title: [Isolation and expression profiling of transformer 2 gene in Aedes aegypti].
abstract: OBJECTIVE
  To isolate, identify and analyze the sex-determining gene Transformer 2 (Aaetra2) of the major vector mosquito Aedes aegypti.
  
  
  METHODS
  tBLASTn program, RT-PCR and RACE methods were used to obtain the full-length cDNA of Aaetra2. Multiple alignments of nucleotide and amino acid sequences were conducted, and the different domains in tra2 protein were indentified. RT-PCR of the total RNA extracted from different tissue from the mosquitoes in different developmental stages was performed using specific primers.
  
  
  RESULTS
  Two genes, namely Aaetra2-α and Aaetra2-β, were identified in different supercontig locations. The multi-transcripts were expressed by means of alternative promoters or terminators. The different domains in tra2 protein were defined as RS-rich N-terminal region, RNA recognition motif-RRM, linker region, and RS-rich C-terminal region. Both Aaetra2-α and Aaetra2-β showed sustained expression throughout the developmental stages of Ae.aegypti, and in all the tissues without a sex specificity.
  
  
  CONCLUSIONS
  Aaetra2 gene has multiple isoforms and is mapped to multiple locations in the genome. Aaetra2 has conservative functional domains of the sex-determining gene tra2. For Ae.agypti, Aaetra2 shows the potential as a new target for release of insects carrying a dominant lethal (RIDL) technology based on transgenic mosquitoes.
authors.affiliation: Key Laboratory of Prevention and Control for Emerging Infectious Diseases of Guangdong Higher Institutes, Department of Pathogen Biology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.E-mail: 15625045004@163.com.
authors.forename: Peiwen
authors.initials: P
authors.lastname: Liu
authors.forename: Yuting
authors.initials: Y
authors.lastname: Chen
authors.forename: Jinbao
authors.initials: J
authors.lastname: Gu
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Chen
mesh_terms: D000330:Aedes / Q000235:genetics* / Q000254:growth & development; D000595:Amino Acid Sequence; D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics* / Q000302:isolation & purification; D009419:Nerve Tissue Proteins / Q000235:genetics; D010802:Phylogeny; D016601:RNA-Binding Proteins / Q000235:genetics; D012261:Ribonucleoproteins / Q000235:genetics; D016415:Sequence Alignment; D000068103:Serine-Arginine Splicing Factors; D012733:Sex Differentiation / Q000235:genetics*
chemical_list: D029721:Drosophila Proteins; D019476:Insect Proteins; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C067777:Sxl protein, Drosophila; C097083:TRA2B protein, human; C066174:tra2 protein, Drosophila; D000068103:Serine-Arginine Splicing Factors