title: tRNA 3' processing in yeast involves tRNase Z, Rex1, and Rrp6.
abstract: Mature tRNA 3' ends in the yeast Saccharomyces cerevisiae are generated by two pathways: endonucleolytic and exonucleolytic. Although two exonucleases, Rex1 and Rrp6, have been shown to be responsible for the exonucleolytic trimming, the identity of the endonuclease has been inferred from other systems but not confirmed in vivo. Here, we show that the yeast tRNA 3' endonuclease tRNase Z, Trz1, is catalyzing endonucleolytic tRNA 3' processing. The majority of analyzed tRNAs utilize both pathways, with a preference for the endonucleolytic one. However, 3'-end processing of precursors with long 3' trailers depends to a greater extent on Trz1. In addition to its function in the nucleus, Trz1 processes the 3' ends of mitochondrial tRNAs, contributing to the general RNA metabolism in this organelle.
authors.forename: Ewa
authors.initials: E
authors.lastname: Skowronek
authors.forename: Pawel
authors.initials: P
authors.lastname: Grzechnik
authors.forename: Bettina
authors.initials: B
authors.lastname: Späth
authors.forename: Anita
authors.initials: A
authors.lastname: Marchfelder
authors.forename: Joanna
authors.initials: J
authors.lastname: Kufel
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism; D004722:Endoribonucleases / Q000502:physiology*; D005095:Exoribonucleases / Q000502:physiology*; D063326:Exosome Multienzyme Ribonuclease Complex / Q000502:physiology*; D020868:Gene Silencing; D053858:Metabolic Networks and Pathways / Q000235:genetics; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D030781:Organisms, Genetically Modified; D012313:RNA / Q000378:metabolism; D026721:RNA 3' End Processing / Q000235:genetics*; D000077278:RNA, Mitochondrial; D012343:RNA, Transfer / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*
chemical_list: D000077278:RNA, Mitochondrial; D029701:Saccharomyces cerevisiae Proteins; D012313:RNA; D012343:RNA, Transfer; D004722:Endoribonucleases; D005095:Exoribonucleases; D063326:Exosome Multienzyme Ribonuclease Complex; C512661:tRNase Z, S cerevisiae; C529500:Rex1 exonuclease, S cerevisiae; C112610:RRP6 protein, S cerevisiae
keywords: 3′-end processing; Saccharomyces cerevisiae; exonucleases; tRNA; tRNase Z