Stimulation of corticotropin-releasing factor gene expression by FosB in rat hypothalamic 4B cells.
The Fos- and Jun family proteins are immediate-early gene products, and the Fos/Jun heterodimer, activator protein-1 (AP-1), may be involved in the regulation of corticotropin-releasing factor (CRF) gene expression. FosB is a member of the Fos family proteins that is expressed in the paraventricular nucleus of the hypothalamus upon stress exposure, but it has not been clear whether FosB participates in the regulation of CRF gene expression. This study aimed to explore the effect of the FosB and cJun proteins on CRF gene expression in rat hypothalamic 4B cells. The levels of FosB mRNA and cJun mRNA increased following treatment with forskolin, phorbol-12-myristate-13-acetate (PMA), or A23187 in the hypothalamic cells. Overexpression of FosB or cJun potently increased CRF mRNA levels. Furthermore, downregulation of FosB or cJun suppressed the CRF gene expression induced by forskolin, PMA, or A23187. In addition, the basal CRF mRNA levels were partially reduced by cJun downregulation. These findings suggest that FosB, together with cJun, may mediate CRF gene expression in the hypothalamic cells.
Department of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan; Department of Endocrinology, Metabolism, and Infectious Diseases, Hirosaki University School of Medicine & Hospital, Hirosaki 036-8563, Japan. Electronic address: kkageyama@hkg.odn.ne.jp.
Kazunori
K
Kageyama
Laboratory of Information Biology, Graduate School of Information Sciences, Tohoku University, Sendai 980-8579, Japan.
Keiichi
K
Itoi
Health Care Center, Kochi University, Kochi 780-8520, Japan.
Yasumasa
Y
Iwasaki
Department of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan.
Kanako
K
Niioka
Department of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan.
Yutaka
Y
Watanuki
Department of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan.
Satoshi
S
Yamagata
Department of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan.
Yuki
Y
Nakada
Laboratory of Information Biology, Graduate School of Information Sciences, Tohoku University, Sendai 980-8579, Japan.
Gopal
G
Das
Department of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan.
Toshihiro
T
Suda
Department of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan.
Makoto
M
Daimon
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D003346:Corticotropin-Releasing Hormone / Q000235:genetics* / Q000378:metabolism; D020536:Enzyme Activators / Q000494:pharmacology; D015870:Gene Expression; D007031:Hypothalamus / Q000166:cytology*; D055099:Neuroendocrine Cells / Q000187:drug effects / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000502:physiology*; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D015290:Second Messenger Systems; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015533:Transcriptional Activation
D020536:Enzyme Activators; C483820:Fosb protein, rat; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D005576:Colforsin; D000001:Calcimycin; D003346:Corticotropin-Releasing Hormone; D000262:Adenylyl Cyclases; D013755:Tetradecanoylphorbol Acetate
Corticotropin-releasing factor; FosB; Hypothalamus; Jun; Stress