title: Tacolimus postconditioning alleviates apoptotic cell death in rats after spinal cord ischemia-reperfusion injury via up-regulating protein-serine-threonine kinases phosphorylation.
abstract: The effects of tacrolimus postconditioning on protein-serine-threonine kinases (Akt) phosphorylation and apoptotic cell death in rats after spinal cord ischemia-reperfusion injury were investigated. Ninety male SD rats were randomly divided into sham operation group, ischemia-reperfusion group and tacrolimus postconditioning group. The model of spinal cord ischemia was established by means of catheterization through femoral artery and balloon dilatation. The spinal cord was reperfused 20 min after ischemia via removing saline out of balloon. The corresponding spinal cord segments were excised and determined for Akt activity in spinal cord tissue by using Western blotting at 5, 15, and 60 min after reperfusion respectively. Spinal cord tissue sections were stained immunohistochemically for detection of the phosphorylated Akt expression at 15 min after reperfusion. Flow cytometry was applied to assess apoptosis of neural cells, and dry-wet weights method was employed to measure water content in spinal cord tissue at 24 h after reperfusion. The results showed that the activities of Akt in tarcolimus postconditioning group were significantly higher than those in ischemia-reperfusion group at 5, 15, and 60 min after reperfusion (P<0.05, P<0.01). The Akt activities reached the peak at 15 min after reperfusion in ischemia-reperfusion group and tacrolimus postconditioning group. The percentage of apoptotic cells and water content in spinal cord tissue were significantly reduced (P<0.01) in tacrolimus postconditioning group as compared with those in ischemia-reperfusion group at 24 h after reperfusion. It is concluded that tacrolimus post-conditioning can increase Akt activity in spinal cord tissue of rats, inhibit apoptosis of neural cells as well as tissue edema, and thereby alleviate spinal cord ischemia-reperfusion injury.
authors: Department of Spine Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors: Feng
authors: F
authors: Pan
authors: Department of Gynaecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors: Yan-Xiang
authors: YX
authors: Cheng
authors: Department of Laboratory Medicine, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors: Cheng-Liang
authors: CL
authors: Zhu
authors: Department of Spine Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors: Feng-Hua
authors: FH
authors: Tao
authors: Department of Spine Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China. lzh999999@yahoo.com.cn.
authors: Zhang-Hua
authors: ZH
authors: Li
authors: Department of Spine Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors: Hai-Ying
authors: HY
authors: Tao
authors: Department of Spine Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors: Bin
authors: B
authors: He
authors: Department of Spine Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors: Ling
authors: L
authors: Yu
authors: Department of Spine Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors: Peng
authors: P
authors: Ji
authors: Department of Spine Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors: Huan
authors: H
authors: Tang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D007166:Immunosuppressive Agents / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D020760:Spinal Cord Ischemia / Q000188:drug therapy* / Q000378:metabolism; D016559:Tacrolimus / Q000494:pharmacology / Q000627:therapeutic use*; D015854:Up-Regulation
chemical_list: D007166:Immunosuppressive Agents; D012333:RNA, Messenger; D017346:Protein Serine-Threonine Kinases; D016559:Tacrolimus