title: Photo-cured PMMA/PEI core/shell nanoparticles surface-modified with Gd-DTPA for T1 MR imaging.
abstract: Herein, we introduced amine-functionalized core-shell nanoparticles (Polymethyl methacrylate/Polyethyleneimine; PMMA/PEI) with surface primary amines (3.15×10(5) groups/particle) and uniform size distribution (150-200nm) that were prepared by one-step photo-induced emulsion polymerization. Further PEI-surface was modified with diethylenetriamine pentaacetic acid (DTPA) and introduced with Gd(III). The modified particles possessing DTPA can entrap a high content of Gd(III) ions of over 5.5×10(4)Gd/particle with stable chelation (no release of free Gd) at least 7h. The Gd-DTPA-conjugated core-shell nanoparticles (PMMA/PEI-DTPA-Gd NPs) enhanced the MRI intensity more than Primovist (a commercial hepatic contrast agent). Moreover, the PMMA/PEI-DTPA-Gd NPs showed non-cytotoxicity up to 250μM in normal liver cells. Thus, in vitro data suggested the PMMA/PEI-DTPA-Gd NPs is promising delivery system as a superior MRI contrast agent, especially for hepatic lesion targeted MR imaging.
authors.affiliation: Utah-Inha DDS and Advanced Therapeutics, B-403 Meet-You-All Tower, Songdo Technopark, 7-50, Songdo-dong, Yeonsu-gu, Incheon 406-840, Republic of Korea; Department of Chemistry, Faculty of Science, Mahidol University, Nakhon Pathom 73170, Thailand.
authors.forename: Montri
authors.initials: M
authors.lastname: Ratanajanchai
authors.forename: Don Haeng
authors.initials: DH
authors.lastname: Lee
authors.forename: Panya
authors.initials: P
authors.lastname: Sunintaboon
authors.forename: Su-Geun
authors.initials: SG
authors.lastname: Yang
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003287:Contrast Media / Q000138:chemical synthesis* / Q000494:pharmacology / Q000528:radiation effects; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000494:pharmacology / Q000528:radiation effects; D004655:Emulsions; D019786:Gadolinium DTPA / Q000494:pharmacology; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008027:Light; D008099:Liver; D008279:Magnetic Resonance Imaging / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D004369:Pentetic Acid / Q000737:chemistry; D019047:Phantoms, Imaging; D055668:Photochemical Processes; D011094:Polyethyleneimine / Q000737:chemistry*; D058105:Polymerization; D019904:Polymethyl Methacrylate / Q000737:chemistry*
chemical_list: D003287:Contrast Media; D056831:Coordination Complexes; D004655:Emulsions; C073590:gadolinium ethoxybenzyl DTPA; D004369:Pentetic Acid; D011094:Polyethyleneimine; D019904:Polymethyl Methacrylate; D019786:Gadolinium DTPA
keywords: Core/shell nanoparticles; Gd(III); Gd–DTPA; Liver cancer; MRI; Photo-cured nanoparticles; Polyethyleneimine (PEI); Polymethyl methacrylate (PMMA); T1 contrast