title: Detection of Quorum Sensing Signal Molecules in Edwardsiella ictaluri Ei-151.
abstract: Edwardsiella ictaluri is a Gram-negative pathogenic bacterium in the family Enterobacteriaceae that causes enteric septicemia of catfish, which has become a significant problem in the aquaculture of striped catfish (Pangasianodon hypophthalmus) in Vietnam. In this study, a bacterium designated as Ei-151 was isolated from diseased striped catfish and proved to be virulent. Based on 16S rDNA sequencing and phenotypic tests, the pathogenic bacterium was identified as Edw. ictaluri. The presence of quorum sensing signal molecules in Edw. ictaluri Ei-151 was detected with different biosensor strains. The results showed that Ei-151 produced at least three kinds of acylated homoserine lactone (AHL) signal molecules as detected with the biosensor Agrobacterium tumefaciens KYC55, and the AHLs fingerprint was similar to that of Edw. tarda. During its entire growth, the levels of AHLs and autoinducer-2 produced by Ei-151 peaked at the stationary phase (OD600 1.8), which suggested that both of them may function at the stationary phase. No Cholerae autoinducer-1-like activity (including Edw. ictaluri LMG7860(T)) was detected.
authors.affiliation: College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao, 266003 China ; Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
authors.forename: Yin
authors.initials: Y
authors.lastname: Han
authors.forename: Nguyen Thi Ngoc
authors.initials: NT
authors.lastname: Tinh
authors.forename: Nguyen Thi
authors.initials: NT
authors.lastname: Hien
authors.forename: Peter
authors.initials: P
authors.lastname: Bossier
keywords: Autoinducer-2 (AI-2); Cholerae autoinducer-1 (CAI-1); Edwardsiella ictaluri; N-acylhomoserine lactones (AHLs); Quorum sensing