title: Involvement of sulphur nutrition in modulating iron deficiency responses in photosynthetic organelles of oilseed rape (Brassica napus L.).
abstract: The aim of this study was to characterize the roles of sulphur (S) nutrition in modulating the responses to iron (Fe) deficiency in the photosynthetic organelles of oilseed rape. Eight-week-old plants grown hydroponically were fed with S-sufficient or S-deprived solution with or without Fe(III)-EDTA. Responses to four S and Fe combined treatments were analysed after 5 and 10 days. Leaf chlorosis was generated by either S- or Fe-deprivation, with a decrease in chlorophyll and carotenoid content. These negative effects were more severe in the absence of S. The expression of Fe²⁺ transporter (IRT1) and Fe(III) chelate reductase (FRO1) gene was induced for the first 5 days and decreased after 10 days in the S-deprived roots, but largely improved by S supply even in the absence of Fe. Lack of ferric chelate reducing activity in the Fe-deprived roots in the absence of S was largely improved by S supply. The activity of photosynthesis, RuBisCO and sucrose synthase was closely related to S status in leaves. Electron microscopic observation showed that the Fe-deficiency in the absence of S greatly resulted in a severe disorganisation of thylakoid lamellae with loss of grana. However, these impacts of Fe-deficiency were largely restored in the presence of S. The present results indicate that S nutrition has significant role in ameliorating the damages in photosynthetic apparatus caused by Fe-deficiency.
authors.affiliation: Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture and Life Science, Chonnam National University, Buk-Gwangju, P.O. Box 205, Gwangju, 500-600, Korea.
authors.forename: Sowbiya
authors.initials: S
authors.lastname: Muneer
authors.forename: Bok-Rye
authors.initials: BR
authors.lastname: Lee
authors.forename: Kil-Yong
authors.initials: KY
authors.lastname: Kim
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Tae-Hwan
authors.initials: TH
authors.lastname: Kim
mesh_terms: D029688:Brassica napus / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D002338:Carotenoids / Q000378:metabolism; D027682:Cation Transport Proteins / Q000235:genetics; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism / Q000648:ultrastructure; D038181:FMN Reductase / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005964:Glucosyltransferases / Q000378:metabolism; D007501:Iron / Q000378:metabolism* / Q000494:pharmacology; D015388:Organelles / Q000378:metabolism; D010788:Photosynthesis; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000502:physiology; D054046:Plant Stomata / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism* / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D027682:Cation Transport Proteins; D010940:Plant Proteins; D002734:Chlorophyll; D002338:Carotenoids; D013455:Sulfur; D007501:Iron; D038181:FMN Reductase; C023903:ferric citrate iron reductase; D005964:Glucosyltransferases; C022699:sucrose synthase; D012273:Ribulose-Bisphosphate Carboxylase