title: Protective Effects of Vitamin C and NAC on the Toxicity of Rifampin on Hepg2 Cells.
abstract: Rifampin, an antibiotic widely used for the treatment of mycobacterial infections, produces hepatic, renal and bone marrow toxicity in human and animals. In this study, the protective effects of vitamin C and n-acetylcysteine (NAC) on the toxicity of rifampin on HepG2 cells were investigated. Human hepatoma cells (HepG2) were cultured in 96-well M of rifampin in the presence of microplate and exposed to 10, 20, 50 and 100 vitamin C (0.1 mg/mL) and NAC (0.2 mg/mL). Protective effect of the two drugs against rifampin toxicity was assessed by MTT assay. Results show that both vitamin C and NAC significantly inhibited HepG2 cellular damage due to rifampin, and vitamin C was relatively more potent than NAC. Rifampin is metabolized by the liver and its toxic metabolites are responsible for the drug›s hepatic toxicity. Based on our results, it seems that reactive metabolites are the main agents responsible for rifampin hepatotoxicity. The importance of this finding is that if vitamin C or NAC do not affect the antibacterial activity of rifampin, they could be used as preventive agents in rifampin users.
authors.affiliation: Department of Pharmacodynamy and Toxicology, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Nasser
authors.initials: N
authors.lastname: Vahdati-Mashhadian
authors.forename: Mahmoud Reza
authors.initials: MR
authors.lastname: Jafari
authors.forename: Nasim
authors.initials: N
authors.lastname: Sharghi
authors.forename: Toktam
authors.initials: T
authors.lastname: Sanati
keywords: Cell toxicity; HepG2; MTT assay; NAC; Vitamin C