title: Temporal-spatial expression of ENOLASE after acute spinal cord injury in adult rats.
abstract: ENOLASE enzymes are abundantly expressed, cytosolic carbon-oxygen lyases known for their role in glucose metabolism. Recent accumulation of evidence revealed that, in addition to its glycolytic function, enolase is also associated with ischemia, hypoxia and to be a neurotrophic factor. To analysis the certain expression and biological function in central nervous system, we performed an acute spinal cord contusion injury model in adult rats. Western blot analysis indicated a marked upregulation of ENOLASE after spinal cord injury (SCI). Immunohistochemistry revealed wide distribution of enolase in spinal cord, including neurons and glial cells. Double immunofluorescent staining for proliferating cell nuclear antigen and phenotype-specific markers showed increases of enolase expression in proliferating microglia and astrocytes. Our data suggest that enolase may be implicated in the proliferation of microglia and astrocytes after SCI.
authors: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, People's Republic of China.
authors: Ming
authors: M
authors: Li
authors: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, People's Republic of China.
authors: Hai
authors: H
authors: Wen
authors: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, People's Republic of China.
authors: Zhiguang
authors: Z
authors: Yan
authors: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, People's Republic of China.
authors: Tao
authors: T
authors: Ding
authors: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, People's Republic of China.
authors: Long
authors: L
authors: Long
authors: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, People's Republic of China.
authors: Hongjiu
authors: H
authors: Qin
authors: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, People's Republic of China.
authors: Hua
authors: H
authors: Wang
authors: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, People's Republic of China. Electronic address: zhangfengdoctor@sina.com.
authors: Feng
authors: F
authors: Zhang
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D008297:Male; D010751:Phosphopyruvate Hydratase / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000201:enzymology*; D013119:Spinal Cord Injuries / Q000201:enzymology*
chemical_list: D010751:Phosphopyruvate Hydratase
keywords: ENOLASE; Glial proliferation; Rats; Spinal cord injury