title: Enhanced β-galactosidase production of Aspergillus oryzae mutated by UV and LiCl.
abstract: In order to breed a high-yield β-galactosidase-producing strain, Aspergillus oryzae was used as the parent strain and mutagenized with ultraviolet (UV) and UV plus lithium chloride (LiCl), respectively. After being mutagenized by UV, the β-galactosidase activity of mutant UV-15-20 reached 114.08 U/mL, which revealed a 49.22% increase compared with the original strain. A mutant UV-LiCl-38 with high β-galactosidase activity (121.42 U/mL) was obtained after compound mutagenesis of UV and LiCl; the β-galactosidase activity of this mutant was 58.82% higher than that of the parent strain. Subculture testing indicated that UV-15-20 and UV-LiCl-38 had good hereditary stability and may be ideal strains for the production of β-galactosidase. Additionally, it was demonstrated that compound mutagenesis with UV and LiCl is an effective mutation method for breeding industrially interesting strains.
authors.affiliation: a College of Food Science and Technology Engineering , Gansu Agricultural University , Lanzhou , China.
authors.forename: Weibing
authors.initials: W
authors.lastname: Zhang
authors.forename: Fangning
authors.initials: F
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.forename: Qi
authors.initials: Q
authors.lastname: Liang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Duiyuan
authors.initials: D
authors.lastname: Ai
authors.forename: Zhigang
authors.initials: Z
authors.lastname: An
mesh_terms: D001236:Aspergillus oryzae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000528:radiation effects; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D018021:Lithium Chloride / Q000494:pharmacology; D016296:Mutagenesis / Q000187:drug effects* / Q000528:radiation effects*; D014466:Ultraviolet Rays; D001616:beta-Galactosidase / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001616:beta-Galactosidase; D018021:Lithium Chloride