title: Determination of YZG-331 in mouse plasma using liquid chromatography-tandem mass spectrometry.
abstract: A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of YZG-331 in mouse plasma was developed. Plasma samples containing YZG-331 and YZG-441 (internal standard, IS) were prepared using a simple protein precipitation by the addition of acetonitrile. Thermo Scientific TSQ Quantum triple quadrupole system with multiple reaction monitoring (MRM) positive scanning mode was applied. The separation was performed on a ZorbaxSB-C18 column (3.5μm, 2.1mm×100mm) at a flow rate of 0.2mL/min, using acetonitrile/water containing 0.1% formic acid (v/v) as mobile phase. The MS/MS ion transit ions monitored were 386→254 for YZG-331 and 400→268 for IS. Linear detection responses were obtained for YZG-331 ranging from 25 to 5000ng/mL and the lower limits of quantitation (LLOQ) for the compound was 25ng/mL. The intra- and inter-day precisions (R.S.D.%) were within 12.6% for all analytes, while the deviation of assay accuracies was within ±6.9%. The average recoveries of analytes were greater than 94.3%. The analytes were proved to be stable during all sample storage, preparation and analytic procedures. The method was successfully applied to the pharmacokinetic studies of YZG-331 in mice.
authors.affiliation: Department of Drug Metabolism, Key Laboratory of Active Substances Discovery and Drug Ability Evaluation, State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Drug Metabolism, Key Laboratory of Active Substances Discovery and Drug Ability Evaluation, State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
authors.forename: Li
authors.initials: L
authors.lastname: Sheng
authors.affiliation: Department of Drug Metabolism, Key Laboratory of Active Substances Discovery and Drug Ability Evaluation, State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China. Electronic address: yanli@imm.ac.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
mesh_terms: D000097:Acetonitriles; D000241:Adenosine / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000097:Acetonitriles; C000589454:YZG-331; D000241:Adenosine; C032159:acetonitrile
keywords: LC–MS/MS; Mouse; Pharmacokinetic; YZG-331