title: Synergistic antiosteoporotic effect of Lepidium sativum and alendronate in glucocorticoid-induced osteoporosis in Wistar rats.
abstract: Alendronate belongs to a class of drugs called bisphosphonates. Bisphosphonates (BP) therapy is a vital option to reduce the risk of bone fracture in people who suffer from osteoporosis. Yet, bisphosphonate have displayed several side effects. Lepidium sativum (LS) seeds have been used in traditional folk medicine to heal fractured bones. However, there is a dearth of information on the impact of LS on bone metabolism especially in cases of glucocorticoids induced osteoporosis. Therefore, the aim of the study was to compare the biochemical bone markers and histological responses of LS alone (6 g of LS seeds in diet daily, n=8), ALD (alendronate, 70 mg/kg s.c.; n=8) alone, or LS and ALD combined in a rat model of glucocorticoid-induced osteoporosis (GIO) by injecting rats with methylprednisolone 3.5 mg/kg per day for 4 weeks. Serum calcium (Ca), albumin, phosphorus (P), bone-specific alkaline phosphatase (b-ALP), and tartrate-resistant acid phosphatase (TRAP) were measured 4 weeks after induction of GIO. GIO-group showed significantly increased serum TRAP and decreased b-ALP. GIO-group also showed significantly decreased serum P and unaltered Ca concentrations. Histological examination of GIO-group tibia bones indicated an osteoporotic change and a concomitant decrease in percentage of trabecular area or bone marrow area (PTB) in the proximal femoral epiphysis. Treatment with either LS and/or ALD ameliorated the above mentioned changes with variable degrees, with a net results of enhanced serum calcium, bone architecture, PTB, b-ALP and decreased TRAP in LS and LS+ALD groups compared to that of animals treated with alendronate alone. In conclusion, our findings present evidence supporting the potential benefits of LS in reducing the burden of GCs on bone health.
authors: Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia ; Department of Molecular Biology, Genetic Engineering and Biotechnology Institute, Sadat City University, Sadat City, Egypt.
authors: Mohamed F
authors: MF
authors: Elshal
authors: Abdulrahman L
authors: AL
authors: Almalki
authors: Hussein K
authors: HK
authors: Hussein
authors: Jalal A
authors: JA
authors: Khan
mesh_terms: D019386:Alendronate / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D015415:Biomarkers / Q000097:blood; D050071:Bone Density Conservation Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D002118:Calcium / Q000097:blood; D004195:Disease Models, Animal; D004357:Drug Synergism; D005260:Female; D005269:Femur; D005938:Glucocorticoids; D031220:Lepidium sativum; D010024:Osteoporosis / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D010744:Phosphoric Monoester Hydrolases / Q000097:blood; D010758:Phosphorus / Q000097:blood; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013977:Tibia
chemical_list: D015415:Biomarkers; D050071:Bone Density Conservation Agents; D005938:Glucocorticoids; D010936:Plant Extracts; D010758:Phosphorus; D010744:Phosphoric Monoester Hydrolases; D002118:Calcium; D019386:Alendronate
keywords: Bisphosphonates; Lepidium sativum; Osteoporosis; bone turnover markers