title: [Effects of Toll-like receptors on indoleamine 2, 3-dioxygenase mRNA levels in human trophoblast HTR-8/SVneo cells].
abstract: OBJECTIVE
  To study the expression of Toll-like receptors (TLRs) mRNA in human trophoblast HTR-8/SVneo cells and the changes in indoleamine 2,3-dioxygenase (IDO) mRNA expression in response to TLR ligand stimulation.
  
  
  METHODS
  The expressions of TLRs and IDO mRNA in human HTR-8/SVneo cells were tested by RT-PCR, and the changes in IDO mRNA levels after exposure to TLR3, TLR4, TLR7/8, and TLR9 ligands were quantitatively analyzed with real-time PCR.
  
  
  RESULTS
  IDO and TLR1-10 mRNAs were expressed in HTR-8/SVneo cells. As the cell culture time extended, IDO mRNA expression level tended to increase within 48 h. After stimulation with the TLR ligands, the expression of TLR-3 mRNA was down-regulated while the expression of TLR-4, 7, 8, and 9 mRNA up-regulated. Stimulation of the cells with poly(I:C) lowered the expression of IDO mRNA while IFN-γ increased its expression.
  
  
  CONCLUSIONS
  The expression of IDO mRNA is associated with the nutrition of the maternal-fetal interface. Stimulation with the TLR ligands affects the expression of IDO and TLR mRNA expressions in the cells, which verifies the functional activity of TLRs and suggests a role of IDO in TLR pathway-dependent antiviral immunity.
authors.affiliation: Laboratory of Reproductive Biology, Chongqing Medical University, Chongqing 400016, China.E-mail: xuwei19851225@126.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Guibo
authors.initials: G
authors.lastname: Yang
authors.forename: Jiazhong
authors.initials: J
authors.lastname: Duan
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Wenrong
authors.initials: W
authors.lastname: Yao
authors.forename: Xueqing
authors.initials: X
authors.lastname: Liu
authors.forename: Xuemei
authors.initials: X
authors.lastname: Chen
authors.forename: Yubin
authors.initials: Y
authors.lastname: Ding
authors.forename: Yingxiong
authors.initials: Y
authors.lastname: Wang
authors.forename: Junlin
authors.initials: J
authors.lastname: He
mesh_terms: D002460:Cell Line; D005260:Female; D006801:Humans; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000235:genetics / Q000378:metabolism*; D007371:Interferon-gamma / Q000494:pharmacology; D008024:Ligands; D011070:Poly I-C / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism*; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism*
chemical_list: D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D008024:Ligands; D012333:RNA, Messenger; D051193:Toll-Like Receptors; D007371:Interferon-gamma; D011070:Poly I-C