title: The synthesis of a carbon-14 labeled pegylated Adnectin™ for placental transfer studies in guinea pigs.
abstract: Adnectins™ are novel fibronectin-based proteins containing domains engineered to bind to targets of therapeutic interest. The molecular weights of adnectins are less than conventional monoclonal antibodies but larger than traditional small molecules. Until now, there has been no information on the placental transfer of adnectins. To assess placental permeability to adnectins in pregnant guinea pigs, a radiolabeled adnectin, ATI-1072, bound to polyethylene glycol through a [(14) C]Maleimide linker, was synthesized from [1,4-(14) C]Maleic acid. This publication describes the synthesis and analysis of PEG-[(14) C]Maleimide-adnectin ([(14) C]ATI-1072).
authors.affiliation: Bristol-Myers Squibb Inc., Princeton, NJ, 08543, USA.
authors.forename: Brad D
authors.initials: BD
authors.lastname: Maxwell
authors.forename: Kai
authors.initials: K
authors.lastname: Cao
authors.forename: Samuel
authors.initials: S
authors.lastname: Bonacorsi
authors.forename: Lakshmi
authors.initials: L
authors.lastname: Sivaraman
authors.forename: Bonnie
authors.initials: B
authors.lastname: Wang
authors.forename: MaryEllen
authors.initials: M
authors.lastname: McNerney
authors.forename: Lifei
authors.initials: L
authors.lastname: Wang
authors.forename: Lisa J
authors.initials: LJ
authors.lastname: Christopher
authors.forename: Nga Kit Eliza
authors.initials: NK
authors.lastname: Fung
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Mark E
authors.initials: ME
authors.lastname: Arnold
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002250:Carbon Radioisotopes; D060326:Chemistry Techniques, Synthetic; D005260:Female; D005353:Fibronectins / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D006168:Guinea Pigs; D008301:Maleimides / Q000737:chemistry; D010920:Placenta / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011247:Pregnancy
chemical_list: D002250:Carbon Radioisotopes; D005353:Fibronectins; D008301:Maleimides; C043592:maleimide; D011092:Polyethylene Glycols
keywords: ATI-1072; adnectin; carbon-14; maleic acid; maleimide; polyethylene glycol