title: EPs7630(®) from Pelargonium sidoides increases stress resistance in Caenorhabditis elegans probably via the DAF-16/FOXO pathway.
abstract: EPs7630(®) a water alcohol extract of the roots from Pelargonium sidoides contains several secondary metabolites including highly oxygenated coumarins, various phenolics and polyphenols. Using the DPPH assay to measure antioxidant activity a free radical scavenging activity of 14.7±0.85μg/ml (IC50) was determined. As an in vivo model Caenorhabditis elegans was applied to study the effect of EPs7630(®) on stress resistance. EPs7630(®) treatment reduces intracellular hsp-16.2::GFP expression (induced by the pro-oxidant juglone) indicating that the secondary metabolites of EPs7630(®) are bioavailable and exhibit antioxidant activities in vivo. Application of EPs7630(®) (50μg/ml) to the transgenic mutant TJ356 induced the migration of the transcription factor DAF-16 from cytosol to the nucleus, suggesting a prominent role of DAF-16/FOXO in the daf-2 pathway for stress resistance.
authors.affiliation: Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, INF 364, 69120 Heidelberg, Germany.
authors.forename: Leila
authors.initials: L
authors.lastname: Rezaizadehnajafi
authors.affiliation: Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, INF 364, 69120 Heidelberg, Germany. Electronic address: wink@uni-hd.de.
authors.forename: Michael
authors.initials: M
authors.lastname: Wink
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism; D051858:Forkhead Transcription Factors; D009285:Naphthoquinones; D018384:Oxidative Stress / Q000187:drug effects*; D031316:Pelargonium; D010936:Plant Extracts / Q000494:pharmacology*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D029742:Caenorhabditis elegans Proteins; C476332:EPs 7630; D051858:Forkhead Transcription Factors; D009285:Naphthoquinones; D010936:Plant Extracts; D014157:Transcription Factors; C108831:daf-16 protein, C elegans; C005134:juglone
keywords: Caenorhabditis elegans; DAF-16; Pelargonium sidoides; Stress resistance