title: Matrix metalloproteinase-2 (-1306 c>t) promoter polymorphism and risk of colorectal cancer in the Saudi population.
abstract: BACKGROUND
  Matrix metalloproteinase-2 (MMP-2) is an enzyme with proteolytic activity against matrix proteins, particularly basement membrane constituents. A single nucleotide polymorphism (SNP) at -1306, which disrupts a Sp1-type promoter site (CCACC box), results in strikingly lower promoter activity with the T allele. In the present study, we investigated whether this MMP-2 genetic polymorphism might be associated with susceptibility to colorectal cancer (CRC) in the Saudi population. We also analyzed MMP-2 gene expression level sin CRC patients and 4 different cancer cell lines.
  
  
  METHODS
  TaqMan allele discrimination assays and DNA sequencing techniques were used to investigate the C-1306T SNP in the MMP-2 gene of Saudi colorectal cancer patients and controls. The MMP-2 gene expression level was also determined in 12 colon cancer tissue samples collected from unrelated patients and histologically normal tissues distant from tumor margins.
  
  
  CONCLUSIONS
  The MMP-2 C-1306T SNP in the promoter region was associated with CRC in our Saudi population and the MMP-2 gene expression level was found to be 10 times higher in CRC patients. The MMP-2 C-1306T SNP is significantly associated with CRC in the Saudi population and this finding suggested that MMP-2 variants might help predict CRC progression risk among Saudis. We propose that analysis of this gene polymorphism could assist in identification of patient subgroups at risk of a poor disease outcome.
authors.affiliation: Biochemistry Department, College of Science, King Saud University, Riyadh, KSA E-mail : hesham25166@yahoo.com.
authors.forename: Hesham Mahmoud
authors.initials: HM
authors.lastname: Saeed
authors.forename: Mohammad Saud
authors.initials: MS
authors.lastname: Alanazi
authors.forename: Narasimha Reddy
authors.initials: NR
authors.lastname: Parine
authors.forename: Jilani
authors.initials: J
authors.lastname: Shaik
authors.forename: Abdelhabib
authors.initials: A
authors.lastname: Semlali
authors.forename: Othman
authors.initials: O
authors.lastname: Alharbi
authors.forename: Nahla
authors.initials: N
authors.lastname: Azzam
authors.forename: Abdulrahman
authors.initials: A
authors.lastname: Aljebreen
authors.forename: Majid
authors.initials: M
authors.lastname: Almadi
authors.forename: Manal Aly
authors.initials: MA
authors.lastname: Shalaby
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000453:epidemiology / Q000209:etiology*; D004247:DNA / Q000032:analysis / Q000235:genetics; D005260:Female; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012529:Saudi Arabia / Q000453:epidemiology; D014407:Tumor Cells, Cultured
chemical_list: D004247:DNA; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2