Antispasmodic and antidiarrheal activities of rhizomes of Polygonatum verticillatum maneuvered predominately through activation of K⁺ channels: Components identification through TLC.
Polygonatum verticillatum has traditionally been used for various purposes. The present study was aimed to validate the antispasmodic and antidiarrheal properties of crude methanolic extract of rhizomes of P. verticillatum (PR). Isolated rabbit jejunum preparations were suspended in tissue baths to measure the isotonic responses using Power Lab data acquisition system for the antispasmodic activity of PR, while the antidiarrheal activity was conducted in vivo in mice. PR caused complete relaxation of the spontaneous contractions of isolated rabbit jejunum preparations in a dose-dependent mode. A complete inhibition was observed against low potassium (K(+); 25 mM)-induced contractions, while the plant extract partially inhibited the high K(+)(80 mM)-induced contractions. From a mechanistic point of view, the spasmolytic effect of PR against low K(+)was antagonized by glibenclamide similar to the effect of cromakalim, thus showing the presence of constituents in PR mediating spasmolytic activity predominantly through the activation of adenosine triphosphate-sensitive K(+)channels. When tested against castor oil-induced diarrhea in mice, oral administration of the plant extract manifested marked antidiarrheal activity at the doses of 500 and 1000 mg/kg similar to loperamide. This study provided a pharmacological basis for the medicinal use of PR in abdominal colic and diarrhea.
Gandhara College of Pharmacy, Gandhara University, Peshawar, Pakistan Department of Pharmacy, Abdul Wali Khan University, Mardan, Pakistan hkdr2006@gmail.com.
Haroon
H
Khan
Department of Pharmacy, University of Peshawar, Peshawar, Pakistan.
Muhammad
M
Saeed
Natural Product Research Division, Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, Karachi, Pakistan Department of Basic Medical Sciences, Faculty of Pharmacy, Gomal University, Khyber-Pakhtoonkha, Pakistan.
Anwarul-Hassan
AH
Gilani
Department of Pharmacy, University of Peshawar, Peshawar, Pakistan.
Naveed
N
Muhammad
Natural Product Research Division, Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, Karachi, Pakistan International Centre for Chemical Sciences, HEJ Research Institute of Chemistry, University of Karachi, Karachi, Pakistan.
Najeeb
N
Ur Rehman
Natural Product Research Division, Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, Karachi, Pakistan.
Malik Hassan
MH
Mehmood
International Centre for Chemical Sciences, HEJ Research Institute of Chemistry, University of Karachi, Karachi, Pakistan.
Nadeem
N
Ashraf
D000818:Animals; D000930:Antidiarrheals / Q000494:pharmacology*; D002368:Castor Oil / Q000633:toxicity; D002855:Chromatography, Thin Layer; D003967:Diarrhea / Q000139:chemically induced / Q000378:metabolism; D005260:Female; D007583:Jejunum / Q000187:drug effects / Q000378:metabolism; D054086:KATP Channels / Q000187:drug effects*; D008297:Male; D008807:Mice, Inbred BALB C; D010276:Parasympatholytics / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D031384:Polygonatum / Q000737:chemistry*; D011817:Rabbits; D027343:Rhizome / Q000737:chemistry*
D000930:Antidiarrheals; D054086:KATP Channels; D010276:Parasympatholytics; D010936:Plant Extracts; D002368:Castor Oil
Polygonatum verticillatum; activation of ATP-sensitive K+ channels; antidiarrheal; spasmolytic