title: Abnormal expression of FLOT1 correlates with tumor progression and poor survival in patients with non-small cell lung cancer.
abstract: Recent studies have revealed that flotillin-1 (FLOT1) plays important roles in cancer progression. However, the role of FLOT1 in development and progression of non-small cell lung cancer (NSCLC) remains largely unknown. The objective of the current study was to investigate the expression pattern and clinicopathological significance of FLOT1 in patients with NSCLC. Real-time quantitative polymerase chain reaction was applied to examine FLOT1 mRNA expression in 52 pairs of NSCLC tissues and adjacent noncancerous tissues. Immunohistochemistry was performed to examine FLOT1 protein expression in paraffin-embedded tissues from 106 NSCLC patients. Statistical analyses were applied to evaluate the diagnostic value and associations of FLOT1 expression with clinicopathological characteristics. FLOT1 mRNA expression was evidently upregulated in NSCLC tissues compared with that in the adjacent noncancerous tissues. In the 106 cases of tested NSCLC samples, FLOT1 protein level was positively correlated with tumor size, tumor stage, and lymph node metastasis. Patients with higher FLOT1 expression had shorter overall survival time, whereas those with lower FLOT1 expression had longer survival time. Taken together, our findings indicate that FLOT1 may play an important role in NSCLC tumorigenesis. Further elucidation of the molecular mechanisms underlying the role of FLOT1 is warranted.
authors.affiliation: Department of Thoracic Surgery, Shandong Cancer Hospital and Institute, No. 440, Jiyan Rd., Jinan, 250117, China, lihuidoc112@126.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Rui-Ming
authors.initials: RM
authors.lastname: Wang
authors.forename: Shu-Guang
authors.initials: SG
authors.lastname: Liu
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Zhang
authors.forename: Jing-Yu
authors.initials: JY
authors.lastname: Luo
authors.forename: Bai-Jiang
authors.initials: BJ
authors.lastname: Zhang
authors.forename: Xing-Guo
authors.initials: XG
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000209:etiology / Q000401:mortality* / Q000473:pathology; D018450:Disease Progression; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000209:etiology / Q000401:mortality* / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D008875:Middle Aged
chemical_list: D008565:Membrane Proteins; C106354:flotillins