title: Screening assays to identify artificial glmS ribozyme activators.
abstract: Ribsowitches are putative drug targets as they often regulate the expression of essential bacterial genes. This finding necessitates the development of suitable assays, at best high-throughput (HT) compatible, which allow the screening of compound libraries for riboswitch activation. Here, we describe a HT-compatible fluorescence-based screening assay employing a minimal core motif of the Bacillus subtilis glmS riboswitch and the metabolite-induced self-cleavage assay using the full-length glmS ribozyme of Staphylococcus aureus for the identification of artificial molecules activating this regulatory RNA.
authors: Life and Medical Sciences Institute, University of Bonn, Bonn, Germany.
authors: Christina E
authors: CE
authors: Lünse
authors: Günter
authors: G
authors: Mayer
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D057166:High-Throughput Screening Assays; D008967:Molecular Biology / Q000379:methods; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000235:genetics*; D058928:Riboswitch / Q000235:genetics; D013211:Staphylococcus aureus / Q000235:genetics; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)
chemical_list: D001426:Bacterial Proteins; D016337:RNA, Catalytic; D058928:Riboswitch; C000731191:component S, glutamate mutase protein, Bacteria; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)