title: Understanding non-coding DNA regions in yeast.
abstract: Non-coding transcripts play an important role in gene expression regulation in all species, including budding and fission yeast. Such regulatory transcripts include intergenic ncRNA (non-coding RNA), 5' and 3' UTRs, introns and antisense transcripts. In the present review, we discuss advantages and limitations of recently developed sequencing techniques, such as ESTs, DNA microarrays, RNA-Seq (RNA sequencing), DRS (direct RNA sequencing) and TIF-Seq (transcript isoform sequencing). We provide an overview of methods applied in yeast and how each of them has contributed to our knowledge of gene expression regulation and transcription.
authors.affiliation: *Mathematical Institute, University of Oxford, Oxford OX1 3LB, U.K.
authors.forename: Margarita
authors.initials: M
authors.lastname: Schlackow
authors.forename: Monika
authors.initials: M
authors.lastname: Gullerova
mesh_terms: D004271:DNA, Fungal / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D022661:RNA, Untranslated / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D004271:DNA, Fungal; D022661:RNA, Untranslated