[Study on extraction and purification technology of Hubei ophiopogon saponins].
OBJECTIVE
To explore the extraction and purification technology of total saponins from the effective parts of Liriope spicata.


METHODS
Orthogonal design was used. Macroporous resin was selected to separate and purify total saponin from the effective parts of Liriope spicata. The process validation was conducted. The total saponins was determined by Ultraviolet Spectrophotometry.


RESULTS
The optimal extraction conditions were as follows: 10 times the amount of ethanol (70%) for each occasion and hot reflux (3 x 2 h). Total saponins was purified by D101 macroporous resin. The concentration of eluting ethanol was 50% - 70%. Ethanol (70%) was selected as the best eluent. The result of process validation was consistent with the study.


CONCLUSIONS
The process is simple and stable enough to significantly improve the extraction rate of the effective parts. The study can provide reference for the research and production of effective parts of traditional Chinese medicine such as Liriope spicata.
School of Chemical Engineering, Wuhan University of Techology, Wuhan 430070, China.
Yun-Han
YH
Lin
Chong-Ming
CM
Li
Xiao-Dong
XD
Li
Yang
Y
Xiang
Ya-Qin
YQ
Zhang
Xiao-Cun
XC
Zhang
Xia
X
Liu
D002681:China; D000431:Ethanol / Q000737:chemistry; D031441:Ophiopogon / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D016062:Porosity; D011786:Quality Control; D012117:Resins, Synthetic / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors
D012117:Resins, Synthetic; D012503:Saponins; D000431:Ethanol