title: Fish in chips: an automated microfluidic device to study drug dynamics in vivo using zebrafish embryos.
abstract: Interference of the Hedgehog (Hh) signaling pathway by cyclopamine leads to abnormal embryonic development. We monitor this dynamical drug effect in zebrafish embryos with highly precise microenvironment control using an integrated microfluidic device. This chip-based platform, which is programmable and automated, greatly facilitates the accuracy and reproducibility of the in vivo assays.
authors.affiliation: Biodynamic Optical Imaging Center (BIOPIC), and College of Engineering, Peking University, Beijing 100871, China. yanyi@pku.edu.cn.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Zheng
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhou
authors.forename: Xinxing
authors.initials: X
authors.lastname: Liu
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Pang
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Yanyi
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000254:growth & development; D001331:Automation; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000378:metabolism; D047108:Embryonic Development / Q000187:drug effects; D029941:Fish Proteins / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D053823:Hedgehog Proteins / Q000378:metabolism; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D015398:Signal Transduction / Q000187:drug effects; D014704:Veratrum Alkaloids / Q000737:chemistry / Q000633:toxicity*; D015027:Zebrafish / Q000254:growth & development*
chemical_list: D029941:Fish Proteins; D053823:Hedgehog Proteins; D014704:Veratrum Alkaloids; D049452:Green Fluorescent Proteins; C000541:cyclopamine