title: Polysaccharide isolated from Agardhiella ramosissima: chemical structure and anti-inflammation activity.
abstract: The sulfated polysaccharide (PLS) fraction of Agardhiella ramosissima was characterized by microanalysis, infrared spectroscopy, NMR and gas-liquid-chromatography-mass-spectrometry. The main constituent of PLS was the ι carrageenan. The monosaccharide composition of the PLS showed galactose, 3,6-anhydrogalactose and 6-O-methylgalactose. The PLS (30 mg kg(-1)) significantly reduced the paw oedema induced by carrageenan, dextran, histamine and serotonin and also was able to significantly inhibit leucocyte migration into the peritoneal cavity and decrease the concentration of myeloperoxidase (MPO) in paw tissue. In the antinociceptive tests, the pre-treatment with PLS reduced the number of writhes, the licking time but did not increase the latency time of response. This study demonstrates for the first time the anti-inflammatory and anti-nociceptive effects of PLS from A. ramosissima. Thus, we concluded that PLS could be a new natural tool in pain and acute inflammatory conditions.
authors.affiliation: LAFFEX - Laboratory of Experimental Physiopharmacology, Biotechnology and Biodiversity Center Research (BIOTEC), Federal University of Piauí-CMRV, Parnaiba, Piauí, Brazil.
authors.forename: Jalles A
authors.initials: JA
authors.lastname: Batista
authors.forename: Eulina G N
authors.initials: EG
authors.lastname: Dias
authors.forename: Tarcisio V
authors.initials: TV
authors.lastname: Brito
authors.forename: Rafael S
authors.initials: RS
authors.lastname: Prudêncio
authors.forename: Renan O
authors.initials: RO
authors.lastname: Silva
authors.forename: Ronaldo A
authors.initials: RA
authors.lastname: Ribeiro
authors.forename: Marcellus Henrique L P
authors.initials: MH
authors.lastname: Souza
authors.forename: Regina C M
authors.initials: RC
authors.lastname: de Paula
authors.forename: Judith P A
authors.initials: JP
authors.lastname: Feitosa
authors.forename: Luciano S
authors.initials: LS
authors.lastname: Chaves
authors.forename: Márcia R S
authors.initials: MR
authors.lastname: Melo
authors.forename: Ana L P
authors.initials: AL
authors.lastname: Freitas
authors.forename: Jand-Venes R
authors.initials: JV
authors.lastname: Medeiros
authors.forename: André L R
authors.initials: AL
authors.lastname: Barbosa
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002351:Carrageenan; D002465:Cell Movement / Q000187:drug effects; D003911:Dextrans; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D005690:Galactose / Q000031:analogs & derivatives / Q000737:chemistry; D006614:Hindlimb; D006632:Histamine; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D008297:Male; D008756:Methylgalactosides / Q000737:chemistry; D051379:Mice; D059225:Nociception / Q000187:drug effects / Q000502:physiology; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D009195:Peroxidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000461:Rhodophyta / Q000737:chemistry*; D012701:Serotonin; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000893:Anti-Inflammatory Agents; D003911:Dextrans; D008756:Methylgalactosides; D010936:Plant Extracts; D011134:Polysaccharides; D012701:Serotonin; C117625:3,6-anhydrogalactose; D006632:Histamine; D002351:Carrageenan; D009195:Peroxidase; D005690:Galactose
keywords: Agardhiella ramosissima; Anti-inflammatory effect; Antinociceptive action; Chemical structure; Polysaccharide