title: Antibacterial activity of the essential oil of Heracleum sibiricum.
abstract: The composition and antimicrobial activity of the essential oil of Heracleum sibiricum L. (Apiaceae) was studied. The aerial part of plant was hydro-distilled and chemical composition of the essential oil was analyzed by GC and GC-MS. Forty-six compounds, corresponding to 95.12% of the total oil, were identified. Esters represented the major chemical class (69.55%) while the main constituents were octyl butanoate (36.82%), hexyl butanoate (16.08%), 1-octanol (13.62%) and octyl hexanoate (8.10%). Antibacterial activity of the essential oil and reference antibiotics against nine bacterial strains was tested by the broth microdilution method. The results of the bioassays showed that essential oil had slight antimicrobial activities against all tested microorganisms (MIC and MBC values were in the range of 2431.2 to 9724.8 microg/mL). Reference antibiotics were active in concentrations between 0.5 and 16.0 microg/mL. The results confirm that Gram-positive bacteria were more susceptible to the essential oil of H. sibiricum, in comparison with Gram-negative bacteria.
authors.affiliation: Department of Pharmacy, Faculty of Medicine, University of Nis, 18000 Nis, Serbia. dragoljubm@gmail.com
authors.forename: Dragoljub L
authors.initials: DL
authors.lastname: Miladinović
authors.forename: Budimir S
authors.initials: BS
authors.lastname: Ilić
authors.forename: Tatjana M
authors.initials: TM
authors.lastname: Mihajilov-Krstev
authors.forename: Dejan M
authors.initials: DM
authors.lastname: Nikolić
authors.forename: Olga G
authors.initials: OG
authors.lastname: Cvetković
authors.forename: Marija S
authors.initials: MS
authors.lastname: Marković
authors.forename: Ljiljana C
authors.initials: LC
authors.lastname: Miladinović
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D030002:Heracleum / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D009822:Oils, Volatile