title: Synthesis, characterization and the interaction of some new water-soluble metal Schiff base complexes with human serum albumin.
abstract: Some new water-soluble Schiff base complexes of Na2[M(L)(H2O)n]; (M=Zn, Cu, Ni, Mn) with a new water-soluble Schiff base ligand where L denotes an asymmetric N2O2 Schiff base ligands; N,N'-bis(5-sulfosalicyliden)-3,4-diaminobenzophenone (5-SO3-3,4-salbenz) were synthesized and characterized. The formation constants of the water soluble Schiff base complexes were calculated by Ketelaar's equation. The theoretical molecular structure for the complexes was computed by using the HF method and the 6-311G basis set. The mechanism of binding of Na2[M(L)(H2O)n] with human serum albumin (HSA) was studied by fluorescence spectroscopic technique. The results of fluorescence titration showed that the intrinsic fluorescence of HSA was quenched by the complexes; which was rationalized in terms of the dynamic quenching mechanism. The values of Stern-Volmer constants, quenching rate constants, binding constants, binding sites and average aggregation number of HSA have been determined. The thermodynamic parameters, were calculated by van't Hoff equation, indicate that the binding is entropy driven and enthalpically disfavored. Based on the Förster theory of non-radiation energy transfer, the efficiency of energy transfer and the distance between the donor (Trp residues) and the acceptor (complex) were obtained. Finally, the growth inhibitory effects of the complexes toward the K562 cancer cell line were measured.
authors.affiliation: Chemistry Department, College of Sciences, Shiraz University, Shiraz 71454, Islamic Republic of Iran. Electronic address: asadi@susc.ac.ir.
authors.forename: Mozaffar
authors.initials: M
authors.lastname: Asadi
authors.affiliation: Chemistry Department, College of Sciences, Shiraz University, Shiraz 71454, Islamic Republic of Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Asadi
authors.affiliation: Chemistry Department, College of Sciences, Shiraz University, Shiraz 71454, Islamic Republic of Iran.
authors.forename: Somaye Barzegar
authors.initials: SB
authors.lastname: Sadi
authors.affiliation: Chemistry Department, College of Sciences, Shiraz University, Shiraz 71454, Islamic Republic of Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Zarei
authors.affiliation: Department of Chemistry, Ferdowsi University of Mashhad, Mashhad 91779, Islamic Republic of Iran.
authors.forename: Fatemeh Moosavi
authors.initials: FM
authors.lastname: Baigi
authors.affiliation: Immunology Department, Shiraz University of Medical Sciences, Shiraz 71454, Islamic Republic of Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Amirghofran
mesh_terms: D001665:Binding Sites; D049109:Cell Proliferation / Q000187:drug effects; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D020014:K562 Cells; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D010653:Phenylbutazone / Q000737:chemistry; D012545:Schiff Bases / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics; D013818:Thermogravimetry; D014867:Water / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D012545:Schiff Bases; D012709:Serum Albumin; D014867:Water; D010653:Phenylbutazone
keywords: Fluorescence; Human serum albumin; Schiff base