title: Antioxidant and anti-diabetic potential of Passiflora alata Curtis aqueous leaves extract in type 1 diabetes mellitus (NOD-mice).
abstract: Leaves of Passiflora alata Curtis were characterized for their antioxidant capacity. Antioxidant analyses of DPPH, FRAP, ABTS, ORAC and phenolic compounds were made in three different extracts: aqueous, methanol/acetone and ethanol. Aqueous extract was found to be the best solvent for recovery of phenolic compounds and antioxidant activity, when compared with methanol/acetone and ethanol. To study the anti-inflammatory properties of this extract in experimental type 1 diabetes, NOD mice were divided into two groups: the P. alata group, treated with aqueous extract of P. alata Curtis, and a non-treated control group, followed by diabetes expression analysis. The consumption of aqueous extract and water ad libitum lasted 28 weeks. The treated-group presented a decrease in diabetes incidence, a low quantity of infiltrative cells in pancreatic islets and increased glutathione in the kidney and liver (p<0.05), when compared with the diabetic and non-diabetic control-groups. In conclusion, our results suggest that the consumption of aqueous extract of P. alata may be considered a good source of natural antioxidants and compounds found in its composition can act as anti-inflammatory agents, helping in the control of diabetes.
authors.affiliation: Department of Internal Medicine, Faculty of Medical Sciences, University of Campinas - Rua Tessália Vieira de Camargo, 126, Cidade Universitária Zeferino Vaz, 13083-887 Campinas, SP, Brazil. Electronic address: talitacolomeu@yahoo.com.br.
authors.forename: T C
authors.initials: TC
authors.lastname: Colomeu
authors.affiliation: Department of Internal Medicine, Faculty of Medical Sciences, University of Campinas - Rua Tessália Vieira de Camargo, 126, Cidade Universitária Zeferino Vaz, 13083-887 Campinas, SP, Brazil. Electronic address: daniellafig@gmail.com.
authors.forename: D
authors.initials: D
authors.lastname: Figueiredo
authors.affiliation: Department of Food and Nutrition, School of Food Engineering, University of Campinas - Rua Monteiro Lobato, 80, Cidade Universitária Zeferino Vaz, 13083-862 Campinas, SP, Brazil. Electronic address: cinthiabetim@gmail.com.
authors.forename: C B B
authors.initials: CB
authors.lastname: Cazarin
authors.affiliation: Department of Internal Medicine, Faculty of Medical Sciences, University of Campinas - Rua Tessália Vieira de Camargo, 126, Cidade Universitária Zeferino Vaz, 13083-887 Campinas, SP, Brazil. Electronic address: nayaraschumacher@yahoo.com.br.
authors.forename: N S G
authors.initials: NS
authors.lastname: Schumacher
authors.affiliation: Department of Food and Nutrition, School of Food Engineering, University of Campinas - Rua Monteiro Lobato, 80, Cidade Universitária Zeferino Vaz, 13083-862 Campinas, SP, Brazil. Electronic address: mmarostica@gmail.com.
authors.forename: M R
authors.initials: MR
authors.lastname: Maróstica
authors.affiliation: Agronomic Institute of Campinas, Av. Theodureto de Almeida Camargo, 1500, Vila Nova, 13012-970 Campinas, SP, Brazil. Electronic address: lmmm@iac.sp.gov.br.
authors.forename: L M M
authors.initials: LM
authors.lastname: Meletti
authors.affiliation: Department of Internal Medicine, Faculty of Medical Sciences, University of Campinas - Rua Tessália Vieira de Camargo, 126, Cidade Universitária Zeferino Vaz, 13083-887 Campinas, SP, Brazil. Electronic address: zollner@unicamp.br.
authors.forename: R L
authors.initials: RL
authors.lastname: Zollner
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D002465:Cell Movement; D003922:Diabetes Mellitus, Type 1 / Q000188:drug therapy*; D004195:Disease Models, Animal; D006801:Humans; D007004:Hypoglycemic Agents / Q000008:administration & dosage*; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000473:pathology; D051379:Mice; D016688:Mice, Inbred NOD; D018384:Oxidative Stress; D029598:Passiflora / Q000737:chemistry / Q000276:immunology*; D019800:Phenol; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D018515:Plant Leaves; D014867:Water
chemical_list: D000975:Antioxidants; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D014867:Water; D019800:Phenol
keywords: Antioxidant capacity; Glutathione (GSH); Non-obese diabetic mice; Passiflora alata Curtis; Type 1 Diabetes mellitus