title: Pyroptosis and adaptive immunity mechanisms are promptly engendered in mesenteric lymph-nodes during pig infections with Salmonella enterica serovar Typhimurium.
abstract: In this study, we explored the transcriptional response and the morphological changes occurring in porcine mesenteric lymph-nodes (MLN) along a time course of 1, 2 and 6 days post infection (dpi) with Salmonella Typhimurium. Additionally, we analysed the expression of some Salmonella effectors in tissue to complete our view of the processes triggered in these organs upon infection. The results indicate that besides dampening apoptosis, swine take advantage of the flagellin and prgJ expression by Salmonella Typhimuriun to induce pyroptosis in MLN, preventing bacterial dissemination. Furthermore, cross-presentation of Salmonella antigens was inferred as a mechanism that results in a rapid clearance of pathogen by cytotoxic T cells. In summary, although the Salmonella Typhimurium strain employed in this study was able to express some of its major virulence effectors in porcine MLN, a combination of early innate and adaptive immunity mechanisms might overcome virulence strategies employed by the pathogen, enabling the host to protect itself against bacterial spread beyond gut-associated lymph-nodes. Interestingly, we deduced that clathrin-mediated endocytosis could contribute to mechanisms of pathogen virulence and/or host defence in MLN of Salmonella infected swine. Taken together, our results are useful for a better understanding of the critical protective mechanisms against Salmonella that occur in porcine MLN to prevent the spread of infection beyond the intestine.
authors.forename: Rodrigo Prado
authors.initials: RP
authors.lastname: Martins
authors.forename: Carmen
authors.initials: C
authors.lastname: Aguilar
authors.forename: James E
authors.initials: JE
authors.lastname: Graham
authors.forename: Ana
authors.initials: A
authors.lastname: Carvajal
authors.forename: Rocío
authors.initials: R
authors.lastname: Bautista
authors.forename: M Gonzalo
authors.initials: MG
authors.lastname: Claros
authors.affiliation: Grupo de Genómica y Mejora Animal, Departamento de Genética, Facultad de Veterinaria, Universidad de Córdoba, Campus de Rabanales, Edificio Gregor Mendel C5, 14071, Córdoba, Spain. ge1gapaj@uco.es.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Garrido
mesh_terms: D056704:Adaptive Immunity; D000818:Animals; D017209:Apoptosis; D005786:Gene Expression Regulation; D008198:Lymph Nodes / Q000276:immunology* / Q000382:microbiology; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012481:Salmonella Infections, Animal / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D012486:Salmonella typhimurium / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity*; D013552:Swine; D013553:Swine Diseases / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D013997:Time Factors; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D037521:Virulence Factors