title: [Morphology and microRNA expression profiles of drug-resistant cells in hepatocellular carcinoma].
abstract: OBJECTIVE
  To compare morphological differences of three drug-resistant hepatocellular carcinoma (HCC) cell subclones (Huh-7/ADM, Huh-7/CBP, Huh-7/MMC) and their parental Huh-7 cell line, to analyze differential microRNA (miRNA) expression profiles in these cells and, finally to screen for the abnormal expressed miRNAs in drug-resistant HCC cells.
  
  
  METHODS
  Cellular morphology was observed by histology and transmission electron microscopy. MiRNA microarray was used to analyze the differential miRNA expression profiles in these cells (Huh-7, Huh-7/ADM, Huh-7/CBP, Huh-7/MMC) followed by real time quantitative PCR validation.
  
  
  RESULTS
  The drug-resistant cells had more intracytoplasmic organelles and were larger in size along with increased cytological pleomorphism than the parental Huh-7 cells. Compared with the parental Huh-7 cells, 32 simultaneously up-regulated and 22 down-regulated miRNAs were found in three drug-resistant cells. Up-regulation of miR-15a, miR-16, miR-27b, miR-30b, miR-146a, miR-146b-5p, miR-181a, miR-181d and miR-194 was verified by RT-qPCR.
  
  
  CONCLUSIONS
  Drug-resistant HCC cells have abnormal expressed miRNAs, which may be explored to further investigate the association of miRNA expressions with multidrugs resistance in HCC.
authors: Department of Pathology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350004, China.
authors: Li-juan
authors: LJ
authors: Zhuo
authors: Hong
authors: H
authors: Chen
authors: Min-xia
authors: MX
authors: Wu
authors: Mei-qin
authors: MQ
authors: Gao
authors: Shui-ping
authors: SP
authors: Chen
authors: Ai-min
authors: AM
authors: Huang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D016190:Carboplatin / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology / Q000648:ultrastructure; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000494:pharmacology; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D020869:Gene Expression Profiling; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology / Q000648:ultrastructure; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D016685:Mitomycin / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D000970:Antineoplastic Agents; D035683:MicroRNAs; D016685:Mitomycin; D004317:Doxorubicin; D016190:Carboplatin