title: [miR-124 suppresses cell proliferation and invasion in gastric carcinoma and its mechanism].
abstract: OBJECTIVE
  To explore the molecular mechanism of miR-124 suppressing the proliferation and invasion of gastric cancer cells.
  
  
  METHODS
  SPHK1 3'UTR-luciferase vector was constructed and luciferase reporter gene assay was employed to examine the effect of miR-124 on luciferase activity. Human gastric cancer MGC-803 cells were transfected with miR-124 mimics, and then Western blot was performed to detect the expression of SPHK1 protein.
  
  
  RESULTS
  Luciferase reporter vector system confirmed that SPHK1 was a target gene of miR-124. Western blot showed that the expression of SPHK1 protein was inhibited by miR-124. After transfection of miR-124 mimics or SPHK1 siRNA for 12 h, 24 h and 48 h, respectively, MTT assay showed that the A values of the three groups were significantly different (P < 0.05), and it was in a time-dependent manner. After transfection of miR-124 mimics or SPHK1 siRNA for 24 h, transwell invasion assay showed that the number of transmembrane cells was 54.6 ± 8.3 in the SPHK1 siRNA group and 47.8 ± 6.6 in the miR-124 mimics group, both were significantly lower than 100.6 ± 11.3 of the control group (P < 0.05), indicating that SPHK1 siRNA can slow down the invasion of MGC-803 cells.
  
  
  CONCLUSIONS
  miR-124 can suppress the cell proliferation and invasion by targeting SPHK1 in gastric carcinoma.
authors.affiliation: Department of Medical Oncology, the First Affiliated Hospital of University of South China, Hengyang 421001, China.
authors.forename: Li-ming
authors.initials: LM
authors.lastname: Xie
authors.forename: Rong-fang
authors.initials: RF
authors.lastname: He
authors.forename: Zhi-wei
authors.initials: ZW
authors.lastname: Zhang
authors.forename: Yun-yun
authors.initials: YY
authors.lastname: Tang
authors.forename: Zhao-yang
authors.initials: ZY
authors.lastname: Luo
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005822:Genetic Vectors; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D014162:Transfection; D000099157:Sphingosine Kinase
chemical_list: D008156:Luciferases; D035683:MicroRNAs; D017853:Phosphotransferases (Alcohol Group Acceptor); D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D000099157:Sphingosine Kinase; C509207:MIRN124 microRNA, human