title: Synergistic and sequential effects of BMP-2, bFGF and VEGF on osteogenic differentiation of rat osteoblasts.
abstract: In the present study, the effects of bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) on regulation of rat osteoblast (ROB) maturation in vitro were investigated. It was found that the proliferation, differentiation and mineralization of ROBs were all dose-dependently increased at particular times in the case of treatment with only one growth factor. To investigate the effects of combined treatment, ROBs were treated with either a single application of a relatively high dose of each growth factor, or binary/triple combined applications of relatively low doses of the growth factors. Osteogenic differentiation was significantly promoted in the triple combination treatment of BMP-2, VEGF and bFGF compared with the single or binary combination treatments. The optimal timing of the triple combination to enhance osteogenesis was also tested. When bFGF and VEGF were added in the early stage, and BMP-2 and VEGF were added in the late stage, osteogenic differentiation of ROBs could be enhanced more effectively. These results could be used to construct bone tissue engineering scaffolds that release growth factors sequentially.
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu, 610064, People's Republic of China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Bai
authors.forename: Guangfu
authors.initials: G
authors.lastname: Yin
authors.forename: Ximing
authors.initials: X
authors.lastname: Pu
authors.forename: Zhongbing
authors.initials: Z
authors.lastname: Huang
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Liao
authors.forename: Xianchun
authors.initials: X
authors.lastname: Chen
authors.forename: Yadong
authors.initials: Y
authors.lastname: Yao
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000819:agonists / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D004357:Drug Synergism; D016222:Fibroblast Growth Factor 2 / Q000819:agonists / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D042461:Vascular Endothelial Growth Factor A / Q000819:agonists / Q000494:pharmacology*
chemical_list: C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D016222:Fibroblast Growth Factor 2