title: An inverse relationship between the expression of the gastric tumor suppressor RUNX3 and infection with Helicobacter pylori in gastric epithelial dysplasia.
abstract: OBJECTIVE
  This study was performed to determine the association between RUNX3 expression and Helicobacter pylori infection in premalignant gastric lesions.
  
  
  METHODS
  We examined 107 patients with gastric epithelial dysplasia who had undergone endoscopic mucosal resection or submucosal dissection. All tissue samples were evaluated by RUNX3 staining and subclassified by immunophenotype. H. pylori infection in dysplastic lesions and the normal surrounding tissue was examined by silver staining, and cagA status was assessed by polymerase chain reaction.
  
  
  RESULTS
  The loss of RUNX3 expression was observed in 62 cases (57.9%), and an association with H. pylori infection was found in 54 cases (50.5%). The infection rate with the cagA-positive H. pylori strain was 63.0%. In RUNX3-negative lesions, the rate of H. pylori infection (p=0.03) and the frequency of category 4 lesions (according to the revised Vienna classification) were high (p=0.02). In addition, the gastric mucin phenotype was predominant. In RUNX3-negative category 4 lesions, the rate of cagA-positive H. pylori infection rate was high but not significantly increased (p=0.08).
  
  
  CONCLUSIONS
  Infection with H. pylori is associated with inactivation of RUNX3 in early gastric carcinogenesis. This mechanism was prominent in gastric cancer with a gastric mucin phenotype.
authors: Department of Internal Medicine, St. Vincent's Hospital, The Catholic University of Korea College of Medicine, Suwon, Korea.
authors: Woo Chul
authors: WC
authors: Chung
authors: Sung Hoon
authors: SH
authors: Jung
authors: Kyu Re
authors: KR
authors: Joo
authors: Min Ji
authors: MJ
authors: Kim
authors: Gun Jung
authors: GJ
authors: Youn
authors: Yaeni
authors: Y
authors: Kim
authors: Joune Seup
authors: JS
authors: Lee
authors: Hyewon
authors: H
authors: Lee
authors: Ji Han
authors: JH
authors: Jung
authors: Yun Kyung
authors: YK
authors: Lee
mesh_terms: D000236:Adenoma / Q000737:chemistry*; D000368:Aged; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D002277:Carcinoma / Q000737:chemistry*; D002471:Cell Transformation, Neoplastic; D050677:Core Binding Factor Alpha 3 Subunit / Q000032:analysis*; D005260:Female; D005753:Gastric Mucosa / Q000737:chemistry* / Q000473:pathology; D016481:Helicobacter Infections / Q000378:metabolism*; D016480:Helicobacter pylori / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D055271:Mucin 5AC / Q000032:analysis; D055262:Mucin-2 / Q000032:analysis; D055272:Mucin-6 / Q000032:analysis; D015260:Neprilysin / Q000032:analysis; D010641:Phenotype; D011230:Precancerous Conditions / Q000737:chemistry* / Q000473:pathology; D013274:Stomach Neoplasms / Q000737:chemistry*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D050677:Core Binding Factor Alpha 3 Subunit; C524625:MUC2 protein, human; C524678:MUC5AC protein, human; C524734:MUC6 protein, human; D055271:Mucin 5AC; D055262:Mucin-2; D055272:Mucin-6; C493729:Runx3 protein, human; C080866:cagA protein, Helicobacter pylori; D015260:Neprilysin
keywords: CagA protein; Core binding factor alpha 3 subunit; Helicobacter pylori