title: Cloning and characterization of goose interleukin-17A cDNA.
abstract: Interleukin-17 (IL-17 or IL-17A) is a proinflammatory cytokine produced by activated T cells. IL-17A plays important roles in inflammation and host defense. In this study, the cDNA of the goose IL-17A (GoIL-17A) gene was cloned from thymocytes. Recombinant GoIL-17A (rGoIL-17A) was expressed using a baculovirus expression system and then biologically characterized. The complete open reading frame (ORF) of GoIL-17A contains 510 base pairs that encode 169 amino acid residues, including a 29-amino acid signal peptide and a single potential N-linked glycosylation site. This protein has a molecular weight of 18.9kDa. The amino acid sequence showed 95.9%, 84.6%, 45.0% and 38.4% similarity with the corresponding duck, chicken, rat, and human IL-17A sequences, respectively. The six conserved cysteine residues were also observed in GoIL-17A. A recombinant, mature form of GoIL-17A was produced and its biological activities in goose embryonic fibroblasts were investigated. RT-PCR analysis revealed a marked up-regulation of IL-6 and IL-8 mRNA expression in goose embryonic fibroblasts treated with 1-50 μg of rGoIL-17A for 12h. The GoIL-17A gene sequence and the biologically active recombinant protein may be useful for understanding the role of IL-17A in immune regulation.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Shuangshi
authors.initials: S
authors.lastname: Wei
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Mingchun
authors.initials: M
authors.lastname: Gao
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Zhang
authors.affiliation: School of Life Science, Sun Yat-Sen University, Guangdong 510006, PR China.
authors.forename: Yunhui
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: mabolht@163.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Ma
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: jwwang@neau.edu.cn.
authors.forename: Junwei
authors.initials: J
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005777:Geese / Q000235:genetics / Q000276:immunology*; D005786:Gene Expression Regulation / Q000276:immunology*; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D012313:RNA / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D020381:Interleukin-17; D011994:Recombinant Proteins; D012313:RNA
keywords: Biological activity; Characterization; Goose; IL-17A