title: A selective tissue culture medium for growth of compact (dwarf) mutants of apple.
abstract: Three strains of 'McIntosh' apple (Malus domestica Borkh.) with growth habits ranging from the standard parent type to extremely compact (dwarf) were grown in vitro as meristem-tip cultures on Murashige and Skoog medium containing a range of concentrations of benzyladenine (BA). All strains exhibited a similar optima (3 to 6 μM BA) for maximum shoot proliferation and culture weight increase. However, tolerance to supra-optimal concentration of this cytokinin was related to growth habit. For example, at 10 μM BA shoot production rates as a percent of the maximum rates were 90%, 20% and zero for the extreme compact, moderate compact and standard strains, respectively. Comparisons among field trees and meristem-tip cultures of all three strains revealed similarities in growth and development.
authors.affiliation: Agriculture Canada, Summerland, B.C., Canada.
authors.forename: W D
authors.initials: WD
authors.lastname: Lane
authors.forename: N E
authors.initials: NE
authors.lastname: Looney
authors.forename: F
authors.initials: F
authors.lastname: Måge