title: Time-dependent progression from the acute to chronic phases in atopic dermatitis induced by epicutaneous allergen stimulation in NC/Nga mice.
abstract: Atopic dermatitis (AD) is a complicated skin condition influenced by genetic background and environmental factors. In this study, we applied Dermatophagoides farinae body extract (DfE) to the barrier-disrupted skin of NC/Nga mice twice a week for 8 weeks to identify the clinical and immunological factors in AD progression. Repeated application of the DfE to the skin of NC/Nga mice showed the similar consequences for the natural course of progression in human AD, histologically and immunologically. We confirmed that the AD-like skin lesions in NC/Nga mice did not last for the whole period of our experiment in spite of repeated topical applications of DfE twice a week. Topical DfE stimulation increased the skin mRNA expressions of Th1-, Th2- and Th17-related cytokines in the acute phase. The expression patterns of IL-4 and IL-13 in splenic T cells and skin lesions were consistent with the time course alterations of clinical features of AD-like skin symptoms. We also showed that there was a remission phase either just before or right after the chronic phase in this experimental model. Interestingly, splenic T-cell-derived IL-5 expression began to increase in the chronic phase, while skin-derived IL-5 mRNA expression increased in the acute phase. In conclusion, our results suggest that we should pay attention to the characteristics of each stage of AD progression and choose a suitable corresponding stage of animal model not only to elucidate the pathogenesis of AD but also to develop and evaluate therapeutic drugs for AD.
authors.affiliation: Department of Dermatology, Chung-Ang University Hospital, Seoul, South Korea.
authors.forename: Ji-Yun
authors.initials: JY
authors.lastname: Kim
authors.forename: Mi Sook
authors.initials: MS
authors.lastname: Jeong
authors.forename: Mi Kyung
authors.initials: MK
authors.lastname: Park
authors.forename: Mi-Kyung
authors.initials: MK
authors.lastname: Lee
authors.forename: Seong Jun
authors.initials: SJ
authors.lastname: Seo
mesh_terms: D000208:Acute Disease; D000279:Administration, Cutaneous; D000485:Allergens / Q000008:administration & dosage*; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000008:administration & dosage; D002908:Chronic Disease; D016207:Cytokines / Q000096:biosynthesis; D003876:Dermatitis, Atopic / Q000209:etiology* / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000097:blood / Q000145:classification; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D016207:Cytokines; D007136:Immunoglobulins
keywords: Dermatophagoides farinae; NC/Nga mouse; atopic dermatitis; cytokines