title: The antioxidant activity of soursop decreases the expression of a member of the NADPH oxidase family.
abstract: Cellular oxidative stress produced by an increase in free radicals is one of the factors that promote the development of chronic degenerative diseases; therefore, consuming natural antioxidants helps minimize their negative effects. This study evaluated the cytotoxicity of the soursop extract (Annona muricata), its cytoprotective capacity against oxidative stress induced by hydrogen peroxide, the inhibitory potential of reactive oxygen species (ROS), the molecular mechanism of its antioxidant action, and its capacity to repair cellular damage in the fibroblast cell line. The soursop extract proved not to be cytotoxic in fibroblast cultures and showed cytoprotective capacity against hydrogen peroxide-induced stress; in cell culture it reduced the generation of ROS significantly by inhibiting a sub-unit of the NADPH oxidase enzyme (p47phox). The soursop extract can prevent damage caused by cellular oxidants.
authors: Laboratorio de Sinovioanálisis Molecular, Instituto Nacional de Rehabilitación, Calz. México-Xochimilco No. 289, Col. Arenal de Guadalupe, C.P 14389, D.F., México. allorey@yahoo.com.
authors: Y
authors: Y
authors: Zamudio-Cuevas
authors: R
authors: R
authors: Díaz-Sobac
authors: A
authors: A
authors: Vázquez-Luna
authors: C
authors: C
authors: Landa-Solís
authors: M
authors: M
authors: Cruz-Ramos
authors: M
authors: M
authors: Santamaría-Olmedo
authors: K
authors: K
authors: Martínez-Flores
authors: A J
authors: AJ
authors: Fuentes-Gómez
authors: A
authors: A
authors: López-Reyes
mesh_terms: D016475:3T3 Cells; D000818:Animals; D031041:Annona / Q000737:chemistry*; D000975:Antioxidants / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000633:toxicity; D051379:Mice; D019255:NADPH Oxidases / Q000235:genetics* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D019255:NADPH Oxidases