title: Mechanical properties of cancer cytoskeleton depend on actin filaments to microtubules content: investigating different grades of colon cancer cell lines.
abstract: Biomechanical properties of cancer cells have been proposed as promising biomarkers to investigate cancer progression. Cytoskeletal reorganization alters these characteristics in different grades of cancer cells. In the present study based on the micropipette aspiration method, whole body evaluation for two different colon cancer cells was performed to determine viscoelastic parameters of the cells. A finite element model was developed for verification of experiments and predicting some behaviors of cells. Western blot analysis and fluorescence intensity for actin microfilaments and microtubules were performed to measure cell content of the proteins. It was illustrated that the proportion of microtubules and actin microfilaments is different in grade I and grade IV colon cancer cells in a manner that microtubules attain an effectual role in progressive reorganization of cytoskeleton in transition from nonaggressive to malignant phenotypes in cancer cells. Furthermore, it was concluded that larger instantaneous Young's modulus value for high grade cells is related to the existence of extensively build-up actin networks at the cell cortex. Based on the cell mechanics results, a simple parameter is suggested for sorting different grades of colon cancer cells.
authors: Department of Mechanical Engineering, Iran University of Science and Technology, Tehran, Iran.
authors: M
authors: M
authors: Pachenari
authors: Department of Mechanical Engineering, Iran University of Science and Technology, Tehran, Iran.
authors: S M
authors: SM
authors: Seyedpour
authors: Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Taleghani Hospital, Parvaneh St., Velenjak, 1985717444 Tehran, Iran. Electronic address: janmaleki@sbmu.ac.ir.
authors: M
authors: M
authors: Janmaleki
authors: Department of Mechanical Engineering, Tarbiat Modares University, Tehran, Iran.
authors: S
authors: S
authors: Babazadeh Shayan
authors: Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Taleghani Hospital, Parvaneh St., Velenjak, 1985717444 Tehran, Iran.
authors: S
authors: S
authors: Taranejoo
authors: Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan.
authors: H
authors: H
authors: Hosseinkhani
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000199:Actins / Q000378:metabolism; D015766:Albendazole / Q000494:pharmacology; D015153:Blotting, Western; D002465:Cell Movement / Q000187:drug effects; D003110:Colonic Neoplasms / Q000473:pathology*; D003599:Cytoskeleton / Q000473:pathology*; D055119:Elastic Modulus / Q000502:physiology; D004548:Elasticity; D020342:Finite Element Analysis; D019073:HT29 Cells; D006801:Humans; D008870:Microtubules / Q000378:metabolism* / Q000473:pathology; D014783:Viscosity
chemical_list: D000199:Actins; D015766:Albendazole
keywords: Cancer cell mechanics; Finite element analysis; Micropipette aspiration; Rate of deformability