title: Impact of biochemical design on estrogen receptor/estrogen response element interaction by surface plasmon resonance technology.
abstract: The estrogen receptor (ER) is a transcription factor that binds under 17-β-estradiol (E2) stimulation as homodimer to a short DNA consensus sequence named estrogen response element (ERE). The ER/ERE interaction has been assessed by several research groups through different methodologies notably by surface plasmon resonance (SPR) techniques. The biochemical parameters and conditions (solvent, ER concentration, salt, time and temperature) used to prepare samples before analysis were very different from one study to another. But no studies have aimed to compare the effect of these modifications on ER/ERE interaction. Therefore the main objective of the present paper was to assess the influence of biochemical parameters onto the ER/ERE interaction with the final aim to improve the comprehension of this interaction. Our results highlighted that parameters like solvent, ER concentration, salt and surfactant concentration, temperature and time deeply modify ER/ERE interaction. Nevertheless, the dimer formation under E2 stimulation occurred with all tested conditions. Altogether, incubation parameters of ER with E2, deeply modify its binding level onto ERE. These data constitute an important key point to consider for the improvement of ER/ERE detection method depending upon the aim of the study (interaction measurement, environmental detection, development of new technologies or devices).
authors.affiliation: Laboratoire Génie de l'Environnement Industriel, Ecole des Mines d'Alès, 6 Avenue de Clavières, 30319 Alès Cedex, France; INSERM U1085, Institut de Recherche en Santé, Environnement et Travail (IRSET), Equipe "TREC", Université de Rennes 1, SFR Biosit, Campus de Beaulieu, Bat 13, 263 avenue du Général Leclerc, CS 74205, 35042 RENNES cedex, France. Electronic address: denis.habauzit@univ-rennes1.fr.
authors.forename: Denis
authors.initials: D
authors.lastname: Habauzit
authors.affiliation: Laboratoire Génie de l'Environnement Industriel, Ecole des Mines d'Alès, 6 Avenue de Clavières, 30319 Alès Cedex, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Bayle
authors.affiliation: Université de Nîmes, Rue Georges Salan, 30021 Nîmes, France.
authors.forename: David
authors.initials: D
authors.lastname: Benimelis
authors.affiliation: Université de Nîmes, Rue Georges Salan, 30021 Nîmes, France; Institut Charles Gerhardt Montpellier, UMR 5253 CNRS-ENSCM UM2-UM1, Ecole Nationale Supérieure de Chimie, Montpellier, France.
authors.forename: Joel
authors.initials: J
authors.lastname: Chopineau
authors.affiliation: Université de Nîmes, Rue Georges Salan, 30021 Nîmes, France; EHESP Rennes, Sorbonne Paris Cité, Avenue du Professeur Léon Bernard, CS 74312, 35043 Rennes Cedex, France; INSERM U1085-IRSET, LERES, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Roig
mesh_terms: D002021:Buffers; D003902:Detergents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism*; D007700:Kinetics; D055503:Protein Multimerization / Q000187:drug effects; D055550:Protein Stability / Q000187:drug effects; D020836:Protein Structure, Quaternary; D011960:Receptors, Estrogen / Q000737:chemistry / Q000378:metabolism*; D020218:Response Elements; D012492:Salts / Q000494:pharmacology; D012997:Solvents / Q000737:chemistry; D020349:Surface Plasmon Resonance; D013696:Temperature
chemical_list: D002021:Buffers; D003902:Detergents; D004967:Estrogens; D011960:Receptors, Estrogen; D012492:Salts; D012997:Solvents; D004958:Estradiol
keywords: Dimerization; Estrogen; Estrogen receptor; Solvent; Surface plasmon resonance; Temperature