Adoptive immunotherapy: New assay for the identification of T cells with optimal avidity.
T cells expressing high avidity T-cell receptors (TCRs) have been shown to mediate superior therapeutic effects. A novel koff-rate assay allows for the quantitative and reproducible assessment of the avidity of TCRs for their ligands directly on living T cells, ex vivo.This assay might facilitate the selection of T cells with an optimal avidity for their target, hence favoring the development of adoptive immunotherapeutic regimens.
Institute for Medical Microbiology; Immunology and Hygiene; Technische Universität München; Munich, Germany.
Bianca
B
Weissbrich
Magdalena
M
Nauerth
Dirk H
DH
Busch
CD8+ cytotoxic T cell; Koff rate; T cell; T cell receptor; avidity