title: Bone marrow-derived macrophage production.
abstract: Macrophages are critical components of the innate and adaptive immune responses, and they are the first line of defense against foreign invaders because of their powerful microbicidal activities. Macrophages are widely distributed throughout the body and are present in the lymphoid organs, liver, lungs, gastrointestinal tract, central nervous system, bone, and skin. Because of their repartition, they participate in a wide range of physiological and pathological processes. Macrophages are highly versatile cells that are able to recognize microenvironmental alterations and to maintain tissue homeostasis. Numerous pathogens have evolved mechanisms to use macrophages as Trojan horses to survive, replicate in, and infect both humans and animals and to propagate throughout the body. The recent explosion of interest in evolutionary, genetic, and biochemical aspects of host-pathogen interactions has renewed scientific attention regarding macrophages. Here, we describe a procedure to isolate and cultivate macrophages from murine bone marrow that will provide large numbers of macrophages for studying host-pathogen interactions as well as other processes.
authors.affiliation: CNRS UMR 7278, IRD198, INSERM U1095, Aix-Marseille Université
authors.forename: Virginie
authors.initials: V
authors.lastname: Trouplin
authors.forename: Nicolas
authors.initials: N
authors.lastname: Boucherit
authors.forename: Laurent
authors.initials: L
authors.lastname: Gorvel
authors.forename: Filippo
authors.initials: F
authors.lastname: Conti
authors.forename: Giovanna
authors.initials: G
authors.lastname: Mottola
authors.forename: Eric
authors.initials: E
authors.lastname: Ghigo
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D003584:Cytological Techniques / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects; D051379:Mice
chemical_list: D016178:Granulocyte-Macrophage Colony-Stimulating Factor