A blueprint for cost-efficient localization microscopy.
Crystal clear: The authors introduce a miniaturized localization microscopy setup based on cost-effective components. They demonstrate its feasibility for subdiffraction resolution fluorescence imaging in resolving different cellular nanostructures. The setup can be used advantageously in practical courses for training students in super-resolution fluorescence microscopy.
Department of Biotechnology & Biophysics, Julius-Maximilians-University Würzburg, Am Hubland, 97074 Würzburg (Germany), Fax: (+49) 931-31-84509.
Thorge
T
Holm
Teresa
T
Klein
Anna
A
Löschberger
Tobias
T
Klamp
Gerd
G
Wiebusch
Sebastian
S
van de Linde
Markus
M
Sauer
D045744:Cell Line, Tumor; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000191:economics* / Q000295:instrumentation; D008856:Microscopy, Fluorescence / Q000191:economics* / Q000295:instrumentation; D012984:Software
cost-efficiency; fluorescence; laser spectroscopy; localization microscopy; super-resolution imaging