title: The extract of Elaeocarpus sylvestris inhibits human cytomegalovirus immediate early gene expression and replication in vitro.
abstract: Human cytomegalovirus (HCMV) is a major cause of morbidity and mortality in newborn infants, immunocompromised individuals with HIV/AIDS and organ transplant recipients. In order to identify a novel antiviral candidate for HCMV-related diseases, crude ethanol extracts from plants were screened for their potential inhibitory activity on HCMV replication in vitro. Ethanol (70%) extract of Elaeocarpus sylvestris leaves (ESE) markedly inhibited the replication of the HCMV Towne strain without exhibiting any significant adverse effects on the viability of human foreskin fibroblasts (HFF). In addition, ESE significantly downregulated HCMV immediate early (IE) gene expression. Taken together, this is the first study, to the best of our knowledge, demonstrating that ESE has a potent antiviral activity against HCMV by downregulating HCMV IE gene expression and replication.
authors.affiliation: Department of Life Science, Gachon University, Seongnam-Si, Kyeonggi-Do 461-701, Republic of Korea.
authors.forename: Kim Phuong
authors.initials: KP
authors.lastname: To
authors.affiliation: Department of Life Science, Gachon University, Seongnam-Si, Kyeonggi-Do 461-701, Republic of Korea.
authors.forename: Se Chan
authors.initials: SC
authors.lastname: Kang
authors.affiliation: Department of Life Science, Gachon University, Seongnam-Si, Kyeonggi-Do 461-701, Republic of Korea.
authors.forename: Yoon-Jae
authors.initials: YJ
authors.lastname: Song
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000187:drug effects* / Q000472:pathogenicity; D045467:Elaeocarpaceae / Q000737:chemistry; D005347:Fibroblasts / Q000187:drug effects; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D006801:Humans; D017874:Immediate-Early Proteins / Q000187:drug effects / Q000235:genetics; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D017874:Immediate-Early Proteins; D010936:Plant Extracts