title: Biosynthesis of silver nanoparticles: Elucidation of prospective mechanism and therapeutic potential.
abstract: The synthesis of silver nanoparticles (AgNPs) was accomplished using Syzygium cumini fruit extract at room temperature. Various techniques were used to characterize the newly synthesized silver nanoparticles and their size was determined to be 10-15nm. Important findings of this study were the identification of biomolecules responsible for the synthesis of silver nanoparticles and elucidate the mechanism of biosynthesis. Flavonoids present in S. cumini were mainly responsible for the reduction and the stabilization of nanoparticles. The antioxidant properties of AgNPs were evaluated using various assays. The nanoparticles were also found to destroy Dalton lymphoma cell lines under in vitro condition. Silver nanoparticles (100μg/mL) decreased the viability of Dalton lymphoma (DL) cell lines up to 50%. The studies describing the biosynthesis of silver nanoparticles by fruit extract followed by the investigation of synthesis mechanism and anti-cancer activities may be useful for nanobiotechnology research opening a new arena in this field.
authors.affiliation: Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar 160062, Punjab, India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Mittal
authors.forename: Jayeeta
authors.initials: J
authors.lastname: Bhaumik
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kumar
authors.forename: Uttam Chand
authors.initials: UC
authors.lastname: Banerjee
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005419:Flavonoids / Q000737:chemistry / Q000276:immunology; D005638:Fruit / Q000737:chemistry; D006801:Humans; D020128:Inhibitory Concentration 50; D016399:Lymphoma, T-Cell; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D010084:Oxidation-Reduction; D010851:Picrates / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D027842:Syzygium / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D010851:Picrates; D010936:Plant Extracts; D012834:Silver; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Anti-cancer activity; Biological synthesis; Mechanism of nanoparticle biosynthesis; Nanoparticles; Silver