title: Effect of semen and seminal amyloid on vaginal transmission of simian immunodeficiency virus.
abstract: BACKGROUND
  Semen and semen-derived amyloid fibrils boost HIV infection in vitro but their impact on sexual virus transmission in vivo is unknown. Here, we examined the effect of seminal plasma (SP) and semen-derived enhancer of virus infection (SEVI) on vaginal virus transmission in the SIV/rhesus macaque (Macacca mulatta) model.
  
  
  RESULTS
  A total of 18 non-synchronized female rhesus macaques (six per group) were exposed intra-vaginally to increasing doses of the pathogenic SIVmac239 molecular clone in the presence or absence of SEVI and SP. Establishment of productive virus infection was assessed by measuring plasma viral RNA loads at weekly intervals. We found that the first infections occurred at lower viral doses in the presence of SP and SEVI compared to the control group. Furthermore, the average peak viral loads during acute infection were about 6-fold higher after exposure to SP- and SEVI-treated virus. Overall infection rates after a total of 27 intra-vaginal exposures to increasing doses of SIV, however, were similar in the absence (4 of 6 animals) and presence of SP (5 of 6), or SEVI (4 of 6). Furthermore, the infectious viral doses required for infection varied considerably and did not differ significantly between these three groups.
  
  
  CONCLUSIONS
  Semen and SEVI did not have drastic effects on vaginal SIV transmission in the present experimental setting but may facilitate spreading of virus infection after exposure to low viral doses that most closely approximate the in vivo situation.
authors.forename: Jan
authors.initials: J
authors.lastname: Münch
authors.forename: Ulrike
authors.initials: U
authors.lastname: Sauermann
authors.forename: Maral
authors.initials: M
authors.lastname: Yolamanova
authors.forename: Katharina
authors.initials: K
authors.lastname: Raue
authors.forename: Christiane
authors.initials: C
authors.lastname: Stahl-Hennig
authors.affiliation: Institute of Molecular Virology, Ulm University Medical Center, Meyerhofstrasse 1, 89081 Ulm, Germany. frank.kirchhoff@uni-ulm.de.
authors.forename: Frank
authors.initials: F
authors.lastname: Kirchhoff
mesh_terms: D000682:Amyloid / Q000378:metabolism*; D000818:Animals; D018562:Disease Transmission, Infectious; D005260:Female; D008253:Macaca mulatta; D012661:Semen / Q000821:virology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000635:transmission* / Q000821:virology*; D015302:Simian Immunodeficiency Virus / Q000302:isolation & purification*; D014621:Vagina / Q000821:virology*
chemical_list: D000682:Amyloid