title: Photopatternable and photoactive hydrogel for on-demand generation of hydrogen peroxide in cell culture.
abstract: Oxidative stress, largely mediated by reactive oxygen species (ROS), is a nearly ubiquitous component in complex biological processes such as aging and disease. Optimal in vitro methods used in elucidating disease mechanisms would deliver of low levels of hydrogen peroxide, emulating the in vivo pathological state, but current methods are limited by kinetic stability or accurate measurement of the dose administered. Here we present an in vitro platform that exploits anthraquinone catalysts for the photocatalytic production of hydrogen peroxide. This system can be dynamically tuned to provide constant generation of hydrogen peroxide at a desired physiologic rate over at least 14 days and is described using a kinetic model. Material characterization and stability is discussed along with a proof-of-concept in vitro study that assessed the viability of cells as they were oxidatively challenged over 24 h at different ROS generation rates.
authors.affiliation: Micro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California Davis, Davis, CA 95616, USA.
authors.forename: Shaun P
authors.initials: SP
authors.lastname: Garland
authors.affiliation: Micro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California Davis, Davis, CA 95616, USA.
authors.forename: Royal Y
authors.initials: RY
authors.lastname: Wang
authors.affiliation: Department of Surgical & Radiological Sciences, School of Veterinary Medicine, University of California Davis, Davis, CA 95616, USA.
authors.forename: Vijay Krishna
authors.initials: VK
authors.lastname: Raghunathan
authors.affiliation: Department of Biochemistry and Molecular Medicine, University of California Davis, Davis, CA 95616, USA.
authors.forename: Kit S
authors.initials: KS
authors.lastname: Lam
authors.affiliation: Department of Surgical & Radiological Sciences, School of Veterinary Medicine, University of California Davis, Davis, CA 95616, USA; Department of Ophthalmology & Vision Sciences, School of Medicine, University of California Davis, Davis, CA 95616, USA.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Murphy
authors.affiliation: Department of Surgical & Radiological Sciences, School of Veterinary Medicine, University of California Davis, Davis, CA 95616, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Russell
authors.affiliation: Division of Textiles and Clothing, University of California Davis, Davis, CA 95616, USA.
authors.forename: Gang
authors.initials: G
authors.lastname: Sun
authors.affiliation: Micro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California Davis, Davis, CA 95616, USA. Electronic address: tingrui@ucdavis.edu.
authors.forename: Tingrui
authors.initials: T
authors.lastname: Pan
mesh_terms: D002460:Cell Line; D020100:Hydrogels; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007700:Kinetics; D008954:Models, Biological; D018384:Oxidative Stress; D055668:Photochemical Processes
chemical_list: D020100:Hydrogels; D006861:Hydrogen Peroxide
keywords: Anthraquinone; Hydrogen peroxide; Oxidative stress; ROS