title: Analysis of superoxide dismutase 1, dual-specificity phosphatase 1, and transforming growth factor, beta 1 genes expression in keratoconic and non-keratoconic corneas.
abstract: OBJECTIVE
  To quantitatively assess the superoxide dismutase 1 (SOD1), transforming growth factor, beta 1 (TGF-β1), and dual-specificity phosphatase 1 (DUSP1) messenger ribonucleic acid (mRNA) expression levels as the main intracellular reactive oxygen species neutralizers, wound healing mediators, and immunomodulators (respectively) in keratoconic (KCN) and non-KCN corneas.
  
  
  METHODS
  Total RNA was extracted from normal and keratoconic cultured corneal stromal fibroblasts. Semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used to measure the relative expression levels of mRNAs of the SOD1, TGF-β1, and DUSP1 genes.
  
  
  RESULTS
  The mRNA expression of TGF-β1 and DUSP1 was augmented in the KCN corneas (three- and fivefold, respectively; both p<0.05). The KCN and non-KCN samples showed no difference in comparative SOD1 mRNA levels.
  
  
  CONCLUSIONS
  This study demonstrated a higher level of DUSP1 and TGF-β1 expression as known molecules in the inflammatory process. These results may provide new insight into the complex molecular pathways underlying KCN for investigating other inflammatory molecules.
authors: Noor Ophthalmology Research Center, Noor Eye hospital, Tehran, Iran.
authors: Samira
authors: S
authors: Saee-Rad
authors: Reza
authors: R
authors: Raoofian
authors: Mirgholamreza
authors: M
authors: Mahbod
authors: Mohammad
authors: M
authors: Miraftab
authors: Majid
authors: M
authors: Mojarrad
authors: Soheila
authors: S
authors: Asgari
authors: Farhad
authors: F
authors: Rezvan
authors: Hassan
authors: H
authors: Hashemi
mesh_terms: D002478:Cells, Cultured; D003315:Cornea / Q000276:immunology / Q000473:pathology / Q000601:surgery; D054638:Dual Specificity Phosphatase 1 / Q000235:genetics* / Q000276:immunology; D005260:Female; D005347:Fibroblasts / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000601:surgery; D007640:Keratoconus / Q000235:genetics* / Q000276:immunology / Q000473:pathology / Q000601:surgery; D015948:Keratoplasty, Penetrating; D008297:Male; D012333:RNA, Messenger / Q000235:genetics* / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000235:genetics / Q000276:immunology; D000072105:Superoxide Dismutase-1; D053773:Transforming Growth Factor beta1 / Q000235:genetics* / Q000276:immunology
chemical_list: D012333:RNA, Messenger; C000606290:SOD1 protein, human; D053773:Transforming Growth Factor beta1; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; C517019:DUSP1 protein, human; D054638:Dual Specificity Phosphatase 1