Differential stress transcriptome landscape of historic and recently emerged hypervirulent strains of Clostridium difficile strains determined using RNA-seq.
C. difficile is the most common cause of nosocomial diarrhea in North America and Europe. Genomes of individual strains of C. difficile are highly divergent. To determine how divergent strains respond to environmental changes, the transcriptomes of two historic and two recently isolated hypervirulent strains were analyzed following nutrient shift and osmotic shock. Illumina based RNA-seq was used to sequence these transcriptomes. Our results reveal that although C. difficile strains contain a large number of shared and strain specific genes, the majority of the differentially expressed genes were core genes. We also detected a number of transcriptionally active regions that were not part of the primary genome annotation. Some of these are likely to be small regulatory RNAs.
Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, New York, United States of America.
Joy
J
Scaria
Chunhong
C
Mao
Jenn-Wei
JW
Chen
Sean P
SP
McDonough
Bruno
B
Sobral
Yung-Fu
YF
Chang
D016360:Clostridioides difficile / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D021821:Communicable Diseases, Emerging / Q000235:genetics / Q000378:metabolism; D004761:Enterocolitis, Pseudomembranous / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D006801:Humans; D009997:Osmotic Pressure; D012329:RNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D013045:Species Specificity; D059467:Transcriptome
D012329:RNA, Bacterial