title: SSNMR of biosilica-entrapped enzymes permits an easy assessment of preservation of native conformation in atomic detail.
abstract: Bioinspired silica coprecipitates of enzymes find a wide range of applications for analysis and catalysis in industrial and academic research. Here we used SSNMR as a proxy for the 3D structure of enzymes trapped in bioinspired silica. We show that it is easy to assess whether the enzymes retain their native conformation in atomic detail. We thus propose SSNMR as a rapid and reliable tool to analyze this kind of samples at high resolution.
authors.affiliation: CERM and Department of Chemistry "Ugo Schiff", Via L. Sacconi 6, 50019, Sesto Fiorentino (Fi), Italy. claudioluchinat@cerm.unifi.it.
authors.forename: Marco
authors.initials: M
authors.lastname: Fragai
authors.forename: Claudio
authors.initials: C
authors.lastname: Luchinat
authors.forename: Tommaso
authors.initials: T
authors.lastname: Martelli
authors.forename: Enrico
authors.initials: E
authors.lastname: Ravera
authors.forename: Irit
authors.initials: I
authors.lastname: Sagi
authors.forename: Inna
authors.initials: I
authors.lastname: Solomonov
authors.forename: Yael
authors.initials: Y
authors.lastname: Udi
mesh_terms: D055162:Biocatalysis; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D053508:Matrix Metalloproteinase 12 / Q000737:chemistry / Q000378:metabolism; D008968:Molecular Conformation; D012822:Silicon Dioxide / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000737:chemistry / Q000378:metabolism
chemical_list: D004798:Enzymes; D012822:Silicon Dioxide; D013482:Superoxide Dismutase; D053508:Matrix Metalloproteinase 12