title: Adaptation strategies of two closely related Desmodesmus armatus (green alga) strains contained different amounts of cadmium: a study with light-induced synchronized cultures of algae.
abstract: During the Desmodesmus armatus cell cycle, 8-celled coenobia of 276-4d strain accumulated a much lower amounts of cadmium than unicells of B1-76 strain. Cadmium reduced growth and photosynthesis in the cells of strain B1-76, but not those of 276-4d strain. Cells of 276-4d strain revealed a higher activity of superoxide dismutase (SOD) isoforms, in particular the activity and protein content of Fe-SOD. Cu/Zn-SOD was earlier and much stronger induced by cadmium in 276-4d than in B1-76 strain, whereas Fe- and Mn-SOD activity and Fe-SOD synthesis were induced only in 276-4d strain. Cadmium did not affect the heat shock protein 70 synthesis in B1-76 strain, but significantly stimulated this process in 276-4d strain. The level of glutathione increased 30-fold during cell development of Cd-exposed 276-4d strain, while in B1-76 it increased about 12 timed. Matured cells of both strains exposed to cadmium produced comparable amounts of phytochelatins and other thiol peptides, but their production in young cells of B1-76 strain was much higher than in 276-4d strain. In conclusion, a complex of internal detoxification mechanisms appeared to be more efficient in cells of 276-4d strain than B1-76 one.
authors: Department of Plant Physiology and Biotechnology, University of Gdańsk, ul. Wita Stwosza 59, 80-308 Gdańsk, Poland.
authors: Wojciech
authors: W
authors: Pokora
authors: Department of Plant Physiology and Biotechnology, University of Gdańsk, ul. Wita Stwosza 59, 80-308 Gdańsk, Poland.
authors: Agnieszka
authors: A
authors: Baścik-Remisiewicz
authors: Department of Plant Physiology and Biotechnology, University of Gdańsk, ul. Wita Stwosza 59, 80-308 Gdańsk, Poland.
authors: Stefan
authors: S
authors: Tukaj
authors: PAS Centre for Ecological Research, Experimental Station, ul. Niecała 18, 20-080 Lublin, Poland.
authors: Renata
authors: R
authors: Kalinowska
authors: Department of Hydrobiology, University of Life Sciences, ul. Dobrzańskiego 37, 20-262 Lublin, Poland.
authors: Barbara
authors: B
authors: Pawlik-Skowrońska
authors: Department of Toxicology, Medical University of Gdansk, Al. Gen. Hallera, 107, 80-416 Gdańsk, Poland.
authors: Małgorzata
authors: M
authors: Dziadziuszko
authors: Department of Plant Physiology and Biotechnology, University of Gdańsk, ul. Wita Stwosza 59, 80-308 Gdańsk, Poland. Electronic address: biozt@ug.edu.pl.
authors: Zbigniew
authors: Z
authors: Tukaj
mesh_terms: D000220:Adaptation, Biological; D002104:Cadmium / Q000378:metabolism*; D002734:Chlorophyll / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D008027:Light; D010100:Oxygen / Q000378:metabolism; D010788:Photosynthesis; D054811:Phytochelatins / Q000378:metabolism; D044444:Scenedesmus / Q000378:metabolism*; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D018840:HSP70 Heat-Shock Proteins; D002104:Cadmium; D002734:Chlorophyll; D054811:Phytochelatins; D013482:Superoxide Dismutase; D005978:Glutathione; D010100:Oxygen
keywords: Adaptation strategy; Cadmium; Cell cycle; Desmodesmus; GSH; HSP; PC; PMSF; ROS; SOD; glutathione; heat shock protein; phenylmethanesulfonyl fluoride; phytochelatins; reactive oxygen species; superoxide dismutase