title: Immunohistochemial study on the expression of von Willebrand factor (vWF) after onlay autogenous iliac grafts for lateral alveolar ridge augmentation.
abstract: BACKGROUND
  The main problems of autogenous bone transplants are their unpredictable atrophy and their loss of structure. One key factor lies in the poor revascularization of simple onlay grafts. The the aim of this study was to evaluate the revascularization processes in autogenous bone grafts from the iliac crest to the alveolar ridge.
  
  
  METHODS
  In a sheep model, autogenous bone grafts were harvested from the iliac crest. A combination of a resorbable collagen membrane (CM) and deproteinized bovine bone material (DBBM) was used to modify the bone graft (experiment 2). This was compared with a simple onlay bone graft (control group, experiment 1). The amount of vessels in bone and connective tissue (CT), and the amount of CT were analyzed. The expression of von Willebrand factor (vWF) was compared between the two experimental groups using immunohistochemical analysis.
  
  
  RESULTS
  The ratio of the amount of vessels in bone and CT changed over time, and more vessels could be detected in bone at 12-16 weeks of graft healing. The number of vessels were significantly higher in experiment 2 than in experiment 1. More CT was found in experiment 1, whereas the amount of CT in both experiments decreased over time.
  
  
  CONCLUSIONS
  This study shows a more intensive and extensive revascularization in experiment 2, as significantly more vessels were detected. The decreased amount of CT in experiment 2 clarifies its clinical superiority.
authors: Department of Oral and Maxillofacial Plastic Surgery, University of Wuerzburg, Pleicherwall 2, Wuerzburg D-97070, Germany. steffen.koerdt@web.de.
authors: Steffen
authors: S
authors: Koerdt
authors: Joerg
authors: J
authors: Siebers
authors: Wilhelm
authors: W
authors: Bloch
authors: Oliver
authors: O
authors: Ristow
authors: Alexander C
authors: AC
authors: Kuebler
authors: Tobias
authors: T
authors: Reuther
mesh_terms: D000540:Alveolar Ridge Augmentation / Q000379:methods*; D000818:Animals; D064592:Autografts; D003094:Collagen; D003238:Connective Tissue / Q000098:blood supply / Q000378:metabolism; D005260:Female; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D007085:Ilium / Q000637:transplantation*; D007150:Immunohistochemistry; D007284:Inlays; D008567:Membranes, Artificial; D018919:Neovascularization, Physiologic / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology; D012756:Sheep; D014945:Wound Healing / Q000502:physiology; D014841:von Willebrand Factor / Q000378:metabolism*
chemical_list: D008567:Membranes, Artificial; D014841:von Willebrand Factor; D003094:Collagen