title: Antibody reactivity to the major fish allergen parvalbumin is determined by isoforms and impact of thermal processing.
abstract: The EF-hand calcium binding protein, parvalbumin, is a major fish allergen. Detection of this allergen is often difficult due to its structural diversity among various fish species. The aim of this study was to evaluate the cross-reactivity of parvalbumin in a comprehensive range of bony and cartilaginous fish, from the Asia-Pacific region, and conduct a molecular analysis of this highly allergenic protein. Using the monoclonal anti-parvalbumin antibody PARV-19, we demonstrated the presence of monomeric and oligomeric parvalbumin in all fish analysed, except for gummy shark a cartilaginous fish. Heat processing of this allergen greatly affected its antibody reactivity. While heating caused a reduction in antibody reactivity to multimeric forms of parvalbumins for most bony fish, a complete loss of reactivity was observed for cartilaginous fish. Molecular analysis demonstrated that parvalbumin cross-reactivity, among fish species, is due to the molecular phylogenetic association of this major fish allergen.
authors.affiliation: School of Pharmacy and Molecular Science, Centre for Biodiscovery and Molecular Development of Therapeutics, James Cook University, Townsville, Queensland, Australia. Electronic address: shruti.saptarshi@jcu.edu.au.
authors.forename: Shruti R
authors.initials: SR
authors.lastname: Saptarshi
authors.forename: Michael F
authors.initials: MF
authors.lastname: Sharp
authors.forename: Sandip D
authors.initials: SD
authors.lastname: Kamath
authors.forename: Andreas L
authors.initials: AL
authors.lastname: Lopata
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003296:Cooking; D003429:Cross Reactions; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D005399:Fishes / Q000145:classification / Q000235:genetics / Q000276:immunology*; D006358:Hot Temperature; D010320:Parvalbumins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D010802:Phylogeny; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D017747:Seafood / Q000032:analysis
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal; D029941:Fish Proteins; D010320:Parvalbumins; D020033:Protein Isoforms
keywords: Allergen; Asia-Pacific fish; IgE epitopes; Monoclonal antibody; Parvalbumin; Seafood