title: Acute restraint stress induces an imbalance in the oxidative status of the zebrafish brain.
abstract: The zebrafish (Danio rerio) has become an emergent model organism for translational approaches focused on the neurobiology of stress due to its genetic, neuroanatomical, and histological similarities with mammalian systems. However, despite the increasing number of studies using zebrafish, reports examining the impact of stress on relevant neurochemical parameters are still elementary when compared to studies using rodents. Additionally, it is important to further validate this model organism by comparing its stress response with those described in other species. Here, we evaluated the effects of an acute restraint stress (ARS) protocol on oxidative stress-related parameters in the zebrafish brain. Our data revealed that ARS significantly decreased catalase activity without altering the activity of superoxide dismutase. Oxidative stress was also indicated by increased levels of lipid peroxides. ARS significantly increased the levels of non-protein thiols, although significant changes in total reduced sulfhydryl content were not detected. These results suggest that ARS is an interesting strategy for evaluating the mechanisms underlying the neurochemical basis of the oxidative profile triggered by acute stressors in the zebrafish brain. Furthermore, this protocol may be suitable for screening new compounds with protective properties against oxidative stress, which plays an increasingly important role in many psychiatric disorders.
authors.affiliation: Laboratório de Psicofarmacologia e Comportamento (LAPCOM), Universidade Comunitária da Região de Chapecó, Avenida Senador Attílio Fontana, 591E, 89809-000 Chapecó, SC, Brazil.
authors.forename: Glaucia
authors.initials: G
authors.lastname: Dal Santo
authors.affiliation: Laboratório de Psicofarmacologia e Comportamento (LAPCOM), Universidade Comunitária da Região de Chapecó, Avenida Senador Attílio Fontana, 591E, 89809-000 Chapecó, SC, Brazil.
authors.forename: Greicy M M
authors.initials: GM
authors.lastname: Conterato
authors.affiliation: Programa de Pós-Graduação em Bioexperimentação, Universidade de Passo Fundo, Campus Universitário, Caixa Postal 611, 99001-970 Passo Fundo, RS, Brazil.
authors.forename: Leonardo J G
authors.initials: LJ
authors.lastname: Barcellos
authors.affiliation: Laboratório de Genética e Ecotoxicologia molecular (LAGEM), Universidade Comunitária da Região de Chapecó, Avenida Senador Attílio Fontana, 591E, 89809-000 Chapecó, SC, Brazil; Programa de Pós Graduação em Ciências Ambientais, Universidade Comunitária da Região de Chapecó, Avenida Senador Attílio Fontana, 591E, 89809-000 Chapecó, SC, Brazil; Zebrafish Neuroscience Research Consortium (ZNRC), USA.
authors.forename: Denis B
authors.initials: DB
authors.lastname: Rosemberg
authors.affiliation: Laboratório de Psicofarmacologia e Comportamento (LAPCOM), Universidade Comunitária da Região de Chapecó, Avenida Senador Attílio Fontana, 591E, 89809-000 Chapecó, SC, Brazil; Programa de Pós Graduação em Ciências Ambientais, Universidade Comunitária da Região de Chapecó, Avenida Senador Attílio Fontana, 591E, 89809-000 Chapecó, SC, Brazil; Zebrafish Neuroscience Research Consortium (ZNRC), USA. Electronic address: angelopiato@gmail.com.
authors.forename: Angelo L
authors.initials: AL
authors.lastname: Piato
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D005260:Female; D015227:Lipid Peroxidation; D008297:Male; D023421:Models, Animal; D018384:Oxidative Stress; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D015027:Zebrafish
chemical_list: D013438:Sulfhydryl Compounds; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Acute restraint stress; Antioxidant defenses; Oxidative stress; Zebrafish