title: Nitrotyrosine level was associated with mortality in patients with acute kidney injury.
abstract: BACKGROUND
  To examine the characteristics of oxidative stress in patients with acute kidney injury (AKI) and investigate the association between plasma nitrotyrosine levels and 90-day mortality in patients with AKI.
  
  
  RESULTS
  158 patients with hospital-acquired AKI were recruited to this prospective cohort study according to RIFLE (Risk, Injury, Failure, Lost or End Stage Kidney) criteria. Twelve critically ill patients without AKI and 15 age and gender-matched healthy subjects served as control. Plasma 3-nitrotyrosine was analyzed in relation to 90-day all cause mortality of patients with AKI. The patients with AKI were followed up for 90 days and grouped according to median plasma 3-nitrotyrosine concentrations. Highest 3-NT/Tyr was detected in patients with AKI compared with healthy subjects, and critically ill patients without AKI (ANOVA p<0.001). The 90-day survival curves of patients with high 3-NT/Tyr showed significant differences compared with the curves of individuals with low 3-NT/Tyr (p = 0.001 by log rank test). Multivariate analysis (Cox regression) revealed that 3-NT/Tyr (p = 0.025) was independently associated with mortality after adjustment for age, gender, sepsis and Acute Physiology and Chronic Health Evaluation (APACHE) II score.
  
  
  CONCLUSIONS
  There is excess plasma protein oxidation in patients with AKI, as evidenced by increased nitrotyrosine content. 3-NT/Tyr level was associated with mortality of AKI patients independent of the severity of illness.
authors.affiliation: Division of Nephrology, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qian
authors.forename: Huaizhou
authors.initials: H
authors.lastname: You
authors.forename: Qiuyu
authors.initials: Q
authors.lastname: Zhu
authors.forename: Shuai
authors.initials: S
authors.lastname: Ma
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Zheng
authors.forename: Junfeng
authors.initials: J
authors.lastname: Liu
authors.forename: Dingwei
authors.initials: D
authors.lastname: Kuang
authors.forename: Yong
authors.initials: Y
authors.lastname: Gu
authors.forename: Chuanming
authors.initials: C
authors.lastname: Hao
authors.forename: Feng
authors.initials: F
authors.lastname: Ding
mesh_terms: D058186:Acute Kidney Injury / Q000097:blood* / Q000401:mortality; D000704:Analysis of Variance; D016022:Case-Control Studies; D015331:Cohort Studies; D005260:Female; D006801:Humans; D008297:Male; D011446:Prospective Studies; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: C002744:3-nitrotyrosine; D014443:Tyrosine