The case for measuring antibodies to specific citrullinated antigens.
Anti-citrullinated protein/peptide antibodies (ACPA) are the principal autoantibody system associated with rheumatoid arthritis (RA), with diagnostic sensitivity of 70% and specificity of 95%. Current testing for ACPA uses the anti-cyclic citrullinated peptide assay (anti-CCP) which measures a generalized reactivity with citrulline-containing peptides, thus giving no insight into reactivity to specific RA antigens. Of these, the best characterized are, α-enolase, fibrinogen/fibrin, vimentin, Type 2 collagen and filaggrin, antibodies to each of which are found in approximately 30-60% of RA cases. Given reports of cross-reactivity between citrullinated antigens, we discuss whether or not measuring these specific antibodies could aid: clinical diagnosis, identification of clinical subsets and drug responses, or provide insight into pathogenic mechanisms or etiology of RA.
Nuffield Department of Orthopaedics, University of Oxford, Kennedy Institute of Rheumatology, Rheumatology and Musculoskeletal Sciences, Oxford, UK.
Anna B
AB
Montgomery
Patrick J
PJ
Venables
Benjamin A
BA
Fisher
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000175:diagnosis* / Q000276:immunology; D001323:Autoantibodies / Q000097:blood*; D001324:Autoantigens / Q000737:chemistry / Q000276:immunology; D015415:Biomarkers / Q000097:blood*; D054316:Biomarkers, Pharmacological / Q000097:blood*; D002956:Citrulline / Q000737:chemistry; D003429:Cross Reactions; D000939:Epitopes; D000091344:Filaggrin Proteins; D006801:Humans; D011379:Prognosis; D012680:Sensitivity and Specificity; D012699:Serology / Q000379:methods* / Q000639:trends
D001323:Autoantibodies; D001324:Autoantigens; D015415:Biomarkers; D054316:Biomarkers, Pharmacological; D000939:Epitopes; C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D002956:Citrulline