title: Molecular analysis of γ-gliadin gene families at the complex Gli-1 locus of bread wheat (T. aestivum L.).
abstract: A cDNA clone (pTag1436) carrying a complete coding sequence for a γ-gliadin polypeptide has been identified and sequenced. By hybridisation to size fractionated poly A(+) RNA from wheat nullisomic-tetrasomic lines, homologous transcripts from the Gli-A1, Gli-B1 and Gli-D1 loci were identified. These mRNAs differed from those complementary to a low molecular weight (LMW) glutenin cDNA clone. Hybridization of pTag1436 to digested wheat DNA produced a pattern of fragments unrelated to that obtained using a LMW glutenin cDNA probe. These results indicate that the γ-gliadin and LMW glutenin families, although both located at the Gli-1 loci, are distinct by hybridisation.
authors.affiliation: Plant Breeding Institute, Maris Lane, CB2 2LQ, Trumpington, Cambridge, UK.
authors.forename: D
authors.initials: D
authors.lastname: Bartels
authors.forename: I
authors.initials: I
authors.lastname: Altosaar
authors.forename: N P
authors.initials: NP
authors.lastname: Harberd
authors.forename: R F
authors.initials: RF
authors.lastname: Barker
authors.forename: R D
authors.initials: RD
authors.lastname: Thompson