title: Construction of a convenient system for easily screening inhibitors of mutated influenza virus neuraminidases.
abstract: Neuraminidase (NA) is a surface glycoprotein produced by the influenza virus. Specific NA mutations that confer resistance to anti-viral drugs have been reported. The aim of this study was to demonstrate quick preparation of the mutated NAs using the yeast surface display and its applicability for screening inhibitors. Plasmids encoding the head domain of wild-type and drug-resistant NAs were constructed and introduced into yeast, and these were successfully displayed on the yeast surface, with biochemical properties similar to the native virus NAs. This system using mutated NAs-displaying yeast provides an efficient and convenient tool for screening novel inhibitors against the drug-resistant influenza virus.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Shigemori
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Nagayama
authors.forename: Junki
authors.initials: J
authors.lastname: Yamada
authors.forename: Natsuko
authors.initials: N
authors.lastname: Miura
authors.forename: Suganya
authors.initials: S
authors.lastname: Yongkiettrakul
authors.forename: Kouichi
authors.initials: K
authors.lastname: Kuroda
authors.forename: Tohoru
authors.initials: T
authors.lastname: Katsuragi
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Ueda
keywords: Avian influenza virus H5N1; HNA, head domain of neuraminidase; Influenza A virus neuraminidase; NA, neuraminidase; Yeast surface display