title: Comprehensive multi-channel multi-dimensional counter-current chromatography for separation of tanshinones from Salvia miltiorrhiza Bunge.
abstract: Multi-dimensional chromatography offers the increased resolution and peak capacity by coupling of multiple columns with the same or different separation mechanisms. In this work, a novel multi-channel multi-dimensional counter-current chromatography (CCC) has been successfully constructed and used for several two-dimensional (2D) and three-dimensional (3D) CCC separations including 2D A×B/A×C, A×B-C and A-B×C, and 3D A×B×C systems. These 2D and 3D CCC systems were further applied to separate the bioactive tanshinones from the extract of Tanshen (or Danshen, Salvia miltiorrhiza Bunge), a famous Traditional Chinese Medicine (TCM). As a result, the developed 2D and 3D CCC methods were successful and efficient for resolving the tanshinones from complex extracts. Compared to the 1D multiple columns CCC separation, the 2D and 3D CCC decrease analysis time, reduce solvent consumption and increase sample throughput significantly. It may be widely used for current drug development, metabolomic analysis and natural product isolation.
authors.affiliation: Research Center of Siyuan Natural Pharmacy and Biotoxicology, College of Life Sciences Zhejiang University, Hangzhou 310058, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Meng
authors.affiliation: Research Center of Siyuan Natural Pharmacy and Biotoxicology, College of Life Sciences Zhejiang University, Hangzhou 310058, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Research Center of Siyuan Natural Pharmacy and Biotoxicology, College of Life Sciences Zhejiang University, Hangzhou 310058, China.
authors.forename: Junling
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang Province 310058, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Research Center of Siyuan Natural Pharmacy and Biotoxicology, College of Life Sciences Zhejiang University, Hangzhou 310058, China. Electronic address: drwushihua@zju.edu.cn.
authors.forename: Shihua
authors.initials: S
authors.lastname: Wu
mesh_terms: D045784:Abietanes / Q000302:isolation & purification*; D001688:Biological Products / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D055432:Metabolomics / Q000379:methods; D010618:Phenanthrolines / Q000737:chemistry; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013997:Time Factors
chemical_list: D045784:Abietanes; D001688:Biological Products; D004365:Drugs, Chinese Herbal; D010618:Phenanthrolines; D012997:Solvents; C021751:tanshinone; C028972:dan-shen root extract
keywords: Counter-current chromatography; Multi-dimensional liquid chromatography; Salvia miltiorrhiza Bunge; Tanshinones; Three-dimensional chromatography; Two-dimensional chromatography