title: Changes in cellular degradation activity in young and old worker honeybees (Apis mellifera).
abstract: The trophocytes and fat cells of honeybees (Apis mellifera) have been used in cellular senescence studies, but the changes of cellular degradation activity with aging in workers are unknown. In this study, cellular degradation activity was evaluated in the trophocytes and fat cells of young and old workers reared in a field hive. The results showed the following: (1) 20S proteosome activity decreased with aging, whereas its expression increased with aging; (2) the expression of microtubule-associated protein 1 light chain 3-II (LC3-II) and the 70 kD heat shock cognate protein (Hsc70) decreased with aging; (3) the size and number of autophagic vacuoles decreased with aging; (4) p62/SQSTM1 and polyubiquitin aggregate expression decreased with aging; (5) lysosomal efficiency decreased with aging; and (6) molecular target of rapamycin (mTOR) expression increased with aging. These results indicate that young workers have higher levels of cellular degradation activity than old workers and that aging results in a decline in the cellular degradation activity in worker honeybees.
authors: Department of Biomedical Sciences, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan. Electronic address: hsu@mail.cgu.edu.tw.
authors: Chin-Yuan
authors: CY
authors: Hsu
authors: Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan.
authors: Yu-Lung
authors: YL
authors: Chuang
authors: Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan.
authors: Yu-Pei
authors: YP
authors: Chan
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D000375:Aging / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D001343:Autophagy / Q000502:physiology; D001516:Bees / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D016922:Cellular Senescence / Q000502:physiology*; D005216:Fat Body / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D005786:Gene Expression Regulation / Q000502:physiology; D050883:HSC70 Heat-Shock Proteins / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism / Q000473:pathology; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D058570:TOR Serine-Threonine Kinases / Q000096:biosynthesis / Q000235:genetics; D014617:Vacuoles / Q000648:ultrastructure
chemical_list: D050883:HSC70 Heat-Shock Proteins; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D058570:TOR Serine-Threonine Kinases; D046988:Proteasome Endopeptidase Complex
keywords: Aging; Autophagy; Degradation; Honeybees; Ubiquitin