title: The role of nickel in urea assimilation by algae.
abstract: Nickel is required for urease synthesis by Phaeodactylum tricornutum and Tetraselmis subcordiformis and for growth on urea by Phaeodactylum. There is no requirement for nickel for urea amidolyase synthesis by Chlorella fusca var. vacuolata. Neither copper nor palladium can substitute for nickel but cobalt partially restored urease activity in Phaeodactylum. The addition of nickel to nickel-deficient cultures of Phaeodactylum or Tetraselmis resulted in a rapid increase of urease activity to 7-30 times the normal level; this increase was not inhibited by cycloheximide. It is concluded that nickel-deficient cells over-produce a non-functional urease protein and that either nickel or the functional urease enzyme participates in the regulation of the production of urease protein.
authors.affiliation: Department of Botany and Microbiology, University College Swansea, Singleton Park, SA2 8PP, Swansea, UK.
authors.forename: T A
authors.initials: TA
authors.lastname: Rees
authors.forename: I A
authors.initials: IA
authors.lastname: Bekheet