title: Impact of harmonization of collection tubes on Alzheimer's disease diagnosis.
abstract: OBJECTIVE
  The objective of this study was to analyze differences in biomarker outcomes before and after harmonization of cerebrospinal fluid (CSF) collection tubes in Alzheimer's disease (AD) diagnosis.
  
  
  METHODS
  We analyzed data from French memory centers that switched from different CSF collection tubes to a common one. A total of 1966 patients were included in the study. CSF concentrations of β-amyloid 1-42 (Aβ42), total tau, and phosphorylated tau (p-tau181) were measured in each center using the same commercial enzyme-linked immunoabsorbent assay (ELISA) kits. The diagnostic value of CSF biomarkers according to the type of tube used was then assessed using different cutoffs.
  
  
  RESULTS
  The predictive value of Aβ42 was highly affected by the type of collection tube used. The optimal cutoff value for p-tau181 appeared not to be affected by the type of collection tube whereas that of total tau was slightly changed. New optimal cutoff values were then computed.
  
  
  CONCLUSIONS
  In a routine clinical environment, the selection of the collection tube and biomarker cutoff value makes a major difference in AD biological diagnosis. The use of a common collection tube among different centers will reduce the risk of misdiagnosis and incorrect patient stratification.
authors.affiliation: CHU de Montpellier, IRB, INSERM-UM1 1040, Montpellier, France. Electronic address: s-lehmann@chu-montpellier.fr.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Lehmann
authors.affiliation: Université Lille Nord de France, INSERM U837, Lille University Hospital, Center of Biology and Pathology, Lille, France.
authors.forename: Susanna
authors.initials: S
authors.lastname: Schraen
authors.affiliation: Service de Neurobiologie, Hospices Civils de Lyon, Université Lyon 1-CNRS UMR5292-INSERM U1028, Lyon, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Quadrio
authors.affiliation: CMRR Paris Nord Ile de France, Lariboisiere-Fernand Widal Hospital, APHP, University 7-Denis Diderot, Paris, France; INSERM U942, Paris, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Paquet
authors.affiliation: Université Lille Nord de France, EA1046, DISTALZ, Memory Center, CHU 59000, Lille, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Bombois
authors.affiliation: CHU de Montpellier, IRB, INSERM-UM1 1040, Montpellier, France; Paris 7, Faculté de Médecine Xavier Bichat, Paris, France.
authors.forename: Constance
authors.initials: C
authors.lastname: Delaby
authors.affiliation: CMRR Lyon, Hôpital des Charpennes, HCL Lyon, Lyon, France.
authors.forename: Aline
authors.initials: A
authors.lastname: Dorey
authors.affiliation: CMRR Paris Nord Ile de France, Lariboisiere-Fernand Widal Hospital, APHP, University 7-Denis Diderot, Paris, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Dumurgier
authors.affiliation: CHU de Montpellier, IRB, INSERM-UM1 1040, Montpellier, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Hirtz
authors.affiliation: CMRR Lyon, Hôpital des Charpennes, HCL Lyon, Lyon, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Krolak-Salmon
authors.affiliation: Laboratoire de Biochimie, Lariboisière-Fernand Widal Hospital, APHP, University Paris 7-Denis Diderot, University Paris Descartes, Paris, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Laplanche
authors.affiliation: CMRR, CHU de Grenoble, Grenoble, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Moreaud
authors.affiliation: Laboratoire de Biochimie, Lariboisière-Fernand Widal Hospital, APHP, University Paris 7-Denis Diderot, University Paris Descartes, Paris, France.
authors.forename: Katell
authors.initials: K
authors.lastname: Peoc'h
authors.affiliation: CMRR, CHU Dijon, Dijon, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Rouaud
authors.affiliation: Université Lille Nord de France, INSERM U837, Lille University Hospital, Center of Biology and Pathology, Lille, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Sablonnière
authors.affiliation: CMRR, CHU de Montpellier, Montpellier, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Thouvenot
authors.affiliation: CMRR, CHU de Montpellier, Montpellier, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Touchon
authors.affiliation: Université Lille Nord de France, EA1046, DISTALZ, Memory Center, CHU 59000, Lille, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Vercruysse
authors.affiliation: CMRR Paris Nord Ile de France, Lariboisiere-Fernand Widal Hospital, APHP, University 7-Denis Diderot, Paris, France; INSERM U942, Paris, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Hugon
authors.affiliation: CMRR, CHU de Montpellier, Montpellier, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Gabelle
authors.affiliation: Université Lille Nord de France, EA1046, DISTALZ, Memory Center, CHU 59000, Lille, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Pasquier
authors.affiliation: Service de Neurobiologie, Hospices Civils de Lyon, Université Lyon 1-CNRS UMR5292-INSERM U1028, Lyon, France.
authors.forename: Armand
authors.initials: A
authors.lastname: Perret-Liaudet
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid*; D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid*; D015415:Biomarkers / Q000134:cerebrospinal fluid; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005602:France; D006801:Humans; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000134:cerebrospinal fluid*; D010766:Phosphorylation; D016875:tau Proteins / Q000134:cerebrospinal fluid*
chemical_list: D016229:Amyloid beta-Peptides; D015415:Biomarkers; C054369:MAPT protein, human; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D016875:tau Proteins
keywords: Alzheimer's disease; Biomarkers; Cerebrospinal fluid; Diagnosis; Preanalytics