title: In vitro and in vivo antioxidant activities of polysaccharide purified from aloe vera (Aloe barbadensis) gel.
abstract: The in vitro and in vivo antioxidant potentials of a polysaccharide isolated from aloe vera gel were investigated. Enzymatic extracts were prepared from aloe vera gel by using ten digestive enzymes including five carbohydrases and five proteases. Among them, the highest yield was obtained with the Viscozyme extract and the same extract showed the best radical scavenging activity. An active polysaccharide was purified from the Viscozyme extract using ethanol-added separation and anion exchange chromatography. Purified aloe vera polysaccharide (APS) strongly scavenged radicals including DPPH, hydroxyl and alkyl radicals. In addition, APS showed a protective effect against AAPH-induced oxidative stress and cell death in Vero cells as well as in the in vivo zebrafish model. In this study, it is proved that both the in vitro and in vivo antioxidant potentials of APS could be further utilized in relevant industrial applications.
authors.affiliation: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea; Jeju Aloe Agriculture Association Cooperation, Sehwa-ri, Pyosun-myeon, Seogwipo, Jeju-do 690-1, Republic of Korea.
authors.forename: Min-Cheol
authors.initials: MC
authors.lastname: Kang
authors.forename: Seo Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Yoon Taek
authors.initials: YT
authors.lastname: Kim
authors.forename: Eun-A
authors.initials: EA
authors.lastname: Kim
authors.forename: Seung-Hong
authors.initials: SH
authors.lastname: Lee
authors.forename: Seok-Chun
authors.initials: SC
authors.lastname: Ko
authors.forename: W A J P
authors.initials: WA
authors.lastname: Wijesinghe
authors.forename: Kalpa W
authors.initials: KW
authors.lastname: Samarakoon
authors.forename: Young-Sun
authors.initials: YS
authors.lastname: Kim
authors.forename: Jin Hun
authors.initials: JH
authors.lastname: Cho
authors.forename: Hyeang-Su
authors.initials: HS
authors.lastname: Jang
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Jeon
mesh_terms: D000504:Aloe / Q000737:chemistry*; D000578:Amidines / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D002852:Chromatography, Ion Exchange; D004625:Embryo, Nonmammalian / Q000187:drug effects; D006026:Glycoside Hydrolases / Q000737:chemistry; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors; D007527:Isoenzymes / Q000737:chemistry; D009097:Multienzyme Complexes / Q000737:chemistry; D016877:Oxidants / Q000037:antagonists & inhibitors / Q000494:pharmacology; D010447:Peptide Hydrolases / Q000737:chemistry; D010851:Picrates / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D014709:Vero Cells; D015027:Zebrafish / Q000502:physiology
chemical_list: D000578:Amidines; D000975:Antioxidants; D001713:Biphenyl Compounds; D007527:Isoenzymes; D009097:Multienzyme Complexes; D016877:Oxidants; D010851:Picrates; D010936:Plant Extracts; D011134:Polysaccharides; C578134:Viscozyme L, Aspergillus; D017665:Hydroxyl Radical; C046728:2,2'-azobis(2-amidinopropane); C004931:1,1-diphenyl-2-picrylhydrazyl; D006026:Glycoside Hydrolases; C019822:carbohydrase; D010447:Peptide Hydrolases
keywords: Aloe vera; Antioxidant activity; Oxidative stress; Polysaccharide; Reactive oxygen species; Zebrafish