Single-molecule resolution of protein structure and interfacial dynamics on biomaterial surfaces.
A method was developed to monitor dynamic changes in protein structure and interfacial behavior on surfaces by single-molecule Förster resonance energy transfer. This method entails the incorporation of unnatural amino acids to site-specifically label proteins with single-molecule Förster resonance energy transfer probes for high-throughput dynamic fluorescence tracking microscopy on surfaces. Structural changes in the enzyme organophosphorus hydrolase (OPH) were monitored upon adsorption to fused silica (FS) surfaces in the presence of BSA on a molecule-by-molecule basis. Analysis of >30,000 individual trajectories enabled the observation of heterogeneities in the kinetics of surface-induced OPH unfolding with unprecedented resolution. In particular, two distinct pathways were observed: a majority population (∼ 85%) unfolded with a characteristic time scale of 0.10 s, and the remainder unfolded more slowly with a time scale of 0.7 s. Importantly, even after unfolding, OPH readily desorbed from FS surfaces, challenging the common notion that surface-induced unfolding leads to irreversible protein binding. This suggests that protein fouling of surfaces is a highly dynamic process because of subtle differences in the adsorption/desorption rates of folded and unfolded species. Moreover, such observations imply that surfaces may act as a source of unfolded (i.e., aggregation-prone) protein back into solution. Continuing study of other proteins and surfaces will examine whether these conclusions are general or specific to OPH in contact with FS. Ultimately, this method, which is widely applicable to virtually any protein, provides the framework to develop surfaces and surface modifications with improved biocompatibility.
Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80309.
Sean Yu
SY
McLoughlin
Mark
M
Kastantin
Daniel K
DK
Schwartz
Joel L
JL
Kaar
D000327:Adsorption; D043303:Aryldialkylphosphatase / Q000737:chemistry* / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D042063:Caulobacteraceae / Q000201:enzymology*; D031541:Fluorescence Resonance Energy Transfer; D007700:Kinetics; D008856:Microscopy, Fluorescence / Q000379:methods*; D008958:Models, Molecular; D011487:Protein Conformation; D012822:Silicon Dioxide / Q000737:chemistry / Q000378:metabolism
D001672:Biocompatible Materials; D012822:Silicon Dioxide; D043303:Aryldialkylphosphatase
protein adhesion; single-molecule fluorescence; total internal reflection fluorescence microscopy