Multiple reconstruction of barley karyotype resulting in complete cytological marking of the chromosome complement.
A new reconstructed barley karyotype, PK88, which is a quadruple homozygote for three unequal translocations, 1-2, 3-4, 5-7, and one pericentric inversion in chromosome 6, was studied. As a result of these chromosome rearrangements, a complete cytological marking of the complement has been achieved. Due to the specific intra or interchromosomal transfer of particular bands, Giemsa staining of somatic chromosomes provided clear-cut indications about the localization of translocation and inversion breakpoints. It was established that the long arms of chromosomes 1, 2, 4, 5 and 7 and the short arm of chromosome 3 have been involved in interchanges 1-2, 3-4, and 5-7. The breakpoints of pericentric inversion proved to be located proximally to the short (satellite) arm and distally in the long arm of chromosome 6. PK-88 offers an essential gain in resolution power and extension of the areas of application in cytogenetics over other reconstructed karyotypes produced so far in barley.
Institute of Genetics, 1113, Sofia, Bulgaria.
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Gecheff