ATP transport through VDAC and the VDAC-tubulin complex probed by equilibrium and nonequilibrium MD simulations.
Voltage-dependent anion channel (VDAC), the major channel of the mitochondrial outer membrane, serves as a principal pathway for ATP, ADP, and other respiratory substrates across this membrane. Using umbrella-sampling simulations, we established the thermodynamic and kinetic components governing ATP transport across the VDAC1 channel. We found that there are several low-affinity binding sites for ATP along the translocation pathway and that the main barrier for ATP transport is located around the center of the channel and is formed predominantly by residues in the N-terminus. The binding affinity of ATP to an open channel was found to be in the millimolar to micromolar range. However, we show that this weak binding increases the ATP translocation probability by about 10-fold compared with the VDAC pore in which attractive interactions were artificially removed. Recently, it was found that free dimeric tubulin induces a highly efficient, reversible blockage of VDAC reconstituted into planar lipid membranes. It was proposed that by blocking VDAC permeability for ATP/ADP and other mitochondrial respiratory substrates tubulin controls mitochondrial respiration. Using the Rosetta protein-protein docking algorithm, we established a tentative structure of the VDAC-tubulin complex. An extensive set of equilibrium and nonequilibrium (under applied electric field) molecular dynamics (MD) simulations was used to establish the conductance of the open and blocked channel. It was found that the presence of the unstructured C-terminal tail of tubulin in the VDAC pore decreases its conductance by more than 40% and switches its selectivity from anionic to cationic. The subsequent 1D potential of mean force (PMF) computations for the VDAC-tubulin complex show that the state renders ATP transport virtually impossible. A number of residues pivotal for tubulin binding to the channel were identified that help to clarify the molecular details of VDAC-tubulin interaction and to provide new insight into the mechanism of the control of mitochondria respiration by VDAC.
Center for Molecular Simulations, Department of Biological Sciences, University of Calgary , Calgary, Alberta T2N 1N4, Canada.
Sergei Yu
SY
Noskov
Tatiana K
TK
Rostovtseva
Sergey M
SM
Bezrukov
D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D001692:Biological Transport; D002417:Cattle; D030562:Databases, Protein; D015536:Down-Regulation; D007700:Kinetics; D053078:Membrane Potential, Mitochondrial; D051379:Mice; D051336:Mitochondrial Membranes / Q000378:metabolism*; D008958:Models, Molecular; D008968:Molecular Conformation; D062105:Molecular Docking Simulation; D056004:Molecular Dynamics Simulation; D054730:Protein Interaction Domains and Motifs; D020836:Protein Structure, Quaternary; D058767:Protein Unfolding; D014404:Tubulin / Q000737:chemistry / Q000378:metabolism*; D050995:Voltage-Dependent Anion Channel 1 / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*
D014404:Tubulin; C494763:Vdac1 protein, mouse; D000255:Adenosine Triphosphate; D050995:Voltage-Dependent Anion Channel 1