title: Ploidy levels in leaf callus and regenerated plants of Solanum tuberosum determined by cytophotometric measurements of protoplasts.
abstract: In potato (Solanum tuberosum) instabilities in nuclear DNA content in callus cells of a monohaploid and a dihaploid and in tuberprogenies of a regenerated, doubled dihaploid plant were assessed by cytophotometry in isolated, purified protoplasts. Leaf protoplasts of a tetraploid variety have nuclei with 3.6±0.08 pg of DNA. In callus protoplasts a dihaploid showed 3 and a monohaploid 6 ploidy levels. Progeny of a plant, doubled and regenerated from dihaploid callus, showed mixo-euploidy, indicating an instability similar to the dihaploid callus; in addition, there are mixo-aneuploid cells.These findings confirm and extend earlier observations based on chromosome counts in other genotypes of potato. The experimental approach can be applied to a variety of other purposes.
authors.affiliation: Department of Genetics, Biological Centre, University of Groningen, Kerklaan 30, NL-9751, NN Haren, The Netherlands.
authors.forename: E
authors.initials: E
authors.lastname: Jacobsen
authors.forename: M J
authors.initials: MJ
authors.lastname: Tempelaar
authors.forename: E W
authors.initials: EW
authors.lastname: Bijmolt