title: A lytic viral long noncoding RNA modulates the function of a latent protein.
abstract: Latent Kaposi's sarcoma-associated herpesvirus (KSHV) episomes are coated with viral latency-associated nuclear antigen (LANA). In contrast, LANA rapidly disassociates from episomes during reactivation. Lytic KSHV expresses polyadenylated nuclear RNA (PAN RNA), a long noncoding RNA (lncRNA). We report that PAN RNA promotes LANA-episome disassociation through an interaction with LANA which facilitates LANA sequestration away from KSHV episomes during reactivation. These findings suggest that KSHV may have evolved an RNA aptamer to regulate latent protein function.
authors.affiliation: Department of Dermatology, University of California, Davis, California, USA.
authors.forename: Mel
authors.initials: M
authors.lastname: Campbell
authors.forename: Kevin Y
authors.initials: KY
authors.lastname: Kim
authors.forename: Pei-Ching
authors.initials: PC
authors.lastname: Chang
authors.forename: Steve
authors.initials: S
authors.lastname: Huerta
authors.forename: Bogdan
authors.initials: B
authors.lastname: Shevchenko
authors.forename: Don-Hong
authors.initials: DH
authors.lastname: Wang
authors.forename: Chie
authors.initials: C
authors.lastname: Izumiya
authors.forename: Hsing-Jien
authors.initials: HJ
authors.lastname: Kung
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Izumiya
mesh_terms: D000956:Antigens, Viral / Q000235:genetics / Q000378:metabolism*; D006566:Herpesviridae Infections / Q000821:virology*; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000502:physiology*; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D014775:Virus Activation
chemical_list: D000956:Antigens, Viral; D009687:Nuclear Proteins; D062085:RNA, Long Noncoding; D012367:RNA, Viral; C109540:latency-associated nuclear antigen