title: Wide-field two-dimensional multifocal optical-resolution photoacoustic-computed microscopy.
abstract: Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technique that directly images optical absorption in tissue at high spatial resolution. To date, the majority of OR-PAM systems are based on single-focused optical excitation and ultrasonic detection, limiting the wide-field imaging speed. While 1D multifocal OR-PAM (1D-MFOR-PAM) has been developed, the potential of microlens and transducer arrays has not been fully realized. Here we present the development of 2D multifocal optical-resolution photoacoustic-computed microscopy (2D-MFOR-PACM), using a 2D microlens array and a full-ring ultrasonic transducer array. The 10 mm×10 mm microlens array generates 1800 optical foci within the focal plane of the 512-element transducer array, and raster scanning the microlens array yields optical-resolution photoacoustic images. The system has improved the in-plane resolution of a full-ring transducer array from ≥100 to 29 μm and achieved an imaging time of 36 s over a 10 mm×10 mm field of view. In comparison, the 1D-MFOR-PAM would take more than 4 min to image over the same field of view. The imaging capability of the system was demonstrated on phantoms and animals both ex vivo and in vivo.
authors: Jun
authors: J
authors: Xia
authors: Guo
authors: G
authors: Li
authors: Lidai
authors: L
authors: Wang
authors: Mohammadreza
authors: M
authors: Nasiriavanaki
authors: Konstantin
authors: K
authors: Maslov
authors: John A
authors: JA
authors: Engelbach
authors: Joel R
authors: JR
authors: Garbow
authors: Lihong V
authors: LV
authors: Wang
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D005260:Female; D051379:Mice; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D055620:Optical Phenomena; D061088:Photoacoustic Techniques / Q000295:instrumentation / Q000379:methods*; D011247:Pregnancy; D014159:Transducers; D014599:Uterus / Q000166:cytology