title: RORγt modulates macrophage recruitment during a hydrocarbon oil-induced inflammation.
abstract: Hydrocarbon oils are often utilized as adjuvants in vaccines. In response to naturally occurring hydrocarbon oils, inflammation is initiated and persists with the continuous recruitment of immune cells such as macrophages and neutrophils. However, the mechanism underlying the chronic inflammation in response to hydrocarbon oils is not fully defined. In this study, we revealed an essential role of retinoid-related orphan receptor gamma t (RORγt) in sustaining the recruitment of macrophages following pristane treatment. RORγt absence resulted in the incompetent formation of mesenteric oil granulomas which may associate to a reduction in the migration of macrophages into the mesentery during pristane-induced inflammation. This is at least partially dependent on the expression of the monocyte chemoattractant protein-1 (MCP-1) in the mesentery and the decrease in the macrophage reservoir in the spleen. However, the absence of RORγt had no impact on the recruitment of neutrophils to the mesentery after pristane treatment. Our data uncovered an important role of RORγt in the recruitment of macrophages during hydrocarbon oil-induced chronic inflammation.
authors.affiliation: Tianjin Haihe Hospital, Tianjin Institute of Respiratory Diseases, Tianjin, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wu
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
authors.forename: Ruo
authors.initials: R
authors.lastname: Chi
authors.forename: Long
authors.initials: L
authors.lastname: Xu
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Feng
authors.forename: Huaiyong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism; D005434:Flow Cytometry; D006099:Granuloma / Q000139:chemically induced / Q000378:metabolism; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects*; D008643:Mesentery / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D051379:Mice; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3 / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D013729:Terpenes / Q000494:pharmacology
chemical_list: C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; D013729:Terpenes; C009042:pristane