title: Preparation of glial plasma membrane from a cell fraction enriched in astrocytes.
abstract: A technique for obtaining glial plasma membrane has been developed, starting with a bulk-prepared glial cell-enriched fraction from rabbit cerebral cortex. The astrocytic-enriched fraction was hand-homogenized in isotonic sucrose media, and the crude membrane fraction sedimented at 3,000g. The isolation of a membrane-enriched fraction was accomplished with sucrose density gradient centrifugation. The plasma membrane fraction was collected at the interphase between 31.5% and 25.5% sucrose. Enzymatic and electron-microscopical analyses indicated a 4-7-fold enrichment in plasma membrane, and a 15-20% contamination with microsomal and mitochondrial material. Some multilaminar membrane structures were also seen in the fraction.
authors.affiliation: College of Medicine, University of Iowa, 52242, Iowa City, Iowa.
authors.forename: F A
authors.initials: FA
authors.lastname: Henn
authors.forename: A
authors.initials: A
authors.lastname: Hamberger