title: Therapeutic effect of a TM4SF5-specific peptide vaccine against colon cancer in a mouse model.
abstract: Molecular-targeted therapy has gained attention because of its high efficacy and weak side effects. Previously, we confirmed that transmembrane 4 superfamily member 5 protein (TM4SF5) can serve as a molecular target to prevent or treat hepatocellular carcinoma (HCC). We recently extended the application of the peptide vaccine, composed of CpG-DNA, liposome complex, and TM4SF5 peptide, to prevent colon cancer in a mouse model. Here, we first implanted mice with mouse colon cancer cells and then checked therapeutic effects of the vaccine against tumor growth. Immunization with the peptide vaccine resulted in robust production of TM4SF5-specific antibodies, alleviated tumor growth, and reduced survival rate of the tumor-bearing mice. We also found that serum levels of VEGF were markedly reduced in the mice immunized with the peptide vaccine. Therefore, we suggest that the TM4SF5-specific peptide vaccine has a therapeutic effect against colon cancer in a mouse model.
authors.affiliation: Center for Medical Science Research, College of Medicine, Hallym University, Chuncheon 200-702, Korea.
authors.forename: Sanghoon
authors.initials: S
authors.lastname: Kwon
authors.affiliation: Department of Biochemistry, College of Natural Sciences, Chungbuk National University, Cheongju 361-763, Korea.
authors.forename: Young-Eun
authors.initials: YE
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, College of Natural Sciences, Chungbuk National University, Cheongju 361-763, Korea.
authors.forename: Jeong-A
authors.initials: JA
authors.lastname: Park
authors.affiliation: Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors.forename: Doo-Sik
authors.initials: DS
authors.lastname: Kim
authors.affiliation: Center for Medical Science Research; Department of Microbiology, College of Medicine, Hallym University, Chuncheon 200-702, Korea.
authors.forename: Hyung-Joo
authors.initials: HJ
authors.lastname: Kwon
authors.affiliation: Department of Biochemistry, College of Natural Sciences, Chungbuk National University, Cheongju 361-763, Korea.
authors.forename: Younghee
authors.initials: Y
authors.lastname: Lee
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000737:chemistry / Q000276:immunology* / Q000627:therapeutic use*; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D008565:Membrane Proteins / Q000037:antagonists & inhibitors* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D058990:Molecular Targeted Therapy; D009374:Neoplasms, Experimental / Q000188:drug therapy / Q000276:immunology / Q000473:pathology; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D019496:Cancer Vaccines; D008565:Membrane Proteins; C570858:TM4SF5 protein, mouse