title: Chondroprotective activity of a detoxicated traditional Chinese medicine (Fuzi) of Aconitum carmichaeli Debx against severe-stage osteoarthritis model induced by mono-iodoacetate.
abstract: BACKGROUND
  Fuzi is an effective but toxic traditional Chinese medicine (TCM) derived from Aconitum carmichaeli. In our previous study, detoxicated Fuzi (d-Fuzi) has been originally developed with no toxicity but significant efficacy. However, whether d-Fuzi can be used for therapy of osteoarthritis (OA), remain unknown.
  
  
  METHODS
  Severe OA model was established by intra-articular mono-iodoacetate (MIA) injection (1.25mg) into rats and orally treated with 2g/ml d-Fuzi at a dosage of 7 ml/kg body weight for 28 days. In vivo, the articular radiographic and histopathologic analyses were performed to qualitatively assess the chondroprotective effect of d-Fuzi, followed by quantitative measurements of bone density and Mankin scores. In vitro, such effect on chondrocyte viability after MIA attack was evaluated. Hybrid quadrupole time-of-flight mass spectrometry (QTOF-MS) was performed for chemical analysis of d-Fuzi.
  
  
  RESULTS
  d-Fuzi was demonstrated to possess chondroprotective activity on MIA-induced OA model by in vivo preventing the articular degeneration and the reducing of bone density and Mankin score, as well as by in vitro promoting the chondrocyte proliferation and inhibiting the MIA-induced chondrocyte damage. A total of 23 compounds were identified in d-Fuzi, most of which were deduced as the non-toxic derivatives of aconite alkaloids.
  
  
  CONCLUSIONS
  This is the first report regarding chondroprotective effect and chemical profile of d-Fuzi, originally revealing its great anti-OA potential and thereby providing a promising TCM candidate for OA therapy.
authors: Department of Orthopaedic Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310053, China; Institute of Orthopaedics and Traumatology, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors: Peijian
authors: P
authors: Tong
authors: Institute of Orthopaedics and Traumatology, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors: Shibing
authors: S
authors: Xu
authors: Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors: Gang
authors: G
authors: Cao
authors: College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors: Wangdong
authors: W
authors: Jin
authors: College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors: Yanwei
authors: Y
authors: Guo
authors: College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors: Yu
authors: Y
authors: Cheng
authors: Institute of Orthopaedics and Traumatology, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors: Hongting
authors: H
authors: Jin
authors: Institute of Orthopaedics and Traumatology, Zhejiang Chinese Medical University, Hangzhou 310053, China. Electronic address: letian.shan@hotmail.com.
authors: Letian
authors: L
authors: Shan
authors: Institute of Orthopaedics and Traumatology, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors: Luwei
authors: L
authors: Xiao
mesh_terms: D000818:Animals; D004224:Diterpenes; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D019807:Iodoacetic Acid / Q000633:toxicity*; D008297:Male; D010003:Osteoarthritis / Q000139:chemically induced* / Q000188:drug therapy*; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C575009:fuzi drug herbal; D019807:Iodoacetic Acid
keywords: Chondroprotective effect; Detoxicated Fuzi; Mono-iodoacetate; Osteoarthritis; Traditional Chinese medicine