title: Peptide-decorated polyvinyl alcohol/hyaluronan nanofibers for human induced pluripotent stem cell culture.
abstract: Realization of the full potential of human induced pluripotent stem cells (hiPSCs) in clinical applications requires development of well-defined conditions for their growth and differentiation. A novel fully defined polyvinyl alcohol/hyaluronan (PVA/HA) polysaccharide nanofiber was developed for hiPSCs culture in commercially available xeno-free, chemically defined medium. Vitronectin peptide (VP) was immobilized to PVA/HA nanofibers through NHS/EDC chemistry. The hiPSCs successfully grew and proliferated on the VP-decorated PVA/HA nanofibers, similar to those on Matrigel™. Such well-defined, xeno-free and safe nanofiber substrate that supports culture of hiPSCs will not only help to accelerate the translational perspectives of hiPSCs, but also provide a platform to investigate the cell-nanofiber interaction mechanisms that regulate stem cell proliferation and differentiation.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University, Beijing 100081, China; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100081, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Deng
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhang
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Zhao
authors.forename: Shangshang
authors.initials: S
authors.lastname: Liang
authors.forename: Anxiu
authors.initials: A
authors.lastname: Xu
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.forename: Feng
authors.initials: F
authors.lastname: Deng
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
authors.forename: Shicheng
authors.initials: S
authors.lastname: Wei
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D019096:Vitronectin / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D055767:Immobilized Proteins; D010446:Peptide Fragments; D019096:Vitronectin; D011142:Polyvinyl Alcohol; D006820:Hyaluronic Acid
keywords: Cell culture; Human induced pluripotent stem cells; Hyaluronan; Nanofibers; Polyvinyl alcohol