title: Molecular characterization and in silico analysis of naturally occurring TEM beta-lactamase variants among pathogenic Enterobacteriaceae infecting Indian patients.
abstract: Cephalosporin resistance, particularly due to bla(TEM) encoded β-lactamases, among Enterobacteriaceae is, though, an increasing public health problem in India; their circulating genetic variants remain unknown. The present study deals with determination of bla(TEM) variants among 134 pathogenic Enterobacteriaceae of Indian origin. Their resistance profile against 3rd generation cephalosporins was determined. The presence of bla(TEM) variants among the bacterial plasmids was characterized by PCR followed by sequencing. Intergenic relations among the variants was determined by phylogenetic analysis. bla(TEM) protein were modeled by Modeller9v5 and verified. The catalytic pockets were characterized, and their interaction with cephalosporins was analyzed using AutoDock tools. More than 87% of isolates showed cephalosporin resistance with ESBL production among 57.8% of Escherichia coli and 50.6% of klebsiella pneumoniae. bla(TEM-1) (84.21%), bla(TEM-1) like (3.94%), bla(TEM-33) (3.94%), bla(TEM-116) (3.94%), bla(TEM-169) (3.94%), and bla(TEM-190) (7.89%) were detected in 76 isolates. Four variants, namely, bla(TEM-1) like, bla(TEM-33), bla(TEM-169), and bla(TEM-190), coexisted in 3 isolates. The largest catalytic pocket of bla(TEM-33) explained its expanded activity towards β-lactam-β-lactamase inhibitor combinations. Molecular docking indicated differential resistance pattern of bla(TEM) variants.
authors.affiliation: Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, 108 C. R. Avenue, Kolkata 700073, India.
authors.forename: Lena
authors.initials: L
authors.lastname: Dhara
authors.forename: Anusri
authors.initials: A
authors.lastname: Tripathi
authors.forename: Arijit
authors.initials: A
authors.lastname: Pal
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli; D004927:Escherichia coli Infections / Q000201:enzymology / Q000235:genetics; D005260:Female; D006801:Humans; D007194:India; D007710:Klebsiella Infections / Q000201:enzymology / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics; D008297:Male; D062105:Molecular Docking Simulation; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases