title: L-stepholidine, a natural dopamine receptor D1 agonist and D2 antagonist, inhibits heroin-induced reinstatement.
abstract: L-Stepholidine (l-SPD), an alkaloid extract of the Chinese herb Stephania intermedia, is the first compound known to exhibit mixed dopamine D1 receptor agonist/D2 antagonist properties and is a potential medication for the treatment of opiate addiction. The aim of the present study was to investigate the effects of pretreatment with L-SPD on heroin-seeking behavior induced by heroin priming. Male Sprague-Dawley rats were trained to self-administer heroin (0.05mg/kg per infusion) under a fixed ratio 1 schedule for 12 consecutive days and nose-poke responding was extinguished for 12 days, after which reinstatement of drug seeking was induced by heroin priming. Pretreatment with L-SPD (2.5, 5.0 and 10.0mg/kg, i.p.) inhibited the heroin-induced reinstatement of heroin-seeking behavior. Importantly, L-SPD did not affect locomotion, indicating that the observed effects of L-SPD on reinstatement are not the result of motor impairments. The present data suggested that l-SPD inhibits heroin-induced reinstatement and its potential for the treatment of heroin relapse.
authors.affiliation: Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan 430056, China.
authors.forename: Baomiao
authors.initials: B
authors.lastname: Ma
authors.affiliation: Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan 430056, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yue
authors.affiliation: Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan 430056, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan 430056, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Tian
authors.affiliation: Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan 430056, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Ru
authors.affiliation: Drug Prevention and Education Center, Hubei Public Security Bureau, Wuhan 430070, China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Gan
authors.affiliation: Drug Prevention and Education Center, Hubei Public Security Bureau, Wuhan 430070, China.
authors.forename: Daisong
authors.initials: D
authors.lastname: Wang
authors.affiliation: Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan 430056, China; Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201213, China.
authors.forename: Guozhang
authors.initials: G
authors.lastname: Jin
authors.affiliation: Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan 430056, China. Electronic address: licy.whibs.corresp@gmail.com.
authors.forename: Chaoying
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D001599:Berberine / Q000031:analogs & derivatives* / Q000494:pharmacology; D018491:Dopamine Agonists / Q000494:pharmacology; D018492:Dopamine Antagonists / Q000494:pharmacology; D065127:Dopamine D2 Receptor Antagonists; D005108:Extinction, Psychological / Q000187:drug effects* / Q000502:physiology; D003932:Heroin / Q000008:administration & dosage*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017447:Receptors, Dopamine D1 / Q000819:agonists* / Q000502:physiology; D017448:Receptors, Dopamine D2 / Q000502:physiology; D012054:Reinforcement, Psychology; D012646:Self Administration
chemical_list: D018491:Dopamine Agonists; D018492:Dopamine Antagonists; D065127:Dopamine D2 Receptor Antagonists; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D001599:Berberine; C010513:stepholidine; D003932:Heroin
keywords: Dopamine receptor; Heroin; Relapse; Self-administration; l-Stepholidine