title: Separation and enrichment of major quinolizidine type alkaloids from Sophora alopecuroides using macroporous resins.
abstract: Matrine (MT), oxymatrine (OM) and sophoridine (SP) are three bioactive alkaloids in Sophora alopecuroides. In the present study, the chromatographic characteristics of six widely used macroporous resins, namely NKA, NKA-9, HPD-100, HPD-722, HPD-750, and AB-8, respectively, towards the separation and enrichment of the three alkaloids from the aqueous extract of S. alopecuroides are critically evaluated. The results indicated that AB-8 resin offered the best absorption and desorption capacity and its adsorption data fitted best to the Freundlich isotherm. Dynamic adsorption and desorption experiments on packed columns of AB-8 resin have been investigated for optimization of chromatographic parameters. The adsorption of the alkaloids on the resin was best achieved by 5 bed volume (BV) of sample solution of pH 10 with a flow rate of 2BV/h. The desorption of the compounds from the resin was effectively completed by using 5BV of 80% ethanol in water at a flow rate of 2BV/h. After one run of adsorption and desorption, the contents of MT, OM, and SP were increased from 9.30, 8.39 and 9.84% to 22.22, 21.44 and 28.02%, the recovery were 69.4, 78.3 and 72.6%, respectively. This method would provide useful information to the industrial production of the alkaloids from S. alopecuroides.
authors: School of Chemistry and Chemical Engineering, Beifang University for Nationalities, Yinchuan 750021, Ningxia, PR China; Key Opening Laboratory of Chemical Engineering (Beifang University for Nationalities), State Ethnic Affairs Commission, Yinchuan 750021, Ningxia, PR China. Electronic address: youngkincn@hotmail.com.
authors: Jin
authors: J
authors: Yang
authors: Traditional Chinese Medicine Hospital of Xinjiang, Urumqi 830000, Xinjiang, PR China.
authors: Liyan
authors: L
authors: Zhang
authors: School of Chemistry and Chemical Engineering, Beifang University for Nationalities, Yinchuan 750021, Ningxia, PR China; Key Opening Laboratory of Chemical Engineering (Beifang University for Nationalities), State Ethnic Affairs Commission, Yinchuan 750021, Ningxia, PR China.
authors: Guihua
authors: G
authors: Zhu
authors: School of Chemistry and Chemical Engineering, Beifang University for Nationalities, Yinchuan 750021, Ningxia, PR China.
authors: Li
authors: L
authors: Li
mesh_terms: D000327:Adsorption; D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D016062:Porosity; D011807:Quinolizines / Q000737:chemistry / Q000302:isolation & purification*; D012117:Resins, Synthetic / Q000737:chemistry; D029909:Sophora / Q000737:chemistry*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D010936:Plant Extracts; D011807:Quinolizines; D012117:Resins, Synthetic; C037573:oxymatrine; D000093842:Matrines
keywords: Macroporous resin; Matrine; Oxymatrine; Separation and enrichment; Sophora alopecuroides; Sophoridine