The effect of gamma radiation on the lipid profile of irradiated red blood cells.
An investigation into the effects of irradiation and of the storage time on aging and quality are a relevant issue to ensure the safety and the efficiency of irradiation in the prevention of transfusion-associated graft-versus-host disease (TA-GVHD). In this work, the biochemical properties and alterations presented by erythrocyte membranes, up to 28-days post-irradiation, with a dose of 25 Gy, were studied as a function of storage and post-irradiation time. There was a considerable variation in the total of phospholipid content, when comparing the control and irradiated samples, mostly from the third day onwards; and at the same time, the effect occurred as a function on the storage time of blood bags. The levels of total cholesterol decreased 3-9 days after irradiation. TBARS levels were increased after irradiation and 7 days of storage, but no increment of catalase activity was observed after the irradiation. Furthermore, the protein profile was maintained throughout the irradiation and storage time, until the 21st day, with the presence of a protein fragmentation band of around 28 kDa on the 28th day. In conclusion, although gamma irradiation is the main agent for the prevention of TA-GVHD, a better understanding of the physical and biochemical properties of erythrocytes are necessary to better assess their viability, and to be able to issue more secure recommendations on the shelf life of blood bags, and the safe use of the irradiated red cells therein.
Faculdade de Bioquímica, Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal de São João del Rei, Campus Centro-Oeste Dona Lindú, Av Sebastião Gonçalves Coelho, 400, Bairro Chanadour, CEP, 35501-296, Divinópolis, Minas Gerais, Brazil.
Grazielle Aparecida Silva
GA
Maia
Cristiane de Oliveira
Cde O
Renó
Jorge Mansur
JM
Medina
Alan Barbosa da
AB
Silveira
Julio Alberto
JA
Mignaco
Georgia Correa
GC
Atella
Vanessa Faria
VF
Cortes
Leandro Augusto
LA
Barbosa
Hérica de Lima
Hde L
Santos
D001793:Blood Preservation; D002374:Catalase / Q000378:metabolism; D002784:Cholesterol / Q000737:chemistry*; D004307:Dose-Response Relationship, Radiation; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D004912:Erythrocytes / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D005720:Gamma Rays; D015227:Lipid Peroxidation / Q000528:radiation effects; D008563:Membrane Lipids / Q000737:chemistry; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000737:chemistry*; D059748:Proteolysis; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D008563:Membrane Lipids; D010743:Phospholipids; D017392:Thiobarbituric Acid Reactive Substances; D002784:Cholesterol; D002374:Catalase