title: [Establishment of a rabbit model of cardiopulmonary bypass in acute cerebral embolism phase].
abstract: OBJECTIVE
  To establish a stable and feasible rabbit model of cardiopulmonary bypass (CPB) in acute cerebral embolism phase for studying the effects of CPB on brain tissues and the timing of surgical intervention of acute cerebral embolism.
  
  
  METHODS
  Fifty-four rabbits were randomized into group A (n=18) to receive CPB without middle cerebral artery occlusion (MCAO) and group B to undergo CPB at 24 h (group B1, n=18) or 1 week (group B2, n=18) after MCAO. Through a supraorbital margin approach, electrocoagulation was carried out to occlude the main stem of the left MCA under direct vision to establish MCAO. Magnetic resonance imaging (MRI) was performed at both 24 h and 1 week after MCAO, and the severity of cerebral embolization was evaluated. CPB was established by cannulation of the ascending aorta and the right atrium through a median sternotomy incision. MRI was performed at 2 h after CPB to observe the brain tissues.
  
  
  RESULTS
  MCAO was successfully established in groups B1 and B2, and all the rabbits survived after MCAO. In both groups A and B, MRI examination detected no cerebral hemorrhage or new embolism 2 h after CPB.
  
  
  CONCLUSIONS
  We have established a stable and feasible CPB model in rabbits with acute cerebral embolism to allow study of the mechanisms of CPB-related organ damage and its interventions.
authors.affiliation: Department of Cardiothoracic Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.E-mail: lcxtzz06b03@126.com.
authors.forename: Wenkui
authors.initials: W
authors.lastname: Mo
authors.forename: Liangyong
authors.initials: L
authors.lastname: He
authors.forename: Qunqing
authors.initials: Q
authors.lastname: Chen
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Yan
authors.forename: Jian
authors.initials: J
authors.lastname: Tong
authors.forename: Hua
authors.initials: H
authors.lastname: Meng
authors.forename: Fuli
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002315:Cardiopulmonary Bypass; D004195:Disease Models, Animal; D004564:Electrocoagulation; D005260:Female; D020244:Infarction, Middle Cerebral Artery / Q000209:etiology / Q000503:physiopathology; D008279:Magnetic Resonance Imaging; D008297:Male; D020768:Middle Cerebral Artery / Q000601:surgery; D011817:Rabbits; D011897:Random Allocation