title: Possible diagnostic role of antibodies to Crohn's disease peptide (ACP): results of a multicenter study in a Japanese cohort.
abstract: BACKGROUND
  Various noninvasive tests have been studied to screen for patients with Crohn's disease (CD), and were found to have limited accuracy and sensitivity, particularly in Asian populations. The aim of our study was to explore the possible diagnostic utility of antibodies to the CD peptide (ACP) in patients with CD.
  
  
  METHODS
  In a multicenter study using enzyme-linked immunosorbent assay, serum ACP levels were determined in 196 patients with CD, 210 with ulcerative colitis, 98 with other intestinal diseases, 132 with other inflammatory diseases, and 183 healthy controls. and then examined for correlation to clinical variables. The diagnostic utility of ACP was evaluated by receiver operating characteristics analysis and compared with anti-Saccharomyces cerevisiae antibodies (ASCA).
  
  
  RESULTS
  ACP levels were significantly elevated in the CD patients, but not in the other groups that included UC, other intestinal diseases, other inflammatory diseases and the healthy controls. Among these other groups, ACP levels were not significantly different. In the CD patients, ACP had a higher sensitivity and specificity (63.3 and 91.0 %, respectively) than ASCA (47.4 and 90.4 %). ACP levels were negatively associated with disease duration, but not with CDAI, disease location, or medical treatment.
  
  
  CONCLUSIONS
  ACP, a newly proposed serologic marker, was significantly associated with CD and was highly diagnostic. Further investigation is needed across multiple populations of patients and ethnic groups, and more importantly, in prospective studies.
authors.affiliation: Inflammatory Bowel Disease Center, Division of Gastroenterology, Department of Medicine, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan, ibd@med.kurume-u.ac.jp.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Mitsuyama
authors.forename: Mikio
authors.initials: M
authors.lastname: Niwa
authors.forename: Junya
authors.initials: J
authors.lastname: Masuda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamasaki
authors.forename: Kotaro
authors.initials: K
authors.lastname: Kuwaki
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Takedatsu
authors.forename: Teppei
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Fukunori
authors.initials: F
authors.lastname: Kinjo
authors.forename: Kazuto
authors.initials: K
authors.lastname: Kishimoto
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Matsui
authors.forename: Fumihito
authors.initials: F
authors.lastname: Hirai
authors.forename: Kazuya
authors.initials: K
authors.lastname: Makiyama
authors.forename: Kazuo
authors.initials: K
authors.lastname: Ohba
authors.forename: Hiroo
authors.initials: H
authors.lastname: Abe
authors.forename: Hirohito
authors.initials: H
authors.lastname: Tsubouchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fujita
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Maekawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Michio
authors.initials: M
authors.lastname: Sata
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D019540:Area Under Curve; D044466:Asian People; D003093:Colitis, Ulcerative / Q000097:blood / Q000175:diagnosis*; D003106:Colon; D003424:Crohn Disease / Q000097:blood / Q000175:diagnosis*; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007082:Ileum; D007074:Immunoglobulin G / Q000097:blood*; D007564:Japan; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000276:immunology*; D012372:ROC Curve; D012441:Saccharomyces cerevisiae / Q000276:immunology; D012720:Severity of Illness Index; D013997:Time Factors; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D010455:Peptides; C000594642:TCP-353 peptide