title: The dynamics of soluble Fas/APO 1 apoptotic biochemical marker in acute ischemic stroke patients.
abstract: OBJECTIVE
  Until recently, neuronal death in ischemic stroke infarction was ascribed exclusively to necrotic process. However, experimental animal models of cerebral ischemia suggest apoptosis to play a role in the pathogenesis of cerebral infarction. The aim of this study was to determine the level and monitor the dynamics of soluble Fas/APO 1 (sFas/APO 1) in serum and cerebrospinal fluid of acute ischemic stroke patients.
  
  
  METHODS
  This prospective study included 23 patients with first ever, computed tomography verified acute ischemic stroke and 20 control subjects with other functional neurologic disorders. Serum and cerebrospinal fluid sFas/APO 1 levels were determined on several occasions. Blood samples were obtained on day 1, 3 and 12, and lumbar puncture on day 3 and 12 of disease onset. Quantitative sandwich ELISA method was used on sFas/APO 1 determination.
  
  
  RESULTS
  On day 1 of disease onset, serum and cerebrospinal fluid sFas/APO 1 levels were significantly higher in stroke patients as compared to control subjects, and then gradually declined during the period of monitoring.
  
  
  CONCLUSIONS
  Study results confirmed the dynamic pattern of sFas/APO 1 in serum and cerebrospinal fluid of patients with acute ischemic stroke, suggesting the possible role of apoptosis in the pathogenesis of cerebral infarction.
authors.forename: D
authors.initials: D
authors.lastname: Mahovic
authors.forename: N
authors.initials: N
authors.lastname: Zurak
authors.forename: N
authors.initials: N
authors.lastname: Lakusic
authors.forename: D
authors.initials: D
authors.lastname: Sporis
authors.forename: N
authors.initials: N
authors.lastname: Zarkovic
authors.forename: N
authors.initials: N
authors.lastname: Stancin
authors.forename: M
authors.initials: M
authors.lastname: Bosnar-Puretic
mesh_terms: D000368:Aged; D017209:Apoptosis / Q000502:physiology; D015415:Biomarkers / Q000097:blood / Q000134:cerebrospinal fluid; D002545:Brain Ischemia / Q000000981:diagnostic imaging / Q000209:etiology / Q000378:metabolism*; D002544:Cerebral Infarction / Q000000981:diagnostic imaging / Q000209:etiology / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D020521:Stroke / Q000000981:diagnostic imaging / Q000209:etiology / Q000378:metabolism*; D014057:Tomography, X-Ray Computed; D019014:fas Receptor / Q000097:blood* / Q000134:cerebrospinal fluid*
chemical_list: D015415:Biomarkers; C491901:FAS protein, human; D019014:fas Receptor