title: An artificial receptor fabricated by target recognition determinant imprinting for selective capture of α-amanitin.
abstract: This is the first reported work of artificial α-amanitin receptors which are prepared using the design and synthesis of a template molecule based on an α-amanitin recognition determinant imprinting strategy. The resultant molecularly imprinted polymers (MIPs) are evaluated using high performance liquid chromatography (HPLC), binding experiment, nitrogen adsorption measurement, and solid-phase extraction. Experiment clearly demonstrates that the MIPs as the HPLC stationary phase can specifically recognize α-amanitin from analogues. The MIPs also successfully adsorb trace amounts of α-amanitin in spiked serum samples selectively pretreated and enriched through molecularly imprinted solid phase extraction. The limit of detection and recovery is 3.0 ng mL(-1) and 88.5-95.9%, respectively, for α-amanitin in serum samples. The high specific adsorption and excellent selectivity of the MIPs arises from imprinting effects related to the imprinting cavity of the polymeric matrix, the metal-coordination bond, and the hydrogen bond between the receptor and ligand.
authors: Department of Chemistry, South China Normal University, Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, Guangzhou 510006, China.
authors: Yongxian
authors: Y
authors: Li
authors: Department of Chemistry, South China Normal University, Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, Guangzhou 510006, China.
authors: Lei
authors: L
authors: Tan
authors: Department of Chemistry, South China Normal University, Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, Guangzhou 510006, China.
authors: He
authors: H
authors: Li
authors: Department of Chemistry, South China Normal University, Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, Guangzhou 510006, China.
authors: Zhiguang
authors: Z
authors: Xu
authors: Department of Chemistry, South China Normal University, Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, Guangzhou 510006, China.
authors: Xiongjun
authors: X
authors: Zuo
authors: Department of Chemistry, South China Normal University, Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, Guangzhou 510006, China. Electronic address: tanglab@scnu.edu.cn.
authors: Youwen
authors: Y
authors: Tang
mesh_terms: D053959:Alpha-Amanitin / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006860:Hydrogen Bonding; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry; D062165:Receptors, Artificial / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods
chemical_list: D053959:Alpha-Amanitin; D011108:Polymers; D062165:Receptors, Artificial
keywords: High performance liquid chromatography; Molecular imprinted polymers; Solid-phase extraction; α-Amanitin