title: Double-strand breaks on F98 glioma rat cells induced by minibeam and broad-beam synchrotron radiation therapy.
abstract: OBJECTIVE
  To assess the DNA damage induced by MBRT and BB radiations on glioma cells.
  
  
  METHODS
  The analysis of fluorescent intensity emitted per nucleus was plotted versus DNA content 2 and 17 h after irradiations. At around cell-doubling time (17 h) after exposures, the remaining DNA radiation damage could be correlated with cellular death.
  
  
  RESULTS
  A higher γH2AX IF intensity per cell could be detected 2 and 17 h after MBRT when compared with BB. 17 h after MBRT, misrepaired damaged cells remained arrested in both G1 and G2 phases.
  
  
  CONCLUSIONS
  A pronounced G2 phase arrest was detected at 17 h after MBRT and BB. However, only after MBRT, a dose-dependent increasing number of damaged cells appeared arrested also in the G1 phase, and a higher amount of cells more prone to undergo apoptosis were detected. The threshold dose required to enhance the effectiveness of both synchrotron radiation techniques was 12 Gy.
authors.affiliation: Centre d'Estudis en Biofísica (CEB), Faculty of Medicine, Universitat Autònoma de Barcelona, Bellaterra (Catalonia), Spain, sigidu@gmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Gil
authors.forename: Y
authors.initials: Y
authors.lastname: Prezado
authors.forename: M
authors.initials: M
authors.lastname: Sabés
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D053903:DNA Breaks, Double-Stranded / Q000528:radiation effects*; D005910:Glioma / Q000235:genetics*; D011878:Radiotherapy / Q000379:methods*; D051381:Rats; D017356:Synchrotrons