Amplified plasmonic detection of DNA hybridization using doxorubicin-capped gold particles.
We show in this article that doxorubicin-modified gold nanoparticles (Au NP-DOX) can be used for the post-amplification of the wavelength shift of localized surface plasmon resonance (LSPR) signals after DNA hybridization events. We take advantage of the intercalation properties of DOX with guanine-rich oligonucleotides and the plasmon coupling between surface-linked gold nanostructures and Au NP-DOX in solution to detect in a sensitive manner DNA hybridisation events. Post-treatment of double-stranded DNA with Au NP-DOX resulted in a detection limit of ≈600 pM, several times lower than that without post-incubation (LOD ≈ 40 nM).
Laboratoire de Réactivité de Surfaces, UMR CNRS 7197, Université Pierre & Marie Curie - Paris VI, Site d'Ivry - Le Raphaël, 94200 Ivry-sur-Seine, France. jolanda.spadavecchia@upmc.fr sabine.szunerits@iri.univ-lille1.fr.
Jolanda
J
Spadavecchia
Ramesh
R
Perumal
Alexandre
A
Barras
Joel
J
Lyskawa
Patrice
P
Woisel
William
W
Laure
Claire-Marie
CM
Pradier
Rabah
R
Boukherroub
Sabine
S
Szunerits
D015342:DNA Probes / Q000737:chemistry*; D004317:Doxorubicin / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*
D015342:DNA Probes; D006046:Gold; D004317:Doxorubicin