title: HPV genotyping 9G membrane test.
abstract: The results of the genital human papillomavirus (HPV) detection in 439 cervical samples by cervical cytology were compared with sequencing analysis and a newly developed HPV genotyping 9G membrane test. The excellent sensitivity and specificity of the HPV genotyping 9G membrane test was assured by a signal to noise ratio of more than 300 and a target hybridization to non-target hybridization ratio of 300 ~ 400 at 25 °C. The final results can be obtained in 29 min by simple loading of the hybridization and washing solutions and scanning the membranes without any drying steps or special handling. The 100% identical results of the HPV genotyping 9G membrane test with sequencing results in 439 clinical samples demonstrate significant clinical application for this test. HPV genotyping 9G membrane tests can identify and discriminate five HR-HPV genotypes which are prevalent in almost 87% of cervical cancer cases. Its simple handling makes the HPV genotyping 9G membrane test a very convenient platform for accurate HPV genotyping.
authors.affiliation: Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon, 200-702, Korea. tskim@hallym.ac.kr.
authors.forename: Danishmalik Rafiq
authors.initials: DR
authors.lastname: Sayyed
authors.forename: Keum-Soo
authors.initials: KS
authors.lastname: Song
authors.forename: Satish Balasaheb
authors.initials: SB
authors.lastname: Nimse
authors.forename: Heejung
authors.initials: H
authors.lastname: An
authors.forename: Junghoon
authors.initials: J
authors.lastname: Kim
authors.forename: Taisun
authors.initials: T
authors.lastname: Kim
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D008567:Membranes, Artificial; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology*; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral; D008567:Membranes, Artificial