title: Estrogenic Activity of Persistent Organic Pollutants and Parabens Based on the Stably Transfected Human Estrogen Receptor-α Transcriptional Activation Assay (OECD TG 455).
abstract: Screening of estrogenic activity on dichloro diphenyl trichloroethane (DDT), dichloro diphenyl dichloro ethylene (DDE), dieldrin, heptachlor, aldrin, chlordane, lindane, polybrominated diphenyl ethers (PBDE) and parabens was compared using Organization for Economic Cooperation and Development (OECD) test guideline 455 (TG455). The estrogenic activity of DDT was 58,000-fold (PC50, 1.67 × 10(-6) M) less than 17β-estradiol(E2) (PC50, 2.88 × 10(-11) M) but DDE, dieldrin, heptachlor, aldrin, chlordane, lindane and PBDE did not show any estrogenic activity in this assay system. In the case of paraben compounds, the rank of relative transcriptional activation (logRTA) was butyl paraben -1.63752 (PC50, 1.25 × 10(-7)M) > isobutyl paraben -2.34008 (PC50, 6.3 × 10(-7)M) > ethyl paraben -2.64016 (PC50, 1.26 × 10(-6) M) > isopropyl paraben -2.73993 (PC50, 1.58 × 10(-6)M) > propyl paraben -2.84164 (PC50, 2.0 × 10(-6) M). Our data suggest that OECD test guideline TG455 may be useful as a screening tool for potential endocrine disruptors.
authors.affiliation: Health Effects Analysis Team, National Institute of Food and Drug Safety Evaluation, 643 Yeonje-ri, Gangoe-myeon, Cheongwon-gun, Chungbuk 363-951, Korea.
authors.forename: Tae Sung
authors.initials: TS
authors.lastname: Kim
authors.forename: Chang Yeong
authors.initials: CY
authors.lastname: Kim
authors.forename: Hae Kyung
authors.initials: HK
authors.lastname: Lee
authors.forename: Il Hyun
authors.initials: IH
authors.lastname: Kang
authors.forename: Mi Gyeong
authors.initials: MG
authors.lastname: Kim
authors.forename: Ki Kyung
authors.initials: KK
authors.lastname: Jung
authors.forename: Yong Kwan
authors.initials: YK
authors.lastname: Kwon
authors.forename: Hye-Seon
authors.initials: HS
authors.lastname: Nam
authors.forename: Soon Keun
authors.initials: SK
authors.lastname: Hong
authors.forename: Hyung Sik
authors.initials: HS
authors.lastname: Kim
authors.forename: Hae Jung
authors.initials: HJ
authors.lastname: Yoon
authors.forename: Gyu Seek
authors.initials: GS
authors.lastname: Rhee
keywords: DDT; Endocrine disruptors; Estrogenic activity; OECD test guideline 455; Parabens