Biostable ssDNA aptamers specific for Hodgkin lymphoma.
As a "chemical antibody", oligonucleotide aptamers can specifically bind to their target molecules. However, clinical potential of aptamers in disease diagnosis is not yet fully explored. Using a tumor cell-based selection protocol, we developed single-stranded DNA aptamers for Hodgkin lymphoma (HL) tumor cells. The aptamers specifically bound to HL cells with a high affinity, reaching maximal cell binding at 10 nM final concentration. Importantly, the aptamers were able to selectively detect HL cells and did not react to other tumor or blood cells in mixed samples, indicating that the aptamers can be used as a specific probe for in vitro analysis of HL cells. Moreover, due to the inherent properties of DNA, the aptamers were stable in human serum, suggesting potential for in vivo detection of HL tumor cells.
Department of Pathology and Genomic Medicine, Houston Methodist Hospital, and Cancer Pathology Research Laboratory, Houston Methodist Research Institute, Houston, TX 77030, USA. yzu@HoustonMethodist.org.
Parag
P
Parekh
Sanchit
S
Kamble
Nianxi
N
Zhao
Zihua
Z
Zeng
Jianguo
J
Wen
Bin
B
Yuan
Youli
Y
Zu
D052157:Aptamers, Nucleotide / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics*; D006689:Hodgkin Disease / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D052156:SELEX Aptamer Technique / Q000379:methods
D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded