title: Production of fat-1 transgenic rats using a post-natal female germline stem cell line.
abstract: Germline stem cell lines possess the abilities of self-renewal and differentiation, and have been established from both mouse and human ovaries. Here, we established a new female germline stem cell (FGSC) line from post-natal rats by immunomagnetic sorting for Fragilis, which showed a normal karyotype, high telomerase activity, and a consistent gene expression pattern of primordial germ cells after 1 year of culture. Using an in vitro differentiation system, the FGSC line could differentiate into oocytes. After liposome-based transfection with green fluorescent protein (GFP) or fat-1 vectors, the FGSCs were transplanted into the ovaries of infertile rats. The transplanted FGSCs underwent oogenesis, and the rats produced offspring carrying the GFP or fat-1 transgene after mating with wild-type male rats. The efficiency of gene transfer was 27.86-28.00%, and 2 months was needed to produce transgenic rats. These findings have implications in biomedical research and potential applications in biotechnology.
authors.affiliation: Key Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, No. 800. Dongchuan Road, Minhang District, Shanghai 200240, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Jing X
authors.initials: JX
authors.lastname: Kang
authors.forename: Wenhai
authors.initials: W
authors.lastname: Xie
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Li
authors.forename: Changqing
authors.initials: C
authors.lastname: Wu
authors.forename: Bo
authors.initials: B
authors.lastname: Xu
authors.forename: Ji
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation; D005260:Female; D018703:Founder Effect; D015870:Gene Expression; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007621:Karyotyping; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism; D009866:Oogenesis / Q000235:genetics; D010053:Ovary / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D055647:Rats, Transgenic / Q000235:genetics*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D019098:Telomerase / Q000235:genetics / Q000378:metabolism
chemical_list: C573129:IFITM3 protein, rat; D008565:Membrane Proteins; D049452:Green Fluorescent Proteins; D019098:Telomerase
keywords: animal model; cell culture; cell proliferation; germ cells