Purification and functional characterization of factor I.
Factor I (FI) is a soluble, 88 kDa glycoprotein present in plasma at a concentration of approximately 35 mg/L. FI inhibits all complement pathways as it degrades activated C4b and C3b when these are bound to a cofactor such as C4b-binding protein or factor H. Here, we describe a method for purification of FI from human plasma, which is based on affinity chromatography followed by anion exchange chromatography. We also describe a functional assay, in which activity of FI can be assessed.
Department of Laboratory Medicine, Section of Medical Protein Chemistry, The Wallenberg Laboratory, Lund University, Skåne University Hospital, Malmö, Sweden.
Sara C
SC
Nilsson
Anna M
AM
Blom
D015153:Blotting, Western; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005340:Fibrinogen / Q000302:isolation & purification* / Q000378:metabolism*; D006801:Humans
D005340:Fibrinogen