title: Phylogeny, ultrastructure and histopathology of Myxobolus lomi sp. nov., a parasite of Prochilodus lineatus (Valenciennes, 1836) (Characiformes: Prochilodontidae) from the Peixes River, São Paulo State, Brazil.
abstract: This paper presents the morphological, histological, molecular and ultrastructural data on Myxobolus lomi sp. nov., a parasite of the gill filaments of Prochilodus lineatus from the Peixes River (48º06'38″W; 22º 49'53.1″S), São Paulo State, Brazil. From 20 P. lineatus specimens examined, 90.0% (n=18) were infected. The plasmodia were white and round, measuring 250 to 300μm in diameter and the development occurred in the base of the gill filament. The spores showed symmetrical and smooth valves, with the polar filament having 8 to 11 coils. A thorough comparison with all the Myxobolus species described so far is provided. A partial sequencing of the 18S rDNA gene revealed approximately 1600-bp. The Myxobolus species parasite of P. lineatus did not match any of the Myxozoa available in GenBank. In the phylogenetic analysis, M. lomi sp. nov. is clustered with ten other species and only four of these parasites were from gills. Histological analysis of P. lineatus gills infected by M. lomi sp. nov. revealed numerous well-delimited cysts at the base of the primary lamella, between connective tissue and bone, next to the gill arteries. However no pronounced inflammatory response was found at the infection site.
authors.affiliation: USC - Universidade Sagrado Coração, Bauru, São Paulo, 17011-160, Brazil. Electronic address: azevedork@hotmail.com.
authors.forename: Rodney Kozlowiski
authors.initials: RK
authors.lastname: Azevedo
authors.affiliation: UNESP - Univ Estadual Paulista, Campus de Botucatu, Instituto de Biociências, Departamento de Parasitologia, Botucatu, São Paulo, 18618-970, Brazil.
authors.forename: Diego Henrique Mirandola Dias
authors.initials: DH
authors.lastname: Vieira
authors.affiliation: UNESP - Univ Estadual Paulista, Campus de Botucatu, Instituto de Biociências, Departamento de Física e Biofísica, Botucatu, São Paulo, 18618-970, Brazil.
authors.forename: Gustavo Henrique
authors.initials: GH
authors.lastname: Vieira
authors.affiliation: UNESP - Univ Estadual Paulista, Campus de Botucatu, Instituto de Biociências, Departamento de Parasitologia, Botucatu, São Paulo, 18618-970, Brazil.
authors.forename: Reinaldo José
authors.initials: RJ
authors.lastname: Silva
authors.affiliation: UFRA - Universidade Federal Rural da Amazônia, Laboratório de Pesquisa Carlos Azevedo, Belém, Pará 66077-901, Brazil.
authors.forename: Edilson
authors.initials: E
authors.lastname: Matos
authors.affiliation: USC - Universidade Sagrado Coração, Bauru, São Paulo, 17011-160, Brazil.
authors.forename: Vanessa Doro
authors.initials: VD
authors.lastname: Abdallah
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D060125:Characiformes / Q000469:parasitology*; D005393:Fish Diseases / Q000453:epidemiology / Q000469:parasitology*; D055766:Myxobolus / Q000145:classification* / Q000235:genetics / Q000648:ultrastructure*; D010273:Parasitic Diseases, Animal / Q000453:epidemiology / Q000469:parasitology; D010802:Phylogeny; D045483:Rivers
keywords: Brazil; Histopathology; Myxobolus lomi sp. nov.; Phylogeny; Prochilodus lineatus; Ultrastructure