title: Somatic embryogenesis and plant regeneration in an interspecific hybrid of Oryza.
abstract: An embryogenic callus was obtained from immature panicle of an interspecific hybrid (Oryza sativa x O. latifolia) F1. The medium consisted of HE salts supplemented with 2,4-D, NAA (each 2 mg/l), kinetin (3 mg/l), yeast extract (1360 mg/l) and casein hydrolyzate (300 mg/l). The callus was milk-white in colour compact and granulate in texture. Various developmental stage of embryoid, such as globular, heart-shape, scutellum-shape and mature embryoid were observed in an embryogenic callus. Plantlets were successfully regenerated from 1-month-old callus with more than 80% regenerational frequency in each subculture for 12 passages.
authors.affiliation: South China Institute of Botany, Academia Sinica, Guang-zhou, People's Republic of China.
authors.forename: D H
authors.initials: DH
authors.lastname: Ling
authors.forename: W Y
authors.initials: WY
authors.lastname: Chen
authors.forename: M F
authors.initials: MF
authors.lastname: Chen
authors.forename: Z R
authors.initials: ZR
authors.lastname: Ma