title: Stevia rebaudiana Bertoni as a natural antioxidant/antimicrobial for high pressure processed fruit extract: processing parameter optimization.
abstract: Response surface methodology was used to evaluate the optimal high pressure processing treatment (300-500 MPa, 5-15 min) combined with Stevia rebaudiana (Stevia) addition (0-2.5% (w/v)) to guarantee food safety while maintaining maximum retention of nutritional properties. A fruit extract matrix was selected and Listeria monocytogenes inactivation was followed from the food safety point of view while polyphenoloxidase (PPO) and peroxidase (POD) activities, total phenolic content (TPC) and antioxidant capacity (TEAC and ORAC) were studied from the food quality point of view. A combination of treatments achieved higher levels of inactivation of L. monocytogenes and of the oxidative enzymes, succeeding in completely inactivating POD and also increasing the levels of TPC, TEAC and ORAC. A treatment of 453 MPa for 5 min with a 2.5% (w/v) of Stevia succeeded in inactivating over 5 log cycles of L. monocytogenes and maximizing inactivation of PPO and POD, with the greatest retention of bioactive components.
authors.affiliation: Departamento de Conservación y Calidad, Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC), Carrer del Catedrátic Agustín Escardino Benlloch 7, 46980 Paterna, València, Spain.
authors.forename: Francisco José
authors.initials: FJ
authors.lastname: Barba
authors.forename: María Nieves
authors.initials: MN
authors.lastname: Criado
authors.forename: Clara Miracle
authors.initials: CM
authors.lastname: Belda-Galbis
authors.forename: María José
authors.initials: MJ
authors.lastname: Esteve
authors.forename: Dolores
authors.initials: D
authors.lastname: Rodrigo
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D029441:Carica / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology / Q000382:microbiology; D004156:Catechol Oxidase / Q000032:analysis; D032084:Citrus sinensis / Q000187:drug effects / Q000201:enzymology / Q000382:microbiology; D005519:Food Preservation / Q000379:methods*; D005520:Food Preservatives / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology / Q000382:microbiology; D008089:Listeria monocytogenes / Q000187:drug effects / Q000254:growth & development; D031022:Mangifera / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology / Q000382:microbiology; D009195:Peroxidase / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology*; D010940:Plant Proteins / Q000032:analysis; D036625:Stevia / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D005520:Food Preservatives; D010936:Plant Extracts; D010940:Plant Proteins; D004156:Catechol Oxidase; D009195:Peroxidase
keywords: High pressure processing; Listeria monocytogenes; Peroxidase; Phenolic compounds; Polyphenoloxidase; Response surface methodology; Stevia rebaudiana Bertoni; Total antioxidant capacity