title: Stimulation of embryogenesis and haploid production in Brassica campestris anther cultures by elevated temperature treatments.
abstract: Culture of Brassica campestris anthers at 35°C for one or three days prior to culture at 25°C significantly stimulated the yield of microspore-derived embryos. More than 100 plants were regenerated from cultured embryos and haploids were identified amongst them. The haploid frequency was greater than 70% if all small-flowered sterile plants were considered to be haploid. The yield of microspore-derived plants in B. campestris is approaching the level where anther culture may be utilized as a practical breeding tool.
authors.affiliation: Ottawa Research Station, Research Branch, Agriculture Canada, Ottawa, Ontario, Canada.
authors.forename: W A
authors.initials: WA
authors.lastname: Keller
authors.forename: K C
authors.initials: KC
authors.lastname: Armstrong