title: Intratumoral gene therapy versus intravenous gene therapy for distant metastasis control with 2-diethylaminoethyl-dextran methyl methacrylate copolymer non-viral vector-p53.
abstract: Lung cancer still remains to be challenged by novel treatment modalities. Novel locally targeted routes of administration are a methodology to enhance treatment and reduce side effects. Intratumoral gene therapy is a method for local treatment and could be used either in early-stage lung cancer before surgery or at advanced stages as palliative care. Novel non-viral vectors are also in demand for efficient gene transfection to target local cancer tissue and at the same time protect the normal tissue. In the current study, C57BL/6 mice were divided into three groups: (a) control, (b) intravenous and (c) intatumoral gene therapy. The novel 2-Diethylaminoethyl-Dextran Methyl Methacrylate Copolymer Non-Viral Vector (Ryujyu Science Corporation) was conjugated with plasmid pSicop53 from the company Addgene for the first time. The aim of the study was to evaluate the safety and efficacy of targeted gene therapy in a Lewis lung cancer model. Indeed, although the pharmacokinetics of the different administration modalities differs, the intratumoral administration presented increased survival and decreased distant metastasis. Intratumoral gene therapy could be considered as an efficient local therapy for lung cancer.
authors.affiliation: Department of Pathology, "G. Papanikolaou" General Hospital, Thessaloniki, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Baliaka
authors.affiliation: 1] Department of Pulmonary, "G. Papanikolaou" General Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece [2] Department of Interventional Pneumology, Ruhrlandklinik, West German Lung Center, University Hospital, University Duisburg-Essen, Essen, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Zarogoulidis
authors.affiliation: Department of Pulmonary, "G. Papanikolaou" General Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: K
authors.initials: K
authors.lastname: Domvri
authors.affiliation: Department of II Medical, "Coburg" Regional Department, University of Wuerzburg, Coburg, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Hohenforst-Schmidt
authors.affiliation: Department of Pathology, "G. Papanikolaou" General Hospital, Thessaloniki, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Sakkas
authors.affiliation: Department of Respiratory Diseases, Changhai Hospital/First Affiliated Hospital of the Second Military Medical University, Shanghai, China.
authors.forename: H
authors.initials: H
authors.lastname: Huang
authors.affiliation: Interventional Drug Delivery Systems and Strategies (ID2S2), Medical Cryogenics, Lakeland Court Jupiter, Jupiter, FL, USA.
authors.forename: P
authors.initials: P
authors.lastname: Le Pivert
authors.affiliation: Department of Biochemistry, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: G
authors.initials: G
authors.lastname: Koliakos
authors.affiliation: Department of Biochemistry, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Koliakou
authors.affiliation: Department of Histology-Embryology, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: K
authors.initials: K
authors.lastname: Kouzi-Koliakos
authors.affiliation: Department of Cardiothoracic Surgery, "Saint Luke" Private Hospital of Health Excellence, Thessaloniki, Greece.
authors.forename: K
authors.initials: K
authors.lastname: Tsakiridis
authors.affiliation: Experimental Animal Laboratory, "G. Papanikolaou" General Hospital, Thessaloniki, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Chioti
authors.affiliation: Experimental Animal Laboratory, "G. Papanikolaou" General Hospital, Thessaloniki, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Siotou
authors.affiliation: Department of Pathology, "G. Papanikolaou" General Hospital, Thessaloniki, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Cheva
authors.affiliation: Department of Pulmonary, "G. Papanikolaou" General Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: K
authors.initials: K
authors.lastname: Zarogoulidis
authors.affiliation: Department of Pathology, "G. Papanikolaou" General Hospital, Thessaloniki, Greece.
authors.forename: L
authors.initials: L
authors.lastname: Sakkas
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000009:adverse effects*; D018827:Carcinoma, Lewis Lung / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D003637:DEAE-Dextran / Q000008:administration & dosage / Q000009:adverse effects*; D015316:Genetic Therapy / Q000009:adverse effects / Q000379:methods; D020366:Methylmethacrylate / Q000008:administration & dosage / Q000009:adverse effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis / Q000628:therapy*; D010957:Plasmids / Q000008:administration & dosage; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D016159:Tumor Suppressor Protein p53; D020366:Methylmethacrylate; D003637:DEAE-Dextran