Effects of the enamel matrix derivative on the proliferation and odontogenic differentiation of human dental pulp cells.
OBJECTIVE
The enamel matrix derivative (EMD) has a positive effect on the proliferation of human periodontal ligament cells and the healing of periodontal tissues. The aim of this study was to evaluate the effects of EMD on the proliferation and differentiation of human dental pulp cells (hDPCs) in vitro.


METHODS
hDPCs were isolated from human impacted third molars and cultured in vitro. After treatment with100μg/mL EMD, the proliferation of hDPCs was determined by a cell counting kit 8 (CCK8) assay. After incubation in EMD osteogenic induction medium for 14 days, the osteogenic differentiation of hDPCs was evaluated by alkaline phosphatase (ALP) activity, alizarin staining and the expression of osteogenesis-related genes.


RESULTS
The EMD osteogenic induction medium enhanced the proliferation of hDPCs. After osteogenic induction, EMD increased the osteogenic potential of hDPCs, as measured by alkaline phosphatase activity and calcium accumulation; the expression levels of osteogenesis-related genes, such as ALP, DSPP, BMP, and OPN were also upregulated. In addition, the expression levels of odontogenesis-related transcription factors Osterix and Runx2 were upregulated.


CONCLUSIONS
EMD could enhance the mineralization of hDPSCs upregulated the expression of markers for odontoblast/osteoblast-like cells. Further studies are required to determine if EMD can improve pulp tissue repair and regeneration.
School and Hospital of Stomatology, Peking University, Beijing, China.
Yuanyuan
Y
Wang
School and Hospital of Stomatology, Peking University, Beijing, China. Electronic address: yumingzhao70@gmail.com.
Yuming
Y
Zhao
School and Hospital of Stomatology, Peking University, Beijing, China.
Lihong
L
Ge
D000469:Alkaline Phosphatase / Q000032:analysis; D000880:Anthraquinones; D019485:Bone Morphogenetic Proteins / Q000032:analysis; D002113:Calcification, Physiologic / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002452:Cell Count; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis; D003470:Culture Media; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D016326:Extracellular Matrix Proteins / Q000032:analysis; D006801:Humans; D008297:Male; D009805:Odontogenesis / Q000187:drug effects*; D053495:Osteopontin / Q000032:analysis; D010750:Phosphoproteins / Q000032:analysis; D012795:Sialoglycoproteins / Q000032:analysis; D000076182:Sp7 Transcription Factor; D014157:Transcription Factors / Q000032:analysis; D055815:Young Adult; D000099311:Dentin Sialophosphoprotein
D000469:Alkaline Phosphatase; D000880:Anthraquinones; D019485:Bone Morphogenetic Proteins; D002118:Calcium; D004396:Coloring Agents; D050659:Core Binding Factor Alpha 1 Subunit; D003470:Culture Media; D003746:Dental Enamel Proteins; D016326:Extracellular Matrix Proteins; D053495:Osteopontin; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000076182:Sp7 Transcription Factor; D014157:Transcription Factors; D000099311:Dentin Sialophosphoprotein; C493709:RUNX2 protein, human; C478163:SP7 protein, human; C109069:enamel matrix proteins; C010078:alizarin
Dental pulp cells; Differentiation; Enamel matrix derivative; Odontogenesis