Potential probiotic characterization of Lactobacillus plantarum strains isolated from Inner Mongolia "Hurood" cheese.
Total 121 lactic acid bacteria were isolated from homemade Inner Mongolia extra hard Hurood cheese. Seven of these strains, identified as Lactobacillus plantarum, were studied for probiotic characteristics. All seven strains survived at pH 3.0 for 3 h, or in the presence of oxgall at 0.3% or 0.6% for 4 h, but their viabilities were affected to different extents at pH 2.0 for 3 h. Strains C37 and C51 showed better adherence to Caco-2 cells, and higher hydrophobicity. The seven L. plantarum strains were different in in vitro free radical scavenging activities and cholesterolreducing ability. In vivo evaluation of the influence of L. plantarum C37 on the intestinal flora in a mouse model showed strain C37 could increase the viable counts of lactobacilli in feces of mice and decrease the viable counts of enterococci. When L. plantarum C37 was used to prepare probiotic Hurood cheese, it was able to maintain high viable counts (>7.8 log CFU/g) during the whole storage period, but the composition of the cheese was not changed. These results indicate that L. plantarum C37 could be considered as a promising probiotic strain.
School of Biological and Agricultural Engineering, Jilin University, Changchun 130022, P.R. China, Institute of Agro-food Technology, Jilin Academy of Agricultural Sciences, Changchun 130033, P.R. China.
Jian
J
Zhang
Xue
X
Zhang
Li
L
Zhang
Yujuan
Y
Zhao
Chunhua
C
Niu
Zhennai
Z
Yang
Shengyu
S
Li
D000818:Animals; D001422:Bacterial Adhesion; D058448:Biota; D002460:Cell Line; D002611:Cheese / Q000382:microbiology; D002681:China; D002784:Cholesterol / Q000378:metabolism; D003902:Detergents / Q000633:toxicity; D004847:Epithelial Cells / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005609:Free Radicals / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000382:microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D048191:Lactiplantibacillus plantarum / Q000302:isolation & purification* / Q000502:physiology*; D051379:Mice; D050296:Microbial Viability / Q000187:drug effects; D019936:Probiotics / Q000302:isolation & purification*
D003902:Detergents; D005609:Free Radicals; D002784:Cholesterol