Detection of optical neuronal signals in the visual cortex using continuous wave near-infrared spectroscopy.
Near-infrared spectroscopy (NIRS) measures slow hemodynamic signals noninvasively to indirectly infer the neuronal activity in the brain. However, it remains a controversy on whether this optical measurement technique can detect the optical neuronal signal, which reflects the optical changes directly associated with neuronal activity, within the visual cortex of human and non-human primates. By carefully reviewing the important factors in the detection of optical neuronal signals, we aim to investigate the feasibility of performing NIRS measurements of optical neuronal signals within the visual cortex in humans. To ensure a strong optical neuronal response, a full-field circular black and white reversing checkerboard stimulus was presented, and the reversal frequency was carefully chosen. We used a homemade continuous wave (CW) NIRS system with high detection sensitivity (of the order of 0.1 pW) to record a large area of the visual cortex (approximately 6 × 14 cm(2)). EEG was simultaneously acquired with the optical signal. Based on the mathematical morphology, we adapted the filter proposed by Gratton et al. to remove the influence of arterial pulsation and facilitate the detection and elimination of unknown artifacts from the data. We obtained reliable optical neuronal signals in 77% of the participants (10 out of 13). The amplitudes (latencies) of the obtained optical neuronal signals corresponding to the 785 and 850 nm wavelengths were 0.017 ± 0.003% (94.7 ± 8.4 ms) and 0.025 ± 0.006% (99.0 ± 7.7 ms), respectively. There were no significant differences between the latencies of the N75 component of the visual evoked potential (VEP) and optical neuronal signals at either wavelength. This is the first study to report optical neuronal signals within the visual cortex in the intact human brain using a CW NIRS system. These results indicate the feasibility of measuring noninvasive optical neuronal signals using a CW NIRS system with high detection sensitivity.
Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, China; Key Laboratory of Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China.
Bailei
B
Sun
Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, China; Key Laboratory of Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China.
Lei
L
Zhang
Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, China; Key Laboratory of Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China.
Hui
H
Gong
Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, China; Key Laboratory of Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China.
Jinyan
J
Sun
Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, China; Key Laboratory of Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China. Electronic address: qluo@mail.hust.edu.cn.
Qingming
Q
Luo
D000328:Adult; D004569:Electroencephalography; D005074:Evoked Potentials, Visual / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D010775:Photic Stimulation / Q000379:methods; D012815:Signal Processing, Computer-Assisted; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D014793:Visual Cortex / Q000502:physiology*; D055815:Young Adult
NIRS; Optical neuronal signal; Visual cortex; Visual evoked potential