title: Transgene delivery to cultured keratinocytes via replication-deficient adenovirus vectors.
abstract: Transient transgene expression can facilitate investigation of that gene-product function or effect on keratinocyte biology. Several chemical and biologic delivery systems are available, and among them adenoviruses offer particular advantages in efficiency and transgene capacity. Here we describe the advantages of bicistronic adenovirus and inclusion of the polycation hexadimethrine bromide to aid in the detection of positively transduced cells and enhance transduction efficiency.
authors.affiliation: Graduate Program in Pharmacology & Toxicology, Department of Pharmaceutical Sciences, School of Pharmacy, University of Connecticut, Storrs, CT, USA.
authors.forename: Vincent P
authors.initials: VP
authors.lastname: Ramirez
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Aneskievich
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D002460:Cell Line; D003362:Cost-Benefit Analysis; D003470:Culture Media; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D006583:Hexadimethrine Bromide / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism*; D014161:Transduction, Genetic / Q000191:economics / Q000379:methods*; D019076:Transgenes / Q000235:genetics*
chemical_list: D003470:Culture Media; D006583:Hexadimethrine Bromide