title: Cord blood banking activity in Iran National Cord Blood Bank: a two years experience.
abstract: Today umbilical cord blood (UCB) has known as a commonly used source of hematopoietic stem cells for allogeneic transplantation and many cord blood banks have been established around the world for collection and cryopreservation of cord blood units. Herein, we describe our experience at Iran National Cord Blood Bank (INCBB) during 2 years of activity. From November 2010 to 2012, UCBs were collected from 5 hospitals in Tehran. All the collection, processing, testing, cryopreservation and storage procedures were done according to standard operation procedures. Total nucleated cells (TNC) count, viability test, CD34+ cell count, colony forming unit (CFU) assay, screening tests and HLA typing were done on all banked units. Within 3770 collected units, only 32.9% fulfilled banking criteria. The mean volume of units was 105.2 ml and after volume reduction the mean of TNC, viability, CD34+ cells and CFUs was 10.76×10(8), 95.2%, 2.99×10(6) and 7.1×10(5), respectively. One unit was transplanted at Dec 2012 to a 5-year old patient with five of six HLA compatibilities. In our country banking of UCB is new and high rate of hematopoietic stem cell transplants needs expanding CB banks capacity to find more matching units, optimization of methods and sharing experiences to improve biological characterization of units.
authors.affiliation: Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran; Iran National Cord Blood Bank, Iran Blood Transfusion Organization, Tehran, Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Jamali
authors.affiliation: Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran; Iran National Cord Blood Bank, Iran Blood Transfusion Organization, Tehran, Iran.
authors.forename: Kamran
authors.initials: K
authors.lastname: Atarodi
authors.affiliation: Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran; Iran National Cord Blood Bank, Iran Blood Transfusion Organization, Tehran, Iran.
authors.forename: Mozhdeh
authors.initials: M
authors.lastname: Nakhlestani
authors.affiliation: Pediatric Congenital Hematologic Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Hasan
authors.initials: H
authors.lastname: Abolghasemi
authors.affiliation: Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
authors.forename: Hosein
authors.initials: H
authors.lastname: Sadegh
authors.affiliation: Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Faranoosh
authors.affiliation: Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran; Iran National Cord Blood Bank, Iran Blood Transfusion Organization, Tehran, Iran.
authors.forename: Khadije
authors.initials: K
authors.lastname: Golzade
authors.affiliation: Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
authors.forename: Razieh
authors.initials: R
authors.lastname: Fadai
authors.affiliation: Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran; Iran National Cord Blood Bank, Iran Blood Transfusion Organization, Tehran, Iran.
authors.forename: Fereshte
authors.initials: F
authors.lastname: Niknam
authors.affiliation: Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran; Iran National Cord Blood Bank, Iran Blood Transfusion Organization, Tehran, Iran. Electronic address: nikougoftar@ibto.ir.
authors.forename: Mahin Nikougoftar
authors.initials: MN
authors.lastname: Zarif
mesh_terms: D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism; D000094902:Blood Banking / Q000379:methods; D002467:Cell Nucleus / Q000378:metabolism; D002470:Cell Survival; D036101:Cord Blood Stem Cell Transplantation; D005260:Female; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology; D006680:HLA Antigens / Q000097:blood / Q000276:immunology; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007492:Iran; D008297:Male; D011247:Pregnancy; D013234:Stem Cells
chemical_list: D018952:Antigens, CD34; D006680:HLA Antigens
keywords: Cord blood bank; Cord blood transplantation; Hematopoietic stem cells