title: Utilization of dental pulp DNA as diagnostic molecular marker for fertility detection in men.
abstract: Recent advances in DNA technology have revolutionized forensic identification procedures. Teeth dentin and pulp are rich sources of DNA material, which can be successfully extracted and it provides us with valuable information on individuals, systemic health including fertility status.
  
  
  OBJECTIVE
  The aim of this study was to use DNA material extracted from human teeth pulp for detection of fertility status of men.
  
  
  METHODS
  Twenty extracted premolar teeth of systemic disease free male Saudi individuals (45 years average age) were collected; eight of them were infertile while others were fertile and were used as control group. This information was concealed until the PCR analysis was performed. The results of recorded patient information was matched with the results of the DNA analysis.
  
  
  RESULTS
  Results showed that the gene (sY83) an important gene of AZFa region in Y chromosome is important for male fertility. It was later evident that the infertile patients suffered from azoospermia, and that information is completely matched with our results.
  
  
  CONCLUSIONS
  Using DNA extracted from dental pulp can be used successfully in determining fertility status of human which may help in an accurate personal identification specially in extreme circumstances.
authors.affiliation: Associate Professor, Department of Oral Medicine, Oral Diagnosis and Periodontology, Faculty of Dentistry, Suez Canal University, Ismailia Egypt, Phone: 966530834985, e-mail: Hossam_eid73@yahoo.com Dentaleagle2011@gmail.com.
authors.forename: Hossam A
authors.initials: HA
authors.lastname: Eid
authors.forename: Tarek H
authors.initials: TH
authors.lastname: Taha
authors.forename: Manea M
authors.initials: MM
authors.lastname: Alahmari
authors.forename: Abdullah Awn S
authors.initials: AA
authors.lastname: Alqarni
authors.forename: Abdulkarim Ali H
authors.initials: AA
authors.lastname: Alshehri
mesh_terms: D053713:Azoospermia / Q000235:genetics; D015415:Biomarkers / Q000032:analysis; D041322:Chromosomes, Human, Y / Q000235:genetics; D004247:DNA / Q000032:analysis*; D000075364:Deleted in Azoospermia 1 Protein; D003782:Dental Pulp / Q000737:chemistry*; D005298:Fertility / Q000235:genetics*; D017353:Gene Deletion; D056426:Genetic Loci; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D016601:RNA-Binding Proteins / Q000235:genetics; D013091:Spermatogenesis / Q000235:genetics
chemical_list: D015415:Biomarkers; C095668:DAZ1 protein, human; D000075364:Deleted in Azoospermia 1 Protein; D016601:RNA-Binding Proteins; D004247:DNA