title: The value of frozen cartilage tissues without cryoprotection for genetic conservation.
abstract: Animal tissues frozen without cryoprotection are thought to be inappropriate for use as a donor for somatic cell nuclear transfer (SCNT) studies. Cells in tissues that have been frozen without a cryoprotectant are commonly thought to be dead or to have lost genomic integrity. However, in this study we show that the frozen auricular cartilage tissues of anatolian buffalo contain a considerable number of viable healthy cells. The cells in auricular cartilage tissues are resistant to cryo-injury at -80°C. Primary cell cultures were established from defrosted ear tissues which were frozen without cryoprotectant. The growth and functional characteristics of primary cell cultures are characterized according to cell growth curve, cell cycle analysis, karyotype and GAG synthesis. The results indicate that frozen cartilage tissues could be valuable materials for the conservation of species and SCNT technology.
authors.affiliation: TUBITAK MRC-Genetic Engineering and Biotechnology Institute (GEBI), 41470 Gebze, Kocaeli, Turkey. Electronic address: gayecet@gmail.com.
authors.forename: Gaye
authors.initials: G
authors.lastname: Cetinkaya
authors.affiliation: TUBITAK MRC-Genetic Engineering and Biotechnology Institute (GEBI), 41470 Gebze, Kocaeli, Turkey. Electronic address: ibrahim.hatipoglu@tubitak.gov.tr.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Hatipoglu
authors.affiliation: Namık Kemal University, Faculty of Agriculture, Department of Agricultural Biotechnology, Tekirdağ, Turkey. Electronic address: sarat@nku.edu.tr.
authors.forename: Sezen
authors.initials: S
authors.lastname: Arat
mesh_terms: D000818:Animals; D002020:Buffaloes; D002356:Cartilage / Q000166:cytology* / Q000502:physiology; D002452:Cell Count; D002453:Cell Cycle / Q000235:genetics*; D002470:Cell Survival; D003247:Conservation of Natural Resources; D003609:Dactinomycin / Q000031:analogs & derivatives; D004423:Ear; D005615:Freezing; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D059785:Karyotype; D061251:Primary Cell Culture
chemical_list: D006025:Glycosaminoglycans; D003609:Dactinomycin; C025942:7-aminoactinomycin D
keywords: Biobanking; Cartilage; Conservation; Cryoinjury; Gene preservation