title: Two-color in situ hybridization of whole-mount mouse embryos.
abstract: RNA in situ hybridization is a powerful technique used to identify the spatial localization of a specific RNA in a tissue section or whole tissue. In this protocol, we describe a reliable method for two-color in situ hybridization that can be used to accurately assess the expression of multiple genes with contrasting or overlapping expression patterns in whole mouse embryos.
authors: Frederick National Lab of Cancer Research, NCI, NIH, Frederick, MD, USA.
authors: Kristin K
authors: KK
authors: Biris
authors: Terry P
authors: TP
authors: Yamaguchi
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000166:cytology*; D015870:Gene Expression; D017403:In Situ Hybridization / Q000379:methods*; D051379:Mice; D008967:Molecular Biology / Q000379:methods; D012313:RNA / Q000302:isolation & purification*; D015347:RNA Probes / Q000737:chemistry; D013194:Staining and Labeling
chemical_list: D015347:RNA Probes; D012313:RNA