Determination of phenol and catechol concentrations with oxygen probes coated with immobilized enzymes or immobilized cells.
The enzyme phenol 2-hydroxylase was immobilized on Sepharose and used in conjunction with an O2 electrode for quantitating phenol. Similarly, catechol 1,2-oxygenase was used for quantitating catechol. A third probe was prepared by immobilization ofTrichosporon cutaneum cells rather than purified phenol 2-hydroxylase for phenol quantitation. The whole cell system gave results comparable to the immobilized enzyme system.
Department of Biochemistry and Biotechnology, The Royal Institute of Technology, S-100 44, Stockholm, Sweden.
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