title: Challenges and opportunities in the purification of recombinant tagged proteins.
abstract: The purification of recombinant proteins by affinity chromatography is one of the most efficient strategies due to the high recovery yields and purity achieved. However, this is dependent on the availability of specific affinity adsorbents for each particular target protein. The diversity of proteins to be purified augments the complexity and number of specific affinity adsorbents needed, and therefore generic platforms for the purification of recombinant proteins are appealing strategies. This justifies why genetically encoded affinity tags became so popular for recombinant protein purification, as these systems only require specific ligands for the capture of the fusion protein through a pre-defined affinity tag tail. There is a wide range of available affinity pairs "tag-ligand" combining biological or structural affinity ligands with the respective binding tags. This review gives a general overview of the well-established "tag-ligand" systems available for fusion protein purification and also explores current unconventional strategies under development.
authors: REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal; IBET-Instituto de Biologia Experimental Tecnológica, Oeiras, Portugal.
authors: Ana Sofia
authors: AS
authors: Pina
authors: Institute of Biotechnology, Department of Chemical Engineering and Biotechnology, University of Cambridge, Tennis Court Road, CB2 1QT Cambridge, UK.
authors: Christopher R
authors: CR
authors: Lowe
authors: REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal. Electronic address: cecilia.roque@fct.unl.pt.
authors: Ana Cecília A
authors: AC
authors: Roque
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D006801:Humans; D008024:Ligands; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification*
chemical_list: D008024:Ligands; D011993:Recombinant Fusion Proteins
keywords: Affinity ligands; Affinity tags; Fusion proteins; Recombinant proteins