title: Enzymatic hydrolysis of autohydrolyzed wheat straw followed by refining to produce fermentable sugars.
abstract: Wheat straw was pretreated using an autohydrolysis process with different temperatures (160-200 °C) and times (10-20 min) in order to allow the recovery of hemicellulose in the filtrate and help open up the structure of the biomass for improved accessibility of enzymes during enzymatic hydrolysis. Autohydrolysis at 190 °C for 10 min provided the highest overall sugar (12.2/100g raw wheat straw) in the autohydrolysis filtrate and recovered 62.3% of solid residue. Before enzymatic hydrolysis, the pulps obtained from each pretreatment condition were subjected to a refining post-treatment to improve enzyme accessibility. Enzymatic hydrolysis was performed for all the pretreated solids with and without refining post-treatment at the enzyme loadings of 4 and 10 FPU/g oven dry substrate for 96 h. A total of 30.4 g sugars can be recovered from 100g wheat straw at 180 °C for 20 min with 4 FPU/g enzyme charge.
authors.affiliation: Department of Forest Industry Engineering, Bursa Technical University, 16200 Bursa, Turkey. Electronic address: murat.ertas@btu.edu.tr.
authors.forename: Murat
authors.initials: M
authors.lastname: Ertas
authors.affiliation: Department of Forest Biomaterials, North Carolina State University, Campus Box 8005, Raleigh, NC 27695-8005, USA.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Han
authors.affiliation: Department of Forest Biomaterials, North Carolina State University, Campus Box 8005, Raleigh, NC 27695-8005, USA.
authors.forename: Hasan
authors.initials: H
authors.lastname: Jameel
authors.affiliation: Department of Forest Biomaterials, North Carolina State University, Campus Box 8005, Raleigh, NC 27695-8005, USA.
authors.forename: Hou-min
authors.initials: HM
authors.lastname: Chang
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000096:biosynthesis*; D002480:Cellulase / Q000378:metabolism*; D005285:Fermentation; D005374:Filtration; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D014908:Triticum / Q000378:metabolism*; D014866:Waste Products
chemical_list: D002241:Carbohydrates; D014866:Waste Products; D002480:Cellulase
keywords: Autohydrolysis; Bioethanol; Enzymatic hydrolysis; Fermentable sugar; Wheat straw