Application of protease-hydrolyzed whey as a complex nitrogen source to increase poly(3-hydroxybutyrate) production from oils by Cupriavidus necator.
Whole whey hydrolyzed by Alcalase (WWH) was tested as a complex nitrogen source for the production of poly(3-hydroxybutyrate) (PHB) from waste frying oils by Cupriavidus necator H16. Addition of WWH (10 % (v/v) of cultivation media) supported the growth and PHB accumulation; PHB yields in Erlenmeyer flasks were more than 3.5-fold higher than in control cultivations. The positive influence of WWH on PHB production was confirmed in experiments performed in laboratory fermentor. C. necator cultivated with WWH produced 28.1 g PHB l(-1) resulting in a very high product yield coefficient of 0.94 g PHB per g oil. Since PHB yields were ~40 % higher than in the control cultivation, WWH can be considered as an excellent inexpensive nitrogen source for PHB production by C. necator.
Faculty of Chemistry, Materials Research Centre, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic, Stana.O@seznam.cz.
Stanislav
S
Obruca
Pavla
P
Benesova
Jana
J
Oborna
Ivana
I
Marova
D003470:Culture Media / Q000737:chemistry*; D020118:Cupriavidus necator / Q000254:growth & development* / Q000378:metabolism*; D006868:Hydrolysis; D006885:Hydroxybutyrates / Q000378:metabolism*; D008894:Milk Proteins / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism*; D011091:Polyesters / Q000378:metabolism*; D013381:Subtilisins / Q000378:metabolism*; D000067816:Whey Proteins; D000094122:Polyhydroxybutyrates
D003470:Culture Media; D006885:Hydroxybutyrates; D008894:Milk Proteins; D009584:Nitrogen; D011091:Polyesters; D013381:Subtilisins; D000067816:Whey Proteins; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates