title: Src regulates membrane trafficking of the Kv3.1b channel.
abstract: The Kv3.1 channel plays a crucial role in regulating the high-frequency firing properties of neurons. Here, we determined whether Src regulates the subcellular distributions of the Kv3.1b channel. Co-expression of active Src induced a dramatic redistribution of Kv3.1b to the endoplasmic reticulum. Furthermore, co-expression of the Kv3.1b channel with active Src induced a remarkable decrease in the pool of Kv3.1b at the cell surface. Moreover, the co-expression of active Src results in a significant decrease in the peak current densities of the Kv3.1b channel, and a substantial alteration in the voltage dependence of its steady-state inactivation. Taken together, these results indicate that Src kinase may play an important role in regulating membrane trafficking of Kv3.1b channels.
authors.affiliation: Department of Physiology, Kyung Hee University School of Medicine, Seoul 130-701, South Korea.
authors.forename: Seong Han
authors.initials: SH
authors.lastname: Bae
authors.affiliation: College of Pharmacy, Catholic University of Korea, Bucheon 420-743, Gyeonggi-Do, South Korea.
authors.forename: Dong Hyun
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Department of Physiology, Kyung Hee University School of Medicine, Seoul 130-701, South Korea.
authors.forename: Seok Kyo
authors.initials: SK
authors.lastname: Shin
authors.affiliation: College of Pharmacy, Catholic University of Korea, Bucheon 420-743, Gyeonggi-Do, South Korea.
authors.forename: Jin Sung
authors.initials: JS
authors.lastname: Choi
authors.affiliation: Department of Physiology, Kyung Hee University School of Medicine, Seoul 130-701, South Korea; Biomedical Science Institute, Kyung Hee University School of Medicine, Seoul 130-701, South Korea. Electronic address: kspark@khu.ac.kr.
authors.forename: Kang-Sik
authors.initials: KS
authors.lastname: Park
mesh_terms: D000818:Animals; D015153:Blotting, Western; D019556:COS Cells; D002462:Cell Membrane / Q000378:metabolism* / Q000502:physiology; D002522:Chlorocebus aethiops; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D016297:Mutagenesis, Site-Directed; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D018408:Patch-Clamp Techniques; D021381:Protein Transport; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D051670:Shaw Potassium Channels / Q000235:genetics / Q000378:metabolism* / Q000502:physiology
chemical_list: C098254:Kcnc1 protein, rat; D009419:Nerve Tissue Proteins; D051670:Shaw Potassium Channels; D016392:Proto-Oncogene Proteins pp60(c-src)
keywords: Kv3.1b; Membrane trafficking; Potassium channel; Src