title: Integrins and their ligands are expressed in non-small cell lung cancer but not correlated with parameters of disease progression.
abstract: The aim of this study was to investigate the expression of integrins, their ligands, and integrin signaling-related molecules in a cohort of human primary non-small cell lung cancers (NSCLC). Formalin-fixed and paraffin-embedded tissue samples from 215 NSCLC were immunohistochemically stained using antibodies directed against αvβ3, αvβ5, αvβ6, αvβ8, αv, osteopontin, fibronectin, vitronectin, epidermal growth factor (EGFR), vascular endothelial growth factor receptor (VEGFR), and Ki67. Immunostaining of tumor, stroma, and endothelial cells was evaluated separately by quantity and intensity (tumor cells) or intensity (stroma and endothelial cells) expressed in an immunoreactivity score. We studied correlations between the staining patterns of the different markers and of marker expression with clinicopathological data and patient survival. In the majority of NSCLC, each marker was expressed in at least one tumor component. As expected, αv and αv integrin heterodimers were significantly co-expressed, as were integrins and EGFR. Vitronectin was expressed significantly more often in smaller (T-category) and in well-differentiated tumors; Ki67 index was higher in larger (T-category) and in poorly differentiated tumors. No significant correlation was found between any marker expression and gender, venous invasion, lymph vessel invasion, lymph node metastasis, or survival. Although integrin expression does not seem to be associated with indicators of progression of NSCLC, the expression of αvβ3 in 89 % and αvβ5 in 100 % of NSCLC is novel and merits to be further investigated.
authors.affiliation: Department of Pathology, Christian-Albrechts-University, Arnold-Heller-Str. 3, Haus 14, 24105, Kiel, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Böger
authors.forename: Holger
authors.initials: H
authors.lastname: Kalthoff
authors.forename: Simon L
authors.initials: SL
authors.lastname: Goodman
authors.forename: Hans-Michael
authors.initials: HM
authors.lastname: Behrens
authors.forename: Christoph
authors.initials: C
authors.lastname: Röcken
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry* / Q000401:mortality / Q000473:pathology; D004268:DNA-Binding Proteins / Q000032:analysis; D018450:Disease Progression; D066246:ErbB Receptors / Q000032:analysis / Q000037:antagonists & inhibitors; D006801:Humans; D007150:Immunohistochemistry; D016023:Integrins / Q000032:analysis*; D019394:Ki-67 Antigen / Q000032:analysis; D008024:Ligands; D008175:Lung Neoplasms / Q000737:chemistry* / Q000401:mortality / Q000473:pathology; D046888:Tissue Array Analysis; D014157:Transcription Factors
chemical_list: D004268:DNA-Binding Proteins; D016023:Integrins; D019394:Ki-67 Antigen; D008024:Ligands; C504003:TTF1 protein, human; D014157:Transcription Factors; D066246:ErbB Receptors