title: Enzyme delivery using the 30Kc19 protein and human serum albumin nanoparticles.
abstract: Nanoparticles have been widely used for delivering various chemical and biomolecular drugs, such as anti-cancer drugs and therapeutic proteins. Among nanoparticles, protein nanoparticles have advantages of non-cytotoxicity and biodegradability. In this study, a recombinant 30Kc19 protein was applied to human serum albumin (HSA) nanoparticles to enhance cellular uptake and stability of a nanoparticle cargo enzyme. The 30Kc19 protein, which originates from silkworm, has cell-penetrating and enzyme-stabilizing abilities. Therefore, 30Kc19-HSA nanoparticles were expected to enhance cellular uptake and stability of an enzyme loaded on the nanoparticles. Here, nanoparticles loaded with β-galactosidase were prepared using the desolvation method. The 30Kc19-HSA nanoparticles were uniformly spherical in shape, dispersed evenly in phosphate buffered saline and cell culture media, and released β-galactosidase in a sustained manner. The 30Kc19-HSA nanoparticles had negligible toxicity to animal cells and exhibited enhanced cellular uptake and intracellular stability of β-galactosidase in HeLa and HEK293 cells when compared with those of HSA nanoparticles. These results suggest that 30Kc19-HSA protein nanoparticles could be used as a versatile tool for drug delivery to various cells.
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-744, Republic of Korea.
authors.forename: Hong Jai
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-744, Republic of Korea.
authors.forename: Hee Ho
authors.initials: HH
authors.lastname: Park
authors.affiliation: Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea.
authors.forename: Jeong Ah
authors.initials: JA
authors.lastname: Kim
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-744, Republic of Korea.
authors.forename: Ju Hyun
authors.initials: JH
authors.lastname: Park
authors.affiliation: Interdisciplinary Program for Bioengineering, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Jina
authors.initials: J
authors.lastname: Ryu
authors.affiliation: Interdisciplinary Program for Bioengineering, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Jeongseon
authors.initials: J
authors.lastname: Choi
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-744, Republic of Korea.
authors.forename: Jongmin
authors.initials: J
authors.lastname: Lee
authors.affiliation: Division of Bioengineering, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon 406-772, Republic of Korea.
authors.forename: Won Jong
authors.initials: WJ
authors.lastname: Rhee
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-744, Republic of Korea; Interdisciplinary Program for Bioengineering, Seoul National University, Seoul 151-744, Republic of Korea; Advanced Institutes of Convergence Technology, Suwon 443-270, Republic of Korea. Electronic address: thpark@snu.ac.kr.
authors.forename: Tai Hyun
authors.initials: TH
authors.lastname: Park
mesh_terms: D004337:Drug Carriers; D057809:HEK293 Cells; D006801:Humans; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D011994:Recombinant Proteins / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry*; D001616:beta-Galactosidase / Q000008:administration & dosage*
chemical_list: D004337:Drug Carriers; D011994:Recombinant Proteins; D012709:Serum Albumin; D001616:beta-Galactosidase
keywords: Drug delivery; Drug release; Enzyme stability; Protein; Protein nanoparticle