title: The expression of miR-25 is increased in colorectal cancer and is associated with patient prognosis.
abstract: MicroRNA-25 (miR-25) has recently been found to be involved in many critical processes in human malignancies. We aimed to investigate the expression pattern and prognostic role of miR-25 in colorectal cancer. Colorectal cancer and adjacent normal specimens from 186 patients who had not received neoadjuvant chemotherapy were collected. The expression of miR-25 was detected with a quantitative real-time PCR assay, and the association of miR-25 with overall patient survival was analyzed via statistical analysis. The results indicated that the level of miR-25 expression was significantly elevated in colorectal cancer compared with the level observed in the adjacent normal tissue. It was also demonstrated that miR-25 expression is associated with tumor invasion, lymph node metastasis, distant metastasis and the TNM stage of colorectal cancer. In addition, a Kaplan-Meier analysis revealed that an increased level of miR-25 expression is associated with a poor overall survival of patients. A multivariate survival analysis also indicated that miR-25 is an independent prognostic marker after adjusting for known prognostic factors. These results prove that miR-25 expression is increased in colorectal cancer and is associated with tumor progression. This study also provides the first evidence that miR-25 is an independent prognostic factor for patients with colorectal cancer, indicating the potential role of miR-25 as a highly specific and sensitive biomarker.
authors.affiliation: Department of General Surgery, Shaanxi Provincial People's Hospital, Youyi Xi Road 256#, Xi'an, 710068, Shaanxi, China, lixiaojun20040912@163.com.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Li
authors.forename: Chunyan
authors.initials: C
authors.lastname: Yang
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Ruipeng
authors.initials: R
authors.lastname: Zhang
authors.forename: Ruiting
authors.initials: R
authors.lastname: Liu
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000378:metabolism* / Q000401:mortality; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; C543351:MIRN25 microRNA, human; D035683:MicroRNAs