title: Dendrimer-like polymeric DNAs as chemiluminescence probes for amplified detection of telomere DNA on a solid-phase membrane.
abstract: For the first time, an amplified chemiluminescence (CL) detection of the telomere DNA spotted on a nylon membrane is described here, based on the direct hybridization with the CL probe of dendrimer-like polymeric DNAs possessing a large number of guanine moieties. This probe was synthesized by sense and antisense hybridization between Y-shaped DNAs and then could hybridize with the target DNA.
authors.affiliation: Faculty of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8521, Japan. ms-kai@nagasaki-u.ac.jp.
authors.forename: Ahmed F M
authors.initials: AF
authors.lastname: El-Mahdy
authors.forename: Takayuki
authors.initials: T
authors.lastname: Shibata
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kabashima
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kai
mesh_terms: D004247:DNA / Q000032:analysis* / Q000737:chemistry; D050091:Dendrimers; D049449:Luminescence; D008567:Membranes, Artificial; D009693:Nucleic Acid Hybridization; D009757:Nylons; D011108:Polymers / Q000737:chemistry; D016615:Telomere / Q000235:genetics
chemical_list: D050091:Dendrimers; D008567:Membranes, Artificial; D009757:Nylons; D011108:Polymers; D004247:DNA