title: Phyllobacterium loti sp. nov. isolated from nodules of Lotus corniculatus.
abstract: Strain S658(T) was isolated from a Lotus corniculatus nodule in a soil sample obtained in Uruguay. Phylogenetic analysis of the 16S rRNA gene and atpD gene showed that this strain clustered within the genus Phyllobacterium. The closest related species was, in both cases, Phyllobacterium trifolii PETP02(T) with 99.8 % sequence similarity in the 16S rRNA gene and 96.1 % in the atpD gene. The 16S rRNA gene contains an insert at the beginning of the sequence that has no similarities with other inserts present in the same gene in described rhizobial species. Ubiquinone Q-10 was the only quinone detected. Strain S658(T) differed from its closest relatives through its growth in diverse culture conditions and in the assimilation of several carbon sources. It was not able to reproduce nodules in Lotus corniculatus. The results of DNA-DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain should be classified as a representative of a novel species of the genus Phyllobacterium, for which the name Phyllobacterium loti sp. nov. is proposed. The type strain is S658(T)( = LMG 27289(T) = CECT 8230(T)).
authors.affiliation: Laboratorio de Bioquímica, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay.
authors.forename: Maximo
authors.initials: M
authors.lastname: Sánchez
authors.affiliation: Unidad Asociada 'Grupo de Interacción Planta-Microorganismo', Universidad de Salamanca-IRNASA (CSIC), Spain.|Departamento de Desarrollo Sostenible de Sistemas Agroforestales y Ganaderos, Instituto de Recursos Naturales y Agrobiología, IRNASA, CSIC, Salamanca, Spain.
authors.forename: Martha-Helena
authors.initials: MH
authors.lastname: Ramírez-Bahena
authors.affiliation: Unidad Asociada 'Grupo de Interacción Planta-Microorganismo', Universidad de Salamanca-IRNASA (CSIC), Spain.|Departamento de Desarrollo Sostenible de Sistemas Agroforestales y Ganaderos, Instituto de Recursos Naturales y Agrobiología, IRNASA, CSIC, Salamanca, Spain.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Peix
authors.affiliation: Departamento de Desarrollo Sostenible de Sistemas Agroforestales y Ganaderos, Instituto de Recursos Naturales y Agrobiología, IRNASA, CSIC, Salamanca, Spain.
authors.forename: María J
authors.initials: MJ
authors.lastname: Lorite
authors.affiliation: Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, Granada, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Sanjuán
authors.affiliation: Departamento de Microbiología y Genética, Facultad de Farmacia, Universidad de Salamanca, Salamanca, Spain.|Unidad Asociada 'Grupo de Interacción Planta-Microorganismo', Universidad de Salamanca-IRNASA (CSIC), Spain.
authors.forename: Encarna
authors.initials: E
authors.lastname: Velázquez
authors.affiliation: Laboratorio de Bioquímica, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay.
authors.forename: Jorge
authors.initials: J
authors.lastname: Monza
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D000070116:Lotus / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D059127:Phyllobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D053204:Root Nodules, Plant / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014583:Uruguay
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S