title: Fast profiling ecotoxicity and skin permeability of benzophenone ultraviolet filters using biopartitioning micellar chromatography based on penetrable silica spheres.
abstract: Penetrable silica possesses hierarchical pores, mesopores and penetrable macropores, offering fast mass transfer, satisfactory mechanical strength as well as low column pressure. In the present study, penetrable octadecyl-bonded silica (ODS) was for the first time used as biopartitioning micellar chromatography (BMC) stationary phase to profile ecotoxicity and skin permeability of benzophenone UV-filters. Mobile phase (MP) pH and concentration of polyoxyethylene(23)lauryl ether in the MP were systematically studied. Quantitative retention-activity relationships (QRARs) model was established to correlate retention factors (k) on BMC with bioconcentration factor (BCF) and transdermal rate (TR) of UV-filters. Coefficient of determination (r(2)) of the QRARs model between log BCF and log k were 0.9398-0.9753, while r(2) between TR and log k were 0.7569-0.8434, which demonstrated satisfactory predictive ability of the methodology. It was a powerful tool for fast screening by combining penetrable ODS with BMC, and avoiding column blockage often occurring in BMC.
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Chen-ru
authors.initials: CR
authors.lastname: Yin
authors.forename: Li-yun
authors.initials: LY
authors.lastname: Ma
authors.forename: Jian-geng
authors.initials: JG
authors.lastname: Huang
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.forename: Zhi-guo
authors.initials: ZG
authors.lastname: Shi
mesh_terms: D001577:Benzophenones / Q000633:toxicity*; D002853:Chromatography, Liquid / Q000379:methods*; D008823:Micelles; D010539:Permeability; D012822:Silicon Dioxide / Q000737:chemistry*; D012867:Skin / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D001577:Benzophenones; D008823:Micelles; C047723:benzophenone; D012822:Silicon Dioxide
keywords: Benzophenone ultraviolet filters; Biopartitioning micellar chromatography; Penetrable silica; Quantitative retention–activity relationships; Skin permeability