title: New evaluation of fetal oxidative stress: measurement of the umbilical cord blood dimethyl sulfate-induced ascorbyl-free radical by an electron spin resonance method.
abstract: OBJECTIVE
  The aim is to evaluate intrapartum fetal oxidative stress in real-time by umbilical cord blood dimethyl sulfate (DMSO)-induced ascorbyl-free radical (AFR) measured by an electron spin resonance (ESR) method.
  
  
  METHODS
  Seventy-five mothers delivering at gestational age after 37 weeks were recruited. They were divided into three groups: spontaneous vaginal birth (n = 27), elective cesarean section (CS) (n = 34), and emergency CS due to non-reassuring fetal status (n = 14). Umbilical artery (UA) and venous (UV) cord blood gas analysis was performed. Serum levels of DMSO-induced AFR (AFR/DMSO) that reflect vitamin C concentrations were measured by ESR spectroscopy.
  
  
  RESULTS
  Blood gas analysis showed no significant differences among the groups. UA-AFR/DMSO level of elective CS group was significantly lower compared with spontaneous delivery group (0.32 ± 0.12 versus 0.46 ± 0.14, p < 0.005). Emergency CS group showed significantly lower levels of UA-AFR/DMSO compared with elective CS group (0.25 ± 0.11 versus 0.32 ± 0.12, p < 0.005). UV-AFR/DMSO levels had no significant difference among the groups.
  
  
  CONCLUSIONS
  It is suggested that fetal cord blood AFR/DMSO is a sensitive marker to assess fetal oxidative stress during delivery.
authors.affiliation: Department of Obstetrics and Gynecology and.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nishida
authors.forename: Shigekiyo
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Takayuki
authors.initials: T
authors.lastname: Noguchi
authors.forename: Hisashi
authors.initials: H
authors.lastname: Narahara
mesh_terms: D000328:Adult; D001774:Blood Chemical Analysis / Q000379:methods*; D002585:Cesarean Section; D003683:Dehydroascorbic Acid / Q000031:analogs & derivatives* / Q000097:blood; D004121:Dimethyl Sulfoxide; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D005316:Fetal Distress / Q000097:blood; D006801:Humans; D018384:Oxidative Stress; D011247:Pregnancy
chemical_list: C000820:semidehydroascorbic acid; D003683:Dehydroascorbic Acid; D004121:Dimethyl Sulfoxide
keywords: Ascorbyl free radial; ESR; oxidative stress; umbilical cord; vitamin C