title: Enhancement of UVB radiation-mediated apoptosis by knockdown of cytosolic NADP+-dependent isocitrate dehydrogenase in HaCaT cells.
abstract: Ultraviolet B (UVB) radiation induces the production of reactive oxygen species (ROS) that promote apoptotic cell death. We showed that cytosolic NADP+-dependent isocitrate dehydrogenase (IDPc) plays an essential role in the control of cellular redox balance and defense against oxidative damage, by supplying NADPH for antioxidant systems. In this study, we demonstrated that knockdown of IDPc expression by RNA interference enhances UVB-induced apoptosis of immortalized human HaCaT keratinocytes. This effect manifested as DNA fragmentation, changes in cellular redox status, mitochondrial dysfunction, and modulation of apoptotic marker expression. Based on our findings, we suggest that attenuation of IDPc expression may protect skin from UVB-mediated damage, by inducing the apoptosis of UV-damaged cells.
authors.affiliation: School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Su Jeong
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Jeen-Woo
authors.initials: JW
authors.lastname: Park
mesh_terms: D017209:Apoptosis / Q000235:genetics / Q000528:radiation effects*; D002460:Cell Line; D003600:Cytosol / Q000201:enzymology*; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000172:deficiency* / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D014466:Ultraviolet Rays
chemical_list: D007521:Isocitrate Dehydrogenase; C039355:isocitrate dehydrogenase (NADP+)