title: Protein deficient chloroplast ribosomes in a yellow mutant of Chlamydomonas reinhardii.
abstract: Ribosomes and ribosomal proteins from wild-type and a yellow mutant of Chlamydomonas reinhardii were analyzed and compared by two-dimensional gel electrophoresis. The mixothrophically grown yellow-76 mutant differs from wild-type cells in lowered chlorophyll content and photosynthetic activity per chlorophyll unit. The latter is connected with the decreased activity of the ribulose-I,5-diphosphate-carboxylase enzyme. Analytical ultracentrifugation of cell extracts shows a normal amount of free 70S ribosomes and 50S subunit in the mutant cells. Two-dimensional gel electrophoresis shows considerable alterations in the protein composition of the 70S ribosomes of the mutant. Two proteins are absent from the electrophoretograms of the yellow-76 mutant, and seven proteins are present in reduced amounts. The genetical analysis shows a Mendelian pattern of inheritance, indicating that protein alterations presumably are localized in nuclear DNA.
authors: Department of Genetics, Eötvös Loránd University, Budapest, Hungary.
authors: I
authors: I
authors: Gyurján
authors: N P
authors: NP
authors: Yurina
authors: M S
authors: MS
authors: Turischeva
authors: M S
authors: MS
authors: Odintsova
authors: N N
authors: NN
authors: Alexandrova