title: Bone regeneration in rat cranium critical-size defects induced by Cementum Protein 1 (CEMP1).
abstract: Gene therapy approaches to bone and periodontal tissue engineering are being widely explored. While localized delivery of osteogenic factors like BMPs is attractive for promotion of bone regeneration; method of delivery, dosage and side effects could limit this approach. A novel protein, Cementum Protein 1 (CEMP1), has recently been shown to promote regeneration of periodontal tissues. In order to address the possibility that CEMP1 can be used to regenerate other types of bone, experiments were designed to test the effect of hrCEMP1 in the repair/regeneration of a rat calvaria critical-size defect. Histological and microcomputed tomography (µCT) analyses of the calvaria defect sites treated with CEMP1 showed that after 16 weeks, hrCEMP1 is able to induce 97% regeneration of the defect. Furthermore, the density and characteristics of the new mineralized tissues were normal for bone. This study demonstrates that hrCEMP1 stimulates bone formation and regeneration and has therapeutic potential for the treatment of bone defects and regeneration of mineralized tissues.
authors.affiliation: Laboratorio de Biología Periodontal, Facultad de Odontología, Universidad Nacional Autónoma de México, México.
authors.forename: Janeth
authors.initials: J
authors.lastname: Serrano
authors.forename: Enrique
authors.initials: E
authors.lastname: Romo
authors.forename: Mercedes
authors.initials: M
authors.lastname: Bermúdez
authors.forename: A Sampath
authors.initials: AS
authors.lastname: Narayanan
authors.forename: Margarita
authors.initials: M
authors.lastname: Zeichner-David
authors.forename: Leticia
authors.initials: L
authors.lastname: Santos
authors.forename: Higinio
authors.initials: H
authors.lastname: Arzate
mesh_terms: D000818:Animals; D001857:Bone Matrix / Q000378:metabolism; D001861:Bone Regeneration / Q000187:drug effects* / Q000235:genetics; D002130:Calcium Phosphates / Q000378:metabolism; D003460:Crystallization; D005780:Gelatin / Q000378:metabolism; D015870:Gene Expression; D008297:Male; D018625:Microscopy, Atomic Force; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics; D011506:Proteins / Q000494:pharmacology*; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D012886:Skull / Q000293:injuries / Q000473:pathology / Q000601:surgery; D054457:Tissue Scaffolds; D014945:Wound Healing
chemical_list: C510886:CEMP1 protein, human; D002130:Calcium Phosphates; D011506:Proteins; D011994:Recombinant Proteins; C022045:octacalcium phosphate; D005780:Gelatin