title: Proofreading of noncognate acyl adenylates by an acyl-coenzyme a ligase.
abstract: Aminoacyl-tRNA synthetases remove (proofread) incorrect substrates and thereby prevent errors in protein synthesis. We report enzyme-catalyzed pretransfer editing by pimeloyl-coenzyme A (CoA) ligase (BioW), a biotin synthetic enzyme that converts pimelate, a seven-carbon dicarboxylic acid, to its CoA ester. The noncognate BioW substrate glutaric acid results in hydrolysis of ATP to AMP with formation of only trace amounts of glutaryl-CoA, thereby mimicking pretransfer editing of incorrect aminoacyl-adenylates by aminoacyl-tRNA synthetases.
authors.affiliation: Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
authors.forename: Miglena
authors.initials: M
authors.lastname: Manandhar
authors.affiliation: Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Electronic address: j-cronan@life.uiuc.edu.
authors.forename: John E
authors.initials: JE
authors.lastname: Cronan
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism*; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology* / Q000378:metabolism; D001710:Biotin / Q000031:analogs & derivatives; D003066:Coenzyme A Ligases / Q000378:metabolism*; D005977:Glutarates / Q000378:metabolism; D006868:Hydrolysis; D013379:Substrate Specificity
chemical_list: D000214:Acyl Coenzyme A; D005977:Glutarates; C019527:pimeloyl-coenzyme A; C015901:glutaryl-coenzyme A; D000249:Adenosine Monophosphate; D001710:Biotin; D000255:Adenosine Triphosphate; D000604:Amino Acyl-tRNA Synthetases; D003066:Coenzyme A Ligases; C035736:glutaric acid