title: Role of stem cells in large animal genetic engineering in the TALENs-CRISPR era.
abstract: The establishment of embryonic stem cells (ESCs) and gene targeting technologies in mice has revolutionised the field of genetics. The relative ease with which genes can be knocked out, and exogenous sequences introduced, has allowed the mouse to become the prime model for deciphering the genetic code. Not surprisingly, the lack of authentic ESCs has hampered the livestock genetics field and has forced animal scientists into adapting alternative technologies for genetic engineering. The recent discovery of the creation of induced pluripotent stem cells (iPSCs) by upregulation of a handful of reprogramming genes has offered renewed enthusiasm to animal geneticists. However, much like ESCs, establishing authentic iPSCs from the domestic animals is still beset with problems, including (but not limited to) the persistent expression of reprogramming genes and the lack of proven potential for differentiation into target cell types both in vitro and in vivo. Site-specific nucleases comprised of zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regulated interspaced short palindromic repeats (CRISPRs) emerged as powerful genetic tools for precisely editing the genome, usurping the need for ESC-based genetic modifications even in the mouse. In this article, in the aftermath of these powerful genome editing technologies, the role of pluripotent stem cells in livestock genetics is discussed.
authors.affiliation: Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USA.
authors.forename: Ki-Eun
authors.initials: KE
authors.lastname: Park
authors.forename: Bhanu Prakash V L
authors.initials: BP
authors.lastname: Telugu
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002460:Cell Line; D065150:Cellular Reprogramming; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D003851:Deoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018390:Gene Targeting / Q000662:veterinary; D018014:Gene Transfer Techniques / Q000662:veterinary; D005818:Genetic Engineering / Q000662:veterinary*; D005838:Genotype; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism*; D058751:Livestock / Q000235:genetics*; D010641:Phenotype; D012260:Ribonucleases / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D014157:Transcription Factors; D003851:Deoxyribonucleases; D012260:Ribonucleases