Chromosomal location by F1 monosomic analysis of endosperm proteins in bread wheat : 1. One-dimensional electrophoresis of glutenins and gliadins.
A new methodology to determine the chromosomal location and allelic differences of endosperm proteins in common wheat cultivars by analysis of monosomic intervarietal hybrids is reported. Endosperm proteins from the common wheats Chinese Spring, Capelle Desprez, Holdfast and Pane 247 are studied using monosomic F1 for the chromosomes of homoeologous groups 1 and 6. The proteins were fractionated by two electrophoretical techniques, SDS- and A-PAGE. The use of monosomic offers a remarkable advantage over the utilization of intervarietal substitution lines, because it permits a quicker characterization and earlier evaluation of basic material to be used in breeding programmes.
Department of Cellular Biology and Genetics, University of Alcalá de Henares. Campus Universitario, E-28871, Alcalá de Henares, Madrid, Spain.
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