title: ISG15 is counteracted by vaccinia virus E3 protein and controls the proinflammatory response against viral infection.
abstract: Conjugation of ISG15 inhibits replication of several viruses. Here, using an expression system for assaying human and mouse ISG15 conjugations (ISGylations), we have demonstrated that vaccinia virus E3 protein binds and antagonizes human and mouse ISG15 modification. To study ISGylation importance in poxvirus infection, we used a mouse model that expresses deconjugating proteases. Our results indicate that ISGylation restricts in vitro replication of the vaccinia virus VVΔE3L mutant but unconjugated ISG15 is crucial to counteract the inflammatory response produced after VVΔE3L infection.
authors.affiliation: Department of Preventive Medicine and Public Health and Microbiology, Universidad Autónoma, Madrid, Spain.
authors.forename: Benedito
authors.initials: B
authors.lastname: Eduardo-Correia
authors.forename: Carles
authors.initials: C
authors.lastname: Martínez-Romero
authors.forename: Adolfo
authors.initials: A
authors.lastname: García-Sastre
authors.forename: Susana
authors.initials: S
authors.lastname: Guerra
mesh_terms: D000818:Animals; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006652:Histological Techniques; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation / Q000235:genetics; D010447:Peptide Hydrolases / Q000378:metabolism; D011213:Poxviridae Infections / Q000378:metabolism* / Q000473:pathology*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D014452:Ubiquitins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication / Q000235:genetics*
chemical_list: D016207:Cytokines; C081374:E3L protein, Vaccinia virus; C402395:HERC5 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D016601:RNA-Binding Proteins; D014452:Ubiquitins; D014764:Viral Proteins; C074034:ISG15 protein, human; D010447:Peptide Hydrolases