title: More than royal food - Major royal jelly protein genes in sexuals and workers of the honeybee Apis mellifera.
abstract: BACKGROUND
  In the honeybee Apis mellifera, female larvae destined to become a queen are fed with royal jelly, a secretion of the hypopharyngeal glands of young nurse bees that rear the brood. The protein moiety of royal jelly comprises mostly major royal jelly proteins (MRJPs) of which the coding genes (mrjp1-9) have been identified on chromosome 11 in the honeybee's genome.
  
  
  RESULTS
  We determined the expression of mrjp1-9 among the honeybee worker caste (nurses, foragers) and the sexuals (queens (unmated, mated) and drones) in various body parts (head, thorax, abdomen). Specific mrjp expression was not only found in brood rearing nurse bees, but also in foragers and the sexuals.
  
  
  CONCLUSIONS
  The expression of mrjp1 to 7 is characteristic for the heads of worker bees, with an elevated expression of mrjp1-4 and 7 in nurse bees compared to foragers. Mrjp5 and 6 were higher in foragers compared to nurses suggesting functions in addition to those of brood food proteins. Furthermore, the expression of mrjp9 was high in the heads, thoraces and abdomen of almost all female bees, suggesting a function irrespective of body section. This completely different expression profile suggests mrjp9 to code for the most ancestral major royal jelly protein of the honeybee.
authors.affiliation: Institut für Biologie, Zoologie - Molekulare Ökologie, Martin-Luther-Universität Halle-Wittenberg, Hoher Weg 4, 06099 Halle, Saale, Germany. anja.buttstedt@gmail.com.
authors.forename: Anja
authors.initials: A
authors.lastname: Buttstedt
authors.forename: Robin Fa
authors.initials: RF
authors.lastname: Moritz
authors.forename: Silvio
authors.initials: S
authors.lastname: Erler