title: Interaction of human minichromosome maintenance protein-binding protein with minichromosome maintenance 2-7.
abstract: It has been reported that minichromosome maintenance protein-binding protein (MCM-BP) functions in the formation of the pre-replication complex, unloading of minichromosome maintenance (MCM)2-7 from chromatin in late S phase, and formation of the cohesion complex by interacting with MCM3-7 proteins, suggesting that MCM-BP functions in several different reactions during the cell cycle. Here, we examined the interaction of human MCM-BP with MCM2-7 and structural maintenance of chromosome 3 in synchronized HeLa cells by immunoprecipitation. The results show that MCM-BP mainly interacts with MCM7 in the Triton-soluble fraction from S phase and G(2) phase cells, and it also interacts with structural maintenance of chromosome 3 in the fraction from G(2) phase cells. In vitro studies show that MCM-BP disassembles MCM2-7 bound to DNA with a fork-like structure by interacting with MCM3, MCM5, and MCM7. These results suggest that MCM-BP functions in disassembling MCM2-7 on chromatin during S phase and G2 phase by interacting with MCM3, MCM5, and MCM7.
authors.affiliation: College of Science, Ibaraki University, Mito, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Kusunoki
authors.forename: Yukio
authors.initials: Y
authors.lastname: Ishimi
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism; D002893:Chromosomes, Human, Pair 3 / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D064111:Minichromosome Maintenance Complex Component 2 / Q000378:metabolism; D064126:Minichromosome Maintenance Complex Component 3 / Q000378:metabolism; D064168:Minichromosome Maintenance Complex Component 7 / Q000378:metabolism; D064110:Minichromosome Maintenance Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; C066470:MCM3 protein, human; C489423:MCM5 protein, human; C519407:MCMBP protein, human; D009687:Nuclear Proteins; C084859:MCM2 protein, human; C101624:MCM7 protein, human; D064111:Minichromosome Maintenance Complex Component 2; D064126:Minichromosome Maintenance Complex Component 3; D064168:Minichromosome Maintenance Complex Component 7; D064110:Minichromosome Maintenance Proteins
keywords: DNA replication fork; minichromosome maintenance protein-binding protein; minichromosome maintenance 2-7; protein interaction; structural maintenance of chromosome