title: [Cytogenetic analysis of choroidal melanoma].
abstract: OBJECTIVE
  To investigate the presence of known cytogenetic alterations of choroidal melanoma in a series of patients diagnosed and treated in our Ocular Oncology Service. A review of the present literature on this topic is also presented.
  
  
  METHODS
  Microsatellite analysis (MSA) studies on loss of heterozygosity (LOH) of chromosome 3, as well as multiplex ligation prove amplification (MLPA) on chromosomes 1, 3, 6 and 8, were performed on enucleation or local resection samples obtained from a total of 27 patients, over a 2 year period.
  
  
  RESULTS
  Twenty patients showed at least one of the cytogenetic alterations looked for. A total of 11 cases were found that showed LOH of chromosome 3 (44%), 8 gains of chromosome 8 (30%), 8 gains of chromosome 6p (30%), and 7 partial or total losses of chromosome 1 (26%).
  
  
  CONCLUSIONS
  This is the first study on the cytogenetics of choroidal melanoma performed in our country. The results are similar to that published in the literature. Cytogenetic analysis provides more accurate knowledge on a vital individual prognosis. It also may become a valuable tool for establishing the most adequate follow-up regimes, and the need for adjuvant therapies.
authors.affiliation: Servicio de Oftalmología, Hospital Universitario de Bellvitge-IDIBELL, Universidad de Barcelona, Hospitalet de Llobregat, Barcelona, España. Electronic address: alejandrofilloy@gmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Filloy
authors.affiliation: Servicio de Oftalmología, Hospital Universitario de Bellvitge-IDIBELL, Universidad de Barcelona, Hospitalet de Llobregat, Barcelona, España.
authors.forename: J M
authors.initials: JM
authors.lastname: Caminal
authors.affiliation: Servicio de Anatomía Patológica, Hospital Universitario de Bellvitge-IDIBELL, Universidad de Barcelona, Hospitalet de Llobregat, Barcelona, España.
authors.forename: M M
authors.initials: MM
authors.lastname: Varela
authors.affiliation: Servicio de Anatomía Patológica, Hospital Universitario de Bellvitge-IDIBELL, Universidad de Barcelona, Hospitalet de Llobregat, Barcelona, España.
authors.forename: M
authors.initials: M
authors.lastname: Gomà
authors.affiliation: Servicio de Oftalmología, Hospital Universitario de Bellvitge-IDIBELL, Universidad de Barcelona, Hospitalet de Llobregat, Barcelona, España.
authors.forename: L
authors.initials: L
authors.lastname: Arias
authors.affiliation: Servicio de Oftalmología, Hospital Universitario de Bellvitge-IDIBELL, Universidad de Barcelona, Hospitalet de Llobregat, Barcelona, España.
authors.forename: J
authors.initials: J
authors.lastname: Arruga
mesh_terms: D000368:Aged; D000782:Aneuploidy; D001918:Brachytherapy; D002830:Choroid Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000648:ultrastructure; D015353:Eye Enucleation; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D008545:Melanoma / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D018895:Microsatellite Repeats; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction; D011379:Prognosis; D047368:Tumor Burden
keywords: Análisis de microsatélites; Citogenético; Cytogenetic; Loss of heterozigosity; Melanoma; Micsosatellite analysis; Multiplex-ligation-prove amplification; Pérdida de heterocigosidad; Uveal