Erythroid differentiation is augmented in Reelin-deficient K562 cells and homozygous reeler mice.
Reelin is an extracellular glycoprotein that is highly conserved in mammals. In addition to its expression in the nervous system, Reelin is present in erythroid cells but its function there is unknown. We report in this study that Reelin is up-regulated during erythroid differentiation of human erythroleukemic K562 cells and is expressed in the erythroid progenitors of murine bone marrow. Reelin deficiency promotes erythroid differentiation of K562 cells and augments erythroid production in murine bone marrow. In accordance with these findings, Reelin deficiency attenuates AKT phosphorylation of the Ter119(+)CD71(+) erythroid progenitors and alters the cell number and frequency of the progenitors at different erythroid differentiation stages. A regulatory role of Reelin in erythroid differentiation is thus defined.
Graduate Institute of Biomedical Science, College of Medicine, Chang Gung University, Kwei-Shan, Taoyuan 333, Taiwan, ROC.
Hui-Chun
HC
Chu
Graduate Institute of Biomedical Science, College of Medicine, Chang Gung University, Kwei-Shan, Taoyuan 333, Taiwan, ROC.
Hsing-Ying
HY
Lee
Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Kwei-Shan, Taoyuan 333, Taiwan, ROC.
Yen-Shu
YS
Huang
Graduate Institute of Biomedical Science, College of Medicine, Chang Gung University, Kwei-Shan, Taoyuan 333, Taiwan, ROC.
Wei-Lien
WL
Tseng
Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Kwei-Shan, Taoyuan 333, Taiwan, ROC.
Ching-Ju
CJ
Yen
Department of Medical Laboratory Sciences and Biotechnology, China Medical University, Taichung 404, Taiwan, ROC. Electronic address: jccheng@mail.cmu.edu.tw.
Ju-Chien
JC
Cheng
Graduate Institute of Biomedical Science, College of Medicine, Chang Gung University, Kwei-Shan, Taoyuan 333, Taiwan, ROC; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Kwei-Shan, Taoyuan 333, Taiwan, ROC; Molecular Medicine Research Center, Chang Gung University, Kwei-Shan, Taoyuan 333, Taiwan, ROC. Electronic address: ctseng@mail.cgu.edu.tw.
Ching-Ping
CP
Tseng
D000818:Animals; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000378:metabolism; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D019070:Cell Lineage / Q000235:genetics; D041905:Erythroid Cells / Q000378:metabolism*; D015672:Erythroid Precursor Cells / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D015973:Gene Expression Regulation, Leukemic; D006720:Homozygote; D006801:Humans; D020014:K562 Cells; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008818:Mice, Neurologic Mutants; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034622:RNA Interference; D000091042:Reelin Protein; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D000091042:Reelin Protein; D051057:Proto-Oncogene Proteins c-akt; C000717127:RELN protein, human; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases
3,3′,5,5′-tetramethylbenzidine; EPO; Erythroid differentiation; FBS; HRP; Hb; Hct; K562 cell; MCH; MCHC; MCV; NFAT; NaB; PAGE; PBS; PVDF; RDW; RPMI; Reeler mice; Reelin; Roswell Park Memorial Institute; SDS; Sodium butyrate; TMB; erythropoietin; fetal bovine serum; hematocrit; hemoglobin; horseradish peroxidase; mean corpuscular hemoglobin; mean corpuscular hemoglobin concentration; mean corpuscular volume; nuclear factor of activated T cells; phosphate-buffered saline; polyacrylamide gel electrophoresis; polyvinylidene fluoride; red blood cell distribution width; sodium butyrate; sodium dodecyl sulfate