Identification and expression analysis of zebrafish glypicans during embryonic development.
Heparan sulfate Proteoglycans (HSPG) are ubiquitous molecules with indispensable functions in various biological processes. Glypicans are a family of HSPG's, characterized by a Gpi-anchor which directs them to the cell surface and/or extracellular matrix where they regulate growth factor signaling during development and disease. We report the identification and expression pattern of glypican genes from zebrafish. The zebrafish genome contains 10 glypican homologs, as opposed to six in mammals, which are highly conserved and are phylogenetically related to the mammalian genes. Some of the fish glypicans like Gpc1a, Gpc3, Gpc4, Gpc6a and Gpc6b show conserved synteny with their mammalian cognate genes. Many glypicans are expressed during the gastrulation stage, but their expression becomes more tissue specific and defined during somitogenesis stages, particularly in the developing central nervous system. Existence of multiple glypican orthologs in fish with diverse expression pattern suggests highly specialized and/or redundant function of these genes during embryonic development.
DFG- Center for Regenerative Therapies Dresden/ Cluster of Excellence (CRTD), Biotechnology Center, Technische Universität Dresden, Dresden, Saxony, Germany.
Mansi
M
Gupta
Michael
M
Brand
D000818:Animals; D002490:Central Nervous System / Q000196:embryology / Q000378:metabolism; D053673:Glypicans / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism*
D053673:Glypicans