title: Isolation and characterization of polysaccharides with the antitumor activity from Tuber fruiting bodies and fermentation system.
abstract: Fifty-two polysaccharides were isolated from the fermentation systems of Tuber melanosporum, Tuber indicum, Tuber sinense, Tuber aestivum and the fruiting bodies of Tuber indicum, Tuber himalayense, Tuber sinense by elution with an activated carbon column. Polysaccharides from Tuber fermentation system exhibited relatively higher in vitro antitumor activity against HepG2, A549, HCT-116, SK-BR-3, and HL-60 cells than those from Tuber fruiting bodies. All polysaccharides were mainly composed of D-mannose, D-glucose, and D-galactose, which suggested that the polysaccharides from Tuber fruiting bodies and fermentation system have identical chemical compositions. The results of antitumor activity and structural identification indicated that the polysaccharide fractions could promote antitumor activity. Tuber polysaccharides from Tuber fermentation system exhibited relatively higher than that from Tuber fruiting bodies. These results confirm the potential of Tuber fermentation mycelia for use as an alternative resource for its fruiting bodies.
authors.affiliation: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan, 430068, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Wang
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Li
authors.forename: Shi-Hua
authors.initials: SH
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Zhan-Peng
authors.initials: ZP
authors.lastname: Yuan
authors.forename: Ya-Jie
authors.initials: YJ
authors.lastname: Tang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D001203:Ascomycota / Q000737:chemistry / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005285:Fermentation; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006801:Humans; D008358:Mannose / Q000032:analysis; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides; D005947:Glucose; D008358:Mannose; D005690:Galactose