Characterization of the noncoding regions of the 1918 influenza A H1N1 virus.
The terminal noncoding region (NCR) sequences of the eight gene segments of the influenza A/Brevig Mission/1/1918 (H1N1) virus were determined by rapid amplification of cDNA ends (RACE). Chimeric viruses encoding the open reading frames of the 1918 virus but flanked by either the wild-type 1918 NCR sequences or the NCR sequences of two other H1N1 virus strains, A/WSN/1933 and A/New York/312/2001, were produced. No growth differences between the NCR variant 1918 influenza viruses were noted.
Viral Pathogenesis and Evolution Section, Laboratory of Infectious Diseases, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
Ruixue
R
Wang
Jeffery K
JK
Taubenberger
D001483:Base Sequence; D002460:Cell Line; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D007251:Influenza, Human / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020506:Untranslated Regions; D014779:Virus Replication
D012367:RNA, Viral; D020506:Untranslated Regions