title: A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells.
abstract: We compared different techniques for measuring gut HIV reservoirs and assessed for HIV in non-CD4 T cells. HIV DNA levels were similar when measured from rectal biopsies and isolated rectal cells, while HIV RNA tended to be higher in rectal cells. HIV DNA levels in total rectal cells were greater than those predicted from levels in sorted CD4 T cells, suggesting a reservoir in non-CD4 T cells, and HIV DNA was detected in sorted myeloid cells (7/7 subjects).
authors: aSan Francisco Veterans Affairs Medical Center bUniversity of California, San Francisco (UCSF), California, USA.
authors: Steven A
authors: SA
authors: Yukl
authors: Elizabeth
authors: E
authors: Sinclair
authors: Ma
authors: M
authors: Somsouk
authors: Peter W
authors: PW
authors: Hunt
authors: Lorrie
authors: L
authors: Epling
authors: Maudi
authors: M
authors: Killian
authors: Valerie
authors: V
authors: Girling
authors: Peilin
authors: P
authors: Li
authors: Diane V
authors: DV
authors: Havlir
authors: Steven G
authors: SG
authors: Deeks
authors: Joseph K
authors: JK
authors: Wong
authors: Hiroyu
authors: H
authors: Hatano
mesh_terms: D001706:Biopsy; D015496:CD4-Positive T-Lymphocytes / Q000821:virology*; D004279:DNA, Viral / Q000302:isolation & purification*; D006678:HIV / Q000302:isolation & purification*; D015658:HIV Infections / Q000821:virology*; D006801:Humans; D022423:Myeloid Cells / Q000821:virology*; D012007:Rectum / Q000821:virology*; D019562:Viral Load
chemical_list: D004279:DNA, Viral