Assessment of antioxidant and cytotoxicity activities of saponin and crude extracts of Chlorophytum borivilianum.
The present paper focused on antioxidant and cytotoxicity assessment of crude and total saponin fraction of Chlorophytum borivilianum as an important medicinal plant. In this study, three different antioxidant activities (2,2-diphenyl-1-picrylhydrazyl radical scavenging (DPPH), ferrous ion chelating (FIC), and β -carotene bleaching (BCB) activity) of crude extract and total saponin fraction of C. borivilianum tubers were performed. Crude extract was found to possess higher free radical scavenging activity (ascorbic acid equivalents 2578 ± 111 mg AA/100 g) and bleaching activity (IC50 = 0.7 mg mL(-1)), while total saponin fraction displayed higher ferrous ion chelating (EC50 = 1 mg mL(-1)). Cytotoxicity evaluation of crude extract and total saponin fraction against MCF-7, PC3, and HCT-116 cancer cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) cell viability assay indicated a higher cytotoxicity activity of the crude extract than the total saponin fraction on all cell lines, being most effective and selective on MCF-7 human breast cancer cell line.
Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia ; School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
Mehdi Farshad
MF
Ashraf
Maheran
M
Abd Aziz
Johnson
J
Stanslas
Ismanizan
I
Ismail
Mihdzar
M
Abdul Kadir
D000970:Antineoplastic Agents / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D045424:Complex Mixtures / Q000633:toxicity*; D016166:Free Radical Scavengers / Q000633:toxicity*; D006801:Humans; D061986:MCF-7 Cells; D019684:Magnoliopsida / Q000737:chemistry*; D010936:Plant Extracts / Q000633:toxicity*; D012503:Saponins / Q000633:toxicity*
D000970:Antineoplastic Agents; D045424:Complex Mixtures; D016166:Free Radical Scavengers; D010936:Plant Extracts; D012503:Saponins