title: Evaluation of p16(INK4a) overexpression in a large series of cervical carcinomas: concordance with SPF10-LiPA25 PCR.
abstract: The aims of this study were to assess the overexpression of p16 in formalin-fixed paraffin-embedded cervical carcinoma tissue blocks and to determine its concordance with the human papillomavirus (HPV) status using the SPF10-LiPA25 polymerase chain reaction System and its correlation with the histologic type of invasive cervical cancer (ICC) and individual HPV genotypes. A total of 205 retrospectively collected ICC cases were analyzed by p16 immunohistochemistry. HPV detection was performed by polymerase chain reaction using SPF10 broad-spectrum primers, followed by deoxyribonucleic acid enzyme immunoassay and genotyping by reverse hybridization line probe assay (LiPA25). Of 205, 188 analyzed (91.7%) ICC cases showed p16 overexpression, whereas 181 (83.3%) cases were HPV positive using HPV LiPA testing. One hundred and seventy four (84.9%) cases were both p16 and HPV LiPA positive, indicating a positive concordance of 89.9% between both techniques (κ index agreement of 0.43; P<0.001), and no statistically significant difference (McNemar test, P>0.05). Squamous cell carcinomas were strongly positive compared with the adenocarcinomas (93.6% vs. 75% of the cases, respectively). When performed on formalin-fixed paraffin-embedded cervical tissue specimens, the higher positivity rate of p16 immunohistochemistry as compared with HPV DNA testing may allow identifying HPV-related ICC cases in which HPV testing was negative.
authors.affiliation: Department of Pathology (C.P., J.V.), San Agustín Hospital, University Institute of Oncology of the Principality of Asturias (IUOPA-HUCA) Gynaecology Department (M.C.), Jarrio Hospital, Coaña, SESPA, Asturias Unit of Infections and Cancer, Cancer Epidemiology Research Programme (L.A., S.T., J.K., S.S.), IDIBELL, Catalan Institute of Oncology (ICO), L'Hospitalet de Llobregat CIBER Epidemiology and Public Health (CIBERESP) (S.S.), Institut Catalan Institute of Oncology (ICO), L'Hospitalet de Llobregat, Barcelona, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Pérez
authors.forename: Marta
authors.initials: M
authors.lastname: Castillo
authors.forename: Laia
authors.initials: L
authors.lastname: Alemany
authors.forename: Sara
authors.initials: S
authors.lastname: Tous
authors.forename: Joellen
authors.initials: J
authors.lastname: Klaustermeier
authors.forename: Silvia
authors.initials: S
authors.lastname: de Sanjose
authors.forename: Julio
authors.initials: J
authors.lastname: Velasco
mesh_terms: D002277:Carcinoma / Q000378:metabolism / Q000821:virology*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D030361:Papillomavirus Infections / Q000175:diagnosis*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000821:virology*
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D004279:DNA, Viral