title: β-Glucuronidase and β-glucosidase activity and human fecal water genotoxicity in the presence of probiotic lactobacilli and the heterocyclic aromatic amine IQ in vitro.
abstract: The aim of the study was to assess the genotoxicity of fecal water (FW) and the activity of fecal enzymes (β-glucuronidase and β-glucosidase) after incubation with 2-amino-3-methyl-3H-imidazo[4,5-f]quinoline (IQ) and probiotic lactobacilli: Lb. casei 0900, Lb. casei 0908, and Lb. paracasei 0919. Our results show that the carcinogen IQ greatly increased FW genotoxicity (up to 16.92 ± 3.03 U/mg) and the activity of fecal enzymes (up to even 1.4 ± 0.16 U/mg) in 15 individuals (children, adults and elderly). After incubation with IQ, the activity of β-glucuronidase was reduced by Lactobacillus bacteria by 76.0% (Lb. paracasei 0908) in the FW of children, and by 82.0% (Lb. paracasei 0919) in the elderly; while that of β-glucosidase was reduced by 55.0% in children (Lb. casei 0908) and 90.0% (Lb. paracasei 0919) in elderly subjects. Lactobacilli decreased the genotoxicity of FW after incubation with IQ to the greatest extent in adults (by 64.5%). Probiotic lactobacilli, in the presence of IQ, efficiently inhibits activity of fecal enzymes to the level of control. Genotoxicity inhibition depends on the person's age, its individual microbiota and diet.
authors.affiliation: Institute of Fermentation Technology and Microbiology, Department of Biotechnology and Food Sciences, Lodz University of Technology, Wolczanska 171/173, 90-924 Lodz, Poland. Electronic address: adriana.nowak@p.lodz.pl.
authors.forename: Adriana
authors.initials: A
authors.lastname: Nowak
authors.affiliation: Institute of Fermentation Technology and Microbiology, Department of Biotechnology and Food Sciences, Lodz University of Technology, Wolczanska 171/173, 90-924 Lodz, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Śliżewska
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018938:Caco-2 Cells; D002273:Carcinogens / Q000633:toxicity*; D004249:DNA Damage; D005243:Feces; D005260:Female; D005966:Glucuronidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006801:Humans; D007093:Imidazoles / Q000633:toxicity*; D007223:Infant; D007231:Infant, Newborn; D007778:Lactobacillus; D008297:Male; D019936:Probiotics / Q000494:pharmacology*; D011804:Quinolines / Q000633:toxicity*; D014873:Water Pollutants; D001617:beta-Glucosidase / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: C546460:2-amino-3-methyl-3H-imidazo(4,5-f)quinoline; D002273:Carcinogens; D007093:Imidazoles; D011804:Quinolines; D014873:Water Pollutants; D001617:beta-Glucosidase; D005966:Glucuronidase
keywords: Fecal water; Genotoxicity; IQ; Probiotics; β-Glucosidase; β-Glucuronidase