title: Frequency of ace, epa and elrA Genes in Clinical and Environmental Strains of Enterococcus faecalis.
abstract: Surface proteins play an important role in the pathogenesis of enterococcal infections. Some of them are candidates for a vaccine, e.g., the frequency of endocarditis in rats vaccinated with Ace protein was 75 % as 12 opposed to 100 % in those who weren't. However, there are other components of enterococcal cells, such as Epa antigens or internalin-like proteins, which may be used in the prophylaxis of infections caused by them. However, also other virulence factors and resistance to antibiotics are important during enterococcal infection. Therefore, the relevance of ace, epa, elrA, other virulence genes, as well as resistance to antibiotics was investigated. 161 Enterococcus faecalis strains isolated from teaching hospitals in Lodz, cultured according to standard microbiological methods, were investigated for the presence of genes encoding surface proteins by PCR. Results were analyzed with χ(2) test. The elrA gene was found in all clinical and environmental strains, the ace gene was also widespread among E. faecalis (96.9 %). Both tested epa genes were found in the majority of isolates (83.25 %). There was correlation between the presence of esp and ace genes (p = 0.046) as well as between epa and agg genes (p = 0.0094; χ(2) test). The presence of the genes encoding surface proteins investigated in our study in the great majority of isolates implies that they would appear to be required during E. faecalis infection. Therefore, they could be excellent targets in therapy of enterococcal infections or, as some studies show, candidates for vaccines.
authors.affiliation: Medical and Sanitary Microbiology Department, Medical University of Lodz, Hallera Sq. 1, 90-647 Lodz, Poland.
authors.forename: Monika Eliza
authors.initials: ME
authors.lastname: Lysakowska
authors.forename: Andrzej
authors.initials: A
authors.lastname: Denys
authors.forename: Monika
authors.initials: M
authors.lastname: Sienkiewicz
keywords: Enterococcus faecalis; Virulence factors; ace; elrA; epa