title: Protein expression profiling by antibody array analysis with use of dried blood spot samples on filter paper.
abstract: Dried blood spot samples (DBSS) on filter paper offer several advantages compared to conventional serum/plasma samples: they do not require any phlebotomy or separation of blood by centrifugation; they are less invasive; they allow sample stability and shipment at room temperature; and they pose a negligible risk of infection with blood-borne viruses, such as HIV, HBV and HCV, to those who handle them. Therefore dried blood spot samples (DBSS) on filter paper can be a quick, convenient and inexpensive means of obtaining blood samples for biomarker discovery, disease screening, diagnosis and treatment monitoring in non-hospitalized, public health settings. In this study, we investigated for the first time the potential application of dried blood spot samples (DBSS) in protein expression profiling using antibody array technology. First, optimal conditions for array assay performance using dried blood spot samples (DBSS) was established, including sample elution buffer, elution time, elution temperature and assay blocking buffer. Second, we analyzed dried blood spot samples (DBSS) using three distinct antibody array platforms, including sandwich-based antibody arrays, quantitative antibody arrays and biotin-label-based antibody arrays. In comparison with paired serum samples, detection of circulating proteins in dried blood spot samples (DBSS) correlated well for both low- and high-abundance proteins on all three antibody array platforms. In conclusion, our study strongly indicates the novel application of multiplex antibody array platforms to analyze dried blood spot samples (DBSS) on filter paper represents a viable, cost-effective method for protein profiling, biomarker discovery and disease screening in a large, population-based survey.
authors.affiliation: RayBiotech Inc., 3607 Parkway Lane, Norcross, GA 30092, USA.
authors.forename: Weidong
authors.initials: W
authors.lastname: Jiang
authors.affiliation: RayBiotech Inc., 3607 Parkway Lane, Norcross, GA 30092, USA; RayBiotech Inc., Guangzhou 510600, China.
authors.forename: Ying Qing
authors.initials: YQ
authors.lastname: Mao
authors.affiliation: RayBiotech Inc., 3607 Parkway Lane, Norcross, GA 30092, USA; RayBiotech Inc., Guangzhou 510600, China.
authors.forename: Ruochun
authors.initials: R
authors.lastname: Huang
authors.affiliation: Department of Clinical Laboratory, The Second Affiliated Hospital of Sun Yet-sen University, Guangzhou 510120, China.
authors.forename: Chaohui
authors.initials: C
authors.lastname: Duan
authors.affiliation: Department of Clinical Laboratory, The Third Affiliated Hospital of Sun Yet-sen University, Guangzhou 51063, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xi
authors.affiliation: Department of Dermatology, People's Hospital of Liaoning Province, Shenyang 110005, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.affiliation: RayBiotech Inc., 3607 Parkway Lane, Norcross, GA 30092, USA; RayBiotech Inc., Guangzhou 510600, China; South China Biochip Research Center, Guangzhou 51063 China. Electronic address: rhuang@raybiotech.com.
authors.forename: Ruo-Pan
authors.initials: RP
authors.lastname: Huang
mesh_terms: D000906:Antibodies; D015415:Biomarkers / Q000097:blood; D019921:Biotinylation; D002021:Buffers; D016207:Cytokines / Q000097:blood* / Q000276:immunology; D059788:Dried Blood Spot Testing; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods*; D015203:Reproducibility of Results; D013696:Temperature
chemical_list: D000906:Antibodies; D015415:Biomarkers; D002021:Buffers; D016207:Cytokines
keywords: Antibody array; Biomarkers; Dried blood spot samples; Proteomics; Serum