title: In vivo imaging of human adipose-derived stem cells in Alzheimer's disease animal model.
abstract: Stem cell therapy is a promising tool for the treatment of diverse conditions, including neurodegenerative diseases such as Alzheimer's disease (AD). To understand transplanted stem cell biology, in vivo imaging is necessary. Nanomaterial has great potential for in vivo imaging and several noninvasive methods are used, such as magnetic resonance imaging, positron emission tomography, fluorescence imaging (FI) and near-infrared FI. However, each method has limitations for in vivo imaging. To overcome these limitations, multimodal nanoprobes have been developed. In the present study, we intravenously injected human adipose-derived stem cells (hASCs) that were labeled with a multimodal nanoparticle, LEO-LIVE™-Magnoxide 675 or 797 (BITERIALS, Seoul, Korea), into Tg2576 mice, an AD mouse model. After sequential in vivo tracking using Maestro Imaging System, we found fluorescence signals up to 10 days after injection. We also found strong signals in the brains extracted from hASC-transplanted Tg2576 mice up to 12 days after injection. With these results, we suggest that in vivo imaging with this multimodal nanoparticle may provide a useful tool for stem cell tracking and understanding stem cell biology in other neurodegenerative diseases.
authors.affiliation: Gachon University of Medicine and Science, Department of Pharmacology, Incheon, Republic of Korea.
authors.forename: Sungji
authors.initials: S
authors.lastname: Ha
authors.forename: Sangzin
authors.initials: S
authors.lastname: Ahn
authors.forename: Saeromi
authors.initials: S
authors.lastname: Kim
authors.forename: Yuyoung
authors.initials: Y
authors.lastname: Joo
authors.forename: Young Hae
authors.initials: YH
authors.lastname: Chong
authors.forename: Yoo-Hun
authors.initials: YH
authors.lastname: Suh
authors.forename: Keun-A
authors.initials: KA
authors.lastname: Chang
mesh_terms: D017667:Adipocytes / Q000737:chemistry* / Q000166:cytology; D000544:Alzheimer Disease / Q000378:metabolism*; D000818:Animals; D058948:Cell Tracking / Q000379:methods*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry; D008297:Male; D051379:Mice; D061848:Optical Imaging / Q000379:methods*; D013234:Stem Cells / Q000737:chemistry* / Q000166:cytology; D051598:Whole Body Imaging
chemical_list: D005456:Fluorescent Dyes; D058185:Magnetite Nanoparticles