Raman spectroscopic evidence of tissue restructuring in heat-induced tissue fusion.
Heat-induced tissue fusion via radio-frequency (RF) energy has gained wide acceptance clinically and here we present the first optical-Raman-spectroscopy study on tissue fusion samples in vitro. This study provides direct insights into tissue constituent and structural changes on the molecular level, exposing spectroscopic evidence for the loss of distinct collagen fibre rich tissue layers as well as the denaturing and restructuring of collagen crosslinks post RF fusion. These findings open the door for more advanced optical feedback-control methods and characterization during heat-induced tissue fusion, which will lead to new clinical applications of this promising technology.
Department of Surgery and Cancer, Imperial College London, St. Mary's Hospital, London W2 1NY, UK; Department of Electrical Engineering & Electronics, University of Liverpool, Liverpool L69 3GJ, UK.
Lei
L
Su
Kristy L
KL
Cloyd
Shobhit
S
Arya
Martin A B
MA
Hedegaard
Joseph A M
JA
Steele
Daniel S
DS
Elson
Molly M
MM
Stevens
George B
GB
Hanna
D000818:Animals; D006358:Hot Temperature; D007421:Intestine, Small / Q000166:cytology* / Q000528:radiation effects / Q000601:surgery*; D008853:Microscopy; D011846:Radio Waves; D013059:Spectrum Analysis, Raman; D013552:Swine
Raman imaging; Raman spectroscopy; tissue diagnostics; tissue fusion