title: Dexamethasone-loaded hydroxyapatite enhances bone regeneration in rat calvarial defects.
abstract: A combination of bioceramics and osteogenic factors is potentially useful for bone regeneration applications. In the present study, hydroxyapatite particles (HA) were loaded with dexamethasone (Dex) and then characterized using SEM and drug release study. The bone regeneration ability of Dex-loaded HA (Dex/HA) was investigated in a rat critical size bone defect using digital mammography, multislice spiral-computed tomography (MSCT) imaging, and histological analysis. The HA and Dex/HA showed nano and micro-scale morphology with a nearly homogenous distribution of diameter. In addition, about 90 % of the drug was released from Dex/HA over a period of three days. After 8 weeks of implantation in rat calvarial defects, no sign of inflammation or complication was observed at the site of surgery. According to digital mammography and MSCT, Dex/HA showed the highest bone regeneration in rat bone defects compared to those received drug-free HA. Histological studies confirmed these data and showed osteointegration to the surrounding tissue. Taking all together, it was demonstrated that Dex/HA can be used as an appropriate synthetic graft for bone tissue engineering applications. These newly developed bioceramics can be used as new bone graft substitutes in orthopaedic surgery and is capable of enhancing bone regeneration.
authors.affiliation: Department of Orthopedic Surgery, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran, rezatavakolidarestani@yahoo.com.
authors.forename: Reza
authors.initials: R
authors.lastname: Tavakoli-darestani
authors.forename: Alireza
authors.initials: A
authors.lastname: Manafi-rasi
authors.forename: Amin
authors.initials: A
authors.lastname: Kamrani-rad
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D003907:Dexamethasone / Q000737:chemistry / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D005938:Glucocorticoids / Q000737:chemistry / Q000494:pharmacology*; D006882:Hydroxyapatites / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D010316:Particle Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000187:drug effects / Q000502:physiology*
chemical_list: D004337:Drug Carriers; D005938:Glucocorticoids; D006882:Hydroxyapatites; D003907:Dexamethasone