title: Stimulation of airway neutrophils following dexamethasone administration and equid herpesvirus-2 challenge in horses.
abstract: The aim of this study was to investigate neutrophil stimulation following experimentally-induced airway inflammation in healthy horses. Six horses received dexamethasone and four were then inoculated with equid herpesvirus-2 (EHV-2). Significant neutrophilia was detected in tracheal wash and bronchoalveolar lavage fluid for up to 6 days. Concentrations of neutrophil elastase (NE) and myeloperoxidase (MPO) were significantly increased compared to baseline for up to 14 days in tracheal washes and both markers were significantly correlated with neutrophil counts. Serum levels of surfactant protein D were not significantly modified throughout the study. These results suggest that dexamethasone administration with or without EHV-2 inoculation is associated with a sustainable activation and degranulation of neutrophils in the trachea along with moderate modifications detectable in the lower airways.
authors.affiliation: Normandie Université, UNICAEN, SF 4206 ICORE, Frank Duncombe Laboratory, 1 Route de Rosel, 14053 Caen cedex 4, France. Electronic address: eric.richard@calvados.fr.
authors.forename: Eric A
authors.initials: EA
authors.lastname: Richard
authors.affiliation: Normandie Université, UNICAEN, SF 4206 ICORE, Frank Duncombe Laboratory, 1 Route de Rosel, 14053 Caen cedex 4, France.
authors.forename: Pierre-Hugues
authors.initials: PH
authors.lastname: Pitel
authors.affiliation: Merial S.A.S., 29 Avenue Tony Garnier, 69007 Lyon, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Lemaitre
authors.affiliation: Merial S.A.S., 29 Avenue Tony Garnier, 69007 Lyon, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Jas
authors.affiliation: Department of Physiology, Faculty of Veterinary Medicine, University of Liège, 20 Boulevard de Colonster, Bat. B42, 4000 Liège, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Lekeux
authors.affiliation: Normandie Université, UNICAEN, SF 4206 ICORE, Frank Duncombe Laboratory, 1 Route de Rosel, 14053 Caen cedex 4, France; Normandie Université, UNICAEN, EA 4655 - U2RM, 14032 Caen, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Pronost
authors.affiliation: Normandie Université, UNICAEN, SF 4206 ICORE, Frank Duncombe Laboratory, 1 Route de Rosel, 14053 Caen cedex 4, France; Normandie Université, UNICAEN, EA 4655 - U2RM, 14032 Caen, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Fortier
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D003907:Dexamethasone / Q000633:toxicity*; D005260:Female; D005938:Glucocorticoids / Q000633:toxicity; D006566:Herpesviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D006734:Horse Diseases; D006736:Horses; D007249:Inflammation / Q000662:veterinary; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D012137:Respiratory System / Q000166:cytology*; D012141:Respiratory Tract Infections / Q000473:pathology / Q000662:veterinary / Q000821:virology; D018144:Rhadinovirus; D014412:Tumor Virus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology
chemical_list: D005938:Glucocorticoids; D003907:Dexamethasone
keywords: Airway inflammation; Corticosteroids; Horse; Myeloperoxidase; Neutrophil elastase