title: Nanodiamonds with silicon vacancy defects for nontoxic photostable fluorescent labeling of neural precursor cells.
abstract: Nanodiamonds (NDs) containing silicon vacancy (SiV) defects were evaluated as a potential biomarker for the labeling and fluorescent imaging of neural precursor cells (NPCs). SiV-containing NDs were synthesized using chemical vapor deposition and silicon ion implantation. Spectrally, SiV-containing NDs exhibited extremely stable fluorescence and narrow bandwidth emission with an excellent signal to noise ratio exceeding that of NDs containing nitrogen-vacancy centers. NPCs labeled with NDs exhibited normal cell viability and proliferative properties consistent with biocompatibility. We conclude that SiV-containing NDs are a promising biomedical research tool for cellular labeling and optical imaging in stem cell research.
authors: Tobias D
authors: TD
authors: Merson
authors: Stefania
authors: S
authors: Castelletto
authors: Igor
authors: I
authors: Aharonovich
authors: Alisa
authors: A
authors: Turbic
authors: Trevor J
authors: TJ
authors: Kilpatrick
authors: Ann M
authors: AM
authors: Turnley
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D058612:Nanodiamonds / Q000737:chemistry*; D058953:Neural Stem Cells / Q000378:metabolism*; D012825:Silicon / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D058612:Nanodiamonds; D012825:Silicon