title: Comparison of Architect i2000 for determination of Scc with IMX determination of SCC with different methods.
abstract: BACKGROUND
  SCC (squamous cell carcinoma) antigen (SSCA), a tumor marker, is already used for the diagnosis and follow-up of carcinoma of the cervix and lungs. The aim of our study was the determination of SCCA concentration in 96 serum samples using CMIA (chemiluminesecent microparticle immnoassay) on an Architect i2000 (Abbott Diagnostics) and MEIA (microparticle enzyme immnoassay) on an IMx Chemistry Analyzer (Abbott Diagnostics).
  
  
  METHODS
  In our investigation we used a CMIA (chemiluminesecent microparticle immnoassay) Architect i2000 and MEIA (microparticle enzyme immnoassay) Imx Chemistry Analyzer for SCCA determination.
  
  
  RESULTS
  All patients were hospitalized at the Department of Gynecologic Oncology and Department for Oncology at the University Clinics Center of Sarajevo. The normal serum range of SCCA should be < 0.1 microg/L. The quality control, precision and accuracy of the Architect i2000 were assessed. The quality control was done using quality control serums for low (X = 2.17 microg/L), medium (X = 10.23 microg/L), and high (X = 48.99 microg/L) range. The precision for the Architect i2000 has a CV of 3.44% to 4.44%. We established that the main difference between Architect i2000 and IMx was statistically significant at p < 0.05 according to Student's t-test. The correlation coefficient was r = 0.990. The some samples have higher concentrations on the Architect than on the IMx because the sensitivity of the Architect assay is high (< 0.1 microg/L).
  
  
  CONCLUSIONS
  The CMIA Architect technology is an applicable method significant in diagnostic of SCCA.
authors.affiliation: Institute for Clinical Chemistry and Biochemistry, University of Sarajevo Clinics Center, Sarajevo, Bosnia and Herzegovina. serdarevicnafija@yahoo.com
authors.forename: Nafija
authors.initials: N
authors.lastname: Serdarevic
authors.forename: Adina Elena
authors.initials: AE
authors.lastname: Stanciu
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis*; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D049449:Luminescence; D008175:Lung Neoplasms / Q000175:diagnosis*; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015843:Serpins / Q000032:analysis; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis*
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D015843:Serpins; C051072:squamous cell carcinoma-related antigen