title: Identification of volatile markers in potato brown rot and ring rot by combined GC-MS and PTR-MS techniques: study on in vitro and in vivo samples.
abstract: Ralstonia solanacearum (Rs) and Clavibacter michiganensis subsp. sepedonicus (Cms) are the bacterial causal agents of potato brown and ring rot, respectively, and are included in the A2 list of quarantine pathogens in Europe. Identification by GC-MS analysis of volatile organic compounds from Rs or Cms cultured on different nutrient media was performed. GC-MS and PTR-MS analysis were carried out also on unwounded potato tubers infected with the same pathogens. Infected tubers were produced by experimental inoculations of the plants. In in vitro experiments, Rs or Cms emitted volatile compounds, part of which were specific disease markers of potato (2-propanol and 3-methylbutanoic acid), mainly originating from bacterial metabolism (i.e., amino acid degradation, carbohydrate and fatty acid oxidation). In potato tubers, pathogen metabolism modified the volatile compound pattern emitted from healthy samples. Both bacteria seem to accelerate metabolic processes ongoing in potatoes and, in the case of Rs, disease markers (1-hepten-3-ol, 3,6-dimethyl-3-octanone, 3-ethyl-3-methylpentane, 1-chloroctane, and benzothiazole) were identified.
authors: Department of Agricultural Sciences, University of Bologna , Viale G. Fanin 44, 40127 Bologna, Italy.
authors: Sonia
authors: S
authors: Blasioli
authors: Enrico
authors: E
authors: Biondi
authors: Devasena
authors: D
authors: Samudrala
authors: Francesco
authors: F
authors: Spinelli
authors: Antonio
authors: A
authors: Cellini
authors: Assunta
authors: A
authors: Bertaccini
authors: Simona M
authors: SM
authors: Cristescu
authors: Ilaria
authors: I
authors: Braschi
mesh_terms: D019840:2-Propanol / Q000032:analysis; D039903:Actinobacteria / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D045782:Hemiterpenes; D013058:Mass Spectrometry / Q000379:methods*; D010421:Pentanoic Acids / Q000032:analysis; D010935:Plant Diseases / Q000382:microbiology*; D035281:Plant Tubers / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D043368:Ralstonia solanacearum / Q000378:metabolism; D011198:Solanum tuberosum / Q000382:microbiology*; D055549:Volatile Organic Compounds / Q000032:analysis*
chemical_list: D015415:Biomarkers; D045782:Hemiterpenes; D010421:Pentanoic Acids; D055549:Volatile Organic Compounds; C008216:isovaleric acid; D019840:2-Propanol
keywords: Clavibacter michiganensis subsp. sepedonicus; Ralstonia solanacearum; volatile compounds