title: Validation of a spectrophotometric assay method for bisoprolol using picric acid.
abstract: Bisoprolol is a drug belonging to beta blockers drugs used primarily for the treatment of cardiovascular diseases.
  
  
  OBJECTIVE
  A spectrophotometric method for quantitative determination of bisoprolol was developed based on the formation of a complex combination between bisoprolol and picric acid.
  
  
  METHODS
  The complex combination of bisoprolol and picric acid has a maximum absorbance peak at 420 nm. Optimum working conditions were established and the method was validated.
  
  
  RESULTS
  The method presented a good linearity in the concentration range 5-120 microg/ml (regression coefficient r2 = 0.9992). The RSD for the precision of the method was 1.74 and for the intermediate precision 1.43, and recovery values ranged between 98.25-101.48%.
  
  
  CONCLUSIONS
  The proposed and validated spectrophotometric method for the determination of bisoprolol is simple and cost effective.
authors: Analytical Chemistry Department, Faculty of Pharmacy, University of Medicine and Pharmacy "Grigore T. Popa" - Iaşi.
authors: Alina-Diana
authors: AD
authors: Panainte
authors: Nela
authors: N
authors: Bibire
authors: Gladiola
authors: G
authors: Tântaru
authors: M
authors: M
authors: Apostu
authors: Mădălina
authors: M
authors: Vieriu
mesh_terms: D058671:Adrenergic beta-1 Receptor Antagonists / Q000032:analysis* / Q000737:chemistry; D017298:Bisoprolol / Q000032:analysis* / Q000737:chemistry; D007202:Indicators and Reagents; D010851:Picrates; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods*
chemical_list: D058671:Adrenergic beta-1 Receptor Antagonists; D007202:Indicators and Reagents; D010851:Picrates; C005858:picric acid; D017298:Bisoprolol