title: Mangromicins A and B: structure and antitrypanosomal activity of two new cyclopentadecane compounds from Lechevalieria aerocolonigenes K10-0216.
abstract: Two new cyclopentadecane antibiotics, named mangromicins A and B, were separated out from the culture broth of Lechevalieria aerocolonigenes K10-0216 by Diaion HP-20, silica gel and ODS column chromatography, and were finally purified by HPLC. The chemical structures of the two novel compounds were elucidated by instrumental analyses, including various NMR, MS and X-ray crystallography. Mangromicins A and B consist of cyclopentadecane skeletons with a tetrahydrofuran unit and a 5,6-dihydro-4-hydroxy-2-pyrone moiety. Mangromicins A and B showed in vitro antitrypanosomal activity with IC50 values of 2.4 and 43.4 μg ml(-1), respectively. The IC50 values of both compounds were lower than those of cytotoxicity against MRC-5 human fetal lung fibroblast cells.
authors: Research Organization for Infection Control Sciences, Kitasato University, Tokyo, Japan.
authors: Takuji
authors: T
authors: Nakashima
authors: 1] Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan [2] Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.
authors: Masato
authors: M
authors: Iwatsuki
authors: Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.
authors: Junya
authors: J
authors: Ochiai
authors: Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.
authors: Yoshiyuki
authors: Y
authors: Kamiya
authors: Graduate School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
authors: Kenichiro
authors: K
authors: Nagai
authors: 1] Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan [2] Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.
authors: Atsuko
authors: A
authors: Matsumoto
authors: Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors: Aki
authors: A
authors: Ishiyama
authors: Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors: Kazuhiko
authors: K
authors: Otoguro
authors: 1] Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan [2] Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.
authors: Kazuro
authors: K
authors: Shiomi
authors: 1] Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan [2] Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.
authors: Yōko
authors: Y
authors: Takahashi
authors: Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors: Satoshi
authors: S
authors: Ōmura
mesh_terms: D000192:Actinomycetales / Q000737:chemistry*; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D018360:Crystallography, X-Ray; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D020128:Inhibitory Concentration 50; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018942:Macrolides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D058428:Silica Gel; D018675:Toxicity Tests; D014344:Trypanocidal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D018942:Macrolides; D014344:Trypanocidal Agents; C000588478:mangromicin A; C000588479:mangromicin B; D058428:Silica Gel