title: Enhanced production of Fumigaclavine C in liquid culture of Aspergillus fumigatus under a two-stage process.
abstract: Fumigaclavine C (FC) produced by Aspergillus fumigatus is a conidiation associated ergot alkaloid with strong anti-inflammatory activity. However, its wide application has been severely limited by low FC production from submerged culture. In this work, a novel two-stage culture process by combining shake culture with static culture was proposed to enhance the production of FC. After the process optimization, the FC production reached 62.7 mg/L, which was significantly higher than ever report. For scaling up this new culture process, the gas-liquid interfacial area per unit volume (Agas-liq) was identified as the key factor. The results showed that in a combined stirred-static bioreactor system, a maximum FC production (58.97 mg/L) was obtained at an Agas-liq value of 1.30 cm(2)/mL. These results demonstrated that two-stage culture is an efficient strategy to enhance FC production and the information obtained will be useful to production of this powerful bioactive compound on a large scale.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China.
authors.forename: Yi-Xiang
authors.initials: YX
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China.
authors.forename: Ling-Yun
authors.initials: LY
authors.lastname: Yao
authors.affiliation: Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, PR China.
authors.forename: Rui-Hua
authors.initials: RH
authors.lastname: Jiao
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China. Electronic address: luyanhua@ecust.edu.cn.
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Lu
authors.affiliation: Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, PR China. Electronic address: rxtan@nju.edu.cn.
authors.forename: Ren-Xiang
authors.initials: RX
authors.lastname: Tan
mesh_terms: D001232:Aspergillus fumigatus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D002244:Carbon / Q000494:pharmacology; D004876:Ergot Alkaloids / Q000096:biosynthesis*; D026121:Indole Alkaloids; D007700:Kinetics; D007753:Laboratories; D013172:Spores, Fungal / Q000187:drug effects / Q000378:metabolism; D014364:Tryptophan / Q000378:metabolism
chemical_list: D004876:Ergot Alkaloids; D026121:Indole Alkaloids; C492995:fumigaclavine C; D002244:Carbon; D014364:Tryptophan
keywords: Combined stirred–static bioreactor system; Fumigaclavine C; Kinetic profile; Two-stage culture