title: Streptavidin sensor and its sensing mechanism based on water-soluble fluorescence conjugated polymer.
abstract: Fluorescence quenching effect of water-soluble anionic conjugated polymer (CP) (poly[5-methoxy-2-(3-sulfopoxy)-1,4-phenylenevinylene] (MPS-PPV)) by [Re(N-N)(CO)3(py-CH2-NH-biotin)](PF6) [N-N=2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline; py-CH2-NH-biotin=N-[(4-pyridyl) methyl] biotinamide] (Re-Biotin) and fluorescence recovery in the presence of streptavidin (or avidin) were investigated using Re-Biotin as quencher tether ligand (QTL) probe. Meanwhile, the mechanisms of fluorescence quenching and recovery were discussed to provide new thoughts to design biosensor based on water-soluble CPs. The results indicate that the sensing mechanisms of streptavidin sensor or avidin sensor, using Re-Biotin as QTL probe, are the same and stable, whether in non-buffer system (aqueous solution) or different buffer systems [0.01 mol·L(-1) phosphate buffered solution (pH=7.4), 0.1 mol·L(-1) ammonium carbonate buffered solution (pH=8.9)]. There exists specific interactions between streptavidin (or avidin) and biotin of Re-Biotin. Fluorescence quenching and recovery processes of MPS-PPV are reversible. Mechanisms of Re-Biotin quenching MPS-PPV fluorescence can be interpreted as strong electrostatic interactions and charge transferences between Re-Biotin and MPS-PPV. Fluorescence recovery mechanisms of Re-Biotin-MPS-PPV system can be interpreted as specific interactions between streptavidin (or avidin) and biotin of Re-Biotin making Re-Biotin far away from MPS-PPV. Avidin or strptavidin as re-Biotin probe can not only be quantitatively determinated, but also be identified.
authors: College of Chemistry and Chemical Engineering, Hubei University of Technology, Wuhan 430068, China; Key Laboratory of Pollutant Analysis and Reclamation Technology of Hubei, Hubei Normal University, Huangshi 435002, China; College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China. Electronic address: chenyg1965@163.com.
authors: Yanguo
authors: Y
authors: Chen
authors: College of Chemistry and Chemical Engineering, Hubei University of Technology, Wuhan 430068, China.
authors: Peng
authors: P
authors: Hong
authors: College of Chemistry and Chemical Engineering, Hubei University of Technology, Wuhan 430068, China.
authors: Baoming
authors: B
authors: Xu
authors: College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors: Zhike
authors: Z
authors: He
authors: College of Chemistry and Chemical Engineering, Hubei University of Technology, Wuhan 430068, China.
authors: Baohan
authors: B
authors: Zhou
mesh_terms: D001360:Avidin / Q000032:analysis; D001710:Biotin / Q000031:analogs & derivatives* / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D010618:Phenanthrolines / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry; D012995:Solubility; D013050:Spectrometry, Fluorescence; D019809:Streptavidin / Q000032:analysis*; D014867:Water / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D010618:Phenanthrolines; C506109:Poly(2,5-methoxy-propyloxy sulfonate phenylene vinylene); D011145:Polyvinyls; D011721:Pyrenes; C560741:biotinyl-1(1-pyrene)methylamide; D014867:Water; D001360:Avidin; C093086:biotinamide; D001710:Biotin; D019809:Streptavidin; C002478:bathocuproine
keywords: QTL probe; Sensors; Streptavidin; Water-soluble fluorescence conjugated polymer