title: Napsin A is possibly useful marker to predict the tumorigenic potential of lung bronchiolo-alveolar hyperplasia in F344 rats.
abstract: There are 2 types of bronchiolo-alveolar hyperplasia found in rat lungs. One is 'inflammatory hyperplasia' with a potential to recover in future with removal of the stimulating insult and the other is 'latent tumorigenic hyperplasia' as an independent preneoplastic lesion for adenocarcinoma. In the present experiment, we focused on rat lung bronchiolo-alveolar hyperplasia induced by 4-(methyl-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK), which decreases with time after induction and reverts to normal, or by N-bis(2-hydroxypropyl)nitrosamine (DHPN), with tumorigenic potential to progress to adenoma and adenocarcinoma. Though NNK is a typical carcinogen inducing lung adenocarcinoma in female A/J mice, the tumorigenic potential by NNK in rats is weak. Differences between hyperplasias induced by DHPN and by NNK were here examined immunohistochemically. Formalin fixed paraffin embedded lung samples with hyperplastic and inflammatory lesions were obtained from rats exposed to DHPN or NNK and from lung inflammation models induced with fine particles like CuO, NiO and quartz. The 19 markers were examined immunohistochemically. Napsin A, in the inflammatory lesions and hyperplasia induced by NNK, was positive for macrophages and secretions in the alveoli spaces but less so in the walls of the alveoli. In the proliferative lesions including hyperplasia induced by DHPN, strong positive staining for napsin A was observed in the walls of the alveoli. Thus high expression was suggested to be possibly useful for detecting tumorigenic potential of rat lung hyperplasia.
authors.affiliation: Onco-Pathology, Department of Pathology and Host-Defense, Kagawa University, Kagawa 761-0793, Japan.
authors.forename: Masanao
authors.initials: M
authors.lastname: Yokohira
authors.affiliation: Onco-Pathology, Department of Pathology and Host-Defense, Kagawa University, Kagawa 761-0793, Japan.
authors.forename: Sosuke
authors.initials: S
authors.lastname: Kishi
authors.affiliation: Onco-Pathology, Department of Pathology and Host-Defense, Kagawa University, Kagawa 761-0793, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Yamakawa
authors.affiliation: Onco-Pathology, Department of Pathology and Host-Defense, Kagawa University, Kagawa 761-0793, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Nakano
authors.affiliation: Onco-Pathology, Department of Pathology and Host-Defense, Kagawa University, Kagawa 761-0793, Japan.
authors.forename: Fumiko
authors.initials: F
authors.lastname: Ninomiya
authors.affiliation: Shikoku Cytopathology Laboratory, Kagawa 761-8071, Japan.
authors.forename: Shigemi
authors.initials: S
authors.lastname: Kinouch
authors.affiliation: Shikoku Cytopathology Laboratory, Kagawa 761-8071, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Tanizawa
authors.affiliation: Onco-Pathology, Department of Pathology and Host-Defense, Kagawa University, Kagawa 761-0793, Japan.
authors.forename: Kousuke
authors.initials: K
authors.lastname: Saoo
authors.affiliation: Onco-Pathology, Department of Pathology and Host-Defense, Kagawa University, Kagawa 761-0793, Japan. Electronic address: imaida@med.kagawa-u.ac.jp.
authors.forename: Katsumi
authors.initials: K
authors.lastname: Imaida
mesh_terms: D000230:Adenocarcinoma / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D000077192:Adenocarcinoma of Lung; D000236:Adenoma / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D055745:Bronchioles / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006965:Hyperplasia; D007150:Immunohistochemistry; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D008175:Lung Neoplasms / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D009602:Nitrosamines / Q000494:pharmacology; D011379:Prognosis; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D014408:Biomarkers, Tumor; D009602:Nitrosamines; C012457:diisopropanolnitrosamine; C016583:4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone; D016282:Aspartic Acid Endopeptidases; C402635:Napsa protein, rat
keywords: Carcinogenesis; DHPN; Lung; NNK; Napsin A; Rat