In-vitro auxin transport in membrane vesicles from maize coleoptiles.
When membrane vesicles from maize (Zea mays L.) coleoptiles are extracted at high buffer strength, a pH-driven, saturable association of [(14)C] indole-3-acetic acid is found, similar to the in-vitro auxin-transport system previously described for Cucurbita hypocotyls. The phytotropins naphthylphthalamic acid and pyrenoylbenzoic acid increase net uptake, pressumably by inhibiting the auxin-efflux carrier.
Institut für Biologie III der Universität, Schänzlestrasse 1, D-7800, Freiburg i. Br., Federal Republic of Germany.
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Heyn
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Hoffmann
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Hertel