title: Effects of fibronectin and type IV collagen on osteosarcoma cell apoptosis.
abstract: The aims of this study are the investigation of the effects of fibronectin and type IV collagen extracellular matrix proteins and the role of caspase-3 and -9 on cis-platin induced U2-OS apoptosis were studied. First the cytotoxic effects of cis-platin on cell system were investigated by colorimetric method and than morphological and ELISA analysis were used for determination of cell apoptosis when induced with cis-platin. In addition, after adhering the cells to fibronection or type IV collagen proteins, the apoptotic rate and the effects of caspase-3 and -9 were also investigated by ELISA in presence of specific inhibitors. U2-OS cells showed 20% cytotoxicity after treatment with 2.4 microM of cis-platin for 48 h. Morphological and the numerical data showed that cis-platin was able to induced apoptosis on cells as a dose-dependent manner. Caspase-3 and -9 inhibitors inhibited cis-platin-induced apoptosis in U2-OS cells, respectively. The binding of cells to 10 microg/mL of fibronectin but not type IV collagen enhanced the apoptosis about 2.5 fold that effects inhibited with caspase-3 inhibitor. The caspase-3 and -9 are involved in the apoptotic signals induced by cis-platin in U2-OS. The binding to fibronectin, but not type IV collagen enhanced the apoptotic response of U2-OS and fibronectin-dependent apoptosis was activated by caspase-3. These finding might be useful for patients to fight against osteosarcoma.
authors.affiliation: Department of Biochemistry, Faculty of Farmacy, Anadolu University, Eskişehir, Turkey. zseller@anadolu.edu.tr
authors.forename: Zerrin
authors.initials: Z
authors.lastname: Incesu
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Hatipoğlu
authors.forename: Hülya
authors.initials: H
authors.lastname: Sivas
authors.forename: Emel
authors.initials: E
authors.lastname: Ergene
authors.forename: Gülşen Akalin
authors.initials: GA
authors.lastname: Ciftci
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D001859:Bone Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology; D024141:Collagen Type IV / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D005353:Fibronectins / Q000494:pharmacology*; D006801:Humans; D012516:Osteosarcoma / Q000473:pathology*
chemical_list: D000970:Antineoplastic Agents; D024141:Collagen Type IV; D005353:Fibronectins; D002945:Cisplatin