title: Characterization of the in vitro core surface proteome of Mycoplasma mycoides subsp. mycoides, the causative agent of contagious bovine pleuropneumonia.
abstract: Contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. mycoides (Mmm) is a severe cattle disease, present in many countries in sub-Saharan Africa. The development of improved diagnostic tests and vaccines for CBPP control remains a research priority. Polyacrylamide gel electrophoresis and mass spectrometry were used to characterize the Triton X-114 soluble proteome of nine Mmm strains isolated from Europe or Africa. Of a total of 250 proteins detected, 67 were present in all strains investigated. Of these, 44 were predicted to be lipoproteins or cytoplasmic membrane-associated proteins and are thus likely to be members of the core in vitro surface membrane-associated proteome of Mmm. Moreover, the presence of all identified proteins in other ruminant Mycoplasma pathogens were investigated. Two proteins of the core proteome were identified only in other cattle pathogens of the genus Mycoplasma pointing towards a role in host-pathogen interactions. The data generated will facilitate the identification and prioritization of candidate Mycoplasma antigens for improved control measures, as it is likely that surface-exposed membrane proteins will include those that are involved in host-pathogen interactions.
authors: Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale", via Campo Boario, 64100 Teramo, Italy. Electronic address: i.krasteva@izs.it.
authors: Ivanka
authors: I
authors: Krasteva
authors: International Livestock Research Institute, Old Naivasha Road, P.O. Box 30709, 00100 Nairobi, Kenya. Electronic address: a.liljander@cgiar.org.
authors: Anne
authors: A
authors: Liljander
authors: International Livestock Research Institute, Old Naivasha Road, P.O. Box 30709, 00100 Nairobi, Kenya; Molecular Biology and Bioinformatics Unit, International Centre for Insect Physiology and Ecology, Nairobi, Kenya. Electronic address: afischer@icipe.org.
authors: Anne
authors: A
authors: Fischer
authors: Moredun Proteomics Facility, Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK; Institute of Infection Immunity & Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, UK. Electronic address: david.g.e.smith@moredun.ac.uk.
authors: David G E
authors: DG
authors: Smith
authors: Moredun Proteomics Facility, Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK. Electronic address: neil.inglis@moredun.ac.uk.
authors: Neil F
authors: NF
authors: Inglis
authors: Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale", via Campo Boario, 64100 Teramo, Italy. Electronic address: m.scacchia@izs.it.
authors: Massimo
authors: M
authors: Scacchia
authors: Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale", via Campo Boario, 64100 Teramo, Italy. Electronic address: a.pini@izs.it.
authors: Attilio
authors: A
authors: Pini
authors: International Livestock Research Institute, Old Naivasha Road, P.O. Box 30709, 00100 Nairobi, Kenya. Electronic address: jores@web.de.
authors: Joerg
authors: J
authors: Jores
authors: Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale", via Campo Boario, 64100 Teramo, Italy. Electronic address: f.sacchini@izs.it.
authors: Flavio
authors: F
authors: Sacchini
mesh_terms: D000349:Africa; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002853:Chromatography, Liquid; D005060:Europe; D009174:Mycoplasma / Q000235:genetics / Q000378:metabolism; D009176:Mycoplasma mycoides / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D011002:Pleuropneumonia, Contagious / Q000175:diagnosis / Q000382:microbiology*; D020543:Proteome / Q000235:genetics*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D001425:Bacterial Outer Membrane Proteins; D020543:Proteome
keywords: CBPP; MS; Mass spectrometry; Membrane; Mmm; Mycoplasma mycoides subsp. mycoides; Surface proteome; Triton X-114; contagious bovine pleuropneumonia; mass spectrometry