title: RLCC-isolation of Raucaffricine from its most efficient source - cell suspension cultures of Rauwolfia serpentina Benth.
abstract: Raucaffricine (vomilenine-galactoside) was shown to be the major indole alkaloid of Rauwolfia serpentina Benth. cell suspension cultures grown in AP-medium (alkaloid production medium). Several grams of this glycoalkaloid can conveniently be isolated by RLCC (Rotation Locular Countercurrent Chromatography). A newly discovered enzyme efficiently converts the glycoalkaloid to its aglycon, vomilenine, which occupies a key function in the biosynthesis of ajmaline. This is the first demonstration of the occurrence of raucaffricine in Rauwolfia serpentina.
authors.affiliation: Lehrstuhl Pharmazeutische Biologie, Ludwig-Maximilians-Universität, Karlstr. 29, D-8000, München, Federal Republic of Germany.
authors.forename: H
authors.initials: H
authors.lastname: Schübel
authors.forename: J
authors.initials: J
authors.lastname: Stöckigt