title: Phenotypic and functional heterogeneity of CD169⁺ and CD163⁺ macrophages from porcine lymph nodes and spleen.
abstract: Secondary lymphoid organ macrophages are involved in the establishment of innate and acquired immunity. Here, we have isolated and characterized porcine lymph node and spleen CD169(+) and spleen CD163(+) macrophages. Lymph node and spleen CD169(+) macrophages can be both identified as CD172a(+)SLA-DR(hi)CD80/86(hi)CD14(int)TLR2(+)TLR4(+). On the other side, spleen CD163(+) macrophages are CD172a(+)SLA-DR(int)CD80/86(int)CD14(-)/(lo)TLR2(int)TLR4(int). In addition, these macrophages can be subdivided based on the expression of CD11R1 or CD11R3. Lymph node CD169(+) macrophages phagocytozed polystyrene microspheres more efficiently than spleen CD163(+) and CD169(+) macrophages. All macrophages exhibited low capacity to take up and process the soluble antigen DQ-OVA. Finally, spleen CD163(+) macrophages displayed the highest ability to present lysozyme to CD4(+) T cells in a secondary in vitro response, followed by lymph node and spleen CD169(+) macrophages.
authors.affiliation: Dpto. Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, Spain.
authors.forename: Belén
authors.initials: B
authors.lastname: Alvarez
authors.affiliation: Dpto. Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, Spain.
authors.forename: Paloma
authors.initials: P
authors.lastname: Martínez
authors.affiliation: Dpto. Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Yuste
authors.affiliation: Dpto. Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Poderoso
authors.affiliation: Dpto. Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Alonso
authors.affiliation: Dpto. Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Domínguez
authors.affiliation: Dpto. Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, Spain.
authors.forename: Angel
authors.initials: A
authors.lastname: Ezquerra
authors.affiliation: Dpto. Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, Spain. Electronic address: crevilla@inia.es.
authors.forename: Concepción
authors.initials: C
authors.lastname: Revilla
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D008198:Lymph Nodes / Q000166:cytology*; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology*; D010587:Phagocytosis; D010641:Phenotype; D011956:Receptors, Cell Surface / Q000378:metabolism; D063267:Sialic Acid Binding Ig-like Lectin 1 / Q000378:metabolism; D013154:Spleen / Q000166:cytology*; D013552:Swine / Q000276:immunology*; D051195:Toll-Like Receptor 2 / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D000098902:CD163 Antigen
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; D011956:Receptors, Cell Surface; D063267:Sialic Acid Binding Ig-like Lectin 1; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4
keywords: Antigen presentation; Lymph nodes; Macrophages; Pig; Spleen