Effect of exposure to diazinon on adult rat's brain.
Diazinon (DZN), a commonly used agricultural organophosphate insecticide, is one of the major concerns for human health. This study was planned to investigate neurotoxic effects of subacute exposure to DZN in adult male Wistar rats. Animals received corn oil as control and 15 and 30 mg/kg DZN orally by gastric gavage for 4 weeks. The cerebrum malondialdehyde and glutathione (GSH) contents were assessed as biomarkers of lipid peroxidation and nonenzyme antioxidants, respectively. Moreover, activated forms of caspase 3, -9, and Bax/Bcl-2 ratios were evaluated as key apoptotic proteins. Results of this study suggested that chronic administration of DZN did not change lipid peroxidation and GSH levels significantly in comparison with control. Also, the active forms of caspase 3 and caspase 9 were not significantly altered in DZN-treated rat groups. Moreover, no significant changes were observed in Bax and Bcl-2 ratios. This study indicated that generation of reactive oxygen species was probably modulated by intracellular antioxidant system. In conclusion, subacute oral administration of DZN did not alter lipid peroxidation. Moreover, apoptosis induction was not observed in rat brain.
Department of Pharmacodynamy and Toxicology, Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Islamic Republic of Iran.
Marzieh
M
Rashedinia
Department of Pharmacodynamy and Toxicology, Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Islamic Republic of Iran.
Hossein
H
Hosseinzadeh
Department of Pharmacodynamy and Toxicology, Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Islamic Republic of Iran.
Mohsen
M
Imenshahidi
Department of Pharmacodynamy and Toxicology, Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Islamic Republic of Iran.
Parisa
P
Lari
Department of Pharmacodynamy and Toxicology, Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Islamic Republic of Iran.
Bibi Marjan
BM
Razavi
Department of Medicinal Chemistry and Department of Biotechnology, Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Islamic Republic of Iran abnouskh@mums.ac.ir.
Khalil
K
Abnous
D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001921:Brain / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D001923:Brain Chemistry / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D003976:Diazinon / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D051017:Apoptosis Regulatory Proteins; D007306:Insecticides; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D020169:Caspases; D003976:Diazinon
Bax; Bcl-2; Diazinon; caspase 3; caspase 9; neurotoxicity; subacute toxicity