title: Supplementary seleno-L-methionine suppresses active cutaneous anaphylaxis reaction.
abstract: To clarify the relationship between selenium supplementation and type I allergic reaction, we investigated the effect of seleno-L-methionine (SeMet) supplementation on the active cutaneous anaphylaxis (ACA) reaction and cytokine production in splenocytes. Female BALB/c mice were sensitized by intraperitoneal injection of ovalbumin (OVA), and SeMet was administered orally for 2 weeks followed by a challenge with OVA to induce an ACA reaction. SeMet supplementation suppressed the ACA reaction in a dose-dependent manner. Plasma OVA-specific immunoglobulin E (IgE) level was strongly inhibited in SeMet-supplemented mice compared with control mice. The mRNA expression levels of the T helper 2 (Th2) cytokines interleukin (IL)-4 and IL-13 in the spleen of SeMet-supplemented mice were lower than those in control mice. The mRNA expression level of a Th1 cytokine, interferon (IFN)-γ, in the spleen of SeMet-supplemented mice was higher than that in control mice. Splenocytes restimulated with OVA in vitro from SeMet-supplemented mice produced lower amounts of IL-4 and IL-13 than those of control mice and higher amounts of IFN-γ than those from the control mice. These results suggest that oral SeMet supplementation suppresses OVA-induced ACA reaction by lowered Th2 cytokine production and augmenting Th1 cytokine production.
authors.affiliation: Department of Public Health & Preventive Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Arakawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Deguchi
authors.forename: Fumitoshi
authors.initials: F
authors.lastname: Sakazaki
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Ogino
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Okuno
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ueno
mesh_terms: D000707:Anaphylaxis / Q000188:drug therapy* / Q000378:metabolism; D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D006967:Hypersensitivity / Q000188:drug therapy* / Q000378:metabolism; D007073:Immunoglobulin E / Q000097:blood; D008099:Liver / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism; D012645:Selenomethionine / Q000097:blood / Q000493:pharmacokinetics / Q000627:therapeutic use*; D012882:Skin Tests; D013154:Spleen / Q000166:cytology / Q000378:metabolism
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D007073:Immunoglobulin E; D010047:Ovalbumin; D012645:Selenomethionine