title: Improved propionic acid production from glycerol with metabolically engineered Propionibacterium jensenii by integrating fed-batch culture with a pH-shift control strategy.
abstract: Propionic acid (PA) production with metabolically engineered Propionibacterium jensenii (pZGX04-gldA) was improved by integrating fed-batch culture with a two-stage pH control strategy in a 3-L fermenter. The following two-stage pH control strategy was used: the pH was controlled at 5.9 for 0-36 h and shifted to 6.5 after 36 h. The PA titer was increased to 21.43 g/L. On the basis of pH control, the influence of fed-batch culture on PA production was further investigated and the maximum PA production (34.62 g/L) was obtained when glycerol was fed at a constant rate of 3.33 mL/h from 60 to 132 h with an initial glycerol concentration of 25 g/L. Crude glycerol was then used to produce PA using the optimized strategies, and maximal PA production reached 37.26 g/L. The strategies may be useful for the production of PA by other propionibacteria species.
authors: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Wuxi 214122, China.
authors: Xin
authors: X
authors: Zhuge
authors: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Wuxi 214122, China.
authors: Long
authors: L
authors: Liu
authors: School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta 30332, USA.
authors: Hyun-dong
authors: HD
authors: Shin
authors: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Wuxi 214122, China. Electronic address: lijianghua@jiangnan.edu.cn.
authors: Jianghua
authors: J
authors: Li
authors: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Wuxi 214122, China. Electronic address: gcdu@jiangnan.edu.cn.
authors: Guocheng
authors: G
authors: Du
authors: Synergetic Innovation Center of Food Safety and Nutrition, Wuxi 214122, China; National Engineering of Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China.
authors: Jian
authors: J
authors: Chen
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods*; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D060847:Metabolic Engineering; D011422:Propionates / Q000378:metabolism*; D011424:Propionibacterium / Q000254:growth & development / Q000378:metabolism*
chemical_list: D011422:Propionates; C029658:propionic acid; D005990:Glycerol
keywords: Glycerol; Metabolic engineering; Propionibacterium jensenii; Propionic acid