title: Callus formation from protoplasts of culturedSpinacia oleracea cells.
abstract: Cell suspensions were initiated from plumule derived calli ofSpinacia oleracea. Some of these cell lines could be maintained in culture for at least three years without a reduced growth rate. A high yield of protoplasts was obtained from the cell suspensions. When protoplasts were cultured in Murashige and Skoog medium with naphthaleneacetic acid and 6-benzyladenine, cell wall formation was observed after three days. The cultured protoplasts produced numerous cell-clusters within two weeks. However only protoplasts isolated from suspensions which were in a rapidly dividing phase were able to divide with a high frequency and give rise to callus colonies.
authors.affiliation: Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University, 640, Matsudo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nakagawa
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Oba
authors.forename: N
authors.initials: N
authors.lastname: Ogura
authors.forename: M
authors.initials: M
authors.lastname: Iizuka