Agrobacterium-mediated transformation: rice transformation.
Agrobacterium is a common soil bacterium with natural capacity for trans-kingdom transfer of genetic information by transferring its T-DNA into the eukaryotic genome. In agricultural plant biotechnology, combination of non-phytopathogenic strain of Agrobacterium tumefaciens with modified T-DNA and vir-genes in a binary vector system is the most widely utilized system for genetic improvement in diverse plant species and for gene function validation. Here we have described a highly efficient A. tumefaciens-mediated transformation system for indica and japonica rice cultivars based on an immature embryo system.
Genetic Transformation Laboratory, Plant Breeding, Genetics, and Biotechnology, International Rice Research Institute, Metro Manila, Philippines.
Inez H
IH
Slamet-Loedin
Prabhjit
P
Chadha-Mohanty
Lina
L
Torrizo
D060054:Agrobacterium / Q000235:genetics* / Q000378:metabolism; D018014:Gene Transfer Techniques; D012275:Oryza / Q000235:genetics* / Q000382:microbiology; D030821:Plants, Genetically Modified; D012639:Seeds / Q000235:genetics / Q000382:microbiology; D014170:Transformation, Genetic