title: Crystallization and preliminary crystallographic analysis of a surface antigen glycoprotein, SAG19, from Eimeria tenella.
abstract: Coccidiosis in chickens is caused by the apicomplexan parasite Eimeria tenella and is thought to involve a role for a superfamily of more than 20 cysteine-rich surface antigen glycoproteins (SAGs) in host-parasite interactions. A representative member of the family, SAG19, has been overexpressed in Escherichia coli, purified and crystallized by the hanging-drop method of vapour diffusion using ammonium sulfate as the precipitant. Crystals of SAG19 diffracted to beyond 1.50 Å resolution and belonged to space group I4, with unit-cell parameters a = b = 108.2, c = 37.5 Å. Calculation of possible values of VM suggests that there is a single molecule in the asymmetric unit.
authors: Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, England.
authors: Nur Zazarina
authors: NZ
authors: Ramly
authors: Sergey N
authors: SN
authors: Rouzheinikov
authors: Svetlana E
authors: SE
authors: Sedelnikova
authors: Patrick J
authors: PJ
authors: Baker
authors: Yock Ping
authors: YP
authors: Chow
authors: Kiew Lian
authors: KL
authors: Wan
authors: Sheila
authors: S
authors: Nathan
authors: David W
authors: DW
authors: Rice
mesh_terms: D000595:Amino Acid Sequence; D000645:Ammonium Sulfate / Q000737:chemistry; D000954:Antigens, Surface / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D016786:Eimeria tenella / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D000954:Antigens, Surface; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D000645:Ammonium Sulfate
keywords: Eimeria tenella; SAG19; apicomplexa