title: A 43 kD light-regulated chloroplast RNA-binding protein interacts with the psbA 5' non-translated leader RNA.
abstract: Expression of the chloroplast psbA gene coding for the D1 protein of Photosystem II is subject to regulation at different levels in higher plants, including control of mRNA accumulation and translation. In dicots, the conserved 5' non-translated leader (5'-UTR) of the psbA mRNA is sufficient to direct the light-dependent translation of the D1 protein. In this report we show that the psbA mRNA 5'-UTR forms a stem-loop structure and binds a 43 kD chloroplast protein (43RNP). Binding of the 43RNP is sensitive to competition with poly(U), but insensitive to high concentrations of tRNA, the RNA homopolymers poly(A), poly(G), poly(C), or poly(A):poly(U) as a double-strand RNA. The 43RNP does not bind efficiently to the psbA mRNA 3' non-translated region, although the RNA sequence is U-rich and folds into a stem-loop. A deletion mutant of the psbA 5'-UTR RNA in which 5' sequences of the stem-loop are removed does not affect 43RNP binding. Together, these properties suggest that the 43RNP binds most effectively to a specific single-strand U-rich sequence preceding the AUG start codon in the psbA mRNA. Binding of the 43RNP is not detectable in plastid protein extracts from 5-day-old dark-grown seedlings, but is detectable in light-grown seedlings as well as mature plants in the light and after shifted to the dark. The 43RNP is therefore a candidate for a regulatory RNA-binding protein that may control the accumulation and/or translation of the psbA mRNA during light-dependent seedling development.
authors.affiliation: Institut für Physikalische Biologie, Heinrich-Heine-Universität, Universitätsstr. 1, D 40225, Düsseldorf, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Klaff
authors.forename: W
authors.initials: W
authors.lastname: Gruissem