Identification of oxindole-3-acetic acid, and metabolic conversion of indole-3-acetic acid to oxindole-3-acetic acid in Pinus sylvestris seeds.
Oxindole-3-acetic acid (OxIAA) has been identified in germinating seeds of Scots pine (Pinus sylvestris) using gas chromatography-mass spectrometry. Seeds germinated for 5 d contained 2.7 ng OxIAA·g(-1) (dry weight) whereas ungerminated seeds contained 0.2 ng·g(-1). Isotopically labelled OxIAA was formed in seeds incubated with [1'-(14)C]-, [2'-(14)C]- or [(2)H5]indole-3-acetic acid.
Department of Forest Genetics and Plant Physiology, The Swedish University of Agricultural Sciences, S-901 83, Umeå, Sweden.
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Ernstsen
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Sandberg
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Lundström