title: [Genetic polymorphisms of 15 X-STR loci in Shandong Han population].
abstract: OBJECTIVE
  To investigate the genetic polymorphisms of 15 X-STR loci in Shandong Han population in order to establish the forensic application database.
  
  
  METHODS
  The multi-PCR primers of these loci were designed by Primer Premier 5.0 software and labeled by 4 fluoresceins (FAM, VIC, NED and TET). The developed multi-PCR was used to investigate 15 X-STR loci (DXS10011, DXS101, GATA 165B12, DXS6795, DXS6800, DXS6801, DXS6803, DXS7132, DXS7133, DXS7423, DXS7424, DXS8377, DXS8378, DXS9898 and HPRTB) selected from the X chromosome of 481 unrelated individuals (295 females and 186 males) in Shandong Han population.
  
  
  RESULTS
  Among the 15 X-STR loci, GATA 165B12, DXS6800, DXS6803, DXS7133 and DXS7423 showed moderate polymorphisms, while the rest 10 X-STR loci showed high polymorphisms (PIC > 0.5 and H > 0.5). No shared haplotype was detected among the males in Shandong Han population.
  
  
  CONCLUSIONS
  The developed multi-PCR system with fluorescence detection provides an effective way to establish X-STR loci database of population genetics in Shandong Han population and shows its forensic application.
authors.affiliation: Ministry of Education Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai 200433, China. mt152630@163.com
authors.forename: Teng
authors.initials: T
authors.lastname: Ma
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Yang
authors.forename: Kuan
authors.initials: K
authors.lastname: Sun
authors.forename: Fu-Zhong
authors.initials: FZ
authors.lastname: Xue
authors.forename: Li
authors.initials: L
authors.lastname: Jin
authors.forename: Shi-Lin
authors.initials: SL
authors.lastname: Li
mesh_terms: D044466:Asian People / Q000208:ethnology / Q000235:genetics*; D002681:China; D041321:Chromosomes, Human, X / Q000235:genetics*; D016172:DNA Fingerprinting; D017931:DNA Primers; D005260:Female; D053803:Forensic Genetics; D005787:Gene Frequency; D008040:Genetic Linkage; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: D017931:DNA Primers