title: The genetic structure of the A mating-type locus of Lentinula edodes.
abstract: The Shiitake mushroom, Lentinula edodes (Berk.) Pegler is a tetrapolar basidiomycete with two unlinked mating-type loci, commonly called the A and B loci. Identifying the mating-types in shiitake is important for enhancing the breeding and cultivation of this economically-important edible mushroom. Here, we identified the A mating-type locus from the first draft genome sequence of L. edodes and characterized multiple alleles from different monokaryotic strains. Two intron-length polymorphism markers were developed to facilitate rapid molecular determination of A mating-type. L. edodes sequences were compared with those of known tetrapolar and bipolar basidiomycete species. The A mating-type genes are conserved at the homeodomain region across the order Agaricales. However, we observed unique genomic organization of the locus in L. edodes which exhibits atypical gene order and multiple repetitive elements around its A locus. To our knowledge, this is the first known exception among Homobasidiomycetes, in which the mitochondrial intermediate peptidase (mip) gene is not closely linked to A locus.
authors.affiliation: Biology Programme, School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
authors.forename: Chun Hang
authors.initials: CH
authors.lastname: Au
authors.affiliation: Biology Programme, School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
authors.forename: Man Chun
authors.initials: MC
authors.lastname: Wong
authors.affiliation: Edible Fungi Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Bao
authors.affiliation: Edible Fungi Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.
authors.forename: Meiyan
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Edible Fungi Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Song
authors.affiliation: Edible Fungi Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Song
authors.affiliation: Biology Programme, School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
authors.forename: Patrick Tik Wan
authors.initials: PT
authors.lastname: Law
authors.affiliation: Division of Molecular Wood Biotechnology and Technical Mycology, Büsgen-Institute, University of Göttingen, Büsgenweg 2, 37077 Göttingen, Germany.
authors.forename: Ursula
authors.initials: U
authors.lastname: Kües
authors.affiliation: Biology Programme, School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, China. Electronic address: hoishankwan@cuhk.edu.hk.
authors.forename: Hoi Shan
authors.initials: HS
authors.lastname: Kwan
mesh_terms: D002874:Chromosome Mapping; D023061:Gene Order; D005800:Genes, Fungal; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D040641:Quantitative Trait Loci; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D020942:Shiitake Mushrooms / Q000235:genetics*
keywords: BLAST; Basic Local Alignment Search Tool; Di; HD; HD1; HD2; Homeodomain; ILP; Lentinula edodes; MIP; Mating-type; Mushroom; NLS; TSS; Tetrapolar; beta-flanking gene; dimerization motif; homeodomain; homeodomain type 1 transcription factor; homeodomain type 2 transcription factor; intron-length polymorphism markers; mitochondrial intermediate peptidase; nuclear localization signal; transcription start site; β-fg