title: Reversal effect of BM-cyclin 1 on multidrug resistance by down-regulating MRP2 in BALB/C nude mice bearing C-A120 cells.
abstract: Our previous study demonstrated that BM-cyclin 1, a traditional anti-mycoplasma drug, could effectively reverse the multidrug resistance (MDR) of C-A120 cells. The present study aims to explore the reversal effect of BM-cyclin 1 on MDR and its mechanisms in BALB/C nude mice bearing C-A120 cells. Immunoblotting analysis and reverse transcription-polymerase chain reaction (RT-PCR) were used to study the change in multidrug resistance-associated protein 2 (MRP2) induced by BM-cyclin 1. We found that the expression levels of MRP2 protein and mRNA in C-A120 cells treated with BM-cyclin 1 were reduced significantly. Chemical colorimetry revealed no significant change in the level of glutathione (GSH). In the xenograft model, the inhibitory rate of C-A120 cells growth in BM-cyclin 1 plus adriamycin (ADM) group was 52%, which was significantly higher than in control group (P<0.01). The immunoblotting and RT-PCR results conclusively demonstrated that BM-cycin 1 could significantly reduce the expression of MRP2 in transplanted tumor. In conclusion, BM-cyclin 1 could effectively reverse the MDR of C-A120 cells in vivo by suppressing the expression of MRP2.
authors: Department of Oncology, the Fourth Affiliated Hospital of Medical College, Jinan University, Guangzhou Red Cross Hospital, Guangzhou, 510220, China.
authors: Lin
authors: L
authors: Wang
authors: Guangdong Provincial Key Laboratory of Liver Disease Research, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China.
authors: Xiaoyun
authors: X
authors: Li
authors: School of Public Health, Medical College, Wuhan University of Science and Technology, Wuhan, 430065, China.
authors: Gaofeng
authors: G
authors: Jiang
authors: Department of Oncology, the Fourth Affiliated Hospital of Medical College, Jinan University, Guangzhou Red Cross Hospital, Guangzhou, 510220, China.
authors: Jizhen
authors: J
authors: Liang
authors: Department of Gastroenterology, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.
authors: Yan
authors: Y
authors: Sun
authors: Guangdong Provincial Key Laboratory of Liver Disease Research, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China. lwei6@mail.sysu.edu.cn.
authors: Wei
authors: W
authors: Liu
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000494:pharmacology; D015536:Down-Regulation; D004317:Doxorubicin / Q000494:pharmacology; D018432:Drug Resistance, Multiple / Q000187:drug effects*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008911:Minocycline / Q000494:pharmacology; D000089762:Multidrug Resistance-Associated Protein 2; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
chemical_list: D000981:Antiprotozoal Agents; D004224:Diterpenes; D004317:Doxorubicin; D008911:Minocycline; D000089762:Multidrug Resistance-Associated Protein 2; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D012333:RNA, Messenger; C000715087:ABCC2 protein, human; C074450:BM-cyclin