Design, synthesis and characterization of a hexapeptide bio-inspired by acetylcholinesterase and its interaction with pesticide dichlorvos.
This paper describes the molecular modeling design, synthesis and characterization of a new bio-inspired hexapeptide of acetylcholinesterase enzyme and its interaction with the organophosphate pesticide dichlorvos monitored by UV-Vis spectroscopy and mass spectrometry. This strategy can contribute to the development of synthetic receptors to be coupled to biosensor transducers, avoiding the issues associated with proteins such as low stability under different pH and temperature conditions and high production cost. The resulting data of this work indicate a strong interaction between the pesticide dichlorvos and the hexapeptide (NH3(+)-Glu-His-Gly-Gly-Pro-Ser-COO(-)) with a binding constant of 4.10 × 10(5) M(-1) and the formation of an adduct by covalent binding on the serine residue from the hexapeptide.
UNESP - Univ Estadual Paulista, Institute of Chemistry, Rua Prof. Francisco Degni 55, 14800-900, Araraquara, SP, Brazil. hidekoy@iq.unesp.br.
Glauco Pilon
GP
dos Santos
Bianca Ferreira
BF
da Silva
Saulo Santesso
SS
Garrido
Marcello
M
Mascini
Hideko
H
Yamanaka
D000110:Acetylcholinesterase / Q000737:chemistry* / Q000378:metabolism; D004006:Dichlorvos / Q000032:analysis* / Q000378:metabolism; D015195:Drug Design; D013058:Mass Spectrometry / Q000379:methods*; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000378:metabolism; D010575:Pesticides / Q000032:analysis* / Q000378:metabolism; D011485:Protein Binding / Q000502:physiology; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D060327:Solid-Phase Synthesis Techniques / Q000379:methods
D009842:Oligopeptides; D010575:Pesticides; D004006:Dichlorvos; D000110:Acetylcholinesterase