title: Whole-body vibrations do not elevate the angiogenic stimulus when applied during resistance exercise.
abstract: Knowledge about biological factors involved in exercise-induced angiogenesis is to date still scanty. The present study aimed to investigate the angiogenic stimulus of resistance exercise with and without superimposed whole-body vibrations. Responses to the exercise regimen before and after a 6-week training intervention were investigated in twenty-six healthy male subjects. Serum was collected at the initial and final exercise sessions and circulating levels of matrix metalloproteinases (MMP) -2 and -9, Vascular Endothelial Growth Factor (VEGF) and endostatin were determined via ELISA. Furthermore, we studied the proliferative effect of serum-treated human umbilical vein endothelial cells in vitro via BrdU-incorporation assay. It was found that circulating MMP-2, MMP-9, VEGF and endostatin levels were significantly elevated (P<0.001) from resting levels after both exercise interventions, with higher post-exercise VEGF concentrations in the resistance exercise (RE) group compared to the resistive vibration exercise (RVE) group. Moreover, RE provoked increased endothelial cell proliferation in vitro and higher post-exercise circulating endostatin concentrations after 6 weeks of training. These effects were elusive in the RVE group. The present findings suggest that resistance exercise leads to a transient rise in circulating angiogenic factors and superimposing vibrations to this exercise type might not further trigger a potential signaling of angiogenic stimulation in skeletal muscle.
authors.affiliation: German Aerospace Center, Institute of Aerospace Medicine and Space Physiology, Cologne, Germany ; German Sport University Cologne, Department of Molecular and Cellular Sport Medicine, Cologne, Germany.
authors.forename: Åsa
authors.initials: Å
authors.lastname: Beijer
authors.forename: André
authors.initials: A
authors.lastname: Rosenberger
authors.forename: Birgit
authors.initials: B
authors.lastname: Bölck
authors.forename: Frank
authors.initials: F
authors.lastname: Suhr
authors.forename: Jörn
authors.initials: J
authors.lastname: Rittweger
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Bloch
mesh_terms: D000328:Adult; D043925:Angiogenesis Inducing Agents / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation; D043169:Endostatins / Q000097:blood / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism / Q000502:physiology; D015444:Exercise / Q000502:physiology*; D005081:Exercise Therapy / Q000379:methods; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000097:blood / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000097:blood / Q000378:metabolism; D018482:Muscle, Skeletal / Q000098:blood supply / Q000378:metabolism / Q000502:physiology; D018919:Neovascularization, Physiologic / Q000502:physiology*; D005082:Physical Exertion / Q000502:physiology*; D012146:Rest / Q000502:physiology; D042461:Vascular Endothelial Growth Factor A / Q000097:blood / Q000378:metabolism; D014732:Vibration
chemical_list: D043925:Angiogenesis Inducing Agents; D043169:Endostatins; D042461:Vascular Endothelial Growth Factor A; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9