MCP-1 promotes invasion and adhesion of human ovarian cancer cells.
BACKGROUND
Monocyte chemoattractant protein-1 (MCP-1) can accelerate tumor progression by attracting tumor-associated macrophages. We studied the effects of MCP-1 on SKOV-3 cells in order to investigate MCP-1 biological activity ovarian cancer.


METHODS
A SKOV-3 cell invasion assay (Transwell assay) and cell adhesion assay (96-well assay) were performed. Immunohistochemical staining for C-C motif chemokine receptor-2 (CCR2), a receptor for MCP-1, was also performed on cultured SKOV-3 cells.


RESULTS
Migration and adhesion of MCP-1-treated SKOV-3 cells were significantly increased compared to untreated cells (p<0.01). A CCR2 antagonist attenuated the invasion and adhesion of MCP-1-treated cells. CCR2 was expressed in the cytoplasm of SKOV-3 cells.


CONCLUSIONS
MCP-1 promoted invasion and adhesion of ovarian cancer cells, and a CCR2 antagonist attenuated the effects of MCP-1 in vitro. These data suggest that MCP-1 is a potential therapeutic target for ovarian cancer therapy.
Department of Obstetrics and Gynecology, Fukushima Medical University School of Medicine, 1-Hikarigaoka, Fukushima City, 960-1295, Japan. s-furu@fmu.ac.jp.
Shigenori
S
Furukawa
Shu
S
Soeda
Yuichiro
Y
Kiko
Osamu
O
Suzuki
Yuko
Y
Hashimoto
Takafumi
T
Watanabe
Hiroshi
H
Nishiyama
Kazuhiro
K
Tasaki
Hiroshi
H
Hojo
Masafumi
M
Abe
Keiya
K
Fujimori
D002448:Cell Adhesion; D002465:Cell Movement; D018932:Chemokine CCL2 / Q000378:metabolism*; D018284:Cystadenocarcinoma, Serous / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D009361:Neoplasm Invasiveness; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology*; D054390:Receptors, CCR2 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014407:Tumor Cells, Cultured
D018932:Chemokine CCL2; D054390:Receptors, CCR2
C-C motif chemokine receptor-2 (CCR2) antagonist; Ovarian cancer; SKOV-3 cell line; monocyte chemoattractant protein-1 (MCP-1); tumor-associated macrophages (TAMs)