title: Developing and mature human granulocytes express ELP 6 in the cytoplasm.
abstract: BACKGROUND
  c3orf75 is a conserved open reading frame within the human genome and has recently been identified as the Elongator subunit, ELP6 [1]. The Elongator enzyme complex has diverse roles, including translational control, neuronal development, cell migration and tumorigenicity [2].
  
  
  OBJECTIVE
  To identify genes expressed early in human eosinophil development.
  
  
  METHODS
  Eosinophilopoiesis was investigated by gene profiling of IL-5 stimulated CD34+ cells; ELP6 mRNA is upregulated. A monoclonal antibody was raised to the recombinant protein predicted by the open reading frame.
  
  
  RESULTS
  ELP6 transcripts are upregulated in a human tissue culture model of eosinophil development during gene profiling experiments. Transcripts are expressed in most tissue types, as shown by reverse-transcriptase PCR. Western blot experiments show that human ELP6 is a 30 kDa protein expressed in the bone marrow, as well as in many other tissues. Flow cytometry experiments of human bone marrow mononuclear cells show that ELP6 is expressed intracellularly, in developing and mature human neutrophils, eosinophils and monocytes.
  
  
  CONCLUSIONS
  ELP6 is expressed intracellularly in developing and mature granulocytes and monocytes but not in lymphocytes and erythrocytes.
authors.affiliation: Department of Dermatology, University of Utah, Salt Lake City, UT, USA.
authors.forename: Lori A
authors.initials: LA
authors.lastname: Wagner
authors.forename: Shuping
authors.initials: S
authors.lastname: Wang
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Wayner
authors.forename: Clarissa
authors.initials: C
authors.lastname: Christensen
authors.forename: Sherrie J
authors.initials: SJ
authors.lastname: Perkins
authors.forename: Gavin W
authors.initials: GW
authors.lastname: Ward
authors.forename: Robert B
authors.initials: RB
authors.lastname: Weiss
authors.forename: Diane M
authors.initials: DM
authors.lastname: Dunn
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Redd
authors.forename: Gerald J
authors.initials: GJ
authors.lastname: Spangrude
authors.forename: Gerald J
authors.initials: GJ
authors.lastname: Gleich
mesh_terms: D018952:Antigens, CD34 / Q000235:genetics / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004804:Eosinophils / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005312:Fetal Blood / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D051548:Histone Acetyltransferases; D006801:Humans; D016130:Immunophenotyping; D015848:Interleukin-5 / Q000494:pharmacology; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; C576637:ELP6 protein, human; C508599:IL5 protein, human; D015848:Interleukin-5; D011506:Proteins; D012333:RNA, Messenger; D051548:Histone Acetyltransferases
keywords: ELP6; Granulopoiesis; antibody; bone marrow; human