Upregulation of long noncoding RNA SPRY4-IT1 modulates proliferation, migration, apoptosis, and network formation in trophoblast cells HTR-8SV/neo.
SPRY4-IT1 has been reported to have extremely high expression in normal placenta tissues. It is a Long noncoding RNA (lncRNA), which is associated with cell growth, migration, invasion, and apoptosis in melanoma. A 2.8-fold increase of SPRY4-IT1 expression was validated by Real-time reverse transcription-polymerase chain reaction (qRT-PCR) in severe preeclamptic placenta as compared with that of the normal ones (n=25) in this study. Furthermore, the role of SPRY4-IT1 in proliferation, migration, apoptosis, and network formation ability of trophoblast cells HTR-8/SVneo was assessed. Suppression of SPRY4-IT1 using siRNA treatment and its overexpression using plasmid targeting SPRY4-IT1 were performed in order to explore the biological function of SPRY4-IT1 in the development and progression of trophoblast cells HTR-8/SVneo, in vitro. The results showed that SPRY4-IT1 knockdown enhanced the cell migration and proliferation, and reduced the response of cells to apoptosis. However, exogenous SPRY4-IT1 overexpression significantly decreased the cell migration and proliferation, while increased cell apoptosis. Our study showed for the first time that aberrant expression of lncRNA SPRY4-IT1 might contribute to the abnormal condition of trophoblast cells HTR-8/SVneo. Therefore, we proposed SPRY4-IT1 as a novel lncRNA molecule, which might be associated with the pathogenesis of preeclampsia and might provide a new target for its early diagnosis and treatment.
Department of Obstetrics and Gynecology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, JiangSu Province, China.
Yanfen
Y
Zou
Ziyan
Z
Jiang
Xiang
X
Yu
Ming
M
Sun
Yuanyuan
Y
Zhang
Qing
Q
Zuo
Jing
J
Zhou
Nana
N
Yang
Ping
P
Han
Zhiping
Z
Ge
Wei
W
De
Lizhou
L
Sun
D000328:Adult; D017209:Apoptosis; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D010920:Placenta / Q000166:cytology / Q000473:pathology; D011225:Pre-Eclampsia / Q000378:metabolism / Q000473:pathology; D011247:Pregnancy; D062085:RNA, Long Noncoding / Q000235:genetics*; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism / Q000473:pathology; D015854:Up-Regulation; D055815:Young Adult
D062085:RNA, Long Noncoding