title: Sensitive determination of four tetracycline antibiotics in pig plasma by field-amplified sample stacking open-tubular capillary electrochromatography with dimethylethanolamine aminated polychloromethyl styrene nano-latex coated capillary column.
abstract: This paper described the preparation and application of a new dimethylethanolamine aminated polychloromethyl styrene nano-latex (DMEAPL) coated capillary column (ccc-DMEAPL) in the determination of four tetracycline antibiotics (TCA) including tetracycline (TC), oxytetracycline (OTC), doxycycline (DC) and chlorotetracycline (CTC) in pig plasma. The ccc-DMEAPL column was characterized with steady EOF values of ca. 1.5-5.2×10(-5)cm(2)/Vs at pH 1.8-6.3. The optimized conditions for field-amplified sample stacking open-tubular capillary electrochromatography (FASS-OT-CEC) were as following: background electrolyte, 10mmol/L Na2HPO4+15mmol/L citric acid (pH 3.2); ccc-DMEAPL, 50μm i.d.×50cm (effective length 41.5cm), separation voltage, 18kV; column temperature, 25°C; UV detection wavelength, 270nm; water-plug injection: 30mbar×10s; sample electrokinetic injection, 10kV×20s. The four TCA were extracted with the solution of 10mmol/L Na2HPO4+15mmol/L citric acid+4g/L EDTA-2Na (pH 3.2). The FASS-OT-CEC method was validated in terms of linearity, sensitivity, selectivity, precision and accuracy. The LODs ranged from 3 to 7ng/mL, the recoveries for the four TCA were all more than 80%. The developed method was successfully applied for the determination of TCs in the actual pig plasma samples.
authors.affiliation: College of Chemistry & Molecular Engineering, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Yaxiao
authors.initials: Y
authors.lastname: Guo
authors.forename: Lei
authors.initials: L
authors.lastname: Meng
authors.forename: Yanhao
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.forename: Wenfen
authors.initials: W
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Xia
authors.forename: Fuguo
authors.initials: F
authors.lastname: Ban
authors.forename: Ningpeng
authors.initials: N
authors.lastname: Wu
authors.forename: Shusheng
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood* / Q000737:chemistry; D053801:Capillary Electrochromatography / Q000295:instrumentation* / Q000379:methods*; D003642:Deanol / Q000737:chemistry*; D007840:Latex / Q000737:chemistry; D057230:Limit of Detection; D016014:Linear Models; D053758:Nanoparticles / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D015203:Reproducibility of Results; D013552:Swine; D013754:Tetracyclines / Q000097:blood* / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D007840:Latex; D011137:Polystyrenes; D013754:Tetracyclines; D003642:Deanol
keywords: Dimethylethanolamine aminated polychloromethyl styrene; Field-amplified sample stacking; Nano-latex; Open-tubular capillary electrochromatography; Plasma; Tetracycline antibiotics