Metabolism of nonesterified and esterified hydroxycinnamic acids in red wines by Brettanomyces bruxellensis.
While Brettanomyces can metabolize nonesterified hydroxycinnamic acids found in grape musts/wines (caffeic, p-coumaric, and ferulic acids), it was not known whether this yeast could utilize the corresponding tartaric acid esters (caftaric, p-coutaric, and fertaric acids, respectively). Red wines from Washington and Oregon were inoculated with B. bruxellensis, while hydroxycinnamic acids were monitored by HPLC. Besides consuming p-coumaric and ferulic acids, strains I1a, B1b, and E1 isolated from Washington wines metabolized 40-50% of caffeic acid, a finding in contrast to strains obtained from California wines. Higher molar recoveries of 4-ethylphenol and 4-ethylguaiacol synthesized from p-coumaric and ferulic acids, respectively, were observed in Washington Cabernet Sauvignon and Syrah but not Merlot. This finding suggested that Brettanomyces either (a) utilized vinylphenols formed during processing of some wines or (b) metabolized other unidentified phenolic precursors. None of the strains of Brettanomyces studied metabolized caftaric or p-coutaric acids present in wines from Washington or Oregon.
School of Food Science, Washington State University , Pullman, Washington 99164-6376, United States.
Lauren M
LM
Schopp
Jungmin
J
Lee
James P
JP
Osborne
Stuart C
SC
Chescheir
Charles G
CG
Edwards
D055168:Brettanomyces / Q000378:metabolism*; D002140:California; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000737:chemistry / Q000378:metabolism*; D004951:Esterification; D006139:Guaiacol / Q000031:analogs & derivatives / Q000378:metabolism; D009922:Oregon; D010636:Phenols / Q000378:metabolism; D014861:Washington; D014920:Wine / Q000382:microbiology*
D003373:Coumaric Acids; D010636:Phenols; C515102:coutaric acid; D006139:Guaiacol; C042291:4-ethylphenol; C004999:ferulic acid; C084991:4-ethylguaiacol