title: The influence of bioaugmentation and biosurfactant addition on bioremediation efficiency of diesel-oil contaminated soil: feasibility during field studies.
abstract: The study focused on assessing the influence of bioaugmentation and addition of rhamnolipids on diesel oil biodegradation efficiency during field studies. Initial laboratory studies (measurement of emitted CO2 and dehydrogenase activity) were carried out in order to select the consortium for bioaugmentation as well as to evaluate the most appropriate concentration of rhamnolipids. The selected consortium consisted of following bacterial taxa: Aeromonas hydrophila, Alcaligenes xylosoxidans, Gordonia sp., Pseudomonas fluorescens, Pseudomonas putida, Rhodococcus equi, Stenotrophomonas maltophilia, Xanthomonas sp. It was established that the application of rhamnolipids at 150 mg/kg of soil was most appropriate in terms of dehydrogenase activity. Based on the obtained results, four treatment methods were designed and tested during 365 days of field studies: I) natural attenuation; II) addition of rhamnolipids; III) bioaugmentation; IV) bioaugmentation and addition of rhamnolipids. It was observed that bioaugmentation contributed to the highest diesel oil biodegradation efficiency, whereas the addition of rhamnolipids did not notably influence the treatment process.
authors.affiliation: Institute of Chemical Technology and Engineering, Poznan University of Technology, Pl. M. Skłodowskiej-Curie 2, 60-965 Poznań, Poland. Electronic address: alicja_szulc@wp.pl.
authors.forename: Alicja
authors.initials: A
authors.lastname: Szulc
authors.affiliation: Institute of Chemical Technology and Engineering, Poznan University of Technology, Pl. M. Skłodowskiej-Curie 2, 60-965 Poznań, Poland.
authors.forename: Damian
authors.initials: D
authors.lastname: Ambrożewicz
authors.affiliation: Institute of Chemical Technology and Engineering, Poznan University of Technology, Pl. M. Skłodowskiej-Curie 2, 60-965 Poznań, Poland.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Sydow
authors.affiliation: Institute of Chemical Technology and Engineering, Poznan University of Technology, Pl. M. Skłodowskiej-Curie 2, 60-965 Poznań, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Ławniczak
authors.affiliation: Institute of Food Technology of Plant Origin, Poznań University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Piotrowska-Cyplik
authors.affiliation: Department of Biotechnology and Food Microbiology, University of Life Sciences in Poznań, Wojska Polskiego 48, 60-627 Poznań, Poland.
authors.forename: Roman
authors.initials: R
authors.lastname: Marecik
authors.affiliation: Institute of Chemical Technology and Engineering, Poznan University of Technology, Pl. M. Skłodowskiej-Curie 2, 60-965 Poznań, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Chrzanowski
mesh_terms: D001673:Biodegradation, Environmental; D052918:Environmental Restoration and Remediation / Q000379:methods*; D005742:Gasoline / Q000032:analysis*; D006017:Glycolipids / Q000378:metabolism*; D011044:Poland; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D005742:Gasoline; D006017:Glycolipids; D012987:Soil; D012989:Soil Pollutants; C418382:rhamnolipid
keywords: Bioaugmentation; Biodegradation; Bioremediation; Diesel oil; Rhamnolipids