title: Advanced Motion Compensation Methods for Intravital Optical Microscopy.
abstract: Intravital microscopy has emerged in the recent decade as an indispensible imaging modality for the study of the micro-dynamics of biological processes in live animals. Technical advancements in imaging techniques and hardware components, combined with the development of novel targeted probes and new mice models, have enabled us to address long-standing questions in several biology areas such as oncology, cell biology, immunology and neuroscience. As the instrument resolution has increased, physiological motion activities have become a major obstacle that prevents imaging live animals at resolutions analogue to the ones obtained in vitro. Motion compensation techniques aim at reducing this gap and can effectively increase thein vivoresolution. This paper provides a technical review of some of the latest developments in motion compensation methods, providing organ specific solutions.
authors.affiliation: Center for System Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center, 185 Cambridge Street, Boston 02114, USA.
authors.forename: Claudio
authors.initials: C
authors.lastname: Vinegoni
authors.forename: Sungon
authors.initials: S
authors.lastname: Lee
authors.forename: Paolo Fumene
authors.initials: PF
authors.lastname: Feruglio
authors.forename: Ralph
authors.initials: R
authors.lastname: Weissleder
keywords: Intravital microscopy; image stabilization; in vivo imaging; motion artifact and motion compensation