title: An example of genetically distinct HIV type 1 variants in cerebrospinal fluid and plasma during suppressive therapy.
abstract: We sequenced the genome of human immunodeficiency virus type 1 (HIV-1) recovered from 70 cerebrospinal fluid (CSF) specimens and 29 plasma samples and corresponding samples obtained before treatment initiation from 17 subjects receiving suppressive therapy. More CSF sequences than plasma sequences were hypermutants. We determined CSF sequences and plasma sequences in specimens obtained from 2 subjects after treatment initiation. In one subject, we found genetically distinct CSF and plasma sequences, indicating that they came from HIV-1 from 2 different compartments, one potentially the central nervous system, during suppressive therapy. In addition, there was little evidence of viral evolution in the CSF during therapy, suggesting that continuous virus replication is not the major cause of viral persistence in the central nervous system.
authors: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet and Swedish Institute for Communicable Disease Control, Stockholm.
authors: Viktor
authors: V
authors: Dahl
authors: Magnus
authors: M
authors: Gisslen
authors: Lars
authors: L
authors: Hagberg
authors: Julia
authors: J
authors: Peterson
authors: Wei
authors: W
authors: Shao
authors: Serena
authors: S
authors: Spudich
authors: Richard W
authors: RW
authors: Price
authors: Sarah
authors: S
authors: Palmer
mesh_terms: D019380:Anti-HIV Agents / Q000627:therapeutic use*; D014644:Genetic Variation; D016679:Genome, Viral; D015658:HIV Infections / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000188:drug therapy / Q000821:virology; D015497:HIV-1 / Q000145:classification / Q000235:genetics*; D006801:Humans; D008297:Male; D012367:RNA, Viral / Q000235:genetics; D019562:Viral Load
chemical_list: D019380:Anti-HIV Agents; D012367:RNA, Viral
keywords: CNS; CSF; HIV; central nervous system; cerebrospinal fluid; compartmentalization; hypermutants; reservoir; suppressive; therapy