title: Aphidicolin as synchronizing agent in root tip meristems of Haplopappus gracilis.
abstract: Aphidicolin, a reversible inhibitor of nuclear DNA replication, was tested as syncrhonizing agent in root tip meristems of Haplopappus gracilis. Embryos (i.e. decoated seeds) or 3-day-old seedlings were used to this purpose. After a 24 h treatment with the drug, a high level of synchrony was observed in both experimental materials for two cell cycles as assessed by determining the accumulation of cells in the S and M phases of the cycle. Highest synchronization was obtained with germinating embryos, possibly owing to a low degree of synchrony already existing in this system.
authors.affiliation: Dipartimento di Biologia, Sezione di Botanica generale, Centro di Studio del C.N.R. per la Biologia Cellulare e Molecolare delle Piante, via Celoria 26, I-20133, Milano, Italia.
authors.forename: M G
authors.initials: MG
authors.lastname: Galli
authors.forename: F
authors.initials: F
authors.lastname: Sala