title: Determination of aflatoxins B1, B2, G1, and G2 in olive oil, peanut oil, and sesame oil using immunoaffinity column cleanup, postcolumn derivatization, and liquid chromatography with fluorescence detection: first action 2013.05.
abstract: A collaborative study of a method for determination of aflatoxins (AFs) B1, B2, G1, and G2 in olive oil, peanut oil, and sesame oil using immunoaffinity column cleanup, postcolumn derivatization, and LC with fluorescence detection, previously published in J. AOAC Int. 95, 1689-1700 (2012), was approved as First Action 2013.05 on March 29, 2013 by the Method-Centric Committee for Aflatoxins in Edible Oils. The method uses methanol for extraction followed by filtration. The extract is applied to an immunoaffinity column with antibodies specific for AFs, which are then eluted from the column with a methanol solution. Determination and quantification occur using RP-LC with fluorescence detection after postcolumn derivatization. The average recovery of AFs in olive, peanut, and sesame oils in spiked samples (levels between 2.0 and 20.0 microg/kg) ranged from 84 to 92%. The recoveries for AFs B1, B2, G1, and G2 were 86-93, 89-95, 85-97, and 76-85%, respectively. Within-laboratory RSD (RSDr) values for AFs ranged from 3.4 to 10.2%. RSDr values forAF B1, B2, G1, and G2 were 3.5-10.9, 3.2-9.5, 6.5-14.9, and 4.8-14.2%, respectively. Between-laboratory RSD (RSDR) values for AFs were 6.1-14.5%. RSD, values for AFs B1, B2, G1, and G2 were 7.5-15.4, 7.1-14.6, 10.8-18.1, and 7.6-23.7%, respectively. Horwitz ratio values were < or =2 for the analytes in the three matrixes.
authors.affiliation: Shandong Exit-Entry Inspection and Quarantine Bureau of China, Qing Dao, China 266002. leibaoqd@yahoo.com
authors.forename: Lei
authors.initials: L
authors.lastname: Bao
authors.forename: Chengzhu
authors.initials: C
authors.lastname: Liang
authors.forename: Mary W
authors.initials: MW
authors.lastname: Trucksess
authors.forename: Yanli
authors.initials: Y
authors.lastname: Xu
authors.forename: Ning
authors.initials: N
authors.lastname: Lv
authors.forename: Zhenxing
authors.initials: Z
authors.lastname: Wu
authors.forename: Ping
authors.initials: P
authors.lastname: Jing
authors.forename: Fred S
authors.initials: FS
authors.lastname: Fry
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis; D000348:Aflatoxins / Q000032:analysis*; D002846:Chromatography, Affinity / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D005453:Fluorescence; D000069463:Olive Oil; D000074241:Peanut Oil; D010938:Plant Oils / Q000032:analysis*; D012715:Sesame Oil / Q000032:analysis*
chemical_list: D000348:Aflatoxins; D000069463:Olive Oil; D000074241:Peanut Oil; D010938:Plant Oils; C027955:aflatoxin G1; C029754:aflatoxin G2; C029753:aflatoxin B2; D012715:Sesame Oil; D016604:Aflatoxin B1