title: Nardosinone protects H9c2 cardiac cells from angiotensin II-induced hypertrophy.
abstract: Pathological cardiac hypertrophy induced by angiotensin II (AngII) can subsequently give rise to heart failure, a leading cause of mortality. Nardosinone is a pharmacologically active compound extracted from the roots of Nardostachys chinensis, a well-known traditional Chinese medicine. In order to investigate the effects of nardosinone on AngII-induced cardiac cell hypertrophy and the related mechanisms, the myoblast cell line H9c2, derived from embryonic rat heart, was treated with nardosinone (25, 50, 100, and 200 μmol/L) or AngII (1 μmol/L). Then cell surface area and mRNA expression of classical markers of hypertrophy were detected. The related protein levels in PI3K/Akt/mTOR and MEK/ERK signaling pathways were examined by Western blotting. It was found that pretreatment with nardosinone could significantly inhibit the enlargement of cell surface area induced by AngII. The mRNA expression of ANP, BNP and β-MHC was obviously elevated in AngII-treated H9c2 cells, which could be effectively blocked by nardosinone at the concentration of 100 μmol/L. Further study revealed that the protective effects of nardosinone might be mediated by repressing the phosphorylation of related proteins in PI3K/Akt and MEK/ERK signaling pathways. It was suggested that the inhibitory effect of nardosinone on Ang II-induced hypertrophy in H9c2 cells might be mediated by targeting PI3K/Akt and MEK/ERK signaling pathways.
authors: Clinical Center for Human Genomic Research, Huazhong University of Science and Technology, Wuhan, 430022, China.|Department of Cardiovascular Diseases, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors: Meng
authors: M
authors: Du
authors: Clinical Center for Human Genomic Research, Huazhong University of Science and Technology, Wuhan, 430022, China.|Department of Cardiovascular Diseases, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors: Kun
authors: K
authors: Huang
authors: Department of Cardiovascular Diseases, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors: Lu
authors: L
authors: Gao
authors: Department of Cardiovascular Diseases, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors: Liu
authors: L
authors: Yang
authors: Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors: Wen-Shuo
authors: WS
authors: Wang
authors: Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors: Bo
authors: B
authors: Wang
authors: Clinical Center for Human Genomic Research, Huazhong University of Science and Technology, Wuhan, 430022, China. huangkai1@mail.hust.edu.cn.|Department of Cardiovascular Diseases, Huazhong University of Science and Technology, Wuhan, 430022, China. huangkai1@mail.hust.edu.cn.
authors: Kai
authors: K
authors: Huang
authors: Clinical Center for Human Genomic Research, Huazhong University of Science and Technology, Wuhan, 430022, China. joy_huangdan@yahoo.com.cn.|Department of Cardiovascular Diseases, Huazhong University of Science and Technology, Wuhan, 430022, China. joy_huangdan@yahoo.com.cn.
authors: Dan
authors: D
authors: Huang
mesh_terms: D000804:Angiotensin II / Q000502:physiology*; D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology*; D002460:Cell Line; D048429:Cell Size / Q000187:drug effects; D006984:Hypertrophy / Q000378:metabolism / Q000473:pathology; D020935:MAP Kinase Signaling System; D032386:Myoblasts, Cardiac / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism; D000081223:Polycyclic Sesquiterpenes; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012717:Sesquiterpenes / Q000494:pharmacology*; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism
chemical_list: D000804:Angiotensin II; D002316:Cardiotonic Agents; D019869:Phosphatidylinositol 3-Kinases; D000081223:Polycyclic Sesquiterpenes; D051057:Proto-Oncogene Proteins c-akt; D012333:RNA, Messenger; D012717:Sesquiterpenes; D058570:TOR Serine-Threonine Kinases; C011513:nardosinone