title: Evaluation of solid and porous microcarriers for cell growth and production of recombinant proteins.
abstract: Microcarrier technology opened new perspectives for anchorage-dependent cell culture, by providing increased surface areas for cell adhesion and proliferation, and therefore improving both cell and product yields obtained in these cultures. The establishment of a successful microcarrier culture depends on many factors, such as the type of microcarrier, the cells, and the culture conditions. In this chapter, the basic steps required for the evaluation and optimization of a microcarrier culture for the purpose of production of recombinant proteins are described, for both solid and porous microcarriers.
authors.affiliation: Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Braga, Portugal.
authors.forename: Maria Elisa
authors.initials: ME
authors.lastname: Rodrigues
authors.forename: Ana Rita
authors.initials: AR
authors.lastname: Costa
authors.forename: Mariana
authors.initials: M
authors.lastname: Henriques
authors.forename: Joana
authors.initials: J
authors.lastname: Azeredo
authors.forename: Rosário
authors.initials: R
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion; D002452:Cell Count; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival; D003412:Cricetulus; D016062:Porosity; D015202:Protein Engineering / Q000295:instrumentation / Q000379:methods; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins