title: Evaluation of gelatinases and IL-6 in the cerebrospinal fluid of patients with optic neuritis, multiple sclerosis and other inflammatory neurological diseases.
abstract: The activities of the metalloproteinase gelatinase B, and the presence of IL-6, an inducer of metalloproteinase inhibitors, were investigated in CSF samples of 190 patients with multiple sclerosis (MS; n = 55), optic neuritis (ON; n = 46), other inflammatory neurological diseases (OIND; n = 27) or control patients (CON) with non-inflammatory neurological diseases (n = 62). IL-6, measurable as hybridoma growth factor activity (detection limit 3 pg/ml), was found in only four of these 190 CSF samples (three OIND, one CON). Elevated CSF gelatinase B levels were detected in 40%, 35% and 54% of the patients with MS, ON and OIND, respectively, while all control CSFs were devoid of gelatinase B activity. Clinical and laboratory data were compared with gelatinase B levels. No correlation was found between the CSF cytoses and gelatinase B levels, suggesting that this enzyme in the CSF originates from CNS lesions rather than from CSF cells. However, the occurrence of the gelatinase B significantly correlated with the IgG index in the MS patient group. This study stimulates further investigation into the possible usage of protease inhibition in demyelinating diseases.
authors.affiliation: Rega Institute for Medical Research, University of Leuven, Minderbroedersstraat 10, 3000 Leuven, BelgiumDepartment of Neurology, Huddinge University Hospital, Karolinska Institute, Stockholm, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Paemen
authors.forename: T
authors.initials: T
authors.lastname: Olsson
authors.forename: M
authors.initials: M
authors.lastname: Söderström
authors.forename: J
authors.initials: J
authors.lastname: van Damme
authors.forename: G
authors.initials: G
authors.lastname: Opdenakker