title: A fraction containing kaempferol-3,4'-dimethylether from Larrea divaricata Cav. induces macrophage activation on mice infected with Candida albicans.
abstract: Larrea divaricata Cav. is a plant growing in South America. Both the infusion and a derived fraction (F1) of L. divaricata have proved to have immunomodulatory properties. Moreover, F1 can activate macrophages obtained from mice infected with Candida albicans. In this work, F1 was administrated to infected animals, and the state and type of activation of resident macrophages were studied. Results showed that F1 was able to activate macrophages obtained from infected mice by both classical and alternative pathways, probably by inducing a translocation of nuclear factor kappa-B. F1 increases not only the lysosomal activity of macrophages but also the production of phagosomal superoxide anion as a consequence of the activation of the Nicotinamide adenine dinucleotide phosphate oxidase (NADPH oxidase) complex. F1 induced an increase in the macrophage capacity to kill the fungus, which was reflected in a decrease in the levels of colonization of organs. A main flavonoid, kaempferol-3,4'-dimethylether, was identified in F1 by HPLC. This compound increased in vitro production of nitric oxide in heat-killed C. albicans-stimulated macrophages. The flavonoid could thus be considered one of the responsible molecules mediating the overall effects of F1 on the immune system in infected animals.
authors.affiliation: Microbiology Department, Faculty of Chemistry, Biochemistry and Pharmacy, National University of San Luis, San Luis, Argentina; Department of Pharmacology, IQUIMEFA-CONICET, Buenos Aires University, Buenos Aires, Argentina.
authors.forename: Renzo
authors.initials: R
authors.lastname: Martino
authors.forename: Fernando
authors.initials: F
authors.lastname: Canale
authors.forename: Valeria
authors.initials: V
authors.lastname: Sülsen
authors.forename: Rosario
authors.initials: R
authors.lastname: Alonso
authors.forename: Roberto
authors.initials: R
authors.lastname: Davicino
authors.forename: Aida
authors.initials: A
authors.lastname: Mattar
authors.forename: Claudia
authors.initials: C
authors.lastname: Anesini
authors.forename: Blas
authors.initials: B
authors.lastname: Micalizzi
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000187:drug effects*; D002177:Candidiasis / Q000276:immunology; D005260:Female; D044949:Kaempferols / Q000494:pharmacology*; D032442:Larrea / Q000737:chemistry*; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D044949:Kaempferols; D010936:Plant Extracts; C000590632:kaempferol-3,4'-dimethylether
keywords: Candida albicans; Larrea divaricata; kaempferol-3,4′-dimethylether; macrophages