title: Human gut microbiome and risk for colorectal cancer.
abstract: We tested the hypothesis that an altered community of gut microbes is associated with risk of colorectal cancer (CRC) in a study of 47 CRC case subjects and 94 control subjects. 16S rRNA genes in fecal bacterial DNA were amplified by universal primers, sequenced by 454 FLX technology, and aligned for taxonomic classification to microbial genomes using the QIIME pipeline. Taxonomic differences were confirmed with quantitative polymerase chain reaction and adjusted for false discovery rate. All statistical tests were two-sided. From 794217 16S rRNA gene sequences, we found that CRC case subjects had decreased overall microbial community diversity (P = .02). In taxonomy-based analyses, lower relative abundance of Clostridia (68.6% vs 77.8%) and increased carriage of Fusobacterium (multivariable odds ratio [OR] = 4.11; 95% confidence interval [CI] = 1.62 to 10.47) and Porphyromonas (OR = 5.17; 95% CI = 1.75 to 15.25) were found in case subjects compared with control subjects. Because of the potentially modifiable nature of the gut bacteria, our findings may have implications for CRC prevention.
authors: Affiliations of authors: Division of Epidemiology, Department of Population Health (JA, CD, JW, RBH), Department of Pathology (ZP), and Department of Medicine (ZP, LY), New York University School of Medicine, New York, NY; New York University Cancer Institute, New York, NY (JA, ZP, CD, JW, RBH); Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD (RS, JS, JJG); Department of Pathology and Laboratory Medicine, New York Veterans Affairs Medical Center, New York, NY (ZP).
authors: Jiyoung
authors: J
authors: Ahn
authors: Rashmi
authors: R
authors: Sinha
authors: Zhiheng
authors: Z
authors: Pei
authors: Christine
authors: C
authors: Dominianni
authors: Jing
authors: J
authors: Wu
authors: Jianxin
authors: J
authors: Shi
authors: James J
authors: JJ
authors: Goedert
authors: Richard B
authors: RB
authors: Hayes
authors: Liying
authors: L
authors: Yang
mesh_terms: D003013:Clostridium / Q000235:genetics / Q000302:isolation & purification; D015179:Colorectal Neoplasms / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005673:Fusobacterium / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D064307:Microbiota; D016133:Polymerase Chain Reaction; D017861:Porphyromonas / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000302:isolation & purification
chemical_list: D012336:RNA, Ribosomal, 16S