Expression and localization of osteopontin, homing cell adhesion molecule/CD44, and integrin αvβ3 in pleomorphic adenoma, polymorphous low-grade adenocarcinoma, and adenoid cystic carcinoma.
OBJECTIVE
Osteopontin (OPN) plays a role in tumor progression. This study aimed to determine the expression of OPN, CD44, and integrin αvβ3 in pleomorphic adenoma (PA), polymorphous low-grade adenocarcinoma (PLGA), and adenoid cystic carcinoma (ACC).


METHODS
Immunohistochemistry was used to semiquantify the level of expression of OPN and its receptors in normal salivary glands (NSG; n = 20), PA (n = 20), PLGA (n = 16), and ACC (n = 22).


RESULTS
OPN expression was increased in PLGA and intermediate-/high-grade ACC compared with PA and NSG (median scores, 6, 5, 4, and 4, respectively). CD44 expression was reduced in PA, PLGA, and ACC. OPN expression levels were moderately correlated with CD44 in PLGA. Integrin αvβ3 was not expressed in PA and ACC and was seen in only 1 case of PLGA.


CONCLUSIONS
OPN is expressed in salivary gland tumors but does not correlate well with CD44 and αvβ3.
Oral and Maxillofacial Surgery Resident, Division of Oral Surgery, Schulich School of Medicine and Dentistry, Western University, London, Ontario, Canada.
T C
TC
Fok
H
H
Lapointe
A B
AB
Tuck
A F
AF
Chambers
L
L
Jackson-Boeters
T D
TD
Daley
M R
MR
Darling
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D008949:Adenoma, Pleomorphic / Q000378:metabolism* / Q000473:pathology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D005260:Female; D006258:Head and Neck Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D039302:Integrin alphaVbeta3 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D053495:Osteopontin / Q000378:metabolism*; D012469:Salivary Glands / Q000378:metabolism
C497483:CD44 protein, human; D015815:Cell Adhesion Molecules; D018960:Hyaluronan Receptors; D039302:Integrin alphaVbeta3; D053495:Osteopontin