In vitro propagation through axillary bud multiplication in different mulberry genotypes.
Axillary buds from 5 genotypes of mulberry belonging to 4 species were cultured on modified MS basal medium. A total of 30 media combinations were tried for all the genotypes. The response of axillary buds and the requirement for growth regulators varied with genotype. In Morus indica BAP (0.25-0.5 mg/l), and in M. alba and M. rotondifolia GA3 (0.5-1.0 mg/l)were found to induce sprouting. Two genotypes of M. bombycis, namely Schimanochi and Mizusawa, developed healthy shoots on the incorporation of 2,4-D (0.5-1.0 mg/l) and BAP (0.5-2.0 mg/l), respectively. IBA (0.5 mg/l), along with cytokinin/auxin/gibberellin, had no effect on bud growth but helped root induction. Shoots developed from the axillary buds were further multiplied as nodal explants. MS basal medium supplemented with 0.5 mg/l IBA and LS vitamins was found best to produce healthy plantlets in all the genotypes. An average 89% survival was observed on transferring the plantlets to soil.
Tissue Culture Laboratory, Mulberry Breeding and Genetics Section, Central Sericultural Research and Training Institute, 570008, Mysore, India.
A K
AK
Jain
S B
SB
Dandin
K
K
Sengupta