title: Ara h 6 complements Ara h 2 as an important marker for IgE reactivity to peanut.
abstract: The similarities of two major peanut allergens, Ara h 2 and Ara h 6, in molecular size, amino acid sequence, and structure have made it difficult to obtain natural Ara h 6 free of Ara h 2. The objectives of this study were to purify natural Ara h 6 that is essentially free of Ara h 2 and to compare its IgE reactivity and potency in histamine release assays to Ara h 2. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the highly purified allergen (<0.01% Ara h 2) revealed a single 14.5 kD band, and the identity of Ara h 6 was confirmed by liquid chromatography-tandem mass spectrometry. Ara h 6 showed a higher seroprevalence in chimeric IgE enzyme-linked immunosorbent assay (n = 54) but a weaker biological activity in basophil histamine release assays than Ara h 2. Purified Ara h 6 will be useful for diagnostic IgE antibody assays as well as molecular and cellular studies to investigate the immunological mechanisms of peanut allergy.
authors: INDOOR Biotechnologies, Incorporated , 1216 Harris Street, Charlottesville, Virginia 22903, United States.
authors: Audrey E
authors: AE
authors: Koid
authors: Martin D
authors: MD
authors: Chapman
authors: Robert G
authors: RG
authors: Hamilton
authors: Ronald
authors: R
authors: van Ree
authors: Serge A
authors: SA
authors: Versteeg
authors: Stephen C
authors: SC
authors: Dreskin
authors: Stef J
authors: SJ
authors: Koppelman
authors: Sabina
authors: S
authors: Wünschmann
mesh_terms: D055516:2S Albumins, Plant / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D052179:Antigens, Plant / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D010367:Arachis / Q000737:chemistry* / Q000276:immunology; D001491:Basophils / Q000276:immunology; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D006636:Histamine Release; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008969:Molecular Sequence Data; D021183:Peanut Hypersensitivity / Q000276:immunology*; D016415:Sequence Alignment
chemical_list: D055516:2S Albumins, Plant; D052179:Antigens, Plant; C500890:Ara h 2 allergen, Arachis hypogaea; C492737:Ara h 6 allergen, Arachis hypogaea; D006023:Glycoproteins; D007073:Immunoglobulin E
keywords: IgE reactivity; allergen; histamine release; peanut