title: MMP-2, -9 and TIMP-1, -2 assays in keratinocyte cultures.
abstract: To determine the status of tissue metabolism in the epidermis, matrix metalloproteinase (MMP)-2 and -9 and tissue inhibitor of metalloproteinases (TIMP)-1 and -2 can be detected using keratinocytes in culture. In addition to Western blotting analysis, gelatin zymography for MMP-2 and -9 and the reverse zymography for TIMP-1 and -2 are useful methods for evaluating such protein expressions both qualitatively and quantitatively, because MMP-2 and MMP-9 are known as gelatinase. Moreover, real-time analysis for zymography can be performed using fluorescein isothiocyanate-labelled gelatin.
authors.affiliation: Department of Dermatology, Teikyo University Chiba Medical Center, Ichihara, Japan, tkobayas@med.teikyo-u.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kobayashi
mesh_terms: D015153:Blotting, Western; D002478:Cells, Cultured; D004586:Electrophoresis; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000302:isolation & purification / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000302:isolation & purification / Q000378:metabolism*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9