title: Interactions of gold-based drugs with proteins: crystal structure of the adduct formed between ribonuclease A and a cytotoxic gold(III) compound.
abstract: The reaction of Auoxo6, a dinuclear gold(III) complex, with the model protein bovine pancreatic ribonuclease is explored here by X-ray diffraction and ESI mass spectrometry. Data provide clues on the processes of adduct formation and of enzyme inhibition and, inductively, on the likely mode of action of this metallodrug.
authors.affiliation: Department of Chemistry "Ugo Schiff", Via della Lastruccia 3, 50019 Sesto Fiorentino (FI), Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Messori
authors.forename: Federica
authors.initials: F
authors.lastname: Scaletti
authors.forename: Lara
authors.initials: L
authors.lastname: Massai
authors.forename: Maria Agostina
authors.initials: MA
authors.lastname: Cinellu
authors.forename: Irene
authors.initials: I
authors.lastname: Russo Krauss
authors.forename: Giovanna
authors.initials: G
authors.lastname: di Martino
authors.forename: Alessandro
authors.initials: A
authors.lastname: Vergara
authors.forename: Luigi
authors.initials: L
authors.lastname: Paduano
authors.forename: Antonello
authors.initials: A
authors.lastname: Merlino
mesh_terms: D000818:Animals; D001310:Auranofin / Q000737:chemistry / Q000633:toxicity; D002417:Cattle; D016923:Cell Death / Q000187:drug effects; D018360:Crystallography, X-Ray; D006046:Gold / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity*; D006868:Hydrolysis / Q000187:drug effects; D008958:Models, Molecular; D012259:Ribonuclease, Pancreatic / Q000737:chemistry* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D001310:Auranofin; D006046:Gold; D012259:Ribonuclease, Pancreatic