title: Resveratrol suppresses inflammatory responses in endometrial stromal cells derived from endometriosis: a possible role of the sirtuin 1 pathway.
abstract: OBJECTIVE
  Endometriosis is a chronic inflammatory disease. Sirtuin 1 (SIRT1) plays a role in regulation of inflammation. The role of SIRT1 in endometriosis remains unknown. We here addressed the anti-inflammatory effects of SIRT1 on endometriosis.
  
  
  METHODS
  The expression of SIRT1 in human ovarian endometriomas and eutopic endometria were examined using immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR). Endometriotic stromal cells (ESC) obtained from endometriomas were exposed to either resveratrol or sirtinol, an activator or inhibitor of sirtuins, respectively, and tumor necrosis factor (TNF)-α-induced interleukin (IL)-8 release from the ESC was assessed at mRNA and protein levels.
  
  
  RESULTS
  Both immunochemistry and RT-PCR demonstrated that SIRT1 was expressed in ESC and normal endometrial stromal cells. Resveratrol suppressed TNF-α-induced IL-8 release from the ESC in a dose-dependent manner while sirtinol increased IL-8 release.
  
  
  CONCLUSIONS
  These opposing effects of SIRT1-related agents suggest that IL-8 release from the ESC is modulated through the SIRT1 pathway. Resveratrol may have the potential to ameliorate local inflammation in endometriomas.
authors: Department of Obstetrics and Gynecology, Faculty of Medicine, The University of Tokyo, Tokyo, Japan.
authors: Ayumi
authors: A
authors: Taguchi
authors: Osamu
authors: O
authors: Wada-Hiraike
authors: Kei
authors: K
authors: Kawana
authors: Kaori
authors: K
authors: Koga
authors: Aki
authors: A
authors: Yamashita
authors: Akira
authors: A
authors: Shirane
authors: Yoko
authors: Y
authors: Urata
authors: Shiro
authors: S
authors: Kozuma
authors: Yutaka
authors: Y
authors: Osuga
authors: Tomoyuki
authors: T
authors: Fujii
mesh_terms: D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001549:Benzamides / Q000494:pharmacology; D002478:Cells, Cultured; D004715:Endometriosis / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D004717:Endometrium / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D020536:Enzyme Activators / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D009284:Naphthols / Q000494:pharmacology; D010049:Ovarian Diseases / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D010053:Ovary / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D000077185:Resveratrol; D015398:Signal Transduction / Q000187:drug effects; D056564:Sirtuin 1 / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology*; D017154:Stromal Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D001549:Benzamides; C508606:CXCL8 protein, human; D020536:Enzyme Activators; D004791:Enzyme Inhibitors; D016209:Interleukin-8; D009284:Naphthols; D013267:Stilbenes; C439060:sirtinol; C447939:SIRT1 protein, human; D056564:Sirtuin 1; D000077185:Resveratrol
keywords: endometriosis; inflammation; interleukin-8; resveratrol; sirtinol; tumor necrosis factor-α