title: The protective role of Bax inhibitor-1 against chronic mild stress through the inhibition of monoamine oxidase A.
abstract: The anti-apoptotic protein Bax inhibitor-1 (BI-1) is a regulator of apoptosis linked to endoplasmic reticulum (ER) stress. It has been hypothesized that BI-1 protects against neuron degenerative diseases. In this study, BI-1⁻/⁻ mice showed increased vulnerability to chronic mild stress accompanied by alterations in the size and morphology of the hippocampi, enhanced ROS accumulation and an ER stress response compared with BI-1⁺/⁺ mice. BI-1⁻/⁻ mice exposed to chronic mild stress showed significant activation of monoamine oxidase A (MAO-A), but not MAO-B, compared with BI-1⁺/⁺ mice. To examine the involvement of BI-1 in the Ca²⁺-sensitive MAO activity, thapsigargin-induced Ca²⁺ release and MAO activity were analyzed in neuronal cells overexpressing BI-1. The in vitro study showed that BI-1 regulates Ca²⁺ release and related MAO-A activity. This study indicates an endogenous protective role of BI-1 under conditions of chronic mild stress that is primarily mediated through Ca²⁺-associated MAO-A regulation.
authors.affiliation: 1] Department of Pharmacology, Chonbuk National University Medical School, Jeonju, Korea [2].
authors.forename: Hwa-Young
authors.initials: HY
authors.lastname: Lee
authors.forename: Geum-Hwa
authors.initials: GH
authors.lastname: Lee
authors.forename: Anu
authors.initials: A
authors.lastname: Marahatta
authors.forename: Shun-Mei
authors.initials: SM
authors.lastname: Lin
authors.forename: Mi-Rin
authors.initials: MR
authors.lastname: Lee
authors.forename: Kyu Yun
authors.initials: KY
authors.lastname: Jang
authors.forename: Kyung Min
authors.initials: KM
authors.lastname: Kim
authors.forename: Hee Jae
authors.initials: HJ
authors.lastname: Lee
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Tarique Rajasaheb
authors.initials: TR
authors.lastname: Bagalkot
authors.forename: Young-Chul
authors.initials: YC
authors.lastname: Chung
authors.forename: Yong-Chul
authors.initials: YC
authors.lastname: Lee
authors.forename: Hyung-Ryong
authors.initials: HR
authors.lastname: Kim
authors.forename: Han-Jung
authors.initials: HJ
authors.lastname: Chae
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D005260:Female; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008995:Monoamine Oxidase / Q000378:metabolism*; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D019284:Thapsigargin / Q000494:pharmacology
chemical_list: D008565:Membrane Proteins; D008996:Monoamine Oxidase Inhibitors; D017382:Reactive Oxygen Species; C490015:Tmbim6 protein, mouse; D019284:Thapsigargin; D008995:Monoamine Oxidase; D002118:Calcium