title: Cycline efficacy on the propagation of human prions in primary cultured neurons is strain-specific.
abstract: In prion diseases, a major issue in therapeutic research is the variability of the effect between strains. Stimulated by the report of an antiprion effect in a scrapie model and by ongoing international clinical trials using doxycycline, we studied the efficacy of cyclines against the propagation of human prions. First, we successfully propagated various Creutzfeldt-Jakob disease (CJD) isolates (sporadic, variant, and iatrogenic CJD) in neuronal cultures expressing the human prion protein. Then, we found that doxycycline was the most effective compound, with important variations between isolates. Isolates from sporadic CJD, the most common form of prion disease, showed the highest sensitivity.
authors.affiliation: Université Pierre et Marie Curie - Paris 6, Centre de Recherche de l'Institut du Cerveau et de la Moelle épinière (CRICM), UMRS 975, Equipe "Alzheimer's and Prion Diseases"
authors.forename: Samia
authors.initials: S
authors.lastname: Hannaoui
authors.forename: Alexianne
authors.initials: A
authors.lastname: Gougerot
authors.forename: Nicolas
authors.initials: N
authors.lastname: Privat
authors.forename: Etienne
authors.initials: E
authors.lastname: Levavasseur
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bizat
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Hauw
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Brandel
authors.forename: Stéphane
authors.initials: S
authors.lastname: Haïk
mesh_terms: D002478:Cells, Cultured; D004318:Doxycycline / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism*; D011328:Prions / Q000037:antagonists & inhibitors* / Q000187:drug effects*
chemical_list: D011328:Prions; D004318:Doxycycline
keywords: Creutzfeldt-Jakob disease; isolates; prion; strain; treatment