title: Overexpression of JAM-A in non-small cell lung cancer correlates with tumor progression.
abstract: The objective of the current study was to determine the clinical significance of junctional adhesion molecule A (JAM-A) in patients with non-small cell lung cancer (NSCLC) and the biological function of JAM-A in NSCLC cell lines. We showed that JAM-A is predominantly expressed in cell membranes and high expression of JAM-A occurred in 37% of lung tumor specimens compared to corresponding normal tissues. High expression of JAM-A was significantly correlated with TNM stage (P = 0.021), lymph node metastasis (P = 0.007), and decreased overall survival (P = 0.02), In addition, we observed that silencing JAM-A by small interfering RNA inhibited tumor cell proliferation and induced cell cycle arrest at the G1/S boundary. Western blotting analysis revealed that knockdown of JAM-A decreased the protein levels of cyclin D1, CDK4, 6, and P-Rb. Thus, JAM-A plays an important role in NSCLC progression.
authors.affiliation: Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang, People's Republic of China ; Department of Pathology, College of Basic Medical Sciences, Shenyang Medical College, Shenyang, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Wenting
authors.initials: W
authors.lastname: Luo
authors.forename: Bo
authors.initials: B
authors.lastname: Huang
authors.forename: Zihui
authors.initials: Z
authors.lastname: Liu
authors.forename: Limei
authors.initials: L
authors.lastname: Sun
authors.forename: Qingfu
authors.initials: Q
authors.lastname: Zhang
authors.forename: Xueshan
authors.initials: X
authors.lastname: Qiu
authors.forename: Ke
authors.initials: K
authors.lastname: Xu
authors.forename: Enhua
authors.initials: E
authors.lastname: Wang
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000172:deficiency / Q000235:genetics*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation; D018450:Disease Progression; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D011956:Receptors, Cell Surface / Q000172:deficiency / Q000235:genetics*
chemical_list: D015815:Cell Adhesion Molecules; C407243:F11R protein, human; D011956:Receptors, Cell Surface