title: [Genetic diagnosis against fungal cerebromeningitis].
abstract: Fungal cerebromeningitis is one of deep seated mycoses and also a fatal fungal infectious disease. Regarding to causative pathogen of fungal cerebromeningitis in Japan, Cryptococcus spp., Candida spp., Aspergillus spp., are popular fungi. In general, the diagnosis of deep seated mycosis is sometime difficult. The genetic diagnosis method such as PCR against deep seated mycosis has been developing and it has been also reported as one of useful diagnostic tests. However, PCR for fungal detection is still a research test that has not been cleared or approved officially, therefore it should not be used for diagnosis, or patient management routinely. The PCR which detect broad range of fungi or specific fungus is applied for clinical situation, a careful attention should be paid for avoiding contamination because many fungal species are available in living environment.
authors.affiliation: Department of Chemotherapy and Mycoses, National Institute of Infectious diseases.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ohno
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Miyazaki
mesh_terms: D001230:Aspergillus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D002175:Candida / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D020314:Central Nervous System Fungal Infections / Q000175:diagnosis* / Q000382:microbiology*; D003454:Cryptococcus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D004196:Disease Outbreaks; D006801:Humans; D008590:Meningoencephalitis / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D014481:United States / Q000453:epidemiology