title: Determination of sec-O-glucosylhamaudol in rat plasma by gradient elution liquid chromatography-mass spectrometry.
abstract: Sec-O-glucosylhamaudol is one of the major bioactive compounds of the Saposhnikoviae Radix. A simple and selective liquid chromatography-mass spectrometry (LC-MS) method for determination of sec-O-glucosylhamaudol in rat plasma was developed. After addition of carbamazepine as internal standard (IS), protein precipitation with acetonitrile-methanol (9:1, v/v) was used as sample preparation. Chromatographic separation was achieved on a Zorbax SB-C18 (2.1mm×150mm, 5μm) column with acetonitrile-0.1% formic acid in water as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selective ion monitoring (SIM) mode was used for quantification using target fragment ions m/z 439 for sec-O-glucosylhamaudol and m/z 237 for the IS. Calibration plots were linear over the range of 50-8000ng/mL for sec-O-glucosylhamaudol in rat plasma. Mean recovery of sec-O-glucosylhamaudol in plasma was in the range of 74.8-83.7%. Intra-day and inter-day precision were both <15%. This method was successfully applied in pharmacokinetic study after intravenous administration of 2.5mg/kg sec-O-glucosylhamaudol in rats.
authors.affiliation: Laboratory Animal Centre of Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Wen
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Chongliang
authors.initials: C
authors.lastname: Lin
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Cai
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Jianshe
authors.initials: J
authors.lastname: Ma
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou 325035, China. Electronic address: lankywang@foxmail.com.
authors.forename: Xianqin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D002220:Carbamazepine; D002853:Chromatography, Liquid / Q000379:methods*; D002867:Chromones / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D016014:Linear Models; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D002867:Chromones; D002220:Carbamazepine
keywords: LC–MS; Pharmacokinetics; Rat plasma; Sec-O-glucosylhamaudol