title: Biocompatibility of a novel cyanoacrylate based tissue adhesive: cytotoxicity and biochemical property evaluation.
abstract: Cyanoacrylate (CA) is most widely used as a medical and commercial tissue adhesive because of easier wound closure, good cosmetic results and little discomfort. But, CA-based tissue adhesives have some limitations including the release of cytotoxic chemicals during biodegradation. In previous study, we made prepolymerized allyl 2-CA (PACA) based tissue adhesive, resulting in longer chain structure. In this study, we investigated a biocompatibility of PACA as alternative tissue adhesive for medical application, comparing with that of Dermabond® as commercial tissue adhesive. The biocompatibility of PACA was evaluated for short-term (24 hr) and long-term (3 and 7 days) using conventional cytotoxicity (WST, neutral red, LIVE/DEAD and TUNEL) assays, hematoxylin-eosin (H&E) and Masson trichrome (MT) staining. Besides we examined the biochemical changes in cells and DNA induced by PACA and Dermabond® utilizing Raman spectroscopy which could observe the denaturation and conformational changes in protein, as well as disintegration of the DNA/RNA by cell death. In particular, we analyzed Raman spectrum using the multivariate statistical methods including principal component analysis (PCA) and support vector machine (SVM). As a result, PACA and Dermabond® tissue adhesive treated cells and tissues showed no difference of the cell viability values, histological analysis and Raman spectral intensity. Also, the classification analysis by means of PCA-SVM classifier could not discriminate the difference between the PACA and Dermabond® treated cells and DNA. Therefore we suggest that novel PACA might be useful as potential tissue adhesive with effective biocompatibility.
authors.affiliation: Department of Biomedical Engineering and Healthcare Industry Research Institute, Kyung Hee University, Seoul, Korea.
authors.forename: Young Ju
authors.initials: YJ
authors.lastname: Lee
authors.forename: Gyeong Bok
authors.initials: GB
authors.lastname: Jung
authors.forename: Samjin
authors.initials: S
authors.lastname: Choi
authors.forename: Gihyun
authors.initials: G
authors.lastname: Lee
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Kim
authors.forename: Ho Sung
authors.initials: HS
authors.lastname: Son
authors.forename: Hyunsu
authors.initials: H
authors.lastname: Bae
authors.forename: Hun-Kuk
authors.initials: HK
authors.lastname: Park
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003487:Cyanoacrylates / Q000009:adverse effects / Q000737:chemistry*; D008297:Male; D051379:Mice; D025341:Principal Component Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013059:Spectrum Analysis, Raman; D060388:Support Vector Machine; D014014:Tissue Adhesives / Q000009:adverse effects* / Q000737:chemistry
chemical_list: D003487:Cyanoacrylates; D014014:Tissue Adhesives