title: Effect of IL-15 and natural killer cells on osteoclasts and osteoblasts in a mouse coculture.
abstract: This study analyzes the effect of interleukin-15 (IL-15) on osteoclast formation using a coculture of mouse osteoblasts and bone marrow cells (BMCs) stimulated with prostaglandin E2 (PGE2), which both have important role in rheumatoid arthritis (RA) and periodontal disease (PD). BMCs isolate lacking T (BM(T-)) or NK (BM(NK-)) cells, BMCs with no cells removed (BM(T+NK+)), purified NK cells, and purified T cells were each cocultured with osteoblasts in the presence or absence of PGE2 and/or IL-15. The number of both osteoclasts and osteoblasts was decreased by IL-15 in a dose-dependent manner in BM(T+NK+), BM(T-). However, the reductions were improved in BM(NK-). The expression of caspase3 in osteoblasts cocultured with NK cells was increased in a dose-dependent manner by IL-15. IL-15 stimulates apoptosis of osteoblasts via activation of NK cells. Since osteoblasts have an important role in bone formation, IL-15 may be an inflammatory bone destructive factor in RA and PD.
authors.affiliation: Department of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Suemoridori, Chikusa-ku, Nagoya, Aichi, 464-8651, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takeda
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kikuchi
authors.forename: Kyoko
authors.initials: K
authors.lastname: Soboku
authors.forename: Iichiro
authors.initials: I
authors.lastname: Okabe
authors.forename: Hiroki
authors.initials: H
authors.lastname: Mizutani
authors.forename: Akio
authors.initials: A
authors.lastname: Mitani
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ishihara
authors.forename: Toshihide
authors.initials: T
authors.lastname: Noguchi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology; D001172:Arthritis, Rheumatoid / Q000276:immunology; D001846:Bone Development / Q000276:immunology; D001854:Bone Marrow Cells / Q000276:immunology; D001842:Bone and Bones / Q000276:immunology; D053148:Caspase 3 / Q000096:biosynthesis; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D015232:Dinoprostone / Q000494:pharmacology*; D019409:Interleukin-15 / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D010010:Osteoclasts / Q000166:cytology*; D010510:Periodontal Diseases / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D019409:Interleukin-15; D053148:Caspase 3; D015232:Dinoprostone