title: Isolation and culture of protoplasts from high anthocyanin-producing callus of sweet potato.
abstract: Optimum conditions for the isolation and culture of protoplasts from high anthocyanin-producing callus of sweet potato (Ipomoea batatas) were investigated. Growth phase of callus and the ratio of callus-enzyme solution affected the yield of protoplasts. Composition of the medium and protoplast density were examined for protoplast culture.Small colonies were regenerated from the protoplasts. Upon transfer to light a high amount of anthocyanin was accumulated in these colonies.
authors.affiliation: Department of Biology, Faculty of Science, Shinshu University, 390, Matsumoto, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nishimaki
authors.forename: M
authors.initials: M
authors.lastname: Nozue