title: [Influence of electroporation on immunogenicity of the DNA vaccine pVAX-tG250FcGB].
abstract: OBJECTIVE
  To investigate the influence of electroporation on the immunogenicity of the DNA vaccine pVAX- tG250FcGB.
  
  
  METHODS
  The DNA vaccine pVAX-tG250FcGB was constructed by inserting the coding gene of tG250 fusion genes into the expression vector pVAX. The DNA vaccine was delivered in BALB/c mouse by electroporation or intramuscular injection, and the induced antigen specific immune responses were compared.
  
  
  RESULTS
  The vaccine delivered by electroporation and intramuscular injection both induced immune responses in BALB/c mouse, but electroporation produced an obviously stronger effect than intramuscular injection.
  
  
  CONCLUSIONS
  Electroporation-mediated DNA vaccine delivery can produce strong immune response in mice and is an effective means for studying the immunogenic effect of DNA vaccine pVAX-tG250FcGB.
authors.affiliation: Department of Urology, First Affiliated Hospital of General Hospital of PLA, Beijing 100048, China.E-mail: xiaoyi1007@gmail.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xiao
authors.forename: Kun
authors.initials: K
authors.lastname: Gao
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.forename: Jinqi
authors.initials: J
authors.lastname: Yan
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuanji
authors.initials: Y
authors.lastname: Xu
authors.forename: Renli
authors.initials: R
authors.lastname: Tian
authors.forename: Zhiyan
authors.initials: Z
authors.lastname: Du
authors.forename: Jiyun
authors.initials: J
authors.lastname: Yu
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000276:immunology; D018274:Electroporation; D050939:Gene Fusion; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics* / Q000276:immunology; D057809:HEK293 Cells; D006801:Humans; D007273:Injections, Intramuscular; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D011897:Random Allocation; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D014162:Transfection; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*
chemical_list: D000951:Antigens, Neoplasm; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA; C400566:antigen G250; D016178:Granulocyte-Macrophage Colony-Stimulating Factor