Production and immunogenicity of VP2 protein of porcine parvovirus expressed in Pichia pastoris.
Viral protein 2 (VP2) of porcine parvovirus (PPV) is the major viral structural protein and is responsible for eliciting neutralizing antibodies in immunized animals. In this study, we constructed and characterized a recombinant yeast vector encoding the VP2 protein, designated as pGAPZαA-VP2. The construct was confirmed by restriction enzyme digestion, PCR, and sequencing and then introduced into P. pastoris strain SMD1168 by electroporation. The expressed VP2 protein was analyzed by SDS-PAGE and western blot. Immunization of mice with the VP2 protein elicited a PPV-specific humoral immune response. Notably, a preparation of VP2 protein containing adjuvant induced a much better antibody response than VP2 alone. Clearly, the adjuvant strongly enhanced the immunogenicity of VP2. This study provides a foundation for the application of the VP2 protein in the clinical diagnosis of PPV and in vaccination against PPV in the future.
State Key Laboratory of Biocontrol, Guangzhou Higher Education Mega Center, School of Life Sciences, Sun Yat-sen University, North Third Road, Guangzhou, Guangdong, 510006, People's Republic of China, 419839537@qq.com.
Chunhe
C
Guo
Zemin
Z
Zhong
Yumao
Y
Huang
D000818:Animals; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D049109:Cell Proliferation; D005260:Female; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D028323:Parvovirus, Porcine / Q000235:genetics / Q000378:metabolism*; D010843:Pichia / Q000378:metabolism*; D011897:Random Allocation; D014765:Viral Vaccines / Q000276:immunology
D036022:Capsid Proteins; D014765:Viral Vaccines