title: On-chip magneto-immunoassay for Alzheimer's biomarker electrochemical detection by using quantum dots as labels.
abstract: Electrochemical detection of cadmium-selenide/zinc-sulfide (CdSe@ZnS) quantum dots (QDs) as labeling carriers in an assay for apolipoprotein E (ApoE) detection has been evaluated. The immunocomplex was performed by using tosylactivated magnetic beads as preconcentration platform into a flexible hybrid polydimethylsiloxane (PDMS)-polycarbonate (PC) microfluidic chip with integrated screen printed electrodes (SPE). All the immunoassay was performed in chip and in flow mode. The sensitive electrochemical detection was obtained by square wave anodic stripping voltammetry. ApoE was evaluated for its potential as biomarker for Alzheimer's disease detection, achieving a limit of detection (LOD) of ~12.5 ng mL(-1) with a linear range from 10 to 200 ng mL(-1) and high accuracy for diluted human plasma.
authors.affiliation: Nanobioelectronics and Biosensors Group, ICN2-Institut Catala de Nanociencia i Nanotecnologia, 08193 Bellaterra, Barcelona, Spain; Autonomous University of Barcelona, 08193 Bellaterra, Barcelona, Spain.
authors.forename: Mariana
authors.initials: M
authors.lastname: Medina-Sánchez
authors.affiliation: Nanobioelectronics and Biosensors Group, ICN2-Institut Catala de Nanociencia i Nanotecnologia, 08193 Bellaterra, Barcelona, Spain.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Miserere
authors.affiliation: Nanobioelectronics and Biosensors Group, ICN2-Institut Catala de Nanociencia i Nanotecnologia, 08193 Bellaterra, Barcelona, Spain; Polytechnic University of Catalonia, ESAII Department, 08028 Barcelona, Spain.
authors.forename: Eden
authors.initials: E
authors.lastname: Morales-Narváez
authors.affiliation: Nanobioelectronics and Biosensors Group, ICN2-Institut Catala de Nanociencia i Nanotecnologia, 08193 Bellaterra, Barcelona, Spain; Catalan Institution for Research and Advanced Studies (ICREA), 08010 Barcelona, Spain. Electronic address: arben.merkoci@icn.cat.
authors.forename: Arben
authors.initials: A
authors.lastname: Merkoçi
mesh_terms: D000544:Alzheimer Disease / Q000097:blood*; D001057:Apolipoproteins E / Q000097:blood*; D015374:Biosensing Techniques / Q000295:instrumentation; D019187:Cadmium Compounds / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation; D004867:Equipment Design; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D045663:Quantum Dots / Q000737:chemistry*; D018036:Selenium Compounds / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D001057:Apolipoproteins E; D019187:Cadmium Compounds; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; C058667:cadmium selenide; C031238:zinc sulfide
keywords: Alzheimer biomarker; Apolipoprotein E (ApoE); Electrochemistry; Magnetic beads; Microfluidic; Quantum dots