title: Binding of colchicine to a soluble fraction of carrot cells grown in suspension culture.
abstract: The binding of [(3)H]colchicine to soluble component prepared from carrot (Daucus carota L.) cells in suspension culture was assayed by the diethylaminoethyl(DEAE)-cellulose powder method. The binding activity was very labile and the time course of the binding indicated that the colchicine-bound complex was also unstable. The reaction was enhanced by vinblastine but lumicolchicine had no effect. The optimum temperature for the reaction was 30° C, and the colchicine binding constant was calculated to be 3.5·10(4) l mol(-1) at 30° C.
authors.affiliation: Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Sugitani, 930-01, Toyama, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Okamura