title: Formation of anti-plant viral protein by Mirabilis jalapa L. cells in suspension culture.
abstract: The extract of Mirabilis jalapa cultured cells and its precipitate fraction with 90% saturated ammonium sulfate showed an anti-plant viral activity comparable to that of the roots and leaves of the original plant. In the immunodiffusion experiment, the extract of cultured cells positively reacted with MAP (Mirabilis Anti-plant viral Protein) anti-serum. The changes in MAP formation during cell growth and the MAP content of roots and leaves were examined using enzyme-linked immunosorbent assay (ELISA). MAP formation proceeded almost in parallel with cell growth. The MAP content of cultured cells reached the highest level (0.6 mg/g dry weight) on the 9th day after inoculation, which was less than one-third of the content of the roots but three times larger than that of the leaves.
authors.affiliation: Central Research Institute, Japan Tobacco Inc., 6-2 Umegaoka, Midori-ku, 227, Yokohama, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ikeda
authors.forename: Y
authors.initials: Y
authors.lastname: Takanami
authors.forename: S
authors.initials: S
authors.lastname: Imaizumi
authors.forename: T
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Mikami
authors.forename: S
authors.initials: S
authors.lastname: Kubo