title: Carbohydrate analysis: from sample preparation to HPLC on different stationary phases coupled with evaporative light-scattering detection.
abstract: After 20 years of development, evaporative light-scattering detection (ELSD) has become the mainstream choice for the detection of various classes of natural products. ELSD continues to grow in popularity as a "quasi-universal" technique because of the specificity of the detection method, which is based on the scattering of laser light from nonvolatile analyte particles. It represents an attractive alternative compared to other types of detection, such as refractive index detection and/or ultraviolet detection. This review presents issues concerned with the separation of carbohydrates in plant materials by HPLC and ELSD, as well as the advantages and limitations relating to the ELSD method. Additionally, an overview of possible ELSD applications in the analysis of carbohydrates in natural products is presented.
authors: Department of Chemistry and Biochemistry, Mendel University in Brno, Czech Republic.
authors: Eva
authors: E
authors: Dvořáčková
authors: Marie
authors: M
authors: Snóblová
authors: Petr
authors: P
authors: Hrdlička
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D008027:Light; D012542:Scattering, Radiation; D014835:Volatilization
chemical_list: D002241:Carbohydrates
keywords: Evaporative light-scattering detection; HPLC; Saccharides; Stationary phase