Measurement of Electron Transport System (ETS) activity in soil.
Electron transport system (ETS) activity was measured in amended and nonamended soil by measuring the reduction of 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride (INT) to iodonitrotetrazolium formazan (INT-formazan), which can be easily extracted with methanol without interference from other compounds found in soil. A high correlation between ETS activity and oxygen consumption was observed. This technique allows rapid quantitative measurements of microbial ETS activity in soil.
Department of Biology, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada.
J T
JT
Trevors
C I
CI
Mayfield
W E
WE
Inniss