title: Dual-treatment autodissemination station with enhanced transfer of an insect growth regulator to mosquito oviposition sites.
abstract: BACKGROUND
  The Asian tiger mosquito, Aedes albopictus (Skuse), transmits important arboviral diseases and displaces native species. This peridomestic mosquito deposits eggs in natural and artificial containers. Container larval habitats tend to be cryptic and, therefore, difficult to reach by conventional insecticide treatments. We have developed an autodissemination station that transfers the insect growth regulator, pyriproxyfen, from the station to oviposition sites. Mosquitoes visiting the station become contaminated with an oil sticker followed by pyriproxyfen powder before exiting.
  
  
  RESULTS
  In a room (31 m(3) ) bioassay a single station consistently achieved 100% Ae. albopictus pupal mortality against 10 oviposition containers. In a greenhouse (200 m(3) ) assay with two stations and 12 oviposition cups, 91.7% of the cups had been contaminated as shown, and 57.1% pupal mortality was recorded. Pyriproxyfen transfer was also detected by visualizing mosquito 'tarsal prints' using a fluorescent dye.
  
  
  CONCLUSIONS
  The oil and pyriproxyfen powder dual-treatment station enhanced autodissemination efficacy by increasing toxicant attachment and retention on contaminated females. The autodissemination station offers a targeted, less environmentally damaging approach to manage cryptic container species.
authors.affiliation: Center for Vector Biology, Rutgers University, New Brunswick, NJ, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Devi S
authors.initials: DS
authors.lastname: Suman
authors.forename: Jacques
authors.initials: J
authors.lastname: Bertrand
authors.forename: Limin
authors.initials: L
authors.lastname: Dong
authors.forename: Randy
authors.initials: R
authors.lastname: Gaugler
mesh_terms: D000330:Aedes / Q000187:drug effects*; D000818:Animals; D005260:Female; D007605:Juvenile Hormones / Q000008:administration & dosage* / Q000494:pharmacology; D009032:Mosquito Control / Q000295:instrumentation / Q000379:methods*; D010058:Oviposition / Q000502:physiology; D011679:Pupa / Q000187:drug effects*; D011725:Pyridines / Q000008:administration & dosage* / Q000494:pharmacology*; D014867:Water / Q000737:chemistry
chemical_list: D007605:Juvenile Hormones; D011725:Pyridines; D014867:Water; C055613:pyriproxyfen
keywords: Aedes albopictus; autodissemination; container mosquito; insect growth regulator; pyriproxyfen