Biotransformation of (-)-codeinone to (-)-codeine byPapaver somniferum cells immobilized in reticulate polyurethane foam.
Papaver somniferum cells immobilized in reticulate-polyurethane foam biotransformed (-)-codeinone to (-)-codeine. A biotransformation ratio of 79% was found in immobilized cells whereas a ratio of 57% was found in suspended cells. Of the total amount of codeine formed only 12.2% was detected inside the cells, most of the product (87.3%) being released into the medium. When immobilized cells were cultivated in the absence of nitrate, only 40% of the cells remained alive and the biotransformation of codeinone was strongly reduced. When orthophosphate was omitted from the growth medium a bioconversion ratio of 86% was achieved.
Department of Botany, University of Edinburgh, The King's Buildings, Mayfield Road, EH9 3JH, Edinburgh, United Kingdom.
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Yeoman