title: Rhabdomyolysis-induced acute kidney injury under hypoxia and deprivation of food and water.
abstract: BACKGROUND
  To investigate the renal pathophysiologyin rhabdomyolysis-induced acute kidney injury (AKI) in rats under hypoxia and deprivation of food and water (HDFW), thus broadening the knowledge about rhabdomyolysis-induced AKI in massive earthquake.
  
  
  METHODS
  Male Wistar rats weighing 200-230g were randomized into control, rhabdomyolysis (R), HDFW and rhabdomyolysis in combination with HDFW (R/HDFW) group. Experimental rhabdomyolysis rat model was established through clamping hind limb muscles, HDFW model rats were kept in 10% hypoxic chamber unavailable to food and water. At 1, 3, 5, 7, 9, 11d after treatment, serum creatinine (Scr) level, renal index, renal structural changes and cell apoptosis were analyzed.
  
  
  RESULTS
  After R, HDFW, R/HDFW treatment, the animals showed significantly higher Scr levels than the control group. Renal index in R and R/HDFW groups elevated remarkably compared with that in control and HDFW group. The results of histopathology, ultra-structure and apoptosis assay suggested that rhabdomyolysis caused renal tubular injury, HDFW treatment resulted in renal vascular dilation, tissue congestion and tubular cell damage. In addition, more severe renal lesion appeared in R/HDFW.
  
  
  CONCLUSIONS
  We conclude that the association of experimental rhabdomyolysis with HDFW results in a different functional and histological pattern. The rhabdomyolysis-HDFW combination causes more severe renal injury.
authors.affiliation: Beijing Institute of Radiation Medicine.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Wang
authors.forename: Dewen
authors.initials: D
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zuo
authors.forename: Shaoxia
authors.initials: S
authors.lastname: Wang
authors.forename: Xinping
authors.initials: X
authors.lastname: Xu
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Guo
authors.forename: Yabing
authors.initials: Y
authors.lastname: Gao
authors.forename: Shuiming
authors.initials: S
authors.lastname: Wang
authors.forename: Ruiyun
authors.initials: R
authors.lastname: Peng
mesh_terms: D058186:Acute Kidney Injury / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D005508:Food Deprivation / Q000502:physiology*; D000860:Hypoxia / Q000150:complications / Q000473:pathology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012206:Rhabdomyolysis / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D014868:Water Deprivation / Q000502:physiology*