A thermoreversible poly(choline phosphate) based universal biomembrane adhesive.
A new monomer, 2-(2-(2-(2-azidoethoxy)ethoxy)ethoxy)ethyl methacrylate (AEO4 MA), and its direct atom transfer radical polymerization (ATRP) into poly(AEO4 MA), then "clicked" with prop-2-ynyle choline phosphate (CP) to produce a poly(choline phosphate) are described. This polymer exhibits a lower critical solution temperature (LCST) at ≈ 32 °C, and provides a universal thermally reversible biomembrane adhesive, which can rapidly both bind to any mammalian cell membrane and internalize into the cytoplasm of nucleated cells below the LCST. Moving above the LCST reverses cell surface binding. The use of ATRP implies that such polymers can be applied to modify the surfaces of a wide range of biomaterials. The capacity to bind and immobilize cells at room temperature and release them above the LCST should be particularly useful for in vitro cell manipulation and tissue engineering applications.
Centre for Blood Research, 2350 Health Sciences Mall, University of British Columbia, Vancouver, V6T 1Z3, Canada; Department of Pathology and Laboratory Medicine, 2211 Wesbrook Mall, UBC, Vancouver, V6T 2B5, Canada.
Xifei
X
Yu
Xiaoqiang
X
Yang
Sonja
S
Horte
Jayachandran N
JN
Kizhakkedathu
Donald E
DE
Brooks
D000269:Adhesives / Q000138:chemical synthesis* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects*; D018914:Cells, Immobilized; D057930:Click Chemistry; D004912:Erythrocytes / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D005456:Fluorescent Dyes; D005609:Free Radicals; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D044367:Phase Transition; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000494:pharmacology; D058105:Polymerization; D011109:Polymethacrylic Acids / Q000138:chemical synthesis*; D013696:Temperature
D000269:Adhesives; D005456:Fluorescent Dyes; D005609:Free Radicals; D008689:Methacrylates; D011109:Polymethacrylic Acids; D010767:Phosphorylcholine
LCST; biomembrane adhesive; cell immobilization; choline phosphate