title: Exogenous bFGF or TGFβ1 accelerates healing of reconstructed dura by CO2 laser soldering in minipigs.
abstract: This study aims to explore the probable mechanism of better result of dural reconstruction by CO2 laser soldering and the effect of exogenous basic fibroblast growth factor (bFGF) or transforming growth factor-beta1(TGFβ1) on wound healing. In part I of the study, ten minipigs were randomized into two equal groups, and the dural defects were reconstructed by conventional fibrin glue (FG) bonding (group I a) or by CO2 laser soldering (group Ib). In part II, 36 minipigs were randomized into three equal groups, and the dural defect was reconstructed by CO2 laser soldering; then exogenous bFGF or TGFβ1 was administered in group IIb and group IIc, respectively, while group IIa served as control group. The dural specimens were harvested at 1st week postoperatively in part I; and at 1st, 2nd, 3rd, and 4th week postoperatively in part II, they were examined for healing condition and subjected to hematoxylin-eosin (HE) staining and immunohistochemical (IHC) staining with antibodies against bFGF and TGFβ1. In part I, group Ib showed higher fibroblast cell density than group Ia (P < 0.05). The optical density (OD) for IHC staining with antibodies against bFGF of group Ib was significantly higher than that of group Ia (P < 0.05), and for IHC staining with antibodies against TGFβ1, group Ib showed positive staining while group Ia was negative. In part II, administering exogenous bFGF or TGFβ1 made a left shift of fibroblast cell number-time curve compared with control group. For specimens' IHC staining with antibodies against bFGF, the OD of group IIb was higher than that of group IIa in the corresponding time. For specimens' IHC staining with antibodies against TGFβ1, the OD of groups IIb and IIc was both higher than that of group IIa (P < 0.05 and P < 0.01, respectively). In conclusion, CO2 laser may trigger fibroblast proliferation through stimulating the secretion of bFGF and TGFβ1. Topically administering exogenous bFGF or TGFβ1 could accelerate the healing of the reconstructed dura by enhancing secretion of bFGF and/or TGFβ1 and promoting the process of fibroblast gathering-degrading.
authors.affiliation: Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, 6 Xili, Tiantan, Dongcheng District, Beijing, 100050, People's Republic of China.
authors.forename: Zhenmin
authors.initials: Z
authors.lastname: Wang
authors.forename: Hongliang
authors.initials: H
authors.lastname: Zhong
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Yang
authors.forename: Fu
authors.initials: F
authors.lastname: Zhao
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Peiran
authors.initials: P
authors.lastname: Qu
authors.forename: Pinan
authors.initials: P
authors.lastname: Liu
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D007150:Immunohistochemistry; D054020:Lasers, Gas; D008297:Male; D019651:Plastic Surgery Procedures; D012886:Skull / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D013552:Swine; D013556:Swine, Miniature; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects* / Q000528:radiation effects*
chemical_list: D000906:Antibodies; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2