title: Electrochemical DNA biosensor for human papillomavirus 16 detection in real samples.
abstract: An electrochemical DNA biosensor for human papillomavirus (HPV) 16 detection has been developed. For this proposed biosensor, L-cysteine was first electrodeposited on the gold electrode surface to form L-cysteine film (CYSFILM). Subsequently, HPV16-specific probe was immobilized on the electrode surface with CYSFILM. Electrochemistry measurement was studied by differential pulse voltammetry method (DPV). The measurement was based on the reduction signals of methylene blue (MB) before and after hybridization either between probe and synthetic target or extracted DNA from clinical samples. The effect of probe concentration was analyzed and the best results were seen at 1000 nM. The hybridization detection presented high sensitivity and broad linear response to the synthetic-target concentration comprised between 18.75 nM and 250 nM as well as to a detection limit of 18.13 nM. The performance of this biosensor was also investigated by checking probe-modified electrode hybridization with extracted DNA from samples. The results showed that the biosensor was successfully developed and exhibited high sensitivity and satisfactory selectivity to HPV16. These results allow for the possibility of developing a new portable detection system for HPVs and for providing help in making an effective diagnosis in the early stages of infection.
authors.affiliation: Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco - UFPE, Av. Prof. Moraes s/n, 50670-901 Recife, PE, Brazil. Electronic address: daniellylsantos@hotmail.com.
authors.forename: Danielly S
authors.initials: DS
authors.lastname: Campos-Ferreira
authors.forename: Gustavo A
authors.initials: GA
authors.lastname: Nascimento
authors.forename: Elaine V M
authors.initials: EV
authors.lastname: Souza
authors.forename: Maria A
authors.initials: MA
authors.lastname: Souto-Maior
authors.forename: Mariana S
authors.initials: MS
authors.lastname: Arruda
authors.forename: Deborah M L
authors.initials: DM
authors.lastname: Zanforlin
authors.forename: Marek H F
authors.initials: MH
authors.lastname: Ekert
authors.forename: Danyelly
authors.initials: D
authors.lastname: Bruneska
authors.forename: José L
authors.initials: JL
authors.lastname: Lima-Filho
mesh_terms: D015374:Biosensing Techniques; D055664:Electrochemical Techniques / Q000379:methods*; D005814:Genes, Viral; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D057230:Limit of Detection; D008751:Methylene Blue / Q000737:chemistry
chemical_list: D008751:Methylene Blue
keywords: Electrochemical DNA biosensor; Human papillomavirus 16; Methylene blue; Screen-printed electrode