Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum).
Pectinolytic bacteria have been recently isolated from diseased potato plants exhibiting blackleg and slow wilt symptoms found in a number of European countries and Israel. These Gram-reaction-negative, motile, rods were identified as belonging to the genus Dickeya, previously the Pectobacterium chrysanthemi complex (Erwinia chrysanthemi), on the basis of production of a PCR product with the pelADE primers, 16S rRNA gene sequence analysis, fatty acid methyl esterase analysis, the production of phosphatases and the ability to produce indole and acids from α-methylglucoside. Differential physiological assays used previously to differentiate between strains of E. chrysanthemi, showed that these isolates belonged to biovar 3. Eight of the isolates, seven from potato and one from hyacinth, were analysed together with 21 reference strains representing all currently recognized taxa within the genus Dickeya. The novel isolates formed a distinct genetic clade in multilocus sequence analysis (MLSA) using concatenated sequences of the intergenic spacer (IGS), as well as dnaX, recA, dnaN, fusA, gapA, purA, rplB, rpoS and gyrA. Characterization by whole-cell MALDI-TOF mass spectrometry, pulsed field gel electrophoresis after digestion of whole-genome DNA with rare-cutting restriction enzymes, average nucleotide identity analysis and DNA-DNA hybridization studies, showed that although related to Dickeya dadantii, these isolates represent a novel species within the genus Dickeya, for which the name Dickeya solani sp. nov. (type strain IPO 2222(T) = LMG25993(T) = NCPPB4479(T)) is proposed.
Plant Research International, P.O. Box 69, 6700 AB Wageningen, The Netherlands.
Jan M
JM
van der Wolf
Plant Research International, P.O. Box 69, 6700 AB Wageningen, The Netherlands.
Els H
EH
Nijhuis
Science and Advice for Scottish Agriculture (SASA), Edinburgh EH12 9FJ, UK.
Malgorzata J
MJ
Kowalewska
Science and Advice for Scottish Agriculture (SASA), Edinburgh EH12 9FJ, UK.
Gerry S
GS
Saddler
Food and Environment Research Agency (FERA), Sand Hutton, York YO41 1LZ, UK.
Neil
N
Parkinson
Food and Environment Research Agency (FERA), Sand Hutton, York YO41 1LZ, UK.
John G
JG
Elphinstone
The James Hutton Institute (JHI), Invergowrie, Dundee DD2 5DA, UK.
Leighton
L
Pritchard
The James Hutton Institute (JHI), Invergowrie, Dundee DD2 5DA, UK.
Ian K
IK
Toth
Intercollegiate Faculty of Biotechnology UG & MUG, Kladki 24, 80-822 Gdansk, Poland.
Ewa
E
Lojkowska
Intercollegiate Faculty of Biotechnology UG & MUG, Kladki 24, 80-822 Gdansk, Poland.
Marta
M
Potrykus
Intercollegiate Faculty of Biotechnology UG & MUG, Kladki 24, 80-822 Gdansk, Poland.
Malgorzata
M
Waleron
BCCM/LMG Bacteria Collection, Ghent University, K.L. Ledeganckstraat 35, B-90000, Ghent, Belgium.
Paul
P
de Vos
BCCM/LMG Bacteria Collection, Ghent University, K.L. Ledeganckstraat 35, B-90000, Ghent, Belgium.
Ilse
I
Cleenwerck
Department of Applied Biology, Plant Pathology, University of Helsinki, 00014 Helsinki, Finland.
Minna
M
Pirhonen
Department of Applied Biology, Plant Pathology, University of Helsinki, 00014 Helsinki, Finland.
Linda
L
Garlant
National Federation of Seed Potato Growers (FN3PT) / INRA UMR 1349 IGEPP, 35653 Le Rheu Cedex, France.
Valérie
V
Hélias
Environmental Genomics and Systems Biology, Institute of Natural Resource Sciences, Zurich University of Applied Sciences, Campus Grüental, P.O. Box CH-8820, Wädenswil, Switzerland.|Agroscope Changins-Wädenswil, Plant Protection Division, ACW, Schloss 1, 8820 Wädenswil, Switzerland.
Joël F
JF
Pothier
Mabritec AG, Lörracherstrasse 50, Postfach 320, 4125 Riehen, Switzerland.
Valentin
V
Pflüger
Environmental Genomics and Systems Biology, Institute of Natural Resource Sciences, Zurich University of Applied Sciences, Campus Grüental, P.O. Box CH-8820, Wädenswil, Switzerland.
Brion
B
Duffy
Agricultural Research Organization, Department of Plant Pathology and Weed Research, Volcani Center, Bet Dagan 50250, Israel.
Leah
L
Tsror
Agricultural Research Organization, Department of Plant Pathology and Weed Research, Volcani Center, Bet Dagan 50250, Israel.
Shula
S
Manulis
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005060:Europe; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D007211:Indoles / Q000378:metabolism; D007557:Israel; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D009693:Nucleic Acid Hybridization; D010368:Pectins / Q000378:metabolism*; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011198:Solanum tuberosum / Q000382:microbiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D004269:DNA, Bacterial; D005227:Fatty Acids; D007211:Indoles; D012336:RNA, Ribosomal, 16S; C030374:indole; D010368:Pectins