title: Jellyfish collagen stimulates production of TNF-α and IL-6 by J774.1 cells through activation of NF-κB and JNK via TLR4 signaling pathway.
abstract: We previously reported that jellyfish collagen stimulates both the acquired and innate immune responses. In the acquired immune response, jellyfish collagen enhanced immunoglobulin production by lymphocytes in vitro and in vivo. Meanwhile, in the innate immune response jellyfish collagen promoted cytokine production and phagocytotic activity of macrophages. The facts that jellyfish collagen plays several potential roles in stimulating cytokine production by macrophages have further attracted us to uncover its mechanisms. We herein describe that the cytokine production-stimulating activity of jellyfish collagen was canceled by a Toll-like receptor 4 (TLR4) inhibitor. Moreover, jellyfish collagen stimulated phosphorylation of inhibitor of κBα (IκBα), promoted the translocation of nucleus factor-κB (NF-κB), and activated c-Jun N-terminal kinase (JNK). A JNK inhibitor also abrogated the cytokine production-stimulating activity of jellyfish collagen. These results suggest that jellyfish collagen may facilitate cytokine production by macrophages through activation of NF-κB and JNK via the TLR4 signaling pathways.
authors.affiliation: Faculty of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan.
authors.forename: Agus Budiawan Naro
authors.initials: AB
authors.lastname: Putra
authors.affiliation: Faculty of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Nishi
authors.affiliation: Marutomo Co., Ltd., Iyo, Ehime 799-3192, Japan.
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Shiraishi
authors.affiliation: Marutomo Co., Ltd., Iyo, Ehime 799-3192, Japan.
authors.forename: Mikiharu
authors.initials: M
authors.lastname: Doi
authors.affiliation: Faculty of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan; South Ehime Fisheries Research Center, Ehime University, Ainan, Ehime 798-4205, Japan; Food and Health Sciences Research Center, Ehime University, Matsuyama, Ehime 790-8566, Japan. Electronic address: mars95@agr.ehime-u.ac.jp.
authors.forename: Takuya
authors.initials: T
authors.lastname: Sugahara
mesh_terms: D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D002460:Cell Line; D003094:Collagen / Q000276:immunology* / Q000494:pharmacology; D004731:Endotoxins / Q000276:immunology; D020672:I-kappa B Proteins / Q000378:metabolism; D015850:Interleukin-6 / Q000096:biosynthesis; D048031:JNK Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000276:immunology*; D010766:Phosphorylation; D037401:Scyphozoa / Q000276:immunology / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D000873:Anthracenes; D004731:Endotoxins; D020672:I-kappa B Proteins; D015850:Interleukin-6; D016328:NF-kappa B; C000606205:Nfkbia protein, mouse; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; C577686:interleukin-6, mouse; D000072000:NF-KappaB Inhibitor alpha; C432165:pyrazolanthrone; D003094:Collagen; D048031:JNK Mitogen-Activated Protein Kinases
keywords: Collagen; Cytokine; FBS; HRP; IFN; IL; JNK; LPS; MAPK; Macrophage; NF-κB; PRR; SD; TLR; TLR4; TNF; Toll-like receptor; c-Jun N-terminal kinases; fetal bovine serum; horseradish peroxidase; interferon; interleukin; lipopolysaccharide; mitogen-activated protein kinase; pattern-recognition receptor; standard deviation; tumor necrosis factor