title: Free radical scavenging, antiproliferative activities and profiling of variations in the level of phytochemicals in different parts of broccoli (Brassica oleracea italica).
abstract: In the present study, the seeds of Broccoli (cultivar Palam samridhi) at different stages of development were being analysed for their antioxidant and antiproliferative properties. Among the antioxidant assays performed, a remarkable inhibition of superoxide radicals i.e. 94.25% observed with extracts of five days old sprout (PS5) at 2 mg/ml concentration. Although, all the extracts showed high cytotoxicity but the floret extract (PSF) found to be most effective with IC₅₀ value of 25.94 μg/ml while leaves extract (PSL) was least effective. The cell cycle analysis showed increased G₀/G₁ phase population as compare to positive control camptothecin. Profiling of various phytochemicals executed by using gas chromatography-mass spectroscopy in order to correlate the bioactivities of the extracts. A wide variation observed in the profile of GLS hydrolytic products of different extracts obtained from the seeds, sprouts (three, five and seven days), leaves and florets.
authors.affiliation: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143 005, Punjab, India. Electronic address: ashun.chaudhary@gmail.com.
authors.forename: Ashun
authors.initials: A
authors.lastname: Chaudhary
authors.forename: Upendra
authors.initials: U
authors.lastname: Sharma
authors.forename: Adarsh Pal
authors.initials: AP
authors.lastname: Vig
authors.forename: Bikram
authors.initials: B
authors.lastname: Singh
authors.forename: Saroj
authors.initials: S
authors.lastname: Arora
mesh_terms: D001937:Brassica / Q000737:chemistry*; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D006131:Growth Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D012639:Seeds / Q000737:chemistry
chemical_list: D016166:Free Radical Scavengers; D006131:Growth Inhibitors; D010936:Plant Extracts
keywords: 2′,7′-dichlorofluorescin diacetate; Antioxidant assays; Broccoli; Cell cycle analysis; DCFH-DA; GLs; Glucosinolates; ITCs; Isothiocyanates; P I; PS3; PS5; PS7; PSF; PSL; PSS; ROS; glucosinolates; isothiocyanates; palam samridhi five days; palam samridhi floret; palam samridhi leaves; palam samridhi seeds; palam samridhi seven days; palam samridhi three days; propidium iodide