title: Determination of toxicity of neonicotinoids on the genome level using chemogenomics in yeast.
abstract: Neonicotinoid insecticides are an important contribution to plant protection products. At the same time, their environmental impact on non-target organisms is often problematic. It has been shown that the toxicity of formulations of neonicotinoid insecticides can originate from non-neonicotinoid additives. In the present study we used chemogenomics to analyse side effects of purified neonicotinoids, additives and formulations on the genome-wide scale. We show that the additives in formulations have more pronounced effects than the active components, and that these effects could explain previously observed negative effects of neonicotinoid insecticides on spermatogenesis in animals. We also demonstrate that cell wall organization and biogenesis in yeast is negatively affected by neonicotinoid substances.
authors.affiliation: Department of Molecular and Biomedical Sciences, Jozef Stefan Institute, Jamova 39, SI-1000 Ljubljana, Slovenia.
authors.forename: Mojca
authors.initials: M
authors.lastname: Mattiazzi Ušaj
authors.affiliation: Department of Molecular and Biomedical Sciences, Jozef Stefan Institute, Jamova 39, SI-1000 Ljubljana, Slovenia.
authors.forename: Petra
authors.initials: P
authors.lastname: Kaferle
authors.affiliation: Department of Molecular and Biomedical Sciences, Jozef Stefan Institute, Jamova 39, SI-1000 Ljubljana, Slovenia; Laboratory for Environmental Research, University of Nova Gorica, Vipavska 13, SI-5000 Nova Gorica, Slovenia.
authors.forename: Alenka
authors.initials: A
authors.lastname: Toplak
authors.affiliation: Laboratory for Environmental Research, University of Nova Gorica, Vipavska 13, SI-5000 Nova Gorica, Slovenia. Electronic address: polonca.trebse@zf.uni-lj.si.
authors.forename: Polonca
authors.initials: P
authors.lastname: Trebše
authors.affiliation: Department of Molecular and Biomedical Sciences, Jozef Stefan Institute, Jamova 39, SI-1000 Ljubljana, Slovenia. Electronic address: uros.petrovic@ijs.si.
authors.forename: Uroš
authors.initials: U
authors.lastname: Petrovič
mesh_terms: D016681:Genome, Fungal / Q000187:drug effects*; D007306:Insecticides / Q000737:chemistry / Q000633:toxicity*; D056186:Metagenomics / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects* / Q000235:genetics*
chemical_list: D007306:Insecticides
keywords: Additives; Chemogenomics; Neonicotinoids; Side effects; Spermatogenesis; Yeast Saccharomyces cerevisiae