title: Gold nanoparticle loaded hybrid nanofibers for cardiogenic differentiation of stem cells for infarcted myocardium regeneration.
abstract: Heart disease is the leading cause of mortality in many industrialized nations and is often related to irregularities in electrical function that can radically damage cardiac functioning. The aim of this study is to develop a novel therapeutic hybrid scaffold that can couple electrical, mechanical, and biological properties, desirable for cardiac tissue regeneration. BSA/PVA scaffolds are fabricated in the ratio 2:1 and gold nanoparticles (AuNPs) embedded scaffolds in the ratios BSA/PVA/Au of 2:1:0.1 (lower concentration) and BSA/PVA/Au of 2:1:0.4 (higher concentration) by electrospinning. The scaffolds are characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), contact angle, Fourier transform infrared (FTIR) spectroscopy, and tensile testing to analyze the fiber morphology, AuNP distribution, hydrophilicity, surface functional groups, and mechanical properties of the scaffolds, respectively. Results show that ex vivo pretreatment of MSCs using 5-aza and AuNPs loaded conductive nanofibrous construct could lead to enhanced cardiomyogenic differentiation and result in superior biological and functional effects on infarcted myocardium regeneration.
authors: Healthcare and Energy Materials Laboratory, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, Singapore; Department of Mechanical Engineering, National University of Singapore, Singapore, 117576, Singapore.
authors: Rajeswari
authors: R
authors: Ravichandran
authors: Radhakrishnan
authors: R
authors: Sridhar
authors: Jayarama Reddy
authors: JR
authors: Venugopal
authors: Subramanian
authors: S
authors: Sundarrajan
authors: Shayanti
authors: S
authors: Mukherjee
authors: Seeram
authors: S
authors: Ramakrishna
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation / Q000235:genetics; D006046:Gold / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D009203:Myocardial Infarction / Q000473:pathology / Q000628:therapy; D009206:Myocardium / Q000473:pathology*; D057139:Nanofibers / Q000008:administration & dosage / Q000737:chemistry; D012038:Regeneration; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology; D023822:Tissue Engineering
chemical_list: D006046:Gold
keywords: biomaterials; heart failure; in vitro studies; myocardial infarction; tissue engineering