title: The role of adipose-derived stromal cells and hydroxypropylmethylcellulose in engineering cartilage tissue in vivo.
abstract: This study demonstrated a newly developed method using adipose tissue-derived stromal cells (ADSCs) and hydroxypropylmethylcellulose (HPMC) in building injectable tissue engineered cartilage in vivo. ADSCs from rabbit subcutaneous fatty tissue were cultured in chondrogenic differentiation medium and supplemented with transforming growth factor-β1 (TGF-β1) and basic fibroblast growth factor (bFGF). Histological, immunohistochemistry and RT-PCR analysis confirmed that the ADSCs differentiated into chondrocytes following induction. Induced ADSCs mixed with 15 % HPMC were injected into the subcutaneous tissue of nude mice and, after a period of 8 weeks, newly formed cartilage was observed at the site of injection. The ability of ADSCs cultured in the induction medium with TGF-β1 and bFGF to differentiate into chondrocytes and construct new cartilage indicates that ADSCs are suitable for use as seed cells in cartilage tissue engineering. HPMC, according to its good water solubility and being able to transform from liquid to solid at body temperature, was found to be an ideal scaffold for tissue engineering.
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xijing Hospital, Fourth Military Medical University, No.17 ChangLe West Street, Xi'an, 710032, ShaanXi, People's Republic of China.
authors.forename: YuQiao
authors.initials: Y
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Ma
authors.forename: Yu
authors.initials: Y
authors.lastname: Han
authors.forename: Jie
authors.initials: J
authors.lastname: Min
authors.forename: YuanYuan
authors.initials: Y
authors.lastname: Liang
authors.forename: DaQing
authors.initials: D
authors.lastname: Zhao
authors.forename: JianHua
authors.initials: J
authors.lastname: Qiu