title: Differential pharmacokinetics and the brain distribution of morphine and ephedrine constitutional isomers in rats after oral administration with Keke capsule using rapid-resolution LC-MS/MS.
abstract: Opioid and ephedra alkaloids known as the active ingredients for Keke capsule, which is used to treat coughs and bronchial asthma, could have potential adverse effects on the central nervous system. Therefore, an efficient, sensitive rapid-resolution LC-MS/MS method for the simultaneous determination of morphine, ephedrine, and pseudoephedrine in rat plasma and brain tissue homogenate has been developed. The method was validated in the plasma and brain tissue samples, showed good linearity over a wide concentration range (r(2) > 0.99). The intra- and interday assay variability was less than 15% for all analytes, and the accuracy was between -8.8 and 5.7%. The study provided the pharmacokinetics profiles and the brain regional distribution of the three active alkaloids after oral administration of Keke capsule. The results also indicated that significant difference in pharmacokinetics parameters of the epimers was observed between ephedrine and pseudoephedrine.
authors: The National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang, China; College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, China.
authors: Yonggui
authors: Y
authors: Song
authors: Dan
authors: D
authors: Su
authors: Tulin
authors: T
authors: Lu
authors: Chunqin
authors: C
authors: Mao
authors: De
authors: D
authors: Ji
authors: Yali
authors: Y
authors: Liu
authors: Binbin
authors: B
authors: Wei
authors: Ronghua
authors: R
authors: Fan
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001921:Brain; D002851:Chromatography, High Pressure Liquid; D004809:Ephedrine / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D008297:Male; D008968:Molecular Conformation; D009020:Morphine / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry; D014018:Tissue Distribution
chemical_list: D009020:Morphine; D004809:Ephedrine
keywords: Ephedrine; Keke capsule; Morphine; Pharmacokinetics; Pseudoephedrine