title: [Suppressive effects of gamma-secretase inhibitor DAPT on the proliferation of pancreatic cancer cells].
abstract: OBJECTIVE
  To explore the effect of DAPT, a gamma-secretase inhibitor on the proliferation of pancreatic cancer cells and its underlying mechanism.
  
  
  METHODS
  Three human pancreatic cancer cell lines as AsPC-1, BxPC-3 and MIAPaCa-2 were employed for in vitro experiments. Cell proliferation was tested by CCK-8 and cell apoptosis was examined by Annexin V/PI staining after the treatment of various concentrations of DAPT at different time.
  
  
  RESULTS
  DAPT of 100 micromol/L significantly inhibited the proliferation of these 3 cell lines from day 3 to 5. DAPT of 50 micromol/L inhibited the proliferation of BxPC-3 and MIAPaCa-2 cells from day 3 to 5, whereas the effect of inhibition was only observed at day 5 in AsPC-I cell. DAPT of 10 micromol/L inhibited the proliferation of BxPC-3 and MIAPaCa-2 cells at day 5, whereas no effect was found in AsPC-1. Flow cytometry test showed that cell apoptosis rate was higher in DAPT treatment groups compared with control group.
  
  
  CONCLUSIONS
  DAPT could suppress the proliferation of pancreatic cancer cell, with the mechanism of promoting cell apoptosis.
authors: West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Xiao
authors: X
authors: Du
authors: Zhong
authors: Z
authors: Cheng
authors: Yang
authors: Y
authors: Li
authors: Zong-Guang
authors: ZG
authors: Zhou
authors: Lie
authors: L
authors: Yang
authors: Ming-Ming
authors: MM
authors: Zhang
mesh_terms: D053829:Amyloid Precursor Protein Secretases / Q000037:antagonists & inhibitors*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004151:Dipeptides / Q000494:pharmacology*; D006801:Humans; D010190:Pancreatic Neoplasms / Q000473:pathology*; D051880:Receptors, Notch / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D004151:Dipeptides; C419410:N-(N-(3,5-difluorophenacetyl)alanyl)phenylglycine tert-butyl ester; D051880:Receptors, Notch; D053829:Amyloid Precursor Protein Secretases