title: A noncompetitive inhibitor for Mycobacterium tuberculosis's class IIa fructose 1,6-bisphosphate aldolase.
abstract: Class II fructose 1,6-bisphosphate aldolase (FBA) is an enzyme critical for bacterial, fungal, and protozoan glycolysis/gluconeogenesis. Importantly, humans lack this type of aldolase, having instead a class I FBA that is structurally and mechanistically distinct from class II FBAs. As such, class II FBA is considered a putative pharmacological target for the development of novel antibiotics against pathogenic bacteria such as Mycobacterium tuberculosis, the causative agent for tuberculosis (TB). To date, several competitive class II FBA substrate mimic-styled inhibitors have been developed; however, they lack either specificity, potency, or properties that limit their potential as possible therapeutics. Recently, through the use of enzymatic and structure-based assisted screening, we identified 8-hydroxyquinoline carboxylic acid (HCA) that has an IC50 of 10 ± 1 μM for the class II FBA present in M. tuberculosis (MtFBA). As opposed to previous inhibitors, HCA behaves in a noncompetitive manner, shows no inhibitory properties toward human and rabbit class I FBAs, and possesses anti-TB properties. Furthermore, we were able to determine the crystal structure of HCA bound to MtFBA to 2.1 Å. HCA also demonstrates inhibitory effects for other class II FBAs, including pathogenic bacteria such as methicillin-resistant Staphylococcus aureus. With its broad-spectrum potential, unique inhibitory characteristics, and flexibility of functionalization, the HCA scaffold likely represents an important advancement in the development of class II FBA inhibitors that can serve as viable preclinical candidates.
authors: Department of Chemistry and Biochemistry, University of Denver , Denver, Colorado 80208, United States.
authors: Glenn C
authors: GC
authors: Capodagli
authors: Wafik G
authors: WG
authors: Sedhom
authors: Mary
authors: M
authors: Jackson
authors: Kateri A
authors: KA
authors: Ahrendt
authors: Scott D
authors: SD
authors: Pegan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002151:Calorimetry; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D005634:Fructose-Bisphosphate Aldolase / Q000037:antagonists & inhibitors*; D006912:Hydroxyquinolines / Q000494:pharmacology*; D007700:Kinetics; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D008958:Models, Molecular; D009169:Mycobacterium tuberculosis / Q000201:enzymology*; D011817:Rabbits; D016415:Sequence Alignment
chemical_list: C587018:8-hydroxyquinolinecarboxylic acid; D006912:Hydroxyquinolines; D005634:Fructose-Bisphosphate Aldolase