title: Hyperoside regulates the level of thymic stromal lymphopoietin through intracellular calcium signalling.
abstract: Hyperoside (HYP) is the principle active component of Crataegus pinnatifida. Thymic stromal lymphopoietin (TSLP) plays a vital role in the pathogenesis of allergic reactions. Here, we investigated how HYP regulates the levels of TSLP in a human mast cell line, HMC-1 cells. We analyzed the levels of TSLP by treatment with HYP in phorbol myristate acetate plus calcium ionophore A23187-stimulated HMC-1 cells with ELISA and a polymerase chain reaction analysis. We also analyzed the pathway that HYP regulates TSLP by measuring the level of fluorescent intracellular calcium and using a Western blot analysis. HYP decreased the level of intracellular calcium in stimulated HMC-1 cells. It also significantly decreased the production and mRNA expression of TSLP in stimulated HMC-1 cells. It significantly decreased the levels of receptor-interacting protein 2 and active caspase-1 in stimulated HMC-1 cells. HYP significantly decreased the translocation of NF-κB into the nucleus and degradation of IκBα in the cytoplasm in stimulated HMC-1 cells. Furthermore, it significantly decreased the production and mRNA expression of interleukin-1β and interleukin-6 in stimulated HMC-1 cells. Taken together, our findings establish HYP as a potential agent for the treatment of allergic reactions.
authors: Department of Pharmacology, College of Korean Medicine, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Korea.
authors: Na-Ra
authors: NR
authors: Han
authors: Ji-Hyun
authors: JH
authors: Go
authors: Hyung-Min
authors: HM
authors: Kim
authors: Hyun-Ja
authors: HJ
authors: Jeong
mesh_terms: D000001:Calcimycin; D002118:Calcium / Q000378:metabolism*; D061207:Calcium Ionophores; D020013:Calcium Signaling; D020170:Caspase 1 / Q000378:metabolism; D002460:Cell Line; D027825:Crataegus / Q000737:chemistry; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2 / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D061207:Calcium Ionophores; D016207:Cytokines; D053583:Interleukin-1beta; D015850:Interleukin-6; D016328:NF-kappa B; D000001:Calcimycin; C021304:hyperoside; D011794:Quercetin; C506253:RIPK2 protein, human; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2; D020170:Caspase 1; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium; D000094632:Thymic Stromal Lymphopoietin
keywords: caspase-1; hyperoside; intracellular calcium; mast cell; thymic stromal lymphopoietin