title: Phosphorus-31 nuclear magnetic resonance assignments of biogenic phosphorus compounds in sediment of an artificial Fuyangxin River, China.
abstract: River eutrophication could drastically influence the phosphorus (P) in the water and sediment. To understand the biogenic-P species, distribution and bioconversion, five sediment samples were collected from an artificial river, and analyzed by phosphorus-31 nuclear magnetic resonance ((31)P-NMR). The P pollution in the water and sediment were both severe. The average concentrations of total P (TP) and solution reactive phosphorus in the water were 3.0 and 2.6 mg L(-1), respectively, which surpass grade V of the national quality standard (China) and should not be used for any purpose. The river sediments accumulated significant inorganic phosphorus (Pi) and organic phosphorus (Po); in the P fractionation, the rank order of the P fractions was as follows: Ca-P > NaOH-Pi > Res-P > KCl-P > NaOH-Po, with average relative proportions of 25.1:16.8:6.6:1.7:1:0. Six P compounds were detected in the NaOH-EDTA extract by (31)P-NMR. Mono-P (8.96-29.58 %) was the dominant forms of biogenic-P, and other smaller fractions of biogenic-P were also observed, including pyro-P (0.22-0.86 %), DNA-P (0.75-2.03 %), phon-P (0-1.57 %), and lipids-P (0-2.66 %). The TP and biogenic-P decreased along the direction of flows, with their average relative proportions 7.97:1.20:1.49:1.00:1.00 and 40.87:2.34:3.46:1.60:1 from the upstream to downstream, respectively. The concentration and species of Po in NaOH-Po were lower than found in (31)P-NMR analysis in this research. Thus, the use of 0.25 M NaOH and 50 mM EDTA extracts and solution (31)P-NMR analysis was a more accurate method for quantifying biogenic-P in the river sediments than P fractionation.
authors.affiliation: State Key Laboratory on Environmental Aquatic Chemistry, Research Center for Eco-Environmental Science, Chinese Academy of Science, Beijing, 100085, China.
authors.forename: Wenqiang
authors.initials: W
authors.lastname: Zhang
authors.forename: Baoqing
authors.initials: B
authors.lastname: Shan
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Wenzhong
authors.initials: W
authors.lastname: Tang
mesh_terms: D005591:Chemical Fractionation; D002681:China; D004784:Environmental Monitoring / Q000379:methods; D019015:Geologic Sediments / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D017553:Phosphorus Compounds / Q000032:analysis*; D010759:Phosphorus Isotopes; D045483:Rivers / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D017553:Phosphorus Compounds; D010759:Phosphorus Isotopes; D014874:Water Pollutants, Chemical