title: Determination of three triterpene alcohols in rat plasma after oral administration of pollen of Brassica campestris based on the utilization of fetal bovine serum as surrogate matrix.
abstract: 24-Dehydropollinstanol (DEH), 24-methylene cholesterol (MET) and 31-norcycloartenol (NOR) are the functional triterpene alcohols of pollen of Brassica campestris. To study the pharmacokinetics of the above components of pollen of B. campestris in rats, a liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed. To avoid the interference of endogenous MET in rat plasma, fetal bovine serum (FBS) was selected as surrogate matrix and validated. Rat plasma was liquid-liquid extracted, then the chromatographic separation was conducted on a poroshell 120 SB C18 column (2.7μm, 2.1mm×50mm) at 38°C within 5.6min utilizing a gradient elution with a mobile phase consisting of (A) 0.1% formic acid in water and (B) 0.1% formic acid in methanol. The detection was performed on a triple quadrupole tandem mass spectrometer in multiple reaction monitoring (MRM) mode using positive atmospheric pressure chemical ionization (APCI). The method was validated over the concentration of 9.8-1560ng/ml; the inter-and-intra-day precisions (RSD %) were ≤7.8%, and the accuracies (RE %) were -5.3% to 12.2%, the extraction recovery ranged from 73.5% to 106.9% for all of these analytes, and no obvious matrix effect was observed. The developed method was applied successfully to study the pharmacokinetics of DEH, MET and NOR in rats after oral administration of pollen of B. campestris.
authors.affiliation: Laboratory of Pharmaceutical Analysis and Drug Metabolism, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, College of Pharmaceutical Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Shirui
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Laboratory of Pharmaceutical Analysis and Drug Metabolism, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, College of Pharmaceutical Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Department of Research and Development, Conba Pharmaceutical Co., Ltd., Hangzhou 310050, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Ye
authors.affiliation: Laboratory of Pharmaceutical Analysis and Drug Metabolism, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, College of Pharmaceutical Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Guangfa
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Research and Development, Conba Pharmaceutical Co., Ltd., Hangzhou 310050, China.
authors.forename: Ruwei
authors.initials: R
authors.lastname: Wang
authors.affiliation: Laboratory of Pharmaceutical Analysis and Drug Metabolism, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, College of Pharmaceutical Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Laboratory of Pharmaceutical Analysis and Drug Metabolism, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, College of Pharmaceutical Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Su
authors.initials: S
authors.lastname: Zeng
authors.affiliation: Laboratory of Pharmaceutical Analysis and Drug Metabolism, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, College of Pharmaceutical Science, Zhejiang University, Hangzhou 310058, China. Electronic address: hdjiang@zju.edu.cn.
authors.forename: Huidi
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000438:Alcohols / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D000818:Animals; D001937:Brassica / Q000737:chemistry*; D002417:Cattle; D004355:Drug Stability; D016014:Linear Models; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage*; D011058:Pollen / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D044967:Serum / Q000737:chemistry; D014315:Triterpenes / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics
chemical_list: D000438:Alcohols; D010936:Plant Extracts; D014315:Triterpenes
keywords: 24-Dehydropollinstanol; 24-Methylene cholesterol; 31-Norcycloartenol; LC–MS/MS; Pharmacokinetics; Pollen of Brassica campestris