title: Adipose-derived stem cells exhibit antioxidative and antiapoptotic properties to rescue ischemic acute kidney injury in rats.
abstract: BACKGROUND
  Acute kidney injury is a major challenge in critical care medicine, with high rates of in-hospital morbidity and mortality. Stem cell therapy has emerged as an evolving technology that could have a substantial impact on acute kidney injury outcomes in the critical care environment. Therefore, the authors investigated the therapeutic effects of adipose-derived stem cells in ischemic acute kidney injury in rats.
  
  
  METHODS
  The authors used an ischemia-reperfusion-induced acute kidney injury rat model. The effects of rescuing acute kidney injury were assessed with regard to different adipose-derived stem cell numbers and various routes of administration compared with sham-operated and phosphate-buffered saline-treated groups.
  
  
  RESULTS
  Both intrarenal arterial and intravenous administration of adipose-derived stem cells reduced blood urea nitrogen and creatinine levels, and also decreased the tubular injury score 48 hours after ischemia-reperfusion-induced acute kidney injury in a dose-dependent manner, compared with the phosphate-buffered saline-treated group. In the authors' study, it was determined that the optimal cell number was 5 × 10. Furthermore, adipose-derived stem cell transplantation exhibited antioxidative and antiinflammatory properties to reduce apoptosis and promote proliferation of renal tubular cells.
  
  
  CONCLUSIONS
  An optimal number of adipose-derived stem cells administered by means of the intrarenal arterial or the intravenous route effectively rescued ischemia-reperfusion-induced acute kidney injury in rats. Antioxidative and antiapoptotic properties of adipose-derived stem cells to reduce tubular cell injury also merit recognition and further study.
authors.affiliation: Taipei, Taiwan From the Division of Plastic Surgery, Department of Surgery, Division of Nephrology, Department of Medicine, and Department of Neurosurgery, Neurological Institute, Taipei Veterans General Hospital; and School of Medicine, Department and Institute of Physiology, Institute of Clinical Medicine, and Institute of Pharmacology, National Yang-Ming University.
authors.forename: Yu-Chung
authors.initials: YC
authors.lastname: Shih
authors.forename: Pei-Ying
authors.initials: PY
authors.lastname: Lee
authors.forename: Henrich
authors.initials: H
authors.lastname: Cheng
authors.forename: Chi-Han
authors.initials: CH
authors.lastname: Tsai
authors.forename: Hsu
authors.initials: H
authors.lastname: Ma
authors.forename: Der-Cherng
authors.initials: DC
authors.lastname: Tarng
mesh_terms: D058186:Acute Kidney Injury / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D007684:Kidney Tubules / Q000378:metabolism / Q000473:pathology; D008297:Male; D018384:Oxidative Stress / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D016896:Treatment Outcome