Isolation and characterization of a novel native Bacillus thuringiensis strain BRC-HZM2 capable of degrading chlorpyrifos.
Studies were carried out to isolate chlorpyrifos degrading Bacillus thuringiensis (Bt) strains from chlorpyrifos-contaminated samples. Six Bt strains (isolation rate 2.7%) were isolated by modified sodium acetate antibiotic heat treatment, and one novel strain (BRC-HZM2) was selected for further analysis. Phenotype and phylogeny analysis of this strain was conducted on the basis of biochemical reactions, antibiotic sensitivity, 16s rRNA genes, plasmid profile, insecticidal crystal protein profiles, and PCR-RFLP for cry and cyt genes. The degradation rate of chlorpyrifos in liquid culture was estimated during 48 h of incubation for the isolate BRC-HZM2. More than 50% of the initial chlorpyrifos concentration degraded within 12 h, 88.9% after 48 h. These results highlight the potential of the Bt strain for biological control and the bioremediation of environments contaminated with chlorpyrifos.
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, P. R. China.
Songqing
S
Wu
Yan
Y
Peng
Zhangmin
Z
Huang
Zhipeng
Z
Huang
Lei
L
Xu
Gelbič
G
Ivan
Xiong
X
Guan
Lingling
L
Zhang
Shuangquan
S
Zou
D001413:Bacillus thuringiensis / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002681:China; D004390:Chlorpyrifos / Q000032:analysis / Q000378:metabolism*; D007306:Insecticides / Q000378:metabolism; D010641:Phenotype; D010802:Phylogeny; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D007306:Insecticides; D012336:RNA, Ribosomal, 16S; D004390:Chlorpyrifos
Bacillus thuringiensis; Biodegradation; Biopesticide; Chlorpyrifos; Identification