title: Analysis of microsatellite polymorphisms in South Indian patients with non syndromic cleft lip and palate.
abstract: Non syndromic cleft lip and/or palate (NSCLP) is a complex congenital anomaly with varying incidence among patients of different geographical origins. Multiple contributing factors are known to trigger the cleft formation. There are several genes involved in the aetiology of NSCLP and they are different in different populations. The genetic components of clefts that underlie the susceptibility to respond to the environment still remain unclear. In this study, five microsatellite polymorphisms from five candidate genes were employed to analyze the association between these genes and NSCLP in 83 patients and 90 controls. Genotyping was performed by separating and visualizing the fluorescently-labeled polymerase chain reaction (PCR) products. The association of the five microsatellite polymorphisms with NSCLP was tested by using the CLUMP v1.9 program that uses the Monte Carlo method. The genotypic distribution is in Hardy-Weinberg equilibrium in the control group for only the MSX1 and DLX3 genes. The RARA microsatellite was significantly associated with NSCLP. Our results suggest that the RARA gene is involved in pathogenesis of cleft lip and palate in South Indians.
authors.affiliation: Sree Balaji Dental College and Hospital, Chennai, India.
authors.forename: Dl
authors.initials: D
authors.lastname: Xavier
authors.forename: Ya
authors.initials: Y
authors.lastname: Arif
authors.forename: Rv
authors.initials: R
authors.lastname: Murali
authors.forename: S
authors.initials: S
authors.lastname: Kishore Kumar
authors.forename: S
authors.initials: S
authors.lastname: Vipin Kumar
authors.forename: R
authors.initials: R
authors.lastname: Tamang
authors.forename: K
authors.initials: K
authors.lastname: Thangaraj
authors.forename: Lvks
authors.initials: L
authors.lastname: Bhaskar
keywords: BCL3  and  EDN1  genes; Cleft palate; DLX3; MSX1; Microsatellite; Non syndromic cleft lip and/or palate (NSCLP); RARA