title: Cell damage induced by copper: an explant model to study anemone cells.
abstract: Sea anemones are benthic organisms, of low mobility and can be directly affected by water pollution. This work studied the defense mechanisms and DNA damage caused by copper toxicity in cells from the anemone Bunodosoma cangicum. For this, exposure of anemones cells were held, kept in primary culture through explant of podal disk to copper (7.8 and 15.6 μg/L), and the control group, for 6 and 24h. Cytotoxicity was seen through the viability and cell number, MXR phenotype through the accumulation of rhodamine-B, ROS generation by H2DCF-DA and DNA damage by comet assay. The results obtained show that there is a drop in viability and number of cells, especially after exposure of 24h in 15.6 μg/L. There is an induction of the MXR activity only at 7.8 μg/L for 24h. As for ROS, there is an increase in the generation of reactive species in greatest concentration of copper for 6h, and in both for 24h, which leads to oxidative stress, which culminates with a DNA damage. What was evidenced by the increase of the tail size, % DNA presented and moment of tail. Therefore, the copper represents an adversity to the anemones cells, being cytotoxic and genotoxic.
authors: Programa de Pós-Graduação em Ciências Fisiológicas, Fisiologia Animal Comparada, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Av. Itália km 8, 96.203-900, Rio Grande, Rio Grande do Sul, Brazil.
authors: Vanessa A
authors: VA
authors: Anjos
authors: Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Rio Grande, Rio Grande do Sul, Brazil.
authors: Flávio M R
authors: FM
authors: da Silva
authors: Programa de Pós-Graduação em Ciências Fisiológicas, Fisiologia Animal Comparada, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Av. Itália km 8, 96.203-900, Rio Grande, Rio Grande do Sul, Brazil; Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Rio Grande, Rio Grande do Sul, Brazil. Electronic address: martasouza@furg.br.
authors: Marta M
authors: MM
authors: Souza
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D003300:Copper / Q000008:administration & dosage / Q000633:toxicity*; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D009152:Mutagenicity Tests; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012615:Sea Anemones / Q000166:cytology / Q000187:drug effects*; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D009153:Mutagens; D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; D003300:Copper
keywords: Anemone’s cell; Copper; Cytotoxicity; MXR; ROS and DNA damage