title: Molecular arrangement between multivalent glycocluster and Pseudomonas aeruginosa LecA (PA-IL) by atomic force microscopy: influence of the glycocluster concentration.
abstract: New therapeutics strategy against cystic fibrosis seeks to prevent the adhesion of the bacterium Pseudomonas aeruginosa (PA) on the epithelial cells in the lungs. One of the factors that induces the adhesion is the interaction between natural glycocluster present on the cells and lectins such as the PA lectin LecA (PA-IL) present on the bacterium. By introducing synthetic glycoclusters with a great affinity with the lectin PA-IL, the adhesion can be prevented. In this study, we characterized, by atomic force microscopy, the interaction between a tetra-galactosylated glycocluster and the PA-IL lectin for high concentration of lectins (2.5 μM).We showed that the strong lectin/lectin interaction is reduced even for low concentration of glycoclusters (1 for 20 000 lectins). We assumed that it is due to the tensioactive behavior of the glycoclusters. It was shown that the arrangement of the created complexes induced different structures evolving from one-dimensional elongated aggregates to two-dimensional compact islands when increasing the glycocluster concentration. This evolution can be interpreted as the predominance of the glycocluster/lectin interaction.
authors.affiliation: Université de Lyon, Ecole Centrale de lyon, Institut des Nanotechnologies de Lyon (INL, UMR CNRS 5270), 36 avenue Guy de Collongue, 69134, Ecully, France; Laboratoire Interdisciplinaire Carnot de Bourgogne (ICB UMR CNRS 6303) - Dept Nanosciences/OSNC, Faculty of Sciences Mirande, Université de Bourgogne, 9 Avenue A. Savary, 21078, Dijon, France.
authors.forename: D
authors.initials: D
authors.lastname: Sicard
authors.forename: Y
authors.initials: Y
authors.lastname: Chevolot
authors.forename: E
authors.initials: E
authors.lastname: Souteyrand
authors.forename: A
authors.initials: A
authors.lastname: Imberty
authors.forename: S
authors.initials: S
authors.lastname: Vidal
authors.forename: M
authors.initials: M
authors.lastname: Phaner-Goutorbe
mesh_terms: D006023:Glycoproteins / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D018625:Microscopy, Atomic Force / Q000379:methods*; D011485:Protein Binding; D011550:Pseudomonas aeruginosa / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D037102:Lectins
keywords: AFM; Pseudomonas aeruginosa; cystic fibrosis; glycocluster; inter molecular interaction; lectin; protein