title: Evaluating the performance of a rapid antigen test for the detection of influenza virus in clinical specimens from children in Cameroon.
abstract: The performance of SD Bioline rapid antigen test (RAT) was evaluated using real-time reverse transcription polymerase chain reaction (rRT-PCR) as gold standard. A total of 718 nasal swabs, including 102 rRT-PCR positive and 616 rRT-PCR negative swabs, were tested. RAT demonstrates a sensitivity of 29·4% with a specificity of 100%. The positivity rate of RAT was highly associated with lower cycle threshold (Ct) values (P < 0·0001). The excellent specificity of the RAT allowed for the rapid identification of influenza cases. However, negative results should be verified by rRT-PCR test because of limitations observed in sensitivity.
authors.affiliation: National Influenza Centre, Centre Pasteur of Cameroon, Yaounde, Cameroon; Université de Yaoundé I, Yaounde, Cameroon.
authors.forename: Sebastien
authors.initials: S
authors.lastname: Kenmoe
authors.forename: Patrice
authors.initials: P
authors.lastname: Tchendjou
authors.forename: Suzie
authors.initials: S
authors.lastname: Moyo Tetang
authors.forename: Tatiana
authors.initials: T
authors.lastname: Mossus
authors.forename: Mohammed
authors.initials: M
authors.lastname: Njankouo Ripa
authors.forename: Marlène
authors.initials: M
authors.lastname: Guillet
authors.forename: Anfumbom
authors.initials: A
authors.lastname: Kfutwah
authors.forename: Richard
authors.initials: R
authors.lastname: Njouom
mesh_terms: D000293:Adolescent; D000956:Antigens, Viral / Q000032:analysis*; D002163:Cameroon; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007223:Infant; D007251:Influenza, Human / Q000175:diagnosis*; D008297:Male; D009296:Nasal Cavity / Q000821:virology*; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D019095:Point-of-Care Systems; D012680:Sensitivity and Specificity
chemical_list: D000956:Antigens, Viral
keywords: Cameroon; SD Bioline; influenza; sensitivity; specificity