title: Determination of HER2 gene amplification in breast cancer using dual-color silver enhanced in situ hybridization (dc- SISH) and comparison with fluorescence ISH (FISH).
abstract: BACKGROUND
  The two basic methods that are currently accepted to identify the HER2 status are immunohistochemistry and flyorescence in situ hybridization (FISH) . The aim of this study was to perform the dual-color silver in situ hybridization (dc-SISH) technique as an alternative to FISH.
  
  
  METHODS
  A total of 40 invasive breast carcinoma cases were assessed for HER2 gene amplification by FISH and dual- color SISH.
  
  
  RESULTS
  Significant correlation was found in the HER2 expression results obtained with the two approaches (p=0.001, p<0.05). The concordance rate was 92.3%.
  
  
  CONCLUSIONS
  Foutine practical use of the dc-SISH method, which is much easier to apply, score, and evaluate, has many advantages. HER2 and CEN17 status can be evaluated simultaneously with the newly developed "Dual-Color Probe". All these specifications and the reliable results obtained support the widespread use of SISH technique in clinical practice.
authors.affiliation: Department of Pathology, Antalya Research and Training Hospital, Antalya, Turkey E-mail : betulunalmd@gmail.com.
authors.forename: Betul
authors.initials: B
authors.lastname: Unal
authors.forename: Fatma Seyda
authors.initials: FS
authors.lastname: Karaveli
authors.forename: Hadice Elif
authors.initials: HE
authors.lastname: Pestereli
authors.forename: Gulgun
authors.initials: G
authors.lastname: Erdogan
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics*; D016622:Silver Staining / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human