Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes.
The invasion of host cells by the intracellular protozoan Trypanosoma cruzi requires interactions with host cell molecules, and the replication of the parasite requires escape from a parasitophorous vacuole into the host cell cytosol. Galectin-3, a member of β-galactosidase-binding lectin family, has numerous extracellular and intracellular functions. In this study, we investigated the role of galectin-3 during the invasion and intracellular trafficking of T. cruzi extracellular amastigotes (EAs). Endogenous galectin-3 from mouse peritoneal macrophages accumulated around the pathogen during cell invasion by EAs. In addition, galectin-3 accumulated around parasites after their escape from the parasitophorous vacuole. Thus, galectin-3 behaved as a novel marker of phagolysosome lysis during the infection of host cells by T. cruzi.
Laboratório de Tripanosomatídeos, Disciplina de Imunologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas - Bloco 6T sala 07, Campus Umuarama, 38400-902 Uberlândia, Minas Gerais, Brasil.
Fabrício Castro
FC
Machado
Lilian
L
Cruz
Aline Alves
AA
da Silva
Mário Costa
MC
Cruz
Renato Arruda
RA
Mortara
Maria Cristina
MC
Roque-Barreira
Claudio Vieira
CV
da Silva
D000818:Animals; D001692:Biological Transport; D002478:Cells, Cultured; D003593:Cytoplasm / Q000469:parasitology; D053595:Embryonic Stem Cells / Q000469:parasitology; D004705:Endocytosis; D037502:Galectin 3 / Q000378:metabolism*; D006801:Humans; D017737:Macrophages, Peritoneal / Q000469:parasitology; D051379:Mice; D008810:Mice, Inbred C57BL; D011485:Protein Binding; D014349:Trypanosoma cruzi / Q000472:pathogenicity* / Q000502:physiology*
D037502:Galectin 3
Trypanosoma cruzi; galectin-3; intracellular traffic; phagolysosome lysis