title: High-performance liquid chromatography separation of small molecules on a porous poly (trimethylol propane triacrylate-co-N-isopropylacrylamide-co-ethylene dimethacrylate) monolithic column.
abstract: A porous monolith was prepared by in situ free-radical polymerization using N-isopropylacrylamide (NIPAAm) and trimethylol propane triacrylate (TMPTA) as functional monomers, ethylene dimethacrylate (EDMA) as crosslinking agent. The chemical group of the monolith was assayed by a Fourier transform infrared spectroscopy (FT-IR) method and the morphology of optimized monolithic column was characterized by scanning electron microscopy (SEM). The mechanical strength and permeability have been studied in detail as well. The run-to-run and column-to-column reproducibility of the retention times were less than 0.9% and 3.0%, respectively. Furthermore, the influence of temperature and mobile phase composition on the separation of aromatic compounds was investigated. The results indicated that poly (trimethylol propane triacrylate-co-N-isopropylacrylamide-co-ethylenedimethacrylate) (TMPTA-co-NIPAAm-co-EDMA) monolithic column not only had high porosity and strong rigidity, but also was a promising tool for analyzing small molecule compounds with a short analysis time by controlling the column temperature.
authors.affiliation: College of Pharmacy, Hebei University, Hebei Province Key Laboratory of Pharmaceutical Quality Control, Baoding 071002, China. Electronic address: lhy1610@126.com.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Pharmacy, Hebei University, Hebei Province Key Laboratory of Pharmaceutical Quality Control, Baoding 071002, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Bai
authors.affiliation: College of Pharmacy, Hebei University, Hebei Province Key Laboratory of Pharmaceutical Quality Control, Baoding 071002, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wei
authors.affiliation: College of Pharmacy, Hebei University, Hebei Province Key Laboratory of Pharmaceutical Quality Control, Baoding 071002, China.
authors.forename: Gengliang
authors.initials: G
authors.lastname: Yang
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000179:Acrylates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006358:Hot Temperature; D008689:Methacrylates / Q000737:chemistry*; D064347:Microscopy, Electrochemical, Scanning; D058105:Polymerization; D016062:Porosity; D015203:Reproducibility of Results; D054852:Small Molecule Libraries / Q000302:isolation & purification*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000178:Acrylamides; D000179:Acrylates; D008689:Methacrylates; D054852:Small Molecule Libraries; C027993:trimethylolpropane triacrylate; C004919:ethylene dimethacrylate; C067295:N-isopropylacrylamide
keywords: High performance liquid chromatography; Monolithic column; N-isopropylacrylamide; Small molecule compounds