title: Characteristics of cardiac cell cultures derived from human myocardial explants.
abstract: Primary cell cultures derived from human myocardial explants were obtained and characterized. The explant cultures contained cardiac stem cells (c-kit(+); ≈ 4%), microvascular cells (endothelial cells and pericytes), fibroblasts, and myofibroblasts. It was demonstrated that culturing of cardiac cells in cardiospheres did not promote enrichment of the cell culture with stem cells. MACS-sorted c-kit(+) cells from the explant culture were characterized by limited proliferative capacity and were capable of cardiomyogenic differentiation. The presence of microvascular cells determined general angiogenic potential of the culture.
authors: Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk; E. N. Meshalkin Research Institute of Circulatory Pathology, Ministry of Health of the Russian Federation, Novosibirsk; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia. zakian@bionet.nsc.ru.
authors: S V
authors: SV
authors: Pavlova
authors: P P
authors: PP
authors: Perovskii
authors: E V
authors: EV
authors: Chepeleva
authors: A A
authors: AA
authors: Malakhova
authors: E V
authors: EV
authors: Dement'eva
authors: E A
authors: EA
authors: Pokushalov
authors: G T
authors: GT
authors: Sukhikh
authors: S M
authors: SM
authors: Zakiyan
mesh_terms: D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006801:Humans; D018189:Immunomagnetic Separation; D007335:Insulin-Like Growth Factor II / Q000378:metabolism; D009206:Myocardium / Q000473:pathology; D032383:Myocytes, Cardiac / Q000502:physiology*; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism; D017526:Receptor, IGF Type 1 / Q000378:metabolism; D017527:Receptor, IGF Type 2 / Q000378:metabolism; D012038:Regeneration; D018874:Spheroids, Cellular / Q000502:physiology; D013234:Stem Cells / Q000502:physiology; D046509:Tissue Culture Techniques
chemical_list: C066855:HGF protein, human; C035170:IGF2 protein, human; D017527:Receptor, IGF Type 2; D017228:Hepatocyte Growth Factor; D007335:Insulin-Like Growth Factor II; D019859:Proto-Oncogene Proteins c-met; D017526:Receptor, IGF Type 1