title: Differential expressions of G0/G1 switch gene 2 and comparative gene identification-58 are associated with fat content in bovine muscle.
abstract: Regulation of lipolysis in muscle is a potential mechanism affecting marbling in beef carcasses and fat accumulation in muscles of humans, which is a known risk factor for type 2 diabetes. Adipose triglyceride lipase-mediated lipolysis is inhibited by G0/G1 switch gene 2 (G0S2) and co-activated by comparative gene identification-58 (CGI-58). In this study, bovine G0S2 and CGI-58 were sequenced, and expressions of these genes were compared among various tissues and in muscles between bulls and steers with different degrees of marbling. The protein coding sequences of bovine G0S2 and CGI-58 revealed breed-specific SNPs, causing two amino acid variations for each protein. Bovine CGI-58 mRNA showed two isoforms from alternative splicing. The G0S2 gene was preferentially expressed in fat and, to a lesser degree, in the liver; whereas, CGI-58 was highly expressed in the muscle and fat (P < 0.05), suggesting their association with lipid metabolism in those tissues. The longissimus dorsi muscle (LM) of steers showed higher FABP4, G0S2 and CGI-58 mRNA expression levels than the LM of bulls, implying the roles of those genes more in marbling of steers than in that of bulls. The G0S2 expression was markedly higher in the intramuscular fat (IMF) (P < 0.001); whereas, the CGI-58 expression was significantly higher in the pure muscle portion of the LM of steers (P < 0.01), suggesting that G0S2 and CGI-58 may regulate IMF and intramyocellular triglycerides, respectively. Taken together, our data suggest that G0S2 and CGI-58 are associated with fat content in bovine species.
authors.affiliation: Department of Animal Sciences, The Ohio State University, Columbus, OH, 43210, USA.
authors.forename: Jinsoo
authors.initials: J
authors.lastname: Ahn
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Young Min
authors.initials: YM
authors.lastname: Choi
authors.forename: Sangsu
authors.initials: S
authors.lastname: Shin
authors.forename: Shin-Ae
authors.initials: SA
authors.lastname: Oh
authors.forename: Yeunsu
authors.initials: Y
authors.lastname: Suh
authors.forename: Trang Hoa
authors.initials: TH
authors.lastname: Nguyen
authors.forename: Myunggi
authors.initials: M
authors.lastname: Baik
authors.forename: Seongsoo
authors.initials: S
authors.lastname: Hwang
authors.forename: Kichoon
authors.initials: K
authors.lastname: Lee
mesh_terms: D051103:1-Acylglycerol-3-Phosphate O-Acyltransferase / Q000235:genetics*; D000273:Adipose Tissue / Q000378:metabolism*; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D018797:Cell Cycle Proteins / Q000235:genetics*; D050556:Fatty Acid-Binding Proteins / Q000235:genetics; D020869:Gene Expression Profiling; D008297:Male; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018797:Cell Cycle Proteins; D050556:Fatty Acid-Binding Proteins; D051103:1-Acylglycerol-3-Phosphate O-Acyltransferase