Plant regeneration from cytoplasmic hybrids of rice (Oryza sativa L.).
We obtained cybrid plants by electrofusing γ-irradiated protoplasts of a cytoplasmic male-sterile line "A-58 CMS" (Oryza sativa L.) and iodoacetamide (IOA)-treated protoplasts of the fertile (normal) rice cultivar "Fujiminori". The cybridity of the plants was confirmed by mitochondrial (mt) DNA restriction endonuclease, and plasmid-like DNA analyses, and by isozyme, cytological and morphological investigations. The chromosome number of the cybrid plants is 24.
Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, Sakyo-ku, 606, Kyoto, Japan.
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Yang
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Shikanai
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Mori
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Yamada