title: Calmodulin-like skin protein level increases in the differentiated epidermal layers in atopic dermatitis.
abstract: In atopic dermatitis (AD), the skin barrier is disturbed, and the expression of calcium-dependent S100 proteins and the calcium gradient is also altered in the epidermis. The calmodulin-like skin protein (CLSP), which is expressed in the differentiated epidermis, is believed to modulate the function of calcium-dependent proteins involved in barrier formation and is significantly increased in the epidermis of psoriatic patients. We, therefore, investigated the CLSP level in skin biopsies taken from patients with acute exacerbated and non-exacerbated AD as well as from healthy control subjects. Immunohistochemical, Western blot and ELISA analyses showed significant increases (P < 0.03) in CLSP level in the epidermis from patients with acute exacerbated AD as compared to that from patients with non-exacerbated AD and from control subjects. Such increased expression of CLSP may help re-establish a functional epidermal barrier in acute AD.
authors.affiliation: L'Oréal R & I, Aulnay sous Bois, France.
authors.forename: Mark
authors.initials: M
authors.lastname: Donovan
authors.forename: Andreas
authors.initials: A
authors.lastname: Ambach
authors.forename: Agnés
authors.initials: A
authors.lastname: Thomas-Collignon
authors.forename: Cecilia
authors.initials: C
authors.lastname: Prado
authors.forename: Dominique
authors.initials: D
authors.lastname: Bernard
authors.forename: Odette
authors.initials: O
authors.lastname: Jammayrac
authors.forename: Harald
authors.initials: H
authors.lastname: Gollnick
authors.forename: Olivier
authors.initials: O
authors.lastname: de Lacharriere
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D001706:Biopsy; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism; D016022:Case-Control Studies; D002454:Cell Differentiation; D003876:Dermatitis, Atopic / Q000378:metabolism*; D004817:Epidermis / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation; D015603:Keratinocytes / Q000166:cytology; D011565:Psoriasis / Q000378:metabolism; D009418:S100 Proteins / Q000378:metabolism; D014945:Wound Healing
chemical_list: D015415:Biomarkers; C408467:CALML5 protein, human; D002135:Calcium-Binding Proteins; D002147:Calmodulin; D009418:S100 Proteins; D002118:Calcium
keywords: atopic dermatitis; calcium; skin barrier; stratum corneum