title: The role of semidisorder in temperature adaptation of bacterial FlgM proteins.
abstract: Probabilities of disorder for FlgM proteins of 39 species whose optimal growth temperature ranges from 273 K (0°C) to 368 K (95°C) were predicted by a newly developed method called Sequence-based Prediction with Integrated NEural networks for Disorder (SPINE-D). We showed that the temperature-dependent behavior of FlgM proteins could be separated into two subgroups according to their sequence lengths. Only shorter sequences evolved to adapt to high temperatures (>318 K or 45°C). Their ability to adapt to high temperatures was achieved through a transition from a fully disordered state with little secondary structure to a semidisordered state with high predicted helical probability at the N-terminal region. The predicted results are consistent with available experimental data. An analysis of all orthologous protein families in 39 species suggests that such a transition from a fully disordered state to semidisordered and/or ordered states is one of the strategies employed by nature for adaptation to high temperatures.
authors: Shandong Provincial Key Laboratory of Functional Macromolecular Biophysics, Dezhou University, Dezhou, Shandong Province China; School of Physics and Electronic Information, Dezhou University, Dezhou, Shandong Province China.
authors: Jihua
authors: J
authors: Wang
authors: Yuedong
authors: Y
authors: Yang
authors: Zanxia
authors: Z
authors: Cao
authors: Zhixiu
authors: Z
authors: Li
authors: Huiying
authors: H
authors: Zhao
authors: Yaoqi
authors: Y
authors: Zhou
mesh_terms: D000222:Adaptation, Physiological; D000465:Algorithms; D000595:Amino Acid Sequence; D001419:Bacteria / Q000737:chemistry / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D006358:Hot Temperature; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D011487:Protein Conformation; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; C071266:FlgM protein, Bacteria