title: Aptamer-based biosensor for detection of phenylalanine at physiological pH.
abstract: A simple, sensitive aptamer-based biosensor for the detection of phenylalanine is developed using the electrochemical transduction method. For this proposed aptasensor, a 5-thiol-terminated aptamer is covalently attached onto a gold electrode. At the first time, the electrode was evaluated as an electrochemical aptasensor for determination of phenylalanine in aqueous solutions. This sensor was tested in a Tris-HCl buffer with physiological pH = 7.4 by cyclic voltammetry and differential pulse voltammetry. The detection limit and sensitivity of the modified electrode toward phenylalanine were estimated to be 1 nM (S/N = 3) and 0.367 μA nM(-1), respectively. The linear range of the signal was observed between 1 and 10 nM of phenylalanine with 0.9914 correlation factor. The herein-described approach is expected to promote the exploitation of aptamer-based biosensors for protein assays in biochemical and biomedical studies.
authors: Biochemistry Department, Genetic and Metabolism Group, Pasteur Institute of Iran, P.O. Box 13164, Tehran, Iran, skandar@pasteur.ac.ir.
authors: Eskandar
authors: E
authors: Omidinia
authors: Nasrin
authors: N
authors: Shadjou
authors: Mohammad
authors: M
authors: Hasanzadeh
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D004566:Electrodes; D006046:Gold; D006863:Hydrogen-Ion Concentration; D010649:Phenylalanine / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D010649:Phenylalanine; D006046:Gold