title: The neuroprotective effect of immune serum of adeno-associated virus vaccine containing Aβ1-15 gene on amyloid toxicity.
abstract: OBJECTIVE
  The aim of this study was to explore the effect of adeno-associated virus (AAV) serotype 2 vector vaccine containing amyloid-β peptide (Aβ) 1-15 gene fragment (AAV-Aβ15) immunized mice sera on counteracting Aβ1-42 peptide toxicity towards a primary culture cortical neurons.
  
  
  METHODS
  BALB/c mice were vaccinated via the intramuscular immunization route with AAV-Aβ15. The anti-Aβ antibody titer of immunized mice sera was quantified by sandwich Enzyme-Linked ImmunoSorbent Assay. The toxicity of Aβ1-42 peptide on neurons was assessed by morphology with an inverse microscopy and cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.
  
  
  RESULTS
  AAV-Aβ15 could induce an Aβ-specific immunoglobulin G (IgG) humoral immune response in /c mice the anti-Aβ antibodies were detectable at 1 month after immunization, significantly increased at 2 and 4 months after immunization, and the immunized sera could attenuate cytotoxicity of Aβ1-42 peptide on primary culture cortical neurons.
  
  
  CONCLUSIONS
  The immune serum of AAV-Aβ15 could play a neuroprotective effect against Aβ1-42 peptide toxicity, which would be beneficial for Alzheimer's disease patients.
authors: Department of Neurology, Yangpu Central Hospital, Shanghai, People's Republic of China.
authors: Ling-Yun
authors: LY
authors: Liu
authors: Yuan-Yuan
authors: YY
authors: Ma
authors: Tao
authors: T
authors: Yang
authors: Xin
authors: X
authors: Li
authors: Wen
authors: W
authors: Li
keywords: Alzheimer's disease; amyloid-beta; immunotherapy; vaccine