title: Multiscale immunomagnetic enrichment of circulating tumor cells: from tubes to microchips.
abstract: We review the rare cancer cell sorting technologies, with a focus on multiscale immunomagnetic approaches. Starting from the conventional magnetic activated cell sorting system, we derive the scaling laws of immunomagnetic assay and justify the recent trend of using downscaled systems for CTC studies. Furthermore, we introduce recent work on combining the immunomagnetic assay with microfluidic technology for enhanced separation. We summarize different types of in-channel micro-magnetic structures that can further increase the local magnetic field without lowering the system throughput. Related design concepts, principles, and microfabrication techniques are presented and evaluated.
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, Austin, Texas, USA. john.zhang@engr.utexas.edu.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.forename: Yu-Yen
authors.initials: YY
authors.lastname: Huang
authors.forename: Kazunori
authors.initials: K
authors.lastname: Hoshino
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000906:Antibodies / Q000276:immunology; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007118:Immunoassay; D008280:Magnetics; D046210:Microfluidic Analytical Techniques; D009360:Neoplastic Cells, Circulating / Q000276:immunology
chemical_list: D000906:Antibodies