title: Production and purification of xylooligosaccharides from oil palm empty fruit bunch fibre by a non-isothermal process.
abstract: Oil palm empty fruit bunches (OPEFB) fibre, a by-product generated from non-woody, tropical perennial oil palm crop was evaluated for xylooligosaccharides (XOS) production. Samples of OPEFB fibre were subjected to non-isothermal autohydrolysis treatment using a temperature range from 150 to 220 °C. The highest XOS concentration, 17.6g/L which relayed from solubilisation of 63 g/100 g xylan was achieved at 210 °C and there was a minimum amount of xylose and furfural being produced. The chromatographic purification which was undertaken to purify the oligosaccharide-rich liquor resulted in a product with 74-78% purity, of which 83-85% was XOS with degree of polymerisation (DP) between 5 and 40.
authors.affiliation: Department of Food and Nutritional Sciences, University of Reading, Whiteknights, P.O. Box 226, Reading RG6 6AP, United Kingdom.
authors.forename: Ai Ling
authors.initials: AL
authors.lastname: Ho
authors.affiliation: LNEG, Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
authors.forename: Florbela
authors.initials: F
authors.lastname: Carvalheiro
authors.affiliation: LNEG, Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
authors.forename: Luís C
authors.initials: LC
authors.lastname: Duarte
authors.affiliation: LNEG, Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
authors.forename: Luísa B
authors.initials: LB
authors.lastname: Roseiro
authors.affiliation: Department of Food and Nutritional Sciences, University of Reading, Whiteknights, P.O. Box 226, Reading RG6 6AP, United Kingdom.
authors.forename: Dimitris
authors.initials: D
authors.lastname: Charalampopoulos
authors.affiliation: Department of Food and Nutritional Sciences, University of Reading, Whiteknights, P.O. Box 226, Reading RG6 6AP, United Kingdom. Electronic address: r.a.rastall@reading.ac.uk.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Rastall
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002850:Chromatography, Gel; D005612:Freeze Drying; D005638:Fruit / Q000737:chemistry*; D005965:Glucuronates / Q000096:biosynthesis / Q000302:isolation & purification*; D006868:Hydrolysis; D009844:Oligosaccharides / Q000096:biosynthesis / Q000302:isolation & purification*; D000073878:Palm Oil; D010938:Plant Oils / Q000737:chemistry*; D058105:Polymerization; D013696:Temperature
chemical_list: D005965:Glucuronates; D009844:Oligosaccharides; D010938:Plant Oils; C570991:xylooligosaccharide; D000073878:Palm Oil
keywords: Autohydrolysis; Gel filtration chromatography; Oil palm empty fruit bunch; Xylooligosaccharides