title: A new and improved method based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for the determination of A1298C mutation in the methylenetetrahydrofolate reductase (MTHFR) gene.
abstract: Intracellular folate homeostasis and metabolism is regulated by numerous genes. Among them, 5,10-methylenetetrahydrofolate reductase (MTHFR) is of special interest because of its involvement in regulation of the homocysteine level in the body as a result of folate metabolism. Moreover, some studies demonstrated that the homocysteine plasma level in individuals may be influenced by polymorphisms present in the MTHFR gene. Two common, clinically relevant mutations have been described: MTHFR C677T and MTHFR A1298C. Although several laboratory techniques allow genotyping of both polymorphisms, PCR-RFLP analysis is simple to perform, relatively cheap, and thus one of the most utilized. In the case of A1298C, the PCR-RFLP technique that utilizes MboII endonuclease class II requires an acrylamide gel electrophoresis, since agarose gel electrophoresis is unable to resolve short deoxyribonucleic acid (DNA) fragments after restriction digestion. Agarose gel electrophoresis is commonly preferred over that of acrylamide. To resolve this inconvenience, a novel PCR-RFLP, AjuI-based method to genotype A1298C alleles has been developed that can be performed on standard agarose gel.
authors.affiliation: Department of Pharmacology, Medical University of Silesia, Katowice, Medyków 18, 40-752 Katowice, Poland; phone: +48 32 208 85 17; fax: + 48 32 252 3902; e mail: gmachnik@sum.edu.pl.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Machnik
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Zapala
authors.forename: Ewa
authors.initials: E
authors.lastname: Pelc
authors.forename: Monika
authors.initials: M
authors.lastname: Gasecka-Czapla
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Kaczmarczyk
authors.forename: Boguslaw
authors.initials: B
authors.lastname: Okopien
mesh_terms: D001483:Base Sequence; D005838:Genotype; D006801:Humans; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D020125:Mutation, Missense / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*
chemical_list: D042965:Methylenetetrahydrofolate Reductase (NADPH2)
keywords: Methylenetetrahydrofolate reductase; Polymorphism; molecular biology; mutation; restriction fragment length