title: Cytocompatibility of a silk fibroin tubular scaffold.
abstract: Regenerated silk fibroin (SF) materials are increasingly used for tissue engineering applications. In order to explore the feasibility of a novel biomimetic silk fibroin tubular scaffold (SFTS) crosslinked by poly(ethylene glycol) diglycidyl ether (PEG-DE), biocompatibility with cells was evaluated. The novel biomimetic design of the SFTS consisted of three distinct layers: a regenerated SF intima, a silk braided media and a regenerated SF adventitia. The SFTS exhibited even silk fibroin penetration throughout the braid, forming a porous layered tube with superior mechanical, permeable and cell adhesion properties that are beneficial to vascular regeneration. Cytotoxicity and cell compatibility were tested on L929 cells and human umbilical vein endothelial cells (EA.hy926). DNA content analysis, scanning electron and confocal microscopies and MTT assay showed no inhibitory effects on DNA replication. Cell morphology, viability and proliferation were good for L929 cells, and satisfactory for EA.hy926 cells. Furthermore, the suture retention strength of the SFTS was about 23N and the Young's modulus was 0.2-0.3MPa. Collectively, these data demonstrate that PEG-DE crosslinked SFTS possesses the appropriate cytocompatibility and mechanical properties for use as vascular scaffolds as an alternative to vascular autografts.
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren-ai Road, Suzhou Industrial Park, Suzhou, Jiangsu Province 215123, China. Electronic address: wangjn@suda.edu.cn.
authors.forename: Jiannan
authors.initials: J
authors.lastname: Wang
authors.forename: Yali
authors.initials: Y
authors.lastname: Wei
authors.forename: Honggen
authors.initials: H
authors.lastname: Yi
authors.forename: Zhiwu
authors.initials: Z
authors.lastname: Liu
authors.forename: Dan
authors.initials: D
authors.lastname: Sun
authors.forename: Huanrong
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002232:Carbocyanines / Q000378:metabolism; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D005349:Fibroins / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D013194:Staining and Labeling; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: C434385:3,3'-dioactadecyl-5,5'-di(4-sulfophenyl)oxacarbocyanine; D001672:Biocompatible Materials; D002232:Carbocyanines; D004247:DNA; D005349:Fibroins
keywords: Biomimetic tubular scaffold; Cytocompatibility; PEG-DE; Silk fibroin; Suture retention strength; Young's modulus