title: Angiotensin II potentiates zinc-induced cortical neuronal death by acting on angiotensin II type 2 receptor.
abstract: BACKGROUND
  The angiotensin system has several non-vascular functions in the central nervous system. For instance, inhibition of the brain angiotensin system results in a reduction in neuronal death following acute brain injury such as ischemia and intracerebral hemorrhage, even under conditions of constant blood pressure. Since endogenous zinc has been implicated as a key mediator of ischemic neuronal death, we investigated the possibility that the angiotensin system affects the outcome of zinc-triggered neuronal death in cortical cell cultures.
  
  
  RESULTS
  Exposure of cortical cultures containing neurons and astrocytes to 300 μM zinc for 15 min induced submaximal death in both types of cells. Interestingly, addition of angiotensin II significantly enhanced the zinc-triggered neuronal death, while leaving astrocytic cell death relatively unchanged. Both type 1 and 2 angiotensin II receptors (AT1R and AT2R, respectively) were expressed in neurons as well as astrocytes. Zinc neurotoxicity was substantially attenuated by PD123319, a specific inhibitor of AT2R, and augmented by CGP42112, a selective activator of AT2R, indicating a critical role for this receptor subtype in the augmentation of neuronal cell death.Because zinc toxicity occurs largely through oxidative stress, the levels of superoxides in zinc-treated neurons were assessed by DCF fluorescence microscopy. Combined treatment with zinc and angiotensin II substantially increased the levels of superoxides in neurons compared to those induced by zinc alone. This increase in oxidative stress by angiotensin II was completely blocked by the addition of PD123319. Finally, since zinc-induced oxidative stress may be caused by induction and/or activation of NADPH oxidase, the activation status of Rac and the level of the NADPH oxidase subunit p67phox were measured. Angiotensin II markedly increased Rac activity and the levels of p67phox in zinc-treated neurons and astrocytes in a PD123319-dependent manner.
  
  
  CONCLUSIONS
  The present study shows that the angiotensin system, especially that involving AT2R, may have an oxidative injury-potentiating effect via augmentation of the activity of NADPH oxidase. Hence, blockade of angiotensin signaling cascades in the brain may prove useful in protecting against the oxidative neuronal death that is likely to occur in acute brain injury.
authors.affiliation: Neural Injury Research Lab, University of Ulsan College of Medicine, Seoul 138-736, South Korea. jkko@amc.seoul.kr.
authors.forename: Mi-Ha
authors.initials: MH
authors.lastname: Park
authors.forename: Ha Na
authors.initials: HN
authors.lastname: Kim
authors.forename: Joon Seo
authors.initials: JS
authors.lastname: Lim
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Ahn
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Koh
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000473:pathology*; D004789:Enzyme Activation / Q000187:drug effects; D051379:Mice; D019255:NADPH Oxidases / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology*; D018384:Oxidative Stress / Q000187:drug effects; D044139:Receptor, Angiotensin, Type 2 / Q000378:metabolism*; D015032:Zinc / Q000633:toxicity*
chemical_list: D044139:Receptor, Angiotensin, Type 2; D000804:Angiotensin II; D019255:NADPH Oxidases; D015032:Zinc