title: [Role of transcription factor ETS-1 and B cell in the pathogenesis of systemic lupus erythematosus].
abstract: OBJECTIVE
  To explore the effects of transcription factor ETS-1 mRNA and B lymphocyte-associated cytokines on the differentiation of B cells in systemic lupus erythematosus (SLE) patients and explore its pathogenic and clinical significance.
  
  
  METHODS
  Thirty-one SLE patients (20 active and 11 inactive) and 15 healthy controls were enrolled. CD19+ B cells were isolated with magnetic beads. The levels of ETS-1 mRNA in B cells were determined by real-time polymerase chain reaction (PCR). Flow cytometry was used to detect the altered ratio of CD19-CD138 + plasma cells and CD19 + B cells. And enzyme-linked immunosorbent assay (ELISA) was performed to detect the serum levels of B cell differentiation-related cytokines interleukin-10 (IL-10) and APRIL.
  
  
  RESULTS
  Compared to the healthy controls, SLE patients showed decreased mRNA expression level of ETS-1 in B cells (Z = -4.218, P < 0.01) . Moreover, the expression level of ETS-1 mRNA was significantly negatively correlated with the proportion of CD19-CD138+ plasma cells (r = -0.359, P < 0.05) and negatively correlated with the CD19-CD138 +B cells/CD19+ plasma cells ratio (r = -0.493, P < 0.01) . However, there was no correlation in normal controls. Significant negative correlation existed between the expression level of ETS-1 mRNA in B cells and the serum levels of IL-10 and APRIL in active SLE patients. But no correlation existed in inactive group.
  
  
  CONCLUSIONS
  ETS-1 may participate in the pathogenesis of SLE through its effects on the differentiation of B cells and cooperation with IL-10 and APRIL.
authors.affiliation: Department of Rheumatology, Affiliated Provincial Hospital, Anhui Medical University, Hefei 230001, China.
authors.forename: Qi-jie
authors.initials: QJ
authors.lastname: Ren
authors.forename: Xiang-pei
authors.initials: XP
authors.lastname: Li
authors.forename: Jin-hui
authors.initials: JH
authors.lastname: Tao
authors.forename: Ning
authors.initials: N
authors.lastname: Yu
authors.forename: Xiao-mei
authors.initials: XM
authors.lastname: Li
authors.forename: Guo-sheng
authors.initials: GS
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Xuan
authors.initials: X
authors.lastname: Fang
mesh_terms: D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D050784:Proto-Oncogene Protein c-ets-1 / Q000378:metabolism*; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13 / Q000378:metabolism; D055815:Young Adult
chemical_list: C494041:ETS1 protein, human; C508609:IL10 protein, human; D050784:Proto-Oncogene Protein c-ets-1; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13; D016753:Interleukin-10