Detection of cell membrane-bound CD46 using flow cytometry.
CD46 is an important regulator of the complement system by preventing unwanted deposition of the complement activation products and opsonins C3b/C4b onto self-tissue. Recently, intracellular signals mediated by CD46 activation on several distinct human cell types have demonstrated that CD46 also plays decisive roles in immuneregulation. The growing recognition of CD46 as key regulator in several vital biological processes, led to increased demand in sensitive methods for monitoring CD46 expression and changes thereof on cells and in tissues. Here we describe a method, which allows for studying CD46 expression on the surface of cells using specific antibodies in combination with fluorescence-activated cell sorting (FACS) analysis.
Division of Transplantation Immunology and Mucosal Biology, MRC Centre for Transplantation, King's College London, Guy's Hospital, London, UK.
Martin
M
Kolev
Claudia
C
Kemper
D000911:Antibodies, Monoclonal / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D016130:Immunophenotyping; D051916:Membrane Cofactor Protein / Q000276:immunology / Q000378:metabolism*; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D017452:Receptors, IgG / Q000037:antagonists & inhibitors
D000911:Antibodies, Monoclonal; D051916:Membrane Cofactor Protein; D008565:Membrane Proteins; D017452:Receptors, IgG