title: Glioblastoma-specific anticancer activity of pheophorbide a from the edible red seaweed Grateloupia elliptica.
abstract: The chlorophyll-related compound pheophorbide a (Pa) was successively purified from an edible red seaweed, Grateloupia elliptica, using silica, octadecyl silica column chromatography and reversed phase-high-performance liquid chromatography, as well as the cell cycle inhibitory and apoptotic effects of Pa being investigated in U87MG glioblastoma cells. The Pa exhibited strong anticancer effects in the absence of direct photo-irradiation against various cancer cell lines, including U87MG, SK-OV-3, and HeLa cells. Among the cancer cells, the strongest anticancer activity of Pa exhibited on U87MG cells with IC50 values of 2.8 μg/ml. In addition, Pa specifically had cytostatic activity on glioblastoma cells rather than human umbilical vein endothelial cells. Analysis of the cell cycle distribution showed that Pa induced G0/G1 arrest of U87 MG cells. In addition, arrested cells induced late apoptosis and DNA degradation under dark condition. These results suggest that Pa isolated from G. elliptica is a potential glioblastoma-specific anticancer agent without side effects on normal cells.
authors.affiliation: East Sea Research Institute, Korea Institute of Ocean Science and Technology, Gyeongbuk, 767-813, Republic of Korea.
authors.forename: Myounglae
authors.initials: M
authors.lastname: Cho
authors.forename: Gab-Man
authors.initials: GM
authors.lastname: Park
authors.forename: Su-Nam
authors.initials: SN
authors.lastname: Kim
authors.forename: Touseef
authors.initials: T
authors.lastname: Amna
authors.forename: Seokjoon
authors.initials: S
authors.lastname: Lee
authors.forename: Woon-Seob
authors.initials: WS
authors.lastname: Shin
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D017209:Apoptosis; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002734:Chlorophyll / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D054697:Cytostatic Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D053938:DNA Fragmentation; D042783:Endothelial Cells / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D009474:Neurons / Q000187:drug effects*; D000461:Rhodophyta / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D054697:Cytostatic Agents; D002734:Chlorophyll; C032623:pheophorbide a