Evaluation of the comet assay for assessing the dose-response relationship of DNA damage induced by ionizing radiation.
Dose- and time-response curves were combined to assess the potential of the comet assay in radiation biodosimetry. The neutral comet assay was used to detect DNA double-strand breaks in lymphocytes caused by γ-ray irradiation. A clear dose-response relationship with DNA double-strand breaks using the comet assay was found at different times after irradiation (p < 0.001). A time-response relationship was also found within 72 h after irradiation (p < 0.001). The curves for DNA double-strand breaks and DNA repair in vitro of human lymphocytes presented a nice model, and a smooth, three-dimensional plane model was obtained when the two curves were combined.
Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China. liuqiang@irm-cams.ac.cn.
Yan
Y
Wang
Chang
C
Xu
Li Qing
LQ
Du
Jia
J
Cao
Jian Xiang
JX
Liu
Xu
X
Su
Hui
H
Zhao
Fei-Yue
FY
Fan
Bing
B
Wang
Takanori
T
Katsube
Sai Jun
SJ
Fan
Qiang
Q
Liu
D020552:Comet Assay / Q000379:methods*; D053903:DNA Breaks, Double-Stranded / Q000528:radiation effects*; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects*; D011839:Radiation, Ionizing