title: A novel C1q-domain-containing (C1qDC) protein from Mytilus coruscus with the transcriptional analysis against marine pathogens and heavy metals.
abstract: The C1q-domain-containing (C1qDC) proteins, which are involved in various processes of vertebrates, are important pattern recognition receptors in innate immunity of invertebrates. In present study, a novel C1qDC was identified from Mytilus coruscus (designated as McC1qDC), which was 917 bp in length encoding 236 amino acids with a typical signal peptide of 19 amino acid residues in N-terminus. Based on its conserved C1q domain and molecular architecture of 10 β-strand jelly-roll folding topology structure, McC1qDC might be classified as a member of the C1q family. The mRNA transcript of McC1qDC was predominantly detectable in the hemocytes, and a less degree in gill, gonad and mantle, but trace in foot, adductor and digestive gland. Upon induction by Vibrio harveyi and Vibrio alginolyticus, McC1qDC expression was significantly up-regulated. Time-dependent mRNA expression of McC1qDC was found during copper and cadmium exposure for its heavy metal-binding domain. These results indicated that McC1qDC was a novel member of the C1qDC protein family as a pattern recognition receptor against pathogens, and might be developed as a potential indicator for monitoring heavy metals pollution.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310012, People's Republic of China; School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316004, People's Republic of China.
authors.forename: Hui-Hui
authors.initials: HH
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310012, People's Republic of China.
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Xiang
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310012, People's Republic of China. Electronic address: shaojz@zju.edu.cn.
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Shao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002104:Cadmium / Q000009:adverse effects; D015922:Complement C1q / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003300:Copper / Q000009:adverse effects; D004781:Environmental Exposure / Q000009:adverse effects; D005880:Gills / Q000276:immunology*; D006434:Hemocytes / Q000276:immunology*; D007113:Immunity, Innate; D008969:Molecular Sequence Data; D049878:Mytilus / Q000276:immunology*; D010802:Phylogeny; D012333:RNA, Messenger / Q000032:analysis; D051192:Receptors, Pattern Recognition / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015854:Up-Regulation; D014733:Vibrio / Q000276:immunology*; D014735:Vibrio Infections / Q000276:immunology*
chemical_list: D012333:RNA, Messenger; D051192:Receptors, Pattern Recognition; D002104:Cadmium; D003300:Copper; D015922:Complement C1q
keywords: Biomarker; C1q-domain-containing protein (C1qDC); Innate immunity; Mytilus coruscus; Pattern recognition receptor