Multiplexed dual second-dimension column comprehensive two-dimensional gas chromatography (GC × 2GC) using thermal modulation and contra-directional second-dimension columns.
A multiplexed dual-secondary column comprehensive two-dimensional gas chromatography approach (GC×2GC) designed for complex sample analysis is introduced. The approach splits the first-dimension column effluent into two second-dimension columns with different stationary phases, and recombines the two streams into one detector post-separation. The approach produces two single two-dimensional chromatograms for each injection. Careful manipulation of thermal modulator timing parameters combined with a novel contra-directional modulation regime facilitates this approach. A selection of 34 laboratory reference compounds containing n-alkanes, alcohols, aromatic hydrocarbons, ketones, esters and halogenated hydrocarbons were analysed to demonstrate the approach. The dual two-dimensional chromatogram from this single detector system provides complementary information due to the unique selectivity of the three separation columns. The results of this proof-of-principle investigation provide significant impetus for further development of GC×2GC-MS methodology.
Australian Centre for Research on Separation Science (ACROSS), University of Tasmania, Private Bag 75, Hobart 7001, Australia.
Benjamin
B
Savareear
Robert A
RA
Shellie
D000438:Alcohols / Q000032:analysis; D000473:Alkanes / Q000032:analysis; D002849:Chromatography, Gas / Q000295:instrumentation*; D004867:Equipment Design; D006841:Hydrocarbons, Aromatic / Q000032:analysis; D006846:Hydrocarbons, Halogenated / Q000032:analysis; D007659:Ketones / Q000032:analysis; D013696:Temperature
D000438:Alcohols; D000473:Alkanes; D006841:Hydrocarbons, Aromatic; D006846:Hydrocarbons, Halogenated; D007659:Ketones
Comprehensive two-dimensional; Contra-directional; Dual second-dimension columns; Gas chromatography; Multiplexed