title: Increased serum levels and chondrocyte expression of nesfatin-1 in patients with osteoarthritis and its relation with BMI, hsCRP, and IL-18.
abstract: BACKGROUND
  Adipokines have been proved to relate with osteoarthritis (OA). As a recently discovered adipokine, nesfatin-1 relationship with OA has not been reported.
  
  
  OBJECTIVE
  To determine the levels of nesfatin-1 in serum and synovial fluid (SF) from patients with and without OA; to examine the correlation between nesfatin-1 levels and high sensitivity C-reactive protein (hsCRP), Type IIA Collagen N Propeptide (PIIANP), and IL-18 (interleukin-18) levels in serum or synovial fluid.
  
  
  METHODS
  Serum and SF were collected from knee OA patients and healthy persons, respectively. Five articular tissues were obtained during TKR for immunohistochemistry (IHC). Nesfatin-1 levels, hsCRP, PIIANP, and IL-18 in serum and SF were analyzed by enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  Nesfatin-1 gene was expressed in OA-affected articular cartilage. OA serum contained significantly higher levels of nesfatin-1, as compared to serum from healthy controls (P < 0.05), and nesfatin-1 levels in OA serum exceeded those in paired SF samples (P < 0.001). Significant correlation was found between serum nesfatin-1 and hsCRP levels in OA patients (r = 0.593, P = 0.00005) and also synovial nesfatin-1 and IL-18 levels (r = 0.560, P = 0.0017).
  
  
  CONCLUSIONS
  Nesfatin-1 is present in articular tissues and may contribute to the physiopathologic changes in OA. Nesfatin-1, accompanied with hsCRP and IL-18, could be new molecular makers to speculate OA progression.
authors.affiliation: Department of Orthopedics Surgery, The Second Hospital of Medical College, Zhejiang University, Hangzhou 310000, China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Jiang
authors.forename: Jiapeng
authors.initials: J
authors.lastname: Bao
authors.forename: Xindie
authors.initials: X
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiong
authors.forename: Lidong
authors.initials: L
authors.lastname: Wu
mesh_terms: D000368:Aged; D019645:Arthroplasty, Replacement, Knee; D015992:Body Mass Index; D002097:C-Reactive Protein / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000097:blood*; D002356:Cartilage / Q000473:pathology; D016022:Case-Control Studies; D019902:Chondrocytes / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000097:blood*; D018450:Disease Progression; D005260:Female; D005265:Femoral Neck Fractures / Q000473:pathology; D005786:Gene Expression Regulation; D006801:Humans; D020382:Interleukin-18 / Q000097:blood*; D007717:Knee / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000097:blood*; D000080866:Nucleobindins; D020370:Osteoarthritis, Knee / Q000097:blood* / Q000378:metabolism*; D054850:Osteophyte; D010446:Peptide Fragments / Q000378:metabolism; D011347:Procollagen / Q000378:metabolism; D013582:Synovial Fluid / Q000378:metabolism; D013583:Synovial Membrane / Q000473:pathology
chemical_list: D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D020382:Interleukin-18; C000630650:NUCB2 protein, human; D009419:Nerve Tissue Proteins; D000080866:Nucleobindins; D010446:Peptide Fragments; D011347:Procollagen; C109195:procollagen type IIA amino-terminal peptide; D002097:C-Reactive Protein