title: [Effect of ropivacaine on proliferation and migration of rat bone marrow mesenchymal stem cells].
abstract: OBJECTIVE
  To observe the influence of ropivacaine on the proliferation and migration of rat bone marrow mesenchymal stem cells (BMSCs) and provide basis for the clinical application of BMSCs.
  
  
  METHODS
  Rat BMSCs were isolated and cultured by adherence method. Surface markers of BMSCs were examined by flow cytometry. Multipotent differentiation of BMSCs was detected by induced adipogenesis, osteogenesis and muscular differentiation. Proliferation of BMSCs was examined by CCK-8 and Brdu incorporation after ropivacaine treatment at different concentrations. Migration of BMSCs was tested by cell scratch assay and Millicell experiment.
  
  
  RESULTS
  Cultured cells had representative appearance and surface markers of BMSC, and they had potential multiple differentiation. Ropivacaine treatment at 50 and 100 μmol/L significantly reduced the proliferation rate of BMSCs and Brdu incorporation rate. There was significant difference compared with the control group (P<0.05). Cellular scratch assay and migration experiment indicated that ropivacaine significantly reduced the migration of BMSCs. There was significant difference compared with the control group (P<0.05). All these mentioned effects of ropivacaine on BMSCs were dose-dependent. There was significant difference between groups (P<0.05).
  
  
  CONCLUSIONS
  Ropivacaine can significantly reduce the proliferation and migration of rat BMSCs, suggesting that the influence of local anesthetics on BMSCs has to be taken into account when BMSCs are used in clinical practice.
authors: Department of Physiology, Changzhi Medical College, Changzhi Shanxi 046000, China guoxiaoshu999@163.com.
authors: Xiaoshu
authors: X
authors: Guo
authors: Jiping
authors: J
authors: Gong
authors: Genqing
authors: G
authors: Yang
authors: Yongli
authors: Y
authors: Chang
authors: Lijing
authors: L
authors: Gao
authors: Xiaoxia
authors: X
authors: Tian
mesh_terms: D000577:Amides / Q000494:pharmacology*; D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005434:Flow Cytometry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D000077212:Ropivacaine
chemical_list: D000577:Amides; D000077212:Ropivacaine