title: A characterization study of resveratrol/sulfobutyl ether-β-cyclodextrin inclusion complex and in vitro anticancer activity.
abstract: A resveratrol/sulfobutylether-β-cyclodextrin inclusion complex was prepared using the freeze-drying method and characterized in solution through UV-vis spectroscopy, solubility phase studies and Job's plot methods. At the solid state it was characterized using the FTIR-ATR technique. Sulfobutylether-β-cyclodextrin has a high affinity for the drug, and forms an inclusion complex with a 1:1 molar ratio both in solution and as a solid sample. It also has a high stability constant (Kc, 10,114 M(-1)). Complexation strongly increases the water solubility of resveratrol (from 0.03 mg/ml to 1.1 mg/ml, at 25 °C) and positively influences its in vitro anticancer activity which was observed on a human breast cancer cell line (MCF-7). In solid phase, FTIR-ATR revealed itself as being a useful technique in elucidating the complexation mechanism, which it did by emphasizing the functional groups involved in the activation of non-covalent "host-guest" interactions.
authors: Dipartimento di Fisica e di Scienze della Terra, Università degli Studi di Messina, Viale Ferdinando Stagno D'Alcontres 31, I-98166 Messina, Italy.
authors: Valentina
authors: V
authors: Venuti
authors: Dipartimento di Scienze del Farmaco e Prodotti per la Salute, Università degli Studi di Messina, V.le Annunziata, I-98168 Messina, Italy.
authors: Carmela
authors: C
authors: Cannavà
authors: Dipartimento di Scienze della Salute, Università Magna Graecia di Catanzaro, Viale Europa, I-88100 Catanzaro, Italy.
authors: Maria Chiara
authors: MC
authors: Cristiano
authors: Dipartimento di Scienze della Salute, Università Magna Graecia di Catanzaro, Viale Europa, I-88100 Catanzaro, Italy.
authors: Massimo
authors: M
authors: Fresta
authors: Dipartimento di Fisica e di Scienze della Terra, Università degli Studi di Messina, Viale Ferdinando Stagno D'Alcontres 31, I-98166 Messina, Italy.
authors: Domenico
authors: D
authors: Majolino
authors: Dipartimento di Scienze della Salute, Università Magna Graecia di Catanzaro, Viale Europa, I-88100 Catanzaro, Italy.
authors: Donatella
authors: D
authors: Paolino
authors: Dipartimento di Scienze del Farmaco e Prodotti per la Salute, Università degli Studi di Messina, V.le Annunziata, I-98168 Messina, Italy.
authors: Rosanna
authors: R
authors: Stancanelli
authors: Dipartimento di Scienze del Farmaco e Prodotti per la Salute, Università degli Studi di Messina, V.le Annunziata, I-98168 Messina, Italy.
authors: Silvana
authors: S
authors: Tommasini
authors: Dipartimento di Scienze del Farmaco e Prodotti per la Salute, Università degli Studi di Messina, V.le Annunziata, I-98168 Messina, Italy. Electronic address: caventura@unime.it.
authors: Cinzia Anna
authors: CA
authors: Ventura
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D006801:Humans; D061986:MCF-7 Cells; D000077185:Resveratrol; D012995:Solubility; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013267:Stilbenes / Q000737:chemistry / Q000494:pharmacology*; D047392:beta-Cyclodextrins / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D012996:Solutions; D013267:Stilbenes; D047392:beta-Cyclodextrins; C093196:SBE4-beta-cyclodextrin; D000077185:Resveratrol
keywords: Anticancer activity; FTIR-ATR; Job's plot; Resveratrol; Sulfobutylether-β-cyclodextrin