Regeneration from long-term cell suspension cultures of tepary bean (Phaseolus acutifolius).
Plant regeneration has been achieved from long-term cell suspension cultures established from leaf derived callus of tepary bean (Phaseolus acutifolius). The proportion of densely cytoplasmic cells in suspension culture increased when cultured in the L-6 medium with 54 μM NAA and 2 μM KN. Filtration of the cells at each of five consecutive subcultures resulted in the isolation of a plant regenerating cell line (TB 686), which is being maintained in L-6 medium with 4.5 μM 2,4-D and 2.3 μM zeatin. Differentiated green cell aggregates were obtained when cells from maintenance medium were transferred to the same medium with 10 μM BA. Embryo-like structures developed from these aggregates on L-6 medium with 2.3 μM zeatin, 0.69 μM GA3 and 1.5 μM NAA. Plantlets regenerated from these structures when they were cultured on L-6 medium with 7.0 μM NAA and 1.0 μM KN. Plant regeneration from the cell line remained relatively constant for 270 days. Regenerated plants were grown to maturity in the greenhouse.
Tissue Culture for Crops Project, Department of Biology, Botany Section, Colorado State University, 80523, Fort Collins, CO, USA.
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Kumar
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Gamborg
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Nabors