title: Screening of biomarkers for lung cancer with gene expression profiling data.
abstract: OBJECTIVE
  Lung cancer is one of the most common malignant tumors, but the etiology is not yet clear. Our study aims to deepen the understandings about the mechanisms of lung cancer via screening relevant key genes and functional pathways.
  
  
  METHODS
  Microarray data set was collected and differentially expressed genes (DEGs) were selected out. KEGG pathway analysis and Gene Ontology (GO) enrichment analysis were performed for the DEGs. Interaction networks were constructed for the lung cancer-related DEGs with information from Human Protein Reference Database (HRPD) to screen out potential biomarkers.
  
  
  RESULTS
  Functional annotation revealed that cell cycle, DNA replication, immune system, and signal molecules and interactions were significantly over-represented in all the DEGs, suggesting their close involvement in the development of lung cancer. 40 genes with high degree, betweenness and clustering coefficient were identified from the interaction network. 26 out of them are known cancer genes according to the database F-census. Besides, 4 biomarkers were revealed through analyzing their interactions with oncogenes.
  
  
  CONCLUSIONS
  Our study not only advances the understandings about the molecular mechanisms of lung cancer, but also provides several potential biomarkers for clinical use.
authors: Department of Thoracic Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing, China. jiaxinminxq@hotmail.com.
authors: Q-P
authors: QP
authors: Wu
authors: J-X
authors: JX
authors: Min
authors: L
authors: L
authors: Jiang
authors: J-M
authors: JM
authors: Li
authors: K
authors: K
authors: Yao
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D016000:Cluster Analysis; D019295:Computational Biology; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D020022:Genetic Predisposition to Disease; D005820:Genetic Testing / Q000379:methods; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D011237:Predictive Value of Tests; D060066:Protein Interaction Maps
chemical_list: D014408:Biomarkers, Tumor