title: Development of functional biointerfaces by surface modification of polydimethylsiloxane with bioactive chlorogenic acid.
abstract: The effect of physicochemical surface properties and chemical structure on the attachment and viability of bacteria and mammalian cells has been extensively studied for the development of biologically relevant applications. In this study, we report a new approach that uses chlorogenic acid (CA) to modify the surface wettability, anti-bacterial activity and cell adhesion properties of polydimethylsiloxane (PDMS). The chemical structure of the surface was obtained by X-ray photoelectron spectroscopy (XPS), the roughness was measured by atomic force microscopy (AFM), and the water contact angle was evaluated for PDMS substrates both before and after CA modification. Molecular modelling showed that the modification was predominately driven by van der Waals and electrostatic interactions. The exposed quinic-acid moiety improved the hydrophilicity of CA-modified PDMS substrates. The adhesion and viability of E. coli and HeLa cells were investigated using fluorescence and phase contrast microscopy. Few viable bacterial cells were found on CA-coated PDMS surfaces compared with unmodified PDMS surfaces. Moreover, HeLa cells exhibited enhanced adhesion and increased spreading on the modified PDMS surface. Thus, CA-coated PDMS surfaces reduced the ratio of viable bacterial cells and increased the adhesion of HeLa cells. These results contribute to the purposeful design of anti-bacterial surfaces for medical device use.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Science, Nankai University, Tianjin 300071, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wu
authors.affiliation: College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Jia
authors.initials: J
authors.lastname: He
authors.affiliation: Institute of Robotics and Automatic Information Systems, Nankai University, Tianjin 300071, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Ren
authors.affiliation: College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Wen-Sheng
authors.initials: WS
authors.lastname: Cai
authors.affiliation: Institute of Robotics and Automatic Information Systems, Nankai University, Tianjin 300071, China.
authors.forename: Yong-Chun
authors.initials: YC
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Science, Nankai University, Tianjin 300071, China. Electronic address: xzfeng@nankai.edu.cn.
authors.forename: Xi-Zeng
authors.initials: XZ
authors.lastname: Feng
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002726:Chlorogenic Acid / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D004926:Escherichia coli / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D013329:Structure-Activity Relationship; D013499:Surface Properties; D014407:Tumor Cells, Cultured; D017155:Wettability
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D004129:Dimethylpolysiloxanes; D002726:Chlorogenic Acid; C013830:baysilon
keywords: Anti-bacterial; Cell culture; Chlorogenic acid (CA); Molecular modelling; Polydimethylsiloxane (PDMS)