title: [Effect of Jianpi Qinghua decoctions on interleukin-6 and monocyte chemotactic protein-1 in rats with focal segmental glomurular sclerosis nephropathy: role of the inflammatory signaling pathways].
abstract: OBJECTIVE
  To study the effect of Jianpi Qinghua decoctions in ameliorating kidney fibrosis in rats with focal segmental glomurular sclerosis (FSGS) nephropathy in light of blocking the functions of the inflammatory cells.
  
  
  METHODS
  Rat models of FSGS nephropathy were established by left nephrectomy and intravenous injection of adriamycin and treated with Jianpi Qinghua decoction. The renal expressions of interleukin-6 (IL-6), monocyte chemotactic protein 1 (MCP-1), and intercellular adhesion molecular-1 (ICAM-1) were detected.
  
  
  RESULTS
  Treatment with Jianpi Qinghua decoction, Jianpi decoction, Qinghua decoction, and Niaoduqing particles all significantly lowered renal tubulointerstitial expressions of IL-6 in the model rats (P<0.01). Renal tubulointerstitial MCP-1 expressions were all lowered significantly in the rats by treatments with Jianpi Qinghua decoction, Jianpi decoction, QingHua decoctions, and Niaoduqing particles (P<0.01), and Jianpi Qinghua decoction produced a stronger effect than Niaoduqing particles (P<0.05). Jianpi Qinghua decoction, Jianpi decoction, Qinghua decoctions and and Niaoduqing particles all significantly decreased renal tubulointerstitial MCP-1 and ICAM-1 expressions (P<0.01), and Jianpi Qinghua and Qinghua decoctions both showed stronger effects than Niaoduqing particles (P<0.01). Jianpi Qinghua decoctions significantly suppressed the expressions of IL-6, MCP-1, and ICAM-1 and produced stronger effects than Niaoduqing particles on MCP-1 and ICAM-1 expressions.
  
  
  CONCLUSIONS
  Jianpi Qinghua decoctions can ameliorate inflammatory injury and lower the levels of inflammatory factors in rats with FSGS nephropathy, the mechanism of which is associated with inhibiting IL-6 signaling pathway.
authors.affiliation: Shuguang Hospital, Shanghai University of Traditional Chinese Medicine/Clinical Key Laboratory of Traditional Chinese Medicine, Shanghai 201203, China.E-mail: shandongzhaoshuai@sina.com.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Ma
authors.forename: Liqun
authors.initials: L
authors.lastname: He
mesh_terms: D000818:Animals; D018932:Chemokine CCL2 / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005923:Glomerulosclerosis, Focal Segmental / Q000378:metabolism* / Q000473:pathology; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism / Q000473:pathology*; D007684:Kidney Tubules / Q000378:metabolism; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012598:Sclerosis; D015398:Signal Transduction / Q000187:drug effects
chemical_list: C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D004365:Drugs, Chinese Herbal; D015850:Interleukin-6; D018799:Intercellular Adhesion Molecule-1