Comparison of enrichment broths for the recovery of healthy and heat-injured Salmonella typhimurium on raw duck wings.
This study was performed to optimiz eSalmonella Typhimurium recovery from raw duck wings with five nonselective broths (buffered peptone water, tryptic soy broth, lactose broth, universal preenrichment broth, nutrient broth) and four selective broths (selenite broth, BAX System MP media [MP], Salmonella AD media [AD], ONE broth-Salmonella [OB]). Healthy or heat-injured (50 and 85% injury) cells were inoculated at a level of 10(2), 10(1), or 10(0) CFU/25 g on raw duck wings. Growth was modeled using DMfit with four growth parameters: lag-phase duration, maximum growth rate, doubling time, and maximum population density. Most enrichments were able to recover Salmonella Typhimurium to greater than 6 log CFU/ml. AD, MP, and OB had significantly (P < 0.05) higher maximum growth rate (0.9 to 1.0/h) and lower doubling time (0.7 to 0.8 h). Buffered peptone water, AD, MP, and OB recovered healthy and 50%-injured cells at low inoculum levels to more than 6.0 log CFU/ml; OB achieved the greatest recovery (7.6 and 7.9 log CFU/ml), following 24 h of incubation. The 85%-injured cells at 10(0) and 10(1) CFU/25 g, however, were only recovered in OB, reaching 7.3 and 7.5 log CFU/ml, respectively. These results suggest that OB may be an appropriate enrichment broth for the recovery of Salmonella Typhimurium from raw duck wings in standard diagnostic tests or other rapid detection methods, to avoid false-negative results.
Food Science and Technology Programme, Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543.
Qianwang
Q
Zheng
Caroline
C
Bustandi
Yishan
Y
Yang
Keith R
KR
Schneider
Hyun-Gyun
HG
Yuk
D000818:Animals; D015169:Colony Count, Microbial / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D004372:Ducks / Q000382:microbiology*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D006358:Hot Temperature; D007700:Kinetics; D012486:Salmonella typhimurium / Q000254:growth & development* / Q000302:isolation & purification; D014921:Wings, Animal / Q000382:microbiology
D003470:Culture Media