title: Bioconjugation of gold and silver nanoparticles synthesized by Fusarium oxysporum and their use in rapid identification of Candida species by using bioconjugate-nano-polymerase chain reaction.
abstract: We developed a Bioconjugate-Nano-PCR as a rapid and specific method for identification of Candida species in less time. This requires very low concentration of master mix and DNA sample of Candida albicans in conjugation with gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs). We report a modification of the PCR assay with nanoparticles that allows the detection of high fidelity amplification of ITS-rDNA and beta (beta) tubulin gene of Candida species from low concentrated DNA in short period. We synthesized and characterized the covalently attached 34 nm (AuNPs) and 35 nm of (AgNPs) and conjugated with C. albicans DNA sample, which is used as a template for PCR. The use of this nanoparticle modified template improves the sensitivity and specificity of the traditional PCR assay with very low cycles which is very helpful in molecular diagnostics and therapeutics. It proves to be an effective method for identification of Candida species with low concentration of DNA. This type of PCR assay is useful for detection of target gene by enhancing the specificity of the target gene and is less time consuming.
authors.affiliation: Department of Biotechnology, SGB Amravati University, Maharashtra, Amravati 444602, India.
authors.forename: Sunita
authors.initials: S
authors.lastname: Bansod
authors.forename: Shital
authors.initials: S
authors.lastname: Bonde
authors.forename: Vaibhav
authors.initials: V
authors.lastname: Tiwari
authors.forename: Manisha
authors.initials: M
authors.lastname: Bawaskar
authors.forename: Shivaji
authors.initials: S
authors.lastname: Deshmukh
authors.forename: Swapnil
authors.initials: S
authors.lastname: Gaikwad
authors.forename: Aniket
authors.initials: A
authors.lastname: Gade
authors.forename: Mahendra
authors.initials: M
authors.lastname: Rai
mesh_terms: D002175:Candida / Q000302:isolation & purification*; D002177:Candidiasis / Q000175:diagnosis; D004271:DNA, Fungal / Q000032:analysis; D005670:Fusarium / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D008828:Microbiological Techniques / Q000295:instrumentation / Q000379:methods*; D058726:Nanoconjugates / Q000737:chemistry*; D010316:Particle Size; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*; D013997:Time Factors
chemical_list: D004271:DNA, Fungal; D058726:Nanoconjugates; D012834:Silver; D006046:Gold