title: Implantable fiber-optic interface for parallel multisite long-term optical dynamic brain interrogation in freely moving mice.
abstract: Seeing the big picture of functional responses within large neural networks in a freely functioning brain is crucial for understanding the cellular mechanisms behind the higher nervous activity, including the most complex brain functions, such as cognition and memory. As a breakthrough toward meeting this challenge, implantable fiber-optic interfaces integrating advanced optogenetic technologies and cutting-edge fiber-optic solutions have been demonstrated, enabling a long-term optogenetic manipulation of neural circuits in freely moving mice. Here, we show that a specifically designed implantable fiber-optic interface provides a powerful tool for parallel long-term optical interrogation of distinctly separate, functionally different sites in the brain of freely moving mice. This interface allows the same groups of neurons lying deeply in the brain of a freely behaving mouse to be reproducibly accessed and optically interrogated over many weeks, providing a long-term dynamic detection of genome activity in response to a broad variety of pharmacological and physiological stimuli.
authors.affiliation: 1] International Laser Center, Physics Department, M.V. Lomonosov Moscow State University, Moscow, Russia [2] Kurchatov Institute National Research Center, Moscow, Russia [3] Russian Quantum Center, ul. Novaya 100, Skolkovo, Moscow Region, 1430125 Russia.
authors.forename: L V
authors.initials: LV
authors.lastname: Doronina-Amitonova
authors.forename: I V
authors.initials: IV
authors.lastname: Fedotov
authors.forename: O I
authors.initials: OI
authors.lastname: Ivashkina
authors.forename: M A
authors.initials: MA
authors.lastname: Zots
authors.forename: A B
authors.initials: AB
authors.lastname: Fedotov
authors.forename: K V
authors.initials: KV
authors.lastname: Anokhin
authors.forename: A M
authors.initials: AM
authors.lastname: Zheltikov
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000502:physiology*; D005336:Fiber Optic Technology / Q000295:instrumentation; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D055100:Optical Fibers; D019736:Prostheses and Implants; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D014157:Transcription Factors