title: A novel tool for dynamic cell adhesion studies--the De-Adhesion Number Investigator DANI.
abstract: For an optimal implementation of materials, such as, e.g. medical implants in living environments, a thorough characterization of cell adhesion, kinetics and strength is required, as well as a prerequisite e.g. for bone integration. Here we present a miniaturized (~100 μl) lab-on-a-chip implant hybrid system which allows quantification of cell adhesion under dynamic conditions mimicking those of physiological relevance. Surface acoustic waves are excited and used on optical transparent chips to induce micro acoustic streaming and to create a microfluidic shear spectrum ranging from 0 to ~35 s(-1). We demonstrate its potential for a time-efficient, dynamic screening test of new implant materials using a model of an osseointegration with SAOS-2 cells. The upside-down orientation also allows utilization of the micro reactor on non-transparent materials like titanium and diamond-like-carbon (DLC).
authors.affiliation: Experimental Physics I, University of Augsburg, 86159 Augsburg, Germany. achim.wixforth@physik.uni-augsburg.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Hartmann
authors.forename: Melanie
authors.initials: M
authors.lastname: Stamp
authors.forename: Ralf
authors.initials: R
authors.lastname: Kmeth
authors.forename: Sascha
authors.initials: S
authors.lastname: Buchegger
authors.forename: Bernd
authors.initials: B
authors.lastname: Stritzker
authors.forename: Belma
authors.initials: B
authors.lastname: Saldamli
authors.forename: Rainer
authors.initials: R
authors.lastname: Burgkart
authors.forename: Matthias F
authors.initials: MF
authors.lastname: Schneider
authors.forename: Achim
authors.initials: A
authors.lastname: Wixforth
mesh_terms: D002244:Carbon / Q000737:chemistry; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D006801:Humans; D006863:Hydrogen-Ion Concentration; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D008904:Miniaturization; D033081:Shear Strength; D013016:Sound; D013696:Temperature; D014025:Titanium / Q000737:chemistry
chemical_list: D002244:Carbon; D014025:Titanium