title: Rubi Fructus (Rubus coreanus) Inhibits Differentiation to Adipocytes in 3T3-L1 Cells.
abstract: Rubi Fructus (RF) is known to exert several pharmacological effects including antitumor, antioxidant, and anti-inflammatory activities. However, its antiobesity effect has not been reported yet. This study was focused on the antidifferentiation effect of RF extract on 3T3-L1 preadipocytes. When 3T3-L1 preadipocytes were differentiating into adipocytes, 10-100  μ g/mL of RF was added. Next, the lipid contents were quantified by Oil Red O staining. RF significantly reduced lipid accumulation and downregulated the expression of peroxisome proliferator-activated receptor γ (PPAR γ ), CCAAT0-enhancer-binding proteins α (C/EBP α ), adipocyte fatty acid-binding protein 2 (aP2), resistin, and adiponectin in ways that were concentration dependent. Moreover, RF markedly upregulated liver kinase B1 and AMP-activated protein kinase (AMPK). Interestingly, pretreatment with AMPK α siRNA and RF downregulated the expression of PPAR γ and C/EBP α protein as well as the adipocyte differentiation. Our study shows that RF is capable of inhibiting the differentiation of 3T3-L1 adipocytes through the modulation of PPAR γ , C/EBP α , and AMPK, suggesting that it has a potential for therapeutic application in the treatment or prevention of obesity.
authors.affiliation: Department of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Jeonbuk 570-749, Republic of Korea.
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Jeong
authors.forename: Hye-Lin
authors.initials: HL
authors.lastname: Kim
authors.forename: Jinbong
authors.initials: J
authors.lastname: Park
authors.forename: Hyo-Jin
authors.initials: HJ
authors.lastname: An
authors.forename: Sung-Hoon
authors.initials: SH
authors.lastname: Kim
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Hong-Seob
authors.initials: HS
authors.lastname: So
authors.forename: Raekil
authors.initials: R
authors.lastname: Park
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Um
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Hong