title: Larvicidal Activity of Centaurea bruguierana ssp. belangerana Against Anopheles stephensi Larvae.
abstract: In this study, the total 80% of MeOH extract and also petroleum ether, CHCl3, EtOAc, n-BuOH, and the remaining MeOH fractions obtained by solvent-solvent fractionation of the whole flowering samples of Centaurea bruguierana (DC.) Hand.-Mzt. ssp. belangerana (DC.) Bornm. (Asteraceae), namely "Baad-Avard", collected from Borazjan in Bushehr Province (Bushehr, Iran) were investigated for larvicidal activity against malaria vector, Anopheles stephensi Liston, according to WHO methods. The mortality rate of total extract and petroleum ether fraction in concentration of 40 ppm were 28% and 86% respectively and the other fractions were inactive. The probit regression analysis for the dose-response to petroleum ether fraction treatment of larvae exhibited the LC50 and LC90 values of 15.7 ppm and 48.3 ppm, respectively. As results showed, the larvicidal activity of the petroleum ether fraction would be due to the nonpolar compounds in the plant which further isolation and purification would obtain the more active compounds in lower concentrations useful for preparation of biological insecticides.
authors.affiliation: Department of Pharmacognosy and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, 14155-6451, Iran.
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Khanavi
authors.forename: Afsaneh
authors.initials: A
authors.lastname: Rajabi
authors.forename: Masoud
authors.initials: M
authors.lastname: Behzad
authors.forename: Abbas
authors.initials: A
authors.lastname: Hadjiakhoondi
authors.forename: Hassan
authors.initials: H
authors.lastname: Vatandoost
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Abaee
keywords: Anopheles stephensi larvae; Asteraceae; Centaurea bruguierana ssp. Belangerana; Larvicidal activity