title: Systematic reference sample generation for multiplexed serological assays.
abstract: Quality controls of serological assays have to contain defined amounts of human antibodies specific for the targeted antigen. A prevailing issue for array-based antigen assays is that dozens of antigens are targeted within the same assay. Commonly different patient sera are combined and optimal pools are empirically identified. Here, we report a mathematical approach how an optimal sample pool composition can be systematically calculated and accurately compiled. The approach was used to compose suitable quality controls for a 71 plex Tuberculosis antigen bead array using a limited number of positive human sera.
authors.affiliation: NMI Natural and Medical Sciences Institute at the University of Tuebingen, Markwiesenstr. 55, 72770 Reutlingen, Germany.
authors.forename: Hannes
authors.initials: H
authors.lastname: Planatscher
authors.forename: Stefanie
authors.initials: S
authors.lastname: Rimmele
authors.forename: Gerd
authors.initials: G
authors.lastname: Michel
authors.forename: Oliver
authors.initials: O
authors.lastname: Pötz
authors.forename: Thomas
authors.initials: T
authors.lastname: Joos
authors.forename: Nicole
authors.initials: N
authors.lastname: Schneiderhan-Marra
mesh_terms: D000465:Algorithms; D000941:Antigens / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000592:standards; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D046228:Microarray Analysis / Q000592:standards; D008962:Models, Theoretical; D009169:Mycobacterium tuberculosis / Q000378:metabolism; D011786:Quality Control; D012016:Reference Values; D014376:Tuberculosis / Q000175:diagnosis
chemical_list: D000941:Antigens; D001426:Bacterial Proteins; D007074:Immunoglobulin G