Neuroprotective benzyl benzoate glycosides from Disporum viridescens roots in HT22 hippocampal neuronal cells.
Bioassay-guided fractionation of the EtOAc extract from Disporum viridescens roots led to the isolation of five new benzyl benzoate glycosides, BBGs (1-5). The neuroprotective activities of the BBGs were screened using neuronal HT22 hippocampal cells. BBG-D (4) significantly protected murine hippocampal HT22 cells against glutamate-induced neurotoxicity by maintaining the antioxidative defense systems such as superoxide dismutase, glutathione reductase, glutathione peroxidase, and the glutathione content. BBG-D, in a dose-and time-dependent manner, increased HO-1 expression through the selective activation of pERK signaling among the MAPK pathways. These results suggest that BBG-D could be a promising candidate for the treatment of neurodegenerative diseases related to glutamate-induced oxidative neuronal cytotoxicity.
College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University , Seoul 151-742, Republic of Korea.
Namki
N
Cho
Heejung
H
Yang
Mina
M
Lee
Jungmoo
J
Huh
Hyeon-Woo
HW
Kim
Hong-Pyo
HP
Kim
Sang-Hyun
SH
Sung
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D001565:Benzoates / Q000737:chemistry / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D018698:Glutamic Acid / Q000378:metabolism / Q000494:pharmacology*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D051547:Heme Oxygenase-1 / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D019668:Liliaceae / Q000737:chemistry*; D051379:Mice; D015394:Molecular Structure; D009474:Neurons / Q000378:metabolism; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D018517:Plant Roots / Q000737:chemistry; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism
D000975:Antioxidants; D001565:Benzoates; D006027:Glycosides; D018696:Neuroprotective Agents; D018698:Glutamic Acid; D051547:Heme Oxygenase-1; D013482:Superoxide Dismutase; C006723:benzyl benzoate