title: Antibacterial electrospun nanofibers from triclosan/cyclodextrin inclusion complexes.
abstract: The electrospinning of nanofibers (NF) from cyclodextrin inclusion complexes (CD-IC) with an antibacterial agent (triclosan) was achieved without using any carrier polymeric matrix. Polymer-free triclosan/CD-IC NF were electrospun from highly concentrated (160% CD, w/w) aqueous triclosan/CD-IC suspension by using two types of chemically modified CD; hydroxypropyl-beta-cyclodextrin (HPβCD) and hydroxypropyl-gamma-cyclodextrin (HPγCD). The morphological characterization of the electrospun triclosan/CD-IC NF by SEM elucidated that the triclosan/HPβCD-IC NF and triclosan/HPγCD-IC NF were bead-free having average fiber diameter of 520 ± 250 nm and 1,100 ± 660 nm, respectively. The presence of triclosan and the formation of triclosan/CD-IC within the fiber structure were confirmed by (1)H-NMR, FTIR, XRD, DSC, and TGA studies. The initial 1:1 molar ratio of the triclosan:CD was kept for triclosan/HPβCD-IC NF after the electrospinning and whereas 0.7:1 molar ratio was observed for triclosan/HPγCD-IC NF and some uncomplexed triclosan was detected suggesting that the complexation efficiency of triclosan with HPγCD was lower than that of HPβCD. The antibacterial properties of triclosan/CD-IC NF were tested against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. It was observed that triclosan/HPβCD-IC NF and triclosan/HPγCD-IC NF showed better antibacterial activity against both bacteria compared to uncomplexed pure triclosan.
authors.affiliation: Institute of Materials Science & Nanotechnology and UNAM-National Nanotechnology Research Center, Bilkent University, Ankara 06800, Turkey.
authors.forename: Asli
authors.initials: A
authors.lastname: Celebioglu
authors.affiliation: Institute of Materials Science & Nanotechnology and UNAM-National Nanotechnology Research Center, Bilkent University, Ankara 06800, Turkey.
authors.forename: Ozgun C O
authors.initials: OC
authors.lastname: Umu
authors.affiliation: Institute of Materials Science & Nanotechnology and UNAM-National Nanotechnology Research Center, Bilkent University, Ankara 06800, Turkey; Gazi University, Life Sciences Application and Research Center, Ankara 06830, Turkey; Gazi University, Polatlı Science and Literature Faculty, Ankara 06900, Turkey.
authors.forename: Turgay
authors.initials: T
authors.lastname: Tekinay
authors.affiliation: Institute of Materials Science & Nanotechnology and UNAM-National Nanotechnology Research Center, Bilkent University, Ankara 06800, Turkey. Electronic address: tamer@unam.bilkent.edu.tr.
authors.forename: Tamer
authors.initials: T
authors.lastname: Uyar
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D003505:Cyclodextrins / Q000737:chemistry / Q000494:pharmacology*; D004553:Electric Conductivity; D004566:Electrodes; D004926:Escherichia coli / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D057139:Nanofibers / Q000737:chemistry*; D010316:Particle Size; D013211:Staphylococcus aureus / Q000187:drug effects*; D013499:Surface Properties; D014260:Triclosan / Q000737:chemistry / Q000494:pharmacology*; D014783:Viscosity
chemical_list: D000900:Anti-Bacterial Agents; D003505:Cyclodextrins; D014260:Triclosan
keywords: Antibacterial activity; Cyclodextrin; Electrospinning; Inclusion complex; Nanofibers; Triclosan