title: The expression of type-1 and type-2 nitric oxide synthase in selected tissues of the gastrointestinal tract during mixed mycotoxicosis.
abstract: The aim of the study was to verify the hypothesis that intoxication with low doses of mycotoxins leads to changes in the mRNA expression levels of nitric oxide synthase-1 and nitric oxide synthase-2 genes in tissues of the gastrointestinal tract and the liver. The experiment involved four groups of immature gilts (with body weight of up to 25 kg) which were orally administered zearalenone in a daily dose of 40 μg/kg BW (group Z, n = 18), deoxynivalenol at 12 μg/kg BW (group D, n = 18), zearalenone and deoxynivalenol (group M, n = 18) or placebo (group C, n = 21) over a period of 42 days. The lowest mRNA expression levels of nitric oxide synthase-1 and nitric oxide synthase-2 genes were noted in the sixth week of the study, in particular in group M. Our results suggest that the presence of low mycotoxin doses in feed slows down the mRNA expression of both nitric oxide synthase isomers, which probably lowers the concentrations of nitric oxide, a common precursor of inflammation.
authors.affiliation: Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, ul. Oczapowskiego 13/29, Olsztyn 10-718, Poland. mgaja@uwm.edu.pl.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Gajęcka
authors.forename: Ewa
authors.initials: E
authors.lastname: Stopa
authors.forename: Michał
authors.initials: M
authors.lastname: Tarasiuk
authors.forename: Lukasz
authors.initials: L
authors.lastname: Zielonka
authors.forename: Maciej
authors.initials: M
authors.lastname: Gajęcki
mesh_terms: D000821:Animal Feed / Q000382:microbiology; D000818:Animals; D004305:Dose-Response Relationship, Drug; D041981:Gastrointestinal Tract / Q000378:metabolism* / Q000382:microbiology; D005786:Gene Expression Regulation; D008099:Liver / Q000378:metabolism / Q000382:microbiology; D015651:Mycotoxicosis / Q000378:metabolism* / Q000473:pathology; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013552:Swine; D014255:Trichothecenes / Q000008:administration & dosage; D015025:Zearalenone / Q000008:administration & dosage
chemical_list: D012333:RNA, Messenger; D014255:Trichothecenes; D009569:Nitric Oxide; D015025:Zearalenone; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; C007262:deoxynivalenol