title: Loss of ASAP3 destabilizes cytoskeletal protein ACTG1 to suppress cancer cell migration.
abstract: ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), previously known as ACAP4, DDEFL1 and UPLC1, is considered to be an important regulator in cancer cell migration/invasion and actin-based cytoskeletal remodeling. However, the underlying mechanisms through which ASAP3 mediates these processes are not well-elucidated. This study reported that in certain types of cancer cells, loss of ASAP3 suppressed cell migration/invasion, in part by destabilizing γ-actin-1 (ACTG1), a cytoskeletal protein considered to be an integral component of the cell migratory machinery, essential for the rearrangement of the dynamic cytoskeletal networks and important in diseases, such as brain malformation, hearing loss and cancer development. The data, for the first time, link ASAP3 with ACTG1 in the regulation of cytoskeletal maintenance and cell motility.
authors.affiliation: School of Nursing, The Third Military Medical University, Chongqing 400038, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Biology, School of Life Sciences, Xiamen University, Xiang'an, Xiamen, Fujian 361102, P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Kong
authors.affiliation: State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Biology, School of Life Sciences, Xiamen University, Xiang'an, Xiamen, Fujian 361102, P.R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Biology, School of Life Sciences, Xiamen University, Xiang'an, Xiamen, Fujian 361102, P.R. China.
authors.forename: Dahan
authors.initials: D
authors.lastname: Chen
authors.affiliation: School of Biomedical Sciences, Huaqiao University, Quanzhou, Fujian 362021, P.R. China.
authors.forename: Qiuyan
authors.initials: Q
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Biology, School of Life Sciences, Xiamen University, Xiang'an, Xiamen, Fujian 361102, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: School of Biomedical Sciences, Huaqiao University, Quanzhou, Fujian 362021, P.R. China.
authors.forename: Ruian
authors.initials: R
authors.lastname: Xu
authors.affiliation: School of Nursing, The Third Military Medical University, Chongqing 400038, P.R. China.
authors.forename: Xianyuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Biology, School of Life Sciences, Xiamen University, Xiang'an, Xiamen, Fujian 361102, P.R. China.
authors.forename: James Y
authors.initials: JY
authors.lastname: Yang
mesh_terms: D000199:Actins / Q000235:genetics*; D002465:Cell Movement / Q000235:genetics*; D003598:Cytoskeletal Proteins / Q000378:metabolism; D020690:GTPase-Activating Proteins / Q000235:genetics* / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D000199:Actins; D003598:Cytoskeletal Proteins; D020690:GTPase-Activating Proteins; C488555:ASAP3 protein, human