Solid phase microextraction fills the gap in tissue sampling protocols.
Metabolomics and biomarkers discovery are an integral part of bioanalysis. However, untargeted tissue analysis remains as the bottleneck of such studies due to the invasiveness of sample collection, as well as the laborious and time-consuming sample preparation protocols. In the current study, technology integrating in vivo sampling, sample preparation and global extraction of metabolites--solid phase microextraction was presented and evaluated during liver and lung transplantation in pig model. Sampling approaches, including selection of the probe, transportation, storage conditions and analyte coverage were discussed. The applicability of the method for metabolomics studies was demonstrated during lung transplantation experiments.
Department of Chemistry, University of Waterloo, Waterloo, ON, Canada.
Barbara
B
Bojko
Krzysztof
K
Gorynski
German Augusto
GA
Gomez-Rios
Jan Matthias
JM
Knaak
Tiago
T
Machuca
Vinzent Nikolaus
VN
Spetzler
Erasmus
E
Cudjoe
Michael
M
Hsin
Marcelo
M
Cypel
Markus
M
Selzner
Mingyao
M
Liu
Shaf
S
Keshavjee
Janusz
J
Pawliszyn
D000818:Animals; D008099:Liver / Q000378:metabolism*; D016031:Liver Transplantation; D008168:Lung / Q000378:metabolism*; D016040:Lung Transplantation; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D009926:Organ Preservation / Q000379:methods*; D052617:Solid Phase Microextraction / Q000379:methods*; D013048:Specimen Handling / Q000379:methods; D013552:Swine
In vivo SPME; Metabolomics; Surgery; Tissue sampling