title: Anti-sense RNAs of cucumber mosaic virus in transgenic plants assessed for control of the virus.
abstract: Three synthetic genes for the production of anti-sense RNA to different regions of the cucumber mosaic virus (CMV) genome were constructed using virus-derived double-stranded cDNA coupled to a promoter sequence from cauliflower mosaic virus. The genes were used to transform tobacco plants by a Ti plasmid vector. Transgenic plants obtained with the three constructs produced anti-sense RNA at different levels. Plants expressing each of the three anti-sense RNAs were inoculated with CMV and their sensitivity to the virus infection was compared with the non-transformed plants. Only one plant line which expressed relatively low levels of one of the anti-sense RNAs showed resistance to CMV but other plants expressing the same or the other two antisense RNAs had similar sensitivity to CMV infection as the non-transformed plants.
authors.affiliation: CSIRO Division of Horticulture, P.O. Box 350, 5001, Adelaide, S.A., Australia.
authors.forename: M A
authors.initials: MA
authors.lastname: Rezaian
authors.forename: K G
authors.initials: KG
authors.lastname: Skene
authors.forename: J G
authors.initials: JG
authors.lastname: Ellis