Phosphoproteome analysis of Lotus japonicus seeds.
In this study, we report the first dataset of phosphoproteins of the seeds of a model plant, Lotus japonicus. This dataset might be useful in studying the regulatory mechanisms of seed germination in legume plants. By proteomic analysis of seeds following water absorption, we identified a total of 721 phosphopeptides derived from 343 phosphoproteins in cotyledons, and 931 phosphopeptides from 473 phosphoproteins in hypocotyls. Kinase-specific prediction analyses revealed that different kinases were activated in cotyledons and hypocotyls. In particular, many peptides containing ATM-kinase target motifs, X-X-pS/pT-Q-X-X, were detected in cotyledons. Moreover, by real-time RT-PCR analysis, we found that expression of a homolog of ATM kinase is upregulated specifically in cotyledons, suggesting that this ATM-kinase homolog plays a significant role in cell proliferation in the cotyledons of L. japonicus seeds. The data have been deposited to the ProteomeXchange with identifier PXD000053 (http://proteomecentral.proteomexchange.org/dataset/PXD000053).
Advanced Medical Research Center, Yokohama City University, Yokohama, Japan.
Yoko
Y
Ino
Akiyo
A
Ishikawa
Ayako
A
Nomura
Hideyuki
H
Kajiwara
Kyuya
K
Harada
Hisashi
H
Hirano
D018548:Cotyledon / Q000737:chemistry; D018546:Hypocotyl / Q000737:chemistry; D000070116:Lotus / Q000737:chemistry*; D013058:Mass Spectrometry; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry; D010750:Phosphoproteins / Q000032:analysis* / Q000737:chemistry; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods; D012639:Seeds / Q000737:chemistry*
D010446:Peptide Fragments; D010750:Phosphoproteins; D010940:Plant Proteins; D020543:Proteome
Legume; Lotus japonicus; MS; Phosphoprotein; Plant proteomics