title: The clinicopathological significance of lamin A/C, lamin B1 and lamin B receptor mRNA expression in human breast cancer.
abstract: Lamin A/C (LMNA), lamin B1 (LMNB1) and lamin B receptor (LBR) have key roles in nuclear structural integrity and chromosomal stability. In this study, we have studied the relationships between the mRNA expressions of A-type lamins, LMNB1 and LBR and the clinicopathological parameters in human breast cancer. Samples of breast cancer tissues (n = 115) and associated non-cancerous tissue (ANCT; n = 30) were assessed using reverse transcription and quantitative PCR. Transcript levels were correlated with clinicopathological data. Higher levels of A-type lamins and LMNB1 mRNA expression were seen in ANCT. Higher lamin A/C expression was associated with the early clinical stage (TNM1 vs. TNM3 - 13 vs. 0.21; p = 0.0515), with better clinical outcomes (disease-free survival vs. mortality - 11 vs. 1; p = 0.0326), and with better overall (p = 0.004) and disease-free survival (p = 0.062). The expression of LMNB1 declined with worsening clinical outcome (disease-free vs. mortalities - 0.0011 vs. 0.000; p = 0.0177). LBR mRNA expression was directly associated with tumor grade (grade 1 vs. grade 3 - 0.00 vs. 0.00; p = 0.0479) and Nottingham Prognostic Index (NPI1 vs. NPI3 - 0.00 vs. 0.00; p = 0.0551). To the best of our knowledge, this is the first study to suggest such a role for A-type lamins, lamin B1 and LBR in human breast cancer, identifying an important area for further research.
authors.affiliation: The London Breast Institute, Princess Grace Hospital, London, UK.
authors.forename: Umar
authors.initials: U
authors.lastname: Wazir
authors.forename: Mai Hassan
authors.initials: MH
authors.lastname: Ahmed
authors.forename: Joanna M
authors.initials: JM
authors.lastname: Bridger
authors.forename: Amanda
authors.initials: A
authors.lastname: Harvey
authors.forename: Wen G
authors.initials: WG
authors.lastname: Jiang
authors.forename: Anup K
authors.initials: AK
authors.lastname: Sharma
authors.forename: Kefah
authors.initials: K
authors.lastname: Mokbel
mesh_terms: D001940:Breast / Q000378:metabolism / Q000473:pathology*; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D002453:Cell Cycle; D043171:Chromosomal Instability; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D034904:Lamin Type A / Q000235:genetics*; D034921:Lamin Type B / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D000097682:Lamin B Receptor
chemical_list: D034904:Lamin Type A; D034921:Lamin Type B; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D000097682:Lamin B Receptor