title: Inter-α-inhibitor blocks epithelial sodium channel activation and decreases nasal potential differences in ΔF508 mice.
abstract: Increased activity of lung epithelial sodium channels (ENaCs) contributes to the pathophysiology of cystic fibrosis (CF) by increasing the rate of epithelial lining fluid reabsorption. Inter-α-inhibitor (IαI), a serum protease inhibitor, may decrease ENaC activity by preventing its cleavage by serine proteases. High concentrations of IαI were detected in the bronchoalveolar lavage fluid (BALF) of children with CF and lower airway diseases. IαI decreased amiloride-sensitive (IENaC) but not cAMP-activated Cl(-) currents across confluent monolayers of rat ATII, and mouse nasal epithelial cells grew in primary culture by 45 and 25%, respectively. Changes in IENaC by IαI in ATII cells were accompanied by increased levels of uncleaved (immature) surface α-ENaC. IαI increased airway surface liquid depth overlying murine nasal epithelial cells to the same extent as amiloride, consistent with ENaC inhibition. Incubation of lung slices from C57BL/6, those lacking phenylalanine at position 508 (∆F508), or CF transmembrane conductance regulator knockout mice with IαI for 3 hours decreased the open probability of their ENaC channels by 50%. ∆F508 mice had considerably higher levels the amiloride-sensitive fractions of ENaC nasal potential difference (ENaC-NPD) than wild-type littermates and only background levels of IαI in their BALF. A single intranasal instillation of IαI decreased their ENaC-NPD 24 hours later by 25%. In conclusion, we show that IαI is present in the BALF of children with CF, is an effective inhibitor of ENaC proteolysis, and decreases ENaC activity in lung epithelial cells of ∆F508 mice.
authors.affiliation: Departments of 1 Anesthesiology.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Lazrak
authors.forename: Asta
authors.initials: A
authors.lastname: Jurkuvenaite
authors.forename: Emily C
authors.initials: EC
authors.lastname: Ness
authors.forename: Shaoyan
authors.initials: S
authors.lastname: Zhang
authors.forename: Bradford A
authors.initials: BA
authors.lastname: Woodworth
authors.forename: Marianne S
authors.initials: MS
authors.lastname: Muhlebach
authors.forename: Vandy P
authors.initials: VP
authors.lastname: Stober
authors.forename: Yow-Pin
authors.initials: YP
authors.lastname: Lim
authors.forename: Stavros
authors.initials: S
authors.lastname: Garantziotis
authors.forename: Sadis
authors.initials: S
authors.lastname: Matalon
mesh_terms: D000510:Alpha-Globulins / Q000378:metabolism*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D002478:Cells, Cultured; D003550:Cystic Fibrosis / Q000378:metabolism; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism*; D062708:Epithelial Sodium Channel Agonists / Q000378:metabolism*; D053503:Epithelial Sodium Channels / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009865:Oocytes / Q000378:metabolism; D051381:Rats; D014982:Xenopus laevis / Q000378:metabolism
chemical_list: D000510:Alpha-Globulins; D062708:Epithelial Sodium Channel Agonists; D053503:Epithelial Sodium Channels; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; C011877:inter-alpha-inhibitor