[Gene cloning and bioinformatics analysis of SABATH methyltransferase in Lonicera japonica var. chinensis].
OBJECTIVE
To clone SABATH methyltransferase (rLjSABATHMT) gene in Lonicera japonica var. chinensis, and compare the gene expression and intron sequence of SABATH methyltransferase orthologous in L. japonica with L. japonica var. chinensis. It provide a basis for gene regulate the formation of L. japonica floral scents.


METHODS
The cDNA and genome sequences of LjSABATHMT from L. japonica var. chinensis were cloned according to the gene fragments in cDNA library. The LjSABATHMT protein was characterized by bioinformatics analysis. SABATH family phylogenetic tree were built by MEGA 5.0. The transcripted level of SABATHMT orthologous were analyzed in different organs and different flower periods of L. japonica and L. japonica var. chinensis using RT-PCR analysis. Intron sequences of SABATHMT orthologous were also analyzied.


RESULTS
The cDNA of LjSABATHMT was 1 251 bp, had a complete coding frame with 365 amino acids. The protein had the conservative SABATHMT domain, and phylogenetic tree showed that it may be a salicylic acid/benzoic acid methyltransferase. Higher expression of SABATH methyltransferase orthologous was found in flower. The intron sequence of L. japonica and L. japonica var. chinensis had rich polymorphism, and two SNP are unique genotype of L. japonica var. chinensis. The motif elements in two orthologous genes were significant differences.


CONCLUSIONS
The intron difference of SABATH methyltransferase orthologous could be inducing to difference of gene expression between L. japonica and L. japonica var. chinensis. These results will provide important base on regulating active compounds of L. japonica.
Wuhan Polytechnic University, Wuhan 430023, China.
Xiao-Dan
XD
Yu
Chao
C
Jiang
Lu-Qi
LQ
Huang
Shuang-Shuang
SS
Qin
Xiang-Mei
XM
Zeng
Ping
P
Chen
Yuan
Y
Yuan
D001483:Base Sequence; D003001:Cloning, Molecular; D019295:Computational Biology; D019143:Evolution, Molecular; D018506:Gene Expression Regulation, Plant; D031239:Lonicera / Q000201:enzymology* / Q000235:genetics*; D008780:Methyltransferases / Q000235:genetics*; D059372:Nucleotide Motifs; D010802:Phylogeny; D016415:Sequence Alignment; D017421:Sequence Analysis
D008780:Methyltransferases