title: Nucleotide sequences and stability of a Nicotiana nuclear DNA segment possessing autonomously replicating ability in yeast.
abstract: The nucleotide sequence of a tobacco (Nicotiana tabacum) chromosomal DNA segment(t3-ars) capable of replication in yeast (ars: autonomously replicating sequences) is presented. The subcloned region (618 bp) contained 11 bp consensus (5' A/TTTTATPuTTTA/T 3') essential for several yeast ars, and 73% A and T. Unique 70 bp repetitive sequences resided next to this sequence. Thirty-two bp AT repeats were also seen in the neighbourhood of the repetitive sequence. The hybrid plasmid containing t3-ars was mitotically stabilized by the help of yeast centromere (CEN4).
authors.affiliation: Institute of Biological Sciences, University of Tsukuba, Sakura-mura, 305, Ibaraki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ohtani
authors.forename: S
authors.initials: S
authors.lastname: Kiyokawa
authors.forename: T
authors.initials: T
authors.lastname: Ohgawara
authors.forename: H
authors.initials: H
authors.lastname: Harada
authors.forename: H
authors.initials: H
authors.lastname: Uchimiya