[Spectrum-effect relationship between anti-HIV 1 activities and ultra-performance liquid chromatography fingerprints of Rheum species].
OBJECTIVE
To study the relationship between ultra-performance liquid chromatography (UPLC) fingerprints of Rheum species and their anti-HIV 1 activities.


METHODS
Twenty two samples of 16 species belonging to genus Rheum from various sources were collected and analyzed in this study. Firstly they were assayed for the HIV-1 reverse transcriptase (RT) associated ribonuclease H (RNase H) activity. Secondly the fingerprints were established by an optimized UPLC method. Sample was analyzed by UPLC-TOF-MS/MS to identify major peaks. The possible relationship between UPLC fingerprints and anti-HIV 1 activities of Rheum species were deduced by mathematical statistics method.


RESULTS
Samples of R. austral, R. austral, R. hotaoense exhibited good anti-HIV 1 activities with IC50 < or = 0.2 mg x L(-1). The correlation of anti-HIV 1 activities and fingerprints showed that three compounds were the main bioactive components, and their retention times were 4.74, 7.99, 21.18 min, respectively.


CONCLUSIONS
Compounds in Rheum species with possible anti-HIV 1 activities were deduced by spectrum-effect relationship study. This study supported for study of medicinal plants in Rheum.
Chinese Academy of Medical Sciences & Peking Union Medical College, Institute of Medicinal Plant Development, Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100193, China.
Pei
P
Ma
Xin-Yao
XY
Zhang
Li-Jia
LJ
Xu
Zhe
Z
Wang
Pei-Gen
PG
Xiao
D019380:Anti-HIV Agents / Q000737:chemistry* / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology*; D054303:HIV Reverse Transcriptase / Q000378:metabolism; D015497:HIV-1 / Q000187:drug effects* / Q000201:enzymology; D020128:Inhibitory Concentration 50; D012250:Rheum / Q000737:chemistry*; D054309:Ribonuclease H, Human Immunodeficiency Virus / Q000378:metabolism; D013329:Structure-Activity Relationship
D019380:Anti-HIV Agents; D004365:Drugs, Chinese Herbal; D054303:HIV Reverse Transcriptase; D054309:Ribonuclease H, Human Immunodeficiency Virus