Myricitrin inhibits acrylamide-mediated cytotoxicity in human Caco-2 cells by preventing oxidative stress.
Oxidative stress was thought to be associated with acrylamide cytotoxicity, but the link between oxidative stress and acrylamide cytotoxicity in the gastrointestinal tract, the primary organ in contact with dietary acrylamide, is still unclear. This study was conducted to evaluate the antioxidant activity of natural dietary compound myricitrin and its protective role against acrylamide cytotoxicity. We found that myricitrin can effectively scavenge multiple free radicals (including DPPH free radical, hydroxyl radical, and ABTS free radical) in a concentration-dependent manner. Our results further indicated that the presence of myricitrin (2.5-10 μg/mL) was found to significantly inhibit acrylamide-induced cytotoxicity in human gastrointestinal Caco-2 cells. Moreover, acrylamide-induced cytotoxicity is closely related to oxidative stress in Caco-2 cells. Interestingly, myricitrin was able to suppress acrylamide toxicity by inhibiting ROS generation. Taken together, these results demonstrate that myricitrin had a profound antioxidant effect and can protect against acrylamide-mediated cytotoxicity.
Department of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang Key laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China.
Wei
W
Chen
Lina
L
Feng
Yang
Y
Shen
Hongming
H
Su
Ya
Y
Li
Jingjing
J
Zhuang
Lingxia
L
Zhang
Xiaodong
X
Zheng
D020106:Acrylamide / Q000633:toxicity; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D018938:Caco-2 Cells; D049109:Cell Proliferation / Q000187:drug effects*; D019587:Dietary Supplements / Q000633:toxicity; D005419:Flavonoids / Q000494:pharmacology*; D041981:Gastrointestinal Tract / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism
D000975:Antioxidants; D005419:Flavonoids; D017382:Reactive Oxygen Species; D020106:Acrylamide; C008577:myricitrin