title: Human umbilical mesenchymal stem cells-seeded bladder acellular matrix grafts for reconstruction of bladder defects in a canine model.
abstract: BACKGROUND
  The goal of this study was to explore the feasibility of utilizing human umbilical mesenchymal stem cells (HUMSCs)-seeded Bladder acellular matrix graft (BAMG) for bladder reconstruction in a canine model.
  
  
  RESULTS
  HUMSCs were isolated from newborn umbilical cords and identified by flow cytometry. Partial cystectomy was performed in the experimental and control group. Bladder defects were repaired with HUMSCs-BAMG in the experimental group and repaired with unseeded-BAMG in control group. The implanted grafts were harvested after surgery. H&E and immunohistochemistry staining were performed to evaluate the regeneration of the bladder defect. Primary cultured HUMSCs displayed typical fibroblast morphology with spindle-shaped. Flow cytometry indicated that these cells were positive for CD105 (97.3%) and CD44 (99%), but negative for CD34 (2.8%), CD31 (2.1%), and CD45 (1.7%). Immunohistochemistry staining showed that a multilayered urothelium and well-developed smooth muscle were observed at 12 weeks in experiment group. In contrast, multilayered urothelial tissues were also observed at 12 weeks in group B, but well-developed smooth muscle bundles were observed.
  
  
  CONCLUSIONS
  Our preliminary results demonstrate that UMSC-seeded BAMGs are superior to unseeded BAMGs to promote the regeneration of bladder defects. Our findings indicated that HUMSCs may be a potential cell source for bladder tissue engineering.
authors.affiliation: Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan, P.R. China.
authors.forename: Haichao
authors.initials: H
authors.lastname: Yuan
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhuang
authors.forename: Ju
authors.initials: J
authors.lastname: Xiong
authors.forename: Wei
authors.initials: W
authors.lastname: Zhi
authors.forename: Liangren
authors.initials: L
authors.lastname: Liu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wei
authors.forename: Ping
authors.initials: P
authors.lastname: Han
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D018929:Cell Culture Techniques; D004285:Dogs; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D019651:Plastic Surgery Procedures; D012038:Regeneration; D054457:Tissue Scaffolds; D014470:Umbilical Cord / Q000166:cytology*; D001743:Urinary Bladder / Q000502:physiology*
chemical_list: D001672:Biocompatible Materials