title: Patchiness of ion-exchanged mica revealed by DNA binding dynamics at short length scales.
abstract: The binding of double-stranded (ds) DNA to mica can be controlled through ion-exchanging the mica with divalent cations. Measurements of the end-to-end distance of linear DNA molecules discriminate whether the binding mechanism occurs through 2D surface equilibration or kinetic trapping. A range of linear dsDNA fragments have been used to investigate length dependences of binding. Mica, ion-exchanged with Ni(II) usually gives rise to kinetically trapped DNA molecules, however, short linear fragments (<800 bp) are seen to deviate from the expected behaviour. This indicates that ion-exchanged mica is heterogeneous, and contains patches or domains, separating different ionic species. These results correlate with imaging of dsDNA under aqueous buffer on Ni(II)-mica and indicate that binding domains are of the order of 100 nm in diameter. Shorter DNA fragments behave intermediate to the two extreme cases of 2D equilibration and kinetic trapping. Increasing the incubation time of Ni(II) on mica, from minutes to hours, brings the conformations of the shorter DNA fragments closer to the theoretical value for kinetic trapping, indicating that long timescale kinetics play a role in ion-exchange. X-ray photoelectron spectroscopy (XPS) was used to confirm that the relative abundance of Ni(II) ions on the mica surface increases with time. These findings can be used to enhance spatial control of binding of DNA to inorganic surfaces with a view to patterning high densities arrays.
authors: School of Physics and Astronomy, University of Leeds, Woodhouse Lane, Leeds, West Yorkshire LS2 9JT, UK. Department of Oral Biology, School of Dentistry, University of Leeds, Woodhouse Lane, Leeds, West Yorkshire LS2 9LU, UK.
authors: D J
authors: DJ
authors: Billingsley
authors: A J
authors: AJ
authors: Lee
authors: N A B
authors: NA
authors: Johansson
authors: A
authors: A
authors: Walton
authors: L
authors: L
authors: Stanger
authors: N
authors: N
authors: Crampton
authors: W A
authors: WA
authors: Bonass
authors: N H
authors: NH
authors: Thomson
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry* / Q000378:metabolism*; D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D007474:Ion Exchange; D007700:Kinetics; D008956:Models, Chemical; D009532:Nickel / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D056951:Photoelectron Spectroscopy; D013997:Time Factors
chemical_list: D000538:Aluminum Silicates; D009532:Nickel; D004247:DNA; C011934:mica