title: Induction of meiotic gynogenesis in ship sturgeon Acipenser nudiventris using UV-irradiated heterologous sperm.
abstract: Diploid gynogenesis was induced in ship sturgeon Acipenser nudiventris using UV-irradiated sperm from Siberian sturgeon Acipenser baerii. The optimal condition for the retention of the second polar body in ship sturgeon was determined to be 10 min after activation/fertilization in experiments. The temperature of cold shock and its duration were 2.5 °C and 30 min, respectively. A total of 30 gynogens of known parentage from experimental treatments were screened using microsatellite DNA analysis, and uniparental transmission in meiogens was confirmed. The results show that heterologous Siberian sturgeon sperm is applicable as UV-irradiated sperm for the induction of gynogenesis in ship sturgeon. This technique may recover the critically endangered sturgeon species that are becoming extinct.
authors: Genetics Department, International Sturgeon Research Institute, P.O. Box 41635-3464, Rasht, Iran, saber_meraj@yahoo.com.
authors: Mohammad
authors: M
authors: Hassanzadeh Saber
authors: Shahrouz Baradaran
authors: SB
authors: Noveiri
authors: Mohammad
authors: M
authors: Pourkazemi
authors: Mohammadali
authors: M
authors: Yazdani
authors: Ahmad
authors: A
authors: Ghoroghi
authors: Mahmoud
authors: M
authors: Bahmani
authors: Mohammad
authors: M
authors: Pourdehghani
authors: Fereidoon
authors: F
authors: Chakmehdouz
authors: Mahtab
authors: M
authors: Yarmohammadi
authors: Mohammadreza
authors: M
authors: Nowruzfashkhami
mesh_terms: D000483:Alleles; D000818:Animals; D005260:Female; D005399:Fishes / Q000033:anatomy & histology / Q000235:genetics*; D016678:Genome / Q000235:genetics; D040582:Inheritance Patterns / Q000235:genetics* / Q000528:radiation effects; D007814:Larva / Q000166:cytology / Q000235:genetics / Q000528:radiation effects; D008297:Male; D008540:Meiosis / Q000235:genetics / Q000528:radiation effects*; D012767:Ships; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*; D016019:Survival Analysis; D014466:Ultraviolet Rays