title: Complement-activating IgM enhances the humoral but not the T cell immune response in mice.
abstract: IgM antibodies specific for a certain antigen can enhance antibody responses when administered together with this antigen, a process believed to require complement activation by IgM. However, recent data show that a knock-in mouse strain, Cμ13, which only produces IgM unable to activate complement, has normal antibody responses. Moreover, the recently discovered murine IgM Fc receptor (FcµR or TOSO/FAIM3) was shown to affect antibody responses. This prompted the re-investigation of whether complement activation by specific IgM is indeed required for enhancement of antibody responses and whether the mutation in Cµ13 IgM also caused impaired binding to FcµR. The results show that IgM from Cµ13 and wildtype mice bound equally well to the murine FcµR. In spite of this, specific Cμ13 IgM administered together with sheep red blood cells or keyhole limpet hemocyanine was a very poor enhancer of the antibody and germinal center responses as compared with wildtype IgM. Within seconds after immunization, wildtype IgM induced deposition of C3 on sheep red blood cells in the blood. IgM which efficiently enhanced the T-dependent humoral immune response had no effect on activation of specific CD4(+) T cells as measured by cell numbers, cell division, blast transformation, or expression of the activation markers LFA-1 and CD44 in vivo. These observations confirm the importance of complement for the ability of specific IgM to enhance antibody responses and suggest that there is a divergence between the regulation of T- and B-cell responses by IgM.
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
authors.forename: Zhoujie
authors.initials: Z
authors.lastname: Ding
authors.forename: Anna
authors.initials: A
authors.lastname: Bergman
authors.forename: Christian
authors.initials: C
authors.lastname: Rutemark
authors.forename: Rika
authors.initials: R
authors.lastname: Ouchida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohno
authors.forename: Ji-Yang
authors.initials: JY
authors.lastname: Wang
authors.forename: Birgitta
authors.initials: B
authors.lastname: Heyman
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism; D003167:Complement Activation / Q000276:immunology*; D003165:Complement System Proteins / Q000276:immunology; D055879:Gene Knock-In Techniques; D056724:Immunity, Humoral; D007075:Immunoglobulin M / Q000276:immunology* / Q000378:metabolism; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism; D051379:Mice; D011961:Receptors, Fc / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; C499656:Fcmr protein, mouse; D007075:Immunoglobulin M; D008565:Membrane Proteins; D011961:Receptors, Fc; C035311:immunoglobulin M receptor; D003165:Complement System Proteins