title: Immobilization of xylanase on poly (ethylene glycol) methyl ether 5000 and its self-extractive bioconversion for the production of xylo-oligosaccharides.
abstract: Endo-β-1,4-xylanase derived from Trichoderma reesei was covalently immobilized on poly (ethylene glycol) methyl ether 5000 (mPEG5000), and the resulting immobilized enzyme had a residual activity of 72.4 % with 82.9 % of PEGylated amino groups. Compared with the free enzyme, the immobilized xylanase was stable at pH values in the range of 4.0-6.0 and temperatures in the range of 50-65 °C. A self-extractive bioconversion system composed of immobilized xylanase, mPEG5000, and sodium citrate was used to produce xylo-oligosaccharides and provided a better distribution of the xylo-oligosaccharides than the free enzyme. Furthermore, the immobilized xylanase could be effectively recovered in situ following the hydrolysis reaction.
authors: Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, College of Chemical Engineering, Nanjing Forestry University, No. 159 Long Pan Road, Nanjing, 210037, Jiangsu Province, China.
authors: Xin
authors: X
authors: Li
authors: Zongxing
authors: Z
authors: Shan
authors: Xiangyang
authors: X
authors: Song
authors: Jia
authors: J
authors: Ouyang
authors: Yong
authors: Y
authors: Xu
authors: Qiang
authors: Q
authors: Yong
authors: Shiyuan
authors: S
authors: Yu
mesh_terms: D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000378:metabolism*; D014242:Trichoderma / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D009844:Oligosaccharides; D011092:Polyethylene Glycols; D043364:Endo-1,4-beta Xylanases