title: The Rhizobium - legume symbiosis: observation of root infection by bright-field microscopy after staining with methylene blue.
abstract: Staining of infected legume roots with 0.01% methylene blue facilitated the observation of the initial steps of the Rhizobium-legume symbiosis. It allowed particularly the visualization by bright-field microscopy of infection threads in the root hairs and the root cortex of the host plant.
authors.affiliation: Institut de Cytologie et de Biologie Cellulaire, Faculté des Sciences de Marseille-Luminy, L.A. CNRS 179, F-13288, Marseille Cedex 9, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Vasse
authors.forename: G L
authors.initials: GL
authors.lastname: Truchet