Extent of genetic variability of endosperm esterases in Triticum aestivum L. 2n=6x=42.
Genetic variability of endosperm esterase has been studied in 42 cultivars of Triticum aestivum L. 2n=6x=42. Different techniques, including sequential electrophoresis and electrofocusing, have been used with various substrates and esterase inhibitors. The electrophoretic patterns in each cultivar are described. Chromosomal location using the nullitetrasomic and ditelosomic lines of Chinese Spring was carried out in order to relate and/or locate the esterase genes to specific chromosomes. Most of the esterase isozymes located were in the long arm of the chromosomes of the homoeology group 3; but we have found six located in the short arms, five of them in the chromosome 3AS and one in the 3DS. This location increases the number of esterase genes described, because no esterase genes had been described so far in short arms of chromosomes of the homoeology group 3. The genetic control is discussed and, according to our results, between 12 and 15 loci, organized in five "compound loci", control the endosperm esterases in wheat. Also one "modifier" gene modifying the mobility of two esterase bands and present in all the cultivars studied is postulated.
Departamento de Genetica, Facultad de Biologia, Universidad de Leon, E-24071, Leon, Spain.
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