Transcription analysis of two Eomesodermin genes in lymphocyte subsets of two teleost species.
Eomesodermin (Eomes), a T-box transcription factor, is a key molecule associated with function and differentiation of CD8(+) T cells and NK cells. Previously, two teleost Eomes genes (Eomes-a and -b), which are located on different chromosomes, were identified and shown to be expressed in zebrafish lymphocytes. For the present study, we identified these genes in rainbow trout and ginbuna crucian carp. Deduced Eomes-a and -b amino acid sequences in both fish species contain a highly conserved T-box DNA binding domain. In RT-PCR, both Eomes transcripts were readily detectable in a variety of tissues in rainbow trout and ginbuna. The high expression of Eomes-a and -b in brain and ovary suggests involvement in neurogenesis and oogenesis, respectively, while their expression in lymphoid tissues presumably is associated with immune functions. Investigation of separated lymphocyte populations from pronephros indicated that both Eomes-a and -b transcripts were few or absent in IgM(+) lymphocytes, while relatively abundant in IgM(-)/CD8α(+) and IgM(-)/CD8α(-) populations. Moreover, we sorted trout CD8α(+) lymphocytes from mucosal and non-mucosal lymphoid tissues and compared the expression profiles of Eomes-a and -b with those of other T cell-related transcription factor genes (GATA-3, T-bet and Runx3), a Th1 cytokine gene (IFN-γ) and a Th2 cytokine gene (IL-4/13A). Interestingly, the tissue distribution of Eomes-a/b, T-bet, and Runx3 versus IFN-γ transcripts did not reveal simple correlations, suggesting tissue-specific properties of CD8α(+) lymphocytes and/or multiple modes that drive IFN-γ expressions.
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute for Infectiology, Insel Riems, Germany; Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa, Japan.
Fumio
F
Takizawa
Faculty of Fisheries, Kagoshima University, Shimoarata, Kagoshima 890-0056, Japan.
Kyosuke
K
Araki
Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa, Japan.
Maki
M
Ohtani
Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa, Japan.
Hideaki
H
Toda
Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa, Japan.
Yasutaka
Y
Saito
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute for Infectiology, Insel Riems, Germany.
Veronica Soto
VS
Lampe
Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi, Japan.
Johannes Martinus
JM
Dijkstra
Aquatic Animal Health Division, National Research Institute of Aquaculture, Fisheries Research Agency, 422-1 Minami-Ise, Mie 516-0193, Japan.
Mitsuru
M
Ototake
Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa, Japan.
Tadaaki
T
Moritomo
Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa, Japan.
Teruyuki
T
Nakanishi
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute for Infectiology, Insel Riems, Germany. Electronic address: uwe.fischer@fli.bund.de.
Uwe
U
Fischer
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002347:Carps / Q000235:genetics / Q000276:immunology*; D020869:Gene Expression Profiling / Q000662:veterinary; D008221:Lymphoid Tissue / Q000276:immunology; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics / Q000276:immunology*; D010802:Phylogeny; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020825:T-Box Domain Proteins / Q000235:genetics / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D020825:T-Box Domain Proteins; D012313:RNA
CD8α+ T cells; Eomes; Ginbuna crucian carp; IFN-γ; Rainbow trout