title: 5'-deoxy-5'-hydrazinylguanosine as an initiator of T7 Rna polymerase-catalyzed transcriptions for the preparation of labeling-ready RNAs.
abstract: 5'-deoxy-5'-hydrazinylguanosine was incorporated into the 5'-termini of RNA transcripts using T7 RNA polymerase. Transcriptions provided 5'-hydrazinyl-RNA that was readily labeled and purified. The use of fluorophore-labeled material was validated in an endoribonuclease activity assay.
authors: a Department of Chemistry and Biophysical Sciences Institute , Durham University , Durham , United Kingdom.
authors: Mark
authors: M
authors: Skipsey
authors: Gordon
authors: G
authors: Hack
authors: Thomas A
authors: TA
authors: Hooper
authors: Mark C
authors: MC
authors: Shankey
authors: Louis P
authors: LP
authors: Conway
authors: Martin
authors: M
authors: Schröder
authors: David R W
authors: DR
authors: Hodgson
mesh_terms: D017123:Bacteriophage T7 / Q000201:enzymology*; D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006151:Guanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D006834:Hydrazines / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D012313:RNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D006834:Hydrazines; D014764:Viral Proteins; D006151:Guanosine; D012313:RNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; D016650:Fluorescein-5-isothiocyanate