Implantation phaeohyphomycosis caused by a non-sporulating Chaetomium species.
We report the case of a 66-year-old Iranian woman with a phaeohyphomycotic cyst (approximately 3×2.5cm in size) on the right lateral side of the neck. She had dysphagia and hoarseness, without any pain. She complained about discharge of black liquid on the skin and irritation. Histological examination of biopsy fragments from the lesions showed septate, branched brown hyphae. The fungus was cultured, but sporulation remained absent from 4- week-old cultures on Sabouraud dextrose agar (SDA), malt extract agar (MEA), potato dextrose agar (PDA), and water agar with sterile filter paper. Identification with the genus Chaetomium was achieved by sequencing the internal transcribed spacer (ITS) and the small subunit (SSU) domains of the rDNA gene and comparison with sequences held at GenBank and at the Centraalbureau voor Schimmelcultures (CBS). Sequencing of the SSU rRNA gene reveals this strain as belonging to the genus Chaetomium. The sequence of ITS did not fully match with any sequence of available ex-type strains of Chaetomium, Thielavia, Madurella and Papulaspora and hence might belong to an undescribed species. However, without diagnostic morphological features the taxon cannot be introduced as a novel member of the genus Chaetomium. After local excision of the cyst and antifungal therapy with ketoconazole (200mg twice a day), the lesion regressed and healed completely.
Department of Parasitology and Mycology, Cancer Molecular Pathology Research Center, Ghaem Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
M J
MJ
Najafzadeh
Department of Parasitology and Mycology, Research Center for Cutaneous Leishmaniasis, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: fataa@mums.ac.ir.
A
A
Fata
Department of Parasitology and Mycology, Imam Reza Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
A
A
Naseri
CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands; Food and Drug Deputy, Mashhad University of Medical Sciences, Mashhad, Iran.
M Saradeghi
MS
Keisari
Department of Medical Mycology and Parasitology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
S
S
Farahyar
Department of Parasitology and Mycology, Imam Reza Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
M
M
Ganjbakhsh
Hepatitis Research Center, Department of Internal Medicine, Faculty of medicine,Birjand University of Medical Sciences, Birjand, Iran.
M
M
Ziaee
CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands.
S
S
Dolatabadi
CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands; Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, The Netherlands; Peking University Health Science Center, Research Center for Medical Mycology, Beijing, PR China.
G S
GS
de Hoog
D000368:Aged; D002597:Chaetomium / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D004271:DNA, Fungal / Q000235:genetics; D005260:Female; D006801:Humans; D007121:Immunocompetence; D007492:Iran; D009333:Neck; D060446:Phaeohyphomycosis / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D017422:Sequence Analysis, DNA; D013172:Spores, Fungal
D004271:DNA, Fungal
Chaetomium; Cutaneous phaeohyphomycosis; Phaeohyphomycose cutanée; Sordariales