title: A polycaprolactone/cuttlefish bone-derived hydroxyapatite composite porous scaffold for bone tissue engineering.
abstract: Cuttlefish bone (CB) is an attractive natural biomaterial source to obtain hydroxyapatite (HAp). In this study, a porous polycaprolactone (PCL) scaffold incorporating CB-derived HAp (CB-HAp) powder was fabricated using the solvent casting and particulate leaching method. The presence of CB-HAp in PCL/CB-HAp scaffold was confirmed by X-ray diffraction (XRD). Scanning electron microscopy (SEM) and porosity analysis showed that the average pore dimension of the fabricated scaffold was approximately 200-300 μm, with ∼85% porosity, and that the compressive modulus increased after addition of CB-HAp powders. In vitro tests such as cell proliferation assay, cytotoxicity analysis, cell attachment observations, and alkaline phosphatase activity assays showed that the PCL/CB-HAp scaffold could improve the proliferation, viability, adherence, and osteoblast differentiation rate of MG-63 cells. When surgically implanted into rabbit calvarial bone defects, consistent with the in vitro results, PCL/CB-HAp scaffold implantation resulted in significantly higher new bone formation than did implantation of PCL alone. These findings suggest that addition of CB-HAp powder to the PCL scaffold can improve cellular response and that the PCL/CB-HAp composite scaffold has great potential for use in bone tissue engineering.
authors.affiliation: Wonkwang Bone Regeneration Research Institute, Wonkwang University, Iksan, 570-749, Republic of Korea; Bonecell Biotech Inc., Dunsan-dong, Seo-gu, Daejeon, 302-830, Republic of Korea.
authors.forename: Beom-Su
authors.initials: BS
authors.lastname: Kim
authors.forename: Sun-Sik
authors.initials: SS
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Lee
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D049832:Decapodiformes / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008297:Male; D008422:Materials Testing / Q000379:methods; D010012:Osteogenesis / Q000187:drug effects*; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D016062:Porosity; D011817:Rabbits; D012886:Skull / Q000293:injuries* / Q000473:pathology; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; C016240:polycaprolactone; D017886:Durapatite
keywords: bone tissue engineering; cuttlefish bone; hydroxyapatite; polycaprolactone; porous scaffold