title: Biodegradation of triazine herbicide metribuzin by the strain Bacillus sp. N1.
abstract: By enrichment culturing of soil contaminated with metribuzin, a highly efficient metribuzin degrading bacterium, Bacillus sp. N1, was isolated. This strain grows using metribuzin at 5.0% (v/v) as the sole nitrogen source in a liquid medium. Optimal metribuzin degradation occurred at a temperature of 30ºC and at pH 7.0. With an initial concentration of 20 mg L(-1), the degradation rate was 73.5% in 120 h. If the initial concentrations were higher than 50 mg L(-1), the biodegradation rates decreased as the metribuzin concentrations increased. When the concentration was 100 mg L(-1), the degradation rate was only 45%. Degradation followed the pesticide degradation kinetic equation at initial concentrations between 5 mg L(-1) and 50 mg L(-1). When the metribuzin contaminated soil was mixed with strain N1 (with the concentration of metribuzin being 20 mg L(-1) and the inoculation rate of 10(11) g(-1) dry soil), the degradation rate of the metribuzin was 66.4% in 30 days, while the degradation rate of metribuzin was only 19.4% in the control soil without the strain N1. These results indicate that the strain N1 can significantly increase the degradation rate of metribuzin in contaminated soil.
authors: a College of Resource and Environment , Jilin Agricultural University , Changchun , People's Republic of China.
authors: Hao
authors: H
authors: Zhang
authors: Yubin
authors: Y
authors: Zhang
authors: Zhiguang
authors: Z
authors: Hou
authors: Xian
authors: X
authors: Wu
authors: Henan
authors: H
authors: Gao
authors: Fengjie
authors: F
authors: Sun
authors: Hongyu
authors: H
authors: Pan
mesh_terms: D001407:Bacillus / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002849:Chromatography, Gas; D006540:Herbicides / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013696:Temperature; D014227:Triazines / Q000378:metabolism*
chemical_list: D006540:Herbicides; D012336:RNA, Ribosomal, 16S; D012987:Soil; D012989:Soil Pollutants; D014227:Triazines; C009235:metribuzin