Regeneration of intergeneric somatic hybrid plants between Lycopersicon esculentum and Solanum muricatum.
Mesophyll protoplasts of tomato (Lycopersicon esculentum) and pepino (Solanum muricatum) were fused by using an electrofusion method and cultured in modified MS medium supplemented with naphthaleneacetic acid and kinetin, in which only pepino and somatic hybrid protoplasts could divide. Somatic hybrid plants showing intermediate characteristics in morphology were regenerated from the calli exhibiting vigorous growth in contrast with those of pepino. The hybrid nature of these plants was confirmed by cytological observation and biochemical analyses of phosphoglucomutase isozymes and the fraction-1-protein. The regenerated somatic hybrids grew to flowering stage and set fruits.
Takii Plant Breeding and Experiment Station, 1360 Hari, Kohsei, Kohka, 520-32, Shiga, Japan.
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Sakomoto
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Taguchi