title: Pulsed counter-current ultrasound-assisted extraction and characterization of polysaccharides from Boletus edulis.
abstract: Four methods for extracting polysaccharides from Boletus edulis, namely, hot-water extraction, ultrasonic clearer extraction, static probe ultrasonic extraction, and pulsed counter-current probe ultrasonic extraction (CCPUE), were studied. Results showed that CCPUE has the highest extraction efficiency among the methods studied. Under optimal CCPUE conditions, a B. edulis polysaccharide (BEP) yield of 8.21% was obtained. Three purified fractions, BEP-I, BEP-II, and BEP-III, were obtained through sequential purification by DEAE-52 and Sephadex G-75 chromatography. The average molecular weights of BEP-I, BEP-II, and BEP-III were 10,278, 23,761, and 42,736 Da, respectively. The polysaccharides were mainly composed of xylose, mannose, galactose, and glucose; of these, mannose contents were the highest. The antioxidant activities of the BEPs were further investigated by measurement of their ability to scavenge DPPH and hydroxyl radicals as well as their reducing power. The results indicated that the BEPs have good antioxidant activity.
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223001, China; Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaian 223300, China; Huaian High-Tech Research Institute of Nanjing University, Huaian 223001, China.
authors.forename: Qinghong
authors.initials: Q
authors.lastname: You
authors.forename: Xiulian
authors.initials: X
authors.lastname: Yin
authors.forename: Chaowen
authors.initials: C
authors.lastname: Ji
mesh_terms: D001487:Basidiomycota / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods*; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification*; D005656:Fungal Proteins / Q000032:analysis; D017665:Hydroxyl Radical / Q000737:chemistry; D009005:Monosaccharides / Q000032:analysis; D010084:Oxidation-Reduction; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D013696:Temperature; D013997:Time Factors; D014465:Ultrasonics; D014574:Uronic Acids / Q000032:analysis
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005656:Fungal Proteins; D009005:Monosaccharides; D010851:Picrates; D011134:Polysaccharides; D014574:Uronic Acids; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant activity; Boletus edulis polysaccharide; Pulsed counter-current ultrasonic extraction; Purification