title: In Vitro Evaluation of Antimicrobial Activity of Lactic Acid Bacteria against Clostridium difficile.
abstract: Clostridium difficile infection (CDI) has become a significant threat to public health. Although broad-spectrum antibiotic therapy is the primary treatment option for CDI, its use has evident limitations. Probiotics have been proved to be effective in the treatment of CDI and are a promising therapeutic option for CDI. In this study, 4 strains of lactic acid bacteria (LAB), namely, Lactobacillus rhamnosus (LR5), Lactococcuslactis (SL3), Bifidobacterium breve (BR3), and Bifidobacterium lactis (BL3) were evaluated for their anti-C. difficile activity. Co-culture incubation of C. difficile (10(6) and 10(10) CFU/ml) with each strain of LAB indicated that SL3 possessed the highest antimicrobial activity over a 24-hr period. The cell-free supernatants of the 4 LAB strains exhibited MIC50 values between 0.424 mg/ml (SL3) and 1.318 (BR3) mg/ml. These results may provide a basis for alternative therapies for the treatment of C. difficile-associated gut disorders.
authors.affiliation: R&D Center, CellBiotech, Co. Ltd., Gyunggi, Korea.
authors.forename: Joong-Su
authors.initials: JS
authors.lastname: Lee
authors.forename: Myung-Jun
authors.initials: MJ
authors.lastname: Chung
authors.forename: Jae-Gu
authors.initials: JG
authors.lastname: Seo
keywords: Clostridium difficile infection; antimicrobial activity; cell-free supernatant; lactic acid bacteria