title: Caveolin-1 expression in papillary thyroid carcinoma: correlation with clinicopathological parameters and BRAF mutation status.
abstract: OBJECTIVE
  We aimed to investigate the role of caveolin-1 in papillary thyroid carcinoma pathogenesis.
  
  
  METHODS
  Case series with chart review.
  
  
  METHODS
  Institute for the Application of Nuclear Energy.
  
  
  METHODS
  We evaluated the expression of caveolin-1 in papillary thyroid carcinoma (PTC) by Western blot (WB) and compared the findings with immunohistochemical (IHC) expression of both epithelial and stromal caveolin-1 on the corresponding histological specimens. The results were related to clinicopathological features and BRAF mutation status.
  
  
  RESULTS
  Caveolin-1 expression was found in malignant thyroid epithelium and more abundantly in tumor stroma but varied in both compartments within and between PTC subtypes. Caveolin-1 expression in the epithelium was more intense in classical PTC than in the other histological types. On the contrary, stromal caveolin-1 expression was stronger in the follicular, solid, and trabecular PTC variants than in classical PTC. Trends for down-regulation of caveolin-1 expression in epithelium and up-regulation in stroma from the classical via follicular to the solid variant were observed. The relation of WB and IHC results with clinicopathological parameters showed lower caveolin-1 tissue content in BRAF mutated tumors (P < .05), a positive correlation of epithelial caveolin-1 expression with lymph node metastasis (P < .05), and a negative association of stromal caveolin-1 expression with the degree of neoplastic infiltration and BRAF status.
  
  
  CONCLUSIONS
  Altered expression of caveolin-1 in the thyroid epithelial and stromal compartments may be involved in the pathogenesis of PTC. The potential clinical significance of caveolin-1 expression, as well as its relation to BRAF mutation status, deserves further investigation.
authors.affiliation: Institute for the Application of Nuclear Energy-INEP, Department for Endocrinology and Immunoradiology, Belgrade, Serbia.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Paskaš
authors.forename: Jelena
authors.initials: J
authors.lastname: Janković
authors.forename: Ilona
authors.initials: I
authors.lastname: Marečko
authors.forename: Tijana
authors.initials: T
authors.lastname: Išić Denčić
authors.forename: Svetislav
authors.initials: S
authors.lastname: Tatić
authors.forename: Dubravka
authors.initials: D
authors.lastname: Cvejić
authors.forename: Svetlana
authors.initials: S
authors.lastname: Savin
mesh_terms: D000328:Adult; D015153:Blotting, Western; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002291:Carcinoma, Papillary / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051242:Caveolin 1 / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009154:Mutation; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: C495540:CAV1 protein, human; D051242:Caveolin 1; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf
keywords: BRAF; caveolin-1; immunohistochemistry; papillary thyroid cancer