title: Role of epithelial sodium channel in the realization of homeostatic effects of aldosterone on the volume of principal cells of cortical collecting ducts in rats after hypoosmotic stress.
abstract: The mechanism of aldosterone effects (10 nM) on the volume of the principal cells of cortical collecting ducts after hypoosmotic stress (280/140 mOsm/kg) was studied using fluorescence microscopy. The experiments with intracellular fluorescent dye calcein showed that aldosterone significantly decreases the amplitude and rate of increase in principal cell volume during hypoosmotic stress. Epithelial sodium channel blocker amiloride (10(-5)M) significantly attenuated the effects of aldosterone on the amplitude and rate of changes in cell volume. The obtained data attest to the contribution of epithelial sodium channel to the realization of rapid non-genomic effects of aldosterone on the amplitude and rate of changes in volume of the principal cells of cortical collecting ducts in rat kidney after hypoosmotic stress.
authors.affiliation: Institute of Cytology and Genetics, Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia. ninlo@bionet.nsc.ru.
authors.forename: N S
authors.initials: NS
authors.lastname: Logvinenko
authors.forename: E I
authors.initials: EI
authors.lastname: Solenov
authors.forename: L N
authors.initials: LN
authors.lastname: Ivanova
mesh_terms: D000450:Aldosterone / Q000494:pharmacology*; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D048429:Cell Size / Q000187:drug effects*; D062686:Epithelial Sodium Channel Blockers / Q000494:pharmacology; D053503:Epithelial Sodium Channels / Q000378:metabolism*; D005452:Fluoresceins; D005456:Fluorescent Dyes; D017136:Ion Transport / Q000187:drug effects; D007685:Kidney Tubules, Collecting / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D008856:Microscopy, Fluorescence; D009994:Osmolar Concentration; D009997:Osmotic Pressure; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000378:metabolism*; D046509:Tissue Culture Techniques
chemical_list: D062686:Epithelial Sodium Channel Blockers; D053503:Epithelial Sodium Channels; D005452:Fluoresceins; D005456:Fluorescent Dyes; D000450:Aldosterone; D000584:Amiloride; D012964:Sodium; C007740:fluorexon