title: Green and rapid synthesis of anticancerous silver nanoparticles by Saccharomyces boulardii and insight into mechanism of nanoparticle synthesis.
abstract: Rapidly developing field of nanobiotechnology dealing with metallic nanoparticle (MNP) synthesis is primarily lacking control over size, shape, dispersity, yield, and reaction time. Present work describes an ecofriendly method for the synthesis of silver nanoparticles (AgNPs) by cell free extract (CFE) of Saccharomyces boulardii. Parameters such as culture age (stationary phase growth), cell mass concentration (400 mg/mL), temperature (35°C), and reaction time (4 h), have been optimized to exercise a control over the yield of nanoparticles and their properties. Nanoparticle (NP) formation was confirmed by UV-Vis spectroscopy, elemental composition by EDX (energy dispersive X-rays) analysis, and size and shape by transmission electron microscopy. Synthesized nanoparticles had the size range of 3-10 nm with high negative zeta potential (-31 mV) indicating excellent stability. Role of proteins/peptides in NP formation and their stability were also elucidated. Finally, anticancer activity of silver nanoparticles as compared to silver ions was determined on breast cancer cell lines.
authors.affiliation: Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, SAS Nagar, Punjab 160062, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Kaler
authors.forename: Sanyog
authors.initials: S
authors.lastname: Jain
authors.forename: Uttam Chand
authors.initials: UC
authors.lastname: Banerjee
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry*; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D055772:Green Chemistry Technology / Q000379:methods*; D006801:Humans; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology / Q000379:methods*; D012440:Saccharomyces / Q000378:metabolism*; D012834:Silver / Q000008:administration & dosage / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D012834:Silver