title: Subculture-induced protein synthesis in tissue cultures of Glycine max and Phaseolus vulgaris.
abstract: The effects of subculture of tissue cultures on the levels of certain mRNAs have been investigated, and the action of cytokinins on the disposition of certain mRNAs between possible non-translating and translating pools has been determined. mRNA preparations were assayed by cell free translation with message-dependent reticulocyte lysate and the in vitro products resolved by polyacrylamide gel electrophoresis. Subculture of the cells caused a rapid stimulation of polysome formation. It also increased the translatable levels of a small group of mRNAs, one of which was present in both bean and soybean cultures. Cytokinins caused a slight increase in polysome levels after subculture, but had no effect on the levels of particular mRNAs, nor on the distribution of mRNAs between a non-translating and translating pool, nor on polysome levels in the absence of subculture.
authors.affiliation: Department of Biochemistry, University of Cambridge, CB2 1QW, Cambridge, UK.
authors.forename: M
authors.initials: M
authors.lastname: Bevan
authors.forename: D H
authors.initials: DH
authors.lastname: Northcote