title: Potential of vancomycin for the enantiomeric resolution of FMOC-amino acids by capillary electrophoresis-ion-trap-mass spectrometry.
abstract: The potential of the antibiotic vancomycin (VC) as chiral selector for the enantiomeric separation of amino acids by CE-ESI-MS/MS² was investigated for the first time in this work. Derivatization of amino acids with FMOC-Cl was carried out to enable their interaction with VC as well as the formation of precursor ions with larger m/z which were employed in MS² experiments. The partial filling of a coated capillary was employed to avoid the loss in MS sensitivity originated by the introduction of VC in the ionization source. Under optimized conditions, the simultaneous enantiomeric separation and unequivocal identification of 17 amino acids (two of them being nonprotein amino acids) took place in about 20 min with LODs in the micromolar range.
authors: Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, Faculty of Biology, Environmental Sciences and Chemistry, University of Alcalá, Alcalá de Henares (Madrid), Spain.
authors: Laura
authors: L
authors: Sánchez-Hernández
authors: Elena
authors: E
authors: Domínguez-Vega
authors: Cristina
authors: C
authors: Montealegre
authors: María
authors: M
authors: Castro-Puyana
authors: María Luisa
authors: ML
authors: Marina
authors: Antonio L
authors: AL
authors: Crego
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods*; D005449:Fluorenes / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014640:Vancomycin / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D014640:Vancomycin
keywords: Amino acids; Capillary electrophoresis; Chiral separation; Mass spectrometry; Vancomycin