title: Evaluation of canine bone marrow-derived mesenchymal stem cells after long-term cryopreservation.
abstract: Autologous bone marrow-derived mesenchymal stem cell (BMSCs)-based therapies show great potential in regenerative medicine. However, long-term storage and preservation of BMSCs for clinical use is still a great clinical challenge. The present study aimed to analyze the effect of long-term cryopreservation on the regenerative ability of BMSCs. After cryopreservation of BMSCs from beagle dogs for three years, cell viability, and quantitative analysis of alkaline phosphatase (ALP) activity, surface adherence, and mineralized nodule formation were analyzed. BMSCs in cell-scaffold complex were then implanted into nude mice. There was no significant difference in cell viability and ALP activity between osteogenic differentiation and non-osteogenic differentiation of BMSCs, and BMSCs in cell-scaffold complex retained osteogenic differentiation ability in vivo. These results indicate that long-term cryopreserved BMSCs maintain their have capacity to contribute to regeneration.
authors: 1 School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, China.
authors: Xiaofeng
authors: X
authors: Zhu
authors: Fang
authors: F
authors: Yuan
authors: Houxuan
authors: H
authors: Li
authors: Yuqian
authors: Y
authors: Zheng
authors: Yin
authors: Y
authors: Xiao
authors: Fuhua
authors: F
authors: Yan
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002470:Cell Survival; D003094:Collagen / Q000378:metabolism; D015925:Cryopreservation / Q000662:veterinary*; D004285:Dogs; D015971:Gene Expression Regulation, Enzymologic; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D051379:Mice; D008819:Mice, Nude; D010012:Osteogenesis / Q000502:physiology; D012662:Semen Preservation / Q000662:veterinary*; D013997:Time Factors
chemical_list: D003094:Collagen; D000469:Alkaline Phosphatase