title: Expression analysis of histone acetyltransferases in rice under drought stress.
abstract: Histone acetylation is one of the vital reversible modifications of chromatin structure that regulates gene expression in eukaryotes. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) maintain the homeostasis of histone acetylation. Studies in Arabidopsis have revealed that HATs are involved in plant responses to various stresses including light, temperature, salt and ABA. Drought stress, a very common environmental stress, could cause a range of physiological and biochemical responses in plants involving HATs. Eight HATs in four different families (CBP, GNAT, MYST, and TAF(II)250 family) are known in rice. In this research, four OsHATs, one from each family, were chosen based on in silico domain and promoter analysis for their response under drought conditions. Drought stress was introduced to two-leaf-stage rice seedlings. The effectiveness of drought treatment was confirmed by the measurement of relative water content (RWC). Real-time quantitative polymerase chain reaction analysis demonstrated that drought stress caused a significant increase in the expression of four HATs (OsHAC703, OsHAG703, OsHAF701 and OsHAM701) in rice plants. Additionally, the Western-blot analysis showed that the acetylation level on certain lysine sites of H3 (lysine 9, lysine 18 and lysine 27) and H4 (lysine 5) increased with OsHATs expression. The significant increase in the transcript levels of OsHATs and the acetylation level of lysine residues on Histone H3 and H4 suggest that OsHATs are involved in drought stress responses in rice.
authors.affiliation: Department of Biology, The University of Western Ontario, London, Ontario N6A 5B7, Canada; Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, London, Ontario N5V 4T3, Canada.
authors.forename: Hui
authors.initials: H
authors.lastname: Fang
authors.affiliation: Center for Translational Medicine, Guangxi Medical University, Nanning, Guangxi 530021, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Biology, The University of Western Ontario, London, Ontario N6A 5B7, Canada.
authors.forename: Greg
authors.initials: G
authors.lastname: Thorn
authors.affiliation: Key Laboratory of South China Agricultural Plant Genetics and Breeding, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Duan
authors.affiliation: Department of Biology, The University of Western Ontario, London, Ontario N6A 5B7, Canada; Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, London, Ontario N5V 4T3, Canada. Electronic address: lining.tian@agr.gc.ca.
authors.forename: Lining
authors.initials: L
authors.lastname: Tian
mesh_terms: D000064:Acclimatization / Q000502:physiology*; D000107:Acetylation; D055864:Droughts; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D051548:Histone Acetyltransferases / Q000378:metabolism*; D012275:Oryza / Q000502:physiology*; D013312:Stress, Physiological / Q000502:physiology*
chemical_list: D051548:Histone Acetyltransferases
keywords: Drought stress; Histone acetylation; Histone acetyltransferase; Rice