title: Morphology, ultrastructure and molecular characterisation of Spiroxys japonica Morishita, 1926 (Spirurida: Gnathostomatidae) from Pelophylax nigromaculatus (Hallowell) (Amphibia: Ranidae).
abstract: Gnathostomatid nematodes identified morphologically as Spiroxys japonica Morishita, 1926 were collected from the dark-spotted frog Pelophylax nigromaculatus (Hallowell) (Amphibia: Ranidae) in China. Light and scanning electron microscopy were used to study the morphology of this species in detail. Previously unreported morphological features are revealed and others corrected. In addition, adult nematodes of S. japonica collected from P. nigromaculatus and Spiroxys hanzaki Hasegawa, Miyata & Doi, 1998 collected from Andrias japonicus (Temminck) (Caudata: Cryptobranchidae) in China and Japan, respectively, and the third-stage larva of S. japonica collected from Lithobates catesbeianus (Shaw) (Anura: Ranidae) in Japan, were characterised using molecular methods by sequencing and analysing ribosomal [large ribosomal DNA (18S) and internal transcribed space] and mitochondrial [cytochrome c oxidase subunit 1] target regions, respectively. The new morphological and genetic data contributes to a more accurate diagnosis of this hitherto little known nematode genus.
authors: Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Science, Hebei Normal University, 20 East Road of 2nd South Ring, Yuhua District, 050024, Shijiazhuang, Hebei Province, People's Republic of China, liangliangex369@126.com.
authors: Liang
authors: L
authors: Li
authors: Hideo
authors: H
authors: Hasegawa
authors: Vicente
authors: V
authors: Roca
authors: Zhen
authors: Z
authors: Xu
authors: Yan-Ning
authors: YN
authors: Guo
authors: Akiko
authors: A
authors: Sato
authors: Lu-Ping
authors: LP
authors: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China; D018509:DNA, Helminth / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005260:Female; D007564:Japan; D008297:Male; D008969:Molecular Sequence Data; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D011898:Ranidae / Q000469:parasitology*; D017174:Spirurida / Q000145:classification / Q000235:genetics* / Q000648:ultrastructure*; D014562:Caudata / Q000469:parasitology
chemical_list: D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S