title: Role of SIRT1 and AMPK in mesenchymal stem cells differentiation.
abstract: The differentiation capabilities of mesenchymal stem cells (MSCs) compromise with age and with in vitro passages which could impair the efficacy of cell therapy and tissue engineering. However, how to maintain these capabilities is not fully understood. Calorie restriction (CR, decreasing caloric intake by 30-40%) could extend longevity and reduce aging-related diseases. Recent studies revealed that CR could influence the lineage determination of stem cells including MSCs. Two important mediators of CR might be silent mating type information regulation 2 homolog 1 (SIRT1), a NAD(+)-dependent deacetylase, and AMP-activated protein kinase (AMPK), an energy-sensing kinase. Evidences are mounting that both SIRT1 and AMPK play important roles in cell fate determination of MSCs. Herein, we intend to sum up our understanding about the role of SIRT1 and AMPK in osteogenic and adipogenic potential of MSCs. Metabolic process of MSCs differentiation and the putative interplay of SIRT1 and AMPK in this process was also discussed.
authors: Department of Cardiology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, PR China; Key Lab of Cardiovascular Disease, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, PR China.
authors: Huiqiang
authors: H
authors: Chen
authors: Department of Cardiology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, PR China; Key Lab of Cardiovascular Disease, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, PR China.
authors: Xianbao
authors: X
authors: Liu
authors: Department of Cardiology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, PR China; Key Lab of Cardiovascular Disease, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, PR China.
authors: Han
authors: H
authors: Chen
authors: Key Lab of Cardiovascular Disease, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, PR China.
authors: Jiang
authors: J
authors: Cao
authors: Key Lab of Cardiovascular Disease, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, PR China.
authors: Ling
authors: L
authors: Zhang
authors: Department of Cardiology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, PR China; Key Lab of Cardiovascular Disease, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, PR China.
authors: Xinyang
authors: X
authors: Hu
authors: Department of Cardiology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, PR China; Key Lab of Cardiovascular Disease, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, PR China. Electronic address: jian_an_wang@yahoo.com.
authors: Jian'an
authors: J
authors: Wang
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D017667:Adipocytes / Q000201:enzymology; D050156:Adipogenesis; D000818:Animals; D031204:Caloric Restriction; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage; D004734:Energy Metabolism; D004789:Enzyme Activation; D020536:Enzyme Activators / Q000494:pharmacology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000201:enzymology*; D010006:Osteoblasts / Q000201:enzymology; D010012:Osteogenesis; D015398:Signal Transduction; D056564:Sirtuin 1 / Q000378:metabolism*
chemical_list: D020536:Enzyme Activators; D055372:AMP-Activated Protein Kinases; D056564:Sirtuin 1
keywords: AMPK; Calorie restriction; Mesenchymal stem cells differentiation; Resveratrol; SIRT1