C1q binding to dengue virus decreases levels of infection and inflammatory molecules transcription in THP-1 cells.
Dengue virus infection elicits a spectrum of clinical presentations ranging from asymptomatic to severe disease. The mechanisms leading to severe dengue are not known, however it has been reported that the complement system is hyper-activated in severe dengue. Screening of complement proteins demonstrated that C1q, a pattern recognition molecule, can bind directly to dengue virus envelope protein and to whole dengue virus serotype 2. Incubation of dengue virus serotype 2 with C1q prior to infection of THP-1 cells led to decreased virus infectivity and modulation of mRNA expression of immunoregulatory molecules suggesting reduced inflammatory responses.
Fondazione Ri.MED, Via Bandiera 11, 90133 Palermo, Italy; University of Pittsburgh Center for Vaccine Research, 3501 Fifth Avenue, 9th floor, Pittsburgh, PA 15261, USA. Electronic address: brd49@cvr.pitt.edu.
Bruno
B
Douradinha
University of Pittsburgh Center for Vaccine Research, 3501 Fifth Avenue, 9th floor, Pittsburgh, PA 15261, USA.
Sean P
SP
McBurney
University of Pittsburgh Center for Vaccine Research, 3501 Fifth Avenue, 9th floor, Pittsburgh, PA 15261, USA; Virology and Experimental Therapy Laboratory, Aggeu Magalhães Research Center, Fiocruz, Recife, Pernambuco 50670-420, Brazil.
Klecia M
KM
Soares de Melo
University of Pittsburgh Center for Vaccine Research, 3501 Fifth Avenue, 9th floor, Pittsburgh, PA 15261, USA.
Amanda P
AP
Smith
Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Lewis Hall, Room 3184, 700W. Olney Road, Norfolk, VA 23507, USA.
Neel K
NK
Krishna
University of Pittsburgh Center for Vaccine Research, 3501 Fifth Avenue, 9th floor, Pittsburgh, PA 15261, USA; Department of Infectious Diseases and Microbiology, University of Pittsburgh Graduate School of Public Health, 130 DeSoto Street, Pittsburgh, PA 15261, USA.
Simon M
SM
Barratt-Boyes
University of Pittsburgh Center for Vaccine Research, 3501 Fifth Avenue, 9th floor, Pittsburgh, PA 15261, USA; Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, 523 Bridgeside Point II, 450 Technology Drive, Pittsburgh, PA 15219, USA.
Jared D
JD
Evans
University of Pittsburgh Center for Vaccine Research, 3501 Fifth Avenue, 9th floor, Pittsburgh, PA 15261, USA; Department of Infectious Diseases and Microbiology, University of Pittsburgh Graduate School of Public Health, 130 DeSoto Street, Pittsburgh, PA 15261, USA.
Eduardo J M
EJ
Nascimento
University of Pittsburgh Center for Vaccine Research, 3501 Fifth Avenue, 9th floor, Pittsburgh, PA 15261, USA; Virology and Experimental Therapy Laboratory, Aggeu Magalhães Research Center, Fiocruz, Recife, Pernambuco 50670-420, Brazil; Department of Infectious Diseases and Microbiology, University of Pittsburgh Graduate School of Public Health, 130 DeSoto Street, Pittsburgh, PA 15261, USA.
Ernesto T A
ET
Marques
D051940:B7-2 Antigen / Q000235:genetics / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D015922:Complement C1q / Q000276:immunology / Q000378:metabolism*; D003715:Dengue / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000821:virology; D003716:Dengue Virus / Q000145:classification / Q000276:immunology / Q000502:physiology*; D006801:Humans; D018836:Inflammation Mediators / Q000276:immunology*; D037181:Lectins, C-Type / Q000235:genetics / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000235:genetics / Q000276:immunology; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D051940:B7-2 Antigen; D015815:Cell Adhesion Molecules; D015922:Complement C1q; D018836:Inflammation Mediators; D037181:Lectins, C-Type; D018950:Lipopolysaccharide Receptors; D011956:Receptors, Cell Surface; D014759:Viral Envelope Proteins; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
C1q; Classical complement pathway; Dengue; Dengue fever; Dengue hemorrhagic fever; Viral persistence