T-DNA length variability in mannopine hairy root: more than 50 kilobasepairs of pRi T-DNA can integrate in plant cells.
Independent carrot (Daucus carota) hairy root lines were established by inoculation of discs taken from the same carrot with Agrobacterium rhizogenes 8196 and A. tumefaciens C58C1(pRi8196) carrying pRi8196. Several lines were compared with respect to T-DNA length. One of them was found to have integrated sequences covering more than 50 kbp of the Ri plasmid.
Groupe de Recherche sur les Interactions entre Microorganismes et Plantes, INRA et UA 136 CNRS, Institut de Microbiologie, Bâtiment 409, F-91405, Orsay, France.
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