title: Performance evaluation of the innovative PAT test, comparison with the common BAP test and influence of interferences on the evaluation of the plasma antioxidant capacity.
abstract: BACKGROUND
  Antioxidants (AOs) represent the main barrier of defense against damaging aggression due to reactive species, in particular by reactive oxygen species (ROS). The plasma AO capacity is a measure of physiological, environmental, and nutritional factors (exposure to ROS and antioxidant supplementation) determining the redox status in humans and can underline the oxidative stress (OS) conditions in the progression/development of many diseases. Moreover, changes in AO plasma content after supplementation may provide information on the absorption and bioavailability of nutritional compounds and efficacy of AO therapy.
  
  
  OBJECTIVE
  The aim of the study was a comparison between the common BAP (Biological Antioxidant Potential) test, used for the evaluation of the antioxidant capacity, and the innovative PAT (Plasma Antioxidant Test) and to assess both the in vitro interferences of phosphates on the iron reduction and the interference of the plasmatic concentration of phosphates in relation to the plasma antioxidant capacity measured with the two methods.
  
  
  METHODS
  Thirty-six apparently healthy volunteers were involved in the study for the comparison of the two methods.
  
  
  RESULTS
  BAP test and PAT performed on 36 plasma samples demonstrated that plasma antioxidant capacity dosage using the BAP test resulted in overestimated levels in relation to plasma phosphate. Increased BAP values due to phosphates correspond to increased differences between BAP and PAT value (correlation coefficient R = 0.812, p = 0.001).
  
  
  CONCLUSIONS
  PAT can be considered an innovative and predictable method for the measure of the antioxidant power of plasma.
authors.affiliation: Università degli Studi di Urbino "Carlo Bo"-Dip. di Scienze Biomolecolari, Sezione di Biochimica Clinica, Italy.
authors.forename: Benedetti
authors.initials: B
authors.lastname: Serena
authors.forename: Mariangela
authors.initials: M
authors.lastname: Primiterra
authors.forename: Simona
authors.initials: S
authors.lastname: Catalani
authors.forename: Annarosa
authors.initials: A
authors.lastname: Finco
authors.forename: Franco
authors.initials: F
authors.lastname: Canestrari
authors.forename: Umberto
authors.initials: U
authors.lastname: Cornelli
mesh_terms: D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000378:metabolism*; D001682:Biological Availability; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D066298:In Vitro Techniques; D007501:Iron / Q000097:blood; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D010710:Phosphates / Q000097:blood; D010949:Plasma; D017382:Reactive Oxygen Species / Q000378:metabolism; D055815:Young Adult
chemical_list: D000975:Antioxidants; D010710:Phosphates; D017382:Reactive Oxygen Species; D007501:Iron