title: Photodynamic diagnosis of colitis-associated dysplasia in a mouse model after oral administration of 5-aminolevulinic acid.
abstract: BACKGROUND
  Detection of dysplastic lesions in mouse colonic tissue was investigated by accumulation of photosensitizer protoporphyrin IX (PpIX) induced by oral administration of 5-aminolevulinic acid (5-ALA), a precursor of PplX.
  
  
  METHODS
  Inflammatory oncogenesis was induced in C57BL/6J-Apc(Min) (Apc(Min/+)) mouse by oral administration of dextran sodium sulfate (DSS). After oral administration of 5-ALA, the colonic tissue was observed by autofluorescent stereoscopy and histopathological examination. The localization of fluorescence signals in the colonic tissue was determined with a mobile ultraviolet Xe lamp light.
  
  
  RESULTS
  Several small polypoid lesions were found in the mucosal layer of DSS-treated Apc(Min/+) mice. Strong reddish ring-shaped fluorescence signals of PpIX, at 635 nm measured by a spectrum analyzer, were observed on the mucosal surface of all protruding lesions that were confirmed to be histopathologically dysplastic.
  
  
  CONCLUSIONS
  Photodynamic diagnosis with 5-ALA was useful in detecting dysplastic lesions in the colonic mucosa in a mouse model.
authors.affiliation: Department of Endoscopy, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan. tkato@jikei.ac.jp.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Komoike
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kato
authors.forename: Hiroki
authors.initials: H
authors.lastname: Saijo
authors.forename: Seiji
authors.initials: S
authors.lastname: Arihiro
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Masataka
authors.initials: M
authors.lastname: Okabe
authors.forename: Masaki
authors.initials: M
authors.lastname: Ito
authors.forename: Shigeo
authors.initials: S
authors.lastname: Koido
authors.forename: Sadamu
authors.initials: S
authors.lastname: Homma
authors.forename: Hisao
authors.initials: H
authors.lastname: Tajiri
mesh_terms: D000622:Aminolevulinic Acid / Q000378:metabolism / Q000493:pharmacokinetics; D000818:Animals; D003092:Colitis / Q000175:diagnosis* / Q000378:metabolism; D003106:Colon / Q000378:metabolism / Q000473:pathology; D016264:Dextran Sulfate; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D061848:Optical Imaging; D017319:Photosensitizing Agents / Q000378:metabolism / Q000493:pharmacokinetics; D011524:Protoporphyrins / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D017319:Photosensitizing Agents; D011524:Protoporphyrins; D000622:Aminolevulinic Acid; D016264:Dextran Sulfate; C028025:protoporphyrin IX
keywords: 5-aminolevulinic acid; Photodynamic diagnosis; colitis-associated dysplasia; ulcerative colitis