title: Influence of low hemoglobin in children with iron deficiency anemia on PFA-100 testing.
abstract: BACKGROUND
  Iron deficiency anemia may cause platelet aggregation dysfunction. The present study evaluated alterations of primary hemostasis in children with iron deficiency anemia caused by low hemoglobin using the PFA-100 instrument.
  
  
  METHODS
  This study analyzed 65 children between the ages of 2 and 14 with iron deficiency anemia (39 male and 26 female). The diagnosis criteria of IDA involved hemoglobin values below 11 g/dL, hematocrit values below 33%, erythrocyte counts below 3.7 x 10(12)/L, MCV values below 70 fL. Mean corpuscular hemoglobin (MCH) values below 27 pg, and ferritin levels below 12 ng/mL. Children without bleeding symptoms with blood hemoglobin values > 11g/dL and hematocrit values above 33%, defined in our previous study, were enrolled as the control group for the PFA-100 closure time. The ranges for prolonged C/EPI and C/ADP closure times in our center were > 160 seconds and > 110 seconds, respectively.
  
  
  RESULTS
  There was no significant correlation between hemoglobin values and C/EPI nor with C/ADP values.
  
  
  CONCLUSIONS
  This study found that low hemoglobin values do not play a role in obtaining C/EPI values above 160 seconds and C/ADP values above 110 seconds. We concluded that, patients with low hemoglobin values have no effect on the results of PFA-100, which is the screening test used to diagnose specific disorders such as von Willebrand disease or platelet secretion disorders.
authors.affiliation: Denizli State Hospital, Department of Pediatric Hematology, Denizli, Turkey. drmehmetakin@yahoo.com.tr
authors.forename: Mehmet
authors.initials: M
authors.lastname: Akin
mesh_terms: D000293:Adolescent; D018798:Anemia, Iron-Deficiency / Q000097:blood* / Q000503:physiopathology; D002648:Child; D002675:Child, Preschool; D004906:Erythrocyte Count; D005260:Female; D005293:Ferritins / Q000097:blood; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis*; D006487:Hemostasis; D006801:Humans; D008297:Male
chemical_list: D006454:Hemoglobins; D005293:Ferritins