Selective targeting capability acquired with a protein corona adsorbed on the surface of 1,2-dioleoyl-3-trimethylammonium propane/DNA nanoparticles.
A possible turning point in drug delivery has been recently reached: the protein shell, which covers nanocarriers in vivo, can be used for targeting. Here, we show that nanoparticles can acquire a selective targeting capability with a protein corona adsorbed on the surface. We demonstrate that lipid particles made of 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) and DNA, upon interaction with human plasma components, spontaneously become coated with vitronectin that promotes efficient uptake in cancer cells expressing high levels of the vitronectin ανβ3 integrin receptor.
Department of Molecular Medicine, "Sapienza" University of Rome , Viale Regina Elena 291, 00161 Rome, Italy.
Giulio
G
Caracciolo
Francesco
F
Cardarelli
Daniela
D
Pozzi
Fabrizio
F
Salomone
Giuseppe
G
Maccari
Giuseppe
G
Bardi
Anna Laura
AL
Capriotti
Chiara
C
Cavaliere
Massimiliano
M
Papi
Aldo
A
Laganà
D000328:Adult; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry*; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000737:chemistry / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D060066:Protein Interaction Maps; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
D005229:Fatty Acids, Monounsaturated; D039302:Integrin alphaVbeta3; D008081:Liposomes; D011506:Proteins; D000644:Quaternary Ammonium Compounds; D004247:DNA; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane