[Optimization of the extraction process of the effective components in fructus phyllanthi].
OBJECTIVE
To optimize the extraction process of the effective components in Fructus Phyllanthi.


METHODS
Using Fructus Phyllanthi from Puning, Guangdong as the raw materials, its extraction process was screened by the orthogonal design. The extraction rates of polyphenol from Fructus Phyllanthi under different extraction conditions were compared to determine the optimum extraction technology. The effects of the ratio of raw materials to liquid, extraction time, extraction temperature and extraction number on extraction rates of polysaccharose in Fructus Phyllanthi were also investigated.


RESULTS
The optimum extraction conditions for polyphenol were as follows: 50% methanol as solvent, ratio of raw materials to liquid 1:10, extraction times 1, extraction time 90 min. The optimum extracting conditions for polysaccharose were as follows: ratio of raw materials to liquid 1:20, extraction time 3.0 h, extraction times 3, and extraction temperature 90 degrees C.


CONCLUSIONS
The optimization of the extraction process for polyphenol and polysaccharide in Fructus Phyllanthi has a good reference guide for further developing a seris of health care products of Fructus Phyllanthi.
Drug Research and Development Center of Sun Yat-sen University, Guangzhou 510080, China.
Wei
W
Cao
Jian-Mei
JM
Zhu
Jie
J
Wen
Li-Ping
LP
Yu
D000704:Analysis of Variance; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D029842:Phyllanthus emblica / Q000737:chemistry*; D059808:Polyphenols / Q000032:analysis / Q000302:isolation & purification*; D011134:Polysaccharides / Q000032:analysis / Q000302:isolation & purification*; D011786:Quality Control; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
D059808:Polyphenols; D011134:Polysaccharides; D012997:Solvents; D000432:Methanol