Calcium-mediated modulation of Pseudomonas mendocina NR802 biofilm influences the phenanthrene degradation.
A potential biofilm forming and phenanthrene utilizing marine bacterium Pseudomonas mendocina NR802 was isolated from Rushukulya, Odisha, East Coast of India. The effect of Ca(2+) and Mg(2+) on biofilm growth and phenanthrene degradation was evaluated. Among the various tested concentrations, 20 mM of Ca(2+) and Mg(2+) showed a significant enhancement in biofilm production by the bacterium. The SEM-EDAX study showed that the elemental composition of the biofilm varied significantly when grown in the presence of Ca(2+) and Mg(2+). The CSLM analysis of biofilms grown in the presence of 20 mM Ca(2+) and Mg(2+) reveal the critical role of these ions on biofilm architectural parameters such as total biomass, biofilm thickness, roughness coefficient and surface to biovolume ratio. Ca(2+) was found to enhance the extracellular polymeric substances (EPS) production and phenanthrene degradation. Ca(2+) enhanced the biofilm growth in a dose dependent manner, whereas Mg(2+) significantly increased the cell growth in biofilm. More than 15% increase in phenanthrene degradation was observed when biofilm was grown in the presence of an additional 20 mM Ca(2+). This study also supports the fundamental role of Ca(2+) in biofilm growth, architecture as well as biofilm-mediated pollutant degradation.
Laboratory of Environmental Microbiology & Ecology (LEnME), Department of Life Science, National Institute of Technology, Rourkela 769008, Odisha, India.
Neelam
N
Mangwani
Biofouling & Biofilm Processes Section, Water & Steam Chemistry Division, BARC Facilities, Kalpakkam 603102, Tamil Nadu, India.
Sudhir K
SK
Shukla
Biofouling & Biofilm Processes Section, Water & Steam Chemistry Division, BARC Facilities, Kalpakkam 603102, Tamil Nadu, India.
T Subba
TS
Rao
Laboratory of Environmental Microbiology & Ecology (LEnME), Department of Life Science, National Institute of Technology, Rourkela 769008, Odisha, India. Electronic address: surajit@nitrkl.ac.in.
Surajit
S
Das
D001673:Biodegradation, Environmental / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002118:Calcium / Q000494:pharmacology*; D007477:Ions; D008274:Magnesium / Q000494:pharmacology; D008969:Molecular Sequence Data; D010616:Phenanthrenes / Q000378:metabolism*; D044203:Pseudomonas mendocina / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology* / Q000648:ultrastructure; D013052:Spectrometry, X-Ray Emission
D007477:Ions; D010616:Phenanthrenes; C031181:phenanthrene; D008274:Magnesium; D002118:Calcium
Biofilm; Degradation; Extracellular polymeric substances; Phenanthrene; Roughness coefficient