title: Haploid plants from in vitro anther culture of Annona squamosa Linn.
abstract: Haploid plants were induced from anther callus of Annona squamosa Linn. (Custard apple) on a Nitsch basal medium supplemented with 6-benzyl-aminopurine and naphthalene acetic acid. When naphthalene acetic acid was replaced by indole-3-acetic acid only multiple shoots were obtained. Pretreatment (chilling, centrifugation, reduced atmospheric pressure etc.) of the flowers was not effective but dissection of the flowers in a suspension of activated charcoal and sucrose was found essential. The anthers required an initial dark period and a high sucrose medium followed by light and lowered sucrose levels. Root tip squashes of the regenerated plantlets revealed the haploid (n = 7) nature of the plantlets.
authors.affiliation: Biochemistry Division, National Chemical Laboratory, Pune 411 008, India.
authors.forename: S
authors.initials: S
authors.lastname: Nair
authors.forename: P K
authors.initials: PK
authors.lastname: Gupta
authors.forename: A F
authors.initials: AF
authors.lastname: Mascarenhas