title: Analyses of mitochondrial amino acid sequence datasets support the proposal that specimens of Hypodontus macropi from three species of macropodid hosts represent distinct species.
abstract: BACKGROUND
  Hypodontus macropi is a common intestinal nematode of a range of kangaroos and wallabies (macropodid marsupials). Based on previous multilocus enzyme electrophoresis (MEE) and nuclear ribosomal DNA sequence data sets, H. macropi has been proposed to be complex of species. To test this proposal using independent molecular data, we sequenced the whole mitochondrial (mt) genomes of individuals of H. macropi from three different species of hosts (Macropus robustus robustus, Thylogale billardierii and Macropus [Wallabia] bicolor) as well as that of Macropicola ocydromi (a related nematode), and undertook a comparative analysis of the amino acid sequence datasets derived from these genomes.
  
  
  RESULTS
  The mt genomes sequenced by next-generation (454) technology from H. macropi from the three host species varied from 13,634 bp to 13,699 bp in size. Pairwise comparisons of the amino acid sequences predicted from these three mt genomes revealed differences of 5.8% to 18%. Phylogenetic analysis of the amino acid sequence data sets using Bayesian Inference (BI) showed that H. macropi from the three different host species formed distinct, well-supported clades. In addition, sliding window analysis of the mt genomes defined variable regions for future population genetic studies of H. macropi in different macropodid hosts and geographical regions around Australia.
  
  
  CONCLUSIONS
  The present analyses of inferred mt protein sequence datasets clearly supported the hypothesis that H. macropi from M. robustus robustus, M. bicolor and T. billardierii represent distinct species.
authors.affiliation: Faculty of Veterinary Science, The University of Melbourne, Parkville, Melbourne, Victoria 3010, Australia. jabbara@unimelb.edu.au.
authors.forename: Abdul
authors.initials: A
authors.lastname: Jabbar
authors.forename: Ian
authors.initials: I
authors.lastname: Beveridge
authors.forename: Namitha
authors.initials: N
authors.lastname: Mohandas
authors.forename: Neil B
authors.initials: NB
authors.lastname: Chilton
authors.forename: D Timothy J
authors.initials: DT
authors.lastname: Littlewood
authors.forename: Aaron R
authors.initials: AR
authors.lastname: Jex
authors.forename: Robin B
authors.initials: RB
authors.lastname: Gasser
mesh_terms: D000818:Animals; D001315:Australia; D001499:Bayes Theorem; D004275:DNA, Ribosomal / Q000235:genetics; D054629:Genome, Mitochondrial; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics; D007614:Macropodidae / Q000469:parasitology*; D024101:Mitochondrial Proteins / Q000737:chemistry / Q000235:genetics; D010802:Phylogeny; D013320:Strongyloidea / Q000145:classification* / Q000201:enzymology / Q000235:genetics*
chemical_list: D004275:DNA, Ribosomal; D015801:Helminth Proteins; D024101:Mitochondrial Proteins