title: Anti-tumor activity of three novel derivatives of ginsenoside on colorectal cancer cells.
abstract: 25-Hydroxyprotopanaxadiol (25-OH-PPD) is a natural compound isolated from Panax ginseng, and its anti-tumor activity has been studied in previous publication. In the current study, we investigated the anti-tumor activity of three novel derivatives synthesized from 25-OH-PPD, namely (20R)-12β-O-(l-chloracetyl)-dammarane-3β, 20, 25-triol (xl), (20R)-3β-O-(l-alanyl)-dammarane-12β, 20, 25-triol (1c), and (20R)-3β-O-(Boc-l-arginyl)-dammarane-12β, 20, 25-triol (8b). All three compounds significantly inhibited the growths of human colorectal cancer cells, while having lesser effect on the growth of normal primary muscle cells and spleno-lymphocytes. Further mechanistic study demonstrated that these compounds could induce apoptosis by activating the components of caspase-signaling pathways in HCT116 cells, but not in spleno-lymphocytes. Taken together, the results suggested that 25-OH-PPD derivatives exerted promising anti-tumor activity that is specific to human colorectal cancer cells, and may therefore represent a potential chemotherapeutic strategy for the treatment of colorectal cancer.
authors: College of Life Science, Liaoning University, Shenyang 110036, People's Republic of China.
authors: Xichun
authors: X
authors: Xia
authors: College of Life Science, Liaoning University, Shenyang 110036, People's Republic of China.
authors: Bowen
authors: B
authors: Jiang
authors: College of Life Science, Liaoning University, Shenyang 110036, People's Republic of China.
authors: Wei
authors: W
authors: Liu
authors: Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China; Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of Education, Yanbian University College of Pharmacy, Yanji 133002, People's Republic of China.
authors: Peng
authors: P
authors: Wang
authors: Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
authors: Yanhua
authors: Y
authors: Mou
authors: Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
authors: Yafei
authors: Y
authors: Liu
authors: Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China; Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of Education, Yanbian University College of Pharmacy, Yanji 133002, People's Republic of China. Electronic address: zyq4885@126.com.
authors: Yuqing
authors: Y
authors: Zhao
authors: College of Life Science, Liaoning University, Shenyang 110036, People's Republic of China. Electronic address: xiulibi@gmail.com.
authors: Xiuli
authors: X
authors: Bi
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D036145:Ginsenosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D008968:Molecular Conformation; D005894:Panax / Q000737:chemistry*; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
chemical_list: C529607:25-hydroxyprotopanaxadiol; D000972:Antineoplastic Agents, Phytogenic; D036145:Ginsenosides
keywords: Anti-tumor activity; Apoptosis; Chemotherapeutic; Derivatives of 25-OH-PPD