miR-154 suppresses colorectal cancer cell growth and motility by targeting TLR2.
MicroRNAs play critical roles in the development and progression of colorectal cancer (CRC). miR-154 acts as a tumor suppressor in several tumors; however, its role in CRC is poorly understood. Herein, we found that miR-154 was decreased in CRC tissues and cell lines. Ectopic expression of miR-154 remarkably suppressed cell proliferation and colony formation, migration and invasion in CRC cells. The toll-like receptor 2 (TLR2) was found to be a direct target of miR-154 in CRC cells. Inhibition of TLR2 performed similar effects with miR-154 overexpression on CRC cells, and overexpression of TLR2 could significantly reverse the tumor suppressive effects of miR-154 on CRC cells. This study suggests an essential role for miR-154 in CRC.
Anus-Intestines Department, Daqing Oilfield General Hospital, Daqing, 163000, China.
Chaoguang
C
Xin
Hao
H
Zhang
Zanchao
Z
Liu
D020413:3' Untranslated Regions; D001483:Base Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D057809:HEK293 Cells; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D034622:RNA Interference; D051195:Toll-Like Receptor 2 / Q000235:genetics* / Q000378:metabolism
D020413:3' Untranslated Regions; C561122:MIRN154 microRNA, human; D035683:MicroRNAs; C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2