Direct gene transfer to protoplasts of Arabidopsis thaliana.
We performed a series of direct gene transfer experiments with protoplasts of Arabidopsis thaliana ecotype Zürich. An average of more than 100 transformants were selected per 10(6)6 treated protoplasts. Stable transformation was confirmed by integration of the marker gene into high molecular weight DNA and by its genetic transmission to subsequent offspring generations.
Institute of Plant Sciences, Swiss Federal Institute of Technology (ETH), ETH-Zentrum, CH-8092, Zürich, Switzerland.
H
H
Karesch
R
R
Bilang
O
O
Mittelsten Scheid
I
I
Potrykus