title: ICP-MS in drug development: high-throughput analysis of strontium and calcium in bone, tissue and plasma.
abstract: BACKGROUND
  The high-throughput analysis of strontium and calcium in plasma, tissue and bone (femur) using inductively coupled plasma-MS is described. Method validation, the results and experience obtained during sample analysis are highlighted.
  
  
  RESULTS
  The different matrices were destructed with concentrated nitric acid by heating at 60°C overnight. Using this approach it was possible to analyze large numbers of samples in parallel.
  
  
  CONCLUSIONS
  Inductively coupled plasma-MS proved to be a highly sensitive, robust and efficient technique for the analysis of several different biological samples. A total of 6767 samples were analyzed, and the performance of the method was illustrated by the fact that only 0.2% of the samples had to be reanalyzed due to anomalous results and ISR for all matrices fulfilled the acceptance criteria.
authors.affiliation: WIL Research Europe B.V., Hambakenwetering 7, 5231DD, 's-Hertogenbosch, The Netherlands.
authors.forename: Erik
authors.initials: E
authors.lastname: Baltussen
authors.forename: Dick
authors.initials: D
authors.lastname: Schoutsen
authors.forename: Theo
authors.initials: T
authors.lastname: Noij
authors.forename: Young H
authors.initials: YH
authors.lastname: Kim
authors.forename: Han K
authors.initials: HK
authors.lastname: Kim
authors.forename: Kwee H
authors.initials: KH
authors.lastname: Suh
mesh_terms: D000818:Animals; D002118:Calcium / Q000032:analysis* / Q000097:blood; D055808:Drug Discovery; D005260:Female; D005269:Femur / Q000737:chemistry; D057166:High-Throughput Screening Assays / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods*; D008892:Milk / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013324:Strontium / Q000032:analysis* / Q000097:blood; D014599:Uterus / Q000737:chemistry
chemical_list: D002118:Calcium; D013324:Strontium