title: High spontaneous granulocyte/macrophage-colony formation in patients with myelofibrosis.
abstract: Unstimulated methylcellulose cultures in 25 myelofibrosis (MF) patients were performed to better understand the role of cytokines in the proliferation of MF cells. Compared to controls MF patients show a variable but highly increased spontaneous CFU-GM formation (66 vs 4.8/10(5) PBMNC). There was a marked reduction of autonomous CFU-GM growth by the cytokine-synthesis-inhibiting molecule IL-10 as well as by antibodies against GM-CSF whereas antibodies against IL-3, G-CSF, M-CSF and IL-1β showed heterogeneous effects. Spontaneous CFU-GM growth >100/10(5) PBMNC predicted shorter survival. Constitutive release of GM-CSF seems to contribute to proliferation of MF cells in vitro and possibly in vivo.
authors.affiliation: 5th Department of Internal Medicine - Oncology/Hematology, Hospital Hietzing, Vienna, Austria.
authors.forename: Verena
authors.initials: V
authors.lastname: Hauer
authors.affiliation: Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria.
authors.forename: Eva
authors.initials: E
authors.lastname: Jäger
authors.affiliation: 5th Department of Internal Medicine - Oncology/Hematology, Hospital Hietzing, Vienna, Austria.
authors.forename: Thamer
authors.initials: T
authors.lastname: Sliwa
authors.affiliation: 5th Department of Internal Medicine - Oncology/Hematology, Hospital Hietzing, Vienna, Austria.
authors.forename: Ralph
authors.initials: R
authors.lastname: Simanek
authors.affiliation: Department of Internal Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna, Vienna, Austria.
authors.forename: Heinz
authors.initials: H
authors.lastname: Gisslinger
authors.affiliation: Department of Internal Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna, Vienna, Austria.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Jäger
authors.affiliation: 5th Department of Internal Medicine - Oncology/Hematology, Hospital Hietzing, Vienna, Austria; Ludwig Boltzmann Institute for Clinical Oncology, Vienna, Austria. Electronic address: klaus.geissler@wienkav.at.
authors.forename: Klaus
authors.initials: K
authors.lastname: Geissler
mesh_terms: D057134:Antibodies, Neutralizing / Q000276:immunology / Q000494:pharmacology; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay / Q000379:methods*; D016179:Granulocyte Colony-Stimulating Factor / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D055014:Granulocyte-Macrophage Progenitor Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D016753:Interleukin-10 / Q000378:metabolism / Q000494:pharmacology; D053583:Interleukin-1beta / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D007377:Interleukin-3 / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D053208:Kaplan-Meier Estimate; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D016173:Macrophage Colony-Stimulating Factor / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D055728:Primary Myelofibrosis / Q000097:blood / Q000378:metabolism / Q000473:pathology*
chemical_list: D057134:Antibodies, Neutralizing; D053583:Interleukin-1beta; D007377:Interleukin-3; D016753:Interleukin-10; D016179:Granulocyte Colony-Stimulating Factor; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor
keywords: CFU-GM; Cultures; Methylcellulose; Myelofibrosis; Spontaneous