title: Biodecolorization of a food azo dye by the deep sea Dermacoccus abyssi MT1.1(T) strain from the Mariana Trench.
abstract: This study reports the characterization of the ability of Dermacoccus spp. isolated from the deepest point of the world's oceans for azo dye decolorization. A detailed investigation of Dermacoccus abyssi MT1.1(T) with respect to the azoreductase activity and enzymatic mechanism as well as the potential role of the bacterial strain for biocleaning of industrial dye baths is reported. Resting cells with oxygen-insensitive azoreductase resulted in the rapid decolorization of the polysulfonated dye Brilliant Black BN (BBN) which is a common food colorant. The highest specific decolorization rate (vs) was found at 50 °C with a moderately thermal tolerance for over 1 h. Kinetic analysis showed the high rates and strong affinity of the enzymatic system for the dye with a Vmax = 137 mg/g cell/h and a Km = 19 mg/L. The degradation of BBN produces an initial orange intermediate, 8-amino-5-((4-sulfonatophenyl)diazenyl)naphthalene-2-sulfonic acid, identified by mass spectrometry which is later converted to 4-aminobenzene sulfonic acid. Nearly 80% of the maximum vs is possible achieved in resting cell treatment with the salinity increased up to 5.0% NaCl in reaction media. Therefore, this bacterial system has potential for dye decolorization bioprocesses occurring at high temperature and salt concentrations e.g. for cleaning dye-containing saline wastewaters.
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Weeranuch
authors.initials: W
authors.lastname: Lang
authors.affiliation: Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand; Centre for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University, Bangkok 10900, Thailand. Electronic address: sarote.s@ku.ac.th.
authors.forename: Sarote
authors.initials: S
authors.lastname: Sirisansaneeyakul
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras 2780-157, Portugal.
authors.forename: Lígia O
authors.initials: LO
authors.lastname: Martins
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Lukana
authors.initials: L
authors.lastname: Ngiwsara
authors.affiliation: Graduate School of Environmental Science, Hokkaido University, Sapporo 060-0810, Japan.
authors.forename: Nobuo
authors.initials: N
authors.lastname: Sakairi
authors.affiliation: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Wasu
authors.initials: W
authors.lastname: Pathom-aree
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Okuyama
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Haruhide
authors.initials: H
authors.lastname: Mori
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. Electronic address: kimura@abs.agr.hokudai.ac.jp.
authors.forename: Atsuo
authors.initials: A
authors.lastname: Kimura
mesh_terms: D000192:Actinomycetales / Q000378:metabolism*; D001391:Azo Compounds / Q000378:metabolism*; D004396:Coloring Agents / Q000378:metabolism*; D007700:Kinetics; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009601:Nitroreductases; D010140:Pacific Ocean; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D014877:Water Pollution, Chemical / Q000517:prevention & control*
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D014874:Water Pollutants, Chemical; C000298:brilliant black 1; D009247:NADH, NADPH Oxidoreductases; D009601:Nitroreductases; C021106:azoreductase
keywords: Azoreductase; Brilliant Black BN; Dermacoccus abyssi; Dye reduction; Mariana Trench