title: Combining peptide and DNA for protein assay: CRIP1 detection for breast cancer staging.
abstract: In this work, a novel method for a protein assay is proposed which uses the specific protein-binding peptide of the target protein and sequence-specific DNA to interact with the target as the capture and detection probe, respectively. Meanwhile, since the DNA sequence can be coupled with gold nanoparticles to amplify the signal readout, a sensitive and easily operated method for protein assay is developed. We have also employed a transcription factor named as cysteine-rich intestinal protein 1 (CRIP1), which has been identified as an ideal biomarker for staging of breast cancer, as the model protein for this study. With the proposed method, CRIP1 can be determined in a linear range from 1.25 to 10.13 ng/mL, with a detection limit of 1.25 ng/mL. Furthermore, the proposed method can be directly used to assay CRIP1 in tissue samples. Owing to its desirable sensitivity, excellent reproducibility, and high selectivity, the proposed method may hold great potential in clinical practice in the future.
authors: Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University , 210093 Nanjing, China.
authors: Haona
authors: H
authors: Xie
authors: Hao
authors: H
authors: Li
authors: Yue
authors: Y
authors: Huang
authors: Xiaoying
authors: X
authors: Wang
authors: Yongmei
authors: Y
authors: Yin
authors: Genxi
authors: G
authors: Li
mesh_terms: D001483:Base Sequence; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D002352:Carrier Proteins / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004566:Electrodes; D005260:Female; D006801:Humans; D060588:LIM Domain Proteins / Q000378:metabolism*; D057230:Limit of Detection; D009367:Neoplasm Staging / Q000379:methods*; D010455:Peptides / Q000378:metabolism*; D015203:Reproducibility of Results
chemical_list: C486181:CRIP1 protein, human; D002352:Carrier Proteins; D004277:DNA, Single-Stranded; D060588:LIM Domain Proteins; D010455:Peptides