title: Use of peptide nucleic acids (PNAs) for genotyping by solution and surface methods.
abstract: Peptide nucleic acids (PNAs) are synthetic oligonucleotide analogues based on a pseudopeptide backbone that bind complementary DNA or RNA with high affinity and specificity. In this chapter, three PNA-based genotyping assays are described: PCR clamping, fluorescence-based recognition, and microarray platform. The first two methods are performed in solution, while the microarray method uses a solid surface.
authors.affiliation: Department of Food Science, University of Parma, Parma, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Sforza
authors.forename: Tullia
authors.initials: T
authors.lastname: Tedeschi
authors.forename: Mariangela
authors.initials: M
authors.lastname: Bencivenni
authors.forename: Alessandro
authors.initials: A
authors.lastname: Tonelli
authors.forename: Roberto
authors.initials: R
authors.lastname: Corradini
authors.forename: Rosangela
authors.initials: R
authors.lastname: Marchelli
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D060005:Genotyping Techniques / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011804:Quinolines / Q000737:chemistry; D012996:Solutions; D013499:Surface Properties; D014158:Transcription, Genetic
chemical_list: D052160:Benzothiazoles; D015345:Oligonucleotide Probes; D020135:Peptide Nucleic Acids; D011804:Quinolines; D012996:Solutions; C051000:thiazole orange