title: Expression of an active spinach acyl carrier protein-I/protein-A gene fusion.
abstract: A synthetic gene encoding spinach acyl carrier protein I (ACP-I) was fused to a gene encoding the Fc-binding portion of staphylococcal protein A. This gene fusion, under the control of the λPR promoter, was expressed at high levels in Escherichia coli producing a 42 kDa fusion protein. This fusion protein was phosphopantethenylated in E. coli. In vitro the ACP portion of the fusion protein was able to participate in acyl ACP synthetase reactions, plant malonyl-CoA:ACP transacylase (MCT) reactions, and plant fatty acid synthetase (FAS) reactions. Inhibitory effects of high ACP concentrations on in vitro plant FAS were observed with the unfused ACP-1 but not with the fusion protein. As with unfused ACP-I, the fusion protein was a poor substrate for E. coli FAS reactions. When injected into rabbits, the fusion protein was also able to generate antiserum to spinach ACP-I.
authors.affiliation: Plant Biochemistry Unit, Agricultural Research Service, U.S. Department of Agriculture, Northern Regional Research Center, 1815 N. University St., 61604, Peoria, IL, USA.
authors.forename: P D
authors.initials: PD
authors.lastname: Beremand
authors.forename: D D
authors.initials: DD
authors.lastname: Elmore
authors.forename: K
authors.initials: K
authors.lastname: Dziewanowska
authors.forename: D J
authors.initials: DJ
authors.lastname: Guerra