title: Expression system for production of bioactive compounds, recombinant human adiponectin, in the silk glands of transgenic silkworms.
abstract: Adiponectin is an adipocyte hormone involved in glucose and lipid metabolism. The aim of this study was to develop a human adiponectin expression system in transgenic silkworm using a human adiponectin expression vector. The silk gland of the silkworm is a highly specialized organ that has the wonderful ability to synthesize and secrete silk protein. To express human adiponectin in the silk gland of transgenic silkworm, targeting vectors pB-A3-adiponectin-IRES-RFP and pB-Ser1-adiponectin-IRES-RFP were constructed and then introduced into the silkworm pupa. The transgenic silkworms were verified by PCR and then generated. The level of adiponectin in the transgenic silkworm was 6-10 ng/50 mg of freeze-dried powder, and western blotting using an antibody against human adiponectin demonstrated a specific band with a molecular weight of 30 kDa in the silkworm. These results showed that human adiponectin introduced into the silkworm genome was expressed successfully on a large-scale.
authors.affiliation: College of Pharmacy, Sahmyook University, Hwarangro-815, Nowon-gu, Seoul, 139-742, Korea.
authors.forename: Seulmee
authors.initials: S
authors.lastname: Shin
authors.forename: Bong-Yoon
authors.initials: BY
authors.lastname: Kim
authors.forename: Hyung-Yook
authors.initials: HY
authors.lastname: Jeon
authors.forename: Aeri
authors.initials: A
authors.lastname: Lee
authors.forename: Sungwon
authors.initials: S
authors.lastname: Lee
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Sung
authors.forename: Chan-Su
authors.initials: CS
authors.lastname: Park
authors.forename: Chong-Kil
authors.initials: CK
authors.lastname: Lee
authors.forename: Hyunseok
authors.initials: H
authors.lastname: Kong
authors.forename: Youngcheon
authors.initials: Y
authors.lastname: Song
authors.forename: Kyungjae
authors.initials: K
authors.lastname: Kim
mesh_terms: D052242:Adiponectin / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified; D015153:Blotting, Western; D012831:Bombyx / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D006801:Humans; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; D011994:Recombinant Proteins