title: Isolation of a non-urea-producing sake yeast strain carrying a discriminable molecular marker.
abstract: In the fermentation industry, the traceability of microorganisms during the process is important to ensure safety and efficacy. Ethyl carbamate, a group-2A carcinogen, is produced from ethanol and urea during the storage of food/alcoholic beverages. We isolated non-urea-producing sake yeast car1 mutants carrying a discriminable molecular marker, and demonstrated, by the use of PCR assays, that these mutants are useful for traceability analysis and identification during the sake brewing process.
authors: Niigata Prefectural Sake Research Institute.
authors: Takashi
authors: T
authors: Kuribayashi
authors: Hiroyasu
authors: H
authors: Tamura
authors: Keigo
authors: K
authors: Sato
authors: Yoshihito
authors: Y
authors: Nabekura
authors: Toshio
authors: T
authors: Aoki
authors: Yoshihiko
authors: Y
authors: Anzawa
authors: Kazuaki
authors: K
authors: Katsumata
authors: Shunji
authors: S
authors: Ohdaira
authors: Susumu
authors: S
authors: Yamashita
authors: Kazunori
authors: K
authors: Kume
authors: Mitsuoki
authors: M
authors: Kaneoke
authors: Ken-Ichi
authors: K
authors: Watanabe
authors: Dai
authors: D
authors: Hirata
mesh_terms: D000434:Alcoholic Beverages / Q000382:microbiology*; D001119:Arginase / Q000235:genetics; D015415:Biomarkers / Q000378:metabolism; D005285:Fermentation; D056426:Genetic Loci / Q000235:genetics; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D001119:Arginase