title: Vascular endothelial growth factor promotes peripheral nerve regeneration after sciatic nerve transection in rat.
abstract: OBJECTIVE
  To evaluate the local effect of vascular endothelial growth factor (VEGF) on transected sciatic nerve regeneration.
  
  
  METHODS
  Sixty male white Wistar rats were divided into four experimental groups randomly (n equal to 15). In transected group the left sciatic nerve was transected and the stump was fixed to adjacent muscle. In treatment group the defect was bridged using a silicone graft filled with 10 microlitre VEGF. In silicone group the graft was filled with phosphate-buffered saline. In sham-operated group the sciatic nerve was exposed and manipulated. Each group was subdivided into three subgroups with five animals in each and nerve fibers were studied 4, 8 and 12 weeks after operation.
  
  
  RESULTS
  Behavioral test, functional study of sciatic nerve, gastrocnemius muscle mass and morphometric indices confirmed a faster recovery of regenerated axons in VEGF group than in silicone group (P less than 0.05). In immunohistochemical assessment, reactions to S-100 in VEGF group were more positive than that in silicone group.
  
  
  CONCLUSIONS
  Local administration of VEGF will improve functional recovery and morphometric indices of sciatic nerve.
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia 571531177, Iran.
authors.forename: Rahim
authors.initials: R
authors.lastname: Mohammadi
authors.forename: Sima
authors.initials: S
authors.lastname: Ahsan
authors.forename: Masoume
authors.initials: M
authors.lastname: Masoumi
authors.forename: Keyvan
authors.initials: K
authors.lastname: Amini
mesh_terms: D000287:Administration, Topical; D000818:Animals; D009416:Nerve Regeneration / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000601:surgery; D042461:Vascular Endothelial Growth Factor A
chemical_list: D042461:Vascular Endothelial Growth Factor A