title: [Carvacrol pretreatment attenuates myocardial oxidative stress and apoptosis following myocardial ischemia-reperfusion in mice].
abstract: OBJECTIVE
  To investigate the effect of carvacrol pretreatment on myocardial ischemia-reperfusion (I/R) injury and its underlying mechanisms.
  
  
  METHODS
  Wild-type male C57 BL/6 mice were randomized into 5 groups (n=13), namely the sham-operated group, vehicle (DMSO in saline)+ I/R group, carvacrol (20 mg/kg) + I/R group, carvacrol (40 mg/kg) + I/R group, and carvacrol (60 mg/kg) + I/R group. The mouse models of myocardial I/R injury were established by a 45-min occlusion of the left anterior descending coronary artery (LAD) followed by reperfusion for 2 h. Carvacrol or vehicle was administered intravenously 15 min before LAD occlusion. After reperfusion, the mice were examined for myocardial oxidative stress level and apoptosis rate.
  
  
  RESULTS
  Compared with the vehicle group, the 3 carvacrol-pretreated groups showed significantly reduced myocardial infarct size, oxidative stress level and cardiac myocyte apoptosis rate (P<0.01).
  
  
  CONCLUSIONS
  Carvacrol can protect against myocardial I/R injury by inhibiting myocardial oxidative stress and apoptosis in mice.
authors.affiliation: Electrophysiological and pacemaker treatment center, Department of Cardiology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.E-mail: songxudong@msn.cn.
authors.forename: Xudong
authors.initials: X
authors.lastname: Song
authors.forename: Aihua
authors.initials: A
authors.lastname: Chen
authors.forename: Yingfeng
authors.initials: Y
authors.lastname: Liu
authors.forename: Xian-Bao
authors.initials: XB
authors.lastname: Wang
authors.forename: Yijun
authors.initials: Y
authors.lastname: Zhou
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Xiuli
authors.initials: X
authors.lastname: Zhang
authors.forename: Lizi
authors.initials: L
authors.lastname: Wang
authors.forename: Pingzhen
authors.initials: P
authors.lastname: Yang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002316:Cardiotonic Agents / Q000494:pharmacology; D000081007:Cymenes; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D039821:Monoterpenes / Q000494:pharmacology*; D009203:Myocardial Infarction / Q000473:pathology; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D011897:Random Allocation
chemical_list: D000975:Antioxidants; D002316:Cardiotonic Agents; D000081007:Cymenes; D039821:Monoterpenes; C073316:carvacrol