Water-compatible molecularly imprinted microspheres in pipette tip solid-phase extraction for simultaneous determination of five fluoroquinolones in eggs.
New water-compatible molecularly imprinted microspheres were synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization using 3-(2-carboxyethylsulfanylthiocarbonyl-sulfanyl) propionic acid as a hydrophilic chain-transfer agent, and employed as the sorbent of pipet tip molecularly imprinted solid-phase extraction (PT-MISPE) for rapid extraction and screening of ofloxacin, pefloxacin, norfloxacin, ciprofloxacin, and enrofloxacin in eggs. In comparison to conventional SPE methods, the presented PT-MISPE showed special selectivity, easy operation, and accessible device without expensive SPE apparatus. The presented PT-MISPE method combined advantages of dummy molecularly imprinted polymers and pipet tip solid-phase extraction. The presented method was linear over a calibration range of 25-2500 μg/kg with the limits of detections of 0.53-1.07 μg/kg. Good recoveries (89.1-102.5%) were achieved with relative standard deviations of 2.6-4.8%.
Key Laboratory of Pharmaceutical Quality Control of Hebei Province & College of Pharmacy, Hebei University , Baoding, 071002, China.
Suting
S
Liu
Hongyuan
H
Yan
Mingyu
M
Wang
Lihui
L
Wang
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000302:isolation & purification*; D002645:Chickens; D004531:Eggs / Q000032:analysis*; D024841:Fluoroquinolones / Q000032:analysis / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis*; D008863:Microspheres; D054802:Molecular Imprinting; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*
D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D011108:Polymers