title: Enhancement of bioelectricity generation by manipulation of the electron shuttles synthesis pathway in microbial fuel cells.
abstract: Microbial fuel cells (MFCs) are promising for generating bioenergy and treating organic waste simultaneously. However, low extracellular electron transfer (EET) efficiency between electrogens and anodes remains one of the major bottlenecks in practical applications of MFCs. In this paper, pyocyanin (PYO) synthesis pathway was manipulated to improve the EET efficiency in Pseudomonas aeruginosa-inoculated MFCs. By overexpression of phzM (methyltransferase encoding gene), the maximum power density of P. aeruginosa-phzM-inoculated MFC was enhanced to 166.68 μW/cm(2), which was four folds of the original strain. In addition, the phzM overexpression strain exhibited an increase of 1.6 folds in PYO production and about a onefold decrease in the total internal resistance than the original strain, which should underlie the enhancement of the EET efficiency and the electricity power output (EPT). On the basis of these results, the manipulation of electron shuttles synthesis pathways could be an efficient approach to improve the EPT of MFCs.
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210095, China; Bioenergy Research Institute, Nanjing University of Technology, Nanjing 210095, China. Electronic address: yongxiaoyu@njut.edu.cn.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Yong
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210095, China.
authors.forename: Dong-Yan
authors.initials: DY
authors.lastname: Shi
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210095, China.
authors.forename: Yi-Lu
authors.initials: YL
authors.lastname: Chen
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210095, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Feng
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210095, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210095, China; Bioenergy Research Institute, Nanjing University of Technology, Nanjing 210095, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210095, China; Bioenergy Research Institute, Nanjing University of Technology, Nanjing 210095, China.
authors.forename: Shu-Ya
authors.initials: SY
authors.lastname: Wang
authors.affiliation: Biofuels Institute, School of the Environment, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Yang-Chun
authors.initials: YC
authors.lastname: Yong
authors.affiliation: Guangzhou Institute of Energy Conversion, Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, China.
authors.forename: Yong-Ming
authors.initials: YM
authors.lastname: Sun
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210095, China; Bioenergy Research Institute, Nanjing University of Technology, Nanjing 210095, China.
authors.forename: Ping-Kai
authors.initials: PK
authors.lastname: OuYang
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210095, China; Bioenergy Research Institute, Nanjing University of Technology, Nanjing 210095, China. Electronic address: hellozheng@gmail.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Zheng
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001674:Bioelectric Energy Sources; D004560:Electricity; D055664:Electrochemical Techniques; D004579:Electron Transport; D004583:Electrons; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000378:metabolism; D011710:Pyocyanine / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D011710:Pyocyanine
keywords: Electron shuttles; Microbial fuel cells; Pseudomonas aeruginosa; Pyocyanin (PYO); phzM