title: Simplified analysis of glyphosate and aminomethylphosphonic acid in water, vegetation and soil by liquid chromatography-tandem mass spectrometry.
abstract: BACKGROUND
  There is a need for a simple, fast, efficient and sensitive method for analysis of glyphosate and its degradate aminomethylphosphonic acid (AMPA) in diverse matrices such as water, vegetation and soil.
  
  
  RESULTS
  Aqueous extracts from water, vegetation and soil were passed through reverse-phase and cation-exchange columns and directly injected into a tandem mass spectrometer using only a guard column for separation. Extraction efficiencies from the three matrices were >80% for both glyphosate and AMPA. The method reporting levels (MRLs) for glyphosate in water, vegetation and soil were 3.04 µg L(-1) , 0.05 mg kg(-1) and 0.37 mg kg(-1) respectively. AMPA MRLs were 5.06 µg L(-1) for water, 0.08 mg kg(-1) for vegetation and 0.61 mg kg(-1) for soil.
  
  
  CONCLUSIONS
  A validated, simple and efficient liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for routine analysis of glyphosate and AMPA in water, vegetation and soil that uses minimal sample handling and clean-up will facilitate the additional environmental research needed to address the continuing concerns related to increasing glyphosate use.
authors.affiliation: Minnesota Department of Agriculture, St Paul, MN, USA.
authors.forename: LeEtta J
authors.initials: LJ
authors.lastname: Marek
authors.forename: William C
authors.initials: WC
authors.lastname: Koskinen
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring / Q000379:methods*; D005618:Fresh Water / Q000737:chemistry*; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D006540:Herbicides / Q000032:analysis / Q000378:metabolism; D007555:Isoxazoles; D063065:Organophosphonates / Q000032:analysis* / Q000378:metabolism; D010944:Plants / Q000737:chemistry* / Q000378:metabolism; D012987:Soil / Q000737:chemistry*; D053719:Tandem Mass Spectrometry; D013777:Tetrazoles; D000097797:Glyphosate
chemical_list: D005998:Glycine; D006540:Herbicides; D007555:Isoxazoles; D063065:Organophosphonates; D012987:Soil; D013777:Tetrazoles; D000097797:Glyphosate; C418233:2-amino-3-(3-hydrox-5(2-methyl-2H-tetrazol-5-yl)isoxazol-4-yl)propionic acid
keywords: AMPA; LC-MS/MS analysis; glyphosate; soil; vegetation; water