title: Global analysis of cdc14 dephosphorylation sites reveals essential regulatory role in mitosis and cytokinesis.
abstract: Degradation of the M phase cyclins triggers the exit from M phase. Cdc14 is the major phosphatase required for the exit from the M phase. One of the functions of Cdc14 is to dephosphorylate and activate the Cdh1/APC/C complex, resulting in the degradation of the M phase cyclins. However, other crucial targets of Cdc14 for mitosis and cytokinesis remain to be elucidated. Here we systematically analyzed the positions of dephosphorylation sites for Cdc14 in the budding yeast Saccharomyces cerevisiae. Quantitative mass spectrometry identified a total of 835 dephosphorylation sites on 455 potential Cdc14 substrates in vivo. We validated two events, and through functional studies we discovered that Cdc14-mediated dephosphorylation of Smc4 and Bud3 is essential for proper mitosis and cytokinesis, respectively. These results provide insight into the Cdc14-mediated pathways for exiting the M phase.
authors: Department of Microbiology, College of Medicine, National Taiwan University, Taipei 10051, Taiwan;
authors: Li
authors: L
authors: Kao
authors: Yi-Ting
authors: YT
authors: Wang
authors: Yu-Chen
authors: YC
authors: Chen
authors: Shun-Fu
authors: SF
authors: Tseng
authors: Jia-Cin
authors: JC
authors: Jhang
authors: Yu-Ju
authors: YJ
authors: Chen
authors: Shu-Chun
authors: SC
authors: Teng
mesh_terms: D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D020090:Chromosome Segregation; D048749:Cytokinesis / Q000235:genetics; D042003:DNA Packaging; D008938:Mitosis / Q000235:genetics; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: C093319:BUD3 protein, S cerevisiae; C074860:CDC14 protein, S cerevisiae; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D010750:Phosphoproteins; D020543:Proteome; C409162:SMC4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D017027:Protein Tyrosine Phosphatases