Magnetic biospecific affinity adsorbents for immunoglobulin and enzyme isolation.
Magnetic biospecific affinity adsorbents for immunoglobulin and enzyme isolation have been prepared. They were obtained by a " post-magnetization" procedure involving a simple treatment of the various affinity gels with magnetic ferrofluid. The magnetic biospecific adsorbents tested include magnetic protein A-Sepharose for isolation of IgG antibodies, magnetic human serum albumin (HSA)-Sepharose for anti-HSA isolation, and magnetic 2',5'-ADP for isolation of glucose-6-phosphate dehydrogenase from baker's yeast and hemolyzates of human red blood cells. For the latter enzyme, a 11,000-fold purification was achieved in one step.
Pure and Applied Biochemistry, Chemical Center, University of Lund, PO Box 740, S-220 07, Lund, Sweden.
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Griffin
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Mosbach
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Mosbach