title: A coumarin derivative as a fluorogenic glycoproteomic probe for biological imaging.
abstract: Fluorescence imaging in living cells is typically carried out using a functionalized fluorescent dye. But it often causes strong background noise under many conditions where washing is not applicable. Here, we report on a coumarin based fluorogenic probe, which can be used as a bioorthogonal-labeling tool for glycoproteins. The results indicated that the probe was able to image glycoproteins in living cells and it may also be suitable for intracellular imaging.
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, P. R. China. xz-zhang@whu.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Rong
authors.forename: Li-Han
authors.initials: LH
authors.lastname: Liu
authors.forename: Si
authors.initials: S
authors.lastname: Chen
authors.forename: Han
authors.initials: H
authors.lastname: Cheng
authors.forename: Chang-Sheng
authors.initials: CS
authors.lastname: Chen
authors.forename: Ze-Yong
authors.initials: ZY
authors.lastname: Li
authors.forename: Si-Yong
authors.initials: SY
authors.lastname: Qin
authors.forename: Xian-Zheng
authors.initials: XZ
authors.lastname: Zhang
mesh_terms: D003374:Coumarins / Q000737:chemistry*; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008954:Models, Biological; D057054:Molecular Imaging / Q000295:instrumentation*
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; D006023:Glycoproteins; C030123:coumarin