title: Expression profiling of ETO2-regulated miRNAs in erythroid cells: Possible influence on miRNA abundance.
abstract: ETO2 is a component of a protein complex containing master regulators of hematopoiesis, including GATA-1 and SCL/TAL1, and also has RNA binding properties. Although ETO2 has been reported to repress GATA-1 target genes through histone deacetylation of the target gene loci in erythroid cells, little is known about the contribution of ETO2 to microRNA (miRNA) regulation. Here, we conducted miRNA profiling in ETO2-overexpressing and ETO2-silenced K562 cells. The analysis suggests that ETO2 positively regulates the abundance of mature miRNAs, including miR-21, miR-29b and let-7e. Our data suggest a novel mode of ETO2-mediated target gene repression via effects on miRNA expression.
authors.affiliation: Hematology and Rheumatology, Tohoku University Graduate School of Medicine, Sendai, Japan ; Molecular Hematology/Oncology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Fujiwara
authors.forename: Yoko
authors.initials: Y
authors.lastname: Okitsu
authors.forename: Yuna
authors.initials: Y
authors.lastname: Katsuoka
authors.forename: Noriko
authors.initials: N
authors.lastname: Fukuhara
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Onishi
authors.forename: Kenichi
authors.initials: K
authors.lastname: Ishizawa
authors.forename: Hideo
authors.initials: H
authors.lastname: Harigae
keywords: CBF1, core-binding factor 1; ETO2; ETO2 (CBFA2T3), core-binding factor, runt domain, alpha subunit 2, translocated to, 3; Erythropoiesis; IL-3, interleukin 3; IMDM, Iscove’s Modified Dulbecco’s Media; LMO2, LIM domain only 2; RPMI, Roswell Park Memorial Institute; SCF, stem cell factor; cDNA, complementary DNA; miRNA; siRNA, small interfering RNA