title: A Protein Phosphatase 1 involved in correct nucleus positioning in trypanosomes.
abstract: Reversible protein phosphorylation is a key regulator in intracellular functions. In the African trypanosome, Trypanosoma brucei, the serine-threonine phosphatase PP1-3, is localised in the cytoplasm. RNAi mediated knockdown of PP1-3 leads to a coordinated rearrangement of cellular organelles and compartments in the procyclic trypanosome. These parasites display their nucleus at the very posterior end of the cell. The kinetoplast is very close to the nucleus, and often located in a more anterior position. The lysosomal compartment, which in a normal procyclic cell is situated between nucleus and kinetoplast, is now positioned towards the anterior end of the cell. The Flagellum Attachment Zone, essential for cytokinesis, is still constructed, allowing initiation of the cleavage furrow and cell division. These adaptations allow dividing cells to distribute their organelles among the daughter cells and to proliferate normally. PP1-3 is therefore essential in conserving the intracellular organisation of the procyclic trypanosome cell.
authors.affiliation: MNHN/CNRS UMR7245, RDDM, Muséum National d'Histoire Naturelle, 61, rue Buffon, 75231 Paris 05, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Gallet
authors.affiliation: MNHN/CNRS UMR7245, RDDM, Muséum National d'Histoire Naturelle, 61, rue Buffon, 75231 Paris 05, France.
authors.forename: Raphaël
authors.initials: R
authors.lastname: Demonchy
authors.affiliation: MNHN/CNRS UMR7245, RDDM, Muséum National d'Histoire Naturelle, 61, rue Buffon, 75231 Paris 05, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Koppel
authors.affiliation: MNHN/CNRS UMR7245, RDDM, Muséum National d'Histoire Naturelle, 61, rue Buffon, 75231 Paris 05, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Grellier
authors.affiliation: MNHN/CNRS UMR7245, RDDM, Muséum National d'Histoire Naturelle, 61, rue Buffon, 75231 Paris 05, France. Electronic address: lkohl@mnhn.fr.
authors.forename: Linda
authors.initials: L
authors.lastname: Kohl
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism*; D048749:Cytokinesis; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D054645:Protein Phosphatase 1 / Q000235:genetics* / Q000378:metabolism*; D021381:Protein Transport; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000378:metabolism*
chemical_list: D054645:Protein Phosphatase 1
keywords: Nucleus; PP1; Phosphatase; Position; Trypanosome