title: Cytotoxic ent-kaurane diterpenoids from Salvia cavaleriei.
abstract: Fifteen new ent-kaurane diterpenoids, compounds 1-15, and two known analogues, 4-epi-henryine A (16) and leukamenin E (17), were isolated from the whole plants of Salvia cavaleriei. The structures of the new compounds were established by spectroscopic methods, and their absolute configurations were determined by electronic circular dichroism and single-crystal X-ray diffraction analyses with Cu Kα radiation. Compounds 1-15 were evaluated for their cytotoxicity against five human cancer cell lines, HL-60, SMMC-7721, A-549, MCF-7, and SW480, as well as the noncancerous Beas-2B cell line. Compounds 1-10, 12, 14, and 15 showed broad-spectrum cytotoxicity, with compounds 1, 3, 6-10, 12, and 15 exhibiting more potent cytotoxicity than the positive control, cis-platin, with IC50 values ranging from 0.65 to 6.4 μM.
authors.affiliation: Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430030, People's Republic of China.
authors.forename: Heng
authors.initials: H
authors.lastname: Zheng
authors.forename: Qiong
authors.initials: Q
authors.lastname: Chen
authors.forename: Mengke
authors.initials: M
authors.lastname: Zhang
authors.forename: Yongji
authors.initials: Y
authors.lastname: Lai
authors.forename: Liang
authors.initials: L
authors.lastname: Lei
authors.forename: Penghua
authors.initials: P
authors.lastname: Shu
authors.forename: Jinwen
authors.initials: J
authors.lastname: Zhang
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Xue
authors.forename: Zengwei
authors.initials: Z
authors.lastname: Luo
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Guangmin
authors.initials: G
authors.lastname: Yao
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002942:Circular Dichroism; D002945:Cisplatin / Q000494:pharmacology; D018360:Crystallography, X-Ray; D045786:Diterpenes, Kaurane / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005260:Female; D018922:HL-60 Cells; D006801:Humans; D061986:MCF-7 Cells; D008968:Molecular Conformation; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D027544:Salvia / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D045786:Diterpenes, Kaurane; D004365:Drugs, Chinese Herbal; D002945:Cisplatin