title: Analysis of beat fluctuations and oxygen consumption in cardiomyocytes by scanning electrochemical microscopy.
abstract: The contractile behavior of cardiomyocytes can be monitored by measuring their action potentials, and the analysis is essential for screening the safety of potential drugs. However, immobilizing cardiac cells on a specific electrode is considerably complicated. In this study, we demonstrate that scanning electrochemical microscopy (SECM) can be used to analyze rapid topographic changes in beating cardiomyocytes in a standard culture dish. Various cardiomyocyte contraction parameters and oxygen consumption based on cell respiration could be determined from SECM data. We also confirmed that cellular changes induced by adding the cardiotonic agent digoxin were conveniently monitored by this SECM system. These results show that SECM can be a potentially powerful tool for use in drug development for cardiovascular diseases.
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Toyohira, Sapporo 062-8517, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Hirano
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Toyohira, Sapporo 062-8517, Japan.
authors.forename: Mikie
authors.initials: M
authors.lastname: Kodama
authors.affiliation: Cosmo Bio Co., Ltd., Nishi-Ku, Sapporo, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Shibuya
authors.affiliation: Cosmo Bio Co., Ltd., Nishi-Ku, Sapporo, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Maki
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Toyohira, Sapporo 062-8517, Japan. Electronic address: komatsu-yasuo@aist.go.jp.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Komatsu
mesh_terms: D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology; D019069:Cell Respiration / Q000187:drug effects; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D064347:Microscopy, Electrochemical, Scanning / Q000379:methods*; D009119:Muscle Contraction / Q000187:drug effects; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D013696:Temperature
chemical_list: D002316:Cardiotonic Agents
keywords: Cardiomyocytes; Contraction behavior; Oxygen consumption; Scanning electrochemical microscopy