title: Expression profiles of genes involved in apoptosis and selenium metabolism in articular cartilage of patients with Kashin-Beck osteoarthritis.
abstract: Kashin-Beck disease (KBD) is a special type of endemic osteoarthritis. It has been suggested that alterations in selenium metabolism and apoptosis play a role in KBD. However, the underlying molecular mechanism remains largely unclear. We performed a microarray analysis using RNA isolated from cartilages of KBD patients and healthy controls, through Significance Analysis of Microarray (SAM) software. Functional gene networks and crucial molecules associated with differentially expressed genes were investigated via Ingenuity Pathway Analysis (IPA) and hub gene analysis. Quantitative real-time PCR was used to check the validation of chip test. We identified 52 up-regulated apoptosis-related genes and 26 down-regulated selenium-related genes between KBD and controls, and these genes associated with the "MYC-mediated apoptosis signaling pathway". We confirmed the results from array studies with quantitative real-time PCR analysis. Our results suggest that abnormal regulation of selenium metabolism and apoptosis through the MYC mediated signaling pathway contributes to the pathogenesis of KBD, but the relationship between apoptosis gene and selenium gene was not found.
authors: Faculty of Public Health, College of Medicine of Xi'an Jiaotong University, Key Laboratory of Environment and Gene Related Diseases of Ministry Education, Key Laboratory of Trace Elements and Endemic Diseases, Ministry of Health, Xi'an 710061, China; Department of Orthopedics Surgery, The First Affiliated Hospital, College of Medicine of Xi'an Jiaotong University, Xi'an 710061, China.
authors: Shi-Xun
authors: SX
authors: Wu
authors: Department of Orthopedics Surgery, The Second Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710004, China.
authors: Wei-Zhuo
authors: WZ
authors: Wang
authors: Faculty of Public Health, College of Medicine of Xi'an Jiaotong University, Key Laboratory of Environment and Gene Related Diseases of Ministry Education, Key Laboratory of Trace Elements and Endemic Diseases, Ministry of Health, Xi'an 710061, China.
authors: Feng
authors: F
authors: Zhang
authors: Faculty of Public Health, College of Medicine of Xi'an Jiaotong University, Key Laboratory of Environment and Gene Related Diseases of Ministry Education, Key Laboratory of Trace Elements and Endemic Diseases, Ministry of Health, Xi'an 710061, China.
authors: Cui-Yan
authors: CY
authors: Wu
authors: Faculty of Public Health, College of Medicine of Xi'an Jiaotong University, Key Laboratory of Environment and Gene Related Diseases of Ministry Education, Key Laboratory of Trace Elements and Endemic Diseases, Ministry of Health, Xi'an 710061, China.
authors: Bannel S
authors: BS
authors: Dennis
authors: Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland; Biocenter Kuopio, University of Eastern Finland, Kuopio, Finland.
authors: Cheng-Juan
authors: CJ
authors: Qu
authors: Department of Cellular and Structural Biology, University of Texas Health Sciences Center at San Antonio, 7703 Floyd Curl, San Antonio, TX 78229, USA.
authors: Yi-Dong
authors: YD
authors: Bai
authors: Faculty of Public Health, College of Medicine of Xi'an Jiaotong University, Key Laboratory of Environment and Gene Related Diseases of Ministry Education, Key Laboratory of Trace Elements and Endemic Diseases, Ministry of Health, Xi'an 710061, China. Electronic address: Guox@mail.xjtu.edu.cn.
authors: Xiong
authors: X
authors: Guo
mesh_terms: D000328:Adult; D017209:Apoptosis / Q000235:genetics*; D002358:Cartilage, Articular / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D006801:Humans; D057767:Kashin-Beck Disease / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000235:genetics* / Q000378:metabolism; D012643:Selenium / Q000378:metabolism*; D015398:Signal Transduction; D059467:Transcriptome
chemical_list: D012643:Selenium
keywords: BIND; Biomolecular Interaction Network Database; Cartilage; DIP; Database of Interacting Proteins; FDR; GPX; HPRD; Hub gene; Human Protein Reference Database; IPA; Ingenuity Pathway Analysis; KBD; Kashin–Beck disease; MINT; Molecular Interaction database; PDI; PPI; SAM; Signaling pathway; Significance Analysis of Microarray; false discovery rate; glutathione peroxidases; protein–DNA interaction; protein–protein interaction