title: Rapid analysis of Fructus forsythiae essential oil by ionic liquids-assisted microwave distillation coupled with headspace single-drop microextraction followed by gas chromatography-mass spectrometry.
abstract: A rapid, green and effective miniaturized sample preparation and analytical technique, i.e. ionic liquids-assisted microwave distillation coupled with headspace single-drop microextraction (ILAMD-HS-SDME) followed by gas chromatography-mass spectrometry (GC-MS) was developed for the analysis of essential oil (EO) in Fructus forsythiae. In this work, ionic liquids (ILs) were not only used as the absorption medium of microwave irradiation but also as the destruction agent of plant cell walls. 1-Ethyl-3-methylimidazolium acetate ([C2mim]OAc) was chosen as the optimal ILs. Moreover, n-heptadecane (2.0 μL) was selected as the appropriate suspended solvent for the extraction and concentration of EO. Extraction conditions of the proposed method were optimized using the relative peak area of EO constituents as the index, and the optimal operational parameters were obtained as follows: irradiation power (300 W), sample mass (0.7 g), mass ratio of ILs to sample (2.4), temperature (78°C) and time (3.4 min). In comparison to previous reports, the proposed method was faster and required smaller sample amount but could equally monitor all EO constituents with no significant differences.
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, PR China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Jiao
authors.forename: Dan-Hui
authors.initials: DH
authors.lastname: Ma
authors.forename: Qing-Yan
authors.initials: QY
authors.lastname: Gai
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Meng
authors.initials: M
authors.lastname: Luo
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Fu
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D059627:Liquid Phase Microextraction; D008855:Microscopy, Electron, Scanning; D008872:Microwaves; D009822:Oils, Volatile / Q000032:analysis*
chemical_list: D004365:Drugs, Chinese Herbal; D009822:Oils, Volatile
keywords: Essential oil; Fructus forsythiae; Gas chromatography–mass spectrometry; Headspace single-drop microextraction; Ionic liquids; Miniaturized sample preparation technique