title: Innovative fluorescent magnetic albumin microbead-assisted cell labeling and intracellular imaging of glioblastoma cells.
abstract: Superparamagnetic nanoparticle-based polymer microbeads utilized as carriers are attractive materials widely applied in the biomedical field. However, the deficiency of toxicity, biocompatibility, and biodegradability for polymer materials often limits the application of these microbeads. In the present study, magnetic albumin microbeads (MAMbs), i.e., human serum albumin-coated γ-Fe2O3 nanoparticles, are synthesized to label human U251 glioblastoma multiforme cells. The effects of MAMbs on the biological behavior of U251 glioblastoma cells, including their proliferation, cell viability, cytoskeletal structure, cell cycle, and apoptosis rate, are investigated. Moreover, fluorescein isothiocyanate (FITC)-MAMbs are fabricated by reaction with fluorescent dye FITC used for intracellular imaging of U251 glioblastoma cells. MAMbs possess undetectable cytotoxicity and excellent biocompatibility with U251 glioblastoma cells, as demonstrated by the biological behavior and morphology of U251 cells exposed to MAMbs. Furthermore, the constructed fluorescent MAMbs allow effective intracellular imaging, as illustrated by fluorescence microscopic analysis. The fabricated fluorescent MAMbs have promising perspectives in biomedical research, especially in cell-targeted labeling and intracellular fluorescence magnetic dual-mode imaging in cancer-targeted diagnosis and therapy.
authors.affiliation: College of Bioengineering, Henan University of Technology, Zhengzhou, Henan 450001, PR China. Electronic address: wangxq0108@gmail.com.
authors.forename: Xueqin
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Zhengzhou University, Zhengzhou, Henan 450001, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wei
authors.affiliation: College of Bioengineering, Henan University of Technology, Zhengzhou, Henan 450001, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Yan
authors.affiliation: College of Bioengineering, Henan University of Technology, Zhengzhou, Henan 450001, PR China.
authors.forename: Huiru
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Bioengineering, Henan University of Technology, Zhengzhou, Henan 450001, PR China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Tan
authors.affiliation: College of Bioengineering, Henan University of Technology, Zhengzhou, Henan 450001, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Bioengineering, Henan University of Technology, Zhengzhou, Henan 450001, PR China.
authors.forename: Guangzhou
authors.initials: G
authors.lastname: Zhou
authors.affiliation: College of Bioengineering, Henan University of Technology, Zhengzhou, Henan 450001, PR China.
authors.forename: Liuqing
authors.initials: L
authors.lastname: Cui
authors.affiliation: College of Bioengineering, Henan University of Technology, Zhengzhou, Henan 450001, PR China.
authors.forename: Cuixiang
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Bioengineering, Henan University of Technology, Zhengzhou, Henan 450001, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Bioengineering, Henan University of Technology, Zhengzhou, Henan 450001, PR China.
authors.forename: Yatao
authors.initials: Y
authors.lastname: Li
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D045744:Cell Line, Tumor; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives* / Q000032:analysis; D005456:Fluorescent Dyes / Q000032:analysis; D005909:Glioblastoma / Q000175:diagnosis / Q000473:pathology*; D006801:Humans; D060328:Magnetic Phenomena; D059346:Magnets / Q000032:analysis*; D008856:Microscopy, Fluorescence / Q000379:methods; D008863:Microspheres; D012709:Serum Albumin / Q000032:analysis; D013194:Staining and Labeling / Q000379:methods
chemical_list: C518288:FITC-albumin; D005456:Fluorescent Dyes; D012709:Serum Albumin; D016650:Fluorescein-5-isothiocyanate
keywords: Cell labeling; Fluorescein isothiocyanate (FITC); Intracellular imaging; Magnetic albumin microbeads (MAMbs); U251 glioblastoma cells