title: Comparative genomic analysis shows that Streptococcus suis meningitis isolate SC070731 contains a unique 105K genomic island.
abstract: Streptococcus suis (SS) is an important swine pathogen worldwide that occasionally causes serious infections in humans. SS infection may result in meningitis in pigs and humans. The pathogenic mechanisms of SS are poorly understood. Here, we provide the complete genome sequence of S. suis serotype 2 (SS2) strain SC070731 isolated from a pig with meningitis. The chromosome is 2,138,568bp in length. There are 1933 predicted protein coding sequences and 96.7% (57/59) of the known virulence-associated genes are present in the genome. Strain SC070731 showed similar virulence with SS2 virulent strains HA9801 and ZY05719, but was more virulent than SS2 virulent strain P1/7 in the zebrafish infection model. Comparative genomic analysis revealed a unique 105K genomic island in strain SC070731 that is absent in seven other sequenced SS2 strains. Further analysis of the 105K genomic island indicated that it contained a complete nisin locus similar to the nisin U locus in S. uberis strain 42, a prophage similar to S. oralis phage PH10 and several antibiotic resistance genes. Several proteins in the 105K genomic island, including nisin and RelBE toxin-antitoxin system, contribute to the bacterial fitness and virulence in other pathogenic bacteria. Further investigation of newly identified gene products, including four putative new virulence-associated surface proteins, will improve our understanding of SS pathogenesis.
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing 210095, China; OIE Reference Lab for Swine Streptococcosis, Nanjing 210095, China. Electronic address: wuzongfu@njau.edu.cn.
authors: Zongfu
authors: Z
authors: Wu
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing 210095, China; OIE Reference Lab for Swine Streptococcosis, Nanjing 210095, China.
authors: Weixue
authors: W
authors: Wang
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing 210095, China; OIE Reference Lab for Swine Streptococcosis, Nanjing 210095, China.
authors: Min
authors: M
authors: Tang
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing 210095, China; OIE Reference Lab for Swine Streptococcosis, Nanjing 210095, China.
authors: Jing
authors: J
authors: Shao
authors: Experimental Teaching Center of Life Science, Nanjing Agricultural University, Nanjing 210095, China.
authors: Chen
authors: C
authors: Dai
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing 210095, China; OIE Reference Lab for Swine Streptococcosis, Nanjing 210095, China.
authors: Wei
authors: W
authors: Zhang
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing 210095, China; OIE Reference Lab for Swine Streptococcosis, Nanjing 210095, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
authors: Hongjie
authors: H
authors: Fan
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing 210095, China; OIE Reference Lab for Swine Streptococcosis, Nanjing 210095, China.
authors: Huochun
authors: H
authors: Yao
authors: Novel Bioinformatics Co., Ltd, Shanghai, China.
authors: Jie
authors: J
authors: Zong
authors: Novel Bioinformatics Co., Ltd, Shanghai, China.
authors: Dai
authors: D
authors: Chen
authors: Novel Bioinformatics Co., Ltd, Shanghai, China.
authors: Junning
authors: J
authors: Wang
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing 210095, China; OIE Reference Lab for Swine Streptococcosis, Nanjing 210095, China. Electronic address: lucp@njau.edu.cn.
authors: Chengping
authors: C
authors: Lu
mesh_terms: D000818:Animals; D002876:Chromosomes, Bacterial; D055028:Comparative Genomic Hybridization; D023061:Gene Order; D016680:Genome, Bacterial; D044404:Genomic Islands; D023281:Genomics; D006801:Humans; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D010641:Phenotype; D039002:Prophages / Q000235:genetics; D017011:Streptococcus suis / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D013552:Swine; D014774:Virulence / Q000235:genetics
keywords: CDS; CSF; GI; Genomic island; HTP; Histidine triad proteins; IS; LC–MS; Nisin; PI; Phage; SS2; STSS; Streptococcus suis serotype 2; TA; Virulence; cerebrospinal fluid; coding sequences; genomic island; insertion sequences; liquid chromatography–mass spectrometry; pathogenicity island; streptococcal toxic shock syndrome; toxin–antitoxin