title: In vitro genotoxicity assessment of MTES, GPTES and TEOS, three precursors intended for use in food contact coatings.
abstract: Organoalkoxysilanes are precursors that are used increasingly in the synthesis of food contact coatings. To comply with the EU regulation, their potential toxicity must be assessed, and very little information is known. The genotoxicity of three common precursors was studied, namely, tetraethylorthosilicate (TEOS), methyltriethoxysilane (MTES) and 3-glycidyloxypropyltriethoxysilane (GPTES). By the Ames test, MTES and TEOS were not mutagenic for bacteria. A significant positive response was observed with GPTES in the TA100 and TA1535 strains. The mutagenic effect was more pronounced in the presence of the exogenous metabolic activation system with an increase of the induction factor (ten-fold higher for the TA1535 strain). In the micronucleus assay performed with a human hepatoma cell line (HepG2 cells), GPTES gave negative results even in the presence of an exogenous activation system. To ascertain the possibility of using this precursor in food contact material, its migration must be monitored according to the coating formulation because migration might result in hazardous human exposure.
authors.affiliation: Laboratoire des Multimatériaux et Interfaces, UMR 5615, University of Lyon, Villeurbanne 69100, France.
authors.forename: Krystelle
authors.initials: K
authors.lastname: Lionti
authors.affiliation: Derttech «Packtox», UMR INSERM U866 Nutox Team, Agrosupdijon, Dijon 21000, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Séverin
authors.affiliation: Derttech «Packtox», UMR INSERM U866 Nutox Team, Agrosupdijon, Dijon 21000, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Dahbi
authors.affiliation: Laboratoire des Multimatériaux et Interfaces, UMR 5615, University of Lyon, Villeurbanne 69100, France.
authors.forename: Bérangère
authors.initials: B
authors.lastname: Toury
authors.affiliation: Derttech «Packtox», UMR INSERM U866 Nutox Team, Agrosupdijon, Dijon 21000, France. Electronic address: marie-christine.chagnon@ubourgogne.fr.
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Chagnon
mesh_terms: D018857:Food Packaging; D056945:Hep G2 Cells; D006801:Humans; D009152:Mutagenicity Tests
keywords: Ames test; GPTES; HepG2 cell line; MTES; Micronucleus assay; TEOS