title: Novel parallelized quadrupole/linear ion trap/Orbitrap tribrid mass spectrometer improving proteome coverage and peptide identification rates.
abstract: Proteome coverage and peptide identification rates have historically advanced in line with improvements to the detection limits and acquisition rate of the mass spectrometer. For a linear ion trap/Orbitrap hybrid, the acquisition rate has been limited primarily by the duration of the ion accumulation and analysis steps. It is shown here that the spectral acquisition rate can be significantly improved through extensive parallelization of the acquisition process using a novel mass spectrometer incorporating quadrupole, Orbitrap, and linear trap analyzers. Further, these improvements to the acquisition rate continue to enhance proteome coverage and general experimental throughput.
authors.affiliation: Thermo Fisher Scientific , 355 River Oaks Parkway, San Jose, California 95134, United States.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Senko
authors.forename: Philip M
authors.initials: PM
authors.lastname: Remes
authors.forename: Jesse D
authors.initials: JD
authors.lastname: Canterbury
authors.forename: Raman
authors.initials: R
authors.lastname: Mathur
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Song
authors.forename: Shannon M
authors.initials: SM
authors.lastname: Eliuk
authors.forename: Chris
authors.initials: C
authors.lastname: Mullen
authors.forename: Lee
authors.initials: L
authors.lastname: Earley
authors.forename: Mark
authors.initials: M
authors.lastname: Hardman
authors.forename: Justin D
authors.initials: JD
authors.lastname: Blethrow
authors.forename: Huy
authors.initials: H
authors.lastname: Bui
authors.forename: August
authors.initials: A
authors.lastname: Specht
authors.forename: Oliver
authors.initials: O
authors.lastname: Lange
authors.forename: Eduard
authors.initials: E
authors.lastname: Denisov
authors.forename: Alexander
authors.initials: A
authors.lastname: Makarov
authors.forename: Stevan
authors.initials: S
authors.lastname: Horning
authors.forename: Vlad
authors.initials: V
authors.lastname: Zabrouskov
mesh_terms: D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors
chemical_list: D010455:Peptides