Metabolic engineering of Thermoanaerobacterium saccharolyticum for n-butanol production.
The thermophilic anaerobe Thermoanaerobacterium saccharolyticum JW/SL-YS485 was investigated as a host for n-butanol production. A systematic approach was taken to demonstrate functionality of heterologous components of the clostridial n-butanol pathway via gene expression and enzymatic activity assays in this organism. Subsequently, integration of the entire pathway in the wild-type strain resulted in n-butanol production of 0.85 g/L from 10 g/L xylose, corresponding to 21% of the theoretical maximum yield. We were unable to integrate the n-butanol pathway in strains lacking the ability to produce acetate, despite the theoretical overall redox neutrality of n-butanol formation. However, integration of the n-butanol pathway in lactate deficient strains resulted in n-butanol production of 1.05 g/L from 10 g/L xylose, corresponding to 26% of the theoretical maximum.
Thayer School of Engineering, 14 Engineering Drive, Hanover, NH 03755, USA.
Ashwini
A
Bhandiwad
Mascoma Corporation, 67 Etna Rd, Lebanon, NH 03766, USA.
A Joe
AJ
Shaw
Thayer School of Engineering, 14 Engineering Drive, Hanover, NH 03755, USA.
Adam
A
Guss
Thayer School of Engineering, 14 Engineering Drive, Hanover, NH 03755, USA.
Anna
A
Guseva
Universität Rostock, Albert-Einstein-Str. 3, Rostock 18051, Germany.
Hubert
H
Bahl
Thayer School of Engineering, 14 Engineering Drive, Hanover, NH 03755, USA; Mascoma Corporation, 67 Etna Rd, Lebanon, NH 03766, USA. Electronic address: lee.r.lynd@dartmouth.edu.
Lee R
LR
Lynd
D020001:1-Butanol / Q000378:metabolism*; D060847:Metabolic Engineering; D046950:Thermoanaerobacterium / Q000235:genetics / Q000378:metabolism
D020001:1-Butanol
Biofuels; Metabolic engineering; Thermophiles; n-Butanol