title: Quantitative laser speckle flowmetry of the in vivo microcirculation using sidestream dark field microscopy.
abstract: We present integrated Laser Speckle Contrast Imaging (LSCI) and Sidestream Dark Field (SDF) flowmetry to provide real-time, non-invasive and quantitative measurements of speckle decorrelation times related to microcirculatory flow. Using a multi exposure acquisition scheme, precise speckle decorrelation times were obtained. Applying SDF-LSCI in vitro and in vivo allows direct comparison between speckle contrast decorrelation and flow velocities, while imaging the phantom and microcirculation architecture. This resulted in a novel analysis approach that distinguishes decorrelation due to flow from other additive decorrelation sources.
authors.affiliation: Department of Biomedical Engineering and Physics, Academic Medical Center, University of Amsterdam, P.O. Box 22700, 1100 DE Amsterdam, The Netherlands ; MQ Biofocus Research Centre, Macquarie University, NSW, Sydney 2109, Australia.
authors.forename: Annemarie
authors.initials: A
authors.lastname: Nadort
authors.forename: Rutger G
authors.initials: RG
authors.lastname: Woolthuis
authors.forename: Ton G
authors.initials: TG
authors.lastname: van Leeuwen
authors.forename: Dirk J
authors.initials: DJ
authors.lastname: Faber
keywords: (170.0180) Microscopy; (170.2655) Functional monitoring and imaging; (170.3880) Medical and biological imaging; (170.6480) Spectroscopy, speckle