title: Detection of Legionella spp. and Legionella pneumophila in water samples of Spain by specific real-time PCR.
abstract: Legionella pneumophila is the primary cause of the legionellosis diseases (90 %) (Yu et al. in J Infect Dis 186:127-128, 2002; Doleans et al. in J Clin Microbiol 42:458-460, 2004; Den Boer et al. in Clin Microbiol Infect 14:459-466, 2008). In this study, methodologies based on molecular biology were developed in order to provide a quick diagnosis of the bacterial presence in water samples of Spain. Multiplex real-time polymerase chain reaction assays were realized to target the 16S rRNA and macrophage infectivity potentiator (mip) genes of, respectively, Legionella spp. and L. pneumophila including in the design of an internal control. The results obtained by the culture and the gene amplification methods agreed in 94.44 % for the 16S rRNA gene, and a concordance of 66.67 % of the cases was obtained for the mip gene.
authors.affiliation: Laboratory of Cytogenetics and Molecular Genetics, Faculty of Veterinary, University of Zaragoza, 177 Miguel Servet, 50013, Zaragoza, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Grúas
authors.forename: Silvia
authors.initials: S
authors.lastname: Llambi
authors.forename: M Victoria
authors.initials: MV
authors.lastname: Arruga
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D004784:Environmental Monitoring / Q000379:methods*; D006801:Humans; D007875:Legionella / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D016952:Legionella pneumophila / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D019696:Peptidylprolyl Isomerase / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013030:Spain; D014871:Water Microbiology
chemical_list: D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; C512922:Mip protein, Legionella pneumophila; D019696:Peptidylprolyl Isomerase