title: Isolation of Bacillus sp. strains capable of decomposing alkali lignin and their application in combination with lactic acid bacteria for enhancing cellulase performance.
abstract: Effective biological pretreatment method for enhancing cellulase performance was investigated. Two alkali lignin-degrading bacteria were isolated from forest soils in Japan and named CS-1 and CS-2. 16S rDNA sequence analysis indicated that CS-1 and CS-2 were Bacillus sp. Strains CS-1 and CS-2 displayed alkali lignin degradation capability. With initial concentrations of 0.05-2.0 g L(-1), at least 61% alkali lignin could be degraded within 48 h. High laccase activities were observed in crude enzyme extracts from the isolated strains. This result indicated that alkali lignin degradation was correlated with laccase activities. Judging from the net yields of sugars after enzymatic hydrolysis, the most effective pretreatment method for enhancing cellulase performance was a two-step processing procedure (pretreatment using Bacillus sp. CS-1 followed by lactic acid bacteria) at 68.6%. These results suggest that the two-step pretreatment procedure is effective at accelerating cellulase performance.
authors: Division of Applied Sciences, College of Environmental Technology, Graduate School of Engineering, Muroran Institute of Technology, 27-1 Mizumoto, Muroran 050-8585, Hokkaido, Japan. Electronic address: ychang@mmm.muroran-it.ac.jp.
authors: Young-Cheol
authors: YC
authors: Chang
authors: Department of Pharmacy, College of Pharmacy, Chungbuk National University, Cheongju 361-763, Republic of Korea. Electronic address: choidubok@naver.com.
authors: Dubok
authors: D
authors: Choi
authors: Department of Applied Life Science, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.
authors: Kazuhiro
authors: K
authors: Takamizawa
authors: Division of Applied Sciences, College of Environmental Technology, Graduate School of Engineering, Muroran Institute of Technology, 27-1 Mizumoto, Muroran 050-8585, Hokkaido, Japan.
authors: Shintaro
authors: S
authors: Kikuchi
mesh_terms: D000468:Alkalies / Q000378:metabolism*; D000880:Anthraquinones / Q000302:isolation & purification; D001407:Bacillus / Q000302:isolation & purification* / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002480:Cellulase / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D042845:Laccase / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism*; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry; D012988:Soil Microbiology; D012995:Solubility; D013997:Time Factors; D014866:Waste Products
chemical_list: D000468:Alkalies; D000880:Anthraquinones; D014866:Waste Products; D019344:Lactic Acid; D008031:Lignin; D042845:Laccase; D002480:Cellulase; D005947:Glucose; C024758:Remazol Brilliant Blue R
keywords: Bacillus sp.; Cellulase performance; Laccase; Lactic acid bacteria; Lignin-degradation