title: Simultaneous determination of β-lactam antibiotics and β-lactamase inhibitors in bovine milk by ultra performance liquid chromatography-tandem mass spectrometry.
abstract: An ultra performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) method has been developed for the simultaneous determination of four β-lactam antibiotics (amoxicillin, ampicillin, cefotaxime, and cefoperazone) and two β-lactamase inhibitors (tazobactam, sulbactam) in bovine milk. The analytes were extracted with water from bovine milk and purified with Oasis HLB solid phase extraction (SPE) cartridges. The analytes were determined in less than 3min by UPLC-MS/MS in positive and negative electrospray ionization (ESI) modes, separately. The method was linear over the range of 1-100μg/L for tazobactam, sulbactam, ampicillin, and cefoperazone, and 2-100μg/L for amoxicillin and cefotaxime. The recoveries for all six analytes in bovine milk ranged from 82.5 to 98.3%. The limits of detection and the limits of quantitation were 0.1-0.2μg/L and 0.3-0.5μg/L, respectively. The intra- and inter-day precisions were less than 6% for each compound.
authors: School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China; Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
authors: Nasi
authors: N
authors: Li
authors: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
authors: Feng
authors: F
authors: Feng
authors: School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
authors: Bingcheng
authors: B
authors: Yang
authors: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
authors: Pingping
authors: P
authors: Jiang
authors: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China. Electronic address: xgchu@vip.sina.com.
authors: Xiaogang
authors: X
authors: Chu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004791:Enzyme Inhibitors / Q000032:analysis*; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods; D065093:beta-Lactamase Inhibitors; D047090:beta-Lactams / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D065093:beta-Lactamase Inhibitors; D047090:beta-Lactams
keywords: Bovine milk; Tandem mass spectrometry; Ultra-high performance liquid chromatography; β-Lactam antibiotics; β-Lactamase inhibitors