title: Expression of sclerostin scFv and the effect of sclerostin scFv on healing of osteoporotic femur fracture in rats.
abstract: Osteoporosis is a systemic metabolic disease characterized by low bone mass with deterioration of the bony microstructure which leads to both bone brittleness and increased risk of fracture. Sclerostin is a protein encoded by the SOST gene which is specifically expressed in osteocyte. Monoclonal antibodies of sclerostin can promote bone formation by antagonizing its inhibitory action. However, the effectiveness of monoclonal antibodies to exert such effects are limited by the large molecular mass and high immunogenicity. Here, we report that we purified a high immune affinity, single-chain antibody of SOST: SOST-single-chain Fv (scFv). Real-time polymerase chain reaction amplification of the variable regions of the heavy- and light-chain gene from a secretory anti-SOST antibody was performed. Animal experiments showed that SOST-scFv promoted bone healing in a rat model of osteoporosis.
authors.affiliation: Department of Orthopaedics, Beijing Shijitan Hospital Affiliated to Capital Medical University, Beijing, 100038, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Yao
authors.forename: Jie
authors.initials: J
authors.lastname: Ni
authors.forename: Yu
authors.initials: Y
authors.lastname: Hou
authors.forename: Lixiang
authors.initials: L
authors.lastname: Ding
authors.forename: Licheng
authors.initials: L
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000494:pharmacology / Q000627:therapeutic use*; D002118:Calcium / Q000097:blood; D002454:Cell Differentiation / Q000187:drug effects; D004195:Disease Models, Animal; D005260:Female; D005269:Femur / Q000293:injuries; D005819:Genetic Markers / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology; D015675:Osteocalcin / Q000097:blood; D010012:Osteogenesis / Q000187:drug effects; D058866:Osteoporotic Fractures / Q000473:pathology / Q000628:therapy*; D010710:Phosphates / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology / Q000627:therapeutic use; D057127:Single-Chain Antibodies / Q000378:metabolism / Q000502:physiology / Q000627:therapeutic use*; D014057:Tomography, X-Ray Computed
chemical_list: D019485:Bone Morphogenetic Proteins; D005819:Genetic Markers; D010710:Phosphates; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies; C486031:Sost protein, rat; D015675:Osteocalcin; D000469:Alkaline Phosphatase; D002118:Calcium