title: Mesophyll protoplasts of fenugreek (Trigonella foenumgraecum): Isolation, culture and shoot regeneration.
abstract: High yields of mesophyll protoplasts were obtained after treatment of leaves of Trigonella foenumgraecum, a forage legume, with purified cellulase. Under appropriate conditions of culture up to 70% plating efficiency could be obtained. The protoplast-derived colonies developed into rapidly growing green calli and produced leafy shoots on a medium containing 0.1 mg/l each of benzylaminopurine (BAP) and zeatin. Addition of glutamine and asparagine to the medium was found essential for rapid cell division, callus growth and differentiation.
authors.affiliation: Department of Biology, Yale University, P.O. Box 6666, 06511, New Haven, CT, USA.
authors.forename: N S
authors.initials: NS
authors.lastname: Shekhawat
authors.forename: A W
authors.initials: AW
authors.lastname: Galston