Affinity chromatographic purification of lentil lectin using immobilized yeast cells.
A model system for evaluating macroporous supports containing immobilized whole cells in affinity Chromatographic applications is described. Whole cells were immobilized in a polyacrylamide network in a two-step polymerization process. The affinity system discussed consists of immobilized cells ofSaccharomyces cervisiae in the purification of lentil lectin fromLens culinaris.
Department of Pure and Applied Biochemistry, University of Lund, PO Box 740, S-220 07, Lund, Sweden.
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Ramstorp
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Mattiasson