title: An on-demand four-way junction DNAzyme nanoswitch driven by inosine-based partial strand displacement.
abstract: A DNA four-way junction device capable of junction expansion and contraction cycles using an inosine-based partial strand displacement scheme is reported. These nanoscale positioning capabilities are used to provide on-demand activation and deactivation of a pair of split E6 DNAzymes on the device. The device also demonstrates a combined catalytic rate significantly higher than the original E6 DNAzyme under similar operational conditions. This approach can provide structural organization and spatially control other multicomponent molecular complexes.
authors: Materials Science and Engineering Program, University of California, 9500 Gilman Drive, La Jolla, San Diego, CA 92093, USA. plandon@ucsd.edu.
authors: Alexander H
authors: AH
authors: Mo
authors: Preston B
authors: PB
authors: Landon
authors: Brian
authors: B
authors: Meckes
authors: Max M
authors: MM
authors: Yang
authors: Gennadi V
authors: GV
authors: Glinsky
authors: Ratnesh
authors: R
authors: Lal
mesh_terms: D002384:Catalysis; D004247:DNA / Q000737:chemistry; D021881:DNA, Catalytic / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D007288:Inosine; D007700:Kinetics; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology / Q000379:methods; D009690:Nucleic Acid Conformation; D013050:Spectrometry, Fluorescence; D013696:Temperature; D013816:Thermodynamics
chemical_list: D021881:DNA, Catalytic; D007288:Inosine; D004247:DNA