title: Shoot regeneration from mesophyll protoplasts and leaf explants of Rehmannia glutinosa.
abstract: Mesophyll protoplasts obtained from leaves of shoot cultures of Rehmannia glutinosa were cultured in Murashige and Skoog (1962) liquid or liquid-over-agar medium containing 2.0 mg L(-1) naphthaleneacetic acid and 0.5 mg L(-1) benzylamino purine. An amino acid mixture of glutamine, arginine, glycine, and aspartic acid promoted sustained protoplast division, with an average plating efficiency of 27%. Protoplast-derived colonies formed callus which readily regenerated shoots on fransfer to Murashige and Skoog based agar medium with 2.0 mg L(-1) indoleacetic acid and 1.0 mg L(-1) benzylamino purine. Leaf explants also showed a marked capacity for shoot regeneration in culture.
authors.affiliation: Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, University Park, Nottingham, UK.
authors.forename: X H
authors.initials: XH
authors.lastname: Xu
authors.forename: M R
authors.initials: MR
authors.lastname: Davey