A liposome-based assay for quantitation of detergents.
The amount of enzyme released from liposomes exposed to detergents varies with the amount of detergent present. This fact makes it possible to quantitate the detergent. Multilamellar liposomes containing entrapped peroxidase were adsorbed to paper discs and then exposed to solutions containing detergents. The assay procedure proved useful for assaying detergents down to their critical micelle concentration (CMC), and for an induced leakage of ionic detergents also below their CMCs.
Department of Pure and Applied Biochemistry, Chemical Center, University of Lund, PO Box 740, S-22007, Lund, Sweden.
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Eriksson
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Mattiasson