title: Intersex condition and molecular markers of endocrine disruption in relation with burdens of emerging pollutants in thicklip grey mullets (Chelon labrosus) from Basque estuaries (South-East Bay of Biscay).
abstract: Endocrine disrupting chemicals (EDCs) interfere with the functioning of the endocrine system, causing reproductive and developmental disturbances in aquatic wildlife. Appearance of intersex gonads and elevated plasma levels of vitellogenin in male fish are well known biomarkers of exposure to xenoestrogenic EDCs. In the present study, intersex condition and transcription levels of vtg and cyp19a1b were assessed in five thicklip grey mullet populations from the Basque coast (Bay of Biscay). Levels of EDCs (estrogenic hormones, polycyclic musks, bisphenol-A, phthalates, alkylphenols and pesticides) were determined in water and fish bile. Intersex gonads were observed in three out of five mullet populations. Vtg and cyp19a1b were up-regulated in mullet populations with relatively higher EDCs load. Phthalates and pesticides were the most abundant EDCs in bile, followed by alkylphenols, musks, bisphenol-A and estrogenic hormones. Statistically significant correlations were found between concentrations of individual and total EDCs in bile and water samples and transcription levels of vtg and cyp19a1b.
authors.affiliation: Dep. Zoology and Cell Biology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Sarriena z/g, E-48940 Leioa, Basque Country, Spain; Research Centre for Experimental Marine Biology and Biotechnology (PIE), UPV/EHU, Areatza z/g, E-48620 Plentzia, Basque Country, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Bizarro
authors.affiliation: Dep. Analytical Chemistry, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Sarriena z/g, E-48940 Leioa, Basque Country, Spain.
authors.forename: O
authors.initials: O
authors.lastname: Ros
authors.affiliation: Dep. Analytical Chemistry, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Sarriena z/g, E-48940 Leioa, Basque Country, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Vallejo
authors.affiliation: Dep. Analytical Chemistry, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Sarriena z/g, E-48940 Leioa, Basque Country, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Prieto
authors.affiliation: Research Centre for Experimental Marine Biology and Biotechnology (PIE), UPV/EHU, Areatza z/g, E-48620 Plentzia, Basque Country, Spain; Dep. Analytical Chemistry, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Sarriena z/g, E-48940 Leioa, Basque Country, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Etxebarria
authors.affiliation: Dep. Zoology and Cell Biology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Sarriena z/g, E-48940 Leioa, Basque Country, Spain; Research Centre for Experimental Marine Biology and Biotechnology (PIE), UPV/EHU, Areatza z/g, E-48620 Plentzia, Basque Country, Spain.
authors.forename: M P
authors.initials: MP
authors.lastname: Cajaraville
authors.affiliation: Dep. Zoology and Cell Biology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Sarriena z/g, E-48940 Leioa, Basque Country, Spain; Research Centre for Experimental Marine Biology and Biotechnology (PIE), UPV/EHU, Areatza z/g, E-48620 Plentzia, Basque Country, Spain. Electronic address: maren.ortiz@ehu.es.
authors.forename: M
authors.initials: M
authors.lastname: Ortiz-Zarragoitia
mesh_terms: D000818:Animals; D001141:Aromatase / Q000235:genetics / Q000378:metabolism; D001646:Bile / Q000737:chemistry; D015415:Biomarkers / Q000378:metabolism*; D012734:Disorders of Sex Development / Q000139:chemically induced* / Q000378:metabolism / Q000503:physiopathology; D052244:Endocrine Disruptors / Q000633:toxicity*; D004781:Environmental Exposure; D004784:Environmental Monitoring; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D006066:Gonads / Q000187:drug effects / Q000503:physiopathology; D008297:Male; D023701:Smegmamorpha; D013030:Spain; D014819:Vitellogenins / Q000235:genetics / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D015415:Biomarkers; D052244:Endocrine Disruptors; D029941:Fish Proteins; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D001141:Aromatase
keywords: Aromatase cyp19a1b; Bay of Biscay; Bile analytes; Chelon labrosus; Endocrine disrupting chemicals; Fish; Intersex; Vitellogenin; Water chemistry