The detection and elimination of flavin-containing monooxygenase 3 gene T329S mutation in the Beijing You chicken.
In this study, using a newly developed TaqMan-based real-time PCR method for the T329S mutation in the flavin-containing monooxygenase 3 (FMO3) gene, a marker-assisted selective breeding program against the unfavorable T allele was implemented in the Beijing You chicken breeding stock from 2010 to 2012. A total of 2,359 breeder candidate chickens were detected. After 1-generation culling in both males and females and 2-generation culling only in males, genotyping results in 2013 showed that there still remained a low unfavorable allele frequency of 0.022 in this population. The results indicated that to ensure a complete eradication of the defective tainting mutation in FMO3 out of the Beijing You population, more strict breeding and management schemes should be carried out in the future.
Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Qin
Q
Chu
Jian
J
Zhang
Shanshan
S
Zhu
Yao
Y
Zhang
Haihong
H
Wang
Ailian
A
Geng
Huagui
H
Liu
D000818:Animals; D030161:Avian Proteins / Q000235:genetics* / Q000378:metabolism; D001947:Breeding; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005787:Gene Frequency; D060005:Genotyping Techniques / Q000379:methods* / Q000662:veterinary; D008297:Male; D009154:Mutation; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism; D012150:Polymorphism, Restriction Fragment Length; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods* / Q000662:veterinary; D012641:Selection, Genetic
D030161:Avian Proteins; D010105:Oxygenases; C020066:dimethylaniline monooxygenase (N-oxide forming)