title: Topical dual-stain difference imaging for rapid intra-operative tumor identification in fresh specimens.
abstract: Assessing tumor margin status during surgery is critical to ensure complete resection of cancer tissue; however, current approaches are ineffective and often result in repeat surgery. We present an optical imaging approach for margin assessment using topical application of two fluorescent stains, one targeted to a tumor biomarker and the other a nontargeted reference, to freshly excised specimens. Computing a normalized difference image from fluorescence images of the targeted and untargeted stains suppresses the confounding effects of nonspecific uptake. Applying this approach in excised breast tumor models produced promising tumor-to-normal tissue contrasts that were significantly higher than single-targeted-stain imaging.
authors.forename: Scott C
authors.initials: SC
authors.lastname: Davis
authors.forename: Summer L
authors.initials: SL
authors.lastname: Gibbs
authors.forename: Jason R
authors.initials: JR
authors.lastname: Gunn
authors.forename: Brian W
authors.initials: BW
authors.lastname: Pogue
mesh_terms: D000273:Adipose Tissue / Q000473:pathology; D000287:Administration, Topical; D000818:Animals; D005456:Fluorescent Dyes / Q000008:administration & dosage* / Q000378:metabolism*; D007432:Intraoperative Period; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology* / Q000601:surgery*; D051379:Mice; D061848:Optical Imaging / Q000379:methods*; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes