title: Comparative study of endophytic and endophytic diazotrophic bacterial communities across rice landraces grown in the highlands of northern Thailand.
abstract: Communities of bacterial endophytes within the rice landraces cultivated in the highlands of northern Thailand were studied using fingerprinting data of 16S rRNA and nifH genes profiling by polymerase chain reaction-denaturing gradient gel electrophoresis. The bacterial communities' richness, diversity index, evenness, and stability were varied depending on the plant tissues, stages of growth, and rice cultivars. These indices for the endophytic diazotrophic bacteria within the landrace rice Bue Wah Bo were significantly the lowest. The endophytic bacteria revealed greater diversity by cluster analysis with seven clusters compared to the endophytic diazotrophic bacteria (three clusters). Principal component analysis suggested that the endophytic bacteria showed that the community structures across the rice landraces had a higher stability than those of the endophytic diazotrophic bacteria. Uncultured bacteria were found dominantly in both bacterial communities, while higher generic varieties were observed in the endophytic diazotrophic bacterial community. These differences in bacterial communities might be influenced either by genetic variation in the rice landraces or the rice cultivation system, where the nitrogen input affects the endophytic diazotrophic bacterial community.
authors.affiliation: Microbiology Division, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand, rangjaroen@gmail.com.
authors.forename: Chakrapong
authors.initials: C
authors.lastname: Rangjaroen
authors.forename: Benjavan
authors.initials: B
authors.lastname: Rerkasem
authors.forename: Neung
authors.initials: N
authors.lastname: Teaumroong
authors.forename: Rungroch
authors.initials: R
authors.lastname: Sungthong
authors.forename: Saisamorn
authors.initials: S
authors.lastname: Lumyong
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics; D018407:Bacterial Physiological Phenomena; D044822:Biodiversity; D004269:DNA, Bacterial / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D060026:Endophytes / Q000145:classification / Q000235:genetics*; D014644:Genetic Variation; D012275:Oryza / Q000382:microbiology*; D010088:Oxidoreductases / Q000235:genetics; D016133:Polymerase Chain Reaction; D025341:Principal Component Analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013785:Thailand
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; C029130:nitrogenase reductase