title: TNF-α gene expression in subcutaneous adipose tissue associated with HOMA in Asian Indian postmenopausal women.
abstract: The present study determines the fat depot-specific expression of leptin and TNF-α and its association with biochemical parameters in postmenopausal women. A total of 108 postmenopausal women were recruited prospectively; 54 were with metabolic syndrome (cases) and 54 were without metabolic syndrome (controls). Leptin and TNF-α mRNA expression in visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) were done by Real Time-RT PCR. In cases, the mean (±SD) serum estrogen was significantly lower (41.33±24.90 vs. 23.95±14.45, p<0.001) while leptin (12.85±4.51 vs. 10.34±3.89, p=0.002) and TNF-α (13.81±7.13 vs. 8.00±4.38, p<0.001) were significantly higher as compared to controls. Further, the mean relative VAT mRNA expression of both leptin (0.33±0.29 vs. 0.05±0.09, p<0.001) and TNF-α (0.32±0.31 vs. 0.13±0.09, p<0.001) and expression of SAT leptin (4.91±4.01 vs. 0.50±0.92, p<0.001) also lowered significantly in cases as compared to controls. Further, the relative VAT expression of both leptin (r=-0.32, p<0.001) and TNF-α (r=-0.23, p<0.01) showed significant and negative correlation with glucose; expression of SAT leptin showed significant and positive correlation with HDL (r=0.20, p<0.05) and serum estrogen (r=0.30, p<0.01) while negative correlation with glucose (r=-0.26, p<0.01) and serum TNF-α (r=-0.29, p<0.01); and expression of SAT TNF-α showed significant and positive correlation with insulin (r=0.21, p<0.05) and HOMA (r=0.20, p<0.05). In conclusion, the VAT and SAT leptin mRNA expressions may have a modulatory role in metabolic syndrome.
authors.affiliation: Department of Physiology, King George's Medical University, Lucknow, India.
authors.forename: S
authors.initials: S
authors.lastname: Tiwari
authors.affiliation: Immunobiology Division, Indian Institute of Toxicological Research, Lucknow, India.
authors.forename: B N
authors.initials: BN
authors.lastname: Paul
authors.affiliation: Director AIIMS, Bhopal, Madhya Pradesh, India.
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Department of Gastroenterology, King George's Medical University, Lucknow, India.
authors.forename: A
authors.initials: A
authors.lastname: Chandra
authors.affiliation: Department of Physiology, King George's Medical University, Lucknow, India.
authors.forename: S
authors.initials: S
authors.lastname: Dhananjai
authors.affiliation: Institute for Data Computing and Training, Lucknow, India.
authors.forename: M P S
authors.initials: MP
authors.lastname: Negi
mesh_terms: D000368:Aged; D001786:Blood Glucose / Q000032:analysis; D004967:Estrogens / Q000097:blood; D005260:Female; D015870:Gene Expression; D006801:Humans; D007194:India; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000502:physiology*; D050152:Intra-Abdominal Fat / Q000737:chemistry / Q000378:metabolism; D020738:Leptin / Q000097:blood / Q000235:genetics*; D024821:Metabolic Syndrome / Q000378:metabolism*; D008875:Middle Aged; D017698:Postmenopause; D012333:RNA, Messenger / Q000032:analysis; D050151:Subcutaneous Fat / Q000737:chemistry / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000235:genetics*
chemical_list: D001786:Blood Glucose; D004967:Estrogens; D007328:Insulin; D020738:Leptin; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha