title: Secreted heat shock protein gp96-Ig: next-generation vaccines for cancer and infectious diseases.
abstract: Over the past decade, our laboratory has developed a secreted heat shock protein (HSP), chaperone gp96, cell-based vaccine that generates effective anti-tumor and anti-infectious immunity in vivo. Gp96-peptide complexes were identified as an extremely efficient stimulator of MHC I-mediated antigen cross-presentation, generating CD8 cytotoxic T-lymphocyte responses detectable in blood, spleen, gut and reproductive tract to femto-molar concentrations of antigen. These studies provided the first evidence that cell-based gp96-Ig-secreting vaccines may serve as a potent modality to induce both systemic and mucosal immunity. This approach takes advantage of the combined adjuvant and antigen delivery capacity of gp96 for the generation of cytotoxic immunity against a wide range of antigens in both anti-vial and anti-cancer vaccination. Here, we review the vaccine design that utilizes the unique property/ability of endoplasmic HSP gp96 to bind antigenic peptides and deliver them to antigen-presenting cells.
authors.affiliation: Department of Microbiology and Immunology, University of Miami Miller School of Medicine, RMSB 3008, 1600 NW 10th Ave, Miami, FL, 33136, USA, nstrbo@med.miami.edu.
authors.forename: Natasa
authors.initials: N
authors.lastname: Strbo
authors.forename: Arlene
authors.initials: A
authors.lastname: Garcia-Soto
authors.forename: Taylor H
authors.initials: TH
authors.lastname: Schreiber
authors.forename: Eckhard R
authors.initials: ER
authors.lastname: Podack
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism; D003140:Communicable Disease Control / Q000379:methods / Q000639:trends; D003141:Communicable Diseases / Q000276:immunology; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000276:immunology* / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology* / Q000378:metabolism; D007167:Immunotherapy; D008562:Membrane Glycoproteins / Q000276:immunology* / Q000378:metabolism; D009092:Mucous Membrane / Q000276:immunology / Q000378:metabolism; D009369:Neoplasms / Q000276:immunology / Q000401:mortality / Q000628:therapy; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D008562:Membrane Glycoproteins; D014614:Vaccines, Synthetic; C053387:endoplasmin