title: Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells.
abstract: Low vitality (a component of fatigue) in middle-aged and older adults is an important complaint often identified as a symptom of a disease state or side effect of a treatment. No studies to date have investigated the potential link between dysfunctional mitochondrial ATP production and low vitality. Therefore, we measured a number of cellular parameters related to mitochondrial activity in peripheral blood mononuclear cells (PBMCs) isolated from middle-aged men, and tested for association with vitality. These parameters estimate mitochondrial respiration, reactive oxygen species (ROS) production, and deoxyribonucleotide (dNTP) balance in PBMCs. The population was drawn from the Metropolit cohort of men born in 1953. Vitality level was estimated from the Medical Outcomes Study Short Form 36 (SF-36) vitality scale. We found that vitality score had no association with any of the mitochondrial respiration parameters. However, vitality score was inversely associated with cellular ROS production and cellular deoxythymidine triphosphate (dTTP) levels and positively associated with deoxycytidine triphosphate (dCTP) levels. We conclude that self-reported persistent low vitality is not associated with specific aspects of mitochondrial oxidative phosphorylation capacity in PBMCs, but may have other underlying cellular dysfunctions that contribute to dNTP imbalance and altered ROS production.
authors.affiliation: Center for Healthy Aging at the University of Copenhagen, 2200 Copenhagen N, Denmark;
authors.forename: Scott
authors.initials: S
authors.lastname: Maynard
authors.forename: Guido
authors.initials: G
authors.lastname: Keijzers
authors.forename: Martin
authors.initials: M
authors.lastname: Gram
authors.forename: Claus
authors.initials: C
authors.lastname: Desler
authors.forename: Laila
authors.initials: L
authors.lastname: Bendix
authors.forename: Esben
authors.initials: E
authors.lastname: Budtz-Jørgensen
authors.forename: Drude
authors.initials: D
authors.lastname: Molbo
authors.forename: Deborah L
authors.initials: DL
authors.lastname: Croteau
authors.forename: Merete
authors.initials: M
authors.lastname: Osler
authors.forename: Tinna
authors.initials: T
authors.lastname: Stevnsner
authors.forename: Lene Juel
authors.initials: LJ
authors.lastname: Rasmussen
authors.forename: Flemming
authors.initials: F
authors.lastname: Dela
authors.forename: Kirsten
authors.initials: K
authors.lastname: Avlund
authors.forename: Vilhelm A
authors.initials: VA
authors.lastname: Bohr
mesh_terms: D019069:Cell Respiration; D003854:Deoxyribonucleotides / Q000378:metabolism*; D005221:Fatigue / Q000209:etiology*; D006019:Glycolysis; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism*; D010101:Oxygen Consumption; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D003854:Deoxyribonucleotides; D017382:Reactive Oxygen Species