title: Correlation between the expression levels of miR-1 and PIK3CA in non-small-cell lung cancer and their relationship with clinical characteristics and prognosis.
abstract: OBJECTIVE
  To examine the relationship between the expression levels of miR-1 and PIK3CA in resected non-small-cell lung cancer (NSCLC) tissues and clinical characteristics and prognosis.
  
  
  METHODS
  Levels of miR-1 and PIK3CA in cancerous and normal tissues obtained from 55 patients with NSCLC were measured by real-time PCR and western blot assays, and their associations with clinicopathological features were evaluated.
  
  
  RESULTS
  A total of 69.1% of the NSCLC tissue samples showed low miR-1 expression (p < 0.05) and 70.9% showed high PIK3CA expression (p < 0.05). Patients with low miR-1 expression and those with high PIK3CA expression had significantly higher incidences of lymph node metastases and postoperative recurrences within 1 year after surgery than those with moderate miR-1 expression and moderate PIK3CA expression (p < 0.05 for both).
  
  
  CONCLUSIONS
  Low expression of miR-1 and overexpression of PIK3CA in NSCLC tissues may be useful predictors of lymph node metastasis and postoperative recurrence in patients with NSCLC.
authors: Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou Road, Nanjing 210029, Jiangsu, People's Republic of China.
authors: Qiu
authors: Q
authors: Zhao
authors: Bin
authors: B
authors: Zhang
authors: Yongfeng
authors: Y
authors: Shao
authors: Liang
authors: L
authors: Chen
authors: Xuanyi
authors: X
authors: Wang
authors: Zhihong
authors: Z
authors: Zhang
authors: Yongqian
authors: Y
authors: Shu
authors: Renhua
authors: R
authors: Guo
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D058534:Class I Phosphatidylinositol 3-Kinases; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D008207:Lymphatic Metastasis / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics* / Q000378:metabolism; D011237:Predictive Value of Tests; D011379:Prognosis; D012016:Reference Values
chemical_list: C544547:MIRN1 microRNA, human; D035683:MicroRNAs; D019869:Phosphatidylinositol 3-Kinases; D058534:Class I Phosphatidylinositol 3-Kinases; C484760:PIK3CA protein, human