title: Human blood plasma proteome mapping for search of potential markers of the lung squamous cell carcinoma.
abstract: Blood plasma proteomes obtained from 77 lung squamous cell carcinoma (LSCC) patients (Stages I-III) and 67 healthy controls (all males) were analyzed by using the label-free liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the search of potential cancer biomarkers. All plasma samples were depleted of 14 highly-abundant plasma proteins by immune-affinity column chromatography before LC-MS/MS. We identified and quantified 809 differential proteins with molecular weights from 6.4 kDa to 3900 kDa using a label-free method. Three hundred and sixty four proteins were identified in all three groups. Changes in levels of an expression of blood plasma proteins associated with LSCC were discovered. Among them, 43 proteins were overexpressed and 39 proteins were down-regulated by more than two-fold between the plasmas of lung cancer patients and healthy men. We focused our attention on proteins whose expression levels increased from control to early stage and then to advanced stage tumor. Each of the 43 unique overexpressed proteins was classified according to its cellular localization, biological processes, molecular function and classes. Many of these proteins are involved in biological pathways pertinent to tumor progression and metastasis and some of these deregulated proteins may be useful clinical markers.
authors.affiliation: Federal State Budgetary Institution N. N. Btokhin Russian Cancer Research Center Under the Russian Academy of Medical Science, Moscow, Russia. vshev@nm.ru
authors.forename: Valeriy E
authors.initials: VE
authors.lastname: Shevchenko
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Kovalev
authors.forename: Natalia E
authors.initials: NE
authors.lastname: Arnotskaya
authors.forename: Irina B
authors.initials: IB
authors.lastname: Zborovskaya
authors.forename: Bakhrom B
authors.initials: BB
authors.lastname: Akhmedov
authors.forename: Boris E
authors.initials: BE
authors.lastname: Polotskii
authors.forename: Aleksey U
authors.initials: AU
authors.lastname: Kostin
authors.forename: Anush F
authors.initials: AF
authors.lastname: Moukeria
authors.forename: David G
authors.initials: DG
authors.lastname: Zaridze
authors.forename: Mikhail I
authors.initials: MI
authors.lastname: Davidov
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000737:chemistry* / Q000235:genetics*; D001798:Blood Proteins / Q000737:chemistry* / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D030562:Databases, Protein; D015536:Down-Regulation; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry* / Q000235:genetics*; D008875:Middle Aged; D008970:Molecular Weight; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011492:Protein Hydrolysates / Q000737:chemistry; D020543:Proteome / Q000235:genetics*; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000737:chemistry
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins; D011492:Protein Hydrolysates; D020543:Proteome; D014357:Trypsin