title: Lipidomics analysis revealed the phospholipid compositional changes in muscle by chronic exercise and high-fat diet.
abstract: Although it is clear that lipids are responsible for insulin resistance, it is poorly understood what types of lipids are involved. In this study, we verified the characteristic lipid species in skeletal muscle of a chronic exercise training model and a high-fat induced-obesity model. Three different lipidomics analyses revealed phospholipid qualitative changes. As a result, linoleic acid-containing phosphatidylcholine and sphingomyelin and docosahexanoic acid-containing phosphatidylcholine were characterized as chronic exercise training-induced lipids. On the contrary, arachidonic acid-containing phosphatidylcholines, phosphatidylethanolamines, and phosphatidylinositol were characterized as high-fat diet-induced lipids. In addition, minor sphingomyelin, which has long-chain fatty acids, was identified as a high-fat diet-specific lipid. This is the first report to reveal compositional changes in phospholipid molecular species in chronic exercise and high-fat-diet-induced insulin-resistant models. Due to their influence on cell permeability and receptor stability at the cell membrane, these molecules may contribute to the mechanisms underlying insulin sensitivity and several metabolic disorders.
authors.affiliation: 1] Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 1-1, Minami-Osawa, Hachioji, Tokyo, 192-0397, Japan [2] Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, 1-20-1, Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Goto-Inoue
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Yamada
authors.forename: Akiko
authors.initials: A
authors.lastname: Inagaki
authors.forename: Yasuro
authors.initials: Y
authors.lastname: Furuichi
authors.forename: Shinya
authors.initials: S
authors.lastname: Ogino
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Manabe
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Setou
authors.forename: Nobuharu L
authors.initials: NL
authors.lastname: Fujii
mesh_terms: D000818:Animals; D059305:Diet, High-Fat; D005951:Glucose Tolerance Test; D050356:Lipid Metabolism; D008297:Male; D055432:Metabolomics / Q000379:methods; D018482:Muscle, Skeletal / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism*; D010805:Physical Conditioning, Animal; D051381:Rats
chemical_list: D010743:Phospholipids