title: IFN-γ inhibits basal and α-MSH-induced melanogenesis.
abstract: Inflammatory cytokines are closely related to pigmentary changes. In this study, the effects of IFN-γ on melanogenesis were investigated. IFN-γ inhibits basal and α-MSH-induced melanogenesis in B16 melanoma cells and normal human melanocytes. MITF mRNA and protein expressions were significantly inhibited in response to IFN-γ. IFN-γ inhibited CREB binding to the MITF promoter but did not affect CREB phosphorylation. Instead, IFN-γ inhibited the association of CBP and CREB through the increased association between CREB binding protein (CBP) and STAT1. These findings suggest that IFN-γ inhibits both basal and α-MSH-induced melanogenesis by inhibiting MITF expression. The inhibitory action of IFN-γ in α-MSH-induced melanogenesis is likely to be associated with the sequestration of CBP via the association between CBP and STAT1. These data suggest that IFN-γ plays a role in controlling inflammation- or UV-induced pigmentary changes.
authors.affiliation: Department of Pharmacology, Ajou University School of Medicine, Suwon, Korea; Chronic Inflammatory Disease Research Center, Ajou University School of Medicine, Suwon, Korea.
authors.forename: Jinyoung
authors.initials: J
authors.lastname: Son
authors.forename: Misun
authors.initials: M
authors.lastname: Kim
authors.forename: Ilo
authors.initials: I
authors.lastname: Jou
authors.forename: Kyoung Chan
authors.initials: KC
authors.lastname: Park
authors.forename: Hee Young
authors.initials: HY
authors.lastname: Kang
mesh_terms: D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D008543:Melanins / Q000096:biosynthesis*; D008544:Melanocytes / Q000187:drug effects / Q000201:enzymology; D008546:Melanoma, Experimental / Q000201:enzymology / Q000473:pathology; D008565:Membrane Proteins / Q000378:metabolism; D051739:Microphthalmia-Associated Transcription Factor / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding / Q000187:drug effects; D050794:STAT1 Transcription Factor / Q000378:metabolism; D000521:alpha-MSH / Q000494:pharmacology*
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D008543:Melanins; D008565:Membrane Proteins; D051739:Microphthalmia-Associated Transcription Factor; C498709:Pag1 protein, mouse; D010750:Phosphoproteins; D050794:STAT1 Transcription Factor; D000521:alpha-MSH; D007371:Interferon-gamma; D014442:Monophenol Monooxygenase
keywords: IFN-γ; melanogenesis; α-MSH