title: Transformation of protoplast-derived cell colonies and suspension cultures by Agrobacterium tumefaciens.
abstract: In this paper we describe procedures for transforming micro colonies derived from mesophyll protoplasts of Petunia hybrida with Agrobacterium tumefaciens. The method is efficient, up to 70% of the colonies were transformed, and we used a similar method to transform cells from a suspension culture of haploid Nicotiana plumbaginifolia.
authors.affiliation: Unilever Research, Colworth House, MK44 1LQ, Sharnbrook, Bedfordshire, UK.
authors.forename: K
authors.initials: K
authors.lastname: Pollock
authors.forename: D G
authors.initials: DG
authors.lastname: Barfield
authors.forename: S J
authors.initials: SJ
authors.lastname: Robinson
authors.forename: R
authors.initials: R
authors.lastname: Shields