title: Expression of herpes simplex virus 1 microRNAs in cell culture models of quiescent and latent infection.
abstract: To facilitate studies of herpes simplex virus 1 latency, cell culture models of quiescent or latent infection have been developed. Using deep sequencing, we analyzed the expression of viral microRNAs (miRNAs) in two models employing human fibroblasts and one using rat neurons. In all cases, the expression patterns differed from that in productively infected cells, with the rat neuron pattern most closely resembling that found in latently infected human or mouse ganglia in vivo.
authors.affiliation: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Igor
authors.initials: I
authors.lastname: Jurak
authors.forename: Michael
authors.initials: M
authors.lastname: Hackenberg
authors.forename: Ju Youn
authors.initials: JY
authors.lastname: Kim
authors.forename: Jean M
authors.initials: JM
authors.lastname: Pesola
authors.forename: Roger D
authors.initials: RD
authors.lastname: Everett
authors.forename: Chris M
authors.initials: CM
authors.lastname: Preston
authors.forename: Angus C
authors.initials: AC
authors.lastname: Wilson
authors.forename: Donald M
authors.initials: DM
authors.lastname: Coen
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D005347:Fibroblasts / Q000378:metabolism; D006561:Herpes Simplex / Q000235:genetics / Q000378:metabolism*; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D051381:Rats; D017735:Virus Latency / Q000235:genetics*
chemical_list: D035683:MicroRNAs