Restriction endonuclease analysis of mitochondrial DNA from sorghum with fertile and male-sterile cytoplasms.
Mitochondrial DNA from four paired (fertile and male-sterile) lines and six isocytoplasmic strains of sorghum (Sorghum bicolor (L.) Moench) were fragmented by endonucleases and their electrophoretic patterns were examined. Cytoplasmic male sterile lines differed from their male-fertile counterparts consistently. Among the isocytoplasmic strains, KS 36A (S. verticilli-florum cytoplasm), KS 38A (S. conspicum cytoplasm), and KS 39A (S. niloticum cytoplasm) showed minor differences from the other strains. Results suggest that restriction endonuclease patterns are useful in detecting differences in mitochondrial genomes.
Departments of Genetics, Biochemistry and Agronomy, Kansas State University, 66506, Manhattan, KS, USA.
S H
SH
Lee
S
S
Muthukrishnan
E L
EL
Sorensen
G H
GH
Liang