title: Pharmacokinetic study of cinnamaldehyde in rats by GC-MS after oral and intravenous administration.
abstract: A selective and sensitive method utilizing gas chromatography-mass spectrometry was developed for simultaneous determination of cinnamaldehyde, cinnamyl alcohol, and methyl cinnamate in rat plasma. Cinnamaldehyde and cinnamyl alcohol can inter-convert to one another in rats, thus simultaneous quantifying both analytes provided a reliable and accurate method of assessment. Three qualifying ions (131 m/z, 105 m/z and 92 m/z) were chosen for simultaneous quantification of cinnamaldehyde and its metabolites. In this study, the calibration curves demonstrated a good linearity and reproducibility over the range of 20-2000ng/ml (r(2)≥0.999) for all analytes. Furthermore, the sensitivity of gas chromatography-mass spectrometry revealed sufficient lower limit of quantitation and detection of 20ng/ml and 5ng/ml, respectively, in the pharmacokinetic analysis. The intra- and inter-day precision variations were less than 10.4% and 12.2%, respectively, whilst accuracy values ranged from -8.6% to 14.8%. All analytes were stable in plasma and in processed samples at room temperature for 24h with no significant degradation after three freeze/thaw cycles. A small amount of the administered cinnamaldehyde had long half-life of 6.7±1.5h. In this study, gas chromatography-mass spectrometry was demonstrated to be a powerful tool for the pharmacokinetic studies of rats after intravenous and oral administration of cinnamaldehyde.
authors.affiliation: Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China; The Cultivation Project of Collaborative Innovation Center for Chinese Medicine in QinBa Mountains, Xi'an 710032, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China; The Cultivation Project of Collaborative Innovation Center for Chinese Medicine in QinBa Mountains, Xi'an 710032, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China; The Cultivation Project of Collaborative Innovation Center for Chinese Medicine in QinBa Mountains, Xi'an 710032, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Team NO. 1, Cadet Brigade, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China; The Cultivation Project of Collaborative Innovation Center for Chinese Medicine in QinBa Mountains, Xi'an 710032, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Institute for Drug and Instrument Control of Xinjiang Military Region, Urumqi 830063, China.
authors.forename: Honghai
authors.initials: H
authors.lastname: Tu
authors.affiliation: Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China; The Cultivation Project of Collaborative Innovation Center for Chinese Medicine in QinBa Mountains, Xi'an 710032, China. Electronic address: caowei@fmmu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.affiliation: Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China; The Cultivation Project of Collaborative Innovation Center for Chinese Medicine in QinBa Mountains, Xi'an 710032, China. Electronic address: wangsiw@fmmu.edu.cn.
authors.forename: Siwang
authors.initials: S
authors.lastname: Wang
mesh_terms: D000171:Acrolein / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics; D061605:Administration, Intravenous; D000284:Administration, Oral; D000818:Animals; D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006207:Half-Life; D008297:Male; D020005:Propanols / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
chemical_list: D020005:Propanols; D000171:Acrolein; C012843:cinnamaldehyde; C020722:cinnamyl alcohol
keywords: Bioavailability; Cinnamaldehyde; GC–MS; Methyl cinnamate; Pharmacokinetic