title: Somatic embryogenesis and plant regeneration from leaf tissues of Panicum maximum Jacq.
abstract: Somatic embryogenesis was induced in proliferating leaf segments of Panicum maximum Jacq., cultured on Murashige and Skoog's nutrient medium containing 2,4-dichlorophenoxyacetic acid and coconut milk. The embryoids gave rise to plants on a medium containing gibberellic acid. The plants were successfully transplanted to soil and grown to maturity. Histological examination of proliferating leaves showed that the embryogenic callus tissue was formed by divisions in cells of the lower epidermis as well as the mesophyll tissue. The regenerated plants showed the normal chromosome number of 2n=4x=32.
authors.affiliation: Department of Botany, University of Florida, Gainesville, USA.
authors.forename: C
authors.initials: C
authors.lastname: Lu
authors.forename: I K
authors.initials: IK
authors.lastname: Vasil