title: Evaluation of hsp65 nested PCR-restriction analysis (PRA) for diagnosing tuberculosis in a high burden country.
abstract: Current study evaluated the hsp65 Nested PCR Restriction Fragment Length Polymorphism Analysis (hsp65 Nested PCR-PRA) to detect and identify Mycobacterium tuberculosis complex directly in clinical samples for a rapid and specific diagnosis of tuberculosis (TB). hsp65 Nested PCR-PRA was applied directly to 218 clinical samples obtained from 127 patients suspected of TB or another mycobacterial infection from July 2009 to July 2010. The hsp65 Nested PCR-PRA showed 100% sensitivity and 95.0 and 93.1% specificity in comparison with culture and microscopy (acid fast bacillus smear), respectively. hsp65 Nested PCR-PRA was shown to be a fast and reliable assay for diagnosing TB, which may contribute towards a fast diagnosis that could help the selection of appropriate chemotherapeutic and early epidemiological management of the cases which are of paramount importance in a high TB burden country.
authors.affiliation: Post Graduation Program in Biosciences Applied to Pharmacy, Department of Clinical Analyses and Biomedicine, State University of Maringa, 87020-900 Maringá, PR, Brazil.
authors.forename: Sara
authors.initials: S
authors.lastname: Macente
authors.forename: Clarice Queico
authors.initials: CQ
authors.lastname: Fujimura Leite
authors.forename: Adolfo Carlos Barreto
authors.initials: AC
authors.lastname: Santos
authors.forename: Vera Lúcia Dias
authors.initials: VL
authors.lastname: Siqueira
authors.forename: Luzia Neri Cosmo
authors.initials: LN
authors.lastname: Machado
authors.forename: Nadir Rodrigues
authors.initials: NR
authors.lastname: Marcondes
authors.forename: Mario Hiroyuki
authors.initials: MH
authors.lastname: Hirata
authors.forename: Rosário Dominguez Crespo
authors.initials: RD
authors.lastname: Hirata
authors.forename: Rosilene Fressatti
authors.initials: RF
authors.lastname: Cardoso
mesh_terms: D000328:Adult; D001426:Bacterial Proteins / Q000302:isolation & purification*; D018834:Chaperonin 60 / Q000302:isolation & purification*; D017281:Cost of Illness; D005260:Female; D006801:Humans; D008297:Male; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification* / Q000472:pathogenicity; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D014376:Tuberculosis / Q000175:diagnosis* / Q000235:genetics / Q000382:microbiology / Q000473:pathology
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; C074119:heat-shock protein 65, Mycobacterium