title: An improved method to isolate lichen algae by gel filtration.
abstract: Photobiont cells of the lichen Evernia prunastri have completely been separated from their fungal partner by filtration through a bed of Sepharose 2B. Both mannitol and ribitol have been quantified by gas-liquid chromatography in the different steps of the isolation procedure. Absence of mannitol, which is exclusively produced by the mycobiont, has been used as the best probe to monitor isolation.
authors.affiliation: Department of Plant Physiology, The Lichen Team, Faculty of Biology, Complutense University, E-28040, Madrid, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Pérez
authors.forename: C
authors.initials: C
authors.lastname: Vicente
authors.forename: M E
authors.initials: ME
authors.lastname: Legaz