title: Cyclobacterium xiamenense sp. nov., isolated from aggregates of Chlorella autotrophica, and emended description of the genus Cyclobacterium.
abstract: A novel Gram-stain-negative, horseshoe-shaped, non-motile bacterium, designated strain KD51(T), forming colonies coloured pink by carotenoid pigments, was isolated from aggregates of the alga Chlorella autotrophica collected from the coastal sea off the city of Xiamen, Fujian Province, China. 16S rRNA gene sequence comparison showed that strain KD51(T) was a member of the genus Cyclobacterium, forming a distinct lineage with Cyclobacterium lianum HY9(T). The 16S rRNA gene sequence similarity between strain KD51(T) and the type strains of species of the genus Cyclobacterium ranged from 92.1 % to 95.2 %. Growth occurred at 4-40 °C (optimum, 28 °C), in the presence of 3-9 % NaCl (optimum, 3-5 %) and at pH 6-10 (optimum, pH 7.5). The dominant fatty acids (>20 %) of strain KD51(T) were iso-C15 : 0 (32.2 %) and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c; 22.2 %). The DNA G+C content was 41.7 mol% and the only respiratory quinone was menaquinone-7. On the basis of phenotypic data and phylogenetic inference, strain KD51(T) represents a novel species of the genus Cyclobacterium, for which the name Cyclobacterium xiamenense sp. nov. is proposed. The type strain is KD51(T) ( = CGMCC 1.12432(T) = KCTC 32253(T)). An emended description of the genus Cyclobacterium is also proposed.
authors.affiliation: Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Zhangran
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Luxi
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, PR China.|Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Lai
authors.affiliation: Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Huajun
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wei
authors.affiliation: Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Xueqian
authors.initials: X
authors.lastname: Lei
authors.affiliation: Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, PR China.|Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Tianling
authors.initials: T
authors.lastname: Zheng
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D002338:Carotenoids / Q000737:chemistry; D002681:China; D002708:Chlorella / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D002338:Carotenoids; C062629:menaquinone 7