title: Evaluation of the potential anti-adhesion effect of the PVA/Gelatin membrane.
abstract: A common and prevailing complication for patients with abdominal surgery is the peritoneal adhesion that follows during the post-operative recovery period. Biodegradable polymers have been suggested as a barrier to prevent the peritoneal adhesion. In this work, as a preventive method, PVA/Gelatin hydrogel-based membrane was investigated with various combinations of PVA and gelatin (50/50, 30/70/, and 10/90). Membranes were made by casting method using hot PVA-gelatin solution and the gelatin was cross-linked by exposing UV irradiation for 5 days to render stability of the produced sheathed form in the physiological environment. Physical crosslinking was chosen to avoid the problems of potential cytotoxic effect of chemical crosslinking. Their materials characterization and mechanical properties were evaluated by SEM surface characterization, hydrophilicity, biodegradation rate, and so forth. Cytocompatibility was observed by in vitro experiments with cell proliferation using confocal laser scanning microscopy and the MTT assay by L-929 mouse fibroblast cells. The fabricated PVA/Gel membranes were implanted between artificially defected cecum and peritoneal wall in rats and were sacrificed after 1 and 2 weeks post-operative to compare their tissue adhesion extents with that of control group where the defected surface was not separated by PVA/Gel membrane. The PVA/Gel membrane (10/90) significantly reduced the adhesion extent and showed to be a potential candidate for the anti-adhesion application.
authors.affiliation: Department of Surgery, College of Medicine, Soonchunhyang University Hospital, Cheonan, Chungnam, 330-090, Republic of Korea.
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Bae
authors.forename: So-Ra
authors.initials: SR
authors.lastname: Son
authors.forename: Swapan
authors.initials: S
authors.lastname: Kumar Sakar
authors.forename: Thi-Hiep
authors.initials: TH
authors.lastname: Nguyen
authors.forename: Shin-Woo
authors.initials: SW
authors.lastname: Kim
authors.forename: Young-Ki
authors.initials: YK
authors.lastname: Min
authors.forename: Byong-Taek
authors.initials: BT
authors.lastname: Lee
mesh_terms: D034861:Abdominal Wall / Q000601:surgery*; D000818:Animals; D001672:Biocompatible Materials; D002432:Cecum / Q000601:surgery*; D002448:Cell Adhesion; D004195:Disease Models, Animal; D005780:Gelatin; D020100:Hydrogels; D007739:L Cells; D008422:Materials Testing; D008567:Membranes, Artificial; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010537:Peritoneum / Q000473:pathology; D011142:Polyvinyl Alcohol; D051381:Rats; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D000267:Tissue Adhesions / Q000517:prevention & control*
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D008567:Membranes, Artificial; D005780:Gelatin; D011142:Polyvinyl Alcohol
keywords: PVA; anti-adhesion; gelatin; peritoneal adhesion