Role of invariant natural killer T cells in lipopolysaccharide-induced pregnancy loss.
We aimed to investigate the role of invariant natural killer T (iNKT) cells in infection-associated pregnancy loss. Wild-type (WT) C57BL/6 mice and iNKT cell-deficient Jα18(-/-) mice were treated with lipopolysaccharide (LPS). Embryo resorption rates (ERRs), decidual costimulatory molecule and activation molecule expression, and cytokine production were determined. WT and Jα18(-/-) mice were adoptively transferred with purified iNKT cells. ERRs, decidual costimulatory molecule and activation molecule expression, and cytokine production were assessed. LPS-treated Jα18(-/-) mice showed markedly reduced ERRs, decreased CD40, CD80, CD86, and CD69 expression, and reduced Th1 cytokine production at the maternal-fetal interface compared with WT mice. ERRs, expression of CD40, CD80, CD86, and CD69, and Th1 cytokine production in LPS-injected Jα18(-/-) mice following iNKT cell adoptive transfer were remarkably upregulated compared with control mice that did not receive adoptively transferred iNKT cells. Our results suggest that iNKT cells play an important role in LPS-induced pregnancy loss.
Department of Obstetrics and Gynecology, Guangzhou Medical University Affiliated Guangzhou First People's Hospital, Guangzhou 510180, China.
Liping
L
Li
Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200001, China.
Lijuan
L
Shi
Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200001, China.
Xinying
X
Yang
Department of Obstetrics and Gynecology, Guangzhou Medical University Affiliated Guangzhou First People's Hospital, Guangzhou 510180, China.
Lihua
L
Ren
Department of Obstetrics and Gynecology, Guangzhou Medical University Affiliated Guangzhou First People's Hospital, Guangzhou 510180, China.
Jing
J
Yang
Bio-X Institutes, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: yilinonline@126.com.
Yi
Y
Lin
D019264:Adoptive Transfer; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D003656:Decidua / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology*; D020964:Embryo Loss / Q000139:chemically induced / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D004622:Embryo, Mammalian; D005260:Female; D015870:Gene Expression / Q000276:immunology; D007274:Injections, Intraperitoneal; D008070:Lipopolysaccharides; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D055611:Natural Killer T-Cells / Q000276:immunology / Q000473:pathology* / Q000637:transplantation; D011247:Pregnancy; D018417:Th1 Cells / Q000276:immunology / Q000473:pathology*; D058408:Th1-Th2 Balance; D015854:Up-Regulation
D015703:Antigens, CD; D016207:Cytokines; D008070:Lipopolysaccharides
Inflammation; Invariant natural killer T cells; Lipopolysaccharide; Pregnancy loss; Toll-like receptor