On-line speciation of arsenical compounds in fish and mussel extracts by HPLC-ICP-MS.
The separation and determination of sub-μmol/l levels of six environmentally significant As compounds was accomplished by means of high performance liquid chromatography (HPLC) combined online with inductively coupled plasma mass spectrometry (ICP-MS). The species of interest (arsenite and arsenate, monomethylarsonic acid, dimethylarsinic acid, arsenobetaine and arsenocholine) were quantified in fish and mussel extracts after separation on a Dionex AS7 column equipped with an on-guard AG7 column using a bicarbonate buffer as the mobile phase in a gradient mode. The species thus eluted were directly forwarded to the ICP-MS detector. The detection power of the overall system allows each As form to be determined at concentrations as low a 0.0013-0.0027 μmol 1(-1).
Istituto Superiore di Sanità, Viale Regina Elena 299, 1-00161, Rome, Italy.
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Caroli
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La Torre
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Petrucci
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Violante