The importance of Arabidopsis glutathione peroxidase 8 for protecting Arabidopsis plant and E. coli cells against oxidative stress.
Glutathione peroxidases (GPXs) are major family of the reactive oxygen species (ROS) scavenging enzymes. Recently, database analysis of the Arabidopsis genome revealed a new open-reading frame, thus increasing the total number of AtGPX gene family to eight (AtGPX1-8). The effect of plant hormones like; i. e. salicylic acid (SA), jasmonic acid (JA), abscisic acid (ABA), indoleacetic acid (IAA), and mannitol on the expression of the genes confirm that the AtGPX genes family is regulated by multiple signaling pathways. The survival rate of AtGPX8 knockout plants (KO8) was significantly decreased under heat stress compared with the wild type. Moreover, the content of malondialdehyde (MDA) and protein oxidation was significantly increased in the KO8 plant cells under heat stress. Results indicating that the deficiency of AtGPX8 accelerates the progression of oxidative stress in KO8 plants. On the other hand, the overexpression of AtGPX8 in E. coli cells enhance the growth of the recombinant enzyme on media supplemented with 0.2 mM cumene hydroperoxide, 0.3 mM H 2O 2 or 600 mM NaCl.
Department of Genetics; Faculty of Agriculture, Cairo University; Giza, Egypt.
Ahmed
A
Gaber
D000222:Adaptation, Physiological / Q000187:drug effects; D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001555:Benzene Derivatives / Q000494:pharmacology; D015153:Blotting, Western; D019610:Cytoprotection / Q000187:drug effects; D004926:Escherichia coli / Q000166:cytology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000494:pharmacology; D005810:Multigene Family; D009154:Mutation / Q000235:genetics; D018384:Oxidative Stress / Q000187:drug effects; D010641:Phenotype; D059828:Plant Cells / Q000187:drug effects / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D054712:Salinity; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics
D029681:Arabidopsis Proteins; D001555:Benzene Derivatives; D010937:Plant Growth Regulators; D012333:RNA, Messenger; D011994:Recombinant Proteins; D012965:Sodium Chloride; D006861:Hydrogen Peroxide; C581963:GPX8 protein, Arabidopsis; D005979:Glutathione Peroxidase; C007164:cumene hydroperoxide
ArabidopsisATGPX8; E. coli; heat stress; oxidative stress; recombinant enzyme