Electron Ionization-Induced Release of Coded Isotopic Reporter Ions in anm/zZone of Minimal Interference for Quantifiable, Multiplexed GC-MS Analyses.
We describe an isotope coding strategy that enables simultaneous GC-MS analysis of multiple samples for substrate identification and quantification. The method relies on direct measurement of isotopic ethyl carbenium ions serving as mass spectral tags in a zone of minimal interference (ZMI) at m/z 31-37. Sample aldehyde and ketone mixtures were reacted with isotopic 2-aminooxyethyl propionates to illustrate the method, which determined the relative abundance of the mixed compounds with an average 95% accuracy. ZMI reporter ion detection also enables chemoselective substrate profiling and absolute quantification, as demonstrated using a biologically derived sample.
University of Louisville, Department of Chemistry, 2320 South Brook Street, Louisville, Kentucky 40292, United States. Tel: 01 502 852 8069.
Sébastien
S
Laulhé
Tyler E
TE
Geers
Xue
X
Shi
Xiang
X
Zhang
Michael H
MH
Nantz