Cutting edge: The signals for the generation of T cell memory are qualitatively different depending on TCR ligand strength.
CD8 T cell memory critically contributes to long-term immunity. Both low- and high-affinity TCR signals are able to support the differentiation of memory CD8 T cells. However, it is unclear whether the requirements for memory development change when TCR signal strength is altered. To gain further insight into this question, we used a TCRβ transmembrane domain mutant model that is defective in the generation of memory in response to high-affinity ligands. Surprisingly, lowering TCR signal strength, by stimulation with low-affinity ligands, resulted in normal memory development. Restoration of memory correlated with recovery of TCR-dependent NF-κB signaling. Thus, these data provide novel evidence that the requirements for memory are qualitatively different depending on TCR signal strength.
Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, Columbia, MO 65212;
Karin M
KM
Knudson
Sara E
SE
Hamilton
Mark A
MA
Daniels
Stephen C
SC
Jameson
Emma
E
Teixeiro
D021581:Active Transport, Cell Nucleus; D019264:Adoptive Transfer; D000818:Animals; D000941:Antigens / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D005786:Gene Expression Regulation / Q000276:immunology; D007156:Immunologic Memory / Q000276:immunology*; D018189:Immunomagnetic Separation; D008024:Ligands; D008089:Listeria monocytogenes / Q000276:immunology; D008088:Listeriosis / Q000276:immunology; D018655:Lymphocyte Count; D008231:Lymphopenia / Q000276:immunology; D038041:Lymphopoiesis; D051379:Mice; D020297:Mice, Congenic; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016328:NF-kappa B / Q000502:physiology; D010047:Ovalbumin / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D017354:Point Mutation; D017434:Protein Structure, Tertiary / Q000235:genetics; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000637:transplantation
D000941:Antigens; D008024:Ligands; D016328:NF-kappa B; D010446:Peptide Fragments; D016693:Receptors, Antigen, T-Cell, alpha-beta; D010047:Ovalbumin