Immuno-gold localization of α-L-arabinofuranosyl residues in pollen tubes of Nicotiana alata Link et otto.
Immuno-gold labelling using a monoclonal antibody (PCBC3) with a primary specificity for α-L-arabinofuranosyl residues was used to locate these residues in pollen tubes of Nicotiana alata grown in vivo. The antibody bound to the outer fibrillar layer of the pollen-tube wall: the inner, non-fibrillar wall layer was not labelled. Cytoplasmic vesicles (0.2 μm diameter) were also labelled. The antibody may bind to an arabinan in the pollen-tube wall.
Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3052, Parkville, Vic., Australia.
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MA
Anderson
P J
PJ
Harris
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I
Bonig
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Clarke