title: Variation in the calpain-10 gene is not associated with gestational diabetes mellitus.
abstract: BACKGROUND
  In several large studies an association between certain single-nucleotide polymorphisms (SNP) of the calpain-10 gene (CAPN10) with type 2 diabetes mellitus (T2D) has been identified. Since T2D and gestational diabetes mellitus (GDM) seem to be linked pathophysiologically, we examined the frequencies of CAPN10-polymorphisms in women with GDM.
  
  
  METHODS
  By using real-time PCR assisted melting curve analysis samples of 204 women with GDM and 297 controls were tested for variations of SNP-43, -44, -63 and Indel-19 of CAPN10.
  
  
  RESULTS
  Since the genotype frequencies found in SNP-44 among the controls did not meet the Hardy-Weinberg-Equilibrium, the further analysis was performed with SNP-43, -63 and Indel-19 only. Herein, the distribution of neither genotype nor allele nor haplogenotype-combination nor haplotype showed a significant difference between both groups.
  
  
  CONCLUSIONS
  Variations of SNP-43, -63 and Indel-19 of CAPN10 were not associated with an increased risk of developing GDM.
authors.affiliation: St. Vincenz-Hospital Limburg , Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Neuhaus
authors.forename: Christian
authors.initials: C
authors.lastname: Graf
authors.forename: Sebastian
authors.initials: S
authors.lastname: Stier
authors.forename: Michael
authors.initials: M
authors.lastname: Knapp
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Grunewald
authors.forename: Yon-Dschun
authors.initials: YD
authors.lastname: Ko
authors.forename: Marianne
authors.initials: M
authors.lastname: Sorger
mesh_terms: D000328:Adult; D002154:Calpain / Q000235:genetics*; D016022:Case-Control Studies; D016640:Diabetes, Gestational / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D054643:INDEL Mutation; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D012307:Risk Factors
chemical_list: D002154:Calpain; C415197:calpain 10