title: Rescue of a recombinant Machupo virus from cloned cDNAs and in vivo characterization in interferon (αβ/γ) receptor double knockout mice.
abstract: Machupo virus (MACV) is the etiological agent of Bolivian hemorrhagic fever (BHF), a reemerging and neglected tropical disease associated with high mortality. The prototypical strain of MACV, Carvallo, was isolated from a human patient in 1963, but minimal in vitro and in vivo characterization has been reported. To this end, we utilized reverse genetics to rescue a pathogenic MACV from cloned cDNAs. The recombinant MACV (rMACV) had in vitro growth properties similar to those of the parental MACV. Both viruses caused similar disease development in alpha/beta and gamma interferon receptor knockout mice, including neurological disease development and high mortality. In addition, we have identified a novel murine model with mortality and neurological disease similar to BHF disease reported in humans and nonhuman primates.
authors.affiliation: Galveston National Laboratory, Department of Pathology, Sealy Vaccine Center, University of Texas Medical Branch, Galveston, Texas, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Patterson
authors.forename: Alexey
authors.initials: A
authors.lastname: Seregin
authors.forename: Cheng
authors.initials: C
authors.lastname: Huang
authors.forename: Olga
authors.initials: O
authors.lastname: Kolokoltsova
authors.forename: Jennifer
authors.initials: J
authors.lastname: Smith
authors.forename: Milagros
authors.initials: M
authors.lastname: Miller
authors.forename: Jeanon
authors.initials: J
authors.lastname: Smith
authors.forename: Nadezhda
authors.initials: N
authors.lastname: Yun
authors.forename: Allison
authors.initials: A
authors.lastname: Poussard
authors.forename: Ashley
authors.initials: A
authors.lastname: Grant
authors.forename: Bersabeh
authors.initials: B
authors.lastname: Tigabu
authors.forename: Aida
authors.initials: A
authors.lastname: Walker
authors.forename: Slobodan
authors.initials: S
authors.lastname: Paessler
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D006477:Arenaviruses, New World / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D004195:Disease Models, Animal; D006478:Hemorrhagic Fever, American / Q000235:genetics*; D006652:Histological Techniques; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D053633:Receptor, Interferon alpha-beta / Q000172:deficiency / Q000235:genetics; D017471:Receptors, Interferon / Q000172:deficiency / Q000235:genetics; D059386:Reverse Genetics / Q000379:methods; D017423:Sequence Analysis, RNA; D014709:Vero Cells; D000096743:Interferon gamma Receptor
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D053633:Receptor, Interferon alpha-beta; D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor