title: DNA-based nanosensors for next-generation clinical diagnostics via detection of enzyme activity.
abstract: Specific and sensitive detection of DNA-modifying enzymes represents a cornerstone in modern medical diagnostics. Many of the currently prevalent methods are not preferred in the clinics because they rely heavily on pre-amplification or post-separation steps. This editorial highlights the potential of adopting DNA-based nanosensors for the assessment of the activities of DNA-modifying enzymes, with emphasis on the topoisomerase and tyrosyl-DNA phosphodiesterase families. By underlining the existing challenges, we expect that the DNA-nanosensors may soon be promoted to clinical diagnostics via enzyme detection.
authors.affiliation: Department of Pathology, Aarhus University Hospital, Noerrebrogade 44, building 18B, 8000 Aarhus C, Denmark +45 7846 3672 magnstou@rm.dk.
authors.forename: Magnus
authors.initials: M
authors.lastname: Stougaard
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Ho
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use; D015374:Biosensing Techniques; D002166:Camptothecin / Q000494:pharmacology / Q000627:therapeutic use; D015342:DNA Probes; D026942:DNA Topoisomerases / Q000502:physiology; D057075:Enzyme Assays; D006801:Humans; D036103:Nanotechnology; D009369:Neoplasms / Q000188:drug therapy / Q000201:enzymology; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D010727:Phosphoric Diester Hydrolases / Q000502:physiology; D059003:Topoisomerase Inhibitors / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D015342:DNA Probes; D010726:Phosphodiesterase Inhibitors; D059003:Topoisomerase Inhibitors; D010727:Phosphoric Diester Hydrolases; C122147:tyrosyl-DNA phosphodiesterase; D026942:DNA Topoisomerases; D002166:Camptothecin