title: Detection of genetically modified maize in processed foods sold commercially in iran by qualitative PCR.
abstract: Detection of genetically modified organisms (GMOs) in food is an important issue for all the subjects involved in food control and customer's right. Due to the increasing number of GMOs imported to Iran during the past few years, it has become necessary to screen the products in order to determine the identity of the consumed daily foodstuffs. In this study, following the extraction of genomic DNA from processed foods sold commercially in Iran, qualitative PCR was performed to detect genetically modified maize. The recombinant DNA target sequences were detected with primers highly specific for each investigated transgene such as CaMV35s gene, Bt-11, MON810 and Bt-176 separately. Based on the gel electrophoresis results, Bt- 11 and MON810 events were detected in some maize samples, while, in none of them Bt- 176 modified gene was detected. For the first time, the results demonstrate the presence of genetically modified maize in Iranian food products, reinforcing the need for the development of labeling system and valid quantitative methods in routine analyses.
authors.affiliation: Food and Drug Laboratory Research Center, Food and Drug Organization, Ministry of Health and Medical Education, Tehran, Iran. ; Department of Molecular Biology, Food and Drug Control Reference Laboratory, Ministry of Health and Medical Education, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Rabiei
authors.forename: Mehrangiz
authors.initials: M
authors.lastname: Mehdizadeh
authors.forename: Hossein
authors.initials: H
authors.lastname: Rastegar
authors.forename: Hossein
authors.initials: H
authors.lastname: Vahidi
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Alebouyeh
keywords: DNA extraction; Detection; GMO; Maize; Polymerase chain reaction.