title: HER-2/neu targeted delivery of a nanoprobe enables dual photoacoustic and fluorescence tomography of ovarian cancer.
abstract: Development of sensitive and specific imaging approaches for the detection of ovarian cancer holds great promise for improving survival of ovarian cancer patients. Here we describe a dual-modality photoacoustic and fluorescence molecular tomography (PAT/FMT) approach in combination with a targeted imaging probe for three-dimensional imaging of ovarian tumors in mice. We found that the selective accumulation of the HER-2/neu targeted magnetic iron oxide nanoparticles (IONPs) led to about 5-fold contrast enhancements in the tumor for PAT, while near-infrared (NIR) dye labeled nanoparticles emitted strong optical signals for FMT. Both PAT and FMT were demonstrated to be able to detect ovarian tumors located deep in the peritoneal cavity in mice. The targeted nanoprobes allowed mapping tumors in high resolution via PAT, and high sensitivity and specificity via FMT. This study demonstrated the potential of the application of HER-2/neu-targeted PAT/FMT approach for non-invasive or intraoperative imaging of ovarian cancer.
  
  
  BACKGROUND
  This paper details the development of a dual-modality photoacoustic and fluorescence molecular tomography approach in combination with a targeted imaging probe for three-dimensional imaging of ovarian tumors in a mouse model, demonstrating the application of the HER-2/neu-targeted approach for non-invasive or intraoperative imaging of ovarian cancer.
authors.affiliation: Department of Biomedical Engineering, University of Florida, Gainesville, FL.
authors.forename: Lei
authors.initials: L
authors.lastname: Xi
authors.affiliation: Department of Surgery, Emory University School of Medicine, Atlanta, GA.
authors.forename: Minati
authors.initials: M
authors.lastname: Satpathy
authors.affiliation: Department of Biomedical Engineering, University of Florida, Gainesville, FL.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Surgery, Emory University School of Medicine, Atlanta, GA.
authors.forename: Weiping
authors.initials: W
authors.lastname: Qian
authors.affiliation: Department of Surgery, Emory University School of Medicine, Atlanta, GA. Electronic address: lyang02@emory.edu.
authors.forename: Lily
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Biomedical Engineering, University of Florida, Gainesville, FL. Electronic address: hjiang@bme.ufl.edu.
authors.forename: Huabei
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D005290:Ferric Compounds; D005456:Fluorescent Dyes; D006801:Humans; D051379:Mice; D057054:Molecular Imaging / Q000295:instrumentation; D053758:Nanoparticles; D061848:Optical Imaging / Q000295:instrumentation*; D010051:Ovarian Neoplasms / Q000473:pathology*; D010053:Ovary / Q000473:pathology*; D061088:Photoacoustic Techniques / Q000295:instrumentation*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D014054:Tomography / Q000295:instrumentation
chemical_list: D005290:Ferric Compounds; D005456:Fluorescent Dyes; D018719:Erb-b2 Receptor Tyrosine Kinases; C000499:ferric oxide
keywords: Fluorescence molecular tomography; HER-2/neu targeted nanoparticles; Iron oxide nanoparticle; Photoacoustic tomography