Exploring intercellular signaling by proteomic approaches.
Cells live in a close social context by having mutual communication with their local microenvironment. This complex intercellular communication activates dynamic signaling pathways and regulates specific cell fate. MS-based proteomics has been approved to be inevitable for characterizing dynamic protein expression and PTMs on a global scale. However, because of technical difficulties for targeting membrane receptors and secreted proteins, especially in a physiologically relevant manner, systematic characterization of intercellular signaling by MS-based proteomics has largely lagged behind. Here, I will review the latest proteomics technology development and its application to characterizing different modes of intercellular communication including indirect and direct cell-cell communication, and protein translocalization. I will discuss how MS-based proteomics has been applied for systems-level profiling intercellular signaling in defined biological contexts including tumor microenvironment, bacteria/virus-host cell interaction, immune cell interaction, and stem cell niche.
Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada.
Ruijun
R
Tian
D002450:Cell Communication / Q000235:genetics*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D011499:Protein Processing, Post-Translational / Q000235:genetics; D040901:Proteomics; D015398:Signal Transduction
D036341:Intercellular Signaling Peptides and Proteins
Intercellular signaling; MS; PTM; Protein-protein interaction; Technology