title: MYB10 plays a major role in the regulation of flavonoid/phenylpropanoid metabolism during ripening of Fragaria x ananassa fruits.
abstract: This work characterized the role of the R2R3-MYB10 transcription factor (TF) in strawberry fruit ripening. The expression of this TF takes place mainly in the fruit receptacle and is repressed by auxins and activated by abscisic acid (ABA), in parallel to the ripening process. Anthocyanin was not produced when FaMYB10 expression was transiently silenced in fruit receptacles. An increase in FaMYB10 expression was observed in water-stressed fruits, which was accompanied by an increase in both ABA and anthocyanin content. High-throughput transcriptomic analyses performed in fruits with downregulated FaMYB10 expression indicated that this TF regulates the expression of most of the Early-regulated Biosynthesis Genes (EBGs) and the Late-regulated Biosynthesis Genes (LBGs) genes involved in anthocyanin production in ripened fruit receptacles. Besides, the expression of FaMYB10 was not regulated by FaMYB1 and vice versa. Taken together, all these data clearly indicate that the Fragaria × ananassa MYB10 TF plays a general regulatory role in the flavonoid/phenylpropanoid pathway during the ripening of strawberry.
authors.affiliation: Departamento de Bioquímica y Biología Molecular. Edificio Severo Ochoa, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario CEIA3, Universidad de Córdoba, 14071 Córdoba, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Medina-Puche
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Cumplido-Laso
authors.forename: Francisco
authors.initials: F
authors.lastname: Amil-Ruiz
authors.forename: Thomas
authors.initials: T
authors.lastname: Hoffmann
authors.forename: Ludwig
authors.initials: L
authors.lastname: Ring
authors.forename: Antonio
authors.initials: A
authors.lastname: Rodríguez-Franco
authors.forename: José Luis
authors.initials: JL
authors.lastname: Caballero
authors.forename: Wilfried
authors.initials: W
authors.lastname: Schwab
authors.forename: Juan
authors.initials: J
authors.lastname: Muñoz-Blanco
authors.forename: Rosario
authors.initials: R
authors.lastname: Blanco-Portales
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000872:Anthocyanins / Q000378:metabolism; D003433:Crosses, Genetic; D005419:Flavonoids / Q000378:metabolism*; D031985:Fragaria / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005638:Fruit / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D020868:Gene Silencing; D007210:Indoleacetic Acids / Q000494:pharmacology; D055432:Metabolomics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D020005:Propanols / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D005419:Flavonoids; D007210:Indoleacetic Acids; D010940:Plant Proteins; D020005:Propanols; D014157:Transcription Factors; C439395:1-phenylpropanol; D000040:Abscisic Acid
keywords: Anthocyanins; flavonoid/phenylpropanoid biosynthesis; fruit receptacle; ripening; strawberry; transcription factor.