Use of membrane filters for the enumeration of autotrophic thiobacilli.
A new membrane filter technique for field use was developed for the enumeration of either aerobic or anaerobic, autotrophic, sulfur-oxidizing bacteria in waters and soils. Immediately after collection, samples were filtered through sulfur-coated filters and incubated in selective media. Acidification or gas evolution was used as a growth indicator of aerobic and anaerobic thiobacilli, respectively, and related to the initial number of cells deposited on the filter.
Laboratory of Soil Microbiology, O.R.S.T.O.M., Dakar, Senegal.
M
M
Mouraret
J
J
Baldensperger