title: Jak-Stat signaling pathway may play a role in the pathogenesis of cholesteatoma.
abstract: OBJECTIVE
  Jak-Stat signaling pathway is one of the major signal transduction cascades which regulates most of the cellular events such as cell proliferation, differentiation, cell migration and apoptosis. This study aims to determine the activity of Jak-Stat signaling pathway in the pathogenesis of cholesteatoma.
  
  
  METHODS
  Cholesteatoma and skin samples were obtained from 10 patients who underwent tympanomastoidectomy for chronic otitis media with cholesteatoma. Immunohistochemical analysis of cholesteatoma and skin was performed using anti-Jak1, anti-Jak2, anti-Jak3, anti-Stat1, anti-Stat2, anti-Stat3, anti-Stat4 and anti-Stat5 antibodies. The immunoreactivities in cholesteatoma and skin were quantified using H-score measurement and statistical comparison was performed.
  
  
  RESULTS
  Jak1, Jak2, Jak3, Stat1 and Stat3 immunoreactivities were not detected in cholesteatoma; in contrast to the skin (129.8; 226.7; 33.0; 66.4;115.9). In addition, when H-score measurements of Stat2, Stat4 and Stat5 immunoreactivities were compared between cholesteatoma (172.8; 166.7; 120.0) and skin (400.0; 284.9; 292.0), statistically significant differences were found (p<0.0001, p<0.0001, p<0.0001).
  
  
  CONCLUSIONS
  A remarkable deficiency in the family members of Jak-Stat signaling pathway was demonstrated in cholesteatoma. Therefore, perturbations in Jak-Stat signaling pathway may play a role in the pathogenesis of cholesteatoma.
authors: Department of Otolaryngology-Head & Neck Surgery, Celal Bayar University, Manisa, Turkey. Electronic address: gorkemeskiizmir@yahoo.com.
authors: Görkem
authors: G
authors: Eskiizmir
authors: Department of Histology & Embryology, Celal Bayar University, Manisa, Turkey.
authors: H
authors: H
authors: Seda Vatansever
authors: Department of Otolaryngology-Head & Neck Surgery, Celal Bayar University, Manisa, Turkey.
authors: Erdogan
authors: E
authors: Ozgür
authors: Department of Otolaryngology-Head & Neck Surgery, Celal Bayar University, Manisa, Turkey.
authors: Asım
authors: A
authors: Aslan
authors: Department of Otolaryngology-Head & Neck Surgery, Celal Bayar University, Manisa, Turkey.
authors: Gökçe
authors: G
authors: Tanyeri
authors: Department of Otolaryngology-Head & Neck Surgery, Celal Bayar University, Manisa, Turkey.
authors: Derya
authors: D
authors: Gözüaçık
authors: Department of Histology & Embryology, Celal Bayar University, Manisa, Turkey.
authors: M
authors: M
authors: Kemal Özbilgin
authors: Department of Otolaryngology-Head & Neck Surgery Osmangazi University, Eskişehir, Turkey.
authors: Cemal
authors: C
authors: Cingi
mesh_terms: D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation; D018424:Cholesteatoma, Middle Ear / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D053612:Janus Kinases / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D015415:Biomarkers; D053612:Janus Kinases