Plant regeneration from protoplasts of common buckwheat (fagopyrum esculentum).
Protoplasts were isolated from hypocotyls of etiolated seedlings from a diploid and the corresponding autotetraploid variety of common buckwheat (Fagopyrum esculentum). The isolated protoplasts started to divide after 4 days in culture in a modified MS medium. Maximum plating efficiency was approximately 1%. Regenerated calli derived from the tetraploid genotype developed roots easily but were recalcitrant to form shoots. Eighteen months following the initiation of cultures, tetraploid embryoids and shoots emerged after 3 weeks on an MS medium containing 0.1 mg/l gibberellic acid.
Laboratory of Plant Breeding, Faculty of Agriculture, Miyazaki University, 889-21, Miyazaki, Japan.
T
T
Adachi
A
A
Yamaguchi
Y
Y
Miike
F
F
Hoffmann