title: Hypermethylation of EDNRB promoter contributes to the risk of colorectal cancer.
abstract: OBJECTIVE
  Colorectal cancer (CRC) is one of the most common digestive malignancies in the world. EDNRB is a new candidate tumor suppressor gene which is often down-regulated or even silenced by promoter hypermethylation in various human cancers. However, the function of EDNRB gene in CRC remains unknown. In this study, we examined the expression and DNA methylation of EDNRB in CRC tissues.
  
  
  METHODS
  A total of 42 paired CRC and adjacent normal tissue samples were used to determine mRNA levels and DNA methylation status of EDNRB gene by qRT-PCR and methylation-specific PCR (MSP), respectively.
  
  
  RESULTS
  Our study showed that EDNRB promoter hypermethylation was more frequently in CRC tissues than in the normal tissues (92.86 versus 59.52, p = 0.001). Consequently, significantly lower level of EDNRB mRNA was found in CRC tumor samples than in normal samples (0.31 ± 0.91 versus 0.70 ± 1.18, p = 0.032).
  
  
  CONCLUSIONS
  Our results suggested that EDNRB promoter hypermethylation might downregulate its gene expression in CRC, and thus played an important role in the development of CRC.
  The virtual slides for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/7420980471113303.
authors: Cheng
authors: C
authors: Chen
authors: Lingyan
authors: L
authors: Wang
authors: Qi
authors: Q
authors: Liao
authors: Yi
authors: Y
authors: Huang
authors: Huadan
authors: H
authors: Ye
authors: Fei
authors: F
authors: Chen
authors: Leiting
authors: L
authors: Xu
authors: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China. yemeng@nbu.edu.cn.
authors: Meng
authors: M
authors: Ye
authors: Shiwei
authors: S
authors: Duan
mesh_terms: D044466:Asian People / Q000235:genetics; D002681:China; D003106:Colon / Q000378:metabolism / Q000473:pathology; D015179:Colorectal Neoplasms / Q000453:epidemiology* / Q000235:genetics* / Q000378:metabolism; D019175:DNA Methylation / Q000235:genetics*; D015536:Down-Regulation / Q000502:physiology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin / Q000235:genetics* / Q000378:metabolism; D012189:Retrospective Studies; D012307:Risk Factors
chemical_list: C000588596:EDNRB protein, human; D012333:RNA, Messenger; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin