title: Effect of DHU001, a Polyherbal Formula on Formalin-induced Paw Chronic Inflammation of Mice.
abstract: The effect of DHU001, a mixed herbal formula consisted of 7 types aqueous extracts for various respiratory disorders were evaluated on the formalin-induced paw chronic inflammation in mice after oral administration. Mice were subaponeurotically injected in the left hind paw with 0.02 ml of 3.75% formalin, then subjected to 500, 250 and 125 mg/kg of DHU001 oral administration, once a day for 10 days during which then the hind-paw thickness and volume were measured daily. The paw wet-weight, histological profiles, histomorphometrical analyses and paw tumor necrosis factor (TNF)-α contents were conducted at termination. After two formalin treatments, a marked increase in the paw thickness and volume was detected in the formalin-injected control as compared with that in the intact control, plus at the time of sacrifice the paw wet-weights, paw TNF-α contents were also dramatically increased with severe chronic inflammation signs at histopathological observations. However, these formalin-induced chronic inflammatory changes were dramatically decreased by treatment of dexamethasone and all three different dosages of DHU001. DHU001 has favorable effects on formalin-induced chronic inflammation mediated by TNF-α suppression, and DHU001 may represent an alternative approach for the treatment of chronic inflammatory diseases.
authors.affiliation: Department of Clinical Laboratory Science, College of Health and Therapy, Daegu Haany University, Gyeongsan 712-715, Korea.
authors.forename: Yoon-Hee
authors.initials: YH
authors.lastname: Cho
authors.forename: In-Kwon
authors.initials: IK
authors.lastname: Chung
authors.forename: Woo-Hyun
authors.initials: WH
authors.lastname: Cheon
authors.forename: Hyeung-Sik
authors.initials: HS
authors.lastname: Lee
authors.forename: Sae-Kwang
authors.initials: SK
authors.lastname: Ku
keywords: Chronic Inflammation; DHU001; Mouse; Polyherbal Formula; Tumor Necrosis Factor