title: Profiling of carbohydrate mixtures at unprecedented resolution using high-precision 1H-13C chemical shift measurements and a reference library.
abstract: Complex mixtures of carbohydrates pose distinct challenges in routine and high-throughput analysis, so that only a few carbohydrate components are routinely resolved and identified in biofluids, extracts, foods and other complex mixtures. Here, we conduct precise measurements of (1)H and (13)C anomeric chemical shifts to construct a reference library of specific carbohydrate signals with high-resolution two-dimensional (1)H-(13)C NMR spectra. High-resolution multidimensional NMR spectra largely abolish resolution problems in carbohydrate analysis with state-of-the-art instrumentation. Accurate measurements of anomeric (1)H-(13)C chemical shifts at parts per billion precisions permit robust carbohydrate identification using a very limited number of instrument-independent reference values.
authors.affiliation: Carlsberg Laboratory, Gamle Carlsberg Vej 10, 1799 Copenhagen V, Denmark. sebastian.meier@carlsberglab.dk.
authors.forename: Bent Ole
authors.initials: BO
authors.lastname: Petersen
authors.forename: Ole
authors.initials: O
authors.lastname: Hindsgaul
authors.forename: Sebastian
authors.initials: S
authors.lastname: Meier
mesh_terms: D002241:Carbohydrates / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000592:standards*; D012015:Reference Standards; D012995:Solubility
chemical_list: D002241:Carbohydrates