title: Subcellular localization of glutelin-2 in maize (Zea mays L.) endosperm.
abstract: Accumulation of the 28 KD protein of the glutelin-(G2) fraction was followed in developing maize endosperm, using sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) and peak integration of scanned gels. 28 KD glutelin-2 could already be observed from 15 days after pollination and its accumulates reached a plateau during the second half of the development period. The process of biosynthesis of 28 KD glutelin-2 and zeins occurs in a parallel way. Subcellular fractions obtained from linear sucrose gradient centrifugation of developing maize endosperms were analyzed by SDS-PAGE and immunoblotting using a serum reacting against glutelin-2 and 14 KD Z2. Glutelin-2 was found to be present in the protein bodies when subcellular fractionation was carried out without dithiothreitol (DTT). The presence of a reducing agent causes the elution of glutelin-2 from protein bodies. Immunocytochemical labelling using the protein A-colloidal gold technique in protein bodies incubated with anti-G2 IgG revealed that G2 is located mainly in the periphery of protein bodies. These results are interpreted as indicating a structural role for glutelins in protein bodies.
authors: Institut de Biologia de Barcelona, Unitat de Biofisica i Biologia Molecular, CSIC, Jorge Girona Salgado, 18-26, Barcelona-34, Spain.
authors: M D
authors: MD
authors: Ludevid
authors: M
authors: M
authors: Torrent
authors: J A
authors: JA
authors: Martinez-Izquierdo
authors: P
authors: P
authors: Puigdomenech
authors: J
authors: J
authors: Palau