title: Purification, crystallization and preliminary X-ray studies of MbtN (Rv1346) from Mycobacterium tuberculosis.
abstract: In Mycobacterium tuberculosis, the protein MbtN (Rv1346) catalyzes the formation of a double bond in the fatty-acyl moiety of the siderophore mycobactin, which is used by this organism to acquire essential iron. MbtN is homologous to acyl-CoA dehydrogenases, whose general role is to catalyze the α,β-dehydrogenation of fatty-acyl-CoA conjugates. Mycobactins, however, contain a long unsaturated fatty-acid chain with an unusual cis double bond conjugated to the carbonyl group of the mycobactin core. To characterize the role of MbtN in the dehydrogenation of this fatty-acyl moiety, the enzyme has been expressed, purified and crystallized. The crystals diffracted to 2.3 Å resolution at a synchrotron source and were found to belong to the hexagonal space group H32, with unit-cell parameters a = b = 139.10, c = 253.09 Å, α = β = 90, γ = 120°.
authors: Structural Biology Laboratory, School of Biological Sciences and Maurice Wilkins Centre for Molecular Biodiscovery, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.
authors: Ai Fen
authors: AF
authors: Chai
authors: Jodie M
authors: JM
authors: Johnston
authors: Richard D
authors: RD
authors: Bunker
authors: Esther M M
authors: EM
authors: Bulloch
authors: Genevieve L
authors: GL
authors: Evans
authors: J Shaun
authors: JS
authors: Lott
authors: Edward N
authors: EN
authors: Baker
mesh_terms: D044944:Acyl-CoA Dehydrogenases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D009169:Mycobacterium tuberculosis / Q000737:chemistry* / Q000235:genetics; D010080:Oxazoles / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D017262:Siderophores / Q000737:chemistry / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D010080:Oxazoles; D011994:Recombinant Proteins; D017262:Siderophores; C018608:mycobactins; D044944:Acyl-CoA Dehydrogenases
keywords: MbtN; Mycobacterium tuberculosis; Rv1346