title: Seminal PDC-109 protein vis-à-vis cholesterol content and freezability of buffalo spermatozoa.
abstract: Advancements in reproductive technologies have shown seminal plasma (SP) as a nutritive-protective medium for spermatozoa metabolism, function and transport. At the same time quality variables and thus freezability of spermatozoa are influenced by SP proteins originating from male reproductive tract. One such protein, viz. PDC-109 is reported to influence freezability of spermatozoa in cattle. Thus the present investigation was designed to evaluate effect of seminal PDC-109 protein concentration on post-thaw cholesterol content and semen quality variables (SQP) as an indicator of membrane integrity and freezability, respectively of buffalo spermatozoa. Ejaculates (n=42) selected on the basis of mass activity and individual motility were divided into three parts, first part for SP proteins isolation, second for cholesterol estimation and third part was cryo-preserved to evaluate freezability based on post-thaw SQP, viz. individual progressive motility, viability and acrosome integrity of spermatozoa. A total of 28 (66.7%) and 14 (33.3%) ejaculates from four bulls were found as freezable or non-freezable, respectively. Though total seminal plasma protein (TSPP) concentration was found similar in freezable and non-freezable ejaculates, the heparin binding proteins (HBP) content in non-freezable semen was greater (P<0.01) than freezable ejaculates. There was a similar trend for the PDC-109 protein content in respective ejaculates. Cholesterol content of spermatozoa and SQP were greater (P<0.05 and 0.01, respectively) in freezable as compared to non-freezable ejaculates of each bull at post-thaw stage. This study showed that concentrations of HBP and PDC-109 in non-freezable semen might be responsible for greater cryo-damage reflecting in poor freezability of buffalo spermatozoa.
authors.affiliation: Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors.forename: Mahak
authors.initials: M
authors.lastname: Singh
authors.affiliation: Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Ghosh
authors.affiliation: Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors.forename: J K
authors.initials: JK
authors.lastname: Prasad
authors.affiliation: Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors.forename: Anuj
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors.forename: R P
authors.initials: RP
authors.lastname: Tripathi
authors.affiliation: Division of Animal Biochemistry, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Bhure
authors.affiliation: Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar 243122, India. Electronic address: sangnee15@gmail.com.
authors.forename: N
authors.initials: N
authors.lastname: Srivastava
mesh_terms: D000177:Acrosome / Q000502:physiology; D000818:Animals; D002020:Buffaloes; D002784:Cholesterol / Q000032:analysis*; D015925:Cryopreservation / Q000592:standards / Q000662:veterinary*; D008297:Male; D012661:Semen / Q000737:chemistry*; D055101:Semen Analysis; D012662:Semen Preservation / Q000592:standards / Q000662:veterinary*; D029589:Seminal Plasma Proteins / Q000032:analysis* / Q000378:metabolism; D013081:Sperm Motility; D013094:Spermatozoa / Q000737:chemistry
chemical_list: D029589:Seminal Plasma Proteins; D002784:Cholesterol
keywords: Buffalo bull; Cryopreservation; Freezability; Heparin binding proteins; PDC-109 protein; Spermatozoa