title: Molecular profiling of the lateral habenula in a rat model of depression.
abstract: OBJECTIVE
  This study systematically investigated the effect of chronic mild stress and response to antidepressant treatment in the lateral habenula at the whole genome level.
  
  
  METHODS
  Rat whole genome expression chips (Affymetrix) were used to detect gene expression regulations in the lateral habenula of rats subjected to chronic mild stress (mild stressors exchanged twice a day for 8 weeks). Some rats received antidepressant treatment during fifth to eights week of CMS. The lateral habenula gene expression profile was studied through the gene ontology and signal pathway analyses using bioinformatics. Real-time quantitative polymerase chain reaction (RT-PCR) was used to verify the microarray results and determine the expression of the Fcrla, Eif3k, Sec3l1, Ubr5, Abca8a, Ankrd49, Cyp2j10, Frs3, Syn2, and Znf503 genes in the lateral habenula tissue.
  
  
  RESULTS
  In particular we found that stress and antidepressant treatment affected intracellular cascades like growth factor receptor signaling, G-protein-coupled receptor signaling, and Wnt signaling - processes involved in the neuroplastic changes observed during the progression of depression and antidepressant treatment.
  
  
  CONCLUSIONS
  The present study suggests an important role of the lateral habenula in the development of depression-like conditions and correlates to previous studies demonstrating a significant role of the lateral habenula in depressive-like conditions and antidepressant treatment.
authors: Translational Neuropsychiatry Unit, Department of Clinical Medicine, Aarhus University, Risskov, Denmark.
authors: Trine
authors: T
authors: Christensen
authors: Line
authors: L
authors: Jensen
authors: Elena V
authors: EV
authors: Bouzinova
authors: Ove
authors: O
authors: Wiborg
mesh_terms: D059445:Anhedonia / Q000187:drug effects; D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology* / Q000627:therapeutic use; D016000:Cluster Analysis; D003863:Depression / Q000150:complications / Q000188:drug therapy* / Q000235:genetics* / Q000523:psychology; D004195:Disease Models, Animal; D004435:Eating / Q000187:drug effects; D020869:Gene Expression Profiling; D019262:Habenula / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D013315:Stress, Psychological / Q000150:complications* / Q000235:genetics; D013395:Sucrose / Q000494:pharmacology
chemical_list: D000928:Antidepressive Agents; D013395:Sucrose