title: Isolation of DNA using magnetic nanoparticles coated with dimercaptosuccinic acid.
abstract: Lately, the isolation of DNA using magnetic nanoparticles has received increased attention owing to their facile manipulation and low costs. Although methods involving their magnetic separation have been extensively studied, there is currently a need for an efficient technique to isolate DNA for highly sensitive diagnostic applications. We describe herein a method to isolate and purify DNA using biofunctionalized superparamagnetic nanoparticles synthesized by a modified polyol method to obtain the desired monodispersity, followed by surface modification with meso-2,3-dimercaptosuccinic acid (DMSA) containing carboxyl groups for DNA absorption. The DMSA-coated magnetic nanoparticles (DMSA-MNPs) were used for the isolation of DNA, with a maximum yield of 86.16%. In particular, we found that the isolation of DNA using small quantities of DMSA-MNPs was much more efficient than that using commercial microbeads (NucliSENS-easyMAG, BioMérieux). Moreover, the DMSA-MNPs were successfully employed in the isolation of genomic DNA from human blood. In addition, the resulting DNA-nanoparticle complex was directly subjected to PCR amplification without prior elution, which could eventually lead to simple, rapid, sensitive and integrated diagnostic systems.
authors.affiliation: Department of Materials Science and Engineering, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-713, Republic of Korea; Pioneer Research Center for Biomedical Nanocrystals, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-713, Republic of Korea.
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Min
authors.affiliation: Pioneer Research Center for Biomedical Nanocrystals, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-713, Republic of Korea; Department of Laboratory Medicine, College of Medicine, Korea University Guro Hospital, Seoul 152-703, Republic of Korea.
authors.forename: Mi-Kyung
authors.initials: MK
authors.lastname: Woo
authors.affiliation: Department of Materials Science and Engineering, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-713, Republic of Korea; Pioneer Research Center for Biomedical Nanocrystals, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-713, Republic of Korea.
authors.forename: Ha Young
authors.initials: HY
authors.lastname: Yoon
authors.affiliation: Pioneer Research Center for Biomedical Nanocrystals, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-713, Republic of Korea; Department of Laboratory Medicine, College of Medicine, Korea University Guro Hospital, Seoul 152-703, Republic of Korea.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Jang
authors.affiliation: Pioneer Research Center for Biomedical Nanocrystals, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-713, Republic of Korea.
authors.forename: Jun Hua
authors.initials: JH
authors.lastname: Wu
authors.affiliation: Pioneer Research Center for Biomedical Nanocrystals, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-713, Republic of Korea; Department of Laboratory Medicine, College of Medicine, Korea University Guro Hospital, Seoul 152-703, Republic of Korea. Electronic address: malarim@korea.ac.kr.
authors.forename: Chae-Seung
authors.initials: CS
authors.lastname: Lim
authors.affiliation: Department of Materials Science and Engineering, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-713, Republic of Korea; Pioneer Research Center for Biomedical Nanocrystals, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-713, Republic of Korea. Electronic address: ykim97@korea.ac.kr.
authors.forename: Young Keun
authors.initials: YK
authors.lastname: Kim
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D004247:DNA / Q000302:isolation & purification*; D006801:Humans; D059346:Magnets / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D004113:Succimer / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D004247:DNA; D004113:Succimer
keywords: DNA isolation; Dimercaptosuccinic acid; Magnetic nanoparticle; Superparamagnetism