title: Pigment-pigment interactions and secondary structure of reconstituted algal chlorophyll a/b-binding light-harvesting complexes of Chlorella fusca with different pigment compositions and pigment-protein stoichiometries.
abstract: Earlier we have shown by in vitro reconstitution experiments that the pigment composition of the chlorophyll alb-binding light-harvesting complex of the green alga Chlorella fusca could be altered in a relatively broad range (Meyer and Wilhelm 1993). In this study we used these reconstituted complexes of different pigment loading to analyze the excitonic interactions between the pigment molecules and the secondary structure by means of circular dichroism spectra in the visible and the far UV spectral regions, respectively. We found that, in contrast to the expectations, the pigment composition and pigment content hardly affected the circular dichroism spectra in the visible spectral region. Reconstituted complexes, independent of their pigment composition, exhibited the most characteristic circular dichroism bands of the native light-harvesting complex, even if one polypeptide bound only 3 chlorophyll a, 3 chlorophyll b and 1-2 xanthophyll molecules. Full restoration of the protein secondary structure, however, could not be achieved. The α-helix content depended significantly on the pigment composition as well as on the pigment-protein ratio of the reconstituted complexes. Further binding of pigments resulted in restoration of the minor excitonic circular dichroism bands, the amplitudes of which depended on the pigment content of the reconstituted complexes. These data suggest that in the reconstitution of light-harvesting complexes a 'central cluster' of pigment molecules plays an important role. Further binding of pigments to the 'peripheral binding sites' appeared also to stabilize the protein secondary structure of the reconstituted complexes.
authors.affiliation: Institute of General Botany, University of Mainz, 55099, Mainz, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Meyer
authors.forename: C
authors.initials: C
authors.lastname: Wilhelm
authors.forename: G
authors.initials: G
authors.lastname: Garab