Gold nanosponges: green synthesis, characterization, and cytotoxicity.
A simply approach for the synthesis of Au nanostructures in tea infusions at room temperature is developed. By controlling the concentrations of tea infusions, Au nanostructures in various shapes and sizes have been prepared. From 1 x (original concentration) and 0.01 x (100 times diluted) tea infusions, 52.2 +/- 8.1 nm Au nanosponges (T-Au NSs) and 23 +/- 2 nm spherical Au nanoparticles (T-Au NPs) were prepared. The phytochemicals present on the surface of T-Au NSs were proved by surface-assisted laser desorption/ionization mass spectrometry, Fourier transform infrared spectrometry and capillary electrophoresis coupled with UV detection. The energy-dispersive X-ray spectroscopy and powder X-ray diffraction data reveal pure crystalline structures of the T-Au NSs. The dark field scattering images observe that the T-Au NSs have significant affinity toward HeLa cells. The cytotoxicity of T-Au NSs on HeLa cells is through caspase-3 activation.
Department of Chemistry, National Changhua University of Education, Changhua 500, Taiwan.
Yang-Wei
YW
Lin
Yi-Cheng
YC
Chen
Chia-Wei
CW
Wang
Wen-Tsen
WT
Chen
Chao-Ming
CM
Liu
Chung-Yu
CY
Chen
Huan-Tsung
HT
Chang
D053148:Caspase 3 / Q000378:metabolism; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002470:Cell Survival; D006046:Gold / Q000737:chemistry*; D006801:Humans; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures; D013056:Spectrophotometry, Ultraviolet
D006046:Gold; D053148:Caspase 3