title: Osteogenic Differentiation of Bone Marrow Stem Cell in Poly(Lactic-co-Glycolic Acid) Scaffold Loaded Various Ratio of Hydroxyapatite.
abstract: OBJECTIVE
  Hydroxyapatite has biocompatibility and bioactivity and similar to bone of in human body. The purpose of this study is to evaluate osteogenic differentiation of bone marrow stem cell (BMSC) in PLGA Scaffold added various ratio of hydroxyapatite (HAp).
  
  
  RESULTS
  PLGA and PLGA/HAp scaffold were prepared using solvent casting/salt-leaching method. BMSC was seeded on the PLGA and PLGA/HAp scaffold and the samples were cultured in 37℃ incubator with 5% CO2 for 28 days. Alkaline phosphatase (ALP) was carried out to evaluate alkaline phosphatase activity at 1, 3, 7, 10 and 14 days. Alizarin Red S stating was performed to identify calcium in scaffold at 1, 7, 14, 21 and 28 days. Compressive strength was measured to evaluate mechanical property of scaffold. To confirm cell viability, MTT was carried out at 1, 3, 7, 14 and 28 days. RT-PCR was performed to verify specific marker expression of osteoblast and stem cell at 7, 14, 21 and 28 days.
  
  
  CONCLUSIONS
  Osteogenic differentiation of BMSC was confirmed through ALP, RT-PCR, and alizarin red S staining in this study. These results suggest that HAp helps osteogenic differentiation of BMSC.
authors.affiliation: Departments of BIN Fusion Technology ; Polymer-Nano Science & Technology, Chonbuk National University, Jeonju, Korea.
authors.forename: Hyeongseok
authors.initials: H
authors.lastname: Kim
authors.forename: Hye Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Jang
authors.forename: Cho Min
authors.initials: CM
authors.lastname: Kim
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Kim
authors.forename: Dongwon
authors.initials: D
authors.lastname: Lee
authors.forename: Gilson
authors.initials: G
authors.lastname: Khang
keywords: Bone marrow stem cell; Hydroxyapatite; Osteogenic differentiation; Poly(lactic-co-glycolic acid); Scaffold