title: Alpha-actinin is a new type of house dust mite allergen.
abstract: Main indoor allergens for humans are from house dust mites. There are more than 30 allergens in Dermatophagoides farinae but only fourteen allergens have been identified from this mite including Der f 1-3, 6, 7, 10, 11, 13-18, and 22. A native allergen protein (Der f 24, 90 kDa) was purified from D. farinae by gel filtration and anionic exchange liquid chromatography combined with IgE immunodetection. Its primary structure was determined by Edman degradation, mass spectrometry analysis and cDNA cloning. Enzyme-linked immunosorbent assay inhibition tests (ELISA-IT), immunoblots, basophil activation test (BAT) and skin prick test (SPT) were performed to evaluate the allergenicity. It was identified as an alpha (α)-actinin containing a CaM-like domain with EF-hand motifs. Der f 24 reacted to sera from 85.4% (35/41) of patients on western blot analysis. It reduced ∼20% sera IgE reactivity to D. farinae extracts on a competitive ELISA. Eighty percent (8/10) of patients with D. farinae allergy showed positive reactions to Der f 24 in skin prick test. The expression of CD63 on basophils from patients was up-regulated by Der f 24 by ∼5.4-fold. Alpha-actinin was identified as a new type of house dust mite allergen. To the best of our knowledge, this is the first report of α-actinin as an allergen.
authors: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, China ; Graduate School of the Chinese Academy of Sciences, Beijing, China.
authors: Su
authors: S
authors: An
authors: Chuanbing
authors: C
authors: Shen
authors: Xiaoyu
authors: X
authors: Liu
authors: Lingling
authors: L
authors: Chen
authors: Xuemei
authors: X
authors: Xu
authors: Mingqiang
authors: M
authors: Rong
authors: Zhigang
authors: Z
authors: Liu
authors: Ren
authors: R
authors: Lai
mesh_terms: D000185:Actinin / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D001483:Base Sequence; D001491:Basophils / Q000276:immunology; D002457:Cell Extracts; D018076:DNA, Complementary / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000097:blood / Q000378:metabolism; D008875:Middle Aged; D008969:Molecular Sequence Data; D008970:Molecular Weight; D039981:Pyroglyphidae / Q000276:immunology*; D012882:Skin Tests; D021241:Spectrometry, Mass, Electrospray Ionization; D055815:Young Adult
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D002457:Cell Extracts; D018076:DNA, Complementary; D000185:Actinin; D007073:Immunoglobulin E