title: Anthocyanidins, proanthocyanidins, and anthocyanins profiling in wine lees by solid-phase extraction-liquid chromatography coupled to electrospray ionization tandem mass spectrometry with data-dependent methods.
abstract: A method has been developed to study the content of anthocyanidins, proanthocyanidicins, and anthocyanins in wine lees, an abundant byproduct from wineries. Detection/quantitation of the target compounds was carried out by a hyphenated system consisting of a solid-phase extraction workstation (Prospekt-2 unit) online coupled to a liquid chromatograph-triple-quadrupole tandem mass spectrometer (LC-MS/MS), where standards were used for identification/quantitation of both anthocyanidins and proanthocyanidins. Owing to the lack of anthocyanins standards, advantages from the use of data-dependent methods were taken for their identification and confirmatory analysis. Combination of the scanning methods (viz. product-ion, precursor-ion, and neutral-loss scanning) allowed identifying five different anthocyanins present in wine residues. The results thus obtained have been validated by complementary analysis of the extracts using LC-TOF/MS in high-resolution mode. Quantitation of the monitored compounds was supported on selected reaction monitoring (SRM) and calibration curves run with standards of anthocyanidins and proanthocyanidins.
authors.affiliation: Department of Analytical Chemistry, University of Córdoba , Annex Marie Curie Building, Campus of Rabanales, 14071 Córdoba, Spain.
authors.forename: M P
authors.initials: MP
authors.lastname: Delgado de la Torre
authors.forename: C
authors.initials: C
authors.lastname: Ferreiro-Vera
authors.forename: F
authors.initials: F
authors.lastname: Priego-Capote
authors.forename: M D
authors.initials: MD
authors.lastname: Luque de Castro
mesh_terms: D000872:Anthocyanins / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D044945:Proanthocyanidins / Q000737:chemistry* / Q000302:isolation & purification; D052616:Solid Phase Extraction / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods; D014920:Wine / Q000032:analysis*
chemical_list: D000872:Anthocyanins; D044945:Proanthocyanidins