title: Purification, antioxidant activity and antiglycation of polysaccharides from Polygonum multiflorum Thunb.
abstract: Polysaccharides, one of the most important constituents in Polygonum multiflorum Thunb, a famous Chinese medicinal herb, were isolated by DEAE-52, Sepharose 4B and Sephacryl S-300 column chromatography. Two polysaccharides (PMP-1 and PMP-2) were identified as homogeneous in molecular weight with HPLC. The molecular weights were 4.8 × 10(2) and 6.1 × 10(2) kDa, respectively. Antioxidant activity tests were performed with two polysaccharides at concentrations of 0.1-1.5mg/mL. The results indicated that the inhibitory activity on oxidation and glycation exhibited a dose-dependent response. PMP-2 exhibited a much stronger antioxidant capacity against free radical, lipid oxidation and protein glycation. The IC₅₀ values of PMP-2 were 0.47, 0.6 and 0.93 mg/mL for superoxide anion scavenging, hydroxyl radical scavenging, and hydroxyl peroxide scavenging, respectively. The inhibitory ability of PMP-2 on lipid oxidation was most markedly in rat liver, followed by heart and kidney. Meanwhile, PMP-2 also showed satisfactory suppression of AGEs formation. This suggested that the polysaccharides present in PM can contribute to the biological effects.
authors.affiliation: Department of Food Science and Technology, Ginling College, Nanjing Normal University, 122# Ninghai Road, Nanjing 210097, China. Electronic address: lishuanglv@126.com.
authors.forename: Lishuang
authors.initials: L
authors.lastname: Lv
authors.forename: Yunhui
authors.initials: Y
authors.lastname: Cheng
authors.forename: Tiesong
authors.initials: T
authors.lastname: Zheng
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Li
authors.forename: Rong
authors.initials: R
authors.lastname: Zhai
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006031:Glycosylation / Q000187:drug effects; D006321:Heart / Q000187:drug effects; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008970:Molecular Weight; D010084:Oxidation-Reduction; D028502:Polygonum / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013481:Superoxides / Q000037:antagonists & inhibitors*
chemical_list: D016166:Free Radical Scavengers; D017127:Glycation End Products, Advanced; D011134:Polysaccharides; D013481:Superoxides; D017665:Hydroxyl Radical
keywords: Antiglycation; Antioxidant; Polygonum multiflorum Thunb; Polysaccharide