title: Catechol-O-methyltransferase Val158Met polymorphism (rs4680) is associated with pain in multiple sclerosis.
abstract: Alterations in the rs4680 Val158Met polymorphism are associated with the presence of pain. No study has investigated the role of Val158Met polymorphism in the susceptibility to exhibit pain in multiple sclerosis (MS). Our aim was to investigate the relationship between Val158Met polymorphism (rs4680) and the presence of pain in MS. One hundred eight (n = 108) patients (mean age: 44 ± 8 years) with a definitive diagnosis of MS and 108 matched controls participated. Fifty-eight patients (54%) had pain and 50 (46%) did not report pain. After amplifying Val158Met polymorphisms by polymerase chain reactions, rs4680 genotype frequencies and allele distributions were calculated. We classified individuals according to their Val158Met polymorphism: Val/Val, Val/Met, and Met/Met. The results showed that distribution of rs4680 Val158Met genotypes was not significantly different between individuals with MS in general and healthy people (χ2 = 2.212, P = .331). When we differentiate MS patients with pain and those without pain, the prevalence of Val158Met genotypes was significantly different (χ2 = 9,610, P = .046): Patients experiencing pain exhibited higher prevalence of Met/Met genotype than those without pain and healthy controls. Current results suggest that the Met allele of Val158Met polymorphism could be a potential risk factor for the development of pain in MS but not for the predisposition of MS itself.
  
  
  CONCLUSIONS
  This study suggests that the Val158Met polymorphism is associated with the presence of pain in MS, but it is not a risk factor for MS itself because the presence of the Met/Met genotype was more prevalent in those patients with pain. This study provides further evidence of potential genetic factors that predispose patients with MS to develop pain.
authors.affiliation: Department of Physical Therapy, Occupational Therapy, Rehabilitation and Physical Medicine, Universidad Rey Juan Carlos, Alcorcón, Madrid, Spain; Esthesiology Laboratory, Universidad Rey Juan Carlos, Alcorcón, Madrid, Spain. Electronic address: cesar.fernandez@urjc.es.
authors.forename: César
authors.initials: C
authors.lastname: Fernández-de-las-Peñas
authors.forename: Silvia
authors.initials: S
authors.lastname: Ambite-Quesada
authors.forename: Rosa
authors.initials: R
authors.lastname: Ortíz-Gutiérrez
authors.forename: Ricardo
authors.initials: R
authors.lastname: Ortega-Santiago
authors.forename: Antonio
authors.initials: A
authors.lastname: Gil-Crujera
authors.forename: Ana B
authors.initials: AB
authors.lastname: Caminero
mesh_terms: D000328:Adult; D000483:Alleles; D002394:Catechol O-Methyltransferase / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D008715:Methionine / Q000235:genetics*; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000175:diagnosis / Q000201:enzymology / Q000235:genetics*; D010146:Pain / Q000175:diagnosis / Q000201:enzymology / Q000235:genetics*; D010147:Pain Measurement / Q000379:methods; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors; D016037:Single-Blind Method; D014633:Valine / Q000235:genetics*
chemical_list: D008715:Methionine; C588010:COMT protein, human; D002394:Catechol O-Methyltransferase; D014633:Valine
keywords: Catechol-O-methyltransferase gene; multiple sclerosis; pain; polymorphism