title: Polysaccharides from Panax japonicus C.A. Meyer and their antioxidant activities.
abstract: Polysaccharides named as PP1, PP2, PP3, PP4 and PP5 were extracted and isolated from the rhizomes of Panax japonicus C.A. Meyer, a well-known Chinese traditional medicine, by controlling the final concentration of solution to precipitate the polysaccharides. The molecular weight of polysaccharides was determined by HGPLC chromatography system. The monosaccharide composition was analyzed by Gas chromatography on an Agilent 6890A instrument using a DB-35MS column and flame-ionization detector. All of the polysaccharides were heteropolysaccharides and consisted of arabinose, glucose and galactose. The content of arabinose increased with the increasing of ethanol concentration and PP5 had the most arabinose content in these samples. IR spectra indicated obvious characteristic peaks of polysaccharide, the presence of uronic acids. Their antioxidant activities were evaluated by various established in vitro systems, including scavenging activity of superoxide anion, hydroxyl radical, ABTS and DPPH radicals. Both samples showed inhibitory effects on superoxide, hydroxyl, ABTS and DPPH radical. And PP5 shows more clearly and relatively stronger capacity than other polysaccharides on the protective effect of DNA damage.
authors.affiliation: Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, 510632 Guangzhou, China; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou 510632, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Yang
authors.forename: Rufeng
authors.initials: R
authors.lastname: Wang
authors.forename: Shaopeng
authors.initials: S
authors.lastname: Zhang
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhu
authors.forename: Jin
authors.initials: J
authors.lastname: Tang
authors.forename: Jianfang
authors.initials: J
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhang
authors.forename: Wencai
authors.initials: W
authors.lastname: Ye
authors.forename: Yonglian
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D004249:DNA Damage; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000737:chemistry; D008970:Molecular Weight; D005894:Panax / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D027343:Rhizome / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry
chemical_list: D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D011134:Polysaccharides; D013451:Sulfonic Acids; D013481:Superoxides; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant; Polysaccharides