title: In search of an accurate evaluation of intrahepatic cholestasis of pregnancy.
abstract: Until now, biochemical parameter for diagnosis of intrahepatic cholestasis of pregnancy (ICP) mostly used is the rise of total serum bile acids (TSBA) above the upper normal limit of 11 μM. However, differential diagnosis is very difficult since overlapped values calculated on bile acids determinations, are observed in different conditions of pregnancy including the benign condition of pruritus gravidarum. The aim of this work was to determine the better markers in ICP for a precise diagnosis together with parameters associated with severity of symptoms and treatment evaluation. Serum bile acid profiles were evaluated using capillary electrophoresis in 38 healthy pregnant women and 32 ICP patients and it was calculated the sensitivity, specificity, accuracy, predictive values and the relationships of certain individual bile acids in pregnant women in order to replace TSBA determinations. The evaluation of the results shows that LCA and UDCA/LCA ratio provided information for a more complete and accurate diagnosis and evaluation of ICP than calculation of solely TSBA levels in pregnant women.
authors.affiliation: Department of Analytical Chemistry and Physicochemistry, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Junin 956, 1113 Buenos Aires, Argentina.
authors.forename: Manuela
authors.initials: M
authors.lastname: Martinefski
authors.forename: Mario
authors.initials: M
authors.lastname: Contin
authors.forename: Silvia
authors.initials: S
authors.lastname: Lucangioli
authors.forename: Maria Beatriz
authors.initials: MB
authors.lastname: Di Carlo
authors.forename: Valeria
authors.initials: V
authors.lastname: Tripodi