title: Specific plasma autoantibody reactivity in myelodysplastic syndromes.
abstract: Increased autoantibody reactivity in plasma from Myelodysplastic Syndromes (MDS) patients may provide novel disease signatures, and possible early detection. In a two-stage study we investigated Immunoglobulin G reactivity in plasma from MDS, Acute Myeloid Leukemia post MDS patients, and a healthy cohort. In exploratory Stage I we utilized high-throughput protein arrays to identify 35 high-interest proteins showing increased reactivity in patient subgroups compared to healthy controls. In validation Stage II we designed new arrays focusing on 25 of the proteins identified in Stage I and expanded the initial cohort. We validated increased antibody reactivity against AKT3, FCGR3A and ARL8B in patients, which enabled sample classification into stable MDS and healthy individuals. We also detected elevated AKT3 protein levels in MDS patient plasma. The discovery of increased specific autoantibody reactivity in MDS patients, provides molecular signatures for classification, supplementing existing risk categorizations, and may enhance diagnostic and prognostic capabilities for MDS.
authors.affiliation: 1] Department of Genetics, Stanford University School of Medicine, Stanford, California, USA [2].
authors.forename: George I
authors.initials: GI
authors.lastname: Mias
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Kunju
authors.initials: K
authors.lastname: Sridhar
authors.forename: Donald
authors.initials: D
authors.lastname: Sharon
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
authors.forename: Hogune
authors.initials: H
authors.lastname: Im
authors.forename: Michael P
authors.initials: MP
authors.lastname: Snyder
authors.forename: Peter L
authors.initials: PL
authors.lastname: Greenberg
mesh_terms: D020727:ADP-Ribosylation Factors / Q000097:blood / Q000276:immunology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D015331:Cohort Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000145:classification / Q000175:diagnosis* / Q000276:immunology; D011379:Prognosis; D040081:Protein Array Analysis; D051057:Proto-Oncogene Proteins c-akt / Q000097:blood / Q000276:immunology; D017452:Receptors, IgG / Q000097:blood / Q000276:immunology; D012189:Retrospective Studies; D012306:Risk
chemical_list: C511801:ARL8B protein, human; D001323:Autoantibodies; C496124:FCGR3A protein, human; D017452:Receptors, IgG; C491754:AKT3 protein, human; D051057:Proto-Oncogene Proteins c-akt; D020727:ADP-Ribosylation Factors