title: Paper-based device for rapid typing of secondary human blood groups.
abstract: We report the use of bioactive paper for typing of secondary human blood groups. Our recent work on using bioactive paper for human blood typing has led to the discovery of a new method for identifying haemagglutination of red blood cells. The primary human blood groups, i.e., ABO and RhD groups, have been successfully typed with this method. Clinically, however, many secondary blood groups can also cause fatal blood transfusion accidents, despite the fact that the haemagglutination reactions of secondary blood groups are generally weaker than those of the primary blood groups. We describe the design of a user-friendly sensor for rapid typing of secondary blood groups using bioactive paper. We also present mechanistic insights into interactions between secondary blood group antibodies and red blood cells obtained using confocal microscopy. Haemagglutination patterns under different conditions are revealed for optimization of the assay conditions.
authors.affiliation: Department of Chemical Engineering, Monash University, Wellington Rd, Clayton, VIC, 3800, Australia.
authors.forename: Miaosi
authors.initials: M
authors.lastname: Li
authors.forename: Whui Lyn
authors.initials: WL
authors.lastname: Then
authors.forename: Lizi
authors.initials: L
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
mesh_terms: D001681:Biological Assay / Q000191:economics / Q000295:instrumentation; D001774:Blood Chemical Analysis / Q000191:economics / Q000295:instrumentation*; D001789:Blood Group Antigens / Q000032:analysis*; D001788:Blood Grouping and Crossmatching / Q000191:economics / Q000295:instrumentation*; D006801:Humans; D057230:Limit of Detection; D010209:Paper; D013997:Time Factors
chemical_list: D001789:Blood Group Antigens