title: TLR4 polymorphisms associated with developing gastric pre-cancer lesions in a Chinese Han population.
abstract: Helicobacter pylori infection is a risk factor for gastric cancer. In addition, toll-like receptor 4 (TLR4) plays a fundamental role in pathogen recognition and activation of innate immunity. This study investigated the association of TLR4 polymorphisms with a risk of intestinal metaplasia (IM) and intraepithelial neoplasia (IN) in a Chinese Han population. This study analyzed TLR4 gene polymorphisms in 333 patients (IM, 193 cases; IN, 140 cases) and 312 atypia-free controls in a Chinese Han population using a Taqman allelic discrimination assay. The TLR4 single nucleotide polymorphisms +896A/G and +1196C/T were not associated with the risk of IM or IN. However, the single-locus analysis showed that the C allele of TLR4+2856T/C had significantly reduced risk of IM and IN [adjusted odds ratio (OR)=0.42; 95%CI=0.29-0.62 and OR=0.62; 95%CI=0.41-0.93, respectively] compared with the wild-type homozygote (TT). The frequencies of TLR4+2856T/C TC and T carrier were significantly lower in patients with Sydney's slight IM and low grade IN (P<0.01 and P=0.01, respectively), while the TC genotype showed a lower risk of moderate IM compared to healthy controls (P=0.045). In addition, the data revealed that H. pylori infection, heavy alcohol consumption and high salt uptake were associated with a higher susceptibility for developing this neoplasm. TLR4 rs10759932 TC and C carriers were associated with a lower risk in developing precancerous lesions in the stomach in a Chinese Han population.
authors.affiliation: Department of Traditional Chinese Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Yao-fu
authors.initials: YF
authors.lastname: Fan
authors.affiliation: Department of Traditional Chinese Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China; Department of Gastroenterology, The Third People's Hospital of Wuxi, Wuxi 214041, Jiangsu, China.
authors.forename: Yan-min
authors.initials: YM
authors.lastname: Wu
authors.affiliation: Department of Traditional Chinese Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.affiliation: Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of General Surgery, Wuxi Higher Health Vocational Technology School, Wuxi 214028, China.
authors.forename: Yang-yang
authors.initials: YY
authors.lastname: Jiang
authors.affiliation: Department of Gastroenterology, The Third People's Hospital of Wuxi, Wuxi 214041, Jiangsu, China.
authors.forename: Yu-zheng
authors.initials: YZ
authors.lastname: Xue
authors.affiliation: Department of Gastroenterology, The Third People's Hospital of Wuxi, Wuxi 214041, Jiangsu, China.
authors.forename: Zong-liang
authors.initials: ZL
authors.lastname: Liu
authors.affiliation: Department of Traditional Chinese Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China. Electronic address: weimuxin@njmu.edu.cn.
authors.forename: Mu-Xin
authors.initials: MX
authors.lastname: Wei
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002681:China; D018450:Disease Progression; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D016481:Helicobacter Infections / Q000150:complications / Q000235:genetics / Q000276:immunology*; D016480:Helicobacter pylori / Q000502:physiology*; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D007413:Intestinal Mucosa / Q000502:physiology*; D007422:Intestines / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009369:Neoplasms; D020641:Polymorphism, Single Nucleotide; D012306:Risk; D013274:Stomach Neoplasms / Q000209:etiology / Q000235:genetics / Q000276:immunology*; D051197:Toll-Like Receptor 4 / Q000235:genetics*; D055815:Young Adult
chemical_list: C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4