title: Streptomyces tunisiensis sp. nov., a novel Streptomyces species with antibacterial activity.
abstract: A novel actinomycete strain designated CN-207(T) was isolated from northern Tunisian soil. This strain exhibited potent broad spectrum antibacterial activity against clinical isolates of methicillin-resistant Staphylococcus species and several other Gram-positive and Gram-negative bacteria. Strain CN-207(T) developed greyish aerial mycelium and pale grey substrate mycelium on yeast extract/malt agar. The isolate produced branching vegetative mycelia with sporangiophores bearing sporangia developing at a late stage of growth. The sporangia contained smooth, non-motile spores. Chemotaxonomic characteristics of strain CN-207(T) were typical of the Streptomyces genus. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CN-207(T) belonged to the genus Streptomyces, and was most closely related to Streptomyces griseoincarnatus DSM 40274(T), Streptomyces variabilis DSM 40179(T), Streptomyces labedae DSM 41446(T) and Streptomyces erythrogriseus DSM 40116(T). Low DNA-DNA relatedness values were recorded between strain CN-207(T) and its closest phylogenetic neighbours. Strain CN-207(T) was also distinguished from the nearest phylogenetic neighbours using a combination of morphological and phenotypic characteristics. On the basis of its phenotypic and molecular properties, strain CN-207(T) is considered as a novel species of the Streptomyces genus, for which the name Streptomyces tunisiensis sp. nov. is proposed. The type strain is CN-207(T) (=JCM 17589(T) = DSM 42037(T)).
authors.affiliation: Laboratoire des Substances Bioactives, Centre de Biotechnologie de Borj Cedria, 901, 2050, Hammam-lif, Tunisia.
authors.forename: Nedra
authors.initials: N
authors.lastname: Slama
authors.forename: Houda
authors.initials: H
authors.lastname: Mankai
authors.forename: Ameni
authors.initials: A
authors.lastname: Ayed
authors.forename: Karim
authors.initials: K
authors.lastname: Mezhoud
authors.forename: Catherine
authors.initials: C
authors.lastname: Rauch
authors.forename: Hadeer
authors.initials: H
authors.lastname: Lazim
authors.forename: Insaf
authors.initials: I
authors.lastname: Barkallah
authors.forename: Maher
authors.initials: M
authors.lastname: Gtari
authors.forename: Ferid
authors.initials: F
authors.lastname: Limam
mesh_terms: D000898:Antibiosis; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D055624:Methicillin-Resistant Staphylococcus aureus / Q000254:growth & development*; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013171:Spores, Bacterial / Q000166:cytology; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D014416:Tunisia
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S