title: Structure of glycoproteins from Acacia gum: an assembly of ring-like glycoproteins modules.
abstract: The glycoprotein (GP) molecular fraction structure of the gum exudate of Acacia senegal (gum Arabic) isolated from hydrophobic interaction chromatography was investigated using high-performance size exclusion chromatography-multi angle laser light scattering (HPSEC-MALLS), small angle X-ray scattering (SAXS), synchrotron radiation circular dichroism (SRCD) and transmission electron microscopy (TEM) observations. In solution, GP would be a mixture of spheroidal monomers and more anisotropic oligomers as suggested by the two exponent values found in the Rg vs. Mw relationship and TEM observations. The GP conformation probed by SAXS was ascribed to a thin object with a triaxial ellipsoid morphology, certainly attributed to GP oligomers. A 9 nm diameter particle was also identified by SAXS in agreement with the dimensions identified by TEM on single isolated ring-like structures. The GP oligomerization process, as probed by TEM, would be the result of ring-like subunits self-association. This self-association would lead to more linear or, sometimes, cyclised assembly. At the molecular level, GP fraction was found to have secondary structures mainly made of β-sheets and turns (64%) but also, to a lesser extent, made of polyproline II (PPII) and α-helices (19%). These features were characteristic of hydroxyprolin-rich glycoproteins with arabinosylated and arabinogalactan polysaccharide side chains grafted to the polypeptide backbone. The GP molecular fraction structure from Acacia gum would be an assembly of ring-like glycoproteins modules. These ring-like structures were certainly due to hydroxyproline (Hyp)-arabinogalactan (AG) subunits.
authors.affiliation: INRA, UR1268 Biopolymères Interactions Assemblages, F-44300 Nantes, France. Electronic address: drenard@nantes.inra.fr.
authors.forename: D
authors.initials: D
authors.lastname: Renard
authors.forename: E
authors.initials: E
authors.lastname: Lepvrier
authors.forename: C
authors.initials: C
authors.lastname: Garnier
authors.forename: P
authors.initials: P
authors.lastname: Roblin
authors.forename: M
authors.initials: M
authors.lastname: Nigen
authors.forename: C
authors.initials: C
authors.lastname: Sanchez
mesh_terms: D002850:Chromatography, Gel; D005685:Galactans / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry* / Q000648:ultrastructure; D006170:Gum Arabic / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D006909:Hydroxyproline / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D008970:Molecular Weight; D017433:Protein Structure, Secondary; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction
chemical_list: D005685:Galactans; D006023:Glycoproteins; D006170:Gum Arabic; D006909:Hydroxyproline; C005653:arabinogalactan
keywords: Acacia gum; Glycoprotein; HPSEC–MALLS; Small-angle X-ray scattering; Synchrotron radiation circular dichroism; Transmission electron microscopy