title: The effect of pH and lysosomal extract on rat liver metallothionein (Cd-thionein).
abstract: Was studied in vitro. The(109)Cd-labeled protein was isolated by gel filtration and incubated with a lysosomal extract from the same source. No degradation was seen when the pH of the incubation medium was 5 or higher, whereas the degradation of Cd-thionein was completed in 2 h at a pH of 4.5. Dissociation of Cd(2+) from thionein, which takes place to a fair extent at a pH below 5, appears necessary before the enzymatic breakdown of the thionein moiety can take place.
authors.affiliation: Department of Veterinary Pathology, Faculty of Veterinary Medicine, State University, PO Box 80.158, 3508 TD, Utrecht, The Netherlands.
authors.forename: H
authors.initials: H
authors.lastname: Meulenaar
authors.forename: C J
authors.initials: CJ
authors.lastname: van den Hamer
authors.forename: T S
authors.initials: TS
authors.lastname: van den Ingh
authors.forename: T M
authors.initials: TM
authors.lastname: Stolk