title: Dynamics of the concentration of IAA and some of its conjugates during the induction of somatic embryogenesis in Coffea canephora.
abstract: Most of the somatic embryogenesis (SE) process requires the presence, either before or during the embryogenic process, of at least one exogenous auxin. This exogenous auxin induces the presence of endogenous auxins, which appears to be essential for SE induction. We found that during the preincubation period of SE in Coffea canephora, there is an important increase in both free and conjugated indole-3-acetic acid (IAA), as well as indole-3-butyric acid. This increase is accompanied by an increase in the expression of YUCCA (CcYUC), TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS 1 (CcTAA1), and GRETCHEN HAGEN 3 (GH3) genes. On the other hand, most of the IAA compounds decreased during the induction of SE. The results presented in this research suggest that a balance between free IAA and its amide conjugates is necessary to allow the expression of SE-related genes.
authors.affiliation: Unidad de Bioquímica y Biología Molecular de Plantas; Centro de Investigación Científica de Yucatán; Mérida, Yucatán, México.
authors.forename: Benajmín
authors.initials: B
authors.lastname: Ayil-Gutiérrez
authors.affiliation: Unidad de Bioquímica y Biología Molecular de Plantas; Centro de Investigación Científica de Yucatán; Mérida, Yucatán, México.
authors.forename: Rosa
authors.initials: R
authors.lastname: Galaz-Ávalos
authors.affiliation: Departamento de Química; Universidad de Guanajuato; Col. Noria Alta S/N, Guanajuato, México.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Peña-Cabrera
authors.affiliation: Unidad de Bioquímica y Biología Molecular de Plantas; Centro de Investigación Científica de Yucatán; Mérida, Yucatán, México.
authors.forename: Victor
authors.initials: V
authors.lastname: Loyola-Vargas
mesh_terms: D040503:Coffea / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D058439:Plant Somatic Embryogenesis Techniques; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D007210:Indoleacetic Acids; D010940:Plant Proteins; C030737:indoleacetic acid
keywords: Auxins; GH3; growth regulators; indole-3-acetic acid; somatic embryogenesis