title: In situ forming antibacterial dextran blend hydrogel for wound dressing: SAA technology vs. spray drying.
abstract: This study focuses on designing microparticulate carriers based on high-mannuronic alginate and amidated pectin blend loaded with gentamicin sulphate able to move rapidly from dry to soft hydrogel. Supercritical assisted atomization was used to produce microparticles in form of dry powder and characteristics were compared with those obtained by spray-drying. Particles with very high encapsulation efficiency (approximately 100%) and small diameter (less than 2 μm) showed good flowability and high fluid uptake enabling wound site filling and limiting bacterial proliferation. Moisture transmission of the in situ formed hydrogel was about 95 g/m(2)h, ideal to avoid wound dehydration or occlusion phenomena. All formulations presented a burst effect, suitable to prevent infection spreading at the beginning of the therapy, followed by prolonged release (4-10 days) related to drug/polymers ratio. Antimicrobial tests showed stronger effect than pure GS over time (up-to 24 days) and the ability to degrade preformed biofilms, essential to properly treat infected wounds.
authors.affiliation: Department of Pharmacy, University of Salerno, via Giovanni Paolo II, 132, 84084 Fisciano SA, Italy.
authors.forename: Felicetta
authors.initials: F
authors.lastname: De Cicco
authors.forename: Ernesto
authors.initials: E
authors.lastname: Reverchon
authors.forename: Renata
authors.initials: R
authors.lastname: Adami
authors.forename: Giulia
authors.initials: G
authors.lastname: Auriemma
authors.forename: Paola
authors.initials: P
authors.lastname: Russo
authors.forename: Elena C
authors.initials: EC
authors.lastname: Calabrese
authors.forename: Amalia
authors.initials: A
authors.lastname: Porta
authors.forename: Rita P
authors.initials: RP
authors.lastname: Aquino
authors.forename: Pasquale
authors.initials: P
authors.lastname: Del Gaudio
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D001458:Bandages / Q000382:microbiology*; D003911:Dextrans / Q000737:chemistry* / Q000494:pharmacology*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D010539:Permeability; D013211:Staphylococcus aureus / Q000187:drug effects; D014945:Wound Healing
chemical_list: D000900:Anti-Bacterial Agents; D003911:Dextrans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
keywords: Alginate-pectin blend; Antimicrobial activity; In situ dextran gelling formulation; Supercritical assisted atomization; Topical controlled release; Wound dressing