title: Early induction of stress-associated Src activator/Homo sapiens chromosome 9 open reading frame 10 protein following photodynamic therapy.
abstract: BACKGROUND
  There are proteins, responsible for many basic cell functions (transmission of extracellular signals to cytoplasm or nucleus, cell growth, proliferation, migration, survival), which are activated and overexpressed in response to acute oxidative stress, especially tyrosine kinases. The oxidative stress-associated Src activator/Homo sapiens chromosome 9 open reading frame 10 protein (Ossa/C9orf10) protects cancer cells from oxidative stress-induced apoptosis by Src family kinases activation.
  
  
  METHODS
  In this study precursor of protoporphyrin IX, 5-aminolevulinic acid and its encapsulated form were used in treating MCF-7 human breast cancer cells. After light illumination, cells were collected at different time points and used for evaluation (immunocytochemistry, Western blot analysis) of expression of above proteins, c-Src and Ossa.
  
  
  RESULTS
  Our results showed that 5-aminolevulinic acid-mediated photodynamic therapy caused decrease of c-Src expression at 7h after irradiation. The strongest expression was observed at 24h after treatment. Encapsulated form of 5-aminolevulinic acid in terms of PDT caused similar changes of expression of c-Src protein. Furthermore, we observed strong Ossa expression at 7h after treatment in comparison to very low expression at time points 0, 18 and 24h.
  
  
  CONCLUSIONS
  We would like to emphasize that our results showed high expression of Ossa at early time interval after PDT, which was accompanied by a low expression of c-Src kinase, what could protect cancer cells from PDT through activation of c-Src in response to oxidative stress.
authors.affiliation: Department of Pathology, Wrocław Medical University, 50-367 Wrocław, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Woźniak
authors.affiliation: Department of Medical Biochemistry, Wrocław Medical University, 50-367 Wrocław, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Hotowy
authors.affiliation: Department of Medical Biochemistry, Wrocław Medical University, 50-367 Wrocław, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Czapińska
authors.affiliation: Department of Pathology, Wrocław Medical University, 50-367 Wrocław, Poland.
authors.forename: Kamila
authors.initials: K
authors.lastname: Duś-Szachniewicz
authors.affiliation: Department of Medical Biochemistry, Wrocław Medical University, 50-367 Wrocław, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Szczuka
authors.affiliation: Department of Pathology, Wrocław Medical University, 50-367 Wrocław, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Gamian
authors.affiliation: Department of Medical Biochemistry, Wrocław Medical University, 50-367 Wrocław, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gamian
authors.affiliation: Department of Medical Biochemistry, Wrocław Medical University, 50-367 Wrocław, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Terlecki
authors.affiliation: Department of Pathology, Wrocław Medical University, 50-367 Wrocław, Poland. Electronic address: ziolkows@interia.pl.
authors.forename: Piotr
authors.initials: P
authors.lastname: Ziółkowski
mesh_terms: D000622:Aminolevulinic Acid / Q000494:pharmacology*; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002899:Chromosomes, Human, Pair 9 / Q000187:drug effects*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D061986:MCF-7 Cells; D016366:Open Reading Frames; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000494:pharmacology*; D016601:RNA-Binding Proteins / Q000187:drug effects*; D019061:src-Family Kinases / Q000187:drug effects
chemical_list: C534604:FAM120A protein, human; D017319:Photosensitizing Agents; D016601:RNA-Binding Proteins; D000622:Aminolevulinic Acid; D019061:src-Family Kinases
keywords: MCF-7 cells; Ossa; Oxidative stress; Photodynamic therapy; c-Src kinase