title: Genetic differentiation and efficient sex-specific marker development of a pair of Y- and X-linked markers in yellow catfish.
abstract: Pf62-Y and Pf62-X is a pair of allelic Y chromosome-linked and X chromosome-linked markers, and have been used to identify YY super-males, XY males and XX females for commercial production of all-male populations in yellow catfish (Pelteobagrus fulvidraco). However, the SCAR primers used previously have only two nucleotide difference, which restricts the wide utility because of nucleotide polymorphism. In this study, a continuous 8102 bp Pf62-Y sequence and a 5362 bp Pf62-X sequence have been cloned by genome walking, and significant genetic differentiation has been revealed between the corresponding X and Y chromosome allele sequences. Moreover, three pairs of primers were designed to efficiently identify YY super-males, XY males and XX females in an artificial breeding population, and to distinguish XY males and XX females in various wild populations. Together, the three new sex-specific genetic markers develop a highly stable and efficient method for genetic sex identification and sex control application in sustainable aquaculture of all-male yellow catfish.
authors.affiliation: 1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate University of the Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Dan
authors.forename: Jie
authors.initials: J
authors.lastname: Mei
authors.forename: Da
authors.initials: D
authors.lastname: Wang
authors.forename: Jian-Fang
authors.initials: JF
authors.lastname: Gui
mesh_terms: D000818:Animals; D002397:Catfishes / Q000235:genetics*; D016386:Chromosome Walking; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D008297:Male; D012737:Sex Factors; D014960:X Chromosome; D014998:Y Chromosome
chemical_list: D005819:Genetic Markers
keywords: All-male fish.; Sex-specific marker; YY super-males; Yellow catfish; genome walking