[Effect of meliae toosendan fructus on nerves system and its mechanism].
OBJECTIVE
To study the effect of the ethanol extract of stir-bake to yellowish Meliae Toosendan Fructus on nerve system and its mechanism.


METHODS
The effect of the ethanol extract on sensory nerve was carried out through ache models induced by hot board method and radiant heat stimulation method in mice. The thermalgesia liminal value was investigated. The effect of the ethanol extract on the A-delta fiber and C fiber was measured by electrical stimulation procedure. Motor nerve conduction velocity (NCV) was measured by indirect detection method in vivo. The pathology changes of the motor nerve were observed by transmission electron microscope and the silver stain test.


RESULTS
The ethanol extract of Meliae Toosendan Fructus could increase the thermalgesia liminal value of mice and reduce the conduction velocity of motor nerves. Meanwhile, pathology results showed the changes of the fiber of motor nerve, including demyelination and the number of Schwann cells dropping.


CONCLUSIONS
The ethanol extract of stir-bake to yellowish Meliae Toosendan Fructus can reduce the pain sensitivity of mice and slow down NCV, which may be related to decreasing of the number of Schwann cells.
Sichuan Academy of Chinese Medicine Sciences, Chengdu 610041, China.
Xiao-Xue
XX
Xiang
Da-Xuan
DX
Tang
Jing-Yue
JY
Xiong
Ya-Jun
YJ
Liang
Dao-Hua
DH
Mu
Xiao-Wei
XW
Yang
Min
M
Hang
Zheng-Huai
ZH
Tan
D000818:Animals; D002452:Cell Count; D005260:Female; D005638:Fruit / Q000737:chemistry; D008297:Male; D029582:Meliaceae / Q000737:chemistry*; D051379:Mice; D009046:Motor Neurons / Q000502:physiology; D036421:Nerve Fibers, Unmyelinated / Q000502:physiology; D009431:Neural Conduction / Q000187:drug effects* / Q000502:physiology; D010146:Pain / Q000503:physiopathology*; D010147:Pain Measurement; D017288:Pain Threshold / Q000187:drug effects*; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000502:physiology; D012584:Sciatic Nerve / Q000187:drug effects / Q000503:physiopathology*
D010936:Plant Extracts