title: Full-length cloning and phylogenetic analyses of translationally controlled tumour protein and ferritin genes from the Indian white prawn, Fenneropenaeus indicus (H. Milne Edwards).
abstract: Elucidation, through molecular analyses, of bacterial afflictions in commercially important aquaculture-reared shrimps is pivotal for the prevention and/or control of disease outbreaks. In this study, we examined the phylogenetic relatedness and compared the possible immune-related functional roles of both translationally controlled tumour protein (TCTP) and ferritin genes with previous studies. Both TCTP and ferritin genes were substantially upregulated in the Indian white prawn, Fenneropenaeus indicus (H. Milne Edwards), post-larvae following bath challenge with the virulent strain of bacteria, Vibrio harveyi D3. Full-length cloning of these genes by rapid amplification of complementary DNA ends -polymerase chain reaction (RACE-PCR) yielded 727-base pair (bp)-long TCTP and 1212-bp-long ferritin gene sequences. Their open reading frames (ORFs) were 507 and 510 bp, respectively. The TCTP-ORF coded for 168 amino acids with three substitutions at positions 37, 141, 155, and the ferritin ORF coded for 170 amino acids with no species-specific substitutions. Phylogenetic analysis suggested the closest relatedness of both TCTP and ferritin from F. indicus to Chinese white prawn, Fenneropenaeus chinensis (Osbeck). In addition to reporting the full-length sequences of these immune-relevant genes, this study highlighted their conserved natures, which perhaps make them important defence-related proteins in the innate immune system of F. indicus.
authors: Biological Oceanography Division, National Institute of Oceanography, Council of Scientific and Industrial Research, Goa, India.
authors: S
authors: S
authors: Nayak
authors: N
authors: N
authors: Ramaiah
authors: R M
authors: RM
authors: Meena
authors: R A
authors: RA
authors: Sreepada
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics*; D003001:Cloning, Molecular; D005293:Ferritins / Q000235:genetics*; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000145:classification* / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D010802:Phylogeny; D016415:Sequence Alignment; D000090324:Tumor Protein, Translationally-Controlled 1; D014733:Vibrio / Q000276:immunology
chemical_list: D060829:Arthropod Proteins; D014408:Biomarkers, Tumor; D000090324:Tumor Protein, Translationally-Controlled 1; D005293:Ferritins
keywords: Fenneropenaeus indicus; Vibrio harveyi; ferritin; gene expression; rapid amplification of complementary DNA ends-polymerase chain reaction; translationally controlled tumour protein