Tissue, cell culture and micropropagation of Mandevilla velutina, a natural source of a bradykinin antagonist.
Leaf, stem and root explants of Mandevilla velutina were cultured in vitro and produced vigorous callus in LS basal medium containing one auxin (2,4-D or NAA) plus BAP. Calli can be subcultured indefinitely with vigorous growth. Subculture of calli to NAA (1.0 mg/l) plus BAP (5.0 mg/l) caused profuse regeneration of shoots. Isolated shoots were rooted in basal medium plus NAA (5.0 mg/l) or IBA (8.0 mg/l). Rapidly growing cell suspensions can be easily obtained from friable callus cultured in liquid medium.
Plant Tissue Culture Laboratory, Department of Botany, Institute of Biosciences, University of São Paulo, C.P. 11461, 05499, São Paulo SP, Brazil.
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Guerra