Dengue immunoassay with an LSPR fiber optic sensor.
Dengue fever is a viral disease that affects millions of people worldwide. Specific tests for dengue are not usually performed due to high costs, complicated procedures and, in some cases, long time to yield a result. For widespread use of specific tests to be possible, fast, reliable and fairly simple methods are needed. In this paper, we present a new dengue diagnostic method for the acute phase of the infection. The method proposed uses an all-optical fiber sensor based on Localized Surface Plasmon Resonance (LSPR) and specular reflection from gold nanoparticles (AuNPs). Dengue anti-NS1 antibody was immobilized on AuNPs deposited on the endface of a standard multimode fiber (62.5 µm/125 µm). The sensor is able to detect NS1 antigen at different concentrations, with limit of quantification estimated to be 0.074 μg/ml = 1.54 nM. These results indicate that the sensor could potentially be used for dengue diagnosis in the acute phase of the infection.
Alexandre R
AR
Camara
Paula M P
PM
Gouvêa
Ana Carolina M S
AC
Dias
Arthur M B
AM
Braga
Rosa F
RF
Dutra
Renato E
RE
de Araujo
Isabel C S
IC
Carvalho
D015374:Biosensing Techniques / Q000295:instrumentation*; D003716:Dengue Virus / Q000276:immunology / Q000302:isolation & purification*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D007118:Immunoassay / Q000295:instrumentation*; D012031:Refractometry / Q000295:instrumentation*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D017361:Viral Nonstructural Proteins / Q000032:analysis / Q000276:immunology
C082515:NS1 protein, dengue-1 virus; D017361:Viral Nonstructural Proteins