title: Genetic variability of the neogregarine Apicystis bombi, an etiological agent of an emergent bumblebee disease.
abstract: The worldwide spread of diseases is considered a major threat to biodiversity and a possible driver of the decline of pollinator populations, particularly when novel species or strains of parasites emerge. Previous studies have suggested that populations of introduced European honeybee (Apis mellifera) and bumblebee species (Bombus terrestris and Bombus ruderatus) in Argentina share the neogregarine parasite Apicystis bombi with the native bumblebee (Bombus dahlbomii). In this study we investigated whether A. bombi is acting as an emergent parasite in the non-native populations. Specifically, we asked whether A. bombi, recently identified in Argentina, was introduced by European, non-native bees. Using ITS1 and ITS2 to assess the parasite's intraspecific genetic variation in bees from Argentina and Europe, we found a largely unstructured parasite population, with only 15% of the genetic variation being explained by geographic location. The most abundant haplotype in Argentina (found in all 9 specimens of non-native species) was identical to the most abundant haplotype in Europe (found in 6 out of 8 specimens). Similarly, there was no evidence of structuring by host species, with this factor explaining only 17% of the genetic variation. Interestingly, parasites in native Bombus ephippiatus from Mexico were genetically distant from the Argentine and European samples, suggesting that sufficient variability does exist in the ITS region to identify continent-level genetic structure in the parasite. Thus, the data suggest that A. bombi from Argentina and Europe share a common, relatively recent origin. Although our data did not provide information on the direction of transfer, the absence of genetic structure across space and host species suggests that A. bombi may be acting as an emergent infectious disease across bee taxa and continents.
authors: Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
authors: Jafar
authors: J
authors: Maharramov
authors: Ivan
authors: I
authors: Meeus
authors: Kevin
authors: K
authors: Maebe
authors: Marina
authors: M
authors: Arbetman
authors: Carolina
authors: C
authors: Morales
authors: Peter
authors: P
authors: Graystock
authors: William O H
authors: WO
authors: Hughes
authors: Santiago
authors: S
authors: Plischuk
authors: Carlos E
authors: CE
authors: Lange
authors: Dirk C
authors: DC
authors: de Graaf
authors: Nelson
authors: N
authors: Zapata
authors: Jose Javier Perez
authors: JJ
authors: de la Rosa
authors: Tomás E
authors: TE
authors: Murray
authors: Mark J F
authors: MJ
authors: Brown
authors: Guy
authors: G
authors: Smagghe
mesh_terms: D000818:Animals; D016782:Apicomplexa / Q000235:genetics*; D001118:Argentina; D001516:Bees / Q000469:parasitology*; D005075:Biological Evolution; D005060:Europe; D014644:Genetic Variation; D005843:Geography; D006239:Haplotypes / Q000235:genetics; D008969:Molecular Sequence Data; D013045:Species Specificity