title: Differences between healthy hematopoietic progenitors and leukemia cells with respect to CD44 mediated rolling versus adherence behavior on hyaluronic acid coated surfaces.
abstract: We previously demonstrated that leukemia cell lines expressing CD44 and hematopoietic progenitor cells (HPC) from umbilical cord blood (CB) showed rolling on hyaluronic acid (HA)-coated surfaces under physiological shear stress. In the present study, we quantitatively assessed the interaction of HPC derived from CB, mobilized peripheral blood (mPB) and bone marrow (BM) from healthy donors, as well as primary leukemia blasts from PB and BM of patients with acute myeloid leukemia (AML) with HA. We have demonstrated that HPC derived from healthy donors showed relative homogeneous rolling and adhesion to HA. In contrast, highly diverse behavioral patterns were found for leukemia blasts under identical conditions. The monoclonal CD44 antibody (clone BU52) abrogated the shear stress-induced rolling of HPC and leukemia blasts, confirming the significance of CD44 in this context. On the other hand, the immobile adhesion of leukemia blasts to the HA-coated surface was, in some cases, not or incompletely inhibited by BU52. The latter property was associated with non-responsiveness to induction chemotherapy and subsequently poor clinical outcome.
authors.affiliation: Institute of Functional Interfaces, Karlsruhe Institute of Technology, Karlsruhe, Germany; Applied Physical Chemistry, Heidelberg University, Heidelberg, Germany; Analytical Chemistry - Biointerfaces, Ruhr-University Bochum, Bochum, Germany; Department of Medicine V, Heidelberg University, Heidelberg, Germany.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Hanke
authors.affiliation: Department of Medicine V, Heidelberg University, Heidelberg, Germany.
authors.forename: Isabel
authors.initials: I
authors.lastname: Hoffmann
authors.affiliation: Institute of Functional Interfaces, Karlsruhe Institute of Technology, Karlsruhe, Germany; Applied Physical Chemistry, Heidelberg University, Heidelberg, Germany.
authors.forename: Christof
authors.initials: C
authors.lastname: Christophis
authors.affiliation: Department of Medicine V, Heidelberg University, Heidelberg, Germany.
authors.forename: Mario
authors.initials: M
authors.lastname: Schubert
authors.affiliation: Department of Medicine V, Heidelberg University, Heidelberg, Germany.
authors.forename: Van T
authors.initials: VT
authors.lastname: Hoang
authors.affiliation: Department of Medicine V, Heidelberg University, Heidelberg, Germany; Child and Youth Cancer Research Institute, University Center for Health Sciences, University of Guadalajara, Guadalajara, Jalisco, Mexico.
authors.forename: Abraham
authors.initials: A
authors.lastname: Zepeda-Moreno
authors.affiliation: Department of Medicine V, Heidelberg University, Heidelberg, Germany.
authors.forename: Natalia
authors.initials: N
authors.lastname: Baran
authors.affiliation: Department of Medicine V, Heidelberg University, Heidelberg, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Eckstein
authors.affiliation: Department of Medicine V, Heidelberg University, Heidelberg, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Wuchter
authors.affiliation: Institute of Functional Interfaces, Karlsruhe Institute of Technology, Karlsruhe, Germany; Analytical Chemistry - Biointerfaces, Ruhr-University Bochum, Bochum, Germany. Electronic address: axel.rosenhahn@rub.de.
authors.forename: Axel
authors.initials: A
authors.lastname: Rosenhahn
authors.affiliation: Department of Medicine V, Heidelberg University, Heidelberg, Germany. Electronic address: anthony_dick.ho@urz.uni-heidelberg.de.
authors.forename: Anthony D
authors.initials: AD
authors.lastname: Ho
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D016022:Case-Control Studies; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D018960:Hyaluronan Receptors / Q000276:immunology*; D006820:Hyaluronic Acid / Q000378:metabolism*; D007938:Leukemia / Q000473:pathology*; D036904:Leukocyte Rolling; D044085:Microfluidics; D013499:Surface Properties
chemical_list: D000911:Antibodies, Monoclonal; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid
keywords: Acute myeloid leukemia; CD44; Hematopoietic progenitor cell; Hyaluronic acid; Rolling; Shear stress