title: Dietary flavonoids bind to mono-ubiquitinated annexin A1 in nuclei, and inhibit chemical induced mutagenesis.
abstract: In order to investigate the mechanisms of anti-mutagenic action by dietary flavonoids, we investigated if they inhibit mutation of the thymidine kinase (tk) gene in L5178Ytk(±) lymphoma cells. Silibinin, quercetin and genistein suppressed mutation of the tk gene induced in L5178Ytk(±) lymphoma cells by methyl methanesulfonate (MMS) and As(3+). Flavone and flavonol were less effective. To establish that mutation of the tk gene in L5178Ytk(±) lymphoma cells by MMS and As(3+) is mediated through mono-ubiquitinated annexin A1, L5178Ytk(±) lymphoma cells were treated with annexin A1 anti-sense oligonucleotide. The treatment reduced mRNA as well as protein levels of annexin A1, and suppressed mutation of the tk gene. Nuclear extracts from L5178Ytk(±) lymphoma cells catalyzed translesion DNA synthesis with an oligonucleotide template containing 8-oxo-guanosine in an annexin A1 dependent manner. This translesion DNA synthesis was inhibited by the anti-mutagenic flavonoids, silibinin, quercetin and genistein, in a concentration dependent manner, but only slightly by flavone and flavonol. Because these observations implicate involvement of annexin A1 in mutagenesis, we examined if flavonoids suppress nuclear annexin A1 helicase activity. Silibinin, quercetin and genistein inhibited ssDNA binding, DNA chain annealing and DNA unwinding activities of purified nuclear mono-ubiquitinated annexin A1. Flavone and flavonol were ineffective. The apparent direct binding of anti-mutagenic flavonoids to the annexin A1 molecule was supported by fluorescence quenching. Taken together, these findings illustrate that nuclear annexin A1 may be a novel and productive target protein of prevention for DNA damage induced gene mutation, ultimately conferring cancer chemoprevention.
authors.affiliation: Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, United States. Electronic address: fhirata@wayne.edu.
authors.forename: Fusao
authors.initials: F
authors.lastname: Hirata
authors.affiliation: Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, United States.
authors.forename: Takasuke
authors.initials: T
authors.lastname: Harada
authors.affiliation: Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, United States.
authors.forename: George B
authors.initials: GB
authors.lastname: Corcoran
authors.affiliation: Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, United States.
authors.forename: Aiko
authors.initials: A
authors.lastname: Hirata
mesh_terms: D017305:Annexin A1 / Q000378:metabolism*; D016587:Antimutagenic Agents / Q000494:pharmacology*; D002467:Cell Nucleus / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis; D005419:Flavonoids / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D016296:Mutagenesis / Q000187:drug effects; D013937:Thymidine Kinase / Q000235:genetics; D054875:Ubiquitination
chemical_list: D017305:Annexin A1; D016587:Antimutagenic Agents; D005419:Flavonoids; D004247:DNA; D013937:Thymidine Kinase
keywords: 8-oxo-G; 8-oxo-guanosine; Annexin A1; Chemoprevention; DMSO; DNA helicase; Flavonoids; MMS; Mutation; TFT; dimethylsulfoxide; methyl methansulfonate; thymidine kinase; tk; trifluoro-thymidine