Alumina-zirconia composites functionalized with laminin-1 and laminin-5 for dentistry: effect of protein adsorption on cellular response.
The present paper describes a study on laminin interaction with the surface of two alumina-zirconia composites with different percentages of ZrO2, both with submicrometric grain size. As major molecules within the basement membrane (BM), laminins are important protein fragments for epithelial cell adhesion and migration. On the other hand, alumina-zirconia composites are very attractive materials for dental applications due to their esthetic and mechanical properties. X-Ray photoelectron spectroscopy and atomic force microscopy were used to study the adsorption of two types of laminin, laminin-1 (Ln-1) and laminin-5 (Ln-5), onto the ceramics surfaces. The in vitro cell response was determined by intracellular phosphorylation of major kinases. Ceramics samples functionalized with laminins showed better cellular activation than untreated specimens; furthermore, cellular activation was found to be greater for the composite with higher percentage in zirconia when functionalized with Ln-5, whereas the adsorption of Ln-1 resulted in a greater activation for the alumina-rich oxide.
Chemistry Department and NIS Excellence Centre, Università degli Studi di Torino, Via P. Giuria 7, 10125 Turin, Italy. Electronic address: anne.vallee@uvsq.fr.
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Vallée
CNR-IMAMOTER, Strada delle Cacce 73, 10135 Turin, Italy.
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Faga
Department of Surgery, Dental School, Università degli Studi di Torino, Via Nizza 230, 10126 Turin, Italy.
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Mussano
Chemistry Department and NIS Excellence Centre, Università degli Studi di Torino, Via P. Giuria 7, 10125 Turin, Italy.
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Catalano
Molecular Biotechnology Center, Università degli Studi di Torino, Via Nizza 52, 10126 Turin, Italy.
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Tolosano
Department of Surgery, Dental School, Università degli Studi di Torino, Via Nizza 230, 10126 Turin, Italy.
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Carossa
Molecular Biotechnology Center, Università degli Studi di Torino, Via Nizza 52, 10126 Turin, Italy.
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Altruda
Chemistry Department and NIS Excellence Centre, Università degli Studi di Torino, Via P. Giuria 7, 10125 Turin, Italy.
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Martra
D000327:Adsorption / Q000187:drug effects; D000537:Aluminum Oxide / Q000737:chemistry*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000494:pharmacology*; D002477:Cells / Q000166:cytology* / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D003813:Dentistry; D006367:HeLa Cells; D006801:Humans; D007797:Laminin / Q000494:pharmacology*; D051379:Mice; D018625:Microscopy, Atomic Force; D010766:Phosphorylation / Q000187:drug effects; D056951:Photoelectron Spectroscopy; D013499:Surface Properties; D015040:Zirconium / Q000737:chemistry*; D000097794:Kalinin
D000537:Aluminum Oxide; D015815:Cell Adhesion Molecules; D016207:Cytokines; D007797:Laminin; D015040:Zirconium; D000097794:Kalinin; C121346:laminin 1; C028541:zirconium oxide
Alumina–zirconia composites; Atomic force microscopy; Cell response; Laminins 5 and 1; X ray photoelectron spectroscopy