title: SMG-ly knocking out gene expression in specific cells: an educational primer for use with "a novel strategy for cell-autonomous gene knockdown in caenorhabditis elegans defines a cell-specific function for the G-protein subunit GOA-1".
abstract: A recent article by Maher et al. in GENETICS introduces an alternative approach to cell-type-specific gene knockdown in Caenorhabditis elegans, using nonsense-mediated decay. This strategy has the potential to be applicable to other organisms (this strategy requires that animals can survive without nonsense-mediated decay-not all can). This Primer article provides a guide and resource for educators and students by describing different gene knockdown methodologies, by assisting with the technically difficult portions of the Maher et al. article, and by providing conceptual questions relating to the article.
authors.affiliation: Department of Biology, Haverford College, Haverford Pennsylvania 19041Department of Biology, Haverford College, Haverford Pennsylvania 19041.
authors.forename: Philip M
authors.initials: PM
authors.lastname: Meneely
authors.forename: Jordana C
authors.initials: JC
authors.lastname: Bloom
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000235:genetics / Q000378:metabolism; D055785:Gene Knockdown Techniques / Q000379:methods*; D005823:Genetics / Q000193:education*; D059365:Nonsense Mediated mRNA Decay
chemical_list: D029742:Caenorhabditis elegans Proteins; C516620:GOA-1 protein, C elegans; D019206:GTP-Binding Protein alpha Subunits, Gi-Go
keywords: cell-specific gene expression; education; nonsense-mediated decay (NMD)