Night milking adds value to cow's milk.
BACKGROUND
Melatonin is synthesized in greater concentration at night, and it plays an important role in sleep regulation. This study aimed to evaluate the melatonin concentration in milk collected by milking during the night and evaluates its effect, with or without tryptophan supplementation, in the sleep quality of adult Wistar rats.


RESULTS
A difference (P < 0.05) was observed between the milking times, where the milk obtained at 02:00 presented a higher melatonin concentration (39.43 pg mL(-1) ) than that acquired at 15:00 (4.03 pg mL(-1) ). A biological assay was also performed on 32 male adult Wistar rats distributed among four groups (n = 8): those receiving an AIN-93M diet (control group) and three test groups [diets containing milk from milking at 02:00 (M2h ), milking at 15:00 (M15h ), and milking at 02:00 plus tryptophan supplementation (M2h T)] for 28 days. It was observed that the control group did not differ (P > 0.05) from the M15h group in terms of the levels of blood melatonin and urinary sulfatoxymelatonin, but differed from groups M2h and M2h T, whereas group M2h T presented higher blood melatonin and urinary sulfatoxymelatonin concentrations.


CONCLUSIONS
Combining the techniques of night milking with tryptophan supplementation resulted in production of milk that improves sleep quality in rats.
Department of Chemistry, State University of Southwest Bahia, Brazil.
Maria P
MP
Milagres
Valeria P R
VP
Minim
Luis A
LA
Minim
Andrea A
AA
Simiqueli
Liliane E S
LE
Moraes
Hércia S D
HS
Martino
D000818:Animals; D002417:Cattle / Q000502:physiology*; D002940:Circadian Rhythm / Q000502:physiology*; D003612:Dairying / Q000379:methods*; D004032:Diet; D019587:Dietary Supplements; D005260:Female; D007774:Lactation; D008297:Male; D008550:Melatonin / Q000032:analysis* / Q000097:blood; D008892:Milk / Q000737:chemistry* / Q000382:microbiology; D051381:Rats; D017208:Rats, Wistar; D012890:Sleep / Q000187:drug effects; D013997:Time Factors; D014364:Tryptophan / Q000008:administration & dosage
D014364:Tryptophan; D008550:Melatonin
melatonin; milk; sleep; tryptophan