Fabrication of Phaeodactylum tricornutum extract-loaded gelatin nanofibrous mats exhibiting antimicrobial activity.
Microalgae have recently been recognized as a valuable resource for various applications. Phaeodactylum tricornutum is a diatom that lives in marine water and has an unusually high content of lipids. In this study, we added P. tricornutum into a gelatin dope solution to examine the effect of this diatom using electrospinning. The addition of P. tricornutum extracts increased the conductivity of the dope solution but had little effect on the viscosity. Due to the increased conductivity, the fiber diameter was reduced compared with the neat gelatin nanofiber. The loading of P. tricornutum extracts was confirmed using fluorescence microscopy, and the incorporation of lipids was detected through gas chromatography. The P. tricornutum-loaded nanofiber mat exhibited anti-microbial activity against Escherichia coli and multidrug-resistant Staphylococcus aureus (MRSA). The cell viability test showed that the P. tricornutum-loaded nanofiber has no cytotoxicity. We expect that this antimicrobial P. tricornutum-loaded gelatin nanofiber mat can be applied as a wound dressing.
Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 151-921, Republic of Korea.
Hyo Won
HW
Kwak
Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 151-921, Republic of Korea.
Min Ji
MJ
Kang
Department of Marine Bio-materials and Aquaculture, Pukyong National University, Pusan 608-737, Republic of Korea.
Jin Hee
JH
Bae
Department of Marine Bio-materials and Aquaculture, Pukyong National University, Pusan 608-737, Republic of Korea.
Sung Bum
SB
Hur
Faculty of Textile Science and Technology, Nano Fusion Technology Lab, Shinshu University, Ueda, Nagano 386-8567, Japan.
Ick-Soo
IS
Kim
Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 151-921, Republic of Korea.
Young Hwan
YH
Park
Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 151-921, Republic of Korea; Center for Food & Bioconvergence, Seoul National University, Seoul 151-921, Republic of Korea; Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea. Electronic address: prolee@snu.ac.kr.
Ki Hoon
KH
Lee
D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D017377:Diatoms / Q000737:chemistry*; D018432:Drug Resistance, Multiple / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D005780:Gelatin / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D041681:NIH 3T3 Cells; D057139:Nanofibers / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics; D014945:Wound Healing / Q000187:drug effects
D000890:Anti-Infective Agents; D010936:Plant Extracts; D005780:Gelatin
Anti-microbial; Gelatin; MRSA; Microalgae; Phaeodactylum tricornutum; Wound dressing