Phage as a template to grow bone mineral nanocrystals.
Phage display is a biotechnique that fuses functional peptides on the outer surface of filamentous phage by inserting DNA encoding the peptides into the genes of its coat proteins. The resultant peptide-displayed phage particles have been widely used as biotemplates for the synthesis of functional hybrid nanomaterials. Here, we describe the bioengineering of M13 filamentous phage to surface-display bone mineral (hydroxyapatite (HAP))-nucleating peptides derived from dentin matrix protein-1 and using the engineered phage as a biotemplate to grow HAP nanocrystals.
Department of Chemistry & Biochemistry, Stephenson Life Sciences Center, University of Oklahoma, Norman, OK, USA.
Binrui
B
Cao
Hong
H
Xu
Chuanbin
C
Mao
D017104:Bacteriophage M13 / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D001842:Bone and Bones / Q000378:metabolism*; D061505:Cell Surface Display Techniques; D017886:Durapatite / Q000737:chemistry / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism*; D010012:Osteogenesis; D019151:Peptide Library; D010455:Peptides / Q000235:genetics / Q000378:metabolism
D016326:Extracellular Matrix Proteins; D019151:Peptide Library; D010455:Peptides; D017886:Durapatite