title: Secretion kinetics of hydroxyproline-containing macromolecules in carrot root discs.
abstract: Short-term pulse-chase studies using radioactive L-proline on carrot tissue support the classical endomembrane route for secretory proteins. Labelled hydroxyproline residues were first detectable in fractions containing the endoplasmic reticulum (ER) after a 5 min pulse. Upon chase-out this fraction looses and, initially, the Golgi apparatus (GA) fraction gains radioactivity. Unlike ER and GA fractions which show chase-out characteristics a plasma membrane (PM) containing-fraction reveals retention of some of the radioactivity.
authors.affiliation: Abteilung Cytologie des Pflanzenphysiologischen Instituts der Universität Göttingen, Untere Karspüle 2, D-3400, Göttingen, Germany.
authors.forename: D G
authors.initials: DG
authors.lastname: Robinson
authors.forename: R
authors.initials: R
authors.lastname: Glas