title: Medical-grade collagen peptide in injectables provides antioxidant protection.
abstract: Medical-grade collagen peptide is used as an additive agent in pharmaceutical formulations; however, it is unknown as to whether the compound exerts antioxidant effects in vitro. In this study, we evaluated the antioxidant effects of medical-grade collagen peptide on reactive oxygen species such as hydroxyl radical, superoxide anion radical and singlet oxygen using electron spin resonance and spin trapping. We confirmed that medical-grade collagen peptide directly inhibited hydroxyl radical generated by the Fenton reaction or by ultraviolet irradiation of hydrogen peroxide, and singlet oxygen. In addition, an antioxidant effect of medical-grade collagen peptide on singlet oxygen was observed in peptide fractions 12-22. The total amount of antioxidant amino acids (Gly, Hyp, Glu, Ala, Cys, Met and His) constituted more than half of the total amino acids in these fractions. These results suggest that the observed antioxidant properties of medical-grade collagen peptide are due to the compound containing antioxidant amino acids. Medical-grade collagen peptide, which is used in pharmaceuticals, and especially in injectables, could provide useful antioxidant properties to protect the active ingredient from oxidation.
authors.affiliation: Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College , 82 Inaoka-cho, Yokosuka, Kanagawa , Japan .
authors.forename: Kyo
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Yojiro
authors.initials: Y
authors.lastname: Maehata
authors.forename: Yasue
authors.initials: Y
authors.lastname: Okada
authors.forename: Masashi
authors.initials: M
authors.lastname: Kusubata
authors.forename: Shunji
authors.initials: S
authors.lastname: Hattori
authors.forename: Keisuke
authors.initials: K
authors.lastname: Tanaka
authors.forename: Chihiro
authors.initials: C
authors.lastname: Miyamoto
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Yoshino
authors.forename: Ayaka
authors.initials: A
authors.lastname: Yoshida
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Tokutomi
authors.forename: Satoko
authors.initials: S
authors.lastname: Wada-Takahashi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Komatsu
authors.forename: Takero
authors.initials: T
authors.lastname: Otsuka
authors.forename: Shun-Suke
authors.initials: SS
authors.lastname: Takahashi
authors.forename: Masaichi-Chang-Il
authors.initials: MC
authors.lastname: Lee
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D003094:Collagen / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D006861:Hydrogen Peroxide / Q000737:chemistry; D017665:Hydroxyl Radical / Q000737:chemistry; D007267:Injections; D007501:Iron / Q000737:chemistry; D010446:Peptide Fragments / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D011310:Preservatives, Pharmaceutical / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D026082:Singlet Oxygen / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry
chemical_list: D000596:Amino Acids; D000975:Antioxidants; C045076:Fenton's reagent; D010446:Peptide Fragments; D011310:Preservatives, Pharmaceutical; D013481:Superoxides; D026082:Singlet Oxygen; D017665:Hydroxyl Radical; D003094:Collagen; D006861:Hydrogen Peroxide; D007501:Iron
keywords: Antioxidant property; electron spin resonance; reactive oxygen species