Rapid kill-novel endodontic sealer and Enterococcus faecalis.
With growing concern over bacterial resistance, the identification of new antimicrobial means is paramount. In the oral cavity microorganisms are essential to the development of periradicular diseases and are the major causative factors associated with endodontic treatment failure. As quaternary ammonium compounds have the ability to kill a wide array of bacteria through electrostatic interactions with multiple anionic targets on the bacterial surface, it is likely that they can overcome bacterial resistance. Melding these ideas, we investigated the potency of a novel endodontic sealer in limiting Enterococcus faecalis growth. We used a polyethyleneimine scaffold to synthesize nano-sized particles, optimized for incorporation into an epoxy-based endodontic sealer. The novel endodontic sealer was tested for its antimicrobial efficacy and evaluated for biocompatibility and physical eligibility. Our results show that the novel sealer foundation affixes the nanoparticles, achieving surface bactericidal properties, but at the same time impeding nanoparticle penetration into eukaryotic cells and thereby mitigating a possible toxic effect. Moreover, adequate physical properties are maintained. The nanosized quaternary amine particles interact within minutes with bacteria, triggering cell death across wide pH values. Throughout this study we demonstrate a new antibacterial perspective for endodontic sealers; a novel antibacterial, effective and safe antimicrobial means.
Department of Prosthodontics, Faculty of Dentistry, The Hebrew University-Hadassah, Jerusalem, Israel.
Nurit
N
Beyth
Dana
D
Kesler Shvero
Nathan
N
Zaltsman
Yael
Y
Houri-Haddad
Itzhak
I
Abramovitz
Michael Perez
MP
Davidi
Ervin I
EI
Weiss
D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003764:Dental Materials / Q000138:chemical synthesis / Q000494:pharmacology*; D004708:Endodontics; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000254:growth & development; D004853:Epoxy Resins / Q000737:chemistry / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D050296:Microbial Viability / Q000187:drug effects; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011094:Polyethyleneimine / Q000737:chemistry*; D011671:Pulpitis / Q000517:prevention & control
D000900:Anti-Bacterial Agents; D003764:Dental Materials; D004853:Epoxy Resins; D011094:Polyethyleneimine