[Physicochemical properties and skin penetration in vitro of total alkaloids of Sophora flavescens nanoemulsion].
The research aimed at investigating the physicochemical properties, stability and skin penetration in vitro of total alkaloids of Sophora flavescens nanoemulsion. Prepare total alkaloids of S. flavescens nanoemulsion and detect the determination of matrine and oxymatrine in the nanoemulsion using HPLC method. Transmission electron microscopy and laser particle size analyzer were utilized to detect the shape and size of the nanoemulsion respectively. And also the stability of nanoemulsion was studied under the conditions of low temperature (4 degrees C), normal temperature (25 degrees C) and high temperature (60 degrees C). Franz diffusion cell was used to research the transdermal absorption of nanoemulsion in vitro. The results found that the nanoemulsion we prepared presented appearance of rounded, uniform; its average diameter was (15.55 +/- 2.24) nm, and particle size distribution value was 0. 161; the appearance, diameter and percentage determination of total alkaloids of S. flavescens had no variations after 15 d under 4, 25, 60 degrees C respectively. The steady-state permeation rate was 4.564 1 microg x cm(-2) x h(-1), 24 h cumulative amount of penetration was 110.7 microg x cm(-2), which was 1.86 fold of 24 h cumulative amount of aqueous solution (59.41 microg x cm(-2)). All the results demonstrated total alkaloids of S. flavescens nanoemulsion had good permeability, and could provide a new preparation for its clinical application.
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. fengailing6167@sina.com
Ai-Ling
AL
Feng
Ying-Zi
YZ
Wang
Sheng-Hai
SH
Zhang
Xiu-Yu
XY
Sun
Fei-Peng
FP
Duan
Cai-Xia
CX
Li
D000470:Alkaloids / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D055598:Chemical Phenomena; D004337:Drug Carriers / Q000737:chemistry*; D004655:Emulsions; D008297:Male; D049329:Nanostructures / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012869:Skin Absorption; D029909:Sophora / Q000737:chemistry*
D000470:Alkaloids; D004337:Drug Carriers; D004655:Emulsions