[The effect of vascular endothelial cells on the proliferation of periodontal ligament cells and gingival fibroblasts].
OBJECTIVE
To investigate the effect of vascular endothelial cells on the proliferation of periodontal ligament cells and gingival fibroblasts through a model mimicking the periodontal wound healing and regeneration.


METHODS
Human vascular endothelial cells, periodontal ligament cells and gingival fibroblasts were co-cultured using a Transwell system. The number of each type of cells at 2, 4, 6, 8 and 10 days after co-culture were counted using a cell counter machine, while periodontal ligament cells and gingival fibroblasts single culture were used as control. The results were statistically processed with SPSS 13.0 software package.


RESULTS
When vascular endothelial cell was present, the proliferation of periodontal ligament cells and gingival fibroblasts were faster than each single culture, the difference between them was significant (P<0.01). The proliferation of periodontal ligament cells was faster than gingival fibroblast as they were co-cultured with vascular endothelial cells(which happened at day 6), although the results were contrary when they were single cultured, the difference between them was significant (P<0.01).


CONCLUSIONS
The presence of vascular endothelial cells can promote the proliferation of periodontal cells, and the effect is more prominent on human periodontal ligament cells than on gingival fibroblasts.
Department of Periodontics, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China.E-mail:cqxiaoding2009@126.com.
Jin
J
Din
Yuan-zheng
YZ
Yin
D049109:Cell Proliferation; D002478:Cells, Cultured; D042783:Endothelial Cells; D005347:Fibroblasts; D005881:Gingiva; D006801:Humans; D010513:Periodontal Ligament; D012038:Regeneration; D014945:Wound Healing