title: Differences of bone healing in metaphyseal defect fractures between osteoporotic and physiological bone in rats.
abstract: Discrepancies in bone healing between osteoporotic and non-osteoporotic bone remain uncertain. The focus of the current work is to evaluate potential healing discrepancies in a metaphyseal defect model in rat femora. Female Sprague-Dawley rats were either ovariectomized (OVX, n=14) and combined with a calcium-, phosphorus- and vitamin D3-, soy- and phytoestrogen-free diet or received SHAM operation with standard diet rat (SHAM, n=14). Three months post-ovariectomy, DEXA measurement showed a reduction of bone mineral density reflecting an osteoporotic bone status in OVX rats. Rats then underwent a 3 mm wedge-shaped osteotomy at the distal metaphyseal area of the left femur stabilized with a T-shaped mini-plate and allowed to heal for 6 weeks. Biomechanical competence by means of a non-destructive three-point bending test showed significant lower flexural rigidity in the OVX rats at 3 mm lever span compared to SHAM animals (p=0.048) but no differences at 10 mm lever span. Microcomputer tomography (μCT) showed bridging cortices and consolidation of the defect in both groups, however, no measurable differences were found in either total ossified tissue or vascular volume fraction. Furthermore, histology showed healing discrepancies that were characterized by cartilaginous remnant and more unmineralized tissue presence in the OVX rats compared to more mature consolidation appearance in the SHAM group. In summary, bone defect healing in metaphyseal bone slightly differs between osteoporotic and non-osteoporotic bone in the current 3 mm defect model in both 3mm lever span biomechanical testing and histology.
authors: Department of Trauma Surgery, University Hospital of Giessen-Marburg, Giessen, Germany; Laboratory of Experimental Trauma Surgery, Justus-Liebig University, Giessen, Germany.
authors: Ulrich
authors: U
authors: Thormann
authors: Laboratory of Experimental Trauma Surgery, Justus-Liebig University, Giessen, Germany.
authors: Thaqif
authors: T
authors: El Khawassna
authors: Laboratory of Experimental Trauma Surgery, Justus-Liebig University, Giessen, Germany.
authors: Seemun
authors: S
authors: Ray
authors: Institute of Orthopedic Research and Biomechanics, Centre of Musculoskeletal Research, University of Ulm, Ulm, Germany.
authors: Lutz
authors: L
authors: Duerselen
authors: Department of Radiology, University Hospital of Giessen-Marburg, Giessen, Germany.
authors: Marian
authors: M
authors: Kampschulte
authors: Laboratory of Experimental Trauma Surgery, Justus-Liebig University, Giessen, Germany.
authors: Katrin
authors: K
authors: Lips
authors: Department of Radiology, University Hospital of Giessen-Marburg, Giessen, Germany.
authors: Helena
authors: H
authors: von Dewitz
authors: Institute of Materials Science, Technische Universität Dresden, Dresden, Germany.
authors: Sascha
authors: S
authors: Heinemann
authors: Department of Trauma Surgery, University Hospital of Giessen-Marburg, Giessen, Germany; Laboratory of Experimental Trauma Surgery, Justus-Liebig University, Giessen, Germany.
authors: Christian
authors: C
authors: Heiss
authors: Department of Trauma Surgery, University Hospital of Giessen-Marburg, Giessen, Germany.
authors: Gabor
authors: G
authors: Szalay
authors: Department of Diagnostic and Interventional Radiology, BG Trauma Hospital Frankfurt/Main, Germany.
authors: Alexander C
authors: AC
authors: Langheinrich
authors: Institute of Orthopedic Research and Biomechanics, Centre of Musculoskeletal Research, University of Ulm, Ulm, Germany.
authors: Anita
authors: A
authors: Ignatius
authors: Department of Trauma Surgery, University Hospital of Giessen-Marburg, Giessen, Germany; Laboratory of Experimental Trauma Surgery, Justus-Liebig University, Giessen, Germany.
authors: Reinhard
authors: R
authors: Schnettler
authors: Department of Trauma Surgery, University Hospital of Giessen-Marburg, Giessen, Germany; Laboratory of Experimental Trauma Surgery, Justus-Liebig University, Giessen, Germany. Electronic address: volker.alt@chiru.med.uni-giessen.de.
authors: Volker
authors: V
authors: Alt
mesh_terms: D000818:Animals; D015519:Bone Density; D002118:Calcium / Q000172:deficiency; D002762:Cholecalciferol / Q000172:deficiency; D004195:Disease Models, Animal; D004872:Ergocalciferols / Q000172:deficiency; D005260:Female; D005264:Femoral Fractures / Q000473:pathology*; D017102:Fracture Healing; D010024:Osteoporosis / Q000209:etiology / Q000473:pathology*; D058866:Osteoporotic Fractures / Q000473:pathology*; D010052:Ovariectomy / Q000009:adverse effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014808:Vitamin D Deficiency / Q000150:complications / Q000473:pathology
chemical_list: D004872:Ergocalciferols; D002762:Cholecalciferol; D002118:Calcium
keywords: Bone healing; Fracture; Metaphysis; Osteoporosis; Osteotomy