Somatic embryogenesis from leaf protoplasts of Rauvolfia vomitoria shoot cultures.
Rauvolfia vomitoria mesophyll protoplasts have been isolated from axenic shoot cultures and cultured (10(5)-10(6) protoplasts per ml) in Murashige and Tucker liquid medium containing growth regulators. Within 6-8 weeks, a mixed population of calli and proembryos were obtained and transferred on solid media. Calli produced shoots; however, rooting did not occur. Somatic embryos achieved different patterns of development. In particular, whole plantlets have been obtained either directly through germination of primary embryos or via embryogenic calli.
Laboratoire de Biologie Cellulaire et Biochimie Végétale, Faculté de Pharmacie, 2bis Boulevard Tonnellé, 37042, Tours cédex, France.
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Trémouillaux-Guiller
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Chénieux