title: 1H NMR-based metabolomics methods for chemical genomics experiments.
abstract: Metabolomics and chemical genomics studies can each provide unique insights into plant biology. Although a variety of analytical techniques can be used for the interrogation of plant systems, nuclear magnetic resonance (NMR) provides unbiased characterization of abundant metabolites. An example methodology is provided for probing the metabolism of Arabidopsis thaliana in a chemical genomics experiment including methods for tissue treatment, tissue collection, metabolite extraction, and methods to minimize variance in biological and technical sample replicates. Additionally, considerations and methods for data analysis, including multivariate statistics, univariate statistics, and data interpretation are included. The process is illustrated by examining the metabolic effects of chemical treatment of Arabidopsis with Sortin 1, also known as vacuolar protein sorting inhibitor 1. Sortin 1 was applied to Arabidopsis seedlings to examine metabolic effects in a chemical genomics experiment and to demonstrate the utility of metabolomics in conjunction with other "omics" techniques.
authors.affiliation: Department of Chemistry, University of California, Riverside, Riverside, CA, USA.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Orr
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Barding
authors.forename: Christiana E
authors.initials: CE
authors.lastname: Tolley
authors.forename: Glenn R
authors.initials: GR
authors.lastname: Hicks
authors.forename: Natasha V
authors.initials: NV
authors.lastname: Raikhel
authors.forename: Cynthia K
authors.initials: CK
authors.lastname: Larive
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism; D003627:Data Interpretation, Statistical; D018745:Genome, Plant; D023281:Genomics; D007192:Indenes / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D021381:Protein Transport; D011725:Pyridines / Q000494:pharmacology; D036226:Seedlings / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D007192:Indenes; D011725:Pyridines; C558636:sortin1 compound