MiRNAs which target CD3 subunits could be potential biomarkers for cancers.
BACKGROUND
T-cells play an important role in the immune response and are activated in response to the presentation of antigens bound to major histocompatibility complex (MHC) molecules participating with the T-cell receptor (TCR). T-cell receptor complexes also contain four CD3 (cluster of differentiation 3) subunits. The TCR-CD3 complex is vital for T-cell development and plays an important role in intervening cell recognition events. Since microRNAs (miRNAs) are highly stable in blood serum, some of which may target CD3 molecules, they could serve as good biomarkers for early cancer detection. The aim of this study was to see whether there is a relationship between cancers and the amount of miRNAs -targeted CD3 molecules.


METHODS
Bioinformatics tools were used in order to predict the miRNA targets for these genes. Subsequently, these highly conserved miRNAs were evaluated to see if they are implicated in various kinds of cancers. Consequently, human disease databases were used. According to the latest research, this study attempted to investigate the possible down- or upregulation of miRNAs cancer patients.


RESULTS
We identified miRNAs which target genes producing CD3 subunit molecules. The most conserved miRNAs were identified for the CD3G gene, while CD247 and CD3EAP genes had the least number and there were no conserved miRNA associated with the CD3D gene. Some of these miRNAs were found to be responsible for different cancers, following a certain pattern.


CONCLUSIONS
It is highly likely that miRNAs affect the CD3 molecules, impairing the immune system, recognizing and destroying cancer tumor; hence, they can be used as suitable biomarkers in distinguishing cancer in the very early stages of its development.
Department of Bioinformatics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Fariborz
F
Asghari Alashti
Zarrin
Z
Minuchehr
D014408:Biomarkers, Tumor / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D017252:CD3 Complex / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D009369:Neoplasms / Q000235:genetics / Q000276:immunology / Q000401:mortality*; D012334:RNA, Neoplasm / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
D014408:Biomarkers, Tumor; D017252:CD3 Complex; D035683:MicroRNAs; D012334:RNA, Neoplasm