title: [Xuefu zhuyu decoction containing serum in vitro induced expressions of desmin and alpha-actin: an experimental research].
abstract: OBJECTIVE
  To observe whether Xuefu Zhuyu Decoction (XZD) could induce the differentiation of mesenchymal stem cells (MSCs) into cardiac myoid cells, thus seeking for safe and effective inducers.
  
  
  METHODS
  The serum pharmacological method was used to induce. XZD containing serum was prepared. MSCs were isolated and cultured. The serum cytotoxicity was detected by MTT. The third generation of favorably grown cells was selected in this experiment. Cells were divided into three groups, i.e., the vehicle control group, the XZD containing serum induced group, and the 5-azacytidine induced group. Expressions of Desmin and alpha-actin were detected by immunocytochemical staining method.
  
  
  RESULTS
  Before induction protein expressions of Desmin and alpha-actin were negative, and few was weakly positive. There was no statistical difference in the weak positive expression rate among the 3 groups (P > 0.05). After induction protein expressions of Desmin and alpha-actin were negative, and few was weakly positive in the vehicle control group. Protein expressions of Desmin and alpha-actin were positive in the XZC containing serum induced group and the 5-azacytidine induced group. There was statistical difference in the positive expression rate when compared with the vehicle control group (P > 0.05).
  
  
  CONCLUSIONS
  XZD played a role in in vitro inducing differentiation MSCs to cardiac myoid cells. It might participate in expressions of Desmin and alpha-actin.
authors.affiliation: Basic Medical College of Shandong University of Traditional Chinese Medicine, Shandong 250355, China. myx1008@126.com
authors.forename: Yue-Xiang
authors.initials: YX
authors.lastname: Ma
authors.forename: Li-Qing
authors.initials: LQ
authors.lastname: Liu
authors.forename: Li-Mei
authors.initials: LM
authors.lastname: Qin
authors.forename: Guang-Min
authors.initials: GM
authors.lastname: Zuo
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D003893:Desmin / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D044967:Serum
chemical_list: D000199:Actins; D003893:Desmin; D004365:Drugs, Chinese Herbal; C513826:Xue-Fu-Zhu-Yu decoction