title: Flow sorting and culture of protoplasts: Conditions for high-frequency recovery, growth and morphogenesis from sorted protoplasts of suspension cultures of nicotiana.
abstract: Protoplasts were prepared from suspension cultures of Nicotiana tabacum cv Wisconsin 38 that had been prelabeled with FITC. The protoplasts were subjected to flow sorting based on fluorescence content using a Coulter EPICS V Flow Cytometer - Cell Sorter. Conditions were established that allowed the recovery after sorting of approximately 30% of the initial protoplasts in a viable state. These were subsequently regenerated into calli that underwent shoot morphogenesis.
authors.affiliation: School of Biological Sciences, University of Nebraska-Lincoln, 68588-0118, Lincoln, Nebraska, USA.
authors.forename: D W
authors.initials: DW
authors.lastname: Galbraith
authors.forename: C L
authors.initials: CL
authors.lastname: Afonso
authors.forename: K R
authors.initials: KR
authors.lastname: Harkins