title: Long-term self-renewal of human ES/iPS-derived hepatoblast-like cells on human laminin 111-coated dishes.
abstract: The establishment of self-renewing hepatoblast-like cells (HBCs) from human pluripotent stem cells (PSCs) would realize a stable supply of hepatocyte-like cells for medical applications. However, the functional characterization of human PSC-derived HBCs was not enough. To purify and expand human PSC-derived HBCs, human PSC-derived HBCs were cultured on dishes coated with various types of human recombinant laminins (LN). Human PSC-derived HBCs attached to human laminin-111 (LN111)-coated dish via integrin alpha 6 and beta 1 and were purified and expanded by culturing on the LN111-coated dish, but not by culturing on dishes coated with other laminin isoforms. By culturing on the LN111-coated dish, human PSC-derived HBCs were maintained for more than 3 months and had the ability to differentiate into both hepatocyte-like cells and cholangiocyte-like cells. These expandable human PSC-derived HBCs would be manageable tools for drug screening, experimental platforms to elucidate mechanisms of hepatoblasts, and cell sources for hepatic regenerative therapy.
authors: Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871, Japan ; Laboratory of Hepatocyte Differentiation, National Institute of Biomedical Innovation, Osaka 567-0085, Japan ; iPS Cell-Based Research Project on Hepatic Toxicity and Metabolism, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871, Japan.
authors: Kazuo
authors: K
authors: Takayama
authors: Yasuhito
authors: Y
authors: Nagamoto
authors: Natsumi
authors: N
authors: Mimura
authors: Katsuhisa
authors: K
authors: Tashiro
authors: Fuminori
authors: F
authors: Sakurai
authors: Masashi
authors: M
authors: Tachibana
authors: Takao
authors: T
authors: Hayakawa
authors: Kenji
authors: K
authors: Kawabata
authors: Hiroyuki
authors: H
authors: Mizuguchi
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D017690:Cell Transplantation; D022781:Hepatocytes / Q000166:cytology / Q000637:transplantation; D006801:Humans; D039503:Integrin alpha6 / Q000378:metabolism; D019012:Integrin beta1 / Q000378:metabolism; D007797:Laminin / Q000494:pharmacology; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology*
chemical_list: D039503:Integrin alpha6; D019012:Integrin beta1; D007797:Laminin