title: pH-upshock yields more lipids in nitrogen-starved Neochloris oleoabundans.
abstract: This study explores the influence of alkaline pH and light intensity on the performance of Neochloris oleoabundans in two-stage batch cultivation: a first stage for nitrogen-sufficient growth followed by a second stage for lipid accumulation under nitrogen starvation. The highest TAG yield on absorbed light was obtained at low light conditions when pre-cultivation occurred at pH 8 and lipid accumulation was induced at pH 10. However, a higher alkaline pH by itself appears not to enhance the starvation-induced increase in lipid contents, except when combined with high light and pre-cultivation occurs at those same conditions. Such strategy however also results in low biomass and TAG yields on absorbed light. Fatty acid composition analysis revealed that the relative fatty acid contents of the TAG pool are nevertheless independent from the light intensity and pH applied at either cultivation stage, suggesting a high specificity of N. oleoabundans cell machinery towards TAG production.
authors.affiliation: Wetsus - Centre of Excellence for Sustainable Water Technology, P.O. Box 1113, 8900 CC Leeuwarden, The Netherlands; Bioprocess Engineering, AlgaePARC, Wageningen University and Research Centre, P.O. Box 8129, 6700 EV Wageningen, The Netherlands. Electronic address: santos.anamarta77@gmail.com.
authors.forename: A M
authors.initials: AM
authors.lastname: Santos
authors.affiliation: Bioprocess Engineering, AlgaePARC, Wageningen University and Research Centre, P.O. Box 8129, 6700 EV Wageningen, The Netherlands.
authors.forename: R H
authors.initials: RH
authors.lastname: Wijffels
authors.affiliation: Bioprocess Engineering, AlgaePARC, Wageningen University and Research Centre, P.O. Box 8129, 6700 EV Wageningen, The Netherlands.
authors.forename: P P
authors.initials: PP
authors.lastname: Lamers
mesh_terms: D018533:Biomass; D005227:Fatty Acids / Q000378:metabolism; D006863:Hydrogen-Ion Concentration / Q000187:drug effects / Q000528:radiation effects; D008027:Light; D050356:Lipid Metabolism / Q000187:drug effects; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D009584:Nitrogen / Q000172:deficiency* / Q000494:pharmacology; D014280:Triglycerides / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D008055:Lipids; D014280:Triglycerides; D009584:Nitrogen
keywords: Light intensity; Lipids; Neochloris oleoabundans; Nitrogen starvation; pH