Use of electroporation and conjugative mobilization for genetic manipulation of Staphylococcus epidermidis.
To perform mechanistic studies on the biology of bacteria including metabolism, physiology, and pathogenesis, it is essential to possess the tools required for genetic manipulation. Introduction of plasmid DNA into Staphylococcus epidermidis for subsequent genetic manipulation, including allelic replacement and complementation experiments, is typically performed by either electroporation or conjugative mobilization. Herein, standard protocols and tips for the transfer of plasmid DNA to S. epidermidis by these two methods are provided.
Department of Pathology and Microbiology, Center for Staphylococcal Research, University of Nebraska Medical Center, Omaha, NE, USA.
Katherine L
KL
Maliszewski
Austin S
AS
Nuxoll
D018274:Electroporation; D005818:Genetic Engineering; D010957:Plasmids / Q000235:genetics; D013212:Staphylococcus epidermidis / Q000235:genetics*; D014162:Transfection / Q000379:methods*; D014169:Transformation, Bacterial