title: Postnatal maternal separation enhances tonic GABA current of cortical layer 5 pyramidal neurons in juvenile rats and promotes genesis of GABAergic neurons in neocortical molecular layer and subventricular zone in adult rats.
abstract: Postnatal maternal separation (PMS) has been shown to be associated with an increased vulnerability to psychiatric illnesses in adulthood. However, the underlying neurological mechanisms are not well understood. Here we evaluated its effects on neurogenesis and tonic GABA currents of cortical layer 5 (L5) pyramidal neurons. PMS not only increased cell proliferation in the subventricular zone, cortical layer 1 and hippocampal dentate gyrus in the adult brain, but also promoted the newly generated cells to differentiate into GABAergic neurons, and PMS adult brain maintained higher ratios of GABAergic neurons in the survival of newly generated cells within 5 days immediately post PMS. Additionally, PMS increased the tonic currents at P7-10 and P30-35 in cortical L5 pyramidal cells. Our results suggest that the newly generated GABAergic neurons and the low GABA concentration-activated tonic currents may be involved in the development of psychiatric disorders after PMS.
authors.affiliation: Department of Neurology, The Children's Hospital Zhejiang University School of Medicine, 57 Zhugan Lane, Yanan Road, Hangzhou 310003, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Feng
authors.affiliation: Department of Neurology, The Children's Hospital Zhejiang University School of Medicine, 57 Zhugan Lane, Yanan Road, Hangzhou 310003, China.
authors.forename: Guoxia
authors.initials: G
authors.lastname: Sheng
authors.affiliation: Department of Neurology, The Children's Hospital Zhejiang University School of Medicine, 57 Zhugan Lane, Yanan Road, Hangzhou 310003, China.
authors.forename: Zhongxia
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of rehabilitation, The Children's Hospital Zhejiang University School of Medicine, 57 Zhugan Lane, Yanan Road, Hangzhou 310003, China.
authors.forename: Jiangping
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurology, The Children's Hospital Zhejiang University School of Medicine, 57 Zhugan Lane, Yanan Road, Hangzhou 310003, China.
authors.forename: Keming
authors.initials: K
authors.lastname: Ren
authors.affiliation: Stark Neurosciences Research Institute Indiana University School of Medicine, 980 W, Walnut Street, R3, Room C432A, Indianapolis, IN 46202, USA.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Jin
authors.affiliation: Department of Neurology, The Children's Hospital Zhejiang University School of Medicine, 57 Zhugan Lane, Yanan Road, Hangzhou 310003, China; Department of Laboratory, The Children's Hospital Zhejiang University School of Medicine, 57 Zhugan Lane, Yanan Road, Hangzhou 310003, China. Electronic address: kewenjiang@gmail.com.
authors.forename: Kewen
authors.initials: K
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001007:Anxiety / Q000503:physiopathology; D001921:Brain / Q000166:cytology / Q000254:growth & development* / Q000502:physiology*; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology / Q000254:growth & development / Q000502:physiology; D018891:Dentate Gyrus / Q000166:cytology / Q000254:growth & development / Q000502:physiology; D004644:Emotions / Q000502:physiology; D005260:Female; D059330:GABAergic Neurons / Q000166:cytology / Q000502:physiology*; D006624:Hippocampus / Q000166:cytology / Q000254:growth & development / Q000502:physiology; D066298:In Vitro Techniques; D008297:Male; D008426:Maternal Deprivation; D058953:Neural Stem Cells / Q000166:cytology / Q000502:physiology; D055495:Neurogenesis / Q000502:physiology*; D017966:Pyramidal Cells / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D005680:gamma-Aminobutyric Acid
keywords: Cell fate decision; Cell proliferation; Postnatal maternal separation; Tonic GABA current