title: Rapid identification of potential drought tolerance genes from Solanum tuberosum by using a yeast functional screening method.
abstract: Identification of major stress tolerance genes of a crop plant is important for the rapid development of its stress-tolerant cultivar. Here, we used a yeast functional screen method to identify potential drought-tolerance genes from a potato plant. A cDNA expression library was constructed from hyperosmotic stressed potato plants. The yeast transformants expressing different cDNAs were selected for their ability to survive in hyperosmotic stress conditions. The relative tolerances of the selected yeast transformants to multiple abiotic stresses were also studied. Specific potato cDNAs expressed in the tolerant yeast transformants were identified. Sixty-nine genes were found capable of enhancing hyperosmotic stress tolerance of yeast. Based on the relative tolerance data generated, 12 genes were selected, which could be most effective in imparting higher drought tolerance to potato with better survival in salt and high-temperature stresses. Orthologues of few genes identified here are previously known to increase osmotic stress tolerance of yeast and plants; however, specific studies are needed to confirm their role in the osmotic stress tolerance of potato.
authors.affiliation: Department of Molecular Biotechnology, Konkuk University, Seoul 143 701, South Korea.
authors.forename: Sajeesh
authors.initials: S
authors.lastname: Kappachery
authors.forename: Jae Woong
authors.initials: JW
authors.lastname: Yu
authors.forename: Gangadhar
authors.initials: G
authors.lastname: Baniekal-Hiremath
authors.forename: Se Won
authors.initials: SW
authors.lastname: Park
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics* / Q000502:physiology*; D003001:Cloning, Molecular; D003470:Culture Media; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D055864:Droughts; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005686:Galactokinase / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors; D006358:Hot Temperature; D009997:Osmotic Pressure / Q000502:physiology; D010957:Plasmids / Q000235:genetics / Q000502:physiology; D018749:RNA, Plant / Q000096:biosynthesis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D011198:Solanum tuberosum / Q000235:genetics* / Q000502:physiology*; D014170:Transformation, Genetic
chemical_list: D003470:Culture Media; D018076:DNA, Complementary; D018749:RNA, Plant; D029701:Saccharomyces cerevisiae Proteins; C524219:GAL1 protein, S cerevisiae; D005686:Galactokinase
keywords: Drought; Solanum tuberosum; Tolerance genes; Yeast functional screening; cDNA library