title: In vitro and in vivo effects of sub-MICs of pexiganan and imipenem on Pseudomonas aeruginosa adhesion and biofilm development.
abstract: An in vitro and in vivo study was performed to quantify adhesion and biofilm formation ability of Pseudomonas aeruginosa slime producer under the effect of sub-minimal inhibitory concentrations (MICs) of pexiganan and imipenem. To evaluate adherence, squares of ureteral stents were placed in six-well tissue-culture plates containing 6 ml of a cell suspension grown in the presence of sub-MICs of study antibiotics. To evaluate biofilm formation sterilized squares were placed in six-well tissue culture plates containing 6 ml of triptic soy broth (TSB) supplemented with 0.25% of glucose and the respective amount of antibiotic. For in vivo study a biofilm infection rat model was performed. The study included an uninfected control group to evaluate the sterility of surgical procedure, a group infected with a slime-producer P. aeruginosa strain not previously treated with antibiotics and two groups infected with the strain previously treated with imipenem or pexiganan. Adherence and biofilm in vitro formation was strongly affected by pre-treatment with pexiganan and imipenem, with the latter being the more effective antibiotic. The in vivo results showed a reduction in bacterial load on the ureteral stent tissue of the pre-treated strain. Differently, urine cultures showed no differences in bacterial growth for the pre-treated strain showing that it retained its ability to cause infection. This study suggests that sub-MIC imipenem and pexiganan could be a good strategy to target the adhesion process during the infection cycle.
authors: Clinic of Infectious Diseases, Universita Politecnica delle Marche, Ospedali Riuniti; General Surgery and Surgery Methodology Clinic, Universita Politecnica delle Marche, Ospedali Riuniti, Ancona, Italy; Faculty of Chemistry, University of Gdansk, Gdansk, Poland; Institute of Maternal and Childrens Sciences-Urology, Polytechnic University of the Marche Region, A.O. Ospedali Riuniti, Ancona, Italy; Experimental Animal Models for Aging Units, Research Department, I.N.R.C.A. I.R.R.C.S., Ancona, Italy; Faculty of Pharmacy, Medical University of Gdansk, Gdansk, Poland.
authors: Oscar
authors: O
authors: Cirioni
authors: Clinic of Infectious Diseases, Universita Politecnica delle Marche, Ospedali Riuniti; General Surgery and Surgery Methodology Clinic, Universita Politecnica delle Marche, Ospedali Riuniti, Ancona, Italy; Faculty of Chemistry, University of Gdansk, Gdansk, Poland; Institute of Maternal and Childrens Sciences-Urology, Polytechnic University of the Marche Region, A.O. Ospedali Riuniti, Ancona, Italy; Experimental Animal Models for Aging Units, Research Department, I.N.R.C.A. I.R.R.C.S., Ancona, Italy; Faculty of Pharmacy, Medical University of Gdansk, Gdansk, Poland.
authors: Carmela
authors: C
authors: Silvestri
authors: Clinic of Infectious Diseases, Universita Politecnica delle Marche, Ospedali Riuniti; General Surgery and Surgery Methodology Clinic, Universita Politecnica delle Marche, Ospedali Riuniti, Ancona, Italy; Faculty of Chemistry, University of Gdansk, Gdansk, Poland; Institute of Maternal and Childrens Sciences-Urology, Polytechnic University of the Marche Region, A.O. Ospedali Riuniti, Ancona, Italy; Experimental Animal Models for Aging Units, Research Department, I.N.R.C.A. I.R.R.C.S., Ancona, Italy; Faculty of Pharmacy, Medical University of Gdansk, Gdansk, Poland.
authors: Roberto
authors: R
authors: Ghiselli
authors: Clinic of Infectious Diseases, Universita Politecnica delle Marche, Ospedali Riuniti; General Surgery and Surgery Methodology Clinic, Universita Politecnica delle Marche, Ospedali Riuniti, Ancona, Italy; Faculty of Chemistry, University of Gdansk, Gdansk, Poland; Institute of Maternal and Childrens Sciences-Urology, Polytechnic University of the Marche Region, A.O. Ospedali Riuniti, Ancona, Italy; Experimental Animal Models for Aging Units, Research Department, I.N.R.C.A. I.R.R.C.S., Ancona, Italy; Faculty of Pharmacy, Medical University of Gdansk, Gdansk, Poland.
authors: Wojciech
authors: W
authors: Kamysz
authors: Clinic of Infectious Diseases, Universita Politecnica delle Marche, Ospedali Riuniti; General Surgery and Surgery Methodology Clinic, Universita Politecnica delle Marche, Ospedali Riuniti, Ancona, Italy; Faculty of Chemistry, University of Gdansk, Gdansk, Poland; Institute of Maternal and Childrens Sciences-Urology, Polytechnic University of the Marche Region, A.O. Ospedali Riuniti, Ancona, Italy; Experimental Animal Models for Aging Units, Research Department, I.N.R.C.A. I.R.R.C.S., Ancona, Italy; Faculty of Pharmacy, Medical University of Gdansk, Gdansk, Poland.
