[Effect of folate on the proliferation of human cervical cancer cell and relationship with HPV16].
OBJECTIVE
To study the effect of different concentration of folate in vitro on the proliferation and apoptosis of C33A cell with HPV negative and CaSki cell with HPV16 positive, and intreaction of folate and HPV16.


METHODS
C33A and Caski cells were intervened by different concentration of folate (0.1, 1.0, 10, 50, 100, 500, 1,000 and 2,000 microg/ml). The morphological changes of cells were examined by reverse discrepancy microscope. MTT and Flow Cytometry (FCM) were employed to measure the proliferation, inhibition ratio, cell cycle and apoptosis of cells. RT-PCR was used to detect the expression of HPV16 E2/E6 oncogene.


RESULTS
The effects of folate on the C33A and CaSki cell growth suppression were increased gradually with the concentration of folate increasing and cultured duration prolonging, the inhibition ratio was increased (C33A: r = 0.948, P = 0.010; CaSki: r = 0.895, P = 0.006). Adding folate could induce cell apoptosis (C33A: r = 0.989, P < 0.001; CaSki: r =0.994, P < 0.001), and there was a linear relationship, but there was no significant difference on the proliferation and apoptosis of C33A and CaSki cells effected. In addition, concentration of folate did not affect the expression of HPV16 E2/E6.


CONCLUSIONS
Different concentration of folate inhibited cervical cancer cell proliferation and enhanced cell apoptosis, but the results do not support an interaction between folate and HPV16 in cervical cancer cell.
Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, China. dingling79@163.com
Ling
L
Ding
Jingli
J
Ma
Qin
Q
Zhou
Jintao
J
Wang
D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D005492:Folic Acid / Q000494:pharmacology*; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000473:pathology* / Q000821:virology
C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger; D012097:Repressor Proteins; D005492:Folic Acid