Effects of rhamnolipid biosurfactant JBR425 and synthetic surfactant surfyno1465 on the peroxidase-catalyzed oxidation of 2-naphthol.
The kinetics of the recombinant Coprinus cinereus peroxidase-catalyzed 2-naphthol oxidation was investigated in the presence of rhamnolipid biosurfactant JBR425 and synthetic surfactant Surfynol465 at pH 5.5 and 250C, with concentrations of (bio)surfactants both less than critical micelle concentrations (CMC) and larger than CMC. It was shown that monomers of JBR425 as well as monomers of Surfynol465 had an enhancing effect on the conversion of 2-naphthol in dose response manner and did not influence the initial rate of 2-naphthol oxidation. The results were accounted by a scheme, which contains a stadium of enzyme inhibition by oligomeric 2-naphthol oxidation products. The action of the biosurfactant's (or synthetic surfactant's) monomers was explained by avoidance of the enzyme active center clothing with oligomers. Similar results have demonstrated the potential of rhamnolipid biosurfactant JBR425 due to its biodegradability. When biosurfactants' concentrations are larger than CMC, (bio)surfactants have an opposite effect on the oxidation of 2-naphthol by peroxidase.
Department of Chemistry and Bioengineering, Faculty of Fundamental Sciences, Vilnius Gediminas Technical University, Vilnius 10223, Lithuania. ruta.ivanec-goranina@dok.vgtu.lt
Ivanec-Goranina
IG
Rūta
Kulys
K
Juozas
D002384:Catalysis; D003302:Coprinus / Q000201:enzymology; D006017:Glycolipids / Q000737:chemistry*; D009284:Naphthols / Q000737:chemistry*; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000737:chemistry*; D018508:Water Purification / Q000379:methods
D006017:Glycolipids; D009284:Naphthols; D011994:Recombinant Proteins; D013501:Surface-Active Agents; D014874:Water Pollutants, Chemical; C418382:rhamnolipid; D009195:Peroxidase; C028405:2-naphthol