title: Potential of a Saccharomyces cerevisiae recombinant strain lacking ethanol and glycerol biosynthesis pathways in efficient anaerobic bioproduction.
abstract: Saccharomyces cerevisiae shows high growth activity under low pH conditions and can be used for producing acidic chemicals such as organic acids as well as fuel ethanol. However, ethanol can also be a problematic by-product in the production of chemicals except for ethanol. We have reported that a stable low-ethanol production phenotype was achieved by disrupting 6 NADH-dependent alcohol dehydrogenase genes of S. cerevisiae. Moreover, the genes encoding the NADH-dependent glycerol biosynthesis enzymes were further disrupted because the ADH-disrupted recombinant strain showed high glycerol production to maintain intracellular redox balance. The recombinant strain incapable producing ethanol and glycerol could have the potential to be a host for producing metabolite(s) whose biosynthesis is coupled with NADH oxidation. Indeed, we successfully achieved almost 100% yield for L-lactate production using this recombinant strain as a host. In addition, the potential of our constructed recombinant strain for efficient bioproduction, particularly under anaerobic conditions, is also discussed.
authors.affiliation: Department of Bioengineering; Tokyo Institute of Technology; Kanagawa, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Hirasawa
authors.affiliation: Department of Bioinformatic Engineering; Graduate School of Information Science and Technology; Osaka University; Osaka, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ida
authors.affiliation: Quantitative Biology Center; RIKEN; Osaka, Japan.
authors.forename: Chikara
authors.initials: C
authors.lastname: Furuasawa
authors.affiliation: Department of Bioinformatic Engineering; Graduate School of Information Science and Technology; Osaka University; Osaka, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimizu
mesh_terms: D000426:Alcohol Dehydrogenase / Q000502:physiology*; D000693:Anaerobiosis / Q000502:physiology; D000431:Ethanol / Q000378:metabolism*; D020868:Gene Silencing; D024861:Genetic Enhancement / Q000379:methods*; D005990:Glycerol / Q000378:metabolism*; D019344:Lactic Acid / Q000096:biosynthesis*; D010100:Oxygen / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000502:physiology*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D019344:Lactic Acid; D000431:Ethanol; D000426:Alcohol Dehydrogenase; D005990:Glycerol; D010100:Oxygen
keywords: L-lactate; Saccharomyces cerevisiae; alcohol dehydrogenase; bioproduction; ethanol; glycerol