title: Integral association of phytochrome with a membranous fraction from etiolatedAvena shoots: red/far-red photoreversibility and in vitro characterization.
abstract: The results reported in this paper provide strong evidence to support the belief that the small percentage of phytochrome recovered in low-speed centrifugation pellets, when prepared in the absence of divalent cations after various in vivo irradiations, is not simply a manifestation of non-specific co-precipitation of soluble phytochrome.The far-red reversibility of the observed near-doubling of phytochrome pelletability after in vivo red irradiation indicates that phytochrome pelletability in the absence of divalent cations is a phytochrome-controlled response. The characteristics of the pelleted phytochrome indicate a strong, hydrophobic interaction with membranes. A tentative proposal to explain the observed characteristics of the association of phytochrome with membranous material in the absence of divalent cations after different in vivo irradiations has been put forward.
authors.affiliation: Department of Botany, University of Leicester, LE1 7RH, Leicester, UK.
authors.forename: P J
authors.initials: PJ
authors.lastname: Watson
authors.forename: H
authors.initials: H
authors.lastname: Smith