title: Enhancing lutein productivity of an indigenous microalga Scenedesmus obliquus FSP-3 using light-related strategies.
abstract: Lutein, one of the main photosynthetic pigments, is a promising natural product with both nutritional and pharmaceutical applications. In this study, light-related strategies were applied to enhance the cell growth and lutein production of a lutein-rich microalga Scenedesmus obliquus FSP-3. The results demonstrate that using white LED resulted in better lutein production efficiency when compared to the other three monochromatic LEDs (red, blue, and green). The lutein productivity of S. obliquus FSP-3 was further improved by adjusting the type of light source and light intensity. The optimal lutein productivity of 4.08 mg/L/d was obtained when using a TL5 fluorescent lamp at a light intensity of 300 μmol/m(2)/s, and this performance is better than that reported in most related studies. Moreover, the time-course profile of lutein accumulation in the microalga shows that the maximal lutein content and productivity were obtained at the onset of nitrogen depletion.
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, Kobe, Japan; Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Shih-Hsin
authors.initials: SH
authors.lastname: Ho
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Ming-Chang
authors.initials: MC
authors.lastname: Chan
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Chen-Chun
authors.initials: CC
authors.lastname: Liu
authors.affiliation: Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Chun-Yen
authors.initials: CY
authors.lastname: Chen
authors.affiliation: Department of Mathematics and Physics, Air Force Academy, Gangshan, Kaohsiung 820, Taiwan.
authors.forename: Wen-Lung
authors.initials: WL
authors.lastname: Lee
authors.affiliation: Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan; Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan.
authors.forename: Duu-Jong
authors.initials: DJ
authors.lastname: Lee
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan; Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan; Research Center for Energy Technology and Strategy Center, National Cheng Kung University, Tainan, Taiwan. Electronic address: changjs@mail.ncku.edu.tw.
authors.forename: Jo-Shu
authors.initials: JS
authors.lastname: Chang
mesh_terms: D061249:Batch Cell Culture Techniques; D018533:Biomass; D002245:Carbon Dioxide / Q000494:pharmacology; D008027:Light; D014975:Lutein / Q000096:biosynthesis*; D058086:Microalgae / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism* / Q000528:radiation effects*; D009584:Nitrogen / Q000378:metabolism / Q000494:pharmacology; D058433:Photobioreactors / Q000382:microbiology; D044444:Scenedesmus / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism* / Q000528:radiation effects*; D013997:Time Factors
chemical_list: D002245:Carbon Dioxide; D009584:Nitrogen; D014975:Lutein
keywords: Light intensity; Light source; Lutein; Microalgae; Scenedesmus obliquus