title: Improved culture ability of potato protoplasts by use of activated charcoal.
abstract: Protoplasts were obtained from in vitro grown plants of Solanum tuberosum L. The protoplasts were cultured in X-plate petri dishes with the culture medium joined to a reservoirmedium. When activated charcoal was added to the reservoirmedium the culture ability of the protoplasts was significantly increased. The effect of activated charcoal was mainly due to a less pronounced browning of the developing protoplasts and this technique might be of help in protoplast cultures where browning is a problem.
authors.affiliation: Institute of Physiological Botany, University of Uppsala, Box 540, S-751 21, Uppsala, Sweden.
authors.forename: I
authors.initials: I
authors.lastname: Carlberg
authors.forename: K
authors.initials: K
authors.lastname: Glimelius
authors.forename: T
authors.initials: T
authors.lastname: Eriksson