title: The last intron of the human thrombopoietin gene enhances expression in milk of transgenic mice.
abstract: Introns can enhance gene expression levels. This effect is known as intron-mediated enhancement, which is different from that of enhancers or promoters. In our previous study, under the control of the cytomegalovirus or goat β-casein promoter, the vector containing intron V-TPOcDNA expressed the highest thrombopoietin (TPO) level, whereas the vector containing TPOgDNA expressed the lowest level. In order to verify whether intron V also improves TPO expression in the milk of transgenic mice, rat whey acidic protein promoter was used as regulatory element to construct mammary gland expression vectors including pTPOWA (containing TPOcDNA), pTPOWB (containing intron V-TPOcDNA), and pTPOWC (containing TPOgDNA). These vectors were transfected into HC-11 cells and the supernatants were analyzed at 48 h. The highest TPO level was found in pTPOWB (795 pg/mL) and the lowest level in pTPOWC (193 pg/mL). Then, corresponding vectors were microinjected into fertilized mice zygotes. Transgenic mice were identified by polymerase chain reaction and Southern blot. Enzyme-linked immunosorbent assay was performed to measure TPO levels in the milk of lactating transgenic mice. The highest and lowest TPO levels were found in transgenic mice carrying intron V-TPOcDNA (2,307 pg/mL) and in transgenic mice carrying TPOgDNA (242 pg/mL), respectively. Thus, intron V remarkably improved TPO expression in transgenic mice.
authors.affiliation: School of Biotechnology, Southern Medical University, North1838 Guangzhou Road, Guangzhou, 510515, People's Republic of China, liyan_nys@hotmail.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Mingqian
authors.initials: M
authors.lastname: Zhou
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhou
authors.forename: Yunshan
authors.initials: Y
authors.lastname: Ning
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D006801:Humans; D007438:Introns; D008321:Mammary Glands, Animal; D008533:Megakaryocytes / Q000187:drug effects; D051379:Mice; D008822:Mice, Transgenic; D008892:Milk / Q000378:metabolism; D008894:Milk Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D013926:Thrombopoietin / Q000235:genetics* / Q000494:pharmacology
chemical_list: D008894:Milk Proteins; C024471:whey acidic proteins; D013926:Thrombopoietin