title: Wogonin, a natural and biologically-active flavonoid, influences a murine WEHI-3 leukemia model in vivo through enhancing populations of T- and B-cells.
abstract: Wogonin, a natural and biologically-active flavonoid found in plants, has been reported to exhibit anticancer effects on several cancer cell types. However, there is no available information regarding the responses to wogonin in leukemia mouse models. At concentrations of 10-200 μM, wogonin reduced the percentage of viable WEHI-3 cells in a concentration-dependent manner. In an in vivo study, WEHI-3 cells were intraperitoneally injected into normal BALB/c mice for establishing leukemic BALB/c mice to determine the anti-leukemia activity of wogonin. Wogonin increased the survival rate and the body weight of leukemic mice when compared to vehicle (olive oil)-treated groups. Furthermore, the results also revealed that wogonin increased the percentage of cluster of differentiation-3 CD3 (T-cell marker) and CD19 (B-cell marker) but reduced that of Mac-3 (macrophages) and CD11b (monocytes) cell surface markers in treated mice as compared with the untreated leukemia group. Based on these observations, wogonin might exhibit anti-leukemia effects on murine WEHI-3 cell line-induced leukemia in vivo.
authors.affiliation: China Medical University, No. 91, Hsueh-Shih Road, Taichung 404, Taiwan. Tel: +886 422053366 ext 2161, jgchung@mail.cmu.edu.tw.
authors.forename: Chin-Chung
authors.initials: CC
authors.lastname: Lin
authors.forename: Jen-Jyh
authors.initials: JJ
authors.lastname: Lin
authors.forename: Ping-Ping
authors.initials: PP
authors.lastname: Wu
authors.forename: Chi-Cheng
authors.initials: CC
authors.lastname: Lu
authors.forename: Jo-Hua
authors.initials: JH
authors.lastname: Chiang
authors.forename: Chao-Lin
authors.initials: CL
authors.lastname: Kuo
authors.forename: Bin-Chuan
authors.initials: BC
authors.lastname: Ji
authors.forename: Ming-Huei
authors.initials: MH
authors.lastname: Lee
authors.forename: An-Cheng
authors.initials: AC
authors.lastname: Huang
authors.forename: Jing-Gung
authors.initials: JG
authors.lastname: Chung
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D044950:Flavanones / Q000494:pharmacology* / Q000627:therapeutic use; D007938:Leukemia / Q000188:drug therapy* / Q000378:metabolism; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D015703:Antigens, CD; D000972:Antineoplastic Agents, Phytogenic; D044950:Flavanones; C085514:wogonin
keywords: BALB/c mice; Wogonin; flavonoid; murine WEHI-3 leukemia model