title: Naringin accelerates the regression of pre-neoplastic lesions and the colorectal structural reorganization in a murine model of chemical carcinogenesis.
abstract: The aim of this study was to investigate the effect of Naringin on pre-neoplastic colorectal lesions induced by chemical carcinogen in rats. Female Wistar rats weighing 130.8±27.1 g received weekly one subcutaneous injection of 1,2-dimethylhydrazine (DMH, 20 mg/kg) for 10 weeks. The animals were divided into 5 groups with 6 animals in each group. Group 1: 0.9% saline; Group 2: DMH+0.9% saline; Group 3: DMH+Naringin (10 mg/kg); Group 4: DMH+Naringin (100 mg/kg); Group 5: DMH+Naringin (200 mg/kg). G2 and G3 showed a significant increase in ACF number, AgNOR/nucleus and mitosis compared to G1. G4 and G5 presented a significant reduction in these parameters compared to G2. The number of cells producing acidic and neutral mucins, red blood cells and the level of antioxidant minerals, such as copper, magnesium, selenium and zinc, were significantly reduced in G2 and G3, but similar in G4 and G5 compared to G1. Naringin, especially at 200 mg/kg, was effective in reducing the number of pre-neoplastic lesions in rats exposed to DMH. Some of these effects may be due to reduction in cellular proliferation and tissue levels of iron together with the recovery of antioxidant mineral levels induced by this flavonoid.
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Viçosa, 36570-000 MG, Brazil.
authors.forename: Priscila L
authors.initials: PL
authors.lastname: Sequetto
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Viçosa, 36570-000 MG, Brazil.
authors.forename: Tânia T
authors.initials: TT
authors.lastname: Oliveira
authors.affiliation: Department of General Biology, Federal University of Viçosa, 36570-000 MG, Brazil.
authors.forename: Izabel R S C
authors.initials: IR
authors.lastname: Maldonado
authors.affiliation: Department of Veterinary Medicine, Federal University of Viçosa, 36570-000 MG, Brazil.
authors.forename: Luís Eugênio F
authors.initials: LE
authors.lastname: Augusto
authors.affiliation: Institute of Biological Sciences, Federal University of Para, 66075-110 PA, Brazil.
authors.forename: Vanessa J
authors.initials: VJ
authors.lastname: Mello
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Viçosa, 36570-000 MG, Brazil.
authors.forename: Virginia R
authors.initials: VR
authors.lastname: Pizziolo
authors.affiliation: Applied Biotechnology Institute (BIOAGRO), Federal University of Viçosa, 36570-000 MG, Brazil.
authors.forename: Márcia R
authors.initials: MR
authors.lastname: Almeida
authors.affiliation: Department of Biological Sciences and NUPEB, Federal University of Ouro Preto, 35400-000 MG, Brazil.
authors.forename: Marcelo E
authors.initials: ME
authors.lastname: Silva
authors.affiliation: Department of Biological Sciences and NUPEB, Federal University of Ouro Preto, 35400-000 MG, Brazil; Department of Cell, Tissue and Developmental Biology, Federal University of Alfenas, 37130-000 MG, Brazil. Electronic address: romuonovaes@yahoo.com.br.
authors.forename: Rômulo D
authors.initials: RD
authors.lastname: Novaes
mesh_terms: D019813:1,2-Dimethylhydrazine / Q000633:toxicity; D000818:Animals; D002273:Carcinogens / Q000633:toxicity; D015179:Colorectal Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D005260:Female; D044950:Flavanones / Q000494:pharmacology*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011230:Precancerous Conditions / Q000139:chemically induced / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002273:Carcinogens; D044950:Flavanones; D019813:1,2-Dimethylhydrazine; C005274:naringin
keywords: 1,2-Dimethylhydrazine; 1,2-dimethylhydrazine; 8-OHdG; 8-hydroxy-2′-deoxyguanosine; AB; ACF; AgNORs; Alcian blue; DMH; EDS; Flavonoide; Large intestine; PAS; Pathology; Toxicology; Vv; X-ray energy dispersive spectroscopy; aberrant crypt foci; argyrophilic nucleolar organizing regions; periodic acid-schiff; volume density