title: Association of five SNPs at the PARK16 locus as a susceptibility locus with Parkinson's disease for forensic application.
abstract: To investigate the association of five SNPs (rs823083, rs708723, rs4951261, rs823076 and rs16856110) at the PARK16 locus with Parkinson's disease (PD), and to potentiate its forensic application. The genomic DNAs of 215 PD patients and 212 matched controls from the northern Han Chinese population were amplified in two independent PCR systems and subsequently genotyped by digestion with the three endonucleases (Hinf I, Nco I and Msp I ). The genetic parameters and association studies were carried out with SPSS 13.0, Haploview version 4.2 and PLINK 1.07 softwares. We detected accurately all genotypes in the five SNPs with multiplex PCR-RFLP and mismatched multiplex PCR-RFLP techniques. The genotypes of four SNPs, except for rs823083, were in Hardy-Weinberg equilibrium. The four SNPs, rs16856110, rs4951261, rs708723 and rs823076, which were in linkage equilibrium, should not be associated with PD (P-values ranging from 0.077 to 0.544). The SNPs investigated at the PARK16 locus were not found to be involved in PD-associated blocks in the northern Han Chinese population. The allele distributions of rs708723, rs4951261, rs823076 and rs16856110 in the northern Han Chinese population can be highly polymorphic, which can be applied to genetic analysis and forensic practices.
authors.affiliation: School of Forensic Medicine, China Medical University, Shenyang 110001, China. cmu062208@163.com
authors.forename: Hong-Gang
authors.initials: HG
authors.lastname: Cui
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Tian
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Luo
authors.forename: Feng-Rui
authors.initials: FR
authors.lastname: Li
authors.forename: Lan-Hui
authors.initials: LH
authors.lastname: Zhu
authors.forename: Yi-Shu
authors.initials: YS
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Ren
authors.forename: Hao
authors.initials: H
authors.lastname: Pang
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000208:ethnology / Q000235:genetics; D016022:Case-Control Studies; D005260:Female; D053803:Forensic Genetics; D005787:Gene Frequency; D056726:Genetic Association Studies; D056426:Genetic Loci; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D010300:Parkinson Disease / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide