title: Simultaneous HPLC quantification of five major triterpene alcohol and sterol ferulates in rice bran oil using a single reference standard.
abstract: A high performance liquid chromatography (HPLC) method was developed for simultaneous quantification of five major triterpene alcohol and sterol ferulates in rice bran oils (RBO) with a single internal standard, cycloartenyl ferulate. The five compounds are cycloartenyl ferulate (1), 24-methylene cycloartanyl ferulate (2), campesteryl ferulate (3), sitosteryl ferulate (4) and stigmastanyl ferulate (5). All five compounds had good linear concentration-measurement relationships (r(2) ≥ 0.9995) and possessed similar relative response factors. The relative deviation of this method was less than 2.5% for intra- and inter-day assays, and the average recovery varied from 95.1% to 99.4%. The new method was validated by comparing the amount of 24-methylene cycloartanyl ferulate (2) in 17 RBO samples obtained with this method and that with an external standard method. This method was also successfully applied to determine five major triterpene alcohol and sterol ferulates in 17 batches of RBO samples. The results demonstrated that the present method could be utilised for quality control of RBO since some of the reference standards are not commercially available.
authors.affiliation: Institute of Food and Nutraceutical Science, SJTU-Rich Research Institute of Nutrition and Skin Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Weiying
authors.initials: W
authors.lastname: Lu
authors.forename: Yuge
authors.initials: Y
authors.lastname: Niu
authors.forename: Haisha
authors.initials: H
authors.lastname: Yang
authors.forename: Yi
authors.initials: Y
authors.lastname: Sheng
authors.forename: Haiming
authors.initials: H
authors.lastname: Shi
authors.forename: Liangli Lucy
authors.initials: LL
authors.lastname: Yu
mesh_terms: D000438:Alcohols / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D010938:Plant Oils / Q000737:chemistry*; D012015:Reference Standards; D000073879:Rice Bran Oil; D013261:Sterols / Q000032:analysis*; D014315:Triterpenes / Q000032:analysis*
chemical_list: D000438:Alcohols; D010938:Plant Oils; D013261:Sterols; D014315:Triterpenes; D000073879:Rice Bran Oil
keywords: HPLC; Relative response factors; Rice bran oil; Triterpene alcohol ferulate