title: The introduction of CMS mitochondria to triazine tolerant Brassica napus L., var. "Regent", by micromanipulation of individual heterokaryons.
abstract: Cytoplasmic triazine tolerance and cytoplasmic male sterility traits were combined in the nuclear genomic background of the Brassica napus variety 'Regent', following protoplast fusion, selection of fusion products by manual micro-manipulation, and culture in a Nicotiana tabacum nurse system. Whole plant cybrid regenerants were morphologically normal and produced seed on pollination, demonstrating their potential for incorporation into a breeding program.
authors.affiliation: Division of Plant Biology, Allelix Inc., 6850 Goreway Drive, L4V 1P1, Mississauga, Ontario, Canada.
authors.forename: S A
authors.initials: SA
authors.lastname: Yarrow
authors.forename: S C
authors.initials: SC
authors.lastname: Wu
authors.forename: T L
authors.initials: TL
authors.lastname: Barsby
authors.forename: R J
authors.initials: RJ
authors.lastname: Kemble
authors.forename: J F
authors.initials: JF
authors.lastname: Shepard