title: Kosmotoga pacifica sp. nov., a thermophilic chemoorganoheterotrophic bacterium isolated from an East Pacific hydrothermal sediment.
abstract: A novel strictly anaerobic thermophilic heterotrophic bacterium, strain SLHLJ1(T), was isolated from a Pacific hydrothermal sediment. Cells were Gram-negative coccobacilli (approximately 1.0 × 0.6 μm) with a toga. It grew at temperatures between 33 and 78 °C (optimum 70 °C). Elemental sulphur and L-cystine stimulated its growth. It contained C16:0, C16:1 ω11c, C18:0 and C18:1 ω9c as major fatty acids (>5%), 3 phospholipids and 2 glycolipids as polar lipids. Its DNA G+C content was 43.7 mol%. Phylogenetic analyses based on 16S rRNA gene sequences placed strain SLHLJ1(T) within the family Thermotogaceae. The novel isolate was most closely related to Kosmotoga arenicorallina (97.93 % 16S rRNA gene sequence similarity), K. olearia (92.43%) and K. shengliensis (92.17 %). On the basis of phenotypic, chemotaxonomic and phylogenetic comparisons with its closest relatives, we propose its assignment to a novel species of the genus Kosmotoga. The name Kosmotoga pacifica sp. nov. is proposed with strain SLHLJ1(T) (=DSM 26965(T) = JCM 19180(T) = UBOCC 3254(T)) as the type species.
authors.affiliation: Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), Université de Bretagne Occidentale (UBO, UEB), Institut Universitaire Européen de la Mer (IUEM), UMR 6197, Place Nicolas Copernic, 29280, Plouzané, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: L'Haridon
authors.forename: Lijing
authors.initials: L
authors.lastname: Jiang
authors.forename: Karine
authors.initials: K
authors.lastname: Alain
authors.forename: Morgane
authors.initials: M
authors.lastname: Chalopin
authors.forename: Ouafae
authors.initials: O
authors.lastname: Rouxel
authors.forename: Mickaël
authors.initials: M
authors.lastname: Beauverger
authors.forename: Hongxiu
authors.initials: H
authors.lastname: Xu
authors.forename: Zongze
authors.initials: Z
authors.lastname: Shao
authors.forename: Mohamed
authors.initials: M
authors.lastname: Jebbar
mesh_terms: D000693:Anaerobiosis; D019015:Geologic Sediments / Q000382:microbiology*; D006089:Gram-Negative Anaerobic Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D052836:Heterotrophic Processes; D006358:Hot Temperature; D060086:Hydrothermal Vents / Q000382:microbiology*; D010140:Pacific Ocean; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013455:Sulfur / Q000378:metabolism
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013455:Sulfur