Genetic control and linkage relations of additional isozyme markers in chick-pea.
Allozyme polymorphisms of nine enzymes - aspartate aminotransferase (AAT), diaphorase (DIA), esterase (EST), formate dehydrogenase (FDH), β-galactosidase (GAL), β-glucosidase (GLU), malate dehydrogenase (MDH), malic enzyme (ME), and peroxidase (PRX) - were described in chick-pea (Cicer L.). Thirteen isozyme loci, Aat-c, Dia-4, Est-2, Est-4, Est-10, Fdh, Gal-2, Gal-3, Gal-4, Glu-3, Mdh-2, Me-2, and Prx-2, were genetically defined. Alleles of each of these isozyme loci expressed codominantly in heterozygotes and exhibited a codominant, single-locus segregation ratio in F2. The loci Est-2, Mdh-2, and Me-1 were expressed only in flower. Linkage relations were determined for these 13 and several previously defined isozyme loci. The following new genetic linkages were identified: Pgm-p (locus for plastid phosphoglucomutase) - Est-10; Ald-p1 (one of the duplicate loci for plastid aldolase) - Glu-3 - Gal-2 - Est-2,3; Gal-3 - Aco-m (locus for mitochondrial aconitase) - Prx-2,3; Gpi-c (locus for cytosolic glucosephosphate isomerase) - Fdh; and Est-4 - Me-1. This study provides further confirmation on the existence of several conserved linkage groups among Cicer, Pisum, and Lens.
Department of Crop Science and Plant Ecology, University of Saskatchewan, S7N 0W0, Saskatoon, Sask, Canada.
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Gaur
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Slinkard