title: Isolation and identification of bacteria capable of degrading euptox A from Eupatorium adenophorum Spreng.
abstract: Eupatorium adenophorum Spreng (E. adenophorum), an invasive plant which has caused widespread poisoning of livestock and crop failures in China. 9-oxo-10, 11-dehydroageraphorone (known as euptox A) is the main toxin causing a series of respiratory and digestive system diseases in animals. The purpose of the research is to isolate and identify the bacteria with the capacity of degrading euptox A, in which way could we lay the foundation in the utilization and prevention for E. adenophorum. Three strains of bacteria with the degradative characteristic are screened from E. adenophorum leaves, soil and goat's rumen respectively by nutrition enrichment. The 16S rDNA sequence of the three isolated strains (XC-07, XC-08, XC-09) were shown to be 97%, 100%, 100% similar to Stenotrophmonas sp., Klebsiella sp. and Pseudomonas sp., respectively, named as Stenotrophomonas sp. XC-07, Klebsiella sp. XC-08 and Pseudomonas sp. XC-09. The degradation rates of 3 strains to euptox A (45 mg/L) in 24 h are 91.2%, 94.3% and 93.2%, respectively, which are determined by HPLC.
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Yaan 625014, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liao
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Yaan 625014, China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Yaan 625014, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Yaan 625014, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Mo
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Yaan 625014, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Tan
authors.affiliation: College of Life Science, Northwest University, Xian 710069, China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Yaan 625014, China. Electronic address: hychun114@163.com.
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Hu
mesh_terms: D036603:Ageratina / Q000737:chemistry*; D000818:Animals; D001483:Base Sequence; D002681:China; D006041:Goats; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D058865:Introduced Species; D007709:Klebsiella; D008969:Molecular Sequence Data; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry*; D011549:Pseudomonas; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012417:Rumen / Q000737:chemistry*; D017422:Sequence Analysis, DNA; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D012987:Soil / Q000737:chemistry*; D020588:Stenotrophomonas
chemical_list: C098165:9-oxo-10,11-dehydroagerophorone; D012336:RNA, Ribosomal, 16S; D012717:Sesquiterpenes; D012987:Soil
keywords: Eupatorium adenophorum Spreng; Euptox A; Microbiological degradation