title: Monte Carlo simulation of in vivo Raman spectral measurements of human skin with a multi-layered tissue optical model.
abstract: Raman photon generation inside human skin and escaping to skin surface were modeled in an eight-layered skin optical model. Intrinsic Raman spectra of different skin layers were determined by microscopy measurements of excised skin tissue sections. Monte Carlo simulation was used to study the excitation light distribution and intrinsic Raman signal distortion caused by tissue reabsorption and scattering during in vivo measurements. The simulation results demonstrated how different skin layers contributed to the observed in vivo Raman spectrum. Using the strongest Raman peak at 1445 cm(-1) as an example, the simulation suggested that the integrated contributions of the stratum corneum layer is 1.3%, the epidermis layer 28%, the dermis layer 70%, and the subcutaneous fat layer 1.1%. Reasonably good matching between the calculated spectrum and the measured in vivo Raman spectra was achieved, thus demonstrated great utility of our modeling method and approaches for help understanding the clinical measurements.
authors.affiliation: Institute of Photonics and Photon-Technology, The cultivation base of the state key laboratory of optoelectronic technology and functional materials co-constructed by Ministry of Education and Shaanxi Province, Northwest University, Xi'an, Shaanxi, China; Imaging Unit - Integrative Oncology Department, British Columbia Cancer Agency Research Centre, Vancouver, BC, Canada.
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhao
authors.forename: Harvey
authors.initials: H
authors.lastname: Lui
authors.forename: Qingli
authors.initials: Q
authors.lastname: He
authors.forename: Jintao
authors.initials: J
authors.lastname: Bai
authors.forename: Haishan
authors.initials: H
authors.lastname: Zeng
mesh_terms: D006801:Humans; D007259:Infrared Rays; D008954:Models, Biological; D009010:Monte Carlo Method; D055620:Optical Phenomena; D012867:Skin / Q000166:cytology*; D013059:Spectrum Analysis, Raman
keywords: Intrinsic Raman of skin; Monte Carlo simulation; Skin optical model; Tissue Raman spectroscopy