title: Effect of oxygen tensions on the proliferation and angiogenesis of endometriosis heterograft in severe combined immunodeficiency mice.
abstract: OBJECTIVE
  To investigate the effects of oxygen on the proliferation and angiogenesis of endometriosis in vivo.
  
  
  METHODS
  Animal studies.
  
  
  METHODS
  Animal research facility.
  
  
  METHODS
  Thirty-six female severe combined immunodeficiency (SCID) mice, implanted with eutopic endometrium from seven endometriosis patients.
  
  
  METHODS
  Human eutopic endometrial tissues were randomized to normoxia, hyperoxia, or hypoxia pretreatment and were subcutaneously implanted into estrogen-treated ovariectomized SCID mice.
  
  
  METHODS
  The growth and quality of the implants were measured, and the expression of proliferation- and angiogenesis-associated markers (i.e., Ki67, CD31, vascular endothelial growth factor, and hypoxia-inducible factor-1α) were assessed using immunohistochemistry and Western blot analyses.
  
  
  RESULTS
  The growth curves of the implants were distinct with different oxygen pretreatments. The growth of the implants of the hypoxia group was significantly increased compared with the normoxia group, but the growth of the implants of the hyperoxia group was significantly decreased compared with the normoxia group. Microscopic examination indicated that lesions with hyperplastic cylindrical glandular epithelium were surrounded by the endometrial stroma in the hypoxia group, but the glandular epithelium was partially depauperate in the hyperoxia group. The expression of Ki67, CD31, vascular endothelial growth factor, and hypoxia-inducible factor-1α in the hypoxia-pretreated implants was significantly higher compared with the hyperoxia or normoxia groups.
  
  
  CONCLUSIONS
  Oxygen can alter the growth patterns of endometriosis implants in a SCID mouse model. Hypoxia pretreatment promoted the proliferation and angiogenesis of endometriosis, whereas hyperoxia pretreatment exhibited the opposite effect.
authors.affiliation: Department of Obstetrics and Gynecology, Chinese People's Liberation Army (PLA) 306 Hospital, Beijing, People's Republic of China; Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing, People's Republic of China.
authors.forename: Zechun
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing, People's Republic of China.
authors.forename: Weiyi
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing, People's Republic of China.
authors.forename: Shufang
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Department of Obstetrics and Gynecology, China-Japan Friendship Hospital, Beijing, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zou
authors.affiliation: Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing, People's Republic of China. Electronic address: Li_yali@hotmail.com.
authors.forename: Yali
authors.initials: Y
authors.lastname: Li
mesh_terms: D000328:Adult; D020533:Angiogenesis Inhibitors / Q000008:administration & dosage; D000818:Animals; D049109:Cell Proliferation; D004715:Endometriosis / Q000235:genetics / Q000473:pathology* / Q000628:therapy*; D005260:Female; D064593:Heterografts / Q000098:blood supply* / Q000473:pathology; D006801:Humans; D018496:Hyperoxia / Q000235:genetics / Q000473:pathology / Q000628:therapy; D000860:Hypoxia / Q000235:genetics / Q000473:pathology / Q000628:therapy; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000473:pathology / Q000628:therapy; D010100:Oxygen / Q000008:administration & dosage*; D011897:Random Allocation
chemical_list: D020533:Angiogenesis Inhibitors; D010100:Oxygen
keywords: Endometriosis; SCID mouse; angiogenesis; oxygen tension; proliferation