Adherens junction distribution mechanisms during cell-cell contact elongation in Drosophila.
During Drosophila gastrulation, amnioserosa (AS) cells flatten and spread as an epithelial sheet. We used AS morphogenesis as a model to investigate how adherens junctions (AJs) distribute along elongating cell-cell contacts in vivo. As the contacts elongated, total AJ protein levels increased along their length. However, genetically blocking this AJ addition indicated that it was not essential for maintaining AJ continuity. Implicating other remodeling mechanisms, AJ photobleaching revealed non-directional lateral mobility of AJs along the elongating contacts, as well as local AJ removal from the membranes. Actin stabilization with jasplakinolide reduced AJ redistribution, and live imaging of myosin II along elongating contacts revealed fragmented, expanding and contracting actomyosin networks, suggesting a mechanism for lateral AJ mobility. Actin stabilization also increased total AJ levels, suggesting an inhibition of AJ removal. Implicating AJ removal by endocytosis, clathrin endocytic machinery accumulated at AJs. However, dynamin disruption had no apparent effect on AJs, suggesting the involvement of redundant or dynamin-independent mechanisms. Overall, we propose that new synthesis, lateral diffusion, and endocytosis play overlapping roles to populate elongating cell-cell contacts with evenly distributed AJs in this in vivo system.
Department of Cell & Systems Biology, University of Toronto, Toronto, Ontario, Canada.
Gabrielle
G
Goldenberg
Tony J C
TJ
Harris
D000205:Actomyosin / Q000378:metabolism; D022005:Adherens Junctions; D000818:Animals; D002450:Cell Communication; D048430:Cell Shape; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D034281:Dynamins / Q000378:metabolism; D004705:Endocytosis; D005089:Exocytosis; D054262:Gastrulation; D009068:Movement
D029721:Drosophila Proteins; D000205:Actomyosin; D034281:Dynamins