title: Method for site-specific detection of m6A nucleoside presence in RNA based on high-resolution melting (HRM) analysis.
abstract: Chemical landscape of natural RNA species is decorated with the large number of modified nucleosides. Some of those could easily be detected by reverse transcription, while others permit only high-performance liquid chromatography or mass-spectrometry detection. Presence of m(6)A nucleoside at a particular position of long RNA molecule is challenging to observe. Here we report an easy and high-throughput method for detection of m(6)A nucleosides in RNA based on high-resolution melting analysis. The method relies on the previous knowledge of the modified nucleoside position at a particular place of RNA and allows rapid screening for conditions or genes necessary for formation of that modification.
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, Moscow 119992, Russia, Department of Bioinformatics and Bioengineering, Lomonosov Moscow State University, Moscow 119992, Russia and A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.
authors.forename: Anna Y
authors.initials: AY
authors.lastname: Golovina
authors.forename: Margarita M
authors.initials: MM
authors.lastname: Dzama
authors.forename: Kirill S
authors.initials: KS
authors.lastname: Petriukov
authors.forename: Timofei S
authors.initials: TS
authors.lastname: Zatsepin
authors.forename: Petr V
authors.initials: PV
authors.lastname: Sergiev
authors.forename: Alexey A
authors.initials: AA
authors.lastname: Bogdanov
authors.forename: Olga A
authors.initials: OA
authors.lastname: Dontsova
mesh_terms: D000241:Adenosine / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D008745:Methylation; D008780:Methyltransferases / Q000235:genetics; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015345:Oligonucleotide Probes; D012313:RNA / Q000737:chemistry* / Q000378:metabolism
chemical_list: D015345:Oligonucleotide Probes; D012313:RNA; D008780:Methyltransferases; D000241:Adenosine