[Microbiological profile of acute viral gastroenteritis attended in a paediatric department of an area with high vaccine cover against rotavirus].
BACKGROUND
Vaccination against rotavirus has led to a significant decline of the disease. The aim of the present work is to evaluate the clinical and epidemiological features of the viral acute gastroenteritis (AGE) in an area with high immunization coverage against rotavirus.


METHODS
A prospective microbiological evaluation was made of stool culture and Real Time Polymerase Chain Reaction (RT-PCR) to gastroenteric virus and genotyping of rotavirus strains in < 5 year-old with AGE episodes attended by or admitted to our hospital from November-March of 2009-2010 and 2010-2011.


RESULTS
A total of 51 patients were included, with a mean age (standard deviation) of 19.1 (13.9) months. Stool culture was negative in 23 samples (45% of the samples analyzed), and it was identified a responsible microorganism in 70% by the RT-PCR (16 samples). Rotavirus was the most common isolated microorganism (53%), and G1[P8] the most frequent genotype. A co-infection was detected in 14% of samples (7 patients), and rotavirus and astrovirus were the most frequent etiological agents involved.


CONCLUSIONS
Rotavirus, basically G1[P8], is the most common AGE responsible agent identified in our study. The use of RT-PCR enhances the AGE diagnostic sensitivity, and uncovers an important number of viral co-infections.
Área Integrada de Pediatría, Complejo Hospitalario Universitario de Santiago de Compostela, Santiago de Compostela, España.
Carlos
C
García-Magán
Área Integrada de Pediatría, Complejo Hospitalario Universitario de Santiago de Compostela, Santiago de Compostela, España.
María José
MJ
de Castro-López
Servicio de Microbiología, Complejo Hospitalario Universitario de Santiago de Compostela, Santiago de Compostela, España.
José
J
Llovo-Taboada
Área Integrada de Pediatría, Complejo Hospitalario Universitario de Santiago de Compostela, Santiago de Compostela, España.
Carmen
C
Curros-Novo
Área Integrada de Pediatría, Complejo Hospitalario Universitario de Santiago de Compostela, Santiago de Compostela, España.
Miriam
M
Puente-Puig
Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, España.
Alicia
A
Sánchez-Fauquier
Área Integrada de Pediatría, Complejo Hospitalario Universitario de Santiago de Compostela, Santiago de Compostela, España. Electronic address: federico.martinon.torres@sergas.es.
Federico
F
Martinón-Torres
D000208:Acute Disease; D005260:Female; D005759:Gastroenteritis / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D006748:Hospital Departments; D006801:Humans; D007223:Infant; D008297:Male; D010372:Pediatrics; D011446:Prospective Studies; D012400:Rotavirus Infections / Q000517:prevention & control*; D022243:Rotavirus Vaccines; D014611:Vaccination / Q000706:statistics & numerical data
D022243:Rotavirus Vaccines
Cobertura vacunal; Coinfección; Coinfection; Gastroenteritis; Genotipificado; Genotyping; Immunization coverage; Rotavirus; Surveillance; Vigilancia epidemiológica