title: Effective determination of a pharmaceutical, sulpiride, in river water by online SPE-LC-MS using a molecularly imprinted polymer as a preconcentration medium.
abstract: We report an effective and a quantitative analysis method for one of pharmaceuticals, sulpiride, in river water by online solid phase extraction (SPE) connected with liquid chromatography-mass spectrometry (LC-MS) using a molecularly imprinted polymer as a preconcentration medium. The polymer prepared with a pseudo template molecule showed the selective retention ability based on the interval recognition of functional groups in sulpiride. Also, the imprinted polymer provided an effective concentration of a trace level of sulpiride in offline SPE with dual washing processes using water and acetonitrile, although another imprinted polymer prepared by an authentic method using sulpiride and methacrylic acid as a template and a functional monomer, respectively, showed the selective adsorption only in organic solvents. Furthermore, we employed the imprinted polymer as the preconcentration column of online SPE-LC-MS and the results supposed that the proposed system allowed the quantitative analysis of sulpiride with high sensitivity and recovery (10ng/L at 96%). Additionally, the determination of sulpiride in real river water without an additional spiking was effectively achieved by the system.
authors.affiliation: Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 6158510, Japan. Electronic address: kubo@anchem.mc.kyoto-u.ac.jp.
authors.forename: Takuya
authors.initials: T
authors.lastname: Kubo
authors.affiliation: Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 6158510, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Kuroda
authors.affiliation: Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 6158510, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Tominaga
authors.affiliation: Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 6158510, Japan.
authors.forename: Toyohiro
authors.initials: T
authors.lastname: Naito
authors.affiliation: Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai City, Osaka 5998531, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Sueyoshi
authors.affiliation: Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Shikogamo Hangicho, Kyoto 6068522, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Hosoya
authors.affiliation: Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 6158510, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Otsuka
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D005618:Fresh Water / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D054802:Molecular Imprinting / Q000379:methods; D011108:Polymers / Q000737:chemistry*; D045483:Rivers / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods; D012997:Solvents / Q000737:chemistry; D013469:Sulpiride / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000737:chemistry
chemical_list: D011108:Polymers; D012997:Solvents; D014874:Water Pollutants, Chemical; D013469:Sulpiride
keywords: Interval recognition; Molecular imprinting; Pharmaceutical; Solid phase extraction; Sulpiride