title: Changes in cytoskeletal dynamics and nonlinear rheology with metastatic ability in cancer cell lines.
abstract: Metastatic outcome is impacted by the biophysical state of the primary tumor cell. To determine if changes in cancer cell biophysical properties facilitate metastasis, we quantified cytoskeletal biophysics in well-characterized human skin, bladder, prostate and kidney cell line pairs that differ in metastatic ability. Using magnetic twisting cytometry with optical detection, cytoskeletal dynamics was observed through spontaneous motion of surface bound marker beads and nonlinear rheology was characterized through large amplitude forced oscillations of probe beads. Measurements of cytoskeletal dynamics and nonlinear rheology differed between strongly and weakly metastatic cells. However, no set of biophysical parameters changed systematically with metastatic ability across all cell lines. Compared to their weakly metastatic counterparts, the strongly metastatic kidney cancer cells exhibited both increased cytoskeletal dynamics and stiffness at large deformation which are thought to facilitate the process of vascular invasion.
authors.forename: Mark F
authors.initials: MF
authors.lastname: Coughlin
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Fredberg
mesh_terms: D045744:Cell Line, Tumor; D003599:Cytoskeleton / Q000737:chemistry / Q000473:pathology*; D006801:Humans; D007668:Kidney / Q000737:chemistry / Q000166:cytology / Q000473:pathology; D008297:Male; D009362:Neoplasm Metastasis / Q000473:pathology*; D009369:Neoplasms / Q000737:chemistry / Q000473:pathology*; D011467:Prostate / Q000737:chemistry / Q000166:cytology / Q000473:pathology; D012212:Rheology; D012867:Skin / Q000737:chemistry / Q000166:cytology / Q000473:pathology; D001743:Urinary Bladder / Q000737:chemistry / Q000166:cytology / Q000473:pathology