title: cDNA cloning of β-tubulin gene and organization of tubulin genes in Vigna radiata (mung bean) genome.
abstract: We isolated an almost full-length cDNA clone containing β-tubulin gene from a partial cDNA library of mung bean using chicken cDNA as probe. Cross-hybridization with chicken β-tubulin cDNA and positive hybridization-selection and translation of mung bean mRNA established that this clone contains β-tubulin sequences. We studied the organization of tubulin genes in mung bean. In this plant tubulin genes are organized in tandem repeats of alternating α- and β-tubulin genes. The 5.6 kb basis repeat unit which contains both α- and β-tubulin genes is repeated twenty times per haploid genome.
authors.affiliation: Department of Biochemistry, Bose Institute, Centenary Building, P-1/12, CIT Scheme VII M, 700 054, Calcutta, India.
authors.forename: D
authors.initials: D
authors.lastname: Raha
authors.forename: K
authors.initials: K
authors.lastname: Sen
authors.forename: B B
authors.initials: BB
authors.lastname: Biswas