title: Somatic embryogenesis from mesophyll protoplasts of Trigonella corniculata (leguminosae).
abstract: Mesophyll protoplasts isolated enzymatically from Trigonella corniculata divided to form callus, with a plating efficiency of 49% in Kao (1977) medium. Protoplast-derived tissues formed somatic embryoids at high frequency on MS medium with 2.0 mg L(-7) NAA and 0.5 mg L(-7) BAP. Embryoids developed into plants on MS medium lacking hormones.
authors.affiliation: Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, Nottingham, UK.
authors.forename: D Y
authors.initials: DY
authors.lastname: Lu
authors.forename: M R
authors.initials: MR
authors.lastname: Davey
authors.forename: E C
authors.initials: EC
authors.lastname: Cocking