title: The combined expression patterns of Ikaros isoforms characterize different hematological tumor subtypes.
abstract: A variety of genetic alterations are considered hallmarks of cancer development and progression. The Ikaros gene family, encoding for key transcription factors in hematopoietic development, provides several examples as genetic defects in these genes are associated with the development of different types of leukemia. However, the complex patterns of expression of isoforms in Ikaros family genes has prevented their use as clinical markers. In this study, we propose the use of the expression profiles of the Ikaros isoforms to classify various hematological tumor diseases. We have standardized a quantitative PCR protocol to estimate the expression levels of the Ikaros gene exons. Our analysis reveals that these levels are associated with specific types of leukemia and we have found differences in the levels of expression relative to five interexonic Ikaros regions for all diseases studied. In conclusion, our method has allowed us to precisely discriminate between B-ALL, CLL and MM cases. Differences between the groups of lymphoid and myeloid pathologies were also identified in the same way.
authors: Laboratory of Human Genetics, Los Andes University, Bogotá, Colombia.
authors: Carlos A
authors: CA
authors: Orozco
authors: Andrés
authors: A
authors: Acevedo
authors: Lazaro
authors: L
authors: Cortina
authors: Gina E
authors: GE
authors: Cuellar
authors: Mónica
authors: M
authors: Duarte
authors: Liliana
authors: L
authors: Martín
authors: Néstor M
authors: NM
authors: Mesa
authors: Javier
authors: J
authors: Muñoz
authors: Carlos A
authors: CA
authors: Portilla
authors: Sandra M
authors: SM
authors: Quijano
authors: Guillermo
authors: G
authors: Quintero
authors: Miriam
authors: M
authors: Rodriguez
authors: Carlos E
authors: CE
authors: Saavedra
authors: Helena
authors: H
authors: Groot
authors: María M
authors: MM
authors: Torres
authors: Valeriano
authors: V
authors: López-Segura
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017398:Alternative Splicing; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D018450:Disease Progression; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D019337:Hematologic Neoplasms / Q000175:diagnosis / Q000235:genetics*; D006801:Humans; D051740:Ikaros Transcription Factor / Q000235:genetics*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics; D008875:Middle Aged; D005810:Multigene Family; D009928:Organ Specificity / Q000235:genetics; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics; D020033:Protein Isoforms; D059467:Transcriptome; D055815:Young Adult
chemical_list: D020033:Protein Isoforms; D051740:Ikaros Transcription Factor