title: 16,17-dihydroxycyclooctatin, a new diterpene from Streptomyces sp. LZ35.
abstract: Genome sequence analysis of Streptomyces sp. LZ35 has revealed a large number of secondary metabolite pathways, including a complete gene cluster for the biosynthesis of cyclooctatin. This cluster contains four genes, cotB1-4, located in a 5-kb region. Optimization of fermentation medium for LZ35Δheng (SR107) led to the identification of cyclooctatin (1) and 16,17-dihydroxycyclooctatin (2), a new diterpene. The structures of these substances were elucidated on the basis of 1D-, 2D-NMR, and HRESIMS data. Cytotoxicity against MDA-MB-231 and A549 cell lines was also evaluated. Results demonstrated that gene cluster and pathway analysis are key to guided isolation of new natural products.
authors.affiliation: Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Ji'nan, Shandong, China.
authors.forename: G S
authors.initials: GS
authors.lastname: Zhao
authors.forename: S R
authors.initials: SR
authors.lastname: Li
authors.forename: Y Y
authors.initials: YY
authors.lastname: Wang
authors.forename: H L
authors.initials: HL
authors.lastname: Hao
authors.forename: Y M
authors.initials: YM
authors.lastname: Shen
authors.forename: C H
authors.initials: CH
authors.lastname: Lu
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D005285:Fermentation; D016680:Genome, Bacterial; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D005810:Multigene Family; D013302:Streptomyces / Q000737:chemistry* / Q000235:genetics
chemical_list: C588368:16,17-dihydroxycyclooctatin; D004224:Diterpenes; C078947:cyclooctatin