Rhodamine B induces long nucleoplasmic bridges and other nuclear anomalies in Allium cepa root tip cells.
The cytogenetic toxicity of rhodamine B on root tip cells of Allium cepa was investigated. A. cepa were cultured in water (negative control), 10 ppm methyl methanesulfonate (positive control), and three concentrations of rhodamine B (200, 100, and 50 ppm) for 7 days. Rhodamine B inhibited mitotic activity; increased nuclear anomalies, including micronuclei, nuclear buds, and bridged nuclei; and induced oxidative stress in A. cepa root tissues. Furthermore, a substantial amount of long nucleoplasmic bridges were entangled together, and some nuclei were simultaneously linked to several other nuclei and to nuclear buds with nucleoplasmic bridges in rhodamine B-treated cells. In conclusion, rhodamine B induced cytogenetic effects in A. cepa root tip cells, which suggests that the A. cepa root is an ideal model system for detecting cellular interactions.
Dehong
D
Tan
Bing
B
Bai
Donghua
D
Jiang
Lin
L
Shi
Shunchang
S
Cheng
Dongbing
D
Tao
Shujuan
S
Ji
D060387:Ascorbate Peroxidases / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002467:Cell Nucleus / Q000187:drug effects / Q000473:pathology; D005456:Fluorescent Dyes / Q000633:toxicity*; D005979:Glutathione Peroxidase / Q000378:metabolism; D018519:Meristem / Q000166:cytology* / Q000187:drug effects*; D008938:Mitosis / Q000187:drug effects; D008940:Mitotic Index; D019697:Onions; D018384:Oxidative Stress; D012235:Rhodamines / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism
D005456:Fluorescent Dyes; D012235:Rhodamines; D060387:Ascorbate Peroxidases; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C029773:rhodamine B