title: Lactational exposure of phthalate impairs insulin signaling in the cardiac muscle of F1 female albino rats.
abstract: Di-2-ethylhexyl phthalate (DEHP), a ubiquitous endocrine disruptor and plasticizer of polyvinyl chloride, is being used in the manufacture of consumer and medical products as well as in children's toys. Fetuses and newborns are more sensitive to endocrine disruption. DEHP is a lipophilic substance, which could easily be transferred to the developing offspring through placenta or breast milk. DEHP alters the metabolism of the endocrine organs, which leads to energy imbalance associated with increased risk of insulin resistance, obesity and cardiovascular disease. The heart is an insulin-responsive organ. The effect of DEHP on the cardiac muscle insulin signaling remains obscure. Since the developmental period is more vulnerable to the adverse effect of DEHP, the present study was framed to study the impact of lactational exposure of DEHP on insulin signaling molecules in the cardiac muscle of F1 progeny female albino rat (postnatal day 60). Healthy dams were treated with DEHP orally (0, 1, 10 and 100 mg/kg body weight/day, respectively) from the postpartum day 1-21. Both low and high doses are relevant to the human exposure, and hence, both were used in this study. At a low dose (1 mg/kg body weight/day), obvious differences were observed in the fasting blood glucose and the insulin signaling molecule when compared to control. But marked differences were observed in the cardiac tissue insulin signaling molecules of animals treated with high doses. In conclusion, the DEHP treatment significantly increased the fasting blood glucose level and decreased the insulin receptor (IR), insulin receptor substrate (IRS-1), p-IRS-1(Tyr632), p-Akt(Ser473), plasma membrane glucose transporter (GLUT4), (14)C-2-deoxyglucose uptake and the (14)C-glucose oxidation. Conversely, Akt and GLUT4 protein in cytosol remained unaltered compared to control. Lactational exposure of DEHP impairs insulin signal transduction and glucose oxidation in the cardiac muscle of the F1 female albino rats, suggesting its possible role in the development of type 2 diabetes.
authors.affiliation: Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, 600 113, India.
authors.forename: Viswanathan
authors.initials: V
authors.lastname: Mangala Priya
authors.forename: Chinnaiyan
authors.initials: C
authors.lastname: Mayilvanan
authors.forename: Narasimhan
authors.initials: N
authors.lastname: Akilavalli
authors.forename: Parsanathan
authors.initials: P
authors.lastname: Rajesh
authors.forename: Karundevi
authors.initials: K
authors.lastname: Balasubramanian
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000139:chemically induced; D004051:Diethylhexyl Phthalate / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000633:toxicity*; D005260:Female; D051275:Glucose Transporter Type 4 / Q000378:metabolism; D007328:Insulin / Q000378:metabolism*; D055504:Insulin Receptor Substrate Proteins / Q000378:metabolism; D007774:Lactation; D009206:Myocardium / Q000378:metabolism*; D010084:Oxidation-Reduction; D010766:Phosphorylation; D010968:Plasticizers / Q000633:toxicity*; D051057:Proto-Oncogene Proteins c-akt; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D001786:Blood Glucose; D052244:Endocrine Disruptors; D051275:Glucose Transporter Type 4; D007328:Insulin; D055504:Insulin Receptor Substrate Proteins; C526296:Irs1 protein, rat; D010968:Plasticizers; C495694:Slc2a4 protein, rat; D004051:Diethylhexyl Phthalate; D051057:Proto-Oncogene Proteins c-akt