title: Circulating microparticles from obstructive sleep apnea syndrome patients induce endothelin-mediated angiogenesis.
abstract: Microparticles are deemed true biomarkers and vectors of biological information between cells. Depending on their origin, the composition of microparticles varies and the subsequent message transported by them, such as proteins, mRNA, or miRNA, can differ. In obstructive sleep apnea syndrome (OSAS), circulating microparticles are associated with endothelial dysfunction by reducing endothelial-derived nitric oxide production. Here, we have analyzed the potential role of circulating microparticles from OSAS patients on the regulation of angiogenesis and the involved pathway. VEGF content carried by circulating microparticles from OSAS patients was increased when compared with microparticles from non-OSAS patients. Circulating microparticles from OSAS patients induced an increase of angiogenesis that was abolished in the presence of the antagonist of endothelin-1 receptor type B. In addition, endothelin-1 secretion was increased in human endothelial cells treated by OSAS microparticles. We highlight that circulating microparticles from OSAS patients can modify the secretome of endothelial cells leading to angiogenesis.
authors.affiliation: LUNAM Université, Angers, France; INSERM U1063, Angers, France.
authors.forename: Simon
authors.initials: S
authors.lastname: Tual-Chalot
authors.affiliation: LUNAM Université, Angers, France; INSERM U1063, Angers, France; Département de Pneumologie, CHU d'Angers, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Gagnadoux
authors.affiliation: LUNAM Université, Angers, France; INSERM U1063, Angers, France; Département de Pneumologie, CHU d'Angers, France.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Trzepizur
authors.affiliation: LUNAM Université, Angers, France; INSERM U1063, Angers, France; Département de Pneumologie, CHU d'Angers, France.
authors.forename: Pascaline
authors.initials: P
authors.lastname: Priou
authors.affiliation: LUNAM Université, Angers, France; INSERM U1063, Angers, France; CHU d'Angers, France.
authors.forename: Ramaroson
authors.initials: R
authors.lastname: Andriantsitohaina
authors.affiliation: LUNAM Université, Angers, France; INSERM U1063, Angers, France. Electronic address: carmen.martinez@univ-angers.fr.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Martinez
mesh_terms: D000328:Adult; D000368:Aged; D001011:Aorta / Q000166:cytology; D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D002448:Cell Adhesion / Q000502:physiology; D055252:Cell-Derived Microparticles / Q000378:metabolism / Q000502:physiology*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D065131:Endothelin B Receptor Antagonists; D019332:Endothelin-1 / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology*; D009842:Oligopeptides / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology; D044023:Receptor, Endothelin B / Q000378:metabolism*; D020181:Sleep Apnea, Obstructive / Q000097:blood / Q000503:physiopathology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism / Q000494:pharmacology; D055815:Young Adult
chemical_list: D065131:Endothelin B Receptor Antagonists; D019332:Endothelin-1; D009842:Oligopeptides; D010880:Piperidines; D044023:Receptor, Endothelin B; D042461:Vascular Endothelial Growth Factor A; C086539:BQ 788
keywords: Angiogenesis; Endothelin-1; Human endothelial cell; Microparticle; Obstructive sleep apnea syndrome