title: Prime-boost vaccination with plasmid DNA followed by recombinant vaccinia virus expressing BgGARP induced a partial protective immunity to inhibit Babesia gibsoni proliferation in dogs.
abstract: A heterologous prime-boost vaccination regime with DNA and recombinant vaccinia virus (rvv) vectors expressing relevant antigens has been shown to induce effective immune responses against several infectious pathogens. In this study, we describe the effectiveness of the prime-boost strategy by immunizing dogs with a recombinant plasmid followed by vaccinia virus, both of which expressed the glutamic acid-rich protein (BgGARP) of Babesia gibsoni. The dogs immunized with the prime-boost regime developed a significantly high level of specific antibodies against BgGARP when compared with the control groups. The antibody level was strongly increased after a booster immunization with a recombinant vaccinia virus. Two weeks after the booster immunization with a recombinant vaccinia virus expressing BgGARP, the dogs were challenged with B. gibsoni parasite. The dogs immunized with the prime-boost regime showed partial protection, manifested as a significantly low level of parasitemia. These results indicated that this type of DNA/rvv prime-boost immunization approach may have use against B. gibsoni infection in dogs.
authors: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, 080-8555, Japan.
authors: Shinuo
authors: S
authors: Cao
authors: Ahmed Abdelmoniem
authors: AA
authors: Mousa
authors: Gabriel Oluga
authors: GO
authors: Aboge
authors: Ketsarin
authors: K
authors: Kamyingkird
authors: Mo
authors: M
authors: Zhou
authors: Paul Franck Adjou
authors: PF
authors: Moumouni
authors: Mohamad Alaa
authors: MA
authors: Terkawi
authors: Tatsunori
authors: T
authors: Masatani
authors: Yoshifumi
authors: Y
authors: Nishikawa
authors: Hiroshi
authors: H
authors: Suzuki
authors: Shinya
authors: S
authors: Fukumoto
authors: Xuenan
authors: X
authors: Xuan
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001403:Babesia / Q000235:genetics / Q000276:immunology*; D001404:Babesiosis / Q000276:immunology / Q000517:prevention & control*; D004283:Dog Diseases / Q000276:immunology / Q000517:prevention & control*; D004285:Dogs; D004337:Drug Carriers; D005260:Female; D018512:Parasitemia / Q000517:prevention & control; D016052:Protozoan Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D016896:Treatment Outcome; D014611:Vaccination / Q000379:methods*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics
chemical_list: D000913:Antibodies, Protozoan; D004337:Drug Carriers; D016052:Protozoan Vaccines; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic