title: The effect of different enamel surface treatments on microleakage of fissure sealants.
abstract: OBJECTIVE
  The aim of this study was to evaluate the effects of different techniques of surface treatment on the microleakage of fissure sealants in permanent molar teeth in vitro.
  
  
  METHODS
  96 extracted impacted human third molars were randomly divided into 8 surface treatment groups (n=12/group) as 1. Er: YAG laser ; (Fidelis II, Fotona, Ljubljana, Slovenia) (125 mj, 20Hz) ; 2. Er: YAG laser + 37% H3PO4 (15s) ; 3. ER: AG laser + 37% H3PO4+Prime&Bond NT ; 4. Er: YAG laser + G Bond ; 5. Er: YAG laser + Prime&Bond NT ; 6. 37% H3PO4 ; 7. 37% H3PO4 + Prime&Bond NT ; 8. G Bond. Sealant material (Clinpro, 3M ESPE, Seefeld, Germany), was applied into the fissures and light-cured for 20s with LED (Bluephase C5, Ivoclar-Vivadent, Schaan, Liechtenstein). Specimens were subjected to thermocycling (1000×, 5-55°C, dwell time: 15s) and immersed in 0.5% basic fuchsin solution for 24h at 37°C. The samples were sectioned and scored on a 3 point rating scale using a light microscope with a magnification of ×20. One-way analysis of variance was used to analyze data. Multiple comparisons were analyzed using Bonferroni test (p=0.05).
  
  
  RESULTS
  Er:YAG laser showed the highest microleakage scores whereas Er YAG laser + 37% H3PO4 showed the lowest. Although 37% H3PO4 group showed higher scores than Er:YAG laser + 37% H3PO4, the difference was not statistically significant.
  
  
  CONCLUSIONS
  Etching fissures with phosphoric acid is sufficient prior to fissure sealant application.
authors.affiliation: Ege University, School of Dentistry, Pedodontics Department, Bornova, Izmir, Turkey, PK: 35100. aslitopaloglu@yahoo.com.
authors.forename: Asli
authors.initials: A
authors.lastname: Topaloglu-Ak
authors.forename: Ozant
authors.initials: O
authors.lastname: Onçağ
authors.forename: Bülent
authors.initials: B
authors.lastname: Gökçe
authors.forename: Berrin
authors.initials: B
authors.lastname: Bent
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods*; D003743:Dental Enamel; D003750:Dental Fissures / Q000628:therapy*; D003763:Dental Leakage / Q000517:prevention & control*; D005260:Female; D006801:Humans; D053685:Laser Therapy / Q000009:adverse effects; D008297:Male; D008964:Molar, Third; D010756:Phosphoric Acids / Q000008:administration & dosage; D010895:Pit and Fissure Sealants / Q000009:adverse effects / Q000627:therapeutic use*; D013499:Surface Properties
chemical_list: D010756:Phosphoric Acids; D010895:Pit and Fissure Sealants; C030242:phosphoric acid