title: Effect of BMP-2 protein on the count and osteogenic properties of multipotent stromal cells and expression of cytokine genes in primary cultures of bone marrow and spleen cells from CBA mice immunized with bacterial antigens.
abstract: We studied the effect of BMP-2 added to the culture medium on osteogenic and proliferative properties of multipotent stromal cells (MSC) and on the expression of cytokine genes induced by immunization of experimental animals with bacterial antigens. It is shown that the presence of BMP-2 in the culture medium stimulates proliferation of bone marrow MSC and especially spleen MSC (which was seen from enlargement of MSC colonies); improves the efficiency of MSC cloning; increases osteogenic activity of mouse bone marrow MSC; induces osteogenic differentiation of splenic MSC (osteogenesis is normally not observed in the spleen); reduces the number of macrophages in cultures; inhibits synthesis of mRNA for proinflammatory cytokines (IL-1β, IL-6, IL-8, TNF-α) that typically occurs in cultures of the bone marrow and spleen from animals immunized with S. typhimurium or group A streptococcus antigens. Bearing in mind that proinflammatory cytokines negatively affect osteogenic activity of the bone marrow, we can hypothesize that BMP-2 not only stimulates osteogenesis, but also provides optimal conditions for its realization by suppressing the expression of genes encoding these cytokines.
authors.affiliation: Laboratory of Immunity Regulation, Laboratory of Microbiology of Latent Infections, Laboratory of Bioactive Nanostructures, Laboratory of Stromal Regulation of Immunity, N. F. Gamaleya Institute of Epidemiology and Microbiology, Ministry of Health and Social Development, Moscow, Russia. Uliya.Gorskaya@nearmedic.ru.
authors.forename: Yu F
authors.initials: YF
authors.lastname: Gorskaya
authors.forename: T A
authors.initials: TA
authors.lastname: Danilova
authors.forename: M V
authors.initials: MV
authors.lastname: Mezentseva
authors.forename: I M
authors.initials: IM
authors.lastname: Shapoval
authors.forename: T M
authors.initials: TM
authors.lastname: Grunina
authors.forename: M S
authors.initials: MS
authors.lastname: Bartov
authors.forename: A S
authors.initials: AS
authors.lastname: Karyagina
authors.forename: V G
authors.initials: VG
authors.lastname: Lunin
authors.forename: R K
authors.initials: RK
authors.lastname: Chailakhyan
authors.forename: A I
authors.initials: AI
authors.lastname: Kuralesova
authors.forename: Yu V
authors.initials: YV
authors.lastname: Gerasimov
authors.forename: V G
authors.initials: VG
authors.lastname: Nesterenko
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D015870:Gene Expression; D007114:Immunization; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D016209:Interleukin-8 / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D010011:Osteocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D010012:Osteogenesis / Q000187:drug effects; D061251:Primary Cell Culture; D012333:RNA, Messenger / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D000093242:Interleukin-6 Inhibitors
chemical_list: D000942:Antigens, Bacterial; D055396:Bone Morphogenetic Protein 2; D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D000093242:Interleukin-6 Inhibitors; C525719:Bmp2 protein, mouse; C587672:IL1B protein, mouse