title: Study on nuclear and cytoplasmic genome expression in wheat by two-dimensional gel electrophoresis : 1. First results on 18 alloplasmic lines.
abstract: In this first analysis the protein patterns obtained by two-dimensional gel electrophoresis of 8 day-old leaves from 18 alloplasmic wheat lines are compared. From 440 spots retained on the basis of their reproducibility, 36 proteins were observed to vary in different cytoplasms, allowing us to distinguish the T. aestivum cytoplasm from 5 Aegilops cytoplasms. Twenty-four of the 36 variable proteins could be structurally related to the large subunit of RuBPCase. Nuclear variation between 3 wheat varieties was observed for 14 proteins.
authors.affiliation: Laboratoire d'Amélioration des Plantes-G.I.S. Moulon, Bat. 360, Université de Paris-Sud, F-91405, Orsay, France.
authors.forename: M
authors.initials: M
authors.lastname: Zivy
authors.forename: H
authors.initials: H
authors.lastname: Thiellement
authors.forename: D
authors.initials: D
authors.lastname: de Vienne
authors.forename: J P
authors.initials: JP
authors.lastname: Hofmann