title: Development of a new parallelized, optical biosensor platform for label-free detection of autoimmunity-related antibodies.
abstract: Autoimmune diseases are characterized by the presence of autoantibodies in serum of affected patients. The heterogeneity of autoimmune relevant antigens creates a variety of different antibodies, which requires a simultaneous detection mode. For this reason, we developed a tool for parallelized, label-free, optical detection that accomplishes the characterization of multiple antigen-antibody interactions within a single measurement on a timescale of minutes. Using 11-aminoundecyltrimethoxysilane, we were able to immobilize proteinogenic antigens as well as an amino-functionalized cardiolipin on a glass surface. Assay conditions were optimized for serum measurements with a single spot antigen chip on a single spot 1-λ detection system. Minimized background signal allows a differentiation between patients and healthy controls with a good sensitivity and specificity. Applying polarized imaging reflectometric interference spectroscopy, we evaluated samples from three APS patients and three control subjects for this proof-of-principle and already obtained good results for β2-glycoprotein I and cardiolipin.
authors.affiliation: Institute of Physical and Theoretical Chemistry, Eberhard Karls Universität, Auf der Morgenstelle 18, 72076, Tübingen, Germany, oliver.bleher@uni-tuebingen.de.
authors.forename: Oliver
authors.initials: O
authors.lastname: Bleher
authors.forename: Aline
authors.initials: A
authors.lastname: Schindler
authors.forename: Meng-Xin
authors.initials: MX
authors.lastname: Yin
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Holmes
authors.forename: Peter B
authors.initials: PB
authors.lastname: Luppa
authors.forename: Günter
authors.initials: G
authors.lastname: Gauglitz
authors.forename: Günther
authors.initials: G
authors.lastname: Proll
mesh_terms: D017153:Antibodies, Anticardiolipin / Q000276:immunology; D000937:Antigen-Antibody Reactions; D016736:Antiphospholipid Syndrome / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology*; D015551:Autoimmunity / Q000276:immunology*; D015374:Biosensing Techniques; D002308:Cardiolipins / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D004867:Equipment Design; D005898:Glass; D006801:Humans; D008857:Microscopy, Interference; D011516:Prothrombin / Q000737:chemistry; D012680:Sensitivity and Specificity; D012821:Silanes / Q000737:chemistry*; D013053:Spectrophotometry; D053482:beta 2-Glycoprotein I / Q000737:chemistry*
chemical_list: C000597752:11-aminoundecyltrimethoxysilane; D017153:Antibodies, Anticardiolipin; D001323:Autoantibodies; D002308:Cardiolipins; D012821:Silanes; D053482:beta 2-Glycoprotein I; D011516:Prothrombin