title: Molecular cloning and functional characterization of an endo-β-1,3-glucanase from Streptomyces matensis ATCC 23935.
abstract: A gene encoding an endo-β-1,3-glucanase from Streptomyces matensis ATCC 23935 (SmβG) was characterised by cloning and expressing it in Escherichiacoli. The purified enzyme produced β-1,3-glucooligosaccharides, mainly laminaripentaose, from insoluble curdlan. The optimum pH and temperature were 6.0 and 55 °C, respectively. SmβG inhibited the growth of Candida albicans, which indicates that this enzyme could be potentially used as an anti-fungal agent to control invasive Candida infections. The results suggest that SmβG may be a useful bioavailable β-1,3-glucanase.
authors.affiliation: Department of Food and Nutrition, Brain Korea 21 Project, Yonsei University, Seoul 120-749, Republic of Korea.
authors.forename: Jang-Bin
authors.initials: JB
authors.lastname: Woo
authors.forename: Hae-Na
authors.initials: HN
authors.lastname: Kang
authors.forename: Eui-Jeon
authors.initials: EJ
authors.lastname: Woo
authors.forename: Soo-Bok
authors.initials: SB
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development; D003001:Cloning, Molecular; D004795:Enzyme Stability; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D013302:Streptomyces / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D000935:Antifungal Agents; D001426:Bacterial Proteins; D006026:Glycoside Hydrolases
keywords: Anti-fungal; Candida albicans; Curdlan; Endo-β-1,3-glucanase; β-1,3-Glucooligosaccharide