title: Transcriptome profiling of brain edemas caused by influenza infection and lipopolysaccharide treatment.
abstract: Influenza A virus-associated encephalopathy triggered by influenza virus infection often occurs in children aged five and younger in Japan. However, the mechanisms behind Influenza A virus-associated encephalopathy are not yet well understood. This study developed an Influenza A virus-associated encephalopathy-like model using mice infected with Influenza A virus and given lipopolysaccharide treatment. The results showed that the mice used in the model suffered from brain edemas nearly three times more severe, as well as having higher cytokine levels in sera compared to those of the control groups. Using gene expression profiling, cytokine-related genes were found not to be up-regulated in the brain in situ, while protein coding genes, which are known to be involved in blood-brain barrier disruption, were up-regulated. Categorizing the functional groups using gene ontology revealed the terms "ion channels," "calcium oscillation," and "membrane transporter activities." The blood-brain barrier disruption found in this Influenza A virus-associated encephalopathy model can therefore be assumed to be due to a cellular electrolyte imbalance of the neuronal tissue, in addition to a cytokine storm.
authors.affiliation: Graduate School of Medicine, Kobe University, Hyogo, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Kyan
authors.forename: Yumi
authors.initials: Y
authors.lastname: Ueda
authors.forename: Mitsutaka
authors.initials: M
authors.lastname: Yoshida
authors.forename: Kenji
authors.initials: K
authors.lastname: Sasahara
authors.forename: Kyoko
authors.initials: K
authors.lastname: Shinya
mesh_terms: D000818:Animals; D001929:Brain Edema / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D020869:Gene Expression Profiling; D008070:Lipopolysaccharides / Q000633:toxicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000473:pathology*
chemical_list: D008070:Lipopolysaccharides
keywords: CNS; cytokine storm; electrolyte deficiency; encephalopathy; influenza virus; microarray