title: Targeting microRNA-23a to inhibit glioma cell invasion via HOXD10.
abstract: Glioma is the most frequent primary brain tumor. Recently, the upregulation of microRNA (miR)-23a was found to be associated with glioma, but the molecular mechanism by which miR-23a promotes glioma growth remains to be unveiled. In the present study, we found that miR-23a was significantly upregulated in glioma tissues compared to their matched adjacent tissues. miR-23a was also highly expressed in glioma cell lines SHG44, U251, and U87 cells. Moreover, we identified homeobox D10 (HOXD10) as a novel target for miR-23a. The expression of HOXD10 was significantly reduced in glioma tissues and cell lines, and miR-23a negatively regulates the protein expression of HOXD10 in U251 and U87 cells. We further showed that miRNA-23a promoted U251 and U87 cell invasion, at least partially, by directly targeting HOXD10 and further modulating MMP-14. These findings suggest that miR-23a may serve as a promising therapeutic target for glioma.
authors.affiliation: Department of Anatomy and Neurobiology, School of Basic Medical Sciences, Central South University, Changsha, Hunan Province, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Hu
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Cui
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Li
authors.forename: Jufang
authors.initials: J
authors.lastname: Huang
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D018398:Homeodomain Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D018398:Homeodomain Proteins; C552430:MIRN23a microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D014157:Transcription Factors; C072064:HOXD10 protein, human