The -174G/C and -572G/C interleukin 6 promoter gene polymorphisms in mexican patients with rheumatoid arthritis: a case-control study.
OBJECTIVE
There is a lack of information about the genotype frequencies of IL-6 -174G/C and -572G/C polymorphisms in Mexicans with rheumatoid arthritis (RA). Therefore, the aim of this study was to evaluate the association of the IL-6 -174G/C and -572G/C polymorphisms in Mexican mestizo with RA.


METHODS
We included 137 patients with RA and 102 healthy controls. Patients were assessed for clinical characteristics. IL-6 -174G/C and -572G/C polymorphisms were genotyped using PCR-RFLP analysis. Allele and genotype frequencies and the Hardy-Weinberg equilibrium were computed. Odds ratios (ORs) were computed to identify the risk for RA associated with the presence of GG genotype in comparison with the GC or CC genotypes.


RESULTS
The genotype -174GG occurred at a higher frequency in cases and controls (77.4% versus 78.4%, P = 0.845). We found similar results for the genotype -572GG (54% in patients versus 60.8% in controls, P = 0.295).


CONCLUSIONS
This is the first study to evaluate the association of -174G/C and -572G/C polymorphisms of the IL-6 gene with RA in Mexican mestizo patients. These two polymorphisms were not associated with RA in the studied sample. Additional studies are required to evaluate if these IL-6 polymorphisms have relevance to the development of more severe disease.
Unidad de Investigación en Epidemiología Clínica, Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro Social (IMSS), Hospital de Especialidades, 44340 Guadalajara, Mexico ; Doctorado en Ciencias Biomédicas, Orientación en Inmunología, Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara, 44340 Guadalajara, Mexico.
S A
SA
Zavaleta-Muñiz
B T
BT
Martín-Márquez
L
L
Gonzalez-Lopez
N G
NG
Gonzalez-Montoya
M L
ML
Díaz-Toscano
J M
JM
Ponce-Guarneros
A J
AJ
Ruiz-Padilla
M
M
Vázquez-Del Mercado
M
M
Maldonado-González
M
M
Fafutis-Morris
S E
SE
Flores-Martínez
E A
EA
Martínez-García
J I
JI
Gamez-Nava
D000328:Adult; D000483:Alleles; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D015850:Interleukin-6 / Q000097:blood / Q000235:genetics*; D008297:Male; D008800:Mexico; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic
D015850:Interleukin-6