title: Live cell visualization of Golgi membrane dynamics by super-resolution confocal live imaging microscopy.
abstract: Super-resolution confocal live imaging microscopy we developed provides cutting-edge high-speed live cell imaging at high space resolution. With this technology we are now able to observe details of membrane traffic events, including behaviors of small vesicles, cisternal maturation of the Golgi apparatus, and membrane segregation within a compartment.
authors.affiliation: Live Cell Molecular Imaging Research Team, Extreme Photonics Research Group, RIKEN Center for Advanced Photonics, Wako, Saitama, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kurokawa
authors.forename: Midori
authors.initials: M
authors.lastname: Ishii
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Suda
authors.forename: Akira
authors.initials: A
authors.lastname: Ichihara
authors.forename: Akihiko
authors.initials: A
authors.lastname: Nakano
mesh_terms: D006056:Golgi Apparatus / Q000378:metabolism*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000648:ultrastructure*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D059010:Single-Cell Analysis
chemical_list: D029701:Saccharomyces cerevisiae Proteins
keywords: Cisternal maturation; Live imaging; Multicolor visualization; Spinning disk confocal; Super-resolution