title: Some characteristics of peroxidase secreted by cotton ovule cultures.
abstract: Cotton (Gossypium hirsutum L.) ovule cultures secreted a soluble peroxidase into the surrounding medium, resulting in a 200-fold increase in this activity during the 30-day growth period. The peroxidase activity was thermostable from 4°C to 60°C and displayed a pH optimum of 5.5 to 6.0. The ovule peroxidase was susceptible to periodate treatment and very resistant to protease digestion. The data suggest that the peroxidase activity is a glycoprotein. Interpretation of peroxidase data may be complicated by the presence of phenol oxidase activity in the same preparations. The presence of phenol oxidases was ruled out by the inaction of a tyrosinase-specific inhibitor, tropolone.
authors.affiliation: US Department of Agriculture, Agriculture Research Service, Southern Regional Research Center, P.O. Box 19687, 70179, New Orleans, LA, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Mellon