title: Reuse of enzymes for isolation of protoplasts.
abstract: A method has been developed for the reuse of cell wall digesting enzymes to isolate protoplasts from actively-growing suspension cultures of plant cells. Protoplasts could be satisfactorily prepared as many as three times using the same enzyme mixture without any loss in yield or viability of the isolated protoplasts. The yields of nuclei isolated from protoplasts prepared with used enzyme solution were comparable to those obtained with fresh enzymes.
authors.affiliation: Department of Biology, University of Saskatchewan, S7N OWO, Saskatoon, Saskatchewan, Canada.
authors.forename: P K
authors.initials: PK
authors.lastname: Saxena
authors.forename: J
authors.initials: J
authors.lastname: King