title: Behaviour of rat-cultured dental pulp cells in three-dimensional collagen type-1 gel in vitro and in vivo.
abstract: The purpose of this study was to investigate the growth and differentiation potential of dental pulp cells (DPCs) in three-dimensional (3-D) collagen type-1 scaffold in vitro and in vivo. Third passage DPCs were cultured in a 3-D collagen and expression of both bone- or dentin-related mRNA (alkaline phosphatase (ALP), bone sialoprotein (BSP) and osteopontin (OPN)) and morphological changes evaluated in vitro. In the in vivo study, two types of grafts were transplanted into the rectus abdominus muscles of rats and harvested after 7 days: DPCs in α-minimal essential medium and DPCs mixed with a collagen gel. ALP, BSP and OPN were used as primary antibodies for immunohistochemical study. Histological and immunohistochemical results showed that DPCs in collagen gel were spindle shaped and showed significantly greater expression of ALP, BSP and OPN in vitro than the controls. Transplanted DPCs in collagen type-1 gel showed greater positive immunoreactivity for ALP, BSP and OPN than the controls. It was concluded that the collagen gel scaffold encouraged the differentiation of DPCs into osteoblastic cells.
authors.affiliation: Department of Clinical Pathophysiology, Tokyo Dental College, Chiba, Japan HRC-7, Tokyo Dental College, Chiba, Japan Department of Microbiology, Tokyo Dental College, Chiba, Japan.
authors.forename: Sultan Zeb
authors.initials: SZ
authors.lastname: Khan
authors.forename: Eitoyo
authors.initials: E
authors.lastname: Kokubu
authors.forename: Kenichi
authors.initials: K
authors.lastname: Matsuzaka
authors.forename: Takashi
authors.initials: T
authors.lastname: Inoue
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D048430:Cell Shape; D017690:Cell Transplantation / Q000379:methods; D002478:Cells, Cultured; D024042:Collagen Type I / Q000737:chemistry*; D003782:Dental Pulp / Q000166:cytology*; D058574:Integrin-Binding Sialoprotein / Q000032:analysis; D008297:Male; D010006:Osteoblasts / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology; D053495:Osteopontin / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D017568:Rectus Abdominis / Q000601:surgery; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D024042:Collagen Type I; C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D053495:Osteopontin; D000469:Alkaline Phosphatase
keywords: GFP rat; collagen type-1 gel; dental pulp cells; osteogenesis