title: Programmed assembly of polymer-DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition.
abstract: Soft micellar nanoparticles can be prepared from DNA conjugates designed to assemble via base pairing such that strands containing a polymer corona and a cholesterol tail generate controlled supramolecular architecture. Functionalization of one DNA conjugate strand with a biorecognition ligand results in shielding of the ligand when in the micelle, while encoding of the DNA sequences with overhangs allows supramolecular unpacking by addition of a complementary strand and sequence-specific unshielding of the ligand. The molecular assembly/disassembly and 'on-off' switch of the recognition signal is visualized by FRET pair signalling, PAGE and a facile turbidimetric binding assay, allowing direct and amplified readout of nucleic acid sequence recognition.
authors.affiliation: School of Pharmacy, University of Nottingham, University Park, Nottingham, NG7 2RD, UK. sebastian.spain@nottingham.ac.uk cameron.alexander@nottingham.ac.uk.
authors.forename: Johannes P
authors.initials: JP
authors.lastname: Magnusson
authors.forename: Francisco
authors.initials: F
authors.lastname: Fernández-Trillo
authors.forename: Giovanna
authors.initials: G
authors.lastname: Sicilia
authors.forename: Sebastian G
authors.initials: SG
authors.lastname: Spain
authors.forename: Cameron
authors.initials: C
authors.lastname: Alexander
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D008823:Micelles; D053758:Nanoparticles / Q000737:chemistry*; D009391:Nephelometry and Turbidimetry / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D008823:Micelles; D004247:DNA