title: Application of Raman spectroscopy for visualizing biochemical changes during peripheral nerve injury in vitro and in vivo.
abstract: Raman spectroscopy can be used for analysis of objects by detecting the vibrational spectrum using label-free methods. This imaging method was applied to analysis of peripheral nerve regeneration by examining the sciatic nerve in vitro and in vivo. Raman spectra of intact nerve tissue had three particularly important peaks in the range 2800-3000 cm-1. Spectra of injured sciatic nerves showed significant changes in the ratio of these peaks. Analysis of cellular spectra suggested that the spectrum for sciatic nerve tissue reflects the axon and myelin components of this tissue. Immunohistochemical analysis showed that the number of axons and the myelinated area were reduced at 7 days after injury and then increased by 28 days. The relative change in the axon to myelin ratio showed a similar initial increase, followed by a decrease at 28 days after injury. These changes correlated with the band intensity ratio and the changes in distribution of axon and myelin in Raman spectral analysis. Thus, our results suggest that label-free biochemical imaging with Raman spectroscopy can be used to detect turnover of axon and myelin in peripheral nerve regeneration.
authors.affiliation: Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Department of Orthopaedics, Kawaramachi Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Morisaki
authors.forename: Chikashi
authors.initials: C
authors.lastname: Ota
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Matsuda
authors.forename: Natsuko
authors.initials: N
authors.lastname: Kaku
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Fujiwara
authors.forename: Ryo
authors.initials: R
authors.lastname: Oda
authors.forename: Hidenobu
authors.initials: H
authors.lastname: Ishibashi
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Kubo
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Kawata
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000737:chemistry / Q000166:cytology; D009186:Myelin Sheath / Q000378:metabolism; D059348:Peripheral Nerve Injuries / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000737:chemistry / Q000166:cytology; D012584:Sciatic Nerve / Q000737:chemistry* / Q000293:injuries* / Q000378:metabolism; D013059:Spectrum Analysis, Raman / Q000379:methods*