Genetic disruption of protein phosphatase 5 in mice prevents high-fat diet feeding-induced weight gain.
The role of serine/threonine protein phosphatase 5 (PP5) in the development of obesity and insulin resistance associated with high-fat diet-feeding (HFD) was examined using PP5-deficient mice (Ppp5c(-/-)). Despite similar caloric intake, Ppp5c(-/-) mice on HFD gained markedly less weight and did not accumulate visceral fat compared to wild-type littermates (Ppp5c(+/+)). On a control diet, Ppp5c(-/-) mice had markedly improved glucose control compared to Ppp5c(+/+) mice, an effect diminished by HFD. However, even after 10 weeks of HFD glucose control in Ppp5c(-/-) mice was similar to that observed in Ppp5c(+/+) mice on the control diet. Thus, PP5 deficiency confers protection against HFD-induced weight gain in mice.
Department of Clinical Science and Education, Södersjukhuset, Karolinska Institutet, Stockholm, Sweden. Electronic address: ngrankvist@sbmri.org.
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Grankvist
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Honkanen
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Sjöholm
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Ortsäter
D000818:Animals; D001786:Blood Glucose; D059305:Diet, High-Fat / Q000009:adverse effects; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D009687:Nuclear Proteins / Q000172:deficiency / Q000235:genetics*; D009765:Obesity / Q000209:etiology / Q000235:genetics; D010749:Phosphoprotein Phosphatases / Q000172:deficiency / Q000235:genetics*; D015430:Weight Gain / Q000235:genetics*
D001786:Blood Glucose; D007328:Insulin; D009687:Nuclear Proteins; D010749:Phosphoprotein Phosphatases; C089641:protein phosphatase 5
Diabetes; High-fat diet; Insulin sensitivity; Knockout mouse; Obesity; Protein phosphatase 5