The influence of auxins, light and cell differentiation on solasodine production bySolanum eleagnifolium Cav. calli.
The effect of auxins, light and cellular production ofSolanum eleagnifolium Cav. calli were studied. 2,4-dichlorophenoxyacetic acid (4.5 μM) was the plant growth regulator used for calli initiation and this produced the highest solasodine concentration. The solasodine concentration in darkness was significantly lower than that achieved under a photoperiod of 16 h. Differentiated tissue obtained by adequate hormonal balance (several ratios of 3-indolebutyric acid to 6-benzylaminopurine) produced higher yields of solasodine than non-differentiated tissue. 3-indolebutyric acid (2.5 μM) and 6-benzylaminopurine (8.8 μM) increased the productivity of solasodine by 100%.
Biotecnologia, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, (1113) Junín 956, Buenos Aires, República Argentina.
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HM
Nigra
M A
MA
Alvarez
A M
AM
Giulietti