title: Method development and validation for simultaneous determination of lumefantrine and its major metabolite, desbutyl lumefantrine in human plasma using RP-HPLC/UV detection.
abstract: A simple, specific, precise and rapid RP-HPLC-UV method was developed for simultaneous determination of lumefantrine and its metabolite desbutyl lumefantrine in human plasma. Experimental parameters were optimized and the method was validated according to standard guidelines. The method showed adequate separation for lumefantrine and desbutyl lumefantrine and best resolution was achieved with Supelco Discovery HS C18 RP (150mm×4.6mm, 5μm) column using acetonitrile and 0.05% trifluroacetic acid (70:30, v/v) as a mobile phase pumped at a flow rate of 1.0ml/min and wavelength of 335nm. The method was linear over the concentration range of 10-12,000ng/ml. The lower limit of detection (LLOD) and lower limit of quantification (LLOQ) for lumefantrine were 10.0 and 18.0ng/ml, while for desbutyl lumefantrine were 7.5 and 15.0ng/ml, respectively. The proposed method was efficiently applied for determination of lumefantrine and desbutyl lumefantrine concentrations in plasma samples for pharmacokinetic studies.
authors: Department of Pharmacy, University of Peshawar, Peshawar 25120, Pakistan.
authors: Fazli
authors: F
authors: Khuda
authors: Department of Pharmacy, University of Peshawar, Peshawar 25120, Pakistan. Electronic address: iqbal@upesh.edu.pk.
authors: Zafar
authors: Z
authors: Iqbal
authors: Department of Pharmacy, University of Peshawar, Peshawar 25120, Pakistan.
authors: Yasar
authors: Y
authors: Shah
authors: Department of Pharmacy, University of Peshawar, Peshawar 25120, Pakistan.
authors: Lateef
authors: L
authors: Ahmmad
authors: Department of Pharmacy, University of Peshawar, Peshawar 25120, Pakistan.
authors: Fazli
authors: F
authors: Nasir
authors: Department of Pharmacy, University of Peshawar, Peshawar 25120, Pakistan.
authors: Amir Zada
authors: AZ
authors: Khan
authors: Department of Pharmacy, University of Peshawar, Peshawar 25120, Pakistan.
authors: Amanullah
authors: Department of Pharmacy, University of Peshawar, Peshawar 25120, Pakistan.
authors: Naila
authors: N
authors: Shahbaz
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D004355:Drug Stability; D004983:Ethanolamines / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D005449:Fluorenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D000078102:Lumefantrine; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004983:Ethanolamines; D005449:Fluorenes; C535063:desbutyllumefantrine; D000078102:Lumefantrine
keywords: Desbutyl lumefantrine; Lumefantrine; RP-HPLC/UV; Validation