title: Polycystin 2 is involved in the nitric oxide production in responding to oscillating fluid shear in MLO-Y4 cells.
abstract: As a mechano-calcium channel, polycystin2 (PC2) play an important role in the response of renal epithelial cells to fluid flow shear stress. In bone tissue, osteocytes are well known as the main mechanosensory cells, and sensitive to fluid flow stimulus in vitro. In the study, we investigated the effects of oscillating fluid flow (OFF, 2 h, 1 Hz, 1.0 Pa) on the release of Nitric Oxide (NO) and ProstaglandinE2 (PGE2), and the role of PC2 on the release. Our findings demonstrate that PC2 expression increases after 2 h of OFF, and silencing PC2 by RNAi inhibits downstream NO production and iNOS expression, but does not affect the response of PGE2 to OFF.
authors.affiliation: Key Laboratory for Space Biosciences & Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi Xilu, Xi'an 710072, Shaanxi Province, People's Republic of China.
authors.forename: Huiyun
authors.initials: H
authors.lastname: Xu
authors.affiliation: Key Laboratory for Space Biosciences & Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi Xilu, Xi'an 710072, Shaanxi Province, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Guan
authors.affiliation: Key Laboratory for Space Biosciences & Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi Xilu, Xi'an 710072, Shaanxi Province, People's Republic of China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Laboratory for Space Biosciences & Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi Xilu, Xi'an 710072, Shaanxi Province, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Space Biosciences & Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi Xilu, Xi'an 710072, Shaanxi Province, People's Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: Duan
authors.affiliation: Key Laboratory for Space Biosciences & Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi Xilu, Xi'an 710072, Shaanxi Province, People's Republic of China.
authors.forename: Long
authors.initials: L
authors.lastname: An
authors.affiliation: Key Laboratory for Space Biosciences & Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi Xilu, Xi'an 710072, Shaanxi Province, People's Republic of China. Electronic address: celldon@nwpu.edu.cn.
authors.forename: Peng
authors.initials: P
authors.lastname: Shang
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001826:Body Fluids; D001842:Bone and Bones / Q000378:metabolism; D002460:Cell Line; D015232:Dinoprostone / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000378:metabolism / Q000502:physiology*; D010011:Osteocytes / Q000166:cytology*; D012212:Rheology; D013314:Stress, Mechanical; D050396:TRPP Cation Channels / Q000378:metabolism* / Q000502:physiology*
chemical_list: D015232:Dinoprostone; D009569:Nitric Oxide; D050396:TRPP Cation Channels
keywords: Nitric oxide; Oscillating fluid flow; Osteocyte; Polycystin2; ProstaglandinE2