Brain norepinephrine identified by mass spectrometry is associated with reproductive status of females of the linden bug Pyrrhocoris apterus.
Several biogenic amines, including controversial presence of norepinephrine (NE), were identified by the high performance liquid chromatography equipped with electrospray ionisation mass spectrometry in brain complexes of adult females of Pyrrhocoris apterus. Quantitative analysis was performed by the high performance liquid chromatography coupled to electrochemical detector. Levels of NE, dopamine (DA), octopamine (OA) and 5-hydroxytryptamine (5-HT) in brain complexes were measured in reproductive vs. diapause females. In field collected samples, levels of NE and DA were significantly higher in reproductive (May) than in non-reproductive (Sep, Oct, Feb) females. In laboratory females, NE is higher in long day photoperiod (reproduction) than in short day photoperiod (diapause) already from the first week of the adult age, while DA shows differences between the two contrasting photoperiods only from the second week of the adult age. No association between reproductive status and levels of OA and 5-HT was found.
Department of Applied Chemistry, Faculty of Agriculture, University of South Bohemia, 370 05 České Budějovice, Czech Republic; Institute of Entomology, Biology Center, Academy of Sciences of the Czech Republic, 37005 České Budějovice, Czech Republic.
Daniela
D
Chvalova
Institute of Entomology, Biology Center, Academy of Sciences of the Czech Republic, 37005 České Budějovice, Czech Republic.
Lenka
L
Zdechovanova
Institute of Entomology, Biology Center, Academy of Sciences of the Czech Republic, 37005 České Budějovice, Czech Republic.
Hana
H
Vaneckova
Institute of Entomology, Biology Center, Academy of Sciences of the Czech Republic, 37005 České Budějovice, Czech Republic. Electronic address: magda@entu.cas.cz.
Magdalena
M
Hodkova
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002395:Catecholamines / Q000378:metabolism; D002853:Chromatography, Liquid; D004298:Dopamine / Q000378:metabolism; D005260:Female; D020062:Heteroptera / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism*; D009655:Octopamine / Q000378:metabolism; D012098:Reproduction; D012621:Seasons; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D032384:Tilia / Q000469:parasitology*
D002395:Catecholamines; D009655:Octopamine; D004298:Dopamine; D009638:Norepinephrine
Adult diapause; Brain complex; Dopamine; Insects; Mass spectrometry; Norepinephrine