title: Recent advances in the bioanalysis of modified nucleotides in epigenetic studies.
abstract: Epigenetic alterations, such as DNA methylation, are involved in the pathogenesis of various diseases, the toxicity of diverse agents, the process of aging, the development of stem cells and numerous other mechanisms. DNA methylation is one of the most well-studied epigenetic alterations in mammals. Nevertheless, the scientific interest is now focusing on novel modified nucleotides with potential regulatory roles, such as 5-hydroxymethylcytosine. We currently present and discuss novel bioanalytical strategies developed for the determination of various modified nucleotides in epigenetic studies.
authors.affiliation: Laboratory of Forensic Medicine & Toxicology, School of Medicine, Aristotle University of Thessaloniki, 54124, Greece.
authors.forename: Domniki
authors.initials: D
authors.lastname: Fragou
authors.forename: Aikaterini
authors.initials: A
authors.lastname: Fragou
authors.forename: Victoria
authors.initials: V
authors.lastname: Samanidou
authors.forename: Sofia
authors.initials: S
authors.lastname: Kouidou
authors.forename: Samuel
authors.initials: S
authors.lastname: Njau
authors.forename: Leda
authors.initials: L
authors.lastname: Kovatsi
mesh_terms: D044503:5-Methylcytosine / Q000031:analogs & derivatives; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D009711:Nucleotides / Q000032:analysis* / Q000235:genetics*
chemical_list: D009711:Nucleotides; C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA