title: Stability of human papillomavirus (HPV) in cervical ThinPrep specimens previously lysed with glacial acetic acid: effect on cobas 4800 HPV test performance.
abstract: BACKGROUND
  The authors previously demonstrated that lysing samples with glacial acetic acid (GAA) before human papillomavirus (HPV) testing does not adversely affect HPV detection with the cobas 4800 HPV Test. However, the long-term impact of GAA on HPV DNA was not explored in that study, and inherent cell loss with the lysing protocol used also was observed. The current study considered the long-term effects of GAA treatment of cervical ThinPrep samples on HPV detection with the cobas 4800 HPV Test. They also modified the manufacturer's lysing procedure for ThinPrep specimens to help prevent cell loss.
  
  
  METHODS
  Seventy-eight ThinPrep samples, including previously lysed, archived specimens, were split; then, 1 part was treated with GAA according to the manufacturer's protocol or using a modified protocol, and the other part was left untreated. All samples were tested for HPV using the cobas 4800 HPV Test. The HPV-positive/HPV-negative status of tested samples was used to determine the level of agreement between treated and untreated fractions. Performance of the modified lysing procedure was assessed relative to the manufacturer's protocol.
  
  
  RESULTS
  Positive HPV status produced 97% agreement between the GAA-treated/untreated fractions. Concordance between the GAA-treated/untreated fractions did not differ between the 2 lysing methods; however, the average percentage increases in β-globin and HPV cycle threshold values after lysing were less discernible for the modified lysing method.
  
  
  CONCLUSIONS
  GAA treatment of cervical ThinPrep specimens does not have long-term adverse effects on HPV detection with the cobas 4800 HPV Test. The manufacturer's GAA lysing procedure for ThinPrep specimens can be reliably modified for specimens that may subsequently undergo HPV testing.
authors.affiliation: Cytopathology Department, Altnagelvin Hospital, Western Health and Social Care Trust, Londonderry, Northern Ireland, United Kingdom.
authors.forename: Mary
authors.initials: M
authors.lastname: McMenamin
authors.forename: Michael
authors.initials: M
authors.lastname: McKenna
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D003581:Cytodiagnosis / Q000379:methods; D005260:Female; D006801:Humans; D008403:Mass Screening / Q000379:methods; D008867:Microtomy / Q000379:methods; D025202:Molecular Diagnostic Techniques; D027383:Papillomaviridae / Q000302:isolation & purification* / Q000472:pathogenicity; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000473:pathology / Q000821:virology*; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000473:pathology* / Q000821:virology*; D014626:Vaginal Smears / Q000379:methods; D019562:Viral Load; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis / Q000473:pathology* / Q000821:virology*
chemical_list: D019342:Acetic Acid
keywords: Hologic; ThinPrep; cobas 4800 HPV Test; glacial acetic acid; liquid-based cytology; unsatisfactory specimen