title: A new high-speed droplet-real-time polymerase chain reaction method can detect bovine respiratory syncytial virus in less than 10 min.
abstract: The polymerase chain reaction (PCR) has been widely used for diagnosis of infectious diseases of domestic animals. Rapid detection of respiratory pathogens of cattle is useful for making therapeutic decisions. Therefore, we developed a new genetic-based method called droplet-real-time PCR, which can detect bovine respiratory syncytial virus (BRSV) within 10 min. Our droplet-real-time PCR markedly reduced the reaction time of reverse transcription-PCR while maintaining the same sensitivity as conventional real-time PCR, and it can be used as a rapid assay for detection of BRSV. Furthermore, our method is potentially applicable for rapid diagnosis of almost all infectious diseases, including highly pathogenic avian influenza virus.
authors.affiliation: Division of Medical Seeds Promotion, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Uehara
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Matsuda
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Sugano
authors.forename: Takayuki
authors.initials: T
authors.lastname: Honda
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000821:virology*; D003948:Diagnostic Techniques, Respiratory System / Q000662:veterinary*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis / Q000662:veterinary*; D018112:Respiratory Syncytial Virus, Bovine / Q000235:genetics*