title: Antiallergic effects of pigments isolated from green sea urchin (Strongylocentrotus droebachiensis) shells.
abstract: This study was undertaken to evaluate possible antiallergic effects of an extract of pigments from green sea urchin (Strongylocentrotus droebachiensis) shells. Effects were studied on animal models - guinea pig ileum contraction, rabbit eyes allergic conjunctivitis, and rabbit local skin irritation. The extract significantly reduced, in a dose-dependent manner, the histamine-induced contractions of the isolated guinea pig ileum with ID50 =1.2 µg/mL (in equivalents of spinochrome B), had an inhibitory effect on the model of ocular allergic inflammation surpassing the reference drug olopatadine, and did not show any irritating effect in rabbits. The extract predominantly contained polyhydroxy-1,4-naphthoquinone which would be responsible for the pharmacological activity. The active compounds of the extract were evaluated in silico with molecular docking. Molecular docking into H1R receptor structures obtained from molecular dynamic simulations showed that all spinochrome derivatives bind to the receptor active site, but spinochrome monomers fit better to it. The results of the present study suggest possibilities for the development of new agents for treating allergic diseases on the base of pigments from sea urchins shells.
authors.affiliation: Saint Petersburg Institute of Pharmacy, St. Petersburg, Russia.
authors.forename: Olga N
authors.initials: ON
authors.lastname: Pozharitskaya
authors.affiliation: Saint Petersburg Institute of Pharmacy, St. Petersburg, Russia.
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Shikov
authors.affiliation: Saint Petersburg Institute of Pharmacy, St. Petersburg, Russia.
authors.forename: Marina N
authors.initials: MN
authors.lastname: Makarova
authors.affiliation: Saint Petersburg Institute of Pharmacy, St. Petersburg, Russia.
authors.forename: Svetlana A
authors.initials: SA
authors.lastname: Ivanova
authors.affiliation: Saint Petersburg Institute of Pharmacy, St. Petersburg, Russia.
authors.forename: Vera M
authors.initials: VM
authors.lastname: Kosman
authors.affiliation: Saint Petersburg Institute of Pharmacy, St. Petersburg, Russia.
authors.forename: Valery G
authors.initials: VG
authors.lastname: Makarov
authors.affiliation: Department of Physical Chemistry, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czech Republic.
authors.forename: Václav
authors.initials: V
authors.lastname: Bazgier
authors.affiliation: Department of Physical Chemistry, Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University, Olomouc, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Berka
authors.affiliation: Department of Physical Chemistry, Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University, Olomouc, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Otyepka
authors.affiliation: Department of Medical Chemistry and Biochemistry, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic.
authors.forename: Jitka
authors.initials: J
authors.lastname: Ulrichová
mesh_terms: D060105:Animal Shells / Q000737:chemistry; D000818:Animals; D018926:Anti-Allergic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D003233:Conjunctivitis, Allergic / Q000188:drug therapy*; D003990:Dibenzoxepins / Q000494:pharmacology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D007082:Ileum / Q000187:drug effects; D008297:Male; D062105:Molecular Docking Simulation; D009285:Naphthoquinones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000069605:Olopatadine Hydrochloride; D010860:Pigments, Biological / Q000737:chemistry; D011817:Rabbits; D012867:Skin / Q000187:drug effects; D047269:Strongylocentrotus / Q000737:chemistry*
chemical_list: D018926:Anti-Allergic Agents; D003990:Dibenzoxepins; D009285:Naphthoquinones; D010860:Pigments, Biological; D000069605:Olopatadine Hydrochloride; C009304:spinochrome A; D006632:Histamine; C035342:1,4-naphthoquinone