title: Antimicrobial susceptibilities of Mycoplasma isolated from bovine mastitis in Japan.
abstract: Mycoplasma spp. are highly contagious pathogens and intramammary Mycoplasma infection is a serious issue for the dairy industry. As there is no effective vaccine for Mycoplasma infection, control depends on good husbandry and chemo-antibiotic therapy. In this study, antimicrobial susceptibility of Mycoplasma strains recently isolated from cases of bovine mastitis in Japan was evaluated by minimum inhibitory concentration (MIC). All Mycoplasma bovis strains were sensitive to pirlimycin, danofloxacin and enrofloxacin, but not kanamycin, oxytetracycline, tilmicosin or tylosin. M. californicum and M. bovigenitalium strains were sensitive to pirlimycin, danofloxacin, enrofloxacin, oxytetracycline, tilmicosin and tylosin, but not to kanamycin. This is the first report to describe the MIC of major antimicrobial agents for Mycoplasma species isolated from bovine mastitis in Japan.
authors.affiliation: Department of Animal Health 1, School of Veterinary Medicine, Azabu University, Sagamihara, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kawai
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Higuchi
authors.forename: Hidetomo
authors.initials: H
authors.lastname: Iwano
authors.forename: Akihiro
authors.initials: A
authors.lastname: Iwakuma
authors.forename: Ken
authors.initials: K
authors.lastname: Onda
authors.forename: Reiichiro
authors.initials: R
authors.lastname: Sato
authors.forename: Tomohito
authors.initials: T
authors.lastname: Hayashi
authors.forename: Hajime
authors.initials: H
authors.lastname: Nagahata
authors.forename: Toshio
authors.initials: T
authors.lastname: Oshida
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002417:Cattle; D002981:Clindamycin / Q000031:analogs & derivatives* / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D000077422:Enrofloxacin; D005260:Female; D024841:Fluoroquinolones / Q000494:pharmacology*; D007564:Japan; D008414:Mastitis, Bovine / Q000382:microbiology*; D009174:Mycoplasma / Q000187:drug effects* / Q000302:isolation & purification*; D009175:Mycoplasma Infections; D010118:Oxytetracycline / Q000494:pharmacology*; D015645:Tylosin / Q000031:analogs & derivatives* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; C068581:danofloxacin; D000077422:Enrofloxacin; D002981:Clindamycin; C034093:pirlimycin; D010118:Oxytetracycline; C052319:tilmicosin; D015645:Tylosin
keywords: Mycoplasma; antimicrobial agent; mastitis; minimum inhibitory concentration