Single step BP/LR combined Gateway reactions.
The Gateway recombination system is characterized by its ability to transfer DNA sequences back and forth between an intermediate clone (the entry clone) and a variety of destination vectors. However, a number of applications do not need to exploit the advantages offered by the entry clone. Here we report reaction conditions for cloning DNA fragments into destination vectors in a single step reaction, thus reducing the cost and overall time needed to obtain an expression clone from three days to one.
Life Technologies, Carlsbad, CA.
Xiquan
X
Liang
Lansha
L
Peng
Chang-Ho
CH
Baek
Federico
F
Katzen
D010582:Bacteriophage lambda / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic
D004247:DNA