title: Cytotoxicity evaluation of dentin contacting materials with dentin barrier test device using erbium-doped yttrium, aluminum, and garnet laser-treated dentin.
abstract: OBJECTIVE
  The effect of dentin contacting materials on three-dimensional cultures of pulp-derived cells was evaluated in a dentin barrier test device using erbium-doped yttrium, aluminum, and garnet (Er:YAG) laser-treated dentin.
  
  
  METHODS
  The test materials (iBond(®), G-Bond™, and Vitrebond™) were applied on laser-treated or untreated dentin discs. After 24 h of exposure with perfusion of the test chamber, cell survival was evaluated by enzyme activity and related to a nontoxic control material. The mean values of control tissues were set to represent 100% viability. Data were analyzed using Kruskal-Wallis and Mann-Whitney U test.
  
  
  RESULTS
  Vitrebond was the most toxic material for both laser-treated and untreated dentin. On untreated dentin, G-bond was cytotoxic to the pulp-derived cells (p < 0.05), and iBond was similar to the negative control group (p > 0.05). However, G-Bond and iBond were not cytotoxic when they were applied to Er:YAG laser-treated dentin (p > 0.05).
  
  
  CONCLUSIONS
  Er:YAG laser treatment of dentin may protect the pulp cells from toxic substances of dentin contacting restorative materials; however, this effect is material related. Taking into consideration the limitations of this in vitro study, the Er:YAG laser treatment of dentin before restoration might be an option for decreasing the cytotoxic effects of the dental materials. Further research is required for clinical applications.
authors.affiliation: Department of Restorative Dentistry, Faculty of Dentistry, Selcuk University, Konya, Turkey botsalie@hotmail.com.
authors.forename: H E
authors.initials: HE
authors.lastname: Ülker
authors.affiliation: Department of Restorative Dentistry, Faculty of Dentistry, Selcuk University, Konya, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Ülker
authors.affiliation: Department of Pedodontics, Faculty of Dentistry, Selcuk University, Konya, Turkey.
authors.forename: M S
authors.initials: MS
authors.lastname: Botsalı
authors.affiliation: Department of Restorative Dentistry, Faculty of Dentistry, Abant İzzet Baysal University, Bolu, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Dündar
authors.affiliation: Department of Genetics, Faculty of Medicine, Selcuk University, Konya, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Acar
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000633:toxicity*; D002417:Cattle; D003782:Dental Pulp / Q000166:cytology*; D003804:Dentin / Q000737:chemistry* / Q000648:ultrastructure; D053844:Lasers, Solid-State; D008422:Materials Testing / Q000379:methods*; D008855:Microscopy, Electron, Scanning
chemical_list: D001672:Biocompatible Materials
keywords: Cytotoxicity; Er-YAG laser; dentin barrier; dentin bondings