title: Formulation and evaluation of gemcitabine-loaded solid lipid nanoparticles.
abstract: Gemcitabine-loaded solid lipid nanoparticles (SLNs) were produced by double emulsification technique using stearic acid as lipid, soy lecithin as surfactant and sodium taurocholate as cosurfactant. Prepared nanoparticles are characterized for particle size and surface morphology using scanning electron microscopy (SEM). Particle yield, entrapment efficiency and zeta potential were also determined. In-vitro release studies were performed in phosphate-buffered saline (PBS) pH 7.4 using metabolic shaker. The formulation F6 with maximum entrapment efficiency 72.42% and satisfactory in-vitro release was selected. In-vivo tissue distribution to liver, spleen, lung, heart and kidneys of optimized formulation followed by stability study under specific conditions were also determined. This investigation has shown preferential drug targeting to liver followed by spleen, lungs, kidneys and heart. Stability studies showed no significant change in the particle size followed with very slight decrease in entrapment efficiency at 25 ± 2 °C/60 ± 5% RH over a period of three months.
authors.affiliation: KLEU College of Pharmacy , Belgaum, Karnataka , India and.
authors.forename: P T
authors.initials: PT
authors.lastname: Nandini
authors.forename: R C
authors.initials: RC
authors.lastname: Doijad
authors.forename: H N
authors.initials: HN
authors.lastname: Shivakumar
authors.forename: P M
authors.initials: PM
authors.lastname: Dandagi
mesh_terms: D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002626:Chemistry, Pharmaceutical; D003841:Deoxycytidine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D054709:Lecithins / Q000737:chemistry / Q000493:pharmacokinetics*; D008055:Lipids / Q000737:chemistry / Q000493:pharmacokinetics; D053758:Nanoparticles / Q000737:chemistry*; D051381:Rats; D013025:Glycine max; D013229:Stearic Acids / Q000737:chemistry / Q000493:pharmacokinetics*; D013656:Taurocholic Acid / Q000737:chemistry / Q000493:pharmacokinetics*; D000093542:Gemcitabine
chemical_list: D000964:Antimetabolites, Antineoplastic; D054709:Lecithins; D008055:Lipids; D013229:Stearic Acids; D003841:Deoxycytidine; C031183:stearic acid; D013656:Taurocholic Acid; D000093542:Gemcitabine
keywords: Entrapment efficiency; gemcitabine; in-vivo tissue distribution; solid lipid nanoparticles; stearic acid