title: Long noncoding RNAs: fresh perspectives into the RNA world.
abstract: Large-scale mapping of transcriptomes has revealed significant levels of transcriptional activity within both unannotated and annotated regions of the genome. Interestingly, many of the novel transcripts demonstrate tissue-specific expression and some level of sequence conservation across species, but most have low protein-coding potential. Here, we describe progress in identifying and characterizing long noncoding RNAs (lncRNAs) and review how these transcripts interact with other biological molecules to regulate diverse cellular processes. We also preview emerging techniques that will help advance the discovery and characterization of novel transcripts. Finally, we discuss the role of lncRNAs in disease and therapeutics.
authors.affiliation: Howard Hughes Medical Institute, Massachusetts General Hospital, Boston, MA 02114, USA; Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA; Department of Genetics, Harvard Medical School, Boston, MA 02114, USA.
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.affiliation: Howard Hughes Medical Institute, Massachusetts General Hospital, Boston, MA 02114, USA; Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA; Department of Genetics, Harvard Medical School, Boston, MA 02114, USA.
authors.forename: John E
authors.initials: JE
authors.lastname: Froberg
authors.affiliation: Howard Hughes Medical Institute, Massachusetts General Hospital, Boston, MA 02114, USA; Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA; Department of Genetics, Harvard Medical School, Boston, MA 02114, USA. Electronic address: lee@molbio.mgh.harvard.edu.
authors.forename: Jeannie T
authors.initials: JT
authors.lastname: Lee
mesh_terms: D000818:Animals; D042002:Chromatin Assembly and Disassembly; D018392:Genomic Imprinting; D006801:Humans; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012323:RNA Processing, Post-Transcriptional; D062085:RNA, Long Noncoding / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D062085:RNA, Long Noncoding; C077885:XIST non-coding RNA
keywords: Long noncoding RNA; epigenetics; next-generation sequencing; therapeutics