title: Permeabilization of plant cells: (31)P NMR studies on the permeability of the tonoplast.
abstract: A suspension culture of Catharanthus roseus has been used to study the permeability of cell membranes after treatment with various concentrations of a permeabilizing agent (DMSO). The uptake and release (after permeabilization) of inorganic phosphate (Pi) by cells have been investigated by (32)P radiotracer and non-invasive phosphorus-31 NMR experiments. These studies have demonstrated that measurements of the Pi-efflux from plant cells provide a reliable measure of the permeability of the tonoplast.
authors.affiliation: Department of Physical Chemistry 2, University of Lund, Chemical Center, P.O. Box 124, S-22100, Lund, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Lundberg
authors.forename: L
authors.initials: L
authors.lastname: Linsefors
authors.forename: H J
authors.initials: HJ
authors.lastname: Vogel
authors.forename: P
authors.initials: P
authors.lastname: Brodelius