title: Distribution and diversity of nitrite-dependent anaerobic methane-oxidising bacteria in the sediments of the Qiantang River.
abstract: Nitrite-dependent anaerobic methane oxidation (n-damo) process was reported to be mediated by "Candidatus Methylomirabilis oxyfera", which belongs to the candidate phylum NC10. M. oxyfera-like bacteria have been detected in lake ecosystems, while their distribution, diversity and abundance in river ecosystems have not been well studied. In this study, both the 16S rRNA and the pmoA molecular biomarkers confirmed the presence of diverse NC10 phylum bacteria related to M. oxyfera in a river ecosystem-the Qiantang River, Zhejiang Province (China). Phylogenetic analysis of 16S rRNA genes demonstrated that the recovered M. oxyfera-like sequences could be grouped into several distinct clusters that exhibited 89.8% to 98.9% identity to the M. oxyfera 16S rRNA gene. Similarly, several different clusters of pmoA gene sequences were observed, and these clusters displayed 85.1-95.4% sequence identity to the pmoA gene of M. oxyfera. Quantitative PCR showed that the abundance of M. oxyfera-like bacteria varied from 1.32 ± 0.16 × 10(6) to 1.03 ± 0.12 × 10(7) copies g (dry weight)(-1). Correlation analysis demonstrated that the total inorganic nitrogen content, the ammonium content and the organic content of the sediment were important factors affecting the distribution of M. oxyfera-like bacterial groups in the examined sediments. This study demonstrated the distribution of diverse M. oxyfera-like bacteria and their correlation with environmental factors in Qiantang River sediments.
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Li-dong
authors.initials: LD
authors.lastname: Shen
authors.forename: Shuai
authors.initials: S
authors.lastname: Liu
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhu
authors.forename: Xiao-yu
authors.initials: XY
authors.lastname: Li
authors.forename: Chen
authors.initials: C
authors.lastname: Cai
authors.forename: Dong-qing
authors.initials: DQ
authors.lastname: Cheng
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Lou
authors.forename: Xiang-yang
authors.initials: XY
authors.lastname: Xu
authors.forename: Ping
authors.initials: P
authors.lastname: Zheng
authors.forename: Bao-lan
authors.initials: BL
authors.lastname: Hu
mesh_terms: D000693:Anaerobiosis; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D017753:Ecosystem; D019015:Geologic Sediments / Q000382:microbiology*; D008697:Methane / Q000737:chemistry; D009573:Nitrites / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D009573:Nitrites; D012336:RNA, Ribosomal, 16S; D008697:Methane