title: RoKSN, a floral repressor, forms protein complexes with RoFD and RoFT to regulate vegetative and reproductive development in rose.
abstract: FT/TFL1 family members have been known to be involved in the development and flowering in plants. In rose, RoKSN, a TFL1 homologue, is a key regulator of flowering, whose absence causes continuous flowering. Our objectives are to functionally validate RoKSN and to explore its mode of action in rose. We complemented Arabidopsis tfl1 mutants and ectopically expressed RoKSN in a continuous-flowering (CF) rose. Using different protein interaction techniques, we studied RoKSN interactions with RoFD and RoFT and possible competition. In Arabidopsis, RoKSN complemented the tfl1 mutant by rescuing late flowering and indeterminate growth. In CF roses, the ectopic expression of RoKSN led to the absence of flowering. Different branching patterns were observed and some transgenic plants had an increased number of leaflets per leaf. In these transgenic roses, floral activator transcripts decreased. Furthermore, RoKSN was able to interact both with RoFD and the floral activator, RoFT. Protein interaction experiments revealed that RoKSN and RoFT could compete with RoFD for repression and activation of blooming, respectively. We conclude that RoKSN is a floral repressor and is also involved in the vegetative development of rose. RoKSN forms a complex with RoFD and could compete with RoFT for repression of flowering.
authors.affiliation: INRA, Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), SFR 4207 QUASAV, BP 60057, 49071, Beaucouzé Cedex, France.|Agrocampus Ouest, Institut de Recherche en Horticulture et Semences (INRA, Agrocampus-OUEST, Université d'Angers), SFR 4207 QUASAV, 2 rue Le Nôtre, 49045, Angers, France.|Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), Université d'Angers, SFR 4207 QUASAV, PRES LUNAM, BP 60057, 49071, Beaucouzé Cedex, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Randoux
authors.affiliation: Unité Propre de Recherche 2357, CNRS, Institut de Biologie Moléculaire des Plantes, Conventionné avec l'Université de Strasbourg, 12 rue du Général Zimmer, 67084, Strasbourg Cedex, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Davière
authors.affiliation: INRA, Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), SFR 4207 QUASAV, BP 60057, 49071, Beaucouzé Cedex, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Jeauffre
authors.affiliation: INRA, Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), SFR 4207 QUASAV, BP 60057, 49071, Beaucouzé Cedex, France.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Thouroude
authors.affiliation: INRA, Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), SFR 4207 QUASAV, BP 60057, 49071, Beaucouzé Cedex, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Pierre
authors.affiliation: INRA, Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), SFR 4207 QUASAV, BP 60057, 49071, Beaucouzé Cedex, France.
authors.forename: Youness
authors.initials: Y
authors.lastname: Toualbia
authors.affiliation: INRA, Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), SFR 4207 QUASAV, BP 60057, 49071, Beaucouzé Cedex, France.
authors.forename: Justine
authors.initials: J
authors.lastname: Perrotte
authors.affiliation: INRA, Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), SFR 4207 QUASAV, BP 60057, 49071, Beaucouzé Cedex, France.|DNM Plant Breeding, Institut Polytechnique - LaSalle Beauvais, 19 rue Pierre Waguet, BP 30313, 60026, Beauvais, France.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Reynoird
authors.affiliation: INRA, Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), SFR 4207 QUASAV, BP 60057, 49071, Beaucouzé Cedex, France.
authors.forename: Marie-José
authors.initials: MJ
authors.lastname: Jammes
authors.affiliation: INRA, Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), SFR 4207 QUASAV, BP 60057, 49071, Beaucouzé Cedex, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Hibrand-Saint Oyant
authors.affiliation: INRA, Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Université d'Angers), SFR 4207 QUASAV, BP 60057, 49071, Beaucouzé Cedex, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Foucher
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics; D035264:Flowers / Q000254:growth & development* / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D005816:Genetic Complementation Test; D056626:Inflorescence / Q000235:genetics / Q000254:growth & development; D009154:Mutation / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D012098:Reproduction; D027826:Rosa / Q000235:genetics / Q000254:growth & development*
chemical_list: D029681:Arabidopsis Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; C102864:TFL1 protein, Arabidopsis
keywords: FLOWERING LOCUS T (FT); PEBP family; TERMINAL FLOWER 1 (TFL1); flowering; polycarpic; rose; transgenic