title: Epstein-Barr virus infection correlates with the expression of COX-2, p16(INK4A) and p53 in classic Hodgkin lymphoma.
abstract: Classic Hodgkin lymphoma (cHL), a germinal-center related B cell neoplasm in almost all cases, is characterized by scarcity of the neoplastic Hodgkin/Reed-Sternberg (H/RS) cells. Epstein-Barr virus (EBV) has been shown to affect cell cycle and regulation of apoptosis. In total, 95 cases of cHL were studied. Five-micrometer sections were prepared and stained with hematoxylin and eosin and immunohistochemical streptavidin-biotin methods for EBV-LMP-1, COX-2, p53, p16, ki-67 and cleaved caspase-3. In-situ hybridization for EBV encoded RNA was used to confirm the detection of EBV in H/RS. There were 49 nodular sclerosis, 32 mixed cellularity, 8 lymphocyte-rich, and 6 lymphocyte-depleted subtypes in this series of cases. EBV, COX-2, p16(INK4A) and p53 were detected in 55% (52/95), 64% (61/95), 62% (59/95), and 65% (62/95) of the cases respectively. EBV was detected in 62% (38/61), 70% (41/59), and 69% (43/62) of COX2, p16 and p53 positive cases respectively. On the other hand, EBV-non-infected cases of cHL are associated with 59% (20/34), 69% (25/36), and 73% (24/33) of COX2, p16 and p53 negative cases respectively. In conclusion, EBV infection is associated with the expression of COX-2, p16(INK4A) and p53. EBV might be the dominant factor in determining the expression of these three proteins.
authors.affiliation: Department of Pathology, Faculty of Medicine and Health Sciences, United Arab Emirates University Al Ain, United Arab Emirates.
authors.forename: Suhail
authors.initials: S
authors.lastname: Al-Salam
authors.forename: Aktham
authors.initials: A
authors.lastname: Awwad
authors.forename: Manjusha
authors.initials: M
authors.lastname: Sudhadevi
authors.forename: Sayel
authors.initials: S
authors.lastname: Daoud
authors.forename: Nico J D
authors.initials: NJ
authors.lastname: Nagelkerke
authors.forename: Antonio
authors.initials: A
authors.lastname: Castella
authors.forename: S M
authors.initials: SM
authors.lastname: Chong
authors.forename: Mouied
authors.initials: M
authors.lastname: Alashari
mesh_terms: D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000032:analysis*; D053148:Caspase 3 / Q000032:analysis; D049109:Cell Proliferation; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis*; D051546:Cyclooxygenase 2 / Q000032:analysis*; D004854:Herpesvirus 4, Human / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D006689:Hodgkin Disease / Q000378:metabolism* / Q000473:pathology / Q000821:virology*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D019394:Ki-67 Antigen / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D016539:Reed-Sternberg Cells / Q000737:chemistry* / Q000473:pathology / Q000821:virology*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*; D014763:Viral Matrix Proteins / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D019941:Cyclin-Dependent Kinase Inhibitor p16; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D019394:Ki-67 Antigen; D012367:RNA, Viral; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D014763:Viral Matrix Proteins; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; C505407:CASP3 protein, human; D053148:Caspase 3
keywords: COX-2; Epstein Barr virus; Hodgkin lymphoma; P16; P53