Chromosomal location by F1 monosomic analysis of endosperm proteins in bread wheat : 2. Two-dimensional fractionation of gliadins.
The gliadin components from four bread wheat cultivars: Chinese Spring, Capelle Desprez, Holdfast and Pane-247 and their monosomic F1s for the chromosomes of homoeologous groups 1 and 6 have been analyzed by two-dimensional (2-pH) polyacrylamide gel electrophoresis. Chromosomal location of gliadin genes and the allelic differences were well established by analyzing the different F1 monosomic hybrids, electrophoretical patterns and differences in relative staining intensity. A new gliadin encoded by a gene located on chromosome 6B in Chinese Spring is described. The two-dimensional patterns of gliadin in the other three varieties and the chromosomal location of their genes are reported for the first time. Relationships between gliadins in the two-dimensional patterns and the traditional system for their nomenclature are discussed.
Department of Cell Biology and Genetics, University of Alcalá de Henares, Campus Universitario, E-28871 Alcalá de Henares, Madrid, Spain.
J C
JC
Sanz
G
G
Hueros
J M
JM
Gonzalez
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N
Jouve