Effect of yogurt containing Bifidobacterium animalis subsp . lactis DN-173010 probiotic on dental plaque and saliva in orthodontic patients.
OBJECTIVE
To assess how consumption of yogurt containing Bifidobacterium animalis subsp. lactis DN-173010 probiotic for a period of 2 weeks affects salivary and dental plaque levels of mutans streptococci and lactobacilli in patients undergoing orthodontic treatment.


METHODS
A crossover, double-blind, randomized and placebo-controlled clinical trial was performed with 26 volunteers. The study was divided into four periods. During periods 2 and 4, the volunteers ingested yogurt containing probiotic or control yogurt daily for 2 weeks. Periods 1 and 3 were a 1-week run-in period and 4-week washout period, respectively. Saliva and dental plaque samples were collected from each participant at the end of each period. Mutans streptococci, lactobacilli, and total cultivable microorganisms were counted. Values were compared between groups and across periods with the Wilcoxon's test.


RESULTS
There was no difference between the yogurt containing probiotic and the control yogurt for any of the studied variables (all p > 0.05). A reduction in counts of total cultivable microorganisms was observed in dental plaque samples after ingestion of either yogurts (both p < 0.05 vs. baseline), but not in saliva (p < 0.05).


CONCLUSIONS
Daily ingestion of yogurt with or without B. animalis subsp. lactis for a period of 2 weeks was beneficial in reducing total microbial counts in dental plaque. Therefore, no additional benefits were achieved by the use of the tested probiotic strain.
Graduate Program in Dentistry, Federal University of Pelotas, Pelotas, Brazil.
G S
GS
Pinto
M S
MS
Cenci
M S
MS
Azevedo
M
M
Epifanio
M H
MH
Jones
D000293:Adolescent; D000328:Adult; D058491:Bacterial Load; D001644:Bifidobacterium / Q000502:physiology*; D002648:Child; D018592:Cross-Over Studies; D003773:Dental Plaque / Q000382:microbiology*; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D007778:Lactobacillus / Q000502:physiology; D008297:Male; D056265:Microbial Interactions; D010919:Placebos; D019936:Probiotics / Q000627:therapeutic use*; D012463:Saliva / Q000378:metabolism / Q000382:microbiology*; D012636:Secretory Rate / Q000502:physiology; D013295:Streptococcus mutans / Q000502:physiology; D015014:Yogurt / Q000382:microbiology*; D055815:Young Adult
D010919:Placebos