title: Integrated sorting, concentration and real time PCR based detection system for sensitive detection of microorganisms.
abstract: The extremely low limit of detection (LOD) posed by global food and water safety standards necessitates the need to perform a rapid process of integrated detection with high specificity, sensitivity and repeatability. The work reported in this article shows a microchip platform which carries out an ensemble of protocols which are otherwise carried in a molecular biology laboratory to achieve the global safety standards. The various steps in the microchip include pre-concentration of specific microorganisms from samples and a highly specific real time molecular identification utilizing a q-PCR process. The microchip process utilizes a high sensitivity antibody based recognition and an electric field mediated capture enabling an overall low LOD. The whole process of counting, sorting and molecular identification is performed in less than 4 hours for highly dilute samples.
authors.affiliation: 1] Department of Mechanical Engineering, Indian Institute of Technology Kanpur, India [2].
authors.forename: Monalisha
authors.initials: M
authors.lastname: Nayak
authors.forename: Deepak
authors.initials: D
authors.lastname: Singh
authors.forename: Himanshu
authors.initials: H
authors.lastname: Singh
authors.forename: Rishi
authors.initials: R
authors.lastname: Kant
authors.forename: Ankur
authors.initials: A
authors.lastname: Gupta
authors.forename: Shashank Shekhar
authors.initials: SS
authors.lastname: Pandey
authors.forename: Swarnasri
authors.initials: S
authors.lastname: Mandal
authors.forename: Gurunath
authors.initials: G
authors.lastname: Ramanathan
authors.forename: Shantanu
authors.initials: S
authors.lastname: Bhattacharya
mesh_terms: D058491:Bacterial Load; D004783:Environmental Microbiology / Q000592:standards; D004926:Escherichia coli / Q000235:genetics; D056656:Lab-On-A-Chip Devices; D046208:Microchip Analytical Procedures / Q000379:methods* / Q000592:standards; D008839:Microelectrodes; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity