title: Transcription through enhancers suppresses their activity in Drosophila.
abstract: BACKGROUND
  Enhancer elements determine the level of target gene transcription in a tissue-specific manner, providing for individual patterns of gene expression in different cells. Knowledge of the mechanisms controlling enhancer action is crucial for understanding global regulation of transcription. In particular, enhancers are often localized within transcribed regions of the genome. A number of experiments suggest that transcription can have both positive and negative effects on regulatory elements. In this study, we performed direct tests for the effect of transcription on enhancer activity.
  
  
  RESULTS
  Using a transgenic reporter system, we investigated the relationship between the presence of pass-through transcription and the activity of Drosophila enhancers controlling the expression of the white and yellow genes. The results show that transcription from different promoters affects the activity of enhancers, counteracting their ability to activate the target genes. As expected, the presence of a transcriptional terminator between the inhibiting promoter and the affected enhancer strongly reduces the suppression. Moreover, transcription leads to dislodging of the Zeste protein that is responsible for the enhancer-dependent regulation of the white gene, suggesting a 'transcription interference' mechanism for this regulation.
  
  
  CONCLUSIONS
  Our findings suggest a role for pass-through transcription in negative regulation of enhancer activity.
authors.forename: Maksim
authors.initials: M
authors.lastname: Erokhin
authors.forename: Anna
authors.initials: A
authors.lastname: Davydova
authors.forename: Alexander
authors.initials: A
authors.lastname: Parshikov
authors.forename: Vasily M
authors.initials: VM
authors.lastname: Studitsky
authors.forename: Pavel
authors.initials: P
authors.lastname: Georgiev
authors.forename: Darya
authors.initials: D
authors.lastname: Chetverina