title: Compositional differences in commercially available prothrombin complex concentrates.
abstract: Prothrombin complex concentrates (PCCs) are used in the management of bleeding complications with conventional oral anticoagulant drugs. Since the activation of these complexes results in the generation of factor Xa and IIa, these agents may potentially neutralize the newly developed Xa and thrombin inhibitors. Although the potency of these agents is defined in units that represent the level of factor IX (FIX), other factors including factor II, VII, and X are also present. Protein C, protein S, and protein Z are also present. The purpose of this study is to compare the compositional differences in the currently available PCCs along with the older agents. Measurement of compositional parameters including protein and FIX, mass spectrometric analysis of the native and activated PCCs, Western blotting studies on the native and activated PCCs for the activation products including thrombin, and their effect on blood and plasma coagulation parameters were carried out.
authors.affiliation: 1Department of Pathology, Loyola University Medical Center, Maywood, IL, USA.
authors.forename: Nasir
authors.initials: N
authors.lastname: Sadeghi
authors.forename: Daniel
authors.initials: D
authors.lastname: Kahn
authors.forename: Daneyal
authors.initials: D
authors.lastname: Sayed
authors.forename: Debra
authors.initials: D
authors.lastname: Hoppenstadt
authors.forename: Walter
authors.initials: W
authors.lastname: Jeske
authors.forename: Job
authors.initials: J
authors.lastname: Harenberg
authors.forename: Phillip
authors.initials: P
authors.lastname: Dechristopher
authors.forename: Jawed
authors.initials: J
authors.lastname: Fareed
mesh_terms: D001779:Blood Coagulation Factors / Q000737:chemistry*; D001780:Blood Coagulation Tests; D005164:Factor IX / Q000378:metabolism; D006493:Heparin / Q000737:chemistry; D006801:Humans; D015151:Immunoblotting; D013058:Mass Spectrometry / Q000379:methods; D013917:Thrombin / Q000378:metabolism; D013925:Thromboplastin / Q000032:analysis
chemical_list: D001779:Blood Coagulation Factors; C025667:prothrombin complex concentrates; D005164:Factor IX; D006493:Heparin; D013925:Thromboplastin; D013917:Thrombin
keywords: Innovin; prothrombin complex concentrates; recombiplastin 2G; tissue factors; vitamin K antagonist