title: Clinical application of cultured epithelial autografts on acellular dermal matrices in the treatment of extended burn injuries.
abstract: Achieving permanent replacement of skin in extensive full-thickness and deep partial-thickness burn injuries and chronic wounds remains one of the fundamental surgical problems. Presently, split-thickness skin grafts are still considered the best material for surgical repair of an excised burn wound. However, in burns that affect greater than 50% of total body surface area, the patient has insufficient areas of unaffected skin from which split-thickness skin grafts can be harvested. The use of cultured epithelial (or epidermal) autografts (CEAs) has achieved satisfactory results. But the take rate of CEAs is poor in full-thickness bed or in chronically infected area. Providing temporary cover with allograft skin, or a more permanent allodermis, may increase clinical take. This review aims to (1) describe the use of CEAs in the regeneration of the epidermis, (2) introduce the application of the acellular dermal matrices (ADMs) in the clinics, and (3) enhance understanding of the CEAs applied with ADM as an appropriate strategy to treat the extended burn injuries. The current evidence regarding the cultured epithelial cell or keratinocyte autograft and dermal grafts applied in the treatment of burn injuries was investigated with an extensive electronic and manual search (MEDLINE and EMBASE). The included literature (N=136 publications) was critically evaluated focusing on the efficacy and safety of this technique in improving the healing of the deep dermal and full-thickness burn injuries. This review concluded that the use of ADM with CEAs is becoming increasingly routine, particularly as a life-saving tool after acute thermal trauma.
authors: From the *Department of Orthopaedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China; †Division of Plastic Surgery, University of Mississippi Medical Center, Jackson; and ‡Rakin Medical Center, Brandon, MS.
authors: Taolin
authors: T
authors: Fang
authors: William C
authors: WC
authors: Lineaweaver
authors: Frederick C
authors: FC
authors: Sailes
authors: Carson
authors: C
authors: Kisner
authors: Feng
authors: F
authors: Zhang
mesh_terms: D062088:Acellular Dermis; D064592:Autografts / Q000637:transplantation*; D002056:Burns / Q000601:surgery*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000637:transplantation*; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D015603:Keratinocytes / Q000637:transplantation; D016038:Skin Transplantation / Q000379:methods*; D014182:Transplantation, Autologous / Q000379:methods