title: Sphingolipid levels crucially modulate lateral microdomain organization of plasma membrane in living yeast.
abstract: We report sphingolipid-related reorganization of gel-like microdomains in the plasma membrane of living Saccharomyces cerevisiae using trans-Parinaric acid (t-PnA) and 1,6-diphenyl-1,3,5-hexatriene (DPH). Compared to control, the gel-like domains were significantly reduced in the membrane of a sphingolipid-deficient lcb1-100 mutant. The same reduction resulted from sphingolipid depletion by myriocin. The phenotype could be reverted when a myriocin-induced block in sphingolipid biosynthesis was bypassed by exogenous dihydrosphingosine. Lipid order of less-ordered membrane regions decreased with sphingolipid depletion as well, as documented by DPH fluorescence anisotropy. The data indicate that organization of lateral microdomains is an essential physiological role of these structural lipids.
authors: Faculty of Mathematics and Physics, Charles University, Ke Karlovu 5, 121 16 Prague 2, Czech Republic.
authors: Jaroslav
authors: J
authors: Vecer
authors: Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic.
authors: Petra
authors: P
authors: Vesela
authors: Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic. Electronic address: malinsky@biomed.cas.cz.
authors: Jan
authors: J
authors: Malinsky
authors: Faculty of Mathematics and Physics, Charles University, Ke Karlovu 5, 121 16 Prague 2, Czech Republic. Electronic address: herman@karlov.mff.cuni.cz.
authors: Petr
authors: P
authors: Herman
mesh_terms: D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D004161:Diphenylhexatriene / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D005454:Fluorescence Polarization; D021962:Membrane Microdomains / Q000737:chemistry / Q000378:metabolism*; D009154:Mutation; D012441:Saccharomyces cerevisiae; D013050:Spectrometry, Fluorescence; D013107:Sphingolipids / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D005231:Fatty Acids, Unsaturated; D013107:Sphingolipids; D004161:Diphenylhexatriene; C013641:parinaric acid
keywords: 1,6-diphenyl-1,3,5-hexatriene; DHS; DL-dihydrosphingosine; DPH; Fluidity; Fluorescence anisotropy; Lipid order; Membrane microdomain; Time-resolved fluorescence; all-trans-9,11,13,15-octadecatetraenoic acid; t-PnA