title: Nicotiana chloroplast genome : 7. Expression in E. coli and B. subtilis of tobacco and Chlamydomonas chloroplast DNA sequences coding for the large subunit of RuBP carboxylase.
abstract: RuBPCase, the enzyme responsible for carboxylation and oxidation of RuBP in a wide variety of photosynthetic organisms, is the major protein found in the chloroplast. Here we present the first evidence for direct expression in E. coli and B. subtilis of tobacco and Chlamydomonas ct-DNA sequences coding for the LS of RuBPCase as demonstrated by a simple in situ immunoassay.
authors.affiliation: Department of Biological Sciences, University of Maryland, Baltimore County, 5401 Wilkens Avenue, 21228, Catonsville, MD, USA.
authors.forename: Y S
authors.initials: YS
authors.lastname: Zhu
authors.forename: P S
authors.initials: PS
authors.lastname: Lovett
authors.forename: D M
authors.initials: DM
authors.lastname: Williams
authors.forename: S D
authors.initials: SD
authors.lastname: Kung