title: Quantitative ion localization within Suaeda maritima leaf mesophyll cells.
abstract: Grown under saline conditions, Suaeda maritima accumulates Na(+) and Cl(-) into its leaves, where individual mesophyll cells behave differently in their compartmentation of these ions. Measurements of ion concentrations within selected subcellular compartments show that freeze-substitution with dry sectioning is a valuable preparative technique for analytical electron microscopy of highly vacuolate plant material. Using this approach, absolute estimates were made of Na(+), K(+) and Cl(-) concentrations in the cytoplasm, cell walls, chloroplasts and vacuoles of leaf mesophyll cells.
authors.affiliation: School of Biological Sciences, University of Sussex, BN1 9QG, Brighton, Sussex, UK.
authors.forename: D M
authors.initials: DM
authors.lastname: Harvey
authors.forename: J L
authors.initials: JL
authors.lastname: Hall
authors.forename: T J
authors.initials: TJ
authors.lastname: Flowers
authors.forename: B
authors.initials: B
authors.lastname: Kent