title: Preparation and antioxidant activity of Lycium barbarum oligosaccharides.
abstract: In this study, the Lycium barbarum oligosaccharides (LBO) were prepared by hydrolysis using hydrogen peroxide (H₂O₂). The yield of the LBO was monitored during the hydrolysis process. The hydrolysis conditions were optimized as follows: time, 4h; temperature, 70 °C; and H₂O₂ concentration, 2.5% (v/v). The hydrolysates were filtered, concentrated to ∼20% (w/v), precipitated with 6 volumes of absolute ethanol, freeze-dried, and ground to yield a water soluble and white powder. The sugar content of the product was 95.8%, and the yield was 21.05% (w/w), respectively. The LBO show higher hydroxyl radical scavenging activity (86.46%) than Vc (40.96) at the concentration of 100 μg/mL.
authors.affiliation: School of Food Engineering, Huaihai Institute of Technology, 59 Cangwu Road, Xinpu 222005, China. Electronic address: jianglongfa008@sina.com.
authors.forename: Long-Fa
authors.initials: LF
authors.lastname: Jiang
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006868:Hydrolysis; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors*; D032305:Lycium / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D010084:Oxidation-Reduction; D011208:Powders; D012995:Solubility; D014867:Water / Q000737:chemistry
chemical_list: D000975:Antioxidants; D009844:Oligosaccharides; D011208:Powders; D014867:Water; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide
keywords: Antioxidant activity; Hydrogen peroxide; Lycium barbarum oligosaccharides