title: Heading for an economic industrial upgrading of crude glycerol from biodiesel production to 1,3-propanediol by Lactobacillus diolivorans.
abstract: Lactobacillus diolivorans was evaluated as a potential organism for production of 1,3-propanediol under industrially relevant conditions. Crude glycerol of different origins has been tested and showed no inhibitory effects on growth or production. Using crude glycerol from biodiesel production from palm oil 85 g/l 1,3-propanediol have been obtained with a productivity of 0.45 g/lh in a fed-batch cultivation. Sugar necessary for the formation of biomass was replaced with a hydrolysate from lignocellulosic material resulting in 75 g/l 1,3-propanediol and a productivity of 0.36 g/lh. Lignocellulosic hydrolysate contained the potential inhibitors furfural and 5-hydroxymethylfurfural at concentrations of 0.7 and 0.3 g/l, respectively. Addition of furfural and 5-hydroxymethylfurfural to batch cultures in said concentrations did not show inhibitory effects on growth or 1,3-propanediol production.
authors: School of Bioengineering, FH Campus Wien - University of Applied Sciences, Muthgasse 62, 1190 Vienna, Austria; Department of Biotechnology, BOKU - VIBT University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria.
authors: Stefan
authors: S
authors: Pflügl
authors: Department of Biotechnology, BOKU - VIBT University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria. Electronic address: hans.marx@boku.ac.at.
authors: Hans
authors: H
authors: Marx
authors: Department of Biotechnology, BOKU - VIBT University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria; Austrian Centre of Industrial Biotechnology (ACIB GmbH), Muthgasse 11, 1190 Vienna, Austria.
authors: Diethard
authors: D
authors: Mattanovich
authors: Department of Biotechnology, BOKU - VIBT University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria; Austrian Centre of Industrial Biotechnology (ACIB GmbH), Muthgasse 11, 1190 Vienna, Austria.
authors: Michael
authors: M
authors: Sauer
mesh_terms: D061249:Batch Cell Culture Techniques; D056804:Biofuels / Q000191:economics* / Q000382:microbiology; D018533:Biomass; D002241:Carbohydrates / Q000494:pharmacology; D005990:Glycerol / Q000191:economics* / Q000378:metabolism* / Q000494:pharmacology; D006868:Hydrolysis / Q000187:drug effects; D007218:Industrial Microbiology / Q000191:economics*; D007778:Lactobacillus / Q000187:drug effects / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism; D011409:Propylene Glycols / Q000191:economics* / Q000378:metabolism*; D013997:Time Factors; D014934:Wood / Q000187:drug effects
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D011409:Propylene Glycols; C036909:lignocellulose; C041787:1,3-propanediol; D008031:Lignin; D005990:Glycerol
keywords: 1,3-Propanediol; Industrial production; Inhibitory effects by crude substrates; Lignocellulosic hydrolysate; Microbial conversion of crude glycerol