Titanium-adhesive polymer nanoparticles as a surface-releasing system of dual osteogenic growth factors.
A titanium (Ti)-adhesive nanoparticle is developed as a surface-releasing system for dual osteogenic growth factors. The Ti-adhesive nanoparticle is prepared by self-assembly of a poly(L-lactide-co-glycolide) (PLGA)-grafted hyaluronic acid (HA) copolymer, followed by conjugation of catechol groups on nanoparticle surfaces. The nanoparticles consist of Ti-adhesive peripheral catechol groups, anionic HA shells, and hydrophobic PLGA inner cores. The immobilization of the nanoparticles onto Ti substrates is successfully verified using various analytical tools including field-emission scanning electron microscopy (Fe-SEM), contact angle measurement, and X-ray photoelectron spectroscopy (XPS). Positively charged dual growth factors, bone morphogenetic protein-2 (BMP-2) and insulin-like growth factor-1 (IGF-1) are readily loaded onto the negatively charged HA shells of surface-immobilized nanoparticles, which is confirmed by fluorescence microscopy. The Ti substrates with dual growth factor-loaded nanoparticle-immobilized nanoparticles remarkably promote the attachment, proliferation, spreading, and alkaline phosphatase (ALP) activity of human adipose-derived stem cells (hADSCs).
Department of Maxillofacial Biomedical Engineering, Institute of Oral Biology, School of Dentistry, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.
Gi Hyun
GH
Choi
Hong Jae
HJ
Lee
Sang Cheon
SC
Lee
D000269:Adhesives / Q000737:chemistry*; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D049109:Cell Proliferation / Q000235:genetics*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry / Q000627:therapeutic use; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D019344:Lactic Acid / Q000737:chemistry / Q000627:therapeutic use; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D010006:Osteoblasts / Q000648:ultrastructure; D011100:Polyglycolic Acid / Q000737:chemistry / Q000627:therapeutic use; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry
D000269:Adhesives; C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D007334:Insulin-Like Growth Factor I; D006820:Hyaluronic Acid; D014025:Titanium
adhesive nanoparticles; bone morphogenetic protein-2; controlled release; insulin-like growth factor-1; surface treatment