title: Production of Nicotiana tabacum x Nicotiana acuminata hybrid by ovule culture.
abstract: Using a conventional sexual crossing technique, Nicotiana tabacum x N. acuminata was not produced. After the fertilized ovules were cultured for 20 days in a liquid Nitsch H medium, germination was observed. The roots grew rapidly but leaves did not. However, plantlets were produced in an H medium containing Benzyladenine or Kinetin (0.01-0.1 mg/l). The plantlets grew and flowered in a greenhouse. The chromosome number of the hybrid was 36 and its morphological characteristics were intermediate between those of parental species.
authors.affiliation: Central Research Institute, Japan Tobacco Inc., 6-2 Umegaoka, Mldori-ku, 227, Yokohama, Kanagawa, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Iwai
authors.forename: C
authors.initials: C
authors.lastname: Kishi
authors.forename: K
authors.initials: K
authors.lastname: Nakata
authors.forename: N
authors.initials: N
authors.lastname: Kawashima