title: Oxygen levels in thermoplastic microfluidic devices during cell culture.
abstract: We developed a computational model to predict oxygen levels in microfluidic plastic devices during cell culture. This model is based on experimental evaluation of oxygen levels. Conditions are determined that provide adequate oxygen supply to two cell types, hepatocytes and endothelial cells, either by diffusion through the plastic device, or by supplying a low flow rate of medium.
authors.affiliation: Singapore MIT Alliance for Research and Technology, BioSystems and Micromechanics, 1 CREATE Way, #04-13/14 Enterprise Wing, Singapore 138602, Singapore.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Ochs
authors.forename: Junichi
authors.initials: J
authors.lastname: Kasuya
authors.forename: Andrea
authors.initials: A
authors.lastname: Pavesi
authors.forename: Roger D
authors.initials: RD
authors.lastname: Kamm
mesh_terms: D002478:Cells, Cultured; D022781:Hepatocytes / Q000737:chemistry / Q000166:cytology; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D010100:Oxygen / Q000032:analysis*; D011108:Polymers / Q000737:chemistry; D013447:Sulfites / Q000737:chemistry
chemical_list: D011108:Polymers; D013447:Sulfites; D010100:Oxygen; C025026:sodium sulfite