title: Characterization of photosystem II mutants of Chlamydomonas reinhardii lacking the psbA gene.
abstract: We have examined 78 chloroplast mutants of Chlamydomonas reinhardii lacking photosystem II activity. Most of them are unable to synthesize the 32 Kdalton protein. Analysis of 22 of these mutants reveals that they have deleted both copies of the psbA gene (which codes for the 32 Kdalton protein) in their chloroplast genome. Although these mutants are able to synthesize and to integrate the other photosystem II polypeptides in the thylakoid membranes, they are unable to assemble a stable functional photosystem II complex. The 32 Kprotein appears therefore to play an important role not only in photosystem II function, but also in stabilizing this complex.
authors.affiliation: Institut de Biologie Physico-chimique, 75005, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Bennoun
authors.forename: M
authors.initials: M
authors.lastname: Spierer-Herz
authors.forename: J
authors.initials: J
authors.lastname: Erickson
authors.forename: J
authors.initials: J
authors.lastname: Girard-Bascou
authors.forename: Y
authors.initials: Y
authors.lastname: Pierre
authors.forename: M
authors.initials: M
authors.lastname: Delosme
authors.forename: J D
authors.initials: JD
authors.lastname: Rochaix