Electrophysiological and behavioral responses of the whitestriped longhorned beetle, Batocera lineolata, to the diurnal rhythm of host plant volatiles of holly, Viburnum awabuki.
The adsorption method of Tenax-TA absorbent with GC-MS was used to analyze diurnal rhythms of volatiles from undamaged holly plants, Viburnum awabuki Kock (Dipsacales: Adoxaceae) holly infested by the white-striped longhorned beetle, Batocera lineolata Chevrolat (Coleoptera: Cerambycidae). Electroantennography and a Y-tube olfactometer were used to compare and analyze electroantennogram and behavioral responses of unmated male and female adults to the volatiles from V. awabuki (both undamaged and infested plants). The results of the GC-MS analysis showed that phytosterol and alkane are major volatiles for V. awabuki. The relative content of V. awabuki volatiles changed during the day. Electroantennogram and behavioral responses of unmated male and female adults to the volatiles from both undamaged and infested plants of V. awabuki were stronger between 08:00 and 10:00 and 16:00 and 18:00, which is consistent with early morning and evening feeding behaviors of adults in the field.
1 Key Laboratory of Ecological Forestry Engineering of Sichuan Province, College of Forestry, Sichuan Agricultural University, Ya'an, Sichuan 625014, China.
Hua
H
Yang
Wei
W
Yang
Chun-Ping
CP
Yang
Tian-Hui
TH
Zhu
Qiong
Q
Huang
Shan
S
Han
Jiu-Jin
JJ
Xiao
D000818:Animals; D057506:Arthropod Antennae / Q000502:physiology; D002633:Chemotaxis; D002681:China; D002940:Circadian Rhythm; D001517:Coleoptera / Q000502:physiology*; D055724:Electrophysiological Phenomena; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D010572:Pest Control, Biological / Q000379:methods; D010675:Pheromones / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D027925:Viburnum / Q000737:chemistry* / Q000502:physiology; D055549:Volatile Organic Compounds / Q000378:metabolism*
D010675:Pheromones; D011108:Polymers; D055549:Volatile Organic Compounds; C010411:insect attractants; C010514:tenax