title: Application of a liquid chromatography-tandem mass spectrometry method to the pharmacokinetics, bioavailability and tissue distribution of neohesperidin dihydrochalcone in rats.
abstract: 1. This study was aimed at developing a high sensitive and selective liquid chromatography-tandem mass spectrometry method to quantify neohesperidin dihydrochalcone (NHDC) in rat plasma and tissues for pharmacokinetic, bioavailability and tissue distribution studies. 2. Biological samples were processed with one-step protein precipitation. Rutin was chosen as the internal standard (IS). Chromatographical separation was achieved on an SB-C18 (2.1 mm× 150 mm, 5 μm) column with acetonitrile--0.1% formic acid in water as the mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in negative ion mode; selected ion monitoring mode was used for quantification using target fragment ions m/z 611.4 for NHDC and m/z 609.1 for IS. 3. Calibration plots were linear over the range of 10-3000 ng/mL for NHDC. Lower limit of quantification (LLOQ) for NHDC was 10 ng/mL. Mean recovery of NHDC from plasma and tissues was better than 80.3%. Coefficient of variation of intra-day and inter-day precision were both less than 15%. The bioavailability of NHDC was 21.8%. 4. In conclusion, a sensitive, simple and specific LC-ESI-MS method for the determination of NHDC in rat biological samples was developed. This developed method is successfully used in the pharmacokinetic and tissue distribution study of NHDC in rats.
authors.affiliation: School of Pharmacy, Wenzhou Medical University , Wenzhou , People's Republic of China and.
authors.forename: Xianqin
authors.initials: X
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Pan
authors.forename: Ma
authors.initials: M
authors.lastname: Jianshe
authors.forename: Shaohua
authors.initials: S
authors.lastname: Shi
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Zheng
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xiang
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D001682:Biological Availability; D002138:Calibration; D047188:Chalcones / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D006569:Hesperidin / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D008297:Male; D017207:Rats, Sprague-Dawley; D012044:Regression Analysis; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D047188:Chalcones; C013613:neohesperidin dihydrochalcone; D006569:Hesperidin