title: DNA methylation analysis of the gene CDKN2B in Gallus gallus (chicken).
abstract: Methylation is an epigenetic modification of DNA affecting gene expression without changing the structure of nucleotides. It plays a crucial role in the embryonic and post-embryonic development of living organisms. Methylation level is tissue and species-specific and changes with age. The study was aimed at identifying the methylation of the CDKN2B gene situated at locus bar in Polbar chickens on the 6th and 18th day of embryonic development using the MSP (methylation-specific PCR) method. Methylation was not detected in the promoter region of gene CDKN2B on the 6th and 18th day of embryonic development. As one of the five genes responsible for melanine activity in melanocytes and highly active, it can contribute to the production of this pigment. The present research broadens the current knowledge of the chicken epigenome and the mechanism of autosexing in birds.
authors.affiliation: Department of Biological Basis of Animal Production, University of Life Sciences, Akademicka 13, 20-950 Lublin, Poland. Magdalena.gryzinska@up.lublin.pl
authors.forename: Magdalena
authors.initials: M
authors.lastname: Gryzińska
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Andraszek
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Jocek
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics*; D050762:Cyclin-Dependent Kinase Inhibitor p15 / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation; D005260:Female; D014644:Genetic Variation; D008297:Male; D010860:Pigments, Biological
chemical_list: D050762:Cyclin-Dependent Kinase Inhibitor p15; D010860:Pigments, Biological