Biosynthesis of osmoregulated periplasmic glucans in Escherichia coli: the phosphoethanolamine transferase is encoded by opgE.
Osmoregulated periplasmic glucans (OPGs) are oligosaccharides found in the periplasm of many Gram-negative bacteria. Glucose is the sole constitutive sugar and this backbone may be substituted by various kinds of molecules depending on the species. In E. coli, OPG are substituted by phosphoglycerol and phosphoethanolamine derived from membrane phospholipids and by succinyl residues. In this study, we describe the isolation of the opgE gene encoding the phosphoethanolamine transferase by a screen previously used for the isolation of the opgB gene encoding the phosphoglycerol transferase. Both genes show structural and functional similarities without sequence similarity.
Unité de Glycobiologie Structurale et Fonctionnelle, UMR CNRS 8576, IFR 147, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq Cedex, France ; Université Lille Nord de France, Lille, France.
Sébastien
S
Bontemps-Gallo
Virginie
V
Cogez
Catherine
C
Robbe-Masselot
Kevin
K
Quintard
Jacqueline
J
Dondeyne
Edwige
E
Madec
Jean-Marie
JM
Lacroix
D004926:Escherichia coli / Q000235:genetics; D004982:Ethanolaminephosphotransferase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D004983:Ethanolamines / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005936:Glucans / Q000096:biosynthesis* / Q000235:genetics; D005947:Glucose / Q000235:genetics / Q000378:metabolism*; D019897:Periplasm / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D040681:Structural Homology, Protein; D017855:Transferases (Other Substituted Phosphate Groups) / Q000737:chemistry / Q000235:genetics*
D004983:Ethanolamines; D005936:Glucans; C005448:phosphorylethanolamine; D017855:Transferases (Other Substituted Phosphate Groups); D004982:Ethanolaminephosphotransferase; C037247:phosphatidylglycerol - membrane-oligosaccharide glycerophosphotransferase; D005947:Glucose