title: A new bead-based human platelet antigen antibodies detection assay versus the monoclonal antibody immobilization of platelet antigens assay.
abstract: BACKGROUND
  The performance of a newly developed Luminex bead-based platelet (PLT) antibody detection method (PAKLx) was compared with the monoclonal antibody immobilization of PLT antigens (MAIPA) assay and the LifeScreen Deluxe Luminex bead-based HLA Class I antibody detection method (LMX).
  
  
  METHODS
  Six sera containing anti-human PLT antigen (HPA)-1a (n=2), HPA-1b, HPA-2b, HPA-3a, or HPA-5b were tested in titration. A total of 194 sera, including HPA-1a, -1b, -2a, -2b, -3a, -5a, and -5b antibodies with or without HLA antibodies (n=63); glycoprotein (GP) IV antibodies (n=1); PLT autoantibodies (n=3); HLA antibodies (n=45); and samples with no PLT-reactive antibodies (n=82), were tested in both assays.
  
  
  RESULTS
  Comparable levels of sensitivity were obtained for the MAIPA and PAKLx. The PAKLx showed four (6%) false-negative results in 67 sera with HPA or GP-reactive antibodies: anti-HPA-3a (n=1) or anti-HPA-5b (n=3). The PAKLx showed in 10 of the total 194 samples (5%) the presence of antibodies not detected by the MAIPA. This concerned broadly GP-reactive antibodies (n=7), anti-GPIIb/IIIa combined with anti-HPA-3a (n=1), anti-HPA-1a (borderline, n=1), and anti-GPIV (n=1). Testing 175 sera for anti-HLA Class I antibodies in the PAKLx and LMX showed four discrepant results: PAKLx negative and LMX positive, n=3 and n=1, respectively.
  
  
  CONCLUSIONS
  For the vast majority of the specimens tested (93%) the results of the PAKLx were in concordance with the MAIPA. The PAKLx is a fast, easy to perform, and sensitive PLT antibody screening method.
authors.affiliation: Sanquin Diagnostic Services, Amsterdam, The Netherlands.
authors.forename: Leendert
authors.initials: L
authors.lastname: Porcelijn
authors.forename: Elly
authors.initials: E
authors.lastname: Huiskes
authors.forename: Ilona
authors.initials: I
authors.lastname: Comijs-van Osselen
authors.forename: Aniska
authors.initials: A
authors.lastname: Chhatta
authors.forename: Vipul
authors.initials: V
authors.lastname: Rathore
authors.forename: Matthew
authors.initials: M
authors.lastname: Meyers
authors.forename: Masja
authors.initials: M
authors.lastname: de Haas
mesh_terms: D000906:Antibodies / Q000032:analysis* / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology*; D016824:Antigens, Human Platelet / Q000276:immunology*; D001681:Biological Assay / Q000379:methods*; D006801:Humans; D039661:Integrin beta3
chemical_list: C115212:2a alloantigen, human; C044561:3a alloantigen, human; C071092:5a alloantigen, human; C055973:5b alloantigen, human; D000906:Antibodies; D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; C061248:ITGB3 protein, human; D039661:Integrin beta3; C083451:human platelet antigen 1b