The effects of rejuvenation during hypothermic storage on red blood cell membrane remodeling.
BACKGROUND
Our previous studies showed that hypothermic storage (HS) induces red blood cell (RBC) microparticle (RMP) generation and changes in phosphatidylserine (PS) and CD47 expression on RBCs and RMPs. The aim of this study was to evaluate the effect of cold rejuvenation treatment at multiple time points during storage on these prehemolytic indicators of RBC membrane storage lesion.


METHODS
Leukoreduced RBC units in saline-adenine-glucose-mannitol were used to generate three groups: untreated controls, sham-treated units, and units treated with a cold (1-6°C) rejuvenation solution on Day 28, 35, or 42 of HS. Units were assessed for hemolysis, adenosine triphosphate (ATP) concentration, lipid composition, and RMP generation, as well as PS and CD47 expression throughout 49 days of HS.


RESULTS
Rejuvenation treatment led to a significant increase in ATP concentration in all units, irrespective of treatment day. There were no significant differences between sham- and rejuvenation-treated RBC samples in the levels of PS externalization, CD47 expression, or the rate of RMP formation. RBCs rejuvenated on Day 28 were enriched in glycerophosphocholine (+23.5%), depleted in sphingomyelin (-14%), and slightly depleted in cholesterol (-3.5%).


CONCLUSIONS
Cold rejuvenation in hypothermically stored RBCs affects the lipid composition of RBCs and respective RMPs in a time-dependent fashion.
Research and Development, Canadian Blood Services, Edmonton, Alberta, Canada.
Jayme D R
JD
Kurach
Ruqayyah
R
Almizraq
Beatriz
B
Bicalho
Jason P
JP
Acker
Jelena L
JL
Holovati
D001793:Blood Preservation / Q000379:methods*; D002462:Cell Membrane / Q000378:metabolism*; D003080:Cold Temperature; D004912:Erythrocytes / Q000166:cytology* / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D047589:Leukocyte Reduction Procedures