title: [Genetic characterization of G II.12 norovirus in Beijing from 2008 to 2009].
abstract: OBJECTIVE
  To reveal the genetic characteristics of GII.12 Norovirus strains isolating from stool samples of adults with diarrhea in Beijing during 2008-2009.
  
  
  METHODS
  RdRp, ORF2, ORF3 and ORF1/ORF2 overlap region were respectively amplified by primers using RT-PCR. The products were purified, cloned, sequenced and then aligned, phylogenetic and recombinant analyzed by softwares of DNAStar, MEGA and SimPlot.
  
  
  RESULTS
  According to the phylogenetic analysis, 11 strains belonged to G II.g in the RdRp region,while GII.12 in the ORF2 and ORF3. SimPlot analysis further confirmed the 11 strains were recombinant strains ( G II.g [RdRp]/G II.12 [capsid]).
  
  
  CONCLUSIONS
  G II.12 Norovirus prevailing in Beijing and other regions of the world belonged to the same strain, and we identified the genetic characteristics of G II.12 Norovirus in Beijing.
authors: Wenzhou Medical University, Zhejiang 317500, China.
authors: Ke-Na
authors: KN
authors: Chen
authors: Geng
authors: G
authors: Tian
authors: Miao
authors: M
authors: Jin
authors: Hui-Ying
authors: HY
authors: Li
authors: Quan-Rui
authors: QR
authors: Li
authors: Li-Hong
authors: LH
authors: Kang
authors: Cui-Hong
authors: CH
authors: Zhang
authors: Xiang-Yu
authors: XY
authors: Kong
authors: Ji-In
authors: JI
authors: Gao
authors: Zhao-Jun
authors: ZJ
authors: Duan
mesh_terms: D002681:China; D029322:Norovirus / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D011995:Recombination, Genetic; D013997:Time Factors