title: Modified methods for growing 3-D skin equivalents: an update.
abstract: Artificial epidermis can be reconstituted in vitro by seeding primary epidermal cells (keratinocytes) onto a supportive substrate and then growing the developing skin equivalent at the air-liquid interface. In vitro skin models are widely used to study skin biology and for industrial drug and cosmetic testing. Here, we describe updated methods for growing 3-dimensional skin equivalents using de-vitalized, de-epidermalized dermis (DED) substrates including methods for DED substrate preparation, cell seeding, growth conditions, and fixation procedures.
authors.affiliation: School of Biological and Biomedical Sciences, Durham University, Durham, UK.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Lamb
authors.forename: Carrie A
authors.initials: CA
authors.lastname: Ambler
mesh_terms: D015925:Cryopreservation; D020405:Dermis / Q000166:cytology; D000078404:Epidermal Cells; D006801:Humans; D015603:Keratinocytes / Q000166:cytology; D016612:Paraffin Embedding; D017768:Skin, Artificial; D013194:Staining and Labeling; D023822:Tissue Engineering / Q000379:methods*; D016707:Tissue Fixation