title: Bromo- and thiomaleimides as a new class of thiol-mediated fluorescence 'turn-on' reagents.
abstract: Bromo- and thiomaleimides are shown to serve as highly effective quenchers of a covalently attached fluorophore. Reactions with thiols that lead to removal of the maleimide conjugation, or detachment of the fluorophore from the maleimide, result in 'turn-on' of the fluorescence. These reagents thus offer opportunities in thiol sensing and intracellular reporting.
authors.affiliation: Department of Chemistry, University College London, 20 Gordon St, London, UK. j.r.baker@ucl.ac.uk.
authors.forename: Judith
authors.initials: J
authors.lastname: Youziel
authors.forename: Ahmed R
authors.initials: AR
authors.lastname: Akhbar
authors.forename: Qadeer
authors.initials: Q
authors.lastname: Aziz
authors.forename: Mark E B
authors.initials: ME
authors.lastname: Smith
authors.forename: Stephen
authors.initials: S
authors.lastname: Caddick
authors.forename: Andrew
authors.initials: A
authors.lastname: Tinker
authors.forename: James R
authors.initials: JR
authors.lastname: Baker
mesh_terms: D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D008301:Maleimides / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D008301:Maleimides; D013438:Sulfhydryl Compounds