authors: Daniele
authors: D
authors: Minardi
authors: Clinic of Infectious Diseases, Universita Politecnica delle Marche, Ospedali Riuniti; General Surgery and Surgery Methodology Clinic, Universita Politecnica delle Marche, Ospedali Riuniti, Ancona, Italy; Faculty of Chemistry, University of Gdansk, Gdansk, Poland; Institute of Maternal and Childrens Sciences-Urology, Polytechnic University of the Marche Region, A.O. Ospedali Riuniti, Ancona, Italy; Experimental Animal Models for Aging Units, Research Department, I.N.R.C.A. I.R.R.C.S., Ancona, Italy; Faculty of Pharmacy, Medical University of Gdansk, Gdansk, Poland.
authors: Pamela
authors: P
authors: Castelli
authors: Clinic of Infectious Diseases, Universita Politecnica delle Marche, Ospedali Riuniti; General Surgery and Surgery Methodology Clinic, Universita Politecnica delle Marche, Ospedali Riuniti, Ancona, Italy; Faculty of Chemistry, University of Gdansk, Gdansk, Poland; Institute of Maternal and Childrens Sciences-Urology, Polytechnic University of the Marche Region, A.O. Ospedali Riuniti, Ancona, Italy; Experimental Animal Models for Aging Units, Research Department, I.N.R.C.A. I.R.R.C.S., Ancona, Italy; Faculty of Pharmacy, Medical University of Gdansk, Gdansk, Poland.
authors: Fiorenza
authors: F
authors: Orlando
authors: Clinic of Infectious Diseases, Universita Politecnica delle Marche, Ospedali Riuniti; General Surgery and Surgery Methodology Clinic, Universita Politecnica delle Marche, Ospedali Riuniti, Ancona, Italy; Faculty of Chemistry, University of Gdansk, Gdansk, Poland; Institute of Maternal and Childrens Sciences-Urology, Polytechnic University of the Marche Region, A.O. Ospedali Riuniti, Ancona, Italy; Experimental Animal Models for Aging Units, Research Department, I.N.R.C.A. I.R.R.C.S., Ancona, Italy; Faculty of Pharmacy, Medical University of Gdansk, Gdansk, Poland.
authors: Elzbieta
authors: E
authors: Kamysz
authors: Clinic of Infectious Diseases, Universita Politecnica delle Marche, Ospedali Riuniti; General Surgery and Surgery Methodology Clinic, Universita Politecnica delle Marche, Ospedali Riuniti, Ancona, Italy; Faculty of Chemistry, University of Gdansk, Gdansk, Poland; Institute of Maternal and Childrens Sciences-Urology, Polytechnic University of the Marche Region, A.O. Ospedali Riuniti, Ancona, Italy; Experimental Animal Models for Aging Units, Research Department, I.N.R.C.A. I.R.R.C.S., Ancona, Italy; Faculty of Pharmacy, Medical University of Gdansk, Gdansk, Poland.
authors: Mauro
authors: M
authors: Provinciali
authors: Clinic of Infectious Diseases, Universita Politecnica delle Marche, Ospedali Riuniti; General Surgery and Surgery Methodology Clinic, Universita Politecnica delle Marche, Ospedali Riuniti, Ancona, Italy; Faculty of Chemistry, University of Gdansk, Gdansk, Poland; Institute of Maternal and Childrens Sciences-Urology, Polytechnic University of the Marche Region, A.O. Ospedali Riuniti, Ancona, Italy; Experimental Animal Models for Aging Units, Research Department, I.N.R.C.A. I.R.R.C.S., Ancona, Italy; Faculty of Pharmacy, Medical University of Gdansk, Gdansk, Poland.
authors: Giovanni
authors: G
authors: Muzzonigro
authors: Clinic of Infectious Diseases, Universita Politecnica delle Marche, Ospedali Riuniti; General Surgery and Surgery Methodology Clinic, Universita Politecnica delle Marche, Ospedali Riuniti, Ancona, Italy; Faculty of Chemistry, University of Gdansk, Gdansk, Poland; Institute of Maternal and Childrens Sciences-Urology, Polytechnic University of the Marche Region, A.O. Ospedali Riuniti, Ancona, Italy; Experimental Animal Models for Aging Units, Research Department, I.N.R.C.A. I.R.R.C.S., Ancona, Italy; Faculty of Pharmacy, Medical University of Gdansk, Gdansk, Poland.
authors: Mario
authors: M
authors: Guerrieri
authors: Clinic of Infectious Diseases, Universita Politecnica delle Marche, Ospedali Riuniti; General Surgery and Surgery Methodology Clinic, Universita Politecnica delle Marche, Ospedali Riuniti, Ancona, Italy; Faculty of Chemistry, University of Gdansk, Gdansk, Poland; Institute of Maternal and Childrens Sciences-Urology, Polytechnic University of the Marche Region, A.O. Ospedali Riuniti, Ancona, Italy; Experimental Animal Models for Aging Units, Research Department, I.N.R.C.A. I.R.R.C.S., Ancona, Italy; Faculty of Pharmacy, Medical University of Gdansk, Gdansk, Poland.
authors: Andrea
authors: A
authors: Giacometti
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D023181:Antimicrobial Cationic Peptides / Q000008:administration & dosage*; D001422:Bacterial Adhesion / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects*; D005260:Female; D006801:Humans; D015378:Imipenem / Q000008:administration & dosage*; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D015607:Stents / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D015378:Imipenem; C093828:pexiganan