title: Tonoplast fine structure and osmotic regulation in Porphyra umbilicalis.
abstract: In Porphyra, an intertidal red alga, the fine structure of the tonoplast was studied by freeze-fracture electron microscopy. It was shown that density and size of intramembraneous particles on the protoplasmic fracture face vary with external osmotic potential. The frequency of particles grouped in size classes (calculated per cell) increases with increasing osmotic stress and shows a maximum in 3 to 4 x artificial seawater medium ASP12. It is concluded that the intensity of tonoplast transport, which probably is enhanced with increasing osmotic stress from 1 to 4 x media, is most likely correlated with a change in membrane fine structure of the tonoplast.
authors.affiliation: Botanisches Institut der Tierärztlichen Hochschule Hannover, Bünteweg 17D, D-3000, Hannover 71, Federal Republic of Germany.
authors.forename: C
authors.initials: C
authors.lastname: Wiencke
authors.forename: A
authors.initials: A
authors.lastname: Läuchli