title: Construction of integrated linkage map of a recombinant inbred line population of white lupin (Lupinus albus L.).
abstract: We report the development of a Diversity Arrays Technology (DArT) marker panel and its utilisation in the development of an integrated genetic linkage map of white lupin (Lupinus albus L.) using an F8 recombinant inbred line population derived from Kiev Mutant/P27174. One hundred and thirty-six DArT markers were merged into the first genetic linkage map composed of 220 amplified fragment length polymorphisms (AFLPs) and 105 genic markers. The integrated map consists of 38 linkage groups of 441 markers and spans a total length of 2,169 cM, with an average interval size of 4.6 cM. The DArT markers exhibited good genome coverage and were associated with previously identified genic and AFLP markers linked with quantitative trait loci for anthracnose resistance, flowering time and alkaloid content. The improved genetic linkage map of white lupin will aid in the identification of markers for traits of interest and future syntenic studies.
authors.affiliation: Graham Centre for Agricultural Innovation (an alliance between NSW Department of Primary Industries and Charles Sturt University), Wagga Wagga Agricultural Institute , Wagga Wagga, NSW 2650 , Australia.
authors.forename: Cina Ann
authors.initials: CA
authors.lastname: Vipin
authors.forename: David J
authors.initials: DJ
authors.lastname: Luckett
authors.forename: John D I
authors.initials: JD
authors.lastname: Harper
authors.forename: Gavin J
authors.initials: GJ
authors.lastname: Ash
authors.forename: Andrzej
authors.initials: A
authors.lastname: Kilian
authors.forename: Simon R
authors.initials: SR
authors.lastname: Ellwood
authors.forename: Huyen T T
authors.initials: HT
authors.lastname: Phan
authors.forename: Harsh
authors.initials: H
authors.lastname: Raman
keywords: Diversity Array Technology; Lupinus albus; genetic linkage mapping; molecular markers