title: Glucose elevates NITRATE TRANSPORTER2.1 protein levels and nitrate transport activity independently of its HEXOKINASE1-mediated stimulation of NITRATE TRANSPORTER2.1 expression.
abstract: Mineral nutrient uptake and assimilation is closely coordinated with the production of photosynthate to supply nutrients for growth. In Arabidopsis (Arabidopsis thaliana), nitrate uptake from the soil is mediated by genes encoding high- and low-affinity transporters that are transcriptionally regulated by both nitrate and photosynthate availability. In this study, we have studied the interactions of nitrate and glucose (Glc) on gene expression, nitrate transport, and growth using glucose-insensitive2-1 (gin2-1), which is defective in sugar responses. We confirm and extend previous work by showing that HEXOKINASE1-mediated oxidative pentose phosphate pathway (OPPP) metabolism is required for Glc-mediated NITRATE TRANSPORTER2.1 (NRT2.1) expression. Treatment with pyruvate and shikimate, two products derived from intermediates of the OPPP that are destined for amino acid production, restores wild-type levels of NRT2.1 expression, suggesting that metabolites derived from OPPP metabolism can, together with Glc, directly stimulate high levels of NRT2.1 expression. Nitrate-mediated NRT2.1 expression is not influenced by gin2-1, showing that Glc does not influence NRT2.1 expression through nitrate-mediated mechanisms. We also show that Glc stimulates NRT2.1 protein levels and transport activity independently of its HEXOKINASE1-mediated stimulation of NRT2.1 expression, demonstrating another possible posttranscriptional mechanism influencing nitrate uptake. In gin2-1 plants, nitrate-responsive biomass growth was strongly reduced, showing that the supply of OPPP metabolites is essential for assimilating nitrate for growth.
authors.affiliation: Cell and Developmental Biology Department, John Innes Centre, Norwich NR4 7UH, United Kingdom.
authors.forename: Femke
authors.initials: F
authors.lastname: de Jong
authors.forename: Kate
authors.initials: K
authors.lastname: Thodey
authors.forename: Laurence V
authors.initials: LV
authors.lastname: Lejay
authors.forename: Michael W
authors.initials: MW
authors.lastname: Bevan
mesh_terms: D000641:Ammonia / Q000378:metabolism; D027321:Anion Transport Proteins / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D018506:Gene Expression Regulation, Plant; D005947:Glucose / Q000378:metabolism*; D006593:Hexokinase / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009566:Nitrates / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D010427:Pentose Phosphate Pathway; D030821:Plants, Genetically Modified; D019289:Pyruvic Acid / Q000378:metabolism / Q000494:pharmacology; D012765:Shikimic Acid / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000641:Ammonia; D027321:Anion Transport Proteins; D029681:Arabidopsis Proteins; D005947:Glucose; D006593:Hexokinase; D009566:Nitrates; D009584:Nitrogen; D019289:Pyruvic Acid; D012765:Shikimic Acid; C477335:NRT2.1 protein, Arabidopsis; C473630:ATHXK1 protein, Arabidopsis