title: Establishing serological classification tree model in rheumatoid arthritis using combination of MALDI-TOF-MS and magnetic beads.
abstract: To establish a serological classification tree model for rheumatoid arthritis (RA), protein/peptide profiles of serum were detected by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS) combined with weak cationic exchange (WCX) from Cohort 1, including 65 patients with RA and 41 healthy controls (HC). The samples were randomly divided into a training set and a test set. Twenty-four differentially expressed peaks (P < 0.05) were identified in the training set and 4 of them, namely m/z 3,939, 5,906, 8,146, and 8,569 were chosen to set up our model. This model exhibited a sensitivity of 100.0% and a specificity of 96.0% for differentiating RA patients from HC. The test set reproduced these high levels of sensitivity and specificity, which were 100.0 and 81.2%, respectively. Cohort 2, which include 228 RA patients, was used to further verify the classification efficiency of this model. It came out that 97.4% of them were classified as RA by this model. In conclusion, MALDI-TOF-MS combined with WCX magnetic beads was a powerful method for constructing a classification tree model for RA, and the model we established was useful in recognizing RA.
authors.affiliation: Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100730, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Yan
authors.forename: Hu
authors.initials: H
authors.lastname: Chaojun
authors.forename: Deng
authors.initials: D
authors.lastname: Chuiwen
authors.forename: Leng
authors.initials: L
authors.lastname: Xiaomei
authors.forename: Zhang
authors.initials: Z
authors.lastname: Xin
authors.forename: Li
authors.initials: L
authors.lastname: Yongzhe
authors.forename: Zhang
authors.initials: Z
authors.lastname: Fengchun
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000145:classification* / Q000175:diagnosis* / Q000473:pathology; D001798:Blood Proteins / Q000032:analysis* / Q000378:metabolism; D016022:Case-Control Studies; D003663:Decision Trees; D005260:Female; D006801:Humans; D059346:Magnets; D008297:Male; D008875:Middle Aged; D040901:Proteomics / Q000379:methods / Q000706:statistics & numerical data*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D009626:Terminology as Topic
chemical_list: D001798:Blood Proteins