title: Amino acids 78 and 79 of Mammalian Orthoreovirus protein µNS are necessary for stress granule localization, core protein λ2 interaction, and de novo virus replication.
abstract: At early times in Mammalian Orthoreovirus (MRV) infection, cytoplasmic inclusions termed stress granules (SGs) are formed as a component of the innate immune response, however, at later times they are no longer present despite continued immune signaling. To investigate the roles of MRV proteins in SG modulation we examined non-structural protein µNS localization relative to SGs in infected and transfected cells. Using a series of mutant plasmids, we mapped the necessary μNS residues for SG localization to amino acids 78 and 79. We examined the capacity of a μNS(78-79) mutant to associate with known viral protein binding partners of μNS and found that it loses association with viral core protein λ2. Finally, we show that while this mutant cannot support de novo viral replication, it is able to rescue replication following siRNA knockdown of μNS. These data suggest that μNS association with SGs, λ2, or both play roles in MRV replication.
authors: Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011, IA, USA; Program in Genetics, Iowa State University, Ames 50011, IA, USA.
authors: Kate
authors: K
authors: Carroll
authors: Craig
authors: C
authors: Hastings
authors: Cathy L
authors: CL
authors: Miller
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D002460:Cell Line; D003594:Cytoplasmic Granules / Q000821:virology*; D006801:Humans; D012089:Mammalian orthoreovirus 3 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D012088:Reoviridae Infections / Q000821:virology*; D014758:Viral Core Proteins / Q000235:genetics / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000008:administration & dosage* / Q000737:chemistry* / Q000235:genetics; D014779:Virus Replication
chemical_list: D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins
keywords: Innate immunity; Mammalian Orthoreovirus; Replication; Stress granules; Translation; μNS