title: Efficient translation of long-lived messengers in extracts from dry pea primary axes : Evidence for the presence of lectin mRNA.
abstract: Extracts prepared from dry pea (Pisum sativum, L; cv oberon) primary axes translate efficiently their endogenous messengers in an in vitro protein synthesizing system. The native long-lived messengers are biologically fully active and direct the synthesis of a whole range of polypeptides with MW ranging up to 130,000. About 0.5% of the total in vitro synthesized polypeptides are recovered in the immunoprecipitate obtained with pea lectin antiserum. Since about one-fourth of the radioactivity in the immunoprecipitate comigrates with authentic pea lectin it is concluded that about 0.1% of the long-lived messengers code for the lectin.
authors.affiliation: Laboratorium voor Plantenbiochemie, Katholieke Universiteit Leuven, Vaartstraat 24, B-3000, Leuven, Belgium.
authors.forename: W J
authors.initials: WJ
authors.lastname: Peumans
authors.forename: B M
authors.initials: BM
authors.lastname: Delaey
authors.forename: A
authors.initials: A
authors.lastname: Manickam
authors.forename: A R
authors.initials: AR
authors.lastname: Carlier