title: Specific rare cell capture using micro-patterned silicon nanowire platform.
abstract: We report on the rapid and direct quantification of specific cell captures using a micro-patterned streptavidin (STR)-functionalized silicon nanowire (SiNW) platform, which was prepared by Ag-assisted wet chemical etching and a photo-lithography process. This platform operates by high-affinity cell capture rendered by the combination of antibody-epithelial cell surface-binding, biotin-streptavidin binding, and the topologically enhanced cell-substrate interaction on a 3-dimensional SiNWs array. In this work, we developed a micro-patterned nanowire platform, with which we were able to directly evaluate the performance enhancement due to nanotopography. An excellent capture efficiency of ~96.6±6.7%, which is the highest value achieved thus far for the targeting specific A549 cells on a selective area of patterned SiNWs, is demonstrated. Direct comparison between the nanowire region and the planar region on the same substrate indicates dramatically elevated cell-capture efficiency on nanotopological surface identical surface chemistry (<2% cell-capture efficiency). An excellent linear response was seen for quantifying captured A549 cells with respect to loaded cells. This study suggests that the micro-patterned STR-functionalized SiNWs platform provides additional advantage for detecting rare cells populations in a more quantitative and specific manner.
authors.affiliation: Department of Physics, Chung-Ang University, Seoul 156-756, Republic of Korea. Electronic address: sangkwonlee@cau.ac.kr.
authors.forename: Sang-Kwon
authors.initials: SK
authors.lastname: Lee
authors.affiliation: Basic Research Laboratory (BRL), Department of Semiconductor Science and Technology, Chonbuk National University, Jeonju 561-756, Republic of Korea.
authors.forename: Dong-Joo
authors.initials: DJ
authors.lastname: Kim
authors.affiliation: Department of Physics, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: GeeHee
authors.initials: G
authors.lastname: Lee
authors.affiliation: Basic Research Laboratory (BRL), Department of Semiconductor Science and Technology, Chonbuk National University, Jeonju 561-756, Republic of Korea.
authors.forename: Gil-Sung
authors.initials: GS
authors.lastname: Kim
authors.affiliation: Department of Biomedical Engineering, Yale University, New Haven, CT 06511, USA.
authors.forename: Minsuk
authors.initials: M
authors.lastname: Kwak
authors.affiliation: Department of Biomedical Engineering, Yale University, New Haven, CT 06511, USA; Yale Comprehensive Cancer Center, New Haven, CT 06520, USA.
authors.forename: Rong
authors.initials: R
authors.lastname: Fan
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D001710:Biotin / Q000378:metabolism; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000295:instrumentation*; D006801:Humans; D053770:Nanowires / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000175:diagnosis*; D011485:Protein Binding; D012825:Silicon / Q000737:chemistry*; D019809:Streptavidin / Q000378:metabolism; D013499:Surface Properties; D046888:Tissue Array Analysis
chemical_list: D001710:Biotin; D019809:Streptavidin; D012825:Silicon
keywords: Biocompatibility; Cell capture; Circulating tumor cells (CTCs); Filopodia; Silicon nanowires; Streptavidin functionalization