title: Acute effects of aqueous leaf extract of Aspilia africana C.D. Adams on some haematological parameters in rats.
abstract: Several medicinal plants have been documented for their haematological effects either at low or high concentration but very little is known about Aspilia africana. The aim of the study was to investigate the acute effects of aqueous leaf extract of Aspilia africana at different concentrations on some haematological parameters in rats. Following 14 days of oral administration of aqueous extract of A. africana, Haematocrit (HCT), Haemoglobin concentration (HB), Mean Cell Haemoglobin Concentration (MCHC), Red Blood Cell Count (RBC Count), Total White Blood Cell Count (Total WBC Count), Absolute Neutrophils count (NEUT#), Absolute Lymphocytes count (LYM#), Absolute Eosinophils Count (EOSIN#) and Absolute Monocytes (MONO#) were evaluated in twenty (20) male Wistar albino rats. The rats weighed 174 ± 20 g, and were randomly assigned into 4 groups viz: Group 1, Control; Group 2, 250 mg/Kg/d aqueous extract; Group 3, 500 mg/Kg/d aqueous extract; and Group 4, 750 mg/Kg/d aqueous extract. HCT, HB, MCHC, RBC Count, Total WBC Count, NEUT#, LYM#, EOSIN# and MONO# were significantly increased (P<0.001) in 500 mg/Kg/d of A. africana extract (61.13 ± 1.65%, 13.5 ± 1.29 g/dl, 23.33 ± 0.0.02 g/dl, 3.68 ± 0.02 X 10(12)Cells/l, 2.33 ± 0.02 X 10(9)Cells/l, 1.32 ± 0.04 X 10(9)Cells/l, 1.43 ± 0.05 X 10(9)Cells/l, 0.47 ± 0.02 X 10(9)Cells/l and 0.47 ± 0.04 X 10(9)Cells/l, respectively) when compared to the Control (51.13 ± 0.85%, 9.56 ± 0.43 g/dl, 19.22 ± 0.19 g/dl, 2.69 ± 0.01 X 10(12)Cells/l, 1.79 ± 0.01 X 10(9)Cells/l, 0.80 ± 0.00 X 10(9)Cells/l, 0.83 ± 0.00 X 10(9)Cells/l, 0.18 ± 0.00 X 10(9)Cells/l and 0.24 ± 0.00 X 10(9)Cells/l, respectively) which received no extract at all. The 500 mg/Kg of A. africana extract proved to be the most effective, while the 750 mg/Kg proved to be the least effective in comparison with the control. The results of this study further strengthened the earlier works on the medicinal benefits of Aspilia africana and its virtue as a good pharmacological source of haematopoiesis.
authors: Department of Physiology, School of Basic Medical Sciences Igbinedion University, Okada, Edo State, Nigeria.
authors: Kazeem Olasunkanmi
authors: KO
authors: Ajeigbe
authors: Seyi Samson
authors: SS
authors: Enitan
authors: Dayo Rotimi
authors: DR
authors: Omotoso
authors: Olayemi Olutobi
authors: OO
authors: Oladokun
mesh_terms: D000818:Animals; D019659:Asteraceae; D001772:Blood Cell Count; D004909:Erythrocyte Indices / Q000187:drug effects*; D004912:Erythrocytes / Q000378:metabolism*; D006400:Hematocrit; D006410:Hematopoiesis / Q000187:drug effects*; D006454:Hemoglobins / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006454:Hemoglobins; D010936:Plant Extracts
keywords: Aspilia Africana; haematology; rats