title: GC/GC-MS analysis, isolation and identification of bioactive essential oil components from the Bhutanese medicinal plant, Pleurospermum amabile.
abstract: We have hydrodistilled the essential oil (EO) from the aerial parts of the Bhutanese medicinal plant, Pleurospermum amabile using a Clevenger apparatus and evaluated this EO by GC/GC-MS and NMR analysis followed by testing for bioactivity. The GC-MS analysis identified 52 compounds with (E)-isomyristicin as a major component (32.2%). Repeated purification yielded four compounds; (E)-isomyristicin (1), (E)-isoapiol (2), methyl eugenol (3) and (E)-isoelemicin (4). Compound 2 and the mother EO showed the best antiplasmodial activity against the Plasmodium falciparum strains, TM4/8.2 (chloroquine and antifolate sensitive) and K1CB1 (multidrug resistant). They exhibited mild antibacterial activity against Bacillus subtilis. None of the test samples showed cytotoxicity.
authors.affiliation: School of Chemistry, University of Wollongong, Wollongong, NSW 2522, Australia.
authors.forename: Phurpa
authors.initials: P
authors.lastname: Wangchuk
authors.forename: Paul A
authors.initials: PA
authors.lastname: Keller
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Pyne
authors.forename: Malai
authors.initials: M
authors.lastname: Taweechotipatr
authors.forename: Sumalee
authors.initials: S
authors.lastname: Kamchonwongpaisan
mesh_terms: D000081248:Allylbenzene Derivatives; D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D019661:Apiaceae / Q000737:chemistry*; D001593:Benzyl Compounds / Q000302:isolation & purification*; D001631:Bhutan; D002522:Chlorocebus aethiops; D004148:Dioxolanes / Q000302:isolation & purification*; D004354:Drug Screening Assays, Antitumor; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007624:KB Cells; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry; D011748:Pyrogallol / Q000031:analogs & derivatives* / Q000302:isolation & purification; D014709:Vero Cells
chemical_list: D000081248:Allylbenzene Derivatives; D000900:Anti-Bacterial Agents; D001593:Benzyl Compounds; D004148:Dioxolanes; D009822:Oils, Volatile; D011748:Pyrogallol; C005246:myristicin