title: Effect of miR-29b-1* and miR-29c knockdown on cell growth of the bladder cancer cell line T24.
abstract: OBJECTIVE
  To investigate the role of the microRNAs miR-29b-1-5p (miR-29b-1*) and miR-29c in bladder urothelial cancer (BUC).
  
  
  METHODS
  Levels of miR-29b-1* and miR-29c in normal urothelial cells (HU609) and BUC cells (T24) were determined via quantitative real-time reverse transcription-polymerase chain reaction. T24 cells were transfected with small interfering RNA targeting miR-29b-1* or miR-29c, and cell growth was assessed using 3-(4,5-dimehylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The predicted targets and oncogenic pathways of these microRNAs were determined using bioinformatics analysis.
  
  
  RESULTS
  MiR29b-1* and miR-29c levels were higher in T24 cells than normal urothelial cells. Knockdown of miR-29b-1* or miR-29c suppressed T24 cell growth. Bioinformatic analysis showed that miR-29b-1* and miR-29c co-regulated a subset of putative target genes, about 10% of which have been experimentally validated.
  
  
  CONCLUSIONS
  Both miR-29b-1* and miR-29c regulate cell growth in BUC. The targets of miR-29b-1* and miR-29c may be functionally associated with proliferation, cell cycle and apoptosis.
authors.affiliation: Department of Urology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.forename: Qingling
authors.initials: Q
authors.lastname: Zhang
authors.forename: Wen
authors.initials: W
authors.lastname: Cheng
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Zhang
authors.forename: Jiandong
authors.initials: J
authors.lastname: Wang
authors.forename: Jingping
authors.initials: J
authors.lastname: Ge
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering; D001749:Urinary Bladder Neoplasms / Q000235:genetics*; D019459:Urothelium / Q000166:cytology
chemical_list: C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering
keywords: Bladder urothelial cancer; cancer initiatome; miR-29b-1-5p; miR-29c; microRNA; onco-miRs