title: Acetaldehyde detoxification using resting cells of recombinant Escherichia coli overexpressing acetaldehyde dehydrogenase.
abstract: Acetaldehyde dehydrogenase (E.C. 1.2.1.10) plays a key role in the acetaldehyde detoxification. The recombinant Escherichia coli cells producing acetaldehyde dehydrogenase (ist-ALDH) were applied as whole-cell biocatalysts for biodegradation of acetaldehyde. Response surface methodology (RSM) was employed to enhance the production of recombinant ist-ALDH. Under the optimum culture conditions containing 20.68 h post-induction time, 126.75 mL medium volume and 3 % (v/v) inoculum level, the maximum ist-ALDH activity reached 496.65 ± 0.81 U/mL, resulting in 12.5-fold increment after optimization. Furthermore, the optimum temperature and pH for the catalytic activity of wet cells were 40 °C and pH 9.5, respectively. The biocatalytic activity was improved 80 % by permeabilizing the recombinant cells with 0.075 % (v/v) Triton X-100. When using 2 mmol/L NAD(+) as coenzyme, the permeabilized cells could catalyze 98 % of acetaldehyde within 15 min. The results indicated that the recombinant E. coli with high productivity of ist-ALDH might be highly efficient and easy-to-make biocatalysts for acetaldehyde detoxification.
authors: College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, People's Republic of China.
authors: Zhengying
authors: Z
authors: Yao
authors: Chong
authors: C
authors: Zhang
authors: Junfeng
authors: J
authors: Zhao
authors: Fengxia
authors: F
authors: Lu
authors: Xiaomei
authors: X
authors: Bie
authors: Zhaoxin
authors: Z
authors: Lu
mesh_terms: D000079:Acetaldehyde / Q000378:metabolism*; D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D055162:Biocatalysis; D004926:Escherichia coli / Q000201:enzymology*
chemical_list: D000445:Aldehyde Oxidoreductases; C018699:aldehyde dehydrogenase (NAD(P)+); D000079:Acetaldehyde