title: Effects of recombinant human interleukin-10 on Treg cells, IL-10 and TGF-β in transplantation of rabbit skin.
abstract: The current study aimed to investigate the rejection and survival time of grafted skin, and the changes of Treg cells, interleukin 10 (IL-10) and transforming growth factor-β (TGF-β) in peripheral blood following skin transplantation with recombinant human interleukin-10 (rhIL-10) or cyclosporin A (CsA), as well as the role of IL-10 in immunological rejection mechanisms. A total of 36 rabbits were divided into two groups. The skin of a donor rabbit was transplanted onto the back of one receptor rabbit. Receptors were randomly divided into six groups, including rhIL-10 low-dose (5 µg/kg/d), rhIL-10 high-dose (10 µg/kg/d), CsA low-dose (5 mg/kg/d), CsA high-dose (10 mg/kg/d), rhIL-10 (5 µg/kg/d) and CsA (5 mg/kg/d) and negative control normal saline (NS; 1 ml/d). All groups received intramuscular drug injection for ten days, beginning one day prior to skin transplantation surgery. Following transplantation, each rabbit's peripheral blood was collected at different times. The changes of CD4+CD25+ regulatory T cells, IL-10 and TGF-β were determined by flow cytometry and enzyme-linked immunosorbent assay. When compared with the control group, the rejection and survival times of the experimental groups were longer following skin graft. Compared with the two CsA groups and the control group, the proportion of CD4+CD25+ regulatory T cells of rhIL-10 groups was significantly upregulated on the 4th and 7th days following surgery. However, TGF-β levels were not significantly different. Data suggested that the concentration of IL-10 was positively correlated with the proportion of CD4+CD25+ regulatory T cells. In addition, IL-10 may delay the rejection time of rabbit skin transplantation and prolong the survival time. Thus, the role of IL-10 in inhibited allograft rejection may be associated with CD4+CD25+ regulatory T cells and IL-10, and may be independent of TGF-β.
authors.affiliation: The Border Armed Police Central Hospital of Guangdong Province, Shenzhen, Guangdong 518023, P.R. China.
authors.forename: Kai Shan
authors.initials: KS
authors.lastname: Liu
authors.affiliation: College of Life Science and Teaching, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
authors.forename: Xiao Qin
authors.initials: XQ
authors.lastname: Fan
authors.affiliation: Department of Basic Teaching, Zunyi Medical College, Zhuhai Campus, Zhuhai, Guizhou 519041, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Diseases, Central People's Hospital of Zhanjiang, Zhanjiang, Guangdong 524037, P.R. China.
authors.forename: Qiong Na
authors.initials: QN
authors.lastname: Wen
authors.affiliation: Department of Surgical Laboratory, Zunyi Medical College Zhuhai Campus, Zhuhai, Guizhou 519041, P.R. China.
authors.forename: Ji Hong
authors.initials: JH
authors.lastname: Feng
authors.affiliation: Department of Immunology, Institute of Basic Medical Science, Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China.
authors.forename: Fu Chao
authors.initials: FC
authors.lastname: Chen
authors.affiliation: Department of Immunology, Institute of Basic Medical Science, Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China.
authors.forename: Jun Min
authors.initials: JM
authors.lastname: Luo
authors.affiliation: Department of Immunology, Institute of Basic Medical Science, Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China.
authors.forename: Wan Bang
authors.initials: WB
authors.lastname: Sun
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D016572:Cyclosporine / Q000494:pharmacology; D006084:Graft Rejection / Q000188:drug therapy / Q000276:immunology; D006801:Humans; D016753:Interleukin-10 / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism*; D053645:Interleukin-2 Receptor alpha Subunit / Q000187:drug effects / Q000276:immunology; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism*; D016038:Skin Transplantation / Q000379:methods*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D053645:Interleukin-2 Receptor alpha Subunit; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D016572:Cyclosporine