title: In vitro and in vivo three-dimensional velocity vector measurement by three-beam spectral-domain Doppler optical coherence tomography.
abstract: We developed a three-beam Doppler optical coherence tomography (OCT) system that enables measurement of the velocity vector of moving particles in three-dimensions (3-D). The spatial orientation as well as the magnitude of motion can be determined without prior knowledge of the geometry of motion. The system combines three spectral-domain OCT interferometers whose sample beams are focused at the sample by a common focusing lens at three different angles. This provides three spatially independent velocity components simultaneously from which the velocity vector can be reconstructed. We demonstrate the system in a simple test object (rotating disc), a flow phantom, and for blood flow measurements in the retina of a healthy human subject. Measurements of blood flow at a venous bifurcation achieve a good agreement between in- and outflow and demonstrate the reliability of the method.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Trasischker
authors.forename: René M
authors.initials: RM
authors.lastname: Werkmeister
authors.forename: Stefan
authors.initials: S
authors.lastname: Zotter
authors.forename: Bernhard
authors.initials: B
authors.lastname: Baumann
authors.forename: Teresa
authors.initials: T
authors.lastname: Torzicky
authors.forename: Michael
authors.initials: M
authors.lastname: Pircher
authors.forename: Christoph K
authors.initials: CK
authors.lastname: Hitzenberger
mesh_terms: D001783:Blood Flow Velocity / Q000502:physiology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007368:Interferometry / Q000295:instrumentation / Q000379:methods; D017078:Laser-Doppler Flowmetry / Q000295:instrumentation / Q000379:methods*; D019047:Phantoms, Imaging; D012171:Retinal Vessels / Q000502:physiology*; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods*