title: Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens.
abstract: The leaf disc transformation/regeneration system was modified for tomato (L. esculentum). Both leaf explants and cotyledon/hypocotyl sections can be used to regenerate transformed plants. We have obtained over 300 transgenic plants from eight tomato cultivars. We have evidence for both single and multi-copy insertions of the T-DNA, and have demonstrated inheritance of the T-DNA insert in the expected Mendelian ratios. Several heterologous promoters function in tomato. A reduced efficiency of transformation was observed with binary T-DNA vectors as compared to co-integrate T-DNA vectors. The ease of the leaf disc method makes tomato a premier experimental organism for plant biotechnology.
authors.affiliation: Biological Sciences, Monsanto Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO, USA.
authors.forename: S
authors.initials: S
authors.lastname: McCormick
authors.forename: J
authors.initials: J
authors.lastname: Niedermeyer
authors.forename: J
authors.initials: J
authors.lastname: Fry
authors.forename: A
authors.initials: A
authors.lastname: Barnason
authors.forename: R
authors.initials: R
authors.lastname: Horsch
authors.forename: R
authors.initials: R
authors.lastname: Fraley