title: Activation of cathepsins B and L in mouse lymphosarcoma tissue under the effect of cyclophosphamide is associated with apoptosis induction and infiltration by mononuclear phagocytes.
abstract: We analyzed activities of lysosomal cystein cathepsins B and L in mouse LS lymphosarcoma and its drug-resistant RLS 40 strain and their correlations with the dynamics of the percentage of cells with fragmented DNA and CD14 (+) phagocytes over 3 days after cyclophosphamide injection. LS regression and inhibition of RLS 40 growth after cyclophosphamide injection were paralleled by an increase in cathepsins B and L activities in tumor tissues. The antitumor effect of cyclophosphamide associated with apoptosis intensity and protease activities were significantly higher in LS. Positive correlations between activities of cathepsins B and L and the LS tissue content of cells with fragmented DNA and CD14 (+) phagocytes and negative correlations thereof with tumor weight were detected. It seems that the increase in cathepsins B and L activities in LS tissues was caused by cyclophosphamide induction of apoptosis and depended on the level of tumor cell infiltration with mononuclear phagocytes.
authors: Research Institute of Physiology, Siberian Division of the Russian Academy of Medical Sciences; Research Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia. s.zhanaeva@physiol.ru.
authors: S Ya
authors: SY
authors: Zhanaeva
authors: E V
authors: EV
authors: Mel'nikova
authors: V A
authors: VA
authors: Trufakin
authors: T A
authors: TA
authors: Korolenko
mesh_terms: D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002401:Cathepsin B / Q000378:metabolism*; D056668:Cathepsin L / Q000378:metabolism*; D003520:Cyclophosphamide / Q000494:pharmacology*; D053938:DNA Fragmentation; D004789:Enzyme Activation; D008228:Lymphoma, Non-Hodgkin / Q000188:drug therapy / Q000201:enzymology* / Q000276:immunology / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009368:Neoplasm Transplantation; D010586:Phagocytes / Q000187:drug effects / Q000276:immunology*
chemical_list: D018906:Antineoplastic Agents, Alkylating; D003520:Cyclophosphamide; D002401:Cathepsin B; C533915:Ctsb protein, mouse; D056668:Cathepsin L; C534278:Ctsl protein, mouse