title: MicroRNA-100 inhibits osteosarcoma cell proliferation by targeting Cyr61.
abstract: Increasing evidence indicates that microRNAs (miRNAs) participate in almost every step of cellular processes and are often aberrantly expressed in human cancer. Therefore, the discovery of miRNAs may provide a new and powerful tool for understanding the mechanism and treatment of carcinogenesis. The aim of this study was to investigate the functional significance of miR-100 and to identify its possible target genes in osteosarcoma (OS) cells. Here, we found that expression level of miR-100 was significantly decreased in osteosarcoma tissues in comparison with the adjacent normal tissues. The enforced expression of miR-100 was able to inhibit cell proliferation in Saos-2 and MG63 cells, while its antisense oligonucleotides (antisense miR-100) promoted cell proliferation. Moreover, our results further revealed that expression of Cyr61, an extracellular matrix-associated growth factor, was negatively regulated by miR-100. Therefore, we consider that miR-100 acts as a tumor suppressor for osteosarcoma. It may provide novel diagnostic and therapeutic options for human osteosarcoma in the future.
authors: Department of Orthopaedics, Affiliated People's First Hospital, Shanghai Jiaotong University, 200080, Shanghai, China.
authors: Jianhua
authors: J
authors: Huang
authors: Kanda
authors: K
authors: Gao
authors: Jian
authors: J
authors: Lin
authors: Qiugen
authors: Q
authors: Wang
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D055515:Cysteine-Rich Protein 61 / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology
chemical_list: C501848:CCN1 protein, human; D055515:Cysteine-Rich Protein 61; C544266:MIRN100 microRNA, human; D035683:MicroRNAs