title: RNase III: Genetics and function; structure and mechanism.
abstract: RNase III is a global regulator of gene expression in Escherichia coli that is instrumental in the maturation of ribosomal and other structural RNAs. We examine here how RNase III itself is regulated in response to growth and other environmental changes encountered by the cell and how, by binding or processing double-stranded RNA (dsRNA) intermediates, RNase III controls the expression of genes. Recent insight into the mechanism of dsRNA binding and processing, gained from structural studies of RNase III, is reviewed. Structural studies also reveal new cleavage sites in the enzyme that can generate longer 3' overhangs.
authors.affiliation: Center for Cancer Research, National Cancer Institute, Frederick, Maryland 21702; email: courtd@mail.nih.gov , ganj@mail.nih.gov , liangyh@mail.nih.gov , shawg@mail.nih.gov , costantn@mail.nih.gov , tropeajo@mail.nih.gov , waughd@mail.nih.gov , jix@mail.nih.gov.
authors.forename: Donald L
authors.initials: DL
authors.lastname: Court
authors.forename: Jianhua
authors.initials: J
authors.lastname: Gan
authors.forename: Yu-He
authors.initials: YH
authors.lastname: Liang
authors.forename: Gary X
authors.initials: GX
authors.lastname: Shaw
authors.forename: Joseph E
authors.initials: JE
authors.lastname: Tropea
authors.forename: Nina
authors.initials: N
authors.lastname: Costantino
authors.forename: David S
authors.initials: DS
authors.lastname: Waugh
authors.forename: Xinhua
authors.initials: X
authors.lastname: Ji
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D020816:Amino Acid Motifs; D010582:Bacteriophage lambda / Q000235:genetics; D002384:Catalysis; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins / Q000378:metabolism; D005057:Eukaryotic Cells / Q000201:enzymology; D015964:Gene Expression Regulation, Bacterial; D009690:Nucleic Acid Conformation; D009876:Operon; D011387:Prokaryotic Cells / Q000201:enzymology; D011499:Protein Processing, Post-Translational; D012313:RNA / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012329:RNA, Bacterial / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism; D058727:RNA, Small Untranslated / Q000235:genetics; D043244:Ribonuclease III / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000502:physiology*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D014777:Virus Diseases / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D029968:Escherichia coli Proteins; D012329:RNA, Bacterial; D012330:RNA, Double-Stranded; D012335:RNA, Ribosomal; D058727:RNA, Small Untranslated; D012313:RNA; D043244:Ribonuclease III