[Study on transdermal behavior of Zhitong cataplasm].
The aim of the present research was to study the transdermal absorbability of ferulic acid and imperatorin in Zhitong cataplasm. Using methanol to extract the effective components, HPLC was established to determine drug content in Zhitong cataplasm. The excised abdominal skin of nude rats was used as the permeation model Utilizing the modified Franz diffusion cell, the suitable receiving solution was elected. The contents of ferulic acid and imperatorin in Zhitong cataplasm were 455.10, 371.66 microg x g(-1), respectively. Taken 20% ethanol- PBS (pH 8) as receiving solution, ferulic acid and imperatorin could be detected maximize, with the steady-state infiltration rates of 1.29, 0.15 microg x h(-1) x cm(-2), respectively. After 24 h, their penetration quantities were 30.03, 3.31 microg x cm(-2), with the penetration rates of 41.45%, 5.60%, respectively. The residual quantities of ferulic acid and imperatorin in skin were 0.69, 2.60 microg x cm(-2), with the retention rates of 0.95%, 4.40%, respectively. The in vitro transdermal behavior of ferulic acid in Zhitong cataplasm was close to a zero-order process, with the stratum corneum playing a rate-limiting role; whereas the percutaneous behavior of imperatorin was closer to Higuchi model, where diffusion played an important role in drugs permeation.
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. xingchen_lcm@163.com
Cong-Min
CM
Liu
Jie
J
Bai
Shou-Ying
SY
Du
Yang
Y
Lu
Peng-Yue
PY
Li
D000700:Analgesics / Q000737:chemistry / Q000378:metabolism* / Q000627:therapeutic use; D000818:Animals; D003373:Coumaric Acids / Q000378:metabolism / Q000627:therapeutic use; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000378:metabolism* / Q000627:therapeutic use; D011564:Furocoumarins / Q000378:metabolism / Q000627:therapeutic use; D007700:Kinetics; D010146:Pain / Q000188:drug therapy; D051381:Rats; D012869:Skin Absorption
D000700:Analgesics; D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D011564:Furocoumarins; C004999:ferulic acid; C031534:imperatorin