title: Hepatic transcriptome profiling identifies differences in expression of genes associated with changes in metabolism and postnatal growth between Hereford and Holstein-Friesian bulls.
abstract: This study examined liver transcriptomic profiles of cattle distinctly different in meat and milk production capacity. It was performed on bulls of two different genetic backgrounds: Herefords (H), a meat breed, and Holstein-Friesians (HF), a dairy breed. Using bovine long oligo-microarrays and qPCR, we identified 128 genes that are differentially expressed between the two breeds. In H bulls, we observed up-regulation of genes involved in fatty acid biosynthesis and lipid metabolism (CD36, CAT, HSD3B1, FABP1, ACAA1) and involved in insulin signaling (INSR, INSIG2, NR4A1) and down-regulation of genes involved in somatotropic axis signaling (IGF1, GHR, IGFBP3) as compared to HF. Transcriptome profiling of these two breeds allowed us to pinpoint the transcriptional differences between Holstein and Hereford bulls at hepatic level associated with changes in metabolism and postnatal growth.
authors.affiliation: Department of Molecular Biology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Postepu 36A, 05-552, Magdalenka, Jastrzebiec n/Warsaw, Poland.
authors.forename: Pawel
authors.initials: P
authors.lastname: Lisowski
authors.forename: Ewa M
authors.initials: EM
authors.lastname: Kościuczuk
authors.forename: Joanna
authors.initials: J
authors.lastname: Gościk
authors.forename: Mariusz
authors.initials: M
authors.lastname: Pierzchała
authors.forename: Barbara
authors.initials: B
authors.lastname: Rowińska
authors.forename: Lech
authors.initials: L
authors.lastname: Zwierzchowski
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D020869:Gene Expression Profiling; D008099:Liver / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D059467:Transcriptome
chemical_list: D012333:RNA, Messenger
keywords: cattle; fatty acid/lipid metabolism mechanisms; gene expression; growth hormone; insulin-like growth factor; liver; microarray; qPCR; somatotropic axis; transcriptomics