title: Assessment of inhibitory potential of Pothos scandens L. on ovalbumin-induced airway hyperresponsiveness in balb/c mice.
abstract: Pothos scandens L. was used in Indian traditional medicine as an antiasthmatic drug. The ethanolic and aqueous extracts were prepared with aerial parts of P. scandens (PSE & PSA). ESI MS/MS of PSE ethanolic extract was carried out for the determination of chemical constituents. CP1 is isolated from the PSE, structurally confirmed with NMR and LCMS/MS. PSE, PSA and CP1 are evaluated against ovalbumin (OVA) induced airway hyperresponsiveness (AHR) in balb/c mice. The test drugs are administered p.o. prior to challenge with aerosolized 2.5% w/v OVA. Total and differential leucocyte count, nitrite (NO2), nitrate (NO3), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-13 (IL-13) are estimated in bronchoalveolar lavage fluid (BALF). Similarly, myeloperoxidase (MPO), malonaldehyde (MDA) and total lung protein (TLP) are estimated in the lungs. The results reveal a significant increase in total and differential leucocyte count, NO2, NO3, TNF-α, IL-6, and IL-13 in OVA induced AHR. However, these parameters are significantly decreased in PSE and PSA tested doses (PSE 100 & 200mg/kg). While, treatment with CP1 is less effective at 5 & 10mg/kg doses. Similar observations obtain for MPO and MDA in lungs. However, the mean value indicated that the PSE at 200mg/kg showed a significant restoration in all the parameters. Pro-inflammatory mediators are known to be responsible for AHR. Histopathology revealed justifies the effectiveness. The present investigations suggest PSE are interesting molecules for further research for asthma, with an approach through pro-inflammatory inhibitory pathway. P. scandens is a potential herbal medicine for allergy induced asthma.
authors.affiliation: Department of Pharmacognosy, J.S.S. College of Pharmacy (Off Campus JSS University), Ootacamund 643 001, TN, India. Electronic address: saurabhgupta80@gmail.com.
authors.forename: Saurabh
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Department of Pharmacognosy, J.S.S. College of Pharmacy (Off Campus JSS University), Ootacamund 643 001, TN, India.
authors.forename: Duraiswamy
authors.initials: D
authors.lastname: Basavan
authors.affiliation: Department of Pharmacology, J.S.S. College of Pharmacy (Off Campus JSS University), Ootacamund 643 001, TN, India.
authors.forename: Satish Kumar
authors.initials: SK
authors.lastname: Muthureddy Nataraj
authors.affiliation: Department of Pharmacology, J.S.S. College of Pharmacy (Off Campus JSS University), Ootacamund 643 001, TN, India.
authors.forename: K Rama Satyanarayana
authors.initials: KR
authors.lastname: Raju
authors.affiliation: The Himalaya Drug Company, Makali, Bangalore-562 123, Karnataka, India.
authors.forename: U V
authors.initials: UV
authors.lastname: Babu
authors.affiliation: The Himalaya Drug Company, Makali, Bangalore-562 123, Karnataka, India.
authors.forename: Sharath Kumar
authors.initials: SK
authors.lastname: L M
authors.affiliation: Dr. Batra's Clinic, Nirala Bazaar, Aurangabad 431 001, Maharashtra, India.
authors.forename: Renu
authors.initials: R
authors.lastname: Gupta
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D029064:Araceae; D016535:Bronchial Hyperreactivity / Q000188:drug therapy* / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D007962:Leukocytes / Q000187:drug effects* / Q000276:immunology; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008519:Medicine, Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D009566:Nitrates / Q000378:metabolism; D010047:Ovalbumin / Q000276:immunology; D009195:Peroxidase / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage
chemical_list: D000485:Allergens; D016207:Cytokines; D018836:Inflammation Mediators; D009566:Nitrates; D010936:Plant Extracts; D008315:Malondialdehyde; D010047:Ovalbumin; D009195:Peroxidase
keywords: Anti asthmatic; Interlukin-13 (IL-13); Interlukin-6 (IL-6); Pothos scandens; Tumor necrosis factor-alpha (TNF-α)