title: The association of IL-8-251T/A polymorphism with complicated malaria in Karbi Anglong district of Assam.
abstract: Amongst host genetic factors, cytokine gene polymorphism can be anticipated to be an important factor as qualitative, quantitative and time of secretion play an important role in disease outcome. We have investigated association of cytokine promoter SNPs with risk of Plasmodium falciparum malaria and disease severity in a case control study in malaria endemic Karbi Anglong district of Assam, India. Frequency of IL-8-251T/A (p=0.03 and p=0.01) and TGF-β1-509C/T (p=0.02 and p=0.03) was higher in malaria in comparison to control participants and non-malarial fever controls. Interestingly, a higher frequency of mutant allele of IL-10-819T/C was observed in non-malarial fever controls compared to malaria thus suggesting its role as a distinguishing marker of the two disease groups. Higher IL-8 expression and increased frequency of IL-8-251T/A in complicated malaria (p=0.002) was reported indicating its role in susceptibility to complicated malaria. In conclusion, our study suggests the role of mutant genotype of IL-8-251T/A as a marker of complicated malaria in our population. Surprisingly, decreased expression of TGF-β1 in uncomplicated malaria even in presence of high expressing mutant genotype was observed and needs to be investigated in context of the pool of activated cells producing the cytokine.
authors.affiliation: Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur 784028, Assam, India.
authors.forename: Anusree
authors.initials: A
authors.lastname: Mahanta
authors.affiliation: Department of Medicine, Assam Medical College & Hospital, Lila Gogoi Path, Dibrugarh 786002, Assam, India.
authors.forename: Sanjeeb
authors.initials: S
authors.lastname: Kakati
authors.affiliation: Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur 784028, Assam, India. Electronic address: sbaruah@tezu.ernet.in.
authors.forename: Shashi
authors.initials: S
authors.lastname: Baruah
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002648:Child; D003710:Demography; D005260:Female; D005334:Fever / Q000235:genetics; D005786:Gene Expression Regulation; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D007194:India; D016209:Interleukin-8 / Q000235:genetics*; D008288:Malaria / Q000150:complications* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D053773:Transforming Growth Factor beta1 / Q000235:genetics; D055815:Young Adult
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8; D053773:Transforming Growth Factor beta1
keywords: ARMS–PCR; ASPR; CI; Complicated malaria; Cytokine; OR; Pf; Plasmodium falciparum; SNP; Single nucleotide polymorphism; amplification refractory mutational system–polymerase chain reaction; annual slide positivity rate; confidence interval; odds ratio; single nucleotide polymorphism