title: Guanidine bridged nucleic acid (GuNA): an effect of a cationic bridged nucleic acid on DNA binding affinity.
abstract: A novel 2',4'-BNA/LNA analog bridged by guanidine, termed as guanidine bridged nucleic acid (GuNA), was synthesized and incorporated into oligonucleotides. Thermal stabilities and nuclease resistance of GuNA-modified oligonucleotides were investigated and compared with those of 2',4'-BNA/LNA and natural DNA oligonucleotides. GuNA exhibited interestingly high binding affinity towards complementary ssDNA than 2',4'-BNA/LNA.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan. obika@phs.osaka-u.ac.jp.
authors.forename: Ajaya R
authors.initials: AR
authors.lastname: Shrestha
authors.forename: Yutaro
authors.initials: Y
authors.lastname: Kotobuki
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Hari
authors.forename: Satoshi
authors.initials: S
authors.lastname: Obika
mesh_terms: D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism; D006146:Guanidines / Q000138:chemical synthesis / Q000737:chemistry*; D009696:Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism*; D044366:Transition Temperature
chemical_list: D004277:DNA, Single-Stranded; D006146:Guanidines; D009696:Nucleic Acids; D009841:Oligonucleotides; C477371:locked nucleic acid