Incongruence between multi-locus sequence analysis (MLSA) and whole-genome-based phylogenies: Pseudomonas syringae pathovar pisi as a cautionary tale.
Previous phylogenies, built using a subset of genomic loci, split Pseudomonas syringae pv. pisi into two well-supported clades and implied convergence in host range for these lineages. The analysis of phenotypic and genotypic data within the context of this phylogenetic relationship implied further convergence at the level of virulence gene loss and acquisition. We generate draft genome assemblies for two additional P. syringae strains, isolated from diseased pea plants, and demonstrate incongruence between phylogenies created from a subset of the data compared with the whole genomes. Our whole-genome analysis demonstrates that strains classified as pv. pisi actually form a coherent monophyletic clade, so that apparent convergence is actually the product of shared ancestry. We use this example to urge caution when making evolutionary inferences across closely related strains of P. syringae.
School of Plant Sciences, University of Arizona, Tucson, AZ, 85721-0036, USA.
David A
DA
Baltrus
Kevin
K
Dougherty
Stephen M
SM
Beckstrom-Sternberg
James S
JS
Beckstrom-Sternberg
Jeffrey T
JT
Foster
D016680:Genome, Bacterial / Q000235:genetics; D010802:Phylogeny; D044224:Pseudomonas syringae / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D017422:Sequence Analysis, DNA; D014774:Virulence / Q000235:genetics