RBP-J in FOXD1+ renal stromal progenitors is crucial for the proper development and assembly of the kidney vasculature and glomerular mesangial cells.
The mechanisms underlying the establishment, development, and maintenance of the renal vasculature are poorly understood. Here, we propose that the transcription factor "recombination signal binding protein for immunoglobulin kappa J region" (RBP-J) plays a key role in the differentiation of the mural cells of the kidney arteries and arterioles, as well as the mesangial cells of the glomerulus. Deletion of RBP-J in renal stromal cells of the forkhead box D1 (FOXD1) lineage, which differentiate into all the mural cells of the kidney arterioles along with mesangial cells and pericytes, resulted in significant kidney abnormalities and mortality by day 30 postpartum (P30). In newborn mutant animals, we observed a decrease in the total number of arteries and arterioles, along with thinner vessel walls, and depletion of renin cells. Glomeruli displayed striking abnormalities, including a failure of FOXD1-descendent cells to populate the glomerulus, an absence of mesangial cells, and in some cases complete loss of glomerular interior structure and the development of aneurysms. By P30, the kidney malformations were accentuated by extensive secondary fibrosis and glomerulosclerosis. We conclude that RBP-J is essential for proper formation and maintenance of the kidney vasculature and glomeruli.
MR4 Bldg. Rm. 2001, Univ. of Virginia School of Medicine, 409 Lane Rd., Charlottesville, VA 22908. rg@virginia.edu.
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EE
Lin
M L S
ML
Sequeira-Lopez
R A
RA
Gomez
D000818:Animals; D000831:Animals, Newborn; D001160:Arterioles / Q000166:cytology; D002452:Cell Count; D051858:Forkhead Transcription Factors / Q000502:physiology*; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein / Q000378:metabolism*; D007150:Immunohistochemistry; D007668:Kidney / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D007678:Kidney Glomerulus / Q000166:cytology; D050527:Mesangial Cells / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D012079:Renal Circulation / Q000502:physiology; D013234:Stem Cells / Q000502:physiology*; D017154:Stromal Cells / Q000502:physiology*
D051858:Forkhead Transcription Factors; C489562:Foxd1 protein, mouse; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; C497571:Rbpj protein, mouse
development; fibrosis; mesangial cell; renal arterioles; renin; vasculature