PCR-restriction fragment length polymorphism analysis using groEL gene to differentiate pathogenic Vibrio species.
Important pathogenic Vibrio species were differentiated by PCR-restriction fragment length polymorphism analysis. A 1117-bp groEL gene product was amplified using universal primers and digested using the restriction enzymes NruI or XbaI, revealing unique digestion patterns for each of the 10 Vibrio species, of which 7 were pathogenic in humans, along with 2 other species pathogenic in fish.
Department of Biotechnology, Pukyong National University, Busan, 608-737 Korea.
Muhammad Tofazzal
MT
Hossain
Department of Biotechnology, Pukyong National University, Busan, 608-737 Korea.
Yu-Ri
YR
Kim
Department of Biotechnology, Pukyong National University, Busan, 608-737 Korea. Electronic address: iskong@pknu.ac.kr.
In-Soo
IS
Kong
D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D018834:Chaperonin 60 / Q000235:genetics*; D004269:DNA, Bacterial / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005399:Fishes; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D014733:Vibrio / Q000145:classification* / Q000235:genetics*
D018834:Chaperonin 60; D004269:DNA, Bacterial; C055654:endodeoxyribonuclease NruI; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific
PCR-RFLP; Pathogenic Vibrio species; Species-specific identification; groEL gene