Centrosome-dependent asymmetric inheritance of the midbody ring in Drosophila germline stem cell division.
Many stem cells, including Drosophila germline stem cells (GSCs), divide asymmetrically, producing one stem cell and one differentiating daughter. Cytokinesis is often asymmetric, in that only one daughter cell inherits the midbody ring (MR) upon completion of abscission even in apparently symmetrically dividing cells. However, whether the asymmetry in cytokinesis correlates with cell fate or has functional relevance has been poorly explored. Here we show that the MR is asymmetrically segregated during GSC divisions in a centrosome age-dependent manner: male GSCs, which inherit the mother centrosome, exclude the MR, whereas female GSCs, which we here show inherit the daughter centrosome, inherit the MR. We further show that stem cell identity correlates with the mode of MR inheritance. Together our data suggest that the MR does not inherently dictate stem cell identity, although its stereotypical inheritance is under the control of stemness and potentially provides a platform for asymmetric segregation of certain factors.
Life Sciences Institute, Center for Stem Cell Biology, University of Michigan, Ann Arbor, MI 48109 Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109 Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109 Department of Cellular and Molecular Biology, University of Michigan, Ann Arbor, MI 48109.
Viktoria
V
Salzmann
Cuie
C
Chen
C-Y Ason
CY
Chiang
Amita
A
Tiyaboonchai
Michael
M
Mayer
Yukiko M
YM
Yamashita
D053687:Adult Stem Cells / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002455:Cell Division / Q000235:genetics; D018385:Centrosome; D020090:Chromosome Segregation; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development; D008941:Spindle Apparatus / Q000235:genetics