title: The effect of resistance training on plasma S1P level and gene expression of S1P1,2,3 receptors in male Wistar rats.
abstract: OBJECTIVE
  The purpose of present study was to study the effect of 8 weeks resistance training on plasma Sphingosine-1-phosphate (S1P) level and gene expression of S1P receptors in skeletal muscles of male Wistar rat.
  
  
  METHODS
  In this study 24 (8 week-old) male Wistar rats (190-250 gr) were divided randomly to a control (N.=12) and a training (N.=12) group. Resistance ladder was 1 meter height with 2 cm grid ladder. The content of plasma S1P and relative mRNA expression of S1P receptors were determined by high pressure liquid chromatography (HPLC) and Real-time PCR, respectively.
  
  
  RESULTS
  Resistance training increased the content of plasma S1P of exercised group compared to control group (P=0.001). Furthermore, Resistance exercise training increased the gene expression of S1P1 (P=0.001), S1P2 (P=0.000) and S1P3 receptors (P=0.021) in exercised flexor hallucis longus (FHL) compared to control group. In soleus (SOL) muscle, resistance training increased the gene expression of S1P1 (P=0.000), S1P2 (P=0.603) and S1P3 receptors (P=0.009).
  
  
  CONCLUSIONS
  The key conclusion is that resistance training strongly caused to increase in plasma S1P content and its receptors in skeletal muscles of rat that might indicate to the involvement of S1P signalling in the molecular events controlling adaptations of resistance trained muscles which needs to be elucidated in future studies.
authors.affiliation: Department of Sport Sciences, Tarbiat Modares University, Tehran, Iran - ghara_re@modares.ac.ir.
authors.forename: E
authors.initials: E
authors.lastname: Banitalebi
authors.forename: R
authors.initials: R
authors.lastname: Gharakhanlou
authors.forename: K
authors.initials: K
authors.lastname: Ghatrehsamani
authors.forename: A H
authors.initials: AH
authors.lastname: Parnow
authors.forename: H
authors.initials: H
authors.lastname: Teimori
authors.forename: M
authors.initials: M
authors.lastname: Mohammad Amoli
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D015870:Gene Expression; D006614:Hindlimb; D008246:Lysophospholipids / Q000097:blood*; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D049349:Receptors, Lysosphingolipid / Q000235:genetics / Q000378:metabolism*; D012016:Reference Values; D015203:Reproducibility of Results; D055070:Resistance Training; D012680:Sensitivity and Specificity; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D008246:Lysophospholipids; D049349:Receptors, Lysosphingolipid; C060506:sphingosine 1-phosphate; D013110:Sphingosine