title: Fatty acid specificity of T1 lipase and its potential in acylglycerol synthesis.
abstract: BACKGROUND
  T1 lipase has received considerable attention due to its thermostability. Fatty acid specificity of T1 lipase (crude and purified) was investigated, and its potential in the synthesis of acylglycerols was also evaluated.
  
  
  RESULTS
  Fatty acid specificity of T1 lipase (crude and purified) was investigated in the esterification of fatty acids (C6:0 to C18:3), suggesting that crude and purified T1 lipase had the lowest preference for C18:0 [specificity constant (1/α) = 0.08] followed by C18:1 (1/α = 0.12) and showed the highest preference for C8:0 (1/α = 1). A structural model was constructed to briefly explore interactions between the lipase and its substrate. Furthermore, crude T1 lipase-catalysed synthesis of diacylglycerols (DAGs) and monoacylglycerols (MAGs) by esterification of glycerol with C18:1 was studied for evaluating its potential in acylglycerols synthesis. The optimal conditions were glycerol/oleic acid molar ratio 5:1, the lipase concentration 9.7 U g(-1) of substrates, water content 50 g kg(-1) of substrates and temperature 50 °C, which yielded 42.25% DAGs, 26.34% MAGs and 9.18% triacylglycerols at 2 h.
  
  
  CONCLUSIONS
  DAGs and MAGs were synthesised in good yields although C18:1 (a much poorer substrate) was used. Our work demonstrates that T1 lipase, which was discovered to show 1,3-regio-selectivity, is a promising biocatalyst for lipids modification.
authors: College of Food Sciences, Southwest University, Chongqing, 400715, People's Republic of China; College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, 510640, People's Republic of China.
authors: Xiao-Li
authors: XL
authors: Qin
authors: Dong-Ming
authors: DM
authors: Lan
authors: Jin-Feng
authors: JF
authors: Zhong
authors: Lu
authors: L
authors: Liu
authors: Yong-Hua
authors: YH
authors: Wang
authors: Bo
authors: B
authors: Yang
mesh_terms: D001665:Binding Sites; D002210:Caprylates / Q000378:metabolism; D004075:Diglycerides / Q000096:biosynthesis; D004795:Enzyme Stability; D004951:Esterification; D005227:Fatty Acids / Q000378:metabolism*; D056506:Geobacillus / Q000201:enzymology; D005989:Glycerides / Q000096:biosynthesis*; D006358:Hot Temperature; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D008958:Models, Molecular; D050178:Monoglycerides / Q000096:biosynthesis; D013379:Substrate Specificity
chemical_list: D002210:Caprylates; D004075:Diglycerides; D005227:Fatty Acids; D005989:Glycerides; D050178:Monoglycerides; D008049:Lipase; C031492:octanoic acid
keywords: T1 lipase; diacylglycerol; esterification; fatty acid specificity; monoacylglycerol