title: [Fluorescence in situ hybridization on histologic sections].
abstract: I-FISH (fluorescence in situ hybridization on interphasic nuclei) represents a laboratory method linking morphological investigations (histological sections of formaldehyde fixed and paraffin embedded tissues) with molecular techniques (sequence specificity of nucleic acids bases for a certain locus). I-FISH is relatively undemanding for a laboratory workout, but offering a lot of important information about the investigated cells. Within a scope of pathology departments I-FISH is utilized mostly in diagnostics of neoplasms. I-FISH is helpful in detecting gene copy numbers (amplifications or deletions), and, importantly, in establishing copy numbers of individual chromosomes (polysomies or monosomies), chromosomal breaks and translocations. At present, I-FISH is used not only for diagnosis and estimation of prognosis, but also as a method to qualify a patient for a targeted biological therapy. Because demands on investigation of solid tumors keep raising I-FISH becomes a part of routine investigations. The aim of this paper is to summarize principles and the utility of I-FISH and to help the interested readers in finding a basic orientation in this laboratory method.
authors.forename: Marcela
authors.initials: M
authors.lastname: Mrhalová
authors.forename: Roman
authors.initials: R
authors.lastname: Kodet
mesh_terms: D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D009369:Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D057089:Pathology, Molecular / Q000379:methods*; D011379:Prognosis