title: Expression of genes in normal fibroblast cells (WS1) in response to irradiation at 660nm.
abstract: Photobiomodulation has been found to increase gene expression and release of various growth factors and cytokines involved in wound healing. Photobiomodulation has been used to treat a wide variety of disorders, and has been found to be beneficial in the treatment of chronic wounds, however the exact underlying mechanism is not well understood. This study aimed to profile 84genes in response to irradiation at 660nm. WS1 human skin fibroblasts were used in gene expression profiling studies in response to irradiation with a 660nm diode laser at a fluence of 5J/cm(2) and power density of 11mW/cm(2). Forty-eight hours post-irradiation, 1μg RNA was reverse transcribed and used in real-time qualitative polymerase chain reaction (qPCR). Genes involved in the extracellular matrix and cell adhesion, inflammatory cytokines and chemokines, growth factors and signal transduction were evaluated. A total of 76genes were regulated by laser irradiation, 43genes were up-regulated while 33genes were down-regulated. Irradiation of WS1 cells at 660nm modulates the expression of genes involved in collagen production, cellular adhesion, remodelling and spreading, the cytoskeleton, inflammatory cytokines and chemokines, growth factors and molecules involved in signal transduction.
authors: Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, South Africa. Electronic address: nhoureld@uj.ac.za.
authors: Nicolette N
authors: NN
authors: Houreld
authors: Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, South Africa. Electronic address: matabs63@yahoo.com.
authors: Sandra M
authors: SM
authors: Ayuk
authors: Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, South Africa. Electronic address: habrahamse@uj.ac.za.
authors: Heidi
authors: H
authors: Abrahamse
mesh_terms: D002460:Cell Line; D003094:Collagen / Q000235:genetics; D016207:Cytokines / Q000235:genetics; D005109:Extracellular Matrix / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects*; D015870:Gene Expression / Q000528:radiation effects*; D020869:Gene Expression Profiling; D006801:Humans; D007249:Inflammation / Q000235:genetics; D007834:Lasers; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction
chemical_list: D016207:Cytokines; D003094:Collagen
keywords: Extracellular matrix; Gene profile; Low level laser therapy