title: Functional divergence outlines the evolution of novel protein function in NifH/BchL protein family.
abstract: Biological nitrogen fixation is accomplished by prokaryotes through the catalytic action of complex metalloenzyme, nitrogenase. Nitrogenase is a two-protein component system comprising MoFe protein (NifD and K) and Fe protein (NifH). NifH shares structural and mechanistic similarities as well as evolutionary relationships with light-independent protochlorophyllide reductase (BchL), a photosynthesis-related metalloenzyme belonging to the same protein family. We performed a comprehensive bioinformatics analysis of the NifH/BchL family in order to elucidate the intrinsic functional diversity and the underlying evolutionary mechanism among the members. To analyse functional divergence in the NifH/ BchL family, we have conducted pair-wise estimation in altered evolutionary rates between the member proteins. We identified a number of vital amino acid sites which contribute to predicted functional diversity. We have also made use of the maximum likelihood tests for detection of positive selection at the amino acid level followed by the structure-based phylogenetic approach to draw conclusion on the ancient lineage and novel characterization of the NifH/BchL protein family. Our investigation provides ample support to the fact that NifH protein and BchL share robust structural similarities and have probably deviated from a common ancestor followed by divergence in functional properties possibly due to gene duplication.
authors.affiliation: NBU Bioinformatics Facility, Department of Botany, University of North Bengal, Siliguri, India.
authors.forename: Subarna
authors.initials: S
authors.lastname: Thakur
authors.forename: Asim K
authors.initials: AK
authors.lastname: Bothra
authors.forename: Arnab
authors.initials: A
authors.lastname: Sen
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000235:genetics; D019143:Evolution, Molecular; D040161:Frankia / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D020440:Gene Duplication; D008958:Models, Molecular; D008983:Molybdoferredoxin / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D005810:Multigene Family; D009586:Nitrogen Fixation / Q000235:genetics; D010088:Oxidoreductases / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D012641:Selection, Genetic; D040681:Structural Homology, Protein
chemical_list: D000596:Amino Acids; D008983:Molybdoferredoxin; D010088:Oxidoreductases; C029130:nitrogenase reductase; D044925:Oxidoreductases Acting on CH-CH Group Donors; C023886:protochlorophyllide reductase