title: Impaired spatial memory in mice lacking CD3ζ is associated with altered NMDA and AMPA receptors signaling independent of T-cell deficiency.
abstract: The immunoreceptor-associated protein CD3ζ is known for its role in immunity and has also been implicated in neuronal development and synaptic plasticity. However, the mechanism by which CD3ζ regulates synaptic transmission remains unclear. In this study, we showed that mice lacking CD3ζ exhibited defects in spatial learning and memory as examined by the Barnes maze and object location memory tasks. Given that peripheral T cells have been shown to support cognitive functions and neural plasticity, we generated CD3ζ(-/-) mice in which the peripheral T cells were repopulated to a normal level by syngeneic bone marrow transplantation. Using this approach, we showed that T-cell replenishment in CD3ζ(-/-) mice did not restore spatial memory defects, suggesting that the cognitive deficits in CD3ζ(-/-) mice were most likely mediated through a T-cell-independent mechanism. In support of this idea, we showed that CD3ζ proteins were localized to glutamatergic postsynaptic sites, where they interacted with the NMDAR subunit GluN2A. Loss of CD3ζ in brain decreased GluN2A-PSD95 association and GluN2A synaptic localization. This effect was accompanied by a reduced interaction of GluN2A with the key NMDAR downstream signaling protein calcium/calmodulin-dependent protein kinase II (CaMKII). Using the glycine-induced, NMDA-dependent form of chemical long-term potentiation (LTP) in cultured cortical neurons, we showed that CD3ζ was required for activity-dependent CaMKII autophosphorylation and for the synaptic recruitment of the AMPAR subunit GluA1. Together, these results support the model that the procognitive function of CD3ζ may be mediated through its involvement in the NMDAR downstream signaling pathway leading to CaMKII-dependent LTP induction.
authors.affiliation: INSERM Unité Mixte de Recherche 1064, Institut Transplantation Urologie Nephrologie, Centre Hospitalier Universitaire Nantes, Université de Nantes, 44300 Nantes, France; Facultad de Ciencias Biológicas y Medicina Universidad Andrés Bello, Santiago 8370146, Chile; Unité de Formation et de Recherche Sciences et Techniques, Université de Nantes, 44300 Nantes, France; Cellular and Tissular Imaging Core Facility, Université de Nantes (MicroPICell) IFR26, 44300 Nantes, France; and INSERM Unité Mixte de Recherche 913, L'Institut des Maladies de l'Appareil Digestif, Université de Nantes, 44035 Nantes, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Louveau
authors.forename: Julie
authors.initials: J
authors.lastname: Angibaud
authors.forename: Fabienne
authors.initials: F
authors.lastname: Haspot
authors.forename: Maria Cecilia
authors.initials: MC
authors.lastname: Opazo
authors.forename: Reynald
authors.initials: R
authors.lastname: Thinard
authors.forename: Virginie
authors.initials: V
authors.lastname: Thepenier
authors.forename: Stéphane J
authors.initials: SJ
authors.lastname: Baudouin
authors.forename: Laurent
authors.initials: L
authors.lastname: Lescaudron
authors.forename: Philippe
authors.initials: P
authors.lastname: Hulin
authors.forename: Claudia A
authors.initials: CA
authors.lastname: Riedel
authors.forename: Hélène
authors.initials: H
authors.lastname: Boudin
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D017252:CD3 Complex / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D004195:Disease Models, Animal; D004622:Embryo, Mammalian; D005786:Gene Expression Regulation / Q000235:genetics; D005998:Glycine / Q000494:pharmacology; D017493:Leukocyte Common Antigens / Q000235:genetics; D018782:Maze Learning; D008569:Memory Disorders / Q000235:genetics* / Q000503:physiopathology / Q000601:surgery; D008570:Memory, Short-Term / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D018091:Receptors, AMPA / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D021641:Recognition, Psychology / Q000502:physiology; D013601:T-Lymphocytes / Q000473:pathology*
chemical_list: D017252:CD3 Complex; D005998:Glycine; D017493:Leukocyte Common Antigens; D018091:Receptors, AMPA; D016194:Receptors, N-Methyl-D-Aspartate; C121704:CD3 antigen, zeta chain