title: The effect of sterilization methods on electronspun poly(lactide-co-glycolide) and subsequent adhesion efficiency of mesenchymal stem cells.
abstract: The sterilization of scaffolds is an essential step for tissue engineering in vitro and, mainly, clinical biomaterial use. However, this process can cause changes in the structure and surface of the scaffolds. Therefore, the objective of this study was to investigate the effect of sterilization by ethanol, ultraviolet radiation (UVR) or antimicrobial solution (AMS) on poly(lactide-co-glycolide) (PLGA) scaffolds produced by the electrospinning technique. The properties of nanofibers and the cellular adhesion of mesenchymal stem cells to the scaffolds were analyzed after the treatments. All methods generated sterile scaffolds but showed some kind of damage to the scaffolds. Ethanol and AMS caused changes in the morphology and scaffold dimensions, which were not observed when using the UVR method. However, UVR caused a greater reduction in polymeric molecular weight, which increased proportionally with exposure time of treatment. Nanofibers sterilized with AMS for 1 h and 2 h showed greater cellular adhesion than the other methods, demonstrating their potential as a method for sterilizing PLGA nanofibers.
authors.affiliation: Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, 90610-000, Brazil; Post Graduate Programme in Material Science, Federal University of Rio Grande do Sul, Porto Alegre, 91501-970, Brazil.
authors.forename: Daikelly Iglesias
authors.initials: DI
authors.lastname: Braghirolli
authors.forename: Daniela
authors.initials: D
authors.lastname: Steffens
authors.forename: Kerlin
authors.initials: K
authors.lastname: Quintiliano
authors.forename: Gerson Arisoly Xavier
authors.initials: GA
authors.lastname: Acasigua
authors.forename: Douglas
authors.initials: D
authors.lastname: Gamba
authors.forename: Roland A
authors.initials: RA
authors.lastname: Fleck
authors.forename: Cesar L
authors.initials: CL
authors.lastname: Petzhold
authors.forename: Patricia
authors.initials: P
authors.lastname: Pranke
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002448:Cell Adhesion; D048430:Cell Shape; D002478:Cells, Cultured; D003840:Deoxycholic Acid; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D057139:Nanofibers; D010406:Penicillins / Q000494:pharmacology; D011098:Polyglactin 910 / Q000528:radiation effects; D013242:Sterilization / Q000379:methods*; D013307:Streptomycin / Q000494:pharmacology; D054457:Tissue Scaffolds; D014466:Ultraviolet Rays
chemical_list: D000890:Anti-Infective Agents; D010406:Penicillins; D003840:Deoxycholic Acid; D011098:Polyglactin 910; D000431:Ethanol; D000666:Amphotericin B; D013307:Streptomycin
keywords: PLGA; biomaterials; electrospinning; nanofibers; sterilization