title: Assessment of 8-oxo-7,8-dihydro-2'-deoxyguanosine as a marker of oxidative DNA damage in gasoline filling station attendants.
abstract: OBJECTIVE
  The urinary excretion of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) was used as a biomarker of oxidative DNA damage. The urinary 8-oxodG levels in petrol filling station attendants (exposed) at various petrol bunks were estimated as well as in the unexposed (cashier) population.
  
  
  METHODS
  A total of 100 workers (79 petrol fillers and 21 cashiers) aged from 20 to 41 years participated in the study. An informed consent was taken from each participant. Information on personal habits and health was obtained through a questionnaire. After shifts, urine samples were collected analyzed for 8-oxodG using enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  Fifty-three percent of workers were in the 21-30 years age group. The maximum level of 8-oxodG was observed in the age group ≥ 41 years and the minimum in the age group of 31-40 years. The maximum level of 8-oxodG was observed among those workers who had ≥ 21 years of experience. The concentrations of 8-oxodG were significantly higher in petrol fillers than those in cashiers (p < 0.05).
  
  
  CONCLUSIONS
  Despite the conflicting results obtained in our study it was shown that 8-oxodG is related to chemical exposure. Further research is needed embracing a bigger number of participants to highlight the correlations between the exposure and the effects.
authors: Regional Occupational Health Center (S), Industrial Hygiene & Toxicology Division, Bangalore, India, ravichandranrohc@gmail.com.
authors: Ravichandran
authors: R
authors: Beerappa
authors: Dhananjayan
authors: D
authors: Venugopal
authors: Somnath
authors: S
authors: Sen
authors: Mala
authors: M
authors: Ambikapathy
authors: Rajmohan Hirehal Raghavendra
authors: RH
authors: Rao
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000652:urine; D004249:DNA Damage; D003839:Deoxyadenosines / Q000652:urine*; D005742:Gasoline; D006801:Humans; D019570:Inhalation Exposure; D016273:Occupational Exposure; D055815:Young Adult
chemical_list: D015415:Biomarkers; D003839:Deoxyadenosines; D005742:Gasoline; C078251:2'-deoxy-7,8-dihydro-8-oxoadenosine