Impact of Desulfovibrio alaskensis biofilms on corrosion behaviour of carbon steel in marine environment.
Sulfate reducing prokaryotes are associated with the steel deterioration. They build heterogeneous biofilms, capable of accelerating corrosion processes. In this study metabolic activity and the biofilm development of Desulfovibrio alaskensis were correlated to electrochemical response of carbon steel surface. In the exponential growth phase sulfide concentration reached its maximum of about 10mM. This phenomenon was responsible for the parallel increase in the corrosion potential (Ecorr) up to -720mV (vs. SCE). Subsequently, during the intensive biofilm formation and development another Ecorr peak (-710mV vs. SCE) occurred. Decrease in Ecorr was registered during the biofilm maturation and kept stable, being 20mV lower than in the control. While carbon steel was protected from the microbial attachment and exposed to metabolic products, only one potential maximum (-730mV vs. SCE) was recorded. Here Ecorr variations coincided with sulfide concentration changes and kept at 120mV lower vs. the control. Weight loss examinations revealed corrosion rates, which did not exceed 0.05mm/y. Confocal microscopy suggested the importance of extracellular proteins in the biofilm formation. Above 150 proteins were detected in the EPS matrix. Surface effects of biofilm and metabolic products were visualised, revealing the role of attached microorganisms in the localised corrosion.
Biofilm Centre, Aquatische Biotechnologie, Fakultät für Chemie, Universität Duisburg-Essen, Essen, Germany.
Agata J
AJ
Wikieł
Biofilm Centre, Aquatische Biotechnologie, Fakultät für Chemie, Universität Duisburg-Essen, Essen, Germany.
Iaryna
I
Datsenko
Biofilm Centre, Aquatische Biotechnologie, Fakultät für Chemie, Universität Duisburg-Essen, Essen, Germany.
Mario
M
Vera
Biofilm Centre, Aquatische Biotechnologie, Fakultät für Chemie, Universität Duisburg-Essen, Essen, Germany. Electronic address: wolfgang.sand@uni-due.de.
Wolfgang
W
Sand
D018441:Biofilms / Q000254:growth & development*; D002244:Carbon / Q000737:chemistry*; D003343:Corrosion; D003901:Desulfovibrio / Q000502:physiology*; D055664:Electrochemical Techniques; D008420:Manufactured Materials / Q000032:analysis / Q000382:microbiology*; D013232:Steel / Q000737:chemistry*; D013499:Surface Properties
D013232:Steel; D002244:Carbon
Biofilms; Carbon steel; EPS; MIC; SRP