title: Proteomic investigation of response to FORL infection in tomato roots.
abstract: Fusarium oxysporum f. sp. radicis-lycopersici (FORL) leading to fusarium crown and root rot is considered one of the most destructive tomato soilborne diseases occurring in greenhouse and field crops. In this study, response to FORL infection in tomato roots was investigated by differential proteomics in susceptible (Monalbo) and resistant (Momor) isogenic tomato lines, thus leading to identify 33 proteins whose amount changed depending on the pathogen infection, and/or on the two genotypes. FORL infection induced accumulation of pathogen-related proteins (PR proteins) displaying glucanase and endochitinases activity or involved in redox processes in the Monalbo genotype. Interestingly, the level of the above mentioned PR proteins was not influenced by FORL infection in the resistant tomato line, while other proteins involved in general response mechanisms to biotic and/or abiotic stresses showed significant quantitative differences. In particular, the increased level of proteins participating to arginine metabolism and glutathione S-transferase (GST; EC 2.5.1.18) as well as that of protein LOC544002 and phosphoprotein ECPP44-like, suggested their key role in pathogen defence.
authors.affiliation: Proteomic and Biomolecular Mass Spectrometry Center, Institute of Food Sciences, Italian National Research Council (CNR), Via Roma 64 a/c, 83100 Avellino, Italy.
authors.forename: Maria Fiorella
authors.initials: MF
authors.lastname: Mazzeo
authors.affiliation: Proteomic and Biomolecular Mass Spectrometry Center, Institute of Food Sciences, Italian National Research Council (CNR), Via Roma 64 a/c, 83100 Avellino, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Cacace
authors.affiliation: Department of Agricultural Sciences, University of Naples 'Federico II', Via Università 100, 80055 Portici, NA, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Ferriello
authors.affiliation: Department of Sustainable Agro-Ecosystems and Bioresources, Fondazione Edmund Mach, Via E. Mach 1, 38010 S. Michele all'Adige, TN, Italy.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Puopolo
authors.affiliation: Department of Agricultural Sciences, University of Naples 'Federico II', Via Università 100, 80055 Portici, NA, Italy.
authors.forename: Astolfo
authors.initials: A
authors.lastname: Zoina
authors.affiliation: Department of Agricultural Sciences, University of Naples 'Federico II', Via Università 100, 80055 Portici, NA, Italy.
authors.forename: Maria Raffaella
authors.initials: MR
authors.lastname: Ercolano
authors.affiliation: Proteomic and Biomolecular Mass Spectrometry Center, Institute of Food Sciences, Italian National Research Council (CNR), Via Roma 64 a/c, 83100 Avellino, Italy. Electronic address: rsiciliano@isa.cnr.it.
authors.forename: Rosa Anna
authors.initials: RA
authors.lastname: Siciliano
mesh_terms: D005670:Fusarium / Q000472:pathogenicity*; D005838:Genotype; D018551:Solanum lycopersicum / Q000235:genetics / Q000382:microbiology*; D010935:Plant Diseases; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000382:microbiology*; D040901:Proteomics
chemical_list: D010940:Plant Proteins
keywords: 2-DE; 2-DE maps; FORL; FORL infection; Fusarium oxysporum f. sp. radicis-lycopersici; GST; IEF; MALDI-TOF-MS; MS/MS; PR proteins; Proteomics; SDS-PAGE; Tomato; collision induced dissociation experiments; electrospray ionization tandem mass spectrometry coupled with nano-reverse phase liquid chromatography; glutathione S-transferase (EC 2.5.1.18); isoelectric focusing; m/z; mass/charge; matrix assisted laser desorption ionization-time of flight-mass spectrometry; nano-HPLC-ESI-MS/MS; pathogenesis-related proteins; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; two-dimensional electrophoresis; two-dimensional electrophoretic maps