title: A novel combination of nano-scaffolds with micro-scaffolds to mimic extracellularmatrices improve osteogenesis.
abstract: To improve bone engineering for clinical applications, we coupled nanofiber-peptide hydrogel to nano-hydroxyapatite/collagen to form a bioactive scaffold (cnHAC) that mimics extracellular matrices. In comparison to nano-hydroxyapatite/collagen, we found that cnHAC promoted cell adhesion and spreading, and DNA content measurements, alkaline phosphatase activity assays, and reverse transcriptase-polymerase chain reaction analyses of osteogenic gene expression showed that cnHAC significantly improved cellular attachment, proliferation, and osteogenic differentiation in vitro (P < 0.05). In vivo models based on rat calvarial implants showed that cnHAC significantly enhanced bone regeneration (P < 0.05). In conclusion, we demonstrated that novel cnHAC scaffolds could potentially facilitate future bone regenerative medicine.
authors.affiliation: Institute of Stomatology, Nanjing Medical University, Nanjing, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Institute of Stomatology, Nanjing Medical University, Nanjing, PR China Department of Stomatology, Yancheng No.1 People's Hospital, Yancheng, PR China.
authors.forename: Sha-Sha
authors.initials: SS
authors.lastname: Peng
authors.affiliation: Institute of Stomatology, Nanjing Medical University, Nanjing, PR China.
authors.forename: Li-Zhe
authors.initials: LZ
authors.lastname: Xie
authors.affiliation: Biomaterials Laboratory, Department of Materials Science and Engineering, Tsinghua University, Beijing, PR China School of Mechanics, Taiyuan University of Technology, Taiyuan, PR China.
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Lian
authors.affiliation: Beijing Allgens Medical Science & Technology Co., Ltd, Beijing, PR China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Zhang
authors.affiliation: Beijing Allgens Medical Science & Technology Co., Ltd, Beijing, PR China.
authors.forename: Han
authors.initials: H
authors.lastname: Cui
authors.affiliation: Beijing Allgens Medical Science & Technology Co., Ltd, Beijing, PR China.
authors.forename: Tian-Xi
authors.initials: TX
authors.lastname: Song
authors.affiliation: Institute of Stomatology, Nanjing Medical University, Nanjing, PR China fmzhang@njmu.edu.cn.
authors.forename: Fei-Min
authors.initials: FM
authors.lastname: Zhang
authors.affiliation: Suzhou Institute, Southeast University, Suzhou, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Gu
authors.affiliation: Biomaterials Laboratory, Department of Materials Science and Engineering, Tsinghua University, Beijing, PR China.
authors.forename: Fu-Zhai
authors.initials: FZ
authors.lastname: Cui
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen; D017886:Durapatite; D005109:Extracellular Matrix / Q000737:chemistry; D015870:Gene Expression; D006801:Humans; D020100:Hydrogels; D066298:In Vitro Techniques; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D010012:Osteogenesis / Q000235:genetics; D051381:Rats; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D018786:Bone Substitutes; D020100:Hydrogels; C509317:nano-hydroxyapatite-collagen; D003094:Collagen; D017886:Durapatite; D000469:Alkaline Phosphatase
keywords: Peptide hydrogel; adipose-derived stem cells; biocompatibility; bone regeneration; nano-hydroxyapatite/collagen