title: Cytotoxicity Evaluation of Proroot MTA, Root MTA and Portland Cement on Human Gingival Fibroblasts.
abstract: BACKGROUND
  The aim of this study was to compare the cytotoxicity of ProRoot MTA (PMTA), Root MTA (RMTA) and Portland cement (PC) on human gingival fibroblasts (HGFs).
  
  
  METHODS
  The extracts of the test materials were placed close to human gingival fibroblasts (HGFs) within 96-well plates. Cellular viability was assessed using MTT assay in different intervals (freshly mixed, 4, 24, and 168 hours after mixing). The data were analyzed using the One-way ANOVA and Tukey's test at 95% significance level.
  
  
  RESULTS
  It was indicated that there was not a significant difference in cytotoxicity of test materials (p>0.05). In addition, there was not a statistically significant difference between different time intervals within each group (p>0.05).
  
  
  CONCLUSIONS
  PMTA, RMTA and PC showed comparative biocompatibility while evaluated in-vitro.
authors.affiliation: Department of Endodontics, School of Dentistry, Dental Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Sharifian
authors.forename: Mehrnoosh
authors.initials: M
authors.lastname: Ghobadi
authors.forename: Noushin
authors.initials: N
authors.lastname: Shokouhinejad
authors.forename: Hadi
authors.initials: H
authors.lastname: Assadian
keywords: Cytotoxicity; Mineral Trioxide Aggregate; Portland Cement