title: Autophagy mediates anti-melanogenic activity of 3'-ODI in B16F1 melanoma cells.
abstract: Autophagy is a cellular degradation process for cellular aggregates and unneeded cellular compartments including damaged mitochondria, ER, and peroxisomes. Melanosome is cellular organelle that is the cellular site of generation, storage and transports of melanin in melanocytes. Despite potential importance of autophagy, the role of autophagy in melanogenesis and melanosome autophagy are largely unknown. In here, we identified 3'-hydroxydaidzein (3'-ODI) as an autophagy inducer from a phytochemical library screening. Treatment with 3'-ODI significantly reduced α-MSH-mediated melanogenesis but efficiently increased autophagy both in melanoma cells and melanocytes. Furthermore, inhibition of autophagy significantly reduced the anti-melanogenic effects of 3'-ODI in α-MSH-stimulated melanoma cells. Taken together, these results suggest that autophagy mediates anti-melanogenic activity of 3'-ODI.
authors.affiliation: Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Eun Sung
authors.initials: ES
authors.lastname: Kim
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Shin
authors.forename: Su Hyeon
authors.initials: SH
authors.lastname: Seok
authors.forename: Jun Bum
authors.initials: JB
authors.lastname: Kim
authors.forename: Huikyoung
authors.initials: H
authors.lastname: Chang
authors.forename: So Jung
authors.initials: SJ
authors.lastname: Park
authors.forename: Yoon Kyung
authors.initials: YK
authors.lastname: Jo
authors.forename: Eun Sun
authors.initials: ES
authors.lastname: Choi
authors.forename: Jun-Seong
authors.initials: JS
authors.lastname: Park
authors.forename: Myeong Hun
authors.initials: MH
authors.lastname: Yeom
authors.forename: Chol Seung
authors.initials: CS
authors.lastname: Lim
authors.forename: Dong-Hyung
authors.initials: DH
authors.lastname: Cho
mesh_terms: D000818:Animals; D001343:Autophagy / Q000187:drug effects* / Q000235:genetics; D000071187:Autophagy-Related Protein 5; D045744:Cell Line, Tumor; D007529:Isoflavones / Q000494:pharmacology*; D008543:Melanins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D008544:Melanocytes / Q000187:drug effects* / Q000378:metabolism; D020460:Melanosomes / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D034622:RNA Interference; D000521:alpha-MSH / Q000494:pharmacology
chemical_list: C538958:3'-hydroxydaidzein; C495773:Atg5 protein, mouse; D000071187:Autophagy-Related Protein 5; D007529:Isoflavones; D008543:Melanins; D008869:Microtubule-Associated Proteins; D000521:alpha-MSH
keywords: 3′-ODI; Autophagy; B16F1 cells; Melanogenesis; α-MSH