title: Differential gene expression of cardiac ion channels in human dilated cardiomyopathy.
abstract: BACKGROUND
  Dilated cardiomyopathy (DCM) is characterized by idiopathic dilation and systolic contractile dysfunction of the cardiac chambers. The present work aimed to study the alterations in gene expression of ion channels involved in cardiomyocyte function.
  
  
  RESULTS
  Microarray profiling using the Affymetrix Human Gene® 1.0 ST array was performed using 17 RNA samples, 12 from DCM patients undergoing cardiac transplantation and 5 control donors (CNT). The analysis focused on 7 cardiac ion channel genes, since this category has not been previously studied in human DCM. SCN2B was upregulated, while KCNJ5, KCNJ8, CLIC2, CLCN3, CACNB2, and CACNA1C were downregulated. The RT-qPCR (21 DCM and 8 CNT samples) validated the gene expression of SCN2B (p < 0.0001), KCNJ5 (p < 0.05), KCNJ8 (p < 0.05), CLIC2 (p < 0.05), and CACNB2 (p < 0.05). Furthermore, we performed an IPA analysis and we found a functional relationship between the different ion channels studied in this work.
  
  
  CONCLUSIONS
  This study shows a differential expression of ion channel genes involved in cardiac contraction in DCM that might partly underlie the changes in left ventricular function observed in these patients. These results could be the basis for new genetic therapeutic approaches.
authors: Cardiocirculatory Unit, Health Research Institute Hospital La Fe, Valencia, Spain.
authors: Maria Micaela
authors: MM
authors: Molina-Navarro
authors: Esther
authors: E
authors: Roselló-Lletí
authors: Ana
authors: A
authors: Ortega
authors: Estefanía
authors: E
authors: Tarazón
authors: Manuel
authors: M
authors: Otero
authors: Luis
authors: L
authors: Martínez-Dolz
authors: Francisca
authors: F
authors: Lago
authors: José Ramón
authors: JR
authors: González-Juanatey
authors: Francisco
authors: F
authors: España
authors: Pablo
authors: P
authors: García-Pavía
authors: José Anastasio
authors: JA
authors: Montero
authors: Manuel
authors: M
authors: Portolés
authors: Miguel
authors: M
authors: Rivera
mesh_terms: D002311:Cardiomyopathy, Dilated / Q000235:genetics* / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007473:Ion Channels / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D007473:Ion Channels