title: Plant regeneration from cultured cell-derived protoplasts of Pelargonium aridum, P. x hortorum and P. peltatum.
abstract: Cultured protoplasts from cell suspensions of Pelargonium aridum, P.x hortorum and P. peltatum divided and formed callus. On agar-solidified regenerative medium, such protoplast-derived calli (p-calli) underwent plant regeneration at frequencies approaching 100% for P. aridum and 10% for P.x hortorum. Under similar conditions shoot primordia arose in 5% of P. peltatum p-calli, but these never developed into normal shoots. However, following a liquid-shake culture regime, whole plants were induced in 20% of P. peltatum p-calli. This approach also improved regeneration of P.x hortorum to 60%.
authors.affiliation: Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, University Park, NG7 2RD, Nottingham, UK.
authors.forename: S A
authors.initials: SA
authors.lastname: Yarrow
authors.forename: E C
authors.initials: EC
authors.lastname: Cocking
authors.forename: J B
authors.initials: JB
authors.lastname: Power