title: Effects of fluoride on bacterial growth and its gene/protein expression.
abstract: To determine the effects of fluoride on bacterial growth, as well as upon its gene/protein expression, we grew Escherichia coli expressing GFPuv (E. coli-GFPuv) in Luria Bertani medium at different concentrations of NaF, 0, 0.1 mM, 1 mM, 10 mM and 100 mM. Results showed that E. coli-pGFPuv growth and expression of mRNA and protein of GFPuv were increased at 0.1 and 1 mM, but were inhibited at 10 and 100 mM, which demonstrated that fluoride has a classic rise/fall response of inducing E. coli-GFPuv growth and gene and protein expression of GFPuv at 1 mM. Our observation suggests that the effect of fluoride on bacterial growth may be from regulation of mRNA expression.
authors: Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, PR China.
authors: Haili
authors: H
authors: Ma
authors: Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, PR China.
authors: Xiaohu
authors: X
authors: Wu
authors: Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, PR China.
authors: Meng
authors: M
authors: Yang
authors: Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, PR China.
authors: Jianmei
authors: J
authors: Wang
authors: Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, PR China.
authors: Jinming
authors: J
authors: Wang
authors: Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, PR China. Electronic address: wangjd@sxau.edu.cn.
authors: Jundong
authors: J
authors: Wang
mesh_terms: D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects* / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012969:Sodium Fluoride / Q000494:pharmacology*
chemical_list: D012329:RNA, Bacterial; D049452:Green Fluorescent Proteins; D012969:Sodium Fluoride
keywords: Escherichia coli; Expression; Fluoride; GFPuv; Growth