title: Isolation and characterization of a heavy metal-resistant, thermophilic esterase from a Red Sea brine pool.
abstract: The Red Sea Atlantis II brine pool is an extreme environment that displays multiple harsh conditions such as high temperature, high salinity and high concentrations of multiple, toxic heavy metals. The survival of microbes in such an environment by utilizing resistant enzymes makes them an excellent source of extremophilic enzymes. We constructed a fosmid metagenomic library using DNA isolated from the deepest and most secluded layer of this pool. We report the isolation and biochemical characterization of an unusual esterase: EstATII. EstATII is thermophilic (optimum temperature, 65°C), halotolerant (maintains its activity in up to 4.5 M NaCl) and maintains at least 60% of its activity in the presence of a wide spectrum of heavy metals. The combination of biochemical characteristics of the Red Sea Atlantis II brine pool esterase, i.e., halotolerance, thermophilicity and resistance to heavy metals, makes it a potentially useful biocatalyst.
authors.affiliation: 1] Biology Department, American University in Cairo, Cairo, Egypt [2] YJ-The Science and Technology Research Center, American University in Cairo, Cairo, Egypt.
authors.forename: Yasmine M
authors.initials: YM
authors.lastname: Mohamed
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Ghazy
authors.forename: Ahmed
authors.initials: A
authors.lastname: Sayed
authors.forename: Amged
authors.initials: A
authors.lastname: Ouf
authors.forename: Hamza
authors.initials: H
authors.lastname: El-Dorry
authors.forename: Rania
authors.initials: R
authors.lastname: Siam
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology*; D004950:Esterases / Q000302:isolation & purification* / Q000378:metabolism; D006358:Hot Temperature; D019156:Indian Ocean; D054892:Metagenome; D019216:Metals, Heavy / Q000737:chemistry; D059013:Microbial Consortia / Q000502:physiology; D008969:Molecular Sequence Data; D054712:Salinity; D012623:Seawater / Q000382:microbiology*; D016415:Sequence Alignment; D012965:Sodium Chloride
chemical_list: D019216:Metals, Heavy; D012965:Sodium Chloride; D004950:Esterases