Embryogenesis and plant regeneration from anther culture of bamboo (Sinocalamus latiflora (Munro) McClure).
Embryogenic callus was initiated from bamboo (Sinocalumus satiflora (Munro) McClure) anthers cultured on N6 medium supplemented with 1 mg/l 2,4-D, 1 mg/l BA, 2 g/l charcoal, 0.8% agar (Sigma) and 9% sucrose. Anthers with microspores at miduninucleate to early-binucleate stages showed better rate of response for callus induction. Prolonged culture of these embryogenic calli on the original medium or subculture to an auxin-free medium resulted in embryoid formation and their subsequent germination to form rooted plantlets. Chromosome counts from root-tip cells of anther-derived plant indicated that they were haploid (N=36).
Department of Agronomy, Taiwan Agricultural Research Institute, Taichung, Taiwan 41301, Republic of China.
H S
HS
Tsay
C C
CC
Yeh
J Y
JY
Hsu