Re-annotation of the genome sequence of Helicobacter pylori 26695.
Helicobacter pylori is a pathogenic bacterium that colonizes the human epithelia, causing duodenal and gastric ulcers, and gastric cancer. The genome of H. pylori 26695 has been previously sequenced and annotated. In addition, two genome-scale metabolic models have been developed. In order to maintain accurate and relevant information on coding sequences (CDS) and to retrieve new information, the assignment of new functions to Helicobacter pylori 26695s genes was performed in this work. The use of software tools, on-line databases and an annotation pipeline for inspecting each gene allowed the attribution of validated EC numbers and TC numbers to metabolic genes encoding enzymes and transport proteins, respectively. 1212 genes encoding proteins were identified in this annotation, being 712 metabolic genes and 500 non-metabolic, while 191 new functions were assignment to the CDS of this bacterium. This information provides relevant biological information for the scientific community dealing with this organism and can be used as the basis for a new metabolic model reconstruction.
IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Portugal.
Tiago
T
Resende
Daniela M
DM
Correia
Miguel
M
Rocha
Isabel
I
Rocha
D016680:Genome, Bacterial; D016480:Helicobacter pylori / Q000235:genetics*; D012984:Software