title: Correlative atomic force microscopy and localization-based super-resolution microscopy: revealing labelling and image reconstruction artefacts.
abstract: Hybrid microscopy: A correlative microscopy tool that combines in situ super-resolution fluorescence microscopy based on single-molecule localization and atomic force microscopy is presented. Direct comparison with high- resolution topography allows the authors to improve fluorescence labeling and image analysis in super-resolution imaging.
authors.affiliation: Madrid Institute for Advanced Studies in Nanoscience (IMDEA Nanociencia), C/Faraday 9, Madrid 28049 (Spain).
authors.forename: Aitor
authors.initials: A
authors.lastname: Monserrate
authors.forename: Santiago
authors.initials: S
authors.lastname: Casado
authors.forename: Cristina
authors.initials: C
authors.lastname: Flors
mesh_terms: D016477:Artifacts; D001583:Benzoxazoles / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry; D007091:Image Processing, Computer-Assisted; D018625:Microscopy, Atomic Force / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D011806:Quinolinium Compounds / Q000737:chemistry
chemical_list: D001583:Benzoxazoles; D004279:DNA, Viral; D011806:Quinolinium Compounds; C075296:1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium
keywords: DNA imaging; artefacts; atomic force microscopy; correlative microscopy; single-molecule microscopy