title: Aggregated chromosomes transfer in human oocytes.
abstract: Germinal-vesicle (GV) transfer, spindle-chromosome complex transfer in metaphase-II oocytes and two pronuclei transfer have been evaluated as possible treatments for patients who have mitochondrial diseases. However, GV transfers often lead to heteroplasmy while the other two methods are frequently associated with aneuploidy. The present study used a new method based on the transfer of aggregated chromosomes, which occurs in human oocytes, before the metaphase spindle is established.
authors: Nagai Clinic, 607-1 Kamihikona, Misato, Saitama 341-0004, Japan. Electronic address: otsuki.midori.junko@gmail.com.
authors: Junko
authors: J
authors: Otsuki
authors: Nagai Clinic, 607-1 Kamihikona, Misato, Saitama 341-0004, Japan.
authors: Yasushi
authors: Y
authors: Nagai
authors: Tsukuba Primate Research Center, National Institute of Biomedical Innovation, Japan.
authors: Tadashi
authors: T
authors: Sankai
mesh_terms: D002467:Cell Nucleus / Q000648:ultrastructure; D002877:Chromosomes, Human; D006801:Humans; D008540:Meiosis; D008677:Metaphase; D028361:Mitochondrial Diseases / Q000235:genetics*; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000648:ultrastructure; D027724:Reproductive Techniques, Assisted; D008941:Spindle Apparatus / Q000648:ultrastructure; D059008:Time-Lapse Imaging
keywords: aggregated chromosomes; aneuploidy; heteroplasmy; microinjection; mitochondrial disease; nuclear transfer