title: Lysine addressability and mammalian cell interactions of bacteriophage λ procapsids.
abstract: Chemically or genetically modified virus particles, termed viral nanoparticles (VNPs), are being explored in applications such as drug delivery, vaccine development, and materials science. Each virus platform has inherent properties and advantages based on its structure, molecular composition, and biomolecular interactions. Bacteriophage λ was studied for its lysine addressability, stability, cellular uptake, and the ability to modify its cellular uptake. λ procapsids could be labeled primarily at a single residue on the gpE capsid protein as determined by tandem mass spectrometry, providing a unique attachment site for further capsid modification. Bioconjugation of transferrin to the procapsids mediated specific interaction with transferrin receptor-expressing cells. These studies demonstrate the utility of bacteriophage λ procapsids and their potential use as targeted drug delivery vehicles.
authors.affiliation: Departments of Chemistry and Biology, Carthage College , Kenosha, Wisconsin, United States.
authors.forename: Kristopher J
authors.initials: KJ
authors.lastname: Koudelka
authors.forename: Shannon
authors.initials: S
authors.lastname: Ippoliti
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Medina
authors.forename: Leah P
authors.initials: LP
authors.lastname: Shriver
authors.forename: Sunia A
authors.initials: SA
authors.lastname: Trauger
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Catalano
authors.forename: Marianne
authors.initials: M
authors.lastname: Manchester
mesh_terms: D000595:Amino Acid Sequence; D010582:Bacteriophage lambda / Q000737:chemistry* / Q000378:metabolism; D002213:Capsid / Q000737:chemistry* / Q000378:metabolism; D036022:Capsid Proteins / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008239:Lysine / Q000737:chemistry*; D008969:Molecular Sequence Data; D011990:Receptors, Transferrin / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D014168:Transferrin / Q000737:chemistry / Q000378:metabolism; D053586:Virus Internalization
chemical_list: D036022:Capsid Proteins; D004337:Drug Carriers; D011990:Receptors, Transferrin; D014168:Transferrin; D008239:Lysine