title: In vivo Tracking of Human Neural Stem Cells Following Transplantation into a Rodent Model of Ischemic Stroke.
abstract: OBJECTIVE
  Ischemic stroke caused by middle cerebral artery occlusion (MCAo) is the major type of stroke, but there are currently very limited options for cure. It has been shown that neural stem cells (NSCs) or neural precursor cells (NPCs) can survive and improve neurological deficits when they are engrafted in animal models of various neurological diseases. However, how the transplanted NSCs or NPCs are act in vivo in the injured or diseased brain is largely unknown. In this study, we utilized magnetic resonance imaging (MRI) techniques in order to understand the fates of human NSCs (HB1.F3) following transplantation into a rodent model of MCAo.
  
  
  RESULTS
  HB1.F3 human NSCs were pre-labeled with ferumoxides (Feridex(®))-protamine sulfate complexes, which were visualized and examined by MRI up to 9 weeks after transplantation. Migration of the transplanted cells to the infarct area was further confirmed by histological methods.
  
  
  CONCLUSIONS
  Based on these observations, we speculate that the transplanted NSCs have the extensive migratory ability to the injured site, which will in turn contribute to functional recovery in stroke.
authors.affiliation: CHA Stem Cell Institute, Department of Biomedical Science, CHA University, Seoul.
authors.forename: Da-Jeong
authors.initials: DJ
authors.lastname: Chang
authors.forename: Hyeyoung
authors.initials: H
authors.lastname: Moon
authors.forename: Yong Hyun
authors.initials: YH
authors.lastname: Lee
authors.forename: Nayeon
authors.initials: N
authors.lastname: Lee
authors.forename: Hong J
authors.initials: HJ
authors.lastname: Lee
authors.forename: Iksoo
authors.initials: I
authors.lastname: Jeon
authors.forename: Hyunseung
authors.initials: H
authors.lastname: Lee
authors.forename: Tae-Sun
authors.initials: TS
authors.lastname: Hwang
authors.forename: Seung-Hun
authors.initials: SH
authors.lastname: Oh
authors.forename: Dong Ah
authors.initials: DA
authors.lastname: Shin
authors.forename: Seung U
authors.initials: SU
authors.lastname: Kim
authors.forename: Kwan Soo
authors.initials: KS
authors.lastname: Hong
authors.forename: Jihwan
authors.initials: J
authors.lastname: Song
keywords: Feridex; Human neural stem cells (hNSCs); In vivo tracking; Ischemic stroke; Magnetic resonance imaging (MRI); Middle cerebral artery occlusion (MCAo)