title: Chitosan-hydroxycinnamic acid conjugates: preparation, antioxidant and antimicrobial activity.
abstract: In this study, the antioxidant and antimicrobial activities of chitosan-caffeic acid, chitosan-ferulic acid, and chitosan-sinapic acid conjugates with different grafting ratios were investigated. The synthesized chitosan-hydroxycinnamic acid conjugates were verified by performing (1)H NMR and differential scanning calorimetry analysis. The antioxidant activities of the conjugates were increased compared to the unmodified chitosan, by 1.79-fold to 5.05-fold (2,2-diphenyl-1-picrylhydrazyl scavenging assay), 2.44-fold to 4.12-fold (hydrogen peroxide scavenging assay), 1.34-fold to 3.35-fold (ABTS(+) radical scavenging assay), and also exhibited an increased reducing power. The conjugates also showed excellent lipid peroxidation inhibition abilities in a linoleic acid emulsion system. The conjugates exhibited antimicrobial activity against 15 clinical isolates, two standard methicillin-resistant Staphylococcus aureus (MRSA) and three standard methicillin-susceptible S. aureus strains, as well as eight foodborne pathogens. Additionally, the conjugates showed no cytotoxic activity towards human Chang liver and mouse macrophage RAW264.7 cells.
authors.affiliation: POSTECH Ocean Science and Technology Institute, POSTECH, Pohang 790-784, Republic of Korea.
authors.forename: Dae-Sung
authors.initials: DS
authors.lastname: Lee
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Woo
authors.forename: Chang-Bum
authors.initials: CB
authors.lastname: Ahn
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Je
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D003373:Coumaric Acids / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D051379:Mice; D013211:Staphylococcus aureus
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D003373:Coumaric Acids; D048271:Chitosan
keywords: Antimicrobial; Antioxidant; Chitosan; Cytocompatibility; Hydroxycinnamic acid