title: Antibacterial activity of nanofibrous mats coated with lysozyme-layered silicate composites via electrospraying.
abstract: A mixture of positively charged lysozyme (LY) and rectorite (REC) composites was electrosprayed onto negatively charged electrospun cellulose acetate (CA) nanofibrous mats. The morphology and average diameter of CA mats and the mats coated with LY-REC were investigated by scanning electron microscopy. The composite mats were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and energy-dispersive X-ray spectroscopy, and the results confirmed that LY and REC were successfully immobilized on the surface of CA mats via electrospraying technique. The small-angle X-ray diffraction results showed that the silicate layers of REC were completely exfoliated. The enzyme activity and bacterial inhibition analysis verified that the antimicrobial effect of the composite fibrous mats was enhanced with the addition of REC. The protein delivery properties and the bound enzyme activity after removal of unbound lysozyme from fibers were measured and showed that the electrospraying technique was suitable for enzyme immobilization.
authors.affiliation: College of Food Science and Technology and the MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, No. 1 Shizishan Road, Wuhan 430070, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Xueyong
authors.initials: X
authors.lastname: Li
authors.forename: Qun
authors.initials: Q
authors.lastname: Wang
authors.forename: Yijun
authors.initials: Y
authors.lastname: Pan
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.forename: Hanqing
authors.initials: H
authors.lastname: Wang
authors.forename: Rong
authors.initials: R
authors.lastname: Song
authors.forename: Hongbing
authors.initials: H
authors.lastname: Deng
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry; D055664:Electrochemical Techniques; D004800:Enzymes, Immobilized / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D008903:Minerals / Q000737:chemistry; D009113:Muramidase / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D056951:Photoelectron Spectroscopy; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000538:Aluminum Silicates; D000900:Anti-Bacterial Agents; D004800:Enzymes, Immobilized; D008903:Minerals; C546376:rectorite; C005062:acetylcellulose; D002482:Cellulose; D009113:Muramidase
keywords: Antibacterial activity; Electrospray; Lysozyme; Nanofibrous mats; Rectorite