title: Microrheology of keratin networks in cancer cells.
abstract: Microrheology is a valuable tool to determine viscoelastic properties of polymer networks. For this purpose measurements with embedded tracer beads inside the extracted network of pancreatic cancer cells were performed. Observing the beads motion with a CCD-high-speed-camera leads to the dynamic shear modulus. The complex shear modulus is divided into real and imaginary parts which give insight into the mechanical properties of the cell. The dependency on the distance of the embedded beads to the rim of the nucleus shows a tendency for a decreasing storage modulus. We draw conclusions on the network topology of the keratin network types based on the mechanical behavior.
authors.affiliation: Department for Experimental Physics, University of Ulm, D-89069 Ulm, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Paust
authors.forename: S
authors.initials: S
authors.lastname: Paschke
authors.forename: M
authors.initials: M
authors.lastname: Beil
authors.forename: O
authors.initials: O
authors.lastname: Marti
mesh_terms: D001696:Biomechanical Phenomena; D045744:Cell Line, Tumor; D004548:Elasticity; D006801:Humans; D007633:Keratins / Q000032:analysis*; D010179:Pancreas / Q000737:chemistry / Q000166:cytology / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000737:chemistry* / Q000473:pathology*; D012212:Rheology; D014783:Viscosity
chemical_list: D007633:Keratins