title: Xylogranin B: a potent Wnt signal inhibitory limonoid from Xylocarpus granatum.
abstract: Xylogranin B (2) was isolated from Xylocarpus granatum (Meliaceae) leaves, by use of a cell-based luciferase screening system targeting a Wnt signaling pathway. Compound 2 inhibited TCF/β-catenin transcriptional activity (IC50 48.9 nM) and exhibited strong cytotoxicity against colon cancer cell lines. Compound 2 significantly decreased β-catenin protein levels in nuclei but not in the cytosol. These results indicated that a decrease in β-catenin levels in nuclei by 2 resulted in the Wnt signal inhibitory effects of 2.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Chiba University , 1-8-1 Inohana, Chuo-ku, Chiba 260-8675, Japan , Pharmacy Discipline, Life Science School, Khulna University , Khulna 9208, Bangladesh , and Department of Pharmaceutical Chemistry, University of Dhaka , Dhaka 1000, Bangladesh.
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Toume
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kamiya
authors.forename: Midori A
authors.initials: MA
authors.lastname: Arai
authors.forename: Naomi
authors.initials: N
authors.lastname: Mori
authors.forename: Samir K
authors.initials: SK
authors.lastname: Sadhu
authors.forename: Firoj
authors.initials: F
authors.lastname: Ahmed
authors.forename: Masami
authors.initials: M
authors.lastname: Ishibashi
mesh_terms: D003110:Colonic Neoplasms / Q000188:drug therapy; D004354:Drug Screening Assays, Antitumor; D005663:Furans; D045325:HCT116 Cells; D006801:Humans; D036701:Limonins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008156:Luciferases / Q000378:metabolism; D029582:Meliaceae / Q000737:chemistry*; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry; D060449:Wnt Signaling Pathway / Q000187:drug effects*; D051176:beta Catenin / Q000187:drug effects
chemical_list: D005663:Furans; D036701:Limonins; D014315:Triterpenes; D051176:beta Catenin; C516646:xylogranatin B; C092741:methyl angolensate; D008156:Luciferases