title: Significance evaluation of the effects of environmental factors on the lipid accumulation of Chlorella minutissima UTEX 2341 under low-nutrition heterotrophic condition.
abstract: The effects of a variety of environmental factors on lipid accumulation of Chlorella minutissima UTEX 2341 under low-nutrition conditions were investigated. The growth of UTEX 2341 reached the exponential phase on the 1st day in low-nutrition medium. And the highest biomass productivity of 3.07 g L(-1) d(-1) was achieved on the 2nd day. The optimum pH and temperature for lipid accumulation were 6 and 20 °C respectively. 43.69 g L(-1) of NaCl and 0.11 mmol L(-1) of Fe(III) resulted in higher lipid content, analyzed by Design-Expert. And then under nitrogen starvation stress, the lipid content reached 22.84%. Meanwhile, the lipid yield was 2.5 g L(-1). The yield coefficient against carbon was 0.36 g g(-1), which was 4.68 times as much as that in OM medium. With the high proportion of C16 and C18 in the lipids, C. minutissima UTEX 2341 was proved to be a potential option for renewable biodiesel production.
authors.affiliation: State Key Laboratory of Agrobiotechnology, MOA Key Laboratory of Soil Microbiology and National Energy R & D Center for Non-food Biomass, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Agrobiotechnology, MOA Key Laboratory of Soil Microbiology and National Energy R & D Center for Non-food Biomass, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
authors.forename: HongLi
authors.initials: H
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Agrobiotechnology, MOA Key Laboratory of Soil Microbiology and National Energy R & D Center for Non-food Biomass, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
authors.forename: BaoZhen
authors.initials: B
authors.lastname: Li
authors.affiliation: State Key Laboratory of Agrobiotechnology, MOA Key Laboratory of Soil Microbiology and National Energy R & D Center for Non-food Biomass, College of Biological Sciences, China Agricultural University, Beijing 100193, China. Electronic address: yangjsh1999@163.com.
authors.forename: JinShui
authors.initials: J
authors.lastname: Yang
mesh_terms: D000704:Analysis of Variance; D018533:Biomass; D002244:Carbon / Q000494:pharmacology; D002708:Chlorella / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D004777:Environment; D052836:Heterotrophic Processes / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007501:Iron / Q000494:pharmacology; D008055:Lipids / Q000096:biosynthesis*; D008962:Models, Theoretical; D009584:Nitrogen / Q000494:pharmacology*; D015203:Reproducibility of Results; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects; D013696:Temperature
chemical_list: D008055:Lipids; D012965:Sodium Chloride; D002244:Carbon; D007501:Iron; D009584:Nitrogen
keywords: Biofuel; Central Composite Design; Environmental factors; Heterotrophic cultivation; Microalgae