title: Cross-talk between E. coli strains and a human colorectal adenocarcinoma-derived cell line.
abstract: Although there is great interest in the specific mechanisms of how gut microbiota modulate the biological processes of the human host, the extent of host-microbe interactions and the bacteria-specific metabolic activities for survival in the co-evolved gastrointestinal environment remain unclear. Here, we demonstrate a comprehensive comparison of the host epithelial response induced by either a pathogenic or commensal strain of Escherichia coli using a multi-omics approach. We show that Caco-2 cells incubated with E. coli display an activation of defense response genes associated with oxidative stress. Indeed, in the bacteria co-culture system, the host cells experience an altered environment compared with the germ-free system that includes reduced pH, depletion of major energy substrates, and accumulation of fermentation by-products. Measurement of intracellular Caco-2 cell metabolites revealed a significantly increased lactate concentration, as well as changes in TCA cycle intermediates. Our results will lead to a deeper understanding of acute microbial-host interactions.
authors.affiliation: Department of Nutrition, University of California, Davis, CA 95616-5270, USA.
authors.forename: Xuan
authors.initials: X
authors.lastname: He
authors.forename: Darya O
authors.initials: DO
authors.lastname: Mishchuk
authors.forename: Jigna
authors.initials: J
authors.lastname: Shah
authors.forename: Bart C
authors.initials: BC
authors.lastname: Weimer
authors.forename: Carolyn M
authors.initials: CM
authors.lastname: Slupsky
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology*; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D002952:Citric Acid Cycle / Q000502:physiology; D018920:Coculture Techniques / Q000379:methods; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology*; D004926:Escherichia coli / Q000378:metabolism / Q000502:physiology*; D054884:Host-Pathogen Interactions / Q000502:physiology*; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism
chemical_list: D019344:Lactic Acid