title: First report on the occurrence of the uncultivated cluster 2 Frankia microsymbionts in soil outside the native actinorhizal host range area.
abstract: The occurrence of uncultivated Frankia was evaluated in Tunisian soils by a plant-trapping assay using Coriaria myrtifolia seedlings. Despite the lack of this compatible host plant for more than two centuries, soil-borne Frankia cells were detected in one sampled soil as shown by the development of root nodules on 2-year-old seedlings. Based on glnA sequences, Tunisian trapped Frankia strains belong to the uncultivated cluster 2 strains that associate with other Coriaria species and also with Ceanothus, Datisca and Rosaceae actinorhizal species. This is the first report on the occurrence of Frankia cluster 2 strains in soils from areas lacking compatible host plant groups.
authors.affiliation: Laboratoire Microorganismes et Biomolecules Actives, Universite de Tunis El Manar (FST) et Universite de Carthage (INSAT) 2092, Tunis, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Nouioui
authors.forename: Imed
authors.initials: I
authors.lastname: Sbissi
authors.forename: Faten
authors.initials: F
authors.lastname: Ghodhbane-Gtari
authors.forename: Kawtar Fikri
authors.initials: KF
authors.lastname: Benbrahim
authors.forename: Philippe
authors.initials: P
authors.lastname: Normand
authors.forename: Maher
authors.initials: M
authors.lastname: Gtari
mesh_terms: D019662:Cucurbitaceae / Q000382:microbiology* / Q000502:physiology; D003470:Culture Media; D004269:DNA, Bacterial / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D040161:Frankia / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D005974:Glutamate-Ammonia Ligase / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D009586:Nitrogen Fixation / Q000502:physiology; D010802:Phylogeny; D053204:Root Nodules, Plant / Q000382:microbiology* / Q000502:physiology; D013559:Symbiosis; D014416:Tunisia
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; C081173:glutamine synthetase I; D005974:Glutamate-Ammonia Ligase