title: Biomimetic poly(serinol hexamethylene urea) for promotion of neurite outgrowth and guidance.
abstract: Nerve function recovery is a major technical challenge in the rehabilitation of patients suffering from severe neuropathies. Facilitating functional recovery requires the creation of a growth-permissive environment that directs the extension and myelination of surviving neurons. To this end, an electrospun nanofiber scaffold composed of arginine-glycine-aspartate-modified poly(serinol hexamethylene urea)-blend-poly-ε-caprolactone (PSHU-RGD/PCL) has been employed. Initially, we investigated the cytotoxicity of PSHU in PC12 cell culture. This was followed by functional examinations of PSHU-RGD for cell viability, proliferation, differentiation, and neurite outgrowth, and finally we examined electrospun scaffolds for guided neurite sprouting. MTT proliferation assays indicated no cytotoxic effects of polymer as compared to laminin-coated surfaces. Functional testing revealed PSHU-RGD surfaces to be comparable to the positive control, laminin-coated surface, in neurite outgrowth studies with average neurite lengths of 84.6 μm (laminin), 218.2 μm (PSHU-RGD), 570.2 μm (laminin + NGF), and 958.2 μm (PSHU-RGD + NGF) after two weeks on homogeneously modified surfaces, and 554.8 μm (nonwoven mats) and 1512.3 μm (uniaxially aligned mats) for PSHU-RGD/PCL + NGF scaffolds after one week. We created PSHU functionalized with the tripeptide, RGD, which provided chemical and physical cues to PC12 cell proliferation and differentiation. We expect that PSHU-RGD will be capable of directing and promoting neurite outgrowth in many neuropathy models.
authors.affiliation: a College of Engineering and Applied Science , University of Colorado Denver | Anschutz Medical Campus , 12700 E. 19th Avenue, Research 2 - Building P15, Aurora , CO 80045-2560 , USA.
authors.forename: Donghwa
authors.initials: D
authors.lastname: Yun
authors.forename: Amin
authors.initials: A
authors.lastname: Famili
authors.forename: Young Mook
authors.initials: YM
authors.lastname: Lee
authors.forename: Phillip M
authors.initials: PM
authors.lastname: Jenkins
authors.forename: Curt R
authors.initials: CR
authors.lastname: Freed
authors.forename: Daewon
authors.initials: D
authors.lastname: Park
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D007797:Laminin / Q000737:chemistry; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D020932:Nerve Growth Factor / Q000737:chemistry; D009416:Nerve Regeneration; D016501:Neurites / Q000502:physiology* / Q000648:ultrastructure; D009842:Oligopeptides / Q000737:chemistry; D016716:PC12 Cells; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D051381:Rats; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D007797:Laminin; D009842:Oligopeptides; D010455:Peptides; D011091:Polyesters; D011108:Polymers; C000591010:poly(serinol hexamethylene urea)-arginyl-glycyl-aspartic acid; C016240:polycaprolactone; C047981:arginyl-glycyl-aspartic acid; D020932:Nerve Growth Factor