title: Remodeling of energy metabolism and absence of electrophysiological changes in the heart of obese hyperleptinemic mice. New insights into the pleiotropic role of leptin.
abstract: Dietary treatment with high-fat diets (HFD) triggers diabetes and hyperleptinemia, concomitantly with a partial state of leptin resistance that affects hepatic and adipose tissue but not the heart. In this context, characterized by widespread steatosis, cardiac lipid content remains unchanged. As previously reported, HFD-evoked hyperleptinemia could be a pivotal element contributing to increase fatty-acid (FA) metabolism in the heart and to prevent cardiac steatosis. This metabolic adaptation might theoretically reduce energy efficiency in cardiomyocytes and lead to cardiac electrophysiological remodeling. Therefore the aim of the current study has been to investigate the impact of long-term HFD on cardiac metabolism and electrophysiological properties of the principal ionic currents responsible of the action potential duration in mouse cardiomyocytes. Male C57BL/6J mice were fed a control (10 kcal% from fat) or HFD (45 kcal% from fat) during 32 weeks. Quantification of enzymatic activities regulating mitochondrial uptake of pyruvate and FA showed an increase of both carnitine-palmitoyltransferase and citrate synthase activities together with a decrease of lactate dehydrogenase and pyruvate dehydrogenase activities. Increased expression of uncoupling protein-3, Mn-, and Cu/Zn-superoxide dismutases and catalase were also detected. Total glutathione/oxidized glutathione ratios were unaffected by HFD. These data suggest that HFD triggers adaptive mechanisms aimed at (i) facilitating FA catabolism, and (ii) preventing oxidative stress. All these changes did not affect the duration of action potentials in cardiomyocytes and only slightly modified electrocardiographic parameters.
authors.affiliation: Departamento de Ciencias Farmacéuticas y de la Salud, Facultad de Farmacia, Universidad CEU-San Pablo , Madrid , Spain.
authors.forename: Rocío
authors.initials: R
authors.lastname: Guzmán-Ruiz
authors.forename: Nieves
authors.initials: N
authors.lastname: Gómez-Hurtado
authors.forename: Marta
authors.initials: M
authors.lastname: Gil-Ortega
authors.forename: Beatriz
authors.initials: B
authors.lastname: Somoza
authors.forename: M Carmen
authors.initials: MC
authors.lastname: González
authors.forename: Isabel
authors.initials: I
authors.lastname: Aránguez
authors.forename: Miriam
authors.initials: M
authors.lastname: Martín-Ramos
authors.forename: Carmen
authors.initials: C
authors.lastname: González-Martín
authors.forename: Carmen
authors.initials: C
authors.lastname: Delgado
authors.forename: Marisol
authors.initials: M
authors.lastname: Fernández-Alfonso
authors.forename: Mariano
authors.initials: M
authors.lastname: Ruiz-Gayo
keywords: UCP3; cardiac metabolism; electrophysiology; leptin; obesity