title: Sequence-based analysis of translocations and inversions in bread wheat (Triticum aestivum L.).
abstract: Structural changes of chromosomes are a primary mechanism of genome rearrangement over the course of evolution and detailed knowledge of such changes in a given species and its close relatives should increase the efficiency and precision of chromosome engineering in crop improvement. We have identified sequences bordering each of the main translocation and inversion breakpoints on chromosomes 4A, 5A and 7B of the modern bread wheat genome. The locations of these breakpoints allow, for the first time, a detailed description of the evolutionary origins of these chromosomes at the gene level. Results from this study also demonstrate that, although the strategy of exploiting sorted chromosome arms has dramatically simplified the efforts of wheat genome sequencing, simultaneous analysis of sequences from homoeologous and non-homoeologous chromosomes is essential in understanding the origins of DNA sequences in polyploid species.
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO) Plant Industry, St Lucia, Brisbane, Queensland, Australia ; Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ma
authors.forename: Jiri
authors.initials: J
authors.lastname: Stiller
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Berkman
authors.forename: Yuming
authors.initials: Y
authors.lastname: Wei
authors.forename: Jan
authors.initials: J
authors.lastname: Rogers
authors.forename: Catherine
authors.initials: C
authors.lastname: Feuillet
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Dolezel
authors.forename: Klaus F
authors.initials: KF
authors.lastname: Mayer
authors.forename: Kellye
authors.initials: K
authors.lastname: Eversole
authors.forename: You-Liang
authors.initials: YL
authors.lastname: Zheng
authors.forename: Chunji
authors.initials: C
authors.lastname: Liu
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics; D014178:Translocation, Genetic / Q000235:genetics; D014908:Triticum / Q000235:genetics*