title: TRAPID: an efficient online tool for the functional and comparative analysis of de novo RNA-Seq transcriptomes.
abstract: Transcriptome analysis through next-generation sequencing technologies allows the generation of detailed gene catalogs for non-model species, at the cost of new challenges with regards to computational requirements and bioinformatics expertise. Here, we present TRAPID, an online tool for the fast and efficient processing of assembled RNA-Seq transcriptome data, developed to mitigate these challenges. TRAPID offers high-throughput open reading frame detection, frameshift correction and includes a functional, comparative and phylogenetic toolbox, making use of 175 reference proteomes. Benchmarking and comparison against state-of-the-art transcript analysis tools reveals the efficiency and unique features of the TRAPID system. TRAPID is freely available at http://bioinformatics.psb.ugent.be/webtools/trapid/.
authors: Michiel
authors: M
authors: Van Bel
authors: Sebastian
authors: S
authors: Proost
authors: Christophe
authors: C
authors: Van Neste
authors: Dieter
authors: D
authors: Deforce
authors: Yves
authors: Y
authors: Van de Peer
authors: Klaas
authors: K
authors: Vandepoele
mesh_terms: D019295:Computational Biology / Q000379:methods; D019143:Evolution, Molecular; D020869:Gene Expression Profiling / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D010802:Phylogeny; D017423:Sequence Analysis, RNA / Q000379:methods*; D064878:Web Browser