title: Carbon nanotubes based electrochemical aptasensing platform for the detection of hydroxylated polychlorinated biphenyl in human blood serum.
abstract: A novel strategy to sense target molecules in human blood serum is achieved by immobilizing aptamers (APTs) on multi-walled carbon nanotubes (MWCNT) modified electrodes. In this work, the aminated aptamer selected for hydroxylated polychlorinated biphenyl (OH-PCB) was covalently immobilized on the surface of the MWCNT-COOH modified glassy carbon electrode through amide linkage. The aptamers function as recognition probes for OH-PCB by the binding induced folding of the aptamer. The developed aptasensing device was characterized by electrochemical impedance spectroscopy (EIS), atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR). The aptasensor displayed excellent performance for OH-PCB detection with a linear range from 0.16 to 7.5 μM. The sensitivity of the developed aptasensing platform is improved (1×10(-8) M) compared to the published report (1×10(-6) M) for the determination of OH-PCB (Turner et al., 2007). The better performance of the sensor is due to the unique platform, i.e. the presence of APTs onto electrodes and the combination with nanomaterials. The aptamer density on the electrode surface was estimated by chronocoulometry and was found to be 1.4×10(13) molecules cm(-2). The validity of the method and applicability of the aptasensor was successfully evaluated by the detection of OH-PCB in a blood serum sample. The described approach for aptasensing opens up new perspectives in the field of biomonitoring providing a device with acceptable stability, high sensitivity, good accuracy and precision.
authors.affiliation: University of Antwerp, Department of Chemistry, Groenenborgerlaan 171, 2020 Antwerp, Belgium.
authors.forename: Sanaz
authors.initials: S
authors.lastname: Pilehvar
authors.affiliation: University of Antwerp, Department of Chemistry, Groenenborgerlaan 171, 2020 Antwerp, Belgium.
authors.forename: Jahangir
authors.initials: J
authors.lastname: Ahmad Rather
authors.affiliation: University of Antwerp, Department of Biology, Groenenborgerlaan 171, 2020 Antwerp, Belgium.
authors.forename: Freddy
authors.initials: F
authors.lastname: Dardenne
authors.affiliation: Institute for Agricultural and Fisheries Research, Ankerstraat 1, 8400 Ostend, Belgium.
authors.forename: Johan
authors.initials: J
authors.lastname: Robbens
authors.affiliation: University of Antwerp, Department of Biology, Groenenborgerlaan 171, 2020 Antwerp, Belgium.
authors.forename: Ronny
authors.initials: R
authors.lastname: Blust
authors.affiliation: University of Antwerp, Department of Chemistry, Groenenborgerlaan 171, 2020 Antwerp, Belgium. Electronic address: Karolien.DeWael@uantwerpen.be.
authors.forename: Karolien
authors.initials: K
authors.lastname: De Wael
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques; D004566:Electrodes; D006801:Humans; D006900:Hydroxylation; D057230:Limit of Detection; D018625:Microscopy, Atomic Force; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011078:Polychlorinated Biphenyls / Q000097:blood*
chemical_list: D052157:Aptamers, Nucleotide; D037742:Nanotubes, Carbon; D011078:Polychlorinated Biphenyls
keywords: Electrochemical aptasensor (APT-MWCNT/GCE); Hydroxylated PCB (OH-PCBs); Multi-walled carbon nanotubes (MWCNTs); Serum analysis