Detection of ginkgolide A in Ginkgo biloba cell cultures.
Ginkgo biloba cells were cultured in two 500 mL shake flasks and in 2 L and 6 L immobilization bioreactors using MS medium supplemented with 1 mg.L(-1) NAA, 0.1 mg.L(-1) K and 30 g.L(-1) sucrose. Specific growth rates were 0.06 d(-1), 0.11 d(-1) and 0.07 d(-1) for the 2 L and 6 L bioreactors and shake flask cultures, respectively. Extracellular phosphate, nitrate, ammonium and carbohydrate uptake rates of the bio reactor cultures were approximately 17 to 39% slower than those of shake flask cultures. The specific oxygen uptake and carbon dioxide transfer rates of immobilized Ginkgo biloba cells ranged from 0.027 to 0.041 mmol O2.g(-1).d.w.hr(-1) (maximum uptake at 14 days) and 0.020 to 0.057 mmol CO2g. (-1).d.w.hr(-1) (maximum production at 14 days). Extracts from the biomass of the two immobilized and shake flask suspension cultures were analysed for ginkgolide A by GC-MS. Yields of 7, 17, 19 and 7 ng.g. (-1)d.w. of ginkgolide A were determined for shake flask 1, shake flask 2 and the 2 L and 6 L immobilized cultures, respectively. Traces of ginkgolide B were detected with the signal to noise ratio, however, being too low for positive confirmation of this last product.
Chemical Engineering Department, McGill University, 3480 University St., H3A 2A7, Montreal, Canada.
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DJ
Carrier
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Chauret
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Mancini
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Coulombe
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Neufeld
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Weber
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Archambault