Purification and characterization of plantaricin 163, a novel bacteriocin produced by Lactobacillus plantarum 163 isolated from traditional Chinese fermented vegetables.
Presumptive lactic acid bacteria (LAB) strains isolated from traditional Chinese fermented vegetables were screened for bacteriocin production. A novel bacteriocin-producing strain, Lactobacillus plantarum 163, was identified on the basis of its physiobiochemical characteristics and characterized by 16S rDNA sequencing. The novel bacteriocin, plantaricin 163, produced by Lb. plantarum 163 was purified by salt precipitation, gel filtration, and reverse-phase high-performance liquid chromatography (RP-HPLC). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis of plantaricin 163 revealed the molecular weight to be 3553.2 Da. The complete amino acid sequence showed VFHAYSARGNYYGNCPANWPSCRNNYKSAGGK, and no similarity to known bacteriocins was found. Plantaricin 163 was highly thermostable (20 min, 121 °C), active in the presence of acidic pH (3-5), sensitive to protease, and exhibited broad-spectrum antimicrobial activity against LAB and other tested Gram-positive and Gram-negative bacteria. The results suggest that plantaricin 163 may be employed as a biopreservative in the food industry.
College of Food Science and Technology, Nanjing Agricultural University, Key Laboratory of Food Processing and Quality Control, Ministry of Agriculture of China , No. 1 Weigang Nanjing, Jiangsu 210095, People's Republic of China.
Meizhong
M
Hu
Haizhen
H
Zhao
Chong
C
Zhang
Jiansheng
J
Yu
Zhaoxin
Z
Lu
D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology; D001430:Bacteriocins / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D056148:Chromatography, Reverse-Phase; D005285:Fermentation; D005516:Food Microbiology; D005520:Food Preservatives / Q000494:pharmacology; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D048191:Lactiplantibacillus plantarum / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014675:Vegetables
D000890:Anti-Infective Agents; D001430:Bacteriocins; D005520:Food Preservatives; C000591092:plantaricin 163, Lactobacillus plantarum