title: Carbon nanotube poroshell silica as a novel stationary phase for fast HPLC analysis of monoclonal antibodies.
abstract: A new carbon nanotube porous silica poroshell stationary phase was developed. The chromatographic support was coated with ultrashort single-wall carbon nanotubes (SWCNTs) in a noncovalent way. It was demonstrated that the porous amino silica surface of the 300 NH2 poroshell column stabilized with 1-methyl-2-pyrrolidinone efficiently and stably adsorbed SWCNTs onto the chromatographic support. It was shown that this novel poroshell carbon nanotube (CNT) stationary phase was very useful for the HPLC separation of a series of monoclonal antibodies (mAbs) in a short analysis time (<3 min). The high-performance liquid chromatography (HPLC) method was validated and was successfully tested for the fast quantitative and qualitative control of chemotherapeutic bags fabricated in a hospital pharmacy.
authors: Univ Franche - Comté, 25000, Besançon, France.
authors: Claire
authors: C
authors: André
authors: Rania
authors: R
authors: Aljhni
authors: Lydie
authors: L
authors: Lethier
authors: Yves Claude
authors: YC
authors: Guillaume
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis*; D002623:Chemistry Techniques, Analytical; D002851:Chromatography, High Pressure Liquid; D037742:Nanotubes, Carbon / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D037742:Nanotubes, Carbon; D012822:Silicon Dioxide