title: SIRT1 Inhibits p53 but not NF-κB Transcriptional Activity during Differentiation of Mouse Embryonic Stem Cells into Embryoid Bodies.
abstract: OBJECTIVE
  SIRT1, a histone diacetylase, modify transactivation function of various transcription factor including p53 and NF-κB. p53 and NF-κB is involved in in vitro differentiation of mouse embryonic stem cells (mESC) into mouse embryoid body (mEB). These suggest that SIRT1 might affect in vitro differentiation of mESC into mEB by regulation of p53 and NF-κB.
  
  
  RESULTS
  In this study we analyzed the effect of SIRT1 in in vitro differentiation of mESC into mEB using wild and SIRT1 knockout mESC. To examine SIRT1-specific gene in mESC, this study conducted microarray-based differential gene expression analysis between wild and SIRT1 knockout mESC. Comparing their gene expression patterns, this study determined a list of genes regulated by SIRT1. cDNA microarray data-set analysis revealed that genes associated with transcription and signal transduction are significantly modified in SIRT1 knockout mESC. cDNA microarray data-set analysis between mESC and EB in wild and SIRT1 showed that SIRT1 inhibits p53 signaling pathway but not affect NF-κB signaling pathway.
  
  
  CONCLUSIONS
  This study suggests that SIRT1 modify mESC differentiation by regulation of p53 transcriptional activity.
authors.affiliation: Department of Microbiology, Chonbuk National University Medical School, Jeonju ; Department of Internal Medicine, Wonkwang University School of Medicine, Iksan, Korea.
authors.forename: Sang Jae
authors.initials: SJ
authors.lastname: Lhee
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Song
authors.forename: Yu-Ri
authors.initials: YR
authors.lastname: Kim
authors.forename: Myung-Kwan
authors.initials: MK
authors.lastname: Han
keywords: Embryoid bodies; Embryonic stem cells; NF-κB; SIRT1; p53