title: 15-Cis-β-carotene found in the reaction center of spinach Photosystem I.
abstract: β-Carotene was extracted from spinach Photosystem I reaction centers (one consisting of the Psa A, B, C, D and E subunits and the other consisting of the Psa A and B subunits alone), and the extract was subjected to high-pressure liquid chromatography using an apparatus equipped with a two-dimensional diode-array detector; all the procedures were performed at ≈ 4 °C in complete darkness. Both 15-cis and all-trans-β-carotene were identified in the extract by means of electronic absorption spectroscopy. Thus, universal presence of 15-cis carotenoid in the reaction centers of purple photosynthetic bacteria and of spinach Photosystem I and Photosystem II has been shown.
authors.affiliation: Faculty of Science, Kwansei Gakuin University, Uegahara, 662, Nishinomiya, Japan.
authors.forename: G E
authors.initials: GE
authors.lastname: Bialek-Bylka
authors.forename: T
authors.initials: T
authors.lastname: Hiyama
authors.forename: K
authors.initials: K
authors.lastname: Yumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Koyama