Production of theanine and other γ-glutamyl derivatives by Camellia sinensis cultured cells.
The maximum theanine production by Camellia sinensis cultured cells was achieved by culturing in the modified MS medium containing 2 mg/l indole-3-butyric acid, 0.1 mg/l kinetin, 0 mM NH4NO3 and 39.6 mM KNO3 with 40 mM ethylamine hydrochloride or 20 mM ethylamine hydrochloride and 10 mM L-glutamic acid. Other primary amines, such as methylamine, n-butylamine, 2-hydroxyethylamine, 2cyanoethylamine, aniline, benzylamine and phenylethylamine, were also biotransformed to N(5)-alkyl-L-glutamine derivatives by C. sinensis cultured cells.
School of Pharmaceutical Sciences, Kitasato University, Minato-ku, 108, Tokyo, Japan.
Y
Y
Orihara
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T
Furuya