[Development of full-quantified HPLC fingerprint for quality evaluation of ophiopogonis radix of sichuan].
OBJECTIVE
To establish HPLC fingerprint of Ophiopogonis Radix of Sichuan and simultaneously determine two homoisoflavonoids (methylophiopogonanones A and B).


METHODS
Full-quantified HPLC fingerprint was used to establish the HPLC fingerprint and determine the active ingredients of the daodi medicinal material Ophiopogonis Radix of Sichuan in Shengmai injection. Chromatographic condition was as follows: The analytical column was Waters symmetry shield RP 18 (4.6 mm x 250 mm, 5 microm) with a pre-column of symmetry shield RP 18. The mobile phase was acetonitrile-water (containing 0.1% phosphoric acid) with gradient elution. The flow rate was 1.0 mL/min, the detection wavelength was set at 280 nm and the column temperature was maintained at 30 degrees C.


RESULTS
The HPLC fingerprint of Ophiopogonis Radix of Sichuan was established with good separation and repeatability. 24 common peaks were defined in the HPLC fingerprint. The similarity among batches was more than 0.98. Compared with standard reference substances, No. 14 peak was methylophiopogonanone A and No. 15 peak was methylophiopogonanone B. Similarity determine system was applied to evaluate them.


CONCLUSIONS
This analytical method is highly sensitive with strong specificity, which can be used efficiently in the quality control of Ophiopogonis Radix of Sichuan in Shengmai injection.
Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China. wang6593536@163.com
Jin
J
Wang
Han-Qing
HQ
Liu
Lin
L
Liu
Hai-Tao
HT
Tang
Ping
P
Zhang
Yun
Y
Tang
D052117:Benzodioxoles / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000592:standards; D007529:Isoflavones / Q000032:analysis*; D031441:Ophiopogon / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results
D052117:Benzodioxoles; D004365:Drugs, Chinese Herbal; D007529:Isoflavones; C543188:methylophiopogonanone A; C520229:methylophiopogonanone B