title: Interaction of polyacrylic acid coated and non-coated iron oxide nanoparticles with human neutrophils.
abstract: Iron oxide nanoparticles (ION), with different coatings and sizes, have attracted extensive interest in the last years to be applied in drug delivery, cancer therapy and as contrast agents in imagiologic techniques such as magnetic resonance imaging. However, the safety of these nanoparticles is still not completely established, particularly to host defense systems that are usually recruited for their clearance from the body. In this paper, given the importance of neutrophils in the immune response of the organism to nanoparticles, the effect of polyacrylic acid (PAA)-coated and non-coated ION on human neutrophils was evaluated in vitro, namely their capacity to activate the oxidative burst and to modify their lifespan. The obtained results showed that the studied PAA-coated and non-coated ION triggered neutrophils' oxidative burst in a NADPH oxidase dependent manner, and that PAA-coated ION increased - while non-coated ION prevented - apoptotic signaling and apoptosis. These effects may have important clinical implications in biomedical applications of ION.
authors.affiliation: REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal.
authors.forename: Diana
authors.initials: D
authors.lastname: Couto
authors.affiliation: REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal.
authors.forename: Marisa
authors.initials: M
authors.lastname: Freitas
authors.affiliation: REQUIMTE, Laboratory of Toxicology, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal.
authors.forename: Vânia
authors.initials: V
authors.lastname: Vilas-Boas
authors.affiliation: REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal.
authors.forename: Irene
authors.initials: I
authors.lastname: Dias
authors.affiliation: Service of Clinical Hematology, Santo António Hospital, Porto, Portugal.
authors.forename: Graça
authors.initials: G
authors.lastname: Porto
authors.affiliation: Laboratory of Nanotechnology and Magnetism, Institute of Technological Research, IIT, University of Santiago de Compostela (USC), Santiago de Compostela, Spain.
authors.forename: M Arturo
authors.initials: MA
authors.lastname: Lopez-Quintela
authors.affiliation: International Iberian Nanotechnology Laboratory, Braga, Portugal.
authors.forename: José
authors.initials: J
authors.lastname: Rivas
authors.affiliation: International Iberian Nanotechnology Laboratory, Braga, Portugal.
authors.forename: Paulo
authors.initials: P
authors.lastname: Freitas
authors.affiliation: REQUIMTE, Laboratory of Toxicology, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal.
authors.forename: Félix
authors.initials: F
authors.lastname: Carvalho
authors.affiliation: REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal. Electronic address: egracas@ff.up.pt.
authors.forename: Eduarda
authors.initials: E
authors.lastname: Fernandes
mesh_terms: D000180:Acrylic Resins / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D020169:Caspases / Q000378:metabolism; D020099:Coated Materials, Biocompatible / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005290:Ferric Compounds / Q000633:toxicity*; D005296:Ferrous Compounds / Q000633:toxicity*; D006801:Humans; D053768:Metal Nanoparticles / Q000633:toxicity*; D019255:NADPH Oxidases / Q000378:metabolism; D009336:Necrosis; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D016897:Respiratory Burst / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D000180:Acrylic Resins; D020099:Coated Materials, Biocompatible; D005290:Ferric Compounds; D005296:Ferrous Compounds; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C000499:ferric oxide; C006903:carbopol 940; D019255:NADPH Oxidases; D020169:Caspases; C034236:ferrous oxide
keywords: Apoptosis; Human neutrophils; Iron oxide nanoparticles; Nanotoxicology; Oxidative burst; Polyacrylic acid coating