Partition Affinity Ligand Assay (PALA) : A simple binding assay procedure.
In a competitive binding assay, the ligand to be quantified competes with a fixed amount of labeled ligand for the sites on a limiting amount of binding protein. The amount of label bound is therefore dependent on the ratio between native and labeled ligand. In a binding assay, one must separate the free ligands from bound. The better the separation, the higher the sensitivity of the assay. But effective methods are often laborious and time-consuming and thus we have developed a novel approach, the Partition Affinity Ligand Assay (PALA).
Pure and Applied Biochemistry, Chemical Center, University of Lund, PO Box 740, S-22007, Lund, Sweden.
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Mattiasson