title: Functional analysis of BpDREB2 gene involved in salt and drought response from a woody plant Broussonetia papyrifera.
abstract: The dehydration-responsive element binding proteins (DREBs) are important transcription factors in the regulation of plant responses to abiotic stresses. In this study, BpDREB2, an AP2/DREB-type transcription factor gene, was cloned from a woody plant, Broussonetia papyrifera by RACE-PCR. Sequence analyses revealed that BpDREB2 protein has three characteristic domains, including an AP2/EREBP, a nuclear localization signal and an acidic activation domain. Yeast one-hybrid assays showed that BpDREB2 protein specifically binds to the DRE sequence and activates the expression of reporter genes in yeast. These results suggested that BpDREB2 protein could function as a transcription factor of DREB family. The expression of BpDREB2 gene was remarkably induced by dehydration and high-salt treatments, but no significant change was observed under ABA or low-temperature conditions. Importantly, transgenic expression of BpDREB2 gene in Arabidopsis significantly enhanced its tolerance to salt and freezing without causing growth retardation. Taken together, these results suggested that BpDREB2 is a novel member of the AP2/EREBP trans-acting factor family which could enhance salt stress tolerance of plants and has the potential application in the improvement of crops and economical tree species.
authors: Key Laboratory of Plant Resources, Institute of Botany, the Chinese Academy of Sciences, Beijing, PR China; Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing,100081, PR China.
authors: Jingwen
authors: J
authors: Sun
authors: Key Laboratory of Plant Resources, Institute of Botany, the Chinese Academy of Sciences, Beijing, PR China.
authors: Xianjun
authors: X
authors: Peng
authors: Key Laboratory of Plant Resources, Institute of Botany, the Chinese Academy of Sciences, Beijing, PR China.
authors: Weihong
authors: W
authors: Fan
authors: Key Laboratory of Plant Resources, Institute of Botany, the Chinese Academy of Sciences, Beijing, PR China.
authors: Mingjuan
authors: M
authors: Tang
authors: Key Laboratory of Plant Resources, Institute of Botany, the Chinese Academy of Sciences, Beijing, PR China.
authors: Jie
authors: J
authors: Liu
authors: Key Laboratory of Plant Resources, Institute of Botany, the Chinese Academy of Sciences, Beijing, PR China. Electronic address: shshen@ibcas.ac.cn.
authors: Shihua
authors: S
authors: Shen
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D031623:Broussonetia / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D003080:Cold Temperature; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D023061:Gene Order; D005822:Genetic Vectors / Q000235:genetics; D042541:Intracellular Space / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D010641:Phenotype; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011485:Protein Binding; D021381:Protein Transport; D020218:Response Elements; D055049:Salt Tolerance / Q000235:genetics*; D016415:Sequence Alignment; D013312:Stress, Physiological / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D015533:Transcriptional Activation; D020798:Two-Hybrid System Techniques
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D014157:Transcription Factors
keywords: ABA; ABA-responsive element; ABRE; AP2/EREBP-domain; AP2/ERF; APETALA 2/ERE binding factor; Broussonetia papyrifera; C-repeat binding factor; CBF; CaMV; DRE; DRE-binding protein; DRE/CRT; DREB; GFP; RACE; Salt and cold tolerance; Woody plant; abscisic acid; cauliflower mosaic virus; dehydration-responsive element; dehydration-responsive element/C-repeat; green fluorescent protein; mDRE; mutant dehydration-responsive element; rapid amplification of cDNA ends