title: Preclinical evaluation of holmium-166 labeled anti-VEGF-A(Bevacizumab).
abstract: Radiolabeled antiangiogenic monoclonal antibodies are potential agents for targeted therapy in specific types of malignancies. In this study, (166)Ho-DOTA-Bevacizumab was used in biodistribution studies using single-photon emission computed tomography (SPECT) to acquire dosimetric aspects of the radiolabeled antibody in mice. The liver toxicity of the radiolabeled antibody was also determined using serum glutamic pyruvic transaminase, serum glutamic oxaloacetic transaminase and alkaline phosphatase assay 2-7 days post-injection. The SPECT biodistribution demonstrated a similar pattern as the other radiolabeled anti-vascular endothelial growth factor A (VEGF-A) immunoconjugates. (166)Ho-DOTA-Bevacizumab was revealed as a potential compound for therapy/imaging of VEGF-A expression in oncology.
authors.affiliation: Medical Physics Department, Tehran University of Medical Sciences (TUMS), Tehran, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Khorami-Moghadam
authors.forename: Bahram
authors.initials: B
authors.lastname: Bolouri
authors.forename: Amir Reza
authors.initials: AR
authors.lastname: Jalilian
authors.forename: Nariman Mosafa Ali
authors.initials: NM
authors.lastname: Bahrami-Samani
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Mazidi
authors.forename: Behrouz
authors.initials: B
authors.lastname: Alirezapour
mesh_terms: D020533:Angiogenesis Inhibitors / Q000493:pharmacokinetics*; D000818:Animals; D061067:Antibodies, Monoclonal, Humanized / Q000493:pharmacokinetics*; D000068258:Bevacizumab; D004353:Drug Evaluation, Preclinical; D006695:Holmium / Q000493:pharmacokinetics*; D051379:Mice; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D051381:Rats; D015899:Tomography, Emission-Computed, Single-Photon
chemical_list: D020533:Angiogenesis Inhibitors; D061067:Antibodies, Monoclonal, Humanized; D019275:Radiopharmaceuticals; D000068258:Bevacizumab; D006695:Holmium
keywords: bevacizumab; biodistribution; conjugation; holmium-166; radiolabeling