UVC inhibits collagen biosynthesis through up-regulation of NF-κB p65 signaling in cultured fibroblasts.
The effects of UVC on collagen biosynthesis, prolidase activity, expression of α₂β₁ integrin, IGF-I receptor, FAK, MAP-kinases (ERK1 and ERK2) and the transcription factor NF-κB p65 were evaluated in human dermal fibroblasts. Confluent fibroblasts were treated with UVC light at a rates of 30 and 60 J/m(2). It was found that UVC-dependent decrease in collagen biosynthesis was not accompanied by parallel decrease in prolidase activity and expression. Since insulin-like growth factor receptor (IGF-IR) and α₂β₁ integrin signaling are the most potent regulators of collagen biosynthesis, the effect of UVC on IGF-IR and α₂β₁ integrin receptor expressions were evaluated. It was found that the exposure of the cells to UVC contributed to decrease in α₂β₁ integrin receptor and FAK expression and to an increase in IGF-IR and pERK1, pERK2 expressions. It was accompanied by an increase in the expression of NF-κB p65, the known inhibitor of collagen gene expression. The data suggest that UVC-dependent decrease of collagen biosynthesis in cultured human skin fibroblasts results from decrease in α₂β₁ integrin receptor signaling and activation of NF-κB p65, that is responsible for down-regulation of collagen gene expression.
Department of Medicinal Chemistry, Medical University of Bialystok, Mickiewicza 2 D, 15-222 Bialystok, Poland.
Lukasz
L
Szoka
Ewa
E
Karna
Jerzy A
JA
Palka
D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D015536:Down-Regulation; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism; D006801:Humans; D038982:Integrin alpha2beta1 / Q000378:metabolism; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D017526:Receptor, IGF Type 1 / Q000378:metabolism; D051996:Transcription Factor RelA / Q000378:metabolism*; D014466:Ultraviolet Rays; D015854:Up-Regulation / Q000528:radiation effects
D038982:Integrin alpha2beta1; D051996:Transcription Factor RelA; D003094:Collagen; D017526:Receptor, IGF Type 1; D051417:Focal Adhesion Kinase 1; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3
Collagen; IGF-IR; NF-κB p65; Prolidase; UVC; α(2)β(1) Integrin