title: Interaction of molybdenum with human erythrocyte membrane proteins.
abstract: This study describes the interaction of molybdenum with blood components. Molybdenum-99 was added to blood, and after four washings, 3% of the total radioactivity was found in red cells. More specifically, the radioactivity was determined to be associated with the cell membrane.Molybdenum-99 in the +VI form did not interact with the human erythrocyte membrane; however, Mo(V) forms did interact. Of five different compounds, the highes uptake was observed with a brown Mo(V)-ascorbate complex generated from Mo(VI) and ascorbic acid in the molar ratio 1∶20. A membrane suspension of Mo-ascorbate-treated human erythrocytes was prepared and the solubilized proteins were separated on a polyacrylamide gel in the presence of sodium dodecyl sulfate (SDS). Molybdenum-99 binding to spectrin was demonstrated, as well as some minor interactions with membrane hemoglobin and bands 6 and 8.
authors.affiliation: Institute of Hematology and Blood Transfusion, Prague.
authors.forename: M
authors.initials: M
authors.lastname: Kselíková
authors.forename: T
authors.initials: T
authors.lastname: Mařík
authors.forename: B
authors.initials: B
authors.lastname: Bíbr
authors.forename: J
authors.initials: J
authors.lastname: Lener