title: Role of the cytosolic tails of Rift Valley fever virus envelope glycoproteins in viral morphogenesis.
abstract: The correct folding, heterodimerization and trafficking of Gn/Gc envelope glycoproteins of Rift Valley fever virus, RVFV (Bunyaviridae and Phlebovirus genus) are essential for Golgi assembly and budding of viral particles. The Gn and Gc carboxy-terminus contain a Golgi targeting and an ER-retrieval signal, respectively. We generated RVFV-like particles with mutations in the cytosolic tails of Gn or Gc and identified regions important for release of infectious particles. The role of specific amino-acids in these regions was further investigated by creating recombinant mutant viruses by reverse-genetics. Residues outside the suspected Golgi targeting motif, i.e. the di-lysine K29-K30 motif and the N43, R44 and I46 residues of the Gn cytosolic domain, appeared important for Golgi localization and RNP packaging. Concerning the Gc tail, replacement of K2 or K3 in the di-lysine motif, had a drastic impact on Gn trafficking and induced an important organelle redistribution and cell remodeling, greatly affecting particle formation and release.
authors: Institut Pasteur, Unité de Génétique Moléculaire des Bunyavirus, 25 rue du Docteur Roux, 75015 Paris, France.
authors: Xavier
authors: X
authors: Carnec
authors: Myriam
authors: M
authors: Ermonval
authors: Felix
authors: F
authors: Kreher
authors: Marie
authors: M
authors: Flamand
authors: Michèle
authors: M
authors: Bouloy
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003600:Cytosol / Q000821:virology; D006056:Golgi Apparatus / Q000821:virology; D006801:Humans; D008969:Molecular Sequence Data; D012295:Rift Valley Fever / Q000821:virology*; D012296:Rift Valley fever virus / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D014759:Viral Envelope Proteins
keywords: Arbovirus; Bunyaviridae; Cytoskeleton; Golgi; Phlebovirus; Viral morphogenesis