title: Effect of carotenoid β-cryptoxanthin on cellular and humoral immune response in rabbit.
abstract: Beta-cryptoxanthin (b-Cr) is a pro-vitamin A and one of the major carotenoids that can be commonly found in mammalian serum and tissues. Foods rich in certain fatty acids are known to be effective to gain a healthy immune system. In the present study, we evaluated the effect of b-Cr on rabbit humoral and cellular immune responses to have a better vision about the mechanism of effect of carotenoids on immune system. Twenty rabbits were randomly divided into five groups (4 per group): Groups consisted of: 1) control group (normal saline; 2) b-Cr (control); 3) vaccine control; 4) 5 mg/kg b-Cr o.p. + vaccine; 5) 10 mg/kg b-Cr o.p. + vaccine. Blood samples were obtained from the marginal ear artery at three time points: days 0, 14 and 21 of the study. Blood CD4+ and CD8+ lymphocytes and Serum Immunoglobulin and Cytokines content were evaluated. Results show that b-Cr administration increased the blood CD4+ lymphocytes count (P > 0.01). Serum IgG, IgM and IgA levels increased (P > 0.05) following b-Cr administration. b-Cr treatment increased serum IL-4 levels (P > 0.05). According to presented results, b-Cr may increase the humoral immunity in mammals. So, it would possible has a potentially beneficial effect on health and on prevention of the immunity related diseases.
authors.affiliation: Faculty of Medicine, Department of Biochemistry, Osmangazi University, Eskisehir, Turkey.
authors.forename: Soroush
authors.initials: S
authors.lastname: Ghodratizadeh
authors.forename: Güngör
authors.initials: G
authors.lastname: Kanbak
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Beyramzadeh
authors.forename: Zeliha Gunnur
authors.initials: ZG
authors.lastname: Dikmen
authors.forename: Salar
authors.initials: S
authors.lastname: Memarzadeh
authors.forename: Reza
authors.initials: R
authors.lastname: Habibian
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects; D065366:Cryptoxanthins; D007111:Immunity, Cellular / Q000187:drug effects*; D056724:Immunity, Humoral / Q000187:drug effects*; D007136:Immunoglobulins / Q000097:blood; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008297:Male; D009233:Myxoma virus / Q000276:immunology; D011817:Rabbits / Q000276:immunology*; D011897:Random Allocation; D014765:Viral Vaccines / Q000276:immunology; D024341:Xanthophylls / Q000494:pharmacology*
chemical_list: D065366:Cryptoxanthins; D007136:Immunoglobulins; D014765:Viral Vaccines; D024341:Xanthophylls; D015847:Interleukin-4; D007371:Interferon-gamma