Control of postharvest fungal pathogens by antifungal compounds from Penicillium expansum.
The fungicidal effects of secondary metabolites produced by a strain of Penicillium expansum (R82) in culture filtrate and in a double petri dish assay were tested against one isolate each of Botrytis cinerea, Colletotrichum acutatum, and Monilinia laxa and six isolates of P. expansum, revealing inhibitory activity against every pathogen tested. The characterization of volatile organic compounds released by the R82 strain was performed by solid-phase microextraction-gas chromatographic techniques, and several compounds were detected, one of them identified as phenethyl alcohol (PEA). Synthetic PEA, tested in vitro on fungal pathogens, showed strong inhibition at a concentration of 1,230 μg/ml of airspace, and mycelium appeared more sensitive than conidia; nevertheless, at the concentration naturally emitted by the fungus (0.726 ± 0.16 m g/ml), commercial PEA did not show any antifungal activity. Therefore, a combined effect between different volatile organic compounds produced collectively by R82 can be hypothesized. This aspect suggests further investigation into the possibility of exploiting R82 as a nonchemical alternative in the control of some plant pathogenic fungi.
Criof, University of Bologna, Via Gandolfi 19, 40057 Cadriano, Bologna, Italy.
Wafa
W
Rouissi
Luisa
L
Ugolini
Camilla
C
Martini
Luca
L
Lazzeri
Marta
M
Mari
D000898:Antibiosis; D000935:Antifungal Agents / Q000378:metabolism* / Q000494:pharmacology; D001203:Ascomycota / Q000187:drug effects / Q000254:growth & development*; D020171:Botrytis / Q000187:drug effects / Q000254:growth & development; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D005506:Food Contamination / Q000032:analysis; D005519:Food Preservation / Q000379:methods*; D008826:Microbial Sensitivity Tests; D025282:Mycelium / Q000187:drug effects; D010407:Penicillium / Q000378:metabolism / Q000502:physiology*; D013172:Spores, Fungal / Q000187:drug effects
D000935:Antifungal Agents