Enteric YaiW is a surface-exposed outer membrane lipoprotein that affects sensitivity to an antimicrobial peptide.
yaiW is a previously uncharacterized gene found in enteric bacteria that is of particular interest because it is located adjacent to the sbmA gene, whose bacA ortholog is required for Sinorhizobium meliloti symbiosis and Brucella abortus pathogenesis. We show that yaiW is cotranscribed with sbmA in Escherichia coli and Salmonella enterica serovar Typhi and Typhimurium strains. We present evidence that the YaiW is a palmitate-modified surface exposed outer membrane lipoprotein. Since BacA function affects the very-long-chain fatty acid (VLCFA) modification of S. meliloti and B. abortus lipid A, we tested whether SbmA function might affect either the fatty acid modification of the YaiW lipoprotein or the fatty acid modification of enteric lipid A but found that it did not. Interestingly, we did observe that E. coli SbmA suppresses deficiencies in the VLCFA modification of the lipopolysaccharide of an S. meliloti bacA mutant despite the absence of VLCFA in E. coli. Finally, we found that both YaiW and SbmA positively affect the uptake of proline-rich Bac7 peptides, suggesting a possible connection between their cellular functions.
School of Medicine and Dentistry, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom.
Markus F F
MF
Arnold
Paola
P
Caro-Hernandez
Karen
K
Tan
Giulia
G
Runti
Silvia
S
Wehmeier
Marco
M
Scocchi
William T
WT
Doerrler
Graham C
GC
Walker
Gail P
GP
Ferguson
D023181:Antimicrobial Cationic Peptides / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D002003:Brucella abortus / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D016153:Genes, Suppressor; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D016962:Sinorhizobium meliloti / Q000235:genetics; D014158:Transcription, Genetic; D014166:Transferases / Q000235:genetics / Q000378:metabolism
D023181:Antimicrobial Cationic Peptides; D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D008074:Lipoproteins; C586515:YaiW protein, E coli; C586516:YaiW protein, Salmonella typhimurium; C078379:pyrH protein, E coli; D014166:Transferases