title: Does the S phase have an impact on the accuracy of comparative genomic hybridization profiles in single fibroblasts and human blastomeres?
abstract: OBJECTIVE
  To investigate if there is an association between single-cell replicative stage and the segmental chromosome imbalances detected by comparative genomic hybridization (CGH).
  
  
  METHODS
  First, 135 fibroblasts from cell-line GM03184 (Coriell) at three cell stages (G0/G1, S, and G2/M) were amplified by degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) or Sureplex and blindly analyzed by CGH. Second, 85 human blastomeres at the interphase and the metaphase stages, from 30 donated human cryopreserved embryos, were amplified by Sureplex and analyzed by CGH.
  
  
  METHODS
  Academic center for reproductive medicine.
  
  
  METHODS
  None.
  
  
  METHODS
  None.
  
  
  METHODS
  Incidence of aneuploidy and segmental imbalances detected at the different cell stages.
  
  
  RESULTS
  In DOP-PCR amplifications of fibroblasts, an increased incidence of segmental abnormalities was detected in the S phase. In Sureplex amplifications of fibroblasts and blastomeres, no differences were detected between the different cell stages. A significantly increased incidence of structural abnormalities was seen in the aneuploid blastomeres.
  
  
  CONCLUSIONS
  The segmental imbalances detected after Sureplex amplification in 73.3% of the cryopreserved embryos analyzed are mainly nontransitory. They correspond to segmental imbalances present in the cells due to chromosome instability, rather than to replicative DNA segments.
authors: Unitat de Biologia Cel·lular i Genètica Mèdica. Facultat de Medicina, Departament de Biologia Cel·lular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors: Laia
authors: L
authors: Ramos
authors: Unitat de Biologia Cel·lular i Genètica Mèdica. Facultat de Medicina, Departament de Biologia Cel·lular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors: Javier
authors: J
authors: del Rey
authors: Unitat de Biologia Cel·lular i Genètica Mèdica. Facultat de Medicina, Departament de Biologia Cel·lular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors: Gemma
authors: G
authors: Daina
authors: Fundació Puigvert, Hospital de Sant Pau i de la Santa Creu, Barcelona, Spain.
authors: Olga
authors: O
authors: Martinez-Passarell
authors: Clínica Sagrada Família, Barcelona, Spain.
authors: Mariona
authors: M
authors: Rius
authors: Institut Universitari Dexeus, Barcelona, Spain.
authors: Dolores
authors: D
authors: Tuñón
authors: Àrea de Medicina Preventiva i de Salut Pública. Facultat de Medicina. Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors: Mercedes
authors: M
authors: Campillo
authors: Unitat de Biologia Cel·lular i Genètica Mèdica. Facultat de Medicina, Departament de Biologia Cel·lular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors: Jordi
authors: J
authors: Benet
authors: Unitat de Biologia Cel·lular i Genètica Mèdica. Facultat de Medicina, Departament de Biologia Cel·lular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, Bellaterra, Spain. Electronic address: joaquima.navarro@uab.cat.
authors: Joaquima
authors: J
authors: Navarro
mesh_terms: D001757:Blastomeres / Q000502:physiology*; D002460:Cell Line; D055028:Comparative Genomic Hybridization / Q000379:methods* / Q000592:standards*; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D005434:Flow Cytometry / Q000379:methods / Q000592:standards; D006801:Humans; D016196:S Phase / Q000502:physiology*; D016037:Single-Blind Method
keywords: CGH; Cell cycle; aneuploidy; human cryopreserved embryos; segmental imbalances