title: Analysis of mixtures of fatty acids and fatty alcohols in fermentation broth.
abstract: Microbial production of fatty acids and fatty alcohols has attracted increasing concerns because of energy crisis and environmental impact of fossil fuels. Therefore, simple and efficient methods for the extraction and quantification of these compounds become necessary. In this study, a high-performance liquid chromatography-refractive index detection (HPLC-RID) method was developed for the simultaneous quantification of fatty acids and fatty alcohols in these samples. The optimum chromatographic conditions are C18 column eluted with methanol:water:acetic acid (90:9.9:0.1, v/v/v); column temperature, 26°C; flow rate, 1.0mL/min. Calibration curves of all selected analytes showed good linearity (r(2)≥0.9989). The intra-day and inter-day relative standard deviations (RSDs) of the 10 compounds were less than 4.46% and 5.38%, respectively, which indicated that the method had good repeatability and precision. Besides, a method for simultaneous extraction of fatty acids and fatty alcohols from fermentation broth was optimized by orthogonal design. The optimal extraction conditions were as follows: solvent, ethyl acetate; solvent to sample ratio, 0.5:1; rotation speed, 2min at 260rpm; extraction temperature, 10°C. This study provides simple and fast methods to simultaneously extract and quantify fatty acids and fatty alcohols for the first time. It will be useful for the study of microbial production of these products.
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Yilan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Chen
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Maohua
authors.initials: M
authors.lastname: Yang
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Wang
authors.affiliation: Huabei oilfield No. 1 middle school, Renqiu 062550, Cangzhou City, Hebei Province, China.
authors.forename: Weiyan
authors.initials: W
authors.lastname: Huo
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Daojiang
authors.initials: D
authors.lastname: Yan
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jinjin
authors.initials: J
authors.lastname: Chen
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jiemin
authors.initials: J
authors.lastname: Zhou
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: jmxing@home.ipe.ac.cn.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Xing
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000191:economics; D004926:Escherichia coli / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis*; D005233:Fatty Alcohols / Q000032:analysis*; D005285:Fermentation; D057230:Limit of Detection; D011550:Pseudomonas aeruginosa / Q000378:metabolism; D013997:Time Factors
chemical_list: D005227:Fatty Acids; D005233:Fatty Alcohols
keywords: Fatty acids; Fatty alcohols; Fermentation broth; HPLC-RID