title: Strain dependent UV degradation of Escherichia coli DNA monitored by Fourier transform infrared spectroscopy.
abstract: In this work we present a method for detection of DNA isolated from nonpathogenic Escherichia coli strains, respectively. Untreated and UV irradiated bacterial DNAs were analyzed by FT-IR spectroscopy, to investigate their screening characteristic features and their structural radiotolerance at 253.7nm. FT-IR spectra, providing a high molecular structural information, have been analyzed in the wavenumber range 800-1800cm(-1). FT-IR signatures, spectroscopic band assignments and structural interpretations of these DNAs are reported. Also, UV damage at the DNA molecular level is of interest. Strain dependent UV degradation of DNA from E. coli has been observed. Particularly, alterations in nucleic acid bases, base pairing and base stacking have been found. Also changes in the DNA conformation and deoxyribose were detected. Based on this work, specific E. coli DNA-ligand interactions, drug development and vaccine design for a better understanding of the infection mechanism caused by an interference between pathogenic and nonpathogenic bacteria and for a better control of disease, respectively, might be further investigated using Fourier transform infrared spectroscopy. Besides, understanding the pathways for UV damaged DNA response, like nucleic acids repair mechanisms is appreciated.
authors: National Institute for Research & Development of Isotopic and Molecular Technologies, 65-103 Donath Str., 400293 Cluj-Napoca, Romania.
authors: Cristina M
authors: CM
authors: Muntean
authors: University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, 3-5 Calea Manastur Str., 400372 Cluj-Napoca, Romania.
authors: Alexandra
authors: A
authors: Lapusan
authors: Iuliu Haţieganu University of Medicine and Pharmacy, Faculty of Pharmacy, 8 Victor Babeş Str., 400012 Cluj-Napoca, Romania.
authors: Liora
authors: L
authors: Mihaiu
authors: University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, 3-5 Calea Manastur Str., 400372 Cluj-Napoca, Romania. Electronic address: rstefan@usamvcluj.ro.
authors: Razvan
authors: R
authors: Stefan
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000737:chemistry / Q000528:radiation effects*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D017550:Spectroscopy, Fourier Transform Infrared; D014466:Ultraviolet Rays
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial
keywords: DNA structure; Drug susceptibility; Escherichia coli strains; FT-IR spectroscopy; UV radiation