title: Association between Thrombophilia and Repeated Assisted Reproductive Technology Failures.
abstract: OBJECTIVE
  This study was performed to investigate the incidence of thrombophilic gene mutations in repeated assisted reproductive technology (ART) failures.
  
  
  METHODS
  The prevalence of mutated genes in the patients with a history of three or more previous ART failures was compared with the patients with a history of successful pregnancy following ARTs. The study group included 70 patients, 34 with three or more previously failed ARTs (A) and control group consisted of 36 patients with successful pregnancy following ARTs (B). All patients were tested for the presence of mutated thrombophilic genes including factor V Leiden (FVL), Methylenetetrahydrofolate reductase (MTHFR) and Prothrombin (G20210A) using real-time polymerase chain reaction (RT- PCR).
  
  
  RESULTS
  Mutation of FVL gene was detected in 5.9% women of group A (2 of 34) compared with 2.8% women (1 of 36) of control group (P = 0.6). Mutation of MTHFR gene was found in 35.3% (12 cases) as compared with 50% (18 cases) of control (35.3% versus 50%; P = 0.23). Regarding Prothrombin, only control group had 5.6% mutation (P = 0.49). No significant differences were detected in the incidences of FVL, Prothrombin and MTHFR in the study group A compared with the control group B.
  
  
  CONCLUSIONS
  The obtained results suggest that thrombophilia does not have a significant effect in ART failures.
authors: Women's Reproductive Health Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Kobra
authors: K
authors: Hamdi
authors: Maryam
authors: M
authors: Vaezi
authors: Behrooz
authors: B
authors: Dagigazar
authors: Mahzad
authors: M
authors: Mehrzad Sadagiani
authors: Laya
authors: L
authors: Farzadi
authors: Maryam
authors: M
authors: Pashaei-Asl
keywords: ART; Mutation; Pregnancy; Thrombophilia