title: Lectin levels in tissues of cultured immature wheat embryos.
abstract: Levels of wheat germ agglutinin have been determined by radioimmunoassay in tissues of immature wheat embryos cultured under different conditions in order to determine the suitability of the lectin as a marker for somatic embryogenesis. Embryos cultured on media favouring continued embryo development accumulated lectin in a similar manner to zygotic embryos in planta unless precocious germination occurred. Embryos cultured on media containing 2,4-D produced callus, and some of this developed somatic embryos. Both embryogenic and non-embryogenic callus contained WGA, that in non-embryogenic callus possibly arising from developmentally arrested root primordia.
authors.affiliation: Department of Biochemistry, University of Leeds, LS2 9JT, Leeds, UK.
authors.forename: P C
authors.initials: PC
authors.lastname: Morris
authors.forename: S E
authors.initials: SE
authors.lastname: Maddock
authors.forename: M G
authors.initials: MG
authors.lastname: Jones
authors.forename: D J
authors.initials: DJ
authors.lastname: Bowles