DNA extraction from vegetative tissue for next-generation sequencing.
The quality of extracted DNA is crucial for several applications in molecular biology. If the DNA is to be used for next-generation sequencing (NGS), then microgram quantities of good-quality DNA is required. In addition, the DNA must substantially be of high molecular weight so that it can be used for library preparation and NGS sequencing. Contaminating phenol or starch in the isolated DNA can be easily removed by filtration through kit-based cartridges. In this chapter we describe a simple two-reagent DNA extraction protocol which yields a high quality and quantity of DNA which can be used for different applications including NGS.
Centre for Nutrition and Food Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD, Australia.
Agnelo
A
Furtado
D018744:DNA, Plant / Q000737:chemistry / Q000302:isolation & purification*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D010945:Plants, Edible / Q000737:chemistry* / Q000235:genetics
D018744:DNA, Plant