title: The role of energy dissipation of polymeric scaffolds in the mechanobiological modulation of chondrogenic expression.
abstract: Mechanical stimulation has been proposed to induce chondrogenesis in cell-seeded scaffolds. However, the effects of mechanical stimuli on engineered cartilage may vary substantially between different scaffolds. This advocates for the need to identify an overarching mechanobiological variable. We hypothesize that energy dissipation of scaffolds subjected to dynamic loading may be used as a mechanobiology variable. The energy dissipation would furnish a general criterion to adjust the mechanical stimulation favoring chondrogenesis in scaffold. Epiphyseal chondro-progenitor cells were then subject to unconfined compression 2 h per day during four days in different scaffolds, which differ only by the level of dissipation they generated while keeping the same loading conditions. Scaffolds with higher dissipation levels upregulated the mRNA of chondrogenic markers. In contrast lower dissipation of scaffolds was associated with downregulation of chondrogenic markers. These results showed that energy dissipation could be considered as a mechanobiology variable in cartilage. This study also indicated that scaffolds with energy dissipation level close to the one of cartilage favors chondrogenic expression when dynamical loading is present.
authors: Laboratory of Biomechanical Orthopedics, Institute of Bioengineering, EPF Lausanne, Switzerland.
authors: Philippe
authors: P
authors: Abdel-Sayed
authors: Laboratory of Biomechanical Orthopedics, Institute of Bioengineering, EPF Lausanne, Switzerland.
authors: Salim E
authors: SE
authors: Darwiche
authors: Laboratory of Biomechanical Orthopedics, Institute of Bioengineering, EPF Lausanne, Switzerland.
authors: Ulrike
authors: U
authors: Kettenberger
authors: Laboratory of Biomechanical Orthopedics, Institute of Bioengineering, EPF Lausanne, Switzerland. Electronic address: dominique.pioletti@epfl.ch.
authors: Dominique P
authors: DP
authors: Pioletti
mesh_terms: D002356:Cartilage / Q000737:chemistry; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000502:physiology; D006801:Humans; D011108:Polymers / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011108:Polymers
keywords: Cartilage; Chondrogenic expression; Dissipation; Dynamic compression; Mechanobiology; Scaffold