title: Suspension and protoplast culture of hexaploid wheat (Triticum aestivum L.).
abstract: Suspension cultures have been initiated from embryogenic callus of hexaploid wheat (Triticum aestivum L.). Most commonly, these "suspensions" are composed of callus-like clusters (up to 2 mm in diameter). Two rapidly-growing lines (MBE6 and C82d) have been obtained, which consist of smaller aggregates of cytoplasmic cells, and these have been maintained for more than 4 years. These lines show very limited morphogenetic capacity and only a single plantlet has been regenerated, from line MBE6, after 9 months in culture. Protoplasts isolated from line MBE6 are unable to divide, but protoplasts from line C82d consistently undergo sustained divisions to form callus or secondary cell suspensions.
authors.affiliation: Biochemistry Department, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts, UK.
authors.forename: S E
authors.initials: SE
authors.lastname: Maddock