title: The role of electrical fields, ions, and the cortex in the morphogenesis of Acetabularia.
abstract: Electrophysiological and biochemical aspects of polarity determination and morphogenesis were studied in regenerating Acetabularia. The Ca(++), Mg(++) ionophore, A23187, reversibly inhibits the formation of apical structures (whorls and caps) but does not arrest longitudinal growth. This normal growth correlates with normal electrophysiology as reflected in an apico-basal electrical potential gradient and spontaneous recurrent action potentials which propagate from apex to base. However, the ionophore markedly elevates (32)PO 3 (3-) incorporation into the cortical cytoplasm which is normally low apically and rises to a maximum at the base. A molecular model of membrane-dependent morphogenesis is suggested.
authors: School of Biological Sciences, University of Sussex, BN1 9QG, Brighton, U.K..
authors: B C
authors: BC
authors: Goodwin
authors: S
authors: S
authors: Pateromichelakis