title: Anti-inflammatory effect of amlodipine plus atorvastatin treatment on carotid atherosclerosis in zucker metabolic syndrome rats.
abstract: To investigate the effects of amlodipine in combination with atorvastatin on carotid atherosclerotic changes in metabolic syndrome, 8-week-old Zucker fatty rats were treated with vehicle, amlodipine, atorvastatin, or amlodipine in combination with atorvastatin for 28 days. Histological studies of common carotid arteries showed that lipid deposition determined by Sudan III staining was significantly reduced in rats treated with amlodipine or atorvastatin alone and was further reduced by amlodipine in combination with atorvastatin. Immunohistochemical studies of the pro-inflammatory cytokine tumor necrosis factor (TNF)-α, the arterial calcification initiator bone morphogenetic protein (BMP) 2, the angiogenic factor Notch1, and the smooth muscle cell marker α-smooth muscle actin (SMA) showed that the high expression of all four protein in vehicle-treated rats was greatly decreased by amlodipine, atorvastatin, or amlodipine in combination with atorvastatin, in ascending order. Double immunostaining showed marked colocalization of TNF-α with bone morphogenetic protein 2 and Notch1 with α-SMA in the vehicle group, which was greatly reduced by amlodipine plus atorvastatin. These data suggest that combination therapy may be more effective in preventing atherosclerotic processes and subsequent carotid vascular events than administrating amlodipine or atorvastatin alone in metabolic syndrome.
authors: Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikatacho, Okayama, 700-8558, Japan.
authors: Xuemei
authors: X
authors: Zhang
authors: Fengfeng
authors: F
authors: Tian
authors: Hiromi
authors: H
authors: Kawai
authors: Tomoko
authors: T
authors: Kurata
authors: Shoko
authors: S
authors: Deguchi
authors: Kentaro
authors: K
authors: Deguchi
authors: Jingwei
authors: J
authors: Shang
authors: Ning
authors: N
authors: Liu
authors: Wentao
authors: W
authors: Liu
authors: Yoshio
authors: Y
authors: Ikeda
authors: Tohru
authors: T
authors: Matsuura
authors: Tatsushi
authors: T
authors: Kamiya
authors: Koji
authors: K
authors: Abe