title: Matrix metalloproteinase (MMP)-8 and tissue inhibitor of MMP-1 (TIMP-1) gene polymorphisms in generalized aggressive periodontitis: gingival crevicular fluid MMP-8 and TIMP-1 levels and outcome of periodontal therapy.
abstract: BACKGROUND
  The aim of the present study is to investigate matrix metalloproteinase (MMP)-8 and tissue inhibitor of MMP-1 (TIMP-1) gene polymorphisms in generalized aggressive periodontitis (GAgP) and to assess the effects of MMP-8 and TIMP-1 genotypes on the outcomes of non-surgical periodontal therapy.
  
  
  METHODS
  Genomic DNA was obtained from peripheral blood of 100 patients with GAgP and 167 periodontally healthy controls. MMP-8 +17 C/G, -799 C/T, -381 A/G and TIMP-1 372 T/C, *429 T/G polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism. Patients with GAgP received non-surgical periodontal therapy and were followed for 6 months. Clinical periodontal parameters and gingival crevicular fluid (GCF) samples were collected at baseline and at follow-up visits. GCF biomarkers were analyzed by immunofluorescence assay and enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Distribution of the MMP-8 -799 C/T genotypes was significantly different between the GAgP and control groups (P <0.005). TIMP-1 372 T/C and *429 T/G genotypes in males were also significantly different between study groups (P <0.004). GCF MMP-8 levels decreased until 3 months after non-surgical therapy compared with baseline in T and G alleles, as well as G and C allele carriers (P <0.0125), whereas no significant decreased was observed in non-carriers (P >0.0125).
  
  
  CONCLUSIONS
  On the basis of the present findings, it can be suggested that MMP-8 -799 C/T and TIMP-1 372 T/C, *429 T/G gene polymorphisms in males may be associated with the susceptibility to GAgP in the Turkish population.
authors.affiliation: Department of Periodontology, School of Dentistry, Ege University, İzmir, Turkey.
authors.forename: Gülnur
authors.initials: G
authors.lastname: Emingil
authors.forename: Buket
authors.initials: B
authors.lastname: Han
authors.forename: Ali
authors.initials: A
authors.lastname: Gürkan
authors.forename: Afig
authors.initials: A
authors.lastname: Berdeli
authors.forename: Taina
authors.initials: T
authors.lastname: Tervahartiala
authors.forename: Tuula
authors.initials: T
authors.lastname: Salo
authors.forename: Pirkko J
authors.initials: PJ
authors.lastname: Pussinen
authors.forename: Timur
authors.initials: T
authors.lastname: Köse
authors.forename: Gül
authors.initials: G
authors.lastname: Atilla
authors.forename: Timo
authors.initials: T
authors.lastname: Sorsa
mesh_terms: D000225:Adenine; D000293:Adolescent; D000328:Adult; D010520:Aggressive Periodontitis / Q000201:enzymology* / Q000235:genetics / Q000628:therapy; D016301:Alveolar Bone Loss / Q000201:enzymology / Q000628:therapy; D015415:Biomarkers / Q000032:analysis; D003596:Cytosine; D005260:Female; D005500:Follow-Up Studies; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D006147:Guanine; D006801:Humans; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000235:genetics*; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000201:enzymology / Q000628:therapy; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012737:Sex Factors; D013936:Thymidine; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics*; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D015415:Biomarkers; C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D006147:Guanine; D003596:Cytosine; C488762:MMP8 protein, human; D020784:Matrix Metalloproteinase 8; D000225:Adenine; D013936:Thymidine
keywords: Aggressive periodontitis; genes; gingival crevicular fluid; matrix metalloproteinase 8; polymorphism; tissue inhibitor of metalloproteinase-1