title: BORIS/CTCFL is an RNA-binding protein that associates with polysomes.
abstract: BACKGROUND
  BORIS (CTCFL), a paralogue of the multifunctional and ubiquitously expressed transcription factor CTCF, is best known for its role in transcriptional regulation. In the nucleus, BORIS is particularly enriched in the nucleolus, a crucial compartment for ribosomal RNA and RNA metabolism. However, little is known about cytoplasmic BORIS, which represents the major pool of BORIS protein.
  
  
  RESULTS
  We show, firstly, that BORIS has a putative nuclear export signal in the C-terminal domain. Furthermore, BORIS associates with mRNA in both neural stem cells and young neurons. The majority of the BORIS-associated transcripts are different in the two cell types. Finally, by using polysome profiling we show that BORIS is associated with actively translating ribosomes.
  
  
  CONCLUSIONS
  We have demonstrated the RNA binding properties of cellular BORIS and its association with actively translating ribosomes. We suggest that BORIS is involved in gene expression at both the transcriptional and post-transcriptional levels.
authors.forename: Babatunji W
authors.initials: BW
authors.lastname: Ogunkolade
authors.forename: Tania A
authors.initials: TA
authors.lastname: Jones
authors.forename: Johan
authors.initials: J
authors.lastname: Aarum
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Szary
authors.forename: Nicholas
authors.initials: N
authors.lastname: Owen
authors.forename: Diego
authors.initials: D
authors.lastname: Ottaviani
authors.forename: Muhammad A
authors.initials: MA
authors.lastname: Mumin
authors.forename: Shyam
authors.initials: S
authors.lastname: Patel
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Pieri
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Silver
authors.affiliation: Centre for Neuroscience and Trauma, Queen Mary University of London, Blizard Institute, Barts and the London School of Medicine and Dentistry, London, E1 2AT, UK. d.sheer@qmul.ac.uk.
authors.forename: Denise
authors.initials: D
authors.lastname: Sheer
mesh_terms: D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D002466:Cell Nucleolus / Q000235:genetics* / Q000378:metabolism; D003593:Cytoplasm / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D011132:Polyribosomes / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis; D021382:Protein Sorting Signals; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012335:RNA, Ribosomal / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D014158:Transcription, Genetic
chemical_list: C456540:CTCFL protein, human; D004268:DNA-Binding Proteins; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D012335:RNA, Ribosomal