title: Identification of disulfide bond formation between MitoNEET and glutamate dehydrogenase 1.
abstract: MitoNEET is a protein that was identified as a drug target for diabetes, but its cellular function as well as its role in diabetes remains elusive. Protein pull-down experiments identified glutamate dehydrogenase 1 (GDH1) as a potential binding partner. GDH1 is a key metabolic enzyme with emerging roles in insulin regulation. MitoNEET forms a covalent complex with GDH1 through disulfide bond formation and acts as an activator. Proteomic analysis identified the specific cysteine residues that participate in the disulfide bond. This is the first report that effectively links mitoNEET to activation of the insulin regulator GDH1.
authors.affiliation: Department of Chemistry, Eastern Illinois University , 600 West Lincoln Avenue, Charleston, Illinois 61920, United States.
authors.forename: Morgan E
authors.initials: ME
authors.lastname: Roberts
authors.forename: Jacquelyn P
authors.initials: JP
authors.lastname: Crail
authors.forename: Megan M
authors.initials: MM
authors.lastname: Laffoon
authors.forename: William G
authors.initials: WG
authors.lastname: Fernandez
authors.forename: Michael A
authors.initials: MA
authors.lastname: Menze
authors.forename: Mary E
authors.initials: ME
authors.lastname: Konkle
mesh_terms: D000818:Animals; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism*; D005969:Glutamate Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D033862:Iron-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D008099:Liver / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D024101:Mitochondrial Proteins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D017470:Receptors, Glutamate / Q000737:chemistry / Q000378:metabolism*
chemical_list: C525595:CISD1 protein, human; D004220:Disulfides; D033862:Iron-Binding Proteins; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D017470:Receptors, Glutamate; C519501:mitoNEET protein, mouse; C570817:GluD1 protein, mouse; D005969:Glutamate Dehydrogenase; C561840:GLUD1 protein, human