title: Expression levels of seven candidate genes in human peripheral blood mononuclear cells and their association with preeclampsia.
abstract: OBJECTIVE
  To evaluate the peripheral blood mononuclear cell (PBMC) expression levels of hemeoxygenase 1 (HMOX-1), superoxide dismutase 1 (SOD-1), vascular endothelial growth factor A (VEGF-A), transforming growth factor beta 1 (TGF-β1), interleukin (IL)-6, IL-15 and AdipoQ genes to study their association with preeclampsia (PE).
  
  
  METHODS
  A total of 177 pregnant women were recruited: 108 cases and 69 controls. Quantification of gene expression was measured by quantitative real-time polymerase chain reaction (PCR) using TaqMan probes.
  
  
  RESULTS
  Underexpression of VEGF-A and TGF-β1 was a constant in most of the cases (80.91% and 76.36%, respectively) and their expression was associated with onset and/or severity of disease (p values < 0.05). IL-6, IL-15 and AdipoQ, showed low or no expression in PBMC samples evaluated.
  
  
  CONCLUSIONS
  PBMC underexpression of VEGF-A and TGF-β1 is a hallmark of PE in the study population.
authors.affiliation: Molecular Medicine Laboratory, Unidad Académica de Medicina Humana y Ciencias de la Salud , Zacatecas , Mexico .
authors.forename: M L
authors.initials: ML
authors.lastname: Martinez-Fierro
authors.forename: I
authors.initials: I
authors.lastname: Garza-Veloz
authors.forename: K
authors.initials: K
authors.lastname: Carrillo-Sanchez
authors.forename: V
authors.initials: V
authors.lastname: Martinez-Gaytan
authors.forename: R
authors.initials: R
authors.lastname: Cortes-Flores
authors.forename: M A
authors.initials: MA
authors.lastname: Ochoa-Torres
authors.forename: G G
authors.initials: GG
authors.lastname: Guerrero
authors.forename: I P
authors.initials: IP
authors.lastname: Rodriguez-Sanchez
authors.forename: C O
authors.initials: CO
authors.lastname: Cancela-Murrieta
authors.forename: M
authors.initials: M
authors.lastname: Zamudio-Osuna
authors.forename: J I
authors.initials: JI
authors.lastname: Badillo-Almaraz
authors.forename: C
authors.initials: C
authors.lastname: Castruita-De la Rosa
mesh_terms: D052242:Adiponectin / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D051547:Heme Oxygenase-1 / Q000378:metabolism; D006801:Humans; D019409:Interleukin-15 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D013482:Superoxide Dismutase / Q000378:metabolism; D000072105:Superoxide Dismutase-1; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D055815:Young Adult
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; D015415:Biomarkers; C569274:IL15 protein, human; C508600:IL6 protein, human; D019409:Interleukin-15; D015850:Interleukin-6; C000606290:SOD1 protein, human; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1