title: In vitro study of the cytotoxicities of two mixed-ligand oxovanadium complexes on human hepatoma cells.
abstract: The cytotoxicities of two oxovanadium complexes, VOI [VO(satsc)(phen)] (satsc = salicylaldehyde thiosemicarbazone, phen = 1,10-phenanthroline) and VOII [VO(3,5-dibrsatsc)(phen)](3,5-dibrsatsc = 3,5-dibromosalicylaldehyde thiosemicarbazone), were studied by performing MTT assays on human hepatoma cell lines BEL-7402, HUH-7 and HepG2. The results showed that both the VOI and VOII complexes possess significant anti-proliferative effects. In addition, the anti-proliferative mechanism of the complexes was analyzed by cell cycle analysis and an apoptosis assay and by detecting the mitochondrial membrane potential (delta psi m). The experimental results showed that the complexes can cause a G0/G1 phase cell cycle arrest and can significantly decrease delta psi m, causing depolarization of the mitochondrial membrane. Notably, the two complexes induced apoptosis in BEL-7402 cells and displayed typical morphological apoptotic characteristics. The cytotoxicities of the VOII complex are significantly stronger than that of the VOI complex, suggesting that the cytotoxic effects of oxovanadium complexes may be associated with the electronic effects of the complexes.
authors.affiliation: Department of Biology, School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, PR China. zyl5198@163.com
authors.forename: Yong-Li
authors.initials: YL
authors.lastname: Zhang
authors.forename: Xiang-Sheng
authors.initials: XS
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Fang
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Cai
authors.forename: Hong-Zhi
authors.initials: HZ
authors.lastname: Li
authors.forename: Jian-Wen
authors.initials: JW
authors.lastname: Mao
authors.forename: Xiao-Bao
authors.initials: XB
authors.lastname: Jin
authors.forename: Yin-Liang
authors.initials: YL
authors.lastname: Bai
authors.forename: Jia-Zheng
authors.initials: JZ
authors.lastname: Lu
mesh_terms: D000818:Animals; D017304:Annexin A5; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D004354:Drug Screening Assays, Antitumor; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D016193:G1 Phase / Q000187:drug effects; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008856:Microscopy, Fluorescence; D009942:Organometallic Compounds / Q000494:pharmacology*; D016192:Resting Phase, Cell Cycle / Q000187:drug effects; D013778:Tetrazolium Salts; D013844:Thiazoles; D014639:Vanadium / Q000494:pharmacology*
chemical_list: D017304:Annexin A5; D000970:Antineoplastic Agents; D004396:Coloring Agents; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D013778:Tetrazolium Salts; D013844:Thiazoles; D014639:Vanadium; C022616:thiazolyl blue; D016650:Fluorescein-5-isothiocyanate