title: Separation, enrichment and determination of ciprofloxacin using thermoseparating polymer aqueous two-phase system combined with high performance liquid chromatography in milk, egg, and shrimp samples.
abstract: Aqueous two-phase systems (ATPS) are potential industrial technology in separation and enrichment areas. However, the main problem is that ATPS could not be recycled, which results in a high cost and pollution of the environment. In this work, a new ATPS which formed by thermoseparating polymer EOPO with phosphate was developed for the separation and enrichment of ciprofloxacin (CIP). In the first procedure, the single factors have been discussed. The results showed that when the amounts of EOPO (80%, w/w) and K2HPO4 (55%, w/w) were 3.0 mL, 7.0 mL, respectively, pH=11, the extraction efficiency of CIP could reach 97.7%. In the second extraction, with the temperature induced, the extraction efficiency of CIP showed 85.6%. Additionally, the phase components were successfully recycled and reused for more than two times. The new method was successfully applied to the determination of CIP in real samples with detection limits of 6.8 ng g(-1).
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.forename: Juan
authors.initials: J
authors.lastname: Han
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Chengzhuo
authors.initials: C
authors.lastname: Sheng
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Guocai
authors.initials: G
authors.lastname: Zhang
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Yan
mesh_terms: D000818:Animals; D001156:Artemia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000032:analysis* / Q000302:isolation & purification*; D004531:Eggs / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D012758:Shellfish / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D011108:Polymers; D002939:Ciprofloxacin
keywords: ATPS; Ciprofloxacin; EOPO; HPLC; Secondary extraction