title: A facile, sensitive and selective fluorescent probe for heparin based on aggregation-induced emission.
abstract: A facile, rapidly responsive fluorescence turn-on probe for heparin with high selectivity and sensitivity was reported in this paper. The probe could aggregate on the negatively charged heparin template through electrostatic interactions and then display intense fluorescence due to its aggregation-induced emission (AIE) characteristics. Under optimal condition, the probe showed high selectivity to heparin over chondroitin sulfate(ChS), hyaluronic acid (HA), dextran (DeX) and other substances, with a linear range of 0.2-14 μg/mL, and a detection limit of 57.6 ng/mL. In diluted serum, it also showed good performance.
authors.affiliation: Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology(Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, PR China.
authors.forename: Hualong
authors.initials: H
authors.lastname: Liu
authors.forename: Panshu
authors.initials: P
authors.lastname: Song
authors.forename: Ruirui
authors.initials: R
authors.lastname: Wei
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.forename: Aijun
authors.initials: A
authors.lastname: Tong
mesh_terms: D002809:Chondroitin Sulfates / Q000032:analysis / Q000737:chemistry; D003911:Dextrans / Q000032:analysis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006493:Heparin / Q000032:analysis* / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000032:analysis / Q000737:chemistry; D057230:Limit of Detection; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
chemical_list: D003911:Dextrans; D005456:Fluorescent Dyes; D006820:Hyaluronic Acid; D006493:Heparin; D002809:Chondroitin Sulfates
keywords: Aggregation-induced emission; Electrostatic interaction; Heparin; Positively charged salicylaldehyde azine