title: Prevalence of Gardnerella vaginalis among women with lactobacillus-predominant vaginal flora.
abstract: OBJECTIVE
  To determine the prevalence of Gardnerella vaginalis in women with normal vaginal flora.
  
  
  METHODS
  Women without symptoms or signs of vaginal infection and five or fewer lifetime sexual partners were recruited for a longitudinal study of vaginal flora. Negative Amsel criteria and a Nugent score of 0-3 were required for enrolment. Vaginal specimens were self-collected daily for Gram stain and every 3 days for PCR for G vaginalis for 30 days. Women completed daily diaries recording sexual activity, symptoms and menses.
  
  
  RESULTS
  Twenty women were recruited for the study with 19 completing all specimens and 1 lost to follow-up. During the 30-day study period, 13/19 (68.4%) of women had normal Nugent scores (0-3) whereas 6/19 (31.6%) of women had at least 2 days of Nugent scores in the intermediate range (p=0.09). Among the 19 women, 9 (47%) were negative for G vaginalis by PCR throughout the study period whereas 10 (53%) had at least one specimen that demonstrated the presence of G vaginalis by PCR. Of those women with intermediate flora on Gram stain during the course of the study 5/6 (83.3%) were positive for G vaginalis while 5/13 (38.5%) of those women with only normal Nugent scores were positive for G vaginalis. Thus, 61.5% of women with normal Nugent scores had no evidence of G vaginalis by serial PCR.
  
  
  CONCLUSIONS
  Gardnerella may not be part of the normal flora in women with optimal vaginal health.
authors.affiliation: Department of Medicine/Infectious Diseases, University of Alabama at Birmingham, , Birmingham, Alabama, USA.
authors.forename: Jane R
authors.initials: JR
authors.lastname: Schwebke
authors.forename: Moira S
authors.initials: MS
authors.lastname: Flynn
authors.forename: Charles A
authors.initials: CA
authors.lastname: Rivers
mesh_terms: D005260:Female; D006194:Gardnerella vaginalis / Q000302:isolation & purification*; D016908:Gram-Positive Bacterial Infections / Q000453:epidemiology*; D006801:Humans; D007778:Lactobacillus / Q000302:isolation & purification*; D008137:Longitudinal Studies; D016133:Polymerase Chain Reaction; D015995:Prevalence; D014621:Vagina / Q000382:microbiology*; D016585:Vaginosis, Bacterial / Q000175:diagnosis* / Q000209:etiology
keywords: BACTERIAL VAGINOSIS; PCR; VAGINAL MICROBIOLOGY