title: Effect of heat stress on the expression profile of Hsp90 among Sahiwal (Bos indicus) and Frieswal (Bos indicus × Bos taurus) breed of cattle: a comparative study.
abstract: We evaluated the effect of thermal challenge on the expression profile of heat shock protein 90 (Hsp90) among Sahiwal (Bos indicus) and Frieswal (Bos indicus × Bos taurus) breeds of cattle. The present investigation was focused on the comparative studies on Hsp90 expression among Frieswal and Sahiwal under in vitro and environmental heat stress. Measured immediately after the in vitro heat shock to the peripheral blood mononuclear cells (PBMCs), the relative expression of Hsp90 mRNA was significantly (P<0.05) higher in Sahiwal compared to those in Frieswal. In later intervals of time, the differences in the expression levels between the two breeds become negligible coming down towards the basal level. A similar pattern was observed in the protein concentration showing significantly (P<0.05) higher levels in Sahiwal compared to those in Frieswal. The second sets of experiments were undertaken during summer months (March to May) when temperature peaked from 37 to 45 °C. During these months, Frieswal cows consistently recorded higher rectal temperatures than the Sahiwal breed. Further during this peak summer stress, Sahiwal showed significantly higher levels of mRNA transcripts as well as protein concentration compared to the Frieswal breed. Our findings also interestingly showed that, the cell viability of PBMC are significantly higher among the Sahiwal than Frieswal. Taken together, the experiments of both induced in vitro and environmental stress conditions indicate that, Sahiwal may express higher levels of Hsp90 then Frieswal to regulate their body temperature and increase cell survivality under heat stressed conditions.
authors: Molecular Genetics Laboratory, Animal Genetics & Breeding Section, Project Directorate on Cattle, Indian Council of Agricultural Research, Grass Farm Road, P. O. Box 17, Meerut Cantt., Meerut 250 001, Uttar Pradesh, India. Electronic address: drrajibdeb@gmail.com.
authors: Rajib
authors: R
authors: Deb
authors: Molecular Genetics Laboratory, Animal Genetics & Breeding Section, Project Directorate on Cattle, Indian Council of Agricultural Research, Grass Farm Road, P. O. Box 17, Meerut Cantt., Meerut 250 001, Uttar Pradesh, India.
authors: Basavaraj
authors: B
authors: Sajjanar
authors: Molecular Genetics Laboratory, Animal Genetics & Breeding Section, Project Directorate on Cattle, Indian Council of Agricultural Research, Grass Farm Road, P. O. Box 17, Meerut Cantt., Meerut 250 001, Uttar Pradesh, India.
authors: Umesh
authors: U
authors: Singh
authors: Molecular Genetics Laboratory, Animal Genetics & Breeding Section, Project Directorate on Cattle, Indian Council of Agricultural Research, Grass Farm Road, P. O. Box 17, Meerut Cantt., Meerut 250 001, Uttar Pradesh, India.
authors: Sushil
authors: S
authors: Kumar
authors: Molecular Genetics Laboratory, Animal Genetics & Breeding Section, Project Directorate on Cattle, Indian Council of Agricultural Research, Grass Farm Road, P. O. Box 17, Meerut Cantt., Meerut 250 001, Uttar Pradesh, India.
authors: Rani
authors: R
authors: Singh
authors: Molecular Genetics Laboratory, Animal Genetics & Breeding Section, Project Directorate on Cattle, Indian Council of Agricultural Research, Grass Farm Road, P. O. Box 17, Meerut Cantt., Meerut 250 001, Uttar Pradesh, India.
authors: G
authors: G
authors: Sengar
authors: Molecular Genetics Laboratory, Animal Genetics & Breeding Section, Project Directorate on Cattle, Indian Council of Agricultural Research, Grass Farm Road, P. O. Box 17, Meerut Cantt., Meerut 250 001, Uttar Pradesh, India.
authors: Arjava
authors: A
authors: Sharma
mesh_terms: D000818:Animals; D001947:Breeding; D002417:Cattle; D002470:Cell Survival / Q000235:genetics; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics*; D018869:Heat-Shock Response / Q000235:genetics*; D006358:Hot Temperature; D007963:Leukocytes, Mononuclear; D012333:RNA, Messenger / Q000235:genetics; D059467:Transcriptome / Q000235:genetics*
chemical_list: D018841:HSP90 Heat-Shock Proteins; D012333:RNA, Messenger
keywords: ANOVA; ELISA; Expression; Frieswal; HF; Heat shock protein 90; Heat stress; Holstein Friesian; Hsp; PBMC; Sahiwal; analysis of variants; enzyme linked immune sorbent assay; heat shock protein; peripheral blood mononuclear cells; qPCR; quantative polymerase chain reaction