Differential aspects of immune cell infiltration and neurodegeneration in acute and relapse experimental autoimmune encephalomyelitis.
Proteolipid protein (PLP)-induced experimental autoimmune encephalomyelitis (EAE) in SJL/J mice is a well-established animal model for the study of relapsing-remitting multiple sclerosis (MS). Here we investigated histomorphological differences between acute and relapse EAE in order to get further insights into the mechanisms that trigger the transition from a relapsing-remitting course to chronic-progressive disease. We found the extent of inflammation to differ in respect to the type of immune cells infiltrating the CNS and the manifestation of edema. Myelin pathology was predominated by demyelinated axons in the acute phase. In relapse EAE the extent of myelin pathology declined and was characterized by a balance between demyelinated and demyelinating nerve fibers. Axonal pathology increased with disease progression and was partly separated from myelin pathology and inflammation. The reported incisive differences between acute and relapse EAE suggest a transition from inflammatory processes to independent neurodegeneration in the disease course.
Department of Anatomy I, Joseph-Stelzmann-Str. 9, University of Cologne, 50931 Cologne, Germany.
Isabel A
IA
Soellner
Jennifer
J
Rabe
Victor
V
Mauri
Janina
J
Kaufmann
Klaus
K
Addicks
Stefanie
S
Kuerten
D000208:Acute Disease; D000818:Animals; D001369:Axons / Q000276:immunology / Q000473:pathology; D002465:Cell Movement; D018450:Disease Progression; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000276:immunology / Q000473:pathology*; D005260:Female; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology / Q000473:pathology; D007962:Leukocytes / Q000276:immunology / Q000473:pathology*; D051379:Mice; D018991:Myelin Proteolipid Protein; D009410:Nerve Degeneration / Q000139:chemically induced / Q000276:immunology / Q000473:pathology; D009412:Nerve Fibers / Q000276:immunology / Q000473:pathology; D010446:Peptide Fragments; D012008:Recurrence; D013116:Spinal Cord / Q000276:immunology / Q000473:pathology
D018991:Myelin Proteolipid Protein; D010446:Peptide Fragments; C435023:myelin proteolipid protein (139-151)
BSA; CFA; CNS; EAE; Electron microscopy; GA; HE; IFA; IHC; Inflammation; MOG; MS; NF; NNND; Neurodegeneration; PFA; PLP; PLP:139–151; PTx; ROS; bovine serum albumin; central nervous system; complete Freund's adjuvant; experimental autoimmune encephalomyelitis; glutaraldehyde; haematoxylin/eosin; immunohistochemistry; incomplete Freund's adjuvant; multiple sclerosis; myelin oligodendrocyte glycoprotein; nearest neighbor neurofilament distance; neurofilaments; paraformaldehyde; pertussis toxin; proteolipid protein; reactive oxygen species