title: FRET-based dimeric aptamer probe for selective and sensitive Lup an 1 allergen detection.
abstract: A sensitive method for the rapid and sensitive detection of the anaphylactic food allergen Lup an 1 (β-conglutin) exploiting fluorescence resonance energy transfer (FRET) has been developed. A high affinity dimeric form of a truncated 11-mer aptamer against β-conglutin was used, with each monomeric aptamer being flanked by donor/acceptor moieties. The dimeric form in the absence of target yields fluorescence emission due to the FRET from the excited fluorophore to the proximal second fluorophore. However, upon addition of β-conglutin, the specific interaction induces a change in the bi-aptameric structure resulting in an increase in fluorescence emission. The method is highly specific and sensitive, with a detection limit of 150 pM, providing an effective tool for the direct detection of the toxic β-conglutin subunit in foodstuffs in just 1 min at room temperature.
authors.affiliation: Nanobiotechnology and Bioanalysis group, Department of Chemical Engineering, Universitat Rovira i Virgili, 43007, Tarragona, Spain.
authors.forename: T
authors.initials: T
authors.lastname: Mairal
authors.affiliation: Nanobiotechnology and Bioanalysis group, Department of Chemical Engineering, Universitat Rovira i Virgili, 43007, Tarragona, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Nadal
authors.affiliation: Nanobiotechnology and Bioanalysis group, Department of Chemical Engineering, Universitat Rovira i Virgili, 43007, Tarragona, Spain. Electronic address: marketa.svobodova@urv.cat.
authors.forename: M
authors.initials: M
authors.lastname: Svobodova
authors.affiliation: Nanobiotechnology and Bioanalysis group, Department of Chemical Engineering, Universitat Rovira i Virgili, 43007, Tarragona, Spain; Institució Catalana de Recerca I Estudis Avancats, Passeig Lluís Companys 23, 08010 Barcelona, Spain. Electronic address: ciara.osullivan@urv.cat.
authors.forename: C K
authors.initials: CK
authors.lastname: O'Sullivan
mesh_terms: D000485:Allergens / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods; D019281:Dimerization; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D057230:Limit of Detection; D031297:Lupinus / Q000737:chemistry*; D055314:Seed Storage Proteins / Q000032:analysis*
chemical_list: D000485:Allergens; D052157:Aptamers, Nucleotide; D055314:Seed Storage Proteins; C042457:conglutin protein, Lupinus angustifolius
keywords: 11mer; FRET; Lup an 1 allergen; β-conglutin aptamer