title: Genetic diversity of Histoplasma and Sporothrix complexes based on sequences of their ITS1-5.8S-ITS2 regions from the BOLD System.
abstract: High sensitivity and specificity of molecular biology techniques have proven usefulness for the detection, identification and typing of different pathogens. The ITS (Internal Transcribed Spacer) regions of the ribosomal DNA are highly conserved non-coding regions, and have been widely used in different studies including the determination of the genetic diversity of human fungal pathogens. This article wants to contribute to the understanding of the intra- and interspecific genetic diversity of isolates of the Histoplasma capsulatum and Sporothrix schenckii species complexes by an analysis of the available sequences of the ITS regions from different sequence databases. ITS1-5.8S-ITS2 sequences of each fungus, either deposited in GenBank, or from our research groups (registered in the Fungi Barcode of Life Database), were analyzed using the maximum likelihood (ML) method. ML analysis of the ITS sequences discriminated isolates from distant geographic origins and particular wild hosts, depending on the fungal species analyzed. This manuscript is part of the series of works presented at the "V International Workshop: Molecular genetic approaches to the study of human pathogenic fungi" (Oaxaca, Mexico, 2012).
authors.affiliation: Colección Nacional de Cultivos Microbianos, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, México DF, Mexico.
authors.forename: Daniel Alfonso
authors.initials: DA
authors.lastname: Estrada-Bárcenas
authors.affiliation: Laboratorio de Inmunología de Hongos, Departamento de Microbiología y Parasitología, Facultad de Medicina, UNAM, México DF, Mexico.
authors.forename: Tania
authors.initials: T
authors.lastname: Vite-Garín
authors.affiliation: Laboratorio de Micología Básica, Departamento de Microbiología y Parasitología, Facultad de Medicina, UNAM, México DF, Mexico.
authors.forename: Hortensia
authors.initials: H
authors.lastname: Navarro-Barranco
authors.affiliation: Laboratorio de Inmunología de Hongos, Departamento de Microbiología y Parasitología, Facultad de Medicina, UNAM, México DF, Mexico.
authors.forename: Raúl
authors.initials: R
authors.lastname: de la Torre-Arciniega
authors.affiliation: Laboratorio de Micología Básica, Departamento de Microbiología y Parasitología, Facultad de Medicina, UNAM, México DF, Mexico.
authors.forename: Amelia
authors.initials: A
authors.lastname: Pérez-Mejía
authors.affiliation: Laboratorio de Inmunología de Hongos, Departamento de Microbiología y Parasitología, Facultad de Medicina, UNAM, México DF, Mexico.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Rodríguez-Arellanes
authors.affiliation: Laboratorio de Inmunología de Hongos, Departamento de Microbiología y Parasitología, Facultad de Medicina, UNAM, México DF, Mexico.
authors.forename: Jose Antonio
authors.initials: JA
authors.lastname: Ramirez
authors.affiliation: Laboratorio de Inmunología de Hongos, Departamento de Microbiología y Parasitología, Facultad de Medicina, UNAM, México DF, Mexico; Unidad de Micología Médica y Experimental, Corporación para Investigaciones Biológicas, Medellín, Colombia.
authors.forename: Jorge
authors.initials: J
authors.lastname: Humberto Sahaza
authors.affiliation: Laboratorio de Inmunología de Hongos, Departamento de Microbiología y Parasitología, Facultad de Medicina, UNAM, México DF, Mexico.
authors.forename: Maria Lucia
authors.initials: ML
authors.lastname: Taylor
authors.affiliation: Laboratorio de Micología Básica, Departamento de Microbiología y Parasitología, Facultad de Medicina, UNAM, México DF, Mexico. Electronic address: toriello@unam.mx.
authors.forename: Conchita
authors.initials: C
authors.lastname: Toriello
mesh_terms: D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D021903:DNA, Ribosomal Spacer; D030541:Databases, Genetic; D014644:Genetic Variation; D006658:Histoplasma / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006660:Histoplasmosis / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D025202:Molecular Diagnostic Techniques; D016533:Mycological Typing Techniques / Q000379:methods*; D013175:Sporothrix / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D013174:Sporotrichosis / Q000175:diagnosis / Q000382:microbiology
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer
keywords: Histoplasma capsulatum; ITS region; Región ITS; Sporothrix schenckii; iBOL