A novel osmotic pressure control fed-batch fermentation strategy for improvement of erythritol production by Yarrowia lipolytica from glycerol.
The effect of osmotic pressure on erythritol and mannitol production by an osmophilic yeast strain of Yarrowia lipolytica CICC 1675 using glycerol as the sole carbon source was investigated. Appropriately high osmotic pressure was found to enhance erythritol production and inhibit mannitol formation. A novel two-stage osmotic pressure control fed-batch strategy based on the kinetic analysis was developed for higher erythritol yield and productivity. During the first 96 h, the osmotic pressure was maintained at 4.25 osmol/kg by feeding glycerol to reduce the inhibition of cell growth. After 132 h, the osmotic pressure was controlled at 4.94 osmol/kg to maintain a high dp(ery)/dt. Maximum erythritol yield of 194.3g/L was obtained with 0.95 g/L/h productivity, which were 25.7% and 2.2%, respectively, improvement over the best results in one-stage fed-batch fermentation. This is the first report that a novel osmotic pressure control fed-batch strategy significantly enhanced erythritol production.
Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China.
Li-Bo
LB
Yang
Xiao-Bei
XB
Zhan
Zhi-Yong
ZY
Zheng
Jian-Rong
JR
Wu
Min-Jie
MJ
Gao
Chi-Chung
CC
Lin
D061249:Batch Cell Culture Techniques / Q000379:methods*; D018533:Biomass; D004896:Erythritol / Q000096:biosynthesis*; D005285:Fermentation / Q000187:drug effects; D005990:Glycerol / Q000378:metabolism*; D008353:Mannitol / Q000378:metabolism; D009997:Osmotic Pressure / Q000187:drug effects; D011108:Polymers / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013997:Time Factors; D025062:Yarrowia / Q000187:drug effects / Q000378:metabolism*
D011108:Polymers; C024617:polyol; D008353:Mannitol; D012965:Sodium Chloride; D005990:Glycerol; D004896:Erythritol
Erythritol; Fed-batch; High productivity; Osmotic pressure control strategy; Yarrowia lipolytica