Molecular cloning and multifunctional characterization of GRIM-19 (gene associated with retinoid-interferon-induced mortality 19) homologue from turbot (Scophthalmus maximus).
GRIM-19 (gene associated with retinoid-interferon-induced mortality 19), a novel cell death regulatory gene, plays important roles in cell apoptosis, embryogenesis, mitochondrial respiratory chain and immune response. To date, little information is known about fish GRIM-19 characteristics except orange-spotted grouper (Epinephelus coioides). Here a new GRIM-19 gene is identified and characterized from turbot (Scophthalmus maximus), an economic marine fish in China and Europe. Briefly, turbot GRIM-19 is a 595-bp gene encoding a 144 amino acids protein, which shares the closest relationship with Atlantic halibut (Hippoglossus hippoglossus). The expression of turbot grim-19 in liver, spleen and kidney is up-regulated by the infection of Vibrio anguillarum and LCDV (lymphocystis disease virus). Subsequently, a recombinant protein of turbot GRIM-19 is acquired and the anti-bacterial function is proved by liquid culture inhibition experiment. The subcellular location indicates that turbot GRIM-19 is co-localized with STAT3 in the cytoplasm, which is mainly determined by GRIM-19 41-84 amino acids and STAT3 1-321 amino acids. Finally, the involvements of turbot GRIM-19 in cell apoptosis and NF-κB pathway are investigated. All these data help to understand GRIM-19 function in fish, as well as provide the application possibility of GRIM-19 in fish disease resistance breeding.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Na
N
Wang
Sarite Center for Stem Cell Engineering Translational Medicine, East Hospital, Stem Cell Research Center, Tongji University School of Medicine, Shanghai 200120, China.
Xianli
X
Wang
Key Laboratory of Freshwater Biodiversity Conservation and Utilization of Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
Changgeng
C
Yang
Weifang Marine Environment Monitoring Central Station of State Oceanic Administration, Weifang 261041, China.
Xiaojie
X
Zhao
Qingdao Agricultural University, Qingdao 266109, China.
Yuxi
Y
Zhang
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Tianzi
T
Wang
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China. Electronic address: chensl@ysfri.ac.cn.
Songlin
S
Chen
D000595:Amino Acid Sequence; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004266:DNA Virus Infections / Q000276:immunology*; D029941:Fish Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005412:Flatfishes / Q000276:immunology*; D005432:Flounder / Q000276:immunology; D006801:Humans; D007497:Iridoviridae / Q000276:immunology*; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics; D016328:NF-kappa B / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D019076:Transgenes / Q000235:genetics; D015854:Up-Regulation; D014733:Vibrio / Q000276:immunology*; D014735:Vibrio Infections / Q000276:immunology*
D051017:Apoptosis Regulatory Proteins; D029941:Fish Proteins; D016328:NF-kappa B; D050796:STAT3 Transcription Factor; D009247:NADH, NADPH Oxidoreductases; C439057:NDUFA13 protein, human
Cell apoptosis; GRIM-19; Immune response; NF-κB; STAT3; Turbot (Scophthalmus maximus)