[Allelic state of the molecular marker for the golden nematode (Globodera rostochiensis) resistance gene H1 among Ukrainian and world cultivars of potato (Solanum tuberosum ssp. tuberosum)].
The purpose of our investigation was determination of allelic state of the H1 resistance gene against the pathotypes Ro1 and Ro4 of golden potato cyst nematode (Globodera rostochiensis) among Ukrainian and world potato (Solanum tuberosum ssp. tuberosum) cultivars. The allelic condition of the TG689 marker was determined by PCR with DNA samples isolated from tubers of potato and primers, one pair of which flanks the allele-specific region and the other one was used for the control of DNA quality. Among analyzed 77 potato cultivars the allele of marker associated with the H1-type resistance was found in 74% of Ukrainian and 90% foreign ones although some of those cultivars proved to be susceptible to the golden potato nematode in field. The obtained data confirm the presence of H1-resistance against golden nematode pathotypes Ro1 and Ro4 among the Ukrainian potato cultivars and efficiency of the used marker within the accuracy that has been declared by its authors.
A V
AV
Karelov
L A
LA
Pilipenko
N A
NA
Kozub
R A
RA
Bondus
A U
AU
Borzykh
I A
IA
Sozinov
Ia B
IaB
Blium
A A
AA
Sozinov
D000483:Alleles; D000818:Animals; D017931:DNA Primers; D017343:Genes, Plant; D005819:Genetic Markers; D005838:Genotype; D009348:Nematoda / Q000502:physiology; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000469:parasitology; D057865:Plant Immunity / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011198:Solanum tuberosum / Q000145:classification / Q000235:genetics / Q000276:immunology* / Q000469:parasitology; D014455:Ukraine
D017931:DNA Primers; D005819:Genetic Markers