title: Human-like collagen/hyaluronic acid 3D scaffolds for vascular tissue engineering.
abstract: Following the previous work of media layer and adventitia layer construction for vascular scaffold, we developed a suitable intima layer scaffold for endothelialization using novel human-like collagen/hyaluronic acid composite at different mass ratios of 40/1, 20/1 and 10/1 (HLC to HA) by freeze-drying process. The structure, mechanical strength, degradation and biocompatibility of the vascular HLC/HA scaffold were evaluated. The results showed that the 10/1 HLC/HA composited an optimal scaffold with (1) an interconnected porous network with a pore diameter of 12±2μm and porosity of 89.3%, (2) better mechanical properties with higher stress of 321.7±15kPa and strain of 45.5±0.2% than 40/1, 20/1 and pure HLC scaffolds, (3) only 9% degradation upon immersion in PBS for 45days at 37°C in vitro, and (4) excellent biocompatibility. This study suggests that the 10/1 HLC/HA composite has a broad prospect of application as luminal vascular scaffold in the tissue engineering.
authors.affiliation: Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an, Shaanxi 710069, China; Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an, Shaanxi 710069, China.
authors.forename: Chenhui
authors.initials: C
authors.lastname: Zhu
authors.forename: Daidi
authors.initials: D
authors.lastname: Fan
authors.forename: Yaoyu
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D001808:Blood Vessels / Q000187:drug effects / Q000502:physiology*; D002452:Cell Count; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology*; D005612:Freeze Drying; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D020007:Implants, Experimental; D008422:Materials Testing; D055595:Mechanical Phenomena; D051379:Mice; D056951:Photoelectron Spectroscopy; D012996:Solutions; D040521:Subcutaneous Tissue / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D012996:Solutions; D006820:Hyaluronic Acid; D003094:Collagen; D006821:Hyaluronoglucosaminidase
keywords: Biocompatibility; Human-like collagen; Hyaluronic acid; Vascular scaffold