[Resveratrol did not prevent sevoflurane-induced neuroapoptosis in the neonatal mice brain].
BACKGROUND
In an animal model, neonatal exposure to sevoflurane induces neuroapoptosis, leading to memory deficits in adulthood. A recent study showed that resveratrol (20 mg x kg(-1)) prevent alcohol-induced cognitive deficits and neural apotosis in rat pups postnatally exposed to ethanol. We investigated if resveratorol prevent sevoflurane induced neuroapotosis.


METHODS
Six-day-old mice were divided into two groups: resveratorol and control groups. Pups were given resveratrol orally 24h and 1h before sevoflurane anesthesia. Anesthesia was maintained for 6h. After anesthesia, apotosis was evaluated by immunohistochemical staining for activated caspase-3. Western blot analysis for cleaved poly-(adenosine diphosphate-ribose) polymerase was performed to examine apotosis.


RESULTS
Neonatal exposure to sevoflurane induced severe neuroapotosis. There were no differences between control groups and resveratrol groups with regards to immunohistochemical staining and western blot analysis.


CONCLUSIONS
Resveratrol did not prevent sevoflurane-induced neuroapoptosis in the neonatal mice brain.
Department of Anesthesiology and Pain Medicine, Tokyo Medical and Dental University, Tokyo 113-8510.
Maiko
M
Satomoto
Hiroyuki
H
Itoh
Atsujiro
A
Uchida
Koshi
K
Makita
D018685:Anesthetics, Inhalation / Q000009:adverse effects*; D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000008:administration & dosage*; D017209:Apoptosis / Q000187:drug effects*; D001921:Brain / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D008738:Methyl Ethers / Q000009:adverse effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D000077185:Resveratrol; D000077149:Sevoflurane; D013267:Stilbenes / Q000008:administration & dosage*
D018685:Anesthetics, Inhalation; D000975:Antioxidants; D008738:Methyl Ethers; D013267:Stilbenes; D000077149:Sevoflurane; D000077185:Resveratrol