Fluorescence labeling and interaction of atherogenic lipoproteins with cultured cells.
We prepared lipoprotein (a) and LDL covalently labeled with either BODIPY or rhodamine. A dual wavelength method was used for the microscopic observation of both lipoproteins during their interaction with HepG2 cells. Since a large proportion of Lp(a) colocalized with LDL on the cell surface and inside the cells, it was concluded that Lp(a) uptake into cells is mediated by LDL via internalization of LDL.
Department of Biochemistry and Food Chemistry, Technische Universität Graz, Petersgasse 12, A-8010, Graz, Austria.
G
G
Hofer
R
R
Gorges
G M
GM
Kostner
F
F
Paltauf
A
A
Hermetter