title: Blocked autophagy enhances radiosensitivity of nasopharyngeal carcinoma cell line CNE-2 in vitro.
abstract: CONCLUSIONS
  Autophagy was involved in the radiation treatment of CNE-2 cells, and blocked autophagy enhances the radiosensitivity of nasopharyngeal carcinoma cell line CNE-2 in vitro.
  
  
  OBJECTIVE
  To determine whether autophagy induced by radiation therapy contributes to tumor cell death or represents a mechanism of resistance to therapy-mediated cell death.
  
  
  METHODS
  Autophagy in the CNE-2 nasopharyngeal carcinoma cells after radiation treatment was determined by quantitative GFP-LC3 analysis, electron microscopy, and autophagy-related molecules analysis by Western blotting. The contribution of autophagy to the cell viability was determined by MTT assay and clonogenic assay.
  
  
  RESULTS
  Autophagy was involved in CNE-2 cells post radiation treatment, and autophagy could ameliorate the cell viability post radiation. On the other hand, inhibition of autophagy could reduce cell viability and decrease the cell survival.
authors.affiliation: Department of Otolaryngology Head & Neck Surgery, First Hospital of Jilin University , Changchun , China.
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Wang
authors.forename: Wanzhong
authors.initials: W
authors.lastname: Yin
authors.forename: Xuewei
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives; D001343:Autophagy; D002277:Carcinoma; D045744:Cell Line, Tumor; D002470:Cell Survival; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism / Q000503:physiopathology / Q000532:radiotherapy*; D011836:Radiation Tolerance
chemical_list: C025946:3-methyladenine; D000225:Adenine