title: Development of a new Sonovue™ contrast-enhanced ultrasound approach reveals temporal and age-related features of muscle microvascular responses to feeding.
abstract: Compromised limb blood flow in aging may contribute to the development of sarcopenia, frailty, and the metabolic syndrome. We developed a novel contrast-enhanced ultrasound technique using Sonovue™ to characterize muscle microvasculature responses to an oral feeding stimulus (15 g essential amino acids) in young (∼20 years) and older (∼70 years) men. Intensity-time replenishment curves were made via an ultrasound probe "fixed" over the quadriceps, with intermittent high mechanical index destruction of microbubbles within muscle vasculature. This permitted real-time measures of microvascular blood volume (MBV), microvascular flow velocity (MFV) and their product, microvascular blood flow (MBF). Leg blood flow (LBF) was measured by Doppler and insulin by enzyme-linked immunosorbent assay. Steady-state contrast concentrations needed for comparison between different physiological states were achieved <150 sec from commencing Sonovue™ infusion, and MFV and MBV measurements were completed <120 sec thereafter. Interindividual coefficients of variation in MBV and MFV were 35-40%, (N = 36). Younger men (N = 6) exhibited biphasic vascular responses to feeding with early increases in MBV (+36%, P < 0.008 45 min post feed) reflecting capillary recruitment, and late increases in MFV (+77%, P < 0.008) and MBF (+130%, P < 0.007 195 min post feed) reflecting more proximal vessel dilatation. Early MBV responses were synchronized with peak insulin but not increased LBF, while later changes in MFV and MBF occurred with insulin at post absorptive values but alongside increased LBF. All circulatory responses were absent in old men (N = 7). Thus, impaired postprandial circulation could impact age-related declines in muscle glucose disposal, protein anabolism, and muscle mass.
authors.affiliation: Division of Clinical Metabolic and Molecular Physiology, School of Graduate Entry Medicine and Health, University of Nottingham Derby, U.K. ; Department of Surgery, Royal Derby Hospital Derby, U.K.
authors.forename: William Kyle
authors.initials: WK
authors.lastname: Mitchell
authors.forename: Bethan E
authors.initials: BE
authors.lastname: Phillips
authors.forename: John P
authors.initials: JP
authors.lastname: Williams
authors.forename: Debbie
authors.initials: D
authors.lastname: Rankin
authors.forename: Kenneth
authors.initials: K
authors.lastname: Smith
authors.forename: Jonathan N
authors.initials: JN
authors.lastname: Lund
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Atherton
keywords: Aging; CEUS; microcirculation; muscle; nutrition