title: Weissella confusa Cab3 dextransucrase: properties and in vitro synthesis of dextran and glucooligosaccharides.
abstract: Food-derived Weissella spp. have gained attention during recent years as efficient dextran producers. Weissella confusa Cab3 dextransucrase (WcCab3-DSR) was isolated applying PEG fractionation and used for in vitro synthesis of dextran and glucooligosaccharides. WcCab3-DSR had a molar mass of 178 kDa and was activated by Co(2+) and Ca(2+) ions. Glycerol and Tween 80 enhanced enzyme stability, and its half-life at 30°C increased from 10h to 74 h and 59 h, respectively. The (1)H and (13)C NMR spectral analysis of the produced dextran confirmed the presence of main chain α-(1→6) linkages with only 3.0% of α-(1→3) branching, of which some were elongated. An HPSEC analysis in DMSO revealed a high molecular weight of 1.8 × 10(7)g/mol. Glucooligosaccarides produced through the acceptor reaction with maltose, were analyzed with HPAEC-PAD and ESI-MS/MS. They were a homologous series of isomaltooligosaccharides with reducing end maltose units. To the best of our knowledge, this is a first report on native W. confusa dextransucrase.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India.
authors.forename: Shraddha
authors.initials: S
authors.lastname: Shukla
authors.forename: Qiao
authors.initials: Q
authors.lastname: Shi
authors.forename: Ndegwa H
authors.initials: NH
authors.lastname: Maina
authors.forename: Minna
authors.initials: M
authors.lastname: Juvonen
authors.lastname: Maijatenkanen
authors.forename: Arun
authors.initials: A
authors.lastname: Goyal
mesh_terms: D060326:Chemistry Techniques, Synthetic; D003911:Dextrans / Q000138:chemical synthesis* / Q000737:chemistry; D005964:Glucosyltransferases / Q000378:metabolism*; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D013696:Temperature; D058836:Weissella / Q000201:enzymology*
chemical_list: D003911:Dextrans; D009844:Oligosaccharides; D005964:Glucosyltransferases; C020025:dextransucrase
keywords: Dextran; Dextransucrase; Glucooligosaccharides; Weissella confusa