Plant regeneration from stem and petal of carnation (Dianthus caryophyllus L.).
Plants were regenerated via adventitious shoot initiation from petal explants of carnation (Dianthus caryophyllus L.) cultivars Crowley Sim, Ember Rose, Orchid Beauty, Red Sim, White Sim and from stem segments of Crowley Sim, Red Sim, White Sim. Differences in cultivar response were observed, with White Sim being the most responsive for both explant types. Plants were also regenerated from receptacles of this cultivar. The effect of different cytokinins on regeneration from petal and stem explants of cultivar White Sim was compared. Thidiazuron was more effective than 6-benzylaminopurine or kinetin. In stem explants, morphogenic capacity was determined by the developmental stage of the explant. Highest percentage of shoot formation was observed in the youngest stem segments, on all the cytokinins tested. Stem-derived plants grew faster than petal or receptacle-derived plants and produced normal, flowering plants eight to ten months after culture.
Calgene Pacific Pty Ltd, 16 Gipps Street, 3066, Collingwood, Victoria, Australia.
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