title: Clinical metabolomics and hematic ADMA predict the future onset of cardiorenal syndrome in young grown-up subjects who were born preterm.
abstract: OBJECTIVE
  To look for differences in the urinary metabolic profile and in the hematic asymmetric dimethylarginine (ADMA) levels between a group of young adults born preterm with an extremely low birthweight (<1000 g; ex-ELBW; n=19) and a control group of subjects born at term with a weight appropriate for their gestational age (AGA; n=13); and to look for a possible correlation between the urinary metabolic profile in ex-ELBW and their hematic levels of ADMA.
  
  
  METHODS
  Urine samples were analyzed by (1)H nuclear magnetic resonance spectroscopy, and then submitted to unsupervised and supervised multivariate analysis. Samples of blood were collected and ADMA concentration was assessed by high-performance liquid chromatography.
  
  
  RESULTS
  Using supervised PLS-DA (partial least squares discriminant analysis) model, the authors were able to discriminate between ex-ELBW and AGA. Statistically significant differences were detected in the ADMA levels between ex-ELBW and AGA (p<0.02). Ex-ELBW metabolic profile correlated with ADMA concentrations (r=0.456, p<0.05). Conversely, ADMA levels in AGA did not correlated with their metabolic profiles.
  
  
  CONCLUSIONS
  This study demonstrates the relevance of the metabolomic technique as a predictive tool of the metabolic status in ex-ELBW. The relationship between ex-ELBW urinary metabolic profile and their blood ADMA levels suggests the presence of a subclinical cardio-renal involvement in these subjects.
authors: Department of Medical Sciences "M. Aresu", University of Cagliari, Cagliari, Italy. Electronic address: piercard@inwind.it.
authors: P P
authors: PP
authors: Bassareo
authors: Department of Surgery, Section of Neonatal Intensive Care Unit and Puericulture, University of Cagliari, Cagliari, Italy.
authors: V
authors: V
authors: Fanos
authors: Department of Surgery, Section of Neonatal Intensive Care Unit and Puericulture, University of Cagliari, Cagliari, Italy.
authors: A
authors: A
authors: Noto
authors: Movement Disorders Center, Institute of Neurology, University of Cagliari, Cagliari, Italy.
authors: P
authors: P
authors: Solla
authors: Department of Surgery, Section of Neonatal Intensive Care Unit and Puericulture, University of Cagliari, Cagliari, Italy.
authors: L
authors: L
authors: Barberini
authors: Department of Medical Sciences "M. Aresu", University of Cagliari, Cagliari, Italy.
authors: G
authors: G
authors: Flore
authors: Department of Surgery, Section of Neonatal Intensive Care Unit and Puericulture, University of Cagliari, Cagliari, Italy.
authors: M
authors: M
authors: Puddu
authors: Department of Medical Sciences "M. Aresu", University of Cagliari, Cagliari, Italy.
authors: G
authors: G
authors: Mercuro
mesh_terms: D000328:Adult; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D059347:Cardio-Renal Syndrome / Q000097:blood*; D016022:Case-Control Studies; D016002:Discriminant Analysis; D005260:Female; D005865:Gestational Age; D006801:Humans; D052577:Infant, Extremely Low Birth Weight; D007231:Infant, Newborn; D016018:Least-Squares Analysis; D008297:Male; D055432:Metabolomics; D047928:Premature Birth / Q000097:blood*; D055815:Young Adult
chemical_list: C018524:N,N-dimethylarginine; D001120:Arginine
keywords: Asymmetric dimethylarginine; Cardio-renal syndrome; Heart; Kidney; Metabolomics; Prematurity