title: CE analysis of single wood cells performing hydrolysis and preconcentration in open microchannels.
abstract: In the present work, monosaccharides from pulp samples and single wood fibers were analyzed with CE, using indirect detection due to the lack of chromophores on the monosaccharides. The hydrolysis degradation of cellulose and hemicellulose into monosaccharides was performed using TFA, either in bulk scale or in microscale. In the microscale, one single wood fiber was hydrolyzed in an open microchannel manufactured on a silicon microchip with the dimensions 50 μm × 50 μm (length 1 or 3 cm). The low monosaccharide amounts derived from a single fiber implied that a preconcentration step was necessary to increase the detectability. Thus, an electromigration preconcentration of the hydrolyzed samples was performed within the microchannel, which resulted in a significantly enhanced signal intensity of the monosaccharides. In addition to the experimental study, computer simulations were performed regarding the preconcentration step of monosaccharides. The results from these simulations correlated well with the experimental results.
authors: Analytical Chemistry, Division of Applied Physical Chemistry, Department of Chemistry, School of Chemical Science and Engineering, KTH Royal Institute of Technology, Stockholm, Sweden.
authors: Maria Khihon
authors: MK
authors: Rokhas
authors: Saara
authors: S
authors: Mikkonen
authors: Juliane
authors: J
authors: Beyer
authors: Johan
authors: J
authors: Jacksén
authors: Åsa
authors: Å
authors: Emmer
mesh_terms: D003198:Computer Simulation; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006868:Hydrolysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D009005:Monosaccharides / Q000032:analysis* / Q000737:chemistry; D014934:Wood / Q000737:chemistry*
chemical_list: D009005:Monosaccharides
keywords: Capillary electrophoresis; Open microchannels; Preconcentration; Wood cells