Identification of a differential expression signature associated with tumorigenesis and metastasis of laryngeal carcinoma.
OBJECTIVE
Metastasis is the most significant prognostic factor for laryngeal carcinoma which necessitates the identification of molecular alterations associated with metastasis. The identification of such molecular alterations will not only prove useful in treatment but also provide insight into mechanisms of cancer metastasis. The studies conducted so far have not specifically focused on metastasis or invasion pathways. Therefore we investigated the expression profiles with a pathway focused approach.


METHODS
Total RNA was extracted from 36 laryngeal tumors and paired cancer free tissue. Expression levels of 88 genes were determined using a PCR array system following cDNA synthesis. Obtained data was used for the calculation of altered expression levels, facilitating relevant algorithms. Significant alterations were determined according to their p-value obtained by Student's t-test.


RESULTS
Sixteen genes have shown altered expression when compared with adjacent cancer-free tissue. 2 of these 16 genes have shown differential expression in tumors with neck metastasis in respect to non-metastatic tumors.


CONCLUSIONS
We found that TGFB1, TIMP1, c-Myc, SPARC, COL4A2 and SOX4 show altered expression in laryngeal tumors. c-Myc and SOX4 expression is decreased as laryngeal tumors switch to metastatic phenotype.
Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey. Electronic address: ecoskunpinar@gmail.com.
E
E
Coskunpinar
Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey. Electronic address: yaseminmusteri@yahoo.com.
Y M
YM
Oltulu
Department of ORL and Head & Neck Surgery, Istanbul Faculty of Medicine, Istanbul, Turkey. Electronic address: ksorhan@yahoo.com.
K S
KS
Orhan
Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey. Electronic address: otiryakioglu@gmail.com.
N O
NO
Tiryakioglu
Department of ORL and Head & Neck Surgery, Istanbul Faculty of Medicine, Istanbul, Turkey. Electronic address: drdeniz.ent@gmail.com.
D
D
Kanliada
Department of Medical Biology, Faculty of Medicine at Bezmialem University, Istanbul, Turkey. Electronic address: fakbas@bezmialem.edu.tr.
F
F
Akbas
D063646:Carcinogenesis / Q000235:genetics* / Q000473:pathology; D018076:DNA, Complementary / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D012313:RNA / Q000235:genetics
D018076:DNA, Complementary; D004273:DNA, Neoplasm; D012313:RNA
COL4A2; FDR; GSEA; HPV; Human papillomavirus; LSCC; PCR; RNA; SOX4; SPARC; SRY-related HMG-box-4; TGFB1; TIMP1; Tissue Inhibitor of Metalloproteinase I; Transforming Growth Factor Beta I; c-Myc; cDNA; collagen alpha-2(IV); complementary deoxyribonucleic acid; false discovery rate; gene set enrichment analysis; laryngeal squamous cell carcinoma; polymerase chain reaction; qPCR; quantitative polymerase chain reaction; ribonucleic acid; secreted protein acidic and rich in cysteine; v-myc avian myelocytomatosis viral oncogene homolog