Transfection of germinating barley seed electrophoretically with exogenous DNA.
A method is described for transfection (genetic transformation) of barley caryopsis electrophoretically with DNA. β-Glucuronidase activity was detected after the electrophoretic transfection with plasmid pBI221 DNA carrying the cauliflower mosaic virus promotor and bacterial β-glucuronidase coding sequence. Electrophoretic transfection is evidently effective with pieces of callus and seeds of many plants.
Department of Genetics and Plant Molecular Biology Laboratory, University of Helsinki, Arkadiankatu 7, SF-00100, Helsinki, Finland.
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Ahokas