Proteolytic antigens interfere with endosome/lysosome fusion in epithelial cells.
The airway epithelial barrier function is important in maintaining the homeostasis in the body. A number of airway disorders are associated with the epithelial barrier dysfunction. The present study aims to elucidate a possible mechanism by which the proteolytic allergens compromise the epithelial barrier function. The airway epithelial cell line, RPMI 2650 cells (Rp cells) and kidney epithelial cell line, MDCK cells, were cultured to be monolayers and used as an in vitro epithelial barrier model. House dust mite antigen, Der P1 (Der) was used as an antigen that has the proteolytic property. The epithelial barrier permeability and transepithelial resistance (TER) were used as the indicators of epithelial barrier function. Both epithelial cell lines could endocytose Der in the culture. Some of the Der was transported across the epithelial barrier to the basal chambers of the Transwells without affecting the TER. The endocytic Der could suppress the expression of ubiquitin E3 lases A20 and further interfered with the fusion of endosome/lysosome in the epithelial cells. Mite antigen, Der, can interfere with the fusion of endosome/lysosome in epithelial cells to induce the epithelial barrier dysfunction.
Department of Anesthesiology, Shengjing Hospital, China Medical University, Shenyang, China.
Yu-Wei
YW
Liao
Xing-Mao
XM
Wu
Jia
J
Jia
Xiao-Lei
XL
Wu
Hong
H
Tao
Hai-Yuan
HY
Wang
D000818:Animals; D039741:Antigens, Dermatophagoides / Q000494:pharmacology*; D060829:Arthropod Proteins / Q000494:pharmacology*; D001692:Biological Transport; D002460:Cell Line; D003546:Cysteine Endopeptidases / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D004285:Dogs; D004705:Endocytosis; D011992:Endosomes / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D008247:Lysosomes / Q000187:drug effects* / Q000378:metabolism; D061985:Madin Darby Canine Kidney Cells; D008561:Membrane Fusion / Q000187:drug effects*; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D010539:Permeability; D059748:Proteolysis; D020545:Respiratory Mucosa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3
D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; C076529:TNFAIP3 protein, human; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3; D003546:Cysteine Endopeptidases; C451162:Dermatophagoides pteronyssinus antigen p 1