title: Pro-inflammatory cytokine release and cell growth inhibition in primary human oral cells after exposure to endodontic sealer.
abstract: OBJECTIVE
  To assay the toxicity of the single-methacrylate-based sealer urethane dimethacrylate (UDMA) (EndoRez) in terms of cell growth and pro-inflammatory cytokines release, in expanded ex vivo human dental pulp stem cells (hDPSCs), human periodontal ligament stem cells (hPDLSCs), human gingival fibroblasts (hGFs) and human osteoblasts (hOSTs).
  
  
  METHODS
  Dental pulp and periodontal ligament stem cells, osteoblasts and fibroblasts were derived from five young donors. After in vitro isolation, hDPSCs, hPDLSCs, hGFs and hOSTs were seeded to resin-based sealers for 24, 48, 72 h up to 1 week. The morphological features and the cell growth and the release of pro-inflammatory interleukin (IL)6, IL8, IL12 and tumour necrosis factor (TNF) α were analysed. Differences in cell growth and in interleukin secretion were analysed for statistical significance with two-way anova tests for multiple comparisons.
  
  
  RESULTS
  Exposure to endodontic sealer based on UDMA resulted in a 50% decrease in survival oral cells at 24 h of incubation. No evident morphological changes were present in cell cultures examined. After 48 h, 72 h and 1-week culture time, a progressive cell growth was evident. A significant up-regulation of IL6, IL8, IL12 and TNFα cytokines in cells in contact with the dental sealer compared to the control was observed.
  
  
  CONCLUSIONS
  In vitro, EndoRez interacted with primary human hDPSCs, hPDLSCs, hGFs and hOSTs causing damage to biological system evidenced through cell growth inhibition and up-regulation of IL6, IL8, IL12 and TNFα proinflammatory mediators.
authors: Department of Medical, Oral and Biotechnological Sciences, University 'G. d'Annunzio', Chieti-Pescara, Italy.
authors: F
authors: F
authors: Diomede
authors: S
authors: S
authors: Caputi
authors: I
authors: I
authors: Merciaro
authors: S
authors: S
authors: Frisone
authors: C
authors: C
authors: D'Arcangelo
authors: A
authors: A
authors: Piattelli
authors: O
authors: O
authors: Trubiani
mesh_terms: D002455:Cell Division; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D009055:Mouth / Q000166:cytology / Q000378:metabolism*; D012387:Root Canal Filling Materials
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D012387:Root Canal Filling Materials
keywords: cytokines; endodontic sealer; human oral cells; inflammation