title: A simple technique for staining of platyhelminths with the lactophnol cotton blue stain.
abstract: This paper describes a simple technique for staining of flatworms using lactophenol cotton blue (LPCB). The staining was tested on 2 trematode species: Heterophyes heterophyes and Mesostephanus appendiculatus, and one cestode: Diplopylidium acanthotetra, which were collected from the intestine of stray cats in Kuwait. The specimens were mounted in a small amount of the LPCB stain on a clean slide for 2-3 minutes before covering with a cover slip. The technique rapidly and clearly differentiated the internal structures of the helminthes. Its speed and simplicity are advantages over other staining methods. It is easily used in wide-scale surveys where a large number of platyhelminths have to be identified and it is suitable for field studies.
authors.affiliation: Veterinary Laboratories, Public Authority of Agriculture Affairs and Fish Resources, P.O. Box 21422, Safat 13075, Kuwait.
authors.forename: Adawia A M
authors.initials: AA
authors.lastname: Henedi
authors.forename: Osama M E
authors.initials: OM
authors.lastname: El-Azazy
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000469:parasitology; D002415:Cats; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D002589:Cestoda / Q000302:isolation & purification*; D002590:Cestode Infections / Q000469:parasitology / Q000662:veterinary; D004396:Coloring Agents / Q000737:chemistry*; D005990:Glycerol / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods*; D014200:Trematoda / Q000302:isolation & purification*; D014201:Trematode Infections / Q000469:parasitology / Q000662:veterinary
chemical_list: D004396:Coloring Agents; D007211:Indoles; D009942:Organometallic Compounds; D010636:Phenols; C585587:lactophnol cotton blue; D019344:Lactic Acid; D002482:Cellulose; D005990:Glycerol