title: Assessment of the reproductive parameters, laparoscopic oocyte recovery and the first embryos produced in vitro from endangered Canindé goats (Capra hircus).
abstract: The Canindé breed of goats (Capra hircus) is currently endangered. The aims of this study were to characterize the estrus behavior, ovulatory responses and progesterone profiles, and to evaluate the in vitro embryo production (IVP) in this breed. In Experiment 1, ten nulliparous and seven pluriparous females received medroxyprogesterone acetate (MAP)-containing sponges (60mg) plus 75μg d-cloprostenol for estrus synchronization and their reproductive parameters were evaluated. In Experiment 2, oocytes obtained by laparascopy from hormonally stimulated females (n=15) were used for IVP. There was no difference (p>0.05) between nulliparous and pluriparous goats in terms of estrus response (40.0% vs. 85.7%), time from progestagen sponge removal to the onset of estrus (62.0±15.5 vs. 50.7±19.2h; mean±SEM), duration of estrus (25.0±16.1 vs. 30.0±15.1h), percentage of ovulating animals (60.0% vs. 85.7%), number of ovulations (1.2±0.4 vs. 1.3±0.8), and diameter of the preovulatory follicle (5.8±0.5 vs. 6.1±0.3mm). Progesterone concentrations were also similar (p>0.05) in both groups. During laparoscopic recovery, there were average 12.2 aspirated follicles and 9.1 oocytes per goat, resulting in a high recovery rate (74.3%, 182/245). A total of 78 embryos were produced (51.0%). The mean number of cells in the blastocysts at day 7 of in vitro culture was 170.3±12.5. In conclusion, nulliparous and pluriparous Canindé goats exhibited similar reproductive profiles. It was possible to produce embryos in vitro, allowing the instigation of an embryo bank for preservation of this breed.
authors.affiliation: Laboratory of Physiology and Control of Reproduction, School of Veterinary Medicine, Ceará State University, Av. Dedé Brasil 1700, Fortaleza, CE 60714-903, Brazil; INRA, UMR7247, Physiology of Reproduction and Behaviors, University of Tours, Haras Nationaux, Nouzilly 37380, France.
authors.forename: Joanna M G
authors.initials: JM
authors.lastname: Souza-Fabjan
authors.forename: Alexsandra F
authors.initials: AF
authors.lastname: Pereira
authors.forename: Carlos H S
authors.initials: CH
authors.lastname: Melo
authors.forename: Deisy J D
authors.initials: DJ
authors.lastname: Sanchez
authors.forename: Eunice
authors.initials: E
authors.lastname: Oba
authors.forename: Pascal
authors.initials: P
authors.lastname: Mermillod
authors.forename: Luciana M
authors.initials: LM
authors.lastname: Melo
authors.forename: Dárcio I A
authors.initials: DI
authors.lastname: Teixeira
authors.forename: Vicente J F
authors.initials: VJ
authors.lastname: Freitas
mesh_terms: D000818:Animals; D003247:Conservation of Natural Resources / Q000379:methods*; D056727:Endangered Species; D005260:Female; D006041:Goats / Q000502:physiology*; D059471:In Vitro Oocyte Maturation Techniques / Q000379:methods / Q000662:veterinary*; D010535:Laparoscopy / Q000662:veterinary*; D054315:Oocyte Retrieval / Q000379:methods / Q000662:veterinary*; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D012098:Reproduction / Q000502:physiology*
chemical_list: D011374:Progesterone
keywords: Conservation; Estrus; Goat; IVP; Oocyte recovery; Ovulation