Reversible variations in the methylation pattern of carrot DNA during somatic embryogenesis.
Twenty five clones from a carrot genomic library were used as probes to detect variations in the 5-methyl-cytosine pattern during somatic embryogenesis. The majority of them evidenced an invariant pattern. With two clones however, differences were found between the adulttype DNA pattern and the embryonic one. The level of transcription of the relevant DNA fragments during embryogenesis was investigated by Northern blotting. One of the two probes did not show signals in any of the developmental stages whereas the other showed developmentally regulated expression. This fact suggests a novel strategy for cloning developmentally expressed functions.
Istituto di Mutagenesi e Differenziamento, CNR, Via Svezia 10, 2A, I-56124, Pisa, Italy.
R
R
Vergara
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F
Verde
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L
Pitto
F
F
Lo Schiavo
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M
Terzi