title: Detection and isolation of 2009 pandemic influenza A/H1N1 virus in commercial piggery, Lagos Nigeria.
abstract: WHO declared pandemic of A/H1N1 influenza in 2009 following global spread of the newly emerged strain of the virus from swine. Presently there is a dearth of data on the ecology of pandemic influenza H1N1 required for planning of intervention measures in sub Saharan Africa. Herein we report isolation of 2009 pandemic influenza A/H1N1 in an intensive mega piggery farms operation in South West Nigeria. Sentinel surveillance was carried out in a cohort of intensively reared pigs over a period of two years. Nasal swab specimens were collected at monthly interval from observed clinical cases of influenza like illness in pigs and pig handlers. Samples were analyzed by real time RT-PCR and isolation in chicken embryonated eggs. A total of 227 clinical cases of influenza like illness were observed among pigs out of which 31 (13.7%) were positive for influenza A matrix gene by real time RT-PCR. Virus isolation yielded 29 (12%) isolates out of which 18 (18%) were identified as influenza A/H1N1 by Heamaglutination Inhibition test using H1 antisera. RT-PCR positive samples were subtyped as 2009 pandemic A/H1N1 with subtype specific primers and probes. This is the first report of detection and isolation of pandemic influenza H1N1 from pigs in Nigeria. Continuous circulation of this virus in pigs may cause reassortments with seasonal influenza or mutations and substitutions in the gene that may result in the emergence of novel or pandemic influenza virus of economic and public health importance. Nigeria is considered a geographical hotspot of zoonotic diseases, which necessitate active surveillance and monitoring of emerging pandemic threats.
authors: Virology Department, College of Medicine, University of Ibadan, Ibadan, Nigeria; Regional Center for Animal Influenza, National Veterinary Research Institute, Vom, Nigeria. Electronic address: cameseko@yahoo.com.
authors: C A
authors: CA
authors: Meseko
authors: Regional Center for Animal Influenza, National Veterinary Research Institute, Vom, Nigeria. Electronic address: foreodaibo@hotmail.com.
authors: G N
authors: GN
authors: Odaibo
authors: Regional Center for Animal Influenza, National Veterinary Research Institute, Vom, Nigeria. Electronic address: ibvirology@yahoo.com.
authors: D O
authors: DO
authors: Olaleye
mesh_terms: D000818:Animals; D005260:Female; D006389:Hemagglutinins, Viral / Q000235:genetics; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D008297:Male; D009549:Nigeria; D009976:Orthomyxoviridae Infections / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000473:pathology / Q000821:virology*
chemical_list: D006389:Hemagglutinins, Viral
keywords: Detection; Isolation; Nigeria; Pandemic influenza; Pigs