title: Effects of repeated applications of chlorimuron-ethyl on the soil microbial biomass, activity and microbial community in the greenhouse.
abstract: The impacts of repeated chlorimuron-ethyl applications on soil microbial community structure and function were studied under greenhouse conditions. Chlorimuron-ethyl was applied to soil samples at three different doses [1-,10-,100-fold of recommended field rate (T1, T10, T100)] for 3 years. The half-lives of chlorimuron-ethyl were 37.1-54.6 days. The soil microbial biomass (microbial biomass carbon and total phospholipid fatty acid), the microbial activity (basal respiration and average well color development), the ratio of Gram-negative/Gram-positive bacteria and Shannon index were stimulated by chlorimuron-ethyl during the initial period. Except for T100, the other treatments recovered to the untreated level. The ratio of fungi/bacteria decreased during the initial period and then recovered in the end. Principal component analysis of phospholipid fatty acid showed that chlorimuron-ethyl altered the microbial community structure. Except got T100, T1 and T10 were not different from the control at the end of experiment. These results suggested a dosage effect of chlorimuron-ethyl on the living microbial biomass and the microbial community.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Fengshou
authors.initials: F
authors.lastname: Dong
authors.forename: Xingang
authors.initials: X
authors.lastname: Liu
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Wu
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D018533:Biomass; D002244:Carbon / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005658:Fungi / Q000187:drug effects / Q000254:growth & development; D006540:Herbicides / Q000633:toxicity*; D011743:Pyrimidines / Q000633:toxicity*; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000633:toxicity*; D013453:Sulfonylurea Compounds / Q000633:toxicity*; D036401:Toxicity Tests, Chronic
chemical_list: D005227:Fatty Acids; D006540:Herbicides; D011743:Pyrimidines; D012987:Soil; D012989:Soil Pollutants; D013453:Sulfonylurea Compounds; D002244:Carbon; C112343:chlorimuron ethyl