title: Rosmarinic acid prevents lipid peroxidation and increase in acetylcholinesterase activity in brain of streptozotocin-induced diabetic rats.
abstract: We investigated the efficacy of rosmarinic acid (RA) in preventing lipid peroxidation and increased activity of acetylcholinesterase (AChE) in the brain of streptozotocin-induced diabetic rats. The animals were divided into six groups (n = 8): control, ethanol, RA 10 mg/kg, diabetic, diabetic/ethanol and diabetic/RA 10 mg/kg. After 21 days of treatment with RA, the cerebral structures (striatum, cortex and hippocampus) were removed for experimental assays. The results demonstrated that the treatment with RA (10 mg/kg) significantly reduced the level of lipid peroxidation in hippocampus (28%), cortex (38%) and striatum (47%) of diabetic rats when compared with the control. In addition, it was found that hyperglycaemia caused significant increased in the activity of AChE in hippocampus (58%), cortex (46%) and striatum (30%) in comparison with the control. On the other hand, the treatment with RA reversed this effect to the level of control after 3 weeks. In conclusion, the present findings showed that treatment with RA prevents the lipid peroxidation and consequently the increase in AChE activity in diabetic rats, demonstrating that this compound can modulate cholinergic neurotransmission and prevent damage oxidative in brain in the diabetic state. Thus, we can suggest that RA could be a promising compound in the complementary therapy in diabetes.
authors.affiliation: Programa de Pós Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Santa Maria, RS, Brazil.
authors.forename: Nadia
authors.initials: N
authors.lastname: Mushtaq
authors.forename: Roberta
authors.initials: R
authors.lastname: Schmatz
authors.forename: Luciane B
authors.initials: LB
authors.lastname: Pereira
authors.forename: Mushtaq
authors.initials: M
authors.lastname: Ahmad
authors.forename: Naiara
authors.initials: N
authors.lastname: Stefanello
authors.forename: Juliano M
authors.initials: JM
authors.lastname: Vieira
authors.forename: Fátima
authors.initials: F
authors.lastname: Abdalla
authors.forename: Marília V
authors.initials: MV
authors.lastname: Rodrigues
authors.forename: Jucimara
authors.initials: J
authors.lastname: Baldissarelli
authors.forename: Luana Paula
authors.initials: LP
authors.lastname: Pelinson
authors.forename: Diéssica P
authors.initials: DP
authors.lastname: Dalenogare
authors.forename: Karine Paula
authors.initials: KP
authors.lastname: Reichert
authors.forename: Eduardo M
authors.initials: EM
authors.lastname: Dutra
authors.forename: Nádia
authors.initials: N
authors.lastname: Mulinacci
authors.forename: Marzia
authors.initials: M
authors.lastname: Innocenti
authors.forename: Maria
authors.initials: M
authors.lastname: Bellumori
authors.forename: Vera Maria
authors.initials: VM
authors.lastname: Morsch
authors.forename: Maria Rosa
authors.initials: MR
authors.lastname: Schetinger
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001921:Brain / Q000378:metabolism*; D002934:Cinnamates / Q000494:pharmacology*; D053630:Depsides / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D000097123:Rosmarinic Acid
chemical_list: D000110:Acetylcholinesterase; D000975:Antioxidants; D002934:Cinnamates; D053630:Depsides; D013311:Streptozocin; D000097123:Rosmarinic Acid
keywords: acetylcholinesterase; diabetes; lipid peroxidation; rosmarinic acid; streptozotocin