title: Gd3+-DTPA-bis (N-methylamine) - anionic linear globular Dendrimer-G1; a more efficient MRI contrast media.
abstract: By advancing of molecular imaging techniques, magnetic resonance imaging (MRI) is becoming an increasingly important tool in early diagnosis. Researchers have found new ways to increase contrast of MRI images.Therefore some types of drug known as contrast media are produced. Contrast media improve the visibility of internal body structures in MRI images. Gadodiamide (Omniscan®) is one of these contrast media which is produced commercially and used clinically. In this study Gadodiamide was first synthesized and then qualitative and quantitative methods were carried out to ensure the proper synthesis of this drug then to increase the efficiency of this contrast medium use dendrimer that is one kind of nano particle. This dendrimer has a polyethylene glycol (PEG) core and citric acid branches. After dendrimer attached to Gadodiamide to ensure the proper efficient connection between them the stability studies were carried out and cytotoxicity of the drug was evaluated. Finally, after ensuring the non-toxicity of the drug, in vivo studies (injected into mice) MR imaging was performed to examine the impact of synthesis drug on the resolution of image.The result obtained from this study demonstrated that the attachment of Gadodiamide to dendrimer reduces its cytotoxicity and also improved resolution of image. Also the new contrast media (Gd3+-DTPA- bis [N-methylamine] - Dendrimer) - unlike Omniscan® - is biodegradable and able to enter the HEPG2 cell line. The results confirm the hypothesis that using dendrimer to synthesize this new nano contrast medium increases its effectiveness.
authors: Department of Biology, Faculty of Sciences, Science and Research Campus, Islamic Azad University, Tehran, Iran.
authors: N
authors: N
authors: Ghalandarlaki
authors: Department of Biology, Faculty of Sciences, Science and Research Campus, Islamic Azad University, Tehran, Iran.
authors: T D
authors: TD
authors: Mohammadi
authors: Cellular and Molecular Department, School of Medicine, Islamic Azad -University, Tehran, Iran.
authors: R
authors: R
authors: Agha Babaei
authors: Department of Biology, Faculty of Sciences, Science and Research Campus, Islamic Azad University, Tehran, Iran.
authors: M A
authors: MA
authors: Tabasi
authors: School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors: P
authors: P
authors: Keyhanvar
authors: Department of Nanomedicine, Shahid Beheshti Medical University, Tehran, Iran.
authors: B
authors: B
authors: Mehravi
authors: Department of Biology, Faculty of Sciences, Science and Research Campus, Islamic Azad University, Tehran, Iran.
authors: P
authors: P
authors: Yaghmaei
authors: Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
authors: R A
authors: RA
authors: Cohan
authors: Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors: M S
authors: MS
authors: Ardestani
mesh_terms: D003287:Contrast Media; D050091:Dendrimers / Q000138:chemical synthesis / Q000737:chemistry; D019786:Gadolinium DTPA / Q000138:chemical synthesis / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*
chemical_list: D003287:Contrast Media; D050091:Dendrimers; C064925:gadodiamide; D019786:Gadolinium DTPA