title: α2,6 sialylation associated with increased beta 1,6-branched N-oligosaccharides influences cellular adhesion and invasion.
abstract: Expression of β1,6-branched N-linked oligosaccharides have a definite association with invasion and metastasis of cancer cells. However, the mechanism by which these oligosaccharides regulate these processes is not well understood. Invasive variants of B16 murine melanoma, B16F10 (parent) and B16BL6 (highly invasive variant) cell lines have been used for these studies. We demonstrate that substitution of α2,6-linked sialic acids on multiantennary structures formed as a result of β1,6-branching modulate cellular adhesion on both extracellular matrix (ECM) and basement membrane (BM) components. Removal of α2,6 sialic acids either by enzymatic desialylation or by stably down-regulating the ST6Gal-I (enzyme that catalyses the addition of α2,6-linked sialic acids on N-linked oligosaccharides) by lentiviral driven shRNA decreased the adhesion on both ECM and BM components and invasion through reconstituted BM matrigel.
authors.affiliation: Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre (TMC), Sector 22, Kharghar, Navi Mumbai 410 210, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Ranjan
authors.forename: Rajiv D
authors.initials: RD
authors.lastname: Kalraiya
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D005353:Fibronectins / Q000378:metabolism; D055785:Gene Knockdown Techniques; D006031:Glycosylation; D008546:Melanoma, Experimental; D051379:Mice; D009361:Neoplasm Invasiveness; D025942:Oligosaccharides, Branched-Chain / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D034741:RNA, Small Interfering / Q000235:genetics; D012794:Sialic Acids / Q000378:metabolism*; D012799:Sialyltransferases / Q000235:genetics / Q000378:metabolism; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
chemical_list: D005353:Fibronectins; D025942:Oligosaccharides, Branched-Chain; D034741:RNA, Small Interfering; D012794:Sialic Acids; D012799:Sialyltransferases; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase