title: Identification and characterization of novel and differentially expressed microRNAs in peripheral blood from healthy and mastitis Holstein cattle by deep sequencing.
abstract: MicroRNA (miRNA) mediates post-transcriptional gene regulation and plays an important role in regulating the development of immune cells and in modulating innate and adaptive immune responses in mammals, including cattle. In the present study, we identified novel and differentially expressed miRNAs in peripheral blood from healthy and mastitis Holstein cattle by Solexa sequencing and bioinformatics. In total, 608 precursor hairpins (pre-miRNAs) encoding for 753 mature miRNAs were detected. Statistically, 173 unique miRNAs (of 753, 22.98%) were identified that had significant differential expression between healthy and mastitis Holstein cattle (P < 0.001). Most differentially expressed miRNAs (118 of 173, 68.21%) belonged to the chemokine signaling pathway involved in the immune responses. This study expands the number of miRNAs known to be expressed in cattle. The patterns of miRNAs expression differed significantly between the peripheral blood from healthy and mastitis Holstein cattle, which provide important information on mastitis in miRNAs expression. Diverse miRNAs may play an important role in the treatment of mastitis in Holstein cattle.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University; Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi, 712100, China.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Li
authors.forename: Hongliang
authors.initials: H
authors.lastname: Wang
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Lijun
authors.initials: L
authors.lastname: Wang
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Liu
authors.forename: Caixia
authors.initials: C
authors.lastname: Ru
authors.forename: Ailong
authors.initials: A
authors.lastname: Song
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D018925:Chemokines / Q000276:immunology; D002874:Chromosome Mapping; D019295:Computational Biology; D005260:Female; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing; D008414:Mastitis, Bovine / Q000235:genetics* / Q000276:immunology; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D017423:Sequence Analysis, RNA; D015398:Signal Transduction / Q000235:genetics
chemical_list: D018925:Chemokines; D035683:MicroRNAs
keywords: bovine; immune-related; microRNA expression; solexa sequencing