title: Selective detection of complementarity-determining regions of monoclonal antibody by limiting protease access to the substrate: nano-surface and molecular-orientation limited proteolysis.
abstract: We report a novel method for the selective proteolysis by limiting protease access to the substrate, which we have named nano-surface and molecular-orientation limited (nSMOL) proteolysis. We focus on the identification of the Fab and quantitation of antibodies. This method successfully performed limited proteolysis on the Fab.
authors: Life Science Research Center, SHIMADZU Corporation, Kanda-Nishiki-cho, Chiyoda-ku, Tokyo 101-8448, Japan. t-shima@shimadzu.co.jp.
authors: Noriko
authors: N
authors: Iwamoto
authors: Takashi
authors: T
authors: Shimada
authors: Yukari
authors: Y
authors: Umino
authors: Chikage
authors: C
authors: Aoki
authors: Yutaka
authors: Y
authors: Aoki
authors: Taka-Aki
authors: TA
authors: Sato
authors: Akinobu
authors: A
authors: Hamada
authors: Hitoshi
authors: H
authors: Nakagama
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000378:metabolism*; D022801:Complementarity Determining Regions / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D036103:Nanotechnology / Q000379:methods*; D011487:Protein Conformation; D059748:Proteolysis; D013499:Surface Properties; D014357:Trypsin / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D022801:Complementarity Determining Regions; D014357:Trypsin