title: Evaluation of stem cell marker gene expression in canine prostate carcinoma- and prostate cyst-derived cell lines.
abstract: OBJECTIVE
  In human prostate cancer cells with a stem cell-like character (cancer stem cells, CSC) are considered to play a major role in disease development, progression and relapse. Aim of the study was to evaluate if similar cells are present and active in canine prostate cancer providing a naturally-occurring mammalian model for the development of therapeutic approaches targeting CSC.
  
  
  METHODS
  Stem cell marker expression of CD133, CD44, C-KIT, CD34, ITGA6, OCT4, DDX5 and MELK in canine prostate carcinomas and prostate cyst cell lines were screened by Polymerase Chain Reaction (PCR), quantitative Polymerase Chain Reaction (qPCR) and partially analysed by flow cytometry.
  
  
  RESULTS
  Marker analyses by PCR and qPCR, revealed a complex expression pattern for the analysed marker genes, providing a characteristic marker pattern for the studied cell lines. Thereby CD44, CD133, ITGA6 and DDX5 showed the most prominent expression in the analysed cell lines.
  
  
  CONCLUSIONS
  The results revealed a characteristic stem cell marker expression in the analysed cell lines, indicating the presence of CSC in canine prostate cancer.
authors: Prof. Dr., Small Animal Clinic, University of Veterinary Medicine Hannover, Bünteweg 9, 30550 Hannover, Germany. ingo.nolte@tiho-hannover.de.
authors: Mohammed
authors: M
authors: Moulay
authors: Wen
authors: W
authors: Liu
authors: Saskia
authors: S
authors: Willenbrock
authors: Katharina Anna
authors: KA
authors: Sterenczak
authors: Regina
authors: R
authors: Carlson
authors: Anaclet
authors: A
authors: Ngezahayo
authors: Hugo
authors: H
authors: Murua Escobar
authors: Ingo
authors: I
authors: Nolte
mesh_terms: D000818:Animals; D001483:Base Sequence; D015415:Biomarkers / Q000378:metabolism*; D002460:Cell Line; D003560:Cysts / Q000235:genetics / Q000473:pathology / Q000662:veterinary; D017931:DNA Primers; D004285:Dogs; D015870:Gene Expression; D008297:Male; D011469:Prostatic Diseases / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D060888:Real-Time Polymerase Chain Reaction; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D017931:DNA Primers
keywords: CT1258; CT1258-EGFP; CT1258-EGFP-HMGA2 cell lines; Prostate cancer; cancer stem cells; canine; stem cell marker