title: Enhanced Electrostatic Discrimination of Proteins on Nanoparticle-Coated Surfaces.
abstract: Two β-lactoglobulin (BLG) isoforms, BLGA and BLGB, were used a test bed for the differentiation of proteins using electrostatics. In these studies, the BLGA and BLGB binding to a highly charged, cationic gold nanoparticle (GNP) modified surface was investigated by atomic force microscopy (AFM) and surface plasmon resonance (SPR) spectroscopy The binding affinity, and more importantly, the selectivity of this surface towards these two almost identical protein isoforms were both significantly increased on the cationic GNP surface array relative to the values measured with the same free cationic GNP in solution. While protein recognition is traditionally achieved almost exclusively via orientation dependent short-range interactions such as hydrogen bonds and hydrophobic interactions, our results show the potential of protein recognition platforms based on enhanced electrostatic interactions.
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst, MA 01003, USA ; State Key Laboratory of Chemical Engineering, East China University of Science and Technology, Shanghai 200237, P.R. China.
authors.forename: Yisheng
authors.initials: Y
authors.lastname: Xu
authors.forename: Yoni
authors.initials: Y
authors.lastname: Engel
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Yan
authors.forename: Kaimin
authors.initials: K
authors.lastname: Chen
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Moyano
authors.forename: Paul L
authors.initials: PL
authors.lastname: Dubin
authors.forename: Vincent M
authors.initials: VM
authors.lastname: Rotello