Transformed calli obtained by direct gene transfer into sunflower protoplasts.
Helianthus annuus protoplasts were transformed with the plasmid pCaMVNEO (Frommet al. 1986) conferring kanamycin resistance to plant. Transformed calli were selected with a frequency of 4 calli for 10(6) treated protoplasts. DNA was extracted from kanamycin resistant calli. Analysis of this DNA shows the presence of the NPTII gene.
Laboratoire de Physiologie Végétale Moléculaire, Université Paris-Sud, bâtiment 430, F 91405, Orsay Cedex, France.
A L
AL
Moyne
D
D
Tagu
V
V
Thor
C
C
Bergounioux
G
G
Freyssinet
P
P
Gadal