title: Preparation and characterisation of type I and V collagens from the skin of Amur sturgeon (Acipenser schrenckii).
abstract: The collagen in Amur sturgeon was extracted by pepsin digestion and separated into two fractions, P₂.₄ (92.40%) and P₄.₀ (2.16%), by sodium chloride precipitation. SDS-PAGE and amino acid profile suggested that the P₂.₄ and P₄.₀ might be classified as type I collagen (PSC-I) and type V collagen (PSC-V), respectively. These collagens appeared to be dense sheet-like film linked by random-coiled filaments under SEM. The denaturation and melting temperatures of PSC-V (35.92 and 122.86 °C) were significantly higher than PSC-I (32.52 and 116.01 °C) assessed by CD and DSC, which could be attributed to its higher imino acid content (23.43%) and degree of hydroxylation (52.18%). FTIR confirmed their triple helical structure, and indicated more intermolecular crosslinks in PSC-I and more hydrogen bond in PSC-V. These results provide some basis for their large-scale production and further application as alternatives to mammalian collagen.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Qiufang
authors.initials: Q
authors.lastname: Liang
authors.forename: Zhenbin
authors.initials: Z
authors.lastname: Wang
authors.forename: Junmin
authors.initials: J
authors.lastname: Xu
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Haile
authors.initials: H
authors.lastname: Ma
mesh_terms: D000818:Animals; D003094:Collagen / Q000737:chemistry* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D029941:Fish Proteins / Q000737:chemistry* / Q000302:isolation & purification; D005399:Fishes; D006860:Hydrogen Bonding; D017433:Protein Structure, Secondary; D012867:Skin / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D029941:Fish Proteins; D003094:Collagen
keywords: Amur sturgeon (Acipenser schrenckii); Characterisation; Collagen; Preparation