title: Diets high in heat-treated soybean meal reduce the histamine-induced epithelial response in the colon of weaned piglets and increase epithelial catabolism of histamine.
abstract: We examined the influence of dietary fermentable protein (fCP) and fermentable carbohydrates (fCHO) on the colonic epithelial response to histamine in pigs. Thirty-two weaned piglets were fed 4 diets in a 2 × 2 factorial design with low fCP/low fCHO, low fCP/high fCHO, high fCP/low fCHO and high fCP/high fCHO. After 21-23 days, the pigs were killed and tissue from the proximal colon was stimulated with carbachol, histamine, PGE2 or sodium hydrogen sulphide in Ussing chambers. Changes in short-circuit current and tissue conductance were measured. Diamine oxidase, histamine N-methyltransferase, stem cell growth factor receptor, Fc-epsilon receptor I and cystic fibrosis transmembrane conductance regulator gene expression was determined. Activities of diamine oxidase and histamine N-methyltransferase and numbers of colonic mast cells were measured. The change in the short-circuit current in response to histamine was lower (P = 0.002) and tended to be lower for PGE2 (P = 0.053) in high fCP groups compared to low fCP groups, irrespective of fCHO. Additionally, the change in tissue conductance after the application of histamine was lower (P = 0.005) in the high fCP groups. The expression of histamine N-methyltransferase mRNA (P = 0.033) and the activities of diamine oxidase (P = 0.001) and histamine N-methyltransferase (P = 0.006) were higher with high fCP in comparison with low fCP. The expression of mast cell markers, stem cell growth factor receptor (P = 0.005) and Fc-epsilon receptor I (P = 0.049) was higher with high fCP diets compared to diets low in fCP, whereas the mast cell count did not differ between groups. The expression of the cystic fibrosis transmembrane conductance regulator was reduced (P = 0.001) with high fCP diets compared to low fCP diets. The lower epithelial response to histamine and PGE2 and elevated epithelial histamine inactivation suggests an adaptation to high fCP diets.
authors.affiliation: Institute of Animal Nutrition, Department of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany.
authors.forename: Susan
authors.initials: S
authors.lastname: Kröger
authors.forename: Robert
authors.initials: R
authors.lastname: Pieper
authors.forename: Hubert G
authors.initials: HG
authors.lastname: Schwelberger
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Carmen
authors.initials: C
authors.lastname: Villodre Tudela
authors.forename: Jörg R
authors.initials: JR
authors.lastname: Aschenbach
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Van Kessel
authors.forename: Jürgen
authors.initials: J
authors.lastname: Zentek
mesh_terms: D000821:Animal Feed; D000818:Animals; D002712:Chlorides / Q000378:metabolism; D003106:Colon / Q000378:metabolism*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics / Q000378:metabolism; D003605:D-Amino-Acid Oxidase / Q000235:genetics / Q000378:metabolism; D004032:Diet; D004040:Dietary Carbohydrates / Q000378:metabolism; D004044:Dietary Proteins / Q000378:metabolism; D004789:Enzyme Activation; D005260:Female; D015870:Gene Expression; D006632:Histamine / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D062407:Meals; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics / Q000378:metabolism; D017455:Receptors, IgE / Q000235:genetics / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry*; D013552:Swine; D014886:Weaning
chemical_list: D002712:Chlorides; D004040:Dietary Carbohydrates; D004044:Dietary Proteins; D017455:Receptors, IgE; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D006632:Histamine; D003605:D-Amino-Acid Oxidase; D019009:Proto-Oncogene Proteins c-kit