title: Synthesis of petal-like ferric oxide/cysteine architectures and their application in affinity separation of proteins.
abstract: Petal-like ferric oxide/cysteine (FeOOH/Cys) architectures were prepared through a solvothermal route, which possessed high thiol group density. These thiol groups as binding sites can chelate Ni(2+) ions, which can be further used to enrich and separate his-tagged proteins directly from the mixture of lysed cells without sample pretreatment. These results show that the FeOOH/Cys architectures with immobilized Ni(2+) ions present negligible nonspecific protein adsorption and high protein adsorption capacity, with the saturation capacity being 88mg/g, which are especially suitable for purification of his-tagged proteins.
authors.affiliation: Key Laboratory for Special Functional Materials, Henan University, Kaifeng 475004, PR China.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Zou
authors.forename: Kun
authors.initials: K
authors.lastname: Li
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Yin
authors.forename: Yanbao
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Binjie
authors.initials: B
authors.lastname: Li
authors.forename: Shasha
authors.initials: S
authors.lastname: Yao
authors.forename: Chunpeng
authors.initials: C
authors.lastname: Song
mesh_terms: D029681:Arabidopsis Proteins / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D003545:Cysteine / Q000138:chemical synthesis* / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005290:Ferric Compounds / Q000138:chemical synthesis* / Q000737:chemistry*; D006639:Histidine; D008855:Microscopy, Electron, Scanning; D009842:Oligopeptides; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D029681:Arabidopsis Proteins; D005290:Ferric Compounds; C471213:His-His-His-His-His-His; D009842:Oligopeptides; D013438:Sulfhydryl Compounds; C000499:ferric oxide; D006639:Histidine; D003545:Cysteine
keywords: Ferric oxide/cysteine; His-tagged protein; Preparation; Separation