title: Application of quantitative second-harmonic generation microscopy to dynamic conditions.
abstract: We present a quantitative second-harmonic generation (SHG) imaging technique that quantifies the 2D spatial organization of collagen fiber samples under dynamic conditions, as an image is acquired. The technique is demonstrated for both a well-aligned tendon sample and a randomly aligned, sparsely distributed collagen scaffold sample. For a fixed signal-to-noise ratio, we confirm the applicability of this method for various window sizes (pixel areas) as well as with using a gridded overlay map that allows for correlations of fiber orientations within a given image. This work has direct impact to in vivo biological studies by incorporating simultaneous SHG image acquisition and analysis.
authors.affiliation: Photonics Research of Bio/nano Environments (PROBE) Department of Mechanical Science and Engineering, University of Illinois Urbana-Champaign, 1206 W Green St, Urbana, IL 61801, USA.
authors.forename: Mohammad M
authors.initials: MM
authors.lastname: Kabir
authors.forename: V V G Krishna
authors.initials: VV
authors.lastname: Inavalli
authors.forename: Tung-Yuen
authors.initials: TY
authors.lastname: Lau
authors.forename: Kimani C
authors.initials: KC
authors.lastname: Toussaint
keywords: (100.2960) Image analysis; (180.4315) Nonlinear microscopy