Cardioprotective effect of methanolic extract of Marrubium vulgare L. on isoproterenol-induced acute myocardial infarction in rats.
Isoproterenol injection (100 mg/kg; sc) produced changes in ECG pattern including ST-segment elevation and suppressed R-amplitude. The methanolic extract of M. vulgare at doses of 10, 20, and 40 mg/kg significantly amended the ECG changes. A severe myocardial necrosis and edematous along with a sharp reduction in the arterial blood pressure, left ventricular contractility (LVdP/dt(max or min)), but a marked increase in the left ventricular end-diastolic pressure (LVEDP) were seen in the isoproterenol group. All parameters were significantly improved by the extract treatment. The extract (10 mg/kg) strongly increased LVdP/dt(max). Similarly, treatment with 40 mg/kg of M. vulgare lowered the elevated LVEDP and the heart to body weight ratio. In addition to in vitro antioxidant activity, the extract suppressed markedly the elevation of malondialdehyde levels both in serum and in myocardium. The results demonstrate that M. vulgare protects myocardium against isoproterenol-induced acute myocardial infarction and suggest that the effects could be related to antioxidant activities.
Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Keyvan
K
Yousefi
Hamid
H
Soraya
Fatemeh
F
Fathiazad
Arash
A
Khorrami
Sanaz
S
Hamedeyazdan
Nasrin
N
Maleki-Dizaji
Alireza
A
Garjani
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002316:Cardiotonic Agents / Q000494:pharmacology*; D004562:Electrocardiography; D006321:Heart / Q000187:drug effects* / Q000503:physiopathology; D007545:Isoproterenol / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D027522:Marrubium / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry*; D009203:Myocardial Infarction / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D002316:Cardiotonic Agents; D008315:Malondialdehyde; D007545:Isoproterenol; D000432:Methanol