title: Targeting tissular immune response improves diagnostic performance of anti-Saccharomyces cerevisiae antibodies (ASCA) in Crohn's disease.
abstract: Antibodies against Saccharomyces cerevisiae (ASCA) and Escherichia coli outer membrane porin C (anti-OmpC) are known to be detectable in the serum of patients with Crohn's disease (CD) but display a very poor sensitivity for the disease especially in forms with isolated colonic involvement. In this study we aimed at evaluating performances of these markers in supernatant of cultured colonic biopsies. Patients with colonic CD (n =  67), ulcerative colitis (UC) (n = 35) and control individuals (n = 37) were prospectively recruited for colonoscopy pinch biopsies and blood sampling. Serum and supernatant of culture tissues were analyzed for ASCA and anti-OmpC. Direct immunofluorescence was also performed on colonic tissues for total IgA detection. We detected for the first time ASCA IgA/IgG and anti-OmpC IgA in cultured colonic tissue supernatants. For both markers, sensitivities for diagnosing CD were better in supernatants (ASCA: 53.7%, anti-OmpC: 28.4%) than in serum (ASCA: 31.3%, anti-OmpC: 22.4%). Combination of results from a panel of these tests gave the greatest sensitivity ever described for CD diagnosis in colonic forms (70.2%). In this study, we described, for the first time, ASCA in supernatant of colonic tissue cultures. This assaying approach in CD diagnosis should be taken into consideration in the future especially in CD forms with isolated colonic involvement.
authors.affiliation: Service d'Immunologie, Pôle de Biologie, Hôpital de la Conception, Assistance Publique-Hôpitaux de Marseille, Marseille, France ; Aix-Marseille Université, Centre national de la recherche scientifique, Neurobiologie des Interactions Cellulaires et Neurophysiopathologie UMR 7259, Marseille, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Bertin
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Grimaud
authors.forename: Nathalie
authors.initials: N
authors.lastname: Lesavre
authors.forename: Chahine
authors.initials: C
authors.lastname: Benelmouloud
authors.forename: Ariadne
authors.initials: A
authors.lastname: Desjeux
authors.forename: Stéphane
authors.initials: S
authors.lastname: Garcia
authors.forename: Sophie
authors.initials: S
authors.lastname: Desplat-Jégo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000908:Antibodies, Fungal / Q000097:blood / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D003093:Colitis, Ulcerative / Q000276:immunology / Q000382:microbiology; D003424:Crohn Disease / Q000175:diagnosis / Q000276:immunology* / Q000382:microbiology*; D017077:Culture Media, Conditioned; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008875:Middle Aged; D018272:Porins / Q000276:immunology; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000276:immunology*; D012680:Sensitivity and Specificity; D046509:Tissue Culture Techniques; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D000908:Antibodies, Fungal; D015415:Biomarkers; D017077:Culture Media, Conditioned; D007070:Immunoglobulin A; D007074:Immunoglobulin G; C113724:OmpC protein; D018272:Porins