title: High-throughput, multiparameter analysis of single cells.
abstract: Heterogeneity of cell populations in various biological systems has been widely recognized, and the highly heterogeneous nature of cancer cells has been emerging with clinical relevance. Single-cell analysis using a combination of high-throughput and multiparameter approaches is capable of reflecting cell-to-cell variability, and at the same time of unraveling the complexity and interdependence of cellular processes in the individual cells of a heterogeneous population. In this review, analytical methods and microfluidic tools commonly used for high-throughput, multiparameter single-cell analysis of DNA, RNA, and proteins are discussed. Applications and limitations of currently available technologies for cancer research and diagnostics are reviewed in the light of the ultimate goal to establish clinically applicable assays.
authors.affiliation: Center for Advanced Bioanalysis GmbH, Gruberstraße 40-42, 4020, Linz, Austria, thomas.haselgruebler@cbl.at.
authors.forename: Thomas
authors.initials: T
authors.lastname: Haselgrübler
authors.forename: Michaela
authors.initials: M
authors.lastname: Haider
authors.forename: Bozhi
authors.initials: B
authors.lastname: Ji
authors.forename: Kata
authors.initials: K
authors.lastname: Juhasz
authors.forename: Alois
authors.initials: A
authors.lastname: Sonnleitner
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Balogi
authors.forename: Jan
authors.initials: J
authors.lastname: Hesse
mesh_terms: D000818:Animals; D005434:Flow Cytometry; D016678:Genome; D023281:Genomics; D006801:Humans; D008024:Ligands; D013058:Mass Spectrometry; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000379:methods; D044085:Microfluidics; D008853:Microscopy; D009362:Neoplasm Metastasis; D009369:Neoplasms / Q000175:diagnosis; D009696:Nucleic Acids / Q000032:analysis*; D011506:Proteins; D040901:Proteomics; D017423:Sequence Analysis, RNA; D059010:Single-Cell Analysis / Q000379:methods*; D059467:Transcriptome
chemical_list: D008024:Ligands; D009696:Nucleic Acids; D011506:Proteins