title: Evolution of plant eukaryotic initiation factor 4E (eIF4E) and potyvirus genome-linked protein (VPg): a game of mirrors impacting resistance spectrum and durability.
abstract: Polymorphism in the plant eukaryotic translation initiation factor 4E (eIF4E) and potyvirus genome-linked protein (VPg) determine, in many cases, the outcome of the confrontation between these two organisms: compatibility (i.e. infection of the plant by the virus) or incompatibility (i.e. resistance of the plant to the virus). The two interacting proteins eIF4E and VPg show strikingly similar evolution patterns. Most codon positions in their coding sequences are highly constrained for nonsynonymous substitutions but a small number shows evidence for positive selection. Several of these latter positions were shown to be functionally important, conferring resistance to the host or pathogenicity to the virus. Determining the mutational pathways involved in pepper eIF4E diversification revealed a link between an increase of the pepper resistance spectrum towards a panel of potyvirus species and an increase of durability of the resistance towards Potato virus Y. This relationship questions the interest of using more generally the spectrum of action of a plant resistance gene as a predictor of its durability potential.
authors.affiliation: INRA, UR407 Pathologie Végétale, Domaine Saint Maurice, CS 60094, F-84143 Montfavet Cedex, France. Electronic address: moury@avignon.inra.fr.
authors.forename: B
authors.initials: B
authors.lastname: Moury
authors.affiliation: INRA, UR1052, Génétique et Amélioration des Fruits et Légumes, Domaine Saint Maurice, CS 60094, F-84143 Montfavet Cedex, France.
authors.forename: C
authors.initials: C
authors.lastname: Charron
authors.affiliation: INRA, UR407 Pathologie Végétale, Domaine Saint Maurice, CS 60094, F-84143 Montfavet Cedex, France; INRA, UR1052, Génétique et Amélioration des Fruits et Légumes, Domaine Saint Maurice, CS 60094, F-84143 Montfavet Cedex, France.
authors.forename: B
authors.initials: B
authors.lastname: Janzac
authors.affiliation: INRA, UR407 Pathologie Végétale, Domaine Saint Maurice, CS 60094, F-84143 Montfavet Cedex, France.
authors.forename: V
authors.initials: V
authors.lastname: Simon
authors.affiliation: INRA, UR1052, Génétique et Amélioration des Fruits et Légumes, Domaine Saint Maurice, CS 60094, F-84143 Montfavet Cedex, France.
authors.forename: J L
authors.initials: JL
authors.lastname: Gallois
authors.affiliation: INRA, UR1052, Génétique et Amélioration des Fruits et Légumes, Domaine Saint Maurice, CS 60094, F-84143 Montfavet Cedex, France.
authors.forename: A
authors.initials: A
authors.lastname: Palloix
authors.affiliation: INRA, UR1052, Génétique et Amélioration des Fruits et Légumes, Domaine Saint Maurice, CS 60094, F-84143 Montfavet Cedex, France.
authors.forename: C
authors.initials: C
authors.lastname: Caranta
mesh_terms: D016000:Cluster Analysis; D003062:Codon; D060467:Disease Resistance / Q000235:genetics; D039561:Eukaryotic Initiation Factor-4E / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019143:Evolution, Molecular; D006239:Haplotypes; D054884:Host-Pathogen Interactions; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D010935:Plant Diseases; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010944:Plants / Q000378:metabolism* / Q000821:virology; D017800:Potyvirus / Q000378:metabolism*; D012641:Selection, Genetic; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D003062:Codon; D039561:Eukaryotic Initiation Factor-4E; D010940:Plant Proteins; D014764:Viral Proteins
keywords: Capsicum annuum; Host resistance; Host–parasite coevolution; Potyvirus; VPg; eIF4E