title: Detection of papillomavirus in equine periocular and penile squamous cell carcinoma.
abstract: Squamous cell carcinoma (SCC) is the most common tumor arising in the periocular and penile areas of horses. Both ultraviolet radiation and papillomaviruses have been implicated in the pathogenesis of SCC in various species, including the horse. This retrospective study used polymerase chain reaction (PCR) to detect papillomavirus DNA in archival biopsy samples from equine periocular and penile SCC from 3 different geographic areas (northeast, southeast, and central United States). Forty-two periocular SCCs were tested; none contained papillomavirus DNA. Twenty-two penile SCCs were tested, and papillomavirus DNA was identified in 10 (43%) cases. Sequencing of the PCR products revealed homology with Equus caballus papillomavirus 2 (EcPV-2). No geographic distribution in the detection of papillomavirus was identified. Penile SCCs were significantly more likely to be papillomavirus positive than the periocular SCCs (P < 0.001). The role of papillomavirus in the development of penile SCC requires further investigation. The differing pathogeneses of periocular and penile SCC suggest that the tumors may respond differently to treatment.
authors: 1Kim M. Newkirk, 2407 River Drive, Room A205, Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996. knewkirk@utk.edu.
authors: Kim M
authors: KM
authors: Newkirk
authors: Diane V H
authors: DV
authors: Hendrix
authors: Eman A
authors: EA
authors: Anis
authors: Barton W
authors: BW
authors: Rohrbach
authors: Eugene J
authors: EJ
authors: Ehrhart
authors: Jerry A
authors: JA
authors: Lyons
authors: Stephen A
authors: SA
authors: Kania
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D006734:Horse Diseases / Q000473:pathology* / Q000821:virology; D006736:Horses; D008297:Male; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D010412:Penile Neoplasms / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012189:Retrospective Studies; D012878:Skin Neoplasms / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D014481:United States
chemical_list: D004279:DNA, Viral
keywords: Equine; papillomavirus; penile; periocular; squamous cell carcinoma