Influence of curcumin-loaded cationic liposome on anticancer activity for cervical cancer therapy.
The delivery of curcumin has been explored in the form of liposomal nanoparticles to treat various cancer cells. Since curcumin is water insoluble and an effective delivery route is through encapsulation in liposomes, which were modified with three components of DDAB, cholesterol and non-ionic surfactant. The purpose of this study was to establish a critical role of DDAB in liposomes containing curcumin at cellular response against two types of cell lines (HeLa and SiHa). Here, we demonstrate that DDAB is a potent inducer of cell uptake and cell death in both cell lines. The enhanced cell uptake was found on DDAB-containing liposome, but not on DDAB-free liposome. However, the cytotoxicity of DDAB-containing liposomes was high and needs to be optimized. The cytotoxicity of liposomal curcumin was more pronounced than free curcumin in both cells, suggesting the benefits of using nanocarrier. In addition, the anticancer efficiency and apoptosis effect of the liposomal curcumin formulations with DDAB was higher than those of DDAB-free liposomes. Therefore curcumin loaded liposomes indicate significant potential as delivery vehicles for the treatment of cervical cancers.
National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, Thailand.
Nattika
N
Saengkrit
National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, Thailand.
Somsak
S
Saesoo
National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, Thailand.
Wanwisa
W
Srinuanchai
National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, Thailand.
Sarunya
S
Phunpee
National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, Thailand. Electronic address: uracha@nanotec.or.th.
Uracha Rungsardthong
UR
Ruktanonchai
D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002412:Cations / Q000737:chemistry; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology / Q000627:therapeutic use*; D004705:Endocytosis / Q000187:drug effects; D005260:Female; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D018625:Microscopy, Atomic Force; D010316:Particle Size; D055672:Static Electricity; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000648:ultrastructure
D000970:Antineoplastic Agents; D002412:Cations; D008081:Liposomes; D003474:Curcumin
Cervical cancer; Cholesterol; Curcumin; Liposomes; Surfactant