title: The expression of PTEN in the development of mouse cochlear lateral wall.
abstract: Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is a tumor suppressor gene that regulates various cell processes including proliferation, growth, synaptogenesis, neural and glioma stem/progenitor cell renewal. In addition, PTEN can regulate sensory cell proliferation and differentiation of hair bundles in the mammalian cochlea. In this study we use immunofluorescence, Western blot and reverse transcriptase-polymerase chain reaction (RT-PCR) to reveal the expression of PTEN in the developing cochlear lateral wall, which is crucial for regulating K(+) homeostasis. Relatively high levels of PTEN are initially expressed in the marginal cells (MCs) of the lateral wall at embryonic day (E) 17.5 when they start to differentiate. Similarly high levels are subsequently expressed in differentiating root cells (RCs) at postnatal day (P) 3 and then in spiral ligament fibrocytes (SLFs) at P 10. In the mature cochlea, PTEN expression is low or undetectable in MCs and SLFs but it remains high in RCs and their processes. The expression pattern for PTEN in the developing lateral wall suggests that it plays a critical role in the differentiation of the cellular pathways that regulate K(+) homeostasis in the cochlea.
authors.affiliation: Department of Cell Physiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan. Electronic address: dongyy@med.kagawa-u.ac.jp.
authors.forename: Y
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Department of Cell Physiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan.
authors.forename: L
authors.initials: L
authors.lastname: Sui
authors.affiliation: Department of Cell Physiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Yamaguchi
authors.affiliation: Department of Cell Physiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kamitori
authors.affiliation: Department of Cell Physiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hirata
authors.affiliation: Department of Cell Physiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Hossain
authors.affiliation: Department of Cell Physiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Noguchi
authors.affiliation: Department of Cell Physiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Katagi
authors.affiliation: Division of Cancer Genetics, Medical Institute of Bioregulation, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nishio
authors.affiliation: Division of Cancer Genetics, Medical Institute of Bioregulation, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Suzuki
authors.affiliation: Department of Bioengineering, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, North Renmin Road 2999, Shanghai 201620, China.
authors.forename: X
authors.initials: X
authors.lastname: Lou
authors.affiliation: Department of Cell Physiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tokuda
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation; D003051:Cochlea / Q000254:growth & development* / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D051379:Mice; D008813:Mice, Inbred ICR; D051059:PTEN Phosphohydrolase / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055493:Spiral Ligament of Cochlea / Q000254:growth & development / Q000378:metabolism
chemical_list: D051059:PTEN Phosphohydrolase; C487757:Pten protein, mouse
keywords: 4,6-diamino-2-phenylindole; BC; BSA; DAPI; E; EDTA; EP; HC; IC; L19; MC; OHC; P; PBS; PCR; PI3 K; PIP(3); PTEN; RC; RT-PCR; SL; SLF; SM; SV; basal cell; bovine serum albumin; cochlea; embryonic day; endocochlear potential; ethylenediaminetetraacetic acid; fibrocytes; hair cell; intermediate cell; marginal cell; marginal cells; outer hair cell; phosphatase and tensin homolog deleted on chromosome 10; phosphate-buffered saline; phosphatidylinositol-3 kinase; phosphatidylinositol-3,4,5-triphosphate; polymerase chain reaction; postnatal day; reverse transcriptase PCR; ribosomal gene; root cell; root cells; scala media; spiral ligament; spiral ligament fibrocyte; stria vascularis