title: Identification of Outer Membrane Proteins Altered in Response to UVC-Radiation in Vibrio parahaemolyticus and Vibrio alginolyticus.
abstract: Vibrio parahaemolyticus and V. alginolyticus, marine foodborne pathogens, were treated with UVC-radiation (240 J/m(2)) to evaluate alterations in their outer membrane protein profiles. Outer membrane protein patterns of UVC-irradiated bacteria were found altered when analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Altered proteins were identified by mass spectrometry (MS and MS/MS) and analysis revealed that OmpW, OmpA, Long-chain fatty acid transport protein, Outer membrane receptor protein, Putative uncharacterized protein VP0167, Maltoporin (lamB), Polar flagellin B/D, Agglutination protein Peptidoglycan-associated lipoprotein and MltA-interacting protein MipA were appeared, thereby they can be considered as UVC-stress proteins in some vibrios. In addition, expression of OmpK decreased to non-detectable level. Furthermore, we observed a decrease or an increase in the expression level of other outer membrane proteins.
authors.affiliation: Laboratoire d'Analyse, Traitement et Valorisation des Polluants de l'Environnement et des Produits, Faculté de Pharmacie Rue Avicenne, 5000 Monastir, Tunisia ; Unité de Fermentation et de Développement de Vaccins Virologiques, Institut Pasteur de Tunis, 13 place Pasteur, 1002 Tunis, Tunisia.
authors.forename: Fethi Ben
authors.initials: FB
authors.lastname: Abdallah
authors.forename: Rihab
authors.initials: R
authors.lastname: Lagha
authors.forename: Ali
authors.initials: A
authors.lastname: Ellafi
authors.forename: Abdelkader
authors.initials: A
authors.lastname: Namane
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Rousselle
authors.forename: Pascal
authors.initials: P
authors.lastname: Lenormand
authors.forename: Héla
authors.initials: H
authors.lastname: Kallel
keywords: Alteration; Mass spectrometry; Outer membrane proteins; UVC; Vibrio