title: Cytotoxicity study on SH‑SY5Y cells cultured at high glucose levels and treated with bupivacaine.
abstract: The aim of this study was to investigate susceptibility to the neurotoxicity of local anesthetic agents in a model of gestational diabetes mellitus (GDM). SH-SY5Y cells were cultured at different concentrations of glucose and subsequently treated with 1 mmol/l bupivacaine for 6 h. Reactive oxygen species (ROS) production and apoptosis were assessed using flow cytometry in each group of cells. The MTT method was utilized to detect cell survival, and western blot analysis was used to examine changes in 78 kDa glucose‑regulated protein (GRP78) levels in neuronal cells. In all groups, levels of ROS production, cell survival and GRP78 expression were significantly different (P<0.01) following the addition of various concentrations of glucose and bupivacaine, as well as for the interaction between different concentrations of the anesthetic agents, demonstrating a statistically significant difference. In conclusion, the susceptibility of SH-SY5Y cells to the neurotoxicity of local anesthetic agents was enhanced in a model of GDM.
authors: Department of Anesthesia, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, P.R. China.
authors: Yawen
authors: Y
authors: Li
authors: Department of Anesthesia, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, P.R. China.
authors: Shiyuan
authors: S
authors: Xu
authors: Department of Anesthesia, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, P.R. China.
authors: Qingguo
authors: Q
authors: Zhang
authors: Department of Anesthesia, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, P.R. China.
authors: Le
authors: L
authors: Li
authors: Department of Anesthesia, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, P.R. China.
authors: Luying
authors: L
authors: Lai
authors: Department of Anesthesia, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, P.R. China.
authors: Ting
authors: T
authors: Zheng
authors: Department of Anesthesia, Shenzhen Maternal and Child Health Hospital, Southern Medical University, Shenzhen, Guangdong 510282, P.R. China.
authors: Jiaoling
authors: J
authors: Su
authors: Department of Anesthesia, Shenzhen Maternal and Child Health Hospital, Southern Medical University, Shenzhen, Guangdong 510282, P.R. China.
authors: Naimei
authors: N
authors: Yang
authors: Department of Anesthesia, Shenzhen Maternal and Child Health Hospital, Southern Medical University, Shenzhen, Guangdong 510282, P.R. China.
authors: Yuantao
authors: Y
authors: Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002045:Bupivacaine / Q000008:administration & dosage*; D002470:Cell Survival / Q000187:drug effects; D016640:Diabetes, Gestational / Q000188:drug therapy* / Q000473:pathology; D000091342:Endoplasmic Reticulum Chaperone BiP; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D017382:Reactive Oxygen Species; D005947:Glucose; D002045:Bupivacaine