title: P-gp expression in brown trout erythrocytes: evidence of a detoxification mechanism in fish erythrocytes.
abstract: Blood is a site of physiological transport for a great variety of molecules, including xenobiotics. Blood cells in aquatic vertebrates, such as fish, are directly exposed to aquatic pollution. P-gp are ubiquitous "membrane detoxification proteins" implicated in the cellular efflux of various xenobiotics, such as polycyclic aromatic hydrocarbons (PAHs), which may be pollutants. The existence of this P-gp detoxification system inducible by benzo [a] pyrene (BaP), a highly cytotoxic PAH, was investigated in the nucleated erythrocytes of brown trout. Western blot analysis showed the expression of a 140-kDa P-gp in trout erythrocytes. Primary cultures of erythrocytes exposed to increasing concentrations of BaP showed no evidence of cell toxicity. Yet, in the same BaP-treated erythrocytes, P-gp expression increased significantly in a dose-dependent manner. Brown trout P-gp erythrocytes act as membrane defence mechanism against the pollutant, a property that can be exploited for future biomarker development to monitor water quality.
authors.affiliation: 1] Clermont Université - Université d'Auvergne - ERTICa- EA 4677 - Institut Universitaire de Technologie, département génie biologique, Ensemble universitaire des Cézeaux, B.P. 86 - 63172 AUBIERE CEDEX France [2] Clermont Université - Université d'Auvergne - ERTICa - EA 4677- Centre Jean Perrin, 58 Rue Montalembert, BP 392 - 63011 CLERMONT-FERRAND CEDEX France [3] Clermont Université - Université Blaise Pascal - LMGE - UMR CNRS 6023 - BP 80026- 63171 AUBIERE CEDEX France.
authors.forename: Emeline
authors.initials: E
authors.lastname: Valton
authors.forename: Christian
authors.initials: C
authors.lastname: Amblard
authors.forename: Ivan
authors.initials: I
authors.lastname: Wawrzyniak
authors.forename: Frederique
authors.initials: F
authors.lastname: Penault-Llorca
authors.forename: Mahchid
authors.initials: M
authors.lastname: Bamdad
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism; D018929:Cell Culture Techniques; D004912:Erythrocytes / Q000378:metabolism*; D015870:Gene Expression; D008658:Inactivation, Metabolic; D014337:Trout / Q000235:genetics* / Q000378:metabolism*
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D001564:Benzo(a)pyrene