title: Multiplex cytological profiling assay to measure diverse cellular states.
abstract: Computational methods for image-based profiling are under active development, but their success hinges on assays that can capture a wide range of phenotypes. We have developed a multiplex cytological profiling assay that "paints the cell" with as many fluorescent markers as possible without compromising our ability to extract rich, quantitative profiles in high throughput. The assay detects seven major cellular components. In a pilot screen of bioactive compounds, the assay detected a range of cellular phenotypes and it clustered compounds with similar annotated protein targets or chemical structure based on cytological profiles. The results demonstrate that the assay captures subtle patterns in the combination of morphological labels, thereby detecting the effects of chemical compounds even though their targets are not stained directly. This image-based assay provides an unbiased approach to characterize compound- and disease-associated cell states to support future probe discovery.
authors: Broad Institute of Harvard and MIT, Cambridge, Massachusetts, United States of America.
authors: Sigrun M
authors: SM
authors: Gustafsdottir
authors: Vebjorn
authors: V
authors: Ljosa
authors: Katherine L
authors: KL
authors: Sokolnicki
authors: J
authors: J
authors: Anthony Wilson
authors: Deepika
authors: D
authors: Walpita
authors: Melissa M
authors: MM
authors: Kemp
authors: Kathleen
authors: K
authors: Petri Seiler
authors: Hyman A
authors: HA
authors: Carrel
authors: Todd R
authors: TR
authors: Golub
authors: Stuart L
authors: SL
authors: Schreiber
authors: Paul A
authors: PA
authors: Clemons
authors: Anne E
authors: AE
authors: Carpenter
authors: Alykhan F
authors: AF
authors: Shamji
mesh_terms: D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007091:Image Processing, Computer-Assisted
chemical_list: D005456:Fluorescent Dyes