title: Studies on Ailanthus altissima cell suspension cultures : Precursor feeding of L-[methylene-(14)C]tryptophan and L-tryptophan.
abstract: The uptake of L-[methylene (14)C]-tryptophan from culture medium and the subsequent incorporation of the radiolabel into canthin-6-one, 1-hydroxycanthin-6-one and 1-methoxycanthin-6-one has been demonstrated in cell suspension cultures of Ailanthus altissima. Efficient incorporation has been shown to depend significantly on the time of feeding. Furthermore, feeding of L-tryptophan, at levels of 500 mg/l resulted in improved alkaloid yields, particularly when fed during the lag phase of the growth cycle.
authors.affiliation: Department of Pharmacognosy, The School of Pharmacy, 29-39 Brunswick Square, WC1N 1AX, London, UK.
authors.forename: L A
authors.initials: LA
authors.lastname: Anderson
authors.forename: C A
authors.initials: CA
authors.lastname: Hay
authors.forename: M F
authors.initials: MF
authors.lastname: Roberts
authors.forename: J D
authors.initials: JD
authors.lastname: Phillipson