title: MicroRNA-34a regulates high glucose-induced apoptosis in H9c2 cardiomyocytes.
abstract: Hyperglycemia is an important initiator of cardiovascular disease, contributing to the development of cardiomyocyte death and diabetic complications. The purpose of the present study was to investigate whether high glucose state could induce apoptosis of rat cardiomyocyte cell line H9c2 through microRNA-mediated Bcl-2 signaling pathway. The expression of miR-34a and Bcl-2 mRNA was detected by using real-time PCR. Western blotting was used to examine the changes in apoptosis-associated protein Bcl-2. Apoptosis of H9c2 cells was tested by using flow cytometry. The results showed that the expression of miR-34a was significantly elevated and that of Bcl-2 was strongly reduced, and apoptosis of cardiomyocytes was apparently increased in the high-glucose-treated H9c2 cells as compared with normal-glucose-treated controls. In addition, we identified Bcl-2 gene was the target of miR-34a. miR-34a mimics reduced the expression of Bcl-2 and increased glucose-induced apoptosis, but miR-34a inhibitor acted as the opposite mediator. Our data demonstrate that miR-34a contributes to high glucose-induced decreases in Bcl-2 expression and subsequent cardiomyocyte apoptosis.
authors: Department of Cardiovascular Diseases, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China. cedilanid@163.com.
authors: Fang
authors: F
authors: Zhao
authors: Department of Oral Radiology, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.
authors: Bo
authors: B
authors: Li
authors: Department of Cardiovascular Diseases, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.
authors: Yin-Zhi
authors: YZ
authors: Wei
authors: Department of Cardiovascular Diseases, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.
authors: Bin
authors: B
authors: Zhou
authors: Department of Cardiovascular Diseases, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.
authors: Han
authors: H
authors: Wang
authors: Department of Cardiovascular Diseases, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.
authors: Ming
authors: M
authors: Chen
authors: Department of Cardiovascular Diseases, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.
authors: Xue-Dong
authors: XD
authors: Gan
authors: Department of Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors: Zhao-Hui
authors: ZH
authors: Wang
authors: Department of Cardiovascular Diseases, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.
authors: Shi-Xi
authors: SX
authors: Xiong
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D005947:Glucose / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D051381:Rats
chemical_list: C560944:MIRN34 microRNA, rat; D035683:MicroRNAs; D005947:Glucose