title: βTrCP-mediated ubiquitylation regulates protein stability of Mis18β in a cell cycle-dependent manner.
abstract: Ubiquitin E3 ligases including SCF complex are key regulators of cell cycle. Here, we show that Mis18β, a component of Mis18 complex governing CENP-A localization, is a new substrate of βTrCP-containing SCF complex. βTrCP interacted with Mis18β exclusively during interphase but not during mitosis and mediated proteasomal degradation of Mis18β leading to the inactivation of Mis18 complex during interphase. In addition, uncontrolled stabilization of Mis18β caused cell death. Together, we propose that βTrCP-mediated regulation of Mis18β stability is a mechanism to restrict centromere function of Mis18 complex from late mitosis to early G1 phase.
authors.affiliation: Department of Biological Sciences, Creative Research Initiative Center for Chromatin Dynamics, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Ik Soo
authors.initials: IS
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Creative Research Initiative Center for Chromatin Dynamics, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Minkyoung
authors.initials: M
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, Sookmyung Women's University, Seoul 140-742, South Korea.
authors.forename: Joo Hyeon
authors.initials: JH
authors.lastname: Park
authors.affiliation: Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul 140-742, South Korea.
authors.forename: Raok
authors.initials: R
authors.lastname: Jeon
authors.affiliation: Department of Biological Sciences, Creative Research Initiative Center for Chromatin Dynamics, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Sung Hee
authors.initials: SH
authors.lastname: Baek
authors.affiliation: Department of Biological Sciences, Sookmyung Women's University, Seoul 140-742, South Korea. Electronic address: kikim@sookmyung.ac.kr.
authors.forename: Keun Il
authors.initials: KI
authors.lastname: Kim
mesh_terms: D020816:Amino Acid Motifs; D002453:Cell Cycle; D018797:Cell Cycle Proteins; D002503:Centromere / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D055550:Protein Stability; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D044843:SKP Cullin F-Box Protein Ligases / Q000378:metabolism*; D054875:Ubiquitination; D044844:beta-Transducin Repeat-Containing Proteins / Q000378:metabolism*
chemical_list: C471644:BTRC protein, human; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; C517146:OIP5 protein, human; D034741:RNA, Small Interfering; D044844:beta-Transducin Repeat-Containing Proteins; D044843:SKP Cullin F-Box Protein Ligases
keywords: CDK; CHX; Centromere; H3S10ph; Mis18β; Mitosis; Protein stability; SCF; Skp1/Cul1/F-box protein complex; Ubiquitylation; aa; amino acid; cyclin-dependent kinase; cycloheximide; phosphorylation at 10th serine residue of histone H3; βTrCP