Apoptotic cell death and altered calcium homeostasis caused by frataxin depletion in dorsal root ganglia neurons can be prevented by BH4 domain of Bcl-xL protein.
Friedreich ataxia (FRDA) is a neurodegenerative disease characterized by a decreased expression of the mitochondrial protein frataxin. Major neurological symptoms of the disease are due to degeneration of dorsal root ganglion (DRG) sensory neurons. In this study we have explored the neurodegenerative events occurring by frataxin depletion on primary cultures of neurons obtained from rat DRGs. Reduction of 80% of frataxin levels in these cells was achieved by transduction with lentivirus containing shRNA silencing sequences. Frataxin depletion caused mitochondrial membrane potential decrease, neurite degeneration and apoptotic cell death. A marked increase of free intracellular Ca(2+) levels and alteration in Ca(2+)-mediated signaling pathways was also observed, thus suggesting that altered calcium homeostasis can play a pivotal role in neurodegeneration caused by frataxin deficiency. These deleterious effects were reverted by the addition of a cell-penetrant TAT peptide coupled to the BH4, the anti-apoptotic domain of Bcl-x(L). Treatment of cultured frataxin-depleted neurons with TAT-BH4 was able to restore the free intracellular Ca(2+) levels and protect the neurons from degeneration. These observations open the possibility of new therapies of FRDA based on modulating the Ca(2+) signaling and prevent apoptotic process to protect DRG neurons from neurodegeneration.
Grup de Bioquímica de L'Estrès Oxidatiu, Departament de Ciències Mèdiques Bàsiques, IRB Lleida, Universitat de Lleida, Lleida, Spain.
Stefka
S
Mincheva-Tasheva
Elia
E
Obis
Jordi
J
Tamarit
Joaquim
J
Ros
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000235:genetics; D002478:Cells, Cultured; D005621:Friedreich Ataxia / Q000235:genetics; D005727:Ganglia, Spinal / Q000166:cytology*; D015696:Gene Products, tat / Q000235:genetics; D006706:Homeostasis; D033862:Iron-Binding Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000235:genetics; D024101:Mitochondrial Proteins / Q000378:metabolism; D016501:Neurites / Q000473:pathology; D019636:Neurodegenerative Diseases; D017434:Protein Structure, Tertiary / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering; D051381:Rats; D011984:Sensory Receptor Cells / Q000166:cytology*; D051020:bcl-X Protein / Q000235:genetics*; D000096923:Frataxin
D002118:Calcium; D015696:Gene Products, tat; D033862:Iron-Binding Proteins; D024101:Mitochondrial Proteins; D034741:RNA, Small Interfering; D051020:bcl-X Protein; D000096923:Frataxin; C494813:Bcl2l1 protein, rat