title: Coffee treatment prevents the progression of sarcopenia in aged mice in vivo and in vitro.
abstract: Sarcopenia is characterized by the age-related loss of muscle mass and strength, which results in higher mortality in aged people. One of the mechanisms of the sarcopenia is the loss in the function and number of muscle satellite cells. Chronic low-grade inflammation plays a central role in the pathogenesis of age-related sarcopenia. Accumulating evidence suggests that coffee, one of the most widely consumed beverages in the world, has potential pharmacological benefits such as anti-inflammatory and anti-oxidant effects. Since these effects may improve sarcopenia and the functions of satellite cells, we examined the effects of coffee on the skeletal muscles in an animal model using aged mice. In vivo, coffee treatment attenuated the decrease in the muscle weight and grip strength, increased the regenerating capacity of injured muscles, and decreased the serum pro-inflammatory mediator levels compared to controls. In vitro, using satellite cells isolated from aged mice, coffee treatment increased the cell proliferation rate, augmented the cell cycle, and increased the activation level of Akt intra-cellular signaling pathway compared to controls. These findings suggest that the coffee treatment had a beneficial effect on age-related sarcopenia.
authors.affiliation: Department of Nutrition and Food Science, School of Public Health, Tianjin Medical University, Tianjin, China; Department of Behavioral Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Yinting
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Nutrition and Food Science, School of Public Health, Tianjin Medical University, Tianjin, China. Electronic address: nkj0809@gmail.com.
authors.forename: Kaijun
authors.initials: K
authors.lastname: Niu
authors.affiliation: Department of Respiratory Medicine, Graduate School of Medicine, Tohoku University, Sendai, Japan.
authors.forename: Tatsuma
authors.initials: T
authors.lastname: Okazaki
authors.affiliation: Department of Nutrition and Food Science, School of Public Health, Tianjin Medical University, Tianjin, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Pharmacology, Graduate School of Medicine, Tohoku University, Sendai, Japan.
authors.forename: Takeo
authors.initials: T
authors.lastname: Yoshikawa
authors.affiliation: Division of Geriatric Pharmacotherapy, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohrui
authors.affiliation: Department of Geriatrics and Gerontology, Division of Brain Sciences, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Furukawa
authors.affiliation: Department of Respiratory Medicine, Graduate School of Medicine, Tohoku University, Sendai, Japan.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Ichinose
authors.affiliation: Department of Pharmacology, Graduate School of Medicine, Tohoku University, Sendai, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Yanai
authors.affiliation: Department of Geriatrics and Gerontology, Division of Brain Sciences, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Arai
authors.affiliation: Department of Nutrition and Food Science, School of Public Health, Tianjin Medical University, Tianjin, China.
authors.forename: Guowei
authors.initials: G
authors.lastname: Huang
authors.affiliation: Division of Biomedical Engineering for Health & Welfare, Tohoku University Graduate School of Biomedical Engineering, Sendai, Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Nagatomi
mesh_terms: D000375:Aging / Q000473:pathology / Q000502:physiology; D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D003069:Coffee; D018450:Disease Progression; D004353:Drug Evaluation, Preclinical / Q000379:methods; D018737:Hand Strength; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000293:injuries / Q000473:pathology / Q000502:physiology; D009929:Organ Size / Q000187:drug effects; D008517:Phytotherapy / Q000379:methods*; D012038:Regeneration / Q000187:drug effects; D055948:Sarcopenia / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D032496:Satellite Cells, Skeletal Muscle / Q000473:pathology / Q000502:physiology
chemical_list: D003069:Coffee; D018836:Inflammation Mediators
keywords: Aged mice; Akt signaling; Coffee; IL-6; Inflammation levels; Sarcopenia; Satellite cells; TA; TNF-α; eMyHC; embryonic myosin heavy chain; interleukin 6; tibialis anterior; tumor necrosis factor-alpha