title: Oleanolic acid has similar effects as retinoic acid in inducing mouse embryonic stem cell 1B10 to differentiate towards germ cells.
abstract: This study investigated the effects of the compound oleanolic acid (OA) in inducing mouse embryonic stem cells (MESC) to differentiate towards germ cells (GC). MESC 1B10 was used as the model cell. 1B10 was cultured, and embryoid bodies (EBs) were produced from 1B10. The EBs were allowed to attach to the bottoms of culturing disks and grow. OA was added into the medium to induce the EBs to differentiate. Retinoic acid (RA) was used as the positive drug. After 72 h, total RNA was extracted, cDNA was synthesized, and real-time fluorescence quantitative PCR was performed to measure the transcriptional expression profiles of 11 reproduction-related genes affected by OA and RA, respectively. When the data were compared, it was found that OA up-regulated the transcriptional levels of Oct-4, GDF-9, Stra8, Mvh, ZP2, ZP3, Itga6, and TP2, and down-regulated transcriptional levels of SCP3, ZP1, and Itgb1; RA up-regulated the transcriptional levels of GDF-9, Stra8, Mvh, ZP2, ZP3, Itga6, Itgb1, and TP2, and down-regulated the transcriptional levels of Oct-4, SCP3, and ZP1. The data showed that OA and RA had similar effects in inducing differentiation of MESC towards GC.
authors.affiliation: The Reproduction Laboratory of Traditional Chinese Medicine and Western Medicine, The Second Affiliated Clinical Medical School, Chengdu University of Traditional Chinese Medicine, Chengdu, 610041, Sichuan, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wan
authors.forename: Hua
authors.initials: H
authors.lastname: Lu
authors.forename: Yanli
authors.initials: Y
authors.lastname: Deng
authors.forename: Junbei
authors.initials: J
authors.lastname: Xiang
authors.forename: Limei
authors.initials: L
authors.lastname: Liang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D018797:Cell Cycle Proteins; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics*; D004268:DNA-Binding Proteins; D015536:Down-Regulation / Q000187:drug effects; D004527:Egg Proteins; D053595:Embryonic Stem Cells / Q000166:cytology*; D005854:Germ Cells / Q000166:cytology*; D055429:Growth Differentiation Factor 9; D008562:Membrane Glycoproteins; D051379:Mice; D041681:NIH 3T3 Cells; D009687:Nuclear Proteins; D050814:Octamer Transcription Factor-3; D009828:Oleanolic Acid / Q000494:pharmacology*; D011956:Receptors, Cell Surface; D014158:Transcription, Genetic / Q000187:drug effects; D014212:Tretinoin / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects; D000072216:Zona Pellucida Glycoproteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D004527:Egg Proteins; D055429:Growth Differentiation Factor 9; D008562:Membrane Glycoproteins; D009687:Nuclear Proteins; D050814:Octamer Transcription Factor-3; D011956:Receptors, Cell Surface; C102019:Stra8 protein, mouse; C490062:Sycp3 protein, mouse; D000072216:Zona Pellucida Glycoproteins; C000606519:Zp1 protein, mouse; C000606520:Zp2 protein, mouse; C000606521:Zp3 protein, mouse; D014212:Tretinoin; D009828:Oleanolic Acid