title: Contribution of molecular diagnosis to the management of cutaneous leishmaniasis in travellers.
abstract: Cutaneous leishmaniasis is one of the most frequent skin diseases occurring after travelling in endemic areas. Optimal management requires identification of the species of Leishmania involved. In this study we aimed to evaluate the use of molecular diagnosis as routine, in comparison with direct examination and culture. Thirty positive diagnoses were carried out between 2007 and 2013. Classical PCR enabled 11 positive cases to be identified that were found to be negative by conventional methods. Sequencing led to the identification of eight different species. Routine use of PCR and sequencing appears very efficient in the management of cutaneous leishmaniasis.
authors.affiliation: Service de Parasitologie-Mycologie, Centre Hospitalier Universitaire de Toulouse, Toulouse, France; INSERM, U1043, Toulouse, France; CNRS, U5282, Toulouse, France; Université de Toulouse, UPS, Centre de Physiopathologie de Toulouse Purpan (CPTP), Toulouse, France.
authors.forename: R A
authors.initials: RA
authors.lastname: Lavergne
authors.forename: X
authors.initials: X
authors.lastname: Iriart
authors.forename: G
authors.initials: G
authors.lastname: Martin-Blondel
authors.forename: P
authors.initials: P
authors.lastname: Chauvin
authors.forename: S
authors.initials: S
authors.lastname: Menard
authors.forename: J
authors.initials: J
authors.lastname: Fillaux
authors.forename: S
authors.initials: S
authors.lastname: Cassaing
authors.forename: C
authors.initials: C
authors.lastname: Roques-Malecaze
authors.forename: S
authors.initials: S
authors.lastname: Arnaud
authors.forename: A
authors.initials: A
authors.lastname: Valentin
authors.forename: J F
authors.initials: JF
authors.lastname: Magnaval
authors.forename: B
authors.initials: B
authors.lastname: Marchou
authors.forename: A
authors.initials: A
authors.lastname: Berry
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007891:Leishmania / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods; D014195:Travel; D055815:Young Adult
keywords: Cutaneous; PCR; diagnosis; leishmaniasis; sequencing