title: Hydroxyurea-induced mitotic synchronization in Allium sativum root meristems.
abstract: The synchronized divisions following a treatment with hydroxyurea (HU) - an inhibitor of DNA synthesis - were studied in root meristems of Allium sativum using two methods: autoradiography of median sections and morphological labeling with a cytokinesis inhibitor. It is shown that the second wave of mitoses is heterogeneous: it is composed mostly of cells which have been synchronized in the S phase by the HU treatment, of cells coming from the quiescent center stimulated to enter DNA synthesis and of cells which were not blocked by the 23 h HU treatment (slow cycling cells). It is also shown that the cell cycle following the first synchronized division is considerably shortened by the synchronization procedure.
authors.affiliation: Laboratoire de Biologie cellulaire, Faculté des Sciences pharmaceutiques et biologiques, 4, Avenue de l'Observatoire, F-75270, Paris Cedex 06, France.
authors.forename: E
authors.initials: E
authors.lastname: Clain
authors.forename: A
authors.initials: A
authors.lastname: Brulfert