title: A HPLC-MS/MS method for the simultaneous quantitation of six alkaloids of Rhizoma Corydalis Decumbentis in rat plasma and its application to a pharmacokinetic study.
abstract: A specific and reliable HPLC-MS/MS method was developed and validated for the simultaneous determination of six alkaloids in rat plasma, jatrorrhizine, berberine, tetrahydropalmatine, protopine, bicuculline and palmatine. The analytes were separated on a C18 column (50mm×2.1mm, 1.8μm) and a triple-quadrupole mass spectrometry equipped with an electrospray ionization (ESI) source was used for detection. The plasma sample was prepared by the simple protein precipitation and the recovery for the six analytes was over 80%. The calibration curves were linear over a concentration range of 0.38-1900.0ng/mL for jatrorrhizine, 0.57-2850.0ng/mL for berberine, 0.32-1600.0ng/mL for tetrahydropalmatine, 0.21-1050.0ng/mL for protopine, 0.34-1700.0ng/mL for bicuculline and 0.22-1100ng/mL for palmatine. The intra-day and inter-day precision was less than 15% and the relative error (RE) was all within ±15%. The validated method was successfully applied to a pharmacokinetics study in rats after oral administration of the extracts of Rhizoma Corydalis Decumbentis (a famous Chinese herb).
authors.affiliation: College of Science, Nanchang University, No. 999, Xuefu Road, Nanchang 330031, China. Electronic address: liaochuanrong@hotmail.com.
authors.forename: Chuanrong
authors.initials: C
authors.lastname: Liao
authors.affiliation: College of Pharmacy, Jilin Medical College, No. 5 Jilin Street, Jilin 132013, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Chang
authors.affiliation: Department of Pharmacology, School of Basic Medical Sciences, Shenyang Medical College, No. 146 Huanghebei Street, Huanggu District, Shenyang 110034, China.
authors.forename: Shiliang
authors.initials: S
authors.lastname: Yin
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, No. 24 Heping Road, Xiangfang District, Harbin 150040, China.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, No. 24 Heping Road, Xiangfang District, Harbin 150040, China.
authors.forename: Yonghai
authors.initials: Y
authors.lastname: Meng
mesh_terms: D000470:Alkaloids / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031311:Corydalis / Q000737:chemistry*; D016014:Linear Models; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000470:Alkaloids; D010936:Plant Extracts
keywords: Alkaloid; HPLC–MS/MS; Pharmacokinetics; Rat plasma; Rhizoma Corydalis Decumbentis