title: Modulation of melanin synthesis by rengyolone isolated from the root of Eurya emarginata in melan-a cells.
abstract: In the course of screening for the melanogenesis inhibitors, rengyolone was isolated from Eurya emarginata (Thumb) Makino. Its chemical structure was determined on the basis of spectroscopic analysis including mass spectroscopy and nuclear magnetic resonance analysis. Rengyolone inhibited potent melanogenesis in melan-a cells with an IC50 value of 65 μM without cytotoxicity. Also, rengyolone showed a melanin biosynthesis inhibition zone in a culture plate of Streptomyces bikiniensis, which is commonly used as an indicator organism. Moreover, rengyolone dramatically reduced protein expression of melanogenic enzyme, tyrosinase. Furthermore, rengyolone presented inhibition on the body pigmentation in zebrafish model system and decreased melanin contents and tyrosinase activity. These results suggest that rengyolone isolated from E. emarginata may be an effective skin-whitening agent that regulates the expression of melanogenic enzymes.
authors.affiliation: Department of Herbal Skin Care, Daegu Haany University, Gyeongsan, Gyeongbuk, 712-715, Korea.
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Sang Chul
authors.initials: SC
authors.lastname: Jeong
authors.forename: Jae Sung
authors.initials: JS
authors.lastname: Hwang
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Lee
authors.forename: Sang Myung
authors.initials: SM
authors.lastname: Lee
mesh_terms: D000818:Animals; D002460:Cell Line; D005663:Furans / Q000494:pharmacology*; D006574:Heterocyclic Compounds, 2-Ring / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D019684:Magnoliopsida / Q000737:chemistry*; D008543:Melanins / Q000096:biosynthesis*; D008544:Melanocytes / Q000187:drug effects* / Q000201:enzymology; D008810:Mice, Inbred C57BL; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors; D010858:Pigmentation / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D013302:Streptomyces / Q000187:drug effects; D015027:Zebrafish
chemical_list: D005663:Furans; D006574:Heterocyclic Compounds, 2-Ring; D008543:Melanins; D010936:Plant Extracts; C509673:Rengyolone; D014442:Monophenol Monooxygenase
keywords: Eurya emarginata; melanogenesis; rengyolone; tyrosinase