Effects of polymorphisms and haplotypes within the MSTN gene on duck growth trait.
Abstract 1. Polymorphisms of the duck MSTN gene were investigated in 413 individuals by DNA sequencing and polymerase chain reaction restriction fragment length polymorphism. Four single nucleotide polymorphisms (G129A, C324T, A981G and C1002A), with A981G and C1002A completely linked, were found in the coding region. 2. Association analysis showed that different genotypes of all the identified SNPs were significantly associated with duck growth rate from week 5, 6 and 2 for G129A, C324T and A981G (C1002A), respectively. The greatest difference in body weight was 180 g at week 9, 106 g at week 8 and 123 g at week 8, respectively, for the three SNP's. 3. Linkage disequilibrium (LD) analysis indicated that C324T, A981G and C1002A were in strong LD. Nine main diplotypes from the reconstructed five main haplotypes were observed, and different diplotypes were significantly associated with growth rate from week 1. Birds with the h1h1 diplotype exhibited the largest body weight from week 1 onwards. 4. It was concluded that the duck MSTN gene was associated with body weight and is an important candidate gene for duck growth. traits and marker-assisted selection.
a Wuhan Institute of Animal Husbandry and Veterinary Science , Wuhan Academy of Agricultural Science & Technology , 430208 Wuhan , Hubei , P.R. China.
P
P
Gong
Y P
YP
Yang
Y
Y
Yang
S Q
SQ
Ye
B
B
Deng
L X
LX
Wang
T
T
Yu
Y G
YG
Qian
Y Z
YZ
Gong
D000818:Animals; D001483:Base Sequence; D004372:Ducks / Q000235:genetics* / Q000254:growth & development*; D005260:Female; D006239:Haplotypes; D015810:Linkage Disequilibrium; D008297:Male; D008969:Molecular Sequence Data; D055435:Myostatin / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational
D055435:Myostatin