title: In vitro screening of compounds against laboratory and field isolates of human hookworm reveals quantitative differences in anthelmintic susceptibility.
abstract: A panel of 80 compounds was screened for anthelmintic activity against a laboratory strain of Ancylostoma ceylanicum and field isolates of hookworm obtained from school children in the Kintampo North District of the Brong Ahafo Region of Ghana. Although the laboratory strain of A. ceylanicum was more susceptible to the compounds tested than the field isolates of hookworm, a twofold increase in compound concentration resulted in comparable egg hatch percent inhibition for select compounds. These data provide evidence that the efficacy of anthelmintic compounds may be species-dependent and that field and laboratory strains of hookworm differ in their sensitivities to the anthelmintics tested. These data also suggest that both compound concentration and hookworm species must be considered when screening to identify novel anthelmintic compounds.
authors.affiliation: Department of Pediatrics and Program in International Child Health, Yale University School of Medicine, New Haven, Connecticut; Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana; Chemical Genomics Center, National Institutes of Health, Bethesda, Maryland; Yale School of Public Health, Yale University, New Haven, Connecticut.
authors.forename: Rebecca S
authors.initials: RS
authors.lastname: Treger
authors.forename: Joseph
authors.initials: J
authors.lastname: Otchere
authors.forename: Martin F
authors.initials: MF
authors.lastname: Keil
authors.forename: Josephine E
authors.initials: JE
authors.lastname: Quagraine
authors.forename: Ganesha
authors.initials: G
authors.lastname: Rai
authors.forename: Bryan T
authors.initials: BT
authors.lastname: Mott
authors.forename: Debbie L
authors.initials: DL
authors.lastname: Humphries
authors.forename: Michael
authors.initials: M
authors.lastname: Wilson
authors.forename: Michael
authors.initials: M
authors.lastname: Cappello
authors.forename: Jon J
authors.initials: JJ
authors.lastname: Vermeire
mesh_terms: D000722:Ancylostoma / Q000187:drug effects*; D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D004351:Drug Resistance; D005869:Ghana / Q000453:epidemiology; D006725:Hookworm Infections / Q000188:drug therapy* / Q000453:epidemiology; D006801:Humans; D010063:Ovum / Q000187:drug effects
chemical_list: D000871:Anthelmintics