title: miR-24 and miR-205 expression is dependent on HPV onco-protein expression in keratinocytes.
abstract: A screen of microRNA (miRNA) expression following differentiation in human foreskin keratinocytes (HFKs) identified changes in several miRNAs, including miR-24 and miR-205. We investigated how expression of Human Papilloma Virus Type-16 (HPV16) onco-proteins E6 and E7 affected expression of miR-24 and miR-205 during proliferation and differentiation of HFKs. We show that the induction of both miR-24 and miR-205 observed during differentiation of HFKs is lost in HFKs expressing E6 and E7. We demonstrate that the effect on miR-205 is due to E7 activity, as miR-205 expression is dependent on pRb expression. Finally, we provide evidence that miR-24 effects in the cell may be due to targeting of cyclin dependent kinase inhibitor p27. In summary, these results indicate that expression of both miR-24 and miR-205 are impacted by E6 and/or E7 expression, which may be one mechanism by which HPV onco-proteins can disrupt the balance between proliferation and differentiation in keratinocytes.
authors: Biomedical Sciences Research Institute, University of Ulster, Coleraine, Co. Derry BT52 1SA, UK; Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen's University Belfast, Belfast BT9 7BL, UK. Electronic address: dj.mckenna@ulster.ac.uk.
authors: Declan J
authors: DJ
authors: McKenna
authors: Daksha
authors: D
authors: Patel
authors: Dennis J
authors: DJ
authors: McCance
mesh_terms: D049109:Cell Proliferation; D052816:Foreskin / Q000166:cytology / Q000378:metabolism / Q000821:virology; D052162:Human papillomavirus 16 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism* / Q000821:virology; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D050725:Papillomavirus E7 Proteins / Q000235:genetics / Q000378:metabolism*; D030361:Papillomavirus Infections / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology / Q000821:virology; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C068651:E6 protein, Human papillomavirus type 16; C534425:MIR205, human; C543845:MIRN24 microRNA, human; C059731:oncogene protein E7, Human papillomavirus type 16
keywords: Differentiation; HPV; Keratinocytes; miR-205; miR-24; microRNA