title: Effects of daidzein and kiwifruit on bone mineral density and equol production in ovariectomised rats.
abstract: In this study, we investigated the synergistic effects of daidzein (Dz) and kiwifruit on bone and equol production in ovariectomised (OVX) rats. Female Sprague-Dawley rats were randomly assigned to one of five groups: sham operated, OVX control, OVX fed 0.1% Dz-supplemented diet (OVX + Dz), OVX fed 0.1% Dz and green kiwifruit (GRK)-supplemented diet (OVX + Dz + GRK) and OVX fed 0.1% Dz and gold kiwifruit (GOK)-supplemented diet (OVX + Dz + GOK). There were no significant differences in whole body and femur bone mineral density (BMD) among groups at week 8. BMD in the OVX group significantly decreased at week 8; however, BMD in the OVX + Dz + GRK was not significantly different from baseline in the end of the study. However, supplementation with kiwifruit did not affect urinary equol concentrations, urinary ratios of equol to Dz and the composition of caecal microbiota. These results suggest that the combination of Dz and GRK may slightly reduce bone loss caused by oestrogen deficiency but does not affect equol production.
authors.affiliation: Department of Food Function and Labeling, National Institute of Health and Nutrition , Toyama, Shinjuku-ku, Tokyo , Japan and.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Tousen
authors.forename: Frances M
authors.initials: FM
authors.lastname: Wolber
authors.forename: Wei-Hang
authors.initials: WH
authors.lastname: Chua
authors.forename: Miki
authors.initials: M
authors.lastname: Tadaishi
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Ishimi
authors.forename: Marlena C
authors.initials: MC
authors.lastname: Kruger
mesh_terms: D029042:Actinidia; D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D002432:Cecum / Q000187:drug effects / Q000382:microbiology; D019587:Dietary Supplements; D004357:Drug Synergism; D060754:Equol / Q000096:biosynthesis*; D005260:Female; D005638:Fruit; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology* / Q000627:therapeutic use; D015663:Osteoporosis, Postmenopausal / Q000378:metabolism* / Q000517:prevention & control; D010052:Ovariectomy; D048789:Phytoestrogens / Q000494:pharmacology / Q000627:therapeutic use; D028321:Plant Preparations / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007529:Isoflavones; D048789:Phytoestrogens; D028321:Plant Preparations; D060754:Equol; C004742:daidzein