title: Cell division cycle-associated protein 1 overexpression is essential for the malignant potential of colorectal cancers.
abstract: To identify new cancer biomarkers and therapeutic targets for colorectal cancers (CRCs), we performed immunohistochemical analysis using tissue microarrays covering archival tumor tissue samples from 434 CRC patients and antibodies to cell division cycle-associated protein 1 (CDCA1) that was originally identified as an oncoantigen by our gene expression profile database, and compared its expression with several clinicopathological factors. Strong CDCA1 positivity was associated with poorer prognosis for patients with CRC (P=0.019) and multivariate analysis confirmed its independent prognostic value. In addition, transfection of siRNAs against CDCA1 suppressed its expression and induced apoptosis of CRC cells. These results suggest that CDCA1 could be a prognostic biomarker and a potential therapeutic target for CRCs.
authors.affiliation: Department of Medical Oncology, Shiga University of Medical Science, Otsu, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Atsushi
authors.initials: A
authors.lastname: Takano
authors.forename: Yohei
authors.initials: Y
authors.lastname: Miyagi
authors.forename: Eiju
authors.initials: E
authors.lastname: Tsuchiya
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Sonoda
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Shimizu
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Okabe
authors.forename: Toru
authors.initials: T
authors.lastname: Tani
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Fujiyama
authors.forename: Yataro
authors.initials: Y
authors.lastname: Daigo
mesh_terms: D000368:Aged; D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000276:immunology; D017209:Apoptosis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000276:immunology; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D034741:RNA, Small Interfering; D046888:Tissue Array Analysis
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; C490197:NUF2 protein, human; D034741:RNA, Small Interfering