title: A dual gene-silencing vector system for monocot and dicot plants.
abstract: Plant virus-based gene-silencing vectors have been extensively and successfully used to elucidate functional genomics in plants. However, only limited virus-induced gene-silencing (VIGS) vectors can be used in both monocot and dicot plants. Here, we established a dual gene-silencing vector system based on Bamboo mosaic virus (BaMV) and its satellite RNA (satBaMV). Both BaMV and satBaMV vectors could effectively silence endogenous genes in Nicotiana benthamiana and Brachypodium distachyon. The satBaMV vector could also silence the green fluorescent protein (GFP) transgene in GFP transgenic N. benthamiana. GFP transgenic plants co-agro-inoculated with BaMV and satBaMV vectors carrying sulphur and GFP genes, respectively, could simultaneously silence both genes. Moreover, the silenced plants could still survive with the silencing of genes essential for plant development such as heat-shock protein 90 (Hsp90) and Hsp70. In addition, the satBaMV- but not BaMV-based vector could enhance gene-silencing efficiency in newly emerging leaves of N. benthamiana deficient in RNA-dependant RNA polymerase 6. The dual gene-silencing vector system of BaMV and satBaMV provides a novel tool for comparative functional studies in monocot and dicot plants.
authors.affiliation: Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan; Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
authors.forename: Ming-Ru
authors.initials: MR
authors.lastname: Liou
authors.forename: Ying-Wen
authors.initials: YW
authors.lastname: Huang
authors.forename: Chung-Chi
authors.initials: CC
authors.lastname: Hu
authors.forename: Na-Sheng
authors.initials: NS
authors.lastname: Lin
authors.forename: Yau-Heiu
authors.initials: YH
authors.lastname: Hsu
mesh_terms: D058431:Brachypodium / Q000235:genetics*; D020868:Gene Silencing; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified; D017863:Potexvirus / Q000235:genetics*; D019244:RNA, Satellite / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D036226:Seedlings / Q000235:genetics; D013045:Species Specificity; D014026:Nicotiana / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D019244:RNA, Satellite; D012367:RNA, Viral; D049452:Green Fluorescent Proteins; D012324:RNA-Dependent RNA Polymerase
keywords: Bamboo mosaic virus; RNA-dependant RNA polymerase 6; VIGS vector; satellite RNA; satellite RNA-based vector; virus-induced gene silencing