title: Pathogenicity and transmission of H5N1 avian influenza viruses in different birds.
abstract: In this study, we selected three H5N1 highly pathogenic avian influenza viruses (HPAIVs), A/Goose/Guangdong/1/1996 (clades 0), A/Duck/Guangdong/E35/2012 (clade 2.3.2.1) and A/Chicken/Henan/B30/2012 (clade 7.2) isolated from different birds in China, to investigate the pathogenicity and transmission of the viruses in terrestrial birds and waterfowl. To observe the replication and shedding of the H5N1 HPAIVs in birds, the chickens were inoculated intranasally with 10(6) EID50 of GSGD/1/96, 10(3) EID50 of DkE35 and CkB30, and the ducks and geese were inoculated intranasally with 10(6) EID50 of each virus. Meanwhile, the naive contact groups were set up to detect the transmission of the viruses in tested birds. Our results showed that DkE35 was highly pathogenic to chickens and geese, but not fatal to ducks. It could be detected from all the tested organs, oropharyngeal and cloacal swabs, and could transmit to the naive contact birds. GSGD/1/96 could infect chickens, ducks and geese, but only caused death in chickens. It could transmit to the chickens and ducks, but was not transmittable to geese. CkB30 was highly pathogenic to chickens, low pathogenic to ducks and not pathogenic to geese. It could be transmitted to the naive contact chickens, but not to the ducks or geese. Our findings suggested that H5N1 HPAIVs from different birds show different host ranges and tissue tropisms. Therefore, we should enhance serological and virological surveillance of H5N1 HPAIVs, and pay more attention to the pathogenic and antigenic evolution of these viruses.
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong, China; College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Runyu
authors.initials: R
authors.lastname: Yuan
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong, China; College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Cui
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong, China; College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Zhang
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong, China; College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Cao
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong, China; College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Xiaoke
authors.initials: X
authors.lastname: Liu
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong, China; College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Yinfeng
authors.initials: Y
authors.lastname: Kang
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong, China; College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Yafen
authors.initials: Y
authors.lastname: Song
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong, China; College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Lang
authors.initials: L
authors.lastname: Gong
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong, China; College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China. Electronic address: prjiao@yahoo.com.
authors.forename: Peirong
authors.initials: P
authors.lastname: Jiao
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong, China; College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China. Electronic address: mliao@scau.edu.cn.
authors.forename: Ming
authors.initials: M
authors.lastname: Liao
mesh_terms: D000818:Animals; D002645:Chickens / Q000821:virology*; D002681:China; D004372:Ducks / Q000821:virology*; D005777:Geese / Q000821:virology*; D005814:Genes, Viral / Q000235:genetics; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D005585:Influenza in Birds / Q000401:mortality / Q000635:transmission* / Q000821:virology*; D010802:Phylogeny; D014774:Virulence / Q000235:genetics
keywords: Avian influenza virus; H5N1; Pathogenicity; Transmission