title: Rapid method for the simultaneous determination of flavonol aglycones in food using u-HPLC coupled with heating block acidic hydrolysis.
abstract: A rapid method for the simultaneous determination of flavonol aglycones in food using ultra-high-performance LC (u-HPLC) coupled with a heating-block acidic hydrolysis method was validated in terms of precision, accuracy, and linearity. The u-HPLC separation was performed on an RP C18 column (particle size 2 micro m id, 2 mm, length 100 mm) with a photodiode array detector. The LOD and LOQ of the u-HPLC analyses were 0.15 and 0.47 mg/kg for myricetin, 0.09 and 0.28 mg/kg for quercetin, 0.16 and 0.49 mg/kg for kaempferol, and 0.08 and 0.25 mg/kg for isorhamnetin. The intraday and interday precisions of the individual flavonol aglycones were less than 9.31%. All calibration curves exhibited good linearity (r2 = 0.99) within the tested ranges. Total run time of u-HPLC was 13 min. The rapid u-HPLC method presented herein significantly improved the speed, sensitivity, and resolution of the analyses of myricetin, quercetin, kaempferol, and isorhamnetin in food.
authors.affiliation: Korea Food Research Institute, Food Analysis Center, 516, Baekhyeon, Bundang, Seongnam, Gyeonggi, 463-746, Republic of Korea.
authors.forename: You-Shin
authors.initials: YS
authors.lastname: Shim
authors.forename: Seunghee
authors.initials: S
authors.lastname: Kim
authors.forename: Dongwon
authors.initials: D
authors.lastname: Seo
authors.forename: Masahito
authors.initials: M
authors.lastname: Ito
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nakagawa
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Park
authors.forename: Jaeho
authors.initials: J
authors.lastname: Ha
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis; D044948:Flavonols / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D006361:Heating; D006868:Hydrolysis; D044949:Kaempferols / Q000032:analysis; D057230:Limit of Detection; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis; D013997:Time Factors
chemical_list: D005419:Flavonoids; D044948:Flavonols; D044949:Kaempferols; C047368:3-methylquercetin; C006552:kaempferol; C040015:myricetin; D011794:Quercetin