title: [Drug dynamics research of salvia miltiorrhiza in rabbit plasma and aqueous humor after intravenous injection].
abstract: OBJECTIVE
  To assess the permeability of Danshensu at blood-ocular barrier and its characteristics of pharmacokinetics by respectively measuring the concentrations of Danshensu in blood plasma and aqueous humor of the rabbit with the high performance liquid chromatography(HPLC).
  
  
  METHODS
  It was an experimental study. Seventy-two white rabbits were split into three groups: control group (6 rabbits), plasma group (6 rabbits) and aqueous humor group (60 rabbits). After 0.85% salt water (control group)or salvia miltiorrhiza (1.0 g/kg) (plasma and aqueous group) was injected into the vein of auris-edge. Samples of blood and aqueous humor were obtained for analysis . The analytical column was a BDS C18 stainless steed column(5 µm, 4.6 mm× 250 mm); Precolumn:YWGC18; the mobile phase consists of acetonitrile and 0.01 mol/L KH2PO4 (8:92, adjusted to pH = 2.8 with phosphoric acid). The UV detector was set at 279 nm; the flow rate was 1.0 ml/min;and the column temperature was ordinary temperature.
  
  
  RESULTS
  The plasma concentration-time curves of Salvia miltiorrhiza fitted three-compartment model. t1/2 β (elimination half time): 5.661 min; Cmax (peak concentration): 727.29 mg/L; Tmax(peak time ): 0 min. The aqueous humor concentration-time curves fitted two-compartment model. t1/2 β: 147.663 min; Cmax: 38.62 mg/L; Tmax: 25 min.
  
  
  CONCLUSIONS
  HPLC is a sensitive, specific and accurate method that can be used in pharmacokinetics research on ocular tissue of rabbit for Danshensu, Salvia miltiorrhiza that is dissolvable in water can pass through the blood-ocular barrier after intravenous injection with a relatively stable concentration of danshensu in aqueous humor resulting from a slow rate of removal.
authors: Department of Ophthalmology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors: Li-qiong
authors: LQ
authors: Zhang
authors: Department of Ophthalmology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors: Yong-bin
authors: YB
authors: Yu
authors: Department of Ophthalmology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors: Ming-sheng
authors: MS
authors: Li
authors: Department of Ophthalmology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors: Hui-ping
authors: HP
authors: Qi
authors: Department of Ophthalmology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China. Email: zhanglq600@sina.com.
authors: Hao
authors: H
authors: Cui
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D007275:Injections, Intravenous; D007773:Lactates / Q000097:blood / Q000493:pharmacokinetics*; D011817:Rabbits; D027561:Salvia miltiorrhiza / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D007773:Lactates; C035055:3,4-dihydroxyphenyllactic acid