title: BETASEQ: a powerful novel method to control type-I error inflation in partially sequenced data for rare variant association testing.
abstract: CONCLUSIONS
  Despite its great capability to detect rare variant associations, next-generation sequencing is still prohibitively expensive when applied to large samples. In case-control studies, it is thus appealing to sequence only a subset of cases to discover variants and genotype the identified variants in controls and the remaining cases under the reasonable assumption that causal variants are usually enriched among cases. However, this approach leads to inflated type-I error if analyzed naively for rare variant association. Several methods have been proposed in recent literature to control type-I error at the cost of either excluding some sequenced cases or correcting the genotypes of discovered rare variants. All of these approaches thus suffer from certain extent of information loss and thus are underpowered. We propose a novel method (BETASEQ), which corrects inflation of type-I error by supplementing pseudo-variants while keeps the original sequence and genotype data intact. Extensive simulations and real data analysis demonstrate that, in most practical situations, BETASEQ leads to higher testing powers than existing approaches with guaranteed (controlled or conservative) type-I error.
  
  
  METHODS
  BETASEQ and associated R files, including documentation, examples, are available at http://www.unc.edu/~yunmli/betaseq
authors: Department of Biostatistics, University of North Carolina, 3101 McGavran-Greenberg Hall, Chapel Hill, NC 27599, USA, Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA and Department of Computer Science, University of North Carolina, Chapel Hill, NC 27599, USA.
authors: Song
authors: S
authors: Yan
authors: Yun
authors: Y
authors: Li
mesh_terms: D001007:Anxiety / Q000235:genetics; D016022:Case-Control Studies; D003198:Computer Simulation; D003627:Data Interpretation, Statistical; D056726:Genetic Association Studies; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D015233:Models, Statistical; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software