title: Genetic variation analysis of apple chlorotic leaf spot virus coat protein reveals a new phylogenetic type and two recombinants in China.
abstract: In this study, we generated sequences of the apple chlorotic leaf spot virus (ACLSV) coat protein (CP) gene. Genetic variation and phylogenetic analyses were carried out on these sequences along with others reported previously. ACLSV populations clustered into four types: in three of the four types, combinations of three amino acids at positions of 40, 75 and 79 were conserved. The fourth phylogenetic type, newly identified here, was characterized by co-variation of Ser(40)-Tyr(75)-Ser(79). Statistically significant genetic differentiation and infrequent gene flow were detected among the four types. Two natural recombinants were detected for the first time among ACLSV isolates/genotypes from China.
authors: State Key Laboratory of Agrobiotechnology and Department of Plant Pathology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, 100193, China.
authors: Shanyi
authors: S
authors: Chen
authors: Ying
authors: Y
authors: Zhou
authors: Ting
authors: T
authors: Ye
authors: Lu
authors: L
authors: Hao
authors: Liyun
authors: L
authors: Guo
authors: Zaifeng
authors: Z
authors: Fan
authors: Shifang
authors: S
authors: Li
authors: Tao
authors: T
authors: Zhou
mesh_terms: D036022:Capsid Proteins / Q000235:genetics*; D002681:China; D016000:Cluster Analysis; D017124:Conserved Sequence; D052677:Flexiviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral