Polysaccharides in the culture medium of cotton (Gossypium hirsutum L.) ovules cultured in vitro.
Polysaccharides secreted into the culture medium (PCM) from cotton (Gossypium hirsutum L.) ovules, culturedin vitro, have been examined. The amount of the polysaccharides increases with the duration of the culture, but except for the galactose content, does not vary markedly with the culture age. Analysis shows that the polysaccharide mixture contains mainly pectins, but there is also a significant amount of xyloglucan. The source of the polysaccharides is most likely the callus tissue which develops on the ovules.
Institut de Biologie végétale et de Phytochimie, Université de Fribourg, CH-1700, Fribourg, Switzerland.
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Buchala
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Roulin
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Meier