title: DNA sequence organization in the genomes of three related millet plant species.
abstract: A major portion of the genomes of three millet species, namely, barn yard millet, fox tail millet and little millet has been shown to consist of interspersed repeat and single copy DNA sequences. The interspersed repetitive DNA sequences are both short (0.15-1.0 kilo base pairs, 62-64% and long (>1.5 kilo base pairs, 36-38%) in barn yard millet and little millet while in fox tail millet, only long interspersed repeats (>1.5 kilo base pairs) are present. The length of the interspersed single copy DNA sequences varies in the range of 1.6-2.6 kilo base pairs in all the three species. The repetitive duplexes isolated after renaturation of 1.5 kilo base pairs and 20 kilo base pairs long DNA fragments exhibit a high thermal stability with Tms either equal to or greater than the corresponding native DNAs. The S1 nuclease resistant repetitive DNA duplexes also are thermally stable and reveal the presence of only 1-2% sequence divergence.The present data on the modes of sequence arrangement in millets substantiates the proposed trend in plants, namely, plants with 1C nuclear DNA content of less than 5 picograms have diverse patterns of sequence organization while those with 1C nuclear DNA content greater than 5 picograms have predominantly a short period interspersion pattern.
authors.affiliation: Biochemistry Division, National Chemical Laboratory, Pune, 411 008, India.
authors.forename: L
authors.initials: L
authors.lastname: Sivaraman
authors.forename: V S
authors.initials: VS
authors.lastname: Gupta
authors.forename: P K
authors.initials: PK
authors.lastname: Ranjekar