title: Artificial enzyme mimics for catalysis and double natural enzyme co-immobilization.
abstract: This work presents a new chemiluminescent (CL) probe array assay. The new type CL probe array is based on enzyme mimics of Co3O4-SiO2 mesoporous nanocomposite material, which not only have an excellent catalytic effect on the luminol-H2O2 CL reaction in an alkaline medium but also can be used for the immobilization of enzymes. The linear range of the lactose concentration is 3.0 × 10(-7) to 1.0 × 10(-5) g mL(-1) and the detection limit is 6.9 × 10(-8) g mL(-1). β-Galactosidase and glucose oxidase were selected as a model for enzyme assays to demonstrate the applicability of Co3O4-SiO2 mesoporous nanocomposite material in multienzyme immobilization. The novel bifunctional CL probe array has been successfully applied to the determination of lactose in milk.
authors.affiliation: School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
authors.forename: Zhujun
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Li
mesh_terms: D002384:Catalysis; D004800:Enzymes, Immobilized / Q000378:metabolism*; D007785:Lactose / Q000032:analysis; D008163:Luminescent Measurements / Q000379:methods*
chemical_list: D004800:Enzymes, Immobilized; D007785:Lactose