Functional analysis of CD59 using complement-dependent cytotoxicity assay.
CD59 overexpression has been shown to confer the resistance of tumors to complement lysis. Complement lysis is one of the two major killing mechanisms of therapeutic anticancer antibodies. This chapter provides a method that allows studying the extent of complement protection of tumors by CD59.
Division of Transplantation Immunology and Mucosal Biology, MRC Centre for Transplantation, King's College London, Guy's Hospital, London, UK.
Martin
M
Kolev
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D018957:CD59 Antigens / Q000276:immunology* / Q000378:metabolism*; D002460:Cell Line; D003165:Complement System Proteins / Q000276:immunology* / Q000378:metabolism*; D003602:Cytotoxicity, Immunologic; D006801:Humans; D051379:Mice; D051381:Rats
D000911:Antibodies, Monoclonal; D018957:CD59 Antigens; D003165:Complement System Proteins