An integral probe for capillary zone electrophoresis/continuous-flow fast atom bombardment mass spectrometry.
An integral probe for capillary zone electrophoresis/continuous-flow fast atom bombardment mass spectrometry was constructed and operated in either the coaxial or liquid-junction interface mode. Results using these interfaces for the analyses of synthetic peptides are presented. The coaxial arrangement attains the best electrophoretic performance, generally providing a greater number of theoretical plates. However, in that the electrophoresis times are generally greater for the liquid-junction interface because there is no mechanical flow resulting from the source vacuum, the overall separation efficiency of the liquid-junction interface is equal to or greater than that of the coaxial interface. In addition, the liquid-junction interface is easier to set up and operate, and allows larger inner diameter capillaries to be used to achieve higher sample loads.
The Analytical Chemistry Center and Department of Biochemistry and Molecular Biology, University of Texas Medical School, 77030, Houston, TX.
M J
MJ
Suter
R M
RM
Caprioli