title: Correlation between the frequency of Th17 cell and the expression of microRNA-206 in patients with dermatomyositis.
abstract: It was reported that IL-17 had been detected in the inflammatory infiltrates of patients with DM (dermatomyositis). In this study, we investigated the frequency of Th17 cells and the expression of microRNA-206 (miR-206) in DM patients. Firstly, we observed that the frequency of Th17 cells and the expression of transcription factors were increased significantly in the PBMCs of DM patients. Secondly, we found that there was a positive correlation between the percentages of Th17 cells and serum level of CK in DM patients. And the serum concentrations of IL-6, IL-1β, TGF-β, and IL-23, the important cytokines of Th17 differentiation, were increased in DM patients. It was predicted that Krüppel-like factor 4 (KLF4) is one of the multiple targets of miR-206. We detected the expression of miR-206 in DM patients, and it was decreased in the serum and PBMCs of DM patients. The augmented expression of KLF is accompanied by the attenuated expression of miR-206. Furthermore, a negative correlation between the percentages of Th17 cells and the expression of miR-206 in DM patients has been found. Taken together, these findings suggest the attenuated expression of miR-206, and the augmented frequency of Th17 cells in DM patients.
authors.affiliation: Department of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University School of Medical Science and Laboratory Medicine, Zhenjiang 212013, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Tang
authors.forename: Xinyu
authors.initials: X
authors.lastname: Tian
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Jie
authors.initials: J
authors.lastname: Tian
authors.forename: Ke
authors.initials: K
authors.lastname: Rui
authors.forename: Jia
authors.initials: J
authors.lastname: Tong
authors.forename: Liwei
authors.initials: L
authors.lastname: Lu
authors.forename: Huaxi
authors.initials: H
authors.lastname: Xu
authors.forename: Shengjun
authors.initials: S
authors.lastname: Wang
mesh_terms: D000328:Adult; D018791:CD4 Lymphocyte Count; D016022:Case-Control Studies; D002454:Cell Differentiation; D003402:Creatine Kinase / Q000378:metabolism; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D003882:Dermatomyositis / Q000235:genetics* / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D006836:Hydro-Lyases / Q000378:metabolism; D016130:Immunophenotyping; D000090062:Kruppel-Like Factor 4; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D058504:Th17 Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
chemical_list: D016207:Cytokines; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; C546087:MIRN206 microRNA, human; D035683:MicroRNAs; D003402:Creatine Kinase; D006836:Hydro-Lyases; C045581:lactate dehydratase