Analysis of neutral lipids from microalgae by HPLC-ELSD and APCI-MS/MS.
A method was developed to analyze neutral lipids through the use of three triglycerides, four free fatty acids, six di- and four mono-glycerides standards by high performance liquid chromatography (HPLC) normal phase coupled with either with evaporative light scattering detector (ELSD) or with mass spectrometry (MS) operating in atmospheric pressure chemical ionization (APCI) mode. The method was applied to the determination of the neutral lipid fraction from a Botryococcus braunii race A (B. braunii) culture. This method led us to identify neutral lipids synthesized by B. braunii in a single analysis within 45min through HPLC-APCI-MS/MS technique.
UMR Qualisud (CIRAD, Université Montpellier II, UM1, Sup Agro), Place E. Bataillon, 34095 Montpellier Cedex 5, France; Total SA, PERL-Pôle d'Etudes et de Recherche de Lacq, BP 47, 64170 Lacq, France. Electronic address: florentin.donot@univ-montp2.fr.
F
F
Donot
G
G
Cazals
Z
Z
Gunata
D
D
Egron
J
J
Malinge
C
C
Strub
A
A
Fontana
S
S
Schorr-Galindo
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008055:Lipids / Q000032:analysis* / Q000737:chemistry*; D058086:Microalgae / Q000737:chemistry*; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*
D008055:Lipids
APCI-MS/MS; ELSD; HPLC; Microalgae; Neutral lipids; SPE