title: Generation of tissue organoids by compaction reaggregation.
abstract: Cellular reaggregation methods are commonly used to generate tissue organoids for use in biological studies. Using a modified method termed "compaction reaggregation," it is possible to establish reaggregates of reproducible size from defined input cell numbers with ease and without specialist equipment. Importantly, this method is suitable for the study of tissues that have proved refractory to reaggregation by other methods. With the option of juxtaposing cell populations, this method is useful for studies of tissue organization and structure.
authors: Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
authors: Julie M
authors: JM
authors: Sheridan
authors: C Clare
authors: CC
authors: Blackburn
mesh_terms: D000818:Animals; D002449:Cell Aggregation / Q000235:genetics*; D051379:Mice; D008967:Molecular Biology / Q000379:methods; D009940:Organoids / Q000254:growth & development*; D013950:Thymus Gland / Q000166:cytology / Q000254:growth & development*