title: Tussilagone suppresses colon cancer cell proliferation by promoting the degradation of β-catenin.
abstract: Abnormal activation of the Wnt/β-catenin signaling pathway frequently induces colon cancer progression. In the present study, we identified tussilagone (TSL), a compound isolated from the flower buds of Tussilago farfara, as an inhibitor on β-catenin dependent Wnt pathway. TSL suppressed β-catenin/T-cell factor transcriptional activity and down-regulated β-catenin level both in cytoplasm and nuclei of HEK293 reporter cells when they were stimulated by Wnt3a or activated by an inhibitor of glycogen synthase kinase-3β. Since the mRNA level was not changed by TSL, proteasomal degradation might be responsible for the decreased level of β-catenin. In SW480 and HCT116 colon cancer cell lines, TSL suppressed the β-catenin activity and also decreased the expression of cyclin D1 and c-myc, representative target genes of the Wnt/β-catenin signaling pathway, and consequently inhibited the proliferation of colon cancer cells. Taken together, TSL might be a potential chemotherapeutic agent for the prevention and treatment of human colon cancer.
authors.affiliation: College of Pharmacy and Research Center for Cell Fate Control, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul 140-742, Republic of Korea.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Natural Medicine Resources, Semyung University, 65 Semyung-ro, Jecheon, Chungbuk 390-711, Republic of Korea.
authors.forename: Hwa Jin
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: College of Pharmacy and Research Center for Cell Fate Control, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul 140-742, Republic of Korea.
authors.forename: Yeon Hwa
authors.initials: YH
authors.lastname: Ahn
authors.affiliation: College of Pharmacy and Research Center for Cell Fate Control, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul 140-742, Republic of Korea.
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Kwon
authors.affiliation: College of Pharmacy and Research Center for Cell Fate Control, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul 140-742, Republic of Korea.
authors.forename: Chang-Young
authors.initials: CY
authors.lastname: Jang
authors.affiliation: College of Pharmacy and Research Center for Cell Fate Control, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul 140-742, Republic of Korea.
authors.forename: Woo-Young
authors.initials: WY
authors.lastname: Kim
authors.affiliation: College of Pharmacy and Research Center for Cell Fate Control, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul 140-742, Republic of Korea. Electronic address: ryuha@sookmyung.ac.kr.
authors.forename: Jae-Ha
authors.initials: JH
authors.lastname: Ryu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D057809:HEK293 Cells; D006801:Humans; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D059748:Proteolysis / Q000187:drug effects; D012717:Sesquiterpenes / Q000494:pharmacology*; D051153:Wnt Proteins / Q000037:antagonists & inhibitors; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D012717:Sesquiterpenes; D051153:Wnt Proteins; D051176:beta Catenin; C054337:tussilagone
keywords: Colon cancer; Tussilagone; β-Catenin