title: DNA-templated native chemical ligation of functionalized peptide nucleic acids: a versatile tool for single base-specific detection of nucleic acids.
abstract: Single base-specific detection of DNA/RNA sequences is of importance in the diagnosis of disease-associated genetic disorders or early stage cancer. This chapter introduces DNA-templated native chemical PNA ligation as a potentially useful tool for the sequence specific detection of nucleic acids. The template-induced alignment of PNA-thioesters and 1,2-aminothiol-PNAs in close proximity leads to an increase in their effective molarities. This facilitates PNA ligation to proceed at concentrations where no reaction would be possible in absence of the template. Moreover, hybridization of the rather short PNA conjugates with non-complementary DNA/RNA is disfavored, which prevents PNA ligation to occur on single base-mismatched templates. Different readout strategies of the ligation reaction such as HPLC, MALDI-TOF-MS and fluorecence monitoring are discussed, and examples for the detection of a point mutation within single stranded and PCR-amplified double stranded DNA are provided.
authors.affiliation: Department of Organic and Bioorganic Chemistry, Humboldt-Universität zu Berlin, Berlin, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Roloff
authors.forename: Simon
authors.initials: S
authors.lastname: Ficht
authors.forename: Christian
authors.initials: C
authors.lastname: Dose
authors.forename: Oliver
authors.initials: O
authors.lastname: Seitz
mesh_terms: D020137:Base Pair Mismatch; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature
chemical_list: D020135:Peptide Nucleic Acids; D004247:DNA