title: A probable risk factor of female breast cancer: study on benign and malignant breast tissue samples.
abstract: The study reports enhanced Fe, Cu, and Zn contents in breast tissues, a probable risk factor of breast cancer in females. Forty-one formalin-fixed breast tissues were analyzed using atomic absorption spectrophotometry. Twenty malignant, six adjacent to malignant and 15 benign tissues samples were investigated. The malignant tissues samples were of grade 11 and type invasive ductal carcinoma. The quantitative comparison between the elemental levels measured in the two types of specimen (benign and malignant) tissues (removed after surgery) suggests significant elevation of these metals (Fe, Cu, and Zn) in the malignant tissue. The specimens were collected just after mastectomy of women aged 19 to 59 years from the hospitals of Islamabad and Rawalpindi, Pakistan. Most of the patients belong to urban areas of Pakistan. Findings of study depict that these elements have a promising role in the initiation and development of carcinoma as consistent pattern of elevation for Fe, Cu, and Zn was observed. The results showed the excessive accumulation of Fe (229 ± 121 mg/L) in malignant breast tissue samples of patients (p < 0.05) to that in benign tissues samples (49.1 ± 11.4 mg/L). Findings indicated that excess accumulation of iron in malignant tissues can be a risk factor of breast cancer. In order to validate our method of analysis, certified reference material muscle tissue lyophilized (IAEA) MA-M-2/TM was analyzed for metal studied. Determined concentrations were quite in good agreement with certified levels. Asymmetric concentration distribution for Fe, Cu, and Zn was observed in both malignant and benign tissue samples.
authors.affiliation: Chemistry Division, Pakistan Institute of Nuclear Science and Technology, P. O. Nilore, Islamabad, Pakistan, sohailarahman@gmail.com.
authors.forename: Sohaila
authors.initials: S
authors.lastname: Rehman
authors.forename: Syed M
authors.initials: SM
authors.lastname: Husnain
mesh_terms: D001940:Breast / Q000737:chemistry*; D001943:Breast Neoplasms / Q000737:chemistry* / Q000453:epidemiology; D003300:Copper / Q000032:analysis; D005260:Female; D006801:Humans; D007501:Iron / Q000032:analysis; D057230:Limit of Detection; D011336:Probability; D012307:Risk Factors; D013054:Spectrophotometry, Atomic; D015032:Zinc / Q000032:analysis
chemical_list: D003300:Copper; D007501:Iron; D015032:Zinc