title: Hsa-let-7g miRNA targets caspase-3 and inhibits the apoptosis induced by ox-LDL in endothelial cells.
abstract: It has been well confirmed ox-LDL plays key roles in the development of atherosclerosis via binding to LOX-1 and inducing apoptosis in vascular endothelial cells. Recent studies have shown ox-LDL can suppress microRNA has-let-7g, which in turn inhibits the ox-LDL induced apoptosis. However, details need to be uncovered. To determine the anti-atherosclerosis effect of microRNA has-let-7g, and to evaluate the possibility of CASP3 as an anti-atherosclerotic drug target by has-let-7g, the present study determined the role of hsa-let-7g miRNA in ox-LDL induced apoptosis in the vascular endothelial cells. We found that miRNA has-let-7g was suppressed during the ox-LDL-induced apoptosis in EAhy926 endothelial cells. In addition, overexpression of has-let-7g negatively regulated apoptosis in the endothelial cells by targeting caspase-3 expression. Therefore, miRNA let-7g may play important role in endothelial apoptosis and atherosclerosis.
authors.affiliation: Emergency Department, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China. yfzhangzhd@163.com.
authors.forename: Yefei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Naiyun
authors.initials: N
authors.lastname: Chen
authors.forename: Jihao
authors.initials: J
authors.lastname: Zhang
authors.forename: Yaling
authors.initials: Y
authors.lastname: Tong
mesh_terms: D017209:Apoptosis / Q000235:genetics; D050197:Atherosclerosis / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D053148:Caspase 3 / Q000096:biosynthesis* / Q000235:genetics; D002460:Cell Line; D002470:Cell Survival / Q000235:genetics; D042783:Endothelial Cells / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D008077:Lipoproteins, LDL / Q000037:antagonists & inhibitors / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics*; D015398:Signal Transduction
chemical_list: D008077:Lipoproteins, LDL; D035683:MicroRNAs; C487931:mirnlet7 microRNA, human; C099252:oxidized low density lipoprotein; D053148:Caspase 3