title: MiRNA-218, a new regulator of HMGB1, suppresses cell migration and invasion in non-small cell lung cancer.
abstract: MicroRNAs (miRNAs) function as negative regulators of gene expression involved in cancer metastasis. The aim of this study is to investigate the potential roles of miR-218 in non-small cell lung cancer and validate its regulation mechanism. Functional studies showed that miR-218 overexpression inhibited cell migration and invasion, but had no effect on cell viability. Enhanced green fluorescent protein reporter assay, real-time polymerase chain reaction and western blot analysis confirmed that miR-218 suppressed the expression of high mobility group box-1 (HMGB1) by directly targeting its 3'-untranslated region. Accordingly, silencing of HMGB1 accorded with the effects of miR-218 on cell migration and invasion, and overexpression of HMGB1 can restore cell migration and invasion which were reduced by miR-218. In conclusion, these findings demonstrate that miR-218 functions as a tumor suppressor in lung cancer. Furthermore, miR-218 may act as a potential therapeutic biomarker for metastatic lung cancer patients.
authors.affiliation: Department of Geriatrics, The Affiliated Hospital of Yan'an University, Yan'an 716000, China.
authors.forename: Cailian
authors.initials: C
authors.lastname: Zhang
authors.forename: Shengli
authors.initials: S
authors.lastname: Ge
authors.forename: Cailian
authors.initials: C
authors.lastname: Hu
authors.forename: Ning
authors.initials: N
authors.lastname: Yang
authors.forename: Jinran
authors.initials: J
authors.lastname: Zhang
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D024243:HMGB1 Protein / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D020413:3' Untranslated Regions; D024243:HMGB1 Protein; C547471:MIRN218 microRNA, human; D035683:MicroRNAs; D049452:Green Fluorescent Proteins
keywords: HMGB1; miR-218; microRNA; non-small lung cancer