title: Voltammetric behavior of uric acid on carbon paste electrode modified with salmon sperm dsDNA and its application as label-free electrochemical sensor.
abstract: A simple and sensitive label-free electrochemical DNA biosensor was proposed for the rapid determination of uric acid (UA) using a carbon nano tube paste electrode (CNTPE) modified with salmon sperm dsDNA. At first, the interaction between UA and the DNA was studied using differential pulse voltammetry (DPV). The addition of the DNA to UA solution resulted in a decrease in the peak current of UA and at the same time, a positive shift in the peak potential indicating an intercalative interaction. Then, the voltammetric response of a DNA-immobilized CNTPE was investigated for the determination of UA. The immobilization of the DNA was carried out using acid-functionalized carbon nanotubes and studied using Fe(CN)6(3-)/Fe(CN)6(4-) redox indicator. Compared with unmodified CNTPE, the oxidation signal of UA showed a significant increase at the DNA-coated electrode, and shifted to more positive potentials attributed to the pre-concentration of UA at the electrode surface due to interaction with the surface-confined DNA layer. This interaction was used for the fabrication of a simple and sensitive biosensor for determining UA. After the optimization of operational parameters, a linear dependence of the peak current on the UA concentration was observed in the range of 7.0×10(-7) to 1.1×10(-4) mol L(-1), with the detection and quantification limits of 1.8×10(-7) and 5.8×10(-7) mol L(-1), respectively. The proposed biosensor was successfully applied to validate its capability for the analysis of UA in human serum and urine samples.
authors.affiliation: Department of Chemistry, Shahid Bahonar University of Kerman, Kerman, Iran; Young Research Society, Shahid Bahonar University of Kerman, Kerman, Iran. Electronic address: m.mohamadi794@gmail.com.
authors.forename: Maryam
authors.initials: M
authors.lastname: Mohamadi
authors.affiliation: Department of Chemistry, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Mostafavi
authors.affiliation: Department of Biotechnology, Institute of Science, High Technology and Environmental Science, Graduate University of Advance Technology, Kerman, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Torkzadeh-Mahani
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D004247:DNA / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D006801:Humans; D057230:Limit of Detection; D008297:Male; D037742:Nanotubes, Carbon / Q000737:chemistry*; D012474:Salmon; D013094:Spermatozoa / Q000737:chemistry; D014527:Uric Acid / Q000032:analysis / Q000097:blood* / Q000652:urine*
chemical_list: D037742:Nanotubes, Carbon; D014527:Uric Acid; D004247:DNA
keywords: Carbon nano tube paste electrode; DNA biosensor; Salmon sperm dsDNA; Uric acid; Voltammetry