title: Development of immobilized cellulase through functionalized gold nano-particles for glucose production by continuous hydrolysis of waste bamboo chopsticks.
abstract: Cellulase immobilized on silica through the assistance of l-cysteine functionalized gold nano-particle was applied for the continuous hydrolysis of waste bamboo chopsticks powder to produce glucose. The optimal conditions for the continuous hydrolysis were pH 8.0, 50°C. A 4-day reaction with an initial 0.3 gL⁻¹ waste bamboo chopsticks powder, a feed containing 0.2 gL⁻¹ waste bamboo chopsticks powder at a continuous feed and draw rate of 0.5 mLmin⁻¹, and an enzyme loading of 40 mgcellulase(gsilica)⁻¹, has 72.0-76.6% conversion rates of repeated hydrolyses that correspond to a total production of 630.5-671.2mg glucose and are much better than batch hydrolyses. At higher enzyme loading (117 mgcellulase(gsilica)⁻¹), higher initial concentration (0.5 gL⁻¹), and higher feed concentration (0.42 gL⁻¹) the conversion rate increases to 82.9% and a total amount of 1418 mgglucose. The immobilized cellulase can be recovered easily by filtration and used repeatedly at least 6 times over a period more than 90 days with a recovered activity approximately the same as or better than previous reactions. Thus the process is promising for scaling up.
authors: Department of Chemistry and Research Center for Analysis and Identification, Chung Yuan Christian University, 200 Chung Pei Road, Chungli 32023, Taiwan, ROC. Electronic address: chengce@cycu.edu.tw.
authors: Cheanyeh
authors: C
authors: Cheng
authors: Kuo-Chung
authors: KC
authors: Chang
mesh_terms: D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation; D002480:Cellulase; D004800:Enzymes, Immobilized; D005947:Glucose / Q000096:biosynthesis*; D006046:Gold; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D053768:Metal Nanoparticles; D059205:Renewable Energy; D031787:Sasa; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D006046:Gold; D002480:Cellulase; D005947:Glucose
keywords: Continuous hydrolysis; Functionalized gold nano-particle; Glucose; Immobilized cellulose; Silica; Waste bamboo chopsticks powder