title: Efficient production of l-lactic acid using co-feeding strategy based on cane molasses/glucose carbon sources.
abstract: L-Lactic acid is an important platform chemical, which ought to be produced under cost control to meet its huge demand. Cane molasses, a waste from sugar manufacturing processes, is hopeful to be utilized as a cheap carbon source for L-lactic acid fermentation. Considering that cane molasses contains nutrients and hazardous substances, efficient production of L-lactic acid was developed by using a co-feeding strategy based on the utilization of cane molasses/glucose carbon sources. Based on the medium optimization with response surface method, 168.3g/L L-lactic acid was obtained by a Bacillus coagulans strain H-1 after 78h fed-batch fermentation, with a productivity of 2.1g/Lh and a yield of 0.88g/g. Since cane molasses is a feasible carbon source, the co-feeding fermentation might be a promising alternative for the economical production of L-lactic acid.
authors: State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China; School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
authors: Ke
authors: K
authors: Xu
authors: State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China; School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China. Electronic address: pingxu@sjtu.edu.cn.
authors: Ping
authors: P
authors: Xu
mesh_terms: D061249:Batch Cell Culture Techniques; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002244:Carbon / Q000494:pharmacology*; D003470:Culture Media / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D019344:Lactic Acid / Q000096:biosynthesis*; D008965:Molasses; D009584:Nitrogen / Q000191:economics / Q000494:pharmacology; D031786:Saccharum / Q000737:chemistry*; D013997:Time Factors
chemical_list: D003470:Culture Media; D019344:Lactic Acid; D002244:Carbon; D005947:Glucose; D009584:Nitrogen
keywords: Bacillus coagulans; Cane molasses; Co-feeding strategy; l-Lactic acid