title: Bacteriocinogenic potential and safety evaluation of non-starter Enterococcus faecium strains isolated from home made white brine cheese.
abstract: Four LAB strains, isolated from Bulgarian home made white brine cheese, were selected for their effective inhibition against Listeria monocytogenes. According to their biochemical and physiological characteristics, the strains were classified as members of Enterococcus genus, and then identified as Enterococcus faecium by 16S rDNA sequencing. Their bacteriocin production and inhibitory spectrum were evaluated together with the occurrence of several bacteriocin genes (entA, entB, entP, entL50B). Their virulence potential and safety was assessed both using PCR targeted to the genes gelE, hyl, asa1, esp, cylA, efaA, ace, vanA, vanB, hdc1, hdc2, tdc and odc and by phenotypical tests for antibiotic resistance, gelatinase, lipase, DNAse and α- and β-haemolysis. The E. faecium strains harboured at least one enterocin gene while the occurrence of virulence, antibiotic resistance and biogenic amines genes was limited. Considering their strong antimicrobial activity against L. monocytogenes strains, the four E. faecium strains exhibited promising potential as bio-preservatives cultures for fermented food productions.
authors.affiliation: Department of Agronomy Food Natural resources Animals and Environment (DAFNAE), University of Padova, Agripolis, Viale dell'Università 16, 35020 Legnaro, PD, Italy. Electronic address: lorenzo.favaro@unipd.it.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Favaro
authors.forename: Marina
authors.initials: M
authors.lastname: Basaglia
authors.forename: Sergio
authors.initials: S
authors.lastname: Casella
authors.forename: Isabelle
authors.initials: I
authors.lastname: Hue
authors.forename: Xavier
authors.initials: X
authors.lastname: Dousset
authors.forename: Bernadette Dora
authors.initials: BD
authors.lastname: Gombossy de Melo Franco
authors.forename: Svetoslav Dimitrov
authors.initials: SD
authors.lastname: Todorov
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001430:Bacteriocins / Q000096:biosynthesis* / Q000494:pharmacology*; D002611:Cheese / Q000382:microbiology*; D016984:Enterococcus faecium / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008089:Listeria monocytogenes / Q000187:drug effects; D012492:Salts / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001430:Bacteriocins; D012492:Salts; C017082:brine
keywords: Anti-Listeria monocytogenes activity; Bacteriocin; Enterococcus faecium; Safety; Virulence; White brine cheese