title: Preparation of carbon paste electrodes including poly(styrene) attached glycine-Pt(IV) for amperometric detection of glucose.
abstract: In this study, a novel carbon paste electrode that is sensitive to glucose was prepared using the nanoparticles modified (4-Formyl-3-methoxyphenoxymethyl) with polystyren (FMPS) with L-Glycine-Pt(IV) complexes. Polymeric nanoparticles having Pt(IV) ion were prepared from (4-Formyl-3-methoxyphenoxymethyl) polystyren, glycine and PtCl4 by template method. Glucose oxidase enzyme was immobilized to a modified carbon paste electrode (MCPE) by cross-linking with glutaraldehyde. Determination of glucose was carried out by oxidation of enzymatically produced H2O2 at 0.5 V vs. Ag/AgCl. Effects of pH and temperature were investigated, and optimum parameters were found to be 8.0 and 55°C, respectively. Linear working range of the electrode was 5.0×10(-6)-1.0×10(-3) M, R(2)=0.997. Storage stability and operational stability of the enzyme electrode were also studied. Glucose biosensor gave perfect reproducible results after 10 measurements with 2.3% relative standard deviation. Also, it had good storage stability (gave 53.57% of the initial amperometric response at the end of 33th day).
authors.affiliation: Department of Chemistry, Faculty of Sciences and Art, Nevşehir University, 50300 Nevşehir, Turkey.
authors.forename: Soner
authors.initials: S
authors.lastname: Dönmez
authors.affiliation: Department of Chemistry, Faculty of Sciences, Gazi University, 06500 Teknikokullar, Ankara, Turkey. Electronic address: fatma@gazi.edu.tr.
authors.forename: Fatma
authors.initials: F
authors.lastname: Arslan
authors.affiliation: Department of Chemistry, Faculty of Sciences, Gazi University, 06500 Teknikokullar, Ankara, Turkey.
authors.forename: Nurşen
authors.initials: N
authors.lastname: Sarı
authors.affiliation: Department of Chemistry, Faculty of Sciences, Gazi University, 06500 Teknikokullar, Ankara, Turkey; Department of Chemistry, Faculty of Sciences and Art, Kırklareli University, Kırklareli, Turkey.
authors.forename: Nurdan
authors.initials: N
authors.lastname: Kurnaz Yetim
authors.affiliation: Department of Chemistry, Faculty of Sciences, Gazi University, 06500 Teknikokullar, Ankara, Turkey.
authors.forename: Halit
authors.initials: H
authors.lastname: Arslan
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry; D005998:Glycine / Q000737:chemistry*; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry; D009944:Organoplatinum Compounds / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D009944:Organoplatinum Compounds; D011137:Polystyrenes; D002244:Carbon; D005949:Glucose Oxidase; D005947:Glucose; D005998:Glycine
keywords: Biosensor; Carbon paste; Glucose oxidase; Nanoparticule; Pt(IV) complexes