title: Comparative analysis of microRNAs from the lungs and trachea of dogs (Canis familiaris) infected with canine influenza virus.
abstract: MicroRNAs (miRNAs) are a class of endogenous non-coding small RNAs of 18-22-nucleotides in length that regulate gene expression at the post-transcriptional level. The objective of this study was to examine the differences in the miRNA expression profiles of the lungs and trachea of beagle dogs infected with canine influenza virus (CIV). Total RNA was isolated from lung and trachea tissues of beagle dogs infected and non-infected with H3N2 CIV at 4 dpi. A total of 41,512,315 and 39,107,475 reads were obtained from the lung and trachea, respectively. Out of a total 288 dog miRNAs available in miRBase, 227 and 236 miRNAs were detected in the infected (Fg) and the non-infected lungs (Fc), respectively, whereas 242 miRNAs were detected in both the infected (Qg) and the non-infected trachea (Qc). From these, 34 and 45 miRNAs were differentially expressed in the lungs and trachea between the infected and non-infected dogs, respectively. More miRNAs were highly expressed in the non-infected tissues than in the infected tissues. miR-143 was the most abundantly expressed miRNA in the four samples, followed by let-7. In total, 252, 234, 196 and 235 novel miRNAs were identified in the Fc, Fg, Qc, and Qg groups, respectively. To our knowledge, this is the first study examining the miRNA gene expression in CIV infected dogs using the Solexa sequencing approach. We have revealed the existence of a large number miRNAs that are affected by CIV infection as well as identified some potentially new miRNAs. These findings will help us better understand the host-CIV interaction and its relationship to pathogenesis, as well as contribute to the prevention and control of CIV.
authors: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, People's Republic of China. Electronic address: furong0812@163.com.
authors: Fu-Rong
authors: FR
authors: Zhao
authors: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China.
authors: Shuo
authors: S
authors: Su
authors: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, People's Republic of China.
authors: Dong-Hui
authors: DH
authors: Zhou
authors: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China.
authors: Pei
authors: P
authors: Zhou
authors: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China.
authors: Ting-Chuan
authors: TC
authors: Xu
authors: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China.
authors: Liang-Quan
authors: LQ
authors: Zhang
authors: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China.
authors: Nan
authors: N
authors: Cao
authors: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China.
authors: Wen-Bao
authors: WB
authors: Qi
authors: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China.
authors: Gui-Hong
authors: GH
authors: Zhang
authors: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China. Electronic address: shoujunli@scau.edu.cn.
authors: Shou-Jun
authors: SJ
authors: Li
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000235:genetics* / Q000473:pathology / Q000821:virology; D004285:Dogs; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D053122:Influenza A Virus, H3N2 Subtype / Q000502:physiology*; D008168:Lung / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D035683:MicroRNAs / Q000032:analysis*; D009976:Orthomyxoviridae Infections / Q000235:genetics / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D014132:Trachea / Q000378:metabolism* / Q000473:pathology / Q000821:virology
chemical_list: D035683:MicroRNAs
keywords: Canine influenza virus (CIV); Differentially expressed; High-throughput sequencing; MicroRNA (miRNA)