title: SILAC peptide ratio calculator: a tool for SILAC quantitation of peptides and post-translational modifications.
abstract: This paper describes an algorithm to assist in relative quantitation of peptide post-translational modifications using stable isotope labeling by amino acids in cell culture (SILAC). The described algorithm first determines the normalization factor and then calculates SILAC ratios for a list of target peptide masses using precursor ion abundances. Four yeast histone mutants were used to demonstrate the effectiveness of this approach for quantitation of peptide post-translational modifications changes. The details of the algorithm's approach for normalization and peptide ratio calculation are described. The examples demonstrate the robustness of the approach as well as its utility to rapidly determine changes in peptide post-translational modifications within a protein.
authors: Department of Chemistry and Biochemistry, The Ohio State University , 100 West 18th Avenue, Columbus, Ohio 43210, United States.
authors: Xiaoyan
authors: X
authors: Guan
authors: Neha
authors: N
authors: Rastogi
authors: Mark R
authors: MR
authors: Parthun
authors: Michael A
authors: MA
authors: Freitas
mesh_terms: D000465:Algorithms; D007553:Isotope Labeling; D010455:Peptides / Q000032:analysis* / Q000378:metabolism; D011499:Protein Processing, Post-Translational
chemical_list: D010455:Peptides