title: Comparison of a capillary gel electrophoresis-based multiplex PCR assay and ribosomal intergenic transcribed spacer-2 amplicon sequencing for identification of clinically important Candida species.
abstract: The performance of a commercially available Seegene Seeplex STI Master Panel 3 multiplex PCR for Candida species identification was compared with an internal transcribed spacer 2 (ITS2) PCR assay. We found that the Seeplex assay was specific for identification of C. albicans, C. krusei, C. parapsilosis, C. glabrata, C. tropicalis and C. dubliniensis.
authors.affiliation: Division of Clinical Microbiology, Department of Experimental Medicine, Faculty of Health Sciences, Linköping University, County Council of Östergötland, S-581 85 Linköping, Sweden. Electronic address: hans-jurg.monstein@liu.se.
authors.forename: Hans-Jürg
authors.initials: HJ
authors.lastname: Monstein
authors.affiliation: Division of Clinical Microbiology, Department of Experimental Medicine, Faculty of Health Sciences, Linköping University, County Council of Östergötland, S-581 85 Linköping, Sweden.
authors.forename: Maria
authors.initials: M
authors.lastname: Tärnberg
authors.affiliation: Division of Clinical Microbiology, Department of Experimental Medicine, Faculty of Health Sciences, Linköping University, County Council of Östergötland, S-581 85 Linköping, Sweden.
authors.forename: Shohreh
authors.initials: S
authors.lastname: Persis
authors.affiliation: Division of Clinical Microbiology, Department of Experimental Medicine, Faculty of Health Sciences, Linköping University, County Council of Östergötland, S-581 85 Linköping, Sweden.
authors.forename: Anders G
authors.initials: AG
authors.lastname: Johansson
mesh_terms: D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002177:Candidiasis / Q000382:microbiology*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics*; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer
keywords: Candida sp. internal transcribed spacer-2 (ITS2)-PCR; Candida specific multiplex PCR; Capillary gel electrophoresis