title: Effects of eugenol-reduced clove extract on glycogen phosphorylase b and the development of diabetes in db/db mice.
abstract: We found that the 50% aqueous EtOH extract of clove (Syzygium aromaticum) had potent dose-dependent inhibitory activity toward glycogen phosphorylase b and glucagon-stimulated glucose production in primary rat hepatocytes. Among the components, eugeniin inhibited glycogen phosphorylase b and glucagon-stimulated glucose production in primary rat hepatocytes, with IC50 values of 0.14 and 4.7 μM, respectively. In sharp contrast, eugenol showed no significant inhibition toward glycogen phosphorylase b, even at a concentration of 400 μM. Eugenol-reduced clove extracts (erCE) were prepared and when fed to a db/db mouse they clearly suppressed the blood glucose and HbA1c levels. Furthermore, plasma triglyceride and non-esterified fatty acid levels in 5% and 10% erCE-fed db/db mice were significantly lowered, compared with control db/db mice without erCE supplementation. These results suggested that dietary supplementation with the erCE could beneficially modify glucose and lipid metabolism and contribute to the prevention of the progress of hyperglycemia and metabolic syndrome.
authors: Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa 920-1181, Japan.
authors: Fujiko
authors: F
authors: Sanae
authors: Ogusa
authors: O
authors: Kamiyama
authors: Kyoko
authors: K
authors: Ikeda-Obatake
authors: Yasuhiko
authors: Y
authors: Higashi
authors: Naoki
authors: N
authors: Asano
authors: Isao
authors: I
authors: Adachi
authors: Atsushi
authors: A
authors: Kato
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy* / Q000201:enzymology* / Q000378:metabolism; D004195:Disease Models, Animal; D005054:Eugenol / Q000032:analysis* / Q000302:isolation & purification; D035264:Flowers / Q000737:chemistry; D006442:Glycated Hemoglobin / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism*; D024981:Glycogen Phosphorylase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D027842:Syzygium / Q000737:chemistry*
chemical_list: D001786:Blood Glucose; D005054:Eugenol; D006442:Glycated Hemoglobin; D006003:Glycogen; D024981:Glycogen Phosphorylase; D010936:Plant Extracts