EBP1, a novel host factor involved in primer binding site-dependent restriction of moloney murine leukemia virus in embryonic cells.
Mouse embryonic cells are unable to support the replication of Moloney murine leukemia virus (MLV). The integrated viral DNA is transcriptionally silenced, largely due to binding of host transcriptional repressors to the primer binding site (PBS) of the provirus. We have previously shown that a PBS DNA-binding repressor complex contains ZFP809 and TRIM28. Here, we identified ErbB3-binding protein 1 (EBP1) to be a novel component of the ZFP809-TRIM28 silencing complex and show that EBP1 depletion reduces PBS-mediated retroviral silencing.
Integrated Program in Cellular, Molecular and Biophysical Studies, Columbia University, New York, New York, USA.
Gary Z
GZ
Wang
Daniel
D
Wolf
Stephen P
SP
Goff
D000818:Animals; D001665:Binding Sites; D045744:Cell Line, Tumor; D017931:DNA Primers / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D020868:Gene Silencing; D007938:Leukemia / Q000196:embryology / Q000235:genetics / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D051379:Mice; D008979:Moloney murine leukemia virus / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D012376:Rodent Diseases / Q000196:embryology / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D000076183:Tripartite Motif-Containing Protein 28; D014779:Virus Replication
D017931:DNA Primers; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C095938:Pa2g4 protein, mouse; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; C541321:ZFP809 protein, mouse; C103190:Trim28 protein, mouse; D000076183:Tripartite Motif-Containing Protein 28