title: Plant regeneration from in vitro cultures of anthers and mature seeds of rice (Oryza sativa L.) cv. Basmati-370.
abstract: Pollen plants were obtained from anther-derived calli of the indica rice variety Basmati-370. Anther-response (anthers producing pollen derived calli) and plant regeneration frequency from the pollen derived calli. was very low. Donor plants which flowered at the average max/min. temperature of 34.2°/23.3°C gave a significantly higher anther-response to in vitro techniques, than did those which flowered at 29.1°/16.4°C. Somatic callus induction and subsequent plant regeneration was readily obtained from mature seed embryos. While 2,4-D or 2,4,5-T (1 or 2 mg/l) proved highly efficient for callus induction, tryptophan (50 or 100 mg/l) induced a high frequency of green plants from the calli.
authors.affiliation: Biotechnology Centre, I.A.R.L., 110012, New Delhi, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Raina
authors.forename: P
authors.initials: P
authors.lastname: Sathish
authors.forename: K S
authors.initials: KS
authors.lastname: Sarma