title: Genome-wide expressional and functional analysis of calcium transport elements during abiotic stress and development in rice.
abstract: Ca²⁺ homeostasis is required to maintain a delicate balance of cytosolic Ca²⁺ during normal and adverse growth conditions. Various Ca²⁺ transporters actively participate to maintain this delicate balance especially during abiotic stresses and developmental events in plants. In this study, we present a genome-wide account, detailing expression profiles, subcellular localization and functional analysis of rice Ca²⁺ transport elements. Exhaustive in silico data mining and analysis resulted in the identification of 81 Ca²⁺ transport element genes, which belong to various groups such as Ca²⁺-ATPases (pumps), exchangers, channels, glutamate receptor homologs and annexins. Phylogenetic analysis revealed that different Ca²⁺ transporters are evolutionarily conserved across different plant species. Comprehensive expression analysis by gene chip microarray and quantitative RT-PCR revealed that a substantial proportion of Ca²⁺ transporter genes were expressed differentially under abiotic stresses (salt, cold and drought) and reproductive developmental stages (panicle and seed) in rice. These findings suggest a possible role of rice Ca²⁺ transporters in abiotic stress and development triggered signaling pathways. Subcellular localization of Ca²⁺ transporters from different groups in Nicotiana benthamiana revealed their variable localization to different compartments, which could be their possible sites of action. Complementation of Ca²⁺ transport activity of K616 yeast mutant by Ca²⁺-ATPase OsACA7 and involvement in salt tolerance verified its functional behavior. This study will encourage detailed characterization of potential candidate Ca²⁺ transporters for their functional role in planta.
authors.affiliation: Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi-110021, India.
authors.forename: Amarjeet
authors.initials: A
authors.lastname: Singh
authors.forename: Poonam
authors.initials: P
authors.lastname: Kanwar
authors.forename: Akhilesh K
authors.initials: AK
authors.lastname: Yadav
authors.forename: Manali
authors.initials: M
authors.lastname: Mishra
authors.forename: Saroj K
authors.initials: SK
authors.lastname: Jha
authors.forename: Vinay
authors.initials: V
authors.lastname: Baranwal
authors.forename: Amita
authors.initials: A
authors.lastname: Pandey
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kapoor
authors.forename: Akhilesh K
authors.initials: AK
authors.lastname: Tyagi
authors.forename: Girdhar K
authors.initials: GK
authors.lastname: Pandey
mesh_terms: D052636:Allostasis / Q000235:genetics*; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003080:Cold Temperature; D055864:Droughts; D020440:Gene Duplication; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D055106:Genome-Wide Association Study; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D056626:Inflorescence / Q000254:growth & development / Q000378:metabolism; D012275:Oryza / Q000254:growth & development / Q000502:physiology*; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D054712:Salinity; D012639:Seeds / Q000254:growth & development / Q000378:metabolism; D013312:Stress, Physiological; D014026:Nicotiana / Q000166:cytology / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D000252:Calcium-Transporting ATPases; D002118:Calcium
keywords: Ca2+ transporter; abiotic stress; development; expression; signal transduction