title: Isolation and quantitation of isopentenyladenosine in an anise cell culture by single-ion monitoring, radioimmunoassay and bioassay.
abstract: Using three different techniques, isopentenyladenosine was identified and quantitated in an anise cell line (Pimpinella anisum L.), growing on a medium without cytokinin. A method to quantitate cytokinins was developed which utilizes extraction of cytokinins in the presence of a deuterated reference compound and subsequent quantitation of the cytokinins by single-ion monitoring on a gas chromatography-mass spectrometry system. The results were compared with those obtained by radioimmunoassay as well as a bioassay. Quantitations by gas chromatography-mass spectrometry and radioimmunoassay correlated well, whereas the estimates by the bioassay gave considerably lower values.
authors.affiliation: Max-Planck-Institut für Biochemie, D-8033, Martinsried, Federal Republic of Germany.
authors.forename: D
authors.initials: D
authors.lastname: Ernst
authors.forename: W
authors.initials: W
authors.lastname: Schäfer
authors.forename: D
authors.initials: D
authors.lastname: Oesterhelt