title: Structural elucidation of the metabolites of lapachol in rats by liquid chromatography-tandem mass spectrometry.
abstract: Lapachol is a natural naphthoquinone compound derived from Bignoniaceae (Tabebuia sp.) that possesses a range of significant biological activities. Nine phase I and four phase II metabolites of lapachol in rat bile were firstly elucidated and identified using a sensitive LC-ESI-MS(n) method. The molecular structures of the metabolites have been presented on the basis of the characteristics of their precursor and product ions, as well as their fragmentation mechanisms and chromatographic retention times. The results indicated that the phase I metabolites were predominantly biotransformed by the hydroxylation, semiquinone hydrogenation at the oxygen position or a side chain rearrangement. The phase II metabolites were identified as the glucuronidated conjugates which showed a characteristic neutral loss of 176Da. Based on the results of this research, we have proposed the metabolic pathways for lapachol in rats. This work has provided novel information for the in vivo lapachol metabolism which could be used to develop a novel drug candidate, as well as a better understanding of the safety and efficacy of the drug.
authors: Department of Pharmacology, School of Basic Medical Sciences, Capital Medical Universit; Beijing Laboratory of Biomedical Detection and Instrument, Beijing 100069, China.
authors: Lu
authors: L
authors: Bai
authors: Department of Pharmacology, School of Basic Medical Sciences, Capital Medical Universit; Beijing Laboratory of Biomedical Detection and Instrument, Beijing 100069, China.
authors: Ying
authors: Y
authors: Han
authors: Department of Pharmacology, School of Basic Medical Sciences, Capital Medical Universit; Beijing Laboratory of Biomedical Detection and Instrument, Beijing 100069, China.
authors: Jinfeng
authors: J
authors: Yao
authors: Department of Pharmacology, School of Basic Medical Sciences, Capital Medical Universit; Beijing Laboratory of Biomedical Detection and Instrument, Beijing 100069, China.
authors: Xiaorong
authors: X
authors: Li
authors: Department of Pharmacology, School of Basic Medical Sciences, Capital Medical Universit; Beijing Laboratory of Biomedical Detection and Instrument, Beijing 100069, China.
authors: Yuhang
authors: Y
authors: Li
authors: Department of Pharmacology, School of Basic Medical Sciences, Capital Medical Universit; Beijing Laboratory of Biomedical Detection and Instrument, Beijing 100069, China.
authors: Pinxiang
authors: P
authors: Xu
authors: Department of Pharmacology, School of Basic Medical Sciences, Capital Medical Universit; Beijing Laboratory of Biomedical Detection and Instrument, Beijing 100069, China. Electronic address: xuem@ccum.edu.cn.
authors: Ming
authors: M
authors: Xue
mesh_terms: D000818:Animals; D001646:Bile / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D008297:Male; D008958:Models, Molecular; D009285:Naphthoquinones / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009285:Naphthoquinones; C008252:lapachol
keywords: LC-ESI–MS(n); Lapachol; Metabolites; Structural elucidation