title: Expression of CYP2A6, CYP2D6 and CYP4A11 Polymorphisms in COS7 Mammalian Cell Line.
abstract: The cytochrome P450 (P450, CYP) are the superfamily of heme-containing monooxygenase enzymes, found throughout all nature including mammals, plants, and microorganisms. Mammalian P450 enzymes are involved in oxidative metabolism of a wide range of endo- and exogenous chemicals. Especially P450s involved in drug metabolisms are important for drug efficacy and polymorphisms of P450s in individuals reflect differences of drug responses between people. To study the functional differences of CYP2A6, CYP2D6, and CYP4A11 variants, we cloned the four CYP2A6, three CYP2D6, and three CYP4A11 variants, which were found in Korean populations, in mammalian expression vector pcDNA by PCR and examined their expressions in COS-7 mammalian cells using immunoblots using P450 specific polyclonal antibodies. Three of four CYP2A6, two of three CYP4A11, and two of three CYP2D6 variants showed expressions in COS-7 cells but the relative levels of expressions are remarkably different in those of each variants. Our findings may help to study and explain the differences between functions of CYP variants and drug responses in Korean populations.
authors.affiliation: College of Pharmacy, Dongguk University, Seoul 100-715.
authors.forename: Hye Ja
authors.initials: HJ
authors.lastname: Lee
authors.forename: Mi Kyung
authors.initials: MK
authors.lastname: Park
authors.forename: Young Ran
authors.initials: YR
authors.lastname: Park
authors.forename: Donghak
authors.initials: D
authors.lastname: Kim
authors.forename: Chul-Ho
authors.initials: CH
authors.lastname: Yun
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Chun
authors.forename: Hee Jung
authors.initials: HJ
authors.lastname: Shin
authors.forename: Han Sung
authors.initials: HS
authors.lastname: Na
authors.forename: Myeon Woo
authors.initials: MW
authors.lastname: Chung
authors.forename: Chang Hoon
authors.initials: CH
authors.lastname: Lee
keywords: COS-7 mammalian cells; CYP2A6; CYP2D6; CYP4A11; Drug metabolism