title: Genetic control of endosperm proteins in wheat : 3. Allocation to chromosomes and differential expression of high molecular weight glutenin and gliadin genes in intervarietal substitution lines of common wheat.
abstract: Total endosperm proteins extracted from both several common wheat cultivars and some intervarietal substitution lines derived from them were fractionated according to their molecular weight in a high resolution one-dimensional gel electrophoresis. The four donor cultivars and the recipient one - 'Chinese Spring', possessed differentially migrating protein bands in the fractions of high molecular weight (HMW) glutenins and gliadins. Several of these bands were identified for the first time in this study. By utilizing intervarietal substitution lines the control of the HMW glutenins and gliadins by chromosomes of homoeologous group 1 was either reaffirmed or, for the new bands, established. Several HMW gliadin subunits showed a considerable variation in their staining intensity in the intervarietal substitution lines indicating that their expression was dependent on the genetic background.
authors.affiliation: Plant Genetics, The Weizmann Institute of Science, 76 100, Rehovot, Israel.
authors.forename: G
authors.initials: G
authors.lastname: Galili
authors.forename: M
authors.initials: M
authors.lastname: Feldman