title: Analysis of microRNA expression profiles in human hepatitis B virus-related hepatocellular carcinoma.
abstract: BACKGROUND
  Increasing evidence has shown that the deregulation of microRNAs (miRNAs) is closely related to the development and progression of hepatocellular carcinoma (HCC). To screen for HCC-specific miRNAs, this study investigated the differentially expressed miRNAs between HCC and matched non-tumorous tissue (NT).
  
  
  METHODS
  This study analyzed the differential expression profiles of miRNAs in 11 pairs of HCC and matched NT from 11 hepatitis B virus (HBV) infection patients with the RT2 miRNA PCR array containing 88 human cancer-related miRNAs. The fold change value was more than two between the HCC and the matched NT, which indicated that there was deregulation of miRNAs. The down-regulated let-7a was validated in another sample set of 34 tissues with the TaqMan RT-qPCR method.
  
  
  RESULTS
  Compared with the matched NT tissues, 9 miRNAs were up-regulated in the HCC tissues, and three were considered statistically significant (p < 0.05): miR-96, miR-183, and miR-196a, which were up-regulated 4.746-, 7.127-, and 3.498-fold, respectively. Simultaneously, 9 miRNAs were down-regulated in the HCC tissues, and two were considered statistically significant: let-7c and miR-138, which were down-regulated 3.945- and 4.790-fold, respectively. The expression levels of let-7a were 1.071 +/- 0.401, 0.926 +/- 0.477, 0.881 +/- 1.214, and 0.535 +/- 0.719 in the healthy group, chronic hepatitis B(CHB) group, NT group, and HCC group, respectively (p > 0.05).
  
  
  CONCLUSIONS
  This study demonstrates that 18 miRNAs were deregulated in the HCC and matched NT tissues. The deregulated miRNAs suggest that further analyses with larger miRNA samples as a diagnostic marker are warranted.
authors.affiliation: Third Central Clinical College of Tianjin Medical University, Tianjin 300170, China.
authors.forename: Jianbiao
authors.initials: J
authors.lastname: Li
authors.forename: Wenxia
authors.initials: W
authors.lastname: Shi
authors.forename: Yingtang
authors.initials: Y
authors.lastname: Gao
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.forename: Xiang
authors.initials: X
authors.lastname: Jing
authors.forename: Shigang
authors.initials: S
authors.lastname: Shan
authors.forename: Yijun
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Du
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000821:virology; D015536:Down-Regulation; D020869:Gene Expression Profiling; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000821:virology; D035683:MicroRNAs / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs