Comparative expression analysis of embryonic development-related genes at different stages of parthenogenetic and in vitro fertilized embryos in caprine.
Aberrant gene expression occurs in parthenogenetic embryos due to abnormal epigenetic modifications in the genome that probably diminish viability and enhance developmental abnormalities in these embryos. In the present study, five developmentally important genes (HPRT1, Cx43, Sox2, Mest and IGF2R) were analysed at different stages in parthenotes (haploid and diploid) and compared with similar stages in in vitro fertilized (IVF) embryos. The results indicated that in haploid parthenotes expression of HPRT1 was upregulated (P < 0.05) only at the 2-4-cell stage whereas Cx43 expression was significantly (P < 0.05) downregulated in all stages as compared with the control. However, expression of this gene was upregulated (P < 0.05) in 2-4-cell and morula stages of diploid parthenotes. Expression of Sox2 was significantly (P < 0.05) downregulated in morula stage haploid parthenotes, whereas it was upregulated (P < 0.05) in 8-16-cell stage diploid embryos. The expression of Mest was upregulated (P < 0.05) at the 2-4-cell stage of both haploid and diploid parthenotes, whereas it was downregulated in 8-16-cell stage diploid embryos as compared with control. IGF2R expression was upregulated (P < 0.05) only in morula stage haploid and diploid parthenote as compared with control. These results indicate that parthenogenetic embryos showed aberrant gene expression of developmentally important genes such as HPRT1, Cx43, Sox2, Mest and IGF2R in comparison with IVF embryos, this finding may be one of the major reasons for the poor developmental competence of parthenogenetic embryos.
Indian Veterinary Research Institute,Izatnagar,Bareilly,Uttar Pradesh,India243 122.
Renu
R
Singh
Indian Veterinary Research Institute,Izatnagar,Bareilly,Uttar Pradesh,India243 122.
Kuldeep
K
Kumar
Indian Veterinary Research Institute,Izatnagar,Bareilly,Uttar Pradesh,India243 122.
R
R
Ranjan
Indian Veterinary Research Institute,Izatnagar,Bareilly,Uttar Pradesh,India243 122.
Manish
M
Kumar
Indian Veterinary Research Institute,Izatnagar,Bareilly,Uttar Pradesh,India243 122.
T
T
Yasotha
Indian Veterinary Research Institute,Izatnagar,Bareilly,Uttar Pradesh,India243 122.
R K
RK
Singh
Indian Veterinary Research Institute,Izatnagar,Bareilly,Uttar Pradesh,India243 122.
B C
BC
Das
Indian Veterinary Research Institute,Izatnagar,Bareilly,Uttar Pradesh,India243 122.
M
M
Sarkar
Physiology and Climatology Division,Indian Veterinary Research Institute,Izatnagar-243 122,Bareilly,India.
Sadhan
S
Bag
D000818:Animals; D018031:Connexin 43 / Q000235:genetics; D004171:Diploidy; D004622:Embryo, Mammalian; D005260:Female; D005307:Fertilization in Vitro; D018507:Gene Expression Regulation, Developmental; D006041:Goats / Q000196:embryology* / Q000235:genetics*; D006238:Haploidy; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D059471:In Vitro Oocyte Maturation Techniques; D010312:Parthenogenesis / Q000235:genetics*; D017527:Receptor, IGF Type 2 / Q000235:genetics; D055748:SOXB1 Transcription Factors / Q000235:genetics
D018031:Connexin 43; D017527:Receptor, IGF Type 2; D055748:SOXB1 Transcription Factors; D007041:Hypoxanthine Phosphoribosyltransferase
Parthenogenetic embryos