Expression of carbonic anhydrase, cystic fibrosis transmembrane regulator (CFTR) and V-H(+)-ATPase in the lancelet Branchiostoma lanceolatum (Pallas, 1774).
Sequencing of the amphioxus genome revealed that it contains a basic set of chordate genes involved in development and cell signaling. Despite the availability of genomic data, up till now no studies have been addressed on the comprehension of the amphioxus osmoregulation. Using primers designed on Branchiostoma floridae carbonic anhydrase (CA) II, cystic fibrosis transmembrane regulator (CFTR) and V-H(+)-ATPase, a 100bp long region, containing the protein region recognized by the respective antibodies, has been amplified and sequenced in B. lanceolatum indicating the presence of hortologous V-ATPase, CFTR and carbonic anhydrase II genes in Branchiostoma lanceolatum. Immunohistochemical results showed that all three transporting proteins are expressed in almost 90% of epithelial cells of the skin in B. lanceolatum adults with a different degree of positivity in different regions of body wall and with a different localization in the cells. The comparison of results between young and adult lancelets showed that the distribution of these transporters is quite different. Indeed, in the young specimens the expression pattern of all tested molecules appears concentrated at the gut level, whereas in adult the gut loses its key role that is mostly supported by skin.
Department of Life Sciences, University of Modena and Reggio Emilia, via Campi, 213/D, I-41125 Modena, Italy. Electronic address: aurora.pederzoli@unimore.it.
Aurora
A
Pederzoli
Department of Life Sciences, University of Modena and Reggio Emilia, via Campi, 213/D, I-41125 Modena, Italy.
Mauro
M
Mandrioli
Department of Life Sciences, University of Modena and Reggio Emilia, via Campi, 213/D, I-41125 Modena, Italy.
Lucrezia
L
Mola
D000136:Acid-Base Equilibrium; D000818:Animals; D002256:Carbonic Anhydrases / Q000378:metabolism*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000378:metabolism*; D000078404:Epidermal Cells; D004817:Epidermis / Q000201:enzymology; D007413:Intestinal Mucosa / Q000166:cytology / Q000201:enzymology; D064175:Lancelets / Q000166:cytology / Q000201:enzymology*; D025262:Vacuolar Proton-Translocating ATPases / Q000378:metabolism*
D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D025262:Vacuolar Proton-Translocating ATPases; D002256:Carbonic Anhydrases
Amphioxus; Development; Osmoregulation; Skin; pH regulation