title: Probing the specific ion effects of biocompatible hydrated choline ionic liquids on lactate oxidase biofunctionality in sensor applications.
abstract: This paper presents an extended study on the ion effects of a series of biocompatible hydrated choline based ionic liquids (ILs) on lactate oxidase (LOx), an important enzyme in biosensing technology for the in vitro detection of lactic acid. Secondary structural analysis revealed changes in the protein conformation in hydrated ILs, while thermal unfolding/aggregation dynamics showed different profiles in the presence or absence of ILs. Moreover, LOx thermally denaturised at 90 °C showed residual activity in the presence of chloride and dihydrogen phosphate anions. Kinetic and lifetime studies were also performed, providing a better understanding of the ion effects of ILs on the biocatalytic activity of the enzyme.
authors: CLARITY: Centre for Sensor Web Technologies, National Centre for Sensor Research, Dublin City University, School of Chemical Sciences, Dublin, Ireland. fernando.lopez@dcu.ie.
authors: Vincenzo F
authors: VF
authors: Curto
authors: Stefan
authors: S
authors: Scheuermann
authors: Róisín M
authors: RM
authors: Owens
authors: Vijayaraghavan
authors: V
authors: Ranganathan
authors: Douglas R
authors: DR
authors: MacFarlane
authors: Fernando
authors: F
authors: Benito-Lopez
authors: Dermot
authors: D
authors: Diamond
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D015374:Biosensing Techniques; D002794:Choline / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D007477:Ions; D019344:Lactic Acid / Q000032:analysis; D006899:Mixed Function Oxygenases / Q000737:chemistry* / Q000378:metabolism; D014867:Water / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D052578:Ionic Liquids; D007477:Ions; D014867:Water; D019344:Lactic Acid; D006899:Mixed Function Oxygenases; C021497:lactate 2-monooxygenase; D002794:Choline