title: [Anti-allergic effects of xuebijing and potential role of heme oxygenase-1 against ovalbumin-induced murine allergic rhinitis model].
abstract: OBJECTIVE
  In this study, we investigated the anti-inflammation effects of Xuebijing in OVA-induced murine allergic rhinitis model. Furthermore, we determined whether heme oxygenase (HO)-1 is required for the protective activity of Xuebijing.
  
  
  METHODS
  Airways of OVA-sensitized mice exposed to OVA challenge developed eosinophilia, mucus hypersecretion and increased cytokine levels. Levels of interleukin IL-4, IL-5, IL-13, and tumor necrosis factor (TNF)-alpha in nasal lavage fluid were measured using enzyme-linked immunosorbent assays (ELISAs). Lung tissue and nasal mucosa sections were stained with Mayer's hematoxylin and eosin for assessment of cell infiltration and mucus production, Immunohistochemistry, Real-time PCR and Western Blot analyses for HO-1 protein expression.
  
  
  RESULTS
  Orally administered Xuebijing significantly inhibited the number of OVA-induced inflammatory cells and IgE production, along with reduced T-helper (Th) 2 cytokine levels, such as IL-4, IL-5 and IL-13, improved the level of IFN-gamma, in nasal lavage fluid. In addition, Xuebijing induced a marked decrease in OVA-induced inflammatory cell infiltration and mucus production in nasal and lung tissues. These effects were correlated with HO-1 mRNA and protein induction.
  
  
  CONCLUSIONS
  Our results indicate that Xuebijing protects against OVA-induced airway inflammation, at least in part, via HO-1 upregulation.
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Devision of Allergic Medicine, First Hospital of Hebei Medical University, Shijiazhuang, 050031, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Song
authors.forename: Yinghao
authors.initials: Y
authors.lastname: Niu
authors.forename: Jiantao
authors.initials: J
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Xue
authors.forename: Xin
authors.initials: X
authors.lastname: Lv
authors.forename: Jianwang
authors.initials: J
authors.lastname: Yang
authors.forename: Baoshan
authors.initials: B
authors.lastname: Wang
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D004802:Eosinophilia / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D007073:Immunoglobulin E / Q000276:immunology; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D018793:Interleukin-13 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D009297:Nasal Mucosa / Q000378:metabolism; D065631:Rhinitis, Allergic; D012221:Rhinitis, Allergic, Perennial / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D018793:Interleukin-13; D015848:Interleukin-5; D008565:Membrane Proteins; D014409:Tumor Necrosis Factor-alpha; C549376:Xuebijing; D015847:Interleukin-4; D007073:Immunoglobulin E; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse