UDS-test with freon 11 (R-11).
Trichlorofluoromethane, in concentrations of 80, 400, 2 000, 10 000 and 50 000 ppm, was administered to rats of both sexes (Sprague Dawley) by inhalation exposure.2 000 ppm in air (= 11 200 mg/m(3)) amount to twice the MAK value of 1 000 ppm. At exposure times of 4 hours this corresponds to the MAK value defined for an 8 hour workday.Unscheduled DNA synthesis (UDS) was measured in single-cell suspensions of hepatocytes, pulmonary epithelial cells and lymphocytes of the spleen, respectively.In the pulmonary cells concentrations of 2 000, 10 000 and 50 000 ppm of freon 11 lead to a significantly increased mean silver grain count compared to a negative control group.In spleen and liver cells increasing concentrations of R 11 tend to increase the incorporation of thymidine into the DNA of the cells. These changes of the extent of unscheduled DNA synthesis can, however, not be statistically verified.
Allgemeine Unfallversicherungsanstalt, Adalben-Stifter-Straße 65, A-1200, Wien, Austria.
N
N
Winker
W
W
Klein
P
P
Weniger
E
E
Ott
H
H
Hofer