title: Intestinal permeability of oxytetracycline from chitosan-montmorillonite nanocomposites.
abstract: A nanocomposite based on chitosan and montmorillonite was developed as carrier to improve oral bioavailability of oxytetracycline. The nanocomposite was prepared by simple solid-liquid interaction and loaded with the drug. The loaded nanocomposite was characterized by X-ray powder diffraction, thermal analysis, FTIR spectroscopy and zeta potential. Caco-2 cell cultures were used to evaluate in vitro cytotoxicity and drug permeation. Confocal laser scanning microscopy was also performed to evaluate the eventual entrapment of drug into the Caco-2 cells. Results showed that the nanocomposite was internalized into the cells and effectively enhanced drug permeation, being also biocompatible towards Caco-2 cells.
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Granada, Campus of Cartuja, 18071 s/n, Granada, Spain. Electronic address: isalcedo@ugr.es.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Salcedo
authors.affiliation: Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Sandri
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Granada, Campus of Cartuja, 18071 s/n, Granada, Spain.
authors.forename: Carola
authors.initials: C
authors.lastname: Aguzzi
authors.affiliation: Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Bonferoni
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Granada, Campus of Cartuja, 18071 s/n, Granada, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Cerezo
authors.affiliation: Andalusian Institute of Earth Sciences, CSIC-University of Granada, Avda. de Las Palmeras 4, 18100 Armilla, Granada, Spain.
authors.forename: Rita
authors.initials: R
authors.lastname: Sánchez-Espejo
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Granada, Campus of Cartuja, 18071 s/n, Granada, Spain; Andalusian Institute of Earth Sciences, CSIC-University of Granada, Avda. de Las Palmeras 4, 18100 Armilla, Granada, Spain.
authors.forename: César
authors.initials: C
authors.lastname: Viseras
mesh_terms: D001546:Bentonite / Q000737:chemistry*; D018938:Caco-2 Cells; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D017097:Electric Impedance; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects; D018613:Microscopy, Confocal; D053761:Nanocomposites / Q000737:chemistry*; D010118:Oxytetracycline / Q000494:pharmacology*; D010539:Permeability / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D001546:Bentonite; D048271:Chitosan; D010118:Oxytetracycline
keywords: Caco-2; Chitosan; Montmorillonite; Nanocomposites; Oxytetracycline