title: HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES1 is required for circadian periodicity through the promotion of nucleo-cytoplasmic mRNA export in Arabidopsis.
abstract: Cold acclimation has been shown to be attenuated by the degradation of the INDUCER OF CBF EXPRESSION1 protein by the E3 ubiquitin ligase HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES1 (HOS1). However, recent work has suggested that HOS1 may have a wider range of roles in plants than previously appreciated. Here, we show that hos1 mutants are affected in circadian clock function, exhibiting a long-period phenotype in a wide range of temperature and light environments. We demonstrate that hos1 mutants accumulate polyadenylated mRNA in the nucleus and that the circadian defect in hos1 is shared by multiple mutants with aberrant mRNA export, but not in a mutant attenuated in nucleo-cytoplasmic transport of microRNAs. As revealed by RNA sequencing, hos1 exhibits gross changes to the transcriptome with genes in multiple functional categories being affected. In addition, we show that hos1 and other previously described mutants with altered mRNA export affect cold signaling in a similar manner. Our data support a model in which altered mRNA export is important for the manifestation of hos1 circadian clock defects and suggest that HOS1 may indirectly affect cold signaling through disruption of the circadian clock.
authors.affiliation: Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter EX4 4QD, United Kingdom.
authors.forename: Dana R
authors.initials: DR
authors.lastname: MacGregor
authors.forename: Peter
authors.initials: P
authors.lastname: Gould
authors.forename: Julia
authors.initials: J
authors.lastname: Foreman
authors.forename: Jayne
authors.initials: J
authors.lastname: Griffiths
authors.forename: Susannah
authors.initials: S
authors.lastname: Bird
authors.forename: Rhiannon
authors.initials: R
authors.lastname: Page
authors.forename: Kelly
authors.initials: K
authors.lastname: Stewart
authors.forename: Gavin
authors.initials: G
authors.lastname: Steel
authors.forename: Jack
authors.initials: J
authors.lastname: Young
authors.forename: Konrad
authors.initials: K
authors.lastname: Paszkiewicz
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Millar
authors.forename: Karen J
authors.initials: KJ
authors.lastname: Halliday
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Hall
authors.forename: Steven
authors.initials: S
authors.lastname: Penfield
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D057906:Circadian Clocks / Q000502:physiology*; D003080:Cold Temperature; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D026723:Polyadenylation; D011401:Promoter Regions, Genetic; D050683:R-SNARE Proteins / Q000235:genetics / Q000378:metabolism; D034443:RNA Transport; D012333:RNA, Messenger / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; C425768:HOS1 protein, Arabidopsis; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D050683:R-SNARE Proteins; D012333:RNA, Messenger; C099591:SAR1 protein, Arabidopsis; C504788:LOS4 protein, Arabidopsis; D053487:DEAD-box RNA Helicases