title: Preparation and characterization of lysine-immobilized poly(glycidyl methacrylate) nanoparticle-coated capillary for the separation of amino acids by open tubular capillary electrochromatography.
abstract: In this study, poly(glycidyl methacrylate) (PGMA) nanoparticles (NPs) were prepared and chemically immobilized for the first time onto a capillary inner wall for open tubular capillary electrochromatography (OTCEC). The immobilization of PGMA NPs onto the capillary was attained by a ring-opening reaction between the NPs and an amino-silylated fused capillary inner surface. Scanning electron micrographs clearly demonstrated that the NPs were bound to the capillary inner surface in a dense monolayer. The PGMA NP-coated column was then functionalized by lysine (Lys). After fuctionalization, the capillary can afford strong anodic electroosmotic flow, especially in acidic running buffers. Separations of three amino acids (including tryptophan, tyrosine and phenylalanine) were performed in NP-modified, monolayer Lys-functionalized and bare uncoated capillaries. Results indicated that the NP-coated column can provide more retention and higher resolution for analytes due to the hydrophobic interaction between analytes and the NP-coating. Run-to-run and column-to-column reproducibilities in the separation of the amino acids using the NP-modified column were also demonstrated.
authors: Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, PR China. Electronic address: xuliang@tmu.edu.cn.
authors: Liang
authors: L
authors: Xu
authors: Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, PR China.
authors: Pengfei
authors: P
authors: Cui
authors: Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, PR China.
authors: Dongmei
authors: D
authors: Wang
authors: Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, PR China.
authors: Cheng
authors: C
authors: Tang
authors: Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, PR China.
authors: Linyi
authors: L
authors: Dong
authors: School of Food & Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China. Electronic address: zhangcan@mail.ujs.edu.cn.
authors: Can
authors: C
authors: Zhang
authors: Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, PR China.
authors: Hongquan
authors: H
authors: Duan
authors: Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, PR China; Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109-1065, USA.
authors: Victor C
authors: VC
authors: Yang
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D053801:Capillary Electrochromatography / Q000379:methods*; D053841:Electroosmosis; D008239:Lysine / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D011109:Polymethacrylic Acids; C042535:polyglycidyl methacrylate; D008239:Lysine
keywords: Amino acids; Capillary electrochromatography; Nanoparticle; Poly(glycidyl methacrylate); Separation