title: [Jiedu sangen decoction intervened carcinoma-associated fibroblasts and inhibited migration and invasion of colon cancer: an experimental research].
abstract: OBJECTIVE
  To observe the effect of Jiedu Sangen Decoction (JSD, consisting of Polygonum cuspidatum, Geum Japonicum Thumnb, Radix Actinidiae Chinensis) on the migration capability of colon cancer CT-26 cells were observed, and on expressions of carcinoma-associated fibroblasts (CAFs) such as transforming growth factor-beta1 (TGF-beta1), matrix metalloproteinase 9 (MMP-9), and alpha-smooth muscle actin (alpha-SMA).
  
  
  METHODS
  The BALB/C mice were subcutaneously inoculated with colon cancer CT-26 cells (1.2 x 10(6)mL) and then randomly divided into 3 groups, i.e., the normal control group, the model group, the JSD treated group. The effects of three different serums on the migration ability of colon cancer CT-26 cells were observed using Transwell. The expression quantities of TGF-beta1 and MMP-9 in the supernatant of CAFs were detected using ELISA. The mRNA expression quantities of TGF-beta1 and alpha-SMA in CAFs were detected by real-time fluorescence quantitative PCR.
  
  
  RESULTS
  The number of semi-permeable film cells in the JSD treated group significantly decreased, when compared with the model group, showing statistical significance (P < 0.01). Compared with the model group, the expressions of TGF-beta1 and MMP-9 in the supernatant of CAFs decreased in the JSD treated group at 24 and 48 h, showing statistical difference (P < 0.05, P < 0.01). Compared with the model group, the mRNA expressions of TGF-beta1 and alpha-SMA in the JSD treated group obviously decreased, showing statistical difference (P < 0.01).
  
  
  CONCLUSIONS
  JSD could decrease expressions of TGF-beta1 and MMP-9 in the supernatant of CAFs, lower mRNA expressions of alpha-SMA and TGF-beta1, which might be possible mechanisms for inhibiting the migration and invasion of tumor cells.
authors.affiliation: Department of Tumor, First Affiliated Hospital, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310006, China. smh3288@yahoo.com.cn
authors.forename: Shan-Ming
authors.initials: SM
authors.lastname: Ruan
authors.forename: Min-He
authors.initials: MH
authors.lastname: Shen
authors.forename: Hong
authors.initials: H
authors.lastname: Lin
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Zheng
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Meng-Ting
authors.initials: MT
authors.lastname: Li
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; C508961:Tgfb1 protein, mouse; D053773:Transforming Growth Factor beta1; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse