title: Immobilization of bile salt hydrolase enzyme on mesoporous SBA-15 for co-precipitation of cholesterol.
abstract: We describe herein a simple and effective strategy for immobilization of bile salt hydrolase enzyme by grafting glutaraldehyde groups inside channels of APTES functionalized SBA-15. The increase in glutaraldehyde concentration prevents leakage of enzyme but showed a steep decrease in enzyme activity in the immobilized matrix. So the degree of cross-linking should be the minimum possible to ensure sufficient stability without loss of activity. Cross-linking carried out with 0.1% glutaraldehyde concentration showed the highest activity, so this was used in all further experiments. Physico-chemical characterizations of the immobilized enzyme were carried out by XRD, N2 adsorption, TEM, FTIR and (29)Si CP-MAS NMR techniques. Immobilized BSH exhibits enhanced stability over a wide pH (3-11) and temperature range (40-80 °C) and retains an activity even after recycling experiments and six months of storage. From our in vivo research experiment toward co-precipitation of cholesterol, we have shown that immobilized BSH enzyme may be the promising catalyst for the reduction of serum cholesterol levels in our preliminary investigation. Enhancement in pH stability at the extreme side of pH may favor the use of immobilized BSH enzyme for drug delivery purpose to with stand extreme pH conditions in the gastrointestinal conditions.
authors.affiliation: Catalysis Division, National Chemical Laboratory, Pune 411008, India. Electronic address: bhangepallavi@yahoo.in.
authors.forename: Pallavi
authors.initials: P
authors.lastname: Bhange
authors.affiliation: Biochemical Sciences Division, National Chemical Laboratory, Pune 411008, India.
authors.forename: N
authors.initials: N
authors.lastname: Sridevi
authors.affiliation: Department of Chemistry, Shivaji University, Kolhapur 416004, Maharashtra, India.
authors.forename: Deu S
authors.initials: DS
authors.lastname: Bhange
authors.affiliation: Biochemical Sciences Division, National Chemical Laboratory, Pune 411008, India.
authors.forename: Asmita
authors.initials: A
authors.lastname: Prabhune
authors.affiliation: Catalysis Division, National Chemical Laboratory, Pune 411008, India.
authors.forename: Veda
authors.initials: V
authors.lastname: Ramaswamy
mesh_terms: D000327:Adsorption; D000581:Amidohydrolases / Q000737:chemistry* / Q000378:metabolism; D002384:Catalysis; D002784:Cholesterol / Q000737:chemistry*; D016503:Drug Delivery Systems; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D055550:Protein Stability; D012822:Silicon Dioxide / Q000737:chemistry; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; C509969:SBA-15; D012822:Silicon Dioxide; D002784:Cholesterol; D000581:Amidohydrolases; C023154:choloylglycine hydrolase; D005976:Glutaral
keywords: Bile salt hydrolase; Co-precipitation of cholesterol; SBA-15