title: Intrahaemocoelic infection of Trichoplusia ni with the baculovirus Autographa californica M nucleopolyhedrovirus does not induce tracheal cell basal lamina remodelling.
abstract: Infection of the lepidopteran insect Trichoplusia ni with the baculovirus Autographa californica M nucleopolyhedrovirus (AcMNPV) by the oral route stimulates activation of host matrix metalloproteases (MMP) and effector caspases, a process dependent on expression of the viral fibroblast growth factor (vFGF). This pathway leads to tracheal cell basal lamina remodelling, enabling virus escape from the primary site of infection, the midgut epithelium, and establishment of efficient systemic infection. In this study, we asked whether the MMP-caspase pathway was also activated following infection by intrahaemocoelic injection. We found that intrahaemocoelic infection did not lead to any observable tracheal cell or midgut epithelium basal lamina remodelling. MMP and caspase activities were not significantly stimulated. We conclude that the main role of the AcMNPV vFGF is in facilitating virus midgut escape.
authors.affiliation: Division of Biology, Kansas State University, Manhattan, KS 66506, USA.
authors.forename: John C
authors.initials: JC
authors.lastname: Means
authors.affiliation: Division of Biology, Kansas State University, Manhattan, KS 66506, USA.
authors.forename: A Lorena
authors.initials: AL
authors.lastname: Passarelli
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000201:enzymology; D020169:Caspases / Q000235:genetics / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000378:metabolism; D054884:Host-Pathogen Interactions; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism; D009036:Moths / Q000201:enzymology / Q000235:genetics / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000502:physiology*; D014132:Trachea / Q000201:enzymology / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D019476:Insect Proteins; D014764:Viral Proteins; D005346:Fibroblast Growth Factors; D020169:Caspases; D020782:Matrix Metalloproteinases