Proteomic analysis of knock-down HLA-G in invasion of human trophoblast cell line JEG-3.
Previous studies showed that aberrant HLA-G expression in trophoblast cells plays important roles in trophoblast invasion; however, the mechanisms remain to be explored. In this study, we found that suppressed HLA-G expression could dramatically decrease the mRNA and protein expression levels of matrix metalloproteinase 2 and matrix metalloproteinase 9, and in the proteome assay, there were 3 identified proteins namely, prefoldin 1, eukaryotic translation elongation factor 2 and malate dehydrogenase 2, which were verified by Western blot and known to be associated with invasion, cell cycle and cell metabolism, respectively. Collectively, our study indicated a potential involvement of HLA-G in autocrine networks that may regulate prefoldin, MMPs and trophoblast invasion at the maternal-fetal interface in human pregnancy.
Obstetrics and Gynecology Hospital, Fudan University Shanghai 200012, China.
Haiyan
H
Liu
Xueyuan
X
Liu
Hong
H
Jin
Fengying
F
Yang
Weirong
W
Gu
Xiaotian
X
Li
D019898:Autocrine Communication; D015153:Blotting, Western; D002460:Cell Line; D002465:Cell Movement; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism; D005260:Female; D059951:HLA-G Antigens / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008291:Malate Dehydrogenase / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D018832:Molecular Chaperones / Q000378:metabolism; D011247:Pregnancy; D040901:Proteomics / Q000379:methods; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014162:Transfection; D014327:Trophoblasts / Q000378:metabolism*
D015852:Eukaryotic Initiation Factor-2; D059951:HLA-G Antigens; D018832:Molecular Chaperones; D012333:RNA, Messenger; C112806:prefoldin; D008291:Malate Dehydrogenase; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
HLA-G; MMPs; invasion; proteomics; trophoblast