title: In vitro plantlet regeneration through asexual embryogenesis in cotyledonary segments of Corylus avellana L.
abstract: Cotyledonary nodes of corylus avellana L. excised from young seedlings cultured in K(h) medium for 5 weeks were used to induce embryoid formation. Embryogenesis was achieved in 60% of the explants over two 20-day culture steps in the presence of IBA (5 μM) plus BAP (0.5 μM) and BAP (5 μM) plus IBA (0.5 μM) respectively. Subsequent proliferation was successfully maintained during 5 subcultures on K(h) basal medium and in an unlimited number of subcultures in the presence of BAP (0.5 μM). Plant regeneration (50-55%) was easily achieved by planting into a basal K(h) medium after 10-20 days.
authors.affiliation: Departamento de Fisiología Vegetal, Facultad de Biología, Universidad de Oviedo, Oviedo, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Pérez
authors.forename: B
authors.initials: B
authors.lastname: Fernández
authors.forename: R
authors.initials: R
authors.lastname: Rodríguez