title: Precise structure control of three-state nanomechanical DNA origami devices.
abstract: Precise structure switching between all of the three forms of three-state nanomechanical DNA origami devices has been accomplished. A nanomechanical DNA origami device called DNA origami pliers, which consists of two levers of 170-nm long, 20-nm wide, and 2-nm thick connected at a Holliday-junction fulcrum, takes three conformations: closed parallel, closed antiparallel, and open cross forms. They were previously applied to construct detection systems for biomolecules in single-molecular resolution by observing the structure switching between cross form and one of the other two forms under atomic force microscope (AFM). We redesigned DNA origami pliers in this study to let them freely switch between all of the three states including parallel-antiparallel direct switching without taking cross form. By the addition of appropriate switcher strands to the solution, hybridization and dehybridization of particular binder strands that fix the levers into predetermined state were selectively triggered as programmed in their sequence. Circuit structure switching through all of the three states in both of the two opposite direction was even successful with the new design.
authors.affiliation: Department of Chemistry and Materials Engineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, Japan; PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawguchi, Saitama 332-0012, Japan. Electronic address: kuzuya@kansai-u.ac.jp.
authors.forename: Akinori
authors.initials: A
authors.lastname: Kuzuya
authors.affiliation: Department of Chemistry and Materials Engineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Watanabe
authors.affiliation: Department of Chemistry and Materials Engineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, Japan.
authors.forename: Mirai
authors.initials: M
authors.lastname: Hashizume
authors.affiliation: Department of Chemistry and Materials Engineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Kaino
authors.affiliation: Department of Chemistry and Materials Engineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Minamida
authors.affiliation: Department of Chemistry and Materials Engineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Kameda
authors.affiliation: Department of Chemistry and Materials Engineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, Japan. Electronic address: yohya@kansai-u.ac.jp.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ohya
mesh_terms: D017104:Bacteriophage M13 / Q000235:genetics; D045566:DNA, Cruciform / Q000737:chemistry / Q000648:ultrastructure; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000648:ultrastructure; D004279:DNA, Viral / Q000737:chemistry* / Q000648:ultrastructure; D055595:Mechanical Phenomena; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D045566:DNA, Cruciform; D004277:DNA, Single-Stranded; D004279:DNA, Viral
keywords: AFM; DNA nanotechnology; DNA origami; Molecular devices; Molecular robots; Nanomachines