title: Association of oxidative DNA damage, protein oxidation and antioxidant function with oxidative stress induced cellular injury in pre-eclamptic/eclamptic mothers during fetal circulation.
abstract: Pre-eclampsia is a devastating multi system syndrome and a major cause of maternal, fetal, neonatal morbidity and mortality. Pre-eclampsia is associated with oxidative stress in the maternal circulation. To have an insight on the effect of pre-eclampsia/eclampsia on the neonates, the study was made to explore the oxidative status by quantification of byproducts generated during protein oxidation and oxidative DNA damage and deficient antioxidant activity in umbilical cord blood of pre-eclamptic/eclamptic mothers during fetal circulation. Umbilical cord blood during delivery from neonates born to 19 pre-eclamptic mothers, 14 eclamptic mothers and 18 normotensive mothers (uncomplicated pregnancy) as control cases was collected. 8-OHdG (8-hydroxy-2-deoxyguanosine), protein carbonyl, nitrite, catalase, non-enzymatic antioxidants (vitamin A, E, C), total antioxidant status and iron status were determined. Significant elevation in the levels of 8-OHdG, protein carbonyl, nitrite and iron along with decreased levels of catalase, vitamin A, E, C, total antioxidant status were observed in the umbilical cord blood of pre-eclamptic and eclamptic pregnancies. These parameters might be influential variables for the risk of free radical damage in infants born to pre-eclamptic/eclamptic pregnancies. Increased oxidative stress causes oxidation of DNA and protein which alters antioxidant function. Excess iron level and decreased unsaturated iron binding capacity may be the important factor associated with oxidative stress and contribute in the pathogenesis of pre-eclampsia/eclampsia which is reflected in fetal circulation.
authors.affiliation: Department of Biophysics, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Reena
authors.initials: R
authors.lastname: Negi
authors.affiliation: Department of Biophysics, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Deepti
authors.initials: D
authors.lastname: Pande
authors.affiliation: Department of Biophysics, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Kanchan
authors.initials: K
authors.lastname: Karki
authors.affiliation: Department of Pediatrics, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Ashok
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Ranjana S
authors.initials: RS
authors.lastname: Khanna
authors.affiliation: Department of Biophysics, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India. Electronic address: hdkhanna@yahoo.co.in.
authors.forename: Hari D
authors.initials: HD
authors.lastname: Khanna
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D016022:Case-Control Studies; D004247:DNA / Q000235:genetics; D004249:DNA Damage; D004461:Eclampsia / Q000097:blood / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005312:Fetal Blood / Q000378:metabolism* / Q000502:physiology; D005333:Fetus / Q000098:blood supply*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007501:Iron / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D011225:Pre-Eclampsia / Q000097:blood / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: D000975:Antioxidants; D011506:Proteins; D004247:DNA; D007501:Iron
keywords: Antioxidant status; Oxidative DNA/protein damage; Oxidative stress; Pre-eclampsia/eclampsia