title: A new strategy for recovery of two peptides without Glu employing glutamate-specific endopeptidase from Bacillus licheniformis.
abstract: The difficulty in the purification of bioactive peptide limited its application in food, drug and cosmetic industry. Here we report a new strategy for the recovery of two peptides employing glutamate-specific endopeptidase from Bacillus licheniformis (GSE-BL), which shows strong specificity for Glu residue. Human glucagon and human beta-defensin-2 (HBD-2) were peptides without Glu residue, and Glu residue was introduced between affinity tag and target peptide as recognition site of GSE-BL. Tagless human glucagon with the same HPLC retention time as native human glucagon and mature HBD-2 with antibacterial activity and cytotoxicity were obtained after GSE-BL treatment. This strategy has great potential in the recovery of bioactive peptide without Glu residue, thus facilitating large scale preparation of peptide and widening the application of bioactive peptide.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China; State Key Laboratory of Applied Microbiology, South China (The Ministry-Province Joint Development), Guangdong Institute of Microbiology, GuangZhou 510070, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ye
authors.forename: Yi
authors.initials: Y
authors.lastname: Ma
authors.forename: Haiying
authors.initials: H
authors.lastname: Wang
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Luo
authors.forename: Weimin
authors.initials: W
authors.lastname: Zhang
authors.forename: Jufang
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Wang
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D001709:Biotechnology; D002851:Chromatography, High Pressure Liquid; D010450:Endopeptidases / Q000378:metabolism*; D005934:Glucagon / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D018698:Glutamic Acid / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity; D023083:beta-Defensins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
chemical_list: C106641:DEFB4A protein, human; D010455:Peptides; D011994:Recombinant Proteins; D023083:beta-Defensins; D018698:Glutamic Acid; D005934:Glucagon; D010450:Endopeptidases
keywords: GSE-BL; HBD; His-Tag; Human beta defensin-2; Human glucagon; glutamate-specific endopeptidase from Bacillus licheniformis; human beta defensin