title: Intracellular distribution of phosphate in cultured Humulus lupulus cells growing at elevated exogenous phosphate concentrations.
abstract: The distribution of inorganic phosphate (Pi) between the cytoplasm and the vacuole of Humulus lupulus L. cells grown in suspension culture at different exogenous Pi levels was examined by 31-P nuclear magnetic resonance. In growing cells excess Pi accumulated in the vacuoles and the inhibitory effect of high exogenous Pi was not associated with a change in the cytoplasmic Pi level or with a change in the cytoplasmic pH.
authors.affiliation: A.F.R.C. Food Research Institute, Colney Lane, NR4 7UA, Norwich, UK.
authors.forename: R J
authors.initials: RJ
authors.lastname: Robins
authors.forename: R G
authors.initials: RG
authors.lastname: Ratcliffe