title: Nickel nanoparticle decorated graphene for highly selective isolation of polyhistidine-tagged proteins.
abstract: Nickel nanoparticle decorated graphene (GP-Ni) is prepared by one-pot hydrothermal reduction of graphene oxide and nickel cations by hydrazine hydrate in the presence of poly(sodium-p-styrenesulfonate) (PSS). The GP-Ni hybrid is characterized by XRD, TEM, SEM, XPS, Raman and FT-IR spectra, demonstrating the formation of poly-dispersed nickel nanoparticles with an average size of 83 nm attached on the surface of graphene sheets. The GP-Ni hybrid exhibits ferromagnetic behavior with a magnetization saturation of 31.1 emu g(-1) at 10,000 Oersted (Oe). The GP-Ni also possesses favorable stability in aqueous medium and rapid magnetic response to an external magnetic field. These make it a novel magnetic adsorbent for the separation/isolation of His6-tagged recombinant proteins from a complex sample matrix (cell lysate). The targeted protein species is captured onto the surface of the GP-Ni hybrid via specific metal affinity force between polyhistidine groups and nickel nanoparticles. The SDS-PAGE assay indicates highly selective separation of His6-tagged Smt A from cell lysate. The GP-Ni hybrid displays favorable performance on the separation/isolation of His6-tagged recombinant proteins with respect to the commercial NTA-Ni(2+) column.
authors.affiliation: Research Center for Analytical Sciences, College of Sciences, Northeastern University, Box 332, Shenyang 110819, People's Republic of China.
authors.forename: Jia-Wei
authors.initials: JW
authors.lastname: Liu
authors.forename: Ting
authors.initials: T
authors.lastname: Yang
authors.forename: Lin-Yu
authors.initials: LY
authors.lastname: Ma
authors.forename: Xu-Wei
authors.initials: XW
authors.lastname: Chen
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Wang
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D006639:Histidine / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009532:Nickel / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification*; D013052:Spectrometry, X-Ray Emission; D013059:Spectrum Analysis, Raman; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D011993:Recombinant Fusion Proteins; C033223:polyhistidine; D006639:Histidine; D006108:Graphite; D009532:Nickel