title: Transformation of homozygous diploid potato with an Agrobacterium tumefaciens binary vector system by adventitious shoot regeneration on leaf and stem segments.
abstract: Transformed potato (Solanum tuberosum) plants were obtained from homozygous diploid potato by using a transformation procedure in combination with an adventitious shoot regeneration method. Leaf and stem explants were inoculated with an Agrobacterium tumefaciens strain which contained a binary vector (pVU 1011) carrying the neomycin phosphotransferase gene. Shoot regeneration most effectively on stem explants, occurred within six weeks directly from the explants without introducing a callus phase. A strong seasonal influence on transformation efficiencies was observed. Analysis of a number of randomly selected regenerated shoots for their ability to root and form shoots on kanamycin-containing medium shows that over 90% of the regenerated shoots obtained are transformed. In a number of shoots transformation was confirmed by a test for the presence and expression of the NPT-II gene.
authors.affiliation: Department of Genetics, University of Groningen, Kerklaan 30, 9751 NN, Haren, Netherlands.
authors.forename: R G
authors.initials: RG
authors.lastname: Visser
authors.forename: E
authors.initials: E
authors.lastname: Jacobsen
authors.forename: A
authors.initials: A
authors.lastname: Hesseling-Meinders
authors.forename: M J
authors.initials: MJ
authors.lastname: Schans
authors.forename: B
authors.initials: B
authors.lastname: Witholt
authors.forename: W J
authors.initials: WJ
authors.lastname: Feenstra