title: Ribosomal Database Project: data and tools for high throughput rRNA analysis.
abstract: Ribosomal Database Project (RDP; http://rdp.cme.msu.edu/) provides the research community with aligned and annotated rRNA gene sequence data, along with tools to allow researchers to analyze their own rRNA gene sequences in the RDP framework. RDP data and tools are utilized in fields as diverse as human health, microbial ecology, environmental microbiology, nucleic acid chemistry, taxonomy and phylogenetics. In addition to aligned and annotated collections of bacterial and archaeal small subunit rRNA genes, RDP now includes a collection of fungal large subunit rRNA genes. RDP tools, including Classifier and Aligner, have been updated to work with this new fungal collection. The use of high-throughput sequencing to characterize environmental microbial populations has exploded in the past several years, and as sequence technologies have improved, the sizes of environmental datasets have increased. With release 11, RDP is providing an expanded set of tools to facilitate analysis of high-throughput data, including both single-stranded and paired-end reads. In addition, most tools are now available as open source packages for download and local use by researchers with high-volume needs or who would like to develop custom analysis pipelines.
authors.affiliation: Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA, Computer Science and Engineering, Michigan State University, East Lansing, MI 48824, USA, Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA, Biological Sciences, Western Illinois University, Malcomb, IL 61455, USA and Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
authors.forename: James R
authors.initials: JR
authors.lastname: Cole
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wang
authors.forename: Jordan A
authors.initials: JA
authors.lastname: Fish
authors.forename: Benli
authors.initials: B
authors.lastname: Chai
authors.forename: Donna M
authors.initials: DM
authors.lastname: McGarrell
authors.forename: Yanni
authors.initials: Y
authors.lastname: Sun
authors.forename: C Titus
authors.initials: CT
authors.lastname: Brown
authors.forename: Andrea
authors.initials: A
authors.lastname: Porras-Alfaro
authors.forename: Cheryl R
authors.initials: CR
authors.lastname: Kuske
authors.forename: James M
authors.initials: JM
authors.lastname: Tiedje
mesh_terms: D001105:Archaea / Q000145:classification; D001419:Bacteria / Q000145:classification; D030561:Databases, Nucleic Acid; D005658:Fungi / Q000145:classification; D019766:Genes, Archaeal; D005798:Genes, Bacterial; D005800:Genes, Fungal; D064351:Genes, Microbial; D020459:Genes, rRNA; D059014:High-Throughput Nucleotide Sequencing; D020407:Internet; D015345:Oligonucleotide Probes; D016415:Sequence Alignment; D012984:Software
chemical_list: D015345:Oligonucleotide Probes