title: Small RNA analysis of Potato spindle tuber viroid infected Phelipanche ramosa.
abstract: Plants defend themselves against virus/viroid infection by induction of a mechanism of viral RNA degradation or translation inhibition. This is achieved by the production of small RNAs referred to as small interfering RNAs and microRNA, the key molecules in establishment of RNA directed silencing. Potato Spindle Tuber Viroid (PSTVd) was the first viroid species to be identified as naturally infecting potato, and it was found to infect many other crop species, wild and ornamental plants. Recently the experimental host range of PSTVd was extended with the root non-photosynthetic parasitic weed--Phelipanche ramosa (L) Pomel. Here we examined the small RNA population in P. ramosa infected with PSTVd and we observed the presence of PSTVd derived small RNAs. The hotspot regions for production of those PSTVd specific small RNAs were defined by their mapping on the viroid genome sequence. Further, we evaluated the expression levels of selected conserved microRNA families in the viroid infected P. ramosa. Upon infection, two members of miRNA395 family were significantly accumulated, while several members of miRNA390, miRNA396, miRNA319, miRNA166, miRNA167 and miRNA159 were strongly down-regulated. All these findings imply the involvement of various small RNA classes in the P. ramosa response to PSTVd infection.
authors: Department of Plant Physiology and Molecular Biology, University of Plovdiv 24, Tsar Assen Str., 4000 Plovdiv, Bulgaria; IMBB FORTH, Nikolau Plastira 100, P.O. Box 1385, GR-70013 Heraklion, Crete, Greece; Biology Department, University of Crete, Greece, Vasilika Vouton, P.O. Box 2208, 71409 Heraklion, Crete, Greece.
authors: Desislava
authors: D
authors: Ivanova
authors: Department of Plant Physiology and Molecular Biology, University of Plovdiv 24, Tsar Assen Str., 4000 Plovdiv, Bulgaria.
authors: Ivan
authors: I
authors: Milev
authors: Department of Plant Physiology and Molecular Biology, University of Plovdiv 24, Tsar Assen Str., 4000 Plovdiv, Bulgaria.
authors: Tihomir
authors: T
authors: Vachev
authors: Department of Plant Physiology and Molecular Biology, University of Plovdiv 24, Tsar Assen Str., 4000 Plovdiv, Bulgaria.
authors: Vesselin
authors: V
authors: Baev
authors: Department of Plant Physiology and Molecular Biology, University of Plovdiv 24, Tsar Assen Str., 4000 Plovdiv, Bulgaria.
authors: Galina
authors: G
authors: Yahubyan
authors: Department of Plant Physiology and Molecular Biology, University of Plovdiv 24, Tsar Assen Str., 4000 Plovdiv, Bulgaria.
authors: Georgi
authors: G
authors: Minkov
authors: Department of Plant Physiology and Molecular Biology, University of Plovdiv 24, Tsar Assen Str., 4000 Plovdiv, Bulgaria. Electronic address: mgozmanova@gmail.com.
authors: Mariyana
authors: M
authors: Gozmanova
mesh_terms: D015152:Blotting, Northern; D035683:MicroRNAs / Q000235:genetics; D031670:Orobanchaceae / Q000235:genetics / Q000821:virology*; D010942:Plant Viruses / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D018749:RNA, Plant / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant
keywords: PSTVd; PSTVd sRNAs; Phelipanche ramosa; miRNAs