title: Identification of lipid fraction constituents from grasshopper (Chorthippus spp.) abdominal secretion with potential activity in wound healing with the use of GC-MS/MS technique.
abstract: In recent years biologically active compounds isolated from insects call special interest of drug researchers. According to some Polish etnopharmacological observations, secretion from the grasshopper's abdomen (Orthoptera family) is believed to speed up the process of wound healing. In the present work we focused on determination of main components of the lipid fraction of material from grasshopper abdomen using GC-MS/MS. Samples were qualitatively analyzed using gas chromatography coupled with mass spectrometry. Both liquid-liquid extraction and solid-phase extraction pretreatment methods were used to concentrate and fractionate the compounds from the insect. In the derivatized fractions ca. 350 compounds were identified, including substances of known biological activity. The potential agents affecting wound healing have been indicated. A set of compounds characteristic for all the studied Chorthippus spp., have been identified. Data analysis revealed different lipidomic profiles of grasshoppers depending on the insects origin and collection area.
authors.affiliation: Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416, Gdańsk, Poland(1).
authors.forename: Magdalena
authors.initials: M
authors.lastname: Buszewska-Forajta
authors.affiliation: Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416, Gdańsk, Poland(1).
authors.forename: Danuta
authors.initials: D
authors.lastname: Siluk
authors.affiliation: Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416, Gdańsk, Poland(1).
authors.forename: Wiktoria
authors.initials: W
authors.lastname: Struck-Lewicka
authors.affiliation: Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416, Gdańsk, Poland(1).
authors.forename: Joanna
authors.initials: J
authors.lastname: Raczak-Gutknecht
authors.affiliation: Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416, Gdańsk, Poland(1).
authors.forename: Michał J
authors.initials: MJ
authors.lastname: Markuszewski
authors.affiliation: Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416, Gdańsk, Poland(1). Electronic address: roman.kaliszan@gumed.edu.pl.
authors.forename: Roman
authors.initials: R
authors.lastname: Kaliszan
mesh_terms: D000818:Animals; D001685:Biological Factors / Q000737:chemistry* / Q000494:pharmacology*; D002849:Chromatography, Gas / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006110:Grasshoppers / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D059625:Liquid-Liquid Extraction / Q000379:methods; D052616:Solid Phase Extraction / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D001685:Biological Factors; D008055:Lipids
keywords: Chorthippus spp.; GC–MS/MS; Grasshopper's abdominal secretion; Lipidome; Wound healing