title: Mmu-miR-193 is involved in embryo implantation in mouse uterus by regulating GRB7 gene expression.
abstract: Embryo implantation is a complicated process involving a series of endometrial changes that depend on differential gene expression. MicroRNAs (miRNAs) are important for regulation of gene expression. Previous studies have shown that miRNAs may participate in the regulation of gene expression during embryo implantation. To explore the role of endometrial miRNAs in early murine pregnancy, we used microarrays to investigate whether miRNAs were differentially expressed in the mouse endometrium on pregnancy day 4 (D4) and day 6 (D6). The results demonstrated that 17 miRNAs were upregulated and 18 were downregulated (>2-fold) in D6 endometria compared to D4. We identified that mmu-miR-193 exhibited the highest upregulation on D6, and the upregulation of mmu-miR-193 before embryo implantation could reduce the embryo implantation rate. Further, we demonstrated that mmu-miR-193 influenced embryo implantation by regulating growth factor receptor-bound protein 7 expression. In summary, our study suggests that mmu-miR-193 plays an important role in embryo implantation.
authors: 1Laboratory of Reproduction Biology, Chongqing Medical University, Chongqing, China.
authors: Rong
authors: R
authors: Li
authors: Junlin
authors: J
authors: He
authors: Xuemei
authors: X
authors: Chen
authors: YuBin
authors: Y
authors: Ding
authors: Yingxiong
authors: Y
authors: Wang
authors: ChunLan
authors: C
authors: Long
authors: Lianju
authors: L
authors: Shen
authors: Xueqing
authors: X
authors: Liu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D010064:Embryo Implantation / Q000502:physiology*; D005260:Female; D051382:GRB7 Adaptor Protein / Q000096:biosynthesis*; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D035683:MicroRNAs / Q000502:physiology*; D011247:Pregnancy; D011897:Random Allocation; D014599:Uterus / Q000378:metabolism*
chemical_list: C495956:Grb7 protein, mouse; C568257:MIRN193 microRNA, mouse; D035683:MicroRNAs; D051382:GRB7 Adaptor Protein
keywords: GRB7; embryo implantation; microRNAs (miRNAs); mmu-miR-193