Delayed luminescence of luminol initiated by a membrane-bound peroxidase.
The luminescense of the luminol-H2O2 system was initiated by either free or membrane-bound horseradish peroxideae (HRP). The instantaneous luminescene decayed rapidly and was followed by the delayed luminescence in the presence of excess luminol. The delayed luminescence was characterized by a chain reaction, in which luminescence intensity increased exponentially. Membrane-bound HRP demonstrated that the delayed luminescence took place even in the absence of HRP if the instantaneous luminescence was initiated by HRP. A mechanism for the nonenzymatic luminescence is proposed and discussed.
Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Nagatsuta, Midori-ku, 227, Yokohama, Japan.
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Y
Ikariyama
S
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Suzuki
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Aizawa