Bifidobacterium bifidum PRL2010 modulates the host innate immune response.
Here, we describe data obtained from transcriptome profiling of human cell lines and intestinal cells of a murine model upon exposure and colonization, respectively, with Bifidobacterium bifidum PRL2010. Significant changes were detected in the transcription of genes that are known to be involved in innate immunity. Furthermore, results from enzyme-linked immunosorbent assays (ELISAs) showed that exposure to B. bifidum PRL2010 causes enhanced production of interleukin 6 (IL-6) and IL-8 cytokines, presumably through NF-κB activation. The obtained global transcription profiles strongly suggest that Bifidobacterium bifidum PRL2010 modulates the innate immune response of the host.
Alimentary Pharmabiotic Centre and Department of Microbiology, Bioscience Institute, National University of Ireland, Cork, Ireland.
Francesca
F
Turroni
Valentina
V
Taverniti
Patricia
P
Ruas-Madiedo
Sabrina
S
Duranti
Simone
S
Guglielmetti
Gabriele Andrea
GA
Lugli
Laura
L
Gioiosa
Paola
P
Palanza
Abelardo
A
Margolles
Douwe
D
van Sinderen
Marco
M
Ventura
D000818:Animals; D001644:Bifidobacterium / Q000502:physiology*; D018938:Caco-2 Cells / Q000382:microbiology; D002460:Cell Line; D016207:Cytokines / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020869:Gene Expression Profiling; D019073:HT29 Cells / Q000187:drug effects / Q000382:microbiology; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D016209:Interleukin-8 / Q000378:metabolism; D007422:Intestines / Q000166:cytology / Q000276:immunology* / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D019936:Probiotics / Q000494:pharmacology*
D016207:Cytokines; D016209:Interleukin-8; D016328:NF-kappa B