title: Three dimensional visualization of engineered bone and soft tissue by combined x-ray micro-diffraction and phase contrast tomography.
abstract: Computed x-ray phase contrast micro-tomography is the most valuable tool for a three dimensional (3D) and non destructive analysis of the tissue engineered bone morphology. We used a Talbot interferometer installed at SYRMEP beamline of the ELETTRA synchrotron (Trieste, Italy) for a precise 3D reconstruction of both bone and soft connective tissue, regenerated in vivo within a porous scaffold. For the first time the x-ray tomographic reconstructions have been combined with x-ray scanning micro-diffraction measurement on the same sample, in order to give an exhaustive identification of the different tissues participating to the biomineralization process. As a result, we were able to investigate in detail the different densities in the tissues, distinguishing the 3D organization of the amorphous calcium phosphate from the collagen matrix. Our experimental approach allows for a deeper understanding of the role of collagen matrix in the organic-mineral transition, which is a crucial issue for the development of new bio-inspired composites.
authors: Istituto di Fotonica e Nanotecnologie - CNR, via Cineto Romano 42, I-00156 Roma, Italy.
authors: Alessia
authors: A
authors: Cedola
authors: Gaetano
authors: G
authors: Campi
authors: Daniele
authors: D
authors: Pelliccia
authors: Inna
authors: I
authors: Bukreeva
authors: Michela
authors: M
authors: Fratini
authors: Manfred
authors: M
authors: Burghammer
authors: Luigi
authors: L
authors: Rigon
authors: Fulvia
authors: F
authors: Arfelli
authors: Rong
authors: R
authors: Chang Chen
authors: Diego
authors: D
authors: Dreossi
authors: Nicola
authors: N
authors: Sodini
authors: Sara
authors: S
authors: Mohammadi
authors: Giuliana
authors: G
authors: Tromba
authors: Ranieri
authors: R
authors: Cancedda
authors: Maddalena
authors: M
authors: Mastrogiacomo
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000000981:diagnostic imaging* / Q000502:physiology; D002113:Calcification, Physiologic; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007368:Interferometry; D051379:Mice; D011859:Radiography; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014054:Tomography; D014961:X-Ray Diffraction