title: Novel histopathological and molecular effects of natural compound pellitorine on larval midgut epithelium and anal gills of Aedes aegypti.
abstract: The yellow fever mosquito, Aedes aegypti, is a vector for transmitting dengue fever and yellow fever. In this study, we assessed the histopathological and molecular effects of pellitorine, an isobutylamide alkaloid, on the third instar of Ae. aegypti larvae. At 5 mg/l concentration of pellitorine, the whole body of the treated larvae became dark in color, particularly damaged thorax and abdominal regions. Pellitorine was targeted mainly on midgut epithelium and anal gills, indicating variably dramatic degenerative responses of the midgut through a sequential epithelial disorganization. The anterior and posterior midgut was entirely necrosed, bearing only gut lumen residues inside the peritrophic membranes. Pellitorine caused comprehensive damage of anal gill cells and branches of tracheole and debris was found in hemolymph of the anal gills. RT-PCR analysis indicates that the compound inhibited gene expression encoding V-type H(+)-ATPase and aquaporine 4 after treatment with 2.21 mg/l pellitorine. These results verify that pellitorine merits further study as a potential larvicide with a specific target site and a lead molecule for the control of mosquito populations.
authors.affiliation: Research Institute for Agriculture and Life Science, Seoul National University, Seoul, Republic of Korea ; WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
authors.forename: Haribalan
authors.initials: H
authors.lastname: Perumalsamy
authors.forename: Jun-Ran
authors.initials: JR
authors.lastname: Kim
authors.forename: Sang Mi
authors.initials: SM
authors.lastname: Oh
authors.forename: Je Won
authors.initials: JW
authors.lastname: Jung
authors.forename: Young-Joon
authors.initials: YJ
authors.lastname: Ahn
authors.forename: Hyung Wook
authors.initials: HW
authors.lastname: Kwon
mesh_terms: D000330:Aedes / Q000187:drug effects* / Q000378:metabolism; D001003:Anal Canal / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D020346:Aquaporins / Q000378:metabolism; D004064:Digestive System / Q000187:drug effects* / Q000378:metabolism; D004848:Epithelium / Q000187:drug effects* / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000502:physiology*; D015870:Gene Expression / Q000187:drug effects; D005880:Gills / Q000187:drug effects* / Q000378:metabolism; D006458:Hemolymph / Q000187:drug effects / Q000378:metabolism; D007814:Larva / Q000187:drug effects* / Q000378:metabolism; D053284:Polyunsaturated Alkamides; D025262:Vacuolar Proton-Translocating ATPases / Q000378:metabolism
chemical_list: D020346:Aquaporins; D005231:Fatty Acids, Unsaturated; D053284:Polyunsaturated Alkamides; C008778:pellitorine; D025262:Vacuolar Proton-Translocating ATPases