title: Prevention of postsurgical cauterization-induced peritoneal adhesions by biodegradable and thermosensitive micelles.
abstract: Postsurgical peritoneal adhesion is a major concern in clinical practice which causes significant morbidity and mortality. In this study, we investigated the efficacy of biodegradable and injectable thermosensitive poly(ethylene glycol)-poly(epsilon-caprolactone)-poly(ethylene glycol) (PEG-PCL-PEG) micelles in preventing postsurgical cauterization-induced peritoneal adhesion. The biodegradable PEG-PCL-PEG copolymer could form nano-sized micelles in water, which instantly turned into a non-flowing gel at body temperature due to micellar aggregation. Moreover, a novel sidewall and cecum cauterization rat model was developed and the micelles were assigned for adhesion prevention tests. The PEG-PCL-PEG micelles could be administered by an ordinary syringe and provided unrestricted coverage of the cauterized peritoneum. The micelles instantly formed a gel in situ at body temperature and the formed gel could adhere to the cauterized sites as a durable barrier during critical time of adhesion formation. All rats from the control group (n = 10) developed score 5 adhesion, whereas, eight out of ten rats in the micelle-treated group showed no adhesion at all. Besides, cauterization-induced adhesion formation, adhesiveness and degradation of micelles, remesothelization of peritoneum, and restoration of cauterized tissue were investigated in detail. Our results thus indicated that, it was feasible to use biodegradable and injectable thermosensitive PEG-PCL-PEG micelles for prevention of peritoneal adhesions after surgery.
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Chang Yang
authors.initials: CY
authors.lastname: Gong
authors.forename: Qin Jie
authors.initials: QJ
authors.lastname: Wu
authors.forename: Jin Feng
authors.initials: JF
authors.lastname: Liao
authors.forename: Ting Ting
authors.initials: TT
authors.lastname: Qi
authors.forename: Bing
authors.initials: B
authors.lastname: Yang
authors.forename: Yu Jun
authors.initials: YJ
authors.lastname: Wang
authors.forename: Gang
authors.initials: G
authors.lastname: Guo
authors.forename: Feng
authors.initials: F
authors.lastname: Luo
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.forename: Yu Quan
authors.initials: YQ
authors.lastname: Wei
authors.forename: Zhi Yong
authors.initials: ZY
authors.lastname: Qian
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D002425:Cautery / Q000009:adverse effects* / Q000379:methods; D005260:Female; D008422:Materials Testing; D051379:Mice; D008823:Micelles; D041681:NIH 3T3 Cells; D010532:Peritoneal Diseases / Q000517:prevention & control*; D010537:Peritoneum / Q000187:drug effects / Q000601:surgery; D011091:Polyesters / Q000493:pharmacokinetics / Q000494:pharmacology / Q000627:therapeutic use*; D011092:Polyethylene Glycols / Q000493:pharmacokinetics / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013696:Temperature; D000267:Tissue Adhesions / Q000517:prevention & control*; D016896:Treatment Outcome
chemical_list: D008823:Micelles; D011091:Polyesters; C502908:poly(ethylene glycol)-poly(caprolactone)-poly(ethylene glycol); D011092:Polyethylene Glycols