title: In vitro effects of a chemically modified titanium surface on ethanol-exposed osteoblasts.
abstract: OBJECTIVE
  No clinical evidence-based data on a possible negative influence of osteopenia and harmful lifestyles on implant osseointegration have been published to date. However, chronic alcohol abuse has been related to a progressive osteoporotic state. The continuous effort on developing better performing surfaces might offer improvement in osseointegration rates in conditions of poor bone quality. The aim of the present study was to investigate, in vitro, the behavior of osteoblasts obtained from rats exposed to ethanol vapor for 7 weeks and cultured on an innovative titanium surface achieved by a double anodic spark deposition.
  
  
  METHODS
  Ethanol-exposed and unexposed (control) osteoblasts were cultured on an anodic spark-deposited titanium surface and compared to those cultured on an acid-etched one. Cell proliferation and synthesis of alkaline phosphatase, osteocalcin, collagen I, transforming growth factor-β1, interleukin-6, and tumor necrosis factor-α were measured at 3, 7, and 14 days of culture.
  
  
  RESULTS
  When cultured on the anodic spark-deposited titanium surface, ethanol-exposed osteoblasts recovered proliferation impairment. Anodic spark-deposited titanium surfaces significantly improved both ethanol-exposed and unexposed osteoblast bioactivity, particularly with regard to alkaline phosphatase and transforming growth factor-β1. Moreover, interleukin-6 release for ethanol-exposed osteoblasts was significantly reduced by the anodic spark-deposited titanium surface.
  
  
  CONCLUSIONS
  Proliferative and synthetic cell processes altered by ethanol contact were positively and partially restored by contact with the tested innovative surface.
authors.forename: Paola
authors.initials: P
authors.lastname: Torricelli
authors.forename: Milena
authors.initials: M
authors.lastname: Fini
authors.forename: Gianluca
authors.initials: G
authors.lastname: Giavaresi
authors.forename: Roberto
authors.initials: R
authors.lastname: Rimondini
authors.forename: Roberto
authors.initials: R
authors.lastname: Chiesa
authors.forename: Lia
authors.initials: L
authors.lastname: Rimondini
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology*; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D016348:Osseointegration / Q000187:drug effects* / Q000502:physiology; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D015675:Osteocalcin / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D015675:Osteocalcin; D000431:Ethanol; D014025:Titanium; D000469:Alkaline Phosphatase