title: Modulation of matrix metalloproteinase-9 secretion from tumor-associated macrophage-like cells by proteolytically processed laminin-332 (laminin-5).
abstract: Macrophages infiltrating tumor tissues (tumor-associated macrophages, TAM) affect the malignant behaviors of tumor cells. We previously reported that monocytes were differentiated into TAM-like cells secreting matrix metalloproteinase (MMP)-9 by co-culture with tumor cells, and that cell adhesion to extracellular matrix (ECM) proteins played a critical role in the differentiation. In this study, we found that the monocyte differentiation was promoted by laminin-332 (laminin-5), a major epithelial ECM component. We also demonstrated that the proteolytic processing of the γ2 chain of laminin-332 was essential for its activity but that the N-terminal short arm of the γ2 chain inhibited MMP-9 secretion. These results indicate that the activity of laminin-332 for monocyte differentiation is dynamically regulated by the proteolytic processing of the γ2 chain.
authors.affiliation: Department of Microbiology, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo, 142-8501, Japan.
authors.forename: Go
authors.initials: G
authors.lastname: Kamoshida
authors.forename: Takashi
authors.initials: T
authors.lastname: Ogawa
authors.forename: Jun
authors.initials: J
authors.lastname: Oyanagi
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sato
authors.forename: Eriko
authors.initials: E
authors.lastname: Komiya
authors.forename: Shouichi
authors.initials: S
authors.lastname: Higashi
authors.forename: Kaoru
authors.initials: K
authors.lastname: Miyazaki
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics*; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008264:Macrophages / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D009000:Monocytes / Q000166:cytology*; D009369:Neoplasms / Q000235:genetics / Q000473:pathology; D000097794:Kalinin
chemical_list: D015815:Cell Adhesion Molecules; D016326:Extracellular Matrix Proteins; D020780:Matrix Metalloproteinase 9; D000097794:Kalinin