title: A simple and highly sensitive ELISA for screening of the α-thalassemia-1 Southeast Asian-type deletion.
abstract: Couples in which both partners carry the α-thalassemia-1 trait have a 25% risk of hemoglobin Bart's hydrops fetalis in each pregnancy. Identification of α-thalassemia-1 trait is, therefore, necessary in order to control this severe form of α-thalassemia. We have generated monoclonal antibodies specific to the ζ-globin chain without cross reaction with other globin chains. A simple and sensitive ELISA was developed by using poly-l-lysine to increase the protein binding to the ELISA plate. The developed poly-l-lysine pre-coated ELISA has a very high sensitivity (100%) and specificity (97%) for detection of carriers of α-thalassemia-1 with Southeast Asian-type deletion.
authors.affiliation: a Biomedical Technology Research Center, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency at the Faculty of Associated Medical Sciences, Chiang Mai University , Chiang Mai , Thailand.
authors.forename: Supansa
authors.initials: S
authors.lastname: Pata
authors.forename: Saichit
authors.initials: S
authors.lastname: Khummuang
authors.forename: Sakorn
authors.initials: S
authors.lastname: Pornprasert
authors.forename: Thanusak
authors.initials: T
authors.lastname: Tatu
authors.forename: Watchara
authors.initials: W
authors.lastname: Kasinrerk
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D001210:Asia, Southeastern; D044466:Asian People / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D017353:Gene Deletion; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity; D017085:alpha-Thalassemia / Q000175:diagnosis* / Q000235:genetics*; D055543:zeta-Globins / Q000032:analysis* / Q000235:genetics* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D055543:zeta-Globins