title: Preliminary crystallographic studies of BRCA1 BRCT-ABRAXAS complex.
abstract: The BRCA1 holoenzyme complex plays an important role in DNA damage repair. ABRAXAS is a newly discovered component of this complex and its C-terminal region directly binds to the BRCA1 BRCT domain. Single crystals of the BRCA1 BRCT-ABRAXAS complex grown by co-crystallization belonged to space group P4(1)2(1)2, with unit-cell parameters a = b = 187.18, c = 85.31 Å. Diffraction data were collected on the BM-14 beamline at the ESRF. Molecular-replacement calculations using Phaser led to three molecules in the asymmetric unit and a high solvent content of 76%.
authors: Advanced Centre for Treatment, Research and Education in Cancer, Kharghar, Navi Mumbai 410 210, India.
authors: Dilip C
authors: DC
authors: Badgujar
authors: Ulka
authors: U
authors: Sawant
authors: Lumbini
authors: L
authors: Yadav
authors: M V
authors: MV
authors: Hosur
authors: Ashok K
authors: AK
authors: Varma
mesh_terms: D019313:BRCA1 Protein / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: C520310:ABRAXAS1 protein, human; D019313:BRCA1 Protein; C492913:BRCA1 protein, human; D002352:Carrier Proteins; D011994:Recombinant Proteins
keywords: BRCA1 BRCT–ABRAXAS complex