title: Degradation of LIM domain-binding protein three during processing of Spanish dry-cured ham.
abstract: Extensive proteolysis takes place during the processing of dry-cured ham due to the action of muscle peptidases. The aim of this work was to study the degradation of LIM domain binding protein 3 (LDB3), which is located at the Z-lines of the sarcomere, at different times during the Spanish dry-cured ham processing (2, 3.5, 5, 6.5, and 9 months). A total of 107 peptides have been identified by mass spectrometry, most of them generated from the first region of the protein sequence (position 1-90) providing evidence for the complexity and variability of proteolytic reactions throughout the whole process of dry-curing. Methionine oxidation has been observed in several peptides by the end of the process. The potential of some of the identified peptides to be used as biomarkers of dry-cured ham processing has also been considered.
authors.affiliation: Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Avenue Agustín Escardino 7, 46980 Paterna, Valencia, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Gallego
authors.forename: Leticia
authors.initials: L
authors.lastname: Mora
authors.forename: Paul D
authors.initials: PD
authors.lastname: Fraser
authors.forename: María-Concepción
authors.initials: MC
authors.lastname: Aristoy
authors.forename: Fidel
authors.initials: F
authors.lastname: Toldrá
mesh_terms: D000818:Animals; D005511:Food Handling / Q000379:methods*; D060850:LIM-Homeodomain Proteins / Q000737:chemistry* / Q000378:metabolism; D008461:Meat Products / Q000032:analysis*; D009132:Muscles / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D059748:Proteolysis; D013030:Spain; D013552:Swine
chemical_list: D060850:LIM-Homeodomain Proteins
keywords: BLAST; BSA; Biomarker; Dry-cured ham; ETD; FA; HCl; HPLC; LDB3; LIM domain binding protein 3; LIM domain-binding protein 3; MS; Mass spectrometry; NCBInr; National center for biotechnology information non-redundant; Peptide oxidation; Peptides; Proteolysis; RSLC; SDS–PAGE; Uniprot; Z-band alternatively spliced PDZ-motif protein; ZASP; basic local alignment search tool; bovine serum albumin; electron transfer dissociation; formic acid; high performance liquid chromatography; hydrochloric acid; mass spectrometry; nLC; nanoliquid chromatography; rapid separation liquid chromatography; sodium dodecyl sulphate polyacrylamide gel electrophoresis; universal protein resource