title: Enzyme-assisted extraction and ionic liquid-based dispersive liquid-liquid microextraction followed by high-performance liquid chromatography for determination of patulin in apple juice and method optimization using central composite design.
abstract: A simple and highly sensitive analytical methodology for isolation and determination of patulin in apple-juice samples, based on enzyme-assisted extraction (EAE) and ionic liquid-based dispersive liquid-liquid microextraction (IL-DLLME) was developed and optimized. Enzymes play essential roles in eliminating interference and increasing the extraction efficiency of patulin. Apple-juice samples were treated with pectinase and amylase. A mixture of 80 μL ionic liquid and 600 μL methanol (disperser solvent) was used for the IL-DLLME process. The sedimented phase was analyzed by high-performance liquid chromatography (HPLC). Experimental parameters controlling the performance of DLLME, were optimized using response surface methodology (RSM) based on central composite design (CCD). Under optimum conditions, the calibration curves showed high levels of linearity (R(2)>0.99) for patulin in the range of 1-200 ng g(-1). The relative standard deviation (RSD) for the seven analyses was 7.5%. The limits of detection (LOD) and limits of quantification (LOQ) were 0.15 ng g(-1) and 0.5 ng g(-1), respectively. The merit figures, compared with other methods, showed that new proposed method is an accurate, precise and reliable sample-pretreatment method that substantially reduces sample matrix interference and gives very good enrichment factors and detection limits for investigation trace amount of patulin in apple-juice samples.
authors.affiliation: Department of Food Science and Technology, Faculty of Nutrition Science, Food Science and Technology/National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: ab.mohammadi@sbmu.ac.ir.
authors.forename: Abdorreza
authors.initials: A
authors.lastname: Mohammadi
authors.forename: Rouya
authors.initials: R
authors.lastname: Tavakoli
authors.forename: Marzieh
authors.initials: M
authors.lastname: Kamankesh
authors.forename: Hamid
authors.initials: H
authors.lastname: Rashedi
authors.forename: Abdolmohammad
authors.initials: A
authors.lastname: Attaran
authors.forename: Mostafa
authors.initials: M
authors.lastname: Delavar
mesh_terms: D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D059627:Liquid Phase Microextraction; D027845:Malus / Q000737:chemistry*; D010365:Patulin / Q000032:analysis*
chemical_list: D010365:Patulin
keywords: Apple juice; Enzyme-assisted extraction; High-performance liquid chromatography; Ionic liquid-based dispersive liquid–liquid microextraction; Patulin; Response surface methodology