title: Phenotypic impact of deregulated expression of class I histone deacetylases in urothelial cell carcinoma of the bladder.
abstract: Deregulated expression of histone deacetylases (HDACs) has been implicated in tumorigenesis. Herein, we investigated class I HDACs expression in bladder urothelial cell carcinoma (BUCC), its prognostic value and biological significance. Significantly increased transcript levels of all HDACs were found in BUCC compared to 20 normal mucosas, and these were higher in lower grade and stage tumors. Increased HDAC3 levels were associated with improved patient survival. SiRNA experiments showed decrease cell viability and motility, and increased apoptosis. We concluded that class I HDACs play an important role in bladder carcinogenesis through deregulation of proliferation, migration and apoptosis, constituting putative therapeutic targets.
authors.affiliation: Cancer Epigenetics Group, Research Center of the Portuguese Oncology Institute-Porto, Porto, Portugal.
authors.forename: Susana
authors.initials: S
authors.lastname: Junqueira-Neto
authors.affiliation: Cancer Epigenetics Group, Research Center of the Portuguese Oncology Institute-Porto, Porto, Portugal.|School of Allied Health Sciences ESTSP, Polytechnic of Porto, Porto, Portugal.
authors.forename: Filipa Q
authors.initials: FQ
authors.lastname: Vieira
authors.affiliation: Cancer Epigenetics Group, Research Center of the Portuguese Oncology Institute-Porto, Porto, Portugal.|Department of Pathology, Portuguese Oncology Institute, Porto, Portugal.
authors.forename: Diana
authors.initials: D
authors.lastname: Montezuma
authors.affiliation: Cancer Epigenetics Group, Research Center of the Portuguese Oncology Institute-Porto, Porto, Portugal.
authors.forename: Natália R
authors.initials: NR
authors.lastname: Costa
authors.affiliation: Department of Epidemiology, Portuguese Oncology Institute, Porto, Portugal.
authors.forename: Luís
authors.initials: L
authors.lastname: Antunes
authors.affiliation: Cancer Epigenetics Group, Research Center of the Portuguese Oncology Institute-Porto, Porto, Portugal.
authors.forename: Tiago
authors.initials: T
authors.lastname: Baptista
authors.affiliation: Cancer Epigenetics Group, Research Center of the Portuguese Oncology Institute-Porto, Porto, Portugal.
authors.forename: Ana Isabel
authors.initials: AI
authors.lastname: Oliveira
authors.affiliation: Cancer Epigenetics Group, Research Center of the Portuguese Oncology Institute-Porto, Porto, Portugal.|School of Allied Health Sciences ESTSP, Polytechnic of Porto, Porto, Portugal.
authors.forename: Inês
authors.initials: I
authors.lastname: Graça
authors.affiliation: Department of Pathology, Portuguese Oncology Institute, Porto, Portugal.
authors.forename: Ângelo
authors.initials: Â
authors.lastname: Rodrigues
authors.affiliation: Department of Urology, Portuguese Oncology Institute, Porto, Portugal.
authors.forename: José S
authors.initials: JS
authors.lastname: Magalhães
authors.affiliation: Department of Urology, Portuguese Oncology Institute, Porto, Portugal.
authors.forename: Jorge
authors.initials: J
authors.lastname: Oliveira
authors.affiliation: Cancer Epigenetics Group, Research Center of the Portuguese Oncology Institute-Porto, Porto, Portugal.|Department of Pathology, Portuguese Oncology Institute, Porto, Portugal.|Department of Pathology and Molecular Immunology, Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, Portugal.
authors.forename: Rui
authors.initials: R
authors.lastname: Henrique
authors.affiliation: Cancer Epigenetics Group, Research Center of the Portuguese Oncology Institute-Porto, Porto, Portugal.|Department of Pathology and Molecular Immunology, Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, Portugal.
authors.forename: Carmen
authors.initials: C
authors.lastname: Jerónimo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006655:Histone Deacetylases / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D001743:Urinary Bladder / Q000378:metabolism / Q000473:pathology*; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology*
chemical_list: D034741:RNA, Small Interfering; D006655:Histone Deacetylases
keywords: apoptosis; bladder cancer; class I HDACs; viability