title: Silencing Dicer expression enhances cellular proliferative and invasive capacities in human tongue squamous cell carcinoma.
abstract: microRNAs (miRNAs) are aberrantly expressed in cancer. An enzyme essential for miRNA processing is Dicer, whose expression is deregulated in diverse types of cancer and correlates with tumor progression. However, whether the regulation of Dicer expression affects tongue squamous cell carcinoma is unknown. In the present study, we investigated how silencing the expression of Dicer alters cell proliferation, cell cycle patterns, and cell migration and invasion in the Tca-8113 tongue squamous cell carcinoma cell line. Dicer expression levels were determined using quantitative PCR and western blot analysis in normal oral gingival epithelial cells and in two tongue squamous cell carcinoma lines, Tca-8113 and UM-1. Tca-8113 cells were transfected with Dicer siRNA or a negative control siRNA. Cell proliferation was determined using the MTT assay and the cell cycle was examined using flow cytometry. Cell migration and invasion changes were evaluated using wound-healing, adherence and Transwell assays. Dicer was expressed at lower levels in the tongue squamous cell carcinoma cell lines Tca-8113 and UM-1 compared to normal gingival epithelial cells, and less Dicer was expressed in UM-1 cells compared to Tca-8113 cells. Notably, Tca-8113 cells transfected with Dicer siRNA had significantly higher proliferative and invasive abilities than cells transfected with the negative control siRNA or non-transfected cells. Silencing Dicer may promote the progression of tongue squamous cell carcinoma. Dicer could serve a promising biomarker and a potential therapeutic target for tongue squamous cell carcinoma.
authors: Stomatological Hospital of Guangdong Province Affiliated to Southern Medical University, Guangzhou, Guangdong 510280, P.R. China.
authors: Shuguang
authors: S
authors: Zeng
authors: Stomatological Hospital of Guangdong Province Affiliated to Southern Medical University, Guangzhou, Guangdong 510280, P.R. China.
authors: Jing
authors: J
authors: Yang
authors: Stomatological Hospital of Guangdong Province Affiliated to Southern Medical University, Guangzhou, Guangdong 510280, P.R. China.
authors: Jianjiang
authors: J
authors: Zhao
authors: Department of Molecular Biology, Guangzhou Medical College, Guangzhou, Guangdong 510120, P.R. China.
authors: Qicai
authors: Q
authors: Liu
authors: Stomatological Hospital of Guangdong Province Affiliated to Southern Medical University, Guangzhou, Guangdong 510280, P.R. China.
authors: Mingdeng
authors: M
authors: Rong
authors: Stomatological Hospital of Guangdong Province Affiliated to Southern Medical University, Guangzhou, Guangdong 510280, P.R. China.
authors: Zehong
authors: Z
authors: Guo
authors: Stomatological Hospital of Guangdong Province Affiliated to Southern Medical University, Guangzhou, Guangdong 510280, P.R. China.
authors: Wenfeng
authors: W
authors: Gao
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002448:Cell Adhesion / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D053487:DEAD-box RNA Helicases / Q000096:biosynthesis / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D005881:Gingiva / Q000166:cytology; D006801:Humans; D035683:MicroRNAs / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering; D043244:Ribonuclease III / Q000096:biosynthesis / Q000235:genetics*; D014062:Tongue Neoplasms / Q000235:genetics* / Q000473:pathology; D025521:Tumor Suppressor Proteins / Q000235:genetics; D014945:Wound Healing / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D034741:RNA, Small Interfering; D025521:Tumor Suppressor Proteins; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases