title: The synthesis of palmitoylcarnitine by etio-chloroplasts of greening barley leaves.
abstract: CoASH, Mg(2+), ATP and (-)-carnitine were found to be essential for the production of palmitoylcarnitine from palmitate by purified barley etio-chloroplasts. It was concluded that long-chain acyl CoA synthetase (palmitoyl CoA synthetase, EC 6.2.1.3) and carnitine long-chain acyl-transferase (carnitine palmitoyltransferase, EC 2.3.1.21) activity were present in the etio-chloroplasts. It is suggested that the long-chain acylcarnitine formed may move more easily through membrane barriers than the long-chain acyl CoA compound. Also or alternatively this enzyme may spare CoA by transferring long-chain acyl groups from long-chain acyl CoA to carnitine.
authors.affiliation: Department of Plant Biology, Newcastle University, NE1 7RU, Newcastle upon Tyne, UK.
authors.forename: D R
authors.initials: DR
authors.lastname: Thomas
authors.forename: M N
authors.initials: MN
authors.lastname: Jalil
authors.forename: R J
authors.initials: RJ
authors.lastname: Cooke
authors.forename: B C
authors.initials: BC
authors.lastname: Yong
authors.forename: A
authors.initials: A
authors.lastname: Ariffin
authors.forename: P H
authors.initials: PH
authors.lastname: McNeil
authors.forename: C
authors.initials: C
authors.lastname: Wood