title: Targeting SHCBP1 inhibits cell proliferation in human hepatocellular carcinoma cells.
abstract: Src homology 2 domain containing (SHC) is a proto-oncogene which mediates cell proliferation and carcinogenesis in human carcinomas. Here, the SHC SH2-domain binding protein 1 (SHCBP1) was first established to be up-regulated in human hepatocellular carcinoma (HCC) tissues by array-base comparative genome hybridization (aCGH). Meanwhile, we examine and verify it by quantitative real-time PCR and western blot. Our current data show that SHCBP1 was up-regulated in HCC tissues. Overexpression of SHCBP1 could significantly promote HCC cell proliferation, survival and colony formation in HCC cell lines. Furthermore, knockdown of SHCBP1 induced cell cycle delay and suppressed cell proliferation. Furthermore, SHCBP1 could regulate the expression of activate extracellular signal-regulated kinase 1/2 (ERK1/2) and cyclin D1. Together, our findings indicate that SHCBP1 may contribute to human hepatocellular carcinoma by promoting cell proliferation and may serve as a molecular target of cancer therapy.
authors.affiliation: Department of Hepatobiliary Surgery, Wuxi Municipal People's Hospital of Nanjing Medical University, Wuxi, China E-mail : caibing@wuxiph.com, hanzg@chgc.sh.cn.
authors.forename: Han-Chuan
authors.initials: HC
authors.lastname: Tao
authors.forename: Hai-Xiao
authors.initials: HX
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Dai
authors.forename: Cheng-Yu
authors.initials: CY
authors.lastname: Gu
authors.forename: Qun
authors.initials: Q
authors.lastname: Wang
authors.forename: Ze-Guang
authors.initials: ZG
authors.lastname: Han
authors.forename: Bing
authors.initials: B
authors.lastname: Cai
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D019938:Cyclin D1 / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D020935:MAP Kinase Signaling System / Q000235:genetics; D000090063:Proto-Oncogene Mas; D055765:Shc Signaling Adaptor Proteins / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D055765:Shc Signaling Adaptor Proteins; D019938:Cyclin D1