title: Do GSTT1 and GSTM1 polymorphisms influence intoxication events in individuals occupationally exposed to pesticides?
abstract: This study evaluated the variability of GSTM1 and GSTT1 polymorphisms in individuals occupationally exposed to pesticides in ten Goias municipalities that present intense agricultural activity. We evaluated blood samples of 235 individuals, which 120 were rural workers occupationally exposed to pesticides and 115 formed the control group, analyzing GST polymorphisms by quantitative polymerase chain reaction (qPCR).The exposed group consisted of 111 men and nine women only getting an average of 39 ± 9 years. These workers were from ten rural municipalities situated at Goias state. It was found that 18 % of the exposed individuals had the GSTT1 null genotype and 49 % had the GSTM1 null genotype, and 10 % had both null genotypes. Data as intoxication (42 %), use of Personal Protection Equipment (PPE; 52 %) and if the worker prepared the pesticide (7 %), or if just applied the pesticide (22 %) or if the worker prepared and applied (71 %) have all been correlated with genetic polymorphisms. There were no statistically significant differences between the GSTM1 and GSTT1 polymorphisms between control and exposed groups. Finally, we could not associate a null GSTT1 or null GSTM1 polymorphisms or both to intoxication events caused by pesticides, but instead we presented the importance to use PPE to prevent such harm, once we found a statistically significant association between the use of PPE and events of intoxication (p ≤ 0.001).
authors.affiliation: Departamento de Biologia, Núcleo de Pesquisas Replicon, Rua 235, n. 40, Goiânia, Brazil.
authors.forename: Fernanda Ribeiro
authors.initials: FR
authors.lastname: Godoy
authors.forename: Emília Oliveira Alves
authors.initials: EO
authors.lastname: Costa
authors.forename: Angela Adamski
authors.initials: AA
authors.lastname: da Silva Reis
authors.forename: Mariana Pedrosa
authors.initials: MP
authors.lastname: Batista
authors.forename: Aldaires Vieira
authors.initials: AV
authors.lastname: de Melo
authors.forename: Macks Wendhel
authors.initials: MW
authors.lastname: Gonçalves
authors.forename: Alex Silva
authors.initials: AS
authors.lastname: Cruz
authors.forename: Caroline Oliveira
authors.initials: CO
authors.lastname: de Araújo Melo
authors.forename: Lysa Bernardes
authors.initials: LB
authors.lastname: Minasi
authors.forename: Cristiano Luiz
authors.initials: CL
authors.lastname: Ribeiro
authors.forename: Aparecido Divino
authors.initials: AD
authors.lastname: da Cruz
authors.forename: Daniela
authors.initials: D
authors.lastname: de Melo E Silva
mesh_terms: D000328:Adult; D000383:Agriculture; D000395:Air Pollutants, Occupational / Q000633:toxicity*; D000428:Alcohol Drinking / Q000235:genetics; D001938:Brazil; D005260:Female; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects*; D010575:Pesticides / Q000633:toxicity*; D011110:Polymorphism, Genetic; D012907:Smoking / Q000235:genetics
chemical_list: D000395:Air Pollutants, Occupational; D005982:Glutathione Transferase; D010575:Pesticides; C413545:glutathione S-transferase T1