title: Histological study on effect of mesenchymal stem cell therapy on experimental renal injury induced by ischemia/reperfusion in male albino rat.
abstract: OBJECTIVE
  Acute kidney injury (AKI) represents a major clinical problem with high mortality and limited treatment protocols. This study was planned to evaluate the therapeutic effectiveness of bone marrow - derived mesenchymal stem cells (BM-MSCs) in a rat model of ischemia/reperfusion (I/R) AKI.
  
  
  RESULTS
  This study was carried out on thirty adult male albino rats. Animals were divided equally into three groups. Group I (control sham-operated group) (n=10), were subdivided equally into two subgroups; Ia and Ib. The experimental group (n=20) were all subjected to I/R injury by clamping both renal pedicles for 40 minutes. Half of the I/R animals did not receive MSC therapy (group II) [non-MSC treated group]. The other half of the I/R animals received single intravenous injection of PKH26 labelled BM-MSCs immediately after removal of the clamps and visual confirmation of reflow (group III) [MSC treated group]. Animals were sacrificed 24 hrs (subgroups IIa & IIIa) and 72 hrs (subgroups IIb & IIIb) after intervention. Serological measurements included serum urea and creatinine. Kidney specimens were processed for H&E, PAS and PCNA. Mean % of renal corpuscles with affected glomeruli, mean % of affected tubules, mean area % of PAS-positive reaction and mean area % of PCNA immunoreactivity were measured by histomorphometric studies and statistically compared. MSCs-treated group exhibited protection against renal injury serologically and histologically.
  
  
  CONCLUSIONS
  Results of the present study suggest a potential reno-protective capacity of MSCs which could be of considerable therapeutic promise for cell-based management of clinical AKI.
authors.affiliation: Department of Histology, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Eman Mostafa
authors.initials: EM
authors.lastname: Sadek
authors.forename: Noha Mohamed
authors.initials: NM
authors.lastname: Afifi
authors.forename: Lamiaa Ibrahim Abd
authors.initials: LI
authors.lastname: Elfattah
authors.forename: Manal Ali Abd-El
authors.initials: MA
authors.lastname: Mohsen
keywords: Acute kidney injury; Ischaemia reperfusion; Mesenchymal stem cells; Proliferating cell nuclear antigen