Prediction of anaerobic biodegradability and bioaccessibility of municipal sludge by coupling sequential extractions with fluorescence spectroscopy: towards ADM1 variables characterization.
Advanced dynamic anaerobic digestion models, such as ADM1, require both detailed organic matter characterisation and intimate knowledge of the involved metabolic pathways. In the current study, a methodology for municipal sludge characterization is investigated to describe two key parameters: biodegradability and bioaccessibility of organic matter. The methodology is based on coupling sequential chemical extractions with 3D fluorescence spectroscopy. The use of increasingly strong solvents reveals different levels of organic matter accessibility and the spectroscopy measurement leads to a detailed characterisation of the organic matter. The results obtained from testing 52 municipal sludge samples (primary, secondary, digested and thermally treated) showed a successful correlation with sludge biodegradability and bioaccessibility. The two parameters, traditionally obtained through the biochemical methane potential (BMP) lab tests, are now obtain in only 5 days compared to the 30-60 days usually required. Experimental data, obtained from two different laboratory scale reactors, were used to validate the ADM1 model. The proposed approach showed a strong application potential for reactor design and advanced control of anaerobic digestion processes.
Veolia Environment Research & Innovation, Chemin de la Digue, BP 76, 78603 Maisons Laffitte Cedex, France. Electronic address: julie.jimenez@hotmail.com.
Julie
J
Jimenez
Veolia Environment Research & Innovation, Chemin de la Digue, BP 76, 78603 Maisons Laffitte Cedex, France.
Estelle
E
Gonidec
Veolia Environment Research & Innovation, Chemin de la Digue, BP 76, 78603 Maisons Laffitte Cedex, France.
Jesús Andrés
JA
Cacho Rivero
INRA, UR050, Laboratoire de Biotechnologie de l'Environnement, Av. des Etangs, Narbonne F-11100, France.
Eric
E
Latrille
Veolia Environment Research & Innovation, Chemin de la Digue, BP 76, 78603 Maisons Laffitte Cedex, France.
Fabien
F
Vedrenne
INRA, UR050, Laboratoire de Biotechnologie de l'Environnement, Av. des Etangs, Narbonne F-11100, France.
Jean-Philippe
JP
Steyer
D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D002138:Calibration; D005591:Chemical Fractionation / Q000379:methods*; D002947:Cities; D005060:Europe; D016018:Least-Squares Analysis; D008697:Methane / Q000096:biosynthesis; D008962:Models, Theoretical; D015203:Reproducibility of Results; D012722:Sewage / Q000382:microbiology*; D013050:Spectrometry, Fluorescence
D012722:Sewage; D008697:Methane
3D fluorescence; Anaerobic digestion; Bioaccessibility; Biodegradability; Modelling; Municipal sludge; Organic matter characterization