title: Mass production of early-stage bone-marrow-derived mesenchymal stem cells of rat using gelatin-coated matrix.
abstract: Although preparation of early-stage bone-marrow-derived mesenchymal stem cells (BM-MSCs) is critical for successful cell transplantation therapy, no culture system offers a sufficient number of early-stage BM-MSCs for cell transplantation. Accordingly, we developed a culture system capable of producing a large number of early-stage BM-MSCs by using gelatin-coated matrix. The greatest retrieval and proliferation rates of the earliest-stage rat BM-MSCs were detected in bone-marrow-derived cells cultured on 1% (wt/v) gelatin-coated matrix, which showed significantly greater colony forming unit-fibroblast number, diameter, and total cell number. Moreover, continuous culture of the earliest-stage BM-MSCs on 1% (wt/v) gelatin-coated matrix resulted in a maximum of 21.2 ± 2.7 fold increase in the cumulative total number of early-stage BM-MSCs at passage 5. BM-MSCs generated in large quantities due to a reduced doubling time and an increased yield of cell population in S/G2/M phase showed typical fibroblast-like morphology and no significant differences in BM-MSC-related surface marker expression and differentiation potential, except for an increased ratio of differentiation into a neurogenic lineage. The use of gelatin-coated matrix in the retrieval and culture of BM-MSCs contributes greatly to the effective isolation and mass production of early-stage BM-MSCs.
authors.affiliation: Stem Cell and Bioevaluation, WCU Biomodulation Program, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Young Hyun
authors.initials: YH
authors.lastname: Park
authors.forename: Jung Im
authors.initials: JI
authors.lastname: Yun
authors.forename: Na Rae
authors.initials: NR
authors.lastname: Han
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Park
authors.forename: Ji Yeon
authors.initials: JY
authors.lastname: Ahn
authors.forename: Choonghyo
authors.initials: C
authors.lastname: Kim
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Choi
authors.forename: Eunsong
authors.initials: E
authors.lastname: Lee
authors.forename: Jeong Mook
authors.initials: JM
authors.lastname: Lim
authors.forename: Seung Tae
authors.initials: ST
authors.lastname: Lee
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation; D017690:Cell Transplantation; D064987:Cell- and Tissue-Based Therapy; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051381:Rats
chemical_list: D005780:Gelatin