Exploration of novel predictive markers in rat plasma of the early stages of chronic renal failure.
To identify blood markers for early stages of chronic kidney disease (CKD), blood samples were collected from rats with adenine-induced CKD over 28 days. Plasma samples were subjected to metabolomic profiling by liquid chromatography-mass spectrometry, followed by multivariate analyses. In addition to already-identified uremic toxins, we found that plasma concentrations of N6-succinyl adenosine, lysophosphatidylethanolamine 20:4, and glycocholic acid were altered, and that these changes during early CKD were more sensitive markers than creatinine concentration, a universal indicator of renal dysfunction. Moreover, the increase in plasma indoxyl sulfate concentration occurred earlier than increases in phenyl sulfate and p-cresol sulfate. These novel metabolites may serve as biomarkers in identifying early stage CKD.
Toshihiro
T
Kobayashi
Yuriko
Y
Matsumura
Toshihiko
T
Ozawa
Hiroyuki
H
Yanai
Atsuo
A
Iwasawa
Toshiaki
T
Kamachi
Kouichi
K
Fujiwara
Noriaki
N
Tanaka
Masahiro
M
Kohno
D000225:Adenine; D000241:Adenosine / Q000031:analogs & derivatives* / Q000097:blood; D000818:Animals; D015415:Biomarkers / Q000097:blood; D002853:Chromatography, Liquid; D003408:Cresols / Q000097:blood; D042241:Early Diagnosis; D006000:Glycocholic Acid / Q000097:blood*; D007200:Indican / Q000097:blood*; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000139:chemically induced / Q000175:diagnosis; D008246:Lysophospholipids / Q000097:blood*; D008297:Male; D055432:Metabolomics; D015999:Multivariate Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013463:Sulfuric Acid Esters / Q000097:blood; D053719:Tandem Mass Spectrometry
D015415:Biomarkers; D003408:Cresols; D008246:Lysophospholipids; D013463:Sulfuric Acid Esters; C008301:lysophosphatidylethanolamine; C043565:succinyladenosine; C408690:4-cresol sulfate; C025208:phenylsulfate; D006000:Glycocholic Acid; D000225:Adenine; D000241:Adenosine; D007200:Indican