title: Isolation and molecular analysis of porcine encephalomyocarditis virus strain BD2 from northern China.
abstract: Encephalomyocarditis virus (EMCV) can cause acute myocarditis in young pigs or reproductive failure in sows. In this study, an EMCV strain (BD2) was isolated from the suspected piglets with EMCV in China. It was identified by an indirect immunofluorescence assay and reverse-transcription polymerase chain reaction. The virus could reproduce on BHK-21 cells and reach a peak titer by 16 hpi with maximum titers of 10(8.22)TCID50/0.1 ml. Phylogenetic analyses of open reading frame and the VP3/VP1 genes using neighbor-joining method revealed that EMCV isolates fell into two clusters: groups I and II. The BD2 isolate belonged to group I, along with strains NJ08, HB1, BJC3, CBNU, K3, K11, BEL-2887A, GX0601, GXLC, pEC9, and PV21, whereas four strains (D variant, EMCV-B, EMCV-D, and PV2) belonged to group II.
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biological Products, Baoding, Hebei 071001, China; North China Research Center of Animal Epidemic Pathogen Biology, China Agriculture Ministry, Baoding, Hebei 071001, China.
authors.forename: Wanzhe
authors.initials: W
authors.lastname: Yuan
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biological Products, Baoding, Hebei 071001, China; North China Research Center of Animal Epidemic Pathogen Biology, China Agriculture Ministry, Baoding, Hebei 071001, China.
authors.forename: Qinye
authors.initials: Q
authors.lastname: Song
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biological Products, Baoding, Hebei 071001, China; North China Research Center of Animal Epidemic Pathogen Biology, China Agriculture Ministry, Baoding, Hebei 071001, China.
authors.forename: Xiuyuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biological Products, Baoding, Hebei 071001, China; North China Research Center of Animal Epidemic Pathogen Biology, China Agriculture Ministry, Baoding, Hebei 071001, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biological Products, Baoding, Hebei 071001, China; North China Research Center of Animal Epidemic Pathogen Biology, China Agriculture Ministry, Baoding, Hebei 071001, China. Electronic address: vaccine2000@126.com.
authors.forename: Jiguo
authors.initials: J
authors.lastname: Sun
mesh_terms: D000818:Animals; D018188:Cardiovirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D002460:Cell Line; D002681:China / Q000453:epidemiology; D006224:Cricetinae; D004686:Encephalomyocarditis virus / Q000235:genetics / Q000302:isolation & purification*; D019143:Evolution, Molecular; D016679:Genome, Viral; D016366:Open Reading Frames; D010802:Phylogeny; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*
keywords: Encephalomyocarditis virus (EMCV); Isolation; Phylogenetic analysis