title: Improved glucose-neopentyl glycol (GNG) amphiphiles for membrane protein solubilization and stabilization.
abstract: Membrane proteins are inherently amphipathic and undergo dynamic conformational changes for proper function within native membranes. Maintaining the functional structures of these biomacromolecules in aqueous media is necessary for structural studies but difficult to achieve with currently available tools, thus necessitating the development of novel agents with favorable properties. This study introduces several new glucose-neopentyl glycol (GNG) amphiphiles and reveals some agents that display favorable behaviors for the solubilization and stabilization of a large, multi-subunit membrane protein assembly. Furthermore, a detergent structure-property relationship that could serve as a useful guideline for the design of novel amphiphiles is discussed.
authors.affiliation: Department of Bionanotechnology, Hanyang University, Ansan, 426-791 (Korea), Fax: (+81) 31-436-8146.
authors.forename: Kyung Ho
authors.initials: KH
authors.lastname: Cho
authors.forename: Hyoung Eun
authors.initials: HE
authors.lastname: Bae
authors.forename: Manabendra
authors.initials: M
authors.lastname: Das
authors.forename: Samuel H
authors.initials: SH
authors.lastname: Gellman
authors.forename: Pil Seok
authors.initials: PS
authors.lastname: Chae
mesh_terms: D003510:Cyclohexanes / Q000737:chemistry; D005947:Glucose / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D011409:Propylene Glycols / Q000737:chemistry*; D055550:Protein Stability; D016354:Rhodobacter capsulatus / Q000378:metabolism; D012995:Solubility; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D003510:Cyclohexanes; D045342:Light-Harvesting Protein Complexes; D008565:Membrane Proteins; D011409:Propylene Glycols; D013501:Surface-Active Agents; C506365:Cyclohexane; D005947:Glucose
keywords: amphiphiles; membrane proteins; molecular design; protein solubilization; protein stabilization