title: BcMF21 is important for pollen development and germination in Brassica campestris ssp. chinensis.
abstract: Brassica campestris Male Fertility 21 (BcMF21) was previously isolated from the flower buds of Chinese cabbage (Brassica campestris L. ssp. chinensis Makino, syn. B. rapa ssp. chinensis) and expressed specifically in tapetum and microspores during the meiosis stage and the uninucleate stage of microspore development. Here, we used antisense RNA technology to knock down the expression level of BcMF21 in B. campestris and analyzed the phenotype of the transgenic plants. Alexander staining and scanning electron microscope revealed sterility and exine deformities in the mature pollen grains of BcMF21 antisense RNA transgenic plants. The germination furrow of the BcMF21 antisense RNA transgenic pollen was covered by lipid like materials. The pollen tubes burst and could not grow normally in vitro. Therefore, we presented here BcMF21 might be an important gene for pollen development and germination.
authors: Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, China, jingjingjiang@zju.edu.cn.
authors: Jingjing
authors: J
authors: Jiang
authors: Youjian
authors: Y
authors: Yu
authors: Heng
authors: H
authors: Dong
authors: Lina
authors: L
authors: Yao
authors: Zhixian
authors: Z
authors: Zhang
authors: Jiashu
authors: J
authors: Cao
mesh_terms: D029741:Brassica rapa / Q000254:growth & development* / Q000378:metabolism; D055785:Gene Knockdown Techniques; D018525:Germination; D051479:Plant Infertility / Q000235:genetics; D010940:Plant Proteins / Q000502:physiology*; D011058:Pollen / Q000254:growth & development* / Q000378:metabolism; D016372:RNA, Antisense / Q000235:genetics
chemical_list: D010940:Plant Proteins; D016372:RNA, Antisense