Genomic changes in rectal adenocarcinoma associated with liver metastasis.
BACKGROUND
At present no objective parameters to identify the risk of liver metastasis after surgery have been established in rectal cancer.


OBJECTIVE
To identify the chromosomal aberrations that are correlated with liver metastasis of rectal cancer.


METHODS
Primary tumor tissues of rectal carcinoma were analyzed by array-based comparative genomic hybridization (array-CGH). Genomic aberrations were identified by Genomic Workbench and MD-SeeGH.


RESULTS
The most frequent gains in rectal cancer were at 20q11.21-q13.33, 8q11.21-q24.3, 13q12.11-q14.2 and losses in 5q13.2, 8p23.3-p22, 17p13.3-p13.2 and 18q11.2-q23. Seven amplifications at 6p21.1, 8q24.21, 8q24.3, 13q13.2 and 20q13.2-q13.32 and nine homozygous deletions at 1q31.3, 4q12-q13.1, 4q32.3-q33, 5q13.2, 8p23.2, 8q11.23, 16p13.2, 19p13.11 and 19q13.41 were identified. Both frequency plot comparison and SAM (Significance analysis of microarray) methods indicated that losses at 1p35.3, 4p14, 14q23.1-q32.11 and 18p11.32-p11.21 were more frequent in patients without liver metastasis.


CONCLUSIONS
These liver metastasis associated genomic changes may be useful to reveal the mechanism of metastasis and identify candidate biomarkers.
Department of Abdominal Surgery, Cancer Hospital/Institute, Chinese Academy of Medical Science, Peking Union Medical College, Beijing, China.
Hai-Tao
HT
Zhou
Zhi-Zhou
ZZ
Shi
Zhi-Xiang
ZX
Zhou
Yan-Yi
YY
Jiang
Jia-Jie
JJ
Hao
Tong-Tong
TT
Zhang
Feng
F
Shi
Xin
X
Xu
Ming-Rong
MR
Wang
Yu
Y
Zhang
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology*; D000368:Aged; D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D055028:Comparative Genomic Hybridization; D056915:DNA Copy Number Variations; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000556:secondary*; D008297:Male; D008875:Middle Aged; D012004:Rectal Neoplasms / Q000235:genetics* / Q000473:pathology*
Array CGH; biomarker; liver metastasis; rectal cancer