title: Performance and microbial community of completely autotrophic nitrogen removal over nitrite (CANON) process in two membrane bioreactors (MBR) fed with different substrate levels.
abstract: To study the influence of substrate on completely autotrophic nitrogen removal over nitrite (CANON) process, two membrane bioreactors (MBR) with identical setup but fed with different substrate levels (R1 with low ammonia, R2 with high ammonia), were adopted in this study. The nitrogen removal performance, bioactivity, biodiversity and distribution of the functional microorganisms in two reactors were investigated. Both the aerobic ammonia-oxidizing bacteria (AerAOB) and anaerobic ammonia-oxidizing bacteria (AnAOB) in R2 showed higher bioactivity than those in R1, while nitrite-oxidizing bacteria (NOB) showed the contrary result. Nitrosomonas and Candidatus Kuenenia stuttgartiensis were detected as predominant functional microbes in the two reactors while Nitrobacter only existed in R1. High influent ammonia possibly led to the higher biodiversity of AerAOB and the more densely packed distribution. Meanwhile, this study has demonstrated the feasibility of increasing ammonia for rapid start-up, and decreasing HRT for high-rate nitrogen removal in CANON process.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China. Electronic address: lidong2006@bjut.edu.cn.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Huiping
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: He
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China. Electronic address: hitzhangjie@163.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000641:Ammonia / Q000378:metabolism; D000693:Anaerobiosis; D052818:Autotrophic Processes; D001419:Bacteria / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D058645:Denaturing Gradient Gel Electrophoresis; D052918:Environmental Restoration and Remediation / Q000295:instrumentation* / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D009573:Nitrites / Q000378:metabolism*; D009584:Nitrogen / Q000302:isolation & purification*; D010084:Oxidation-Reduction; D012722:Sewage / Q000382:microbiology; D014865:Waste Disposal, Fluid
chemical_list: D009573:Nitrites; D012722:Sewage; D000641:Ammonia; D009584:Nitrogen
keywords: AerAOB; AnAOB; CANON; DGGE; Nitrogen