title: Cytotoxicity of ICD-85 NPs on Human Cervical Carcinoma HeLa Cells through Caspase-8 Mediated Pathway.
abstract: The biological application of nanoparticles (NPs) is a rapidly developing area of nanotechnology that raises new possibilities in the treatment of human cancers. The cytotoxicity was evaluated by MTT and LDH assays. The apoptotic effect of free ICD-85 and ICD-85 NPs on HeLa cells was assessed using caspase-8 colorimetric assay. The MTT assay showed that ICD-85 NPs could enhance the in-vitro cytotoxicity against HeLa cells compared to the free ICD-85. The IC50 value at 72 h was reduced from 25 ± 2.9 μg/mL for free ICD-85 to 15.5 ± 2.4 μg/mL for ICD-85 NPs. However, LDH assay demonstrated that ICD-85 has dose-dependent cytotoxicity on HeLa cells while ICD-85 NPs exhibited weaker cytotoxicity on same cells. The results also indicate that ICD-85-induced apoptosis on HeLa cells is associated with the activation of caspase-8. Moreover, caspase-8 assay analysis demonstrated that the ICD- 85 NPs induced a higher apoptotic rate in HeLa cells compared to free ICD-85. Our results demonstrated that the encapsulation of ICD-85 enhances its anti-proliferative effects. Taken together, these results suggest that the delivery of ICD-85 in nanoparticles may be a promising approach for the treatment of the cancer.
authors.affiliation: Department of Biochemistry, Faculty of Basic Sciences, Payame Noor University, Tehran, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Moradhaseli
authors.forename: Abbas
authors.initials: A
authors.lastname: Zare Mirakabadi
authors.forename: Ali
authors.initials: A
authors.lastname: Sarzaeem
authors.forename: Morteza
authors.initials: M
authors.lastname: Kamalzadeh
authors.forename: Reza
authors.initials: R
authors.lastname: Haji Hosseini
keywords: Cancer; Caspase-8; HeLa cell line; ICD-85; MTT assay; Nanoparticles