title: Phosphate solubilizing ability of Emericella nidulans strain V1 isolated from vermicompost.
abstract: Phosphorus is one of the key factors that regulate soil fertility. Its deficiencies in soil are largely replenished by chemical fertilizers. The present study was aimed to isolate efficient phosphate solubilizing fungal strains from Eisenia fetida vermicompost. Out of total 30 fungal strains the most efficient phosphate solubilizing one was Emericella (Aspergillus) nidulans V1 (MTCC 11044), identified by custom sequencing of beta-tubulin gene and BLAST analysis. This strain solubilized 13 to 36% phosphate from four different rock phosphates. After three days of incubation of isolated culture with black Mussorie phosphate rock, the highest percentage of phosphate solubilization was 35.5 +/- 1.01 with a pH drop of 4.2 +/- 0.09. Kinetics of solubilization and acid production showed a linear relationship until day five of incubation. Interestingly, from zero to tenth day of incubation, solubility of soil phosphate increased gradually from 4.31 +/- 1.57 to 13.65 +/- 1.82 (mg kg(-1)) recording a maximum of 21.23 +/- 0.54 on day 45 in respect of the V1 isolate. Further, enhanced phosphorus uptake by Phaseolus plants with significant pod yield due to soil inoculation of Emericella nidulans V1 (MTCC 11044), demonstrated its prospect as an effective biofertilizer for plant growth.
authors.affiliation: Department of Environmental Science, Tezpur University, Tezpur 784 028, India.
authors.forename: Satya Sunder
authors.initials: SS
authors.lastname: Bhattacharya
authors.forename: Soma
authors.initials: S
authors.lastname: Barman
authors.forename: Ranjan
authors.initials: R
authors.lastname: Ghosh
authors.forename: Raj Kumar
authors.initials: RK
authors.lastname: Duary
authors.forename: Linee
authors.initials: L
authors.lastname: Goswami
authors.forename: Narayan C
authors.initials: NC
authors.lastname: Mandal
mesh_terms: D000818:Animals; D001673:Biodegradation, Environmental; D018556:Crops, Agricultural / Q000187:drug effects / Q000254:growth & development; D032842:Emericella / Q000302:isolation & purification / Q000378:metabolism*; D005308:Fertilizers; D009835:Oligochaeta / Q000145:classification / Q000382:microbiology*; D027805:Phaseolus / Q000187:drug effects / Q000254:growth & development; D010710:Phosphates / Q000378:metabolism* / Q000493:pharmacokinetics / Q000494:pharmacology; D010802:Phylogeny; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012995:Solubility
chemical_list: D005308:Fertilizers; D010710:Phosphates; D012987:Soil