Neutralizing monoclonal antibodies to the E2 protein of chikungunya virus protects against disease in a mouse model.
Chikungunya virus (CHIKV) recently caused the largest epidemic ever recorded for this virus involving an estimated 1.4-6.5million cases, with imported cased reported in over 40 countries. The number of monoclonal antibodies specific for this re-emerging alphavirus is currently limited. Herein we describe the generation and characterisation of five monoclonal antibodies specific for the E2 glycoprotein of CHIKV. The antibodies detected a range of CHIKV isolates in several assays including ELISA, Western blot, immunofluorescence assay (IFA) and immunohistochemistry (IHC) without evidence of cross-reactivity with other alphaviruses. Four antibodies also neutralised CHIKV in vitro, two of which provided complete protection against arthritis in a CHIKV mouse model when administered prior to infection. Given the current shortage of widely available reagents for CHIKV, these specific antibodies will be useful not only in research, but may also provide the basis for new diagnostics and treatments.
Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Queensland 4072, Australia.
Lucas Y H
LY
Goh
Jody
J
Hobson-Peters
Natalie A
NA
Prow
Joy
J
Gardner
Helle
H
Bielefeldt-Ohmann
Alyssa T
AT
Pyke
Andreas
A
Suhrbier
Roy A
RA
Hall
D018354:Alphavirus Infections / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D057134:Antibodies, Neutralizing / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D001169:Arthritis, Experimental / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D019556:COS Cells; D002646:Chikungunya virus / Q000187:drug effects / Q000276:immunology; D002522:Chlorocebus aethiops; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D014759:Viral Envelope Proteins
Chikungunya virus; Diagnostic; Disease protection; ELISA; Immunohistochemistry; Monoclonal antibodies