title: Quick-response magnetic nanospheres for rapid, efficient capture and sensitive detection of circulating tumor cells.
abstract: The study on circulating tumor cells (CTCs) has great significance for cancer prognosis, treatment monitoring, and metastasis diagnosis, in which isolation and enrichment of CTCs are key steps due to their extremely low concentration in peripheral blood. Herein, magnetic nanospheres (MNs) were fabricated by a convenient and highly controllable layer-by-layer assembly method. The MNs were nanosized with fast magnetic response, and nearly all of the MNs could be captured by 1 min attraction with a commercial magnetic scaffold. In addition, the MNs were very stable without aggregation or precipitation in whole blood and could be re-collected nearly at 100% in a monodisperse state. Modified with anti-epithelial-cell-adhesion-molecule (EpCAM) antibody, the obtained immunomagnetic nanospheres (IMNs) successfully captured extremely rare tumor cells in whole blood with an efficiency of more than 94% via only a 5 min incubation. Moreover, the isolated cells remained viable at 90.5 ± 1.2%, and they could be directly used for culture, reverse transcription-polymerase chain reaction (RT-PCR), and immunocytochemistry (ICC) identification. ICC identification and enumeration of the tumor cells in the same blood samples showed high sensitivity and good reproducibility. Furthermore, the IMNs were successfully applied to the isolation and detection of CTCs in cancer patient peripheral blood samples, and even one CTC in the whole blood sample was able to be detected, which suggested they would be a promising tool for CTC enrichment and detection.
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, and Wuhan Institute of Biotechnology, Wuhan University , Wuhan 430072, People's Republic of China.
authors: Cong-Ying
authors: CY
authors: Wen
authors: Ling-Ling
authors: LL
authors: Wu
authors: Zhi-Ling
authors: ZL
authors: Zhang
authors: Yu-Lin
authors: YL
authors: Liu
authors: Shao-Zhong
authors: SZ
authors: Wei
authors: Jiao
authors: J
authors: Hu
authors: Man
authors: M
authors: Tang
authors: En-Ze
authors: EZ
authors: Sun
authors: Yi-Ping
authors: YP
authors: Gong
authors: Jing
authors: J
authors: Yu
authors: Dai-Wen
authors: DW
authors: Pang
mesh_terms: D000951:Antigens, Neoplasm / Q000276:immunology; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000276:immunology; D045744:Cell Line, Tumor; D017931:DNA Primers; D000071858:Epithelial Cell Adhesion Molecule; D006801:Humans; D018189:Immunomagnetic Separation; D046529:Microscopy, Electron, Transmission; D054118:Nanospheres; D009369:Neoplasms / Q000097:blood*; D009360:Neoplastic Cells, Circulating; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D017931:DNA Primers; C499379:EPCAM protein, human; D000071858:Epithelial Cell Adhesion Molecule