title: Virus-induced gene complementation in tomato.
abstract: Virus-induced gene complementation (VIGC), a plant virus technology based on Potato virus X for transient overexpression of endogenous genes complemented tomato mutants, resulting in non-ripening fruits to ripen. This efficient "gain-of-function" approach involves no stable transformation, and reveals a fruit-specific transcriptional network that may exist among key transcription factors in modulating tomato ripening. Thus, VIGC represents a novel and feasible strategy for gene functional analysis in plants.
authors.affiliation: Research Centre for Plant RNA Signaling; College of Life and Environmental Sciences; Hangzhou Normal University; Hangzhou, PR China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Kong
authors.affiliation: Research Centre for Plant RNA Signaling; College of Life and Environmental Sciences; Hangzhou Normal University; Hangzhou, PR China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Research Centre for Plant RNA Signaling; College of Life and Environmental Sciences; Hangzhou Normal University; Hangzhou, PR China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Shen
authors.affiliation: Research Centre for Plant RNA Signaling; College of Life and Environmental Sciences; Hangzhou Normal University; Hangzhou, PR China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Qin
authors.affiliation: Research Centre for Plant RNA Signaling; College of Life and Environmental Sciences; Hangzhou Normal University; Hangzhou, PR China.
authors.forename: Tongfei
authors.initials: T
authors.lastname: Lai
authors.affiliation: Research Centre for Plant RNA Signaling; College of Life and Environmental Sciences; Hangzhou Normal University; Hangzhou, PR China.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Research Centre for Plant RNA Signaling; College of Life and Environmental Sciences; Hangzhou Normal University; Hangzhou, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Research Centre for Plant RNA Signaling; College of Life and Environmental Sciences; Hangzhou Normal University; Hangzhou, PR China.
authors.forename: Chaoqun
authors.initials: C
authors.lastname: Wu
authors.affiliation: School of Life Sciences; University of Warwick; Coventry, UK.
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.affiliation: Research Centre for Plant RNA Signaling; College of Life and Environmental Sciences; Hangzhou Normal University; Hangzhou, PR China; School of Life Sciences; University of Warwick; Coventry, UK.
authors.forename: Yiguo
authors.initials: Y
authors.lastname: Hong
mesh_terms: D017343:Genes, Plant; D005821:Genetic Techniques; D018551:Solanum lycopersicum / Q000235:genetics* / Q000254:growth & development / Q000821:virology*; D009154:Mutation / Q000235:genetics; D010940:Plant Proteins / Q000378:metabolism; D017863:Potexvirus / Q000502:physiology*
chemical_list: D010940:Plant Proteins
keywords: Potato virus X; VIGC; colourless non-ripening; functional complementation; plant virus technology; ripening inhibitor