title: Acaricidal activity of essential oil from Lippia sidoides on unengorged larvae and nymphs of Rhipicephalus sanguineus (Acari: Ixodidae) and Amblyomma cajennense (Acari: Ixodidae).
abstract: The aims of this work were to identify the compounds and to investigate the acaricidal activity of the essential oil of Lippia sidoides for unengorged larvae and nymphs of Rhipicephalus sanguineus and Amblyomma cajennense. The oil was analyzed by gas chromatography and gas chromatography/mass spectrometry. In total, 22 compounds comprising 98.5% of the total peak area were identified. The major constituent of the essential oil was thymol (69.9%). The acaricidal activity against larvae and nymphs was assessed using a modified larval packet test. In all experiments, oils were tested at concentrations of 2.35, 4.70, 9.40 14.10 and 18.80 mg/mL. The mortalities of larvae and nymphs of R. sanguineus were 20.6, 47.8, 73.6, 99.5 and 99.0% and 12.0, 50.0, 76.3, 96.0 and 96.1%, respectively. For larvae and nymphs of A. cajennense the rates of mortality were 41.9, 63.3, 77.8, 82.5 and 100.0% and 0.0, 32.8, 64.8, 71.1 and 94.0%, respectively. The LC 90 values of the L. sidoides oil were 11.56 and 12.97 mg/mL for larvae and nymphs of R. sanguineus and 15.70 and 18.52 mg/mL for larvae and nymphs of A. cajennense, respectively. The essential oil from L. sidoides has acaricidal activity on unengorged larvae and nymphs of R. sanguineus and A. cajennense.
authors: Departamento de Química, Instituto de Ciências Exatas, Universidade Federal Rural do Rio de Janeiro, 23890-000, Seropédica-RJ, Brazil.
authors: Geovany Amorim
authors: GA
authors: Gomes
authors: Programa de Pós-graduação em Ciências Veterinárias, Universidade Federal Rural do Rio de Janeiro, 23890-000, Seropédica-RJ, Brazil.
authors: Caio Márcio Oliveira
authors: CM
authors: Monteiro
authors: Departamento de Química, Instituto de Ciências Exatas, Universidade Federal Rural do Rio de Janeiro, 23890-000, Seropédica-RJ, Brazil.
authors: Lisieux de Santana
authors: Lde S
authors: Julião
authors: Programa de Pós-graduação em Ciências Veterinárias, Universidade Federal Rural do Rio de Janeiro, 23890-000, Seropédica-RJ, Brazil. Electronic address: ralphmaturano@gmail.com.
authors: Ralph
authors: R
authors: Maturano
authors: Programa de Pós-graduação em Ciências Biológicas, Comportamento e Biologia Animal da Universidade Federal de Juiz de Fora, 36036-900, Juiz de Fora-MG, Brazil.
authors: Tatiane Oliveira Souza
authors: TO
authors: Senra
authors: Programa de Pós-graduação em Ciências Veterinárias, Universidade Federal Rural do Rio de Janeiro, 23890-000, Seropédica-RJ, Brazil.
authors: Viviane
authors: V
authors: Zeringóta
authors: Programa de Pós-graduação em Ciências Biológicas, Comportamento e Biologia Animal da Universidade Federal de Juiz de Fora, 36036-900, Juiz de Fora-MG, Brazil.
authors: Fernanda
authors: F
authors: Calmon
authors: Programa de Pós-graduação em Ciências Biológicas, Comportamento e Biologia Animal da Universidade Federal de Juiz de Fora, 36036-900, Juiz de Fora-MG, Brazil.
authors: Renata da Silva
authors: Rda S
authors: Matos
authors: Programa de Pós-graduação em Ciências Biológicas, Comportamento e Biologia Animal da Universidade Federal de Juiz de Fora, 36036-900, Juiz de Fora-MG, Brazil.
authors: Erik
authors: E
authors: Daemon
authors: Departamento de Química, Instituto de Ciências Exatas, Universidade Federal Rural do Rio de Janeiro, 23890-000, Seropédica-RJ, Brazil.
authors: Mario Geraldo de
authors: MG
authors: Carvalho
mesh_terms: D056810:Acaricides; D000818:Animals; D016001:Confidence Intervals; D026863:Ixodidae; D007814:Larva; D032411:Lippia / Q000737:chemistry*; D009758:Nymph; D009822:Oils, Volatile / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry; D048508:Rhipicephalus sanguineus; D018709:Statistics, Nonparametric; D013943:Thymol / Q000032:analysis
chemical_list: D056810:Acaricides; D009822:Oils, Volatile; D010938:Plant Oils; D013943:Thymol
keywords: Brown dog tick; Cayenne tick; Thymol