title: Nucleotide sequence of a cDNA clone of Brassica napus 12S storage protein shows homology with legumin from Pisum sativum.
abstract: The most abundant protein in seeds of Brassica napus (L.) is cruciferin, a legumin-like 12S storage protein. By in vitro translation of embryo RNA, and pulse-chase labelling of cultured embryos with (14)C-leucine, we have shown that the 30 kd α polypeptides and 20 kd β polypeptides of cruciferin are synthesized as a family of 50 kd precursors which are cleaved post-translationally. One member of the cruciferin family was cloned from embryo cDNA and sequenced. The nucleotide sequence of the cruciferin cDNA clone, pC1, contains one long open reading frame, which originates in a hydrophobic signal peptide region. Therefore, the complete sequence of the cruciferin mRNA was obtained by primer extension of the cDNA. The predicted precursor polypeptide is 488 amino acids long, including the 22 amino acids of the putative signal sequence. The amino acid composition of cruciferin protein is very similar to the predicted composition of the precursor. Comparison with an amino acid sequence of legumin from peas, deduced from the nucleotide sequence of a genomic clone, shows that the α polypeptide precedes the β polypeptide on the precursor. Cruciferin and legumin share 40% homology in the regions which can be aligned. However, cruciferin contains a 38 amino acid region high in glutamine and glycine in the middle of the α subunit, which is absent in legumin. Legumin has a highly charged region, 57 amino acids long, at the carboxyl-end of the α subunit, which is not found in cruciferin. Both of these regions appear to have originated by reiteration of sequences. re]19850513 ac]19850715.
authors.affiliation: Department of Biology, Indiana University, 47405, Bloomington, IN, U.S.A..
authors.forename: A E
authors.initials: AE
authors.lastname: Simon
authors.forename: K M
authors.initials: KM
authors.lastname: Tenbarge
authors.forename: S R
authors.initials: SR
authors.lastname: Scofield
authors.forename: R R
authors.initials: RR
authors.lastname: Finkelstein
authors.forename: M L
authors.initials: ML
authors.lastname: Crouch