title: Characterisation and anti-biofilm activity of extracellular polymeric substances from Oceanobacillus iheyensis.
abstract: Oceanobacillus iheyensis BK6, isolated from a marine natural biofilm, produced approximately 40 0mg L(-1) extracellular polymeric substances (EPS). FTIR analysis of the EPS revealed different functional groups (halide groups, uronic acid and saccharides). The GCMS showed that the extracellular polysacchrides comprised of mannose (47.78%), glucose (29.71%) and arabinose (22.46%). The molecular mass of the EPS was about 2.14 × 10(6)Da. It was thermally stable and showed pseudoplastic rheology and emulsifying activity (66.47%). The EPS exhibited antibiofilm activity against a pathogenic strain of Staphylococcus aureus. This is the first report on the characterisation of EPS from the genus Oceanobacillus. The high viscosity, emulsifying properties and antibiofilm activity of EPS make it suitable for potential pharmaceutical and industrial applications.
authors.affiliation: Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar 364002, Gujarat, India; Academy of Scientific and Innovative Research, CSIR, New Delhi, India.
authors.forename: Kumari
authors.initials: K
authors.lastname: Kavita
authors.forename: Vijay Kumar
authors.initials: VK
authors.lastname: Singh
authors.forename: Avinash
authors.initials: A
authors.lastname: Mishra
authors.forename: Bhavanath
authors.initials: B
authors.lastname: Jha
mesh_terms: D001406:Bacillaceae / Q000737:chemistry* / Q000166:cytology / Q000302:isolation & purification; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D001704:Biopolymers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002241:Carbohydrates / Q000032:analysis; D005110:Extracellular Space / Q000737:chemistry*; D012212:Rheology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology; D013501:Surface-Active Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013696:Temperature
chemical_list: D001704:Biopolymers; D002241:Carbohydrates; D013501:Surface-Active Agents
keywords: Antibiofilm; Biofilm; EPS; Exopolymer; Marine bacteria