title: [The correlation of synuclein-γ and matrix metalloproteinase 9 in breast cancer].
abstract: OBJECTIVE
  To evaluate the expression of synuclein-γ (SNCG) and metalloproteinase 9 (MMP-9) both in the invasive ductal breast cancer samples and T47D and T47D(SNCG)- cell lines, to investigate the correlation between SNCG and MMP-9.
  
  
  METHODS
  Totally 96 invasive ductal breast cancer samples (female, mean age of (56 ± 8) years) were collected between June 2009 and June 2012. The expressions of SNCG and MMP-9 were investigated by immunohistochemistry. T47D and SNCG knock down T47D(SNCG)- cell lines were established and SNCG and MMP-9 protein expression were investigated by Western blot and gene expression by real-time PCR.
  
  
  RESULTS
  Among 96 samples, 26 (27.1%) of them co-expressed SNCG and MMP-9, 30(31.2%) of them expressed neither SNCG nor MMP-9. The expression of SNCG was correlated with the expression of MMP-9 (r = 0.655, P = 0.000).SNCG mRNA level of T47D cell line was 13.5 fold of T47D(SNCG)- cell line and SNCG protein expression was 2.1 fold. While MMP-9 mRNA level of T47D cell line was 7.3 fold of T47D(SNCG)- cell line and MMP-9 protien expression was 1.6 fold.When SNCG was knocked down, the expression of MMP-9 decreased.
  
  
  CONCLUSIONS
  SNCG and MMP-9 are significantly correlated with each other in breast cancer. SNCG may promote the invasion and metastasis of breast cancer mediated by up-regulating the expression of MMP-9.
authors.affiliation: Department of General Surgery, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Shuo
authors.initials: S
authors.lastname: Huang
authors.forename: Ke-jin
authors.initials: KJ
authors.lastname: Wu
authors.forename: Yong-kun
authors.initials: YK
authors.lastname: Wang
authors.forename: Yi-jun
authors.initials: YJ
authors.lastname: Jia
authors.forename: Yun-shu
authors.initials: YS
authors.lastname: Lu
authors.forename: Zi-yi
authors.initials: ZY
authors.lastname: Weng
mesh_terms: D000368:Aged; D001943:Breast Neoplasms / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D051845:gamma-Synuclein / Q000378:metabolism*
chemical_list: D009363:Neoplasm Proteins; D012333:RNA, Messenger; C104249:SNCG protein, human; D051845:gamma-Synuclein; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9