Permeabilization of cultivated plant cells by electroporation for release of intracellularly stored secondary products.
Plant cell suspension cultures producing secondary metabolites have been permeabilized for product release by electroporation. The two cell cultures studied, i.e. Thalictrum rugosum and Chenopodium rubrum, require about 5 and 10 kV cm(-1), respectively, for complete permeabilization (release of all the intracellularly stored product). The number of electrical pulses and capacitance used had a relatively limited effect on product release while the viability of the cells was strongly influenced by the latter. Conditions for complete product release resulted in total loss of viability of the cells after treatment. The release of product from immobilized cells was also achieved by electroporation. Cells entrapped in alginate required less voltage for permeabilization than free or agarose entrapped cells.
Institute of Biotechnology, Swiss Federal Institute of Technology, Hoenggerberg, CH-8093, Zürich, Switzerland.
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PE
Brodelius
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C
Funk
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Shillito