title: The improvement of enzymatic hydrolysis efficiency of rape straw and Miscanthus giganteus polysaccharides.
abstract: The research was carried out with the aim to determine the impact of various combinations of cellulase and hemicellulase preparations on the effectiveness of enzymatic hydrolysis of polysaccharides of rape straw and Miscanthus giganteus after alkaline pretreatment. Their effectiveness was evaluated based on the quantity of saccharides released during enzymatic reaction and yield calculated in respect of the sum of polysaccharides present in native substrates. The complex of preparations produced from Trichoderma longibrachiatum fungi turned out to be the most effective. The study demonstrated a significant effect of xylanases from T. longibrachiatum, the presence of which evoked a 27-45% increase in the effectiveness of polysaccharides hydrolysis compared to the enzymatic complexes without their addition. In addition, results achieved in this study confirmed the necessity of applying the pretreatment in lignocellulose substrates conversion into bioethanol.
authors.affiliation: Chair of Food Biotechnology, University of Warmia and Mazury in Olsztyn, Heweliusza 1, 10-718 Olsztyn, Poland. Electronic address: karolina.swiatek@uwm.edu.pl.
authors.forename: Karolina
authors.initials: K
authors.lastname: Swiątek
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Lewandowska
authors.forename: Magdalena
authors.initials: M
authors.lastname: Swiątek
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Bednarski
authors.forename: Bartosz
authors.initials: B
authors.lastname: Brzozowski
mesh_terms: D000704:Analysis of Variance; D029741:Brassica rapa / Q000378:metabolism*; D002480:Cellulase / Q000378:metabolism*; D006868:Hydrolysis / Q000187:drug effects; D008031:Lignin / Q000378:metabolism; D006109:Poaceae / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D012972:Sodium Hydroxide / Q000494:pharmacology
chemical_list: D011134:Polysaccharides; C036909:lignocellulose; D012972:Sodium Hydroxide; D008031:Lignin; D002480:Cellulase
keywords: Cellulase; Enzymatic hydrolysis; Lignocellulose; Miscanthus giganteus; Rape straw