title: Immunolocalization of cell cycle proteins (p57, p27, cyclin D3, PCNA and Ki67) in intrauterine growth retardation (IUGR) and normal human term placentas.
abstract: Placental development involves a series of events that depend on the coordinated action of proliferation, differentiation and invasion of trophoblasts. Studies on cell cycle related proteins controlling these events are fairly limited. It is still not fully determined how placental tissue proliferation is affected by intrauterine growth retardation (IUGR). Information on cell cycle related proteins that control these events is limited and how they are affected in IUGR is not fully understood. The aim of this study was to understand the role of cell cycle regulators in IUGR placentas and to determine the spatio-temporal immunolocalization of these cell cycle regulators in human IUGR and normal term placentas. Placental samples were stained immunohistochemically with PCNA, Ki67, cyclin D3, p27 and p57 antibodies and were examined by light microscopy. In all regions of IUGR placentas, PCNA, Ki67 and cyclin D3 staining intensities were statistically significantly decreased compared to normal controls. p27 staining intensity of the IUGR group was statistically significantly increased in villous parts and chorionic plates in comparison with the normal term placentas. Moreover, p57 staining intensity was statistically significantly increased in all parts of the IUGR group compared to controls. The observed placental abnormalities in IUGR placentas may be associated with arrest mechanisms affecting cell proliferation and cell cycle alterations in IUGR.
authors.affiliation: Department of Histology and Embryology, Medical Faculty, Akdeniz University, 07070 Antalya, Turkey.
authors.forename: Gozde
authors.initials: G
authors.lastname: Unek
authors.affiliation: Department of Histology and Embryology, Medical Faculty, Akdeniz University, 07070 Antalya, Turkey.
authors.forename: Asli
authors.initials: A
authors.lastname: Ozmen
authors.affiliation: Department of Obstetrics and Gynecology, Medical Faculty, Akdeniz University, 07070 Antalya, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Ozekinci
authors.affiliation: Department of Obstetrics and Gynecology, Medical Faculty, Akdeniz University, 07070 Antalya, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Sakinci
authors.affiliation: Department of Histology and Embryology, Medical Faculty, Akdeniz University, 07070 Antalya, Turkey. Electronic address: korgun@akdeniz.edu.tr.
authors.forename: Emin Turkay
authors.initials: ET
authors.lastname: Korgun
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D056743:Cyclin D3 / Q000378:metabolism*; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism*; D050761:Cyclin-Dependent Kinase Inhibitor p57 / Q000378:metabolism*; D005260:Female; D005317:Fetal Growth Retardation / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism / Q000473:pathology; D011247:Pregnancy; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D055815:Young Adult
chemical_list: D015415:Biomarkers; C534696:CCND3 protein, human; C493964:CDKN1C protein, human; D056743:Cyclin D3; D050761:Cyclin-Dependent Kinase Inhibitor p57; D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D050760:Cyclin-Dependent Kinase Inhibitor p27
keywords: Cyclin D3; IUGR; Ki67; PCNA; Placenta; p27; p57