title: Benzoboroxole-functionalized magnetic core/shell microspheres for highly specific enrichment of glycoproteins under physiological conditions.
abstract: Efficient enrichment of specific glycoproteins from complex biological samples is of great importance towards the discovery of disease biomarkers in biological systems. Recently, phenylboronic acid-based functional materials have been widely used for enrichment of glycoproteins. However, such enrichment was mainly carried out under alkaline conditions, which is different to the status of glycoproteins in neutral physiological conditions and may cause some unpredictable degradation. In this study, on-demand neutral enrichment of glycoproteins from crude biological samples is accomplished by utilizing the reversible interaction between the cis-diols of glycoproteins and benzoboroxole-functionalized magnetic composite microspheres (Fe3O4/PAA-AOPB). The Fe3O4/PAA-AOPB composite microspheres are deliberately designed and constructed with a high-magnetic-response magnetic supraparticle (MSP) core and a crosslinked poly(acrylic acid) (PAA) shell anchoring abundant benzoboroxole functional groups on the surface. These nanocomposites possessed many merits, such as large enrichment capacity (93.9 mg/g, protein/beads), low non-specific adsorption, quick enrichment process (10 min) and magnetic separation speed (20 s), and high recovery efficiency. Furthermore, the as-prepared Fe3O4/PAA-AOPB microspheres display high selectivity to glycoproteins even in the E. coli lysate or fetal bovine serum, showing great potential in the identify of low-abundance glycoproteins as biomarkers in real complex biological systems for clinical diagnoses.
authors.affiliation: State Key Laboratory of Molecular, Engineering of Polymers, Department of Macromolecular Science and Laboratory of Advanced Materials, Fudan University, Shanghai, 200433, People's Republic of China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wanfu
authors.initials: W
authors.lastname: Ma
authors.forename: Dian
authors.initials: D
authors.lastname: Li
authors.forename: Meng
authors.initials: M
authors.lastname: Yu
authors.forename: Jia
authors.initials: J
authors.lastname: Guo
authors.forename: Changchun
authors.initials: C
authors.lastname: Wang
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D001896:Boron Compounds / Q000737:chemistry*; D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006023:Glycoproteins / Q000302:isolation & purification*; D060328:Magnetic Phenomena; D008863:Microspheres; D058105:Polymerization; D014961:X-Ray Diffraction
chemical_list: D000180:Acrylic Resins; D001896:Boron Compounds; D006023:Glycoproteins; C006903:carbopol 940; D052203:Ferrosoferric Oxide
keywords: Fe3O4/PAA-AOPB core/shell microspheres; benzoboroxole; glycoproteins; physiological condition; selective enrichment