title: DPPrimer - A Degenerate PCR Primer Design Tool.
abstract: Designed degenerate primers unlike conventional primers are superior in matching and amplification of large number of genes, from related gene families. DPPrimer tool was designed to predict primers for PCR amplification of homologous gene from related or diverse plant species. The key features of this tool include platform independence and user friendliness in primer design. Embedded features such as search for functional domains, similarity score selection and phylogebetic tree further enhance the user friendliness of DPPrimer tool. Performance of DPPrimer tool was evaluated by successful PCR amplification of ADP-glucose phosphorylase genes from wheat, barley and rice.
  
  
  BACKGROUND
  DPPrimer is freely accessible at http://202.141.12.147/DGEN_tool/index.html.
authors.affiliation: National Bureau of Plant Genetic Resources, Pusa campus, New Delhi-12.
authors.forename: Shachi
authors.initials: S
authors.lastname: Gahoi
authors.forename: L
authors.initials: L
authors.lastname: Arya
authors.forename: Rai
authors.initials: R
authors.lastname: Anil
authors.forename: Ss
authors.initials: S
authors.lastname: Marla
keywords: Degeneracy; PCR; Primer design