title: High-density distributed electrode network, a multi-functional electroporation method for delivery of molecules of different sizes.
abstract: We present a multi-functional electroporation method for delivery of biomolecule utilizing a high-density distributed electrode network (HDEN) under tri-phase electric stimulation. The HDEN device, with which drastic pH change during the electroporation was avoided,was demonstrated to be highly effective for transfection of not only DNA plasmids and small interfering RNAs (siRNA), but also a small molecular anti-cancer drug, into cells in adjustable volumes of cell suspension. The method constitutes a very flexible electroporation approach in a wide range of in vitro or ex vivo scenarios in various tubes, standard multi-well plates as well as flow chambers.
authors.affiliation: 1] Institute of Microelectronics, National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Peking University, Beijing 100871, China [2].
authors.forename: Mengxi
authors.initials: M
authors.lastname: Wu
authors.forename: Deyao
authors.initials: D
authors.lastname: Zhao
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Zhong
authors.forename: Hao
authors.initials: H
authors.lastname: Yan
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Wang
authors.forename: Zicai
authors.initials: Z
authors.lastname: Liang
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Li
mesh_terms: D016475:3T3 Cells; D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D045744:Cell Line, Tumor; D003412:Cricetulus; D004247:DNA / Q000378:metabolism; D004285:Dogs; D004566:Electrodes; D018274:Electroporation / Q000379:methods*; D018922:HL-60 Cells; D006367:HeLa Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D061986:MCF-7 Cells; D061985:Madin Darby Canine Kidney Cells; D051379:Mice; D010957:Plasmids / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D014162:Transfection / Q000379:methods*
chemical_list: D034741:RNA, Small Interfering; D004247:DNA