Isolation of plasma-membrane-bound calcium/calmodulin-regulated protein kinase from pea using Western blotting.
Membranes isolated from pea buds contain protein-kinase activity which is greatly activated by low concentrations of calcium ions. This paper describes a simple purification of this enzyme with a novel means of detecting enzyme activity by Western blotting. The purified enzyme appears to autophosphorylate primarily on serine residues, is activated by bovine calmodulin and additional evidence from phase partitioning indicate most of this enzyme to be located in the plasma membrane.
Botany Department, University of Edinburgh, Mayfield Road, EH9 3JH, Edinburg, UK.
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Blowers
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Hetherington
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Trewavas