title: Neuroprotective effect of Chunghyuldan (Qing Xue Dan) on hypoxia-reoxygenation induced damage of neuroblastoma 2a cell lines.
abstract: OBJECTIVE
  Chunghyuldan (CHD), a combinatorial drug that has anti-hyperlipidemic and antiinflammatory activities, has been shown to reduce infarct volume in a focal ischemia-reperfusion rat model. To explore the molecular basis of CHD's neuroprotective effect, we examined whether CHD shows a cell-protective activity and has a regulatory effect on Bax and/or B-cell leukemia/lymphoma 2 (Bcl-2) expression in mouse neuroblastoma 2a (N2a) cells subjected to hypoxia-reoxygenation (H/R).
  
  
  METHODS
  In order to evaluate the effects of CHD on the cytotoxicity induced from hypoxia or H/R condition, lactate dehydrogenase (LDH) assay was performed. To explore whether the suppression of neural damage when pre-treated with CHD is associated with its anti-apoptotic effect, the CHD effect on the expression of Bcl-2 and Bax was analyzed by Western blotting analysis.
  
  
  RESULTS
  Cytotoxicity of N2a cell line was slightly increased in 42 h hypoxia condition and dramatically increased under the H/R condition. CHD treatment markedly decreased the cytotoxicity in both conditions (P<0.01, P<0.05). H/R markedly increased the expression of the pro-apoptotic protein, Bax, but slightly increased the expression of the anti-apoptotic protein, Bcl-2, compared with the normoxia or hypoxia group. CHD significantly decreased Bax expression (P<0.01) and slightly decreased Bcl-2 expression (P>0.05), resulted in a reduction of Bax/Bcl-2 ratio in N2a cells subjected to H/R.
  
  
  CONCLUSIONS
  CHD has neuroprotective effect in N2a cells subjected to H/R, which might be derived at least in part from its ability to decrease the expression of the pro-apoptotic protein, Bax.
authors.affiliation: Department of Cardiology and Neurology Diseases, College of Korean Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: Chang-Nam
authors.initials: CN
authors.lastname: Ko
authors.forename: In-Seo
authors.initials: IS
authors.lastname: Park
authors.forename: Seong-Uk
authors.initials: SU
authors.lastname: Park
authors.forename: Woo-Sang
authors.initials: WS
authors.lastname: Jung
authors.forename: Sang-Kwan
authors.initials: SK
authors.lastname: Moon
authors.forename: Jung-Mi
authors.initials: JM
authors.lastname: Park
authors.forename: Chulhun
authors.initials: C
authors.lastname: Kang
authors.forename: Ki-Ho
authors.initials: KH
authors.lastname: Cho
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D000860:Hypoxia / Q000517:prevention & control*; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D015427:Reperfusion Injury / Q000517:prevention & control*
chemical_list: D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; C587274:Qing Ye Dan