title: Analysis of cell hyperplasia and parietal cell dysfunction induced by Ostertagia ostertagi infection.
abstract: Infections in cattle with the gastric nematode Ostertagia ostertagi are associated with decreased acid secretion and profound physio-morphological changes of the gastric mucosa. The purpose of the current study was to investigate the mechanisms triggering these pathophysiological changes. O. ostertagi infection resulted in a marked cellular hyperplasia, which can be explained by increased transcriptional levels of signaling molecules related to the homeostasis of gastric epithelial cells such as HES1, WNT5A, FGF10, HB-EGF, AREG, ADAM10 and ADAM17. Intriguingly, histological analysis indicated that the rapid rise in the gastric pH, observed following the emergence of adult worms, cannot be explained by a loss of parietal cells, as a decrease in the number of parietal cells was only observed following a long term infection of several weeks, but is likely to be caused by an inhibition of parietal cell activity. To investigate whether this inhibition is caused by a direct effect of the parasites, parietal cells were co-cultured with parasite Excretory/Secretory products (ESP) and subsequently analyzed for acid production. The results indicate that adult ESP inhibited acid secretion, whereas ESP from the L4 larval stages did not alter parietal cell function. In addition, our data show that the inhibition of parietal cell activity could be mediated by a marked upregulation of inflammatory factors, which are partly induced by adult ESP in abomasal epithelial cells. In conclusion, this study shows that the emergence of adult O. ostertagi worms is associated with marked cellular changes that can be partly triggered by the worm's Excretory/secretory antigens.
authors: Belgacem
authors: B
authors: Mihi
authors: Frederik
authors: F
authors: Van Meulder
authors: Manuela
authors: M
authors: Rinaldi
authors: Stefanie
authors: S
authors: Van Coppernolle
authors: Koen
authors: K
authors: Chiers
authors: Wim
authors: W
authors: Van den Broeck
authors: Bruno
authors: B
authors: Goddeeris
authors: Jozef
authors: J
authors: Vercruysse
authors: Edwin
authors: E
authors: Claerebout
authors: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium. peter.geldhof@ugent.be.
authors: Peter
authors: P
authors: Geldhof
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology / Q000503:physiopathology*; D005753:Gastric Mucosa / Q000276:immunology / Q000469:parasitology / Q000503:physiopathology*; D007814:Larva / Q000254:growth & development / Q000502:physiology; D010028:Ostertagia / Q000254:growth & development / Q000502:physiology*; D010029:Ostertagiasis / Q000276:immunology / Q000469:parasitology / Q000503:physiopathology / Q000662:veterinary*; D010295:Parietal Cells, Gastric / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D011897:Random Allocation; D015398:Signal Transduction