title: Endosalpingiosis as it relates to tubal, ovarian and serous neoplastic tissues: an immunohistochemical study of tubal and Müllerian antigens.
abstract: OBJECTIVE
  The origins and clinical significance of endosalpingiosis (ES), ectopic tubal epithelium, are not well understood. These investigations aim to characterize ES as it relates to normal fallopian tube, ovarian surface and serous neoplasms.
  
  
  METHODS
  A retrospective review of pathology reports from all prophylactic gynecologic surgeries from 2000 to 2010 was performed to assess the frequency of ES. Twenty-one archival specimens of ES, 6 normal fallopian tubes, 9 normal ovaries, 21 serous neoplasms and a commercially available ovarian tissue microarray were subjected to immunohistochemistry (IHC) with 11 tubal and Müllerian antigens. IHC staining was evaluated with a quantitative scoring system and scores were analyzed using MINITAB statistical software.
  
  
  RESULTS
  ES was noted in 3.5% of pathologic specimens from 464 prophylactic surgeries. The majority of antigens showed no significant differences (p > 0.05) in median IHC scores between ES and normal fallopian tube epithelium (nFTE), while they were significantly different (p < 0.05) from the ovarian surface epithelium (OSE). Median IHC scores were unchanged in ES tissues regardless of the location of ES or the presence of a concurrent serous neoplasm. Three antigens emerged as contemporary tubal and ES biomarkers: phospho-Smad2, BCL2 and FOXJ1. All 3 biomarkers were expressed in ES, nFTE and serous neoplasms, but not in OSE or other tumor types.
  
  
  CONCLUSIONS
  This study provides immunophenotypic evidence that ES is more similar to the nFTE than OSE. Further, ES biomarker expression closely resembles serous neoplasms strengthening the growing body of evidence that all Müllerian serous carcinomas arise from tubal-like epithelium.
authors: Laboratory of Gynecologic Oncology, Division of Gynecologic Oncology, Brigham & Women's Hospital, Harvard Medical School, Boston, MA, USA.
authors: Katharine M
authors: KM
authors: Esselen
authors: School of Medicine, Griffith Health Institute, Griffith University, Meadowbrook, QLD 4131, Australia.
authors: Shu-Kay
authors: SK
authors: Ng
authors: Laboratory of Gynecologic Oncology, Division of Gynecologic Oncology, Brigham & Women's Hospital, Harvard Medical School, Boston, MA, USA; Department of Obstetrics & Gynecology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, PR China.
authors: Yuanyuan
authors: Y
authors: Hua
authors: Laboratory of Gynecologic Oncology, Division of Gynecologic Oncology, Brigham & Women's Hospital, Harvard Medical School, Boston, MA, USA.
authors: Miranda
authors: M
authors: White
authors: Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
authors: Cynthia A
authors: CA
authors: Jimenez
authors: Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
authors: William R
authors: WR
authors: Welch
authors: Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA; Department of Medical Oncology, Harvard Medical School, Dana-Farber Cancer Institute, Boston, MA, USA.
authors: Ronny
authors: R
authors: Drapkin
authors: Laboratory of Gynecologic Oncology, Division of Gynecologic Oncology, Brigham & Women's Hospital, Harvard Medical School, Boston, MA, USA.
authors: Ross S
authors: RS
authors: Berkowitz
authors: Laboratory of Gynecologic Oncology, Division of Gynecologic Oncology, Brigham & Women's Hospital, Harvard Medical School, Boston, MA, USA. Electronic address: sng@partners.org.
authors: Shu-Wing
authors: SW
authors: Ng
mesh_terms: D000951:Antigens, Neoplasm / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D018284:Cystadenocarcinoma, Serous / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005184:Fallopian Tube Diseases / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D005185:Fallopian Tube Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D009095:Mullerian Ducts / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D011230:Precancerous Conditions / Q000378:metabolism / Q000473:pathology; D012189:Retrospective Studies
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor
keywords: Endosalpingiosis; Fallopian tube epithelium; Immunohistochemistry; Serous ovarian neoplasm