title: Ultraviolet photochemical cross-linking to detect RNA-binding proteins.
abstract: RNA is photoreactive on exposure to ultraviolet (UV) light in the 250- to 270-nm range. On UV treatment, RNA bases absorb energy to generate free radicals that can covalently attach to nearby amino acid residues in RNA-bound proteins. UV cross-linking experiments have been extensively used to identify and characterize RNA-binding proteins. The method described here involves the use of (32)P-labeled RNA and crude extracts or purified proteins.
authors.forename: Donald C
authors.initials: DC
authors.lastname: Rio
mesh_terms: D003432:Cross-Linking Reagents / Q000378:metabolism*; D007553:Isotope Labeling; D010761:Phosphorus Radioisotopes / Q000378:metabolism; D012313:RNA / Q000378:metabolism* / Q000528:radiation effects*; D016601:RNA-Binding Proteins / Q000032:analysis* / Q000302:isolation & purification*; D014466:Ultraviolet Rays
chemical_list: D003432:Cross-Linking Reagents; D010761:Phosphorus Radioisotopes; D016601:RNA-Binding Proteins; D012313:RNA