title: miR-181b promotes cell proliferation and reduces apoptosis by repressing the expression of adenylyl cyclase 9 (AC9) in cervical cancer cells.
abstract: MicroRNAs are a class of small, endogenous, non-coding RNAs that function as post-transcriptional regulators. In this study, we found that miR-181b promoted cell proliferation and inhibited cell apoptosis in cervical cancer cells. And we validated a new miR-181b target gene, adenylyl cyclase 9 (AC9). miR-181b restricted cAMP production by post-transcriptionally downregulating AC9 expression. Phenotypic experiments indicated that miR-181b and AC9 exerted opposite effects on cell proliferation and apoptosis.
authors.affiliation: Tianjin Life Science Research Center, Department of Microbiology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Tianjin Life Science Research Center, Department of Microbiology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Wang
authors.affiliation: Tianjin Life Science Research Center, Department of Microbiology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.
authors.forename: Shang
authors.initials: S
authors.lastname: Liu
authors.affiliation: Tianjin Life Science Research Center, Department of Microbiology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.
authors.forename: Pei-Pei
authors.initials: PP
authors.lastname: Zhang
authors.affiliation: Tianjin Life Science Research Center, Department of Microbiology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Tianjin Life Science Research Center, Department of Microbiology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: Tianjin Life Science Research Center, Department of Microbiology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China. Electronic address: htang2002@yahoo.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Tang
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000262:Adenylyl Cyclases / Q000235:genetics* / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000235:genetics; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; C540193:MIrn181 microRNA, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; C413923:adenylate cyclase 9
keywords: AC9; ASO; Adenylyl cyclase 9; Cervical cancer cell; EGFP; GAPDH; TUNEL; UTR; adenylyl cyclase 9; antisense oligonucleotide; cAMP; enhanced green fluorescence protein; glyceraldehyde-3-phosphate dehydrogenase; miR-181b; miRNA; microRNA; microRNA-181b; terminal deoxynucleotidyl transferase dUTP nick end labeling; untranslated region