title: Changes in sugar content and proteome of potato in response to cold and dehydration stress and their implications for cryopreservation.
abstract: The key to successful cryopreservation lies in the induction of tolerance towards dehydration/desiccation and freezing. The accumulation of osmo-active compounds, which can be induced by drought and cold stress, is therefore important. In the present study, three-week old shoots from in vitro plantlets of the cultivated potato Solanum tuberosum and its frost-resistant relative Solanum commersonii were submitted to osmotic stress (by using sucrose) and chilling (6°C). After 14days of exposure, shoot tips were sampled in order to gain an insight into changes of the proteome and soluble sugars. Also, the effect of these treatments on growth performance behaviour and on the success of cryopreservation was evaluated. Identified proteins that changed in abundance due to stress were associated with stress response. Additionally, carbohydrate analyses in both species, after exposure to chilling, also indicated species-related differences; this observation could point towards a better-adapted physiological state of the donor plants of S. commersonii prior to the cryoprocedure and therefore a better recovery of the meristems.
  
  
  CONCLUSIONS
  To our knowledge, this is the first study in which cryopreservation experiments are combined with the observation of the responses to abiotic stress exposure involving the potato species S. commersonii and S. tuberosum. These two species are known to have a different cold-acclimation behaviour, which seems to be closely related to their tolerance towards cryopreservation. Furthermore, common and differential responses to abiotic stresses were observed in the two species indicating that some pathways could be crucial not only in the plant's response to stress but also in tolerance towards cryopreservation.
authors: Environment and Agro-biotechnologies department (EVA), Centre de Recherche Public-Gabriel Lippmann, 41, rue du Brill, L-4422 Belvaux, Luxembourg; Laboratory of Tropical Crop Improvement, Division of Crop Biotechnics, KU Leuven, Willem de Croylaan 42 bus 2455, B-3001 Leuven, Belgium.
authors: Raquel
authors: R
authors: Folgado
authors: Environment and Agro-biotechnologies department (EVA), Centre de Recherche Public-Gabriel Lippmann, 41, rue du Brill, L-4422 Belvaux, Luxembourg.
authors: Kjell
authors: K
authors: Sergeant
authors: Environment and Agro-biotechnologies department (EVA), Centre de Recherche Public-Gabriel Lippmann, 41, rue du Brill, L-4422 Belvaux, Luxembourg.
authors: Jenny
authors: J
authors: Renaut
authors: Laboratory of Tropical Crop Improvement, Division of Crop Biotechnics, KU Leuven, Willem de Croylaan 42 bus 2455, B-3001 Leuven, Belgium; Bioversity International, Willem de Croylaan 42 bus 2455, B-3001 Leuven, Belgium; International Institute of Tropical Agriculture, POB 10, Duluti, Arusha, Tanzania.
authors: Rony
authors: R
authors: Swennen
authors: Environment and Agro-biotechnologies department (EVA), Centre de Recherche Public-Gabriel Lippmann, 41, rue du Brill, L-4422 Belvaux, Luxembourg.
authors: Jean-Francois
authors: JF
authors: Hausman
authors: Bioversity International, Willem de Croylaan 42 bus 2455, B-3001 Leuven, Belgium.
authors: Bart
authors: B
authors: Panis
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002241:Carbohydrates; D015925:Cryopreservation; D009997:Osmotic Pressure; D020543:Proteome / Q000378:metabolism*; D011198:Solanum tuberosum / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D001426:Bacterial Proteins; D002241:Carbohydrates; D020543:Proteome
keywords: Abiotic stress; Cold; Cryopreservation; Heat shock proteins; Potato; Soluble sugars