title: Time-profiling fluorescent reporters in the Arabidopsis root.
abstract: Confocal laser scanning microscopy is a useful nondestructive approach for the visualization of fluorescent reporters in planta. Samples are usually placed between a slide and a cover slip which, although suited to single time-point imaging, does not allow long-term observation. Here, we describe a technique to monitor changes in fluorescence in the Arabidopsis root over a long period of time. Treatment can easily be performed, and this approach is suitable for use in low-throughput chemical screens. We also present a rapid method to analyze fluorescence intensity profiles generated using this protocol.
authors.affiliation: Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Sutton Bonington, UK.
authors.forename: Antoine P
authors.initials: AP
authors.lastname: Larrieu
authors.forename: Andrew P
authors.initials: AP
authors.lastname: French
authors.forename: Tony P
authors.initials: TP
authors.lastname: Pridmore
authors.forename: Malcolm J
authors.initials: MJ
authors.lastname: Bennett
authors.forename: Darren M
authors.initials: DM
authors.lastname: Wells
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism*; D046508:Culture Techniques; D004353:Drug Evaluation, Preclinical / Q000379:methods; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism*; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism*
chemical_list: D010937:Plant Growth Regulators