Analgesic and antiinflammatory activities of the ethyl acetate fraction of Bidens pilosa (Asteraceae).
Bidens pilosa is an Asteraceae widely used in traditional medicine for the treatment of various ailments including pain and inflammation. The present work was undertaken to assess the analgesic and antiinflammatory properties of the ethyl acetate fraction of methylene chloride/methanol (1:1) extract of leaves of Bidens pilosa at the gradual doses of 50, 100 and 200 mg/kg in mice and rats, respectively. The analgesic properties of Bidens pilosa were investigated using the acetic acid writhing, hot plate, capsaicin and formalin-induced pain models. This was followed by a study of the antiinflammatory properties using carrageenan, dextran, histamine and serotonin to induce acute inflammation in rat hind paw. The extract provided a significant (p < 0.01) reduction in pain induced by all four models of nociception. It also presented significant (p < 0.05) antiinflammatory activity in all four models of acute inflammation. These results show that the ethyl acetate fraction of methylene chloride/methanol (1:1) of Bidens pilosa has both analgesic and antiinflammatory properties. The qualitative analysis of the fraction by the high-performance liquid chromatography (HPLC) fingerprint revealed the presence of two flavonoids, namely quercetin and iso-okanin, known to have antiinflammatory and antinociceptive properties, which could be responsible for the analgesic and antiinflammatory effects observed.
Laboratory of Animal Physiology, Department of Biological Science, Higher Teachers' Training College, University of Yaoundé I, P.O. Box 47, Yaoundé, Cameroon, aurelien74000618@yahoo.fr.
Aurélien Fotso
AF
Fotso
Frida
F
Longo
Paul Désiré Dzeufiet
PD
Djomeni
Siméon Fogue
SF
Kouam
Michael
M
Spiteller
Alain Bertrand
AB
Dongmo
J P
JP
Savineau
D000085:Acetates / Q000737:chemistry*; D000700:Analgesics / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D031176:Bidens / Q000737:chemistry*; D047188:Chalcones / Q000737:chemistry / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology; D007249:Inflammation / Q000188:drug therapy*; D008297:Male; D008519:Medicine, Traditional; D051379:Mice; D010146:Pain / Q000188:drug therapy; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D011794:Quercetin / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D000085:Acetates; D000700:Analgesics; D000893:Anti-Inflammatory Agents; D047188:Chalcones; D005419:Flavonoids; D010936:Plant Extracts; C550307:okanin; C007650:ethyl acetate; D011794:Quercetin