title: Intravital microscopy of the microcirculation in the mouse cremaster muscle for the analysis of peripheral stem cell migration.
abstract: In the era of intravascular cell application protocols in the context of regenerative cell therapy, the underlying mechanisms of stem cell migration to nonmarrow tissue have not been completely clarified. We describe here the technique of intravital microscopy applied to the mouse cremaster microcirculation for analysis of peripheral bone marrow stem cell migration in vivo. Intravital microscopy of the M. cremaster has been previously introduced in the field of inflammatory research for direct observation of leucocyte interaction with the vascular endothelium. Since sufficient peripheral stem and progenitor cell migration includes similar initial steps of rolling along and firm adhesion at the endothelial lining it is conceivable to apply the M. cremaster model for the observation and quantification of the interaction of intravasculary administered stem cells with the endothelium. As various chemical components can be selectively applied to the target tissue by simple superfusion techniques, it is possible to establish essential microenvironmental preconditions, for initial stem cell recruitment to take place in a living organism outside the bone marrow.
authors.affiliation: Reference and Translation Centre for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University Rostock.
authors.forename: Peter
authors.initials: P
authors.lastname: Donndorf
authors.forename: Marion
authors.initials: M
authors.lastname: Ludwig
authors.forename: Fabian
authors.initials: F
authors.lastname: Wildschütz
authors.forename: Dritan
authors.initials: D
authors.lastname: Useini
authors.forename: Alexander
authors.initials: A
authors.lastname: Kaminski
authors.forename: Brigitte
authors.initials: B
authors.lastname: Vollmar
authors.forename: Gustav
authors.initials: G
authors.lastname: Steinhoff
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002465:Cell Movement / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology*; D008297:Male; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D018715:Microscopy, Video / Q000379:methods*; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000166:cytology; D013234:Stem Cells / Q000166:cytology*