title: Rugged and accurate quantitation of colchicine in human plasma to support colchicine poisoning monitoring by using turbulent-flow LC-MS/MS analysis.
abstract: BACKGROUND
  Colchicine is a common drug used in inflammatory diseases. The narrow therapeutic index requires fast and reliable techniques for its quantitation. An online, automated sample preparation using TurboFlow™ technology combined with triple-stage quadrupole MS detection was applied to identify colchicine in human plasma and follow intoxications.
  
  
  METHODS
  Plasma samples (200 µl) were mixed with deuterated colchicine and protein precipitation ZnSO4 solutions. After centrifugation, supernatants were extracted onto a Cyclone P TurboFlow column and eluted onto a narrowbore Hypersil™ GOLD column with a methanol/water gradient. Analytes were monitored in SRM mode (positive electrospray).
  
  
  RESULTS
  Total run time was 9.5 min. Calibration curves ranged from 0.342 to 17.1 ng/ml, with significant linearity (R(2) >0.99). Inter- and intra-assay precisions were <16.8% and accuracy was 84.4-110%.
  
  
  CONCLUSIONS
  This method is suitable for monitoring intoxication in patients undergoing chronic treatment and is routinely applied to toxicological samples.
authors.affiliation: Laboratoire de Toxicologie Biologique, Groupe Hospitalier St Louis Lariboisière, Assistance Publique-Hôpitaux de Paris, 2 rue Ambroise Paré, 75010 Paris, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Bourgogne
authors.forename: Marion
authors.initials: M
authors.lastname: Soichot
authors.forename: Colette
authors.initials: C
authors.lastname: Latour
authors.forename: Olivier
authors.initials: O
authors.lastname: Laprévote
mesh_terms: D002138:Calibration; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003078:Colchicine / Q000097:blood* / Q000506:poisoning; D006801:Humans; D057230:Limit of Detection; D053719:Tandem Mass Spectrometry / Q000379:methods*; D019287:Zinc Sulfate / Q000737:chemistry
chemical_list: D019287:Zinc Sulfate; D003078:Colchicine