title: Surface display of human growth hormone on Bacillus subtilis spores for oral administration.
abstract: Human growth hormone (hGH) is the major and important hormone component of human being. At present, hGH for clinical uses is mostly produced in Escherichia coli, which requires costly denaturation and refolding to recover functionality. To obtain long-term bioactive hormone, we used hGH as a foreign gene and constructed a recombinant plasmid pJS700-hGH which carries a recombinant gene cotC-hgh with an enterokinase site under the control of cotC promoter. Plasmid pJS700-hGH was transformed into Bacillus subtilis by double crossover and an amylase-inactivated mutant was produced. After spore formation, Western blot and fluorescence immunoassay were used to monitor hGH surface expression on spores. Oral administration to silkworm with spores displaying hGH further showed that the recombinant spores may have potential ability to be digested and absorbed into the silkworm's hemolymph due to both the resistant characters of spores and the addition of enterokinase site.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu, People's Republic of China, c.q.lian@163.com.
authors.forename: Chaoqun
authors.initials: C
authors.lastname: Lian
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Fan
authors.initials: F
authors.lastname: Feng
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Qi
authors.initials: Q
authors.lastname: Tang
authors.forename: Qin
authors.initials: Q
authors.lastname: Yao
authors.forename: Keping
authors.initials: K
authors.lastname: Chen
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001412:Bacillus subtilis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012831:Bombyx; D061505:Cell Surface Display Techniques; D003001:Cloning, Molecular; D004765:Enteropeptidase / Q000235:genetics; D019382:Human Growth Hormone / Q000008:administration & dosage* / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000096:biosynthesis / Q000235:genetics; D013170:Spores / Q000737:chemistry / Q000378:metabolism; D014162:Transfection
chemical_list: D011993:Recombinant Fusion Proteins; D019382:Human Growth Hormone; D004765:Enteropeptidase