title: Palmitoylation and p8-mediated human T-cell leukemia virus type 1 transmission.
abstract: The orf-I gene of human T-cell leukemia type 1 (HTLV-1) encodes p8 and p12 and has a conserved cysteine at position 39. p8 and p12 form disulfide-linked dimers, and only the monomeric forms of p8 and p12 are palmitoylated. Mutation of cysteine 39 to alanine (C39A) abrogated dimerization and palmitoylation of both proteins. However, the ability of p8 to localize to the cell surface and to increase cell adhesion and viral transmission was not affected by the C39A mutation.
authors.affiliation: Animal Models and Retroviral Vaccines Section, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Dustin
authors.initials: D
authors.lastname: Edwards
authors.forename: Risaku
authors.initials: R
authors.lastname: Fukumoto
authors.forename: Maria Fernanda
authors.initials: MF
authors.lastname: de Castro-Amarante
authors.forename: Luiz Carlos Junior
authors.initials: LC
authors.lastname: Alcantara
authors.forename: Bernardo
authors.initials: B
authors.lastname: Galvão-Castro
authors.forename: Robyn
authors.initials: R
authors.lastname: Washington Parks
authors.forename: Cynthia
authors.initials: C
authors.lastname: Pise-Masison
authors.forename: Genoveffa
authors.initials: G
authors.lastname: Franchini
mesh_terms: D000595:Amino Acid Sequence; D017931:DNA Primers / Q000235:genetics; D019281:Dimerization; D057809:HEK293 Cells; D015490:HTLV-I Infections / Q000635:transmission*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics* / Q000502:physiology; D006801:Humans; D015151:Immunoblotting; D047468:Immunoprecipitation; D054878:Lipoylation / Q000502:physiology*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D020125:Mutation, Missense; D021122:Protein Subunits / Q000378:metabolism; D021381:Protein Transport; D054334:Viral Regulatory and Accessory Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D017931:DNA Primers; D021122:Protein Subunits; D054334:Viral Regulatory and Accessory Proteins; C084305:p12I protein, Human T-lymphotropic virus 1