title: Investigation of targeted biomolecules in a micro-fluxgate-based bio-sensing system.
abstract: An investigation of targeted biomolecules was accomplished by combining a micro-fluxgate-based bio-sensing system and Dynabeads. The fluxgate sensor for biomolecule detection was fabricated by Micro Electro-Mechanical system technology, including thick photoresist lithography, electroplating and chemical wet etching. The magnetic core of the sensor was made of Fe-based amorphous ribbon core and three dimension solenoid coils were used as magnetic sensitive elements. The micro-fluxgate-based bio-sensing system was characterized firstly in different concentrations of Dynabeads, and a concentration as low as 100 ng/ml was detected with an external dc magnetic field in the range of 525 μT to 875 μT. Sandwich assays are performed using antibody-antigen pair combination of biotin-streptavidin on a separated Au film substrate surface with a self-assembled layer. Detection of Alpha Fetoprotein antigens with different concentrations was performed and a minimum detectable concentration of 1 pg/ml was achieved by the bio-sensing system. It is of considerable interest due to its potential application in the biomedical field based on known specific binding of target and labels.
authors.affiliation: Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Research Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, Dongchuan Road 800, Shanghai, 200240, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Lei
authors.forename: Chong
authors.initials: C
authors.lastname: Lei
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008567:Membranes, Artificial; D012680:Sensitivity and Specificity; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D008567:Membranes, Artificial; D000509:alpha-Fetoproteins; D006046:Gold