title: CAP1 was associated with actin and involved in Schwann cell differentiation and motility after sciatic nerve injury.
abstract: Adenylate cyclase-associated protein 1 (CAP1), a member of cyclase-associated proteins that regulating actin dynamics, was shown to regulate actin filaments, localize to dynamic actin structures and mediate such processes as establishment of cell polarity, motility, morphogenesis, receptor-mediated endocytosis and mRNA location. But little is known about the role of CAP1 during peripheral nervous system injury. Here, we found the spatiotemporal protein expression of CAP1 after sciatic nerve crush. After crush, CAP1 had an increased protein expression level, reached a peak at about day 5 and then returned to the normal level at 4 weeks, similar to Oct-6. Besides, in 5-day injured tissue, using double immunofluorescent staining we found CAP1 had a colocalization with S100 and Oct-6. In vitro, during the process of cAMP-induced Schwann cells differentiation, we observed enhanced expression of CAP1 and P0. Specially, CAP1-specific siRNA-tranfected SCs did not show significant actin structure which form cellure surface tension and protrusion shape after cAMP treatment. And we observed the interaction of CAP1 with actin and that CAP1-specific siRNA-transfected SCs had a decreased motility and migration. Together, all these data indicated that the change of CAP1 protein expression was associated with Schwann cells motility and differentiation after the crush of sciatic nerve.
authors.affiliation: Department of Orthopaedics, The Second Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Xinhui
authors.initials: X
authors.lastname: Zhu
authors.forename: Li
authors.initials: L
authors.lastname: Yao
authors.forename: Aisong
authors.initials: A
authors.lastname: Guo
authors.forename: Aihong
authors.initials: A
authors.lastname: Li
authors.forename: Huiqing
authors.initials: H
authors.lastname: Sun
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Hanzhang
authors.initials: H
authors.lastname: Liu
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Duan
authors.forename: Jianhua
authors.initials: J
authors.lastname: Cao
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement; D000242:Cyclic AMP / Q000378:metabolism / Q000494:pharmacology; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D015870:Gene Expression; D005786:Gene Expression Regulation; D008297:Male; D027701:Organic Cation Transport Proteins / Q000378:metabolism; D059348:Peripheral Nerve Injuries / Q000378:metabolism* / Q000473:pathology; D011485:Protein Binding; D021381:Protein Transport; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012583:Schwann Cells / Q000166:cytology* / Q000378:metabolism*; D012584:Sciatic Nerve / Q000293:injuries* / Q000378:metabolism*
chemical_list: D000199:Actins; C081782:Cap1 protein, rat; D003598:Cytoskeletal Proteins; D027701:Organic Cation Transport Proteins; D034741:RNA, Small Interfering; D000242:Cyclic AMP