title: Identification and characterization of DNA clones encoding group-II glycinin subunits.
abstract: DNA clones that encode the group-II subunits of soybean glycinin were identified and compared with clones for group-I subunits. The group-I clones hybridize weakly to those from group-II at low stringency, but fail to hybridize with them at moderate or high stringency. The genes for the group-II subunits are contained in 13 and 9 kb EcoRI fragments of genomic DNA in cultivar CX635-1-1-1. These fragments contain genes for subunits A5A4B3 and A3B4, respectively. The larger size of mature group-II subunits compared with group-I subunits is correlated with a larger sized mRNA. However, the gross arrangement of introns and exons within the group-II coding regions appears to be the same as for the genes which encode group-I subunits. Messenger RNA for both groups of glycinin subunits appear in the seed at the same developmental interval, and their appearance lags slightly behind that of mRNAs for the a/a' subunits of β-conglycinin. These data indicate that the glycinin gene family is more complex than previously thought.
authors.affiliation: USDA and the Department of Agronomy, Purdue University, 47907, West Lafayette, IN, USA.
authors.forename: B
authors.initials: B
authors.lastname: Scallon
authors.forename: V H
authors.initials: VH
authors.lastname: Thanh
authors.forename: L A
authors.initials: LA
authors.lastname: Floener
authors.forename: N C
authors.initials: NC
authors.lastname: Nielsen