title: Nanobioprobe mediated DNA aptamers for explosive detection.
abstract: Specific nucleic acid aptamers using the microtiter plate based modified SELEX method against explosive trinitrotoluene are reported. Efficient partitioning of dsDNA was carried out using streptavidin labeled gold nanoprobes for the selection of specific aptamers. The selected binders having an affinity of ~10(-7) M were used in the newly developed electrochemical aptasensor, exhibiting a detection limit of around 1 ppb for trinitrotoluene.
authors: Priyanka
authors: Munish
authors: M
authors: Shorie
authors: Vijayender
authors: V
authors: Bhalla
authors: Preeti
authors: P
authors: Pathania
authors: C Raman
authors: CR
authors: Suri
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D055664:Electrochemical Techniques; D004566:Electrodes; D053834:Explosive Agents / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D052156:SELEX Aptamer Technique; D019809:Streptavidin / Q000737:chemistry; D014303:Trinitrotoluene / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D053834:Explosive Agents; D014303:Trinitrotoluene; D006046:Gold; D004247:DNA; D019809:Streptavidin