title: Use of PKA-mediated phenotypes for genetic and small-molecule screens in Schizosaccharomyces pombe.
abstract: PKA (protein kinase A) in the fission yeast Schizosaccharomyces pombe controls transcription of genes involved in metabolism, cell growth and sexual development. In the present review, we discuss phenotypes associated with either high or low PKA activity in the context of how they can be used to carry out genetic or small-molecule screens that affect components of the PKA pathway. Although our recent research has focused on the study of heterologously expressed cyclic nucleotide PDEs (phosphodiesterases), these same methods can be used to target other S. pombe proteins or their functionally equivalent orthologues that act in the PKA pathway.
authors.affiliation: *Biology Department, Boston College, Chestnut Hill, MA 02467, U.S.A.
authors.forename: Ana Santos
authors.initials: AS
authors.lastname: de Medeiros
authors.forename: Alexander
authors.initials: A
authors.lastname: Magee
authors.forename: Kyle
authors.initials: K
authors.lastname: Nelson
authors.forename: Liora
authors.initials: L
authors.lastname: Friedberg
authors.forename: Karolina
authors.initials: K
authors.lastname: Trocka
authors.forename: Charles S
authors.initials: CS
authors.lastname: Hoffman
mesh_terms: D017868:Cyclic AMP-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D029702:Schizosaccharomyces pombe Proteins; D017868:Cyclic AMP-Dependent Protein Kinases