title: Protective effect of Millettia pulchra polysaccharide on cognitive impairment induced by D-galactose in mice.
abstract: A polysaccharide (PMP) was isolated from Millettia pulchra and purified by DEAE-cellulose and Sephadex G-75 chromatography. The results showed that PMP was composed of d-glucose and d-arabinose in a molar ratio of 90.79% and 9.21%, with an average molecular weight of about 14,301 Da. Furthermore, the effect of PMP on cognitive impairment induced by d-galactose in mice was evaluated. Treatment with PMP significantly reversed d-galactose-induced learning and memory impairments, as measured by behavioral tests. One of the potential mechanisms of this action was to reduce oxidative stress and suppress inflammatory responses. Furthermore, our results also showed that PMP markedly reduced the content and deposition of β-amyloid peptide, improved the dysfunction of synaptic plasticity, increased the levels of acetylcholine, but decreased cholinesterase activity. These results suggest that PMP exerts an effective protection against d-galactose-induced cognitive impairment, and PMP may be a major bioactive ingredient in M. pulchra.
authors.affiliation: Guangxi Medical University, Nanning, Guangxi 530021, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Lin
authors.forename: Zhongshi
authors.initials: Z
authors.lastname: Huang
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Chen
authors.forename: Yanping
authors.initials: Y
authors.lastname: Rong
authors.forename: Shijun
authors.initials: S
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Jiao
authors.forename: Quanfang
authors.initials: Q
authors.lastname: Huang
authors.forename: Renbin
authors.initials: R
authors.lastname: Huang
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000378:metabolism; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D002452:Cell Count; D003072:Cognition Disorders / Q000139:chemically induced* / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D004644:Emotions / Q000187:drug effects; D005690:Galactose / Q000009:adverse effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008124:Locomotion / Q000187:drug effects; D008297:Male; D008568:Memory / Q000187:drug effects; D051379:Mice; D033641:Millettia / Q000737:chemistry*; D009473:Neuronal Plasticity / Q000187:drug effects; D010446:Peptide Fragments / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D013037:Spatial Behavior / Q000187:drug effects / Q000502:physiology; D013569:Synapses / Q000187:drug effects
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D011134:Polysaccharides; C075222:amyloid beta-protein (1-42); D000110:Acetylcholinesterase; D005690:Galactose
keywords: Cognitive impairment; Millettia pulchra; Polysaccharide; d-Galactose