Plant regeneration from stem cortex protoplasts of a tomato hybrid.
Longitudinal sections containing cortical cells taken from stem internodes of a hybrid betweenLycopersicon esculentum andSolanum lycopersicoides were used as tissue sources for enzymatic protoplast isolation. Greenhouse and growth room-grown plants 4-8 weeks after rooting could be used as sources of donor tissue. Protoplasts from these tissues divided within 2-4 days of culture and numerous microcalli formed within 30 days. The shoot regeneration frequency of protoplast-derived calli was in the order of 60%. More than 100 regenerated plants which appear phenotypically normal have been established in soil.
Plant Research Centre, Agriculture Canada, K1A OC6, Ottawa, Ontario, Canada.
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Gleddie
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Keller
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Poysa