Style proteins of a wild tomato (Lycopersicon peruvianum) associated with expression of self-incompatibility.
The identification, isolation and aminoterminal sequencing of two S-genotype-associated proteins from style extracts of Lycopersicon peruvianum Mill. is reported. There is a high level of homology between these two sequences and with the amino-terminal sequences of other S-allele-associated glycoproteins isolated from Nicotiana alata Link et Otto. These sequences were obtained by a new high-sensitivity method of selected twodimensional gel analysis followed by electroelution and purification of proteins by inverse-gradient high-performance liquid chromatography before sequencing.
Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3052, Parkville, Vic., Australia.
S L
SL
Mau
E G
EG
Williams
A
A
Atkinson
M A
MA
Anderson
E C
EC
Cornish
B
B
Grego
R J
RJ
Simpson
A
A
Kheyr-Pour
A E
AE
Clarke