title: Utilization of nitrate, nitrite and ammonium by Chlamydomonas reinhardii : Photoproduction of ammonium.
abstract: In phototrophically grown Chlamydomonas cells, ammonium strongly inhibited the utilization of nitrate or nitrite. Under darkness, or in the presence of an uncoupler or inhibitor of the non-cyclic photosynthetic electron flow, the utilization of nitrate, nitrite or ammonium was suppressed. L-Methionine-D,L-sulfoximine (MSX) or azaserine, which blocks the assimilation of ammonium, inhibited the consumption of nitrate, but not nitrite, by the cells. Ammonium produced an immediate inhibition of the permease for nitrate in Chlamydomonas growing with nitrate, while ammonium-grown cells lacked this permease. The synthesis of nitrate-reductase activity was dependent on an active permease. In N-starved Chlamydomonas cells, previously treated with MSX, the permease for nitrate was insensitive to inhibition by ammonium, and a significant amount of nitrate reductase was synthetized. These cells photoproduce ammonium by reducing nitrate. Nitrogen-repleted cells, treated with MSX, actively photoproduced ammonium by reducing nitrite, but not nitrate.
authors.affiliation: Departamento de Bioquímica, Facultad de Biología, Universidad de Sevilla y C.S.I.C. Sevilla, Sevilla, Spain.
authors.forename: F J
authors.initials: FJ
authors.lastname: Florencio
authors.forename: J M
authors.initials: JM
authors.lastname: Vega