title: Compared analysis of LncRNA expression profiling in pdk1 gene knockout mice at two time points.
abstract: OBJECTIVE
  Previous studies have indicated that long non-coding RNAs (lncRNA) are related to the occurrence and development of many human diseases, such as cancer and the HELLP and the brachydactyly syndromes. However, studies of LncRNA in heart failure have not yet been reported. Here, we investigated cardiac lncRNA expression profiles in the myocardial-specific knockout pdk1 gene (KO) mouse model of heart failure.
  
  
  METHODS
  Cardiac samples were obtained from PDK1 KO and WT mice on postnatal (P) day 8 (P8) and day 40 (P40), and lncRNA expression profiles were analyzed by sequencing and screening using the Arraystar mouse lncRNA microarray. Quantitative real-time PCR analysis of these lncRNAs confirmed the identity of some genes.
  
  
  RESULTS
  Comparisons of the KO and control groups showed fold changes of >1.5 in the expression levels of 2,024 lncRNAs at P8, while fold changes of >2 in the expression levels of 4,095 lncRNAs were detected at P40. Nineteen lncRNAs were validated by RT-PCR. Bioinformatic and pathway analyses indicated that mkk7, a sense overlap lncRNA, may be involved in the pathological processes of heart failure through the MAPK signaling pathway.
  
  
  CONCLUSIONS
  These data reveal differentially expressed lncRNA in mice with a myocardial-specific deletion of the pdk1 gene, which may provide new insights into the mechanism of heart failure in PDK1 knockout mice.
authors: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, P. R. China.
authors: Hailang
authors: H
authors: Liu
authors: Guixian
authors: G
authors: Song
authors: Lijuan
authors: L
authors: Zhou
authors: Xiaoshan
authors: X
authors: Hu
authors: Ming
authors: M
authors: Liu
authors: Junwei
authors: J
authors: Nie
authors: Shuangshuang
authors: S
authors: Lu
authors: Xiangqi
authors: X
authors: Wu
authors: Yunshan
authors: Y
authors: Cao
authors: Lichan
authors: L
authors: Tao
authors: Ling
authors: L
authors: Chen
authors: Lingmei
authors: L
authors: Qian
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D006333:Heart Failure / Q000235:genetics*; D048688:MAP Kinase Kinase 7 / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D062085:RNA, Long Noncoding; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results
chemical_list: C000631429:PDK1 protein, human; C000631433:Pdk1 protein, mouse; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D062085:RNA, Long Noncoding; D017346:Protein Serine-Threonine Kinases; D048688:MAP Kinase Kinase 7; C482570:Map2k7 protein, mouse