Protein detection through different platforms of immuno-loop-mediated isothermal amplification.
Different immunoassay-based methods have been devised to detect protein targets. These methods have some challenges that make them inefficient for assaying ultra-low-amounted proteins. ELISA, iPCR, iRCA, and iNASBA are the common immunoassay-based methods of protein detection, each of which has specific and common technical challenges making it necessary to introduce a novel method in order to avoid their problems for detection of target proteins. Here we propose a new method nominated as 'immuno-loop-mediated isothermal amplification' or 'iLAMP'. This new method is free from the problems of the previous methods and has significant advantages over them. In this paper we also offer various configurations in order to improve the applicability of this method in real-world sample analyses. Important potential applications of this method are stated as well.
Mohammad
M
Pourhassan-Moghaddam
Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz 51656, Iran. rahmatimo@tbzmed.ac.ir.
Mohammad
M
Rahmati-Yamchi
Abolfazl
A
Akbarzadeh
Hadis
H
Daraee
Kazem
K
Nejati-Koshki
Younes
Y
Hanifehpour
Sang Woo
SW
Joo