title: Differential accumulation of potato tuber mRNAs during the hypersensitive response induced by arachidonic acid elicitor.
abstract: Accumulation of messenger RNAs in potato tuber discs was analysed during the hypersensitive response induced by treatment with the biotic elicitor arachidonic acid. In vitro translation of polysomal poly(A)(+) RNAs indicated that the accumulation of some sixteen mRNAs varied following treatment with arachidonic acid, and that the level of thirteen of these was increased. Two cDNA closes (pSTH-1 and-2) were isolated from a library of elicitor-treated tissue cDNAs. Northern blot analysis using these clones as molecular probes indicated that the levels of at least two mRNAs were markedly increased after elicitor treatment. In hybrid-released translation experiments, each of the cDNA clones selected more than one mRNA. Translation of these mRNAs yielded two polypeptides of Mr 45 000 (for the pSTH-1 clone), and three polypeptides of Me 17 000 (for the pSTH-2 clone). The low molecular weight polypeptides may correspond to potato pathogenesis-related (PR) proteins.
authors.affiliation: Department of Biochemistry, University of Montreal, Station A, P.O. Box 6128, H3C 3J7, Montreal, Quebec, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Marineau
authors.forename: D P
authors.initials: DP
authors.lastname: Matton
authors.forename: N
authors.initials: N
authors.lastname: Brisson