title: Murine esBAF chromatin remodeling complex subunits BAF250a and Brg1 are necessary to maintain and reprogram pluripotency-specific replication timing of select replication domains.
abstract: BACKGROUND
  Cellular differentiation and reprogramming are accompanied by changes in replication timing and 3D organization of large-scale (400 to 800 Kb) chromosomal domains ('replication domains'), but few gene products have been identified whose disruption affects these properties.
  
  
  RESULTS
  Here we show that deletion of esBAF chromatin-remodeling complex components BAF250a and Brg1, but not BAF53a, disrupts replication timing at specific replication domains. Also, BAF250a-deficient fibroblasts reprogrammed to a pluripotency-like state failed to reprogram replication timing in many of these same domains. About half of the replication domains affected by Brg1 loss were also affected by BAF250a loss, but a much larger set of domains was affected by BAF250a loss. esBAF binding in the affected replication domains was dependent upon BAF250a but, most affected domains did not contain genes whose transcription was affected by loss of esBAF.
  
  
  CONCLUSIONS
  Loss of specific esBAF complex subunits alters replication timing of select replication domains in pluripotent cells.
authors: Shin-Ichiro
authors: S
authors: Takebayashi
authors: Ienglam
authors: I
authors: Lei
authors: Tyrone
authors: T
authors: Ryba
authors: Takayo
authors: T
authors: Sasaki
authors: Vishnu
authors: V
authors: Dileep
authors: Dana
authors: D
authors: Battaglia
authors: Xiaolin
authors: X
authors: Gao
authors: Peng
authors: P
authors: Fang
authors: Yong
authors: Y
authors: Fan
authors: Miguel A
authors: MA
authors: Esteban
authors: Jiong
authors: J
authors: Tang
authors: Gerald R
authors: GR
authors: Crabtree
authors: Zhong
authors: Z
authors: Wang
authors: Department of Biological Science, Florida State University, 319 Stadium Drive, Tallahassee, FL, 32306, USA. gilbert@bio.fsu.edu.
authors: David M
authors: DM
authors: Gilbert