title: Multilectin-assisted fractionation for improved single-dot tissue glycome profiling in clinical glycoproteomics.
abstract: To survey the glycome shift in cancer, single-dot tissue glycome profiling was improved by incorporating a lectin-assisted fractionation. The differential analysis of tissue specimens from colorectal cancer patients (n = 45) revealed that unfucosylated/α2,6-sialylated glycoproteins significantly increased in patients with poor prognoses. The detailed annotation will be an indispensable supplement for cancer-related glyco-biomarker discovery.
authors.affiliation: Research Center for Medical Glycoscience (RCMG), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan. h.narimatsu@aist.go.jp.
authors.forename: Binbin
authors.initials: B
authors.lastname: Tan
authors.forename: Atsushi
authors.initials: A
authors.lastname: Matsuda
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kuno
authors.forename: Hisashi
authors.initials: H
authors.lastname: Narimatsu
mesh_terms: D014408:Biomarkers, Tumor; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D011379:Prognosis; D040081:Protein Array Analysis / Q000379:methods*; D040901:Proteomics
chemical_list: D014408:Biomarkers, Tumor; D006023:Glycoproteins; D037102:Lectins; D011134:Polysaccharides