title: Metabolic profiling of amino acids in cellular samples via zwitterionic sub-2 μm particle size HILIC-MS/MS and a uniformly 13C labeled internal standard.
abstract: A novel analytical method using hydrophilic interaction liquid chromatography combined with electrospray tandem mass spectrometry for metabolic profiling of free, underivatized amino acids is presented. The separation uses a zwitterionic modified silica-based stationary phase with 1.8-μm particle size functionalized with ammonium sulfonic acid groups. Quantification is based on external standard calibration using a Pichia pastoris cell extract grown on uniformly (13)C labeled glucose as an internal standard. The absolute limits of detection in the cellular matrix were in the subpicomolar range. Measurement accuracy was assessed by analyzing NIST Standard Reference Material 2389a, which provides certified values for 17 amino acids. The recovery of the amino acids ranged between 65 % (proline) and 120 % (lysine), with excellent repeatability precision below 2.5 % (n = 5). Only, cystine showed poor recovery (29 %) and repeatability precision (13 %). Generally, the long-term precision obtained by hydrophilic interaction liquid chromatography-tandem mass spectrometry was excellent, being on average less than 9 % over 20 h of measurement time. Moreover, the novel separation method had average repeatability and reproducibility of the chromatographic peak width over time periods of 20 h and 6 months of 8 and 15 %, respectively, demonstrating its high robustness in routine analysis of cellular samples. Large concentration differences depending on the amino acid were found in the cell extracts, typically ranging from 0.002 nmol per milligram of cell dry weight (cystine) to 56 nmol per milligram of cell dry weight (arginine and glutamic acid).
authors: Austrian Centre of Industrial Biotechnology, Muthgasse 11, 1190, Vienna, Austria.
authors: R
authors: R
authors: Guerrasio
authors: C
authors: C
authors: Haberhauer-Troyer
authors: D
authors: D
authors: Mattanovich
authors: G
authors: G
authors: Koellensperger
authors: S
authors: S
authors: Hann
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D002138:Calibration; D002247:Carbon Isotopes / Q000032:analysis*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002853:Chromatography, Liquid; D005656:Fungal Proteins / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D057230:Limit of Detection; D055442:Metabolome; D010316:Particle Size; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: D000596:Amino Acids; D002247:Carbon Isotopes; D005656:Fungal Proteins