title: A novel cell adhesion region in tropoelastin mediates attachment to integrin αVβ5.
abstract: Tropoelastin protein monomers assemble to form elastin. Cellular integrin αVβ3 binds RKRK at the C-terminal tail of tropoelastin. We probed cell interactions with tropoelastin by deleting the RKRK sequence to identify other cell-binding interactions within tropoelastin. We found a novel human dermal fibroblast attachment and spreading site on tropoelastin that is located centrally in the molecule. Inhibition studies demonstrated that this cell adhesion was not mediated by either elastin-binding protein or glycosaminoglycans. Cell interactions were divalent cation-dependent, indicating integrin dependence. Function-blocking monoclonal antibodies revealed that αV integrin(s) and integrin αVβ5 specifically were critical for cell adhesion to this part of tropoelastin. These data reveal a common αV integrin-binding theme for tropoelastin: αVβ3 at the C terminus and αVβ5 at the central region of tropoelastin. Each αV region contributes to fibroblast attachment and spreading, but they differ in their effects on cytoskeletal assembly.
authors.affiliation: From the School of Molecular Bioscience.
authors.forename: Pearl
authors.initials: P
authors.lastname: Lee
authors.forename: Daniel V
authors.initials: DV
authors.lastname: Bax
authors.forename: Marcela M M
authors.initials: MM
authors.lastname: Bilek
authors.forename: Anthony S
authors.initials: AS
authors.lastname: Weiss
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary; D019030:Receptors, Vitronectin / Q000235:genetics / Q000378:metabolism*; D014333:Tropoelastin / Q000235:genetics / Q000378:metabolism*
chemical_list: D039302:Integrin alphaVbeta3; D019030:Receptors, Vitronectin; D014333:Tropoelastin; C064660:integrin alphaVbeta5
keywords: Cell Adhesion; Dermal Fibroblast; Elastin; Extracellular Matrix; Fibroblast; Integrin; Multiple Integrins; Tropoelastin