title: Novel association analysis between HLA-DQB1 polymorphisms and rectal cancer based on a cross-validation design.
abstract: A new study design based on cross-validation of the age at the onset of rectal cancer and the differences between the frequency distributions of relevant genes in 2 groups was developed for identification of disease-related HLA. Patients with rectal cancer were recruited and their age at the time of the first surgery was recorded. The genetic variants of HLA-DQB1 were genotyped using an HLA-DQB1 PCR-SSP typing kit. Allele frequencies were compared with control population. The mean age of patients with and without the alleles was compared. The frequency values of HLA-DQB1*02 were 12.3% higher in the cancer group than in the control population (P < 0.05). The median ages of the subjects with and without HLA-DQB1*02 were 54.0 and 61.0 years, respectively, with significant difference observed between the ages for these groups (P < 0.05). The median ages of the subjects with and without HLA-DQB1*03 were 62.0 and 58.0 years, respectively, and a significant difference was observed. The cross-validation of the 2 above mentioned analytical results showed that a statistically significant difference was noted for HLA-DQB1*02 (P < 0.05), whereas no such statistically significant difference was observed for HLA-DQB1*03. HLA-DQB1*02 allele was related to cancer susceptibility. The new analysis method may be an efficient and reliable approach for the identification of disease-related HLA.
authors: Dalian Fifth Hospital, Dalian, China.
authors: F Z
authors: FZ
authors: Tong
authors: W J
authors: WJ
authors: Yu
authors: H
authors: H
authors: Liu
mesh_terms: D017677:Age Distribution; D017668:Age of Onset; D000368:Aged; D003627:Data Interpretation, Statistical; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies / Q000379:methods*; D020022:Genetic Predisposition to Disease; D059866:HLA-DQ beta-Chains / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008957:Models, Genetic; D011110:Polymorphism, Genetic; D012004:Rectal Neoplasms / Q000453:epidemiology / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen