title: ZFP91-a newly described gene potentially involved in prostate pathology.
abstract: In search for novel molecular targets in benign prostate hyperplasia (BPH), a PCR Array based screening of 84 genes was performed. Of those, expression of ZFP91 (ZFP91 zinc finger protein) was notably upregulated. Limited data concerning the function of ZFP91 product show that it is a potential transcription factor upregulated in human acute myelogenous leukemia and most recently found to be the non-canonical NF-κB pathway regulator. In order to test this finding on a larger number of samples, prostate specimens were obtained from patients undergoing adenomectomy for BPH (n = 21), and as a control, from patients undergoing radical cystectomy for bladder cancer (prostates unchanged pathologically, n = 18). Similar studies were performed on cultured human prostate cancer cell lines: LNCaP, DU145, 22Rv1, PC-3; as well as normal prostate epithelial cells-PrEC. Methods employed included: Human Obesity PCR Array (Qiagen), QPCR and Western blotting. QPCR studies confirmed significant overexpression of ZFP91 in BPH samples. On a protein level, however, comparison between normal and BPH prostates revealed insignificant differences. As for prostate cell lines examined, all expressed ZFP91 mRNA. Western blotting analysis showed markedly higher protein levels of ZFP91 in all cancer cell lines in comparison with normal (PrEC) cells. In conclusion, the upregulated ZFP91 mRNA in BPH, not accompanied by parallel changes in ZFP91 protein levels, together with ZFP91 protein abundance in prostate cancer cell lines suggest ZFP91 involvement in these prostate diseases.
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Swiecicki St., 60-781, Poznan, Poland, paschkelukasz@gmail.com.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Paschke
authors.forename: Marcin
authors.initials: M
authors.lastname: Rucinski
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Ziolkowska
authors.forename: Tomasz
authors.initials: T
authors.lastname: Zemleduch
authors.forename: Witold
authors.initials: W
authors.lastname: Malendowicz
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Kwias
authors.forename: Ludwik K
authors.initials: LK
authors.lastname: Malendowicz
mesh_terms: D004847:Epithelial Cells / Q000473:pathology; D006801:Humans; D008297:Male; D011467:Prostate / Q000473:pathology*; D011470:Prostatic Hyperplasia / Q000235:genetics / Q000473:pathology; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D014407:Tumor Cells, Cultured; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D044767:Ubiquitin-Protein Ligases; C553044:ZFP91 protein, human