title: Age-dependent increased expression and activity of inorganic pyrophosphatase in the liver of male mice and its further enhancement with short- and long-term dietary restriction.
abstract: Intracellular orthophosphate and inorganic pyrophosphate (PPi) are by-products of multiple biosynthetic reactions. PPi hydrolysis by soluble inorganic pyrophosphatase (iPPase) has been considered as an important homeostatic mechanism. We investigated the expression and activities (U/mg protein) of iPPase in the liver of young and old mice subjected to short- and long-term dietary restriction. The expression level of iPPase was ascertained by the Western blot analysis using anti-iPPase and differential polymerase chain reaction using iPPase specific primer. Older mice showed a significant increase in the expression and activity of iPPase as compared to younger ones. Short-term fasting of 24 h increased the expression and activity of iPPase in the liver of both young and old mice which were reversed upon 24 h of re-feeding them. However, both young and old mice on long-term dietary restriction showed a cumulative increase in the expression and activity of iPPase when compared with their age-matched controls. This might be due to accumulative adaptation to refill energy deficiency of long-term dietary restricted mice for ATP generation via oxidative phosphorylation, where fatty acid activation could be driven by elevated iPPase.
authors.affiliation: Department of Biochemistry, North-Eastern Hill University, Shillong, 793 022, India.
authors.forename: Wanaiborlang Jala
authors.initials: WJ
authors.lastname: Kharbhih
authors.forename: Ramesh
authors.initials: R
authors.lastname: Sharma
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000375:Aging / Q000378:metabolism*; D000818:Animals; D005215:Fasting / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D006706:Homeostasis / Q000502:physiology; D043564:Inorganic Pyrophosphatase / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D013997:Time Factors
chemical_list: D005227:Fatty Acids; D000255:Adenosine Triphosphate; D043564:Inorganic Pyrophosphatase