Cytotoxic effect of chlorpyrifos ethyl and its degradation derivatives by Pseudomonas peli strain isolated from the Oued Hamdoun River (Tunisia).
A bacterium was isolated from the river of Oued Hamdoun (Tunisia), and its phenotypic features, physiological and chemotaxonomic characteristics and phylogenetic analysis of 16S ribosomal RNA sequence revealed it as Pseudomonas peli (P. peli). Chlorpyrifos ethyl (CP) was used as the sole source of carbon and energy by P. peli, and it was cometabolised in the presence of glucose. CP was completely degraded by P. peli after 96 h of shake incubation. High-performance liquid chromatography analysis indicated that the biodegradation kinetics was not affected by the addition of glucose into the culture medium. In the present study, only transient accumulation of one major no-identified product was observed after 48 h of incubation, with no other persistent metabolites detected. Cytotoxicity of CP, before and after biodegradation with P. peli, was evaluated in vitro using the MTT-colorimetric assay against three human cancer cell lines (A549, lung cell carcinoma, HT29, colon adenocarcinoma and MCF7, breast adenocarcinoma). CP reduced viability of all human cell lines in a dose-dependent manner. Its activity was very remarkable against A549 cell line. However, cytotoxicity strongly decreased in CP obtained after incubation with P. peli Hence, we conclude that when incubated under appropriate conditions,P. peli has a metabolism that completely detoxifies CP.
Laboratoire de Biotechnologie et Valorisation de Bio Géo Ressources, Institut Supérieur de Biotechnologie (LBVBGR-LR11-ES31), BiotechPole de Sidi Thabet, Université de la Manouba, Manouba, Tunisie Laboratoire de Pharmacologie des Médicaments Anticancéreux, Institut Bergonie, Université Victor Segalen, Bordeaux, France.
Afef
A
Dellai
Laboratoire de Biotechnologie et Valorisation de Bio Géo Ressources, Institut Supérieur de Biotechnologie (LBVBGR-LR11-ES31), BiotechPole de Sidi Thabet, Université de la Manouba, Manouba, Tunisie.
Dorra
D
Dridi
Laboratoire de Biotechnologie et Valorisation de Bio Géo Ressources, Institut Supérieur de Biotechnologie (LBVBGR-LR11-ES31), BiotechPole de Sidi Thabet, Université de la Manouba, Manouba, Tunisie.
Seif
S
Sakouhi
Laboratoire de Pharmacologie des Médicaments Anticancéreux, Institut Bergonie, Université Victor Segalen, Bordeaux, France.
Jacques
J
Robert
Ecole des Métiers de l'Environnement, Université Européenne de Bretagne, Rennes, France.
Hayet
H
Djelal
Laboratoire des Aliments Bioprocédés, Toxicologie, Environ-nements (ABTE-EA 4651), IUT de Caen, Université de Caen Basse-Normandie, Caen, France.
Ridha
R
Mosrati
Laboratoire de Biotechnologie et Valorisation de Bio Géo Ressources, Institut Supérieur de Biotechnologie (LBVBGR-LR11-ES31), BiotechPole de Sidi Thabet, Université de la Manouba, Manouba, Tunisie.
Ameur
A
Cherif
Laboratoire de Biotechnologie et Valorisation de Bio Géo Ressources, Institut Supérieur de Biotechnologie (LBVBGR-LR11-ES31), BiotechPole de Sidi Thabet, Université de la Manouba, Manouba, Tunisie Laboratoire de Pharmacologie des Médicaments Anticancéreux, Institut Bergonie, Université Victor Segalen, Bordeaux, France hdbenmansour@gmail.com.
Hedi Ben
HB
Mansour
D001673:Biodegradation, Environmental; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004390:Chlorpyrifos / Q000378:metabolism / Q000633:toxicity; D006801:Humans; D011549:Pseudomonas / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D045483:Rivers / Q000382:microbiology; D014416:Tunisia; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity
D014874:Water Pollutants, Chemical; D004390:Chlorpyrifos
HPLC; Pseudomonas peli; biodegradation; chlorpyrifos ethyl; cytotoxicity