title: Vaccination of cattle with a recombinant bivalent toxoid against botulism serotypes C and D.
abstract: Cattle botulism is a fatal intoxication caused by botulinum neurotoxins (BoNTs) produced by Clostridium botulinum serotypes C and D resulting in economic losses. Vaccination is the most effective way to control botulism. However, the commercially available vaccines are difficult and hazardous to produce. Neutralizing antibodies against the C-terminal fragment of the BoNT heavy chain (HC) are known to protect against lethal doses of BoNTs. We report the vaccination of cattle with a previously tested recombinant chimera consisting of Escherichia coli heat-labile enterotoxin B subunit and the HC of BoNTs C and D. Vaccinated animals produced neutralizing antibodies against serotypes C and D averaging 5±0 and 6.14±1.06IU/mL, respectively. For BoNT D, the titers were greater than those measured for the commercial vaccine, which induced titers of 5±0 and 2.85±1.35 against the respective serotypes, suggesting that this chimera is effective against cattle botulism.
authors.affiliation: Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Universidade Federal de Pelotas, CP 354, CEP 96010-900 Pelotas, RS, Brazil.
authors.forename: Carlos E P
authors.initials: CE
authors.lastname: Cunha
authors.affiliation: Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Universidade Federal de Pelotas, CP 354, CEP 96010-900 Pelotas, RS, Brazil.
authors.forename: Gustavo M S G
authors.initials: GM
authors.lastname: Moreira
authors.affiliation: Escola de Veterinária, Universidade Federal de Minas Gerais, CP 567, CEP 30123-970 Belo Horizonte, MG, Brazil.
authors.forename: Felipe M
authors.initials: FM
authors.lastname: Salvarani
authors.affiliation: Escola de Veterinária, Universidade Federal de Minas Gerais, CP 567, CEP 30123-970 Belo Horizonte, MG, Brazil.
authors.forename: Monique S
authors.initials: MS
authors.lastname: Neves
authors.affiliation: Escola de Veterinária, Universidade Federal de Minas Gerais, CP 567, CEP 30123-970 Belo Horizonte, MG, Brazil.
authors.forename: Francisco C F
authors.initials: FC
authors.lastname: Lobato
authors.affiliation: Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Universidade Federal de Pelotas, CP 354, CEP 96010-900 Pelotas, RS, Brazil.
authors.forename: Odir A
authors.initials: OA
authors.lastname: Dellagostin
authors.affiliation: Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Universidade Federal de Pelotas, CP 354, CEP 96010-900 Pelotas, RS, Brazil. Electronic address: fabricio.rochedo@ufpel.edu.br.
authors.forename: Fabricio R
authors.initials: FR
authors.lastname: Conceição
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D057134:Antibodies, Neutralizing / Q000097:blood; D001427:Bacterial Toxins / Q000276:immunology; D001428:Bacterial Vaccines / Q000627:therapeutic use*; D001905:Botulinum Toxins / Q000276:immunology*; D001906:Botulism / Q000517:prevention & control / Q000662:veterinary*; D002417:Cattle / Q000276:immunology*; D003014:Clostridium botulinum; D004768:Enterotoxins / Q000276:immunology; D029968:Escherichia coli Proteins / Q000276:immunology; D008297:Male; D011994:Recombinant Proteins / Q000276:immunology; D014121:Toxoids / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D057134:Antibodies, Neutralizing; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D014121:Toxoids; C096325:botulinum toxin type D; C027538:heat-labile enterotoxin, E coli; D001905:Botulinum Toxins; C096324:botulinum toxin type C
keywords: Botulinum neurotoxins; Clostridium botulinum; Fused antigens; LTB; Recombinant vaccine