Measuring calcium uptake and inositol 1,4,5-trisphosphate-induced calcium release in cerebellum microsomes using Fluo-3.
A simple fluorimetric method is presented for measuring calcium uptake and inositol 1,4,5-trisphosphate (InsP3)-induced calcium release in microsomes using Fluo-3. This method is used to investigate the amplitude of calcium release (quantal release) induced by InsP3 from rat cerebellum microsomes.
School of Biochemistry, University of Birmingham, Edgbaston, B15 2TT, Birmingham, UK.
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Michelangeli