title: A study on anticancer activity of Caulis Spatholobi extract on human osteosarcoma Saos-2 cells.
abstract: The objective of the present study was to investigate the anticancer activity of Chinese medicine Caulis Spatholobi extract on multicentric osteosarcoma cells. Ultraviolet spectrophotometry was used to determine the total flavonoid content in each sample; vanillin sulphuric acid assay was used to determine the condensed tannin content in each sample; and the varying degrees of inhibitory activities of ethanol, ethyl acetate and n-butanol extracts of Caulis Spatholobi on human osteosarcoma Saos-2 cells were studied. The results showed that the inhibitory activity of ethyl acetate extract was the highest among the four extracts. The condensed tannin contents of 1.2 mg/mL Caulis Spatholobi water extract, ethanol extract, ethyl acetate extract and petroleum ether extract were 26.23%, 48.36%, 70.18% and 40.51% respectively; and condensed tannin content of 1.5 mg/mL Caulis Spatholobi water extract, ethanol extract, ethyl acetate extract and petroleum ether extract were 4.15%, 5.81%, 8.76% and 7.30% respectively.
authors: 3rd Orthopaedics Department, The Fourth Hospital of Jinan, Jinan, 250031, China.
authors: Bo
authors: B
authors: Liu
authors: Jinlan
authors: J
authors: Liu
authors: Jing
authors: J
authors: Chen
authors: Dongmei
authors: D
authors: Zhu
authors: Huajiang
authors: H
authors: Zhou
authors: Ximing
authors: X
authors: Wang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*; D001859:Bone Neoplasms / Q000188:drug therapy*; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D007887:Fabaceae / Q000737:chemistry*; D006801:Humans; D012516:Osteosarcoma / Q000188:drug therapy*; D008517:Phytotherapy; D018547:Plant Stems / Q000737:chemistry; D044945:Proanthocyanidins / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D044945:Proanthocyanidins
keywords: Caulis Spatholobi; Inhibitory activities; Osteosarcoma cells