title: On the importance of crystallographic texture in the biocompatibility of titanium based substrate.
abstract: The role of grain size and crystallographic orientation on the biocompatibility of commercially pure titanium was investigated. Samples, with significant differences in crystallographic texture and average grain size (from 0.4 to 40 µm) were produced by equal channel angular pressing (ECAP) and post deformation annealing. X-ray diffraction and electron back scattered diffraction (EBSD) were used to evaluate differences in texture and microstructural characteristics. The titanium oxide film present on the surface of the samples was analyzed to determine the oxidation state of titanium and the chemical bonds between oxygen and titanium using X-ray photoelectron spectroscopy (XPS). Biocompatibility experiments were conducted using MC3T3 preosteoblast cells. Cell attachment was found to be texture-sensitive, where the number of attached cells was higher on the samples with higher number of (0002) planes exposed to the surface, regardless of the grain size. A relationship was also found between the titanium oxide species formed on the surface and the crystallographic texture underneath. The surface texture consisting of more densely packed basal planes promote the formation of Ti-OH on the surface, which in turn, enhances the cell-substrate interactions. These surface characteristics are deemed responsible for the observed difference in cell attachment behaviour of surfaces with different textures. Finally, it is inferred that texture, rather than the grain size, plays the major role in controlling the surface biocompatibility of biomedical devices fabricated from pure metallic titanium.
authors.affiliation: École de Technologie Supérieure, Montréal, Quebec, Canada.
authors.forename: Majid
authors.initials: M
authors.lastname: Hoseini
authors.forename: Philippe
authors.initials: P
authors.lastname: Bocher
authors.forename: Arash
authors.initials: A
authors.lastname: Shahryari
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Azari
authors.forename: Jerzy A
authors.initials: JA
authors.lastname: Szpunar
authors.forename: Hojatollah
authors.initials: H
authors.lastname: Vali
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D003461:Crystallography; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D056951:Photoelectron Spectroscopy; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D014025:Titanium
keywords: ECAP; biocompatibility; commercially pure titanium; crystallographic texture; grain size