title: Serum albumin in 2D: a Langmuir monolayer approach.
abstract: Understanding of protein interaction at the molecular level raises certain difficulties which is the reason a model membrane system such as the Langmuir monolayer technique was developed. Ubiquitous proteins such as serum albumin comprise 50% of human blood plasma protein content and are involved in many biological functions. The important nature of this class of protein demands that it be studied in detail while modifying the experimental conditions in two dimensions to observe it in all types of environments. While different from bulk colloidal solution work, the two dimensional approach allows for the observation of the interaction between molecules and subphase at the air-water interface. Compiled in this review are studies which highlight the characterization of this protein using various surroundings and also observing the types of interactions it would have when at the biomembrane interface. Free-energy changes between molecules, packing status of the bulk analyte at the interface as well as phase transitions as the monolayer forms a more organized or aggregated state are just some of the characteristics which are observed through the Langmuir technique. This unique methodology demonstrates the chemical behavior and physical behavior of this protein at the phase boundary throughout the compression of the monolayer.
authors.affiliation: Department of Chemistry, Cox Science Center, Room 315, University of Miami, 1301 Memorial Drive, Coral Gables, FL 33146, USA. Electronic address: n.crawford1@umiami.edu.
authors.forename: Nicholas F
authors.initials: NF
authors.lastname: Crawford
authors.affiliation: Department of Chemistry, Cox Science Center, Room 315, University of Miami, 1301 Memorial Drive, Coral Gables, FL 33146, USA. Electronic address: rml@miami.edu.
authors.forename: Roger M
authors.initials: RM
authors.lastname: Leblanc
mesh_terms: D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics / Q000379:methods* / Q000639:trends; D002417:Cattle; D006801:Humans; D008954:Models, Biological; D010743:Phospholipids / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D000075462:Serum Albumin, Human; D055672:Static Electricity; D013499:Surface Properties
chemical_list: C422455:ALB protein, human; D010743:Phospholipids; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D000075462:Serum Albumin, Human
keywords: Bovine serum albumin; Human serum albumin; Langmuir monolayer; Langmuir–Blodgett films; Mixed monolayers