title: Tetra-repeat microsatellite markers for the masu salmon (Oncorhynchus masou masou) and its application in cross-subspecies amplification.
abstract: We developed tetranucleotide-repeat microsatellite markers for the masu salmon (Oncorhynchus masou) complex. 454 pyrosequencing was used to discover repeat motifs, and seven polymorphic microsatellite-primer sets were identified. The number of alleles detected at each locus ranged from four to 24 and the expected heterozygosity varied from 0.57 to 0.92. Cross-subspecies amplification for O. m. masou, O. m. ishikawae and O. m. subsp. was successful. These microsatellites can be utilized in studies of genetic structure, genetic diversity, and intra- and inter-subspecific hybridization, making a contribution to conservation and management of the Oncorhynchus masou complex.
authors.affiliation: National Research Institute of Aquaculture, Fisheries Research Agency, Nikko, Tochigi 321-1661, Japan. ysho@affrc.go.jp.
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Tadahide
authors.initials: T
authors.lastname: Kurokawa
authors.forename: Masashi
authors.initials: M
authors.lastname: Sekino
authors.forename: Motoshige
authors.initials: M
authors.lastname: Yasuike
authors.forename: Kenji
authors.initials: K
authors.lastname: Saitoh
mesh_terms: D000483:Alleles; D000818:Animals; D003247:Conservation of Natural Resources; D005828:Genetics, Population; D018895:Microsatellite Repeats / Q000235:genetics*; D017683:Oncorhynchus / Q000235:genetics* / Q000254:growth & development