title: Genotoxicity assessment of multispecies probiotics using reverse mutation, mammalian chromosomal aberration, and rodent micronucleus tests.
abstract: Genotoxicity assessment is carried out on freeze dried powder of cultured probiotics containing Lactobacillus rhamnosus LCR177, Bifidobacterium adolescentis BA286, and Pediococcus acidilactici PA318. Ames tests, in vitro mammalian chromosome aberration assay, and micronucleus tests in mouse peripheral blood are performed. For 5 strains of Salmonella Typhimurium, the Ames tests show no increased reverse mutation upon exposure to the test substance. In CHO cells, the frequency of chromosome aberration does not increase in responding to the treatment of probiotics. Likewise, the frequency of micronucleated reticulocytes in probiotics-fed mice is indistinguishable from that in the negative control group. Taken together, the toxicity assessment studies suggest that the multispecies probiotic mixture does not have mutagenic effects on various organisms.
authors.affiliation: Department of Food Science and Technology, Hungkuang University, No. 1018, Sector 6, Taiwan Boulevard, Shalu District, Taichung 43302, Taiwan ; New Bellus Enterprises Co. Ltd., Tainan 72042, Taiwan.
authors.forename: Yi-Jen
authors.initials: YJ
authors.lastname: Chiu
authors.forename: Mun-Kit
authors.initials: MK
authors.lastname: Nam
authors.forename: Yueh-Ting
authors.initials: YT
authors.lastname: Tsai
authors.forename: Chun-Chi
authors.initials: CC
authors.lastname: Huang
authors.forename: Cheng-Chih
authors.initials: CC
authors.lastname: Tsai
mesh_terms: D000818:Animals; D001644:Bifidobacterium; D016466:CHO Cells / Q000187:drug effects; D002869:Chromosome Aberrations; D003412:Cricetulus; D052201:Lacticaseibacillus rhamnosus; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015162:Micronucleus Tests; D009152:Mutagenicity Tests / Q000379:methods*; D009154:Mutation; D010376:Pediococcus; D019936:Probiotics / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics