title: Temperature dependence of intracellular pH in higher plant cells : A (31)P nuclear magnetic resonance study on maize root tips.
abstract: The recent introduction of (31)P nuclear magnetic resonance spectroscopy offers a new approach to the problem of obtaining a simultaneous and direct evaluation of both the cytoplasmic and vacuolar pH in higher plant cells (J. K. M. Roberts, P.M. Ray, N. Waderlardetzky and O. Sardetzky, 1980, Nature 283, 870-872; 1981, Planta 152, 74-78). Using this method we have been able to detect a selective pH decrease of about 0.5 units at the level of the cytoplasmic compartment of maize root tips when the temperature was increased from 4 to 28°C. This effect was completely reversible with temperature. No pH variation could be detected at the level of the vacuolar compartment.
authors.affiliation: Gruppo di Chimica Biologica e Strutturistica Chimica, Instituto di Chimica Biologica, Università di Roma, Piazzale Aldo Moro 5, I-00185, Roma, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Aducci
authors.forename: R
authors.initials: R
authors.lastname: Federico
authors.forename: G
authors.initials: G
authors.lastname: Carpinelli
authors.forename: F
authors.initials: F
authors.lastname: Podo