title: Examining the effects of self-assembled monolayers on nanoporous gold based amperometric glucose biosensors.
abstract: Nanoporous gold (NPG) based biosensors have been constructed by covalently immobilizing glucose oxidase (GOx) onto self-assembled monolayers (SAMs). With p-benzoquinone (BQ) as a mediator, diffusion behavior and amperometric biosensor performance are evaluated by electrochemical characterization. The enzyme modified electrodes are demonstrated to show a thickness-sensitive behavior. Compared with planar polycrystalline gold, the unique porous structure of NPG has also been characterized via an electrochemical surface reconstruction process. Single-crystal gold-like electrochemical behavior on NPG and a comprehensive understanding of its impacts on sensor performance have been proposed.
authors.affiliation: Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials, Ministry of Education, School of Materials Science and Engineering, Shandong University, Jinan 250061, People's Republic of China. pcsi@sdu.edu.cn.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Xiao
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Meng'en
authors.initials: M
authors.lastname: Wang
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.forename: Pengchao
authors.initials: P
authors.lastname: Si
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D016227:Benzoquinones / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004058:Diffusion; D004563:Electrochemistry; D004566:Electrodes; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006801:Humans; D058608:Nanopores
chemical_list: D016227:Benzoquinones; C004532:quinone; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose