title: In situ mixed donor synthesis of ampicillin with ethylene glycol co-solvent.
abstract: Ethylene glycol has the ability to act as a reactant when employed as a co-solvent for the enzymatic synthesis of ampicillin from (R)-phenylglycine methyl ester and 6-aminopenicillanic acid. The side reaction positively affects yield because its product, (R)-phenylglycine hydroxyethyl ester, is an intermediate for ampicillin synthesis.
authors.affiliation: Parker H. Petit Institute of Bioengineering and Biosciences, School of Chemical and Biomolecular Engineering, Atlanta, Georgia.
authors.forename: Andria L
authors.initials: AL
authors.lastname: Deaguero
authors.forename: Andreas S
authors.initials: AS
authors.lastname: Bommarius
mesh_terms: D000667:Ampicillin / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D001709:Biotechnology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D004951:Esterification; D019855:Ethylene Glycol / Q000737:chemistry* / Q000378:metabolism; D010399:Penicillin Amidase / Q000235:genetics / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D000667:Ampicillin; D010399:Penicillin Amidase; D019855:Ethylene Glycol
keywords: ampicillin; co-solvent; transesterification; β-lactam antibiotics