title: Association study of the estrogen receptor I gene (ESR1) in anorexia nervosa and eating disorders: no replication found.
abstract: OBJECTIVE
  The female preponderance and onset around puberty in the majority of eating disorders (EDs) suggest that sex hormones, like estrogens, may be involved in the onset of these disorders. An eight-SNP haplotype at the estrogen receptor I (ESR1) gene was found to be associated with anorexia nervosa (AN) (Versini et al., Neuropsychopharmacology, 35, 1818-1825, 2010) and three SNPs from this haplotype (rs726281, rs2295193, and rs3798577) were associated with AN and/or EDs. Our objective was to replicate these findings in an independent cohort of 520 patients with an eating disorder, of whom 244 had AN (142 restricting type) from the GenED study and 2,810 random women from the Netherlands Twin Registry.
  
  
  METHODS
  The frequencies of the eight-SNP haplotype and three ESR1 SNPs were compared between patients with an eating disorder, with AN (restricting type), with bulimia nervosa (BN), and the control women.
  
  
  RESULTS
  Neither the haplotype nor the three ESR1 SNPs were associated with EDs, BN, AN, or restricting type AN.
  
  
  CONCLUSIONS
  Despite sufficient statistical power, the associations reported by Versini et al. (Neuropsychopharmacology, 35, 1818-1825, 2010) were not replicated.
authors.affiliation: Center for Eating Disorders Ursula, Leidschendam, The Netherlands; Leiden University Medical Centre, Molecular Epidemiology Section (Department of Medical Statistics), Leiden, The Netherlands; Department of Biological Psychology, VU University, Amsterdam, The Netherlands.
authors.forename: Margarita C T
authors.initials: MC
authors.lastname: Slof-Op 't Landt
authors.forename: Eric F
authors.initials: EF
authors.lastname: van Furth
authors.forename: Ingrid
authors.initials: I
authors.lastname: Meulenbelt
authors.forename: Meike
authors.initials: M
authors.lastname: Bartels
authors.forename: Jouke Jan
authors.initials: JJ
authors.lastname: Hottenga
authors.forename: P Eline
authors.initials: PE
authors.lastname: Slagboom
authors.forename: Dorret I
authors.initials: DI
authors.lastname: Boomsma
mesh_terms: D000856:Anorexia Nervosa / Q000235:genetics*; D052018:Bulimia Nervosa / Q000235:genetics; D047628:Estrogen Receptor alpha / Q000235:genetics*; D001068:Feeding and Eating Disorders / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha
keywords: ESR1; anorexia nervosa; eating disorders; estrogen; genetic association