title: Flanking sequence determination and specific PCR identification of transgenic wheat B102-1-2.
abstract: The exogenous fragment sequence and flanking sequence between the exogenous fragment and recombinant chromosome of transgenic wheat B102-1-2 were successfully acquired using genome walking technology. The newly acquired exogenous fragment encoded the full-length sequence of transformed genes with transformed plasmid and corresponding functional genes including ubi, vector pBANF-bar, vector pUbiGUSPlus, vector HSP, reporter vector pUbiGUSPlus, promoter ubiquitin, and coli DH1. A specific polymerase chain reaction (PCR) identification method for transgenic wheat B102-1-2 was established on the basis of designed primers according to flanking sequence. This established specific PCR strategy was validated by using transgenic wheat, transgenic corn, transgenic soybean, transgenic rice, and non-transgenic wheat. A specifically amplified target band was observed only in transgenic wheat B102-1-2. Therefore, this method is characterized by high specificity, high reproducibility, rapid identification, and excellent accuracy for the identification of transgenic wheat B102-1-2.
authors.affiliation: a Liaoning Entry-Exit Inspection and Quarantine Bureau , Dalian , China.
authors.forename: Jijuan
authors.initials: J
authors.lastname: Cao
authors.forename: Junyi
authors.initials: J
authors.lastname: Xu
authors.forename: Tongtong
authors.initials: T
authors.lastname: Zhao
authors.forename: Dongmei
authors.initials: D
authors.lastname: Cao
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.forename: Piqiao
authors.initials: P
authors.lastname: Zhang
authors.forename: Fengxia
authors.initials: F
authors.lastname: Luan
mesh_terms: D001483:Base Sequence; D016386:Chromosome Walking; D018744:DNA, Plant / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods*; D014908:Triticum / Q000235:genetics*
chemical_list: D018744:DNA, Plant