Delivery of basic fibroblast growth factors from heparinized decellularized adipose tissue stimulates potent de novo adipogenesis.
Scaffolds based on decellularized adipose tissue (DAT) are gaining popularity in adipose tissue engineering due to their high biocompatibility and adipogenic inductive property. However, previous studies involving DAT-derived scaffolds have not fully revealed their potentials for in vivo adipose tissue construction. With the aim of developing a more efficient adipose tissue engineering technique based on DAT, in this study, we investigated the in vivo adipogenic potential of a basic fibroblast growth factor (bFGF) delivery system based on heparinized DAT (Hep-DAT). To generate this system, heparins were cross-linked to mouse DATs by using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide and N-Hydroxysuccinimide. The bFGF-binding Hep-DATs were first tested for controlled release ability in vitro and then transplanted subcutaneously. Highly vascularized adipose tissues were formed 6weeks after transplantation. Histology and gene expression analysis revealed that majority of the Hep-DAT scaffolds were infiltrated with host-derived adipose tissues that possessed similar adipogenic and inflammatory gene expression as endogenous adipose tissues. Additionally, strong de novo adipogenesis could also be induced when bFGF-binding Hep-DATs were thoroughly minced and injected subcutaneously. In conclusion, our study demonstrated that bFGF-binding Hep-DAT could be an efficient, biocompatible and injectable adipogenic system for in vivo adipose tissue engineering.
Faculty of Dentistry, National University of Singapore, Singapore, Singapore; NUS Graduate School for Integrative Science and Engineering, National University of Singapore, Singapore, Singapore.
Qiqi
Q
Lu
Faculty of Dentistry, National University of Singapore, Singapore, Singapore.
Mingming
M
Li
Faculty of Dentistry, National University of Singapore, Singapore, Singapore.
Yu
Y
Zou
Faculty of Dentistry, National University of Singapore, Singapore, Singapore; NUS Graduate School for Integrative Science and Engineering, National University of Singapore, Singapore, Singapore. Electronic address: dencaot@nus.edu.sg.
Tong
T
Cao
D050156:Adipogenesis; D000273:Adipose Tissue / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000378:metabolism; D020869:Gene Expression Profiling; D006493:Heparin / Q000737:chemistry / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018919:Neovascularization, Physiologic; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
D016222:Fibroblast Growth Factor 2; D006493:Heparin
Adipose tissue engineering; ECM; Growth factors; Heparin