Protective and reparative effects of peptides from soybean β-conglycinin on mice intestinal mucosa injury.
Peptides derived from alcalase digestion of soybean β-conglycinin, containing 8.52% carbohydrate, exhibits an inhibition effect on pathogen adhesion or translocation to intestinal cells in vitro. In this study, the protective and reparative effects of β-conglycinin peptides on intestinal mucosa injury in vivo were studied using mice with dextran sulfate sodium (DSS)-induced intestinal mucosa injury. The results showed that β-conglycinin peptides contained approximately 21.77% glutamic acid (Glu), and significantly reduced the histological injury in mice both in the protective and reparative experiments. The myeloperoxidase activity of mice treated with β-conglycinin peptides decreased compared with those treated DSS in the positive control group. Immunohistochemical analysis also showed that β-conglycinin peptides inhibited the expression of inflammatory factor NF-κB/p65. These results suggested that peptides derived from soybean β-conglycinin exhibited protective and reparative effects on mice intestinal mucosa injury.
College of Food Science and Nutritional Engineering, China Agricultural University , Haidian District, Beijing , China and.
Jianhua
J
Ren
Baichong
B
Yang
Ying
Y
Lv
Shuntang
S
Guo
D000818:Animals; D052179:Antigens, Plant / Q000494:pharmacology / Q000627:therapeutic use*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D016264:Dextran Sulfate; D005260:Female; D005916:Globulins / Q000494:pharmacology / Q000627:therapeutic use*; D018836:Inflammation Mediators / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology / Q000627:therapeutic use*; D055314:Seed Storage Proteins / Q000494:pharmacology / Q000627:therapeutic use*; D030262:Soybean Proteins / Q000494:pharmacology / Q000627:therapeutic use*; D013025:Glycine max / Q000737:chemistry*
D052179:Antigens, Plant; D005916:Globulins; D018836:Inflammation Mediators; D016328:NF-kappa B; D010455:Peptides; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; D016264:Dextran Sulfate