Protein bodies in ray cells of Populus x canadensis Moench 'robusta'.
Light- and electron-microscopical investigations revealed distinct intravacuolar protein aggregates of 0.3-0.8 μm in diameter in ray cells of poplar during the dormant season. In semi-thin sections, these bodies showed positive protein staining and enzymatic digestibility with pepsin, indicating their proteinaceous nature. Morphometric measurements showed such protein bodies in 7-13% of the area of the ray-cell lumen. This amount corresponded with the protein content of the wood determined biochemically, e.g. 2.0-5.0 μg·mg(-1) dry weight. Polyacrylamide gel electrophoresis of the total protein fraction extracted from wood showed prominent polypeptide species with an apparent molecular weight of 30-32 kilodaltons. The results indicate considerable protein storage in ray cells, especially in the form of protein-storage vacuoles.
Botanisches Institut der Universität Kiel, Olshausenstrasse 40, D-2300, Kiel, Federal Republic of Germany.
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Sauter
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van Cleve
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Apel