title: Cluster of erythrocyte band 3: a potential molecular target of exhaustive exercise-induced dysfunction of erythrocyte deformability.
abstract: The aim of this study is to explore the effect of exhaustive exercise on erythrocyte band 3 (SLC4A1; EB3). The association between the alterations of EB3 and red blood cell (RBC) deformability induced by exercise-induced dysfunction has been investigated. Rats were divided among 2 groups: (i) control (C), and (ii) exercise exhausted (E). RBC deformability was investigated in the rats in the exhaustive exercise and control groups. Erythrocytes from the control and exercise-exhausted groups were evaluated for the expression of erythrocyte band 3 through immunoblotting and immunofluorescence studies. Exhaustive exercise led to significant increments in the levels of clustering of erythrocyte band 3 along with the conjugation of membrane proteins to form high-molecular-weight complexes (P < 0.05). Under shear stresses, RBC deformability was found to decline significantly in the exhaustive exercise groups compared with the control group. These data suggest that the RBC dysfunction observed during exercise-induced oxidative stress could be associated with alterations in the structure and function of erythrocyte band 3, which in turn leads to dysfunction in the rheological properties of RBCs. These results provide further insight into erythrocyte damage induced by exhaustive exercise.
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, P.R. China.
authors.forename: Yanlian
authors.initials: Y
authors.lastname: Xiong
authors.forename: Yaojin
authors.initials: Y
authors.lastname: Li
authors.forename: Yanlei
authors.initials: Y
authors.lastname: Xiong
authors.forename: Yajin
authors.initials: Y
authors.lastname: Zhao
authors.forename: Fuzhou
authors.initials: F
authors.lastname: Tang
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism*; D000975:Antioxidants / Q000378:metabolism; D004907:Erythrocyte Deformability / Q000502:physiology*; D004910:Erythrocyte Membrane / Q000378:metabolism / Q000502:physiology; D004912:Erythrocytes / Q000378:metabolism / Q000502:physiology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D033081:Shear Strength / Q000502:physiology
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; D000975:Antioxidants; C534832:Mapre3 protein, rat; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins