title: Breed differences in insulin sensitivity and insulinemic responses to oral glucose in horses and ponies of moderate body condition score.
abstract: Breed-related differences may occur in the innate insulin sensitivity (SI) of horses and ponies, an important factor believed to be associated with the risk of laminitis. The aim of this study was to measure the glucose and insulin responses of different breeds of horses and ponies in moderate body condition to a glucose-containing meal and to compare these responses with the indices of SI as determined by a frequently sampled intravenous glucose tolerance test (FSIGT). Eight Standardbred horses, 8 mixed-breed ponies, and 7 Andalusian-cross horses with a mean ± SEM BCS 5.0 ± 0.3 of 9 were used in this study. Each animal underwent an oral glucose tolerance test (OGTT) in which they were fed a fiber-based ration (2.0 g/kg BW) containing 1.5 g/kg BW added glucose, as well as a standard FSIGT with minimal model analysis. The glucose response variables from the OGTT were similar between groups; however, the peak insulin concentration was higher in ponies (94.1 ± 29.1 μIU/mL; P = 0.003) and Andalusians (85.3 ± 18.6; P = 0.004) than in Standardbreds (21.2 ± 3.5). The insulin area under the curve was also higher in ponies (13.5 ± 3.6 IU · min · L(-1); P = 0.009) and Andalusians (15.0 ± 2.7; P = 0.004) than in Standardbreds (3.1 ± 0.6). Insulin sensitivity, as determined by the FSIGT, was lower in Andalusians (0.99 ± 0.18 × 10(-4)/[mIU · min]) than in Standardbreds (5.43 ± 0.94; P < 0.001) and in ponies (2.12 ± 0.44; P = 0.003) than in Standardbreds. Peak insulin concentrations from the OGTT were negatively correlated with SI (P < 0.001; rs = -0.75). These results indicate that there are clear breed-related differences in the insulin responses of horses and ponies to oral and intravenous glucose. All animals were in moderate body condition, indicating that breed-related differences in insulin dynamics occurred independent of obesity.
authors.affiliation: Faculty of Veterinary Science, The University of Melbourne, Werribee, Victoria 3030, Australia.
authors.forename: N J
authors.initials: NJ
authors.lastname: Bamford
authors.affiliation: Faculty of Veterinary Science, The University of Melbourne, Werribee, Victoria 3030, Australia.
authors.forename: S J
authors.initials: SJ
authors.lastname: Potter
authors.affiliation: Equine Studies Group, WALTHAM Centre for Pet Nutrition, Melton Mowbray, LE14 4RT, UK.
authors.forename: P A
authors.initials: PA
authors.lastname: Harris
authors.affiliation: Faculty of Veterinary Science, The University of Melbourne, Werribee, Victoria 3030, Australia. Electronic address: bais@unimelb.edu.au.
authors.forename: S R
authors.initials: SR
authors.lastname: Bailey
mesh_terms: D000818:Animals; D001823:Body Composition / Q000502:physiology*; D005951:Glucose Tolerance Test / Q000662:veterinary; D006736:Horses / Q000097:blood* / Q000235:genetics / Q000502:physiology*; D007328:Insulin / Q000502:physiology*; D007333:Insulin Resistance / Q000502:physiology*
chemical_list: D007328:Insulin
keywords: Equine; Glucose; Hyperinsulinemia; Insulin resistance; Laminitis; Obesity