title: Diversity and saline resistance of endophytic fungi associated with Pinus thunbergii in coastal shelterbelts of Korea.
abstract: The Black Pine, Pinus thunbergii, is widely distributed along the eastern coast of Korea and its importance as a shelterbelt was highlighted after tsunamis in Indonesia and Japan. The root endophytic diversity of P. thunbergii was investigated in three coastal regions; Goseong, Uljin, and Busan. Fungi were isolated from the root tips, and growth rates of pure cultures were measured and compared between PDA with and without 3% NaCl to determine their saline resistance. A total of 259 isolates were divided into 136 morphotypes, of which internal transcribed spacer region sequences identified 58 species. Representatives of each major fungi phylum were present: 44 Ascomycota, 8 Zygomycota, and 6 Basidiomycota. Eighteen species exhibited saline resistance, many of which were Penicillium and Trichoderma species. Shoreline habitats harbored higher saline-tolerant endophytic diversity compared with inland sites. This investigation indicates that endophytes of P. thunbergii living closer to the coast may have higher resistance to salinity and potentially have specific relationships with P. thunbergii.
authors: School of Biological Sciences, Seoul National University, Seoul 151-747, Republic of Korea.
authors: Young Ju
authors: YJ
authors: Min
authors: Myung Soo
authors: MS
authors: Park
authors: Jonathan J
authors: JJ
authors: Fong
authors: Ying
authors: Y
authors: Quan
authors: Sungcheol
authors: S
authors: Jung
authors: Young Woon
authors: YW
authors: Lim
mesh_terms: D044822:Biodiversity; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D060026:Endophytes / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D005658:Fungi / Q000145:classification* / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D007723:Korea; D008969:Molecular Sequence Data; D009997:Osmotic Pressure; D010802:Phylogeny; D028223:Pinus / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology; D012462:Saline Solution, Hypertonic / Q000378:metabolism*; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012462:Saline Solution, Hypertonic