title: Oxidative stress in blood and testicle of rat following intraperitoneal administration of aluminum and indium.
abstract: Aluminum (Al) and indium (In) have embryotoxic, neurotoxic and genotoxic effects, oxidative stress being one of the possible mechanisms involved in their cytotoxicity. We have recently demonstrated that indium intraperitoneal (ip) administration induced histological disorganization of testicular tissue. In the present research we aimed at investigating the effect of Al and In ip administration on systemic and testicular oxidative stress status. Studies were performed on Wistar rats ip injected with Al, In or physiological solution for two weeks. Our results showed that In significantly decreased the absolute weight of testicles. Measurements of lactate dehydrogenase (LDH) and paraoxonase (PON) activities showed that In induced a significant augmentation in the first parameter but no changes were observed in the second. Both Al and In caused oxidative stress in testicles by increasing malondialdehyde (MDA) and protein carbonyls (PC) production. Concomitantly, thiol group (-SH) and glutathione (GSH) level were enhanced in the testicles. In the blood, while concentrations of MDA was not changed, those of GSH was significantly decreased in the Al and In groups. Our results indicated that Al and In cause oxidative stress both in blood and testicles but In has cytotoxic effect as well as negative impact on testicle weights. These findings could explain the testicular histological alterations previously described after In ip administration.
authors: University of Tunis El Manar Laboratory of Physiology, Faculty of Medicine of Tunis Tunis Tunisia.
authors: S
authors: S
authors: Maghraoui
authors: University of Medicine and Pharmacy "Iuliu Hatieganu" of Cluj-Napoca Department of Physiology 1-3 Clinicilor Street Cluj-Napoca Romania.
authors: Simona
authors: S
authors: Clichici
authors: University of Tunis El Manar Laboratory of Physiology, Faculty of Medicine of Tunis Tunis Tunisia.
authors: A
authors: A
authors: Ayadi
authors: University of Medicine and Pharmacy "Iuliu Hatieganu" of Cluj-Napoca Department of Physiology 1-3 Clinicilor Street Cluj-Napoca Romania.
authors: C
authors: C
authors: Login
authors: University of Medicine and Pharmacy "Iuliu Hatieganu" of Cluj-Napoca Department of Physiology 1-3 Clinicilor Street Cluj-Napoca Romania.
authors: R
authors: R
authors: Moldovan
authors: University of Medicine and Pharmacy "Iuliu Hatieganu" of Cluj-Napoca Department of Physiology 1-3 Clinicilor Street Cluj-Napoca Romania.
authors: D
authors: D
authors: Daicoviciu
authors: University of Medicine and Pharmacy "Iuliu Hatieganu" of Cluj-Napoca Department of Physiology 1-3 Clinicilor Street Cluj-Napoca Romania.
authors: N
authors: N
authors: Decea
authors: University of Medicine and Pharmacy "Iuliu Hatieganu" of Cluj-Napoca Department of Physiology 1-3 Clinicilor Street Cluj-Napoca Romania.
authors: A
authors: A
authors: Mureşan
authors: University of Tunis El Manar Laboratory of Physiology, Faculty of Medicine of Tunis Tunis Tunisia.
authors: L
authors: L
authors: Tekaya
mesh_terms: D017607:Aluminum Compounds / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D043303:Aryldialkylphosphatase / Q000097:blood; D015415:Biomarkers / Q000097:blood; D005978:Glutathione / Q000097:blood; D007204:Indium / Q000008:administration & dosage / Q000633:toxicity*; D007274:Injections, Intraperitoneal; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009566:Nitrates / Q000008:administration & dosage / Q000633:toxicity*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D009929:Organ Size; D018384:Oxidative Stress / Q000187:drug effects*; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
chemical_list: D017607:Aluminum Compounds; D015415:Biomarkers; D009566:Nitrates; D007204:Indium; D009569:Nitric Oxide; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D043303:Aryldialkylphosphatase; D005978:Glutathione; C050609:aluminum nitrate; C089767:indium nitrate
keywords: absolute weight; aluminum; blood; cytotoxicity; indium; oxidative stress; reactive oxygen species; testicles