title: Induction of Th17 lymphocytes and Treg cells by monocyte-derived dendritic cells in patients with rheumatoid arthritis and systemic lupus erythematosus.
abstract: Dendritic cells (DCs) have a key role in the regulation of immune response. We herein explored, in patients with inflammatory diseases, the role of monocyte derived DC's (mo-DCs) on the generation of Th17 and T regulatory (Treg) lymphocytes. Peripheral blood was obtained from thirty-five patients with rheumatoid arthritis (RA), twelve with systemic lupus erythematosus (SLE), and twenty healthy subjects. Mo-DCs were generated under standard (IL-4/GM-CSF) or tolerogenic (IL-4/GM-CSF plus recombinant P-selectin or PD-1 or IL-10) conditions, and their ability to induce Th17 and Treg lymphocytes was tested. We detected that mo-DCs from patients with RA showed an enhanced release of IL-6 and IL-23 as well as an increased capability to induce Th17 cells. Although mo-DCs from SLE patients also released high levels of IL-6/IL-23, it did not show an increased ability to induce Th17 lymphocytes. In addition, mo-DCs, from patients with RA and SLE generated under the engagement of PSGL-1, showed a defective capability to induce Foxp3+ Treg cells. A similar phenomenon was observed in SLE, when DC's cells were generated under PDL-1 engagement. Our data indicate that DCs from patients with rheumatic inflammatory disease show an aberrant function that may have an important role in the pathogenesis of these conditions.
authors.affiliation: Department of Immunology, Facultad de Medicina, UASLP, Avenue V. Carranza 2405, 78210 San Luis Potosí, SLP, Mexico.
authors.forename: Lizbeth
authors.initials: L
authors.lastname: Estrada-Capetillo
authors.forename: Berenice
authors.initials: B
authors.lastname: Hernández-Castro
authors.forename: Adriana
authors.initials: A
authors.lastname: Monsiváis-Urenda
authors.forename: Crisol
authors.initials: C
authors.lastname: Alvarez-Quiroga
authors.forename: Esther
authors.initials: E
authors.lastname: Layseca-Espinosa
authors.forename: Carlos
authors.initials: C
authors.lastname: Abud-Mendoza
authors.forename: Lourdes
authors.initials: L
authors.lastname: Baranda
authors.forename: Ana
authors.initials: A
authors.lastname: Urzainqui
authors.forename: Francisco
authors.initials: F
authors.lastname: Sánchez-Madrid
authors.forename: Roberto
authors.initials: R
authors.lastname: González-Amaro
mesh_terms: D000293:Adolescent; D000328:Adult; D001172:Arthritis, Rheumatoid / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D008180:Lupus Erythematosus, Systemic / Q000276:immunology* / Q000378:metabolism; D008875:Middle Aged; D010641:Phenotype; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D058504:Th17 Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D055815:Young Adult
chemical_list: D016207:Cytokines