Sex-specific expression and localization of aromatase and its regulators during embryonic and larval development of Atlantic salmon.
The products of dax1, foxl2a and mis have each been shown to have proliferative and/or differentiative activities during mammalian organogenesis. These factors also play a role in regulating the biosynthesis of estrogen, particularly by modulating the activity of aromatase cyp19a. We demonstrate the transcription and translation of these genes during salmon embryogenesis. We were able to track sex-specific differences in these processes through accurate determination of the sex of each embryo and larva examined from genotyped microsatellites. We detected sex- and stage-specific immunolabeling of the embryonic gut, kidney, gonads, neural cord and skeletal muscle by DAX-1, FOXL2A and MIS. These results indicate the potential of these factors to mediate proliferation and/or differentiation programs during development of these tissues. As well, immunolabeling of skeletal muscle by CYP19B1 throughout the study reveals probable neurogenic activity associated with peripheral radial glial cells and the growing embryonic musculature.
Department of Biology, Centre for Biomedical Research, University of Victoria, Victoria, British Columbia V8W 3N5, Canada; Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada. Electronic address: krvs@uvic.ca.
Kristian R
KR
von Schalburg
Department of Biology, Electron Microscopy Laboratory, University of Victoria, Victoria, British Columbia V8W 3N5, Canada.
Brent E
BE
Gowen
Department of Biology, Centre for Biomedical Research, University of Victoria, Victoria, British Columbia V8W 3N5, Canada.
Amber M
AM
Messmer
Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada.
William S
WS
Davidson
Department of Biology, Centre for Biomedical Research, University of Victoria, Victoria, British Columbia V8W 3N5, Canada.
Ben F
BF
Koop
D000818:Animals; D001141:Aromatase / Q000235:genetics* / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005838:Genotype; D007814:Larva / Q000201:enzymology / Q000254:growth & development; D008297:Male; D008648:Mesoderm / Q000196:embryology / Q000201:enzymology / Q000254:growth & development; D009024:Morphogenesis; D018482:Muscle, Skeletal / Q000196:embryology / Q000201:enzymology; D020317:Salmo salar / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D012737:Sex Factors
D001141:Aromatase
Gonad; Gut; Kidney; Neural cord; Skeletal muscle