title: Optimization for production of exopolysaccharides with antitumor activity in vitro from Paecilomyces hepiali.
abstract: In the present study, optimal medium for the growth of mycelia and the production of exopolysaccharides from Paecilomyces hepiali HN1 (PHEPS) in submerged culture was investigated. As a result, the maximum production of mycelia (12.98 ± 0.14 g/L) and PHEPS (5.33 ± 0.11 g/L) were achieved under the optimal medium of sucrose 46.08 g/L, yeast extract 4.71 g/L, (NH₄)₂SO₄ 5.72 g/L, KH₂PO₄ 1.70 g/L, CaCl₂ 0.50 g/L, MgSO₄ 0.50 g/L, potato extract 1% and malt extract 1%. Furthermore, the antitumor activity of PHEPS in vitro was evaluated by using three cell lines of human liver tumor HepG2 cells, breast cancer MCF-7 cells and cervical cancer Hela cells. It was found that PHEPS exhibited relative higher anti-proliferative activity against HepG2 cells than MCF-7 cells and Hela cells. At a concentration of 500 μg/mL and 72 h treatment, the inhibition rate of PHEPS on HepG2 cells reached to 62.58%. All these results suggested that PHEPS could be explored as novel natural antitumor agent with great potential application.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China; College of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, PR China.
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Wu
authors.forename: Junwen
authors.initials: J
authors.lastname: Lu
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Wang
authors.forename: Bing
authors.initials: B
authors.lastname: Hu
authors.forename: Hong
authors.initials: H
authors.lastname: Ye
authors.forename: Jialong
authors.initials: J
authors.lastname: Fan
authors.forename: Muhammad
authors.initials: M
authors.lastname: Abid
authors.forename: Xiaoxiong
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D045424:Complex Mixtures / Q000378:metabolism; D003470:Culture Media; D005163:Factor Analysis, Statistical; D005260:Female; D005285:Fermentation; D062610:Fungal Polysaccharides / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D025282:Mycelium / Q000737:chemistry / Q000378:metabolism*; D010143:Paecilomyces / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D011198:Solanum tuberosum / Q000737:chemistry; D013395:Sucrose / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D045424:Complex Mixtures; D003470:Culture Media; D062610:Fungal Polysaccharides; D013395:Sucrose
keywords: Antitumor activity; Optimization; Paecilomyces hepiali HN1; Polysaccharides