title: Replication and packaging of Turnip yellow mosaic virus RNA containing Flock house virus RNA1 sequence.
abstract: Turnip yellow mosaic virus (TYMV) is a spherical plant virus that has a single 6.3 kb positive strand RNA as a genome. In this study, RNA1 sequence of Flock house virus (FHV) was inserted into the TYMV genome to test whether TYMV can accommodate and express another viral entity. In the resulting construct, designated TY-FHV, the FHV RNA1 sequence was expressed as a TYMV subgenomic RNA. Northern analysis of the Nicotiana benthamiana leaves agroinfiltrated with the TY-FHV showed that both genomic and subgenomic FHV RNAs were abundantly produced. This indicates that the FHV RNA1 sequence was correctly expressed and translated to produce a functional FHV replicase. Although these FHV RNAs were not encapsidated, the FHV RNA having a TYMV CP sequence at the 3'-end was efficiently encapsidated. When an eGFP gene was inserted into the B2 ORF of the FHV sequence, a fusion protein of B2-eGFP was produced as expected.
authors.affiliation: Department of Biochemistry, Chungbuk National University, Cheongju 361-763, Korea.
authors.forename: Hui-Bae
authors.initials: HB
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, Chungbuk National University, Cheongju 361-763, Korea.
authors.forename: Do-Yeong
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, Chungbuk National University, Cheongju 361-763, Korea.
authors.forename: Tae-Ju
authors.initials: TJ
authors.lastname: Cho
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000821:virology; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D014026:Nicotiana / Q000821:virology; D017806:Tymovirus / Q000235:genetics / Q000502:physiology*; D019065:Virus Assembly; D014779:Virus Replication
chemical_list: D036022:Capsid Proteins; C042027:RNA I; D012329:RNA, Bacterial; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D012324:RNA-Dependent RNA Polymerase