title: Mangrovibacterium diazotrophicum gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from a mangrove sediment, and proposal of Prolixibacteraceae fam. nov.
abstract: A nitrogen-fixing bacterium, designated strain SCSIO N0430(T), was isolated from a mangrove sediment sample. Analysis of the sequence of the nifH gene responsible for nitrogen fixation in this strain indicated a close relationship to an uncultured bacterium ZNZ-D11 (GenBank accession no. JF896696). 16S rRNA gene sequence analysis revealed that this isolate had less than 93 % similarity to its closest relative, Sunxiuqinia elliptica DQHS4(T). A phylogenetic tree reconstructed based on 16S rRNA gene sequences revealed that strain SCSIO N0430(T) was a member of the phylum Bacteroidetes. Chemotaxonomic and physiological characteristics, including phospholipids and major fatty acids, readily distinguished the isolate from established members of the phylum Bacteroidetes. It is concluded that strain SCSIO N0430(T) represents a novel genus and species, for which the name Mangrovibacterium diazotrophicum gen. nov., sp. nov. is proposed, with the type strain of the species SCSIO N0430(T) ( = KCTC 32129(T) = DSM 27148(T) = JCM 19152(T)). Based on phylogenetic characteristics and 16S rRNA gene signature nucleotide patterns, the three genera Sunxiuqinia, Prolixibacter and Mangrovibacterium are proposed to make up a novel family, Prolixibacteraceae fam. nov., in the order Bacteroidales.
authors.affiliation: Graduate University of the Chinese Academy of Sciences, Beijing 100049, PR China.|CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, CAS; RNAM Center for Marine Microbiology, CAS; Guangdong Key Laboratory of Marine Materia Medica; and South China Sea Institute of Oceanology, CAS, Guangzhou 510301, PR China.
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Huang
authors.affiliation: Graduate University of the Chinese Academy of Sciences, Beijing 100049, PR China.|CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, CAS; RNAM Center for Marine Microbiology, CAS; Guangdong Key Laboratory of Marine Materia Medica; and South China Sea Institute of Oceanology, CAS, Guangzhou 510301, PR China.
authors.forename: Yu Juan
authors.initials: YJ
authors.lastname: Liu
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, CAS; RNAM Center for Marine Microbiology, CAS; Guangdong Key Laboratory of Marine Materia Medica; and South China Sea Institute of Oceanology, CAS, Guangzhou 510301, PR China.
authors.forename: Jun-De
authors.initials: JD
authors.lastname: Dong
authors.affiliation: First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, PR China.
authors.forename: Ling-Yun
authors.initials: LY
authors.lastname: Qu
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, CAS; RNAM Center for Marine Microbiology, CAS; Guangdong Key Laboratory of Marine Materia Medica; and South China Sea Institute of Oceanology, CAS, Guangzhou 510301, PR China.
authors.forename: Yan-Ying
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, CAS; RNAM Center for Marine Microbiology, CAS; Guangdong Key Laboratory of Marine Materia Medica; and South China Sea Institute of Oceanology, CAS, Guangzhou 510301, PR China.
authors.forename: Fa-Zuo
authors.initials: FZ
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, CAS; RNAM Center for Marine Microbiology, CAS; Guangdong Key Laboratory of Marine Materia Medica; and South China Sea Institute of Oceanology, CAS, Guangzhou 510301, PR China.
authors.forename: Xin-Peng
authors.initials: XP
authors.lastname: Tian
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, CAS; RNAM Center for Marine Microbiology, CAS; Guangdong Key Laboratory of Marine Materia Medica; and South China Sea Institute of Oceanology, CAS, Guangzhou 510301, PR China.
authors.forename: Si
authors.initials: S
authors.lastname: Zhang
mesh_terms: D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation; D010088:Oxidoreductases / Q000235:genetics; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D053833:Wetlands
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7; D010088:Oxidoreductases; C029130:nitrogenase reductase