title: Enantioseparation and selective detection of D-amino acids by ultra-high-performance liquid chromatography/mass spectrometry in analysis of complex biological samples.
abstract: The growing scientific attention in the biological function of D-amino acids leads to an increasing analytical interest for enantiomeric amino acid separation, which is still very challenging due to the lack of sufficiently sensitive, high-throughput analytical methods that can cope with often occurring matrix interferences and very low D-amino acid concentrations. Here, enantioseparation can benefit from improved resolution and chromatographic speed offered by modern UHPLC techniques and the precision of MS detection. We developed a RP-UHPLC-QqToF-MS method using pre-column OPA/IBLC derivatization for very precise discrimination of amino acids enantiomers. The method shows a superb sensitivity with limits of detection in the range of several pmol/l. It has neither shown matrix inferences in the tested very complex biological matrices (serum, plasma, urine and gut) nor stability or racemization problems.
authors: Research Unit Analytical BioGeoChemistry, Helmholtz Center Munich, Neuherberg, Germany.
authors: Constanze
authors: C
authors: Müller
authors: Research Unit Analytical BioGeoChemistry, Helmholtz Center Munich, Neuherberg, Germany.
authors: Juliano R
authors: JR
authors: Fonseca
authors: Research Unit Analytical BioGeoChemistry, Helmholtz Center Munich, Neuherberg, Germany.
authors: Theresa M
authors: TM
authors: Rock
authors: Comprehensive Pneumology Center (CPC), Ludwig-Maximilians University, Asklepios Clinic Gauting and Helmholtz Center Munich, Germany; German Center for Lung Research (DZL), Germany.
authors: Susanne
authors: S
authors: Krauss-Etschmann
authors: Research Unit Analytical BioGeoChemistry, Helmholtz Center Munich, Neuherberg, Germany; Analytical Food Chemistry, Technical University Munich, Germany. Electronic address: schmitt-kopplin@helmholtz-muenchen.de.
authors: Philippe
authors: P
authors: Schmitt-Kopplin
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007422:Intestines / Q000737:chemistry; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids
keywords: Chiral derivatization; Chiral separation; Complex matrices; Liquid chromatography; Mass spectrometry; OPA-IBLC