title: Laparoscopic sleeve gastrectomy ameliorates mRNA expression of inflammation-related genes in subcutaneous adipose tissue but not in peripheral monocytes of obese patients.
abstract: Low-grade inflammation links obesity, insulin resistance, and cardiovascular diseases. We investigated the effects of laparoscopic sleeve gastrectomy (LSG) on expression profile of genes involved in inflammatory pathways in subcutaneous adipose tissue (SCAT) and peripheral monocytes (PM). At baseline, obese group had significantly increased mRNA expression of proinflammatory chemokines (CCL-3, -17, -22), chemokine receptor CCR1 and cytokines (IL-10, IL-18) in SCAT and chemokine and other proinflammatory receptors (CCR-1, -2, -3, TLR-2, -4) in PM relative to control group. LSG decreased body weight, improved metabolic profile and reduced mRNA expression of up-regulated chemokine receptors, chemokines and cytokines in SCAT. In contrast, expression profiles in PM were largely unaffected by LSG. We conclude that LSG improved proinflammatory profile in subcutaneous fat but not in peripheral monocytes. The sustained proinflammatory and chemotactic profile in PM even 2 years after LSG may contribute to partial persistence of metabolic complications in obese patients after metabolic surgery.
authors.affiliation: Third Department of Medicine, 1st Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic.
authors.forename: P
authors.initials: P
authors.lastname: Trachta
authors.affiliation: Third Department of Medicine, 1st Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic.
authors.forename: I
authors.initials: I
authors.lastname: Dostálová
authors.affiliation: Third Department of Medicine, 1st Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic; Department of Sports Medicine, 1st Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic.
authors.forename: D
authors.initials: D
authors.lastname: Haluzíková
authors.affiliation: Department of Surgery, 2nd Faculty of Medicine, Charles University and Military University Hospital, Prague, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Kasalický
authors.affiliation: Third Department of Medicine, 1st Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic.
authors.forename: P
authors.initials: P
authors.lastname: Kaválková
authors.affiliation: Third Department of Medicine, 1st Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Drápalová
authors.affiliation: Third Department of Medicine, 1st Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Urbanová
authors.affiliation: Third Department of Medicine, 1st Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic.
authors.forename: Z
authors.initials: Z
authors.lastname: Lacinová
authors.affiliation: Third Department of Medicine, 1st Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Mráz
authors.affiliation: Third Department of Medicine, 1st Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic. Electronic address: mhalu@lf1.cuni.cz.
authors.forename: M
authors.initials: M
authors.lastname: Haluzík
mesh_terms: D000328:Adult; D019742:Chemokines, CC / Q000235:genetics / Q000378:metabolism; D005260:Female; D005743:Gastrectomy / Q000379:methods*; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D020382:Interleukin-18 / Q000235:genetics / Q000378:metabolism; D010535:Laparoscopy; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism* / Q000473:pathology; D009765:Obesity / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D054388:Receptors, CCR / Q000235:genetics / Q000378:metabolism; D050151:Subcutaneous Fat / Q000378:metabolism* / Q000473:pathology; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism; D015431:Weight Loss
chemical_list: D019742:Chemokines, CC; C508609:IL10 protein, human; D020382:Interleukin-18; D012333:RNA, Messenger; D054388:Receptors, CCR; C495342:TLR2 protein, human; C495345:TLR4 protein, human; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D016753:Interleukin-10
keywords: Adipose tissue; Laparoscopic sleeve gastrectomy; Obesity; Peripheral monocytes