Investigation of four candidate genes (IGF2, JHDM1A, COPB1 and TEF1) for growth rate and backfat thickness traits on SSC2q in Large White pigs.
As important quantitative traits, the growth rate and backfat thickness are controlled by multiple genes. The aim of this investigation was to evaluate the effect of the single and multiple SNPs of four candidate genes (IGF2, JHDM1A, COPB1 and TEF-1) on growth rate and backfat thickness. The four candidate genes were mapped on the p arm of SSC 2, and there are several QTLs, such as average daily gain, backfat thickness, an imprinted QTLs affecting muscle mass and fat deposition have been reported in this region. The polymorphisms of these genes were detected using PCR-RFLP methods, mixed procedure was used to analyze the single marker association with the growth and backfat thickness traits, and the gene-gene combination was investigated using multiple-markers analysis. The single marker association analysis indicated that the IGF2 intron-3 g.3072G > A and the substitution g.93G > A of TEF-1 gene were significantly associated with the age at 100 kg (P < 0.05). The JHDM1A 3′UTR g.224C > G, the c.3096C > T polymorphism of COPB1 gene and the substitution g.93G > A of TEF-1 gene were all significantly associated with the backfat at the shoulder (P < 0.05), backfat at the last rib, backfat at the lumbar, and the average backfat thickness, respectively. The multiple-markers analysis indicated that IGF2 and TEF-1 integrated gene networks for the age at 100 kg. Therefore, we can suggest that the polymorphism of IGF2 and TEF-1 gene could be used in marker-assisted selection for the age at 100 kg in Large White pigs.
Xuelei
X
Han
Huawei
H
Yang
Tengfei
T
Jiang
Qingde
Q
Zhang
Cuiping
C
Zeng
Bin
B
Fan
Bang
B
Liu
D050154:Adiposity / Q000235:genetics; D000818:Animals; D001415:Back / Q000254:growth & development; D001835:Body Weight; D033481:Chromosomes, Mammalian / Q000235:genetics; D020756:Coatomer Protein / Q000235:genetics*; D005787:Gene Frequency; D056726:Genetic Association Studies; D005819:Genetic Markers; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D050151:Subcutaneous Fat / Q000254:growth & development; D034421:Sus scrofa / Q000235:genetics* / Q000254:growth & development; D014157:Transcription Factors / Q000235:genetics*
D020756:Coatomer Protein; D005819:Genetic Markers; D014157:Transcription Factors; D007335:Insulin-Like Growth Factor II; D056484:Jumonji Domain-Containing Histone Demethylases