title: Analysis of cyathane-type diterpenoids from Cyathus striatus and Hericium erinaceus by high-resolution MALDI MS imaging.
abstract: Fungal secondary metabolites in both fruiting bodies and pellets from submerged cultures of basidiomycetes were analyzed by atmospheric pressure matrix-assisted laser desorption/ionization-mass spectrometry imaging at a lateral resolution of 15 μm, a mass resolution of 140,000 at m/z 200 and a mass accuracy of better than 2 ppm. The striatals A, B, C, and D, and a number of erinacine type metabolites were detected in the basidiomycetes Cyathus striatus and Hericium erinaceus, respectively. The two fungi were selected as model species, as they are well-known for efficient production of terpenoid secondary metabolites with interesting biological activities, e.g., antibacterial, fungicidal, cytotoxic properties, and stimulating effects on nerve growth factor synthesis. The localization of metabolites revealed a mostly homogeneous distribution of the striatals in the pellets of C. striatus, while a concentration gradient, increasing to the center, was observed in the pellets of H. erinaceus. A mostly homogeneous distribution of metabolites was also found in the fruiting body of H. erinaceus.
authors.affiliation: Institute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, Schubertstrasse 60, building 16, 35392, Giessen, Germany.
authors.forename: Dhaka Ram
authors.initials: DR
authors.lastname: Bhandari
authors.forename: Tian
authors.initials: T
authors.lastname: Shen
authors.forename: Andreas
authors.initials: A
authors.lastname: Römpp
authors.forename: Holger
authors.initials: H
authors.lastname: Zorn
authors.forename: Bernhard
authors.initials: B
authors.lastname: Spengler
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D055365:Cyathus / Q000737:chemistry*; D004224:Diterpenes / Q000737:chemistry*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D015394:Molecular Structure; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004224:Diterpenes; C497675:cyathane