title: Antimicrobial activity of extracts from in vivo and in vitro propagated Lamium album L. plants.
abstract: The antimicrobial activity of 18 different extracts from in vivo and in vitro grown L. album L. plants was evaluated against clinical bacteria and yeasts using the well diffusion method. All the used extracts demonstrated antibacterial activity, whereas only the water extracts from leaves (in vivo) possessed antifungal activity against Candida albicans NBIMCC 72 and Candida glabrata NBIMCC 8673 (14 and 20 mm diameter of inhibition zones and MIC 10 mg/ml, respectively). The methanol and ethanol extracts obtained from the in vitro propagated plants had a broader spectrum of antibacterial activity than those from in vivo plants, while the opposite tendency was observed for the chloroform extracts. All tested flower extracts possessed antimicrobial activity. The chloroform extract from in vivo flowers demonstrated the highest activity against E. faecalis NBIMCC 3915, S. aureus NBIMCC 3703, P. hauseri NBIMCC 1339 and P. aeruginosa NBIMCC 3700 (22 mm, 13 mm, 11 mm, 23 mm zone diameter of inhibition and MIC 0.313 mg/ml, respectively). The water extracts from leaves (both in vivo and in vitro) possessed higher antibacterial activity than extract from flowers. The obtained results showed that both in vivo and in vitro propagated L. album L. could be used as a source of antibacterial substances.
authors: Department of General and Industrial Microbiology, Faculty of Biology, Sofia University "St. Kl. Ohridski, 8 Dragan Tsankov Blvd., 1164 Sofia, Bulgaria.
authors: Valentina Aleksandrova
authors: VA
authors: Chipeva
authors: Detelina Christova
authors: DC
authors: Petrova
authors: Milena Evgenieva
authors: ME
authors: Geneva
authors: Milena Angelova
authors: MA
authors: Dimitrova
authors: Penka Angelova
authors: PA
authors: Moncheva
authors: Veneta Michova
authors: VM
authors: Kapchina-Toteva
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects; D041221:Candida glabrata / Q000187:drug effects; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002725:Chloroform; D013293:Enterococcus faecalis / Q000187:drug effects; D000431:Ethanol; D035264:Flowers; D019686:Lamiaceae; D000432:Methanol; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D011511:Proteus / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012997:Solvents; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D010936:Plant Extracts; D012997:Solvents; D000431:Ethanol; D002725:Chloroform; D000432:Methanol
keywords: Lamium album; antimicrobial activity; in vitro propagation; plant extracts