title: An optics-based variable-temperature assay system for characterizing thermodynamics of biomolecular reactions on solid support.
abstract: A biological state is equilibrium of multiple concurrent biomolecular reactions. The relative importance of these reactions depends on physiological temperature typically between 10 °C and 50 °C. Experimentally the temperature dependence of binding reaction constants reveals thermodynamics and thus details of these biomolecular processes. We developed a variable-temperature opto-fluidic system for real-time measurement of multiple (400-10,000) biomolecular binding reactions on solid supports from 10 °C to 60 °C within ±0.1 °C. We illustrate the performance of this system with investigation of binding reactions of plant lectins (carbohydrate-binding proteins) with 24 synthetic glycans (i.e., carbohydrates). We found that the lectin-glycan reactions in general can be enthalpy-driven, entropy-driven, or both, and water molecules play critical roles in the thermodynamics of these reactions.
authors.affiliation: Department of Physics, University of California, One Shields Avenue, Davis, California 95616, USA.
authors.forename: Yiyan
authors.initials: Y
authors.lastname: Fei
authors.forename: James P
authors.initials: JP
authors.lastname: Landry
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Li
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.forename: Kam
authors.initials: K
authors.lastname: Lau
authors.forename: Shengshu
authors.initials: S
authors.lastname: Huang
authors.forename: Harshal A
authors.initials: HA
authors.lastname: Chokhawala
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: X D
authors.initials: XD
authors.lastname: Zhu
mesh_terms: D008958:Models, Molecular; D055096:Optical Devices; D037121:Plant Lectins / Q000737:chemistry / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D013696:Temperature; D013816:Thermodynamics; D013997:Time Factors; D014025:Titanium / Q000737:chemistry
chemical_list: D037121:Plant Lectins; D011134:Polysaccharides; D014025:Titanium