An apolipoprotein E mimetic peptide with activities against multidrug-resistant bacteria and immunomodulatory effects.
Apolipoprotein E (apoE) mimetic peptides derived from the low-density lipoprotein receptor-binding region of apoE with both activities against multidrug-resistant bacteria and immunomodulatory effects have not previously been reported. We identified an apoE mimetic peptide analogue of the receptor-binding region of apoE (abbreviated as apoE23) with the sequence of LRKLRKRLVRLASHLRKLRKRLL, which exhibited high antibacterial effects. The minimal inhibitory concentration of apoE23 against multidrug-resistant Acinetobacter baumannii was 6 µg/ml. The antimicrobial activity of apoE23 depended on its amphipathic α-helical conformation. Moreover, apoE23 downregulated the expression of tumour necrosis factor-α, interleukin-6 and interleukin-10 in lipopolysaccharide-induced THP-1 cells. ApoE23 exhibits potential in future clinical applications.
The Clinical Microbiology Lab, Department of Nosocomial Infection Control, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai, 201102, China.
Chuan-qing
CQ
Wang
Chang-sheng
CS
Yang
Yi
Y
Yang
Fu
F
Pan
Lei-yan
LY
He
Ai-min
AM
Wang
D040981:Acinetobacter baumannii / Q000187:drug effects*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001057:Apolipoproteins E / Q000737:chemistry*; D002460:Cell Line; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D007155:Immunologic Factors / Q000737:chemistry / Q000494:pharmacology*; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D018716:Molecular Mimicry; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D017433:Protein Structure, Secondary; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001057:Apolipoproteins E; C508609:IL10 protein, human; C508600:IL6 protein, human; D007155:Immunologic Factors; D015850:Interleukin-6; D008070:Lipopolysaccharides; D010455:Peptides; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10
antibacterial peptide; apolipoprotein E; immunomodulation; mimetic peptide