title: CD4⁺ T cell cytokine gene and protein expression in duodenal mucosa of dogs with inflammatory bowel disease.
abstract: Inflammatory bowel disease (IBD) is a common cause of chronic gastrointestinal signs in dogs. In humans, T helper cells have important roles in the pathogenesis of IBD. In contrast, no specific involvement of a distinct T cell subset has been described in canine IBD. The present study evaluated the gene and protein expression of cytokines of T cell subsets in duodenal mucosa from dogs with IBD. Relative quantification of interleukin (IL)-17A, interferon (IFN)-γ, IL-4 and IL-10 mRNA transcription was performed using duodenal mucosa from 27 IBD dogs and 8 controls. Duodenal mucosal IL-17A, IFN-γ and IL-10 protein levels were determined by ELISA in 15 IBD dogs and 8 controls. There was no significant difference in each cytokines mRNA transcription level between groups. There was no significant difference in IL-17A, IFN-γ and IL-10 protein expression levels between groups. Thus, there is no clear evidence for the involvement of distinct Th cytokine in the pathogenesis of canine IBD.
authors.affiliation: Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohta
authors.forename: Kanae
authors.initials: K
authors.lastname: Takada
authors.forename: Yuji
authors.initials: Y
authors.lastname: Sunden
authors.forename: Yu
authors.initials: Y
authors.lastname: Tamura
authors.forename: Tatsuyuki
authors.initials: T
authors.lastname: Osuga
authors.forename: Sue Yee
authors.initials: SY
authors.lastname: Lim
authors.forename: Masahiro
authors.initials: M
authors.lastname: Murakami
authors.forename: Noboru
authors.initials: N
authors.lastname: Sasaki
authors.forename: Bandula Kumara
authors.initials: BK
authors.lastname: Wickramasekara Rajapakshage
authors.forename: Kensuke
authors.initials: K
authors.lastname: Nakamura
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Takiguchi
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D016207:Cytokines / Q000276:immunology; D017931:DNA Primers / Q000235:genetics; D004283:Dog Diseases / Q000276:immunology* / Q000378:metabolism; D004285:Dogs; D004386:Duodenum / Q000276:immunology*; D005786:Gene Expression Regulation / Q000276:immunology*; D015212:Inflammatory Bowel Diseases / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D007413:Intestinal Mucosa / Q000276:immunology*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D018709:Statistics, Nonparametric
chemical_list: D016207:Cytokines; D017931:DNA Primers