Rubus parvifolius L. inhibited the growth of leukemia K562 cells in vitro and in vivo.
OBJECTIVE
To determine the antiproliferative activity of Rubus parvifolius L. (RP) extract, its medicinal serum and RP total saponins (RPTS) against K562 cells in vitro and in vivo.


METHODS
Nude mice models bearing leukemia tumors were treated with different concentrations of RP extract. The size, weight and histopathological change of leukemic tumors were determined. Semi-solid agar culture and methylthiazolyl tetrazolium (MTT) assay were used to determine in vitro the inhibition of colony formation and proliferation of K562 cells respectively by different concentrations of RP medicinal serum and RPTS.


RESULTS
RP extract had a tumor inhibition rate of 84.8% when administered to mice at a dose of 1.0 g/day of crude RP root equivalent. Semi-solid agar culture of K562 cells in the presence of 20% (v/v) of RP medicinal serum and 150 mg/L RPTS demonstrated a 50.8% and 100% inhibition of the colony forming unit (CFU)-K562, respectively. The same doses of RP medicinal serum and RPTS showed a proliferation inhibition of 31.4% and 86.3%, respectively against K562 cells in MTT assay.


CONCLUSIONS
RP extract and RPTS show effective antiproliferative activity against myeloid leukemia cells in vitro and in vivo.
Division of Hematology, Hangzhou Red Cross Hospital, Hangzhou, 310000, China.
Xue-jin
XJ
Zhang
Xiao-feng
XF
Xu
Rui-lan
RL
Gao
Jian-feng
JF
Xu
D000362:Agar; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000188:drug therapy / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D027824:Rosaceae / Q000737:chemistry*; D012503:Saponins / Q000494:pharmacology / Q000627:therapeutic use; D040521:Subcutaneous Tissue / Q000473:pathology; D014410:Tumor Stem Cell Assay; D023041:Xenograft Model Antitumor Assays
D010936:Plant Extracts; D012503:Saponins; D000362:Agar