title: Structure and function of the peanut panallergen Ara h 8.
abstract: The incidence of peanut allergy continues to rise in the United States and Europe. Whereas exposure to the major allergens Ara h 1, 2, 3, and 6 can cause fatal anaphylaxis, exposure to the minor allergens usually does not. Ara h 8 is a minor allergen. Importantly, it is the minor food allergens that are thought to be responsible for oral allergy syndrome (OAS), in which sensitization to airborne allergens causes a Type 2 allergic reaction to ingested foods. Furthermore, it is believed that similar protein structure rather than a similar linear sequence is the cause of OAS. Bet v 1 from birch pollen is a common sensitizing agent, and OAS results when patients consume certain fruits, vegetables, tree nuts, and peanuts. Here, we report the three-dimensional structure of Ara h 8, a Bet v 1 homolog. The overall fold is very similar to that of Bet v 1, Api g 1 (celery), Gly m 4 (soy), and Pru av 1 (cherry). Ara h 8 binds the isoflavones quercetin and apigenin as well as resveratrol avidly.
authors.affiliation: From the Southern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, New Orleans, Louisiana 70124.
authors.forename: Barry K
authors.initials: BK
authors.lastname: Hurlburt
authors.forename: Lesa R
authors.initials: LR
authors.lastname: Offermann
authors.forename: Jane K
authors.initials: JK
authors.lastname: McBride
authors.forename: Karolina A
authors.initials: KA
authors.lastname: Majorek
authors.forename: Soheila J
authors.initials: SJ
authors.lastname: Maleki
authors.forename: Maksymilian
authors.initials: M
authors.lastname: Chruszcz
mesh_terms: D000485:Allergens / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D052179:Antigens, Plant / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D000077102:Apium / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D010367:Arachis; D029662:Betula / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D005512:Food Hypersensitivity / Q000235:genetics / Q000276:immunology; D006801:Humans; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011794:Quercetin / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D040681:Structural Homology, Protein
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D010940:Plant Proteins; D011794:Quercetin
keywords: Allergen; Antigen; Crystal Structure; Flavonoids; Fluorescence; Peanut