title: Cytotoxic and apoptotic effects of different extracts of Artemisia turanica Krasch. on K562 and HL-60 cell lines.
abstract: Artemisia is an important genus of Iranian flora. Cytotoxic activities for some species of the genus have already been reported. In this study, we have investigated the cytotoxic effects of n-hexane, CH2Cl2, EtOAc, EtOH, and EtOH/H2O (1:1) extracts of A. turanica Krasch. on two human leukemic cancer cell lines (K562 and HL-60) and J774 as normal cells using alamarBlue (resazurin) assay. PI staining of the fragmented DNA and western blot analysis were used to evaluate the possible apoptotic effect of the extract. The CH2Cl2 extract of A. turanica showed the most antiproliferative effect on cancer cells among all tested extracts with IC50 values of 69 and 104 μ g/mL on K562 and HL-60 cells, respectively, whereas the normal cells were not affected significantly by this extract. Sub-G1 peak in the flow cytometry histogram of the cells treated with CH2Cl2 extract of A. turanica and cleavage of PARP protein confirmed the induction of apoptosis with CH2Cl2 extract. Taken together, the findings of the present work suggest the anticancer potential of CH2Cl2 extract of A. turanica on human leukemic cancer cell lines.
authors.affiliation: Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad 9188617871, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Tayarani-Najaran
authors.forename: Mahla
authors.initials: M
authors.lastname: Sareban
authors.forename: Atefeh
authors.initials: A
authors.lastname: Gholami
authors.forename: Seyed Ahmad
authors.initials: SA
authors.lastname: Emami
authors.forename: Mahdi
authors.initials: M
authors.lastname: Mojarrab
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D019306:Artemisia / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D010936:Plant Extracts / Q000633:toxicity*
chemical_list: D010936:Plant Extracts