title: Hantavirus Gn and Gc glycoproteins self-assemble into virus-like particles.
abstract: How hantaviruses assemble and exit infected cells remains largely unknown. Here, we show that the expression of Andes (ANDV) and Puumala (PUUV) hantavirus Gn and Gc envelope glycoproteins lead to their self-assembly into virus-like particles (VLPs) which were released to cell supernatants. The viral nucleoprotein was not required for particle formation. Further, a Gc endodomain deletion mutant did not abrogate VLP formation. The VLPs were pleomorphic, exposed protrusions and reacted with patient sera.
authors: Molecular Virology Laboratory, Fundación Ciencia & Vida, Santiago, Chile.
authors: Rodrigo
authors: R
authors: Acuña
authors: Nicolás
authors: N
authors: Cifuentes-Muñoz
authors: Chantal L
authors: CL
authors: Márquez
authors: Manuela
authors: M
authors: Bulling
authors: Jonas
authors: J
authors: Klingström
authors: Roberta
authors: R
authors: Mancini
authors: Pierre-Yves
authors: PY
authors: Lozach
authors: Nicole D
authors: ND
authors: Tischler
mesh_terms: D015153:Blotting, Western; D003429:Cross Reactions / Q000276:immunology; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D006476:Orthohantavirus / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D029262:Puumala virus / Q000235:genetics / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014771:Virion / Q000235:genetics / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D014759:Viral Envelope Proteins