title: Huaier polysaccharides suppresses hepatocarcinoma MHCC97-H cell metastasis via inactivation of EMT and AEG-1 pathway.
abstract: We have recently reported that astrocyte elevated gene-1 (AEG-1) might be an epithelial-mesenchymal transition (EMT) associated biomarker in human hepatocellular carcinoma (HCC), and play an important role in the progression of hepatocellular carcinoma. To extend our study, we examined here the anti-invasive and metastatic effects of Huaier polysaccharide (HP) on human HCC cell line MHCC97-H and explored its possible mechanism of action. Treatment with HP dose-dependently inhibited the proliferation, adhesion, migration and invasion of MHCC97-H cells in vitro. This was achieved not only by reducing the expression of AEG-1 and N-cadherin, but also by enhancing E-cadherin expression. Therefore, these data suggested that HP can inhibit the growth and metastatic potential of MHCC97-H cells through modulation of the AEG-1/EMT pathway.
authors: Intervention Therapy Center of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing 100069, China; Beijing Institute of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing 100069, China. Electronic address: jiashengzheng@outlook.com.
authors: Jiasheng
authors: J
authors: Zheng
authors: Intervention Therapy Center of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing 100069, China; Beijing Institute of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing 100069, China.
authors: Cong
authors: C
authors: Li
authors: Department of Infectious Disease, the Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China.
authors: Xia
authors: X
authors: Wu
authors: Department of Pharmacology, College of Pharmacy, Harbin Medical University, Harbin 150081, China.
authors: Mengmeng
authors: M
authors: Liu
authors: School of Medicine, Tsinghua Center for Life Sciences, Tsinghua University, Beijing 100084, China.
authors: Xicai
authors: X
authors: Sun
authors: Department of Infectious Disease, the Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China.
authors: Yingzhuo
authors: Y
authors: Yang
authors: Intervention Therapy Center of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing 100069, China; Beijing Institute of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing 100069, China.
authors: Meijun
authors: M
authors: Hao
authors: Intervention Therapy Center of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing 100069, China; Beijing Institute of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing 100069, China.
authors: Shoupeng
authors: S
authors: Sheng
authors: Intervention Therapy Center of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing 100069, China; Beijing Institute of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing 100069, China.
authors: Yu
authors: Y
authors: Sun
authors: Intervention Therapy Center of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing 100069, China; Beijing Institute of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing 100069, China.
authors: Honghai
authors: H
authors: Zhang
authors: Intervention Therapy Center of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing 100069, China; Beijing Institute of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing 100069, China.
authors: Jiang
authors: J
authors: Long
authors: Department of Nutrition and Food Hygiene, Public Health College of Guiyang Medical Colleague, Guiyang 550000, China; School of Public Health, Shenyang Medical College, Shenyang 110034, China.
authors: Yi
authors: Y
authors: Liang
authors: Intervention Therapy Center of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing 100069, China; Beijing Institute of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing 100069, China.
authors: Caixia
authors: C
authors: Hu
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D015820:Cadherins / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology*; D008565:Membrane Proteins; D009362:Neoplasm Metastasis; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D016601:RNA-Binding Proteins; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D000970:Antineoplastic Agents; D015820:Cadherins; D015815:Cell Adhesion Molecules; C483039:MTDH protein, human; D008565:Membrane Proteins; D010936:Plant Extracts; D011134:Polysaccharides; D016601:RNA-Binding Proteins
keywords: Anti-metastasis; Astrocyte elevated gene-1 (AEG-1); Hepatocellular carcinoma; Huaier polysaccharide; MHCC97-H cells