title: Adsorption behavior of proteins on temperature-responsive resins.
abstract: The adsorption behavior of proteins on thermo-responsible resins based on poly(N-isopropylacrylamide) and its copolymer containing an anionic co-monomer has been investigated. The influence of the polymer composition, i.e., the content of the co-monomer and crosslinker on the thermo-sensitivity of the protein adsorption has been quantified. The properties of ungrafted polymer as well grafted onto the agarose matrix have been analyzed and compared. Batch and dynamic (column) experiments have been performed to measure the adsorption equilibrium of proteins and to quantify the phase transition process. As model proteins lysozyme, lactoferrin, α-chymotrypsinogen A and ovalbumin have been used. The adsorption process was found to be governed by ionic interactions between the negatively charged surface of resin and the protein, which enabled separation of proteins differing in electrostatic charge. The interactions enhanced with increase of temperature. Decrease of temperature facilitated desorption of proteins and reduced the salt usage in the desorption buffer. Grafted polymers exhibited markedly higher mechanical stability and, however, weaker temperature response compared to the ungrafted ones.
authors: Department of Chemical and Process Engineering, Rzeszów University of Technology, Powstańców Warszawy Ave. 6, 35-959 Rzeszów, Poland.
authors: Izabela
authors: I
authors: Poplewska
authors: Department of Chemical and Process Engineering, Rzeszów University of Technology, Powstańców Warszawy Ave. 6, 35-959 Rzeszów, Poland.
authors: Renata
authors: R
authors: Muca
authors: Institute of Macromolecular Chemistry, v.v.i., Academy of Sciences of the Czech Republic, Heyrovskeho nam. 2, CZ-162 00 Praha, Czech Republic.
authors: Adam
authors: A
authors: Strachota
authors: Department of Chemical and Process Engineering, Rzeszów University of Technology, Powstańców Warszawy Ave. 6, 35-959 Rzeszów, Poland.
authors: Wojciech
authors: W
authors: Piątkowski
authors: Department of Chemical and Process Engineering, Rzeszów University of Technology, Powstańców Warszawy Ave. 6, 35-959 Rzeszów, Poland. Electronic address: dorota.antos@prz.edu.pl.
authors: Dorota
authors: D
authors: Antos
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000327:Adsorption; D000818:Animals; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D044367:Phase Transition; D016062:Porosity; D011506:Proteins / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D011506:Proteins; C052970:poly-N-isopropylacrylamide
keywords: Bioseparations; N-Isopropylacrylamide; Thermo-responsible resins