Separation of mitochondria from microbodies of Pisum sativum (L. cv. Alaska) cotyledons.
A method is described for separating mitochondria from microbodies in cotyledon preparations of Pisum sativum L. cv. Alaska. Pure and intact mitochondria were obtained on a continuous: discontinuous sucrose density gradient as shown by marke-enzyme assay and electron microscopy. Manipulation of sucrose-gradient construction to widen the distance between organelles provided a quick method for the separation of the mitochondria from the microbodies. The shorter time of exposure of mitochondria to centrifugation and osmotic stress produces mitochondria free of contamination.
Department of Plant Biology, Newcastle University, NE1 7RU, Newcastle upon Tyne, UK.
N
N
Burgess
G W
GW
Beakes
D R
DR
Thomas