title: Application of Fourier transform infrared (FT-IR) spectroscopy in determination of microalgal compositions.
abstract: Fourier transform infrared spectroscopy (FT-IR) was applied in algal strain screening and monitoring cell composition dynamics in a marine microalga Isochrysis zhangjiangensis during algal cultivation. The content of lipid, carbohydrate and protein of samples determined by traditional methods had validated the accuracy of FT-IR method. For algal screening, the band absorption ratios of lipid/amide I and carbo/amide I from FT-IR measurements allowed for the selection of Isochrysis sp. and Tetraselmis subcordiformis as the most potential lipid and carbohydrate producers, respectively. The cell composition dynamics of I. zhangjiangensis measured by FT-IR revealed the diversion of carbon allocation from protein to carbohydrate and neutral lipid when nitrogen-replete cells were subjected to nitrogen limitation. The carbo/amide I band absorption ratio had also been demonstrated to depict physiological status under nutrient stress in T. subcordiformis. FT-IR serves as a tool for the simultaneous measurement of lipid, carbohydrate, and protein content in cell.
authors.affiliation: Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; College of Environment and Chemical Engineering, Dalian University, Dalian 116622, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Meng
authors.forename: Changhong
authors.initials: C
authors.lastname: Yao
authors.forename: Song
authors.initials: S
authors.lastname: Xue
authors.forename: Haibo
authors.initials: H
authors.lastname: Yang
mesh_terms: D018533:Biomass; D002241:Carbohydrates / Q000032:analysis; D008055:Lipids / Q000032:analysis; D058086:Microalgae / Q000378:metabolism*; D011506:Proteins / Q000032:analysis; D017550:Spectroscopy, Fourier Transform Infrared; D013312:Stress, Physiological; D013997:Time Factors
chemical_list: D002241:Carbohydrates; D008055:Lipids; D011506:Proteins
keywords: +N; Cell composition; DW; FT-IR; Fourier transform infrared spectroscopy; GL; Microalgae; NL; PAR; PL; PS II; Physiological status; Strain screening; TAG; TL; dry weight; glycolipid; neutral lipid; nitrogen deprivation; nitrogen repletion; phospholipid; photosynthetic active radiation; photosystem II; total lipid; triacylglycerol; −N