title: Phreatobacter oligotrophus gen. nov., sp. nov., an alphaproteobacterium isolated from ultrapure water of the water purification system of a power plant.
abstract: Strains of a novel alphaproteobacterium were isolated from ultrapure water of a Hungarian power plant on a newly developed medium. Phylogenetic analysis of the 16S rRNA gene sequences of the novel strains showed that these bacteria belong to a distinct lineage far from any known taxa. Based on the 16S rRNA gene sequences, strains PI_31, PI_25 and PI_21(T) exhibited the highest sequence similarity to Bosea minatitlanensis AMX51(T) (93.43 %) and Bosea thiooxidans DSM 9653(T) (93.36 %); similarity to all other taxa was less than 93.23 %. Fatty acid profiles, matrix-assisted laser-desorption/ionization time-of-flight mass spectra of cell extracts as well as physiological and biochemical characteristics indicated that our strains represent a novel genus and species within the class Alphaproteobacteria. The major isoprenoid quinone of the strains was Q-10, the major cellular fatty acids were C18 : 1ω7c and 11-methyl C18 : 1ω7c and the polar lipid profiles of the strains contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and several unknown phospholipids and other lipids. The characteristic diamino acid in their cell wall was meso-diaminopimelic acid. The G+C content of DNA of the proposed type strain PI_21(T) was 68.9 mol%. A new genus and species, Phreatobacter oligotrophus gen. nov., sp. nov., is proposed to accommodate the strains. Strain PI_21(T) ( = DSM 25521(T) = NCAIM B 02510(T)) is the type strain of Phreatobacter oligotrophus.
authors.affiliation: Eötvös Loránd University, Faculty of Science, Department of Microbiology, Pázmány Péter sétány 1/C, H-1117 Budapest, Hungary.
authors.forename: E M
authors.initials: EM
authors.lastname: Tóth
authors.affiliation: Eötvös Loránd University, Faculty of Science, Department of Microbiology, Pázmány Péter sétány 1/C, H-1117 Budapest, Hungary.
authors.forename: A
authors.initials: A
authors.lastname: Vengring
authors.affiliation: Eötvös Loránd University, Faculty of Science, Department of Microbiology, Pázmány Péter sétány 1/C, H-1117 Budapest, Hungary.
authors.forename: Z G
authors.initials: ZG
authors.lastname: Homonnay
authors.affiliation: Eötvös Loránd University, Faculty of Science, Department of Microbiology, Pázmány Péter sétány 1/C, H-1117 Budapest, Hungary.
authors.forename: Zs
authors.initials: Z
authors.lastname: Kéki
authors.affiliation: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, D-38124 Braunschweig, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Spröer
authors.affiliation: Eötvös Loránd University, Faculty of Science, Department of Microbiology, Pázmány Péter sétány 1/C, H-1117 Budapest, Hungary.
authors.forename: A K
authors.initials: AK
authors.lastname: Borsodi
authors.affiliation: Eötvös Loránd University, Faculty of Science, Department of Microbiology, Pázmány Péter sétány 1/C, H-1117 Budapest, Hungary.
authors.forename: K
authors.initials: K
authors.lastname: Márialigeti
authors.affiliation: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, D-38124 Braunschweig, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Schumann
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D006814:Hungary; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D011210:Power Plants; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry; D014871:Water Microbiology; D018508:Water Purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D003960:Diaminopimelic Acid; C025204:Ubiquinone Q2