title: [Relationships of five periodontal pathogens causing subgingival plaque in patients with chronic periodontitis under different periodontal conditions].
abstract: OBJECTIVE
  This study aimed to assess the relationship between the distributions of Haemophilus aggregatibacter (H. aggregatibacter ), Porphyromonas gingivalis (P. gingivalis ), Prevotella intermedia (P. intermedia), Tannerella forsythensis (T. forsythensis), and Treponema denticola (T. denticola) in subgingival plaque and different periodontal conditions of chronic periodontitis.
  
  
  METHODS
  Twenty patients (80 sites) with chronic periodontitis and ten healthy subjects (20 sites) were included. The study sites were distributed into different groups according to the differences in their pocket probing depths (PD). The groups were described as follows: group A, PD< or = 4 mm; group B, 4 mm < PD < or = 6 mm; group C, PD>6 mm. Semi-quantification of the subgingival microorganism samples was analyzed by polymerase chain reaction (PCR) and reverse hybridization assay.
  
  
  RESULTS
  The prevalence rates and quantities of P. gingivalis, P. intermedia, T. forsythensis, and T. denticola were significantly higher in groups B and C than in the healthy group. Higher prevalence rates and quantities of P. gingivalis were detected in group A than in the healthy group. The quantities of T. forsythensis and T. denticola were also significantly higher in group C than in group B. However, the prevalence rates and quantities ofH. aggregatibacter showed no significant difference among the groups.
  
  
  CONCLUSIONS
  The prevalence rates and levels ofP. gingivalis, P. intermedia, T. forsythensis, and T. denticola possibly increased as the depths of the periodontal pockets increased. The quantity ofP. gingivalis was correlated with the early stage of chronic periodontitis. The quantities of T. forsythensis and T. denticola were associated with local development of moderate or severe chronic periodontitis.
authors.affiliation: Dept. of Stomatology, The People's Hospital of Guizhou Province, Guiyang 550002, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhou
authors.forename: Hong
authors.initials: H
authors.lastname: Xie
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Yue
mesh_terms: D001439:Bacteroides; D055113:Chronic Periodontitis; D003773:Dental Plaque; D006801:Humans; D010514:Periodontal Pocket; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis; D018713:Prevotella intermedia; D048373:Treponema denticola