In vitro study of sequential fluconazole and caspofungin treatment against Candida albicans biofilms.
Candida albicans biofilms are generally considered to be resistant to azole antifungal agents but susceptible to echinocandins. We demonstrate that in a sequential therapy regimen, treatment with fluconazole first followed by caspofungin leads to a significant decrease of the efficacy of this echinocandin. Cellular stress responses induced by high fluconazole concentrations and mediated by Hsp90 and calcineurin play an important role in this phenomenon.
Department of Biology and South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, Texas, USA.
Semanti
S
Sarkar
Priya
P
Uppuluri
Christopher G
CG
Pierce
Jose L
JL
Lopez-Ribot
D000222:Adaptation, Physiological / Q000235:genetics; D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D019703:Calcineurin / Q000235:genetics / Q000378:metabolism; D002176:Candida albicans / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D000077336:Caspofungin; D054714:Echinocandins / Q000494:pharmacology*; D015725:Fluconazole / Q000494:pharmacology*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D055666:Lipopeptides; D008826:Microbial Sensitivity Tests; D013312:Stress, Physiological / Q000235:genetics; D013997:Time Factors
D000935:Antifungal Agents; D054714:Echinocandins; D005656:Fungal Proteins; D018841:HSP90 Heat-Shock Proteins; D055666:Lipopeptides; D015725:Fluconazole; D019703:Calcineurin; D000077336:Caspofungin