title: Anti-Müllerian hormone recruits BMPR-IA in immature granulosa cells.
abstract: Anti-Müllerian hormone (AMH) is a member of the TGF-β superfamily secreted by the gonads of both sexes. This hormone is primarily known for its role in the regression of the Müllerian ducts in male fetuses. In females, AMH is expressed in granulosa cells of developing follicles. Like other members of the TGF-β superfamily, AMH transduces its signal through two transmembrane serine/threonine kinase receptors including a well characterized type II receptor, AMHR-II. The complete signalling pathway of AMH involving Smads proteins and the type I receptor is well known in the Müllerian duct and in Sertoli and Leydig cells but not in granulosa cells. In addition, few AMH target genes have been identified in these cells. Finally, while several co-receptors have been reported for members of the TGF-β superfamily, none have been described for AMH. Here, we have shown that none of the Bone Morphogenetic Proteins (BMPs) co-receptors, Repulsive guidance molecules (RGMs), were essential for AMH signalling. We also demonstrated that the main Smad proteins used by AMH in granulosa cells were Smad 1 and Smad 5. Like for the other AMH target cells, the most important type I receptor for AMH in these cells was BMPR-IA. Finally, we have identified a new AMH target gene, Id3, which could be involved in the effects of AMH on the differentiation of granulosa cells and its other target cells.
authors: Inserm U782, Clamart, France ; Université Paris-Sud, UMR- S0782, Clamart, France.
authors: Lauriane
authors: L
authors: Sèdes
authors: Arnaud
authors: A
authors: Leclerc
authors: Hadia
authors: H
authors: Moindjie
authors: Richard L
authors: RL
authors: Cate
authors: Jean-Yves
authors: JY
authors: Picard
authors: Nathalie
authors: N
authors: di Clemente
authors: Soazik P
authors: SP
authors: Jamin
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone / Q000378:metabolism* / Q000494:pharmacology; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005341:Fibrinolysin / Q000378:metabolism; D055786:Gene Knockout Techniques; D006107:Granulosa Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D051379:Mice; D021381:Protein Transport / Q000187:drug effects; D059748:Proteolysis; D015398:Signal Transduction / Q000187:drug effects; D051898:Smad1 Protein / Q000378:metabolism; D051902:Smad5 Protein / Q000378:metabolism
chemical_list: D051898:Smad1 Protein; D051902:Smad5 Protein; D054304:Anti-Mullerian Hormone; D052005:Bone Morphogenetic Protein Receptors, Type I; D005341:Fibrinolysin