title: Hyperglycemia-induced inhibition of DJ-1 expression compromised the effectiveness of ischemic postconditioning cardioprotection in rats.
abstract: Ischemia postconditioning (IpostC) is an effective way to alleviate ischemia and reperfusion injury; however, the protective effects seem to be impaired in candidates with diabetes mellitus. To gain deep insight into this phenomenon, we explored the role of DJ-1, a novel oncogene, that may exhibit powerful antioxidant capacity in postconditioning cardioprotection in a rat model of myocardial ischemia reperfusion injury. Compared with normal group, cardiac DJ-1 was downregulated in diabetes. Larger postischemic infarct size as well as exaggeration of oxidative stress was observed, while IpostC reversed the above changes in normal but not in diabetic rats. DJ-1 was increased after ischemia and postconditioning contributed to a further elevation; however, no alteration of DJ-1 was documented in all subgroups of diabetic rats. Alteration of the cardioprotective PI3K/Akt signaling proteins may be responsible for the ineffectiveness of postconditioning in diabetes. There is a positive correlation relationship between p-Akt and DJ-1 but a negative correlation between infarct size and DJ-1, which may partially explain the interaction of DJ-1 and IpostC cardioprotection. Our result indicates a beneficial role of DJ-1 in myocardial ischemia reperfusion. Downregulation of cardiac DJ-1 may be responsible for the compromised diabetic heart responsiveness to IpostC cardioprotection.
authors.affiliation: Department of Anesthesiology, Renmin Hospital of Wuhan University, 99 Zi Yang Road, Wuhan, Hubei 430060, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Zhong-Yuan
authors.initials: ZY
authors.lastname: Xia
authors.forename: Bo
authors.initials: B
authors.lastname: Zhao
authors.forename: Shao-Qing
authors.initials: SQ
authors.lastname: Lei
authors.forename: Qing-Jun
authors.initials: QJ
authors.lastname: Yang
authors.forename: Rui
authors.initials: R
authors.lastname: Xue
authors.forename: Yan
authors.initials: Y
authors.lastname: Leng
authors.forename: Jin-Jin
authors.initials: JJ
authors.lastname: Xu
authors.forename: Zhengyuan
authors.initials: Z
authors.lastname: Xia
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D002316:Cardiotonic Agents / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000150:complications / Q000378:metabolism / Q000473:pathology; D006352:Heart Ventricles / Q000378:metabolism / Q000473:pathology; D006943:Hyperglycemia / Q000097:blood / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D057775:Ischemic Postconditioning; D016014:Linear Models; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D009203:Myocardial Infarction / Q000097:blood / Q000150:complications / Q000378:metabolism / Q000473:pathology; D010766:Phosphorylation; D000071617:Protein Deglycase DJ-1; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D001786:Blood Glucose; D002316:Cardiotonic Agents; D008869:Microtubule-Associated Proteins; D017382:Reactive Oxygen Species; D051057:Proto-Oncogene Proteins c-akt; C404267:PARK7 protein, rat; D000071617:Protein Deglycase DJ-1