title: Chromosome territories reposition during DNA damage-repair response.
abstract: BACKGROUND
  Local higher-order chromatin structure, dynamics and composition of the DNA are known to determine double-strand break frequencies and the efficiency of repair. However, how DNA damage response affects the spatial organization of chromosome territories is still unexplored.
  
  
  RESULTS
  Our report investigates the effect of DNA damage on the spatial organization of chromosome territories within interphase nuclei of human cells. We show that DNA damage induces a large-scale spatial repositioning of chromosome territories that are relatively gene dense. This response is dose dependent, and involves territories moving from the nuclear interior to the periphery and vice versa. Furthermore, we have found that chromosome territory repositioning is contingent upon double-strand break recognition and damage sensing. Importantly, our results suggest that this is a reversible process where, following repair, chromosome territories re-occupy positions similar to those in undamaged control cells.
  
  
  CONCLUSIONS
  Thus, our report for the first time highlights DNA damage-dependent spatial reorganization of whole chromosomes, which might be an integral aspect of cellular damage response.
authors: Ishita S
authors: IS
authors: Mehta
authors: Mugdha
authors: M
authors: Kulashreshtha
authors: Sandeep
authors: S
authors: Chakraborty
authors: Ullas
authors: U
authors: Kolthur-Seetharam
authors: Basuthkar J
authors: BJ
authors: Rao
mesh_terms: D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D045584:Chromosome Positioning / Q000187:drug effects*; D002877:Chromosomes, Human / Q000187:drug effects* / Q000235:genetics; D002945:Cisplatin / Q000494:pharmacology; D004247:DNA / Q000235:genetics*; D004260:DNA Repair; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D007399:Interphase / Q000187:drug effects
chemical_list: D004247:DNA; D002945:Cisplatin