Assessment of the effectiveness of silver-coated dressing, chlorhexidine acetate (0.5%), citric acid (3%), and silver sulfadiazine (1%) for topical antibacterial effects against the multi-drug resistant Pseudomonas aeruginosa infecting full-skin thickness burn wounds on rats.
The aim of this study was to compare the effects of four different topical antimicrobial dressings on a multi-drug resistant Pseudomonas aeruginosa contaminated full-thickness burn wound rat model. A total of 40 adult male Wistar albino rats were used. The control group (group 1), silver sulfadiazine (1%) group 2, chlorhexidine acetate (0.5%) group 3, citric acid (3%) group 4, and silver-coated dressing group 5 were compared to assess the antibacterial effects of a daily application to a 30% full-skin thickness burn wound seeded 10 minutes earlier with 10(8) CFU (colony forming unit)/0.5 mL of a multi-drug resistant Pseudomonas aeruginosa strain. Five groups (1 control group and 4 treatment groups) were compared. The administration of third-degree burns to all rats was confirmed based on histopathologic data. The tissue cultures from groups 2 and 5 exhibited significant differences compared to those of the other 3 groups, whereas no significant differences were observed between groups 1, 3, and 4. The effectiveness of the treatments was as follows: 1% silver sulfadiazine > silver-coated dressing > 3% citric acid > 0.5% chlorhexidine acetate > control group. Our results supported the efficacy of topical therapy by silver sulfadiazine and silver-coated dressing on infections caused by multi-drug resistant Pseudomonas spp.
1 Department of Surgery, Baskent University, Ankara, Turkey.
Hakan
H
Yabanoglu
Ozgur
O
Basaran
Cem
C
Aydogan
Ozlem Kurt
OK
Azap
Feza
F
Karakayali
Gokhan
G
Moray
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001458:Bandages; D002056:Burns / Q000382:microbiology*; D002710:Chlorhexidine / Q000494:pharmacology*; D019343:Citric Acid / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial; D008297:Male; D011552:Pseudomonas Infections / Q000188:drug therapy*; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D051381:Rats; D012834:Silver / Q000494:pharmacology*; D012837:Silver Sulfadiazine / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D000891:Anti-Infective Agents, Local; D019343:Citric Acid; D012834:Silver; D002710:Chlorhexidine; D012837:Silver Sulfadiazine