title: New approach of bone marrow-derived mesenchymal stem cells and human amniotic epithelial cells applications in accelerating wound healing of irradiated albino rats.
abstract: OBJECTIVE
  Irradiated wound healing is a highly complex and dynamic process. The latest technology making a huge difference in this process is stem cell therapy. The goal of this study was to evaluate the use of bone marrow-derived mesenchymal stem cells (BM-MSCs) or human amniotic epithelial cells (HAECs) in the healing of irradiated wounds.
  
  
  RESULTS
  Forty five male albino rats were subjected to whole body 6 gray gamma radiations. One day post irradiation, full-thickness incisional wound was created in the tibial skin. The rats were randomly equally divided into three groups. The incisions of the first group (gp I) were injected intra-dermally with saline before stitching and those of both the second (gp II) and the third groups (gp III) were intradermally injected with BM-MSCs and HAECs before stitching respectively. Animals were sacrificed after the third, seventh and fourteenth days postoperative. The healing process was assessed histopathologically. CXCL-5, SDF-1 and Transforming growth factor-beta 1 (TGF-β1) expression were also detected in biopsies from all wounds. Expression of TGF-β1 in gp I was more than the other groups leading to severe inflammation, deficient healed dermis and delayed reepithelialization. SDF-1 expression was high in gp II while CXCL-5 expression was high in gp III causing accelerated wound healing. BM-MSCs showed a great effect on the quality of the dermis, while superiority of the epithelium and its appendages were achieved in HAECs group.
  
  
  CONCLUSIONS
  Using BM-MSCs and HAECs could be used safely in case of irradiated wounds.
authors.affiliation: Department of Oral Biology, Faculty of Oral and Dental Medicine, Cairo University.
authors.forename: Samah S
authors.initials: SS
authors.lastname: Mehanni
authors.forename: Noha F
authors.initials: NF
authors.lastname: Ibrahim
authors.forename: Alyaa R
authors.initials: AR
authors.lastname: Hassan
authors.forename: Laila A
authors.initials: LA
authors.lastname: Rashed
keywords: BM-MSCs; HAECs; Wound healing