title: Proteomics in investigation of cancer metastasis: functional and clinical consequences and methodological challenges.
abstract: Metastases are responsible for most of the cases of death in patients with solid tumors. There is thus an urgent clinical need of better understanding the exact molecular mechanisms and finding novel therapeutics targets and biomarkers of metastatic disease of various tumors. Metastases are formed in a complicated biological process called metastatic cascade. Up to now, proteomics has enabled the identification of number of metastasis-associated proteins and potential biomarkers in cancer tissues, microdissected cells, model systems, and secretomes. Expression profiles and biological role of key proteins were confirmed in verification and functional experiments. This communication reviews these observations and analyses the methodological aspects of the proteomics approaches used. Moreover, it reviews contribution of current proteomics in the field of functional characterization and interactome analysis of proteins involved in various events in metastatic cascade. It is evident that ongoing technical progress will further increase proteome coverage and sample capacity of proteomics technologies, giving complex answers to clinical and functional questions asked.
authors.affiliation: Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.
authors.forename: Josef
authors.initials: J
authors.lastname: Maryáš
authors.forename: Jakub
authors.initials: J
authors.lastname: Faktor
authors.forename: Monika
authors.initials: M
authors.lastname: Dvořáková
authors.forename: Iva
authors.initials: I
authors.lastname: Struhárová
authors.forename: Peter
authors.initials: P
authors.lastname: Grell
authors.forename: Pavel
authors.initials: P
authors.lastname: Bouchal
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D042282:Microdissection; D009362:Neoplasm Metastasis / Q000235:genetics* / Q000473:pathology; D009369:Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D040901:Proteomics
chemical_list: D014408:Biomarkers, Tumor
keywords: Biomarkers; Biomedicine; Functional studies; Metastasis; Surface proteins