title: Isolation of phosphate-solubilizing fungus and its application in solubilization of rock phosphates.
abstract: Microorganisms have been obtained to improve the agronomic value of rock phosphates (RPs), but the phosphorus solubilizing rate by these approaches is very slow. It is important to explore a high-efficient phosphate-solubilizing approach with a kind of microorganisms. This study aimed to isolate a high-efficient level of phosphate-solubilizing fungus from rhizosphere soil samples phosphate mines (Liuyang County, Hunan province, China) and apply it in solubilization of RPs. The experiments were carried out by the conventional methodology for morphological and biochemical fungus characterization and the analysis of 18s rRNA sequence. Then the effects of time, temperature, initial pH, phosphorus (P) sources, RPs concentration, shaking speed and silver ion on the content of soluble P released by this isolate were investigated. The results showed this isolate was identified as Galactomyces geotrichum P14 (P14) in GeneBank and the maximum amount of soluble P was 1252.13 mg L(-1) within 40 h in a modified phosphate growth agar's medium (without agar) where contained tricalcium phosphate (TCP) as sole phosphate source. At the same time, it could release phosphate and solubilize various rock phosphates. The isolated fungus can convert RPs from insoluble form into plant available form and therefore it hold great potential for biofertilizers to enhance soil fertility and promote plant growth.
authors.affiliation: Department of Environmental Microbiology, Hunan Institute of Microbiology, Changsha, China.
authors.forename: Yingben
authors.initials: Y
authors.lastname: Wu
authors.forename: Yuelin
authors.initials: Y
authors.lastname: He
authors.forename: Hongmei
authors.initials: H
authors.lastname: Yin
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Xu
authors.forename: Aiqun
authors.initials: A
authors.lastname: Zhang
mesh_terms: D002130:Calcium Phosphates / Q000378:metabolism; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008906:Mining; D010710:Phosphates / Q000378:metabolism*; D010802:Phylogeny; D058441:Rhizosphere; D021521:Ribotyping; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D012995:Solubility; D013696:Temperature; D013995:Time
chemical_list: D002130:Calcium Phosphates; D010710:Phosphates; D012989:Soil Pollutants; C018392:tricalcium phosphate