title: Immunoglobulin G immobilization on PVDF surface.
abstract: Immobilization of antibody molecules onto hydrophobic polymeric surfaces with disordered orientation is something unwanted in many applications. To overcome this drawback, controlled immunoglobulin G (IgG) immobilization onto poly(vinylidene fluoride) surface was investigated in this paper. A two-step process involving radiofrequency plasma pretreatment for polymer surface functionalization, followed by coupling reaction was developed, after which immunoglobulin G was immobilized onto the surface directly or via protein-A. IR and XPS data proved that the process is more efficient when the radiofrequency plasma pretreatment was performed using N2 and N2/H2 as discharge gases. NIR-CI, AFM and XPS surface evaluation revealed that immobilization of IgG onto N2/H2 plasma-treated PVDF via grafted protein-A was achieved with an ends-on orientation, leaving available the antigen binding sites of IgG. This procedure could be a promising route for the preparation of oriented IgG assembly onto PVDF, useful in biomedical, membranes or sensors applications. QCM results showed a better antibody-antigen interaction when IgG immobilization onto PVDF substrate is mediated by protein A.
authors: "Petru Poni" Institute of Macromolecular Chemistry, Physical Chemistry of Polymers Department, 41A Gr. Ghica Voda Alley, 700487 Iasi, Romania.
authors: Elena
authors: E
authors: Pâslaru
authors: "Gr. T. Popa" Medicine and Pharmacy University, 16, University Street, 700115 Iasi, Romania.
authors: Mihaela Cristina
authors: MC
authors: Baican
authors: "Petru Poni" Institute of Macromolecular Chemistry, Physical Chemistry of Polymers Department, 41A Gr. Ghica Voda Alley, 700487 Iasi, Romania.
authors: Elena Gabriela
authors: EG
authors: Hitruc
authors: "Petru Poni" Institute of Macromolecular Chemistry, Physical Chemistry of Polymers Department, 41A Gr. Ghica Voda Alley, 700487 Iasi, Romania.
authors: Manuela Tatiana
authors: MT
authors: Nistor
authors: Laboratoire Polymères, Colloides et Interfaces, UMR 6120 CNRS-Université du Maine av. O. Messiaen, 72000 Le Mans, France.
authors: Fabienne
authors: F
authors: Poncin-Epaillard
authors: "Petru Poni" Institute of Macromolecular Chemistry, Physical Chemistry of Polymers Department, 41A Gr. Ghica Voda Alley, 700487 Iasi, Romania. Electronic address: cvasile@icmpp.ro.
authors: Cornelia
authors: C
authors: Vasile
mesh_terms: D000941:Antigens / Q000378:metabolism; D016002:Discriminant Analysis; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*; D016018:Least-Squares Analysis; D018625:Microscopy, Atomic Force; D056951:Photoelectron Spectroscopy; D011145:Polyvinyls / Q000737:chemistry*; D025341:Principal Component Analysis; D011485:Protein Binding; D058525:Quartz Crystal Microbalance Techniques; D017550:Spectroscopy, Fourier Transform Infrared; D019265:Spectroscopy, Near-Infrared; D013205:Staphylococcal Protein A / Q000378:metabolism; D013499:Surface Properties; D005465:Fluorocarbon Polymers
chemical_list: D000941:Antigens; D055767:Immobilized Proteins; D007074:Immunoglobulin G; D011145:Polyvinyls; D013205:Staphylococcal Protein A; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers
keywords: Immunoglobulin G; PVDF; Plasma; Protein A; Surface functionalization