title: Cytokine gene modification of bladder cancer cells for the establishment of bladder cancer vaccine.
abstract: A bladder tumor vaccine in mouse models was established by introducing interleukin-2 (IL-2) gene into mouse bladder cancer cells (MBT-2). Mice injected with non-modified MBT-2 cells did not reject subsequent challenge of MBT-2 cells. Mice injected with IL-2-gene-modified MBT-2 cells (MBT-2/IL-2) rejected subsequent challenge of unmodified MBT-2 cells but not 38C13 cells, which have the same genetic background but different antigenicity. According to these results, an injection of MBT-2/II.-2 can establish specific immunity against parental MBT-2 cells in mice, which is also demonstrated by cytotoxic T lymphocytes assay. Vaccinations with MBT-2/IL-2 cured 60% of bladder cancer which had been pre-established in mice. These results suggest that tumor cells genetically modified with IL-2 gene can act as a tumor vaccine which is expected to be a new method for immunotherapy of cancers.
authors.affiliation: Department of Urology, School of Medicine, Keio University, Tokyo, Japan Department of Surgery, Urology Service, Memorial Sloan-Kettering Cancer Center, New York, USA.
authors.forename: S
authors.initials: S
authors.lastname: Saito
authors.forename: H
authors.initials: H
authors.lastname: Tazaki
authors.forename: W D
authors.initials: WD
authors.lastname: Heston
authors.forename: W R
authors.initials: WR
authors.lastname: Fair
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D019496:Cancer Vaccines / Q000235:genetics* / Q000276:immunology; D002295:Carcinoma, Transitional Cell / Q000235:genetics / Q000276:immunology / Q000628:therapy*; D004195:Disease Models, Animal; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D016219:Immunotherapy, Adoptive / Q000379:methods*; D007376:Interleukin-2 / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D009368:Neoplasm Transplantation; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000276:immunology / Q000628:therapy*
chemical_list: D019496:Cancer Vaccines; C508594:IL2 protein, human; D007376:Interleukin-2
keywords: bladder cancer; gene therapy; immunotherapy; interleukin-2