title: Phosphoglycerate kinase 1 promotes radioresistance in U251 human glioma cells.
abstract: Phosphoglycerate kinase 1 (PGK1) has been found to be increased in radioresistant astrocytomas. The present study was designed to investigate the potential role of PGK1 in the radioresistance in U251 human cells. Quantitative PCR and western blot analysis were performed to evaluate PGK1 expression for mRNA levels and protein levels, respectively. The short hairpin RNA (shRNA)-PGK1 and the high expression plasmids were transfected to radioresistant U251 cells (RR-U251 cells) or normal U251 cells using lipofectamine™ 2000. The cell viability was determined by MTT assay. The wound-healing assay (WHA) was used to evaluate cell migration ability. Cell invasion abilities were examined using a Transwell culture chamber system. Our results showed that the expression of PGK1 was significantly increased in RR-U251 cells compared to normal U251 cells. Following irradiation, the cell viability as well as the migration and invasion ability were significantly higher in RR-U251 cells compared with normal U251 cells. Downregulating PGK1 using shRNA induced a significantly downregulated cell viability and decreased migration and invasion ability, and overexpression of PGK1 contributed to upregulated cell viability and increased migration and invasion ability, both in RR-U251 cells and normal U251 cells. These findings suggest that PGK1 could promote radioresistance in U251 human cells.
authors.affiliation: Department of Neurosurgery, Nanjing Medical University, Affiliated Nanjing Brain Hospital, Nanjing, Jiangsu, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Ding
authors.affiliation: Department of Neurosurgery, Nanjing Medical University, Affiliated Nanjing Brain Hospital, Nanjing, Jiangsu, P.R. China.
authors.forename: Yi-Jun
authors.initials: YJ
authors.lastname: Cheng
authors.affiliation: Department of Neurosurgery, Nanjing Medical University, Affiliated Nanjing Brain Hospital, Nanjing, Jiangsu, P.R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yan
authors.affiliation: Department of Neurosurgery, Nanjing Medical University, Affiliated Nanjing Brain Hospital, Nanjing, Jiangsu, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Nanjing Medical University, Affiliated Nanjing Brain Hospital, Nanjing, Jiangsu, P.R. China.
authors.forename: Jin-Bing
authors.initials: JB
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, Nanjing Medical University, Affiliated Nanjing Brain Hospital, Nanjing, Jiangsu, P.R. China.
authors.forename: Chun-Fa
authors.initials: CF
authors.lastname: Qian
authors.affiliation: Department of Neurosurgery, Nanjing Medical University, Affiliated Nanjing Brain Hospital, Nanjing, Jiangsu, P.R. China.
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Zhang
authors.affiliation: Neuro-Psychiatric Institute, Nanjing Medical University, Affiliated Nanjing Brain Hospital, Nanjing, Jiangsu, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Department of Neurosurgery, Nanjing Medical University, Affiliated Nanjing Brain Hospital, Nanjing, Jiangsu, P.R. China.
authors.forename: Hong-Yi
authors.initials: HY
authors.lastname: Liu
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics / Q000532:radiotherapy*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002470:Cell Survival / Q000528:radiation effects; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics / Q000532:radiotherapy*; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics; D010735:Phosphoglycerate Kinase / Q000096:biosynthesis / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering; D011836:Radiation Tolerance / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; C577282:PGK1 protein, human; D010735:Phosphoglycerate Kinase