[Effect of fluoride on the expression of endoplasmic reticulum chaperone in ameloblast of rat incisor].
OBJECTIVE
To investigate the effect of different concentrations of fluoride on the expression of endoplasmic reticulum chaperone, and to explore the mechanism of dental fluorosis in rat.


METHODS
Thirty Wistar rats were randomly divided into 3 groups. Immunohistochemistry was used to detect the expression of CRT, GRP78, XBP-1 and caspase-12 in rat incisors. Metamorph microscope images analysis system and SPSS 13.0 software package was used to analyze the data.


RESULTS
Typical features of dental fluorosis were found in the fluoride group. Results of immunohistochemistry showed that CRT (F=238.6, P<0.05), GRP78 (F=27.42, P<0.05), XBP-1 (F=139.7, P<0.05) and caspase-12 (F=43.91, P<0.05) were significantly different among the 3 groups.


CONCLUSIONS
Excessive fluoride can increase the secretion of CRT, GRP78, XBP-1 and caspase-12 suggest the ameloblasts and in status of endoplasmic reticulum stress and caspase-12 plays an important role during ameloblast apoptosis. Supported by National Natural Science Foundation of China (81072245) and Natural Science Foundation of Liaoning Province (20102278).
Department of Preventive Dentistry, School of Stomatology, China Medical University. Shenyang 110002, Liaoning Province, China.E-mail:zhangkaiqiang1208@gmail.com.
Kai-qiang
KQ
Zhang
Ying
Y
Zhang
Lu
L
Liu
He-feng
HF
Gu
Lin
L
Ma
D000565:Ameloblasts; D000818:Animals; D017209:Apoptosis; D004721:Endoplasmic Reticulum; D000091342:Endoplasmic Reticulum Chaperone BiP; D005459:Fluorides; D009050:Fluorosis, Dental; D006360:Heat-Shock Proteins; D007180:Incisor; D010710:Phosphates; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; D010710:Phosphates; C012980:fluorophosphate; D005459:Fluorides