title: The use of plant cell wall-degrading enzymes from newly isolated Penicillium ochrochloron Biourge for viscosity reduction in ethanol production with fresh sweet potato tubers as feedstock.
abstract: Penicillium ochrochloron Biourge, which was isolated from rotten sweet potato, can produce plant cell wall-degrading enzymes (PCWDEs) with high viscosity reducing capability for ethanol production using fresh sweet potato tubers as feedstock. The enzyme preparation was characterized by a broad enzyme spectrum including 13 kinds of enzymes with the activity to hydrolyze cellulose, hemicellulose, pectin, starch, and protein. The maximum viscosity-reducing capability was observed when the enzyme preparation was obtained after 5 days of fermentation using 20 g/L corncob as a sole carbon source, 4.5 g/L NH4 NO3 as a sole nitrogen source, and an initial medium pH of 6.5. The sweet potato mash treated with the enzyme preparation exhibited much higher fermentation efficiency (92.58%) compared with commercial cellulase (88.06%) and control (83.5%). The enzyme production was then scaled up to 0.5, 5, and 100 L, and the viscosity-reducing rates were found to be 85%, 90%, and 91%, respectively. Thus, P. ochrochloron Biourge displays potential viscosity-reducing capability for ethanol production.
authors: Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, People's Republic of China.|Graduate University of the Chinese Academy of Sciences, Beijing, People's Republic of China.|Key Laboratory of Environmental and Applied Microbiology, Chinese Academy of Sciences, Chengdu, People's Republic of China.
authors: Yuhong
authors: Y
authors: Huang
authors: Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, People's Republic of China.|Key Laboratory of Environmental and Applied Microbiology, Chinese Academy of Sciences, Chengdu, People's Republic of China.
authors: Yanling
authors: Y
authors: Jin
authors: Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, People's Republic of China.|Key Laboratory of Environmental and Applied Microbiology, Chinese Academy of Sciences, Chengdu, People's Republic of China.
authors: Weiliang
authors: W
authors: Shen
authors: Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, People's Republic of China.|Key Laboratory of Environmental and Applied Microbiology, Chinese Academy of Sciences, Chengdu, People's Republic of China.
authors: Yang
authors: Y
authors: Fang
authors: Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, People's Republic of China.|Key Laboratory of Environmental and Applied Microbiology, Chinese Academy of Sciences, Chengdu, People's Republic of China.
authors: Guohua
authors: G
authors: Zhang
authors: Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, People's Republic of China.|Key Laboratory of Environmental and Applied Microbiology, Chinese Academy of Sciences, Chengdu, People's Republic of China.
authors: Hai
authors: H
authors: Zhao
mesh_terms: D001709:Biotechnology; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism*; D004789:Enzyme Activation; D004798:Enzymes / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D027723:Ipomoea batatas / Q000378:metabolism*; D010084:Oxidation-Reduction; D010407:Penicillium / Q000201:enzymology* / Q000302:isolation & purification; D059828:Plant Cells / Q000737:chemistry / Q000378:metabolism*; D035281:Plant Tubers / Q000378:metabolism*; D014783:Viscosity
chemical_list: D004798:Enzymes; D000431:Ethanol
keywords: Penicillium ochrochloron Biourge; ethanol; fungi; plant cell wall-degrading enzyme; viscosity reducing rate