title: Synthesis and use of QCy7-derived modular probes for the detection and imaging of biologically relevant analytes.
abstract: This protocol describes the synthesis of modular turn-ON QCy7-based probes for the detection of biologically relevant analytes, such as hydrogen peroxide, ubiquitous sulfhydryl and β-galactosidase. The probes presented herein are prepared through a simple procedure that involves the preliminary alkylation of 4-hydroxy-isophthalaldehyde with a relevant analyte-responsive protecting group, followed by condensation of the resulting product with 2 equivalents of sulfo-indolium moieties. Evaluation of the turn-ON near-IR fluorescence response to their relevant analytes for the three different QCy7 probes is also reported. The preparation of a QCy7 diagnostic probe requires 1-2 d. Probes for other analytes can be prepared according to this modular procedure by incorporating a specific analyte-responsive group as a triggering substrate.
authors: School of Chemistry, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, Israel.
authors: Orit
authors: O
authors: Redy-Keisar
authors: Einat
authors: E
authors: Kisin-Finfer
authors: Shiran
authors: S
authors: Ferber
authors: Ronit
authors: R
authors: Satchi-Fainaro
authors: Doron
authors: D
authors: Shabat
mesh_terms: D052160:Benzothiazoles / Q000032:analysis / Q000737:chemistry*; D002232:Carbocyanines / Q000032:analysis / Q000737:chemistry*; D060326:Chemistry Techniques, Synthetic; D005456:Fluorescent Dyes / Q000032:analysis / Q000138:chemical synthesis*; D011809:Quinones / Q000032:analysis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D052160:Benzothiazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D011809:Quinones; C011204:cyanine dye 7