title: HDAC4 does not act as a protein deacetylase in the postnatal murine brain in vivo.
abstract: Reversible protein acetylation provides a central mechanism for controlling gene expression and cellular signaling events. It is governed by the antagonistic commitment of two enzymes families: the histone acetyltransferases (HATs) and the histone deacetylases (HDACs). HDAC4, like its class IIa counterparts, is a potent transcriptional repressor through interactions with tissue specific transcription factors via its N-terminal domain. Whilst the lysine deacetylase activity of the class IIa HDACs is much less potent than that of the class I enzymes, HDAC4 has been reported to influence protein deacetylation through its interaction with HDAC3. To investigate the influence of HDAC4 on protein acetylation we employed the immunoaffinity-based AcetylScan proteomic method. We identified many proteins known to be modified by acetylation, but found that the absence of HDAC4 had no effect on the acetylation profile of the murine neonate brain. This is consistent with the biochemical data suggesting that HDAC4 may not function as a lysine deacetylase, but these in vivo data do not support the previous report showing that the enzymatic activity of HDAC3 might be modified by its interaction with HDAC4. To complement this work, we used Affymetrix arrays to investigate the effect of HDAC4 knock-out on the transcriptional profile of the postnatal murine brain. There was no effect on global transcription, consistent with the absence of a differential histone acetylation profile. Validation of the array data by Taq-man qPCR indicated that only protamine 1 and Igfbp6 mRNA levels were increased by more than one-fold and only Calml4 was decreased. The lack of a major effect on the transcriptional profile is consistent with the cytoplasmic location of HDAC4 in the P3 murine brain.
authors.affiliation: Department of Medical and Molecular Genetics, King's College London, London, United Kingdom.
authors.forename: Michal
authors.initials: M
authors.lastname: Mielcarek
authors.forename: Tamara
authors.initials: T
authors.lastname: Seredenina
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Stokes
authors.forename: Georgina F
authors.initials: GF
authors.lastname: Osborne
authors.forename: Christian
authors.initials: C
authors.lastname: Landles
authors.forename: Linda
authors.initials: L
authors.lastname: Inuabasi
authors.forename: Sophie A
authors.initials: SA
authors.lastname: Franklin
authors.forename: Jeffrey C
authors.initials: JC
authors.lastname: Silva
authors.forename: Ruth
authors.initials: R
authors.lastname: Luthi-Carter
authors.forename: Vahri
authors.initials: V
authors.lastname: Beaumont
authors.forename: Gillian P
authors.initials: GP
authors.lastname: Bates
mesh_terms: D000107:Acetylation; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000201:enzymology*; D003593:Cytoplasm / Q000201:enzymology; D015536:Down-Regulation / Q000235:genetics; D020869:Gene Expression Profiling; D006655:Histone Deacetylases / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D020411:Oligonucleotide Array Sequence Analysis; D021381:Protein Transport; D015203:Reproducibility of Results; D014158:Transcription, Genetic; D015854:Up-Regulation / Q000235:genetics
chemical_list: C498618:Hdac5 protein, mouse; D006655:Histone Deacetylases