Polyphenol oxidase activity and antioxidant properties of Yomra apple (Malus communis L.) from Turkey.
In this study, firstly, antioxidant and polyphenol oxidase (PPO) properties of Yomra apple were investigated. Seventeen phenolic constituents were measured by reverse phase-high-performance liquid chromatography (RP-HPLC). Total phenolic compounds (TPCs), ferric reducing antioxidant power (FRAP) and 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging activities were performed to measure antioxidant capacity. Some kinetic parameters (Km, Vmax), and inhibition behaviors against five different substrates were measured in the crude extract. Catechin and chlorogenic acid were found as the major components in the methanolic extract, while ferulic acid, caffeic acid, p-hydroxybenzoic acid, quercetin and p-coumaric acid were small quantities. Km values ranged from 0.70 to 10.10 mM in the substrates, and also 3-(4-hydroxyphenyl) propanoic acid (HPPA) and L-DOPA showed the highest affinity. The inhibition constant of Ki were ranged from 0.05 to 14.90 mM against sodium metabisulphite, ascorbic acid, sodium azide and benzoic acid, while ascorbic acid and sodium metabisulphite were the best inhibitors.
Department of Chemistry, Faculty of Science, Karadeniz Technical University , Trabzon , Turkey .
Zehra
Z
Can
Barbaros
B
Dincer
Huseyin
H
Sahin
Nimet
N
Baltas
Oktay
O
Yildiz
Sevgi
S
Kolayli
D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D001205:Ascorbic Acid / Q000737:chemistry; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors / Q000737:chemistry; D002109:Caffeic Acids / Q000737:chemistry / Q000302:isolation & purification; D002392:Catechin / Q000737:chemistry* / Q000302:isolation & purification; D004156:Catechol Oxidase / Q000737:chemistry* / Q000302:isolation & purification; D002726:Chlorogenic Acid / Q000737:chemistry* / Q000302:isolation & purification; D003373:Coumaric Acids / Q000737:chemistry / Q000302:isolation & purification; D004791:Enzyme Inhibitors / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D007700:Kinetics; D007980:Levodopa / Q000737:chemistry / Q000302:isolation & purification; D027845:Malus / Q000737:chemistry*; D010084:Oxidation-Reduction; D010226:Parabens / Q000737:chemistry / Q000302:isolation & purification; D010666:Phenylpropionates / Q000737:chemistry / Q000302:isolation & purification; D010851:Picrates / Q000037:antagonists & inhibitors / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D059808:Polyphenols / Q000737:chemistry* / Q000302:isolation & purification; D011422:Propionates; D011794:Quercetin / Q000737:chemistry / Q000302:isolation & purification; D013447:Sulfites / Q000737:chemistry
D000975:Antioxidants; D001713:Biphenyl Compounds; D002109:Caffeic Acids; D003373:Coumaric Acids; D004791:Enzyme Inhibitors; D010226:Parabens; D010666:Phenylpropionates; D010851:Picrates; D010936:Plant Extracts; D010940:Plant Proteins; D059808:Polyphenols; D011422:Propionates; D013447:Sulfites; D002726:Chlorogenic Acid; D007980:Levodopa; C005200:sodium metabisulfite; C008869:phloretic acid; D002392:Catechin; D011794:Quercetin; C004999:ferulic acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D004156:Catechol Oxidase; C495469:p-coumaric acid; C038193:4-hydroxybenzoic acid; D001205:Ascorbic Acid; C040048:caffeic acid
Antioxidant; Malus communis L.; Yomra apple; phenolics; polyphenoloxidase