Pretreatment of urine samples with SDS improves direct identification of urinary tract pathogens with matrix-assisted laser desorption ionization-time of flight mass spectrometry.
We pretreated with SDS 71 urine samples with bacterial counts of >10(5) CFU/ml and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) identification scores of <2, in order to minimize failure rates. Identification improved in 46.5% of samples, remained unchanged in 49.3%, and worsened in 4.2%. The improvement was more evident for Gram-negative (54.3%) than for Gram-positive (32%) bacteria.
Unidad de Investigación, Complejo Asistencial Universitario de Salamanca, Salamanca, Spain.
F
F
Sánchez-Juanes
M
M
Siller Ruiz
F
F
Moreno Obregón
M
M
Criado González
S
S
Hernández Egido
M
M
de Frutos Serna
J M
JM
González-Buitrago
J L
JL
Muñoz-Bellido
D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis*; D003902:Detergents / Q000494:pharmacology; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013048:Specimen Handling / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014552:Urinary Tract Infections / Q000175:diagnosis*; D014556:Urine / Q000382:microbiology*
D003902:Detergents; D012967:Sodium Dodecyl Sulfate