title: Dietary trans-10,cis-12 CLA reduces murine collagen-induced arthritis in a dose-dependent manner.
abstract: Dietary trans-10,cis-12 (t10c12) conjugated linoleic acid (CLA) has been shown to reduce inflammation in a murine collagen-induced arthritis (CA) model. To understand the anti-inflammatory potential of t10c12-CLA in the diet, the minimum dose of pure dietary t10c12-CLA capable of reducing CA was investigated. Because plasma inflammatory cytokines often do not reflect the progression of late-stage arthritis, inflamed tissue cytokine concentrations were also investigated in relation to increasing dietary t10c12-CLA amounts. Mice were randomly assigned to the following dietary treatments upon the establishment of arthritis: corn oil (CO) or 0.125%, 0.25%, 0.375%, or 0.5% t10c12-CLA (wt:wt) for 84 d. Sham mice (no arthritis) were fed CO and served as controls. Arthritic paw score, based on subjective assessment of arthritic severity, and paw thickness decreased linearly overall [16-65% (P < 0.001) and 0.5-12% (P < 0.001), respectively] as dietary t10c12-CLA increased (P < 0.001, R(2) < 0.81). Increasing dietary t10c12-CLA was associated with a decrease in plasma interleukin (IL)-1β at days 21 and 42 compared with CO-fed arthritic mice, such that mice fed ≥0.25% t10c12-CLA had IL-1β concentrations that were similar to sham mice. Plasma cytokines returned to sham mice concentrations by day 63 regardless of treatment; however, an arthritis-induced elevation in paw IL-1β decreased linearly as dietary t10c12-CLA concentrations increased at day 84 (P = 0.007, R(2) = 0.92). Similarly, increasing dietary t10c12-CLA linearly decreased paw tumor necrosis factor (TNF)-α (P = 0.05, R(2) = 0.70). In conclusion, ≥0.125% t10c12-CLA dose-dependently reduced inflammation in a murine CA model.
authors.affiliation: Departments of Nutritional Sciences.
authors.forename: Shane M
authors.initials: SM
authors.lastname: Huebner
authors.forename: Jake M
authors.initials: JM
authors.lastname: Olson
authors.forename: James P
authors.initials: JP
authors.lastname: Campbell
authors.forename: Jeffrey W
authors.initials: JW
authors.lastname: Bishop
authors.forename: Peter M
authors.initials: PM
authors.lastname: Crump
authors.forename: Mark E
authors.initials: ME
authors.lastname: Cook
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001169:Arthritis, Experimental / Q000097:blood / Q000378:metabolism / Q000517:prevention & control*; D003094:Collagen; D004032:Diet; D004041:Dietary Fats / Q000494:pharmacology / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D053583:Interleukin-1beta / Q000097:blood*; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D011897:Random Allocation; D012720:Severity of Illness Index; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D000893:Anti-Inflammatory Agents; D004041:Dietary Fats; D053583:Interleukin-1beta; D044243:Linoleic Acids, Conjugated; D014409:Tumor Necrosis Factor-alpha; C496197:trans-10,cis-12-conjugated linoleic acid; D003094:Collagen