title: Reciprocal responses in the interaction between Arabidopsis and the cell-content-feeding chelicerate herbivore spider mite.
abstract: Most molecular-genetic studies of plant defense responses to arthropod herbivores have focused on insects. However, plant-feeding mites are also pests of diverse plants, and mites induce different patterns of damage to plant tissues than do well-studied insects (e.g. lepidopteran larvae or aphids). The two-spotted spider mite (Tetranychus urticae) is among the most significant mite pests in agriculture, feeding on a staggering number of plant hosts. To understand the interactions between spider mite and a plant at the molecular level, we examined reciprocal genome-wide responses of mites and its host Arabidopsis (Arabidopsis thaliana). Despite differences in feeding guilds, we found that transcriptional responses of Arabidopsis to mite herbivory resembled those observed for lepidopteran herbivores. Mutant analysis of induced plant defense pathways showed functionally that only a subset of induced programs, including jasmonic acid signaling and biosynthesis of indole glucosinolates, are central to Arabidopsis's defense to mite herbivory. On the herbivore side, indole glucosinolates dramatically increased mite mortality and development times. We identified an indole glucosinolate dose-dependent increase in the number of differentially expressed mite genes belonging to pathways associated with detoxification of xenobiotics. This demonstrates that spider mite is sensitive to Arabidopsis defenses that have also been associated with the deterrence of insect herbivores that are very distantly related to chelicerates. Our findings provide molecular insights into the nature of, and response to, herbivory for a representative of a major class of arthropod herbivores.
authors.affiliation: Department of Biology, University of Western Ontario, London, Ontario, Canada N6A 5B7.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Zhurov
authors.forename: Marie
authors.initials: M
authors.lastname: Navarro
authors.forename: Kristie A
authors.initials: KA
authors.lastname: Bruinsma
authors.forename: Vicent
authors.initials: V
authors.lastname: Arbona
authors.forename: M Estrella
authors.initials: ME
authors.lastname: Santamaria
authors.forename: Marc
authors.initials: M
authors.lastname: Cazaux
authors.forename: Nicky
authors.initials: N
authors.lastname: Wybouw
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Osborne
authors.forename: Cherise
authors.initials: C
authors.lastname: Ens
authors.forename: Cristina
authors.initials: C
authors.lastname: Rioja
authors.forename: Vanessa
authors.initials: V
authors.lastname: Vermeirssen
authors.forename: Ignacio
authors.initials: I
authors.lastname: Rubio-Somoza
authors.forename: Priti
authors.initials: P
authors.lastname: Krishna
authors.forename: Isabel
authors.initials: I
authors.lastname: Diaz
authors.forename: Markus
authors.initials: M
authors.lastname: Schmid
authors.forename: Aurelio
authors.initials: A
authors.lastname: Gómez-Cadenas
authors.forename: Yves
authors.initials: Y
authors.lastname: Van de Peer
authors.forename: Miodrag
authors.initials: M
authors.lastname: Grbic
authors.forename: Richard M
authors.initials: RM
authors.lastname: Clark
authors.forename: Thomas
authors.initials: T
authors.lastname: Van Leeuwen
authors.forename: Vojislava
authors.initials: V
authors.lastname: Grbic
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D003517:Cyclopentanes / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D014644:Genetic Variation; D005961:Glucosinolates / Q000378:metabolism; D060434:Herbivory; D006790:Host-Parasite Interactions; D007814:Larva; D009154:Mutation; D054883:Oxylipins / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism; D015398:Signal Transduction; D040102:Tetranychidae / Q000235:genetics / Q000502:physiology*
chemical_list: D003517:Cyclopentanes; D005961:Glucosinolates; D054883:Oxylipins; D010937:Plant Growth Regulators; C011006:jasmonic acid