title: Biohydrogen production from sugarcane bagasse by integrating dark- and photo-fermentation.
abstract: Hydrogen production from sugarcane bagasse (SCB) by integrating dark-fermentation by Enterobacter aerogenes MTCC 2822 and photo-fermentation by Rhodopseudomonas BHU 01 was investigated. The SCB was hydrolysed by sulphuric acid and the hydrolysate detoxified by passing through adsorbent resin column (Amberlite XAD-4) to remove the inhibitory furfural, and subjected to dark-fermentation. The cellulosic residue from acid hydrolysis was hydrolysed by the new isolate Cellulomonas fimi to release sugars for H2 production by E. aerogenes, through simultaneous saccharification, filtration and fermentation (SSFF). Cumulative H2 production during dark-fermentation and SSFF was 1000 and 613 ml/L, respectively. The spent media of dark-fermentation and SSFF were utilized for photo-fermentation by Rhodopseudomonas BHU 01. The cumulative H2 production was 755 ml/L for dark-fermentation and 351 ml/L for SSFF spent medium.
authors.affiliation: Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Pankaj K
authors.initials: PK
authors.lastname: Rai
authors.affiliation: Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India. Electronic address: spsinghbhu@rediffmail.com.
authors.forename: S P
authors.initials: SP
authors.lastname: Singh
authors.affiliation: Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India.
authors.forename: R K
authors.initials: RK
authors.lastname: Asthana
authors.affiliation: Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Shweta
authors.initials: S
authors.lastname: Singh
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D056804:Biofuels / Q000382:microbiology; D050260:Carbohydrate Metabolism / Q000187:drug effects; D040143:Cellulomonas / Q000187:drug effects / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D003624:Darkness; D005285:Fermentation / Q000187:drug effects; D005374:Filtration; D005662:Furaldehyde / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D006868:Hydrolysis / Q000187:drug effects; D031786:Saccharum / Q000187:drug effects / Q000378:metabolism*; D013464:Sulfuric Acids / Q000494:pharmacology; D013997:Time Factors
chemical_list: D056804:Biofuels; D013464:Sulfuric Acids; D006859:Hydrogen; D002482:Cellulose; C027433:bagasse; D005662:Furaldehyde; C033158:sulfuric acid; D019342:Acetic Acid
keywords: Acid hydrolysis; H(2) Production; SSFF; Sugarcane bagasse