title: Effects of chloroform extract of Dryopteris crassirhizoma on the ultramicroscopic structures of Meloidogyne incognita.
abstract: In our early experiments, the chloroform extract of D. crassirhizoma was demonstrated to contain the highest concentrations of total phloroglucinols among several extract fractions and possessed the most effective nematicidal activity. This study aimed to ascertain the ultrastructural changes in M. incognita after treatment with a D. crassirhizoma chloroform extract at 1 mg·mL⁻¹ for 24 h. It was found that the extract exhibited significant destructive effects on the worm's ultrastructure and caused distinctive damage to body surfaces and internal structures. These results will contribute to a deeper understanding of the nematicidal mechanism of D. crassirhizoma, as well as in the design of efficient bionematicides.
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, China ; Department of Traditional Chinese Medicine, Shanxi College of Traditional Chinese Medicine, Taiyuan 030024, China.
authors.forename: Ji-quan
authors.initials: JQ
authors.lastname: Liu
authors.forename: Shu-lian
authors.initials: SL
authors.lastname: Xie
authors.forename: Jia
authors.initials: J
authors.lastname: Feng
authors.forename: Jin
authors.initials: J
authors.lastname: Cai
mesh_terms: D000818:Animals; D000969:Antinematodal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity*; D002725:Chloroform / Q000737:chemistry; D032492:Dryopteris / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity*; D014431:Tylenchoidea / Q000187:drug effects* / Q000648:ultrastructure*
chemical_list: D000969:Antinematodal Agents; D010936:Plant Extracts; D002725:Chloroform