title: RON (recepteur d'origine nantais) expression and its association with tumor progression in laryngeal squamous cell carcinoma.
abstract: OBJECTIVE
  Several human tumor tissues show an aberrant expression and activation of recepteur d'origine nantais (RON). In this paper, we investigate the expression of RON in human laryngeal squamous cell carcinoma (SCC) and evaluate whether RON affects the tumor cell progression in human laryngeal SCC cell line.
  
  
  METHODS
  Immunohistochemistry was used to assess RON expression in human laryngeal SCC. To evaluate the impact of RON knockdown, the cell invasion assay and cell migration assay using small-interfering RNA were performed.
  
  
  RESULTS
  The expression of RON protein was dominantly observed in laryngeal SCC tissues relative to adjacent normal mucosa in all cases. Knockdown of RON resulted in significantly reduced cell invasion in human laryngeal SCC cells. Cell migration showed a marked decrease in RON knockdown laryngeal SCC cells compared to the negative control laryngeal SCC cells. Laryngeal SCC cell migration was enhanced by incubation with macrophage stimulating protein (MSP).
  
  
  CONCLUSIONS
  RON is highly expressed in human laryngeal SCC. We suggest that RON plays an important role in invasion, and metastasis of laryngeal SCC.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Chonnam National University Medical School, Research Institute of Medical Sciences, Chonnam National University and Hwasun Hospital, Hwasun, South Korea.
authors.forename: Sun-Ae
authors.initials: SA
authors.lastname: Kim
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Chonnam National University Medical School, Research Institute of Medical Sciences, Chonnam National University and Hwasun Hospital, Hwasun, South Korea.
authors.forename: Tae Mi
authors.initials: TM
authors.lastname: Yoon
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Chonnam National University Medical School, Research Institute of Medical Sciences, Chonnam National University and Hwasun Hospital, Hwasun, South Korea.
authors.forename: Dong Hoon
authors.initials: DH
authors.lastname: Lee
authors.affiliation: Department of Internal Medicine, Chonnam National University Medical School, Research Institute of Medical Sciences, Chonnam National University and Hwasun Hospital, Hwasun, South Korea.
authors.forename: Young-Lan
authors.initials: YL
authors.lastname: Park
authors.affiliation: Department of Pathology, Chonnam National University Medical School, Research Institute of Medical Sciences, Chonnam National University and Hwasun Hospital, Hwasun, South Korea.
authors.forename: Kyung-Hwa
authors.initials: KH
authors.lastname: Lee
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Chonnam National University Medical School, Research Institute of Medical Sciences, Chonnam National University and Hwasun Hospital, Hwasun, South Korea.
authors.forename: Sang Chul
authors.initials: SC
authors.lastname: Lim
authors.affiliation: Department of Internal Medicine, Chonnam National University Medical School, Research Institute of Medical Sciences, Chonnam National University and Hwasun Hospital, Hwasun, South Korea.
authors.forename: Young-Eun
authors.initials: YE
authors.lastname: Joo
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Chonnam National University Medical School, Research Institute of Medical Sciences, Chonnam National University and Hwasun Hospital, Hwasun, South Korea. Electronic address: joonkyoo@chonnam.ac.kr.
authors.forename: Joon Kyoo
authors.initials: JK
authors.lastname: Lee
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D054443:Cell Migration Assays; D002465:Cell Movement / Q000235:genetics; D018450:Disease Progression; D005260:Female; D055785:Gene Knockdown Techniques; D006258:Head and Neck Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics; D034741:RNA, Small Interfering; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D034741:RNA, Small Interfering; C080835:RON protein; D020794:Receptor Protein-Tyrosine Kinases
keywords: Disease progression; Laryngeal cancer; Metastasis; Molecular targeted therapy; RON protein