title: Effect of 940 nm low-level laser therapy on osteogenesis in vitro.
abstract: Bone regeneration is essential in medical treatment, such as in surgical bone healing and orthodontics. The aim of this study is to examine the effect of different powers of 940 nm diode low-level laser treatment (LLLT) on osteoblast cells during their proliferation and differentiation stages. A human fetal osteoblast cell line was cultured and treated with LLLT. The cells were divided into experimental groups according to the power delivered and periods of exposure per day for each laser power. The (3-(4,5-dimethylthiazol-2yl)-2,5 diphenyl tetrazolium bromide) (MTT) assay was used to determine cell proliferation. Both alkaline phosphatase and osteocalcin activity assays were assessed for cell differentiation. All treatment groups showed a significant increase in cell proliferation and differentiation compared to the control group. Regarding the exposure time, the subgroups treated with the LLLT for 6 min showed higher proliferation and differentiation rates for the powers delivered, the 300-mW LLLT group significantly increased the amount of cell proliferation. By contrast, the 100 and 200 mW groups showed significantly greater amounts of cell differentiation. These results suggest that the use of LLLT may play an important role in stimulating osteoblast cells for improved bone formation.
authors: Universiti Sains Malaysia, School of Dental Sciences, Health Campus, Orthodontic Unit, Kota Bharu, Kelantan 16150, MalaysiaeAlnahrain University, Baghdad, Iraq.
authors: Mohammed Mahmood
authors: MM
authors: Jawad
authors: Universiti Sains Malaysia, School of Dental Sciences, Health Campus, Conservative Department, Kota Bharu, Kelantan 16150, Malaysia.
authors: Adam
authors: A
authors: Husein
authors: Universiti Sains Malaysia, School of Dental Sciences, Health Campus, Kota Bharu, Kelantan 16150, Malaysia.
authors: Ahmad
authors: A
authors: Azlina
authors: Universiti Sains Malaysia, School of Dental Sciences, Health Campus, Orthodontic Unit, Kota Bharu, Kelantan 16150, Malaysia.
authors: Mohammad Khursheed
authors: MK
authors: Alam
authors: Universiti Sains Malaysia, School of Dental Sciences, Health Campus, Orthodontic Unit, Kota Bharu, Kelantan 16150, Malaysia.
authors: Rozita
authors: R
authors: Hassan
authors: Universiti Malaysia Kelantan, Clinical sciences, Faculty of Veterinary Medicine, Kota Bharu, Kelantan 16100, Malaysia.
authors: Rumaizi
authors: R
authors: Shaari
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism; D002454:Cell Differentiation / Q000528:radiation effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000528:radiation effects*; D006801:Humans; D054023:Lasers, Semiconductor; D028022:Low-Level Light Therapy; D010006:Osteoblasts / Q000528:radiation effects*; D015675:Osteocalcin / Q000032:analysis / Q000378:metabolism; D010012:Osteogenesis
chemical_list: D015675:Osteocalcin; D000469:Alkaline Phosphatase