title: Schiff base metal derivatives enhance the expression of HSP70 and suppress BAX proteins in prevention of acute gastric lesion.
abstract: Schiff base complexes have appeared to be promising in the treatment of different diseases and disorders and have drawn a lot of attention to their biological activities. This study was conducted to evaluate the regulatory effect of Schiff base metal derivatives on the expression of heat shock proteins (HSP) 70 and BAX in protection against acute haemorrhagic gastric ulcer in rats. Rats were assigned to 6 groups of 6 rats: the normal control (Tween 20 5% v/v, 5 mL/kg), the positive control (Tween 20 5% v/v, 5 mL/kg), and four Schiff base derivative groups named Schiff_1, Schiff_2, Schiff_3, and Schiff_4 (25 mg/kg). After 1 h, all of the groups received ethanol 95% (5 mL/kg) but the normal control received Tween 20 (Tween 20 5% v/v, 5 mL/kg). The animals were euthanized after 60 min and the stomachs were dissected for histology (H&E), immunohistochemistry, and western blot analysis against HSP70 and BAX proteins. The results showed that the Schiff base metal derivatives enhanced the expression of HSP70 and suppressed the expression of BAX proteins during their gastroprotection against ethanol-induced gastric lesion in rats.
authors.affiliation: Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Shahram
authors.initials: S
authors.lastname: Golbabapour
authors.forename: Nura Suleiman
authors.initials: NS
authors.lastname: Gwaram
authors.forename: Mazen M Jamil
authors.initials: MM
authors.lastname: Al-Obaidi
authors.forename: A F
authors.initials: AF
authors.lastname: Soleimani
authors.forename: Hapipah Mohd
authors.initials: HM
authors.lastname: Ali
authors.forename: Nazia
authors.initials: N
authors.lastname: Abdul Majid
mesh_terms: D000208:Acute Disease; D000818:Animals; D002492:Central Nervous System Depressants / Q000009:adverse effects / Q000494:pharmacology; D000431:Ethanol / Q000009:adverse effects / Q000494:pharmacology; D006471:Gastrointestinal Hemorrhage / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D005786:Gene Expression Regulation / Q000187:drug effects*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D008670:Metals / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012545:Schiff Bases / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D013276:Stomach Ulcer / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis*
chemical_list: C494828:Bax protein, rat; D002492:Central Nervous System Depressants; D018840:HSP70 Heat-Shock Proteins; D008670:Metals; D012545:Schiff Bases; D051028:bcl-2-Associated X Protein; D000431:Ethanol