title: Phenotypic and functional properties of feline dedifferentiated fat cells and adipose-derived stem cells.
abstract: It has been reported that mature adipocyte-derived dedifferentiated fat (DFAT) cells show multilineage differentiation potential similar to that observed in mesenchymal stem cells. Since DFAT cells can be prepared from a small quantity of adipose tissue, they could facilitate cell-based therapies in small companion animals such as cats. The present study examined whether multipotent DFAT cells can be generated from feline adipose tissue, and the properties of DFAT cells were compared with those of adipose-derived stem cells (ASCs). DFAT cells and ASCs were prepared from the floating mature adipocyte fraction and the stromal vascular fraction, respectively, of collagenase-digested feline omental adipose tissue. Both cell types were evaluated for growth kinetics, colony-forming unit fibroblast (CFU-F) frequency, immunophenotypic properties, and multilineage differentiation potential. DFAT cells and ASCs could be generated from approximately 1g of adipose tissue and were grown and subcultured on laminin-coated dishes. The frequency of CFU-Fs in DFAT cells (35.8%) was significantly higher than that in ASCs (20.8%) at passage 1 (P1). DFAT cells and ASCs displayed similar immunophenotypes (CD44(+), CD90(+), CD105(+), CD14(-), CD34(-) and CD45(-)). Alpha-smooth muscle actin-positive cells were readily detected in ASCs (15.2±7.2%) but were rare in DFAT cells (2.2±3.2%) at P1. Both cell types exhibited adipogenic, osteogenic, chondrogenic, and smooth muscle cell differentiation potential in vitro. In conclusion, feline DFAT cells exhibited similar properties to ASCs but displayed higher CFU-F frequency and greater homogeneity. DFAT cells, like ASCs, may be an attractive source for cell-based therapies in cats.
authors.affiliation: Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, College of Bioresource Science, Nihon University, Fujisawa 252-0880, Japan.
authors.forename: Shota
authors.initials: S
authors.lastname: Kono
authors.affiliation: Department of Functional Morphology, Division of Cell Regeneration and Transplantation, Nihon University School of Medicine, Tokyo 173-8610, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Kazama
authors.affiliation: Laboratory of Cell and Tissue Biology, College of Bioresource Science, Nihon University, Fujisawa 252-0880, Japan.
authors.forename: Koichiro
authors.initials: K
authors.lastname: Kano
authors.affiliation: Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, College of Bioresource Science, Nihon University, Fujisawa 252-0880, Japan.
authors.forename: Kayoko
authors.initials: K
authors.lastname: Harada
authors.affiliation: Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, College of Bioresource Science, Nihon University, Fujisawa 252-0880, Japan.
authors.forename: Masami
authors.initials: M
authors.lastname: Uechi
authors.affiliation: Department of Functional Morphology, Division of Cell Regeneration and Transplantation, Nihon University School of Medicine, Tokyo 173-8610, Japan. Electronic address: matsumoto.taro@nihon-u.ac.jp.
authors.forename: Taro
authors.initials: T
authors.lastname: Matsumoto
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000502:physiology; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002415:Cats; D018929:Cell Culture Techniques; D054337:Cell Dedifferentiation; D005260:Female; D010012:Osteogenesis; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology; D054457:Tissue Scaffolds
keywords: Adipose-derived stem cell; Feline; Mature adipocyte; Mesenchymal stem cell; Regenerative medicine