title: Motility characteristics are altered for Rickettsia bellii transformed to overexpress a heterologous rickA gene.
abstract: The rickettsial protein RickA activates host cell factors associated with the eukaryotic actin cytoskeleton and is likely involved with rickettsial host cell binding and infection and the actin-based motility of spotted fever group rickettsiae. The rickA gene sequence and protein vary substantially between Rickettsia species, as do observed motility-associated phenotypes. To help elucidate the function of RickA and determine the effects of species-specific RickA variations, we compared extracellular binding, intracellular motility, and intercellular spread phenotypes of three Rickettsia bellii variants. These included two shuttle vector-transformed R. bellii strains and the wild-type isolate from which they were derived, R. bellii RML 369C. Both plasmid shuttle vectors carried spectinomycin resistance and a GFPuv reporter; one contained Rickettsia monacensis-derived rickA, and the other lacked the rickA gene. Rickettsia bellii transformed to express R. monacensis rickA highly overexpressed this transcript in comparison to its native rickA. These rickettsiae also moved at higher velocities and followed a more curved path than the negative-control transformants. A lower proportion of R. monacensis rickA-expressing bacteria ever became motile, however, and they formed smaller plaques.
authors.affiliation: University of Minnesota, Department of Entomology, St. Paul, Minnesota, USA.
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Oliver
authors.forename: Nicole Y
authors.initials: NY
authors.lastname: Burkhardt
authors.forename: Roderick F
authors.initials: RF
authors.lastname: Felsheim
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Kurtti
authors.forename: Ulrike G
authors.initials: UG
authors.lastname: Munderloh
mesh_terms: D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D017353:Gene Deletion; D015870:Gene Expression; D005822:Genetic Vectors; D008124:Locomotion; D012281:Rickettsia / Q000235:genetics / Q000502:physiology*; D014169:Transformation, Bacterial
chemical_list: D001426:Bacterial Proteins; C491314:RickA protein, Rickettsia