Within-population variation in susceptibility to Agrobacterium tumefaciens A281 in Picea abies (L.) Karst.
The purpose of this study was to investigate the genetic variation of susceptibility to Agrobacterium within a Picea abies population. Tumor formation was studied in 16 open-pollinated families belonging to a central Swedish population of Picea abies. Strain A281 of Agrobacterium tumefaciens was used to infect 3-monthold seedlings in a five-block greenhouse experiment. The analysis of variance showed strong significance for the between-family variation of tumor-formation percentages, varying from 28% to 73%. The most susceptible material will be suitable for experiments on the production of transgenic plants in vitro using disarmed Agrobacterium strains.
Department of Forest Genetics, Swedish University of Agricultural Sciences, Box 7027, S-750 07, Uppsala, Sweden.
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D
Clapham
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Ekberg
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G
Eriksson
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EE
Hood
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L
Norell