title: Inhibitory effect of cordycepin on experimental hepatic metastasis of B16-F0 mouse melanoma cells.
abstract: In a previous study performed by our group, we demonstrated that the water extract of Cordyceps sinensis (WECS) significantly prevented tumor metastasis from the spleen to the liver, using B16-F0 mouse melanoma cells as a model. In this study, we investigated the anti-metastatic activity of cordycepin (3'-deoxyadenosine), one of the components of WECS, using an identical model of mice injected with B16-F0 cells into the spleen. All mice inoculated with B16-F0 cells died due to liver metastases via the portal vein from the spleen. Control mice not administered cordycepin exhibited higher serum levels of alanine aminotransferase (ALT) due to damage to the liver by metastasized B16-F0 cells from the spleen, and survival times ranged from 17 to 22 days after tumor inoculation. Cordycepin was intraperitoneally administered to mice, and resulted in significantly lower serum ALT levels and longer survival times than those observed in control mice. Taken together, these results indicate that cordycepin may be the active ingredient in C. sinensis exerting an anti-metastatic effect, and may be a potential candidate anti-metastatic agent.
authors.affiliation: Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, and Institute for Biosciences, Mukogawa Women's University, 11-68, Koshien Kyuban-cho, Nishinomiya, Hyogo 663-8179, Japan. lfp51193@mukogawa-u.ac.jp.
authors.forename: Ayuko
authors.initials: A
authors.lastname: Sato
authors.forename: Noriko
authors.initials: N
authors.lastname: Yoshikawa
authors.forename: Erika
authors.initials: E
authors.lastname: Kubo
authors.forename: Mami
authors.initials: M
authors.lastname: Kakuda
authors.forename: Arisa
authors.initials: A
authors.lastname: Nishiuchi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kimoto
authors.forename: Yuta
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Satomi
authors.initials: S
authors.lastname: Kagota
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Shinozuka
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nakamura
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D003839:Deoxyadenosines / Q000008:administration & dosage*; D004354:Drug Screening Assays, Antitumor; D007274:Injections, Intraperitoneal; D008114:Liver Neoplasms, Experimental / Q000097:blood / Q000517:prevention & control / Q000556:secondary*; D008297:Male; D008546:Melanoma, Experimental / Q000097:blood / Q000188:drug therapy / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation
chemical_list: D000970:Antineoplastic Agents; D003839:Deoxyadenosines; D000410:Alanine Transaminase; C058120:cordycepin
keywords: B16-F0 mouse melanoma cell; Cordycepin; alanine aminotransferase (ALT); hepatic metastasis; survival