title: [Association between diarrhea-predominant irritable bowel syndrome and HTR3A, HTR3E gene polymorphism in Yangzhou,Jiangsu province, China].
abstract: OBJECTIVE
  To investigate the relationship between diarrhea-predominant irritable bowel syndrome (D-IBS) and HTR3A, HTR3E gene polymorphism in Yangzhou,Jiangsu province.
  
  
  METHODS
  Polymerase chain reaction (PCR) amplification and restriction fragment length polymorphism (RFLP)technique were used to detect the 5'-UTR variant c.-42C > T of HTR3A and 3'-UTR variant c. ∗ 76G > A of HTR3E on 300 healthy subjects and 450 patients with D-IBS.
  
  
  RESULTS
  There was significant difference noticed between the D-IBS patients and the controls in the genotype of c.-42C > T sites (P<0.05), while the frequency of T allele was significantly higher in both female and male patients than that in the controls (P < 0.05). The frequencies of G/A genotype and A allele of c. ∗ 76G > A sites were significantly higher in the female-patient group than that in the controls (P < 0.05), however, there was no significant difference between those male patients and controls(P > 0.05). When comparing to the CC genotype of rs1062613 site and GG genotype of rs 62625044 site, the TT and GA genotype were closely related to the D-IBS, with the risk coefficients as 0.29 (95% CI:0.14-0.61)and 0.62 (95% CI:0.39-0.99), with statistically significant differences.
  
  
  CONCLUSIONS
  T allele and GA genotype might respectively serve as the predisposing factors of D-IBS and on the female D-IBS patients.
authors.affiliation: Medical College, Yangzhou University, Yangzhou 225009, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yao
authors.initials: Y
authors.lastname: Huang
authors.forename: Ping
authors.initials: P
authors.lastname: Bo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D003967:Diarrhea; D005260:Female; D005838:Genotype; D006801:Humans; D043183:Irritable Bowel Syndrome / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D044406:Receptors, Serotonin, 5-HT3 / Q000235:genetics*
chemical_list: D044406:Receptors, Serotonin, 5-HT3