title: Functional characterization of thioredoxin 3 (TRX-3), a Caenorhabditis elegans intestine-specific thioredoxin.
abstract: Thioredoxins are a class of evolutionarily conserved proteins that have been demonstrated to play a key role in many cellular processes involving redox reactions. We report here the genetic and biochemical characterization of Caenorhabditis elegans TRX-3, the first metazoan thioredoxin with an intestine-specific expression pattern. By using green fluorescent protein reporters we have found that TRX-3 is expressed in both the cytoplasm and the nucleus of intestinal cells, with a prominent localization at the apical membrane. Although intestinal function, reproductive capacity, longevity, and resistance of trx-3 loss-of-function mutants to many stresses are indistinguishable from those of wild-type animals, we have observed a slight reduction in size and a minor reduction in the defecation cycle timing of trx-3 mutants. Interestingly, trx-3 is induced upon infection by Photorhabdus luminescens and Candida albicans, and TRX-3 overexpression provides a modest protection against these pathogens. Together, our data indicate that TRX-3 function in the intestine is dispensable for C. elegans development but may be important to fight specific bacterial and fungal infections.
authors: Centro Andaluz de Biología del Desarrollo, Departamento de Fisiología, Anatomía y Biología Celular, Universidad Pablo de Olavide, 41013 Sevilla, Spain.
authors: María
authors: M
authors: Jiménez-Hidalgo
authors: Centre d'Immunologie de Marseille-Luminy, UM2 Aix-Marseille Université, Case 906, 13288 Marseille cedex 9, France.
authors: Cyril Léopold
authors: CL
authors: Kurz
authors: Grupo de Bioquímica y Señalización Celular, Departamento de Biología Experimental, Universidad de Jaén, 23071 Jaén, Spain.
authors: José Rafael
authors: JR
authors: Pedrajas
authors: Centro Andaluz de Biología del Desarrollo, Departamento de Fisiología, Anatomía y Biología Celular, Universidad Pablo de Olavide, 41013 Sevilla, Spain.
authors: Francisco José
authors: FJ
authors: Naranjo-Galindo
authors: Centro Andaluz de Biología del Desarrollo, Departamento de Fisiología, Anatomía y Biología Celular, Universidad Pablo de Olavide, 41013 Sevilla, Spain.
authors: María
authors: M
authors: González-Barrios
authors: Center for Biomedical Research of La Rioja, 26006 Logroño, Spain.
authors: Juan
authors: J
authors: Cabello
authors: Unidad Funcional de Investigación en Enfermedades Crónicas, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain.
authors: Alberto G
authors: AG
authors: Sáez
authors: Unidad Funcional de Investigación en Enfermedades Crónicas, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain.
authors: Encarnación
authors: E
authors: Lozano
authors: Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.
authors: Emma L
authors: EL
authors: Button
authors: Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.
authors: Elizabeth A
authors: EA
authors: Veal
authors: Center for Biosciences at Novum, Department of Biosciences and Nutrition, Karolinska Institute, S-14183 Huddinge, Sweden.
authors: Juan Carlos
authors: JC
authors: Fierro-González
authors: Center for Biosciences at Novum, Department of Biosciences and Nutrition, Karolinska Institute, S-14183 Huddinge, Sweden.
authors: Peter
authors: P
authors: Swoboda
authors: Centro Andaluz de Biología del Desarrollo, Departamento de Fisiología, Anatomía y Biología Celular, Universidad Pablo de Olavide, 41013 Sevilla, Spain; Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, 41013 Sevilla, Spain. Electronic address: amiranda-ibis@us.es.
authors: Antonio
authors: A
authors: Miranda-Vizuete
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008136:Longevity / Q000235:genetics; D009928:Organ Specificity; D010084:Oxidation-Reduction; D013879:Thioredoxins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D029742:Caenorhabditis elegans Proteins; C000592487:thioredoxin 3, C elegans; D013879:Thioredoxins
keywords: Caenorhabditis elegans; Candida albicans; Intestine; Pathogen infection; Photorhabdus luminescens; Stress; Thioredoxin