title: Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens.
abstract: High frequency transformation of Arabidopsis thaliana leaf explants has been obtained using a disarmed Ti plasmid containing the coding region of a neomycin phosphotransferase gene (NPT II) as a selectable marker. The rate of transformation ranged from 55 to 63 percent when acetosyringone (AS), a natural wound response molecule, was added to an Agrobacterium tumefaciens culture prior to incubation with leaf segments. Without acetosyringone, the transformation rate was approximately 2 to 3 percent. Calli resistant to G418 were regenerated into mature flowering plants in the presence of 10 μg/ml G418. Southern analysis and neomycin phosphotransferase assays confirmed the insertion and expression of the NPT II gene in regenerated Arabidopsis plants.
authors.affiliation: Molecular Biology Department, Zoecon Research Institute, Sandoz Crop Protection Corporation, 975 California Avenue, 94304, Palo Alto, CA, USA.
authors.forename: S N
authors.initials: SN
authors.lastname: Sheikholeslam
authors.forename: D P
authors.initials: DP
authors.lastname: Weeks