Antioxidant enzymes as defense mechanism against oxidative stress in midgut tissue and hemocytes of Bombyx mori larvae subjected to various stressors.
In this study, larvae of silkworm Bombyx mori were subjected to low temperature, hypoxia, and viral infection to evaluate stressor-mediated oxidative stress (OS) and the induction of antioxidant enzymes (AOEs). Exposure to cold, hypoxia, and nuclear polyhedral virus for 24 h resulted in a significant increase in hydrogen peroxide generation with concomitant increase in lipid peroxidation (LPO) and protein carbonyl levels in midgut and hemocytes. AOEs such as superoxide dismutase and catalase also increased significantly in both the tissues and the increased AOEs reverted to control values during recovery. Ontogenic stages of the larvae showed a diminishing ability of the tissues to overcome OS induced by the stressors. A significant increase in AOE activity during short stress period indicated a possible transitory defense mechanism to avoid OS-induced cell damage.
Department of Life Science, Bangalore University, Bangalore, India.
Ann Sandhya
AS
Micheal
Muthangi Veera Venkata
MV
Subramanyam
D000818:Animals; D012831:Bombyx / Q000201:enzymology* / Q000821:virology; D002374:Catalase / Q000378:metabolism; D015687:Cell Hypoxia / Q000502:physiology; D003080:Cold Temperature; D004064:Digestive System / Q000201:enzymology; D006434:Hemocytes / Q000201:enzymology; D006861:Hydrogen Peroxide / Q000378:metabolism; D007814:Larva; D015227:Lipid Peroxidation; D017924:Nucleopolyhedroviruses / Q000502:physiology; D018384:Oxidative Stress / Q000502:physiology*; D013482:Superoxide Dismutase / Q000378:metabolism
D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase
antioxidant enzymes; free radicals; lipid peroxidation; oxidative stress; protein carbonyl