title: A new bioactive diterpene glycoside from Molinaea retusa from the Madagascar dry forest.
abstract: In a continuing collaboration in a search for new antiproliferative compounds in Madagascar as part of an International Cooperative Biodiversity Group (ICBG), an ethanol extract of Molinaea retusa Radlk. (Sapindaceae) was investigated on the basis of its moderate antiproliferative activity against the A2780 human ovarian cancer cell line (IC50 16 microg/mL). One new compound, 2", 3", 4", 6'-de-O-acetylcupacinoside (1, IC50 15.4 microM) and two known compounds, cupacinoside (2, IC50 9.5 microM) and 6-de-O-acetylcupacinoside (3, IC50 10.9 microM), were isolated by bioassay-directed fractionation using liquid-liquid partitioning, column chromatography, and HPLC. Compounds 2 and 3 also had moderate antiplasmodial activities, with IC50 values of 4.0 and 6.4 microM, respectively, against Plasmodium falciparum, Dd2 strain. The structures were determined using spectroscopic methods.
authors.affiliation: Department of Chemistry, Virginia Tech Center for Drug Discovery, Virginia Tech, Blacksburg, Virginia 24061, USA.
authors.forename: Alexander L
authors.initials: AL
authors.lastname: Eaton
authors.forename: Liva
authors.initials: L
authors.lastname: Harinantenaina
authors.forename: Peggy J
authors.initials: PJ
authors.lastname: Brodie
authors.forename: Maria B
authors.initials: MB
authors.lastname: Cassera
authors.forename: Jessica D
authors.initials: JD
authors.lastname: Bowman
authors.forename: Martin W
authors.initials: MW
authors.lastname: Callmander
authors.forename: Richard
authors.initials: R
authors.lastname: Randrianaivo
authors.forename: Roland
authors.initials: R
authors.lastname: Rakotondrajaona
authors.forename: Etienne
authors.initials: E
authors.lastname: Rakotobe
authors.forename: Vincent E
authors.initials: VE
authors.lastname: Rasamison
authors.forename: David G I
authors.initials: DG
authors.lastname: Kingston
mesh_terms: D000962:Antimalarials / Q000302:isolation & purification*; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification*; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000302:isolation & purification*; D004354:Drug Screening Assays, Antitumor; D006027:Glycosides / Q000302:isolation & purification; D006801:Humans; D008270:Madagascar; D029631:Sapindaceae / Q000737:chemistry*
chemical_list: D000962:Antimalarials; D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D006027:Glycosides