title: Superoxide dismutase in Scenedesmus obliquus : Effect of growth conditions and initial characterization.
abstract: In heterotrophically grown Scenedesmus obliquus, the specific activity of superoxide dismutase (SOD; EC 1.15.1.1) declined when glucose was abundant, increased as it was depleated, and remained steady at a high level when it was absent. Transition to autotrophic growth produced only a small (20% over 5 d) increase in specific activity above the values obtained in dark-grown cells after glucose and starch-reserve depletion. This small, but consistent, increase did, however, parallel a similar increase in photosynthetic capacity. Polyacrylamide-gel electrophoresis showed the existence of nine isoenzymes of SOD. The three major and one of the minor isoenzymes were present in all extracts while three minor isoenzymes were found only in autotrophically grown cells and two only in heterotrophically grown cells. Characterization studies indicated that two of the major isoenzymes are dimeric FeSODs the other is a tetrameric MnSOD, and of the minor isoenzymes, two are dimeric FeSODs and four are dimeric MnSODs.
authors.affiliation: Department of Biochemistry, University of Liverpool, P.O. Box 147, L69 3BX, Liverpool, UK.
authors.forename: M J
authors.initials: MJ
authors.lastname: Redmond
authors.forename: A R
authors.initials: AR
authors.lastname: McEuen
authors.forename: R
authors.initials: R
authors.lastname: Powls