title: Potentiometric sensors doped with biomolecules as a new approach to small molecule/biomolecule binding kinetics analysis.
abstract: The most successful binding kinetics analysis systems at this moment include surface plasmon resonance (SPR), quartz microcrystal balance (QMB) and surface acoustic wave (SAW). Although these are powerful methods, they generally are complex, expensive and require the use of monolayers. Here, we report on potentiometric sensors as an inexpensive and simple alternative to do binding kinetics analysis between small molecules in solution and biomolecules (covalently) attached in a biopolymer sensor coating layer. As an example, dopamine and an anti-dopamine aptamer were used as the small molecule and the biomolecule respectively. Binding between both follows a Langmuir adsorption type model and creates a surface potential. The system operates in Flow Injection Analysis mode (FIA). Besides being an interesting new binding kinetics tool, the approach allows systematic design of potentiometric biosensors (in the present study a dopamine sensor), and gives new insights into the functioning of ion-selective electrodes (ISE's).
authors: University of Antwerp, Chemistry Department, Groenenborgerlaan 171, B-2020 Antwerp, Belgium; University of Antwerp, Centre of Medical Genetics, Universiteitsplein 1, B-2610 Antwerp, Belgium.
authors: D
authors: D
authors: Daems
authors: University of Antwerp, Chemistry Department, Groenenborgerlaan 171, B-2020 Antwerp, Belgium.
authors: K
authors: K
authors: De Wael
authors: University of Antwerp, Biology Department, Groenenborgerlaan 171, B-2020 Antwerp, Belgium.
authors: K
authors: K
authors: Vissenberg
authors: University of Antwerp, Centre of Medical Genetics, Universiteitsplein 1, B-2610 Antwerp, Belgium.
authors: G
authors: G
authors: Van Camp
authors: University of Antwerp, Chemistry Department, Groenenborgerlaan 171, B-2020 Antwerp, Belgium. Electronic address: luc.nagels@ua.ac.be.
authors: L
authors: L
authors: Nagels
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004298:Dopamine / Q000032:analysis*; D015259:Dopamine Agents / Q000032:analysis*; D004867:Equipment Design; D017022:Flow Injection Analysis / Q000295:instrumentation; D007700:Kinetics; D011199:Potentiometry / Q000295:instrumentation*
chemical_list: D052157:Aptamers, Nucleotide; D015259:Dopamine Agents; D004298:Dopamine
keywords: Aptamer; Binding kinetics; Biosensor; Dopamine; Potentiometry