title: Analysis of the multicopper oxidase gene regulatory network of Aeromonas hydrophila.
abstract: Multicopper oxidase (MCO) is an enzyme which involves in reducing the oxygen in a four electron reduction to water with concomitant one electron oxidation of reducing the substrate. We have generated the 3-D structure of MCO by homology modeling and validated on the basis of free energy while 90.4 % amino acid residues present in allowed regions of Ramachandran plot. The screening of potential hazardous aromatic compounds for MCO was performed using molecular docking. We obtained Sulfonaphthal, Thymolphthalein, Bromocresol green and Phloretin derivatives of phenol and aromatic hydrocarbon were efficient substrates for MCO. The phylogeny of MCO reveals that other bacteria restrain the homologous gene of MCO may play an important role in biodegradation of aromatic compounds. We have demonstrated the gene regulatory network of MCO with other cellular proteins which play a key role in gene regulation. These findings provide a new insight for oxidization of phenolic and aromatic compounds using biodegradation process for controlling environmental pollution.
authors.affiliation: Institute of Systems and Synthetic Biology, Universite d'Evry Val d'Essonne-Genopole-CNRS UPS3201, Batiment Geneavenir 6, Genopole Campus 1, 5 rue Henri Desbruères, 91030 Evry Cedex, France ; National Bureau of Fish Genetic Resources, Canal Ring Road, PO-Dilkusha, Lucknow, 226002 India.
authors.forename: Vijai
authors.initials: V
authors.lastname: Singh
authors.forename: Indra
authors.initials: I
authors.lastname: Mani
authors.forename: Dharmendra Kumar
authors.initials: DK
authors.lastname: Chaudhary
keywords: Biodegradation; Docking; Gene regulatory network; Homology modeling; Multicopper oxidase