Immunohistochemical study of pseudohypertrophy of the inferior olivary nucleus.
OBJECTIVE
Pathogenesis of pseudohypertrophy of the inferior olivary nucleus (PH-IO) was analyzed based on immunohistochemical study.


METHODS
Immunostained medullas with PH-IO were observed with confocal laser microscopy.


RESULTS
αB-crystallin (αBC) was frequently expressed in the neurons and co-localized with microtubule-associated protein 2 (MAP2). The neurons were occasionally positive for SMI-31. αBC and SMI-31 were co-localized in some neurons. Synaptophysin (SYP)-immunoreactive dots were present around MAP2-positive hypertrophic neurons and hypertrophic thick neurites. Periphery-stained Lys-Asp-Glu-Leu (KDEL)-positive neurons were shown. Central chromatolytic neurons were found with Klüver-Barrera staining, which indicated that the rough endoplasmic reticulum (ER) was distributed to the periphery of the cytoplasm.


CONCLUSIONS
αBC prevents microtubule disassembly and phosphorylation of the neurofilaments under stressful conditions. Our results indicated that αBC protected microtubules and neurofilaments in PH-IO. The retrograde transport of KDEL receptors from the Golgi complex to the ER is increased under stressful conditions. We considered that KDEL receptors were retro-transported to ER, and then the ER containing KDEL receptors was distributed to the periphery of the cytoplasm. PH-IO showed various immunohistochemical changes due to trans-synaptic degeneration.
Katsuhiko
K
Ogawa
Satoshi
S
Kamei
Kazuaki
K
Ichihara
Kenji
K
Uehara
Yoshio
Y
Suzuki
Toshiki
T
Uchihara
Hirokazu
H
Yoshihashi
Ja-Mun
JM
Chong
D004721:Endoplasmic Reticulum / Q000502:physiology; D006056:Golgi Apparatus / Q000502:physiology; D006801:Humans; D006984:Hypertrophy / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D018613:Microscopy, Confocal; D009847:Olivary Nucleus / Q000378:metabolism* / Q000473:pathology*; D018000:Receptors, Peptide / Q000378:metabolism; D012183:Retrograde Degeneration / Q000150:complications / Q000378:metabolism / Q000473:pathology; D038203:alpha-Crystallin B Chain / Q000378:metabolism
C064459:KDEL receptor; D018000:Receptors, Peptide; D038203:alpha-Crystallin B Chain