Antimicrobial activity of heterotrophic bacterial communities from the marine sponge Erylus discophorus (Astrophorida, Geodiidae).
Heterotrophic bacteria associated with two specimens of the marine sponge Erylus discophorus were screened for their capacity to produce bioactive compounds against a panel of human pathogens (Staphylococcus aureus wild type and methicillin-resistant S. aureus (MRSA), Bacillus subtilis, Pseudomonas aeruginosa, Acinetobacter baumanii, Candida albicans and Aspergillus fumigatus), fish pathogen (Aliivibrio fischeri) and environmentally relevant bacteria (Vibrio harveyi). The sponges were collected in Berlengas Islands, Portugal. Of the 212 isolated heterotrophic bacteria belonging to Alpha- and Gammaproteobacteria, Actinobacteria and Firmicutes, 31% produced antimicrobial metabolites. Bioactivity was found against both Gram positive and Gram negative and clinically and environmentally relevant target microorganisms. Bioactivity was found mainly against B. subtilis and some bioactivity against S. aureus MRSA, V. harveyi and A. fisheri. No antifungal activity was detected. The three most bioactive genera were Pseudovibrio (47.0%), Vibrio (22.7%) and Bacillus (7.6%). Other less bioactive genera were Labrenzia, Acinetobacter, Microbulbifer, Pseudomonas, Gordonia, Microbacterium, Micrococcus and Mycobacterium, Paenibacillus and Staphylococcus. The search of polyketide I synthases (PKS-I) and nonribosomal peptide synthetases (NRPSs) genes in 59 of the bioactive bacteria suggested the presence of PKS-I in 12 strains, NRPS in 3 strains and both genes in 3 strains. Our results show the potential of the bacterial community associated with Erylus discophorus sponges as producers of bioactive compounds.
Department of Biology, Faculty of Sciences, University of Porto, Porto, Portugal ; Interdisciplinary Centre of Marine and Environmental Research (CIMAR/CIIMAR), Porto, Portugal.
Ana Patrícia
AP
Graça
Joana
J
Bondoso
Helena
H
Gaspar
Joana R
JR
Xavier
Maria Cândida
MC
Monteiro
Mercedes
M
de la Cruz
Daniel
D
Oves-Costales
Francisca
F
Vicente
Olga Maria
OM
Lage
D048908:Aliivibrio fischeri / Q000187:drug effects; D020561:Alphaproteobacteria / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000898:Antibiosis; D001412:Bacillus subtilis / Q000187:drug effects; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D064307:Microbiota; D011161:Porifera / Q000382:microbiology*; D014733:Vibrio / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*
D000900:Anti-Bacterial Agents