title: Molecular phylogeny and barcoding of Caulerpa (Bryopsidales) based on the tufA, rbcL, 18S rDNA and ITS rDNA genes.
abstract: The biodiversity assessment of different taxa of the genus Caulerpa is of interest from the context of morphological plasticity, invasive potential of some species and biotechnological and pharmacological applications. The present study investigated the identification and molecular phylogeny of different species of Caulerpa occurring along the Indian coast inferred from tufA, rbcL, 18S rDNA and ITS rDNA nucleotide sequences. Molecular data confirmed the identification of 10 distinct Caulerpa species: C. veravalensis, C. verticillata, C. racemosa, C. microphysa, C. taxifolia, C. sertularioides, C. scalpelliformis, C. serrulata, C. peltata and C. mexicana. All datasets significantly supported the sister relationship between C. veravalensis and C. racemosa var. cylindracea. It was also concluded from the results that the specimen identified previously as C. microphysa and C. lentillifera could not be considered as separate species. The molecular data revealed the presence of multiple lineages for C. racemosa which can be resolved into separate species. All four markers were used to ascertain their utility for DNA barcoding. The tufA gene proved a better marker with monophyletic association as the main criteria for identification at the species level. The results also support the use of 18S rDNA insertion sequences to delineate the Caulerpa species through character-based barcoding. The ITS rDNA (5.8S-ITS2) phylogenetic analysis also served as another supporting tool. Further, more sequences from additional Caulerpa specimens will need to be analysed in order to support the role of these two markers (ITS rDNA and 18S insertion sequence) in identification of Caulerpa species. The present study revealed the phylogeny of Caulerpa as complete as possible using the currently available data, which is the first comprehensive report illustrating the molecular phylogeny and barcoding of the genus Caulerpa from Indian waters.
authors: Discipline of Marine Biotechnology and Ecology, CSIR- Central Salt and Marine Chemicals Research Institute, Bhavnagar, Gujarat, India ; Academy of Scientific and Innovative Research (AcSIR), CSIR, New Delhi, India.
authors: Mudassar Anisoddin
authors: MA
authors: Kazi
authors: C R K
authors: CR
authors: Reddy
authors: Bhavanath
authors: B
authors: Jha
mesh_terms: D046068:Caulerpa / Q000235:genetics*; D058893:DNA Barcoding, Taxonomic; D018744:DNA, Plant / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D024101:Mitochondrial Proteins / Q000235:genetics*; D010444:Peptide Elongation Factor Tu / Q000235:genetics*; D010802:Phylogeny; D018749:RNA, Plant / Q000235:genetics*; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D004275:DNA, Ribosomal; D024101:Mitochondrial Proteins; D018749:RNA, Plant; D012337:RNA, Ribosomal, 18S; D010444:Peptide Elongation Factor Tu; C101625:RbcL protein, plastid; D012273:Ribulose-Bisphosphate Carboxylase