title: Fully automated compound screening in Arabidopsis thaliana seedlings.
abstract: High-throughput small molecule screenings in model plants are of great value to identify compounds that interfere with plant developmental processes. In academic research, the plant Arabidopsis thaliana is the most commonly used model organism for this purpose. However, compared to plant cellular systems, A. thaliana plants are less amenable to develop high-throughput screening assays. In this chapter, we describe a screening procedure that is compatible with liquid handling systems and increases the throughput of compound screenings in A. thaliana seedlings.
authors.affiliation: Department of Plant Systems Biology, VIB, Gent, Belgium.
authors.forename: Dominique
authors.initials: D
authors.lastname: Audenaert
authors.forename: Long
authors.initials: L
authors.lastname: Nguyen
authors.forename: Bert
authors.initials: B
authors.lastname: De Rybel
authors.forename: Tom
authors.initials: T
authors.lastname: Beeckman
mesh_terms: D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D057205:Automation, Laboratory; D015870:Gene Expression / Q000187:drug effects; D017930:Genes, Reporter; D057166:High-Throughput Screening Assays; D010937:Plant Growth Regulators / Q000494:pharmacology*; D030821:Plants, Genetically Modified / Q000187:drug effects; D036226:Seedlings / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D010937:Plant Growth Regulators