title: Factors influencing the incidence of habituation for cytokinin of tobacco pith tissue in culture.
abstract: Pith tissue of Nicotiana tabacum L. cv. "Havana 425" exhibits a gradient in its tendency to habituate for cytokinin on an auxin-containing medium at 35° C, about 10° C above the standard culture temperature. Explants of pith from below the 8th to 11th internode, counting from the bottom of the plant, rarely habituate for cytokinin; explants from above this threshold habituate rapidly. The explants must also be above a critical size, about 20-30 mg, to habituate. There was a pronounced interaction between size and position effects; the threshold position for cytokinin habituation shifted upward with decreasing explant size.
authors.affiliation: Department of Botany, University of Illinois, 61801, Urbana, IL, USA.
authors.forename: F
authors.initials: F
authors.lastname: Meins
authors.forename: J
authors.initials: J
authors.lastname: Lutz
authors.forename: R
authors.initials: R
authors.lastname: Foster