CD1d(hi)CD5⁺ B cells differentiate into antibody-secreting cells under the stimulation with calreticulin fragment.
Calreticulin (CRT) is a multifunctional molecule in both intracellular and extracellular environment. We have previously found that a recombinant CRT fragment (rCRT/39-272) could modulate T cell-mediated immunity in mice via activation and expansion of CD1d(hi)CD5⁺ B cells as well as induction of CRT-specific regulatory antibodies. Antibody secreting cells (ASCs) are terminally differentiated B cells responsible for producing antibodies to participate in positive immune response as well as immune regulation. In this study, we demonstrate that rCRT/39-272 differentiates murine CD1d(hi)CD5⁺ B cells into ASCs marked by increased expression of plasma cell-associated transcription factors and production of polyreactive antibodies against DNA and CRT in vitro. Intraperitoneal administration of rCRT/39-272 augmented differentiation of CD1d(hi)CD5⁺ B cells into ASCs in naïve mice or mice with experimental autoimmune encephalomyelitis. Thus, we propose that ASC differentiation and subsequent antibody production of CD1d(hi)CD5⁺ B cells are key steps in CRT-mediated immunoregulation on inflammatory T cell responses.
Institutes of Biology and Medical Sciences, Soochow University, Suzhou, 215123, China.
Tengteng
T
Zhang
Yun
Y
Xia
Lijuan
L
Zhang
Wanrong
W
Bao
Chao
C
Hong
Xiao-Ming
XM
Gao
D000818:Animals; D055610:Antigens, CD1d / Q000378:metabolism*; D001323:Autoantibodies / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000166:cytology* / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D018956:CD5 Antigens / Q000378:metabolism*; D037282:Calreticulin / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology; D006801:Humans; D051379:Mice; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D012995:Solubility
D055610:Antigens, CD1d; D001323:Autoantibodies; D018956:CD5 Antigens; D037282:Calreticulin; D010446:Peptide Fragments