title: Dual-SMAD Inhibition/WNT Activation-Based Methods to Induce Neural Crest and Derivatives from Human Pluripotent Stem Cells.
abstract: The neural crest (NC) is a transient population of multipotent cells giving rise to the peripheral nervous system, skin pigmentation, heart, and facial mesenchyme. The broad cell fate potential of NC makes it an attractive cell fate to derive from human pluripotent stem cells (hPSCs) for exploring embryonic development, modeling disease, and generating cells for transplantation. Here, we discuss recent publications and methods for efficiently differentiating hPSCs into NC. We also provide methods to direct NC into two different terminal fates: melanocytes and sensory neurons.
authors.affiliation: Developmental Biology Program, Center for Stem Cell Biology, Sloan-Kettering Institute, 1275 York Ave, New York, NY, 10065, USA.
authors.forename: Stuart M
authors.initials: SM
authors.lastname: Chambers
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Mica
authors.forename: Gabsang
authors.initials: G
authors.lastname: Lee
authors.forename: Lorenz
authors.initials: L
authors.lastname: Studer
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Tomishima
mesh_terms: D000818:Animals; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D006801:Humans; D008544:Melanocytes / Q000166:cytology; D051379:Mice; D009432:Neural Crest / Q000166:cytology*; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000166:cytology; D051785:Smad Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051153:Wnt Proteins / Q000378:metabolism*
chemical_list: D051785:Smad Proteins; D051153:Wnt Proteins