title: A new method for the purification of the different stages of carrot embryoids.
abstract: An easy method is presented for the purification of the different stages of carrot embryoids. This is based on a synchronization of the regenerating culture and on a filtration through filters of various pore sizes. A differential sedimentation was used for removing undifferentiated cells. At the end of the process, the different stages: globular, heart- and torpedo-shaped were obtained with a degree of purity that always exceeded 90%. This method can be used for the separation of relatively large numbers of embryoids (from thousands to a million) of haploid and diploid carrot lines and is very gentle on embryoids in that it does not affect their viability or further development.
authors.affiliation: Istituto di Mutagenesi e Differenziamento, C.N.R., Via Svezia, 10, I-56100, Pisa, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Giuliano
authors.forename: D
authors.initials: D
authors.lastname: Rosellini
authors.forename: M
authors.initials: M
authors.lastname: Terzi