title: Expression of class II cytokine genes in children's skin.
abstract: Immune regulation of the skin plays an important role in susceptibility and development of illnesses. The aim of our study was to localise the interleukin (IL)-10 family of cytokines, in children's skin and to determine possible age-related differences in the expression level. The mRNA expression level of IL10, IL19, IL20, IL22, IL24, IL26, IL28B, IL29 and their receptors IL10RA, IL10RB, IL20RA, IL20RB, IL22RA1, IL22RA2, IL28RA was compared in skin biopsies of children and adults and in childrens' skin cells by quantitative real-time PCR (qRT-PCR). Immunohistochemistry was performed to confirm the qRT-PCR findings. We found age-related differences in the expression of IL10RB, IL20, IL20RA, IL22RA1, IL22RA2, IL26 and IL28RA genes. Cell type-dependent expression of IL10 family cytokines was apparent in the skin. In addition to previously known differences in systemic immunological response of adults and children, the present results reveal differences in immune profile of adult and juvenile skin.
authors.affiliation: Department of Physiology, University of Tartu, Ravila 19, 50411 Tartu, Estonia. paula.reemann@gmail.com, paula.reemann@ut.ee.
authors.forename: Paula
authors.initials: P
authors.lastname: Reemann
authors.forename: Ene
authors.initials: E
authors.lastname: Reimann
authors.forename: Siim
authors.initials: S
authors.lastname: Suutre
authors.forename: Maarjaliis
authors.initials: M
authors.lastname: Paavo
authors.forename: Ulvi
authors.initials: U
authors.lastname: Loite
authors.forename: Orm
authors.initials: O
authors.lastname: Porosaar
authors.forename: Kristi
authors.initials: K
authors.lastname: Abram
authors.forename: Helgi
authors.initials: H
authors.lastname: Silm
authors.forename: Eero
authors.initials: E
authors.lastname: Vasar
authors.forename: Sulev
authors.initials: S
authors.lastname: Kõks
authors.forename: Külli
authors.initials: K
authors.lastname: Kingo
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D018123:Receptors, Interleukin / Q000235:genetics / Q000378:metabolism*; D012867:Skin / Q000276:immunology*; D055815:Young Adult
chemical_list: D007378:Interleukins; D012333:RNA, Messenger; D018123:Receptors, Interleukin