title: Purification, crystallization and preliminary X-ray analysis of IMP-18, a class B carbapenemase from Pseudomonas aeruginosa.
abstract: Class B β-lactamases are known as metallo-β-lactamases (MBLs) and they hydrolyze most β-lactams, including carbapenems. IMP-18, an MBL cloned from Pseudomonas aeruginosa, was overexpressed, purified and crystallized by vapour diffusion for X-ray crystallographic analysis. Preliminary X-ray analysis showed that the crystal diffracted to 2.4 Å resolution and belonged to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 120.77, c = 96.54 Å, α = β = γ = 90°, suggesting the presence of two molecules in the asymmetric unit.
authors: Department of Material and Biological Chemistry, Graduate School of Science and Engineering, Yamagata University, 1-4-12 Kojirakawa, Yamagata 990-8560, Japan.
authors: Takamitsu
authors: T
authors: Furuyama
authors: Yoshikazu
authors: Y
authors: Ishii
authors: Norimasa
authors: N
authors: Ohya
authors: Kazuhiro
authors: K
authors: Tateda
authors: Nancy D
authors: ND
authors: Hanson
authors: Akiko
authors: A
authors: Shimizu-Ibuka
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001618:beta-Lactamases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D001618:beta-Lactamases; C527279:beta-lactamase IMP-18, Pseudomonas aeruginosa
keywords: IMP-18; Pseudomonas aeruginosa; carbapenemases; class B β-lactamases