Improved detection limits for electrospray ionization on a magnetic sector mass spectrometer by using an array detector.
Array detection was compared with point detection for solutions of hen egg-white lysozyme, equine myoglobin, and ubiquitin analyzed by electrospray ionization with a magnetic sector mass spectrometer. The detection limits for samples analyzed by using the array detector system were at least 10 times lower than could be achieved by using a point detector on the same mass spectrometer. The minimum detectable quantity of protein corresponded to a signal-to-background ratio of approximately 2∶1 for a 500 amol/μL solution of hen egg-white lysozyme. However, the ultimate practical sample concentrations appeared to be in the 10-100 fmol/μL range for the analysis of dilute solutions of relatively pure proteins or simple mixtures.
JEOL USA, Inc., 11 Dearborn Road, 01960, Peabody, MA.
R B
RB
Cody
J
J
Tamura
J W
JW
Finch
B D
BD
Musselman