title: Bioassay-guided isolation of a sesquiterpene lactone of deoxyelephantopin from Elephantopus scaber Linn. active on Trypanosome brucei rhodesience.
abstract: Methanolic extracts of 70 Malaysia plants were screened for their in vitro antitrypanosomal activity using Trypanosome brucei rhodesience, strain STIB 900 and mouse skeletal cell (L-6) in cytotoxicity activity assay. Results indicated that methanol extract from Elephantopus scaber Linn. (E. scaber) possessed the highest value of antitrypanosomal activity with good selectivity index (antitrypanosomal IC50 of 0.22±0.02 μg/ml, SI value of 204.55). Based on these results, E. scaber was chosen for further study by applying bioassay guided fractionation to isolate its antiprotozoal principle. The antiprotozoal principle was isolated from the ethyl acetate partition through solvent fractionation and crystallization process. The isolated active compound 1 was identified as deoxyelephantopin on the basis of its spectral analysis (FTIR, MS, 1D and 2D NMR).
authors.affiliation: Malaysian Institute of Pharmaceuticals and Nutraceuticals, Ministry of Science, Technology and Innovation, Malaysia.
authors.forename: Zuriati
authors.initials: Z
authors.lastname: Zahari
authors.affiliation: Universiti Teknologi MARA Negeri Sembilan, Kampus Kuala Pilah, Beting, 72000 Kuala Pilah, Negeri Sembilan, Malaysia.
authors.forename: Nor Akmalazura
authors.initials: NA
authors.lastname: Jani
authors.affiliation: Malaysian Institute of Pharmaceuticals and Nutraceuticals, Ministry of Science, Technology and Innovation, Malaysia.
authors.forename: Azimah
authors.initials: A
authors.lastname: Amanah
authors.affiliation: Malaysian Institute of Pharmaceuticals and Nutraceuticals, Ministry of Science, Technology and Innovation, Malaysia.
authors.forename: Mohd Naffidi Abdul
authors.initials: MN
authors.lastname: Latif
authors.affiliation: Universiti Sains Malaysia, Penang, Malaysia.
authors.forename: Mohamed Isa Abdul
authors.initials: MI
authors.lastname: Majid
authors.affiliation: Malaysian Institute of Pharmaceuticals and Nutraceuticals, Ministry of Science, Technology and Innovation, Malaysia; Forest Research Institute Malaysia, 52109 Kepong, Selangor, Malaysia. Electronic address: ilhamipharm@gmail.com.
authors.forename: Mohd Ilham
authors.initials: MI
authors.lastname: Adenan
mesh_terms: D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D007783:Lactones / Q000032:analysis / Q000494:pharmacology*; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012717:Sesquiterpenes / Q000032:analysis / Q000494:pharmacology*; D014344:Trypanocidal Agents / Q000494:pharmacology*; D016833:Trypanosoma brucei rhodesiense / Q000187:drug effects*; D014353:Trypanosomiasis, African / Q000469:parasitology*
chemical_list: D007783:Lactones; D010936:Plant Extracts; D012717:Sesquiterpenes; D014344:Trypanocidal Agents; C528427:deoxyelephantopin
keywords: Bioassay-guided isolation ;; Deoxyelephantopin; Elephantopus scaber; Trypanosome brucei rhodesience; Trypanosomiasis