Universal dark quencher based on "clicked" spectrally distinct azo dyes.
The first synthesis of an heterotrifunctional molecular scaffold derived from the popular DABCYL azo dye quencher has been achieved. The sequential derviatization of this trivalent azobenzene derivative with two other nonfluorescent azo dyes (Black Hole Quencher BHQ-1 and BHQ-3) and through effective reactions from the "bioconjugation chemistry" repertoire has led to an universal dark quencher (UDQ). This "clicked" poly azo dye is able to turn off an array of fluorophores covering the UV/NIR (300-750 nm) spectral range.
Normandie Université , COBRA UMR 6014 & FR 3038, UNIV Rouen, INSA Rouen, CNRS, IRCOF, 1 Rue Tesnières, 76821 Mont-Saint-Aignan Cedex, France , Institut Universitaire de France , 103 Boulevard Saint-Michel, 75005 Paris, France , and Laboratory PBS , UMR 6270 & FR 3038, Bât. Dulong, 76821 Mont-Saint-Aignan Cedex, France.
Arnaud
A
Chevalier
Julie
J
Hardouin
Pierre-Yves
PY
Renard
Anthony
A
Romieu
D001391:Azo Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D012995:Solubility; D014867:Water
D001391:Azo Compounds; D005456:Fluorescent Dyes; D014867:Water; C009850:azobenzene