title: Synergistic effect of 5-HT2A receptor gene and MAOA gene on the negative emotion of patients with depression.
abstract: OBJECTIVE
  To analyse the synergistic effect of polymorphism of the tandem repeat sequence u-VNTR of 5-hydroxytryptamine 2A (5-HT2A) receptor gene and monoamine oxidase A (MAOA) gene on the negative emotion in frontal lobe of patients with depression through functional magnetic resonance imaging (fMRI) technique.
  
  
  METHODS
  Functional magnetic resonance imaging scanning was performed for 72 patients with depression and 70 gender, age-matched healthy people with physical examination under negative emotion recognition task. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was adopted to analyse genotype. The superior, middle and inferior gyrus of bilateral frontal lobe was regarded as the brain region of interest, and then the difference of activation intensity in frontal lobe subregion between control groups and patient groups with different genotypes, and the interaction between the two kinds of polymorphism were compared.
  
  
  RESULTS
  The activation intensity in right frontal middle gyrus of patients with CC genotype increased obviously compared with TT and TC genotype patient groups and TT genotype control group (P<0·01); the activation intensity in right frontal inferior gyrus of patients with CC genotype increased obviously compared with TT and TC genotype patient groups and control groups (P<0·01); the activation intensity in right frontal middle gyrus and left frontal inferior gyrus of patients with MAOA high-activity genotype increased obviously compared with patient and control groups with MAOA low-activity genotype (P<0·01). In sum, there existed synergistic effect of the two genotypes on the activation abnormality of negative emotion recognition in right frontal middle gyrus (F = 6·18, P = 0·029). The negative activation in right frontal middle gyrus of patients with CC+H genotypes increased most obviously (P<0·05).
  
  
  CONCLUSIONS
  The frontal abnormality of patients with depression had certain 5-HT genetic basis, and 5-HT2A receptor CC allele and MAOA-H genotype had synergistic effect on the activity abnormality when recognizing negative emotion in right frontal middle gyrus of patients with depression.
authors: The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors: Huirong
authors: H
authors: Guo
authors: Yuming
authors: Y
authors: Ren
authors: Ning
authors: N
authors: Zhao
authors: Yali
authors: Y
authors: Wang
authors: Shuying
authors: S
authors: Li
authors: He
authors: H
authors: Cui
authors: Sijia
authors: S
authors: Zhang
authors: Jianhua
authors: J
authors: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D003863:Depression / Q000235:genetics*; D004644:Emotions; D005260:Female; D005625:Frontal Lobe / Q000503:physiopathology*; D005838:Genotype; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008875:Middle Aged; D008995:Monoamine Oxidase / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D044402:Receptor, Serotonin, 5-HT2A / Q000235:genetics*
chemical_list: D044402:Receptor, Serotonin, 5-HT2A; D008995:Monoamine Oxidase
keywords: 5-hydroxytryptamine 2A receptor gene; depression; frontal; functional magnetic resonance imaging; monoamine oxidase A