Allospecific CD8 T suppressor cells induced by multiple MLC stimulation or priming in the presence of ILT3.Fc have similar gene expression profiles.
Alloantigen specific CD8 T suppressor cells can be generated in vitro either by multiple stimulations of CD3 T cells with allogeneic APC or by single stimulation in primary MLC containing recombinant ILT3.Fc protein. The aim of the present study was to determine whether multiple MLC stimulation induced in CD8(+) CD28(-) T suppressor cells molecular changes that are similar to those observed in CD8 T suppressor cells from primary MLC containing ILT3.Fc protein. Our study demonstrates that the characteristic signatures of CD8 T suppressor cells, generated by either of these methods are the same consisting of up-regulation of the BCL6 transcriptional repressor and down-regulation of inflammatory microRNAs, miR-21, miR-30b, miR-146a, and miR-155 expression. In conclusion microRNAs which are increased under inflammatory conditions in activated CD4 and CD8 T cells with helper or cytotoxic function show low levels of expression in CD8 T cells which have acquired antigen-specific suppressor activity.
Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, United States; Department of Cardiology, The First People's Hospital of Jiujiang, Jiujiang Affiliated Hospital, Nanchang University, Jiujiang, Jiangxi 332000, China.
Ling
L
Chen
Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, United States.
Zheng
Z
Xu
Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, United States.
Chris
C
Chang
Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, United States.
Sophey
S
Ho
Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, United States.
Zhuoru
Z
Liu
Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, United States.
George
G
Vlad
Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, United States.
Raffaello
R
Cortesini
Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, United States.
Raphael A
RA
Clynes
Department of Cardiology, The First People's Hospital of Jiujiang, Jiujiang Affiliated Hospital, Nanchang University, Jiujiang, Jiangxi 332000, China.
Yun
Y
Luo
Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, United States. Electronic address: ns20@columbia.edu.
Nicole
N
Suciu-Foca
D017252:CD3 Complex / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006801:Humans; D007108:Immune Tolerance; D007141:Immunoglobulin Fc Fragments / Q000235:genetics / Q000378:metabolism*; D018836:Inflammation Mediators / Q000378:metabolism; D007519:Isoantigens / Q000276:immunology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008562:Membrane Glycoproteins; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011971:Receptors, Immunologic; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D059467:Transcriptome
C411984:BCOR protein, human; D017252:CD3 Complex; D016827:CD8 Antigens; D007141:Immunoglobulin Fc Fragments; D018836:Inflammation Mediators; D007519:Isoantigens; C107907:LILRB4 protein, human; D008562:Membrane Glycoproteins; D035683:MicroRNAs; D011518:Proto-Oncogene Proteins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins
B cell CLL/lymphoma 6; BCL6; T regulatory cells; T suppressor cells; Treg; Ts; miR; microRNA