Callus formation from leaf mesophyll protoplasts of Malus Xdomestica Borkh. cv. Greensleeves.
Large yields (1.85 × 10(7)/g.f.wt.) of viable protoplasts were obtained from leaves of axenic shoot cultures of Malus Xdomestica Borkh. cv. 'Greensleeves'. Protoplasts cultured in liquid or agarose semi-solidified KM8P medium underwent cell wall regeneration and colony formation.Protoplast-derived cell colonies developed to callus on semi-solid KM8 medium. This is the first report of callus formation from mesophyll protoplasts of apple.
Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, Nottingham, Great Britain.
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Power