title: Cell-free translation of exogenous mRNA in extracts from dry pea primary axes.
abstract: Extracts from the primary axes of dry pea (Pisum sativum L.) seeds are able to perform an initiation-dependent translation of exogenous mRNA. SDS polyacrylamide gel electrophoresis of the products synthesized under direction of alfalfa mosaic virus RNA (AMV-RNA) and tobacco mosaic virus RNA (TMV-RNA) shows that the fidelity of translation in this pea system is at least as high as in a wheat embryo cell-free protein synthesizing system. The endogenous messengers are also efficiently translated in extracts from the primary axes of pea seeds. The direct translation of these messengers in a homologous cell-free system may be of interest for a study of the products coded for by the long-lived messengers present in this plant.
authors: Laboratorium voor Plantenbiochemie, Katholieke Universiteit Leuven, Vaartstraat 24, B-3000, Leuven, Belgium.
authors: W J
authors: WJ
authors: Peumans
authors: A R
authors: AR
authors: Carlier
authors: J
authors: J
authors: Schreurs