title: S-adenosyl-L-methionine: (S)-scoulerine 9-O-methyltransferase, a highly stereo- and regio-specific enzyme in tetrahydroprotoberberine biosynthesis.
abstract: Suspension cultures of Berberis species are useful sources for the detection and isolation of a new enzyme which transfers the methyl group from S-adenosyl-L-methionine specifically to the 9-position of the (S)-enantiomer of scoulerine, producing (S)-tetrahydrocolumbamine. The enzyme was enriched 27-fold; it is not particle bound, has a pH optimum of 8.9, a molecular weight of 63 000 and shows a high degree of substrate specificity.
authors.affiliation: Lehrstuhl für Pharmazeutische Biologie der Universität München, Karlstrasse 29, D-8000, München 2, FRG.
authors.forename: S
authors.initials: S
authors.lastname: Muemmler
authors.forename: M
authors.initials: M
authors.lastname: Rueffer
authors.forename: N
authors.initials: N
authors.lastname: Nagakura
authors.forename: M H
authors.initials: MH
authors.lastname: Zenk