title: Staphylococcus aureus chronic intramammary infection modifies protein expression of transforming growth factor beta (TGF-β) subfamily components during active involution.
abstract: The objectives of this study were to determine whether Staphylococcus aureus chronic intramammary infection (IMI) influences protein expression of TGF-β subfamily components and collagen I and to examine the histomorphometric changes that occur in mammary stroma and parenchyma during active mammary gland involution. Twenty-one Holstein non-pregnant cows in late lactation either uninfected or with chronic natural S. aureus IMI were included in this study. Cows were slaughtered at 7, 14 and 21d after cessation of milking and samples for immunohistochemical and morphometric analysis were taken. Protein expression of TGF-β1, TGF-β2 and TGF-β3 was significantly higher in chronically infected quarters than in uninfected controls at the three involution stages studied. Immunostaining of TGF-βR1 and TGF-βR3 and collagen I was significantly higher in S. aureus-infected quarters than in uninfected controls at every involution time evaluated. The percentages of tissue area composed of parenchyma and intralobular stroma were significantly higher in S. aureus-infected than in uninfected quarters. Chronic S. aureus mastitis modifies protein expression of the three TGF-β isoforms and type 1 and 3 receptors, which was associated with changes directed to limit the scope of inflammation and injury to the host.
authors.affiliation: Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Argentina; Instituto de Ciencias Veterinarias del Litoral (ICIVET-Litoral), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: Carolina S
authors.initials: CS
authors.lastname: Andreotti
authors.affiliation: Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Argentina; Instituto de Ciencias Veterinarias del Litoral (ICIVET-Litoral), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: Elizabet A L
authors.initials: EA
authors.lastname: Pereyra
authors.affiliation: Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Argentina; Instituto de Ciencias Veterinarias del Litoral (ICIVET-Litoral), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: Celina
authors.initials: C
authors.lastname: Baravalle
authors.affiliation: Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Argentina; Instituto de Ciencias Veterinarias del Litoral (ICIVET-Litoral), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: María S
authors.initials: MS
authors.lastname: Renna
authors.affiliation: Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Argentina; Instituto de Ciencias Veterinarias del Litoral (ICIVET-Litoral), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: Hugo H
authors.initials: HH
authors.lastname: Ortega
authors.affiliation: Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria (INTA), C.C. 22 (2300) Rafaela, Santa Fe, Argentina; Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Argentina.
authors.forename: Luis F
authors.initials: LF
authors.lastname: Calvinho
authors.affiliation: Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Argentina; Instituto de Ciencias Veterinarias del Litoral (ICIVET-Litoral), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Electronic address: bdallard@fcv.unl.edu.ar.
authors.forename: Bibiana E
authors.initials: BE
authors.lastname: Dallard
mesh_terms: D000818:Animals; D002417:Cattle; D005260:Female; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry / Q000662:veterinary; D008321:Mammary Glands, Animal / Q000378:metabolism / Q000382:microbiology* / Q000648:ultrastructure; D008414:Mastitis, Bovine / Q000378:metabolism / Q000382:microbiology*; D020033:Protein Isoforms / Q000378:metabolism; D013203:Staphylococcal Infections / Q000378:metabolism / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D020033:Protein Isoforms; D016212:Transforming Growth Factor beta
keywords: Involution; Mastitis; Staphylococcus aureus; Transforming growth factor beta