title: Circulating microRNAs and kallikreins before and after radical prostatectomy: are they really prostate cancer markers?
abstract: The aim of our study was to monitor serum levels of two miRNAs (miR-21 and miR-141) and three KLKs (hK3/PSA, hK11, and hK13) before and 1, 5, and 30 days after radical prostatectomy, in order to characterize their fluctuations after surgery. 38 patients with prostate cancer were included. miR-21 and miR-141 were quantified through real-time PCR, while ELISA assays were used to quantify hK3 (PSA), hK11, and hK13. Both miR-21 and miR-141 showed a significant increase at the 5th postoperative day, after which a gradual return to the preoperative levels was recorded. These findings suggest that miR-21 and miR-141 could be involved in postsurgical inflammatory processes and that radical prostatectomy does not seem to alter their circulating levels. Postoperative serum kallikreins showed a significant decrease, highlighting the potential usefulness of kallikreins apart from PSA as potential prostate cancer markers.
authors.affiliation: Department of Medical-Surgical Sciences and Public Health, Faculty of Medicine and Surgery, University of Perugia, Loc. S. Andrea delle Fratte, 06156 Perugia, Italy.
authors.forename: Maria Giulia
authors.initials: MG
authors.lastname: Egidi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cochetti
authors.forename: Maria Rita
authors.initials: MR
authors.lastname: Serva
authors.forename: Gabriella
authors.initials: G
authors.lastname: Guelfi
authors.forename: Danilo
authors.initials: D
authors.lastname: Zampini
authors.forename: Luca
authors.initials: L
authors.lastname: Mechelli
authors.forename: Ettore
authors.initials: E
authors.lastname: Mearini
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D003710:Demography; D006801:Humans; D007610:Kallikreins / Q000097:blood*; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D017430:Prostate-Specific Antigen / Q000097:blood; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000601:surgery*; D012372:ROC Curve; D012697:Serine Endopeptidases / Q000097:blood
chemical_list: D014408:Biomarkers, Tumor; C533895:MIRN141 microRNA, human; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; C114880:trypsin-like serine protease; C470778:KLK13 protein, human; D007610:Kallikreins; D012697:Serine Endopeptidases; D017430:Prostate-Specific Antigen