title: Surface functionalized mesoporous silica nanoparticles with natural proteins for reduced immunotoxicity.
abstract: Mesoporous silica nanoparticles (MSNs) present themselves as one of the most promising nano-carriers for drug delivery. To reduce their immunotoxicities, in this study, natural proteins of gelatin (Gel), bovine serum albumin (BSA), and lysozyme (Lys) were employed as end-caps of MSNs by using succinic anhydride as an intermediate linker, thus leading to fabrication of MSNs/protein nanocomposites, respectively. Furthermore, combined techniques of SEM, TEM, FTIR, and zeta potential instruments were utilized to monitor the construction processes of MSNs/protein nanocomposites, respectively. Finally, the immunotoxicities of those nanocomposites to macrophage cells (RAW264.7 cells) were investigated in detail, i.e., cell morphology, cell viability, nitric oxide (NO) production, reactive oxygen species (ROS), and acid phosphatase activity (ACP) as well as inflammation cytokine expressions (tumor necrosis factor-α and interleukin-1β). All results suggest that macrophages were activated after uptaking nanoparticles of SiO2 and MSNs, which subsequently induced severe inflammation responses in vitro. In contrast, the inflammation responses of MSNs nanocomposites were reduced dramatically after end-capping with those natural proteins. Overall, this study accumulates knowledge for the development of MSNs-based drug delivery systems with reduced immunotoxicity.
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, People's Republic of China; College of Material Science and Engineering, Chongqing University, Chongqing, 400044, People's Republic of China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Luo
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Renlong
authors.initials: R
authors.lastname: Xin
authors.forename: Beilu
authors.initials: B
authors.lastname: Zhang
authors.forename: Jinghua
authors.initials: J
authors.lastname: Li
authors.forename: Xingwei
authors.initials: X
authors.lastname: Ding
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Hou
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Kaiyong
authors.initials: K
authors.lastname: Cai
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D004337:Drug Carriers / Q000737:chemistry / Q000494:pharmacology; D005780:Gelatin / Q000737:chemistry*; D008264:Macrophages / Q000378:metabolism; D008422:Materials Testing; D051379:Mice; D009113:Muramidase / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D016062:Porosity; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D005780:Gelatin; D009113:Muramidase
keywords: immunotoxicity; inflammation responses; macrophages; mesoporous silica nanoparticles; natural proteins