title: Oxytocin differently regulates pressor responses to stress in WKY and SHR rats: the role of central oxytocin and V1a receptors.
abstract: The role of central oxytocin in the regulation of cardiovascular parameters under resting conditions and during acute stress was investigated in male normotensive Wistar-Kyoto (WKY; n = 40) and spontaneously hypertensive rats (SHR; n = 28). In Experiment 1, mean arterial blood pressure (MABP) and heart rate (HR) were recorded in WKY and SHR rats at rest and after an air-jet stressor during intracerebroventricular (ICV) infusions of vehicle, oxytocin or oxytocin receptor (OTR) antagonist. In Experiment 2, the effects of vehicle, oxytocin and OTR antagonist were determined in WKY rats after prior administration of a V1a vasopressin receptor (V1aR) antagonist. Resting MABP and HR were not affected by any of the ICV infusions either in WKY or in SHR rats. In control experiments (vehicle), the pressor response to stress was significantly higher in SHR. Oxytocin enhanced the pressor response to stress in the WKY rats but reduced it in SHR. During V1aR blockade, oxytocin infusion entirely abolished the pressor response to stress in WKY rats. Combined blockade of V1aR and OTR elicited a significantly greater MABP response to stress than infusion of V1a antagonist and vehicle. This study reveals significant differences in the regulation of blood pressure in WKY and SHR rats during alarming stress. Specifically, the augmentation of the pressor response to stress by exogenous oxytocin in WKY rats is caused by its interaction with V1aR, and endogenous oxytocin regulates the magnitude of the pressor response to stress in WKY rats by simultaneous interaction with OTR and V1aR.
authors.affiliation: Department of Experimental and Clinical Physiology, Medical University of Warsaw , Warsaw , Poland.
authors.forename: A
authors.initials: A
authors.lastname: Wsol
authors.forename: E
authors.initials: E
authors.lastname: Szczepanska-Sadowska
authors.forename: S
authors.initials: S
authors.lastname: Kowalewski
authors.forename: L
authors.initials: L
authors.lastname: Puchalska
authors.forename: A
authors.initials: A
authors.lastname: Cudnoch-Jedrzejewska
mesh_terms: D000818:Animals; D065092:Antidiuretic Hormone Receptor Antagonists; D001794:Blood Pressure / Q000187:drug effects*; D002319:Cardiovascular System / Q000187:drug effects; D006339:Heart Rate / Q000187:drug effects; D008297:Male; D010121:Oxytocin / Q000494:pharmacology*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D018045:Receptors, Oxytocin / Q000037:antagonists & inhibitors / Q000502:physiology*; D017483:Receptors, Vasopressin / Q000502:physiology*; D012146:Rest; D013315:Stress, Psychological
chemical_list: D065092:Antidiuretic Hormone Receptor Antagonists; D018045:Receptors, Oxytocin; D017483:Receptors, Vasopressin; C582098:V1a vasopressin receptor, rat; D010121:Oxytocin