title: Real-time evaluation of aggregation using confocal imaging and image analysis tools.
abstract: Real-time confocal imaging was utilised to monitor the in situ loss of BSA monomers and aggregate formation using Spatial Intensity Distribution Analysis (SpIDA) and Raster Image Correlation Spectroscopy (RICS). At the proof of concept level this work has demonstrated the applicability of RICS and SpIDA for monitoring protein oligomerisation and larger aggregate formation.
authors: School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Manchester, UK. zahra.hamrang@manchester.ac.uk alain.pluen@manchester.ac.uk.
authors: Zahra
authors: Z
authors: Hamrang
authors: Egor
authors: E
authors: Zindy
authors: David
authors: D
authors: Clarke
authors: Alain
authors: A
authors: Pluen
mesh_terms: D000818:Animals; D002417:Cattle; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013997:Time Factors
chemical_list: D012710:Serum Albumin, Bovine