Facile and highly efficient approach for the fabrication of multifunctional silk nanofibers containing hydroxyapatite and silver nanoparticles.
In this study, a good combination consisting of electrospun silk fibroin nanofibers incorporated with high-purity hydroxyapatite (HAp) nanoparticles (NPs) and silver NPs is introduced as antimicrobial for tissue engineering applications. The variable pressure field emission scanning electron microscope results confirmed randomly placed nanofibers are produced with highly dispersed HAp and silver NPs in nanofibers after electrospinning. The X-ray diffraction results demonstrated crystalline features of each of the three components used for electrospinning. Moreover, the TEM-EDS analysis confirmed the presence and chemical nature of each component over individual silk nanofiber. The FT-IR analyses was used confirm the different vibration modes caused due to functional groups present in silk fibroin, Hap, and silver NPs. The obtained nanofibers were checked for antimicrobial activity by using two model organisms Escherichia coli and Staphylococcus aureus. Subsequently, the antimicrobial tests have indicated that prepared nanofibers do possess good bactericidal activity. The ability of N,N-dimethylformamide and silk fibroin used to reduce silver nitrate into silver metal was evaluated using MTT assay. The nanofibers were grown in presence of NIH 3T3 fibroblasts, which revealed toxic behavior to fibroblasts at higher concentrations of silver nitrate used in this study. Furthermore, cell attachment studies on nanofibers for 3 and 12 days of incubation time were minutely observed and correlated with the results of MTT assay. The reported results confirmed the high amounts of silver nitrate can lead to toxic effects on viability of fibroblasts and had bad effect in cell attachment.
Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, 200-702, South Korea; Department of Chemistry, University of Texas-Pan American, Edinburg, Texas, 78539.
Faheem A
FA
Sheikh
Hyung Woo
HW
Ju
Bo Mi
BM
Moon
Hyun Jung
HJ
Park
Jung-Ho
JH
Kim
Ok Joo
OJ
Lee
Chan Hum
CH
Park
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D017886:Durapatite / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D005612:Freeze Drying; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D008826:Microbial Sensitivity Tests; D041681:NIH 3T3 Cells; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D047011:Silk / Q000494:pharmacology*; D012834:Silver / Q000494:pharmacology*; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects; D014961:X-Ray Diffraction
D047011:Silk; D012834:Silver; D017886:Durapatite
antimicrobials; biocompatible; biomaterials; cell attachment; cell viability; cytotoxicity; nanofibers; silk