Energy and lipid metabolism gene expression of D18 embryos in dairy cows is related to dam physiological status.
We analyzed the change in gene expression related to dam physiological status in day (D)18 embryos from growing heifers (GH), early lactating cows (ELC), and late lactating cows (LLC). Dam energy metabolism was characterized by measurement of circulating concentrations of insulin, glucose, IGF-1, nonesterified fatty acids, β-hydroxybutyrate, and urea before embryo flush. The metabolic parameters were related to differential gene expression in the extraembryonic tissues by correlation analysis. Embryo development estimated by measuring the length of the conceptuses and the proportion of expected D18 gastrulating stages was not different between the three groups of females. However, embryo metabolism was greatly affected by dam physiological status when we compared GH with ELC and GH with LLC but to a lesser extent when ELC was compared with LLC. Genes involved in glucose, pyruvate, and acetate utilization were upregulated in GH vs. ELC conceptuses (e.g., SLC2A1, PC, ACSS2, ACSS3). This was also true for the pentose pathway ( PGD, TKT), which is involved in synthesis of ribose precursors of RNA and DNA. The pathways involved in lipid synthesis were also upregulated in GH vs. ELC. Despite similar morphological development, the molecular characteristics of the heifers' embryos were consistently different from those of the cows. Most of these differences were strongly related to metabolic/hormone patterns before insemination and during conceptus free-life. Many biosynthetic pathways appeared to be more active in heifer embryos than in cow embryos, and consequently they seemed to be healthier, and this may be more conducive to continue development.
INRA, UMR 1198 Biologie du Développement et Reproduction, Jouy-en-Josas, France;
D
D
Valour
S A
SA
Degrelle
A A
AA
Ponter
C
C
Giraud-Delville
E
E
Campion
C
C
Guyader-Joly
C
C
Richard
F
F
Constant
P
P
Humblot
C
C
Ponsart
I
I
Hue
B
B
Grimard
D020155:3-Hydroxybutyric Acid / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000502:physiology; D002417:Cattle; D016000:Cluster Analysis; D004622:Embryo, Mammalian / Q000196:embryology / Q000378:metabolism*; D004734:Energy Metabolism / Q000502:physiology*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007328:Insulin / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007774:Lactation / Q000502:physiology; D050356:Lipid Metabolism / Q000502:physiology*; D008297:Male; D008892:Milk / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D055703:Reproductive Physiological Phenomena; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014508:Urea / Q000097:blood
D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D007334:Insulin-Like Growth Factor I; D014508:Urea; D020155:3-Hydroxybutyric Acid
conceptus development; correlations; dairy cow; energy balance; energy metabolism; fertility; gene expression