title: Adsorption of NGF and BDNF derived peptides on gold surfaces.
abstract: This study tackles the interaction between gold surfaces and two peptide fragments named NGF(1-14) and BDNF(1-12), able to mimic the proliferative activity of the nerve growth factor (NGF) and the brain derived neurotrophic factor (BDNF), respectively. The physical adsorption processes on the solid surface from both single and binary peptide solutions, at physiological and acid pH, were investigated by QCM-D and CD experiments, as well as by molecular dynamics calculations. The relevant physicochemical properties at the hybrid bio-interface, including peptide-surface interaction, conformational changes, hydrodynamic thickness, viscoelastic parameters, and competitive vs. synergic behaviour of the two peptide fragments towards the surface, were scrutinized. Biological assays with neuronal cells pointed to the maintenance of the biological activity of NGF(1-14) and BDNF(1-12) peptide molecules within the adlayers on the gold surface.
authors.affiliation: Department of Pharmaceutical Sciences, University of Catania, Viale Andrea Doria, 6, I-95125, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Forte
authors.forename: Alessio
authors.initials: A
authors.lastname: Travaglia
authors.forename: Antonio
authors.initials: A
authors.lastname: Magrì
authors.forename: Cristina
authors.initials: C
authors.lastname: Satriano
authors.forename: Diego
authors.initials: D
authors.lastname: La Mendola
mesh_terms: D000327:Adsorption; D019208:Brain-Derived Neurotrophic Factor / Q000737:chemistry*; D045744:Cell Line, Tumor; D002942:Circular Dichroism; D006046:Gold / Q000737:chemistry*; D006801:Humans; D056004:Molecular Dynamics Simulation; D015394:Molecular Structure; D020932:Nerve Growth Factor / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D058525:Quartz Crystal Microbalance Techniques; D013499:Surface Properties
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D010455:Peptides; D006046:Gold; D020932:Nerve Growth Factor