title: Long-term stem cell labeling by collagen-functionalized single-walled carbon nanotubes.
abstract: The monitoring of grafted stem cells is crucial to assess the efficiency, effectiveness and safety of such stem cell-based therapies. In this regard, a reliable and cytocompatible labeling method for stem cells is critically needed. In this study, the collagen-functionalized single-walled carbon nanotubes (Col-SWCNTs) were used as imaging probes for labeling of human mesenchymal stem cells (hMSCs) and the inherent Raman scattering of SWCNTs was used to image the SWCNT-labeled cells. The results showed that the Col-SWCNTs exhibit efficient cellular internalization by hMSCs without affecting their proliferation and differentiation. The prolonged dwell time of Col-SWCNTs in cells ensured the long-term labeling for up to 2 weeks. This work reveals the potential of Col-SWCNTs as probes for long-term stem cell labeling.
authors: Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. Guoping.CHEN@nims.go.jp.
authors: Hongli
authors: H
authors: Mao
authors: Rong
authors: R
authors: Cai
authors: Naoki
authors: N
authors: Kawazoe
authors: Guoping
authors: G
authors: Chen
mesh_terms: D050156:Adipogenesis / Q000187:drug effects; D000469:Alkaline Phosphatase / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology; D002118:Calcium / Q000737:chemistry; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D036103:Nanotechnology; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010012:Osteogenesis / Q000187:drug effects; D013059:Spectrum Analysis, Raman; D013234:Stem Cells / Q000166:cytology*; D013499:Surface Properties
chemical_list: D037742:Nanotubes, Carbon; D003094:Collagen; D000469:Alkaline Phosphatase; D002118:Calcium