title: Lanthanide(III) complexes of rhodamine-DO3A conjugates as agents for dual-modal imaging.
abstract: Two novel dual-modal MRI/optical probes based on a rhodamine-DO3A conjugate have been prepared. The bis(aqua)gadolinium(III) complex Gd.L1 and mono(aqua)gadolinium(III) complex Gd.L2 behave as dual-modal imaging probes (r1 = 8.5 and 3.8 mM(-1) s(-1) for Gd.L1 and Gd.L2, respectively; λex = 560 nm and λem = 580 nm for both complexes). The rhodamine fragment is pH-sensitive, and upon lowering of the pH, an increase in fluorescence intensity is observed as the spirolactam ring opens to give the highly fluorescent form of the molecule. The ligands are bimodal when coordinated to Tb(III) ions, inducing fluorescence from both the lanthanide center and the rhodamine fluorophore, on two independent time frames. Confocal imaging experiments were carried out to establish the localization of Gd.L2 in HEK293 cells and primary mouse islet cells (∼70% insulin-containing β cells). Colocalization with MitoTracker Green demonstrated Gd.L2's ability to distinguish between tumor and healthy cells, with compartmentalization believed to be in the mitochondria. Gd.L2 was also evaluated as an MRI probe for imaging of tumors in BALB/c nude mice bearing M21 xenografts. A 36.5% decrease in T1 within the tumor was observed 30 min post injection, showing that Gd.L2 is preferentially up taken in the tumor. Gd.L2 is the first small-molecule MR/fluorescent dual-modal imaging agent to display an off-on pH switch upon its preferential uptake within the more acidic microenvironment of tumor cells.
authors.affiliation: Department of Chemistry, Imperial College London , South Kensington, London SW7 2AZ, U.K.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Rivas
authors.forename: Graeme J
authors.initials: GJ
authors.lastname: Stasiuk
authors.forename: Juan
authors.initials: J
authors.lastname: Gallo
authors.forename: Florencia
authors.initials: F
authors.lastname: Minuzzi
authors.forename: Guy A
authors.initials: GA
authors.lastname: Rutter
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Long
mesh_terms: D000818:Animals; D002460:Cell Line; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D003952:Diagnostic Imaging; D005456:Fluorescent Dyes / Q000737:chemistry*; D005682:Gadolinium / Q000737:chemistry*; D006801:Humans; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D018613:Microscopy, Confocal; D015394:Molecular Structure; D009369:Neoplasms / Q000175:diagnosis; D012235:Rhodamines / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D005456:Fluorescent Dyes; D028581:Lanthanoid Series Elements; D012235:Rhodamines; D005682:Gadolinium