title: Screening chemical libraries for compounds that affect protein sorting to the yeast vacuole.
abstract: Protein trafficking to the yeast vacuole has been extensively studied using a series of deletion mutants. In these genetic screens, mis-targeted vacuolar cargo proteins were used as phenotype markers. Here we describe a similar approach employing pharmacological effects of diverse chemical compounds to mimic molecular phenotypes caused by conventional genetic mutations in protein trafficking genes.
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Pozuelo de Alarcón, España.
authors.forename: Jan
authors.initials: J
authors.lastname: Zouhar
mesh_terms: D004353:Drug Evaluation, Preclinical; D021381:Protein Transport / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D054852:Small Molecule Libraries; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D054852:Small Molecule Libraries