title: Extraction of polysaccharides from Phellinus nigricans mycelia and their antioxidant activities in vitro.
abstract: In this study, response surface methodology was employed to optimize the extraction of polysaccharides from Phellinus nigricans mycelia. A central composite design was adopted to determine optimum parameters (extraction time, extraction temperature, extraction frequency, and ratio of water to raw material) that could yield a maximum polysaccharide. Results revealed the following optimum extraction conditions: extraction time, 2.8h; ratio of water to raw material, 28; extraction frequency, 5; and extraction temperature, 95 °C. Under optimized conditions, the experimental yield of P. nigricans mycelia polysaccharides was 15.33 ± 0.21%, which is consistent with the predicted yield. The antioxidant activity assay in vitro showed that the polysaccharides exhibited a high scavenging activity against superoxide anion, hydroxyl, and 1,1-diphenyl-2-picrylhydrazyl radicals. These polysaccharides also exhibited a strong reducing power. Thus, these polysaccharides can be used as natural antioxidants in functional foods or medicine.
authors.affiliation: School of Environmental and Biological Engineering, Liaoning Shihua University, Fushun 113001, China. Electronic address: wangzy125@lshu.edu.cn.
authors.forename: Zhanyong
authors.initials: Z
authors.lastname: Wang
authors.forename: Chenyu
authors.initials: C
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Quan
mesh_terms: D001487:Basidiomycota / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors / Q000737:chemistry; D005163:Factor Analysis, Statistical; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors / Q000737:chemistry; D025282:Mycelium / Q000737:chemistry*; D010851:Picrates / Q000037:antagonists & inhibitors / Q000737:chemistry; D013481:Superoxides / Q000037:antagonists & inhibitors / Q000737:chemistry; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D062610:Fungal Polysaccharides; D010851:Picrates; D014867:Water; D013481:Superoxides; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant; Mycelia; Phellinus nigricans; Polysaccharides; Response surface methodology