title: Lupeol is one of active components in the extract of Chrysanthemum indicum Linne that inhibits LMP1-induced NF-κB activation.
abstract: We have previously reported that seventy percent ethanol extract of Chrysanthemum indicum Linne (CIE) strongly reduces Epstein-Barr virus (EBV)-transformed lymphoblastoid cell line (LCL) survival by inhibiting virus-encoded latent infection membrane protein 1 (LMP1)-induced NF-κB activation. To identify an active compound(s) in CIE that inhibits LMP1-induced NF-κB activation, activity-guided fractionation was employed. The CH2Cl2 fraction of CIE strongly reduced LMP1-induced NF-κB activation and LCL viability with relatively low cytotoxic effects on primary human foreskin fibroblast (HFF), HeLa or Burkitt's lymphoma (BL41) cells. Furthermore, lupeol, a pentacyclic triterpene, was identified in the CH2Cl2 fraction of CIE to attenuate LMP1-induced NF-κB activation and LCL viability. This study demonstrates that lupeol is one of active compounds in the CH2Cl2 fraction of CIE that inhibits LMP1-induced NF-κB activation and reduces NF-κB-dependent LCL viability.
authors.affiliation: Department of Life Science, Gachon University, Seongnam-Si, Kyeonggi-Do, Republic of Korea.
authors.forename: Se Chan
authors.initials: SC
authors.lastname: Kang
authors.forename: Sue Yeon
authors.initials: SY
authors.lastname: Lim
authors.forename: Yoon-Jae
authors.initials: YJ
authors.lastname: Song
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects; D005591:Chemical Fractionation; D031202:Chrysanthemum / Q000737:chemistry*; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D051550:I-kappa B Kinase / Q000378:metabolism; D020128:Inhibitory Concentration 50; D060588:LIM Domain Proteins / Q000378:metabolism*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D053978:Pentacyclic Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry*; D000094042:Lupanes
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; D051550:I-kappa B Kinase; D060588:LIM Domain Proteins; D016328:NF-kappa B; D053978:Pentacyclic Triterpenes; D010936:Plant Extracts; C484380:PDLIM7 protein, human; C010480:lupeol; D000094042:Lupanes