Study for the improvement of umbilical cord blood sampling using a new trial apparatus.
OBJECTIVE
The aim of this study was to evaluate the usefulness of the trial umbilical cord blood sampling bag for unrelated cord blood transplantation.


METHODS
Data were obtained from 100 vaginal deliveries. In 50 cases, umbilical cord blood (UCB) was taken with the traditional Kawasumi type UCB sampling bag. In another 50 cases, UCB were taken with trial UCB sampling bag offered by NIPRO Co. We compared the sampling volume between the two groups. Furthermore, 10 cases in each group were matched by sampling volume; we examined the quality of UCB on the number and concentration of nucleated cells, mononuclear cells, CD34+ cells and colony-forming unit granulocyte macrophage and the numbers tested positive for bacteria.


RESULTS
Whereas there were no significant differences in gestational weeks at sampling, the ratio of primipara women to multipara women, maternal age, and neonatal weight between the two groups, the sampling UCB volumes with the trial sampling bag were significantly higher than those with traditional sampling bags (P < 0.05). In addition, this phenomenon was more significant in the latter part of the study period (P < 0.05). On the other hand, there were no significant differences in the quality of UCB between the two groups.


CONCLUSIONS
Once clinicians have become accustomed to the trial UBC sampling bag, this method might be a useful method for collecting UCB for unrelated cord blood transplantation.
Department of Obstetrics and Gynecology, Tokyo Women's Medical University Yachiyo Medical Center, Chiba, Japan.
Naoki
N
Masaoka
Masako
M
Morooka
Yoshiyuki
Y
Nakajima
Hajime
H
Ogata
Hideki
H
Kodo
Shunichi
S
Kato
D000328:Adult; D001800:Blood Specimen Collection / Q000295:instrumentation*; D001810:Blood Volume; D036101:Cord Blood Stem Cell Transplantation; D005260:Female; D005312:Fetal Blood / Q000166:cytology / Q000382:microbiology; D006801:Humans
cord blood sampling; placenta; stem cell; unrelated cord blood transplantation