title: Role of TRPV1 in susceptibility to PTZ-induced seizure following repeated hyperthermia challenges in neonatal mice.
abstract: This study was designed to investigate the role of experimental febrile seizures in the induction of generalized clonic seizures and the involvement of heat-sensitive channel TRPV1. Pentylenetetrazol-induced clonic seizure was used as the seizure model, and Trpv1 gene knock-out and wild-type C57/BL6 mice were used as experimental subjects. Electroencephalograph and seizure behavior were recorded for the evaluation of the severity of seizures. Increased frequency of the experimental febrile seizures facilitated PTZ-induced generalized clonic seizures. Trpv1 gene deficiency decreased the properties of generalized clonic seizure. The intensity of experimental febrile seizures reduced the threshold to generalized clonic seizure, and Trpv1 gene deficiency decreased the susceptibility to PTZ-induced seizures following early-life hyperthermia challenges in mice.
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China. Electronic address: pbw99@163.com.
authors.forename: Wei-Lin
authors.initials: WL
authors.lastname: Kong
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China. Electronic address: pbw99@tom.com.
authors.forename: Jia-Wei
authors.initials: JW
authors.lastname: Min
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China. Electronic address: pbw99@sina.com.
authors.forename: Yuan-Lin
authors.initials: YL
authors.lastname: Liu
authors.affiliation: Department of Pharmacology and Toxicology, School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY 14214, USA. Electronic address: junxuli@buffalo.edu.
authors.forename: Jun-Xu
authors.initials: JX
authors.lastname: Li
authors.affiliation: Department of Pathophysiology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China. Electronic address: pengbiwen@hotmail.com.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: He
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China. Electronic address: pengbiwen@whu.edu.cn.
authors.forename: Bi-Wen
authors.initials: BW
authors.lastname: Peng
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D003292:Convulsants / Q000633:toxicity*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004569:Electroencephalography; D006979:Hyperthermia, Induced; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010433:Pentylenetetrazole / Q000633:toxicity*; D003294:Seizures, Febrile / Q000139:chemically induced* / Q000235:genetics*; D050916:TRPV Cation Channels / Q000172:deficiency / Q000378:metabolism*; D013997:Time Factors
chemical_list: D003292:Convulsants; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; D010433:Pentylenetetrazole
keywords: EEG; Epilepsy; Repetitive febrile seizures; TRPV1