title: Extraction, purification and characterization of polysaccharides from Hawk tea.
abstract: In the present study, the extraction, purification and characterization of polysaccharides from Hawk mature leaf tea (HMP) were investigated. The optimal extraction parameters were obtained by using a Box-Behnken design as follows: extraction temperature 88.9 °C, extraction time 128.2 min and ratio of water to solid 11.4 mL/g. The crude HMP was sequentially purified by chromatography of DEAE-52, and two purified fractions, HMP-1 and HMP-2, were obtained. HMP-1 and HMP-2 were mainly composed of arabinose, galactose, glucose and mannose with the molecular weight of 133 and 100 kDa, respectively. For antioxidant activities in vitro, HMP-1 had strong 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging activity and ferric reducing activity power (FRAP). These results provide a scientific basis for the further use of polysaccharides from this traditional herb tea.
authors.affiliation: College of Life and Basic Sciences, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Xuejing
authors.initials: X
authors.lastname: Jia
authors.forename: Chunbang
authors.initials: C
authors.lastname: Ding
authors.forename: Shu
authors.initials: S
authors.lastname: Yuan
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yang'er
authors.initials: Y
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Du
authors.forename: Ming
authors.initials: M
authors.lastname: Yuan
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D001089:Arabinose / Q000737:chemistry; D001628:Beverages; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D002852:Chromatography, Ion Exchange; D005163:Factor Analysis, Statistical; D005290:Ferric Compounds / Q000737:chemistry; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D059625:Liquid-Liquid Extraction / Q000379:methods / Q000706:statistics & numerical data; D032862:Litsea / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry; D010084:Oxidation-Reduction; D010851:Picrates / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005290:Ferric Compounds; D010851:Picrates; D010936:Plant Extracts; D011134:Polysaccharides; D001089:Arabinose; C004931:1,1-diphenyl-2-picrylhydrazyl; D005947:Glucose; D008358:Mannose; D005690:Galactose
keywords: Antioxidant activity; Litsea coreana var. lanuginose; Polysaccharide; Response surface methodology