title: Low expression of basic fibroblastic growth factor in mesenchymal stem cells and bone marrow of children with aplastic anemia.
abstract: BACKGROUND
  Our previous experiments with gene chip suggested that basic fibroblastic growth factor (FGF2) levels were lower in mesenchymal stem cell (MSC) from aplastic anemia patients. The purpose of this study was to determine the expression of FGF2 in MSC and in bone marrow of children with aplastic anemia to better understand the role of low FGF2 expression in the pathogenesis of aplastic anemia.
  
  
  METHODS
  MSCs from the bone marrow of aplastic anemia children and control group were cultured in vitro. Growth curves of primary and passage MSC were plotted. FGF2 gene expression in MSCs was detected using quantitative real-time polymerase chain reaction (RT-PCR). FGF2 protein expression in mononuclear cells and FGF2 protein level in extracellular fluid of bone marrow were also investigated.
  
  
  RESULTS
  Decreased growth of MSCs from aplastic anemia children was observed after passage 8 in serial subcultivation, and FGF2 gene expression was downregulated. Within the patients' bone marrow, low FGF2 expression was validated both in mononuclear cells and in the extracellular fluid.
  
  
  CONCLUSIONS
  Low FGF2 gene expression in MSCs and low FGF2 protein level in bone marrow of aplastic anemia may involve to pathogenesis of aplastic anemia.
authors.affiliation: Department of Hematology, Children's Hospital of Shanghai, Shanghai Jiao Tong University , Shanghai , China.
authors.forename: Sha yi
authors.initials: Sy
authors.lastname: Jiang
authors.forename: Xiao tian
authors.initials: Xt
authors.lastname: Xie
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.forename: Ji ji
authors.initials: Jj
authors.lastname: Zhou
authors.forename: Fu xing
authors.initials: Fx
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Cao
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D000741:Anemia, Aplastic / Q000235:genetics / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000378:metabolism* / Q000473:pathology; D002454:Cell Differentiation; D002478:Cells, Cultured; D002648:Child; D003114:Colony-Forming Units Assay; D015536:Down-Regulation; D045604:Extracellular Fluid / Q000737:chemistry; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology; D010006:Osteoblasts / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2