title: Differential proteomic analysis of anthers between cytoplasmic male sterile and maintainer lines in Capsicum annuum L.
abstract: Cytoplasmic male sterility (CMS), widely used in the production of hybrid seeds, is a maternally inherited trait resulting in a failure to produce functional pollen. In order to identify some specific proteins associated with CMS in pepper, two-dimensional gel electrophoresis (2-DE) was applied to proteomic analysis of anthers/buds between a CMS line (designated NA3) and its maintainer (designated NB3) in Capsicum annuum L. Thirty-three spots showed more than 1.5-fold in either CMS or its maintainer. Based on mass spectrometry, 27 spots representing 23 distinct proteins in these 33 spots were identified. Proteins down-regulated in CMS anthers/buds includes ATP synthase D chain, formate dehydrogenase, alpha-mannosidas, RuBisCO large subunit-binding protein subunit beta, chloroplast manganese stabilizing protein-II, glutathione S-transferase, adenosine kinase isoform 1T-like protein, putative DNA repair protein RAD23-4, putative caffeoyl-CoA 3-O-methyltransferase, glutamine synthetase (GS), annexin Cap32, glutelin, allene oxide cyclase, etc. In CMS anthers/buds, polyphenol oxidase, ATP synthase subunit beta, and actin are up-regulated. It was predicted that male sterility in NA3 might be related to energy metabolism turbulence, excessive ethylene synthesis, and suffocation of starch synthesis. The present study lays a foundation for future investigations of gene functions associated with pollen development and cytoplasmic male sterility, and explores the molecular mechanism of CMS in pepper.
authors.affiliation: College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Zhongkai Road 501, Guangzhou 510225, Guangdong, China. hukailin@scau.edu.cn.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Wu
authors.forename: Jiaowen
authors.initials: J
authors.lastname: Cheng
authors.forename: Cheng
authors.initials: C
authors.lastname: Qin
authors.forename: Zhiqun
authors.initials: Z
authors.lastname: Hu
authors.forename: Caixia
authors.initials: C
authors.lastname: Yin
authors.forename: Kailin
authors.initials: K
authors.lastname: Hu
mesh_terms: D002212:Capsicum / Q000235:genetics / Q000254:growth & development*; D003593:Cytoplasm / Q000235:genetics / Q000473:pathology / Q000502:physiology; D015180:Electrophoresis, Gel, Two-Dimensional; D035264:Flowers / Q000235:genetics / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D013058:Mass Spectrometry; D051479:Plant Infertility / Q000235:genetics*; D010940:Plant Proteins / Q000096:biosynthesis; D011058:Pollen / Q000235:genetics* / Q000254:growth & development; D020543:Proteome / Q000032:analysis*
chemical_list: D010940:Plant Proteins; D020543:Proteome