title: MicroRNA-590 promotes cervical cancer cell growth and invasion by targeting CHL1.
abstract: MicroRNAs (miRNAs) may function as oncogenes or tumor suppressors. Here, we identified that miR-590-5p was up-regulated in human cervical cancer. Over-expression of miR-590-5p promoted cervical cancer cell growth, cell cycle and invasion via Growth curve, Colony formation, FACS and Transwell assays in HeLa and C33A cell lines. Subsequently, CHL1 was identified as a potential miR-590-5p target by bioinformatics analysis. Moreover, we showed that CHL1 was negatively regulated by miR-590-5p at the posttranscriptional level, via a specific target site within the 3'UTR by luciferase reporter assay. Furthermore, the mRNA and protein levels of CHL1 in cervical cancer cells were downregulated by miR-590-5p. And we identified the cell phenotype altered by miR-590-5p can be rescued by over-expression of CHL1. Therefore, our findings suggest that miR-590-5p acts as an oncogene by targeting the CHL1 gene and promotes cervical cancer proliferation. The findings of this study contribute to current understanding of the functions of miR-590-5p in cervical cancer.
authors.affiliation: Department of Obstetric and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, 610041, China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Chu
authors.forename: Yunwei
authors.initials: Y
authors.lastname: Ouyang
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Ai
authors.initials: A
authors.lastname: Zheng
authors.forename: Liping
authors.initials: L
authors.lastname: Bai
authors.forename: Ling
authors.initials: L
authors.lastname: Han
authors.forename: Yali
authors.initials: Y
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
mesh_terms: D020413:3' Untranslated Regions; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; C490650:CHL1 protein, human; D015815:Cell Adhesion Molecules; C568221:MIRN590 microRNA, human; D035683:MicroRNAs
keywords: CELL CYCLE; CELL INVASION; CERVICAL CANCER; CHL1; miR-590-5p