title: Analytical assay platforms for soluble target engagement biomarkers: old favorites and emerging technologies.
abstract: Quantification of soluble biomarkers is critical for assessing target engagement in both preclinical and clinical settings. Since the advent of the commonly used ELISA, there has been an array of immunoassay platforms to achieve this goal, and many researchers will have a favorite. This review will compare and contrast immunoassay technologies used by many pharmaceutical and CRO groups and highlight emerging assay platforms that may deliver more sensitive assays. Several case studies will be used throughout as the changing immunoassay landscape is discussed.
authors.affiliation: Medimmune, Cambridge, CB21 6GH, UK.
authors.forename: Joanne
authors.initials: J
authors.lastname: Goodman
authors.forename: Balaji
authors.initials: B
authors.lastname: Agoram
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D055808:Drug Discovery / Q000295:instrumentation / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*
chemical_list: D015415:Biomarkers