title: Enzymolysis-ultrasonic assisted extraction, chemical characteristics and bioactivities of polysaccharides from corn silk.
abstract: An enzymolysis-ultrasonic assisted extraction (EUAE) procedure of corn silk polysaccharides (CSPS) was established and the physicochemical properties, antioxidant and anticancer activities of CSPS were studied. Orthogonal test and response surface methodology were applied to optimize the extraction parameters. The optimum enzymolysis and ultrasonic conditions were cellulase content of 7.5% for 150 min at 55 °C and liquid-solid ratio of 31.8 for 34.2 min at 66.3 °C, respectively. Under these conditions, the yield of CSPS increased from 4.56% to 7.10%. CSPS obtained by hot water and EUAE were composed of rhamnose, arabinose, xylose, mannose, galactose and glucose with molecular ratios of 4.17:17.33:5.59:18.65:19.11:35.14 and 8.83:15.77:7.92:12.39:11.15:43.94, respectively. Their molecular weight distributions were 10.52 × 10(4) and 6.88 × 10(4)Da, respectively. CSPS obtained by EUAE showed morphological and conformation changes and higher antioxidant and anticancer activities compared with CSPS extracted by hot water.
authors.affiliation: Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China.
authors.forename: Shuhan
authors.initials: S
authors.lastname: Chen
authors.forename: Haixia
authors.initials: H
authors.lastname: Chen
authors.forename: Jingge
authors.initials: J
authors.lastname: Tian
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Wang
authors.forename: Lisha
authors.initials: L
authors.lastname: Xing
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D045744:Cell Line, Tumor; D002480:Cellulase / Q000378:metabolism*; D005591:Chemical Fractionation / Q000379:methods*; D055598:Chemical Phenomena; D006801:Humans; D006868:Hydrolysis; D017665:Hydroxyl Radical / Q000737:chemistry; D007501:Iron / Q000737:chemistry / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008970:Molecular Weight; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013696:Temperature; D013997:Time Factors; D014465:Ultrasonics; D003313:Zea mays / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D011134:Polysaccharides; D017665:Hydroxyl Radical; D007501:Iron; D002480:Cellulase; D001205:Ascorbic Acid
keywords: Antioxidant and anticancer activities; Chemical characteristics; Corn silk polysaccharides; Enzymolysis-ultrasonic assisted extraction