title: Human leucocyte antigen-G polymorphisms are associated with cervical squamous cell carcinoma risk in Taiwanese women.
abstract: BACKGROUND
  The mere presence of human papillomavirus (HPV) is not enough for cervical cancer development and immunogenetic background may play an important role. Human leucocyte antigen (HLA)-G acts as a negative regulator of immune responses and its expression in tumour cells may enable them to avoid immune attack. We aim to study if polymorphisms in the HLA-G gene are associated with cervical cancer risk in Taiwanese women.
  
  
  METHODS
  +1537 A/C, 14-bp deletion/insertion (Del/Ins), and +3142 G/C polymorphisms were genotyped in a hospital-based study of 317 women with cervical squamous cell carcinoma (CSCC) and 400 healthy control women frequency matched by age. The presence and genotypes of HPV in CSCC were determined.
  
  
  RESULTS
  We found the +3142 C/C genotype and C allele were associated with increased risk for CSCC (adjusted odds ratio [OR]=1.78, P=0.004; adjusted OR=1.31, P=0.014, respectively). In subgroup analysis based on HPV type 16 positivity, significant associations with higher adjusted ORs were found in +3142 C/C genotype and C allele (adjusted OR=2.19, P=0.001; adjusted OR=1.48, P=0.003, respectively) and +1537 C/C genotype and C allele frequencies increased significantly (adjusted OR=2.88, P=0.004; adjusted OR=1.69, P=0.0005, respectively). Furthermore, the C-Del-C haplotype conferred increased risk of both CSCC and HPV-16 positive CSCC women (adjusted OR=1.41, P=0.009; adjusted OR=1.94, P=0.0001, respectively).
  
  
  CONCLUSIONS
  These findings suggest that HLA-G gene is involved in the susceptibility to CSCC.
authors.affiliation: Department of Gynecology and Obstetrics, Mackay Memorial Hospital, Taipei City, Taiwan; Department of Gynecology and Obstetrics, Taipei Medical University, Taipei City, Taiwan.
authors.forename: Yuh-Cheng
authors.initials: YC
authors.lastname: Yang
authors.affiliation: Department of Medical Research, Mackay Memorial Hospital, New Taipei City, Taiwan.
authors.forename: Tzu-Yang
authors.initials: TY
authors.lastname: Chang
authors.affiliation: Department of Gynecology and Obstetrics, Mackay Memorial Hospital, Taipei City, Taiwan.
authors.forename: Tze-Chien
authors.initials: TC
authors.lastname: Chen
authors.affiliation: Department of Medical Research, Mackay Memorial Hospital, New Taipei City, Taiwan.
authors.forename: Wen-Shan
authors.initials: WS
authors.lastname: Lin
authors.affiliation: Department of Medical Research, Mackay Memorial Hospital, New Taipei City, Taiwan.
authors.forename: Shih-Chuan
authors.initials: SC
authors.lastname: Chang
authors.affiliation: Department of Pediatrics, Mackay Memorial Hospital, Taipei City, Taiwan; Department of Medical Research, Mackay Memorial Hospital, New Taipei City, Taiwan; Department of Pediatrics, Taipei Medical University, Taipei City, Taiwan; Institute of Biomedicine Sciences and Department of Medicine, Mackay Medical College, New Taipei City, Taiwan. Electronic address: kylechang@ms1.mmh.org.tw.
authors.forename: Yann-Jinn
authors.initials: YJ
authors.lastname: Lee
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000150:complications / Q000208:ethnology / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000208:ethnology / Q000235:genetics*; D005838:Genotype; D059951:HLA-G Antigens / Q000235:genetics*; D006801:Humans; D054643:INDEL Mutation; D008875:Middle Aged; D016017:Odds Ratio; D030361:Papillomavirus Infections / Q000150:complications / Q000208:ethnology; D011110:Polymorphism, Genetic; D012307:Risk Factors; D013624:Taiwan; D002583:Uterine Cervical Neoplasms / Q000150:complications / Q000208:ethnology / Q000235:genetics*
chemical_list: D059951:HLA-G Antigens
keywords: Cervical cancer; HLA-G; HPV; Immunity; Polymorphism