title: MicroRNA-33 regulates sterol regulatory element-binding protein 1 expression in mice.
abstract: MicroRNAs (miRs) are small non-protein-coding RNAs that bind to specific mRNAs and inhibit translation or promote mRNA degradation. Recent reports have indicated that miR-33, which is located within the intron of sterol regulatory element-binding protein (SREBP) 2, controls cholesterol homoeostasis and may be a potential therapeutic target for the treatment of atherosclerosis. Here we show that deletion of miR-33 results in marked worsening of high-fat diet-induced obesity and liver steatosis. Using miR-33(-/-)Srebf1(+/-) mice, we demonstrate that SREBP-1 is a target of miR-33 and that the mechanisms leading to obesity and liver steatosis in miR-33(-/-) mice involve enhanced expression of SREBP-1. These results elucidate a novel interaction between SREBP-1 and SREBP-2 mediated by miR-33 in vivo.
authors.affiliation: 1] Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan [2] Department of Clinical Innovative Medicine, Institute for Advancement of Clinical and Translational Science, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan [3].
authors.forename: Takahiro
authors.initials: T
authors.lastname: Horie
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Nishino
authors.forename: Osamu
authors.initials: O
authors.lastname: Baba
authors.forename: Yasuhide
authors.initials: Y
authors.lastname: Kuwabara
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Nakao
authors.forename: Masataka
authors.initials: M
authors.lastname: Nishiga
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Usami
authors.forename: Masayasu
authors.initials: M
authors.lastname: Izuhara
authors.forename: Naoya
authors.initials: N
authors.lastname: Sowa
authors.forename: Naoya
authors.initials: N
authors.lastname: Yahagi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shimano
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Matsumura
authors.forename: Kazuo
authors.initials: K
authors.lastname: Inoue
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Marusawa
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Nakamura
authors.forename: Koji
authors.initials: K
authors.lastname: Hasegawa
authors.forename: Noriaki
authors.initials: N
authors.lastname: Kume
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yokode
authors.forename: Toru
authors.initials: T
authors.lastname: Kita
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kimura
authors.forename: Koh
authors.initials: K
authors.lastname: Ono
mesh_terms: D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects; D005234:Fatty Liver / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007438:Introns; D050356:Lipid Metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009765:Obesity / Q000235:genetics* / Q000378:metabolism; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics* / Q000378:metabolism; D051782:Sterol Regulatory Element Binding Protein 2 / Q000235:genetics / Q000378:metabolism
chemical_list: D035683:MicroRNAs; C550417:Mirn33 microRNA, mouse; D051780:Sterol Regulatory Element Binding Protein 1; D051782:Sterol Regulatory Element Binding Protein 2