title: Pathotyping bla CTX-M Escherichia coli from Nigeria.
abstract: BACKGROUND
  Escherichia coli have become the enterobacteriaceae species most affected by extended-spectrum β-lactamases (ESBLs) in view of the emergence of CTX-M-type ESBLs. These CTX-M-positive E. coli have been reported in numerous regions worldwide. Virulence determinants of already reported CTX-M-positive E. coli were investigated.
  
  
  METHODS
  To gain insights into the mechanism underlying this phenomenon, we assessed serogroup, susceptibility pattern and diversity of virulence profiles within a collection of nine bla CTX-M-positive E. coli strains and their virulent determinant using miniaturized DNA microarray techniques. The nine ESBL-positive E. coli isolates were from eight male and one female patient(s) selected for study based on previous work. Virulence potential was inferred by detection of 63 virulence factor (VF) genes.
  
  
  RESULTS
  Four (44.4%) of the 9 E. coli isolates exhibited the same set of core characteristics: serotype O8:Hnt, while all were positive for OXA-1, ciprofloxacin resistance. Five of the isolates exhibited highly similar (91% to 100%) VF profiles.
  
  
  CONCLUSIONS
  The findings describe a broadly disseminated, bla CTX-M-positive and virulent E. coli serogroup with highly homogeneous virulence genotypes, suggesting recent emergence in this zone. Understanding how this clone has emerged and successfully disseminated within the hospital and community, including across national boundaries, should be a public health priority.
authors.forename: Olugbenga Adekunle
authors.initials: OA
authors.lastname: Olowe
authors.forename: Suman
authors.initials: S
authors.lastname: Choudhary
authors.forename: Peter
authors.initials: P
authors.lastname: Schierack
authors.forename: Lothar H
authors.initials: LH
authors.lastname: Wieler
authors.forename: Olufunmilola Bamidele
authors.initials: OB
authors.lastname: Makanjuola
authors.forename: Albert B
authors.initials: AB
authors.lastname: Olayemi
authors.forename: Muna
authors.initials: M
authors.lastname: Anjum
keywords: blaCTX-M-positive, virulent E. coli, miniaturized DNA microarray, pathotyping, resistance