Physiological and proteomic changes suggest an important role of cell walls in the high tolerance to metals of Elodea nuttallii.
Macrophytes bioaccumulate metals, the suggestion being made that they be considered for phytoremediation. However, a thorough understanding of the mechanisms of metal tolerance in these plants is necessary to allow full optimization of this approach. The present study was undertaken to gain insight into Hg and Cd accumulation and their effects in a representative macrophyte, Elodea nuttallii. Exposure to methyl-Hg (23 ng dm(-3)) had no significant effect while inorganic Hg (70 ng dm(-3)) and Cd (281 μg dm(-3)) affected root growth but did not affect shoots growth, photosynthesis, or antioxidant enzymes. Phytochelatins were confirmed as having a role in Cd tolerance in this plant while Hg tolerance seems to rely on different mechanisms. Histology and subcellular distribution revealed a localized increase in lignification, and an increased proportion of metal accumulation in cell wall over time. Proteomics further suggested that E. nuttallii was able to efficiently adapt its energy sources and the structure of its cells during Hg and Cd exposure. Storage in cell walls to protect cellular machinery is certainly predominant at environmental concentrations of metals in this plant resulting in a high tolerance highlighted by the absence of toxicity symptoms in shoots despite the significant accumulation of metals.
Institut F.-A. Forel, University of Geneva, 10, route de Suisse, CP 416, CH-1290 Versoix, Switzerland.
Floriane
F
Larras
Nicole
N
Regier
Sébastien
S
Planchon
John
J
Poté
Jenny
J
Renaut
Claudia
C
Cosio
D000975:Antioxidants / Q000737:chemistry; D001673:Biodegradation, Environmental; D019328:Buthionine Sulfoximine / Q000737:chemistry; D002104:Cadmium / Q000737:chemistry; D019256:Cadmium Chloride / Q000737:chemistry; D002473:Cell Wall / Q000187:drug effects*; D015180:Electrophoresis, Gel, Two-Dimensional; D005782:Gels / Q000737:chemistry; D031321:Hydrocharitaceae / Q000187:drug effects*; D007700:Kinetics; D008031:Lignin / Q000737:chemistry; D008627:Mercuric Chloride / Q000737:chemistry; D008628:Mercury / Q000737:chemistry; D008670:Metals / Q000737:chemistry*; D009195:Peroxidase / Q000737:chemistry; D010788:Photosynthesis / Q000187:drug effects; D054811:Phytochelatins / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry; D018517:Plant Roots / Q000187:drug effects; D018520:Plant Shoots / Q000187:drug effects; D040901:Proteomics / Q000379:methods*; D012989:Soil Pollutants / Q000032:analysis; D013482:Superoxide Dismutase / Q000737:chemistry
D000975:Antioxidants; D005782:Gels; D008670:Metals; D010940:Plant Proteins; D012989:Soil Pollutants; D002104:Cadmium; D019328:Buthionine Sulfoximine; D008627:Mercuric Chloride; D008031:Lignin; D054811:Phytochelatins; D009195:Peroxidase; D013482:Superoxide Dismutase; D008628:Mercury; D019256:Cadmium Chloride
Cadmium; Inorganic mercury; Lignin; Methyl-mercury; Toxicokinetics