title: 14-3-3 gene expression exerts isoform-dependent functions in sinonasal pathophysiology.
abstract: The expression profiles of 14-3-3β and θ isoforms, known to exert both oncogenic and antiapoptotic effects, were assessed in different entities of nasal pathophysiology. Flow cytometry and immunohistochemistry were used on paraffin-embedded sections of 51 inverted papillomas (IP), 26 nasal polyps (NP), 9 polyps with IP (NPIP) and 10 specimens of normal epithelium (NE). 14-3-3β expression was significantly upregulated in IP as compared with both NP (p=0.015) and NE (p=0.002). 14-3-3β was also increased in NPIP as compared with NE (p=0.008). 14-3-3β cytoplasmic staining was more pronounced in basal cells of the respiratory epithelium although serous glands and the vascular system were often positive as well. High 14-3-3β immunopositivity in IP patients concurred with increased proliferative activity shown by PCNA immunostaining (p=0.04). Expression of 14-3-3θ was also found increased in IP and NPIP patients, compared to NP (p=0.005, p=0.002 respectively) and NE (p=0.004 and p=0.001 respectively). 14-3-3θ cytoplasmic immunopositivity was detected in columnar epithelium, particularly in basal and subluminal cells, whereas no immunoreactivity was observed in NP and NE. Our results demonstrate differential expression of 14-3-3β and θ isoforms in sinonasal pathophysiology, supporting their implication, respectively, in the proliferative and inflammatory process engaged in the formation of IP.
authors.affiliation: Laboratory of Surgical Research, 1st Department of Propaedeutic Surgery, University of Athens Hippocration Hospital, 114 Queen's Sofia Avenue, 11527 Greece.
authors.forename: Agapi
authors.initials: A
authors.lastname: Kataki
authors.affiliation: Laboratory of Surgical Research, 1st Department of Propaedeutic Surgery, University of Athens Hippocration Hospital, 114 Queen's Sofia Avenue, 11527 Greece. Electronic address: leonalevizos@gmail.com.
authors.forename: Leonidas
authors.initials: L
authors.lastname: Alevizos
authors.affiliation: Department of Pathology, Medical School, University of Athens, 75 Mikras Asias Street, Goudi, 11527 Athens, Greece.
authors.forename: Andreas
authors.initials: A
authors.lastname: Lazaris
authors.affiliation: Histopathological Diagnosis, Soutsou 5, 11527, Athens, Greece.
authors.forename: Georgia
authors.initials: G
authors.lastname: Glustianou
authors.affiliation: Laboratory of Surgical Research, 1st Department of Propaedeutic Surgery, University of Athens Hippocration Hospital, 114 Queen's Sofia Avenue, 11527 Greece.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Derventzi
authors.affiliation: 1st Department of Otolaryngology, University of Athens, Hippocration Hospital of Athens, 114 Queen's Sofia Avenue, 11527, Greece.
authors.forename: Evagelos
authors.initials: E
authors.lastname: Giotakis
authors.affiliation: Laboratory of Surgical Research, 1st Department of Propaedeutic Surgery, University of Athens Hippocration Hospital, 114 Queen's Sofia Avenue, 11527 Greece.
authors.forename: Manousos
authors.initials: M
authors.lastname: Konstadoulakis
authors.affiliation: 1st Department of Otolaryngology, University of Athens, Hippocration Hospital of Athens, 114 Queen's Sofia Avenue, 11527, Greece.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Yiotakis
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D049109:Cell Proliferation; D015331:Cohort Studies; D004847:Epithelial Cells; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism; D009298:Nasal Polyps / Q000378:metabolism* / Q000503:physiopathology; D009669:Nose Neoplasms / Q000378:metabolism* / Q000503:physiopathology; D010212:Papilloma / Q000378:metabolism* / Q000503:physiopathology; D018308:Papilloma, Inverted / Q000378:metabolism* / Q000503:physiopathology; D020033:Protein Isoforms; D055815:Young Adult
chemical_list: D048948:14-3-3 Proteins; D020033:Protein Isoforms; C482982:YWHAB protein, human
keywords: 14-3-3 isoforms; Inflammation; Inverted papilloma; Nasal polyps; Proliferation