title: Silencing MAP3K1 expression through RNA interference enhances paclitaxel-induced cell cycle arrest in human breast cancer cells.
abstract: The objective of this study is to compare the expression level of MAP3K1 between normal mammary gland cells and breast cancer cells, and to analyze the effects of silencing MAP3K1 on breast cancer cells with paclitaxel treatment. Western blotting analysis was used to detect the expression level of MAP3K1 in MCF-7 and MCF-12F cells. The effect of gene silencing through different siRNAs was determined by realtime-PCR. MTT assay was used to test the cell proliferation. Cell cycle was detected by flow cytometry. MAP3K1 protein expression level in breast cancer cells was higher than that in normal mammary gland cells. MAP3K1 siRNA transfection significantly reduced the expression level of MAP3K1, and enhanced paclitaxel-induced cell proliferation inhibition and cell cycle arrest in breast cancer cells. Targeting MAP3K1 expression through small RNA interference can promote the therapeutic effects of paclitaxel in breast cancer.
authors.affiliation: Jiangxi Breast Center Third Hospital of Nanchang, Nanchang, 33009, China.
authors.forename: Pinghua
authors.initials: P
authors.lastname: Hu
authors.forename: Qin
authors.initials: Q
authors.lastname: Huang
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Li
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Wu
authors.forename: Qianwen
authors.initials: Q
authors.lastname: Ouyang
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Yali
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001943:Breast Neoplasms; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D049109:Cell Proliferation; D056744:Cyclin B1 / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006801:Humans; D048728:MAP Kinase Kinase Kinase 1 / Q000235:genetics* / Q000378:metabolism; D061986:MCF-7 Cells; D042361:Mammary Glands, Human / Q000201:enzymology / Q000473:pathology; D017239:Paclitaxel / Q000494:pharmacology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D020687:cdc25 Phosphatases / Q000235:genetics / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C534699:CCNB1 protein, human; D056744:Cyclin B1; D034741:RNA, Small Interfering; D048728:MAP Kinase Kinase Kinase 1; C482576:MAP3K1 protein, human; C517009:CDC25C protein, human; D020687:cdc25 Phosphatases; D017239:Paclitaxel