title: The rearrangement of filamentous actin in mossy fiber synapses in pentylenetetrazol-kindled C57BL/6 mice.
abstract: Chemical kindling, as an experimental model of epileptogenesis, is induced by repetitive administration of subconvulsive amount of excitatory drugs. Kindled mice do not typically display spontaneous recurrent seizures, but are instead characterized by enhanced seizure susceptibility to convulsive stimulations. In order to provide insights into the aberrant synaptic plasticity during kindling, this study investigated the effect of pentylenetetrazol (PTZ) kindling on filamentous actin (F-actin) in mossy fiber synapses in C57BL/6 mice. Phalloidin labeling of F-actin showed that F-actin puncta were increased in number in the stratum lucidum of CA3 region in the hippocampus after kindling. The rearrangement of F-actin seemed to occur presynaptically, since synapsin I, a specific marker for mossy fiber terminals, was also up-regulated. Such subtle structural modifications occurring in the synapses are thought to contribute to the long-lasting increased sensitivity in the PTZ-kindled C57BL/6 mice.
authors.affiliation: Department of Histology and Embryology, College of Basic Medical Sciences, Norman Bethune Health Science Center of Jilin University, Changchun, Jilin Province 130021, PR China; Department of Neurology, First Affiliated Hospital of Jilin University, Changchun, Jilin Province 130021, PR China; Department of Pediatric Neurology, First Hospital of Jilin University, Changchun, Jilin Province 130021, PR China.
authors.forename: Yan-Feng
authors.initials: YF
authors.lastname: Zhang
authors.affiliation: Department of Histology and Embryology, College of Basic Medical Sciences, Norman Bethune Health Science Center of Jilin University, Changchun, Jilin Province 130021, PR China.
authors.forename: Shu-Lei
authors.initials: SL
authors.lastname: Li
authors.affiliation: Department of Histology and Embryology, College of Basic Medical Sciences, Norman Bethune Health Science Center of Jilin University, Changchun, Jilin Province 130021, PR China.
authors.forename: Tian-Qing
authors.initials: TQ
authors.lastname: Xiong
authors.affiliation: Department of Pediatric Neurology, First Hospital of Jilin University, Changchun, Jilin Province 130021, PR China.
authors.forename: Li-Bin
authors.initials: LB
authors.lastname: Yang
authors.affiliation: Department of Neurology, Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province 150001, PR China.
authors.forename: Yong-Nan
authors.initials: YN
authors.lastname: Li
authors.affiliation: Laboratory Teaching Center of Basic Medicine, College of Basic Medical Sciences, Norman Bethune Health Science Center of Jilin University, Changchun, Jilin Province 130021, PR China.
authors.forename: Bai-Hong
authors.initials: BH
authors.lastname: Tan
authors.affiliation: Department of Neurology, First Affiliated Hospital of Jilin University, Changchun, Jilin Province 130021, PR China. Electronic address: Liuqun7654321@126.com.
authors.forename: Qun
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Histology and Embryology, College of Basic Medical Sciences, Norman Bethune Health Science Center of Jilin University, Changchun, Jilin Province 130021, PR China. Electronic address: liyanchao@jlu.edu.cn.
authors.forename: Yan-Chao
authors.initials: YC
authors.lastname: Li
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000473:pathology*; D000818:Animals; D056654:CA3 Region, Hippocampal / Q000187:drug effects / Q000473:pathology; D007696:Kindling, Neurologic / Q000187:drug effects / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019599:Mossy Fibers, Hippocampal / Q000187:drug effects / Q000473:pathology*; D010433:Pentylenetetrazole / Q000633:toxicity*; D011897:Random Allocation; D012640:Seizures / Q000139:chemically induced / Q000473:pathology*; D013569:Synapses / Q000187:drug effects / Q000473:pathology*
chemical_list: D010433:Pentylenetetrazole
keywords: Chemical kindling; Filamentous actin; Hippocampus; Pentylenetetrazol; Synapsin