title: In vitro antioxidant activities of the polysaccharides from Pleurotus tuber-regium (Fr.) Sing.
abstract: The antioxidant activities of two homogeneous polysaccharides, water extraction of polysaccharide (W-PTR) and alkali extraction of polysaccharide (A-PTR) from Pleurotus tuber-regium (Fr.) Sing. were evaluated. Results showed that W-PTR was stronger than A-PTR in O₂(-·) scavenging activity, while A-PTR was stronger than W-PTR in the scavenging activities to ·OH, DPPH·, inhibition effects on liver lipid peroxidation, liver mitochondria swelling, and red blood cell (RBC) hemolysis. At concentration of 0.3 g/L, the O₂(-·) scavenging activity of W-PTR and A-PTR were 85.7% and 71.7%. At concentration of 0.25 g/L, the ·OH scavenging activity of W-PTR and A-PTR were 64.20% and 74.97%. At the concentration of 0.3 g/L, the lipid peroxidation inhibition effects of A-PTR were 79.37%. At concentration of 0.8 g/L, the hemolysis inhibition rate of A-PTR was 77.16%. The polysaccharides of P. tuber-regium had strong antioxidant potency and might be exploited as effective natural antioxidant to alleviate oxidative stress.
authors.affiliation: Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, China.
authors.forename: Guang-Hong
authors.initials: GH
authors.lastname: Wu
authors.forename: Ting
authors.initials: T
authors.lastname: Hu
authors.forename: Zi-Yi
authors.initials: ZY
authors.lastname: Li
authors.forename: Zhuo-Lie
authors.initials: ZL
authors.lastname: Huang
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D001685:Biological Factors / Q000737:chemistry* / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D010084:Oxidation-Reduction; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D001685:Biological Factors; D011134:Polysaccharides
keywords: Antioxidant activity; Free radical scavenging activity; Pleurotus tuber-regium (Fr.) Sing.; Polysaccharides