Comparative evaluation of a new chemiluminiscent assay and an ELISA for the detection of IgM against measles.
BACKGROUND
The primary test for the laboratory confirmation of measles is immunoglobulin M (IgM) serology. It is therefore important to evaluate new commercial measles IgM immunoassays to ensure high-quality measles diagnostic testing. The purpose of this study was to evaluate the diagnostic performance of LIAISON IgM measles (DiaSorin, Saluggia, Italy), a new automated chemiluminescence immunoassay (CLIA), in comparison with enzyme immunoassay (EIA) Enzygnost (Siemens, Marburg, Germany).


METHODS
Sensitivity was examined using a panel of measles laboratory confirmed sera; specificity was addressed by testing sera from healthy populations and subjects with infections that may interfere with measles IgM serology.


RESULTS
The diagnostic performances of the two assays were very similar: both Enzygnost EIA and LIAISON CLIA performed with a sensitivity of 93.7% and 98.8%, whereas the specificity was 96.8% and 97.9%, respectively.


CONCLUSIONS
We concluded that LIAISON IgM measles can be a good alternative to the other immunoassay for laboratory measles confirmation.
Microbiology Service, Virgen de las Nieves University Hospital, Granada, Spain.
Antonio
A
Sampedro
Javier
J
Rodríguez-Granger
Cristina
C
Gómez
Ana
A
Lara
José
J
Gutierrez
Ana
A
Otero
D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008163:Luminescent Measurements / Q000379:methods*; D008457:Measles / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*
D000914:Antibodies, Viral; D007074:Immunoglobulin G
chemiluminescence; measles; serology