title: Human adipose-derived stem cells modified by HIF-1α accelerate the recovery of cisplatin-induced acute renal injury in vitro.
abstract: Human adipose-derived stem cells (hASCs) improve renal function in acute kidney injury. Hypoxia-inducible factor-1α (HIF-1α) was transfected into hASCs. hASCs modified by lentivirus-mediated empty-vector and HIF-1α maintained their stem cell characteristics. The expression of the renal-protective gene, heme oxygenase-1 and vascular endothelial growth factor were significantly increased in hASCs modified by HIF-1α, compared to hASCs modified by empty-vector. Cellular ultra-structure and TUNEL staining revealed that hASCs modified by HIF-1α promoted the recovery of apoptotic morphology in cisplatin-treated human kidney-2 cells (HK-2 cells) when compared to hASCs modified by empty-vector. Additionally, hASCs modified by empty-vector inhibited caspase-3 expression and up-regulated Bcl-2 expression in cisplatin-treated HK-2 cells, an effect even more pronounced with hASCs modified by HIF-1α. Thus, HIF-1α gene-modified ASCs could be an effective way to enhance the renal-protective effect.
authors: Department of Nephrology, Jimin Hospital, Shanghai, 200052, People's Republic of China, w.vwei@163.com.
authors: Weiwei
authors: W
authors: Wang
authors: Wei
authors: W
authors: Wang
authors: Yan
authors: Y
authors: Jiang
authors: Zezheng
authors: Z
authors: Li
authors: Jin
authors: J
authors: Cheng
authors: Nanmei
authors: N
authors: Liu
authors: Guofeng
authors: G
authors: Han
authors: Shi
authors: S
authors: Lu
authors: Jinyuan
authors: J
authors: Zhang
mesh_terms: D058186:Acute Kidney Injury / Q000628:therapy*; D017209:Apoptosis; D002460:Cell Line; D017690:Cell Transplantation / Q000379:methods*; D002945:Cisplatin / Q000633:toxicity*; D004847:Epithelial Cells / Q000502:physiology; D005822:Genetic Vectors; D051547:Heme Oxygenase-1 / Q000378:metabolism; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D066298:In Vitro Techniques; D016086:Lentivirus / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A; D051547:Heme Oxygenase-1; D002945:Cisplatin