title: Classification and expression diversification of wheat dehydrin genes.
abstract: Dehydrins (DHNs) are late embryonic abundant proteins characterized by the dehydrin domains that are involved in plant abiotic stress tolerance. In this study, fifty-four wheat DHN unigenes were identified in the expressed sequence tags database. These genes encode seven types of dehydrins (KS, SK3, YSK2, Y2SK2, Kn, Y2SK3, and YSK3) and separate in 32 homologous clusters. The gene amplification differed among the dehydrin types, and members of the YSK2- and Kn-type DHNs are more numerous in wheat than in other cereals. The relative expression of all of these DHN clusters was analyzed using an in silico method in seven tissue types (i.e. normal growing shoots, roots, and reproductive tissues; developing and germinating seeds; drought- and cold-stressed shoots) as well as semi-quantitative reverse transcription polymerase chain reaction in seedling leaves and roots treated by dehydration, cold, and salt, respectively. The role of the ABA pathway in wheat DHN expression regulation was analyzed. Transcripts of certain types of DHNs accumulated specifically according to tissue type and treatment, which suggests their differentiated roles in wheat abiotic stress tolerance.
authors.affiliation: The Applied Plant Genomics Lab, Crop Genomics and Bioinformatics Center & National Key Lab of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Jiangsu 210095, China; Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang Province 310021 China.
authors.forename: Yuezhi
authors.initials: Y
authors.lastname: Wang
authors.forename: Haibin
authors.initials: H
authors.lastname: Xu
authors.forename: Huilan
authors.initials: H
authors.lastname: Zhu
authors.forename: Ye
authors.initials: Y
authors.lastname: Tao
authors.forename: Guangxiang
authors.initials: G
authors.lastname: Zhang
authors.forename: Lixia
authors.initials: L
authors.lastname: Zhang
authors.forename: Caiqin
authors.initials: C
authors.lastname: Zhang
authors.forename: Zhengzhi
authors.initials: Z
authors.lastname: Zhang
authors.forename: Zhengqiang
authors.initials: Z
authors.lastname: Ma
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D003080:Cold Temperature; D055864:Droughts; D020224:Expressed Sequence Tags; D035264:Flowers / Q000235:genetics / Q000254:growth & development; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D014644:Genetic Variation; D005810:Multigene Family; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D010940:Plant Proteins / Q000145:classification / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000235:genetics / Q000254:growth & development; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D012965:Sodium Chloride / Q000494:pharmacology; D014908:Triticum / Q000235:genetics* / Q000254:growth & development
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins; C070556:dehydrin proteins, plant; D012965:Sodium Chloride; D000040:Abscisic Acid
keywords: Abiotic stress; Cereal; Dehydrin; Expression diversification; Gene duplication; Wheat