title: MicroRNA-30b functions as a tumour suppressor in human colorectal cancer by targeting KRAS, PIK3CD and BCL2.
abstract: Colorectal cancer (CRC) is the third most common cancer in the USA. MicroRNAs play important roles in the pathogenesis of CRC. In this study, we investigated the role of miR-30b in CRC and found that its expression was significantly lower in CRC tissues than that in normal tissues. We showed that a low expression level of miR-30b was closely related to poor differentiation, advanced TNM stage and poor prognosis of CRC. Further experiments showed that over-expression of miR-30b suppressed CRC cell proliferation in vitro and tumour growth in vivo. Specifically, miR-30b promoted G1 arrest and induced apoptosis. Moreover, KRAS, PIK3CD and BCL2 were identified as direct and functional targets of miR-30b. MiR-30b directly targeted the 3'-untranslated regions of their mRNAs and repressed their expression. This study revealed functional and mechanistic links between miRNA-30b and oncogene KRAS, PIK3CD and BCL2 in the pathogenesis of CRC. MiR-30b not only plays important roles in the regulation of cell proliferation and tumour growth in CRC, but is also a potential prognostic marker or therapeutic target for CRC. Restoration of miR-30b expression may represent a promising therapeutic approach for targeting malignant CRC.
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, People's Republic of China; Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.
authors.forename: Wen-Ting
authors.initials: WT
authors.lastname: Liao
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Ye
authors.forename: Nian-Jie
authors.initials: NJ
authors.lastname: Zhang
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Li
authors.forename: Shu-Yang
authors.initials: SY
authors.lastname: Wang
authors.forename: Yan-Mei
authors.initials: YM
authors.lastname: Cui
authors.forename: Lu
authors.initials: L
authors.lastname: Qi
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
authors.forename: Hong-Li
authors.initials: HL
authors.lastname: Jiao
authors.forename: Yi-Jun
authors.initials: YJ
authors.lastname: Xie
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.forename: Jun-Xian
authors.initials: JX
authors.lastname: Wang
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Ding
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D017209:Apoptosis; D001665:Binding Sites; D002454:Cell Differentiation; D049109:Cell Proliferation; D058534:Class I Phosphatidylinositol 3-Kinases; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D019295:Computational Biology; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D045325:HCT116 Cells; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009367:Neoplasm Staging; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D016283:Proto-Oncogene Proteins p21(ras); D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection; D047368:Tumor Burden; D018631:ras Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; D058534:Class I Phosphatidylinositol 3-Kinases; D035683:MicroRNAs; D019869:Phosphatidylinositol 3-Kinases; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016283:Proto-Oncogene Proteins p21(ras); D012333:RNA, Messenger; D018631:ras Proteins; C117307:KRAS protein, human; C546086:MIRN30b microRNA, human; C486018:PIK3CD protein, human
keywords: BCL2; KRAS; PIK3CD; apoptosis; colorectal cancer; miR-30b; proliferation