In vitro analysis of the complete CIDE domain interactions of the  Drep system in fly.
CIDE domain containing proteins are involved in apoptosis and lipid metabolism, and four CIDE containing proteins, Drep1, Drep2, Drep3, and Drep4, have been identified in fly. In this study, we found that Drep3 interacts with Drep4 via the CIDE domain specifically, which completes the interaction map of Drep system in fly, cyclic interactions: Drep1–Drep2–Drep3–Drep4–Drep1. In addition, we analyzed the dynamic stoichiometry changes of Drep proteins upon binding to their binding partners. Our current studies will help us to understand Drep system in fly as well as CIDE domain for protein–protein interactions.
Seung Mi
SM
Lee
Hyun Ho
HH
Park
D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism*; D029721:Drosophila Proteins / Q000378:metabolism*; D008958:Models, Molecular; D011485:Protein Binding; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary
D051017:Apoptosis Regulatory Proteins; C402283:Drep3 protein, Drosophila; D029721:Drosophila Proteins; C109664:caspase-activated DNase inhibitor; D003851:Deoxyribonucleases; C402284:Drep4 protein, Drosophila