[Authenticate of Atractylodes macrocephala seed by amplification refractory mutation system].
OBJECTIVE
To design specific primers and authenticate Atractylodes macrocephala from Atractylodes lancea and A. chinensis.


METHODS
SNPs in the psbA-trnH sequences of Atractylodes were found by ClustulW program and Bioedit software. Primers for authentic A. macrocephala is designed according to the SNP site, and ITS sequence universal primers plus to the authentic primer to construct a multi-PCR reaction system, and then optimized the PCR reaction system.


RESULTS
172 bp band special for A. macrocephala were found using multi-PCR reaction.


CONCLUSIONS
The multi-PCR reaction system could be applied to identify A. macrocephala seed.
National Resource Center for Chinese Materia Medica, China Academy of Chinese Medicinal Sciences, Beijing 100700, China.
Liang
L
Cao
Chao
C
Jiang
Hua-Sheng
HS
Peng
Min
M
Chen
Yuan
Y
Yuan
D031174:Atractylodes / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D011786:Quality Control; D012639:Seeds / Q000235:genetics*
D017931:DNA Primers