title: Immunogenic activity of the fish tapeworm Pterobothrium heteracanthum (Trypanorhyncha: Pterobothriidae) in BALB/c mice.
abstract: The aim of this study was to verify the immunogenicity of Pterobothrium heteracanthum (Cestoda: Trypanorhyncha) crude protein extract (PH-CPE) in BALB/c mice. The parasites were obtained from Micropogonias furnieri (Osteichthyes: Sciaenidae). Groups of six mice were each immunized with 10, 50 or 100 μg of PH-CPE, on days 0 and 35. Both specific IgG and IgE responses were developed after immunization. The immunoblot assay revealed that specific IgG recognizes PH-CPE proteins with two molecular weight ranges, 60-75 and 30-40 kDa, and that IgE recognizes larger proteins over 120 kDa. This appears to be the first report on the immunogenicity of metacestodes within the Pterobothriidae and that PH-CPE is a potential inducer of a specific IgE response.
authors.affiliation: Programa de Pós-graduação em Medicina Veterinária, Faculdade de Medicina Veterinária, Universidade Federal Fluminense,Vital Brazil 64, Santa Rosa, Niterói - RJ 2423-340,Brazil.
authors.forename: D P B G
authors.initials: DP
authors.lastname: Mattos
authors.affiliation: Departamento de Imunobiologia,Instituto de Biologia, Universidade Federal Fluminense, Outeiro São João Batista,Centro, Niterói - RJ 24210-150,Brazil.
authors.forename: M A
authors.initials: MA
authors.lastname: Verícimo
authors.affiliation: Laboratório de Inspeção e Tecnologia de Pescado, Faculdade de Veterinária, Universidade Federal Fluminense,Vital Brazil 64, Santa Rosa, Niterói - RJ 24230-340,Brazil.
authors.forename: L M S
authors.initials: LM
authors.lastname: Lopes
authors.affiliation: Programa de Pós-graduação em Medicina Veterinária, Faculdade de Medicina Veterinária, Universidade Federal Fluminense,Vital Brazil 64, Santa Rosa, Niterói - RJ 2423-340,Brazil.
authors.forename: S C
authors.initials: SC
authors.lastname: São Clemente
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D002589:Cestoda / Q000235:genetics / Q000276:immunology*; D005393:Fish Diseases / Q000276:immunology / Q000469:parasitology*; D015801:Helminth Proteins / Q000276:immunology; D006725:Hookworm Infections / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D010473:Perciformes / Q000469:parasitology
chemical_list: D000909:Antibodies, Helminth; D015801:Helminth Proteins