Gentamicin-induced structural damage of human and artificial (biomimetic) otoconia.
CONCLUSIONS
Gentamicin causes irreversible structural damage of human and artificial otoconia by progressive dissolution of calcite. The inner architecture of otoconia is strongly affected by degradation scenarios during gentamicin exposure. Artificial otoconia can be used as a model system mimicking the chemical attacks for detailed investigations.


OBJECTIVE
To investigate the chemical interactions of gentamicin with natural calcite and human and artificial otoconia under in vivo conditions.


METHODS
Pure calcite crystals and artificial and human otoconia were exposed to gentamicin injection solutions at various concentrations. Morphological changes were observed in time steps by the use of environmental scanning electron microscopy (ESEM).


RESULTS
Dissolution of pure calcite crystals results in the formation of well oriented nanoshoots indicating an irreversible chemical reaction with gentamicin. Human and artificial otoconia reveal irreversible structural changes of their surface areas as well as of their inner structure, resulting in characteristic changes at different gentamicin concentrations. Minor changes are first observed by surface alterations and dissolution of calcite in the belly region. Major changes result in further reduction of the belly area reaching the center of symmetry. Finally, a complete dissolution of the branches takes place. Artificial otoconia provide detailed insight into surface alterations.
Department of Otorhinolaryngology, Head and Neck Surgery, University Medicine Mannheim, University of Heidelberg , Mannheim , Germany.
Leif Erik
LE
Walther
Angela
A
Wenzel
Jana
J
Buder
Alexander
A
Blödow
Rüdiger
R
Kniep
D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D002119:Calcium Carbonate / Q000737:chemistry*; D003460:Crystallization; D005839:Gentamicins / Q000009:adverse effects*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D010037:Otolithic Membrane / Q000187:drug effects* / Q000648:ultrastructure*
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D002119:Calcium Carbonate