title: Positive prognostic impact of miR-210 in non-small cell lung cancer.
abstract: OBJECTIVE
  miR-210 is an important regulator of the cellular response to hypoxia. Therefore, we aimed to explore the prognostic significance of miR-210 in non-small cell lung cancer (NSCLC) patients with stage I-IIIA disease.
  
  
  METHODS
  In addition to clinicopathological and demograpic information, tumor tissues were collected and tissue micro arrays (TMAs) were constructed from 335 patients with stage I-IIIA NSCLC. Expression of miR-210 in cancer cells and stromal cells of the tumor was assessed by in situ hybridization.
  
  
  RESULTS
  In univariate analyses, high cancer cell (p=0.039) and high stromal cell expression (p=0.008) of miR-210 were both significantly associated with an improved disease-spesific survival (DSS). High co-expression of miR-210 in cancer and stromal cells was also a positive prognostic factor for DSS (p=0.010). In multivariate analysis, miR-210 in stromal cells (p=0.011), and miR-210 co-expressed in cancer and stromal cells was an independent prognosticator for DSS (p=0.011).
  
  
  CONCLUSIONS
  We show that miR-210 in stromal cells, and co-expressed in cancer cells and stromal cells mediates an independent prognostic impact. It is a candidate marker for prognostic stratification in NSCLC.
authors.affiliation: Department of Clinical Medicine, University of Tromso, Norway. Electronic address: marte.eilertsen@uit.no.
authors.forename: Marte
authors.initials: M
authors.lastname: Eilertsen
authors.affiliation: Department of Clinical Medicine, University of Tromso, Norway; Department of Oncology, University Hospital of North Norway, Tromso, Norway.
authors.forename: Sigve
authors.initials: S
authors.lastname: Andersen
authors.affiliation: Department of Medical Biology, University of Tromso, Norway; Department of Clinical Pathology, University Hospital of North Norway, Tromso, Norway.
authors.forename: Samer
authors.initials: S
authors.lastname: Al-Saad
authors.affiliation: Department of Medical Biology, University of Tromso, Norway; Department of Clinical Pathology, University Hospital of North Norway, Tromso, Norway.
authors.forename: Elin
authors.initials: E
authors.lastname: Richardsen
authors.affiliation: Department of Clinical Medicine, University of Tromso, Norway; Department of Oncology, University Hospital of North Norway, Tromso, Norway.
authors.forename: Helge
authors.initials: H
authors.lastname: Stenvold
authors.affiliation: Department of Clinical Medicine, University of Tromso, Norway.
authors.forename: Sigurd M
authors.initials: SM
authors.lastname: Hald
authors.affiliation: Department of Medical Biology, University of Tromso, Norway; Department of Pathology, Nordland Central Hospital, Bodo, Norway.
authors.forename: Khalid
authors.initials: K
authors.lastname: Al-Shibli
authors.affiliation: Department of Clinical Medicine, University of Tromso, Norway; Department of Oncology, University Hospital of North Norway, Tromso, Norway.
authors.forename: Tom
authors.initials: T
authors.lastname: Donnem
authors.affiliation: Department of Medical Biology, University of Tromso, Norway; Department of Clinical Pathology, University Hospital of North Norway, Tromso, Norway.
authors.forename: Lill-Tove
authors.initials: LT
authors.lastname: Busund
authors.affiliation: Department of Clinical Medicine, University of Tromso, Norway; Department of Oncology, University Hospital of North Norway, Tromso, Norway.
authors.forename: Roy M
authors.initials: RM
authors.lastname: Bremnes
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis* / Q000235:genetics / Q000401:mortality; D004847:Epithelial Cells / Q000502:physiology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D000860:Hypoxia / Q000235:genetics; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000401:mortality; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D017154:Stromal Cells / Q000502:physiology*; D016019:Survival Analysis; D046888:Tissue Array Analysis
chemical_list: C543800:MIRN210 microRNA, human; D035683:MicroRNAs
keywords: Biomarker; Cancer cells; NSCLC; Prognosis; Tumor stromal cells; miR-210