title: Fumonisin measurement from maize samples by high-performance liquid chromatography coupled with corona charged aerosol detector.
abstract: Fumonisins are a class of mycotoxins produced mainly by Fusarium species, which is primary fungal contaminant of the maize and maize-derived products around the world. The B-series fumonisins (FB1, FB2 and FB3) are the most abundant and toxic constituent; thus, their levels are regulated generally worldwide. In this study, we developed a reliable method for the measurement of fumonisin FB1, FB2 and FB3 mycotoxins from maize samples without the time-consuming derivatization step using a high-performance liquid chromatograph coupled with corona charged aerosol detector. The detection and quantitation limit of the whole method were 0.02 and 0.04 mg/kg for each fumonisins, respectively. The detection linearity was tested in the calibration range of 2 orders of magnitude and the recoveries from the spiked samples were determined. The developed method proved to be sufficient to measure the maximum residue levels of fumonisins, which are specified in European Union and United States in maize and maize-based products.
authors.affiliation: Faculty of Science and Informatics, Department of Microbiology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary andras.j.szekeres@gmail.com.
authors.forename: András
authors.initials: A
authors.lastname: Szekeres
authors.affiliation: Edison House Holding Co., Ltd., Hernádi dűlő 18., H-2724 Újlengyel, Hungary.
authors.forename: Andrea
authors.initials: A
authors.lastname: Budai
authors.affiliation: Faculty of Science and Informatics, Department of Microbiology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary.
authors.forename: Ottó
authors.initials: O
authors.lastname: Bencsik
authors.affiliation: Edison House Holding Co., Ltd., Hernádi dűlő 18., H-2724 Újlengyel, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Németh
authors.affiliation: Fumizol Ltd., Moszkvai krt. 5-7, H-6725 Szeged, Hungary.
authors.forename: Tibor
authors.initials: T
authors.lastname: Bartók
authors.affiliation: Research Centre for Agriculture, Hungarian Academy of Sciences, Research Institute for Plant Protection, Herman Ottó út 15, H-1022 Budapest, Hungary.
authors.forename: Arpád
authors.initials: A
authors.lastname: Szécsi
authors.affiliation: Cereal Research Non-profit Ltd., Alsókikötő sor 9, H-6726 Szeged, Hungary.
authors.forename: Akos
authors.initials: A
authors.lastname: Mesterházy
authors.affiliation: Faculty of Science and Informatics, Department of Microbiology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary.
authors.forename: Csaba
authors.initials: C
authors.lastname: Vágvölgyi
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D037341:Fumonisins / Q000032:analysis* / Q000737:chemistry; D005670:Fusarium / Q000737:chemistry*; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D003313:Zea mays / Q000382:microbiology*
chemical_list: D037341:Fumonisins