title: Sulphation can enhance the antioxidant activity of polysaccharides produced by Enterobacter cloacae Z0206.
abstract: The protective effects of sulfated polysaccharide derivatives produced by Enterobacter cloacae Z0206 against H₂O₂-induced oxidative damage in RAW264.7 murine macrophages as well as the possible mechanisms governing the protective effects were studied. Sulfated polysaccharides protected RAW264.7 cells from oxidative damage and apoptosis induced by H₂O₂ by protecting the cellular structure; improving the activity of antioxidant enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px); and inhibiting caspase-3 activation and DNA fragmentation. In addition, the sulfated polysaccharides conferred higher levels of protection from H₂O₂-induced oxidative damage in RAW264.7 murine macrophages compared to the native polysaccharide lacking sulfation. These results indicated that sulfated modifications might be an effective approach to enhance the antioxidant activity of polysaccharides produced by E. cloacae Z0206, and the sulfated derivatives of these polysaccharides may act as potent antioxidant agents.
authors.affiliation: Key Laboratory of Molecular Animal Nutrition of Ministry of Education, College of Animal Sciences, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, PR China; School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, PR China.
authors.forename: Mingliang
authors.initials: M
authors.lastname: Jin
authors.forename: Youming
authors.initials: Y
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Huang
authors.forename: Zeqing
authors.initials: Z
authors.lastname: Lu
authors.forename: Yizhen
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D016972:Enterobacter cloacae / Q000737:chemistry* / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D051379:Mice; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D013463:Sulfuric Acid Esters / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D011135:Polysaccharides, Bacterial; D013463:Sulfuric Acid Esters; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D053148:Caspase 3
keywords: Antioxidant; Apoptosis; Enterobacter cloacae; RAW264.7; Sulfated polysaccharides