[Optimization for preparation technology of shangke jiefu lotion by orthogonal test].
OBJECTIVE
To optimize the preparation technology of Shangke Jiefu lotion.


METHODS
The extraction process was optimized by orthogonal test, with water addition, extraction times and time used for extraction as factors of investigation. In refined process test, alcohol precipitation concentration, time, and the relative density of extract were studied. Each factor had three levels. The content of sophorcarpidine and the yield of dry extract were used as the evaluation indexes. The content of sophorcarpidine was determined by HPLC, and dry extract rates were determined by drying method.


RESULTS
The best extraction condition was as follows: the amount of water was 10 times of the medicinal materials, the decoction duration was 2 h and for 3 times. The optimum purification process was: alcohol precipitation concentration was 50%, time was 15 hours, relative density of extract was 1.05 g/mL.


CONCLUSIONS
The optimized preparation technology of Shangke Jiefu lotion is stable, feasible and convenient. It provides a theoretical basis for standardized production.
Gansu Hospital of TCM, Lanzhou 730050, China. liuxiaoshuan1964@163.com
Xiao-Shuan
XS
Liu
Er-Lin
EL
Chen
Xiao-Hua
XH
Zhang
Ji-Wen
JW
Li
Yan-Kui
YK
Li
Xi-Xiang
XX
Li
D000470:Alkaloids / Q000032:analysis*; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004202:Disinfectants / Q000737:chemistry / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D006358:Hot Temperature; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D011807:Quinolizines / Q000032:analysis*; D029909:Sophora / Q000737:chemistry; D013997:Time Factors; D014867:Water / Q000737:chemistry; D000093842:Matrines
D000470:Alkaloids; D004202:Disinfectants; D004365:Drugs, Chinese Herbal; D011807:Quinolizines; D014867:Water; D000093842:Matrines