title: Radiation-modifying abilities of Nigella sativa and thymoquinone on radiation-induced nitrosative stress in the brain tissue.
abstract: To investigate Nigella sativa oil (NSO) and Thymoquinone (TQ) for their antioxidant effects on the brain tissue of rats exposed to ionizing radiation. Fifty-four male albino Wistar rats, divided into six groups, were designed as group I (normal control group) did not receive NSO, TQ or irradiation; group II (control group of TQ) received dimethyl sulfoxide and sham irradiation; group III (control group of NSO) received saline and sham irradiation; group IV (irradiation plus NSO group) received both 5 Gray of gamma irradiation to total cranium and NSO; group V (irradiation plus TQ group) received both irradiation and TQ; group VI (irradiation alone group) received irradiation plus saline. Alterations in nitric oxide (NO·) and peroxynitrite (ONOO(-)) levels, and nitric oxide synthase (NOS) enzyme activity were measured by biochemical methods in homogenized brain tissue of rats. Levels of NO· and ONOO(-), and enzyme activity of NOS in brain tissue of the rats treated with NSO or TQ were found to be lower than in received IR alone (p<0.002) Nigella sativa oil (NSO) and its active component, TQ, clearly protect brain tissue from radiation-induced nitrosative stress.
authors.affiliation: Department of Radiation Oncology, Gaziantep University, Medical School, Gaziantep, Turkey.
authors.forename: Adem
authors.initials: A
authors.lastname: Ahlatci
authors.affiliation: Department of Radiation Oncology, Gaziantep University, Medical School, Gaziantep, Turkey. Electronic address: a_kuzhan@hotmail.com.
authors.forename: Abdurahman
authors.initials: A
authors.lastname: Kuzhan
authors.affiliation: Department of Biochemistry and Clinical Biochemistry, Gaziantep University, Medical School, Gaziantep, Turkey.
authors.forename: Seyithan
authors.initials: S
authors.lastname: Taysi
authors.affiliation: Department of Biophysics, Gaziantep University, Medical School, Gaziantep, Turkey.
authors.forename: Omer Can
authors.initials: OC
authors.lastname: Demirtas
authors.affiliation: Department of Radiation Oncology, Gaziantep University, Medical School, Gaziantep, Turkey.
authors.forename: Hilal Eryigit
authors.initials: HE
authors.lastname: Alkis
authors.affiliation: Department of Biochemistry and Clinical Biochemistry, Gaziantep University, Medical School, Gaziantep, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Tarakcioglu
authors.affiliation: Department of Radiation Oncology, Gaziantep University, Medical School, Gaziantep, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Demirci
authors.affiliation: Department of Radiation Oncology, Gaziantep University, Medical School, Gaziantep, Turkey.
authors.forename: Derya
authors.initials: D
authors.lastname: Caglayan
authors.affiliation: Department of Biochemistry, Dr. Ersin Arslan State Hospital, Gaziantep, Turkey.
authors.forename: Edibe
authors.initials: E
authors.lastname: Saricicek
authors.affiliation: Department of Neurosurgery, Sehitkamil State Hospital, Gaziantep, Turkey.
authors.forename: Kadir
authors.initials: K
authors.lastname: Cinar
mesh_terms: D000818:Animals; D016227:Benzoquinones / Q000494:pharmacology / Q000627:therapeutic use*; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D001930:Brain Injuries / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D004353:Drug Evaluation, Preclinical; D008297:Male; D008517:Phytotherapy; D010938:Plant Oils / Q000494:pharmacology / Q000627:therapeutic use*; D011833:Radiation Injuries, Experimental / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D011897:Random Allocation; D017208:Rats, Wistar; D026361:Reactive Nitrogen Species / Q000009:adverse effects*; D029974:Carum
chemical_list: D016227:Benzoquinones; D015415:Biomarkers; D010938:Plant Oils; D026361:Reactive Nitrogen Species; C104525:caraway oil; C003466:thymoquinone
keywords: Brain; Ionizing radiation; Nigella sativa; Nitric oxide; Nitrosative stress; Thymoquinone