title: Differential responses to genotoxic agents between induced pluripotent stem cells and tumor cell lines.
abstract: Given potential values of induced pluripotent stem (iPS) cells in basic biomedical research and regenerative medicine, it is important to understand how these cells regulate their genome stability in response to environmental toxins and carcinogens. The present study characterized the effect of Cr(VI), a well-known genotoxic agent and environmental carcinogen, on major molecular components of DNA damage response pathways in human iPS cells. We compared the effect of Cr(VI) on human iPS cells with two established cell lines, Tera-1 (teratoma origin) and BEAS-2B (lung epithelial origin). We also studied the effect of hydrogen peroxide and doxorubicin on modulating DNA damage responses in these cell types. We demonstrated that ATM and p53 phosphorylation is differentially regulated in human iPS cells compared with Tera-1 and BEAS-2B cells after exposure to various genotoxic agents. Moreover, we observed that inhibition of CK2, but not p38, promotes phosphorylation of p53S392 in iPS cells. Combined, our data reveal some unique features of DNA damage responses in human iPS cells.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Lu
authors.forename: Dazhong
authors.initials: D
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.forename: Yupo
authors.initials: Y
authors.lastname: Ma
authors.forename: Yongping
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China. zengwnxian@hotmail.com.
authors.forename: Wenxian
authors.initials: W
authors.lastname: Zeng
authors.forename: Wei
authors.initials: W
authors.lastname: Dai
mesh_terms: D045744:Cell Line, Tumor; D017608:Chromium Compounds / Q000633:toxicity; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000633:toxicity; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism; D009153:Mutagens / Q000633:toxicity*; D010766:Phosphorylation; D013724:Teratoma / Q000235:genetics / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D017608:Chromium Compounds; D009153:Mutagens; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D004317:Doxorubicin; D006861:Hydrogen Peroxide