title: Adenovirus type 3 induces platelet activation in vitro.
abstract: In the present study, we aimed to investigate platelet activation induced by adenovirus type 3 (HAdV3) in vitro. Platelet-rich plasma (PRP) or whole blood was incubated with or without HAdV at various concentrations. Platelet aggregation, platelet counting, fibrinogen and expression of platelet membrane antigens (CD41a and CD62P) were determined following incubation with HAdV for different periods of time. The results demonstrated that HAdV at the concentrations of 109-1011 vp/ml enhanced adenosine diphosphate (ADP) or ristocetin-induced platelet aggregation, however did not alter the platelet count. Infection with HAdVs also reduced fibrinogen level. P-selectin and CD41a appeared rapidly on the surface after platelets were incubated with HAdVs in vitro for 30 min. In conclusion, HAdVs may induce activation of platelets and lead to a pre-thrombotic state of peripheral blood. This finding may aid in the development of measures to prevent severe HAdV infection.
authors.affiliation: Department of Hygienic Microbiology, Public Health College, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Ying-Yu
authors.initials: YY
authors.lastname: Jin
authors.forename: Xiu-Nan
authors.initials: XN
authors.lastname: Yu
authors.forename: Zhang-Yi
authors.initials: ZY
authors.lastname: Qu
authors.forename: Ai-Ai
authors.initials: AA
authors.lastname: Zhang
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Xing
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Jiang
authors.forename: Lei
authors.initials: L
authors.lastname: Shang
authors.forename: Ying-Chen
authors.initials: YC
authors.lastname: Wang
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000256:Adenoviridae / Q000502:physiology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D019007:P-Selectin / Q000378:metabolism; D015539:Platelet Activation / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D010976:Platelet Count; D040201:Platelet Membrane Glycoprotein IIb / Q000378:metabolism; D012310:Ristocetin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D019007:P-Selectin; D040201:Platelet Membrane Glycoprotein IIb; D012310:Ristocetin; D000244:Adenosine Diphosphate; D005340:Fibrinogen