title: Immunomodulatory activities of alpha-mangostin on peripheral blood mononuclear cells.
abstract: Mangosteen (Garcinia mangostana L.) a tropical fruit, has been used in traditional medicine. A frequently used part of mangosteen is the pericarp, containing a high content of xanthones. alpha-Mangostin, one of the major xanthone derivatives, exhibits a variety of actions, including antimicrobial, antioxidant, cytotoxic and antitumor; however, its function on the immune system is still equivocal. This study aimed to examine the immunomodulatory activities of alpha-mangostin on lymphocyte lineage and cytokine production in human peripheral blood mononuclear cells (PBMCs). The cytotoxic activity of alpha-mangostin was measured by MTT assay. The concentration of alpha-mangostin at 5.55 microg/mL resulted in a 50% survival of PBMCs, which was as potent a cytotoxic activity as that of paclitaxel. After 24 h of PBMCs culture, the percentages of T cells (CD3+), B cells (CD19+) and NK cells (CD3-CD16+CD56+) were not significantly changed by treatment with 1, 2 and 4 microg/mL of alpha-mangostin compared with untreated-PBMCs; in addition, the percentages of these lymphocytes treated with the combination of alpha-mangostin (1, 2 and 4 microg/mL) and the mitogen concanavalin A (ConA) was not significantly different from that of ConA-treated PBMCs. For cytokine secretion, alpha-mangostin (1, 2 and 4 microg/mL) did not significantly induce either proinflammatory cytokines (i.e., TNF-alpha and IL-1beta) or cytokine of adaptive immunity (i.e., IL-2). The combination of alpha-mangostin (1, 2 and 4 microg/mL) and ConA did not significantly alter the relative difference of TNF-alpha and IL-1beta compared with ConA-treated PBMCs; however, these combinations could significantly decrease the relative difference of IL-2 compared with ConA-treated PBMCs. These data indicated that alpha-mangostin was able to inhibit IL-2 release without interfering with human immune cells; therefore, further studies are necessary to investigate its effect on IL-2 production.
authors.affiliation: Department of Microbiology, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Pimolkan
authors.initials: P
authors.lastname: Kasemwattanaroj
authors.forename: Primchanien
authors.initials: P
authors.lastname: Moongkarndi
authors.forename: Kovit
authors.initials: K
authors.lastname: Pattanapanyasat
authors.forename: Supachoke
authors.initials: S
authors.lastname: Mangmool
authors.forename: Ekkarat
authors.initials: E
authors.lastname: Rodpai
authors.forename: Jutima
authors.initials: J
authors.lastname: Samer
authors.forename: Julaporn
authors.initials: J
authors.lastname: Konlata
authors.forename: Kasama
authors.initials: K
authors.lastname: Sukapirom
mesh_terms: D016207:Cytokines / Q000378:metabolism; D029762:Garcinia mangostana / Q000737:chemistry*; D006801:Humans; D007155:Immunologic Factors / Q000032:analysis*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D044004:Xanthones / Q000494:pharmacology*
chemical_list: D016207:Cytokines; D007155:Immunologic Factors; D010936:Plant Extracts; D044004:Xanthones; C021053:mangostin