title: cDNA cloning of an mRNA encoding a sulfur-rich 10 kDa prolamin polypeptide in rice seeds.
abstract: Using a rice maturing seed pUC9 expression library, we isolated a cDNA clone corresponding to 10 kDa sulfurrich prolamin by immunoscreening. A longer cDNA clone was obtained from a λgtll library by plaque hybridization using this (32)P-labeled cDNA as a probe. A polypeptide sequence composed of 134 amino acids was deduced from the nucleotide sequence. A 24 amino acid signal peptide was assigned by computer calculation for the membrane spanning region and Edman sequencing of the purified mature polypeptide. Remarkably, 20% of methionine and 10% of cysteine were found in the mature polypeptide as well as high contents of glutamine, and hydrophobic amino acids. Part of the amino acid sequence was homologous with a conserved cysteine-rich region found in other plant prolamins. Two repeats of amino acid sequence were found in the polypeptide.
authors.affiliation: Department of Biochemistry, College of Agricultural Chemistry, Kyoto Prefectural University ShimogamoShimogamoShimogamo, 606, Kyoto, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Masumura
authors.forename: D
authors.initials: D
authors.lastname: Shibata
authors.forename: T
authors.initials: T
authors.lastname: Hibino
authors.forename: T
authors.initials: T
authors.lastname: Kato
authors.forename: K
authors.initials: K
authors.lastname: Kawabe
authors.forename: G
authors.initials: G
authors.lastname: Takeba
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: S
authors.initials: S
authors.lastname: Fujii