title: Preparation and affinity-based purification of caged linear DNA for light-controlled gene expression in mammalian cells.
abstract: A facile and useful method for preparing caged DNAs was developed. The method includes a caging reaction of a linear dsDNA having a minimal sequence of protein expression with Bio-Bhc-diazo and affinity separation of the caged DNA. Effective suppression and photo-mediated restoration of protein expression were demonstrated.
authors.affiliation: Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan. furuta@biomol.sci.toho-u.ac.jp.
authors.forename: Aoi
authors.initials: A
authors.lastname: Teraoka
authors.forename: Kanako
authors.initials: K
authors.lastname: Murakoshi
authors.forename: Kyoko
authors.initials: K
authors.lastname: Fukamauchi
authors.forename: Akinobu Z
authors.initials: AZ
authors.lastname: Suzuki
authors.forename: Soichiro
authors.initials: S
authors.lastname: Watanabe
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Furuta
mesh_terms: D000818:Animals; D001391:Azo Compounds / Q000737:chemistry*; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D003374:Coumarins / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D008027:Light; D015394:Molecular Structure
chemical_list: C432678:6-bromo-4-diazomethyl-7-hydroxycoumarin; D001391:Azo Compounds; D003374:Coumarins; D004247:DNA