title: Nicorandil protects against ischaemia-reperfusion injury in newborn rat kidney.
abstract: Ischaemia-reperfusion injury (IRI) is the predominant cause of acute kidney injury. Nevertheless, the underlying molecular mechanisms are still unclear. The current study investigated the effects of nicorandil on ATP-sensitive potassium (KATP) channels and the potential signal transduction pathway(s) in a rat kidney IRI model and in cultured tubular HK-2 cells subjected to oxygen and glucose deprivation/reoxygenation (OGD/R) injury. The standard procedure for IRI was performed in newborn rat kidneys. Pretreatment with nicorandil (10 mg/kg) 2 h prior to induction of IRI improved renal function, attenuated tubule damage, and prevented apoptosis of tubule cells, infiltration of neutrophils and macrophages, and production of inflammatory cytokines interleukin (IL)-6, IL-17 and tumour necrosis factor-α. Ischaemia-reperfusion-induced reduction of KIR6.2 was restored to normal levels by nicorandil. The activation of the phosphoinositide-3-kinase (PI3K)-Akt-nuclear factor (NF)-κB axis was detected in this rat kidney IRI model, which was blocked by nicorandil. The renoprotection of nicorandil against IRI was abolished by its inhibitor glibenclamide (1 mg/kg). Similar results were obtained in OGD/R-damaged HK-2 cells. Taken together, our findings demonstrated the specific renoprotective role of nicorandil in the newborn rat IRI kidney by decreasing the production of inflammatory cytokines, and restoring the expression of KIR6.2 potentially through the PI3K-Akt-NF-κB axis.
authors.affiliation: Department of Pediatrics, Second Affiliate Hospital of Harbin Medical University, Harbin, PR China.
authors.forename: Yu-Jing
authors.initials: YJ
authors.lastname: Zhang
authors.forename: Ai-Qi
authors.initials: AQ
authors.lastname: Zhang
authors.forename: Xia-Xia
authors.initials: XX
authors.lastname: Zhao
authors.forename: Zhi-Liang
authors.initials: ZL
authors.lastname: Tian
authors.forename: Li
authors.initials: L
authors.lastname: Yao
mesh_terms: D058186:Acute Kidney Injury / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D007249:Inflammation / Q000188:drug therapy / Q000209:etiology; D054086:KATP Channels / Q000187:drug effects / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D020108:Nicorandil / Q000494:pharmacology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D024661:Potassium Channels, Inwardly Rectifying / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy*; D014665:Vasodilator Agents / Q000494:pharmacology*; D000099161:Kcnj11 Channel
chemical_list: D016207:Cytokines; D054086:KATP Channels; D016328:NF-kappa B; D020108:Nicorandil; D019869:Phosphatidylinositol 3-Kinases; D024661:Potassium Channels, Inwardly Rectifying; D051057:Proto-Oncogene Proteins c-akt; D014665:Vasodilator Agents; D000099161:Kcnj11 Channel