title: Identification of a novel HIV Type 1 circulating recombinant form (CRF65_cpx) composed of CRF01_AE and subtypes B and C in Western Yunnan, China.
abstract: A novel HIV-1 circulating recombinant form (CRF) designated CRF65_cpx was recently characterized from three epidemiologically unlinked individuals infected through heterosexual contact in western Yunnan province of China. This is the first complex mosaic HIV-1 CRF, consisting of contributions from three or more different subtypes, identified in China. An additional full-length genome sequence with identical recombinant breakpoints was found among a previously reported recombinant strain from a man who had sex with a man in Anhui province of East Central China. The breakpoint analysis of the recombinants showed a complex genome organization composed of parental subtypes B' (Thailand variant of subtype B), C, and CRF01_AE, with 13 recombination breakpoints observed in almost all structure genes of HIV-1. The generation of complex recombinant forms is likely due to cocirculation of multiple lineages of HIV-1 strains in high-risk populations in western Yunnan.
authors.affiliation: 1 State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention , Chinese Center for Disease Control and Prevention, Beijing, China .
authors.forename: Yi
authors.initials: Y
authors.lastname: Feng
authors.forename: Huamian
authors.initials: H
authors.lastname: Wei
authors.forename: Jenny
authors.initials: J
authors.lastname: Hsi
authors.forename: Hui
authors.initials: H
authors.lastname: Xing
authors.forename: Xiang
authors.initials: X
authors.lastname: He
authors.forename: Lingjie
authors.initials: L
authors.lastname: Liao
authors.forename: Yanling
authors.initials: Y
authors.lastname: Ma
authors.forename: Chuanyi
authors.initials: C
authors.lastname: Ning
authors.forename: Nidan
authors.initials: N
authors.lastname: Wang
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Takebe
authors.forename: Yiming
authors.initials: Y
authors.lastname: Shao
mesh_terms: D000293:Adolescent; D002681:China; D016000:Cluster Analysis; D005260:Female; D023061:Gene Order; D016679:Genome, Viral; D005838:Genotype; D015658:HIV Infections / Q000821:virology; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012328:RNA Viruses / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D012367:RNA, Viral