title: N-acetylglucosaminidase, myeloperoxidase and vascular endothelial growth factor serum levels in breast cancer patients.
abstract: Inflammatory cells surround breast carcinomas and may act promoting tumor development or stimulating anti-tumor immunity. N-acetylglucosaminidase (NAG) has been employed to detect macrophage accumulation/activation. Myeloperoxidase (MPO) is considered a marker for neutrophils activity/accumulation. Vascular Endothelial Growth Factor (VEGF) is as strong pro-angiogenic cytokine. The aim of this study was to measure the systemic inflammatory response by measuring serum levels of NAG, MPO and VEGF in women diagnosed with breast cancer and associate this response to the peritumoral inflammatory infiltrate and to prognostic factors. Serum samples obtained from women with no evidence of disease (n=31) and with breast cancer (n=68) were analyzed for the activities of NAG, MPO and VEGF by enzymatic assay. Serum levels of NAG and VEGF were higher in healthy volunteers (P<0.0001) and serum levels of MPO were higher in patients with breast cancer (P=0.002). Serum levels of NAG were positively correlated to serum levels of MPO and VEGF (P<0.0001 and P=0.0012, respectively) and MPO and VEGF serum levels had also a positive correlation (P=0.0018). The inflammatory infiltrate was not associated to serum levels of the inflammatory markers, and higher levels of MPO were associated to lymphovascular invasion negativity (P=0.0175).
authors.affiliation: Department of Gynecology and Obstetrics, Faculty of Medicine, UNESP, Universidade Estadual Paulista "Julio de Mesquita Filho", Distrito de Rubião Júnior s/n(o) 18, 618-970 Botucatu, São Paulo, Brazil. Electronic address: budcoelho@gmail.com.
authors.forename: Bertha Andrade
authors.initials: BA
authors.lastname: Coelho
authors.affiliation: Department of Gynecology and Obstetrics, Faculty of Medicine, UFMG, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Andrezza Vilaça
authors.initials: AV
authors.lastname: Belo
authors.affiliation: Department of Physiology and Pharmacology, UFMG, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Sílvia Passos
authors.initials: SP
authors.lastname: Andrade
authors.affiliation: Department of Gynecology and Obstetrics, Faculty of Medicine, UFMG, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Washington Cançado
authors.initials: WC
authors.lastname: Amorim
authors.affiliation: Department of Gynecology and Obstetrics, Faculty of Medicine, UNESP, Universidade Estadual Paulista "Julio de Mesquita Filho", Distrito de Rubião Júnior s/n(o) 18, 618-970 Botucatu, São Paulo, Brazil.
authors.forename: Gilberto
authors.initials: G
authors.lastname: Uemura
authors.affiliation: Department of Gynecology and Obstetrics, Faculty of Medicine, UNESP, Universidade Estadual Paulista "Julio de Mesquita Filho", Distrito de Rubião Júnior s/n(o) 18, 618-970 Botucatu, São Paulo, Brazil; Department of Gynecology and Obstetrics, Faculty of Medicine, UFMG, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Agnaldo Lopes
authors.initials: AL
authors.lastname: da Silva Filho
mesh_terms: D000118:Acetylglucosaminidase / Q000097:blood*; D000293:Adolescent; D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000097:blood* / Q000276:immunology* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood; D008875:Middle Aged; D009195:Peroxidase / Q000097:blood*; D011379:Prognosis; D011446:Prospective Studies; D011795:Surveys and Questionnaires; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*; D055815:Young Adult
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D009195:Peroxidase; D000118:Acetylglucosaminidase
keywords: Myeloperoxidase (MPO); N-acetylglucosaminidase (NAG); Vascular Endothelial Growth Factor (VEGF)