title: Antioxidant activity and protective effects of Trapa japonica pericarp extracts against tert-butylhydroperoxide-induced oxidative damage in Chang cells.
abstract: In this study, the antioxidant properties of Trapa japonica pericarp extracts were evaluated through several biochemical assays: 2,2-diphenyl-1-picrylhydrazyl (DPPH), alkyl radical scavenging activity, hydroxyl radical scavenging, ferric reducing antioxidant power (FRAP) assay, ABTS radical scavenging activity and oxygen radical absorbance capacity (ORAC). The antioxidant activities were compared with other natural and synthetic antioxidants. The results showed that higher radical scavenging activity and antioxidant capacity in FRAP than those of vitamin C as a positive control. T. japonica pericarp extracts have antioxidant properties through its ability to prevent tert-butylhydroperoxide (t-BHP)-induced toxicity which enhance the cell viability, reduce reactive oxygen species (ROS) production, inhibits of oxidative damage and mitochondria dysfunction in Chang liver cells. Therefore, based on these finding, it seems reasonable to suggest that T. japonica pericarp extracts has the potential to protect liver against t-BHP-induced cell damage and should be considered as a potential functional food.
authors.affiliation: Department of Biotechnology, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea.
authors.forename: Yon-Suk
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea.
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Hwang
authors.affiliation: Department of Biotechnology, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea.
authors.forename: Young-Ki
authors.initials: YK
authors.lastname: Han
authors.affiliation: Korea Food Research Institute, Seong-Nam, Gyeonggi 463-746, Republic of Korea.
authors.forename: Hyuck-Ju
authors.initials: HJ
authors.lastname: Kwon
authors.affiliation: Department of Medical Science, School of Medicine Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Heeok
authors.initials: H
authors.lastname: Hong
authors.affiliation: Department of Acupoint and Meridian, College of Oriental Medicine, Semyung University, Jecheon, Chungbuk 390-711, Republic of Korea.
authors.forename: Ee-Hwa
authors.initials: EH
authors.lastname: Kim
authors.affiliation: Nokyong Research Center, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea.
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Moon
authors.affiliation: Nokyong Research Center, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea.
authors.forename: Byong-Tae
authors.initials: BT
authors.lastname: Jeon
authors.affiliation: Department of Biotechnology, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea; Nokyong Research Center, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea. Electronic address: parkpj@kku.ac.kr.
authors.forename: Pyo-Jam
authors.initials: PJ
authors.lastname: Park
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001562:Benzimidazoles / Q000737:chemistry; D052160:Benzothiazoles / Q000737:chemistry; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D029561:Lythraceae / Q000737:chemistry*; D053078:Membrane Potential, Mitochondrial; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013451:Sulfonic Acids / Q000737:chemistry; D020122:tert-Butylhydroperoxide / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D001562:Benzimidazoles; D052160:Benzothiazoles; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D020122:tert-Butylhydroperoxide; C017807:bisbenzimide ethoxide trihydrochloride
keywords: Antioxidant activity; Chang cells; Free radical; Oxidative stress; Trapa japonica pericarp; t-BHP