title: BCL-2 and Bax Expression in Skin Flaps Treated with Finasteride or Azelaic Acid.
abstract: Despite all modern surgical techniques, skin flap that is considered as the main method in most reconstructive surgeries puts the skin tissue at danger of necrosis and apoptosis derived from ischemia. Therefore, finding a treatment for decreasing the apoptosis derived from flap ischemia will be useful in clinic. In present study, we evaluated the effect of azelaic acid 20% and finasteride on expression of BCL-2 and bax proteins after the skin flap surgery. For this purpose, 21 rats were entered in three groups including control, azelaic acid 20% and finasteride, all experienced skin flap surgery and then flap tissue was assessed for determining the expression of proteins in 5 slices prepared from each rat that were graded between - to +++ scales. Both azelaic acid and finasteride increased the expression of BCL-2 protein (p < 0.05) and decrease the expression of bax protein (p < 0.05). These results suggested an antiapoptotic role for finasteride and azelaic acid in preserving the flap after the ischemia reperfusion insult.
authors.affiliation: Department of pharmacognosy, School of Pharmacy, Shahid Beheshti University, M.C., Tehran, Iran. ; School of Pharmacy and Phytochemistry Research Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Seyyed Abdulmajid
authors.initials: SA
authors.lastname: Ayatollahi
authors.forename: Marjan
authors.initials: M
authors.lastname: Ajami
authors.forename: Hamed
authors.initials: H
authors.lastname: Reyhanfard
authors.forename: Yasin
authors.initials: Y
authors.lastname: Asadi
authors.forename: Mansour
authors.initials: M
authors.lastname: Nassiri-Kashani
authors.forename: Mehdi
authors.initials: M
authors.lastname: Rashighi Firoozabadi
authors.forename: Sayed Hossein
authors.initials: SH
authors.lastname: Davoodi
authors.forename: Esmaeil
authors.initials: E
authors.lastname: Habibi
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Pazoki-Toroudi
keywords: Apoptosis; Azelaic acid; Finasteride; Skin flaps