[Optimization of extraction process of prescription of compound Nanxing pain-relieving cataplasm by combining orthogonal design and pharmacodynamic experiment].
OBJECTIVE
To discuss the optimum extraction process of compound Nanxing pain-relieving cataplasm through orthogonal design and pharmacodynamic experiment


METHODS
The orthogonal experiment method was adopted to optimize the ethanol extraction process with Angelica dahurica, Ligusticum chuanxiong and other herbs. The anti-inflammatory and analgesic effects of extracts from volatile oil in such herbs as syzygium aromaticum with different extraction processes were compared by tail pain tenderness test and food-pad swelling in mice, in order to optimize the extraction process of extracts from volatile oil in such herbs as syzygium aromaticum.


RESULTS
The optimum extraction process of A. dahurica, L. chuanxiong and other herbs for compound Nanxing pain-relieving cataplasm were as follows: adding 8-fold amount of 70% alcohol, extracting for 2 times with 1.5 h each time. The 95% ethnol extracts of syzygium aromaticum and other herbs had more effect in the increasing the threshold of pain and the inhibition of toe swelling of mice than volatile oil obtained from steam distillation as well as volatile oil and water decoction obtained from steam distillation.


CONCLUSIONS
The method is simple and reliable that it can provide technical reference for the development of modern preparations of compound Nanxing pain-relieving cataplasm.
Beijing University of Chinese Medicine, Beijing 100102, China. lysole@126.com
Ying
Y
Li
Shou-Ying
SY
Du
Yang
Y
Lu
Jie
J
Bai
Pan
P
Xu
Ying-Zi
YZ
Wang
Yue
Y
Wang
D000700:Analgesics / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D000431:Ethanol / Q000737:chemistry; D051379:Mice; D009822:Oils, Volatile / Q000737:chemistry; D010146:Pain / Q000188:drug therapy*
D000700:Analgesics; D004365:Drugs, Chinese Herbal; D009822:Oils, Volatile; C585598:nanxing; D000431:Ethanol