Pharmacokinetic study on costunolide and dehydrocostuslactone after oral administration of traditional medicine Aucklandia lappa Decne. by LC/MS/MS.
BACKGROUND
Radix Aucklandiae (RA), a well known traditional Chinese medicine, is widely used for treating various problems in digestive system. A selective and sensitive high-performance liquid chromatography coupled with mass spectrometry method was first developed and validated for simultaneous quantification of costunolide and dehydrocostuslactone in rat plasma with diazepam as internal standard after oral administration of RA extraction.


METHODS
Plasma samples were extracted via solid-phase extraction and detected by multiple-reaction monitoring mode under positive electrospray. Chromatographic separation was accomplished on an Agilent C18 column (2.1 mm × 150 mm, 5 µm), with 0.1% formic acid and acetonitrile (1:1) as the mobile phase at a flow rate of 0.5 mL/min.


RESULTS
The quantification was performed using the transitions of m/z 233/187 for costunolide, m/z 231/185 for dehydrocostuslactone and m/z 285/193 for diazepam, respectively. Calibration curves were linear over the concentration range of 0.7-769.7 ng/mL for costunolide and 0.9-956.0 ng/mL for dehydrocostuslactone. The intra-day and inter-day precisions (RSD%) for two compounds was less than 8.76% and 9.70% and the accuracy (RE%) range from 6.14% to 5.35%. The time to reach the maximum plasma concentration (Tmax) was 10.46 h for costunolide, 12.39 h dehydrocostuslactone. The elimination half-time (t1/2) of costunolide and dehydrocostuslactone was 5.54 ± 0.81 and 4.32 ± 0.71 (h). The AUC of costunolide and dehydrocostuslactone was 308.83 and 7884.51 respectively (ngh/mL).


CONCLUSIONS
It was the first report for the study of pharmacokinetic profile of costunolide and dehydrocostuslactone in rat plasma after oral administration of RA extract. These results provided a meaningful basis for better understanding the absorption of traditional medicine, RA, and provide useful scientific data for clinical application.
Department of Pharmacy, Logistics College of Chinese People's Armed Police Forces, Tianjin 300162, China.
Jingze
J
Zhang
Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard. Logistics College of Chinese People's Armed Police Forces, Tianjin 300162, China.
Xiao
X
Hu
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China. Electronic address: biochemgao@163.com.
Wenyuan
W
Gao
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
Zhuo
Z
Qu
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
Huimin
H
Guo
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
Zhen
Z
Liu
The State Key Laboratories of Pharmacodynamics and Pharmacokinetics, Tianjin 300193, China.
Changxiao
C
Liu
D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D004347:Drug Interactions; D007783:Lactones / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D008297:Male; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D051381:Rats; D017208:Rats, Wistar; D012717:Sesquiterpenes / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*
D007783:Lactones; D012717:Sesquiterpenes; C083030:dehydrocostus lactone; C002602:costunolide
HPLC–MS; Pharmacokinetic; Radix Aucklandiae; Sesquiterpene lactones