title: Degradation of dibenzofuran via multiple dioxygenation by a newly isolated Agrobacterium sp. PH-08.
abstract: OBJECTIVE
  To demonstrate the biodegradation of dibenzofuran (DF) and its structural analogs by a newly isolated Agrobacterium sp. PH-08.
  
  
  RESULTS
  To assess the biodegradation potential of newly isolated Agrobacterium sp. PH-08, various substrates were evaluated as sole carbon sources in growth and biotransformation experiments. ESI LC-MS/MS analysis revealed the presence of angular degrading by-products as well as lateral dioxygenation metabolites in the upper pathway. The metabolites in the lower pathway also were detected. In addition, the cometabolically degraded daughter compounds of DF-related compounds such as BP and dibenzothiophene (DBT) in dual substrate degradation were observed. Strain PH-08 exhibited the evidence of meta-cleavage pathway as confirmed by the activity and gene expression of catechol-2,3-dioxygenase.
  
  
  CONCLUSIONS
  Newly isolated bacterial strain, Agrobacterium sp. PH-08, grew well with and degraded DF via both angular and lateral dioxygenation as demonstrated by metabolites identified through ESI LC-MS/MS and GC-MS analyses. The other heterocyclic pollutants were also cometabolically degraded.
  
  
  CONCLUSIONS
  Few reports have described the complete degradation of DF by a cometabolic lateral pathway. Our study demonstrates the novel results that the newly isolated strain utilized the DF as a sole carbon source and mineralized it via multiple dioxygenation.
authors.affiliation: School of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Korea.
authors.forename: T T
authors.initials: TT
authors.lastname: Le
authors.forename: K
authors.initials: K
authors.lastname: Murugesan
authors.forename: I-H
authors.initials: IH
authors.lastname: Nam
authors.forename: J-R
authors.initials: JR
authors.lastname: Jeon
authors.forename: Y-S
authors.initials: YS
authors.lastname: Chang
mesh_terms: D060054:Agrobacterium / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001572:Benzofurans / Q000378:metabolism*; D001673:Biodegradation, Environmental; D050557:Catechol 2,3-Dioxygenase / Q000235:genetics / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D013876:Thiophenes / Q000378:metabolism
chemical_list: D001572:Benzofurans; D013876:Thiophenes; C023614:dibenzofuran; D050557:Catechol 2,3-Dioxygenase; C016366:dibenzothiophene
keywords: Agrobacterium sp. PH-08; angular and lateral dioxygenation; cometabolism; dibenzofuran