title: Characterization of four midgut aminopeptidase N isozymes from Ostrinia furnacalis strains with different susceptibilities to Bacillus thuringiensis.
abstract: The full-length cDNA of four Ofapn aminopeptidases were cloned and sequenced from susceptible and resistant Ostrinia furnacalis strains. Four sequences were identified as APN because they shared the common structural features with APN from Lepidoptera, including the signal peptide, GPI anchor signal, the zinc binding/gluzincin motif HEX2HX18E and the gluzincin aminopeptidase motif GAMEN. Compared with APN sequences from the susceptible strain, there were 9, 5, 10 and 12 amino acid variations in the deduced protein sequences from the resistant strain. There were also differences in mRNA expression of the four Ofapn genes between resistant and susceptible O. furnacalis strains.
authors.affiliation: The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 West Yuanmingyuan Road, Beijing 100193, PR China; Newcastle Institute for Research on Environment and Sustainability, School of Biology, University of Newcastle, Newcastle Upon Tyne NE1 7RU, UK; Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, No. 40 South Nongke Road, Hefei 230031, PR China.
authors.forename: Lina
authors.initials: L
authors.lastname: Xu
authors.affiliation: The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 West Yuanmingyuan Road, Beijing 100193, PR China.
authors.forename: Zhenying
authors.initials: Z
authors.lastname: Wang
authors.affiliation: The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 West Yuanmingyuan Road, Beijing 100193, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Newcastle Institute for Research on Environment and Sustainability, School of Biology, University of Newcastle, Newcastle Upon Tyne NE1 7RU, UK; Environment and Life Sciences, College of Science and Technology, University of Salford, Salford M5 4WT, UK.
authors.forename: Natalie
authors.initials: N
authors.lastname: Ferry
authors.affiliation: Newcastle Institute for Research on Environment and Sustainability, School of Biology, University of Newcastle, Newcastle Upon Tyne NE1 7RU, UK.
authors.forename: Martin G
authors.initials: MG
authors.lastname: Edwards
authors.affiliation: Newcastle Institute for Research on Environment and Sustainability, School of Biology, University of Newcastle, Newcastle Upon Tyne NE1 7RU, UK.
authors.forename: Angharad M R
authors.initials: AM
authors.lastname: Gatehouse
authors.affiliation: The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 West Yuanmingyuan Road, Beijing 100193, PR China. Electronic address: kanglai.he@263.net.
authors.forename: Kanglai
authors.initials: K
authors.lastname: He
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001413:Bacillus thuringiensis; D018826:CD13 Antigens / Q000235:genetics*; D018076:DNA, Complementary / Q000032:analysis; D019476:Insect Proteins / Q000235:genetics; D007305:Insecticide Resistance / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics; D007915:Lepidoptera / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D010572:Pest Control, Biological / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D007527:Isoenzymes; D018826:CD13 Antigens
keywords: Aminopeptidase N; Bt receptors; Ostrinia furnacalis; cDNA; mRNA expression