title: Protective effect of Bifidobacterium pseudocatenulatum CECT7765 against induced bacterial antigen translocation in experimental cirrhosis.
abstract: OBJECTIVE
  Intervention in the gut ecosystem is considered as a potential strategy to treat liver diseases and their complications. We have evaluated the effects of Bifidobacterium pseudocatenulatum CECT7765 on bacterial translocation and the liver status in experimental cirrhosis.
  
  
  METHODS
  Liver damage was induced in Balb/c mice by weight-controlled oral administration of carbon tetrachloride. Laparotomies were performed at week 12. One week prior to laparotomy, animals received B. pseudocatenulatum CECT7765 (10(9) cfu/daily) or placebo intragastrically. All animals received Escherichia coli (10(7) cfu/single dose) intragastrically 24 hours before laparotomy. A group of naïve non-treated animals was included as control. Liver tissue specimens, mesenteric lymph nodes, intestinal content and blood were collected. Liver histology, profibrogenic genes expression, bacterial DNA translocation, serum endotoxaemia and liver cytokine levels were measured.
  
  
  RESULTS
  Bifidobacterium pseudocatenulatum CECT7765 showed no significant effect on structural liver damage, as determined by histological evaluation, alpha-smooth muscle actin distribution, profibrogenic gene expression levels, total hydroxyproline levels and malon dialdehyde production compared with mice receiving placebo. Interestingly, bacterial DNA translocation and serum endotoxin levels were significantly decreased in mice receiving the Bifidobacterium strain compared with placebo. Gut barrier integrity markers were up-regulated in mice receiving B. pseudocatenulatum CECT7765 and quantitatively correlated with intestinal gene copy numbers of the bifidobacterial strain. Gene expression levels of several anti-inflammatory mediators were also increased in mice receiving B. pseudocatenulatum CECT7765 compared with placebo.
  
  
  CONCLUSIONS
  Oral administration of B. pseudocatenulatum CECT7765 is associated with improved gut barrier integrity and shows a beneficial effect against induced bacterial antigen translocation in the CCl4 -model of cirrhosis.
authors.affiliation: CIBERehd, Instituto de Salud Carlos III, Madrid, Spain; Unidad Hepática, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Valencia, Spain.
authors.forename: Alba
authors.initials: A
authors.lastname: Moratalla
authors.forename: Isabel
authors.initials: I
authors.lastname: Gómez-Hurtado
authors.forename: Arlette
authors.initials: A
authors.lastname: Santacruz
authors.forename: Ángela
authors.initials: Á
authors.lastname: Moya
authors.forename: Gloria
authors.initials: G
authors.lastname: Peiró
authors.forename: Pedro
authors.initials: P
authors.lastname: Zapater
authors.forename: José M
authors.initials: JM
authors.lastname: González-Navajas
authors.forename: Paula
authors.initials: P
authors.lastname: Giménez
authors.forename: José
authors.initials: J
authors.lastname: Such
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Sanz
authors.forename: Rubén
authors.initials: R
authors.lastname: Francés
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D018988:Bacterial Translocation; D001644:Bifidobacterium / Q000502:physiology*; D002251:Carbon Tetrachloride; D004269:DNA, Bacterial / Q000235:genetics; D004731:Endotoxins / Q000097:blood; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology*; D005260:Female; D007422:Intestines / Q000382:microbiology*; D008099:Liver / Q000382:microbiology* / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000382:microbiology / Q000473:pathology / Q000628:therapy*; D008807:Mice, Inbred BALB C; D019936:Probiotics
chemical_list: D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D004731:Endotoxins; D002251:Carbon Tetrachloride
keywords: B. pseudocatenulatum CECT7765; bacterial translocation; cirrhosis; inflammation; microbiota