title: Segregation of T-DNA copies in the progeny of a regenerant plant from a mannopine-positive hairy root line.
abstract: We have analyzed opine content, T-DNA content, and developmental features of one hairy root culture line and its progeny. Opine-positive progeny still have the hairy root phenotype and have essentially the same T-DNA structure as the parental plant. Opineless progeny do not exhibit the hairy root phenotype. Some of them do not contain any T-DNA sequences, whereas others contain only the left part of T-DNA. These results are compatible with the hypothesis that, in the hairy root line analyzed, the left part of T-DNA is integrated independently from the core T-DNA and can therefore segregate during sexual reproduction.
authors.affiliation: Groupe de recherche sur les interactions entre microorganismes et plantes, Génétique et amélioration des plantes, INRA, Bat.409, F-91405, Orsay Cedex 05, France.
authors.forename: C
authors.initials: C
authors.lastname: David
authors.forename: J
authors.initials: J
authors.lastname: Tempé