title: Synthesis and properties of novel biomimetic and thermo-responsive dextran-based biohybrids.
abstract: A new class of biodegradable, biomimetic and thermo-responsive dextran/synthetic glycopolymer biohybrids (dextran-graft-poly(lactobionamidoethyl methacrylate)-block-poly(di(ethylene glycol) methyl ether methacrylate), dextran-g-(PLAMA-b-PDEGMA)), was synthesized by the direct atom transfer radical polymerization (ATRP) of unprotected lactobionamidoethyl methacrylate (LAMA) glycomonomer and di(ethylene glycol) methyl ether methacrylate (DEGMA) monomer. The dextran macroinitiator for ATRP was prepared by partial esterification of the hydroxyl groups of the polysaccharide with 2-bromo-2-methylpropionic acid (BrMPA). The biohybrids containing PDEGMA segments exhibited a lower critical solution temperature (LCST) behavior, which changed from unimers to aggregates in solutions. Moreover, it was demonstrated that these biohybrids had specific biomolecular recognition with ricinus communis agglutinin 120 (RCA₁₂₀) in comparison with bovine serum albumin (BSA). Furthermore, these biohybrids showed good biocompatibility in the cytotoxicity assays. This hopefully provides a platform for targeted drug delivery and studying the biomolecular recognition between sugar and lectin.
authors.affiliation: Institute of Materials Science and Engineering, Jilin University, Changchun 130023, PR China; State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Li
authors.forename: Danfeng
authors.initials: D
authors.lastname: Pei
authors.forename: Qingrong
authors.initials: Q
authors.lastname: Huang
authors.forename: Tongfei
authors.initials: T
authors.lastname: Shi
authors.forename: Guo
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000818:Animals; D032701:Biomimetics; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D003911:Dextrans / Q000737:chemistry*; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D058610:Isobutyrates / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008689:Methacrylates / Q000737:chemistry*; D037121:Plant Lectins / Q000737:chemistry*; D058105:Polymerization; D012710:Serum Albumin, Bovine / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D003911:Dextrans; D004337:Drug Carriers; D058610:Isobutyrates; D008689:Methacrylates; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D012710:Serum Albumin, Bovine
keywords: Atom transfer radical polymerization; Biodegradable; Dextran; Glycopolymer; Nanoparticles; Polysaccharide