title: Overexpression, purification, and enthalpy of unfolding of ferricytochrome c552 from a psychrophilic microorganism.
abstract: The psychrophilic, hydrocarbonoclastic microorganism Colwellia psychrerythraea is important in global nutrient cycling and bioremediation. In order to investigate how this organism can live so efficiently at low temperatures (~4°C), thermal denaturation studies of a small electron transfer protein from Colwellia were performed. Colwellia cytochrome c552 was overexpressed in Escherichia coli, isolated, purified, and characterized by UV-visible absorption spectroscopy. The melting temperature (Tm) and the van't Hoff enthalpy (ΔHvH) were determined. These values suggest an unexpectedly high stability for this psychrophilic cytochrome.
authors.affiliation: Department of Chemistry, Barnard College, Columbia University, New York, NY 10027, United States.
authors.forename: Victoria F
authors.initials: VF
authors.lastname: Oswald
authors.affiliation: Department of Chemistry, Barnard College, Columbia University, New York, NY 10027, United States.
authors.forename: WeiTing
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Barnard College, Columbia University, New York, NY 10027, United States; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, United States.
authors.forename: Paul B
authors.initials: PB
authors.lastname: Harvilla
authors.affiliation: Department of Chemistry, Barnard College, Columbia University, New York, NY 10027, United States. Electronic address: jmagyar@barnard.edu.
authors.forename: John S
authors.initials: JS
authors.lastname: Magyar
mesh_terms: D003574:Cytochrome c Group / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D020563:Gammaproteobacteria / Q000201:enzymology*; D011489:Protein Denaturation; D017510:Protein Folding; D055550:Protein Stability; D013056:Spectrophotometry, Ultraviolet
chemical_list: D003574:Cytochrome c Group; C022302:cytochrome C-552
keywords: Cytochrome c(552); Psychrophile; Thermal denaturation; Van't Hoff enthalpy