title: Determination of free and total myo-inositol in infant formula and adult/pediatric nutritional formula by high- performance anion exchange chromatography with pulsed amperometric detection, including a novel total extraction using microwave-assisted acid hydrolysis and enzymatic treatment: first action 2012.12.
abstract: After an assessment of data generated from a single-laboratory validation study published in J. AOAC Int. 95, 1469-1478 (2012), a method for determining total myo-inositol in infant formula and adult/ pediatric nutritional formula by high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), including extraction by using microwave-assisted acid hydrolysis and enzymatic treatment was presented for consideration by AOAC during the AOAC Annual Meeting held in Las Vegas, NV, from September 30 to October 3, 2012. The Expert Review Panel on Infant Formula and Adult Nutritionals concluded that the method met the criteria set by the standard method performance requirements (SMPRs) for the determination of free myo-inositol and approved the method as AOAC Official First Action. The method also determines total myo-inositol, but includes bound sources that the SMPRs exclude. The method involves using HPAEC-PAD for free myo-inositol and a total myo-inositol determination by two different techniques. The first technique uses the conventional acid hydrolysis with 6 h incubation in an autoclave. The second uses a microwave-assisted acid hydrolysis with enzymatic treatment that decreases the extraction time.
authors.affiliation: Covance Laboratories, Nutritional Chemistry and Food Safety, 3301 Kinsman Blvd, Madison, WI 53704-2523, USA. David.Ellingson@covance.com
authors.forename: David
authors.initials: D
authors.lastname: Ellingson
authors.forename: Ted
authors.initials: T
authors.lastname: Pritchard
authors.forename: Pamela
authors.initials: P
authors.lastname: Foy
authors.forename: Kathryn
authors.initials: K
authors.lastname: King
authors.forename: Barbara
authors.initials: B
authors.lastname: Mitchell
authors.forename: John
authors.initials: J
authors.lastname: Austad
authors.forename: Doug
authors.initials: D
authors.lastname: Winters
authors.forename: Darryl
authors.initials: D
authors.lastname: Sullivan
authors.forename: Dawn
authors.initials: D
authors.lastname: Dowell
mesh_terms: D002852:Chromatography, Ion Exchange / Q000379:methods*; D055664:Electrochemical Techniques; D005526:Food, Formulated / Q000032:analysis*; D006868:Hydrolysis; D041943:Infant Formula / Q000737:chemistry*; D007294:Inositol / Q000032:analysis* / Q000302:isolation & purification; D008872:Microwaves
chemical_list: D007294:Inositol