title: Selection and regeneration of toxin-insensitive plants from tissue cultures of oats (Avena sativa) susceptible to Helminthosporium victoriae.
abstract: Insensitivity to the pathotoxin victorin, which is produced by the fungus Helminthosporium victoriae (Meehan and Murphy), was selected in tissue cultures of oat (Avena sativa L.) lines heterozygous for the dominant sensitive allele Vb. The Vb allele imparts both susceptibility to H. victoriae and resistance to several races of oat crown rust (Puccinia coronata var. 'avenae', Fraser and E. Led.). None of 84 homozygous Vb Vb oat calli survived when grown on victorin-containing medium. Among 175 calli of heterozygous Vb vb cultures grown on toxin-containing medium, 16 representing 13 separate embryo-derived culture lines produced surviving callus sectors or shoots. Based on leaf bioassays of plants regenerated after toxin selection, nine culture lines gave toxin-insensitive plants and two gave plants showing the toxin sensitivity of the parent. Two selected lines failed to regenerate. Plants regenerated from 30 culture lines which had never been exposed to toxin-containing selection medium were all toxin sensitive. The toxin insensitivity of the regenerants from the toxin-selected culture lines was heritable since progeny of these plants were all insensitive. The toxin-insensitive selected lines all were found to have coincidentally lost the Vb crown rust resistance of the original line. In cytological analysis of meiotic cells of regenerants from the selected cultures, no chromosomal deficiency was found which could be associated with, and thus account for, the loss of sensitivity to the toxin. Somatic recombination and mutation to vb vb are other possible origins of toxin insensitivity in the selections. The victorin selection demonstrates that specific resistance can be selected in tissue cultures of oats. It also provides a highly sensitive scheme to test effects of culture conditions and chemical agents on induction of genetic and chromosomal changes in tissue cultures.
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Department of Agronomy and Plant Genetics, University of Minnesota, 55108, St. Paul, MN, USA.
authors.forename: H W
authors.initials: HW
authors.lastname: Rines
authors.forename: H H
authors.initials: HH
authors.lastname: Luke