title: On the question of the identity of cytochrome b-560 in thylakoid stromal membranes.
abstract: Stromal membranes enriched in PS I contain a low potential cytochrome with a reduced α-band peak close to 560 nm. The identity of this cytochrome component has been ascribed either to a low potential form of the Photosystem II cytochrome b-559 or to a different cytochrome with a reduced α-band of 560 nm. The half-bandwidth of the 560 nm component in stromal membranes is identical to that of purified cytochrome b-559. Western blots show that the stromal membranes contain an amount of PS II cytochrome b-559 α-subunit that is more than sufficient to account for the cytochrome b-560 detected spectrophotometrically in these membranes. These immunochemical data and the similarity of (i) the spectral peaks, and (ii) the redox properties of low potential PS II cytochrome b-559 and the b-560 component, suggest that the simplest inference is that the cytochrome b-560 protein in stromal membranes is identical to the PS II cytochrome b-559.
authors: Department of Biological Sciences, Purdue University, 47907, West Lafayette, IN, USA.
authors: G S
authors: GS
authors: Tae
authors: R M
authors: RM
authors: Everly
authors: W A
authors: WA
authors: Cramer
authors: S A
authors: SA
authors: Madgwick
authors: P R
authors: PR
authors: Rich