title: Is the signal from the mesophyll to the guard cells a vapour-phase ion?
abstract: Previous studies have suggested that the red light and CO2 responses of stomata are caused by a signal from the mesophyll to the guard cells. Experiments were conducted to test the idea that this signal is a vapour-phase ion. Stomata in isolated epidermes of Tradescantia pallida were found to respond to air ions created by an electrode that was positioned under the epidermes. Anthocyanins in the epidermes of this species were observed to change colour in response to these air ions, and this change in colour was attributed to changes in pH. A similar change in lower epidermal colour was observed in intact leaves upon illumination and with changes in CO2 concentration. Based on the change in epidermal colour, the pH of the epidermis was estimated to be approximately 7.0 in darkness and 6.5 in the light. Stomata in isolated epidermes responded to pH when suspended over (but not in contact with) solutions of different pH. We speculate that stomatal responses to CO2 and light are caused by vapour-phase ions, possibly hydronium ions that change the pH of the epidermis.
authors: Biology Department, Utah State University, Logan, UT, 84322-5305, USA.
authors: Keith A
authors: KA
authors: Mott
authors: David G
authors: DG
authors: Berg
authors: Sean M
authors: SM
authors: Hunt
authors: David
authors: D
authors: Peak
mesh_terms: D000872:Anthocyanins / Q000378:metabolism; D004566:Electrodes; D007477:Ions; D058503:Mesophyll Cells / Q000166:cytology / Q000378:metabolism*; D044367:Phase Transition; D054046:Plant Stomata / Q000166:cytology* / Q000378:metabolism; D012996:Solutions; D034823:Tradescantia / Q000378:metabolism* / Q000502:physiology; D014835:Volatilization
chemical_list: D000872:Anthocyanins; D007477:Ions; D012996:Solutions
keywords: CO2; epidermis; guard cells; light; pH; signal; stomata