title: Absence of primary cilia in cell cycle-arrested human breast cancer cells.
abstract: Previous studies using cultured cells showed that primary cilia are present in quiescent cells, but are absent in proliferating cells. We studied here the relationship between the presence or absence of primary cilia and the cell cycle arrest of normal epithelial cells and cancer cells in the human normal breast and breast cancer tissues. In normal breast tissues, although most epithelial cells were nonproliferating as estimated by the immunofluorescence staining of the proliferation marker Ki-67, primary cilia were present only in 20-40% of the epithelial cells. In breast cancer tissues, primary cilia were not observed in any of the breast cancer cells. Furthermore, primary cilia were hardly observed in the nonproliferating cancer cells in the orthotopic and metastatic human breast cancer xenograft tumors in mice. These results indicate that the absence of primary cilia does not necessarily represent the proliferating phases of normal epithelial cells and cancer cells.
authors: Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 1-5-6 Minatojima-minamimachi, Chuo-ku, Kobe, 650-0047, Japan; Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan; Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan.
authors: Kentaro
authors: K
authors: Nobutani
authors: Yohei
authors: Y
authors: Shimono
authors: Midori
authors: M
authors: Yoshida
authors: Kiyohito
authors: K
authors: Mizutani
authors: Akihiro
authors: A
authors: Minami
authors: Seishi
authors: S
authors: Kono
authors: Toru
authors: T
authors: Mukohara
authors: Takashi
authors: T
authors: Yamasaki
authors: Tomoo
authors: T
authors: Itoh
authors: Shintaro
authors: S
authors: Takao
authors: Hironobu
authors: H
authors: Minami
authors: Takeshi
authors: T
authors: Azuma
authors: Yoshimi
authors: Y
authors: Takai
mesh_terms: D000818:Animals; D001940:Breast / Q000473:pathology; D001943:Breast Neoplasms / Q000473:pathology*; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D002923:Cilia / Q000473:pathology; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D064593:Heterografts; D006801:Humans; D051379:Mice; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation