Measurement of the binding of DNA to liposomes by resonance energy transfer.
A rapid and simple semiquantitative method for monitoring the attachment of DNA to liposomes is described. This technique is based on the measurement of resonance energy transfer between a membrane-embedded fluorescent pyrene lipid donor and DNA labeled with adriamycin as an acceptor.
Department of Medical Chemistry, University of Helsinki, P.O.B. 8, FIN-00014, Finland.
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Kinnunen