title: Evaluation of liquid chromatographic behavior of lumazinic derivatives, from α-dicarbonyl compounds, in different C18 columns: application to wine samples using a fused-core column and fluorescence detection.
abstract: Several C18 columns, packed with totally porous particles of different sizes and shell thicknesses, have been compared for simultaneous determination of α-dicarbonyl compounds, previous derivatization to lumazinic derivatives. Chromatographic conditions for the separation have been optimized for each column, and chromatographic parameters have been calculated and exhaustively compared. A core-shell C18 column provided the best results, and a HPLC method with fluorimetric detection has been proposed. The developed method has been validated in terms of linearity, precision, and sensitivity. Detection and quantification limits obtained were comprised between 0.02 and 0.30 and 0.07 and 1.0 ng mL(-1), respectively, while RSD values obtained were lower than 6% and 5% in intraday and interday repeatability studies, respectively. The method has been applied to analysis of the α-dicarbonyl compounds in different types of wines. The higher levels of the total α-dicarbonyl compounds were found in sweet wines and the lower levels in white wines.
authors: Department of Analytical Chemistry, University of Extremadura , 06006 Badajoz, Spain.
authors: María del Carmen
authors: Mdel C
authors: Hurtado-Sánchez
authors: Anunciación
authors: A
authors: Espinosa-Mansilla
authors: María Isabel
authors: MI
authors: Rodríguez-Cáceres
authors: Isabel
authors: I
authors: Durán-Merás
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005470:Fluorometry; D011621:Pteridines / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
chemical_list: D011621:Pteridines; C008297:lumazine
keywords: C18 columns; fluorescence detection; lumazinic derivatives; wine samples; α-dicarbonyl compounds