title: Cytotoxic effects of acrylonitrile on human umbilical cord mesenchymal stem cells in vitro.
abstract: The effects of acrylonitrile (ACN) on human umbilical cord mesenchymal stem cells (hUC‑MSCs) remain unknown. The proliferation, differentiation, clonogenicity and apoptosis effects of ACN and/or N‑acetyl‑L‑cysteine (NAC) on hUC‑MSCs were investigated. The results showed that although ACN at a concentration of 0.1 µg/ml did not affect proliferation or the morphology of hUC‑MSCs compared with the control, osteogenic differentiation and the positive rate of alkaline phosphatase staining in the experimental group were significantly lower compared with the control (P<0.01). All of the effects of ACN were counteracted using NAC, a typical antioxidant. Using a flow cytometry assay, it was observed that ACN induced apoptosis in hUC‑MSCs. The results indicated that the toxic effect produced by ACN on hUC‑MSCs is based on a redox mechanism.
authors.affiliation: School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Sun
authors.forename: Min
authors.initials: M
authors.lastname: Sun
authors.forename: Yan
authors.initials: Y
authors.lastname: Xie
authors.forename: Wei
authors.initials: W
authors.lastname: Zhai
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.forename: Rui
authors.initials: R
authors.lastname: Ma
authors.forename: Rongzhu
authors.initials: R
authors.lastname: Lu
authors.forename: Wenrong
authors.initials: W
authors.lastname: Xu
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D000181:Acrylonitrile / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D000975:Antioxidants; D000181:Acrylonitrile; D000111:Acetylcysteine