title: Ultraviolet B irradiation increases the expression of inflammatory cytokines in cultured sebocytes.
abstract: Sebaceous gland hyperplasia and increased sebum secretion after irradiation of ultraviolet (UV)-B has been widely accepted. This study was performed to clarify expression of inflammatory cytokines after irradiating UV-B in cultured sebocytes. Reverse transcription polymerase chain reaction was performed to measure gene expression of inflammatory cytokines, including interleukin (IL)-1β, IL-6, IL-8 and tumor necrosis factor-α, in cultured sebocytes after exposure to 40 and 70 mJ/cm(2) UV-B. Protein expression of inflammatory cytokines and lipid production in cultured sebocytes after exposure to UV-B were measured using enzyme-linked immunoassay and lipid analysis kit. The expression of inflammatory cytokines, especially IL-1β and IL-8, was significantly increased in cultured sebocytes after treatment with UV-B. Many more studies on the effect of UV-B on sebaceous glands should be performed to reveal the pathogenic mechanism of acne.
authors.affiliation: Department of Dermatology, Kyungpook National University School of Medicine, Daegu, Korea.
authors.forename: Weon Ju
authors.initials: WJ
authors.lastname: Lee
authors.forename: Kyung Hea
authors.initials: KH
authors.lastname: Park
authors.forename: Mi Yeung
authors.initials: MY
authors.lastname: Sohn
authors.forename: Won Chae
authors.initials: WC
authors.lastname: Lee
authors.forename: Seok-Jong
authors.initials: SJ
authors.lastname: Lee
authors.forename: Do Won
authors.initials: DW
authors.lastname: Kim
mesh_terms: D000152:Acne Vulgaris / Q000209:etiology*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D012627:Sebaceous Glands / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators
keywords: interleukin-1β; interleukin-8; sebocytes; ultraviolet B