Association of plasma viremia with HIV-1 RNA levels in cervicovaginal lavage secretions in HIV-1 seropositive ART naïve women from Pune, India.
BACKGROUND
Coherent drug/microbicide/vaccine development research would benefit through a precise knowledge of HIV dissemination and its persistence in the female genital tract. Understanding relationship between plasma viremia and cervicovaginal HIV shedding may help to unveil mechanisms underlying transmission, compartmentalization and pathogenesis.


OBJECTIVE
To study the association between HIV-1 RNA levels in the plasma and CVL specimens.


METHODS
Whole blood, plasma and CVL specimens were collected from 36 ART naïve HIV-1 seropositive women qualifying the study inclusion criteria. Absolute CD4 counts, plasma and CVL HIV-1 RNA levels were estimated using commercially available kits (BD MultiSET™ Kit, Becton Dickinson, US and Abbott RT, Abbott Molecular, Germany). Correlation between plasma and CVL viral load was estimated by the Spearman's rank correlation coefficient. Additionally, the correlation between CVL viral load and absolute CD4 counts was studied.


RESULTS
HIV-1 viral load in the CVL specimens was successfully quantified using the Abbott RT. Twenty-seven of 36 women (75%) had detectable HIV-1 RNA levels in plasma and CVL specimens. The CVL viral load did not show any correlation with plasma viral load (ρ=0.281, p=0.096) and showed a 'moderate correlation' (ρ=-0.563, p=0.0004) with absolute CD4 counts.


CONCLUSIONS
Albeit, the Abbott RT is designed for estimating plasma HIV-1 RNA levels, the study reports its use for estimating HIV-1 RNA levels in the CVL specimens as well. In accordance with the previous studies, our results suggest that plasma and CVL viral load are not correlated and plasma viremia might not solely predict cervico vaginal HIV shedding.
National AIDS Research Institute, 73, 'G' Block, MIDC, Bhosari, Pune 411026, India.
Priyanka
P
Khopkar
Suvarna
S
Sane
Shubhangi
S
Bichare
Amita
A
Verma
Smita
S
Kulkarni
D000293:Adolescent; D000328:Adult; D018791:CD4 Lymphocyte Count; D002584:Cervix Uteri / Q000378:metabolism / Q000821:virology*; D005260:Female; D006679:HIV Seropositivity / Q000097:blood* / Q000453:epidemiology / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007194:India / Q000453:epidemiology; D008875:Middle Aged; D012367:RNA, Viral / Q000097:blood / Q000378:metabolism*; D018709:Statistics, Nonparametric; D014621:Vagina / Q000821:virology*; D019562:Viral Load; D014766:Viremia / Q000097:blood*; D055815:Young Adult
D012367:RNA, Viral
ART; Abbott HIV-1 RealTime™ assay; Abbott RT; CVL; Cervicovaginal lavage; Genital shedding; HIV-1; India; US; United States; Viral load; antiretroviral therapy; cervicovaginal lavage