title: Neutrophils suppress γδ T-cell function.
abstract: γδ T cells have been shown to stimulate the recruitment and activation of neutrophils through the release of a range of cytokines and chemokines. Here, we investigated the reverse relationship, showing that human neutrophils suppress the function of human blood γδ T cells. We show that the upregulation of CD25 and CD69 expression, the production of IFN-γ, and the proliferation of γδ T cells induced by (E)-1-hydroxy-2-methylbut-2-enyl 4-diphosphate are inhibited by neutrophils. Spontaneous activation of γδ T cells in culture is also suppressed by neutrophils. We show that inhibitors of prostaglandin E2 and arginase I do not exert any effect, although, in contrast, catalase prevents the suppression of γδ T cells induced by neutrophils, suggesting the participation of neutrophil-derived ROS. We also show that the ROS-generating system xanthine/xanthine oxidase suppresses γδ T cells in a similar fashion to neutrophils, while neutrophils from chronic granulomatous disease patients only weakly inhibit γδ T cells. Our results reveal a bi-directional cross-talk between γδ T cells and neutrophils: while γδ T cells promote the recruitment and the activation of neutrophils to fight invading pathogens, neutrophils in turn suppress the activation of γδ T cells to contribute to the resolution of inflammation.
authors.affiliation: Instituto de Medicina Experimental (IMEX) CONICET - Academia Nacional de Medicina, Buenos Aires, Argentina.
authors.forename: Florencia
authors.initials: F
authors.lastname: Sabbione
authors.forename: María L
authors.initials: ML
authors.lastname: Gabelloni
authors.forename: Glenda
authors.initials: G
authors.lastname: Ernst
authors.forename: María S
authors.initials: MS
authors.lastname: Gori
authors.forename: Gabriela
authors.initials: G
authors.lastname: Salamone
authors.forename: Matías
authors.initials: M
authors.lastname: Oleastro
authors.forename: Analía
authors.initials: A
authors.lastname: Trevani
authors.forename: Jorge
authors.initials: J
authors.lastname: Geffner
authors.forename: Carolina C
authors.initials: CC
authors.lastname: Jancic
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D016692:Receptors, Antigen, T-Cell, gamma-delta
keywords: Neutrophils; ROS; γδ T cells