title: A novel method for discrimination of beef and horsemeat using Raman spectroscopy.
abstract: A new approach, based on the usage of Raman spectroscopy in combination with chemometrics, was developed for the rapid determination of beef adulteration with horsemeat. The data mining process of collected Raman spectra was performed with principal component analysis (PCA). Pure fat samples, extracted from forty-nine meat beef and horsemeat samples, were analysed using the Raman spectroscopy. All meat samples were classified successfully according to their origins. The presence of different concentrations (25%, 50%, 75%, w/w) of horsemeat in beef was also differentiated using the developed model system. This study offers a rapid assay for determination of meat adulteration by discriminating beef and horsemeat with high accuracy, a short analysis time (30s) and no requirement for time-consuming sample preparation procedures.
authors.affiliation: Department of Food Engineering, Faculty of Engineering, Hacettepe University, Beytepe, 06800 Ankara, Turkey; Food Research Center, Hacettepe University, Beytepe, 06800 Ankara, Turkey. Electronic address: ihb@hacettepe.edu.tr.
authors.forename: İsmail Hakkı
authors.initials: İH
authors.lastname: Boyacı
authors.forename: Havva Tümay
authors.initials: HT
authors.lastname: Temiz
authors.forename: Reyhan Selin
authors.initials: RS
authors.lastname: Uysal
authors.forename: Hasan Murat
authors.initials: HM
authors.lastname: Velioğlu
authors.forename: Reza Jafarzadeh
authors.initials: RJ
authors.lastname: Yadegari
authors.forename: Mojtaba Mahmoudi
authors.initials: MM
authors.lastname: Rishkan
mesh_terms: D000818:Animals; D002417:Cattle; D016002:Discriminant Analysis; D005506:Food Contamination / Q000032:analysis*; D006736:Horses; D008460:Meat / Q000032:analysis*; D025341:Principal Component Analysis; D013059:Spectrum Analysis, Raman / Q000379:methods*
keywords: Adulteration; Beef; Horsemeat; Principal component analysis; Raman spectroscopy