title: Genetic diversity of ORF3 and spike genes of porcine epidemic diarrhea virus in Thailand.
abstract: Porcine epidemic diarrhea virus (PEDV) has become endemic in the Thai swine industry, causing economic losses and repeated outbreaks since its first emergence in 2007. In the present study, 69 Thai PEDV isolates were obtained from 50 swine herds across Thailand during the period 2008-2012. Both partial and complete nucleotide sequences of the spike (S) glycoprotein and the nucleotide sequences of ORF3 genes were determined to investigate the genetic diversity and molecular epidemiology of Thai PEDV. Based on the analysis of the partial S glycoprotein genes, the Thai PEDV isolates were clustered into 2 groups related to Korean and Chinese field isolates. The results for the complete spike genes, however, demonstrated that both groups were grouped in the same cluster. Interestingly, both groups of Thai PEDV isolates had a 4-aa (GENQ) insertion between positions 55 and 56, a 1-aa insertion between positions 135 and 136, and a 2-aa deletion between positions 155 and 156, making them identical to the Korean KNU series and isolates responsible for outbreaks in China in recent years. In addition to the complete S sequences, the ORF3 gene analyses suggested that the isolates responsible for outbreaks in Thailand are not vaccine related. The results of this study suggest that the PEDV isolates responsible for outbreaks in Thailand since its emergence represent a variant of PEDV that was previously reported in China and Korea.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Veterinary Science, Bangkok 10330, Thailand.
authors.forename: Gun
authors.initials: G
authors.lastname: Temeeyasen
authors.affiliation: Department of Veterinary Microbiology, Faculty of Veterinary Science, Bangkok 10330, Thailand.
authors.forename: Anchalee
authors.initials: A
authors.lastname: Srijangwad
authors.affiliation: Department of Veterinary Microbiology, Faculty of Veterinary Science, Bangkok 10330, Thailand.
authors.forename: Thitima
authors.initials: T
authors.lastname: Tripipat
authors.affiliation: Genome Institute, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand.
authors.forename: Pavita
authors.initials: P
authors.lastname: Tipsombatboon
authors.affiliation: Genome Institute, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand.
authors.forename: Jittima
authors.initials: J
authors.lastname: Piriyapongsa
authors.affiliation: Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Waranyoo
authors.initials: W
authors.lastname: Phoolcharoen
authors.affiliation: Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Taksina
authors.initials: T
authors.lastname: Chuanasa
authors.affiliation: Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Angkana
authors.initials: A
authors.lastname: Tantituvanont
authors.affiliation: Department of Veterinary Microbiology, Faculty of Veterinary Science, Bangkok 10330, Thailand. Electronic address: dachrit@gmail.com.
authors.forename: Dachrit
authors.initials: D
authors.lastname: Nilubol
mesh_terms: D000818:Animals; D018352:Coronavirus Infections / Q000453:epidemiology / Q000662:veterinary*; D019143:Evolution, Molecular; D014644:Genetic Variation; D016366:Open Reading Frames; D010802:Phylogeny; D053485:Porcine epidemic diarrhea virus / Q000145:classification* / Q000235:genetics*; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*; D013785:Thailand / Q000453:epidemiology; D014764:Viral Proteins / Q000235:genetics*; D014765:Viral Vaccines / Q000235:genetics
chemical_list: D014764:Viral Proteins; D014765:Viral Vaccines
keywords: Diversity; ORF3 gene; Porcine epidemic diarrhea virus; Spike gene; Thailand