title: Molecular identification of aiiA homologous gene from endophytic Enterobacter species and in silico analysis of putative tertiary structure of AHL-lactonase.
abstract: The aiiA homologous gene known to encode AHL- lactonase enzyme which hydrolyze the N-acylhomoserine lactone (AHL) quorum sensing signaling molecules produced by Gram negative bacteria. In this study, the degradation of AHL molecules was determined by cell-free lysate of endophytic Enterobacter species. The percentage of quorum quenching was confirmed and quantified by HPLC method (p<0.0001). Amplification and sequence BLAST analysis showed the presence of aiiA homologous gene in endophytic Enterobacter asburiae VT65, Enterobacter aerogenes VT66 and Enterobacter ludwigii VT70 strains. Sequence alignment analysis revealed the presence of two zinc binding sites, "HXHXDH" motif as well as tyrosine residue at the position 194. Based on known template available at Swiss-Model, putative tertiary structure of AHL-lactonase was constructed. The result showed that novel endophytic strains of Enterobacter genera encode the novel aiiA homologous gene and its structural importance for future study.
authors.affiliation: Department of Studies in Microbiology, University of Mysore, Manasagangotri, Mysore 570006, India.
authors.forename: P S
authors.initials: PS
authors.lastname: Rajesh
authors.affiliation: Department of Studies in Microbiology, University of Mysore, Manasagangotri, Mysore 570006, India. Electronic address: raivittal@gmail.com.
authors.forename: V Ravishankar
authors.initials: VR
authors.lastname: Rai
mesh_terms: D000595:Amino Acid Sequence; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry* / Q000145:classification / Q000235:genetics*; D003198:Computer Simulation; D004754:Enterobacter / Q000201:enzymology* / Q000235:genetics*; D005798:Genes, Bacterial; D056726:Genetic Association Studies; D008666:Metalloendopeptidases / Q000737:chemistry* / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment
chemical_list: D002265:Carboxylic Ester Hydrolases; C432003:N-acyl homoserine lactonase; D008666:Metalloendopeptidases
keywords: AHL-lactonase; Enterobacter species; Quorum quenching; Tertiary structure; aiiA gene