Multiplexing enhancement for the detection of multiple pathogen DNA.
Various methods for the detection of pathogens have been researched and developed. However, most of detection methods are cost-ineffective and laborious. To minimize costs and labor, multi-detection of pathogen have been widely used. In this paper, we propose a novel multi-detection method, which can enhance multi-detection capability using fluorescent dye labeled DNA nanostructures that is named DNA nanobarcodes. By using three fluorescence colors, multi-detection capability is significantly increased because of the increased combination of three colors. Moreover, our approach uses a relatively simple DNA nanostructure to precisely control the fluorescence intensity ratio. Therefore, high multi-detection ability is achieved without constructing a complicated DNA nanostructure probe. Our novel detection method can overcome the obstacles of conventional methods and enhance multi-detection capability effectively. By using our new system, we were able to successfully detect nine different DNA pathogens simultaneously. Our system can easily increase the multiplexibility by using more fluosrescent colors on DNA nanobarcode.
Department of Chemical Engineering, University of Seoul, Seoul 130-743, South Korea.
Daehoon
D
Han
Jinkee
J
Hong
Hyun Cheol
HC
Kim
Jong Hwan
JH
Sung
Jong Bum
JB
Lee
D045424:Complex Mixtures / Q000032:analysis*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods
D045424:Complex Mixtures; D004269:DNA, Bacterial; D004271:DNA, Fungal; D004279:DNA, Viral; D005456:Fluorescent Dyes