title: Simultaneous quantification and peroxynitrite-scavenging activities of flavonoids in Polygonum aviculare L. herb.
abstract: The plant Polygonum aviculare L. (Polygonaceae) is an annual herbaceous plant which is known to be beneficial for treating gastroduodenal ulcer, hypertension, diarrhea, hemorrhage, and hemorrhoids. Ten phenolic compounds, including nine flavonoids (myricetin, quercetin, kaempferol, myricitrin, desmanthin-1, isoquercitrin, quercitrin, avicularin, juglanin), and gallic acid were used for simultaneous HPLC quantification and peroxynitrite-scavenging assay. Simultaneous quantification of these substances were performed on five extracts (EtOH-, MeOH-, 70% MeOH-, 30% MeOH-, and H2O extracts) as well as on the three fractions (Et2O-, EtOAc-, and BuOH fractions), under the condition of a Capcell Pak C18 column (5μm, 250mm×4.6mm i.d.) and a gradient elution of 0.05% trifluoroacetic acid (TFA) and MeOHCH3CN (60:40). Of the three fractions, the EtOAc fraction displayed the highest content of flavonoids (sum, 208.9mg/g) with the strongest peroxynitrite-scavenging activity (IC50, 2.68μg/mL). The activities of the eight compounds (myricitrin, isoquercitrin, avicularin, quercitrin, myricetin, desmanthin-1, quercetin, and kaempferol) were comparable to that of the positive control (l-penicillamine; IC50: 1.03μg/mL). These results suggest that folkloric medicinal uses of P. aviculare are mainly attributed to flavonoids, such as particularly highly contained myricetin, myricitrin, and desmanthin-1.
authors.affiliation: Department of Applied Plant Sciences, Graduate School, Sangji University, Wonju 220-702, Republic of Korea; Department of Agro-industrial Technology, Faculty of Agriculture, Lambung Mangkurat University, Banjarbaru 70712, Indonesia.
authors.forename: Agung
authors.initials: A
authors.lastname: Nugroho
authors.affiliation: Department of Food Science and Nutrition, Pukyong National University, Busan 607-737, Republic of Korea.
authors.forename: Eon Ji
authors.initials: EJ
authors.lastname: Kim
authors.affiliation: Department of Food Science and Nutrition, Pukyong National University, Busan 607-737, Republic of Korea.
authors.forename: Jae Sue
authors.initials: JS
authors.lastname: Choi
authors.affiliation: Department of Pharmaceutical Engineering, Sangji University, Wonju 220-702, Republic of Korea. Electronic address: hjpark@sangji.ac.kr.
authors.forename: Hee-Juhn
authors.initials: HJ
authors.lastname: Park
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005419:Flavonoids / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry*; D030421:Peroxynitrous Acid / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D028502:Polygonum / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts; D030421:Peroxynitrous Acid
keywords: Desmanthin-1; Flavonoids; HPLC; Peroxynitrite; Polygonaceae; Polygonum aviculare