title: Alkaloids enriched extract from Dendrobium nobile Lindl. attenuates tau protein hyperphosphorylation and apoptosis induced by lipopolysaccharide in rat brain.
abstract: Neurofibrillary tangles, one of the characteristic pathological features of Alzheimer's disease (AD), are composed of paired helical filaments mainly with hyperphosphorylated tau protein. Inhibition of the hyperphosphorylation of tau protein is an effective therapy for AD. The current study was designed to investigate the protective effects of alkaloids enriched extract from Dendrobium Nobile Lindl. (EDNLA), a Chinese medicinal herb, on hyperphosphorylation of tau protein in AD brain. Rats were administrated intragastrically with different doses of DNLA (20, 40 mg/kg) every 8h for one day, followed by lipopolysaccharide (LPS, 100 μg) injecting into the bilateral ventricle. Two hours later, the hippocampi of each group were collected to examine the hyperphosphorylated tau protein by western blotting. Additional rats were treated by EDNLA thrice daily for one week, to examine the effects on LPS-induced apoptosis in the brain. LPS injection significantly increased the expression of hyperphosphorylated tau protein at Ser396, Ser199-202, Ser404, Thr231, Thr205 sites and GSK-3β increased, LPS also induced apoptosis in the brain. EDNLA dramatically ameliorated these abnormal changes (P<0.05). The present study demonstrated that EDNLA attenuates LPS-induced hyperphosphorylation of tau protein in rat's hippocampus and protects against LPS-induced apoptosis in rat brain.
authors.affiliation: Department of Pharmacology, Zunyi Medical College, Zunyi, Guizhou 563000, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Pharmacology, Zunyi Medical College, Zunyi, Guizhou 563000, China.
authors.forename: Qihai
authors.initials: Q
authors.lastname: Gong
authors.affiliation: Department of Pharmacology, Zunyi Medical College, Zunyi, Guizhou 563000, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Pharmacology, Zunyi Medical College, Zunyi, Guizhou 563000, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Pharmacology, Zunyi Medical College, Zunyi, Guizhou 563000, China.
authors.forename: Yuanfu
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Pharmacology, Zunyi Medical College, Zunyi, Guizhou 563000, China. Electronic address: shijs@zmc.edu.cn.
authors.forename: Jingshan
authors.initials: J
authors.lastname: Shi
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy* / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D031667:Dendrobium; D004353:Drug Evaluation, Preclinical; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D008070:Lipopolysaccharides; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D016875:tau Proteins / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D010936:Plant Extracts; D016875:tau Proteins
keywords: Dendrobium nobile Lindl. alkaloids; Hyperphosphorylation; Lipopolysaccharide; Rat; Tau protein