title: Evaluation of a chemiluminescent enzyme-linked immunosorbent assay for the diagnosis of Trypanosoma cruzi infection in a nonendemic setting.
abstract: The disappearance of lytic, protective antibodies (Abs) from the serum of patients with Chagas disease is accepted as a reliable indicator of parasitological cure. The efficiency of a chemiluminescent enzyme-linked immunosorbent assay based on a purified, trypomastigote-derived glycosylphosphatidylinositol-anchored mucin antigen for the serologic detection of lytic Abs against Trypanosoma cruzi was evaluated in a nonendemic setting using a panel of 92 positive and 58 negative human sera. The technique proved to be highly sensitive {100%; 95% confidence interval (CI) = 96-100} and specific (98.3%; 95% CI = 90.7-99.7), with a kappa score of 0.99. Therefore, this assay can be used to detect active T. cruzi infection and to monitor trypanosomicidal treatment.
authors.affiliation: Barcelona Centre for International Health Research, Hospital Clinic.
authors.forename: Luis
authors.initials: L
authors.lastname: Izquierdo
authors.forename: Alexandre Ferreira
authors.initials: AF
authors.lastname: Marques
authors.forename: Montserrat
authors.initials: M
authors.lastname: Gállego
authors.forename: Sílvia
authors.initials: S
authors.lastname: Sanz
authors.forename: Sílvia
authors.initials: S
authors.lastname: Tebar
authors.forename: Cristina
authors.initials: C
authors.lastname: Riera
authors.forename: Llorenç
authors.initials: L
authors.lastname: Quintó
authors.forename: Edelweiss
authors.initials: E
authors.lastname: Aldasoro
authors.forename: Igor C
authors.initials: IC
authors.lastname: Almeida
authors.forename: Joaquim
authors.initials: J
authors.lastname: Gascon
mesh_terms: D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan; D016022:Case-Control Studies; D014355:Chagas Disease / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D017261:Glycosylphosphatidylinositols; D006801:Humans; D049449:Luminescence; D014349:Trypanosoma cruzi / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D017261:Glycosylphosphatidylinositols