title: Control of cell adhesion and detachment on Langmuir-Schaefer surface composed of dodecyl-terminated thermo-responsive polymers.
abstract: This study used Langmuir-Schaefer (LS) method to produce thermo-responsive poly(N-isopropylacrylamide) (PIPAAm) modified surface. Dodecyl terminated-PIPAAm (PIPAAm-C12) was synthesized by reversible addition-fragmentation chain transfer radical polymerization. PIPAAm-C12 was dropped on an air-water interface and formed Langmuir film by compressing. A surface pressure measurement revealed that PIPAAm-C12 was floated and Langmuir films were formed on the interface. And the Langmuir film was transferred on a hydrophobic substrate to produce PIPAAm-C12 transferred surface (PIPAAm-LS surface). In the results of atomic force microscope, attenuated total reflection Fourier transform infrared spectroscope, and X-ray photoelectron spectroscope measurement, the transference of Langmuir films was demonstrated and densities could be precisely controlled. Ellipsometric measurements of PIPAAm-LS surfaces showed that the thicknesses of the surfaces were less than 10 nm. Cell adhesion and detachment were observed on the PIPAAm-LS surfaces. The amount of adhered cells on all LS surfaces was found to be similar on the control hydrophobic substrate at 37 °C. In regard to cell detachment, adhering cells rapidly detached themselves with higher densities and shorter PIPAAm-C12 molecules. In this method, the effect of densities and molecular weights on cell adhesion and detachment were observed. Our method should be proved novel insights for investigating cell adhesion and detachment on thermo-responsive surfaces.
authors: a Faculty of Science, Department of Physics , Tokyo University of Science , 1-3 Kagurazaka, Shinjuku, Tokyo 162-8601 , Japan.
authors: Morito
authors: M
authors: Sakuma
authors: Yoshikazu
authors: Y
authors: Kumashiro
authors: Masamichi
authors: M
authors: Nakayama
authors: Nobuyuki
authors: N
authors: Tanaka
authors: Kazuo
authors: K
authors: Umemura
authors: Masayuki
authors: M
authors: Yamato
authors: Teruo
authors: T
authors: Okano
mesh_terms: D000180:Acrylic Resins / Q000138:chemical synthesis / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D002339:Carotid Arteries / Q000166:cytology*; D002417:Cattle; D002448:Cell Adhesion; D002460:Cell Line; D042783:Endothelial Cells / Q000166:cytology*; D057927:Hydrophobic and Hydrophilic Interactions; D056951:Photoelectron Spectroscopy; D058105:Polymerization; D013499:Surface Properties; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; C052970:poly-N-isopropylacrylamide