title: A Shortcut to the Optimization of Cellulase Production Using the Mutant Trichoderma reesei YC-108.
abstract: Trichoderma reesei YC-108, a strain isolated by a kind of newly invented plate was found to over produce cellulase and it was then used as a cellulase producer. To get the maximum amount of cellulase, the combination of the medium ingredients, which has a profound influence on metabolic pathway was optimized using response surface methodology. The optimum composition was found to be 24.63 g/L wheat bran, 30.78 g/L avicel, and 19.16 g/L soya-bean cake powder. By using the optimized medium, the filter paper activity (FPA) increased nearly five times to 15.82 IU/mL in a 30 L stirred fermenter, carboxymethyl cellulase activity (CMCase) was increased from 83.02 to 628.05 IU/mL and the CMCase/FPA ratio was nearly doubled compared with the parent strain at initial medium.
authors.affiliation: The Research Centre of Modern Analysis Technology, Tianjin University of Science & Technology, Tianjin, 300457 People's Republic China.
authors.forename: Zhong-Li
authors.initials: ZL
authors.lastname: Yan
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Cao
authors.forename: Qing-Dai
authors.initials: QD
authors.lastname: Liu
authors.forename: Zhi-Yan
authors.initials: ZY
authors.lastname: Yang
authors.forename: Yu-Ou
authors.initials: YO
authors.lastname: Teng
authors.forename: Juan
authors.initials: J
authors.lastname: Zhao
keywords: Cellulase; Optimization; RSM; Trichoderma reesei YC-108