title: miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1.
abstract: Dendritic cells (DCs) are critical to initiate the immune response and maintain tolerance, depending on different status and subsets. The expression profiles of microRNAs (miRNAs) in various DC subsets and haematopoietic stem cells (HSCs), which generate DCs, remain to be fully identified. Here we examine miRNomes of mouse bone marrow HSCs, immature DCs, mature DCs and IL-10/NO-producing regulatory DCs by deep sequencing. We identify numerous stage-specific miRNAs and histone modification in HSCs and DCs at different differentiation stages. miR-30b, significantly upregulated via a TGF-beta/Smad3-mediated epigenetic pathway in regulatory DCs, can target Notch1 to promote IL-10 and NO production, suggesting that miR-30b is a negative regulator of immune response. We also identify miRNomes of in vivo counterparts of mature DCs and regulatory DCs and systematically compare them with DCs cultured in vitro. These results provide a resource for studying roles of miRNAs in stem cell biology, development and functional regulation of DC subsets.
authors.affiliation: 1] National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University, Shanghai 200433, China [2].
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Su
authors.forename: Cheng
authors.initials: C
authors.lastname: Qian
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jin
authors.initials: J
authors.lastname: Hou
authors.forename: Yan
authors.initials: Y
authors.lastname: Gu
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Han
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Chen
authors.forename: Minghong
authors.initials: M
authors.lastname: Jiang
authors.forename: Xuetao
authors.initials: X
authors.lastname: Cao
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000502:physiology*; D044127:Epigenesis, Genetic; D005786:Gene Expression Regulation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology*; D006657:Histones / Q000378:metabolism; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D051881:Receptor, Notch1 / Q000235:genetics / Q000378:metabolism*; D051900:Smad3 Protein / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D006657:Histones; C556062:IL10 protein, mouse; D035683:MicroRNAs; C534715:Mirn30d microRNA, mouse; C497762:Notch1 protein, mouse; D051881:Receptor, Notch1; D051900:Smad3 Protein; C497791:Smad3 protein, mouse; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D009569:Nitric Oxide