title: λ Phage nanobioparticle expressing apoptin efficiently suppress human breast carcinoma tumor growth in vivo.
abstract: Using phages is a novel field of cancer therapy and phage nanobioparticles (NBPs) such as λ phage could be modified to deliver and express genetic cassettes into eukaryotic cells safely in contrast with animal viruses. Apoptin, a protein from chicken anemia virus (CAV) has the ability to specifically induce apoptosis only in carcinoma cells. We presented a safe method of breast tumor therapy via the apoptin expressing λ NBPs. Here, we constructed a λ ZAP-CMV-apoptin recombinant NBP and investigated the effectiveness of its apoptotic activity on BT-474, MDA-MB-361, SKBR-3, UACC-812 and ZR-75 cell lines that over-expressing her-2 marker. Apoptosis was evaluated via annexin-V fluorescent iso-thiocyanate/propidium iodide staining, flow-cytometric method and TUNEL assay. Transfection with NBPs carrying λ ZAP-CMV-apoptin significantly inhibited growth of all the breast carcinoma cell lines in vitro. Also nude mice model implanted BT-474 human breast tumor was successfully responded to the systemic and local injection of untargeted recombinant λ NBPs. The results presented here reveal important features of recombinant λ nanobioparticles to serve as safe delivery and expression platform for human cancer therapy.
authors.affiliation: Applied Cell Sciences Department, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran ; Research Center for Science and Technology in Medicine (RCSTiM), Tehran University of Medical Sciences (TUMS), Tehran, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Shoae-Hassani
authors.forename: Peyman
authors.initials: P
authors.lastname: Keyhanvar
authors.forename: Alexander Marcus
authors.initials: AM
authors.lastname: Seifalian
authors.forename: Seyed Abdolreza
authors.initials: SA
authors.lastname: Mortazavi-Tabatabaei
authors.forename: Narmin
authors.initials: N
authors.lastname: Ghaderi
authors.forename: Khosro
authors.initials: K
authors.lastname: Issazadeh
authors.forename: Nour
authors.initials: N
authors.lastname: Amirmozafari
authors.forename: Javad
authors.initials: J
authors.lastname: Verdi
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents; D010582:Bacteriophage lambda / Q000235:genetics*; D001943:Breast Neoplasms / Q000473:pathology*; D036022:Capsid Proteins / Q000235:genetics*; D002455:Cell Division / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D053758:Nanoparticles; D011994:Recombinant Proteins / Q000235:genetics
chemical_list: D000970:Antineoplastic Agents; D036022:Capsid Proteins; D011994:Recombinant Proteins; C091717:VP3 protein, Chicken anemia virus