title: Oxidative cleavage-based near-infrared fluorescent probe for hypochlorous acid detection and myeloperoxidase activity evaluation.
abstract: A near-infrared (NIR) fluorescent probe was synthesized and demonstrated to be highly selective in reaction with hypochlorous acid (HOCl), an endogenous reactive oxygen species (ROS) produced by myeloperoxidase in neutrophils. The reaction with HOCl resulted in the NIR fluorescence quenching at 774 nm and the absorbance decreasing at 710 nm, accompanied by the appearance of a new absorption band at 520 nm. The reaction mechanism was carefully examined and proposed to proceed by initial formation of chlorohydrins and subsequent degradation. This NIR fluorescent probe was successfully applied as a selective and sensitive indicator for HOCl on the basis of either colorimetry or fluorometry, which showed detection limits of 0.13 and 0.70 μM, respectively. In addition, the molecular probe was further demonstrated for NIR fluorescence imaging of HOCl in cells and for evaluating the enzymatic activity of myeloperoxidase in the HOCl generation by measuring absorbance change.
authors.affiliation: Institute of Intelligent Machines, Chinese Academy of Sciences , Hefei, Anhui 230031, People's Republic of China.
authors.forename: Mingtai
authors.initials: M
authors.lastname: Sun
authors.forename: Huan
authors.initials: H
authors.lastname: Yu
authors.forename: Houjuan
authors.initials: H
authors.lastname: Zhu
authors.forename: Fang
authors.initials: F
authors.lastname: Ma
authors.forename: Shan
authors.initials: S
authors.lastname: Zhang
authors.forename: Dejian
authors.initials: D
authors.lastname: Huang
authors.forename: Suhua
authors.initials: S
authors.lastname: Wang
mesh_terms: D045744:Cell Line, Tumor; D004789:Enzyme Activation / Q000502:physiology; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006997:Hypochlorous Acid / Q000032:analysis* / Q000378:metabolism; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000032:analysis* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D006997:Hypochlorous Acid; D009195:Peroxidase