title: Biochemical and antioxidant properties of peptidic fraction of carotenoproteins generated from shrimp by-products by enzymatic hydrolysis.
abstract: The composition, functional properties and in vitro antioxidative activity of the peptidic fraction of carotenoproteins from shrimp (Parapenaeus longirostris) by-products generated by enzymatic treatment with Alcalase was evaluated. The peptidic fraction of carotenoproteins (PFCP) contained 80.8 ± 0.21% protein, 2.74 ± 0.3% lipid, 14.4 ± 0.14% ash, 1.13 ± 0.08% chitin and 1.08 ± 0.02 μg total carotenoid/g of sample. The amino acid profile of PFCP showed a high percentage of essential amino acids, such as arginine, lysine, histidine and leucine. Therefore, PFCP had a high nutritional value and could be used as a supplement to poorly balanced dietary proteins. PFCP showed an excellent solubility and possessed interfacial properties, which were governed by their concentrations. The antioxidant activities of PFCP at different concentrations were evaluated using various in vitro antioxidant assays, including the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical method, reducing power, chelating effects assay and β-carotene bleaching. The antioxidant activity of PFCP, based on their protection of supercoiled DNA strand from scission by peroxyl and hydroxyl radicals into the nicked circular form was also investigated. Results from this study suggest that the peptidic fraction of carotenoproteins is a good source of natural antioxidants and peptides with interesting functionalities.
authors.affiliation: Unité Enzymes et Bioconversion, National School of Engineering, PB 1173, 3038 Sfax, Tunisia; Laboratoire de Procédés Biologiques, Génie Enzymatique et Microbien, IUT A Lille I, BP 179, 59653 Villeneuve d'Ascq Cedex, France.
authors.forename: Assaâd
authors.initials: A
authors.lastname: Sila
authors.forename: Nadhem
authors.initials: N
authors.lastname: Sayari
authors.forename: Rafik
authors.initials: R
authors.lastname: Balti
authors.forename: Oscar
authors.initials: O
authors.lastname: Martinez-Alvarez
authors.forename: Naima
authors.initials: N
authors.lastname: Nedjar-Arroume
authors.forename: Nasri
authors.initials: N
authors.lastname: Moncef
authors.forename: Ali
authors.initials: A
authors.lastname: Bougatef
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000975:Antioxidants / Q000737:chemistry*; D006868:Hydrolysis; D033561:Penaeidae / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013381:Subtilisins / Q000737:chemistry*; D014866:Waste Products / Q000032:analysis*
chemical_list: D000596:Amino Acids; D000975:Antioxidants; D010455:Peptides; D011506:Proteins; D014866:Waste Products; D013381:Subtilisins
keywords: Antioxidative activity; Carotenoproteins; Functional properties; Parapenaeus longirostris; Peptidic fraction