title: Bisindole alkaloids with neural anti-inflammatory activity from Gelsemium elegans.
abstract: Three new trace bisindole alkaloids geleganimines A and B (1, 2) and geleganamide (3) were isolated from the aerial parts of Gelsemium elegans. Their structures were elucidated by spectroscopy, particularly from their carbon-proton coupling constants, and electronic circular dichroism. Compounds 1-3 are the first bisindole alkaloids discovered from the genus Gelsemium. Geleganimine B exhibited anti-inflammatory activity indirectly by suppressing lipopolysaccharide-induced pro-inflammatory factors in BV2 microglial cells with an IC50 value of 10.2 μM. These findings confirm the importance of bioactive trace components in medicinal plant research.
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing 100050, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qu
authors.forename: Lei
authors.initials: L
authors.lastname: Fang
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Ren
authors.forename: Yunbao
authors.initials: Y
authors.lastname: Liu
authors.forename: Shi-Shan
authors.initials: SS
authors.lastname: Yu
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Xiu-Qi
authors.initials: XQ
authors.lastname: Bao
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Shuang-Gang
authors.initials: SG
authors.lastname: Ma
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D031450:Gelsemium / Q000737:chemistry*; D006801:Humans; D026121:Indole Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D017628:Microglia / Q000166:cytology / Q000187:drug effects; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D026121:Indole Alkaloids; D008070:Lipopolysaccharides; C587210:geleganamide; C587209:geleganimine A; C587208:geleganimine B