title: The attractive recombinant phytase from Bacillus licheniformis: biochemical and molecular characterization.
abstract: The phyL gene encoding phytase from the industrial strain Bacillus licheniformis ATCC 14580 (PhyL) was cloned, sequenced, and overexpressed in Escherichia coli. Biochemical characterization demonstrated that the recombinant enzyme has an apparent molecular weight of nearly 42 kDa. Interestingly, this enzyme was optimally active at 70-75 °C and pH 6.5-7.0. This enzyme is distinguishable by the fact that it preserved more than 40 % of its activity at wide range of temperatures from 4 to 85 °C. This new phytase displayed also a high specific activity of 316 U/mg. For its maximal activity and thermostability, this biocatalyst required only 0.6 mM of Ca(2+) ion and exhibited high catalytic efficiency of 8.3 s(-1) μM(-1) towards phytic acid.
authors: Faculty of Sciences of Gafsa-Unit of Macromolecular Biochemistry and Genetic, Department of Life Sciences, Zarroug, 2112, Gafsa, Tunisia.
authors: Mohamed Ali
authors: MA
authors: Borgi
authors: Mouna
authors: M
authors: Khila
authors: Samira
authors: S
authors: Boudebbouze
authors: Nushin
authors: N
authors: Aghajari
authors: Florette
authors: F
authors: Szukala
authors: Nicolas
authors: N
authors: Pons
authors: Emmanuelle
authors: E
authors: Maguin
authors: Moez
authors: M
authors: Rhimi
mesh_terms: D010832:6-Phytase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics*; D002118:Calcium / Q000378:metabolism; D002413:Cations, Divalent / Q000378:metabolism; D003001:Cloning, Molecular; D003067:Coenzymes / Q000378:metabolism; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010833:Phytic Acid / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment; D013696:Temperature
chemical_list: D002413:Cations, Divalent; D003067:Coenzymes; D011994:Recombinant Proteins; D010833:Phytic Acid; D010832:6-Phytase; D002118:Calcium