title: Two-dimensional crystallization of intact F-ATP synthase isolated from bovine heart mitochondria.
abstract: Mitochondrial F-ATP synthase produces the majority of ATP for cellular functions requiring free energy. The structural basis for proton motive force-driven rotational catalysis of ATP formation in the holoenzyme remains to be determined. Here, the purification and two-dimensional crystallization of bovine heart mitochondrial F-ATP synthase are reported. Two-dimensional crystals of up to 1 µm in size were grown by dialysis-mediated detergent removal from a mixture of decylmaltoside-solubilized 1,2-dimyristoyl-sn-glycero-3-phosphocholine and F-ATP synthase against a detergent-free buffer. A projection map calculated from an electron micrograph of a negatively stained two-dimensional crystal revealed unit-cell parameters of a = 185.0, b = 170.3 Å, γ = 92.5°.
authors: Picobiology Institute, Graduate School of Life Science, University of Hyogo, 3-2-1 Kouto, Kamigori Akoh, Hyogo 678-1297, Japan.
authors: Shintaro
authors: S
authors: Maeda
authors: Kyoko
authors: K
authors: Shinzawa-Itoh
authors: Kaoru
authors: K
authors: Mieda
authors: Mami
authors: M
authors: Yamamoto
authors: Yumiko
authors: Y
authors: Nakashima
authors: Yumi
authors: Y
authors: Ogasawara
authors: Chimari
authors: C
authors: Jiko
authors: Kazutoshi
authors: K
authors: Tani
authors: Atsuo
authors: A
authors: Miyazawa
authors: Christoph
authors: C
authors: Gerle
authors: Shinya
authors: S
authors: Yoshikawa
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D003460:Crystallization; D018360:Crystallography, X-Ray; D004134:Dimyristoylphosphatidylcholine / Q000031:analogs & derivatives / Q000737:chemistry; D008854:Microscopy, Electron; D008929:Mitochondria, Heart / Q000737:chemistry* / Q000201:enzymology; D025261:Mitochondrial Proton-Translocating ATPases / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D021122:Protein Subunits / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: C108836:1,2-dimyristoyl-sn-glycero-3-ethylphosphocholine; D021122:Protein Subunits; D000255:Adenosine Triphosphate; D025261:Mitochondrial Proton-Translocating ATPases; D004134:Dimyristoylphosphatidylcholine
keywords: bovine F-ATP synthase