Genetic mapping between Gli-B1 locus and a telomeric C-heterochromatin band in wheat.
Using C-banding it has been possible to prove that the bread wheat varieties 'Holdfast' and 'CapelleDesprez' shows an intense band of telomeric heterochromatin on the short arm of chromosome 1B, while the variety 'Pané-247' presents a very thin band. Gliadin study using pH-acid electrophoresis revealed the existence of differences in the Gli-B1 locus in the three varieties. Analysis of the progeny of the (P x H) x CD hybrid revealed recombination between the heterochromatin C-band and locus Gli-B1, and allowed the genetic distance between the two markers to be calculated as 6.55±3.16 cMorgan. This is the first time the genetic distance from a locus to the chromosome telomere has been directly obtained in wheat. The heterochromatin C-band studied here gives us a cytological marker on chromosome 1B that can be used as a reference point in the localization of other genes.
Department of Cellular Biology and Genetics, University of Alcala de Henares, Alcala de Henares, E-28871, Madrid, Spain.
J M
JM
Gonzalez
G
G
Hueros
E
E
Gomez
J C
JC
Sanz