title: Reproduction of the beet cyst nematode Heterodera schachtii Schm. on transformed root cultures of Beta vulgaris L.
abstract: Transformed hairy root cultures of Beta vulgaris L., grown in petri-dishes, were inoculated with a suspension of surface-sterilized larvae of the beet cyst nematode Heterodera schachtii Schm. Larvae developed into both male and female adults. Juvenile larvae were hatched from the newly-formed cysts, indicating that fertilization had occurred. Results of a glasshouse test showed that H. schachtii did not lose its pathogenicity after being cultured on transformed roots. This technique can be developed further for the mass-propagation of sedentary nematodes and for the in vitro storage of isclates.
authors.affiliation: Foundation for Agricultural Plant Breeding, P.O. Box 117, NL-6700 AC, Wageningen, The Netherlands.
authors.forename: H
authors.initials: H
authors.lastname: Paul
authors.forename: C
authors.initials: C
authors.lastname: Zijlstra
authors.forename: J E
authors.initials: JE
authors.lastname: Leeuwangh
authors.forename: F A
authors.initials: FA
authors.lastname: Krens
authors.forename: H J
authors.initials: HJ
authors.lastname: Huizing