Simulated digestion for testing the stability of edible vaccine based on Cucumber mosaic virus (CMV) chimeric particle display Hepatitis C virus (HCV) peptide.
Edible vaccines must survive digestive process and preserve the specific structure of the antigenic peptide to elicit effective immune response. The stability of a protein to digestive process can be predicted by subjecting it to the in vitro assay with simulated gastric fluid (SGF) and simulated intestinal fluid (SIF). Here, we describe the protocol of producing and using chimeric Cucumber mosaic virus (CMV) displaying Hepatitis C virus (HCV) derived peptide (R9) in double copy as an oral vaccine. Its stability after treatment with SGF and SIF and the preservation of the antigenic properties were verified by SDS-PAGE and immuno western blot techniques.
School of Agricultural, Forestry, Food and Environmental Sciences, University of Basilicata, Potenza, Italy.
Antonella
A
Vitti
Maria
M
Nuzzaci
Valentina
V
Condelli
Pasquale
P
Piazzolla
D017799:Cucumovirus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods; D006526:Hepatitis C / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D010455:Peptides / Q000235:genetics / Q000276:immunology*; D022462:Vaccines, Edible; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D014771:Virion / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification
D010455:Peptides; D022462:Vaccines, Edible; D014764:Viral Proteins; D014765:Viral Vaccines