title: Participation of an active transport system in berberine-secreting cultured cells of Thalictrum minus.
abstract: The release of the benzylisoquinoline alkaloid berberine from cultured cells of Thalictrum minus into the medium proved to be temperature-dependent and was suppressed by such inhibitors of the plasma membrane-bound ATPase as vanadate and diethylstilbestrol. These results indicate that berberine is secreted through an energy-requiring process located in the plasma membrane of berberine-producing T. minus cells. This is the first finding that a secondary metabolite of plant cell culture is secreted by an active transport system.
authors.affiliation: Faculty of Pharmaceutical Sciences, Kyoto University, Yoshida, Sakyo-ku, 606, Kyoto, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yamamoto
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Suga
authors.forename: H
authors.initials: H
authors.lastname: Fukui
authors.forename: M
authors.initials: M
authors.lastname: Tabata