title: Anti-inflammatory effect of platelet-rich plasma on nucleus pulposus cells with response of TNF-α and IL-1.
abstract: The purpose of this study was to investigate the anti-inflammatory effect of platelet-rich plasma (PRP) with collagen matrix on human nucleus pulposus (NP) cell in response to pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-α) and interleukin-1 (IL-1). NP cells from human disks were cultured in a monolayer and maintained in the collagen matrix prior to the addition of recombinant human IL-1 and TNF-α. After applying IL-1 and TNF-α, PRP prepared by using a commercially available platelet concentration system was added. The response was investigated using real-time PCR for mRNA expression of type II collagen, aggrecan, matrix metalloproteinase-3 (MMP-3), and cyclooxygenase-2 (COX-2). The combination of IL-1β and TNF-α led to decrease of matrix synthesis gene expression such as collagen type II and aggrecan and increase of the degradation gene expression of COX-2 and MMP-3, compared to the control. Consecutive PRP exposure significantly recovered the down-regulated gene expression of collagen type II and aggrecan and significantly reduced the increased MMP-3 and COX-2 gene expression, compared to that of control groups with pro-inflammatory cytokines. The administration of PRP with collagen matrix markedly suppressed cytokine-induced pro-inflammatory degrading enzymes and mediators in the NP cell. It also rescued gene expression concerning matrix synthesis, thereby stabilizing NP cell differentiation.
authors: Department of Orthopaedic Surgery, Seoul National University College of Medicine, 166 Gumiro, Bundang-gu, Sungnam, 463-707, Republic of Korea; Spine Center, Seoul National University Bundang Hospital, 166 Gumiro, Bundang-gu, Sungnam, 463-707, Republic of Korea.
authors: Ho-Joong
authors: HJ
authors: Kim
authors: Jin S
authors: JS
authors: Yeom
authors: Yong-Gon
authors: YG
authors: Koh
authors: Jee-Eun
authors: JE
authors: Yeo
authors: Kyoung-Tak
authors: KT
authors: Kang
authors: Young-Mi
authors: YM
authors: Kang
authors: Bong-Soon
authors: BS
authors: Chang
authors: Choon-Ki
authors: CK
authors: Lee
mesh_terms: D024043:Collagen Type II / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D051546:Cyclooxygenase 2 / Q000096:biosynthesis / Q000378:metabolism; D015536:Down-Regulation / Q000502:physiology; D064368:Healthy Volunteers; D006801:Humans; D053583:Interleukin-1beta / Q000502:physiology*; D055959:Intervertebral Disc Degeneration / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D019278:Matrix Metalloproteinase 3 / Q000096:biosynthesis / Q000378:metabolism; D008875:Middle Aged; D053657:Platelet-Rich Plasma / Q000737:chemistry / Q000166:cytology; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
chemical_list: D024043:Collagen Type II; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3
keywords: interleukin-1; nucleus pulposus cell; platelet-rich plasma; tumor necrosis factor-α