title: Highly aligned porous Ti scaffold coated with bone morphogenetic protein-loaded silica/chitosan hybrid for enhanced bone regeneration.
abstract: Porous Ti has been widely investigated for orthopedic and dental applications on account of their ability to promote implant fixation via bone ingrowth into pores. In this study, highly aligned porous Ti scaffolds coated with a bone morphogenetic protein (BMP)-loaded silica/chitosan hybrid were produced, and their bone regeneration ability was evaluated by in vivo animal experiments. Reverse freeze casting allowed for the creation of highly aligned pores, resulting in a high compressive strength of 254 ± 21 MPa of the scaffolds at a porosity level of ∼51 vol %. In addition, a BMP-loaded silica/chitosan hybrid coating layer with a thickness of ∼1 μm was uniformly deposited on the porous Ti scaffold, which enabled the sustained release of the BMP over a prolonged period of time up to 26 days. The cumulative amount of the BMP released was ∼4 μg, which was much higher than that released from the specimen without a hybrid coating layer. In addition, the bone regeneration ability of the porous Ti scaffold with a BMP-loaded silica/chitosan coating layer was examined by in vivo animal testing using a rabbit calvarial defect model and compared with those of the as-produced porous Ti scaffold and porous Ti scaffold with a silica/chitosan coating layer. After 4 weeks of healing, the specimen coated with a BMP-loaded silica/chitosan hybrid showed a much higher bone regeneration volume (∼36%) than the as-produced specimen (∼15%) (p < 0.005) and even the specimen coated with a silica/chitosan hybrid (∼25%) (p < 0.05).
authors.affiliation: WCU Hybrid Materials Program, Department of Materials Science and Engineering, Seoul National University, Seoul, 151-742, Korea.
authors.forename: Hyun-Do
authors.initials: HD
authors.lastname: Jung
authors.forename: Se-Won
authors.initials: SW
authors.lastname: Yook
authors.forename: Cheol-Min
authors.initials: CM
authors.lastname: Han
authors.forename: Tae-Sik
authors.initials: TS
authors.lastname: Jang
authors.forename: Hyoun-Ee
authors.initials: HE
authors.lastname: Kim
authors.forename: Young-Hag
authors.initials: YH
authors.lastname: Koh
authors.forename: Yuri
authors.initials: Y
authors.lastname: Estrin
mesh_terms: D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000737:chemistry / Q000494:pharmacology; D001861:Bone Regeneration / Q000187:drug effects*; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing / Q000379:methods; D016062:Porosity; D011817:Rabbits; D012822:Silicon Dioxide / Q000737:chemistry / Q000494:pharmacology; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology
chemical_list: D019485:Bone Morphogenetic Proteins; D020099:Coated Materials, Biocompatible; D012822:Silicon Dioxide; D048271:Chitosan; D014025:Titanium
keywords: BMP-2; aligned pores; bone regeneration; hybrid coating; titanium