title: Plasma C-terminal proEndothelin-1 (CTproET-1) is affected by age, renal function, left atrial size and diastolic blood pressure in healthy subjects.
abstract: Endothelin-1 (ET-1) is a short chained peptide primarily of endothelial origin. Concentrations of this peptide are increased in subjects with hypertension, primary pulmonary hypertension and myocardial infarction, however its short half-life makes quantification difficult. The C-terminal of proET-1 (CTproET-1) is stoichiometrically secreted with its bioactive peptide and would be a valid method of measuring the active peptide as it has a stable half-life and is less resistant to proteolytic cleavage. The objective of this study was to understand the factors (clinical, echocardiographic and biochemical) that specifically influence plasma CTproET-1 in healthy subjects. 518 healthy volunteers were recruited from a screening study. Plasma CTproET-1 concentrations were quantified using a novel immunoluminometric sandwich assay. In multivariate analyses, age (P<0.001), diastolic BP (P=0.007), LA size (P=0.001) and eGFR (P<0.001) were independently predictive of plasma CTproET-1 levels in the healthy subjects. Therefore the interpretation of plasma CTproET-1 levels in such individuals should take into account these variables to avoid potential confounding.
authors: Department of Cardiovascular Sciences and NIHR Leicester Cardiovascular Biomedical Research Unit, Glenfield Hospital, Leicester LE3 9QP, United Kingdom. Electronic address: sanjay1bhandari@hotmail.com.
authors: S S
authors: SS
authors: Bhandari
authors: Department of Cardiovascular Sciences and NIHR Leicester Cardiovascular Biomedical Research Unit, Glenfield Hospital, Leicester LE3 9QP, United Kingdom.
authors: J E
authors: JE
authors: Davies
authors: AdrenoMed AG, Hennigsdorf, Germany.
authors: J
authors: J
authors: Struck
authors: Department of Cardiovascular Sciences and NIHR Leicester Cardiovascular Biomedical Research Unit, Glenfield Hospital, Leicester LE3 9QP, United Kingdom.
authors: L L
authors: LL
authors: Ng
mesh_terms: D000368:Aged; D000375:Aging / Q000097:blood*; D001794:Blood Pressure / Q000502:physiology*; D019332:Endothelin-1 / Q000097:blood*; D005260:Female; D006325:Heart Atria / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009929:Organ Size / Q000502:physiology; D010446:Peptide Fragments / Q000097:blood*
chemical_list: C557077:C-terminal proendothelin-1; D019332:Endothelin-1; D010446:Peptide Fragments
keywords: Age; C-terminal proEndothelin-1; Diastolic blood pressure; Endothelin-1; Healthy population; Renal disease