title: Isolation of cycloeucalenol from Boophone disticha and evaluation of its cytotoxicity.
abstract: Boophone disticha (Amaryllidaceae) is widely used in traditional medicine in southern Africa. Several alkaloids, volatile oils and fatty acids have been isolated from the plant. However, there has been no literature report of a triterpene from B. disticha. Cycloeucalenol, a cycloartane triterpene, together with its regio-isomer, was isolated from the ethyl acetate extract of the bulbs using column chromatography and preparative thin layer chromatography. Structural elucidation was carried out using 1D and 2D NMR and mass spectroscopy. The MTT and neutral red assays were used to assess the cytotoxicity of the compound in human neuroblastoma (SH-SY5Y) cells. The compound was obtained as a mixture of two regio-isomers, which were separated for the first time by chromatographic optimization. Integration of the 1H NMR spectrum showed that cycloeucalenol and its regio-isomer were present in a ratio of 1.04:1. A dose-dependent decrease in cell viability was observed using both cytotoxicity assays. IC51 values of 173.0 +/- 5.1 microM and 223.0 +/- 6.4 microM were obtained for the MTT and neutral red assays, respectively, indicative of the low toxicity of the compound. This work describes for the first time, the presence of triterpene compounds from the genus Boophone.
authors.affiliation: Department of Pharmacology, Faculty of Health Sciences, University of Pretoria, Private Bag X323, Arcadia 0007, South Africa. adewusiadekanmi@gmail.com
authors.forename: Emmanuel Adekanmi
authors.initials: EA
authors.lastname: Adewusi
authors.forename: Paul
authors.initials: P
authors.lastname: Steenkamp
authors.forename: Gerda
authors.initials: G
authors.lastname: Fouche
authors.forename: Vanessa
authors.initials: V
authors.lastname: Steenkamp
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007536:Isomerism; D019668:Liliaceae / Q000737:chemistry*; D010840:Phytosterols / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010840:Phytosterols; C007198:cycloleucalenol