title: Neonatal olfactory bulbectomy enhances locomotor activity, exploratory behavior and binding of NMDA receptors in pre-pubertal rats.
abstract: In this study, we investigated the effect of neonatal olfactory bulbectomy (nOBX) on behavioral paradigms related to olfaction such as exploratory behavior, locomotor activity in a novel environment and social interaction. We also studied the effect of nOBX on the activity of the N-methyl-d-aspartate (NMDA) subtype of glutamate receptors during development. The behavioral effects of nOBX (postnatal day 7, PD7) were investigated in pre- (PD30) and post-pubertal (PD60) Wistar rats. NMDA receptor activity was measured with [(125)I]MK-801 in the brain regions associated with the olfactory circuitry. A significant increase in the novelty-induced locomotion was seen in the pre-pubertal nOBX rats. Although the locomotor effect was less marked than in pre-pubertal rats, the nOBX rats tested post-pubertally failed to habituate to the novel situation as quickly as the sham- and normal- controls. Pre-pubertally, the head-dipping behavior was enhanced in nOBX rats compared with sham-operated and normal controls, while normal exploratory behavior was observed between groups in adulthood. In contrast, social interaction was increased in post-pubertal animals that underwent nOBX. Both pre- and post-pubertal nOBX rats recovered olfaction. Interestingly, pre-pubertal rats showed a significant increase in the [(125)I]MK-801 binding in the piriform cortex, dorsal hippocampus, inner and outer layers of the frontal cortex and outer layer of the cingulate cortex. At post-pubertal age, no significant differences in [(125)I]MK-801 binding were observed between groups at any of the brain regions analyzed. These results suggest that nOBX produces pre-pubertal behavioral disturbances and NMDA receptor changes that are transitory with recovery of olfaction early in adulthood.
authors.affiliation: Laboratorio de Neuropsiquiatría, Instituto de Fisiología, Universidad Autónoma de Puebla, 14 Sur 6301, San Manuel, 72570 Puebla, Mexico; Douglas Mental Health University Institute, Department of Psychiatry, McGill University, Montréal, QC H4H 1R3, Canada. Electronic address: gonzaloflores56@gmail.com.
authors.forename: G
authors.initials: G
authors.lastname: Flores
authors.affiliation: Laboratorio de Neuropsiquiatría, Instituto de Fisiología, Universidad Autónoma de Puebla, 14 Sur 6301, San Manuel, 72570 Puebla, Mexico.
authors.forename: O
authors.initials: O
authors.lastname: Ibañez-Sandoval
authors.affiliation: Laboratorio de Neuropsiquiatría, Instituto de Fisiología, Universidad Autónoma de Puebla, 14 Sur 6301, San Manuel, 72570 Puebla, Mexico.
authors.forename: A B
authors.initials: AB
authors.lastname: Silva-Gómez
authors.affiliation: Laboratorio de Neuropsiquiatría, Instituto de Fisiología, Universidad Autónoma de Puebla, 14 Sur 6301, San Manuel, 72570 Puebla, Mexico.
authors.forename: I
authors.initials: I
authors.lastname: Camacho-Abrego
authors.affiliation: Laboratorio de Neurociencia Celular y Plasticidad, Universidad Pablo de Olavide, Sevilla, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Rodríguez-Moreno
authors.affiliation: Douglas Mental Health University Institute, Department of Psychiatry, McGill University, Montréal, QC H4H 1R3, Canada.
authors.forename: J C
authors.initials: JC
authors.lastname: Morales-Medina
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D016291:Dizocilpine Maleate / Q000493:pharmacokinetics; D005106:Exploratory Behavior / Q000502:physiology*; D005260:Female; D007398:Interpersonal Relations; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics; D008297:Male; D009043:Motor Activity / Q000502:physiology*; D009830:Olfactory Bulb / Q000254:growth & development / Q000502:physiology* / Q000601:surgery; D009833:Olfactory Pathways; D011247:Pregnancy; D011485:Protein Binding / Q000502:physiology; D011869:Radioligand Assay; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time / Q000502:physiology; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D012903:Smell / Q000502:physiology*; D018709:Statistics, Nonparametric; D013997:Time Factors
chemical_list: D007457:Iodine Radioisotopes; D016194:Receptors, N-Methyl-D-Aspartate; D016291:Dizocilpine Maleate
keywords: ANOVA; BL; Cg; DG; EDTA; HBT; MK-801; N-methyl-d-aspartate; NAcc; NMDA; NMDA receptor; OBX; OF; OT; PBS; PD; PFC; RMS; SI; SVZ; ablation of the olfactory bulbs; analysis of variance; basolateral; cingulate cortex; dPFC; dentate gyrus; dizocilpine; dorsal part of the prefrontal cortex; ethylenediaminetetraacetic acid; hole board test; limbic system; locomotion; nOBX; neonatal ablation of the olfactory bulbs; neonatal bilateral ablation of the olfactory bulbs; nucleus accumbens; olfactory bulbectomy and depression; olfactory tubercle; open field; phosphate saline solution; postnatal day; prefrontal cortex; rostral migratory stream; social interaction; subventricular zone