Growth and preparation of Staphylococcus epidermidis for NMR metabolomic analysis.
The "omics" era began with transcriptomics and this progressed into proteomics. While useful, these approaches provide only circumstantial information about carbon flow, metabolic status, redox poise, etc. To more directly address these metabolic concerns, researchers have turned to the emerging field of metabolomics. In our laboratories, we frequently use NMR metabolomics to acquire a snapshot of bacterial metabolomes during stressful or transition events. Irrespective of the "omics" method of choice, the experimental outcome depends on the proper cultivation and preparation of bacterial samples. In addition, the integration of these large datasets requires that these cultivation conditions be clearly defined.
School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE, USA.
Greg A
GA
Somerville
Robert
R
Powers
D046508:Culture Techniques; D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics / Q000379:methods*; D025341:Principal Component Analysis; D013212:Staphylococcus epidermidis / Q000254:growth & development* / Q000378:metabolism