Regulation of Cu-Zn superoxide dismutase on SCN2A in SH-SY5Y cells as a potential therapy for temporal lobe epilepsy.
In order to evaluate SCN2A as a candidate gene for epileptic susceptibility and the use of a Cu-Zn superoxide dismutase (SOD) supplement as a potential therapy for epilepsy, SCN2A expression in the cortex and the correlation between SCN2A and Cu-Zn SOD in SH-SY5Y cells were examined. SCN2A expression and the concentration of Cu-Zn SOD in the cerebral cortexes of patients with primary and secondary temporal lobe epilepsy and normal brain cortex tissues were detected. By transfecting SH-SY5Y cells, the expression of SCN2A and the concentration of Cu-Zn SOD was analyzed and the single-cell patch clamp technique was employed in order to investigate the changes in sodium ion levels following SCN2A knockdown. SCN2A level restoration was also investigated with a Cu-Zn SOD supplement using an expression study and evaluated the changes in sodium ion levels following SCN2A knockdown. SCN2A expression and Cu-Zn SOD concentration decreased in the epileptic cerebral cortex. Following SCN2A knockdown, the concentration of Cu-Zn SOD declined and the si-SCN2A vector group showed a repeated discharge. Furthermore, the Cu-Zn SOD concentration was capable of restoring the expression of SCN2A following SCN2A knockdown in SH-SY5Y cells and the overexpression of Cu-Zn SOD prevented the repeated discharge caused by si-SCN2A. The results indicated that there is a low expression of SCN2A and Cu-Zn SOD in the epileptic cerebral cortex and provided novel insights into potential therapies for temporal lobe epilepsy.
Department of Neurosurgery, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.
Jun
J
Xiang
Yugang
Y
Jiang
D000328:Adult; D045744:Cell Line, Tumor; D002540:Cerebral Cortex / Q000378:metabolism; D015536:Down-Regulation; D004833:Epilepsy, Temporal Lobe / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology*; D005822:Genetic Vectors / Q000378:metabolism; D006801:Humans; D007477:Ions / Q000737:chemistry / Q000378:metabolism; D008875:Middle Aged; D062551:NAV1.2 Voltage-Gated Sodium Channel / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D012964:Sodium / Q000737:chemistry / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism*; D014162:Transfection
D007477:Ions; D062551:NAV1.2 Voltage-Gated Sodium Channel; D012333:RNA, Messenger; C568251:SCN2A protein, human; D012964:Sodium; D013482:Superoxide Dismutase