title: Investigations on the interactions of λphage-derived peptides against the SrtA mechanism in Bacillus anthracis.
abstract: Bacillus anthracis is a well-known bioweapon pathogen, which coordinates the expression of its virulence factors in response to a specific environmental signal by its protein architecture. Absences of sortase signal functioning may fail to assemble the surface linked proteins and so B. anthracis cannot sustain an infection with host cells. Targeting the signaling mechanism of B. anthracis can be achieved by inhibition of SrtA enzyme through λphage-derived plyG. The lysin enzyme plyG is experimentally proven as bacteriolytic agent, specifically kill's B. anthracis by inhibiting the SrtA. Here, we have screened the peptides from λphage lysin, and these peptides are having the ability as LPXTG competitive inhibitors. In comparison to the activator peptide LPXTG binding motif, λphage lysin based inhibitor peptides are having much supremacy towards binding of SrtA. Finally, peptide structures extracted from PlyG are free from toxic, allergic abilities and also have the ability to terminate the signal transduction mechanism in B. anthracis.
authors.affiliation: Computer-Aided Drug Design and Molecular Modeling Lab, Department of Bioinformatics, Alagappa University, Karaikudi, 630004, Tamil Nadu, India.
authors.forename: Chandrabose
authors.initials: C
authors.lastname: Selvaraj
authors.forename: Poonam
authors.initials: P
authors.lastname: Singh
authors.forename: Sanjeev Kumar
authors.initials: SK
authors.lastname: Singh
mesh_terms: D019881:Aminoacyltransferases / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001408:Bacillus anthracis / Q000187:drug effects*; D001426:Bacterial Proteins / Q000378:metabolism*; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D010455:Peptides; D014764:Viral Proteins; D019881:Aminoacyltransferases; C120542:sortase A; C465450:PlyG lysin, gamma phage; D003546:Cysteine Endopeptidases; D009238:N-Acetylmuramoyl-L-alanine Amidase