title: The carboxyl-terminal region of the spinach PsaD subunit contains information for its specific assembly into plant thylakoids.
abstract: The assembly of the multi-subunit membrane-protein Photosystem I (PS I) complex involves incorporation of peripheral proteins into the complex. Here we studied assembly of the PsaD subunit of the cyanobacterial and plant PS I into the thylakoid membranes. We generated partial and chimeric psaD genes from which labeled proteins were synthesized in vitro. Assembly of these proteins into the cyanobacterial or plant thylakoids was assayed. The deletion of leader sequence and N-terminal extension of spinach prePsaD did not inhibit its assembly into spinach or cyanobacterial thylakoids. Addition of these sequences to the cyanobacterial PsaD did not enable it to assemble into plant thylakoids. Moreover, these additions significantly decreased the ability of the chimeric proteins to assemble into cyanobacterial thylakoids. In contrast, when the carboxyl-terminal half of cyanobacterial PsaD was replaced by the corresponding region of the spinach PsaD, the chimeric protein could assemble into both spinach and cyanobacterial thylakoids. Therefore, information in the carboxyl-terminal region of spinach PsaD is crucial for its assembly into plant thylakoids.
authors.affiliation: Botany Department, The Hebrew University of Jerusalem, 91904, Jerusalem, Israel.
authors.forename: Y
authors.initials: Y
authors.lastname: Cohen
authors.forename: N
authors.initials: N
authors.lastname: Nelson
authors.forename: P R
authors.initials: PR
authors.lastname: Chitnis
authors.forename: R
authors.initials: R
authors.lastname: Nechushtai