title: Differentiation of chicken umbilical cord mesenchymal stem cells into beta-like pancreatic islet cells.
abstract: In this study, we explored the possibility of using in vitro differentiation to create functional beta-like islet cells from chicken umbilical cord mesenchymal stem cells (UCMSCs). Passaged UCMSCs were induced to differentiate into pancreatic beta-like islet cells. Differentiated cells were observed through dithizone staining, and Pdx1 and insulin expressed in differentiated cells were detected with immunofluorescence. Insulin and C-peptide production from differentiated cells were analyzed using ELISA and western blotting. Differentiated cells were found to not only express Pdx1, insulin, and C-peptide, but also to display a glucose-responsive secretion of these hormones.
authors.affiliation: College of Wildlife Resources, Northeast Forestry University , Harbin , P. R. China.
authors.forename: Chunyu
authors.initials: C
authors.lastname: Bai
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Gao
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Feng
authors.forename: Yanze
authors.initials: Y
authors.lastname: Yu
authors.forename: Gentong
authors.initials: G
authors.lastname: Meng
authors.forename: Minghai
authors.initials: M
authors.lastname: Zhang
authors.forename: Weijun
authors.initials: W
authors.lastname: Guan
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002469:Cell Separation; D002642:Chick Embryo; D002645:Chickens; D003584:Cytological Techniques / Q000379:methods*; D004230:Dithizone / Q000378:metabolism; D005786:Gene Expression Regulation; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D013194:Staining and Labeling; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D007328:Insulin; D004230:Dithizone
keywords: chicken; islet cells; mesenchymal stem cells; umbilical cord