title: Effect of outdoor conditions on Nannochloropsis salina cultivation in artificial seawater using nutrients from anaerobic digestion effluent.
abstract: The effects of simulated outdoor seasonal climatic conditions on Nannochloropsis salina (N. salina) grown using nutrients from anaerobic digestion (AD) effluent were evaluated in this study. Under various light exposure (LE) and temperature (10-30 °C) conditions, N. salina specific growth rate (μ) was strongly affected by LE. Light availability (LA) was observed to be crucial for biomass production, with μ values of 0.038±0.013 d(-1), 0.093±0.013 d(-1), and 0.151±0.021 d(-1) for 6-h, 12-h, and 24-h LA conditions, respectively. Temperature (10-25 °C) was not significant in affecting the light dependent growth coefficient (μ/LE), indicating the suitability of culturing this strain in the Ohio climate. Cultures exposed to low illumination had significantly higher unsaturated fatty acid content than those under high illumination, with nearly 29% higher eicosapentaenoic acid (C20:5) content. Using LE and light attenuation resulted in adequate prediction of N. salina growth in a 1000 L open raceway pond.
authors.affiliation: Department of Food, Agricultural and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH 44691-4096, USA.
authors.forename: Johnathon P
authors.initials: JP
authors.lastname: Sheets
authors.affiliation: Department of Food, Agricultural and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH 44691-4096, USA.
authors.forename: Xumeng
authors.initials: X
authors.lastname: Ge
authors.affiliation: Department of Food, Agricultural and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH 44691-4096, USA.
authors.forename: Stephen Y
authors.initials: SY
authors.lastname: Park
authors.affiliation: Department of Food, Agricultural and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH 44691-4096, USA. Electronic address: li.851@osu.edu.
authors.forename: Yebo
authors.initials: Y
authors.lastname: Li
mesh_terms: D000693:Anaerobiosis / Q000187:drug effects / Q000528:radiation effects; D019149:Bioreactors / Q000382:microbiology; D003198:Computer Simulation; D005227:Fatty Acids / Q000096:biosynthesis; D007700:Kinetics; D008027:Light; D058086:Microalgae / Q000187:drug effects / Q000254:growth & development* / Q000528:radiation effects; D008962:Models, Theoretical; D009584:Nitrogen / Q000032:analysis / Q000494:pharmacology*; D010758:Phosphorus / Q000032:analysis / Q000494:pharmacology*; D061005:Ponds; D015203:Reproducibility of Results; D012621:Seasons; D012623:Seawater / Q000382:microbiology*; D013696:Temperature; D014865:Waste Disposal, Fluid
chemical_list: D005227:Fatty Acids; D010758:Phosphorus; D009584:Nitrogen
keywords: Anaerobic digestion effluent; Biodiesel; Lipids; Microalgae; Nannochloropsis salina