Antiangiogenic tocotrienol derivatives from Garcinia amplexicaulis.
Phytochemical investigation of a dichloromethane extract from Garcinia amplexicaulis stem bark led to the isolation of four new tocotrienols (1-4); two known tocotrienols, two triterpenes, and a xanthone were also isolated. Their structures were mainly established using NMR and MS methods. The main compounds isolated, δ-amplexichromanol (1) and γ-amplexichromanol (2), were evaluated on VEGF-induced angiogenesis using a Matrigel assay. Compounds 1 and 2 inhibited in vitro angiogenesis of VEGF-induced human primary endothelial cells in the low nanomolar range. Their capacity to inhibit VEGF-induced proliferation of endothelial cells partially explained this activity, although δ-amplexichromanol (1) also prevented adhesion and migration processes.
Laboratoire SONAS, Université d'Angers , IFR Quasav, 49100 Angers, France.
Alexis
A
Lavaud
Pascal
P
Richomme
Marc
M
Litaudon
Ramaroson
R
Andriantsitohaina
David
D
Guilet
D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002839:Chromans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D029760:Garcinia / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D009389:Neovascularization, Pathologic / Q000188:drug therapy; D009510:New Caledonia; D019906:Nuclear Magnetic Resonance, Biomolecular; D024301:Plant Bark / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D024508:Tocotrienols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology
D020533:Angiogenesis Inhibitors; D002839:Chromans; D024508:Tocotrienols; D042461:Vascular Endothelial Growth Factor A