[Extraction and purification of isocorydine from Dicranostigma leptopodum].
OBJECTIVE
To optimize the conditions of extraction and purification of isocorydine from Dicranostigma leptopodum.


METHODS
Extraction conditions of isocorydine were selected on the basis of orthogonal experimental design, the static adsorption/desorption experiments were used to evaluate the optimum resin.


RESULTS
The optimum extraction parameters were as follows: the ratio of raw materials to solvent 1:15 (g/mL), extraction solvent 1% vitriol, the extraction three times and 1 h each time. LX28 resin exhibited higher adsorption efficiency.


CONCLUSIONS
Under the above optimum conditions, the extraction yield of isocorydine is 0.88%. The purity of isocorydine can reach 85.34% with a yield rate of 68.64%.
College of Life Science & Engineering, Lanzhou University of Technology, Lanzhou 730050, China.
Yan
Y
Feng
Ai-Mei
AM
Yang
Jun-Xi
JX
Liu
Duo-Long
DL
Di
Yan-Juan
YJ
Liu
Min
M
Li
D000327:Adsorption; D001060:Aporphines / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D000432:Methanol / Q000737:chemistry; D028101:Papaveraceae / Q000737:chemistry*; D016062:Porosity; D012117:Resins, Synthetic / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*; D014465:Ultrasonics
D001060:Aporphines; D004365:Drugs, Chinese Herbal; D012117:Resins, Synthetic; C030168:isocorydine; D000432:Methanol