title: [Effect of jianpi liqi recipe on mRNA expressions of TP, DPD, and CYP3A4 in mice with human gastric cancer transplanted tumor].
abstract: OBJECTIVE
  To study the effect of Jianpi Liqi Recipe (JLR) on 5-fluorouracil (5-FU) relevant metabolic enzymes and CYP3A4 (the same enzyme of many chemotherapeutics) of mice with human gastric cancer transplanted tumor.
  
  
  METHODS
  Totally 80 mice were randomly divided into the model group, the chemotherapy group, the JLR group, and the combination group (using chemotherapy combined JLR), 20 in each group. The human gastric cancer transplanted tumor mouse model was duplicated by hypodermic inoculating MKN-8 tumor cell suspension from the left armpit. Physiological saline or JLR was given to those in the model group or the JLR group at 0.25 mL each time, twice daily by gastrogavage from the 2nd day after transplantation. Mice in the chemotherapy group were given 0.25 mL physiological saline, twice daily by gastrogavage 2 days after transplantation, for 5 days in succession, and then they were peritoneal injected with 5-FU at the daily dose of 20 mg/kg, once daily for 5 days in succession from the 7th day of transplantation. Those in the combination were given 0.25 mL JLR, twice daily by gastrogavage, for 5 days in succession, and then they were peritoneal injected with 5-FU at the daily dose of 20 mg/kg, once daily for 5 days in succession from the 7th day of transplantation. The mRNA expressions of thymidine phosphorylase (TP), dihydropyrimidine dehydrogenase (DPD), and CYP3A4 were detected using RT-PCR.
  
  
  RESULTS
  Compared with the model group and the chemotherapy group, mRNA expressions of TP and CYP3A4 obviously increased, mRNA expression of DPD obviously decreased in the JLR group and the combination group (P < 0.01). There was no statistical difference in mRNA expressions of TP, DPD, and CYP3A4 between the JLR group and the combination group (P > 0.05).
  
  
  CONCLUSIONS
  JLR could promote the activation of 5-FU, suppress the decomposition and inactivation of 5-FU in the tumor tissue of mice, and improve the chemotherapeutic efficacy through up-regulating mRNA expressions of TP and CYP3A4, and suppressing the mRNA expression of DPD.
authors: Department of Oncology, Hangzhou Hospital of Traditional Chinese Medicine, Hangzhou (310007), China. huangtin100@sohu.com
authors: Ting
authors: T
authors: Huang
authors: Xue-fei
authors: XF
authors: Yang
authors: Yong
authors: Y
authors: Li
authors: Zhi-di
authors: ZD
authors: Zhang
authors: Ling
authors: L
authors: Huang
authors: Jian-gang
authors: JG
authors: Zhang
authors: Xiao-li
authors: XL
authors: Zhang
mesh_terms: D000818:Animals; D051544:Cytochrome P-450 CYP3A / Q000235:genetics*; D042943:Dihydrouracil Dehydrogenase (NADP) / Q000235:genetics*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012333:RNA, Messenger / Q000235:genetics; D013274:Stomach Neoplasms / Q000201:enzymology / Q000235:genetics*; D013939:Thymidine Phosphorylase / Q000235:genetics*; D023041:Xenograft Model Antitumor Assays
chemical_list: D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D051544:Cytochrome P-450 CYP3A; D042943:Dihydrouracil Dehydrogenase (NADP); D013939:Thymidine Phosphorylase; C543751:Tymp protein, mouse