title: Imaging material properties of biological samples with a force feedback microscope.
abstract: Mechanical properties of biological samples have been imaged with a force feedback microscope. Force, force gradient, and dissipation are measured simultaneously and quantitatively, merely knowing the atomic force microscopy cantilever spring constant. Our first results demonstrate that this robust method provides quantitative high resolution force measurements of the interaction. The small oscillation imposed on the cantilever and the small value of its stiffness result in vibrational energies much smaller than the thermal energy, reducing interaction with the sample to a minimum. We show that the observed mechanical properties of the sample depend on the force applied by the tip and consequently on the sample indentation.
authors.affiliation: European Synchrotron Radiation Facility, 6 rue Jules Horowitz BP 220, 38043, Grenoble, CEDEX, France; Université Joseph Fourier BP 53, 38041, Grenoble, CEDEX 9, France.
authors.forename: Luca
authors.initials: L
authors.lastname: Costa
authors.forename: Mario S
authors.initials: MS
authors.lastname: Rodrigues
authors.forename: Emily
authors.initials: E
authors.lastname: Newman
authors.forename: Chloe
authors.initials: C
authors.lastname: Zubieta
authors.forename: Joёl
authors.initials: J
authors.lastname: Chevrier
authors.forename: Fabio
authors.initials: F
authors.lastname: Comin
mesh_terms: D004247:DNA / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods*; D010743:Phospholipids / Q000737:chemistry*
chemical_list: D010743:Phospholipids; D004247:DNA
keywords: DNA, Phospholipids; atomic force microscopy; damping coefficient; force feedback microscopy; local mechanical impedance; proteins; stiffness