title: RBOH1-dependent H2O2 production and subsequent activation of MPK1/2 play an important role in acclimation-induced cross-tolerance in tomato.
abstract: H2O2 and mitogen-activated protein kinase (MAPK) cascades play important functions in plant stress responses, but their roles in acclimation response remain unclear. This study examined the functions of H2O2 and MPK1/2 in acclimation-induced cross-tolerance in tomato plants. Mild cold, paraquat, and drought as acclimation stimuli enhanced tolerance to more severe subsequent chilling, photooxidative, and drought stresses. Acclimation-induced cross-tolerance was associated with increased transcript levels of RBOH1 and stress- and defence-related genes, elevated apoplastic H2O2 accumulation, increased activity of NADPH oxidase and antioxidant enzymes, reduced glutathione redox state, and activation of MPK1/2 in tomato. Virus-induced gene silencing of RBOH1, MPK1, and MPK2 or MPK1/2 all compromised acclimation-induced cross-tolerance and associated stress responses. Taken together, these results strongly suggest that acclimation-induced cross-tolerance is largely attributed to RBOH1-dependent H2O2 production at the apoplast, which may subsequently activate MPK1/2 to induce stress responses.
authors: Department of Horticulture, Zijingang Campus, Zhejiang University, Yuhangtang Road 866, Hangzhou 310058, PR China.
authors: Jie
authors: J
authors: Zhou
authors: Xiao-Jian
authors: XJ
authors: Xia
authors: Yan-Hong
authors: YH
authors: Zhou
authors: Kai
authors: K
authors: Shi
authors: Zhixiang
authors: Z
authors: Chen
authors: Jing-Quan
authors: JQ
authors: Yu
mesh_terms: D000064:Acclimatization / Q000528:radiation effects; D000975:Antioxidants / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D003080:Cold Temperature; D004789:Enzyme Activation / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008027:Light; D018551:Solanum lycopersicum / Q000201:enzymology* / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics / Q000528:radiation effects
chemical_list: D000975:Antioxidants; D010940:Plant Proteins; D012333:RNA, Messenger; D002245:Carbon Dioxide; D006861:Hydrogen Peroxide; D020928:Mitogen-Activated Protein Kinases; D005978:Glutathione
keywords: Cross-tolerance; Respiratory burst oxidase homologue 1; Solanum lycopersicum.; hydrogen peroxide; mitogen-activated protein kinase; reactive oxygen species; signal transduction