title: Whole blood transcriptome comparison of pigs with extreme production of in vivo dsRNA-induced serum IFN-a.
abstract: Interferon (IFN) is one of the major regulators of innate immunity, it also mediates the adaptive immune responses to a broad spectrum of pathogens. This study aims in identifying differences between high vs. low INF-a responders which were chosen based on serum INF-a levels at 4 h post poly I:C treatment. A transcriptomic analysis was designed to describe the whole blood differential transcriptomal response to poly I:C by pigs with high vs. low IFN alpha levels. The capability of producing dsRNA (poly I:C)-induced serum IFN-a is highly variable in pig population. The high INF-a responders had 328 unique differentially expressed genes, suggesting that the HIGH pigs have greater responsiveness upon the dsRNA simulation. Based on the results, the interferon-dependent antiviral responsiveness through the IFN-stimulated genes (ISGs) is likely more effective in HIGH pigs. Inferring from the known organization of IFN pathways, the reason for the more IFN-a production in the HIGH pigs was likely due to the enhanced expression of IRF-7 in TLR or RIG- I/MDA5 signaling pathways. Furthermore, the larger number of the altered genes in the HIGH pigs after simulation is also possibly because of the greater number of the altered transcription factors. To our knowledge, this is the first report of comparative transcriptomic analysis to advance our understanding of whole blood immune response in pigs with different in vivo poly I:C-inducted IFN-a levels. The paper significantly expands our knowledge of how pigs respond to poly I:C which is highly relevant for understanding resistance to viral infections and also for vaccine development.
authors.affiliation: Key Lab of Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, Hubei, China; State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Lab of Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, Hubei, China; Institute of Animal Husbandry and Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.affiliation: Key Lab of Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
authors.forename: Songbai
authors.initials: S
authors.lastname: Yang
authors.affiliation: Key Lab of Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Lab of Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
authors.forename: Anjing
authors.initials: A
authors.lastname: Xiang
authors.affiliation: Key Lab of Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Cao
authors.affiliation: Key Lab of Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Fan
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, China.
authors.forename: Zhenfang
authors.initials: Z
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
authors.forename: Junlong
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Key Lab of Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Key Lab of Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, Hubei, China. Electronic address: zhumengjin@mail.hzau.edu.cn.
authors.forename: Mengjin
authors.initials: M
authors.lastname: Zhu
mesh_terms: D000818:Animals; D007113:Immunity, Innate; D050839:Interferon Regulatory Factor-7 / Q000378:metabolism*; D016898:Interferon-alpha / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D046228:Microarray Analysis; D011070:Poly I-C / Q000276:immunology; D012330:RNA, Double-Stranded / Q000378:metabolism; D015398:Signal Transduction; D013552:Swine / Q000276:immunology*; D059467:Transcriptome / Q000276:immunology; D014777:Virus Diseases / Q000276:immunology* / Q000628:therapy
chemical_list: D050839:Interferon Regulatory Factor-7; D016898:Interferon-alpha; D012330:RNA, Double-Stranded; D011070:Poly I-C
keywords: Blood; IFN-a; Pig; Poly I:C; Transcriptome; dsRNA