title: Identification of the phosphoglycerate dehydrogenase isoform EDA9 as the essential gene for embryo and male gametophyte development in Arabidopsis.
abstract: Three different pathways of serine (Ser) biosynthesis have been described in plants: the Glycolate pathway, which is part of the Photorespiratory pathway, and 2 non-Photorespiratory pathways, the Glycerate and the Phosphorylated pathways. The Phosphorylated Pathway of Ser Biosynthesis (PPSB) has been known to exist since the 1950s, but its biological relevance was not revealed until quite recently when the last enzyme of the pathway, the Phosphoserine Phosphatase, was functionally characterized. In the associated study (1), we characterized a family of genes coding for putatite phosphoglycerate dehydrogenases (PGDH, 3-PGDH, and EDA9), the first enzyme of the PPSB. A metabolomics study using overexpressing plants indicated that all PGDH family genes were able to regulate Ser homeostasis but only lacking of EDA9 expression caused drastic developmental defects. We provided genetic and molecular evidence for the essential role of EDA9 for embryo and pollen development. Here, some new insights into the physiological/molecular function of PPSB and Ser are presented and discussed.
authors.affiliation: ERI de Biotecnologia i Biomedicina; Departament de Biologia Vegetal; Facultat de Farmàcia; Universitat de València; Valencia, Spain.
authors.forename: Walid
authors.initials: W
authors.lastname: Toujani
authors.affiliation: ERI de Biotecnologia i Biomedicina; Departament de Biologia Vegetal; Facultat de Farmàcia; Universitat de València; Valencia, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Muñoz-Bertomeu
authors.affiliation: ERI de Biotecnologia i Biomedicina; Departament de Biologia Vegetal; Facultat de Farmàcia; Universitat de València; Valencia, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Flores-Tornero
authors.affiliation: ERI de Biotecnologia i Biomedicina; Departament de Biologia Vegetal; Facultat de Farmàcia; Universitat de València; Valencia, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Rosa-Téllez
authors.affiliation: ERI de Biotecnologia i Biomedicina; Departament de Biologia Vegetal; Facultat de Farmàcia; Universitat de València; Valencia, Spain.
authors.forename: Armand
authors.initials: A
authors.lastname: Anoman
authors.affiliation: ERI de Biotecnologia i Biomedicina; Departament de Biologia Vegetal; Facultat de Farmàcia; Universitat de València; Valencia, Spain.
authors.forename: Roc
authors.initials: R
authors.lastname: Ros
mesh_terms: D017360:Arabidopsis / Q000196:embryology* / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D020043:Genes, Essential; D017343:Genes, Plant; D007527:Isoenzymes / Q000378:metabolism; D050543:Phosphoglycerate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011058:Pollen / Q000196:embryology* / Q000201:enzymology / Q000235:genetics; D012639:Seeds / Q000196:embryology* / Q000201:enzymology / Q000235:genetics; D012694:Serine / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D007527:Isoenzymes; D012694:Serine; D050543:Phosphoglycerate Dehydrogenase
keywords: embryo development; male gametophyte; phosphoglycerate dehydrogenase; phosphorylated pathway of serine biosynthesis