title: A comparative immunocytochemical localization study of phosphoenolpyruvate carboxylase in leaves of higher plants.
abstract: The intracellular localization of phosphoenolpyruvate (PEP) carboxylase in plants belonging to the C4, Crassulacean acid metabolism (CAM) and C3 types was invetigated using an immunocytochemical method with an immune serum raised against the sorghum leaf enzyme. The plants studied were sorghum, maize (C4 type), kalanchoe (CAM type), french bean, and spinach (C3 type). In the green leaves of C4 plants, it was shown that the carboxylase was located in the mesophyll and stomatic cells, being largely cytosolic in the mesophyll cells. Similarly, in CAM plants, the enzyme was found mainly outside the chloroplasts. In contrast, in C3 plants, the PEP carboxylase appeared to be distributed between the cytosol and the chloroplasts of foliar parenchyma. Examination of sections from etiolated leaves showed fluorescence emission from etioplasts and cytosol for the parenchyma of french bean as well as for the bundle sheath and mesophyll of sorghum leaves. This data indicated that during the greening process photoregulation and evolution of PEP carboxylase is dependent on the tissue and on the metabolic type of the plant considered.
authors.affiliation: Laboratoire de Physiologie Végétale Métabolique, ERA CNRS no 799, Université de Paris Sud, Centre d'Orsay, F-91405, Orsay-Cedex, France.
authors.forename: C
authors.initials: C
authors.lastname: Perrot-Rechenmann
authors.forename: J
authors.initials: J
authors.lastname: Vidal
authors.forename: J
authors.initials: J
authors.lastname: Brulfert
authors.forename: A
authors.initials: A
authors.lastname: Burlet
authors.forename: P
authors.initials: P
authors.lastname: Gadal