Detection of gamma-irradiation effect on DNA and protein using magnetic sensor and cyclic voltammetry.
In this study, a magnetic sensor utilizing Planar Hall Resistance (PHR) and cyclic Voltammetry (CV) for detecting the radiation effect was fabricated. Specifically, we applied in parallel a PHR sensor and CV device to monitor the irradiation effect on DNA and protein respectively. Through parallel measurements, we demonstrated that the PHR sensor and CV are sensitive enough to measure irradiation effect. The PHR voltage decreased by magnetic nanobead labeled DNA was slightly recovered after gamma ray irradiation. The behavior of cdk inhibitor protein p21 having a sandwich structure of Au/protein G/Ab/Ag/Ab was checked by monitoring the cyclic Voltammetry signal in analyzing the gamma ray irradiation effect.
Korea Atomic Energy Research Institute, Daejeon 305-600, Daejeon, Korea.
Duck-Gun
DG
Park
Hoon
H
Song
M B
MB
Kishore
G
G
Vértesy
Duk-Hyun
DH
Lee
D015374:Biosensing Techniques / Q000295:instrumentation*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000737:chemistry* / Q000528:radiation effects*; D004247:DNA / Q000235:genetics* / Q000528:radiation effects*; D004249:DNA Damage / Q000235:genetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005720:Gamma Rays; D018189:Immunomagnetic Separation / Q000295:instrumentation*; D011829:Radiation Dosage; D014159:Transducers
C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D004247:DNA