title: Purification of glycollate oxidase from greening cucumber cotyledons.
abstract: Glycollate oxidase (glycollate: oxygen oxidoreductase, EC 1.1.3.1) was purified to apparent homogeneity from crude extracts of greening cucumber cotyledons (Cucumis sat vus). Molecular sieving and chromatofocusing resulted in 700-fold purification and specific activity of 1 μkat mg(-1) protein. The enzyme exhibited a Mr of 180,000, or 700,000, respectively, and is a tetramer or 16-mer made of identical subunits of Mr 43,000. Monospecific antibodies were raised against the homogeneous protein.
authors.affiliation: Biochemie, Fachbereich Chemie der Philipps-Universität, Lahnberge, D-3550, Marburg, Federal Republic of Germany.
authors.forename: W
authors.initials: W
authors.lastname: Behrends
authors.forename: U
authors.initials: U
authors.lastname: Rausch
authors.forename: H G
authors.initials: HG
authors.lastname: Löffler
authors.forename: H
authors.initials: H
authors.lastname: Kindl