Clinical and serological findings associated with the expression of ITGAL, PRF1, and CD70 in systemic lupus erythematosus.
We determined the expression of Integrin alpha L chain (ITGAL), Perforin 1 (PRF1), and CD70 and studied the associations with laboratory and clinical parameters. CD4+ T cells were isolated from 35 SLE patients and 30 healthy controls. The transcript levels of ITGAL, PRF1, and CD70 were quantified by real-time reverse-transcription polymerase chain reaction (RT-PCR). The SLE patients had significantly elevated transcript levels of ITGAL (18.61±22.17 vs. 7.33±9.17, p=0.042), PRF1 (21.67±26.34 vs. 10.67±11.65, p=0.039), and CD70 (1.45±1.63 vs. 0.67± 0.28, p=0.011). Patients with anti-microsomal and/or anti-thyroglobulin antibodies showed high levels of ITGAL (33.41±30.14 vs. 13.58±16.43, p=0.044; and 34.01±27.66 vs. 11.90±16.17, p=0.007, respectively). No association was seen either for the typical antibodies of SLE or for the disease activity. Although ITGAL, PRF1, and CD70 are overexpressed in SLE CD4+ T cells, their expression is not linked to the typical clinical and serological parameters associated with the disease. The role that ITGAL may play in autoimmune thyroiditis deserves further investigation.
Research Unit in Systemic Autoimmune Diseases, Vall d'Hebron Research Institute, Universitat Autonoma de Barcelona, Barcelona, Spain. ebalada@ir.vhebron.net.
Eva
E
Balada
Jesus
J
Castro-Marrero
Lledo
L
Felip
Josep
J
Ordi-Ros
Miquel
M
Vilardell-Tarrés
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D040881:CD11a Antigen / Q000235:genetics*; D053282:CD27 Ligand / Q000235:genetics*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D016022:Case-Control Studies; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins / Q000235:genetics*; D011237:Predictive Value of Tests; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012698:Serologic Tests; D015854:Up-Regulation; D055815:Young Adult
D015415:Biomarkers; D040881:CD11a Antigen; D053282:CD27 Ligand; C505771:CD70 protein, human; C510468:PRF1 protein, human; D052899:Pore Forming Cytotoxic Proteins; D012333:RNA, Messenger; D054353:Perforin