title: Trypsin-catalyzed tandem reaction: one-pot synthesis of 3,4-dihydropyrimidin-2(1H)-ones by in situ formed acetaldehyde.
abstract: A simple, mild, one-pot tandem method catalyzed by trypsin was developed for the synthesis of 3,4-dihydropyrimidin-2(1H)-ones by the Biginelli reaction of urea, β-dicarbonyl compounds, and in situ-formed acetaldehyde. Trypsin was found to display dual promiscuous functions to catalyze transesterification and the Biginelli reaction in sequence.
authors.affiliation: Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, China; School of Chemistry, Biology and Material Science, East China Institute of Technology, Fuzhou 344000, China(1). Electronic address: zbxie@ecit.edu.cn.
authors.forename: Zong-Bo
authors.initials: ZB
authors.lastname: Xie
authors.affiliation: Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, China. Electronic address: wnchem@scu.edu.cn.
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.affiliation: Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, China.
authors.forename: Wan-Xia
authors.initials: WX
authors.lastname: Wu
authors.affiliation: School of Chemistry, Biology and Material Science, East China Institute of Technology, Fuzhou 344000, China(1).
authors.forename: Zhang-Gao
authors.initials: ZG
authors.lastname: Le
authors.affiliation: Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, China. Electronic address: xqyu@scu.edu.cn.
authors.forename: Xiao-Qi
authors.initials: XQ
authors.lastname: Yu
mesh_terms: D000079:Acetaldehyde / Q000737:chemistry*; D055162:Biocatalysis; D004951:Esterification; D008956:Models, Chemical; D011744:Pyrimidinones / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism*
chemical_list: D011744:Pyrimidinones; D014357:Trypsin; D000079:Acetaldehyde
keywords: Biginelli reaction; In situ; One-pot; Promiscuity; Tandem protocol