title: A label-free electrochemiluminescence aptasensor for thrombin detection based on host-guest recognition between tris(bipyridine)ruthenium(II)-β-cyclodextrin and aptamer.
abstract: An ultrasensitive label-free electrochemiluminescence (ECL) aptasensor for the detection of thrombin was developed based on the specific recognition between tris(bipyridine)ruthenium(II)-β-cyclodextrin (tris(bpyRu)-β-CD) and the anti-thrombin aptamer (aptamer). The NH2-aptamer was first immobilized on the activated glassy carbon electrode (GCE) by coupling interaction. By use of the specific recognition between tris(bpyRu)-β-CD and aptamer, tris(bpyRu)-β-CD was then attached on the surface of GCE. Resulting from the outstanding photoactive properties of tris(bpyRu)-β-CD, the fabricated GCE performed strong ECL signal with the coreactant of 2-(dibutylamino)ethanol (DBAE). However, in the presence of thrombin, aptamer-thrombin bioaffinity complexes were formed, which restricted the recognition activities between aptamer and tris(bpyRu)-β-CD. Thus, fewer tris(bpyRu)-β-CD could be attached on the surface of GCE and led to an obvious decrease of ECL signal. Fortunately, the difference of ECL intensity before and after combination with thrombin was logarithmically linear with the concentration of thrombin in a wide range of 10 nM-1 pM. Meantime, a detection limit of 0.1 pM without any other signal labeling or amplifying procedures indicated that the biosensor performed excellent sensitivity, operability and simplicity.
authors: Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China.
authors: Qiong
authors: Q
authors: Chen
authors: Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China.
authors: Hong
authors: H
authors: Chen
authors: Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China.
authors: Yingying
authors: Y
authors: Zhao
authors: Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China.
authors: Fan
authors: F
authors: Zhang
authors: Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China.
authors: Fan
authors: F
authors: Yang
authors: Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China.
authors: Jie
authors: J
authors: Tang
authors: Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China. Electronic address: pghe@chem.ecnu.edu.cn.
authors: Pingang
authors: P
authors: He
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D056831:Coordination Complexes; D055664:Electrochemical Techniques / Q000379:methods; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D012680:Sensitivity and Specificity; D013917:Thrombin / Q000032:analysis*; D047392:beta-Cyclodextrins / Q000737:chemistry*; D017975:Ruthenium Compounds
chemical_list: D015082:2,2'-Dipyridyl; D052157:Aptamers, Nucleotide; D056831:Coordination Complexes; D013917:Thrombin; D047392:beta-Cyclodextrins; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds; C081676:thrombin aptamer; C031215:betadex
keywords: Aptasensor; Electrochemiluminescence; Host–guest recognition; Tris(bipyridine)ruthenium(II)-β-cyclodextrin