title: Sca1-derived cells are a source of myocardial renewal in the murine adult heart.
abstract: Although the mammalian heart is one of the least regenerative organs in the body, recent evidence indicates that the myocardium undergoes a certain degree of renewal to maintain homeostasis during normal aging. However, the cellular origin of cardiomyocyte renewal has remained elusive due to lack of lineage tracing experiments focusing on putative adult cardiac precursor cells. We have generated triple-transgenic mice based on the tet-cre system to identify descendants of cells that have expressed the stem cell marker Sca1. We found a significant and lasting contribution of Sca1-derived cells to cardiomyocytes during normal aging. Ischemic damage and pressure overload resulted in increased differentiation of Sca1-derived cells to the different cell types present in the heart. Our results reveal a source of cells for cardiomyocyte renewal and provide a possible explanation for the limited contribution of Sca1-derived cells to myocardial repair under pathological conditions.
authors.affiliation: Max Planck Institute for Heart and Lung Research, Ludwigstr. 43, 61231 Bad Nauheim, Germany ; Institute of Cardiovascular Regeneration, Centre for Molecular Medicine, Goethe-University Frankfurt, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany.
authors.forename: Shizuka
authors.initials: S
authors.lastname: Uchida
authors.forename: Piera
authors.initials: P
authors.lastname: De Gaspari
authors.forename: Sawa
authors.initials: S
authors.lastname: Kostin
authors.forename: Katharina
authors.initials: K
authors.lastname: Jenniches
authors.forename: Ayse
authors.initials: A
authors.lastname: Kilic
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Izumiya
authors.forename: Ichiro
authors.initials: I
authors.lastname: Shiojima
authors.forename: Karsten
authors.initials: K
authors.lastname: Grosse Kreymborg
authors.forename: Harald
authors.initials: H
authors.lastname: Renz
authors.forename: Kenneth
authors.initials: K
authors.lastname: Walsh
authors.forename: Thomas
authors.initials: T
authors.lastname: Braun
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D006321:Heart / Q000254:growth & development*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology
chemical_list: D000950:Antigens, Ly; C074650:Ly6a protein, mouse; D008565:Membrane Proteins