title: Applications of mass spectrometry for quantitative protein analysis in formalin-fixed paraffin-embedded tissues.
abstract: Proteomic analysis of tissues has advanced in recent years as instruments and methodologies have evolved. The ability to retrieve peptides from formalin-fixed paraffin-embedded tissues followed by shotgun or targeted proteomic analysis is offering new opportunities in biomedical research. In particular, access to large collections of clinically annotated samples should enable the detailed analysis of pathologically relevant tissues in a manner previously considered unfeasible. In this paper, we review the current status of proteomic analysis of formalin-fixed paraffin-embedded tissues with a particular focus on targeted approaches and the potential for this technique to be used in clinical research and clinical diagnosis. We also discuss the limitations and perspectives of the technique, particularly with regard to application in clinical diagnosis and drug discovery.
authors: Division of Laboratory Medicine, Geneva University Hospital, Geneva, Switzerland; Human Protein Sciences Department, University of Geneva, Geneva, Switzerland; Translational Technologies and Bioinformatics, Pharma Research and Early Development, F. Hoffmann-La Roche AG, Basel, Switzerland.
authors: Carine
authors: C
authors: Steiner
authors: Axel
authors: A
authors: Ducret
authors: Jean-Christophe
authors: JC
authors: Tille
authors: Marlene
authors: M
authors: Thomas
authors: Thomas A
authors: TA
authors: McKee
authors: Laura
authors: L
authors: Rubbia-Brandt
authors: Alexander
authors: A
authors: Scherl
authors: Pierre
authors: P
authors: Lescuyer
authors: Paul
authors: P
authors: Cutler
mesh_terms: D035843:Biomedical Research / Q000379:methods; D055808:Drug Discovery; D005557:Formaldehyde; D006801:Humans; D013058:Mass Spectrometry; D016612:Paraffin Embedding; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D016707:Tissue Fixation
chemical_list: D011506:Proteins; D005557:Formaldehyde
keywords: Biomarkers; Biomedicine; Formalin-fixed paraffin-embedded tissue; Oncology; SRM; Shotgun proteomics