title: Responses of glutathione-related antioxidant defense system in serum of Nile tilapia (Oreochromis niloticus) exposed to sublethal concentration of methomyl and recovery pattern.
abstract: Tilapia were exposed to sublethal concentrations of 0, 0.2, 2, 20, or 200 μg/L for 30 days, and then transferred to methomyl-free water for 18 days. GST, GPx, GR, GSH, and GSSG in tilapia serum were examined at 0, 6, 12, 18, 24, and 30 days after methomyl exposure and at 18 days after transferring to methomyl-free water. There were no significant changes in antioxidants activities and contents in serum of tilapia exposed to 0.2 μg/L. Significant increases in GST, GR, GPx, and GSSG accompanied by a decrease in GSH were observed following methomyl exposure to 2, 20, or 200 μg/L, suggesting the presence of oxidative stress. Thus, it would appear the 0.2 μg/L methomyl might be considered the no observed adverse effect level. Recovery data showed that the effects produced by lower concentration of 20 μg/L were reversible but not at the higher 200 μg/L concentration.
authors.affiliation: Department of Fishery Environment Protection, Wuxi Fishery College, Nanjing Agricultural University, Wuxi, 214081, China; Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Wuxi, 214081, China.
authors.forename: Shun-Long
authors.initials: SL
authors.lastname: Meng
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Qu
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Fan
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Qiu
authors.forename: Jia-Zhang
authors.initials: JZ
authors.lastname: Chen
authors.forename: Pao
authors.initials: P
authors.lastname: Xu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D023681:Cichlids / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D007306:Insecticides / Q000633:toxicity*; D008297:Male; D008724:Methomyl / Q000633:toxicity*; D019055:No-Observed-Adverse-Effect Level; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D007306:Insecticides; D017382:Reactive Oxygen Species; D008724:Methomyl; D005978:Glutathione
keywords: antioxidants; methomyl; recovery; serum; tilapia