Biotransformation of saponins by endophytes isolated from Panax notoginseng.
The biotransformation of the major saponins in Panax notoginseng, including the ginsenosides Rg1, Rh1, Rb1, and Re, by endophytes isolated from P. notoginseng was studied. One hundred and thirty-six endophytes were isolated and screened for their biotransformational abilities. The results showed that five of the tested endophytes were able to transform these saponins. These five strains were identified based on their ITS or 16S rDNA sequences, which revealed that they belonged to the genera Fusarium, Nodulisporium, Brevundimonas, and Bacillus genera. Ten transformed products were isolated and identified, including a new compound 6-O-[α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranosyl]-20-O-β-D-glucopyranosyldammarane-3,6,12,20,24,25-hexaol (3), and nine known compounds, compound K (1), ginsenoside F2 (2), vinaginsenoside R13 (4), vinaginsenoside R22 (5), pseudo-ginsenoside RT4 (6), (20S)-protopanaxatriol (7), ginsenoside Rg1 (8), vinaginsenoside R15 (9), and (20S)-3-O-β-D-glucopyranosyl-6-O-β-D-glucopyranosylprotopanaxatriol (10). This is the first study on the biotransformation of chemical components in P. notoginseng by endophytes isolated from the same plant.
Laboratory for Conservation and Utilization of Bio-resource, and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming 650091, P. R. China, (phone: +86-871-65032538).
Shao-Liu
SL
Luo
Li-Zhi
LZ
Dang
Jian-Fang
JF
Li
Cheng-Gang
CG
Zou
Ke-Qin
KQ
Zhang
Guo-Hong
GH
Li
D001711:Biotransformation; D060026:Endophytes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D053723:Panax notoginseng / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology*; D012503:Saponins / Q000032:analysis / Q000378:metabolism*
D012503:Saponins
Biotransformations; Endophytes; Panax notoginseng; Saponins