title: Effects of flavonoid-containing preparation Extralife on hydrogen peroxide production and functioning of mitochondrial ATP-dependent potassium channel.
abstract: Extralife, a Pentaphylloides fruticos extract, in concentrations of 0.005-10 μg/ml dose-dependently increased H2O2 production in rat heart mitochondria in the presence of respiration substrates. Extralife decreased ATP-induced accumulation of H2O2 related to inhibition of mitochondrial ATP-dependent potassium channel. This effect was observed only at low doses of the adaptogen (0.05-3 μg/ml). High doses of the substance (5-10 μg/ml) did not abolish ATP-dependent production of H2O2 and increased the rate of H2O2 generation by the mitochondria. We concluded that Extralife in trace concentrations could activate mitochondrial ATP-dependent potassium channel and decrease H2O2 accumulation in the mitochondria.
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences; Pushchino State Institute of Natural Sciences, Moscow Region; Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russia. ldlukyanova@gmail.com.
authors.forename: S V
authors.initials: SV
authors.lastname: Murzaeva
authors.forename: S P
authors.initials: SP
authors.lastname: Belova
authors.forename: E I
authors.initials: EI
authors.lastname: Lezhnev
authors.forename: L D
authors.initials: LD
authors.lastname: Luk'yanova
authors.forename: G D
authors.initials: GD
authors.lastname: Mironova
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology / Q000502:physiology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D003652:Decanoic Acids / Q000494:pharmacology; D018698:Glutamic Acid / Q000494:pharmacology / Q000502:physiology; D006861:Hydrogen Peroxide / Q000378:metabolism*; D006880:Hydroxy Acids / Q000494:pharmacology; D054086:KATP Channels / Q000378:metabolism*; D008293:Malates / Q000494:pharmacology; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D026902:Potassium Channel Blockers / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012402:Rotenone / Q000494:pharmacology; D019802:Succinic Acid / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D003652:Decanoic Acids; D006880:Hydroxy Acids; D054086:KATP Channels; D008293:Malates; D010936:Plant Extracts; D026902:Potassium Channel Blockers; C527119:extralife; D012402:Rotenone; D018698:Glutamic Acid; C052853:5-hydroxydecanoic acid; C030298:malic acid; D000255:Adenosine Triphosphate; D019802:Succinic Acid; D006861:Hydrogen Peroxide