title: Effect of the co-occurring olive oil and thyme extracts on the phenolic bioaccessibility and bioavailability assessed by in vitro digestion and cell models.
abstract: Olive oils flavoured with edible herbs have grown in popularity because of their added value and potential health benefits. However, the combined presence of different phytochemicals from olive oil and herbs requires study of their possible interactions during intestinal transport and metabolism. The aim of this study was firstly to evaluate the effect on bioaccessibility of the co-occurring bioactive compounds from olive oil and thyme through an in vitro digestion model of three extracts: olive extract (OE), thyme extract (TE) and a combination of both (OTE). The bioaccessible fractions were exposed to Caco-2 and HepG-2 cell models, as well as to a co-culture of both of these. Results indicated that the bioaccessibility of hydroxytyrosol was enhanced when OTE was digested. After Caco-2 cells exposure, no significant differences were observed in hydroxytyrosol transport, whereas the main flavonoids from thyme seemed to undergo an enhanced basolateral permeation when both phenolic sources where exposed.
authors.affiliation: Department of Food Technology, XeRTA-UTPV, Escola Tècnica Superior d'Enginyeria Agrària, Universitat de Lleida, Avda/Alcalde Rovira Roure 191, 25198 Lleida, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Rubió
authors.forename: Alba
authors.initials: A
authors.lastname: Macià
authors.forename: Anna
authors.initials: A
authors.lastname: Castell-Auví
authors.forename: Montserrat
authors.initials: M
authors.lastname: Pinent
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Blay
authors.forename: Anna
authors.initials: A
authors.lastname: Ardévol
authors.forename: Maria-Paz
authors.initials: MP
authors.lastname: Romero
authors.forename: Maria-José
authors.initials: MJ
authors.lastname: Motilva
mesh_terms: D001682:Biological Availability; D018938:Caco-2 Cells; D004063:Digestion; D005419:Flavonoids / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D008954:Models, Biological; D031658:Olea / Q000737:chemistry / Q000378:metabolism; D000069463:Olive Oil; D010636:Phenols / Q000378:metabolism*; D010936:Plant Extracts / Q000378:metabolism*; D010938:Plant Oils / Q000378:metabolism*; D046930:Thymus Plant / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005419:Flavonoids; D000069463:Olive Oil; D010636:Phenols; D010936:Plant Extracts; D010938:Plant Oils
keywords: Caco-2; HepG-2; Metabolism; Olive phenols; Thyme; Transport