title: Somatic embryogenesis and plant regeneration from the anther of Aconitum carmichaeli Debx.
abstract: Anthers of Aconitum carmichaeli Debx. were used for callus induction. After the addition of 5 ppm 2,4-D and 1 ppm kinetin callus induction occurred over a period of 15 weeks. When calluses were subcultured on a medium containing 1 ppm 2,4-D for 12 weeks embryogenesis occurred. Mature somatic embryos developed normal shoots when transferred to basal medium inoculated with 1 ppm GA and 5 ppm BAP. Rooting occurred after the transfer of shoots to a new medium containing 0.5 ppm IAA and plantlets formed. The transplantation of these was successful and all plants matured during 5 months subsequent cultivation.
authors.affiliation: Faculty of Pharmaceutical Sciences, Kyushu University, Higashi-ku, 812, Fukuoka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hatano
authors.forename: Y
authors.initials: Y
authors.lastname: Shoyama
authors.forename: I
authors.initials: I
authors.lastname: Nishioka