title: Plant regeneration from protoplasts of a wild lettuce species (Lactuca saligna L.).
abstract: Protoplasts isolated from young, unexpanded leaves of the wild lettuce species, Lactuca saligna, divided to give colonies, when plated at low density in KP8 medium solidified with 1% (w/v) agarose. Sustained colony development was dependent upon the incorporation of the bead culture approach. Plant regeneration, via organogenesis, followed the transfer of colonies firstly to K8 medium and then to M/S medium supplemented with IAA and 6-BAP, both solidified with 1% (w/v) agarose. Excised shoots were rooted on agar-solidified M/S medium lacking phytohormones. The potential of this species as a source of race-non-specific mildew resistance for transfer into lettuce (L. sativa) via somatic hybridisation is discussed.
authors.affiliation: Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, Nottingham, UK.
authors.forename: C
authors.initials: C
authors.lastname: Brown
authors.forename: J A
authors.initials: JA
authors.lastname: Lucas
authors.forename: J B
authors.initials: JB
authors.lastname: Power