title: A murine model of subarachnoid hemorrhage.
abstract: In this video publication a standardized mouse model of subarachnoid hemorrhage (SAH) is presented. Bleeding is induced by endovascular Circle of Willis perforation (CWp) and proven by intracranial pressure (ICP) monitoring. Thereby a homogenous blood distribution in subarachnoid spaces surrounding the arterial circulation and cerebellar fissures is achieved. Animal physiology is maintained by intubation, mechanical ventilation, and continuous on-line monitoring of various physiological and cardiovascular parameters: body temperature, systemic blood pressure, heart rate, and hemoglobin saturation. Thereby the cerebral perfusion pressure can be tightly monitored resulting in a less variable volume of extravasated blood. This allows a better standardization of endovascular filament perforation in mice and makes the whole model highly reproducible. Thus it is readily available for pharmacological and pathophysiological studies in wild type and genetically altered mice.
authors.affiliation: Institute for Stroke and Dementia Research (ISD), University of Munich Medical Center.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Schüller
authors.forename: Dominik
authors.initials: D
authors.lastname: Bühler
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Plesnila
mesh_terms: D000818:Animals; D002941:Circle of Willis / Q000601:surgery; D004195:Disease Models, Animal; D007427:Intracranial Pressure; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013345:Subarachnoid Hemorrhage