title: Semaphorin 7A is expressed on airway eosinophils and upregulated by IL-5 family cytokines.
abstract: Semaphorin 7A (sema7a) plays a major role in TGF-β1-induced lung fibrosis. Based on the accumulating evidence that eosinophils contribute to fibrosis/remodeling in the airway, we hypothesized that airway eosinophils may be a significant source of sema7a. In vivo, sema7a was expressed on the surface of circulating eosinophils and upregulated on bronchoalveolar lavage eosinophils obtained after segmental bronchoprovocation with allergen. Based on mRNA levels in unfractionated and isolated bronchoalveolar cells, eosinophils are the predominant source of sema7a. In vitro, among the members of the IL-5-family cytokines, sema7a protein on the surface of blood eosinophils was increased more by IL-3 than by GM-CSF or IL-5. Cytokine-induced expression of cell surface sema7a required translation of newly synthesized protein. Finally, a recombinant sema7a induced alpha-smooth muscle actin production in human bronchial fibroblasts. semaphorin 7A is a potentially important modulator of eosinophil profibrotic functions in the airway remodeling of patients with chronic asthma.
authors: Department of Medicine, Allergy, Pulmonary, and Critical Care Medicine Division, University of Wisconsin School of Medicine and Public Health, Madison, WI 53792, USA. Electronic address: sesnault@medicine.wisc.edu.
authors: Stephane
authors: S
authors: Esnault
authors: Department of Medicine, Allergy, Pulmonary, and Critical Care Medicine Division, University of Wisconsin School of Medicine and Public Health, Madison, WI 53792, USA.
authors: Elizabeth A
authors: EA
authors: Kelly
authors: Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, WI 53792, USA.
authors: Mats W
authors: MW
authors: Johansson
authors: Department of Medicine, Allergy, Pulmonary, and Critical Care Medicine Division, University of Wisconsin School of Medicine and Public Health, Madison, WI 53792, USA.
authors: Lin Ying
authors: LY
authors: Liu
authors: Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, WI 53792, USA.
authors: Shih-Tsung
authors: ST
authors: Han
authors: Department of Medicine, Allergy, Pulmonary, and Critical Care Medicine Division, University of Wisconsin School of Medicine and Public Health, Madison, WI 53792, USA.
authors: Moneeb
authors: M
authors: Akhtar
authors: Department of Medicine, Allergy, Pulmonary, and Critical Care Medicine Division, University of Wisconsin School of Medicine and Public Health, Madison, WI 53792, USA.
authors: Nathan
authors: N
authors: Sandbo
authors: Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, WI 53792, USA.
authors: Deane F
authors: DF
authors: Mosher
authors: Department of Medicine, Allergy, Pulmonary, and Critical Care Medicine Division, University of Wisconsin School of Medicine and Public Health, Madison, WI 53792, USA.
authors: Loren C
authors: LC
authors: Denlinger
authors: Department of Medicine, Allergy, Pulmonary, and Critical Care Medicine Division, University of Wisconsin School of Medicine and Public Health, Madison, WI 53792, USA.
authors: Sameer K
authors: SK
authors: Mathur
authors: Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
authors: James S
authors: JS
authors: Malter
authors: Department of Medicine, Allergy, Pulmonary, and Critical Care Medicine Division, University of Wisconsin School of Medicine and Public Health, Madison, WI 53792, USA.
authors: Nizar N
authors: NN
authors: Jarjour
mesh_terms: D000199:Actins / Q000276:immunology; D000485:Allergens / Q000008:administration & dosage; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D004804:Eosinophils / Q000276:immunology*; D005347:Fibroblasts; D058851:GPI-Linked Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000097:blood / Q000276:immunology; D015848:Interleukin-5 / Q000276:immunology*; D008168:Lung / Q000166:cytology / Q000276:immunology*; D039961:Semaphorins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D015854:Up-Regulation
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D000485:Allergens; D015703:Antigens, CD; D058851:GPI-Linked Proteins; C508599:IL5 protein, human; D015848:Interleukin-5; C118753:SEMA7A protein, human; D039961:Semaphorins
keywords: BAL; EOS; Eosinophil; Fibrosis; IL-3; RT-qPCR; SBP-Ag; Sema7a; Semaphorin 7A; Translation; bronchoalveolar lavage; eosinophils; real-time quantitative PCR; segmental bronchoprovocation with allergen; semaphorin 7A.