title: Phytochemical evaluation of Lythrum salicaria extracts and their effects on guinea-pig ileum.
abstract: n-Hexane, chloroform, ethyl acetate and 50% ethanol in water extracts prepared from the air-dried flowering parts of Lythrum salicaria L. were tested for in vitro pharmacological properties on Guinea-pig ileum, which is suitable for detecting a whole range of neuronal and smooth muscle effects. UHPLC-MS was used to evaluate polyphenol components of the extracts. In the ileum, the most prominent response (46.4% related to 0.5 microM histamine) of the extracts causing smooth muscle contractions were triggered by the 50% ethanol in water extract in a concentration-dependent manner. Atropine, indomethacin and PPADS plus suramin significantly reduced the contractile response caused by this extract. The strongest inhibition was due to atropine. The results suggest that L. salicaria extracts have a moderate muscarinic receptor agonist effect in Guinea-pig ileum and that prostanoids and purinoceptor mechanisms are involved to some extent. Therefore diluted extracts of L. salicaria p.o. could be used as a mild stimulant of gastrointestinal motility. The 50% ethanol in water extract was rich in polyphenols. n-Hexane, chloroform and ethyl acetate extracts failed to contain catechin, caffeic acid, quercetin-3-D-galactoside and rutin, but they all showed spasmogenic effects, and, therefore we do not think that these compounds could be involved in the spasmogenic activity.
authors.affiliation: Department of Pharmacognosy, University of Pécs, Rókus st. 2., H-7624 Pécs, Hungary. timea.bencsik@aok.pte.hu
authors.forename: Tímea
authors.initials: T
authors.lastname: Bencsik
authors.forename: Loránd
authors.initials: L
authors.lastname: Barthó
authors.forename: Viktor
authors.initials: V
authors.lastname: Sándor
authors.forename: Nóra
authors.initials: N
authors.lastname: Papp
authors.forename: Rita
authors.initials: R
authors.lastname: Benkó
authors.forename: Attila
authors.initials: A
authors.lastname: Felinger
authors.forename: Ferenc
authors.initials: F
authors.lastname: Kilár
authors.forename: Györgyi
authors.initials: G
authors.lastname: Horváth
mesh_terms: D000818:Animals; D005260:Female; D005765:Gastrointestinal Agents / Q000032:analysis*; D005769:Gastrointestinal Motility / Q000187:drug effects*; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects*; D031561:Lythrum / Q000737:chemistry*; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry
chemical_list: D005765:Gastrointestinal Agents; D010936:Plant Extracts