title: Platelet-derived growth-factor-releasing aligned collagen-nanoparticle fibers promote the proliferation and tenogenic differentiation of adipose-derived stem cells.
abstract: In order to enhance the healing potential of an injured tendon, we have prepared a novel biomimetic aligned collagen-nanoparticle (NP) composite fiber using an electrochemical process. The aligned collagen-NP composite fiber is designed to affect the cellular activity of adipose-derived stem cells (ADSCs) through two different ways: (i) topographic cues from the alignment of collagen fibril and (ii) controlled release of platelet-derived growth factors (PDGFs) from the NPs. PDGF released from collagen-NP fibers significantly enhanced the proliferation of ADSCs when tested for up to 7 days. Moreover, compared to random collagen fibers with PDGFs, aligned collagen-NP fibers significantly promoted the desirable tenogenic differentiation of ADSCs, as evidenced by an increased level of tendon markers such as tenomodulin and scleraxis. On the other hand, no undesirable osteogenic differentiation, as measured by the unchanged level of alkaline phosphatase and osteocalcin, was observed. Together, these results indicate that the aligned collagen-NP composite fiber induced the tenogenic differentiation of ADSCs through both a topographic cue (aligned collagen fibril) and a chemical cue (PDGF released from NPs). Thus, our novel aligned collagen-NP composite fiber has a significant potential to be used for tendon tissue engineering and regeneration.
authors.affiliation: Microencapsulation and Nanomaterials Department, Southwest Research Institute, 6220 Culebra Rd, San Antonio, TX 78238, USA. Electronic address: xcheng@swri.org.
authors.forename: XingGuo
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Microencapsulation and Nanomaterials Department, Southwest Research Institute, 6220 Culebra Rd, San Antonio, TX 78238, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Tsao
authors.affiliation: Department of Orthopedics, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX 78229, USA.
authors.forename: Victor L
authors.initials: VL
authors.lastname: Sylvia
authors.affiliation: Department of Orthopedics, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX 78229, USA.
authors.forename: Douglas
authors.initials: D
authors.lastname: Cornet
authors.affiliation: Materials Engineering Department, Southwest Research Institute, 6220 Culebra Rd, San Antonio, TX 78238, USA.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Nicolella
authors.affiliation: Materials Engineering Department, Southwest Research Institute, 6220 Culebra Rd, San Antonio, TX 78238, USA.
authors.forename: Todd L
authors.initials: TL
authors.lastname: Bredbenner
authors.affiliation: Extremity Trauma and Regenerative Medicine Program, US Army Institute of Surgical Research, 3698 Chambers Pass, Fort Sam Houston, TX 78234, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Christy
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001696:Biomechanical Phenomena / Q000187:drug effects; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D055664:Electrochemical Techniques; D008297:Male; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D011091:Polyesters / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011917:Rats, Inbred Lew; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013710:Tendons / Q000166:cytology* / Q000187:drug effects
chemical_list: D010982:Platelet-Derived Growth Factor; D011091:Polyesters; C558447:methoxypolyethyleneglycol-poly(lactic-co-glycolic acid); D011092:Polyethylene Glycols; D003094:Collagen
keywords: Collagen; Growth Factors; Nanoparticle; Stem cell; Tendon