Simultaneous determination of 30 ginsenosides in Panax ginseng preparations using ultra performance liquid chromatography.
A quick and simple method for simultaneous determination of the 30 ginsenosides (ginsenoside Ro, Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, 20(S)-Rg2, 20(R)-Rg2, 20(S)-Rg3, 20(R)-Rg3, 20(S)-Rh1, 20(S)-Rh2, 20(R)-Rh2, F1, F2, F4, Ra1, Rg6, Rh4, Rk3, Rg5, Rk1, Rb3, Rk2, Rh3, compound Y, compound K, and notoginsenoside R1) in Panax ginseng preparations was developed and validated by an ultra performance liquid chromatography photo diode array detector. The separation of the 30 ginsenosides was efficiently undertaken on the Acquity BEH C-18 column with gradient elution with phosphoric acids. Especially the chromatogram of the ginsenoside Ro was dramatically enhanced by adding phosphoric acid. Under optimized conditions, the detection limits were 0.4 to 1.7 mg/L and the calibration curves of the peak areas for the 30 ginsenosides were linear over three orders of magnitude with a correlation coefficients greater than 0.999. The accuracy of the method was tested by a recovery measurement of the spiked samples which yielded good results of 89% to 118%. From these overall results, the proposed method may be helpful in the development and quality of P. ginseng preparations because of its wide range of applications due to the simultaneous analysis of many kinds of ginsenosides.
Korea Ginseng Corporation Research Institute, Korea Ginseng Corporation, Daejeon 305-805, Korea.
Hee-Won
HW
Park
Gyo
G
In
Sung-Tai
ST
Han
Myoung-Woo
MW
Lee
So-Young
SY
Kim
Kyung-Tack
KT
Kim
Byung-Goo
BG
Cho
Gyeong-Ho
GH
Han
Il-Moo
IM
Chang
Ginsenosides; Panax ginseng; Panax ginseng preparations; Simultaneous analysis; UPLC-PDA