title: Precipitation of hydroxyapatite on electrospun polycaprolactone/aloe vera/silk fibroin nanofibrous scaffolds for bone tissue engineering.
abstract: Advances in electrospun nanofibres with bioactive materials have enhanced the scope of fabricating biomimetic scaffolds for tissue engineering. The present research focuses on fabrication of polycaprolactone/aloe vera/silk fibroin nanofibrous scaffolds by electrospinning followed by hydroxyapatite deposition by calcium-phosphate dipping method for bone tissue engineering. Morphology, composition, hydrophilicity and mechanical properties of polycaprolactone/aloe vera/silk fibroin-hydroxyapatite nanofibrous scaffolds along with controls polycaprolactone and polycaprolactone/aloe vera/silk fibroin nanofibrous scaffolds were examined by field emission scanning electron microscopy, Fourier transform infrared spectroscopy, contact angle and tensile tests, respectively. Adipose-derived stem cells cultured on polycaprolactone/aloe vera/silk fibroin-hydroxyapatite nanofibrous scaffolds displayed highest cell proliferation, increased osteogenic markers expression (alkaline phosphatase and osteocalcin), osteogenic differentiation and increased mineralization in comparison with polycaprolactone control. The obtained results indicate that polycaprolactone/aloe vera/silk fibroin-hydroxyapatite nanofibrous scaffolds have appropriate physico-chemical and biological properties to be used as biomimetic scaffolds for bone tissue regeneration.
authors.affiliation: Department of Textile Technology, Anna University, Chennai, India Centre for Biotechnology, Anna University, Chennai, India.
authors.forename: Suganya
authors.initials: S
authors.lastname: Shanmugavel
authors.affiliation: Nanoscience and Nanotechnology Initiative, National University of Singapore, Singapore.
authors.forename: Venugopal Jayarama
authors.initials: VJ
authors.lastname: Reddy
authors.affiliation: Nanoscience and Nanotechnology Initiative, National University of Singapore, Singapore.
authors.forename: Seeram
authors.initials: S
authors.lastname: Ramakrishna
authors.affiliation: Centre for Biotechnology, Anna University, Chennai, India.
authors.forename: B S
authors.initials: BS
authors.lastname: Lakshmi
authors.affiliation: Department of Textile Technology, Anna University, Chennai, India vrgiridev@yahoo.com.
authors.forename: Vr Giri
authors.initials: VG
authors.lastname: Dev
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000469:Alkaline Phosphatase / Q000378:metabolism; D000504:Aloe / Q000737:chemistry; D040761:Biomimetic Materials / Q000737:chemistry; D018786:Bone Substitutes / Q000737:chemistry*; D049109:Cell Proliferation; D002478:Cells, Cultured; D011232:Chemical Precipitation; D017886:Durapatite / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis; D011091:Polyesters / Q000737:chemistry; D013234:Stem Cells / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D018786:Bone Substitutes; D011091:Polyesters; D015675:Osteocalcin; C016240:polycaprolactone; D005349:Fibroins; D017886:Durapatite; D000469:Alkaline Phosphatase
keywords: Aloe vera; bone tissue engineering; electrospinning; hydroxyapatite; silk fibroin