Plant regeneration from mesophyll protoplasts of Williams' Bon Chretien (syn. Bartlett) pear (Pyrus communis L.).
Leaf protoplasts of axenic shoot cultures of Pyrus communis L. cv. Williams' Bon Chretien (syn. Bartlett) underwent cell wall regeneration and division to give multicellular colonies in a modified Murashige and Skoog medium which lacked ammonium ions, but supplemented with 1-naphthaleneacetic acid (NAA), 4-indole-3yl-acetic acid, 6-benzylaminopurine (BAP) and casein hydrolysate. Protoplast-derived colonies gave callus on Murashige and Skoog salts medium with NAA and BAP and exhibited shoot regeneration on half-strength Murashige and Skoog medium supplemented with 0.2 mg 1(-1) 4-indole-3yl-butyric acid, 2.0 mg 1(-1) BAP, 0.2 mg 1(-1) gibberellic acid, 50 mg 1(-1) casein hydrolysate and 10 mg 1(-1) Ca-pantothenate. Following rooting, protoplast-derived plants of pear were transferred to the glasshouse where they completed acclimatization.
Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, Nottingham, United Kingdom.
S J
SJ
Ochatt
J B
JB
Power