title: Assessing the detection capacity of microarrays as bio/nanosensing platforms.
abstract: Microarray is one of the most powerful detection systems with multiplexing and high throughput capability. It has significant potential as a versatile biosensing platform for environmental monitoring, pathogen detection, medical therapeutics, and drug screening to name a few. To date, however, microarray applications are still limited to preliminary screening of genome-scale transcription profiling or gene ontology analysis. Expanding the utility of microarrays as a detection tool for various biological and biomedical applications requires information about performance such as the limits of detection and quantification, which are considered as an essential information to decide the detection sensitivity of sensing devices. Here we present a calibration design that integrates detection limit theory and linear dynamic range to obtain a performance index of microarray detection platform using oligonucleotide arrays as a model system. Two different types of limits of detection and quantification are proposed by the prediction or tolerance interval for two common cyanine fluorescence dyes, Cy3 and Cy5. Besides oligonucleotide, the proposed method can be generalized to other microarray formats with various biomolecules such as complementary DNA, protein, peptide, carbohydrate, tissue, or other small biomolecules. Also, it can be easily applied to other fluorescence dyes for further dye chemistry improvement.
authors: Bio/Nano Technology Laboratory, Institute for Nanoscience and Engineering, University of Arkansas, Fayetteville, AR 72701, USA ; Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA ; Graduate Program in Cell and Molecular Biology, University of Arkansas, Fayetteville, AR 72701, USA ; Department of Chemistry, Seoul National University, Seoul 151-747, Republic of Korea.
authors: Ju Seok
authors: JS
authors: Lee
authors: Joon Jin
authors: JJ
authors: Song
authors: Russell
authors: R
authors: Deaton
authors: Jin-Woo
authors: JW
authors: Kim
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D046228:Microarray Analysis / Q000379:methods*; D008962:Models, Theoretical