title: Genes encoding α-amylase inhibitors are located in the short arms of chromosomes 3B, 3D and 6D of wheat (Triticum aestivum L.).
abstract: Three α-amylase inhibitors, designated Inh. I, II and III have been purified from the 70% ethanol extract of hexaploid wheat (Triticum aestivum L.) and characterized by amino acid analysis, N-terminal amino acid sequencing and enzyme inhibition tests. Inhibitors I and III have identical N-terminal sequences and inhibitory properties to those of the previously described 0.19/0.53 group of dimeric inhibitors. Inhibitor II has an N-terminal sequence which is identical to that of the previously described 0.28 monomeric inhibitor, but differs from it in that in addition to being active against α-amylase from Tenebrio molitor, it is also active against mammalian salivary and pancreatic α-amylases. Compensating nulli-tetrasomic and ditelosomic lines of wheat cv. 'Chinese Spring' have been analysed by two-dimensional electrophoresis, under conditions in which there is no overlap of the inhibitors with other proteins, and the chromosomal locations of the genes encoding these inhibitors have been established: genes for Inh. I and Inh. III are in the short arms of chromosomes 3B and 3D, respectively, and that for Inh. II in the short arm of chromosome 6D.
authors.affiliation: Departamento de Bioquímica, E.T.S. Ingenieros Agrónomos, Ciudad Universitaria, E-28040, Madrid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Sanchez-Monge
authors.forename: D
authors.initials: D
authors.lastname: Barber
authors.forename: E
authors.initials: E
authors.lastname: Mendez
authors.forename: F
authors.initials: F
authors.lastname: García-Olmedo
authors.forename: G
authors.initials: G
authors.lastname: Salcedo