title: I-DNASE21 system: development and SWGDAM validation of a new STR 21-plex reaction.
abstract: I-DNASE21 is a new STR multiplex system that amplifies 21 STR autosomal loci, plus the amelogenin locus in one reaction. This system has been designed to analyze all the STR loci included in the Combined DNA Index System (CODIS), Interpol Standard Set of Loci (ISSL), Extended European Standard Set (ESS-Extended), UK National Criminal Intelligence DNA Database (NDNAD) and German Core loci (GCL). This manuscript presents the validation of the I-DNASE21 system according to the revised guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM). The results of this validation, added to the extremely high discriminatory power showed, suggest that I-DNASE21 could be a potentially helpful tool for identification and kinship determination even in complex paternity cases.
authors: BIOMICs Research Group, Centro de Investigación "Lascaray" Ikergunea, Universidad del País Vasco UPV/EHU, Avda. Miguel de Unamuno 3, 01006 Vitoria-Gasteiz, Spain. Electronic address: jmao1982@gmail.com.
authors: Jose María
authors: JM
authors: Aznar
authors: David
authors: D
authors: Celorrio
authors: Adrian
authors: A
authors: Odriozola
authors: Stephan
authors: S
authors: Köhnemann
authors: María Luisa
authors: ML
authors: Bravo
authors: Juan Jose
authors: JJ
authors: Builes
authors: Heidi
authors: H
authors: Pfeiffer
authors: René J
authors: RJ
authors: Herrera
authors: Marian M
authors: MM
authors: de Pancorbo
mesh_terms: D000818:Animals; D030541:Databases, Genetic; D003851:Deoxyribonucleases / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D013045:Species Specificity
chemical_list: D003851:Deoxyribonucleases
keywords: DNA database; DNA typing; Forensic; Kinship; Multiplex; STR