Aβ-induced microglial cell activation is inhibited by baicalin through the JAK2/STAT3 signaling pathway.
Baicalin has shown multiple neuroprotective biological activities, including antiapoptotic and anti-inflammatory functions in neurodegeneration diseases. However, whether baicalin can regulate Aβ-induced microglial activation or inhibit inflammatory cytokine secretion has not been confirmed. We demonstrated that baicalin can inhibit beta amyloid peptides (Aβ42)-induced BV2 microglial cell proliferation, reduce the expression of CD11b, decrease chemotactic ability of BV2 cells and significantly inhibit the secretion of IL-6, TNF-α and NO. Moreover, baicalin pretreatment can effectively inhibit Aβ-induced phosphorylation of JAK2 and STAT3. Baicalin can inhibit Aβ-induced microglial cell activation by regulating the JAK2/STAT3 signaling pathway in AD transgenic mice. The modulation of microglial proliferation, activation and secretion by baicalin could be a promising therapeutic option for the treatment of Alzheimer's disease.
1Beijing Key Laboratory for Aging and Geriatrics, Institute of Geriatrics, General Hospital of Chinese PLA, Beijing, China.
Jiaxiang
J
Xiong
Changzheng
C
Wang
Hongyan
H
Chen
Yazhuo
Y
Hu
Lei
L
Tian
Jingkun
J
Pan
Miao
M
Geng
D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D053614:Janus Kinase 2 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism; D010446:Peptide Fragments / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011897:Random Allocation; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology
D016229:Amyloid beta-Peptides; D000894:Anti-Inflammatory Agents, Non-Steroidal; D005419:Flavonoids; D010446:Peptide Fragments; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; C075222:amyloid beta-protein (1-42); C038044:baicalin; C507926:Jak2 protein, mouse; D053614:Janus Kinase 2
JAK2/STAT3 pathway; activation; baicalin; inhibition; microglia