title: Protective effect of iridoid glycosides from Paederia scandens (LOUR.) MERRILL (Rubiaceae) on uric acid nephropathy rats induced by yeast and potassium oxonate.
abstract: Iridoid glycosides of Paederia scandens (IGPS) are an active component isolated from Chinese herb P. scandens (LOUR.) MERRILL (Rubiaceae). Uric acid nephropathy (UAN) is caused by excessive uric acid, which results in damage of kidney tissue via urate crystals deposition in the kidneys. This study aimed to investigate the protective effects of IGPS on UAN in rats induced by yeast and potassium oxonate. Treatment groups received different doses of IGPS and allopurinol (AP) daily for 35 days respectively. The results showed that treatment with IGPS significantly prevented the increases of uric acid in serum and the elevation of systolic blood pressure (SBP), attenuated renal tissue injury, improved renal function and reserved the biological activity of NOS-1. IGPS also inhibited the biological activity of TNF-α and TGF-β1, and suppressed the mRNA expressions of TNF-α and TGF-β1 in renal tissue. Taken together, the present and our previous findings suggest that IGPS exerts protective effects against kidney damage in UAN rats through its uric acid-lowering, anti-inflammatory and immunomodulatory properties. Furthermore, decreasing SBP by up regulation of NOS-1 expression and down regulation of TNF-α and TGF-β1 expression are involved in the effect of IGPS on high uric acid-induced nephropathy.
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Anhui Medical University, Hefei, Anhui 230032, China.
authors.forename: Shi-xiang
authors.initials: SX
authors.lastname: Hou
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Anhui Medical University, Hefei, Anhui 230032, China; Pharmacy Intravenous Admixture Service, Hefei Binhu Hospital, Hefei 230601, China.
authors.forename: Wen-jing
authors.initials: WJ
authors.lastname: Zhu
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Anhui Medical University, Hefei, Anhui 230032, China.
authors.forename: Ming-qun
authors.initials: MQ
authors.lastname: Pang
authors.affiliation: Department of Anesthesiology, Washington University, School of Medicine, St. Louis, MO 63110, USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Jeffry
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Anhui Medical University, Hefei, Anhui 230032, China. Electronic address: lanlzhou@aliyun.com.
authors.forename: Lan-lan
authors.initials: LL
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001483:Base Sequence; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D017931:DNA Primers; D004195:Disease Models, Animal; D006973:Hypertension / Q000150:complications; D057889:Iridoid Glycosides / Q000494:pharmacology*; D007674:Kidney Diseases / Q000097:blood / Q000209:etiology / Q000517:prevention & control*; D008297:Male; D019001:Nitric Oxide Synthase / Q000378:metabolism; D010094:Oxonic Acid / Q000633:toxicity*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019666:Rubiaceae / Q000737:chemistry*; D053773:Transforming Growth Factor beta1 / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D014527:Uric Acid / Q000097:blood*; D015003:Yeasts
chemical_list: D017931:DNA Primers; D057889:Iridoid Glycosides; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D014527:Uric Acid; C489337:potassium oxonate; D010094:Oxonic Acid; D003404:Creatinine; D019001:Nitric Oxide Synthase
keywords: Hypertension; Iridoid glycosides of Paederia scandens; NOS-1; TGF-β1; TNF-α; Uric acid nephropathy