title: Isolation and characterization of a glucan-type polysaccharide from the red pine mushroom, Lactarius deliciosus (Higher Basidiomycetes).
abstract: A novel glucan-type polysaccharide has been isolated from the fruiting bodies of the edible mushroom Lactarius deliciosus. Two successive extractions (cold aqueous extraction at 25°C and hot aqueous extraction at 100°C) were performed, and the same polysaccharide was obtained in both fractions. The purity of the polysaccharide was evaluated by size exclusion chromatography. The SEC chromatogram showed a unique peak with a molecular weight of approximately 150 kDa. Analysis of the chain composition revealed that the polysaccharide was composed of glucose. Methylation analysis and NMR experiments showed that the glucan-type carbohydrate contained a main chain consisting of α-(1→3)-Glcp units with α-(1→4)-linked branches every sixth glucose residue.
authors.affiliation: Centro para la Calidad de los Alimentos, Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA), Campus Universitario Duques de Soria, c/ Jose Tudela s/n, 42004, Soria, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Villares
mesh_terms: D001487:Basidiomycota / Q000737:chemistry*; D001688:Biological Products / Q000737:chemistry*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D005936:Glucans / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D001688:Biological Products; D005936:Glucans; D011134:Polysaccharides