title: Gig1, a novel antiviral effector involved in fish interferon response.
abstract: Vertebrate interferon (IFN) response defenses against viral infection through the induction of hundreds of IFN-stimulated genes (ISGs). Most ISGs are conserved across vertebrates; however, little is known about the species-specific ISGs. In this study, we reported that grass carp reovirus (GCRV)-induced gene 1 (Gig1), previously screened as a virus-induced gene from UV-inactivated GCRV-infected crucian carp (Carassius auratus) blastulae embryonic (CAB) cells, was a typical fish ISG, which was significantly induced by intracellular poly(I:C) through retinoic acid-inducible gene I (RIG-I)-like receptors-triggered IFN signaling pathway. Transient or stable overexpression of Gig1 prevented GCRV replication efficiently in cultured fish cells. Strikingly, Gig1 homologs were found exclusively in fish species forming a novel gene family. These results illustrate that there exists a Gig1 gene family unique to fish species and the founding gene mediates a novel fish IFN antiviral pathway.
authors: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan 430072, China.
authors: Fan
authors: F
authors: Sun
authors: Yi-Bing
authors: YB
authors: Zhang
authors: Jun
authors: J
authors: Jiang
authors: Bing
authors: B
authors: Wang
authors: Chen
authors: C
authors: Chen
authors: Jun
authors: J
authors: Zhang
authors: Jian-Fang
authors: JF
authors: Gui
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology* / Q000821:virology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006054:Goldfish / Q000235:genetics / Q000276:immunology* / Q000821:virology; D007372:Interferons / Q000276:immunology*; D008969:Molecular Sequence Data; D012087:Reoviridae / Q000276:immunology / Q000502:physiology*; D012088:Reoviridae Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D016415:Sequence Alignment; D015398:Signal Transduction
chemical_list: D029941:Fish Proteins; D007372:Interferons
keywords: Antiviral effects; Expression regulation; Fish; Gene family; Gig1; IFN response; RLR signaling pathway