title: Phytic acid inhibits lipid peroxidation in vitro.
abstract: Phytic acid (PA) has been recognized as a potent antioxidant and inhibitor of iron-catalyzed hydroxyl radical formation under in vitro and in vivo conditions. Therefore, the aim of the present study was to investigate, with the use of HPLC/MS/MS, whether PA is capable of inhibiting linoleic acid autoxidation and Fe(II)/ascorbate-induced peroxidation, as well as Fe(II)/ascorbate-induced lipid peroxidation in human colonic epithelial cells. PA at 100 μM and 500 μM effectively inhibited the decay of linoleic acid, both in the absence and presence of Fe(II)/ascorbate. The observed inhibitory effect of PA on Fe(II)/ascorbate-induced lipid peroxidation was lower (10-20%) compared to that of autoxidation. PA did not change linoleic acid hydroperoxides concentration levels after 24 hours of Fe(II)/ascorbate-induced peroxidation. In the absence of Fe(II)/ascorbate, PA at 100 μM and 500 μM significantly suppressed decomposition of linoleic acid hydroperoxides. Moreover, PA at the tested nontoxic concentrations (100 μM and 500 μM) significantly decreased 4-hydroxyalkenal levels in Caco-2 cells which structurally and functionally resemble the small intestinal epithelium. It is concluded that PA inhibits linoleic acid oxidation and reduces the formation of 4-hydroxyalkenals. Acting as an antioxidant it may help to prevent intestinal diseases induced by oxygen radicals and lipid peroxidation products.
authors.affiliation: Department of Biopharmacy, Medical University of Silesia, 40-055 Katowice, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Zajdel
authors.forename: Adam
authors.initials: A
authors.lastname: Wilczok
authors.forename: Ludmiła
authors.initials: L
authors.lastname: Węglarz
authors.forename: Zofia
authors.initials: Z
authors.lastname: Dzierżewicz
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D001205:Ascorbic Acid / Q000494:pharmacology; D018938:Caco-2 Cells; D006801:Humans; D007477:Ions; D007501:Iron / Q000378:metabolism; D007502:Iron Chelating Agents / Q000494:pharmacology; D008041:Linoleic Acids / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008054:Lipid Peroxides / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010833:Phytic Acid / Q000494:pharmacology*
chemical_list: D000447:Aldehydes; D007477:Ions; D007502:Iron Chelating Agents; D008041:Linoleic Acids; D008054:Lipid Peroxides; C008285:linoleic acid hydroperoxide; D010833:Phytic Acid; D007501:Iron; D001205:Ascorbic Acid