title: Plant regeneration from mesocotyl callus of Hordeum vulgare L.
abstract: Callus tissue was induced on barley mesocotyl explants of germinated seven-day-old seedlings on MS medium supplemented with 2,4-D or 2,4,5-T in high concentrations. Two morphologically different tissue cultures were maintained in vitro for a long time: a callus tissue without organogenesis and a culture with high rhizogenic capacity. Shoots and plantlets were generated when the auxin-media induced callus was transferred to medium supplemented with 3 μM TIBA. In 62% of cultures, during the first five subcultures, four to twentyeight plants per single mesocotyl were obtained. Some cultures produced shoots even in the 9th subculture, being in culture for nearly 14 months. The largest number of plants obtained per one mesocotyl was forty. Plantlets rooted well on MS with 5.7 μM IAA and survived transplantation to soil in high percentages.
authors.affiliation: Department of Botany, Faculty of Science, University of Zagreb, P.O. Box 933, 41001, Zagreb, Yugoslavia.
authors.forename: S
authors.initials: S
authors.lastname: Jelaska
authors.forename: Z
authors.initials: Z
authors.lastname: Rengel
authors.forename: V
authors.initials: V
authors.lastname: Cesar