title: Characterization and uncertainty evaluation of a new certified reference material of baicalein.
abstract: A new certified reference material (CRM) of baicalein was developed aiming to control the quality of baicalein and relative pharmaceuticals. Sample preparation, homogeneity, stability, value assignment, and uncertainty of a new certified reference material (CRM) of baicalein were presented in this paper. Characterization of the material relied on three different methods, which were differential scanning calorimetry (DSC), coulometric titration method (CT) and mass balance method. DSC and CT were used for purity determination of baicalein firstly. The certified value of baicalein CRM is 99.72% with an expanded uncertainty of 0.25% (k=2). The new CRM of baicalein can be used to validate analytical methods, improve the accuracy of measurement data as well as establish meteorological traceability of analytical results.
authors.affiliation: Beijing Key Laboratory of Polymorphic Drugs, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Ningbo
authors.initials: N
authors.lastname: Gong
authors.affiliation: Beijing Key Laboratory of Polymorphic Drugs, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Polymorphic Drugs, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Baoxi
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Shandong Key laboratory of Polymorphic Drugs, Tengzhou, Shandong 277500, China.
authors.forename: Zhaolin
authors.initials: Z
authors.lastname: Gao
authors.affiliation: Beijing Key Laboratory of Drug Targets and Screening Research, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. Electronic address: dugh@imm.ac.cn.
authors.forename: Guanhua
authors.initials: G
authors.lastname: Du
authors.affiliation: Beijing Key Laboratory of Polymorphic Drugs, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China; Shandong Key laboratory of Polymorphic Drugs, Tengzhou, Shandong 277500, China. Electronic address: luy@imm.ac.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lv
mesh_terms: D002152:Calorimetry, Differential Scanning / Q000379:methods; D044950:Flavanones / Q000737:chemistry*; D008970:Molecular Weight; D012015:Reference Standards; D035501:Uncertainty
chemical_list: D044950:Flavanones; C006680:baicalein
keywords: Baicalein; Certified reference material; Coulometric titration; Differential scanning calorimetry; Mass balance method