[Association between vitamin D receptor gene polymorphisms and pediatric Crohn's disease in China: a study based on gene sequencing].
OBJECTIVE
To investigate the association between TaqI, BsmI, and ApaI polymorphisms of vitamin D receptor (VDR) gene and pediatric Crohn's disease (CD) in China.


METHODS
Nineteen children with CD were selected as a case group, and 122 healthy children who underwent physical examination were selected as a control group. Serum 25-hydroxyvitamin D3 [25(OH)D3] levels were measured using ELISA. The TaqI, BsmI, and ApaI polymorphisms of VDR gene were determined by gene sequencing, and the two groups were compared in terms of genotype and allele frequencies.


RESULTS
The case group had significantly lower serum 25(OH)D3 levels than the control group (17.3±2.4 ng/mL vs 26.9±2.1 ng/mL; P<0.05). There were no significant differences in the frequencies of genotypes and alleles of TaqI, BsmI, and ApaI polymorphisms between the case and control groups (P>0.05).


CONCLUSIONS
Children with CD have low serum 25(OH)D3 levels. TaqI, BsmI, and ApaI polymorphisms of VDR gene may not be associated with susceptibility to CD among the Chinese population.
Department of Gastroenterology, Children's Hospital of Zhejiang University, School of Medicine, Hangzhou 310003, China. hzcjie@zju.edu.cn.
You-You
YY
Luo
Xiao-Li
XL
Shu
Hong
H
Zhao
Jin-Dan
JD
Yu
Ming
M
Ma
Jie
J
Chen
D000293:Adolescent; D002112:Calcifediol / Q000097:blood; D002648:Child; D002675:Child, Preschool; D003424:Crohn Disease / Q000097:blood / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide; D018167:Receptors, Calcitriol / Q000235:genetics*; D017422:Sequence Analysis, DNA
D018167:Receptors, Calcitriol; D002112:Calcifediol