title: Sensory-neuron subtype-specific transcriptional programs controlling dendrite morphogenesis: genome-wide analysis of Abrupt and Knot/Collier.
abstract: The transcription factors Abrupt (Ab) and Knot (Kn) act as selectors of distinct dendritic arbor morphologies in two classes of Drosophila sensory neurons, termed class I and class IV, respectively. We performed binding-site mapping and transcriptional profiling of these isolated neurons. Their profiles were similarly enriched in cell-type-specific enhancers of genes implicated in neural development. We identified a total of 429 target genes, of which 56 were common to Ab and Kn; these targets included genes necessary to shape dendritic arbors in either or both of the two sensory subtypes. Furthermore, a common target gene, encoding the cell adhesion molecule Ten-m, was expressed more strongly in class I than class IV, and this differential was critical to the class-selective directional control of dendritic branch sprouting or extension. Our analyses illustrate how differentiating neurons employ distinct and shared repertoires of gene expression to produce class-selective morphological traits.
authors.affiliation: Graduate School of Biostudies, Kyoto University, Kyoto 606-8501, Japan.
authors.forename: Yukako
authors.initials: Y
authors.lastname: Hattori
authors.affiliation: Graduate School of Biostudies, Kyoto University, Kyoto 606-8501, Japan.
authors.forename: Tadao
authors.initials: T
authors.lastname: Usui
authors.affiliation: Graduate School of Biostudies, Kyoto University, Kyoto 606-8501, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Satoh
authors.affiliation: Kobayashi-Maskawa Institute, Nagoya University, Aichi 464-8602, Japan; Graduate School of Mathematics, Nagoya University, Aichi 464-8602, Japan.
authors.forename: Sanefumi
authors.initials: S
authors.lastname: Moriyama
authors.affiliation: Graduate School of Biostudies, Kyoto University, Kyoto 606-8501, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Shimono
authors.affiliation: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Kanagawa 226-8501, Japan.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Itoh
authors.affiliation: Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 113-0032, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Shirahige
authors.affiliation: Graduate School of Biostudies, Kyoto University, Kyoto 606-8501, Japan. Electronic address: tauemura@lif.kyoto-u.ac.jp.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Uemura
mesh_terms: D000818:Animals; D002843:Chromatin / Q000235:genetics; D003712:Dendrites / Q000502:physiology; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D055106:Genome-Wide Association Study; D007814:Larva / Q000166:cytology / Q000502:physiology; D009024:Morphogenesis / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000502:physiology* / Q000648:ultrastructure; D019063:Tenascin / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000502:physiology*; D059467:Transcriptome
chemical_list: D002843:Chromatin; D029721:Drosophila Proteins; D009687:Nuclear Proteins; C089140:Ten-m protein, Drosophila; D019063:Tenascin; D014157:Transcription Factors; C531978:ab protein, Drosophila; C104357:kn protein, Drosophila