title: Comparison of immunomodulating properties of Beta-lactoglobulin and its hydrolysates.
abstract: Cow's milk allergy is one of the most common food allergies in childhood. Beta-lactoglobulin (β- lg) is a dominant allergen in cow's milk. Hydrolysis is known as an effective method to reduce the allergenicity of proteins. Thus, the objective of this study was to compare the allergenicity of β-lg and its hydrolysates using an animal model. Twenty four BALB/c mice were divided into three groups and subcutaneously injected with native bovine β-lg and its hydrolysates on days 0, 7 and 14. During the sensitization period, a number of systemic anaphylactic indicators were observed in mice sensitized by β-lg compared to those sensitized by hydrolysates of β-lg. Mice sensitized by hydrolysates of β-lg showed a significantly lower spleen lymphocyte proliferation level than that sensitized by intact β-lg. Antibody levels of β-lg-specific IgE in serum induced by native β-lg were significantly high. Plasma histamine levels were also evaluated and showed the same trend as IgE. Moreover, the hydrolysates of β-lg significantly down-regulated IL-4 and IL-5 secretions in serum. These results suggested that enzymatic hydrolysis could reduce the allergenicity of β-lg.
authors: Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China. duancui19841111@163.com.
authors: Cui-cui
authors: CC
authors: Duan
authors: Ai-li
authors: AL
authors: Li
authors: Li-jie
authors: LJ
authors: Yang
authors: Rui
authors: R
authors: Zhao
authors: Wen-guang
authors: WG
authors: Fan
authors: Gui-cheng
authors: GC
authors: Huo
mesh_terms: D000818:Animals; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects / Q000276:immunology; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007155:Immunologic Factors / Q000276:immunology / Q000494:pharmacology*; D015847:Interleukin-4 / Q000276:immunology; D015848:Interleukin-5 / Q000276:immunology; D007782:Lactoglobulins / Q000276:immunology / Q000494:pharmacology*; D008214:Lymphocytes / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D016269:Milk Hypersensitivity / Q000276:immunology* / Q000473:pathology; D011492:Protein Hydrolysates / Q000276:immunology / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology* / Q000473:pathology
chemical_list: D007155:Immunologic Factors; D015848:Interleukin-5; D007782:Lactoglobulins; D011492:Protein Hydrolysates; D015847:Interleukin-4; D007073:Immunoglobulin E