title: Shikimate pathway activity in shake and fermenter cultures of Cinchona succirubra.
abstract: Cell suspension cultures of Cinchona succirubra were cultivated in shake cultures and for the first time in airlift fermenters. Under both conditions L-tryptophan exerts a stimulatory effect on alkaloid formation. In this context the regulatory pattern of some shikimate pathway enzymes was investigated in non-supplemented and tryptophan supplemented Cinchona cell cultures. A remarkable increase of tryptophan decarboxylase (TDC) activity was observed in Cinchona cells under the influence of tryptophan. Apparently, like in some other indole alkaloid producing cell cultures, a high TDC activity is a prerequisite for alkaloid formation. Growth pattern and some enzyme activities of C. succirubra fermenter cultures at controlled and non-regulated pH levels were followed. Optimum growth and alkaloid formation were recorded under non-regulated (normal) pH conditions.
authors.affiliation: Institut für Biochemie der Pflanzen, Akademie der Wissenschaften der DDR, DDR-4020, Halle/Saale.
authors.forename: H P
authors.initials: HP
authors.lastname: Schmauder
authors.forename: D
authors.initials: D
authors.lastname: Gröger
authors.forename: H
authors.initials: H
authors.lastname: Koblitz
authors.forename: D
authors.initials: D
authors.lastname: Koblitz