title: Elucidating the interacting domains of chandipura virus nucleocapsid protein.
abstract: The nucleocapsid (N) protein of Chandipura virus (CHPV) plays a crucial role in viral life cycle, besides being an important structural component of the virion through proper organization of its interactions with other viral proteins. In a recent study, the authors had mapped the associations among CHPV proteins and shown that N protein interacts with four of the viral proteins: N, phosphoprotein (P), matrix protein (M), and glycoprotein (G). The present study aimed to distinguish the regions of CHPV N protein responsible for its interactions with other viral proteins. In this direction, we have generated the structure of CHPV N protein by homology modeling using SWISS-MODEL workspace and Accelrys Discovery Studio client 2.55 and mapped the domains of N protein using PiSQRD. The interactions of N protein fragments with other proteins were determined by ZDOCK rigid-body docking method and validated by yeast two-hybrid and ELISA. The study revealed a unique binding site, comprising of amino acids 1-30 at the N terminus of the nucleocapsid protein (N1) that is instrumental in its interactions with N, P, M, and G proteins. It was also observed that N2 associates with N and G proteins while N3 interacts with N, P, and M proteins.
authors.affiliation: Center for Emerging Diseases, Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector 62, Noida, Uttar Pradesh 201 307, India.
authors.forename: Kapila
authors.initials: K
authors.lastname: Kumar
authors.forename: Sreejith
authors.initials: S
authors.lastname: Rajasekharan
authors.forename: Sahil
authors.initials: S
authors.lastname: Gulati
authors.forename: Jyoti
authors.initials: J
authors.lastname: Rana
authors.forename: Reema
authors.initials: R
authors.lastname: Gabrani
authors.forename: Chakresh K
authors.initials: CK
authors.lastname: Jain
authors.forename: Amita
authors.initials: A
authors.lastname: Gupta
authors.forename: Vijay K
authors.initials: VK
authors.lastname: Chaudhary
authors.forename: Sanjay
authors.initials: S
authors.lastname: Gupta