title: Arabidopsis EDM2 promotes IBM1 distal polyadenylation and regulates genome DNA methylation patterns.
abstract: DNA methylation is important for the silencing of transposons and other repetitive elements in many higher eukaryotes. However, plant and mammalian genomes have evolved to contain repetitive elements near or inside their genes. How these genes are kept from being silenced by DNA methylation is not well understood. A forward genetics screen led to the identification of the putative chromatin regulator Enhanced Downy Mildew 2 (EDM2) as a cellular antisilencing factor and regulator of genome DNA methylation patterns. EDM2 contains a composite Plant Homeo Domain that recognizes both active and repressive histone methylation marks at the intronic repeat elements in genes such as the Histone 3 lysine 9 demethylase gene Increase in BONSAI Methylation 1 (IBM1) and is necessary for maintaining the expression of these genes by promoting mRNA distal polyadenylation. Because of its role in maintaining IBM1 expression, EDM2 is required for preventing CHG methylation in the bodies of thousands of genes. Our results thus increase the understanding of antisilencing, genome methylation patterns, and regulation of alternative RNA processing by intronic heterochromatin.
authors.affiliation: Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47906.
authors.forename: Mingguang
authors.initials: M
authors.lastname: Lei
authors.forename: Honggui
authors.initials: H
authors.lastname: La
authors.forename: Kun
authors.initials: K
authors.lastname: Lu
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Wang
authors.forename: Daisuke
authors.initials: D
authors.lastname: Miki
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Ren
authors.forename: Cheng-Guo
authors.initials: CG
authors.lastname: Duan
authors.forename: Xingang
authors.initials: X
authors.lastname: Wang
authors.forename: Kai
authors.initials: K
authors.lastname: Tang
authors.forename: Liang
authors.initials: L
authors.lastname: Zeng
authors.forename: Lan
authors.initials: L
authors.lastname: Yang
authors.forename: Heng
authors.initials: H
authors.lastname: Zhang
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Nie
authors.forename: Pan
authors.initials: P
authors.lastname: Liu
authors.forename: Jianping
authors.initials: J
authors.lastname: Zhou
authors.forename: Renyi
authors.initials: R
authors.lastname: Liu
authors.forename: Yingli
authors.initials: Y
authors.lastname: Zhong
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.forename: Jian-Kang
authors.initials: JK
authors.lastname: Zhu
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001483:Base Sequence; D019175:DNA Methylation; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics; D005020:Ethyl Methanesulfonate / Q000737:chemistry; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D018745:Genome, Plant; D006570:Heterochromatin / Q000378:metabolism; D006657:Histones / Q000737:chemistry; D056484:Jumonji Domain-Containing Histone Demethylases / Q000378:metabolism*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D026723:Polyadenylation; D012333:RNA, Messenger / Q000378:metabolism; D013447:Sulfites / Q000737:chemistry; D014157:Transcription Factors / Q000378:metabolism*; D019076:Transgenes
chemical_list: D029681:Arabidopsis Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D018744:DNA, Plant; C523205:EDM2 protein, Arabidopsis; D006570:Heterochromatin; D006657:Histones; D010455:Peptides; D012333:RNA, Messenger; D013447:Sulfites; D014157:Transcription Factors; D005020:Ethyl Methanesulfonate; C526313:IBM1 protein, Arabidopsis; D056484:Jumonji Domain-Containing Histone Demethylases
keywords: DNA methylome; alternative polyadenylation; gene body methylation; post-transcription regulation; transcriptional gene silencing