title: Rapid detection for rabbit-derived dermatophytes using microsatellite-primed polymerase chain reaction.
abstract: A method exhibiting high sensitivity, specificity and rapidity to detect pathogenic dermatophytes was developed using microsatellite-primed polymerase chain reaction (PCR) in combination with a clustering method. The DNA fragments of Trichophyton mentagrophyton, Microsporum gypseum and Microsporum canis were amplified by using the primer (GACA)4 to detect the DNA polymorphism fingerprints. Twenty-one clinical strains identified as T. mentagrophyton, M. gypseum or M. canis by morphological methods were distinguished according to the differences of standard stains' bands combined with NTSYS-pc2.10 software. The results showed that there were obvious and direct differences in the bands of the three pathogenic dermatophytes, and the similarity of isolated strains and standard strains were above 90%, in line with the results of morphological identification. The method is more accurate, rapid and simple, which is meaningful for the clinical diagnosis and epidemic research of the dermatophytes.
authors.affiliation: College of Life Sciences, Taishan Medical University, Tai'an, China.
authors.forename: Zengmin
authors.initials: Z
authors.lastname: Miao
authors.forename: Song
authors.initials: S
authors.lastname: Li
authors.forename: Daijun
authors.initials: D
authors.lastname: Li
authors.forename: Chunmei
authors.initials: C
authors.lastname: Cai
authors.forename: Yumei
authors.initials: Y
authors.lastname: Cai
mesh_terms: D000818:Animals; D003883:Arthrodermataceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D018895:Microsatellite Repeats; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009172:Mycology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011817:Rabbits; D012376:Rodent Diseases / Q000175:diagnosis* / Q000382:microbiology; D013997:Time Factors; D014005:Tinea / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*
chemical_list: D017931:DNA Primers