A protocol for high-throughput Agrobacterium-mediated barley transformation.
Agrobacterium-mediated transformation of barley is a valuable tool for determining gene function by over-expression of a gene of interest or by RNAi-based gene silencing. The method is based on the inoculation of immature embryos with Agrobacterium and uses a hygromycin resistance gene to allow selection of transgenic plants. The protocol described leads to average transformation efficiencies of 25 % meaning that large numbers of fertile transgenic plants can be produced.
Department of Crop Genetics, John Innes Centre, Norwich, UK.
Wendy A
WA
Harwood
D060054:Agrobacterium / Q000235:genetics* / Q000378:metabolism; D018014:Gene Transfer Techniques; D001467:Hordeum / Q000235:genetics* / Q000382:microbiology; D030821:Plants, Genetically Modified; D012639:Seeds / Q000235:genetics / Q000382:microbiology; D014170:Transformation, Genetic