title: Influence of lysophosphatidic acid on estradiol production and follicle stimulating hormone action in bovine granulosa cells.
abstract: The objective of the study was to examine the effect of lysophosphatidic acid (LPA) on 17β-estradiol (E2) synthesis and follicle stimulating hormone (FSH) action in bovine granulosa cells. We found that granulosa cells in the bovine antral follicle, in addition to the uterus and the CL, are also the site of LPA synthesis and the target for LPA action in the bovine reproductive tract. Our findings suggest that LPA stimulates E2 synthesis, probably via increased expression of FSHR and 17β-HSD genes.
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Boruszewska
authors.forename: Emilia
authors.initials: E
authors.lastname: Sinderewicz
authors.forename: Ilona
authors.initials: I
authors.lastname: Kowalczyk-Zieba
authors.forename: Dariusz Jan
authors.initials: DJ
authors.lastname: Skarzynski
authors.forename: Izabela
authors.initials: I
authors.lastname: Woclawek-Potocka
mesh_terms: D000818:Animals; D002417:Cattle; D003338:Corpus Luteum / Q000187:drug effects / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D004958:Estradiol / Q000096:biosynthesis*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D008246:Lysophospholipids / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D014599:Uterus / Q000187:drug effects / Q000378:metabolism
chemical_list: D017931:DNA Primers; D008246:Lysophospholipids; D004958:Estradiol; D005640:Follicle Stimulating Hormone; C032881:lysophosphatidic acid
keywords: Autotaxin; Cow; Estradiol; Follicle stimulating hormone; Granulosa cells; LPAR; Lysophosphatidic acid; Phospholipase A2