title: Inhibition of biomass activity in the via nitrite nitrogen removal processes by veterinary pharmaceuticals.
abstract: The inhibitory effect of two veterinary pharmaceuticals was studied for different types of biomass involved in via nitrite nitrogen removal processes. Batch tests were conducted to determine the inhibition level of acetaminophen (PAR) and doxycycline (DOX) on the activity of short-cut nitrifying, denitrifying and anoxic ammonium oxidation (anammox) biomass and phosphorus accumulating organisms (PAOs). All biomass types were affected by PAR and DOX, with anammox being the most sensitive bacteria. DOX inhibited more the biomass treating high strength nitrogenous effluents (HSNE) than low strength nitrogenous effluents (LSNE). The phosphorus uptake inhibition under anoxic conditions was lower than 25% in the presence of PAR up to 400 mg L(-1). The same DOX concentration inhibited anoxic phosphorus uptake more than 65% for biomass treating LSNE and HSNE. Heterotrophic denitrifying bacteria seem to be more robust at high DOX and PAR concentrations than anammox. Both veterinary products inactivated ammonium oxidizing, Accumulibacter phosphatis and denitrifying bacteria.
authors: Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, E-15782 Santiago de Compostela, Spain. Electronic address: teresa.alvarino@usc.es.
authors: Teresa
authors: T
authors: Alvarino
authors: Department of Biotechnology, University of Verona, Strada Le Grazie 15, I-37134 Verona, Italy. Electronic address: evangelia.katsou@univr.it.
authors: Evina
authors: E
authors: Katsou
authors: Department of Biotechnology, University of Verona, Strada Le Grazie 15, I-37134 Verona, Italy. Electronic address: malamis.simos@gmail.com.
authors: Simos
authors: S
authors: Malamis
authors: Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, E-15782 Santiago de Compostela, Spain. Electronic address: sonia.suarez@usc.es.
authors: Sonia
authors: S
authors: Suarez
authors: Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, E-15782 Santiago de Compostela, Spain. Electronic address: francisco.omil@usc.es.
authors: Francisco
authors: F
authors: Omil
authors: Department of Biotechnology, University of Verona, Strada Le Grazie 15, I-37134 Verona, Italy. Electronic address: franceso.fatone@univr.it.
authors: Francesco
authors: F
authors: Fatone
mesh_terms: D000082:Acetaminophen / Q000302:isolation & purification / Q000494:pharmacology; D064751:Ammonium Compounds / Q000378:metabolism; D001419:Bacteria / Q000187:drug effects; D001673:Biodegradation, Environmental / Q000187:drug effects; D018533:Biomass; D004318:Doxycycline / Q000302:isolation & purification / Q000494:pharmacology; D017404:In Situ Hybridization, Fluorescence; D020128:Inhibitory Concentration 50; D009573:Nitrites / Q000302:isolation & purification* / Q000378:metabolism; D009584:Nitrogen / Q000302:isolation & purification* / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D012722:Sewage / Q000382:microbiology; D019155:Veterinary Drugs / Q000302:isolation & purification / Q000494:pharmacology*; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D064751:Ammonium Compounds; D009573:Nitrites; D012722:Sewage; D019155:Veterinary Drugs; D010758:Phosphorus; D000082:Acetaminophen; D004318:Doxycycline; D009584:Nitrogen; D010100:Oxygen
keywords: Activity inhibition; Anammox; FISH; Short-cut nutrients removal; Veterinary pharmaceuticals