title: Diversification of fungal specific class a glutathione transferases in saprotrophic fungi.
abstract: Glutathione transferases (GSTs) form a superfamily of multifunctional proteins with essential roles in cellular detoxification processes and endogenous metabolism. The distribution of fungal-specific class A GSTs was investigated in saprotrophic fungi revealing a recent diversification within this class. Biochemical characterization of eight GSTFuA isoforms from Phanerochaete chrysosporium and Coprinus cinereus demonstrated functional diversity in saprotrophic fungi. The three-dimensional structures of three P. chrysosporium isoforms feature structural differences explaining the functional diversity of these enzymes. Competition experiments between fluorescent probes, and various molecules, showed that these GSTs function as ligandins with various small aromatic compounds, derived from lignin degradation or not, at a L-site overlapping the glutathione binding pocket. By combining genomic data with structural and biochemical determinations, we propose that this class of GST has evolved in response to environmental constraints induced by wood chemistry.
authors.affiliation: Université de Lorraine, IAM, UMR 1136, IFR 110 EFABA, Vandoeuvre-les-Nancy, France ; INRA, IAM, UMR 1136, Champenoux, France ; Laboratoire de biotechnologie, Pôle Biotechnologie et Sylviculture Avancée, FCBA, Campus Forêt-Bois de Pierroton, Cestas, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Mathieu
authors.forename: Pascalita
authors.initials: P
authors.lastname: Prosper
authors.forename: Frédérique
authors.initials: F
authors.lastname: Favier
authors.forename: Luc
authors.initials: L
authors.lastname: Harvengt
authors.forename: Claude
authors.initials: C
authors.lastname: Didierjean
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Jacquot
authors.forename: Mélanie
authors.initials: M
authors.lastname: Morel-Rouhier
authors.forename: Eric
authors.initials: E
authors.lastname: Gelhaye
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003302:Coprinus / Q000201:enzymology*; D003460:Crystallization; D017931:DNA Primers; D005982:Glutathione Transferase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D020075:Phanerochaete / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D013050:Spectrometry, Fluorescence
chemical_list: D017931:DNA Primers; D005982:Glutathione Transferase