title: Karyotyping and in situ chromosomal localization of rDNA sites in black cumin Bunium persicum (Boiss) B. Fedtsch,1915 (Apiaceae).
abstract: The fluorescent in situ hybridization (FISH) technique has been applied to somatic chromosomes in the medicinally important species, Bunium persicum, to elucidate its karyotypes. The bicolour FISH technique involving 18S-5.8S-26S and 5S ribosomal RNA genes as probes was used to assign physical localization and measurement of rDNA sites on homologous pairs of chromosomes. The two 18S-5.8S-26S rRNA gene sites were at the terminal regions of the short arms of the chromosomes 1 and 2 involving NOR region of chromosome 1. The 5S rDNA sites were found on subtelomeric region of the long arm of the chromosome number 5 and at interstitial regions of the short arm of chromosome 7. Based on direct visual analysis of chromosome length, morphology and position of FISH signals, a pioneer attempt has been made to construct metaphase karyotype in Bunium persicum, an endangered medicinal plant of North Western Himalayas.
authors.affiliation: Department of Agricultural Biotechnology, CSK Himachal Pradesh Agricultural University, Palampur- 176 062 (HP) India.
authors.forename: R K
authors.initials: RK
authors.lastname: Chahota
authors.forename: Y
authors.initials: Y
authors.lastname: Mukai
authors.forename: H K
authors.initials: HK
authors.lastname: Chaudhary
authors.forename: Naval
authors.initials: N
authors.lastname: Kishore
authors.forename: T R
authors.initials: TR
authors.lastname: Sharma
keywords: Bunium persicum; FISH; black cumin; karyotypes