title: Detection of protein S-sulfhydration by a tag-switch technique.
abstract: Protein S-sulfhydration (forming -S-SH adducts from cysteine residues) is a newly defined oxidative posttranslational modification and plays an important role in H2 S-mediated signaling pathways. In this study we report the first selective, "tag-switch" method which can directly label protein S-sulfhydrated residues by forming stable thioether conjugates. Furthermore we demonstrate that H2 S alone cannot lead to S-sulfhydration and that the two possible physiological mechanisms include reaction with protein sulfenic acids (P-SOH) or the involvement of metal centers which would facilitate the oxidation of H2 S to HS(.) .
authors.affiliation: Department of Chemistry, Washington State University, Pullman, WA 99164 (USA).
authors.forename: Dehui
authors.initials: D
authors.lastname: Zhang
authors.forename: Igor
authors.initials: I
authors.lastname: Macinkovic
authors.forename: Nelmi O
authors.initials: NO
authors.lastname: Devarie-Baez
authors.forename: Jia
authors.initials: J
authors.lastname: Pan
authors.forename: Chung-Min
authors.initials: CM
authors.lastname: Park
authors.forename: Kate S
authors.initials: KS
authors.lastname: Carroll
authors.forename: Milos R
authors.initials: MR
authors.lastname: Filipovic
authors.forename: Ming
authors.initials: M
authors.lastname: Xian
mesh_terms: D001710:Biotin / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry*; D005979:Glutathione Peroxidase / Q000737:chemistry; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000737:chemistry*; D019169:Jurkat Cells; D008856:Microscopy, Fluorescence; D011499:Protein Processing, Post-Translational; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry*
chemical_list: D013438:Sulfhydryl Compounds; D013440:Sulfides; C051552:persulfides; D012710:Serum Albumin, Bovine; D001710:Biotin; C486484:GPX3 protein, human; D005979:Glutathione Peroxidase; D003545:Cysteine; D006862:Hydrogen Sulfide
keywords: hydrogen sulfide; protein S-sulfhydration; signal transduction; tag-switch; thiols