The two β-oxidation sites in pea cotyledons : Carnitine palmitoyltransferase: location and function in pea mitochondria.
Two sites for β-oxidation of fatty acids in pea (Pisum sativum L.) cotyledons exist. One site is the microbody, the other the mitochondrion. Mitochondrial β-oxidation of fatty acids is carnitine-dependent. The fatty acid permeates the membrane as palmitoylcarnitine which is formed from cytosolic-side palmitoyl-CoA by a carnitine palmitoyltransferase located on the exterior face of the inner mitochondrial membrane as a peripheral protein. A single-gated pore integral membrane translocator is proposed to exchange the palmitoylcarnitine for carnitine or acetylcarnitine across the membrane. An internal (matrix side) carnitine palmitoyltransferase then reforms palmitoyl-CoA which enters β-oxidation and subsequently the tricarboxylic-acid cycle.
Department of Plant Biology, Newcastle University, NE1 7RU, Newcastle upon Tyne, UK.
D R
DR
Thomas
C
C
Wood