title: Long term storage of callus cultures at low temperatures or under mineral oil layer.
abstract: Plant tissue cultures from various species were stored at low temperatures or under mineral oil overlay for 4 to 6 months without subcultures. After transfer to normal culture conditions, it was checked, with 3 strains, that growth characteristics and secondary metabolite production were preserved. The storage with a mineral oil overlay (easy to run and economical method) could be a possible alternative to cryogenic or low temperature storage for a large number of strains.
authors.affiliation: L.E.R. Synthelabo, BP 0166, F-37001, Tours Cedex, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Augereau
authors.forename: D
authors.initials: D
authors.lastname: Courtois
authors.forename: V
authors.initials: V
authors.lastname: Petiard