[Discussion on the quality marker of rehmanniae radix praeparata in the Chinese Pharmacopoeia].
OBJECTIVE
In the Chinese Pharmacopoeia (2010 edition), the minimum limit of verbascoside (acteoside) in Rehmanniae Radix Praeparata (RRP) was set at 0.20%, the rationality of the standard was evaluated in this paper.


METHODS
10 samples of Rehmanniae Radix (RR) and RRP were collected or prepared. According to the traditional method, different samples of RRP were prepared from the same batch of RR, and the content of verbascoside was determined by HPLC.


RESULTS
The average content of verbascoside in RR and RRP was 0.0226% and 0.0097%, respectively, and their difference was up to the level of P < 0.01. The content of verbascoside was found decreased with processing time increasing. Long-time storage also results in substantial loss of verbascoside.


CONCLUSIONS
Processing results in verbascoside decreasing significantly. It is unreasonable that RR and RRP have the same standard in the content of verbascoside. It is suggested that the maximum limit of verboscoside should be set in the new version of Chinese pharmacopoeia.
Institute of Pharmacognosy, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China. wgdong2008@126.com
Guo-Dong
GD
Wei
Huan
H
Liu
Xue-Sen
XS
Wen
Yan-Xia
YX
Guo
D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards*; D005960:Glucosides / Q000032:analysis*; D006801:Humans; D010602:Pharmacopoeias as Topic; D010636:Phenols / Q000032:analysis*; D011786:Quality Control; D012016:Reference Values; D032267:Rehmannia / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods; D059808:Polyphenols
D004365:Drugs, Chinese Herbal; D005960:Glucosides; D010636:Phenols; C058956:acteoside; D059808:Polyphenols