Long-term treatment of hydrogen-rich saline abates testicular oxidative stress induced by nicotine in mice.
OBJECTIVE
The present study was designed to test the hypothesis that long-term treatment with hydrogen-rich saline abated testicular oxidative stress induced by nicotine in mice.


METHODS
The effects of hydrogen-rich saline (6 ml/kg, i.p.), vitamin C (60 mg/kg, i.p.) and vitamin E (100 mg/kg, i.p.) on reproductive system and testicular oxidative levels in nicotine-treated (4.5 mg/kg, s.b.) mice were investigated.


RESULTS
It was found that vitamin C and vitamin E attenuated serum oxidative level, but did not lower testicular oxidative levels in mice subjected to chronic nicotine treatment, and did not improve the male reproductive damage and apoptosis induced by nicotine. Different from normal antioxidants, vitamin C and vitamin E, hydrogen-rich saline abated oxidative stress in testis, and protected against nicotine-induced male reproductive damages.


CONCLUSIONS
Our results first demonstrated that long-term treatment with hydrogen-rich saline attenuated testicular oxidative level and improved male reproductive function in nicotine-treated mice.
Department of Pharmacy, Navy General Hospital, No. 6 Fucheng Road, Beijing, 100048, People's Republic of China.
Shu
S
Li
DanDan
D
Lu
Yaling
Y
Zhang
Yi
Y
Zhang
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D019610:Cytoprotection / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D006859:Hydrogen / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009538:Nicotine / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D012965:Sodium Chloride / Q000737:chemistry / Q000494:pharmacology*; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors
D000975:Antioxidants; D012965:Sodium Chloride; D009538:Nicotine; D006859:Hydrogen