title: New cytotoxic spirostane saponin and biflavonoid glycoside from the leaves of Acacia saligna (Labill.) H.L. Wendl.
abstract: New naturally occurring spirostane saponin (25S)-5β-spirostan-3β-yl-3-O-β-D-xylopyranosyl(1 → 3)-O-β-D-xylopyranosyl(1 → 4)-β-D-galactopyranoside (6) and biflavonoid glycoside myricetin-3-O-rhamnoside (C7-O-C7) myricetin-3-O-rhamnoside (4) along with a series of known compounds erythrodiol (1), 3β-O-trans-p-coumaroyl-erythrodiol (2), quercetin-3-O-α-L-rhamnoside (3) and myricetin-3-O-α-L-rhamnoside (5) were separated from the leaves of Acacia saligna (Labill.), H.L. Wendl. Compounds 1 and 2 were separated for the first time from genus Acacia. The structures of compounds 1-6 were established on the basis of extensive 1D and 2D NMR spectroscopic techniques in combination with EI-MS and HR-ESI-MS. These compounds were screened for their antioxidant and cytotoxic activities against HEPG2 (liver cancer) cell line and significant results were obtained.
authors.affiliation: a Department of Pharmacognosy, Faculty of Pharmacy , Mansoura University , Mansoura 35516 , Egypt.
authors.forename: Sahar R
authors.initials: SR
authors.lastname: Gedara
authors.forename: Amal A
authors.initials: AA
authors.lastname: Galala
mesh_terms: D000045:Acacia / Q000737:chemistry*; D044946:Biflavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D004534:Egypt; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D008362:Mannosides / Q000737:chemistry / Q000302:isolation & purification; D019906:Nuclear Magnetic Resonance, Biomolecular; D018515:Plant Leaves / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013150:Spirostans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: C000591432:(25S)-5beta-spirostan-3beta-yl-3-O-beta-D-xylopyranosyl(1-3)-O--D-xylopyranosyl(1-4)-beta-D-galactopyranoside; D044946:Biflavonoids; D006027:Glycosides; D008362:Mannosides; D012503:Saponins; D013150:Spirostans; C529905:myricetin-3-O-rhamnoside; C000591433:myricetin-3-O-rhamnoside (C7-O-C7) myricetin-3-O-rhamnoside