title: Mast cell deficiency results in the accumulation of preadipocytes in adipose tissue in both obese and non-obese mice.
abstract: Mast cells have been suggested to play key roles in adipogenesis. We herein show that the expression of preadipocyte, but not adipocyte, marker genes increases in the white adipose tissue of mast cell-deficient (Kit(W-sh/W-sh) ) mice under both obese and non-obese conditions. In vitro culturing with adipogenic factors revealed increased adipocytes differentiated from the Kit(W-sh/W-sh) stromal vascular fraction, suggesting the accumulation of preadipocytes. Moreover, the increased expression of preadipocyte genes was restored by mast cell reconstitution in the Kit(W-sh/W-sh) mice. These results suggest positive effects of mast cells on the preadipocyte to adipocyte transition under both physiological and pathological conditions.
authors: Department of Pharmacy, Faculty of Pharmacy, Takasaki University of Health and Welfare, Takasaki, Japan.
authors: Yasushi
authors: Y
authors: Ishijima
authors: Shin'ya
authors: S
authors: Ohmori
authors: Kinuko
authors: K
authors: Ohneda
keywords: Adipogenesis; BMMC, bone marrow-derived mast cell; HFD, high-fat diet; Mast cell; ND, normal diet; Obesity; SVF, stromal vascular fraction; WAT, white adipose tissue; White adipose tissue