title: In vitro susceptibility of Malassezia pachydermatis isolates from canine skin with atopic dermatitis to ketoconazole and itraconazole in East Asia.
abstract: Topical or oral azole antifungals are commonly used in canine atopic dermatitis (AD), as the lipophilic yeast Malassezia pachydermatis exacerbates canine AD. To examine whether canine AD lesions harbor azole-resistant M. pachydermatis isolates in East Asia, we investigated the in vitro susceptibility of M. pachydermatis isolates to ketoconazole (KTZ) and itraconazole (ITZ) obtained from AD lesions of canines in Japan, Korea and Taiwan. The minimum inhibitory concentrations (MICs) of KTZ and ITZ were measured by the E-test using Sabouraud dextrose agar with 0.5% Tween 40. The MICs of KTZ and ITZ for isolates from canines with AD were significantly higher than the MICs for isolates from healthy canines. Our findings suggested that the clinical isolates from canine AD skin lesions were less susceptible to azoles than those from normal canine skin in East Asia.
authors: Department of Pathobiology, Nihon University School of Veterinary Medicine, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors: Shion
authors: S
authors: Watanabe
authors: Anna
authors: A
authors: Koike
authors: Rui
authors: R
authors: Kano
authors: Masahiko
authors: M
authors: Nagata
authors: Charles
authors: C
authors: Chen
authors: Cheol-Yong
authors: CY
authors: Hwang
authors: Atsuhiko
authors: A
authors: Hasegawa
authors: Hiroshi
authors: H
authors: Kamata
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology* / Q000627:therapeutic use; D003881:Dermatomycoses / Q000188:drug therapy / Q000662:veterinary*; D004283:Dog Diseases / Q000188:drug therapy* / Q000382:microbiology*; D004285:Dogs; D025141:Drug Resistance, Fungal / Q000502:physiology; D005202:Asia, Eastern; D017964:Itraconazole / Q000494:pharmacology* / Q000627:therapeutic use; D007654:Ketoconazole / Q000494:pharmacology* / Q000627:therapeutic use; D008290:Malassezia / Q000187:drug effects*; D008826:Microbial Sensitivity Tests
chemical_list: D000935:Antifungal Agents; D017964:Itraconazole; D007654:Ketoconazole