title: BioPEGylation of polyhydroxybutyrate promotes nerve cell health and migration.
abstract: This study reports on the superior suitability of Polyhydroxybutyrate-polyethylene glycol hybrid polymers biosynthesised by Cupriavidus necator over PHB as biomaterials for tissue engineering. Incorporation of PEG106 (DEG) during PHB biosynthesis reduced crystallinity, molecular weight, and hydrophobicity while improving mechanical properties. In vitro olfactory ensheathing cell (OEC) proliferation was enhanced by cultivation on PHB-b-DEG films. Cultivation on PHB and PHB-b-DEG films showed no cytotoxic responses and cell viability and membrane integrity was sustained. PHB-b-DEG films promoted OECs entering into the DNA replication (S) phase and mitotic (G2-M) phase during the cell growth cycle and apoptosis was low. This study also confirmed an association between the level of neurite-outgrowth inhibitory protein (Nogo) and receptor pair Ig-like receptor B (PirB) expression and cell proliferation, both being down-regulated in cells grown on hybrid films when compared with PHB and asynchronous growth. Thus, DEG-terminated PHB-based biomaterials have great potential as biological scaffolds supporting nerve repair.
authors.affiliation: Bio/Polymer Research Group, Centre for Advanced Macromolecular Design, School of Biotechnology and Biomolecular Science, and §Infection and Inflammation Research Centre, School of Medical Sciences, University of New South Wales , Sydney, New South Wales, Australia.
authors.forename: Rodman T H
authors.initials: RT
authors.lastname: Chan
authors.forename: Robert A
authors.initials: RA
authors.lastname: Russell
authors.forename: Helder
authors.initials: H
authors.lastname: Marçal
authors.forename: Terry H
authors.initials: TH
authors.lastname: Lee
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Holden
authors.forename: L John R
authors.initials: LJ
authors.lastname: Foster
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D020118:Cupriavidus necator; D006885:Hydroxybutyrates / Q000737:chemistry* / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D014961:X-Ray Diffraction
chemical_list: D006885:Hydroxybutyrates; D011108:Polymers; D011092:Polyethylene Glycols