title: Microdissection and visualization of individual hair follicles for lineage tracing studies.
abstract: In vivo lineage tracing is a valuable technique to study cellular behavior. Our lab developed a lineage tracing method, based on the Cre/lox system, to genetically induce clonal labelling of cells and follow their progeny. Here we describe a protocol for temporally controlled clonal labelling and for microdissection of individual mouse hair follicles. We further present staining and visualization techniques used in our lab to analyze clones issued from genetically induced labelling.
authors.affiliation: Unité de Biologie moléculaire du Développement, Institut Pasteur, 25, rue du Docteur Roux, Paris Cedex 15, F-75724, France, inesequeira@gmail.com.
authors.forename: Inês
authors.initials: I
authors.lastname: Sequeira
authors.forename: Emilie
authors.initials: E
authors.lastname: Legué
authors.forename: Suzanne
authors.initials: S
authors.lastname: Capgras
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Nicolas
mesh_terms: D000818:Animals; D019070:Cell Lineage / Q000187:drug effects; D002999:Clone Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D003314:Corn Oil / Q000737:chemistry; D017930:Genes, Reporter / Q000235:genetics; D005990:Glycerol / Q000031:analogs & derivatives / Q000737:chemistry; D018859:Hair Follicle / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D007267:Injections; D019426:Integrases / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D042282:Microdissection / Q000379:methods*; D018613:Microscopy, Confocal; D057054:Molecular Imaging / Q000379:methods*; D013194:Staining and Labeling; D013629:Tamoxifen / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D016707:Tissue Fixation
chemical_list: D008164:Luminescent Proteins; D013629:Tamoxifen; C016601:afimoxifene; C000515:cremophor EL; D003314:Corn Oil; C045073:Cre recombinase; D019426:Integrases; D005990:Glycerol