Structural characterization of enzymatically synthesized dextran and oligosaccharides from Leuconostoc mesenteroides NRRL B-1426 dextransucrase.
Leuconostoc mesenteroides NRRL B-1426 dextransucrase synthesized a high molecular mass dextran (>2 × 10(6) Da) with ~85.5% α-(1→6) linear and ~14.5% α-(1→3) branched linkages. This high molecular mass dextran containing branched α-(1→3) linkages can be readily hydrolyzed for the production of enzyme-resistant isomalto-oligosaccharides. The acceptor specificity of dextransucrase for the transglycosylation reaction was studied using sixteen different acceptors. Among the sixteen acceptors used, isomaltose was found to be the best, having 89% efficiency followed by gentiobiose (64%), glucose (30%), cellobiose (25%), lactose (22.5%), melibiose (17%), and trehalose (2.3%) with reference to maltose, a known best acceptor. The β-linked disaccharide, gentiobiose, showed significant efficiency for oligosaccharide production that can be used as a potential prebiotic.
Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India. arungoyl@iitg.ernet.in.
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Kothari
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Goyal
D003911:Dextrans / Q000096:biosynthesis* / Q000737:chemistry*; D005964:Glucosyltransferases / Q000378:metabolism*; D007934:Leuconostoc / Q000737:chemistry* / Q000201:enzymology*; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry*
D003911:Dextrans; D009844:Oligosaccharides; D005964:Glucosyltransferases; C020025:dextransucrase