title: Micropatterned polymeric nanosheets for local delivery of an engineered epithelial monolayer.
abstract: Like a carpet for cells, micropatterned polymeric nanosheets are developed toward local cell delivery. The nanosheets direct morphogenesis of retinal pigment epithelial (RPE) cells and allow for the injection of an engineered RPE monolayer through syringe needles without the loss of cell viability. Such an ultrathin carrier has the promise of a minimally invasive delivery of cells into narrow tissue spaces.
authors.affiliation: WPI-Advanced Institute for Materials Research (WPI-AIMR), Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, 980-8577, Japan; Department of Life Science and Medical Bioscience, School of Advanced Science and Engineering, Waseda University, TWIns, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo, 162-8480, Japan.
authors.forename: Toshinori
authors.initials: T
authors.lastname: Fujie
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Mori
authors.forename: Shuntaro
authors.initials: S
authors.lastname: Ito
authors.forename: Matsuhiko
authors.initials: M
authors.lastname: Nishizawa
authors.forename: Hojae
authors.initials: H
authors.lastname: Bae
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Nagai
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Onami
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Abe
authors.forename: Ali
authors.initials: A
authors.lastname: Khademhosseini
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kaji
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival; D066298:In Vitro Techniques; D008266:Macula Lutea / Q000502:physiology; D008268:Macular Degeneration; D049329:Nanostructures / Q000737:chemistry / Q000627:therapeutic use*; D013508:Ophthalmologic Surgical Procedures; D018583:Pliability; D011108:Polymers / Q000737:chemistry; D051381:Rats; D020318:Rats, Long-Evans; D055213:Retinal Pigment Epithelium / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D013314:Stress, Mechanical; D013552:Swine; D013594:Syringes; D019108:Tight Junctions / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D062826:Zonula Occludens-1 Protein / Q000378:metabolism
chemical_list: D011108:Polymers; C569044:Tjp1 protein, rat; D062826:Zonula Occludens-1 Protein
keywords: cell delivery; magnetic nanoparticles (MNP); retinal pigment epithelium (RPE); tight junctions; ultrathin films (nanosheets)