title: Upregulation of the long non-coding RNA PlncRNA-1 promotes esophageal squamous carcinoma cell proliferation and correlates with advanced clinical stage.
abstract: BACKGROUND
  Recent studies revealed that long noncoding RNAs (lncRNAs) play critical regulatory roles in cancer biology. PlncRNA-1 is one of lncRNAs that is associated with cell apoptosis and proliferation of prostate cancer.
  
  
  OBJECTIVE
  This study aimed to assess the potential role of PlncRNA-1 in the pathogenesis of esophageal squamous cell carcinoma (ESCC).
  
  
  METHODS
  Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression level of PlncRNA-1 in 73 pairs of ESCC and their matched normal tissues. The correlation of PlncRNA-1 with clinicopathological features and clinical stages was also analyzed. Cancer cell proliferation and apoptosis were assessed following knock-down of PlncRNA-1 by MTT, colony formation assay, and flow cytometry.
  
  
  RESULTS
  The expression of PlncRNA-1 was significantly higher in human ESCC compared with the adjacent noncancerous tissues (69.8 %, p < 0.05), and the high level of PlncRNA-1 expression was significantly correlated with advanced clinical stage (p < 0.01) and lymph node metastasis (p < 0.05). Furthermore, knockdown of PlncRNA-1 reduced cell proliferation and increased the apoptosis in vitro.
  
  
  CONCLUSIONS
  PlncRNA-1 plays an important role in ESCC cell proliferation. Overexpression of PlncRNA-1 is correlated with advanced tumor stage and lymph node metastasis, and may serve as a potential prognostic marker and therapeutic target for ESCC.
authors: Department of Surgical Oncology, Affiliated Nanjing Hospital of Nanjing Medical University, Nanjing, Jiangsu, China, ye-lucky@163.com.
authors: Chun-Mei
authors: CM
authors: Wang
authors: Qing-Quan
authors: QQ
authors: Wu
authors: Su-Qing
authors: SQ
authors: Li
authors: Fang-Jun
authors: FJ
authors: Chen
authors: Lei
authors: L
authors: Tuo
authors: Hai-Wei
authors: HW
authors: Xie
authors: Yu-Suo
authors: YS
authors: Tong
authors: Lv
authors: L
authors: Ji
authors: Guo-Zhi
authors: GZ
authors: Zhou
authors: Gang
authors: G
authors: Cao
authors: Ming
authors: M
authors: Wu
authors: Jin
authors: J
authors: Lv
authors: Wei-Hong
authors: WH
authors: Shi
authors: Xiu-Feng
authors: XF
authors: Cao
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D062085:RNA, Long Noncoding / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C587820:PlncRNA-1, human; D062085:RNA, Long Noncoding