title: Carnitine long-chain acyltransferase and oxidation of palmitate, palmitoyl coenzyme A and palmitoylcarnitine by pea mitochondria preparations.
abstract: Palmitoylcarnitine was oxidised by pea mitochondria.L-carnitine was an essential addition for the oxidation of palmitate or palmitoylCoA. When palmitate was sole substrate, ATP and Mg(2+) were also essential additives for maximum oxidation. Additions of CoA inhibited the oxidation of palmitate. It was shown that CoA was acting as a competitive inhibitor of the carnitine-stimulated O2 uptake. It is suggested that palmitoylacarnitine and carnitine passed through the mitochondrial barrier with ease but palmitoylCoA and CoA did not. The presence of carnitine long-chain acyl (palmitoyl)transferase (EC 2.3.1.21) in pea-cotyledon mitochondria was shown. This enzyme may play a role in the transport of long-chain acyl groups through membrane barriers.
authors.affiliation: Department of Plant Biology, Newcastle University, NE1 7RU, Newcastle upon Tyne, UK.
authors.forename: C
authors.initials: C
authors.lastname: Wood
authors.forename: M N
authors.initials: MN
authors.lastname: Jalil
authors.forename: I
authors.initials: I
authors.lastname: McLaren
authors.forename: B C
authors.initials: BC
authors.lastname: Yong
authors.forename: A
authors.initials: A
authors.lastname: Ariffin
authors.forename: P H
authors.initials: PH
authors.lastname: McNeil
authors.forename: N
authors.initials: N
authors.lastname: Burgess
authors.forename: D R
authors.initials: DR
authors.lastname: Thomas