title: Production of heparan sulfate in the process of calcium oxalate microlith formation.
abstract: To evaluate the interaction in the renal tubules between calcium oxalate microliths and glycosaminoglycans, especially heparan sulfate, we planned an experimentally-induced calcium oxalate microlith-producing model in rats. We found by immunofluorescence study and immunoelectron microscopy using the heparan sulfate antibody that heparan sulfate was produced in the renal tubular cells in the formation of calcium oxalate microliths and that heparan sulfate exists in the microliths. Heparan sulfate/crystal interaction might play a role as a nucleating agent and promote stone enlargement.
authors.affiliation: Department of Urology, Ehime University School of Medicine, Ehime, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Nishio
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Yokoyama
authors.forename: H
authors.initials: H
authors.lastname: Iwata
authors.forename: M
authors.initials: M
authors.lastname: Takeuchi
mesh_terms: D000818:Animals; D002129:Calcium Oxalate / Q000737:chemistry / Q000378:metabolism*; D003460:Crystallization; D004195:Disease Models, Animal; D005455:Fluorescent Antibody Technique; D006497:Heparan Sulfate / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D007684:Kidney Tubules / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D046529:Microscopy, Electron, Transmission; D016253:Microscopy, Immunoelectron; D051381:Rats; D017208:Rats, Wistar; D052878:Urolithiasis / Q000378:metabolism*
chemical_list: D002129:Calcium Oxalate; D006497:Heparan Sulfate
keywords: calcium oxalate microlith; heparan sulfate; immunoelectron microscopy; renal tubules