title: Predicting detection limits of enzyme-linked immunosorbent assay (ELISA) and bioanalytical techniques in general.
abstract: The detection limit is one of the most important performance parameters for bioanalytical techniques. Here we present a generic method to estimate the detection limit of biomolecular assays based on a step-by-step analysis of the assay procedure. Enzyme-linked immunosorbent assay (ELISA) is used here as an example; however, much of the information presented in this article may be applied to other types of biomolecular assays and analytical techniques. A clear understanding of what affects the detection limit can help researchers to evaluate different bio-analytical techniques properly, and to design better strategies to optimize and achieve the best analytical performance.
authors.affiliation: NanoScience Technology Center, University of Central Florida, Orlando, FL 32826, USA. Qun.Huo@ucf.edu.
authors.forename: Shiyun
authors.initials: S
authors.lastname: Zhang
authors.forename: Alexa
authors.initials: A
authors.lastname: Garcia-D'Angeli
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Brennan
authors.forename: Qun
authors.initials: Q
authors.lastname: Huo
mesh_terms: D055910:Antibodies, Immobilized / Q000276:immunology; D000941:Antigens / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D057230:Limit of Detection
chemical_list: D055910:Antibodies, Immobilized; D000941:Antigens; D006735:Horseradish Peroxidase