Opsonophagocytic assay.
The opsonophagocytic killing (OPK) assay is used as a correlate for protection to measure the functional capacities of vaccine-candidate-raised antibodies. This in vitro assay aids selecting promising vaccines by demonstrating whether the vaccine-induced antibodies drive efficient complement deposition and subsequent opsonophagocytic killing. Here, we describe two protocols for an OPK assay using either human-derived PMNs or cultured HL-60 cells.
Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Harvard University, Boston, MA, USA.
Markryan
M
Dwyer
Mihaela
M
Gadjeva
D000911:Antibodies, Monoclonal / Q000276:immunology; D003171:Complement Pathway, Classical; D018922:HL-60 Cells; D006801:Humans; D007963:Leukocytes, Mononuclear; D009895:Opsonin Proteins / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D009895:Opsonin Proteins