title: [Effect of neonatal immunization with different dosage allergen on the immunity of mice when grown up].
abstract: OBJECTIVE
  To study the effect of neonatal immunization with different dosage allergen on the immunity of mice when grown.
  
  
  METHODS
  Fifty neonatal BALB/c mice were divided into 4 groups randomly and subcutaneous injected with different dosage of ovalbumin (OVA) on day 1, 8 and 15 after born [NS group(10): injected with saline alone; NS + AL group (10): injected with saline and AL(OH)3; small dosage (SD) group (15): injected with 10 microg OVA and AL(OH)3; large dosage (LD) group (15): injected with 1000 microg OVA and AL(OH)3]. The mice were then challenged using caudal vein injection on 5 weeks old (NS group and NS + AL group were challenged with saline, SD group and LD group were challenged with 100 microg OVA). The blood was collected 1 week later to examine OVA specific IgE, IgG1 and IgG2a. Mononuclear cells were drawn from the spleen and cultured. Concentration of IL-4, IFN-r, IL-10 was examined in the cultural supernatant. Flow cytometry was used to test the expression of CD4+ IL-4+, CD4+ IFN-gamma+, CD4 IL-10 cells.
  
  
  RESULTS
  It was found that concentration of OVA specific IgE (OVA-sIgE) in SD group (0.33 +/- 0.18) was significantly higher than that of NS (0.07 +/- 0.01) and NS + AL (0.09 +/- 0.04) group (t value was -3.46 and -3.21, all P < 0.01), and LD group (0.17 +/- 0.10) as well (t = 2.58, P < 0.05). The concentration of OVA-sIgE was higher in LD group than that of NS group (t = -2.53, P < 0.05), but similar with that of NS + AL group (t = -2.04, P > 0.05). Both the concentration of OVA-sIgG1 and sIgG2a was higher in SD and LD group than that of NS and NS + AL group (all P < 0.05). The concentration of IL-4, IFN-gamma and IL-10 in the cultural supernatant of spleen mononuclear was all higher in SD group than that of NS and NS + AL group (all P < 0.01). The ratio of IFN-gamma/IL-4 was significantly lower in SD group than that of NS and NS + AL group (t value was 2.14, 3.44, all P < 0.05), while the same ratio was higher in LD group than that of NS and NS + AL group (t value was -2.14, -1.61, all P < 0.05). Ratio of CD4+ IL-4+ cells was significant lower in LD group than that of SD group (P < 0.05), while it was not different with that of NS and NS + AL group (P > 0. 05).
  
  
  CONCLUSIONS
  Neonatal immunization with low dosage OVA could generate a specific immunity with Th2 direction, while with large dosage OVA could generate a specific immunity with Th1 direction.
authors: Department of Otorhinolaryngology , Union Hospital of Tongji Medical college, Huazhong University of Science and Technology, Wuhan 430022, China. ylly80331@163.com
authors: Jian-Jun
authors: JJ
authors: Chen
authors: Jing-Hui
authors: JH
authors: Wang
authors: Yang
authors: Y
authors: Yuan
authors: Jun-Mei
authors: JM
authors: Fu
authors: Zi-Zhong
authors: ZZ
authors: Yu
authors: Yan-Jun
authors: YJ
authors: Wang
authors: Wei-Jia
authors: WJ
authors: Kong
mesh_terms: D000485:Allergens / Q000008:administration & dosage*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes; D004305:Dose-Response Relationship, Drug; D007114:Immunization; D007074:Immunoglobulin G / Q000378:metabolism; D007371:Interferon-gamma; D016753:Interleukin-10 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage
chemical_list: D000485:Allergens; D007074:Immunoglobulin G; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma; D010047:Ovalbumin