The effect of gelling agents on plaunotol accumulation in callus cultures ofCroton sublyratus Kurz.
Callus cultures established from the leaves ofCroton sublyratus Kurz (Euphorbiaceae) contained little or no plaunotol on various media with different hormone combinations. Plaunotol accumulation was observed in calli which were cultured in media with increasing concentrations of gelling agents, especially gellan gum and agarose. The accumulation was observed within three weeks after transfer to the medium and was accompanied by chlorophyll increase, tracheid development and slow growth. Light and increasing concentration of gelling agents were observed to be indispensable for plaunotol accumulation in the callus.
Plant Laboratory, Kirin Brewery Co., Ltd., 3377 Kitsuregawa-machi, Shioya-gun, Tochigi-ken, Japan.
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Morimoto
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Murai