Parallel segmented outlet flow high performance liquid chromatography with multiplexed detection.
We describe a new approach to multiplex detection for HPLC, exploiting parallel segmented outlet flow - a new column technology that provides pressure-regulated control of eluate flow through multiple outlet channels, which minimises the additional dead volume associated with conventional post-column flow splitting. Using three detectors: one UV-absorbance and two chemiluminescence systems (tris(2,2'-bipyridine)ruthenium(III) and permanganate), we examine the relative responses for six opium poppy (Papaver somniferum) alkaloids under conventional and multiplexed conditions, where approximately 30% of the eluate was distributed to each detector and the remaining solution directed to a collection vessel. The parallel segmented outlet flow mode of operation offers advantages in terms of solvent consumption, waste generation, total analysis time and solute band volume when applying multiple detectors to HPLC, but the manner in which each detection system is influenced by changes in solute concentration and solution flow rates must be carefully considered.
Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, University of Western Sydney (Parramatta), Sydney, NSW, Australia.
Michelle
M
Camenzuli
Jessica M
JM
Terry
R Andrew
RA
Shalliker
Xavier A
XA
Conlan
Neil W
NW
Barnett
Paul S
PS
Francis
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D004867:Equipment Design; D057230:Limit of Detection; D008163:Luminescent Measurements; D017895:Manganese Compounds / Q000737:chemistry; D053610:Opiate Alkaloids / Q000032:analysis*; D009942:Organometallic Compounds / Q000737:chemistry; D010087:Oxides / Q000737:chemistry; D010207:Papaver / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D017895:Manganese Compounds; D053610:Opiate Alkaloids; D009942:Organometallic Compounds; D010087:Oxides; C048856:permanganic acid; C058901:tris(2,2'-bipyridine)ruthenium III; D015082:2,2'-Dipyridyl
Chemiluminescence detection; High performance liquid chromatography; Opiate alkaloids; Parallel segmented outlet flow