title: Unilateral partial limbal stem cell deficiency: contralateral versus ipsilateral autologous cultivated limbal epithelial transplantation.
abstract: OBJECTIVE
  To report the outcomes of autologous cultivated limbal epithelial transplantation using the healthy part of the affected eye or the fellow eye as a source of limbal stem cells in patients with unilateral, partial limbal stem cell deficiency (LSCD).
  
  
  METHODS
  Retrospective, nonrandomized, interventional case series.
  
  
  METHODS
  setting: L. V. Prasad Eye Institute, Hyderabad, India. study population: Patients with unilateral, partial LSCD who underwent autologous cultivated limbal epithelial transplantation between 2001 and 2011. intervention: The limbal biopsy was taken either from the healthy part of the limbus of the same eye (ipsilateral group) or from the healthy fellow eye (contralateral group). Cells were cultivated using a xeno-free explant culture technique, and cultivated cells were transplanted onto the affected surface. primary outcome measure: Success of cultivated limbal epithelial transplantation, defined as a completely epithelialized, avascular, and clinically stable corneal surface.
  
  
  RESULTS
  Seventy eyes of 70 patients were studied. The mean follow up was 17.5 ± 7 months. In 34 eyes the limbal biopsy was taken from the ipsilateral eye and in the remaining 36 eyes from the contralateral eye. Clinical success was achieved in 70.59% of eyes in the ipsilateral group and 75% of eyes in the contralateral group (P = .79). Limbal transplant survival rates at the final follow-up visit were 65.1% ± 0.09% in the ipsilateral group and 53.6% ± 0.12% in the contralateral group (P = .74).
  
  
  CONCLUSIONS
  Ocular surface restoration in partial LSCD is possible with cell-based therapy. Outcomes are similar irrespective of whether the limbal biopsy is taken from the healthy part of the ipsilateral eye or the contralateral eye.
authors.affiliation: Cornea and Anterior Segment Services, L. V. Prasad Eye Institute, Hyderabad, India.
authors.forename: Jayesh
authors.initials: J
authors.lastname: Vazirani
authors.affiliation: Cornea and Anterior Segment Services, L. V. Prasad Eye Institute, Hyderabad, India; Sudhakar and Sreekanth Ravi Stem Cell Biology Laboratory, L. V. Prasad Eye Institute, Hyderabad, India.
authors.forename: Sayan
authors.initials: S
authors.lastname: Basu
authors.affiliation: Cornea and Anterior Segment Services, L. V. Prasad Eye Institute, Hyderabad, India.
authors.forename: Hemal
authors.initials: H
authors.lastname: Kenia
authors.affiliation: Department of Clinical Epidemiology and Biostatistics, L. V. Prasad Eye Institute, Hyderabad, India.
authors.forename: Md Hasnat
authors.initials: MH
authors.lastname: Ali
authors.affiliation: Sudhakar and Sreekanth Ravi Stem Cell Biology Laboratory, L. V. Prasad Eye Institute, Hyderabad, India.
authors.forename: Santhosh
authors.initials: S
authors.lastname: Kacham
authors.affiliation: Sudhakar and Sreekanth Ravi Stem Cell Biology Laboratory, L. V. Prasad Eye Institute, Hyderabad, India.
authors.forename: Indumathi
authors.initials: I
authors.lastname: Mariappan
authors.affiliation: Cornea and Anterior Segment Services, L. V. Prasad Eye Institute, Hyderabad, India; Sudhakar and Sreekanth Ravi Stem Cell Biology Laboratory, L. V. Prasad Eye Institute, Hyderabad, India. Electronic address: vsangwan@lvpei.org.
authors.forename: Virender
authors.initials: V
authors.lastname: Sangwan
mesh_terms: D002057:Burns, Chemical / Q000601:surgery; D002470:Cell Survival; D002478:Cells, Cultured; D003316:Corneal Diseases / Q000473:pathology / Q000601:surgery*; D004847:Epithelial Cells / Q000637:transplantation*; D019573:Epithelium, Corneal / Q000166:cytology*; D005126:Eye Burns / Q000139:chemically induced; D005260:Female; D006085:Graft Survival / Q000502:physiology; D006801:Humans; D016850:Limbus Corneae / Q000166:cytology*; D008297:Male; D012189:Retrospective Studies; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000473:pathology*; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D014792:Visual Acuity / Q000502:physiology; D055815:Young Adult