title: Overexpression, crystallization and preliminary X-ray crystallographic analysis of glucuronoxylan xylanohydrolase (Xyn30A) from Clostridium thermocellum.
abstract: The modular carbohydrate-active enzyme belonging to glycoside hydrolase family 30 (GH30) from Clostridium thermocellum (CtXynGH30) is a cellulosomal protein which plays an important role in plant cell-wall degradation. The full-length CtXynGH30 contains an N-terminal catalytic module (Xyn30A) followed by a family 6 carbohydrate-binding module (CBM6) and a dockerin at the C-terminus. The recombinant protein has a molecular mass of 45 kDa. Preliminary structural characterization was carried out on Xyn30A crystallized in different conditions. All tested crystals belonged to space group P1 with one molecule in the asymmetric unit. Molecular replacement has been used to solve the Xyn30A structure.
authors: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781 039, India.
authors: Anil Kumar
authors: AK
authors: Verma
authors: Arun
authors: A
authors: Goyal
authors: Filipe
authors: F
authors: Freire
authors: Pedro
authors: P
authors: Bule
authors: Immacolata
authors: I
authors: Venditto
authors: Joana L A
authors: JL
authors: Brás
authors: Helena
authors: H
authors: Santos
authors: Vânia
authors: V
authors: Cardoso
authors: Cecília
authors: C
authors: Bonifácio
authors: Andrew
authors: A
authors: Thompson
authors: Maria João
authors: MJ
authors: Romão
authors: José A M
authors: JA
authors: Prates
authors: Luís M A
authors: LM
authors: Ferreira
authors: Carlos M G A
authors: CM
authors: Fontes
authors: Shabir
authors: S
authors: Najmudin
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D048013:Acetivibrio thermocellus / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006639:Histidine / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014995:Xylosidases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C471213:His-His-His-His-His-His; D009842:Oligopeptides; D011993:Recombinant Fusion Proteins; D006639:Histidine; D014995:Xylosidases; C068045:glucuronoxylan xylanohydrolase
keywords: Clostridium thermocellum; Xyn30A; glycoside hydrolase family 30