title: Regional expression of the BCRP/ABCG2 transporter in term human placentas.
abstract: The breast cancer resistance protein (BCRP, ABCG2) is an efflux transporter that removes xenobiotics that cross the placenta back to the maternal circulation, thereby limiting exposure of the fetus to drugs and chemicals. Currently, variability of BCRP expression within the placenta is not known. Ten placentas were collected from healthy women undergoing elective Cesarean sections at term. Villous samples were dissected in defined regions (medial, intermediate, and peripheral) and BCRP mRNA and protein were quantified. There were no regional differences in mRNA expression of housekeeping genes (GAPDH, RPL13a, PRL, 18S). GAPDH had the lowest correlation with BCRP Ct values and was used for BCRP mRNA normalization. No differences in placental BCRP mRNA and protein were observed among the sample sites (<20% variability). Sampling site does not affect the expression of BCRP, supporting the utility of single site sampling protocols to assess the interindividual regulation of this transporter in human placentas.
authors.affiliation: Department of Pediatrics, Rutgers University Robert Wood Johnson Medical School, 1 Robert Wood Johnson Place, New Brunswick, NJ, 08901 USA.
authors.forename: Naureen
authors.initials: N
authors.lastname: Memon
authors.affiliation: Department of Pharmacology and Toxicology, Rutgers University Ernest Mario School of Pharmacy, 170 Frelinghuysen Rd., Piscataway, NJ, 08854 USA.
authors.forename: Kristin M
authors.initials: KM
authors.lastname: Bircsak
authors.affiliation: Department of Pediatrics, Rutgers University Robert Wood Johnson Medical School, 1 Robert Wood Johnson Place, New Brunswick, NJ, 08901 USA.
authors.forename: Faith
authors.initials: F
authors.lastname: Archer
authors.affiliation: Department of Pharmacology and Toxicology, Rutgers University Ernest Mario School of Pharmacy, 170 Frelinghuysen Rd., Piscataway, NJ, 08854 USA.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Gibson
authors.affiliation: Department of Biostatistics, Rutgers University School of Public Health, 683 Hoes Lane W, Piscataway, NJ, 08854 USA.
authors.forename: Pamela
authors.initials: P
authors.lastname: Ohman-Strickland
authors.affiliation: Department of Pediatrics, Rutgers University Robert Wood Johnson Medical School, 1 Robert Wood Johnson Place, New Brunswick, NJ, 08901 USA.
authors.forename: Barry I
authors.initials: BI
authors.lastname: Weinberger
authors.affiliation: Department of Pathology, University of California, San Diego, 200 West Arbor Drive, San Diego, California 92035 USA.
authors.forename: Mana M
authors.initials: MM
authors.lastname: Parast
authors.affiliation: Department of Pediatrics, Rutgers University Robert Wood Johnson Medical School, 1 Robert Wood Johnson Place, New Brunswick, NJ, 08901 USA.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Vetrano
authors.affiliation: Department of Pharmacology and Toxicology, Rutgers University Ernest Mario School of Pharmacy, 170 Frelinghuysen Rd., Piscataway, NJ, 08854 USA.|Environmental and Occupational Health Sciences Institute, 170 Frelinghuysen Rd., Piscataway, NJ, 08854 USA.
authors.forename: Lauren M
authors.initials: LM
authors.lastname: Aleksunes
mesh_terms: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000328:Adult; D005260:Female; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: C116614:ABCG2 protein, human; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; D009363:Neoplasm Proteins; D012333:RNA, Messenger
keywords: ABCG2; BCRP; Housekeeping gene; Placenta; Transporter