title: Rapid emergence of novel GII.4 sub-lineages noroviruses associated with outbreaks in Huzhou, China, 2008-2012.
abstract: Infection caused by noroviruses (NoVs) is one of the most important causes of acute gastroenteritis in humans worldwide. To gain insight into the epidemiology of and genetic variation in NoV strains, stool samples collected from 18 outbreaks of acute gastroenteritis in Huzhou, China, between January 2008 and December 2012 were analyzed. Samples were tested for NoVs by real-time RT-PCR. Partial sequences of the RNA- dependent RNA polymerase (RdRp) and capsid gene of the positive samples were amplified by RT-PCR, and the PCR products were sequenced and used for phylogenetic analysis. NoVs were found to be responsible of 88.8% of all nonbacterial acute gastroenteritis outbreaks in Huzhou over the last 5 years. Genogroup II outbreaks largely predominated and represented 93% of all outbreaks. A variety of genotypes were found among genogroups I and II, including GI.4, GI.8, GII.4, and GII.b. Moreover, phylogenetic analyses identified two recombinant genotypes (polymerase/capsid): GI.2/GI.6 and GII.e/GII.4 2012 Sydney. GII.4 was predominant and involved in 8/10 typed outbreaks. During the study period, GII.4 NoV variants 2006b, New Orleans 2009, and Sydney 2012 were identified. This is the first report of the detection of GII.4 New Orleans 2009 variant, GII.e/GII.4 Sydney 2012 recombinant in outbreaks of acute gastroenteritis in China.
authors: Huzhou Center for Disease Control and Prevention, Huzhou, Zhejiang Province, China.
authors: Lei
authors: L
authors: Ji
authors: Xiaofang
authors: X
authors: Wu
authors: Wenting
authors: W
authors: Yao
authors: Liping
authors: L
authors: Chen
authors: Deshun
authors: D
authors: Xu
authors: Yuehua
authors: Y
authors: Shen
authors: Jiayu
authors: J
authors: Shen
authors: Jiankang
authors: J
authors: Han
mesh_terms: D017250:Caliciviridae Infections / Q000453:epidemiology* / Q000266:history; D002681:China / Q000453:epidemiology; D004196:Disease Outbreaks; D005759:Gastroenteritis / Q000453:epidemiology* / Q000266:history; D005838:Genotype; D049674:History, 21st Century; D006801:Humans; D008969:Molecular Sequence Data; D029322:Norovirus / Q000145:classification* / Q000235:genetics*; D016366:Open Reading Frames; D010802:Phylogeny; D012367:RNA, Viral; D011995:Recombination, Genetic
chemical_list: D012367:RNA, Viral