Iloprost reduces colitis induced oxidative stress: An experimental study in rats.
OBJECTIVE
Reactive oxygen species have a known potent role in the pathogenesis of ulcerative colitis. Iloprost, a pharmaceutical, is a chemically stable derivative of a naturally- occurring human prostacyclin. Several studies have demonstrated protective effects of iloprost via its antioxidant and its anti-inflammatory activity. The aim of this study is to evaluate the effects of iloprost on oxidant/antioxidant status, as well as the large bowel histopathology in experimental colitis.


METHODS
Forty adult male Wistar-albino rats were randomly divided in to four equal weight-matched groups: sham group (n=10), iloprost administered sham group (n=10), colitis group (n=10), iloprost administered colitis group (n=10). Acetic acid (1 ml of 4% solution) was used to induce colonic inflammation in the rats.


RESULTS
Colonic tissue and plasma malondialdehyde levels were significantly lower in the iloprost administered colitis group than the colitis group (p<0.01). Tissue glutathione levels of the iloprost administered colitis group were significantly higher than the colitis group (p<0.001).


CONCLUSIONS
We have demonstrated in this study iloprost to be an antioxidant, as well as iloprost demonstrating protective activity against colitis induced oxidative stress.
İstanbul University Cerrahpaşa Medical Faculty, Department of General Surgery, İstanbul, Turkey.
Erman
E
Aytaç
Serkan
S
Teksöz
Seha
S
Saygılı
Osman Baran
OB
Tortum
Nihat
N
Yavuz
Volkan
V
Sözer
Süha
S
Göksel
Hafize
H
Uzun
Hakkı Oktay
HO
Seymen
Ertuğrul
E
Göksoy
D019342:Acetic Acid; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D003106:Colon / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D016285:Iloprost / Q000494:pharmacology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione; D016285:Iloprost; D019342:Acetic Acid