title: Differential allelochemical detoxification mechanism in tissue cultures ofAntennaria microphylla andEuphorbia esula.
abstract: Callus and suspension cultures ofAntennaria microphylla (small everlasting) and the noxious weedEuphorbia esula (leafy spurge) can glucosylate benzene-1,4-diol (hydroquinone) to the corresponding monoglucoside, arbutin. HPLC analysis of extracts from callus tissue corroborates the presence of hydroquinone in the cells of small everlasting. Constitutive levels of a UDPG-dependent glucosyltransferase were detected in cell-free extracts of this tissue. Although this detoxification enzyme was induced in leafy spurge suspension culture cells grown in the presence of hydroquinone, the activity was six-fold lower than that measured in small everlasting. Differential ability to detoxify hydroquinone provides a basis for the observed allelopathic interaction between small everlasting and leafy spurge.
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service Western Regional Research Center, 800 Buchanan Street, 94710, Albany, California.
authors.forename: M E
authors.initials: ME
authors.lastname: Hogan
authors.forename: G D
authors.initials: GD
authors.lastname: Manners