title: Formation of multilayered vesicles from water/organic-solvent (w/o) emulsions: theory and practice.
abstract: Multilayered liposome (MLV) formation from water/organic-solvent (W/O) emulsions was studied. A fundamental liposome population parameter, the E(ratio), was defined and used to estimate the bilayer number and water spacing in MLV liposomes. MLVs prepared from W/O emulsions have optimum drug entrapment at an emulsion-lipid/emulsion-water ratio of ∼0.33. Drug entrapment is typically 50 to 65% under these optimal conditions.
authors.affiliation: Eli Lilly Research Laboratories, Indianapolis, Indiana, 46285.
authors.forename: C
authors.initials: C
authors.lastname: Pidgeon
authors.forename: A H
authors.initials: AH
authors.lastname: Hunt
authors.forename: K
authors.initials: K
authors.lastname: Dittrich