title: Study on the adsorption of DNA on the layered double hydroxides (LDHs).
abstract: Four kinds of layered double hydroxides (LDHs) were prepared by chemical coprecipitation method and used as DNA adsorbents. Multiple characterization tools such as power X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy and Standard electronic modules (SEM) were employed to characterize the LDHs. By examining the effect of initial concentration, solution pH, adsorption experiments were carried out to investigate the adsorption capacities of LDHs for DNA. The results revealed that the LDHs with Mg/Al=3 had higher ability on adsorbing the DNA and were not affected by pH values. The LDHs exhibited excellent adsorption properties and completely adsorbed DNA within 2h. The adsorption equilibrium data were fitted to the Langmuir and Freundlich models, showing that the Langmuir model which represented monolayer adsorption had better correlation with the adsorption linear equation. In addition, Circular dichroism (CD) spectrum, UV-vis spectorscopy and agarose gel electrophoresis revealed the integrity of DNA structure, suggesting that there had no damage on the DNA structure during the adsorption process.
authors.affiliation: College of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510006, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: College of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510006, PR China; The Key Laboratory of Environmental Protection and Eco-Remediation of Guangdong Regular Higher Education Institutions, Guangzhou 510006, PR China. Electronic address: pppxwu@scut.edu.cn.
authors.forename: Pingxiao
authors.initials: P
authors.lastname: Wu
authors.affiliation: College of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Ruan
authors.affiliation: College of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Paiyu
authors.initials: P
authors.lastname: Liu
authors.affiliation: College of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510006, PR China.
authors.forename: Nengwu
authors.initials: N
authors.lastname: Zhu
mesh_terms: D000327:Adsorption; D000818:Animals; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D024202:Electrophoretic Mobility Shift Assay; D006863:Hydrogen-Ion Concentration; D006878:Hydroxides / Q000737:chemistry*; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D012044:Regression Analysis; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013997:Time Factors; D014961:X-Ray Diffraction
chemical_list: D006878:Hydroxides; D004247:DNA
keywords: Adsorption; DNA; Layered double hydroxides; Mechanism