title: Gypenosides pre-treatment protects the brain against cerebral ischemia and increases neural stem cells/progenitors in the subventricular zone.
abstract: Gypenosides (GPs) have been reported to have neuroprotective effects in addition to other bioactivities. The protective activity of GPs during stroke and their effects on neural stem cells (NSCs) in the ischemic brain have not been fully elucidated. Here, we test the effects of GPs during stroke and on the NSCs within the subventricular zone (SVZ) of middle cerebral artery occlusion (MCAO) rats. Our results show that pre-treatment with GPs can reduce infarct volume and improve motor function following MCAO. Pre-treatment with GPs significantly increased the number of BrdU-positive cells in the ipsilateral and contralateral SVZ of MCAO rats. The proliferating cells in both sides of the SVZ were glial fibrillary acidic protein (GFAP)/nestin-positive type B cells and doublecortin (DCX)/nestin-positive type A cells. Our data indicate that GPs have neuroprotective effects during stroke which might be mediated through the enhancement of neurogenesis within the SVZ. These findings provide new evidence for a potential therapy involving GPs for the treatment of stroke.
authors: Department of Cell Biology, School of Medicine, Shandong University, Jinan, Shandong, People's Republic of China; Department of Neurobiology, Shandong Provincial Key Laboratory of Mental Disorders, School of Medicine, Shandong University, Jinan, Shandong, People's Republic of China.
authors: Xiao-Jing
authors: XJ
authors: Wang
authors: Department of Neurobiology, Shandong Provincial Key Laboratory of Mental Disorders, School of Medicine, Shandong University, Jinan, Shandong, People's Republic of China.
authors: Tao
authors: T
authors: Sun
authors: Department of Neurobiology, Shandong Provincial Key Laboratory of Mental Disorders, School of Medicine, Shandong University, Jinan, Shandong, People's Republic of China.
authors: Liang
authors: L
authors: Kong
authors: Department of Cell Biology, School of Medicine, Shandong University, Jinan, Shandong, People's Republic of China.
authors: Zhen-Hua
authors: ZH
authors: Shang
authors: Department of Cell Biology, School of Medicine, Shandong University, Jinan, Shandong, People's Republic of China.
authors: Kun-Qi
authors: KQ
authors: Yang
authors: Department of Cell Biology, School of Medicine, Shandong University, Jinan, Shandong, People's Republic of China.
authors: Qing-Yu
authors: QY
authors: Zhang
authors: Department of Cell Biology, School of Medicine, Shandong University, Jinan, Shandong, People's Republic of China.
authors: Fang-Miao
authors: FM
authors: Jing
authors: Department of Cell Biology, School of Medicine, Shandong University, Jinan, Shandong, People's Republic of China.
authors: Lun
authors: L
authors: Dong
authors: Department of Neurobiology, Shandong Provincial Key Laboratory of Mental Disorders, School of Medicine, Shandong University, Jinan, Shandong, People's Republic of China.
authors: Xu-Feng
authors: XF
authors: Xu
authors: Department of Cell Biology, School of Medicine, Shandong University, Jinan, Shandong, People's Republic of China.
authors: Jia-Xin
authors: JX
authors: Liu
authors: Department of Cell Biology, School of Medicine, Shandong University, Jinan, Shandong, People's Republic of China.
authors: Hua
authors: H
authors: Xin
authors: Department of Neurobiology, Shandong Provincial Key Laboratory of Mental Disorders, School of Medicine, Shandong University, Jinan, Shandong, People's Republic of China. Electronic address: zheyuchen@sdu.edu.cn.
authors: Zhe-Yu
authors: ZY
authors: Chen
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D020520:Brain Infarction / Q000209:etiology / Q000517:prevention & control; D002545:Brain Ischemia / Q000150:complications / Q000473:pathology* / Q000517:prevention & control*; D001973:Bromodeoxyuridine; D002552:Cerebral Ventricles / Q000187:drug effects*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D000089662:Doublecortin Protein; D029774:Gynostemma; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009422:Nervous System Diseases / Q000209:etiology / Q000517:prevention & control; D058953:Neural Stem Cells / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D010936:Plant Extracts / Q000627:therapeutic use; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D009419:Nerve Tissue Proteins; D018696:Neuroprotective Agents; D010936:Plant Extracts; C474918:gypenoside; D001973:Bromodeoxyuridine
keywords: Cell proliferation; Gypenosides; Neurogenesis; Stroke; Subventricular zone