title: Correlation between the presence of membrane-bound auxin binding and root regeneration in cultured tobacco cells.
abstract: When cell-suspension cultures and callus tissue from Nicotiana tabacum are grown on medium containing α-naphthaleneacetic acid (NAA) and kinetin, three classes of auxin-binding proteins can be detected. When the herbicide 2,4-dichlorophenoxyacetic acid is used instead of both NAA and kinetin, one of these sites, which is membranebound, disappears. After retransferring cells to medium containing NAA and kinetin, this membrane-bound site reappears after four to eight weeks. This reappearance is correlated with the ability of the cells to regenerate roots.
authors.affiliation: Chicago Medical School, University of Health Sciences, 3333 Green Bay Road, 60064, North Chicago, Il, USA.
authors.forename: A C
authors.initials: AC
authors.lastname: Maan
authors.forename: P C
authors.initials: PC
authors.lastname: van der Linde
authors.forename: P A
authors.initials: PA
authors.lastname: Harkes
authors.forename: K R
authors.initials: KR
authors.lastname: Libbenga