T cell-depleted splenocytes from mice pre-immunized with neuroantigen in incomplete Freund's adjuvant involved in protection from experimental autoimmune encephalomyelitis.
Mice immunized with neuroantigens in incomplete Freund's adjuvant (IFA) are resistant to subsequent induction of experimental autoimmune encephalomyelitis (EAE). The mechanisms involved in this protection are complex. Studies on relevant CD4(+) or CD8(+) T cells, including effective and regulatory T cells, have been performed by others. In this work, the effects of CD4(-)-, CD8(-)- splenocytes on protection from EAE in C57BL/6 mice which were immunized with myelin oligodendrocyte glycoprotein 35-55 (MOG)35-55 in IFA were evaluated. We observed that MOG-reactive CD4(+) T cells failed to be activated and proliferate when CD4(-)-, CD8(-)- splenocytes from MOG/IFA-immunized mice were regarded as antigen-presenting cells (APC). It was shown that these APC expressed lower levels of major histocompatibility complex class II (MHC-II), CD80, and CD86 than naïve cells. In addition, CD4(-)-, CD8(-)- splenocytes from MOG/IFA-immunized mice showed significantly higher levels of IL-10 mRNA expression. When the immunized-mice were induced to develop EAE, these cells secreted significantly higher levels of IL-10 and produced lower levels of IL-6, leading to decreased secretion of IL-17 and IFN-γ from MOG-specific CD4(+) T cells. The transfer of CD4(-)-, CD8(-)- splenocytes from MOG/IFA-immunized mice was able to ameliorate the subsequent induction of EAE in recipient mice. Thus, MOG/IFA immunization can modulate CD4(-)-, CD8(-)- splenocytes by reducing the expression of antigen-presenting molecules and altering the levels of secreted cytokines. Our study reveals an additional mechanism involved in the protective effects of MOG/IFA pre-immunization in an EAE model.
Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai, China; Department of Clinical Laboratory, Shanghai Cancer Center, Fudan University, Shanghai, China.
Hui
H
Zheng
Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Han
H
Zhang
Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Feng
F
Liu
Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Yuanyuan
Y
Qi
Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai, China; Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, USA. Electronic address: hong_jiang24@163.com.
Hong
H
Jiang
D019264:Adoptive Transfer; D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000276:immunology; D000941:Antigens / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000517:prevention & control*; D005620:Freund's Adjuvant / Q000276:immunology*; D007114:Immunization; D008055:Lipids / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D008212:Lymphocyte Depletion; D008297:Male; D051379:Mice; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D013154:Spleen / Q000166:cytology* / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism
D000941:Antigens; D000954:Antigens, Surface; D016207:Cytokines; D008055:Lipids; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; C114843:incomplete Freund's adjuvant; C445081:myelin oligodendrocyte glycoprotein (35-55); D005620:Freund's Adjuvant
APC; Antigen-presenting cell; CFA; CNS; EAE; Experimental autoimmune encephalomyelitis; IFA; Incomplete Freund's adjuvant; LPS; MFI; MHC-II; MOG; MS; PBS; SEM; antigen-presenting cells; central nervous system; complete Freunds adjuvant; counts per minute; cpm; experimental autoimmune encephalomyelitis; incomplete Freund's adjuvant; lipopolysaccharide; major histocompatibility complex class II; mean fluorescence intensity; multiple sclerosis; myelin oligodendrocyte glycoprotein; phosphate buffered saline; standard error of mean