title: Simultaneous determination of azoles antifungal drugs in chicken tissues by ultra-performance liquid chromatography tandem mass spectrometry.
abstract: An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established for the simultaneous determination of residues of enilconazole, parconazole, thiabendazole and its metabolite, 5-hydroxythiabendazole, in chicken tissues including liver, muscle and egg. Homogenized samples were extracted by acetonitrile, concentrated and purified by an Oasis MCX solid-phase extraction cartridge. Analysis of the target drugs was performed by liquid chromatography with an UPLCTM BEH C18 column, coupled with a tandem mass spectrometry (UPLC-MS/MS) operating in the positive multiple-reaction mode (MRM). Good linearity was obtained at concentrations of 0.10-100 μg kg(-1). The average recoveries of the analytes in chicken liver, muscle and egg at six fortification levels were in the range 93.6-119.5% with acceptable coefficients of variation (<14.8%). The detection capability (CCβ) of the analytes upon the method ranged from 0.05 to 138.1 μg kg(-1). This method was successfully applied in screening and confirming of target drugs in tens of real samples.
authors: Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control.
authors: Nan
authors: N
authors: Shang
authors: Jing
authors: J
authors: Zhang
authors: Bing
authors: B
authors: Shao
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000032:analysis*; D001393:Azoles / Q000032:analysis*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D008099:Liver / Q000737:chemistry*; D009132:Muscles / Q000737:chemistry*; D010063:Ovum / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D000935:Antifungal Agents; D001393:Azoles