In vitro propagation of Coleus forskohlii Briq., a threatened medicinal plant.
In vitro clonai multip1ication of Coleus forskahlii Briq a threatened plant, has been achieved on MS medium supplemented with Kn (2.0 mg/l) and IAA (1.0 mg/l) using nodal segments as explants, Shoots multiplied at a rate of 12 - fold every six weeks. Rooting was achieved upon transfer of shoots onto MS medium containing IAA (1.0 mg/l). The micropropagated plants were successfully established under field conditions. Forskolin content in tubers of plants obtained by micropropagation was found to be 0.1%, the same as that found in wild plants. This micropropagation procedure should be useful for conservation as well as production of this important plant.
National Plant Tissue Culture Repository, National Bureau of Plant Genetic Resources, Pusa Campus, 110012, New Delhi, India.
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N
Sharma
K P
KP
Chandel
V K
VK
Srivastava