title: High-throughput identification of chemical endomembrane cycling disruptors utilizing tobacco pollen.
abstract: Endomembrane cycling processes in plants remain mostly intractable through classical genetic interrogation. Chemical disruption of these processes provides an opportunity to slow or inhibit these processes for study. Tobacco pollen, which is dependent upon endomembrane cycling for tube growth, provides a plant system that is amenable to high-throughput screening of chemical disruptors. We describe here the process that allowed the identification of over 360 endomembrane cycling disruptors.
authors.affiliation: Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, CA, USA.
authors.forename: Michelle Q
authors.initials: MQ
authors.lastname: Brown
authors.forename: Nolan
authors.initials: N
authors.lastname: Ung
authors.forename: Natasha V
authors.initials: NV
authors.lastname: Raikhel
authors.forename: Glenn R
authors.initials: GR
authors.lastname: Hicks
mesh_terms: D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism*; D046508:Culture Techniques; D018525:Germination; D057166:High-Throughput Screening Assays; D011058:Pollen / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D054852:Small Molecule Libraries; D014026:Nicotiana / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
chemical_list: D054852:Small Molecule Libraries