title: Simultaneous determination of five bioactive secolignans in rat plasma by LC-MS/MS for pharmacokinetic studies following oral administration of Peperomia dindygulensis Miq. extract.
abstract: A rapid and sensitive ultra fast performance liquid chromatography-tandem mass spectrometry method was developed for the simultaneous determination of five bioactive secolignans in Peperomia dindygulensis extract, including peperomin A, peperomin B, peperomin C, 4″-hydroxypeperomin B and 4″-hydroxypeperomin C in rat plasma. Arctigenin was used as the internal standard. The separation was performed on an Innovation™ Polar-RP C18 column by a gradient elution within a runtime of 7min. The mobile phase consisted of A (methanol) and B (0.1% formic acid in water) at a flow rate of 0.4mL/min. The detection was accomplished by using positive ion TurboIonSpray ionization in multiple reaction monitoring mode. The method was linear for all analytes over investigated range with all correlation coefficients greater than 0.9972. The lower limits of quantification were 1.1ng/mL for peperomin A, 1.24ng/mL for peperomin B, 1.02ng/mL for peperomin C, 1.91ng/mL for 4″-hydroxypeperomin B and 1.27ng/mL for 4″-hydroxypeperomin C. The intra- and inter-day precision (RSD%) was within 15% and the accuracy (RE%) ranged from -11.7% to 10.3%. This simple and sensitive method was fully validated and successfully applied to the pharmacokinetic study of peperomin A, peperomin B, peperomin C, 4″-hydroxypeperomin B and 4″-hydroxypeperomin C in rat plasma after oral administration of P. dindygulensis extract.
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicines, Xianlin Avenue No. 138, 210023 Nanjing, China.
authors.forename: Xin-zhi
authors.initials: XZ
authors.lastname: Wang
authors.affiliation: Department of Natural Medicinal Chemistry, China Pharmaceutical University, Tongjia Lane No. 24, 210009 Nanjing, China.
authors.forename: Jing-yu
authors.initials: JY
authors.lastname: Liang
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicines, Xianlin Avenue No. 138, 210023 Nanjing, China. Electronic address: njwenhm@126.com.
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Wen
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicines, Xianlin Avenue No. 138, 210023 Nanjing, China.
authors.forename: Chen-xiao
authors.initials: CX
authors.lastname: Shan
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicines, Xianlin Avenue No. 138, 210023 Nanjing, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D017705:Lignans / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D008297:Male; D036941:Peperomia / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D017705:Lignans; D010936:Plant Extracts
keywords: Peperomia dindygulensis; Pharmacokinetics; Rat plasma; Secolignans; UFLC–MS/MS