title: Plantlet regeneration from glume calli of maize (Zea mays L.).
abstract: Totipotent callus cultures were established from anther-free glumes of 'Sweet corn', 'Seed corn,' 'DHM 103' and 'DHM 101' on MS medium supplemented with 1-2 mg/l 2,4-D. The callusing response of the glumes was tested on six different media. Glumes at the uninucleate stage of pollen development callused with a high frequency compared to other stages. Organogenesis was observed in 40% of the cultures on media devoid of hormones. A total of 76 plantlets were regenerated on medium with 0.5-1.0 mg/l of both IAA and kinetin. Cytological observations in root tips indicated a diploid chromosome number (2n=20).
authors.affiliation: Department of Genetics, Osmania University, 500 007, Hyderabad, India.
authors.forename: P
authors.initials: P
authors.lastname: Suprasanna
authors.forename: K V
authors.initials: KV
authors.lastname: Rao
authors.forename: G M
authors.initials: GM
authors.lastname: Reddy