title: Engineering BmpA as a carrier for surface display of IgG-binding domain on Lactococcus lactis.
abstract: Basic membrane protein A (BmpA) is a potential carrier protein for surface display of the IgG-binding domain on Lactococcus lactis. We have shown that it can increase the adhesion of bacteria to the intestinal cell model by 1.3-fold and have improved BmpA-based surface display by engineering the BmpA molecule. The bulk of the BmpA molecule was shown to be important in surface display; however, limited shortening (variant Bmp1) resulted in a large increase in the surface display ability. The closeness of the N- and the C-terminals in the Bmp1 model and the inefficiency of the spacer suggest that the distance of the passenger from the membrane is not of prime importance in surface display.
authors: Department of Biotechnology, JoŽef Stefan Institute, Jamova 39, SI-1000 Ljubljana, Slovenia.
authors: Petra
authors: P
authors: Zadravec
authors: Anja
authors: A
authors: Mavrič
authors: Bojana
authors: B
authors: Bogovič Matijasic
authors: Borut
authors: B
authors: Štrukelj
authors: Aleš
authors: A
authors: Berlec
mesh_terms: D001422:Bacterial Adhesion / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018938:Caco-2 Cells; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D061505:Cell Surface Display Techniques; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D013294:Lactococcus lactis / Q000235:genetics*; D008074:Lipoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D009154:Mutation; D015202:Protein Engineering; D055550:Protein Stability; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D008074:Lipoproteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins
keywords: Lactococcus lactis; basic membrane protein A; carrier protein; surface display