title: A conserved KIN17 curved DNA-binding domain protein assembles with SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE7 to adapt Arabidopsis growth and development to limiting copper availability.
abstract: Proper copper (Cu) homeostasis is required by living organisms to maintain essential cellular functions. In the model plant Arabidopsis (Arabidopsis thaliana), the SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE7 (SPL7) transcription factor participates in reprogramming global gene expression during Cu insufficiency in order to improve the metal uptake and prioritize its distribution to Cu proteins of major importance. As a consequence, spl7 null mutants show morphological and physiological disorders during Cu-limited growth, resulting in lower fresh weight, reduced root elongation, and chlorosis. On the other hand, the Arabidopsis KIN17 homolog belongs to a well-conserved family of essential eukaryotic nuclear proteins known to be stress activated and involved in DNA and possibly RNA metabolism in mammals. In the study presented here, we uncovered that Arabidopsis KIN17 participates in promoting the Cu deficiency response by means of a direct interaction with SPL7. Moreover, the double mutant kin17-1 spl7-2 displays an enhanced Cu-dependent phenotype involving growth arrest, oxidative stress, floral bud abortion, and pollen inviability. Taken together, the data presented here provide evidence for SPL7 and KIN17 protein interaction as a point of convergence in response to both Cu deficiency and oxidative stress.
authors: Department of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.
authors: Antoni
authors: A
authors: Garcia-Molina
authors: Shuping
authors: S
authors: Xing
authors: Peter
authors: P
authors: Huijser
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects*; D017360:Arabidopsis / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001483:Base Sequence; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D017124:Conserved Sequence; D003300:Copper / Q000633:toxicity*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D008954:Models, Biological; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009687:Nuclear Proteins / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010641:Phenotype; D063245:Plant Development / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D011058:Pollen / Q000187:drug effects / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D014026:Nicotiana / Q000187:drug effects / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C000592238:KIN17 protein, Arabidopsis; D009687:Nuclear Proteins; C483288:SPL7 protein, Arabidopsis; D014157:Transcription Factors; D003300:Copper