title: Effect of silanization on chitosan porous scaffolds for peripheral nerve regeneration.
abstract: The aim of this study was to evaluate the feasibility of using 3-aminopropyltriethoxysilane (APTE) silanization treatment for modification and biocompatibility of lyophilized chitosan porous scaffolds. The process is beneficial for biomaterial development due to its low toxicity and simplicity. The silanization treatment with low APTE concentration showed no significant influence on the morphology of chitosan scaffolds, while a skin-like surface was observed for the silanized scaffolds treated with high APTE concentration. The porosity and surface amino densities were increased after silanization whereas the swelling ratio was reduced, and the degradation ratio in PBS and anti-acid degradation properties of the silanized chitosan scaffolds were significantly improved. The in vitro Schwann cells culture demonstrated that the silanized scaffolds with 8% APTE could obviously facilitate the attachment and proliferation of Schwann cells, indicating great potential for the application in peripheral nerve regeneration.
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, PR China.
authors.forename: Guicai
authors.initials: G
authors.lastname: Li
authors.forename: Luzhong
authors.initials: L
authors.lastname: Zhang
authors.forename: Caiping
authors.initials: C
authors.lastname: Wang
authors.forename: Xueying
authors.initials: X
authors.lastname: Zhao
authors.forename: Changlai
authors.initials: C
authors.lastname: Zhu
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Zheng
authors.forename: Yaling
authors.initials: Y
authors.lastname: Wang
authors.forename: Yahong
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yumin
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000818:Animals; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D005240:Feasibility Studies; D005612:Freeze Drying; D009416:Nerve Regeneration / Q000187:drug effects*; D010525:Peripheral Nerves / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D016062:Porosity; D011437:Propylamines; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects; D012821:Silanes / Q000737:chemistry*; D013329:Structure-Activity Relationship; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011437:Propylamines; D012821:Silanes; D048271:Chitosan; C477625:amino-propyl-triethoxysilane
keywords: Chitosan porous scaffolds; Peripheral nerve regeneration; Schwann cells; Silanization