title: Baicalin scavenges reactive oxygen species and protects human keratinocytes against UVC-induced cytotoxicity.
abstract: Long-term exposure to solar ultraviolet (UV) radiation can cause multiple skin disorders, including skin cancer. Protection against UV-induced damage is, therefore, a worldwide concern. Baicalin, a major component of traditional Chinese medicine Scutellaria baicalensis, has been reported to have antioxidant and cytostatic effects on normal epithelial and normal peripheral blood and myeloid cells. In the current study, we examined whether baicalin could also effectively protect human keratinocytes from damaging short-wave UVC irradiation. Baicalin-scavenged reactive oxygen species increased within 2 h after UVC radiation. Baicalin also abrogated UVC-induced apoptosis. In addition, we identified the major products after UVC radiation with T4 UV endonuclease, finding that baicalin prevented cyclobutane pyrimidine dimer formation induced by UVC. Furthermore, baicalin also prevented formation of oxidative adducts induced by UVC. Our results demonstrated the utility of baicalin in assessing the potential contribution of traditional Chinese medicinal agents in therapy of UVC-induced genomic damage to skin and suggest potential application of these agents as pharmaceuticals in prevention of solar-induced skin damage.
authors.affiliation: Department of Medical Imaging and Radiological Science, Central Taiwan University of Science and Technology, No. 666, Buzih Road, Beiten District, Taichung 40605, Taiwan, R.O.C. sslin@ctust.edu.tw; artbau2@gmail.com.
authors.forename: Shou-Cheng
authors.initials: SC
authors.lastname: Wang
authors.forename: Sue-Fung
authors.initials: SF
authors.lastname: Chen
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: Lee
authors.forename: Chin-Liang
authors.initials: CL
authors.lastname: Chuang
authors.forename: Da-Tian
authors.initials: DT
authors.lastname: Bau
authors.forename: Song-Shei
authors.initials: SS
authors.lastname: Lin
mesh_terms: D017209:Apoptosis; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D005419:Flavonoids / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D011740:Pyrimidine Dimers / Q000235:genetics; D011836:Radiation Tolerance / Q000187:drug effects; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D005419:Flavonoids; D016166:Free Radical Scavengers; D011740:Pyrimidine Dimers; D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; C038044:baicalin
keywords: Baicalin; UV; keratinocytes; reactive oxygen species