title: Encapsulation of an enzyme cascade within the bacteriophage P22 virus-like particle.
abstract: Developing methods for investigating coupled enzyme systems under conditions that mimic the cellular environment remains a significant challenge. Here we describe a biomimetic approach for constructing densely packed and confined multienzyme systems through the co-encapsulation of 2 and 3 enzymes within a virus-like particle (VLP) that perform a coupled cascade of reactions, creating a synthetic metabolon. Enzymes are efficiently encapsulated in vivo with known stoichiometries, and the kinetic parameters of the individual and coupled activities are characterized. From the results we develop and validate a mathematical model for predicting the expected kinetics for coupled reactions under co-localized conditions.
authors.affiliation: Department of Chemistry and Biochemistry, ‡Center for Bio-Inspired Nanomaterials, and §Deparment of Mathematical Sciences, Montana State University , Bozeman, Montana 59717, United States.
authors.forename: Dustin P
authors.initials: DP
authors.lastname: Patterson
authors.forename: Benjamin
authors.initials: B
authors.lastname: Schwarz
authors.forename: Ryan S
authors.initials: RS
authors.lastname: Waters
authors.forename: Tomas
authors.initials: T
authors.lastname: Gedeon
authors.forename: Trevor
authors.initials: T
authors.lastname: Douglas
mesh_terms: D017100:Bacteriophage P22 / Q000737:chemistry / Q000201:enzymology*; D032701:Biomimetics / Q000379:methods*; D002213:Capsid / Q000737:chemistry / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008954:Models, Biological; D008958:Models, Molecular; D009097:Multienzyme Complexes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004800:Enzymes, Immobilized; D009097:Multienzyme Complexes