title: Effects of cryopreservation on the cell viability, proliferative capacity and neuronal differentiation potential of canine bone marrow stromal cells.
abstract: We investigated the cell viability, proliferative capacity and neuronal differentiation potential of canine bone marrow stromal cells (BMSCs) after cryopreservation. BMSCs were cryopreserved using cryoprotectant solutions with 10% DMSO and 10% FBS (DF group) or without DMSO and FBS (DF-free group); fresh BMSCs were used as a control. The cell viability and proliferative capacity of BMSCs were similar in the DF-free and control groups, while those in the DF group were lower. In all groups, BMSCs differentiated into neuron-like cells that stained positive against neuron markers, and the mRNA expression levels of neuron markers increased after neuronal induction. In conclusion, cryopreservation with DF-free cryoprotectant solution did not diminish the cell viability, proliferative capacity or neuronal differentiation potential of canine BMSCs.
authors: Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors: Kazuya
authors: K
authors: Edamura
authors: Rei
authors: R
authors: Nakano
authors: Kyohei
authors: K
authors: Fujimoto
authors: Kenji
authors: K
authors: Teshima
authors: Kazushi
authors: K
authors: Asano
authors: Shigeo
authors: S
authors: Tanaka
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology*; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D017931:DNA Primers / Q000235:genetics; D004121:Dimethyl Sulfoxide; D004285:Dogs / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000502:physiology*
chemical_list: D015415:Biomarkers; D017931:DNA Primers; D004121:Dimethyl Sulfoxide