title: Substrates with patterned extracellular matrix and subcellular stiffness gradients reveal local biomechanical responses.
abstract: A substrate fabrication process is developed to pattern both the extracellular matrix (ECM) and rigidity at sub-cellular spatial resolution. When growing cells on these substrates, it is found that cells respond locally in their cytoskeleton assembly. The presented method allows unique insight into the biological interpretation of mechanical signals, whereas photolithography-based fabrication is amenable to integration with complex microfabricated substructures.
authors: Department of Bioengineering, University of California Los Angeles, 420 Westwood Plaza 5121 Engineering V, Los Angeles, CA, 90095, USA.
authors: Peter
authors: P
authors: Tseng
authors: Dino
authors: D
authors: Di Carlo
mesh_terms: D016475:3T3 Cells; D020106:Acrylamide / Q000737:chemistry; D000199:Actins / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000295:instrumentation; D048430:Cell Shape / Q000502:physiology; D003470:Culture Media; D003599:Cytoskeleton / Q000502:physiology*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D055119:Elastic Modulus; D004548:Elasticity; D004853:Epoxy Resins / Q000737:chemistry; D005109:Extracellular Matrix / Q000737:chemistry*; D005347:Fibroblasts / Q000502:physiology; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D051379:Mice; D055616:Microtechnology; D024462:Myosin Type II / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D000199:Actins; D003470:Culture Media; D004129:Dimethylpolysiloxanes; D004853:Epoxy Resins; D006576:Heterocyclic Compounds, 4 or More Rings; D020106:Acrylamide; C472645:blebbistatin; C013830:baysilon; D024462:Myosin Type II
keywords: BioMEMS; biomechanics; cell control; mechanotransduction; stress fibers