title: PCR assays for the identification of rare recombination types from VP1 to 3D genomic region of vaccine derived poliovirus strains.
abstract: Poliomyelitis has been effectively controlled by the use of inactivated poliovirus vaccine (IPV) or trivalent live attenuated oral poliovirus vaccine (OPV). Since 1964, the use of OPV in mass vaccinations has resulted in drastic reductions of the number of poliomyelitis cases caused by wild-type polioviruses. However, the characterization of OPV derivatives with increased neurovirulence, constituted a real problem with respect to OPV safety. Mutations at attenuating sites of the genome and recombination events between Sabin strains of the trivalent OPV vaccine have been correlated with the loss of the attenuated phenotype of OPV strains and the acquisition of traits characteristic of wild polioviruses. In consequence, early detection and characterization of recombinant evolved derivatives of vaccine strains is highly important. In this report, ten PCR assays are described which allow for the identification of rare recombination events located in VP1, 2A, 2C, 3A, 3C and 3D genomic regions and predominant recombination events located in 2C and 3D genomic regions of OPV derivatives. These assays could be readily implemented in diagnostics laboratories lacking sequencing facilities as a first approach for the early detection and characterization of recombinant OPV derivatives.
authors: University of Thessaly, School of Health Sciences, Department of Biochemistry & Biotechnology, Microbiology-Virology Laboratory, Larissa, Greece.
authors: T G
authors: TG
authors: Dimitriou
authors: University of Thessaly, School of Health Sciences, Department of Biochemistry & Biotechnology, Microbiology-Virology Laboratory, Larissa, Greece.
authors: V
authors: V
authors: Pliaka
authors: University of Thessaly, School of Health Sciences, Department of Biochemistry & Biotechnology, Microbiology-Virology Laboratory, Larissa, Greece.
authors: Z
authors: Z
authors: Kyriakopoulou
authors: University of Thessaly, School of Health Sciences, Department of Biochemistry & Biotechnology, Microbiology-Virology Laboratory, Larissa, Greece.
authors: I G A
authors: IG
authors: Ruether
authors: University of Thessaly, School of Health Sciences, Department of Biochemistry & Biotechnology, Microbiology-Virology Laboratory, Larissa, Greece.
authors: D
authors: D
authors: Tsakogiannis
authors: University of Thessaly, School of Health Sciences, Department of Biochemistry & Biotechnology, Microbiology-Virology Laboratory, Larissa, Greece.
authors: P
authors: P
authors: Fountoucidou
authors: University of Ioannina, Medical School, Department of Microbiology, Ioannina, Greece.
authors: C
authors: C
authors: Gartzonika
authors: University of Ioannina, Medical School, Department of Microbiology, Ioannina, Greece.
authors: S
authors: S
authors: Levidiotou-Stefanou
authors: University of Thessaly, School of Health Sciences, Department of Biochemistry & Biotechnology, Microbiology-Virology Laboratory, Larissa, Greece. Electronic address: markoulatos@bio.uth.gr.
authors: P
authors: P
authors: Markoulatos
mesh_terms: D016679:Genome, Viral; D006801:Humans; D011051:Poliomyelitis / Q000517:prevention & control; D011055:Poliovirus Vaccine, Oral / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis; D011995:Recombination, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D011055:Poliovirus Vaccine, Oral; D012367:RNA, Viral
keywords: Characterization of recombination types; OPV derivatives; PCR detection; Rare recombinants