title: Use of Multicolor Flow Cytometry for Isolation of Specific Cell Populations Deriving from Differentiated Human Embryonic Stem Cells.
abstract: Flow Cytometry-Sorting (FCM-Sorting) is a technique commonly used to identify and isolate specific types of cells from a heterogeneous population of live cells. Here we describe a multicolor flow cytometry technique that uses five distinct cell surface antigens to isolate four live populations with different surface antigen profiles. These profiles were used to help distinguishing between neural and nonneural (the lens) ectoderm derivatives within a highly heterogenous population of differentiating human embryonic stem cells (hESC).
authors.affiliation: Department of Experimental Cardiology, Academic Medical Center, University of Amsterdam, K2-113, Meibergdreef 9, 1105AZ, Amsterdam, The Netherlands, i.mengarelli@amc.uva.nl.
authors.forename: Isabella
authors.initials: I
authors.lastname: Mengarelli
authors.forename: Andrew
authors.initials: A
authors.lastname: Fryga
authors.forename: Tiziano
authors.initials: T
authors.lastname: Barberi
mesh_terms: D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism; D000066449:Human Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans
chemical_list: D005456:Fluorescent Dyes