title: Amino-acid transport in suspension-cultured plant cells : V. Influence of L-leucine, carbon-free and nitrogen-free media on L-leucine uptake.
abstract: The carrier system which transports L-leucine (L-leu) into suspension-culturedNicotiana tabacum L. cv. Wisconsin 38 cells appeared to be constitutive since it was always present and was not induced by L-leu even in nitrogen-starved cells. However, L-leu uptake rates for cells grown in medium containing L-leu were transiently reduced as a result of either transinhibition or repression. Growth-phase cells appeared to have more L-leu carriers per unit area of membrane than stationary-phase cells, and for this reason growing-phase cells exhibit higher L-leu uptake rates. These higher rates reflect a physiological or developmental condition since growth-phase cells did not dramatically change their L-leu uptake rates when subcultured, while stationary-phase cells doubled their rates within 6 h after being subcultured. Cells grown in a medium lacking a useable carbon souce had uptake rates higher than control rates for several days. These higher rates peaked after about 1 d and then decreased over the next several days. Cells grown in a medium lacking a nitrogen souce responded similarly except that the increased rates peaked after about 3 d and persisted longer. Kinetic analysis of uptake rates in cells grown without a carbon souce for 1 d or without a nitrogen souce for 3 d indicated that the L-leu carrier had Kms similar to those of untreated cells. These results indicate that cultured tobacco cells respond to their environment by increasing or decreasing the number or activity of kinetically similar L-leu carriers.
authors.affiliation: Department of Biology, Rensselaer Polytechnic Institute, 12181, Troy, NY, USA.
authors.forename: C N
authors.initials: CN
authors.lastname: McDaniel
authors.forename: P M
authors.initials: PM
authors.lastname: Wozniak