title: Peptide conjugation via CuAAC 'click' chemistry.
abstract: The copper (I)-catalyzed alkyne azide 1,3-dipolar cycloaddition (CuAAC) or 'click' reaction, is a highly versatile reaction that can be performed under a variety of reaction conditions including various solvents, a wide pH and temperature range, and using different copper sources, with or without additional ligands or reducing agents. This reaction is highly selective and can be performed in the presence of other functional moieties. The flexibility and selectivity has resulted in growing interest in the application of CuAAC in various fields. In this review, we briefly describe the importance of the structural folding of peptides and proteins and how the 1,4-disubstituted triazole product of the CuAAC reaction is a suitable isoster for an amide bond. However the major focus of the review is the application of this reaction to produce peptide conjugates for tagging and targeting purpose, linkers for multifunctional biomacromolecules, and reporter ions for peptide and protein analysis.
authors.affiliation: School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, QLD 4072, Australia. i.toth@uq.edu.au.
authors.forename: Abdullah A H
authors.initials: AA
authors.lastname: Ahmad Fuaad
authors.forename: Fazren
authors.initials: F
authors.lastname: Azmi
authors.forename: Mariusz
authors.initials: M
authors.lastname: Skwarczynski
authors.forename: Istvan
authors.initials: I
authors.lastname: Toth
mesh_terms: D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D057930:Click Chemistry / Q000379:methods*; D003300:Copper / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry
chemical_list: D000480:Alkynes; D001386:Azides; D010455:Peptides; D011506:Proteins; D003300:Copper