title: Cloning and characterization of a novel dehydrin gene, SiDhn2, from Saussurea involucrata Kar. et Kir.
abstract: Saussurea involucrata Kar. et Kir. is a hardy dicotyledonous plant capable of tolerating severe abiotic stress conditions. In a previous study, we created a cDNA library to determine what factors are associated with the cold acclimation response in S. involucrata. From this, a full-length cDNA of a dehydrin-like gene (SiDhn2) was obtained by RT-PCR. The SiDhn2 gene was characterized in this study. The full-length SiDhn2 cDNA comprised 693 bp containing an open reading frame of 345 bp specifying a protein of 115 amino acids. An alignment of the deduced amino acid sequence showed that SiDhn2 shared 55 % identity with two Brassica dehydrins. Agrobacterium tumefaciens was used to transform RD29A:SiDhn2 and 35S:SiDhn2 constructs into tobacco to investigate the germination and resistance to freezing and drought stress of transgenic plants. The RD29A:SiDhn2 transgenic plants showed greater resistance to freezing and drought stress than 35S:SiDhn2 transgenic plants or the wild-type. This study demonstrates that SiDhn2 confers cold hardiness and drought resistance, and may be a candidate resistance gene for genetic improvement of crops to increase stress resistance.
authors: Laboratory of Agricultural Biotechnology, College of Life Science of Shihezi University, Shihezi, 832003, Xinjiang, China.
authors: Honglin
authors: H
authors: Qiu
authors: Linhua
authors: L
authors: Zhang
authors: Chao
authors: C
authors: Liu
authors: Li
authors: L
authors: He
authors: Aiying
authors: A
authors: Wang
authors: Hai-Liang
authors: HL
authors: Liu
authors: Jian-Bo
authors: JB
authors: Zhu
mesh_terms: D000064:Acclimatization; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D055864:Droughts; D005615:Freezing; D005786:Gene Expression Regulation; D015723:Gene Library; D018525:Germination; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D030441:Saussurea / Q000235:genetics* / Q000502:physiology; D016415:Sequence Alignment; D013312:Stress, Physiological; D014026:Nicotiana / Q000235:genetics / Q000502:physiology
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; C070556:dehydrin proteins, plant