title: Factors other than root secreted malic acid that contributes toward Bacillus subtilis FB17 colonization on Arabidopsis roots.
abstract: The plant growth promoting rhizobacterium (PGPR) Bacillus subtilis FB17 (hereafter FB17) induces resistance against broad pathogen including Pseudomonas syringae pv tomato (PstDC3000). The extent of plant protection by FB17 depends on establishment of root colonization followed by biofilm formation. The general convention dictates that beneficial rhizobacterium may suppress the root innate immune system to establish a robust colonization. However, it is still not well understood which genetic targets FB17 affects in plants to facilitate a symbiotic association. Our recent study, involving whole transcriptome analysis of Arabdiopsis thaliana roots treated with FB17 post 24 h of treatment showed totally 279 genes that were significantly up- or/ downregulated. Further, we found that the mutants for upregulated and downregulated genes post-FB17 colonization showed a differential phenotype for FB17 root colonization. Interestingly, plants mutated in the FB17-responsive genes showed increased Aluminum activated malate transporter (ALMT1) expression under foliar pathogen PstDC3000, infections, indicating the independent functionality of ALMT1 for bacterial recruitment. Taken together this, present study suggests that the establishment of interaction between the plant host and PGPR is a complex phenomenon which is regulated by multiple genetic components.
authors: Department of Plant and Soil Sciences; University of Delaware; Newark, DE USA; Delaware Biotechnology Institute; Newark, DE USA.
authors: Venkatachalam
authors: V
authors: Lakshmanan
authors: Department of Plant and Soil Sciences; University of Delaware; Newark, DE USA; Delaware Biotechnology Institute; Newark, DE USA.
authors: Harsh P
authors: HP
authors: Bais
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001412:Bacillus subtilis / Q000254:growth & development*; D015169:Colony Count, Microbial; D015536:Down-Regulation / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D008293:Malates / Q000378:metabolism*; D027361:Organic Anion Transporters / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D059467:Transcriptome / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics
chemical_list: C512361:ALMT1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D008293:Malates; D027361:Organic Anion Transporters; C030298:malic acid
keywords: Arabidopsis; Bacillus subtilisFB17; Biofilm; Malic acid; Rhizobacteria; Root transcriptome