title: [Role of bone marrow-derived mesenchymal stem cells in treating estrogen deficiency induced osteoporosis].
abstract: OBJECTIVE
  To investigate the therapeutic effects of bone marrow-derived mesenchymal stem cells (BMSCs) from C57BL/6 mice on estrogen deficiency induced osteoporosis.
  
  
  METHODS
  Mouse models of estrogen deficiency induced osteoporosis were set up through ovariectomy (OVX) operation and sham operation group was set up as controls. BMSCs were injected via caudal veins. Micro-CT scanning of the femurs was conducted to detect the therapeutic effects of BMSCs. ELISA was used to test the expression level of TNF-α in serum before and after the injection of BMSCs. In the meantime, T cell apoptosis was also tested by flow cytometry combined with FITC-annexin V/7-amino actimycin D staining.
  
  
  RESULTS
  Compared with the sham operation group, the trabecular volume (BV/TV), bone mineral density (BMD) and trabecular number (Tb.N/mm) of osteoporosis mice set up by OVX were reduced significantly, and serum TNF-α was up-regulated a little. After the injection of BMSCs, the BV/TV, Tb.Th, Tb.N and T cell apoptosis in the osteoporosis mice increased, and the level of TNF-α decreased.
  
  
  CONCLUSIONS
  With the ability of immunoregulation, BMSCs might play a critical role in treating estrogen deficiency induced osteoporosis.
authors: Department of Endodontics, Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 400015, China.
authors: Yang
authors: Y
authors: Yu
authors: Bingyi
authors: B
authors: Shao
authors: Zhifei
authors: Z
authors: Zhou
authors: Fengqing
authors: F
authors: Shang
authors: Yi
authors: Y
authors: Shuai
authors: Xi
authors: X
authors: Wang
authors: Li
authors: L
authors: Liao
authors: Yan
authors: Y
authors: Jin
authors: Deqin
authors: D
authors: Yang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000166:cytology*; D004967:Estrogens / Q000172:deficiency*; D005260:Female; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D008810:Mice, Inbred C57BL; D010024:Osteoporosis / Q000097:blood / Q000000981:diagnostic imaging / Q000276:immunology / Q000601:surgery*; D013601:T-Lymphocytes / Q000166:cytology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D055114:X-Ray Microtomography
chemical_list: D004967:Estrogens; D014409:Tumor Necrosis Factor-alpha