title: Influence of the excision shock on the protein metabolism ofVicia faba L. meristematic root cells.
abstract: UsingVicia faba root meristems we have shown that protein synthesis was dramatically changed after excision. The amino-acid incorporation dropped to 13% of the level in the unexcised control. This downshift was a direct consequence of the breakdown of polysomes which are converted into monosomes. In order to perform an analysis of the protein pattern by two-dimensional gel electrophoresis, endogenous proteolytic activity, which is high in broad bean root, had to be inhibited. Therefore, several protease inhibitors were tested and a very efficient inhibitor pool was obtained which could be used during the preparation of meristematic cell extracts. Protein-pattern analysis showed important differences between the unexcised control and excised apices. The number of proteins synthesized after excision droped from 250 in the control to 80, as a consequence of polysome breakdown. Futhermore, we present evidence that new and apparently specific proteins are synthesized in response to this excision shock.
authors.affiliation: Laboratoire de Physiologie Cellulaire Végétale, Université Paris 7, 2 place Jussieu, F-75251, Paris Cedex 05, France.
authors.forename: C
authors.initials: C
authors.lastname: Theillet
authors.forename: F
authors.initials: F
authors.lastname: Delpeyroux
authors.forename: M
authors.initials: M
authors.lastname: Fiszman
authors.forename: P
authors.initials: P
authors.lastname: Reigner
authors.forename: R
authors.initials: R
authors.lastname: Esnault