title: Characterization of a novel l-amino acid oxidase with protein oxidizing activity from Penicillium steckii AIU 027.
abstract: An enzyme exhibiting oxidase activity for β-lactoglobulin, myoglobin, and l-lysine-containing peptides was found from a newly isolated fungal strain, Penicillium steckii AIU 027. The enzyme also oxidized l-amino acids, N(α)-benzyloxycarbonyl-l-lysine (N(α)-Z-l-lysine) and N(ε)-Z-l-lysine, but not d-amino acids and amines. Thus, the enzyme was classified into a group of l-amino acid oxidases (l-AAOs). However, characteristics of this l-AAO were significantly different from those of other l-AAOs as follows. The l-AAO from P. steckii AIU 027 oxidized both the α-amino group and the ε-amino group in l-amino acids and l-lysine-containing peptides, and the Km values for l-lysine-containing polypeptides were lower than those for N(α)-Z-l-lysine and l-lysine-containing dipeptides. The enzyme contained flavin and iron, and composed of four identical subunits with molecular mass of 75.3 kDa. The N-terminal amino acid sequence, ENIADVADAMGPWFDGVAYMKSKKN, was different from that of other l-AAOs. Thus, the l-AAO with protein oxidase activity was first reported here from P. steckii AIU 027.
authors: Department of Biological Chemistry and Food Science, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan. Electronic address: kiso@iwate-u.ac.jp.
authors: Kimiyasu
authors: K
authors: Isobe
authors: Department of Biological Chemistry and Food Science, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan.
authors: Ryota
authors: R
authors: Taira
authors: Department of Biological Chemistry and Food Science, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan.
authors: Youko
authors: Y
authors: Hoshi
authors: Department of Biological Chemistry and Food Science, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan.
authors: Sou
authors: S
authors: Matsuda
authors: Department of Biological Chemistry and Food Science, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan.
authors: Miwa
authors: M
authors: Yamada
authors: Division of Applied Life Science, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.
authors: Makoto
authors: M
authors: Hibi
authors: Division of Applied Life Science, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.
authors: Shigenobu
authors: S
authors: Kishino
authors: Division of Applied Life Science, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.
authors: Jun
authors: J
authors: Ogawa
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D004138:Dinitrocresols / Q000737:chemistry; D004790:Enzyme Induction; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D007700:Kinetics; D050830:L-Amino Acid Oxidase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010407:Penicillium / Q000201:enzymology* / Q000378:metabolism; D020539:Sequence Analysis, Protein; D013379:Substrate Specificity
chemical_list: D000596:Amino Acids; D004138:Dinitrocresols; D005656:Fungal Proteins; C024132:4,6-dinitro-o-cresol; D050830:L-Amino Acid Oxidase
keywords: Lysyl oxidase; Penicillium steckii; Protein oxidase; l-Amino acid oxidase; l-Lysine