title: Cholinergic neuromuscular transmission mediated by interstitial cells of Cajal in the myenteric layer in mouse ileal longitudinal smooth muscles.
abstract: To elucidate the roles played by the interstitial cells of Cajal in the myenteric layer (ICC-MY) in cholinergic neuromuscular transmission, we recorded mechanical and electrical activities in response to electrical field stimulation (EFS) of the ileal longitudinal muscle strips from WBB6F1-W/W(V) (W/W(V)) mutant mice, that lacked ICC-MY and compared with those in WBB6F1-+/+ (+/+) control mice. In +/+ muscle strips, EFS induced phasic contractions, which were abolished or strongly attenuated by atropine or tetrodotoxin. In W/W(V) preparations, EFS induced similar phasic contractions, but the cholinergic component was smaller than that in +/+ strips. This was despite of the fact that the contractions because of exogenous applications of carbachol and high K(+) solution in W/W(V) strips were comparable to or rather greater than those in the +/+ preparations. EFS induced atropine-sensitive excitatory junction potentials (EJPs) in the +/+ longitudinal smooth muscle cells but not in W/W(V) cells. In the presence of eserine, EFS induced atropine-sensitive EJPs in W/W(V) cells. These results suggest that ICC-MY mediate the cholinergic neuromuscular transmission in mouse ileal longitudinal smooth muscles. In addition, the other pathway in which ICC-MY are not involved can operate concomitantly.
authors: Department of Animal Medical Sciences, Faculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-Ku, Kyoto, 603-8555, Japan, ytanavet@cc.kyoto-su.ac.jp.
authors: Yasuyuki
authors: Y
authors: Tanahashi
authors: Yoshirou
authors: Y
authors: Ichimura
authors: Kaori
authors: K
authors: Kimura
authors: Hayato
authors: H
authors: Matsuyama
authors: Satoshi
authors: S
authors: Iino
authors: Seiichi
authors: S
authors: Komori
authors: Toshihiro
authors: T
authors: Unno
mesh_terms: D018663:Adrenergic Agents / Q000494:pharmacology; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D018678:Cholinergic Agents / Q000494:pharmacology; D004558:Electric Stimulation; D006145:Guanethidine / Q000494:pharmacology; D007082:Ileum / Q000294:innervation / Q000502:physiology*; D066298:In Vitro Techniques; D056885:Interstitial Cells of Cajal / Q000502:physiology*; D008297:Male; D008564:Membrane Potentials; D051379:Mice; D008817:Mice, Mutant Strains; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000294:innervation / Q000502:physiology*; D009197:Myenteric Plexus / Q000502:physiology*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009469:Neuromuscular Junction / Q000502:physiology; D010830:Physostigmine / Q000494:pharmacology; D009435:Synaptic Transmission
chemical_list: D018663:Adrenergic Agents; D018678:Cholinergic Agents; D001285:Atropine; D010830:Physostigmine; D019331:NG-Nitroarginine Methyl Ester; D006145:Guanethidine