Flux variability analysis: application to developing oilseed rape embryos using toolboxes for constraint-based modeling.
Flux variability analysis enables comprehensive exploration of alternate optimal routes in a metabolic network. This method is especially useful with models such as bna572 for the developing oilseed rape embryo which is highly compartmentalized. Here, we describe a protocol for carrying out flux variability analysis on reactions and network projections of bna572 using well-established software (CellNetAnalyzer and COBRA) for constraint-based analysis of stoichiometric network reconstructions.
Biosciences Department, Brookhaven National Laboratory, Upton, NY, USA.
Jordan O
JO
Hay
Jörg
J
Schwender
D029688:Brassica napus / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D003198:Computer Simulation; D064688:Metabolic Flux Analysis; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D012639:Seeds / Q000378:metabolism*; D012984:Software