title: Structural insights into how 5-hydroxymethylation influences transcription factor binding.
abstract: Transcription factor binding and high resolution crystallographic studies (1.3 Å) of Dickerson-Drew duplexes with cytosine, methylcytosine and hydroxymethylcytosine bases provide evidence that C-5 cytosine modifications could regulate transcription by context dependent effects on DNA transcription factor interactions.
authors.affiliation: Department of Chemistry and the Oxford Centre for Integrative Systems Biology, Chemistry Research Laboratory, Oxford, UK. Christopher.schofield@chem.ox.ac.uk.
authors.forename: Lukas
authors.initials: L
authors.lastname: Lercher
authors.forename: Michael A
authors.initials: MA
authors.lastname: McDonough
authors.forename: Afaf H
authors.initials: AH
authors.lastname: El-Sagheer
authors.forename: Armin
authors.initials: A
authors.lastname: Thalhammer
authors.forename: Skirmantas
authors.initials: S
authors.lastname: Kriaucionis
authors.forename: Tom
authors.initials: T
authors.lastname: Brown
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Schofield
mesh_terms: D044503:5-Methylcytosine / Q000031:analogs & derivatives; D003596:Cytosine / Q000031:analogs & derivatives* / Q000378:metabolism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D019175:DNA Methylation; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010084:Oxidation-Reduction; D011485:Protein Binding; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D014157:Transcription Factors; C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA