[Bulky DNA adducts in human sperm correlate with semen parameters and sperm apoptosis].
OBJECTIVE
To explore the correlation of sperm polycyclic aromatic hydrocarbons (PAH)-DNA adducts with semen quality and sperm apoptosis.


METHODS
We collected semen samples from 433 infertile Chinese men, detected sperm PAH-DNA adducts using immunofluorescence staining and flow cytometry, and determined the rate of sperm apoptosis by TUNEL.


RESULTS
Multiple linear regression analysis showed that the level of sperm PAH-DNA adducts was correlated negatively with sperm concentration (beta = -0.632), total sperm count (beta = -0.830) and sperm motility (beta = -9.647), but positively with the rate of sperm apoptosis (beta = 0.130).


CONCLUSIONS
Sperm PAH-DNA adducts are evidently correlated with semen quality and sperm apoptosis, and play an important role in the evaluation of male productivity.
Nanjing Research Institute of Environmental Science, Nanjing, Jiangsu 210042, China. jgx@nies.org
Gui-Xiang
GX
Ji
Sheng-Min
SM
Wu
Ji-Ning
JN
Liu
Li-Li
LL
Shi
D000328:Adult; D017209:Apoptosis; D018736:DNA Adducts / Q000032:analysis*; D006801:Humans; D007248:Infertility, Male / Q000473:pathology* / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D012661:Semen / Q000737:chemistry*; D055101:Semen Analysis; D013076:Sperm Count; D013094:Spermatozoa; D055815:Young Adult
D018736:DNA Adducts; D011084:Polycyclic Aromatic Hydrocarbons; C095911:polycyclic aromatic hydrocarbons-DNA adduct