title: Analysis of 20alpha-hydroxysteroid dehydrogenase expression in the corpus luteum of the buffalo cow: effect of prostaglandin F2-alpha treatment on circulating 20alpha-hydroxyprogesterone levels.
abstract: BACKGROUND
  During female reproductive cycles, a rapid fall in circulating progesterone (P4) levels is one of the earliest events that occur during induced luteolysis in mammals. In rodents, it is well recognized that during luteolysis, P4 is catabolized to its inactive metabolite, 20alpha-hydroxyprogesterone (20alpha-OHP) by the action of 20alpha-hydroxysteroid dehydrogenase (20alpha-HSD) enzyme and involves transcription factor, Nur77. Studies have been carried out to examine expression of 20alpha-HSD and its activity in the corpus luteum (CL) of buffalo cow.
  
  
  METHODS
  The expression of 20alpha-HSD across different bovine tissues along with CL was examined by qPCR analysis. Circulating P4 levels were monitored before and during PGF2alpha treatment. Expression of 20alpha-HSD and Nur77 mRNA was determined in CL at different time points post PGF2alpha treatment in buffalo cows. The chromatographic separation of P4 and its metabolite, 20alpha-OHP, in rat and buffalo cow serum samples were performed on reverse phase HPLC system. To further support the findings, 20alpha-HSD enzyme activity was quantitated in cytosolic fraction of CL of both rat and buffalo cow.
  
  
  RESULTS
  Circulating P4 concentration declined rapidly in response to PGF2alpha treatment. HPLC analysis of serum samples did not reveal changes in circulating 20alpha-OHP levels in buffalo cows but serum from pseudo pregnant rats receiving PGF2alpha treatment showed an increased 20alpha-OHP level at 24 h post treatment with accompanying decrease in P4 concentration. qPCR expression of 20alpha-HSD in CL from control and PGF2alpha-treated buffalo cows showed higher expression at 3 and 18 h post treatment, but its specific activity was not altered at different time points post PGF2alpha treatment. The Nur77 expression increased several fold 3 h post PGF2alpha treatment similar to the increased expression observed in the PGF2alpha-treated pseudo pregnant rats which perhaps suggest initiation of activation of apoptotic pathways in response to PGF2alpha treatment.
  
  
  CONCLUSIONS
  The results taken together suggest that synthesis of P4 appears to be primarily affected by PGF2alpha treatment in buffalo cows in contrast to increased metabolism of P4 in rodents.
authors: Tripathy
authors: T
authors: Sudeshna
authors: Kumarasamy
authors: K
authors: Anand
authors: Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560012, India. rmm@mrdg.iisc.ernet.in.
authors: Rudraiah
authors: R
authors: Medhamurthy
mesh_terms: D015089:20-Hydroxysteroid Dehydrogenases / Q000097:blood / Q000378:metabolism*; D000818:Animals; D002020:Buffaloes / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003338:Corpus Luteum / Q000201:enzymology*; D015237:Dinoprost / Q000494:pharmacology*; D005260:Female; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011374:Progesterone / Q000097:blood*; D051381:Rats
chemical_list: D011374:Progesterone; D015237:Dinoprost; D015089:20-Hydroxysteroid Dehydrogenases