title: Somatic embryogenesis and plant regeneration in callus culture derived from immature seeds and mature zygotic embryos of Dysosma pleiantha (Hance) Woodson.
abstract: Callus was induced on the wounded immature seeds and mature zygotic embryos of Dysosma pleiantha (Hance) Woodson (Berberidaceae) on a medium based on Murashige and Skoog's (1962) formula supplemented with 1 mg/l 2,4-dichlorophenoxy-acetic acid (2,4-D). Spontaneous embryoid formation occurred on the media containing low concentrations of 2,4-D (0.1-0.5 mg/l). These embryoids germinated in either MS or B5 medium containing 1 mg/l N(6)-benzyladenine and 1 mg/l gibberellic acid. The regenerated plantlets were successfully transferred to soil.
authors.affiliation: Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.
authors.forename: M J
authors.initials: MJ
authors.lastname: Chuang
authors.forename: W C
authors.initials: WC
authors.lastname: Chang