title: Allicin attenuates inflammation and suppresses HLA-B27 protein expression in ankylosing spondylitis mice.
abstract: Here we aimed to determine the therapeutic effect of allicin on ankylosing spondylitis (AS) and explore the mechanism(s) of action. AS mouse model was constructed by transferring the HLA-B2704 gene into Kunming mice and verified by RT-PCR and CT imaging. Verified AS mice were randomly divided into model group (n = 6) and allicin-treated groups (50, 100, and 200 mg/kg, resp., n = 6, p.o., for 2 months). Wild type mice were used as control (n = 6). The levels of AS-related inflammatory factors were measured by ELISA. mRNA and protein expressions of HLA-B27 were checked by RT-PCR and western blotting. As the results, the mouse model of AS was successfully established, and high-dose allicin could markedly alleviate spine inflammatory injury possibly via reducing the secretion of the inflammatory factors (IL-6, IL-8, and TNF- α ) sharply in AS mice. Moreover, allicin significantly inhibited HLA-B27 protein translation but failed to suppress HLA-B27 gene transcription in AS mice, indicating a posttranscriptional mechanism of this modulation. In conclusion, allicin has potential to be used for AS treatment as an anti-inflammatory nutraceutical.
authors: Department of Orthopaedics, Shanghai Tenth People's Hospital Affiliated to TongJi University, Shanghai 20072, China ; Department of Orthopaedics, The Second Affiliated Hospital of the Second Military Medical University, No. 415 Fengyang Road, Huangpu District, Shanghai 200003, China.
authors: Xin
authors: X
authors: Gu
authors: Haishan
authors: H
authors: Wu
authors: Peiliang
authors: P
authors: Fu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004220:Disulfides; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D015796:HLA-B27 Antigen / Q000096:biosynthesis* / Q000276:immunology; D006801:Humans; D007249:Inflammation / Q000188:drug therapy* / Q000473:pathology; D015850:Interleukin-6 / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis; D051379:Mice; D008822:Mice, Transgenic; D013167:Spondylitis, Ankylosing / Q000188:drug therapy* / Q000473:pathology; D013441:Sulfinic Acids / Q000008:administration & dosage*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D004220:Disulfides; D015796:HLA-B27 Antigen; D015850:Interleukin-6; D016209:Interleukin-8; D013441:Sulfinic Acids; D014409:Tumor Necrosis Factor-alpha; C006452:allicin