title: Homogeneous phenomenon of the graft when using different genotype characteristic of recipients/donors in living donor liver transplantation.
abstract: OBJECTIVE
  To investigate the evidence of homogeneous phenomenon on CYP3A5*3 MDR1-3435 and CYP3A4*18 of the liver graft after living donor liver transplantation (LDLT).
  
  
  METHODS
  We identified the proportional change of the CYP3A5*3, MDR1-3435 and CYP3A4*18 from the peripheral blood mononuclear cell of 41 pairs recipient/donor with different genotype polymorphisms and 119 liver graft biopsy samples used with the pyrosequencing technique after LDLT. Polymerase chain reaction/ligase detection reaction assay and restriction fragment length polymorphism was employed for genotyping the CYP3A5*3 and CYP3A4*18 single nucleotide polymorphisms (SNPs). All of the recipients and donors expressed with the similar SNP genotype of CYP3A5*3, MDR1-3435 or CYP3A4*18 were excluded.
  
  
  RESULTS
  The final genetic polymorphisms of the liver graft biopsy samples of CYP3A5*3, MDR1-3435 and CYP3A4*18 was predominated depends on the donor with restriction fragment length polymorphism and seems to be less related to the recipient. The proportional changes of G to A alleles of the 119 samples of CYP3A5*3 (included A > A/G, A/G > A, A/G > G, G > A, G > A/G and A > G), C to T alleles of the 108 samples of MDR1-3435 (included C > C/T, C/T > C, C/T > T, T > C/T and T > C), and T to C alleles of 15 samples of CYP3A4*18 (included T/C > T and T > C/T) were significant different between the recipients and the liver graft biopsy samples (P < 0.0001) and less difference when compared with the donors in the pyrosequencing analysis after LDLT.
  
  
  CONCLUSIONS
  The CYP3A5*3, MDR1-3435 and CYP3A4*18 of the recipient could be modified by the donor so-called homogenous phenomenon when the recipient's blood drained into the liver graft.
authors.affiliation: King-Wah Chiu, Toshiaki Nakano, Kuang-Den Chen, Li-Wen Hsu, Chia-Yun Lai, Ho-Ching Chiu, Ching-Yin Huang, Yu-Fan Cheng, Shigeru Goto, Chao-Long Chen, Liver Transplant Program, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 833, Taiwan.
authors.forename: King-Wah
authors.initials: KW
authors.lastname: Chiu
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Nakano
authors.forename: Kuang-Den
authors.initials: KD
authors.lastname: Chen
authors.forename: Li-Wen
authors.initials: LW
authors.lastname: Hsu
authors.forename: Chia-Yun
authors.initials: CY
authors.lastname: Lai
authors.forename: Ho-Ching
authors.initials: HC
authors.lastname: Chiu
authors.forename: Ching-Yin
authors.initials: CY
authors.lastname: Huang
authors.forename: Yu-Fan
authors.initials: YF
authors.lastname: Cheng
authors.forename: Shigeru
authors.initials: S
authors.lastname: Goto
authors.forename: Chao-Long
authors.initials: CL
authors.lastname: Chen
keywords: CYP3A4*18; CYP3A5*3; Liver biopsy; Living donor liver transplantation; MDR1-3435; Pyrosequencing