[Study on the diterpenoids of Tripterygium wilfordii by electrospray ionization tandem and electron impact mass spectrometry].
OBJECTIVE
To study the diterpenoids of Tripterygium wilfordii by electrospray ionization tandem (ESI) and electron impact (EI) mass spectrometry and establish the methods for quickly and on line identification of these diterpenoids.


METHODS
The diterpenoids were analyzed by ESI-MS and EI-MS under positive ion model.


RESULTS
In ESI-Trap-MS (+) model, the quasi-molecular ions [M + H] + of the diterpenoids took place bond cleavage and generally lost neutral molecules such as H2O, CH2CHCH3, CO and CH2CO, then generated characteristic fragment ion series (m/z 197, 183, 169). In ESI-TOF-MS (+) model, the quasi-molecular ions [M + H] + of the diterpenoids took place bond cleavage and generally lost neutral molecules such as H2O, CO, then generated characteristic fragment ion series (m/z 277, 185, 93). In EI-MS model, the molecular ions of the diterpenoids took place bond cleavage and generally lost neutral molecules such as H2O, CO, CH4, and isopropyl radical, then generated characteristic fragment ion series (m/z 149, 105, 91, 71, 55, 43).


CONCLUSIONS
The ESI-MS and EI-MS characteristics of diterpenoids of Tripterygium wilfordii are reported for the first time. Based on these characteristics, the methods for quickly and on line identification of diterpenoids are established.
Key Laboratory of Modem Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China. liu5308@sina.com
Jian-Qun
JQ
Liu
Ji-Cheng
JC
Shu
Rui
R
Zhang
Yi-Wen
YW
Liu
Xue-Mei
XM
Wang
D004224:Diterpenes / Q000032:analysis / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D007477:Ions / Q000737:chemistry; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D029757:Tripterygium / Q000737:chemistry*
D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D007477:Ions