title: Prolific plant regeneration from protoplast-derived tissues of Lotus corniculatus L. (birdsfoot trefoil).
abstract: Protoplasts isolated enzymatically from seedling roots, hypocotyls and cotyledons of Lotus corniculatus L. produced callus which underwent prolific shoot regeneration. The rapidity and ease of recovering plants from protoplast-derived tissues makes this forage legume an attractive experimental system for genetic manipulation.
authors.affiliation: Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, University Park, NG7 2RD, Nottingham, UK.
authors.forename: P S
authors.initials: PS
authors.lastname: Ahuja
authors.forename: S
authors.initials: S
authors.lastname: Hadiuzzaman
authors.forename: M R
authors.initials: MR
authors.lastname: Davey
authors.forename: E C
authors.initials: EC
authors.lastname: Cocking