title: A generic plant RNA isolation method suitable for RNA-Seq and suppression subtractive hybridization.
abstract: A recently developed revolutionary approach to transcriptomics, RNA-Seq, and suppression subtractive hybridization are powerful tools for gene expression research. However, currently, the difficulty of isolating high-quality RNAs from plant tissues bearing abundant complex polysaccharides, polyphenolics, and secondary metabolites is a serious problem that not only limits the application of these technologies but also hinders studies dealing with RNA in general. We have developed a consistent protocol to prepare highly intact and pure RNAs from tissues of a variety of field-grown plant species, with high yields, in 2 to 3 h. Additionally, this method can be readily applied to mammalian, yeast, and bacterial cells.
authors.affiliation: School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Zhu
authors.forename: W J
authors.initials: WJ
authors.lastname: Wu
authors.forename: H W
authors.initials: HW
authors.lastname: Xiao
authors.forename: H B
authors.initials: HB
authors.lastname: Chen
authors.forename: Y
authors.initials: Y
authors.lastname: Zheng
authors.forename: Y J
authors.initials: YJ
authors.lastname: Zhang
authors.forename: H X
authors.initials: HX
authors.lastname: Wang
authors.forename: L Q
authors.initials: LQ
authors.lastname: Huang
mesh_terms: D002458:Cell Fractionation / Q000379:methods; D005591:Chemical Fractionation / Q000379:methods; D009693:Nucleic Acid Hybridization; D059828:Plant Cells / Q000737:chemistry; D018749:RNA, Plant / Q000737:chemistry / Q000302:isolation & purification* / Q000592:standards; D017423:Sequence Analysis, RNA
chemical_list: D018749:RNA, Plant