title: Effect of carbon dioxide and relative humidity on self incompatibility in cauliflower, Brassica oleracea.
abstract: The events of the progamic phase of fertilization have been monitored by in vitro experiments in self compatible (SC), partial self-incompatible (PSI) and self incompatible (SI) lines. The duration of the progamic phase is about 30 h. Treatment with low concentrations of CO2 (3 to 5%) at high relative humidity (rH, 100%) had the following effects: pollen quality, which declines normally during flower ageing, was prematurely reduced; pollen adhesion and germination, both low in SI matings, were increased; the stigma callose response in SI matings was reduced to the low level of SC matings; and the number of pollen tubes in the style after SI matings significantly increased. CO2 concentrations of 4 to 6% applied for 8, 16 or 24 h at 100% rH proved to be the most effective treatment for blocking the SI response in cauliflower.
authors.affiliation: Département de Biologie Végétale, Université de Lyon 1, F-69622, Villeurbanne Cedex.
authors.forename: A
authors.initials: A
authors.lastname: Palloix
authors.forename: Y
authors.initials: Y
authors.lastname: Herve
authors.forename: R B
authors.initials: RB
authors.lastname: Knox
authors.forename: C
authors.initials: C
authors.lastname: Dumas