title: Totipotency of tomato protoplasts.
abstract: An efficient and reliable protocol for tomato protoplast isolation, culture, and plant regeneration has been developed. Fourteen diverse cultivars were tested. Fertile plants were regenerated from all 14 cultivars without any modification in the protocol. Plating efficiency (percentage of the protoplasts that formed minicalli) of up to 50% was achieved. Those mini-calli rapidly regenerated shoots at high frequencies. Regenerated shoots can be easily rooted on a basal medium with the appropriate auxin, and have been set to soil within two months after the isolation of the protoplasts.
authors.affiliation: Genetics and Tissue Culture Group, ARCO Plant Cell Research Institute, 6560 Trinity Court, 94568, Dublin, CA, USA.
authors.forename: E A
authors.initials: EA
authors.lastname: Shahin