title: In-line FINCH super resolution digital holographic fluorescence microscopy using a high efficiency transmission liquid crystal GRIN lens.
abstract: We report a new optical arrangement that creates high-efficiency, high-quality Fresnel incoherent correlation holography (FINCH) holograms using polarization sensitive transmission liquid crystal gradient index (TLCGRIN) diffractive lenses. In contrast, current universal practice in the field employs a reflective spatial light modulator (SLM) to separate sample and reference beams. Polarization sensitive TLCGRIN lenses enable a straight optical path, have >90% transmission efficiency, are not pixilated, and are free of many limitations of reflective SLM devices. For each sample point, two spherical beams created by a glass lens in combination with a polarization sensitive TLCGRIN lens interfere and create a hologram and resultant super resolution image.
authors: Gary
authors: G
authors: Brooker
authors: Nisan
authors: N
authors: Siegel
authors: Joseph
authors: J
authors: Rosen
authors: Nobuyuki
authors: N
authors: Hashimoto
authors: Makoto
authors: M
authors: Kurihara
authors: Ayano
authors: A
authors: Tanabe
mesh_terms: D006696:Holography / Q000295:instrumentation*; D007909:Lenses; D050866:Liquid Crystals; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D011058:Pollen