title: The essential role of the phosphorylated pathway of serine biosynthesis in Arabidopsis.
abstract: In plants, 3 different pathways of serine biosynthesis have been described: the Glycolate pathway, which is associated with photorespiration, and 2 non-photorespiratory pathways, the Glycerate and the Phosphorylated pathways. The Phosphorylated Pathway of Serine Biosynthesis (PPSB) has been known since the 1950s, but has been studied relatively little, probably because it was considered of minor significance as compared with the Glycolate pathway. In the associated study (1), we described for the first time in plants the in vivo functional characterization of the PPSB, by targeting the phosphoserine phosphatase (PSP1), the last enzyme of the pathway. Following a gain- and loss-of-function approach in Arabidopsis, we provided genetic and molecular evidence for the essential role of PSP1 for embryo and pollen development, and for proper root growth. A metabolomics study indicated that the PPSB affects glycolysis, the Krebs cycle, and the biosynthesis of several amino acids, which suggests that this pathway is an important link connecting metabolism and development. The mechanisms underlying the essential functions of PSP1 are discussed.
authors.affiliation: ERI de Biotecnologia i Biomedicina; Departament de Biologia Vegetal; Facultat de Farmàcia; Universitat de València; Burjassot, Valencia, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Muñoz-Bertomeu
authors.affiliation: ERI de Biotecnologia i Biomedicina; Departament de Biologia Vegetal; Facultat de Farmàcia; Universitat de València; Burjassot, Valencia, Spain.
authors.forename: Armand
authors.initials: A
authors.lastname: Anoman
authors.affiliation: ERI de Biotecnologia i Biomedicina; Departament de Biologia Vegetal; Facultat de Farmàcia; Universitat de València; Burjassot, Valencia, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Flores-Tornero
authors.affiliation: ERI de Biotecnologia i Biomedicina; Departament de Biologia Vegetal; Facultat de Farmàcia; Universitat de València; Burjassot, Valencia, Spain.
authors.forename: Walid
authors.initials: W
authors.lastname: Toujani
authors.affiliation: ERI de Biotecnologia i Biomedicina; Departament de Biologia Vegetal; Facultat de Farmàcia; Universitat de València; Burjassot, Valencia, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Rosa-Téllez
authors.affiliation: Max Planck Institut für Molekulare Pflanzenphysiologie; Potsdam-Golm, Germany.
authors.forename: Alisdair R
authors.initials: AR
authors.lastname: Fernie
authors.affiliation: Institute of Biological Chemistry; Washington State University; Pullman, WA USA.
authors.forename: Sanja
authors.initials: S
authors.lastname: Roje
authors.affiliation: ERI de Biotecnologia i Biomedicina; Departament de Biologia Vegetal; Facultat de Farmàcia; Universitat de València; Burjassot, Valencia, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Segura
authors.affiliation: ERI de Biotecnologia i Biomedicina; Departament de Biologia Vegetal; Facultat de Farmàcia; Universitat de València; Burjassot, Valencia, Spain.
authors.forename: Roc
authors.initials: R
authors.lastname: Ros
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics; D053898:Biosynthetic Pathways; D049452:Green Fluorescent Proteins / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics; D010766:Phosphorylation; D012694:Serine / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D049452:Green Fluorescent Proteins; D012694:Serine; D010744:Phosphoric Monoester Hydrolases; C586768:PSP1 protein, Arabidopsis
keywords: male gametophyte; phosphorylated pathway of serine biosynthesis; phosphoserine phosphatase; root and embryo development