title: Modifying cellular properties using artificial aptamer-lipid receptors.
abstract: We demonstrate that artificial aptamer-lipid receptors (AR), which anchor on the surface of cells, can modify important cellular functions, including protein binding, enzymatic activity, and intercellular interactions. Streptavidin (SA)-AR-modified CEM cells captured the tetravalent SA with one biotin binding site. The remaining biotin sites captured biotinylated TDO5 aptamers, which target IgM on Ramos cells, to form CEM-Ramos cell assemblies. In another design, thrombin, an enzyme involved in blood clotting, was captured by thrombin-AR-modified cells and clot formation was visualized. Lastly, hematopoietic stem cell (HSC) mimics were modified with a tenascin-C-AR to improve the homing of HSC after an autologous bone marrow transplant. Tenascin-C-AR modified cells aggregated to cells in a tenascin-C expressing stem cell niche model better than library-AR modified cells. Modification of cellular properties using ARs is a one-step, dosable, nontoxic, and reversible method, which can be applied to any cell-type with any protein that has a known aptamer.
authors.affiliation: 1] Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Shands Cancer Center and UF Genetics Institute, University of Florida, Gainesville, Florida, 32611-7200, United States [2].
authors.forename: Meghan O
authors.initials: MO
authors.lastname: Altman
authors.forename: Yun Min
authors.initials: YM
authors.lastname: Chang
authors.forename: Xiangling
authors.initials: X
authors.lastname: Xiong
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
mesh_terms: D052157:Aptamers, Nucleotide / Q000378:metabolism*; D052158:Aptamers, Peptide / Q000378:metabolism*; D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin / Q000378:metabolism; D002449:Cell Aggregation / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006367:HeLa Cells; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D011485:Protein Binding; D062165:Receptors, Artificial / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D019809:Streptavidin / Q000737:chemistry; D019063:Tenascin / Q000737:chemistry / Q000378:metabolism; D013917:Thrombin / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D052158:Aptamers, Peptide; D007075:Immunoglobulin M; D062165:Receptors, Artificial; D011956:Receptors, Cell Surface; D019063:Tenascin; C081676:thrombin aptamer; D001710:Biotin; D019809:Streptavidin; D013917:Thrombin