title: Catabolism of 3-indole acetic acid in protoplasts from etiolated seedlings of scots pine (Pinus sylvestris L.).
abstract: Protoplasts preparated from dark grown seedlings of Pinus sylvestris L. were incubated with 3-indole (1-(14)C) acetic acid and 3-indole (2-(14)C) acetic acid (IAA). Three catabolites were consistently produced in the (2-(14)C) IAA feeds, one of which co-chromatographed with 3-indole methanol on reversed phase high performance liquid chromatography (HPLC). Protoplasts feed with (1-(14)C) IAA produced only one labelled catabolite. The non-decarboxylated compound formed was highly polar on reversed phase HPLC, both in the ion suppression and the ion pair mode. The substance was not hydrolysable at pH 11 and 100° indicating that it is not a conjugated form. Effects of time of incubation, pH and the cofactors hydrogen peroxide and 2,4-dichlorphenol on the catabolic rate of IAA are discussed.
authors.affiliation: Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-901 83, Umeå, Sweden.
authors.forename: G
authors.initials: G
authors.lastname: Sandberg
authors.forename: J E
authors.initials: JE
authors.lastname: Hällgren