title: Upregulation of SYF2 is associated with neuronal apoptosis caused by reactive astrogliosis to neuroinflammation.
abstract: SYF2, known as CCNDBP1-interactor or p29, is likely involved in pre-mRNA splicing and cell cycle progression. The present study was designed to elucidate dynamic changes in SYF2 expression and distribution in the cerebral cortex in a lipopolysaccharide (LPS)-induced neuroinflammation rat model. It was found that SYF2 expression was induced strongly in active astrocytes after LPS injection. In vitro studies showed that the upregulation of SYF2 might be involved in the activation of C6 cells after LPS challenge and the neuronal apoptosis after conditioned media challenge. In addition, with silencing of SYF2 in C6 and PC12 cells by siRNA, the results indicated that SYF2 was required for astrocyte activation and neuronal apoptosis induced by LPS. Our findings on the cellular signaling pathway may provide a new therapeutic strategy against neuroinflammation in the CNS.
authors.affiliation: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Maohong
authors.initials: M
authors.lastname: Cao
authors.forename: Heyi
authors.initials: H
authors.lastname: Zheng
authors.forename: Xiang
authors.initials: X
authors.lastname: Tan
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Gang
authors.initials: G
authors.lastname: Cui
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Cao
authors.forename: Kaifu
authors.initials: K
authors.lastname: Ke
authors.forename: Qiyun
authors.initials: Q
authors.lastname: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D001921:Brain / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004195:Disease Models, Animal; D004660:Encephalitis / Q000139:chemically induced / Q000150:complications* / Q000473:pathology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005911:Gliosis / Q000209:etiology*; D008070:Lipopolysaccharides; D008297:Male; D009457:Neuroglia / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000473:pathology*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley; D015533:Transcriptional Activation / Q000187:drug effects; D014162:Transfection; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology*
chemical_list: D017077:Culture Media, Conditioned; C418608:GCIP-interacting protein p29, human; D005904:Glial Fibrillary Acidic Protein; D008070:Lipopolysaccharides; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins
keywords: SYF2; astrocyte proliferation; cyclin D1; neuroinflammation; neuronal apoptosis; rat