title: Determination of residues of three triphenylmethane dyes and their metabolites (malachite green, leuco malachite green, crystal violet, leuco crystal violet, and brilliant green) in aquaculture products by LC/MS/MS: first action 2012.25.
abstract: During the AOAC Annual Meeting held from September 30 to October 3, 2012 in Las Vegas, NV, the Expert Review Panel (ERP) on Veterinary Drug Residues reviewed data for the method for determination of residues of three triphenylmethane dyes and their metabolites (malachite green, leuco malachite green, crystal violet, leuco crystal violet, and brilliant green) in aquaculture products by LC/MS/MS, previously published in the Journal of Chromatography A 1218, 1632-1645 (2006). The method data were reviewed and compared to the standard method performance requirements (SMPRs) found in SMPR 2009.001, published in AOAC's Official Methods of Analysis, 19th Ed. (2012). The ERP determined that the data were acceptable, and the method was approved AOAC Official First Action. The method uses acetonitrile to isolate the analyte from the matrix. Then determination is conducted by LCIMS/MS with positive electrospray ionization. Accuracy ranged from 100.1 to 109.8% for samples fortified at levels of 0.5, 0.75, 1.0, and 2.0 microg/kg. Precision ranged from 2.0 to 10.3% RSD for the intraday samples and 1.9 to 10.6% for the interday samples analyzed over 3 days. The described method is designed to accurately operate in the analytical range from 0.5 to 2 microg/kg, where the minimum required performance limit for laboratories has been fixed in the European Union at 2.0 microg/kg for these banned substances and their metabolites. Upper levels of concentrations (1-100 microg/kg) can be analyzed depending on the different optional calibrations used.
authors.affiliation: ANSES, French Agency for Food, Environmental and Occupational Health Safety, Fougères Laboratory, Veterinary Drug Residues Unit, EU-RL for Antibiotic and Dye Residues, La Haute Marche, Javen6, BP 90203, 35302 Fougères, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Hurtaud-Pessel
authors.forename: Pierrick
authors.initials: P
authors.lastname: Couëdor
authors.forename: Eric
authors.initials: E
authors.lastname: Verdon
authors.forename: Dawn
authors.initials: D
authors.lastname: Dowell
mesh_terms: D017756:Aquaculture; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004396:Coloring Agents / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D005840:Gentian Violet / Q000032:analysis; D000644:Quaternary Ammonium Compounds / Q000032:analysis; D012394:Rosaniline Dyes / Q000032:analysis; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014320:Triphenylmethyl Compounds / Q000032:analysis*
chemical_list: D004396:Coloring Agents; D000644:Quaternary Ammonium Compounds; D012394:Rosaniline Dyes; D014320:Triphenylmethyl Compounds; C005095:malachite green; C046945:triphenylmethane; C015320:leucomalachite green; C006798:brilliant green; D005840:Gentian Violet