title: Microfluidic perfusion culture.
abstract: Microfluidic perfusion culture is a novel technique to culture animal cells in a small-scale microchamber with medium perfusion. Polydimethylsiloxane (PDMS) is the most popular material to fabricate a microfluidic perfusion culture chip. Photolithography and replica molding techniques are generally used for fabrication of a microfluidic perfusion culture chip. Pressure-driven perfusion culture system is convenient technique to carry out the perfusion culture of animal cells in a microfluidic device. Here, we describe a general theory on microfluid network design, microfabrication technique, and experimental technique for pressure-driven perfusion culture in an 8 × 8 microchamber array on a glass slide-sized microchip made out of PDMS.
authors.affiliation: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Hattori
authors.forename: Shinji
authors.initials: S
authors.lastname: Sugiura
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kanamori
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D004129:Dimethylpolysiloxanes; D004867:Equipment Design; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D010477:Perfusion / Q000295:instrumentation; D011312:Pressure
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon