title: Gene expression analysis using DNA microarray in HK-2 human proximal tubular cells treated with cadmium.
abstract: We examined the alteration of gene expression in HK-2 human proximal tubular cells exposed to cadmium (Cd) using DNA microarray analysis. Cd increased the expression of 30 genes, including 7 genes coding for heat shock proteins, more than 2.0-fold and decreased the expression of 21 genes, including transcription-related genes, such as AP2B1, HOXA7, HOXA9 and TCEB2, less than 0.5-fold prior to the appearance of cytotoxicity in HK-2 cells.
authors.affiliation: Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University.
authors.forename: Jin-Yong
authors.initials: JY
authors.lastname: Lee
authors.forename: Maki
authors.initials: M
authors.lastname: Tokumoto
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Fujiwara
authors.forename: Masahiko
authors.initials: M
authors.lastname: Satoh
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000235:genetics; D019187:Cadmium Compounds / Q000633:toxicity*; D027682:Cation Transport Proteins / Q000235:genetics; D002470:Cell Survival / Q000235:genetics*; D002478:Cells, Cultured; D015536:Down-Regulation / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects* / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D051547:Heme Oxygenase-1 / Q000235:genetics; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D007687:Kidney Tubules, Proximal / Q000166:cytology* / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis; D013997:Time Factors; D059467:Transcriptome / Q000187:drug effects* / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; C414431:BAG3 protein, human; D019187:Cadmium Compounds; D027682:Cation Transport Proteins; C493901:HPS6 protein, human; D018840:HSP70 Heat-Shock Proteins; C490786:HSPA1A protein, human; D047908:Intracellular Signaling Peptides and Proteins; C403517:SLC39A1 protein, human; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1