title: Organophosphate pesticides-induced changes in the redox status of rat tissues and protective effects of antioxidant vitamins.
abstract: Organophosphates (OPs) pesticides are among the most toxic synthetic chemicals purposefully added in the environment. The common use of OP insecticides in public health and agriculture results in an environmental pollution and a number of acute and chronic poisoning events. Present study was aimed to evaluate the potential of monocrotophos and quinalphos to effect the redox status and glutathione (GSH) homeostasis in rat tissues and find out whether antioxidant vitamins have some protection on the pesticide-induced alterations. The results showed that these pesticides alone or in combination, caused decrease in the levels of GSH and the corresponding increase in the levels of GSSG, decreasing the GSH/GSSG ratio. The results also showed that NADPH/NADP(+) and NADH/NAD(+) ratios were decreased in the liver and brain of rats on exposure with mococrotophos, quinalphos, and their mixture. These pesticides, alone or in combination, caused alterations in the activities of GSH reductase and glucose-6-phosphate dehydrogenase in the rat tissues. However, the expression of the GSH recycling enzymes did not show significant alterations as compared to control. From the results, it can be concluded that these pesticides generate oxidative stress but their effects were not synergistic when given together and prior feeding of antioxidant vitamins tend to reduce the toxicities of these pesticides.
authors.affiliation: School of Studies in Biochemistry, Jiwaji University, Gwalior 474 011, India.
authors.forename: Vibhuti
authors.initials: V
authors.lastname: Mishra
authors.forename: Nalini
authors.initials: N
authors.lastname: Srivastava
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D008297:Male; D009243:NAD / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000633:toxicity*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D010575:Pesticides / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D014815:Vitamins / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D009943:Organophosphorus Compounds; D010575:Pesticides; D014815:Vitamins; D009243:NAD; D005954:Glucosephosphate Dehydrogenase; D005978:Glutathione
keywords: GSH/GSSG ratio; antioxidant vitamins; monocrotophos; quinalphos; redox imbalancer