Assessment of murine neuroblastoma (N1E-115) resting membrane potential by confocal microscopy.
Digital imaging (confocal microscopy) and a slow potentiometric dye (tetramethylrhodamine methyl ester) were used to assess the resting membrane potential (V m) of murine neuroblastoma cells (N1E-115). The averageV m was found to be -64.0±2.0 mV. The difference between this and the previously reported higher values was attributed to the use of glass microelectrode techniques that probably caused mechanical injury to the cell membranes: Digital imaging of N1E-115V m was found to be sensitive, reproducible, fast, and simple.
Biological and Agricultural Engineering Department, Driftmier Engineering Center, University of Georgia, 30602-4435, Athens, Georgia.
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M
Hernandez
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Kisaalita
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Farmer