title: High plating efficiency with plant cell cultures.
abstract: In this paper we describe a simple method to improve the plating efficiency in plant cell cultures.Two-stage plating is used; in the first stage the cells are inoculated at high density in 0.2% agarized culture medium for ten days to facilitate growth; under this condition, each cell produces a single micro-colony trapped in the agar network. In the second stage the colonies are plated at different densities in 1% agarized medium.These colonies are self-sufficient and able to improve the cell growth by conditioning the medium.
authors.affiliation: Dipartimento di Biologia Vegetale, Universitâ "La Sapienza", I-00165, Roma, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Bellincampi
authors.forename: N
authors.initials: N
authors.lastname: Baduri
authors.forename: G
authors.initials: G
authors.lastname: Morpurgo