title: Hyaluronic acid graft polymers displaying peptide antigen modulate dendritic cell response in vitro.
abstract: A novel oxime grafting scheme was utilized to conjugate an ICAM-1 ligand (LABL), a cellular antigen ovalbumin (OVA), or both peptides simultaneously to hyaluronic acid (HA). Samples of HA only and the various peptide grafted HA were found to bind to dendritic cells (DCs). HA with grafted LABL and OVA showed the greatest binding to DCs. Dendritic cells treated with HA, HA with grafted LABL, or HA with grafted LABL and OVA significantly suppressed T cell and DC conjugate formation and T cell proliferation and reduced proinflammatory cytokine production compared to untreated cells. These results suggest that HA serves as an effective backbone for multivalent ligand presentation for inhibiting T cell response to antigen presentation. In addition, multivalent display of both antigen and an ICAM-1 inhibitor (LABL) may enhance binding to DCs and could potentially disrupt cellular signaling leading to autoimmunity.
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmacy, Srinakharinwirot University , 63 Mu 7 Rangsit-Nakhonnayok Road, Ongkharak, Nakhonnayok 26120, Thailand.
authors.forename: Chuda
authors.initials: C
authors.lastname: Chittasupho
authors.forename: Joshua
authors.initials: J
authors.lastname: Sestak
authors.forename: Laura
authors.initials: L
authors.lastname: Shannon
authors.forename: Teruna J
authors.initials: TJ
authors.lastname: Siahaan
authors.forename: Charlotte M
authors.initials: CM
authors.lastname: Vines
authors.forename: Cory
authors.initials: C
authors.lastname: Berkland
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006820:Hyaluronic Acid / Q000378:metabolism*; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000737:chemistry* / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism
chemical_list: D016207:Cytokines; D010446:Peptide Fragments; D011108:Polymers; D018799:Intercellular Adhesion Molecule-1; D006820:Hyaluronic Acid; D010047:Ovalbumin