Comparison of microdialysis sampling perfusion fluid components on the foreign body reaction in rat subcutaneous tissue.
Microdialysis sampling is a commonly used technique for collecting solutes from the extracellular space of tissues in laboratory animals and humans. Large molecular weight solutes can be collected using high molecular weight cutoff (MWCO) membranes (100kDa or greater). High MWCO membranes require addition of high molecular weight dextrans or albumin to the perfusion fluid to prevent fluid loss via ultrafiltration. While these perfusion fluid additives are commonly used during microdialysis sampling, the tissue response to the loss of these compounds across the membrane is poorly understood. Tissue reactions to implanted microdialysis sampling probes containing different microdialysis perfusion fluids were compared over a 7-day time period in rats. The base perfusion fluid was Ringer's solution supplemented with either bovine serum albumin (BSA), rat serum albumin (RSA), Dextran-70, or Dextran-500. A significant inflammatory response to Dextran-70 was observed. No differences in the tissue response between BSA and RSA were observed. Among these agents, the BSA, RSA, and Dextran-500 produced a significantly reduced inflammatory response compared to the Dextran-70. This work demonstrates that use of Dextran-70 in microdialysis sampling perfusion fluids should be eliminated and replaced with Dextran-500 or other alternatives.
Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR 72701, USA; Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR 72701, USA.
Geoffrey D
GD
Keeler
Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR 72701, USA; Department of Biological Sciences, University of Arkansas, Fayetteville, AR 72701, USA.
Jeannine M
JM
Durdik
Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR 72701, USA; Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR 72701, USA. Electronic address: jstenken@uark.edu.
Julie A
JA
Stenken
D000818:Animals; D003911:Dextrans / Q000378:metabolism / Q000633:toxicity; D015314:Dialysis Solutions / Q000378:metabolism / Q000633:toxicity*; D004867:Equipment Design; D005549:Foreign-Body Reaction / Q000209:etiology* / Q000378:metabolism; D007552:Isotonic Solutions / Q000378:metabolism / Q000633:toxicity; D008297:Male; D017551:Microdialysis / Q000009:adverse effects* / Q000295:instrumentation / Q000379:methods; D008970:Molecular Weight; D017207:Rats, Sprague-Dawley; D000077331:Ringer's Solution; D012307:Risk Factors; D012710:Serum Albumin, Bovine / Q000378:metabolism / Q000633:toxicity; D040521:Subcutaneous Tissue / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors
D003911:Dextrans; D015314:Dialysis Solutions; D007552:Isotonic Solutions; D012710:Serum Albumin, Bovine; D000077331:Ringer's Solution
Biocompatibility; Dextran; Foreign body response; Rat; Subcutaneous tissue