title: Fabrication of a detection platform with boronic-acid-containing zwitterionic polymer brush.
abstract: Development of technologies for biomedical detection platform is critical to meet the global challenges of various disease diagnoses, especially for point-of-use applications. Because of its natural simplicity, effectiveness, and easy repeatability, random covalent-binding technique is widely adopted in antibody immobilization. However, its antigen-binding capacity is relatively low when compared to site-specific immobilization of antibody. Herein, we report that a detection platform modified with boronic acid (BA)-containing sulfobetaine-based polymer brush. Mainly because of the advantage of oriented immobilization of antibody endowed with BA-containing three-dimensional polymer brush architecture, the platform had a high antigen-binding capacity. Notably, nonspecific protein adsorption was also suppressed by the zwitterionic pendants, thus greatly enhanced signal-to-noise (S/N) values for antigen recognition. Furthermore, antibodies captured by BA pendants could be released in dissociation media. This new platform is promising for potential applications in immunoassays.
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.
authors.forename: Lingjie
authors.initials: L
authors.lastname: Song
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.forename: Shifang
authors.initials: S
authors.lastname: Luan
authors.forename: Jiao
authors.initials: J
authors.lastname: Ma
authors.forename: Jingchuan
authors.initials: J
authors.lastname: Liu
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Xu
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yin