title: Discrimination between the red- and far-red-absorbing forms of phytochrome from Avena sativa L. by monoclonal antibodies.
abstract: A set of rat monoclonal antibodies (ARC MAC 48 to 52 and 54 to 56), raised to phytochrome from dark-grown seedlings of Avena sativa L. was tested for the ability to discriminate between the red-absorbing (Pr) and far-red-absorbing (Pfr) forms of phytochrome by indirect enzyme-linked immunosorbent assay. MAC 50 bound more strongly to Pfr and MAC 49 and 52 showed preferential binding to Pr from extracts of dark-grown Avena seedlings; MAC 50 also bound more strongly to Pfr from brushite-purified phytochrome. The remainder of the monoclonal antibodies and a rabbit polyclonal antiphytochrome preparation did not discriminate between Pr and Pfr. The results provide evidence for conformational changes in defined regions of the phytochrome apoprotein upon photoconversion.
authors.affiliation: Plant Physiology Department, Glasshouse Crops Research Institute, Littlehampton, West Sussex, UK.
authors.forename: B
authors.initials: B
authors.lastname: Thomas
authors.forename: S E
authors.initials: SE
authors.lastname: Penn
authors.forename: G W
authors.initials: GW
authors.lastname: Butcher
authors.forename: G
authors.initials: G
authors.lastname: Galfre