Characterizations of cholinesterases in golden apple snail (Pomacea canaliculata).
Cholinesterases (ChEs) have been identified in vertebrates and invertebrates. Inhibition of ChE activity in invertebrates, such as bivalve molluscs, has been used to evaluate the exposure of organophosphates, carbamate pesticides, and heavy metals in the marine system. The golden apple snail (Pomacea canaliculata) is considered as one of the worst invasive alien species harmful to rice and other crops. The ChE(s) in this animal, which has been found recently, but poorly characterized thus far, could serve as biomarker(s) for environmental surveillance as well as a potential target for the pest control. In this study, the tissue distribution, substrate preference, sensitivity to ChE inhibitors, and molecular species of ChEs in P. canaliculata were investigated. It was found that the activities of both AChE and BChE were present in all test tissues. The intestine had the most abundant ChE activities. Both enzymes had fair activities in the head, kidney, and gills. The BChE activity was more sensitive to tetra-isopropylpyrophosphoramide (iso-OMPA) than the AChE. Only one BChE molecular species, 5.8S, was found in the intestine and head, whereas two AChE species, 5.8S and 11.6S, were found there. We propose that intestine ChEs of this snail may be potential biomarkers for manipulating pollutions.
Department of Biology, Hanshan Normal University, Chaozhou, 521041, Guangdong Province, China.
Xiang-Hui
XH
Zou
Heidi Qun-Hui
HQ
Xie
Guang-Cai
GC
Zha
Vicky Ping
VP
Chen
Yan-Jie
YJ
Sun
Yu-Zhong
YZ
Zheng
Karl Wah-Keung
KW
Tsim
Tina Ting-Xia
TT
Dong
Roy Chi-Yan
RC
Choi
Wilson Kin-Wai
WK
Luk
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002091:Butyrylcholinesterase / Q000378:metabolism*; D007422:Intestines / Q000201:enzymology; D009928:Organ Specificity; D012908:Snails / Q000201:enzymology*
D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase