title: Procyanidins from the stem wood of Machilus japonica and their inhibitory effect on LDL oxidation.
abstract: The stem wood of Machilus japonica Siebold & Zucc were extracted with 80 % aqueous MeOH, and the concentrated extract was successively partitioned with ethyl acetate (EtOAc), normal butanol, and water. From the EtOAc fraction, five procyanidins, procyanidin A1 (1), procyanidin A2 (2), procyanidin B7 (3), cinnamtannin B1 (4), and aesculitannin B (5), were isolated. Their chemical structures were identified through spectroscopic data analyses including NMR, MS, and IR. This is the first time any of these compounds have been isolated from this plant. The compounds were evaluated for inhibition activity on LDL oxidation. All of these compounds and the positive control, BHT, showed a very high inhibition effect with IC50 values of 0.94, 2.1, 1.8, 1.1, 1.0, and 1.9 μM, respectively.
authors.affiliation: Oriental Medicinal Materials & Processing, Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, Republic of Korea.
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Park
authors.forename: Su-Yeon
authors.initials: SY
authors.lastname: Kim
authors.forename: Na-Young
authors.initials: NY
authors.lastname: Song
authors.forename: Jin-Gyeong
authors.initials: JG
authors.lastname: Cho
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Kang
authors.forename: Tae-Sook
authors.initials: TS
authors.lastname: Jeong
authors.forename: Dae-Young
authors.initials: DY
authors.lastname: Lee
authors.forename: Geum-Soog
authors.initials: GS
authors.lastname: Kim
authors.forename: Yong-Bum
authors.initials: YB
authors.lastname: Kim
authors.forename: Hee-Cheol
authors.initials: HC
authors.lastname: Kang
authors.forename: Nam-In
authors.initials: NI
authors.lastname: Baek
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology; D019770:Lauraceae / Q000737:chemistry*; D008077:Lipoproteins, LDL / Q000737:chemistry*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry; D044945:Proanthocyanidins / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D008077:Lipoproteins, LDL; D010936:Plant Extracts; D044945:Proanthocyanidins