title: Influence of different energy densities of laser phototherapy on oral wound healing.
abstract: The aim of the present prospective study was to evaluate the impact of laser phototherapy (LPT) on the healing of oral ulcers. Different power densities were used on oral wounds in Wistar rats (n=72) randomly divided into three groups: control (0 J/cm2), 4 J/cm2 laser, and 20 J/cm2 laser. Ulcers (3 mm in diameter) were made on the dorsum of the tongue with a punch. Irradiation with an indium-gallium-aluminum-phosphide laser (660 nm; output power: 40 mW; spot size: 0.04 cm2) was performed once a day in close contact with the ulcer for 14 consecutive days. A statistically significant acceleration in healing time was found with wounds treated with 4 J/cm2 LPT. Moreover, striking differences were found in the ulcer area, healing percentage, degree of reepithelialization, and collagen deposition. The most significant changes occurred after 5 days of irradiation. Based on the conditions employed in the present study, LPT is capable of accelerating the oral mucosa wound-healing process. Moreover, faster and more organized reepithelialization and tissue healing of the oral mucosa were achieved with an energy density of 4 J/cm2 in comparison to 20 J/cm2.
authors: Universidade Federal do Rio Grande do Sul, School of Dentistry, Department of Oral Pathology, Porto Alegre, Rio Grande do Sul, 90035-003, Brazil.
authors: Vivian Petersen
authors: VP
authors: Wagner
authors: Universidade Federal do Rio Grande do Sul, Hospital de Clínicas de Porto Alegre, Department of Pathology, Porto Alegre, Rio Grande do Sul, 90035-903, Brazil.
authors: Luise
authors: L
authors: Meurer
authors: Universidade Federal do Rio Grande do Sul, School of Dentistry, Department of Oral Pathology, Porto Alegre, Rio Grande do Sul, 90035-003, Brazil.
authors: Marco Antonio Trevizani
authors: MA
authors: Martins
authors: Universidade Federal do Rio Grande do Sul, School of Dentistry, Department of Oral Pathology, Porto Alegre, Rio Grande do Sul, 90035-003, Brazil.
authors: Chris Krebs
authors: CK
authors: Danilevicz
authors: Universidade Federal do Rio Grande do Sul, School of Dentistry, Department of Oral Pathology, Porto Alegre, Rio Grande do Sul, 90035-003, Brazil.
authors: Alessandra Selinger
authors: AS
authors: Magnusson
authors: Universidade de São Paulo, School of Dentistry, Department of Dentistry, São Paulo, 05508-000 Brazil.
authors: Márcia Martins
authors: MM
authors: Marques
authors: Universidade Federal do Rio Grande do Sul, School of Dentistry, Department of Oral Pathology, Porto Alegre, Rio Grande do Sul, 90035-003, Brazil.
authors: Manoel Sant'Ana
authors: MS
authors: Filho
authors: University of Michigan School of Dentistry, Laboratory of Epithelial Biology, Department of Periodontics and Oral Medicine, Ann Arbor, Michigan 48109-1078.
authors: Cristiane Helena
authors: CH
authors: Squarize
authors: Universidade Federal do Rio Grande do Sul, School of Dentistry, Department of Oral Pathology, Porto Alegre, Rio Grande do Sul, 90035-003, Brazil.
authors: Manoela Domingues
authors: MD
authors: Martins
mesh_terms: D000818:Animals; D007249:Inflammation / Q000473:pathology; D054023:Lasers, Semiconductor; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D009061:Mouth Mucosa / Q000473:pathology / Q000528:radiation effects; D019226:Oral Ulcer / Q000473:pathology / Q000628:therapy*; D011446:Prospective Studies; D051381:Rats; D017208:Rats, Wistar; D062326:Re-Epithelialization / Q000528:radiation effects*