title: Biosynthesis of levan by levansucrase from Bacillus methylotrophicus SK 21.002.
abstract: A new strain SK 21.002 which could produce intracellular levansucrase was isolated from beet sugar growing soil. It was identified as Bacillus methylotrophicus based on the sequence analysis of the 16S rRNA and the morphological characteristic. The structure of levan biosynthesized from the levansucrase was determined to be connected through β-(2→6) linkages with fructofuranosyl residues. The molecular weight of the product was between 4 and 5 kDa. The optimal conditions to produce levan using the levansucrase were studied. The presence of Mg(2+) could increase the yield of levan. The yield of levan reached about 100g/L with the enzyme under the optimum conditions.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address: zhangtao@jiangnan.edu.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Runjing
authors.initials: R
authors.lastname: Li
authors.forename: Haobing
authors.initials: H
authors.lastname: Qian
authors.forename: Wanmeng
authors.initials: W
authors.lastname: Mu
authors.forename: Ming
authors.initials: M
authors.lastname: Miao
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
mesh_terms: D001407:Bacillus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001483:Base Sequence; D004305:Dose-Response Relationship, Drug; D005630:Fructans / Q000737:chemistry / Q000378:metabolism*; D006602:Hexosyltransferases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000494:pharmacology; D008969:Molecular Sequence Data; D013395:Sucrose / Q000494:pharmacology; D013696:Temperature
chemical_list: D005630:Fructans; D008670:Metals; D013395:Sucrose; C072599:levan; D006602:Hexosyltransferases; C021513:levansucrase
keywords: Bacillus methylotrophicus; Biosynthesis; Levan; Levansucrase