Detection of Alternatively Spliced or Processed RNAs in Cancer Using Oligonucleotide Microarray.
Deregulation of gene expression plays a pivotal role in tumorigenesis, so the ability to detect RNA alterations is of great value in cancer diagnosis and management. DNA microarrays have been used to measure changes in mRNA or microRNA level, but less often the change of RNA isoforms. Here we appraise the utilization of microarray in detecting alternatively processed RNAs, which have alternative splice forms, retained introns, or altered 3' untranslated regions. We cover the methodology and focus on cancer studies. Recent development in parallel or deep sequencing used in transcriptome analysis is also discussed.
Department of Molecular Biology, Beckman Research Institute of the City of Hope, 1500 East Duarte Road, Duarte, CA 91010-3000, USA.
Marieta
M
Gencheva
Lixin
L
Yang
Gong-Biao
GB
Lin
Ren-Jang
RJ
Lin
D017398:Alternative Splicing; D020869:Gene Expression Profiling; D006801:Humans; D007438:Introns; D009369:Neoplasms / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis