title: A comparative study of in vitro cytotoxic, antioxidant, and antimicrobial activity of Pt(II), Zn(II), Cu(II), and Co(III) complexes with N-heteroaromatic Schiff base (E)-2-[N'-(1-pyridin-2-yl-ethylidene)hydrazino]acetate.
abstract: In search for novel biologically active metal based compounds, an evaluation of in vitro cytotoxic, antioxidant, and antimicrobial activity of new Pt(II) complex and its Zn(II), Cu(II), and Co(III) analogues, with NNO tridentately coordinated N-heteroaromatic Schiff base ligand (E)-2-[N'-(1-pyridin-2-yl-ethylidene)hydrazino]acetate, was performed. Investigation of antioxidative properties showed that all of the compounds have strong radical scavenging potencies. The Zn(II) complex showed potent inhibition of DNA cleavage by hydroxyl radical. A cytotoxic action of investigated compounds was evaluated on cultures of human promyelocitic leukaemia (HL-60), human glioma (U251), rat glioma (C6), and mouse melanoma (B16) cell lines. It was shown that binuclear pentacoordinated Zn(II) complex possesses a strong dose-dependent cytotoxic activity, of the same order of magnitude as cisplatin on B16, C6, and U251 cells. Furthermore, Zn(II) complex causes oxidative stress-induced apoptotic death of HL-60 leukemic cells, associated with caspase activation, phosphatidylserine externalization, and DNA fragmentation.
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agriculture, University of Belgrade, Belgrade, Serbia.
authors.forename: Nenad R
authors.initials: NR
authors.lastname: Filipović
authors.forename: Ivanka
authors.initials: I
authors.lastname: Marković
authors.forename: Dragana
authors.initials: D
authors.lastname: Mitić
authors.forename: Natalija
authors.initials: N
authors.lastname: Polović
authors.forename: Miloš
authors.initials: M
authors.lastname: Milčić
authors.forename: Marija
authors.initials: M
authors.lastname: Dulović
authors.forename: Maja
authors.initials: M
authors.lastname: Jovanović
authors.forename: Milena
authors.initials: M
authors.lastname: Savić
authors.forename: Miomir
authors.initials: M
authors.lastname: Nikšić
authors.forename: Katarina
authors.initials: K
authors.lastname: Anđelković
authors.forename: Tamara
authors.initials: T
authors.lastname: Todorović
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D003035:Cobalt / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006801:Humans; D051379:Mice; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry* / Q000494:pharmacology*; D009944:Organoplatinum Compounds / Q000737:chemistry / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D012545:Schiff Bases; D015032:Zinc / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D000975:Antioxidants; D009942:Organometallic Compounds; D009944:Organoplatinum Compounds; D012545:Schiff Bases; D003035:Cobalt; D003300:Copper; D015032:Zinc
keywords: Apoptosis; Cytotoxic Activity; N-heteroaromatic Schiff Base; Oxidative Stress; Zinc