title: Nucleophosmin contributes to the transcriptional activation function of the Epstein-Barr virus EBNA1 protein.
abstract: The Epstein-Barr virus (EBV) EBNA1 protein plays important roles in latent infection, including transcriptional activation of EBV latency genes by binding to the family-of-repeats (FR) element. Through a proteomic approach, we previously identified an interaction between EBNA1 and the histone chaperone nucleophosmin. Here we show that the EBNA1-nucleophosmin interaction is direct and requires the Gly-Arg-rich sequences that contribute to transactivation. Additionally, nucleophosmin is recruited by EBNA1 to the FR element and is required for EBNA1-mediated transcriptional activation.
authors.affiliation: Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Natasha
authors.initials: N
authors.lastname: Malik-Soni
authors.forename: Lori
authors.initials: L
authors.lastname: Frappier
mesh_terms: D019309:Epstein-Barr Virus Nuclear Antigens / Q000235:genetics / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral / Q000235:genetics / Q000502:physiology*; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000378:metabolism*; D047468:Immunoprecipitation; D009687:Nuclear Proteins / Q000378:metabolism*; D000090243:Nucleophosmin; D040901:Proteomics; D015533:Transcriptional Activation / Q000235:genetics / Q000502:physiology*
chemical_list: D019309:Epstein-Barr Virus Nuclear Antigens; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D000090243:Nucleophosmin; C417029:EBV-encoded nuclear antigen 1