title: Analysis of Y chromosome haplogroups in Japanese population using short amplicons and its application in forensic analysis.
abstract: We designed three mini multiplex PCR systems using single-base extension reactions to identify Japanese Y chromosome haplogroups. We selected a group of 22 Y chromosome single nucleotide polymorphisms (SNPs) from the haplogroups most commonly reported in East Asia. To make the systems more useful in analyzing degraded DNA samples, we designed primers to render amplicons of ≤ 150 bp. Applying these systems, we classified the Japanese population into major haplogroups and confirmed the applicability of these systems in forensic DNA analysis.
authors.affiliation: Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan. Electronic address: 10mh027c@shinshu-u.ac.jp.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Harayama
authors.affiliation: Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Sayako
authors.initials: S
authors.lastname: Kamei
authors.affiliation: Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Sato
authors.affiliation: Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Tokutaro
authors.initials: T
authors.lastname: Hayashi
authors.affiliation: Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Shiozaki
authors.affiliation: Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Ota
authors.affiliation: Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Asamura
mesh_terms: D044466:Asian People / Q000235:genetics*; D041322:Chromosomes, Human, Y; D053803:Forensic Genetics / Q000379:methods*; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D008297:Male; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
keywords: Degraded DNA; Multiplex single base extension reactions; Short PCR products; Y chromosome SNPs