title: Sos recruitment system for the analysis of the interaction between sulfatase-modifying factor 2 subtypes and interleukin-13.
abstract: Interleukin (IL)-13 is a central mediator in allergic asthma. Our previous results have indicated that sulfatase-modifying factor 2 (SUMF2) interacts with IL-13 and inhibits its secretion. In this study, we investigated the interactions between SUMF2 subtypes and 2 types of IL-13. Wild type IL-13 (wh-IL-13) and its mutated counterpart (mh-IL-13) were analyzed and cloned into pSos yeast expression vectors. Protein was expressed in host cdc25H yeast strains. A quartet of agar growth plates was prepared for the yeast two-hybrid system, which was used to detect IL-13 and SUMF2 subtype interactions. Both yeast expression vectors, pSos/whIL-13 and pSos/whIL-13, and recombinant expression vectors for the 5 subtypes of SUMF2 (pMyr/SUMF2-Vx) were constructed. Our data showed that all of the SUMF2 subtypes bound to whIL-13 and mhIL-13 in the CytoTrap system. Five SUMF2 subtypes - SUMF2-V2, SUMF2-V3, SUMF2-V4, SUMF2-V5, and SUMF2-V7--interacted with whIL-13 and mhIL-13. These subtypes may contribute to allergic asthma by mediating IL-13 release.
authors.affiliation: Department of Clinical Biochemistry Laboratory, Fourth Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: W Y
authors.initials: WY
authors.lastname: Zhang
authors.forename: H Y
authors.initials: HY
authors.lastname: Liang
authors.forename: C
authors.initials: C
authors.lastname: Yang
authors.forename: L
authors.initials: L
authors.lastname: Xue
authors.forename: X F
authors.initials: XF
authors.lastname: Jiang
mesh_terms: D006801:Humans; D018793:Interleukin-13 / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011485:Protein Binding; D013429:Sulfatases / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: C578095:IL13 protein, human; D018793:Interleukin-13; C500803:SUMF2 protein, human; D013429:Sulfatases