Comet assay with gill cells of Mytilus galloprovincialis end point tools for biomonitoring of water antibiotic contamination: Biological treatment is a reliable process for detoxification.
This article investigates the ability of Pseudomonas peli to treat industrial pharmaceuticals wastewater (PW). Liquid chromatography-mass spectrometry (MS)/MS analysis revealed the presence, in this PW, of a variety of antibiotics such as sulfathiazole, sulfamoxole, norfloxacine, cloxacilline, doxycycline, and cefquinome.P. peli was very effective to be grown in PW and inducts a remarkable increase in chemical oxygen demand and biochemical oxygen demand (140.31 and 148.51%, respectively). On the other hand, genotoxicity of the studied effluent, before and after 24 h of shaking incubation with P. peli, was evaluated in vivo in the Mediterranean wild mussels Mytilus galloprovincialis using comet assay for quantification of DNA fragmentation. Results show that PW exhibited a statistically significant (p< 0.001) genotoxic effect in a dose-dependent manner; indeed, the percentage of genotoxicity was 122.6 and 49.5% after exposure to 0.66 ml/kg body weight (b.w.); 0.33 ml/kg b.w. of PW, respectively. However, genotoxicity decreased strongly when tested with the PW obtained after incubation with P. peli We can conclude that using comet assay genotoxicity end points are useful tools to biomonitor the physicochemical and biological quality of water. Also, it could be concluded that P. peli can treat and detoxify the studied PW.
Faculté de Médicine Dentaire, Laboratoire de Biologie Cellulaire et Moléculaire, Monastir, Tunisie.
Nadia
N
Mustapha
Laboratoire de Biotechnologie et Valorisation de Bio Géo Ressources (LBVBGR), Institut Supérieur de Biotechnologie (LR11-ES31), BioTechPole Sidi Thabet, Université de la Manouba, Sidi Thabet, Tunisie.
Amina
A
Zouiten
Laboratoire de Biotechnologie et Valorisation de Bio Géo Ressources (LBVBGR), Institut Supérieur de Biotechnologie (LR11-ES31), BioTechPole Sidi Thabet, Université de la Manouba, Sidi Thabet, Tunisie.
Dorra
D
Dridi
Laboratoire de Biotechnologie et Valorisation de Bio Géo Ressources (LBVBGR), Institut Supérieur de Biotechnologie (LR11-ES31), BioTechPole Sidi Thabet, Université de la Manouba, Sidi Thabet, Tunisie.
Leyla
L
Tahrani
Laboratoire d'Aquaculture, Institut Nationale des Sciences et Technologie de la Mer, Monastir, Tunisie.
Dorra
D
Zouiten
Laboratoire des Aliments Bioprocédés, Toxicologie, Environnements (ABTE-EA 4651), IUT de Caen, Université de Caen Basse-Normandie, Caen, France.
Ridha
R
Mosrati
Laboratoire de Biotechnologie et Valorisation de Bio Géo Ressources (LBVBGR), Institut Supérieur de Biotechnologie (LR11-ES31), BioTechPole Sidi Thabet, Université de la Manouba, Sidi Thabet, Tunisie.
Ameur
A
Cherif
Faculté de Médicine Dentaire, Laboratoire de Biologie Cellulaire et Moléculaire, Monastir, Tunisie.
Leila
L
Chekir-Ghedira
Laboratoire de Biotechnologie et Valorisation de Bio Géo Ressources (LBVBGR), Institut Supérieur de Biotechnologie (LR11-ES31), BioTechPole Sidi Thabet, Université de la Manouba, Sidi Thabet, Tunisie Laboratoire des Aliments Bioprocédés, Toxicologie, Environnements (ABTE-EA 4651), IUT de Caen, Université de Caen Basse-Normandie, Caen, France hdbenmansour@gmail.com.
Hedi Ben
HB
Mansour
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*; D002478:Cells, Cultured; D020552:Comet Assay; D053960:DNA Breaks / Q000187:drug effects*; D004784:Environmental Monitoring / Q000379:methods*; D005880:Gills / Q000166:cytology* / Q000378:metabolism; D008954:Models, Biological; D049878:Mytilus / Q000166:cytology* / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*
D000900:Anti-Bacterial Agents; D014874:Water Pollutants, Chemical
Mytilus galloprovincialis; Pharmaceutical wastewater; Pseudomonas peli; bio-monitoring; biodegradation; comet assay