title: Simultaneous electrochemical detection of cervical cancer markers using reduced graphene oxide-tetraethylene pentamine as electrode materials and distinguishable redox probes as labels.
abstract: A novel, highly sensitive electrochemical immunoassay was proposed for the simultaneous determination of carcinoembryonic antigen (CEA) and squamous cell carcinoma antigen (SCCA) for the diagnosis of cervical cancer. Using an electrochemical analysis technique, two well-separated peaks were generated by neutral red and thionine, making the simultaneous detection of the two analytes on the electrode possible. Reduced graphene oxide-tetraethylene pentamine (rGO-TEPA), containing more amino groups, was of benefit to immobilize the primary antibody (Ab1) through an amidation reaction. Au@mesoporous carbon CMK-3 was synthesized and incubated with two secondary antibodies (Ab2) and different redox probes (neutral red and thionine) to fabricate the electrochemical immunosensor label intending to improve the analytical performance of the immunosensor. The immunosensor was prepared with a sandwich structure based on the peak current change of neutral red and thionine before and after the antigen-antibody reaction. The results showed that the immunosensor had a wide linear range, low detection limit, good reproducibility and stability. The method has been applied to the analysis of serum samples with satisfactory results.
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Dan
authors: D
authors: Wu
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Aiping
authors: A
authors: Guo
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Zhankui
authors: Z
authors: Guo
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Lili
authors: L
authors: Xie
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China. Electronic address: sdjndxwq@163.com.
authors: Qin
authors: Q
authors: Wei
authors: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors: Bin
authors: B
authors: Du
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis / Q000097:blood*; D014408:Biomarkers, Tumor / Q000032:analysis / Q000097:blood*; D015374:Biosensing Techniques / Q000295:instrumentation*; D002272:Carcinoembryonic Antigen / Q000032:analysis / Q000097:blood*; D055664:Electrochemical Techniques / Q000295:instrumentation; D004566:Electrodes; D005260:Female; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation; D057230:Limit of Detection; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D015203:Reproducibility of Results; D015843:Serpins / Q000032:analysis / Q000097:blood*; D002583:Uterine Cervical Neoplasms / Q000097:blood* / Q000175:diagnosis
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D010087:Oxides; D000644:Quaternary Ammonium Compounds; D015843:Serpins; C051072:squamous cell carcinoma-related antigen; C040648:pentamine; D006108:Graphite
keywords: Au@mesoporous carbon CMK-3; Redox probe; Reduced graphene oxide-tetraethylene pentamine (rGO-TEPA); Simultaneous electrochemical detection; Tumor markers