title: A novel fluorescence assay for the detection of hemoglobin based on the G-quadruplex/hemin complex.
abstract: A novel sensing strategy for specific recognition of hemoglobin has been designed. In the presence of trypsin, hemoglobin was cleaved to release heme which could effectively bind to a FITC-labeled guanine (G)-rich oligonucleotide sequence to form the G-quadruplex/hemin complex. This binding process would lead to fluorescence quenching of FITC via photoinduced electron transfer. Therefore, a fluorescence assay for the determination of hemoglobin could be established. Under the optimized conditions, there was a linear relationship between the fluorescence intensity of probe DNA and the hemoglobin concentration in the range of 5-200 nmol/L, with the limit of detection (LOD) as low as 2 nmol/L.
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Pang
authors.forename: Siyu
authors.initials: S
authors.lastname: Liu
authors.forename: Xingguang
authors.initials: X
authors.lastname: Su
mesh_terms: D000818:Animals; D001681:Biological Assay; D015374:Biosensing Techniques; D002417:Cattle; D004912:Erythrocytes / Q000378:metabolism*; D005453:Fluorescence; D054856:G-Quadruplexes; D006147:Guanine / Q000737:chemistry*; D006427:Hemin / Q000737:chemistry* / Q000378:metabolism; D006454:Hemoglobins / Q000032:analysis*; D057230:Limit of Detection; D013050:Spectrometry, Fluorescence
chemical_list: D006454:Hemoglobins; D006147:Guanine; D006427:Hemin
keywords: Fluorescence quenching; G-quadruplex/hemin complex; Hemoglobin; Trypsin