A simplified culture system to examine soluble factor interactions between mammalian cells.
Interactions between different cell types play critical roles in normal development and disease, but remain challenging to analyse. Here, a co-culture system is described that overcomes many of the limitations of existing methods, is simple to construct and modify, and is compatible with standard cellular and molecular assays.
University of Wisconsin-Madison, Department of Biomedical Engineering, 2154 Engineering Centers Building, 1550 Engineering Drive, Madison, WI 53711, USA. kreeger@wisc.edu.
Molly J
MJ
Carroll
Lauren E
LE
Stopfer
Pamela K
PK
Kreeger
D002448:Cell Adhesion; D002478:Cells, Cultured; D018920:Coculture Techniques; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D005260:Female; D005780:Gelatin / Q000378:metabolism*; D006801:Humans; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D010766:Phosphorylation
D005780:Gelatin; D048049:Extracellular Signal-Regulated MAP Kinases