title: Absence of DNA damage after 60-Hz electromagnetic field exposure combined with ionizing radiation, hydrogen peroxide, or c-Myc overexpression.
abstract: The principal objective of this study was to assess the DNA damage in a normal cell line system after exposure to 60 Hz of extremely low frequency magnetic field (ELF-MF) and particularly in combination with various external factors, via comet assays. NIH3T3 mouse fibroblast cells, WI-38 human lung fibroblast cells, L132 human lung epithelial cells, and MCF10A human mammary gland epithelial cells were exposed for 4 or 16 h to a 60-Hz, 1 mT uniform magnetic field in the presence or absence of ionizing radiation (IR, 1 Gy), H(2)O(2) (50 μM), or c-Myc oncogenic activation. The results obtained showed no significant differences between the cells exposed to ELF-MF alone and the unexposed cells. Moreover, no synergistic or additive effects were observed after 4 or 16 h of pre-exposure to 1 mT ELF-MF or simultaneous exposure to ELF-MF combined with IR, H(2)O(2), or c-Myc activation.
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea.
authors.forename: Yeung Bae
authors.initials: YB
authors.lastname: Jin
authors.forename: Seo-Hyun
authors.initials: SH
authors.lastname: Choi
authors.forename: Jae Seon
authors.initials: JS
authors.lastname: Lee
authors.forename: Jae-Kyung
authors.initials: JK
authors.lastname: Kim
authors.forename: Ju-Woon
authors.initials: JW
authors.lastname: Lee
authors.forename: Seung-Cheol
authors.initials: SC
authors.lastname: Hong
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Myung
authors.forename: Yun-Sil
authors.initials: YS
authors.lastname: Lee
mesh_terms: D000818:Animals; D002460:Cell Line; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields / Q000009:adverse effects*; D015870:Gene Expression; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D051379:Mice; D009857:Oncogenes / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D011839:Radiation, Ionizing
chemical_list: D016271:Proto-Oncogene Proteins c-myc; D006861:Hydrogen Peroxide