title: Do histopathologic findings improve by increasing the sample size in cholecystectomies?
abstract: BACKGROUND
  Gallbladder diseases present with cholelithiasis in a wide spectrum of adenomas and cancers. Two or three specimens are sampled in cholecystectomies in routine pathology practice. The aim of this study was to investigate the increase in frequency of histologic alterations in cholecystectomies, particularly precancerous lesions, by increasing the sample size to understand the carcinoma pathway.
  
  
  METHODS
  Cholecystectomies of 432 patients with pathology records and materials from two medical centers were collected, and two groups were created. Initial data with two or three samples were allocated to Group 1 and the new six samples with the initial ones were allocated to Group 2. Hematoxylin and eosin (HandE) sections were examined for histopathologic alterations, and periodic acid-Schiff (PAS) Alcian blue (pH 2.5) and high iron diamine (pH 2.5) stains were used to signify the mucin profile in case of metaplasias. For the comparison of findings, non-parametric tests, McNemar's tests, chi-squared tests and Fisher's exact test were performed.
  
  
  RESULTS
  Of the 432 patients, 308 (71.3%) patients were female and 124 (28.7%) patients were male. The mean age of patients was 47.9±14.6 years. Cholesterolosis was observed in 95 (22%) patients in Group 1 and 108 (25%) patients in Group 2. Gallstones were detected in 255 (59%) of the cholecystectomies. There was a significant difference between Group 1 and Group 2 by increasing the sample size when we compared cholesterolosis, metaplasia and polyps (P<0.05). Cholecystitis and dysplasia rates were the same in both of the groups. There was no cancer determined.
  
  
  CONCLUSIONS
  Increasing the sample size in cholecystectomies increased the diagnosis of some histologic alterations, but further studies with a larger number of samples over a longer period time might increase the ability to determine precancerous lesions and concomitants.
authors.forename: Tumay
authors.initials: T
authors.lastname: Ozgur
authors.forename: Serhat
authors.initials: S
authors.lastname: Toprak
authors.forename: Ali
authors.initials: A
authors.lastname: Koyuncuer
authors.forename: Muhammed
authors.initials: M
authors.lastname: Guldur
authors.forename: Gurman
authors.initials: G
authors.lastname: Bayraktar
authors.forename: Mehmet
authors.initials: M
authors.lastname: Yaldiz
mesh_terms: D000236:Adenoma / Q000473:pathology* / Q000601:surgery; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002763:Cholecystectomy; D005260:Female; D005500:Follow-Up Studies; D005706:Gallbladder Neoplasms / Q000145:classification / Q000473:pathology* / Q000601:surgery; D006801:Humans; D006965:Hyperplasia / Q000473:pathology* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D011230:Precancerous Conditions / Q000473:pathology* / Q000601:surgery; D011379:Prognosis; D011446:Prospective Studies; D018401:Sample Size; D055815:Young Adult