Target-induced quenching for highly sensitive detection of nucleic acids based on label-free luminescent supersandwich DNA/silver nanoclusters.
Luminescent silver nanoclusters (AgNCs) were anchored by designed oligonucleotides, acting as fluorescent labels. They hybridized with specific nucleic acid targets to form a supersandwich structure resulting in the fluorescence intensity of the DNA/AgNCs decreasing linearly with respect to the concentration of the target DNA.
Key Laboratory of Chem-Biosensing, College of Chemistry and Materials Science, Center for Nano Science and Technology, Anhui Normal University, Wuhu 241000, Anhui Province, P.R. China. xjzhang@mail.ahnu.edu.cn.
Guangfeng
G
Wang
Yanhong
Y
Zhu
Ling
L
Chen
Lun
L
Wang
Xiaojun
X
Zhang
D004247:DNA / Q000737:chemistry*; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods* / Q000592:standards; D053768:Metal Nanoparticles / Q000737:chemistry*; D009696:Nucleic Acids / Q000032:analysis*; D012834:Silver / Q000737:chemistry*
D009696:Nucleic Acids; D012834:Silver; D004247:DNA