title: A recombinant influenza virus vaccine expressing the F protein of respiratory syncytial virus.
abstract: Infections with influenza and respiratory syncytial virus (RSV) rank high among the most common human respiratory diseases worldwide. Previously, we developed a replication-incompetent influenza virus by replacing the coding sequence of the PB2 gene, which encodes one of the viral RNA polymerase subunits, with that of a reporter gene. Here, we generated a PB2-knockout recombinant influenza virus expressing the F protein of RSV (PB2-RSVF virus) and tested its potential as a bivalent vaccine. In mice intranasally immunized with the PB2-RSVF virus, we detected high levels of antibodies against influenza virus, but not RSV. PB2-RSVF virus-immunized mice were protected from a lethal challenge with influenza virus but experienced severe body weight loss when challenged with RSV, indicating that PB2-RSVF vaccination enhanced RSV-associated disease. These results highlight one of the difficulties of developing an effective bivalent vaccine against influenza virus and RSV infections.
authors.affiliation: Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, Mexico, Mexico.
authors.forename: Wendy
authors.initials: W
authors.lastname: Fonseca
authors.forename: Makoto
authors.initials: M
authors.lastname: Ozawa
authors.forename: Masato
authors.initials: M
authors.lastname: Hatta
authors.forename: Esther
authors.initials: E
authors.lastname: Orozco
authors.forename: Máximo B
authors.initials: MB
authors.lastname: Martínez
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kawaoka
mesh_terms: D000818:Animals; D005260:Female; D015967:Gene Expression Regulation, Viral; D057809:HEK293 Cells; D006801:Humans; D007252:Influenza Vaccines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000517:prevention & control*; D018357:Respiratory Syncytial Virus Infections / Q000517:prevention & control; D012136:Respiratory Syncytial Viruses / Q000276:immunology* / Q000378:metabolism; D014614:Vaccines, Synthetic / Q000276:immunology; D014760:Viral Fusion Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D007252:Influenza Vaccines; D014614:Vaccines, Synthetic; D014760:Viral Fusion Proteins