title: [Comparison of the targeting properties of 2-deoxy-D-glucose-conjugated nanoparticles to breast cancer MDA-MB-231 cells and breast fibroblasts cells].
abstract: OBJECTIVE
  To compare the differences in uptake of 2-deoxy-D-glucose (2-DG)-conjugated nanoparticles between breast carcinoma MDA-MB-231 cells with high metabolism and breast fibroblasts with normal metabolism, and investigate the feasibility of using the coated nanoparticles as a MRI-targeted contrast agent for highly metabolic carcinoma cells.
  
  
  METHODS
  The γ-Fe2O3@DMSA-DG was prepared. The glucose metabolism level of both cell lines was determined. The targeting efficacy of γ-Fe2O3@DMSA-DG and γ-Fe2O3@DMSA NPs to breast carcinoma MDA-MB-231 cells and breast fibroblasts at 10 min, 30 min, 1 h and 2 h was measured with Prussian blue staining and UV colorimetric assay. MRI was performed to visualize the changes of T2WI signal intensity.
  
  
  RESULTS
  Prussian blue staining showed more intracellular blue granules in the MDA-MB-231 cells of γ-Fe2O3@DMSA-DG NPs group than that in the γ-Fe2O3@DMSA NPs group, and the γ-Fe2O3@DMSA-DG uptake was greatly competed by free D-glucose. As revealed by UV colorimetric assay, MDA-MB-231 cells also showed that the cellular iron amount of γ-Fe2O3@DMSA-DG group was significantly higher than that of the γ-Fe2O3@DMSA group and γ-Fe2O3@DMSA-DG + D-glucose group, statistically with a significant difference between them. MRI showed that the signal intensity of γ-Fe2O3@DMSA-DG group was decrease significantly, the T2 signal intensity was decreased by 10.5%, 37.5%, 72.9%, 92.0% for 10 min, 30 min, 1 h and 2 h, respectively. In contrast, the signal intensity did not show obvious decrease in the γ-Fe2O3@DMSA-DG group, the T2 signal intensity was decreased by 8.5%, 11.4%, 32.0%, 76.7% for 10 min, 30 min, 1 h and 2 h, respectively. However, HUM-CELL-0056 cells did not produce apparent difference for positive staining in the γ-Fe2O3@DMSA-DG group, γ-Fe2O3@DMSA group and γ-Fe2O3@DMSA-DG+D-glucose group, and the signal intensity also did not produce apparent difference.
  
  
  CONCLUSIONS
  γ-Fe2O3@DMSA-DG has good targeting ability to highly metabolic breast carcinoma (MDA-MB-231) cells. It is feasible to serve as a specific MRI-targeted contrast agent for highly metabolic carcinoma cells, and deserves further studies in vivo.
authors: Department of Radiology, the Affiliated Renmin Hospital of Jiangsu University, Zhenjiang 212002, China.
authors: Peng
authors: P
authors: Wang
authors: Xiu-hong
authors: XH
authors: Shan
authors: Fei
authors: F
authors: Xiong
authors: Ning
authors: N
authors: Gu
authors: Hui
authors: H
authors: Qian
authors: Yu
authors: Y
authors: Fan
authors: Ya-fei
authors: YF
authors: Wang
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D003124:Colorimetry / Q000379:methods; D003287:Contrast Media / Q000493:pharmacokinetics; D003847:Deoxyglucose / Q000737:chemistry / Q000493:pharmacokinetics*; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry / Q000493:pharmacokinetics*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007501:Iron / Q000378:metabolism; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058726:Nanoconjugates / Q000737:chemistry; D010316:Particle Size; D004113:Succimer / Q000737:chemistry / Q000493:pharmacokinetics*
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; D058726:Nanoconjugates; C000499:ferric oxide; D003847:Deoxyglucose; D004113:Succimer; D007501:Iron; D005947:Glucose