title: Discovery of a novel class of dimeric Smac mimetics as potent IAP antagonists resulting in a clinical candidate for the treatment of cancer (AZD5582).
abstract: A series of dimeric compounds based on the AVPI motif of Smac were designed and prepared as antagonists of the inhibitor of apoptosis proteins (IAPs). Optimization of cellular potency, physical properties, and pharmacokinetic parameters led to the identification of compound 14 (AZD5582), which binds potently to the BIR3 domains of cIAP1, cIAP2, and XIAP (IC50 = 15, 21, and 15 nM, respectively). This compound causes cIAP1 degradation and induces apoptosis in the MDA-MB-231 breast cancer cell line at subnanomolar concentrations in vitro. When administered intravenously to MDA-MB-231 xenograft-bearing mice, 14 results in cIAP1 degradation and caspase-3 cleavage within tumor cells and causes substantial tumor regressions following two weekly doses of 3.0 mg/kg. Antiproliferative effects are observed with 14 in only a small subset of the over 200 cancer cell lines examined, consistent with other published IAP inhibitors. As a result of its in vitro and in vivo profile, 14 was nominated as a candidate for clinical development.
authors: Oncology iMed, Innovative Medicines & Early Development, AstraZeneca R&D Boston , 35 Gatehouse Drive, Waltham, Massachusetts 02451, United States.
authors: Edward J
authors: EJ
authors: Hennessy
authors: Ammar
authors: A
authors: Adam
authors: Brian M
authors: BM
authors: Aquila
authors: Lillian M
authors: LM
authors: Castriotta
authors: Donald
authors: D
authors: Cook
authors: Maureen
authors: M
authors: Hattersley
authors: Alexander W
authors: AW
authors: Hird
authors: Christopher
authors: C
authors: Huntington
authors: Victor M
authors: VM
authors: Kamhi
authors: Naomi M
authors: NM
authors: Laing
authors: Danyang
authors: D
authors: Li
authors: Terry
authors: T
authors: MacIntyre
authors: Charles A
authors: CA
authors: Omer
authors: Vibha
authors: V
authors: Oza
authors: Troy
authors: T
authors: Patterson
authors: Galina
authors: G
authors: Repik
authors: Michael T
authors: MT
authors: Rooney
authors: Jamal C
authors: JC
authors: Saeh
authors: Li
authors: L
authors: Sha
authors: Melissa M
authors: MM
authors: Vasbinder
authors: Haiyun
authors: H
authors: Wang
authors: David
authors: D
authors: Whitston
mesh_terms: D000480:Alkynes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D019281:Dimerization; D004305:Dose-Response Relationship, Drug; D055808:Drug Discovery; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051379:Mice; D008968:Molecular Conformation; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D023041:Xenograft Model Antitumor Assays
chemical_list: D000480:Alkynes; D000970:Antineoplastic Agents; D051034:Inhibitor of Apoptosis Proteins; C586719:N,N'-(2,2'-(hexa-2,4-diyne-1,6-diylbis(oxy))bis(2,3-dihydro-1H-indene-2,1-diyl))bis(1-(2-cyclohexyl-2-(2-(methylamino)propanamido)acetyl)pyrrolidine-2-carboxamide); D009842:Oligopeptides