Comprehensive phylogenetic diversity of [FeFe]-hydrogenase genes in termite gut microbiota.
Phylogenetic diversity of [FeFe]-hydrogenase (HydA) in termite guts was assessed by pyrosequencing PCR amplicons obtained using newly designed primers. Of 8,066 reads, 776 hydA phylotypes, defined with 97% nucleotide sequence identity, were recovered from the gut homogenates of three termite species, Hodotermopsis sjoestedti, Reticulitermes speratus, and Nasutitermes takasagoensis. The phylotype coverage was 92-98%, and the majority shared only low identity with database sequences. It was estimated that 194-745 hydA phylotypes existed in the gut of each termite species. Our results demonstrate that hydA gene diversity in the termite gut microbiota is much higher than previously estimated.
Department of Chemical Engineering, Tsinghua University.
Hao
H
Zheng
Dylan
D
Bodington
Chong
C
Zhang
Kazuhiko
K
Miyanaga
Yasunori
Y
Tanji
Yuichi
Y
Hongoh
Xin-Hui
XH
Xing
D000818:Animals; D001419:Bacteria / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D044822:Biodiversity; D041981:Gastrointestinal Tract / Q000382:microbiology; D006864:Hydrogenase / Q000235:genetics* / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000235:genetics* / Q000378:metabolism; D020049:Isoptera / Q000382:microbiology*; D064307:Microbiota; D008969:Molecular Sequence Data; D010802:Phylogeny
D001426:Bacterial Proteins; D007506:Iron-Sulfur Proteins; C118830:iron hydrogenase; D006864:Hydrogenase