Effects of resveratrol on vitrified porcine oocytes.
Vitrified MII porcine oocytes are characterized by reduced developmental competence, associated with the activation of the apoptotic pathway. Resveratrol (R), a polyphenolic compound present in several vegetal sources, has been reported to exert, among all its other biological effects, an antiapoptotic one. The aim of this study was to determine the effects of R (2  µM) on the apoptotic status of porcine oocytes vitrified by Cryotop method, evaluating phosphatidylserine (PS) exteriorization and caspases activation. R was added during IVM (A); 2 h postwarming incubation (B); vitrification/warming and 2 h postwarming incubation (C); all previous phases (D). Data on PS exteriorization showed, in each treated group, a significantly higher (P < 0.05) percentage of live nonapoptotic oocytes as compared with CTR; moreover, the percentage of live apoptotic oocytes was significantly (P < 0.05) lower in all R-treated groups relative to CTR. The results on caspase activation showed a tendency to an increase of viable oocytes with inactive caspases in B, C, and D, while a significant (P < 0.05) increase in A compared to CTR was recorded. These data demonstrate that R supplementation in various phases of IVM and vitrification/warming procedure can modulate the apoptotic process, improving the resistance of porcine oocytes to cryopreservation-induced damage.
Dipartimento di Scienze Mediche Veterinarie (DIMEVET), Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, Bologna, Italy.
Elisa
E
Giaretta
Marcella
M
Spinaci
Diego
D
Bucci
Carlo
C
Tamanini
Giovanna
G
Galeati
D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D001562:Benzimidazoles / Q000378:metabolism; D020169:Caspases / Q000378:metabolism; D015925:Cryopreservation; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D010718:Phosphatidylserines / Q000378:metabolism; D011419:Propidium / Q000378:metabolism; D000077185:Resveratrol; D013194:Staining and Labeling; D013267:Stilbenes / Q000494:pharmacology*; D034421:Sus scrofa
D017304:Annexin A5; D001562:Benzimidazoles; D010718:Phosphatidylserines; D013267:Stilbenes; D011419:Propidium; D020169:Caspases; C017807:bisbenzimide ethoxide trihydrochloride; D000077185:Resveratrol