title: Intact expression status of RASSF1A in acute myeloid leukemia.
abstract: As a typical tumor suppressor gene, transcriptional silencing of ras-association domain family 1, isoform A (RASSF1A) is caused by biallelic methylation or the condition that one allele is methylated and then the other allele lost by allelic loss, as second hit. RASSF1A is inactivated epigenetically and thus down-regulated in many solid tumors. In summary, for the first time, we analyzed the expression status of RASSF1A in a cohort of 56 de novo acute myeloid leukemia (AML) patients using quantitative real-time polymerase chain reaction. Results of our study indicate that patients with AML exhibited no differences in the RASSF1A gene expression comparing to normal controls. In conclusion, expression status of RASSF1A remained intact in our target samples, indicating that RASSF1A expression variation does not participate in the pathogenesis and the progression of AML.
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran.
authors.forename: Davood
authors.initials: D
authors.lastname: Zare-Abdollahi
authors.forename: Shamsi
authors.initials: S
authors.lastname: Safari
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Movafagh
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Ghadiani
authors.forename: Sahand
authors.initials: S
authors.lastname: Riazi-Isfahani
authors.forename: Mir Davood
authors.initials: MD
authors.lastname: Omrani
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D018450:Disease Progression; D044127:Epigenesis, Genetic; D005260:Female; D015973:Gene Expression Regulation, Leukemic; D020868:Gene Silencing; D016147:Genes, Tumor Suppressor; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D020033:Protein Isoforms; C411762:RASSF1 protein, human; D025521:Tumor Suppressor Proteins