title: Elevated CD57 and CD95 expressions are associated with lower numbers of CD4⁺ recent thymic emigrants in HIV-1 infected immune responders following antiretroviral treatment.
abstract: The goal of this study was to understand how immune reconstitution through ART in HIV-1 infected patients affects CD4(+) recent thymic emigrants (identified as CD31(+) naïve T cells). We performed FACS analysis of CD4(+) CD31(+) naïve T cells from PBMCs in a cross-sectional age-matched cohort, including 25 healthy controls (HC), 18 untreated HIV-1 infected viremic progressors (VP), 10 untreated HIV-1 infected viral controllers (VC), and 24 HIV-1 infected immune responders (IR) following ART. Our data reveal that 37.5% of IR failed to restore their CD4(+) CD31(+) naïve T cell counts. In addition, significantly higher expressions of Ki67, CD57, and CD95 were observed in CD4(+) CD31(+) naïve T cells of both VP and IR comparing to HC and VC. The significantly elevated CD57 and CD95 expressions are observed in IR with low CD4(+) CD31(+) naïve T cell counts. Therefore, our data indicate an incomplete immune reconstitution of CD4(+) CD31(+) naïve T cells in more than one third of IR, which is associated with HIV-1 driven immunological phenotypic alterations.
authors.affiliation: Department of Clinical Immunology and Rheumatology, Hanover Biomedical Research School (HBRS), Hanover Medical University, Hanover, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Lu
authors.affiliation: Department of Clinical Immunology and Rheumatology, Hanover Biomedical Research School (HBRS), Hanover Medical University, Hanover, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Eberhard
authors.affiliation: Department of Clinical Immunology and Rheumatology, Hanover Biomedical Research School (HBRS), Hanover Medical University, Hanover, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Ahmad
authors.affiliation: Department of Clinical Immunology and Rheumatology, Hanover Biomedical Research School (HBRS), Hanover Medical University, Hanover, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Bhatnagar
authors.affiliation: Department of Clinical Immunology and Rheumatology, Hanover Biomedical Research School (HBRS), Hanover Medical University, Hanover, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Behrens
authors.affiliation: Department of Clinical Immunology and Rheumatology, Hanover Biomedical Research School (HBRS), Hanover Medical University, Hanover, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Jacobs
authors.affiliation: Department of Clinical Immunology and Rheumatology, Hanover Biomedical Research School (HBRS), Hanover Medical University, Hanover, Germany. Electronic address: immunologie@mh-hannover.de.
authors.forename: R E
authors.initials: RE
authors.lastname: Schmidt
authors.affiliation: Department of Clinical Immunology and Rheumatology, Hanover Biomedical Research School (HBRS), Hanover Medical University, Hanover, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Meyer-Olson
mesh_terms: D000328:Adult; D000368:Aged; D044966:Anti-Retroviral Agents / Q000627:therapeutic use*; D054316:Biomarkers, Pharmacological / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000821:virology; D018959:CD57 Antigens / Q000235:genetics / Q000378:metabolism*; D003430:Cross-Sectional Studies; D018450:Disease Progression; D005260:Female; D015658:HIV Infections / Q000188:drug therapy* / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000276:immunology / Q000821:virology; D013950:Thymus Gland / Q000276:immunology; D015854:Up-Regulation; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism*
chemical_list: D044966:Anti-Retroviral Agents; D054316:Biomarkers, Pharmacological; D018959:CD57 Antigens; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D019014:fas Receptor
keywords: HIV-1; Immune responders; Recent thymic emigrants