title: Proteomic analysis of outer membrane vesicles from the probiotic strain Escherichia coli Nissle 1917.
abstract: Escherichia coli Nissle 1917 (EcN) is a probiotic used for the treatment of intestinal disorders. EcN improves gastrointestinal homeostasis and microbiota balance; however, little is known about how this probiotic delivers effector molecules to the host. Outer membrane vesicles (OMVs) are constitutively produced by Gram-negative bacteria and have a relevant role in bacteria-host interactions. Using 1D SDS-PAGE and highly sensitive LC-MS/MS analysis we identified in this study 192 EcN vesicular proteins with high confidence in three independent biological replicates. Of these proteins, 18 were encoded by strain-linked genes and 57 were common to pathogen-derived OMVs. These proteins may contribute to the ability of this probiotic to colonize the human gut as they fulfil functions related to adhesion, immune modulation or bacterial survival in host niches. This study describes the first global OMV proteome of a probiotic strain and provides evidence that probiotic-derived OMVs contain proteins that can target these vesicles to the host and mediate their beneficial effects on intestinal function. All MS data have been deposited in the ProteomeXchange with identifier PXD000367 (http://proteomecentral.proteomexchange.org/dataset/PXD000367).
authors.affiliation: Departament de Bioquímica i Biologia Molecular, Facultat de Farmàcia, Institut de Biomedicina de la Universitat de Barcelona (IBUB), Universitat de Barcelona, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Aguilera
authors.forename: Lorena
authors.initials: L
authors.lastname: Toloza
authors.forename: Rosa
authors.initials: R
authors.lastname: Giménez
authors.forename: Antonia
authors.initials: A
authors.lastname: Odena
authors.forename: Eliandre
authors.initials: E
authors.lastname: Oliveira
authors.forename: Juan
authors.initials: J
authors.lastname: Aguilar
authors.forename: Josefa
authors.initials: J
authors.lastname: Badia
authors.forename: Laura
authors.initials: L
authors.lastname: Baldomà
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry* / Q000166:cytology; D029968:Escherichia coli Proteins / Q000032:analysis*; D019936:Probiotics / Q000737:chemistry*; D040901:Proteomics; D053719:Tandem Mass Spectrometry
chemical_list: D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins
keywords: Escherichia coli Nissle 1917; Microbiology; Outer membrane vesicles; Probiotics; Proteome