A liquid array platform for the multiplexed analysis of synthetic molecule-protein interactions.
Synthetic molecule microarrays, consisting of many different compounds spotted onto a planar surface such as modified glass or cellulose, have proven to be useful tools for the multiplexed analysis of small molecule- and peptide-protein interactions. However, these arrays are technically difficult to manufacture and use with high reproducibility and require specialized equipment. Here we report a more convenient alternative composed of color-encoded beads that display a small molecule protein ligand on the surface. Quantitative, multiplexed assay of protein binding to up to 24 different ligands can be achieved using a common flow cytometer for the readout. This technology should be useful for evaluating hits from library screening efforts, the determination of structure activity relationships, and certain types of serological analyses.
Departments of Chemistry and Cancer Biology, The Scripps Research Institute, Scripps Florida , 130 Scripps Way, Jupiter , Florida 33458, United States.
Todd M
TM
Doran
Thomas
T
Kodadek
D004396:Coloring Agents / Q000032:analysis; D008024:Ligands; D040081:Protein Array Analysis / Q000295:instrumentation*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*
D004396:Coloring Agents; D008024:Ligands; D011506:Proteins