title: Amentoflavone inhibits angiogenesis of endothelial cells and stimulates apoptosis in hypertrophic scar fibroblasts.
abstract: Amentoflavone (8-[5-(5,7-dihydroxy-4-oxo-chromen-2-yl)-2-hydroxy-phenyl]-5,7-dihydroxy-2-(4-hydroxyphenyl) chromen-4-one; AF) is a biflavonoid derived from the extracts of Selaginella tamariscina. It has been shown that AF has diverse biological effects such as antitumour, etc. It is well known that high cell proliferation, viability, angiogenesis and low apoptosis are key factors in hypertrophic scar formation. In this study, we report that AF inhibited viability and stimulated apoptosis in hypertrophic scar fibroblasts (HSFBs). Incubation of HSFBs with AF showed its inhibitory effect on cell viability and the exhibition of a series of cellular changes that were consistent with apoptosis. By Western-blot analysis, our data indicated significant increases in the amounts of cleaved caspases 3, 8, 9 and Bax, several apoptotic promoters and a significant decrease in translationally controlled tumour protein (TCTP), an apoptotic inhibitor, in HSFBs treated with AF. Furthermore, AF showed significant inhibitions on the viability, migration and tube formation of endothelial cells, which are associated with angiogenesis. In conclusion, this study suggests that AF stimulates apoptosis in HSFBs and inhibits angiogenesis of endothelial cells. Therefore, AF is a promising molecule that can be used in hypertrophic scar treatment.
authors.affiliation: Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, Guangdong, China.
authors.forename: Jinli
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, Guangdong, China. Electronic address: zliu0731@163.com.
authors.forename: Zhihe
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, Guangdong, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Cao
authors.affiliation: Department of Burn and Plastic Surgery, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, Guangdong, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Chen
authors.affiliation: Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, Guangdong, China.
authors.forename: Xifeng
authors.initials: X
authors.lastname: Xiong
authors.affiliation: Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, Guangdong, China.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Qin
authors.affiliation: Department of Burn and Plastic Surgery, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, Guangdong, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Burn and Plastic Surgery, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, Guangdong, China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, University of New Mexico School of Medicine, Albuquerque, NM, USA.
authors.forename: Chien-an A
authors.initials: CA
authors.lastname: Hu
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D044946:Biflavonoids / Q000494:pharmacology*; D014408:Biomarkers, Tumor; D020169:Caspases / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D017439:Cicatrix, Hypertrophic; D042783:Endothelial Cells / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D000090324:Tumor Protein, Translationally-Controlled 1
chemical_list: D020533:Angiogenesis Inhibitors; D044946:Biflavonoids; D014408:Biomarkers, Tumor; C000715647:TPT1 protein, human; C000715648:Tpt1 protein, mouse; D000090324:Tumor Protein, Translationally-Controlled 1; C011164:amentoflavone; D020169:Caspases
keywords: Amentoflavone (AF); Angiogenesis; Apoptosis; Endothelial cells (ECs); Hypertrophic scar fibroblasts (HSFBs)