[Sequencing and analysis of the complete mitochondrial DNA of Chinese moccasin for medicinal use].
OBJECTIVE
To sequence and analyze the complete mitochondrial DNA of Chinese moccasin.


METHODS
The circular 17 541 bp sequence of mitochondrial DNA (mtDNA) of Chinese moccasin was determined by Ex-Taq PCR, TA-cloning and primer-walking methods.


RESULTS
This mitogenome contained 37 coding genes (including 22 tRNA, 2 rRNA and 13 protein-coding genes) and two control regions (CR and psiCR). The gene content and arrangement of Chinese moccasin mtDNA was similar to those of other vipers reported so far.


CONCLUSIONS
The characteristics of the replication origin and the tRNA arrangement of snake mitochondrial genomes can be used for identification of medicinal snakes.
Zhongshan Torch Polytechnic, Zhongshan 528436, China.
Nian
N
Chen
Shen-Gan
SG
Gao
Xiao-Ping
XP
Lai
Peng
P
Liu
Xin
X
Wang
Ling-Li
LL
Wang
D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics; D054629:Genome, Mitochondrial / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA; D012911:Snakes / Q000235:genetics*
D004272:DNA, Mitochondrial; D012335:RNA, Ribosomal; D012343:RNA, Transfer