title: Dual substrate strategy to enhance butanol production using high cell inoculum and its efficient recovery by pervaporation.
abstract: The present study deals with the development of an efficient ABE fermentation process using mixed substrate strategy for butanol production wherein no acetone was produced. For this, glucose was supplemented in the medium containing glycerol as main substrate which leads to a higher butanol production of 17.75 g/L in 72 h by Clostridium acetobutylicum KF158795. Moreover, the high cell inoculum also resulted in an increased ABE productivity of 0.46 g/L/h. Further, industrial scalability of the process was also successfully validated in a 300 L fermenter. Furthermore, potential of the Polymeric (PolyRMem) and Zeolite (ZeoMem) membranes for separation of butanol from fermentation broth was also studied by testing the pervaporation performance through which the butanol was successfully recovered.
authors: Department of Microbiology, University of Delhi South Campus, New Delhi 110021, India.
authors: Sweta
authors: S
authors: Yadav
authors: Department of Microbiology, University of Delhi South Campus, New Delhi 110021, India.
authors: Garima
authors: G
authors: Rawat
authors: Department of Microbiology, University of Delhi South Campus, New Delhi 110021, India.
authors: Priyanka
authors: P
authors: Tripathi
authors: Department of Microbiology, University of Delhi South Campus, New Delhi 110021, India. Electronic address: rks.micro2012@gmail.com.
authors: R K
authors: RK
authors: Saxena
mesh_terms: D000096:Acetone / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology; D000440:Butanols / Q000378:metabolism*; D046969:Clostridium acetobutylicum / Q000166:cytology* / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D017550:Spectroscopy, Fourier Transform Infrared; D014835:Volatilization
chemical_list: D000440:Butanols; D008567:Membranes, Artificial; D000096:Acetone; D000431:Ethanol; D005947:Glucose
keywords: 300L fermenter; Clostridium acetobutylicum KF158795; Glucose; Glycerol; Pervaporation