title: Lipid profiling of follicular fluid from women undergoing IVF: young poor ovarian responders versus normal responders.
abstract: This study identified possible lipid biomarkers in follicular fluid from women with poor ovarian response. These biomarkers indicate pathophysiological pathways and have potential diagnostic applications. An observational case-control study of young women undergoing ovarian stimulation for in-vitro fertilization was conducted. The participants were categorized into a poor ovarian response group and a normal ovarian response to stimulation group. All of the women underwent the same ovarian stimulation protocol, and follicular fluid was collected after ovarian aspiration. Analyses were performed using matrix-assisted laser desorption/ionization mass spectrometry. Principal component analysis and Volcano plots were used to describe follicular fluid classification models based on the lipid profiles. A total of 10 lipids were differentially expressed between the study and control groups. Of these lipid ions, three belonged to the phosphatidylcholine subclass and were present in higher concentrations in the control group. The other seven differential lipids were present in the study group and classified into four lipid subclasses: phosphatidylethanolamines, phosphatidylglycerols, phosphatidylinositols, and diacylglycerols. These distinctive lipids may be involved in hormonal responses and oocyte development processes and may be useful as biomarkers for therapeutic intervention in women with poor ovarian response.
authors.affiliation: Department of Surgery, Division of Urology, Human Reproduction Section, Sao Paulo Federal University , Sao Paulo, SP , Brazil.
authors.forename: Thais
authors.initials: T
authors.lastname: Cataldi
authors.forename: Fernanda Bertuccez
authors.initials: FB
authors.lastname: Cordeiro
authors.forename: Lívia do Vale Teixeira da
authors.initials: Ldo V
authors.lastname: Costa
authors.forename: Eduardo Jorge
authors.initials: EJ
authors.lastname: Pilau
authors.forename: Christina Ramires
authors.initials: CR
authors.lastname: Ferreira
authors.forename: Fábio Cesar
authors.initials: FC
authors.lastname: Gozzo
authors.forename: Marcos Nogueira
authors.initials: MN
authors.lastname: Eberlin
authors.forename: Ricardo Pimenta
authors.initials: RP
authors.lastname: Bertolla
authors.forename: Agnaldo Pereira
authors.initials: AP
authors.lastname: Cedenho
authors.forename: Edson Guimarães Lo
authors.initials: EG
authors.lastname: Turco
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000737:chemistry*; D006801:Humans; D008055:Lipids / Q000032:analysis*; D054315:Oocyte Retrieval; D010062:Ovulation Induction / Q000379:methods*; D010713:Phosphatidylcholines / Q000032:analysis*; D011247:Pregnancy; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017211:Treatment Failure
chemical_list: D008055:Lipids; D010713:Phosphatidylcholines