title: Kaempferol promotes apoptosis in human bladder cancer cells by inducing the tumor suppressor, PTEN.
abstract: Kaempferol (Kae), a natural flavonoid, is widely distributed in fruits and vegetables. Previous studies have identified Kae as a possible cancer preventive and therapeutic agent. We found Kae to exhibit potent antiproliferation and anti-migration effects in human bladder cancer EJ cells. Kaempferol robustly induced apoptosis in EJ cells in a dose-dependent manner, as evidenced by increased cleavage of caspase-3. Furthermore, we found Kae-induced apoptosis in EJ cells to be associated with phosphatase and the tensin homolog deleted on the chromosome 10 (PTEN)/PI3K/Akt pathway. Kae significantly increased PTEN and decreased Akt phosphorylation. Kae-induced apoptosis was partially attenuated in PTEN-knockdown cells. Our findings indicate that Kae could be an alternative medicine for bladder cancer, based on a PTEN activation mechanism.
authors.affiliation: Department of Urology, Peking University First Hospital & the Institute of Urology, Peking University, Beijing 100034, China. zhoulqmail@china.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Xie
authors.forename: Ming
authors.initials: M
authors.lastname: Su
authors.forename: Wei
authors.initials: W
authors.lastname: Qiu
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Zhongqiang
authors.initials: Z
authors.lastname: Guo
authors.forename: Boxing
authors.initials: B
authors.lastname: Su
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Xuesong
authors.initials: X
authors.lastname: Li
authors.forename: Liqun
authors.initials: L
authors.lastname: Zhou
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D044949:Kaempferols / Q000008:administration & dosage*; D051059:PTEN Phosphohydrolase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy* / Q000473:pathology
chemical_list: D044949:Kaempferols; C006552:kaempferol; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human; D053148:Caspase 3