title: Discrimination between 5-hydroxymethylcytosine and 5-methylcytosine in DNA by selective chemical labeling.
abstract: Detection of DNA damage has been greatly improved following the development of equipment and techniques, however, discrimination between 5-hydroxymethylcytosine (5-hmC) and 5-methylcytosine (5-mC) is still a thorny problem. In the present study, an approach to oxidize and selective label (Ox-Labeling) 5-hmC in native DNA has been reported, which conveniently distinguishes 5-hmC from 5-mC using simple and effective processes.
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, PR China.
authors.forename: Jianlin
authors.initials: J
authors.lastname: Hu
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, PR China.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, PR China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Xu
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Wu
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, PR China.
authors.forename: Xiwen
authors.initials: X
authors.lastname: Xing
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, PR China.
authors.forename: Zhenghao
authors.initials: Z
authors.lastname: Xu
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, PR China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Xu
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, PR China.
authors.forename: Xiaocheng
authors.initials: X
authors.lastname: Weng
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, PR China; State Key Laboratory of Natural and Biomimetic Drugs, Peking University, PR China. Electronic address: xzhou@whu.edu.cn.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
mesh_terms: D044503:5-Methylcytosine / Q000737:chemistry* / Q000031:analogs & derivatives; D018360:Crystallography, X-Ray; D003596:Cytosine / Q000031:analogs & derivatives* / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure
chemical_list: C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA
keywords: Chemoselectivity; DNA damage; Fluorescent dyes; Nucleosides; Oligodeoxyribonucleotides