title: Discovery of genes related to witches broom disease in Paulownia tomentosa × Paulownia fortunei by a De Novo assembled transcriptome.
abstract: In spite of its economic importance, very little molecular genetics and genomic research has been targeted at the family Paulownia spp. The little genetic information on this plant is a big obstacle to studying the mechanisms of its ability to resist Paulownia Witches' Broom (PaWB) disease. Analysis of the Paulownia transcriptome and its expression profile data are essential to extending the genetic resources on this species, thus will greatly improves our studies on Paulownia. In the current study, we performed the de novo assembly of a transcriptome on P. tomentosa × P. fortunei using the short-read sequencing technology (Illumina). 203,664 unigenes with a mean length of 1,328 bp was obtained. Of these unigenes, 32,976 (30% of all unigenes) containing complete structures were chosen. Eukaryotic clusters of orthologous groups, gene orthology, and the Kyoto Encyclopedia of Genes and Genomes annotations were performed of these unigenes. Genes related to PaWB disease resistance were analyzed in detail. To our knowledge, this is the first study to elucidate the genetic makeup of Paulownia. This transcriptome provides a quick way to understanding Paulownia, increases the number of gene sequences available for further functional genomics studies and provides clues to the identification of potential PaWB disease resistance genes. This study has provided a comprehensive insight into gene expression profiles at different states, which facilitates the study of each gene's roles in the developmental process and in PaWB disease resistance.
authors.affiliation: Institute of Paulownia, Henan Agricultural University, Zhengzhou, Henan, P. R. China ; College of Forestry, Henan Agricultural University, Zhengzhou, Henan, P. R. China.
authors.forename: Rongning
authors.initials: R
authors.lastname: Liu
authors.forename: Yanpeng
authors.initials: Y
authors.lastname: Dong
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Fan
authors.forename: Zhenli
authors.initials: Z
authors.lastname: Zhao
authors.forename: Minjie
authors.initials: M
authors.lastname: Deng
authors.forename: Xibing
authors.initials: X
authors.lastname: Cao
authors.forename: Suyan
authors.initials: S
authors.lastname: Niu
mesh_terms: D001483:Base Sequence; D016000:Cluster Analysis; D017931:DNA Primers; D020869:Gene Expression Profiling; D017343:Genes, Plant; D019684:Magnoliopsida / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D017931:DNA Primers