Purification and functional analysis of human properdin.
Properdin is a member of the alternative pathway of complement. It is unique in that it is the only known positive regulator of the complement system. Properdin can stabilize and promote complement activation, but in addition is also capable of initiating the alternative pathway, making it a unique protein. This chapter focuses on the methods required for purifying human properdin and testing its functionality.
Department of Nephrology, Leiden University Medical Centre, Leiden, The Netherlands.
Joseph
J
O'Flynn
Maria C Faber
MC
Krol
Mohamed R
MR
Daha
Cees
C
van Kooten
D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003179:Complement C3b / Q000378:metabolism; D003170:Complement Pathway, Alternative; D006801:Humans; D011414:Properdin / Q000302:isolation & purification* / Q000378:metabolism*; D011485:Protein Binding
D011414:Properdin; D003179:Complement C3b