Alpha-lipoic acid protects cardiomyocytes against hypoxia/reoxygenation injury by inhibiting autophagy.
Hypoxia/reoxygenation (H/R) is an important in vitro model for exploring the molecular mechanisms and functions of autophagy during myocardial ischemia/reperfusion (I/R). Alpha-lipoic acid (LA) plays an important role in the etiology of cardiovascular disease. Autophagy is widely implicated in myocardial I/R injury. We assessed the degree of autophagy by pretreatment with LA exposed to H/R in H9c2 cell based on the expression levels of Beclin-1, LC3II/LC3I, and green fluorescent protein-labeled LC3 fusion proteins. Autophagic vacuoles were confirmed in H9c2 cells exposed to H/R using transmission electron microscopy. Our findings indicated that pretreatment with LA inhibited the degree of autophagy in parallel to the enhanced cell survival and decreased total cell death in H9c2 cells exposed to H/R. We conclude that LA protects cardiomyocytes against H/R injury by inhibiting autophagy.
Department of Cardiology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Xueming
X
Cao
Aihua
A
Chen
Pingzhen
P
Yang
Xudong
X
Song
Yingfeng
Y
Liu
Zhiliang
Z
Li
Xianbao
X
Wang
Lizi
L
Wang
Yunpeng
Y
Li
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001343:Autophagy / Q000187:drug effects*; D000071186:Beclin-1; D002478:Cells, Cultured; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000648:ultrastructure; D020011:Protective Agents / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D008063:Thioctic Acid / Q000494:pharmacology*; D014617:Vacuoles / Q000378:metabolism / Q000648:ultrastructure
D051017:Apoptosis Regulatory Proteins; D000071186:Beclin-1; D049452:Green Fluorescent Proteins; D008869:Microtubule-Associated Proteins; D020011:Protective Agents; D011993:Recombinant Fusion Proteins; D008063:Thioctic Acid; C512829:Becn1 protein, rat; C526109:Anxa3 protein, rat
Alpha-lipoic acid; Autophagy; Cardiomyocytes; Hypoxia/reoxygenation