title: Inhibitory effect of valsartan on the intestinal absorption and renal excretion of bestatin in rats.
abstract: Peptidomimetic drugs have favorable bioavailability owing to H(+)/peptide transporter 1 (PEPT1) located in the intestine. Sartans are commonly used and likely coadministered with peptidomimetic drugs in the clinic; however, in vivo interactions between sartans and peptidomimetic drugs have not been systemically understood. Herein, the effect and mechanism of sartans on the intestinal absorption and renal excretion of the dipeptide-like drug bestatin were investigated. Following oral combination with valsartan, the plasma concentration and area under the plasma concentration-time curve of bestatin in rats decreased significantly. Bestatin absorption in rat-everted intestinal sacs was dramatically reduced by valsartan. Sartans exhibited concentration-dependent inhibition on the uptake of bestatin in human PEPT1 (hPEPT1)-HeLa cells. The cumulative urinary excretion and renal clearance of the two drugs in rats decreased after intravenous coadministration. Moreover, decreased uptake of the two drugs was observed in rats' kidney slices and human organic anion transporter (hOAT)1/hOAT3-transfected cells when coadministered. The results suggest that the intestinal absorption and renal excretion of bestatin in rats were inhibited by coadministered valsartan. Interestingly, the half-maximal inhibitory concentration (IC50) values of valsartan for PEPT1 and OAT1/3 were comparable to the theoretically estimated local drug concentration and the clinical unbound concentration, respectively, proposing possible drug-drug interaction in humans via PEPT1 and OAT1/3, which should be paid particular attention when bestatin and valsartan are coadministrated clinically.
authors: Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Dalian, Liaoning, People's Republic of China.
authors: Xiaokui
authors: X
authors: Huo
authors: Qi
authors: Q
authors: Liu
authors: Changyuan
authors: C
authors: Wang
authors: Qiang
authors: Q
authors: Meng
authors: Huijun
authors: H
authors: Sun
authors: Jinyong
authors: J
authors: Peng
authors: Xiaochi
authors: X
authors: Ma
authors: Pengyuan
authors: P
authors: Sun
authors: Kexin
authors: K
authors: Liu
mesh_terms: D061605:Administration, Intravenous; D000284:Administration, Oral; D000465:Algorithms; D047228:Angiotensin II Type 1 Receptor Blockers / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics* / Q000652:urine; D019540:Area Under Curve; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004347:Drug Interactions; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D007408:Intestinal Absorption / Q000187:drug effects*; D007930:Leucine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000652:urine; D008297:Male; D027362:Organic Anion Transport Protein 1 / Q000378:metabolism; D029362:Organic Anion Transporters, Sodium-Independent / Q000378:metabolism; D000074011:Peptide Transporter 1; D051381:Rats; D017208:Rats, Wistar; D027981:Symporters / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D013777:Tetrazoles / Q000494:pharmacology*; D014633:Valine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000068756:Valsartan
chemical_list: D047228:Angiotensin II Type 1 Receptor Blockers; D000900:Anti-Bacterial Agents; D027362:Organic Anion Transport Protein 1; D029362:Organic Anion Transporters, Sodium-Independent; D000074011:Peptide Transporter 1; C489940:Slc15a1 protein, rat; C421043:Slc22a6 protein, rat; D027981:Symporters; D013777:Tetrazoles; C114338:organic anion transport protein 3; D000068756:Valsartan; D007930:Leucine; D014633:Valine; C012211:ubenimex
keywords: drug interaction; intestinal absorption; organic anion transporters; peptide transporters; pharamcokinetics; renal excretion