title: Ontogenetic manganese exposure with perinatal 6-OHDA lesioning alters behavioral responses of rats to dopamine D₁ and D2 agonist treatments.
abstract: The effect of neonatal manganese (Mn) exposure in a 6-hydroxydopamine (6-OHDA) rat model of Parkinson's disease was investigated. Pregnant Wistar rats were given drinking water with 10,000 ppm of Manganese (MnCl₂.4H₂O) from the time of conception until weaning on the 21st day after delivery. Control rats consumed tap water. Three days after the birth, other groups of neonatal rat pups were pretreated with desipramine (20 mg/kg ip 1h) prior to bilateral ICV administration of 6-OHDA or its vehicle, saline-ascorbic (0.1%) (control). Two months after the birth, striatal dopamine and homovanilic acid efflux measured by an in vivo microdialysis method were reduced in rats lesioned with 6-OHDA. Co-exposure to perinatal Mn did not modify neurotransmission alterations. However, there were prominent abnormalities in behavioral testing in rats perinatally exposed to Mn and treated neonatally with 6-OHDA. These findings demonstrate that although Mn did not further damage neurotransmitter activity in the neostriatum, ontogenetic exposure to Mn enhances the behavioral toxicity to 6-OHDA.
authors.affiliation: Department of Basic Medical Sciences, Medical University of Silesia, Piekarska 18, 41-902 Bytom, Poland.
authors.forename: Ryszard
authors.initials: R
authors.lastname: Szkilnik
authors.affiliation: Department of Nurse, High School of Strategic Planning, Koscielna 6, 41-303 Dabrowa Gornicza, Poland.
authors.forename: Ryszard
authors.initials: R
authors.lastname: Brus
authors.affiliation: Department of Toxicology and Occupational Health Protection, Public Health Faculty, Medical University of Silesia, Medykow 18, 40-752 Katowice Ligota, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Malinowska-Borowska
authors.affiliation: Department of Basic Medical Sciences, Medical University of Silesia, Piekarska 18, 41-902 Bytom, Poland.
authors.forename: Damian
authors.initials: D
authors.lastname: Nowak
authors.affiliation: Department of Basic Medical Sciences, Medical University of Silesia, Piekarska 18, 41-902 Bytom, Poland.
authors.forename: Martyna
authors.initials: M
authors.lastname: Waliczek
authors.affiliation: Department of Pharmacology, Quillen College of Medicine, East Tennessee State University, P.O. Box 70577, Johnson City, TN 37614, USA.
authors.forename: Richard M
authors.initials: RM
authors.lastname: Kostrzewa
authors.affiliation: Department of Toxicology and Occupational Health Protection, Public Health Faculty, Medical University of Silesia, Medykow 18, 40-752 Katowice Ligota, Poland. Electronic address: pnowak@sum.edu.pl.
authors.forename: Przemyslaw
authors.initials: P
authors.lastname: Nowak
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D003891:Desipramine / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism; D018491:Dopamine Agonists / Q000494:pharmacology*; D005106:Exploratory Behavior / Q000187:drug effects; D005260:Female; D006719:Homovanillic Acid / Q000378:metabolism; D008345:Manganese / Q000633:toxicity*; D008431:Maternal-Fetal Exchange; D009043:Motor Activity / Q000187:drug effects; D017072:Neostriatum / Q000187:drug effects / Q000378:metabolism; D016627:Oxidopamine; D010293:Pargyline / Q000494:pharmacology; D010300:Parkinson Disease / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D017447:Receptors, Dopamine D1 / Q000819:agonists; D017448:Receptors, Dopamine D2 / Q000819:agonists
chemical_list: D018491:Dopamine Agonists; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D008345:Manganese; D016627:Oxidopamine; D010293:Pargyline; D003891:Desipramine; D004298:Dopamine; D006719:Homovanillic Acid
keywords: Behavior; Brain; Exposure; Manganese; Microdialysis; Ontogenetic