Intact cell/intact spore mass spectrometry (IC/ISMS) on polymer-based, nano-coated disposable targets.
Identification and differentiation of microorganisms has and still is a long arduous task, involving culturing of the organism in question on different growth media. This procedure, which is still commonly applied, is an established method, but takes a lot of time, up to several days or even longer. It has thus been a great achievement when other analytical tools like matrix-assisted laser desorption/ionization (MALDI) mass spectrometry were introduced for faster analysis based on the surface protein pattern. Differentiation and identification of human pathogens as well as plant/animal pathogens is of increasing importance in medical care (e.g. infection, sepsis, and antibiotics resistance), biotechnology, food sciences and detection of biological warfare agents. A distinction between microorganisms on the species and strain level was made by comparing peptide/protein profiles to patterns already stored in databases. These profiles and patterns were obtained from the surface of vegetative forms of microorganisms or even their spores by MALDI MS. Thus, an unknown sample can be compared against a database of known pathogens or microorganisms of interest. To benefit from newly available, metal-based disposable microscope-slide format MALDI targets that promise a clean and even surface at a fraction of the cost from full metal targets or MTP (microtiter plate) format targets, IC/ISMS analysis was performed on these and the data evaluated. Various types of bacteria as well as fungal spores were identified unambiguously on this disposable new type of metal nano-coated targets. The method even allowed differentiation between strains of the same species. The results were compared with those gained from using full metal standard targets and found to be equal or even better in several aspects, making the use of disposable MALDI targets a viable option for use in IC/ISMS, especially e.g. for large sample throughput and highly pathogenic species.
Institute of Chemical Technologies and Analytics, Vienna University of Technology, Getreidemarkt 9/164, Vienna A-1060, Austria.
Stefan
S
Bugovsky
Institute of Chemical Technologies and Analytics, Vienna University of Technology, Getreidemarkt 9/164, Vienna A-1060, Austria.
Wolfgang
W
Winkler
Sony DADC, BioSciences Business Unit, Anif, Austria.
Werner
W
Balika
Sony DADC, BioSciences Business Unit, Anif, Austria.
Manfred
M
Koranda
Institute of Chemical Technologies and Analytics, Vienna University of Technology, Getreidemarkt 9/164, Vienna A-1060, Austria. Electronic address: guenter.allmaier@tuwien.ac.at.
Günter
G
Allmaier
D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D005658:Fungi / Q000145:classification / Q000302:isolation & purification*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000191:economics; D011108:Polymers; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods*; D013170:Spores / Q000145:classification / Q000302:isolation & purification
D011108:Polymers
Bacteria; Biotyping; Disposable polymer-based target; Fungi; Intact cell mass spectrometry; MALDI mass spectrometry