title: miR-15a inhibits cell proliferation and epithelial to mesenchymal transition in pancreatic ductal adenocarcinoma by down-regulating Bmi-1 expression.
abstract: OBJECTIVE
  To investigate whether miR-15a inhibits cell proliferation and epithelial-mesenchymal transition (EMT) in pancreatic ductal adenocarcinoma (PDAC) via the down-regulation of B cell-specific moloney murine leukemia virus insertion site 1 (Bmi-1) expression.
  
  
  RESULTS
  miR-15a and Bmi-1 expressions in normal pancreatic tissue and PDAC tissue were measured. The relationship between miR-15a and Bmi-1 expression was analyzed. We found that miR-15a suppressed the expression of Bmi-1 and PDAC cell proliferation; E-cadherin expression was visibly up-regulated after silencing Bmi-1 by transfecting miR-15a into PDAC cell line.
  
  
  CONCLUSIONS
  miR-15a inhibits cell proliferation and EMT in PDAC via the down-regulation of Bmi-1 expression.
authors.affiliation: Institute of Hepatopancreatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Shixiang
authors.initials: S
authors.lastname: Guo
authors.affiliation: Institute of Hepatopancreatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Xu
authors.affiliation: Institute of Hepatopancreatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Yichen
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Institute of Hepatopancreatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Chaobin
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Institute of Hepatopancreatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Hepatopancreatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Ouyang
authors.affiliation: Translational Research Laboratory, Department of Pathology, Stony Brook University, Stony Brook, NY 11794, USA. Electronic address: jingfang.ju@stonybrookmedicine.edu.
authors.forename: Jingfang
authors.initials: J
authors.lastname: Ju
authors.affiliation: Institute of Hepatopancreatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China. Electronic address: bieping@medmail.com.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Bie
authors.affiliation: Institute of Hepatopancreatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China. Electronic address: whuaizhi@gmail.com.
authors.forename: Huaizhi
authors.initials: H
authors.lastname: Wang
mesh_terms: D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000378:metabolism; D063150:Polycomb Repressive Complex 1 / Q000096:biosynthesis*; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: C069077:BMI1 protein, human; C505361:MIRN15 microRNA, human; D035683:MicroRNAs; D063150:Polycomb Repressive Complex 1
keywords: Bmi-1; EMT; Pancreatic ductal adenocarcinoma; Proliferation; miR-15a