Liquid chromatography tandem mass spectrometry for measuring ¹³C-labeling in intermediates of the glycolysis and pentose phosphate pathway.
This chapter describes a procedure to analyze (13)C-labeled phosphorylated compounds by liquid chromatography tandem mass spectrometry. Phosphorylated compounds, intermediaries of the glycolysis and pentose phosphate pathway, are separated by anion exchange chromatography and their isotopic labeling is determined by mass spectrometry. A sensitivity in the fmole range is achieved using scheduled multiple reaction monitoring mode.
Center for Applied Plant Sciences, Department of Molecular Genetics, The Ohio State University, Columbus, OH, USA.
Jean-Christophe
JC
Cocuron
Ana Paula
AP
Alonso
D002247:Carbon Isotopes; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006019:Glycolysis; D007553:Isotope Labeling; D064688:Metabolic Flux Analysis; D055432:Metabolomics / Q000379:methods; D010427:Pentose Phosphate Pathway; D010944:Plants / Q000737:chemistry / Q000378:metabolism; D053719:Tandem Mass Spectrometry
D002247:Carbon Isotopes