title: Characterization of the spinach chloroplast genes for the S4 ribosomal protein, tRNA(Thr) (UGU) and tRNA (Ser) (GGA).
abstract: The map location and nucleotide sequence of the genes for the S4 ribosomal protein (rps4) and for tRNA(Thr) (UGU) (trnT) and tRNA(Ser) (GGA) (trnS) on spinach chloroplast DNA have been determined. rps4 lies approximately 5 kb 3' to atpBE in the large single copy region and is transcribed in the same direction as atpBE. It has a 178 bp leader sequence, a 603 bp coding region and 620 bp 3' tail. The sequence of the coding region is 83% homologous with that of maize rps4 (29) and the deduced amino acid sequences from the two species are 7% homologous. The spinach and Escherichia coli S4 proteins are only 36% homologous. As in the case of maize, trnT lies upstream from and on the same strand as rps4 whereas trnS lies downstream and on the opposite strand. Transcription of rps4 apparently proceeds past trnS.
authors.affiliation: Division of Plant Industry, CSIRO, GPO Box 1600, 2601, Canberra, A.C.T., Australia.
authors.forename: S B
authors.initials: SB
authors.lastname: Tahar
authors.forename: W
authors.initials: W
authors.lastname: Bottomley
authors.forename: P R
authors.initials: PR
authors.lastname: Whitfeld