title: Crowding alters the dynamics and the length of RecA nucleoprotein filaments in RecA-mediated strand exchange.
abstract: Crowd impact: Molecular crowding effects of bovine serum albumin and poly(ethylene glycol) on the Escherichia coli RecA-mediated strand exchange reaction are quantified by using a single-molecule outgoing strand experiment and magnetic pull-down and ATPase assays. The alterations of the biochemical parameters of this complex enzymatic reaction in such crowded environments are discussed.
authors.affiliation: Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C). Fax: (+886) 2-2363-6359.
authors.forename: Hung-Yi
authors.initials: HY
authors.lastname: Wu
authors.forename: Hung-Wen
authors.initials: HW
authors.lastname: Li
mesh_terms: D000818:Animals; D002417:Cattle; D004277:DNA, Single-Stranded / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009698:Nucleoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry*; D011938:Rec A Recombinases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D004277:DNA, Single-Stranded; D029968:Escherichia coli Proteins; D009698:Nucleoproteins; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D011938:Rec A Recombinases
keywords: DNA; dehydration; filaments; molecular crowding; strand exchange