title: Role of vitamin D on the expression of glucose transporters in L6 myotubes.
abstract: Altered expression of glucose transporters is a major characteristic of diabetes. Vitamin D has evolved widespread interest in the pathogenesis and prevention of diabetes. The present study was designed to investigate the effect of vitamin D in the overall regulation of muscle cell glucose transporter expression. L6 cells were exposed to type 1 and type 2 diabetic conditions and the effect of calcitriol (1,25, dihydroxy cholicalciferol) on the expression of glucose transporters was studied by real time polymerase chain reaction (RT-PCR). There was a significant decrease in glucose transporter type 1 (GLUT1), GLUT4, vitamin D receptor (VDR), and IR expression in type 1 and 2 diabetic model compared to control group. Treatment of myoblasts with 10-7 M calcitriol for 24 h showed a significant increase in GLUT1, GLUT4, VDR, and insulin receptor (IR) expression. The results indicate a potential antidiabetic function of vitamin D on GLUT1, GLUT4, VDR, and IR by improving receptor gene expression suggesting a role for vitamin D in regulation of expression of the glucose transporters in muscle cells.
authors.affiliation: Department of Endocrinology, Diabetes and Metabolism, Sri Ramachandra University, Chennai, Tamil Nadu, India.
authors.forename: Bubblu
authors.initials: B
authors.lastname: Tamilselvan
authors.forename: Krishna G
authors.initials: KG
authors.lastname: Seshadri
authors.forename: Ganesh
authors.initials: G
authors.lastname: Venkatraman
keywords: Diabetes; glucose transporters; vitamin D