Unusual ECD fragmentation attributed to gas-phase helix formation in a conformationally dynamic peptide.
The helix-forming character of a model decapeptide, L4PL4K, is determined in the absence of solvent using ion mobility mass spectrometry, electron capture dissociation and molecular mechanics simulations. Unusual ECD fragmentation patterns dominated by b ions are attributed to helix formation upon electron capture and as a signature of conformational dynamics.
School of Chemistry, University of Edinburgh, Joseph Black Building, King's Buildings, West Mains Road, Edinburgh, UKEH3 9JJ. perdita.barran@ed.ac.uk.
Jason M D
JM
Kalapothakis
Yana
Y
Berezovskaya
Cleidiane G
CG
Zampronio
Peter A
PA
Faull
Perdita E
PE
Barran
Helen J
HJ
Cooper
D000595:Amino Acid Sequence; D005740:Gases / Q000737:chemistry; D013058:Mass Spectrometry; D056004:Molecular Dynamics Simulation; D009842:Oligopeptides / Q000737:chemistry*; D017433:Protein Structure, Secondary
D005740:Gases; D009842:Oligopeptides