title: Interfacing of dextran coated ferrite nanomaterials with cellular system and delayed hypersensitivity on Guinea pigs.
abstract: The study focused on the interfacing of dextran coated ferrite nanomaterials (DFNM) with the cellular system and delayed hypersensitivity on Guinea pigs. In vitro study investigated the cytotoxic potential of DFNM on L929 cells, effect on antioxidant enzymes and Lipid peroxides (LPO) production on rat brain homogenates. DFNM was also repeatedly exposed topically to Guinea pigs for the evidence of skin sensitization and toxicity at the molecular level. Biochemical and hematological parameters were estimated. Liver and brain of Guinea pigs were homogenized and evaluated for the induction of LPO, glutathione (GSH), glutathione reductase (GR), glutathione peroxidase (GPx), superoxide dismutase (SOD) and 8-hydroxyl-2-deoxyguanosine (8-OHdG). The results of the study demonstrated that there was no significant alternation in the level of antioxidant defense enzymes, LPO, hematological, biochemical or oxidative stress related DNA damage. Hence, it can be concluded that the synthesized DFNM was non-skin irritant or non-toxic at the molecular level under the laboratory conditions.
authors.affiliation: Toxicology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala 695 012, India. Electronic address: mohanpv10@gmail.com.
authors.forename: P V
authors.initials: PV
authors.lastname: Mohanan
authors.affiliation: Toxicology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala 695 012, India.
authors.forename: C S
authors.initials: CS
authors.lastname: Geetha
authors.affiliation: Toxicology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala 695 012, India.
authors.forename: S
authors.initials: S
authors.lastname: Syama
authors.affiliation: Bioceramics Laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala 695 012, India.
authors.forename: H K
authors.initials: HK
authors.lastname: Varma
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D051381:Rats; D012867:Skin; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D003911:Dextrans; D005290:Ferric Compounds; C001215:ferrite
keywords: 8-OHdG; Antioxidant enzymes; DFNM; Hypersensitivity; LPO