title: Activity of imipenem against Klebsiella pneumoniae biofilms in vitro and in vivo.
abstract: Encapsulated Klebsiella pneumoniae has emerged as one of the most clinically relevant and more frequently encountered opportunistic pathogens in combat wounds as the result of nosocomial infection. In this report, we show that imipenem displayed potent activity against established K. pneumoniae biofilms under both static and flow conditions in vitro. Using a rabbit ear model, we also demonstrated that imipenem was highly effective against preformed K. pneumoniae biofilms in wounds.
authors.affiliation: Microbiology Branch, U.S. Army Dental and Trauma Research Detachment, Institute of Surgical Research, Fort Sam Houston, Texas, USA.
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Akhil K
authors.initials: AK
authors.lastname: Seth
authors.forename: Johnathan J
authors.initials: JJ
authors.lastname: Abercrombie
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Mustoe
authors.forename: Kai P
authors.initials: KP
authors.lastname: Leung
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D020405:Dermis / Q000187:drug effects* / Q000293:injuries / Q000382:microbiology; D004423:Ear / Q000293:injuries / Q000382:microbiology; D015378:Imipenem / Q000494:pharmacology*; D007710:Klebsiella Infections / Q000188:drug therapy* / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D062326:Re-Epithelialization / Q000187:drug effects / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D015378:Imipenem