Role of histamine in altering fluid recycling in normal and post-traumatic rabbit peritoneum.
This study aims to investigate if histamine induces electrochemical alterations in the normal and post-traumatic peritoneum. Peritoneal rabbit specimens were obtained before surgery and 10 days post-operatively and were mounted in Ussing chambers. Histamine solutions were added facing the intra-peritoneal and outer-peritoneal surface. Dimetindene maleate-, cetirizine-, and ranitidine-pretreated specimens were used to investigate histamine receptor involvement, whereas amiloride- and ouabain-pretreated specimens were used to investigate ion transportation blockage involvement. Trans-mesothelial resistance (R(TM)) was determined. Histamine-increased R(TM) intra-peritoneally and decreased it outer-peritoneally. A less intense effect was induced in post-traumatic specimens. Dimetindene maleate, cetirizine, amiloride, and ouabain totally inhibited this effect, whereas ranitidine only had a partial effect. Histamine induces electrochemical alterations in the normal and post-operative peritoneum. This effect is mediated by interaction with histamine receptors, hindering the normal process of ion trans-mesothelial transportation.
Department of Physiology, Medical School, University of Thessaly, Larissa, Greece, kouritas@otenet.gr.
Vasileios K
VK
Kouritas
Konstantinos
K
Tepetes
Michalis
M
Spyridakis
Konstantina V
KV
Theodosopoulou
Konstantinos I
KI
Gourgoulianis
Paschalis A
PA
Molyvdas
Chrisi
C
Hatzoglou
D000818:Animals; D004305:Dose-Response Relationship, Drug; D017097:Electric Impedance; D005260:Female; D018495:Fluid Shifts / Q000187:drug effects*; D006632:Histamine / Q000494:pharmacology*; D006633:Histamine Antagonists / Q000494:pharmacology; D017136:Ion Transport; D010537:Peritoneum / Q000187:drug effects* / Q000378:metabolism / Q000601:surgery; D010539:Permeability; D011817:Rabbits; D011968:Receptors, Histamine / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
D006633:Histamine Antagonists; D011968:Receptors, Histamine; D006632:Histamine