title: Effects of bio-Au nanoparticles on electrochemical activity of Shewanella oneidensis wild type and ΔomcA/mtrC mutant.
abstract: Both Shewanella oneidensis MR-1 wild type and its mutant ΔomcA/mtrC are capable of transforming Au(III) into Au nanoparticles (AuNPs). Cyclic voltammetry reveals a decrease in redox current after the wild type is exposed to Au(III) but an increase in oxidation current for the mutant. The peak current of the wild type is much higher than that of the mutant before the exposure of Au(III), but lower than that of the mutant after the formation of AuNPs. This suggests that damage to the electron transfer chain in the mutant could be repaired by AuNPs to a certain extent. Spectroscopy and SDS-PAGE analysis indicate a decrease in cell protein content after the formation of AuNPs, which provides a convenient way to detect intracellular information on cells.
authors.affiliation: Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen, Fujian Province, 361021, China.
authors.forename: Ranran
authors.initials: R
authors.lastname: Wu
authors.forename: Li
authors.initials: L
authors.lastname: Cui
authors.forename: Lixiang
authors.initials: L
authors.lastname: Chen
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.forename: Changli
authors.initials: C
authors.lastname: Cao
authors.forename: Guoping
authors.initials: G
authors.lastname: Sheng
authors.forename: Hanqing
authors.initials: H
authors.lastname: Yu
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003574:Cytochrome c Group / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D055664:Electrochemical Techniques; D004566:Electrodes; D004591:Electrophoresis, Polyacrylamide Gel; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D009154:Mutation; D010084:Oxidation-Reduction; D020592:Shewanella / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D013059:Spectrum Analysis, Raman
chemical_list: D001425:Bacterial Outer Membrane Proteins; D003574:Cytochrome c Group; D006046:Gold; C433527:MtrC protein, Shewanella