Use of eggs derived from the interspecific charr hybrids to induce androgenetic development of the brook charr (Salvelinus fontinalis Mitchill 1814).
Although, brook charr (Salvelinus fontinalis Mitchill 1814) and Arctic charr (Salvelinus alpinus Linnaeus 1758) are able to cross and give fertile offspring, their androgenetic nucleocytoplasmic hybrids are not viable. To overcome incompatibility between the egg cytoplasm of one charr species and the sperm nucleus of another charr species, application of F1 interspecific hybrids as egg donors for the purpose of androgenesis has been proposed. Here, androgenetic development of the brook charr was successfully induced in the brook charr eggs and the eggs derived from the reciprocal brook charr × Arctic charr F1 hybrids. A working androgenesis protocol included inactivation of the maternal nuclear DNA achieved by irradiation of the eggs with 420 Gy of X-rays, insemination of such treated eggs with the haploid sperm cells and exposition of the haploid androgenetic zygotes to the high hydrostatic pressure shock (51.711 MPa for 4 min) applied 420 min after insemination. Androgenetic larvae that hatched from the brook charr and the hybrid eggs were shown to be homozygous brook charr individuals. Androgenetic individuals exhibited 84 chromosomes and 100 chromosome arms (FN), values characteristic for the brook charr diploid cells. Strategy hybridize first than induce androgenesis should be tested in order to provide androgenetic offspring in other salmonids that are able to cross and produce fertile offspring.
Department of Molecular Evolution, University of Gdansk, Gdansk, Poland.
O
O
Michalik
S
S
Dobosz
I
I
Wójcik
T
T
Zalewski
K
K
Ocalewicz
D000818:Animals; D001947:Breeding / Q000379:methods; D020732:Cytogenetic Analysis; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary; D006824:Hybridization, Genetic / Q000502:physiology*; D007246:Infertility / Q000235:genetics*; D008297:Male; D012100:Reproduction, Asexual / Q000502:physiology; D015996:Survival Rate; D014337:Trout / Q000235:genetics* / Q000502:physiology*