title: De novo biosynthesis of linoleic acid and its conversion to the hydrocarbon (Z,Z)-6,9-heptadecadiene in the astigmatid mite, Carpoglyphus lactis: incorporation experiments with 13C-labeled glucose.
abstract: De novo biosynthesis of linoleic acid (LA) and its conversion to (Z,Z)-6,9-heptadecadiene were examined in Carpoglyphus lactis (Acarina, Carpoglyphidae). Experiments involving (13)C-administration using [1-(13)C]-d-glucose revealed that (13)C atoms were incorporated into LA of total lipid extracted from the mite, resulting in labeling of all even-numbered carbons. This result demonstrated that LA was produced from (13)C-labeled acetyl-CoA, which is indicative of direct de novo biosynthesis. In these feeding experiments involving [1-(13)C]-D-glucose, (13)C atoms were also incorporated into (Z,Z)-6,9-heptadecadiene, which is one of the major secretory components in the mite. The labeling pattern of (Z,Z)-6,9-heptadecadiene at odd-numbered carbons agreed well with that of LA after loss of the carboxyl carbon. It was concluded that the mites could stably convert LA into (Z,Z)-6,9-heptadecadiene without the dietary requirement of this essential fatty acid.
authors: Faculty of Bioenvironmental Science, Kyoto Gakuen University, 1-1 Nanjo, Sogabe, Kameoka 621-8555, Japan. Electronic address: shimizu@kyotogakuen.ac.jp.
authors: Nobuhiro
authors: N
authors: Shimizu
authors: Faculty of Bioenvironmental Science, Kyoto Gakuen University, 1-1 Nanjo, Sogabe, Kameoka 621-8555, Japan.
authors: Michiya
authors: M
authors: Naito
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
authors: Naoki
authors: N
authors: Mori
authors: Asano Active Enzyme Molecule Project, JST, ERATO, Kyoto Brunch, Kyoto 602-0841, Japan; Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan.
authors: Yasumasa
authors: Y
authors: Kuwahara
mesh_terms: D000466:Alkadienes / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D053898:Biosynthetic Pathways; D008401:Gas Chromatography-Mass Spectrometry; D019787:Linoleic Acid / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D008925:Mites / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D000466:Alkadienes; D019787:Linoleic Acid
keywords: (Z,Z)-6,9-heptadecadiene; Astigmata; Carpoglyphus lactis; De novo biosynthesis; Essential fatty acid; Linoleic acid