title: Cholecystokinin receptors in Atlantic salmon: molecular cloning, gene expression, and structural basis.
abstract: The peptide hormone cholecystokinin (CCK) exerts a wide range of digestive and CNS-related physiological signaling via CCK receptors in brain and gut. There is very limited information available on these receptors in Atlantic salmon. The aim of this study was to characterize CCK receptors in gut and brain of salmon. We have identified and cloned one CCK-1 receptor and duplicates of CCK-2 receptor in salmon. The phylogenetic analysis indicates the existence of one common ancestor gene for all CCK receptors. CCK-1R mRNA is highly expressed in pancreas followed by midgut, hindgut, gallbladder, and stomach indicating an involvement in pancreatic regulation and gallbladder contractions. CCK-2R1/gastrin mRNA is expressed at high levels in midgut and at relatively low levels in stomach, gallbladder, and pancreas. We postulate CCK-2R1/gastrin receptor to have gastrin-related functions because of its distribution and abundance in gastro-intestinal (GI) tissues. CCK-2R2 is relatively abundant in brain but has low expression levels in gut tissues supporting the hypothesis for involvement in the gut-brain signaling. Major functional motifs and ligand interaction sites in salmon are conserved with that of mammals. This information will be instrumental for comparative studies and further targeting receptor activation and selectivity of biological responses of CCK in salmon.
authors.affiliation: Department of Biology, University of Bergen N-5020, Bergen, Norway.
authors.forename: Raja M
authors.initials: RM
authors.lastname: Rathore
authors.forename: Anna R
authors.initials: AR
authors.lastname: Angotzi
authors.forename: Ann-Elise O
authors.initials: AE
authors.lastname: Jordal
authors.forename: Ivar
authors.initials: I
authors.lastname: Rønnestad
keywords: Appetite regulation; Atlantic salmon; cholecystokinin receptors; digestive physiology; gene expression; ligand binding; protein structure