title: The effect of timing of gamma-irradiation on hemolysis and potassium release in leukoreduced red cell concentrates stored in SAGM.
abstract: While irradiation of red cell concentrates (RCC) prevents graft-versus-host disease in susceptible transfusion recipients, it also damages red blood cells (RBC). To understand the ability of irradiation regulations to prevent transfusion of inferior units, we irradiated 980 RCC in saline-adenine-glucose-mannitol (SAGM) using various combinations of pre-irradiation age and post-irradiation storage times, and measured hemolysis and extracellular potassium levels. We observed unacceptably high hemolysis (>0·8%) in some RCC and elevated extracellular potassium levels in all gamma-irradiated RCC. This suggests that more restrictive storage times should be considered for RCC in SAGM.
authors: Canadian Blood Services, Centre for Innovation, Vancouver, BC, Canada; Centre for Blood Research, University of British Columbia, Vancouver, BC, Canada; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.
authors: K
authors: K
authors: Serrano
authors: D
authors: D
authors: Chen
authors: A L
authors: AL
authors: Hansen
authors: E
authors: E
authors: Levin
authors: T R
authors: TR
authors: Turner
authors: J D R
authors: JD
authors: Kurach
authors: J P
authors: JP
authors: Acker
authors: D V
authors: DV
authors: Devine
mesh_terms: D000225:Adenine / Q000737:chemistry; D058731:Blood Safety; D001803:Blood Transfusion; D004912:Erythrocytes / Q000528:radiation effects*; D005720:Gamma Rays; D005947:Glucose / Q000737:chemistry; D006086:Graft vs Host Disease / Q000517:prevention & control; D006461:Hemolysis / Q000528:radiation effects*; D006801:Humans; D008353:Mannitol / Q000737:chemistry; D011188:Potassium / Q000097:blood*; D012965:Sodium Chloride / Q000737:chemistry; D012996:Solutions; D013997:Time Factors
chemical_list: D012996:Solutions; D008353:Mannitol; D012965:Sodium Chloride; D005947:Glucose; D000225:Adenine; D011188:Potassium
keywords: blood components; component production; quality control