title: miR-124 represses ROCK1 expression to promote neurite elongation through activation of the PI3K/Akt signal pathway.
abstract: Recent studies have demonstrated an important role for miR-124, the most abundant and well-conserved brain-specific microRNA(miRNA), in promoting neurite outgrowth and elongation during neuronal differentiation. This miRNA's target genes and the mechanisms that execute this role remain unclear. In this study, we identified ROCK1, a small GTPase Rho kinase, as a direct target of miR-124 for regulating neurite elongation. miR-124 significantly inhibited ROCK1 expression in M17 cells. Inhibiting ROCK1 promoted neurite elongation, and the overexpression of ROCK1 strongly repressed the neurite elongation-enhancing effect of miR-124 in M17 cells. We determined that Akt functions as a novel ROCK1 downstream effector in regulating neurite outgrowth and elongation.
authors: Department of Neurobiology, Southern Medical University, Guangzhou, Guangdong Province, China.
authors: Xi
authors: X
authors: Gu
authors: Siying
authors: S
authors: Meng
authors: Shuhu
authors: S
authors: Liu
authors: Chunhong
authors: C
authors: Jia
authors: Yingying
authors: Y
authors: Fang
authors: Shuji
authors: S
authors: Li
authors: Congcong
authors: C
authors: Fu
authors: Qiancheng
authors: Q
authors: Song
authors: Lifang
authors: L
authors: Lin
authors: Xuemin
authors: X
authors: Wang
mesh_terms: D000818:Animals; D048708:Cell Growth Processes; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D057809:HEK293 Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D016501:Neurites / Q000378:metabolism* / Q000502:physiology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction; D054460:rho-Associated Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: C548144:MIRN124 microRNA, rat; D035683:MicroRNAs; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D054460:rho-Associated Kinases