title: Multiple host-cell recombination pathways act in Agrobacterium-mediated transformation of plant cells.
abstract: Using floral-dip, tumorigenesis and root callus transformation assays of both germline and somatic cells, we present here results implicating the four major non-homologous and homologous recombination pathways in Agrobacterium-mediated transformation of Arabidopsis thaliana. All four single mutant lines showed similar mild reductions in transformability, but knocking out three of four pathways severely compromised Agrobacterium-mediated transformation. Although integration of T-DNA into the plant genome is severely compromised in the absence of known DNA double-strand break repair pathways, it does still occur, suggesting the existence of other pathways involved in T-DNA integration. Our results highlight the functional redundancy of the four major plant recombination pathways in transformation, and provide an explanation for the lack of strong effects observed in previous studies on the roles of plant recombination functions in transformation.
authors.affiliation: Génétique, Reproduction et Développement, UMR CNRS 6293, Clermont Université, INSERM U1103, UFR Sciences et Technologies, Université Blaise Pascal, 24 avenue des Landais, BP80026, 63171, Aubière Cedex, France.
authors.forename: Imen
authors.initials: I
authors.lastname: Mestiri
authors.forename: Frédéric
authors.initials: F
authors.lastname: Norre
authors.forename: Maria E
authors.initials: ME
authors.lastname: Gallego
authors.forename: Charles I
authors.initials: CI
authors.lastname: White
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics* / Q000382:microbiology; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D035264:Flowers / Q000235:genetics / Q000382:microbiology; D055786:Gene Knockout Techniques; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D018745:Genome, Plant / Q000235:genetics*; D009154:Mutation; D018517:Plant Roots / Q000235:genetics / Q000382:microbiology; D010941:Plant Tumors; D030821:Plants, Genetically Modified; D011995:Recombination, Genetic; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial; D018744:DNA, Plant; C036483:T-DNA
keywords: Agrobacterium; Arabidopsis; T-DNA; integration; recombination; transformation