title: Aggregation pheromone of driedfruit beetle,Carpophilus hemipterus Wind-tunnel bioassay and identification of two novel tetraene hydrocarbons.
abstract: A male-produced aggregation pheromone was demonstrated inCarpophilus hemipterus (L.) (Coleoptera: Nitidulidae) using a wind-tunnel bioassay. Both sexes responded to the pheromone, but the beetles flew in the wind tunnel only after they had been starved for at least several hours. The attractiveness of the pheromone was greatly enhanced by volatiles from a food source, and combinations of pheromone and food volatiles typically attracted 3-10 times more beetles than either source by itself. A variety of food-related sources of volatiles were effective. These included apple juice; a mixture of baker's yeast plus banana; the pinto bean diet used for rearing this beetle; the chemicals propyl acetate, ethanol; and a mixture of acetaldehyde, ethyl acetate, and ethanol. The pheromonal activity resided with a series of 10 male-specific, unsaturated hydrocarbons of 13, 14, and 15 carbon atoms. These were partially separated by HPLC. No single compound was absolutely required for pheromonal activity to be observed, and various subsets of these compounds were active. The most abundant component was (2E,4E,6E,8E)-3,5,7-trimethyl-2,4,6,8-decatetraene. One minor component was (2E,4E,6E,8E)-3,5,7-trimethyl-2,4,6,8-undecatetraene. These structures were proven by synthesis. Together, the synthetic compounds were as active in the wind tunnel as the beetle-derived pheromone.
authors.affiliation: USDA Agricultural Research Service, Northern Regional Research Center, 1815 N. University St., 61604, Peoria, Illinois.
authors.forename: R J
authors.initials: RJ
authors.lastname: Bartelt
authors.forename: P F
authors.initials: PF
authors.lastname: Dowd
authors.forename: R D
authors.initials: RD
authors.lastname: Plattner
authors.forename: D
authors.initials: D
authors.lastname: Weisleder