title: Systematic genetic analysis of transcription factors to map the fission yeast transcription-regulatory network.
abstract: Mapping transcriptional-regulatory networks requires the identification of target genes, binding specificities and signalling pathways of transcription factors. However, the characterization of each transcription factor sufficiently for deciphering such networks remains laborious. The recent availability of overexpression and deletion strains for almost all of the transcription factor genes in the fission yeast Schizosaccharomyces pombe provides a valuable resource to better investigate transcription factors using systematic genetics. In the present paper, I review and discuss the utility of these strain collections combined with transcriptome profiling and genome-wide chromatin immunoprecipitation to identify the target genes of transcription factors.
authors.affiliation: *Institute for Biocomplexity and Informatics and Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada, T2N 1N4.
authors.forename: Gordon
authors.initials: G
authors.lastname: Chua
mesh_terms: D047369:Chromatin Immunoprecipitation; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D014157:Transcription Factors