title: Detection of acute toxoplasmosis in pigs using loop-mediated isothermal amplification and quantitative PCR.
abstract: A loop-mediated isothermal amplification (LAMP) assay allows rapid diagnosis of Toxoplasma gondii infection. In the present study, the LAMP assay was evaluated using blood from both naturally and experimentally infected pigs. The sensitivity of the LAMP assay was compared with that of Q-PCR. Both assays detected T. gondii in the blood of experimentally infected pigs, with 100% agreement. In infected blood samples, the parasite was detected as early as 2 days post-infection and reached a peak in 3-5 days. In 216 field serum samples, the detection rates of LAMP and Q-PCR assays were 6.9% and 7.8%, respectively. This result indicates that the sensitivity of the LAMP assay was slightly lower than that of the Q-PCR assay. However, the LAMP may be an attractive diagnostic method in conditions where sophisticated and expensive equipment is unavailable. This assay could be a powerful supplement to current diagnostic methods.
authors: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors: Yanhua
authors: Y
authors: Wang
authors: Guangxiang
authors: G
authors: Wang
authors: Delin
authors: D
authors: Zhang
authors: Hong
authors: H
authors: Yin
authors: Meng
authors: M
authors: Wang
mesh_terms: D000818:Animals; D001399:Azure Stains; D001681:Biological Assay; D001921:Brain / Q000469:parasitology; D016054:DNA, Protozoan / Q000097:blood* / Q000235:genetics; D008168:Lung / Q000469:parasitology; D051379:Mice; D021141:Nucleic Acid Amplification Techniques / Q000662:veterinary*; D018512:Parasitemia; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000469:parasitology; D014122:Toxoplasma / Q000235:genetics / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D001399:Azure Stains; D016054:DNA, Protozoan
keywords: Q-PCR; Toxoplasma gondii; loop-mediated isothermal amplification (LAMP); pig