title: Alpha-lipoic acid protects oxidative stress, changes in cholinergic system and tissue histopathology during co-exposure to arsenic-dichlorvos in rats.
abstract: We investigated protective efficacy of α-lipoic acid (LA), an antioxidant against arsenic and DDVP co-exposed rats. Biochemical variables suggestive of oxidative stress, neurological dysfunction, and tissue histopathological alterations were determined. Male rats were exposed either to 50 ppm sodium arsenite in drinking water or in combination with DDVP (4 mg/kg, subcutaneously) for 10 weeks. α-Lipoic acid (50mg/kg, pos) was also co-administered in above groups. Arsenic exposure led to significant oxidative stress along, hepatotoxicity, hematotoxicity and altered brain biogenic amines levels accompanied by increased arsenic accumulation in blood and tissues. These altered biochemical variables were supported by histopathological examinations leading to oxidative stress and cell death. These biochemical alterations were significantly restored by co-administration of α-lipoic acid with arsenic and DDVP alone and concomitantly. The results indicate that arsenic and DDVP induced oxidative stress and cholinergic dysfunction can be significantly protected by the supplementation of α-lipoic acid.
authors.affiliation: Division of Regulatory Toxicology, Defence Research and Development Establishment, Jhansi Road, Gwalior 474 002, India.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Dwivedi
authors.affiliation: Department of Applied Sciences, Sant Baba Bhag Singh Institute of Engineering and Technology, Jalandhar 144030, Panjab, India.
authors.forename: Govinder
authors.initials: G
authors.lastname: Flora
authors.affiliation: Division of Regulatory Toxicology, Defence Research and Development Establishment, Jhansi Road, Gwalior 474 002, India.
authors.forename: Pramod
authors.initials: P
authors.lastname: Kushwaha
authors.affiliation: Division of Regulatory Toxicology, Defence Research and Development Establishment, Jhansi Road, Gwalior 474 002, India. Electronic address: sjsflora@drde.drdo.in.
authors.forename: Swaran J S
authors.initials: SJ
authors.lastname: Flora
mesh_terms: D000109:Acetylcholine / Q000378:metabolism; D000110:Acetylcholinesterase / Q000097:blood; D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D018053:Arsenites / Q000097:blood / Q000493:pharmacokinetics / Q000633:toxicity*; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002800:Cholinesterase Inhibitors / Q000633:toxicity*; D004006:Dichlorvos / Q000633:toxicity*; D004347:Drug Interactions; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007958:Leukocyte Count; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010575:Pesticides / Q000633:toxicity*; D010976:Platelet Count; D051381:Rats; D017208:Rats, Wistar; D017670:Sodium Compounds / Q000097:blood / Q000493:pharmacokinetics / Q000633:toxicity*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D008063:Thioctic Acid / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D018053:Arsenites; D002800:Cholinesterase Inhibitors; D010575:Pesticides; D017670:Sodium Compounds; D017392:Thiobarbituric Acid Reactive Substances; C017947:sodium arsenite; D008063:Thioctic Acid; D004006:Dichlorvos; D007770:L-Lactate Dehydrogenase; D000410:Alanine Transaminase; D000110:Acetylcholinesterase; D000109:Acetylcholine
keywords: Arsenic; Brain biogenic amines; DDVP; Histopathology; Oxidative stress; α-Lipoic acid