Vanilloid receptor-1 (TRPV1) expression and function in the vasculature of the rat.
Transient receptor potential (TRP) cation channels are emerging in vascular biology. In particular, the expression of the capsaicin receptor (TRPV1) was reported in vascular smooth muscle cells. This study characterized the arteriolar TRPV1 function and expression in the rat. TRPV1 mRNA was expressed in various vascular beds. Six commercially available antibodies were tested for TRPV1 specificity. Two of them were specific (immunostaining was abolished by blocking peptides) for neuronal TRPV1 and one recognized vascular TRPV1. TRPV1 was expressed in blood vessels in the skeletal muscle, mesenteric and skin tissues, as well as in the aorta and carotid arteries. TRPV1 expression was found to be regulated at the level of individual blood vessels, where some vessels expressed, while others did not express TRPV1 in the same tissue sections. Capsaicin (a TRPV1 agonist) evoked constrictions in skeletal muscle arteries and in the carotid artery, but had no effect on the femoral and mesenteric arteries or the aorta. In blood vessels, TRPV1 expression was detected in most of the large arteries, but there were striking differences at level of the small arteries. TRPV1 activity was suppressed in some isolated arteries. This tightly regulated expression and function suggests a physiological role for vascular TRPV1.
Division of Clinical Physiology, Institute of Cardiology, University of Debrecen, Debrecen, Hungary (AT, AC, ETP, IR, ISM, IE, IE, ZP).
Attila
A
Tóth
Agnes
A
Czikora
Eniko T
ET
Pásztor
Beatrix
B
Dienes
Péter
P
Bai
László
L
Csernoch
Ibolya
I
Rutkai
Viktória
V
Csató
Ivetta S
IS
Mányiné
Róbert
R
Pórszász
István
I
Edes
Zoltán
Z
Papp
Judit
J
Boczán
D000818:Animals; D001158:Arteries / Q000378:metabolism; D002211:Capsaicin / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000098:blood supply; D008297:Male; D008643:Mesentery / Q000098:blood supply; D009119:Muscle Contraction / Q000187:drug effects; D018482:Muscle, Skeletal / Q000098:blood supply; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D009417:Nerve Tissue / Q000098:blood supply; D009928:Organ Specificity; D051381:Rats; D011921:Rats, Inbred WKY; D012867:Skin / Q000098:blood supply; D013116:Spinal Cord / Q000098:blood supply; D050916:TRPV Cation Channels / Q000819:agonists / Q000378:metabolism*; D014680:Veins / Q000378:metabolism
D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat; D002211:Capsaicin
arteriolar constriction; capsaicin; dorsal root ganglia; functional; resistance artery; vanilloid receptor-1 (TRPV1); vascular biology