title: The effect of different temperatures on fatty-acid synthesis and polyunsaturation in cell suspension cultures.
abstract: Cell suspension cultures of Catharanthus roseus G. Don, Glycine max (L.) Merr. and Nicotiana tabacum L. were incubated with [(14)C]acetate, [(14)C]oleic acid and [(14)C]linoleic acid at five different temperatures ranging from 15 to 35° C. When the incubation temperature was increased, [(14)C]acetate was incorporated preferentially into [(14)C]palmitate, with a concomitant drop in [(14)C]oleate formation. Between 15 and 20° C, [(14)C]oleic acid accumulated in C. roseus cells. In all cultures, optimum desaturation of [(14)C]oleic acid to [(14)C]linoleic acid occurred between 20 and 25° C, and in G. max this was also the optimal range for desaturation of [(14)C]linoleic acid to [(14)C]linolenic acid. Elongation of [(14)C]palmitic acid was inhibited when cultures grown at 15° C for 25 h were subsequently incubated with [(14)C]acetate at 25° C. [(14)C]oleic acid accumulated in G. max and C. roseus cultures grown at 35° C for 25 h and subsequently incubated at 25° C. Desaturation of [(14)C]oleic acid increased up to 25° C, but then decreased or leveled off depending on the cell line and on the temperature prior to incubation.
authors: Department of Biochemistry and Biophysics, University of California, 95616, Davis, CA, USA.
authors: J J
authors: JJ
authors: Maccarthy
authors: P K
authors: PK
authors: Stumpf