title: Transformation of sugarcane protoplasts by direct uptake of a selectable chimaeric gene.
abstract: Sugarcane protoplasts were transformed to kanamycin resistance at a frequency of approximately 8 in 10(7) following PEG-induced uptake of Sma1 linearised pABD1 plasmid. DNA-treated protoplasts were cultured in agarose droplets, and protoplast-derived transformants selected on 80 μg ml(-1) kanamycin. Transformed tissues maintained on this level of antibiotic expressed APH(3')II activity, and contained DNA that hybridised to a probe with the APH(3')II gene.
authors.affiliation: Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, University Park, Nottingham, UK.
authors.forename: W H
authors.initials: WH
authors.lastname: Chen
authors.forename: K M
authors.initials: KM
authors.lastname: Gartland
authors.forename: M R
authors.initials: MR
authors.lastname: Davey
authors.forename: R
authors.initials: R
authors.lastname: Sotak
authors.forename: J S
authors.initials: JS
authors.lastname: Gartland
authors.forename: B J
authors.initials: BJ
authors.lastname: Mulligan
authors.forename: J B
authors.initials: JB
authors.lastname: Power
authors.forename: E C
authors.initials: EC
authors.lastname: Cocking