Triplet-state monitoring by fluorescence correlation spectroscopy.
The effects of high excitation intensities in fluorescence correlation spectroscopy (FCS) in terms of saturation and triplet-state build-up have been studied for the case of Rh6G in aqueous solution. It was found that FCS provides a powerful means for the determination of intersystem crossing and triplet-state depopulation rates of fluorophores in solution.
Department of Medical Biochemistry and Biophysics, Karolinska Institute, S-171 77, Stockholm, Sweden.
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Widengren
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Rigler
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Mets