title: Antisense effects of PNAs in bacteria.
abstract: Peptide nucleic acids (PNAs) are a class of artificial DNA/RNA analogues that have unique physicochemical properties, which include a high chemical stability, resistance to nucleases and proteases and higher mismatch sensitivity than DNA. PNAs were initially anticipated to be useful for application in antisense and antigene therapies; however, their poor cellular uptake has limited their use for such purposes in the "real world". Recently, it has been shown that the addition of metal complexes to these oligonucleotide analogues could open up new avenues for their utilization in various research fields. Such metallo-constructs have shown great promise, for a diverse range of applications, most notably in the biosensing area. In this chapter, we report on the recent synthetic advances towards the preparation of these "(multi)-metallic PNAs" on the solid phase.
authors.affiliation: Department of Pathology and Infectious Diseases, Royal Veterinary College, University of London, London, UK.
authors.forename: Shan
authors.initials: S
authors.lastname: Goh
authors.forename: Jem
authors.initials: J
authors.lastname: Stach
authors.forename: Liam
authors.initials: L
authors.lastname: Good
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D057846:Cell-Penetrating Peptides / Q000737:chemistry / Q000378:metabolism; D048168:Escherichia coli K12 / Q000235:genetics*; D020868:Gene Silencing; D017930:Genes, Reporter / Q000235:genetics; D016376:Oligonucleotides, Antisense / Q000235:genetics* / Q000378:metabolism; D020135:Peptide Nucleic Acids / Q000235:genetics* / Q000378:metabolism; D015203:Reproducibility of Results
chemical_list: D057846:Cell-Penetrating Peptides; D016376:Oligonucleotides, Antisense; D020135:Peptide Nucleic Acids