title: Inhibition of Binding of β2-Glycoprotein 1 to Phosphatidylserine by Polymyxin B, a Lupus-Like Anticoagulant.
abstract: Polymyxin B is a cationic peptide that inhibits phospholipid-dependent coagulation tests including activated partial thromboplastin time and to a lesser degree prothrombin time. Thrombin clotting time is insensitive to polymyxin B. β2-glycoprotein 1 (β2GP1) is a cofactor of antiphospholipid antibodies. Antiphospholipid autoantibodies also poses lupus anticoagulant activity through interactions with β2GP1. Using affinity chromatography, polymyxin B can effectively decrease the binding of β2GP1 to immobilize phosphatidylserine. Since then, anticoagulant effect of polymyxin B is most likely due to the binding to negatively charged phospholipids, preventing formation of coagulation complexes.
authors.affiliation: Department of Pathology and Laboratory Medicine, Indiana University Health, Indianapolis, IN, USA buchman@clarian.org.
authors.forename: Boguslaw
authors.initials: B
authors.lastname: Uchman
authors.affiliation: Deceased.
authors.forename: Douglas A
authors.initials: DA
authors.lastname: Triplett
mesh_terms: D006801:Humans; D016682:Lupus Coagulation Inhibitor / Q000737:chemistry* / Q000378:metabolism; D010718:Phosphatidylserines / Q000737:chemistry* / Q000378:metabolism; D011112:Polymyxin B / Q000737:chemistry*; D011485:Protein Binding; D053482:beta 2-Glycoprotein I / Q000737:chemistry* / Q000378:metabolism
chemical_list: D016682:Lupus Coagulation Inhibitor; D010718:Phosphatidylserines; D053482:beta 2-Glycoprotein I; D011112:Polymyxin B
keywords: lupus-like anticoagulants; polymyxin B; β2-glycoprotein 1.