title: Optimization and characterization of purified polysaccharide from Musa sapientum L. as a pharmaceutical excipient.
abstract: Polysaccharides from Musa sapientum (MSP) were isolated and purified by gel filtration chromatography. Elemental analysis, scanning electron microscopy (SEM), X-ray diffraction crystallography (XRD), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR) and NMR techniques were used to characterize the polysaccharide. Elemental analysis of the polysaccharide indicated that the contents of carbon, hydrogen and nitrogen to be 27.31, 5.07 and 0.62 (w/w%) respectively. SEM analysis suggested that the polysaccharide had irregular particle size, mostly seen as aggregates, and fibrous in nature. The samples had peaks at approximately 25°, 32°, 40° and 43° 2θ degrees in the XRD pattern, which indicated both crystalline and amorphous structure. The major functional groups identified from FT-IR spectrum includes 1631.38 cm⁻¹ (-COO-), 1377 cm⁻¹ (-COO-) and 3193.93 cm⁻¹ (-OH). Analytical data on MSP indicated that the major neutral sugars were α-D-glucose, β-D-glucose, rhamnose and D-glucuronic acid. The experimental work provides enough evidence to exploit this neutral biopolymer in food and pharmaceutical industry.
authors.affiliation: Girijananda Chowdhury Institute of Pharmaceutical Science, Guwahati, Assam 781017, India. Electronic address: sdash777@sify.com.
authors.forename: Dash
authors.initials: D
authors.lastname: Suvakanta
authors.forename: Murthy Padala
authors.initials: MP
authors.lastname: Narsimha
authors.forename: Deb
authors.initials: D
authors.lastname: Pulak
authors.forename: Chakraborty
authors.initials: C
authors.lastname: Joshabir
authors.forename: Das
authors.initials: D
authors.lastname: Biswajit
mesh_terms: D002152:Calorimetry, Differential Scanning; D005079:Excipients / Q000737:chemistry* / Q000302:isolation & purification; D005638:Fruit / Q000737:chemistry; D008970:Molecular Weight; D028521:Musa / Q000737:chemistry*; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D005079:Excipients; D010936:Plant Extracts; D011134:Polysaccharides
keywords: Gel filtration chromatography; Musa sapientum; Non starch polysaccharide; Pharmaceutical excipient