Inhibitory effect of esterified lactoferin and lactoferrin against tobacco mosaic virus (TMV) in tobacco seedlings.
The inhibitory effects of esterified lactoferrin (ELF) and lactoferrin (LF) against tobacco mosaic virus (TMV) in tobacco seedlings and the underlying mechanism were investigated. ELF and LF significantly inhibited viral infection and TMV multiplication in tobacco plants. ELF showed a higher inhibition effect against TMV than LF treatment in a dose and time-dependent way. Moreover, ELF induced a higher increase in the levels of transcription of pathogenesis-related (PR) protein genes [acidic PRs (PR-1a, PR-2, PR-3, PR-5) and basic PR-1] and defense-related enzymes [phenylalanine ammonia lyase (PAL, EC 4.3.1.5), and 5-epi-aristolochene synthase (EAS, EC 2.5.1.35)] both locally and systemically, in correlation with the induction of resistance against tobacco mosaic virus. Furthermore, ELF also induced accumulation of salicylic acid, SA 2-O-β-D-glucoside and H2O2. These results suggested that ELF and LF could control TMV incidence and the mechanism might attribute to activate the expression of a number of defense genes.
Department of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.
Jie
J
Wang
Hong-Yan
HY
Wang
Xiao-Ming
XM
Xia
Peng-peng
PP
Li
Kai-Yun
KY
Wang
D004951:Esterification; D007781:Lactoferrin / Q000737:chemistry / Q000494:pharmacology*; D010935:Plant Diseases / Q000517:prevention & control / Q000821:virology*; D036226:Seedlings / Q000254:growth & development / Q000821:virology; D014026:Nicotiana / Q000254:growth & development / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000187:drug effects* / Q000502:physiology
D007781:Lactoferrin