title: Hoechst 33258 as a vital stain for plant cell protoplasts.
abstract: A method for staining nuclei in unfixed protoplasts using Hoechst 33258 is described. It was found that the dye was not taken up by all protoplasts except in the presence of a detergent and that the staining of the nuclei was pH- and concentration-dependent. The use of vital fluorescent probes with plant protoplasts may be helpful for the identification, selection and subsequent culture of hybrid fusion products in automatic cell sorting systems.
authors.affiliation: Friedrich Miescher-Institut, Postfach 273, CH-4002, Basel, Switzerland.
authors.forename: M G
authors.initials: MG
authors.lastname: Meadows
authors.forename: I
authors.initials: I
authors.lastname: Potrykus