title: Characterisation of the storage protein subunits synthesised in vitro by polyribosomes and RNA from developing pea (Pisum sativum L.) : I. Legumin.
abstract: Evidence is presented to show that legumin, the major storage protein in Pisum, is synthesised in vitro by the wheat germ and reticulocyte lysate systems, from polyribosomes and mRNA isolated from developing pea seeds. While legumin isolated from mature pea seeds consists of 40,000 and 20,000 MW subunits, the in vitro legumin is synthesised as a 60,000 MW precursor consisting of covalently linked 40,000 and 20,000 MW subunits. The implications of these findings are discussed in relationship to studies with other systems.
authors: Department of Botany, University of Durham, South Road, DH1 3LE, Durham, UK.
authors: R R
authors: RR
authors: Croy
authors: J A
authors: JA
authors: Gatehouse
authors: I M
authors: IM
authors: Evans
authors: D
authors: D
authors: Boulter