A standardized staining protocol for L1CAM on formalin-fixed, paraffin-embedded tissues using automated platforms.
The L1 cell adhesion molecule (L1CAM) is overexpressed in many human cancers and can serve as a biomarker for prognosis in most of these cancers (including type I endometrial carcinomas). Here we provide an optimized immunohistochemical staining procedure for a widely used automated platform (VENTANA™), which has recourse to commercially available primary antibody and detection reagents. In parallel, we optimized the staining on a semi-automated BioGenix (i6000)  immunostainer. These protocols yield good stainings and should represent the basis for a reliable and standardized immunohistochemical detection of L1CAM in a variety of malignancies in different laboratories.
Kaplan Medical Center, Rehovot - Israel.
Mina
M
Fogel
Ayelet
A
Harari
Elisabeth
E
Müller-Holzner
Alain G
AG
Zeimet
Gerhard
G
Moldenhauer
Peter
P
Altevogt
D005260:Female; D005557:Formaldehyde; D006801:Humans; D007150:Immunohistochemistry; D039842:Neural Cell Adhesion Molecule L1 / Q000032:analysis*; D010051:Ovarian Neoplasms / Q000737:chemistry* / Q000473:pathology; D016612:Paraffin Embedding; D016707:Tissue Fixation
D039842:Neural Cell Adhesion Molecule L1; D005557:Formaldehyde