title: Use of LC-MS for the quality control of radiopharmaceuticals: example of [(18)F]ML10.
abstract: [(18)F]ML10 is a promising novel low molecular weight positron emission tomography probe for apoptosis. As part of the quality control to support clinical studies for cancer therapy monitoring in the GSK Clinical Imaging Centre, a simple and sensitive liquid chromatography mass spectrometry method has been developed and validated for the quantification of total ML10 and impurity content in the final product. Chromatographic separation of ML10 and its radiolabelling precursor and impurities was achieved. Mass curves were constructed from a concentration range of ML10 and known impurities and were linear. Quantification was achieved by comparison of the area under the curve for ML10 content (m/z = 205) and the mass curve. The method was validated over a concentration range of 0.1-1 µg/ml.
authors.affiliation: GSK-Clinical Imaging Centre, Molecular Imaging, Du Cane Road, Hammersmith Hospital, London, W12 0NN, UK.
authors.forename: Richard R
authors.initials: RR
authors.lastname: Reiley
authors.forename: Mickael
authors.initials: M
authors.lastname: Huiban
authors.forename: Idriss
authors.initials: I
authors.lastname: Bennacef
authors.forename: Jan
authors.initials: J
authors.lastname: Passchier
mesh_terms: D005462:Fluorine Radioisotopes / Q000737:chemistry / Q000592:standards*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008764:Methylmalonic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D011786:Quality Control; D019275:Radiopharmaceuticals / Q000737:chemistry / Q000592:standards*
chemical_list: C532430:5-fluoropentyl-2-methylmalonic acid; D005462:Fluorine Radioisotopes; D019275:Radiopharmaceuticals; D008764:Methylmalonic Acid
keywords: LCMS; PET; apoptosis; quality control