title: Characterization of a mitochondrial manganese superoxide dismutase gene from Apis cerana cerana and its role in oxidative stress.
abstract: Mitochondrial manganese superoxide dismutase (mMnSOD) plays a vital role in the defense against reactive oxygen species (ROS) in eukaryotic mitochondria. In this study, we isolated and identified a mMnSOD gene from Apis cerana cerana, which we named AccSOD2. Several putative transcription factor-binding sites were identified within the 5'-flanking region of AccSOD2, which suggests that AccSOD2 may be involved in organismal development and/or environmental stress responses. Quantitative real-time PCR analysis showed that AccSOD2 is highly expressed in larva and pupae during different developmental stages. In addition, the expression of AccSOD2 could be induced by cold (4 °C), heat (42 °C), H2O2, ultraviolet light (UV), HgCl2, and pesticide treatment. Using a disc diffusion assay, we provide evidence that recombinant AccSOD2 protein can play a functional role in protecting cells from oxidative stress. Finally, the in vivo activities of AccSOD2 were measured under a variety of stressful conditions. Taken together, our results indicate that AccSOD2 plays an important role in cellular stress responses and anti-oxidative processes and that it may be of critical importance to honeybee survival.
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Haihong
authors.initials: H
authors.lastname: Jia
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong 271018, PR China; Yantai Research Institute, China Agricultural University, Yantai, Shandong 264670, PR China.
authors.forename: Rujiang
authors.initials: R
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Weina
authors.initials: W
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: lihan@sdau.edu.cn.
authors.forename: Han
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Xingqi
authors.initials: X
authors.lastname: Guo
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: bhxu@sdau.edu.cn.
authors.forename: Baohua
authors.initials: B
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects; D040461:Gene Components; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D014157:Transcription Factors; D013482:Superoxide Dismutase
keywords: Antioxidant activity; Apis cerana cerana; Mitochondrial manganese superoxide dismutase; Molecular characterization; Oxidative stress