title: Acute ablation of DP thymocytes induces up-regulation of IL-22 and Foxn1 in TECs.
abstract: Thymic epithelial cells (TECs) provide the basic architecture for the development of thymocytes. TEC is regenerative after impairment in thymus of young mice. However, how this regeneration program is governed remains unclear. Transcription factor Foxn1 is a central mediator of the differentiation and function of TEC. We examined the relation between thymic injury and Foxn1 in TEC. Total body irradiation (TBI) treatments induced up-regulation of Foxn1 in TEC, which was abolished when thymic function recovered. Specific depletion of double positive (DP) thymocytes triggered the up-regulation of Foxn1. On the other hand, extracellular IL-22 is a potential regulator of homeostasis of TEC. We demonstrated that TBI treatments also induced the up-regulation of intrathymic IL-22. Expression pattern of Foxn1 shares similar characteristics with IL-22. Furthermore, Foxn1 related genes that regulate the function of TEC were also up-regulated. Thus, our data reveal that TBI treatment triggers regeneration program of TEC.
authors: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, China.
authors: Bin
authors: B
authors: Pan
authors: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, China.
authors: Jie
authors: J
authors: Liu
authors: Laboratory of Transplant Immunology, Xuzhou Medical College, Xuzhou, China.
authors: Ying
authors: Y
authors: Zhang
authors: Laboratory of Transplant Immunology, Xuzhou Medical College, Xuzhou, China.
authors: Yuqi
authors: Y
authors: Sun
authors: Laboratory of Transplant Immunology, Xuzhou Medical College, Xuzhou, China.
authors: Qingyun
authors: Q
authors: Wu
authors: Laboratory of Transplant Immunology, Xuzhou Medical College, Xuzhou, China.
authors: Kai
authors: K
authors: Zhao
authors: Laboratory of Transplant Immunology, Xuzhou Medical College, Xuzhou, China.
authors: Lingyu
authors: L
authors: Zeng
authors: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, China; Laboratory of Transplant Immunology, Xuzhou Medical College, Xuzhou, China. Electronic address: xukl_xzmc@163.com.
authors: Kailin
authors: K
authors: Xu
mesh_terms: D000818:Animals; D002460:Cell Line; D003907:Dexamethasone; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D051858:Forkhead Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D007378:Interleukins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D060168:Thymocytes / Q000378:metabolism* / Q000528:radiation effects; D013950:Thymus Gland / Q000293:injuries / Q000378:metabolism; D015854:Up-Regulation; D014916:Whole-Body Irradiation; D000097043:Interleukin-22
chemical_list: D003907:Dexamethasone; D051858:Forkhead Transcription Factors; D007378:Interleukins; D000097043:Interleukin-22; C090140:Whn protein
keywords: DN; DP; Dex; Foxn1; HCT; SP; TBI; TEC; Thymic epithelial cells;; Thymus;; dexamethasone; double negative; double positive; hematopoietic cell transplantation; single positive; thymic epithelial cell.; total body irradiation