title: Fuzzy clustering analysis of osteosarcoma related genes.
abstract: Osteosarcoma is the most common malignant bone-tumor with a peak manifestation during the second and third decade of life. In order to explore the influence of genetic factors on the mechanism of osteosarcoma by analyzing the inter relationship between osteosarcoma and its related genes, and then provide potential genetic references for the prevention, diagnosis and treatment of osteosarcoma, we collected osteosarcoma related gene sequences in Genebank of National Center for Biotechnology Information (NCBI) and local alignment analysis for a pair of sequences was carried out to identify the measurement association among related sequences. Then fuzzy clustering method was used for clustering analysis so as to contact the unknown genes through the consistent osteosarcoma related genes in one class. From the result of fuzzy clustering analysis, we could classify the osteosarcoma related genes into two groups and deduced that the genes clustered into one group had similar function. Based on this knowledge, we found more genes related to the pathogenesis of osteosarcoma and these genes could exert similar function as Runx2, a risk factor confirmed in osteosarcoma, this study may help better understand the genetic mechanism and provide new molecular markers and therapies for osteosarcoma.
authors.affiliation: Department of Orthopedics, Changhai Hospital, Shanghai, 200433, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.forename: Dajiang
authors.initials: D
authors.lastname: Wu
authors.forename: Yushu
authors.initials: Y
authors.lastname: Bai
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhu
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Chen
authors.forename: Chuanfeng
authors.initials: C
authors.lastname: Wang
authors.forename: Yingchuan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Ming
authors.initials: M
authors.lastname: Li
mesh_terms: D000465:Algorithms; D001859:Bone Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000628:therapy; D016000:Cluster Analysis; D030541:Databases, Genetic; D003936:Diagnosis, Computer-Assisted / Q000706:statistics & numerical data; D017143:Fuzzy Logic; D006801:Humans; D058977:Molecular Sequence Annotation; D005810:Multigene Family; D012516:Osteosarcoma / Q000175:diagnosis / Q000235:genetics* / Q000628:therapy