title: RNA-Seq transcriptome profiling of upland cotton (Gossypium hirsutum L.) root tissue under water-deficit stress.
abstract: An RNA-Seq experiment was performed using field grown well-watered and naturally rain fed cotton plants to identify differentially expressed transcripts under water-deficit stress. Our work constitutes the first application of the newly published diploid D5 Gossypium raimondii sequence in the study of tetraploid AD1 upland cotton RNA-seq transcriptome analysis. A total of 1,530 transcripts were differentially expressed between well-watered and water-deficit stressed root tissues, in patterns that confirm the accuracy of this technique for future studies in cotton genomics. Additionally, putative sequence based genome localization of differentially expressed transcripts detected A2 genome specific gene expression under water-deficit stress. These data will facilitate efforts to understand the complex responses governing transcriptomic regulatory mechanisms and to identify candidate genes that may benefit applied plant breeding programs.
authors: USDA-ARS, Coastal Plains Soil, Water and Plant Research Center, Florence, South Carolina, United States of America.
authors: Megan J
authors: MJ
authors: Bowman
authors: Wonkeun
authors: W
authors: Park
authors: Philip J
authors: PJ
authors: Bauer
authors: Joshua A
authors: JA
authors: Udall
authors: Justin T
authors: JT
authors: Page
authors: Joshua
authors: J
authors: Raney
authors: Brian E
authors: BE
authors: Scheffler
authors: Don C
authors: DC
authors: Jones
authors: B Todd
authors: BT
authors: Campbell
mesh_terms: D000220:Adaptation, Biological / Q000235:genetics; D016000:Cluster Analysis; D055864:Droughts; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D003368:Gossypium / Q000235:genetics* / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D058977:Molecular Sequence Annotation; D018517:Plant Roots / Q000235:genetics* / Q000378:metabolism*; D017423:Sequence Analysis, RNA; D013312:Stress, Physiological / Q000235:genetics*; D059467:Transcriptome