title: IL-17E production is elevated in the lungs of Balb/c mice in the later stages of Chlamydia muridarum infection and re-infection.
abstract: BACKGROUND
  Pathogens can influence allergic respiratory diseases. We previously found that multiple infections with Chlamydophila pneumoniae induce the production of interleukin-17A (IL-17A) and IL-17E, which have roles in the pathogenesis of asthma. The present work was designed to investigate our hypothesis that infections with another pathogen can induce the production of IL-17A and IL-17E.
  
  
  METHODS
  At an internal of 28 days, mice were infected twice with Chlamydia muridarum; the kinetics of IL-17A and IL-17E expression was subsequently determined at the mRNA and protein levels. The amounts of IL-17 cytokines produced by the stimulated spleen cells were determined by enzyme-linked immunosorbent assay (ELISA). The presence of IL-17E in the lungs was revealed by an indirect immunofluorescence test.
  
  
  RESULTS
  The infection with C. muridarum induced the production of IL-17A at the early stages of infection. The quantity of IL-17E was highest on days 28 and 56 after the first infection (28 days after the second infection). In the later stages of infection, IL-17E was produced by epithelial cells. The re-stimulated peripheral spleen cells produced IL-17A.
  
  
  CONCLUSIONS
  Multiple infection with C. muridarum induces the production of a high amount of IL-17E, which plays an important part in the pathogenesis of allergic pulmonary diseases.
authors.affiliation: MD Department of Medical Microbiology and Immunobiology, University of Szeged, H-6720 Szeged, Dóm tér 10., Hungary. burian.katalin@med.u-szeged.hu.
authors.forename: Tímea
authors.initials: T
authors.lastname: Mosolygó
authors.forename: Gabriella
authors.initials: G
authors.lastname: Spengler
authors.forename: Valéria
authors.initials: V
authors.lastname: Endrész
authors.forename: Krisztián
authors.initials: K
authors.lastname: Laczi
authors.forename: Katalin
authors.initials: K
authors.lastname: Perei
authors.forename: Katalin
authors.initials: K
authors.lastname: Burián
mesh_terms: D056809:Alveolar Epithelial Cells / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D000818:Animals; D001980:Bronchi / Q000378:metabolism; D002690:Chlamydia Infections / Q000276:immunology / Q000378:metabolism*; D023502:Chlamydia muridarum / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism*; D008168:Lung / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D018410:Pneumonia, Bacterial / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D012008:Recurrence
chemical_list: D020381:Interleukin-17
keywords: Animal models; Chlamydia muridarum; IL-17A; IL-17E; allergic pulmonary diseases