title: Side-chain amino-acid-based pH-responsive self-assembled block copolymers for drug delivery and gene transfer.
abstract: Developing safe and effective nanocarriers for multitype of delivery system is advantageous for several kinds of successful biomedicinal therapy with the same carrier. In the present study, we have designed amino acid biomolecules derived hybrid block copolymers which can act as a promising vehicle for both drug delivery and gene transfer. Two representative natural chiral amino acid-containing (l-phenylalanine and l-alanine) vinyl monomers were polymerized via reversible addition-fragmentation chain transfer (RAFT) process in the presence of monomethoxy poly(ethylene glycol) based macro-chain transfer agents (mPEGn-CTA) for the synthesis of well-defined side-chain amino-acid-based amphiphilic block copolymers, monomethoxy poly(ethylene glycol)-b-poly(Boc-amino acid methacryloyloxyethyl ester) (mPEGn-b-P(Boc-AA-EMA)). The self-assembled micellar aggregation of these amphiphilic block copolymers were studied by fluorescence spectroscopy, atomic force microscopy (AFM) and scanning electron microscopy (SEM). Potential applications of these hybrid polymers as drug carrier have been demonstrated in vitro by encapsulation of nile red dye or doxorubicin drug into the core of the micellar nanoaggregates. Deprotection of side-chain Boc- groups in the amphiphilic block copolymers subsequently transformed them into double hydrophilic pH-responsive cationic block copolymers having primary amino groups in the side-chain terminal. The DNA binding ability of these cationic block copolymers were further investigated by using agarose gel retardation assay and AFM. The in vitro cytotoxicity assay demonstrated their biocompatible nature and these polymers can serve as "smart" materials for promising bioapplications.
authors.affiliation: Polymer Research Centre, Department of Chemical Sciences, Indian Institute of Science Education and Research - Kolkata , BCKV Campus Main Office, Mohanpur 741252, Nadia, West Bengal, India.
authors.forename: Sonu
authors.initials: S
authors.lastname: Kumar
authors.forename: Rituparna
authors.initials: R
authors.lastname: Acharya
authors.forename: Urmi
authors.initials: U
authors.lastname: Chatterji
authors.forename: Priyadarsi
authors.initials: P
authors.lastname: De
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D016503:Drug Delivery Systems; D004952:Esters / Q000737:chemistry; D064347:Microscopy, Electrochemical, Scanning; D011108:Polymers / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D000596:Amino Acids; D004952:Esters; D011108:Polymers