title: Sodium selenite treatment restores long-lasting ovarian damage induced by irradiation in rats: impact on oxidative stress and apoptosis.
abstract: The deleterious damage of reproductive function following radiotherapy is of increasing importance. In the present study, we investigated the impact of long-term sodium selenite (SS) treatment on radiotherapy-induced ovarian injury in a rat model. Two-week after radiation exposure vaginal cyclicity was arrested, and serum FSH level was elevated in irradiated female rats. SS significantly ameliorated ovarian and uterine oxidative stress induced by irradiation through decreasing the lipid peroxide level and increasing the glutathione level, and glutathione peroxidase activity. In the presence of SS, ovarian cytochrome c and caspase 3 expressions triggered by radiotherapy were decreased. SS significantly counteracted radiation-induced a widespread loss of ovarian follicles and caused further stimulation of follicular proliferation through enhancing PCNA expression. Despite such alteration in ovarian function, serum estradiol level did not change after irradiation, whereas SS significantly increased it. In conclusion, long-term SS treatment improved reproductive development, which was impaired by radiotherapy.
authors.affiliation: National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt.
authors.forename: Riham Soliman
authors.initials: RS
authors.lastname: Said
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
authors.forename: Amira Mohamed
authors.initials: AM
authors.lastname: Badr
authors.affiliation: National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt.
authors.forename: Ahmed Shafik
authors.initials: AS
authors.lastname: Nada
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt. Electronic address: ebtehal_dm@yahoo.com.
authors.forename: Ebtehal
authors.initials: E
authors.lastname: El-Demerdash
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D004958:Estradiol / Q000097:blood; D005260:Female; D005298:Fertility / Q000187:drug effects / Q000528:radiation effects; D005640:Follicle Stimulating Hormone / Q000097:blood; D010053:Ovary / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018038:Sodium Selenite / Q000494:pharmacology*; D014916:Whole-Body Irradiation
chemical_list: D011837:Radiation-Protective Agents; D004958:Estradiol; D005640:Follicle Stimulating Hormone; D018038:Sodium Selenite
keywords: Apoptosis; Folliculogenesis; Irradiation; Oxidative stress; Proliferation; Selenite