title: Synthesis and cardio protective biological applications of glucodendrimers by H9C2 cell studies.
abstract: Novel glucodendrimers scaffolds containing α-d-glucopyranoside at the surface and triazole as bridging unit have been synthesized. Cardiomyocytes were exposed to normal and high glucose level in the presence and absence of glucodendrimers. Cytotoxicity studies were also carried out and the expression of metalloproteinases mainly MMP-2 and 9 was confirmed with gelatine zymography and RT-PCR gene expression studies. Cardio protective efficiency of the synthesized glucodendrimers against high glucose induced toxicity on mettalloproteinase-2 and 9 and also on H9C2 cell lines revealed that higher generation glucodendrimers 6 and 8 are more effective than the lower generation glucodendrimers.
authors.affiliation: Department of Organic Chemistry, University of Madras, Maraimalai Campus, Chennai 600025, Tamil Nadu, India. Electronic address: perumalrajakumar@gmail.com.
authors.forename: Perumal
authors.initials: P
authors.lastname: Rajakumar
authors.forename: Ramasamy
authors.initials: R
authors.lastname: Anandhan
authors.forename: Gangadhara Prasadachari
authors.initials: GP
authors.lastname: Vadla
authors.forename: Elangovan
authors.initials: E
authors.lastname: Vellaichamy
mesh_terms: D000818:Animals; D002316:Cardiotonic Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D050091:Dendrimers / Q000138:chemical synthesis / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D005947:Glucose / Q000037:antagonists & inhibitors / Q000494:pharmacology; D005960:Glucosides / Q000737:chemistry; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D013329:Structure-Activity Relationship; D014230:Triazoles / Q000737:chemistry
chemical_list: D002316:Cardiotonic Agents; D050091:Dendrimers; D005960:Glucosides; D014230:Triazoles; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D005947:Glucose
keywords: Click chemistry; Diabetic mellitus; Glucodendrimer; H9C2 cardiomyocytes; Matrix metalloproteinases