title: The temporary dynamics of inflammation-related genes expression under tuftsin analog Selank action.
abstract: Previous studies have shown that synthetic tuftsin analogue Selank and its fragments cause a number of alterations in the expression of certain genes involved in inflammation in mouse spleen. In this work we studied the effect of Selank and its short fragment Gly-Pro on the temporary dynamics of C3, Casp1, Il2rg, and Xcr1 genes expression in mouse spleen after single intraperitoneal injection (100 μg/kg) of peptides using real-time PCR method. We found a significant 3-fold decrease in the C3 mRNA level just 30 min after Selank injection and similar alteration this gene mRNA level after Gly-Pro administration. A wave-like alteration in the Casp1 mRNA level was observed after Selank injection. We found a significant alteration in the mRNA level of the Il2rg gene at early time points after Selank and Gly-Pro administration and an almost equal reduction in the Xcr1 mRNA level 90 min after the administration of Selank and its fragment. Our results showed that, Selank and its short fragment Gly-Pro influence the expression of genes that mediate different types of immune responses, thereby maintaining the balance of the immune system. It should be noted that in most cases, there was a coincidence in the expression profiles of the studied genes after Selank and Gly-Pro administration. This might indicate an active contribution of the dipeptide to the final effect of Selank.
authors.affiliation: The Department of Molecular Basis of Human Genetics, Institute of Molecular Genetics Russian Academy of Sciences, 2 Kurchatov Sq., Moscow 123182, Russia. Electronic address: kotimur@img.ras.ru.
authors.forename: Timur
authors.initials: T
authors.lastname: Kolomin
authors.affiliation: The Department of Biomedical and Pharmaceutical Technology, Faculty of Biotechnology and Organic Synthesis, Lomonosov Moscow University of Fine Chemical Technology, 86 Vernadskogo Av, Moscow 119571, Russia. Electronic address: feriste@mail.ru.
authors.forename: Marina
authors.initials: M
authors.lastname: Morozova
authors.affiliation: The Department of Molecular Basis of Human Genetics, Institute of Molecular Genetics Russian Academy of Sciences, 2 Kurchatov Sq., Moscow 123182, Russia. Electronic address: anastas.volkova@gmail.com.
authors.forename: Anastasiya
authors.initials: A
authors.lastname: Volkova
authors.affiliation: The Department of Molecular Basis of Human Genetics, Institute of Molecular Genetics Russian Academy of Sciences, 2 Kurchatov Sq., Moscow 123182, Russia. Electronic address: shadrina@img.ras.ru.
authors.forename: Maria
authors.initials: M
authors.lastname: Shadrina
authors.affiliation: The Department of Chemistry of Physiologically Active Compounds, Institute of Molecular Genetics Russian Academy of Sciences, 2 Kurchatov Sq., Moscow 123182, Russia. Electronic address: landr@img.ras.ru.
authors.forename: Lyudmila
authors.initials: L
authors.lastname: Andreeva
authors.affiliation: The Department of Molecular Basis of Human Genetics, Institute of Molecular Genetics Russian Academy of Sciences, 2 Kurchatov Sq., Moscow 123182, Russia. Electronic address: slomin@img.ras.ru.
authors.forename: Petr
authors.initials: P
authors.lastname: Slominsky
authors.affiliation: The Department of Molecular Basis of Human Genetics, Institute of Molecular Genetics Russian Academy of Sciences, 2 Kurchatov Sq., Moscow 123182, Russia. Electronic address: limbor@img.ras.ru.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Limborska
authors.affiliation: The Department of Chemistry of Physiologically Active Compounds, Institute of Molecular Genetics Russian Academy of Sciences, 2 Kurchatov Sq., Moscow 123182, Russia. Electronic address: nfm@img.ras.ru.
authors.forename: Nikolay
authors.initials: N
authors.lastname: Myasoedov
mesh_terms: D000818:Animals; D020170:Caspase 1 / Q000235:genetics / Q000378:metabolism; D003176:Complement C3 / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007249:Inflammation / Q000235:genetics* / Q000276:immunology; D053631:Interleukin Receptor Common gamma Subunit / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D009842:Oligopeptides / Q000494:pharmacology*; D019707:Receptors, Chemokine / Q000235:genetics / Q000378:metabolism; D013154:Spleen / Q000276:immunology; D014405:Tuftsin / Q000031:analogs & derivatives
chemical_list: D003176:Complement C3; C507942:Il2rg protein, mouse; D053631:Interleukin Receptor Common gamma Subunit; D009842:Oligopeptides; D019707:Receptors, Chemokine; C551408:XC chemokine receptor 1, mouse; D020170:Caspase 1; D014405:Tuftsin; C100827:TP 7
keywords: Gly-Pro; Regulatory peptides; Selank; Transcriptome; Tuftsin