title: Inhibition of the growth of colorado potato beetle larvae by macrocypins, protease inhibitors from the parasol mushroom.
abstract: Proteins from higher fungi have attracted interest because of their exceptional characteristics. Macrocypins, cysteine protease inhibitors from the parasol mushroom Macrolepiota procera , were evaluated for their adverse effects and their mode of action on the major potato pest Colorado potato beetle (CPB, Leptinotarsa decemlineata Say). They were shown to reduce larval growth when expressed in potato or when their recombinant analogues were added to the diet. Macrocypins target a specific set of digestive cysteine proteases, intestains. Additionally, protein-protein interaction analysis revealed potential targets among other digestive enzymes and proteins related to development and primary metabolism. No effect of dietary macrocypins on gene expression of known adaptation-related digestive enzymes was observed in CPB guts. Macrocypins are the first fungal protease inhibitors to be reported as having a negative effect on growth and development of CPB larvae and could also be evaluated as control agents for other pests.
authors.affiliation: Department of Biotechnology and Systems Biology, National Institute of Biology , Večna pot 111, 1000 Ljubljana, Slovenia.
authors.forename: Ida
authors.initials: I
authors.lastname: Smid
authors.forename: Kristina
authors.initials: K
authors.lastname: Gruden
authors.forename: Meti
authors.initials: M
authors.lastname: Buh Gašparič
authors.forename: Katarina
authors.initials: K
authors.lastname: Koruza
authors.forename: Marko
authors.initials: M
authors.lastname: Petek
authors.forename: Jure
authors.initials: J
authors.lastname: Pohleven
authors.forename: Jože
authors.initials: J
authors.lastname: Brzin
authors.forename: Janko
authors.initials: J
authors.lastname: Kos
authors.forename: Jana
authors.initials: J
authors.lastname: Zel
authors.forename: Jerica
authors.initials: J
authors.lastname: Sabotič
mesh_terms: D000363:Agaricales / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001517:Coleoptera / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D019476:Insect Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D007814:Larva / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D010447:Peptide Hydrolases / Q000235:genetics / Q000378:metabolism; D010935:Plant Diseases / Q000469:parasitology / Q000517:prevention & control*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000469:parasitology*; D011480:Protease Inhibitors / Q000378:metabolism*; D011198:Solanum tuberosum / Q000235:genetics / Q000378:metabolism / Q000469:parasitology*
chemical_list: D005656:Fungal Proteins; D019476:Insect Proteins; D011480:Protease Inhibitors; D010447:Peptide Hydrolases