title: Influence of Plasmodium vivax malaria on the relations between the osmotic stability of human erythrocyte membrane and hematological and biochemical variables.
abstract: This study evaluated the influence of infection by Plasmodium vivax on the relations between hematological and biochemical variables and the osmotic stability of the erythrocyte membrane in a Brazilian Amazon population. A total of 72 patients with P. vivax malaria were included in the study and invited to return after 14 days, post-treatment with chloroquine and primaquine, for clinical and laboratorial reevaluations. The osmotic stability of the erythrocyte membrane was analyzed by nonlinear regression of the dependency of the absorbance of hemoglobin, released with hemolysis, as a function of the salt concentration, and it was represented by the inverse of the salt concentration at the midpoint of the curve (1/H 50) and by the variation of salt concentration, which promotes lysis (dX). Bivariate and multivariate methods were used in the analysis of the results. Prior to treatment of the disease, the erythrocytes showed greater stability, probably due to the natural selection of young and also more stable erythrocytes. The bivariate analysis showed that 1/H 50 was positively correlated with red cell distribution width (RDW), urea, triglycerides, and very low-density lipoprotein (VLDL)-cholesterol, but negatively associated with albumin, HDL-cholesterol, and indirect bilirubin, while dX was negatively associated with the mean corpuscular hemoglobin concentration. These associations were confirmed by canonical correlation analysis. Stepwise multiple linear regression showed that albumin, urea, triglycerides, and VLDL-cholesterol are the variables with the highest abilities of predicting erythrocyte stability. The bivariate analysis also showed that the hematological index RDW was related to elevated levels of bilirubin and decreased levels of albumin and urea, associated with liver damage resulting from malaria.
authors: Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, 38400-902, Brazil.
authors: Rita de Cássia
authors: Rde C
authors: Mascarenhas Netto
authors: Camila
authors: C
authors: Fabbri
authors: Mariana Vaini
authors: MV
authors: de Freitas
authors: Morun
authors: M
authors: Bernardino Neto
authors: Mário Silva
authors: MS
authors: Garrote-Filho
authors: Marcus Vinícius Guimarães
authors: MV
authors: Lacerda
authors: Emerson Silva
authors: ES
authors: Lima
authors: Nilson
authors: N
authors: Penha-Silva
mesh_terms: D000328:Adult; D001663:Bilirubin / Q000737:chemistry; D001938:Brazil; D002738:Chloroquine / Q000627:therapeutic use; D004909:Erythrocyte Indices; D004910:Erythrocyte Membrane / Q000502:physiology*; D005260:Female; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D016780:Malaria, Vivax / Q000097:blood* / Q000188:drug therapy; D008297:Male; D015999:Multivariate Analysis; D009996:Osmotic Fragility; D010966:Plasmodium vivax; D014280:Triglycerides / Q000737:chemistry
chemical_list: D006454:Hemoglobins; D014280:Triglycerides; D002738:Chloroquine; D001663:Bilirubin