Paenibacillus selenitireducens sp. nov., a selenite-reducing bacterium isolated from a selenium mineral soil.
A Gram-stain-positive, rod-shaped, facultatively anaerobic bacterium, designated strain ES3-24(T), was isolated from a selenium mineral soil. The isolate was endospore-forming, nitrate-reducing and motile by means of peritrichous flagella. The major menaquinone was menaquinone 7 (MK-7) and the predominant fatty acids (>5%) were anteiso-C15:0, iso-C16:0, C16:0 and anteiso-C17:0. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and two unknown aminophospholipids. Strain ES3-24(T) contained meso-diaminopimelic acid in the cell-wall peptidoglycan and the DNA G+C content was 49.6 mol%. According to phylogenetic analysis based on the 16S rRNA gene sequence, strain ES3-24(T) was most closely related to Paenibacillus terrigena A35(T), with 16S rRNA gene sequence identity of 98.3%, while the other members of the genus Paenibacillus had 16S rRNA gene sequence identities of less than 95.0%. DNA-DNA relatedness between strain ES3-24(T) and P. terrigena CCTCC AB206026(T) was 39.3 %. In addition, strain ES3-24(T) showed obvious differences from closely related species in major polar lipids, nitrate reduction and other physiological and biochemical characteristics. The data from our polyphasic taxonomic study reveal that strain ES3-24(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus selenitireducens sp. nov. is proposed. The type strain is ES3-24(T) ( = KCTC 33157(T) = CCTCC AB2013097(T)).
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.
Rong
R
Yao
Tobacco Company of Enshi, Hubei Province, Enshi, 445000, Hubei, PR China.|State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.
Rui
R
Wang
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.
Dan
D
Wang
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.
Jing
J
Su
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.
Shixue
S
Zheng
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.
Gejiao
G
Wang
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D056507:Paenibacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012643:Selenium; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7; D012643:Selenium