title: Erythrocytes' antioxidative capacity as a potential marker of oxidative stress intensity in neuroinflammation.
abstract: The study is designed to assess the oxidative stress intensity in erythrocytes obtained from patients in different clinical phenotypes of neuroinflammation, defined as clinically isolated syndrome (CIS) and relapsing-remitting multiple sclerosis (RRMS). Advanced oxidation protein products (AOPP), malondialdehyde (MDA) and superoxide dismutase (SOD) activity were measured and compared with patients' clinical severity (expanded disability status scale-EDSS), radiological findings (gadolinium enhancement lesion volume-Gd+) and disease duration (DD). AOPP, MDA values and SOD activity were significantly higher in both study patients than in the control group (p < 0.05). While AOPP and MDA approached higher values in RRMS, compared to the CIS group (p > 0.05, p < 0.05, respectively), SOD activity showed higher values in CIS than in RRMS patients (p < 0.05). Both study patients with higher EDSS, higher number of total radiological lesions and longer DD, had higher AOPP and MDA content (p < 0.05, p > 0.05). SOD activity was lower in both study patients with higher EDSS, higher number of total radiological lesions and longer DD (p < 0.05, p > 0.05). There were positive correlations between AOPP and DD and EDSS in CIS patients (p < 0.01), and MDA levels and DD, EDSS and Gd+ in CIS, as well as with EDSS in RRMS patients (p < 0.01). There were negative correlations between SOD activity and DD and EDSS in both study patients (p < 0.01), as well as, between SOD activity and Gd+ in CIS patients (p < 0.01). The measured erythrocytes' biomarkers might represent one of the important biomarkers for the evaluation of the oxidative status of neuroinflammation and disease severity, especially in its early phase, defined as CIS.
authors.affiliation: Clinic of Neurology, Clinical Center Nis, Bul. Dr Zorana Djindjica 48, 18000 Nis, Serbia; Institute for Pathophysiology, Faculty of Medicine, University of Nis, Bul. Dr Zorana Djindjica 81, 18000 Nis, Serbia. Electronic address: srljub@gmail.com.
authors.forename: Srdjan
authors.initials: S
authors.lastname: Ljubisavljevic
authors.affiliation: Institute for Biochemistry, Faculty of Medicine, University of Nis, Bul. Dr Zorana Djindjica 81, 18000 Nis, Serbia.
authors.forename: Ivana
authors.initials: I
authors.lastname: Stojanovic
authors.affiliation: Institute for Biochemistry, Faculty of Medicine, University of Nis, Bul. Dr Zorana Djindjica 81, 18000 Nis, Serbia.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Cvetkovic
authors.affiliation: Clinic of Neurology, Clinical Center Nis, Bul. Dr Zorana Djindjica 48, 18000 Nis, Serbia.
authors.forename: Slobodan
authors.initials: S
authors.lastname: Vojinovic
authors.affiliation: Center for Radiology, Clinical Center Nis, Bul. Dr Zorana Djindjica 48, 18000 Nis, Serbia.
authors.forename: Dragan
authors.initials: D
authors.lastname: Stojanov
authors.affiliation: Institute for Pathophysiology, Faculty of Medicine, University of Nis, Bul. Dr Zorana Djindjica 81, 18000 Nis, Serbia.
authors.forename: Dijana
authors.initials: D
authors.lastname: Stojanovic
authors.affiliation: Department for Pharmacy, Faculty of Medicine, University of Nis, Bul. Dr Zorana Djindjica 81, 18000 Nis, Serbia.
authors.forename: Nikola
authors.initials: N
authors.lastname: Stefanovic
authors.affiliation: Institute for Biochemistry, Faculty of Medicine, University of Nis, Bul. Dr Zorana Djindjica 81, 18000 Nis, Serbia.
authors.forename: Dusica
authors.initials: D
authors.lastname: Pavlovic
mesh_terms: D000293:Adolescent; D000328:Adult; D062107:Advanced Oxidation Protein Products / Q000378:metabolism; D001772:Blood Cell Count; D003430:Cross-Sectional Studies; D004185:Disability Evaluation; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008875:Middle Aged; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000150:complications / Q000473:pathology*; D019636:Neurodegenerative Diseases / Q000150:complications / Q000473:pathology*; D018384:Oxidative Stress / Q000502:physiology*; D013482:Superoxide Dismutase / Q000378:metabolism; D055815:Young Adult
chemical_list: D062107:Advanced Oxidation Protein Products; D008315:Malondialdehyde; D013482:Superoxide Dismutase
keywords: Advanced oxidation protein products; Clinically isolated syndrome; Malondialdehyde; Relapsing–remitting multiple sclerosis; Superoxide dismutase