title: Optimization of ultrasonic-assisted extraction of continentalic acid from the root of Aralia continentalis by using the response surface methodology.
abstract: In order to optimize the extraction conditions of continentalic acid, the main compound from Aralia continentalis root, we developed a model using the response surface methodology. The continentalic acid yield was analyzed and quantified by high-performance liquid chromatography coupled with UV detection. The extraction solvent, temperature, and time the three main factors for ultrasound-assisted extraction were optimized using the central composite design. Analysis of variance showed a good model fit (R (2) = 0.9323). The maximum extraction of continentalic acid obtained experimentally was 1.0138 % under an extraction temperature of 33 °C and extraction time of 28 min when 100 % ethanol was used as the solvent. The experimental value was in good agreement (the yield 1.0103 %) with those predicted values. The results clearly showed that quality by statistical design could be effectively applied to optimize extraction of continentalic acid.
authors.affiliation: Herbal Medicine Resources Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Taejon, 305-811, Republic of Korea.
authors.forename: Hyo Seon
authors.initials: HS
authors.lastname: Kim
authors.forename: A Yeong
authors.initials: AY
authors.lastname: Lee
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Jo
authors.forename: Byeong Cheol
authors.initials: BC
authors.lastname: Moon
authors.forename: Yunui
authors.initials: Y
authors.lastname: Ji
authors.forename: Ho Kyoung
authors.initials: HK
authors.lastname: Kim
mesh_terms: D027983:Aralia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000302:isolation & purification*; D015394:Molecular Structure; D010936:Plant Extracts / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013010:Sonication / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D004224:Diterpenes; D010936:Plant Extracts; D012997:Solvents; C510689:continentalic acid