Evaluation of systemic levels of neutrophilic enzymes in patients with hypertension and chronic periodontitis.
BACKGROUND
Inflammation stimulates neutrophils to release their enzymes into the extracellular matrix. The aim of the present study is to investigate the serum levels of matrix metalloproteinase (MMP)-8, MMP-9, tissue inhibitor of MMP (TIMP)-1, myeloperoxidase (MPO), and neutrophil elastase (NE) in patients with hypertension and chronic periodontitis (CP).


METHODS
A total of 95 patients were included in the study. Patients were categorized into three groups: healthy control (n = 29), hypertensive control (n = 32), and hypertensive CP (n = 34). Periodontal parameters were recorded, and serum samples were collected from each participant. Serum MMP-8, MMP-9, TIMP-1, MPO, and NE levels in circulation were assessed by enzyme-linked immunosorbent assay.


RESULTS
The hypertensive CP group had significantly higher serum MMP-8, MMP-9, and NE levels than the healthy control group (P <0.05). All study groups had similar serum TIMP-1 levels (P >0.05). Significantly higher serum MPO levels were detected in patients with hypertension and CP than healthy controls and hypertensive controls (P <0.05); however, the difference in serum MPO levels was not significant between the healthy controls and hypertensive controls (P >0.05). There was no significant difference in MMP-8/TIMP-1 ratio among the study groups (P >0.05). MMP-9/TIMP-1 ratio was significantly higher in patients with hypertension and CP than healthy controls (P <0.05).


CONCLUSIONS
The presence of hypertension along with CP has a considerable effect on serum neutrophilic enzyme levels, except TIMP-1. However, the levels of these enzymes do not seem to be affected by the presence of hypertension only. Further studies including patients who have only CP might help illuminate the effect of CP on these enzymes in patients with hypertension.
Department of Periodontology, School of Dentistry, Ege University, Ỉzmir, Turkey.
Oya
O
Türkoğlu
Nezihi
N
Barış
Taina
T
Tervahartiala
Ömer
Ö
Şenarslan
Timo
T
Sorsa
Gül
G
Atilla
D000328:Adult; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000097:blood* / Q000201:enzymology; D003774:Dental Plaque Index; D005260:Female; D006801:Humans; D006973:Hypertension / Q000097:blood* / Q000201:enzymology; D019272:Leukocyte Elastase / Q000097:blood; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000097:blood; D020780:Matrix Metalloproteinase 9 / Q000097:blood; D008875:Middle Aged; D009504:Neutrophils / Q000201:enzymology*; D017622:Periodontal Attachment Loss / Q000097:blood / Q000201:enzymology; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000097:blood / Q000201:enzymology; D009195:Peroxidase / Q000097:blood; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000097:blood
C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D009195:Peroxidase; D019272:Leukocyte Elastase; C488762:MMP8 protein, human; D020784:Matrix Metalloproteinase 8; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
Inflammation; hypertension; matrix metalloproteinases; neutrophils; periodontal diseases; tissue inhibitor of metalloproteinases