title: Murine homologue of the human KIAA1199 is implicated in hyaluronan binding and depolymerization.
abstract: Recently, we have disclosed that human KIAA1199 (hKIAA1199) is a hyaluronan (HA) binding protein implicated in HA depolymerization. Although a murine homologue (mKiaa1199) was previously cloned, no information about the function of the molecule was available. Here, we show that cells transfected with mKiaa1199 cDNA selectively catabolized HA via the clathrin-coated pit pathway. A glycosaminoglycan-binding assay demonstrated the specific binding of mKiaa1199 to HA. These results were similar to our observations with hKIAA1199, although slight differences were found in the peak sizes of the minimum degradates of HA. We conclude that like hKIAA1199, mKiaa1199 is a hyaladherin, leading to HA depolymerization.
authors.affiliation: Innovative Beauty Science Laboratory, Kanebo Cosmetics Inc., 3-28, 5-chome, Kotobuki-cho, Odawara-shi, Kanagawa 250-0002, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yoshida
authors.forename: Aya
authors.initials: A
authors.lastname: Nagaoka
authors.forename: Sachiko
authors.initials: S
authors.lastname: Nakamura
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Sugiyama
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Okada
authors.forename: Shintaro
authors.initials: S
authors.lastname: Inoue
keywords: CHC, clathrin heavy chain; CPC, cetylpyridinium chloride; CS, chondroitin sulfate; Catabolism; DS, dermatan sulfate; Extracellular matrix; FA, fluoresceinamine; FBS, fetal bovine serum; GAG, glycosaminoglycan; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GlcNAc, N-acetylglucosamine; HA, hyaluronan; HEK, human embryonic kidney; HPLC, high performance liquid chromatography; HS, heparan sulfate; HYAL, hyaluronidase; Hep, heparin; Hyaluronan; KIAA1199; LDS, lithium dodecyl sulfate; ORF, open reading frame; PBS, phosphate-buffered saline; PBS-T, PBS containing 0.05% Tween 20; PCR, polymerase chain reaction; siRNA, short interfering RNA