title: pH-Dependent accumulation of indoleacetic acid by corn coleoptile sections.
abstract: The uptake of auxin by 1-mm slices of corn (Zea mays L.) coleoptiles, a tissue known to transport auxin polarly, depends on the pH of the medium. Short-term uptake of indole-3-acetic acid (IAA) in coleoptiles increases with decreasing pH of the buffer as would be expected if the undissociated weak acid, IAA·H, were more permeable than the auxin anion, IAA(-), and IAA(-) accumulates in the tissues because of the higher pH of the cytoplasm. Although uptake of [(3)H]IAA is reduced in neutral buffers, it is greater than expected if it were limited to just the extracellular space of the tissue. The radioactivity accumulated by the tissue can be quantitatively extracted by organic solvents and identified as IAA by thin-layer chromatography. The tissue radioactivity is freely mobile and can efflux from the tissue. Thus these cells in pH 5 buffer are able to retain an average internal concentration of mobile IAA that is at least several times greater than the external concentration. A prominent feature of auxin uptake from acidic buffers is enhanced accumulation at high auxin concentration. This indicates that, in addition to fluxes of IAA·H, a saturable site is involved in auxin uptake. Whenever the auxin-anion gradient is directed outward, saturating the efflux of auxin anions increases accumulation. Furthermore, the observed slowing of short-term uptake of radioactive IAA by increasing concentrations of IAA or K(+) indicates either an activation of the presumptive auxin leak or saturation of another carrier-mediated uptake system such as a symport of auxin anions with protons. By contrast in neutral buffers, effects of concentration on uptake rates disappear. This implies that at neutral pH the anion leak is decreased and influx depends on the symport.
authors: Department of Biology, Kline Biology Tower, Yale University, 06520, New Haven, CT, USA.
authors: K L
authors: KL
authors: Edwards
authors: M H
authors: MH
authors: Goldsmith