Cathepsin L1 mimotopes with adjuvant Quil A induces a Th1/Th2 immune response and confers significant protection against Fasciola hepatica infection in goats.
Thirty goats were randomly allocated in five groups of six animals each, for immunization with 1 × 10(14) phage particles of clones 11, 13, and 13 with Quil A adjuvant and wild-type M13KE phage at the beginning and 4 weeks later. The control group received phosphate-buffered saline. All groups were challenged with 200 metacercariae at week 6 and slaughtered 14 weeks later. The mean worm burdens after challenge were reduced by 46.91% and 79.53% in goats vaccinated with clones 13 and 13 with Quil A (P < 0.05), respectively; no effect was observed in animals immunized with clone 11 and M13KE phage. Animals receiving clones 11, 13, and 13 with Quil A showed a significant reduction in eggs output. Vaccinated animals produced parasite-specific total IgG antibody which were boosted after challenge with metacercariae of F. hepatica. Furthermore, levels of anti-phage total IgG increased rapidly within 2 weeks of the first vaccination and were always significantly higher in all vaccinated goats than in the infected control group. The fluke burden of goats immunized with clones 13 and 13 with Quil A was significantly correlated with IgG2 and total IgG. Goats vaccinated with phage clones produced significantly high titres of IgG1 and IgG2 antibodies indicating a mixed Th1/Th2 response. These data indicate that cathepsin L1 mimotopes has a potential as a vaccine candidate against Fasciola hepatica, whose efficacy will be evaluated in other host species, including those of veterinary importance.
Facultad de Medicina Veterinaria y Zootecnia, Benemérita Universidad Autónoma de Puebla, 4 Sur 304 Col. Centro, CP 75482, Tecamachalco Puebla, Mexico, abel.villa@gmail.com.
Abel
A
Villa-Mancera
Alejandro
A
Reynoso-Palomar
Fernando
F
Utrera-Quintana
Lorenzo
L
Carreón-Luna
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D056668:Cathepsin L / Q000494:pharmacology*; D005210:Fasciola hepatica; D005211:Fascioliasis / Q000276:immunology / Q000517:prevention & control* / Q000662:veterinary; D006041:Goats / Q000276:immunology* / Q000469:parasitology; D056724:Immunity, Humoral; D007074:Immunoglobulin G / Q000276:immunology; D008969:Molecular Sequence Data; D010270:Parasite Egg Count; D019151:Peptide Library; D062605:Quillaja Saponins; D012503:Saponins / Q000494:pharmacology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014612:Vaccines / Q000276:immunology / Q000627:therapeutic use*
D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D019151:Peptide Library; D062605:Quillaja Saponins; D012503:Saponins; D014612:Vaccines; C046386:Quil A; D056668:Cathepsin L