title: Bone marrow mesenchymal stromal cells isolated from multiple sclerosis patients have distinct gene expression profile and decreased suppressive function compared with healthy counterparts.
abstract: Multiple sclerosis (MS) is a chronic inflammatory autoimmune disease of the central nervous system, due to an immune reaction against myelin proteins. Multipotent mesenchymal stromal cells (MSCs) present immunosuppressive effects and have been used for the treatment of autoimmune diseases. In our study, gene expression profile and in vitro immunomodulatory function tests were used to compare bone marrow-derived MSCs obtained from MS patients, at pre- and postautologous hematopoietic stem cell transplantation (AHSCT) with those from healthy donors. Patient MSCs comparatively exhibited i) senescence in culture; ii) similar osteogenic and adipogenic differentiation potential; iii) decreased expression of CD105, CD73, CD44, and HLA-A/B/C molecules; iv) distinct transcription at pre-AHSCT compared with control MSCs, yielding 618 differentially expressed genes, including the downregulation of TGFB1 and HGF genes and modulation of the FGF and HGF signaling pathways; v) reduced antiproliferative effects when pre-AHSCT MSCs were cocultured with allogeneic T-lymphocytes; vi) decreased secretion of IL-10 and TGF-β in supernatants of both cocultures (pre- and post-AHSCT MSCs); and vii) similar percentages of regulatory cells recovered after MSC cocultures. The transcriptional profile of patient MSCs isolated 6 months posttransplantation was closer to pre-AHSCT samples than from healthy MSCs. Considering that patient MSCs exhibited phenotypic changes, distinct transcriptional profile and functional defects implicated in MSC immunomodulatory and immunosuppressive activity, we suggest that further MS clinical studies should be conducted using allogeneic bone marrow MSCs derived from healthy donors. We also demonstrated that treatment of MS patients with AHSCT does not reverse the transcriptional and functional alterations observed in patient MSCs.
authors.affiliation: Center for Cell-Based Research, Regional Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil.
authors.forename: Gislane L V
authors.initials: GL
authors.lastname: de Oliveira
authors.forename: Kalil W A
authors.initials: KW
authors.lastname: de Lima
authors.forename: Amanda M
authors.initials: AM
authors.lastname: Colombini
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Pinheiro
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Panepucci
authors.forename: Patrícia V B
authors.initials: PV
authors.lastname: Palma
authors.forename: Doralina G
authors.initials: DG
authors.lastname: Brum
authors.forename: Dimas T
authors.initials: DT
authors.lastname: Covas
authors.forename: Belinda P
authors.initials: BP
authors.lastname: Simões
authors.forename: Maria C
authors.initials: MC
authors.lastname: de Oliveira
authors.forename: Eduardo A
authors.initials: EA
authors.lastname: Donadi
authors.forename: Kelen C R
authors.initials: KC
authors.lastname: Malmegrim
mesh_terms: D000328:Adult; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D016000:Cluster Analysis; D018920:Coculture Techniques; D016207:Cytokines / Q000032:analysis; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000378:metabolism / Q000473:pathology* / Q000628:therapy; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D059467:Transcriptome; D055815:Young Adult
chemical_list: D016207:Cytokines