Circulating miR-30a, miR-126 and let-7b as biomarker for ischemic stroke in humans.
BACKGROUND
Recently, plasma miRNAs have been reported as biomarkers for various diseases. However, the knowledge on the association of plasma miRNAs with ischemic stroke is still lacking. In this study, we investigated whether plasma concentrations of miR-30a, miR-126 and let-7b may be biomarkers for ischemic stroke in humans.


METHODS
One hundred ninety seven patients with ischemic stroke were recruited and their blood samples were collected at 24 h, 1 week, 4 weeks, 24 weeks and 48 weeks after symptoms onset, and fifty healthy volunteers were selected as control. Levels of miRNA were quantified by quantitative real-time PCR. Relative expression level of miRNA was calculated using 2(-ΔΔct) method. The ability to distinguish the ischemic stroke group from control group was characterized by receiver operating characteristic (ROC) curve, and the area under ROC curve (AUC) was calculated.


RESULTS
Circulating miR-30a and miR-126 levels were markedly down-regulated in all patients with ischemic stroke until 24 weeks. However, circulating let-7b was lower in patients with large-vessel atherosclerosis than healthy volunteers, whereas circulating let-7b had higher level in patients with other kinds of ischemic stroke until 24 weeks. Among all patients, circulating miRNAs levels returned to normal 48 weeks after symptom onset. Receiver operating characteristic (ROC) curve analysis showed that the areas under the curve (AUC) of plasma miR-30a were 0.91, 0.91, 0.92 and 0.93, the miR-126 were 0.92, 0.94, 0.93 and 0.92, and let-7b were 0.93, 0.92, 0.92 and 0.91 at 24 h, 1 w, 4 w and 24 w, respectively.


CONCLUSIONS
These data suggest that miR-30a, miR-126 and let-7b might be useful biomarkers for ischemic stroke in humans.
Guangwen
G
Long
Feng
F
Wang
Huaping
H
Li
Zhongwei
Z
Yin
Chaugai
C
Sandip
Yan
Y
Lou
Yan
Y
Wang
Department of Internal Medicine and the Institute of Hypertension, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China. chenchen@tjh.tjmu.edu.cn.
Chen
C
Chen
Dao Wen
DW
Wang
D000368:Aged; D015415:Biomarkers / Q000097:blood; D002545:Brain Ischemia / Q000097:blood* / Q000175:diagnosis / Q000235:genetics; D003430:Cross-Sectional Studies; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D006801:Humans; D002537:Intracranial Arteriosclerosis / Q000097:blood / Q000175:diagnosis / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D020521:Stroke / Q000097:blood* / Q000175:diagnosis / Q000235:genetics
D015415:Biomarkers; C527111:MIRN126 microRNA, human; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; C487931:mirnlet7 microRNA, human