Numerical study of lipid translocation driven by nanoporation due to multiple high-intensity, ultrashort electrical pulses.
The dynamical translocation of lipids from one leaflet to another due to membrane permeabilization driven by nanosecond, high-intensity (>100kV/cm) electrical pulses has been probed. Our simulations show that lipid molecules can translocate by diffusion through water-filled nanopores which form following high voltage application. Our focus is on multiple pulsing, and such simulations are relevant to gauge the time duration over which nanopores might remain open, and facilitate continued lipid translocations and membrane transport. Our results are indicative of a N(½) scaling with pulse number for the pore radius. These results bode well for the use of pulse trains in biomedical applications, not only due to cumulative behaviors and in reducing electric intensities and pulsing hardware, but also due to the possibility of long-lived thermo-electric physics near the membrane, and the possibility for pore coalescence.
Center for Computational Biology and Bioinformatics, College of Natural Sciences, University of Texas, 2415 Speedway, C4500, Austin, TX 78712, USA.
Viswanadham
V
Sridhara
Dept. of Electrical & Computer Engineering, Frank Reidy Center for Bio-Electrics, Old Dominion University, Norfolk, VA 23529-0246, USA. Electronic address: rjoshi@odu.edu.
Ravindra P
RP
Joshi
D001692:Biological Transport; D002463:Cell Membrane Permeability / Q000502:physiology*; D002468:Cell Physiological Phenomena; D003198:Computer Simulation; D004553:Electric Conductivity; D004574:Electromagnetic Fields; D018274:Electroporation; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D008564:Membrane Potentials; D058608:Nanopores; D010718:Phosphatidylserines / Q000737:chemistry / Q000378:metabolism*; D016062:Porosity
D008051:Lipid Bilayers; D010718:Phosphatidylserines
ATP; DPPC; Electric pulsing; Lipid; MD; Molecular Dynamics; Nanopore; PS; TMP; Translocation; adenosine triphosphate; dipalmitoylphosphatidylcholine; phosphatidylserine; transmembrane potential