title: Construction of a highly stable artificial glutathione peroxidase on a protein nanoring.
abstract: Stable Protein One (SP1) is a boiling-stable oligomeric protein. The unique characteristics of SP1 offer a scaffold to design artificial enzymes against extreme temperature. Here, an efficient antioxidase is successfully constructed on the ring-shaped SP1 homododecamer. By means of computational design and genetic engineering, the active center of glutathione peroxidase (GPx), selenocysteine (Sec), is introduced to the SP1 monomer surface, and the self-assembly properties of the protein monomer lead to a ring-shaped SP1 with homododecamer catalytic selenium centers. This artificial selenoenzyme exhibits high GPx catalytic activity and shows a typical ping-pong kinetic mechanism. Moreover, it has a significantly broader temperature range and high thermostability. Owing to having multi-GPx active centers on a SP1 oligomer, this selenium-containing biomacromolecule exerts an excellent capability to protect cells from oxidative damage at the mitochondrial level. This strategy represents a new way to develop thermostable artificial nanoenzymes for some specific applications.
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, China. junqiuliu@jlu.edu.cn luoquan@jlu.edu.cn.
authors.forename: Lu
authors.initials: L
authors.lastname: Miao
authors.forename: Xiyu
authors.initials: X
authors.lastname: Zhang
authors.forename: Chengye
authors.initials: C
authors.lastname: Si
authors.forename: Yuzhou
authors.initials: Y
authors.lastname: Gao
authors.forename: Linlu
authors.initials: L
authors.lastname: Zhao
authors.forename: Chunxi
authors.initials: C
authors.lastname: Hou
authors.forename: Oded
authors.initials: O
authors.lastname: Shoseyov
authors.forename: Quan
authors.initials: Q
authors.lastname: Luo
authors.forename: Junqiu
authors.initials: J
authors.lastname: Liu
mesh_terms: D055162:Biocatalysis; D005818:Genetic Engineering; D005979:Glutathione Peroxidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D049329:Nanostructures / Q000737:chemistry*; D055550:Protein Stability; D011789:Quantum Theory; D017279:Selenocysteine / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013696:Temperature
chemical_list: D017279:Selenocysteine; D005979:Glutathione Peroxidase