title: Evaluation of diagnostic potential of Rv3803c and Rv2626c recombinant antigens in TB endemic country Pakistan.
abstract: To overcome and eliminate tuberculosis (TB), definitive, reliable, and rapid diagnosis is mandatory. Presently, the diagnostic potential of acute and latent stage TB specific antigens i.e., Rv3803c and Rv2626c was determined. Immunogenic recombinant genes of Rv3803c and Rv2626c antigens were cloned in bacterial expression vector pET23b and expressed product was purified. The homogeneity and structural integrity was confirmed by Western blot analysis. Diagnostic potential of Rv3803c and Rv2626c antigens was analyzed using the sera of 140 active TB patients (AFB smear positive) by indirect ELISA. Ten patients of leprosy and 94 healthy individuals were taken as disease and normal control respectively. The data was analyzed using R statistical package. The sensitivity and specificity of Rv3803c in active TB patients was of 69.3% and 76.4% respectively with an area under ROC curve of 0.77, whereas sensitivity and specificity of Rv2626c 77.1% and 85.1%, respectively. The area under ROC curve of Rv2626c was 0.89 which was significantly higher than Rv3803c (p < 0.0001). Recombinant antigens Rv3803c and Rv2626c have potential to be used as diagnostic markers for TB and need to evaluate with other antigens for differential diagnosis of TB.
authors.affiliation: a Pakistan Medical Research Council , Faisalabad , Pakistan.
authors.forename: Sajjad
authors.initials: S
authors.lastname: Ashraf
authors.forename: Muhammad Arif Nadeem
authors.initials: MA
authors.lastname: Saqib
authors.forename: Mirza Zahid
authors.initials: MZ
authors.lastname: Sharif
authors.forename: Aamer Ali
authors.initials: AA
authors.lastname: Khatak
authors.forename: Sadiq Noor
authors.initials: SN
authors.lastname: Khan
authors.forename: Salman Akbar
authors.initials: SA
authors.lastname: Malik
authors.forename: Sabira
authors.initials: S
authors.lastname: Tahseen
authors.forename: Azra
authors.initials: A
authors.lastname: Khanum
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000942:Antigens, Bacterial / Q000097:blood / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005700:Galactosyltransferases / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology; D010154:Pakistan; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D011994:Recombinant Proteins; D005700:Galactosyltransferases; C430543:Rv3808c protein, Mycobacterium tuberculosis