title: Chromosomal damage in peripheral blood lymphocytes from nurses occupationally exposed to chemicals.
abstract: In the present study, we evaluated the induced genome damage in peripheral blood lymphocytes from a sample of nurses occupationally exposed to low doses of different chemicals. A comprehensive multi-biomarker approach using cytogenetic endpoints was employed for analyzing chromosomal aberrations (CAs) and sister chromatid exchange (SCE) assay. The study included 20 nurses and 20 control subjects matched in age, gender and smoking habits. Nurses were exposed to different chemicals, such as cytostatic drugs, anaesthetics, formaldehyde and other sterilizing gases. Significant differences were found between exposure group and control group in terms of SCEs frequency (p < 0.001) but not in terms of replication index value (p = 0.845) and CAs (p = 0.236). Regression analyses indicated that the age and the exposure years did not influence the amount of the chromosomal damage among nurses. Vice versa, among controls, a positive correlation was found between the number of SCEs and age. In conclusion, our results suggest that a continuous long-term exposure to low doses of chemicals could result in increased levels of SCEs among nurses. This data emphasize the importance of biomonitoring nurses and other hospital workers handling drugs.
authors.affiliation: Department of Life Sciences and Systems Biology, University of Turin, Torino, Italy alfredo.santovito@unito.it.
authors.forename: A
authors.initials: A
authors.lastname: Santovito
authors.affiliation: Department of Life Sciences and Systems Biology, University of Turin, Torino, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Cervella
authors.affiliation: Department of Life Sciences and Systems Biology, University of Turin, Torino, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Delpero
mesh_terms: D000328:Adult; D000777:Anesthetics / Q000633:toxicity*; D000970:Antineoplastic Agents / Q000633:toxicity*; D002869:Chromosome Aberrations / Q000139:chemically induced*; D004202:Disinfectants; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects; D008875:Middle Aged; D009726:Nurses; D016273:Occupational Exposure; D011481:Protective Clothing; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013997:Time Factors; D055815:Young Adult
chemical_list: D000777:Anesthetics; D000970:Antineoplastic Agents; D004202:Disinfectants
keywords: Nurses; SCE assay; chromosomal aberrations; occupational exposure