title: Optimization of enzyme-assisted extraction and characterization of polysaccharides from Hericium erinaceus.
abstract: The enzyme-assisted extraction (EAE) of polysaccharides from the fruits of Hericium erinaceus was studied. In this study, response surface methodology and the Box-Behnken design based on single-factor and orthogonal experiments were applied to optimize the EAE conditions. The optimal extraction conditions were as follows: a pH of 5.71, a temperature of 52.03°C and a time of 33.79 min. The optimal extraction conditions resulted in the highest H. erinaceus polysaccharides (HEP) yield, with a value 13.46 ± 0.37%, which represented an increase of 67.72% compared to hot water extraction (HWE). The polysaccharides were characterized by FT-IR, SEM, CD, AFM, and GC. The results showed that HEP was composed of mannose, glucose, xylose, and galactose in a molar ratio of 15.16:5.55:4.21:1. The functional groups of the H. erinaceus polysaccharides extracted by HWE and EAE were fundamentally identical but had apparent conformational changes.
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhu
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Guanghua
authors.initials: G
authors.lastname: Mao
authors.forename: Ye
authors.initials: Y
authors.lastname: Zou
authors.forename: Weiwei
authors.initials: W
authors.lastname: Feng
authors.forename: Daheng
authors.initials: D
authors.lastname: Zheng
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Lulu
authors.initials: L
authors.lastname: Zhou
authors.forename: Tianxiu
authors.initials: T
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Liuqing
authors.initials: L
authors.lastname: Yang
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Wu
mesh_terms: D001487:Basidiomycota / Q000737:chemistry*; D005591:Chemical Fractionation / Q000379:methods*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D015233:Models, Statistical; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D013696:Temperature
chemical_list: D011134:Polysaccharides; D006026:Glycoside Hydrolases
keywords: Characterization; Enzyme-assisted extraction; Hericium erinaceus polysaccharides; Optimization; Response surface methodology