title: [Effects of glyphosate on apoptosis and expressions of androgen-binding protein and vimentin mRNA in mouse Sertoli cells].
abstract: OBJECTIVE
  To investigate the effect of different doses of glyphosate on apoptosis and expressions of androgen-binding protein (ABP) and vimentin mRNA in mouse Sertoli cells.
  
  
  METHODS
  Primarily cultured mouse Sertoli cells incubated with different doses of glyphosate (60, 90, 120, 150 and 180 mg/L) for 24 h. The growth and morphological alterations in the cells were observed under inverted microscope, and the cell proliferation rate was evaluated withMTT assay. Hoechst 33342 staining was used to detect cell apoptosis after the treatment, and RT-PCR was performed to examine the changes in the expression of ABP and vimentin mRNAs.
  
  
  RESULTS
  Sertoli cells exposed to glyphosate showed a reduced cell volume, cell dissociation with occasional cell disruption. The proliferation of the exposed was suppressed with an increased rate of cell apoptosis and lowered expressions of ABP and vimentin mRNAs (P<0.05).
  
  
  CONCLUSIONS
  GLY can cause cellular damages, inhibit cell proliferation, induce cell apoptosis, and decrease expression of ABP and vimentin mRNAs in mouse Sertoli cells in vitro.
authors.affiliation: Department of Preventive Medicine, Bengbu Medical College, Bengbu 233030, China.E-mail: zwh3104@sina.com.
authors.forename: Wenhong
authors.initials: W
authors.lastname: Zhao
authors.forename: Hui
authors.initials: H
authors.lastname: Yu
authors.forename: Jianguo
authors.initials: J
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Shu
mesh_terms: D000727:Androgen-Binding Protein / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005998:Glycine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000633:toxicity; D006540:Herbicides / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism; D012708:Sertoli Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014746:Vimentin / Q000235:genetics* / Q000378:metabolism; D000097797:Glyphosate
chemical_list: D000727:Androgen-Binding Protein; D005998:Glycine; D006540:Herbicides; D012333:RNA, Messenger; D014746:Vimentin; D000097797:Glyphosate