title: Influence of the titania nanotubes dimensions on adsorption of collagen: an experimental and computational study.
abstract: To investigate the influence of the titanium nanotube (TiNT) diameters on the adsorption of collagen type I (Col-I), TiNTs with different diameters were prepared with anodization. The adsorption amount of Col-I on the different TiNTs substrates was evaluated by spectrophotometric measurement and immunofluorescence staining, respectively. The results showed that the diameters of TiNTs played a key role in the adsorption process of Col-I. TiNTs with diameters around 100nm displayed a higher adsorption amount and faster adsorption speed than that of 30nm TiNTs. Furthermore, more collagen molecules were aggregated in the tubes of 100nm TiNTs. Molecular dynamics simulation was performed to elucidate the adsorption mechanism. The simulation results confirmed that physical adsorption was the main driving force, including van der Waals force and hydrogen bond between Col-I molecules and TiNTs. The calculated interaction energies indicated that the TiNTs with bigger dimensions had higher interaction energies, thus leading to the higher collagen adsorption.
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China.
authors.forename: Weihu
authors.initials: W
authors.lastname: Yang
authors.forename: Xingfeng
authors.initials: X
authors.lastname: Xi
authors.forename: Qichun
authors.initials: Q
authors.lastname: Ran
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Kaiyong
authors.initials: K
authors.lastname: Cai
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D000818:Animals; D003094:Collagen / Q000737:chemistry*; D004566:Electrodes; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D017433:Protein Structure, Secondary; D051381:Rats; D055672:Static Electricity; D013816:Thermodynamics; D014025:Titanium / Q000737:chemistry*
chemical_list: C009495:titanium dioxide; D003094:Collagen; D014025:Titanium
keywords: Collagen type I; Molecular dynamics simulation; Protein adsorption; Titania nanotubes