title: Calcitonin gene-related peptide immunoreactive sensory neurons in the vagal and glossopharyngeal ganglia innervating the larynx of the rat.
abstract: We have examined whether calcitonin gene-related peptide-immunoreactive (CGRP-ir) neurons in the vagal and glossopharyngeal ganglia innervate the larynx. Many CGRP-ir neurons were located mostly in the superior glossopharyngeal-jugular ganglion complex that was fused the superior glossopharyngeal ganglion and the jugular ganglion in the cranial cavity. When Fluorogold was applied to the cut end of the superior laryngeal nerve (SLN) or the recurrent laryngeal nerve (RLN), many Fluorogold-labeled neurons were found in the superior glossopharyngeal-jugular ganglion complex and the nodose ganglion. Double-labeling for CGRP and Fluorogold showed that about 80% of Fluorogold-labeled neurons in the superior glossopharyngeal-jugular ganglion complex expressed CGRP-like immunoreactivity in the case of application to the SLN, and about 50% of Fluorogold-labeled neurons expressed CGRP-like immunoreactivity in the case of the RLN. Only a few double-labeled neurons were found in the nodose ganglion. The number of the Fluorogold-labeled neurons and double-labeled neurons in the superior glossopharyngeal-jugular ganglion complex in the case of the SLN was larger than that in the case of the RLN. These results indicate that sensory information from the larynx might be conveyed by many CGRP-ir neurons located in the superior glossopharyngeal-jugular ganglion complex by way of the SLN and the RLN.
authors.affiliation: Department of Anatomy, Hyogo College of Medicine, Mukogawa, Nishinomiya, Hyogo 663-8501, Japan. Electronic address: tetsu@hyo-med.ac.jp.
authors.forename: Tetsu
authors.initials: T
authors.lastname: Hayakawa
authors.affiliation: Department of Anatomy, Hyogo College of Medicine, Mukogawa, Nishinomiya, Hyogo 663-8501, Japan.
authors.forename: Sachi
authors.initials: S
authors.lastname: Kuwahara-Otani
authors.affiliation: Department of Anatomy, Hyogo College of Medicine, Mukogawa, Nishinomiya, Hyogo 663-8501, Japan.
authors.forename: Seishi
authors.initials: S
authors.lastname: Maeda
authors.affiliation: Department of Anatomy, Hyogo College of Medicine, Mukogawa, Nishinomiya, Hyogo 663-8501, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: Department of Anatomy, Hyogo College of Medicine, Mukogawa, Nishinomiya, Hyogo 663-8501, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Seki
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D017950:Ganglia, Sensory / Q000378:metabolism*; D005930:Glossopharyngeal Nerve / Q000378:metabolism*; D007150:Immunohistochemistry; D007823:Laryngeal Nerves / Q000378:metabolism*; D007830:Larynx / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011984:Sensory Receptor Cells / Q000378:metabolism; D014630:Vagus Nerve / Q000378:metabolism*
chemical_list: D015740:Calcitonin Gene-Related Peptide
keywords: Cranial ganglia; Immunohistochemistry; Recurrent laryngeal nerve; Retrograde tracing; Superior laryngeal nerve