Genome-wide analysis and evolutionary study of sucrose non-fermenting 1-related protein kinase 2 (SnRK2) gene family members in Arabidopsis and Oryza.
The over-expression of plant specific SnRK2 gene family members by hyperosmotic stress and some by abscisic acid is well established. In this report, we have analyzed the evolution of SnRK2 gene family in different plant lineages including green algae, moss, lycophyte, dicot and monocot. Our results provide some evidences to indicate that the natural selection pressure had considerable influence on cis-regulatory promoter region and coding region of SnRK2 members in Arabidopsis and Oryza independently through time. Observed degree of sequence/motif conservation amongst SnRK2 homolog in all the analyzed plant lineages strongly supported their inclusion as members of this family. The chromosomal distributions of duplicated SnRK2 members have also been analyzed in Arabidopsis and Oryza. Massively Parallel Signature Sequencing (MPSS) database derived expression data and the presence of abiotic stress related promoter elements within the 1 kb upstream promoter region of these SnRK2 family members further strengthen the observations of previous workers. Additionally, the phylogenetic relationships of SnRK2 have been studied in all plant lineages along with their respective exon-intron structural patterns. Our results indicate that the ancestral SnRK2 gene of land plants gradually evolved by duplication and diversification and modified itself through exon-intron loss events to survive under environmental stress conditions.
Department of Biological Sciences (Section Biotechnology), Presidency University, 86/1 College Street, Kolkata 700073, India; Department of Biological Sciences (Section Botany), Presidency University, 86/1 College Street, Kolkata 700073, India.
Jayita
J
Saha
Department of Biological Sciences (Section Biotechnology), Presidency University, 86/1 College Street, Kolkata 700073, India.
Chitrita
C
Chatterjee
Department of Biological Sciences (Section Biotechnology), Presidency University, 86/1 College Street, Kolkata 700073, India; Department of Biological Sciences (Section Botany), Presidency University, 86/1 College Street, Kolkata 700073, India.
Atreyee
A
Sengupta
Department of Biological Sciences (Section Botany), Presidency University, 86/1 College Street, Kolkata 700073, India. Electronic address: kamala.botany@presiuniv.ac.in.
Kamala
K
Gupta
Department of Biological Sciences (Section Biotechnology), Presidency University, 86/1 College Street, Kolkata 700073, India. Electronic address: bhaskarzoology@gmail.com.
Bhaskar
B
Gupta
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D019295:Computational Biology; D019143:Evolution, Molecular; D018745:Genome, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D012275:Oryza / Q000235:genetics*; D010802:Phylogeny; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D016415:Sequence Alignment
D029681:Arabidopsis Proteins; C492978:SnRK2 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases
Abiotic stress; Phylogenetic tree; Promoter element; SNF1-related protein kinase 2; SnRK2