title: Improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration.
abstract: The use of controlled dehydration for improvement of protein crystal diffraction quality is increasing in popularity, although there are still relatively few documented examples of success. A study has been carried out to establish whether controlled dehydration could be used to improve the anisotropy of crystals of the core protein of the human proteoglycan glypican-1. Crystals were subjected to controlled dehydration using the HC1 device. The optimal protocol for dehydration was developed by careful investigation of the following parameters: dehydration rate, final relative humidity and total incubation time Tinc. Of these, the most important was shown to be Tinc. After dehydration using the optimal protocol the crystals showed significantly reduced anisotropy and improved electron density, allowing the building of previously disordered parts of the structure.
authors: Department of Biochemistry and Structural Biology, Centre for Molecular Protein Science, Lund University, Box 124, 221 00 Lund, Sweden.
authors: Wael
authors: W
authors: Awad
authors: Gabriel
authors: G
authors: Svensson Birkedal
authors: Marjolein M G M
authors: MM
authors: Thunnissen
authors: Katrin
authors: K
authors: Mani
authors: Derek T
authors: DT
authors: Logan
mesh_terms: D016880:Anisotropy; D003460:Crystallization / Q000379:methods*; D018360:Crystallography, X-Ray / Q000379:methods; D004583:Electrons; D053673:Glypicans / Q000737:chemistry*; D006801:Humans; D008958:Models, Molecular; D011487:Protein Conformation; D014867:Water / Q000737:chemistry*
chemical_list: D053673:Glypicans; D014867:Water
keywords: HC1; crystal dehydration; crystal packing; diffraction anisotropy; glypican-1; optimization