title: A promoter that drives gene expression preferentially in male transgenic rats.
abstract: Gender-preferential gene expression is a widespread phenomenon in humans. It is important to study how gender differences influence the pathogenesis of various diseases and response to specific drugs. The aim of this study is to determine if the mouse albumin enhancer/promoter may serve as the promoter to introduce gender-preferential gene expression in transgenic animals. We created four independent transgenic rat lines in which the human C-reactive protein transgene was under the control of mouse albumin enhancer/promoter. Quantitative real time RT-PCR analysis showed that transgene expression in the liver of male rats was significantly higher than transgene expression in the female rats (P < 0.05).There was a 5.3-fold (male/female) difference in line-519, and a 12.2-fold (male/female) difference in line-488. Enzyme-linked immunosorbent assay showed that the serum of male transgenic rats had a 13- to 679-fold difference at the protein level on transgene production compared with female transgenic rats. The male-to-female difference in gene expression was 10- to 17-fold in the liver of transgenic rats. Orchiectomy dramatically reduced protein production from the transgene in the liver. Testosterone administration into female rats did not increase the transgene expression, but estrogen administration into the male rats reduced transgene expression. This study provides a valuable tool for investigating the pathological roles of genes that are expressed in a gender-preferential manner in human disease.
authors: Department of Obstetrics and Gynecology, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China.
authors: Qiling
authors: Q
authors: Li
authors: Yamin
authors: Y
authors: Ma
authors: Wenzhi
authors: W
authors: Li
authors: Wei
authors: W
authors: Xu
authors: Li
authors: L
authors: Ma
authors: Guoxing
authors: G
authors: Fu
authors: Xiaohua
authors: X
authors: Tian
authors: Yueling
authors: Y
authors: Wang
authors: Xu
authors: X
authors: Li
authors: Tameka
authors: T
authors: Bythwood
authors: Jendai
authors: J
authors: Richards
authors: Mukaila A
authors: MA
authors: Akinbami
authors: Qing
authors: Q
authors: Song
mesh_terms: D000418:Albumins / Q000235:genetics*; D000818:Animals; D030801:Animals, Genetically Modified; D015153:Blotting, Western; D002097:C-Reactive Protein / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D004967:Estrogens / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D006801:Humans; D007279:Injections, Subcutaneous; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D009919:Orchiectomy; D010052:Ovariectomy; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D012737:Sex Factors; D013739:Testosterone / Q000008:administration & dosage / Q000494:pharmacology; D019076:Transgenes / Q000235:genetics*
chemical_list: D000418:Albumins; D017931:DNA Primers; D004967:Estrogens; D013739:Testosterone; D002097:C-Reactive Protein