title: Synthetic prion Peptide 106-126 resulted in an increase matrix metalloproteinases and inflammatory cytokines from rat astrocytes and microglial cells.
abstract: It has been shown that the accumulation of prion in the cytoplasm can result in neurodegenerative disorders. Synthetic prion peptide 106-126 (PrP) is a glycoprotein that is expressed predominantly by neurons and other cells, including glial cells. Prion-induced chronic neurodegeneration has a substantial inflammatory component, and an increase in the levels of matrix metalloproteinases (MMPs) may play an important role in neurodegenerative development and progression. However, the expression of MMPs in PrP induced rat astrocytes and microglia has not yet been compared. Thus, in this study, we examined the fluorescence intensity of CD11b positive microglia and Glial Fibrillary Acidic Protein (GFAP) positive astrocytes and found that the fluorescent intensity was increased following incubation with PrP at 24 hours in a dose-dependent manner. We also observed an increase in interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) protein expression, which are initial inflammatory cytokines, in both PrP induced astrocytes and microglia. Furthermore, an increase MMP-1, 3 and 11 expressions in PrP induced astrocytes and microglia was observed by real time PCR. Our results demonstrated PrP induced activation of astrocytes and microglia respectively, which resulted in an increase in inflammatory cytokines and MMPs expression. These results provide the insight into the different sensitivities of glial cells to PrP.
authors.affiliation: Bio-Safety Research Institute, College of Veterinary Medicine, Chonbuk National University, Jeonju 561-156, Korea.
authors.forename: Kibbeum
authors.initials: K
authors.lastname: Song
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Na
authors.forename: Myung-Hoon
authors.initials: MH
authors.lastname: Oh
authors.forename: Sokho
authors.initials: S
authors.lastname: Kim
authors.forename: Young-Ha
authors.initials: YH
authors.lastname: Kim
authors.forename: Byung-Yong
authors.initials: BY
authors.lastname: Park
authors.forename: Gi-Wook
authors.initials: GW
authors.lastname: Shin
authors.forename: Bumseok
authors.initials: B
authors.lastname: Kim
authors.forename: Myungjo
authors.initials: M
authors.lastname: You
authors.forename: Jungkee
authors.initials: J
authors.lastname: Kwon
keywords: Astrocytes; Matrix metalloproteinase; Microglia; Prion peptide