title: New triterpene saponins from the roots of Glycyrrhiza yunnanensis and their rapid screening by LC/MS/MS.
abstract: The roots of Glycyrrhiza yunnanensis Cheng f. et L. K. Dai ex P. C. Li are used as licorice in traditional medicine of Southwest China. Triterpene saponins are the major chemical constituents. In this study, one new oleanane-type triterpenoid, glyyunnansapogenin I (I), seven new triterpene saponins, yunganosides E3 (II), L (III), M (IV), N1 (V), O (VI), P (VII) and N2 (VIII), together with four known saponins (IX-XII) were isolated from the roots of G. yunnanensis by preparative chromatography. Their structures were identified by spectroscopic analysis including NMR and HR-MS. Based on (-)-ESI-MS/MS fragmentation behaviors of these reference standards, an LC/MS/MS method using neutral loss scan and precursor ion scan on a triple quadrupole mass spectrometer was established to rapidly and comprehensively analyze triterpene saponins in G. yunnanensis. Combined with high-accuracy qTOF mass spectrometry analysis, a total of 50 saponins were detected, and their structures were identified or tentatively characterized. This is the first systematic study on triterpene saponins in G. yunnanensis.
authors: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors: Shuai
authors: S
authors: Ji
authors: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors: Qing
authors: Q
authors: Wang
authors: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors: Xue
authors: X
authors: Qiao
authors: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors: Hong-cheng
authors: HC
authors: Guo
authors: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors: Yan-fang
authors: YF
authors: Yang
authors: Agilent Technologies, 3 Wangjing North Road, Beijing 100102, China.
authors: Tao
authors: T
authors: Bo
authors: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors: Cheng
authors: C
authors: Xiang
authors: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors: De-an
authors: DA
authors: Guo
authors: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. Electronic address: yemin@bjmu.edu.cn.
authors: Min
authors: M
authors: Ye
mesh_terms: D002681:China; D002853:Chromatography, Liquid / Q000379:methods*; D006035:Glycyrrhiza / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008516:Medicine, Chinese Traditional; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D018517:Plant Roots; D012503:Saponins / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014315:Triterpenes / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes
keywords: Glycyrrhiza yunnanensis; Glyyunnansapogenin I; LC/MS/MS; Triterpene saponin; Yunganoside