title: Genetic polymorphisms of the IFNλ genes are associated with biochemical features in Han Chinese with HCV infection from Yunnan Province, China.
abstract: Hepatitis C virus (HCV) is the pathogenic factor for hepatitis C disease, which could lead to chronic or serious hepatic diseases. Previous studies have identified that the IL28B gene polymorphisms were associated with therapeutic effect and viral clearness of HCV patients. We aimed to test whether genetic polymorphisms of three IFNλ genes (IL28A, IL28B and IL29) are associated with HCV infection in Han Chinese. We collected whole blood of 261 HCV infectious patients without any therapy and 265 matched normal controls from Yunnan Province. Among these subjects, 28.4% (74/261) of HCV patients and 26.8% (71/265) of controls were male. Ten SNPs (rs8099917, rs10853728, rs11883177, rs12980602, rs4803224, rs11671087, rs11665818, rs8108008, rs7248931, and rs30461), which covered the whole region of the IL28A, IL28B, and IL29 genes, were genotyped. Our results showed that there was no association between genotypes and alleles of the IFNλ gene polymorphisms and HCV infection. One haplotype (TGCTGTGGAT), which was consisted of ten SNPs, showed a significantly higher frequency in HCV patients (11/522 = 2.1%) than in controls (1/530 = 0.2%) (P = 0.003). We performed association analyses for biochemical features and genotype of each SNP, and found that HCV patients with certain genotypes of some SNPs had a higher level of the ALT/AST ratio and total blood bilirubin (TBIL) compared to healthy controls. Our results suggested the IFNλ gene polymorphisms might be associated with clinical features of HCV patients from Yunnan Province, China.
authors.affiliation: Molecular Virus Units, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China. Electronic address: zam1980@yeah.net.
authors.forename: A-Mei
authors.initials: AM
authors.lastname: Zhang
authors.affiliation: Molecular Virus Units, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Ma
authors.affiliation: Molecular Virus Units, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China; Engineering Research Center of Molecular Diagnosis, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Song
authors.affiliation: Molecular Virus Units, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
authors.forename: Binghui
authors.initials: B
authors.lastname: Wang
authors.affiliation: Molecular Virus Units, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Molecular Virus Units, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Molecular Virus Units, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China. Electronic address: oliverxia2000@aliyun.com.cn.
authors.forename: Xueshan
authors.initials: X
authors.lastname: Xia
mesh_terms: D001663:Bilirubin / Q000097:blood; D002681:China; D005260:Female; D056726:Genetic Association Studies; D006239:Haplotypes; D016174:Hepacivirus / Q000302:isolation & purification / Q000378:metabolism; D006526:Hepatitis C / Q000235:genetics* / Q000473:pathology / Q000821:virology; D006801:Humans; D007372:Interferons; D007378:Interleukins / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide; D014764:Viral Proteins / Q000378:metabolism; D000095022:Interferon Lambda
chemical_list: D001663:Bilirubin; D007372:Interferons; D007378:Interleukins; D014764:Viral Proteins; C000624353:interferon-lambda, human; D000095022:Interferon Lambda
keywords: Biochemical features; Chinese; HCV infection; IFNλ genes; Variations