title: Mechanism for the catastrophe-promoting activity of the microtubule destabilizer Op18/stathmin.
abstract: Regulation of microtubule dynamic instability is crucial for cellular processes, ranging from mitosis to membrane transport. Stathmin (also known as oncoprotein 18/Op18) is a prominent microtubule destabilizer that acts preferentially on microtubule minus ends. Stathmin has been studied intensively because of its association with multiple types of cancer, but its mechanism of action remains controversial. Two models have been proposed. One model is that stathmin promotes microtubule catastrophe indirectly, and does so by sequestering tubulin; the other holds that stathmin alters microtubule dynamics by directly destabilizing growing microtubules. Stathmin's sequestration activity is well established, but the mechanism of any direct action is mysterious because stathmin binds to microtubules very weakly. To address these issues, we have studied interactions between stathmin and varied tubulin polymers. We show that stathmin binds tightly to Dolastatin-10 tubulin rings, which mimic curved tubulin protofilaments, and that stathmin depolymerizes stabilized protofilament-rich polymers. These observations lead us to propose that stathmin promotes catastrophe by binding to and acting upon protofilaments exposed at the tips of growing microtubules. Moreover, we suggest that stathmin's minus-end preference results from interactions between stathmin's N terminus and the surface of α-tubulin that is exposed only at the minus end. Using computational modeling of microtubule dynamics, we show that these mechanisms could account for stathmin's observed activities in vitro, but that both the direct and sequestering activities are likely to be relevant in a cellular context. Taken together, our results suggest that stathmin can promote catastrophe by direct action on protofilament structure and interactions.
authors.affiliation: Department of Chemistry and Biochemistry, Department of Applied and Computational Mathematics and Statistics, Interdisciplinary Center for the Study of Biocomplexity, and Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556.
authors.forename: Kamlesh K
authors.initials: KK
authors.lastname: Gupta
authors.forename: Chunlei
authors.initials: C
authors.lastname: Li
authors.forename: Aranda
authors.initials: A
authors.lastname: Duan
authors.forename: Emily O
authors.initials: EO
authors.lastname: Alberico
authors.forename: Oleg V
authors.initials: OV
authors.lastname: Kim
authors.forename: Mark S
authors.initials: MS
authors.lastname: Alber
authors.forename: Holly V
authors.initials: HV
authors.lastname: Goodson
mesh_terms: D000818:Animals; D047630:Depsipeptides / Q000737:chemistry; D006801:Humans; D008870:Microtubules / Q000737:chemistry* / Q000378:metabolism; D056004:Molecular Dynamics Simulation; D011485:Protein Binding; D050777:Stathmin / Q000737:chemistry* / Q000378:metabolism; D013552:Swine; D014404:Tubulin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D047630:Depsipeptides; C493995:STMN1 protein, human; D050777:Stathmin; D014404:Tubulin; C064570:dolastatin 10
keywords: GMPCPP; T2S complex; Zn-sheets; computer simulation