title: Insulin signaling in skeletal muscle of HIV-infected patients in response to endurance and strength training.
abstract: Human immunodeficiency virus (HIV)-infected patients with lipodystrophy have decreased insulin-stimulated glucose uptake. Both endurance and resistance training improve insulin-stimulated glucose uptake in skeletal muscle of HIV-infected patients, but the mechanisms are unknown. This study aims to identify the molecular pathways involved in the beneficial effects of training on insulin-stimulated glucose uptake in skeletal muscle of HIV-infected patients. Eighteen sedentary male HIV-infected patients underwent a 16 week supervised training intervention, either resistance or strength training. Euglycemic-hyperinsulinemic clamps with muscle biopsies were performed before and after the training interventions. Fifteen age- and body mass index (BMI)-matched HIV-negative men served as a sedentary baseline group. Phosphorylation and total protein expression of insulin signaling molecules as well as glycogen synthase (GS) activity were analyzed in skeletal muscle biopsies in relation to insulin stimulation before and after training. HIV-infected patients had reduced basal and insulin-stimulated GS activity (%fractional velocity, [FV]) as well as impaired insulin-stimulated Akt(thr308) phosphorylation. Despite improving insulin-stimulated glucose uptake, neither endurance nor strength training changed the phosphorylation status of insulin signaling proteins or affected GS activity. However; endurance training markedly increased the total Akt protein expression, and both training modalities increased hexokinase II (HKII) protein. HIV-infected patients with lipodystrophy have decreased insulin-stimulated glucose uptake in skeletal muscle and defects in insulin-stimulated phosphorylation of Akt(thr308). Endurance and strength training increase insulin-stimulated glucose uptake in these patients, and the muscular training adaptation is associated with improved capacity for phosphorylation of glucose by HKII, rather than changes in markers of insulin signaling to glucose uptake or glycogen synthesis.
authors.affiliation: Department of Infectious Diseases, Centre of Inflammation and Metabolism, Rigshospitalet Copenhagen, Denmark.
authors.forename: Christa
authors.initials: C
authors.lastname: Broholm
authors.forename: Neha
authors.initials: N
authors.lastname: Mathur
authors.forename: Thine
authors.initials: T
authors.lastname: Hvid
authors.forename: Thomas Sahl
authors.initials: TS
authors.lastname: Grøndahl
authors.forename: Christian
authors.initials: C
authors.lastname: Frøsig
authors.forename: Bente Klarlund
authors.initials: BK
authors.lastname: Pedersen
authors.forename: Birgitte
authors.initials: B
authors.lastname: Lindegaard
keywords: HIV; insulin signaling; lipodystrophy; skeletal muscle; training