title: Assessment of anti-inflammatory effect of 5β-hydroxypalisadin B isolated from red seaweed Laurencia snackeyi in zebrafish embryo in vivo model.
abstract: 5β-Hydroxypalisadin B, a halogenated secondary metabolite isolated from red seaweed Laurencia snackeyi was evaluated for its anti-inflammatory activity in lipopolysaccharide (LPS)-induced zebrafish embryo. Preliminary studies suggested the effective concentrations of the compound as 0.25, 0.5, 1 μg/mL for further in vivo experiments. 5β-Hydroxypalisadin B, exhibited profound protective effect in the zebrafish embryo as confirmed by survival rate, heart beat rate, and yolk sac edema size. The compound acts as an effective agent against reactive oxygen species (ROS) formation induced by LPS and tail cut. Moreover, 5β-hydroxypalisadin B effectively inhibited the LPS-induced nitric oxide (NO) production in zebrafish embryo. All the tested protective effects of 5β-hydroxypalisadin B were comparable to the well-known anti-inflammatory agent dexamethasone. According to the results obtained, 5β-hydroxypalisadin B isolated from red seaweed L. snackeyi could be considered as an effective anti-inflammatory agent which might be further developed as a functional ingredient.
authors: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors: W A J P
authors: WA
authors: Wijesinghe
authors: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Eun-A
authors: EA
authors: Kim
authors: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Min-Cheol
authors: MC
authors: Kang
authors: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Won-Woo
authors: WW
authors: Lee
authors: Marine Natural Products Laboratory, Korea Ocean Research & Development Institute, P.O. Box 29, Ansan 426-744, Republic of Korea.
authors: Hyi-Seung
authors: HS
authors: Lee
authors: Laboratory of Natural Products Chemistry, Institute for Tropical Biology and Conservation, University Malaysia Sabah, Kota Kinabalu 88440, Sabah, Malaysia. Electronic address: csv@ums.edu.my.
authors: Charles S
authors: CS
authors: Vairappan
authors: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: youjinj@jejunu.ac.kr.
authors: You-Jin
authors: YJ
authors: Jeon
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism; D044722:Laurencia / Q000737:chemistry; D008070:Lipopolysaccharides; D009569:Nitric Oxide / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D015027:Zebrafish
chemical_list: D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D012717:Sesquiterpenes; C505296:palisadin B; D009569:Nitric Oxide
keywords: 5β-hydroxypalisadin B; Anti-inflammatory; Functional ingredient; Laurencia snackeyi; Zebrafish embryo