title: Lichen secondary metabolites as DNA-interacting agents.
abstract: A series of lichen secondary metabolites (parietin, atranorin, usnic and gyrophoric acid) and their interactions with calf thymus DNA were investigated using molecular biophysics and biochemical methods. The binding constants K were estimated to range from 4.3×10(5) to 2.4×10(7)M(-1) and the percentage of hypochromism was found to be 16-34% (from spectral titration). The results of spectral measurement indicate that the compounds act as effective DNA-interacting agents. Electrophoretic separation studies prove that from all the metabolites tested in this study, only gyrophoric acid exhibited an inhibitory effect on Topo I (25μM).
authors.affiliation: Institute of Chemistry, Department of Biochemistry, Pavol Jozef Šafárik University in Košice, Slovakia.
authors.forename: J
authors.initials: J
authors.lastname: Plsíkova
authors.affiliation: Institute of Biophysics, Department of Molecular Biophysics and Pharmacology, Academy of Science of the Czech Republic, Brno, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Stepankova
authors.affiliation: Institute of Biophysics, Department of Molecular Biophysics and Pharmacology, Academy of Science of the Czech Republic, Brno, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Kasparkova
authors.affiliation: Institute of Biophysics, Department of Molecular Biophysics and Pharmacology, Academy of Science of the Czech Republic, Brno, Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Brabec
authors.affiliation: Institute of Biology and Ecology, Department of Botany, Pavol Jozef Šafárik University in Košice, Slovakia.
authors.forename: M
authors.initials: M
authors.lastname: Backor
authors.affiliation: Institute of Chemistry, Department of Biochemistry, Pavol Jozef Šafárik University in Košice, Slovakia. Electronic address: maria.kozurkova@upjs.sk.
authors.forename: M
authors.initials: M
authors.lastname: Kozurkova
mesh_terms: D000818:Animals; D001565:Benzoates / Q000494:pharmacology; D001572:Benzofurans / Q000494:pharmacology; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000187:drug effects* / Q000378:metabolism; D004642:Emodin / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D062385:Hydroxybenzoates / Q000494:pharmacology; D007364:Intercalating Agents / Q000494:pharmacology; D007700:Kinetics; D008011:Lichens / Q000737:chemistry* / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D013056:Spectrophotometry, Ultraviolet; D059004:Topoisomerase I Inhibitors / Q000494:pharmacology; D059005:Topoisomerase II Inhibitors / Q000494:pharmacology
chemical_list: D001565:Benzoates; D001572:Benzofurans; D062385:Hydroxybenzoates; D007364:Intercalating Agents; D059004:Topoisomerase I Inhibitors; D059005:Topoisomerase II Inhibitors; C073339:usnic acid; C026304:atranorin; D004247:DNA; C427203:calf thymus DNA; C120727:gyrophoric acid; C008905:physcione; D004642:Emodin
keywords: DNA-binding; Lichens; Topoisomerase I, II