Multistep DNA-templated synthesis using a universal template.
We report a DNA-templated synthesis method that allows construction of the entire DNA-encoded library with a single DNA template. Taking advantage of deoxyinosine's indiscriminate base-pairing property, we designed a "universal template" that is capable of directing chemical reactions with multiple reactant DNAs with different sequences. In combination with other design features including photocleavable linkers and direct encoding by the reactant DNA, we demonstrated the capabilities of the universal template in library synthesis, target selection, and hit decoding. Our method can be generally and straightforwardly applied to prepare a variety of chemically diverse DNA-encoded libraries.
Key Laboratory of Bioorganic Chemistry and Molecular Engineering of the Ministry of Education, Beijing National Laboratory of Molecular Sciences (BNLMS), College of Chemistry and Molecular Engineering, Peking University , 202 Chengfu Road, Beijing, China 100871.
Yizhou
Y
Li
Peng
P
Zhao
Mingda
M
Zhang
Xianyuan
X
Zhao
Xiaoyu
X
Li
D020029:Base Pairing; D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D015723:Gene Library; D008969:Molecular Sequence Data
D004247:DNA