Expression of chicken CTCF gene in COS-1 cells and partial purification of CTCF protein.
The chicken gene for transcription factor CTCF was expressed in COS-1 mammalian cells. The CTCF protein containing polyhistidine tag was partially purified using metallo-affinity and ion-exchange chromatography. The expressed protein localized in the cell nucleus and was shown to be functionally active in the electrophoretic mobility shift assay and specifically interacted with anti-CTCF antibodies.
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia. akser@ibch.ru.
E S
ES
Kotova
I V
IV
Sorokina
S B
SB
Akopov
L G
LG
Nikolaev
E D
ED
Sverdlov
D000818:Animals; D000076246:CCCTC-Binding Factor; D019556:COS Cells; D002467:Cell Nucleus / Q000378:metabolism; D002645:Chickens; D002522:Chlorocebus aethiops; D015870:Gene Expression; D012097:Repressor Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D000076246:CCCTC-Binding Factor; D012097:Repressor Proteins