title: A novel site for streptomycin resistance in the "530 loop" of chloroplast 16S ribosomal RNA.
abstract: The chloroplast gene for 16S rRNA was cloned from two maternally inherited streptomycin-resistant mutants ofNicotiana differing in degree of resistance at the whole plant and isolated chloroplast level. A single-nucleotide change in the 16S rRNA gene was detected for each mutant: a C to T transition at nucleotide 860 (Escherichia coli coordinate C912) which is an often mutated site, and a novel transition of C to T at nucleotide 472 (E. coli coordinate C525). The novel mutation is located in the phylogenetically conserved "530 loop".
authors.affiliation: Department of Plant Genetics, Weizmann Institute of Science, 76100, Rehovot, Israel.
authors.forename: H
authors.initials: H
authors.lastname: Fromm
authors.forename: E
authors.initials: E
authors.lastname: Galun
authors.forename: M
authors.initials: M
authors.lastname: Edelman