title: Plantlets from somatic callus tissue of the East Indian Rosewood (Dalbergia latifolia Roxb.).
abstract: Callus-mediated shoot bud formation was demonstrated in Dalbergia latifolia Roxb. (East Indian Rosewood). Cultures were raised from shoot explants of six year-old plants on Murashige and Skoog (MS) medium supplemented with naphthaleneacetic acid (NAA) and benzyladenine (BA). A sequential treatment of callus with increasing BA levels and decreasing NAA ensured shoot bud induction. Rooting of shoots was achieved by a three-step culture procedure involving 1) White's(W) liquid medium containing indoleacetic acid (IAA), naphthaleneacetic acid and indolebutyric acid (IBA), 2) half-strength MS agar-solidified medium with charcoal (0.25%) and 3) half-strength MS liquid medium.
authors.affiliation: Enzymology and Plant Biochemistry Laboratory, Department of Biochemistry, Indian Institute of Science, 560 012, Bangalore, India.
authors.forename: K
authors.initials: K
authors.lastname: Sankara Rao