title: Alkaloids from Toddalia asiatica and their cytotoxic, antimicrobial and antifungal activities.
abstract: Phytochemical investigation of the ethanol extract from the roots of Toddalia asiatica resulted in the isolation of eight new alkaloids, 8-methoxynorchelerythrine (1), 11-demethylrhoifoline B (2), 8-methoxynitidine (3), 8-acetylnorchelerythrine (4), 8,9,10,12-tetramethoxynorchelerythrine (5), isointegriamide (6), 1-demethyl dicentrinone (7), and 11-hydroxy-10-methoxy-(2,3)-methylenedioxytetrahydroprotoberberine (8), together with 10 known alkaloids. Their structures were determined on the basis of spectroscopic analyses, including 1D-NMR, 2D-NMR, and HR-ESI-MS. The isolated components were evaluated in vitro for cytotoxic activities against eight tumor cell lines, antimicrobial activities against two Gram-positive bacteria and five Gram-negative bacteria, and antifungal activities against five pathogens. Benzo[c]phenanthridine and secobenzo[c]phenantridine alkaloids exhibited significant cytotoxic, antimicrobial and antifungal properties.
authors.affiliation: College of Biological Resources and Environment Science, Qujing Normal University, Qujing 655011, China; Institute of Characteristic Medicinal Resource of Ethnic Minorities, Qujing Normal University, Qujing 655011, China. Electronic address: hujiang@ustc.edu.
authors.forename: Jiang
authors.initials: J
authors.lastname: Hu
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Shi
authors.forename: Jiangang
authors.initials: J
authors.lastname: Chen
authors.forename: Xia
authors.initials: X
authors.lastname: Mao
authors.forename: Lei
authors.initials: L
authors.lastname: Zhu
authors.forename: Long
authors.initials: L
authors.lastname: Yu
authors.forename: Junyou
authors.initials: J
authors.lastname: Shi
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003603:Cytotoxins / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D027821:Rutaceae / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D003603:Cytotoxins; D010936:Plant Extracts
keywords: Alkaloids; Antifungal; Antimicrobial; Cytotoxic; Rutaceae; Toddalia asiatica