title: Anti-inflammatory Activity of Dichloromethane Extract of Auricularia auricula-judae in RAW264.7 Cells.
abstract: The present study investigated the anti-inflammatory effects of dichloromethane extract of Auricularia auricula-judae. Dichloromethane extract of Auricularia auricula-judae inhibited Lipopolysaccharide (LPS) -induced nitric oxide (NO) production significantly in a dose-dependent manner in the concentration ≥10 μg/ml (p < 0.05) . Furthermore, RT-PCR results of this study indicated that the extract markedly reduced the expressions of inflammatory cytokines (IL-6, TNF-α and IL-1β) mRNA in LPS-treated murine RAW 264.7 macrophages, which could possibly ameliorate the inflammation. Nevertheless, dichloromethane extract of Auricularia auricula-judae did not show complete inhibition of IL-6 mRNA expression. The inhibition of IL-1β cytokine at protein level was also observed in a dose dependent manner. In conclusion,the current study revealed the previously unknown effect of dichloromethane ethyl extract of Auricularia auricula-judae inhibitions of the production of NO, IL-6, TNF-α and IL-1β in LPS-stimulated macrophages.
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu 702-701.
authors.forename: Dereje
authors.initials: D
authors.lastname: Damte
authors.forename: Md Ahsanur
authors.initials: MA
authors.lastname: Reza
authors.forename: Seung-Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Woo-Sik
authors.initials: WS
authors.lastname: Jo
authors.forename: Seung-Chun
authors.initials: SC
authors.lastname: Park
keywords: Anti-inflammatory; Auricularia auricula-judae; Inflammatory cytokines; Nitric oxide