title: Mannose-binding lectin genetic analysis: possible protective role of the HYPA haplotype in the development of recurrent urinary tract infections in men.
abstract: Factors related to bacterial virulence and/or to the host have been implicated in the pathogenesis of recurrent urinary tract infections (rUTI), but in most cases the cause is unknown. Mannose binding lectin (MBL) is an acute phase glycoprotein that exerts immunological functions by binding to the surface of a variety of pathogens. Some human gene variants reduce MBL activity thereby predisposing the host to bacterial and viral infections. The aim of this study was to investigate MBL2 gene variants in relation to rUTI risk. Six MBL gene variants and seven haplotypes were analyzed by PCR and direct sequencing in rUTI patients (n = 83) and in healthy subjects from southern Italy (n = 642). The frequencies of the L allele (-550) and the HYPA haplotype were higher in controls than in patients stratified according to sex (p < 0.05). Our data indicate that the HYPA haplotype in the MBL2 gene could be associated with a minor risk of developing rUTI in males.
authors: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Napoli, Italy; CEINGE-Biotecnologie Avanzate, Napoli, Italy. Electronic address: olga.scudiero@unina.it.
authors: Olga
authors: O
authors: Scudiero
authors: CEINGE-Biotecnologie Avanzate, Napoli, Italy; IRCCS-Fondazione SDN, Napoli, Italy.
authors: Maria Ludovica
authors: ML
authors: Monaco
authors: CEINGE-Biotecnologie Avanzate, Napoli, Italy; IRCCS-Fondazione SDN, Napoli, Italy.
authors: Ersilia
authors: E
authors: Nigro
authors: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Napoli, Italy; CEINGE-Biotecnologie Avanzate, Napoli, Italy.
authors: Mario
authors: M
authors: Capasso
authors: Dipartimento di Medicina e Chirurgia, Catterdra di Ginecologia e Ostetricia, Università degli Studi di Salerno, Salerno, Italy.
authors: Maurizio
authors: M
authors: Guida
authors: Dipartimento di Scienze Ostetriche e Ginecologiche, Università degli Studi di Napoli Federico II, Napoli, Italy.
authors: Attilio
authors: A
authors: Di Spiezio Sardo
authors: Dipartimento di Neuroscienze, Scienze Riproduttive e Odontostomatologiche, Università degli Studi di Napoli Federico II, Napoli, Italy.
authors: Domenico
authors: D
authors: Prezioso
authors: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Napoli, Italy; CEINGE-Biotecnologie Avanzate, Napoli, Italy; Dipartimento di Scienze e Tecnologie Ambientali Biologiche Farmaceutiche, Seconda Università degli Studi di Napoli, Caserta, Italy.
authors: Aurora
authors: A
authors: Daniele
authors: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Napoli, Italy; CEINGE-Biotecnologie Avanzate, Napoli, Italy.
authors: Giuseppe
authors: G
authors: Castaldo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D015331:Cohort Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D037601:Mannose-Binding Lectin / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics; D012008:Recurrence; D012306:Risk; D017422:Sequence Analysis, DNA; D012737:Sex Factors; D014552:Urinary Tract Infections / Q000235:genetics*; D055815:Young Adult
chemical_list: D002352:Carrier Proteins; C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin; C491404:PRPF40A protein, human
keywords: Haplotype; MBL; Molecular analysis; rUTI