title: Effect of the pro-resolution lipid mediator Resolvin E1 (RvE1) on pulp tissues exposed to the oral environment.
abstract: OBJECTIVE
  To evaluate the effects of topical Resolvin E1 (RvE1) application on infected dental pulps.
  
  
  METHODS
  Forty-two male Wistar rats (n = 6 per three groups/and two time periods) were used. To induce inflammation, pulps in mandibular right first molars were accessed and then left exposed to the oral environment for 24 h. After this period, topical medication with a corticosteroid/antibiotic blend, or RvE1, or its vehicle (Ethanol 0.1%) was directly applied onto the pulp tissue and teeth were restored with silver amalgam. The effects of the protocols were evaluated histologically and compared with control pulps not exposed to the oral environment. The inflammatory changes after 24 and 72 h were assessed through a scoring method and analysed using the Kruskal-Wallis test followed by Dunn's. Differences were considered significant if P < 0.05 (CI = 95%).
  
  
  RESULTS
  Ethanol and corticosteroid/antibiotic treatment were not effective in arresting severe inflammatory alterations of exposed pulps at 24 and 72 h (P < 0.05, CI = 95%). At both time periods, RvE1 treatment led to a reduction of tissue cellularity and extent of inflammation, whose changes were not different from control pulps (P > 0.05, CI = 95%).
  
  
  CONCLUSIONS
  A protective role for RvE1 in pulp inflammation was observed even in the presence of contamination, suggesting that it may be a candidate for a novel therapeutic strategy for conservative dental pulp treatment.
authors.affiliation: Pontificia Universidade Catolica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
authors.forename: L
authors.initials: L
authors.lastname: Dondoni
authors.forename: R K
authors.initials: RK
authors.lastname: Scarparo
authors.forename: A
authors.initials: A
authors.lastname: Kantarci
authors.forename: T E
authors.initials: TE
authors.lastname: Van Dyke
authors.forename: J A P
authors.initials: JA
authors.lastname: Figueiredo
authors.forename: E L
authors.initials: EL
authors.lastname: Batista
mesh_terms: D000305:Adrenal Cortex Hormones / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology; D003782:Dental Pulp / Q000187:drug effects* / Q000473:pathology; D015118:Eicosapentaenoic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000305:Adrenal Cortex Hormones; D000900:Anti-Bacterial Agents; D015118:Eicosapentaenoic Acid; C499823:5S,12R,18R-trihydroxy-6Z,8E,10E,14Z,16E-eicosapentaenoic acid
keywords: Resolvin E1; dental pulp; inflammation; resolution