title: A one-pot system for production of L-2-aminobutyric acid from L-threonine by L-threonine deaminase and a NADH-regeneration system based on L-leucine dehydrogenase and formate dehydrogenase.
abstract: L-2-Aminobutyric acid (L-ABA) is an unnatural amino acid that is a key intermediate for the synthesis of several important drugs. It can be produced by transaminase or dehydrogenase from α-ketobutyric acid, which can be synthesized enzymatically from the bulk amino acid, L-threonine. Deamination of L-threonine followed by a hydrogenation reaction gave almost the theoretical yield and was estimated to be more cost-effective than the established chemical process. L-Threonine deaminase from Escherichia coli, L-leucine dehydrogenase from Bacillus cereus, and formate dehydrogenase from Pseudomonas sp. were over-expressed in E. coli and used for one-pot production of L-ABA with formate as a co-substrate for NADH regeneration. 30 mol L-threonine were converted to 29.2 mol L-ABA at 97.3 % of theoretical yield and with productivity of 6.37 g l(-1) h(-1) at 50 l. This process offers a promising approach to fulfil industrial requirements for L-ABA.
authors: Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China, rstao@cibt.ac.cn.
authors: Rongsheng
authors: R
authors: Tao
authors: Yu
authors: Y
authors: Jiang
authors: Fuyun
authors: F
authors: Zhu
authors: Sheng
authors: S
authors: Yang
mesh_terms: D000613:Aminobutyrates / Q000378:metabolism*; D001409:Bacillus cereus / Q000201:enzymology / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005560:Formate Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D050803:Leucine Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013912:Threonine / Q000378:metabolism*; D013913:Threonine Dehydratase / Q000235:genetics / Q000378:metabolism*
chemical_list: D000613:Aminobutyrates; D011994:Recombinant Proteins; D009243:NAD; D013912:Threonine; C012223:alpha-aminobutyric acid; D005560:Formate Dehydrogenases; D050803:Leucine Dehydrogenase; D013913:Threonine Dehydratase