title: Colorimetric detection of hepatitis E virus based on reverse transcription loop mediated isothermal amplification (RT-LAMP) assay.
abstract: Hepatitis E virus (HEV) infection is a common communicable disease in the third world countries. The early diagnosis of HEV is important for the prognosis and prevention of the spread of the disease. Conventional methods for HEV detection need expensive equipment and cannot meet the needs of point of care (POC) use. In the present study, a gold nanoparticle (AuNP) based RT-LAMP assay was developed for colorimetric detection of HEV. As low as 10(1) copies of HEV RNA were detected visually. With reduced handling and less equipment requirement, this assay provided a simple and rapid alternative to other available methods.
authors.affiliation: The 171 hospital of People's Liberation Army, 332000 Jiang xi, China.
authors.forename: Qiuhua
authors.initials: Q
authors.lastname: Chen
authors.affiliation: People's Liberation Army, No 452 Hospital of Chengdu, 610021 Sichuan, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Chinese PLA 458th Hospital, 510602 Guangdong, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wan
authors.affiliation: The 171 hospital of People's Liberation Army, 332000 Jiang xi, China.
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Chen
authors.affiliation: The 171 hospital of People's Liberation Army, 332000 Jiang xi, China. Electronic address: Dcy1111@163.com.
authors.forename: Chaoyang
authors.initials: C
authors.lastname: Du
mesh_terms: D003124:Colorimetry / Q000379:methods*; D006046:Gold; D016751:Hepatitis E / Q000175:diagnosis* / Q000821:virology; D016752:Hepatitis E virus / Q000302:isolation & purification*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D053758:Nanoparticles; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D048348:Reverse Transcription
chemical_list: D006046:Gold
keywords: Colorimetric detection; Hepatitis E virus; Loop mediated isothermal amplification