title: Acute and chronic mu opioids differentially regulate thrombospondins 1 and 2 isoforms in astrocytes.
abstract: Chronic opioids induce synaptic plasticity, a major neuronal adaptation. Astrocyte activation in synaptogenesis may play a critical role in opioid tolerance, withdrawal, and dependence. Thrombospondins 1 and 2 (TSP1/2) are astrocyte-secreted matricellular glycoproteins that promote neurite outgrowth as well as dendritic spine and synapse formation, all of which are inhibited by chronic μ opioids. In prior studies, we discovered that the mechanism of TSP1 regulation by μ opioids in astrocytes involves crosstalk between three different classes of receptors, μ opioid receptor, EGFR and TGFβR. Moreover, TGFβ1 stimulated TSP1 expression via EGFR and ERK/MAPK activation, indicating that EGFR is a signaling hub for opioid and TGFβ1 actions. Using various selective antagonists, and inhibitors, here we compared the mechanisms of chronic opioid regulation of TSP1/2 isoform expression in vivo and in immortalized rat cortical astrocytes. TSP1/2 release from astrocytes was also monitored. Acute and chronic μ opioids, morphine, and the prototypic μ ligand, DAMGO, modulated TSP2 protein levels. TSP2 but not TSP1 protein content was up-regulated by acute (3 h) morphine or DAMGO by an ERK/MAPK dependent mechanism. Paradoxically, TSP2 protein levels were altered neither by TGFβ1 nor by astrocytic neurotrophic factors, EGF, CNTF, and BMP4. TSP1/2 immunofluorescence was increased in astrocytes subjected to scratch-wounding, suggesting TSPs may be useful markers for the "reactive" state of these cells and potentially for different types of injury. Previously, we determined that chronic morphine attenuated both neurite outgrowth and synapse formation in cocultures of primary astrocytes and neurons under similar temporal conditions that μ opioids reduced TSP1 protein levels in astrocytes. Here we found that, after the same 8 day treatment, morphine or DAMGO diminished TSP2 protein levels in astrocytes. Therefore, μ opioids may deter synaptogenesis via both TSP1/2 isoforms, but by distinct mechanisms.
authors.affiliation: E. A. Doisy Department of Biochemistry and Molecular Biology, St. Louis University of Medicine , 1100 S. Grand Blvd., St. Louis, Missouri, 63104, United States.
authors.forename: Ellen
authors.initials: E
authors.lastname: Phamduong
authors.forename: Maanjot K
authors.initials: MK
authors.lastname: Rathore
authors.forename: Nicholas R
authors.initials: NR
authors.lastname: Crews
authors.forename: Alexander S
authors.initials: AS
authors.lastname: D'Angelo
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Leinweber
authors.forename: Pranay
authors.initials: P
authors.lastname: Kappera
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Krenning
authors.forename: Victoria R
authors.initials: VR
authors.lastname: Rendell
authors.forename: Mariana M
authors.initials: MM
authors.lastname: Belcheva
authors.forename: Carmine J
authors.initials: CJ
authors.lastname: Coscia
mesh_terms: D000701:Analgesics, Opioid / Q000494:pharmacology*; D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D055415:Bone Morphogenetic Protein 4 / Q000494:pharmacology; D020934:Ciliary Neurotrophic Factor / Q000494:pharmacology; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)- / Q000494:pharmacology*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000187:drug effects / Q000378:metabolism; D046228:Microarray Analysis; D009020:Morphine / Q000494:pharmacology*; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D019700:Thrombospondin 1 / Q000187:drug effects* / Q000378:metabolism; D019699:Thrombospondins / Q000187:drug effects* / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology; D000099262:Thrombospondin 2
chemical_list: D000701:Analgesics, Opioid; D055415:Bone Morphogenetic Protein 4; D020934:Ciliary Neurotrophic Factor; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D004815:Epidermal Growth Factor; D048049:Extracellular Signal-Regulated MAP Kinases; D009020:Morphine; D020033:Protein Isoforms; D012333:RNA, Messenger; D019700:Thrombospondin 1; D019699:Thrombospondins; D053773:Transforming Growth Factor beta1; D000099262:Thrombospondin 2