title: In situ detection of early replication phases of a gemini virus in legume protoplasts.
abstract: Protoplasts of Phaseolus vulgaris L. (Top Crop), infected with bean golden mosaic virus, were isolated and fixed by various methods for in situ hybridization. An iodine-125 labeled probe was made from the replicative form of the virus. The localization and quantitation was done by autoradiography. Cell wall removal lowered the background and allowed a more accurate analysis. RNase was used to eliminate the possibility of hybrids to RNA. The evidence suggests a sequence of virus movements starting from rough endoplasm reticulum, moving to the nuclear membrane, and finally with the highest concentration inside the nucleus.
authors.affiliation: Department of Genetics and Development, University of Illinois, 61801, Urbana, IL, USA.
authors.forename: D M
authors.initials: DM
authors.lastname: Steffensen
authors.forename: H J
authors.initials: HJ
authors.lastname: Wilson
authors.forename: R M
authors.initials: RM
authors.lastname: Goodman