title: BTG2 overexpression increases the radiosensitivity of breast cancer cells in vitro and in vivo.
abstract: Antiproliferative gene B-cell translocation gene, member 2 (BTG2) is a member of the BTG/TOB antiproliferative gene family. In this study, we investigated the effect of BTG2 gene overexpression on the radiosensitivity of breast cancer cells in vitro and in vivo. Results show that in human breast cancer cell line MCF-7 stably overexpressing BTG2 gene, cell sensitivity to ionizing radiation increased. The MCF-7-BTG2 cells were more susceptible to radiation-caused apoptosis with decreased cyclin B1, cyclin D1, Ku70, FEN-1, and XRCC1 protein expression as well as increased BAX protein expression. The findings indicate for the first time that BTG2 can improve the radiosensitivity of breast cancer cells by affecting cell cycle distribution, enhancing radiation-induced apoptosis, and inhibiting DNA repair-related protein expression.
authors.affiliation: Department of Radiation Oncology, Shandong Tumor Hospital and Institute, Jinan, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Hu
authors.forename: Ligang
authors.initials: L
authors.lastname: Xing
authors.forename: Yang
authors.initials: Y
authors.lastname: Jiao
authors.forename: Jiaying
authors.initials: J
authors.lastname: Xu
authors.forename: Xingwu
authors.initials: X
authors.lastname: Wang
authors.forename: Anqin
authors.initials: A
authors.lastname: Han
authors.forename: Jinming
authors.initials: J
authors.lastname: Yu
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear / Q000378:metabolism; D017209:Apoptosis / Q000528:radiation effects; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D002453:Cell Cycle / Q000528:radiation effects; D056744:Cyclin B1 / Q000378:metabolism; D019938:Cyclin D1 / Q000378:metabolism; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D005260:Female; D045585:Flap Endonucleases / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism*; D000072200:Ku Autoantigen; D061986:MCF-7 Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011836:Radiation Tolerance; D013997:Time Factors; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D000076105:X-ray Repair Cross Complementing Protein 1; D023041:Xenograft Model Antitumor Assays; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D034961:Antigens, Nuclear; C494826:BAX protein, human; C534699:CCNB1 protein, human; C530104:CCND1 protein, human; D056744:Cyclin B1; D004268:DNA-Binding Proteins; D017874:Immediate-Early Proteins; D025521:Tumor Suppressor Proteins; D000076105:X-ray Repair Cross Complementing Protein 1; C000619371:XRCC1 protein, human; C000619352:Xrcc1 protein, mouse; D051028:bcl-2-Associated X Protein; D019938:Cyclin D1; C070114:BTG2 protein, human; D045585:Flap Endonucleases; C468317:FEN1 protein, human; C000606509:Xrcc6 protein, human; C000606510:Xrcc6 protein, mouse; D000072200:Ku Autoantigen