title: Regeneration of somatic embryos from protoplasts isolated from an embryogenic suspension culture of white spruce (Picea glauca).
abstract: Regeneration of white spruce (Picea glauca) somatic embryos from protoplasts derived from an embryogenic suspension culture was accomplished using a culture medium containing 2 mgl(-1) 2,4-D and 1 mgl(-1) 6-BAP. Divisions within 2 days led to plating efficiencies in the order of 24% after 9 days. A reduction in the osmoticum, necessary for sustained growth, was carried out gradually over 30 days. Embedding in agarose and culture in 5 cm petri dishes prior to transfer of agarose blocks to a bead type culture, led to the formation of somatic embryos as early as 23 days after isolation and yielded plating efficiencies in the order of 5-10% after 35 days culture.
authors.affiliation: Department of Biology, University of Saskatchewan, S7N 0W0, Saskatoon, Saskatchewan, Canada.
authors.forename: S M
authors.initials: SM
authors.lastname: Attree
authors.forename: F
authors.initials: F
authors.lastname: Bekkaoui
authors.forename: D I
authors.initials: DI
authors.lastname: Dunstan
authors.forename: L C
authors.initials: LC
authors.lastname: Fowke