Characterization of a bifunctional O- and N-glucosyltransferase from Vitis vinifera in glucosylating phenolic compounds and 3,4-dichloroaniline in Pichia pastoris and Arabidopsis thaliana.
2,4,5-Trichlorophenol, 2,6-dimethylphenol, 3-methylcatechol, phenol, hydroquinone, catechol, and 3,4-dichloroaniline are present in the environment and are risky to humans and animals because of their wide applications in many industries. In this study, a putative uridine diphosphate glucose-dependent glycosyltransferase from Vitis vinifera (VvUGT72B1) displayed high O-glucosyltransferase or N-glucosyltransferase activity toward all these xenbiotics and was able to enhance the resistance of P. pastoris to them. Compared with wild-type Arabidopsis plants, VvUGT72B1-transgenic Arabidopsis plants showed higher resistance to all the xenobiotics except for phenol and exhibited higher removal efficiencies against all xenobiotics. Glucosides of 3-methylcatechol, 2,6-dimethylphenol, phenol, and 3,4-dichloroaniline were exported to the surrounding media by Arabidopsis plants while transgenic Arabidopsis plants exported more glucosides than wild-type Arabidopsis plants. Our findings have the potential to provide a broader spectrum remediation strategy for the phytoremoval and degradation of phenolic compounds and 3,4-dichloroaniline than previous works.
Agro-Biotechnology Research Center, Shanghai Academy of Agricultural Sciences, Shanghai, China ; College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Zhi-Sheng
ZS
Xu
Wei
W
Xue
Ai-Sheng
AS
Xiong
Ya-Qiu
YQ
Lin
Jing
J
Xu
Bo
B
Zhu
Wei
W
Zhao
Ri-He
RH
Peng
Quan-Hong
QH
Yao
D000814:Aniline Compounds / Q000494:pharmacology*; D017360:Arabidopsis / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D010636:Phenols / Q000494:pharmacology*; D010843:Pichia / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D027843:Vitis / Q000201:enzymology*
D000814:Aniline Compounds; D010636:Phenols; C014464:3,4-dichloroaniline; D005964:Glucosyltransferases