title: Fabrication of nonbiofouling metal stent and in vitro studies on its hemocompatibility.
abstract: In recent years, there has been increasing interest for the surface modification of biomaterials in order to improve their surface properties. The bare metal stents surface based on 3-dimethyl(methacryloyloxyethyl)ammonium propane sulfonate polymers has shown an excellent antifouling and blood compatibility by using surface-initiated atom transfer radical polymerization. Surface structure, morphology, wettability, and element content were characterized by scanning electronic microscope, static water contact angles measurement, X-ray photoelectron spectroscopy measurement, respectively. The results showed zwitterionic brushes were successfully fabricated on bare metal stents. The blood compatibility of bare metal stents before and after modification was evaluated by platelet adhesion tests, hemolysis assay, morphological changes of red blood cells, coagulation time tests, plasma recalcification time assay, complement activation, and platelet activation at molecular level. Moreover, the cytotoxicity was also to be characterized. All assays showed after the modification with zwitterionic brush the metal stents displayed a property of excellent blood compatibility and low cytotoxicity.
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing, PR China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Miao
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing, PR China.
authors.forename: Haolin
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing, PR China maochun@njnu.edu.cn.
authors.forename: Chun
authors.initials: C
authors.lastname: Mao
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering, Nanjing University of Technology, Nanjing, China.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Chen
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D001780:Blood Coagulation Tests; D002470:Cell Survival / Q000187:drug effects; D003167:Complement Activation / Q000187:drug effects; D057809:HEK293 Cells; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D008670:Metals; D008689:Methacrylates / Q000737:chemistry* / Q000633:toxicity; D056951:Photoelectron Spectroscopy; D015539:Platelet Activation / Q000187:drug effects; D011108:Polymers / Q000737:chemistry / Q000633:toxicity; D000644:Quaternary Ammonium Compounds / Q000737:chemistry* / Q000633:toxicity; D011817:Rabbits; D015607:Stents; D013499:Surface Properties; D017155:Wettability
chemical_list: C530265:3-dimethyl(methacryloyloxyethyl)ammonium propane sulfonate; D001672:Biocompatible Materials; D008670:Metals; D008689:Methacrylates; D011108:Polymers; D000644:Quaternary Ammonium Compounds
keywords: Nonbiofouling; atom transfer radical polymerization; bare metal stents; hemocompatibility; surface modification