Somatic embryogenesis and plant regeneration from immature zygotic embryos of papaya (Carica papaya L.).
Immature zygotic embryos from open-pollinated and selfed Carica papaya L. fruits, 90 to 114 days post-anthesis, produced 2 to 20 somatic embryos on apical domes, cotyledonary nodes, and radicle meristems after culture for three weeks on half-strength Murashige and Skoog (MS) medium supplemented with 0.1 to 25 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D), 400 mg l(-1) glutamine, and 6% sucrose. After six weeks of culture, about 40 to 50% of the zygotic embryos had become embryogenic, and each embryogenic embryo yielded hundreds of somatic embryos within five months of culture on media supplemented with 2,4-D. Somatic embryos matured on half-strength MS medium, germinated on MS medium containing 5 mg l(-1) kinetin, and grew large enough for greenhouse culture on MS medium. Shoots were rooted in vermiculite and grown in the greenhouse.
Department of Horticulture, University of Hawaii, 3190 Maile Way, 96822, Honolulu, HI, USA.
M M
MM
Fitch
R M
RM
Manshardt