title: Cloning of a Family 11 Xylanase Gene from Bacillus amyloliquefaciens CH51 Isolated from Cheonggukjang.
abstract: Bacillus amyloliquefaciens CH51, an isolate from cheonggukjang, Korean fermented soyfood, secretes several enzymes into culture medium. A gene encoding 19 kDa xylanase was cloned by PCR. Sequencing showed that the gene encoded a glycohydrolase family 11 xylanase and named xynA. xynAHis, xynA with additional codons for his-tag, was overexpressed in Escherichia coli BL21(DE3) using pET-26b(+). XynAHis was purified using HisTrap affinity column. Km and Vmax of XynAHis were 0.363 mg/ml and 701.1 μmol/min/mg, respectively with birchwood xylan as a substrate. The optimum pH and temperature were pH 4 and 25 °C, respectively. When xynA was introduced into Bacillus subtilis WB600, active XynA was secreted into culture medium.
authors.affiliation: Division of Applied Life Science (Bk21), Graduate School, Research Institute of Life Sciences, Gyeongsang National University, Jinju, Korea.
authors.forename: C U
authors.initials: CU
authors.lastname: Baek
authors.forename: S G
authors.initials: SG
authors.lastname: Lee
authors.forename: Y R
authors.initials: YR
authors.lastname: Chung
authors.forename: I
authors.initials: I
authors.lastname: Cho
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
keywords: Bacillus amyloliquefaciens; Family 11 xylanase; Overexpression; Purification