title: Proteomic analysis of membrane proteins of vero cells: exploration of potential proteins responsible for virus entry.
abstract: Vero cells are highly susceptible to many viruses in humans and animals, and its membrane proteins (MPs) are responsible for virus entry. In our study, the MP proteome of the Vero cells was investigated using a shotgun LC-MS/MS approach. Six hundred twenty-seven proteins, including a total of 1839 peptides, were identified in MP samples of the Vero cells. In 627 proteins, 307 proteins (48.96%) were annotated in terms of biological process of gene ontology (GO) categories; 356 proteins (56.78%) were annotated in terms of molecular function of GO categories; 414 proteins (66.03%) were annotated in terms of cellular components of GO categories. Of 627 identified proteins, seventeen proteins had been revealed to be virus receptor proteins. The resulting protein lists and highlighted proteins may provide valuable information to increase understanding of virus infection of Vero cells.
authors.affiliation: College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University , Daqing, People's Republic of China .
authors.forename: Donghua
authors.initials: D
authors.lastname: Guo
authors.forename: Qinghe
authors.initials: Q
authors.lastname: Zhu
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Dongbo
authors.initials: D
authors.lastname: Sun
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D002853:Chromatography, Liquid; D018390:Gene Targeting; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D040901:Proteomics; D053719:Tandem Mass Spectrometry; D014709:Vero Cells; D053586:Virus Internalization
chemical_list: D008565:Membrane Proteins