title: Differential upregulation of human 2'5'OAS genes on systemic sclerosis: Detection of increased basal levels of OASL and OAS2 genes through a qPCR based assay.
abstract: 2'5'OAS are template-independent RNA polymerases with antiviral activity and important to homeostasis maintenance. Here we have developed quantitative PCR (qPCR) reactions for the detection of each individual 2'5'OAS human gene and used them to evaluate these gene levels in systemic sclerosis patients cells. The method was efficient for quantification of 2'5'OAS genes on human cells after interferon (IFN) treatment, and revealed that primary cells from patients with systemic sclerosis have increased basal levels of OASL and OAS2 genes. When treated, patients cells are able to induce all four 2'5'OAS genes. Our hypothesis is that abnormally circulating type I IFNs on the disease could be establishing a desensitized state on patients cells, making them refractory to subsequent IFN doses, and that OASL and OAS2 genes upregulation may be due to an IFN-independent stimulus. Further characterizing the biological activities of these genes, their induction pathways and their regulatory functions can lead to better understanding of systemic sclerosis molecular mechanisms and of their biological activities.
authors: Laboratório de Vírus, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais , Belo Horizonte, Minas Gerais , Brazil and.
authors: Gabriel Magno
authors: GM
authors: de Freitas Almeida
authors: Danilo Bretas de
authors: DB
authors: Oliveira
authors: Lucas Moreira
authors: LM
authors: Botelho
authors: Ludmila Karen dos Santos
authors: LK
authors: Silva
authors: Antônio Carlos Martins
authors: AC
authors: Guedes
authors: Flávia Patrícia Sena Teixeira
authors: FP
authors: Santos
authors: Cláudio Antônio
authors: CA
authors: Bonjardim
authors: Paulo César Peregrino
authors: PC
authors: Ferreira
authors: Erna Geessien
authors: EG
authors: Kroon
mesh_terms: D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics* / Q000276:immunology; D000328:Adult; D000368:Aged; D045744:Cell Line, Tumor; D005260:Female; D015870:Gene Expression; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology; D016899:Interferon-beta / Q000494:pharmacology; D007527:Isoenzymes / Q000235:genetics / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009928:Organ Specificity; D016133:Polymerase Chain Reaction / Q000379:methods*; D061251:Primary Cell Culture; D012595:Scleroderma, Systemic / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D015854:Up-Regulation
chemical_list: D016898:Interferon-alpha; D007527:Isoenzymes; D016899:Interferon-beta; C115020:OASL protein, human; D015088:2',5'-Oligoadenylate Synthetase