Common Prairie feeds with different soluble and insoluble fractions used for CPM diet formulation in dairy cattle: impact of carbohydrate-protein matrix structure on protein and other primary nutrient digestion.
An experiment was conducted to investigate the relationship of carbohydrates molecular spectral characteristics to rumen degradability of primary nutrients in Prairie feeds in dairy cattle. In total, 12 different types of feeds were selected, each type of feed was from three different source with total 37 samples. Six types of them were energy-sourced feeds and the others were protein-sourced feeds. The carbohydrates molecular spectral intensity of various functional groups were collected using Fourier transform infrared attenuated total reflectance (ATR-FT/IR) spectroscopy. In the in situ study, the results showed that the rumen digestibility and digestible fractions of primary nutrients (DM, OM, NCP, and CP) were significantly different (P<0.05) among the feeds. The spectral bands features were significantly different (P<0.05) among the feeds. Spectral intensities of A_Cell, H_1415 and H_1370 were weakly positively correlated with in situ rumen digestibility and digestible fractions of DM, OM and NCP. Spectral intensities of H_1150, H_1015, A_1, and A_3 were weakly negatively associated with in situ rumen degradation of CP. Spectral intensities of A_1240 and H_1240, mainly associated with cellulosic compounds, were correlated with rumen CP degradation. The multiple regression analysis demonstrated that the spectral intensities of A_3 and H_1415 played the most important role and could be used as a potential tool to predict rumen protein degradation of feeds in dairy cattle. In conclusion, this study showed that the carbohydrates as a whole have an effect on protein rumen degradation, rather than cellulose alone, indicating carbohydrate-protein matrix structure impact protein utilization in dairy cattle. The non-invasive molecular spectral technique (ATR-FT/IR) could be used as a rapid potential tool to predict rumen protein degradation of feedstuffs by using molecular spectral bands intensities in carbohydrate fingerprint region.
Department of Animal and Poultry Science, College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada; Animal Nutrition Institute, Sichuan Agriculture University, Sichuan 625014, China.
Quanhui
Q
Peng
Animal Nutrition Institute, Sichuan Agriculture University, Sichuan 625014, China.
Zhisheng
Z
Wang
Department of Animal Science, Tianjin Agricultural University, Tianjin 300384, China.
Xuewei
X
Zhang
Department of Animal and Poultry Science, College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada; Department of Animal Science, Tianjin Agricultural University, Tianjin 300384, China. Electronic address: Peiqiang.yu@usask.ca.
Peiqiang
P
Yu
D000821:Animal Feed; D000818:Animals; D002241:Carbohydrates / Q000737:chemistry*; D002417:Cattle; D016000:Cluster Analysis; D003612:Dairying; D004032:Diet; D004044:Dietary Proteins / Q000737:chemistry*; D004063:Digestion / Q000502:physiology*; D017753:Ecosystem; D015999:Multivariate Analysis; D012044:Regression Analysis; D012417:Rumen / Q000378:metabolism; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared
D002241:Carbohydrates; D004044:Dietary Proteins
Carbohydrate–protein matrix structure; Molecular spectral bands; Rumen degradability