Plant regeneration from isolated microspore cultures of Chinese cabbage (Brassica campestris spp. pekinensis).
Isolated microspores of Chinese cabbage (Brassica campestris ssp. pekinensis) were incubated in modified NN medium containing 10% sucrose in darkness at 33°C for one day followed by culture at 25°C. After 14 days of culture, microspores developed into embryos ranging from globular to cotyledonary stage. Plants were regenerated after transfer of embryos to medium containing 3% sucrose and no plant growth regulators.
National Research Institute of Vegetables, Ornamental Plants and Tea (NIVOT) Ano, 514-23, Mie, Japan.
T
T
Sato
T
T
Nishio
M
M
Hirai