title: Solirubrobacter phytolaccae sp. nov., an endophytic bacterium isolated from roots of Phytolacca acinosa Roxb.
abstract: A Gram-staining-positive, strictly aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated GTGR-8(T), which formed white colonies, was isolated from roots of Phytolacca acinosa Roxb. collected from Taibai Mountain in Shaanxi Province, north-west China. Strain GTGR-8(T) grew optimally at 28-30 °C, at pH 7.0-8.0 and in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GTGR-8(T) was a member of the genus Solirubrobacter and was closely related to Solirubrobacter pauli B33D1(T) (98.9% similarity), Solirubrobacter ginsenosidimutans BXN5-15(T) (97.0%) and Solirubrobacter soli Gsoil 355(T) (96.9%). No other recognized bacterial species showed more than 94.2% 16S rRNA gene sequence similarity to the novel isolate. The only respiratory quinone of strain GTGR-8(T) was MK-7(H4) and the major fatty acids (>5%) were iso-C16 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 3ω6c (6,9,12) and C17 : 1ω6c. The DNA G+C content was 71.0 mol%. DNA-DNA relatedness for strain GTGR-8(T) with respect to its closest relatives, S. pauli KCTC 9974(T) and S. ginsenosidimutans KCTC 19420(T), was 52.5 and 24.5%, respectively. Based on phenotypic, phylogenetic and genotypic data, strain GTGR-8(T) is considered to represent a novel species in the genus Solirubrobacter, for which the name Solirubrobacter phytolaccae sp. nov. is proposed. The type strain is GTGR-8(T) ( = CCTCC AB 2013011(T) = KCTC 29190(T)).
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Linfang
authors.initials: L
authors.lastname: Wei
authors.affiliation: Department of Gynecol&Obstet, Wuhan Central Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, Hubei, PR China.
authors.forename: Shan
authors.initials: S
authors.lastname: Ouyang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Xihui
authors.initials: X
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D029603:Phytolacca / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7