title: Identification of methanogenic archaea in the hyporheic sediment of Sitka stream.
abstract: Methanogenic archaea produce methane as a metabolic product under anoxic conditions and they play a crucial role in the global methane cycle. In this study molecular diversity of methanogenic archaea in the hyporheic sediment of the lowland stream Sitka (Olomouc, Czech Republic) was analyzed by PCR amplification, cloning and sequencing analysis of the methyl coenzyme M reductase alpha subunit (mcrA) gene. Sequencing analysis of 60 clones revealed 24 different mcrA phylotypes from hyporheic sedimentary layers to a depth of 50 cm. Phylotypes were affiliated with Methanomicrobiales, Methanosarcinales and Methanobacteriales orders. Only one phylotype remains unclassified. The majority of the phylotypes showed higher affiliation with uncultured methanogens than with known methanogenic species. The presence of relatively rich assemblage of methanogenic archaea confirmed that methanogens may be an important component of hyporheic microbial communities and may affect CH4 cycling in rivers.
authors.affiliation: Department of Ecology and Environmental Sciences - Laboratory of Aquatic Microbial Ecology, Faculty of Science, Palacky University, Olomouc, Olomouc, Czech Republic.
authors.forename: Iva
authors.initials: I
authors.lastname: Buriánková
authors.forename: Lenka
authors.initials: L
authors.lastname: Brablcová
authors.forename: Václav
authors.initials: V
authors.lastname: Mach
authors.forename: Petr
authors.initials: P
authors.lastname: Dvořák
authors.forename: Prem Prashant
authors.initials: PP
authors.lastname: Chaudhary
authors.forename: Martin
authors.initials: M
authors.lastname: Rulík
mesh_terms: D001105:Archaea / Q000235:genetics*; D018153:Czech Republic; D004783:Environmental Microbiology; D015723:Gene Library; D019766:Genes, Archaeal; D019015:Geologic Sediments / Q000382:microbiology*; D008697:Methane / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D045483:Rivers / Q000382:microbiology*
chemical_list: D008697:Methane