Staphylococcus aureus enterotoxin B-induced endoplasmic reticulum stress response is associated with chronic rhinosinusitis with nasal polyposis.
OBJECTIVE
Staphylococcus aureus enterotoxin B (SEB) might participate in the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). However, the exact mechanism of polyp formation in CRSwNP remains unclear. Since the endoplasmic reticulum (ER) stress response is closely associated with chronic inflammation, we investigated the association between ER stress and SEB in the pathogenesis of CRSwNP.


METHODS
Twenty-three CRSwNP patients with eosinophilic polyps (EP) or non-eosinophilic polyps (NEP) and 10 healthy subjects who were undergoing septoplasty were enrolled in this study. ER stress response was investigated using immunohistochemical staining and Western blotting.


RESULTS
We show in this study that there are significantly more SEB-positive cells and higher production of reactive oxygen species (ROS) in the epithelial layer of EP than NEP or control tissue. Both SEB and protein A were detected strongly in tissues from patients with CRSwNP. We observed SEB induced the ER stress response in RPMI 2650 cells. GRP78 elevation by SEB was reduced by ROS scavenger pretreatment. In addition, the induction of GRP78 and p47 phox was increased significantly in EP compared with NEP or control mucosa.


CONCLUSIONS
SEB may induce ER stress via ROS production in CRSwNP. Therefore, we suggest that SEB-induced ER stress may play important roles in the pathogenesis of nasal polyposis.
Department of Otorhinolaryngology-Head and Neck Surgery, Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon 301-721, South Korea.
Yong-Min
YM
Kim
Department of Otorhinolaryngology-Head and Neck Surgery, Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon 301-721, South Korea; Department of Otorhinolaryngology, Yanbian University Hospital, Jilin Yanji 133000, China.
Jun
J
Jin
Department of Microbiology, Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon 301-747, South Korea; Infection Signaling Network Research, College of Medicine, Chungnam National University, Daejeon 301-747, South Korea.
Ji-Ae
JA
Choi
Department of Microbiology, Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon 301-747, South Korea; Infection Signaling Network Research, College of Medicine, Chungnam National University, Daejeon 301-747, South Korea.
Soo-Na
SN
Cho
Department of Microbiology, Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon 301-747, South Korea; Infection Signaling Network Research, College of Medicine, Chungnam National University, Daejeon 301-747, South Korea.
Yun-Ji
YJ
Lim
Department of Microbiology, Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon 301-747, South Korea; Infection Signaling Network Research, College of Medicine, Chungnam National University, Daejeon 301-747, South Korea.
Jung-Hwan
JH
Lee
Department of Microbiology, Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon 301-747, South Korea; Infection Signaling Network Research, College of Medicine, Chungnam National University, Daejeon 301-747, South Korea.
Jee Yeon
JY
Seo
Department of Pathology, Yanbian University Hospital, Jilin Yanji 133000, China.
Hai Yue
HY
Chen
Department of Otorhinolaryngology-Head and Neck Surgery, Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon 301-721, South Korea.
Ki-Sang
KS
Rha
Department of Microbiology, Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon 301-747, South Korea; Infection Signaling Network Research, College of Medicine, Chungnam National University, Daejeon 301-747, South Korea. Electronic address: songch@cnu.ac.kr.
Chang-Hwa
CH
Song
D016022:Case-Control Studies; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D004768:Enterotoxins / Q000633:toxicity*; D006801:Humans; D009298:Nasal Polyps / Q000378:metabolism / Q000473:pathology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012852:Sinusitis / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000302:isolation & purification*
D000091342:Endoplasmic Reticulum Chaperone BiP; D004768:Enterotoxins; C000717247:HSPA5 protein, human; D017382:Reactive Oxygen Species; C031818:enterotoxin B, staphylococcal
ER stress; Nasal polyposis; Staphylococcus aureus enterotoxin B