title: Screening of herbal components for attenuating amiodarone-induced hepatotoxicity on gel-entrapped rat hepatocytes.
abstract: Amiodarone (AMD) is a hepatotoxic drug that has been widely used as a class III antiarrhythmic drug. Because, to date, only a few kinds of protectants are able to reduce AMD hepatotoxicity, this article utilized gel-entrapped rat hepatocytes to screen effective protectants from a series of herbal compounds for their effects against AMD-induced toxicity. Herbal compounds, including matrine, silibinin, glycyrrhizic acid, schisandrin B, epigallocatechin gallate and anisodamine, were cotreated with AMD to assess their protective effect, whereas vitamin E, which has been shown to be protective in rats, was selected as a control. It was found that vitamin E, as with its function in rats, provided the best protection in gel-entrapped rat hepatocytes, whereas silibinin, a major component of silymarin, could largely reduce AMD-induced hepatotoxicity, performing a similar function as silymarin in rats. The results illustrated that gel-entrapped hepatocytes may reflect the protective effects of drugs and serve as a reliable model for screening hepatoprotectants. Moreover, matrine, a widely used monomer of the traditional Chinese medicine, Sophora flavescens, for treatment of arrhythmia, was evidenced to show some effective protections against AMD hepatotoxicity. Taken together, gel-entrapped rat hepatocytes may provide a platform for screening effective candidates from the herbal component library.
authors.affiliation: Department of Chemical and Biological Engineering, Zhejiang University , Zhejiang , China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Deng
authors.forename: Chong
authors.initials: C
authors.lastname: Shen
authors.forename: Qin
authors.initials: Q
authors.lastname: Meng
mesh_terms: D000470:Alkaloids; D000638:Amiodarone / Q000633:toxicity*; D000704:Analysis of Variance; D000818:Animals; D034242:Cyclooctanes / Q000378:metabolism; D055808:Drug Discovery / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D019695:Glycyrrhizic Acid / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects*; D017705:Lignans / Q000378:metabolism; D011083:Polycyclic Compounds / Q000378:metabolism; D011807:Quinolizines; D051381:Rats; D000077385:Silybin; D012838:Silymarin / Q000378:metabolism; D012991:Solanaceous Alkaloids / Q000378:metabolism; D000093842:Matrines
chemical_list: D000470:Alkaloids; D034242:Cyclooctanes; D017705:Lignans; D011083:Polycyclic Compounds; D011807:Quinolizines; D012838:Silymarin; D012991:Solanaceous Alkaloids; C003922:anisodamine; C015499:schizandrin B; D000077385:Silybin; D019695:Glycyrrhizic Acid; D000638:Amiodarone; D000093842:Matrines