[Study on molecular identification of water extracts from Gelsemium elegans and Lonicera japonica and its close species by specific PCR amplification].
OBJECTIVE
To explore the new method of discriminating Gelsemium elegans from Lonicera japonica and its close species by using specific PCR amplification.


METHODS
Thirteen samples of the different G. elegans materials and 58 samples of L. japonica, L. macranthoides and L. dasystyla were collected. The total DNA of the samples were extracted, and the DNA of G. elegans, L. japonica and L. macranthoides water extracts were extracted. PsbA-rnnH sequence from G. elegans was amplified by PCR and sequenced unidirectionally, ClustulW was used to align psbA-trnH sequences of the G. elegans and L. japonica and its close species from GenBank database.


RESULTS
All samples were amplified by PCR with specific primer, DNA from G. elegans would be amplified 97 bp whereas PCR products from all of Lonicera samples had not bands.


CONCLUSIONS
Specific PCR amplification can be used to identify G. elegans from L. japonica and its close species successfully and is an efficient molecular marker for authentication of G. elegans and L. japonica and its close species.
National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhan-Hu
ZH
Cui
Chao
C
Jiang
Lu-Qi
LQ
Huang
Min-Hui
MH
Li
Tao
T
Zhou
Li-She
LS
Zhou
Yuan
Y
Yuan
D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D031450:Gelsemium / Q000737:chemistry* / Q000235:genetics*; D031239:Lonicera / Q000737:chemistry* / Q000235:genetics*; D010802:Phylogeny; D010936:Plant Extracts / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction; D014867:Water / Q000737:chemistry*
D018744:DNA, Plant; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D014867:Water