title: Isolation and characterization of a buffalograss (Buchloe dactyloides) dehydration responsive element binding transcription factor, BdDREB2.
abstract: Dehydration responsive element binding (DREB) transcription factors play an important role in the regulation of stress-related genes. These factors contribute to resistance to different abiotic stresses. In the present study, a novel DREB transcription factor, BdDREB2, isolated from Buchloe dactyloides, was cloned and characterized. The BdDREB2 protein was estimated to have a molecular weight of 28.36kDa, a pI of 5.53 and a typical AP2/ERF domain. The expression of BdDREB2 was involved in responses to drought and salt stresses. Overexpression of BdDREB2 in tobacco showed higher relative water and proline content, and was associated with lower MDA content under drought stress, suggesting that the transgenic tobacco may tolerate drought stress better. Results demonstrate that BdDREB2 may play an important role in the regulation of abiotic stress responses, and mediate many physiological pathways that enhance stress tolerance in plants.
authors: Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: zhangpgrass@126.com.
authors: Pan
authors: P
authors: Zhang
authors: Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: yangpeizhi@126.com.
authors: Peizhi
authors: P
authors: Yang
authors: Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: zhangzq19890102@126.com.
authors: Zhiqiang
authors: Z
authors: Zhang
authors: Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: hanbo1983@126.com.
authors: Bo
authors: B
authors: Han
authors: Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: wangweidong5153@126.com.
authors: Weidong
authors: W
authors: Wang
authors: Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: wyf806346256@163.com.
authors: Yafang
authors: Y
authors: Wang
authors: Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: 970539096@qq.com.
authors: Yuman
authors: Y
authors: Cao
authors: Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: hutianming@126.com.
authors: Tianming
authors: T
authors: Hu
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics; D003001:Cloning, Molecular; D003681:Dehydration; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D006109:Poaceae / Q000235:genetics* / Q000502:physiology*; D020218:Response Elements; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013312:Stress, Physiological / Q000235:genetics; D014026:Nicotiana / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D029681:Arabidopsis Proteins; C114579:DREB2A protein, Arabidopsis; D014157:Transcription Factors
keywords: ANOVA; AP2/EREBP; AP2/ERF; APETLA2/ethylene responsive element binding factor; APETLA2/ethylene responsive element binding protein; BdDREB2; Buchloe dactyloides dehydration responsive element binding transcription factor 2; Buffalograss; CaMV 35S; DRE/CRT; DREB; Drought stress; Expression analysis; GSP; LSD; MDA; NaCl; NaClO; ORF; PEG 6000; RACE; RT-PCR; RWC; TBA; Tobacco transformation; WT; analysis of variance; cauliflower mosaic virus 35S; dehydration responsive element binding transcription factor; dehydration-responsive element/C-repeat; gene specific primers; least significant difference; malonyldialdehyde; open reading frame; polyethylene glycol 6000; rapid amplification of cDNA end; relative water content; reverse transcription-polymerase chain reaction; sodium chloride; sodium hypochlorite; thiobarbituric acid; wild type