title: Sequence diversity of polysomal mRNAs in roots and shoots of etiolated and greened pea seedlings.
abstract: The sequence complexity and abundance of polysomal mRNA populations of pea seedlings were measured using RNA excess hybridization to both single-copy DNA and complementary DNA. The estimated sequence complexity of the polysomal mRNA populations was 2.5·10(7) nucleotides or 19,400 different mRNAs of average size. Since the haploid genome size of pea was found to be 4.0·10(9) nucleotide pairs, only 0.62% of the total haploid genome of pea was transcribed into polysomal mRNA. The roots and shoots of 4-d etiolated and light-grown seedlings contained similar numbers of diverse mRNAs. The RNA excess hybridizations, using single-copy DNA enriched for sequences transcribed in either light-grown shoots or etiolated roots and single-copy DNA depleted of such sequences, indicated that at least 92% of the sequence complexity of polysomal mRNAs was identical in roots and shoots irrespective of the presence of a functional photosynthetic system. In contrast, RNA excess hybridization to complementary DNA revealed that 21% of the polysomal polyadenylated mRNA mass found in light-grown shoots was absent in etiolated roots. The kinetics of these hybridizations indicated that this was due to the appearance of a limited number of abundant mRNAs under conditions of illumination.
authors.affiliation: Biologisch Centrum, Rijksuniversiteit Groningen, Kerklaan 30, NL-9751 NN, Haren, The Netherlands.
authors.forename: S C
authors.initials: SC
authors.lastname: de Vries
authors.forename: J
authors.initials: J
authors.lastname: Springer
authors.forename: J G
authors.initials: JG
authors.lastname: Wessels