title: Procedure for microbial identification based on Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry from screening-positive urine samples.
abstract: Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry is a widely used proteomic technique in clinical microbiology laboratories, and enables microbial identification directly from clinical samples. This study seeks to establish a protocol for bacterial identification from monomicrobial urine samples that have tested positive in the screening with Sysmex UF-1000i (Sysmex Corporation, Kobe, Japan). Sysmex UF-1000i counts ≥1 × 10(7) bacteria/mL indicate a sufficient bacterial concentration to allow direct identification from urine, with 87.5% sensitivity. Microbial identification from urine with Sysmex UF-1000i counts between 1 × 10(5) and 1 × 10(7) bacteria/ml requires preincubation to obtain the adequate amount of bacteria needed for analysis, and 91.7% sensitivity thus being achieved.
authors: Service of Microbiology, University Clinic Hospital of Valladolid, Valladolid, Spain.
authors: Gabriel A
authors: GA
authors: March Rosselló
authors: María P
authors: MP
authors: Gutiérrez Rodríguez
authors: Raúl Ortiz
authors: RO
authors: de Lejarazu Leonardo
authors: Antonio
authors: A
authors: Orduña Domingo
authors: Miguel A
authors: MA
authors: Bratos Pérez
mesh_terms: D058491:Bacterial Load; D015373:Bacterial Typing Techniques / Q000379:methods*; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000652:urine*; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis / Q000652:urine*; D006801:Humans; D018805:Sepsis / Q000175:diagnosis / Q000382:microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
keywords: MALDI-TOF; direct identification; urine