Elution kinetics, antimicrobial activity, and mechanical properties of 11 different antibiotic loaded acrylic bone cement.
Antibiotic-loaded acrylic bone cements (ALABC) spacers are routinely used in the treatment of prosthetic joint infections. The objectives of our study were to evaluate different ALABC for elution kinetics, thermal stability, and mechanical properties. A 10 or 20% mixture (w/w) beads of medium viscosity bone cement (DePuy, Inc) and vancomycin (VAN), gentamycin (GM), daptomycin (DAP), moxifloxacin (MOX), rifampicin (RIF), cefotaxime (CTX), cefepime (FEP), amoxicillin clavulanate (AmC), ampicillin (AMP), meropenem (MER), and ertapenem (ERT) were formed and placed into wells filled with phosphate-buffered saline. Antibiotic concentrations were determined using high-performance liquid chromatography. Antimicrobial activity was tested against Micrococcus luteus ATCC 9341 or Escherichia coli ATCC 25922. AmC, AMP, and FEP concentration rapidly decreased after day 2, being almost undetectable at day 4. Sustained and high elution rates were observed with VAN, GM, MOX, and RIF for the 30-day duration of the experiment. DAP, MER, ERT, and CTX elution rates constantly decreased from day 4. All antibiotics tested retained antimicrobial activity proving thermal stability. Mechanical properties of ALABC were maintained except when RIF was used.
Servicio de Enfermedades Infecciosa, Hospital Universitario San Cecilio, Granada, Spain.
Ruben
R
Gálvez-López
Service de Microbiología, Hospital Universitario San Cecilio, Granada, Spain.
Alejandro
A
Peña-Monje
Servicio de Cirugía Ortopédica y Traumatología, Hospital Universitario San Cecilio, Granada, Spain.
Ramón
R
Antelo-Lorenzo
Laboratorio Materiales de Construcción, ETS Ingenieros de Edificación, Universidad de Granada, Granada, Spain.
Juan
J
Guardia-Olmedo
Laboratorio de Espectometría de masas de alta resolución, Centro de Instrumentación Científica, Universidad de Granada, Granada, Spain.
Juan
J
Moliz
Servicio de Enfermedades Infecciosa, Hospital Universitario San Cecilio, Granada, Spain.
José
J
Hernández-Quero
Servicio de Enfermedades Infecciosa, Hospital Universitario San Cecilio, Granada, Spain. Electronic address: jordi@ugr.es.
Jorge
J
Parra-Ruiz
D000900:Anti-Bacterial Agents / Q000032:analysis / Q000493:pharmacokinetics* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D055595:Mechanical Phenomena; D008826:Microbial Sensitivity Tests; D008835:Micrococcaceae / Q000187:drug effects; D019904:Polymethyl Methacrylate / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors
D000900:Anti-Bacterial Agents; D019904:Polymethyl Methacrylate
Antibiotic loading; Compression strength; Elution kinetics