Development of somatic embryos from cell suspension cultures of Norway spruce (Picea abies Karst.).
Cell suspension cultures were established from embryonal-suspensor masses derived from mature seeds. Transfer of cell masses on to a medium containing abscisic acid stimulated development of already established individual embryos. Somatic embryos developed shoots when supported by cheese cloth in liquid medium in Petri dishes. The percentage of well-developed roots remained low even though all embryos had root meristems. We have recovered an average of 25 plantlets from an initial PCV of ca 1 g fresh wt per 10 ml.
Department of Environmental Horticulture, University of California, 95616, Davis, CA, USA.
M P
MP
Boulay
P K
PK
Gupta
P
P
Krogstrup
D J
DJ
Durzan