Genotyping of FCN and MBL2 polymorphisms using pyrosequencing.
Pyrosequencing represents one of the most thorough methods used to analyze polymorphisms. One advantage of using pyrosequencing for genotyping is the ability to identify not only single-nucleotide polymorphisms (SNPs) but also tri-allelic variations, insertions and deletions (InDels). In contrast to most other genotyping assays the sequence surrounding the polymorphism provides an internal control making this method highly reliable.
Laboratory of Molecular Medicine, Department of Clinical Immunology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
Lea
L
Munthe-Fog
Hans Ole
HO
Madsen
Peter
P
Garred
D060005:Genotyping Techniques; D006801:Humans; D037102:Lectins / Q000235:genetics*; D037601:Mannose-Binding Lectin / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods; D000097604:Ficolins
D037102:Lectins; D037601:Mannose-Binding Lectin; D000097604:Ficolins