title: Extraction, preliminary characterization and antioxidant activity of Se-enriched Maitake polysaccharide.
abstract: A Se-enriched Grifola frondosa polysaccharide (Se-GP) was obtained from G. frondosa enriched with Se by spraying an Na2SeO3 solution during fruit body growth using a Box-Behnken design and compared to G. frondosa polysaccharide (GP) for preliminary characterization and determination of the antioxidant activity. Under optimal conditions, polysaccharide yields and both the Se-GP and GP contents do not differ; however, the Se content of Se-GP (17.52 μg/g) was 48.7 times that of GP. Three homogenous Se-GPs (Se-GP11, Se-GP22 and Se-GP33) or GPs (GP11, GP22 and GP33) were obtained via DEAE-52 and Sephacryl S-400 purification. Their molecular weight and polysaccharide content of these compounds were not obviously different, whereas the Se content of Se-GP11, Se-GP22 and Se-GP33 was 9.41, 6.59 and 16.25 times that of GP11, GP22 and GP33, respectively. The antioxidant activity of Se-GP for the DPPH, ABTS and hydroxyl radicals was higher than that of GP and was highest for the hydroxyl radical.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, Jiangsu, China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Mao
authors.forename: Ye
authors.initials: Y
authors.lastname: Zou
authors.forename: Weiwei
authors.initials: W
authors.lastname: Feng
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Ting
authors.initials: T
authors.lastname: Zhao
authors.forename: Changwen
authors.initials: C
authors.lastname: Ye
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhu
authors.forename: Xueshan
authors.initials: X
authors.lastname: Wu
authors.forename: Liuqing
authors.initials: L
authors.lastname: Yang
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Wu
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D048691:Grifola / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D012643:Selenium / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D011134:Polysaccharides; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl; D012643:Selenium
keywords: Antioxidant; Box–Behnken; Polysaccharide; Purification; Se-enriched Grifola frondosa