title: Proteomic and genomic analyses of antimony resistant Leishmania infantum mutant.
abstract: BACKGROUND
  Antimonials remain the primary antileishmanial drugs in most developing countries. However, drug resistance to these compounds is increasing and our understanding of resistance mechanisms is partial.
  
  
  RESULTS
  In the present study, quantitative proteomics using stable isotope labelling of amino acids in cell culture (SILAC) and genome next generation sequencing were used in order to better characterize in vitro generated Leishmania infantum antimony resistant mutant (Sb2000.1). Using the proteomic method, 58 proteins were found to be differentially regulated in Sb2000.1. The ABC transporter MRPA (ABCC3), a known marker of antimony resistance, was observed for the first time in a proteomic screen. Furthermore, transfection of its gene conferred antimony resistance in wild-type cells. Next generation sequencing revealed aneuploidy for 8 chromosomes in Sb2000.1. Moreover, specific amplified regions derived from chromosomes 17 and 23 were observed in Sb2000.1 and a single nucleotide polymorphism (SNP) was detected in a protein kinase (LinJ.33.1810-E629K).
  
  
  CONCLUSIONS
  Our results suggest that differentially expressed proteins, chromosome number variations (CNVs), specific gene amplification and SNPs are important features of antimony resistance in Leishmania.
authors: Centre de Recherche en Infectiologie du Centre de Recherche du CHU de Québec, Pavillon CHUL, and Département de Microbiologie-Infectiologie et Immunologie, Faculté de Médecine, Université Laval, Québec, Canada.
authors: Marie-Christine
authors: MC
authors: Brotherton
authors: Sylvie
authors: S
authors: Bourassa
authors: Philippe
authors: P
authors: Leprohon
authors: Danielle
authors: D
authors: Légaré
authors: Guy G
authors: GG
authors: Poirier
authors: Arnaud
authors: A
authors: Droit
authors: Marc
authors: M
authors: Ouellette
mesh_terms: D000782:Aneuploidy; D000818:Animals; D000965:Antimony / Q000494:pharmacology*; D004351:Drug Resistance / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D018503:Genome, Protozoan; D018314:Leishmania infantum / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D020641:Polymorphism, Single Nucleotide; D040901:Proteomics; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D015800:Protozoan Proteins; D000965:Antimony