title: Effects of aging, cytomegalovirus infection, and EBV infection on human B cell repertoires.
abstract: Elderly humans show decreased humoral immunity to pathogens and vaccines, yet the effects of aging on B cells are not fully known. Chronic viral infection by CMV is implicated as a driver of clonal T cell proliferations in some aging humans, but whether CMV or EBV infection contributes to alterations in the B cell repertoire with age is unclear. We have used high-throughput DNA sequencing of IGH gene rearrangements to study the BCR repertoires over two successive years in 27 individuals ranging in age from 20 to 89 y. Some features of the B cell repertoire remain stable with age, but elderly subjects show increased numbers of B cells with long CDR3 regions, a trend toward accumulation of more highly mutated IgM and IgG Ig genes, and persistent clonal B cell populations in the blood. Seropositivity for CMV or EBV infection alters B cell repertoires, regardless of the individual's age: EBV infection correlates with the presence of persistent clonal B cell expansions, whereas CMV infection correlates with the proportion of highly mutated Ab genes. These findings isolate effects of aging from those of chronic viral infection on B cell repertoires and provide a baseline for understanding human B cell responses to vaccination or infectious stimuli.
authors: Department of Pathology, Stanford University, Stanford, CA 94305;
authors: Chen
authors: C
authors: Wang
authors: Yi
authors: Y
authors: Liu
authors: Lan T
authors: LT
authors: Xu
authors: Katherine J L
authors: KJ
authors: Jackson
authors: Krishna M
authors: KM
authors: Roskin
authors: Tho D
authors: TD
authors: Pham
authors: Jonathan
authors: J
authors: Laserson
authors: Eleanor L
authors: EL
authors: Marshall
authors: Katie
authors: K
authors: Seo
authors: Ji-Yeun
authors: JY
authors: Lee
authors: David
authors: D
authors: Furman
authors: Daphne
authors: D
authors: Koller
authors: Cornelia L
authors: CL
authors: Dekker
authors: Mark M
authors: MM
authors: Davis
authors: Andrew Z
authors: AZ
authors: Fire
authors: Scott D
authors: SD
authors: Boyd
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000276:immunology*; D000914:Antibodies, Viral / Q000235:genetics / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology* / Q000821:virology; D020031:Epstein-Barr Virus Infections / Q000276:immunology* / Q000821:virology; D005803:Genes, Immunoglobulin / Q000235:genetics / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008875:Middle Aged; D009154:Mutation / Q000235:genetics / Q000276:immunology; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral