title: Liquid chromatography tandem mass spectrometry method for determination of antidiabetic chalcones derivative S001-469 in rat plasma, urine and feces: application to pharmacokinetic study.
abstract: A sensitive and selective liquid chromatography tandem mass spectrometry assay was developed for quantitation of a novel antidiabetic chalcones derivative S001-469 in rat matrices. Plasma and urine samples were prepared by double liquid-liquid extraction with diethyl ether and feces by protein precipitation using acetonitrile. Chromatographic elution was carried on cyano guard column (30 mm × 4.6 mm i.d., 5 µm) in isocratic mode at a flow rate of 0.75 mL/min using mobile phase comprising of methanol: ammonium acetate buffer (pH 4.6, 10 mM) (90:10, v/v). Run time was 6 min. Detection was achieved by employing positive ionization mode on a triple-quadrupole LC-MS/MS system with an electrospray ionization (ESI) source. The calibration curves were linear over the range of 0.78-400 ng/mL for all 3 matrices. The method was validated and proved reliable through high and consistent intra- and inter- day accuracy and precision (<15%) values. Recoveries was >85% from spiked plasma, urine and feces samples. S001-469 was stable in plasma at room temperature till 8 h and at -60 °C for 30 d and 3 freeze-thaw cycles.
authors.affiliation: Pharmacokinetics and Metabolism Division, CSIR-Central Drug Research Institute, Lucknow, India.
authors.forename: N
authors.initials: N
authors.lastname: Gautam
authors.affiliation: Pharmacokinetics and Metabolism Division, CSIR-Central Drug Research Institute, Lucknow, India.
authors.forename: R P
authors.initials: RP
authors.lastname: Singh
authors.affiliation: Pharmacokinetics and Metabolism Division, CSIR-Central Drug Research Institute, Lucknow, India.
authors.forename: H N
authors.initials: HN
authors.lastname: Kushwaha
authors.affiliation: Pharmacokinetics and Metabolism Division, CSIR-Central Drug Research Institute, Lucknow, India.
authors.forename: A
authors.initials: A
authors.lastname: Misra
authors.affiliation: Pharmacokinetics and Metabolism Division, CSIR-Central Drug Research Institute, Lucknow, India.
authors.forename: H
authors.initials: H
authors.lastname: Kumar
authors.affiliation: Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow, India.
authors.forename: P C
authors.initials: PC
authors.lastname: Verma
authors.affiliation: Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow, India.
authors.forename: R
authors.initials: R
authors.lastname: Pratap
authors.affiliation: Pharmacokinetics and Metabolism Division, CSIR-Central Drug Research Institute, Lucknow, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Singh
mesh_terms: D000818:Animals; D047188:Chalcones / Q000097:blood / Q000737:chemistry* / Q000493:pharmacokinetics* / Q000652:urine; D002853:Chromatography, Liquid / Q000379:methods; D005243:Feces; D007004:Hypoglycemic Agents / Q000097:blood / Q000737:chemistry* / Q000493:pharmacokinetics* / Q000652:urine; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D047188:Chalcones; D007004:Hypoglycemic Agents