title: Dextran-functionalized magnetic fluid mediating magnetohyperthermia for treatment of Ehrlich-solid-tumor-bearing mice: toxicological and histopathological evaluations.
abstract: Dextran-functionalized maghemite fluid (DexMF) has been tested to treat Ehrlich-solid-tumor-bearing mice, evidencing its potential use in mediating magnetohyperthermia in breast cancer treatment. However, although magnetic nanoparticles tend to accumulate in tumor tissues, part of the nanomaterial can reach the blood stream, and then the organism. The aim of this study was to investigate the acute systemic effects of the intratumoral injection of DexMF mediating magnetohyperthermia in the treatment of an advanced clinical Ehrlich-solid-tumor, assessed through histopathological analyses of liver, kidneys, heart and spleen, comet assay, micronucleus test, hemogram, and serum levels of bilirubin, aspartate aminotransferase, alanine aminotransferase, gamma glutamyl transferase, alkaline phosphatase, creatinine, and urea. The tumor's histopathology and morphometry were used to assess its aggressiveness and regression. DexMF mediating hyperthermia was effective in containing tumor aggressiveness and in inducing tumor regression, besides showing no toxic effects. Its physical characteristics also suggest that it is safe to use in other biomedical applications.
authors.affiliation: Institute of Biological Sciences, Department of Genetics and Morphology, University of Brasilia, Brasília, Brazil, mirandavilela@unb.br.
authors.forename: Ana Luisa
authors.initials: AL
authors.lastname: Miranda-Vilela
authors.forename: Kelly Reis
authors.initials: KR
authors.lastname: Yamamoto
authors.forename: Kely Lopes Caiado
authors.initials: KL
authors.lastname: Miranda
authors.forename: Breno Noronha
authors.initials: BN
authors.lastname: Matos
authors.forename: Marcos Célio
authors.initials: MC
authors.lastname: de Almeida
authors.forename: João Paulo Figueiró
authors.initials: JP
authors.lastname: Longo
authors.forename: José
authors.initials: J
authors.lastname: de Souza Filho
authors.forename: Juliana Menezes Soares
authors.initials: JM
authors.lastname: Fernandes
authors.forename: Patrícia Pommé Confessori
authors.initials: PP
authors.lastname: Sartoratto
authors.forename: Zulmira Guerrero Marques
authors.initials: ZG
authors.lastname: Lacava
mesh_terms: D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D020552:Comet Assay; D003911:Dextrans / Q000008:administration & dosage*; D005260:Female; D006979:Hyperthermia, Induced / Q000009:adverse effects / Q000379:methods*; D055909:Magnetic Field Therapy / Q000009:adverse effects / Q000379:methods*; D008279:Magnetic Resonance Imaging; D051379:Mice; D048629:Micronuclei, Chromosome-Defective; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: D003911:Dextrans; D005723:gamma-Glutamyltransferase