Purification of two triterpenoids from Schisandra chinensis by macroporous resin combined with high-speed counter-current chromatography.
A method for preparative purification of corosolic acid and nigranoic acid from Schisandra chinensis (SC) was established using a combination of macroporous absorption resin column separation and high-speed counter-current chromatography (HSCCC). The crude extracts obtained from SC using 70% ethanol were separated on a macroporous resin column and then eluted with a graded ethanol series. The 70% ethanol fraction was used as the sample for separation of the two triterpenoids by HSCCC. The two-phase solvent system used for HSCCC separation was chloroform-n-butanol-methanol-water (10:0.5:7:4, v/v/v/v). The upper phase was used as the stationary phase of HSCCC. Corosolic acid (16.4 mg) of 96.3% purity and nigranoic acid (9.5 mg) of 98.9% purity were obtained in a one-step HSCCC separation from 100 mg of the sample. The structures of corosolic acid and nigranoic acid were identified by (1)H-nuclear magnetic resonance (NMR) and (13)C-NMR.
College of Food, Shenyang Agricultural University, Shenyang 110866, People's Republic of China.
Lijie
L
Zhu
College of Food, Shenyang Agricultural University, Shenyang 110866, People's Republic of China.
Bin
B
Li
College of Food, Shenyang Agricultural University, Shenyang 110866, People's Republic of China.
Xiuying
X
Liu
College of Food, Shenyang Agricultural University, Shenyang 110866, People's Republic of China mengxjsy@126.com.
Xianjun
X
Meng
D020001:1-Butanol / Q000737:chemistry; D002725:Chloroform / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods*; D003627:Data Interpretation, Statistical; D004867:Equipment Design; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol / Q000737:chemistry; D015394:Molecular Structure; D015203:Reproducibility of Results; D012117:Resins, Synthetic / Q000737:chemistry*; D032141:Schisandra / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D014315:Triterpenes / Q000302:isolation & purification*
D012117:Resins, Synthetic; D012997:Solvents; D014315:Triterpenes; C100860:nigranoic acid; D002725:Chloroform; D020001:1-Butanol; C113861:corosolic acid; D000432:Methanol