title: Cellulase-assisted extraction of polysaccharides from Cucurbita moschata and their antibacterial activity.
abstract: In this study, cellulase-assisted extraction of water soluble polysaccharides from pumpkin (Cucurbita moschata) and their antibacterial activity were investigated. The polysaccharides yield was monitored during the extraction process. The optimum extraction conditions were determined as follows: time, 40 min; temperature, 55°C; pH, 4.5; and cellulase amount, 4,000 U/g. The extracts were centrifuged, filtered, proteins removed by Sevag method, concentrated to approximately 15% (w/v), precipitated with 5 volumes of absolute ethanol, freeze-dried, and pulverized to yield a water soluble powder of pumpkin polysaccharides (PP). The sugar content of the product was 68.3%, and the yield was 17.34% (w/w), respectively. The PP had high antibacterial activity against Bacillus subtilis, Staphylococcus aureus, and Escherichia coli at the concentration of 100 mg/mL.
authors.affiliation: School of Food Engineering, Huaihai Institute of Technology, 59 Cangwu Road, Lianyungang 222005, China. Electronic address: qianzhiganghu@sina.cn.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Qian
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D002480:Cellulase / Q000378:metabolism*; D005591:Chemical Fractionation / Q000379:methods*; D028464:Cucurbita / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D013696:Temperature; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D011134:Polysaccharides; D002480:Cellulase
keywords: Antibacterial activities; Cellulase-assisted extraction; Cucurbita moschata; Polysaccharides