Neuroprotective effect of ebselen against intracerebroventricular streptozotocin-induced neuronal apoptosis and oxidative stress in rats.
The goal of this study was to examine the neuroprotective effect of ebselen against intracerebroventricular streptozotocin (ICV-STZ)-induced oxidative stress and neuronal apoptosis in rat brain. A total of 30 adult male Sprague-Dawley rats were randomly divided into 3 groups of 10 animals each: control, ICV-STZ, and ICV-STZ treated with ebselen. The ICV-STZ group rats were injected bilaterally with ICV-STZ (3 mg/kg) on days 1 and 3, and ebselen (10 mg/kg/day) was administered for 14 days starting from 1st day of ICV-STZ injection to day 14. Rats were killed at the end of the study and brain tissues were removed for biochemical and histopathological investigation. Our results demonstrated, for the first time, the neuroprotective effect of ebselen on Alzheimer's disease (AD) model in rats. Our present study, in ICV-STZ group, showed significant increase in tissue malondialdehyde levels and significant decrease in enzymatic antioxidants superoxide dismutase and glutathione peroxidase in the frontal cortex tissue. The histopathological studies in the brain of rats also supported that ebselen markedly reduced the ICV-STZ-induced histopathological changes and well preserved the normal histological architecture of the frontal cortex tissue. The number of apoptotic neurons was increased in frontal cortex tissue after ICV-STZ administration. Treatment of ebselen markedly reduced the number of degenerating apoptotic neurons. The study demonstrates the effectiveness of ebselen, as a powerful antioxidant, in preventing the oxidative damage and morphological changes caused by ICV-STZ in rats. Thus, ebselen may have a therapeutic value for the treatment of AD.
Department of Psychiatry, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey drcunsal@gmail.com.
Cuneyt
C
Unsal
Department of Internal Medicine, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey.
Mustafa
M
Oran
Department of Psychiatry, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey.
Yakup
Y
Albayrak
Department of Histology and Embryology, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey.
Cevat
C
Aktas
Department of Histology and Embryology, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey.
Mustafa
M
Erboga
Department of Biostatistics, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey.
Birol
B
Topcu
Department of Anatomy, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey.
Ramazan
R
Uygur
Department of Biochemistry, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey.
Feti
F
Tulubas
Department of Psychiatry, The Ministry of Health of Turkey, Marmara University Hospital Pendik Training and Research Hospital, Istanbul, Turkey.
Omer
O
Yanartas
Department of Neurosurgery, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey.
Ozkan
O
Ates
Department of Anatomy, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey.
Oguz Aslan
OA
Ozen
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001393:Azoles / Q000494:pharmacology*; D001921:Brain / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D020287:In Situ Nick-End Labeling; D054833:Isoindoles; D008297:Male; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016566:Organoselenium Compounds / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin / Q000633:toxicity
D001393:Azoles; D054833:Isoindoles; D018696:Neuroprotective Agents; D016566:Organoselenium Compounds; C042986:ebselen; D013311:Streptozocin
Intracerebroventricular streptozotocin; ebselen; neuronal apoptosis; neuronal damage; oxidative stress; rats