title: Effect of yellow capsicum extract on proliferation and differentiation of 3T3-L1 preadipocytes.
abstract: OBJECTIVE
  To evaluate the effect of effect of Yellow Capsicum extract (YCE) that is rich in capsaicin on the proliferation and differentiation of 3T3-L1 preadipocytes in vitro.
  
  
  METHODS
  3T3 L1 cells that were exposed to differentiation-inducing medium containing high glucose DMEM (Dulbecco's Modified Eagle's Medium) and subsequently were treated with capsaicin and YCE for their effect on adipocyte differentiation, changes in their triglyceride content, leptin secretion, expression of lipoprotein lipase, PPARγ, and CCAAT/enhancer-binding protein alpha (C/EBPα).
  
  
  RESULTS
  Both YCE and capsaicin inhibited proliferation and differentiation 3T3-L1 preadipocytes and suppressed accumulation of intracellular triglyceride in a dose-dependent manner. In addition, a significant decrease in the expression of lipoprotein lipase (LPL), leptin, PPARγ, and C/EBPα was noted in 3T3-L1 preadipocytes when induced to differentiate by YCE and Capsaicin.
  
  
  CONCLUSIONS
  The potent inhibitory action of YCE and Capsaicin on the differentiation of 3T3-L1 preadipocyte observed suggests that they (YCE and Capsaicin) have the potential to inhibit obesity that needs to be explored in future studies.
authors.affiliation: Department of Stomatology, Children's Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Feng
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Hai-ning
authors.affiliation: Department of Food Science & Nutrition, College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Xiao-man
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Zun-chen
authors.affiliation: Department of Food Science & Nutrition, College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou, China. Electronic address: shrshen@zju.edu.cn.
authors.forename: Shen
authors.initials: S
authors.lastname: Sheng-rong
authors.affiliation: UND Life Sciences, Shaker Heights, Ohio, USA; Jawaharlal Nehru Technological University, Kakinada, India; Bio-Science Research Centre, Gayatri Vidya Parishad College of Engineering, Visakhapatnam, India. Electronic address: undurti@hotmail.com.
authors.forename: Undurti N
authors.initials: UN
authors.lastname: Das
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics / Q000378:metabolism; D002211:Capsaicin / Q000494:pharmacology; D002212:Capsicum / Q000737:chemistry*; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D020738:Leptin / Q000378:metabolism; D008071:Lipoprotein Lipase / Q000235:genetics / Q000378:metabolism; D051379:Mice; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D020738:Leptin; D047495:PPAR gamma; D010936:Plant Extracts; D014280:Triglycerides; D008071:Lipoprotein Lipase; D002211:Capsaicin
keywords: 3T3-L1; Differentiation; Obesity; Proliferation; Yellow capsicum extract