title: Evaluation of nestin in lung adenocarcinoma: relation to VEGF and Bcl-2.
abstract: BACKGROUND
  Nestin is a marker of multipotent precursor cells that is up regulated in cancer.
  
  
  OBJECTIVE
  To explore its diagnostic role and its relationship to vascular endothelial growth factor (VEGF) and Bcl-2 in lung adenocarcinoma.
  
  
  METHODS
  Evaluation of nestin expression in lung biopsies by real-time PCR and serum VEGF and Bcl-2 by ELISA in 27 adenocarcinoma patients and 15 control subjects.
  
  
  RESULTS
  Nestin was significantly higher in lung adenocarcinoma patients especially with advanced grade and stage and it was significantly correlated to VEGF and Bcl-2.
  
  
  CONCLUSIONS
  Nestin can be considered as a potential diagnostic marker in lung adenocarcinoma.
authors.affiliation: Department of Medical Biochemistry and.
authors.forename: Manal Basyouni
authors.initials: MB
authors.lastname: Ahmed
authors.forename: Enas Samir
authors.initials: ES
authors.lastname: Nabih
authors.forename: Manal Louis
authors.initials: ML
authors.lastname: Louka
authors.forename: Fayda Ibrahim
authors.initials: FI
authors.lastname: Abdel Motaleb
authors.forename: Mohamed Ali
authors.initials: MA
authors.lastname: El Sayed
authors.forename: Hisham Mahmoud
authors.initials: HM
authors.lastname: Elwakiel
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D015870:Gene Expression; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D064231:Nestin / Q000235:genetics / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000097:blood*; D012372:ROC Curve; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*
chemical_list: D014408:Biomarkers, Tumor; C577484:NES protein, human; D064231:Nestin; D019253:Proto-Oncogene Proteins c-bcl-2; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A