title: Molecular and genetic analysis of the chloroplast ATPase of chlamydomonas.
abstract: We have carried out a molecular and genetic analysis of the chloroplast ATPase in Chlamydomonas reinhardtii. Recombination and complementation studies on 16 independently isolated chloroplast mutations affecting this complex demonstrated that they represent alleles in five distinct chloroplast genes. One of these five, the ac-u-c locus, has been positioned on the physical map of the chloroplast DNA by deletion mutations. The use of cloned spinach chloroplast ATPase genes in heterologous hybridizations to Chlamydomonas chloroplast DNA has allowed us to localize three or possibly four of the ATPase genes on the physical map. The beta and probably the epsilon subunit genes of Chlamydomonas CF1 lie within the same region of chloroplast DNA as the ac-u-c locus, while the alpha and proteolipid subunit genes appear to map adjacent to one another approximately 20 kbp away. Unlike the arrangement in higher plants, these two pairs of genes are separated from each other by an inverted repeat.
authors: Department of Zoology, Duke University, 27706, Durham, NC, U.S.A..
authors: J P
authors: JP
authors: Woessner
authors: A
authors: A
authors: Masson
authors: E H
authors: EH
authors: Harris
authors: P
authors: P
authors: Bennoun
authors: N W
authors: NW
authors: Gillham
authors: J E
authors: JE
authors: Boynton