Genome-wide analysis and expression profiling of the Solanum tuberosum aquaporins.
Aquaporins belongs to the major intrinsic proteins involved in the transcellular membrane transport of water and other small solutes. A comprehensive genome-wide search for the homologues of Solanum tuberosum major intrinsic protein (MIP) revealed 41 full-length potato aquaporin genes. All potato aquaporins are grouped into five subfamilies; plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), NOD26-like intrinsic proteins (NIPs), small basic intrinsic proteins (SIPs) and x-intrinsic proteins (XIPs). Functional predictions based on the aromatic/arginine (ar/R) selectivity filters and Froger's positions showed a remarkable difference in substrate transport specificity among subfamilies. The expression pattern of potato aquaporins, examined by qPCR analysis, showed distinct expression profiles in various organs and tuber developmental stages. Furthermore, qPCR analysis of potato plantlets, subjected to various abiotic stresses revealed the marked effect of stresses on expression levels of aquaporins. Taken together, the expression profiles of aquaporins imply that aquaporins play important roles in plant growth and development, in addition to maintaining water homeostasis in response to environmental stresses.
Department of Molecular Biotechnology, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, Seoul, Republic of Korea.
Jelli
J
Venkatesh
Jae-Woong
JW
Yu
Se Won
SW
Park
D000595:Amino Acid Sequence; D020346:Aquaporins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D017343:Genes, Plant; D018745:Genome, Plant; D008969:Molecular Sequence Data; D063245:Plant Development; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018514:Plant Structures / Q000378:metabolism*; D035281:Plant Tubers; D011198:Solanum tuberosum / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013312:Stress, Physiological
D020346:Aquaporins; D010940:Plant Proteins; C110065:major intrinsic protein, plant
AQPs; Abiotic stress; Aquaporin; GLPs; Homologues; MIPs; Major intrinsic proteins; NIPs; NJ; NOD26-like intrinsic proteins; PGSC; PIPs; Phylogenetics; SIPs; Solanum tuberosum; TIPs; XIPs; aquaporins; major intrinsic proteins; neighbour-joining; plasma membrane intrinsic proteins; potato genome sequencing consortium; quaglyceroporins; small basic intrinsic proteins; tonoplast intrinsic proteins; x-intrinsic proteins