title: Effect of ethanol extract of Selaginella doederleinii Hieron on the proliferation of nasopharyngeal carcinoma CNE-1 and C666-1 cells.
abstract: This paper mainly studied the effect of ethanol extract of Selaginella doederleinii Hieron on the proliferation of two kinds of nasopharyngeal carcinoma cell lines, CNE-1 and C666-1, and their mechanisms of action. Extract was obtained by heat reflux extraction with ethanol, and the effect of extract on the extracellular matrix adhesion of the cells, on their proliferation process, as well as on their colony-forming ability were tested using MTT assay. The results showed that the yield of dry extract was 36.4%. 2.5 g/ml extract in the high concentration group exhibited inhibitory activity that was directly proportional to the concentration on CNE-1 cells, while not exhibiting obvious proportional trend in respect with C666-1 cells. However, the inhibition rates against two types of cells can both reach between 30% ~ 50%. Under the effect of ethanol extract of Selaginella doederleinii Hieron, proliferative capacities of C666-1 and CNE-1 cells were affected to some extent.
authors: ENT Department, the First Affiliated Hospital of Xinxiang Medical College University, Xinxiang, China.
authors: Rong
authors: R
authors: Lian
authors: Jing
authors: J
authors: Li
authors: Hui-Min
authors: HM
authors: Ma
authors: Guo-Zheng
authors: GZ
authors: Zhang
authors: Xi-Ai
authors: XA
authors: Guo
authors: Xiao
authors: X
authors: Li
authors: Jian-Bin
authors: JB
authors: Yang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002277:Carcinoma; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D000431:Ethanol; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms; D032503:Selaginellaceae; D012997:Solvents; D014410:Tumor Stem Cell Assay
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D012997:Solvents; D000431:Ethanol
keywords: C666-1; CNE-1; Ethanol extract; Selaginella doederleinii Hieron