title: Responses of hepatic metallothioneins and apoptotic activity in Carassius auratus gibelio witness a release of cobalt and zinc from waterborne nanoscale composites.
abstract: The main goal of this study was to evaluate the ability of fish Carassius auratus tissues to release cobalt (Co) and zinc (Zn) cations present in the applied Co- and Zn-containing nanoscale composites (NCs). Male fish was subjected to 14day long action of Co- and Zn-NCs, as well as of Co(2+) and Zn(2+) or polymeric substance (PS) used for the NC preparation and derived from the vinylpyrrolidone. 50μg∙L(-1) of Co and 100μg∙L(-1) of Zn were applied either as a salt or a nanocomposite. Both Co and Co-NC increased (3.1 and 2.3 times, respectively) concentration of total Co, metallothionein-related Co (3.7 and 6.6 times, respectively) and thiols (by 71 and 95%, respectively), and caspase-3 activity (2.2 and 3.7 times, respectively) in the fish liver. At the same time, Co and Co-NC decreased glutathione level (1.8 and 1.9 times, respectively) and activated vitellogenesis (5.1 and 9.9 times, respectively) in the fish liver. Both Zn and Zn-NC increased markedly concentrations of metallothionein-related Zn (2.4 and 2.9 times, respectively) and Cu (2.8 and 3.2 times, respectively), and decreased metallothionein-related thiol (2.5 and 4.2 times, respectively), oxyradical (by 30.4 and 44.2%, respectively), and caspase-3 (3.0 and 5.3 times, respectively) levels in the fish liver. These peculiarities are common for metal and metal-NC and witness a release of metal from NS in fish organism. The differences in the levels of DNA strand breaks, biotransformation enzymes and total Zn levels in the liver were dependent on the kind of exposure.
authors: Ternopil National Pedagogical University (TNPU), Ternopil, Ukraine; I.Ya. Horbachevsky Ternopil State Medical University, Ternopil, Ukraine.
authors: Halina
authors: H
authors: Falfushynska
authors: Ternopil National Pedagogical University (TNPU), Ternopil, Ukraine.
authors: Lesya
authors: L
authors: Gnatyshyna
authors: Ternopil National Pedagogical University (TNPU), Ternopil, Ukraine.
authors: Olga
authors: O
authors: Turta
authors: Ternopil National Pedagogical University (TNPU), Ternopil, Ukraine.
authors: Oksana
authors: O
authors: Stoliar
authors: Lviv National Polytechnic University (LNPU), Lviv, Ukraine.
authors: Natalia
authors: N
authors: Mitina
authors: Lviv National Polytechnic University (LNPU), Lviv, Ukraine.
authors: Alexander
authors: A
authors: Zaichenko
authors: Institute of Cell Biology (ICB), NAS of Ukraine, Lviv, Ukraine. Electronic address: stoika@cellbiol.lviv.ua.
authors: Rostyslav
authors: R
authors: Stoika
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D003035:Cobalt / Q000008:administration & dosage / Q000633:toxicity*; D006054:Goldfish; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008668:Metallothionein / Q000037:antagonists & inhibitors / Q000378:metabolism*; D053761:Nanocomposites / Q000008:administration & dosage / Q000633:toxicity*; D011897:Random Allocation; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity*; D015032:Zinc / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D003035:Cobalt; D008668:Metallothionein; D015032:Zinc
keywords: Apoptosis; Carassius auratus gibelio; Cobalt; Cytotoxicity; Metallothioneins; Oxidative stress; Vinylpyrrolidone-derived nanoscale composite; Zinc