title: Microfluidic biosensor array with integrated poly(2,7-carbazole)/fullerene-based photodiodes for rapid multiplexed detection of pathogens.
abstract: A multiplexed microfluidic biosensor made of poly(methylmethacrylate) (PMMA) was integrated into an array of organic blend heterojunction photodiodes (OPDs) for chemiluminescent detection of pathogens. Waterborne Escherichia coli O157:H7, Campylobacter jejuni and adenovirus were targeted in the PMMA chip, and detection of captured pathogens was conducted by poly(2,7-carbazole)/fullerene OPDs which showed a responsivity over 0.20 A/W at 425 nm. The limits of chemiluminescent detection were 5 × 10(5) cells/mL for E. coli, 1 × 10(5) cells/mL for C. jejuni, and 1 × 10(-8) mg/mL for adenovirus. Parallel analysis for all three analytes in less than 35 min was demonstrated. Further recovery tests illustrated the potential of the integrated biosensor for detecting bacteria in real water samples.
authors.affiliation: Department of Micro and Nano Systems Technology, Faculty of Technology and Maritime Sciences, Vestfold University College, Box 2243, N-3103 Tonsberg, Norway. tao.dong@hive.no.
authors.forename: Nuno Miguel
authors.initials: NM
authors.lastname: Matos Pires
authors.forename: Tao
authors.initials: T
authors.lastname: Dong
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002227:Carbazoles / Q000737:chemistry*; D037741:Fullerenes / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D019904:Polymethyl Methacrylate / Q000737:chemistry; D014867:Water / Q000032:analysis; D014871:Water Microbiology
chemical_list: D002227:Carbazoles; D037741:Fullerenes; D014867:Water; C041514:carbazole; D019904:Polymethyl Methacrylate