title: Mitochondrial DNA rearrangements in Pennisetum associated with reversion from cytoplasmic male sterility to fertility.
abstract: Endonuclease restriction fragment patterns of Pennisetum americanum L. mitochondrial DNAs (mtDNAs) from a cytoplasmic male-sterile (CMS-A1), fertile revertants and a normal fertile cytoplasm were variable, while chloroplast DNA from those lines lacked variation. Comparisons between mtDNAs of CMS-A1 (parental) and fertile revertant lines revealed the presence of a unique 4.7 kbp PstI fragment in the sterile line that was not detected in any of the revertant lines. A 9.7 kbp PstI fragment was found in all of the revertants, but not in the CMS-A1. Neither of those fragments was found in the normal cytoplasm mtDNA. Hybridization studies revealed two sets of multiple homologies: 1) the 4.7 kbp fragment had homology with a 10.9 kbp and a 13.6 kbp fragment; and 2) the 9.7 kbp fragment was homologous with the 13.6 kbp fragment. The presence of those two repeated mitochondrial sequences on the altered fragments suggests that they may be involved in the recombinational associated events with reversion from CMS to fertility in P. americanum.
authors.affiliation: Agronomy Department, University of Florida, 32611, Gainesville, FL, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Smith
authors.forename: M K
authors.initials: MK
authors.lastname: Chowdhury
authors.forename: D R
authors.initials: DR
authors.lastname: Pring