title: Development of FRET biosensors for mammalian and plant systems.
abstract: Genetically encoded biosensors are increasingly used in visualising signalling processes in different organisms. Sensors based on green fluorescent protein technology are providing a great opportunity for using Förster resonance energy transfer (FRET) as a tool that allows for monitoring dynamic processes in living cells. The development of these FRET biosensors requires careful selection of fluorophores, substrates and recognition domains. In this review, we will discuss recent developments, strategies to create and optimise FRET biosensors and applications of FRET-based biosensors for use in the two major eukaryotic kingdoms and elaborate on different methods for FRET detection.
authors: Laboratory of Biochemistry and Microspectroscopy Centre, Wageningen University, Wageningen, The Netherlands.
authors: Danny
authors: D
authors: Hamers
authors: Laura
authors: L
authors: van Voorst Vader
authors: Jan Willem
authors: JW
authors: Borst
authors: Joachim
authors: J
authors: Goedhart
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D008322:Mammals; D008856:Microscopy, Fluorescence / Q000379:methods*; D010944:Plants
chemical_list: D005456:Fluorescent Dyes