title: BolOST1, an ortholog of Open Stomata 1 with alternative splicing products in Brassica oleracea, positively modulates drought responses in plants.
abstract: Open Stomata 1 (OST1), an ABA-activated sucrose non-fermenting 1 (SNF1)-related protein kinase, is critical for plant drought responses. We investigated the functions of two splicing isoforms of the OST1 ortholog in Brassica oleracea (BolOST1). BolOST1 expression was found to be dramatically induced by drought and high-salt stress, and the ectopic expression of BolOST1 restored the drought-sensitive phenotype of ost1. Subcellular localization revealed that BolOST1 is localized in both the nucleus and cytoplasm. BolOST1 was also demonstrated to phosphorylate the N-terminal fragment of ABI5 (ABA Insensitive 5, ABI5-N). A firefly luciferase complementation assay revealed that BolOST1 interacts with both BolABI5 and an ABI1 ortholog in B. oleracea (BolABI1). Overall, these results suggest that BolOST1 is a functional SnRK2-type protein kinase and that the early ABA signaling network may be conserved between Arabidopsis and cabbage.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Mengyao
authors.initials: M
authors.lastname: Wang
authors.forename: Feifei
authors.initials: F
authors.lastname: Yuan
authors.forename: Hongmei
authors.initials: H
authors.lastname: Hao
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Huixian
authors.initials: H
authors.lastname: Zhao
authors.forename: Aiguang
authors.initials: A
authors.lastname: Guo
authors.forename: Jingjiang
authors.initials: J
authors.lastname: Hu
authors.forename: Xiaona
authors.initials: X
authors.lastname: Zhou
authors.forename: Chang Gen
authors.initials: CG
authors.lastname: Xie
mesh_terms: D017398:Alternative Splicing; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000502:physiology*; D001937:Brassica / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D055864:Droughts; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology*; D011494:Protein Kinases / Q000235:genetics / Q000502:physiology*; D013312:Stress, Physiological / Q000235:genetics / Q000502:physiology*
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D010940:Plant Proteins; D011494:Protein Kinases
keywords: ABI5; BolABI1; BolABI5; BolOST1; Drought responses; OST1