Different levels of HIV DNA copy numbers in cerebrospinal fluid cellular subsets.
Inequities in the incidence of HIV infection and AIDS with continued persistence of HIV-associated neurocognitive disorders (HAND) exist in populations in Hawaii (HI) and Puerto Rico (PR). We previously reported that peripheral monocyte HIV DNA levels are high in patients in Hawaii with HAND and we now hypothesize that similar findings would be observed in the cerebrospinal fluid (CSF) cellular subsets. Cerebrospinal fluid cells were obtained from patients from PR and HI undergoing neurocognitive testing and sorted into monocytes (CD14+) and lymphocytes (CD14-) and HIV DNA was measured. From six PR subjects (three HAND, three normal cognition, NC) and six HI subjects (three HAND, three NC), HIV DNA burden in CD14+ cells was higher in HAND than NC patients; NC patients had higher HIV DNA burden in CD14-cells versus HAND. Differences in HIV DNA burden in particular CSF cellular subsets suggest that HIV DNA burden may play a role in HAND neuropathogenesis.
Melissa
M
Agsalda-Garcia
Bruce
B
Shiramizu
Loyda
L
Melendez
Marines
M
Plaud
Chin-Yuan
CY
Liang
Valerie
V
Wojna
D000328:Adult; D003072:Cognition Disorders / Q000209:etiology*; D015331:Cohort Studies; D004279:DNA, Viral / Q000134:cerebrospinal fluid*; D005260:Female; D015658:HIV Infections / Q000134:cerebrospinal fluid* / Q000150:complications; D006254:Hawaii; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism; D011647:Puerto Rico; D012494:Sampling Studies
D004279:DNA, Viral; D018950:Lipopolysaccharide Receptors