title: Fatty acid binding protein 7 as a marker of glioma stem cells.
abstract: Glioblastomas are the most aggressive brain tumors. Glioblastoma stem cells (GSCs) are thought to be responsible for the recurrence, chemoresistance, and poor prognosis of glioblastoma. Fatty acid binding protein 7 (FABP7), which is a cellular chaperone for a variety of omega-3 fatty acids, is a known marker for neural stem cells. In this study, using a newly developed anti-FABP7 antibody and patient-derived GSC lines, we evaluated the expression of FABP7 in GSCs. Using immunocytochemistry, Western blotting, and qPCR analyses, FABP7 was found to be highly enriched in GSCs and its localization was found in cytosol and nuclei. FABP7 expression was significantly downregulated in differentiated GSCs induced by the addition of serum. In the glioma surgical specimens, FABP7 was highly expressed in the majority of glioblastoma. Double immunostaining for FABP7 and Sox2 showed that FABP7(+) Sox2(+) tumor cells were significantly increased in glioblastoma (grade IV) compared with diffuse astrocytoma (grade II) and anaplastic astrocytoma (grade III). Our data introduces FABP7 as a marker for GSCs and further highlights its possible significance for glioma diagnosis and treatment.
authors.affiliation: Department of Neurosurgery, Yamaguchi University Graduate School of Medicine, Ube, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Morihiro
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yasumoto
authors.forename: Linda Koshy
authors.initials: LK
authors.lastname: Vaidyan
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Sadahiro
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Uchida
authors.forename: Akinori
authors.initials: A
authors.lastname: Inamura
authors.forename: Kazem
authors.initials: K
authors.lastname: Sharifi
authors.forename: Makoto
authors.initials: M
authors.lastname: Ideguchi
authors.forename: Sadahiro
authors.initials: S
authors.lastname: Nomura
authors.forename: Nobuko
authors.initials: N
authors.lastname: Tokuda
authors.forename: Shoji
authors.initials: S
authors.lastname: Kashiwabara
authors.forename: Aya
authors.initials: A
authors.lastname: Ishii
authors.forename: Eiji
authors.initials: E
authors.lastname: Ikeda
authors.forename: Yuji
authors.initials: Y
authors.lastname: Owada
authors.forename: Michiyasu
authors.initials: M
authors.lastname: Suzuki
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002352:Carrier Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D000071438:Fatty Acid-Binding Protein 7; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000473:pathology; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D002352:Carrier Proteins; C107608:FABP7 protein, human; D000071438:Fatty Acid-Binding Protein 7; D025521:Tumor Suppressor Proteins
keywords: FABP7; differentiation; glioblastoma; stem cells