title: Simultaneous determination of phenylethanoid glycosides and aglycones by capillary zone electrophoresis with running buffer modifier.
abstract: Although the separation efficiency of capillary electrophoresis (CE) is much higher than that of other chromatographic methods, it is sometimes difficult to adequately separate the complex ingredients in biological samples. This article describes how one effective and simple way to develop the separation efficiency in CE is to add some modifiers to the running buffer. The suitable running buffer modifier β-cyclodextrin (β-CD) was explored to fast and completely separate four phenylethanoid glycosides and aglycones (homovanillyl alcohol, hydroxytyrosol, 3,4-dimethoxycinnamic acid, and caffeic acid) in Lamiophlomis rotata (Lr) and Cistanche by capillary zone electrophoresis with ultraviolet (UV) detection. It was found that when β-CD was used as running buffer modifier, a baseline separation of the four analytes could be accomplished in less than 20 min and the detection limits were as low as 10(-3) mg L(-1). Other factors affecting the CE separation, such as working potential, pH value and ionic strength of running buffer, separation voltage, and sample injection time, were investigated extensively. Under the optimal conditions, a successful practical application on the determination of Lr and Cistanche samples confirmed the validity and practicability of this method.
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China.
authors.forename: Shuqing
authors.initials: S
authors.lastname: Dong
authors.affiliation: Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines of the College of Gansu Province, Department of Pharmacy, Gansu College of Traditional Chinese Medicine, Lanzhou 730000, People's Republic of China.
authors.forename: Ruibin
authors.initials: R
authors.lastname: Gao
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines of the College of Gansu Province, Department of Pharmacy, Gansu College of Traditional Chinese Medicine, Lanzhou 730000, People's Republic of China.
authors.forename: Mei
authors.initials: M
authors.lastname: Guo
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, People's Republic of China.
authors.forename: Jingman
authors.initials: J
authors.lastname: Ni
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China. Electronic address: zhaol@licp.cas.cn.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
mesh_terms: D002021:Buffers; D002109:Caffeic Acids / Q000032:analysis; D002934:Cinnamates / Q000032:analysis; D031671:Cistanche / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006027:Glycosides / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D019686:Lamiaceae / Q000737:chemistry*; D057230:Limit of Detection; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000032:analysis; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D002021:Buffers; D002109:Caffeic Acids; D002934:Cinnamates; D006027:Glycosides; D047392:beta-Cyclodextrins; C005975:3,4-dihydroxyphenylethanol; C065784:3-(3,4-dimethoxyphenyl)propenoic acid; C031215:betadex; D010626:Phenylethyl Alcohol; C040048:caffeic acid
keywords: Capillary electrophoresis; Cistanche; Lamiophlomis rotata; Phenylethanoid glycosides and aglycones; β-Cyclodextrin buffer modifier