title: Determination of triamcinolone acetonide in silicone oil and aqueous humor of vitrectomized rabbits' eyes: Application for a pharmacokinetic study with intravitreal triamcinolone acetonide injections (Kenalog® 40).
abstract: A simple and accurate method including liquid-liquid extraction and protein precipitation procedures from silicone oil and aqueous humor samples followed by high-performance liquid chromatography (HPLC-UV) was developed and validated to determine the pharmacokinetic profile of triamcinolone acetonide in silicone oil and aqueous humor of rabbits' eyes submitted to the pars plana vitrectomy surgery. The method was successfully applied to quantify the drug remaining in silicone oil and aqueous humor (LOQ range of 1μg/mL). The triamcinolone acetonide remained in silicone oil and aqueous humor of vitrectomized rabbits' eyes for four weeks after the intravitreal injections.
authors.affiliation: Faculty of Pharmacy of the Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address: gabriellafcunha@gmail.com.
authors.forename: Gabriella M
authors.initials: GM
authors.lastname: Fernandes-Cunha
authors.affiliation: Faculty of Pharmacy of the Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Juliana B
authors.initials: JB
authors.lastname: Saliba
authors.affiliation: Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, Ribeirao Preto School of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Rubens C
authors.initials: RC
authors.lastname: Siqueira
authors.affiliation: Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, Ribeirao Preto School of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Jorge
authors.affiliation: Faculty of Pharmacy of the Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Armando
authors.initials: A
authors.lastname: Silva-Cunha
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000737:chemistry* / Q000378:metabolism*; D059625:Liquid-Liquid Extraction; D008297:Male; D011817:Rabbits; D012827:Silicone Oils / Q000737:chemistry*; D014222:Triamcinolone Acetonide / Q000737:chemistry* / Q000493:pharmacokinetics*; D014821:Vitrectomy
chemical_list: D012827:Silicone Oils; D014222:Triamcinolone Acetonide
keywords: HPLC-UV; Intravitreal injections; Silicone oil; Triamcinolone acetonide; Vitrectomy