Chiral separation-based ligand exchange by open-tubular capillary electrochromatography.
Chiral ligand-exchange enantioseparation of aliphatic and aromatic amino acids was successfully performed using a new open-tubular zwitterionic column with tentacle-type polymer stationary phase. The polymeric stationary phase was prepared using 3-chloro-2-hydroxypropyl methacrylate (HPMA-Cl), a new reactive monomer. The preparation procedure of the open-tubular column included silanization, in situ graft polymerization with HPMA-Cl, and L-histidine (L-His) modification. L-His was used as a chiral ligand-exchange selector and copper(II) as a central ion. Successful enantioseparation of six pairs of amino acid enantiomers was achieved with a buffer of 5 mM CuSO₄, 20 mM (NH4)₂SO₄ at pH 3.0.
Biochemistry Division, Department of Chemistry, Hacettepe University, 06342 Beytepe-Ankara, Turkey; Department of Food Engineering, Bingöl University, Bingöl, Turkey. Electronic address: aydoganc@hacettepe.edu.tr.
Cemil
C
Aydoğan
Biochemistry Division, Department of Chemistry, Hacettepe University, 06342 Beytepe-Ankara, Turkey.
Adil
A
Denizli
D024322:Amino Acids, Aromatic / Q000737:chemistry / Q000302:isolation & purification; D053801:Capillary Electrochromatography / Q000379:methods*; D008024:Ligands; D011108:Polymers / Q000737:chemistry; D013237:Stereoisomerism
D024322:Amino Acids, Aromatic; D008024:Ligands; D011108:Polymers
Amino acid enantioseparation; HPMA-Cl; Ligand exchange; Open-tubular CEC