title: Reproductive toxicity on female mice induced by microcystin-LR.
abstract: There are increasing concerns about health risk of human exposure to microcystin-LR (MC-LR) over the past few decades. Nevertheless, its female mammalian reproductive toxicity has not been addressed yet. In the present study, we firstly reported MC-LR could impact female reproductive function of mammals. After 28 days MC-LR exposure, relative ovary weight significantly reduced in 20 μg/kg MC-LR group and this reduction may be relative with pathomorphological changes of ovary. The result of histological evaluation of follicles showed that primordial follicles decreased roughly in half at high dose level compared with control. Since serum hormone assay indicated that MC-LR induced decrease of progesterone but not FSH or LH, disturbance of estrus cycle was seemed to result from direct impact of ovary rather than indirectly from hypothalamus or pituitary. As expected, MC-LR was detected in the ovaries of MC-LR exposure mice by immunoblot analysis.
authors.affiliation: Immunology and Reproductive Biology Laboratory, Medical School, Nanjing University, Nanjing 210093, China; Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing 210093, China; State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, 210093, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Wu
authors.affiliation: Immunology and Reproductive Biology Laboratory, Medical School, Nanjing University, Nanjing 210093, China; Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing 210093, China; State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, 210093, China.
authors.forename: Sijia
authors.initials: S
authors.lastname: Shao
authors.affiliation: Immunology and Reproductive Biology Laboratory, Medical School, Nanjing University, Nanjing 210093, China; Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing 210093, China; State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, 210093, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhou
authors.affiliation: Immunology and Reproductive Biology Laboratory, Medical School, Nanjing University, Nanjing 210093, China; Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing 210093, China; State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, 210093, China.
authors.forename: Siyuan
authors.initials: S
authors.lastname: Wen
authors.affiliation: Immunology and Reproductive Biology Laboratory, Medical School, Nanjing University, Nanjing 210093, China; Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing 210093, China; State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, 210093, China.
authors.forename: Fu
authors.initials: F
authors.lastname: Chen
authors.affiliation: Immunology and Reproductive Biology Laboratory, Medical School, Nanjing University, Nanjing 210093, China; Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing 210093, China; State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, 210093, China. Electronic address: hanxd@nju.edu.cn.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Han
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000493:pharmacokinetics / Q000633:toxicity*; D030762:Estrous Cycle / Q000187:drug effects; D005260:Female; D008387:Marine Toxins / Q000493:pharmacokinetics / Q000633:toxicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D052998:Microcystins / Q000493:pharmacokinetics / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D011374:Progesterone / Q000097:blood; D012098:Reproduction
chemical_list: D001427:Bacterial Toxins; D008387:Marine Toxins; D052998:Microcystins; D011374:Progesterone; C057862:cyanoginosin LR
keywords: MC-LR; Ovary; Reproductive toxicity