title: Enzymatic production of 15-hydroxyeicosatetraenoic acid from arachidonic acid by using soybean lipoxygenase.
abstract: 15-Hydroxyeicosatetraenoic acid (HETE), as a mammalian biologically active metabolite, has anticarcinogenic effect. The conditions of producing 15-HETE from arachidonic acid by using soybean lipoxygenase were optimal at pH 8.5 and 20°C with 9 g/l arachidonic acid, 54.4 U/ml soybean lipoxygenase, and 4% methanol. Under these optimized conditions, the enzyme produced 9.5 g/l 15-HETE after 25 min, with a molar conversion yield of 99% and a productivity of 22.8 g l(-1) h(-1). To the best of our knowledge, this is the first biotechnological production of 15-HETE.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Baek-Joong
authors.initials: BJ
authors.lastname: Kim
authors.forename: Kyung-Chul
authors.initials: KC
authors.lastname: Shin
authors.forename: Deok-Kun
authors.initials: DK
authors.lastname: Oh
mesh_terms: D016718:Arachidonic Acid / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods; D001711:Biotransformation; D006863:Hydrogen-Ion Concentration; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism*; D008084:Lipoxygenase / Q000378:metabolism*; D013025:Glycine max / Q000201:enzymology*; D013696:Temperature; D013997:Time Factors
chemical_list: D006893:Hydroxyeicosatetraenoic Acids; D016718:Arachidonic Acid; D008084:Lipoxygenase