title: Constitutive function of the Ikaros transcription factor in primary leukemia cells from pediatric newly diagnosed high-risk and relapsed B-precursor ALL patients.
abstract: We examined the constitutive function of the Ikaros (IK) transcription factor in blast cells from pediatric B-precursor acute lymphoblastic leukemia (BPL) patients using multiple assay platforms and bioinformatics tools. We found no evidence of diminished IK expression or function for primary cells from high-risk BPL patients including a Philadelphia chromosome (Ph)(+) subset. Relapse clones as well as very aggressive in vivo clonogenic leukemic B-cell precursors isolated from spleens of xenografted NOD/SCID mice that developed overt leukemia after inoculation with primary leukemic cells of patients with BPL invariably and abundantly expressed intact IK protein. These results demonstrate that a lost or diminished IK function is not a characteristic feature of leukemic cells in Ph(+) or Ph(-) high-risk BPL.
authors.affiliation: Systems Immunobiology Laboratory and Developmental Therapeutics Program, Children's Center for Cancer and Blood Diseases, Children's Hospital Los Angeles, Los Angeles, California, United States of America ; Department of Pediatrics, University of Southern California Keck School of Medicine, Los Angeles, California, United States of America ; Developmental Therapeutics Program, USC Norris Comprehensive Cancer Center, Los Angeles, California, United States of America.
authors.forename: Fatih M
authors.initials: FM
authors.lastname: Uckun
authors.forename: Hong
authors.initials: H
authors.lastname: Ma
authors.forename: Rita
authors.initials: R
authors.lastname: Ishkhanian
authors.forename: Martha
authors.initials: M
authors.lastname: Arellano
authors.forename: Anoush
authors.initials: A
authors.lastname: Shahidzadeh
authors.forename: Amanda
authors.initials: A
authors.lastname: Termuhlen
authors.forename: Paul S
authors.initials: PS
authors.lastname: Gaynon
authors.forename: Sanjive
authors.initials: S
authors.lastname: Qazi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002648:Child; D024202:Electrophoretic Mobility Shift Assay; D005091:Exons / Q000235:genetics; D005260:Female; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D052138:Genes, Neoplasm; D006801:Humans; D051740:Ikaros Transcription Factor / Q000235:genetics* / Q000378:metabolism; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D010677:Philadelphia Chromosome; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis* / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012008:Recurrence; D012307:Risk Factors; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: C497239:IKZF1 protein, human; D012333:RNA, Messenger; D051740:Ikaros Transcription Factor