title: AgarTrap: a simplified Agrobacterium-mediated transformation method for sporelings of the liverwort Marchantia polymorpha L.
abstract: The liverwort Marchantia polymorpha L. is being developed as an emerging model plant, and several transformation techniques were recently reported. Examples are biolistic- and Agrobacterium-mediated transformation methods. Here, we report a simplified method for Agrobacterium-mediated transformation of sporelings, and it is termed Agar-utilized Transformation with Pouring Solutions (AgarTrap). The procedure of the AgarTrap was carried out by simply exchanging appropriate solutions in a Petri dish, and completed within a week, successfully yielding sufficient numbers of independent transformants for molecular analysis (e.g. characterization of gene/protein function) in a single experiment. The AgarTrap method will promote future molecular biological study in M. polymorpha.
authors.affiliation: Center for Bioscience Research and Education, Utsunomiya University, Tochigi, 321-8505 Japan.
authors.forename: Shoko
authors.initials: S
authors.lastname: Tsuboyama
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kodama
mesh_terms: D060054:Agrobacterium / Q000254:growth & development / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D005821:Genetic Techniques; D047008:Marchantia / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D008856:Microscopy, Fluorescence; D016254:Mutagenesis, Insertional / Q000235:genetics; D030821:Plants, Genetically Modified; D013170:Spores; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial; C036483:T-DNA
keywords: AgarTrap; Agrobacterium tumefaciens; Bryophytes; Genetic transformation; Marchantia polymorpha; Rhizobium radiobactor