title: Detection of host-specific immunogenic proteins in the saliva of patients with oral squamous cell carcinoma.
abstract: The main purpose of this article is to develop a new and reliable saliva-based clinical diagnostic method for the early detection of oral squamous cell carcinoma (OSCC). This study used an immunoproteomic approach which allowed the detection of immunogenic host proteins in patients' samples using pooled human antibodies. In an attempt to investigate potential biomarkers of OSCC, two-dimensional electrophoresis (2-DE) followed by immunoblotting of saliva from patients and controls were compared. The protein spots of interest were analyzed using 2-DE image analyzer and subsequently subjected to MALDI-TOF/TOF and then matched against NCBI database. The result showed that four protein clusters, namely Human Pancreatic Alpha-amylase (HPA), Human Salivary Amylase (sAA), keratin-10 (K-10), and Ga Module Complexed with Human Serum Albumin (GA-HSA), had exhibited immunoreactivity in western blot. The results are suggestive of the potential use of the differentially expressed saliva protein as tumor biomarkers for the detection of OSCC. However, further studies are recommended to validate this finding.
authors.affiliation: a Dental Research and Training Unit, Faculty of Dentistry, University of Malaya , Kuala Lumpur , Malaysia.
authors.forename: Alan Kang-Wai
authors.initials: AK
authors.lastname: Mu
authors.forename: Yunn Shy
authors.initials: YS
authors.lastname: Chan
authors.forename: Szu Szu
authors.initials: SS
authors.lastname: Kang
authors.forename: Siti Nuraishah
authors.initials: SN
authors.lastname: Azman
authors.forename: Rosnah Binti
authors.initials: RB
authors.lastname: Zain
authors.forename: Wen Lin
authors.initials: WL
authors.lastname: Chai
authors.forename: Yeng
authors.initials: Y
authors.lastname: Chen
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000276:immunology*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D053550:Keratin-10 / Q000032:analysis / Q000276:immunology; D009062:Mouth Neoplasms / Q000175:diagnosis* / Q000276:immunology; D055292:Pancreatic alpha-Amylases / Q000032:analysis / Q000276:immunology; D012463:Saliva / Q000737:chemistry* / Q000201:enzymology / Q000276:immunology*; D055291:Salivary alpha-Amylases / Q000032:analysis / Q000276:immunology
chemical_list: D014408:Biomarkers, Tumor; C507493:KRT10 protein, human; D053550:Keratin-10; D055292:Pancreatic alpha-Amylases; D055291:Salivary alpha-Amylases