title: Effects of sulfate group in red seaweed polysaccharides on anticoagulant activity and cytotoxicity.
abstract: In this paper, the structural effects of two main red seaweed polysaccharides (agarose and carrageenan) and their sulfated derivatives on the anticoagulant activity and cytotoxicity were investigated. The substitution position rather than the substitution degree of sulfate groups shows the biggest impact on both the anticoagulant activity and the cell proliferation. Among them, C-2 of 3,6-anhydro-α-d-Galp is the most favorable position for substitution, whereas C-6 of β-d-Galp is the most disadvantageous. Moreover, the secondary structures of glycans also play a key role in biological activities. These demonstrations warrant that the red seaweed polysaccharides should be seriously considered in biomedical applications after carefully tailoring the sulfate groups.
authors.affiliation: Department of Biomedical Engineering, Jinan University, 510632 Guangzhou, PR China.
authors.forename: Wanai
authors.initials: W
authors.lastname: Liang
authors.forename: Xuan
authors.initials: X
authors.lastname: Mao
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Peng
authors.forename: Shunqing
authors.initials: S
authors.lastname: Tang
mesh_terms: D000925:Anticoagulants / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D049109:Cell Proliferation / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008970:Molecular Weight; D010314:Partial Thromboplastin Time; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D011517:Prothrombin Time; D012624:Seaweed / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013431:Sulfates / Q000737:chemistry*
chemical_list: D000925:Anticoagulants; D011134:Polysaccharides; D013431:Sulfates
keywords: Agarose; Anticoagulant; Carrageenan; Cytotoxicity; Oligosaccharide; Sulfation