title: Intron 4 VNTR (4a/b) polymorphism of the endothelial nitric oxide synthase gene is associated with breast cancer in Mexican women.
abstract: The endothelial nitric oxide synthase (eNOS) gene plays an important role in several biological functions. Polymorphisms of the eNOS gene have been associated with cancer. It has been suggested that the VNTR 4 a/b polymorphism may affect the expression of eNOS and contributes to tumor promotion in the mammary gland. We examined the role of the eNOS4 a/b polymorphism by comparing the genotypes of 281 healthy Mexican women with the genotypes of 429 Mexican women with breast cancer (BC). The observed genotype frequencies for control and BC patients were 0.6% and 0.7% for a/a (polymorphic); 87% and 77% for a/a (wild type); and 12% and 22% for a/b respectively. We found that the odds ratio (OR) was 1.9, with a 95% confidence interval (95%CI) of 1.29-2.95, P = 0.001 for genotypes a/a-a/b, b/c. The association was also evident when comparing the distribution of the a/a-a/b genotypes in patients with high levels of glutamate-oxaloacetate transaminase (SGOT) (OR, 1.93; 95% CI, 1.14-3.28; P = 0.015); undergoing menopause with high levels of SGOT (OR, 2.0; 95% CI, 1.1-3.84); and with high levels of glutamic-pyruvic transaminase (SGPT) (OR, 3.5; 95% CI, 1.56-8.22). The genotypes a/a-a/b are associated with BC susceptibility in the analyzed samples from the Mexican population.
authors.affiliation: Molecular Genetics Laboratory, Molecular Medicine Division, Western Biomedical Research Center (CIBO), Western National Medical Center (CMNO), Mexican Social Security Institute (IMSS), Guadalajara, Jalisco, Mexico. ; PhD Program in Human Genetics, Health Sciences University Center (CUCS), University of Guadalajara (U de G), Guadalajara, Jalisco, Mexico.
authors.forename: Ramiro
authors.initials: R
authors.lastname: Ramírez-Patiño
authors.forename: Luis Eduardo
authors.initials: LE
authors.lastname: Figuera
authors.forename: Ana María
authors.initials: AM
authors.lastname: Puebla-Pérez
authors.forename: Jorge Iván
authors.initials: JI
authors.lastname: Delgado-Saucedo
authors.forename: María Magdalena
authors.initials: MM
authors.lastname: Legazpí-Macias
authors.forename: Rocio Patricia
authors.initials: RP
authors.lastname: Mariaud-Schmidt
authors.forename: Adriana
authors.initials: A
authors.lastname: Ramos-Silva
authors.forename: Itzae Adonai
authors.initials: IA
authors.lastname: Gutiérrez-Hurtado
authors.forename: Liliana
authors.initials: L
authors.lastname: Gómez Flores-Ramos
authors.forename: Guillermo Moisés
authors.initials: GM
authors.lastname: Zúñiga-González
authors.forename: Martha Patricia
authors.initials: MP
authors.lastname: Gallegos-Arreola
mesh_terms: D000328:Adult; D000410:Alanine Transaminase / Q000097:blood*; D001219:Aspartate Aminotransferases / Q000097:blood*; D001943:Breast Neoplasms / Q000097:blood* / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008800:Mexico; D008875:Middle Aged; D009569:Nitric Oxide / Q000096:biosynthesis / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
chemical_list: D009569:Nitric Oxide; D052250:Nitric Oxide Synthase Type III; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: Breast Cancer; Mexican Population; VNTR; eNOS