title: Inositol polyphosphate multikinase is a coactivator for serum response factor-dependent induction of immediate early genes.
abstract: Inositol polyphosphate multikinase (IPMK) is a notably pleiotropic protein. It displays both inositol phosphate kinase and phosphatidylinositol kinase catalytic activities. Noncatalytically, IPMK stabilizes the mammalian target of rapamycin complex 1 and acts as a transcriptional coactivator for CREB-binding protein/E1A binding protein p300 and tumor suppressor protein p53. Serum response factor (SRF) is a major transcription factor for a wide range of immediate early genes. We report that IPMK, in a noncatalytic role, is a transcriptional coactivator for SRF mediating the transcription of immediate early genes. Stimulation by serum of many immediate early genes is greatly reduced by IPMK deletion. IPMK stimulates expression of these genes, an influence also displayed by catalytically inactive IPMK. IPMK acts by binding directly to SRF and thereby enhancing interactions of SRF with the serum response element of diverse genes.
authors.affiliation: Department of Biological Sciences and KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.
authors.forename: Eunha
authors.initials: E
authors.lastname: Kim
authors.forename: Richa
authors.initials: R
authors.lastname: Tyagi
authors.forename: Joo-Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Jina
authors.initials: J
authors.lastname: Park
authors.forename: Young-Ran
authors.initials: YR
authors.lastname: Kim
authors.forename: Jiyoon
authors.initials: J
authors.lastname: Beon
authors.forename: Po Yu
authors.initials: PY
authors.lastname: Chen
authors.forename: Jiyoung Y
authors.initials: JY
authors.lastname: Cha
authors.forename: Solomon H
authors.initials: SH
authors.lastname: Snyder
authors.forename: Seyun
authors.initials: S
authors.lastname: Kim
mesh_terms: D000818:Animals; D047369:Chromatin Immunoprecipitation; D017931:DNA Primers / Q000235:genetics; D050881:E1A-Associated p300 Protein / Q000378:metabolism; D017781:Genes, Immediate-Early / Q000235:genetics / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D015151:Immunoblotting; D051379:Mice; D018345:Mice, Knockout; D046228:Microarray Analysis; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D026362:Serum Response Factor / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D015533:Transcriptional Activation / Q000235:genetics / Q000502:physiology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D017931:DNA Primers; D026362:Serum Response Factor; D016159:Tumor Suppressor Protein p53; D050881:E1A-Associated p300 Protein; C494361:Ep300 protein, mouse; D017853:Phosphotransferases (Alcohol Group Acceptor); C434257:inositol polyphosphate multikinase; D058570:TOR Serine-Threonine Kinases