title: Nicotiana cybrids with Petunia chloroplasts.
abstract: Protoplasts of a chloroplast-defective cultivar of Nicotiana tabacum were fused with gamma-irradiated protoplasts of Petunia hybrida. Over 100 photoautotrophic plants were regenerated; of these 94 were tested for Petunia chloroplast traits and all but one had Petunia chloroplasts based on their sensitivity to the fungal toxin, tentoxin. Chloroplast DNA was analysed for 3 of the sensitive plants and was shown to be identical to Petunia chloroplast DNA. Most of the plants (about 70%) appeared to be normal N. tabacum plants, based on morphology and chromosome number. They were fully fertile with normal pollen viability, seed set, and seed viability. The remaining 30% of the plants showed varying degrees of vegetative and reproductive abnormalities.
authors.affiliation: Department of Plant Breeding, The Swedish University of Agricultural Sciences, S-75007, Uppsala, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Glimelius
authors.forename: H T
authors.initials: HT
authors.lastname: Bonnett