title: Comparison of Bovine coronavirus-specific and Bovine respiratory syncytial virus-specific antibodies in serum versus milk samples detected by enzyme-linked immunosorbent assay.
abstract: Bovine coronavirus (BCV; Betacoronavirus 1) and Bovine respiratory syncytial virus (BRSV) are significant causes of enteric and respiratory disease in beef and dairy cattle throughout the world. Indirect enzyme-linked immunosorbent assays are widely used to detect serum antibodies for herd monitoring and prevalence studies. In dairy herds, milk is more readily collected than serum. Hence, in order to investigate the test agreement between serum and milk, both serum and milk samples from 105 cows in 27 dairy herds were analyzed in parallel for presence of immunoglobulin G antibodies to BCV and BRSV. The Bland-Altman analyses of data demonstrated good agreement between serum and milk antibody titers for both viruses. The results indicate milk samples are sufficient for surveillance of antibodies to BCV and BRSV.
authors.affiliation: 1Anna Ohlson, Växa Sverige, Box 210, 101 24 Stockholm, Sweden. anna.ohlson@vxa.se.
authors.forename: Anna
authors.initials: A
authors.lastname: Ohlson
authors.forename: Isabel
authors.initials: I
authors.lastname: Blanco-Penedo
authors.forename: Nils
authors.initials: N
authors.lastname: Fall
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000821:virology*; D018352:Coronavirus Infections / Q000097:blood / Q000662:veterinary* / Q000821:virology; D017938:Coronavirus, Bovine / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007074:Immunoglobulin G / Q000032:analysis; D008892:Milk / Q000821:virology*; D015203:Reproducibility of Results; D018357:Respiratory Syncytial Virus Infections / Q000097:blood / Q000662:veterinary* / Q000821:virology; D018112:Respiratory Syncytial Virus, Bovine / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D007074:Immunoglobulin G
keywords: Antibody correlation; Bovine coronavirus; Bovine respiratory syncytial virus; antibody evaluation; enzyme-linked immunosorbent assay; milk; serum