title: Autophagy proteins stabilize pathogen-containing phagosomes for prolonged MHC II antigen processing.
abstract: Antigen preservation for presentation is a hallmark of potent antigen-presenting cells. In this paper, we report that in human macrophages and dendritic cells, a subset of phagosomes gets coated with Atg8/LC3, a component of the molecular machinery of macroautophagy, and maintains phagocytosed antigens for prolonged presentation on major histocompatibility complex class II molecules. These Atg8/LC3-positive phagosomes are formed around the antigen with TLR2 agonists and require reactive oxygen species production by NOX2 for their generation. A deficiency in the NOX2-dependent formation of these antigen storage phagosomes could contribute to compromise antifungal immune control in chronic granulomatous disease patients.
authors: Viral Immunobiology, Institute of Experimental Immunology, and 2 Center for Microscopy and Image Analysis, University of Zürich, 8006 Zürich, Switzerland.
authors: Susana
authors: S
authors: Romao
authors: Nathalie
authors: N
authors: Gasser
authors: Andrea C
authors: AC
authors: Becker
authors: Bruno
authors: B
authors: Guhl
authors: Milica
authors: M
authors: Bajagic
authors: Danusia
authors: D
authors: Vanoaica
authors: Urs
authors: U
authors: Ziegler
authors: Joachim
authors: J
authors: Roesler
authors: Jörn
authors: J
authors: Dengjel
authors: Janine
authors: J
authors: Reichenbach
authors: Christian
authors: C
authors: Münz
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D017951:Antigen Presentation; D001343:Autophagy / Q000502:physiology*; D000071190:Autophagy-Related Protein 8 Family; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism / Q000502:physiology; D008840:Microfilament Proteins / Q000378:metabolism*; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000378:metabolism / Q000502:physiology; D010588:Phagosomes / Q000378:metabolism* / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000071190:Autophagy-Related Protein 8 Family; C498698:GABARAPL2 protein, human; D000949:Histocompatibility Antigens Class II; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D017382:Reactive Oxygen Species; C056558:CYBB protein, human; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases