title: Crystallization of the C-terminal domain of the fibre protein from snake adenovirus 1, an atadenovirus.
abstract: Adenovirus fibre proteins play an important role in determining viral tropism. The C-terminal domain of the fibre protein from snake adenovirus type 1, a member of the Atadenovirus genus, has been expressed, purified and crystallized. Crystals were obtained belonging to space groups P2(1)2(1)2(1) (two different forms), I2(1)3 and F23. The best of these diffracted synchrotron radiation to a resolution of 1.4 Å. As the protein lacks methionines or cysteines, site-directed mutagenesis was performed to change two leucine residues to methionines. Crystals of selenomethionine-derivatized crystals of the I2(1)3 form were also obtained and a multi-wavelength anomalous dispersion data set was collected.
authors: Departamento de Estructura de Macromoléculas, Centro Nacional de Biotecnología (CNB-CSIC), Calle Darwin 3, 28049 Madrid, Spain.
authors: Abhimanyu K
authors: AK
authors: Singh
authors: Rosa
authors: R
authors: Menéndez-Conejero
authors: Carmen
authors: C
authors: San Martín
authors: Mark J
authors: MJ
authors: van Raaij
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D046808:Atadenovirus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D036022:Capsid Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D007076:Iguanas / Q000821:virology; D007930:Leucine / Q000737:chemistry* / Q000235:genetics; D008715:Methionine / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D032383:Myocytes, Cardiac / Q000166:cytology / Q000821:virology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012645:Selenomethionine / Q000737:chemistry / Q000378:metabolism; D012911:Snakes / Q000821:virology; D017356:Synchrotrons
chemical_list: D036022:Capsid Proteins; D011994:Recombinant Proteins; C018315:hexon capsid protein, Adenovirus; D012645:Selenomethionine; D008715:Methionine; D007930:Leucine
keywords: snake adenovirus 1 fibre protein