title: GammadeltaT cells positively regulate contact sensitivity (CS) reaction via modulation of INF-gamma, IL-12 and TNF-alpha production.
abstract: The gammadeltaT cells were identified as positive as well as negative regulators of immune responses. They take part in pathogen clearance, modulation of innate and adaptive immunity as well as in healing and tissue maintenance. The course of many pathological conditions such as collagen induced arthritis (CIA), experimental autoimmune encephalomyelitis (EAE) and airway hyperresponsiveness is positively regulated by gammadeltaT cells. It was shown previously that contact sensitivity (CS), an example of antigen-specific cell-mediated immune response, is also positively regulated by gammadeltaT cells. The current work confirmed the regulatory function of gammadeltaT cells in CS response as their depletion with anti-TCRdelta monoclonal antibody and complement significantly decreased adoptive transfer of the CS reaction. In vitro study showed that removal of gammadeltaT cells with magnetic beads significantly decreased the production of the proinflammatory cytokines IFN-gamma, IL-12 and TNF-alpha. Reconstitution of gammadeltaT-depleted cells with gammadeltaT-enriched cells restored cytokine production, proving the reversibility of the investigated process. In summary, gammadeltaT cells positively regulate the CS reaction via modulation of proinflammatory cytokine production.
authors.affiliation: Department of Medical Biology, Jagiellonian University College of Medicine, Kopernika 7, PL 31-034 Kraków, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Strzepa
authors.forename: Monika
authors.initials: M
authors.lastname: Majewska-Szczepanik
authors.forename: Marian
authors.initials: M
authors.lastname: Szczepanik
mesh_terms: D000818:Animals; D003877:Dermatitis, Contact / Q000276:immunology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism*; D018664:Interleukin-12 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics / Q000378:metabolism*; D013047:Specific Pathogen-Free Organisms; D013601:T-Lymphocytes / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D016692:Receptors, Antigen, T-Cell, gamma-delta; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D007371:Interferon-gamma