title: Antioxidant and immunostimulant β-glucan from edible mushroom Russula albonigra (Krombh.) Fr.
abstract: A water soluble β-glucan (PS) with an average molecular weight ∼1.95 × 10(5)Da was isolated from the alkaline extract of ectomycorrhizal edible mushroom, Russula albonigra (Krombh.) Fr. and found to consist of terminal, (1 → 3)-, (1 → 6)-, and (1 → 3,6)-linked β-D-glucopyranosyl moieties in a ratio of nearly 1:2:2:1. The structure of this PS was elucidated on the basis of total hydrolysis, methylation analysis, Smith degradation, partial hydrolysis, and 1D/2D NMR experiments. On the basis of these experiments, the repeating unit of the PS was found to contain a backbone of three (1 → 6)-β-D-glucopyranosyl residues, one of which was branched at O-3 position with the side chain consisting of two (1 → 3)-β-D-glucopyranosyl and a terminal β-D-glucopyranosyl residue. This PS showed in vitro macrophage activation by NO production as well as splenocytes and thymocytes proliferation. Moreover, it also exhibited potent antioxidant activities.
authors.affiliation: Department of Chemistry & Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Ashis K
authors.initials: AK
authors.lastname: Nandi
authors.forename: Surajit
authors.initials: S
authors.lastname: Samanta
authors.forename: Saikat
authors.initials: S
authors.lastname: Maity
authors.forename: Ipsita K
authors.initials: IK
authors.lastname: Sen
authors.forename: Somanjana
authors.initials: S
authors.lastname: Khatua
authors.forename: K Sanjana P
authors.initials: KS
authors.lastname: Devi
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Acharya
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Maiti
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002240:Carbohydrate Sequence; D049109:Cell Proliferation; D002478:Cells, Cultured; D006868:Hydrolysis; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008262:Macrophage Activation / Q000187:drug effects* / Q000276:immunology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009569:Nitric Oxide / Q000096:biosynthesis; D012995:Solubility; D013481:Superoxides / Q000037:antagonists & inhibitors; D060168:Thymocytes / Q000166:cytology / Q000187:drug effects; D014867:Water; D047071:beta-Glucans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000276:Adjuvants, Immunologic; D000975:Antioxidants; D047071:beta-Glucans; D014867:Water; D013481:Superoxides; D009569:Nitric Oxide; D017665:Hydroxyl Radical
keywords: Antioxidant activities; Immunostimulation; NMR studies; Russula albonigra (Krombh.) Fr.; β-Glucan