title: Tissue-specific expression of a pea legumin gene in seeds of Nicotiana plumbaginifolia.
abstract: A 3.4-kilobase genomic DNA fragment from Pisum sativum L. containing the LegA gene, which encodes a major legumin storage protein, was transferred to Nicotiana plumbaginifolia using an Agrobacterium tumefaciens strain containing the Bin 19 binary vector system. Northern hybridisation analysis of legA-transformed plants demonstrated that legumin-specific RNA was present in developing seeds but not in developing leaves. Legumin protein was immunologically detected in the mature seeds of legA-transformed plants, and was present as the correct-size protein composed of disulphide-bonded polypeptides. It is concluded that the transferred pea genomic fragment contains all the information necessary for seed-specific expression of the legA gene, and for correct processing of the primary transcript and the precursor legumin protein.
authors.affiliation: Department of Botany, University of Durham, South Road, DH1 3LE, Durham, UK.
authors.forename: J R
authors.initials: JR
authors.lastname: Ellis
authors.forename: A H
authors.initials: AH
authors.lastname: Shirsat
authors.forename: A
authors.initials: A
authors.lastname: Hepher
authors.forename: J N
authors.initials: JN
authors.lastname: Yarwood
authors.forename: J A
authors.initials: JA
authors.lastname: Gatehouse
authors.forename: R R
authors.initials: RR
authors.lastname: Croy
authors.forename: D
authors.initials: D
authors.lastname: Boulter