title: Cloning and characterization of a protein elicitor Sm1 gene from Trichoderma harzianum.
abstract: A small protein, cysteine-rich, designated SM1, produced by Trichoderma virens and Trichoderma atroviride, acts as elicitor for triggering plant defense reactions. We analyzed Sm1 gene expression of eight different strains of Trichoderma spp. grown on glucose, seeds or roots of beans. Regardless of the carbon source, T37 strain had significantly higher Sm1 expression and was chosen for further studies. When grown on different carbon sources, Sm1 expression was highest on galactose, bean seed, glucose and starch. Sm1 gene from T37 strain was cloned; it had a single exon, and encoded a protein of 138 amino acids, showing high sequence identity with some proteins belonging to the cerato-platanin family.
authors: Department of Biochemistry and Molecular Biology, Federal University of Goiás (ICB II), Goiânia, GO, 74690-900, Brazil.
authors: Rachel Silveira
authors: RS
authors: Freitas
authors: Andrei Stecca
authors: AS
authors: Steindorff
authors: Marcelo Henrique Soller
authors: MH
authors: Ramada
authors: Saulo José Linhares
authors: SJ
authors: de Siqueira
authors: Eliane Ferreira
authors: EF
authors: Noronha
authors: Cirano José
authors: CJ
authors: Ulhoa
mesh_terms: D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014242:Trichoderma / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins