title: Survey of extra-intestinal immune responses in asymptomatic long-term Campylobacter jejuni-infected mice.
abstract: Campylobacter jejuni is among the most frequently reported bacterial pathogens causing diarrhea in humans worldwide. We recently reported a murine infection model mimicking key features of human campylobacteriosis. Six days following oral C. jejuni infection immediately after weaning, infant mice developed acute enterocolitis resolving within 2 weeks. Thereafter, C. jejuni could still be isolated from the intestines of asymptomatic mice at low levels accompanied by distinct immune responses, both at intestinal and extra-intestinal locations. We here show that, at day 103 post infection (p.i.), long-term C. jejuni-infected mice exhibited higher numbers of T lymphocytes in liver, lung, kindneys, and cardiac muscle as compared to uninfected controls. In addition, B lymphocytes were slightly higher, but macrophage numbers were significantly lower in liver and lung of C. jejuni-infected versus naive mice. As compared to uninfected control animals, proliferating cells were significantly lower in liver, lung, kidneys, cardiac muscle, and spleen at day 103 p.i., whereas more apoptotic cells were abundant in the spleen with predominance in the red pulp. This study underlines that post-infectious, immunological sequelae at extra-intestinal locations are of importance even in asymptomatic long-term C. jejuni carriers and need to be further studied in order to unravel the underlying molecular mechanisms.
authors.forename: Markus M
authors.initials: MM
authors.lastname: Heimesaat
authors.forename: Lea-Maxie
authors.initials: LM
authors.lastname: Haag
authors.forename: André
authors.initials: A
authors.lastname: Fischer
authors.forename: Bettina
authors.initials: B
authors.lastname: Otto
authors.forename: Anja A
authors.initials: AA
authors.lastname: Kühl
authors.forename: Ulf B
authors.initials: UB
authors.lastname: Göbel
authors.forename: Stefan
authors.initials: S
authors.lastname: Bereswill
keywords: B lymphocytes; Campylobacter jejuni; T lymphocytes; apoptotic cells; colonization resistance; extra-intestinal immune cell influx; host–pathogen interaction; infant mice; innate and adaptive immunity; intestinal microbiota; long-term C. jejuni infection; macrophages; proliferative cells