title: Cellular delivery of peptide nucleic acids (PNAs).
abstract: Cellular delivery methods are a prerequisite for cellular studies with PNA. This chapter describes PNA cellular delivery using cell-penetrating peptide (CPP)-PNA conjugates and transfection of PNA-ligand conjugates mediated by cationic lipids. Furthermore, two endosomolytic procedures employing chloroquine treatment or photochemical internalization (PCI) for significantly improving PNA delivery efficacy are described.
authors.affiliation: Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Shiraishi
authors.forename: Peter E
authors.initials: PE
authors.lastname: Nielsen
mesh_terms: D001559:Benzhydryl Compounds / Q000737:chemistry; D001692:Biological Transport; D057846:Cell-Penetrating Peptides / Q000737:chemistry / Q000378:metabolism*; D002738:Chloroquine / Q000494:pharmacology; D019826:Cholic Acid / Q000737:chemistry; D011992:Endosomes / Q000187:drug effects / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D050356:Lipid Metabolism; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017319:Photosensitizing Agents / Q000494:pharmacology; D014162:Transfection
chemical_list: C030545:4-methylbenzhydrylamine resin; D001559:Benzhydryl Compounds; D057846:Cell-Penetrating Peptides; D020135:Peptide Nucleic Acids; D017319:Photosensitizing Agents; D002738:Chloroquine; D019826:Cholic Acid