title: Glutamate dehydrogenase of the unicellular green alga Scenedesmus acutus : Substrate-induced conformational transition.
abstract: The coenzyme-non-specific glutamate dehydrogenase (EC 1.4.1.3) from Scenedesmus acutus in inhibited by p-hydroxymercuribenzoate only in the deamination reaction. From this result and from its stability in the presence of urea it is concluded that this enzyme exhibits and equilibrium between three conformations: aminating and deaminating conformations induced by NADH-2-oxoglutarate and NAD(+)-glutamate, respectively, and the "native" conformation in the absence of substrates.
authors.affiliation: Fakultät für Biologie der Universität, Postfach 5560, D-7750, Konstanz, Germany.
authors.forename: V R
authors.initials: VR
authors.lastname: Shatilov
authors.forename: H
authors.initials: H
authors.lastname: Sund