title: Allergenicity and toxicology of inhaled silver nanoparticles in allergen-provocation mice models.
abstract: Silver nanoparticles (AgNP) have been associated with the exacerbation of airway hyperresponsiveness. However, the allergenicity and toxicology of AgNP in healthy and allergic individuals are unclear. We investigated the pathophysiological responses to AgNP inhalation in a murine model of asthma. Continuous and stable levels of 33 nm AgNP were maintained at 3.3 mg/m(3) during the experimental period. AgNP exposure concomitant with ovalbumin challenge increased the enhanced pause (Penh) in the control and allergic groups. AgNP evoked neutrophil, lymphocyte and eosinophil infiltration into the airways and elevated the levels of allergic markers (immunoglobulin E [IgE] and leukotriene E4 [LTE4]), the type 2 T helper (Th2) cytokine interleukin-13 (IL-13), and oxidative stress (8-hydroxy-2'-deoxyguanosine [8-OHdG]) in healthy and allergic mice. Bronchocentric interstitial inflammation was observed after AgNP inhalation. After inhalation, the AgNP accumulated predominantly in the lungs, and trivial amounts of AgNP were excreted in the urine and feces. Furthermore, the AgNP induced inflammatory responses in the peritoneum. The inhalation of AgNP may present safety concerns in healthy and susceptible individuals.
authors.affiliation: School of respiratory Therapy, College of Medicine Shuang Ho Hospital, Taipei Medical University, Taipei, Taiwan ; Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, Taipei, Taiwan.
authors.forename: Hsiao-Chi
authors.initials: HC
authors.lastname: Chuang
authors.forename: Ta-Chih
authors.initials: TC
authors.lastname: Hsiao
authors.forename: Cheng-Kuan
authors.initials: CK
authors.lastname: Wu
authors.forename: Hui-Hsien
authors.initials: HH
authors.lastname: Chang
authors.forename: Chii-Hong
authors.initials: CH
authors.lastname: Lee
authors.forename: Chih-Cheng
authors.initials: CC
authors.lastname: Chang
authors.forename: Tsun-Jen
authors.initials: TJ
authors.lastname: Cheng
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000280:Administration, Inhalation; D000818:Animals; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D005260:Female; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D009928:Organ Specificity; D010047:Ovalbumin; D018384:Oxidative Stress / Q000187:drug effects*; D011014:Pneumonia / Q000139:chemically induced* / Q000276:immunology / Q000473:pathology; D012130:Respiratory Hypersensitivity / Q000276:immunology; D012834:Silver / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology* / Q000633:toxicity*
chemical_list: D012834:Silver; D000080242:8-Hydroxy-2'-Deoxyguanosine; D010047:Ovalbumin; D003849:Deoxyguanosine
keywords: inductively coupled plasma-mass spectrometry; inflammation; leukotriene E4; ovalbumin; oxidative stress