title: Cryptosin - a new cardenolide in tissue culture and intact plants of Cryptolepis buchanani Roem. & Schult.
abstract: A steroidal glycoside of the digoxin type was isolated from fresh leaves and cultured cells of Cryptolepis buchanani Roem & Schult (Asclepiadaceae). Cultures were raised from leaf and shoot. Explants were cultured on MS medium containing 2,4-D, NAA, BA and coconut milk. Metabolically active calluses were proliferated and maintained on Gamborg's B5 medium with 2,4-D and Kn. The new compound, named cryptosin, was characterised by infrared nmr and mass spectroscopy. Cryptosin was also screened for its pharmacological properties and its cardiotonic action was established.
authors.affiliation: Department of Biochemistry, Indian Institute of Science, 560012, Bangalore, India.
authors.forename: R
authors.initials: R
authors.lastname: Venkateswara
authors.forename: K
authors.initials: K
authors.lastname: Sankara Rao
authors.forename: C S
authors.initials: CS
authors.lastname: Vaidyanathan