title: Solasodine production in rapidly proliferating tissue cultures of Solanum laciniatum ait.
abstract: Callus and suspension cultures, established from seedling and leaf explants of Solanum laciniatum Ait were analysed for solasodine using a spectrophotometric assay. Solasodine concentration in both types of culture ranged from 0.5 - 1.0 mg/g dry wt., with a small number of callus explants containing higher concentrations. There was no overall fall in concentration as a result of serial subculture, and in suspension cultures the level remained constant throughout a single passage. Solasodine concentration was enhanced by the induction of organogenesis in both primary leaf explants and callus. ABA, added at 0.04 mg 1(-1), increased solasodine yield in callus cultures whilst CEPA, at concentrations of 10 mg 1(-1) and higher, inhibited production. Dark grown callus contained significantly more solasodine than light grown.
authors.affiliation: Department of Plant Biology, University of Birmingham, B15 2TT, Birmingham, UK.
authors.forename: S
authors.initials: S
authors.lastname: Chandler
authors.forename: J
authors.initials: J
authors.lastname: Dodds