title: Analysis of microRNA and mRNA expression profiles highlights alterations in modulation of the MAPK pathway under octanal exposure.
abstract: Previous environmental microRNA (miRNA) studies have investigated a limited number of candidate miRNAs and have not evaluated functional effects on gene expression. In this study, we aimed to identify octanal (OC)-sensitive miRNAs and to characterize the relationships between miRNAs and expression of candidate genes involved in OC-induced toxicity. Microarray analysis identified 15 miRNAs that were differentially expressed in OC-exposed A549 human alveolar cells. Integrated analyses of miRNA and mRNA expression profiles identified significant miRNA-mRNA anti-correlations. GO analysis of 101 putative target genes showed that the biological category 'MAPK signaling pathway' was prominently annotated. Moreover, we detected increased phosphorylation of p38 MAPK in the OC-exposed group. By integrating the transcriptome and microRNAome, we provide evidence that OC can affect MAPK-induced toxicity signaling. Therefore, this study demonstrates the added value of an integrated miRNA-mRNA approach for identifying molecular events induced by environmental pollutants in an in vitro human model.
authors: Center for Integrated Risk Research, Cellular and Molecular Toxicology Laboratory, Korea Institute of Science & Technology P.O. Box 131, Cheongryang, Seoul 130-650, Korea; School of Life Sciences and Biotechnology, Korea University, Anam-Dong, Seoungbuk-Gu, Seoul 136-791, Korea.
authors: Mi-Kyung
authors: MK
authors: Song
authors: Center for Integrated Risk Research, Cellular and Molecular Toxicology Laboratory, Korea Institute of Science & Technology P.O. Box 131, Cheongryang, Seoul 130-650, Korea.
authors: Han-Seam
authors: HS
authors: Choi
authors: Center for Integrated Risk Research, Cellular and Molecular Toxicology Laboratory, Korea Institute of Science & Technology P.O. Box 131, Cheongryang, Seoul 130-650, Korea.
authors: Hyo-Sun
authors: HS
authors: Lee
authors: Department of Marine Sciences, Incheon National University, 12-1 Songdo-dong, Yeonsu-gu, Incheon 406-772, Korea.
authors: Youn-Jung
authors: YJ
authors: Kim
authors: School of Life Sciences and Biotechnology, Korea University, Anam-Dong, Seoungbuk-Gu, Seoul 136-791, Korea.
authors: Yong-Keun
authors: YK
authors: Park
authors: Center for Integrated Risk Research, Cellular and Molecular Toxicology Laboratory, Korea Institute of Science & Technology P.O. Box 131, Cheongryang, Seoul 130-650, Korea. Electronic address: ryujc@kist.re.kr.
authors: Jae-Chun
authors: JC
authors: Ryu
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D000447:Aldehydes / Q000633:toxicity*; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism*
chemical_list: D000393:Air Pollutants; D000447:Aldehydes; D035683:MicroRNAs; D012333:RNA, Messenger; D020928:Mitogen-Activated Protein Kinases; C031639:caprylic aldehyde
keywords: Aldehydes; MicroRNA; Microarray; Mitogen-activated protein kinase; Octanal; Target mRNAs