title: Artificial genetic sets composed of size-expanded base pairs.
abstract: We describe in this Minireview the synthesis, properties, and applications of artificial genetic sets built from base pairs that are larger than the natural Watson-Crick architecture. Such designed systems are being explored by several research groups to investigate basic chemical questions regarding the functions of the genetic information storage systems and thus of the origin and evolution of life. For example, is the terrestrial DNA structure the only viable one, or can other architectures function as well? Working outside the constraints of purine-pyrimidine geometry provides more chemical flexibility in design, and the added size confers useful properties such as high binding affinity and helix stability as well as fluorescence. These features are useful for the investigation of fundamental biochemical questions as well as in the development of new biotechnological, biomedical, and nanostructural tools and methods.
authors.affiliation: Department of Chemistry, Stanford University, Stanford, CA 94305 (USA).
authors.forename: Malte
authors.initials: M
authors.lastname: Winnacker
authors.forename: Eric T
authors.initials: ET
authors.lastname: Kool
mesh_terms: D000818:Animals; D020029:Base Pairing; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics; D005813:Genes, Synthetic; D006801:Humans; D009690:Nucleic Acid Conformation; D012313:RNA / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics
chemical_list: D012313:RNA; D004247:DNA
keywords: Watson-Crick base pairing; non-natural nucleobases; nucleic acids; replication; synthetic biology