title: The role of bone marrow mesenchymal stem cells in the treatment of acute liver failure.
abstract: OBJECTIVE
  This study is to investigate the effects of bone marrow mesenchymal stem cell (BMSC) transplantation on acute liver failure (ALF).
  
  
  METHODS
  BMSCs were separated from rat bone marrow, cultured, and identified by flow cytometry. Rat model with ALF was established by injecting D-galactosamine and lipopolysaccharide. Rats were randomly divided into the control group and BMSC transplantation group. The serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured at 24 h, 120 h, and 168 h after BMSC transplantation. Apoptosis was detected by TUNEL assay. The expression of VEGF and AFP proteins was detected by immunofluorescence. Caspase-1 and IL-18 proteins and mRNA were detected by immunohistochemistry and RT-PCR.
  
  
  RESULTS
  Compared with the control group, levels of ALT, AST, caspase-1 and IL-18 proteins, and mRNA in the transplantation group were significantly lower at 120 h and 168 h after BMSCs transplantation. Apoptosis was inhibited by BMSCs transplantation. The VEGF protein levels were increased with the improvement of liver function, and the AFP protein levels were increased with the deterioration of the liver function after BMSCs transplantation.
  
  
  CONCLUSIONS
  BMSCs transplantation can improve liver function and inhibit hepatocyte apoptosis as well as promote hepatocyte proliferation in rat model with ALF.
authors: Department of Infectious Diseases, First Affiliated Hospital of Xinjiang Medical University, Xinjiang 830011, China ; State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, China.
authors: Shufang
authors: S
authors: Yuan
authors: Tao
authors: T
authors: Jiang
authors: Lihua
authors: L
authors: Sun
authors: Rongjiong
authors: R
authors: Zheng
authors: Nizam
authors: N
authors: Ahat
authors: Yuexin
authors: Y
authors: Zhang
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D017209:Apoptosis; D001219:Aspartate Aminotransferases / Q000097:blood; D001854:Bone Marrow Cells / Q000166:cytology*; D020170:Caspase 1 / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D020287:In Situ Nick-End Labeling; D020382:Interleukin-18 / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000473:pathology; D017114:Liver Failure, Acute / Q000097:blood / Q000201:enzymology / Q000628:therapy*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000378:metabolism
chemical_list: D020382:Interleukin-18; D042461:Vascular Endothelial Growth Factor A; D000509:alpha-Fetoproteins; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D020170:Caspase 1