title: Adenoviral transduction of multipotent mesenchymal stromal cells from human adipose tissue with bone morphogenetic protein BMP-2 gene.
abstract: We determined conditions for effective transduction of multipotent mesenchymal stromal cells from human adipose tissue with adenoviral constructs carrying the gene of human bone morphogenetic protein BMP-2. The peak of transgene transcription and BMP-2 protein secretion in the transduced cultures was observed on day 6 after infection. The maximum transcription of BMP-2 gene and genes of osteogenic markers (bone sialoprotein, osteopontin, and osteocalcin) was observed in the medium containing sodium β-glycerophosphate and ascorbic acid. Addition of D 3 vitamin did not enhance the expression of BMP-2 gene in transduced cells. The obtained cell cultures with high osteogenic potential can be used in bone tissue engineering.
authors: Medical Genetic Research Center, Russian Academy of Medical Sciences; ReMeTeks Company; N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia. bukharova-rmt@yandex.ru.
authors: T B
authors: TB
authors: Bukharova
authors: L V
authors: LV
authors: Logovskaya
authors: A V
authors: AV
authors: Volkov
authors: M N
authors: MN
authors: Garas
authors: E B
authors: EB
authors: Vikhrova
authors: D Yu
authors: DY
authors: Logunov
authors: O V
authors: OV
authors: Makhnach
authors: M M
authors: MM
authors: Shmarov
authors: D V
authors: DV
authors: Gol'dshtein
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D052436:Adipose Tissue, White / Q000166:cytology; D015703:Antigens, CD / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D058574:Integrin-Binding Sialoprotein / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000502:physiology; D015675:Osteocalcin / Q000378:metabolism; D053495:Osteopontin / Q000378:metabolism; D014158:Transcription, Genetic; D014161:Transduction, Genetic
chemical_list: D015703:Antigens, CD; C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D058574:Integrin-Binding Sialoprotein; D015675:Osteocalcin; D053495:Osteopontin