title: Incorporation of haem into phycocyanobilin in levulinic acid treated Cyanidium caldarium.
abstract: Cells of the red alga Cyanidium caldarium were preincubated with 5 mmol 1(-1) levulinic acid (LA) and subsequently incubated with (14)C-labelled haem (5.67 Bq nmol(-1)). Phycocyanin was isolated. The specific radio-activity of its chromophore phycocyanobilin (PCB) was determined after cleavage and purification by thin layer chromatography. The percentage of PCB formed from labelled haem within 0.5 h was considerably higher in LA treated cells than in non-treated controls. This difference disappeared after prolonged incubation (16.5 h) with haem. The results are interpreted as possible incorporation of haem into preexisting apoprotein.
authors.affiliation: Botanisches Institut der Universität München, Menzinger Straße 67, D-8000, München 19, FRG.
authors.forename: A
authors.initials: A
authors.lastname: Schuster
authors.forename: H P
authors.initials: HP
authors.lastname: Köst
authors.forename: W
authors.initials: W
authors.lastname: Rüdiger
authors.forename: J A
authors.initials: JA
authors.lastname: Holroyd
authors.forename: S B
authors.initials: SB
authors.lastname: Brown