title: Effect of uracil on pullulan production by Aureobasidium pullulans CGMCC1234.
abstract: Effect of uracil on the pullulan production, biomass and uridine phosphorylase (UPase) activity was studied in this research. Uracil was found to enhance pullulan accumulation and the addition time of uracil was crucial to pullulan production. Pullulan yield of 49.07 g/L was achieved by adding 5mM uracil at 48 h, by comparison to 37.72 g/L obtained with the control. UPase activity could not be detected at early growth stage of Aureobasidium pullulans, but stimulated by added uracil at logarithmic phase and stationary phase. The time course study on the fermentation of pullulan demonstrates that pullulan production was not closely associated with biomass accumulation. Results indicate that the increased pullulan yield brought by uracil was correlated with UPase activity.
authors.affiliation: The State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Long
authors.initials: L
authors.lastname: Sheng
authors.forename: Guilan
authors.initials: G
authors.lastname: Zhu
authors.forename: Qunyi
authors.initials: Q
authors.lastname: Tong
mesh_terms: D001203:Ascomycota / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D005936:Glucans / Q000096:biosynthesis*; D007700:Kinetics; D014498:Uracil / Q000494:pharmacology*; D014543:Uridine Phosphorylase / Q000378:metabolism
chemical_list: D005936:Glucans; D014498:Uracil; C009109:pullulan; D014543:Uridine Phosphorylase
keywords: A. pullulans; ADP-glucose; ATP; Aureobasidium pullulans; DCW; PDA; Pullulan; UDP-glucose; UPase; Uracil; Uridine phosphorylase; adenosine diphosphate glucose; adenosine triphosphate; dry cell weight; potato dextrose agar; uridine diphosphate glucose; uridine phosphorylase