title: Proteome characterization of splenocytes from an Aβpp/ps-1 Alzheimer's disease model.
abstract: Alzheimer's disease (AD) is the sixth leading cause of US deaths. In addition to neurodegenerative deficits in AD, changes in the immune system have also been observed. Proteomic analysis of specific immune cell populations may help gain insights into mechanisms of peripheral immunity in AD. Herein, we report proteome characterization for two subsets of splenocytes (i.e. CD90+ cells and a heterogeneous pool of CD90- cells) from a double transgenic mutant amyloid precursor protein/presenilin-1 (Aβpp/ps-1) AD mouse model. Overall, 906 proteins were identified from both cell types with 275 and 334 proteins uniquely identified as CD90+ and CD90- cells, respectively. Proteins identified in CD90+ and CD90- cells were significantly involved in 18 and 19 KEGG pathways, respectively. Amongst these, pathways associated with AD and antigen processing and presentation were identified in CD90+ and CD90- subsets, respectively. This is the first study to provide a reference proteome map for splenocyte populations in Aβpp/ps-1 double transgenic mice which will be helpful for future studies focused on understanding peripheral changes in this model. All MS data have been deposited in the ProteomeXchange with identifier PXD000203 (http://proteomecentral.proteomexchange.org/dataset/PXD000203).
authors.affiliation: Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, USA.
authors.forename: Zhiyun
authors.initials: Z
authors.lastname: Cao
authors.forename: Renã A S
authors.initials: RA
authors.lastname: Robinson
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics* / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000235:genetics*; D000818:Animals; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D053764:Presenilin-1 / Q000235:genetics*; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D015398:Signal Transduction; D013154:Spleen / Q000166:cytology*
chemical_list: D016564:Amyloid beta-Protein Precursor; D053764:Presenilin-1; D020543:Proteome
keywords: Alzheimer's disease; Animal proteomics; Aβpp/ps-1 mice; Splenocytes; T cells