title: Early-effect of bariatric surgery (Scopinaro method) on intestinal hormones and adipokines in insulin resistant Wistar rat.
abstract: Bariatric surgery consists in duodenal exclusion from the food passage in obese patients with coexistent type 2 diabetes. Nowadays bariatric surgery is considered the most effective method of glycemic index normalization and insulin resistance reduction. Recent results on obese and non-obese rats showed remission of type 2 diabetes symptoms within few days after the surgery. The aim of the present work was to analyze the mechanisms of neuro-hormonal regulation responsible for early normalization of metabolic syndrome after bariatric surgery. In present study the concentration of selected intestinal hormones and adipokines in blood plasma and gastrointestinal tissues were analyzed. Study was conducted on Wistar rats. Animals were divided into three groups (each n=6): control (SH) shame-operated rats; animals in which visceral fat tissue was extracted (LP); and rats in which Scopinaro bariatric surgery was performed (BPD). Immunochemistry analysis of blood plasma showed decrease of insulin concentration in BPD and LP and increase of polypeptide YY (PYY) in BPD group as compared to the control. In duodenal mucosa homogenates the tendency to reduce insulin in LP and BPD group, and increase PYY and visfatin in BPD group was observed. Histometry analysis showed reduction of mucosa thickness in excluded segments of gastrointestinal tract in BPD group as compared to the SH and LP. Concluding, model studies on rats allowed better understanding of mechanisms important for early normalization of glycemic index and insulin resistance reduction in rats.
authors.affiliation: Department of General, Oncological and Gastrointestinal Surgery, Orlowski Hospital MCPE, Warsaw, Poland. rzabielski@plusnet.pl.
authors.forename: N
authors.initials: N
authors.lastname: Dib
authors.forename: A
authors.initials: A
authors.lastname: Kiciak
authors.forename: P
authors.initials: P
authors.lastname: Pietrzak
authors.forename: K
authors.initials: K
authors.lastname: Ferenc
authors.forename: P
authors.initials: P
authors.lastname: Jaworski
authors.forename: M
authors.initials: M
authors.lastname: Kapica
authors.forename: W
authors.initials: W
authors.lastname: Tarnowski
authors.forename: R
authors.initials: R
authors.lastname: Zabielski
mesh_terms: D054392:Adipokines / Q000378:metabolism; D000818:Animals; D000073861:Apelin; D050110:Bariatric Surgery; D016207:Cytokines / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism; D007333:Insulin Resistance / Q000502:physiology*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism; D008297:Male; D054409:Nicotinamide Phosphoribosyltransferase / Q000378:metabolism*; D019894:Peptide YY / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D054392:Adipokines; D000073861:Apelin; C498579:Apln protein, rat; D016207:Cytokines; D007328:Insulin; D036341:Intercellular Signaling Peptides and Proteins; D019894:Peptide YY; D054409:Nicotinamide Phosphoribosyltransferase; C504970:nicotinamide phosphoribosyltransferase, rat