title: Walker 256/B malignant breast cancer cells disrupt osteoclast cytomorphometry and activity in rats: modulation by α-tocopherol acetate.
abstract: We examined the effects of vitamin E supplementation (VES) on osteoclast (OC) resorbing activity and cytomorphometry in Walker 256/B tumor osteolytic rats. Twenty-four aged male rats were randomized into 3 groups: 6 were sham operated; 9 were injected in the right hind limb with Walker 256/B cells (W256 group); and 9 were injected as above and supplemented with VE (45mg/kg BW) (W256VE group). Twenty days later, bone mass (BV/TV) and some microarchitectural parameters were assessed. Some histodynamic parameters, cellular and nuclear form factors (FFC and FFN), and nuclear-cytoplasmic ratio (N/C) of OC were measured for each group. W256 group exhibited osteolytic lesions in the operated femora. Walker 256/B induced trabecular perforation and decreased BV/TV associated with significant increases in OC numbering (N.Oc/B.Ar and Oc.N/B.Pm) and activity (ES/BS and Oc.S/BS). While FFN remain unchanged, the FFC and N/C ratio increased in the W256 group. W256VE showed less osteolytic lesions. Moreover, disruption of bone microarchitecture and OC activity in W256VE group decreased. VES reduced the malignant Walker 256/B-induced enhanced OC resorbing activity with cytoinhibition rate reaching 41%. The protective effect of VE may be due to its modulation of OC cytomorphometry and subsequently their activity.
authors: Laboratory of Histology-Embryology, Faculty of Medicine, University of Sfax, 3029 Sfax, Tunisia; Laboratory of Cytology-Histology, Faculty of Medicine, University of Tunis El-Manar, 1007 Tunis, Tunisia. Electronic address: riadh.badraoui@fmsf.rnu.tn.
authors: Riadh
authors: R
authors: Badraoui
authors: Laboratory of Pharmacology, Faculty of Medicine, University of Sfax, 3029 Sfax, Tunisia.
authors: Hmed
authors: H
authors: Ben-Nasr
authors: Laboratory of Histology-Embryology, Faculty of Medicine, University of Sfax, 3029 Sfax, Tunisia.
authors: Selma
authors: S
authors: Amamou
authors: Laboratory of Cytology-Histology, Faculty of Medicine, University of Tunis El-Manar, 1007 Tunis, Tunisia.
authors: Michèle Véronique
authors: MV
authors: El-May
authors: Laboratory of Histology-Embryology, Faculty of Medicine, University of Sfax, 3029 Sfax, Tunisia.
authors: Tarek
authors: T
authors: Rebai
mesh_terms: D000818:Animals; D008297:Male; D051381:Rats; D000135:Acid Phosphatase / Q000378:metabolism; D024502:alpha-Tocopherol / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism; D001859:Bone Neoplasms / Q000378:metabolism / Q000517:prevention & control / Q000556:secondary; D016723:Bone Remodeling / Q000187:drug effects; D019587:Dietary Supplements; D005269:Femur / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007527:Isoenzymes / Q000378:metabolism; D010010:Osteoclasts / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D010014:Osteolysis / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D017207:Rats, Sprague-Dawley; D000071681:Tartrate-Resistant Acid Phosphatase; D013997:Time Factors; D014815:Vitamins / Q000494:pharmacology; D008325:Mammary Neoplasms, Experimental
chemical_list: D000135:Acid Phosphatase; D024502:alpha-Tocopherol; D015415:Biomarkers; D007527:Isoenzymes; D000071681:Tartrate-Resistant Acid Phosphatase; D014815:Vitamins
keywords: Bone metastasis; Osteoclast cytomorphometry; Tumor osteolysis; Walker 256/B breast cancer cells; α-Tocopherol acetate