Effects of Pluronic F-68 on callus growth and protoplast plating efficiency of Solanum dulcamara.
The effects of the non-ionic surfactant, Pluronic F-68, on the growth of callus and protoplasts from Solanum dulcamara L. have been studied. Growth of callus was stimulated by addition of 0.1% (w/v) commercial grade Pluronic to culture medium, whereas lower concentrations (0.01% w/v) had no corresponding effect. In contrast, higher concentrations (1.0% w/v) of Pluronic inhibited callus growth. The mean plating efficiency of protoplasts grown at different densities (15 days after plating) was increased up to 26% following culture with 0.1% (w/v) Pluronic, while 0.01% (w/v) Pluronic was ineffective. Mean protoplast plating efficiency decreased by up to 32% following culture with 1.0% (w/v) Pluronic.
Departments of Botany, University of Nottingham, NG7 2RD, University Park, Nottingham, UK.
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V
Kumar
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Laouar
M R
MR
Davey
B J
BJ
Mulligan
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Lowe