title: α-Ketoacids as precursors for phenylalanine and tyrosine labelling in cell-based protein overexpression.
abstract: (13)C-α-ketoacid metabolic precursors of phenylalanine and tyrosine effectively enter the metabolism of a protein overexpressing E. coli strain to label Phe- and Tyr-residues devoid of any cross-labelling. The methodology gives access to highly selective labelling patterns as valuable tools in protein NMR spectroscopy without the need of (15)N-chiral amino acid synthesis using organic chemistry.
authors.affiliation: Institute of Organic Chemistry, University of Vienna, Währingerstr. 38, 1090, Vienna, Austria, roman.lichtenecker@univie.ac.at.
authors.forename: Roman J
authors.initials: RJ
authors.lastname: Lichtenecker
authors.forename: Katharina
authors.initials: K
authors.lastname: Weinhäupl
authors.forename: Walther
authors.initials: W
authors.lastname: Schmid
authors.forename: Robert
authors.initials: R
authors.lastname: Konrat
mesh_terms: D004926:Escherichia coli / Q000378:metabolism; D007553:Isotope Labeling; D007554:Isotopes / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D007651:Keto Acids / Q000737:chemistry* / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010649:Phenylalanine / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism; D014443:Tyrosine / Q000737:chemistry / Q000378:metabolism*
chemical_list: D007554:Isotopes; D007651:Keto Acids; D011994:Recombinant Proteins; D014443:Tyrosine; D010649:Phenylalanine