title: Subcellular localization of acid proteinase in barley mesophyll protoplasts.
abstract: Chloroplasts prepared from lysed protoplasts of barley mesophyll contain 2-8% of the total acid proteinase activity. This residual activity is not associated with intact chloroplasts isolated by means of density gradient centrifugation. Vacuoles isolated from lysed protoplasts contain 80-85% of the total acid proteinase activity, indicating that the enzyme(s) which is presumably responsible for the degradation of chloroplastic proteins is located largely in the central vacuoles of mesophyll cells.
authors.affiliation: Laboratorium für Pflanzenphysiologie der E.T.H. Zürich, Institut für Allgemeine Botanik, Sonneggstraße 5, CH-8006, Zürich, Switzerland.
authors.forename: U
authors.initials: U
authors.lastname: Heck
authors.forename: E
authors.initials: E
authors.lastname: Martinoia
authors.forename: P
authors.initials: P
authors.lastname: Matile