title: Anticancer activity of Petroselinum sativum seed extracts on MCF-7 human breast cancer cells.
abstract: Pharmacological and preventive properties of Petroselinum sativum seed extracts are well known, but the anticancer activity of alcoholic extracts and oil of Petroselinum sativum seeds on human breast cancer cells have not been explored so far. Therefore, the present study was designed to investigate the cytotoxic activities of these extracts against MCF-7 cells. Cells were exposed to 10 to 1000 μg/ml of alcoholic seed extract (PSA) and seed oil (PSO) of Petroselinum sativum for 24 h. Post-treatment, percent cell viability was studied by 3-(4, 5-dimethylthiazol-2yl)-2, 5-biphenyl tetrazolium bromide (MTT) and neutral red uptake (NRU) assays, and cellular morphology by phase contrast inverted microscopy. The results showed that PSA and PSO significantly reduced cell viability, and altered the cellular morphology of MCF-7 cells in a concentration dependent manner. Concentrations of 50 μg/ml and above of PSA and 100 μg/ml and above of PSO were found to be cytotoxic in MCF-7 cells. Cell viability at 50, 100, 250, 500 and 1000 μg/ml of PSA was recorded as 81%, 57%, 33%, 8% and 5%, respectively, whereas at 100, 250, 500, and 1000 μg/ml of PSO values were 90%, 78%, 62%, and 8%, respectively by MTT assay. MCF-7 cells exposed to 250, 500 and 1000 μg/ml of PSA and PSO lost their typical morphology and appeared smaller in size. The data revealed that the treatment with PSA and PSO of Petroselinum sativum induced cell death in MCF-7 cells.
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia E-mail : nidachem@gmail.com.
authors.forename: Nida Nayyar
authors.initials: NN
authors.lastname: Farshori
authors.forename: Ebtesam Saad
authors.initials: ES
authors.lastname: Al-Sheddi
authors.forename: Mai Mohammad
authors.initials: MM
authors.lastname: Al-Oqail
authors.forename: Javed
authors.initials: J
authors.lastname: Musarrat
authors.forename: Abdulaziz Ali
authors.initials: AA
authors.lastname: Al-Khedhairy
authors.forename: Maqsood Ahmed
authors.initials: MA
authors.lastname: Siddiqui
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D028528:Petroselinum / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts