title: Oxidative modification of blood serum proteins in multiple sclerosis after interferon or mitoxantrone treatment.
abstract: This study was aimed at (i) comparison of the usefulness of serum protein oxidation parameters for assessment of oxidative stress (OS) in multiple sclerosis (MS), and (ii) comparison of OS in MS patients subject to various therapies. Elevated glycophore level was noted in relapsing-remitting (RRMS) patients without treatment and patients treated with interferons β1a and β1b (10.33±3.27, 8.02±2.22 and 8.56±2.45 vs control 5.27±0.73 fluorescence units (FU)/mg protein). Advanced oxidation protein products (295±135 vs 83±65nmol/mg protein), carbonyl groups (3.68±1.44nmol/mg protein vs 2.03±0.23nmol/mg protein), kynurenine (7.71±0.1.67 vs 5.5±0.63 FU/mg protein) and N'-formylkynurenine (7.69±0.7 vs 4.97±0.59 FU/mg protein) levels were increased, while thioredoxin level was decreased in RRMS patients without treatment (5.03±2.18 vs 10.83±2.75ng/ml) with respect to control. The level of OS was higher in untreated RRMS patients and in SPMS patients treated with mitoxantrone than in patients treated with interferon.
authors.affiliation: Department of Biochemistry and Cell Biology, University of Rzeszow, ul. Zelwerowicza 4, 35-601 Rzeszów, Poland. Electronic address: isadowska@poczta.fm.
authors.forename: Izabela
authors.initials: I
authors.lastname: Sadowska-Bartosz
authors.affiliation: Department of Neurology in Zabrze, Medical University of Silesia, ul. 3-go Maja 13-15, 41-800 Zabrze, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Adamczyk-Sowa
authors.affiliation: Department of Molecular Biophysics, University of Łódź, Pomorska 141/143, 90-236 Łódź, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Gajewska
authors.affiliation: Department of Biochemistry and Cell Biology, University of Rzeszow, ul. Zelwerowicza 4, 35-601 Rzeszów, Poland; Department of Molecular Biophysics, University of Łódź, Pomorska 141/143, 90-236 Łódź, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bartosz
mesh_terms: D000328:Adult; D062107:Advanced Oxidation Protein Products / Q000097:blood*; D004185:Disability Evaluation; D005260:Female; D019270:Fructosamine / Q000097:blood; D006021:Glycophorins / Q000378:metabolism; D006801:Humans; D007372:Interferons / Q000627:therapeutic use*; D007737:Kynurenine / Q000031:analogs & derivatives / Q000097:blood; D008297:Male; D008875:Middle Aged; D008942:Mitoxantrone / Q000627:therapeutic use*; D009103:Multiple Sclerosis / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology; D018384:Oxidative Stress / Q000187:drug effects; D050050:Protein Carbonylation / Q000187:drug effects; D013879:Thioredoxins / Q000097:blood
chemical_list: D062107:Advanced Oxidation Protein Products; D006021:Glycophorins; C007772:N'-formylkynurenine; D007737:Kynurenine; D019270:Fructosamine; D013879:Thioredoxins; D007372:Interferons; D008942:Mitoxantrone
keywords: AGEs; Carbonyl groups; Mitoxantrone; Multiple sclerosis; Oxidative protein modifications; Thioredoxin