title: Anti-HIV-1 activity of Trim 37.
abstract: Trim 5α was the first member of the tripartite motif (TRIM) family of proteins that was identified to potently restrict human immunodeficiency virus type 1 (HIV-1) replication. The breadth of antiretroviral activity of TRIM family members is an active area of investigation. In this study, we demonstrate that human Trim 37 possesses anti-HIV-1 activity. This antiretroviral activity and the manner in which it was displayed were implicated by (1) decreased viral replication upon Trim 37 transient overexpression in virus-producing cells, (2) correlation of the reduction of viral infectivity with Trim 37 virion incorporation, (3) increased HIV-1 replication during siRNA depletion of Trim 37 expression, and (4) reduction in viral DNA synthesis upon Trim 37 transient overexpression. Our findings provide the first demonstration, to our knowledge, of the potent antiviral activity of human Trim 37, and implicate an antiviral mechanism whereby Trim 37 interferes with viral DNA synthesis.
authors: MinnCResT Program, Department of Diagnostic and Biological Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN 55455; USA.|Institute for Molecular Virology, University of Minnesota, Minneapolis, MN 55455; USA.
authors: Azah A
authors: AA
authors: Tabah
authors: Myriad Pharmaceuticals, Salt Lake City, UT 84108, USA.
authors: Keith
authors: K
authors: Tardif
authors: Department of Microbiology, University of Minnesota, Minneapolis, MN 55455; USA.|MinnCResT Program, Department of Diagnostic and Biological Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN 55455; USA.|Institute for Molecular Virology, University of Minnesota, Minneapolis, MN 55455; USA.
authors: Louis M
authors: LM
authors: Mansky
mesh_terms: D004279:DNA, Viral / Q000096:biosynthesis; D015497:HIV-1 / Q000276:immunology* / Q000502:physiology; D006801:Humans; D009687:Nuclear Proteins / Q000276:immunology* / Q000378:metabolism*; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D009687:Nuclear Proteins; D000070584:Tripartite Motif Proteins; C411745:TRIM37 protein, human; D044767:Ubiquitin-Protein Ligases