title: A colony hybridization study of mRNA in maturating soybean seeds.
abstract: Polysomal poly(A)RNA was transcribed by avian myeloblastosis virus (AMV) reverse transcriptase and double-stranded complementary DNA (cDNA) was then synthesized and cloned in Escherichia coli C600 by (dG)-(dC) tailing in the PstI site of pBR322. A bank of mRNA sequences from maturating soybean seeds (average weight 200 mg) was obtained. (32)P-cDNA based on mRNA from various organs of the plant (leaves, axes, seeds) was synthesized. The clones were hybridized with different kinds of (32)P-cDNA on nitrocellulose filters. Approximately 13% of the clones were specific for the maturating stage, and more than a half the clones gave a positive result with all kinds of cDNA.
authors.affiliation: Institute of Genetics and Selection of Industrial Microorganisms, Dorozhnaya 8, 113545, Moscow, USSR.
authors.forename: S M
authors.initials: SM
authors.lastname: Epishin
authors.forename: S L
authors.initials: SL
authors.lastname: Kiselev
authors.forename: Y P
authors.initials: YP
authors.lastname: Vinetski