title: Two methods to simulate intrapulpal pressure: effects upon bonding performance of self-etch adhesives.
abstract: OBJECTIVE
  To evaluate the effects of two methods to simulate physiological pulpal pressure on the dentine bonding performance of two all-in-one adhesives and a two-step self-etch silorane-based adhesive by means of microtensile bond strength (μTBS) and nanoleakage surveys.
  
  
  METHODS
  The self-etch adhesives [G-Bond Plus (GB), Adper Easy Bond (EB) and silorane adhesive (SIL)] were applied to flat deep dentine surfaces from extracted human molars. The restorations were constructed using resin composites Filtek Silorane or Filtek Z350 (3M ESPE). After 24 h using the two methods of simulated pulpal pressure or no pulpal pressure (control groups), the bonded teeth were cut into specimens and submitted to μTBS and silver uptake examination. Results were analysed with two-way anova and Tukey's test (P < 0.05).
  
  
  RESULTS
  Both methods of simulated pulpal pressure led statistically similar μTBS for all adhesives. No difference between control and pulpal pressure groups was found for SIL and GB. EB led significant drop (P = 0.002) in bond strength under pulpal pressure. Silver impregnation was increased after both methods of simulated pulpal pressure for all adhesives, and it was similar between the simulated pulpal pressure methods.
  
  
  CONCLUSIONS
  The innovative method to simulate pulpal pressure behaved similarly to the classic one and could be used as an alternative. The HEMA-free one-step and the two-step self-etch adhesives had acceptable resistance against pulpal pressure, unlike the HEMA-rich adhesive.
authors.affiliation: Division of Dental Materials, Department of Restorative Dentistry, Piracicaba Dental School, State University of Campinas, Piracicaba, Brazil.
authors.forename: V P
authors.initials: VP
authors.lastname: Feitosa
authors.forename: V B
authors.initials: VB
authors.lastname: Gotti
authors.forename: C V
authors.initials: CV
authors.lastname: Grohmann
authors.forename: G
authors.initials: G
authors.lastname: Abuná
authors.forename: L
authors.initials: L
authors.lastname: Correr-Sobrinho
authors.forename: M A C
authors.initials: MA
authors.lastname: Sinhoreti
authors.forename: A B
authors.initials: AB
authors.lastname: Correr
mesh_terms: D000134:Acid Etching, Dental; D000269:Adhesives; D000293:Adolescent; D000328:Adult; D001840:Dental Bonding; D003738:Dental Cements; D003782:Dental Pulp / Q000502:physiology*; D006801:Humans; D055815:Young Adult
chemical_list: D000269:Adhesives; D003738:Dental Cements
keywords: dentin adhesion; microtensile bond strength; nanoleakage; pulpal pressure; silorane