Regulation of glucose transporter expression in human intestinal Caco-2 cells following exposure to an anthocyanin-rich berry extract.
Polyphenols contained within plant tissues are consumed in significant amounts in the human diet and are known to influence a number of biological processes. This study investigated the effects of an anthocyanin-rich berry-extract on glucose uptake by human intestinal Caco-2 cells. Acute exposure (15 min) to berry extract (0.125%, w/v) significantly decreased both sodium-dependent (Total uptake) and sodium-independent (facilitated uptake) ³H-D-glucose uptake. In longer-term studies, SGLT1 mRNA and GLUT2 mRNA expression were reduced significantly. Polyphenols are known to interact directly with glucose transporters to regulate the rate of glucose absorption. Our in vitro data support this mechanism and also suggest that berry flavonoids may modulate post-prandial glycaemia by decreasing glucose transporter expression. Further studies are warranted to investigate the longer term effects of berry flavonoids on the management of glycaemia in human volunteers.
Diabetes and Nutritional Sciences Division, King's College London, London, United Kingdom.
Fawaz
F
Alzaid
Hoi-Man
HM
Cheung
Victor R
VR
Preedy
Paul A
PA
Sharp
D000872:Anthocyanins / Q000494:pharmacology*; D001692:Biological Transport; D018938:Caco-2 Cells; D015536:Down-Regulation; D004353:Drug Evaluation, Preclinical; D031985:Fragaria / Q000737:chemistry; D005638:Fruit / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D051273:Glucose Transporter Type 2 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D027923:Sambucus / Q000737:chemistry; D051269:Sodium-Glucose Transporter 1 / Q000235:genetics / Q000378:metabolism*; D029796:Vaccinium / Q000737:chemistry
D000872:Anthocyanins; D051273:Glucose Transporter Type 2; D007004:Hypoglycemic Agents; D010936:Plant Extracts; C495680:SLC2A2 protein, human; C495674:SLC5A1 protein, human; D051269:Sodium-Glucose Transporter 1; D005947:Glucose