title: Cloning and characterization of a potato StAN11 gene involved in anthocyanin biosynthesis regulation.
abstract: Anthocyanins are a class of products of plant secondary metabolism and are responsible for tubers color in potato. The biosynthesis of anthocyanins is a complex biological process, in which multiple genes are involved including structural genes and regulatory genes. In this study, StAN11, a WD40-repeat gene, was cloned from potato cultivar Chieftain (Solanum tuberosum L.). StAN11 (HQ599506) contained no intron and its open reading frame (ORF) was 1,029 bp long, encoding a putative protein of 342 amino acids. In order to verify its role in anthocyanin biosynthesis, StAN11 was inserted behind the CaMV-35S promoter of pCMBIA1304 and the recombination vector was introduced into the potato cultivar Désirée plants by Agrobacterium-mediated transformation. The color of transgenic tuber skin was significantly deepened, compared to the wild-type control, which was highly consistent with the accumulation of anthocyanin and expression of StAN11 in transgenic lines tuber skin. Further analysis on the expression of Flavonone-3-hydroxylase (F3H), Dihydroflavonol reductase (DFR), Anthocyanidin synthase (ANS), and Flavonoid 3-O-glucosyl transferase (3GT) in transgenic plants revealed that only DFR was upregulated. This result suggested that StAN11 regulated anthocyanin biosynthesis in potato by controlling DFR expression and accumulation of anthocyanin could be increased through overexpression of StAN11 in the tubers with the genetic background of anthocyanin biosynthesis.
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Li
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Man
authors.initials: M
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Jing-Ming
authors.initials: JM
authors.lastname: Yin
authors.forename: Ghullam Murtaza
authors.initials: GM
authors.lastname: Kaleri
authors.forename: Rui-Jie
authors.initials: RJ
authors.lastname: Zhang
authors.forename: Tie-Niu
authors.initials: TN
authors.lastname: Zuo
authors.forename: Xiong
authors.initials: X
authors.lastname: You
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
mesh_terms: D000872:Anthocyanins / Q000096:biosynthesis*; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D011198:Solanum tuberosum / Q000235:genetics / Q000378:metabolism*
chemical_list: D000872:Anthocyanins; D010940:Plant Proteins
keywords: Anthocyanin biosynthesis; StAN11; cloning genetic transformation; potato