High-resolution microscopy for biological specimens via cathodoluminescence of Eu- and Zn-doped Y2O3 nanophosphors.
High-resolution microscopy for biological specimens was performed using cathodoluminescence (CL) of Y(2)O(3):Eu, Zn nanophosphors, which have high CL intensity due to the incorporation of Zn. The intensity of Y(2)O(3):Eu nanophosphors at low acceleration voltage (3 kV) was increased by adding Zn. The CL intensity was high enough for imaging even with a phosphor size as small as about 30 nm. The results show the possibility of using CL microscopy for biological specimens at single-protein-scale resolution. CL imaging of HeLa cells containing laser-ablated Y(2)O(3):Eu, Zn nanophosphors achieved a spatial resolution of a few tens of nanometers. Y(2)O(3):Eu, Zn nanophosphors in HeLa cells were also imaged with 254 nm ultraviolet light excitation. The results suggest that correlative microscopy using CL, secondary electrons and fluorescence imaging could enable multi-scale investigation of molecular localization from the nanoscale to the microscale.
Taichi
T
Furukawa
Hirohiko
H
Niioka
Masayoshi
M
Ichimiya
Tomohiro
T
Nagata
Masaaki
M
Ashida
Tsutomu
T
Araki
Mamoru
M
Hashimoto
D003287:Contrast Media; D006367:HeLa Cells; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D008029:Lighting / Q000379:methods; D008163:Luminescent Measurements / Q000379:methods*; D053768:Metal Nanoparticles; D008856:Microscopy, Fluorescence / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D009363:Neoplasm Proteins / Q000378:metabolism* / Q000648:ultrastructure*
D003287:Contrast Media; D009363:Neoplasm Proteins