title: Enzyme-free fluorescent-amplified aptasensors based on target-responsive DNA strand displacement via toehold-mediated click chemical ligation.
abstract: A new target-responsive DNA strand displacement system via toehold-mediated click chemical ligation was designed and prepared for enzyme-free fluorescent-amplified aptasensors. The aptasensors significantly amplified fluorescent signals in response to targets based on target recycling processes.
authors.affiliation: Division of Materials Science, Faculty of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8573, Japan. oishi@ims.tsukuba.ac.jp.
authors.forename: Motoi
authors.initials: M
authors.lastname: Oishi
authors.forename: Shingo
authors.initials: S
authors.lastname: Nakao
authors.forename: Daiki
authors.initials: D
authors.lastname: Kato
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D057930:Click Chemistry; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D009693:Nucleic Acid Hybridization
chemical_list: D052157:Aptamers, Nucleotide; D005452:Fluoresceins; D005456:Fluorescent Dyes; C081676:thrombin aptamer; C024098:6-carboxyfluorescein; D004247:DNA