title: Exopolymers from Tolypothrix tenuis and three Anabaena sp. (Cyanobacteriaceae) as novel blood clotting agents for wound management.
abstract: Rapid initiation of clotting is critical to trauma patients. In the present study exopolymers (EPs) from four desert cyanobacteria including Tolypothrix tenuis and three species of Anabaena have been discovered as potential hemostatic biomaterials. The EPs showed reduction in activated partial thromboplastin time (APTT) and prothrombin time (PT) by 16-41% and 12-65%, respectively. Besides hastening blood clotting, the EPs could absorb 7.1-25.9 g H₂O g(-1) EP and displayed 7.1-18.1% hydrophobicity. They were noncytotoxic and biodegradable. The EP from Anabaena sp. showed strong antibacterial activity against E. coli, S. aureus and B. licheniformis. These results suggest that cyanobacteria, the microscopic phototrophs growing rapidly over simple mineral medium could prove to be a novel source of affordable hemostatic dressings for the traumatic wounds in underdeveloped and developing countries. Compositional analysis of the EPs showed them to be consisting of mainly carbohydrate (17-50%), protein (4.4-7.2%), uronic acid (4.7-9.5%) and sulphate (0.6-6.6%). Their viscometric molecular weight ranged from 539 to 3679 kDa. They were further characterized using GC-MS and FTIR.
authors.affiliation: Algae Biofuel and Biomolecules Centre, Department of Microbiology, M.D.S. University, Ajmer, 305009 Rajasthan, India. Electronic address: monicaajmer@gmail.com.
authors.forename: Monica
authors.initials: M
authors.lastname: Bhatnagar
authors.forename: Laxmi
authors.initials: L
authors.lastname: Parwani
authors.forename: Vinay
authors.initials: V
authors.lastname: Sharma
authors.forename: Jhuma
authors.initials: J
authors.lastname: Ganguly
authors.forename: Ashish
authors.initials: A
authors.lastname: Bhatnagar
mesh_terms: D017033:Anabaena / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001407:Bacillus / Q000187:drug effects / Q000254:growth & development; D001672:Biocompatible Materials / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects*; D000458:Cyanobacteria / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D008401:Gas Chromatography-Mass Spectrometry; D006490:Hemostatics / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D010314:Partial Thromboplastin Time; D011108:Polymers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011517:Prothrombin Time; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D006490:Hemostatics; D011108:Polymers
keywords: Antibacterial; Biodegradable; Cyanobacteria; Cytotoxicity; Exopolymers; Thrombogenic; Wound healing