title: Solution structure of Rv0569, potent hypoxic signal transduction protein, from Mycobacterium tuberculosis.
abstract: The latent infection is unique characteristic of Mycobacterium tuberculosis to overcome human immune response for its survival. The M. tb develops adaptation to extreme stress conditions to increase the viability, thus easily acquires drug resistance than any other bacteria and maintains a long-term infection status without any symptoms. Rv0569 is a conserved hypothetical protein that overexpresses under dormant state induced by hypoxia, starvation, and medication. To study function and structure in detail, we determined the solution structure of Rv0569 by NMR. NOE and RDC restraints were used to calculate the structure, which was further refined with AMBER. Rv0569 is composed of five antiparallel β-sheets and one α-helix. Rv0569 shows structural similarity with its homolog Rv2302, yet there is a big difference in the orientation of C-terminal α-helix between Rv0569 and Rv2302. According to previous studies, Rv0569 might comprise a hypoxia induced operon with the Rv0570 which is located 29 bp downstream of the Rv0569 and Rv0570 plays an important role in the latent infection. From our structure and bioinformatics research, we suggest that Rv0569 contributes to signaling transduction in hypoxic condition by binding with DNA for upregulation of Rv0570 or supporting Rv0570 for binding ATP during dormancy of tuberculosis.
authors.affiliation: College of Pharmacy, Seoul National University, Gwanak-Gu, Seoul 151-742, Republic of Korea.
authors.forename: Won-Je
authors.initials: WJ
authors.lastname: Kim
authors.affiliation: College of Pharmacy, CHA University, 120 Haeryong-ro, Pocheon-si, Gyeonggi-do 487-010, Republic of Korea.
authors.forename: Woo Sung
authors.initials: WS
authors.lastname: Son
authors.affiliation: College of Pharmacy, Seoul National University, Gwanak-Gu, Seoul 151-742, Republic of Korea.
authors.forename: Do-Hwan
authors.initials: DH
authors.lastname: Ahn
authors.affiliation: College of Pharmacy, Seoul National University, Gwanak-Gu, Seoul 151-742, Republic of Korea.
authors.forename: Hookang
authors.initials: H
authors.lastname: Im
authors.affiliation: College of Pharmacy, Dongguk University, 32 Dongguk-ro, Ilsandong-gu, Goyang-si 410-820, Republic of Korea.
authors.forename: Hee-Chul
authors.initials: HC
authors.lastname: Ahn
authors.affiliation: College of Pharmacy, Seoul National University, Gwanak-Gu, Seoul 151-742, Republic of Korea. Electronic address: lbj@nmr.snu.ac.kr.
authors.forename: Bong-Jin
authors.initials: BJ
authors.lastname: Lee
mesh_terms: D000222:Adaptation, Physiological; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D000860:Hypoxia; D055985:Latent Tuberculosis / Q000276:immunology / Q000382:microbiology*; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000378:metabolism* / Q000472:pathogenicity; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D020539:Sequence Analysis, Protein; D015398:Signal Transduction; D015854:Up-Regulation
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C000592393:Rv0569 protein, Mycobacterium tuberculosis; D000429:Alcohol Oxidoreductases; C096794:C-terminal binding protein
keywords: Dormancy; Hypoxic signal transduction; Mycobacterium tuberculosis; Rv0569; Solution structure