title: Design and expression of a short peptide as an HIV detection probe.
abstract: To explore a low-cost novel probe for HIV detection, we designed and prepared a 50-amino acid-length short fusion peptide (FP-50) via Escherichia coli in vivo expression. It was employed as a novel probe to detect HIV-1 gp120 protein. The detectable level of gp120 protein using the FP-50 peptide was approximately 20-200 times lower than previously published methods that used a pair of monoclonal antibodies. Thus, this short peptide is a very promising component for detection of gp120 protein during early stages of HIV infection.
authors.affiliation: Center for BioEnergetics, Biodesign Institute, Arizona State University, Tempe, AZ 85287, United States.
authors.forename: Jamie A
authors.initials: JA
authors.lastname: Lines
authors.affiliation: Center for BioEnergetics, Biodesign Institute, Arizona State University, Tempe, AZ 85287, United States.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Center for BioEnergetics, Biodesign Institute, Arizona State University, Tempe, AZ 85287, United States.
authors.forename: Larisa M
authors.initials: LM
authors.lastname: Dedkova
authors.affiliation: Center for BioEnergetics, Biodesign Institute, Arizona State University, Tempe, AZ 85287, United States. Electronic address: shengxi.chen.1@asu.edu.
authors.forename: Shengxi
authors.initials: S
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D015699:HIV Envelope Protein gp120 / Q000097:blood*; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D015151:Immunoblotting; D015335:Molecular Probes / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics
chemical_list: C588187:FP-50 peptide; D015699:HIV Envelope Protein gp120; D015335:Molecular Probes; D011993:Recombinant Fusion Proteins; C514793:gp120 protein, Human immunodeficiency virus 1
keywords: Ab; CD4; ELISA; Early stages; FITC; Fusion peptide; HIV; HIV detection; In vivo expression; antibody; cluster of differentiation 4; enzyme-linked immunosorbent assay; fluorescein isothiocyanate; gp120; human immunodeficiency virus