Determination of fusaric acid in maize using molecularly imprinted SPE clean-up.
A new LC method to detect fusaric acid (FA) in maize is reported based on a molecularly imprinted SPE clean-up using mimic-templated molecularly imprinted polymers. Picolinic acid was used as a toxin analog for imprinting polymers during a thermolytic synthesis. Both acidic and basic functional monomers were predicted to have favorable binding interactions by MP2 ab initio calculations. Imprinted polymers synthesized with methacrylic acid or 2-dimethylaminoethyl methacrylate exhibited imprinting effects in SPE analysis. FA levels were determined using RP ion-pairing chromatography with diode-array UV detection and tetrabutylammonium hydrogen sulfate in the mobile phase. A method was developed to detect FA in maize using molecularly imprinted SPE analysis within the range of 1-100 μg/g with recoveries between 83.9 and 92.1%.
Bacterial Foodborne Pathogens and Mycology Research Unit, United States Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, Peoria, IL, USA.
Michael
M
Appell
Michael A
MA
Jackson
Lijuan C
LC
Wang
Che-Hin
CH
Ho
Anja
A
Mueller
D000327:Adsorption; D005506:Food Contamination / Q000032:analysis; D005669:Fusaric Acid / Q000737:chemistry / Q000302:isolation & purification*; D054802:Molecular Imprinting; D009183:Mycotoxins / Q000737:chemistry / Q000302:isolation & purification*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods; D003313:Zea mays / Q000737:chemistry*
D009183:Mycotoxins; D011108:Polymers; D005669:Fusaric Acid
Food safety; HPLC; Ion pair chromatography; Molecularly imprinted polymers; Mycotoxins