title: Vortex-assisted liquid-liquid micro-extraction and high-performance liquid chromatography for a higher sensitivity methyl methacrylate determination in biological matrices.
abstract: A vortex-assisted liquid-liquid micro-extraction coupled with high-performance liquid chromatography, with UV-vis, is proposed to pre-concentrate methyl methacrylate and to improve separation in biological matrices. The use of 1-octanol as extracting phase, its volume, the need for a dispersant agent, the agitation conditions and the cooling time before phase separation were evaluated. In optimum conditions, enrichment factors of 20 (±0.5) and enrichment recovery of 99% were obtained. The straightforward association of this extraction process with the HPLC method, previously regulated by the International Organization for Standardization, afforded a detection limit of 122 ng/mL and a quantification limit of 370 ng/mL. The within-batch precision, relative standard deviation, was 3% for a sample with 1.49 µg/mL and 4% for a sample with 13.4 µg/mL. The results showed a between batch-precision of 21% for experiments performed on five different days, for a sample with a concentration of 1.10 µg/mL in methyl methacrylate.
authors.affiliation: REQUIMTE/Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, R. Jorge Viterbo Ferreira 228, 4050-313, Porto, Portugal.
authors.forename: Tiago F A
authors.initials: TF
authors.lastname: Sousa
authors.forename: Marta C
authors.initials: MC
authors.lastname: Aniceto
authors.forename: Célia G
authors.initials: CG
authors.lastname: Amorim
authors.forename: Mariana
authors.initials: M
authors.lastname: Souto-Lopes
authors.forename: Daniel
authors.initials: D
authors.lastname: Pérez-Mongiovi
authors.forename: Maria C B S M
authors.initials: MC
authors.lastname: Montenegro
authors.forename: Alberto N
authors.initials: AN
authors.lastname: Araújo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D059627:Liquid Phase Microextraction / Q000379:methods*; D020366:Methylmethacrylate / Q000032:analysis* / Q000302:isolation & purification*; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D020366:Methylmethacrylate
keywords: high-performance liquid chromatography; methyl methacrylate; saliva samples; vortex-assisted liquid-liquid micro-extraction