title: Oral administration of soluble β-glucan preparation from the cauliflower mushroom, Sparassis crispa (Higher Basidiomycetes) modulated cytokine production in mice.
abstract: Soluble β-glucan preparation from the cold NaOH extract of Sparassis crispa (SCG) is a six-branched 1,3-β-D-glucan that is a major cell-wall structural component in fungi. Leukocytes from DBA/2 mice are highly sensitive to SCG, producing cytokines in vitro. We previously reported that the intraperitoneal (i.p.) administration of β-glucan decreased cytokine induction by SCG in vitro in DBA/2 mice. In this study, we examined the effects of the oral (p.o.) administration of polysaccharide fractions extracted from S. crispa, using hot water (SCHWE), a β-glucan from S. crispa, to DBA/2 mice on cytokine induction by SCG in the spleen in vitro. The level of induction of IFN-γ and GM-CSF by SCG was significantly increased in SCHWE-treated mice. This activity was more clearly observed when chlorpromazine was administered as a pretreatment in SCHWE-treated mice. The production of GM-CSF, IFN-γ, and IL-6 by immune cells in Peyer's patches was higher in SCHWE-treated mice than in control mice. These results suggest that orally administered β-glucan may modulate cytokine induction by SCG in the spleen through the activation of Peyer's patches.
authors.affiliation: Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy & Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
authors.forename: Toshie H
authors.initials: TH
authors.lastname: Hida
authors.forename: Hiromi
authors.initials: H
authors.lastname: Kawaminami
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Ishibashi
authors.forename: Noriko N
authors.initials: NN
authors.lastname: Miura
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Adachi
authors.forename: Naohito
authors.initials: N
authors.lastname: Ohno
mesh_terms: D000284:Administration, Oral; D000363:Agaricales; D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D001688:Biological Products / Q000008:administration & dosage* / Q000494:pharmacology; D002473:Cell Wall / Q000737:chemistry; D016207:Cytokines / Q000378:metabolism*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D007155:Immunologic Factors / Q000008:administration & dosage* / Q000494:pharmacology; D007371:Interferon-gamma / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D010581:Peyer's Patches / Q000187:drug effects / Q000378:metabolism; D013154:Spleen / Q000187:drug effects* / Q000378:metabolism; D047071:beta-Glucans / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D001688:Biological Products; D016207:Cytokines; D007155:Immunologic Factors; D015850:Interleukin-6; D047071:beta-Glucans; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor