title: Transcriptome analysis identifies regulators of hematopoietic stem and progenitor cells.
abstract: Hematopoietic stem cells (HSCs) maintain blood homeostasis and are the functional units of bone marrow transplantation. To improve the molecular understanding of HSCs and their proximal progenitors, we performed transcriptome analysis within the context of the ImmGen Consortium data set. Gene sets that define steady-state and mobilized HSCs, as well as hematopoietic stem and progenitor cells (HSPCs), were determined. Genes involved in transcriptional regulation, including a group of putative transcriptional repressors, were identified in multipotent progenitors and HSCs. Proximal promoter analyses combined with ImmGen module analysis identified candidate regulators of HSCs. Enforced expression of one predicted regulator, Hlf, in diverse HSPC subsets led to extensive self-renewal activity ex vivo. These analyses reveal unique insights into the mechanisms that control the core properties of HSPCs.
authors: Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA 02115, USA ; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA ; The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel.
authors: Roi
authors: R
authors: Gazit
authors: Brian S
authors: BS
authors: Garrison
authors: Tata Nageswara
authors: TN
authors: Rao
authors: Tal
authors: T
authors: Shay
authors: James
authors: J
authors: Costello
authors: Jeff
authors: J
authors: Ericson
authors: Francis
authors: F
authors: Kim
authors: James J
authors: JJ
authors: Collins
authors: Aviv
authors: A
authors: Regev
authors: Amy J
authors: AJ
authors: Wagers
authors: Derrick J
authors: DJ
authors: Rossi
mesh_terms: D016026:Bone Marrow Transplantation; D002454:Cell Differentiation; D019070:Cell Lineage; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D006412:Hematopoietic Stem Cells; D011401:Promoter Regions, Genetic; D013234:Stem Cells