title: Collection and preparation of rodent embryonic samples for transcriptome study.
abstract: The need for large-scale collection of rodent embryos and individual embryonic tissues for genomic and proteomic studies requires modification of traditional practices of embryo necropsy. The sample intended for transcriptome study should be rapidly dissected and stabilized to preserve its molecular integrity. The retrieval of high-quality RNA, DNA, and proteins from the target tissue is crucial for informative molecular analysis (e.g., gene profiling on microarray platform). We present a reliable method of collection and preparation of rodent embryos for genomic studies supported by detailed protocols and RNA extraction results for different stages of mouse embryonic development.
authors: Histotechnology Laboratory, SAIC, Frederick National Lab of Cancer Research, NCI, NIH, Frederick, MD, USA.
authors: Yelena
authors: Y
authors: Golubeva
authors: David
authors: D
authors: Symer
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics; D004622:Embryo, Mammalian / Q000166:cytology*; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D046228:Microarray Analysis; D008967:Molecular Biology / Q000379:methods; D011247:Pregnancy; D011506:Proteins / Q000235:genetics; D012313:RNA / Q000235:genetics; D059467:Transcriptome
chemical_list: D011506:Proteins; D012313:RNA; D004247:DNA