title: Identification of fungal pathogens from clinical specimens using multi-locus PCR coupled with electrospray ionization mass spectrometry.
abstract: Polymerase chain reaction coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was successfully used to identify a variety of fungi, including mixed fungal species, from 10 of 12 clinical specimens (10 culture-negative) of biopsy tissues or body fluids from patients with fungal infections. The application of PCR/ESI-MS for identifying fungal pathogens is discussed.
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Tainan, Taiwan; Department of Internal Medicine, National Cheng Kung University Hospital and Medical College, Tainan, Taiwan. Electronic address: wucj@nhri.org.tw.
authors.forename: Chi-Jung
authors.initials: CJ
authors.lastname: Wu
authors.affiliation: Department of Internal Medicine, National Cheng Kung University Hospital and Medical College, Tainan, Taiwan.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Chen
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Tainan, Taiwan.
authors.forename: Hsuan-Chen
authors.initials: HC
authors.lastname: Wang
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Tainan, Taiwan.
authors.forename: Ih-Jen
authors.initials: IJ
authors.lastname: Su
authors.affiliation: Department of Internal Medicine, National Cheng Kung University Hospital and Medical College, Tainan, Taiwan.
authors.forename: Wen-Chien
authors.initials: WC
authors.lastname: Ko
authors.affiliation: Department of Pediatrics, National Cheng Kung University Hospital and Medical College, Tainan, Taiwan.
authors.forename: Jiann-Shiuh
authors.initials: JS
authors.lastname: Chen
authors.affiliation: Department of Pediatrics, National Cheng Kung University Hospital and Medical College, Tainan, Taiwan.
authors.forename: Chao-Neng
authors.initials: CN
authors.lastname: Cheng
authors.affiliation: Department of Internal Medicine, National Cheng Kung University Hospital and Medical College, Tainan, Taiwan.
authors.forename: Nan-Yao
authors.initials: NY
authors.lastname: Lee
authors.affiliation: Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: H Sunny
authors.initials: HS
authors.lastname: Sun
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Tainan, Taiwan; Department of Pediatrics, National Cheng Kung University Hospital and Medical College, Tainan, Taiwan.
authors.forename: Chia-Yu
authors.initials: CY
authors.lastname: Chi
authors.affiliation: Department of Internal Medicine, National Cheng Kung University Hospital and Medical College, Tainan, Taiwan. Electronic address: teresa@mail.ncku.edu.tw.
authors.forename: Tsai-Yun
authors.initials: TY
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D005658:Fungi / Q000145:classification* / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D058885:Multilocus Sequence Typing; D009181:Mycoses / Q000175:diagnosis / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D055815:Young Adult
keywords: Electrospray ionization mass spectrometry; Fungal infection; PCR; Paraffin