title: Interaction with bovine serum albumin of an anti-oxidative pectic arabinogalactan from Andrographis paniculata.
abstract: A pectic arabinogalactan was obtained from the leaves of Andrographis paniculata by aqueous extraction followed by α-amylase treatment, deproteination, and anion exchange chromatography. Methylation analysis, Smith degradation, and NMR spectroscopy indicated that it was a highly branched arabinogalactan containing a (1→3)-linked β-d-Galp main chain, substituted at O-6 by (1→6)-linked β-d-Galp side chains. The latter residues were substituted at O-3 by (1→5)- and (1→3)-linked α-l-Araf chains, and non reducing end-units of α-l-Araf and β-d-Galp. This homogeneous arabinogalactan (36 kDa), which contained phenolic acids, showed dose-dependent anti-oxidative properties. The phenolic acid moieties might be the functional sites. This arabinogalactan can form a complex with bovine serum albumin having binding constant K=6.48 × 10(6)/M. Thus, this study is an important step forward to investigate the involvement of arabinogalactan in processes including interaction with biologically important transport proteins.
authors.affiliation: Natural Products Laboratory, Department of Chemistry, The University of Burdwan, West Bengal 713104, India.
authors.forename: Udipta R
authors.initials: UR
authors.lastname: Chatterjee
authors.forename: Sayani
authors.initials: S
authors.lastname: Ray
authors.forename: Valérie
authors.initials: V
authors.lastname: Micard
authors.forename: Debjani
authors.initials: D
authors.lastname: Ghosh
authors.forename: Kanika
authors.initials: K
authors.lastname: Ghosh
authors.forename: Shruti S
authors.initials: SS
authors.lastname: Bandyopadhyay
authors.forename: Bimalendu
authors.initials: B
authors.lastname: Ray
mesh_terms: D035685:Andrographis / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D005685:Galactans / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006031:Glycosylation; D007501:Iron / Q000737:chemistry; D010084:Oxidation-Reduction; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D055672:Static Electricity
chemical_list: D016166:Free Radical Scavengers; D005685:Galactans; D012710:Serum Albumin, Bovine; D007501:Iron; C005653:arabinogalactan
keywords: Andrographis paniculata; Anti-oxidative effect; Pectic arabinogalactan; Protein–polysaccharide complex