title: Heterogeneity of glycolytic oscillatory behaviour in individual yeast cells.
abstract: There are many examples of oscillations in biological systems and one of the most investigated is glycolytic oscillations in yeast. These oscillations have been studied since the 1950s in dense, synchronized populations and in cell-free extracts, but it has for long been unknown whether a high cell density is a requirement for oscillations to be induced, or if individual cells can oscillate also in isolation without synchronization. Here we present an experimental method and a detailed kinetic model for studying glycolytic oscillations in individual, isolated yeast cells and compare them to previously reported studies of single-cell oscillations. The importance of single-cell studies of this phenomenon and relevant future research questions are also discussed.
authors.affiliation: Department of Physics, University of Gothenburg, SE-41296 Gothenburg, Sweden. Electronic address: anna-karin.gustavsson@physics.gu.se.
authors.forename: Anna-Karin
authors.initials: AK
authors.lastname: Gustavsson
authors.affiliation: Department of Biochemistry, Stellenbosch University, SA-7602 Matieland, South Africa.
authors.forename: David D
authors.initials: DD
authors.lastname: van Niekerk
authors.affiliation: Department of Physics, University of Gothenburg, SE-41296 Gothenburg, Sweden.
authors.forename: Caroline B
authors.initials: CB
authors.lastname: Adiels
authors.affiliation: Department of Physics, University of Gothenburg, SE-41296 Gothenburg, Sweden.
authors.forename: Mattias
authors.initials: M
authors.lastname: Goksör
authors.affiliation: Department of Biochemistry, Stellenbosch University, SA-7602 Matieland, South Africa; Molecular Cell Physiology, Vrije Universiteit, NL-1081 Amsterdam, The Netherlands; MIB, University of Manchester, GB-M13-9PL Manchester, UK.
authors.forename: Jacky L
authors.initials: JL
authors.lastname: Snoep
mesh_terms: D006019:Glycolysis; D007700:Kinetics; D046210:Microfluidic Analytical Techniques / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D008954:Models, Biological; D009243:NAD / Q000378:metabolism*; D015003:Yeasts / Q000166:cytology / Q000378:metabolism*
chemical_list: D009243:NAD
keywords: Cell heterogeneity; Detailed kinetic model; Glycolysis; Limit-cycle oscillation; Microfluidics; Single-cell analysis