title: A sensitive UPLC-MS/MS method for simultaneous determination of eleven bioactive components of Tong-Xie-Yao-Fang decoction in rat biological matrices.
abstract: There is a growing concern for the sensitive quantification of multiple components using advanced data acquisition method in herbal medicines (HMs). An improved and rugged UPLC-MS/MS method has been developed and validated for sensitive and rapid determination of multiply analytes from Tong-Xie-Yao-Fang (TXYF) decoction in three biological matrices (plasma/brain tissue/urine) using geniposide and formononetin as internal standards. After solid-phase extraction, chromatographic separation was performed on a C18 column using gradient elution. Quantifier and qualifier transitions were monitored using novel Triggered Dynamic multiple reaction monitoring (TdMRM) in the positive ionization mode. A significant peak symmetry and sensitivity improvement in the TdMRM mode was achieved as compared to conventional MRM. The reproducibility (RSD%) was ≤7.9% by applying TdMRM transition while the values were 6.8-20.6% for MRM. Excellent linear calibration curves were obtained under TdMRM transitions over the tested concentration ranges. Intra- and inter-day precisions (RSD%) were ≤14.2% and accuracies (RE%) ranged from -9.6% to 10.6%. The validation data of specificity, carryover, recovery, matrix effect and stability were within the required limits. The method was effectively applied to simultaneously detect and quantify 1 lactone, 2 monoterpene glucosides, 1 alkaloid, 5 flavonoids and 2 chromones in plasma, brain tissue and urine after oral administration of TXYF decoction. In conclusion, this new and reliable method is beneficial for quantification and confirmation assays of multiply components in complex biological samples.
authors.affiliation: Laboratory of Pharmaceutics, Jiangsu Key laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, People's Republic of China.
authors.forename: Tian-xue
authors.initials: TX
authors.lastname: Li
authors.affiliation: Laboratory of Pharmaceutics, Jiangsu Key laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, People's Republic of China.
authors.forename: Lang
authors.initials: L
authors.lastname: Hu
authors.affiliation: Laboratory of Pharmaceutics, Jiangsu Key laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, People's Republic of China.
authors.forename: Meng-meng
authors.initials: MM
authors.lastname: Zhang
authors.affiliation: Laboratory of Pharmaceutics, Jiangsu Key laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.affiliation: Laboratory of Pharmaceutics, Jiangsu Key laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Laboratory of Pharmaceutics, Jiangsu Key laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, People's Republic of China.
authors.forename: Jun-qian
authors.initials: JQ
authors.lastname: Rui
authors.affiliation: Laboratory of Pharmaceutics, Jiangsu Key laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, People's Republic of China. Electronic address: yangxinh@126.com.
authors.forename: Xing-hao
authors.initials: XH
authors.lastname: Yang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000032:analysis* / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis / Q000097:blood / Q000302:isolation & purification / Q000652:urine; D057889:Iridoid Glycosides / Q000032:analysis / Q000097:blood / Q000302:isolation & purification / Q000652:urine; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D057889:Iridoid Glycosides
keywords: 4′-O-β-glucopyranosyl-5-O-methylvisamminol; Bio-analysis; Biological matrices; D-IBS; GMV; HM; Multi-component determination; SPE; TCHM; TXYF; TdMRM; Tong-Xie-Yao-Fang; Triggered Dynamic multiple reaction monitoring; UPLC–ESI–MS/MS; UPLC–MS/MS; diarrhea-predominant irritable bowel syndrome; herbal medicine; solid-phase extraction; traditional Chinese herbal medicine; ultra-high performance liquid chromatography–electrospray ionization–tandem mass spectrometry