title: Comparative evaluation of the biological properties of fibrin for bone regeneration.
abstract: Fibrin is a natural provisional matrix found in wound healing, while type I collagen is a major organic component of bone matrix. Despite the frequent use of fibrin and type I collagen in bone regenerative approaches, their comparative efficacies have not yet been evaluated. In the present study, we compared the effects of fibrin and collagen on the proliferation and differentiation of osteoblasts and protein adsorption. Compared to collagen, fibrin adsorbed approximately 6.7 times more serum fibronectin. Moreover, fibrin allowed the proliferation of larger MC3T3-E1 pre-osteoblasts, especially at a low cell density. Fibrin promoted osteoblast differentiation at higher levels than collagen, as confirmed by Runx2 expression and transcriptional activity, alkaline phosphatase activity, and calcium deposition. The results of the present study suggest that fibrin is superior to collagen in the support of bone regeneration.
authors.affiliation: Department of Molecular Genetics, Dental Research Institute, School of Dentistry, Seoul National University, Seoul 110-749, Korea.
authors.forename: Joung-Hwan
authors.initials: JH
authors.lastname: Oh
authors.affiliation: Department of Molecular Genetics, Dental Research Institute, School of Dentistry, Seoul National University, Seoul 110-749, Korea.
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Periodontology, School of Dentistry, Seoul National University, Seoul 110-749, Korea.
authors.forename: Tae-Il
authors.initials: TI
authors.lastname: Kim
authors.affiliation: Department of Molecular Genetics, Dental Research Institute, School of Dentistry, Seoul National University, Seoul 110-749, Korea; Department of Dental Pharmacology & Therapeutics, School of Dentistry, Seoul National University, Seoul 110-749, Korea.
authors.forename: Kyung Mi
authors.initials: KM
authors.lastname: Woo
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D024042:Collagen Type I / Q000494:pharmacology*; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D005337:Fibrin / Q000494:pharmacology*; D051379:Mice
chemical_list: D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; C493716:Runx2 protein, mouse; D005337:Fibrin; D000469:Alkaline Phosphatase; D002118:Calcium