title: Breed-specific hematological phenotypes in the dog: a natural resource for the genetic dissection of hematological parameters in a mammalian species.
abstract: Remarkably little has been published on hematological phenotypes of the domestic dog, the most polymorphic species on the planet. Information on the signalment and complete blood cell count of all dogs with normal red and white blood cell parameters judged by existing reference intervals was extracted from a veterinary database. Normal hematological profiles were available for 6046 dogs, 5447 of which also had machine platelet concentrations within the reference interval. Seventy-five pure breeds plus a mixed breed control group were represented by 10 or more dogs. All measured parameters except mean corpuscular hemoglobin concentration (MCHC) varied with age. Concentrations of white blood cells (WBCs), neutrophils, monocytes, lymphocytes, eosinophils and platelets, but not red blood cell parameters, all varied with sex. Neutering status had an impact on hemoglobin concentration, mean corpuscular hemoglobin (MCH), MCHC, and concentrations of WBCs, neutrophils, monocytes, lymphocytes and platelets. Principal component analysis of hematological data revealed 37 pure breeds with distinctive phenotypes. Furthermore, all hematological parameters except MCHC showed significant differences between specific individual breeds and the mixed breed group. Twenty-nine breeds had distinctive phenotypes when assessed in this way, of which 19 had already been identified by principal component analysis. Tentative breed-specific reference intervals were generated for breeds with a distinctive phenotype identified by comparative analysis. This study represents the first large-scale analysis of hematological phenotypes in the dog and underlines the important potential of this species in the elucidation of genetic determinants of hematological traits, triangulating phenotype, breed and genetic predisposition.
authors.affiliation: Department of Clinical Sciences and Services, Regulatory T Cell Laboratory, The Royal Veterinary College, Camden Campus, Royal College Street, London, United Kingdom.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Lawrence
authors.forename: Yu-Mei Ruby
authors.initials: YM
authors.lastname: Chang
authors.forename: Balazs
authors.initials: B
authors.lastname: Szladovits
authors.forename: Lucy J
authors.initials: LJ
authors.lastname: Davison
authors.forename: Oliver A
authors.initials: OA
authors.lastname: Garden
mesh_terms: D000818:Animals; D001772:Blood Cell Count; D004285:Dogs / Q000235:genetics*; D010641:Phenotype; D025341:Principal Component Analysis; D013045:Species Specificity