Mussel oligopeptides protect human fibroblasts from hydrogen peroxide (H2O2)-induced premature senescence.
Mussel bioactive peptides have been viewed as mediators to maximize the high quality of life. In this study, the anti-aging activities of mussel oligopeptides were evaluated using H2O2-induced prematurely senescent MRC-5 fibroblasts. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry displayed that exposure to H2O2 led to the loss of cell viability and cell cycle arrest. In addition, H2O2 caused the elevation of senescence-associated-β-galactosidase (SA-β-gal) activity and formation of senescence-associated heterochromatin foci (SAHF). It was found that pretreatment with mussel oligopeptides could significantly attenuate these properties associated with cellular senescence. Mussel oligopeptides also led to the increase of glutathione (GSH) level and mitochondrial transmembrane potential (Δψm) recovery. In addition, mussel oligopeptides resulted in an improvement in transcriptional activity of peroxiredoxin 1 (Prx1), nicotinamide phosphoribosyltransferase (NAMPT) and sirtuin 1 (SIRT1). This study revealed that mussel oligopeptides could protect against cellular senescence induced by H2O2, and the effects were closely associated with redox cycle modulating and potentiating the SIRT1 pathway. These findings provide new insights into the beneficial role of mussel bioactive peptides on retarding senescence process.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Yue
Y
Zhou
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: ydong@ujs.edu.cn.
Ying
Y
Dong
Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
Qing-Gang
QG
Xu
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Shu-Yun
SY
Zhu
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Shi-Lei
SL
Tian
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Jing-jing
JJ
Huo
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Ting-Ting
TT
Hao
School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
Bei-Wei
BW
Zhu
D000375:Aging; D000818:Animals; D049872:Bivalvia; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology*; D016922:Cellular Senescence / Q000187:drug effects / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology*; D009842:Oligopeptides / Q000378:metabolism* / Q000494:pharmacology; D016877:Oxidants / Q000378:metabolism / Q000494:pharmacology*; D054464:Peroxiredoxins; D011487:Protein Conformation; D056564:Sirtuin 1 / Q000378:metabolism; D013778:Tetrazolium Salts; D013844:Thiazoles; D001616:beta-Galactosidase / Q000378:metabolism
D009842:Oligopeptides; D016877:Oxidants; D013778:Tetrazolium Salts; D013844:Thiazoles; D006861:Hydrogen Peroxide; D054464:Peroxiredoxins; D001616:beta-Galactosidase; C447939:SIRT1 protein, human; D056564:Sirtuin 1; C022616:thiazolyl blue
3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; CK; DMSO; GSH; MTT; MW; Mussel oligopeptides; NAMPT; PSC; Premature senescence; Prx1; Redox cycle controlling; SA-β-gal; SAHF; SIRT1; SIRT1 pathway; control experiment; dimethylsulfoxide; glutathione; mitochondrial transmembrane potential; molecular weight; nicotinamide phosphoribosyltransferase; peroxiredoxin 1; premature senescence control; senescence-associated beta-galactosidase; senescence-associated heterochromatin foci; sirtuin 1; Δψm