title: Transabdominal coelocentesis as early source of fetal DNA for chromosomal and molecular diagnosis.
abstract: This study reports a comparative analysis between results of transabdominal coelocentesis and traditional invasive procedure in order to assess the usefulness of coelocentesis as a source of fetal DNA for molecular and chromosomal analysis. A number of 28 women were included in the study. A successful sampling of coelomic fluid was obtained in 25 women by transabdominal procedure. A positive amplification of DNA with QF-PCR techniques was obtained in 90% of cases, while 10% of cases failed to reveal interpretable results. Although all samples were cultured, the growth rate was not sufficient to determine karyotypes within 2 weeks. Five samples were selected to be analyzed by array-based comparative genomic hybridization (a-CGH) but the interpretation of these results was difficult and ambiguous. Our results suggest that transabdominal coelocentesis is suitable for the detection of single DNA variation and for QF-PCR analysis, while further experiments are needed to develop optimized protocols for traditional karyotyping and array-analysis.
authors.affiliation: Clinical Department of Surgery, Division of Gynecology and Prenatal Diagnosis, Tor Vergata University Hospital , Rome , Italy .
authors.forename: Adalgisa
authors.initials: A
authors.lastname: Pietropolli
authors.forename: Roberta
authors.initials: R
authors.lastname: Vicario
authors.forename: Cristina
authors.initials: C
authors.lastname: Peconi
authors.forename: Stefania
authors.initials: S
authors.lastname: Zampatti
authors.forename: Maria Chiara
authors.initials: MC
authors.lastname: Quitadamo
authors.forename: Maria Vittoria
authors.initials: MV
authors.lastname: Capogna
authors.forename: Michele
authors.initials: M
authors.lastname: Ragazzo
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Nardone
authors.forename: Diana
authors.initials: D
authors.lastname: Postorivo
authors.forename: Paola
authors.initials: P
authors.lastname: Spitalieri
authors.forename: Simona
authors.initials: S
authors.lastname: Sarta
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Ratto
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Novelli
authors.forename: Federica
authors.initials: F
authors.lastname: Sangiuolo
authors.forename: Emilio
authors.initials: E
authors.lastname: Piccione
authors.forename: Emiliano
authors.initials: E
authors.lastname: Giardina
mesh_terms: D000328:Adult; D055028:Comparative Genomic Hybridization; D005260:Female; D005820:Genetic Testing; D006801:Humans; D019152:Paracentesis / Q000379:methods*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D011446:Prospective Studies; D055815:Young Adult
keywords: Array-based comparative genomic hybridization; QF-PCR; coelomic fluid; fetal DNA; transabdominal coelocentesis