title: A Simple Protocol for the Generation of Cardiomyocytes from Human Pluripotent Stem Cells.
abstract: Efficient generation of cardiomyocytes from pluripotent stem cells (PSCs) for multiple downstream applications such as regenerative medicine, disease modeling, and drug screening remains a challenge. Cardiomyogenesis may be regulated in vitro by a controlled differentiation process, which involves various signaling molecules and extracellular environment. Here, we describe a simple method to efficiently generate cardiomyocytes from human embryonic stem cells and human induced pluripotent stem cells.
authors.affiliation: Research and Development Unit, National Heart Centre, Singapore, Singapore.
authors.forename: Glen Lester
authors.initials: GL
authors.lastname: Sequiera
authors.forename: Ashish
authors.initials: A
authors.lastname: Mehta
authors.forename: Winston
authors.initials: W
authors.lastname: Shim
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D058732:Embryoid Bodies / Q000166:cytology; D006801:Humans; D009200:Myocardial Contraction; D032383:Myocytes, Cardiac / Q000166:cytology*; D039904:Pluripotent Stem Cells / Q000166:cytology*