title: Carbon monoxide sensitivity of cytochrome oxidase in wheat leaves in relation to dwarfing genes (Rht) in wheat cultivars.
abstract: Leaves of young seedlings of a number of tall cultivars of wheat, lacking the dwarfing Rht genes, readily responded to a brief 2 min exposure to CO, as assessed by in vivo aerobic assay of nitrate reductase. This test depends on the inhibition of cytochrome c oxidase by CO, which in turn renders cytosolic NADH available for the reduction of nitrate to nitrite in vivo. Semi-dwarf cultivars of wheat (Rht present) did not respond to CO in this way. Since CO forms a complex only with reduced cytochrome a3, the results indicate differences in the redox state of cytochrome a3, during in situ respiration of leaves from tall and semi-dwarf plants which are likely to be under genetic control.
authors.affiliation: Department of Agricultural Biochemistry, Waite Agricultural Research Institute, University of Adelaide, 5064, Glen Osmond, South Australia.
authors.forename: M S
authors.initials: MS
authors.lastname: Naik
authors.forename: D J
authors.initials: DJ
authors.lastname: Nicholas