title: Two replication-defective adenoviral vaccine vectors for the induction of immune responses to PPRV.
abstract: Peste des petits ruminants is a highly contagious disease of small ruminants caused by a Morbillivirus, peste des petits ruminants virus (PPRV). Two recombinant replication-defective human adenovirus serotype 5 (Ad5) containing the highly immunogenic fusion protein (F) and hemaglutinine protein (H) genes from PPRV were constructed. HEK293A cells infected with either virus (Ad5-PPRV-F or -H) express F and H proteins respectively. These viruses were used to vaccinate mice by intramuscular inoculation. Both viruses elicited PPRV-specific B- and T-cell responses. Thus, after two immunizations, sera from immunized mice elicited neutralizing antibody response, indicating that this approach has the potential to confer protective immunity. In addition, we detected a significant antigen specific CD4(+) and CD8(+) T-cell response in mice vaccinated with either virus. These results indicate that these adenovirus constructs offer a promising alternative to current vaccine strategies for the development of PPRV DIVA vaccines.
authors.affiliation: Centro de Investigación en Sanidad Animal (CISA-INIA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28130 Valdeolmos, Madrid, Spain. Electronic address: jmrojas@cnb.csic.es.
authors.forename: José M
authors.initials: JM
authors.lastname: Rojas
authors.affiliation: Centro de Investigación en Sanidad Animal (CISA-INIA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28130 Valdeolmos, Madrid, Spain. Electronic address: hector.moreno@inia.es.
authors.forename: Héctor
authors.initials: H
authors.lastname: Moreno
authors.affiliation: Centro Nacional de Investigaciones Cardiovasculares (CNIC), C/Melchor Fernández Almagro no 3, 28029 Madrid, Spain. Electronic address: agarcia@cnic.es.
authors.forename: Aída
authors.initials: A
authors.lastname: García
authors.affiliation: Centro Nacional de Investigaciones Cardiovasculares (CNIC), C/Melchor Fernández Almagro no 3, 28029 Madrid, Spain. Electronic address: jcramirez@cnic.es.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Ramírez
authors.affiliation: Centro de Investigación en Sanidad Animal (CISA-INIA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28130 Valdeolmos, Madrid, Spain. Electronic address: sevilla@inia.es.
authors.forename: Noemí
authors.initials: N
authors.lastname: Sevilla
authors.affiliation: Centro de Investigación en Sanidad Animal (CISA-INIA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28130 Valdeolmos, Madrid, Spain. Electronic address: veronica.martin@inia.es.
authors.forename: Verónica
authors.initials: V
authors.lastname: Martín
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D001402:B-Lymphocytes / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002460:Cell Line; D004337:Drug Carriers; D005260:Female; D006801:Humans; D007273:Injections, Intramuscular; D051379:Mice; D008810:Mice, Inbred C57BL; D029021:Peste-des-Petits-Ruminants / Q000517:prevention & control / Q000662:veterinary*; D018107:Peste-des-petits-ruminants virus / Q000235:genetics / Q000276:immunology*; D014611:Vaccination / Q000379:methods; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D004337:Drug Carriers; D014614:Vaccines, Synthetic; D014765:Viral Vaccines
keywords: Adenoviral vectors; Immune response; PPRV; Vaccines