title: Selective removal of keratan sulfate in chondroitin sulfate samples by sequential precipitation with ethanol.
abstract: Keratan sulfate (KS) is present as a contaminant in chondroitin sulfate (CS) mainly extracted from shark cartilage. We report a selective removal procedure of KS in CS samples by means of sequential precipitation with ethanol. Purified shark CS containing approximately 10% to 15% KS was subjected to a precipitation procedure in the presence of increasing percentages of saturated ethanol. In contrast to other solvents, 1.0 volume of ethanol was able to selectively purify CS, with a purity of approximately 100%, from KS. The current selective and simple procedure appears to be a reliable industrial preparation of CS devoid of large amounts of the residual KS.
authors.affiliation: Department of Life Sciences, University of Modena and Reggio Emilia, 41100 Modena, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Galeotti
authors.affiliation: Department of Life Sciences, University of Modena and Reggio Emilia, 41100 Modena, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Maccari
authors.affiliation: Department of Life Sciences, University of Modena and Reggio Emilia, 41100 Modena, Italy. Electronic address: volpi@unimo.it.
authors.forename: Nicola
authors.initials: N
authors.lastname: Volpi
mesh_terms: D000818:Animals; D002356:Cartilage / Q000737:chemistry / Q000378:metabolism; D011232:Chemical Precipitation; D002809:Chondroitin Sulfates / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry*; D007632:Keratan Sulfate / Q000302:isolation & purification*; D012754:Sharks / Q000378:metabolism; D012997:Solvents / Q000737:chemistry
chemical_list: D012997:Solvents; D000431:Ethanol; D002809:Chondroitin Sulfates; D007632:Keratan Sulfate
keywords: Capillary electrophoresis; Chondroitin sulfate; Ethanol precipitation; Glycosaminoglycans; Hexosamines; Keratan sulfate