title: Male reproductive biology of Aedes mosquitoes.
abstract: Among Aedes mosquitoes are species responsible for transmission of serious pathogens to humans. To cope with the current threats to long-term effectiveness of the traditional vector control methods, non-conventional control strategies are being developed. These include autocidal control such as the release of sterile males (sterile insect technique) and the release of Wolbachia-infected males to induce sexual sterility (incompatible insect technique) and pathogen-refractory strain replacement variations using Wolbachia. Sterile male types of techniques particularly depend on released males' ability to successfully mate with wild females. For that reason, a good understanding of male mating biology, including a thorough understanding of the reproductive system and mating capacity, increases the likelihood of success of such genetic vector control programmes. Here we review the literature concerning the reproduction of Aedes mosquitoes with an emphasis on the male biology. We consider sexual maturation, mate finding, insemination, male reproductive capacity, and the occurrence of multiple matings. We also discuss which parameters are of greatest importance for the successful implementation of autocidal control methods and propose questions for future research.
authors.affiliation: Polo d'Innovazione Genomica, Genetica e Biologia S.C.a.R.L., Edificio C, 3̂ piano Polo Unico di Medicina 'Santa Maria della Misericordia', Loc. S. Andrea delle Fratte, 06132 Perugia, Italy. Electronic address: cleliaoliva@gmail.com.
authors.forename: Clelia F
authors.initials: CF
authors.lastname: Oliva
authors.affiliation: Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, FAO/IAEA Agriculture and Biotechnology Laboratories, A-2444 Seibersdorf, Austria. Electronic address: D.Damiens@iaea.org.
authors.forename: David
authors.initials: D
authors.lastname: Damiens
authors.affiliation: Università di Perugia, Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Perugia, Italy. Electronic address: mqbenedict@yahoo.com.
authors.forename: Mark Q
authors.initials: MQ
authors.lastname: Benedict
mesh_terms: D000330:Aedes / Q000502:physiology*; D000818:Animals; D007246:Infertility; D008297:Male; D009032:Mosquito Control / Q000379:methods; D010572:Pest Control, Biological / Q000379:methods; D012726:Sexual Behavior, Animal
keywords: Aedes; Genetic control; Male; Mate-selection; Reproduction; Sterile insect technique