Optimization of steady-state ¹³C-labeling experiments for metabolic flux analysis.
While steady-state (13)C metabolic flux analysis is a powerful method for deducing multiple fluxes in the central metabolic network of heterotrophic and mixotrophic plant tissues, it is also time-consuming and technically challenging. Key steps in the design and interpretation of steady-state (13)C labeling experiments are illustrated with a generic protocol based on applications to plant cell suspension cultures.
Department of Plant Sciences, University of Oxford, Oxford, UK.
Nicholas J
NJ
Kruger
Shyam K
SK
Masakapalli
R George
RG
Ratcliffe
D000465:Algorithms; D017360:Arabidopsis / Q000166:cytology / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002247:Carbon Isotopes / Q000378:metabolism; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D003198:Computer Simulation; D007700:Kinetics; D064688:Metabolic Flux Analysis; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D009010:Monte Carlo Method; D059828:Plant Cells / Q000378:metabolism; D013194:Staining and Labeling
D002247:Carbon Isotopes