title: Olfactory experience modulates immature neuron development in postnatal and adult guinea pig piriform cortex.
abstract: Immature neurons expressing doublecortin (DCX+) are present around cortical layer II in various mammals including guinea pigs and humans, especially enriched in the paleocortex. However, little is known whether and how functional experience affects the development of this population of neurons. We attempted to explore a modulation by experience to layer II DCX+ cells in the primary olfactory cortex in postnatal and adult guinea pigs. Neonatal and 1-year-old guinea pigs were subjected to unilateral naris-occlusion, followed 1 and 2months later by morphometry of DCX+ cells in the piriform cortex. DCX+ somata and processes were reduced in the deprived relative to the non-deprived piriform cortex in both age groups at the two surviving time points. The number of DCX+ cells was decreased in the deprived side relative to internal control at 1 and 2months in the youths and at 2months in the adults post-occlusion. The mean somal area of DCX+ cells showed a trend of decrease in the deprived side relative to the internal control in the youths. In addition, DCX+ cells in the deprived side exhibited a lower frequency of colocalization with the neuron-specific nuclear antigen (NeuN) relative to counterparts. These results suggest that normal olfactory experience is required for the maintenance and development of DCX+ immature neurons in postnatal and adult guinea pig piriform cortex.
authors: Department of Anatomy and Neurobiology, Central South University Xiangya School of Medicine, Changsha, Hunan 410013, China.
authors: X
authors: X
authors: He
authors: Department of Neurology, The Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
authors: X-M
authors: XM
authors: Zhang
authors: Department of Neurology, The Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
authors: J
authors: J
authors: Wu
authors: Department of Neurology, The Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
authors: J
authors: J
authors: Fu
authors: Department of Anatomy and Neurobiology, Central South University Xiangya School of Medicine, Changsha, Hunan 410013, China; Department of Neurology, The Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
authors: L
authors: L
authors: Mou
authors: Department of Anatomy and Neurobiology, Central South University Xiangya School of Medicine, Changsha, Hunan 410013, China.
authors: D-H
authors: DH
authors: Lu
authors: Department of Anatomy and Neurobiology, Central South University Xiangya School of Medicine, Changsha, Hunan 410013, China.
authors: Y
authors: Y
authors: Cai
authors: Department of Anatomy and Neurobiology, Central South University Xiangya School of Medicine, Changsha, Hunan 410013, China.
authors: X-G
authors: XG
authors: Luo
authors: Department of Anatomy and Neurobiology, Central South University Xiangya School of Medicine, Changsha, Hunan 410013, China.
authors: A
authors: A
authors: Pan
authors: Department of Anatomy and Neurobiology, Central South University Xiangya School of Medicine, Changsha, Hunan 410013, China. Electronic address: yanxiaoxin@csu.edu.cn.
authors: X-X
authors: XX
authors: Yan
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000378:metabolism; D002540:Cerebral Cortex / Q000166:cytology* / Q000254:growth & development*; D003576:Electron Transport Complex IV / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006168:Guinea Pigs; D019394:Ki-67 Antigen / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D058953:Neural Stem Cells / Q000502:physiology*; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D009833:Olfactory Pathways / Q000502:physiology; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D012683:Sensory Deprivation
chemical_list: D019394:Ki-67 Antigen; D008869:Microtubule-Associated Proteins; D003576:Electron Transport Complex IV; D016282:Aspartic Acid Endopeptidases; D010751:Phosphopyruvate Hydratase; D001973:Bromodeoxyuridine
keywords: 3,3'-diaminobenzidine; ABC; ANOVA; AOI; BACE1; BrdU; CO; DAB; DCX; EPL; GL; IR; NFL; NeuN; PBS; PSA-NCAM; adult neurogenesis; analysis of variance; area of interest; avidin–biotin-complex; bromodeoxyuridine; cortical development; cytochrome c oxidase; doublecortin; external plexiform layer; functional deprivation; glomerular layer; immunoreactivity; nerve fiber layer; neuron-specific nuclear antigen; neuroplasticity; phosphate-buffered saline; polysialylated form of neural cell adhesion molecule; β-secretase-1