title: Blocked bacteria escape by ATRP grafting of a PMMA shell on PVA microparticles.
abstract: This paper reports on the preparation and characterization of living composites consisting of poly(vinylalcohol) (PVA) hydrogel microparticles with living bacteria and a shell of poly(methyl methacrylate) (PMMA). The grafting of the PMMA shell is accomplished in the presence of living bacteria by surface polymerization of PMMA using atom transfer radical polymerization (ATRP). The PMMA shell prevents the uncontrolled bacterial escape from the hydrogel microparticles, which otherwise marks a major problem of these composites. The encapsulation of microparticles with living bacteria by PMMA retards bacteria escape upon contact to water for >20 d. The functionality of the PMMA shell is proven both by the release of fluorescein in buffer and an altered release time of bacteria in buffer solution.
authors.affiliation: Department of Chemistry and Center of Materials Science, Philipps-Universität Marburg, Hans-Meerwein-Straße, D-35042, Marburg, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Knierim
authors.forename: Charles L
authors.initials: CL
authors.lastname: Greenblatt
authors.forename: Seema
authors.initials: S
authors.lastname: Agarwal
authors.forename: Andreas
authors.initials: A
authors.lastname: Greiner
mesh_terms: D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry; D058105:Polymerization; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011142:Polyvinyl Alcohol; D019904:Polymethyl Methacrylate
keywords: bacteria; biohybrids; composites; core-shell polymers; hydrogels