title: Simultaneous analysis and peroxynitrite-scavenging activity of galloylated flavonoid glycosides and ellagic acid in Euphorbia supina.
abstract: The herbs of Euphorbia supina (Euphorbiaceae) have been used to treat hemorrhage, chronic bronchitis, hepatitis, jaundice, diarrhea, gastritis, and hemorrhoids as a medicinal herb. This work is aimed to qualitatively and quantitatively analyze the polyphenols with peroxynitrite-scavenging activities. The eight compounds: gallic acid, methyl gallate, avicularin, astragalin, juglanin, isoquercitrin 6″-gallate, astragalin 6″-gallate, and ellagic acid, were isolated from E. supina and used for HPLC analysis and peroxynitrite (ONOO(-))-scavenging assay. Simultaneous analysis of the eight compounds was performed on MeOH extract and its fractions. The contents in MeOH extract and peroxynitrite-scavenging activities of the dimer of gallic acid, ellagic acid (15.64 mg/g; IC50 0.89 μM), and two galloylated flavonoid glycosides, astragalin 6″-gallate (13.72 mg/g; IC50 1.43 μM) and isoquercitrin 6″-gallate (16.99 mg/g; IC50 1.75 μM), were high, compared to other compounds. The legendary uses of E. supina could be attributed to the high content of polyphenols, particularly ellagic acid, isoquercitrin 6″-gallate, and astragalin 6″-gallate as active principles.
authors.affiliation: Department of Applied Plant Sciences, Graduate School, Sangji University, Wonju, 220-702, Korea.
authors.forename: Agung
authors.initials: A
authors.lastname: Nugroho
authors.forename: Tae-Jin
authors.initials: TJ
authors.lastname: Rhim
authors.forename: Moo-Young
authors.initials: MY
authors.lastname: Choi
authors.forename: Jae Sue
authors.initials: JS
authors.lastname: Choi
authors.forename: Youn-Chul
authors.initials: YC
authors.lastname: Kim
authors.forename: Min-Sun
authors.initials: MS
authors.lastname: Kim
authors.forename: Hee-Juhn
authors.initials: HJ
authors.lastname: Park
mesh_terms: D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D004610:Ellagic Acid / Q000032:analysis* / Q000737:chemistry; D028481:Euphorbia; D005419:Flavonoids / Q000032:analysis* / Q000378:metabolism; D016166:Free Radical Scavengers / Q000032:analysis / Q000378:metabolism; D006027:Glycosides / Q000032:analysis* / Q000737:chemistry; D030421:Peroxynitrous Acid / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D016166:Free Radical Scavengers; D006027:Glycosides; D030421:Peroxynitrous Acid; D004610:Ellagic Acid