title: In vivo evaluation of safety and toxicity of a Lactobacillus jensenii producing modified cyanovirin-N in a rhesus macaque vaginal challenge model.
abstract: Sexual transmission of human immunodeficiency virus type 1 (HIV-1) across the cervicovaginal mucosa in women is influenced by many factors including the microbiota and the presence of underlying inflammation. It is important that potential HIV preventative agents do not alter the mucosal environment in a way that enhances HIV acquisition. We examined the impact of a "live" microbicide on the vaginal mucosal environment in a rhesus macaque repeated vaginal simian-HIV (SHIVSF162P3) challenge model. The microbicide contained a human vaginal Lactobacillus jensenii expressing the HIV-1 entry inhibitor, modified Cyanovirin-N (mCV-N), and henceforth called LB-mCV-N. Macaques were colonized vaginally each week with LB-mCV-N and sampled six days after colonization for culturable bacteria, pH and cervical-vaginal cytokines during the duration of the six-week study. We show that macaques that retained the engineered LB-mCV-N strain in their vaginal microbiota, during SHIV challenge, had lower pH, when colonization levels were higher, and had no evidence of inflammatory cytokines. Indeed, Interleukin-13, a mediator of inflammation, was detected less often in LB-mCV-N colonized macaques than in controls and we found higher levels of Interleukin 1 receptor antagonist (IL-1RA) in LB-mCV-N colonized macaques during the SHIV challenge period. We noted an inverse correlation between levels of mucosal IL-1RA and peak plasma viral load, thus higher IL-1RA correlated with lower viral load in LB-mCV-N treated macaques. These data support the use of LB-mCV-N as a safe "live" microbicide and suggest that lactobacilli themselves may positively impact the mucosal environment.
authors.affiliation: Vaccine Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Beda
authors.initials: B
authors.lastname: Brichacek
authors.forename: Laurel A
authors.initials: LA
authors.lastname: Lagenaur
authors.forename: Peter P
authors.initials: PP
authors.lastname: Lee
authors.forename: David
authors.initials: D
authors.lastname: Venzon
authors.forename: Dean H
authors.initials: DH
authors.lastname: Hamer
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local; D001426:Bacterial Proteins / Q000096:biosynthesis*; D015415:Biomarkers / Q000378:metabolism; D002352:Carrier Proteins / Q000096:biosynthesis*; D060085:Coinfection; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004198:Disease Susceptibility / Q000276:immunology; D005260:Female; D006863:Hydrogen-Ion Concentration; D018836:Inflammation Mediators / Q000378:metabolism; D007778:Lactobacillus / Q000378:metabolism*; D008253:Macaca mulatta; D008597:Menstrual Cycle; D064307:Microbiota; D009092:Mucous Membrane / Q000378:metabolism / Q000382:microbiology / Q000821:virology; D012307:Risk Factors; D016097:Simian Acquired Immunodeficiency Syndrome / Q000382:microbiology / Q000517:prevention & control / Q000635:transmission / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000276:immunology; D014621:Vagina / Q000382:microbiology* / Q000821:virology; D014627:Vaginitis / Q000276:immunology / Q000378:metabolism / Q000382:microbiology / Q000821:virology
chemical_list: D000891:Anti-Infective Agents, Local; D001426:Bacterial Proteins; D015415:Biomarkers; D002352:Carrier Proteins; D016207:Cytokines; D018836:Inflammation Mediators; C107733:cyanovirin N