title: Field-amplified sample stacking β-cyclodextrin modified capillary electrophoresis for quantitative determination of diastereomeric saponins.
abstract: Successful simultaneous diastereomeric separation and sensitive determination of two pairs of triterpenoidal saponins have been achieved by capillary electrophoresis (CE) using β-cyclodextrin (β-CD) as a stereoselective agent to cooperate with borate complexation. A usual technique for isolation and group separation of saponins was developed as an appropriate purification step prior to the determination of individual saponins by CE. Soyasaponin I ( S1: ), azukisaponin V ( S2: ), bersimoside I ( S3: ) and bersimoside II ( S4: ) could be well separated within 14 min in a fused-silica capillary (60 cm long to the detector with an additional 10 cm to the cathode; 75 µm i.d.). The background electrolyte was borate buffer (80 mM, pH 10), containing 24 mM β-CD. The separation voltage was 14 kV with a detection wavelength of 195 nm. The sample was electrokinetically injected using a voltage of 16 kV for 12 s. Methanol (70%) was used as the diluent for field-amplified sample stacking after hydrodynamic injection of short water plug (5 cm, 4 s). The method was partially validated for linearity, repeatability, reproducibility, limits of detection and limits of quantification. The correlation coefficients of the calibration curves were all >0.998, and the recoveries were from 98.23 to 96.21%.
authors.affiliation: Faculty of Pharmacy, Pharmaceutical Chemistry Department, Misr International University, Km 28 Ismailia Road, Cairo, Egypt emara_miu@yahoo.com.
authors.forename: Samy
authors.initials: S
authors.lastname: Emara
authors.affiliation: P.I. Laboratory Single Cell MS, RIKEN Quantitative Biology Center, 6-2-3, Furuedai, Suita, Osaka 565-0874, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Masujima
authors.affiliation: Faculty of Pharmacy, Pharmaceutical Chemistry Department, Misr International University, Km 28 Ismailia Road, Cairo, Egypt.
authors.forename: Walaa
authors.initials: W
authors.lastname: Zarad
authors.affiliation: Faculty of Pharmacy, Pharmacognosy Department, Assiut University, Assiut 71526, Egypt.
authors.forename: Khaled
authors.initials: K
authors.lastname: Mohamed
authors.affiliation: Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Modern Sciences and Arts University, 26 July Mehwar Road Intersection with Wahat Road, 6 October City, Egypt.
authors.forename: Maha
authors.initials: M
authors.lastname: Kamal
authors.affiliation: Faculty of Pharmacy, Pharmaceutical Chemistry Department, Cairo University, Kasr El Aini St., Cairo 11562, Egypt.
authors.forename: Marwa
authors.initials: M
authors.lastname: Fouad
authors.affiliation: Faculty of Pharmacy, Pharmaceutical Chemistry Department, Cairo University, Kasr El Aini St., Cairo 11562, Egypt.
authors.forename: Ramzia
authors.initials: R
authors.lastname: EL-Bagary
mesh_terms: D001881:Borates / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D016014:Linear Models; D010936:Plant Extracts / Q000737:chemistry; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis* / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D029921:Trifolium / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D001881:Borates; D010936:Plant Extracts; D012503:Saponins; D047392:beta-Cyclodextrins