A holidic medium for Drosophila melanogaster.
A critical requirement for research using model organisms is a well-defined and consistent diet. There is currently no complete chemically defined (holidic) diet available for Drosophila melanogaster. We describe a holidic medium that is equal in performance to an oligidic diet optimized for adult fecundity and lifespan. This holidic diet supports development over multiple generations but at a reduced rate. Over 7 years of experiments, the holidic diet yielded more consistent experimental outcomes than did oligidic food for egg laying by females. Nutrients and drugs were more available to flies in holidic medium and, similar to dietary restriction on oligidic food, amino acid dilution increased fly lifespan. We used this holidic medium to investigate amino acid-specific effects on food-choice behavior and report that folic acid from the microbiota is sufficient for Drosophila development.
Institute of Healthy Ageing, Department of Genetics, Evolution and Environment, University College London, London, UK.
Matthew D W
MD
Piper
Medical Research Council (MRC) Centre for Developmental Neurobiology, King's College London, London, UK.
Eric
E
Blanc
Behavior and Metabolism Laboratory, Champalimaud Neuroscience Program, Champalimaud Centre for the Unknown, Lisbon, Portugal.
Ricardo
R
Leitão-Gonçalves
1] Institute of Healthy Ageing, Department of Genetics, Evolution and Environment, University College London, London, UK. [2].
Mingyao
M
Yang
Institute of Healthy Ageing, Department of Genetics, Evolution and Environment, University College London, London, UK.
Xiaoli
X
He
Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan, USA.
Nancy J
NJ
Linford
1] Institute of Healthy Ageing, Department of Genetics, Evolution and Environment, University College London, London, UK. [2] Max Planck Institute for Biology of Ageing, Köln, Germany.
Matthew P
MP
Hoddinott
Max Planck Institute for Biology of Ageing, Köln, Germany.
Corinna
C
Hopfen
Max Planck Institute for Biology of Ageing, Köln, Germany.
George A
GA
Soultoukis
Institute of Healthy Ageing, Department of Genetics, Evolution and Environment, University College London, London, UK.
Christine
C
Niemeyer
Institute of Healthy Ageing, Department of Genetics, Evolution and Environment, University College London, London, UK.
Fiona
F
Kerr
1] Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan, USA. [2] Geriatrics Center and Nathan Shock Center of Excellence in the Basic Biology of Aging, University of Michigan, Ann Arbor, Michigan, USA.
Scott D
SD
Pletcher
Behavior and Metabolism Laboratory, Champalimaud Neuroscience Program, Champalimaud Centre for the Unknown, Lisbon, Portugal.
Carlos
C
Ribeiro
1] Institute of Healthy Ageing, Department of Genetics, Evolution and Environment, University College London, London, UK. [2] Max Planck Institute for Biology of Ageing, Köln, Germany.
Linda
L
Partridge
D000596:Amino Acids / Q000737:chemistry; D000821:Animal Feed; D000818:Animals; D001522:Behavior, Animal; D002755:Choice Behavior; D004331:Drosophila melanogaster / Q000378:metabolism*; D016503:Drug Delivery Systems; D005247:Feeding Behavior; D005260:Female; D005298:Fertility; D020869:Gene Expression Profiling / Q000379:methods*; D005824:Genetics, Behavioral / Q000379:methods; D008136:Longevity; D013997:Time Factors
D000596:Amino Acids