title: Monoclonal antibodies against NS4B protein of japanese encephalitis virus.
abstract: Japanese encephalitis (JE) is one of the most prevalent global viral encephalitis viruses. The functions of JEV (virus) NS4B protein are still under investigation. In our study, NS4B was expressed in Escherichia coli and purified by dialysis. Two clones of monoclonal antibodies (MAbs 1B1 and 1C3) against NS4B protein were generated and their characterizations were investigated. IFA, Western blot, and ELISA results showed that the MAbs were specific against JEV NS4B protein. The epitope of the MAbs was further identified using pairs of synthesized overlapping peptides. These MAbs may provide valuable tools for further exploration of the functions of NS4B and the pathogenesis of Japanese encephalitis virus.
authors: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University , Wuhan, Hubei, China .
authors: Xindi
authors: X
authors: Ruan
authors: Shaomei
authors: S
authors: Huang
authors: Lin
authors: L
authors: Shao
authors: Jing
authors: J
authors: Ye
authors: Zheng
authors: Z
authors: Chen
authors: Huanchun
authors: H
authors: Chen
authors: Shengbo
authors: S
authors: Cao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000096:biosynthesis / Q000737:chemistry* / Q000276:immunology; D000918:Antibody Specificity; D015153:Blotting, Western; D002460:Cell Line; D006224:Cricetinae; D004664:Encephalitis Virus, Japanese / Q000276:immunology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006825:Hybridomas; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000737:chemistry* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D007074:Immunoglobulin G; C080107:NS4B protein, flavivirus; D017361:Viral Nonstructural Proteins