title: Repeated exposure to propofol impairs spatial learning, inhibits LTP and reduces CaMKIIα in young rats.
abstract: Propofol is one of the most widely used intravenous anesthetics. We investigated the effects of propofol injected during development on synaptic plasticity and long-term spatial learning and memory in young rats. Propofol (75 mg/kg) was administered to 7-day-old rats either as a single dose or in 7 doses at 24-h intervals. At postnatal day 28, spatial learning in the Morris water maze and long-term potentiation (LTP) in the CA1 region of the hippocampus were significantly reduced in the rats that had received 7 doses of propofol. This treatment also significantly decreased the expression of CaMKIIα and pCaMKIIα in the hippocampus, and reduced the pCaMKIIα/CaMKIIα ratio, as measured by immunochemistry and Western blotting. We conclude that repeated exposure to propofol impairs learning and memory in the developing rat brain, and this finding may be associated with down-regulation of CaMKIIα and pCaMKIIα.
authors: Department of Anesthesiology, First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China.
authors: Jin
authors: J
authors: Gao
authors: Department of Anesthesiology, First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China; Department of Anesthesiology, Chongqing Hospital of Traditional Chinese Medical, Chongqing, PR China.
authors: Shujun
authors: S
authors: Peng
authors: Department of Anesthesiology, First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China.
authors: Shiqi
authors: S
authors: Xiang
authors: Department of Anesthesiology, First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China.
authors: Jiayu
authors: J
authors: Huang
authors: Department of Anesthesiology, First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China. Electronic address: mazuichen@163.com.
authors: Ping
authors: P
authors: Chen
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000037:antagonists & inhibitors*; D005260:Female; D007858:Learning / Q000187:drug effects*; D017774:Long-Term Potentiation / Q000187:drug effects*; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D008568:Memory / Q000187:drug effects; D009949:Orientation / Q000187:drug effects; D010766:Phosphorylation; D015742:Propofol / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D013028:Space Perception / Q000187:drug effects*
chemical_list: D018686:Anesthetics, Intravenous; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D015742:Propofol
keywords: Ca(2+)/calmodulin-dependent protein kinase type 2; Learning; Long term potentiation; Memory; Propofol; Synaptic plasticity