Mollusc C-reactive protein crosses species barrier and reverses hepatotoxicity of lead in rodent models.
Achatina fulica C-reactive protein (ACRP) reversed the toxic effects of lead nitrate both in vivo in mice and in vitro in rat hepatocytes restoring the basal level of cell viability, lipid peroxidation, reduced glutathione and superoxides. Cytotoxicity was also significantly ameliorated in rat hepatocytes by in vitro pre-treatments with individual subunits (60, 62, 90 and 110 kDa) of ACRP. Annexin V-Cy3/CFDA dual staining showed significant reduction in the number of apoptotic hepatocytes pre-treated with ACRP. ACRP induced restoration of mitochondrial membrane potential was remarkable. ACRP pre-treatment prevented Pb-induced apoptosis mediated by caspase activation. The antagonistic effect of ACRP may be due to scavenging of reactive oxygen species which maintained the homeostasis of cellular redox potential as well as reduced glutathione status. The results suggest that ACRP crosses the species barrier and it may be utilized as a viable exogenous agent of cytoprotection against heavy metal related toxicity.
Environmental Toxicology Laboratory, Department of Zoology, Centre for Advanced Studies, Visva-Bharati University, Santiniketan 731 235, India.
Sandip
S
Mukherjee
Sarmishtha
S
Chatterjee
Shuvasree
S
Sarkar
Soumik
S
Agarwal
Rakesh
R
Kundu
Sudipta
S
Maitra
Shelley
S
Bhattacharya
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002097:C-Reactive Protein / Q000494:pharmacology*; D002470:Cell Survival; D019610:Cytoprotection / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism; D015386:Hazardous Substances / Q000633:toxicity; D022781:Hepatocytes / Q000187:drug effects* / Q000473:pathology; D007854:Lead / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000473:pathology; D008974:Mollusca; D009566:Nitrates / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism
D015386:Hazardous Substances; D009566:Nitrates; D017382:Reactive Oxygen Species; D007854:Lead; C017461:lead nitrate; D002097:C-Reactive Protein; D005978:Glutathione