title: Ommexecha virens (Thunberg, 1824) and Descampsacris serrulatum (Serville, 1831) (Orthoptera, Ommexechidae): karyotypes, constitutive heterochromatin and nucleolar organizing regions.
abstract: Chromosomes of Ommexecha virens and Descampsacris serrulatum (Ommexechidae) were analyzed through conventional staining, C-banding, base specific fluorochromes, silver nitrate impregnation (AgNO3), and fluorescent in situ hybridization (FISH) with probe for 45S rDNA. The two species presented diploid number 2n= 23,X0 in males and acrocentric autosomes, except the pair one that presented submetacentric morphology. The X chromosome has distinct morphology in the two analyzed species, being a medium acrocentric in Ommexecha virens and large submetacentric in Descampsacris serrulatum. The C-banding revealed pericentromeric blocks of constitutive heterochromatin (CH) in all the chromosomes of Descampsacris serrulatum. For Ommexecha virens it was evidenced that the blocks of CH are preferentially located in the pericentromeric area (however some bivalents presents additional blocks) or in different positions. The staining with CMA3/DA/DAPI showed GC rich CH blocks (CMA3+) in some chromosomes of the two species. The nucleolar organizer regions (NORs) were located in the bivalents L2, S9, S10 of Ommexecha virens and M5, M6, M7, S11 of Descampsacris serrulatum. The FISH for rDNA showed coincident results with the pattern of active NORs revealed by AgNO3. This work presents the first chromosomal data, obtained through differential cytogenetics techniques in Ommexechidae, contributing to a better characterization of karyotypic evolution for this grasshopper family.
authors.affiliation: Departamento de Genética, Centro de Ciências Biológicas/CCB, Universidade Federal de Pernambuco/ UFPE, Av. Prof. Moraes Rego s/n, Recife, Pernambuco, Brasil CEP:50732-970.
authors.forename: D B
authors.initials: DB
authors.lastname: Carvalho
authors.forename: M F
authors.initials: MF
authors.lastname: Rocha
authors.forename: V
authors.initials: V
authors.lastname: Loreto
authors.forename: A E B
authors.initials: AE
authors.lastname: Silva
authors.forename: M J
authors.initials: MJ
authors.lastname: Souza
keywords: Descampsacris serrulatum; FISH; Ommexecha virens; Ommexechidae; grasshopper; rDNA