Callus formation and plantlet regeneration from protoplasts derived from suspension cultures of wheat (Triticum aestivum L.).
Protoplasts were isolated from anther-derived suspension cultures of commercial wheat (Triticum aestivum L. cv. Chris). The protoplasts were released enzymatically and isolated by centrifugation on a sucrose cushion. The isolated protoplasts were initially cultured in a liquid medium in the dark. Numerous microcalli were produced under these conditions, some of which differentiated into globular embryos. Upon transfer to a solid medium and exposure to 16h/8h light/dark cycle, the protocalli proliferated and many of the somatic embryos matured. Complete plantlets were obtained and maintained in sterile culture.
Monsanto Agricultural Products Co., 700 Chesterfield Village Parkway, 63198, Chesterfield Village, MO, USA.
R
R
Harris
M
M
Wright
M
M
Byrne
J
J
Varnum
B
B
Brightwell
K
K
Schubert