title: Cancer-suppressive potential of extracts of endemic plant Helichrysum zivojinii: effects on cell migration, invasion and angiogenesis.
abstract: Helichrysum zivojinii Cernjavski & Soska is an endemic plant species that grows in the National Park Galicica in Macedonia. Five extracts were isolated as fractions from the aerial parts of the plant: a n-hexane extract (1), a dichloromethane extract (2), an ethyl-acetate extract (3), a n-butanol extract (4) and a methanol extract (5). A dose-dependent cytotoxic activity of the extracts on MDA-MB-231 and EA.hy926 cells was observed. Extracts exhibited more pronounced cytotoxic actions on MDA-MB-231 cells than on EA.hy926 cells. The n-hexane extract (1), at a non-toxic concentration, exhibited an inhibitory effect on the migration as well the invasiveness of MDA-MB-231 cells. The dichloromethane extract (2), at a non-toxic concentration, demonstrated inhibition of MDA-MB-231 cells invasion. Each of the five extracts applied at non-toxic concentrations inhibited migration of EA.hy926 cells. The prominent inhibitory effect of the n-hexane extract on EA.hy926 cells migration was associated with a notable anti-angiogenic action of this extract. The other four tested extracts demonstrated mild anti-angiogenic activity. Our data highlight the prominent anticancer potential of n-hexane (1) and dichloromethane (2) extracts, which could be attributed to their very pronounced and selective cytotoxic activities as well as their anti-invasive and anti-angiogenic properties.
authors.affiliation: Institute of Oncology and Radiology of Serbia, Pasterova 14, 11000 Belgrade, Serbia.
authors.forename: Ivana Z
authors.initials: IZ
authors.lastname: Matić
authors.forename: Ivana
authors.initials: I
authors.lastname: Aljancić
authors.forename: Vlatka
authors.initials: V
authors.lastname: Vajs
authors.forename: Milka
authors.initials: M
authors.lastname: Jadranin
authors.forename: Nevenka
authors.initials: N
authors.lastname: Gligorijević
authors.forename: Slobodan
authors.initials: S
authors.lastname: Milosavljević
authors.forename: Zorica D
authors.initials: ZD
authors.lastname: Juranić
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D036562:Helichrysum / Q000737:chemistry*; D006801:Humans; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic; D010936:Plant Extracts / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts