Anti-oxidizing effect of the dichloromethane and hexane fractions from Orostachys japonicus in LPS-stimulated RAW 264.7 cells via upregulation of Nrf2 expression and activation of MAPK signaling pathway.
Orostachys japonicus shows various biological activities. However, the molecular mechanisms remain unknown in LPS-stimulated macrophages. Here, we investigated the anti-oxidizing effect of the dichloromethane (DCM) and hexane fractions from O. japonicus (OJD and OJH) against oxidative stress in RAW 264.7 cells stimulated by LPS. OJD and OJH significantly increased the expression of heme oxygenase-1 (HO-1) in a dose- and time-dependent manner. Additionally, it was found that the expression of HO-1 was stimulated by Nrf2 activated via degradation of Keap1. ERK and p38 inhibitors repressed HO-1 induced by OJD and OJH in LPS-stimulated cells, respectively. In conclusion, these results suggest that OJD and OJH may block oxidative damage stimulated by LPS, via increasing the expression of HO-1 and Nrf2, and MAPK signaling pathway.
Departments of Smart Foods and Drugs, Inje University, Gimhae 621-749, Korea; Biomedical Laboratory Science, Inje University, Gimhae 621-749, Korea.
Hyeong-Seon
HS
Lee
Departments of Smart Foods and Drugs, Inje University, Gimhae 621-749, Korea; Biomedical Laboratory Science, Inje University, Gimhae 621-749, Korea.
Gyeong-Seon
GS
Lee
Departments of Smart Foods and Drugs, Inje University, Gimhae 621-749, Korea; Biomedical Laboratory Science, Inje University, Gimhae 621-749, Korea.
Seon-Hee
SH
Kim
Department of Biomedical Laboratory Science, College of Natural Science, Gimcheon University, Gimcheon 740-704, Korea.
Hyun-Kyung
HK
Kim
Departments of Smart Foods and Drugs, Inje University, Gimhae 621-749, Korea; Biomedical Laboratory Science, Inje University, Gimhae 621-749, Korea; Department of Laboratory Medicine, Inje University Busan Paik Hospital, Busan 614-735, Korea.
Dong-Hee
DH
Suk
Departments of Smart Foods and Drugs, Inje University, Gimhae 621-749, Korea; Biomedical Laboratory Science, Inje University, Gimhae 621-749, Korea.
Dong-Seok
DS
Lee
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D029627:Crassulaceae / Q000737:chemistry* / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000378:metabolism; D006586:Hexanes / Q000737:chemistry; D000072019:Kelch-Like ECH-Associated Protein 1; D008070:Lipopolysaccharides / Q000633:toxicity; D008752:Methylene Chloride / Q000737:chemistry; D051379:Mice; D020929:Mitogen-Activated Protein Kinase Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D000975:Antioxidants; D003598:Cytoskeletal Proteins; D006586:Hexanes; C117301:Keap1 protein, mouse; D000072019:Kelch-Like ECH-Associated Protein 1; D008070:Lipopolysaccharides; D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse; D010936:Plant Extracts; C026385:n-hexane; D008752:Methylene Chloride; D051547:Heme Oxygenase-1; D048051:p38 Mitogen-Activated Protein Kinases; D020929:Mitogen-Activated Protein Kinase Kinases