title: Conservation and developmental expression of ubiquitin isopeptidases in Schistosoma mansoni.
abstract: Several genes related to the ubiquitin (Ub)-proteasome pathway, including those coding for proteasome subunits and conjugation enzymes, are differentially expressed during the Schistosoma mansoni life cycle. Although deubiquitinating enzymes have been reported to be negative regulators of protein ubiquitination and shown to play an important role in Ub-dependent processes, little is known about their role in S. mansoni . In this study, we analysed the Ub carboxyl-terminal hydrolase (UCHs) proteins found in the database of the parasite's genome. An in silico ana- lysis (GeneDB and MEROPS) identified three different UCH family members in the genome, Sm UCH-L3, Sm UCH-L5 and Sm BAP-1 and a phylogenetic analysis confirmed the evolutionary conservation of the proteins. We performed quantitative reverse transcription-polymerase chain reaction and observed a differential expression profile for all of the investigated transcripts between the cercariae and adult worm stages. These results were corroborated by low rates of Z-Arg-Leu-Arg-Gly-Gly-AMC hydrolysis in a crude extract obtained from cercariae in parallel with high Ub conjugate levels in the same extracts. We suggest that the accumulation of ubiquitinated proteins in the cercaria and early schistosomulum stages is related to a decrease in 26S proteasome activity. Taken together, our data suggest that UCH family members contribute to regulating the activity of the Ub-proteasome system during the life cycle of this parasite.
authors.affiliation: Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro PretoMG, Brasil.
authors.forename: Roberta Verciano
authors.initials: RV
authors.lastname: Pereira
authors.affiliation: Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro PretoMG, Brasil.
authors.forename: Helaine Graziele Santos
authors.initials: HG
authors.lastname: Vieira
authors.affiliation: Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro PretoMG, Brasil.
authors.forename: Victor Fernandes de
authors.initials: VF
authors.lastname: Oliveira
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Patos de MinasMG, Brasil.
authors.forename: Matheus de Souza
authors.initials: Mde S
authors.lastname: Gomes
authors.affiliation: Centro de Pesquisas René Rachou, Fiocruz, Belo HorizonteMG, Brasil.
authors.forename: Liana Konovaloff Jannotti
authors.initials: LK
authors.lastname: Passos
authors.affiliation: Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro PretoMG, Brasil.
authors.forename: William de Castro
authors.initials: Wde C
authors.lastname: Borges
authors.affiliation: Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro PretoMG, Brasil.
authors.forename: Renata
authors.initials: R
authors.lastname: Guerra-Sá
mesh_terms: D000818:Animals; D058487:Cercaria / Q000201:enzymology / Q000235:genetics; D017124:Conserved Sequence / Q000235:genetics; D010450:Endopeptidases / Q000235:genetics*; D019143:Evolution, Molecular; D015870:Gene Expression; D016678:Genome / Q000235:genetics; D049751:Genome, Helminth / Q000235:genetics; D008018:Life Cycle Stages / Q000235:genetics; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012550:Schistosoma mansoni / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D016415:Sequence Alignment; D059467:Transcriptome / Q000502:physiology; D057900:Transcytosis / Q000502:physiology; D043222:Ubiquitin Thiolesterase / Q000145:classification / Q000235:genetics*; D064570:Ubiquitin-Specific Proteases / Q000235:genetics; D054875:Ubiquitination / Q000502:physiology
chemical_list: D010450:Endopeptidases; D043222:Ubiquitin Thiolesterase; D064570:Ubiquitin-Specific Proteases; C091939:ubiquitin isopeptidase