title: [Pathogenetic importance of proinflammatory cytokines in the formation and progression of fibrosis in alcoholic hepatitis].
abstract: OBJECTIVE
  To study the effect of tumor necrosis factor alpha (TNF-alpha) and interleukin 6 (IL-6) on prognosis and outcome of alcoholic hepatitis (formation of fibrosis).
  
  
  METHODS
  Overall 48 patients with alcoholic hepatitis (AH) were investigated. The control group consisted of 25 healthy donors. Morphological study of liver biopsies and 13C-methacetin breath test (13C MBT) was carried out at 15 patients with AH. Concentration of TNF-alpha, IL-6 and C-reactive protein (CRP) in blood serum was detected by immunoenzyme assay with application of commercial tests-systems Vector-Best.
  
  
  RESULTS
  At patients with AH TNF-alpha, IL-6 and CRP significantly increased in comparison to healthy persons. We found a significant correlation between fibrosis stage and TNF-alpha (r = 0.53; p < 0.01), IL-6 (r = 0.62; p < 0.01), the Knodell Histological Activity Index and TNF-alpha (r = 0.58, p < 0.05), IL-6 (r = 0.64; p < 0.01), the results of 13C MBT and TNF-alpha (r = -0.72; p < 0.01), IL-6 (r = -0.73; p < 0.01), alanine aminotransferase and TNF-alpha (r = 0.61; p < 0.01), IL-6 (r = 0.54; p < 0.01), aspartate aminotransferase and TNF-alpha (r = 0.64; p < 0.01), IL-6 (r = 0.65; p < 0.01), gamma-glutamyl transpeptidase and TNF-alpha (r = 0.46; p < 0.01), IL-6 (r = 0.48; p < 0.01).
  
  
  CONCLUSIONS
  Serum TNF-alpha and IL-6 have predictive value for development of inflammation and fibrosis in patients with AH and can be used as non-invasive markers of fibrosis.
authors.forename: I L
authors.initials: IL
authors.lastname: Kliaritskaia
authors.forename: E I
authors.initials: EI
authors.lastname: Stilidi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000410:Alanine Transaminase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000378:metabolism; D005260:Female; D006519:Hepatitis, Alcoholic / Q000097:blood* / Q000175:diagnosis; D006801:Humans; D015850:Interleukin-6 / Q000097:blood*; D008104:Liver Cirrhosis, Alcoholic / Q000097:blood* / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: D015415:Biomarkers; C508600:IL6 protein, human; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D002097:C-Reactive Protein; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase