Flexible and elastic scaffolds for cartilage tissue engineering prepared by stereolithography using poly(trimethylene carbonate)-based resins.
The aim of this study is to investigate the applicability of flexible and elastic poly(trimethylene carbonate) (PTMC) structures prepared by stereolithography as scaffolds for cartilage tissue engineering. A three-armed methacrylated PTMC macromer with a molecular weight of 3100 g mol(-1) is used to build designed scaffolds with a pore diameter of 350 ± 12 μm and a porosity of 54.0 ± 2.2%. Upon seeding of bovine chondrocytes in the scaffolds, the cells adhere and spread on the PTMC surface. After culturing for 6 weeks, also cells with a round morphology are present, indicative of the differentiated chondrocyte phenotype. Sulphated glycosaminoglycans and fibrillar collagens are deposited by the cells. During culturing for 6 weeks, the compression moduli of the constructs increases 50% to approximately 100 kPa.
MIRA Institute for Biomedical Engineering and Technical Medicine, Department of Biomaterials Science and Technology, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands.
Sigrid
S
Schüller-Ravoo
Sandra M
SM
Teixeira
Jan
J
Feijen
Dirk W
DW
Grijpma
André A
AA
Poot
D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D002356:Cartilage / Q000166:cytology / Q000187:drug effects; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D004146:Dioxanes / Q000737:chemistry*; D004548:Elasticity; D024022:Fibrillar Collagens / Q000096:biosynthesis / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis / Q000378:metabolism; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry*; D064347:Microscopy, Electrochemical, Scanning; D055668:Photochemical Processes; D018583:Pliability; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D004146:Dioxanes; D024022:Fibrillar Collagens; D006025:Glycosaminoglycans; D008689:Methacrylates; D011108:Polymers; C059299:polytrimethylene carbonate
biodegradable scaffolds; chondrocytes; poly(trimethylene carbonate) macromers; stereolithography; tissue engineering