title: Photobleaching imprinting microscopy: seeing clearer and deeper.
abstract: We present a generic sub-diffraction-limited imaging method - photobleaching imprinting microscopy (PIM) - for biological fluorescence imaging. A lateral resolution of 110 nm was measured, more than a twofold improvement over the optical diffraction limit. Unlike other super-resolution imaging techniques, PIM does not require complicated illumination modules or specific fluorescent dyes. PIM is expected to facilitate the conversion of super-resolution imaging into a routine lab tool, making it accessible to a much broader biological research community. Moreover, we show that PIM can increase the image contrast of biological tissue, effectively extending the fundamental depth limit of multi-photon fluorescence microscopy.
authors: Department of Biomedical Engineering, Washington University in St Louis, One Brookings Dr., St Louis, MO 63130, USA.
authors: Liang
authors: L
authors: Gao
authors: Alejandro
authors: A
authors: Garcia-Uribe
authors: Yan
authors: Y
authors: Liu
authors: Chiye
authors: C
authors: Li
authors: Lihong V
authors: LV
authors: Wang
mesh_terms: D000818:Animals; D003116:Color; D005456:Fluorescent Dyes / Q000378:metabolism; D021621:Imaging, Three-Dimensional; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton; D038761:Photobleaching
chemical_list: D005456:Fluorescent Dyes
keywords: Fluorescence; Microscopy; Photobleaching; Super-resolution