title: Cell internalization SELEX: in vitro selection for molecules that internalize into cells.
abstract: Aptamer technology allows for the selection of nucleic acids that can bind to and enter cells. By establishing conditions during the selection that eliminate cell-surface binders as well as non-internalizing RNAs, only extremely tightly bound aptamers or aptamers that have internalized are recovered. We describe a general scheme for selecting RNA molecules that are capable of internalizing into cells and discuss the factors that can affect a successful selection. Much like standard cell-surface selections, these types of selections should be possible independent of detailed knowledge of the cell surface.
authors: Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY, USA.
authors: Amy
authors: A
authors: Yan
authors: Matthew
authors: M
authors: Levy
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics; D001692:Biological Transport; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008967:Molecular Biology / Q000379:methods; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D052156:SELEX Aptamer Technique; D013499:Surface Properties
chemical_list: D052157:Aptamers, Nucleotide; D012313:RNA