Use of chitosan and β-tricalcium phosphate, alone and in combination, for bone healing in rabbits.
The aim of this research was to evaluate the process of bone regeneration in rabbits, using chitosan and beta-tricalcium phosphate (β-TCP) independently and in combination. A total of 12 New Zealand rabbits of both sexes, with average weight of 3.0 ± 0.57 kg were used. Animals were randomly divided into two experimental time points, with six animals euthanized 45 days after surgery and six euthanized 90 days after surgery. We performed two osteotomies in each tibia. The left tibia was used for the chitosan (QUI) and control groups, and the right tibia was used for the β-TCP alone and in combination with chitosan (QUI+TCP) groups. Tomographic evaluation showed no statistically significant difference among groups; however radiopacity was higher in the treated groups. Comparative descriptive histological evaluation found that treatment groups stimulated a more pronounced tissue repair reaction and accelerated bone repair. Morphometric analysis showed that treatment groups presented statistically higher bone formation compared with the control group.
Unidade acadêmica de Medicina Veterinária (UAMV), Centro de Saúde e Tecnologia Rural (CSTR), Universidade Federal de Campina Grande (UFCG), Av. Universitária s/n, Bairro Santa Cecília, Patos, PB, 58700-970, Brazil, adilio_vet@yahoo.com.br.
A S
AS
Azevedo
M J C
MJ
Sá
M V L
MV
Fook
P I Nóbrega
PI
Neto
O B
OB
Sousa
S S
SS
Azevedo
M W
MW
Teixeira
F S
FS
Costa
A L
AL
Araújo
D000818:Animals; D001842:Bone and Bones / Q000503:physiopathology*; D002130:Calcium Phosphates / Q000494:pharmacology*; D048271:Chitosan / Q000494:pharmacology*; D005260:Female; D017102:Fracture Healing; D008297:Male; D011817:Rabbits; D014057:Tomography, X-Ray Computed
D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D048271:Chitosan