title: Allelic variation of killer cell immunoglobulin-like receptor 2DS5 impacts glycosylation altering cell surface expression levels.
abstract: Natural killer cell stimulatory receptor gene, KIR2DS5, is polymorphic. While KIR2DS5*002 is most frequently observed, other alleles have also been found. The proteins encoded by these alleles (KIR2DS5*002-*009) are expressed at varying levels on the surface of NKL and Jurkat transfectants. Gel electrophoresis of all allelic products showed two isoforms which differ in the extent of maturation of N-linked glycosylation. These isoforms differed in intensity and molecular weight among the allelic products. Site-directed mutagenesis was used to identify polymorphic variation at residues 123 and 157 as key in altering glycosylation and levels of surface expression.
authors.affiliation: Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20057, USA.
authors.forename: Noriko K
authors.initials: NK
authors.lastname: Steiner
authors.affiliation: Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20057, USA.
authors.forename: Sivanesan
authors.initials: S
authors.lastname: Dakshanamurthy
authors.affiliation: Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20057, USA.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Nguyen
authors.affiliation: Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20057, USA. Electronic address: hurleyc@georgetown.edu.
authors.forename: Carolyn Katovich
authors.initials: CK
authors.lastname: Hurley
mesh_terms: D000483:Alleles; D019295:Computational Biology; D016207:Cytokines / Q000378:metabolism; D003602:Cytotoxicity, Immunologic / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics; D006031:Glycosylation; D006801:Humans; D019169:Jurkat Cells; D007694:Killer Cells, Natural / Q000276:immunology*; D008213:Lymphocyte Activation / Q000235:genetics; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009154:Mutation / Q000235:genetics; D011110:Polymorphism, Genetic; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D054340:Receptors, KIR / Q000235:genetics / Q000378:metabolism*; D019076:Transgenes / Q000235:genetics
chemical_list: D016207:Cytokines; C531595:KIR2DS5 protein, human; D020033:Protein Isoforms; D054340:Receptors, KIR
keywords: 2DL; Cell surface molecules; Human; KIR; Killer cell immunoglobulin-like receptors; Natural killer cells; Polymorphism; killer cell Immunoglobulin-like receptor; two extracellular domains, long cytoplasmic tail