title: Enzyme evolution beyond gene duplication: A model for incorporating horizontal gene transfer.
abstract: Understanding the evolution of enzyme function after gene duplication has been a major goal of molecular biologists, biochemists and evolutionary biologists alike, for almost half a century. In contrast, the impact that horizontal gene transfer (HGT) has had on the evolution of enzyme specialization and the assembly of metabolic networks has just started to being investigated. Traditionally, evolutionary studies of enzymes have been limited to either the function of enzymes in vitro, or to sequence variability at the population level, where in almost all cases the starting conceptual framework embraces gene duplication as the mechanism responsible for the appearance of genetic redundancy. Very recently, we merged comparative phylogenomics, detection of selection signals, enzyme kinetics, X-ray crystallography and computational molecular dynamics, to characterize the sub-functionalization process of an amino acid biosynthetic enzyme prompted by an episode of HGT in bacteria. Some of the evolutionary implications of these functional studies, including a proposed model of enzyme specialization independent of gene duplication, are developed in this commentary.
authors.affiliation: Evolution of Metabolic Diversity Laboratory; Laboratorio Nacional de Genómica para la Biodiversidad (Langebio); Cinvestav-IPN; Irapuato, México.
authors.forename: Lianet
authors.initials: L
authors.lastname: Noda-García
authors.forename: Francisco
authors.initials: F
authors.lastname: Barona-Gómez
keywords: enzyme evolution; horizontal gene transfer; subHisA and PriA; substrate specificity; tryptophan and histidine biosynthesis