title: C7: a CpG oligodeoxynucleotide that induces protective immune response against megalocytivirus in Japanese flounder (Paralichthys olivaceus) via Toll-like receptor 9-mediated signaling pathway.
abstract: Megalocytivirus is the causative agent of severe disease outbreaks in farmed fish. Currently there is no effective control against megalocytivirus in aquaculture. Synthetic oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs are known to possess marked immunostimulatory properties. In this study, we investigated the potentials of ten CpG ODNs as antiviral agents in a model of Japanese flounder (Paralichthys olivaceus). We found that, when administered into flounder, three of the ten CpG ODNs inhibited viral replication in kidney, spleen, and liver. ODN C7, which exhibited the strongest inhibitory activity, was able to promote proliferation of peripheral blood leukocytes and enhance activation of head kidney mononuclear adherent phagocytes. When the expression of toll-like receptor 9 (TLR9) was knocked down in vivo by small interfering RNA, C7-mediated immune response and antiviral activity were significantly blocked. Moreover, when C7 was co-administered with pCN86, a DNA vaccine against megalocytivirus, a significant increase in vaccine-induced protection was observed compared to administration with pCN86 alone. Further analysis showed that compared to fish immunized with pCN86, fish immunized with pCN86 plus C7 exhibited significantly upregulated expression of a wide range of genes involved in innate and adaptive immunity. Taken together, these results indicate that ODN C7 activates TLR9-mediated immune response and possesses antiviral and adjuvant potentials that may be exploited for the control of megalocytivirus infection in farmed flounder.
authors: Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China.
authors: Zhi-xia
authors: ZX
authors: Zhou
authors: Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors: Jian
authors: J
authors: Zhang
authors: Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China. Electronic address: lsun@qdio.ac.cn.
authors: Li
authors: L
authors: Sun
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage; D002478:Cells, Cultured; D004266:DNA Virus Infections / Q000235:genetics / Q000276:immunology* / Q000628:therapy*; D005432:Flounder / Q000276:immunology*; D060226:Head Kidney / Q000276:immunology*; D007109:Immunity / Q000235:genetics; D007497:Iridoviridae / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D012157:Mononuclear Phagocyte System / Q000276:immunology*; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D051217:Toll-Like Receptor 9 / Q000235:genetics / Q000378:metabolism*; D019444:Vaccines, DNA; D014765:Viral Vaccines; D014779:Virus Replication / Q000235:genetics
chemical_list: D000276:Adjuvants, Immunologic; D000998:Antiviral Agents; C408982:CPG-oligonucleotide; D009838:Oligodeoxyribonucleotides; D034741:RNA, Small Interfering; D051217:Toll-Like Receptor 9; D019444:Vaccines, DNA; D014765:Viral Vaccines
keywords: Adjuvant; CpG ODN; Immunostimulant; Megalocytivirus; Paralichthys olivaceus