Fibroblast growth factor acts upon the transcription of phospholipase C genes in human umbilical vein endothelial cells.
Besides the control of calcium levels, the phosphoinositide-specific phospholipases C (PI-PLCs), the main players in the phosphoinositide signalling pathway, contribute to a number of cell activities. The expression of PI-PLCs is strictly tissue specific and evidence suggests that it varies under different conditions, such as tumour progression or cell activation. In previous studies, we obtained a complete panel of expression of PI-PLC isoforms in human umbilical vein endothelial cells (HUVEC), a widely used experimental model for endothelial cells (EC), and demonstrated that the expression of the PLC genes varies under inflammatory stimulation. The fibroblast growth factor (FGF) activates the PI-PLC γ1 isoform. In the present study, PI-PLC expression in FGF-treated HUVEC was performed using RT-PCR, observed 24 h after stimulation. The expression of selected genes after stimulation was perturbed, suggesting that FGF affects gene transcription in PI signalling as a possible mechanism of regulation of its activity upon the AkT-PLC pathway. The most efficient effects of FGF were recorded in the 3-6-h interval. To understand the complex events progressing in EC might provide useful insights for potential therapeutic strategies. The opportunity to manipulate the EC might offer a powerful tool of considerable practical and clinical importance.
Dipartimento Organi di Senso, Policlinico Umberto I, Facoltà di Medicina e Odontoiatria, Università di Roma "Sapienza", viale del Policlinico 155, 00185, Rome, Italy, ritalovasco@hotmail.it.
Vincenza Rita
VR
Lo Vasco
Martina
M
Leopizzi
Chiara
C
Puggioni
Carlo
C
Della Rocca
Rita
R
Businaro
D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D005786:Gene Expression Regulation; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology*; D006801:Humans; D007249:Inflammation / Q000235:genetics; D054803:Phospholipase C delta / Q000235:genetics*; D051966:Phospholipase C gamma / Q000235:genetics*; D020033:Protein Isoforms / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects
D020033:Protein Isoforms; D012333:RNA, Messenger; D005346:Fibroblast Growth Factors; D054803:Phospholipase C delta; D051966:Phospholipase C gamma