title: Prdm6 is essential for cardiovascular development in vivo.
abstract: Members of the PRDM protein family have been shown to play important roles during embryonic development. Previous in vitro and in situ analyses indicated a function of Prdm6 in cells of the vascular system. To reveal physiological functions of Prdm6, we generated conditional Prdm6-deficient mice. Complete deletion of Prdm6 results in embryonic lethality due to cardiovascular defects associated with aberrations in vascular patterning. However, smooth muscle cells could be regularly differentiated from Prdm6-deficient embryonic stem cells and vascular smooth muscle cells were present and proliferated normally in Prdm6-deficient embryos. Conditional deletion of Prdm6 in the smooth muscle cell lineage using a SM22-Cre driver line resulted in perinatal lethality due to hemorrhage in the lungs. We thus identified Prdm6 as a factor that is essential for the physiological control of cardiovascular development.
authors.affiliation: Institut für Klinische Chemie und Pathobiochemie, Klinikum Rechts der Isar, Technische Universität München, Munich, Germany ; German Cancer Consortium (DKTK), Heidelberg, Germany ; German Cancer Research Center (DKFZ), Heidelberg, Germany ; Laboratory of Signaling in the Immune System, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Gewies
authors.forename: Mercedes
authors.initials: M
authors.lastname: Castineiras-Vilarino
authors.forename: Uta
authors.initials: U
authors.lastname: Ferch
authors.forename: Nina
authors.initials: N
authors.lastname: Jährling
authors.forename: Katja
authors.initials: K
authors.lastname: Heinrich
authors.forename: Ulrike
authors.initials: U
authors.lastname: Hoeckendorf
authors.forename: Gerhard K H
authors.initials: GK
authors.lastname: Przemeck
authors.forename: Matthias
authors.initials: M
authors.lastname: Munding
authors.forename: Olaf
authors.initials: O
authors.lastname: Groß
authors.forename: Timm
authors.initials: T
authors.lastname: Schroeder
authors.forename: Marion
authors.initials: M
authors.lastname: Horsch
authors.forename: E Loraine
authors.initials: EL
authors.lastname: Karran
authors.forename: Aneela
authors.initials: A
authors.lastname: Majid
authors.forename: Stefan
authors.initials: S
authors.lastname: Antonowicz
authors.forename: Johannes
authors.initials: J
authors.lastname: Beckers
authors.forename: Martin
authors.initials: M
authors.lastname: Hrabé de Angelis
authors.forename: Hans-Ulrich
authors.initials: HU
authors.lastname: Dodt
authors.forename: Christian
authors.initials: C
authors.lastname: Peschel
authors.forename: Irmgard
authors.initials: I
authors.lastname: Förster
authors.forename: Martin J S
authors.initials: MJ
authors.lastname: Dyer
authors.forename: Jürgen
authors.initials: J
authors.lastname: Ruland
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D019521:Body Patterning; D002319:Cardiovascular System / Q000196:embryology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D017931:DNA Primers; D051379:Mice; D018345:Mice, Knockout; D009130:Muscle, Smooth / Q000166:cytology; D018919:Neovascularization, Physiologic; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D017931:DNA Primers; C509647:PRDM6, protein, mouse; D012097:Repressor Proteins