title: Antihyperglycaemic and antioxidant effects of Adenia lobata Engl. (Passifloraceae) in streptozotocin-induced diabetic rats.
abstract: The antihyperglycaemic and antioxidant activities of a Ghanaian medicinal plant namely Adenia lobata Engl (Passifloraceae), used to treat diabetes mellitus in traditional medicine, was investigated. The dried stem powder of A. lobata was successively extracted by Soxhlet with petroleum ether and 70% ethanol to obtain the crude petroleum ether (PEAL: yield =1.1 w/w %) and ethanol (EEAL: yield = 5.4 w/w %) extracts. The extracts were assessed for their antihyperglycaemic and antioxidant activities. The antihyperglycaemic activity of PEAL and EEAL were determined in streptozotocin-induced diabetic rats (70 mg/kg body weight). Five groups of diabetic rats were given 150, 300 and 600 mg/kg body weight of PEAL and EEAL orally once daily for 20 days. Glibenclamide (5 mg/kg body weight) was used as positive control while distilled water (5 ml) acted as the normal diabetic control. The blood glucose levels were monitored initially for 6 hours and subsequently over 24 days. Both extracts exhibited statistically significant (p< 0.001) antihyperglycaemic activity throughout the study period, with EEAL showing the greatest activity. The antioxidant properties of the petroleum ether and ethanol extracts of A. lobata (PEAL and EEAL) were evaluated using five assays; total phenolic content, total antioxidant capacity, reducing power, DPPH scavenging effect and lipid peroxidation activity. In all these assays, the antioxidant properties increased with increasing concentration of the extracts.
authors: Department of Phytochemistry, Centre for Scientific Research into Plant Medicine, Mampong-Akwapim, Ghana.
authors: Sarkodie Adusei
authors: SA
authors: Joseph
authors: Fleischer Christian
authors: FC
authors: Theophilus
authors: Edoh Adotei
authors: EA
authors: Dominic
authors: Dickson Akosua
authors: DA
authors: Rita
authors: Mensah Lincoln
authors: ML
authors: Kwaw
authors: Annan
authors: A
authors: Kofi
authors: Woode
authors: W
authors: Eric
authors: Koffour Asumeng
authors: KA
authors: George
authors: Appiah Ampomah
authors: AA
authors: Alfred
authors: Brew-Daniels
authors: BD
authors: Henry
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D031683:Passifloraceae / Q000737:chemistry*; D010636:Phenols / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018547:Plant Stems; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D010636:Phenols; D010936:Plant Extracts
keywords: Adenia lobata; Antihyperglycaemic; antioxidant; diabetes