title: Flavonoids from Podocarpus macrophyllus and their cardioprotective activities.
abstract: One new 8-aryl flavone, podocarflavone A (1), together with 15 previously reported flavonoids were isolated from the twigs and leaves of Podocarpus macrophyllus. Their structures were established on the basis of extensive spectroscopic analysis and by the comparison with spectroscopic data reported in the literature. Antioxidant capacities of the isolated substances were determined using the 1,1-diphenyl-2-picrylhydrazyl, ferrous ions, and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical in vitro assays, and their cytoprotective activities were also tested on H2O2-induced apoptosis in H9c2 cardiomyocytes. The results showed that those flavonoids exhibited significant cardioprotective effects by decreasing the H2O2-induced death of H9c2 cell, and the levels of lactate dehydrogenase and creatine kinase, and by inhibiting the elevated intracellular concentration of reactive oxygen species.
authors: a Department of Traditional Chinese Medicine , Qilu Hospital, Shandong University , Jinan , 250012 , China.
authors: Yun
authors: Y
authors: Qiao
authors: Wei-Wei
authors: WW
authors: Sun
authors: Jian-Feng
authors: JF
authors: Wang
authors: Ji-Dong
authors: JD
authors: Zhang
mesh_terms: D000465:Algorithms; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001713:Biphenyl Compounds / Q000494:pharmacology; D002316:Cardiotonic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015394:Molecular Structure; D010851:Picrates / Q000494:pharmacology; D028201:Pinaceae / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D002316:Cardiotonic Agents; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D016166:Free Radical Scavengers; D010851:Picrates; D017382:Reactive Oxygen Species; C587225:podocarflavone A; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl