title: Three Variants of or near VEGF-A Gene are Not Associated with Neovascular Age-Related Macular Degeneration and Polypoidal Choroidal Vasculopathy in a Han Chinese Population.
abstract: OBJECTIVE
  To investigate whether three previously identified variants for age-related macular degeneration (AMD), the single nucleotide polymorphism (SNP) variants of or near the vascular endothelial growth factor A gene (VEGFA), were associated with neovascular AMD or polypoidal choroidal vasculopathy (PCV) in a Han Chinese cohort.
  
  
  METHODS
  This was a case-control study comprising 251 PCV patients, 157 neovascular AMD patients, and 204 control participants in a Han Chinese population. The rs833069, rs943080 and rs4711751 SNP were genotyped using the Multiplex SNaPshot system. Genotypes and allele frequencies of patients and controls were evaluated for the SNPs using PLINK software.
  
  
  RESULTS
  None of the allelic or genotypic effects of these three variants was significantly associated with PCV, neovascular AMD or combined both patient categories.
  
  
  CONCLUSIONS
  No association was found to support the role for the rs833069, rs943080 and rs4711751 variants of or near VEGFA gene in susceptibility to either PCV or neovascular AMD in Han Chinese population. Further replication is necessary to validate these results.
authors.affiliation: a State Key Laboratory of Ophthalmology , Zhongshan Ophthalmic Center, Sun Yat-sen University , Guangzhou , China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Su
authors.forename: Xiongze
authors.initials: X
authors.lastname: Zhang
authors.forename: Chengguo
authors.initials: C
authors.lastname: Zuo
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Kunfang
authors.initials: K
authors.lastname: Wu
authors.forename: Yuying
authors.initials: Y
authors.lastname: Ji
authors.forename: Feng
authors.initials: F
authors.lastname: Wen
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D020256:Choroidal Neovascularization / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D060005:Genotyping Techniques; D006801:Humans; D008297:Male; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011127:Polyps / Q000235:genetics*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*; D057135:Wet Macular Degeneration / Q000235:genetics*
chemical_list: D017931:DNA Primers; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: Han Chinese population; neovascular age-related macular degeneration; polymorphisms; polypoidal choroidal vasculopathy; vascular endothelial growth factor A