title: An optical sensing strategy leading to in situ monitoring of the degradation of mesoporous magnetic supraparticles in cells.
abstract: Mesoporous magnetic supraparticles (meso-MSPs) as multifunctional targeted drug carriers have attracted much attention, because of their easy magnetic-field manipulation and in situ sensing functionality. In this paper, a Fe(3+)-selective chemodosimeter fluorescent probe (FP-1) was synthesized and loaded inside of the meso-MSPs (meso-MSPs/probe); the meso-MSPs/probe nanocomposites were then used to monitor the degradation of meso-MSPs in cells. In our experiments, strong fluorescence intensity was observed in HeLa cells, because of their acidic intracellular environment, which can quickly degrade the meso-MSPs and then release Fe(3+) ions in cells that, in turn, activate the fluorescence of FP-1. Meanwhile, a very weak fluorescence signal was detected in HEK 293T cells due to the relative neutral intracellular environment of HEK 293T cells, which prevented the Fe(3+) ion from leaching out of meso-MSPs. Moreover, this degradation-luminescence relationship of the meso-MSPs/probe nanocomposites not only assisted us to understand the degradation status of meso-MSPs in cells, but also allowed us to recognize the peculiarity of different cells with various intracellular environments.
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, and Laboratory of Advanced Materials, Fudan University , Shanghai, 200433, People's Republic of China.
authors.forename: Dian
authors.initials: D
authors.lastname: Li
authors.forename: Yuting
authors.initials: Y
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Yang
authors.forename: Meng
authors.initials: M
authors.lastname: Yu
authors.forename: Jia
authors.initials: J
authors.lastname: Guo
authors.forename: Jennifer Q
authors.initials: JQ
authors.lastname: Lu
authors.forename: Changchun
authors.initials: C
authors.lastname: Wang
mesh_terms: D005456:Fluorescent Dyes; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008280:Magnetics; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D055095:Optics and Photonics
chemical_list: D005456:Fluorescent Dyes