title: Barley aleurone cell development: molecular cloning of aleurone-specific cDNAs from immature grains.
abstract: The cloning of 11 different homology groups of cDNAs representing genes expressed in aleurone, but not in starchy endosperm of 20-day-old barley grains is described. Among the cDNAs, four are aleurone-specific, while the remaining are also expressed in the embryo, but not in any other part of the plant.Sequence analysis of one of the aleurone-specific clones, B11E, reveals an open reading frame coding for an unidentified 10.4 kDa protein with a putative signal sequence and a possible metal-binding finger. The B11E gene has a high GC content in the 5' leader sequence (63%), as well as in the coding region (70%) compared to known cDNAs from the barley starchy endosperm. Northern analysis of B11E indicates maximum mRNA abundance around mid-phase of grain development.When isolated immature aleurone/pericarp is incubated in tissue culture medium (MS) the B11E message disappears, indicating a requirement for a diffusible factor from the intact grain for its continued presence.
authors.affiliation: Department of Biology, Division of General Genetics, University of Oslo, PO Box 1031 Blindern, 0315, Oslo 3, Norway.
authors.forename: K
authors.initials: K
authors.lastname: Jakobsen
authors.forename: S S
authors.initials: SS
authors.lastname: Klemsdal
authors.forename: R B
authors.initials: RB
authors.lastname: Aalen
authors.forename: M
authors.initials: M
authors.lastname: Bosnes
authors.forename: D
authors.initials: D
authors.lastname: Alexander
authors.forename: O A
authors.initials: OA
authors.lastname: Olsen