title: Genetic transformation of flax (Linum usitatissimum) by Agrobacterium tumefaciens: regeneration of transformed shoots via a callus phase.
abstract: Genetic transformation of flax (Linum usitatissimum) has been achieved using an A. tumefaciens strain carrying a non-oncogenic Ti plasmid-derived vector containing a chimaeric npt-II gene and a wild type nopaline synthase gene. Fertile, transformed shoots were most easily obtained from Km(r) callus developing on hypocotyl sections. The totipotency of the Km(r) callus was dependent upon its origin. T-DNA was visualised by Southern blotting in all Km(r) tissues. Efficient expression of nopaline synthase and the chimaeric npt-II gene was found in transformed Km(r) callus and regenerated shoots.
authors.affiliation: Department of Botany, University of Leicester, University Road, LE1 7RH, Leicester, UK.
authors.forename: N
authors.initials: N
authors.lastname: Basiran
authors.forename: P
authors.initials: P
authors.lastname: Armitage
authors.forename: R J
authors.initials: RJ
authors.lastname: Scott
authors.forename: J
authors.initials: J
authors.lastname: Draper