title: Expression of bovine Mx1 protein inhibits the replication of foot-and-mouth disease virus in BHK-21 cells.
abstract: Mx proteins belonging to the dynamin superfamily of large GTPases inhibit replication of a wide range of RNA viruses. In this study, we examined whether bovine Mx1 protein could interfere with the replication of foot-and-mouth disease virus (FMDV). For this purpose we established cloned BHK-21 cells expressing bovine Mx1 protein (BM1 cells) and infected them with FMDV serotype O. Cloned BHK-21 cells expressing neomycin resistance instead of Mx1 protein (BH1 cells) and original BHK-21 cells served as negative controls. The results showed that the expression of bovine Mx1 protein reduced viral yields by 90% and levels of viral VP1 mRNA by 60%. These findings correlated with a significant reduction of viral antigen detectable in infected cells by immunofluorescent assay. These results demonstrate that bovine Mx1 protein interferes with the replication of FMDV.
authors.forename: K J
authors.initials: KJ
authors.lastname: Cai
authors.forename: Q L
authors.initials: QL
authors.lastname: Meng
authors.forename: J
authors.initials: J
authors.lastname: Qiao
authors.forename: J
authors.initials: J
authors.lastname: Huang
authors.forename: Z C
authors.initials: ZC
authors.lastname: Zhang
authors.forename: G C
authors.initials: GC
authors.lastname: Wang
authors.forename: J W
authors.initials: JW
authors.lastname: Wang
authors.forename: C F
authors.initials: CF
authors.lastname: Chen
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D002460:Cell Line; D006224:Cricetinae; D015536:Down-Regulation; D005536:Foot-and-Mouth Disease / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000502:physiology*; D015870:Gene Expression; D064547:Myxovirus Resistance Proteins / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication
chemical_list: D064547:Myxovirus Resistance Proteins