title: Overexpression of UDP-glucose pyrophosphorylase gene could increase cellulose content in Jute (Corchorus capsularis L.).
abstract: In this study, the full-length cDNA of the UDP-glucose pyrophosphorylase gene was isolated from jute by homologous cloning (primers were designed according to the sequence of UGPase gene of other plants) and modified RACE techniques; the cloned gene was designated CcUGPase. Using bioinformatic analysis, the gene was identified as a member of the UGPase gene family. Real-time PCR analysis revealed differential spatial and temporal expression of the CcUGPase gene, with the highest expression levels at 40 and 120d. PCR and Southern hybridization results indicate that the gene was integrated into the jute genome. Overexpression of CcUGPase gene in jute revealed increased height and cellulose content compared with control lines, although the lignin content remained unchanged. The results indicate that the jute UGPase gene participates in cellulose biosynthesis. These data provide an important basis for the application of the CcUGPase gene in the improvement of jute fiber quality.
authors.affiliation: Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
authors.forename: Gaoyang
authors.initials: G
authors.lastname: Zhang
authors.forename: Jianmin
authors.initials: J
authors.lastname: Qi
authors.forename: Jiantang
authors.initials: J
authors.lastname: Xu
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Niu
authors.forename: Yujia
authors.initials: Y
authors.lastname: Zhang
authors.forename: Aifen
authors.initials: A
authors.lastname: Tao
authors.forename: Liwu
authors.initials: L
authors.lastname: Zhang
authors.forename: Pingping
authors.initials: P
authors.lastname: Fang
authors.forename: Lihui
authors.initials: L
authors.lastname: Lin
mesh_terms: D002482:Cellulose / Q000032:analysis / Q000096:biosynthesis*; D003001:Cloning, Molecular; D032381:Corchorus / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D008031:Lignin / Q000032:analysis / Q000096:biosynthesis; D010802:Phylogeny; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000201:enzymology / Q000235:genetics; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase / Q000096:biosynthesis* / Q000145:classification / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D002482:Cellulose; D008031:Lignin; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase
keywords: ADP-glucose pyrophosphorylase; AGPase; CcUGPase; Cellulose; Jute; PPi; Quality; Southern hybridization; UDP; UDP-glucose pyrophosphorylase; UDPG; UGPase; UTP; d; days; pyrophosphate; uridine diphosphate; uridine diphosphate glucose; uridine triphosphate