Ratio of "A-type" to "B-type" proanthocyanidin interflavan bonds affects extra-intestinal pathogenic Escherichia coli invasion of gut epithelial cells.
Gut colonization by extra-intestinal pathogenic Escherichia coli (ExPEC) increases the risk of subsequent infections, including urinary tract infection and septicemia. Previous work suggests that cranberry proanthocyanidins (PAC) interact with bacterial surface factors, altering bacterial interaction with host cells. Methods were developed to determine if ratios of "A-type" to "B-type" interflavan bonds in PAC affect ExPEC agglutination and invasion of enterocytes. In cranberries, 94.5% of PAC contain one or more "A-type" bonds, whereas in apples, 88.3% of PAC contain exclusively "B-type" bonds. Results show that cranberry "A-type" PAC have greater bioactivity than apple "B-type" PAC for increasing ExPEC agglutination and decreasing ExPEC epithelial cell invasion.
Department of Food Science, University of Wisconsin-Madison , 1605 Linden Drive, Madison, Wisconsin 53706, United States.
Rodrigo P
RP
Feliciano
Jennifer J
JJ
Meudt
Dhanansayan
D
Shanmuganayagam
Christian G
CG
Krueger
Jess D
JD
Reed
D018938:Caco-2 Cells; D004847:Epithelial Cells / Q000382:microbiology*; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology; D004927:Escherichia coli Infections / Q000382:microbiology*; D006801:Humans; D007422:Intestines / Q000166:cytology / Q000382:microbiology; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D044945:Proanthocyanidins / Q000737:chemistry* / Q000494:pharmacology*; D029799:Vaccinium macrocarpon / Q000737:chemistry*
D010936:Plant Extracts; D044945:Proanthocyanidins; C013221:proanthocyanidin
Caco-2; ExPEC; agglutination; apple; cranberry; invasion; proanthocyanidins; “A-type” bonds; “B-type” bonds