title: Diterpenoid alkaloids and flavonoids from Delphinium trichophorum.
abstract: Five hetisane-type C20-diterpenoid alkaloids, trichodelphinines A-E, one delnudine-type C20-diterpenoid alkaloid, trichodelphinine F and three known flavonoids, quercetin, quercetin 3-O-β-D-glucopyranoside, and quercetin 3-O-β-D-glucopyranoside-7-O-α-L-arabinopyranoside, were isolated from whole plants of Delphinium trichophorum Franch. Their structures were elucidated on the basis of extensive spectroscopic analysis, including HSQC, HMBC, (1)H-(1)H COSY, NOESY and X-ray crystallographic analysis, and from chemical evidence. The cytotoxic activities of the diterpenoid alkaloids were evaluated using the MTT method, and the IC50 values of their cytotoxicity against A549 cancer cells ranged from 12.03 to 52.79 μM.
authors.affiliation: Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China.
authors.forename: Chao-Zhan
authors.initials: CZ
authors.lastname: Lin
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.
authors.forename: Zhong-Xiang
authors.initials: ZX
authors.lastname: Zhao
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.
authors.forename: Si-Min
authors.initials: SM
authors.lastname: Xie
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.
authors.forename: Ju-Hua
authors.initials: JH
authors.lastname: Mao
authors.affiliation: Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China. Electronic address: zhuchenchen@vip.sina.com.
authors.forename: Chen-Chen
authors.initials: CC
authors.lastname: Zhu
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Li
authors.affiliation: Tibetan Traditional Medical College, Lasa 850000, PR China.
authors.forename: Bairi
authors.initials: B
authors.lastname: Zeren-dawa
authors.affiliation: Tibetan Traditional Medical College, Lasa 850000, PR China.
authors.forename: Kangsa
authors.initials: K
authors.lastname: Suolang-qimei
authors.affiliation: Tibetan Traditional Medical College, Lasa 850000, PR China.
authors.forename: Dun
authors.initials: D
authors.lastname: Zhu
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.
authors.forename: Tian-Qin
authors.initials: TQ
authors.lastname: Xiong
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.
authors.forename: Ai-Zhi
authors.initials: AZ
authors.lastname: Wu
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018360:Crystallography, X-Ray; D031882:Delphinium / Q000737:chemistry*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D020128:Inhibitory Concentration 50; D008968:Molecular Conformation; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011794:Quercetin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D018609:Tibet
chemical_list: D000470:Alkaloids; D000970:Antineoplastic Agents; D004224:Diterpenes; D005419:Flavonoids; D005960:Glucosides; D011794:Quercetin; C110309:quercetin 3-O-glucopyranoside
keywords: Delnudine-type diterpenoid alkaloid; Delphinium trichophorum Franch; Diterpenoid alkaloid; Hetisane-type diterpenoid alkaloid; KRXFYFOHIOSSNE-ZXMOXNTFSA-N; Ranunculaceae; ULBJMFAWRDNRAM-JCTIUFQSSA-N; VPGOXBOVBANQMB-OQWYUALGSA-N; WDOZPTBXHDZRAC-GJGFCZQESA-N; WNZAQACNVGQHRH-NRNHFJSPSA-N; YPJJUKPGIAXPIU-RAXXLDHPSA-N