title: Anti-inflammatory activity of ethanolic extract of Sargassum micracanthum.
abstract: The anti-inflammatory effects of Sargassum micracanthum ethanol extract (SMEE) was investigated using LPS-induced inflammatory response in this study. As a result, there was no cytotoxicity in the macrophage proliferation treated with SMEE compared with the control. SMEE inhibited production of nitric oxide and cytokines (IL-6, TNF-α, and IL-1β) in a dose-dependent manner. In addition, the expression of inducible nitric oxide synthase and cyclooxygenase 2 were suppressed via inhibition of nuclear factor κB p65 expression by SMEE treatment. The formation of edema in the mouse ear was reduced at the highest dose tested compared with that in the control, and reduction of ear thickness was observed in histological analysis. Moreover, in an acute toxicity test, no mortalities occurred in mice administered 5,000 mg/kg body weight of SMEE over a 2-week observation period. These results suggest that SMEE may have significant effects on inflammatory mediators and be a potential antiinflammatory therapeutic material.
authors.affiliation: Department of Food Science and Technology/Institute of Food Science, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: Da-Hyun
authors.initials: DH
authors.lastname: Jeong
authors.forename: Koth-Bong-Woo-Ri
authors.initials: KB
authors.lastname: Kim
authors.forename: Min-Ji
authors.initials: MJ
authors.lastname: Kim
authors.forename: Bo-Kyeong
authors.initials: BK
authors.lastname: Kang
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Ahn
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004423:Ear / Q000473:pathology; D004487:Edema / Q000139:chemically induced; D007249:Inflammation / Q000517:prevention & control; D008070:Lipopolysaccharides / Q000276:immunology* / Q000633:toxicity*; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D044647:Sargassum / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D008070:Lipopolysaccharides; D016328:NF-kappa B; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D051546:Cyclooxygenase 2