title: A promising multiple-epitope recombinant vaccine against classical swine fever virus.
abstract: Classical swine fever (CSF) is a highly contagious and often fatal disease of swine. It is caused by classical swine fever virus (CSFV), one of the members of the genus Pestivirus of the Flaviviridae family. The development of a safe and effective vaccine against the CSF is critical to pandemic control, this article shows a tandem-repeat multiple-epitope recombinant vaccine can protect pigs from CSFV challenge. That was composed as following: two copies each of glycoprotein E2 residues 693-707, 241-276 and 770-781, and two copies amino acid residues 1446-1460 of the non-structural protein NS2-3. In the challenge test, all of the swine vaccinated with Chinese vaccine strain (C-strain) were fully protected from a challenge with CSFV. However, after three successive vaccinations with the multiple-epitope recombinant vaccine, three out of five pigs were protected from challenge with CSFV (in terms of both clinical signs and viremia). These results demonstrate that multiple-epitope recombinant vaccine which carrying the major CSFV epitopes can induce a high level of epitope-specific antibodies and exhibit a protective capability that parallels induced by C-strain to a certain extent.
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China. Electronic address: tianhong@caas.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Tian
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China.
authors.forename: Xiangming
authors.initials: X
authors.lastname: Hou
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China.
authors.forename: Jinyan
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China.
authors.forename: Youjun
authors.initials: Y
authors.lastname: Shang
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China.
authors.forename: Shuanghui
authors.initials: S
authors.lastname: Yin
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China.
authors.forename: Keshan
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China. Electronic address: hnxiangtao@163.com.
authors.forename: Xiangtao
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D006691:Classical Swine Fever / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D006692:Classical Swine Fever Virus / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D058873:Pandemics / Q000517:prevention & control / Q000662:veterinary*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction; D013552:Swine; D014611:Vaccination / Q000592:standards / Q000662:veterinary*; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*; D014766:Viremia / Q000662:veterinary / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D000939:Epitopes; D012367:RNA, Viral; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines
keywords: Antigen epitopes; B and T cell epitopes; Classical swine fever virus (CSFV); Multiple-epitope