title: Increased 26S proteasome non-ATPase regulatory subunit 1 in the aqueous humor of patients with age-related macular degeneration.
abstract: Age-related macular degeneration (AMD) is the leading cause of blindness in the world. Evidence indicates that the suppression of the ubiquitin-proteasome system (UPS) contributes to the accumulation of toxic proteins and inflammation in retinal pigment epithelium (RPE), the functional abnormalities and/or the degeneration of which are believed to be the initiators and major pathologies of AMD. To identify new protein associations with the altered UPS in AMD, we used LC-ESI-MS/MS to perform a proteomic analysis of the aqueous humor (AH) of AMD patients and matched control subjects. Six UPS-related proteins were present in the AH of the patients and control subjects. Four of the proteins, including 26S proteasome non-ATPase regulatory subunit 1 (Rpn2), were increased in patients, according to semi-quantitative proteomic profiling. An LC-MRM assay revealed a significant increase of Rpn2 in 15 AMD patients compared to the control subjects, suggesting that this protein could be a biomarker for AMD.
authors.affiliation: Department of Ophthalmology, Konkuk University School of Medicine, Seoul 143-729, Korea.
authors.forename: Hyungwoo
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Ophthalmology, Konkuk University School of Medicine, Seoul 143-729, Korea.
authors.forename: Ae Jin
authors.initials: AJ
authors.lastname: Choi
authors.affiliation: Diatech Korea Co., Ltd, Seoul 138-826, Korea.
authors.forename: Gum-Yong
authors.initials: GY
authors.lastname: Kang
authors.affiliation: Diatech Korea Co., Ltd, Seoul 138-826, Korea.
authors.forename: Hyung Soon
authors.initials: HS
authors.lastname: Park
authors.affiliation: Department of Ophthalmology, Konkuk University School of Medicine, Seoul 143-729, Korea.
authors.forename: Hyung Chan
authors.initials: HC
authors.lastname: Kim
authors.affiliation: Department of Biomedical Science & Technology, Konkuk University, Seoul 143-729, Korea.
authors.forename: Hyunjung Jade
authors.initials: HJ
authors.lastname: Lim
authors.affiliation: Department of Ophthalmology, Konkuk University School of Medicine, Seoul 143-729, Korea.
authors.forename: Hyewon
authors.initials: H
authors.lastname: Chung
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001082:Aqueous Humor / Q000201:enzymology*; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D016022:Case-Control Studies; D005260:Female; D006602:Hexosyltransferases; D006801:Humans; D008268:Macular Degeneration / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D040901:Proteomics; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D015415:Biomarkers; C484162:PSMD1 protein, human; D006602:Hexosyltransferases; C517590:RPN2 protein, human; D046988:Proteasome Endopeptidase Complex