title: Scalable transient protein expression.
abstract: Transient transfection is a well-established method to rapidly express recombinant proteins from mammalian cells. Accelerating activity in biotherapeutic drug development, demand for protein-based reagents, vaccine research, and large initiatives in structural and functional studies of proteins have propelled the need to generate moderate to high amounts of recombinant proteins and other macromolecules in a flexible and rapid manner. Progress over the last 10-15 years has demonstrated that transient transfections can be reliably and readily scaled up to handle milliliters to tens of liters of cells in suspension culture and obtain milligrams to grams of recombinant protein in a process that requires only days to weeks. This review will summarize developments in this field, properties of the components of a transient expression system that enable maximal protein production, and detailed protocols for this application.
authors.affiliation: Life Technologies, Carlsbad, CA, USA.
authors.forename: Henry C
authors.initials: HC
authors.lastname: Chiou
authors.forename: Sanjay
authors.initials: S
authors.lastname: Vasu
authors.forename: Chao Yan
authors.initials: CY
authors.lastname: Liu
authors.forename: Isabel
authors.initials: I
authors.lastname: Cisneros
authors.forename: Meredith B
authors.initials: MB
authors.lastname: Jones
authors.forename: Jonathan F
authors.initials: JF
authors.lastname: Zmuda
mesh_terms: D000818:Animals; D016466:CHO Cells; D018929:Cell Culture Techniques; D003412:Cricetulus; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D011994:Recombinant Proteins