title: Extracellular ligninolytic enzymes production by Pleurotus eryngii on agroindustrial wastes.
abstract: Pleurotus eryngii (DC.) Gillet (MCC58) was investigated for its ligninolytic ability to produce laccase (Lac), manganese peroxidase (MnP), aryl alcohol oxidase (AAO), and lignin peroxidase (LiP) enzymes through solid-state fermentation using apricot and pomegranate agroindustrial wastes. The reducing sugar, protein, lignin, and cellulose levels in these were studied. Also, the production of these ligninolytic enzymes was researched over the growth of the microorganism throughout 20 days, and the reducing sugar, protein, and nitrogen levels were recorded during the stationary cultivation at 28 ± 0.5°C. The highest Lac activity was obtained as 1618.5 ± 25 U/L on day 12 of cultivation using apricot. The highest MnP activity was attained as 570.82 ± 15 U/L on day 17 in pomegranate culture and about the same as apricot culture. There were low LiP activities in both cultures. The maximum LiP value detected was 16.13 ± 0.8 U/L in apricot cultures. In addition, AAO activities in both cultures showed similar trends up to day 17 of cultivation, with the highest AAO activity determined as 105.99 ± 6.3 U/L on day 10 in apricot cultures. Decolorization of the azo dye methyl orange was also achieved with produced ligninolytic enzymes by P. eryngii using apricot and pomegranate wastes.
authors.affiliation: a Graduate School of Natural and Applied Sciences, Chemistry Department , Dokuz Eylül University , Buca-Izmir , Turkey.
authors.forename: Merve
authors.initials: M
authors.lastname: Akpinar
authors.forename: Raziye Ozturk
authors.initials: RO
authors.lastname: Urek
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D001391:Azo Compounds / Q000302:isolation & purification; D004396:Coloring Agents / Q000302:isolation & purification; D005285:Fermentation; D007218:Industrial Microbiology; D042845:Laccase / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism*; D029561:Lythraceae / Q000737:chemistry / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism*; D020076:Pleurotus / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D027861:Prunus / Q000737:chemistry / Q000378:metabolism; D012037:Refuse Disposal; D062611:Solid Waste / Q000032:analysis
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D062611:Solid Waste; C100258:methyl orange; D008031:Lignin; D000429:Alcohol Oxidoreductases; C077759:aryl-alcohol oxidase; D042845:Laccase; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase
keywords: Pleurotus eryngii; apricot; ligninolytic enzymes; pomegranate; solid-state fermentation