Effects of triclosan on reproductive prarmeters and embryonic development of sea urchin, Strongylocentrotus nudus.
Triclosan (TCS, 2,4,4'-trichloro-2'-hydroxydiphenyl ether), a broad-spectrum antibacterial agent, is commonly found in the aquatic environment. In this study, we investigated TCS toxicity with pertaining to gamete viability, fertilization, and embryogenesis up to pluteus stage of the sea urchin, (Strongylocentrotus nudus). When the sperm and eggs were exposed to TCS (0-3.0 μM), the viability of sperm was significantly decreased at molarities higher than 1 μM of TCS. In addition, for exposure of 2.0 μM TCS the viability of eggs was not influenced and none of the sperm was viable. Fertilization rate was significantly decreased when sperm were exposed to 0.5 and 1 μM of TCS (p<0.001) and no fertilization was observed for the exposure of 1.5 μM of TCS. In embryonic development, embryos are treated with higher than 1.0 μM levels of TCS displayed arrested development. For TCS, the EC50 and LOECs values were 1.8, 1.49 and 0.99 μM and 0.53, 0.62 and 0.39 μM for sperm viability, fertilization rate, and larval development to pluteus, respectively. In the recovery test regarding normal development of arrested embryos based upon TCS exposure time, it was observed that embryos exposed to 1 μM TCS for 15 h were normally recovered for normal development, while embryos with more than 30 h exposure were not recovered to normal larvae. Overall, the results of this study strongly suggest that the gametes and embryos of S. nudus can provide the basis for an effective bioassay, with a fast and sensitive means of evaluating TCS contamination in the marine ecosystem.
South Sea Environment Research Department, Korea Institute of Ocean Science & Technology, Geoje 656-830, Republic of Korea.
Jinik
J
Hwang
South Sea Environment Research Department, Korea Institute of Ocean Science & Technology, Geoje 656-830, Republic of Korea.
Sung-Suk
SS
Suh
Marine Ecosystem Research Division, Korea Institute of Ocean Science & Technology, Ansan 426-744, Republic of Korea.
Man
M
Chang
Risk Assessment Division, National Institute of Environmental Research, Incheon 404-708, Republic of Korea.
So
S
Yun Park
Medical Research Center of Neural Dysfunction, Gyeonsang National University, Jinju 660-751, Republic of Korea.
Tae Kwon
TK
Ryu
Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Sukchan
S
Lee
South Sea Environment Research Department, Korea Institute of Ocean Science & Technology, Geoje 656-830, Republic of Korea. Electronic address: tklee@kiost.ac.
Taek-Kyun
TK
Lee
D000818:Animals; D004625:Embryo, Nonmammalian / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects; D005260:Female; D005306:Fertilization / Q000187:drug effects; D007814:Larva / Q000187:drug effects; D008297:Male; D012098:Reproduction / Q000187:drug effects; D012617:Sea Urchins / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects; D014260:Triclosan / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014874:Water Pollutants, Chemical; D014260:Triclosan
Early embryonic development; Fertilization; Sea urchin; Triclosan; Viability