[Effect of insulin on peroxiredoxin-6 in the osteogenic differentiation of rat's mandiular bone marrow stromal cells in high glucose].
OBJECTIVE
To explore the effect of insulin on the expression of peroxiredoxin-6 in osteogenic differentiation of rat's mandibular bone marrow stromal cells(rBMSCs) in high glucose.


METHODS
Bone marrow stromal cells were obtained from the mandible of Wistar rats and stimulated in three glucose concentrations mineral medium(5.5 mmol/L, 25 mmol/L and 45 mmol/L) with or without insulin(10-5mol/L) for 1, 3, 7, 14, and 21 days. The expression of prohibitin was quantified via enzyme-linked immuno sorbent assays (ELISA). The mineralization nodules were assessed at day 21 by alizarine red staining. Statistical analysis was performed using SPSS 15.0 soft ware package.


RESULTS
High glucose of 45 mmol/L inhibited mineralization of rBMSCs and insulin can improve the mineralization in high glucose. The expression of peroxiredoxin-6 in 45 mmol/L group decreased significantly compared with 5.5 mmol/L group and 25 mmol/L group. The expression of peroxiredoxin-6 in each group achieved maximum at day 21. Insulin (10-5 mol/L) increased the expression of peroxiredoxin-6 in 25 mmol/L group and 45 mmol/L group in osteogenic differentiation of rBMSCs.


CONCLUSIONS
High glucose inhibits the expression of peroxiredoxin-6 in osteogenic differentiation of rBMSCs, while insulin upregulates the expression of peroxiredoxin-6 in rBMSCs. Peroxiredoxin-6 may play an important part in later stage in osteogenic differentiation of rBMSCs.
Institute of Stomatology, General Hospital of Chinese PLA. Beijing 100853;China.E-mail:wbhwjc527@126.com.
Jun-cheng
JC
Wang
Ying
Y
Li
Dong-sheng
DS
Wang
Ling-ling
LL
E
Hong-chen
HC
Liu
D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002478:Cells, Cultured; D005947:Glucose; D007328:Insulin; D059630:Mesenchymal Stem Cells; D010012:Osteogenesis; D054465:Peroxiredoxin VI; D051381:Rats; D017208:Rats, Wistar
D007328:Insulin; D054465:Peroxiredoxin VI; C515984:Prdx6 protein, rat; D005947:Glucose