title: Multielectrode bisensor system for time-resolved monitoring of ion transport across an epithelial cell layer.
abstract: An ion-selective multielectrode bisensor system is designed to ensure reliable real-time concentration measurements of sodium, potassium, chloride, and pH in a small volume of biological liquid bathing a living human bronchial epithelial cell monolayer. The bisensor system allows the monitoring of major ions, which are simultaneously transported through the epithelia in both directions.
authors.affiliation: Department of Biophysics, Warsaw University of Life Sciences - SGGW , Nowoursynowska 159, 02-776 Warszawa, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Toczyłowska-Mamińska
authors.forename: Andrzej
authors.initials: A
authors.lastname: Lewenstam
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Dołowy
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D017136:Ion Transport / Q000502:physiology*; D017736:Ion-Selective Electrodes; D008839:Microelectrodes; D013997:Time Factors