title: Investigation into the prevalence of a novel dendritic-like cell subset in vivo.
abstract: A novel dendritic-like cell subset termed L-DC was recently identified in murine spleen based on marker expression of a homogeneous cell population derived from long-term culture of neonatal spleen. The function of L-DC is distinct from other splenic dendritic and myeloid cell subsets because of their high endocytic capacity and their ability to cross-present antigen to CD8(+) T cells. This paper shows the subset to be unique to spleen and blood, with a similar, but possibly functionally distinct subset also present in bone marrow. The prevalence of the subset is low; ~6% of all dendritic and myeloid cells in the spleen and ~5% in blood. However, they are a distinct cell type on the basis of marker expression, and endocytic and T-cell stimulatory capacity. Attempts to identify an enriched population of these cells in mutant mouse strains with reported increases in myelopoiesis showed either a lack of L-DC or an altered phenotype reflective of the phenotype of the mouse strain.
authors.affiliation: Research School of Biology, The Australian National University, Acton, ACT, Australia.
authors.forename: Kristin Lisa
authors.initials: KL
authors.lastname: Griffiths
authors.forename: Jonathan Kah Huat
authors.initials: JK
authors.lastname: Tan
authors.forename: Helen Christine
authors.initials: HC
authors.lastname: O'Neill
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D004705:Endocytosis; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D018398:Homeodomain Proteins / Q000378:metabolism; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D022423:Myeloid Cells / Q000166:cytology / Q000378:metabolism; D010641:Phenotype; D013154:Spleen / Q000166:cytology / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D000949:Histocompatibility Antigens Class II; D018398:Homeodomain Proteins; C064658:RAG-1 protein
keywords: Dendritic cells; bone marrow; cross-presentation; spleen