title: Detection of T-cell receptor γ gene rearrangement in paraffin-embedded T or natural killer/T-cell lymphoma samples using the BIOMED-2 protocol.
abstract: While the use of polymerase chain reaction (PCR)-based clonality analysis of formalin-fixed paraffin-embedded (FFPE) tissue has recently become widespread, the detection sensitivity for lymphoma subtypes using FFPE samples is not well known. Here, we analyzed T-cell receptor γ chain (TCRG) gene rearrangement clonality in 100 cases of T- or natural killer (NK)/T-cell lymphoma and examined detection sensitivity according to lymphoma subtype. Clonality was detected in approximately 80% of the major T-cell lymphoma subtypes: peripheral T-cell lymphoma, not otherwise specified, 84% (21/25 cases); angioimmunoblastic T-cell lymphoma, 71% (15/21 cases); and adult T-cell leukemia/lymphoma, 80% (8/10 cases). The number of clonal peaks differed according to subtype. TCRG gene rearrangement was not detected in 63 cases of B-cell lymphoma or reactive lesions. Thus, clonality analysis can effectively and reliably detect TCRG gene rearrangement in T-cell lymphoma cases and could, therefore, be a useful diagnostic tool in routine practice.
authors.affiliation: Department of Pathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Miyata-Takata
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Takata
authors.forename: Sachiko
authors.initials: S
authors.lastname: Yamanouchi
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Sato
authors.forename: Mai
authors.initials: M
authors.lastname: Harada
authors.forename: Takashi
authors.initials: T
authors.lastname: Oka
authors.forename: Takehiro
authors.initials: T
authors.lastname: Tanaka
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Maeda
authors.forename: Mitsune
authors.initials: M
authors.lastname: Tanimoto
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yoshino
mesh_terms: D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics; D054391:Lymphoma, Extranodal NK-T-Cell / Q000175:diagnosis / Q000235:genetics*; D016399:Lymphoma, T-Cell / Q000175:diagnosis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
keywords: BIOMED-2; FFPE; T-cell receptor; sensitivity