title: Capacity for RNA synthesis in 70S ribosome-deficient plastids of heat-bleached rye leaves.
abstract: In the leaves of rye seedlings (Secale cereale L.) grown at an elevated temperature of 32°C the formation of plastidic 70S ribosomes is specifically prevented. The resulting plastid ribosome-deficient leaves, which are chlorotic in light, represent a system for the identification of translation products of the 80S ribosomes among the chloroplastic proteins. Searching for the primary heat-sensitive event causing the 70S ribosome-deficiency, the thermostability of the chloroplastic capacity for RNA synthesis was investigated. The RNA polymerase activity of isolated normal chloroplasts from 22°-grown rye leaves was not inactivated in vitro at temperatures between 30° and 40°C. The ribosome-deficient plastids purified from bleached 32°-grown leaf parts contained significant RNA polymerase activity which was, however, lower than in functional chloroplasts. After application of [(3)H]uridine to intact leaf tissues [(3)H]uridine incorporation was found in ribosome-deficient plastids of 32°C-grown leaves. The amount of incorporation was similar to that in the control chloroplasts from 22°C-grown leaves. According to these results, it is unlikely that the non-permissive temperature (32°C) causes a general inactivation of the chloroplastic RNA synthesis in rye leaves.
authors.affiliation: Fachbereich Biologie, Botanik, Johann-Wolfgang-Goethe-Universität, Siesmayerstraße 70, D-6000, Frankfurt/Main, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Bünger
authors.forename: J
authors.initials: J
authors.lastname: Feierabend