title: Activation of primary sensory neurons by the topical application of capsaicin on the epidermis of a re-innervated organotypic human skin model.
abstract: Using an ex vivo skin-nerve preparation, skin and nerve cells were reconstituted into a single unit and maintained in a nutrient medium bath until required experimentally. Our objective was to use the epidermis as a relay for the induction of an electric current to the neurons following the topical application of capsaicin on the skin epidermis of the skin explant, an agonist of the TRPV1 channel implicated in pruritus and pain. After 10-20 days of coculture to form the re-innervated skin model, we applied a solution of capsaicin directly on the epidermis of the skin explant (4 μm). The resulting current was recorded using a path-clamp technique on the neuronal fibres. Following the topical application of capsaicin, spontaneous activity was triggered, as characterised by repetitive spikes with periods of 125, 225 or 275 ms. This study demonstrates that the skin explant and nerve cells preparation may receive stimuli and be used to screen molecules or to study signal transmission.
authors.affiliation: Laboratory of Neurosciences of Brest, EA4685, Faculty of Medicine and Health Sciences, University of Brest, Brest, France; BASF Beauty Care Solutions, Pulnoy, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Lebonvallet
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Pennec
authors.forename: Christelle
authors.initials: C
authors.lastname: Le Gall-Ianotto
authors.forename: Jérémy
authors.initials: J
authors.lastname: Chéret
authors.forename: Christine
authors.initials: C
authors.lastname: Jeanmaire
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Carré
authors.forename: Gilles
authors.initials: G
authors.lastname: Pauly
authors.forename: Laurent
authors.initials: L
authors.lastname: Misery
mesh_terms: D000287:Administration, Topical; D000818:Animals; D002211:Capsaicin / Q000008:administration & dosage*; D018920:Coculture Techniques; D004817:Epidermis / Q000187:drug effects* / Q000294:innervation*; D006801:Humans; D008959:Models, Neurological; D009924:Organ Culture Techniques; D051381:Rats; D011984:Sensory Receptor Cells / Q000187:drug effects* / Q000502:physiology
chemical_list: D002211:Capsaicin
keywords: human; neuron; skin