Cell differentiation in protoplast cultures from embryogenic callus ofAbies alba L.
Protoplasts were isolated from embryogenic callus ofAbies alba L. which originated from immature seeds. The protoplasts were immobilized in alginate beads in order to follow the development of single protoplasts.Surrounding culture medium was modified from Kao and Michayluk (1975). After cell wall regeneration subsequent cell divisions lead to the formation of colonies showing an early differentiation of small meristematic cells and large vacuolated "suspensor-type" cells.
Botanisches Institut der Johann Wolfgang Goethe-Universität, Siesmayerstrasse 70, D-6000, Frankfurt am Main, Federal Republic of Germany.
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Kohlenbach