title: Calcium phosphate transfection of primary hippocampal neurons.
abstract: Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neurons cocultured with astroglial cells.
authors.affiliation: Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, Rutgers University.
authors.forename: Miao
authors.initials: M
authors.lastname: Sun
authors.forename: Laura P
authors.initials: LP
authors.lastname: Bernard
authors.forename: Victoria L
authors.initials: VL
authors.lastname: Dibona
authors.forename: Qian
authors.initials: Q
authors.lastname: Wu
authors.forename: Huaye
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000502:physiology; D002130:Calcium Phosphates / Q000008:administration & dosage* / Q000737:chemistry; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D051381:Rats; D014162:Transfection / Q000379:methods*
chemical_list: D002130:Calcium Phosphates; D017077:Culture Media, Conditioned; D004247:DNA; C020243:calcium phosphate