title: Antibiofilm activity of a monolayer of silver nanoparticles anchored to an amino-silanized glass surface.
abstract: Biofilm production is the crucial pathogenic mechanism of the implant-associated infection and a primary target for new anti-infective strategies. Silver nanoparticles (AgNPs) are attracting interest for their multifaceted potential biomedical applications. As endowed with highest surface/mass ratio and potent antibacterial activity, they can profitably be applied as monolayers at biomaterial surfaces. Desirably, in order to minimize the risks of toxic effects from freely circulating detached nanoparticles, AgNPs should firmly be anchored to the modified biomaterial surfaces. Here we focus on a newly designed glass surface modified with AgNPs and on its antibiofilm properties. Link of a self-assembled monolayer of AgNPs to glass was obtained through preliminary amino-silanization of the glass followed by immersion in an AgNPs colloidal suspension. Static contact angle measure, AFM, TEM, UV-Vis spectroscopy, ICP atomic emission spectroscopy were used for characterization. Antibiofilm activity against the biofilm-producer Staphylococcus epidermidis RP62A was assayed by both CFU method and CLSM. Performances of AgNPs-glasses were: i) excellent stability in aqueous medium; ii) prolonged release and high local concentration of Ag(+) without any detaching of AgNPs; iii) strong antibiofilm activity against S. epidermidis RP62A. This AgNPs surface-modification can be applied to a large variety of biomaterials by simply depositing glass-like SiO2 films on their surfaces.
authors: Inorganic Nanochemistry Laboratory, Department of Chemistry, University of Pavia, Pavia, Italy.
authors: Angelo
authors: A
authors: Taglietti
authors: Research Unit on Implant Infections, Rizzoli Orthopaedic Institute, Bologna, Italy; DIMES, University of Bologna, Bologna, Italy. Electronic address: carlarenata.arciola@ior.it.
authors: Carla Renata
authors: CR
authors: Arciola
authors: Inorganic Nanochemistry Laboratory, Department of Chemistry, University of Pavia, Pavia, Italy.
authors: Agnese
authors: A
authors: D'Agostino
authors: Inorganic Nanochemistry Laboratory, Department of Chemistry, University of Pavia, Pavia, Italy.
authors: Giacomo
authors: G
authors: Dacarro
authors: Research Unit on Implant Infections, Rizzoli Orthopaedic Institute, Bologna, Italy; DIMES, University of Bologna, Bologna, Italy.
authors: Lucio
authors: L
authors: Montanaro
authors: Research Unit on Implant Infections, Rizzoli Orthopaedic Institute, Bologna, Italy.
authors: Davide
authors: D
authors: Campoccia
authors: Inorganic Nanochemistry Laboratory, Department of Chemistry, University of Pavia, Pavia, Italy.
authors: Lucia
authors: L
authors: Cucca
authors: Center for Tissue Engineering (C.I.T.), Department of Molecular Medicine, INSTM UdR of Pavia, University of Pavia, Pavia, Italy; Nanotechnology Laboratory, Department of Occupational Medicine, Ergonomy and Disability, Salvatore Maugeri Foundation, IRCCS, Pavia, Italy.
authors: Marco
authors: M
authors: Vercellino
authors: Pediatrics Unit, Department of Mother and Child, University of Modena and Reggio Emilia, Modena, Italy.
authors: Alessandro
authors: A
authors: Poggi
authors: Inorganic Nanochemistry Laboratory, Department of Chemistry, University of Pavia, Pavia, Italy.
authors: Piersandro
authors: P
authors: Pallavicini
authors: Center for Tissue Engineering (C.I.T.), Department of Molecular Medicine, INSTM UdR of Pavia, University of Pavia, Pavia, Italy; Nanotechnology Laboratory, Department of Occupational Medicine, Ergonomy and Disability, Salvatore Maugeri Foundation, IRCCS, Pavia, Italy.
authors: Livia
authors: L
authors: Visai
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects; D005898:Glass / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology*; D013212:Staphylococcus epidermidis / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D012834:Silver
keywords: APTES; Anti-infective biomaterials; Biofilm; Silver nanoparticles; Staphylococcus epidermidis