title: Oral immunization with recombinant Lactobacillus acidophilus expressing the adhesin Hp0410 of Helicobacter pylori induces mucosal and systemic immune responses.
abstract: Helicobacter pylori infection is relatively common worldwide and is closely related to gastric mucosa-associated lymphoid tissue (MALT) lymphoma, chronic gastritis, and stomach ulcers. Therefore, a safe and effective method for preventing H. pylori infection is urgently needed. Given that developing an effective vaccine against H. pylori is one of the best alternatives, H. pylori adhesin Hp0410 was expressed in the food-grade bacterium Lactobacillus acidophilus. The recombinant live bacterial vaccine was then used to orally vaccinate mice, and the immunoprotective effects of Hp0410-producing strains were investigated. H. pylori colonization in the stomach of mice immunized with the recombinant L. acidophilus was significantly reduced, in comparison with that in control groups. Furthermore, mucosal secretory IgA antibodies were elicited in the mucosal tissue of mice immunized with the recombinant bacteria, and specific anti-Hp0410 IgG responses were also detected in mouse serum. There was a significant increase in the level of protection against gastric Helicobacter infection following a challenge with H. pylori Sydney strain 1 (SS1). Our results collectively indicate that adhesin Hp0410 is a promising candidate vaccine antigen, and recombinant L. acidophilus expressing Hp0410 is likely to constitute an effective, low-cost, live bacterial vaccine against H. pylori.
authors.affiliation: The School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Fan
authors.initials: F
authors.lastname: Hongying
authors.forename: Wu
authors.initials: W
authors.lastname: Xianbo
authors.forename: Yu
authors.initials: Y
authors.lastname: Fang
authors.forename: Bai
authors.initials: B
authors.lastname: Yang
authors.forename: Long
authors.initials: L
authors.lastname: Beiguo
mesh_terms: D018829:Adhesins, Bacterial / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000097:blood*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000008:administration & dosage*; D005260:Female; D005822:Genetic Vectors; D016481:Helicobacter Infections / Q000517:prevention & control; D006801:Humans; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000032:analysis; D007779:Lactobacillus acidophilus / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D004337:Drug Carriers; D007070:Immunoglobulin A; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; C073899:adhesin, helicobacter