title: Highly sensitive fluorescent immunosensor for detection of influenza virus based on Ag autocatalysis.
abstract: A versatile, ultrasensitive immunosensor for detection of influenza virus was designed by combining silver nanoparticles (Ag NPs) labeled antibodies with indirect fluorescence. A new technology using Ag-S covalent binding was applied for antibody labeling. Influenza A (H1N1) virus, as a subtype of influenza A virus that was the most common cause of human influenza (flu), was acted as the target antigen using sandwich type-immunoreactions on the high binding ELISA plates. The antibody-labeled Ag NPs were then released by acid solution to produce Ag(+) which can catalyze o-phenylenediamine (OPDA) oxidation to produce fluorescence for highly sensitive detection. Under the optimal conditions, it shows good linear relationship between fluorescence intensity and the logarithm of the concentration of H1N1 over the range of 1.0×10(-12)-1.0×10(-8) g mL(-1) with a detection limit (LOD, 3σ) of 1.0×10(-13) g mL(-1). Results indicated that the proposed method give a good sensitivity and simple operation for detecting the influenza virus. This work also provided a promising potential for antigen detection by Ag NPs labeled, and the steps were easy to handle.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), Fujian Province Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, 350002 China; Department of Chemistry and Chemical Engineering, Minjiang University, Fuzhou, Fujian 350108 China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Li
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), Fujian Province Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, 350002 China.
authors.forename: Mei
authors.initials: M
authors.lastname: Hong
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), Fujian Province Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, 350002 China.
authors.forename: Bin
authors.initials: B
authors.lastname: Qiu
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), Fujian Province Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, 350002 China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Department of Chemistry and Chemical Engineering, Minjiang University, Fuzhou, Fujian 350108 China.
authors.forename: Yiting
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Hong Kong, China. Electronic address: zwcai@hkbu.edu.hk.
authors.forename: Zongwei
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), Fujian Province Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, 350002 China. Electronic address: gnchen@fzu.edu.cn.
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D015200:Fluoroimmunoassay / Q000379:methods*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis / Q000821:virology; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D010084:Oxidation-Reduction; D010655:Phenylenediamines / Q000737:chemistry; D012834:Silver / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D010655:Phenylenediamines; D012834:Silver; C034193:1,2-diaminobenzene
keywords: Fluorescent immunoassay; H1N1 Influenza virus; OPDA; Silver nanoparticles