Spray drying formulation of albendazole microspheres by experimental design. In vitro-in vivo studies.
Both an experimental design and optimization techniques were carried out for the development of chitosan-pectin-carboxymethylcellulose microspheres to improve the oral absorption of albendazole as a model drug. The effect of three different factors (chitosan, pectin and carboxy methyl cellulose concentrations) was studied on five responses: yield, morphology, dissolution rate at 30 and 60 min, and encapsulation efficiency of the microspheres. During the screening phase, the factors were evaluated in order to identify those which exert a significant effect. Simultaneous multiple response optimizations were then used to find out experimental conditions where the system shows the most adequate results. The optimal conditions were found to be: chitosan concentration, 1.00% w/v, pectin concentration 0.10% w/v and carboxymethylcellulose concentration 0.20% w/v. The bioavailability of the loaded drug in the optimized microspheres was evaluated in Wistar rats which showed an area under curve (AUC) almost 10 times higher than the pure drug.
IQUIR - CONICET .
Agustina
A
García
Darío
D
Leonardi
Gisela N
GN
Piccirilli
María E
ME
Mamprin
Alejandro C
AC
Olivieri
María C
MC
Lamas
D000284:Administration, Oral; D015766:Albendazole / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D000818:Animals; D019540:Area Under Curve; D001682:Biological Availability; D002266:Carboxymethylcellulose Sodium / Q000008:administration & dosage; D002626:Chemistry, Pharmaceutical / Q000379:methods; D048271:Chitosan / Q000008:administration & dosage; D016503:Drug Delivery Systems; D004355:Drug Stability; D066298:In Vitro Techniques; D008297:Male; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010368:Pectins / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar
D010368:Pectins; D048271:Chitosan; D015766:Albendazole; D002266:Carboxymethylcellulose Sodium
Albendazole; biomaterials; microspheres; oral drug release; spray drying