title: Triplex-mediated analysis of cytosine methylation at CpA sites in DNA.
abstract: Modified triplex-forming oligonucleotides distinguish 5-methyl cytosine from unmethylated cytosine in DNA duplexes by differences in triplex melting temperatures. The discrimination is sequence-specific; dramatic differences in stabilisation are seen for CpA methylation, whereas CpG methylation is not detected. This direct detection of DNA methylation constitutes a new approach for epigenetic analysis.
authors.affiliation: School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK. tom.brown@chem.ox.ac.uk.
authors.forename: Marie W
authors.initials: MW
authors.lastname: Johannsen
authors.forename: Simon R
authors.initials: SR
authors.lastname: Gerrard
authors.forename: Tracy
authors.initials: T
authors.lastname: Melvin
authors.forename: Tom
authors.initials: T
authors.lastname: Brown
mesh_terms: D044503:5-Methylcytosine / Q000032:analysis*; D018899:CpG Islands; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D019175:DNA Methylation; D006863:Hydrogen-Ion Concentration; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D044366:Transition Temperature
chemical_list: D009841:Oligonucleotides; D044503:5-Methylcytosine; D004247:DNA