title: A new laboratorial method for the diagnosis of gastrointestinal parasites in dogs.
abstract: In this study, we aimed to introduce a new technique called TF-Test Modified∕Dog for the diagnosis of gastrointestinal parasites in dogs. Fecal samples from 106 dogs were processed by the technique TF-Test Modified∕Dog and the techniques of centrifugation-flotation in zinc sulfate, simple-flotation by saturated solution of sodium chloride, direct microscopy exam and TF-Test Conventional. Sensitivity was higher in the TF-Test Modified∕Dog (98.41%), followed by flotation in saturated zinc sulfate (77.78%), TF-Test Conventional (73.02%), flotation by saturated sodium chloride (55.55%), and direct microscopy exam (30.16%). The diagnostic efficiency varied from 58.49% to 99.06%, with the highest value also obtained by the new proposed technique. Efficiency level of 99.06% with kappa index 0.979 (almost perfect) was obtained with the TF-Test Modified∕Dog. These results represent significant statistical gains (P < 0.05) of 20.63% in sensitivity and 12.27% in efficiency over the best among the other techniques - flotation by saturated zinc sulfate, whose kappa index was 0.738, much lower than that of the TF-Test Modified∕Dog. All techniques presented 100% specificity. In this sense, the high sensitivity of the TF-Test Modified∕Dog makes it suitable for epidemiological surveys of gastrointestinal parasitosis in dogs, zoonoses control and preventive surveillance programs.
authors.forename: Willian Marinho Dourado
authors.initials: WM
authors.lastname: Coelho
authors.forename: Jancarlo Ferreira
authors.initials: JF
authors.lastname: Gomes
authors.forename: Alessandro Francisco Talamini do
authors.initials: AF
authors.lastname: Amarante
authors.forename: Katia Denise Saraiva
authors.initials: KD
authors.lastname: Bresciani
authors.forename: Giuliano
authors.initials: G
authors.lastname: Lumina
authors.forename: Sumie
authors.initials: S
authors.lastname: Koshino-Shimizu
authors.forename: Denise Pereira
authors.initials: DP
authors.lastname: Leme
authors.forename: Alexandre Xavier
authors.initials: AX
authors.lastname: Falcão
mesh_terms: D000818:Animals; D019411:Clinical Laboratory Techniques; D004283:Dog Diseases / Q000175:diagnosis* / Q000469:parasitology*; D004285:Dogs; D005243:Feces / Q000469:parasitology; D007411:Intestinal Diseases, Parasitic / Q000175:diagnosis / Q000662:veterinary*