title: Dopamine disorganizes the rhythm of protein synthesis disrupting self-organization of hepatocytes in vitro.
abstract: We studied dense 24-hour cultures of rat hepatocytes in serum-free medium on collagen-coated slides. As before, a circahoralian rhythm of protein synthesis was observed in control cultures in a fresh medium. No rhythm was found after addition of 1-10 μM dopamine to the medium containing such cultures. The rhythm was observed after addition of 0.3 μM ganglioside to pretreated-dopamine cultures. Dopamine is likely to influence the conditioning of intercellular medium with gangliosides. Deficit of this endogenous synchronizing factor in the intercellular medium blocks self-organization of the protein synthesis rhythm. Thus, in contrast to previously studied norepinephrine and serotonin, as well as gangliosides, which organized the population rhythm of protein synthesis, dopamine disorganized the rhythm, impairing direct intercellular interactions.
authors: N. K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences; N. I. Pirogov Russian National Research Medical University, Moscow, Russia. brodsky.idb@bk.ru.
authors: V Y
authors: VY
authors: Brodskii
authors: T K
authors: TK
authors: Dubovaya
authors: N D
authors: ND
authors: Zvezdina
authors: D S
authors: DS
authors: Konchenko
authors: L A
authors: LA
authors: Mal'chenko
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004298:Dopamine / Q000494:pharmacology*; D015259:Dopamine Agents / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D008297:Male; D014176:Protein Biosynthesis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D015259:Dopamine Agents; D004298:Dopamine