title: Antigenic analysis of monoclonal antibodies against different epitopes of σB protein of avian reovirus.
abstract: BACKGROUND
  Avian reovirus (ARV) causes arthritis, tenosynovitis, runting-stunting syndrome (RSS), malabsorption syndrome (MAS) and immunosuppression in chickens. σB is one of the major structural proteins of ARV, which is able to induce group-specific antibodies against the virus.
  
  
  RESULTS
  The present study described the identification of two linear B-cell epitopes in ARV σB through expressing a set of partially overlapping and consecutive truncated peptides spanning σB screened with two monoclonal antibodies (mAbs) 1F4 and 1H3-1.The data indicated that (21)KTPACW(26) (epitope A) and (32)WDTVTFH(38) (epitope B) were minimal determinants of the linear B cell epitopes. Antibodies present in the serum of ARV-positive chickens recognized the minimal linear epitopes in Western blot analyses. By sequence alignment analysis, we determined that the epitopes A and B were not conserved among ARV, duck reovirus (DRV) and turkey reovirus (TRV) strains. Western blot assays, confirmed that epitopes A and B were ARV-specific epitopes, and they could not react with the corresponding peptides of DRV and TRV.
  
  
  CONCLUSIONS
  We identified (21)KTPACW(26) and (32)WDTVTFH(38) as σB -specific epitopes recognized by mAbs 1F4 and 1H3-1, respectively. The results in this study may have potential applications in development of diagnostic techniques and epitope-based marker vaccines against ARV groups.
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, P. R. China.
authors: Chun-hong
authors: CH
authors: Yin
authors: Li-ting
authors: LT
authors: Qin
authors: Mei-yu
authors: MY
authors: Sun
authors: Yu-long
authors: YL
authors: Gao
authors: Xiao-le
authors: XL
authors: Qi
authors: Hong-lei
authors: HL
authors: Gao
authors: Yong-qiang
authors: YQ
authors: Wang
authors: Xiao-mei
authors: XM
authors: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003429:Cross Reactions; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry / Q000276:immunology*; D005260:Female; D051379:Mice; D030101:Orthoreovirus, Avian / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D018985:Epitopes, B-Lymphocyte