title: Safety of the intravenous administration of neurotensin-polyplex nanoparticles in BALB/c mice.
abstract: Neurotensin (NTS)-polyplex is a gene nanocarrier that has potential nanomedicine-based applications for the treatment of Parkinson's disease and cancers of cells expressing NTS receptor type 1. We assessed the acute inflammatory response to NTS-polyplex carrying a reporter gene in BALB/c mice. The intravenous injection of NTS-polyplex caused the specific expression of the reporter gene in gastrointestinal cells. Six hours after an intravenous injection of propidium iodide labeled-NTS-polyplex, fluorescent spots were located in the cells of the organs with a mononuclear phagocyte system, suggesting NTS-polyplex clearance. In contrast to lipopolysaccharide and carbon tetrachloride, NTS-polyplex did not increase the serum levels of tumor necrosis factor alpha, interleukin (IL)-1β, IL-6, bilirubin, aspartate transaminase, and alanine transaminase. NTS-polyplex increased the levels of serum amyloid A and alkaline phosphatase, but these levels normalized after 24 h. Compared to carrageenan, the local injection of NTS-polyplex did not produce inflammation. Our results support the safety of NTS-polyplex.
  
  
  BACKGROUND
  This study focuses on the safety of neurotensin (NTS)-polyplex, a gene nanocarrier that has potential in the treatment of Parkinson's disease and cancers of cells expressing NTS receptor type 1. NTS polyplex demonstrates a better safety profile compared with carrageenan, lipopolysaccharide, and carbon tetrachloride in a murine model.
authors: Department of Psychoimmunology, INPRF, Mexico DF, Mexico.
authors: Maria E
authors: ME
authors: Hernandez
authors: Department of Neuropathology, INNN, Mexico DF, Mexico.
authors: Jesus D
authors: JD
authors: Rembao
authors: Department of Physiology, Biophysics and Neurosciences, CINVESTAV-I.P.N., Mexico DF, Mexico.
authors: Daniel
authors: D
authors: Hernandez-Baltazar
authors: Department of Physiology, Biophysics and Neurosciences, CINVESTAV-I.P.N., Mexico DF, Mexico.
authors: Rosa A
authors: RA
authors: Castillo-Rodriguez
authors: Department of Physiology, Biophysics and Neurosciences, CINVESTAV-I.P.N., Mexico DF, Mexico.
authors: Victor M
authors: VM
authors: Tellez-Lopez
authors: Department of Physiology, Biophysics and Neurosciences, CINVESTAV-I.P.N., Mexico DF, Mexico.
authors: Yazmin M
authors: YM
authors: Flores-Martinez
authors: Department of Physiology, Biophysics and Neurosciences, CINVESTAV-I.P.N., Mexico DF, Mexico.
authors: Carlos E
authors: CE
authors: Orozco-Barrios
authors: School of Medicine, UADY, Merida, Yucatan, Mexico.
authors: Hector A
authors: HA
authors: Rubio
authors: Department of Neuropathology, INNN, Mexico DF, Mexico.
authors: Aurora
authors: A
authors: Sánchez-García
authors: Department of Physiology, Biophysics and Neurosciences, CINVESTAV-I.P.N., Mexico DF, Mexico.
authors: Jose
authors: J
authors: Ayala-Davila
authors: Instituto de Ciencias, Facultad de Medicina Clínica Alemana Universidad del Desarrollo, Santiago, Chile.
authors: Martha L
authors: ML
authors: Arango-Rodriguez
authors: Department of Psychoimmunology, INPRF, Mexico DF, Mexico.
authors: Lenin
authors: L
authors: Pavón
authors: Department of Physiology, Biophysics and Neurosciences, CINVESTAV-I.P.N., Mexico DF, Mexico.
authors: Teresa
authors: T
authors: Mejia-Castillo
authors: UMR_S938, Hôpital Saint-Antoine, Paris Cedex 12, France.
authors: Patricia
authors: P
authors: Forgez
authors: Department of Physiology, Biophysics and Neurosciences, CINVESTAV-I.P.N., Mexico DF, Mexico; PhD Program in Nanoscience and Nanotechnology; CINVESTAV-I.P.N., Mexico DF, Mexico. Electronic address: dmartine@fisio.cinvestav.mx.
authors: Daniel
authors: D
authors: Martinez-Fong
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D051379:Mice; D053758:Nanoparticles; D010300:Parkinson Disease / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D018028:Receptors, Neurotensin / Q000096:biosynthesis; D012449:Safety
chemical_list: D018028:Receptors, Neurotensin; C114036:neurotensin type 1 receptor
keywords: Biosafety; Cytotoxicity; Nanomaterials; Pro-inflammatory cytokines