title: Bone Formation in a Local Defect around Dental Implants Coated with Extracellular Matrix Components.
abstract: OBJECTIVE
  The coating of implant surfaces with components of the extracellular matrix offers an approach to influence peri-implant bone healing. In this study, bone healing around coated implants is analyzed in a peri-implant defect model.
  
  
  METHODS
  Eight months after extraction of the premolar teeth, six dogs received 48 implants (eight per animal) in the mandible. Implant surfaces were sandblasted and acid-etched, and some were additionally coated with collagen type II and chondroitin sulfate (collagen/CS). On each side of the mandible, implants either had no peri-implant defect (control side) or a vertical defect of 5 mm in depth and 0.5, 1.0, or 2.0 mm in width. Implants healed submerged for 8 weeks. Fluorochrome staining, histology, and histomorphometry were used to analyze implant osseointegration.
  
  
  RESULTS
  Fluorochrome labels showed an increased mineralization around collagen/CS-coated surfaces at 4 weeks (p = .031). Histomorphometry generally showed lower vertical and horizontal bone apposition with increasing gap size for both surface types. In gapless sites and 0.5-mm gaps, collagen/CS coated implants showed increased bone volume in areas directly adjacent to the implant, in comparison with uncoated implants (p < .05).
  
  
  CONCLUSIONS
  The width of the peri-implant gap influences peri-implant bone formation. Complete filling of all gaps by newly formed bone could not be observed around either surface. In proximity to the surface, implant surface coating by collagen/CS positively influenced bone formation.
authors.affiliation: University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Raquel R M
authors.initials: RR
authors.lastname: de Barros
authors.affiliation: University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Arthur B
authors.initials: AB
authors.lastname: Novaes
authors.affiliation: Clinic of Oral & Maxillofacial Surgery, Technische Universität Dresden, Dresden, Germany.
authors.forename: Paula
authors.initials: P
authors.lastname: Korn
authors.affiliation: University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Adriana
authors.initials: A
authors.lastname: Queiroz
authors.affiliation: University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Adriana L G
authors.initials: AL
authors.lastname: de Almeida
authors.affiliation: Max Bergmann Center of Biomaterials, Technische Universität Dresden, Dresden, Germany.
authors.forename: Vera
authors.initials: V
authors.lastname: Hintze
authors.affiliation: Max Bergmann Center of Biomaterials, Technische Universität Dresden, Dresden, Germany.
authors.forename: Dieter
authors.initials: D
authors.lastname: Scharnweber
authors.affiliation: Max Bergmann Center of Biomaterials, Technische Universität Dresden, Dresden, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Bierbaum
authors.affiliation: Clinic of Cranio-Maxillofacial and Oral Surgery, University of Zurich, Zurich, Switzerland.
authors.forename: Bernd
authors.initials: B
authors.lastname: Stadlinger
mesh_terms: D000818:Animals; D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D017267:Dental Prosthesis Design / Q000379:methods*; D004285:Dogs; D005109:Extracellular Matrix / Q000737:chemistry*; D006801:Humans; D008856:Microscopy, Fluorescence; D016348:Osseointegration
chemical_list: D020099:Coated Materials, Biocompatible; D015921:Dental Implants
keywords: animal model; biocompatible; coated materials; dental implants; extracellular matrix; histology; osseointegration