title: HDAC6 mediates the acetylation of TRIM50.
abstract: The E3 Ubiquitin ligase TRIM50 promotes the formation and clearance of aggresome-associated polyubiquitinated proteins through HDAC6 interaction, a tubulin specific deacetylase that regulates microtubule-dependent aggresome formation. In this report we showed that TRIM50 is a target of HDAC6 with Lys-372 as a critical residue for acetylation. We identified p300 and PCAF as two TRIM50 acetyltransferases and we further showed that a balance between ubiquitination and acetylation regulates TRIM50 degradation.
authors.affiliation: Medical Genetics Unit, IRCCS Casa Sollievo Della Sofferenza Hospital, 71013 San Giovanni Rotondo, Italy.
authors.forename: Carmela
authors.initials: C
authors.lastname: Fusco
authors.affiliation: Medical Genetics Unit, IRCCS Casa Sollievo Della Sofferenza Hospital, 71013 San Giovanni Rotondo, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Micale
authors.affiliation: Medical Genetics Unit, IRCCS Casa Sollievo Della Sofferenza Hospital, 71013 San Giovanni Rotondo, Italy.
authors.forename: Bartolomeo
authors.initials: B
authors.lastname: Augello
authors.affiliation: Medical Genetics Unit, IRCCS Casa Sollievo Della Sofferenza Hospital, 71013 San Giovanni Rotondo, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Mandriani
authors.affiliation: Medical Genetics Unit, IRCCS Casa Sollievo Della Sofferenza Hospital, 71013 San Giovanni Rotondo, Italy.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Pellico
authors.affiliation: Medical Genetics Unit, IRCCS Casa Sollievo Della Sofferenza Hospital, 71013 San Giovanni Rotondo, Italy.
authors.forename: Pasquelena
authors.initials: P
authors.lastname: De Nittis
authors.affiliation: Medical Genetics Unit, IRCCS Casa Sollievo Della Sofferenza Hospital, 71013 San Giovanni Rotondo, Italy; Telethon Institute of Genetics and Medicine, Via P. Castellino 111, 80131 Naples, Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Calcagnì
authors.affiliation: CEINGE Advanced Biotechnology and Department of Organic Chemistry and Biochemistry, Federico II University, 80131 Napoli, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Monti
authors.affiliation: CEINGE Advanced Biotechnology and Department of Organic Chemistry and Biochemistry, Federico II University, 80131 Napoli, Italy.
authors.forename: Flora
authors.initials: F
authors.lastname: Cozzolino
authors.affiliation: CEINGE Advanced Biotechnology and Department of Organic Chemistry and Biochemistry, Federico II University, 80131 Napoli, Italy.
authors.forename: Piero
authors.initials: P
authors.lastname: Pucci
authors.affiliation: Medical Genetics Unit, IRCCS Casa Sollievo Della Sofferenza Hospital, 71013 San Giovanni Rotondo, Italy; Scienze della Riproduzione e dello Sviluppo, University of Trieste, Italy. Electronic address: g.merla@operapadrepio.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Merla
mesh_terms: D000107:Acetylation; D000818:Animals; D002460:Cell Line; D057809:HEK293 Cells; D006367:HeLa Cells; D000073864:Histone Deacetylase 6; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008870:Microtubules / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases / Q000737:chemistry / Q000378:metabolism*; D054875:Ubiquitination; D050880:p300-CBP Transcription Factors / Q000378:metabolism; D000099224:p300-CBP-Associated Factor
chemical_list: D000073864:Histone Deacetylase 6; D006655:Histone Deacetylases; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor; C579317:TRIM50 protein, human; C433811:HDAC6 protein, human
keywords: Acetylation; HDAC6; NES; TRIM50; Ubiquitination