Highly efficient phase boundary biocatalysis with enzymogel nanoparticles.
The enzymogel nanoparticle made of a magnetic core and polymer brush shell demonstrates a novel type of remote controlled phase-boundary biocatalysis that involves remotely directed binding to and engulfing insoluble substrates, high mobility, and stability of the catalytic centers. The mobile enzymes reside in the polymer brush scaffold and shuttle between the enzymogel interior and surface of the engulfed substrate in the bioconversion process. Biocatalytic activity of the mobile enzymes is preserved in the enzymogel while the brush-like architecture favors the efficient interfacial interaction when the enzymogel spreads over the substrate and extends substantially the reaction area as compared with rigid particles.
Department of Coatings and Polymeric Materials, North Dakota State University, Fargo, ND 58108 (USA).
Olena
O
Kudina
Andrey
A
Zakharchenko
Oleksandr
O
Trotsenko
Alexander
A
Tokarev
Leonid
L
Ionov
Georgi
G
Stoychev
Nikolay
N
Puretskiy
Scott W
SW
Pryor
Andriy
A
Voronov
Sergiy
S
Minko
D000180:Acrylic Resins / Q000737:chemistry; D055162:Biocatalysis; D002480:Cellulase / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D005782:Gels / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D060526:Magnetic Fields; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry*; D009994:Osmolar Concentration; D010316:Particle Size; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties
D000180:Acrylic Resins; D004800:Enzymes, Immobilized; D005456:Fluorescent Dyes; D005782:Gels; C006903:carbopol 940; D012822:Silicon Dioxide; D002480:Cellulase
bioconversion; enzymatic catalysis; nanoparticles; polymer brushes; stimuli-responsive materials