title: Characterization of developing oat seed mRNA: evidence for many globulin mRNAs.
abstract: Polyadenylated mRNA from developing oat (Avena sativa L.) seeds was isolated and analyzed. Prominent mRNA species of 18S, 15S and 12S were observed; the 18S mRNA was judged to be esentially free of ribosomal RNA by hybridization analysis. Size fractionation andin vitro translation of this mRNA was performed. SDS, IEF-SDS gel electrophoresis and immunoprecipitation were used to analyze the translation products. It is shown that globulin mRNA (18S) accounts for roughly 30% of the total mRNA in developing seeds, the 12S and 15S mRNAs accounting for the remainder. The 18S mRNA directs the synthesis of a series of distinct but related polypeptides, suggesting that some of the heterogeneity seen in the oat globulins is at the amino acid sequence level.
authors: Department of Biochemistry, School of Medicine, University of Ottawa, K1N9B4, Ottawa, Ontario, Canada.
authors: S
authors: S
authors: Fabijanski
authors: G J
authors: GJ
authors: Matlashewski
authors: I
authors: I
authors: Altosaar