Enzymatic membrane reactor for full saccharification of ionic liquid-pretreated microcrystalline cellulose.
Ultrafiltration reactors based on polymeric or ceramic membranes were shown to be suitable catalytic systems for fast enzymatic saccharification of cellulose, allowing the full recovery and reuse of enzymes. By pre-treating cellulose with the IL 1-butyl-3-methylimidazolium chloride, the suitability of this substrate for enzymatic saccharification in a reactor based on polymeric ultrafiltration membranes was demonstrated, leading to 95% cellulose hydrolysis in 4h at 50°C. The filtration process gave a clear glucose solution (up to 113 mM) at constant permeate flow (24.7 L h(-1) m(-2)), allowing the enzyme to be reused for 9 operation cycles under semi-continuous operation, without any loss of enzyme activity. Under continuous operation mode and using ceramic ultrafiltration membranes at different residence times, the enzymatic reactor showed constant profiles in both the permeate flow rate and the glucose concentration, demonstrating the excellent suitability of the proposed approach for the saccharification of cellulose.
Departamento de Bioquímica y Biología Molecular B e Inmunología, Facultad de Química, Universidad de Murcia, P.O. Box 4021, E-30100 Murcia, Spain. Electronic address: plozanor@um.es.
Pedro
P
Lozano
Berenice
B
Bernal
Antonio G
AG
Jara
Marie-Pierre
MP
Belleville
D019149:Bioreactors; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D002482:Cellulose / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism*; D005947:Glucose / Q000096:biosynthesis; D006868:Hydrolysis / Q000187:drug effects; D052578:Ionic Liquids / Q000494:pharmacology*; D008567:Membranes, Artificial; D013997:Time Factors; D014462:Ultrafiltration; D001617:beta-Glucosidase / Q000378:metabolism
D004798:Enzymes; D052578:Ionic Liquids; D008567:Membranes, Artificial; D002482:Cellulose; D001617:beta-Glucosidase; D005947:Glucose; C109691:microcrystalline cellulose
Biofuels; Cellulase; Cellulose saccharification; Ionic liquids; Membrane reactor