Generation of a tenascin-C-CreER2 knockin mouse line for conditional DNA recombination in renal medullary interstitial cells.
Renal medullary interstitial cells (RMIC) are specialized fibroblast-like cells that exert important functions in maintaining body fluid homeostasis and systemic blood pressure. Here, we generated a RMIC specific tenascin-C promoter driven inducible CreER2 knockin mouse line with an EGFP reporter. Similar as endogenous tenascin-C expression, the reporter EGFP expression in the tenascin-C-CreER2(+/-) mice was observed in the inner medulla of the kidney, and co-localized with COX2 but not with AQP2 or AQP1, suggesting selective expression in RMICs. After recombination (tenascin-C-CreER2(+/-)/ROSA26-lacZ(+/-) mice + tamoxifen), β-gal activity was restricted to the cells in the inner medulla of the kidney, and didn't co-localize with AQP2, consistent with selective Cre recombinase activity in RMICs. Cre activity was not obvious in other major organs or without tamoxifen treatment. This inducible RMIC specific Cre mouse line should therefore provide a novel tool to manipulate genes of interest in RMICs.
Gladstone Institute of Virology & Immunology, San Francisco, California, United States of America ; Nephrology Division, Vanderbilt University Medical Center School of Medicine, Nashville, Tennessee, United States of America.
Wenjuan
W
He
Qionghong
Q
Xie
Yingying
Y
Wang
Jing
J
Chen
Min
M
Zhao
Linda S
LS
Davis
Matthew D
MD
Breyer
Guoqiang
G
Gu
Chuan-Ming
CM
Hao
D000818:Animals; D051398:Aquaporin 1 / Q000235:genetics / Q000378:metabolism; D051399:Aquaporin 2 / Q000235:genetics / Q000378:metabolism; D003433:Crosses, Genetic; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D018703:Founder Effect; D005786:Gene Expression Regulation / Q000187:drug effects; D055879:Gene Knock-In Techniques; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D007679:Kidney Medulla / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D007763:Lac Operon; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D013629:Tamoxifen / Q000494:pharmacology; D019063:Tenascin / Q000235:genetics* / Q000378:metabolism; D019076:Transgenes
C496021:Aqp1 protein, mouse; C496024:Aqp2 protein, mouse; D051399:Aquaporin 2; D019063:Tenascin; C485184:enhanced green fluorescent protein; D013629:Tamoxifen; D051398:Aquaporin 1; D049452:Green Fluorescent Proteins; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; C045073:Cre recombinase; D019426:Integrases