Study on human intestinal bacterium Blautia sp. AUH-JLD56 for the conversion of arctigenin to (-)-3'-desmethylarctigenin.
Arctium lappa L. (A. lappa) is a popularly used vegetable as well as herbal medicine. Human intestinal microflora was reported to convert arctiin, the lignan compound with highest content in the dried fruits of Arctium lappa, to a series of metabolites. However, the specific bacterium responsible for the formation of 3'-desmethylarctigenin (3'-DMAG), the most predominant metabolite of arctiin by rat or human intestinal microflora, has not been isolated yet. In the present study, we isolated one single bacterium, which we named Blautia sp. AUH-JLD56, capable of solely biotransforming arctiin or arctigenin to (-)-3'-DMAG. The structure of the metabolite 3'-DMAG was elucidated by electrospray ionization mass spectrometry (ESI-MS) and (1)H and (13)C nuclear magnetic resonance spectroscopy. The biotransforming kinetics and maximum biotransforming capacity of strain AUH-JLD56 was investigated. In addition, the metabolite 3'-DMAG showed significantly higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity than that of the substrate arctigenin at the concentrations tested.
College of Life Sciences, Agricultural University of Hebei , No. 289, Lingyusi Street, Baoding 071001, Hebei Province, China.
Ming-Yue
MY
Liu
Meng
M
Li
Xiu-Ling
XL
Wang
Peng
P
Liu
Qing-Hong
QH
Hao
Xiu-Mei
XM
Yu
D031170:Arctium / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D001711:Biotransformation; D005638:Fruit / Q000378:metabolism; D005663:Furans / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000737:chemistry / Q000382:microbiology*; D007700:Kinetics; D017705:Lignans / Q000737:chemistry* / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry* / Q000378:metabolism*
D005663:Furans; D017705:Lignans; D010936:Plant Extracts; C071942:arctigenin