title: Poly(vinylidene-trifluoroethylene)/barium titanate composite for in vivo support of bone formation.
abstract: In this study, we evaluated the effect of poly(vinylidene fluoride-trifluoroethylene)/barium titanate (P(VDF-TrFE)/BT) membrane on in vivo bone formation. Rat calvarial bone defects were implanted with P(VDF-TrFE)/BT and polytetrafluoroethylene (PTFE) membranes, and at 4 and 8 weeks, histomorphometric and gene expression analyses were performed. A higher amount of bone formation was noticed on P(VDF-TrFE)/BT compared with PTFE. The gene expression of RUNX2, bone sialoprotein, osteocalcin, receptor activator of nuclear factor-kappa B ligand, and osteoprotegerin indicates that P(VDF-TrFE)/BT favored the osteoblast differentiation compared with PTFE. These results evidenced the benefits of using P(VDF-TrFE)/BT to promote new bone formation, which may represent a promising alternative to be employed in guided bone regeneration.
authors: Cell Culture Laboratory, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors: Helena B
authors: HB
authors: Lopes
authors: Cell Culture Laboratory, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors: Thiago de S
authors: Tde S
authors: Santos
authors: Cell Culture Laboratory, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors: Fabiola S
authors: FS
authors: de Oliveira
authors: Cell Culture Laboratory, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors: Gileade P
authors: GP
authors: Freitas
authors: Cell Culture Laboratory, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors: Adriana Lg
authors: AL
authors: de Almeida
authors: Institute of Physics and Chemistry, Federal University of Itajubá, Itajubá, MG, Brazil.
authors: Rossano
authors: R
authors: Gimenes
authors: Cell Culture Laboratory, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors: Adalberto L
authors: AL
authors: Rosa
authors: Cell Culture Laboratory, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil mmbeloti@usp.br.
authors: Marcio M
authors: MM
authors: Beloti
mesh_terms: D000818:Animals; D017609:Barium Compounds / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000737:chemistry; D015870:Gene Expression; D048091:Guided Tissue Regeneration / Q000379:methods*; D006845:Hydrocarbons, Fluorinated / Q000737:chemistry; D021621:Imaging, Three-Dimensional; D008422:Materials Testing; D008567:Membranes, Artificial; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D014025:Titanium / Q000737:chemistry; D014753:Vinyl Compounds / Q000737:chemistry; D055114:X-Ray Microtomography
chemical_list: D017609:Barium Compounds; D001672:Biocompatible Materials; D018786:Bone Substitutes; D006845:Hydrocarbons, Fluorinated; D008567:Membranes, Artificial; D014753:Vinyl Compounds; C522521:poly(vinylidenefluoride-trifluoroethylene); C024547:barium titanate(IV); D014025:Titanium
keywords: Bone; ceramic; guided bone regeneration; membrane; osteoblast; polymer