title: MiR-495 regulates proliferation and migration in NSCLC by targeting MTA3.
abstract: Our previous studies have showed that metastasis-associated protein 3 (MTA 3) is overexpressed in non-small cell lung cancer (NSCLC) tissue, and increased MTA3 mRNA levels is a risk factor of lymph node metastasis. Using bioinformatics analyses, we found that MTA3 was a potential target of miR-495. However, the pathophysiological role of miR-495 and its relevance to the growth and development of NSCLC have yet to be investigated. The purpose of this study was to elucidate the molecular mechanisms by which miR-495 acts as a tumor suppressor in NSCLC. qRT-PCR data showed significant downregulation of miR-495 in 56 NSCLC tissue samples and 5 lung cancer cell lines, compared with their adjacent normal tissue; furthermore, western blotting analysis revealed MTA3 protein was overexpressed in the tumor samples compared with the matched adjacent normal tissue. MiR-495 was shown to not only inhibit the proliferation of lung cancer cells (A549 and Calu-3) but also to inhibit cell migration in vitro. Using western blotting and luciferase assays, MTA3 was identified as a target of miR-495. These findings suggest the importance of miR-495 targeting of MTA3 in the regulation of lung cancer growth and migration.
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe Road, Zhengzhou, 450052, Henan, China.
authors.forename: Heying
authors.initials: H
authors.lastname: Chu
authors.forename: Xudong
authors.initials: X
authors.lastname: Chen
authors.forename: Huaqi
authors.initials: H
authors.lastname: Wang
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Du
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Wenqiao
authors.initials: W
authors.lastname: Zang
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Jingxia
authors.initials: J
authors.lastname: Chang
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhao
authors.forename: Guojun
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D016193:G1 Phase; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000502:physiology*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*
chemical_list: C577768:MIRN495 microRNA, human; C486657:MTA3 protein, human; D035683:MicroRNAs; D009363:Neoplasm Proteins