title: Persistent sodium current properties in hippocampal CA1 pyramidal neurons of young and adult rats.
abstract: Persistent tetrodotoxin-sensitive sodium current (INaP) plays an important role in cellular and neuronal network excitability in physiological conditions and under different pathological circumstances. However, developmental changes in INaP properties remain largely unclear. In the present study using whole cell patch clamp technique we evaluated INaP properties in CA1 hippocampal pyramidal neurons isolated from young (postnatal day (P) 12-16) and adult (P60-75) rats. We show that the INaP density is substantially larger in the adult group. Although INaP inactivation characteristics were found to be similar in both groups, voltage dependence of INaP activation is shifted to more negative membrane potentials (young: -48.6±0.5mV vs. adult: -52.4±0.2mV, p<0.01). Our data indicates the increase of INaP contribution in the basal membrane sodium conductivity in the mature hippocampus.
authors.affiliation: Department of Cellular Membranology, Bogomoletz Institute of Physiology, Kiev 01024, Ukraine.
authors.forename: Oleksii
authors.initials: O
authors.lastname: Lunko
authors.affiliation: Department of Cellular Membranology, Bogomoletz Institute of Physiology, Kiev 01024, Ukraine; State Key Laboratory for Molecular and Cellular Biology, Kiev 01024, Ukraine.
authors.forename: Dmytro
authors.initials: D
authors.lastname: Isaev
authors.affiliation: Department of Cellular Membranology, Bogomoletz Institute of Physiology, Kiev 01024, Ukraine; State Key Laboratory for Molecular and Cellular Biology, Kiev 01024, Ukraine.
authors.forename: Oleksandr
authors.initials: O
authors.lastname: Maximyuk
authors.affiliation: Department of Cellular Membranology, Bogomoletz Institute of Physiology, Kiev 01024, Ukraine.
authors.forename: Gleb
authors.initials: G
authors.lastname: Ivanchick
authors.affiliation: Department of Cellular Membranology, Bogomoletz Institute of Physiology, Kiev 01024, Ukraine.
authors.forename: Vadym
authors.initials: V
authors.lastname: Sydorenko
authors.affiliation: Department of Cellular Membranology, Bogomoletz Institute of Physiology, Kiev 01024, Ukraine; State Key Laboratory for Molecular and Cellular Biology, Kiev 01024, Ukraine.
authors.forename: Oleg
authors.initials: O
authors.lastname: Krishtal
authors.affiliation: Department of Cellular Membranology, Bogomoletz Institute of Physiology, Kiev 01024, Ukraine. Electronic address: olena.isaeva@gmail.com.
authors.forename: Elena
authors.initials: E
authors.lastname: Isaeva
mesh_terms: D000367:Age Factors; D000818:Animals; D056547:CA1 Region, Hippocampal / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009924:Organ Culture Techniques; D051381:Rats; D017208:Rats, Wistar; D015222:Sodium Channels / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D015222:Sodium Channels; D013779:Tetrodotoxin
keywords: Hippocampal CA1 pyramidal neurons; Persistent tetrodotoxin-sensitive sodium current; Postnatal development