title: The chalcone synthase multigene family of Petunia hybrida (V30): sequence homology, chromosomal localization and evolutionary aspects.
abstract: Chalcone synthase (CHS) genes in Petunia hybrida comprise a multigene family containing at least 7 complete members in the strain Violet 30 (V30). Based on a high sequence homology in both coding and non-coding sequence, a number of CHS genes can be placed into two subfamilies. By restriction fragment length polymorphism (RFLP) analysis it was shown that both chromosomes II and V carry one of these subfamilies, in addition to the other CHS genes identified so far. Members of a subfamily were found to be closely linked genetically. Analysis of the Petunia species that contributed to the hybrid nature of P. hybrida (P. axillaris, P. parodii, P. inflata and P. violacea) shows that none of the CHS gene clusters is specific for either one of the parents and therefore did not arise as a consequence of the hybridization. The number of CHS genes within a subfamily varies considerably among these Petunia species. From this we infer that the CHS subfamilies arose from very recent gene duplications.
authors.affiliation: Department of Applied Genetics, Free University, De Boelelaan 1087, 1081 HV, Amsterdam, Netherlands.
authors.forename: R E
authors.initials: RE
authors.lastname: Koes
authors.forename: C E
authors.initials: CE
authors.lastname: Spelt
authors.forename: J N
authors.initials: JN
authors.lastname: Mol
authors.forename: A G
authors.initials: AG
authors.lastname: Gerats