title: Polymorphic Amplified Typing Sequences (PATS) Strain Typing System Accurately Discriminates a Set of Temporally and Spatially Disparate Escherichia coli O157 Isolates Associated with Human Infection.
abstract: Polymorphic Amplified Typing Sequences (PATS) is a PCR-based Escherichia coli O157 (O157) strain typing system. Here, we show that PATS compares excellently with Pulsed-Field Gel Electrophoresis (PFGE) in that both methods cluster geographically diverse O157 isolates similarly. Comparative analysis of the results obtained in this simulated "blind" study attests to the discriminating power and applicability of PATS to epidemiological/nosocomial situations.
authors.affiliation: Food Safety and Enteric Pathogens Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, IA. 50010.
authors.forename: Indira T
authors.initials: IT
authors.lastname: Kudva
authors.forename: Sandra
authors.initials: S
authors.lastname: Smole
authors.forename: Robert W
authors.initials: RW
authors.lastname: Griffin
authors.forename: Jeonifer
authors.initials: J
authors.lastname: Garren
authors.forename: Nimisha
authors.initials: N
authors.lastname: Kalia
authors.forename: Megan
authors.initials: M
authors.lastname: Murray
authors.forename: Manohar
authors.initials: M
authors.lastname: John
authors.forename: Ralph
authors.initials: R
authors.lastname: Timperi
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Calderwood
keywords: Bacteria; O157; PATS; PCR; PFGE.; Strain-typing