title: Zingerone regulates intestinal transit, attenuates behavioral and oxidative perturbations in irritable bowel disorder in rats.
abstract: Stress can lead to the manifestation of functional gastrointestinal disorders, the most prominent being irritable bowel disorder. The present study investigated the impact zingerone in ameliorating chronic water stress induced irritable bowel disorder, brain gut axis dysfunction and dysregulation of the intestinal barrier due to oxidative stress. Rats were randomly allocated to groups and subjected to chronic water stress for a period of 21 days for 1h and the fecal pellet output was measured. At the end of chronic stress, behavioral assessment for anxiety like behavior was recorded and plasma corticosterone levels were measured 60min after water stress. The colonic transit was determined, levels of oxidative and antioxidant biomarkers were measured in the colon homogenate. Myeloperoxidase activity was determined as an indirect index of neutrophil infiltration. Chronic water stress increased the rate of colonic transit, fecal output, induced behavioral changes, and decreased antioxidant levels. An increase in lipid peroxide levels, catalase and corticosterone was observed. Mast cell infiltration was evident in the stressed group. Zingerone significantly reduced colonic transit, fecal output, neutrophil infiltration, and lipid peroxide formation. The levels of catalase were not altered; however, a marginal increase in the levels of glutathione peroxidase was observed. Zingerone significantly enhanced the levels of superoxide dismutase, glutathione and decreased the levels of corticosterone. Zingerone produced marked improvement in stress induced irritable bowel disorder which could be attributed to the powerful antioxidant nature, direct effect on the intestinal smooth muscle and adaptogenic nature.
authors.affiliation: Nalanda College of Pharmacy, Hyderabad Road, Nalgonda, Andhra Pradesh, India. Electronic address: davidbanji@gmail.com.
authors.forename: David
authors.initials: D
authors.lastname: Banji
authors.affiliation: Nalanda College of Pharmacy, Hyderabad Road, Nalgonda, Andhra Pradesh, India.
authors.forename: Otilia J F
authors.initials: OJ
authors.lastname: Banji
authors.affiliation: Nalanda College of Pharmacy, Hyderabad Road, Nalgonda, Andhra Pradesh, India.
authors.forename: Bandlapalli
authors.initials: B
authors.lastname: Pavani
authors.affiliation: Rajah Muthaih Medical College, Annamalai University, Annamalainagar, Tamilnadu, India.
authors.forename: Ch
authors.initials: Ch
authors.lastname: Kranthi Kumar
authors.affiliation: Rajah Muthaih Medical College, Annamalai University, Annamalainagar, Tamilnadu, India.
authors.forename: A R
authors.initials: AR
authors.lastname: Annamalai
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003106:Colon / Q000187:drug effects; D003345:Corticosterone / Q000097:blood; D004353:Drug Evaluation, Preclinical; D005772:Gastrointestinal Transit / Q000187:drug effects*; D020939:Zingiber officinale; D006139:Guaiacol / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D043183:Irritable Bowel Syndrome / Q000188:drug therapy* / Q000209:etiology; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D011897:Random Allocation; D017208:Rats, Wistar
chemical_list: D010936:Plant Extracts; C013738:zingerone; D006139:Guaiacol; D003345:Corticosterone
keywords: Colonic transit; Mast cells; Myeloperoxidase; Zingerone; Zingiber officinalis