RFLP markers linked to the root knot nematode resistance gene Mi in tomato.
The Mi gene originating from the wild tomato species Lycopersicon peruvianum confers resistance to all major root knot nematodes (Meloidogyne spp.). This single dominant gene is located on chromosome 6 and is very closely linked to the acid phosphatase-1 (Aps-1) locus. Resistance to nematodes has been introgressed into various cultivars of the cultivated tomato (L. esculentum), in many cultivars along with the linked L. peruvianum Aps-1 (1) allele. By using a pair of nearly isogenic lines differing in a small chromosomal region containing the Mi and Aps-1 loci, we have identified two RFLP markers, GP79 and H6A2c2, which are located in the introgressed L. peruvianum region. Analysis of a test panel of 51 L. esculentum genotypes of various origins indicated that GP79 is very tightly linked to the Mi gene and allows both homozygous and heterozygous nematode-resistant genotypes to be distinguished from susceptible genotypes, irrespective of their Aps-1 alleles. Marker H6A2c2 is linked to the Aps-1 locus and is capable of discriminating between the L. peruvianum Aps-1 (1) allele and the L. esculentum Aps-1 (3) and Aps-1 (+) alleles. In combination, these RFLP markers may provide a powerful tool in breeding tomatoes for nematode resistance.
Department of Molecular Biology, Agricultural University, Dreijenlaan 3, NL-6703, HA Wageningen, The Netherlands.
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Klein-Lankhorst
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Rietveld
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B
Machiels
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Verkerk
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R
Weide
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C
Gebhardt
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M
Koornneef
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