Simultaneous determination of allantoin and glycolic acid in snail mucus and cosmetic creams with high performance liquid chromatography and ultraviolet detection.
A new methodology for simultaneous quantitative analysis of allantoin and glycolic acid in snail mucus and cosmetic creams was developed. HPLC separation was achieved a Synergi-Hydro RP column within 7min using isocratic elution with potassium phosphate (pH 2.7; 10mM) at a flow rate of 0.7mL/min at 30°C. Sample pretreatment was performed by dilution of mucus or cosmetic cream in the elution buffer, heating at 60°C for 20min, adjusting the pH to 2.9 and purification with hexane extraction. Linearity was determined with spiked samples and the LLOQ values of 0.0125 and 0.2500mg/mL were determined for allantoin and glycolic acid, respectively. Accuracy and intra- and inter-day repeatability were studied at three levels of concentrations (0.04, 0.08 and 0.16mg/mL for allantoin and 0.1, 1.5 and 4.0mg/mL for glycolic acid) using spiked mucus and cream base samples; mean values of recovery were in the range of 96.81-102.42% in all matrices tested, whereas the respective RSDs (%Relative Standard Deviation) were less than 3.04% in all cases. Spiked mucus and cream samples were stable (RSD<4.16 and relative error<4.34%) at room temperature and at 4°C for 1 week and at -18°C for 6 months; samples were also stable after three freeze-thaw cycles. The method was applied to the analysis of different lots of snail mucus, and of three commercial creams containing snail mucus.
Department of Pharmacy, University of Patras, , 26504 Patras, Greece.
Mohamed Ahmed S
MA
El Mubarak
Fotini N
FN
Lamari
Christos
C
Kontoyannis
D000481:Allantoin / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003358:Cosmetics / Q000737:chemistry*; D006016:Glycolates / Q000032:analysis*; D009093:Mucus / Q000737:chemistry*; D015203:Reproducibility of Results; D063465:Skin Cream / Q000737:chemistry*; D012908:Snails / Q000737:chemistry*; D014466:Ultraviolet Rays
D003358:Cosmetics; D006016:Glycolates; C031149:glycolic acid; D000481:Allantoin
Allantoin; Cosmetic cream; Glycolic acid; HPLC; Snail mucus; Validation