title: Protective effect of total flavonoids extracted from the leaves of Murraya paniculata (L.) Jack on diabetic nephropathy in rats.
abstract: This study was designed to evaluate the effects of total flavonoids extracted from the leaves of Murraya paniculata (L.) Jack (TFMP) on diabetic nephropathy. High fat diet and streptozotocin-induced diabetic rats were treated with the TFMP (35 or 70 mg/kg) for 13 weeks. Changes of renal function parameters were examined at the end of administration. Some kidneys were collected for histological and immunohistochemistry studies, the other ones for biochemical parameters analysis. TFMP significantly decreased the levels of serum blood urea nitrogen, serum creatinine, creatinine clearance, interleukin-6, urinary albumin, 24h-urinary albumin excretion rate, kidney weight to body weight ratio and fasting blood glucose in diabetic rats. Meanwhile, the levels of triglycerides, total and LDL cholesterols in the TFMP treated diabetic rats were lower and the high-density lipoprotein cholesterol level was higher than that in the diabetic rats. TFMP treatment significantly blocked the decrease of superoxide dismutase and glutathione peroxidase and increase of malondialdehyde levels in diabetic rats. Furthermore, the TFMP not only decreased the expression of TGF-β1 and CTGF protein, but also reduced diabetes-induced morphological alterations of the kidney. These results suggest that TFMP is a protective agent against renal damage in diabetic nephropathy.
authors.affiliation: Department of Pharmacology, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Jingtao
authors.initials: J
authors.lastname: Zou
authors.affiliation: Department of Pharmacology, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Pharmacology, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Shaochun
authors.initials: S
authors.lastname: Qu
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130021, China.
authors.forename: Xuwen
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130021, China.
authors.forename: Yongri
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Pharmacology, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China. Electronic address: dayuansui@hotmail.com.
authors.forename: Dayuan
authors.initials: D
authors.lastname: Sui
mesh_terms: D000419:Albuminuria / Q000150:complications; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001806:Blood Urea Nitrogen; D008078:Cholesterol, LDL / Q000097:blood; D003404:Creatinine / Q000097:blood / Q000652:urine; D003928:Diabetic Nephropathies / Q000201:enzymology / Q000378:metabolism / Q000517:prevention & control*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D032101:Murraya / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D008078:Cholesterol, LDL; D005419:Flavonoids; D014280:Triglycerides; D003404:Creatinine; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Diabetic nephropathy; High fat diet; Murraya paniculata (L.) Jack; Rat; Renal damage; Streptozotocin