title: Danggui-Jakyak-San ameliorates memory impairment and increase neurogenesis induced by transient forebrain ischemia in mice.
abstract: BACKGROUND
  Danggui-Jakyak-San (DJS), a traditional herbal prescription, has been used to treat insufficient blood supplies. Recently, regenerative medication for the treatment of cerebral ischemia has drawn the attention of many researchers.
  
  
  METHODS
  In this study, we examined whether DJS exerts a neuronal regenerative effect in the hippocampus of a transient forebrain ischemia mice model. Transient forebrain ischemia was induced by bilateral common carotid artery occlusion (BCCAO). Animals were divided into three groups (sham, BCCAO + vehicle, and BCCAO + DJS). To test the effect of DJS on learning and memory, Morris water maze or passive avoidance test was conducted. To test neuroprotective and neurogenic effect, immunohistochemistry and Western blot analysis were used. Statistical significance was analyzed with Student t-test, one-way or two-way analysis of variance.
  
  
  RESULTS
  We found that the administration of DJS ameliorated ischemia-induced spatial memory impairment in the Morris water maze task. Moreover, Akt/glycogen synthase kinase-3β (GSK3β)/β-catenin signaling was increased by DJS, which would be one possible mechanism of DJS for neurogenesis in the hippocampal dentate gyrus region.
  
  
  CONCLUSIONS
  These results suggest that DJS is a possible candidate for the treatment of ischemia-induced neuronal degeneration.
authors.forename: Mi Deok
authors.initials: MD
authors.lastname: Song
authors.forename: Dong Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Jong Min
authors.initials: JM
authors.lastname: Kim
authors.forename: Hyung Eun
authors.initials: HE
authors.lastname: Lee
authors.forename: Se Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Graduate School of East-west Medical Science, Kyung Hee University, Yongin 446-701, Republic of Korea. jhryu63@khu.ac.kr.
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Ryu
authors.forename: Jae Hwan
authors.initials: JH
authors.lastname: Lew
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000150:complications*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects; D006801:Humans; D002546:Ischemic Attack, Transient / Q000150:complications*; D008297:Male; D008569:Memory Disorders / Q000188:drug therapy* / Q000209:etiology / Q000503:physiopathology; D051379:Mice; D008810:Mice, Inbred C57BL; D055495:Neurogenesis / Q000187:drug effects*; D010936:Plant Extracts / Q000008:administration & dosage*
chemical_list: D010936:Plant Extracts