title: Application of 13C isotope labeling using liquid chromatography mass spectrometry (LC-MS) to determining phosphate-containing metabolic incorporation.
abstract: Here, we describe an approach wherein negative electrospray ionization mass spectrometry has used to understand the relative flux through phosphate containing metabolic intermediates associated with central carbon metabolism after administering cells with 13C-labeled substrates. The method was applied to examine the 13C incorporation through glycolysis in T47D breast cancer cells and showed reduction of glycolytic relative flux upon treatment with 2-Deoxyglucose.
authors: Department of Molecular and Cell Biology, Baylor College of Medicine, Houston, TX, 77030, USA; Alkek Center for Molecular Discovery, Baylor College of Medicine, Houston, TX, 77030, USA.
authors: Salil Kumar
authors: SK
authors: Bhowmik
authors: Vasanta
authors: V
authors: Putluri
authors: Ramakrishna
authors: R
authors: Kommagani
authors: Sai Aparna
authors: SA
authors: Konde
authors: John P
authors: JP
authors: Lydon
authors: Arun
authors: A
authors: Sreekumar
authors: Nagireddy
authors: N
authors: Putluri
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism; D002247:Carbon Isotopes / Q000032:analysis / Q000378:metabolism*; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D006019:Glycolysis / Q000502:physiology; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D064688:Metabolic Flux Analysis / Q000379:methods*; D010710:Phosphates / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry
chemical_list: D002247:Carbon Isotopes; D010710:Phosphates
keywords: metabolic relative flux; single reaction monitoring; tandem mass spectrometry