title: Enhanced dechlorination of carbon tetrachloride by Geobacter sulfurreducens in the presence of naturally occurring quinones and ferrihydrite.
abstract: The effect of naturally occurring quinones including lawsone (LQ), ubiquinone (UQ), juglone (JQ), and 1,4-naphthoquinone (NQ) on the biotransformation of carbon tetrachloride (CT) in the presence of Geobacter sulfurreducens and ferrihydrite was investigated. AQDS was used as the model compound for comparison. The reductive dissolution of ferrihydrite by G. sulfurreducens was enhanced by AQDS, NQ, and LQ. However, addition of UQ and JQ had little enhancement effect on Fe(II) production. The bioreduction efficiency and rate of ferrihydrite was highly dependent on the natural property and concentration of quinone compounds and the addition of low concentrations of LQ and NQ significantly accelerated the biotransformation rate of CT. The pseudo-first-order rate constants for CT dechlorination (kobsCT) in AQDS-, LQ- and NQ-amended batches were 5.4-5.8, 4.6-7.4 and 2.4-5.8 times, respectively, higher than those in the absence of quinone. A good relationship between kobsCT for CT dechlorination and bioreduction ratio of ferrihydrite was observed, indicating the important role of biogenic Fe(II) in dechlorination of CT under iron-reducing conditions. Spectroscopic analysis showed that AQDS and NQ could be reduced to semiquinones and hydroquinones, while only hydroquinones were generated in LQ-amended batches.
authors.affiliation: Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, 101, Sec. 2, Kuang Fu Road, Hsinchu 30013, Taiwan. Electronic address: radoong@mx.nthu.edu.tw.
authors.forename: Ruey-an
authors.initials: RA
authors.lastname: Doong
authors.affiliation: Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, 101, Sec. 2, Kuang Fu Road, Hsinchu 30013, Taiwan.
authors.forename: Chun-chi
authors.initials: CC
authors.lastname: Lee
authors.affiliation: Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, 101, Sec. 2, Kuang Fu Road, Hsinchu 30013, Taiwan.
authors.forename: Chia-min
authors.initials: CM
authors.lastname: Lien
mesh_terms: D001673:Biodegradation, Environmental; D002251:Carbon Tetrachloride / Q000032:analysis / Q000378:metabolism*; D005290:Ferric Compounds; D005296:Ferrous Compounds / Q000378:metabolism; D045302:Geobacter / Q000378:metabolism*; D015386:Hazardous Substances / Q000378:metabolism; D009285:Naphthoquinones / Q000378:metabolism; D010084:Oxidation-Reduction; D011809:Quinones / Q000378:metabolism; D012997:Solvents / Q000378:metabolism
chemical_list: D005290:Ferric Compounds; D005296:Ferrous Compounds; D015386:Hazardous Substances; D009285:Naphthoquinones; D011809:Quinones; D012997:Solvents; C092844:ferric oxyhydroxide; D002251:Carbon Tetrachloride; C005090:lawsone; C005134:juglone
keywords: Biogenic Fe(II); Carbon tetrachloride; Ferrihydrite; Geobacter sulfurreducens; Naturally occurring quinones