title: [Investigation of CCL1 rs159294 T/A gene polymorphism in pulmonary and extrapulmonary tuberculosis patients].
abstract: BACKGROUND
  The purpose of this study is to reveal whether CCL1 rs159294 T/A polymorphism in pulmonary and extrapulmonary tuberculosis patients pose a risk to catch tuberculosis or not.
  
  
  METHODS
  In the study, peripheral blood samples from the control group, which includes 160 patients, who consulted to Fırat University Faculty of Medicine, Pulmonology Policlinic in Elazığ province and who were diagnosed with tuberculosis; and 160 healthy individuals, were taken and put into tubes containing EDTA. Each tube contained 2 cc blood samples. DNA isolation was made from these blood samples and CCL1 rs159294 T/A polymorphism was defined with PCR-RFLP analysis.
  
  
  RESULTS
  For CCL1 rs159294 T/A polymorphism, TT genotype was found in 98 (61.3%) patients, TA genotype was found in 58 (36.3%) patients, AA genotype was found in 4 (2.5%) patients among 160 patients with tuberculosis; and TT genotype was found in 50 (70.4%) patients, TA genotype in 20 (28.2%) patients, AA genotype was found in 1 (1.4%) patient among 71 patients with pulmonary tuberculosis; TT genotype was found in 48 (53.9%) patients TA genotype was found in 38 (42.7%) patients and AA genotype was found in 3 (3.4%) patients among 89 extrapulmonary tuberculosis patients. And in control group, among 160 healthy individuals, TT genotype was found in 100 (62.5%) individuals, TA genotype was found in 58 (36.3%) individuals, AA genotype was found in 2 (1.3%) individuals and no statistically significant difference was found.
  
  
  CONCLUSIONS
  CCL1 rs159294 T/A polymorphism do not form an inclination to tuberculosis in our population.
authors.affiliation: Department of Molecular Biology and Genetic, Faculty of Science, Bartin University, Bartin, Turkey. ozdemirfethiahmet23@yahoo.com.
authors.forename: Fethi Ahmet
authors.initials: FA
authors.lastname: Özdemir
authors.forename: Deniz
authors.initials: D
authors.lastname: Erol
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Yüce
authors.forename: Vahit
authors.initials: V
authors.lastname: Konar
authors.forename: Ebru
authors.initials: E
authors.lastname: Kara Şenli
authors.forename: Funda
authors.initials: F
authors.lastname: Bulut
authors.forename: Figen
authors.initials: F
authors.lastname: Deveci
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D054402:Chemokine CCL1 / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014376:Tuberculosis / Q000235:genetics*; D014397:Tuberculosis, Pulmonary / Q000235:genetics*
chemical_list: C065971:CCL1 protein, human; D054402:Chemokine CCL1; D004247:DNA