title: Aptamer-conjugated gold nanorod for photothermal ablation of epidermal growth factor receptor-overexpressed epithelial cancer.
abstract: Biomarker-specific photothermal nanoparticles that can efficiently sense markers that are overexpressed in distinguished adenocarcinomas have attracted much interest in an aspect of efficacy increase of cancer treatment. We demonstrated a promising prospect of a smart photothermal therapy agent employing anti-epidermal growth factor receptor aptamer (AptEGFR)-conjugated polyethylene glycol (PEG) layted gold nanorods (AptEGFR-PGNRs). The cetyltrimethylammonium bromide bilayer on GNRs was replaced with heterobifunctional PEG (COOH-PEG-SH) not only to serve as a biocompatible stabilizer and but also to conjugate AptEGFR. Subsequently, to direct photothermal therapy agent toward epithelial cancer cells, the carboxylated PEGylated GNRs (PGNRs) were further functionalized with AptEGFR using carbodiimide chemistry. Then, to assess the potential as biomarker-specific photothermal therapy agent of synthesized AptEGFR-PGNRs, the optical properties, biocompatibility, colloidal stability, binding affinity, and epicellial cancer cell killing efficacy in vitro/in vivo under near-infrared laser irradiation were investigated. As a result, AptEGFR-PGNRs exhibit excellent tumor targeting ability and feasibility of effective photothermal ablation cancer therapy.
authors.affiliation: Yonsei University, Department of Chemical and Biomolecular Engineering, Seoul 120-749, Republic of Korea.
authors.forename: Jihye
authors.initials: J
authors.lastname: Choi
authors.forename: Yeonji
authors.initials: Y
authors.lastname: Park
authors.forename: Eun Bi
authors.initials: EB
authors.lastname: Choi
authors.forename: Hyun-Ouk
authors.initials: HO
authors.lastname: Kim
authors.forename: Dong Joo
authors.initials: DJ
authors.lastname: Kim
authors.forename: Yoochan
authors.initials: Y
authors.lastname: Hong
authors.forename: Sung-Ho
authors.initials: SH
authors.lastname: Ryu
authors.forename: Jung Hwan
authors.initials: JH
authors.lastname: Lee
authors.forename: Jin-Suck
authors.initials: JS
authors.lastname: Suh
authors.forename: Jaemoon
authors.initials: J
authors.lastname: Yang
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Huh
authors.forename: Seungjoo
authors.initials: S
authors.lastname: Haam
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D066246:ErbB Receptors / Q000235:genetics* / Q000378:metabolism; D006046:Gold / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D043942:Nanotubes / Q000737:chemistry*; D009375:Neoplasms, Glandular and Epithelial / Q000378:metabolism; D010789:Phototherapy / Q000379:methods*; D023041:Xenograft Model Antitumor Assays
chemical_list: D052157:Aptamers, Nucleotide; D006046:Gold; D066246:ErbB Receptors