Comparison of 3,5,3'-triiodo-L-thyronine and L-thyroxine absorption from the intestinal lumen of the fasted rainbow trout, Oncorhynchus mykiss.
The absorptions of 3,5,3'-triiodo-L-thyronine (T3) and L-thyroxine (T4) from the intestinal lumen of the rainbow trout were compared in vivo. Tracer doses of [(125)I]T4 ((+)T4) or [(125)I]T3 ((*)T3) were injected through an anal cannula into the duodenum of trout fasted for 3 days at 12°C, and radioactivity was measured in blood and tissues at 4-48 h. (*)T3 was removed more extensively than (*)T4 from the intestinal lumen and more radioactivity was absorbed into the blood and tissues of u+T3-injected trout than (*)T4-injected trout. HPLC analysis showed that a high proportion of the radioactivity in the plasma, liver, kidney and intestinal lumen of (*)T3-injected trout remained as the parent (*)T3. However, in (*)T4-injected trout most plasma radioactivity was in the form of (125)I(-), and by 24 h a high proportion of luminal radioactivity was (125)I(-). By 48 h, over 4% of the injected (*)T3 and 1% of the injected (*)T4 dose resided in the gall bladder, primarily as derivatives of (*)T3 or (*)T4. We conclude that T3 is absorbed more effectively than T4 from the intestinal lumen of fasted trout, indicating the potential for an enterohepatic T3 cycle.
Department of Zoology, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
A
A
Whitaker
J G
JG
Eales