title: Assessment of adenosine deaminase (ADA) activity and oxidative stress in patients with chronic tonsillitis.
abstract: To emphasize the effectiveness of adenosine deaminase (ADA) enzyme, which has important roles in the differentiation of lymphoid cells, and oxidative stress in patients with chronic tonsillitis. Serum and tissue samples were obtained from 25 patients who underwent tonsillectomy due to recurrent episodes of acute tonsillitis. In the control group, which also had 25 subjects, only serum samples were taken as obtaining tissue samples would not have been ethically appropriate. ADA enzyme activity, catalase (CAT), carbonic anhydrase (CA), nitric oxide (NO) and malondialdehyde (MDA) were measured in the serum and tissue samples of patients and control group subjects. The serum values of both groups were compared. In addition, the tissue and serum values of patients were compared. Serum ADA activity and the oxidant enzymes MDA and NO values of the patient group were significantly higher than those of the control group (p < 0.001), the antioxidant enzymes CA and CAT values of the patient group were significantly lower than those of the control group (p < 0.001). In addition, while CA, CAT and NO enzyme levels were found to be significantly higher in the tonsil tissue of the patient group when compared to serum levels (p < 0.05), there was no difference between tissue and serum MDA and ADA activity (p > 0.05). Elevated ADA activity may be effective in the pathogenesis of chronic tonsillitis both by impairing tissue structure and contributing to SOR formation.
authors.affiliation: Department of Otorhinolaryngology, Medical Faculty, Yuzuncu Yıl University, 65400, Van, Turkey, fatihgarca@hotmail.com.
authors.forename: Mehmet Fatih
authors.initials: MF
authors.lastname: Garca
authors.forename: Halit
authors.initials: H
authors.lastname: Demir
authors.forename: Mahfuz
authors.initials: M
authors.lastname: Turan
authors.forename: Nazım
authors.initials: N
authors.lastname: Bozan
authors.forename: Ahmet
authors.initials: A
authors.lastname: Kozan
authors.forename: Şeyda Bayel
authors.initials: ŞB
authors.lastname: Belli
authors.forename: Ayşe
authors.initials: A
authors.lastname: Arslan
authors.forename: Hakan
authors.initials: H
authors.lastname: Cankaya
mesh_terms: D000243:Adenosine Deaminase / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D002256:Carbonic Anhydrases / Q000378:metabolism*; D016022:Case-Control Studies; D002374:Catalase / Q000378:metabolism*; D002648:Child; D002908:Chronic Disease; D005260:Female; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D018384:Oxidative Stress; D014068:Tonsillectomy; D014069:Tonsillitis / Q000201:enzymology* / Q000378:metabolism / Q000601:surgery; D055815:Young Adult
chemical_list: D009569:Nitric Oxide; D008315:Malondialdehyde; D002374:Catalase; C548482:ADA protein, human; D000243:Adenosine Deaminase; D002256:Carbonic Anhydrases