title: Cells isolated from human periapical cysts express mesenchymal stem cell-like properties.
abstract: We provide a detailed description of mesenchymal stem cells (MSCs) isolated from human periapical cysts, which we have termed hPCy-MSCs. These cells have a fibroblast-like shape and adhere to tissue culture plastic surfaces. hPCy-MSCs possess high proliferative potential and self-renewal capacity properties. We characterised the immunophenotype of hPCy-MSCs (CD73(+), CD90(+), CD105(+), CD13(+), CD29(+), CD44(+), CD45(-), STRO-1(+), CD146(+)) by flow cytometry and immunofluorescence. hPCy-MSCs possess the potential to differentiate into osteoblast- and adipocyte-like cells in vitro. Multi-potentiality was evaluated with culture-specific staining and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis for osteo/odontogenic and adipogenic markers. This is the first report to indicate that human periapical cysts contain cells with MSC-like properties. Taken together, our findings indicate that human periapical cysts could be a rich source of MSCs.
authors.affiliation: 1. Unit of Maxillofacial Surgery, Calabrodental, Crotone, Italy; ; 2. Tecnologica Research Institute, Biomedical Section, Crotone, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Marrelli
authors.forename: Francesco
authors.initials: F
authors.lastname: Paduano
authors.forename: Marco
authors.initials: M
authors.lastname: Tatullo
mesh_terms: D000328:Adult; D001483:Base Sequence; D002454:Cell Differentiation; D019070:Cell Lineage; D017931:DNA Primers; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology; D018613:Microscopy, Confocal; D011842:Radicular Cyst / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction; D013234:Stem Cells; D055815:Young Adult
chemical_list: D017931:DNA Primers
keywords: Mesenchymal stem cells (MSCs); adipogenic differentiation; cyst; mesenchymal stem cells isolated from human periapical cyst (hPCy-MSCs); odontogenic cyst.; osteogenic differentiation; periapical inflammatory tissues