title: Identification and characterization of ϕH111-1: A novel myovirus with broad activity against clinical isolates ofBurkholderia cenocepacia.
abstract: Characterization of prophages in sequenced bacterial genomes is important for virulence assessment, evolutionary analysis, and phage application development. The objective of this study was to identify complete, inducible prophages in the cystic fibrosis (CF) clinical isolateBurkholderia cenocepaciaH111. Using the prophage-finding program PHAge Search Tool (PHAST), we identified three putative intact prophages in the H111 sequence. Virions were readily isolated from H111 culture supernatants following extended incubation. Using shotgun cloning and sequencing, one of these virions (designated ϕH111-1 [vB_BceM_ϕH111-1]) was identified as the infective particle of a PHAST-detected intact prophage. ϕH111-1 has an extremely broad host range with respect toB. cenocepaciastrains and is predicted to use lipopolysaccharide (LPS) as a receptor. Bioinformatics analysis indicates that the prophage is 42,972 base pairs in length, encodes 54 proteins, and shows relatedness to the virion morphogenesis modules ofAcaML1 and "Vhmllikevirus" myoviruses. As ϕH111-1 is active against a broad panel of clinical strains and encodes no putative virulence factors, it may be therapeutically effective forBurkholderiainfections.
authors.affiliation: Department of Biological Sciences; University of Alberta; Edmonton, Alberta Canada.
authors.forename: Karlene H
authors.initials: KH
authors.lastname: Lynch
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Liang
authors.forename: Leo
authors.initials: L
authors.lastname: Eberl
authors.forename: David S
authors.initials: DS
authors.lastname: Wishart
authors.forename: Jonathan J
authors.initials: JJ
authors.lastname: Dennis
keywords: Burkholderia cepacia complex; PHAST; bioinformatics; phage therapy; prophage identification