title: Non-histone protein HMGB1 inhibits the repair of damaged DNA by cisplatin in NIH-3T3 murine fibroblasts.
abstract: The nuclear non-histone protein high mobility group box (HMGB) 1 is known to having an inhibitory effect on the repair of DNA damaged by the antitumor drug cisplatin in vitro. To investigate the role of HMGB1 in living cells, we studied the DNA repair of cisplatin damages in mouse fibroblast cell line, NIH-3T3. We evaluated the effect of the post-synthetic acetylation and C-terminal domain of the protein by overexpression of the parental and mutant GFP fused forms of HMGB1. The results revealed that HMGB1 had also an inhibitory effect on the repair of cisplatin damaged DNA in vivo. The silencing of HMGB1 in NIH-3T3 cells increased the cellular DNA repair potential. The increased levels of repair synthesis could be "rescued" and returned to less than normal levels if the knockdown cells were transfected with plasmids encoding HMGB1 and HMGB1 K2A. In this case, the truncated form of HMGB1 also exhibited a slight inhibitory effect. [BMB Reports 2016; 49(2): 99-104].
authors: Institute of Molecular Biology, Roumen Tsanev, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria.
authors: Shazie
authors: S
authors: Yusein-Myashkova
authors: Institute of Molecular Biology, Roumen Tsanev, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria.
authors: Iva
authors: I
authors: Ugrinova
authors: Institute of Molecular Biology, Roumen Tsanev, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria.
authors: Evdokia
authors: E
authors: Pasheva
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002945:Cisplatin / Q000494:pharmacology*; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D024243:HMGB1 Protein / Q000378:metabolism*; D051379:Mice; D041681:NIH 3T3 Cells
chemical_list: D000970:Antineoplastic Agents; D024243:HMGB1 Protein; D049452:Green Fluorescent Proteins; D002945:Cisplatin