title: Reactive oxygen species (ROS) protection via cysteine oxidation in the epidermal cornified cell envelope.
abstract: The outermost layer of our skin functions as a barrier to protect us from physical, chemical, and biological environmental insults. This protective function is mediated by the epidermal cornified cell envelope (CE) which serves both as a mechanical and permeability barrier. Recently we have discovered that the CE constitutes also a first-line antioxidant shield which relies greatly on cysteine residues in CE precursor proteins. Here we describe methods and protocols to study the cysteine-mediated antioxidant function of the CE at the level of the whole organ (the skin), individual cells (keratinocytes), or isolated proteins (SPRR family).
authors.affiliation: University of Leiden, Leiden, The Netherlands.
authors.forename: Wilbert P
authors.initials: WP
authors.lastname: Vermeij
authors.forename: Claude
authors.initials: C
authors.lastname: Backendorf
mesh_terms: D003545:Cysteine / Q000378:metabolism*; D000078404:Epidermal Cells; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D013058:Mass Spectrometry; D057054:Molecular Imaging / Q000379:methods*; D010084:Oxidation-Reduction; D059748:Proteolysis; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013194:Staining and Labeling; D014357:Trypsin / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D014357:Trypsin; D003545:Cysteine