title: One-step homogeneous non-stripping chemiluminescence metal immunoassay based on catalytic activity of gold nanoparticles.
abstract: The catalytic activity of gold nanoparticles (AuNPs) on a luminol-H2O2 chemiluminescence (CL) system is found to be greatly enhanced after its crosslinking aggregation induced by immunoreaction. Based on this observation, a one-step homogeneous non-stripping CL metalloimmunoassay was designed. In the presence of corresponding antigen (Ag), the immunoreaction caused the aggregation of antibody (Ab)-modified AuNPs, and these crosslinking aggregated AuNPs could catalyze luminol-H2O2 CL reaction to produce a much stronger CL signal than dispersed Ab-modified AuNPs. The assay, including immunoreaction and detection, can be accomplished in homogeneous solution. In the assay, no tedious and strict stripping of metal nanoparticles, difficult synthesis of labels, multiple steps of immunoreactions and washings, and complicated magnetic separation process were required. The detection limit of human immunoglobulin G (IgG, 3σ) was estimated to be as low as 3.2×10(-11) g ml(-1). The sensitivity was increased by two orders of magnitude over that of other AuNP-based CL immunoassay. The current CL metalloimmunoassay offers the advantages of being simple, cheap, rapid, and sensitive.
authors: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, People's Republic of China; Department of Environment and Equipment Engineering, Fujian University of Technology, Fuzhou 350108, People's Republic of China.
authors: Yingying
authors: Y
authors: Qi
authors: Department of Environment and Equipment Engineering, Fujian University of Technology, Fuzhou 350108, People's Republic of China.
authors: Fu-Rong
authors: FR
authors: Xiu
authors: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, People's Republic of China. Electronic address: libaoxin@snnu.edu.cn.
authors: Baoxin
authors: B
authors: Li
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis*; D057230:Limit of Detection; D049408:Luminescent Agents / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D007074:Immunoglobulin G; D049408:Luminescent Agents; D008165:Luminol; D006046:Gold
keywords: Chemiluminescence; Gold nanoparticles; Homogeneous; Immunoassay; Non-stripping