title: Adhesion of Candida biofilm cells to human epithelial cells and polystyrene after treatment with silver nanoparticles.
abstract: This study investigated the adhesion to human epithelial cells and polystyrene surface of viable yeasts recovered from Candida biofilms treated with silver nanoparticles (SN). Biofilm resuspended Candida cells were added to HeLa cells or to empty wells of microtiter plates and the adhesion was verified using crystal violet staining. The adhesion of Candida cells was significantly reduced, mainly when biofilms were pretreated with 54 μg/mL SN. These new findings allow to conclude that SN may induce changes in viable yeasts, which can decrease the dissemination of Candida infections, mainly in susceptible patients.
authors.affiliation: Department of Dental Materials and Prosthodontics, Araçatuba Dental School, Univ Estadual Paulista (UNESP), Araçatuba 16015-050, São Paulo, Brazil; Institute for Biotechnology and Bioengineering, Department of Biological Engineering, University of Minho, Braga 4710-057, Portugal.
authors.forename: Douglas Roberto
authors.initials: DR
authors.lastname: Monteiro
authors.affiliation: Institute for Biotechnology and Bioengineering, Department of Biological Engineering, University of Minho, Braga 4710-057, Portugal; Faculdade INGÁ, Maringá 87070-000, Paraná, Brazil.
authors.forename: Melyssa
authors.initials: M
authors.lastname: Negri
authors.affiliation: Institute for Biotechnology and Bioengineering, Department of Biological Engineering, University of Minho, Braga 4710-057, Portugal.
authors.forename: Sónia
authors.initials: S
authors.lastname: Silva
authors.affiliation: LIEC-Department of Chemistry, Federal University of São Carlos (UFSCar), São Carlos 13565-905, São Paulo, Brazil.
authors.forename: Luiz Fernando
authors.initials: LF
authors.lastname: Gorup
authors.affiliation: LIEC-Department of Chemistry, Federal University of São Carlos (UFSCar), São Carlos 13565-905, São Paulo, Brazil.
authors.forename: Emerson Rodrigues
authors.initials: ER
authors.lastname: de Camargo
authors.affiliation: Institute for Biotechnology and Bioengineering, Department of Biological Engineering, University of Minho, Braga 4710-057, Portugal.
authors.forename: Rosário
authors.initials: R
authors.lastname: Oliveira
authors.affiliation: Department of Dental Materials and Prosthodontics, Araçatuba Dental School, Univ Estadual Paulista (UNESP), Araçatuba 16015-050, São Paulo, Brazil.
authors.forename: Debora Barros
authors.initials: DB
authors.lastname: Barbosa
authors.affiliation: Institute for Biotechnology and Bioengineering, Department of Biological Engineering, University of Minho, Braga 4710-057, Portugal. Electronic address: mcrh@deb.uminho.pt.
authors.forename: Mariana
authors.initials: M
authors.lastname: Henriques
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D002175:Candida / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D050296:Microbial Viability / Q000187:drug effects; D011137:Polystyrenes / Q000494:pharmacology*; D012834:Silver / Q000494:pharmacology*
chemical_list: D011137:Polystyrenes; D012834:Silver
keywords: Candida; Epithelial cells; Polystyrene; Silver nanoparticles; Yeast adhesion