title: Genotoxicity and reduced heat shock protein 70 in human airway smooth muscle cells exposed to cigarette smoke extract.
abstract: Cigarette smoke is associated with the development of several diseases, such as chronic obstructive pulmonary disease (COPD). The purpose of this study was to investigate genotoxicity and heat shock protein 70 (Hsp70) in human airway smooth muscle cells (HASMCs) exposed to cigarette smoke extract (CSE). HASMCs was exposed to CSE with different doses for 24 h. The level of 8-hydroxydeoxyguanosine (8-OHdG) was determined by using HPLC-ECD, the DNA damage was analyzed by using comet assay, and apoptosis was examined by using Annexin-FITC/PI staining. The production of Hsp70 after CSE stimulation was tested. Results indicated that CSE significantly increased the level of 8-OHdG, DNA damage and cell apoptosis, and reduced the production of Hsp70. In particular, levels of Hsp70 were inversely correlated with 8-OHdG, DNA damage and cell apoptosis. It was concluded that cigarette smoke induced genotoxicity and decreased the production of cell protective protein Hsp70, which may contribute to the development of some airway diseases.
authors: Department of Respiratory Medicine and Critical Care Medicine, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors: Xiao-Jie
authors: XJ
authors: Wu
authors: Department of Respiratory Medicine and Critical Care Medicine, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors: Guo-Xiong
authors: GX
authors: Luo
authors: Department of Respiratory Medicine and Critical Care Medicine, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors: Xue
authors: X
authors: Zeng
authors: Department of Respiratory Medicine and Critical Care Medicine, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors: Li-Li
authors: LL
authors: Lan
authors: Department of Infectious Disease, Institute of Infectious Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors: Qin
authors: Q
authors: Ning
authors: Department of Respiratory Medicine and Critical Care Medicine, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors: Yong-Jian
authors: YJ
authors: Xu
authors: Department of Respiratory Medicine and Critical Care Medicine, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors: Jian-Ping
authors: JP
authors: Zhao
authors: Department of Respiratory Medicine and Critical Care Medicine, Huazhong University of Science and Technology, Wuhan, 430030, China. xiejjgg@hotmail.com.
authors: Jun-Gang
authors: JG
authors: Xie
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D017209:Apoptosis; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000166:cytology / Q000187:drug effects*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism; D012906:Smoke / Q000009:adverse effects*; D014026:Nicotiana / Q000633:toxicity*; D014407:Tumor Cells, Cultured
chemical_list: D018840:HSP70 Heat-Shock Proteins; D012906:Smoke; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine