title: Elevated expression of Piwi and piRNAs in ovaries of triploid crucian carp.
abstract: Increasing piRNAs provide RNA-interference pathways to regulate transposons and gene expression in germline cells. We demonstrate that Piwi transcripts are exclusively expressed in adult testes and ovaries in teleosts, with triploids showing the highest Piwi expression in the ovaries. Studies in vivo and in vitro showed that hCG and E2 treatment suppressed Piwi expression. We further cloned 200 small RNAs in the three kinds of fish. Seven piRNAs were obtained from all the three different ploidy fishes. During ovulation, five piRNAs showed significantly higher expression in the ovaries of sterile triploids than fertile diploids and tetraploids. Furthermore, E2 suppressed the expression of the six piRNAs at different levels in vivo and in vitro. The present study bridges the gap between the HPG axis and Piwi-piRNA pathway by suggesting that a dysfunctional HPG axis abrogated the piRNA suppression in triploid fish.
authors: Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
authors: Yi
authors: Y
authors: Zhou
authors: Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
authors: Huan
authors: H
authors: Zhong
authors: Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China. Electronic address: lsj@hunnu.edu.cn.
authors: Shaojun
authors: S
authors: Liu
authors: Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
authors: Fan
authors: F
authors: Yu
authors: Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
authors: Jie
authors: J
authors: Hu
authors: Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
authors: Chun
authors: C
authors: Zhang
authors: Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
authors: Min
authors: M
authors: Tao
authors: Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
authors: Yun
authors: Y
authors: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics*; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D015232:Dinoprostone / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D011003:Ploidies; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism; D012098:Reproduction / Q000235:genetics*; D016415:Sequence Alignment; D015398:Signal Transduction
chemical_list: D006063:Chorionic Gonadotropin; D034741:RNA, Small Interfering; D015232:Dinoprostone
keywords: HPG axis; Piwi; Tetraploid; Triploid; piRNAs