title: The effects of microenvironment on wound healing by keratinocytes derived from mesenchymal stem cells.
abstract: Embryonic stem cells (ESCs) are pluripotent cells that can differentiate into various cell types, including keratinocyte-like cells, within suitable microniches. In this study, we aimed to investigate the effects of culture media, cell coculture, and a tissue-engineering biocomposite on the differentiation of mouse ESCs (MESCs) into keratinocyte-like cells and applied these cells to a surgical skin wound model. MESCs from BALB/c mice (ESC26GJ), which were transfected using pCX-EGFP expressing green fluorescence, were used to track MESC-derived keratinocytes. Weak expression of the keratinocyte early marker Cytokeratin 14 (CK-14) was observed up to 12 days when MESCs were cultured in a keratinocyte culture medium on tissue culture plastic and on a gelatin/collagen/polycaprolactone (GCP) biocomposite. MESCs cocultured with human keratinocyte cells (HKCs) also expressed CK-14, but did not express CK-14 when cocultured with human fibroblast cells (HFCs). Furthermore, CK-14 expression was observed when MESCs were cocultured by seeding HKCs or HFCs on the same or opposite side of the GCP biocomposite. The highest CK-14 expression was observed by seeding MESCs and HKCs on the same side of the GCP composite and with HFCs on the opposite side. To verify the effectiveness of wound healing in vivo, adipose-derived stem cells were applied to treat surgical wounds in nude mice. An obvious epidermis multilayer and better collagen deposition during wound healing were observed, as assessed by Masson staining. This study demonstrated the potential of keratinocyte-like differentiation from mesenchymal stem cells for use in promoting wound closure and skin regeneration.
authors.affiliation: From the *Biomedical Engineering Program, Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology; †Division of Plastic and Reconstructive Surgery, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center; ‡Division of Plastic Surgery, Veteran General Hospital; §Institute of Biology and Anatomy, and ∥Graduate Institute of Aerospace and Undersea Medicine, National Defense Medical Center, Taipei; ¶Department of Health-Business Administration, Hungkuang University; #Department of Orthopedics, Kuang-Tien General Hospital, Taichung; and **Graduate Institute of Medical Sciences, National Defense Medical Center, Taiwan, Republic of China.
authors.forename: Yi-Han
authors.initials: YH
authors.lastname: Lin
authors.forename: Keng-Yen
authors.initials: KY
authors.lastname: Fu
authors.forename: Po-Da
authors.initials: PD
authors.lastname: Hong
authors.forename: Hsu
authors.initials: H
authors.lastname: Ma
authors.forename: Nien-Hsien
authors.initials: NH
authors.lastname: Liou
authors.forename: Kuo-Hsing
authors.initials: KH
authors.lastname: Ma
authors.forename: Jiang-Chuan
authors.initials: JC
authors.lastname: Liu
authors.forename: Kun-Lun
authors.initials: KL
authors.lastname: Huang
authors.forename: Lien-Guo
authors.initials: LG
authors.lastname: Dai
authors.forename: Shun-Cheng
authors.initials: SC
authors.lastname: Chang
authors.forename: James
authors.initials: J
authors.lastname: Yi-Hsin Chan
authors.forename: Shyi-Gen
authors.initials: SG
authors.lastname: Chen
authors.forename: Tim-Mo
authors.initials: TM
authors.lastname: Chen
authors.forename: Niann-Tzyy
authors.initials: NT
authors.lastname: Dai
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D018920:Coculture Techniques; D003470:Culture Media; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000502:physiology; D051379:Mice; D008819:Mice, Nude; D023822:Tissue Engineering / Q000379:methods*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D003470:Culture Media