title: Toward T cell protein-protein interaction activity relevant to alopecia areata.
abstract: Development of better therapies for the T cell-mediated autoimmune disease alopecia areata (AA) could be expedited by an improved understanding of the immunologic signals underlying its pathogenesis. To approach this, our group is mounting a new technological and analytical platform, multiplex immunoprecipitation detected by flow cytometry (MIF). MIF is designed to allow analysis of collections of protein-protein interactions that participate in T cell signaling webs. Early experiments suggest that MIF can detect the increased protein-protein interaction network activity that occurs under conditions of T cell antigenic stimulation. Future experiments will focus on application of MIF to T cells isolated from AA or control patient samples, to identify critical T cell signaling complexes associated with the disorder.
authors: Department of Immunology, College of Medicine, Mayo Clinic, Rochester, Minnesota, USA.
authors: Steven C
authors: SC
authors: Neier
authors: Stephen E P
authors: SE
authors: Smith
authors: Tessa R
authors: TR
authors: Davis
authors: Diana
authors: D
authors: Gil
authors: Adam G
authors: AG
authors: Schrum
mesh_terms: D000506:Alopecia Areata / Q000188:drug therapy / Q000276:immunology*; D005434:Flow Cytometry; D005453:Fluorescence; D006801:Humans; D047468:Immunoprecipitation; D008863:Microspheres; D010799:Phycoerythrin; D060066:Protein Interaction Maps; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D010799:Phycoerythrin