title: [Effect of Porphyromonas gingivalis lipopolysacchearide on the expression of CC chemokine receptor 2 in monocytes].
abstract: OBJECTIVE
  To investigate the effects of Porphyromonas gingivalis lipopolysacchearide (Pg-LPS) on the expression of CC chemokine receptor 2 (CCR2) in THP-1 monocyte and to explore the relationship between periodontitis and cardiovascular disease in molecular level.
  
  
  METHODS
  THP-1 monocytes were incubated with different concentrations of Pg-LPS (10, 100, 1000 µg/L) for 1, 4 and 24 h respectively, then flow cytometry and reverse transcription-PCR were adopted to determine cell surface protein levels and mRNA levels of CCR2.
  
  
  RESULTS
  The protein levels and mRNA levels of CCR2 were higher in all experiment groups of 1 h and 4 h than that in the control group (P < 0.05) , except the protein expression of CCR2 in T1 group of 1 h (55.74 ± 0.96) . The protein expression (52.56 ± 0.61, 40.98 ± 0.86, 26.50 ± 0.67) and mRNA levels (0.095 ± 0.006,0.070 ± 0.004,0.046 ± 0.004) of CCR2 were lower in all experiment groups than that in the control group (56.99 ± 0.44,0.104 ± 0.003) at 24 h (P < 0.05) . The protein levels and mRNA levels of CCR2 were increased in all experiment groups at 4 h and reduced at 24 h (P < 0.05).
  
  
  CONCLUSIONS
  Pg-LPS can upregulate CCR2 expression on THP-1 monocyte surface in concentration dependent manner in early stage, promoting the monocyte chemoattractant. Periodontitis may promote atherosclerosis by enhancing monocyte chemoattractant through periodontal pathogens.
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.forename: Yue
authors.initials: Y
authors.lastname: Jia
authors.forename: Jia-jun
authors.initials: JJ
authors.lastname: Chen
authors.forename: Ya-fei
authors.initials: YF
authors.lastname: Wu
mesh_terms: D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D016966:Porphyromonas gingivalis / Q000737:chemistry*; D012333:RNA, Messenger / Q000378:metabolism; D054390:Receptors, CCR2 / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D015854:Up-Regulation
chemical_list: C515334:CCR2 protein, human; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D054390:Receptors, CCR2