title: Novel hybrid structure silica/CdTe/molecularly imprinted polymer: synthesis, specific recognition, and quantitative fluorescence detection of bovine hemoglobin.
abstract: This work presented a novel strategy for the synthesis of the hybrid structure silica/CdTe/molecularly imprinted polymer (Si-NP/CdTe/MIP) to recognize and detect the template bovine hemoglobin (BHb). First, amino-functionalized silica nanoparticles (Si-NP) and carboxyl-terminated CdTe quantum dots (QDs) were assembled into composite nanoparticles (Si-NP/CdTe) using the EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) chemistry. Next, Si-NP/CdTe/MIP was synthesized by anchoring molecularly imprinted polymer (MIP) layer on the surface of Si-NP/CdTe through the sol-gel technique and surface imprinting technique. The hybrid structure possessed the selectivity of molecular imprinting technique and the sensitivity of CdTe QDs as well as well-defined morphology. The binding experiment and fluorescence method demonstrated its special recognition performance toward the template BHb. Under the optimized conditions, the fluorescence intensity of the Si-NP/CdTe/MIP decreased linearly with the increase of BHb in the concentration range 0.02-2.1 μM, and the detection limit was 9.4 nM. Moreover, the reusability and reproducibility and the successful applications in practical samples indicated the synthesis of Si-NP/CdTe/MIP provided an alternative solution for special recognition and determination of protein from real samples.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, and Department of Chemistry, Nankai University , Tianjin 300071, People's Republic of China.
authors.forename: Dong-Yan
authors.initials: DY
authors.lastname: Li
authors.forename: Xi-Wen
authors.initials: XW
authors.lastname: He
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.forename: Wen-You
authors.initials: WY
authors.lastname: Li
authors.forename: Yu-Kui
authors.initials: YK
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D000818:Animals; D019187:Cadmium Compounds / Q000737:chemistry*; D002417:Cattle; D006454:Hemoglobins / Q000032:analysis*; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013691:Tellurium / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D006454:Hemoglobins; D011108:Polymers; D012822:Silicon Dioxide; D013691:Tellurium; C028337:cadmium telluride