title: A proteomic study of rice cultivar TNG67 and its high aroma mutant SA0420.
abstract: Fragrance is a very important economic trait for rice cultivars. To identify the aroma genes in rice, we performed a proteomics analysis of aroma-related proteins between Tainung 67 (TNG67) and its high aroma mutant SA0420. Seventeen of the differentially identified proteins were close related with the aroma phenotype of SA0420. Among them, 9 were found in leaves and 8 were found in grains. One protein (L3) was identified as the chloroplastic glyceraldehyde-3-phosphate dehydrogenase B (OsGAPDHB) which was less abundant in SA0420 than TNG67. Sequence analysis demonstrated that this protein in SA0420 carries a P425S mutation in the C-terminal extension domain, which might hinder the formation of holoenzyme, thereby changing the profile of aroma compounds. The protein profile of OsGAPDHB showed only a weak correlation to its transcription profile. This result indicated that the reduction of OsGAPDHB in SA0420 is regulated by post-translational processes and can only be analyzed by proteomics approach. Transgenic lines suppressing OsGAPDHB through RNAi harbored more fragrance than TNG67 but less than SA0420. With betaine-aldehyde dehydrogenase as the only fragrance gene identified in rice to date, OsGAPDHB may serve as the second protein known to contribute to the aroma phenotype.
authors.affiliation: Department of Agronomy, National Chung Hsing University, Taiwan.
authors.forename: Da-Gin
authors.initials: DG
authors.lastname: Lin
authors.forename: Szu-Yi
authors.initials: SY
authors.lastname: Chou
authors.forename: Arthur Z
authors.initials: AZ
authors.lastname: Wang
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Wang
authors.forename: Shu-Ming
authors.initials: SM
authors.lastname: Kuo
authors.forename: Chien-Chen
authors.initials: CC
authors.lastname: Lai
authors.forename: Liang-Jwu
authors.initials: LJ
authors.lastname: Chen
authors.forename: Chang-Sheng
authors.initials: CS
authors.lastname: Wang
mesh_terms: D015152:Blotting, Northern; D060365:Chloroplast Proteins / Q000235:genetics / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000145:classification / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D009812:Odorants; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods*; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D060365:Chloroplast Proteins; D010940:Plant Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases
keywords: 2-Acetyl-1-pyrroline; 2-D Gel Electrophoresis; Aroma rice; Differential display; Glyceraldehyde-3-phosphate dehydrogenase; Oryza sativa L.