Genetic assessment of common bean (Phaseolus vulgaris L.) accessions by peroxidase gene-based markers.
BACKGROUND
Peroxidase, a plant-specific oxidoreductase, is a heme-containing glycoprotein encoded by a large multigenic family in plants. Plant peroxidases (POXs, EC 1.11.1.7) play important roles in many self-defense interactions in plants. Here, 67 common bean (Phaseolus vulgaris L.) genotypes were studied using a POX gene-based marker method. Comparison of POX genes could resolve evolutionary relationships in common bean.


RESULTS
Eighty fragments were obtained with 20 primer pairs that amplified one (POX8c) to eight (ATP29) bands, with a mean of four bands per primer pair. The average (polymorphic information content) PIC value for the POX products was 0.40. The maximum variation (93%) was found between Turkey (#33) and India (#52) and between Antalya (#33) and India (#53). The minimum variation (0%) was found among four pairs: Bozdag (#2) and Karadeniz (#38), Kirklareli (#11) and Turkey (#15, 16, 43), Bandirma (#13) and Turkey (#15, 16, 43), and Kirklareli (#10) and Bandirma (#22). UPGMA was used to discriminate the common bean genotypes into five clusters, while STRUCTURE software was used to investigate the genetic population structure.


CONCLUSIONS
The results showed that POX gene family markers can be used to study genotypic diversity and provide new information for breeding programs and common bean improvement practices.
Department of Bioengineering, Ege University, 35100, Bornova-Izmir, Turkey.
Seda
S
Nemli
Hilal Betul
HB
Kaya
Bahattin
B
Tanyolac
D001947:Breeding; D018744:DNA, Plant / Q000032:analysis; D005787:Gene Frequency; D017343:Genes, Plant; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D007194:India; D009195:Peroxidase / Q000235:genetics*; D027805:Phaseolus / Q000201:enzymology* / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014421:Turkey
D018744:DNA, Plant; D005819:Genetic Markers; D009195:Peroxidase
common bean; genetic diversity; peroxidase gene-based markers; population structure