title: Radiolabeling of new generation magnetic poly(HEMA-MAPA) nanoparticles with (131) I and preliminary investigation of its radiopharmaceutical potential using albino Wistar rats.
abstract: In this study, N-methacryloyl-l-phenylalanine (MAPA) containing poly(2-hydroxyethylmethacrylate) (HEMA)-based magnetic poly(HEMA-MAPA) nanobeads [mag-poly(HEMA-MAPA)] were radiolabeled with (131) I [(131) I-mag-poly(HEMA-MAPA)], and the radiopharmaceutical potential of (131) I-mag-poly(HEMA-MAPA) was investigated. Quality control studies were carried out by radiochromatographic method to be sure that (131) I binded to mag-poly(HEMA-MAPA) efficiently. In this sense, binding yield of (131) I-mag-poly(HEMA-MAPA) was found to be about 95-100%. In addition to this, optimum radiodination conditions for (131) I-mag-poly(HEMA-MAPA) were determined by thin-layer radiochromatography studies. In addition to thin-layer radiochromatography studies, lipophilicity (partition coefficient) and stability studies for (131) I-mag-poly(HEMA-MAPA) were realized. It was determined that lipophilicities of mag-poly(HEMA-MAPA) and (131) I-mag-poly(HEMA-MAPA) were 0.12 ± 0.01 and 1.79 ± 0.76 according to ACD/logP algorithm program, respectively. Stability of the radiolabeled compound was investigated in time intervals given as 0, 30, 60, 180, and 1440 min. It was found that (131) I-mag-poly(HEMA-MAPA) existed as a stable complex in rat serum within 60 min. After that, biodistribution and scintigraphy studies were carried out by using albino Wistar rats. It was determined that the most important (131) I activity uptake was observed in the breast, the ovary, and the pancreas. Scintigraphy studies well supported biodistribution results.
authors: Department of Chemistry, Faculty of Art and Science, Celal Bayar University, 45030, Manisa, Turkey.
authors: Uğur
authors: U
authors: Avcıbaşı
authors: Hasan
authors: H
authors: Demiroğlu
authors: Melis
authors: M
authors: Ediz
authors: Hilmi Arkut
authors: HA
authors: Akalın
authors: Emir
authors: E
authors: Özçalışkan
authors: Hilal
authors: H
authors: Şenay
authors: Ceren
authors: C
authors: Türkcan
authors: Yeşim
authors: Y
authors: Özcan
authors: Sinan
authors: S
authors: Akgöl
authors: Nesibe
authors: N
authors: Avcıbaşı
mesh_terms: D000417:Albinism; D000818:Animals; D007457:Iodine Radioisotopes / Q000737:chemistry*; D007553:Isotope Labeling; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000737:chemistry; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D019275:Radiopharmaceuticals / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
chemical_list: D007457:Iodine Radioisotopes; D058185:Magnetite Nanoparticles; D019275:Radiopharmaceuticals; D011102:Polyhydroxyethyl Methacrylate; D010649:Phenylalanine
keywords: 131I; drug carrier systems; nanobeads; poly(HEMA); poly(HEMA-MAPA)