title: Comparison between the organization of nuclear ribosomal DNA unit of Euglena gracilis Z and var. Bacillaris.
abstract: We have characterized the nuclear rDNA unit of Euglena gracilis var. bacillaris and compared it to that of the Z strain. We have localized restriction sites for Eco R1, Sal 1, Sma 1, Hind III, Bam H1 and Bgl II on this unit as well as the coding region for 20 S and 25 S rRNAs. For both strains, results suggest an homogeneity of the 11.6 kbp rDNA units. Comparison between strains shows differences characterized by two additional Sal 1 sites in bacillaris and the likely methylation of one Sma 1 site in Z. Both differences are localized in a non-coding region of the rDNA unit. Analyses of 18 Euglena strains from various origins confirm these differences and allow easy recognition of bacillaris and Z type strains.
authors.affiliation: Groupe de Recherche en Biochimie-Biologie Cellulaire, Université de limoges, 87060, Limoges Cédex, France.
authors.forename: O
authors.initials: O
authors.lastname: Neyret-Djossou
authors.forename: G
authors.initials: G
authors.lastname: Freyssinet
authors.forename: P
authors.initials: P
authors.lastname: Ravel-Chapuis
authors.forename: P
authors.initials: P
authors.lastname: Heizmann