Chronic exposure to bisphenol A can accelerate atherosclerosis in high-fat-fed apolipoprotein E knockout mice.
In epidemiological studies, there is growing concern regarding the association between human exposure to bisphenol A (BPA) and an increased risk for cardiovascular disease. Therefore, we investigated whether BPA accelerates atherosclerosis in mouse model. Apolipoprotein E knockout (ApoE(-/-)) mice were fed a high-fat and high-cholesterol diet with or without 50 μg/kg body weight/day BPA for 12 weeks. Atherosclerotic lesions of the aorta and aortic sinus were evaluated by Oil red O staining. After the 12-week BPA treatment, BPA significantly increased atherosclerotic lesions in the aortas of ApoE(-/-) mice by 1.7-fold (p = 0.03). Non-high-density lipoprotein (HDL) cholesterol levels in the BPA group were significantly higher compared to those in the control group (1,035 ± 70 vs. 484 ± 48 mg/dL, p = 0.02) although body weight and blood glucose levels were not different between groups. Human umbilical vein endothelial cells (HUVECs) were treated with 0.1-10 nM BPA but BPA did not affect HUVEC proliferation or migration. BPA could accelerate atherosclerosis in ApoE(-/-) mice, which may have resulted from an increase in non-HDL cholesterol levels.
Min Joo
MJ
Kim
Min Kyong
MK
Moon
Geun Hyung
GH
Kang
Kwan Jae
KJ
Lee
Sung Hee
SH
Choi
Soo
S
Lim
Byung-Chul
BC
Oh
Do Joon
DJ
Park
Kyong Soo
KS
Park
Hak Chul
HC
Jang
Young Joo
YJ
Park
D000818:Animals; D001011:Aorta / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D001018:Aortic Diseases / Q000097:blood / Q000139:chemically induced* / Q000235:genetics / Q000473:pathology; D001057:Apolipoproteins E / Q000172:deficiency* / Q000235:genetics; D050197:Atherosclerosis / Q000097:blood / Q000139:chemically induced* / Q000235:genetics / Q000473:pathology; D001559:Benzhydryl Compounds / Q000633:toxicity*; D015415:Biomarkers / Q000097:blood; D002478:Cells, Cultured; D002791:Cholesterol, Dietary / Q000097:blood; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D004785:Environmental Pollutants / Q000633:toxicity*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008297:Male; D018345:Mice, Knockout; D010636:Phenols / Q000633:toxicity*; D012307:Risk Factors; D013997:Time Factors; D015854:Up-Regulation; D000098826:Bisphenol A Compounds
D001057:Apolipoproteins E; D001559:Benzhydryl Compounds; D015415:Biomarkers; D002791:Cholesterol, Dietary; D004785:Environmental Pollutants; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds