title: Immunocapture of CD133-positive cells from human cancer cell lines by using monodisperse magnetic poly(glycidyl methacrylate) microspheres containing amino groups.
abstract: Magnetic poly(glycidyl methacrylate)-based macroporous microspheres with an average particle size of 4.2μm were prepared using a modified multi-step swelling polymerization method and by introducing amino functionality on their surfaces. Antibody molecules were oxidized on their carbohydrate moieties and bound to the amino-containing magnetic microspheres via a site-directed procedure. CD133-positive cells could be effectively captured from human cancer cell lines (HepG2, HCT116, MCF7, and IMR-32) by using magnetic microspheres conjugated to an anti-human CD133 antibody. After further culture, the immunocaptured CD133-expressing cells from IMR-32 proliferated and gradually detached from the magnetic microspheres. Flow-cytometric analysis confirmed the enrichment of CD133-expressing cells by using the antibody-bound magnetic microspheres. Such microspheres suitable for immunocapture are very promising for cancer diagnosis because the CD133-expressing cells in cancer cell lines have been suggested to be cancer stem cells.
authors.affiliation: Department of Chemical Engineering, Systems Biology and Tissue Engineering Research Center, National Chung Cheng University, Minhisung 621, Taiwan.
authors.forename: Wei-Chih
authors.initials: WC
authors.lastname: Kuan
authors.forename: Daniel
authors.initials: D
authors.lastname: Horák
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Plichta
authors.forename: Wen-Chien
authors.initials: WC
authors.lastname: Lee
mesh_terms: D000071916:AC133 Antigen; D000588:Amines / Q000737:chemistry*; D015703:Antigens, CD / Q000378:metabolism*; D002448:Cell Adhesion; D002455:Cell Division; D045744:Cell Line, Tumor; D048430:Cell Shape; D005434:Flow Cytometry; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D060328:Magnetic Phenomena; D008863:Microspheres; D010455:Peptides / Q000378:metabolism*; D058105:Polymerization; D011109:Polymethacrylic Acids / Q000737:chemistry*; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000071916:AC133 Antigen; D000588:Amines; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D011109:Polymethacrylic Acids; C042535:polyglycidyl methacrylate
keywords: Antibody; CD133-expressing cell; Magnetic; Microspheres; Poly(glycidyl methacrylate)