title: Proteomic characteristics of human sperm cryopreservation.
abstract: Human sperm cryopreservation in assisted reproductive technology is the only proven method that enables infertile men to father their own children. However, freezing and thawing reduces spermatozoon motility, viability, and fertilizing ability. An association between dysfunctional spermatozoa due to cryoinjury and protein changes has not been established. We investigated through proteomic analysis the differential protein characteristics between freeze-thawed and fresh sperm samples obtained from nine normozoospermic donors. Twenty-seven proteins differed in abundance between the two groups, and results were verified for four proteins via Western blot and immunofluorescent staining. These proteins are putatively involved in sperm motility, viability, acrosomal integrity, ATP and isocitrate content, mitochondrial membrane potential, capacitation, acrosome reaction, and intracellular calcium concentration. These marked differences suggest that dysfunctional spermatozoon after cryopreservation may be due to protein degradation and protein phosphorylation.
authors.affiliation: State Key Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, P. R. China.
authors.forename: Shangqian
authors.initials: S
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.forename: Min
authors.initials: M
authors.lastname: Tang
authors.forename: Jianzheng
authors.initials: J
authors.lastname: Fang
authors.forename: Hongyong
authors.initials: H
authors.lastname: Sun
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Sun
authors.forename: Meijuan
authors.initials: M
authors.lastname: Gu
authors.forename: Zhili
authors.initials: Z
authors.lastname: Liu
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Zhang
authors.forename: Faxi
authors.initials: F
authors.lastname: Lin
authors.forename: Ting
authors.initials: T
authors.lastname: Wu
authors.forename: Ninghong
authors.initials: N
authors.lastname: Song
authors.forename: Zengjun
authors.initials: Z
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Changjun
authors.initials: C
authors.lastname: Yin
mesh_terms: D020101:Acrosome Reaction; D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D015925:Cryopreservation / Q000379:methods; D006801:Humans; D008297:Male; D053078:Membrane Potential, Mitochondrial; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics; D012662:Semen Preservation / Q000379:methods; D013081:Sperm Motility; D013094:Spermatozoa / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D055815:Young Adult
chemical_list: D020543:Proteome; D000255:Adenosine Triphosphate
keywords: Biomedicine; Mechanism; Sperm cryopreservation