title: Comparison of the short term toxicity of phthalate diesters and monoesters in sprague-dawley male rats.
abstract: This study was carried out to investigate the short term toxicity of nine phthalate diesters including di-2 (ethylhexyl) phthalate (DEHP) , di (n-butyl) phthalate (DBP) , di-n-octyl phthalate (DnOP) , diethyl phthalate (DEP) , butylbenzyl phthalate (BBP) , dimethyl phthalate (DMP) , di-isodecyl phthalate (DIDP) , diundecyl phthalate (DUP) , and di-isononyl phthalate (DINP) and five phthalate monoesters including mono- (2-ethylhexyl) phthalate (MEHP) , monobutyl phthalate (MBuP) , monobenzyl phthalate (MBeP) , monoethyl phthalate (MEP) , monomethyl phthalate (MMP) and phthalic acid (PA) in Sprague-Dawley male rats. Animals were administered 250 mg/kg/day (monoesters and PA) or 500 mg/kg/day (diesters) of phthalate for two weeks. All animals were examined for body and organ weights, blood hematology, serum biochemistry, and urine analysis. The body weight gain was significantly lower in rats treated with BBP, DBP, DINP, MEHP, MBuP, and PA than that of control. Liver weights were significantly increased in the DEHP,DBP, DnOP, DIDP, and MEHP groups as compared to the control group. Testes weights were significantly decreased only in the DEHP-, DnOP-, and DIDP-treated groups as compared to the control. Significant differences in hematological changes were not observed in any treatment groups. Significant increases in blood glucose levels were observed in the DEHP, MEHP, and MBeP groups. Aspartate aminotransferase (AST) levels were significantly increased in the DBP, DUP, DINP, MBuP, and MBeP groups, whereas alanine aminotransferase (ALT) levels were significantly increased only in the DEHP and MEHP groups. Serum ALP levels were significantly higher in phthalate diester (500 mg/kg/day) -treated rats as compared to control. However, the total cholesterol level was significantly reduced in the DEHP- and DIDP-treated groups, whereas serum triglyceride (TG) levels were higher in the DINP-, MEHP-, and MBuP-treated groups. These results suggest that short term toxicity of phthalate monoesters produces adverse effects as similar to phthalate diesters in Sprague-Dawley rats.
authors.affiliation: National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration, Seoul 122-704.
authors.forename: Seung Jun
authors.initials: SJ
authors.lastname: Kwack
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Han
authors.forename: Jae Seok
authors.initials: JS
authors.lastname: Park
authors.forename: Jung Yun
authors.initials: JY
authors.lastname: Bae
authors.forename: Il Young
authors.initials: IY
authors.lastname: Ahn
authors.forename: Seong Kwang
authors.initials: SK
authors.lastname: Lim
authors.forename: Dong Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Dong Eun
authors.initials: DE
authors.lastname: Jang
authors.forename: Lan
authors.initials: L
authors.lastname: Choi
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Lim
authors.forename: Tae Hyung
authors.initials: TH
authors.lastname: Kim
authors.forename: Nabanita
authors.initials: N
authors.lastname: Patra
authors.forename: Kui Lea
authors.initials: KL
authors.lastname: Park
authors.forename: Hyung Sik
authors.initials: HS
authors.lastname: Kim
authors.forename: Byung Mu
authors.initials: BM
authors.lastname: Lee
keywords: Hematological changes; Phthalate diesters; Phthalate monoesters; Phthalic acid; Toxicity