title: MicroRNA-344 inhibits 3T3-L1 cell differentiation via targeting GSK3β of Wnt/β-catenin signaling pathway.
abstract: Differentiation of 3T3-L1 cells into adipocytes involves a highly orchestrated series of complex events in which microRNAs might play an essential role. In this study, we found that the overexpression of microRNA-344 (miR-344) inhibits 3T3-L1 cell differentiation and decreases triglyceride accumulation after MDI stimulation. We demonstrated that miR-344 directly targets the 3' UTR of GSK3β (Glycogen synthase kinase 3 beta). Knockdown of GSK3β with siRNA results in inhibiting 3T3-L1 differentiation, while its overexpression restores the effect of miR-344. In addition, miR-344 elevates the level of active β-catenin, which is the downstream effector of GSK3β in the Wnt/β-catenin signaling pathway. These data indicate that miR-344 inhibits adipocyte differentiation via targeting GSK3β and subsequently activating the Wnt/β-catenin signaling pathway.
authors: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, PR China.
authors: Hu
authors: H
authors: Chen
authors: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, PR China.
authors: Siqi
authors: S
authors: Wang
authors: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, PR China.
authors: Luxi
authors: L
authors: Chen
authors: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, PR China.
authors: Yaosheng
authors: Y
authors: Chen
authors: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, PR China.
authors: Ming
authors: M
authors: Wu
authors: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, PR China.
authors: Yun
authors: Y
authors: Zhang
authors: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, PR China.
authors: Kaifan
authors: K
authors: Yu
authors: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, PR China.
authors: Zheng
authors: Z
authors: Huang
authors: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, PR China.
authors: Lijun
authors: L
authors: Qin
authors: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, PR China. Electronic address: modelin@mail.sysu.edu.cn.
authors: Delin
authors: D
authors: Mo
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes; D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D038362:Glycogen Synthase Kinase 3 / Q000037:antagonists & inhibitors / Q000235:genetics*; D000071679:Glycogen Synthase Kinase 3 beta; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D047495:PPAR gamma / Q000235:genetics; D060449:Wnt Signaling Pathway / Q000235:genetics*; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: D035683:MicroRNAs; C587331:Mirn344 microRNA, mouse; D047495:PPAR gamma; D051176:beta Catenin; C000605506:GSK3B protein, human; D000071679:Glycogen Synthase Kinase 3 beta; C000605507:Gsk3b protein, mouse; D038362:Glycogen Synthase Kinase 3
keywords: Adipogenesis; GSK3β; Wnt/β-catenin signaling; miR-344