title: Molecular evaluation of proliferative-phase endometrium may provide insight about the underlying causes of infertility in women with endometriosis.
abstract: OBJECTIVE
  To determine if endometrial gene expression is different in women with endometriosis-related infertility and fertile women.
  
  
  METHODS
  Prospective study of mid-follicular phase endometrium in 47 subjects in two phases: microarray study of 10 infertile women with endometriosis and five fertile controls, and a quantitative real-time PCR (qRT-PCR) study of 27 infertile women with endometriosis and 15 fertile controls. Gene expression was determined by DNA microarray, and qRT-PCR used for 12 "promising" genes based on the microarray analysis.
  
  
  RESULTS
  Compared to fertile controls, women with stage I-II endometriosis had 23, and women with stage III-IV had 35 genes that were significantly up- or down-regulated by microarray. However, using qRT-PCR, only chemokine ligand (CXCL) 13 was significantly down-regulated and somatostatin was significantly up-regulated with early endometriosis, and only CXCL 14 was significantly down-regulated with advanced endometriosis compared to fertile controls.
  
  
  CONCLUSIONS
  Our findings indicate that the pattern of gene expression in proliferative-phase endometrium is different when comparing tissue from patients with endometriosis versus fertile controls. Recognition of these endometrial alterations could be helpful to diagnose and stage endometriosis, and may provide insight to explain why conception rates are low in women with endometriosis.
authors.affiliation: Department of Obstetrics and Gynecology, Carolinas Medical Center, 1025 Morehead Medical Dr., Suite 500, Charlotte, NC, 28204, USA, bhurst@carolinas.org.
authors.forename: Bradley S
authors.initials: BS
authors.lastname: Hurst
authors.forename: Kathleen E
authors.initials: KE
authors.lastname: Shimp
authors.forename: Mollie
authors.initials: M
authors.lastname: Elliot
authors.forename: Paul B
authors.initials: PB
authors.lastname: Marshburn
authors.forename: Judy
authors.initials: J
authors.lastname: Parsons
authors.forename: Zahra
authors.initials: Z
authors.lastname: Bahrani-Mostafavi
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D054382:Chemokine CXCL13 / Q000235:genetics / Q000378:metabolism*; D004715:Endometriosis / Q000150:complications / Q000235:genetics*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D005498:Follicular Phase / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007247:Infertility, Female / Q000209:etiology / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D054382:Chemokine CXCL13