Regeneration of plants from achenes and petals of Chrysanthemum coccineum.
Achenes and petals of Chrysanthemum coccineum were cultured on MS and White medium supplemented with BA and NAA or 2,4-D. Without being transferred, shoots were formed directly and immediately after callus formation on the surface of the achene walls and from the cut ends of the petals. High concentracions of BA and NAA supported the callus induction and shoot formation, but higher concentrations of 2,4-D inhibited shoot formation. The shoots obtained from both explants formed roots when transferred to hormone-free medium and they could be transplanted to soil for further growth. The regenerated plants contained as much pyrethrins as the original plants.
Takasaki Research Laboratories, Nippon Kayaku Co., 370-12, Takasaki, Japan.
Y
Y
Fujii
K
K
Shimizu