title: Quantitative analysis of herbivore-induced cytosolic calcium by using a Cameleon (YC 3.6) calcium sensor in Arabidopsis thaliana.
abstract: Ca(2+) is a key player in plant cell responses to biotic and abiotic stress. Owing to the central role of cytosolic Ca(2+) ([Ca(2+)]cyt) during early signaling and the need for precise determination of [Ca(2+)]cyt variations, we used a Cameleon YC 3.6 reporter protein expressed in Arabidopsis thaliana to quantify [Ca(2+)]cyt variations upon leaf mechanical damage (MD), herbivory by 3rd and 5th instar larvae of Spodoptera littoralis and S. littoralis oral secretions (OS) applied to MD. YC 3.6 allowed a clear distinction between MD and herbivory and discriminated between the two larvae instars. To our knowledge this is the first report of quantitative [Ca(2+)]cyt determination upon herbivory using a Cameleon calcium sensor.
authors: Plant Physiology Unit, Dept. Life Sciences and Systems Biology, University of Turin, Italy. Electronic address: francesca.verrillo@unito.it.
authors: Francesca
authors: F
authors: Verrillo
authors: Plant Physiology Unit, Dept. Life Sciences and Systems Biology, University of Turin, Italy. Electronic address: andrea.occhipinti@unito.it.
authors: Andrea
authors: A
authors: Occhipinti
authors: Research and Innovation Center Biology and Genomic of Fruit Plants, Fondazione Edmund Mach FEM San Michele all'Adige (TN), Italy. Electronic address: chidubt@gmail.com.
authors: Chidananda Nagamangala
authors: CN
authors: Kanchiswamy
authors: Plant Physiology Unit, Dept. Life Sciences and Systems Biology, University of Turin, Italy. Electronic address: massimo.maffei@unito.it.
authors: Massimo E
authors: ME
authors: Maffei
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology*; D002147:Calmodulin; D003600:Cytosol / Q000378:metabolism; D060434:Herbivory; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins
chemical_list: D002147:Calmodulin; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; C579281:YCaM3.6 protein; D002118:Calcium
keywords: Arabidopsis thaliana; Calcium signaling; Cameleon YC 3.6; Herbivory; Spodoptera littoralis