title: Production of solasodine by calli from different parts of Solanum eleganifolium Cav. plants.
abstract: Callus tissues from different explants (hypocotyl, cotyledon, root, leaf and fruit) of Solanum eleagnifolium Cav. were cultured on a modified Murashige-Skoog medium, with 1 mg.1(-1) 2,4-D as the sole growth regulator. The presence of the alkaloid solasodine was determined by spectrophotometric and TLC methods. Its concentration ranged from 1.00 to 2.15 mg.g(-1) DW. The calli from different explants showed a direct association between the solasodine production and their growth, although they have a different production rate. It was also observed that about the seventh week of culture the metabolite concentration decreased in all cases.
authors.affiliation: Departamento de Tecnología Farmacéutica, Biotecnología y Microbiología Industrial, Junín 956, 1113, Buenos Aires, Argentina.
authors.forename: H M
authors.initials: HM
authors.lastname: Nigra
authors.forename: O H
authors.initials: OH
authors.lastname: Caso
authors.forename: A M
authors.initials: AM
authors.lastname: Giulietti