title: Synthesis, characterization and anticancer screening of some novel piperonyl-tetrazole derivatives.
abstract: A series of new 1,2-substituted tetrazole derivatives were synthesized and evaluated on MCF-7 (ER positive), MDA-MB-231 and ZR-75 (ER negative) breast cancer cell lines. Out of the fourteen compounds, three compounds 10, 12 and 14 showed higher inhibitory effects on MCF-7 cells. Whereas, compound 8 exhibited higher inhibition in MDA-MB-231 and ZR-75 cells at 10(-5) M concentration. Total RNA was extracted and effect of compounds on different marker genes was studied. The gene expression of CD44, BRAC and BAX were significantly affected. The compounds were screened against the HepG2 cell line, to know if they are selectively targeting specific cancers and only 1-10 percent inhibition was found at 10(-5) M concentration.
authors.affiliation: Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi 110025, India.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Arshad
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan 712-749, South Korea.
authors.forename: Abdul Roouf
authors.initials: AR
authors.lastname: Bhat
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan 712-749, South Korea.
authors.forename: Smritee
authors.initials: S
authors.lastname: Pokharel
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan 712-749, South Korea.
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Kim
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan 712-749, South Korea.
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi 110025, India. Electronic address: fathar@jmi.ac.in.
authors.forename: Fareeda
authors.initials: F
authors.lastname: Athar
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan 712-749, South Korea. Electronic address: inhochoi@ynu.ac.kr.
authors.forename: Inho
authors.initials: I
authors.lastname: Choi
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D001940:Breast / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D013777:Tetrazoles / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D013777:Tetrazoles; C045574:1H-tetrazole; D012313:RNA
keywords: Anticancer; MCF-7; MDA-MB-231; Tetrazole derivatives; ZR-75