title: In vitro cultures of Cinchona species : Precursor feeding of C. ledgeriana root organ suspension cultures with L-Tryptophan.
abstract: The uptake of L-[methylene-(14)C]-tryptophan from culture medium into root organs of Cinchona ledgeriana and the subsequent incorporation of the radiolabel into quinine and quinidine is reported. In addition, feeding unlabelled L-tryptophan at levels of 500mg/l to the cultures results in a 5-fold increase in the yields of both quinoline alkaloids.
authors.affiliation: Department of Pharmacognosy, The School of Pharmacy, University of London, 29-39 Brunswick Square, WC1N 1AX, London, Great Britain.
authors.forename: C A
authors.initials: CA
authors.lastname: Hay
authors.forename: L A
authors.initials: LA
authors.lastname: Anderson
authors.forename: M F
authors.initials: MF
authors.lastname: Roberts
authors.forename: J D
authors.initials: JD
authors.lastname: Phillipson