title: Inorganic/organic doped carbon aerogels as biosensing materials for the detection of hydrogen peroxide.
abstract: In this article, three different inorganic/organic doped carbon aerogel (CA) materials (Ni-CA, Pd-CA, and Ppy-CA) were, respectively, mixed with ionic liquid (IL) to form three stable composite films, which were used as enhanced elements for an integrated sensing platform to increase the surface area and to improve the electronic transmission rate. Subsequently, the effect of the materials performances such as adsorption, specific surface area and conductivity on electrochemistry for myoglobin (Mb) was discussed using N2 adsorption-desorption isotherm measurements, scanning electron microscopy (SEM), and electrochemical impedance spectroscopy (EIS). Moreover, they could act as sensors toward the detection of hydrogen peroxide (H2O2) with lower detection limits (1.68 μM, 1.02 μM, and 0.85 μM, for Ni-CA/IL/Mb-CPE, Pd-CA/IL/Mb-CPE, and Ppy-CA/IL/Mb-CPE, respectively) and smaller apparent Michaelis-Menten constants KM. The results indicated that the electroconductibility of the doped CA materials would become dominant, thus playing an important role in facilitating the electron transfer. Meanwhile, the synergetic effect with [BMIm]BF4 IL improved the capability of the composite inorganic/organic doped CA/IL matrix for protein immobilization. This work demonstrates the feasibility and the potential of a series of CA-based hybrid materials as biosensors, and further research and development are required to prepare other functional CAs and make them valuable for more extensive application in biosensing.
authors.affiliation: College of Sciences, Xi'an University of Architecture and Technology , Xi'an 710055, People's Republic of China.
authors.forename: Sheying
authors.initials: S
authors.lastname: Dong
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Gaochao
authors.initials: G
authors.lastname: Suo
authors.forename: Tinglin
authors.initials: T
authors.lastname: Huang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D002417:Cattle; D004563:Electrochemistry; D004566:Electrodes; D005782:Gels; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D009211:Myoglobin / Q000737:chemistry; D009532:Nickel / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D005782:Gels; D009211:Myoglobin; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D010165:Palladium; D002244:Carbon; D009532:Nickel; D006861:Hydrogen Peroxide