title: Autologous and allogenic systems of HIV expansion: what is the better choice for clinical application in therapeutic vaccine?
abstract: OBJECTIVE
  HIV-1 expanded in an allogenic system (Al-HIV) represents a cheaper and faster alternative to the autologous virus (Au-HIV) as an antigen in anti-HIV immunotherapy. In this study, chemically inactivated HIV-1 obtained through autologous or allogenic systems were compared.
  
  
  METHODS
  Au-HIV and Al-HIV obtained from cultures of peripheral blood mononuclear cells from 11 HIV(+) individuals were tested for virus production, yield and time of culture, and their ability to elicit a specific immune response in vitro.
  
  
  RESULTS
  The allogenic system was more efficient than the autologous system. Dendritic cells pulsed with Au-HIV and Al-HIV presented a similar phenotypic profile, but only Al-HIV induced a significant increase in IFN-γ(+) lymphocytes.
  
  
  CONCLUSIONS
  The use of an allogenic system displays several advantages in terms of cell manipulation, time and cost of culture, and immunogenicity.
authors.affiliation: Laboratory of Medical Investigation in Dermatology & Immunodeficiencies LIM-56, Department of Dermatology, Faculty of Medicine, University of São Paulo, Av Dr. Eneas de Carvalho Aguiar, 500, São Paulo, Brazil.
authors.forename: Lais Teodoro da
authors.initials: LT
authors.lastname: Silva
authors.forename: Alessandra
authors.initials: A
authors.lastname: Pontillo
authors.forename: Wanessa Cardoso
authors.initials: WC
authors.lastname: da Silva
authors.forename: Alexandre de
authors.initials: Ad
authors.lastname: Almeida
authors.forename: Alberto José da Silva
authors.initials: AJ
authors.lastname: Duarte
authors.forename: Telma Miyuki
authors.initials: TM
authors.lastname: Oshiro
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology / Q000627:therapeutic use; D001782:Blood Donors; D017252:CD3 Complex / Q000276:immunology / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology; D005434:Flow Cytometry; D015658:HIV Infections / Q000097:blood* / Q000517:prevention & control / Q000821:virology; D015497:HIV-1 / Q000254:growth & development / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000821:virology; D008213:Lymphocyte Activation / Q000276:immunology; D014779:Virus Replication / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D017252:CD3 Complex; D007371:Interferon-gamma