title: Molecular chaperone function of Arabidopsis thaliana phloem protein 2-A1, encodes a protein similar to phloem lectin.
abstract: Although several phloem sap proteins have been identified from protein extracts of heat-treated Arabidopsis seedlings using FPLC gel filtration columns, many of the physiological roles played by these proteins remain to be elucidated. We functionally characterized a phloem protein 2-A1, which encodes a protein similar to phloem lectin. Using a bacterially expressed recombinant protein of AtPP2-A1, we found that it performs dual functions, showing both molecular chaperone activity and antifungal activity. mRNA expression of the AtPP2-1 gene was induced by diverse external stresses such as pathogens, and other signaling molecules, such as ethylene. These results suggest that the AtPP2-A1 molecular chaperone protein plays a critical role in the Arabidopsis defense system against diverse external stresses including fungal pathogenic attack and heat shock.
authors.affiliation: Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843, USA; Division of Applied Life Sciences and PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea. Electronic address: jrlee73@tamu.edu.
authors.forename: Jung Ro
authors.initials: JR
authors.lastname: Lee
authors.affiliation: Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843, USA.
authors.forename: Kara A
authors.initials: KA
authors.lastname: Boltz
authors.affiliation: Division of Applied Life Sciences and PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea. Electronic address: sylee@gnu.ac.kr.
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D005658:Fungi; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D037121:Plant Lectins / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D029681:Arabidopsis Proteins; D018832:Molecular Chaperones; C552547:PP2-A1 protein, Arabidopsis; D037121:Plant Lectins; C091028:phloem lectin
keywords: Antifungal activity; Arabidopsis thaliana; Lectin; Molecular chaperone; Phloem protein