title: Human NKCC2 cation–Cl– co-transporter complements lack of Vhc1 transporter in yeast vacuolar membranes.
abstract: Cation–chloride co-transporters serve to transport Cl– and alkali metal cations. Whereas a large family of these exists in higher eukaryotes, yeasts only possess one cation–chloride co-transporter, Vhc1, localized to the vacuolar membrane. In this study, the human cation–chloride co-transporter NKCC2 complemented the phenotype of VHC1 deletion in Saccharomyces cerevisiae and its activity controlled the growth of salt-sensitive yeast cells in the presence of high KCl, NaCl and LiCl. A S. cerevisiae mutant lacking plasma-membrane alkali–metal cation exporters Nha1 and Ena1-5 and the vacuolar cation–chloride co-transporter Vhc1 is highly sensitive to increased concentrations of alkali–metal cations, and it proved to be a suitable model for characterizing the substrate specificity and transport activity of human wild-type and mutated cation–chloride co-transporters.
authors.forename: Silvia
authors.initials: S
authors.lastname: Petrezselyova
authors.forename: Angel
authors.initials: A
authors.lastname: Dominguez
authors.forename: Pavla
authors.initials: P
authors.lastname: Herynkova
authors.forename: Juan F
authors.initials: JF
authors.lastname: Macias
authors.forename: Hana
authors.initials: H
authors.lastname: Sychrova
mesh_terms: D027682:Cation Transport Proteins / Q000378:metabolism; D002412:Cations / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D017353:Gene Deletion; D006801:Humans; D017136:Ion Transport; D018021:Lithium Chloride / Q000378:metabolism; D010641:Phenotype; D011189:Potassium Chloride / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D012965:Sodium Chloride / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D064466:Solute Carrier Family 12, Member 1 / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D027981:Symporters / Q000235:genetics / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D027682:Cation Transport Proteins; D002412:Cations; D002712:Chlorides; C086375:ENA1 protein, S cerevisiae; C000631624:NHA1 protein, S cerevisiae; C578714:SLC12A1 protein, human; D029701:Saccharomyces cerevisiae Proteins; D017923:Sodium-Hydrogen Exchangers; D064466:Solute Carrier Family 12, Member 1; D027981:Symporters; C579356:VHC1 protein, S cerevisiae; D012965:Sodium Chloride; D011189:Potassium Chloride; D000254:Sodium-Potassium-Exchanging ATPase; D018021:Lithium Chloride