[Anti-hepatic fibrosis effects research on serum containing extracts of Periplaneta americana in vitro].
OBJECTIVE
To study the anti-hepatic fibrosis effect of serum containing extracts of Periplaneta americana.


METHODS
The serum contained extracts of Periplaneta americana was prepared with serologic pharmacological method. MTT method was used to observe the effect of serum containing extracts from periplaneta americana on hepatic stellate cells (HSC), and Elisa method was used to detect the contents of TGF-beta1 and collagen I in supernatant.


RESULTS
Serum containing extracts I and II (15%) of Periplaneta americana had inhibitory effect on HCS (P < 0.05) after HSC were cultured with serum containing extracts of different concentration of Periolaneta americana for 24, 48 and 72 h. At 24 and 48 h, serum containing extracts I and II of Periplaneta americana decreased the content of collagen I in supernatant without significant difference (P < 0.05). Serum containing extracts I (15%, 9%, 5.4%) of Periplaneta americana could reduce generation of TGF-beta1 in supernatant for 24 h (P < 0.05). As for 48 h, only high concentration serum containing extracts I (15%) deceased the content of TGF-beta1 in supernatant. For 24 and 48 h,serum containing extracts II couldn't reduce the content of TGF-beta1 in supernatant (P < 0.05).


CONCLUSIONS
It has definite effect on anti-hepatic fibrosis with serum containing extracts of Periplaneta americana in vitro. The mechanism may be related to inhibiting HSC propagation and reducing the production of TGF-beta1.
Department of Pharmacology, College of Pharmacy and Chemistry, Dali University, Dali 671000, China. lcyyanyan@126.com
Chun-Yan
CY
Li
Yan-Jie
YJ
Chen
Shu-Nan
SN
Li
Guang-Ming
GM
Liu
Yong
Y
Lai
D000818:Animals; D002460:Cell Line; D024042:Collagen Type I / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation / Q000187:drug effects; D055166:Hepatic Stellate Cells / Q000187:drug effects / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D010526:Periplaneta / Q000737:chemistry*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D044967:Serum / Q000737:chemistry; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
D024042:Collagen Type I; D008419:Materia Medica; C508962:Tgfb1 protein, rat; D053773:Transforming Growth Factor beta1