Parental genome expression in synthetic wheats (Triticum turgidum sp. × T. tauschii sp.) revealed by two-dimensional electrophoresis of seedling proteins.
Two-dimensional gel electrophoresis was conducted on etiolated seedling proteins from two distinct amphiploids (ABD1, ABD2) and their parental lines (AB1, D1 and AB2, D2), AB1 and AB2 being used as female. On the amphiploid patterns were found all the parental spots except 8 D spots of which 3 are cytoplasmically encoded. One exceptional polypeptide observed in ABD1 was present neither in AB1 nor D1. The patterns fromt the amphiploids very closely resemble the co-electrophoresis done with 1/3 D protein extract and 2/3 AB protein extract. Thus it is very likely that for most gene products revealed the genomes act independently of each other.
Laboratoire de Génétique des Systèmes Végétaux, INRA-CNRS-UPS, La Ferme du Moulon, F-91190, Gif-sur-Yvette, France.
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Bahrman
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Thiellement