Expression of a chimaeric kanamycin resistance gene introduced into the wild soybeanGlycine canescens using a cointegrate Ri plasmid vector.
Seedling hypocotyl explants ofGlycine canescens were inoculated withAgrobacterium rhizogenes carrying a chimaeric NPTII gene cointegrated into the TL-DNA of pRiA4. Transformed roots produced shoots on B5 based medium with 10.0 mgl(-1) BAP, 0.05 mgl(-1) IBA and 50 μgml(-1) kanamycin. Cultured roots and regenerated plants expressed NPTII enzyme activity which was correlated with the presence of Ri TL-DNA and the structural sequence of the NPTII gene.
Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, University Park, Nottingham, UK.
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Rech
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Golds
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Husnain
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Vainstein
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Jones
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Hammatt
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Mulligan
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Davey