title: Paenalcaligenes suwonensis sp. nov., isolated from spent mushroom compost.
abstract: A bacterial strain, ABC02-12(T), was isolated from spent mushroom compost, a waste product of button mushroom cultivation. Cells of the strain were Gram-stain-negative, catalase- and oxidase-positive, non-spore-forming, aerobic flagellated rods. Optimum growth occurred at 28 °C and pH 7.0. 16S rRNA gene sequence analysis showed that strain ABC02-12(T) shared the highest sequence similarities with Paenalcaligenes hominis CCUG 53761A(T) (96.0 %), Alcaligenes faecalis subsp. parafaecalis G(T) (95.7 %), Alcaligenes faecalis subsp. faecalis IAM 12369(T) (95.4 %) and Pusillimonas noertemannii BN9(T) (95.3 %). According to the phylogenetic tree, strain ABC02-12(T) formed a robust cluster with Paenalcaligenes hominis CCUG 53761A(T) and Paenalcaligenes hermetiae KBL009(T). The quinone system was ubiquinone Q-8 with minor amounts of Q-7. The major fatty acids (>5 % of total fatty acids) were C16 : 0, C16 : 1ω6c and/or C16 : 1ω7c (summed feature 3), C18 : 1ω7c and/or C18 : 1ω6c (summed feature 8), C17 : 0 cyclo, and iso-C16 : 1 I, C14 : 0 3-OH and/or an unknown fatty acid (summed feature 2). The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown aminolipid. Putrescine was the principal polyamine, with small amounts of 2-hydroxyputrescine and cadaverine. On the basis of the evidence presented in this study, strain ABC02-12(T) is a representative of a novel species within the genus Paenalcaligenes, for which the name Paenalcaligenes suwonensis sp. nov. is proposed. The type strain is ABC02-12(T) ( = KACC 16537(T) = NBRC 108927(T)).
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.|Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, Republic of Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Moon
authors.affiliation: Department of Microbiology, Dankook University, Anseo-dong, Cheonan 330-714, Republic of Korea.|Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, Republic of Korea.
authors.forename: Jun-Muk
authors.initials: JM
authors.lastname: Lim
authors.affiliation: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, Republic of Korea.
authors.forename: Jae-Hyung
authors.initials: JH
authors.lastname: Ahn
authors.affiliation: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, Republic of Korea.
authors.forename: Hang-Yeon
authors.initials: HY
authors.lastname: Weon
authors.affiliation: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, Republic of Korea.
authors.forename: Soon-Wo
authors.initials: SW
authors.lastname: Kwon
authors.affiliation: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, Republic of Korea.
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Kim
mesh_terms: D000363:Agaricales; D042401:Alcaligenaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010802:Phylogeny; D011073:Polyamines / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011073:Polyamines; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8