title: Curcumin inhibits the SOS response induced by levofloxacin in Escherichia coli.
abstract: The role of RecA protein in bacterial resistance to antibiotics makes this protein attractive from a pharmacological point of view. In this study we demonstrate that curcumin is able to inhibit the SOS response in Escherichia coli induced by levofloxacin. The blaTEM-1 gene has been placed under the control of the LexA-binding box and used as reporter gene. The expression of TEM-1 β-lactamase enzyme was increased in the presence of ssDNA induced by levofloxacin, while, the presence of curcumin at 8μg/ml, reduced dramatically the expression of the reporter gene. Moreover a simple microplate assay suitable for high-throughput screening has been developed.
authors.affiliation: Department of Biotechnological and Applied Clinical Sciences, University of l'Aquila, Italy.
authors.forename: Pierangelo
authors.initials: P
authors.lastname: Bellio
authors.affiliation: Department of Biotechnological and Applied Clinical Sciences, University of l'Aquila, Italy.
authors.forename: Fabrizia
authors.initials: F
authors.lastname: Brisdelli
authors.affiliation: Department of Biotechnological and Applied Clinical Sciences, University of l'Aquila, Italy.
authors.forename: Mariagrazia
authors.initials: M
authors.lastname: Perilli
authors.affiliation: Department of Biotechnological and Applied Clinical Sciences, University of l'Aquila, Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Sabatini
authors.affiliation: Department of Biotechnological and Applied Clinical Sciences, University of l'Aquila, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Bottoni
authors.affiliation: Department of Biotechnological and Applied Clinical Sciences, University of l'Aquila, Italy.
authors.forename: Bernardetta
authors.initials: B
authors.lastname: Segatore
authors.affiliation: Department of Biotechnological and Applied Clinical Sciences, University of l'Aquila, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Setacci
authors.affiliation: Department of Biotechnological and Applied Clinical Sciences, University of l'Aquila, Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Amicosante
authors.affiliation: Department of Biotechnological and Applied Clinical Sciences, University of l'Aquila, Italy. Electronic address: celenza@univaq.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Celenza
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins; D003474:Curcumin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli; D017930:Genes, Reporter; D064704:Levofloxacin / Q000494:pharmacology*; D013014:SOS Response, Genetics / Q000187:drug effects*; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003474:Curcumin; D064704:Levofloxacin; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein
keywords: Bacterial SOS response; Curcumin; Levofloxacin