title: Poly(A) RNA and Paip2 act as allosteric regulators of poly(A)-binding protein.
abstract: When bound to the 3' poly(A) tail of mRNA, poly(A)-binding protein (PABP) modulates mRNA translation and stability through its association with various proteins. By visualizing individual PABP molecules in real time, we found that PABP, containing four RNA recognition motifs (RRMs), adopts a conformation on poly(A) binding in which RRM1 is in proximity to RRM4. This conformational change is due to the bending of the region between RRM2 and RRM3. PABP-interacting protein 2 actively disrupts the bent structure of PABP to the extended structure, resulting in the inhibition of PABP-poly(A) binding. These results suggest that the changes in the configuration of PABP induced by interactions with various effector molecules, such as poly(A) and PABP-interacting protein 2, play pivotal roles in its function.
authors.affiliation: School of Interdisciplinary Bioscience & Bioengineering, Pohang University of Science & Technology (POSTECH), Pohang 790-784, Korea, Department of Physics, Pohang University of Science & Technology (POSTECH), Pohang 790-784, Korea, Department of Life Sciences, Pohang University of Science & Technology (POSTECH), Pohang 790-784, Korea and Division of Integrative Biosciences & Biotechnology, Pohang University of Science & Technology (POSTECH), Pohang 790-784, Korea.
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Lee
authors.forename: Jungsic
authors.initials: J
authors.lastname: Oh
authors.forename: Jonghyun
authors.initials: J
authors.lastname: Park
authors.forename: Ki Young
authors.initials: KY
authors.lastname: Paek
authors.forename: Sangchul
authors.initials: S
authors.lastname: Rho
authors.forename: Sung Key
authors.initials: SK
authors.lastname: Jang
authors.forename: Jong-Bong
authors.initials: JB
authors.lastname: Lee
mesh_terms: D000494:Allosteric Regulation; D020816:Amino Acid Motifs; D011061:Poly A / Q000378:metabolism*; D039102:Poly(A)-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism*
chemical_list: C488448:PAIP2 protein, human; D039102:Poly(A)-Binding Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; D011061:Poly A