title: High-throughput screening of small-molecule libraries for inducers of plant defense responses.
abstract: Transgenic Arabidopsis seedlings containing a pathogen-responsive reporter gene allow for convenient high-throughput screening of chemical libraries for compounds that induce plant defense responses. Candidates identified by such screens can be further tested for their ability to protect plants from pathogen-caused diseases. Using Arabidopsis defense signaling mutants, defined regulatory processes that are targeted by a given candidate molecule can be easily narrowed down. Here, we provide a detailed high-throughput screening protocol for library compounds that activate a pathogen-responsive reporter gene in liquid-grown Arabidopsis seedlings.
authors.affiliation: Focus Diagnostics, Cypress, CA, USA.
authors.forename: Colleen
authors.initials: C
authors.lastname: Knoth
authors.forename: Thomas
authors.initials: T
authors.lastname: Eulgem
mesh_terms: D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000276:immunology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017930:Genes, Reporter; D057166:High-Throughput Screening Assays; D010935:Plant Diseases / Q000517:prevention & control; D057865:Plant Immunity / Q000187:drug effects*; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000276:immunology; D036226:Seedlings / Q000187:drug effects* / Q000235:genetics / Q000276:immunology; D054852:Small Molecule Libraries
chemical_list: D029681:Arabidopsis Proteins; D054852:Small Molecule Libraries