title: ANXA2 regulates the behavior of SGC-7901 cells.
abstract: ANXA2, a member of the annexin family, is overexpressed and plays important roles in tumor development. However, the significance of ANXA2 expression in gastric carcinoma has not been clarified.To elucidate its roles in growth of gastric cancer, ANXA2 expression in SGC-7901 cells was inhibited with a designated siRNA, then cell proliferation, cell cycling, apoptosis and motility were determined by MTT assay, flow cytometry, Hoechst 33342 staining and wound healing assay, respectively. To further assess the behavior of ANXA2 deleted SGC- 7901 cells, changes of microstructures were observed under fluorescence microscopy, laser scanning confocal microscopy and electron microscopy. We found that inhibition of ANXA2 expression caused cell proliferation to decrease significantly with G1 arrest, motility to be reduced with changes in pseudopodia/filopodia structure and F-actin and β-tubulin expression, and apoptosis to be enhanced albeit without significance. At the same time, ANXA2 deletion resulted in fewer pseudopodia/filopodia, non-stained areas were increased, contact inhibition among cells reappeared, and expression of F-actin and β-tubulin was decreased, with induction of polymerized disassembled forms. Taken together, these data suggest that ANXA2 overexpression is important to maintain the malignancy of cancer cells, and this member of the annexin family has potential to be considered as a target for the gene therapy of gastric carcinoma.
authors.affiliation: The Lab of Tumor Molecular Cellular Biology, Shaanxi Normal University, Xi'an, Shaanxi,China E-mail : ychhou@snnu.edu.cn.
authors.forename: Meng-Yao
authors.initials: MY
authors.lastname: Sun
authors.forename: Rui-Huan
authors.initials: RH
authors.lastname: Xing
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Gao
authors.forename: Xiang
authors.initials: X
authors.lastname: Yu
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: He
authors.forename: Ning
authors.initials: N
authors.lastname: Gao
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Shi
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Hu
authors.forename: Qi-Xuan
authors.initials: QX
authors.lastname: Wang
authors.forename: Jin-Hui
authors.initials: JH
authors.lastname: Xu
authors.forename: Ying-Chun
authors.initials: YC
authors.lastname: Hou
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D017306:Annexin A2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis; D015153:Blotting, Western; D002453:Cell Cycle; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D014404:Tubulin / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014945:Wound Healing
chemical_list: C472822:ANXA2 protein, human; D000199:Actins; D017306:Annexin A2; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014404:Tubulin