Quantification of complement receptor 2 calcium signaling enhancement using flow cytometry.
The complement receptor 2 (Cr2) gene is exclusively expressed in B cells and follicular dendritic cells (FDC) in mice and in humans. CR2 is a receptor for the complement component 3 (C3) cleavage fragments C3d(g) and iC3b. On B cells CR2 acts as the B cell co-receptor in which ligand binding of CR2 effectively lowers the threshold for B cell activation. This protocol describes methods for the functional analysis of calcium signaling enhancement provided by CR2 co-receptor activity.
Department of Pathology, Division of Microbiology and Immunology, University of Utah School of Medicine, Salt Lake City, UT, USA.
Luke R
LR
Donius
John H
JH
Weis
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D020013:Calcium Signaling; D005434:Flow Cytometry / Q000379:methods; D051379:Mice; D008863:Microspheres; D017464:Receptors, Complement 3d / Q000276:immunology / Q000378:metabolism*; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D017464:Receptors, Complement 3d