title: Antioxidant action of propolis on mouse lungs exposed to short-term cigarette smoke.
abstract: Propolis is a natural product with antioxidant properties. In this study, we tested the efficacy of propolis against acute lung inflammation (ALI) caused by cigarette smoke (CS). C57BL6 male mice were exposed to CS and treated with propolis (200mg/kg orally, CS+P) or only with propolis (P). A Control group treated with propolis was sham-smoked (Control+P). We collected the lungs for histological and biochemical analyses. We observed an increase in alveolar macrophages and neutrophils in the CS group compared with the Control+P. These counts reduced in the CS+P group compared to the CS group. The treatment with propolis normalized all biochemical parameters in the CS+P group compared with the CS group, including nitrite, myeloperoxidase level, antioxidant enzyme activities (superoxide dismutase, catalase and glutathione peroxidase), reduced glutathione/oxidized glutathione ratio and malondialdehyde. Additionally, TNF-α expression reduced in the CS+P group when compared with the CS group. These data imply a potential antioxidant and anti-inflammatory role for propolis with regard to ALI caused by CS in mice.
authors.affiliation: Programa de Pós-graduação em Biologia Humana e Experimental, Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro, Laboratório de Reparo Tecidual, Av Marechal Rondon 381, Rio de Janeiro, R.J. CEP 20.950-003, Brazil.
authors.forename: Alan Aguiar
authors.initials: AA
authors.lastname: Lopes
authors.forename: Thiago Santos
authors.initials: TS
authors.lastname: Ferreira
authors.forename: Renata Tiscoski
authors.initials: RT
authors.lastname: Nesi
authors.forename: Manuella
authors.initials: M
authors.lastname: Lanzetti
authors.forename: Karla Maria Pereira
authors.initials: KM
authors.lastname: Pires
authors.forename: Ari Miranda
authors.initials: AM
authors.lastname: Silva
authors.forename: Ricardo Moreira
authors.initials: RM
authors.lastname: Borges
authors.forename: Antonio Jorge Ribeiro
authors.initials: AJ
authors.lastname: Silva
authors.forename: Samuel Santos
authors.initials: SS
authors.lastname: Valença
authors.forename: Luís Cristóvão
authors.initials: LC
authors.lastname: Porto
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000378:metabolism / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects; D011429:Propolis / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D014028:Tobacco Smoke Pollution; D011429:Propolis
keywords: Inflammation; Lung; Mice; Oxidative stress; Propolis