title: Morphological and molecular identification of two strains of dermatophytes.
abstract: In this study, we used traditional morphological and molecular identification methods to preliminarily identify two strains of dermatophytes. The two strains were observed under the microscope. And then the dermatophytes were cultured on Sabouraud's dextrose agar (SDA). The 18S rRNA regions of the two dermatophyte strains were amplified by polymerase chain reaction (PCR), and the PCR products were sequenced and compared with GenBank data. BLAST tools and DNAMAN software were used to analyze the sequences. To further determine highly homologous sequences, a phylogenetic tree was constructed using the Neighbor-Joining method. The two strains of dermatophytes were identified by traditional morphological identification as Epidermophyton floccosum and Microsporum ferrugineum. The 18S rRNA sequence analyses showed high similarities to Cladosporium cladosporioides isolate C115LM-UFPR and Ascomycete sp. LB68A1A2. Epidermophyton and Cladosporium belong to dermatophyte, while Microsporum ferrugineum and Ascomycete belong to microsporum. The two novel strains of dermatophytes were therefore identified as Cladosporium cladosporioides isolate C115LM-UFPR (JN650537, Cladosporium) and Ascomycete sp. LB68A1A2 (AY770409, Ascomycete sp).
authors: College of Clinical Medicine, Hebei United University, Tangshan, 063000, China.
authors: Li
authors: L
authors: Chen
authors: Jitang College, Hebei United University, Tangshan, 063000, China.
authors: Guo-You
authors: GY
authors: Shi
authors: Department of Pathogeny Biology, College of Basic Medicine, Hebei United University, Tangshan, 063000, China.
authors: Mei-Mei
authors: MM
authors: Wang
authors: Department of Pharmacy, Pharmaceutical College, Hebei United University, Tangshan, 063000, China.
authors: Lin-Lin
authors: LL
authors: Zhao
authors: Department of Biotechnology, College of Life Sciences, Hebei United University, Tangshan, 063000, China.
authors: Yuan-Yong
authors: YY
authors: Huang
authors: College of Clinical Medicine, Hebei United University, Tangshan, 063000, China.
authors: Xiao-Lei
authors: XL
authors: Chen
authors: Department of Pathogeny Biology, College of Basic Medicine, Hebei United University, Tangshan, 063000, China.
authors: Li-Jie
authors: LJ
authors: Yuan
authors: Department of Pathogeny Biology, College of Basic Medicine, Hebei United University, Tangshan, 063000, China.
authors: Ya-Nan
authors: YN
authors: Xiong
authors: Department of Anatomy, College of Basic Medicine, Hebei United University, Tangshan, 063000, China.
authors: Dao-Wen
authors: DW
authors: Si
authors: Department of Pathogeny Biology, College of Basic Medicine, Hebei United University, Tangshan, 063000, China. zhulihua1972@163.com.
authors: Li-Hua
authors: LH
authors: Zhu
mesh_terms: D003883:Arthrodermataceae / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D025301:Hyphae / Q000166:cytology; D012331:RNA, Fungal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012867:Skin / Q000382:microbiology
chemical_list: D012331:RNA, Fungal; D012337:RNA, Ribosomal, 18S