Transformation of Vicia narbonensis via Agrobacterium-mediated gene transfer.
Shoot tips and epicotyl-segments of Vicia narbonensis were co-cultivated with Agrobacterium tumefaciens strain C58C1 pGV 3850 HPT, carrying a plasmid coding for hygromycin-phosphotransferase. On callus-induction medium containing 60 mg/l hygromycin for selection, approximately 18% of the explants produced hygromycin-resistant callus. After transfer to regeneration-medium these calluses produced hygromycin-resistant and nopaline-positive somatic embryos which could be regenerated to plantlets. The integration of the T-DNA into the plant genome was confirmed by Southern analysis.
Institut für Angewandte Genetik, Freie Universität Berlin, Albrecht-Thaer-Weg 6, 33, Berlin, FRG.
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Pickardt
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Meixner
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Schade
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Schieder