title: Fluorescent aptasensor based on aggregation-induced emission probe and graphene oxide.
abstract: Recently, a great variety of aggregation-induced emission (AIE)-active molecules has been utilized to design bioprobes for label-free fluorescent turn-on aptasensing with high sensitivity. However, due to nonspecific binding interaction between aptamer and AIE probe, these AIE-based aptasensors have nearly no selectivity, thereby significantly limiting the development. In this work, a 9,10-distyrylanthracene with two ammonium groups (DSAI) is synthesized as a novel AIE probe, and the fluorescent aptasensor based on DSAI and graphene oxide (GO) is developed for selective and sensitive sensing of targeted DNA and thrombin protein. Given its AIE property and high selectivity and sensitivity, this aptasensor can be also exploited to detect other targets.
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Jilin University , 2699 Qianjin Avenue, Changchun, Jilin 130012, P. R. China.
authors.forename: Xing
authors.initials: X
authors.lastname: Li
authors.forename: Ke
authors.initials: K
authors.lastname: Ma
authors.forename: Shoujun
authors.initials: S
authors.lastname: Zhu
authors.forename: Shiyu
authors.initials: S
authors.lastname: Yao
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.forename: Bai
authors.initials: B
authors.lastname: Yang
authors.forename: Wenjing
authors.initials: W
authors.lastname: Tian
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D010087:Oxides; D006108:Graphite