Quantification of ¹³C enrichments and isotopomer abundances for metabolic flux analysis using 1D NMR spectroscopy.
The analysis of stable isotope incorporation following feeding of (13)C-labeled precursors to plant tissues provides the constraints necessary for metabolic flux analysis. This protocol describes the use of one-dimensional (1)H and (13)C nuclear magnetic resonance spectroscopy for the quantification of (13)C enrichments and isotopomer abundances in mixtures of metabolites or hydrolyzed biomass components.
Department of Plant Sciences, University of Oxford, Oxford, UK.
Shyam K
SK
Masakapalli
R George
RG
Ratcliffe
Thomas C R
TC
Williams
D002247:Carbon Isotopes; D005583:Fourier Analysis; D009682:Magnetic Resonance Spectroscopy; D064688:Metabolic Flux Analysis; D010944:Plants / Q000378:metabolism*; D013194:Staining and Labeling
D002247:Carbon Isotopes