title: The effect of alginate on DNA delivery from layer-by-layer assembled films.
abstract: Alginate has been previously utilized as an adjuvant in nanoparticle-mediated gene delivery. In this study, we investigated the extent to which alginate promotes in situ transfection from polyelectrolyte multilayers (PEMs). After spiking free alginate molecules, DNA release from PEMs was increased and transgene expression was enhanced, suggesting that alginate may compete with DNA to weaken electrostatic interaction within PEMs. Consequently, alginate was applied to compose multilayers with DNA and polyethyleneimine (PEI). Interestingly, the incorporated alginate increased not only deposition but also delivery of DNA from PEMs. Quartz crystal microbalance (QCM) analysis suggested that the deposited alginate enhanced PEI adsorption which in turn augmented subsequent DNA deposition. In situ transfection experiments revealed that alginate within PEMs improved not only the level but also the duration of transgene expression. This system should be a potential strategy to regulate gene delivery from scaffolds for tissue engineering application.
authors.affiliation: Department of Chemical and Materials Engineering, National Central University, Jhongli City, Taiwan. Electronic address: huweiwen@cc.ncu.edu.tw.
authors.forename: Wei-Wen
authors.initials: WW
authors.lastname: Hu
authors.forename: Shiang-Lung
authors.initials: SL
authors.lastname: Tsou
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004337:Drug Carriers / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D011094:Polyethyleneimine / Q000737:chemistry; D014162:Transfection
chemical_list: D000464:Alginates; D004337:Drug Carriers; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D011094:Polyethyleneimine; D004247:DNA
keywords: Alginate; Gene delivery; In situ transfection; Layer-by-layer assembly; Polyelectrolyte multilayer