Plant regeneration from pea protoplasts via somatic embyogenesis.
Plant regeneration via somatic embryogenesis was obtained from pea protoplasts. Strong auxins (picloram or 2.4-D) and increased osmolarity of the medium were necessary for embryo induction. Relatively high amounts of embryogenic calli could be obtained in 2 genotypes. After a period on hormone-free medium, a second induction of somatic embryos was possible. Further development of somatic embryos was accomplished on GA3 - containing medium.
Institut für Genetik, Universität Bonn, Kirschallee 1, D-5300, Bonn 1, Federal Republic of Germany.
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Lehminger-Mertens
H J
HJ
Jacobsen