title: Simultaneous detection of multiple hydroxylated polychlorinated biphenyls from a complex tissue matrix using gas chromatography/isotope dilution mass spectrometry.
abstract: In this study, we developed a comprehensive, highly sensitive, and robust method for determining 53 congeners of three to eight chlorinated OH-PCBs in liver and brain samples by using isotope dilution gas chromatography (GC) coupled with electron capture negative ionization mass spectrometry (ECNI-MS). These results were compared with those from GC coupled with electron ionization high-resolution mass spectrometry (EI-HRMS). Clean-up procedures for analysis of OH-PCBs homologs in liver and brain samples involve a pretreatment step consisting of acetonitrile partition and 5% hydrated silica-gel chromatography before derivatization. Recovery rates of tri- and tetra-chlorinated OH-PCBs in the acetonitrile partition method followed by the 5% hydrated silica-gel column (82% and 91%) were higher than conventional sulfuric acid treatment (2.0% and 3.5%). The method detection limits of OH-PCBs for each matrix obtained by GC/ECNI-MS and GC/EI-HRMS were 0.58-2.6 pg g(-1) and 0.36-1.6 pg g(-1) wet wt, respectively. Recovery rates of OH-PCB congeners in spike tests using sample matrices (10 and 50 pg) were 64.7-117% (CV: 4.7-14%) and 70.4-120% (CV: 2.3-12%), respectively. This analytical method may enable the simultaneous detection of various OH-PCBs from complex tissue matrices. Furthermore, this method allows more comprehensive assessment of the biological effects of OH-PCB exposure on critical organs.
authors.affiliation: Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 2-5, Matsuyama 790-8577, Japan.
authors.forename: Akifumi
authors.initials: A
authors.lastname: Eguchi
authors.forename: Kei
authors.initials: K
authors.lastname: Nomiyama
authors.forename: Mari
authors.initials: M
authors.lastname: Ochiai
authors.forename: Hazuki
authors.initials: H
authors.lastname: Mizukawa
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Nagano
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Kouki
authors.initials: K
authors.lastname: Tanaka
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Miyagawa
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Tanabe
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007201:Indicator Dilution Techniques; D008099:Liver / Q000378:metabolism*; D046049:Phoca / Q000378:metabolism*; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D019576:Porpoises / Q000378:metabolism*
chemical_list: D011078:Polychlorinated Biphenyls
keywords: Brain; Gas chromatography–mass spectrometry; Hydroxylated polychlorinated biphenyls; Liver