title: Interleukin 10 reduces testicular damage in experimental testicular ischemia/reperfusion injury.
abstract: OBJECTIVE
  To evaluate the protective effect of interleukin 10 (IL-10) on biochemical and histopathologic changes in experimental testicular ischemia or reperfusion injury (RI) in rats.
  
  
  METHODS
  Sprague-Dawley rats were randomly divided into 3 groups, each containing 7 rats; sham-control, I-R/untreated group, and I/R treated with IL-10. The ischemia period was 6 hours, and orchiectomy was performed after 1 hour of detorsion. IL-10 was given intraperitoneally in a period of 10 minutes before reperfusion. In all groups, ipsilateral orchiectomies were performed to make histologic examination and biochemical analysis such as malondialdehyde, glutathione peroxidase, and myeloperoxidase (MPO).
  
  
  RESULTS
  IL-10 treatment significantly decreased the I-R-induced elevation in testes malondialdehyde levels. In the I-R/IL-10-treated group, testes glutathione peroxidase levels were increased compared with the I-R/untreated group rats. MPO activities were significantly increased in the testes tissues of the I-R/untreated group. However, in the I-R/IL-10-treated group, MPO levels significantly decreased. Histopathologically, in the I-R/untreated group rats, edema, congestion, hemorrhage among seminiferous tubules, and necrosis of the germinal cells were predominant features in sections. The testicular injury score was lower in the IL-10-treated group rats compared with the I-R/untreated group.
  
  
  CONCLUSIONS
  IL-10 might play a protective role in reducing reperfusion injury.
authors.affiliation: Department of Pediatric Surgery, Abant Izzet Baysal University, Medical School, Bolu, Turkey.
authors.forename: Hulya
authors.initials: H
authors.lastname: Ozturk
authors.affiliation: Department of Pediatric Surgery, Abant Izzet Baysal University, Medical School, Bolu, Turkey. Electronic address: ozturkhayrettin@hotmail.com.
authors.forename: Hayrettin
authors.initials: H
authors.lastname: Ozturk
authors.affiliation: Department of Histology and Embryology, Abant Izzet Baysal University, Medical School, Bolu, Turkey.
authors.forename: Elcin Hakan
authors.initials: EH
authors.lastname: Terzi
authors.affiliation: Department of Biochemistry, Abant Izzet Baysal University, Medical School, Bolu, Turkey.
authors.forename: Guler
authors.initials: G
authors.lastname: Bugdayci
authors.affiliation: Department of Emergency, Abant Izzet Baysal University, Medical School, Bolu, Turkey.
authors.forename: Arif
authors.initials: A
authors.lastname: Duran
mesh_terms: D000818:Animals; D016753:Interleukin-10 / Q000627:therapeutic use*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000209:etiology / Q000517:prevention & control*; D013086:Spermatic Cord Torsion / Q000150:complications; D013737:Testis / Q000098:blood supply*
chemical_list: D016753:Interleukin-10