title: Protective effect of fish oil supplementation on DNA damage induced by cigarette smoking.
abstract: The study examined the influence of fish oil (FO) supplementation on serum 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels as indicated by DNA damage markers and total antioxidant capacity (TAC) among male cigarette smokers. This double-blind, placebo-controlled randomized study was conducted among healthy cigarette smokers (n=40) who were part of a larger prospective cohort study. Twenty smokers were randomly selected to receive FO for 3 months (1 g/day), and another 20 smokers received a placebo for 3 months; 8-OHdG and TAC levels were measured in blood samples before and after the intervention. Serum 8-OHdG significantly decreased (p=0.001) and TAC increased (p<0.001) after 3 months of treatment with FO. Between baseline and endline, the difference in 8-OHdG significantly correlated with the difference in TAC among smokers who received FO (r=-0.540, p=0.014). The study provides evidence that FO supplementation can modify decreased antioxidants and increased oxidative DNA damage in cigarette smokers.
authors.affiliation: Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Ghorbanihaghjo
authors.forename: Javid
authors.initials: J
authors.lastname: Safa
authors.forename: Samira
authors.initials: S
authors.lastname: Alizadeh
authors.forename: Hassan
authors.initials: H
authors.lastname: Argani
authors.forename: Nadereh
authors.initials: N
authors.lastname: Rashtchizadeh
authors.forename: Mohammad Vahid
authors.initials: MV
authors.lastname: Taghinia
authors.forename: Mehran Mesgari
authors.initials: MM
authors.lastname: Abbasi
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood; D015331:Cohort Studies; D004249:DNA Damage / Q000187:drug effects*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D019587:Dietary Supplements; D004311:Double-Blind Method; D005395:Fish Oils / Q000494:pharmacology*; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D011446:Prospective Studies; D012907:Smoking / Q000097:blood*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D005395:Fish Oils; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine