title: Positive correlation of STAT1 and miR-146a with anemia in patients with systemic lupus erythematosus.
abstract: OBJECTIVE
  Anemia is one of the most common hematological manifestations in SLE patients, occurring in about 50% of active cases. STAT1 is a critical signaling molecule required for the production of type-1 interferon (I-IFN), CCL2, and CXCL10, all of which are upregulated in SLE. Overexpression of STAT1 has been described to be involved in anemia in animal models. The aim of this study is to analyze how these components are involved in SLE-associated anemia.
  
  
  METHODS
  Blood samples were collected from 39 healthy donors and 101 SLE patients fulfilling ACR criteria. Samples were collected from a total of 180 visits (58 patients had 2 or more visits) of which 52 visits included a diagnosis of anemia. Healthy donors had only single visit. Total RNA, isolated from leukocytes, was analyzed by Taqman qPCR. Relative expression levels of I-IFN signature genes, chemokines, and miR-146a were determined by the ΔΔCT method. Results were correlated with clinical data and analyzed by the Wilcoxon/Kruskal-Wallis test and Fisher's exact test.
  
  
  RESULTS
  Significant increases in IFN score (p < 0.0001), STAT1 (p < 0.0001), miR-146a (p < 0.0005), CCL2 (p = 0.0047), and CXCL10 (p = 0.017), as well as a significant decrease in pri-miR-146a (p = 0.0002), were detected in the anemic SLE patient visits (n = 52) compared to non-anemic SLE visits (n = 128). Regardless of disease activity, lupus nephritis, or race, anemic SLE patients displayed significantly elevated levels of STAT1 and miR-146a compared to non-anemic SLE patients.
  
  
  CONCLUSIONS
  STAT1 and miR-146a may be upregulated during inflammation and via proinflammatory cytokines and chemokines in SLE. Prolonged upregulation of STAT1 and miR-146a appears to play an important role in anemia in SLE patients.
authors.affiliation: Department of Oral Biology, University of Florida, 1395 Center Drive, Gainesville, FL, 32610-0424, USA.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Dominguez-Gutierrez
authors.forename: Angela
authors.initials: A
authors.lastname: Ceribelli
authors.forename: Minoru
authors.initials: M
authors.lastname: Satoh
authors.forename: Eric S
authors.initials: ES
authors.lastname: Sobel
authors.forename: Westley H
authors.initials: WH
authors.lastname: Reeves
authors.forename: Edward K L
authors.initials: EK
authors.lastname: Chan
mesh_terms: D000328:Adult; D000367:Age Factors; D000740:Anemia / Q000150:complications / Q000175:diagnosis / Q000209:etiology*; D015415:Biomarkers; D018932:Chemokine CCL2 / Q000235:genetics; D054357:Chemokine CXCL10 / Q000235:genetics; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000150:complications* / Q000175:diagnosis / Q000235:genetics* / Q000628:therapy; D008181:Lupus Nephritis / Q000150:complications; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D044382:Population Groups / Q000235:genetics; D050794:STAT1 Transcription Factor / Q000235:genetics*; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D015415:Biomarkers; D018932:Chemokine CCL2; D054357:Chemokine CXCL10; C542542:MIRN146 microRNA, human; D035683:MicroRNAs; D050794:STAT1 Transcription Factor