title: A novel hairpin-like antimicrobial peptide from barnyard grass (Echinochloa crusgalli L.) seeds: Structure-functional and molecular-genetics characterization.
abstract: A novel plant hairpin-like defense polypeptide named EcAMP3 was isolated from latent barnyard grass (Echinochloa crusgalli L.) seeds. The native peptide and its recombinant analogue were characterized. EcAMP3 displays antifungal and antibacterial activity in vitro. The gene family encoding EcAMPs precursor protein was also characterized; the genes and pseudogenes of this family show 97-100% homology. Every member of EcAMPs precursor family contains seven identical cysteine motifs: C1XXXC2(11-13)C3XXXC4. One of those motifs corresponds to the isolated peptide. EcAMP3 is the first member of the plant hairpin-like peptide family that inhibits the growth of phytopathogenic bacteria. Obtained results can explain the nature of the complex resistance of barnyard grass to a variety of pathogenic microorganisms.
authors.affiliation: Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russian Federation. Electronic address: d.yu.ryazantsev@gmail.com.
authors.forename: Dmitry Yu
authors.initials: DY
authors.lastname: Ryazantsev
authors.affiliation: Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russian Federation. Electronic address: rea21@list.ru.
authors.forename: Eugene A
authors.initials: EA
authors.lastname: Rogozhin
authors.affiliation: Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russian Federation. Electronic address: dimitrieva.ta@gmail.com.
authors.forename: Tatiana V
authors.initials: TV
authors.lastname: Dimitrieva
authors.affiliation: Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russian Federation; All-Russian Research Institute of Biotechnology, Russian Academy of Agricultural Sciences, ul. Timiryazevskaya 42, 127550 Moscow, Russian Federation. Electronic address: polina.drobyazina@gmail.com.
authors.forename: Polina E
authors.initials: PE
authors.lastname: Drobyazina
authors.affiliation: Vavilov Institute of General Genetics, Russian Academy of Sciences, ul. Gubkina 3, 117809 Moscow, Russian Federation. Electronic address: khadeeva@vigg.ru.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Khadeeva
authors.affiliation: Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russian Federation.
authors.forename: Tsezi A
authors.initials: TA
authors.lastname: Egorov
authors.affiliation: Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russian Federation. Electronic address: grev@mx.ibch.ru.
authors.forename: Eugene V
authors.initials: EV
authors.lastname: Grishin
authors.affiliation: Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russian Federation. Electronic address: szavriev@ibch.ru.
authors.forename: Sergey K
authors.initials: SK
authors.lastname: Zavriev
mesh_terms: D000192:Actinomycetales / Q000187:drug effects; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D001234:Aspergillus niger / Q000187:drug effects; D031747:Echinochloa / Q000737:chemistry*; D005670:Fusarium / Q000187:drug effects; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016973:Pectobacterium carotovorum / Q000187:drug effects; D010935:Plant Diseases / Q000382:microbiology; D010936:Plant Extracts / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D057865:Plant Immunity; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D017433:Protein Structure, Secondary; D044224:Pseudomonas syringae / Q000187:drug effects; D012639:Seeds / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D010936:Plant Extracts; D010940:Plant Proteins
keywords: Antibacterial activity; Antifungal activity; Full-length gene structure; Full-size precursor protein; Hairpin-like peptide