title: Transcriptional profiling of genes involved in n-hexadecane compounds assimilation in the hydrocarbon degrading Dietzia cinnamea P4 strain.
abstract: The petroleum-derived degrading Dietzia cinnamea strain P4 recently had its genome sequenced and annotated. This allowed employing the data on genes that are involved in the degradation of n-alkanes. To examine the physiological behavior of strain P4 in the presence of n-alkanes, the strain was grown under varying conditions of pH and temperature. D. cinnamea P4 was able to grow at pH 7.0-9.0 and at temperatures ranging from 35 ºC to 45 ºC. Experiments of gene expression by real-time quantitative RT-PCR throughout the complete growth cycle clearly indicated the induction of the regulatory gene alkU (TetR family) during early growth. During the logarithmic phase, a large increase in transcriptional levels of a lipid transporter gene was noted. Also, the expression of a gene that encodes the protein fused rubredoxin-alkane monooxygenase was enhanced. Both genes are probably under the influence of the AlkU regulator.
authors.affiliation: Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Ilha do Fundão, Rio de Janeiro, RJ, Brazil. ; Department of Microbial Ecology, University of Groningen, Kerklaan, Haren, The Netherlands.
authors.forename: Luciano
authors.initials: L
authors.lastname: Procópio
authors.forename: Michele
authors.initials: M
authors.lastname: de Cassia Pereira e Silva
authors.forename: Jan Dirk
authors.initials: JD
authors.lastname: van Elsas
authors.forename: Lucy
authors.initials: L
authors.lastname: Seldin
mesh_terms: D000192:Actinomycetales / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D000473:Alkanes / Q000378:metabolism*; D001711:Biotransformation; D020869:Gene Expression Profiling; D005798:Genes, Bacterial; D006838:Hydrocarbons / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D013696:Temperature
chemical_list: D000473:Alkanes; D006838:Hydrocarbons; C007932:n-hexadecane
keywords: Dietzia cinnamea; alk genes; n-hexadecane assimilation; real-time quantitative RT-PCR; rubredoxin-alkane monooxygenase