title: Distribution of extracellular matrix macromolecules in the vestibular nuclei and cerebellum of the frog, Rana esculenta.
abstract: The axons of transected and re-apposed vestibulocochlear nerve of the frog, in contrast to mammalian species, regenerate and establish functional contacts within their original termination areas of the vestibular nuclear complex and the cerebellum. The lack of regenerative capability of the mammalian central nervous system (CNS) is partially attributed to various extracellular matrix (ECM) molecules, such as chondroitin sulfate proteoglycans (CSPG) and tenascin-R (TN-R), which exert inhibition on axon regeneration. In contrast to these molecules, hyaluronan (HA) was reported to be permissive for CNS regeneration. Using histochemical and immunohistochemical methods, we investigated the distribution pattern of these molecules in the medial (MVN), lateral (LVN), superior and descending vestibular nuclei and the cerebellum of the frog and detected regional differences in the organization of the ECM. In the vestibular nuclear complex, pericellular condensation of the ECM, the perineuronal nets (PNNs) were recognizable in the LVN and MVN and were positive only for HA. The neuropil of the vestibular nuclei showed either a diffuse appearance with varying intensity of reactions, or dots and ring-like structures, which may represent the perinodal ECM of the vestibular fibers. In the cerebellum, indistinct PNNs that were only labeled for HA were present in the granular layer. Our findings suggest that the HA-rich, but CSPG and TN-R-free PNNs may be associated with the high degree of plasticity and regenerative potential of the amphibian vestibular system.
authors.affiliation: Department of Anatomy, University of Debrecen, Medical and Health Science Center, Nagyerdei krt. 98, Debrecen 4032, Hungary.
authors.forename: B
authors.initials: B
authors.lastname: Gaál
authors.affiliation: Department of Anatomy, University of Debrecen, Medical and Health Science Center, Nagyerdei krt. 98, Debrecen 4032, Hungary.
authors.forename: É
authors.initials: É
authors.lastname: Rácz
authors.affiliation: Department of Anatomy, University of Debrecen, Medical and Health Science Center, Nagyerdei krt. 98, Debrecen 4032, Hungary.
authors.forename: T
authors.initials: T
authors.lastname: Juhász
authors.affiliation: Department of Anatomy, University of Debrecen, Medical and Health Science Center, Nagyerdei krt. 98, Debrecen 4032, Hungary.
authors.forename: K
authors.initials: K
authors.lastname: Holló
authors.affiliation: Department of Anatomy, University of Debrecen, Medical and Health Science Center, Nagyerdei krt. 98, Debrecen 4032, Hungary; MTA-DE Neuroscience Research Group, University of Debrecen, Debrecen 4032, Hungary. Electronic address: matesz@anat.med.unideb.hu.
authors.forename: C
authors.initials: C
authors.lastname: Matesz
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002531:Cerebellum / Q000378:metabolism*; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D011509:Proteoglycans / Q000378:metabolism; D011893:Rana esculenta; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity; D019063:Tenascin / Q000378:metabolism; D014726:Vestibular Nuclei / Q000378:metabolism*; D000099249:Proteoglycan Link Protein
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D019063:Tenascin; D000099249:Proteoglycan Link Protein; C074638:tenascin R
keywords: CNS; CSPG; DVN; ECM; HA; LVN; MVN; NHS; PNN; SVN; TN-R; WFA; Wisteria floribunda agglutinin; bHABP; bWFA; biotinylated Wisteria floribunda agglutinin; biotinylated hyaluronan-binding protein; brainstem; central nervous system; chondroitin sulfate proteoglycan; descending vestibular nucleus; extracellular matrix; hyaluronan; lateral vestibular nucleus; medial vestibular nucleus; neural plasticity; normal horse serum; perineuronal net; superior vestibular nucleus; tenascin-R