title: Selective enrichment of phosphopeptides by titania nanoparticles coated magnetic carbon nanotubes.
abstract: Selective enrichment of phosphoproteins or phosphopeptides from complex mixtures is essential for mass spectrometry (MS)-based phosphoproteomics. In this work, for the first time, titania nanoparticles coated magnetic carbon nanotubes (denoted as MagCNTs@TiO₂ composites) were synthesized through a facile but effective solvothermal reaction for selective enrichment of phosphopeptides. The MagCNTs@TiO₂ material demonstrated low limit of detection (20 fmol), along with an exceptional great specificity to capture phosphopeptides from a tryptic digest of the mixture of a nonphosphorylated protein BSA and a phosphorylated protein β-casein with molar ratios of BSA/β-casein up to 200:1. In addition, the high magnetic susceptibility allowed convenient separation of the target peptides by magnetic separation. Experimental results demonstrated that the MagCNTs@TiO₂ composites showed excellent potential for the selective enrichment of phosphopeptides for MS analysis.
authors.affiliation: Department of Chemistry, Fudan University, Shanghai 200433, China; Institutes of Biomedical Science, Fudan University, Shanghai 200433, China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Yan
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Zheng
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Zhang
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Yang
mesh_terms: D000818:Animals; D002364:Caseins / Q000737:chemistry; D002417:Cattle; D006801:Humans; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008863:Microspheres; D037742:Nanotubes, Carbon; D010748:Phosphopeptides / Q000032:analysis* / Q000097:blood; D010766:Phosphorylation; D012710:Serum Albumin, Bovine / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014025:Titanium / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D002364:Caseins; D037742:Nanotubes, Carbon; D010748:Phosphopeptides; C009495:titanium dioxide; D012710:Serum Albumin, Bovine; D014025:Titanium
keywords: MagCNTs@TiO(2) composites; Mass spectrometry analysis; Phosphopeptides; Selective enrichment