title: Vitamin C enhances bacterial cellulose production in Gluconacetobacter xylinus.
abstract: Influence of vitamin C (ascorbic acid) on bacterial cellulose (BC) production and crystal structure was studied using four strains of Gluconacetobacter xylinus (ATCC 10245, IFO 13693, 13772 and 13773). BC productivity of all strains was increased in presence of vitamin C (0.5% w/w), the average BC production reached 0.47 g/30 ml compared with 0.25 g/30 ml without vitamin C. Enhanced productivity is associated with a decrease in gluconic acid concentration that is produced from Gluconacetobacter xylinus during BC production. X-ray results showed that the crystallinity index of BC produced in presence of ascorbic acid was the lowest with remarkable change in d-spacing. These results were confirmed by using solid state (13)CNMR. The increase in BC yield in presence of vitamin C is due to its antioxidant behavior and confirms our past work on lignosulfonate influence on BC.
authors.affiliation: King Khalid University, Faculty of Science, Chemistry Department, P.O. Box 9004, Abha 61413, Saudi Arabia; Ain-Shams University, Institute of Environmental Studies and Research, Basic Science Department, Abbassia, Cairo 11566, Egypt. Electronic address: keshksherif@gmail.com.
authors.forename: Sherif M A S
authors.initials: SM
authors.lastname: Keshk
mesh_terms: D001205:Ascorbic Acid / Q000378:metabolism / Q000494:pharmacology*; D002482:Cellulose / Q000096:biosynthesis* / Q000737:chemistry; D003460:Crystallization; D003470:Culture Media; D005285:Fermentation; D041822:Gluconacetobacter xylinus / Q000187:drug effects / Q000378:metabolism*; D005942:Gluconates / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003470:Culture Media; D005942:Gluconates; D011135:Polysaccharides, Bacterial; D002482:Cellulose; D001205:Ascorbic Acid; C030691:gluconic acid
keywords: Bacterial cellulose; Gluconacetobacter xylinus; Solid state (13)CNMR; Vitamin C; X-ray diffractogram