title: Production of stem cell factor in canine mast cell tumors.
abstract: Mast cell tumor (MCT) is the most common cutaneous tumor in dogs. We recently revealed that production of stem cell factor (SCF) contributes to the proliferation of neoplastic mast cells in an autocrine/paracrine manner. The aim of the present study was to determine the contribution of the mechanism in clinical MCTs. In consequence, high SCF expression (>10 times compared to HRMC cells) was observed in 5 of 7 MCT samples used in the study regardless of KIT mutation, which was confirmed in immunohistochemical analysis. In addition, production of SCF was observed in Ki-67-positive cells in the MCT xenograft. These results indicate the broad contribution of SCF autocrine/paracrine mechanism on clinical MCTs, providing the rationale for the clinical use of KIT inhibitors regardless of KIT mutation.
authors.affiliation: Cooperative Major in Advanced Health Science, Graduate School of Bio-Applications and System Engineering, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Amagai
authors.affiliation: Cooperative Major in Advanced Health Science, Graduate School of Bio-Applications and System Engineering, Tokyo University of Agriculture and Technology, Tokyo, Japan; Laboratories of Comparative Animal Medicine, Division of Animal Life Science, Tokyo University of Agriculture and Technology, Tokyo, Japan. Electronic address: akane@cc.tuat.ac.jp.
authors.forename: Akane
authors.initials: A
authors.lastname: Tanaka
authors.affiliation: Cooperative Major in Advanced Health Science, Graduate School of Bio-Applications and System Engineering, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Kyungsook
authors.initials: K
authors.lastname: Jung
authors.affiliation: Laboratories of Veterinary Molecular Pathology and Therapeutics, Division of Animal Life Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Matsuda
authors.affiliation: Cooperative Major in Advanced Health Science, Graduate School of Bio-Applications and System Engineering, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Oida
authors.affiliation: Cooperative Major in Advanced Health Science, Graduate School of Bio-Applications and System Engineering, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Sho
authors.initials: S
authors.lastname: Nishikawa
authors.affiliation: Cooperative Major in Advanced Health Science, Graduate School of Bio-Applications and System Engineering, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Hyosun
authors.initials: H
authors.lastname: Jang
authors.affiliation: Cooperative Major in Advanced Health Science, Graduate School of Bio-Applications and System Engineering, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Saori
authors.initials: S
authors.lastname: Ishizaka
authors.affiliation: Cooperative Major in Advanced Health Science, Graduate School of Bio-Applications and System Engineering, Tokyo University of Agriculture and Technology, Tokyo, Japan; Laboratories of Veterinary Molecular Pathology and Therapeutics, Division of Animal Life Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000235:genetics / Q000378:metabolism*; D004285:Dogs; D005260:Female; D005434:Flow Cytometry; D007150:Immunohistochemistry / Q000662:veterinary; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D012334:RNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012878:Skin Neoplasms / Q000235:genetics / Q000378:metabolism*; D019089:Stem Cell Factor / Q000235:genetics / Q000378:metabolism*
chemical_list: D012334:RNA, Neoplasm; D019089:Stem Cell Factor
keywords: KIT; Mast cell tumor; Stem cell factor