title: Experimental and analytical comparative study of optical coefficient of fresh and frozen rat tissues.
abstract: Optical properties of fresh and frozen tissues of rat heart, kidney, brain, liver, and muscle were measured in the 450- to 700-nm range. The total reflectance and transmittance were measured using a well-calibrated integral sphere set-up. Absorption coefficient μa and reduced scattering coefficient μ's were derived from the experimental measurements using the inverse adding doubling technique. The influence of cryogenic processing on optical properties was studied. Interindividual and intraindividual variations were assessed. These new data aim at filling the lack of validated optical properties in the visible range especially in the blue-green region of particular interest for fluorescence and optogenetics preclinical studies. Furthermore, we provide a unique comparison of the optical properties of different organs obtained using the same measurement set-up for fresh and frozen tissues as well as an estimate of the intraindividual and interindividual variability.
authors.affiliation: UMR8165 Université Paris Sud, CNRS Laboratoire Imagerie et Modélisation en Neurobiologie et Cancérologie Paris Sud, Orsay Campus Bâtiment 440, 91405 Orsay, France.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Mesradi
authors.forename: Aurelie
authors.initials: A
authors.lastname: Genoux
authors.forename: Vesna
authors.initials: V
authors.lastname: Cuplov
authors.forename: Darine
authors.initials: D
authors.lastname: Abi Haidar
authors.forename: Sebastien
authors.initials: S
authors.lastname: Jan
authors.forename: Irene
authors.initials: I
authors.lastname: Buvat
authors.forename: Frederic
authors.initials: F
authors.lastname: Pain
mesh_terms: D000042:Absorption; D000818:Animals; D001923:Brain Chemistry / Q000502:physiology; D003198:Computer Simulation; D015925:Cryopreservation; D007668:Kidney / Q000737:chemistry; D008954:Models, Biological; D009010:Monte Carlo Method; D009132:Muscles / Q000737:chemistry; D055620:Optical Phenomena; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012542:Scattering, Radiation