Oxidative stress-related enzyme polymorphisms associated with the immunological biomarkers levels in heavy drinkers in Taiwan.
BACKGROUND
Excessive alcohol intake can result in the oxidative stress in cells and the genetic variations of alcohol-metabolizing enzymes are responsible for the different degrees of toxicity of alcohol in several organs, such as the liver and immunological systems. We hypothesized that the alteration of oxidative stress due to some genetic variations of oxidative stress-related enzymes could result in changes of specific biomarkers, and heavy drinkers could be cautioned about the predictive likelihood to induce drinking-induced diseases.


METHODS
A total of 108 heavy drinkers and 106 nonheavy drinkers were enrolled and the hematological, biochemical, and immunological tests were measured; the genotypes of oxidative stress-related enzymes, including manganese superoxide dismutase (MnSOD1183T>C), glutathione peroxidase 1 (GPX1Pro198Leu), catalase (CAT-262C>T), and myeloperoxidase (MPO-463G>A), were assayed by real-time polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (PCR-RFLP).


RESULTS
For the males, the levels of carbohydrate-deficient transferrin (CDT), malondialdehyde (MDA), CD4(+), immunoglobulin G (IgG), immunoglobulin M (IgM), and IL-6 were significantly different between the two groups. Furthermore, there were higher proportions of CD19(+) cells and lower TNF-α levels in heavy drinkers with the MnSOD C carriers, and there were higher percentages of CD19(+) cells and IL-6 levels in heavy drinkers with the combined genotypes of MnSOD C carriers and MPO A carriers.


CONCLUSIONS
Our findings indicate that heavy drinkers may be cautioned predictive likelihood for them to induce drinking-induced diseases by analyzing their MnSOD genotypes and immunological biomarkers.
Department of Pathology and Laboratory Medicine, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
Yang-Ming
YM
Tseng
Shih-Meng
SM
Tsai
Chun-Chin
CC
Lin
Yi-Ru
YR
Jin
Wei-Hao
WH
Yeh
Jen-Kuei
JK
Hsiao
Chi-Fen
CF
Chen
Wu-Hsiang
WH
Lan
Li-Yu
LY
Tsai
D000328:Adult; D000368:Aged; D000428:Alcohol Drinking / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology*; D015703:Antigens, CD / Q000097:blood*; D015415:Biomarkers; D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000235:genetics*; D010088:Oxidoreductases / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D013624:Taiwan; D055815:Young Adult
D015703:Antigens, CD; D015415:Biomarkers; D016207:Cytokines; D010088:Oxidoreductases
alcohol; heavy drinkers; manganese superoxide dismutase (MnSOD); oxidative stress-related enzymes; polymorphisms