title: Infection of cowpea mesophyll protoplasts with cowpea chlorotic mottle virus (CCMV) RNA encapsulated in large liposomes.
abstract: It has been demonstrated that cowpea chlorotic mottle virus RNA encapsulated in phosphatidyl serine/cholesterol reverse evaporation vesicles (REV) could infect cowpea mesophyll protoplasts under conditions known to enhance liposome-protoplast interactions. Positively charged phosphatidylcholine/stearylamine multilamellar liposomes did not deliver functional CCMV RNA despite their very high nucleic acid trapping capacity and their high affinity for protoplasts.
authors.affiliation: Program in Genetics and Cell Biology, Molecular Genetics, Heald Hall, Washington State University, 99164-4350, Pullman, WA, USA.
authors.forename: A A
authors.initials: AA
authors.lastname: Christen
authors.forename: P F
authors.initials: PF
authors.lastname: Lurquin