Silk fibroin and 4-hexylresorcinol incorporation membrane for guided bone regeneration.
The objective of this study was to demonstrate that a silk fibroin (SF) and 4-hexylresorcinol (4-HR) incorporation membrane could be used for a guided bone regeneration technique. Fourier transform infrared measurements were obtained to determine change of physical property of SF membrane by 4-HR incorporation. Two peri-implant defects, 3.0 × 5.0 mm (width × length), were prepared on the lateral side of the implant hole in the tibia of New Zealand white rabbits (n = 8). The peri-implant defect was left unfilled in the control group. Silk fibroin + 4-HR membrane was applied to the peri-implant defect in the experimental group. The 8 animals were killed at 8 weeks after implantation. Subsequently, removal torque test and histomorphometric evaluation were done. Fourier transform infrared spectroscopy showed no specific chemical interaction between 4-HR and SF. In the histomorphometric analysis, the mean bone regeneration was 18.3 ± 1.9 mm(2) in the experimental group and 9.3 ± 0.9 mm(2) in the control group (P = 0.004). In conclusion, the SF and 4-HR incorporation membrane successfully regenerated bone in the rabbit tibia peri-implant bone defect model.
From the *Department of Oral and Maxillofacial Surgery, Gangneung-Wonju National University, Gangneung; †Department of Dentistry, Jeju National University, Jeju; ‡Sericultural and Apicultural Materials Division, National Academy of Agricultural Science, RDA, Suwon; and §Department of Oral and Maxillofacial Surgery, Sacred Heart Hospital, Hallym University, Anyang, Republic of Korea.
Sang-Woon
SW
Lee
Seong-Gon
SG
Kim
Ji-Young
JY
Song
Haeyong
H
Kweon
You-Young
YY
Jo
Kwang-Gill
KG
Lee
Seok-Woo
SW
Kang
Byoung-Eun
BE
Yang
D000818:Animals; D001847:Bone Diseases / Q000473:pathology / Q000628:therapy; D001861:Bone Regeneration / Q000502:physiology*; D003758:Dental Implantation, Endosseous / Q000379:methods; D015921:Dental Implants; D005349:Fibroins / Q000737:chemistry / Q000627:therapeutic use*; D048091:Guided Tissue Regeneration / Q000295:instrumentation*; D006604:Hexylresorcinol / Q000737:chemistry / Q000627:therapeutic use*; D008567:Membranes, Artificial; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D010012:Osteogenesis / Q000502:physiology; D011817:Rabbits; D047011:Silk; D017550:Spectroscopy, Fourier Transform Infrared; D013977:Tibia / Q000473:pathology / Q000601:surgery; D013997:Time Factors; D019415:Torque
D015921:Dental Implants; D008567:Membranes, Artificial; D016328:NF-kappa B; D047011:Silk; D005349:Fibroins; D006604:Hexylresorcinol