title: Antifungal Effect of Brachyglottis repanda Ethanol Extract.
abstract: The crude ethanol extract of B. repanda showed the cytotoxic activity against Polio virus (25% activity at 150 μg/disk) and the minor cytotoxic activity against BSC cells (African green monkey kidney) . However, the crude ethanol extract of B. repanda was non-toxic to murine leukaemia cells CCL 46 P388D1 (IC50, > 62,500 ng/ml) . Cytotoxic and antifungal activities were strongly shown by Fr. 64-3 which was eluted with 90% CH3CN/H2O, 100% CH3CN, and 50% CH3CN/H2O (SM 2 at 150 μg/disk) . The fraction 64-3 also showed the most cytotoxic activity against murine leukaemia cells (128 mg, IC50 10,051 ng/ml at 75 μg/disk) . These results suggest that this fraction has a potent antifungal activity against the dermatophytic fungus Trichophyton mentagrophytes ATCC 28185.
authors.affiliation: Department of Food Science, Wonkwang Health Science, Iksan 570-750.
authors.forename: Chan Nam
authors.initials: CN
authors.lastname: Yook
authors.forename: Young Soon
authors.initials: YS
authors.lastname: Na
authors.forename: Hwa Jung
authors.initials: HJ
authors.lastname: Choi
authors.forename: Il Soo
authors.initials: IS
authors.lastname: You
authors.forename: Jong Min
authors.initials: JM
authors.lastname: Baek
authors.forename: Seung Hwa
authors.initials: SH
authors.lastname: Baek
keywords: Antifungal activity; Antiviral activity; Brachyglottis repanda; Cytotoxic activity; Murine leukaemia cell lines; Trichophyton mentagrophytes