title: Design of an environmentally sensitive fluorescent 8-aza-7-deaza-2'-deoxyadenosine derivative with dual fluorescence for the specific detection of thymine.
abstract: A novel environmentally sensitive fluorescent (ESF) purine nucleoside, (cna)A, was synthesized and its photophysical properties were investigated. The base-modified fluorescent nucleoside (cna)A exhibited remarkable solvatochromicity and environmentally sensitive dual fluorescence. By using (cna)A, we have developed highly thymine (T) selective fluorescent DNA probes that can sense the corresponding T in a target DNA/RNA sequence, as measured by a distinct change in emission wavelength.
authors.affiliation: Department of Chemical Biology and Applied Chemistry, College of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan. saitoy@chem.ce.nihon-u.ac.jp.
authors.forename: Azusa
authors.initials: A
authors.lastname: Suzuki
authors.forename: Nobukatsu
authors.initials: N
authors.lastname: Nemoto
authors.forename: Isao
authors.initials: I
authors.lastname: Saito
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Saito
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013941:Thymine / Q000032:analysis*
chemical_list: C000592244:4-amino-3-(2-(6-cyano-2-naphthyl)ethynyl)-1-(2-deoxypentofuranosyl)-1H-pyrazolo(3,4-d)pyrimidine; D005456:Fluorescent Dyes; D000241:Adenosine; D013941:Thymine