title: Isolation of an auxotrophic cell line of soybean (Glycine max) which requires asparagine or glutamine for growth.
abstract: A suspension culture of haploid soybean cells was treated with ultra-violet light. From the mutagenized culture an auxotrophic cell line was isolated which grows on 1-B5 media supplemented with asparagine or glutamine. In the absence of these additives the cells cease to grow and die. Asparagine is unable to serve as a sole source of nitrogen, i.e., cannot substitute for nitrate and ammonia.
authors.affiliation: Department of Biology, The University of Utah, 84112, Salt Lake City, UT, USA.
authors.forename: E J
authors.initials: EJ
authors.lastname: Roth
authors.forename: K G
authors.initials: KG
authors.lastname: Lark