title: Transplantation of autologous minced bladder mucosa for a one-step reconstruction of a tissue engineered bladder conduit.
abstract: Surgical intervention is sometimes needed to create a conduit from the abdominal wall to the bladder for self-catheterization. We developed a method for tissue engineering a conduit for bladder emptying without in vitro cell culturing as a one-step procedure. In a porcine animal model bladder, wall tissue was excised and the mucosa was minced to small particles. The particles were attached to a tube in a 1 : 3 expansion rate with fibrin glue and transplanted back by attaching the tube to the bladder and through the abdominal wall. Sham served as controls. After 4-5 weeks, conduits were assessed in respect to macroscopic and microscopic appearance in 6 pigs. Two pigs underwent radiology before termination. Gross examination revealed a patent conduit with an opening to the bladder. Histology and immunostaining showed a multilayered transitional uroepithelium in all cases. Up to 89% of the luminal surface area was neoepithelialized but with a loose attachment to the submucosa. No epithelium was found in control animals. CT imaging revealed a patent channel that could be used for filling and emptying the bladder. Animals that experienced surgical complications did not form conduits. Minced autologous bladder mucosa can be transplanted around a tubular mold to create a conduit to the urinary bladder without in vitro culturing.
authors.affiliation: Department of Women's and Children's Health and Center of Molecular Medicine, Karolinska Institutet, Q3:03 Astrid Lindgren Children's Hospital, 171 76 Stockholm, Sweden ; Pediatric Surgery, Unit of Urology, Astrid Lindgren Children's Hospital, Karolinska University Hospital, 171 76 Stockholm, Sweden.
authors.forename: Gisela
authors.initials: G
authors.lastname: Reinfeldt Engberg
authors.forename: Johan
authors.initials: J
authors.lastname: Lundberg
authors.forename: Clara Ibel
authors.initials: CI
authors.lastname: Chamorro
authors.forename: Agneta
authors.initials: A
authors.lastname: Nordenskjöld
authors.forename: Magdalena
authors.initials: M
authors.lastname: Fossum
mesh_terms: D000818:Animals; D005260:Female; D007091:Image Processing, Computer-Assisted; D009092:Mucous Membrane / Q000637:transplantation*; D019651:Plastic Surgery Procedures / Q000379:methods*; D013194:Staining and Labeling; D023822:Tissue Engineering / Q000379:methods*; D014182:Transplantation, Autologous; D001743:Urinary Bladder / Q000473:pathology*