Genetic analysis of 15 STR loci from a Xibe population in Liaoning, Northeast of China.
BACKGROUND
In view of their high level of variability, autosomal short tandem repeats (STRs) are very useful as markers in the disciplines of forensic and population genetics studies.


OBJECTIVE
To investigate the diversity distributions of allelic frequencies of 15 loci from a sample from the Chinese Xibe ethnic group in Liaoning.


METHODS
Fifteen STR loci for 150 unrelated Xibe individuals from Liaoning, China were amplified simultaneously in a fluorescence-based reaction using a 2720 Thermal cycler (ABI). Separation and detection of the amplified product were conducted with the Li-COR 4300 DNA Analyzer.


RESULTS
In total, 117 alleles were observed, with the corresponding allele frequencies ranging from 0.001 to 0.507. D18S51 had the highest polymorphism (PIC = 0.840) among all 15 STR loci, whereas TPOX had the lowest (PIC = 0.590). The power of discrimination ranged from 0.801 for TH01 locus to 0.957 for D18S51 locus, whereas the power of exclusion ranged from a minimum 0.316 for TPOX locus to a maximum 0.720 for D21S11 locus. The phylogenetic tree established among worldwide populations showed that the Xibe population is far from other populations.


CONCLUSIONS
Databases for the 15 STR loci will be useful for personal identification and paternity tests in the Xibe population and for the establishment of phylogenetic relationships between populations.
Department of Cell Biology and Genetics, Shenyang Medical College , Shenyang, Liaoning , PR China.
Jian
J
Liu
Shu-yin
SY
Li
Jiao-yang
JY
Yin
Wei
W
Zhang
Bing
B
Gao
Li
L
Guo
Rong
R
Qi
D002681:China / Q000208:ethnology; D005006:Ethnicity / Q000235:genetics*; D005787:Gene Frequency; D006801:Humans; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic