title: Regeneration of plants from mesophyll protoplasts of the wild crucifer Eruca sativa Lam.
abstract: Protoplasts isolated from mesophyll cells of Eruca sativa Lam., cultured on suitable medium, underwent sustained cell divisions to form calli. The plating efficiency was found to be 0.4%. The protoplast-derived calli subsequently produced plantlets through organogenesis (15.71%) and somatic embryogenesis (11.25%). Regenerated plants exhibited normal appearance. These results indicate potential to introgress desirable traits from this wild crucifer into important oilseed and cole Brassicas by protoplast fusion and hybrid recovery.
authors.affiliation: Programme in Genetical Research, Bose Institute, P1/12, C.I.T. Scheme VII-M, 700 054, Calcutta, India.
authors.forename: S R
authors.initials: SR
authors.lastname: Sikdar
authors.forename: G
authors.initials: G
authors.lastname: Chatterjee
authors.forename: S
authors.initials: S
authors.lastname: Das
authors.forename: S K
authors.initials: SK
authors.lastname: Sen