title: LIM homeobox transcription factor 1B is associated with pro-fibrotic components and apoptosis in hypoxia/reoxygenation renal tubular epithelial cells.
abstract: LIM homeobox transcription factor 1B (LMX1B) is a transcription factor of the LIM-homeodomain type, which plays an important role in foetal development during formation of the extremities, kidneys, eyes, and the brain. Furthermore, LMX1B has been implicated in nail-patella syndrome, which is predominantly characterized by malformation of limbs and nails, and in 30 % of patients, nephropathy, including renal fibrosis, is observed. Since no reports were available that studied the link between LMX1B expression and pro-fibrotic components and apoptosis in hypoxic renal tubular epithelial cells (RTEC), we explored if LMX1B was associated with extracellular matrix components, profibrotic factors, and apoptosis induced by hypoxia/reoxygenation (H-R). In this cell system under hypoxic conditions, when the expression of LMX1B was inhibited in H-R RTEC, the expression of transforming growth factor-βl, collagen-III, fibronectin, cleaved caspase-3, and cell apoptosis rate was increased. Consequently, overexpression of LMX1B was associated with reduced cell apoptosis, whilst downregulation of LMX1B was associated with increased cell apoptosis in H-R RTEC.
authors: Department of Nephrology, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510655, China, a126tianbiao@126.com.
authors: Tian-Biao
authors: TB
authors: Zhou
authors: Hui-Ling
authors: HL
authors: Xu
authors: Yuan-Han
authors: YH
authors: Qin
authors: Feng-Ying
authors: FY
authors: Lei
authors: Wei-Fang
authors: WF
authors: Huang
authors: Gregor P C
authors: GP
authors: Drummen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D015687:Cell Hypoxia; D002460:Cell Line; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology*; D005109:Extracellular Matrix / Q000378:metabolism*; D005355:Fibrosis; D007684:Kidney Tubules / Q000378:metabolism / Q000473:pathology*; D060850:LIM-Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress; D051381:Rats; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C112149:LIM homeobox transcription factor 1 beta; D060850:LIM-Homeodomain Proteins; D014157:Transcription Factors