title: [Expression of Gphaq in peripheral blood T lymphocytes of patients with polymyositis and its correlation with disease activity].
abstract: OBJECTIVE
  To analyze the expression of Gaq in peripheral blood T lymphocytes of the patients with polymyositis (PM) and its correlation with disease activity evaluated by the clinical markers [manual muscle test (MMT),myositis disease activity assessment (MDAA),creatine phosphokinase (CK), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP)].
  
  
  METHODS
  Blood samples were obtained from 30 patients with first onset PM and 30 healthy volunteers. Peripheral blood mononuclear cells (PBMCs) from these patients were collected and CD3+ T cells from PBMCs were sorted out with magnetic beans. The mRNA and protein expression levels of Galphaq in T cells were measured by RT-PCR and Western blot. Student's unpaired 2-tailed t-test was applied to identify Galphaq expression difference between PM patients and healthy controls. Pearson's correlation between Galphaq mRNA expression of PM and clinical evaluation markers MMT, MDAA, CK, ESR or CRP was determined.
  
  
  RESULTS
  Compared to health control, PM patients had significantly lower expression levels of Galphaq protein and mRNA in peripheral blood CD3+ T cells (P<0. 001). Negative correlation was found between Galphaq mRNA level and MMT (r= -0. 704, P<0. 001), MDAA (r= -0. 793, P<0. 001) or CK (r= -0. 670, P<0. 001), and no correlation was found between Galphaq mRNA level and ESR or CRP.
  
  
  CONCLUSIONS
  Galphaq expression in T cells of PM is lower than that in normal control and has negative correlation with PM disease activity.
authors: Rheumatology and Immunology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Min
authors: M
authors: Yang
authors: Yan
authors: Y
authors: Liang
authors: Yan
authors: Y
authors: He
authors: Chuan
authors: C
authors: Zuo
authors: Geng
authors: G
authors: Yin
authors: Qibing
authors: Q
authors: Xie
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017285:Polymyositis / Q000276:immunology* / Q000378:metabolism* / Q000503:physiopathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D043802:GTP-Binding Protein alpha Subunits, Gq-G11