title: Epitope imprinted polymer coating CdTe quantum dots for specific recognition and direct fluorescent quantification of the target protein bovine serum albumin.
abstract: A novel epitope molecularly imprinted polymer (EMIP) for specific recognition and direct fluorescent quantification of the target protein bovine serum albumin (BSA) was demonstrated where polymerization was performed on the surface of silica nanospheres embedded CdTe quantum dots (QDs). The synthetic peptide derived from the surface-exposed C-terminus of bovine serum albumin (BSA, residues 599-607) was selected as the template molecule. The resulting EMIP film was able to selectively capture the template peptide and the corresponding target protein BSA via the recognition cavities. Based on the fluorescence quenching, the EMIP-coated QDs (molecular imprinted polymer coating CdTe QDs using epitope as the template) nanospheres were successfully applied to the direct fluorescence quantification of BSA. Compared with BMIP-coated QDs (molecular imprinted polymer coating CdTe QDs using BSA as the template), the imprinting factor and adsorption capacity of EMIP-coated QDs were greatly increased. The prepared EMIP-coated QDs can also discriminate even one mismatched sequences from the original sequences of the epitope of the BSA. The practical analytical performance of the EMIP-coated QDs was examined by evaluating the detection of BSA in the bovine calf serum sample with satisfactory results. In addition, the resulting EMIP-coated QDs nanospheres were also successfully applied to separating BSA from the bovine blood sample.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, and Department of Chemistry, Nankai University, Tianjin 300071, China; Synergetic Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, China.
authors.forename: Ya-Qiong
authors.initials: YQ
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, and Department of Chemistry, Nankai University, Tianjin 300071, China; Synergetic Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, China.
authors.forename: Xi-Wen
authors.initials: XW
authors.lastname: He
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, and Department of Chemistry, Nankai University, Tianjin 300071, China; Synergetic Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, China.
authors.forename: Yi-Zhi
authors.initials: YZ
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, and Department of Chemistry, Nankai University, Tianjin 300071, China; Synergetic Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, China. Electronic address: wyli@nankai.edu.cn.
authors.forename: Wen-You
authors.initials: WY
authors.lastname: Li
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, and Department of Chemistry, Nankai University, Tianjin 300071, China; Synergetic Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, China; National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China.
authors.forename: Yu-Kui
authors.initials: YK
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D019187:Cadmium Compounds / Q000737:chemistry*; D002417:Cattle; D057230:Limit of Detection; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure; D012710:Serum Albumin, Bovine / Q000032:analysis*; D012822:Silicon Dioxide / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D013499:Surface Properties; D013691:Tellurium / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D011108:Polymers; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D013691:Tellurium; C028337:cadmium telluride
keywords: Bovine serum albumin; Epitope imprinting; Fluorescent quantification; Quantum dots