title: [Microarc oxidation of titanium surfaces on osteoblast morphology and cytoskeleton].
abstract: OBJECTIVE
  This study aimed to evaluate the effects of the microarc oxidation surface on cell morphology and cytoskeleton.
  
  
  METHODS
  Pure titanium with a diameter of 15 mm and a thickness of 1 mm was divided into four groups: grooved surfaces (group G); sandblasted surfaces (group SB); grooved microarc oxidation surfaces (group GMAO); and sandblasted microarc oxidation surfaces (group SBMAO). Osteoblast cells were cultured in each group. The morphology and proliferation of the cells on the titanium surface were observed by scanning electron microscope (SEM). The cytoskeleton was evaluated by laser scanning confocal microscope (LSCM).
  
  
  RESULTS
  The osteoblasts were inoculated after 12 h; these osteoblasts then spread along the surface of the titanium plate. In GMAO and SBMAO groups, the osteoblasts converged in the hole. The actin fibers in each group were clearly visible. In particular, the actin fibers in GMAO and SBMAO groups were arranged in parallel and formed bundles that extended into the holes.
  
  
  CONCLUSIONS
  After microarc oxidation, the titanium surface significantly affected the morphology and cytoskeleton of osteoblasts.
authors.affiliation: Dept. of Prosthodontics, The Affiliated Stomatological Hospital of Kunming Medical University, Kunming 650031, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Qiao
authors.forename: Zhongjuan
authors.initials: Z
authors.lastname: Ding
authors.forename: Liya
authors.initials: L
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Niu
mesh_terms: D000199:Actins; D003599:Cytoskeleton; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008870:Microtubules; D010006:Osteoblasts; D010084:Oxidation-Reduction; D013499:Surface Properties; D014025:Titanium
chemical_list: D000199:Actins; D014025:Titanium