title: First finding of genetic and antigenic diversity in 1b-BVDV isolates from Argentina.
abstract: Infection with Bovine Viral Diarrhea Viruses (BVDV) in cattle results in a wide range of clinical manifestations, ranging from mild respiratory disease to fetal death and mucosal disease, depending on the virulence of the virus and the immune and reproductive status of the host. In this study 30 Argentinean BVDV isolates were characterized by phylogenetic analysis. The isolates were genotyped based on comparison of the 5' untranslated region (5' UTR) and the E2 gene. In both phylogenetic trees, 76% of the viruses were assigned to BVDV 1b, whereas BVDV 1a, 2a and 2b were also found. Eight of the BVDV 1b isolates were further characterized by cross-neutralization tests using guinea pig antisera and sera from bovines vaccinated with two different commercial vaccines. The results demonstrated the presence of a marked antigenic diversity among Argentinean BVDV isolates and suggest the need to incorporate BVDV 1b isolates in diagnostic strategies.
authors.affiliation: Virology Institute, Veterinary and Agricultural Science Research Centre, INTA Castelar, Buenos Aires, Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Pecora
authors.affiliation: Virology Institute, Veterinary and Agricultural Science Research Centre, INTA Castelar, Buenos Aires, Argentina. Electronic address: dmalacari@cnia.inta.gov.ar.
authors.forename: D A
authors.initials: DA
authors.lastname: Malacari
authors.affiliation: Ruminant Diseases and Immunology Research Unit USDA, Ames, USA.
authors.forename: J F
authors.initials: JF
authors.lastname: Ridpath
authors.affiliation: Virology Institute, Veterinary and Agricultural Science Research Centre, INTA Castelar, Buenos Aires, Argentina.
authors.forename: M S
authors.initials: MS
authors.lastname: Perez Aguirreburualde
authors.affiliation: Laboratorio Azul Diagnóstico, Buenos Aires, Argentina.
authors.forename: G
authors.initials: G
authors.lastname: Combessies
authors.affiliation: Laboratorio de Sanidad Animal, INTA Balcarce, Buenos Aires, Argentina.
authors.forename: A C
authors.initials: AC
authors.lastname: Odeón
authors.affiliation: Virology Institute, Veterinary and Agricultural Science Research Centre, INTA Castelar, Buenos Aires, Argentina.
authors.forename: S A
authors.initials: SA
authors.lastname: Romera
authors.affiliation: Departamento de Virología, Facultad de Farmacia y Bioquímica UBA, Buenos Aires, Argentina.
authors.forename: M D
authors.initials: MD
authors.lastname: Golemba
authors.affiliation: Virology Institute, Veterinary and Agricultural Science Research Centre, INTA Castelar, Buenos Aires, Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Wigdorovitz
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D000940:Antigenic Variation / Q000235:genetics / Q000276:immunology*; D001118:Argentina; D001483:Base Sequence; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics / Q000276:immunology*; D006168:Guinea Pigs; D008969:Molecular Sequence Data; D009500:Neutralization Tests / Q000662:veterinary; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D020121:5' Untranslated Regions; D012367:RNA, Viral; D014759:Viral Envelope Proteins
keywords: Antigenic diversity; Bovine Viral Diarrhea Virus; Cattle viruses; Pestivirus; Phylogenetic analysis