title: A family of wheat embryo U2 snRNAs.
abstract: Evidence is presented for the existence of small nuclear RNAs in a higher plant species. Based on subcellular fractionation experiments, wheat embryos contain at least four putative snRNAs, one of which co-migrates on SDS-polyacrylamide gels with a relatively abundant cytoplasmic RNA, W1. We purified W1 from ribosome-free high speed supernatant fractions for characterization studies. Electrophoresis under partially denaturing conditions resolves this RNA into several components which bear m3 (2, 2, 7) G-5' caps and strongly resemble vertebrate U2 snRNA on the basis of modified nucleotide content. Preliminary sequence analyses indicate that wheat embryos contain at least three U2-like RNAs which possess slightly different sequences near their 3' ends.
authors: Department of Botany, University of Minnesota, 55108, St. Paul, MN, U.S.A..
authors: J M
authors: JM
authors: Skuzeski
authors: J J
authors: JJ
authors: Jendrisak