title: Piperine production by endophytic fungus Colletotrichum gloeosporioides isolated from Piper nigrum.
abstract: Many endophytic fungi have been reported with the biosynthetic potential to produce same or similar metabolites present in host plants. The adaptations that might have acquired by these fungi as a result of the long-term association with their host plants can be the possible basis of their biosynthetic potential. The bioactive compounds originated from endophytes are currently explored for their potential applications in pharmaceutical, agriculture and food industries. Piper nigrum, a plant of the Piperaceae is very remarkable because of the presence of the alkaloid piperine. Piperine has been reported to have broad bioactive properties ranging from antimicrobial, antidepressant, anti-inflammatory, antioxidative to anticancer activities. Interestingly, piperine also plays a vital role in increasing the bioavailability of many drugs which again is a promising property. The current study was carried out to identify piperine producing endophytic fungus from Piper nigrum L. By screening various endophytic fungi, the isolate which was identified as member of Colletotrichum gloeosporioides was found to have the ability to form piperine and was confirmed by HPLC and LCMS. Considering the broad bioactive potential of piperine, the piperine producing fungi identified in the study can expect to have much industrial potential.
authors.affiliation: School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India.
authors.forename: S
authors.initials: S
authors.lastname: Chithra
authors.affiliation: School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India.
authors.forename: B
authors.initials: B
authors.lastname: Jasim
authors.affiliation: Regional Forensic Laboratory, Kannur, Kerala.
authors.forename: P
authors.initials: P
authors.lastname: Sachidanandan
authors.affiliation: School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India.
authors.forename: M
authors.initials: M
authors.lastname: Jyothis
authors.affiliation: School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India. Electronic address: radhakrishnanek@mgu.ac.in.
authors.forename: E K
authors.initials: EK
authors.lastname: Radhakrishnan
mesh_terms: D000470:Alkaloids / Q000096:biosynthesis*; D052117:Benzodioxoles; D002851:Chromatography, High Pressure Liquid; D020231:Colletotrichum / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D060026:Endophytes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D029222:Piper nigrum / Q000382:microbiology*; D010880:Piperidines; D053284:Polyunsaturated Alkamides
chemical_list: D000470:Alkaloids; D052117:Benzodioxoles; D010880:Piperidines; D053284:Polyunsaturated Alkamides; C008922:piperine
keywords: Colletotrichum gloeosporioides; Endophytic fungi; LCMS; Piper nigrum; Piperine