title: Investigation of biological cell-protein interactions using SPR sensor through laser scanning confocal imaging-surface plasmon resonance system.
abstract: A new method for investigating biological cell-protein interactions was developed by using a laser scanning confocal imaging-surface plasmon resonance (LSCI-SPR) system. Mouse normal IgG was modified on the SPR chip. The suspension mouse lymphocyte cancer cells (L5178Y cells) labeled by Hoechst33342 freely flowed into the surface of the SPR sensor chip. By changing the concentration of the cells, the fluorescence images and the SPR signal were synchronously recorded in real time. The red fluorescence points in the imaging region increased with increase in the concentration of the mouse lymphocyte cancer cells and fit well with the change in the SPR signal. Different suspending cells were chosen to investigate cell-protein interactions through antigen-antibody reactions on the biological cell surfaces through binding detection. This method has potential application in cell biology and pharmacology.
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Liquan
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Bingjiang
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Xueliang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pediatrics, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China. Electronic address: liugvying@126.com.
authors.forename: Guiying
authors.initials: G
authors.lastname: Liu
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: wmliu@mail.ipc.ac.cn.
authors.forename: Weimin
authors.initials: W
authors.lastname: Liu
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Wang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002460:Cell Line; D002477:Cells / Q000378:metabolism*; D003199:Computer Systems; D005453:Fluorescence; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D007074:Immunoglobulin G
keywords: Biological cell–protein interactions; Laser scanning confocal imaging; Surface plasmon resonance