title: Methanolic Extract of Asterina pectinifera inhibits LPS-Induced Inflammatory Mediators in Murine Macrophage.
abstract: This study aimed to elucidate anti-inflammatory activities from extracts of Asterina pectinifera on nitric oxide (NO) production, TNF-α and IL-6 release in lipopolysaccharide (LPS) -stimulated murine macrophage cell, RAW264.7. We prepared the methanolic extracts (60-MAP, 70-MAP, 80-MAP and 90-MAP) , aqueous extract (W-AP) and functional bioactive compound fraction (He-AP and EA-AP) from Asterina pectinifera according to extract method. The 60-MAP, 70-MAP, 80-MAP, 90-MAP and W-AP were significantly suppressed LPS-induced production NO, TNF-α and IL-6 secretion in a concentration-dependent manner (P < 0.05) . Especially, 80-MAP by extracted 80% methanol had the strongest activity in reduction of inflammatory mediators among these extracts. Indeed, to identify active fraction, which contained potential bioactive compounds, from 80-MAP of Asterina pectinifera, we tested anti-inflammatory activity of the He-AP or the EA-AP. The He-AP was next extracted from 80-MAP and the EA-AP were extracted from the other methanol layer except the He-AP. The EA-AP demonstrated a strong anti-inflammatory effect through its ability to reduce NO production and it also inhibited the production of proinflammatory cytokines such as IL-6 and TNF-α at low concentration. These results suggested that the methanolic extract from Asterina pectinifera had the potential inhibitory effects on the production of these inflammatory mediators.
authors.affiliation: Department of Microbiology, Dong-A University College of Medicine, Busan 602-714.
authors.forename: Wol-Soon
authors.initials: WS
authors.lastname: Jo
authors.forename: Yoo Jin
authors.initials: YJ
authors.lastname: Choi
authors.forename: Hyoun Ji
authors.initials: HJ
authors.lastname: Kim
authors.forename: Byung Hyouk
authors.initials: BH
authors.lastname: Nam
authors.forename: Gye An
authors.initials: GA
authors.lastname: Lee
authors.forename: Su Yeong
authors.initials: SY
authors.lastname: Seo
authors.forename: Sang Wha
authors.initials: SW
authors.lastname: Lee
authors.forename: Min Ho
authors.initials: MH
authors.lastname: Jeong
keywords: Antiinflammatory activity; Aqueous extract; Asterina pectinifera; LPS; Methanolic extract; Nitric oxide; Pro-inflammatory cytokines; RAW 264.7 cell