Influence of the formulation on the maximum tolerated doses of brain targeted nanoparticles of gallic acid by oral administration in Wistar rats.
OBJECTIVE
The objective of the present investigation was to study the effect of pure gallic acid (GA) and its Tween 80 coated chitosan nanoparticles (cGANP) on the maximum tolerated dose (MTD) using Wistar rats.


METHODS
The animals were administered with increasing doses (dose increasing rate = 10%) of GA and cGANP orally for 28 consecutive days until the MTD was found. The animals were monitored for bodyweight, weight indices, behavioural, biochemical and histopathological changes.


RESULTS
The MTD was found to be 750 mg/kg for GA and was increased to 825 mg/kg for cGANP, which indicated a significantly greater tolerance of cGANP. Slight behavioural and biochemical changes were observed above the MTD. At the MTD no significant behavioural, biochemical, histopathological changes were observed.


CONCLUSIONS
The brain targeted nanoparticles displayed considerable improvement in the tolerance profile of the drug as compared with the free drug.
Department of Pharmaceutical Sciences, G. J. University of Science & Technology, Hisar, India.
Kalpana
K
Nagpal
Shailendra Kumar
SK
Singh
Dinanath
D
Mishra
D000284:Administration, Oral; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000187:drug effects*; D002626:Chemistry, Pharmaceutical; D005707:Gallic Acid / Q000008:administration & dosage*; D008297:Male; D020714:Maximum Tolerated Dose; D053758:Nanoparticles / Q000008:administration & dosage*; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
D005707:Gallic Acid
brain targeting; gallic acid; histopathology; maximum tolerated dose; nanoparticles