title: SASD: the Synthetic Alternative Splicing Database for identifying novel isoform from proteomics.
abstract: BACKGROUND
  Alternative splicing is an important and widespread mechanism for generating protein diversity and regulating protein expression. High-throughput identification and analysis of alternative splicing in the protein level has more advantages than in the mRNA level. The combination of alternative splicing database and tandem mass spectrometry provides a powerful technique for identification, analysis and characterization of potential novel alternative splicing protein isoforms from proteomics.
  
  
  RESULTS
  We used a three-step pipeline to create a synthetic alternative splicing database (SASD) to identify novel alternative splicing isoforms and interpret them at the context of pathway, disease, drug and organ specificity or custom gene set with maximum coverage and exclusive focus on alternative splicing. First, we extracted information on gene structures of all genes in the Ensembl Genes 71 database and incorporated the Integrated Pathway Analysis Database. Then, we compiled artificial splicing transcripts. Lastly, we translated the artificial transcripts into alternative splicing peptides.
  
  
  CONCLUSIONS
  The SASD provides the scientific community with an efficient means to identify, analyze, and characterize novel Exon Skipping and Intron Retention protein isoforms from mass spectrometry and interpret them at the context of pathway, disease, drug and organ specificity or custom gene set with maximum coverage and exclusive focus on alternative splicing.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.forename: Renee
authors.initials: R
authors.lastname: Drabier
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D019295:Computational Biology / Q000379:methods; D030561:Databases, Nucleic Acid; D005091:Exons; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D010455:Peptides / Q000737:chemistry / Q000235:genetics; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D040901:Proteomics / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D053719:Tandem Mass Spectrometry
chemical_list: D010455:Peptides; D020033:Protein Isoforms; D011506:Proteins; D012333:RNA, Messenger