title: Protective effects of skin permeable epidermal and fibroblast growth factor against ultraviolet-induced skin damage and human skin wrinkles.
abstract: BACKGROUND
  Epidermal and fibroblast growth factor (EGF and FGF1) proteins play an important role in the regeneration and proliferation of skin cells. EGF and FGF1 have considerable potential as possible therapeutic or cosmetic agents for the treatment of skin damage including wrinkles.
  
  
  OBJECTIVE
  Using protein transduction domains (PTD), we investigated whether PTD-EGF and FGF1 transduced into skin cells and tissue. Transduced proteins showed protective effects in a UV-induced skin damage model as well as against skin wrinkles.
  
  
  METHODS
  Transduced PTD-EGF and FGF1 proteins were detected by immunofluorescence and immunohistochemistry. The effects of PTD-EGF and FGF1 were examined by WST assay, Western blotting, immunohistochemistry, and skin wrinkle parameters.
  
  
  RESULTS
  The PTD-EGF and FGF1 increased cell proliferation and collagen type 1 alpha 1 protein accumulation in skin tissue. Also, PTD-EGF and FGF1 inhibited UV-induced skin damage. Furthermore, topical application of PTD-EGF and FGF1 contained ampoules which were considered to improve the wrinkle parameters of human skin.
  
  
  CONCLUSIONS
  These results show that PTD-EGF and FGF1 can be a potential therapeutic or cosmetic agent for skin damaged and injury including wrinkles and aging.
authors.affiliation: Bioceltran co., Ltd., Chuncheon, Gangwon-do, South Korea; Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon, Korea.
authors.forename: Jae Jin
authors.initials: JJ
authors.lastname: An
authors.forename: Won Sik
authors.initials: WS
authors.lastname: Eum
authors.forename: Hyuck Se
authors.initials: HS
authors.lastname: Kwon
authors.forename: Jae Sook
authors.initials: JS
authors.lastname: Koh
authors.forename: Soo Yun
authors.initials: SY
authors.lastname: Lee
authors.forename: Ji Hwoon
authors.initials: JH
authors.lastname: Baek
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Cho
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Kim
authors.forename: Kyu Huyng
authors.initials: KH
authors.lastname: Han
authors.forename: Jinseu
authors.initials: J
authors.lastname: Park
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Jang
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
mesh_terms: D000818:Animals; D003879:Dermatologic Agents / Q000494:pharmacology*; D004195:Disease Models, Animal; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D012867:Skin / Q000187:drug effects*; D015595:Skin Aging / Q000528:radiation effects*; D016896:Treatment Outcome; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D003879:Dermatologic Agents; D016220:Fibroblast Growth Factor 1; D004815:Epidermal Growth Factor
keywords: PTD-EGF and FGF1; UV; protein therapy; skin permeation; wrinkle