title: Intranasal immunization of mice with inactivated virus and mast cell activator C48/80 elicits protective immunity against influenza H1 but not H5.
abstract: Vaccination represents the most economic and effective strategy of preventing influenza pandemics. We previously demonstrated that intranasal immunization of mice with recombinant hemagglutinin and the mast cell activator C48/80 elicited protective immunity against challenge with the 2009 pandemic H1N1 influenza in mice, demonstrating that the novel C48/80 mucosal adjuvant was safe and effective. The present study demonstrated that intranasal immunization with inactivated H1N1 virus and C48/80 elicited protective immunity against lethal challenge with homologous virus, however, when the immunogen was replaced with inactivated H5N1 virus protection was lost. These observations suggested that the adjuvant effects conferred by C48/80 were virus subtype specific and that its use as a broad-spectrum adjuvant for use in immunizations against all influenza viruses needs to be further analyzed.
authors.affiliation: Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center, Peking Union Medical Collage (PUMC); Key Laboratory of Human Disease Comparative Medicine, Ministry of Health; Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine , Beijing, 100021 , China.
authors.forename: Lili
authors.initials: L
authors.lastname: Xu
authors.forename: Linlin
authors.initials: L
authors.lastname: Bao
authors.forename: Fengdi
authors.initials: F
authors.lastname: Li
authors.forename: Qi
authors.initials: Q
authors.lastname: Lv
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Deng
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Yao
authors.forename: Pin
authors.initials: P
authors.lastname: Yu
authors.forename: Chuan
authors.initials: C
authors.lastname: Qin
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000281:Administration, Intranasal; D000818:Animals; D005260:Female; D006801:Humans; D007114:Immunization; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines; D007251:Influenza, Human / Q000276:immunology / Q000517:prevention & control*; D008407:Mast Cells / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control*; D011108:Polymers / Q000008:administration & dosage*; D015164:Vaccines, Inactivated
chemical_list: D000276:Adjuvants, Immunologic; D007252:Influenza Vaccines; D011108:Polymers; D015164:Vaccines, Inactivated