title: The preparation of gypenosides liposomes and its effects on the peritoneal macrophages function in vitro.
abstract: Optimization of preparation of gypenosides liposome (GPSL) was investigated with response surface methodology (RSM) and the effect of GPSL activating phagocytosis activity and cytokine secretion of macrophages was determined in the paper. The results showed that the optimal conditions of GPSL preparation were as follows: the ratio of lipid to drug (w/w) of 8:1, the ratio of soybean phospholipid to cholesterol (w/w) of 6:1, the temperature of incubation of drug in water bath of 55°C, the time of incubation of drug of 11 min. With the condition, the experimental encapsulation efficiency of GPSL was 90.17 ± 0.38%. In addition, GPSL could significantly enhance phagocytosis activity and the secretion of cytokines of the peritoneal macrophages of mice. These indicated that GPSL with high entrapping efficiency and small particle size could be prepared by ethanol injection combined with ammonium sulfate gradient method, which is applied easily, and GPSL could enhance the activity of peritoneal macrophages.
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Yu
authors.affiliation: National Research Center of Veterinary Biologicals Engineering and Technology, Jiangsu Academy of Agricultural Science, Nanjing 210014, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Ruonan
authors.initials: R
authors.lastname: Bo
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yee
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jiaguo
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Tao
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: dywang@njau.edu.cn.
authors.forename: Deyun
authors.initials: D
authors.lastname: Wang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002626:Chemistry, Pharmaceutical; D002784:Cholesterol / Q000737:chemistry; D016207:Cytokines / Q000378:metabolism; D029774:Gynostemma / Q000737:chemistry; D008081:Liposomes; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D010316:Particle Size; D010587:Phagocytosis / Q000187:drug effects; D010743:Phospholipids / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry
chemical_list: D016207:Cytokines; D008081:Liposomes; D010743:Phospholipids; D010936:Plant Extracts; C474918:gypenoside; D002784:Cholesterol
keywords: Cytokine; Gypenosides liposome; Optimization; Peritoneal macrophages; Phagocytic activity; Response surface methodology