title: Polysaccharide of Boschniakia rossica induces apoptosis on laryngeal carcinoma Hep2 cells.
abstract: The aim of this study was to explore the anti-tumor potential of a polysaccharide isolated from Boschniakia rossica (BRP) in Hep2 human larynx squamous carcinoma cells. High performance size-exclusion chromatography analysis showed that BRP was a homogeneous polysaccharide and had a molecular weight of 22 kDa. Total carbohydrate content in BRP was determined to be 96.9%, without the presence of protein and nucleic acid. BRP suppressed the proliferation of Hep2 cells in a time- and dose-dependent manner. Cell cycle analysis revealed that exposure to BRP (200 μg/ml) caused a G0/G1 cell cycle arrest in Hep2 cells. Moreover, treatment with BRP at 100-400 μg/ml for 24h induced a significant apoptosis Hep2 cells compared to untreated control cells, as determined by flow cytometry with annexin-V/propidium iodide double staining. Additionally, BRP treatment promoted the cleavage of pro-caspase-3, pro-caspase-8, and pro-caspase-9, coupled with increased expression of death receptor DR5 and Bax and reduced expression of Bcl-2. Taken together, our data demonstrate that BRP shows potent anti-tumor activity in human larynx squamous carcinoma, largely through induction of G0/G1 cell cycle arrest and apoptosis. Activation of both mitochondria-mediated and death receptor-mediated apoptosis pathways is involved in the cytotoxicity of BRP.
authors: Department of Otolaryngology-Head and Neck Surgery, The Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors: Zhenghui
authors: Z
authors: Wang
authors: Cancer Center of Henan Province People's Hospital, Zhengzhou 450000, China.
authors: Chuangxin
authors: C
authors: Lu
authors: Department of Otolaryngology-Head and Neck Surgery, The Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China. Electronic address: caiqinwu11@163.com.
authors: Caiqin
authors: C
authors: Wu
authors: Department of Otolaryngology-Head and Neck Surgery, The Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors: Min
authors: M
authors: Xu
authors: Department of Otolaryngology-Head and Neck Surgery, The Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors: Xiaohui
authors: X
authors: Kou
authors: Department of Otolaryngology-Head and Neck Surgery, The Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors: Demin
authors: D
authors: Kong
authors: Department of Surgery and Anesthesia, The Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors: Gangli
authors: G
authors: Jing
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D007822:Laryngeal Neoplasms / Q000188:drug therapy / Q000473:pathology*; D031670:Orobanchaceae / Q000737:chemistry*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D010936:Plant Extracts; D011134:Polysaccharides
keywords: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; Apoptosis; B. rossica; BRP; BSA; Boschniakia rossica; Cell cycle arrest; Chemotherapy; ConA; DEAE; DMSO; FCS; FITC; HPGPC; Hep2; MTT; OVA; Polysaccharide; SCC; TBS; TCMs; Tris buffered solution; boschniakia rossica; bovine serum albumin; concanavalin A, LPS, lipopolysaccharide; diethylaminoethyl; dimethyl sulfoxide; fetal calf serum; fluorescein isothiocyanate; high performance size-exclusion chromatography; ovalbumin; squamous cell carcinoma; traditional Chinese medicines