title: Metabolic engineering of Phaeodactylum tricornutum for the enhanced accumulation of omega-3 long chain polyunsaturated fatty acids.
abstract: We have engineered the diatom Phaeodactylum tricornutum to accumulate the high value omega-3 long chain polyunsaturated fatty acid docosahexaenoic acid (DHA). This was achieved by the generation of transgenic strains in which the Δ5-elongase from the picoalga Ostreococcus tauri was expressed to augment the endogenous fatty acid biosynthetic pathway. Expression of the heterologous elongase resulted in an eight-fold increase in docosahexaenoic acid content, representing a marked and valuable change in the fatty acid profile of this microalga. Importantly, DHA was shown to accumulate in triacylglycerols, with several novel triacylglycerol species being detected in the transgenic strains. In a second iteration, co-expression of an acyl-CoA-dependent Δ6-desaturase with the Δ5-elongase further increased DHA levels. Together, this demonstrates for the first time the potential of using iterative metabolic engineering to optimise omega-3 content in algae.
authors: Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, AL5 2JQ, UK.
authors: Mary L
authors: ML
authors: Hamilton
authors: Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, AL5 2JQ, UK.
authors: Richard P
authors: RP
authors: Haslam
authors: Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, AL5 2JQ, UK. Electronic address: johnathan.napier@rothamsted.ac.uk.
authors: Johnathan A
authors: JA
authors: Napier
authors: Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, AL5 2JQ, UK.
authors: Olga
authors: O
authors: Sayanova
mesh_terms: D000460:Chlorophyta / Q000201:enzymology / Q000235:genetics; D017377:Diatoms / Q000235:genetics* / Q000378:metabolism*; D004281:Docosahexaenoic Acids / Q000096:biosynthesis* / Q000235:genetics*; D044943:Fatty Acid Desaturases / Q000096:biosynthesis / Q000235:genetics; D060847:Metabolic Engineering / Q000379:methods*; D010751:Phosphopyruvate Hydratase / Q000096:biosynthesis / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D010940:Plant Proteins; D004281:Docosahexaenoic Acids; D044943:Fatty Acid Desaturases; D010751:Phosphopyruvate Hydratase
keywords: Diatoms; Genetic engineering; Lipids; Microalgae; Omega-3 long chain polyunsaturated fatty acids