title: A multi-laboratory evaluation of a clinically-validated incurred quality control material for analysis of allergens in food.
abstract: A dessert matrix previously used for diagnosis of food allergies was incurred with pasteurised egg white or skimmed milk powder at 3, 6, 15 and 30 mg allergen protein per kg of dessert matrix and evaluated as a quality control material for allergen analysis in a multi-laboratory trial. Analysis was performed by immunoassay using five kits each for egg and milk (based on casein) and six 'other' milk kits (five based on β-lactoglobulin and one total milk). All kits detected allergen protein at the 3 mg kg(-1) level. Based on ISO criteria only one egg kit accurately determined egg protein at 3 mg kg(-1) (p=0.62) and one milk (casein) kit accurately determined milk at 6 (p=0.54) and 15 mg kg(-1) (p=0.83), against the target value. The milk "other" kits performed least well of all the kits assessed, giving the least precise analyses. The incurred dessert material had the characteristics required for a quality control material for allergen analysis.
authors.affiliation: Institute of Inflammation and Repair, Manchester Academic Health Sciences Centre, Manchester Institute of Biotechnology, University of Manchester, 131, Princess Street, Manchester M1 7DN, UK; Institute of Food Research, Norwich Research Park, Colney Lane, Norwich NR4 7UA, UK. Electronic address: Philip.johnson-2@manchester.ac.uk.
authors.forename: Phil E
authors.initials: PE
authors.lastname: Johnson
authors.forename: Neil M
authors.initials: NM
authors.lastname: Rigby
authors.forename: Jack R
authors.initials: JR
authors.lastname: Dainty
authors.forename: Alan R
authors.initials: AR
authors.lastname: Mackie
authors.forename: Ulrike U
authors.initials: UU
authors.lastname: Immer
authors.forename: Adrian
authors.initials: A
authors.lastname: Rogers
authors.forename: Pauline
authors.initials: P
authors.lastname: Titchener
authors.forename: Masahiro
authors.initials: M
authors.lastname: Shoji
authors.forename: Anne
authors.initials: A
authors.lastname: Ryan
authors.forename: Luis
authors.initials: L
authors.lastname: Mata
authors.forename: Helen
authors.initials: H
authors.lastname: Brown
authors.forename: Thomas
authors.initials: T
authors.lastname: Holzhauser
authors.forename: Valery
authors.initials: V
authors.lastname: Dumont
authors.forename: Jill A
authors.initials: JA
authors.lastname: Wykes
authors.forename: Michael
authors.initials: M
authors.lastname: Walker
authors.forename: Jon
authors.initials: J
authors.lastname: Griffin
authors.forename: Jane
authors.initials: J
authors.lastname: White
authors.forename: Glenn
authors.initials: G
authors.lastname: Taylor
authors.forename: Bert
authors.initials: B
authors.lastname: Popping
authors.forename: René
authors.initials: R
authors.lastname: Crevel
authors.forename: Sonia
authors.initials: S
authors.lastname: Miguel
authors.forename: Petra
authors.initials: P
authors.lastname: Lutter
authors.forename: Ferdelie
authors.initials: F
authors.lastname: Gaskin
authors.forename: Terry B
authors.initials: TB
authors.lastname: Koerner
authors.forename: Dean
authors.initials: D
authors.lastname: Clarke
authors.forename: Robin
authors.initials: R
authors.lastname: Sherlock
authors.forename: Andrew
authors.initials: A
authors.lastname: Flanagan
authors.forename: Chun-Han
authors.initials: CH
authors.lastname: Chan
authors.forename: E N Clare
authors.initials: EN
authors.lastname: Mills
mesh_terms: D000485:Allergens / Q000032:analysis* / Q000276:immunology; D000818:Animals; D002364:Caseins / Q000032:analysis / Q000276:immunology; D002417:Cattle; D002645:Chickens; D019411:Clinical Laboratory Techniques / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D004531:Eggs / Q000032:analysis*; D005512:Food Hypersensitivity / Q000517:prevention & control*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D008892:Milk / Q000737:chemistry* / Q000276:immunology; D011786:Quality Control
chemical_list: D000485:Allergens; D002364:Caseins
keywords: Allergen; ELISA; Egg; Incurred standard; Milk; Multi-centre trial