title: Immune gene discovery in the crucian carp Carassius auratus.
abstract: The crucian carp Carassius auratus (Cyprinidae) is one of the important fish species in aquaculture. Although the crucian carp has several economic benefits, their immune system and gene information have not been investigated in depth as yet. Here, we performed the transcriptome analysis of C. auratus using the pyrosequencing method and selected several immune-related genes. Of unigenes obtained in this species, we identified a number of immune system-related genes (e.g. adhesive protein, antimicrobial protein, apoptosis- and cell cycle-related protein, cellular defense effector, immune regulator, pattern recognition protein, protease, protease inhibitor, reduction/oxidation-related protein, signal transduction-related protein and stress protein) that are potentially useful for studies on fish immunity. To be of public and practical use, we designed primer pairs of each gene from the crucian carp for real-time RT-PCR application and tested the amplicon identity of entire gene sets with the total RNA sample. For comparative analysis, we measured tissue-preferential transcript profiles of selected genes. This study will be helpful to extend our knowledge on the immune system of the crucian carp in comparative aspects and to develop the crucian carp as a potential model organism for aquatic quality monitoring in fish farming.
authors.affiliation: Research Institute for Natural Sciences, Hanyang University, Seoul 133-791, South Korea.
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Rhee
authors.affiliation: Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 133-791, South Korea.
authors.forename: Chang-Bum
authors.initials: CB
authors.lastname: Jeong
authors.affiliation: Department of Life Science, College of Convergence, Sangmyung University, Seoul 110-743, South Korea.
authors.forename: Duck-Hyun
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Division of Life Sciences, Korea Polar Research Institute, Incheon 406-840, South Korea.
authors.forename: Il-Chan
authors.initials: IC
authors.lastname: Kim
authors.affiliation: Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 133-791, South Korea.
authors.forename: Yong Sung
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Research Strategy & Planning Division, National Institute of Environmental Research, Incheon 404-708, South Korea. Electronic address: leecwoo@korea.kr.
authors.forename: Chulwoo
authors.initials: C
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Sungkyunkwan University, Suwon 440-746, South Korea. Electronic address: r_marmoratus@hanmail.net.
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics / Q000276:immunology*; D020869:Gene Expression Profiling / Q000379:methods / Q000662:veterinary*; D063990:Gene Ontology; D007107:Immune System / Q000276:immunology*; D008969:Molecular Sequence Data; D012313:RNA / Q000737:chemistry / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA
chemical_list: D012313:RNA
keywords: Carassius auratus; Crucian carp; Immune-relevant genes; Pyrosequencing