Glucose inhibits root meristem growth via ABA INSENSITIVE 5, which represses PIN1 accumulation and auxin activity in Arabidopsis.
Glucose functions as a hormone-like signalling molecule that modulates plant growth and development in Arabidopsis thaliana. However, the role of glucose in root elongation remains elusive. Our study demonstrates that high concentrations of glucose reduce the size of the root meristem zone by repressing PIN1 accumulation and thereby reducing auxin levels. In addition, we verified the involvement of ABA INSENSITIVE 5 (ABI5) in this process by showing that abi5-1 is less sensitive to glucose than the wild type, whereas glucose induces ABI5 expression and the inducible overexpression of ABI5 reduces the size of the root meristem zone. Furthermore, the inducible overexpression of ABI5 in PIN1::PIN1-GFP plants reduces the level of PIN1-GFP, but glucose reduces the level of PIN1-GFP to a lesser extent in abi5-1 PIN1::PIN1-GFP plants than in the PIN1::PIN1-GFP control, suggesting that ABI5 is involved in glucose-regulated PIN1 accumulation. Taken together, our data suggest that ABI5 functions in the glucose-mediated inhibition of the root meristem zone by repressing PIN1 accumulation, thus leading to reduced auxin levels in roots.
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
Ting-Ting
TT
Yuan
Heng-Hao
HH
Xu
Kun-Xiao
KX
Zhang
Ting-Ting
TT
Guo
Ying-Tang
YT
Lu
D017360:Arabidopsis / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D049452:Green Fluorescent Proteins / Q000032:analysis; D007210:Indoleacetic Acids / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000032:analysis / Q000378:metabolism*; D018519:Meristem / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000032:analysis
C409743:ABI5 protein, Arabidopsis; D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; C116363:PIN1 protein, Arabidopsis; D010937:Plant Growth Regulators; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D005947:Glucose
ABA; ABI5; PIN1; auxin; glucose; root meristem zone