title: A heteropolysaccharide, L-fuco-D-manno-1,6-α-D-galactan extracted from Grifola frondosa and antiangiogenic activity of its sulfated derivative.
abstract: The tumor growth and metastasis are angiogenesis dependent, thus blockade of angiogenesis is a promising approach for treatment of cancer. Herein we reported the structural and biological features of a novel water-soluble polysaccharide named GFPW from the fruit body of Grifola frondosa. Chemical and spectral analysis revealed that GFPW with an average molecular weight of 15.7 kDa, possessed a backbone consisting of α-1,6-linked galactopyranosyl residues, with branches attached to O-2 of α-1,3-linked fucose residues and α-terminal mannose. By the chlorosulfonic acid-pyridine method, we prepared a sulfated derivative of GFPW, Sul-GFPW, with a substitution degree of 0.33. According to the (13)C NMR spectrum, the substitution position was deduced at C-2 and C-3. The angiogenesis assays in vitro showed that Sul-GFPW significantly inhibited endothelial cell proliferation in a dose- and time-dependent manner, and reduced endothelial cell migration and tube formation as well.
authors.affiliation: Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Shen
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Liao
authors.forename: Jianping
authors.initials: J
authors.lastname: Fang
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
authors.forename: Qun
authors.initials: Q
authors.lastname: Dong
authors.forename: Kan
authors.initials: K
authors.lastname: Ding
mesh_terms: D020533:Angiogenesis Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002240:Carbohydrate Sequence; D002465:Cell Movement / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D005685:Galactans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D048691:Grifola / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D013431:Sulfates / Q000737:chemistry*
chemical_list: D020533:Angiogenesis Inhibitors; D005685:Galactans; D009005:Monosaccharides; D013431:Sulfates; C000590735:sulfated fuco-manno-1,6-galactan
keywords: Antiangiogenesis; Galactan; Grifola frondosa; Human microvascular endothelial cell line-1 (HMEC-1)