title: Single layer linear array of microbeads for multiplexed analysis of DNA and proteins.
abstract: In this study, a microfluidic platform was developed to generate single layer, linear array of microbeads for multiplexed high-throughput analysis of biomolecules. The microfluidic device is comprised of eight microbead-trapping units, where microbeads were immobilized in a linear array format by the exertion of a negative pressure in the control channel connected to each sieving microstructure. Multiplexed assays were achieved by using a mixture of different spectrally-encoded microbeads functionalized with specific probes, followed by on-chip reaction and detection. The microfluidic-based microbeads array platform was employed for multiplexed analysis of DNA and proteins, as demonstrated by the simultaneous discrimination of four HPV genotypes and the parallel detection of six different proteins. Compared with the off-chip protocols, the on-chip analysis exhibited better reaction efficiency, higher sensitivity and wider linear detection range. Visual inspection and identification of functionalized microbeads were facilitated by the single layer arrangement of microbeads so that accurate data acquisition can be performed during the detection process.
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, People's Republic of China; Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institutes of City University of Hong Kong, Shenzhen, People's Republic of China.
authors.forename: Wanqing
authors.initials: W
authors.lastname: Yue
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, People's Republic of China; Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institutes of City University of Hong Kong, Shenzhen, People's Republic of China.
authors.forename: Heng
authors.initials: H
authors.lastname: Zou
authors.affiliation: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, People's Republic of China.
authors.forename: Qinghui
authors.initials: Q
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, People's Republic of China.
authors.forename: Cheuk-Wing
authors.initials: CW
authors.lastname: Li
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, People's Republic of China; Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institutes of City University of Hong Kong, Shenzhen, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, People's Republic of China; Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institutes of City University of Hong Kong, Shenzhen, People's Republic of China.
authors.forename: Huayang
authors.initials: H
authors.lastname: Fu
authors.affiliation: Multigene Diagnostics Limited, 5/F, Biotech Centre 2, 11 Science Park Avenue, Hong Kong Science Park, N. T., Hong Kong SAR, People's Republic of China.
authors.forename: Lawrence C H
authors.initials: LC
authors.lastname: Tzang
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, People's Republic of China; Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institutes of City University of Hong Kong, Shenzhen, People's Republic of China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, People's Republic of China.
authors.forename: Jianlong
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, People's Republic of China; Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institutes of City University of Hong Kong, Shenzhen, People's Republic of China. Electronic address: bhmyang@cityu.edu.hk.
authors.forename: Mengsu
authors.initials: M
authors.lastname: Yang
mesh_terms: D052685:Alphapapillomavirus / Q000235:genetics*; D015374:Biosensing Techniques / Q000295:instrumentation; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D004867:Equipment Design; D005838:Genotype; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008863:Microspheres; D030361:Papillomavirus Infections / Q000821:virology*; D011506:Proteins / Q000032:analysis*
chemical_list: D004279:DNA, Viral; D011506:Proteins
keywords: Microbead array; Microfluidics; Multiplexed assay