title: Species discrimination in five species of winter-flying geometrids (Lepidoptera) based on chirality of semiochemicals and flight season.
abstract: Enantiomer separation of (6Z,9Z)-cis-3,4-epoxynonadecadiene and (3Z,9Z)-cis-6,7-epoxynonadecadiene could be achieved using chiral high-resolution gas chromatography and a cyclodextrin-bond column. (3Z,9Z)-(6R,7S)-Epoxynonadecadiene was identified from ovipositor extracts ofColotois pen-Naria, while inErannis defoliaria the 6S,7R-enantiomer was found. In field trapping tests pure synthetic enantiomers caught only conspecific males of these species. (3Z,6Z,9Z)-Nonadecatriene was found in both species, while the presence of (3Z,6Z,9Z)-heneicosatriene was indicated inC. Pennaria only. A 10∶10∶3 blend of (3Z,9Z)-(6R,7S)-epoxynonadecadiene, (3Z,6Z,9Z)-heneicosatriene, and (3Z,6Z,9Z)-nonadecatriene was found to be optimal for catchingC. Pennaria, whileE. Defoliaria males were optimally caught by a 1∶1 mixture of (3Z,9Z)-(6S,7R)-epoxynonadecadiene and (3Z,6Z,9Z)-nona-decatriene. (6Z,9Z)-(3S,4R)-Epoxynonadecadiene was identified from ovipositor extracts ofAgriopis (Erannis) aurantiaria. In field tests the pure enantiomer proved to be a highly specific sex attractant for both the late autumn/early winter flyingA. Aurantiaria and the late winter/early spring flyingA. Leucophearia. Males ofAgriopis marginaria, which fly in late winter/early spring, were attracted to (3Z,9Z)-(6S,7R)-epoxynonadecadiene. The addition of (3Z,6Z,9Z)-nonadecatriene to theS,R-enantiomer increased captures. Optimal catches were recorded with a 10∶3 epoxide-hydrocarbon blend. Enantiomer specificity in all species was confirmed in EAG measurements.
authors.affiliation: Plant Protection Institute of the Hungarian Academy of Sciences, H-1525, Budapest, Pf 102, Hungary.
authors.forename: G
authors.initials: G
authors.lastname: Szöcs
authors.forename: M
authors.initials: M
authors.lastname: Tóth
authors.forename: W
authors.initials: W
authors.lastname: Francke
authors.forename: F
authors.initials: F
authors.lastname: Schmidt
authors.forename: P
authors.initials: P
authors.lastname: Philipp
authors.forename: W A
authors.initials: WA
authors.lastname: König
authors.forename: K
authors.initials: K
authors.lastname: Mori
authors.forename: B S
authors.initials: BS
authors.lastname: Hansson
authors.forename: C
authors.initials: C
authors.lastname: Löfstedt