title: Characterizing the natural canthaxanthin/2-hydroxypropyl-β-cyclodextrin inclusion complex.
abstract: A fundamental study on the phase-solubility, storage stability and antioxidant activity of the inclusion complex of 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) with canthaxanthin (CTX) biosynthesized by Dietzia natronolimnaea HS-1 in a continuous bioreactor was designed. A variety of in vitro chemical methods including reducing power (RP), nitrite-scavenging activity (NSA), and 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and hydroxyl radicals scavenging capacities was used to determine antioxidant activities of the CTX and CTX/HP-β-CD complex. Results revealed that the apparent stability constant of the inclusion complex was 669 M(-1) in a 1:1 stoichiometric ratio. The complexation of CTX with HP-β-CD significantly improved its water solubility and storage stability. An increased dose-dependent manner was found for the NSA and RP values of the CTX and CTX/HP-β-CD complex. The CTX/HP-β-CD complex showed higher scavenging capacity than native CTX against DPPH, ABTS and hydroxyl radicals. It can be used as a promising strategy to improve the food application of CTX.
authors.affiliation: Bioprocess Engineering Laboratory (BPEL), Department of Food Science, Engineering and Technology, Faculty of Agricultural Engineering and Technology, University of Tehran, P.O. Box 4111, Karaj 31587-77871, Iran.
authors.forename: Seyed Mohammad Taghi
authors.initials: SM
authors.lastname: Gharibzahedi
authors.forename: Seyed Hadi
authors.initials: SH
authors.lastname: Razavi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Mousavi
mesh_terms: D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000192:Actinomycetales / Q000378:metabolism; D016644:Canthaxanthin / Q000737:chemistry*; D004355:Drug Stability; D005285:Fermentation; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000378:metabolism; D005609:Free Radicals / Q000737:chemistry; D010084:Oxidation-Reduction; D012995:Solubility; D047392:beta-Cyclodextrins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D016166:Free Radical Scavengers; D005609:Free Radicals; D047392:beta-Cyclodextrins; D000073738:2-Hydroxypropyl-beta-cyclodextrin; D016644:Canthaxanthin
keywords: Carotenoid pigment; Chemical stability; Dietzia natronolimnaea HS-1; Hydroxypropyl-β-cyclodextrin; Inclusion complex characterization; Solubility enhancement