title: Geometric control of vimentin intermediate filaments.
abstract: Significant efforts have addressed the role of vimentin intermediate filaments (VIF) in cell motility, shape, adhesion and their connections to microfilaments (MF) and microtubules (MT). The present work uses micropatterned substrates to control the shapes of mouse fibroblasts and demonstrates that the cytoskeletal elements are dependent on each other and that unlike MF, VIF are globally controlled. For example, both square and circle shaped cells have a similar VIF distribution while MF distributions in these two shapes are quite different and depend on the curvature of the shape. Furthermore, in asymmetric and polarized shaped cells VIF avoid the sharp edges where MF are highly localized. Experiments with vimentin null mouse embryonic fibroblasts (MEFs) adherent to polarized (teardrop) and un-polarized (dumbbell) patterns show that the absence of VIF alters microtubule organization and perturbs cell polarity. The results of this study also demonstrate the utility of patterned substrates for quantitative studies of cytoskeleton organization in adherent cells.
authors.affiliation: Departments of Biomedical Engineering and Chemistry, Northwestern University, IL, United States.
authors.forename: Shagufta H
authors.initials: SH
authors.lastname: Shabbir
authors.affiliation: Department of Cell and Molecular Biology, Northwestern University, IL, United States.
authors.forename: Megan M
authors.initials: MM
authors.lastname: Cleland
authors.affiliation: Department of Cell and Molecular Biology, Northwestern University, IL, United States.
authors.forename: Robert D
authors.initials: RD
authors.lastname: Goldman
authors.affiliation: Departments of Biomedical Engineering and Chemistry, Northwestern University, IL, United States.|Department of Cell and Molecular Biology, Northwestern University, IL, United States.
authors.forename: Milan
authors.initials: M
authors.lastname: Mrksich
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007382:Intermediate Filaments / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D014746:Vimentin / Q000235:genetics / Q000378:metabolism*
chemical_list: D014746:Vimentin
keywords: Cell adhesion; Cytoskeleton; Image analysis; Mechanical properties; Micropatterning