title: Agronomic and genetic analysis of Suweon 542, a rice floury mutant line suitable for dry milling.
abstract: BACKGROUND
  Producing rice flour of good quality by dry milling is necessary to reduce milling costs and promote the processed rice food industry. This study was conducted to evaluate the dry milling properties of Suweon 542, a floury endosperm mutant, and identify the chromosomal region responsible for the floury endosperm characteristics.
  
  
  RESULTS
  Compared with the wild type, after dry milling process, the grain hardness of Suweon 542 was significantly lower because of its round and loosely packed starch granules. Also, the flour of Suweon 542 had significantly smaller particles and less damaged starch than Namil and other rice cultivars and its particle size distribution was similar to a commercial wheat cultivar. Considering that the yield loss of Suweon 542 due to its floury endosperm was largely compensated for by an increased number of spikelets per panicle, Suweon 542 has potential value as a raw material for rice flour production. Association analysis using 70 genome-wide SSR markers and 94 F2 plants derived from Suweon 542/Milyang 23 showed that markers on chromosome 5 explained a large portion of the variation in floury grains percentage (FGP). Further analysis with an increased number of SSR markers revealed that the floury endosperm of Suweon 542 was directed by a major recessive locus, flo7(t), located in the 19.33-19.86 Mbp region of chromosome 5, with RM18639 explaining 92.2% of FGP variation in the F2 population.
  
  
  CONCLUSIONS
  The floury endosperm of Suweon 542 is suitable for dry milling, with a small flour particle size and low damaged starch content. Further physical mapping of flo7(t), the floury endosperm locus of Suweon 542, would facilitate efficient breeding of rice cultivars with proper dry milling adaptability that can be used in the processed rice food industry.
authors: Young-Jun
authors: YJ
authors: Mo
authors: National Institute of Crop Science, Rural Development Administration, Suwon 441-857, Republic of Korea. jrnj@korea.kr.
authors: Ji-Ung
authors: JU
authors: Jeung
authors: Young-Seop
authors: YS
authors: Shin
authors: Chul Soo
authors: CS
authors: Park
authors: Kyung-Ho
authors: KH
authors: Kang
authors: Bo-Kyeong
authors: BK
authors: Kim