title: Generation of multipotent foregut stem cells from human pluripotent stem cells.
abstract: Human pluripotent stem cells (hPSCs) could provide an infinite source of clinically relevant cells with potential applications in regenerative medicine. However, hPSC lines vary in their capacity to generate specialized cells, and the development of universal protocols for the production of tissue-specific cells remains a major challenge. Here, we have addressed this limitation for the endodermal lineage by developing a defined culture system to expand and differentiate human foregut stem cells (hFSCs) derived from hPSCs. hFSCs can self-renew while maintaining their capacity to differentiate into pancreatic and hepatic cells. Furthermore, near-homogenous populations of hFSCs can be obtained from hPSC lines which are normally refractory to endodermal differentiation. Therefore, hFSCs provide a unique approach to bypass variability between pluripotent lines in order to obtain a sustainable source of multipotent endoderm stem cells for basic studies and to produce a diversity of endodermal derivatives with a clinical value.
authors: Wellcome Trust-Medical Research Council Stem Cell Institute, Anne McLaren Laboratory for Regenerative Medicine, Department of Surgery, West Forvie Site, Robinson Way, University of Cambridge, Cambridge CB2 0SZ, UK.
authors: Nicholas R F
authors: NR
authors: Hannan
authors: Robert P
authors: RP
authors: Fordham
authors: Yasir A
authors: YA
authors: Syed
authors: Victoria
authors: V
authors: Moignard
authors: Andrew
authors: A
authors: Berry
authors: Ruben
authors: R
authors: Bautista
authors: Neil A
authors: NA
authors: Hanley
authors: Kim B
authors: KB
authors: Jensen
authors: Ludovic
authors: L
authors: Vallier
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002460:Cell Line; D005775:Gastrula / Q000166:cytology*; D006801:Humans; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D015415:Biomarkers