title: Development of a novel lipophilic, magnetic nanoparticle for in vivo drug delivery.
abstract: The aim of the present study was to evaluate the transfection potential of chitosan-coated, green-fluorescent magnetic nanoparticles (MNPs) (chi-MNPs) after encapsulation inside polyethylglycol (PEG)ylated liposomes that produced lipid-encapsulated chitosan-coated MNPs (lip-MNPs), and also to evaluate how these particles would distribute in vivo after systemic injection. The transfection potential of both chi-MNPs and lip-MNPs was evaluated in vitro in rat brain endothelial 4 (RBE4) cells with and without applying a magnetic field. Subsequently, the MNPs were evaluated in vivo in young rats. The in vitro investigations revealed that the application of a magnetic field resulted in an increased cellular uptake of the particles. The lip-MNPs were able to transfect the RBE4 cells with an incidence of approximately 20% of a commercial transfection agent. The in vivo distribution studies revealed that lip-MNPs had superior pharmacokinetic properties due to evasion of the RES, including hepatic Kuppfer cells and macrophages in the spleen. In conclusion, we were able to design a novel lipid-encapsulated MNP with the ability to carry genetic material, with favorable pharmacokinetic properties, and under the influence of a magnetic field with the capability to mediate transfection in vitro.
authors.affiliation: Section of Neurobiology, Biomedicine, Department of Health Science and Technology, Aalborg University, Fr. Bajers Vej 3B, 1.216, DK-9220 Aalborg East, Denmark. tmoos@hst.aau.dk.
authors.forename: Thomas
authors.initials: T
authors.lastname: Linemann
authors.forename: Louiza B
authors.initials: LB
authors.lastname: Thomsen
authors.forename: Kristian G du
authors.initials: KG
authors.lastname: Jardin
authors.forename: Jens C
authors.initials: JC
authors.lastname: Laursen
authors.forename: Jesper B
authors.initials: JB
authors.lastname: Jensen
authors.forename: Jacek
authors.initials: J
authors.lastname: Lichota
authors.forename: Torben
authors.initials: T
authors.lastname: Moos