title: Exposure to bisphenol-A affects fear memory and histone acetylation of the hippocampus in adult mice.
abstract: Bisphenol-A (BPA), an environmental endocrine disruptor, has been reported to possess weak estrogenic, anti-estrogenic, and anti-androgen properties. Previous evidence indicates that perinatal exposure to low levels of BPA affects anxiety-like and cognitive behaviors in adult rodents. The present study aims to investigate the effect of BPA on emotional memory using the contextual fear conditioning of male mice in adulthood exposed to BPA for 90days. The results indicated that exposure to BPA increased the freezing time 1h and 24h after fear conditioning training. Furthermore, western blot analyses showed that BPA exposure decreased the level of N-methyl-d-aspartic acid (NMDA) receptor subunit NR1 and increased the expression of histone deacetylase 2 (HDAC2) before fear conditioning training in the hippocampus of male mice. One and twenty-four hours after fear conditioning training, BPA enhanced the changes of the expressions of NR1, phosphorylated extracellular regulated protein kinases (ERK1/2), and histone acetylation induced by contextual fear conditioning in the hippocampus. These results suggest that long term exposure to BPA enhanced fear memory by the concomitant increased level of NMDA receptor and/or the enhanced histone acetylation in the hippocampus, which may be associated with activation of ERK1/2 signaling pathway.
authors: Chemistry and Life Sciences College, Zhejiang Normal University, China.
authors: Qin
authors: Q
authors: Zhang
authors: Chemistry and Life Sciences College, Zhejiang Normal University, China; Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University, China. Electronic address: xuxh63@zjnu.cn.
authors: Xiaohong
authors: X
authors: Xu
authors: Chemistry and Life Sciences College, Zhejiang Normal University, China.
authors: Tao
authors: T
authors: Li
authors: Chemistry and Life Sciences College, Zhejiang Normal University, China.
authors: Yang
authors: Y
authors: Lu
authors: Chemistry and Life Sciences College, Zhejiang Normal University, China.
authors: Qin
authors: Q
authors: Ruan
authors: Kindergarten Teachers College, Zhejiang Normal University, China.
authors: Yingjun
authors: Y
authors: Lu
authors: Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University, China.
authors: Qinwen
authors: Q
authors: Wang
authors: Chemistry and Life Sciences College, Zhejiang Normal University, China.
authors: Fangni
authors: F
authors: Dong
authors: Chemistry and Life Sciences College, Zhejiang Normal University, China.
authors: Yanling
authors: Y
authors: Yang
authors: Chemistry and Life Sciences College, Zhejiang Normal University, China.
authors: Guangxia
authors: G
authors: Zhang
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001559:Benzhydryl Compounds / Q000494:pharmacology*; D003213:Conditioning, Psychological / Q000187:drug effects; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D005239:Fear / Q000187:drug effects*; D046352:Freezing Reaction, Cataleptic / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008297:Male; D008568:Memory / Q000187:drug effects*; D051379:Mice; D008813:Mice, Inbred ICR; D010636:Phenols / Q000494:pharmacology*; D010766:Phosphorylation; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004968:Estrogens, Non-Steroidal; D006657:Histones; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol-A; Extracellular regulated protein kinases (ERK1/2); Fear conditioning; Histone acetylation