title: Infectious cDNA clones of four viroids in Coleus blumei and molecular characterization of their progeny.
abstract: Four viroid species infecting Coleus blumei, named Coleus blumei viroid 1-4 (CbVd-1-CbVd-4), and two tentative new viroid species, named CbVd-5 and CbVd-6, have been characterized, for two of which (CbVd-5 and CbVd-6, first reported in 2009), there is no established bioassay. Here, infectious clones were used as inoculums sources and the biological properties of CbVd-1, -3, -5 and -6 were assessed. When dimeric CbVd (+) RNAs synthesized in vitro were bioassayed, the first detection time for the four CbVds was different, ranging from 45 to 300 days. In addition, we confirmed that CbVd-5 and CbVd-6 can be transmitted via seeds. Molecular characterization of their progeny from slash-inoculated plants one year after inoculation demonstrated that the genetic diversity of CbVd populations may depend on the infected coleus plants and on the viroid genotype.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, PR China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Jiang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Gao
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
authors.forename: Lv
authors.initials: L
authors.lastname: Qin
authors.affiliation: Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, PR China.
authors.forename: Zujian
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, PR China.
authors.forename: Lianhui
authors.initials: L
authors.lastname: Xie
authors.affiliation: Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, PR China.
authors.forename: Wanying
authors.initials: W
authors.lastname: Hou
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China. Electronic address: sfli@ippcaas.cn.
authors.forename: Shifang
authors.initials: S
authors.lastname: Li
mesh_terms: D003001:Cloning, Molecular; D031330:Coleus / Q000821:virology*; D018076:DNA, Complementary; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification*; D014772:Viroids / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D014779:Virus Replication
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral
keywords: Coleus blumei; Host; Infectious clone; Viroid