Exercise decreases susceptibility to homocysteine seizures: the role of oxidative stress.
The aim of the study was to examine the effects of chronic exercise training on seizures induced by homocysteine thiolactone (HCT) in adult rats. Rats were assigned to: sedentary control; exercise control; sedentary+HCT; exercise+HCT group. Animals in the exercise groups ran 30 min daily on a treadmill for 30 consecutive days (belt speed 20 m/min), while sedentary rats spent the same time on the treadmill (speed 0 m/min). On the 31st day, the HCT groups received HCT (8.0 mmol/kg), while the control groups received vehicle. Afterwards, convulsive behavior and EEG activity were registered. Lipid peroxidation, superoxide dismutase (SOD) and catalase (CAT) activity were ascertained in the rat hippocampus. No signs of seizures were registered in sedentary and exercise control rats. Seizure latency was increased, while number of seizure episodes and spike-and-wave discharges (SWD) in EEG were decreased in the exercise+HCT compared to the sedentary+HCT group. Seizure incidence, the severity thereof and duration of SWDs were not significantly different between these groups. Exercise partly prevented increase of lipid peroxidation and decrease of the SOD and CAT activity after HCT administration. These results indicate beneficial effects of exercise in model of HCT-induced seizures in rats, what could be, at least in part, a consequence of improved antioxidant enzymes activity.
Faculty of Medicine, Institute of Medical Physiology "Richard Burian", University of Belgrade, Serbia.
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Hrncic
Faculty of Medicine, Institute of Medical Physiology "Richard Burian", University of Belgrade, Serbia.
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Rasic-Markovic
Faculty of Medicine, Institute of Medical Physiology "Richard Burian", University of Belgrade, Serbia.
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Lekovic
Faculty of Medicine, Department of Medical Chemistry, University of Belgrade, Serbia.
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Krstic
Institute of Nuclear Sciences Vinca, University of Belgrade, Serbia.
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Colovic
Faculty of Medicine, Institute of Endocrinology, Diabetes and Metabolic Diseases, University of Belgrade, Serbia.
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Macut
Department of Medical Sciences, Serbian Academy of Sciences and Arts, Belgrade, Serbia.
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Susic
Faculty of Medicine, Institute of Medical Physiology "Richard Burian", University of Belgrade, Serbia.
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Djuric
Faculty of Medicine, Institute of Medical Physiology "Richard Burian", University of Belgrade, Serbia.
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Stanojlovic
D000818:Animals; D002374:Catalase / Q000378:metabolism; D004195:Disease Models, Animal; D004569:Electroencephalography; D006624:Hippocampus / Q000378:metabolism; D006710:Homocysteine / Q000031:analogs & derivatives; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D010805:Physical Conditioning, Animal; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism; D013858:Thiobarbiturates / Q000378:metabolism
D013858:Thiobarbiturates; D006710:Homocysteine; D008315:Malondialdehyde; C007957:homocysteine thiolactone; D002374:Catalase; D013482:Superoxide Dismutase; C029684:thiobarbituric acid