Dishevelled-1 (Dvl-1) protein: a potential participant of oxidative stress induced by selenium deficiency.
Oxidative stress induced by selenium deficiency has been shown to be associated with cardiovascular diseases. Nevertheless, the mechanism associated with oxidative stress induced by selenium deficiency is poorly understood. In the present study, 36 weaning C57BL/6 mice were randomly divided into 4 groups as follows: control (n =9), 4-week selenium deficiency (n =9), 8-week selenium deficiency (n = 9), and 12-week selenium deficiency (n =9). The levels of myocardial glutathione peroxidase (GPx), superoxide dismutase (SOD), and malondialdehyde (MDA) were determined by Western blotting or commercial kits. Real-time PCR was performed to detect the mRNA expression of dishevelled-1 (Dvl-1) protein. Western blotting was conducted to evaluate the protein expression levels of Dvl-1 and β-catenin. Our results demonstrated that the levels of GPx and SOD were significantly reduced, along with an increase in MDA in selenium-deficient mice. Importantly, Dvl-1 and β-catenin were clearly upregulated under oxidative stress. Collectively, our findings indicate that Dvl-1 may be an underlying participant of oxidative stress induced by selenium deficiency.
Han-dong
HD
Zhao
Mei-na
MN
Sun
Meng-di
MD
Li
Feng-lan
FL
Li
Hui
H
Li
D048868:Adaptor Proteins, Signal Transducing / Q000502:physiology*; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D003677:Deficiency Diseases / Q000503:physiopathology*; D000072261:Dishevelled Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D010750:Phosphoproteins / Q000502:physiology*; D012643:Selenium / Q000172:deficiency*
D048868:Adaptor Proteins, Signal Transducing; D017931:DNA Primers; D000072261:Dishevelled Proteins; C000606579:Dvl1 protein, mouse; D010750:Phosphoproteins; D012643:Selenium