title: Investigation of the in vivo antioxidative activity of Cynara scolymus (artichoke) leaf extract in the streptozotocin-induced diabetic rat.
abstract: The in vivo antioxidant activity of a quantified leaf extract of Cynara scolymus (artichoke) was studied. The aqueous artichoke leaf extract (ALE), containing 1.5% caffeoylquinic acid with chlorogenic acid being most abundant (0.30%), and luteolin-7-O-glucoside as major flavonoid (0.15%), was investigated by evaluating the effect on different oxidative stress biomarkers, after 3 wk oral supplementation in the streptozotocin-induced diabetic rat model. Apart from two test groups (0.2 g ALE/kg BW/day and 1 g ALE/kg BW/day, where BW is body weight), a healthy control group, untreated oxidative stress group, and vitamin E treated group (positive control) were included. A 0.2 g/kg BW/day of ALE decreased oxidative stress: malondialdehyde and 8-hydroxydeoxyguanosine levels significantly diminished, whereas erythrocyte glutathione levels significantly increased. A 1.0 g/kg BW/day ALE did not show higher antioxidant activity.
authors.affiliation: NatuRA (Natural products and Food Research and Analysis)-Laboratory of Nutrition and Functional Food Science, Department of Pharmaceutical Sciences, University of Antwerp, Antwerpen, Belgium.
authors.forename: Joanna
authors.initials: J
authors.lastname: Magielse
authors.forename: Annelies
authors.initials: A
authors.lastname: Verlaet
authors.forename: Annelies
authors.initials: A
authors.lastname: Breynaert
authors.forename: Begoña Manuel Y
authors.initials: BM
authors.lastname: Keenoy
authors.forename: Sandra
authors.initials: S
authors.lastname: Apers
authors.forename: Luc
authors.initials: L
authors.lastname: Pieters
authors.forename: Nina
authors.initials: N
authors.lastname: Hermans
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D031943:Cynara scolymus / Q000737:chemistry*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism; D019587:Dietary Supplements; D005978:Glutathione / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D008315:Malondialdehyde; D013311:Streptozocin; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D005978:Glutathione
keywords: 8-Hydroxydeoxyguanosine (8-OHdG); Artichoke; Cynara scolymus; Glutathione (GSH); Malondialdehyde (MDA)