title: Targeted nasal vaccination provides antibody-independent protection against Staphylococcus aureus.
abstract: Despite showing promise in preclinical models, anti-Staphylococcus aureus vaccines have failed in clinical trials. To date, approaches have focused on neutralizing/opsonizing antibodies; however, vaccines exclusively inducing cellular immunity have not been studied to formally test whether a cellular-only response can protect against infection. We demonstrate that nasal vaccination with targeted nanoparticles loaded with Staphylococcus aureus antigen protects against acute systemic S. aureus infection in the absence of any antigen-specific antibodies. These findings can help inform future developments in staphylococcal vaccine development and studies into the requirements for protective immunity against S. aureus.
authors.affiliation: Adjuvant Research Group.
authors.forename: Karen
authors.initials: K
authors.lastname: Misstear
authors.forename: Edel A
authors.initials: EA
authors.lastname: McNeela
authors.forename: Alison G
authors.initials: AG
authors.lastname: Murphy
authors.forename: Joan A
authors.initials: JA
authors.lastname: Geoghegan
authors.forename: Kate M
authors.initials: KM
authors.lastname: O'Keeffe
authors.forename: John
authors.initials: J
authors.lastname: Fox
authors.forename: Kin
authors.initials: K
authors.lastname: Chan
authors.forename: Simon
authors.initials: S
authors.lastname: Heuking
authors.forename: Nicolas
authors.initials: N
authors.lastname: Collin
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Foster
authors.forename: Rachel M
authors.initials: RM
authors.lastname: McLoughlin
authors.forename: Ed C
authors.initials: EC
authors.lastname: Lavelle
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D058491:Bacterial Load / Q000276:immunology; D005260:Female; D007111:Immunity, Cellular / Q000187:drug effects / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000008:administration & dosage; D013203:Staphylococcal Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D013209:Staphylococcal Vaccines / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D007074:Immunoglobulin G; D013209:Staphylococcal Vaccines
keywords: Staphylococcus aureus; adjuvant; cellular immunity; mucosal; nanoparticle; vaccine