title: Sex-specific binding and inactivation of agglutination factor in Chlamydomonas eugametos.
abstract: Gametes of opposite mating type (mt (+) and mt (-)) of the green alga Chlamydomonas eugametos agglutinate via their flagella as a prelude to sexual fusion. To quantitate sexual agglutination, an in vitro assay has been developed using (35)S-labeled flagella and the isolated mt (-)agglutination factor. It is shown that not only isolated flagella, but also the mt (-)agglutination factor rapidly bind to the flagella of intact gametes of the opposite mating type. This confirms the role of the mt (-)agglutination factor in determining the sexual agglutinability of mt (-)gametes. As a function of binding, the agglutinative power of the flagella of both mating types is destroyed by a temperature-sensitive process. Likewise, the mt (-)agglutination factor can be completely inactivated.
authors.affiliation: Plantenfysiologisch Laboratorium, Universiteit van Amsterdam, Kruislaan 318, NL-1098 SM, Amsterdam, The Netherlands.
authors.forename: H L
authors.initials: HL
authors.lastname: Fijst
authors.forename: F A
authors.initials: FA
authors.lastname: Ossendorp
authors.forename: P
authors.initials: P
authors.lastname: van Egmond
authors.forename: A M
authors.initials: AM
authors.lastname: Kamps
authors.forename: A
authors.initials: A
authors.lastname: Musgrave
authors.forename: H
authors.initials: H
authors.lastname: van den Ende