title: C-type lectin binds to β-integrin to promote hemocytic phagocytosis in an invertebrate.
abstract: Phagocytosis is a conserved cellular response among metazoans. Opsonins are some molecules that label targets to increase their susceptibility to phagocytosis. Opsonins are usually captured by receptors on the surface of phagocytes. Our previous study found the C-type lectin FcLec4 from Chinese white shrimp Fenneropenaeus chinensis might function as an opsonin to facilitate bacterial clearance. In the present study we purified the native FcLec4 protein and confirmed its opsonic activity in the near relation, kuruma shrimp Marsupenaeus japonicus. The possible receptor of FcLec4 was identified as β-integrin by panning a T7 phage display library of shrimp hemocytes and then confirmed by co-immunoprecipitation assay. We further proved that the interaction between FcLec4 and β-integrin did not rely on the carbohydrate recognition domain but on the N terminus of FcLec4. In addition, inhibition of FcLec4 expression using RNAi delayed bacterial clearance, and β-integrin knockdown suppressed the opsonic activity of FcLec4. This study is the first to show the direct interaction between an opsonin and its receptor in crustaceans. Our study provides new insights into invertebrate phagocytosis and the functions of C-type lectins.
authors: From the Key Laboratory of Plant Cell Engineering and Germplasm Innovation of Ministry of Education/Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong 250100, China.
authors: Xian-Wei
authors: XW
authors: Wang
authors: Xiao-Fan
authors: XF
authors: Zhao
authors: Jin-Xing
authors: JX
authors: Wang
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics / Q000378:metabolism*; D006434:Hemocytes / Q000166:cytology / Q000378:metabolism*; D039641:Integrin beta Chains / Q000235:genetics / Q000378:metabolism*; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism*; D033561:Penaeidae / Q000235:genetics / Q000378:metabolism*; D019151:Peptide Library; D010587:Phagocytosis / Q000502:physiology*
chemical_list: D060829:Arthropod Proteins; D039641:Integrin beta Chains; D037181:Lectins, C-Type; D019151:Peptide Library
keywords: C-type Lectin; Integrin; Invertebrates; Opsonin; Phagocytosis; Protein-Protein Interactions; RNA Interference (RNAi)