title: [Hematological indices of rats having complete and vitamin-deficient diets enriched with dietary fibers].
abstract: The hematological indices in 48 rats Wistar male with initial body weight 58.1+/- 0.5g has been studied. The rats were divided into 6 group and fed the complete semi-synthetic diet, containing 100% or 20% of vitamin mixture (Vit) with or without addition of dietary fiber (DF) in the form of wheat bran (5% of diet mass) during 4 weeks. The animals of the 1 group received 100% of vitamin mixture (100% Vit); of the 2 group--100% Vit+DF; 3 group--20% of vitamin mixture (20% Vit); 4 group--20% of vitamin mixture and DF (20% Vit+DF). The next 5 days rats from vitamin-deficient groups were fed with diets supplemented with 80% of Vit: (5 group--20% Vit+ 80% Vit; 6 group--20% Vit+DF+80% Vit). The animals fed vitamin-deficient diet lag significantly in growth from animals fed a complete diet. The growth curve of rats fed a diet with DF took an intermediate position. The studies were carried out at the Hematology analyzer "Coulter AC TTM 5 diff OV" (Beckman Coulter, USA) under the program, developed for the study of rat blood. Mean corpuscular volume (MCV) and mean corpuscular hemoglobin content (MCHC) were significantly decreased in rats with combined vitamin deficiency (20% Vit) in the diet compared with those of control group rats, while the compensatory increase in the number of red blood cells (RBC) and leukocytopenia took place. The enrichment of vitamin-deficient diet with DF (20% Vit+DF) prevented MCV and RBC changes, but MCHC left reduced in comparison with the indicator of the rats in control group. Indicators characterizing the state of platelets had no statistically significant differences between the groups. Compensation of vitamin deficiency in the diet of rats from group 5 (20% Vit+80% Vit) led only to the normalization of MCV. After vitamin restore in the diet of group 6 (20% Vit+DF+80% Vit) all investigated parameters were indistinguishable from the parameters of the control group. This indicates a positive effect of wheat bran DF consumption on the studied hematological indices.
authors: O K
authors: OK
authors: Mustafina
authors: É N
authors: ÉN
authors: Trushina
authors: O V
authors: OV
authors: Kosheleva
authors: O G
authors: OG
authors: Pereverzeva
authors: V M
authors: VM
authors: Kodentsova
authors: O A
authors: OA
authors: Vrzhesinskaia
mesh_terms: D000818:Animals; D001361:Avitaminosis / Q000097:blood* / Q000178:diet therapy; D004043:Dietary Fiber / Q000494:pharmacology*; D004906:Erythrocyte Count; D006454:Hemoglobins / Q000378:metabolism*; D007970:Leukopenia / Q000097:blood* / Q000178:diet therapy; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014815:Vitamins / Q000494:pharmacology*
chemical_list: D004043:Dietary Fiber; D006454:Hemoglobins; D014815:Vitamins