title: Isolation and reconstitution of the RNA replicase of the cytoplasmic polyhedrosis virus of silkworm,Bombyx mori.
abstract: After irradiation of the virus particles of CPV, the RNA replicase associated with the virion was isolated in the form of a genome-replicase complex with DEAE-Sephadex A-25 chromatography. This complex was then treated with Triton X-100 and purified by phosphocellulose column chromatography. The RNA replicase reconstituted with the doublestranded RNA of CPV showed both the enzyme activity of RNA polymerase and methyltransferase. The single-stranded RNA could not serve as the template for the RNA replicase. The role of the RNA replicase of CPV is discussed.
authors.affiliation: Shanghai Institute of Biochemistry, Academia Sinica, Shanghai, China.
authors.forename: W
authors.initials: W
authors.lastname: Ai-Zhen
authors.forename: S
authors.initials: S
authors.lastname: Yu-Kun