A plant lipid and the platelet-activating factor stimulate ATP-dependent H(+) transport in isolated plant membrane vesicles.
A plant lipid was isolated from zucchini (Cucurbita pepo L.) membranes and from soybean (Glycine max [L.] Merr) phospholipids by thinlayer chromatography and further purified by high-performance liquid chromatography. This plant lipid was chromatographically very similar to the platelet-activating factor, an ether phospho-lipid with hormone-like properties found in mammals. Both the plant lipid and the platelet-activating factor stimulated ATP-dependent H(+) transport in isolated membrane vesicles from zucchini hypocotyls.
Botanisches Institut, Universität Bonn, Venusbergweg 22, D-5300, Bonn 1, Federal Republic of Germany.
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GF
Scherer
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Stoffel