title: Genotyping of human parechoviruses in Iranian young children with aseptic meningitis and sepsis-like illness.
abstract: Human parechoviruses (HPeV) are classified into 14 genotypes. HPeV1 and HPeV2 are the most prevalent genotypes in young children, which have been associated with mild to severe diseases. This study was conducted to investigate the involvement of HPeVs in aseptic meningitis and sepsis-like illness in Iran. Viral RNA was extracted from 148 cerebrospinal fluid samples from children <8 years old with primary diagnosis of aseptic meningitis and/or sepsis-like illness. Specific HPeV, HEV real-time PCR and HPeV typing were done to identify the infection rate of these viruses. HPeV and HEV were detected in 64 (43.24 %), 31 (20.94 %) of 148 patients with 10 (6.75 %) coinfection. VP1/VP3 junction region was successfully sequenced from 12 of the HPeV-positive specimens, and all of them were identified as HPeV1. HPeV was more prevalent than HEV in both aseptic meningitis and sepsis-like illness, so further studies are needed to understand the disease burden of HPeV infections, and clinical manifestations especially in specific illnesses of possible viral etiology. Direct detection of these viruses leads to reduce hospitalization and use of antibiotic, which are often followed by other complications in neonates and young children.
authors.affiliation: Department of Hepatitis and AIDS, Pasteur Institute of Iran, Enghelab Square, 12 Faravrdin Ave, Tehran, Iran, prahimi@pasteur.ac.ir.
authors.forename: Pooneh
authors.initials: P
authors.lastname: Rahimi
authors.forename: Hakimeh Mahdian
authors.initials: HM
authors.lastname: Naser
authors.forename: Seyed Davar
authors.initials: SD
authors.lastname: Siadat
authors.forename: Amir
authors.initials: A
authors.lastname: Sohrabi
authors.forename: Ehsan
authors.initials: E
authors.lastname: Mostafavi
authors.forename: Mahdieh
authors.initials: M
authors.lastname: Motamedirad
authors.forename: Golnaz
authors.initials: G
authors.lastname: Bahramali
authors.forename: Seyed Mehdi
authors.initials: SM
authors.lastname: Sadat
authors.forename: Mehdi Shafiee
authors.initials: MS
authors.lastname: Ardestani
mesh_terms: D002648:Child; D002675:Child, Preschool; D003937:Diagnosis, Differential; D004770:Enterovirus / Q000235:genetics* / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000175:diagnosis* / Q000821:virology; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007492:Iran; D008297:Male; D008582:Meningitis, Aseptic / Q000175:diagnosis* / Q000821:virology; D058889:Molecular Typing; D030061:Parechovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000175:diagnosis* / Q000821:virology; D012367:RNA, Viral / Q000145:classification / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D018805:Sepsis / Q000175:diagnosis* / Q000821:virology
chemical_list: D012367:RNA, Viral