Hypothermia induced by adenosine 5'-monophosphate attenuates injury in an L-arginine-induced acute pancreatitis rat model.
OBJECTIVE
This study sought to investigate the effects of hypothermia induced by adenosine 5'-monophosphate (5'-AMP) on L-arginine (L-Arg)-induced acute pancreatitis in rats.


METHODS
The rats were divided into four groups: the control group, the acute pancreatitis group, the 5'-AMP pretreatment group, and the 5'-AMP posttreatment group. Rats in all groups, except for the control group, received two injections of 2.5 g/kg body weight (intraperitoneally) L-Arg, with an interval of 1 h between the injections. Subsequently, the rats were observed to assess whether hypothermia induced by 5'-AMP could effectively inhibit inflammation associated with L-Arg-induced acute pancreatitis in rats.


RESULTS
Hypothermia induced by 5'-AMP produced protective effects in our acute pancreatitis model. These effects exhibited the following manifestations: (i) a significant reduction in rat mortality rates; (ii) a significant decrease in the occurrence of pancreatic edema; (iii) significant reductions in serum amylase (P < 0.001), interleukin-6 (P < 0.001), interleukin-1β (P < 0.001) and tumor necrosis factor-α (P < 0.001); (iv) the significant inhibition of nuclear factor-κB (NF-κB) activation in rats that were pre- and posttreated with 5'-AMP compared with rats that were only injected with L-Arg; and (v) significant decreases in the occurrence of pancreatic interstitial edema, inflammatory cell infiltration, hemorrhage, and acinar cell necrosis.


CONCLUSIONS
Hypothermia induced by 5'-AMP could inhibit the acute inflammatory reaction and NF-κB activation associated with acute pancreatitis.
Gout Laboratory, Shandong Provincial Key Laboratory of Metabolic Diseases, Qingdao, China.
Yunlong
Y
Wang
Weiting
W
Guo
Yuan
Y
Li
Xinting
X
Pan
Wenshan
W
Lv
Lingling
L
Cui
Changgui
C
Li
Yangang
Y
Wang
Shengli
S
Yan
Jidong
J
Zhang
Bin
B
Liu
D000208:Acute Disease; D000249:Adenosine Monophosphate / Q000008:administration & dosage / Q000627:therapeutic use*; D000681:Amylases / Q000097:blood; D000818:Animals; D001120:Arginine / Q000009:adverse effects*; D004195:Disease Models, Animal; D007036:Hypothermia, Induced; D018836:Inflammation Mediators / Q000378:metabolism; D053583:Interleukin-1beta; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D010195:Pancreatitis / Q000139:chemically induced* / Q000235:genetics / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D018836:Inflammation Mediators; D053583:Interleukin-1beta; D015850:Interleukin-6; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D000249:Adenosine Monophosphate; D001120:Arginine; D000681:Amylases
L-arginine; acute pancreatitis; adenosine 5′-monophosphate