title: Formation and dissolution of chitosan/pyrophosphate nanoparticles: is the ionic crosslinking of chitosan reversible?
abstract: Ionically crosslinked chitosan particles with submicron dimensions attract widespread interest as materials for controlled release. To this end, we have examined the formation and dissolution of nanoparticles prepared by crosslinking chitosan with pyrophosphate (PPi). The formation of these particles required a critical PPi concentration (which increased with the chitosan concentration), and their z-average hydrodynamic diameters could be predictably tuned from roughly 60 to 220 nm by varying the concentration of the parent chitosan solutions. Unlike the nanoparticles crosslinked with the commonly used tripolyphosphate (TPP), which coagulated and precipitated when TPP was in excess, the chitosan/PPi nanoparticles remained colloidally stable even at high PPi concentrations. Moreover, the analysis of their dissolution revealed hysteresis in the particle formation/dissolution cycle, where portions of the crosslinked chitosan/PPi complexes remained stably intact at PPi concentrations below those required for their formation. This irreversible behavior was surmised to reflect the cooperativity of chitosan/PPi binding and was qualitatively modeled using the Bragg-Williams theory.
authors: Dept. of Chemical and Environmental Engineering, University of Toledo, Toledo, OH 43606, USA.
authors: Yuhang
authors: Y
authors: Cai
authors: Dept. of Chemical and Environmental Engineering, University of Toledo, Toledo, OH 43606, USA; School of Green Chemistry and Engineering, University of Toledo, Toledo, OH 43606, USA. Electronic address: yakov.lapitsky@utoledo.edu.
authors: Yakov
authors: Y
authors: Lapitsky
mesh_terms: D048271:Chitosan / Q000138:chemical synthesis* / Q000737:chemistry; D003102:Colloids / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry*; D011756:Diphosphates / Q000138:chemical synthesis / Q000737:chemistry*; D057446:Hydrodynamics; D007477:Ions; D007700:Kinetics; D008027:Light; D008962:Models, Theoretical; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011122:Polyphosphates / Q000138:chemical synthesis / Q000737:chemistry; D012542:Scattering, Radiation; D012995:Solubility; D012996:Solutions
chemical_list: D003102:Colloids; D003432:Cross-Linking Reagents; D011756:Diphosphates; D007477:Ions; D011122:Polyphosphates; D012996:Solutions; C107241:diphosphoric acid; D048271:Chitosan; C005692:triphosphoric acid
keywords: Chitosan; Hysteresis; Ionic crosslinking; Nanoparticles; Pyrophosphate