title: Highly sensitive detection of cancer antigen 15-3 using novel avian IgY antibodies.
abstract: Early detection of cancer development is crucial for successful therapy and for monitoring patient outcome. Various immunodiagnostic methods are able to detect pathological changes in the human body ahead of symptomatic manifestation of the disease. Most immunological examinations are based on the detection of specific tumor markers in body fluids. Of the various cancer-specific proteins used for breast cancer diagnostics, one of the most commonly applied is the cancer antigen 15-3 (CA 15-3). An elevation in its serum level (>25-40 U/ml) usually correlates with tumor malignancy. The CA 15-3 antigen is also used for monitoring patients after surgical treatment and for measuring therapeutic efficacy. Herein, we present the generation of polyclonal IgY antibodies isolated from egg yolks of immunized hens and their application for CA 15-3 detection. The developed sandwich ELISA assay showed a detection limit of 0.028 U/ml, thus demonstrating its potential for clinical applications.
authors.affiliation: Wroclaw University of Technology, Faculty of Chemistry, Division of Medicinal Chemistry and Microbiology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Grzywa
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Łupicka-Słowik
authors.forename: Maciej
authors.initials: M
authors.lastname: Walczak
authors.forename: Magdalena
authors.initials: M
authors.lastname: Idzi
authors.forename: Kamila
authors.initials: K
authors.lastname: Bobrek
authors.forename: Stephane
authors.initials: S
authors.lastname: Boivin
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gaweł
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Stefaniak
authors.forename: Józef
authors.initials: J
authors.lastname: Oleksyszyn
authors.forename: Marcin
authors.initials: M
authors.lastname: Sieńczyk
mesh_terms: D000818:Animals; D000915:Antibody Affinity; D002645:Chickens; D004530:Egg Yolk; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes; D005260:Female; D006801:Humans; D007074:Immunoglobulin G; D007136:Immunoglobulins / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D018396:Mucin-1 / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000939:Epitopes; C016512:IgY; D007074:Immunoglobulin G; D007136:Immunoglobulins; D018396:Mucin-1