title: Efficient hybridization between Lycopersicon esculentum and L. peruvianum via embryo callus.
abstract: Embryo callus was produced in up to 12% of the undeveloped seeds of the interspecific cross L. esculentum cv. VFNT cherry x L. peruvianum LA1283-4, a cross which does not produce viable seeds. Plants were produced from 90% of those callus clones by regeneration and rooting. Evidence that these plants were hybrid includes morphology, isoenzyme patterns and fertility relations. Both diploid and tetraploid plants were produced, 40% of the regenerated callus clones producing at least one diploid plant. Thus, up to 4% of the undeveloped seeds plated for callus production eventually yielded diploid hybrid plants. In contrast, among 401 undeveloped seeds dissected, no embryos suitable for embryo culture were found. A backcross of the embryo callus hybrids to the L. esculentum parent has succeeded - producing, as expected, only undeveloped seeds; these undeveloped seeds have produced callus.
authors.affiliation: Department of Bacteriology, University of California, Davis, California, USA.
authors.forename: B R
authors.initials: BR
authors.lastname: Thomas
authors.forename: D
authors.initials: D
authors.lastname: Pratt