An in vitro and in vivo comparison of the effect of Stevia rebaudiana extracts on different caries-related variables: a randomized controlled trial pilot study.
The effect of Stevia extracts on in vitro Streptococcus mutans biofilm formation and in vivo plaque pH was evaluated in this paper. Three 10% solutions containing stevioside, rebaudioside A or sucrose were prepared. MTT assay was used to evaluate microbiological counts in vitro. Twenty volunteers rinsed for 1 min with each solutions, and plaque pH was measured at 7 time points after each rinse. Higher in vitro S. mutans biofilm formation was observed in sucrose solution (p < 0.01). After 5, 10, 15 and 30 min, the sucrose in vivo rinse produced a statistically significantly lower pH value compared to the Stevia extracts (F = 99.45, p < 0.01).Stevia extracts can be considered nonacidogenic.
Department of Health Sciences, University of Milan, Milan, Italy.
E
E
Brambilla
M G
MG
Cagetti
A
A
Ionescu
G
G
Campus
P
P
Lingström
D000328:Adult; D058491:Bacterial Load / Q000187:drug effects; D001431:Bacteriological Techniques; D018441:Biofilms / Q000187:drug effects*; D004396:Coloring Agents; D003731:Dental Caries / Q000382:microbiology*; D003773:Dental Plaque / Q000503:physiopathology*; D045786:Diterpenes, Kaurane / Q000494:pharmacology; D005260:Female; D005500:Follow-Up Studies; D005960:Glucosides / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D009067:Mouthwashes / Q000494:pharmacology; D010865:Pilot Projects; D010936:Plant Extracts / Q000494:pharmacology*; D013053:Spectrophotometry / Q000379:methods; D036625:Stevia; D013295:Streptococcus mutans / Q000187:drug effects* / Q000254:growth & development; D013395:Sucrose / Q000494:pharmacology; D013549:Sweetening Agents / Q000494:pharmacology; D013778:Tetrazolium Salts; D013844:Thiazoles; D055815:Young Adult
D004396:Coloring Agents; D045786:Diterpenes, Kaurane; D005960:Glucosides; D009067:Mouthwashes; D010936:Plant Extracts; D013549:Sweetening Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; C012043:stevioside; D013395:Sucrose; C025474:rebaudioside A; C022616:thiazolyl blue