Detection of CD59 using flow cytometry.
CD59 is the single regulator of the terminal complement pathway. It has been implicated in disease such as Paroxysmal nocturnal hemoglobinuria (PMH) and cancer. Expression of CD59 protects normal and malignant cells from the cytotoxic potential of the complement system. Here we describe a method, which allows for studying its expression on the surface of cells.
Division of Transplantation Immunology and Mucosal Biology, MRC Centre for Transplantation, King's College London, Guy's Hospital, London, UK.
Martin
M
Kolev
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D018957:CD59 Antigens / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line; D005347:Fibroblasts; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D016130:Immunophenotyping; D051379:Mice
D000911:Antibodies, Monoclonal; D018957:CD59 Antigens