title: Effects of transforming growth factor beta 1 on the plasminogen activation system, collagen and integrin synthesis, and proliferation of rabbit mandibular condylar chondrocytes.
abstract: The objective of this study was to identify the mechanism by which mandibular condyle chondrocytes regulate the extracellular matrix. Primary rabbit condylar chondrocytes were isolated, cultured, and treated with transforming growth factor beta 1 (TGF-β1). Cells were then assayed for the following: urokinase-type plasminogen activator (uPA) and its inhibitor (PAI-1), collagen types I and II, β1 integrin expression, and proliferative activity. TGF-β1 induced synthesis of collagen type II, αVβ1 integrin, and PAI-1. TGF-β1 induced the growth of chondrocytes and suppressed the synthesis of uPA. Chondrocyte regulation of the extracellular matrix is mediated by TGF-β1. Synthesis of collagen type II, αVβ1 integrin, and PAI-1 is induced, while uPA is suppressed. Also, TGF-β1 induces cellular growth.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Shimane University Faculty of Medicine, Shimane, Japan. Electronic address: bashi@med.shimane-u.ac.jp.
authors.forename: H
authors.initials: H
authors.lastname: Ishibashi
authors.affiliation: Department of Oral and Maxillofacial Surgery, Shimane University Faculty of Medicine, Shimane, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nariai
authors.affiliation: Department of Oral and Maxillofacial Surgery, Shimane University Faculty of Medicine, Shimane, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kanno
authors.affiliation: Department of Pathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Onimaru
authors.affiliation: Department of Oral and Maxillofacial Surgery, Shimane University Faculty of Medicine, Shimane, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Sekine
mesh_terms: D000328:Adult; D000369:Aged, 80 and over; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects*; D003094:Collagen / Q000096:biosynthesis*; D005109:Extracellular Matrix / Q000187:drug effects*; D006801:Humans; D007124:Immunoenzyme Techniques; D016023:Integrins / Q000096:biosynthesis*; D008335:Mandibular Condyle / Q000166:cytology; D017395:Plasminogen Activator Inhibitor 1 / Q000096:biosynthesis*; D011817:Rabbits; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis
chemical_list: D016023:Integrins; D017395:Plasminogen Activator Inhibitor 1; D053773:Transforming Growth Factor beta1; D003094:Collagen; D014568:Urokinase-Type Plasminogen Activator
keywords: TGF-β1; extracellular matrix; mandibular condyle