title: Direct proteomic mapping of Streptomyces roseosporus NRRL 11379 with precursor and insights into daptomycin biosynthesis.
abstract: This first-attempt study provided liquid chromatography tandem mass (LC-MS/MS) proteomics approach to explore precursor effects on daptomycin synthesis from Streptomyces roseosporus NRRL 11379. Among all, 357 and 691 differential proteins from 601 proteins in precursor group (144 h+) and 935 proteins in non-precursor group (144 h-) were identified, respectively. Through the simulation of the 2D-protein mapping, most proteins were found in isoelectric points ranged of 4.5-10.0 as well as Mws ranged 10-100 kDa. As a result, LC-MS/MS analysis was consistence with the analytical results of two-dimensional electrophoresis (2DE) but provided much intact profiles of proteins by precursor effect on S. roseosporus. To have more insight exploration, differential proteins associated to Streptomyces spp. were defined into 14 groups of their functional classification. The major differential proteins were in transport/membrane functional group with an occupation of 12.4% for 144 h+ and 5.2% for 144 h-, respectively. LC-MS/MS results as a direct proteomic mapping approach reveal more daptomycin synthetic and regulation-related proteins from precursor group in terms of methyltransferase, ATP-binding cassette (ABC) transporters, resistance proteins and regulators.
authors: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors: Chiming
authors: C
authors: Ye
authors: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; The Key Laboratory for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China. Electronic address: yswu@xmu.edu.cn.
authors: I-Son
authors: IS
authors: Ng
authors: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; The Key Laboratory for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China.
authors: Keju
authors: K
authors: Jing
authors: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; The Key Laboratory for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China.
authors: Yinghua
authors: Y
authors: Lu
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018533:Biomass; D002853:Chromatography, Liquid; D017576:Daptomycin / Q000096:biosynthesis* / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D005810:Multigene Family; D010084:Oxidation-Reduction; D040901:Proteomics / Q000379:methods*; D013302:Streptomyces / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D017576:Daptomycin
keywords: Daptomycin; Liquid chromatography tandem mass; Precursor; Proteomics; Streptomyces roseosporus