title: Cross-reactivity between decellularized  porcine corneal lamellae for corneal  xenobridging and subsequent corneal  allotransplants.
abstract: The purpose of this study is to investigate cross-reactivity between hypertonic saline-treated decellularized porcine corneal lamellae for corneal xenobridging and subsequent corneal allotransplants. Five Chinese rhesus macaques, which had undergone anterior partial thickness corneal transplantation using hypertonic saline-treated decellularized porcine corneal lamellae in preceding experiments, were used as recipients for subsequent full-thickness corneal allografts. To determine whether sensitization of recipients to xenoantigens leads to cross-reactivity against alloantigens, we compared; (i) allogeneic one-way mixed lymphocyte reaction (MLR) of peripheral blood mononuclear cells (PBMCs) from xeno-sensitized recipients with that of PBMCs from naïve rhesus macaques, and (ii) amounts of IgG antibodies that bound to the PBMCs of a rhesus panel (five monkeys) before and after xeno-sensitization. Graft survival and immunologic profiles including memory T-cell subsets and donor rhesus-specific antibodies were also evaluated. No hyperacute or acute rejection was observed within a month of subsequent allotransplantation in any recipient. Alloreactivity by MLR was not different between xeno-sensitized rhesus recipients and naïve rhesus monkeys. Panel-reactive IgG antibodies were unchanged after xeno-sensitization, and no change in donor rhesus-specific antibodies was observed in any recipient. No significant changes in memory T-cell subsets were observed during the early post-operative period in any recipient. Decellularized porcine corneal lamellae may not increase cross-reactivity to alloantigens, and thus, porcine corneal lamellae may be used as a bridge to subsequent corneal allografting.
authors: Hyuk Jin
authors: HJ
authors: Choi
authors: Jong Joo
authors: JJ
authors: Lee
authors: Mee Kum
authors: MK
authors: Kim
authors: Hyun Ju
authors: HJ
authors: Lee
authors: Ah Young
authors: AY
authors: Ko
authors: Hee Jung
authors: HJ
authors: Kang
authors: Chung-Gyu
authors: CG
authors: Park
authors: Won Ryang
authors: WR
authors: Wee
mesh_terms: D064591:Allografts; D000818:Animals; D000906:Antibodies / Q000097:blood; D003315:Cornea / Q000276:immunology / Q000473:pathology*; D016039:Corneal Transplantation / Q000379:methods; D003429:Cross Reactions / Q000276:immunology*; D006084:Graft Rejection / Q000276:immunology*; D006085:Graft Survival / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000637:transplantation*; D008253:Macaca mulatta; D013552:Swine; D014183:Transplantation, Heterologous / Q000379:methods
chemical_list: D000906:Antibodies