title: Metabolite fluxes across the inner membrane of plant mitochondria - inhibition by phthalonic acid.
abstract: Transport and oxidation-reduction of citrate, 2-oxoglutarate and oxaloacetate by mitochondria isolated from thermogenic (Arum maculatum, Sauromatum guttatum spadices), green leaf (Pisum sativum) or etiolated (Phaseolus aureus, Helianthus tuberosus) plant tissues was found to be inhibited by phthalonic acid. No inhibition was found for NADH oxidation, glutamate, succinate or glycine transport and oxidation and malate transport. The much greater sensitivity of citrate oxidation to phthalonate inhibition compared with that of 2-oxoglutarate indicated that different carriers were involved, neither of which appeared to be rate-limiting for oxidation. Fluxes of oxaloacetate, and their sensitivity to phthalonate, indicated that this keto acid may use either the same carrier as 2-oxoglutarate or an oxaloacetate-specific carrier.
authors.affiliation: Department of Biochemistry, School of Biological Sciences, University of Sussex, Falmer, BN1 9QG, Brighton, UK.
authors.forename: M O
authors.initials: MO
authors.lastname: Proudlove
authors.forename: A L
authors.initials: AL
authors.lastname: Moore