title: Bioactivity and biocompatibility of a chitosan-tobermorite composite membrane for guided tissue regeneration.
abstract: A polymer-mineral composite membrane of the mucopolysaccharide derivative, chitosan, and calcium silicate hydrate phase, tobermorite, was prepared by solvent casting and characterised by scanning electron microscopy (SEM) and Fourier Transform infrared spectroscopy (FTIR). The bioactivity and biocompatibility of the chitosan-tobermorite composite were evaluated in vitro with respect to its potential for use as a biodegradable guided tissue regeneration (GTR) membrane. The in vitro bioactivity of the composite was confirmed by the formation of crystalline substituted hydroxyapatite on the surface of the embedded tobermorite particles in simulated body fluid. The presence of the composite membrane was found to enhance the growth of MG63 human osteosarcoma cells by up to 30%. The findings of this initial study have indicated that this novel chitosan-tobermorite composite may be a suitable material for GTR applications.
authors.affiliation: School of Science, University of Greenwich, Chatham Maritime, Kent ME4 4TB, UK.
authors.forename: A P
authors.initials: AP
authors.lastname: Hurt
authors.affiliation: School of Science, University of Greenwich, Chatham Maritime, Kent ME4 4TB, UK.
authors.forename: G
authors.initials: G
authors.lastname: Getti
authors.affiliation: School of Science, University of Greenwich, Chatham Maritime, Kent ME4 4TB, UK. Electronic address: nj_coleman@yahoo.co.uk.
authors.forename: N J
authors.initials: NJ
authors.lastname: Coleman
mesh_terms: D001672:Biocompatible Materials; D017610:Calcium Compounds / Q000737:chemistry*; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D048091:Guided Tissue Regeneration; D006801:Humans; D008422:Materials Testing; D008567:Membranes, Artificial; D017640:Silicates / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D017610:Calcium Compounds; D008567:Membranes, Artificial; D017640:Silicates; C512534:tobermorite; D048271:Chitosan
keywords: Bioactive; Chitosan; Tobermorite