Correlative light microscopy for high-content screening.
High-throughput microscopy is an effective tool for rapidly collecting data on a large scale. However, high throughput comes at the cost of low spatial resolution. Here we introduce correlative light microscopy by combining fast automated widefield imaging, confocal microscopy and super-resolution microscopy. We demonstrate the potential of this approach for scalable experiments. The workflow consists of a robust approach for selecting cells of interest on a wide-field screening microscope at low resolution and subsequently re-localizing those cells with micrometer precision for confocal and super-resolution imaging. As a case study, we visualized and quantified cis- and trans-Golgi markers at increasing resolution.
BioQuant Centre, Heidelberg University, Heidelberg, Germany.
Benjamin
B
Flottmann
Manuel
M
Gunkel
Tautvydas
T
Lisauskas
Mike
M
Heilemann
Vytaute
V
Starkuviene
Jürgen
J
Reymann
Holger
H
Erfle
D000818:Animals; D002460:Cell Line; D006056:Golgi Apparatus / Q000648:ultrastructure*; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007668:Kidney / Q000166:cytology; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D051381:Rats