Targeting ribonucleic acids by toxic small molecules: structural perturbation and energetics of interaction of phenothiazinium dyes thionine and toluidine blue O to tRNA phe.
This study was designed to examine the toxic interaction of two phenothiazinium dyes thionine (TO) and toluidine blue O (TBO) with tRNA(phe) by spectroscopic and calorimetric techniques. While phenothiazinium dye complexation with DNA is known, their bindings to RNA are not fully investigated. The non cooperative binding of both the dyes to tRNA was revealed from absorbance and fluorescence studies. From absorption, steady-state emission, the effect of ferrocyanide ion-induced steady-state fluorescence quenching, circular dichroism, the mode of binding of these dyes into the tRNA helix has been substantiated to be principally by intercalative in nature. Both dyes enhanced the thermal stability of tRNA. Circular dichroism studies provided evidence for the structural perturbations associated with the tRNA structure with induction of optical activity in the CD inactive dye molecules. Results from isothermal titration calorimetry experiments suggested that the binding of both dyes was predominantly entropy driven with a smaller but favorable enthalpy term that increased with temperature. The binding was dependent on the Na(+) concentration, but had a larger non-electrostatic contribution to the Gibbs energy. A small heat capacity value and the enthalpy-entropy compensation in the energetics of the interaction characterized the binding of the dyes to tRNA. This study confirms that the tRNA(phe) binding affinity is greater for TO compared to TBO. The utility of the present work lies in understanding the potential binding and consequent damage to tRNA by these toxic dyes in their development as therapeutic agents.
Biophysical Chemistry Laboratory, Chemistry Division, CSIR-Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Road, Kolkata 700032, India.
Puja
P
Paul
Gopinatha Suresh
GS
Kumar
D000042:Absorption; D001483:Base Sequence; D002151:Calorimetry; D002942:Circular Dichroism; D004396:Coloring Agents / Q000032:analysis / Q000737:chemistry*; D019277:Entropy; D005456:Fluorescent Dyes / Q000737:chemistry; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010640:Phenothiazines / Q000737:chemistry*; D010649:Phenylalanine / Q000737:chemistry*; D012331:RNA, Fungal / Q000737:chemistry*; D012343:RNA, Transfer / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D014048:Tolonium Chloride / Q000737:chemistry*; D014783:Viscosity
D004396:Coloring Agents; D005456:Fluorescent Dyes; D010640:Phenothiazines; D012331:RNA, Fungal; D012996:Solutions; D014048:Tolonium Chloride; D010649:Phenylalanine; D012343:RNA, Transfer; C009469:thionine
Calorimetry; Intercalation; Phenothiazinium dyes; Spectroscopy