title: Proinflammatory effects and oxidative stress within human bronchial epithelial cells exposed to atmospheric particulate matter (PM(2.5) and PM(>2.5)) collected from Cotonou, Benin.
abstract: After particulate matter (PM) collection in Cotonou (Benin), a complete physicochemical characterization of PM2.5 and PM>2.5 was led. Then, their adverse health effects were evaluated by using in vitro culture of human lung cells. BEAS-2B (bronchial epithelial cells) were intoxicated during short-term exposure at increasing PM concentrations (1.5-96 μg/cm(2)) to determine global cytotoxicity. Hence, cells were exposed to 3 and 12 μg/cm(2) to investigate the potential biological imbalance generated by PM toxicity. Our findings showed the ability of both PM to induce oxidative stress and to cause inflammatory cytokines/chemokines gene expression and secretion. Furthermore, PM were able to induce gene expression of enzymes involved in the xenobiotic metabolism pathway. Strong correlations between gene expression of metabolizing enzymes, proinflammatory responses and cell cycle alteration were found, as well as between proinflammatory responses and cell viability. Stress oxidant parameters were highly correlated with expression and protein secretion of inflammatory mediators.
authors: Université Lille Nord de France, Lille, France; Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV) EA 4492, Maison de la Recherche en Environnement Industriel 2, Université du Littoral Côte d'Opale, 189A Avenue Maurice Schumann, 59140 Dunkerque, France; Laboratoire de Biochimie et Biologie Moléculaire, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, 04 BP 0320, Cotonou, Benin.
authors: Boris Fresnel
authors: BF
authors: Cachon
authors: Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV) EA 4492, Maison de la Recherche en Environnement Industriel 2, Université du Littoral Côte d'Opale, 189A Avenue Maurice Schumann, 59140 Dunkerque, France; UPSP-EGEAL, Institut Polytechnique LaSalle Beauvais, 60026 Beauvais Cedex, France.
authors: Stéphane
authors: S
authors: Firmin
authors: Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV) EA 4492, Maison de la Recherche en Environnement Industriel 2, Université du Littoral Côte d'Opale, 189A Avenue Maurice Schumann, 59140 Dunkerque, France. Electronic address: verdin@univ-littoral.fr.
authors: Anthony
authors: A
authors: Verdin
authors: Laboratoire de Biochimie et Biologie Moléculaire, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, 04 BP 0320, Cotonou, Benin.
authors: Lucie
authors: L
authors: Ayi-Fanou
authors: Université Lille Nord de France, Lille, France; Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV) EA 4492, Maison de la Recherche en Environnement Industriel 2, Université du Littoral Côte d'Opale, 189A Avenue Maurice Schumann, 59140 Dunkerque, France.
authors: Sylvain
authors: S
authors: Billet
authors: Université Lille Nord de France, Lille, France; Centre Commun de Mesures, Maison de la Recherche en Environnement Industriel 1, Université du Littoral Côte d'Opale, 145 Avenue Maurice Schumann, 59140 Dunkerque, France.
authors: Fabrice
authors: F
authors: Cazier
authors: Université Lille Nord de France, Lille, France; Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV) EA 4492, Maison de la Recherche en Environnement Industriel 2, Université du Littoral Côte d'Opale, 189A Avenue Maurice Schumann, 59140 Dunkerque, France.
authors: Perrine J
authors: PJ
authors: Martin
authors: Université Lille Nord de France, Lille, France.
authors: Faustin
authors: F
authors: Aissi
authors: Université Lille Nord de France, Lille, France; Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV) EA 4492, Maison de la Recherche en Environnement Industriel 2, Université du Littoral Côte d'Opale, 189A Avenue Maurice Schumann, 59140 Dunkerque, France.
authors: Dominique
authors: D
authors: Courcot
authors: Laboratoire de Biochimie et Biologie Moléculaire, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, 04 BP 0320, Cotonou, Benin.
authors: Ambaliou
authors: A
authors: Sanni
authors: Université Lille Nord de France, Lille, France; Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV) EA 4492, Maison de la Recherche en Environnement Industriel 2, Université du Littoral Côte d'Opale, 189A Avenue Maurice Schumann, 59140 Dunkerque, France.
authors: Pirouz
authors: P
authors: Shirali
mesh_terms: D000393:Air Pollutants / Q000378:metabolism / Q000633:toxicity*; D001272:Atmosphere; D001541:Benin; D002460:Cell Line; D002470:Cell Survival; D004784:Environmental Monitoring; D004847:Epithelial Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000187:drug effects; D018384:Oxidative Stress; D052638:Particulate Matter / Q000378:metabolism / Q000633:toxicity*
chemical_list: D000393:Air Pollutants; D052638:Particulate Matter
keywords: Benin; Cytotoxicity; Inflammatory response; Oxidative stress; Particulate matter