title: Bone healing with oxytocin-loaded microporous β-TCP bone substitute in ectopic bone formation model and critical-sized osseous defect of rat.
abstract: OBJECTIVE
  This study investigated the efficacy of the hypothalamic nonapeptide oxytocin (OT) by direct delivery to local defects using a microporous β-tricalcium phosphate (TCP) as the carrier for the future applications as a method to achieve predictable bone regeneration of large osseous defects requiring sinus bone graft and guided bone regeneration procedures for implant placement.
  
  
  METHODS
  Both the ectopic and new bone formation induced by the OT-loaded microporous β-TCP powder was histomorphometrically compared with unloaded β-TCP in a subcutaneous ectopic bone formation model and calvarial critical-sized defects (CSDs) in 45 rats.
  
  
  RESULTS
  The OT-loaded β-TCP clearly enhanced ectopic bone formation compared with the unloaded control group. A High initial OT dose (250 μg) significantly increased ectopic bone formation at an early healing time-point compared with a lower OT dose (50 μg). The OT-loaded samples displayed greater new bone formation in the rat calvarial CSDs. Extensive new bone formation was achieved in the calvarial CSDs with the higher OT dose.
  
  
  CONCLUSIONS
  These results suggest that local OT delivery to bone substitute promotes new bone formation via an osteoinductive mode of action.
authors.affiliation: Department of Periodontology, School of Dentistry, Kyungpook National University, Daegu, Korea.
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Park
authors.forename: Jae-Min
authors.initials: JM
authors.lastname: Kim
authors.forename: Heon-Jin
authors.initials: HJ
authors.lastname: Lee
authors.forename: Seong-Hwa
authors.initials: SH
authors.lastname: Jeong
authors.forename: Jo-Young
authors.initials: JY
authors.lastname: Suh
authors.forename: Takao
authors.initials: T
authors.lastname: Hanawa
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001847:Bone Diseases / Q000473:pathology / Q000601:surgery*; D001861:Bone Regeneration / Q000187:drug effects; D002130:Calcium Phosphates / Q000737:chemistry*; D004058:Diffusion; D004195:Disease Models, Animal; D004337:Drug Carriers; D048091:Guided Tissue Regeneration / Q000379:methods; D008297:Male; D009999:Ossification, Heterotopic / Q000139:chemically induced* / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects; D010121:Oxytocin / Q000494:pharmacology / Q000627:therapeutic use*; D010316:Particle Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000187:drug effects / Q000473:pathology / Q000601:surgery*; D040521:Subcutaneous Tissue / Q000187:drug effects / Q000473:pathology; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D004337:Drug Carriers; C485817:beta-tricalcium phosphate; D010121:Oxytocin
keywords: bone graft; bone regeneration; ectopic bone formation; histomorphometry; oxytocin