title: [Modified TRIzol method for RNA and DNA co-extraction from blood].
abstract: OBJECTIVE
  To establish a new method for RNA and DNA co-extraction from the same sample by TRIzol reagent.
  
  
  METHODS
  After the aqueous phase which contained total RNA was removed by traditional TRIzol method, the values of pH of the interphase phase and organic phase were adjusted. The DNA was precipitated with ethanol and purified with DNA IQ system. The purified DNA was measured in quality and quantity. As the template, it was amplified and typed by PCR-STR. The data was compared with that extracted by traditional TRIzol method.
  
  
  RESULTS
  The DNA extracted by this modified method showed a better result of quality and quantity than that by traditional TRIzol method and a good STR typing.
  
  
  CONCLUSIONS
  The modified TRIzol method is advisable and reliable to simultaneously extract both DNA and RNA from the same sample. It could be used for individual identification and paternity testing to satisfy the need of forensic science.
authors.affiliation: Department of Forensic Medicine, School of Basic Medicine, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Juan-Juan
authors.initials: JJ
authors.lastname: Qin
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Lu
authors.forename: Zhang-Ping
authors.initials: ZP
authors.lastname: Jiao
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Zhu
authors.forename: Ying-Xi
authors.initials: YX
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Tang
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D004247:DNA / Q000097:blood / Q000302:isolation & purification*; D016172:DNA Fingerprinting; D005554:Forensic Medicine; D006146:Guanidines / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010636:Phenols / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000097:blood / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic
chemical_list: D006146:Guanidines; D010636:Phenols; D011933:Reagent Kits, Diagnostic; C411644:trizol; D012313:RNA; D004247:DNA