title: Th17 and Th22 cells in psoriatic arthritis and psoriasis.
abstract: BACKGROUND
  The aim of this study was to characterize interleukin 17 (IL-17) and interleukin 22 (IL-22) producing cells in peripheral blood (PB), skin, synovial fluid (SF) and synovial tissue (ST) in patients with psoriasis (Ps) and psoriatic arthritis (PsA).
  
  
  METHODS
  Flow cytometry was used to enumerate cells making IL-22 and IL-17, in skin and/or SF and PB from 11 patients with Ps and 12 patients with PsA; skin and PB of 15 healthy controls and SF from rheumatoid arthritis (RA) patients were used as controls. Expression of the interleukin 23 receptor (IL-23R) and chemokine receptors CCR4 and CCR6 was examined. Secretion of IL-17 and IL-22 was measured by ELISA. ST was analysed by immunohistochemical staining of IL-17 and IL-22.
  
  
  RESULTS
  Increased frequencies of IL-17+ and IL-22+ CD4+ T cells were seen in PB of patients with PsA and Ps. IL-17 secretion was significantly elevated in both PsA and Ps, whilst IL-22 secretion was higher in PsA compared to Ps and healthy controls. A higher proportion of the CD4+ cells making IL-17 or IL-22 expressed IL-23R and frequencies of IL-17+, CCR6+ and CCR4+ T cells were elevated in patients with Ps and those with PsA. In patients with PsA, CCR6+ and IL-23R + T cells numbers were elevated in SF compared to PB. Increased frequencies of IL-17+ and IL-22+ CD4+ T cells were demonstrated in Ps skin lesions. In contrast, whilst elevated frequencies of CD4+ IL-17+ cells were seen in PsA SF compared to PB, frequencies of CD4+ IL-22+ T cells were lower. Whereas IL-17 expression was equivalent in PsA, osteoarthritis (OA) and RA ST, IL-22 expression was higher in RA than either OA or PsA ST, in which IL-22 was strikingly absent.
  
  
  CONCLUSIONS
  Elevated frequencies of IL-17 and IL-22 producing CD4+ T cells were a feature of both Ps and PsA. However their differing distribution at disease sites, including lower frequencies of IL-22+ CD4+ T cells in SF compared to skin and PB, and lack of IL-22 expression in ST suggests that Th17 and Th22 cells have common, as well as divergent roles in the pathogenesis of Ps and PsA.
authors.forename: Helen
authors.initials: H
authors.lastname: Benham
authors.forename: Paul
authors.initials: P
authors.lastname: Norris
authors.forename: Jane
authors.initials: J
authors.lastname: Goodall
authors.forename: Mihir D
authors.initials: MD
authors.lastname: Wechalekar
authors.forename: Oliver
authors.initials: O
authors.lastname: FitzGerald
authors.forename: Agnes
authors.initials: A
authors.lastname: Szentpetery
authors.forename: Malcolm
authors.initials: M
authors.lastname: Smith
authors.forename: Ranjeny
authors.initials: R
authors.lastname: Thomas
authors.forename: Hill
authors.initials: H
authors.lastname: Gaston
mesh_terms: D000328:Adult; D000368:Aged; D015535:Arthritis, Psoriatic / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D020381:Interleukin-17 / Q000097:blood / Q000134:cerebrospinal fluid / Q000276:immunology; D007378:Interleukins / Q000097:blood / Q000134:cerebrospinal fluid / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D054398:Receptors, CCR4 / Q000276:immunology / Q000378:metabolism; D054399:Receptors, CCR6 / Q000276:immunology / Q000378:metabolism; D018123:Receptors, Interleukin / Q000276:immunology / Q000378:metabolism; D012867:Skin / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D013583:Synovial Membrane / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D058504:Th17 Cells / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D000097043:Interleukin-22
chemical_list: D020381:Interleukin-17; D007378:Interleukins; D054398:Receptors, CCR4; D054399:Receptors, CCR6; D018123:Receptors, Interleukin; D000097043:Interleukin-22; C515354:CCR4 protein, human; C106311:CCR6 protein, human; C515500:IL23R protein, human