title: Gently does it for submicron crystals.
abstract: A protein structure has been refined with electron diffraction data obtained by using a very weak electron beam to collect large numbers of diffraction patterns from a few sub-micron-sized three-dimensional crystals.
authors.affiliation: Oliver B Zeldin is in the Department of Molecular and Cellular Physiology and the Howard Hughes Medical Institute, Stanford University, Stanford, United States zeldin@stanford.edu.
authors.forename: Oliver B
authors.initials: OB
authors.lastname: Zeldin
authors.forename: Axel T
authors.initials: AT
authors.lastname: Brunger
mesh_terms: D018360:Crystallography, X-Ray / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins
keywords: electron cryomicroscopy (cryo EM); electron crystallography; electron diffraction; method development; protein structure