title: Down-regulation of ribosomal protein S15A mRNA with a short hairpin RNA inhibits human hepatic cancer cell growth in vitro.
abstract: Ribosomal protein s15a (RPS15A) is a highly conserved protein that promotes mRNA/ribosome interactions early in translation. Recent evidence showed that RPS15A could stimulate growth in yeast, plant and human lung carcinoma. Here we report that RPS15A knockdown could inhibit hepatic cancer cell growth in vitro. When transduced with shRPS15A-containing lentivirus, we observed inhibited cell proliferation and impaired colony formation in both HepG2 and Bel7404 cells. Furthermore, cell cycle analysis showed that HepG2 cells were arrested at the G0/G1 phase when transduced with Lv-shRPS15A. In conclusion, our findings provide for the first time the biological effects of RPS15A in hepatic cancer cell growth. RPS15A may play a prominent role in heptocarcinogenesis and serve as a potential therapeutic target in hepatocellular carcinoma.
authors: Department of Hepatobiliary Surgery, Wenzhou Central Hospital, Wenzhou 325000, China.
authors: Maiyu
authors: M
authors: Xu
authors: Environmental and Public Health School of Wenzhou Medical University, Wenzhou 325000, China.
authors: Yi
authors: Y
authors: Wang
authors: Department of Hepatobiliary Surgery, Wenzhou Central Hospital, Wenzhou 325000, China.
authors: Lei
authors: L
authors: Chen
authors: Department of Hepatobiliary Surgery, Wenzhou Central Hospital, Wenzhou 325000, China.
authors: Bujian
authors: B
authors: Pan
authors: Department of Hepatobiliary Surgery, Wenzhou Central Hospital, Wenzhou 325000, China.
authors: Feng
authors: F
authors: Chen
authors: Department of Anus & Intestine Surgery, Wenzhou Central Hospital, Wenzhou, Zhejiang 325000, China.
authors: Yang
authors: Y
authors: Fang
authors: Department of Hepatobiliary Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors: Zhengping
authors: Z
authors: Yu
authors: Department of Hepatobiliary Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China. Electronic address: gang_chendr@163.com.
authors: Gang
authors: G
authors: Chen
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects*; D015536:Down-Regulation / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D034622:RNA Interference / Q000502:physiology; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D034741:RNA, Small Interfering / Q000494:pharmacology*; D012269:Ribosomal Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012269:Ribosomal Proteins; C074488:ribosomal protein S15a
keywords: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; ATCC; American Type Culture Collection; DMEM; Dulbecco's modified Eagle's medium; FACS; FBS; GFP; Growth; HBxAg; HCC; Hepatocellular carcinoma; MTT; PBS; PI; RNA interference; RNAi; RPS15A; TGFβ1; eIF-4F; eukaryotic initiation factor 4F; fetal bovine serum; fluorescence-activated cell sorting; green fluorescence protein; hepatitis B virus×antigen; hepatocellular carcinoma; phosphate-buffered saline; propidium iodide; ribosomal protein S15A; shRNA; shRPS15A; short hairpin RNA; siRNA; small interfering RNA; transforming growth factor beta 1