title: [Is resistance to disinfectants a mandatory feature of a hospital strain of causative agents of nosocomial purulent-septic infections?].
abstract: OBJECTIVE
  Evaluate resistance to working solutions of disinfectants by Staphylococcus haemolyticus and Klebsiella pneumoniae isolated from newborns and hospital environment objects of obstetric hospital during registration of group purulent-septic infections (PSI).
  
  
  METHODS
  Analysis of 2 epidemic situations on PSI morbidity of newborns caused by S. haemolyticus and K. pneumoniae was carried out. Sensitivity to antibiotics of S. haemolyticus and K. pneumoniae strains isolated from newborns and hospital environment was studied by disc-diffusion method and genotyping of K. pneumoniae--by using polymerase chain reaction with universal primer M 13 (RAPD-PCR). Sensitivity of S. haemolyticus and K. pneumoniae to working solutions of disinfectants was determined on test-surfaces (glass, metal, plastic, wood, oilcloth).
  
  
  RESULTS
  The detected identity of antibiotic phenotype of S. haemolyticus and K. pneumoniae strains as well as genotype of K. pneumoniae strains combined with registration of group PSI morbidity among newborns confirms that the circulating strains (clones) of the causative agents were hospital. S. haemolyticus and K. pneumoniae strains in most cases were sensitive to working solutions of disinfectants.
  
  
  CONCLUSIONS
  Resistance of causative agents of nosocomial PSI to disinfectants is not an unconditional feature of a hospital strain, and concurrence of resistance profile of microorganisms to disinfectant preparations--a mandatory feature of the presence of epidemiologic connection between the diseased.
authors: V I
authors: VI
authors: Sergevnin
authors: N G
authors: NG
authors: Zueva
authors: T V
authors: TV
authors: Kliukina
authors: E O
authors: EO
authors: Volkova
mesh_terms: D003428:Cross Infection / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D004202:Disinfectants / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D006801:Humans; D007231:Infant, Newborn; D007710:Klebsiella Infections / Q000382:microbiology / Q000517:prevention & control*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics / Q000472:pathogenicity; D008826:Microbial Sensitivity Tests; D041162:Staphylococcus haemolyticus / Q000187:drug effects / Q000235:genetics / Q000472:pathogenicity
chemical_list: D004202:Disinfectants