title: Identification of a CYP3A-like gene and CYPs mRNA expression modulation following exposure to benzo[a]pyrene in the bivalve mollusk Chlamys farreri.
abstract: In this study, we isolated a CYP3A-like gene from ovary of the scallop (Chlamys farreri). High levels of CYP3A-like gene expression occur in the digestive gland and gonad, which suggested their role in the metabolism of steroids and xenobiotics. Scallops were exposed to a polycyclic aromatic hydrocarbons (PAHs), benzo[a]pyrene (B[a]P) for 10 days. The CYP4 and CYP3A-like gene can be up-regulated by B[a]P in a dose-dependent manner after 10 days exposure. But no induction of the CYP3A-like was observed in 10 μg/L B[a]P group. The CYP1A-like expression can only be induced by 0.025 μg/L B[a]P. 0.5 and 10 μg/L B[a]P caused significant DNA damage and 10 μg/L B[a]P can also lead to oxidative damage. These results demonstrate that the mollusk CYPs can be modulated by environmental pollutant, and the blocked induction of CYP3A-like and CYP1A-like expression probably results from the high genotoxicity and oxidative damage partly.
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China, Yushan Road 5, Qingdao 266003, PR China.
authors.forename: Shuangmei
authors.initials: S
authors.lastname: Tian
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China, Yushan Road 5, Qingdao 266003, PR China. Electronic address: panlq@ouc.edu.cn.
authors.forename: Luqing
authors.initials: L
authors.lastname: Pan
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China, Yushan Road 5, Qingdao 266003, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D051544:Cytochrome P-450 CYP3A / Q000235:genetics* / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D008969:Molecular Sequence Data; D010053:Ovary / Q000201:enzymology; D018384:Oxidative Stress / Q000187:drug effects; D049897:Pectinidae / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics*; D016415:Sequence Alignment; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene; D051544:Cytochrome P-450 CYP3A
keywords: B[a]P; Benzo[a]pyrene; CYP3A-like; CYPs expression; Chlamys farreri; Ecotoxicology; PAHs; Pollution effects; Polycyclic aromatic hydrocarbons; mRNA