title: Study of drug resistance and molecular typing of 59 cholerae01 clinical isolates from 1984 to 2002 in Chongqing, China.
abstract: OBJECTIVE
  To analyze the correlation between drug resistance and Cholerae01 clinical isolates from 1984 to 2002 in Chongqing, China.
  
  
  METHODS
  K-B assay was applied to detect the sensitivity of 59 Cholerae01 clinical isolates (20 Ogawa, 39 Inaba) to 16 kinds of antibiotics. BioNumerics software was used for a cluster analysis of electrophoresis patterns obtained from the Not I enzyme-cutting genomic DNA by Pulsed-field gel electrophoresis (PFGE).
  
  
  RESULTS
  Vibrio cholerae01 in Chongqing area, China were highly resistant to Cotrimoxazole, Furazolidone and Streptomycin. The resistance rates were 28.81% (17/59), 61.02% (36/59) and 30.51% (18/59), respectively. While the isolates from the crowd were sensitive to Amikacin, Gentamicin, Tobramycin, Ampicillin, Neomycin and Doxycycline, and no drug-resistant strains were observed.
  
  
  CONCLUSIONS
  No significant changes are found in the drug resistance of Vibrio cholerae01 from the crowd in Chongqing, China and the drug resistances of the Ogawa and the Inaba strains are different. Vibrio cholerae01 from the crowd in Chongqing, China are highly homologous, which may be from the epidemic strains with the same source.
authors.affiliation: Center for Disease Control and Prevention No.8 Changjiang 2nd Rd, Yuzhong District, 400042, Chongqing, China.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Li
authors.forename: Wenzen
authors.initials: W
authors.lastname: Wang
authors.forename: Gang
authors.initials: G
authors.lastname: Duan
authors.forename: Chunyan
authors.initials: C
authors.lastname: Lian
authors.forename: Hua
authors.initials: H
authors.lastname: Ling
authors.forename: Bo
authors.initials: B
authors.lastname: Zhao
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Yuan Min
authors.initials: YM
authors.lastname: Wang
authors.forename: Liangui
authors.initials: L
authors.lastname: Feng
keywords: Vibrio cholerae01; drug-resistance; molecular typing