title: Multivalent paediatric allergy vaccines protect against allergic anaphylaxis in mice.
abstract: BACKGROUND
  Almost a quarter of the world population suffers from IgE-mediated allergies. T cells and IgG-producing B cells can produce protection, but treatment for disease is laborious with unsatisfactory patient compliance.
  
  
  OBJECTIVE
  We sought to identify whether paediatric allergy vaccines affected later allergen sensitization and onset of disease when used prophylactically.
  
  
  METHODS
  A murine model of anaphylaxis was applied. Mice were first immunized with monovalent or multivalent allergy vaccines that also contained aluminium hydroxide and CpG oligodeoxynucleotide as adjuvants. Later, the mice were sensitized by multiple low-dose injections of aluminium-adsorbed allergen. After a dormant period, the mice were challenged systemically with high-dose allergen, and the clinical signs of anaphylaxis were recorded. Throughout the immunization and sensitization periods, blood was collected for serological testing.
  
  
  RESULTS
  Immunization with allergy vaccines produced antigen-specific protection against sensitization as measured by systemic anaphylaxis in mice. The long-term effect was observed both after juvenile (5-6 weeks) and neonatal (7 days) vaccination. Monovalent and pentavalent vaccines were protective to a similar level. Protection was associated with increased secretion of IgG2a and production of IFN-γ. Protection could also be transferred to sensitized mice via serum or via CD25-positive CD4 T cells.
  
  
  CONCLUSIONS
  Prophylactic and multivalent allergy vaccines in juvenile and neonatal mice protected against later sensitization and anaphylaxis. Such treatment may provide a rational measure for future management of allergen-related diseases and their strong socio-economic impact on daily life.
authors.affiliation: Department of Dermatology, University Hospital Zurich, Zurich, Switzerland.
authors.forename: Y
authors.initials: Y
authors.lastname: Waeckerle-Men
authors.forename: Y
authors.initials: Y
authors.lastname: Liang
authors.forename: S
authors.initials: S
authors.lastname: von Moos
authors.forename: T M
authors.initials: TM
authors.lastname: Kündig
authors.forename: P
authors.initials: P
authors.lastname: Johansen
mesh_terms: D019264:Adoptive Transfer; D000485:Allergens / Q000276:immunology; D000707:Anaphylaxis / Q000276:immunology* / Q000517:prevention & control*; D000818:Animals; D056738:Cross Protection / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007115:Immunization Schedule; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D051379:Mice; D010047:Ovalbumin / Q000009:adverse effects; D065631:Rhinitis, Allergic; D012221:Rhinitis, Allergic, Perennial / Q000517:prevention & control; D014612:Vaccines / Q000276:immunology*
chemical_list: D000485:Allergens; D007074:Immunoglobulin G; D014612:Vaccines; D007073:Immunoglobulin E; D010047:Ovalbumin
keywords: T cells; anaphylaxis; antibodies; childhood vaccine; murine model of allergy