title: Highly tunable aptasensing microarrays with graphene oxide multilayers.
abstract: A highly tunable layer-by-layer (LbL)-assembled graphene oxide (GO) array has been devised for high-throughput multiplex protein sensing. In this array, the fluorescence of different target-bound aptamers labeled with dye is efficiently quenched by GO through fluorescence resonance energy transfer (FRET), and simultaneous multiplex target detection is performed by recovering the quenched fluorescence caused by specific binding between an aptamer and a protein. Thin GO films consisting of 10 bilayers displayed a high quenching ability, yielding over 85% fluorescence quenching with the addition of a 2 μM dye-labeled aptamer. The limit for human thrombin detection in the 6- and 10-bilayered GO array is estimated to be 0.1 and 0.001 nM, respectively, indicating highly tunable nature of LbL assembled GO multilayers in controlling the sensitivity of graphene-based FRET aptasensor. Furthermore, the GO chip could be reused up to four times simply by cleaning it with distilled water.
authors.affiliation: Interdisciplinary School of Green Energy, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Eonyang-eup, Ulju-gun, Ulsan 689-798, Republic of Korea.
authors.forename: Yun Kyung
authors.initials: YK
authors.lastname: Jung
authors.forename: Taemin
authors.initials: T
authors.lastname: Lee
authors.forename: Eeseul
authors.initials: E
authors.lastname: Shin
authors.forename: Byeong-Su
authors.initials: BS
authors.lastname: Kim
mesh_terms: D052157:Aptamers, Nucleotide / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005456:Fluorescent Dyes; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D036103:Nanotechnology / Q000379:methods; D010087:Oxides / Q000737:chemistry*; D013917:Thrombin / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D010087:Oxides; D006108:Graphite; D013917:Thrombin