title: Interplay of the fungal sumoylation network for control of multicellular development.
abstract: The role of the complex network of the ubiquitin-like modifier SumO in fungal development was analysed. SumO is not only required for sexual development but also for accurate induction and light stimulation of asexual development. The Aspergillus nidulans COMPASS complex including its subunits CclA and the methyltransferase SetA connects the SumO network to histone modification. SetA is required for correct positioning of aerial hyphae for conidiophore and asexual spore formation. Multicellular fungal development requires sumoylation and desumoylation. This includes the SumO processing enzyme UlpB, the E1 SumO activating enzyme AosA/UbaB, the E2 conjugation enzyme UbcN and UlpA as major SumO isopeptidase. Genetic suppression analysis suggests a connection between the genes for the Nedd8 isopeptidase DenA and the SumO isopeptidase UlpA and therefore a developmental interplay between neddylation and sumoylation in fungi. Biochemical evidence suggests an additional connection of the fungal SumO network with ubiquitination. Members of the cellular SumO network include histone modifiers, components of the transcription, RNA maturation and stress response machinery, or metabolic enzymes. Our data suggest that the SumO network controls specific temporal and spatial steps in fungal differentiation.
authors.affiliation: Institut für Mikrobiologie und Genetik, Georg-August Universität Göttingen, Grisebachstrasse 8, D-37077, Göttingen, Germany.
authors.forename: Rebekka
authors.initials: R
authors.lastname: Harting
authors.forename: Ozgür
authors.initials: O
authors.lastname: Bayram
authors.forename: Karen
authors.initials: K
authors.lastname: Laubinger
authors.forename: Oliver
authors.initials: O
authors.lastname: Valerius
authors.forename: Gerhard H
authors.initials: GH
authors.lastname: Braus
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D002352:Carrier Proteins; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005800:Genes, Fungal; D025301:Hyphae / Q000378:metabolism; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D025841:Small Ubiquitin-Related Modifier Proteins / Q000235:genetics / Q000378:metabolism*; D058207:Sumoylation; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D044763:Ubiquitin-Conjugating Enzymes / Q000378:metabolism; D014452:Ubiquitins / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D005656:Fungal Proteins; C583727:SIMC1 protein, human; D025841:Small Ubiquitin-Related Modifier Proteins; D014157:Transcription Factors; D014452:Ubiquitins; D008780:Methyltransferases; D044763:Ubiquitin-Conjugating Enzymes; D010447:Peptide Hydrolases