Natural variation in PTB1 regulates rice seed setting rate by controlling pollen tube growth.
Grain number, panicle seed setting rate, panicle number and grain weight are the most important components of rice grain yield. To date, several genes related to grain weight, grain number and panicle number have been described in rice. However, no genes regulating the panicle seed setting rate have been functionally characterized. Here we show that the domestication-related POLLEN TUBE BLOCKED 1 (PTB1), a RING-type E3 ubiquitin ligase, positively regulates the rice panicle seed setting rate by promoting pollen tube growth. The natural variation in expression of PTB1 which is affected by the promoter haplotype and the environmental temperature, correlates with the rice panicle seed setting rate. Our results support the hypothesis that PTB1 is an important maternal sporophytic factor of pollen tube growth and a key modulator of the rice panicle seed setting rate. This finding has implications for the improvement of rice yield.
1] Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang 611130, Sichuan, China [2] State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu 611130, China [3] Key Laboratory of Crop Genetic Resources and Improvement, Sichuan Agricultural University, Ministry of Education, Ya'an 625014, Sichuan, China [4].
Shuangcheng
S
Li
Wenbo
W
Li
Bin
B
Huang
Xuemei
X
Cao
Xingyu
X
Zhou
Shumei
S
Ye
Chengbo
C
Li
Fengyan
F
Gao
Ting
T
Zou
Kailong
K
Xie
Yun
Y
Ren
Peng
P
Ai
Yangfan
Y
Tang
Xuemei
X
Li
Qiming
Q
Deng
Shiquan
S
Wang
Aiping
A
Zheng
Jun
J
Zhu
Huainian
H
Liu
Lingxia
L
Wang
Ping
P
Li
D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics*; D053205:Pollen Tube / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D012639:Seeds / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D013696:Temperature; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*
D010940:Plant Proteins; D044767:Ubiquitin-Protein Ligases