title: Reduction of thrombogenicity of PVC-based sodium selective membrane electrodes using heparin-modified chitosan.
abstract: Heparin-modified chitosan (H-chitosan) membrane was utilized to enhance biocompatibility of sodium selective membrane electrode based on the highly thrombogenic polyvinyl chloride (PVC). Sodium ion sensing film was prepared using PVC, sodium ionophore-X, potassium tetrakis(chlorophenyl)-borate, and o-nitrophenyloctylether. The PVC-based sensing film was sandwiched to chitosan or H-chitosan to prevent platelet adhesion on the surface of PVC. Potentiometric response characteristics of PVC-chitosan and PVC-H-chitosan membrane electrodes were found to be comparable to that of a control PVC based sodium-selective electrode. This indicates that chitosan and H-chitosan layers do not alter the response behaviour of the PVC-based sensing film. Biocompatibility of H-chitosan was confirmed by in vitro platelet adhesion study. The platelet adhesion investigations indicated that H-chitosan film is less thrombogenic compared to PVC, which could result in enhancement of biocompatibility of sodium selective membrane electrodes based on PVC, while maintaining the overall electrochemical performance of the PVC-based sensing film.
authors.affiliation: Chemistry Department, Faculty of Science, Ain-Shams University, Cairo 11566, Egypt. Electronic address: ibadr1@yahoo.com.
authors.forename: Ibrahim H A
authors.initials: IH
authors.lastname: Badr
authors.forename: M
authors.initials: M
authors.lastname: Gouda
authors.forename: R
authors.initials: R
authors.lastname: Abdel-Sattar
authors.forename: Hossam E M
authors.initials: HE
authors.lastname: Sayour
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects; D002414:Cations, Monovalent; D048271:Chitosan / Q000031:analogs & derivatives / Q000737:chemistry* / Q000494:pharmacology; D004566:Electrodes; D004987:Ethers / Q000737:chemistry; D006490:Hemostatics / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry*; D008567:Membranes, Artificial; D010973:Platelet Adhesiveness / Q000187:drug effects; D011143:Polyvinyl Chloride / Q000737:chemistry*; D011199:Potentiometry / Q000295:instrumentation* / Q000379:methods; D034561:Sheep, Domestic; D012964:Sodium / Q000032:analysis*; D061210:Sodium Ionophores / Q000737:chemistry; D013775:Tetraphenylborate / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: C519952:2-nitrophenyl octyl ether; D002414:Cations, Monovalent; D004987:Ethers; D006490:Hemostatics; D008567:Membranes, Artificial; D061210:Sodium Ionophores; C030385:tetrakis(4-chlorophenyl)borate; D013775:Tetraphenylborate; D011143:Polyvinyl Chloride; D006493:Heparin; D048271:Chitosan; D012964:Sodium
keywords: Biocompatibility; Chitosan; Heparin-modified chitosan; Ion-selective electrodes; Membrane electrodes