title: Development and validation of a sensitive LC-MS/MS method for the determination of D-serine in human plasma.
abstract: A validated LC-MS/MS method was developed for the determination of d -Serine in human plasma. The method was fully validated for use with human plasma samples and was linear from 0.19 nmol/ml to 25 nmol/ml. The coefficient of variation was ≤5% for the high QC standards and ≤8% for the low QC standards in plasma. d -Serine and l -serine were resolved by pre-column derivatization using (R)-1-Boc-2-piperidine carbonyl chloride as the derivatizating agent. The method was used to determine the concentration of d-serine in plasma samples obtained in patients receiving a continuous 5-day intravenous infusion of (R,S)-ketamine. The changes in d-Ser levels varied in the six patients, with circulating d-Ser levels increasing as much as 35% in a patient, while decreasing 20% in a patient. While only preliminary data, the results suggests the potential importance in determining the d-Ser levels in plasma and their potential role in physiological response.
authors.affiliation: Laboratory of Clinical Investigation, Division of Intramural Research Programs, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA; Department of Pharmacology, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, PR China.
authors.forename: Y
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Neurology, Drexel University College of Medicine, 245 North 15th Street, Philadelphia, PA 19102l, USA.
authors.forename: G M
authors.initials: GM
authors.lastname: Alexander
authors.affiliation: Department of Neurology, Drexel University College of Medicine, 245 North 15th Street, Philadelphia, PA 19102l, USA.
authors.forename: R J
authors.initials: RJ
authors.lastname: Schwartzman
authors.affiliation: Laboratory of Clinical Investigation, Division of Intramural Research Programs, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
authors.lastname: N Singh
authors.affiliation: Cooper Medical School of Rowan University, Camden, NJ 08103, USA.
authors.forename: M C
authors.initials: MC
authors.lastname: Torjman
authors.affiliation: Cooper Medical School of Rowan University, Camden, NJ 08103, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Goldberg
authors.affiliation: Laboratory of Clinical Investigation, Division of Intramural Research Programs, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA; Cooper Medical School of Rowan University, Camden, NJ 08103, USA.
authors.forename: I W
authors.initials: IW
authors.lastname: Wainer
authors.affiliation: Laboratory of Clinical Investigation, Division of Intramural Research Programs, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA. Electronic address: moaddelru@mail.nih.gov.
authors.forename: R
authors.initials: R
authors.lastname: Moaddel
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D010949:Plasma / Q000737:chemistry*; D012694:Serine / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D012694:Serine
keywords: Chiral derivatization; Complex regional pain syndrome (CRPS); Enantiomeric separation; Ketamine