title: Hypoxia-inducible factor-1α and its role in the proliferation of retinoblastoma cells.
abstract: In order to better understand the role of HIF-1α in the proliferation of the retinoblastoma cells, a siRNA knockdown of HIF-1α followed by a proliferation assay was performed. Further sequencing was then carried out in order to assess knockdown efficiency and expression of HIF-1α. Upregulation of HIF-1α gene expression in CoCl2-treated retinoblastoma cells was demonstrated via melting curve analysis from PCR tests and was further analyzed using western blot and densitometry analysis. Reduction of HIF-1α expression in retinoblastoma, post HIF-1α knockdown, was observed after siRNA transfection into Y-79 cells. Knockdown of HIF-1α resulted in a significant decrease in proliferation thereby demonstrating that HIF-1α is involved in promoting survival and proliferation in retinoblastoma cells. Stabilization of HIF-1α in retinoblastoma cells using CoCl2 was unsuccessful.
authors: Department of Ophthalmology and Pathology, The McGill University Health Center & Henry C. Witelson Ocular Pathology Laboratory, 3775 University St., Room 216, Montreal, QC, Canada, H3A 2B4, bruno.mtl@gmail.com.
authors: Bruno F
authors: BF
authors: Fernandes
authors: James
authors: J
authors: Coates
authors: Alexandre N
authors: AN
authors: Odashiro
authors: Carlos
authors: C
authors: Quezada
authors: Aimee
authors: A
authors: Huynh
authors: Patricia R
authors: PR
authors: Odashiro
authors: Macanori
authors: M
authors: Odashiro
authors: Miguel N
authors: MN
authors: Burnier
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D015687:Cell Hypoxia; D049109:Cell Proliferation; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D019572:Retinal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D012175:Retinoblastoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D034741:RNA, Small Interfering