[Study of drug intervention in diversity changes of TCRVbeta gene in AIDS patients with incomplete immune reconstitution].
OBJECTIVE
To discuss the drug intervention in diversity changes of TCRVbeta gene in AIDS patients with incomplete immune reconstitution.


METHODS
PBMCs were isolated from 37 cases of AIDS patients failure to immune reconstitution before and after treatment with immune 2 and 15 cases of HIV negative healthy donors. The human gene TCRVbeta CDR3 diversity quantitative detection reagent box were used, and mapped the distribution of gene scanning and calculated different CDR3 fragme of each Vbeta family size.


RESULTS
Compared with the normal group, there appeared some single or oligoclonal amplification of Vbeta CDR3 region in the patients, which were improved or recovered after treatment. Among them, D value of four families (9, 11, 21, 22 ) decreased after treatment in both groups. The decrease in family 21 and 22 was significant (P < 0.05) in treatment group compared with the control group. And family 18 was decreased in treatment group and increased significantly in control group (P < 0.05).


CONCLUSIONS
Study of the mechanism showed oligoclonal of TCRVbeta family can get recovery in some degrees after treated by Immune 2 plus HAART, suggesting that the medicine may promote T-cell receptor gene rearrangement, helping immune cells to effectively identify the virus to reduce T-cell apoptosis.
Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China. wangjie0103@yahoo.com.cn
Jie
J
Wang
Yan-Li
YL
Tang
Yong
Y
Li
Yong-Mei
YM
Liu
Yi
Y
Zeng
D000163:Acquired Immunodeficiency Syndrome / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology*; D023241:Antiretroviral Therapy, Highly Active; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D014644:Genetic Variation / Q000187:drug effects*; D006801:Humans; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*
D004365:Drugs, Chinese Herbal; D011948:Receptors, Antigen, T-Cell