title: Expression of Arabidopsis thaliana xylose isomerase gene and its effect on ethanol production in Flammulina velutipes.
abstract: To improve the pentose fermentation rate in Flammulina velutipes, the putative xylose isomerase (XI) gene from Arabidopsis thaliana was cloned and introduced into F. velutipes and the gene expression was evaluated in transformants. mRNA expression of the putative XI gene and XI activity were observed in two transformants, indicating that the putative gene from A. thaliana was successfully expressed in F. velutipes as a xylose isomerase. In addition, ethanol production from xylose was increased in the recombinant strains. This is the first report demonstrating the possibility of using plant genes as candidates for improving the characteristics of F. velutipes.
authors.affiliation: Food Biotechnology Division, National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Maehara
authors.forename: Koji
authors.initials: K
authors.lastname: Takabatake
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kaneko
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000096:biosynthesis* / Q000235:genetics; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D055393:Flammulina / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D012331:RNA, Fungal / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014994:Xylose / Q000378:metabolism
chemical_list: D012331:RNA, Fungal; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000431:Ethanol; D014994:Xylose; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase
keywords: Basidiomycete fungi; Ethanol fermentation; Flammulina velutipes; Heterologous gene expression; Xylose metabolism