title: Expression of Anabaena ferredoxin genes in Escherichia coli.
abstract: The genes for ferredoxin from heterocysts (fdx H) and vegetative cells (pet F) of Anabaena sp. strain 7120 were subcloned into plasmid pUC 18/19. Both genes were expressed in Escherichia coli at high levels (≈10% of total protein). Pet F could be expressed from its own promoter. The ferredoxins were correctly assembled to the holoprotein. Heterocyst ferredoxin was purified from E. coli extracts on a large scale. Its biochemical and biophysical properties were identical to those of the authentic ferredoxin, isolated from Anabaena heterocysts.
authors.affiliation: Department of Molecular Genetics and Cell Biology, University of Chicago, 60637, Chicago, IL, USA.
authors.forename: H
authors.initials: H
authors.lastname: Böhme
authors.forename: R
authors.initials: R
authors.lastname: Haselkorn