title: Xylitol production at high temperature by engineered Kluyveromyces marxianus.
abstract: Several recombinant Kluyveromyces marxianus strains were constructed through overexpressing the Neurospora crassa xylose reductase genes. YZJ015, which maintained the original xylitol dehydrogenase gene, produced xylitol with the highest productivity (1.49 g L(-1) h(-1)) from 100 g L(-1) xylose at 42 °C. Even at 45 °C, YZJ015 was still able to produce 60.03 g L(-1) xylitol from 100 g L(-1) xylose with a productivity of 1.25 g L(-1)h(-1). In addition, for 20 rounds of cell recycling at 42 °C, YZJ015 produced 71.35 g L(-1) xylitol from 100 g L(-1) xylose with a productivity of 4.43 g L(-1) h(-1) per cycle. YZJ017, in which the xylitol dehydrogenase gene was disrupted, produced 100.02 g L(-1) xylitol at a yield of 1.01 g g(-1) from 100 g L(-1) xylose with 40 g L(-1) glycerol as co-substrate at 42 °C. These engineered strains provide an excellent foundation for xylitol production at elevated temperatures.
authors.affiliation: School of Life Science, University of Science and Technology of China, Hefei, Anhui 230027, PR China; Hefei National Laboratory for Physical Science at the Microscale, Hefei, Anhui 230026, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Life Science, University of Science and Technology of China, Hefei, Anhui 230027, PR China; Hefei National Laboratory for Physical Science at the Microscale, Hefei, Anhui 230026, PR China.
authors.forename: Biao
authors.initials: B
authors.lastname: Zhang
authors.affiliation: School of Life Science, University of Science and Technology of China, Hefei, Anhui 230027, PR China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Wang
authors.affiliation: School of Life Science, University of Science and Technology of China, Hefei, Anhui 230027, PR China; Department of Biology and Biochemistry, University of Houston, Houston, TX 77004-5001, USA; Hefei National Laboratory for Physical Science at the Microscale, Hefei, Anhui 230026, PR China.
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Gao
authors.affiliation: School of Life Science, University of Science and Technology of China, Hefei, Anhui 230027, PR China; Hefei National Laboratory for Physical Science at the Microscale, Hefei, Anhui 230026, PR China. Electronic address: hjiong@ustc.edu.cn.
authors.forename: Jiong
authors.initials: J
authors.lastname: Hong
mesh_terms: D000449:Aldehyde Reductase / Q000235:genetics; D005285:Fermentation / Q000187:drug effects / Q000235:genetics; D018628:Gene Dosage; D005818:Genetic Engineering; D005947:Glucose / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D007716:Kluyveromyces / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D013696:Temperature; D014993:Xylitol / Q000096:biosynthesis*; D014994:Xylose / Q000378:metabolism
chemical_list: D014994:Xylose; D000449:Aldehyde Reductase; D005947:Glucose; D005990:Glycerol; D014993:Xylitol
keywords: Elevated temperature; Engineered Kluyveromyces marxianus; Xylitol; Xylose