An endogenous promoter for conditional gene expression in Acremonium chrysogenum: the xylan and xylose inducible promoter xyl1(P.).
Acremonium chrysogenum is the natural producer of the beta-lactam antibiotic cephalosporin C and therefore of significant biotechnological importance. Here we identified and characterized the xylanase-encoding xyl1 gene and demonstrate that its promoter, xyl1(P), is suitable for conditional expression of heterologous genes in A. chrysogenum. This was shown by xylose and xylan-inducible xyl1(P)-driven expression of genes encoding green fluorescence protein and phleomycin resistance. Moreover, we demonstrate the potential of the xyl1(P) promoter for selection marker recycling. Taken together, these finding will help to overcome the limitation in genetic tools in this important filamentous fungus.
Christian Doppler Laboratory for Fungal Biotechnology, Division of Molecular Biology/Biocenter, Innrain 80-82, A-6020 Innsbruck, Austria.
Michael
M
Blatzer
Christian Doppler Laboratory for Fungal Biotechnology, Division of Molecular Biology/Biocenter, Innrain 80-82, A-6020 Innsbruck, Austria.
Fabio
F
Gsaller
Christian Doppler Laboratory for Fungal Biotechnology, Division of Molecular Biology/Biocenter, Innrain 80-82, A-6020 Innsbruck, Austria.
Beate
B
Abt
Christian Doppler Laboratory for Fungal Biotechnology, Division of Molecular Biology/Biocenter, Innrain 80-82, A-6020 Innsbruck, Austria; Sandoz GmbH, Biochemiestraße 10, 6250 Kundl, Austria.
Markus
M
Schrettl
Sandoz GmbH, Biochemiestraße 10, 6250 Kundl, Austria.
Thomas
T
Specht
Christian Doppler Laboratory for Fungal Biotechnology, Division of Molecular Biology/Biocenter, Innrain 80-82, A-6020 Innsbruck, Austria. Electronic address: hubertus.haas@i-med.ac.at.
Hubertus
H
Haas
D000164:Acremonium / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000235:genetics; D010692:Phleomycins / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014990:Xylans / Q000494:pharmacology*; D014994:Xylose / Q000494:pharmacology*; D014995:Xylosidases / Q000235:genetics*
D010692:Phleomycins; D014990:Xylans; D014994:Xylose; D014995:Xylosidases
Cephalosporin; Inducible promoter; Selection marker-recycling; Xylan/xylose-dependent gene expression; Xylanase