title: Chromosomal localization of structural genes and regulators in wheat by 2D electrophoresis of ditelosomic lines.
abstract: Among the 782 spots observed in two-dimensional gel electrophoresis of denatured proteins from etiolated wheat shoots, 185 were found to be variable between the euploid and 26 ditelosomic lines of 'Chinese Spring'. Thirty-five structural genes were located on 17 chromosome arms. Numerous intensity changes showing alterations in protein levels were observed and led to the following statements: 1) regulators are frequently found and can be assigned for a same polypeptide to various chromosome arms; 2) for most polypeptides homoeologous arms do not manifest similar effects; 3) nevertheless, when affecting the same polypeptide, homoeologous arms display in most cases identical regulatory effects; 4) gene dosage compensation is observed in only one out of four homoeoallelic situations.
authors.affiliation: Laboratoire de Génétique des Systèmes Végétaux, G.I.S. Moulon, F-91190, Gif-sur-Yvette, France.
authors.forename: C
authors.initials: C
authors.lastname: Colas des Francs
authors.forename: H
authors.initials: H
authors.lastname: Thiellement