title: Identification of differentially expressed genes in human mesenchymal stem cell-derived neurons.
abstract: Mesenchymal stem cells (MSCs) have greater potential for immediate clinical and toxicological applications, due to their ability to self-renew, proliferate, and differentiate into a variety of cell types. To identify novel candidate genes that were specifically expressed during transdifferentiation of human MSCs to neuronal cells, we performed a differential expression analysis with random priming approach using annealing control primer-based differential display reverse transcription-polymerase chain reaction approach. We identified genes for acyl-CoA thioesterase, tissue inhibitor of metalloproteinases-1, brain glycogen phosphorylase, ubiquitin C-terminal hydrolase and aldehyde reductase were up-regualted, whereas genes for transgelin and heparan sulfate proteoglycan were down-regulated in MSC-derived neurons. These differentially expressed genes may have potential role in regulation of neurogenesis. This study could be applied to environmental toxicology in the field of testing the toxicity of a chemical or a physical agent.
authors.affiliation: Department of Biomedical Science, College of Health Sciences, Korea University, Seoul 136-703, Korea.
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Heo
authors.forename: Kyung Jin
authors.initials: KJ
authors.lastname: Cho
authors.forename: Dal Woong
authors.initials: DW
authors.lastname: Choi
authors.forename: Suhng Wook
authors.initials: SW
authors.lastname: Kim
keywords: Differentially expressed genes; Mesenchymal stem cells; Neuron