title: A physical map of the short arm of wheat chromosome 1A.
abstract: Bread wheat (Triticum aestivum) has a large and highly repetitive genome which poses major technical challenges for its study. To aid map-based cloning and future genome sequencing projects, we constructed a BAC-based physical map of the short arm of wheat chromosome 1A (1AS). From the assembly of 25,918 high information content (HICF) fingerprints from a 1AS-specific BAC library, 715 physical contigs were produced that cover almost 99% of the estimated size of the chromosome arm. The 3,414 BAC clones constituting the minimum tiling path were end-sequenced. Using a gene microarray containing ∼40 K NCBI UniGene EST clusters, PCR marker screening and BAC end sequences, we arranged 160 physical contigs (97 Mb or 35.3% of the chromosome arm) in a virtual order based on synteny with Brachypodium, rice and sorghum. BAC end sequences and information from microarray hybridisation was used to anchor 3.8 Mbp of Illumina sequences from flow-sorted chromosome 1AS to BAC contigs. Comparison of genetic and synteny-based physical maps indicated that ∼50% of all genetic recombination is confined to 14% of the physical length of the chromosome arm in the distal region. The 1AS physical map provides a framework for future genetic mapping projects as well as the basis for complete sequencing of chromosome arm 1AS.
authors.affiliation: Institute of Plant Biology, University of Zurich, Zurich, Switzerland.
authors.forename: James
authors.initials: J
authors.lastname: Breen
authors.forename: Thomas
authors.initials: T
authors.lastname: Wicker
authors.forename: Margarita
authors.initials: M
authors.lastname: Shatalina
authors.forename: Zeev
authors.initials: Z
authors.lastname: Frenkel
authors.forename: Isabelle
authors.initials: I
authors.lastname: Bertin
authors.forename: Romain
authors.initials: R
authors.lastname: Philippe
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Spielmeyer
authors.forename: Hana
authors.initials: H
authors.lastname: Simková
authors.forename: Jan
authors.initials: J
authors.lastname: Safář
authors.forename: Federica
authors.initials: F
authors.lastname: Cattonaro
authors.forename: Simone
authors.initials: S
authors.lastname: Scalabrin
authors.forename: Federica
authors.initials: F
authors.lastname: Magni
authors.forename: Sonia
authors.initials: S
authors.lastname: Vautrin
authors.forename: Hélène
authors.initials: H
authors.lastname: Bergès
authors.forename: Etienne
authors.initials: E
authors.lastname: Paux
authors.forename: Tzion
authors.initials: T
authors.lastname: Fahima
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Doležel
authors.forename: Abraham
authors.initials: A
authors.lastname: Korol
authors.forename: Catherine
authors.initials: C
authors.lastname: Feuillet
authors.forename: Beat
authors.initials: B
authors.lastname: Keller
mesh_terms: D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant; D005819:Genetic Markers; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
chemical_list: D005819:Genetic Markers