Combined electron-spin-resonance, X-ray-diffraction studies on phospholipid vesicles obtained from cold-hardened wheats : I. An attempt to correlate electron-spin-resonance spectral characteristics with frost resistance.
Phospholipid multibilayers, obtained from two cultivars of thermally acclimated wheats of different frost resistances (Triticum aestivum L. cv. Penjamo 62, the sensitive cultivar, and T. aestivum L. cv. Miranovskaja 808, the frost-resistant cultivar), were investigated using electron-spin-resonance and X-ray-diffraction techniques. The former technique revealed two breaks in the motion of the spin-labelled fatty acid 2-(14-carboxyte-tradecyl)-2-ethyl-4,4-dimethyl-3-oxazolidinyloxyl, for both cultivars (+3°C,-17° C and +5° C,-18° C, respectively) when grown at 22° C. The resistant cultivar compensated for exposure to cold (+2° C) by shifting the onset of the apparent phase-separation temperature from +3° C to-16° C. The sensitive cultivar was unable to do so. X-ray diffraction did not reveal fluid-to-gel transitions between +20° C and-10° C in any of the samples. The possible role of the formation of relatively ordered aggregates or clusters of lipid molecules discerned by spin probe within the otherwise freely dispersed liquid-like lipids is discussed in terms of freezing injury of plants.
Department of Plant Physiology, University of Groningen, Haren, The Netherlands.
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Vigh
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Horváth
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Woltjes
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Farkas
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van Hassett
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Kuiper