Overview of current standpoints in profiling of circulating tumor cells.
The goal of this review is summarizing current technologies developed as the in vitro prognostic/diagnostic systems that can rapidly separate and detect circulating tumor cells (CTCs) from cancer patient's blood (1-10 CTCs of 1 billion red blood cells) by labeled and non-labeled method. The review is focused on three major areas of CTC research (1) Summary of previous research on capturing of CTCs, (2) New development of the in vitro prognostic diagnosis system of cancer that is capable of rapid separation of CTCs, (3) Future direction on development of new technologies for CTC profiling. Current CTC researches have helped on identifying patients who may benefit from chemotherapy before treatment, patients who may benefit from continued chemotherapy, and leading to clinical development of CTC-guided chemotherapy strategies. We analyze the feasibility of clinical application of these current CTC research for the ultimate goal of increasing the survivability of cancer patient. The biomolecular assays of viable CTCs from cancer patient may elucidate the mechanism of metastasis and tumor initiating cells and also may have high impact on the development of personalized medicine to overcome the incurable diseases.
Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.
Kyobum
K
Kim
Kwan Hyi
KH
Lee
Jongmin
J
Lee
Jonghoon
J
Choi
D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D002469:Cell Separation; D020869:Gene Expression Profiling; D006801:Humans; D025202:Molecular Diagnostic Techniques; D050997:Nanomedicine / Q000379:methods*; D009369:Neoplasms / Q000097:blood* / Q000235:genetics / Q000473:pathology; D009360:Neoplastic Cells, Circulating / Q000378:metabolism* / Q000473:pathology; D018579:Patient Selection; D010641:Phenotype; D057285:Precision Medicine; D011237:Predictive Value of Tests; D011379:Prognosis; D040081:Protein Array Analysis; D040901:Proteomics
D014408:Biomarkers, Tumor