title: Astragalus Polysaccharide Suppresses the Expression of Adhesion Molecules through the Regulation of the p38 MAPK Signaling Pathway in Human Cardiac Microvascular Endothelial Cells after Ischemia-Reperfusion Injury.
abstract: Astragalus polysaccharide is a major component of radix astragali, a vital qi-reinforcing herb medicine with favorable immune-regulating effects. In a previous animal experiment, we demonstrated that astragalus polysaccharide effectively alleviates ischemia-reperfusion injury (IRI) of cardiac muscle through the regulation of the inflammatory reactions. However, the relationship between this herb and the cohesion molecules on the cell surface remains controversial. In this study, human cardiac microvascular endothelial cells (HCMECs) were used to validate the protective effects of astragalus under an IRI scheme simulated through hypoxia/reoxygenation in vitro. The results indicated that astragalus polysaccharide inhibited the cohesion between HCMECs and polymorphonuclear leukocyte (PMN) during IRI through the downregulation of p38 MAPK signaling and the reduction of cohesive molecule expression in HCMECs.
authors.affiliation: Institute for Cardiovascular Disease, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100700, China ; Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100700, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Hai-Yan
authors.forename: Gao
authors.initials: G
authors.lastname: Yong-Hong
authors.forename: Wang
authors.initials: W
authors.lastname: Zhi-Yao
authors.forename: Xu
authors.initials: X
authors.lastname: Bing
authors.forename: Wu
authors.initials: W
authors.lastname: Ai-Ming
authors.forename: Xing
authors.initials: X
authors.lastname: Yan-Wei
authors.forename: Liu
authors.initials: L
authors.lastname: Bei
authors.forename: Lou
authors.initials: L
authors.lastname: Li-Xia
authors.forename: Chen
authors.initials: C
authors.lastname: Li-Xin