Transformation of Brassica oleracea with an S-locus gene from B. campestris changes the self-incompatibility phenotype.
An SLG gene derived from the S-locus and encoding and S-locus-specific glycoprotein of Brassica campestris L. was introduced via Agrobacterium-mediated transformation into B. oleracea L. A self-incompatible hybrid and another with partial self-compatibility were used as recipients. The transgenic plants were altered in their pollen-stigma interaction and were fully compatible upon self-pollination. Reciprocal crosses between the transgenic plants and untransformed control plants indicated that the stigma reaction was changed in one recipient strain while the pollen reaction was altered in the other. Due to interspecific incompatibility, we could not demonstrate whether or not the introduced SLG gene confers a new allelic specificity in the transgenic plants. Our results show that the introduced SLG gene perturbs the self-incompatibility phenotype of stigma and pollen.
Section of Plant Biology, Division of Biological Sciences, Cornell University, 14853, Ithaca, NY, USA.
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K
Toriyama
J C
JC
Stein
M E
ME
Nasrallah
J B
JB
Nasrallah