title: Notch-Nrf2 axis: regulation of Nrf2 gene expression and cytoprotection by notch signaling.
abstract: The Notch signaling pathway enables regulation and control of development, differentiation, and homeostasis through cell-cell communication. Our investigation shows that Notch signaling directly activates the Nrf2 stress adaptive response pathway through recruitment of the Notch intracellular domain (NICD) transcriptosome to a conserved Rbpjκ site in the promoter of Nrf2. Stimulation of Notch signaling through Notch ligand expression in cells and by overexpression of the NICD in Rosa(NICD/-)::AlbCre mice in vivo induces expression of Nrf2 and its target genes. Continuous and transient NICD expression in the liver produces a Notch-dependent cytoprotective response through direct transcriptional activation of Nrf2 signaling to rescue mice from acute acetaminophen toxicity. This response can be reversed upon genetic disruption of Nrf2. Morphological studies showed that the characteristic phenotype of high-density intrahepatic bile ducts and enlarged liver in Rosa(NICD/-)::AlbCre mice could be at least partially reversed after Nrf2 disruption. Furthermore, the liver and bile duct phenotypes could be recapitulated with constitutive activation of Nrf2 signaling in Keap1(F/F)::AlbCre mice. It appears that Notch-to-Nrf2 signaling is another important determinant in liver development and function and promotes cell-cell cytoprotective signaling responses.
authors.affiliation: Department of Pharmacology and Chemical Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: Nobunao
authors.initials: N
authors.lastname: Wakabayashi
authors.forename: John J
authors.initials: JJ
authors.lastname: Skoko
authors.forename: Dionysios V
authors.initials: DV
authors.lastname: Chartoumpekis
authors.forename: Shoko
authors.initials: S
authors.lastname: Kimura
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Slocum
authors.forename: Kentaro
authors.initials: K
authors.lastname: Noda
authors.forename: Dushani L
authors.initials: DL
authors.lastname: Palliyaguru
authors.forename: Masahiro
authors.initials: M
authors.lastname: Fujimuro
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Boley
authors.forename: Yugo
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Norihisa
authors.initials: N
authors.lastname: Shigemura
authors.forename: Shyam
authors.initials: S
authors.lastname: Biswal
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Kensler
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D019610:Cytoprotection / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology; D006801:Humans; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein / Q000235:genetics* / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D051267:NF-E2-Related Factor 2 / Q000235:genetics*; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics* / Q000502:physiology; D015533:Transcriptional Activation / Q000235:genetics
chemical_list: D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; C495636:Nfe2l2 protein, mouse; D051880:Receptors, Notch