In vivo NMR for ¹³C Metabolic Flux Analysis.
The use of in vivo NMR within the framework of Metabolic Flux Analysis in plants is presented. In vivo NMR allows to visualize the active metabolic network, to determine metabolic and isotopic steady state and to measure metabolic fluxes which are not necessarily accessible by isotopic steady state (stationary) Metabolic Flux Analysis. The kinetic data can be used as input for dynamic (nonstationary) Metabolic Flux Analysis. Both 1D and 2D NMR methods are employed.
Génie Enzymatique et Cellulaire, FRE CNRS 3580, Université de Picardie, Amiens Cedex, France.
Albrecht
A
Roscher
Stéphanie
S
Troufflard
Abdelghani Idrissi
AI
Taghki
D002247:Carbon Isotopes; D046508:Culture Techniques; D007700:Kinetics; D064688:Metabolic Flux Analysis; D053858:Metabolic Networks and Pathways; D019906:Nuclear Magnetic Resonance, Biomolecular; D010944:Plants / Q000737:chemistry / Q000378:metabolism; D012639:Seeds / Q000737:chemistry / Q000378:metabolism
D002247:Carbon Isotopes