Isolation of chromosomal and plasmid DNA from Staphylococcus epidermidis.
The following describes noncommercial methods for the purification of genomic and plasmid DNA from S. epidermidis. These include both large-scale, high molecular weight and quick, small-scale chromosomal DNA extractions, and also a standard alkaline lysis method of plasmid preparation.
Department of Pathology and Microbiology, Center for Staphylococcal Research, University of Nebraska Medical Center, Omaha, NE, USA.
Jill K
JK
Lindgren
D002876:Chromosomes, Bacterial / Q000235:genetics; D004269:DNA, Bacterial / Q000302:isolation & purification*; D010957:Plasmids / Q000302:isolation & purification*; D013212:Staphylococcus epidermidis / Q000235:genetics*
D004269:DNA, Bacterial