Digestive enzymes activity in subsequent generations of Cameraria ohridella larvae harvested from horse chestnut trees after treatment with imidacloprid.
In the present study we describe the effect of chloronicotinoid pesticide (imidacloprid) on the digestive enzymes activity of the Cameraria ohridella larvae after lasting 1 year sublethal exposure to imidacloprid pesticide. Caterpillars - L4 stage (fourth instar, hyperphagic tissue-feeding phase) - were collected from chemically protected white horse chestnut trees 1 year after imidacloprid treatment, and compared with caterpillars collected from non-treated trees in a previous study. Enzymes activity of α-amylase, disaccharidases, glycosidases and proteases was assayed. The presence of pesticide in ingested food changed the digestive enzymes profile of caterpillars. The analysis of correlations between different digestive enzymes showed many significant correlations (P<0.05) among glycolytic activities like β-glucosidase and α-galactosidase activities. Statistically significant correlations for proteolytic activity were found between trypsin and chymotrypsin activity and aminopeptidase activity that occurred only in the 1st generation. PCA distinguished five primary components with eigenvalues higher than 1, from which the first two explain almost 59% of analyzed results. Surprisingly, in the pesticide treated groups significantly higher activities of sucrase and lactase in relation to control were found. In general, glycosidase (α-glucosidase, β-glucosidase and β-galactosidase) activities showed a similar pattern of activity in different generations. These results contrast with those obtained with control larvae, where significant differences in activities of α-glucosidase, β-glucosidase and β-galactosidase may result from the different quantity and quality food intake by subsequent generations of larvae. No inter-generation differences in total proteolytic activity were observed in treated larvae. The absolute value of total proteolytic activity was higher than that in the control group. The pesticide present in the vascular system of the horse chestnut tree significantly affected some of the digestive enzymes activities and - in consequence - also interrelationships between enzymes, what may affect the food digestion.
Department of Physiology in Zabrze, Medical University of Silesia in Katowice, ul. Jordana 19, PL 41-808 Zabrze, Poland. Electronic address: dstygar@sum.edu.pl.
Dominika
D
Stygar
Katarzyna
K
Michalczyk
Bogdan
B
Dolezych
Miroslaw
M
Nakonieczny
Pawel
P
Migula
Maria
M
Zaak
Tomasz
T
Sawczyn
Iwona
I
Karcz-Socha
Michal
M
Kukla
Krystyna
K
Zwirska-Korczala
Rafal
R
Buldak
D031319:Aesculus / Q000469:parasitology*; D000818:Animals; D004064:Digestive System / Q000201:enzymology; D007093:Imidazoles / Q000494:pharmacology*; D019476:Insect Proteins / Q000378:metabolism*; D007814:Larva / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D009036:Moths / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000494:pharmacology*; D000516:alpha-Amylases / Q000378:metabolism; D000519:alpha-Galactosidase / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D007093:Imidazoles; D019476:Insect Proteins; D000073943:Neonicotinoids; D009574:Nitro Compounds; C082359:imidacloprid; D000516:alpha-Amylases; D000520:alpha-Glucosidases; D000519:alpha-Galactosidase; D001616:beta-Galactosidase