title: Transcriptome analysis of leaf tissue of Raphanus sativus by RNA sequencing.
abstract: Raphanus sativus is not only a popular edible vegetable but also an important source of medicinal compounds. However, the paucity of knowledge about the transcriptome of R. sativus greatly impedes better understanding of the functional genomics and medicinal potential of R. sativus. In this study, the transcriptome sequencing of leaf tissues in R. sativus was performed for the first time. Approximately 22 million clean reads were generated and used for transcriptome assembly. The generated unigenes were subsequently annotated against gene ontology (GO) database. KEGG analysis further revealed two important pathways in the bolting stage of R.sativus including spliceosome assembly and alkaloid synthesis. In addition, a total of 6,295 simple sequence repeats (SSRs) with various motifs were identified in the unigene library of R. sativus. Finally, four unigenes of R. sativus were selected for alignment with their homologs from other plants, and phylogenetic trees for each of the genes were constructed. Taken together, this study will provide a platform to facilitate gene discovery and advance functional genomic research of R. sativus.
authors.affiliation: School of Life Science and Techonology, Huazhong University of Science and Technology, Wuhan, Hubei, China ; Institute of Biology and Medicine, Wuhan University of Science and Technology, Wuhan, Hubei, China.
authors.forename: Libin
authors.initials: L
authors.lastname: Zhang
authors.forename: Haibo
authors.initials: H
authors.lastname: Jia
authors.forename: Yongtai
authors.initials: Y
authors.lastname: Yin
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.forename: Heng
authors.initials: H
authors.lastname: Xia
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Wang
authors.forename: Chunhua
authors.initials: C
authors.lastname: Fu
authors.forename: Maoteng
authors.initials: M
authors.lastname: Li
authors.forename: Jiangsheng
authors.initials: J
authors.lastname: Wu
mesh_terms: D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D018895:Microsatellite Repeats; D058977:Molecular Sequence Annotation; D010802:Phylogeny; D018515:Plant Leaves; D031224:Raphanus / Q000145:classification / Q000235:genetics*; D017423:Sequence Analysis, RNA; D013045:Species Specificity; D059467:Transcriptome