Fluorescence-based Broad Dynamic Range Viscosity Probes.
We introduce two new fluorescent viscosity probes, SYBR Green (SG) and PicoGreen (PG), that we have studied over a broad range of viscosity and in collagen solutions. In water, both dyes have low quantum yields and excited state lifetimes, while in viscous solvents or in complex with DNA both parameters dramatically (300-1000-fold) increase. We show that in log-log scale the dependence of the dyes' quantum yield vs. viscosity is linear, the slope of which is sensitive to temperature. Application of SG and PG, as a fluorescence-based broad dynamic range viscosity probes, to the life sciences is discussed.
Institute of Fluorescence and Department of Chemistry and Biochemistry, The Columbus Center, University of Maryland Baltimore County, 701 East Pratt Street, Baltimore, MD, 21202, USA.
Anatoliy
A
Dragan
Institute of Fluorescence and Department of Chemistry and Biochemistry, The Columbus Center, University of Maryland Baltimore County, 701 East Pratt Street, Baltimore, MD, 21202, USA.
August E
AE
Graham
Institute of Fluorescence and Department of Chemistry and Biochemistry, The Columbus Center, University of Maryland Baltimore County, 701 East Pratt Street, Baltimore, MD, 21202, USA. Geddes@umbc.edu.
Chris D
CD
Geddes
D003094:Collagen / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes; D013696:Temperature; D014783:Viscosity
D005456:Fluorescent Dyes; D003094:Collagen
Collagen sensing; Fluorescence probes; Fluorescent viscosity probe; Intramolecular quenching; Molecular rotors; PicoGreen; SYBR Green I