title: The effect of metals on SDS-induced partially folded states of CopC.
abstract: In this work, the unfolding of CopC was used to elucidate details of the protein structure through different spectroscopic techniques. The interactions of CopC and its mutants with the anionic surfactant sodium dodecyl sulfate (SDS), guanidinium hydrochloride, and urea were monitored by fluorescence spectroscopy, far-UV circular dichroism spectroscopy, and fluorescence lifetime measurements. The interaction of SDS with CopC resulted in the formation of a partially folded intermediate. In this intermediate, the structure of the C-terminal is unfolded, whereas the N-terminal retains the native structure. Further, we have explored the effects of metals on the intermediate in aqueous surfactant. The results suggested that the Ag(+) ion has a large effect on the unfolding induced by SDS. In addition, the binding capacity of the different unfolding degree protein toward Cu(2+) indicated the high stability of the N-terminal. The protein-Cu(2+) unfolding induced by guanidinium hydrochloride and urea caused the binding of Cu(2+) to increase the stability of the N-terminal, which resulted in an intermediate in the unfolding process. The first transition corresponded to unfolding of the C-terminal, and the second transition was attributed to unfolding of the N-terminal. Furthermore, the anisotropy decay indicated that the motion of tryptophan occurred at a higher urea concentration, which suggested the high stability of the N-terminal. Steered molecular dynamics simulations also indicated that the structure of the N-terminal was rigid.
authors: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan, 030006, China.
authors: Zhen
authors: Z
authors: Song
authors: Jie
authors: J
authors: Ming
authors: Binsheng
authors: B
authors: Yang
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites / Q000187:drug effects / Q000502:physiology; D008670:Metals / Q000378:metabolism*; D017354:Point Mutation / Q000235:genetics; D017510:Protein Folding / Q000187:drug effects; D017433:Protein Structure, Secondary; D058767:Protein Unfolding / Q000187:drug effects; D012967:Sodium Dodecyl Sulfate / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; C071265:CopC protein, Bacteria; D008670:Metals; D012967:Sodium Dodecyl Sulfate