title: Assessment of chromium-contaminated groundwater using a thiosulfate-oxidizing bacteria (TOB) biosensor.
abstract: The effect of Cr(6+)-contaminated groundwater was assessed using thiosulfate-oxidizing bacteria (TOB). Electrical conductivity (EC), pH, and sulfate production were determined based on thiosulfate oxidation. Final pH values in the different test treatments of Cr(6+)-contaminated groundwater (50-1000 μg Cr(6+)L(-1)) ranged from 2.02 ± 0.09 to 7.76 ± 0.07 and EC ranged from 5.95 ± 0.03 to 3.63 ± 0.03 mS cm(-1). Inhibition of TOB due to Cr(6+) was between 16.7% and 100%, with higher levels of inhibition occurring at higher Cr(6+) concentrations. The median effective concentration (EC50) was 78.96 μg Cr(6+)L(-1). These data demonstrate that TOB can detect less than 100 μg L(-1) of Cr(6+) in the groundwater and can be used as an effective bioassay for toxicity assessment.
authors.affiliation: Department of Biological Environment, Kangwon National University, Gangwon-do, Chuncheon-si, South Korea.
authors.forename: Naveed Ahmed
authors.initials: NA
authors.lastname: Qambrani
authors.affiliation: Department of Biosystems Engineering, Kangwon National University, Gangwon-do Chuncheon-si, South Korea.
authors.forename: Beom-Soo
authors.initials: BS
authors.lastname: Shin
authors.affiliation: Department of Bio-environmental Sciences, Sunchon National University, Suncheon 540-950, Korea. Electronic address: chojs@sunchon.ac.kr.
authors.forename: Ju-Sik
authors.initials: JS
authors.lastname: Cho
authors.affiliation: Department of Biological Environment, Kangwon National University, Gangwon-do, Chuncheon-si, South Korea. Electronic address: ohsangeun@kangwon.ac.kr.
authors.forename: Sang-Eun
authors.initials: SE
authors.lastname: Oh
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D002857:Chromium / Q000032:analysis*; D004553:Electric Conductivity; D060587:Groundwater / Q000032:analysis*; D057230:Limit of Detection; D010084:Oxidation-Reduction; D013885:Thiosulfates / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D013885:Thiosulfates; D014874:Water Pollutants, Chemical; D002857:Chromium
keywords: Cr(6+) toxicity in groundwater; Heavy metal; Sulfur oxidation; Thiosulfate-oxidizing bacteria (TOB); Toxicity assessment