MicroRNA-490-5p inhibits proliferation of bladder cancer by targeting c-Fos.
MicroRNAs (miRNAs) are non-protein-coding sequences that play a crucial role in tumorigenesis by negatively regulating gene expression. Here, we found that miR-490-5p is down-regulated in human bladder cancer tissue and cell lines compared to normal adjacent tissue and a non-malignant cell line. To better characterize the function of miR-490-5p in bladder cancer, we over-expressed miR-490-5p in bladder cancer cell lines with chemically synthesized mimics. Enforced expression of miR-490-5p in bladder cancer cells significantly inhibited the cell proliferation via G1-phase arrest. Further studies found the decreased c-Fos expression at both mRNA and protein levels and Luciferase reporter assays demonstrated that c-Fos is a direct target of miR-490-5p in bladder cancer. These findings indicate miR-490-5p to be a novel tumor suppressor of bladder cancer cell proliferation through targeting c-Fos.
Department of Urology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Qingchun Road 79, Hangzhou 310003, Zhejiang, PR China.
Shiqi
S
Li
Xianglai
X
Xu
Xin
X
Xu
Zhenghui
Z
Hu
Jian
J
Wu
Yi
Y
Zhu
Hong
H
Chen
Yeqing
Y
Mao
Yiwei
Y
Lin
Jindan
J
Luo
Xiangyi
X
Zheng
Liping
L
Xie
D020413:3' Untranslated Regions / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D016193:G1 Phase; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D017930:Genes, Reporter; D016147:Genes, Tumor Suppressor / Q000502:physiology*; D006801:Humans; D008156:Luciferases / Q000235:genetics; D035683:MicroRNAs / Q000378:metabolism / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics*; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology*
D020413:3' Untranslated Regions; C574862:MIRN490 microRNA, human; D035683:MicroRNAs; D016760:Proto-Oncogene Proteins c-fos; D008156:Luciferases
Bladder cancer; Proliferation; c-Fos; miR-490-5p