title: Melatonin reduces bacterial translocation by preventing damage to the intestinal mucosa in an experimental severe acute pancreatitis rat model.
abstract: Recent studies have demonstrated that melatonin significantly decreased all studied acute pancreatitis-associated inflammatory parameters, in addition to reducing apoptosis and necrosis associated with pancreatic injury. However, the effect of melatonin on gut barrier dysfunction and bacterial translocation has not been fully elucidated. This study aimed to investigate the protective effects of melatonin on intestinal integrity in a rat model of severe acute pancreatitis (SAP) to evaluate whether melatonin prevented intestine barrier dysfunction and reduced bacterial translocation. Forty male Sprague Dawley (SD) rats were randomly divided into three groups, with 8 rats in the sham operation (SO) group, 18 rats in the SAP group and 14 SAP rats in the melatonin treatment (MT) group. SAP was induced by retrograde injection of 4% taurocholate into the biliopancreatic duct. Melatonin was administered 30 min prior to taurocholate injection in the melatonin-treated rats. All rats were sacrificed 24 h subsequent to pancreatitis induction. Real-time fluorescence quantitative polymerase chain reaction was used to detect and quantifyEscherichia coli(E. coli) O157 in postcava blood. The microvilli structure was also analyzed with transmission electron microscopy. The level ofE. coliDNA in the MT group was significantly lower than in rats in the SAP group. NoE. coliDNA was detected in the control group. Villus height and crypt depth in the ileum were significantly higher in the MT and control groups compared to the SAP group, and were significantly higher in the MT group than in the SAP group. These results suggested that melatonin prevented gut barrier dysfunction and reduced bacterial translocation, resulting in reduced pancreatic-associated infections and decreased early mortality rates.
authors.affiliation: Department of Gastroenterology and Hepatology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang 325000, P.R. China.
authors.forename: Xuecheng
authors.initials: X
authors.lastname: Sun
authors.forename: Yingying
authors.initials: Y
authors.lastname: Shao
authors.forename: Yin
authors.initials: Y
authors.lastname: Jin
authors.forename: Jiaping
authors.initials: J
authors.lastname: Huai
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhou
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Huang
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Wu
keywords: acute pancreatitis; bacterial translocation; intestine barrier dysfunction; melatonin; microvilli structure