title: High performance multi-spectral interrogation for surface plasmon resonance imaging sensors.
abstract: Surface plasmon resonance (SPR) sensing has proven to be a valuable tool in the field of surface interactions characterization, especially for biomedical applications where label-free techniques are of particular interest. In order to approach the theoretical resolution limit, most SPR-based systems have turned to either angular or spectral interrogation modes, which both offer very accurate real-time measurements, but at the expense of the 2-dimensional imaging capability, therefore decreasing the data throughput. In this article, we show numerically and experimentally how to combine the multi-spectral interrogation technique with 2D-imaging, while finding an optimum in terms of resolution, accuracy, acquisition speed and reduction in data dispersion with respect to the classical reflectivity interrogation mode. This multi-spectral interrogation methodology is based on a robust five parameter fitting of the spectral reflectivity curve which enables monitoring of the reflectivity spectral shift with a resolution of the order of ten picometers, and using only five wavelength measurements per point. In fine, such multi-spectral based plasmonic imaging system allows biomolecular interaction monitoring in a linear regime independently of variations of buffer optical index, which is illustrated on a DNA-DNA model case.
authors.affiliation: Laboratoire Charles Fabry, Institut d'Optique Graduate School, University Paris Sud, CNRS, 2 avenue Augustin Fresnel, 91127 Palaiseau Cedex, France; Horiba Scientific, Avenue de la Vauve, Passage Jobin Yvon, CS 45002-91120 Palaiseau, France. Electronic address: alexandra.sereda@institutoptique.fr.
authors.forename: A
authors.initials: A
authors.lastname: Sereda
authors.affiliation: Laboratoire Charles Fabry, Institut d'Optique Graduate School, University Paris Sud, CNRS, 2 avenue Augustin Fresnel, 91127 Palaiseau Cedex, France.
authors.forename: J
authors.initials: J
authors.lastname: Moreau
authors.affiliation: Laboratoire Charles Fabry, Institut d'Optique Graduate School, University Paris Sud, CNRS, 2 avenue Augustin Fresnel, 91127 Palaiseau Cedex, France.
authors.forename: M
authors.initials: M
authors.lastname: Canva
authors.affiliation: Horiba Scientific, Avenue de la Vauve, Passage Jobin Yvon, CS 45002-91120 Palaiseau, France.
authors.forename: E
authors.initials: E
authors.lastname: Maillart
mesh_terms: D004247:DNA / Q000032:analysis / Q000378:metabolism*; D004867:Equipment Design; D009693:Nucleic Acid Hybridization; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods
chemical_list: D004247:DNA
keywords: Biosensor; DNA; Data dispersion; Multi-spectral imaging; Real-time measurement; Surface plasmon resonance