title: Enzymic synthesis of o-succinylbenzoyl-CoA in cell-free extracts of anthraquinone producing Galium mollugo L. cell suspension cultures.
abstract: o-Succinylbenzoic acid (OSB) is an intermediate in the biosynthesis of shikimatederived anthraquinones. The cell free activation of o-succinylbenzoic acid in extracts of anthraquinone producing cells of Galium mollugo L. is demonstrated for the first time. This activation depends on the presence of ATP, coenzyme A and Mg(2+). The o-succinylbenzoic acid coenzyme A ester was identified by converting it to 1,4-dihydroxy-2-naphthoic acid by a bacterial enzyme, viz. naphthoatesynthase. It is thus demonstrated that the o-succinylbenzoic acid coenzyme A ester derived from bacteria and from Galium mollugo cells are identical.
authors.affiliation: Institut für Pharmazeutische Biologie und Phytochemie der Westfälischen Wilhelms-Universität, Hittorfstraße 56, D-4400, Münster, F.R.G..
authors.forename: L
authors.initials: L
authors.lastname: Heide
authors.forename: R
authors.initials: R
authors.lastname: Kolkmann
authors.forename: S
authors.initials: S
authors.lastname: Arendt
authors.forename: E
authors.initials: E
authors.lastname: Leistner