title: A comparative study of NONOate based NO donors: spermine NONOate is the best suited NO donor for angiogenesis.
abstract: Nitric oxide (NO) is a known modulator of angiogenesis. The NONOate subfamily of NO donors has long been used in experimental and clinical studies to promote angiogenesis. However, no studies have been conducted yet to compare the angiogenesis potential of these NO donors in respect to their pattern of NO release. We hypothesize that having different pattern of NO release, each of the NO donors in NONOate subfamily can promote key stages of angiogenesis in differential manner. To verify our hypothesis, NO donors with half life ranging from seconds to several hours and having very different pattern of NO release were selected to evaluate their efficacy in modulating angiogenesis. Endothelial tube formation using EAhy926 cells was maximally increased by Spermine NONOate (SP) treatment. SP treatment maximally induced both ex vivo and in vivo angiogenesis using egg yolk and cotton plug angiogenesis models respectively. Experiment using chick embryo partial ischemia model revealed SP as the best suited NO donor to recover ischemia driven hampered angiogenesis. The present study elaborated that differential release pattern of NO by different NO donors can modulate angiogenesis differentially and also suggested that SP have a unique pattern of NO release that best fits for angiogenesis.
authors: Vascular Biology Lab, AU-KBC Research Centre, Anna University, Chennai, India.
authors: Syamantak
authors: S
authors: Majumder
authors: Vascular Biology Lab, AU-KBC Research Centre, Anna University, Chennai, India.
authors: Swaraj
authors: S
authors: Sinha
authors: Vascular Biology Lab, AU-KBC Research Centre, Anna University, Chennai, India.
authors: Jamila H
authors: JH
authors: Siamwala
authors: Vascular Biology Lab, AU-KBC Research Centre, Anna University, Chennai, India.
authors: Ajit
authors: A
authors: Muley
authors: Vascular Biology Lab, AU-KBC Research Centre, Anna University, Chennai, India.
authors: Himabindu
authors: H
authors: Reddy Seerapu
authors: Vascular Biology Lab, AU-KBC Research Centre, Anna University, Chennai, India.
authors: Gopi Krishna
authors: GK
authors: Kolluru
authors: Vascular Biology Lab, AU-KBC Research Centre, Anna University, Chennai, India.
authors: Vimal
authors: V
authors: Veeriah
authors: Vascular Biology Lab, AU-KBC Research Centre, Anna University, Chennai, India.
authors: Shunmugam
authors: S
authors: Nagarajan
authors: Vascular Biology Lab, AU-KBC Research Centre, Anna University, Chennai, India.
authors: Sree Rama Chaitanya
authors: SR
authors: Sridhara
authors: Vascular Biology Lab, AU-KBC Research Centre, Anna University, Chennai, India.
authors: Mani Krishna
authors: MK
authors: Priya
authors: Vascular Biology Lab, AU-KBC Research Centre, Anna University, Chennai, India.
authors: Maniselvan
authors: M
authors: Kuppusamy
authors: Vascular Biology Lab, AU-KBC Research Centre, Anna University, Chennai, India.
authors: Sundaramoorthy
authors: S
authors: Srinivasan
authors: Vascular Biology Lab, AU-KBC Research Centre, Anna University, Chennai, India.
authors: Salini
authors: S
authors: Konikkat
authors: Vascular Biology Lab, AU-KBC Research Centre, Anna University, Chennai, India.
authors: Gowrishankar
authors: G
authors: Soundararajan
authors: Department of Pharmacology, C.L. Baid Metha College of Pharmacy, Chennai, India.
authors: S
authors: S
authors: Venkataraman
authors: Vascular Biology Lab, AU-KBC Research Centre, Anna University, Chennai, India.
authors: Uttara
authors: U
authors: Saran
authors: Vascular Biology Lab, AU-KBC Research Centre, Anna University, Chennai, India. Electronic address: soovro@yahoo.ca.
authors: Suvro
authors: S
authors: Chatterjee
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000737:chemistry*; D000818:Animals; D001011:Aorta / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D002642:Chick Embryo; D004530:Egg Yolk; D004730:Endothelium, Vascular / Q000378:metabolism; D020869:Gene Expression Profiling; D007511:Ischemia / Q000378:metabolism; D008297:Male; D018919:Neovascularization, Physiologic; D009569:Nitric Oxide / Q000737:chemistry; D020030:Nitric Oxide Donors / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D013096:Spermine / Q000031:analogs & derivatives* / Q000737:chemistry; D014945:Wound Healing
chemical_list: D043925:Angiogenesis Inducing Agents; D020030:Nitric Oxide Donors; C091861:spermine nitric oxide complex; D013096:Spermine; D009569:Nitric Oxide
keywords: Angiogenesis; Nitric oxide; Nitric oxide donors; cGMP