Plant regeneration through somatic embryogenesis from suspension cultures of a minor millet, Paspalum scrobiculatum.
Compact, friable and embryogenic calli were initiated from immature inflorescences and young leaf bases of one week old seedlings of Paspalum scrobiculatum cultured on MS medium supplemented with 2,4-D. A stable, embryogenic suspension culture was initiated from these calli and maintained in a liquid version of the same MS medium. Embryogenic calli and somatic embryos were obtained by plating suspension culture cells onto semi-solid medium containing 2,4-D. Complete, normal plantlets developed on 2,4-D free medium at a high frequency from somatic embryos. NAA and BAP in the medium promoted plant development.
Programme in Genetical Research, Bose Institute Centenary Building, P1/12, C.I.T. Scheme VII-M, 700 054, Calcutta, India.
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