Change of Nrf2 expression in rat hippocampus in a model of chronic cerebral hypoperfusion.
Chronic cerebral hypoperfusion (CCH) is common in vascular dementia and Alzheimer's disease. CCH-related oxidative damage plays a significant role in the development of cognitive impairment. Nuclear factor-erythroid 2-related factor-2 (Nrf2) mediates activation of the antioxidant responsive element (ARE)-related gene expression, which is crucial to the endogenous antioxidative system. In this case, we used permanent bilateral occlusion of common carotid arteries (2VO) to mimic CCH. The expression of Nrf2 in different regions of the hippocampus as well as the ability of nuclear Nrf2 and ARE binding have been examined. A phenomenon has been observed that the DNA binding activities were down-regulated. Interestingly, the expression of Nrf2 rose significantly in most regions of rat hippocampus within three weeks after the 2VO surgery. The mismatch might attribute to Nrf2 dysfunction and compensatory synthesis. A conclusion can be drawn that Nrf2 dysfunction is an important factor as a cause of CCH-induced oxidative damage and Nrf2 can be treated as a promising target to alleviate oxidative damage, even cognitive impairment caused by CCH.
Department of Neurology, Zhongnan Hospital, Wuhan University , Wuhan , China.
Ying
Y
Yang
Junjian
J
Zhang
Hui
H
Liu
Lei
L
Zhang
D000818:Animals; D002561:Cerebrovascular Disorders / Q000378:metabolism* / Q000473:pathology; D002908:Chronic Disease; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000098:blood supply* / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051267:NF-E2-Related Factor 2 / Q000096:biosynthesis*; D018384:Oxidative Stress / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat
Nuclear factor-erythroid 2-related factor-2; chronic cerebral hypoperfusion; hippocampus; oxidative stress