Protective effects of Notoginsenoside R1 on intestinal ischemia-reperfusion injury in rats.
Intestinal ischemia and reperfusion (I/R) is a clinical problem occurred for diverse causes with high mortality. Prophylaxis and treatment of intestinal I/R remains a challenge for clinicians. The purpose of the present study was to explore the role of Notoginsenoside R1 (R1), a major component form of Panax notoginseng, in management of intestinal I/R injury. Intestinal I/R was induced in male Sprague-Dawley rats by clamping the superior mesenteric artery for 90 min followed by reperfusion for 60 min or 3 days. R1 (10 mg·kg(-1)·h(-1)) was administered either 20 min before ischemia or 20 min after reperfusion. Intestinal microcirculation was evaluated by intravital microscopy over 60 min reperfusion. Sixty minutes or 3 days after reperfusion, rats were killed for histological examination of the jejunum tissue and immunohistochemical localization of myeloperoxidase and CD68. ATP, ADP, and AMP content in jejunum tissue was assessed by ELISA. Activation of nuclear factor-κB (NF-κB) and expression of ATP5D and tight junction proteins were determined by Western blotting. The results demonstrated that R1 is capable of attenuating intestinal I/R-induced microvascular hyperpermeability, inflammatory cytokine production, NF-κB activation, and loss of tight junction proteins, as well as improving energy metabolism during I/R. The results of the present study suggest R1 as an option in protecting against intestinal I/R injury.
Dept. of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking Univ., 38 Xueyuan Road, Beijing 100191, People's Republic of China. hanjingyan@bjmu.edu.cn.
Chong
C
Li
Quan
Q
Li
Yu-Ying
YY
Liu
Ming-Xia
MX
Wang
Chun-Shui
CS
Pan
Li
L
Yan
Yuan-Yuan
YY
Chen
Jing-Yu
JY
Fan
Jing-Yan
JY
Han
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002199:Capillary Permeability / Q000187:drug effects; D004734:Energy Metabolism / Q000187:drug effects; D036145:Ginsenosides / Q000494:pharmacology*; D007410:Intestinal Diseases / Q000209:etiology / Q000517:prevention & control*; D007422:Intestines / Q000098:blood supply; D007583:Jejunum / Q000166:cytology / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008833:Microcirculation / Q000187:drug effects; D020556:Neutrophil Infiltration / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow / Q000187:drug effects; D015427:Reperfusion Injury / Q000517:prevention & control*; D019108:Tight Junctions / Q000187:drug effects / Q000378:metabolism
D036145:Ginsenosides; D008315:Malondialdehyde; D009195:Peroxidase; C072936:notoginsenoside R1
adenosine 5′-triphosphate; inflammation; microcirculation; nuclear factor-κB; tight junction