title: A Validated High-performance Liquid Chromatograhy Method for Estimation of Ferulic Acid in Asafoetida and Polyherbal Preparation.
abstract: A high-performance liquid chromatography method was developed for the estimation of ferulic acid from asafoetida and a polyherbal preparation. The separation was carried out on HiQSil ODS C-18 column with a mobile phase of acetonitrile: 10% acetic acid (20:80 v/v). The developed method was validated as per International Conference of Harmonization guidelines for various parameters such as accuracy, precision, linearity, limit of detection, limit of quantification and specificity; and found to be reliable. Linear regression analysis showed a good corelation between peak area and concentration with a corelation coefficient r(2)=0.996 in the range 200-7000 ng/ml. The developed method can be utilized for standardization of herbal formulation comprising asafoetida.
authors.affiliation: Department of Quality Assurance, Bharati Vidyapeeth's College of Pharmacy, Belapur, Navi Mumbai-400 614, India.
authors.forename: J A
authors.initials: JA
authors.lastname: Kareparamban
authors.forename: P H
authors.initials: PH
authors.lastname: Nikam
authors.forename: A P
authors.initials: AP
authors.lastname: Jadhav
authors.forename: V J
authors.initials: VJ
authors.lastname: Kadam
keywords: High-performance liquid chromatograhy; asafoetida; ferulic acid; polyherbal formulations.