title: Investigation of 5-HT3A receptor gene expression in peripheral blood mononuclear cells of individuals who had been exposed to air pollution.
abstract: The role of air pollution in exacerbation of allergic symptoms is well known. Several studies have shown the effect of air pollution on serotonergic system. The changes in serotonergic system could trigger several allergic symptoms. 5-HT(3A) is among serotonin receptors on the peripheral Blood Mononuclear Cells (PBMCs) as well as other cells. In the present study we compared the 5-HT(3A) gene expression in PBMCs of the asthmatic patients as well as individuals who had been exposed to the air pollution. Normal individuals were also included in the study as control for comparison of 5-HT(3A) gene expression. Following the synthesis of the cDNA using mRNA extracted from PBMCs the level of 5- HT(3A) gene expression was measured using real-time PCR. The results showed t a significant increase in the relative expression level of 5-HT(3A) receptor in PBMCs from asthmatic patients and individuals exposed to the air pollutants compared to normal controls. Our result indicates that significant increase in 5-HT(3A) receptor may contribute to the pathogenesis as well as allergic symptoms which resulted from air pollution.
authors.forename: Ghasem
authors.initials: G
authors.lastname: Ahangari
authors.forename: Leila Mohammadi
authors.initials: LM
authors.lastname: Amirabad
authors.forename: Sona
authors.initials: S
authors.lastname: Mozafari
authors.forename: Ali
authors.initials: A
authors.lastname: Majeidi
authors.affiliation: Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran. ghah@nigeb.ac.ir.
authors.forename: Gholamreza Derkhshan
authors.initials: GD
authors.lastname: Deilami
mesh_terms: D000328:Adult; D000393:Air Pollutants / Q000506:poisoning*; D000397:Air Pollution / Q000009:adverse effects*; D001249:Asthma / Q000097:blood / Q000139:chemically induced* / Q000235:genetics / Q000473:pathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D044406:Receptors, Serotonin, 5-HT3 / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D055815:Young Adult
chemical_list: D000393:Air Pollutants; D012333:RNA, Messenger; D044406:Receptors, Serotonin, 5-HT3