title: Preliminary experience with the use of an autologous platelet-rich fibrin membrane for urethroplasty coverage in distal hypospadias surgery.
abstract: OBJECTIVE
  Platelet-rich fibrin (PRF) has been shown to have structural and biological properties that promote tissue healing. This prospective study evaluated the feasibility, safety, and efficiency of using autologous PRF membrane for urethroplasty coverage in distal hypospadias.
  
  
  METHODS
  We prospectively included 33 patients with distal hypospadias operated on between June 2010 and September 2011. Urethroplasties were performed using the Duplay technique. During surgery, 5-10 ml of patient's blood was collected and immediately centrifuged. A PRF clot was transformed into a dense fibrin membrane with a particular cell content and architecture. This membrane was applied and sutured over the urethroplasty. The perioperative course and complications were recorded. Outcomes were compared with those in a control group of children undergoing the same procedure, but with another mean of coverage.
  
  
  RESULTS
  With a median follow-up of 8 months (range, 6-18 months), urethral fistula occurred in 2/33 patients. No other complication was noted. No complication related to the blood sampling was reported. There was no statistically significant difference with the control group (p = 0.65).
  
  
  CONCLUSIONS
  The PRF patch seems to be a safe and efficient covering technique. Thus, procedure is an additional approach to coverage for hypospadias surgery, and may help to reduce the incidence of postoperative complications when coverage healthy tissue is not available.
authors: Department of Pediatric Surgery, University Hospital of Rennes, F-35000, France. Electronic address: audrey.guinot@chu-rennes.fr.
authors: A
authors: A
authors: Guinot
authors: Department of Pediatric Surgery, University Hospital of Rennes, F-35000, France.
authors: A
authors: A
authors: Arnaud
authors: Department of Pediatric Surgery, University Hospital of Rennes, F-35000, France.
authors: O
authors: O
authors: Azzis
authors: Department of Pediatric Surgery, University Hospital of Rennes, F-35000, France.
authors: E
authors: E
authors: Habonimana
authors: Department of Pediatric Surgery, University Hospital of Rennes, F-35000, France.
authors: S
authors: S
authors: Jasienski
authors: Department of Pediatric Surgery, University Hospital of Rennes, F-35000, France.
authors: B
authors: B
authors: Frémond
mesh_terms: D016022:Case-Control Studies; D005500:Follow-Up Studies; D006801:Humans; D007021:Hypospadias / Q000175:diagnosis / Q000601:surgery*; D007223:Infant; D008297:Male; D008567:Membranes, Artificial; D053657:Platelet-Rich Plasma; D011446:Prospective Studies; D019651:Plastic Surgery Procedures / Q000379:methods*; D018570:Risk Assessment; D023822:Tissue Engineering; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D014521:Urethra / Q000601:surgery*; D013521:Urologic Surgical Procedures, Male / Q000379:methods; D014945:Wound Healing / Q000502:physiology*
chemical_list: D008567:Membranes, Artificial
keywords: Autologous; Duplay; Hypospadias; Platelet rich fibrin; Urethrocutaneous fistula