title: C. elegans in high-throughput drug discovery.
abstract: Caenorhabditis elegans has been proven to be a useful model organism for investigating molecular and cellular aspects of numerous human diseases. More recently, investigators have explored the use of this organism as a tool for drug discovery. Although earlier drug screens were labor-intensive and low in throughput, recent advances in high-throughput liquid workflows, imaging platforms and data analysis software have made C. elegans a viable option for automated high-throughput drug screens. This review will outline the evolution of C. elegans-based drug screening, discuss the inherent challenges of using C. elegans, and highlight recent technological advances that have paved the way for future drug screens.
authors: Department of Pediatrics, Cell Biology and Physiology, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh of UPMC, 4401 Penn Avenue, Pittsburgh, PA 15224,USA.
authors: Linda P
authors: LP
authors: O'Reilly
authors: Department of Pediatrics, Cell Biology and Physiology, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh of UPMC, 4401 Penn Avenue, Pittsburgh, PA 15224,USA.
authors: Cliff J
authors: CJ
authors: Luke
authors: Department of Pediatrics, Cell Biology and Physiology, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh of UPMC, 4401 Penn Avenue, Pittsburgh, PA 15224,USA.
authors: David H
authors: DH
authors: Perlmutter
authors: Department of Pediatrics, Cell Biology and Physiology, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh of UPMC, 4401 Penn Avenue, Pittsburgh, PA 15224,USA.
authors: Gary A
authors: GA
authors: Silverman
authors: Department of Pediatrics, Cell Biology and Physiology, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh of UPMC, 4401 Penn Avenue, Pittsburgh, PA 15224,USA. Electronic address: paksc@upmc.edu.
authors: Stephen C
authors: SC
authors: Pak
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000502:physiology*; D055808:Drug Discovery / Q000379:methods* / Q000639:trends; D057166:High-Throughput Screening Assays / Q000379:methods* / Q000639:trends; D006801:Humans
keywords: Clozapine (PubChem CID: 2818); Drug target identification; Fluphenazine (PubChem CID: 3372); High-throughput screening; Human disease models; Nemadipine-A (PubChem CID: 2856102); Pharmaceuticals; Phenotypic analysis; Small molecules; Whole organism model