title: Whole blood cells loaded with messenger RNA as an anti-tumor vaccine.
abstract: The use of a cell-based vaccine composed of autologous whole blood cells loaded with mRNA is described. Mice immunized with whole blood cells loaded with mRNA encoding antigen develop anti-tumor immunity comparable to DC-RNA immunization. This approach offers a simple and affordable alternative to RNA-based cellular therapy by circumventing complex, laborious and expensive ex vivo manipulations required for DC-based immunizations.
authors: Department of Chemical & Biomolecular Engineering, National University of Singapore, Singapore, 117576; Department of Biomedical Engineering, Duke University, Durham, NC, 27708, USA.
authors: Kyle K L
authors: KK
authors: Phua
authors: David
authors: D
authors: Boczkowski
authors: Jens
authors: J
authors: Dannull
authors: Scott
authors: S
authors: Pruitt
authors: Kam W
authors: KW
authors: Leong
authors: Smita K
authors: SK
authors: Nair
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D001773:Blood Cells / Q000166:cytology / Q000378:metabolism* / Q000637:transplantation; D019496:Cancer Vaccines / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D004195:Disease Models, Animal; D018274:Electroporation; D005260:Female; D007167:Immunotherapy; D019746:Intramolecular Oxidoreductases / Q000235:genetics / Q000378:metabolism; D008546:Melanoma, Experimental / Q000401:mortality / Q000473:pathology / Q000628:therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D015996:Survival Rate
chemical_list: D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D012333:RNA, Messenger; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase
keywords: blood; cancer; immunotherapy; messenger RNA; vaccine