title: Study on interaction between palladium(ІІ)-Linezolid chelate with eosin by resonance Rayleigh scattering, second order of scattering and frequency doubling scattering methods using Taguchi orthogonal array design.
abstract: Linezolid reacted with palladium to form 1:1 binary cationic chelate which further reacted with eosin dye to form 1:1 ternary ion association complex at pH 4 of Walpole's acetate buffer in the presence of methyl cellulose. As a result not only absorption spectra were changed but Resonance Rayleigh Scattering (RRS), Second-order Scattering (SOS) and Frequency Doubling Scattering (FDS) intensities were greatly enhanced. The analytical wavelengths of RRS, SOS and FDS (λex/λem) of ternary complex were located at 538 nm/538nm, 240 nm/480 nm and 660 nm/330 nm, respectively. The linearity range for RRS, SOS and FDS methods were 0.01-0.5 μg mL(-1), 0.1-2 μg mL(-1) and 0.2-1.8 μg mL(-1), respectively. The sensitivity order of three methods was as RRS>SOS>FDS. Accuracy of all methods were determined by recovery studies and showed recovery between 98% and 102%. Intraday and inter day precision were checked for all methods and %RSD was found to be less than 2 for all methods. The effects of foreign substances were tested on RRS method and it showed the method had good selectivity. For optimization of process parameter, Taguchi orthogonal array design L8(2(4)) was used and ANOVA was adopted to determine the statistically significant control factors that affect the scattering intensities of methods. The reaction mechanism, composition of ternary ion association complex and reasons for scattering intensity enhancement was discussed in this work.
authors.affiliation: Quality Assurance Laboratory, Centre of Relevance and Excellence in Novel Drug Delivery System, Pharmacy Department, The Maharaja Sayajirao University of Baroda, G.H. Patel Building, Donor's Plaza, Fatehgunj, Vadodara 390 002, Gujarat, India. Electronic address: dishathakkar282@gmail.com.
authors.forename: Disha
authors.initials: D
authors.lastname: Thakkar
authors.affiliation: Quality Assurance Laboratory, Centre of Relevance and Excellence in Novel Drug Delivery System, Pharmacy Department, The Maharaja Sayajirao University of Baroda, G.H. Patel Building, Donor's Plaza, Fatehgunj, Vadodara 390 002, Gujarat, India.
authors.forename: Bhavesh
authors.initials: B
authors.lastname: Gevriya
authors.affiliation: Quality Assurance Laboratory, Centre of Relevance and Excellence in Novel Drug Delivery System, Pharmacy Department, The Maharaja Sayajirao University of Baroda, G.H. Patel Building, Donor's Plaza, Fatehgunj, Vadodara 390 002, Gujarat, India. Electronic address: rajshreemashru@yahoo.com.
authors.forename: R C
authors.initials: RC
authors.lastname: Mashru
mesh_terms: D000081:Acetamides / Q000097:blood / Q000737:chemistry*; D000704:Analysis of Variance; D002614:Chelating Agents / Q000737:chemistry*; D004801:Eosine Yellowish-(YS) / Q000737:chemistry; D006801:Humans; D008027:Light; D000069349:Linezolid; D008958:Models, Molecular; D023303:Oxazolidinones / Q000097:blood / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D059629:Signal-To-Noise Ratio; D013057:Spectrum Analysis; D013607:Tablets
chemical_list: D000081:Acetamides; D002614:Chelating Agents; D023303:Oxazolidinones; D013607:Tablets; D010165:Palladium; D000069349:Linezolid; D004801:Eosine Yellowish-(YS)
keywords: Eosin; Frequency doubling scattering; Linezolid; Palladium acetate; Resonance Rayleigh scattering; Second-order scattering