title: Global gene expression of methicillin-resistant Staphylococcus aureus USA300 during human and mouse infection.
abstract: Little is known about the expression of methicillin-resistant Staphylococcus aureus (MRSA) genes during infection conditions. Here, we described the transcriptome of the clinical MRSA strain USA300 derived from human cutaneous abscesses, and compared it with USA300 bacteria derived from infected kidneys in a mouse model. Remarkable similarity between the transcriptomes allowed us to identify genes encoding multiple proteases and toxins, and iron- and peptide-transporter molecules, which are upregulated in both infections and are likely important for establishment of infection. We also showed that disruption of the global transcriptional regulators agr and sae prevents in vivo upregulation of many toxins and proteases, protecting mice from lethal infection dose, and hinting at the role of these transcriptional regulators in the pathology of MRSA infection.
authors.affiliation: Department of Bioinformatics & Computational Biology.
authors.forename: Shailesh V
authors.initials: SV
authors.lastname: Date
authors.forename: Zora
authors.initials: Z
authors.lastname: Modrusan
authors.forename: Michael
authors.initials: M
authors.lastname: Lawrence
authors.forename: J Hiroshi
authors.initials: JH
authors.lastname: Morisaki
authors.forename: Karen
authors.initials: K
authors.lastname: Toy
authors.forename: Ishita M
authors.initials: IM
authors.lastname: Shah
authors.forename: Janice
authors.initials: J
authors.lastname: Kim
authors.forename: Summer
authors.initials: S
authors.lastname: Park
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Li
authors.initials: L
authors.lastname: Basuino
authors.forename: Liana
authors.initials: L
authors.lastname: Chan
authors.forename: Deborah
authors.initials: D
authors.lastname: Zeitschel
authors.forename: Henry F
authors.initials: HF
authors.lastname: Chambers
authors.forename: Man-Wah
authors.initials: MW
authors.lastname: Tan
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Brown
authors.forename: Binh An
authors.initials: BA
authors.lastname: Diep
authors.forename: Wouter L W
authors.initials: WL
authors.lastname: Hazenbos
mesh_terms: D000038:Abscess / Q000382:microbiology; D000818:Animals; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D051379:Mice; D040081:Protein Array Analysis; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D017192:Skin Diseases, Bacterial / Q000382:microbiology; D059467:Transcriptome; D014774:Virulence
chemical_list: D012329:RNA, Bacterial
keywords: S. aureus exoprotein expression; accessory gene regulator; gene regulation; infection; methicillin-resistant Staphylococcus aureus (MRSA); transcriptome