title: Fisetin inhibits migration, invasion and epithelial-mesenchymal transition of LMP1-positive nasopharyngeal carcinoma cells.
abstract: Fisetin (3,3',4',7-tetrahydroxyflavone) has been reported to possess certain anticancer properties. It may inhibit tumor cell proliferation, metastasis and induce apoptosis. However, the effects of fisetin in preventing the metastasis of nasopharyngeal carcinoma (NPC) cells remain to be determined. The epithelial-mesenchymal transition (EMT) is involved in several metastatic malignancies including NPC. It has been reported that the Epstein-Barr virus latent membrane protein-1 (LMP1) induced EMT and is associated with the metastasis of NPC. The aim of this study was to examine the effects of fisetin in preventing the migration and invasion of LMP1-expressing NPC cells (CNE1-LMP1 cells), as well as to investigate whether fisetin may inhibit the molecular changes associated with EMT induced by LMP1. The investigation demonstrated that fisetin suppressed the migration and invasion of CNE1-LMP1 cells under non-cytotoxic concentrations. Fisetin inhibited molecular changes associated with EMT induced by LMP1, upregulated the epithelial marker, E-cadherin protein, and downregulated the mesenchymal marker, vimentin protein, levels. Fisetin also significantly reduced the levels of Twist protein, an EMT regulator. The investigation suggested that fisetin inhibits the migration and invasion of LMP1-positive NPC cells, and the molecular mechanism involves fisetin reversing the EMT induced by LMP1 and downregulates the expression of Twist. This study indicated that fisetin serves as a potential candidate for the treatment of cancer metastasis.
authors.affiliation: Department of Pathology and Pathophysiology, Guangdong Medical College, Zhanjiang, Guangdong 524023, P.R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Pathology and Pathophysiology, Guangdong Medical College, Zhanjiang, Guangdong 524023, P.R. China.
authors.forename: Yinhai
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Pathology and Pathophysiology, Guangdong Medical College, Zhanjiang, Guangdong 524023, P.R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Pathology and Pathophysiology, Guangdong Medical College, Zhanjiang, Guangdong 524023, P.R. China.
authors.forename: Songjun
authors.initials: S
authors.lastname: Shao
authors.affiliation: Department of Physiology, Guangdong Medical College, Zhanjiang, Guangdong 524023, P.R. China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Zhang
mesh_terms: D002277:Carcinoma; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects* / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects / Q000235:genetics; D005419:Flavonoids / Q000008:administration & dosage*; D044948:Flavonols; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis / Q000188:drug therapy* / Q000473:pathology; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: C037662:EBV-associated membrane antigen, Epstein-Barr virus; D005419:Flavonoids; D044948:Flavonols; D014763:Viral Matrix Proteins; C017875:fisetin