title: ITP of lanthanides in microfluidic PMMA chip.
abstract: An ITP separation of eight lanthanides on a serpentine PMMA microchip with a tee junction and a 230-mm-long serpentine channel is described. The cover of the PMMA chip is 175 μm thick so that a C(4) D in microchip mode can be used to detect the lanthanides as they migrate through the microchannel. Acetate and α-hydroxyisobutyric acid are used as complexing agents to increase the electrophoretic mobility difference between the lanthanides. Eight lanthanides are concentrated within ∼ 6 min by ITP in the microchip using 10 mM ammonium acetate at pH 4.5 as the leading electrolyte and 10 mM acetic acid at ∼ pH 3.0 as the terminating electrolyte. In addition, a 2D numerical simulation of the lanthanides undergoing ITP in the microchip is compared with experimental results using COMSOL Multiphysics v4.3a.
authors.affiliation: Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USA.
authors.forename: Yongzheng
authors.initials: Y
authors.lastname: Cong
authors.forename: Danny
authors.initials: D
authors.lastname: Bottenus
authors.forename: Bingwen
authors.initials: B
authors.lastname: Liu
authors.forename: Sue B
authors.initials: SB
authors.lastname: Clark
authors.forename: Cornelius F
authors.initials: CF
authors.lastname: Ivory
mesh_terms: D000085:Acetates / Q000737:chemistry; D003198:Computer Simulation; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D006863:Hydrogen-Ion Concentration; D006885:Hydroxybutyrates / Q000737:chemistry; D028581:Lanthanoid Series Elements / Q000302:isolation & purification*; D019904:Polymethyl Methacrylate; D012815:Signal Processing, Computer-Assisted
chemical_list: D000085:Acetates; D006885:Hydroxybutyrates; D028581:Lanthanoid Series Elements; D019904:Polymethyl Methacrylate; C008039:2-hydroxyisobutyric acid; C018824:ammonium acetate
keywords: 2D simulation; COMSOL Multiphysics; ITP; Lanthanides separation; Micro- fluidics