notably, HOS1-deficient mutants exhibit early flowering, and FLC expression is suppressed in the mutants. However, it remains unknown how HOS1 regulates FLC expression. Here, we show that HOS1 induces FLC expression by antagonizing the actions of FVE and its interacting partner histone deacetylase 6 (HDA6) under short-term cold stress. HOS1 binds to FLC chromatin in an FVE-dependent manner, and FVE is essential for the HOS1-mediated activation of FLC transcription. HOS1 also interacts with HDA6 and inhibits the binding of HDA6 to FLC chromatin. Intermittent cold treatments induce FLC expression by activating HOS1, which attenuates the activity of HDA6 in silencing FLC chromatin, and the effects of intermittent cold are diminished in hos1 and fve mutants. These observations indicate that HOS1 acts as a chromatin remodeling factor for FLC regulation under short-term cold stress.
Department of Chemistry, Seoul National University, Seoul 151-742, Korea.
Jae-Hoon
JH
Jung
Ju-Hyung
JH
Park
Sangmin
S
Lee
Taiko Kim
TK
To
Jong-Myong
JM
Kim
Motoaki
M
Seki
Chung-Mo
CM
Park
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000235:genetics; D002843:Chromatin / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly / Q000235:genetics*; D003080:Cold Temperature; D035264:Flowers / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D013312:Stress, Physiological; D014157:Transcription Factors; D014158:Transcription, Genetic
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D002843:Chromatin; C418902:At5g10140 protein, Arabidopsis; C425768:HOS1 protein, Arabidopsis; D047908:Intracellular Signaling Peptides and Proteins; D026161:MADS Domain Proteins; C116635:MSI4 protein, Arabidopsis; D009687:Nuclear Proteins; D014157:Transcription Factors; C432452:AT5G63110 protein, Arabidopsis; D006655:Histone Deacetylases