Genotoxicity and antileishmanial activity evaluation of Physalis angulata concentrated ethanolic extract.
Antileishmanial in vitro tests, as well as Ames and micronucleus assays were performed with a concentrated ethanolic extract of Physalis angulata (EEPA) RESULTS: EEPA did not present mutagenic effect in Salmonella typhimurium strains at concentration reaching 3000 μg/plate and did not induce mutagenic effects after two oral administrations with a 24h interval at a dose level of 2000 mg/kg. EEPA presented antileishmanial activity and presented an IC₅₀ value of 5.35 ± 2.50 μg/mL and 4.50 ± 1.17 μg/mL against Leishmania amazonensis and Leishmania braziliensis promastigotes, respectively. In the cytotoxicity test against macrophages, the EEPA had a LC₅₀ of 6.14 ± 0.59 μg/mL. Importantly, the IC₅₀ against L. amazonensis intracellular amastigotes was 1.23 ± 0.11 μg/mL.


CONCLUSIONS
EEPA extract is non-mutagenic and presented a promising pharmacological effect against Leishmania parasites.
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BA, Brazil.
Renata Campos
RC
Nogueira
Vinicius Pinto Costa
VP
Rocha
Fabiana Regina
FR
Nonato
Therezinha Coelho Barbosa
TC
Tomassini
Ivone Maria
IM
Ribeiro
Ricardo Ribeiro
RR
dos Santos
Milena Botelho Pereira
MB
Soares
D000818:Animals; D000981:Antiprotozoal Agents; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol; D005260:Female; D007891:Leishmania / Q000187:drug effects*; D007892:Leishmania braziliensis / Q000187:drug effects; D007894:Leishmania mexicana / Q000187:drug effects; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009153:Mutagens; D032321:Physalis / Q000737:chemistry*; D010936:Plant Extracts / Q000633:toxicity*; D018547:Plant Stems / Q000737:chemistry; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D000981:Antiprotozoal Agents; D009153:Mutagens; D010936:Plant Extracts; D012997:Solvents; D000431:Ethanol
Ames test; Genotoxicity; Leishmaniasis; Micronucleus test; Physalis angulata L.