title: Proteomics study of silver nanoparticles toxicity on Bacillus thuringiensis.
abstract: Emerging technologies in functional genomics and proteomics provide a way of achieving high-throughput analyses, understanding effects on protein populations and sub-populations and follow up environmental stresses. To accomplish these, the action of homemade spherical Silver nanoparticles colloidal suspension (AgNPs) against Bacillus thuringiensis (isolate from Oryza sativa L. rhizosphere) was investigated by a proteomic approach (2-DE and NanoLC/FT-ICR MS identification). Thirty four responsive (up/down regulated) proteins were identified. Proteomic results revealed that an exposure of B. thuringiensis cells with different concentrations of AgNPs resulted in an accumulation of envelope protein precursors, indicative of the dissipation of a proton motive force. Identified proteins are involved in oxidative stress tolerance, metal detoxification, transcription and elongation processes, protein degradation, cytoskeleton remodeling and cell division. The expression pattern of these proteins and their possible involvement in the nontoxicity mechanisms were discussed.
authors.affiliation: Department of Biotechnology, The Faculty of Renewable Energies & New Technologies Engineering (NTE), Shahid Beheshti University, G.C. Evin, Tehran, Iran; Department of Nanobiotechnology, Protein Research Institute, Shahid Beheshti University, G.C. Evin, Tehran, Iran. Electronic address: F_mirzajani@scientist.com.
authors.forename: Fateme
authors.initials: F
authors.lastname: Mirzajani
authors.affiliation: Department of Biotechnology, The Faculty of Renewable Energies & New Technologies Engineering (NTE), Shahid Beheshti University, G.C. Evin, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Askari
authors.affiliation: Department of Biotechnology, The Faculty of Renewable Energies & New Technologies Engineering (NTE), Shahid Beheshti University, G.C. Evin, Tehran, Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Hamzelou
authors.affiliation: Institute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, Schubertstrasse 60, 35392 Giessen, Germany.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Schober
authors.affiliation: Institute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, Schubertstrasse 60, 35392 Giessen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Römpp
authors.affiliation: Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C. Evin, Tehran, Iran. Electronic address: aghassempour@scientist.com.
authors.forename: Alireza
authors.initials: A
authors.lastname: Ghassempour
authors.affiliation: Institute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, Schubertstrasse 60, 35392 Giessen, Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Spengler
mesh_terms: D001413:Bacillus thuringiensis / Q000187:drug effects*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D053758:Nanoparticles / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D020543:Proteome / Q000187:drug effects*; D040901:Proteomics; D012834:Silver / Q000633:toxicity*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D012834:Silver
keywords: Bacillus thuringiensis; Metal detoxification; NanoLC/FT-ICR; Oxidative stress; Silver nanoparticle; Two dimensional electrophoresis