title: Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination.
abstract: We demonstrate super-resolution imaging with background fluorescence rejection by interferometric temporal focusing microscopy, in which temporal focusing is combined with structured illumination. The lateral resolution and the optical sectioning capability are simultaneously improved by factors of 1.6 and 1.4, respectively, compared to conventional temporal focusing microscopy. Fluorescent beads (200 nm diameter) that are difficult to distinguish from the background fluorescence in conventional temporal focusing microscopy, are clearly visualized by interferometric temporal focusing microscopy.
authors.affiliation: Laser Technology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan ; RIKEN Center for Advanced Photonics, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Isobe
authors.forename: Takanori
authors.initials: T
authors.lastname: Takeda
authors.forename: Kyohei
authors.initials: K
authors.lastname: Mochizuki
authors.forename: Qiyuan
authors.initials: Q
authors.lastname: Song
authors.forename: Akira
authors.initials: A
authors.lastname: Suda
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Kannari
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kawano
authors.forename: Akiko
authors.initials: A
authors.lastname: Kumagai
authors.forename: Atsushi
authors.initials: A
authors.lastname: Miyawaki
authors.forename: Katsumi
authors.initials: K
authors.lastname: Midorikawa
keywords: (180.4315) Nonlinear microscopy; (190.4180) Multiphoton processes