Effect of mitochondria-targeted antioxidant SkQ1 on programmed cell death induced by viral proteins in tobacco plants.
Programmed cell death (PCD) is the main defense mechanism in plants to fight various pathogens including viruses. The best-studied example of virus-induced PCD in plants is Tobacco mosaic virus (TMV)-elicited hypersensitive response in tobacco plants containing the N resistance gene. It was previously reported that the animal mitochondrial protein Bcl-xL, which lacks a homolog in plants, effectively suppresses plant PCD induced by TMV p50 - the elicitor of hypersensitive response in Nicotiana tabacum carrying the N gene. Our studies show that the mitochondria-targeted antioxidant SkQ1 effectively suppresses p50-induced PCD in tobacco plants. On the other hand, SkQ1 did not affect Poa semilatent virus TGB3-induced endoplasmic reticulum stress, which is followed by PCD, in Nicotiana benthamiana epidermal cells. These data suggest that mitochondria-targeted antioxidant SkQ1 can be used to study molecular mechanisms of PCD suppression in plants.
Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia.
A D
AD
Solovieva
O Yu
OY
Frolova
A G
AG
Solovyev
S Yu
SY
Morozov
A A
AA
Zamyatnin
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects / Q000502:physiology; D006801:Humans; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D010942:Plant Viruses; D010971:Plastoquinone / Q000031:analogs & derivatives* / Q000494:pharmacology; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
C532038:10-(6'-plastoquinonyl)decyltriphenylphosphonium; D000975:Antioxidants; D014764:Viral Proteins; D010971:Plastoquinone