title: Changes in DNA composition in the evolution of Vicia species.
abstract: The composition of nuclear DNA in 3 Vicia species are compared. The species V. eriocarpa, V. johannis and V. melanops are from three separate subgeneric sections of Vicia and show a fourfold variation in their amounts of nuclear DNA. DNA melting experiments, buoyant density gradient analysis and Cot reassociation experiments show that the quantitiative change in nuclear DNA between the three species is achieved by changes in the amounts of both repetitive and nonrepetitive DNA sequences. It is suggested that while the increase in the repetitive fraction is achieved by the proliferation of repetitive base sequences the increase in the nonrepetitive fraction is due to the steady accretion of highly diverged base sequences resulting from mutations, deletions, insertions and base sequence rearrangements among families of repetitive sequences.
authors.affiliation: Department of Agricultural Botany, University College of Wales, Penglais, SY23 3DD, Aberystwyth, Dyfed, Wales.
authors.forename: S N
authors.initials: SN
authors.lastname: Raina
authors.forename: R K
authors.initials: RK
authors.lastname: Narayan