title: Comparative proteomic and bioinformatic analysis of the effects of a high-grain diet on the hepatic metabolism in lactating dairy goats.
abstract: To gain insight on the impart of high-grain diets on liver metabolism in ruminants, we employed a comparative proteomic approach to investigate the proteome-wide effects of diet in lactating dairy goats by conducting a proteomic analysis of the liver extracts of 10 lactating goats fed either a control diet or a high-grain diet. More than 500 protein spots were detected per condition by two-dimensional electrophoresis (2-DE). In total, 52 differentially expressed spots (≥2.0-fold changed) were excised and analyzed using MALDI TOF/TOF. Fifty-one protein spots were successfully identified. Of these, 29 proteins were upregulated, while 22 were downregulated in the high-grain fed vs. control animals. Differential expressions of proteins including alpha enolase, elongation factor 2, calreticulin, cytochrome b5, apolipoprotein A-I, catalase, was verified by mRNA analysis and/or Western blotting. Database searches combined with Gene Ontology (GO) analysis and KEGG pathway analysis revealed that the high-grain diet resulted in altered expression of proteins related to amino acids metabolism. These results suggest new candidate proteins that may contribute to a better understanding of the signaling pathways and mechanisms that mediate liver adaptation to high-grain diet.
authors.affiliation: Key Laboratory of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, People's Repulic of China.
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Jiang
authors.forename: Tao
authors.initials: T
authors.lastname: Zeng
authors.forename: Shukun
authors.initials: S
authors.lastname: Zhang
authors.forename: Yuanshu
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D019295:Computational Biology; D004032:Diet; D005260:Female; D005786:Gene Expression Regulation; D006041:Goats / Q000235:genetics / Q000378:metabolism*; D007774:Lactation; D008099:Liver / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D058977:Molecular Sequence Annotation; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results