title: Novel chemically modified bacterial cellulose nanocomposite as potential biomaterial for stem cell therapy applications.
abstract: Bacterial cellulose (BC) has become established as a remarkably versatile biomaterial and can be used in a wide variety of applied scientific applications, especially for medical devices. In this work, the bacterial cellulose fermentation process is modified by the addition of hyaluronic acid and gelatin (1% w/w) to the culture medium before the bacteria is inoculated. Hyaluronic acid and gelatin influence in bacterial cellulose was analyzed using Transmission Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Adhesion and viability studies with human dental pulp stem cells using natural bacterial cellulose/hyaluronic acid as scaffolds for regenerative medicine are presented for the first time in this work. MTT viability assays show higher cell adhesion in bacterial cellulose/gelatin and bacterial cellulose/ hyaluronic acid scaffolds over time with differences due to fiber agglomeration in bacterial cellulose/gelatin. Confocal microscopy images showed that the cell were adhered and well distributed within the fibers in both types of scaffolds.
authors.forename: Gerson Arisoly
authors.initials: GA
authors.lastname: Xavier Acasigua
authors.forename: Gabriel Molina
authors.initials: GM
authors.lastname: de Olyveira
authors.forename: Ligia Maria
authors.initials: LM
authors.lastname: Manzine Costa
authors.forename: Daikelly Iglesias
authors.initials: DI
authors.lastname: Braghirolli
authors.forename: Anna Christina
authors.initials: AC
authors.lastname: Medeiros Fossati
authors.forename: Antonio Carlos
authors.initials: AC
authors.lastname: Guastaldi
authors.forename: Patricia
authors.initials: P
authors.lastname: Pranke
authors.forename: Gildásio de Cerqueira
authors.initials: Gde C
authors.lastname: Daltro
authors.affiliation: Department of Physical Chemistry- UNESP/Araraquara-SP, 14800-900, Brazil. gmolyveira@yahoo.com.br.
authors.forename: Pierre
authors.initials: P
authors.lastname: Basmaji
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D064987:Cell- and Tissue-Based Therapy; D002478:Cells, Cultured; D002482:Cellulose / Q000737:chemistry* / Q000648:ultrastructure; D005780:Gelatin / Q000737:chemistry / Q000648:ultrastructure; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D008567:Membranes, Artificial; D059630:Mesenchymal Stem Cells / Q000502:physiology; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D016062:Porosity; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011135:Polysaccharides, Bacterial; D005780:Gelatin; D002482:Cellulose; D006820:Hyaluronic Acid