title: Bean lectins IV: genetic variation in the non-denatured structure of lectins from different Phaseolus vulgaris L. cultivars.
abstract: Variation in the native conformation of bean lectins was examined using electrophoresis of non-denatured total protein extracts and purified albumin and globulin lectin. The observed variation was related to the genetic variation reported previously for lectin polypeptide composition as revealed by two-dimensional isoelectricfocusing-sodium dodecyl sulfate polyacrylamide gel electrophoresis (IEF-SDS/PAGE). When eleven cultivars with different IEF-SDS/PAGE lectin polypeptide compositions were compared, eight had unique non-denatured lectin patterns and three had identical patterns. For some cultivars differences in non-denatured lectin patterns were observed between the purified albumin and globulin lectin preparations.
authors.affiliation: Department of Horticulture, University of Wisconsin, 1575 Linden Drive, 53706, Madison, WI, USA.
authors.forename: T C
authors.initials: TC
authors.lastname: Osborn
authors.forename: J F
authors.initials: JF
authors.lastname: Manen
authors.forename: J W
authors.initials: JW
authors.lastname: Brown
authors.forename: F A
authors.initials: FA
authors.lastname: Bliss