title: Modulating enzyme activity using ionic liquids or surfactants.
abstract: One of the important strategies for modulating enzyme activity is the use of additives to affect their microenvironment and subsequently make them suitable for use in different industrial processes. Ionic liquids (ILs) have been investigated extensively in recent years as such additives. They are a class of solvents with peculiar properties and a "green" reputation in comparison to classical organic solvents. ILs as co-solvents in aqueous systems have an effect on substrate solubility, enzyme structure and on enzyme-water interactions. These effects can lead to higher reaction yields, improved selectivity, and changes in substrate specificity, and thus there is great potential for IL incorporation in biocatalysis. The use of surfactants, which are usually denaturating agents, as additives in enzymatic reactions is less reviewed in recent years. However, interesting modulations in enzyme activity in their presence have been reported. In the case of surfactants there is a more pronounced effect on the enzyme structure, as can be observed in a number of crystal structures obtained in their presence. For each additive and enzymatic process, a specific optimization process is needed and there is no one-fits-all solution. Combining ILs and surfactants in either mixed micelles or water-in-IL microemulsions for use in enzymatic reaction systems is a promising direction which may further expand the range of enzyme applications in industrial processes. While many reviews exist on the use of ILs in biocatalysis, the present review centers on systems in which ILs or surfactants were able to modulate and improve the natural activity of enzymes in aqueous systems.
authors.affiliation: Department of Biotechnology and Food Engineering, Technion - Israel Institute of Technology, Haifa, 32000, Israel.
authors.forename: Mor
authors.initials: M
authors.lastname: Goldfeder
authors.forename: Ayelet
authors.initials: A
authors.lastname: Fishman
mesh_terms: D020536:Enzyme Activators / Q000378:metabolism*; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism*; D052578:Ionic Liquids / Q000378:metabolism*; D011487:Protein Conformation; D012995:Solubility; D012997:Solvents; D013501:Surface-Active Agents / Q000378:metabolism*
chemical_list: D020536:Enzyme Activators; D004798:Enzymes; D052578:Ionic Liquids; D012997:Solvents; D013501:Surface-Active Agents