title: Bacterial community of biofilms developed under different water supply conditions in a distribution system.
abstract: In order to understand the bacterial community characteristics of biofilms developed under different finished water supply histories in drinking water distribution systems (DWDS), biofilm samples on different type of iron corrosion scales in a real DWDS were collected and systematically investigated using 454 pyrosequencing of 16S rRNA gene. The richness and diversity estimators showed that biofilms formed in DWDS transporting finished groundwater (GW) had the lowest level of bacterial diversity. From phylum to genus level, the dominant bacterial groups found in the biofilms under finished surface water (SW) and GW conditions were distinct. Proteobacteria was the dominant group in all biofilm samples (in the range of 40%-97%), but was relatively higher in biofilms with GW. The relative abundance of Firmicutes in biofilms with SW (28%-35%) was significantly higher (p<0.01) than that in biofilms with GW (0.5%-2.88%). Statistical analysis (Spearman's rank) revealed that alkalinity and chemical oxygen demand (CODMn) positively correlated with the relative abundance of Proteobacteria and Firmicutes, respectively. The abundance of sequences affiliated to iron-reducing bacteria (mainly Bacillus) and iron-oxidizing bacteria (mainly Acidovorax) were relatively higher in biofilms with SW, which might contribute to the formation of much thicker or tubercle-formed corrosion scales under SW supply condition. Several potential opportunistic pathogens, such as Burkholderia fungorum, Mycobacterium neoaurum, Mycobacterium frederiksbergense were detected in the biofilms.
authors.affiliation: State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, P.O. Box 2871, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, P.O. Box 2871, Beijing 100085, China. Electronic address: byshi@rcees.ac.cn.
authors.forename: Baoyou
authors.initials: B
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, P.O. Box 2871, Beijing 100085, China.
authors.forename: Yaohui
authors.initials: Y
authors.lastname: Bai
authors.affiliation: State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, P.O. Box 2871, Beijing 100085, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Wang
mesh_terms: D001419:Bacteria / Q000145:classification / Q000254:growth & development* / Q000378:metabolism; D018441:Biofilms; D003343:Corrosion; D060766:Drinking Water / Q000737:chemistry / Q000382:microbiology*; D060587:Groundwater / Q000737:chemistry / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D014871:Water Microbiology; D014881:Water Supply
chemical_list: D060766:Drinking Water
keywords: 454 pyrosequencing; Bacterial community; Biofilm; Corrosion scale; Drinking water distribution systems (DWDS); Water source