title: Nucleoprotein of influenza B virus binds to its type A counterpart and disrupts influenza A viral polymerase complex formation.
abstract: Upon co-infection with influenza B virus (FluB), influenza A virus (FluA) replication is substantially impaired. Previously, we have shown that the nucleoprotein of FluB (BNP) can inhibit FluA polymerase machinery, retarding the growth of FluA. However, the molecular mechanism underlying this inhibitory action awaited further investigation. Here, we provide evidence that BNP hinders the proper formation of FluA polymerase complex by competitively binding to the nucleoprotein of FluA. To exert this inhibitory effect, BNP must be localized in the nucleus. The interaction does not require the presence of the viral RNA but needs an intact BNP RNA-binding motif. The results highlight the novel role of BNP as an anti-influenza A viral agent and provide insights into the mechanism of intertypic interference.
authors.affiliation: Virology and Cell Technology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathumthani, Thailand. Electronic address: peera.jar@biotec.or.th.
authors.forename: Peera
authors.initials: P
authors.lastname: Jaru-ampornpan
authors.affiliation: Virology and Cell Technology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathumthani, Thailand.
authors.forename: Jaraspim
authors.initials: J
authors.lastname: Narkpuk
authors.affiliation: Virology and Cell Technology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathumthani, Thailand.
authors.forename: Asawin
authors.initials: A
authors.lastname: Wanitchang
authors.affiliation: Virology and Cell Technology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathumthani, Thailand. Electronic address: anan.jon@biotec.or.th.
authors.forename: Anan
authors.initials: A
authors.lastname: Jongkaewwattana
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism / Q000821:virology; D060085:Coinfection / Q000378:metabolism / Q000821:virology; D004285:Dogs; D057809:HEK293 Cells; D006801:Humans; D009980:Influenza A virus / Q000502:physiology*; D009981:Influenza B virus / Q000502:physiology*; D007251:Influenza, Human / Q000378:metabolism / Q000821:virology*; D061985:Madin Darby Canine Kidney Cells; D019590:Nucleocapsid Proteins; D012367:RNA, Viral / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism*; D014758:Viral Core Proteins / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; C057027:PB2 protein, Influenzavirus A; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins; D014764:Viral Proteins; C588186:nucleoprotein, influenza B virus; D012324:RNA-Dependent RNA Polymerase
keywords: Gene reassortment; Influenza virus; Intertypic interference; Nucleoprotein