Resolution of multiexponential spectral relaxation of Yt-base by global analysis of collisionally quenched samples.
We measured the wavelength-dependent intensity decays of 4,9-dihydro-4,6-dimethyl-9-oxo-1H-imidazo-1,2a-purine (Yt-base) in propanol to determine the time-resolved emission spectra and rates of spectral relaxation. We found that resolution of the spectral relaxation times was dramatically improved by global analysis of the frequency-domain data with increasing amounts of the collisional quencher CCl4. Collisional quenching preferentially decreases the longer-lived relaxed component of the emission, thereby increasing the fractional contribution of the incompletely relaxed portion of the emission. The data could not be explained by a single spectral relaxation time, and at least two relaxation times are needed to describe the time-dependent emission center of gravity of Yt-base.
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland, School of Medicine, 108 North Greene Street, 21201, Baltimore, Maryland.
H
H
Szmacinski
I
I
Gryczynski
J R
JR
Lakowicz