title: Human umbilical cord-derived mesenchymal stem cells downregulate inflammatory responses by shifting the Treg/Th17 profile in experimental colitis.
abstract: OBJECTIVE
  The aim of this study was to evaluate the effect and mechanisms of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) on immune responses in murine colitis.
  
  
  METHODS
  Mice with dextran sulfate sodium (DSS)-induced colitis were injected intraperitoneally with hUC-MSCs or human bone marrow-derived MSCs. The cytokine levels from lamina propria mononuclear cells (LPMCs) and colon tissue were measured using ELISA. Treg and Th17 cells were analyzed using flow cytometry. The proliferation of LPMCs was assessed using Cell Counting Kit-8.
  
  
  RESULTS
  hUC-MSCs ameliorate DSS-induced colitis via the downregulation of colon inflammatory responses. Furthermore, hUC-MSCs adjusted modulation of Treg/Th17 cells in the spleen and mesenteric lymph nodes. hUC-MSCs also inhibited LPMCs in vitro.
  
  
  CONCLUSIONS
  hUC-MSCs may be an alternative source of stem cells and are worthy of study in long-term clinical trials.
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Liren
authors.initials: L
authors.lastname: Li
authors.forename: Suzhe
authors.initials: S
authors.lastname: Liu
authors.forename: Yue
authors.initials: Y
authors.lastname: Xu
authors.forename: Aixian
authors.initials: A
authors.lastname: Zhang
authors.forename: Jinxia
authors.initials: J
authors.lastname: Jiang
authors.forename: Wei
authors.initials: W
authors.lastname: Tan
authors.forename: Jing
authors.initials: J
authors.lastname: Xing
authors.forename: Guijuan
authors.initials: G
authors.lastname: Feng
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Fangxin
authors.initials: F
authors.lastname: Huo
authors.forename: Qiyun
authors.initials: Q
authors.lastname: Tang
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Gu
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D003092:Colitis / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D016207:Cytokines / Q000378:metabolism; D016264:Dextran Sulfate / Q000633:toxicity; D004195:Disease Models, Animal; D015536:Down-Regulation; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000628:therapy; D008198:Lymph Nodes / Q000276:immunology; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D009092:Mucous Membrane / Q000378:metabolism; D013154:Spleen / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology*; D014470:Umbilical Cord / Q000166:cytology
chemical_list: D016207:Cytokines; D016264:Dextran Sulfate