title: Stabilization of dentin matrix after cross-linking treatments, in vitro.
abstract: OBJECTIVE
  To evaluate the effect of EDC on elastic modulus (E), MMPs activity, hydroxyproline (HYP) release and thermal denaturation temperature of demineralized dentin collagen.
  
  
  METHODS
  Dentin beams were obtained from human molars and completely demineralized in 10 wt% H3PO4 for 18 h. The initial E and MMP activity were determined with three-point bending and microcolorimetric assay, respectively. Extra demineralized beams were dehydrated and the initial dry mass (DM) was determined. All the beams were distributed into groups (n=10) and treated for 30 s or 60 s with: water, 0.5 M, 1 M or 2 M EDC or 10% glutaraldehyde (GA). After treatment, the new E and MMP activity were redetermined. The beams submitted to DM measurements were storage for 1 week in artificial saliva, after that the mass loss and HYP release were evaluated. The collagen thermal denaturation temperature (TDT) was determined by DSC analysis. Data for E, MMP activity and HYP release were submitted to Wilcoxon and Kruskal-Wallis or Mann-Whitney tests. Mass loss and TDT data were submitted to ANOVA and Tukey tests at the 5% of significance.
  
  
  RESULTS
  EDC was able to significantly increase collagen stiffness in 60s. 10% GA groups obtained the highest E values after both 30 and 60s. All cross-linking agents decreased MMP activity and HYP release and increased TDT temperature. Significant differences were identified among EDC groups after 30 or 60 s of cross-linking, 1M or 2M EDC showed the lowest MMP activity.
  
  
  CONCLUSIONS
  Cross-linking agents are capable of preventing dentin collagen degradation. EDC treatment may be clinically useful to increase resin-dentin stability.
authors: Department of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, UNESP - Univ Estadual Paulista, Araraquara, São Paulo, Brazil.
authors: Débora L S
authors: DL
authors: Scheffel
authors: Department of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, UNESP - Univ Estadual Paulista, Araraquara, São Paulo, Brazil.
authors: Josimeri
authors: J
authors: Hebling
authors: Department of Oral Biology, College of Dental Medicine, Georgia Regents University, Augusta, GA, USA.
authors: Régis H
authors: RH
authors: Scheffel
authors: Department of Oral Biology, College of Dental Medicine, Georgia Regents University, Augusta, GA, USA.
authors: Kelli A
authors: KA
authors: Agee
authors: Department of Biomedicine, Unit of Dental Sciences and Biomaterials, University of Trieste, Trieste, Friuli Venezia Giulia, Italy.
authors: Milena
authors: M
authors: Cadenaro
authors: Department of Biomedicine, Unit of Dental Sciences and Biomaterials, University of Trieste, Trieste, Friuli Venezia Giulia, Italy.
authors: Gianluca
authors: G
authors: Turco
authors: Department of Biomedicine, Unit of Dental Sciences and Biomaterials, University of Trieste, Trieste, Friuli Venezia Giulia, Italy.
authors: Lorenzo
authors: L
authors: Breschi
authors: Department of Biomedicine, Unit of Dental Sciences and Biomaterials, University of Trieste, Trieste, Friuli Venezia Giulia, Italy.
authors: Annalisa
authors: A
authors: Mazzoni
authors: Department of Physiology and Pathology, Araraquara School of Dentistry, UNESP - Univ Estadual Paulista, Araraquara, São Paulo, Brazil.
authors: Carlos A de Souza
authors: CA
authors: Costa
authors: Department of Oral Biology, College of Dental Medicine, Georgia Regents University, Augusta, GA, USA. Electronic address: dpashley@georgiahealth.edu.
authors: David H
authors: DH
authors: Pashley
mesh_terms: D002152:Calorimetry, Differential Scanning; D003432:Cross-Linking Reagents / Q000737:chemistry*; D003804:Dentin / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques
chemical_list: D003432:Cross-Linking Reagents
keywords: Collagen; Cross-linkers; Dentin; EDC; Glutaraldehyde; MMPs