title: [Construction and characterization of EGFP reporter gene labeled Sindbis virus].
abstract: OBJECTIVE
  To construct and characterize EGFP reporter gene labeled Sindbis virus (SINV).
  
  
  METHODS
  The reporter gene EGFP was inserted into the genome of infectious clone pBR-XJ160 by using multi-fusion long fragment PCR method. Then apply reverse genetic manipulation technique to rescue and obtain EGFP labeled SINV.
  
  
  RESULTS
  We successively obtained labeled SINV, which has good fluorescent expression characteristics and genetic stability.
  
  
  CONCLUSIONS
  The labeled virus can be seen in living cells and living body, and this serves as a good tool for cell and tissue tropism and biological function study of viruses. This study laid a foundation for further studying the cell tropism, biological functions and infection mechanism of SINV.
authors: College of Life Science & Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors: Ling-Ling
authors: LL
authors: Deng
authors: Jiang-Jiao
authors: JJ
authors: Li
authors: Yan
authors: Y
authors: Wei
authors: Huan-Qin
authors: HQ
authors: Wang
authors: Feng-Juan
authors: FJ
authors: Zhang
authors: Ji-Guo
authors: JG
authors: Sun
authors: Chang
authors: C
authors: Chen
authors: Wu-Yang
authors: WY
authors: Zhu
authors: Guo-Dong
authors: GD
authors: Liang
mesh_terms: D001483:Base Sequence; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D012845:Sindbis Virus / Q000235:genetics*
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins