Subunits of tetrameric α-amylase inhibitors of Hordeum chilense are encoded by genes located in chromosomes 4H(ch) and 7H (ch.).
Three proteins (components 1, 2, and 4) of the non-prolamin, 70% ethanol soluble fraction from the endosperm of Hordeum chilense have been identified as putative subunits of the tetrameric inhibitors active against insect α-amylases. In experiments carried out with the synthetic alloploid Tritordeum (H. chilense x Triticum turgidum conv. durum), previously described proteins from T. turgidum, designated CM2, CM3 and CM 16, have been also identified as subunits of α-amylase inhibitors. Genes for components 1 and 4 of H. chilense have been located in chromosomes 4H(ch) and 7H(ch), based on the analysis of H. chilense-T.turgidum addition lines. Subunits of the inhibitors from wheat and from cultivated barley had been previously assigned to chromosomes of the same homoeology groups.
Departamento de Bioquímica, E.T.S. Ingenieros Agrónomos, Ciudad Universitaria, E-28040, Madrid, Spain.
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Sanchez-Monge
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Fernandez
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Salcedo