title: Colostral antibody-mediated and cell-mediated immunity contributes to innate and antigen-specific immunity in piglets.
abstract: Immunoglobulins and immune cells are critical components of colostral immunity; however, their transfer to and function in the neonate, especially maternal lymphocytes, is unclear. Cell-mediated and antibody-mediated immunity in sow blood and colostrum and piglet blood before (PS) and after (AS) suckling were assessed to investigate transfer and function of maternal immunity in the piglet. CD4, CD8, and γδ lymphocytes were found in sow blood and colostrum and piglet blood PS and AS; each had a unique T lymphocyte profile. Immunoglobulins were detected in sow blood, colostrum, and in piglet blood AS; the immunoglobulin profile of piglet serum AS mimicked that of sow serum. These results suggest selectivity in lymphocyte concentration into colostrum and subsequent lymphocyte transfer into the neonate, but that immunoglobulin transfer is unimpeded. Assessment of colostral natural killer activity and antigen-specific proliferation revealed that colostral cells are capable of influencing the innate and specific immune response of neonatal pigs.
authors.affiliation: Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, United States. Electronic address: bandrick@umn.edu.
authors.forename: Meggan
authors.initials: M
authors.lastname: Bandrick
authors.affiliation: Animal Science, College of Veterinary Medicine, Southern Research and Outreach Center, University of Minnesota, Waseca, MN 56093, United States. Electronic address: cariza@corpoica.org.co.
authors.forename: Claudia
authors.initials: C
authors.lastname: Ariza-Nieto
authors.affiliation: Animal Science, College of Veterinary Medicine, Southern Research and Outreach Center, University of Minnesota, Waseca, MN 56093, United States. Electronic address: skbaidoo@umn.edu.
authors.forename: Samuel K
authors.initials: SK
authors.lastname: Baidoo
authors.affiliation: Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, United States. Electronic address: molit001@umn.edu.
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Molitor
mesh_terms: D056704:Adaptive Immunity; D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling; D000941:Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes; D049109:Cell Proliferation; D002478:Cells, Cultured; D003126:Colostrum / Q000276:immunology*; D005260:Female; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007113:Immunity, Innate; D007112:Immunity, Maternally-Acquired; D007136:Immunoglobulins / Q000378:metabolism*; D007694:Killer Cells, Natural / Q000276:immunology*; D011247:Pregnancy; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism; D013552:Swine / Q000276:immunology*
chemical_list: D000941:Antigens; D007136:Immunoglobulins; D016692:Receptors, Antigen, T-Cell, gamma-delta
keywords: 7-aminoactinomycin D; 7AAD; AMI; APC; AS; CFSE; CMC; CMI; Colostrum; ELISA; IgA; IgG; M. hyopneumoniae; Maternally derived immunity; Mycoplasma hyopneumoniae; NK; Neonate; PBMC; PS; Passive transfer; S:P ratio; Swine; T lymphocyte; after-suckling; antibody-mediated immunity; antigen presenting cell; carboxyfluorescein diacetate succinimidyl ester; cell-mediated immunity; colostral mononuclear cells; conA; concanavalin A; enzyme linked immunosorbant assay; immunoglobulin A; immunoglobulin G; natural killer; peripheral blood mononuclear cell; pre-suckling; sample to positive ratio