title: Quantitative phosphoproteomics reveals the role of protein arginine phosphorylation in the bacterial stress response.
abstract: Arginine phosphorylation is an emerging protein modification implicated in the general stress response of Gram-positive bacteria. The modification is mediated by the arginine kinase McsB, which phosphorylates and inactivates the heat shock repressor CtsR. In this study, we developed a mass spectrometric approach accounting for the peculiar chemical properties of phosphoarginine. The improved methodology was used to analyze the dynamic changes in the Bacillus subtilis arginine phosphoproteome in response to different stress situations. Quantitative analysis showed that a B. subtilis mutant lacking the YwlE arginine phosphatase accumulated a strikingly large number of arginine phosphorylations (217 sites in 134 proteins), however only a minor fraction of these sites was increasingly modified during heat shock or oxidative stress. The main targets of McsB-mediated arginine phosphorylation comprise central factors of the stress response system including the CtsR and HrcA heat shock repressors, as well as major components of the protein quality control system such as the ClpCP protease and the GroEL chaperonine. These findings highlight the impact of arginine phosphorylation in orchestrating the bacterial stress response.
authors.affiliation: Research Institute of Molecular Pathology - IMP, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria;
authors.forename: Andreas
authors.initials: A
authors.lastname: Schmidt
authors.forename: Débora Broch
authors.initials: DB
authors.lastname: Trentini
authors.forename: Silvia
authors.initials: S
authors.lastname: Spiess
authors.forename: Jakob
authors.initials: J
authors.lastname: Fuhrmann
authors.forename: Gustav
authors.initials: G
authors.lastname: Ammerer
authors.forename: Karl
authors.initials: K
authors.lastname: Mechtler
authors.forename: Tim
authors.initials: T
authors.lastname: Clausen
mesh_terms: D001120:Arginine / Q000031:analogs & derivatives* / Q000378:metabolism; D001122:Arginine Kinase / Q000378:metabolism*; D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006360:Heat-Shock Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D030781:Organisms, Genetically Modified; D009943:Organophosphorus Compounds / Q000378:metabolism; D010750:Phosphoproteins / Q000032:analysis / Q000378:metabolism*; D010766:Phosphorylation; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods; D013312:Stress, Physiological / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D009943:Organophosphorus Compounds; D010750:Phosphoproteins; D020543:Proteome; C015441:phospho-L-arginine; D001120:Arginine; D001122:Arginine Kinase