title: Using coculture to detect chemically mediated interspecies interactions.
abstract: In nature, bacteria rarely exist in isolation; they are instead surrounded by a diverse array of other microorganisms that alter the local environment by secreting metabolites. These metabolites have the potential to modulate the physiology and differentiation of their microbial neighbors and are likely important factors in the establishment and maintenance of complex microbial communities. We have developed a fluorescence-based coculture screen to identify such chemically mediated microbial interactions. The screen involves combining a fluorescent transcriptional reporter strain with environmental microbes on solid media and allowing the colonies to grow in coculture. The fluorescent transcriptional reporter is designed so that the chosen bacterial strain fluoresces when it is expressing a particular phenotype of interest (i.e. biofilm formation, sporulation, virulence factor production, etc.) Screening is performed under growth conditions where this phenotype is not expressed (and therefore the reporter strain is typically nonfluorescent). When an environmental microbe secretes a metabolite that activates this phenotype, it diffuses through the agar and activates the fluorescent reporter construct. This allows the inducing-metabolite-producing microbe to be detected: they are the nonfluorescent colonies most proximal to the fluorescent colonies. Thus, this screen allows the identification of environmental microbes that produce diffusible metabolites that activate a particular physiological response in a reporter strain. This publication discusses how to: a) select appropriate coculture screening conditions, b) prepare the reporter and environmental microbes for screening, c) perform the coculture screen, d) isolate putative inducing organisms, and e) confirm their activity in a secondary screen. We developed this method to screen for soil organisms that activate biofilm matrix-production in Bacillus subtilis; however, we also discuss considerations for applying this approach to other genetically tractable bacteria.
authors.affiliation: Department of Biology, University of North Carolina at Chapel Hill.
authors.forename: Elizabeth Anne
authors.initials: EA
authors.lastname: Shank
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D018407:Bacterial Physiological Phenomena; D001431:Bacteriological Techniques / Q000379:methods*; D018441:Biofilms; D018920:Coculture Techniques / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D017930:Genes, Reporter; D011401:Promoter Regions, Genetic; D012988:Soil Microbiology; D014158:Transcription, Genetic
chemical_list: D005456:Fluorescent Dyes