title: Localization of arabinogalactan protein 6 fused with Sirius ultramarine fluorescent protein in Arabidopsis pollen and pollen tubes.
abstract: Following studies on the transcriptome of pollen tubes of an agp6 agp11 Arabidopsis double null mutant, together with the knowledge that arabinogalactan protein (AGP) 6 is important for male gametogenesis and pollen germination, we sought to know whether AGP6 could be present in the vegetative cell wall or in the generative cell wall or in both. To that end, Arabidopsis plants were transformed with AGP6 gene sequence fused with Sirius fluorescent protein. Fluorescent blue light emission could be detected in the vegetative cell wall only. This result supports the model by which AGP6 and other pollen AGPs are probably important signaling molecules at the pollen tube apex.
authors.affiliation: Departamento de Biologia; Faculdade de Ciências; Universidade do Porto; Porto, Portugal; BioFIG; Center for Biodiversity; Functional and Integrative Genomics; Porto, Portugal.
authors.forename: Luís Gustavo
authors.initials: LG
authors.lastname: Pereira
authors.forename: Mário
authors.initials: M
authors.lastname: Costa
authors.forename: Sílvia
authors.initials: S
authors.lastname: Coimbra
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D009088:Mucoproteins / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D011058:Pollen / Q000378:metabolism*; D053205:Pollen Tube / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D009088:Mucoproteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C006619:arabinogalactan proteins
keywords: AGP6; Arabidopsis; Sirius fluorescent protein; arabinogalactan proteins; pollen tube