title: IL-1 alpha regulates CXCL1, CXCL10 and ICAM1 in network form in oral keratinocytes.
abstract: BACKGROUND
  Keratinocytes are the major constituent of epithelial cells at skin and mucosal surfaces such as oral mucosa. In our previous study, it was indicated that oral keratinocytes play active roles in oral mucosal adaptive immune response and an IL-alpha sub-network was identified. In the past decades IL-1, a revolutionarily ancient cytokine, has attracted our attention for its important roles in innate and adaptive immune responses. In this study we aim to show the IL-alpha sub-network in oral keratinocytes and explore its relevance in oral lichen planus (OLP).
  
  
  METHODS
  We first tested whether IL-1alpha regulated its sub-network genes including CXCL1, CXCL10, and ICAM1 mRNA levels in time and concentration dependent manners by real time PCR. Then we investigated the expression of IL-1alpha and CXCL1 in OLP tissues by immunohistochemical staining.
  
  
  RESULTS
  IL-1alpha regulated its sub-network genes including CXCL1, CXCL10, and ICAM1 mRNA levels in time dependent but not in concentration dependent manner. Immunohistochemistry studies showed that IL-1alpha and CXCL1 were expressed in OLP tissues, which were only detected in tissue transudate and whole unstimulated saliva in previous studies.
  
  
  CONCLUSIONS
  IL-1alpha regulates CXCL1, CXCL10, and ICAM1 in network form in oral keratinocytes. A complete characterization of the IL-1alpha sub-network will shed light on the exploration of IL-1 as the therapeutic target in OLP and help to illuminate the multiple regulatory functions of keratinocytes in oral mucosa or even in other mucosa sites.
authors.affiliation: Department of Oral Medicine, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Wu
authors.forename: Rui
authors.initials: R
authors.lastname: Du
authors.forename: Yun
authors.initials: Y
authors.lastname: Hong
authors.forename: Lihua
authors.initials: L
authors.lastname: Jia
authors.forename: Qi
authors.initials: Q
authors.lastname: Zeng
authors.forename: Bin
authors.initials: B
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D054360:Chemokine CXCL1 / Q000235:genetics*; D054357:Chemokine CXCL10 / Q000235:genetics*; D017931:DNA Primers; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics*; D053582:Interleukin-1alpha / Q000502:physiology*; D015603:Keratinocytes / Q000378:metabolism*; D017676:Lichen Planus, Oral / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009055:Mouth / Q000166:cytology / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D054360:Chemokine CXCL1; D054357:Chemokine CXCL10; D017931:DNA Primers; C573420:ICAM1 protein, human; D053582:Interleukin-1alpha; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1