Conversion of [(14)C]gibberellin A 12-aldehyde to C 19- and C 20-gibberellins in a cell-free system from immature seed of Phaseolus coccineus L.
A cell-free system prepared from developing seed of runner bean (Phaseolus coccineus L.) converted [(14)C]gibberellin A12-aldehyde to several products. Thirteen of these were identified by capillary gas chromatography-mass spectrometry as gibberellin A1 (GA1), GA4, GA5, GA6, GA15, GA17, GA19, GA20, GA24, GA37, GA38, GA44 and GA53-aldehyde, all giving mass spectra with (14)C-isotope peaks. GA8 and GA28 were also identified but contained no (14)C. All the [(14)C]GA12-aldehyde metabolites, except GA15, GA24 and GA53-aldehyde, are known endogenous GAs of P. coccineus.
Department of Botany, The University, G12 8QQ, Glasgow, UK.
C G
CG
Turnbull
A
A
Crozier
L
L
Schwenen
J E
JE
Graebe