title: Characterization of polyhydroxyalkanoate-producing bacteria isolated from sludge of commercial pig farms for producing methyl esters.
abstract: The objectives of this work were to isolate and characterize the polyhydroxyalkanoate (PHA) producing bacteria in enriched piggery sludge and make methyl esters from PHA for industrial applications. The strain ECAe24 isolated from piggery sludge with the highest PHA production was selected to produce PHA and then methyl ester by trans-esterification using glucose as substrate under mesophilic conditions. The final product after trans-esterification consisted of approximately 75.39% of fatty acid methyl ester and was identified as decanoic acid-3-hydroxy-methyl ester, octanoic acid-3-hydroxy-methyl ester, and some other contents. The novelty of this study is to use PHA-producing bacteria from piggery sludge to make fatty acid methyl esters which can be used as materials for producing biodiesel from piggery wastes.
authors.affiliation: Division of Applied Biology, Animal Technology Institute Taiwan, Chunan, Chinese Taiwan.
authors.forename: Chin-Feng
authors.initials: CF
authors.lastname: Chang
authors.forename: Li-Chun
authors.initials: LC
authors.lastname: Chen
authors.forename: Cheng-Jer
authors.initials: CJ
authors.lastname: Hsieh
authors.forename: Kai-Chun
authors.initials: KC
authors.lastname: Chang
authors.forename: Jung-Jeng
authors.initials: JJ
authors.lastname: Su
mesh_terms: D000818:Animals; D006799:Housing, Animal; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D012722:Sewage / Q000382:microbiology*; D013552:Swine
chemical_list: D054813:Polyhydroxyalkanoates; D012722:Sewage