Analysis of the Protein phosphotome of Entamoeba histolytica reveals an intricate phosphorylation network.
Phosphorylation is the most common mechanism for the propagation of intracellular signals. Protein phosphatases and protein kinases play a dynamic antagonistic role in protein phosphorylation. Protein phosphatases make up a significant fraction of eukaryotic proteome. In this article, we report the identification and analysis of protein phosphatases in the intracellular parasite Entamoeba histolytica. Based on an in silico analysis, we classified 250 non-redundant protein phosphatases in E. histolytica. The phosphotome of E. histolytica is 3.1% of its proteome and 1.3 times of the human phosphotome. In this extensive study, we identified 42 new putative phosphatases (39 hypothetical proteins and 3 pseudophosphatases). The presence of pseudophosphatases may have an important role in virulence of E. histolytica. A comprehensive phosphotome analysis of E. histolytica shows spectacular low similarity to human phosphatases, making them potent candidates for drug target.
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Tamanna
T
Anwar
Samudrala
S
Gourinath
D004748:Entamoeba histolytica / Q000201:enzymology* / Q000235:genetics; D058977:Molecular Sequence Annotation; D010749:Phosphoprotein Phosphatases / Q000737:chemistry; D010750:Phosphoproteins / Q000737:chemistry*; D010766:Phosphorylation; D010802:Phylogeny; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D020543:Proteome / Q000737:chemistry; D015800:Protozoan Proteins / Q000737:chemistry* / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D037521:Virulence Factors / Q000737:chemistry
D010750:Phosphoproteins; D020543:Proteome; D015800:Protozoan Proteins; D037521:Virulence Factors; D010749:Phosphoprotein Phosphatases