Effect of near-infrared light exposure on mitochondrial signaling in C2C12 muscle cells.
Near-infrared (NIR) light is a complementary therapy used to treat musculoskeletal injuries but the underlying mechanisms are unclear. Acute NIR light treatment (~800-950 nm; 22.8 J/cm(2)) induced a dose-dependent increase in mitochondrial signaling (AMPK, p38 MAPK) in differentiated muscle cells. Repeated NIR light exposure (4 days) appeared to elevate oxidative stress and increase the upstream mitochondrial regulatory proteins AMPK (3.1-fold), p38 (2.8-fold), PGC-1α (19.7%), Sirt1 (26.8%), and reduced RIP140 (23.2%), but downstream mitochondrial regulation/content (Tfam, NRF-1, Sirt3, cytochrome c, ETC subunits) was unaltered. Our data indicates that NIR light alters mitochondrial biogenesis signaling and may represent a mechanistic link to the clinical benefits.
Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, FL 32611, United States.
Linda M-D
LM
Nguyen
Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, FL 32611, United States.
Angelina G
AG
Malamo
Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, FL 32611, United States.
Kelly A
KA
Larkin-Kaiser
Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, FL 32611, United States.
Paul A
PA
Borsa
Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, FL 32611, United States. Electronic address: padhihetty@hhp.ufl.edu.
Peter J
PJ
Adhihetty
D000818:Animals; D007259:Infrared Rays; D051379:Mice; D008928:Mitochondria / Q000502:physiology* / Q000528:radiation effects*; D063269:Mitochondrial Turnover / Q000528:radiation effects*; D032342:Muscle Cells / Q000502:physiology* / Q000528:radiation effects*; D015398:Signal Transduction
Mitochondrial biogenesis; NIR light; Oxidative stress; Photobiomodulation; Reactive oxygen species