title: Phylogenetic analysis of ruminant Theileria spp. from China based on 28S ribosomal RNA gene.
abstract: Species identification using DNA sequences is the basis for DNA taxonomy. In this study, we sequenced the ribosomal large-subunit RNA gene sequences (3,037-3,061 bp) in length of 13 Chinese Theileria stocks that were infective to cattle and sheep. The complete 28S rRNA gene is relatively difficult to amplify and its conserved region is not important for phylogenetic study. Therefore, we selected the D2-D3 region from the complete 28S rRNA sequences for phylogenetic analysis. Our analyses of 28S rRNA gene sequences showed that the 28S rRNA was useful as a phylogenetic marker for analyzing the relationships among Theileria spp. in ruminants. In addition, the D2-D3 region was a short segment that could be used instead of the whole 28S rRNA sequence during the phylogenetic analysis of Theileria, and it may be an ideal DNA barcode.
authors: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Key Laboratory of Grazing Animal Diseases MOA, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu 730046, P. R. China.
authors: Huitian
authors: H
authors: Gou
authors: Guiquan
authors: G
authors: Guan
authors: Miling
authors: M
authors: Ma
authors: Aihong
authors: A
authors: Liu
authors: Zhijie
authors: Z
authors: Liu
authors: Zongke
authors: Z
authors: Xu
authors: Qiaoyun
authors: Q
authors: Ren
authors: Youquan
authors: Y
authors: Li
authors: Jifei
authors: J
authors: Yang
authors: Ze
authors: Z
authors: Chen
authors: Hong
authors: H
authors: Yin
authors: Jianxun
authors: J
authors: Luo
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D012418:Ruminants; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000662:veterinary; D016794:Theileria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D013801:Theileriasis / Q000469:parasitology*
chemical_list: D004275:DNA, Ribosomal; D012339:RNA, Ribosomal, 28S
keywords: 28S rRNA; China; Theileria sp.; cattle; phylogeny; sheep