title: Using a reverse genetics approach to investigate small-molecule activity.
abstract: Chemical genomics is a highly effective approach for understanding complex and dynamic biological processes in plants. A chemical activity can be investigated by a reverse genetics strategy, for which a huge abundance and diversity of Arabidopsis thaliana mutants are readily available for exploitation. Here we present an approach to characterize a chemical of interest, as well as examples of studies demonstrating an effective combination of chemical genomics with reverse genetics strategies, drawn from recent literature on phytohormone signalling and auxin transport.
authors.affiliation: Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences (SLU), Umeå, Sweden.
authors.forename: Siamsa M
authors.initials: SM
authors.lastname: Doyle
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Robert
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics*; D001692:Biological Transport; D004353:Drug Evaluation, Preclinical; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007210:Indoleacetic Acids / Q000378:metabolism; D009154:Mutation; D010937:Plant Growth Regulators / Q000494:pharmacology*; D059386:Reverse Genetics; D036226:Seedlings / Q000187:drug effects / Q000235:genetics; D015398:Signal Transduction
chemical_list: D007210:Indoleacetic Acids; D010937:Plant Growth Regulators