Callus formation from mesophyll protoplasts of Fagus sylvatica L.
Viable protoplasts of European beech (Fagus sylvatica L.) were isolated from sterilized young leaves of juvenile (3-5 years) and mature (40 years) trees. Isolation in a saline solution containing 0.5% (w/v) Pectinol and 2% (w/v) Cellulase R-10 yielded 3×10(7) protoplasts per gram fresh weight. Protoplast culture in modified Kao and Michayluk (1975) medium resulted in cell wall regeneration and sustained cell divisions with the formation of colonies and microcalli.
Botanisches Institut der Johann Wolfgang Goethe-Universität, Siesmayerstrasse 70, D-6000, Frankfurt am Main, Germany.
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Kohlenbach