title: The effect of 17α-ethynylestradiol on steroidogenesis and gonadal cytokine gene expression is related to the reproductive stage in marine hermaphrodite fish.
abstract: Pollutants have been reported to disrupt the endocrine system of marine animals, which may be exposed through contaminated seawater or through the food chain. Although 17α-ethynylestradiol (EE₂), a drug used in hormone therapies, is widely present in the aquatic environment, current knowledge on the sensitivity of marine fish to estrogenic pollutants is limited. We report the effect of the dietary intake of 5 µg EE₂/g food on different processes of testicular physiology, ranging from steroidogenesis to pathogen recognition, at both pre-spermatogenesis (pre-SG) and spermatogenesis (SG) reproductive stages, of gilthead seabream (Sparus aurata L.), a marine hermaphrodite teleost. A differential effect between pre-SG and SG specimens was detected in the sex steroid serum levels and in the expression profile of some steroidogenic-relevant molecules, vitellogenin, double sex- and mab3-related transcription factor 1 and some hormone receptors. Interestingly, EE₂ modified the expression pattern of some immune molecules involved in testicular physiology. These differences probably reflect a developmental adjustment of the sensitivity to EE₂ in the gilthead seabream gonad.
authors: Isabel
authors: I
authors: Cabas
authors: Elena
authors: E
authors: Chaves-Pozo
authors: Alicia
authors: A
authors: García-Alcázar
authors: José
authors: J
authors: Meseguer
authors: Victoriano
authors: V
authors: Mulero
authors: Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Murcia 30100, Spain. agayala@um.es.
authors: Alfonsa
authors: A
authors: García-Ayala
mesh_terms: D000818:Animals; D016207:Cytokines / Q000276:immunology; D004997:Ethinyl Estradiol / Q000009:adverse effects* / Q000276:immunology; D005399:Fishes / Q000276:immunology; D015870:Gene Expression / Q000187:drug effects / Q000276:immunology; D012739:Gonadal Steroid Hormones / Q000276:immunology / Q000378:metabolism*; D006066:Gonads / Q000187:drug effects* / Q000276:immunology; D008297:Male; D012098:Reproduction / Q000187:drug effects* / Q000276:immunology; D021541:Sea Bream / Q000276:immunology; D013081:Sperm Motility / Q000187:drug effects / Q000276:immunology; D013091:Spermatogenesis / Q000187:drug effects* / Q000276:immunology; D013737:Testis / Q000187:drug effects / Q000276:immunology; D014874:Water Pollutants, Chemical / Q000009:adverse effects / Q000276:immunology
chemical_list: D016207:Cytokines; D012739:Gonadal Steroid Hormones; D014874:Water Pollutants, Chemical; D004997:Ethinyl Estradiol