title: Somatic embryogenesis from cultured leaf segments of Zea mays.
abstract: Basal leaf segments of 3 to 4 week old maize (Zea mays L.) seedlings plated on SH medium with 30 μM dicamba produced embryogenic callus and/or somatic embryos. Histological evidence showed that some of the embryos arose directly from the explant. When leaf segments with embryos were transferred to MS medium with 1.0 μM NAA, 1.0 μM IAA, 2.0 μM 2iP, and 60 g/l sucrose, the embryos germinated and the resulting seedlings could be established in culture tubes. These responses were obtained from three inbred lines, CHI31, S615, and S7.
authors.affiliation: Plant Breeding and Genetics Unit, FAO/IAEA Agricultural Programme, International Atomic Energy Agency, Seibersdorf Laboratory, P.O. Box 100, A-1400, Vienna, Austria.
authors.forename: B V
authors.initials: BV
authors.lastname: Conger
authors.forename: F J
authors.initials: FJ
authors.lastname: Novak
authors.forename: R
authors.initials: R
authors.lastname: Afza
authors.forename: K
authors.initials: K
authors.lastname: Erdelsky