title: Orsay, Santeuil and Le Blanc viruses primarily infect intestinal cells in Caenorhabditis nematodes.
abstract: The discoveries of Orsay, Santeuil and Le Blanc viruses, three viruses infecting either Caenorhabditis elegans or its relative Caenorhabditis briggsae, enable the study of virus-host interactions using natural pathogens of these two well-established model organisms. We characterized the tissue tropism of infection in Caenorhabditis nematodes by these viruses. Using immunofluorescence assays targeting proteins from each of the viruses, and in situ hybridization, we demonstrate viral proteins and RNAs localize to intestinal cells in larval stage Caenorhabditis nematodes. Viral proteins were detected in one to six of the 20 intestinal cells present in Caenorhabditis nematodes. In Orsay virus-infected C. elegans, viral proteins were detected as early as 6h post-infection. The RNA-dependent RNA polymerase and capsid proteins of Orsay virus exhibited different subcellular localization patterns. Collectively, these observations provide the first experimental insights into viral protein expression in any nematode host, and broaden our understanding of viral infection in Caenorhabditis nematodes.
authors: Departments of Molecular Microbiology and Pathology and Immunology, Washington University in St. Louis School of Medicine, 660 S. Euclid Avenue, St. Louis, MO, USA.
authors: Carl J
authors: CJ
authors: Franz
authors: Hilary
authors: H
authors: Renshaw
authors: Lise
authors: L
authors: Frezal
authors: Yanfang
authors: Y
authors: Jiang
authors: Marie-Anne
authors: MA
authors: Félix
authors: David
authors: D
authors: Wang
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000821:virology*; D007422:Intestines / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D056189:Viral Tropism; D018406:Virus Physiological Phenomena; D014780:Viruses / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*
chemical_list: D014764:Viral Proteins
keywords: Immunofluorescence; Nematode virology; Tissue tropism