title: [Isolation and degradation characteristics of dichloromethane-degradation bacterial strain by Methylobacterium rhodesianum H13].
abstract: A dichloromethane-degrading bacterium Methylobacterium rhodesianum H13 which utilized the DCM as the sole carbon and energy source was isolated. According to the research, M. rhodesianum H13 could completely degrade 5 mmol x L(-1) DCM in 23 h with the initial cell concentration of 0.82 mg x L(-1), pH 7.0, 30 degrees C, and the cell yield rate was about 0.136 g x g(-1) DCM. With the degradation of DCM, Cl- concentration gradually raised (the release of Cl- concentration was about 2 times higher as the DCM), pH value dropped to 6.75, and the solution was weakly acidic. Temperature, pH, DCM concentration, Cl- concentration and other factors were investigated through the shake flask experiments, and the optimal conditions for DCM degradation were: temperature 30 degrees C, pH 7.0. The study also indicated that 5 mmol x L(-1) of DCM was the optimum concentration for M. rhodesianum H13 and high levels of DCM could inhibit the degradation. The research has an important application value for the DCM environmental pollution.
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China. liuhongxia2266@126.com
authors.forename: Hong-xia
authors.initials: HX
authors.lastname: Liu
authors.forename: Run-ye
authors.initials: RY
authors.lastname: Zhu
authors.forename: Du-juan
authors.initials: DJ
authors.lastname: Ouyang
authors.forename: Qing-feng
authors.initials: QF
authors.lastname: Zhuang
authors.forename: Dong-zhi
authors.initials: DZ
authors.lastname: Chen
authors.forename: Jian-meng
authors.initials: JM
authors.lastname: Chen
mesh_terms: D001673:Biodegradation, Environmental; D008752:Methylene Chloride / Q000378:metabolism*; D020580:Methylobacterium / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D008752:Methylene Chloride