title: Isolation and characterization of a thermotolerant ammonia-oxidizing bacterium Nitrosomonas sp. JPCCT2 from a thermal power station.
abstract: A thermotolerant ammonia-oxidizing bacterium strain JPCCT2 was isolated from activated sludge in a thermal power station. Cells of JPCCT2 are short non-motile rods or ellipsoidal. Molecular phylogenetic analysis of 16S rRNA gene sequences demonstrated that JPCCT2 belongs to the genus Nitrosomonas with the highest similarity to Nitrosomonas nitrosa Nm90 (100%), Nitrosomonas sp. Nm148 (99.7%), and Nitrosomonas communis Nm2 (97.7%). However, G+C content of JPCCT2 DNA was 49.1 mol% and clearly different from N. nitrosa Nm90, 47.9%. JPCCT2 was capable of growing at temperatures up to 48 °C, while N. nitrosa Nm90 and N. communis Nm2 could not grow at 42°C. Moreover, JPCCT2 grew similarly at concentrations of carbonate 0 and 5 gL(-1). This is the first report that Nitrosomonas bacterium is capable of growing at temperatures higher than 37°C.
authors.affiliation: Division of Biosphere Science, Graduate School of Environmental Science, Hokkaido University.
authors.forename: Yoshikane
authors.initials: Y
authors.lastname: Itoh
authors.forename: Keiko
authors.initials: K
authors.lastname: Sakagami
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Uchino
authors.forename: Chanita
authors.initials: C
authors.lastname: Boonmak
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Oriyama
authors.forename: Fuyumi
authors.initials: F
authors.lastname: Tojo
authors.forename: Mitsufumi
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Masaaki
authors.initials: M
authors.lastname: Morikawa
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009606:Nitrosomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D010084:Oxidation-Reduction; D010802:Phylogeny; D011210:Power Plants / Q000295:instrumentation*; D012722:Sewage / Q000032:analysis / Q000382:microbiology*
chemical_list: D012722:Sewage; D000641:Ammonia