title: Preparation and characterization of galactosylated glycol chitosan micelles and its potential use for hepatoma-targeting delivery of doxorubicin.
abstract: This study aimed to develop novel galactosylated cholesterol modified-glycol chitosan (Gal-CHGC) micelles for targeting delivery of doxorubicin (DOX) in live cancer cells. Three kinds of Gal-CHGC conjugates were synthesized and characterized. The mean particle size and critical aggregation concentration of these polymeric micelles increased with the increase of galactose substitution degree. The DOX-loaded micelles were prepared by an o/w method. The mean diameters of DOX-loaded galactosylated micelles were in the range of 387-497 nm. DOX released from drug-loaded micelles displayed a biphasic way. Cellular uptake studies demonstrated that DOX-loaded galactosylated micelles could enhance the uptake of DOX into HepG2 cells. Moreover, the cytotoxicity of DOX-loaded galactosylated micelles against HepG2 cells significantly improved in contrast with free DOX and DOX-loaded micelles without galactosylation. These results suggested that Gal-CHGC micelles could be a potential carrier for hepatoma-targeting drug delivery.
authors: Department of Pharmacy, College of Basic Medical Science, Jiujiang University, 17 Lufeng Road, Jiujiang, 332000, People's Republic of China, yjm1016@hotmail.com.
authors: Jing-Mou
authors: JM
authors: Yu
authors: Wei-Dong
authors: WD
authors: Li
authors: Lu
authors: L
authors: Lu
authors: Xue-Yun
authors: XY
authors: Zhou
authors: Dian-Yuan
authors: DY
authors: Wang
authors: Hui-Min
authors: HM
authors: Li
authors: Xiao-Yuan
authors: XY
authors: Xu
authors: Jian
authors: J
authors: Chen
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects*; D048271:Chitosan / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000138:chemical synthesis*; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics*; D005240:Feasibility Studies; D005690:Galactose / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D008422:Materials Testing; D008823:Micelles; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size
chemical_list: D000903:Antibiotics, Antineoplastic; D003692:Delayed-Action Preparations; D008823:Micelles; D053769:Nanocapsules; C121252:glycated chitosan; D004317:Doxorubicin; D048271:Chitosan; D005690:Galactose