title: Effect of Cytochalasin B, Lantrunculin B, Colchicine, Cycloheximid, Dimethyl Sulfoxide and Ion Channel Inhibitors on Biospeckle Activity in Apple Tissue.
abstract: The biospeckle phenomenon is used for non-destructive monitoring the quality of fresh fruits and vegetables, but in the case of plant tissues there is a lack of experimentally confirmed information about the biological origin of the biospeckle activity (BA). As a main sources of BA, processes associated with the movement inside the cell, such as cytoplasmic streaming, organelle movement and intra- and extracellular transport mechanisms, are considered. The aim of this study is to investigate the effect of metabolism inhibitors, connected with intracellular movement such as cytochalasin B, lantrunculin B, colchicine, cycloheximid, dimethyl sulfoxide (DMSO) and mixture of ion channel inhibitors, injected into apples, on BA. Two methods of BA analysis based on cross-correlation coefficient and Laser Speckle Contrast Analysis (LASCA) were used. DMSO, lantrunculin B and mixture of ion channel inhibitors have a significant effect on BA, and approximately 74 % of BA of apple tissue is potentially caused by biological processes. Results indicate that the functioning of actin microfilaments and ion channels significantly affect BA.
authors.affiliation: Department of Microstructure and Mechanics of Biomaterials, Institute of Agrophysics, Polish Academy of Sciences, Doswiadczalna 4, 20-290 Lublin 27, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Kurenda
authors.affiliation: Department of Microstructure and Mechanics of Biomaterials, Institute of Agrophysics, Polish Academy of Sciences, Doswiadczalna 4, 20-290 Lublin 27, Poland.
authors.forename: Piotr M
authors.initials: PM
authors.lastname: Pieczywek
authors.affiliation: Department of Microstructure and Mechanics of Biomaterials, Institute of Agrophysics, Polish Academy of Sciences, Doswiadczalna 4, 20-290 Lublin 27, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Adamiak
authors.affiliation: Department of Microstructure and Mechanics of Biomaterials, Institute of Agrophysics, Polish Academy of Sciences, Doswiadczalna 4, 20-290 Lublin 27, Poland.
authors.forename: Artur
authors.initials: A
authors.lastname: Zdunek
keywords: Apple tissue; Biological processes; Biospeckle; Image analysis; Intracellular movements; Metabolism inhibitors