title: Peroxisomal ATP-binding cassette transporter COMATOSE and the multifunctional protein abnormal INFLORESCENCE MERISTEM are required for the production of benzoylated metabolites in Arabidopsis seeds.
abstract: Secondary metabolites derived from benzoic acid (BA) are of central importance in the interactions of plants with pests, pathogens, and symbionts and are potentially important in plant development. Peroxisomal β-oxidation has recently been shown to contribute to BA biosynthesis in plants, but not all of the enzymes involved have been defined. In this report, we demonstrate that the peroxisomal ATP-binding cassette transporter COMATOSE is required for the accumulation of benzoylated secondary metabolites in Arabidopsis (Arabidopsis thaliana) seeds, including benzoylated glucosinolates and substituted hydroxybenzoylcholines. The ABNORMAL INFLORESCENCE MERISTEM protein, one of two multifunctional proteins encoded by Arabidopsis, is essential for the accumulation of these compounds, and MULTIFUNCTIONAL PROTEIN2 contributes to the synthesis of substituted hydroxybenzoylcholines. Of the two major 3-ketoacyl coenzyme A thiolases, KAT2 plays the primary role in BA synthesis. Thus, BA biosynthesis in Arabidopsis employs the same core set of β-oxidation enzymes as in the synthesis of indole-3-acetic acid from indole-3-butyric acid.
authors.affiliation: Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia.
authors.forename: John D
authors.initials: JD
authors.lastname: Bussell
authors.forename: Michael
authors.initials: M
authors.lastname: Reichelt
authors.forename: Andrew A G
authors.initials: AA
authors.lastname: Wiszniewski
authors.forename: Jonathan
authors.initials: J
authors.lastname: Gershenzon
authors.forename: Steven M
authors.initials: SM
authors.lastname: Smith
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000251:Adenosine Triphosphatases; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D019817:Benzoic Acid / Q000378:metabolism*; D001588:Benzoylcholine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D002794:Choline / Q000737:chemistry / Q000378:metabolism; D002934:Cinnamates / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005961:Glucosinolates / Q000378:metabolism; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010084:Oxidation-Reduction; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism; D064210:Secondary Metabolism; D012639:Seeds / Q000235:genetics / Q000378:metabolism*
chemical_list: C401414:AIM1 protein, Arabidopsis; D018528:ATP-Binding Cassette Transporters; D029681:Arabidopsis Proteins; D002934:Cinnamates; D005961:Glucosinolates; C428126:KAT2 protein, Arabidopsis; D009097:Multienzyme Complexes; D024642:Potassium Channels, Voltage-Gated; C029010:cinnamic acid; D001588:Benzoylcholine; C039127:4-hydroxybenzoylcholine; D019817:Benzoic Acid; D000251:Adenosine Triphosphatases; C456843:At4g39850 protein, Arabidopsis; D002794:Choline; D020156:Salicylic Acid