title: Changes in pulmonary tissue structure and KL-6/MUC1 expression in a newborn rat model of hyperoxia-induced bronchopulmonary dysplasia.
abstract: Following preterm birth, levels of Krebs von den Lungen-6/mucin 1 (KL-6/MUC1) in serum correlate closely with the development of advanced bronchopulmonary dysplasia (BPD), but the role of KL-6/MUC1 in the development of BPD is unclear. To explore whether a relationship exists between KL-6/MUC1 and pathological changes in BPD and verify such a clinical finding, we established a newborn rat model of 95% oxygen-induced BPD. The development of pulmonary alveoli was evaluated by determining the radial alveolar count (RAC) and examining the location, distribution, and expression of KL-6/MUC1 in pulmonary tissues using a fluorescent immunoassay, Western blot, and reverse transcription polymerase chain reaction. The synchronic expression levels of KL-6/MUC1 in serum, bronchoalveolar lavage fluid (BALF) and pulmonary tissues were examined using an enzyme-linked immunosorbent assay. The mean RAC in the hyperoxia group was significantly lower than in normoxia controls, whereas the expression levels of KL-6/MUC1 were higher. On days 1, 3, 7, and 14, the mean RACs in hyperoxic rats were 15.00, 12.67, 12.00, and 11.33, respectively. The expression levels of KL-6/MUC1 peaked in the experimental group on day 1, and began to decrease slightly after day 3. The expression levels of KL-6/MUC1 in serum and BALF were associated with KL-6/MUC1 expression in pulmonary tissues. We suggest that increased lung KL-6/MUC1 expression appears to be closely associated with impairment of alveolarization in a newborn rat model of hyperoxia-induced BPD. Changes in lung KL-6/MUC1 expression can be evaluated effectively and less invasively by monitoring KL-6/MUC1 in serum and BALF.
authors.affiliation: Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Zhu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Fu
authors.forename: Kai
authors.initials: K
authors.lastname: You
authors.forename: Linlin
authors.initials: L
authors.lastname: Jin
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Danchi
authors.initials: D
authors.lastname: Lu
authors.forename: Xindong
authors.initials: X
authors.lastname: Xue
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D001997:Bronchopulmonary Dysplasia / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D015870:Gene Expression; D018496:Hyperoxia / Q000150:complications*; D008168:Lung / Q000378:metabolism* / Q000473:pathology*; D018396:Mucin-1 / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
chemical_list: C506177:MUC1 protein, rat; D018396:Mucin-1; D012333:RNA, Messenger