title: Production of extracellular fibers by tobacco leaf epidermal protoplasts.
abstract: Protoplasts prepared from the upper epidermis of tobacco (Nicotiana tabacum L.) leaves produce tubular structures which are highly fluorescent in the presence of Calcofluor white. The presence of Ca(2+) in the incubation medium appears to be necessary for the production of these fibers. A commercial cellulase preparation destroyed these structures and inhibited their production.
authors.affiliation: Department of Plant Pathology, University of Kentucky, 40546, Lexington, KY, USA.
authors.forename: F F
authors.initials: FF
authors.lastname: Fannin
authors.forename: J G
authors.initials: JG
authors.lastname: Shaw