title: In vivo labeling of peroxisomes by photoconvertible mEos2 in myelinating glia of mice.
abstract: Mutations of several genes encoding peroxisomal proteins have been associated with human diseases. Some of these display specific white matter abnormalities in the brain, although the affected proteins are ubiquitously expressed. To better understand the etiology of peroxisomal myelin diseases, we aimed to label these organelles in vivo and in a cell type specific fashion. We had previously shown that in oligodendrocytes and Schwann cells numerous peroxisomes reside in the cytoplasmic channels of "non-compacted" myelin. These organelles are smaller and biochemically distinct from non-myelin peroxisomes. Targeting peroxisomal functions in various cell types of the brain has demonstrated that oligodendroglial peroxisomes are specifically important for long-term integrity of the CNS. To visualize myelin peroxisomes in intact cells and tissues by live imaging, we have generated a novel line of transgenic mice for the expression of fluorescently tagged peroxisomes specifically in myelinating glia. This was achieved by modifying the gene for a photoconvertible mEos2 with a peroxisomal targeting signal type 1 (PTS1) and generating a fusion gene with the myelin-specific Cnp1 promoter. In the brain of resulting transgenic mice, peroxisomes are selectively labeled in oligodendrocytes. In this novel genetic tool, photoconversion of single peroxisomes from green to red fluorescence can be used to monitor the fate of single organelles and to determine the dynamics of PTS1-mediated protein import in the context of myelin diseases that affect peroxisomal functions.
authors.affiliation: Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Hermann-Rein-Straße 3, 37075 Göttingen, Germany. Electronic address: Richert@em.mpg.de.
authors.forename: Sarah
authors.initials: S
authors.lastname: Richert
authors.affiliation: Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Hermann-Rein-Straße 3, 37075 Göttingen, Germany. Electronic address: Kleinecke@em.mpg.de.
authors.forename: Sandra
authors.initials: S
authors.lastname: Kleinecke
authors.affiliation: Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Hermann-Rein-Straße 3, 37075 Göttingen, Germany. Electronic address: Barth@em.mpg.de.
authors.forename: Jenniffer
authors.initials: J
authors.lastname: Günther
authors.affiliation: Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Hermann-Rein-Straße 3, 37075 Göttingen, Germany. Electronic address: Schaumburg@em.mpg.de.
authors.forename: Florian
authors.initials: F
authors.lastname: Schaumburg
authors.affiliation: Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Hermann-Rein-Straße 3, 37075 Göttingen, Germany. Electronic address: Edgar@em.mpg.de.
authors.forename: Julia
authors.initials: J
authors.lastname: Edgar
authors.affiliation: Institute of Applied Physics (APH) and Institute of Toxicology and Genetics (ITG), Karlsruhe Institute of Technology (KIT), Wolfgang-Gaede-Strasse 1, 76131 Karlsruhe, Germany. Electronic address: Uli.Nienhaus@kit.edu.
authors.forename: Gerd Ulrich
authors.initials: GU
authors.lastname: Nienhaus
authors.affiliation: Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Hermann-Rein-Straße 3, 37075 Göttingen, Germany. Electronic address: Nave@em.mpg.de.
authors.forename: Klaus-Armin
authors.initials: KA
authors.lastname: Nave
authors.affiliation: Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Hermann-Rein-Straße 3, 37075 Göttingen, Germany. Electronic address: Kassmann@em.mpg.de.
authors.forename: Celia M
authors.initials: CM
authors.lastname: Kassmann
mesh_terms: D000818:Animals; D051379:Mice; D008822:Mice, Transgenic; D009186:Myelin Sheath / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism*; D009836:Oligodendroglia / Q000378:metabolism*; D020675:Peroxisomes / Q000378:metabolism*
keywords: Fluorescent peroxisomes; Oligodendrocytes; Photoconversion; Transgenic mouse; mEos2