title: Preparing size markers for gel electrophoresis.
abstract: Here we present two simple methods for preparing radiolabeled size markers for gel electrophoresis. The first procedure describes the generation of an RNA marker ladder by the alkaline hydrolysis of (32)P 5'- or 3'-end-labeled RNA. The second procedure describes the labeling of DNA fragments produced by digestion of pBR322 with the restriction enzyme MspI.
authors.forename: Timothy W
authors.initials: TW
authors.lastname: Nilsen
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004586:Electrophoresis / Q000379:methods* / Q000592:standards*; D007553:Isotope Labeling; D008970:Molecular Weight; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D012015:Reference Standards
chemical_list: D012313:RNA; D004247:DNA