title: Resistant tobacco plants from protoplast-derived calluses selected for their resistance to Pseudomonas and Alternaria toxins.
abstract: Protoplast-derived calluses of tobacco (Nicotiana tabacum cv. 'Samsun') were selected for their resistance to toxins from Pseudomonas syringae pv. tabaci, which causes wildfire disease, and from Alternaria alternata pathotype tobacco, which causes brown spot. A number of plants were regenerated from each of the toxin-selected protoplast-derived calluses. A large percentage of the plants obtained from the second selection cycle calluses were resistant to infection by these pathogens. Resistance to wildfire disease, however, seems to be unrelated to resistance to brown spot disease. Variations in the morphological characteristics of the regenerated plants were found. Results of an assay of the R1 generation indicate that the resistance shown by R0 plants against both disease is heritable.
authors.affiliation: Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto University, Sakyo-ku, 606, Kyoto, Japan.
authors.forename: P
authors.initials: P
authors.lastname: Thanutong
authors.forename: I
authors.initials: I
authors.lastname: Furusawa
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto