title: High-throughput synchronization of mammalian cell cultures by spiral microfluidics.
abstract: The development of mammalian cell cycle synchronization techniques has greatly advanced our understanding of many cellular regulatory events and mechanisms specific to different phases of the cell cycle. In this chapter, we describe a high-throughput microfluidic-based approach for cell cycle synchronization. By exploiting the relationship between cell size and its phase in the cell cycle, large numbers of synchronized cells can be obtained by size fractionation in a spiral microfluidic channel. Protocols for the synchronization of primary cells such as mesenchymal stem cells, and immortal cell lines such as Chinese hamster ovarian cells (CHO-CD36) and HeLa cells are provided as examples.
authors.affiliation: NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Singapore.
authors.forename: Wong Cheng
authors.initials: WC
authors.lastname: Lee
authors.forename: Ali Asgar S
authors.initials: AA
authors.lastname: Bhagat
authors.forename: Chwee Teck
authors.initials: CT
authors.lastname: Lim
mesh_terms: D000818:Animals; D016466:CHO Cells; D018929:Cell Culture Techniques / Q000379:methods*; D002453:Cell Cycle; D048429:Cell Size; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000032:analysis; D004867:Equipment Design; D005434:Flow Cytometry; D006367:HeLa Cells; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D059630:Mesenchymal Stem Cells; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*
chemical_list: D004247:DNA