A high-throughput method for the simultaneous determination of multiple mycotoxins in human and laboratory animal biological fluids and tissues by PLE and HPLC-MS/MS.
A high-throughput method for the determination of 28 mycotoxins involving pressurised liquid extraction (PLE) coupled with liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has been optimised and validated for determination in various biological fluids and tissues of human and laboratory animals. High-throughput analysis was achieved using PLE pre-treatment and without the need for any cleanup. The extraction solvent was acetonitrile/water/acetic acid (80/19/1, v/v/v). The static extraction time was 5min. The extraction pressure and temperature were 1500psi and 140°C, respectively. The flush volume was 60%. The limits of detection, which were defined as CCα, varied from 0.01μg/kg (μg/L) to 0.69μg/kg (μg/L). The recoveries of spiked samples from 0.20μg/kg (μg/L) to 2μg/kg (μg/L) ranged from 71% to 100.5% with relative standard deviations of less than 17.5%, except FB1 and FB2 recoveries, which were lower than 60%. The method was successfully applied in real samples, and the data indicate that this technique is a useful analytical method for the determination of mycotoxins from humans and animals. To the best of our knowledge, this method is the first for the large-scale testing of multi-class mycotoxins in all types of biological fluids and tissues that uses PLE and HPLC-MS/MS.
Department of Pharmacy, Wuhan University, Wuhan 430072, People's Republic of China.
Xiaoqin
X
Cao
Shuangchan
S
Wu
Yuan
Y
Yue
Shi
S
Wang
Yuting
Y
Wang
Li
L
Tao
Hui
H
Tian
Jianmei
J
Xie
Hong
H
Ding
D000293:Adolescent; D000328:Adult; D000653:Amniotic Fluid / Q000737:chemistry; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005243:Feces / Q000737:chemistry; D005260:Female; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D059625:Liquid-Liquid Extraction / Q000379:methods*; D008297:Male; D008875:Middle Aged; D008895:Milk, Human / Q000737:chemistry; D009183:Mycotoxins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D011247:Pregnancy; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D055815:Young Adult
D009183:Mycotoxins
Biological fluids and tissues; Humans; LC–MS/MS; Laboratory animals; Multiple mycotoxins; PLE