title: Gene reporter assay to validate microRNA targets in Drosophila S2 cells.
abstract: Bioinformatics programs have helped tremendously in identifying the targets of microRNAs, which are small noncoding RNAs that regulate gene expression posttranscriptionally. However, the partial complementarity between miRNAs and their targets hinders the accuracy of target prediction, necessitating the use of experimental validation procedures. Here, we describe a gene reporter assay typically used in our lab to validate putative miRNA-mRNA interactions in Drosophila S2 cells.
authors.affiliation: Molecular Biology and Genetics, Izmir Institute of Technology, Izmir, Turkey.
authors.forename: Bünyamin
authors.initials: B
authors.lastname: Akgül
authors.forename: Cağdaş
authors.initials: C
authors.lastname: Göktaş
mesh_terms: D000818:Animals; D001483:Base Sequence; D004330:Drosophila / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs