title: Increased ZO-1 expression predicts valuable prognosis in non-small cell lung cancer.
abstract: OBJECTIVE
  The aim of this study was to investigate the expression of Zonula Occludens-1 (ZO-1) and its potential value as prognostic indicator of survival in patients with primary non-small cell lung cancer (NSCLC).
  
  
  METHODS
  Quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry with tissue microarrays were used to characterize the expression of the ZO-1 mRNA and protein in NSCLC. The correlation of ZO-1 expression with clinical characteristics and prognosis was determined by statistical analysis.
  
  
  RESULTS
  The ZO-1 mRNA and protein levels were significantly lower in NSCLC tissue compared with corresponding peritumoral tissue (P<0.05). ZO-1 protein expression in NSCLC was related to age (P=0.042) and 5-year survival (P<0.001). Kaplan-Meier survival and Cox regression analyses revealed that low ZO-1 expression (P<0.001) and later stage grouping by TNM (P=0.031) were independent factors predicting poor prognosis for patients with NSCLC.
  
  
  CONCLUSIONS
  Our findings provide the first evidence that high expression of ZO-1 is associated with good prognosis in NSCLC.
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of Nantong University Nantong, Jiangsu 226001, China.
authors.forename: Songshi
authors.initials: S
authors.lastname: Ni
authors.forename: Liqin
authors.initials: L
authors.lastname: Xu
authors.forename: Jianfei
authors.initials: J
authors.lastname: Huang
authors.forename: Jian
authors.initials: J
authors.lastname: Feng
authors.forename: Huijun
authors.initials: H
authors.lastname: Zhu
authors.forename: Gui
authors.initials: G
authors.lastname: Wang
authors.forename: Xudong
authors.initials: X
authors.lastname: Wang
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry* / Q000235:genetics / Q000401:mortality / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D046888:Tissue Array Analysis; D062826:Zonula Occludens-1 Protein / Q000032:analysis* / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein
keywords: Non-small cell lung cancer; immunohistochemistry; prognosis; zonula occludens-1