title: Antiviral Potential of Selected Starter Cultures, Bacteriocins and D,L-Lactic Acid.
abstract: The antiviral potential of selected bacteria species [lactic acid bacteria (LAB) and micrococcaceae] was examined. By this, the effect of their cell-free supernatants as well as of certain species-related metabolites (sakacin A, nisin, and lactic acid) was investigated on different viruses after exposure at 24 °C for 3 days. Viruses were incubated with supernatants and metabolites in a dilution ratio of 1:10. Data for antiviral effects towards murine norovirus S99 (MNV), influenza A virus A/WSN/33 (H1N1), Newcastle disease virus Montana (NDV) and feline herpesvirus KS 285 (FHV) were generated in vitro simulating pH and temperature conditions according to raw sausage fermentations. Investigations showed no antiviral effect of sakacin A and nisin on MNV, H1N1, FHV and NDV. Furthermore, the antiviral potential of D,L-lactic acid was determined for MNV and H1N1. At raw sausage-related pH values (5.0-6.2) it could be shown that the virus titre for MNV and H1N1 was reduced by a maximum of 3.25 log and 2.5 log units, respectively. In addition, 29 culture supernatants of different bacteria species, mainly LAB and staphylococci, were tested for their antiviral activity against MNV. Only the cell-free supernatant of a Lb. curvatus strain showed a higher virus titre reduction of MNV by 1.25 log units compared to the control. Further studies on the characterisation of this cell-free supernatant were carried out, however, the antiviral substance could not be identified so far.
authors.affiliation: Centre of Veterinary Public Health, Institute of Food Hygiene, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1, 04103, Leipzig, Germany. anett.lange@vetmed.uni-leipzig.de.
authors.forename: Anett
authors.initials: A
authors.lastname: Lange-Starke
authors.affiliation: Centre of Veterinary Public Health, Institute of Animal Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1, 04103, Leipzig, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Petereit
authors.affiliation: Centre of Veterinary Public Health, Institute of Animal Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1, 04103, Leipzig, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Truyen
authors.affiliation: Centre of Veterinary Public Health, Institute of Food Hygiene, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1, 04103, Leipzig, Germany.
authors.forename: P G
authors.initials: PG
authors.lastname: Braun
authors.affiliation: Centre of Veterinary Public Health, Institute of Food Hygiene, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1, 04103, Leipzig, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Fehlhaber
authors.affiliation: Centre of Veterinary Public Health, Institute of Food Hygiene, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1, 04103, Leipzig, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Albert
mesh_terms: D000998:Antiviral Agents / Q000378:metabolism / Q000494:pharmacology*; D001430:Bacteriocins / Q000378:metabolism / Q000494:pharmacology*; D003470:Culture Media / Q000378:metabolism / Q000494:pharmacology*; D019344:Lactic Acid / Q000378:metabolism / Q000494:pharmacology*; D007777:Lactobacillaceae / Q000737:chemistry* / Q000378:metabolism; D014780:Viruses / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000998:Antiviral Agents; D001430:Bacteriocins; D003470:Culture Media; D019344:Lactic Acid
keywords: Influenza virus; Murine norovirus; Raw sausage; Starter culture; Virus inactivation