Diphenylhexatriene-phosphatidylcholine fluorescence in POPC vesicles: Comparison of the exponential-series and the maximum-entropy methods.
We have investigated the time-resolved fluorescence of diphenylhexatriene (DPH) covalently linked to phosphatidylcholine (PC) in palmitoyloleoylglycerophosphocholine (POPC) vesicles with special consideration of the comparison of two methods for distributional lifetime analysis: the exponential-series method (ESM) and the maximum-entropy method (MEM). Generally, both methods were found to reveal equivalent results on high-quality data. Different are the shapes of the recovered distributions (symmetry and width) as well as the time effort for the numerical analysis.
Institute of Physical Chemistry, University Wien, Währingerstraße 42, A-1090, Wien, Austria.
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A
Kungl
D
D
Anrather
G
G
Landl
H
H
Kauffmann
M
M
Auer
A
A
Hermetter
E
E
Prenner