title: Preparation of polysaccharides from cyanobacteria Nostoc commune and their antioxidant activities.
abstract: In this study, water soluble polysaccharides were prepared from cyanobacteria Nostoc commune by water extraction. Factors affecting the polysaccharide yields were investigated, and the optimum extraction conditions were determined as follows: time, 4h; temperature, 90 °C; the ratio of liquid to solid, 60:1 (v/w); and extraction times, 4. The extract was filtered, concentrated to ∼10% (w/v), precipitated with 3 volumes of ethanol, freeze-dried, and ground to yield a water soluble power. The polysaccharide content of the product was 96.7%, and the yield was 9.18% (w/w). Fourier transform infrared spectra demonstrated that the product samples were mainly composed of polysaccharides. The polysaccharides showed high hydroxyl radical scavenging activity (92.71%) and reducing capacity (0.445) at the concentration of 10 mg/mL.
authors.affiliation: School of Food Engineering, Huaihai Institute of Technology, 59 Cangwu Road, Xinpu 222005, China.
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Wang
authors.forename: Sheng-Jun
authors.initials: SJ
authors.lastname: Wu
authors.forename: Dou
authors.initials: D
authors.lastname: Liu
mesh_terms: D000431:Ethanol / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification*; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors; D050501:Nostoc commune / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000302:isolation & purification*; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D016166:Free Radical Scavengers; D011135:Polysaccharides, Bacterial; D014867:Water; D017665:Hydroxyl Radical; D000431:Ethanol
keywords: Antioxidant activities; Extraction; Polysaccharides