Development and validation of a quick easily used biochemical assay for evaluating the viability of small immobile arthropods.
Quickly, accurately, and easily assessing the efficacy of treatments to control sessile arthropods (e.g., scale insects) and stationary immature life stages (e.g., eggs and pupae) is problematic because it is difficult to tell whether treated organisms are alive or dead. Current approaches usually involve either maintaining organisms in the laboratory to observe them for development, gauging their response to physical stimulation, or assessing morphological characters such as turgidity and color. These can be slow, technically difficult, or subjective, and the validity of methods other than laboratory rearing has seldom been tested. Here, we describe development and validation of a quick easily used biochemical colorimetric assay for measuring the viability of arthropods that is sufficiently sensitive to test even very small organisms such as white fly eggs. The assay was adapted from a technique for staining the enzyme hexokinase to signal the presence of adenosine triphosphate in viable specimens by reducing a tetrazolium salt to formazan. Basic laboratory facilities and skills are required for production of the stain, but no specialist equipment, expertise, or facilities are needed for its use.
AgResearch, Lincoln, Private Bag 4749, Canterbury 8140, New Zealand. craig.phillips@agresearch.co.nz
Craig B
CB
Phillips
Ilia I
II
Iline
Nicola K
NK
Richards
Max
M
Novoselov
Mark R
MR
McNeill
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001181:Arthropods / Q000201:enzymology / Q000469:parasitology* / Q000502:physiology*; D003124:Colorimetry / Q000379:methods*; D004396:Coloring Agents / Q000378:metabolism; D005562:Formazans / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D010572:Pest Control, Biological / Q000379:methods*; D013194:Staining and Labeling; D013778:Tetrazolium Salts / Q000378:metabolism
D004396:Coloring Agents; D005562:Formazans; D013778:Tetrazolium Salts; D000255:Adenosine Triphosphate; D006593:Hexokinase