Regeneration of plants from leaf explants of Cucumis melo cv. Pusa Sharbati.
Leaves of three different sizes excised from 14, 21, 28 and 35-day-old seedlings of Cucumis melo were cultured on a MS medium supplemented with a range and combination of growth regulators. Maximum shoot differentiation from the leaf explants occurred in the combined presence of BAP and 2iP at equimolar concentration of 1 μM. Regeneration potential of leaves declined with increasing size of the leaves and the age of the donor seedlings. For elongation the shoots were transferred to MS+BAP [1 μM]. Such shoots were rooted with 75% frequency on MS+IAA [0.5 μM]. The plants have been established in pots.
Department of Botany, University of Delhi, 110007, Delhi, India.
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Kathal
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Bhatnagar
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Bhojwani