Shrinkage of pegylated and non-pegylated liposomes in serum.
An essential requisite for the design of nanodelivery systems is the ability to characterize the size, homogeneity and zeta potential of nanoparticles. Such properties can be tailored in order to create the most efficient drug delivery platforms. An important question is whether these characteristics change upon systemic injection. Here, we have studied the behavior of phosphatidylcholine/cholesterol liposomes exposed to serum proteins. The results reveal a serum-induced reduction in the size and homogeneity of both pegylated and non-pegylated liposomes, implicating the possible role of osmotic forces. In addition, changes to zeta-potential were observed upon exposing liposomes to serum. The liposomes with polyethylene glycol expressed different characteristics than their non-polymeric counterparts, suggesting the potential formation of a denser protein corona around the non-pegylated liposomes.
CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience & Technology of China, Beijing 100190, China; Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX 77030, USA. Electronic address: jvwolfram@tmhs.org.
Joy
J
Wolfram
Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX 77030, USA.
Krishna
K
Suri
Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX 77030, USA.
Yong
Y
Yang
Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX 77030, USA; MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
Jianliang
J
Shen
Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX 77030, USA; Department of Pharmacy, University G. d'Annunzio of Chieti, Pescara, 66013 Chieti, Italy.
Christian
C
Celia
Department of Health Science, University Magna Græcia of Catanzaro, Germaneto 88100, Italy.
Massimo
M
Fresta
CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience & Technology of China, Beijing 100190, China; Institute of High Energy Physics, Chinese Academy of Sciences (CAS), Beijing 100049, China.
Yuliang
Y
Zhao
Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX 77030, USA; Department of Cell and Developmental Biology, Weill Cornell Medical College, New York, NY 10065, USA.
Haifa
H
Shen
Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX 77030, USA; Department of Medicine, Weill Cornell Medical College, New York, NY 10065, USA. Electronic address: mferrari@tmhs.org.
Mauro
M
Ferrari
D002021:Buffers; D006801:Humans; D008081:Liposomes / Q000097:blood* / Q000737:chemistry*; D054118:Nanospheres; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D016062:Porosity; D044967:Serum / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D055672:Static Electricity; D013696:Temperature; D013997:Time Factors; D014867:Water
D002021:Buffers; D008081:Liposomes; D014867:Water; D011092:Polyethylene Glycols; D012822:Silicon Dioxide
Dynamic light scattering; Liposomes; Polydispersity index; Serum; Size; Zeta potential