Factors affecting the use of chloramphenicol acetyltransferase as a marker for Brassica genetic transformation.
The CAT gene which codes for the enzyme chloramphenicol acetyltransferase was found to be ineffective as a reporter gene in cells and tissues of Brassica species. High levels of endogenous CAT activity were found to be widespread among this genus and did not appear to be distributed in a tissue- or cell-specific manner. Moreover, the presence of an inhibitor of CAT activity was discovered in Brassica napus and Brassica juncea. This inhibitor appeared to act selectively on bacterial CAT in transgenic plants. These findings provided an explanation for difficulties experienced in the detection of transgenic CAT activity in B. napus.
Department of Biology, Carleton University, Ottawa, Ontario, Canada, K1S 5B6, Canada.
P J
PJ
Charest
V N
VN
Iyer
B L
BL
Miki