title: The modulation of phosphatase expression impacts the proliferation efficiency of HSV-1 in infected astrocytes.
abstract: Herpes Simplex Virus 1 (HSV-1) is a major pathogen that causes human neurological diseases, including herpes simplex encephalitis (HSE). Previous studies have shown that astrocytes are involved in HSV-1 systemic pathogenesis in the central nervous system (CNS), although the mechanism remains unclear. In this study, a high-throughput RNAi library screening method was used to analyze the effect of host phosphatase gene regulation on HSV-1 replication using Macaca mulatta primary astrocytes in an in vitro culture system. The results showed that the downregulation of five phosphatase genes (PNKP, SNAP23, PTPRU, LOC714621 and PPM1M) significantly inhibited HSV-1 infection, suggesting that these phosphatases were needed in HSV-1 replication in rhesus astrocytes. Although statistically significant, the effect of downregulation of these phosphatases on HSV-1 replication in a human astrocytoma cell line appears to be more limited. Our results suggest that the phosphatase genes in astrocytes may regulate the immunological and pathological reactions caused by HSV-1 CNS infection through the regulation of HSV-1 replication or of multiple signal transduction pathways.
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medicine Science & Peking Union Medical College, Kunming, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yue
authors.forename: Sujie
authors.initials: S
authors.lastname: Guo
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Longding
authors.initials: L
authors.lastname: Liu
authors.forename: Qingling
authors.initials: Q
authors.lastname: Wang
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.forename: Dong
authors.initials: D
authors.lastname: Shen
authors.forename: Lichun
authors.initials: L
authors.lastname: Wang
authors.forename: Le
authors.initials: L
authors.lastname: Sun
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.forename: Yun
authors.initials: Y
authors.lastname: Liao
authors.forename: Qihan
authors.initials: Q
authors.lastname: Li
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000201:enzymology* / Q000378:metabolism / Q000821:virology*; D002478:Cells, Cultured; D000882:Haplorhini; D018259:Herpesvirus 1, Human / Q000472:pathogenicity*; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D014779:Virus Replication / Q000235:genetics / Q000502:physiology
chemical_list: D010744:Phosphoric Monoester Hydrolases