title: Soluble uridine diphospho-D-glucose: mycosporin glucosyltransferase from spores of Ascochyta fabae Speg.
abstract: The enzyme properties of a soluble uridine 5'-diphosphate (UDP) glucose: mycosporin-2 glucosyltransferase from spores of Ascochyta fabae Speg. (Fungi imperfecti) were studied. The optimal conditions for the glucose transfer from UDP-glucose to the mycosporin-2 (the amide form being the best acceptor) were determined; for maximal activity the glucosyltransferase requires a pH of about 8.5 and the presence of divalent cations (Mn(2+) being more efficient than Ca(2+) or Mg(2+)). The reaction was not reversible in presence of large amounts of UDP.
authors.affiliation: Départment de Biologie Végétale, Université C. Bernard, Lyon I, 43 Boulevard du 11 Novembre 1918, F-69622, Villeurbanne Cedex, France.
authors.forename: J L
authors.initials: JL
authors.lastname: Pittet
authors.forename: R
authors.initials: R
authors.lastname: Létoublon
authors.forename: J
authors.initials: J
authors.lastname: Frot-Coutaz
authors.forename: N
authors.initials: N
authors.lastname: Arpin