title: The genome of the Lactobacillus sanfranciscensis temperate phage EV3.
abstract: BACKGROUND
  Bacteriophages infection modulates microbial consortia and transduction is one of the most important mechanism involved in the bacterial evolution. However, phage contamination brings food fermentations to a halt causing economic setbacks. The number of phage genome sequences of lactic acid bacteria especially of lactobacilli is still limited. We analysed the genome of a temperate phage active on Lactobacillus sanfranciscensis, the predominant strain in type I sourdough fermentations.
  
  
  RESULTS
  Sequencing of the DNA of EV3 phage revealed a genome of 34,834 bp and a G + C content of 36.45%. Of the 43 open reading frames (ORFs) identified, all but eight shared homology with other phages of lactobacilli. A similar genomic organization and mosaic pattern of identities align EV3 with the closely related Lactobacillus vaginalis ATCC 49540 prophage. Four unknown ORFs that had no homologies in the databases or predicted functions were identified. Notably, EV3 encodes a putative dextranase.
  
  
  CONCLUSIONS
  EV3 is the first L. sanfranciscensis phage that has been completely sequenced so far.
authors.affiliation: Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Gregor-Mendel-Str, 4, Freising 85354, Germany. m.ehrmann@wzw.tum.de.
authors.forename: Matthias A
authors.initials: MA
authors.lastname: Ehrmann
authors.forename: Angel
authors.initials: A
authors.lastname: Angelov
authors.forename: Claudia
authors.initials: C
authors.lastname: Picozzi
authors.forename: Roberto
authors.initials: R
authors.lastname: Foschino
authors.forename: Rudi F
authors.initials: RF
authors.lastname: Vogel
mesh_terms: D001435:Bacteriophages / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D016679:Genome, Viral; D007778:Lactobacillus / Q000821:virology*; D016366:Open Reading Frames; D010802:Phylogeny