title: Ultrastructural changes in the endoplasmic reticulum during dormancy release of apple embryos (Pyrus malus L.).
abstract: Apple embryos were treated by cold (0°C) within the fruits, to break their dormancy; the controls were treated at 12°C or at 20°C. Ultrastructural features of meristematic cells in the embryonic axis were compared for each treatment. The organization of the cells of dormant embryos was described: Endoplasmic reticulum consisted in some short rough cisternae; lipid droplets regularly arranged near the plasmalemma constituted a kind of shell; mitochondria had a few cristae; and dictyosomes were rarely observed. All these features are typical of dry seeds. After cold treatments, the only evolution observed was in the endoplasmic reticulum, where highly organized stacks appeared progressively as a function of time at 0°C. An intermediate temperature (12°C) induced similar formations in the reticulum but they were rarely observed and their degree of organization was lower than that obtained at 0°C. At 20°C, endoplasmic reticulum resembled that of the dormant embryo cells. The relation between the appearance of these structures in the reticulum and the disappearance of dormancy induced by cold is discussed.
authors.affiliation: Laboratoire de Physiologie Végétale Appliquée, Université Pierre et Marie Curie (Paris VI), Tour 53, 4 Place Jussieu, F-75230, Paris Cedex 05, France.
authors.forename: M
authors.initials: M
authors.lastname: Bouvier-Durand
authors.forename: J
authors.initials: J
authors.lastname: Dereuddre
authors.forename: D
authors.initials: D
authors.lastname: Côme