title: Signal and depth enhancement for in vivo flow cytometer measurement of ear skin by optical clearing agents.
abstract: The in vivo flow cytometry (IVFC) has shown a great potential for detecting circulating tumor cells quantitatively in the bloodstream. However, the detection depth suffers from the strong light scattering of tissue. In this study, an innovative ear skin optical clearing agent (ESOCA) is employed to improve the signal quality of the IVFC. Our results show that compared with commonly used glycerol, topical application of ESOCA can enhance the transmittance of rat ear significantly in vivo. The labeled red blood cells can be detected by the IVFC with higher signal quality and greater detection depth. This study is very helpful for potential tumor metastasis studies by the IVFC in deep tissues.
authors.affiliation: State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, China ; These authors contributed equally to this work.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Ding
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Fan
authors.forename: Dan
authors.initials: D
authors.lastname: Wei
authors.forename: Rui
authors.initials: R
authors.lastname: Shi
authors.forename: Qingming
authors.initials: Q
authors.lastname: Luo
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
authors.forename: Xunbin
authors.initials: X
authors.lastname: Wei
keywords: (170.1470) Blood or tissue constituent monitoring; (170.1530) Cell analysis; (170.3660) Light propagation in tissues