title: KIFC3 promotes mitotic progression and integrity of the central spindle in cytokinesis.
abstract: Kinesin-14 motor proteins play a variety of roles during metaphase and anaphase. However, it is not known whether members of this family of motors also participate in the dramatic changes in mitotic spindle organization during the transition from telophase to cytokinesis. We have identified the minus-end-directed motor, KIFC3, as an important contributor to central bridge morphology at this stage. KIFC3's unique motor-dependent localization at the central bridge allows it to congress microtubules, promoting efficient progress through cytokinesis. Conversely, when KIFC3 function is perturbed, abscission is delayed, and the central bridge is both widened and extended. Examination of KIFC3 on growing microtubules in interphase indicates that it caps microtubules released from the centrosome, both in the region of the centrosome and in the cell periphery. In line with other kinesin-14 family members, KIFC3 may guide free microtubules to their destination at the bridge and/or may slide and crosslink central bridge microtubules in order to stage the cells for abscission.
authors.affiliation: Department of Developmental and Molecular Biology; Albert Einstein College of Medicine; New York, NY USA.
authors.forename: Jeannette
authors.initials: J
authors.lastname: Nachbar
authors.affiliation: Department of Developmental and Molecular Biology; Albert Einstein College of Medicine; New York, NY USA.
authors.forename: Francisco
authors.initials: F
authors.lastname: Lázaro-Diéguez
authors.affiliation: University of Colorado Medical School, Aurora, CO, USA.
authors.forename: Rytis
authors.initials: R
authors.lastname: Prekeris
authors.affiliation: Department of Developmental and Molecular Biology; Albert Einstein College of Medicine; New York, NY USA.
authors.forename: David
authors.initials: D
authors.lastname: Cohen
authors.affiliation: Department of Developmental and Molecular Biology; Albert Einstein College of Medicine; New York, NY USA.
authors.forename: Anne
authors.initials: A
authors.lastname: Müsch
mesh_terms: D000705:Anaphase / Q000502:physiology*; D000818:Animals; D018385:Centrosome / Q000378:metabolism; D048749:Cytokinesis / Q000502:physiology*; D004285:Dogs; D006367:HeLa Cells; D006801:Humans; D016547:Kinesins / Q000378:metabolism*; D061985:Madin Darby Canine Kidney Cells; D008677:Metaphase / Q000502:physiology*; D008870:Microtubules / Q000378:metabolism; D008941:Spindle Apparatus / Q000502:physiology*
chemical_list: C586505:Kifc3 protein, human; D016547:Kinesins
keywords: KIFC3; abscission; cytokinesis; kinesin-14; mitotic kinesin