title: Endomembrane and Golgi traffic in plant cells.
abstract: Leaf epidermal cells make ideal specimens for the investigation of the plant secretory pathway in that it is relatively easy to tag with fluorescent proteins and visualize in vivo the various organelles of the pathway. A number of techniques can be employed to identify and study proteins within the endomembrane organelles and to study their dynamics and interactions. Here, we discuss the most commonly used approaches to express proteins within arabidopsis and tobacco leaves, the use of mutant screens to identify trafficking proteins, and the use of two in vivo techniques, Fluorescence recovery after photobleaching and Förster resonance energy transfer, to study protein dynamics in plant cells.
authors.affiliation: Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan, USA; Department of Plant Biology, Michigan State University, East Lansing, Michigan, USA.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Stefano
authors.forename: Anne
authors.initials: A
authors.lastname: Osterrieder
authors.forename: Chris
authors.initials: C
authors.lastname: Hawes
authors.forename: Federica
authors.initials: F
authors.lastname: Brandizzi
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D006056:Golgi Apparatus / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D007425:Intracellular Membranes / Q000378:metabolism*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D018515:Plant Leaves / Q000166:cytology / Q000378:metabolism*; D021381:Protein Transport; D055571:Secretory Pathway; D014026:Nicotiana / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D014170:Transformation, Genetic
chemical_list: D029681:Arabidopsis Proteins; D049452:Green Fluorescent Proteins
keywords: FRET/FLIM; Fluorescent proteins; Golgi; Leaves; Mutant screening; Photobleaching; Plant secretory pathway; Transient expression