title: Short chain N-acylhomoserine lactone production by clinical multidrug resistant Klebsiella pneumoniae strain CSG20.
abstract: Klebsiella pneumoniae is one of the most common Gram-negative bacterial pathogens in clinical practice. It is associated with a wide range of disorders, ranging from superficial skin and soft tissue infections to potentially fatal sepsis in the lungs and blood stream. Quorum sensing, or bacterial cell-cell communication, refers to population density-dependent gene expression modulation. Quorum sensing in Proteobacteria relies on the production and sensing of signaling molecules which are mostly N-acylhomoserine lactones. Here, we report the identification of a multidrug resistant clinical isolate, K. pneumoniae strain CSG20, using matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry. We further confirmed quorum sensing activity in this strain with the use of high resolution tandem liquid chromatography quadrupole mass spectrometry and provided evidence K. pneumoniae strain CSG20 produced N-hexanoyl-homoserine lactone (C6-HSL). To the best of our knowledge, this is the first report on the production of N-hexanoylhomoserine lactone (C6-HSL) in clinical isolate K. pneumoniae.
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia. kokgan@um.edu.my.
authors.forename: Yun Fong
authors.initials: YF
authors.lastname: Ngeow
authors.forename: Huey Jia
authors.initials: HJ
authors.lastname: Cheng
authors.forename: Jian Woon
authors.initials: JW
authors.lastname: Chen
authors.forename: Wai-Fong
authors.initials: WF
authors.lastname: Yin
authors.forename: Kok-Gan
authors.initials: KG
authors.lastname: Chan
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000378:metabolism*; D053038:Quorum Sensing
chemical_list: D054742:Acyl-Butyrolactones; D000900:Anti-Bacterial Agents