title: Enzymes inhibition and antidiabetic effect of isolated constituents from Dillenia indica.
abstract: OBJECTIVE
  This study was designed to investigate the enzyme inhibitory and antidiabetic activity for the constituents isolated from Dillenia indica.
  
  
  METHODS
  The leaves of D. indica were extracted with methanol and subjected to fractionation and chromatographic separation, which led to the isolation of seven compounds: betulinic acid (1), n-heptacosan-7-one (2), n-nonatriacontan-18-one (3), quercetin (4), β sitosterol (5), stigmasterol (6), and stigmasteryl palmitate (7). Among these isolates, compounds 1, 4, 5, and 6 were evaluated for in vitro enzyme inhibition and compounds 4, 5 and 6 were evaluated for antidiabetic activity in streptozotocin-nicotinamide induced diabetic mice.
  
  
  RESULTS
  Compounds 1, 4, 5, and 6 showed 47.4, 55.2, 48.8, and 44.3% α -amylase inhibition, respectively, and 52.2, 78.2, 52.5, and 34.2% α -glucosidase inhibition, respectively, at the dose of 50 µg/kg. Compounds 4, 5 and 6 also showed significant (∗P < 0.05) antidiabetic activity in streptozotocin-nicotinamide induced diabetic mice at the dose of 10 mg/kg.
  
  
  CONCLUSIONS
  These results provide evidence that Dillenia indica might be a potential source of antidiabetic agents.
authors.affiliation: Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra, Haryana 136119, India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Kumar
authors.forename: Vipin
authors.initials: V
authors.lastname: Kumar
authors.forename: Om
authors.initials: O
authors.lastname: Prakash
mesh_terms: D000681:Amylases / Q000037:antagonists & inhibitors; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000473:pathology; D045466:Dilleniaceae / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D001786:Blood Glucose; D004791:Enzyme Inhibitors; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D000681:Amylases