Effects of low intensity static magnetic field on FTIR spectra and ROS production in SH-SY5Y neuronal-like cells.
Biological effects of man-made electromagnetic fields (EMFs) have been studied so far by experimental approaches exposing animals and cell cultures to EMFs. However, the evidence for cell toxicity induced by static magnetic field (SMF) is still uncertain. We investigated the effects produced by the exposure of human SH-SY5Y neuronal-like cells to a uniform magnetic field at intensities of 2.2 mT, which is less than the recommended public exposure limits set by the International Commission on Non-Ionizing Radiation Protection (ICNIRP). A decrease of membrane mitochondrial potential up to 30% was measured after 24 h of exposure to SMF in SH-SY5Y cells, and this effect was associated with reactive oxygen species production increase. Fourier transform infrared spectroscopy (FTIR) analysis showed that exposure to a static magnetic intensity around 2.2 mT changed the secondary structure of cellular proteins and lipid components. The vibration bands relative to the methylene group increased significantly after 4 h of exposure, whereas further exposure up to 24 h produced evident shifts of amide I and II modes and a relative increase in β-sheet contents with respect to α-helix components. Our study demonstrated that a moderate SMF causes alteration in cell homeostasis, as indicated by FTIR spectroscopy observations of changes in protein structures that are part of cell response to magnetic field exposure.
Department of Physics, University of Messina, Messina, Italy.
Emanuele
E
Calabrò
Salvatore
S
Condello
Monica
M
Currò
Nadia
N
Ferlazzo
Daniela
D
Caccamo
Salvatore
S
Magazù
Riccardo
R
Ientile
D045744:Cell Line, Tumor; D002470:Cell Survival; D006801:Humans; D060526:Magnetic Fields / Q000009:adverse effects*; D053078:Membrane Potential, Mitochondrial; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors
D017382:Reactive Oxygen Species
Fourier transform infrared spectroscopy (FTIR); SH-SY5Y neuronal-like cells; mitochondrial transmembrane potential; static magnetic field