title: Effects of pH and fermentation strategies on 2,3-butanediol production with an isolated Klebsiella sp. Zmd30 strain.
abstract: This study examined the effects of pH-controlled and fermentation strategies on 2,3-butanediol (2,3-BDO) production by an isolated Klebsiella sp. Zmd30 strain. The pH value of 6.0 was found to be optimal among the investigated range of 4.5-9.0. In batch fermentation, the concentration, productivity and yield of 2,3-BDO were 57.17 g/l, 1.59 g/l/h and 82%, respectively. The 2,3-BDO production by Klebsiella sp. Zmd30 was found to be growth-associated. Higher 2,3-BDO concentration (110 g/l) and yield (94%) was obtained by using fed-batch operation, but the productivity was lower (0.88 g/l/h) as compared to that when using batch operation. The highest 2,3-BDO productivity of 2.81 g/l/h was obtained with the continuous culture at a hydraulic retention time (HRT) of 12h. The results suggest that fed-batch operation might be most suitable for commercialized 2,3-BDO production, due to obtaining a high concentration and yield.
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Chiao-Ling
authors.initials: CL
authors.lastname: Wong
authors.affiliation: Department of Chemical and Materials Engineering, Tunghai University, Taichung 407, Taiwan. Electronic address: hwyen@thu.edu.tw.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Yen
authors.affiliation: Solvent and Chemical Business Division, CPC Corporation, Taiwan, Chia-Yi 600, Taiwan.
authors.forename: Chung-Liang
authors.initials: CL
authors.lastname: Lin
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan; Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 701, Taiwan; University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan. Electronic address: changjs@mail.ncku.edu.tw.
authors.forename: Jo-Shu
authors.initials: JS
authors.lastname: Chang
mesh_terms: D001419:Bacteria / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D001709:Biotechnology / Q000379:methods*; D002072:Butylene Glycols / Q000378:metabolism*; D004583:Electrons; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007709:Klebsiella / Q000302:isolation & purification* / Q000378:metabolism*; D013997:Time Factors
chemical_list: D002072:Butylene Glycols; C026978:2,3-butylene glycol
keywords: 2,3-Butanediol; Fermentation mode; Klebsiella sp.; pH control