title: Osteogenesis induction of periodontal ligament cells onto bone morphogenic protein-2 immobilized PCL fibers.
abstract: The purpose of this study was to develop bone morphogenic protein-2 (BMP-2) immobilized PCL fibers to induce osteogenic differentiation of periodontal ligament cells (PDLCs). The PCL fiber surface was modified with heparin-dopamine (Hep-DOPA) (Hep-PCL) and further immobilized with BMP-2 (BMP-2/Hep-PCL). PCL fibers and surface-modified PCL fibers (Hep-PCL and BMP-2/Hep-PCL) were characterized by X-ray photoelectron spectroscopy (XPS) and contact angle. Osteogenic differentiation of PDLCs was demonstrated by alkaline phosphatase (ALP) activity, calcium deposition, and gene expression. The results of XPS and contact angle revealed that Hep-DOPA and BMP-2 were successfully immobilized onto the PCL surface and that the BMP-2/Hep-PCL fibers have more hydrophilic surface properties than PCL fibers alone. ALP activity, calcium deposition, and gene expression on BMP-2/Hep-PCL fibers showed significantly induced osteogenic differentiation relative to PCL fibers. Therefore, we suggest that BMP-2/Hep-PCL fibers have the potential to effectively induce osteogenic differentiation of PDLCs.
authors.affiliation: Department of Orthopedic Surgery & Rare Diseases Institute, Korea University Medical Center, Guro Hospital, #148, Gurodong-ro, Guro-gu, Seoul 152-703, Republic of Korea.
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Kim
authors.forename: Young-Pil
authors.initials: YP
authors.lastname: Yun
authors.forename: Yun-Kyung
authors.initials: YK
authors.lastname: Han
authors.forename: Deok-Won
authors.initials: DW
authors.lastname: Lee
authors.forename: Joo-Young
authors.initials: JY
authors.lastname: Ohe
authors.forename: Baek-Soo
authors.initials: BS
authors.lastname: Lee
authors.forename: Hae-Ryong
authors.initials: HR
authors.lastname: Song
authors.forename: Kyeongsoon
authors.initials: K
authors.lastname: Park
authors.forename: Byung-Joon
authors.initials: BJ
authors.lastname: Choi
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D004298:Dopamine / Q000737:chemistry* / Q000494:pharmacology; D015870:Gene Expression; D006493:Heparin / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D055767:Immobilized Proteins; D010012:Osteogenesis / Q000187:drug effects; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D056951:Photoelectron Spectroscopy; D011091:Polyesters / Q000737:chemistry*; D061251:Primary Cell Culture; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D055767:Immobilized Proteins; D011091:Polyesters; C016240:polycaprolactone; D006493:Heparin; D000469:Alkaline Phosphatase; D002118:Calcium; D004298:Dopamine
keywords: Bone morphogenic protein-2 (BMP-2); Osteogenesis induction; PCL fiber; Periodontal ligament cells (PDLCs)