title: Airborne bacterial reduction by spraying slightly acidic electrolyzed water in a laying-hen house.
abstract: Spraying slightly acidic electrolyzed water (SAEW) has been considered as a potential approach to reduce airborne bacteria in laying-hen houses. In this study, the effects of spraying SAEW on airborne bacterial reduction were investigated in a laying-hen house as compared with using diluted didecyl dimethyl ammonium bromide (DDAB). Averaged air temperature reduced by approximate 1 degrees C and average relative humidity increased by 3% at a stable ventilation rate (about 2.5 m3 hr(-1) per bird) in the laying-hen house 30 min after spraying (120 mL m(-2)). Compared with the control without spraying, the airborne bacterial concentration was reduced by about 0.70 and 0.37 log10 colony-forming units (CFU) m(-3) in the 4 hr after spraying 120 mL m(-2) SAEW (available chlorine concentration [ACC] of 156 mg L(-1)) and diluted DDAB (active compound concentration of 167 mg L(-1)), respectively. Compared with spraying diluted DDAB, spraying SAEW was determined to be more effective for reducing airborne bacterial in laying-hen houses. The effects of spraying SAEW and diluted DDAB on airborne bacterial reduction in the laying-hen house increased with the increasing available chlorine concentrations for SAEW (156, 206, 262 mg L(-1)) and increasing active compound concentrations for diluted DDAB (167, 333, 500 mg L(-1)), respectively. Spraying SAEW and diluted DDAB with two levels of spraying volumes (120 and 90 mL m(-2)) both showed significant differences on airborne bacterial reduction in the laying-hen house (P < 0.05).
authors.affiliation: College of Water Conservancy and Civil Engineering, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Weichao
authors.initials: W
authors.lastname: Zheng
authors.forename: Runmin
authors.initials: R
authors.lastname: Kang
authors.forename: Hongning
authors.initials: H
authors.lastname: Wang
authors.forename: Baoming
authors.initials: B
authors.lastname: Li
authors.forename: Changwen
authors.initials: C
authors.lastname: Xu
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
mesh_terms: D000391:Air Microbiology / Q000592:standards*; D016902:Air Pollution, Indoor / Q000517:prevention & control*; D000818:Animals; D001419:Bacteria / Q000187:drug effects; D002645:Chickens; D004202:Disinfectants; D005260:Female; D006813:Humidity; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D010058:Oviposition; D013696:Temperature
chemical_list: D004202:Disinfectants; C118085:superoxidized water; D006861:Hydrogen Peroxide