title: Application of bone marrow and adipose-derived mesenchymal stem cells for testing the biocompatibility of metal-based biomaterials functionalized with ascorbic acid.
abstract: In this study, metal-based biomaterials were functionalized with ascorbic acid (LAA). Two types of substrates were used: austenitic steel 316L and titanium Ti6Al4V. Coatings were prepared with the sol-gel method and applied on metal surfaces using the dip-coating technique. Ascorbic acid was delivered with SiO2-coating at concentrations of 0.1 and 0.4 M. The morphology of the surfaces and coatings was determined using scanning electron microscope (SEM), whereas their elemental composition by SEM-EDX. Immobilization of ascorbic acid in the coatings was confirmed with Raman spectroscopy. The biocompatibility of the materials obtained was tested in vitro using both bone marrow- and adipose-derived mesenchymal stem cells (BMMSC and ADMSC, respectively). Proliferation rate and morphology of cells cultured in the presence of designed biomaterials were monitored after 24, 48, 120 and 168 h of propagation. The results obtained indicated that silica coatings doped with 0.4 M LAA had a positive effect on the proliferation rate of investigated cells, and in some cases on the growth pattern of culture.
authors.affiliation: Electron Microscopy Laboratory, University of Environmental and Life Sciences, Kożuchowska 5b St, 50-631 Wroclaw, Poland. Wrocławskie Centrum Badań EIT+, Stablowicka 147 St, 54-066 Wroclaw, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Marycz
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Śmieszek
authors.forename: Jakub
authors.initials: J
authors.lastname: Grzesiak
authors.forename: Anna
authors.initials: A
authors.lastname: Donesz-Sikorska
authors.forename: Justyna
authors.initials: J
authors.lastname: Krzak-Roś
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000497:Alloys; D000818:Animals; D001205:Ascorbic Acid / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000166:cytology; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D008422:Materials Testing / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D051381:Rats; D044968:Regenerative Medicine; D012822:Silicon Dioxide / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission; D013059:Spectrum Analysis, Raman; D013193:Stainless Steel / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; C426023:austenitic steel; D013193:Stainless Steel; C031462:titanium alloy (TiAl6V4); D012822:Silicon Dioxide; D014025:Titanium; D001205:Ascorbic Acid