title: Multiple amino acid-excluded genetic codes for protein engineering using multiple sets of tRNA variants.
abstract: A "simplified genetic code", with only 19 amino acids assigned to the sense codons, was recently developed. In this study, we describe novel simplified codes in which multiple amino acids are simultaneously excluded from the universal code. In the simplest code, tryptophan, cysteine, tyrosine, and asparagine codons are assigned to serine by using four kinds of tRNA (Ser) variants. The results revealed that various sets of amino acids can easily be excluded from the universal code, using our strategy for genetic code simplification. A simplified genetic code is useful as an engineering tool for the improvement of industrial enzymes and pharmaceuticals, and also provides new insights into the assessment of protein evolution. Simplified codes in which multiple amino acids are simultaneously excluded from the code can be more effective tools than codes excluding only one amino acid.
authors.affiliation: Department of Computational Intelligence and Systems Science, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology , Midori-ku, Yokohama-shi, Kanagawa 226-8503, Japan.
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Amikura
authors.forename: Yoko
authors.initials: Y
authors.lastname: Sakai
authors.forename: Shun
authors.initials: S
authors.lastname: Asami
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kiga
mesh_terms: D000596:Amino Acids / Q000235:genetics*; D003062:Codon / Q000235:genetics*; D019143:Evolution, Molecular; D008957:Models, Genetic; D015202:Protein Engineering / Q000379:methods*; D012343:RNA, Transfer / Q000235:genetics*; D058615:Synthetic Biology
chemical_list: D000596:Amino Acids; D003062:Codon; D012343:RNA, Transfer