Sepharose-immobilized triazine dyes as adsorbants for human lymphoblastoid interferon purification.
An extensive selection of immobilized triazine dyes have been examined for their potential as adsorbants for human lymphobalstoid interferon. Procion red HE7B was selected as the most suitable for preparative scale purification. Sepharose-immobilized procion red HE7B is able to bind 10(5) reference units/mL of interferon from cell supernatants and can be eluted with at least 25-fold purification and 90% yield by a KC1 gradient. Further purification was obtained either by reapplying the eluted interferon after dialysis to the dye column or by gel filtration on Ultrogel AcA 34 after lyophilization and dialysis. The latter procedure gave a final activity of about 10(6) U/mg protein and approximately 75% recovery of interferon activity.
Molecular Biology Department, The Wellcome Research Laboratories, Research Triangle Park, 27709, NC.
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