title: Pharmacokinetics of plantamajoside and acteoside from Plantago asiatica in rats by liquid chromatography-mass spectrometry.
abstract: Plantago asiatica is a medicinal and dietary plant rich in polyphenolic compounds such as phenylpropanoid glycosides plantamajoside and acteoside. The aims of the present study were to develop a new and sensitive method for simultaneous determination of plantamajoside and acteoside and investigate their pharmacokinetic properties in rats. A sensitive and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method with multiple-reaction monitoring (MRM) mode was employed for quantification of two analytes in rat plasma. The calibration curve was linear over the range of 0.2-200ng/ml with correlation coefficient greater than 0.9983 for both analytes. The accuracy of plantamajoside and acteoside were between -4.2% and 8.1%, -3.8% and 8.9% relative error, respectively. Precision for the two analytes ranged from 2.7 to 10.2% relative standard deviation. The pharmacokinetic results showed plantamajoside and acteoside were quickly absorbed in rat with the time to maximum plasma concentration 16.7±2.8 and 13.3±2.8min, respectively. The elimination constants were 0.28±0.01 1h(-1) for plantamajoside and 0.47±0.03 1h(-1) for acteoside. The developed method and the pharmacokinetic data provide a basis for further studies on bioactivity of P. asiatica.
authors: School of Life Science, Beijing Institute of Technology, 5 South Street Zhongguancun, Beijing 100081, China. Electronic address: lylyjlzh2006@yahoo.com.
authors: Yujuan
authors: Y
authors: Li
authors: School of Life Science, Beijing Institute of Technology, 5 South Street Zhongguancun, Beijing 100081, China.
authors: Lin
authors: L
authors: Gan
authors: Faculty of Pharmacy, The University of Sydney, NSW 2006, Australia.
authors: George Q
authors: GQ
authors: Li
authors: School of Life Science, Beijing Institute of Technology, 5 South Street Zhongguancun, Beijing 100081, China.
authors: Li
authors: L
authors: Deng
authors: School of Life Science, Beijing Institute of Technology, 5 South Street Zhongguancun, Beijing 100081, China.
authors: Xinshi
authors: X
authors: Zhang
authors: School of Life Science, Beijing Institute of Technology, 5 South Street Zhongguancun, Beijing 100081, China.
authors: Yulin
authors: Y
authors: Deng
mesh_terms: D000818:Animals; D002396:Catechols / Q000737:chemistry* / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid / Q000379:methods; D005960:Glucosides / Q000737:chemistry* / Q000493:pharmacokinetics*; D008297:Male; D010636:Phenols / Q000737:chemistry* / Q000493:pharmacokinetics*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D010943:Plantago / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods; D059808:Polyphenols
chemical_list: D002396:Catechols; D005960:Glucosides; D010636:Phenols; D010936:Plant Extracts; C421173:plantamajoside; C058956:acteoside; D059808:Polyphenols
keywords: Acteoside; LC–MS; Pharmacokinetics; Plantago asiatica; Plantamajoside