Temperature-dependent behavior of immobilized alkaline phosphatase. I. Role of working conditions and pH during coupling.
Alkaline phosphatase from hog intestine was immobilized to controlled-pore glass under various conditions. The specific activity of the enzyme was not diminished by immobilization. The influence of temperature and pH on the behavior and the stability of the immobilized enzyme preparations is discussed and compared to that of the native enzyme.
Institut für Allgemeine Biochemie der UniversitÄt Wien and Ludwig Boltzmann Forschungsstelle, WÄhringerstraΒe 38, 1090, Wien, Austria.
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