title: Localized surface plasmon resonance based nanobiosensor for biomarker detection of invasive cancer cells.
abstract: In this study, we describe the development of a cancer biomarker-sensitive nanobiosensor based on localized surface plasmon resonance that enables recognition for proteolytic activity of membrane type 1 matrix metalloproteinase (MT1-MMP) anchored on invasive cancer cells. First of all, we prepared biomarker-detectable substrate based on gold nanorods (GNRs) using nanoparticle adsorption method. The sensitivity of the sensing chip was confirmed using various solvents that have different refractive indexes. Subsequently, MT1-MMP-specific cleavable peptide was conjugated onto the surface of GNRs, and molecular sensing about proteolytic activity was conducted using MT1-MMP and cell lysates. Collectively, we developed a biomarker detectable sensor, which allows for the effective detection of proteolytic activity about MT1-MMP extracted from invasive cancer cells.
authors.affiliation: Yonsei University, Department of Biomedical Engineering, Maeji 234, Heungup, Wonju, Gangwondo 220-710, Republic of Korea.
authors.forename: Yoochan
authors.initials: Y
authors.lastname: Hong
authors.forename: Minhee
authors.initials: M
authors.lastname: Ku
authors.forename: Eugene
authors.initials: E
authors.lastname: Lee
authors.forename: Jin-Suck
authors.initials: JS
authors.lastname: Suh
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Huh
authors.forename: Dae Sung
authors.initials: DS
authors.lastname: Yoon
authors.forename: Jaemoon
authors.initials: J
authors.lastname: Yang
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D006046:Gold / Q000737:chemistry; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D053511:Matrix Metalloproteinase 14 / Q000737:chemistry / Q000378:metabolism; D043942:Nanotubes / Q000737:chemistry; D009361:Neoplasm Invasiveness; D059748:Proteolysis; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D055767:Immobilized Proteins; D006046:Gold; D053511:Matrix Metalloproteinase 14