title: The cannabinoid receptor type 2 as mediator of mesenchymal stromal cell immunosuppressive properties.
abstract: Mesenchymal stromal cells are non-hematopoietic, multipotent progenitor cells producing cytokines, chemokines, and extracellular matrix proteins that support hematopoietic stem cell survival and engraftment, influence immune effector cell development, maturation, and function, and inhibit alloreactive T-cell responses. The immunosuppressive properties of human mesenchymal stromal cells have attracted much attention from immunologists, stem cell biologists and clinicians. Recently, the presence of the endocannabinoid system in hematopoietic and neural stem cells has been demonstrated. Endocannabinoids, mainly acting through the cannabinoid receptor subtype 2, are able to modulate cytokine release and to act as immunosuppressant when added to activated T lymphocytes. In the present study, we have investigated, through a multidisciplinary approach, the involvement of the endocannabinoids in migration, viability and cytokine release of human mesenchymal stromal cells. We show, for the first time, that cultures of human mesenchymal stromal cells express all of the components of the endocannabinoid system, suggesting a potential role for the cannabinoid CB2 receptor as a mediator of anti-inflammatory properties of human mesenchymal stromal cells, as well as of their survival pathways and their capability to home and migrate towards endocannabinoid sources.
authors: Department of Women, Child and General and Specialistic Surgery, Second University of Naples, Naples, Italy ; Department of Onco-Haematology, IRCCS "Bambino Gesù" Children Hospital, Rome, Italy.
authors: Francesca
authors: F
authors: Rossi
authors: Maria Ester
authors: ME
authors: Bernardo
authors: Giulia
authors: G
authors: Bellini
authors: Livio
authors: L
authors: Luongo
authors: Antonella
authors: A
authors: Conforti
authors: Iolanda
authors: I
authors: Manzo
authors: Francesca
authors: F
authors: Guida
authors: Luigia
authors: L
authors: Cristino
authors: Roberta
authors: R
authors: Imperatore
authors: Stefania
authors: S
authors: Petrosino
authors: Bruno
authors: B
authors: Nobili
authors: Vincenzo
authors: V
authors: Di Marzo
authors: Franco
authors: F
authors: Locatelli
authors: Sabatino
authors: S
authors: Maione
mesh_terms: D000328:Adult; D002454:Cell Differentiation; D002465:Cell Movement; D002470:Cell Survival; D016207:Cytokines / Q000378:metabolism; D063388:Endocannabinoids / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D056747:Immunomodulation; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D008875:Middle Aged; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D043884:Receptor, Cannabinoid, CB1 / Q000235:genetics / Q000378:metabolism; D043885:Receptor, Cannabinoid, CB2 / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000378:metabolism; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D055815:Young Adult
chemical_list: D016207:Cytokines; D063388:Endocannabinoids; D018836:Inflammation Mediators; D043884:Receptor, Cannabinoid, CB1; D043885:Receptor, Cannabinoid, CB2; D038762:Ribosomal Protein S6 Kinases, 70-kDa; D058570:TOR Serine-Threonine Kinases; C448901:ribosomal protein S6 kinase, 70kD, polypeptide 1; D019950:Mitogen-Activated Protein Kinase 1