title: Short-term dynamics of culturable bacteria in a soil amended with biotransformed dry olive residue.
abstract: Dry olive residue (DOR) transformation by wood decomposing basidiomycetes (e.g. Coriolopsis floccosa) is a possible strategy for eliminating the liabilities related to the use of olive oil industry waste as an organic soil amendment. The effects of organic fertilization with DOR on the culturable soil microbiota are largely unknown. Therefore, the objectives of this study were to measure the short-term effects of DOR and C. floccosa-transformed DOR on the culturable bacterial soil community, while at the same time documenting the bacterial diversity of an agronomic soil in the southeastern Iberian Peninsula. The control soil was compared with the same soil treated with DOR and with C. floccosa-transformed DOR for 0, 30 and 60 days. Impact was measured from total viable cells and CFU counts, as well as the isolation and characterization of 900 strains by fatty acid methyl ester profiles and 16S rRNA partial sequencing. The bacterial diversity was distributed between Actinobacteria, Alphaproteobacteria, Gammaproteobacteria, Betaproteobacteria, Bacilli, Sphingobacteria and Cytophagia. Analysis of the treatments and controls demonstrated that soil amendment with untransformed DOR produced important changes in bacterial density and diversity. However, when C. floccosa-transformed DOR was applied, bacterial proliferation was observed but bacterial diversity was less affected, and the distribution of microorganisms was more similar to the unamended soil.
authors.affiliation: Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), C/Profesor Albareda 1, E-18008 Granada, Spain. Electronic address: jose.siles@eez.csic.es.
authors.forename: J A
authors.initials: JA
authors.lastname: Siles
authors.affiliation: Department of Microbiology, Fundación MEDINA, Parque Tecnológico de Ciencias de la Salud, Avd. del Conocimiento, 3, E-18016 Granada, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Pascual
authors.affiliation: Department of Microbiology, Fundación MEDINA, Parque Tecnológico de Ciencias de la Salud, Avd. del Conocimiento, 3, E-18016 Granada, Spain.
authors.forename: V
authors.initials: V
authors.lastname: González-Menéndez
authors.affiliation: Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), C/Profesor Albareda 1, E-18008 Granada, Spain; College of Engineering and Mathematical Sciences, University of Vermont, 203B Perkins, 33 Colchester Avenue, Burlington, VT 05405, USA.
authors.forename: I
authors.initials: I
authors.lastname: Sampedro
authors.affiliation: Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), C/Profesor Albareda 1, E-18008 Granada, Spain.
authors.forename: I
authors.initials: I
authors.lastname: García-Romera
authors.affiliation: Department of Microbiology, Fundación MEDINA, Parque Tecnológico de Ciencias de la Salud, Avd. del Conocimiento, 3, E-18016 Granada, Spain; Texas Therapeutics Institute, The Brown Foundation Institute for Molecular Medicine, University of Texas Health Science Center at Houston, Houston, TX 77054, USA.
authors.forename: G F
authors.initials: GF
authors.lastname: Bills
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000254:growth & development; D058491:Bacterial Load; D058448:Biota / Q000187:drug effects*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D050296:Microbial Viability; D008969:Molecular Sequence Data; D031658:Olea / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013030:Spain
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: Bioremediation; Culturable bacteria; Dry olive residue; Granada soil; Organic amendments and fertilizers; Soil bacteria