title: Identification of a Proteus penneri isolate as the causal agent of red body disease of the cultured white shrimp Penaeus vannamei and its control with Bdellovibrio bacteriovorus.
abstract: Bacteriosis has become a major economic problem in the farming of the Pacific white shrimp Penaeus vannamei. However, no definitive data are available about Proteus penneri infection in cultured P. vannamei and its control. In this study, a virulent strain NC was isolated from diseased P. vannamei suffering from red body disease and identified as a P. penneri isolate through phylogenetic analysis and ATB 32GN system. A phylogenetic constructed tree using the neighbour-joining method identified the NC isolate as a P. penneri strain. In addition, Bdellovibrio bacteriovorus conferred significant protection against P. penneri: it exhibited significant bacteriolytic effects on the pathogenic P. penneri, had a wide prey range towards Proteus pathogens, and displayed a good protective efficacy on experimental P. penneri infection in P. vannamei. To the best of our knowledge, this is the first report of farmed P. vannamei infected with P. penneri and its control with B. bacteriovorus.
authors.affiliation: Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture of P. R. China, Shanghai Engineering Research Center of Aquaculture, Shanghai University Knowledge Service Platform, Shanghai Ocean University Aquatic Animal Breeding Center (ZF1206), National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai, 201306, People's Republic of China, hpcao@shou.edu.cn.
authors.forename: Haipeng
authors.initials: H
authors.lastname: Cao
authors.forename: Shan
authors.initials: S
authors.lastname: He
authors.forename: Liqun
authors.initials: L
authors.lastname: Lu
authors.forename: Xianle
authors.initials: X
authors.lastname: Yang
authors.forename: Baiyao
authors.initials: B
authors.lastname: Chen
mesh_terms: D000818:Animals; D000898:Antibiosis; D015373:Bacterial Typing Techniques; D001433:Bacteriolysis; D001501:Bdellovibrio / Q000254:growth & development / Q000502:physiology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000382:microbiology*; D010572:Pest Control, Biological / Q000379:methods; D010802:Phylogeny; D044095:Proteus penneri / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification* / Q000502:physiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S