title: Acute hyperglycaemia does not alter nitric oxide-mediated microvascular function in the skin of adolescents with type 1 diabetes.
abstract: OBJECTIVE
  We assessed the impact of an acute bout of hyperglycaemia on nitric oxide (NO)-mediated microvascular function in the skin of adolescents with type 1 diabetes (T1DM).
  
  
  METHODS
  Twelve subjects (12-18 years) with T1DM were randomised into a control (n = 6) or hyperglycaemia (n = 6) group. Hyperinsulinaemic clamps were used to manipulate blood glucose level (BGL). Following a baseline period, where all subjects were euglycaemic (20 min), the experimental phase began. During the experimental phase, BGL was elevated to 16.7 ± 0.9 mmol L(-1) in the hyperglyceamic group, while it was maintained at euglycaemia (5.5 ± 0.1 mmol L(-1)) in the control group. Simultaneously, cutaneous microvascular function (% max cutaneous vascular conductance, CVC%) was assessed using laser Doppler fluxometry following stimulation of skin blood flow using localised heating (42 °C). To determine the NO contribution to skin blood flow, two microdialysis sites were assessed, one perfused with Ringers and the other with the NO blocker, NG-monomethyl-L-arginine (L-NMMA).
  
  
  RESULTS
  In the hyperglycaemic group, acute increase in BGL was not associated with changes in skin blood flow (CVC% 82.4 ± 8.7% at 5.5 ± 0.1 mmol L(-1) vs 79.5 ± 9.1% at 16.7 ± 0.9 mmol L(-1), unpaired t tests, P = 0.588) or the contribution of NO to vasodilation.
  
  
  CONCLUSIONS
  These results suggest that, in our group of adolescents with type 1 diabetes, acute hyperglycaemia did not affect skin microvascular NO-mediated function.
authors: School of Sport Science, Exercise and Health, The University of Western Australia, M408, 35 Stirling Highway, Crawley, WA, 6009, Australia, louise.naylor@uwa.edu.au.
authors: Louise H
authors: LH
authors: Naylor
authors: Norhaida M
authors: NM
authors: Yusof
authors: Nirubasini
authors: N
authors: Paramalingam
authors: Timothy W
authors: TW
authors: Jones
authors: Elizabeth A
authors: EA
authors: Davis
authors: Daniel J
authors: DJ
authors: Green
mesh_terms: D000293:Adolescent; D016022:Case-Control Studies; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism / Q000503:physiopathology*; D005260:Female; D006801:Humans; D006943:Hyperglycemia / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D055806:Microvessels / Q000378:metabolism / Q000503:physiopathology*; D009569:Nitric Oxide / Q000378:metabolism*; D012867:Skin / Q000098:blood supply*
chemical_list: D009569:Nitric Oxide