title: A novel pH-controlled immunosensor using hollow mesoporous silica and apoferritin combined system for target virus assay.
abstract: A novel pH-controlled immunosensor using hollow mesoporous silica and apoferritin combined system has been reported for the first time. The goal of this study was to introduce more electroactive probes into the electrochemical immunosensor. Under such background, we focused our attention on hollow mesoporous silica and apoferritin, both of which have admirable accommodating performances and can be applied for encapsulating electroactive probes. Based on the pH-controlled disassociation-reconstitution process of apoferritin and the mesoporous channels of silica, after the appropriate chemical modification, apoferritin could be fabricated with silica. The results showed that the hollow mesoporous silica and apoferritin combined system was successfully assembled. The excellent performance of the combined system can be applied for ultrasensitive detection of a target virus.
authors.affiliation: College of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong 271018, China.
authors.forename: Xindong
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong 271018, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Dong
authors.affiliation: College of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong 271018, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong 271018, China.
authors.forename: Yanzhen
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong 271018, China. Electronic address: ashy@sdau.edu.cn.
authors.forename: Shiyun
authors.initials: S
authors.lastname: Ai
mesh_terms: D000818:Animals; D001052:Apoferritins / Q000737:chemistry*; D001353:Avian Leukosis / Q000175:diagnosis; D001354:Avian Leukosis Virus / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000295:instrumentation*; D001717:Birds / Q000821:virology; D055664:Electrochemical Techniques / Q000295:instrumentation*; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000295:instrumentation*; D057230:Limit of Detection; D016062:Porosity; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D012822:Silicon Dioxide; D001052:Apoferritins
keywords: Apoferritin; Electrochemical immunosensor; Hollow mesoporous silica; Ultrasensitive detection