Construction and application of site-specific artificial nucleases for targeted gene editing.
Artificial nucleases have developed into powerful tools for introducing precise genome modifications in a wide variety of species. In this chapter the authors provide detailed protocols for rapidly constructing zinc finger nucleases (ZFNs) and TALE nucleases (TALENs) and evaluating their activity for the targeted generation of InDels within the zebrafish genome.
Program in Gene Function & Expression, Department of Biochemistry & Molecular Pharmacology, UMass Medical School, Worcester, MA, USA.
Fatma O
FO
Kok
Ankit
A
Gupta
Nathan D
ND
Lawson
Scot A
SA
Wolfe
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D003851:Deoxyribonucleases / Q000235:genetics*; D004926:Escherichia coli; D018390:Gene Targeting / Q000379:methods*; D054643:INDEL Mutation; D008969:Molecular Sequence Data; D015202:Protein Engineering / Q000379:methods*; D013379:Substrate Specificity; D015027:Zebrafish / Q000235:genetics*; D016335:Zinc Fingers
D017931:DNA Primers; D003851:Deoxyribonucleases