title: Mussel-inspired protein-mediated surface functionalization of electrospun nanofibers for pH-responsive drug delivery.
abstract: pH-responsive drug delivery systems could mediate drug releasing rate by changing the pH values at specific times as per the pathophysiological need of the disease. This paper demonstrates that a mussel-inspired protein polydopamine coating can tune the loading and releasing rate of charged molecules from electrospun poly(ε-caprolactone) (PCL) nanofibers in solutions with different pH values. In vitro release profiles show that the positive charged molecules release significantly faster in acidic than those in neutral and basic environments within the same incubation time. The results of fluorescein diacetate staining and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays show the viability of cancer cells after treatment with doxorubicin-released media at different pH values qualitatively and quantitatively, indicating that the media containing doxorubicin that were released in solutions at low pH values could kill a significantly higher number of cells than those released in solutions at high pH values. Together, the pH-responsive drug delivery systems based on polydopamine-coated PCL nanofibers could have potential application in the oral delivery of anticancer drugs for treating gastric cancer and in vaginal delivery of anti-viral drugs or anti-inflammatory drugs, which could raise their efficacy, deliver them to the specific target and minimize their toxic side effects.
authors.affiliation: Marshall Institute for Interdisciplinary Research and Center for Diagnostic Nanosystems, Marshall University, Huntington, WV 25755, USA.
authors.forename: J
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Marshall Institute for Interdisciplinary Research and Center for Diagnostic Nanosystems, Marshall University, Huntington, WV 25755, USA. Electronic address: xiej@marshall.edu.
authors.forename: J
authors.initials: J
authors.lastname: Xie
authors.affiliation: Marshall Institute for Interdisciplinary Research and Center for Diagnostic Nanosystems, Marshall University, Huntington, WV 25755, USA.
authors.forename: B
authors.initials: B
authors.lastname: Ma
authors.affiliation: Marshall Institute for Interdisciplinary Research and Center for Diagnostic Nanosystems, Marshall University, Huntington, WV 25755, USA.
authors.forename: D E
authors.initials: DE
authors.lastname: Bartlett
authors.affiliation: Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Sciences, Chinese Academy of Sciences, Hefei 230031, China.
authors.forename: A
authors.initials: A
authors.lastname: Xu
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, 117576 , Singapore.
authors.forename: C-H
authors.initials: CH
authors.lastname: Wang
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007211:Indoles / Q000737:chemistry; D007700:Kinetics; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012235:Rhodamines / Q000494:pharmacology
chemical_list: D007211:Indoles; D011091:Polyesters; D011108:Polymers; D011506:Proteins; D012235:Rhodamines; C568283:polydopamine; C026188:rhodamine 6G; C016240:polycaprolactone; D004317:Doxorubicin
keywords: Drug delivery; Electrospun fibers; Poly(ε-caprolactone); Polydopamine coating; pH sensitive