title: Simultaneous kinetic spectrometric determination of three flavonoid antioxidants in fruit with the aid of chemometrics.
abstract: A simple, inexpensive and sensitive kinetic spectrophotometric method was developed for the simultaneous determination of three anti-carcinogenic flavonoids: catechin, quercetin and naringenin, in fruit samples. A yellow chelate product was produced in the presence neocuproine and Cu(I) - a reduction product of the reaction between the flavonoids with Cu(II), and this enabled the quantitative measurements with UV-vis spectrophotometry. The overlapping spectra obtained, were resolved with chemometrics calibration models, and the best performing method was the fast independent component analysis (fast-ICA/PCR (Principal component regression)); the limits of detection were 0.075, 0.057 and 0.063 mg L(-1) for catechin, quercetin and naringenin, respectively. The novel method was found to outperform significantly the common HPLC procedure.
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; Department of Chemistry, Nanchang University, Nanchang 330031, China.
authors: Ruiling
authors: R
authors: Sun
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; Department of Chemistry, Nanchang University, Nanchang 330031, China.
authors: Yong
authors: Y
authors: Wang
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; Department of Chemistry, Nanchang University, Nanchang 330031, China. Electronic address: ynni@ncu.edu.cn.
authors: Yongnian
authors: Y
authors: Ni
authors: School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology, Brisbane 4001, Australia.
authors: Serge
authors: S
authors: Kokot
mesh_terms: D000465:Algorithms; D000970:Antineoplastic Agents / Q000032:analysis; D000975:Antioxidants / Q000032:analysis*; D002392:Catechin / Q000032:analysis*; D032084:Citrus sinensis / Q000737:chemistry*; D044950:Flavanones / Q000032:analysis*; D005638:Fruit / Q000737:chemistry; D007700:Kinetics; D027845:Malus / Q000737:chemistry*; D011794:Quercetin / Q000032:analysis*; D013053:Spectrophotometry
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D044950:Flavanones; D002392:Catechin; D011794:Quercetin; C005273:naringenin
keywords: Chemometrics; Fixed-point algorithm; Flavonoids; Fruit; Kinetic spectroscopy