title: Effect of interleukin-17A and interleukin-17F gene polymorphisms on the risk of gastric cancer in a Chinese population.
abstract: We selected six tagged single-nucleotide polymorphisms (SNPs) in the IL-17A and IL-17F genes, and evaluated the relationship between the six common SNPs and H. pylori infection, tobacco smoking and subsites of gastric cancer in gastric cancer patients. Genotyping of IL-17A (rs2275913, rs3748067 and rs3819025) and IL-17A (rs763780, rs9382084, and rs12203582) was performed in a 384-well plate format on the MassARRAY® platform. An unconditional multiple logistical regression model was performed to determine the association between IL-17A and IL-17F genetic variations and gastric cancer risk. Unconditional logistic regression analysis showed that subjects carrying the rs2275913AA and rs3748067 TT genotypes were 1.70 and 3.45 times more likely to develop gastric cancer. Furthermore, rs2275913 and rs3748067 genetic variants significantly interacted with H. pylori infection on the risk of gastric cancer. The interaction between rs3748067 and rs9382084 genetic variants and tobacco smoking trend was significant. In addition, rs2275913, rs3748067 and rs9382084 genetic variants were only associated with non-cardia gastric cancer. The findings suggest that the rs2275913, rs3748067 and rs9382084 polymorphisms increase the risk of gastric cancer, and they interact with H. pylori infection, tobacco smoking and subsites of gastric cancer. These findings could be helpful in identifying individuals at increased risk for developing gastric cancer.
authors: Department of General Surgery, Zhumadian Centre Hospital, Zhumadian, China.
authors: Zhu
authors: Z
authors: Qinghai
authors: Department of General Surgery, Zhumadian Centre Hospital, Zhumadian, China. Electronic address: zhangxiaoqiao7878@163.com.
authors: Wang
authors: W
authors: Yanying
authors: Department of Oncology, Zhumadian Centre Hospital, Zhumadian, China.
authors: Chen
authors: C
authors: Yunfang
authors: Department of General Surgery, PLA Jinan General Hospital, Jinan, China.
authors: Zhang
authors: Z
authors: Xukui
authors: Department of General Surgery, PLA Jinan General Hospital, Jinan, China.
authors: Zhang
authors: Z
authors: Xiaoqiao
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D016481:Helicobacter Infections / Q000235:genetics* / Q000382:microbiology; D016480:Helicobacter pylori / Q000472:pathogenicity; D006801:Humans; D020381:Interleukin-17 / Q000235:genetics*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012907:Smoking / Q000235:genetics; D013274:Stomach Neoplasms / Q000209:etiology / Q000235:genetics* / Q000382:microbiology
chemical_list: D020381:Interleukin-17
keywords: CIs; DNA; Gastric cancer; Genetic variants; H. pylori; H. pylori infection; Helicobacter pylori; IL-17; Interleukin-17A; Interleukin-17F; MALDI-TOF; OR; PCR; SD; SNP; Smoking; TNM stage; confidence intervals; dNTP; deoxy-ribonucleoside triphosphate; deoxyribonucleic acid; interleukin-17; matrix-assisted laser desorption/ionization time-of-flight; odds ratios; polymerase chain reaction; single-nucleotide polymorphisms; standard deviation; tumor node metastasis stage