title: Production and characterization of a biosurfactant from Cyberlindnera samutprakarnensis JP52(T.).
abstract: Cyberlindnera samutprakarnensis JP52(T), isolated from cosmetic industrial wastes in Thailand, was found to be an efficient biosurfactant-producing yeast when cultured in a medium containing (2% (w/v) glucose and 2% (v/v) palm oil at 30 °C, 200 rpm for 7 d. The crude biosurfactant had the ability to reduce the surface tension from 55.7 to 30.9 mN/m at 25 °C with a critical micelle concentration (CMC) of 0.046%. Physicochemical analysis of the crude biosurfactant revealed that it had wide ranges of optimum pH and pH stability at 6-9 and 3-10 respectively. It was also thermostable and retained 80% activity even after heat treatment, and it tolerated NaCl at 1.0-10%. Furthermore, it effectively emulsified various vegetable oils with an E24 value of over 80%. A partially purified biosurfactant fraction was analyzed for its structure by MALDI-TOF MS and NMR. This revealed that the biosurfactant mainly contained sophorolipids in C18-(MW 574) and C16-diaceltylated (MW 662) forms.
authors: Department of Microbiology, Faculty of Science, Chulalongkorn University.
authors: Jamroonsri
authors: J
authors: Poomtien
authors: Jiraporn
authors: J
authors: Thaniyavarn
authors: Pairoh
authors: P
authors: Pinphanichakarn
authors: Sasitorn
authors: S
authors: Jindamorakot
authors: Masaaki
authors: M
authors: Morikawa
mesh_terms: D002244:Carbon / Q000378:metabolism; D006017:Glycolipids / Q000096:biosynthesis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008823:Micelles; D004718:Saccharomycetales / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013501:Surface-Active Agents / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature
chemical_list: D006017:Glycolipids; D008823:Micelles; D013501:Surface-Active Agents; D012965:Sodium Chloride; D002244:Carbon