Primary structure and sequence organization of the 16S-23S spacer in the ribosomal operon of soybean (Glycine max L.) chloroplast DNA.
The nucleotide sequence of a spacer region between 16S and 23S rRNA genes from soybean chloroplasts has been determined. The spacer region is over 3000 bp long and contains two tRNA genes, coding for rRNA(Ile) and tRNA(Ala) which contain intervening sequences of 953 and 811 base pairs respectively. There is a strong homology between the two introns suggesting that they have a common origin. These spacer tRNAs are synthesized as part of a kb precursor molecule containing 16S and 23S rRNA sequences.
Department of Biological Sciences, University of Windsor, N9B 3P4, Windsor, Ontario, Canada.
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de Lanversin
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Pillay