title: Cascade reactions in multicompartmentalized polymersomes.
abstract: Enzyme-filled polystyrene-b-poly(3-(isocyano-L-alanyl-aminoethyl)thiophene) (PS-b-PIAT) nanoreactors are encapsulated together with free enzymes and substrates in a larger polybutadiene-b-poly(ethylene oxide) (PB-b-PEO) polymersome, forming a multicompartmentalized structure, which shows structural resemblance to the cell and its organelles. An original cofactor-dependent three-enzyme cascade reaction is performed, using either compatible or incompatible enzymes, which takes place across multiple compartments.
authors.affiliation: Radboud University Nijmegen, Institute for Molecules and Materials, Bio-organic Chemistry, Heyendaalseweg 135, 6525 AJ, Nijmegen (The Netherlands).
authors.forename: Ruud J R W
authors.initials: RJ
authors.lastname: Peters
authors.forename: Maïté
authors.initials: M
authors.lastname: Marguet
authors.forename: Sébastien
authors.initials: S
authors.lastname: Marais
authors.forename: Marco W
authors.initials: MW
authors.lastname: Fraaije
authors.forename: Jan C M
authors.initials: JC
authors.lastname: van Hest
authors.forename: Sébastien
authors.initials: S
authors.lastname: Lecommandoux
mesh_terms: D002384:Catalysis; D011108:Polymers / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*
chemical_list: D011108:Polymers; D011137:Polystyrenes
keywords: artificial cells; cascade reactions; enzyme catalysis; nanoreactors; polymersomes