title: Protective Effect against Hydroxyl-induced DNA Damage and Antioxidant Activity of Citri reticulatae Pericarpium.
abstract: OBJECTIVE
  As a typical Chinese herbal medicine, Citri reticulatae pericarpium (CRP) possesses various pharmacological effects involved in antioxidant ability. However, its antioxidant effects have not been reported yet. The objective of this work was to investigate its antioxidant ability, then further discuss the antioxidant mechanism.
  
  
  METHODS
  CRP was extracted by ethanol to obtain ethanol extract of Citri reticulatae pericarpium (ECRP). ECRP was then measured by various antioxidant methods, including DNA damage assay, DPPH assay, ABTS assay, Fe(3+)-reducing assay and Cu(2+)-reducing assay. Finally, the content of total flavonoids was analyzed by spectrophotometric method.
  
  
  RESULTS
  Our results revealed that ECRP could effectively protect against hydroxyl-induced DNA damage (IC50 944.47±147.74 μg/mL). In addition, it could also scavenge DPPH· radical (IC50349.67±1.91 μg/mL) and ABTS(+)• radical (IC5011.33±0.10 μg/mL), reduce Fe(3+) (IC50 140.95±2.15 μg/mL) and Cu(2+) (IC50 70.46±1.77 μg/mL). Chemical analysis demonstrated that the content of total flavonoids in ECRP was 198.29±12.24 mg quercetin/g.
  
  
  CONCLUSIONS
  Citri reticulatae pericarpium can effectively protect against hydroxyl-induced DNA damage. One mechanism of protective effect may be radical-scavenging which is via donating hydrogen atom (H·), donating electron (e). Its antioxidant ability can be mainly attributed to the flavonoids, especially hesperidin and narirutin.
authors: School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Waihuang East Road No.232, Guangzhou Higher Education Mega Center, 510006, Guangzhou, China.
authors: Xican
authors: X
authors: Li
authors: Yanping
authors: Y
authors: Huang
authors: Dongfeng
authors: D
authors: Chen
keywords: Antioxidant activity; Chenpi; Citri reticulatae pericarpium; DNA oxidative damage; Hesperidin; Narirutin