title: Electrochemical single-molecule detection in aqueous solution using self-aligned nanogap transducers.
abstract: Electrochemical detection of individual molecular tags in nanochannels may enable cost-effective, massively parallel analysis and diagnostics platforms. Here we demonstrate single-molecule detection of prototypical analytes in aqueous solution based on redox cycling in 40 nm nanogap transducers. These nanofluidic devices are fabricated using standard microfabrication techniques combined with a self-aligned approach that minimizes gap size and dead volume. We demonstrate the detection of three common redox mediators at physiological salt concentrations.
authors.affiliation: MESA+ Institute for Nanotechnology, University of Twente , PO Box 217, 7500 AE Enschede, The Netherlands.
authors.forename: Shuo
authors.initials: S
authors.lastname: Kang
authors.forename: Ab F
authors.initials: AF
authors.lastname: Nieuwenhuis
authors.forename: Klaus
authors.initials: K
authors.lastname: Mathwig
authors.forename: Dileep
authors.initials: D
authors.lastname: Mampallil
authors.forename: Serge G
authors.initials: SG
authors.lastname: Lemay
mesh_terms: D000327:Adsorption; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D044085:Microfluidics; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D055095:Optics and Photonics; D010084:Oxidation-Reduction; D013053:Spectrophotometry; D013499:Surface Properties; D014159:Transducers; D014867:Water / Q000737:chemistry*
chemical_list: D014867:Water