Production of canthin-6-one alkaloids by cell suspension cultures of Brucea javanica (L.) Merr.
Cell suspension cultures of Brucea javanica (L.) Merr. were used to determine culture growth characteristics and production of canthin-6-one alkaloids. The major alkaloids produced were canthin-6-one, 11-hydroxycanthin-6-one, 5-methoxycanthin-6-one and 11-methoxycanthin-6-one. Alkaloids were synthesised throughout the 36 day growth cycle of the cells with maximum amounts within the cells occurring between days 20 to 28; approximately 10% of the alkaloids were in the medium at day 24 rising to 45% at day 32.
Department of Pharmacognosy, The School of Pharmacy, University of London, 29-39 Brunswick Square, WC1N 1AX, London, UK.
K C
KC
Liu
S L
SL
Yang
M F
MF
Roberts
J D
JD
Phillipson