title: Collagen implants equipped with 'fish scale'-like nanoreservoirs of growth factors for bone regeneration.
abstract: Implants triggering rapid, robust and durable tissue regeneration are needed to shorten recovery times and decrease risks of postoperative complications for patients. Here, we describe active living collagen implants with highly promising bone regenerative properties. Bioactivity of the implants is obtained through the protective and stabilizing layer-by-layer immobilization of a protein growth factor in association with a polysaccharide (chitosan), within the form of nanocontainers decorating the collagen nanofibers. All components of the implants are US FDA approved. From both in vitro and in vivo evaluations, the sophisticated strategy described here should enhance, at a reduced cost, the safety and efficacy of the therapeutic implants in terms of large bone defects repair compared with current simplistic approaches based on the soaking of the implants with protein growth factor.
authors.affiliation: INSERM, French National Institute of Health & Medical Research, Osteoarticular & Dental Regenerative Nanomedicine team, UMR1109, Faculté de Médecine, F-67085 Strasbourg Cedex, France.
authors.forename: Sandy
authors.initials: S
authors.lastname: Eap
authors.forename: Alice
authors.initials: A
authors.lastname: Ferrand
authors.forename: Jessica
authors.initials: J
authors.lastname: Schiavi
authors.forename: Laetitia
authors.initials: L
authors.lastname: Keller
authors.forename: Tunay
authors.initials: T
authors.lastname: Kokten
authors.forename: Florence
authors.initials: F
authors.lastname: Fioretti
authors.forename: Didier
authors.initials: D
authors.lastname: Mainard
authors.forename: Guy
authors.initials: G
authors.lastname: Ladam
authors.forename: Nadia
authors.initials: N
authors.lastname: Benkirane-Jessel
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage*; D001861:Bone Regeneration; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D008297:Male; D008819:Mice, Nude; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D055396:Bone Morphogenetic Protein 2; D003094:Collagen
keywords: bone morphogenetic protein 2; bone regeneration; collagen implant; drug delivery; layer-by-layer deposition; regenerative nanomedicine