title: Encapsulation of laccase in silica colloidosomes for catalysis in organic media.
abstract: We report on the encapsulation of a laccase solution in silica colloidosomes of different shell structures and the study of catalytic performance of the encapsulated enzyme in toluene. The encapsulation is carried out by linking silica nanoparticles at the water/oil interface using hyperbranched polyethoxysiloxane (PEOS) in an aqueous-laccase-solution-in-toluene Pickering emulsion. By varying the weight ratio of silica particles to water (Rs/w), colloidosome shell can be adjusted from a particle monolayer up to a bilayer bound with a sandwiched nanoporous silica thin film formed from PEOS. The encapsulated laccase exhibits catalytic activity and reusability that are controlled by Rs/w. With the increase of Rs/w the reusability of the enzyme improves, meanwhile, its activity declines. This method allows fabricating enzyme microcapsules with tailored activity and controlled release properties.
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University , 2999 North Renmin Road, Shanghai 201620, China.
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.forename: Chunyan
authors.initials: C
authors.lastname: Hu
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Martin
authors.initials: M
authors.lastname: Möller
authors.forename: Kelu
authors.initials: K
authors.lastname: Yan
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Zhu
mesh_terms: D002384:Catalysis; D003102:Colloids / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D003102:Colloids; D014867:Water; D012822:Silicon Dioxide