title: Proteomic profiling reveals upregulated protein expression of hsp70 in keloids.
abstract: BACKGROUND
  The biochemical characteristics of keloid-derived fibroblasts differ from those of adjacent normal fibroblasts, and these differences are thought to be the cause of abnormal fibrosis. Therefore, we investigated the characteristic proteins that are differentially expressed in keloid-derived fibroblasts using proteomics tools.
  
  
  OBJECTIVE
  We attempted to investigate the novel proteins that play important roles in the pathophysiology of keloids.
  
  
  METHODS
  Proteomics analysis was performed to identify differentially expressed proteins in keloid-derived fibroblasts. Keloid-derived fibroblasts and adjacent normal fibroblasts were analyzed with 2-DAGE. We validated these proteins with immunoblot analysis, real-time RT-PCR, and immunohistochemistry.
  
  
  RESULTS
  Sixteen differentially expressed protein spots were identified in keloid-derived fibroblasts. Among them, heat shock protein 70 (Hsp70) was specifically upregulated in keloid-derived fibroblasts. Also, immunohistochemistry and western blot analysis revealed increased Hsp70, TGF- β , and PCNA expressions in keloids compared to normal tissue.
  
  
  CONCLUSIONS
  Hsp70 is overexpressed in keloid fibroblasts and tissue. The overexpression of Hsp70 may be involved in the pathogenesis of keloids, and the inhibition of Hsp70 could be a new therapeutic tool for the treatment of keloids.
authors.affiliation: Department of Dermatology and Cutaneous Biology Research Institute, Yonsei University College of Medicine, 50 Yonseiro, Seodaemun-gu, Seoul 120-752, Republic of Korea.
authors.forename: Ju Hee
authors.initials: JH
authors.lastname: Lee
authors.forename: Jung U
authors.initials: JU
authors.lastname: Shin
authors.forename: Inhee
authors.initials: I
authors.lastname: Jung
authors.forename: Hemin
authors.initials: H
authors.lastname: Lee
authors.forename: Dong Kyun
authors.initials: DK
authors.lastname: Rah
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Jung
authors.forename: Won Jai
authors.initials: WJ
authors.lastname: Lee
mesh_terms: D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D020869:Gene Expression Profiling; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D007627:Keloid / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D040901:Proteomics; D016212:Transforming Growth Factor beta / Q000096:biosynthesis; D015854:Up-Regulation
chemical_list: D018840:HSP70 Heat-Shock Proteins; D018809:Proliferating Cell Nuclear Antigen; D016212:Transforming Growth Factor beta