title: Fluorescence and HPLC Detection of Hydroxyl Radical by a Rhodamine-Nitroxide Probe and its Application in Cell Imaging.
abstract: A rhodamine nitroxide probe was designed to detect the hydroxyl radical (·OH), which presented high selectivity for ·OH over other reactive oxygen species (ROS) and linear fluorescence response to ·OH produced by Fenton reaction. The product was detected by HPLC-MS, indicating that the main product of the reaction was O-methylhydroxylamine and the product peak areas measured by HPLC-UV/vis and HPLC-FLD both enhanced proportionally with the increase of ·OH concentration. The application of the probe in biological system was explored to trace the production of ·OH in cells under oxidative stress condition induced by rotenone which can inhibit the mitochondria respiratory chain complex I and we found that appropriate rotenone may induce the normal human liver cells (L02) and human hepatoma cells (HepG2) to produce ·OH at different degrees.
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100039, People's Republic of China.
authors.forename: Linying
authors.initials: L
authors.lastname: Cao
authors.affiliation: Institute of Modern Physics Chinese Academy of Sciences, No.509 Nanchang Road, Lanzhou, 730000, People's Republic of China.
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Institute of Modern Physics Chinese Academy of Sciences, No.509 Nanchang Road, Lanzhou, 730000, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, People's Republic of China.
authors.forename: Shijun
authors.initials: S
authors.lastname: Shao
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, People's Republic of China. guoyong@lzb.ac.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Guo
mesh_terms: D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D017665:Hydroxyl Radical / Q000032:analysis*; D009589:Nitrogen Oxides / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D009589:Nitrogen Oxides; D012235:Rhodamines; D017665:Hydroxyl Radical
keywords: Fluorescence; Hydroxyl radical; Nitroxide; Oxidative stress; Spin label