HNA-3 gene frequencies in Brazilians and a new polymerase chain reaction-restriction fragment length polymorphism method for HNA-3a/3b genotyping.
BACKGROUND
HNA-3 antigens are the result of a rs2288904 single-nucleotide polymorphism (SNP) in the CTL2, and the HNA-3a and HNA-3b variants are encoded by a guanine and adenine at Nucleotide Position 461. Anti-HNA-3 are involved in severe transfusion-related acute lung injury reactions and in neonatal alloimmune neutropenia. Since the distribution of the HNA-3 system was unknown in South Americans, in this study we determined the frequency of the HNA-3 alleles in Brazilians.


METHODS
DNA of 500 blood donors, 120 Xikrin Amerindians, 74 Japanese individuals, and 124 African Brazilians were genotyped for rs2288904 by a polymerase chain reaction (PCR)-restriction fragment length polymorphism assay. The PCR product was digested with enzyme Taq(α) 1, specific to nucleotide guanine (HNA-3a).


RESULTS
The results showed that the frequencies of the HNA-3a/HNA-3b alleles were 0.81/0.19 in blood donors, 1.00/0.00 in Amerindians, 0.63/0.37 in Japanese, and 0.85/0.15 in African Brazilians. All 81 individuals genotyped as HNA-3a/a did not present the SNP c.457T by molecular sequencing.


CONCLUSIONS
The frequencies of HNA-3 genotypes in Brazilian blood donors is similar to that described in Caucasians; however, all Amerindians were HNA-3a/a, African Brazilians showed a lower frequency of HNA-3b/b, and Japanese had a higher prevalence of HNA-3b/b, suggesting that they may be at risk for developing anti-HNA-3a alloantibodies.
Universidade Federal de São Paulo-UNIFESP, São Paulo, Brazil.
Larissa Barbosa
LB
Lopes
Wilson
W
Baleotti
Rodrigo Buzinaro
RB
Suzuki
Antonio
A
Fabron
Akemi Kuroda
AK
Chiba
João Paulo Botelho
JP
Vieira-Filho
Bianca
B
de Souza Castro
Alessandra
A
Midori Kunioshi
José Orlando
JO
Bordin
D000483:Alleles; D044466:Asian People / Q000235:genetics; D005787:Gene Frequency / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007198:Indians, North American / Q000235:genetics; D007519:Isoantigens / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D044465:White People / Q000235:genetics
C518134:HNA-3a antigen, human; D007519:Isoantigens