title: Over, and underexpression of endothelin 1 and TGF-beta family ligands and receptors in lung tissue of broilers with pulmonary hypertension.
abstract: Transforming growth factor beta (TGF β ) is a family of genes that play a key role in mediating tissue remodeling in various forms of acute and chronic lung disease. In order to assess their role on pulmonary hypertension in broilers, we determined mRNA expression of genes of the TGF β family and endothelin 1 in lung samples from 4-week-old chickens raised either under normal or cold temperature conditions. Both in control and cold-treated groups of broilers, endothelin 1 mRNA expression levels in lungs from ascitic chickens were higher than levels from healthy birds (P < 0.05), whereas levels in animals with cardiac failure were intermediate. Conversely, TGF β 2 and TGF β 3 gene expression in lungs were higher in healthy animals than in ascitic animals in both groups (P < 0.05). TGF β 1, T β RI, and T β RII mRNA gene expression among healthy, ascitic, and chickens with cardiac failure showed no differences (P > 0.05). BAMBI mRNA gene expression was lowest in birds with ascites only in the control group as compared with the values from healthy birds (P < 0.05).
authors.affiliation: Unidad Laguna, Universidad Autónoma Agraria Antonio Narro, 27010 Torreón, COAH, Mexico ; Posgrado del Centro de Estudios Justo Sierra, Surutato, 80600 Badiraguato, SIN, Mexico.
authors.forename: Norma
authors.initials: N
authors.lastname: Dominguez-Avila
authors.forename: Gabriel
authors.initials: G
authors.lastname: Ruiz-Castañeda
authors.forename: Javier
authors.initials: J
authors.lastname: González-Ramírez
authors.forename: Nora
authors.initials: N
authors.lastname: Fernandez-Jaramillo
authors.forename: Jorge
authors.initials: J
authors.lastname: Escoto
authors.forename: Fausto
authors.initials: F
authors.lastname: Sánchez-Muñoz
authors.forename: Ricardo
authors.initials: R
authors.lastname: Marquez-Velasco
authors.forename: Rafael
authors.initials: R
authors.lastname: Bojalil
authors.forename: Román
authors.initials: R
authors.lastname: Espinosa-Cervantes
authors.forename: Fausto
authors.initials: F
authors.lastname: Sánchez
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics*; D019332:Endothelin-1 / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D006976:Hypertension, Pulmonary / Q000235:genetics / Q000662:veterinary*; D008024:Ligands; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008297:Male; D011201:Poultry Diseases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000378:metabolism
chemical_list: D019332:Endothelin-1; D008024:Ligands; D012333:RNA, Messenger; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta