RPS8--a new informative DNA marker for phylogeny of Babesia and Theileria parasites in China.
Piroplasmosis is a serious debilitating and sometimes fatal disease. Phylogenetic relationships within piroplasmida are complex and remain unclear. We compared the intron-exon structure and DNA sequences of the RPS8 gene from Babesia and Theileria spp. isolates in China. Similar to 18S rDNA, the 40S ribosomal protein S8 gene, RPS8, including both coding and non-coding regions is a useful and novel genetic marker for defining species boundaries and for inferring phylogenies because it tends to have little intra-specific variation but considerable inter-specific difference. However, more samples are needed to verify the usefulness of the RPS8 (coding and non-coding regions) gene as a marker for the phylogenetic position and detection of most Babesia and Theileria species, particularly for some closely related species.
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Zhan-Cheng
ZC
Tian
Guang-Yuan
GY
Liu
Hong
H
Yin
Jian-Xun
JX
Luo
Gui-Quan
GQ
Guan
Jin
J
Luo
Jun-Ren
JR
Xie
Hui
H
Shen
Mei-Yuan
MY
Tian
Jin-Feng
JF
Zheng
Xiao-Song
XS
Yuan
Fang-Fang
FF
Wang
D000818:Animals; D001403:Babesia / Q000145:classification* / Q000235:genetics; D001483:Base Sequence; D002417:Cattle; D002681:China; D016054:DNA, Protozoan / Q000145:classification* / Q000235:genetics; D005091:Exons; D005819:Genetic Markers; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D015800:Protozoan Proteins / Q000145:classification* / Q000235:genetics; D012269:Ribosomal Proteins / Q000145:classification* / Q000235:genetics; D054682:Ribosome Subunits, Small, Eukaryotic / Q000737:chemistry; D016794:Theileria / Q000145:classification* / Q000235:genetics
D016054:DNA, Protozoan; D005819:Genetic Markers; D015800:Protozoan Proteins; D012269:Ribosomal Proteins