title: Re-scan confocal microscopy: scanning twice for better resolution.
abstract: We present a new super-resolution technique, Re-scan Confocal Microscopy (RCM), based on standard confocal microscopy extended with an optical (re-scanning) unit that projects the image directly on a CCD-camera. This new microscope has improved lateral resolution and strongly improved sensitivity while maintaining the sectioning capability of a standard confocal microscope. This simple technology is typically useful for biological applications where the combination high-resolution and high-sensitivity is required.
authors.affiliation: Van Leeuwenhoek Centre for Advanced Microscopy, Molecular Cytology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands ; These authors contributed equally to this work.
authors.forename: Giulia M R
authors.initials: GM
authors.lastname: De Luca
authors.forename: Ronald M P
authors.initials: RM
authors.lastname: Breedijk
authors.forename: Rick A J
authors.initials: RA
authors.lastname: Brandt
authors.forename: Christiaan H C
authors.initials: CH
authors.lastname: Zeelenberg
authors.forename: Babette E
authors.initials: BE
authors.lastname: de Jong
authors.forename: Wendy
authors.initials: W
authors.lastname: Timmermans
authors.forename: Leila Nahidi
authors.initials: LN
authors.lastname: Azar
authors.forename: Ron A
authors.initials: RA
authors.lastname: Hoebe
authors.forename: Sjoerd
authors.initials: S
authors.lastname: Stallinga
authors.forename: Erik M M
authors.initials: EM
authors.lastname: Manders
keywords: (110.2990) Image formation theory; (170.5810) Scanning microscopy; (170.6900) Three-dimensional microscopy; (180.1790) Confocal microscopy; (350.5730) Resolution