Characteristics of some fermentative bacteria from a thermophilic methane-producing fermenter.
Anaerobic bacteria from a 55‡C methane-producing beef waste fermenter were enumerated, isolated, and characterized. Direct microscopic bacterial counts were 5.2-6.8×10(10) per g fermenter effluent. Using a nonselective roll-tube medium which contained 40% fermenter effluent, 8.5-14.1% of the microscopic count was culturable. Deletion of fermenter effluent significantly reduced the viable count. Sixty-four randomly picked strains were characterized. All were pleomorphic, gram-negative, anaerobic rods, many of which were difficult to grow in liquid media. The strains were divided into 5 major groups based on glucose fermentation, hydrogen sulfide production, starch hydrolysis, fermentation products, and morphology. Glucose was fermented by 75% of the isolates, 76% utilized starch, 25% produced hydrogen sulfide, 76% produced hydrogen, 37% produced indole, 21% hydrolyzed gelatin, and 13% were sporeformers. Ethanol, lactate, formate, acetate, and hydrogen were common fermentation products. Twenty-four representative strains had 1-12 flagella. Growth was observed between 35 and 73‡C. These studies indicate that species diversity among the isolated organisms was low.
Clay Center, USDA, ARS, Roman L. Hruska U.S. Meat Animal Research Center, 68933, Nebraska, USA.
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