title: Proteomics analysis of alfalfa response to heat stress.
abstract: The proteome responses to heat stress have not been well understood. In this study, alfalfa (Medicago sativa L. cv. Huaiyin) seedlings were exposed to 25 °C (control) and 40 °C (heat stress) in growth chambers, and leaves were collected at 24, 48 and 72 h after treatment, respectively. The morphological, physiological and proteomic processes were negatively affected under heat stress. Proteins were extracted and separated by two-dimensional polyacrylamide gel electrophoresis (2-DE), and differentially expressed protein spots were identified by mass spectrometry (MS). Totally, 81 differentially expressed proteins were identified successfully by MALDI-TOF/TOF. These proteins were categorized into nine classes: including metabolism, energy, protein synthesis, protein destination/storage, transporters, intracellular traffic, cell structure, signal transduction and disease/defence. Five proteins were further analyzed for mRNA levels. The results of the proteomics analyses provide a better understanding of the molecular basis of heat-stress responses in alfalfa.
authors: College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
authors: Weimin
authors: W
authors: Li
authors: Zhenwu
authors: Z
authors: Wei
authors: Zhihong
authors: Z
authors: Qiao
authors: Zinian
authors: Z
authors: Wu
authors: Lixiang
authors: L
authors: Cheng
authors: Yuyang
authors: Y
authors: Wang
mesh_terms: D000220:Adaptation, Biological; D018506:Gene Expression Regulation, Plant; D006358:Hot Temperature; D000455:Medicago sativa / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010641:Phenotype; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D020543:Proteome; D040901:Proteomics; D012333:RNA, Messenger / Q000235:genetics; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D020543:Proteome; D012333:RNA, Messenger