title: Evaluation of intestinal permeability of vicenin-2 and lychnopholic acid from Lychnophora salicifolia (Brazilian arnicão) using Caco-2 cells.
abstract: Lychnophora salicifolia, commonly known as "arnicão", is used as an anti-inflammatory agent and as a flavoring agent in the Brazilian traditional spirit "cachaça". In this work, the permeation process of vicenin-2 (1) and lychnopholic acid (2) (major secondary metabolites from the hydroalcoholic extract) was investigated using Caco-2 cells. For this investigation, a new HPLC-DAD method was developed and validated for the quantification step. It was observed that 2 crosses the Caco-2 cell monolayer by passive diffusion. On the other hand, 1 was not transported, suggesting no absorption and no efflux of this compound in Caco-2 cells.
authors.affiliation: NPPNS (Núcleo de Pesquisa em Produtos Naturais e Sintéticos), Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo , 14040-903 Ribeirão Preto-SP, Brazil.
authors.forename: Dayana Rubio
authors.initials: DR
authors.lastname: Gouvea
authors.forename: Arthur
authors.initials: A
authors.lastname: de Barros Bello Ribeiro
authors.forename: Ursula
authors.initials: U
authors.lastname: Thormann
authors.forename: Norberto Peporine
authors.initials: NP
authors.lastname: Lopes
authors.forename: Veronika
authors.initials: V
authors.lastname: Butterweck
mesh_terms: D000042:Absorption; D047310:Apigenin; D019659:Asteraceae; D001692:Biological Transport; D001938:Brazil; D018938:Caco-2 Cells; D002463:Cell Membrane Permeability; D002851:Chromatography, High Pressure Liquid; D004058:Diffusion; D005960:Glucosides; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D015394:Molecular Structure; D010539:Permeability; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D005960:Glucosides; D012717:Sesquiterpenes; C530449:apigenin-6,8-di-C-glycopyranoside; C000589249:lychnopholic acid; D047310:Apigenin