title: The phenotypic fate of bone marrow-derived stem cells in acute kidney injury.
abstract: BACKGROUND
  Despite increasing attention on the role of bone marrow derived stem cells in repair or rejuvenation of tissues and organs, cellular mechanisms of such cell-based therapy remain poorly understood.
  
  
  METHODS
  We reconstituted hematopoiesis in recipient C57BL/6J mice by transplanting syngeneic GFP(+) bone marrow (BM) cells. Subsequently, the recipients received subcutaneous injection of granulocyte-colony stimulating factor (G-CSF) and were subjected to acute renal ischemic injury. Flow cytometry and immunostaining were performed at various time points to assess engraftment and phenotype of BM derived stem cells.
  
  
  RESULTS
  Administration of G-CSF increased the release of BM derived stem cells into circulation and enhanced the ensuing recruitment of BM derived stem cells into injured kidney. During the second month post injury, migrated BM derived stem cells lost hematopoietic phenotype (CD45) but maintained the expression of other markers (Sca-1, CD133 and CD44), suggesting their potential of transdifferentiation into renal stem cells. Moreover, G-CSF treatment enhanced the phenotypic conversion.
  
  
  CONCLUSIONS
  Our work depicted a time-course dependent transition of phenotypic characteristics of BM derived stem cells, demonstrated the existence of BM derived stem cells in damaged kidney and revealed the effects of G-CSF on cell transdifferentiation.
authors: Department of Urology, Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin, China.
authors: Guowei
authors: G
authors: Feng
authors: Duo
authors: D
authors: Mao
authors: Yongzhe
authors: Y
authors: Che
authors: Weijun
authors: W
authors: Su
authors: Yuebing
authors: Y
authors: Wang
authors: Yang
authors: Y
authors: Xu
authors: Yan
authors: Y
authors: Fan
authors: Hui
authors: H
authors: Zhao
authors: Deling
authors: D
authors: Kong
authors: Yong
authors: Y
authors: Xu
authors: Zongjin
authors: Z
authors: Li
mesh_terms: D058186:Acute Kidney Injury / Q000473:pathology*; D000818:Animals; D016026:Bone Marrow Transplantation; D054338:Cell Transdifferentiation; D004195:Disease Models, Animal; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000378:metabolism / Q000502:physiology*; D007668:Kidney / Q000098:blood supply / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D015427:Reperfusion Injury / Q000473:pathology; D013997:Time Factors
chemical_list: D016179:Granulocyte Colony-Stimulating Factor