title: Do gene expression changes in articular cartilage proteases of the synovial membrane correlate with expression changes of the same genes in systemic blood cells?
abstract: OBJECTIVE
  The aim of our study was to find whether an injury of the knee joint tissues increases gene expression of selected hyaline cartilage degenerating enzymes such as matrix metaloproteinases (MMP) and aggreacaneses (Agg).
  
  
  METHODS
  A total of 138 patients (81 female, 57 male) were admitted for knee arthroscopy with a mean age of 38.8 years. Full blood samples were collected preoperatively and synovium samples intraoperatively. Joint tissue lesions such as menisci, anterior cruciate ligament (ACL) and hyaline cartilage were estimated. Real time PCR with spectrophotometric analysis was performed.
  
  
  RESULTS
  An ACL lesion was found in 56 patients, medial menisci (MM) in 65, and lateral menisci (LM) in five. Chondral lesions were estimated according to Outerbridge's grading system. In laboratory tests correlation between ACL tear and gene expression was seen except TIMP1 in serum (p < 0.05). In MM lesions MMP9, Agg2 elevation in serum was observed. LM lesions erased MMP13, MMP14 in serum and MMP8 in synovium. Chondral lesions revealed that many genes had higher expression in patients without hyaline degeneration. All of the gene expressions correlated between serum and synovium.
  
  
  CONCLUSIONS
  An ACL lesion provokes elevation in expression of proteases genes, while the influence of other lesions remains elusive. Gene expression in synovium correlates with peripheral blood.
authors.affiliation: Department of Orthopaedics and Paediatric Orthopaedics, Medical University of Lodz, ul. Drewnowska 75, 91-002, Lodz, Poland, kfasmail@wp.pl.
authors.forename: Adam
authors.initials: A
authors.lastname: Kwapisz
authors.forename: Michał
authors.initials: M
authors.lastname: Chojnacki
authors.forename: Marcin
authors.initials: M
authors.lastname: Domżalski
authors.forename: Andrzej
authors.initials: A
authors.lastname: Grzegorzewski
authors.forename: Marek
authors.initials: M
authors.lastname: Synder
mesh_terms: D000328:Adult; D016118:Anterior Cruciate Ligament / Q000378:metabolism / Q000473:pathology; D001182:Arthroscopy; D002358:Cartilage, Articular / Q000201:enzymology*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007718:Knee Injuries / Q000378:metabolism* / Q000601:surgery; D007963:Leukocytes, Mononuclear / Q000378:metabolism* / Q000473:pathology; D008297:Male; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism*; D008592:Menisci, Tibial / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D013583:Synovial Membrane / Q000378:metabolism* / Q000473:pathology; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D010450:Endopeptidases; D020782:Matrix Metalloproteinases; C083641:aggrecanase