title: Induction of high mitotic index in Petunia suspension cultures by sequential treatment with aphidicolin and colchicine.
abstract: Significantly higher than normal mitotic index (MI) values were induced in Petunia cell suspensions following treatments with colchicine, aphidicolin, drastic medium replacement, or a sequential application of aphidicolin and colchicine. This last treatment yielded the highest MI values: cells incubated with 30 μg/ml aphidicolin for 18 h, then cultured in drug-free medium for 8 h and finally exposed to 0.1% colchicine for 8 additional hours exhibited MI of 62.8% and 65.7% respectively, for the two cell lines in study.
authors.affiliation: Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, 50 250, Bet Dagan, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Guri
authors.forename: A
authors.initials: A
authors.lastname: Zelcer
authors.forename: S
authors.initials: S
authors.lastname: Izhar