title: Overexpression of periostin and lumican in esophageal squamous cell carcinoma.
abstract: To identify biomarkers for early detection for esophageal squamous cell carcinoma (ESCC), we previously carried out a genome-wide gene expression profiling study using an oligonucleotide microarray platform. This analysis led to identification of several transcripts that were significantly upregulated in ESCC compared to the adjacent normal epithelium. In the current study, we performed immunohistochemical analyses of protein products for two candidates genes identified from the DNA microarray analysis, periostin (POSTN) and lumican (LUM), using tissue microarrays. Increased expression of both periostin and lumican was observed in 100% of 137 different ESCC samples arrayed on tissue microarrays. Increased expression of periostin and lumican was observed in carcinoma as well as in stromal cell in the large majority of cases. These findings suggest that these candidates can be investigated in the sera of ESCC patients using ELISA or multiple reaction monitoring (MRM) type assays to further explore their utility as biomarkers.
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560066, India. rekha_v_kumar@yahoo.co.in.
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Kashyap
authors.forename: Arivusudar
authors.initials: A
authors.lastname: Marimuthu
authors.forename: Suraj
authors.initials: S
authors.lastname: Peri
authors.forename: Ghantasala S Sameer
authors.initials: GS
authors.lastname: Kumar
authors.forename: Harrys K C
authors.initials: HK
authors.lastname: Jacob
authors.forename: Thottethodi Subrahmanya Keshava
authors.initials: TS
authors.lastname: Prasad
authors.forename: Riaz
authors.initials: R
authors.lastname: Mahmood
authors.forename: K V Veerendra
authors.initials: KV
authors.lastname: Kumar
authors.forename: M Vijaya
authors.initials: MV
authors.lastname: Kumar
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Meltzer
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Montgomery
authors.forename: Rekha V
authors.initials: RV
authors.lastname: Kumar
authors.forename: Akhilesh
authors.initials: A
authors.lastname: Pandey