title: Partial Fractionation of Venoms from Two Iranian Vipers, Echis carinatus and Cerastes persicus Fieldi and Evaluation of Their Antiplatelet Activity.
abstract: Platelet aggregation inhibitory effect and anticoagulant properties of fractions separated from the venoms of Cerastes persicus fieldi and Echis carinatus were investigated. The partial fractionation was performed on a Sephadex G-100 column. Two fractions separated from Cerastes persicus fieldi showed anti platelet aggregation activity on ADP (200 μM)-induced platelet aggregation (ca 80% inhibition). Attempts to measure the antiplatelet aggregation activity of crude Echis carinatus venom and its fractions were not successful due to the protein coagulation of the plasma samples after the addition of venom. Anticoagulant activities of venoms were also evaluated. Total venom of Echis carinatus showed anti coagulant activity in PT test, while its fractions showed procoagulant activity.
authors.affiliation: Department of Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Toktam
authors.initials: T
authors.lastname: Mehdizadeh Kashani
authors.forename: Hossein
authors.initials: H
authors.lastname: Vatanpour
authors.forename: Hossein
authors.initials: H
authors.lastname: Zolfagharian
authors.forename: Hasan
authors.initials: H
authors.lastname: Hooshdar Tehrani
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Heydari
authors.forename: Farzad
authors.initials: F
authors.lastname: Kobarfard
keywords: Anticoagulant; Antiplatelet aggregation; Cerastes persicus fieldi; Echis carinatus; Gel filtration; Snake venom