[Traffic-related PM2.5 regulates IL-2 releasing in Jurkat T cells by calcium signaling pathway].
OBJECTIVE
To explore the effects of traffic-related PM2.5 on interleukin-2 (IL-2) in Jurkat T cells and the regulatory action of calcium signaling pathway.


METHODS
The cells were exposed to 100 microg/ml of PM2.5 for 3, 6 and 24 h. Normal saline group, blank filter group, calcium chelating agent EGTA group and the calcineurin antagonist cyclosporine A (CSA) group were as parallel control. The level of IL-2 was detected by ELISA kits, the mRNA expression of CaN, NFAT were determined by QRT-PCR. The nuclear distribution of NFAT was observed by immunofluorescence microscopy.


RESULTS
The level of IL-2 in Jurkat T cells exposed to 100 microg/ml PM2.5 was significantly lower than parallel groups, but higher than PM2.5 + CSA group and PM2.5 + EGTA group (P < 0.05). With the increase of time, the releasing level of IL-2 appeared reducing trend in 100 microg/ml of PM2.5 group. The mRNA expression level of NFAT and CaN were higher than parallel groups, PM2.5 + CSA group and PM2.5 + EGTA group (P < 0.05). PM2.5 can induce NFAT protein with dephosphorylation and be activated, and NFAT protein can shift into nuclear. The level of IL-2 was negatively associated with the expression level of NFAT and CaN gene (P < 0.05).


CONCLUSIONS
Traffic-related PM2.5 may inhibit the releasing of IL-2, Ca(2+)-CaN-NFAT signal pathway may involve in the regulation of IL-2.
Department of Environment Health, School of Public Health, Shanxi Medical University, Taiyuan 030001, China. Tonggq1987@163.com
Guoqiang
G
Tong
Zhihong
Z
Zhang
Jianbiao
J
Han
Yong
Y
Qiu
Jianjun
J
Xu
D000393:Air Pollutants / Q000633:toxicity*; D020013:Calcium Signaling / Q000502:physiology*; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism*; D019169:Jurkat Cells; D050778:NFATC Transcription Factors / Q000235:genetics / Q000378:metabolism; D052638:Particulate Matter / Q000633:toxicity*; D001335:Vehicle Emissions
D000393:Air Pollutants; D007376:Interleukin-2; D050778:NFATC Transcription Factors; D052638:Particulate Matter; D001335:Vehicle Emissions