title: Simultaneous immobilization of heparin and gentamicin on polypropylene textiles: a dual therapeutic activity.
abstract: The aim of this work was to prepare a nonwoven polypropylene (PP) textile functionalized with bioactive molecules in order to improve simultaneously anticoagulation and antibacterial properties. The immobilization of either heparin (anticoagulation agent) or gentamicin (aminoglycoside class antibiotic) alone has already been proven to be effective on PP nonwoven textiles. In this work, we managed to go further, by immobilizing both heparin and gentamicin at the same time on one unique textile. A successive immersion in different heparin and gentamicin bathes successfully led to a dual drug coated textile, as confirmed by several characterization techniques (Fourier transform infrared-attenuated total reflectance, X-ray photoelectron spectroscopy, and scanning electron microscopy). The immobilization times were varied in order to determine the best compromise between cytocompatibility, anticoagulant effect, and antimicrobial activity. Short immersion times in gentamicin solutions confer very good antimicrobial activity to the textile and avoid cytotoxicity, whereas long immersion times in heparin solution were necessary to observe a significant anticoagulant effect.
authors: Université Lille Nord de France, Lille, 59000, France; Unité des Matériaux et Transformations (UMET), CNRS-UMR 8207, Université Lille 1, Villeneuve d'Ascq, 59655, France.
authors: Stéphanie
authors: S
authors: Degoutin
authors: Maude
authors: M
authors: Jimenez
authors: Feng
authors: F
authors: Chai
authors: Thibaut
authors: T
authors: Pinalie
authors: Severine
authors: S
authors: Bellayer
authors: Marianne
authors: M
authors: Vandenbossche
authors: Christel
authors: C
authors: Neut
authors: Nicolas
authors: N
authors: Blanchemain
authors: Bernard
authors: B
authors: Martel
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D005260:Female; D005839:Gentamicins / Q000737:chemistry / Q000494:pharmacology; D006493:Heparin / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008297:Male; D008422:Materials Testing / Q000379:methods; D011126:Polypropylenes / Q000737:chemistry / Q000494:pharmacology; D013784:Textiles
chemical_list: D000900:Anti-Bacterial Agents; D000925:Anticoagulants; D005839:Gentamicins; D011126:Polypropylenes; D006493:Heparin
keywords: antibacterial; anticoagulant; cold plasma; cytocompatibility; multiple drug immobilization; polypropylene