title: Aromadendrin Inhibits Lipopolysaccharide-Induced Nuclear Translocation of NF-κB and Phosphorylation of JNK in RAW 264.7 Macrophage Cells.
abstract: Aromadendrin, a flavonol, has been reported to possess a variety of pharmacological activities such as anti-inflammatory, antioxidant, and anti-diabetic properties. However, the underlying mechanism by which aromadendrin exerts its biological activity has not been extensively demonstrated. The objective of this study is to elucidate the anti-inflammatory mechanism of aromadedrin in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. Aromadendrin significantly suppressed LPS-induced excessive production of pro-inflammatory mediators such as nitric oxide (NO) and PGE2. In accordance, aromadendrin attenuated LPSinduced overexpression iNOS and COX-2. In addition, aromadendrin significantly suppressed LPS-induced degradation of IκB, which sequesters NF-κB in cytoplasm, consequently inhibiting the nuclear translocation of pro-inflammatory transcription factor NF- κB. To elucidate the underlying signaling mechanism of anti-inflammatory activity of aromadendrin, MAPK signaling pathway was examined. Aromadendrin significantly attenuated LPS-induced activation of JNK, but not ERK and p38, in a concentration-dependent manner. Taken together, the present study clearly demonstrates that aromadendrin exhibits anti-inflammatory activity through the suppression of nuclear translocation of NF-κB and phosphorylation of JNK in LPS-stimulated RAW 264.7 macrophage cells.
authors.affiliation: Department of Pharmacology, College of Medicine.
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Nam Ho
authors.initials: NH
authors.lastname: Kim
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Jun-Ho
authors.initials: JH
authors.lastname: Park
authors.forename: Seung-Yeon
authors.initials: SY
authors.lastname: Shin
authors.forename: Yong-Soo
authors.initials: YS
authors.lastname: Kwon
authors.forename: Hee Jae
authors.initials: HJ
authors.lastname: Lee
authors.forename: Sung-Soo
authors.initials: SS
authors.lastname: Kim
authors.forename: Wanjoo
authors.initials: W
authors.lastname: Chun
keywords: Aromadendrin; COX-2; JNK; Lipopolysaccharide; NF-κB; RAW 264.7 cells; iNOS