title: Comparative toxicity of low dose tributyltin chloride on serum, liver, lung and kidney following subchronic exposure.
abstract: Tributyltin (TBT) pollution is rampant worldwide and is a growing threat due to its bio-accumulative property. Isolated studies of TBT toxicity on different organs are available but consolidated information is greatly lacking. We planned this study to delineate the effect of subchronic (1 month) exposure to low dose TBT-chloride (TBTC) (1 and 5 mg/kg) in male Wistar rats. Total tin concentration was found to be significantly increased in liver, kidney and blood, and marginally in lungs. Organo-somatic indices were seen to be altered with little effect on serum biochemical markers (liver and kidney function, and general parameters). Reactive oxygen species but not lipid peroxidation content was observed to be significantly elevated both in the tissues and serum. TBTC was found to act as a hyperlipidemic agent and it also affected heme biosynthetic pathway. Hematological analysis showed that TBTC exposure resulted in minor alterations in RBC parameters. Histological studies demonstrated marked tissue damage in all the 3 organs. Calcium inhibitors (BAPTA-AM, EGTA) and antioxidants (NAC, C-PC) significantly restored TBTC induced loss in cell viability, under ex-vivo conditions. Antioxidants were evidently more efficient in comparison to the calcium inhibitors, implying major role of oxidative stress pathways in TBTC toxicity.
authors.affiliation: Immunotoxicology Division, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Lucknow 226001, India.
authors.forename: Sumonto
authors.initials: S
authors.lastname: Mitra
authors.affiliation: Immunotoxicology Division, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Lucknow 226001, India.
authors.forename: Ruchi
authors.initials: R
authors.lastname: Gera
authors.affiliation: Immunotoxicology Division, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Lucknow 226001, India.
authors.forename: Vikas
authors.initials: V
authors.lastname: Singh
authors.affiliation: Immunotoxicology Division, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Lucknow 226001, India. Electronic address: skhandelwal_itrc@rediffmail.com.
authors.forename: Shashi
authors.initials: S
authors.lastname: Khandelwal
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D014220:Trialkyltin Compounds / Q000633:toxicity*
chemical_list: D017382:Reactive Oxygen Species; D014220:Trialkyltin Compounds; C011559:tributyltin
keywords: Kidney; Liver; Lungs; Multi-organ toxicity; Subchronic exposure; Tributyltin chloride