Spectroscopic studies on the interaction of Ga3+-hypocrellin A with myoglobin.
In this article, the interaction mechanism of Ga(3+)-hypocrellin A (Ga(3+)-HA) with myoglobin (Mb) is studied in detail through various spectroscopic technologies. UV-vis absorption and fluorescence spectra demonstrate the interaction process. The Stern-Volmer plot and the time-resolved fluorescence study suggest the fluorescence quenching mechanism of Mb by Ga(3+)-HA is a static quenching procedure, and the electronic transfer forces play a major role in binding Ga(3+)-HA to Mb. Furthermore, synchronous fluorescence studies and circular dichroism (CD) spectra reveal that the conformation of Mb is changed after its conjugation with Ga(3+)-HA.
College of Chemistry and Materials Science, Analysis and Testing Center, Jiangsu Key Laboratory Biofunctional Materials, Key Laboratory of Applied Photochemistry, JiangSu Collaborative Innovation Center of Biomedical Functional Materials, Nanjing Normal University, Wenyuan Road No. 1, Nanjing 210023, PR China.
Wenli
W
Xie
College of Chemistry and Materials Science, Analysis and Testing Center, Jiangsu Key Laboratory Biofunctional Materials, Key Laboratory of Applied Photochemistry, JiangSu Collaborative Innovation Center of Biomedical Functional Materials, Nanjing Normal University, Wenyuan Road No. 1, Nanjing 210023, PR China.
Shaohua
S
Wei
State Key Laboratory of Natural Medicines, Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing 210038, PR China.
Jihua
J
Liu
College of Chemistry and Materials Science, Analysis and Testing Center, Jiangsu Key Laboratory Biofunctional Materials, Key Laboratory of Applied Photochemistry, JiangSu Collaborative Innovation Center of Biomedical Functional Materials, Nanjing Normal University, Wenyuan Road No. 1, Nanjing 210023, PR China.
Xuefeng
X
Ge
College of Chemistry and Materials Science, Analysis and Testing Center, Jiangsu Key Laboratory Biofunctional Materials, Key Laboratory of Applied Photochemistry, JiangSu Collaborative Innovation Center of Biomedical Functional Materials, Nanjing Normal University, Wenyuan Road No. 1, Nanjing 210023, PR China. Electronic address: zhoulin@njnu.edu.cn.
Lin
L
Zhou
College of Chemistry and Materials Science, Analysis and Testing Center, Jiangsu Key Laboratory Biofunctional Materials, Key Laboratory of Applied Photochemistry, JiangSu Collaborative Innovation Center of Biomedical Functional Materials, Nanjing Normal University, Wenyuan Road No. 1, Nanjing 210023, PR China. Electronic address: zhoujiahong@njnu.edu.cn.
Jiahong
J
Zhou
College of Chemistry and Materials Science, Analysis and Testing Center, Jiangsu Key Laboratory Biofunctional Materials, Key Laboratory of Applied Photochemistry, JiangSu Collaborative Innovation Center of Biomedical Functional Materials, Nanjing Normal University, Wenyuan Road No. 1, Nanjing 210023, PR China.
Jian
J
Shen
D000042:Absorption; D002942:Circular Dichroism; D004735:Energy Transfer; D005708:Gallium / Q000737:chemistry*; D007700:Kinetics; D009211:Myoglobin / Q000737:chemistry*; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry; D019800:Phenol; D017433:Protein Structure, Secondary; D011809:Quinones / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013997:Time Factors
D009211:Myoglobin; D011809:Quinones; D019800:Phenol; D010569:Perylene; D005708:Gallium; C048388:hypocrellin A
Circular dichroism spectroscopy; Ga(3+)-hypocrellin A; Myoglobin; Steady state fluorescence spectroscopy; Time-resolved fluorescence spectroscopy; UV–vis absorption spectroscopy