title: Human herpesvirus-6 and cytomegalovirus DNA in liver donor biopsies and their correlation with HLA matches and acute cellular rejection.
abstract: Herpesvirus reactivation is common after liver transplantation.
  
  
  OBJECTIVE
  Analyze the presence of cytomegalovirus (HCMV) and human herpesvirus-6 (HHV-6) DNA in liver donor biopsies, seeking to better understand issues involving human donor leukocyte antigens (HLA)-A, B and DR, as well as correlations with acute cellular rejection.
  
  
  METHODS
  Fifty-nine liver transplantation patients were investigated for the presence of HCMV and HHV-6 DNA in liver donor biopsies, using the Nested-PCR technique. The clinical donor information and HLA matches were obtained from the São Paulo State Transplant System. The recipients' records regarding acute cellular rejection were studied.
  
  
  RESULTS
  Seven (11.8%) biopsies were positive for HCMV DNA and 29 (49%) were positive for HHV-6 DNA. In 14 donors with HLA-DR 15 nine had HHV-6 DNA positive liver biopsy with a tendency for significant association (p=0.09), 22 recipients developed acute cellular rejection and 9/22 were positive for HLA-DR 15 (p=0.03; χ(2)=4.51), which was statistically significant in univariate analysis and showed a tendency after multivariate analysis (p=0.08).
  
  
  CONCLUSIONS
  HHV-6 DNA was prevalent in liver donors studied as well as HLA-DR 15. These findings suggest that patients with HLA-DR 15 in liver donor biopsies develop more rejection after liver transplantation.
authors.affiliation: Faculty of Medical Science, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil. Electronic address: carolguardia@yahoo.com.br.
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Guardia
authors.affiliation: Unit of Liver Transplantation, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil. Electronic address: stucchi.raquel@gmail.com.
authors.forename: Raquel Silveira Bello
authors.initials: RS
authors.lastname: Stucchi
authors.affiliation: Faculty of Medical Science, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil. Electronic address: arletemilan@ig.com.br.
authors.forename: Arlete
authors.initials: A
authors.lastname: Milan
authors.affiliation: Internal Medicine Department, Diagnosis of Molecular Infection Disease, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil. Electronic address: costa@fcm.unicamp.br.
authors.forename: Sandra Cecília Botelho
authors.initials: SC
authors.lastname: Costa
authors.affiliation: Unit of Liver Transplantation, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil. Electronic address: ilkaboin@yahoo.com.
authors.forename: Ilka de Fátima Santana Ferreira
authors.initials: Ide F
authors.lastname: Boin
mesh_terms: D000208:Acute Disease; D000328:Adult; D001706:Biopsy; D015331:Cohort Studies; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000821:virology*; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D006084:Graft Rejection / Q000175:diagnosis / Q000821:virology*; D006684:HLA-DR Antigens / Q000276:immunology*; D015654:Herpesvirus 6, Human / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000473:pathology / Q000821:virology*; D016031:Liver Transplantation / Q000009:adverse effects*; D008297:Male; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D019349:Roseolovirus Infections / Q000175:diagnosis / Q000821:virology*; D014775:Virus Activation
chemical_list: D004279:DNA, Viral; D006684:HLA-DR Antigens
keywords: Cytomegalovirus; Human herpesvirus 6; Liver donor biopsies; Liver transplantation