Anaerostipes rhamnosivorans sp. nov., a human intestinal, butyrate-forming bacterium.
A novel butyrate-producing bacterium, strain 1y-2(T), was isolated from a stool sample of a 1-year-old, healthy Dutch infant. The isolate was obtained by using lactate and acetate as sources of carbon and energy. The strain was Gram-variable, strictly anaerobic and spore-forming and formed curly rod-shaped cells that fermented glucose into butyrate, lactate, formate and acetate as main products. The DNA G+C content of the strain was 44.5 mol% and its major cellular fatty acids were C12:0, iso-C19:1 I and C16:0. Strain 1y-2(T) was related to Anaerostipes caccae DSM 14662(T) based on 16S rRNA gene sequence analysis, with 3% divergence, but hybridization studies of their genomic DNA revealed only 33% relatedness. Moreover, strain 1y-2(T) showed marked physiological and biochemical differences from known species of the genus Anaerostipes. Based on phylogenetic, chemotypic and phenotypic criteria, we propose that strain 1y-2(T) should be classified in the genus Anaerostipes within a novel species, Anaerostipes rhamnosivorans sp. nov. The type strain is 1y-2(T) ( = DSM 26241(T) = KCTC 15316(T)).
Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
Thi Phuong Nam
TPN
Bui
Department of Basic Veterinary Medicine and Department of Bacteriology and Immunology, University of Helsinki, Helsinki, Finland.|Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
Willem M
WM
de Vos
Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
Caroline M
CM
Plugge
D001482:Base Composition; D002087:Butyrates / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D016937:Gram-Positive Endospore-Forming Rods / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007223:Infant; D007422:Intestines / Q000382:microbiology*; D008969:Molecular Sequence Data; D009426:Netherlands; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D002087:Butyrates; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S