title: IGZO thin film transistor biosensors functionalized with ZnO nanorods and antibodies.
abstract: We demonstrate a biosensor structure consisting of an IGZO (Indium-Gallium-Zinc-Oxide) TFT (thin film transistor) and an extended sensing pad. The TFT acts as the sensing and readout device, while the sensing pad ensures the isolation of biological solution from the transistor channel layer, and meanwhile increases the sensing area. The biosensor is functionalized by first applying ZnO nanorods to increase the surface area for attracting electrical charges of EGFR (epidermal growth factor receptor) antibodies. The device is able to selectively detect 36.2 fM of EGFR in the total protein solution of 0.1 ng/ml extracted from squamous cell carcinoma (SCC). Furthermore, the conjugation duration of the functionalized device with EGFR can be limited to 3 min, implying that the biosensor has the advantage for real-time detection.
authors.affiliation: Graduate Institute of Photonics and Optoelectronics, National Taiwan University, 1, Roosevelt Road, Section 4, Taipei 106, Taiwan.
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Shen
authors.affiliation: Graduate Institute of Photonics and Optoelectronics, National Taiwan University, 1, Roosevelt Road, Section 4, Taipei 106, Taiwan.
authors.forename: Chun-Hsu
authors.initials: CH
authors.lastname: Yang
authors.affiliation: Graduate Institute of Photonics and Optoelectronics, National Taiwan University, 1, Roosevelt Road, Section 4, Taipei 106, Taiwan.
authors.forename: Shu-Wen
authors.initials: SW
authors.lastname: Chen
authors.affiliation: Graduate Institute of Photonics and Optoelectronics, National Taiwan University, 1, Roosevelt Road, Section 4, Taipei 106, Taiwan.
authors.forename: Shou-Hao
authors.initials: SH
authors.lastname: Wu
authors.affiliation: Department of Otolaryngology, National Taiwan University Hospital and College of Medicine, National Taiwan University, No. 1, Jen-Ai Road, Section 1, Taipei 100, Taiwan; Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, No. 1, Jen-Ai Road, Section 1, Taipei 100, Taiwan. Electronic address: tlyang@ntu.edu.tw.
authors.forename: Tsung-Lin
authors.initials: TL
authors.lastname: Yang
authors.affiliation: Graduate Institute of Photonics and Optoelectronics, National Taiwan University, 1, Roosevelt Road, Section 4, Taipei 106, Taiwan; Department of Electrical Engineering, National Taiwan University, 1, Roosevelt Road, Section 4, Taipei 106, Taiwan. Electronic address: jjhuang@cc.ee.ntu.edu.tw.
authors.forename: Jian-Jang
authors.initials: JJ
authors.lastname: Huang
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D002460:Cell Line; D045744:Cell Line, Tumor; D004867:Equipment Design; D005708:Gallium / Q000737:chemistry; D006801:Humans; D007204:Indium / Q000737:chemistry; D057230:Limit of Detection; D043942:Nanotubes / Q000737:chemistry*; D014173:Transistors, Electronic; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D007204:Indium; C038863:gallium oxide; C047711:indium oxide; D005708:Gallium; D015034:Zinc Oxide
keywords: Biosensors; Nanorods; Thin film transistors