title: And the winner is...azadithiolate: an amine proton relay in the [FeFe] hydrogenases.
abstract: A victory in the pocket: An international team of chemists and biophysicists have resolved the long-standing question of the structure of the active site of the [FeFe] hydrogenases by assembling the active enzyme with a version of the active site synthesized in vitro (see scheme; HydF is a scaffold protein, HydA1 is a natural hydrogenase). The protein incorporating the diiron complex 2 showed similar activity to that of the natural enzyme.
authors.affiliation: Department of Chemistry, University of Illinois, 600 South Mathews Avenue, M/C 712, Urbana (USA) http://www.scs.illinois.edu/rauchfus/. schilter@illinois.edu.
authors.forename: David
authors.initials: D
authors.lastname: Schilter
authors.forename: Thomas B
authors.initials: TB
authors.lastname: Rauchfuss
keywords: cofactors; enzymology; hydrogen; hydrogenases; iron