title: Alkaloid accumulation in Ca-alginate entrapped cells of Catharanthus roseus: Using a limiting growth medium.
abstract: The limitation of growth of Catharanthus roseus cells was investigated with a view to their entrapment in a Ca-alginate matrix. An examination of the effects of lowered 2,4-D and phosphate concentrations on cell viability and indole alkaloid biosynthesis enabled a growth limiting and product formation stimulating medium to be designed. Entrapped cells showed a retention of both respiratory activity and biosynthetic capacity over an extended period of time compared with free cells. Evidence is presented which suggests that immobilization in Ca-alginate beads acts to stabilize cells, resulting in enhanced product accumulation.
authors.affiliation: Département de Génie Biochimique et Alimentaire, UA-CNRS-No 544, Institut National des Sciences Appliquées, Avenue de Rangueil, F-31077, Toulouse Cédex, France.
authors.forename: F
authors.initials: F
authors.lastname: Majerus
authors.forename: A
authors.initials: A
authors.lastname: Pareilleux