title: Flavonoid mixture ameliorates increase in erythrocyte osmotic fragility and malondialdehyde concentration induced by Trypanosoma brucei brucei-infection in Wistar rats.
abstract: The experiment was performed with the aim of investigating the effect of a flavonoid mixture, Daflon® 500 mg (DF) on the erythrocyte fragility and lipoperoxidative changes, induced by Trypanosoma brucei brucei infection in Wistar rats. Fifty adult male rats randomly divided into five groups of 10 animals each were used. Rats in the control group were administered (1 mL/kg) distilled water only, while the other groups were infected with T. brucei brucei and treated with Daflon® 500 mg and/or Diminazene aceturate. At the end of 5 weeks, EDTA-blood samples and serum samples were collected from the rats, and were used to determine erythrocyte osmotic fragility (EOF) and serum malondialdehyde (MDA) concentration respectively. The results showed that EOF and MDA concentration significantly (P<0.05) increased in the infected untreated group when compared to the treatment groups. Treatment with Daflon® 500 mg and Diminazene aceturate significantly (P<0.05) reduced trypanosome-induced increases in EOF and lipoperoxidative changes, suggesting possible antioxidant properties of Daflon® 500 mg and its therapeutic value in trypanosomosis.
authors: Department of Veterinary Pharmacology and Toxicology, Ahmadu Bello University, Zaria, Nigeria. Electronic address: patriciakobo@yahoo.com.
authors: Patricia I
authors: PI
authors: Kobo
authors: Department of Veterinary Physiology, Ahmadu Bello University, Zaria, Nigeria.
authors: Joseph O
authors: JO
authors: Ayo
authors: Department of Veterinary Physiology, Ahmadu Bello University, Zaria, Nigeria.
authors: Tagang
authors: T
authors: Aluwong
authors: Department of Clinical Pharmacy and Therapeutics, Ahmadu Bello University, Zaria, Nigeria.
authors: Abdulkadir U
authors: AU
authors: Zezi
authors: College of Agriculture and Animal Science, Ahmadu Bello University, Mando-Kaduna, Nigeria.
authors: Victor
authors: V
authors: Maikai
authors: Department of Veterinary Pharmacology and Toxicology, Ahmadu Bello University, Zaria, Nigeria; Department of Veterinary Physiology and Pharmacology, University of Ilorin, Ilorin, Nigeria.
authors: Suleiman F
authors: SF
authors: Ambali
mesh_terms: D000818:Animals; D004133:Diminazene / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D004145:Diosmin / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D004912:Erythrocytes / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009996:Osmotic Fragility / Q000187:drug effects; D018512:Parasitemia / Q000188:drug therapy / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014346:Trypanosoma brucei brucei / Q000254:growth & development*; D014353:Trypanosomiasis, African / Q000188:drug therapy / Q000378:metabolism*
chemical_list: D008315:Malondialdehyde; D004145:Diosmin; C003915:diminazene aceturate; D004133:Diminazene
keywords: Erythrocyte osmotic fragility; Flavonoids; Lipoperoxidation; Trypanosoma brucei brucei