title: Location of the replication origin in the 9-kb repeat size class of rDNA in pea (Pisum sativum).
abstract: The replication origin of the 9-kb rDNA repeat size class of pea (Pisum sativum cv. Alaska) was identified by benzoylated naphthoylated DEAE-cellulose column chromatography and Southern blotting procedures. The origin is located at or near a 0.19-kb EcoR I fragment in the non-transcribed spacer region between the 25S and 18S rRNA genes. Identification of the origin was based on three criteria: (i) an enrichment of the 0.19-kb fragment in replicating rDNA from asynchronously dividing root meristematic cells, (ii) the scarcity of the 0.19-kb fragment in rDNA from non-dividing carbohydrate starved cells, and (iii) a 60-min periodic enrichment of the 0.19-kb fragment in replicating rDNA that temporally coincides with the sequential initiation of replication of replicon families in synchronized pea root cells.
authors.affiliation: Biology Department, Brookhaven National Laboratory, 11973, Upton, New York, USA.
authors.forename: J
authors.initials: J
authors.lastname: Van't Hof
authors.forename: P
authors.initials: P
authors.lastname: Hernández
authors.forename: C A
authors.initials: CA
authors.lastname: Bjerknes
authors.forename: S S
authors.initials: SS
authors.lastname: Lamm