title: Method of cytogenetic assay of mouse oocytes.
abstract: We developed an original method of isolation and analysis of cytogenetic micropreparations of mouse oocytes including treatment with buffered hypotonic saline, paraformaldehyde fixation, and fluorescent staining. The method had several advantages, including high quality of sections and low labour intensity.
authors: V. V. Zakusov Research Institute of Pharmacology, Russian Academy of Medical Sciences, Moscow, Russia. kira-pligina@rambler.ru.
authors: K L
authors: KL
authors: Pligina
authors: A K
authors: AK
authors: Zhanataev
authors: Z V
authors: ZV
authors: Chaika
authors: A D
authors: AD
authors: Durnev
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D033481:Chromosomes, Mammalian / Q000378:metabolism; D020732:Cytogenetic Analysis; D005260:Female; D008540:Meiosis; D008677:Metaphase; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009865:Oocytes / Q000502:physiology*; D013480:Superovulation