An optimization procedure for the isocratic high-performance liquid chromatographic separations of environmental pollutants.
This paper describes the use of the overlapping resolution mapping procedure to optimize the separation of priority pollutants by isocratic, reversed-phase high-performance liquid chromatography. Three classes of pollutants are considered: polycyclic aromatic hydrocarbons, substituted phenols and phthalate esters. The procedure requires only seven chromatographic runs for each set of pollutants before an optimized mobile phase composition is derived which can satisfactorily resolve all the components in the respective mixtures of pollutants. The main advantage of such a systematic scheme to optimize mobile phase compositions is an improvement in method development times compared to conventional techniques. Another benefit is that expenditures on solvents are reduced.
Department of Chemistry, National University of Singapore, Kent Ridge, 0511, Republic of Singapore.
C P
CP
Ong
M R
MR
Khan
S F
SF
Li
H K
HK
Lee