title: Scavenging systems for reactive carbonyls in the cyanobacterium Synechocystis sp. PCC 6803.
abstract: To elucidate the scavenging systems of sugar- and lipid-derived reactive carbonyls (RCs) in the cyanobacterium Synechocystis sp. PCC 6803 (S. 6803), we selected proteins from S. 6803 based on amino-acid (AA) sequence similarities with proteins from Arabidopsis thaliana, and characterized the properties of the GST-fusion proteins expressed. Slr0942 catalyzed the aldo-keto reductase (AKR) reaction scavenging mainly sugar-derived RCs, methylglyoxal (MG). Slr1192 is the medium-chain dehydrogenase/redutase (MDR). It catalyzed the AKR reaction scavenging several lipid-derived RCs, acrolein, propionaldehyde, and crotonaldehyde. Slr0315 is a short-chain dehydrogenase/redutase (SDR), and it catalyzed only the reduction of MG in the AKR reaction. Slr0381 catalyzed the conversion of hemithioacetal to S-lactoylglutahione (SLG) in the glyoxalase (GLX) 1 reaction. Sll1019 catalyzed the conversion of SLG to glutathione and lactate in the GLX2 reaction. GLX1 and GLX2 compose the glyoxalase system, which scavenges MG. These enzymes contribute to scavenging sugar- and lipid-derived RCs as scavenging systems.
authors: Department of Biological and Environmental Sciences, Faculty of Agriculture, Graduate School of Agricultural Science, Kobe University.
authors: Ginga
authors: G
authors: Shimakawa
authors: Mayumi
authors: M
authors: Suzuki
authors: Eriko
authors: E
authors: Yamamoto
authors: Akiko
authors: A
authors: Nishi
authors: Ryota
authors: R
authors: Saito
authors: Katsuhiko
authors: K
authors: Sakamoto
authors: Hiroshi
authors: H
authors: Yamamoto
authors: Amane
authors: A
authors: Makino
authors: Chikahiro
authors: C
authors: Miyake
mesh_terms: D000449:Aldehyde Reductase / Q000378:metabolism; D000447:Aldehydes / Q000378:metabolism*; D000074408:Aldo-Keto Reductases; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D007659:Ketones / Q000378:metabolism*; D008969:Molecular Sequence Data; D046939:Synechocystis / Q000378:metabolism*
chemical_list: D000447:Aldehydes; D001426:Bacterial Proteins; D007659:Ketones; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase