title: Preventive effect of tert-butylhydroquinone on scrotal heat-induced damage in mouse testes.
abstract: To investigate the effect of tert-butylhydroquinone (tBHQ) on scrotal heat-induced damage in mice testes, 8-week-old mice were divided into 6 groups and administered with or without tBHQ through diet (10 mg/g), intraperitoneal injection (100 mg/kg body weight), or intratestis injection (12.5 mg/kg body weight), respectively. After single scrotal heat exposure (42 °C for 25 min), trunk blood and testes were collected 48 h later. The testes from diet and intraperitoneal tBHQ-treated mice showed more compact interstitial cells and less germ cell loss in the seminiferous epithelium compared with their corresponding non-tBHQ groups. However, intratestis tBHQ treatment showed no marked difference relative to the non-treatment group. In addition, pre-treatment of tBHQ caused lower testosterone concentrations and reduced expression of cytochrome P450 17α-hydroxylase/17,20-lyase (CYP 17) compared to the corresponding non-tBHQ groups. The results indicated that scrotal heat-induced structural damage was partly prevented by pre-treatment of tBHQ, which could be used as an effective antioxidant for preventing scrotal heat-mediated male infertility.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Y S
authors.initials: YS
authors.lastname: Li
authors.forename: Y G
authors.initials: YG
authors.lastname: Piao
authors.forename: K
authors.initials: K
authors.lastname: Nagaoka
authors.forename: G
authors.initials: G
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Taya
authors.forename: C M
authors.initials: CM
authors.lastname: Li
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D006358:Hot Temperature / Q000009:adverse effects*; D006873:Hydroquinones / Q000494:pharmacology* / Q000627:therapeutic use; D007248:Infertility, Male / Q000209:etiology / Q000517:prevention & control*; D008297:Male; D051379:Mice; D013094:Spermatozoa / Q000187:drug effects; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013739:Testosterone / Q000097:blood
chemical_list: D000975:Antioxidants; D006873:Hydroquinones; D013739:Testosterone; C018855:2-tert-butylhydroquinone; D013254:Steroid 17-alpha-Hydroxylase