title: Nitric oxide and tumor necrosis factor-alpha inhibitory substances from the rhizomes of Kaempferia marginata.
abstract: The ethanol extract of the rhizomes of Kaempferia marginata showed a potent inhibitory effect against lipopolysaccharide (LPS)-induced nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha) release in RAW264.7 cells. Moreover, the partition with various organic solvents also inhibited NO production. One new pimarane-type diterpene, 1alpha-acetoxysandaracopimaradien-2alpha-ol (5), along with four known diterpenes (1-4), were isolated from the n-hexane and chloroform layers, respectively. Among these metabolites, compounds 1 and 4 were isolated for the first time from K. marginata. Compounds 1-5 showed significant inhibitory effects on NO production, with IC50 values ranging from 38.6 to 51.9 microM. Furthermore, compound 2 also exhibited significant activity against TNF-alpha release (IC50 = 48.3 microM). These findings may support the use of K. marginata by traditional doctors for treatment of inflammatory-related diseases.
authors.affiliation: Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand.
authors.forename: Kanidta
authors.initials: K
authors.lastname: Kaewkroek
authors.forename: Chatchai
authors.initials: C
authors.lastname: Wattanapiromsakul
authors.forename: Palangpon
authors.initials: P
authors.lastname: Kongsaeree
authors.forename: Supinya
authors.initials: S
authors.lastname: Tewtrakul
mesh_terms: D000818:Animals; D005260:Female; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors*; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity; D010946:Plants, Medicinal / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors*; D030521:Zingiberaceae / Q000737:chemistry* / Q000633:toxicity
chemical_list: D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide