title: Aptamer RA36 inhibits of human, rabbit, and rat plasma coagulation activated with thrombin or snake venom coagulases.
abstract: RA36 DNA aptamer is a direct anticoagulant prolonging clotting time of human, rabbit, and rat plasma in the thrombin time test. Anticoagulant activity of RA36 is lower than that of recombinant hirudin. During inhibition of human plasma clotting activated with echitox (coagulase from Echis multisquamatus venom), the aptamer presumably binds to meisothrombin exosite I. The sensitivity of human plasma to the aptamer 5-fold surpasses that of rat plasma. Analysis of RA36 binding to coagulase of Agkistrodon halys venom (ancistron) is required for proving the effect of aptamer on polymerization of human fibrinogen.
authors: Hematology Research Center, Ministry of Health and Social Development of the Russian Federation; APTO-PHARM, Moscow Region; Chemical Faculty, Faculty of Bioengineering and Bioinformatics, M. V. Lomonosov Moscow State University; Institute of Gene Biology, Russian Academy of Sciences, Moscow. nndrozd@mail.ru.
authors: E Yu
authors: EY
authors: Savchik
authors: T B
authors: TB
authors: Kalinina
authors: N N
authors: NN
authors: Drozd
authors: V A
authors: VA
authors: Makarov
authors: E G
authors: EG
authors: Zav'yalova
authors: E N
authors: EN
authors: Lapsheva
authors: N N
authors: NN
authors: Mudrik
authors: A V
authors: AV
authors: Babij
authors: G V
authors: GV
authors: Pavlova
authors: A V
authors: AV
authors: Golovin
authors: A M
authors: AM
authors: Kopylov
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D052157:Aptamers, Nucleotide / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects*; D003030:Coagulase / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D006801:Humans; D011817:Rabbits; D051381:Rats; D030162:Reptilian Proteins / Q000494:pharmacology*; D013917:Thrombin / Q000502:physiology*; D014757:Viper Venoms / Q000201:enzymology
chemical_list: D000925:Anticoagulants; D052157:Aptamers, Nucleotide; D003030:Coagulase; D030162:Reptilian Proteins; D014757:Viper Venoms; D013917:Thrombin