title: A new ternary copper(II) complex derived from 2-(2'-pyridyl)benzimidazole and glycylglycine: synthesis, characterization, DNA binding and cleavage, antioxidation and HSA interaction.
abstract: A new ternary copper(II)-dipeptide complex [Cu(glygly)(HPB)(Cl)]⋅2H2O (glygly=glycylglycine anion, HPB=2-(2'-pyridyl)benzimidazole) has been synthesized and characterized. The DNA interaction of the complex was studied by spectroscopic methods, viscosity, and electrophoresis measurements. The antioxidant activity was also investigated using the pyrogallol autoxidation assay. Besides, the interaction of the complex with human serum albumin (HSA) in vitro was examined by multispectroscopic techniques. The complex partially intercalated to CT-DNA with a high binding constant (Kb=7.28×10(5) M(-1)), and cleaved pBR322 DNA efficiently via an oxidative mechanism in the presence of Vc, with the HO· and O2(-) as the active species, and the SOD as a promoter. Furthermore, the complex shows a considerable SOD-like activity with the IC50 value of 3.8386 μM. The complex exhibits desired binding affinity to HSA, in which hydrogen bond or vander Waals force played a major role. The alterations of HSA secondary structure induced by the complex were confirmed by UV-visible, CD, synchronous fluorescence and 3D fluorescence spectroscopy.
authors.affiliation: Department of Applied Chemistry, South China Agricultural University, Guangzhou 510642, PR China.
authors.forename: Xia-Bing
authors.initials: XB
authors.lastname: Fu
authors.affiliation: Department of Applied Chemistry, South China Agricultural University, Guangzhou 510642, PR China.
authors.forename: Zi-Hua
authors.initials: ZH
authors.lastname: Lin
authors.affiliation: Department of Applied Chemistry, South China Agricultural University, Guangzhou 510642, PR China; Institute of Biomaterial, South China Agricultural University, Guangzhou 510642, PR China.
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Liu
authors.affiliation: Department of Applied Chemistry, South China Agricultural University, Guangzhou 510642, PR China; Institute of Biomaterial, South China Agricultural University, Guangzhou 510642, PR China. Electronic address: lexyfu@163.com.
authors.forename: Xue-Yi
authors.initials: XY
authors.lastname: Le
mesh_terms: D000818:Animals; D001562:Benzimidazoles / Q000138:chemical synthesis* / Q000737:chemistry; D001667:Binding, Competitive; D002417:Cattle; D002942:Circular Dichroism; D003300:Copper / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D053837:DNA Cleavage; D004587:Electrophoresis, Agar Gel; D004735:Energy Transfer; D004996:Ethidium / Q000378:metabolism; D006033:Glycylglycine / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D020128:Inhibitory Concentration 50; D007700:Kinetics; D008024:Ligands; D010084:Oxidation-Reduction; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics; D014783:Viscosity
chemical_list: C479869:2-(2'-pyridyl)benzimidazole; D001562:Benzimidazoles; D008024:Ligands; D012709:Serum Albumin; D006033:Glycylglycine; D003300:Copper; D004247:DNA; C427203:calf thymus DNA; D004996:Ethidium
keywords: 2-(2′-pyridyl)benzimidazole; Antioxidant activity; Copper(II)–dipeptide complex; DNA binding; HSA interaction; Oxidative cleavage