title: Detecting a single molecule using a micropore-nanopore hybrid chip.
abstract: Nanopore-based DNA sequencing and biomolecule sensing have attracted more and more attention. In this work, novel sensing devices were built on the basis of the chips containing nanopore arrays in polycarbonate (PC) membranes and micropores in Si3N4 films. Using the integrated chips, the transmembrane ionic current induced by biomolecule's translocation was recorded and analyzed, which suggested that the detected current did not change linearly as commonly expected with increasing biomolecule concentration. On the other hand, detailed translocation information (such as translocation gesture) was also extracted from the discrete current blockages in basic current curves. These results indicated that the nanofluidic device based on the chips integrated by micropores and nanopores possessed comparative potentials in biomolecule sensing.
authors.affiliation: Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanics, Southeast University, Nanjing 210096, People's Republic of China. liulei@seu.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Lizhong
authors.initials: L
authors.lastname: Zhu
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Ni
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Chen