title: Nuclear DNA content and the control of chloroplast replication in wheat leaves.
abstract: During development of the first leaf of breadwheat (Triticum aestivum L.) the number of chloroplasts per mesophyll cell increases between three- and four-fold. To establish if chloroplast replication is accompanied by endoreduplication, the nuclear DNA content of the cells was determined by chemical assay of isolated nuclei from mesophyll protoplasts and by microdensitometry of nuclei in mesophyll tissue. The DNA content of the nuclei was constant (27 to 32 pg) at each phase of chloroplast replication. Approximately 93% of the cells had a nuclear DNA content close to the 2C value of 32 pg. It is concluded that chloroplast replication is not dependent on nuclear endoreduplication in seedling leaves of wheat.
authors.affiliation: Department of Biology, University of York, Heslington, YO1 5DD, York, UK.
authors.forename: J R
authors.initials: JR
authors.lastname: Ellis
authors.forename: A J
authors.initials: AJ
authors.lastname: Jellings
authors.forename: R M
authors.initials: RM
authors.lastname: Leech