title: Characterisation of the Egeria densa Planch. leaf symplast : Inhibition of the intercellular movement of fluorescent probes by group II ions.
abstract: The hydrophyllic dyes fluorescein glutamic acid, fluorescein glutamylglutamic acid (F(Glu)2), fluorescein hexaglycine, fluorescein leucyldiglutamyl-leucine and 6-carboxyfluorescein are unable to pass the plasmalemma in leaves of E. densa. However, when injected into single cells the dye conjugates of molecular weight 665 dalton or less move freely from cell-to-cell. This intercellular movement presumably occurs via the plant symplast. Movement of F(Glu)2 from the injected cell occurs with greatly reduced frequency when Ca(2+), Mg(2+) or Sr(2+) are injected into the cell immediately prior to the dye. The fraction of dye injections leading to movement declines with increasing group II ion concentration in the electrode tip, up to 10 mM. Sodium and K ions do not affect dye movement. When dye injection is delayed 30 min after Ca(2+) injection, dye movement is no longer inhibited. Thus the cells recover from the Ca(2+) injection, indicating that the ion does not cause major cell damage. Recovery from Mg(2+) injection is not complete within 60 min. Treatment of leaves with chemicals expected to raise the concentration of free intracellular group II ions, notably the mitochondrial uncoupler carbonyl cyanide p-trifluoromethoxyphenyl hydrazone, the inhibitor of mitochondrial Ca(2+) uptake trifluralin, or the ionophore A23187 also inhibits dye movement, while the calmodulin inhibitor trifluoperazine does not. Cytoplasmic streaming is inhibited by Ca(2+) or Mg(2+) injection and by the metabolic inhibitors. However when streaming is stopped by cytochalasin B, dye movement is not inhibited. Hence steaming is not necessary for dye movement. Thus the cytoplasmic concentration of free group II ions may directly regulate the permeability of the plant symplast.
authors.affiliation: Department of Agronomy and Horticultural Science, University of Sydney, 2006, N.S.W., Australia.
authors.forename: M G
authors.initials: MG
authors.lastname: Erwee
authors.forename: P B
authors.initials: PB
authors.lastname: Goodwin