title: Latent Transforming Growth Factor-beta1 Functionalised Electrospun Scaffolds Promote Human Cartilage Differentiation: Towards an Engineered Cartilage Construct.
abstract: BACKGROUND
  To overcome the potential drawbacks of a short half-life and dose-related adverse effects of using active transforming growth factor-beta 1 for cartilage engineering, a cell-mediated latent growth factor activation strategy was developed incorporating latent transforming growth factor-β1 (LTGF) into an electrospun poly(L-lactide) scaffold.
  
  
  METHODS
  The electrospun scaffold was surface modified with NH3 plasma and biofunctionalised with LTGF to produce both random and orientated biofunctionalised electrospun scaffolds. Scaffold surface chemical analysis and growth factor bioavailability assays were performed. In vitro biocompatibility and human nasal chondrocyte gene expression with these biofunctionalised electrospun scaffold templates were assessed. In vivo chondrogenic activity and chondrocyte gene expression were evaluated in athymic rats.
  
  
  RESULTS
  Chemical analysis demonstrated that LTGF anchored to the scaffolds was available for enzymatic, chemical and cell activation. The biofunctionalised scaffolds were non-toxic. Gene expression suggested chondrocyte re-differentiation after 14 days in culture. By 6 weeks, the implanted biofunctionalised scaffolds had induced highly passaged chondrocytes to re-express Col2A1 and produce type II collagen.
  
  
  CONCLUSIONS
  We have demonstrated a proof of concept for cell-mediated activation of anchored growth factors using a novel biofunctionalised scaffold in cartilage engineering. This presents a platform for development of protein delivery systems and for tissue engineering.
authors.affiliation: Department of Materials and Institute of Biomedical Engineering, Imperial College London, London, UK. ; The Blizard Institute of Cell and Molecular Science, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
authors.forename: Erh-Hsuin
authors.initials: EH
authors.lastname: Lim
authors.forename: Jose Paulo
authors.initials: JP
authors.lastname: Sardinha
authors.forename: Simon
authors.initials: S
authors.lastname: Myers
authors.forename: Molly
authors.initials: M
authors.lastname: Stevens
keywords: Biomimetics; Cartilage; Guided tissue regeneration; Tissue scaffolds; Transforming growth factor beta1