title: Inclusion complex of barbigerone with hydroxypropyl-β-cyclodextrin: preparation and in vitro evaluation.
abstract: The aim of this study was to improve the water solubility of barbigerone by complexing it with hydroxypropyl-β-cyclodextrin (HP-β-CD). The inclusion complexation behavior, characterization and interactions of barbigerone with HP-β-CD were investigated in both solution and the solid state by means of UV/VIS, (1)H NMR, FT-IR, PXRD, SEM. All the characterization information demonstrated the formation of barbigerone-HP-β-CD (bar-HP-β-CD) inclusion complex, and the bar-HP-β-CD inclusion compounds exhibited different spectroscopic features and properties from barbigerone. The results demonstrated that the water solubility of barbigerone was notably increased in the presence of HP-β-CD. Furthermore, preliminary in vitro cytotoxicity assay showed that bar-HP-β-CD still maintain the anticancer activity of barbigerone. These results suggest that HP-β-CD will be potentially useful in the delivery of water-insoluble anticancer agents such as barbigerone.
authors.affiliation: State Key laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, PR China; Department of Bio-pharmaceutical Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, PR China; College of Materials and Chemistry and Chemical Engineering, Chengdu University of Technology, Chengdu 610059, PR China.
authors.forename: Neng
authors.initials: N
authors.lastname: Qiu
authors.forename: Xia
authors.initials: X
authors.lastname: Cheng
authors.forename: Guangcheng
authors.initials: G
authors.lastname: Wang
authors.forename: Wenwen
authors.initials: W
authors.lastname: Wang
authors.forename: Jiaolin
authors.initials: J
authors.lastname: Wen
authors.forename: Yongkui
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hang
authors.initials: H
authors.lastname: Song
authors.forename: Liang
authors.initials: L
authors.lastname: Ma
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Wei
authors.forename: Aihua
authors.initials: A
authors.lastname: Peng
authors.forename: Lijuan
authors.initials: L
authors.lastname: Chen
mesh_terms: D000073738:2-Hydroxypropyl-beta-cyclodextrin; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D007529:Isoflavones / Q000737:chemistry* / Q000494:pharmacology*; D012995:Solubility; D014867:Water / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D007529:Isoflavones; C543999:barbigerone; D047392:beta-Cyclodextrins; D014867:Water; D000073738:2-Hydroxypropyl-beta-cyclodextrin
keywords: Anti-cancer; Barbigerone; Characterization; Hydroxypropyl-β-cyclodextrin; Inclusion complex; Solubility