Crambescidin-816 acts as a fungicidal with more potency than crambescidin-800 and -830, inducing cell cycle arrest, increased cell size and apoptosis in Saccharomyces cerevisiae.
In this paper, we show the effect of crambescidin-816, -800, and -830 on Saccharomyces cerevisiae viability. We determined that, of the three molecules tested, crambescidin-816 was the most potent. Based on this result, we continued by determining the effect of crambescidin-816 on the cell cycle of this yeast. The compound induced cell cycle arrest in G2/M followed by an increase in cell DNA content and size. When the type of cell death was analyzed, we observed that crambescidin-816 induced apoptosis. The antifungal effect indicates that crambescidins, and mostly crambescidin-816, could serve as a lead compound to fight fungal infections.
Department of Pharmacology, Faculty of Veterinary, University of Santiago de Compostela (USC), Campus Lugo, Lugo 27002, Spain. luis.botana@usc.es.
Juan A
JA
Rubiolo
Eva
E
Ternon
Henar
H
López-Alonso
Olivier P
OP
Thomas
Félix V
FV
Vega
Mercedes R
MR
Vieytes
Luis M
LM
Botana
D000470:Alkaloids / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D016923:Cell Death / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D005659:Fungicides, Industrial / Q000494:pharmacology*; D016195:G2 Phase / Q000187:drug effects; D019791:Guanidine / Q000031:analogs & derivatives / Q000494:pharmacology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects*; D013141:Spiro Compounds / Q000494:pharmacology*
D000470:Alkaloids; D005659:Fungicides, Industrial; D013141:Spiro Compounds; C490355:crambescidin 800; C083155:crambescidin 816; D019791:Guanidine