Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images.
BACKGROUND
The faithful determination of the concentration and viability of yeast cells is important for biological research as well as industry. To this end, it is important to develop an automated cell counting algorithm that can provide not only fast but also accurate and precise measurement of yeast cells.


RESULTS
With the proposed method, we measured the precision of yeast cell measurements by using 0%, 25%, 50%, 75% and 100% viability samples. As a result, the actual viability measured with the proposed yeast cell counting algorithm is significantly correlated to the theoretical viability (R2 = 0.9991). Furthermore, we evaluated the performance of our algorithm in various computing platforms. The results showed that the proposed algorithm could be feasible to use with low-end computing platforms without loss of its performance.


CONCLUSIONS
Our yeast cell counting algorithm can rapidly provide the total number and the viability of yeast cells with exceptional accuracy and precision. Therefore, we believe that our method can become beneficial for a wide variety of academic field and industries such as biotechnology, pharmaceutical and alcohol production.
Applied Computing Lab., GIST, Oryong-dong, Gwangju 500-712, Korea.|Logos Biosystems Inc, Pyungchon-dong, Kyunggi 431-755, Korea.
Dongpyo
D
Hong
Logos Biosystems Inc, Pyungchon-dong, Kyunggi 431-755, Korea.
Gwanghee
G
Lee
Logos Biosystems Inc, Pyungchon-dong, Kyunggi 431-755, Korea.
Neon Cheol
NC
Jung
Applied Computing Lab., GIST, Oryong-dong, Gwangju 500-712, Korea.
Moongu
M
Jeon