title: Functional isoDNA aptamers: modified thrombin binding aptamers with a 2'-5'-linked sugar-phosphate backbone (isoTBA).
abstract: The regioisomeric 3'-deoxy-2'-5'-linked thrombin binding DNA aptamers (isoTBAs) were chemically synthesized and their ability to form unimolecular anti-parallel G-quadruplexes in the presence of K(+) ions was evaluated. These modified sequences retain the function of the native thrombin binding aptamer (TBA), exhibit better stability against exonuclease and are capable of slowing down the process of blood clotting.
authors.affiliation: Organic Chemistry Division, CSIR-National Chemical Laboratory, Dr Homi Bhabha Road, Pashan, Pune 411008, Maharashtra, India. va.kumar@ncl.res.in.
authors.forename: Anita D
authors.initials: AD
authors.lastname: Gunjal
authors.forename: Moneesha
authors.initials: M
authors.lastname: Fernandes
authors.forename: Namrata
authors.initials: N
authors.lastname: Erande
authors.forename: P R
authors.initials: PR
authors.lastname: Rajamohanan
authors.forename: Vaijayanti A
authors.initials: VA
authors.lastname: Kumar
mesh_terms: D052157:Aptamers, Nucleotide / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D054856:G-Quadruplexes; D007477:Ions / Q000737:chemistry; D011188:Potassium / Q000737:chemistry; D011485:Protein Binding; D013237:Stereoisomerism; D013403:Sugar Phosphates / Q000737:chemistry* / Q000378:metabolism; D013917:Thrombin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D052157:Aptamers, Nucleotide; D007477:Ions; D013403:Sugar Phosphates; C081676:thrombin aptamer; D013917:Thrombin; D011188:Potassium