Enhanced methanol production in plants provides broad spectrum insect resistance.
Plants naturally emit methanol as volatile organic compound. Methanol is toxic to insect pests; but the quantity produced by most of the plants is not enough to protect them against invading insect pests. In the present study, we demonstrated that the over-expression of pectin methylesterase, derived from Arabidopsis thaliana and Aspergillus niger, in transgenic tobacco plants enhances methanol production and resistance to polyphagous insect pests. Methanol content in the leaves of transgenic plants was measured using proton nuclear spectroscopy (1H NMR) and spectra showed up to 16 fold higher methanol as compared to control wild type (WT) plants. A maximum of 100 and 85% mortality in chewing insects Helicoverpa armigera and Spodoptera litura larvae was observed, respectively when fed on transgenic plants leaves. The surviving larvae showed less feeding, severe growth retardation and could not develop into pupae. In-planta bioassay on transgenic lines showed up to 99 and 75% reduction in the population multiplication of plant sap sucking pests Myzus persicae (aphid) and Bemisia tabaci (whitefly), respectively. Most of the phenotypic characters of transgenic plants were similar to WT plants. Confocal microscopy showed no deformities in cellular integrity, structure and density of stomata and trichomes of transgenic plants compared to WT. Pollen germination and tube formation was also not affected in transgenic plants. Cell wall enzyme transcript levels were comparable with WT. This study demonstrated for the first time that methanol emission can be utilized for imparting broad range insect resistance in plants.
CSIR-National Botanical Research Institute, Council of Scientific and Industrial Research, Lucknow, Uttar Pradesh, India ; Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2-Rafi Marg, New Delhi, India.
Sameer
S
Dixit
Santosh Kumar
SK
Upadhyay
Harpal
H
Singh
Om Prakash
OP
Sidhu
Praveen Chandra
PC
Verma
Chandrashekar
C
K
D000818:Animals; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics; D001234:Aspergillus niger / Q000201:enzymology / Q000235:genetics; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism; D002473:Cell Wall / Q000201:enzymology; D005822:Genetic Vectors / Q000235:genetics; D007313:Insecta / Q000502:physiology*; D000432:Methanol / Q000378:metabolism*; D010641:Phenotype; D030821:Plants, Genetically Modified; D014026:Nicotiana / Q000166:cytology / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D014170:Transformation, Genetic; D019076:Transgenes / Q000235:genetics
D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase; D000432:Methanol