title: A quantitative liposome microarray to systematically characterize protein-lipid interactions.
abstract: Lipids have a role in virtually all biological processes, acting as structural elements, scaffolds and signaling molecules, but they are still largely under-represented in known biological networks. Here we describe a liposome microarray-based assay (LiMA), a method that measures protein recruitment to membranes in a quantitative, automated, multiplexed and high-throughput manner.
authors.affiliation: 1] Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany. [2].
authors.forename: Antoine-Emmanuel
authors.initials: AE
authors.lastname: Saliba
authors.affiliation: 1] Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany. [2].
authors.forename: Ivana
authors.initials: I
authors.lastname: Vonkova
authors.affiliation: Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Stefano
authors.initials: S
authors.lastname: Ceschia
authors.affiliation: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.
authors.forename: Greg M
authors.initials: GM
authors.lastname: Findlay
authors.affiliation: Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Kenji
authors.initials: K
authors.lastname: Maeda
authors.affiliation: Advanced Light Microscopy Facility, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Tischer
authors.affiliation: Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Samy
authors.initials: S
authors.lastname: Deghou
authors.affiliation: Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Vera
authors.initials: V
authors.lastname: van Noort
authors.affiliation: Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Peer
authors.initials: P
authors.lastname: Bork
authors.affiliation: 1] Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada. [2].
authors.forename: Tony
authors.initials: T
authors.lastname: Pawson
authors.affiliation: Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Ellenberg
authors.affiliation: Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Anne-Claude
authors.initials: AC
authors.lastname: Gavin
mesh_terms: D001331:Automation; D004926:Escherichia coli / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D046228:Microarray Analysis; D008856:Microscopy, Fluorescence / Q000379:methods; D009154:Mutation; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012685:Sepharose / Q000737:chemistry; D015398:Signal Transduction; D049490:Systems Biology
chemical_list: D008055:Lipids; D008081:Liposomes; D011506:Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D012685:Sepharose