title: Overexpression of a new stress-repressive gene OsDSR2 encoding a protein with a DUF966 domain increases salt and simulated drought stress sensitivities and reduces ABA sensitivity in rice.
abstract: Domain of Unknown Function 966 (DUF966) gene family was found in the protein family database, which consisted of seven genes in rice. The proteins encoded by these genes contained one or two highly conserved DUF966 domains. The available data of public microarray databases implied that these genes might play crucial roles in plant response to abiotic stresses. In this study, a member of the DUF966 gene family, DUF966-stress repressive gene 2 in Oryza sativa (OsDSR2, Loc_Os01g62200), was cloned and its role in rice responding to salt and simulated drought stresses was functionally characterized. OsDSR2 was expressed mainly in nodes of stems and leaf blades from rice. Expression profile analysis of adversity showed that OsDSR2 had different transcriptional responses to salt, drought, cold, heat and oxidative (H2O2) stresses, as well as abscisic acid (ABA), methyl jasmonate, salicylic acid, gibberellin acid and auxin treatments. Transient expression demonstrated that OsDSR2 was localized in the membrane and nucleus. Overexpression of OsDSR2 could increase salt and simulated drought (polyethyleneglycol)-stress sensitivities in rice by downregulating the expression of ABA- and stress-responsive genes including OsNCED4, SNAC1, OsbZIP23, P5CS, Oslea3 and rab16C. Furthermore, OsDSR2-overexpressing plants showed reduced ABA sensitivity during the post-germination stage. These results suggested that OsDSR2 negatively regulated rice response to salt and simulated drought stresses as well as ABA signaling, which provided some useful data for understanding the functional roles of DUF966 family genes in abiotic stress responses in plants.
authors.affiliation: Xiamen Key Laboratory for Plant Genetics, School of Life Sciences, Xiamen University, Xiamen, 361005, China.
authors.forename: Chengke
authors.initials: C
authors.lastname: Luo
authors.forename: Chiming
authors.initials: C
authors.lastname: Guo
authors.forename: Wujing
authors.initials: W
authors.lastname: Wang
authors.forename: Liangjiang
authors.initials: L
authors.lastname: Wang
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D019295:Computational Biology; D055864:Droughts; D035264:Flowers / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D015870:Gene Expression; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D018525:Germination; D009928:Organ Specificity; D012275:Oryza / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D017434:Protein Structure, Tertiary; D036226:Seedlings / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins; D012965:Sodium Chloride; D000040:Abscisic Acid