The behaviour of tomato golden mosaic virus DNA in cultured cells isolated from systemically infected tobacco leaves.
When callus tissue was cultured from leaf pieces taken from a Nicotiana tabacum cv. Xanthi nc. plant systemically infected with tomato golden mosaic virus (TGMV), TGMV-specific DNA persisted for up to 6 months in culture. Analysis of TGMV-specific intracellular DNA forms indicated a decrease in double-stranded relative to single-stranded forms and an increase in sub-genomic relative to genomic single-stranded DNA species in the callus tissue compared to those in the original leaf explant. The implications of the results with regard to TGMV replication are discussed.
Department of Pure and Applied Biology, Imperial College of Science and Technology, Prince Consort Road, SW7 2BB, London, UK.
M J
MJ
Slomka
K W
KW
Buck
R H
RH
Coutts