title: Zinc finger protein 219-like (ZNF219L) and Sox9a regulate synuclein-γ2 (sncgb) expression in the developing notochord of zebrafish.
abstract: Zebrafish synuclein-γ2 (sncgb) has been reported to be expressed specifically in the notochord. However, the mechanism by which the sncgb gene promoter is regulated has not been described. In this paper, we demonstrate that Zinc finger protein 219-like (ZNF219L) and sox9a are involved in the regulation of sncgb gene expression. Furthermore, we observed that over-expression of both ZNF219L and Sox9a resulted in increased sncgb expression. In addition, ZNF219L is physically associated with Sox9a, and simultaneous morpholino knockdown of znf219L and sox9a caused a synergistic decrease of sncgb expression in the notochord. Taken together, our results reveal that coordination of ZNF219L with Sox9a is involved in the regulation of notochord-specific expression of sncgb.
authors.affiliation: Institute of Fisheries Sciences, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Huang-Wei
authors.initials: HW
authors.lastname: Lien
authors.forename: Chung-Hsiang
authors.initials: CH
authors.lastname: Yang
authors.forename: Chia-Hsiung
authors.initials: CH
authors.lastname: Cheng
authors.forename: Yung-Feng
authors.initials: YF
authors.lastname: Liao
authors.forename: Yu-San
authors.initials: YS
authors.lastname: Han
authors.forename: Chang-Jen
authors.initials: CJ
authors.lastname: Huang
mesh_terms: D000818:Animals; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D009672:Notochord / Q000196:embryology* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D055758:SOX9 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D051843:Synucleins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D055758:SOX9 Transcription Factor; C504918:Sox9a protein, zebrafish; D051843:Synucleins; D014157:Transcription Factors; D029961:Zebrafish Proteins; C588039:synuclein-gamma2 protein, zebrafish; C588040:znf219 protein, zebrafish
keywords: Notochord; Sox9a; Synuclein-γ2 (sncgb); ZNF219-like (ZNF219L); Zebrafish