title: Digitally enhanced thin layer chromatography: further development and some applications in isotopic chemistry.
abstract: Improvements to thin layer chromatography (TLC) analysis can be made easily and cheaply by the application of digital colour photography and image analysis. The combined technique, digitally enhanced TLC (DE-TLC), is applicable to the accurate quantification of analytes in mixtures, to reaction monitoring and to other typical uses of TLC. Examples are given of the application of digitally enhanced TLC to: the deuteromethylations of theophylline to [methyl-(2)H3]caffeine and of umbelliferone to [(2)H3]7-methoxycoumarin; the selection of tertiary amine bases in deuterodechlorination reactions; stoichiometry optimisation in the borodeuteride reduction of quinizarin (1,4-dihydroxyanthraquinone) and to the assessment of xanthophyll yields in Lepidium sativum seedlings grown in deuterated media.
authors.affiliation: Department of Chemistry, Faculty of Engineering and Physical Sciences, University of Surrey, Guildford, GU2 7XH, UK.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Manthorpe
authors.forename: William J S
authors.initials: WJ
authors.lastname: Lockley
mesh_terms: D000880:Anthraquinones / Q000032:analysis; D002110:Caffeine / Q000031:analogs & derivatives / Q000032:analysis; D002338:Carotenoids / Q000032:analysis; D002855:Chromatography, Thin Layer / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007553:Isotope Labeling / Q000379:methods; D031220:Lepidium sativum / Q000737:chemistry; D012680:Sensitivity and Specificity; D013806:Theophylline / Q000032:analysis; D014316:Tritium / Q000032:analysis*
chemical_list: C039577:2-methylcaffeine; D000880:Anthraquinones; D014316:Tritium; D002338:Carotenoids; D002110:Caffeine; C034890:1,4-dihydroxyanthraquinone; D013806:Theophylline
keywords: DE-TLC; Lepidium sativum carotenoids; [2H3]7-methoxycoumarin; [9,10−2H2]1,4-anthraquinone; deuteromethylation; digitally enhanced TLC; methyl−2H3]caffeine; quinizarin; umbelliferone