title: miR-329 suppresses the growth and motility of neuroblastoma by targeting KDM1A.
abstract: MicroRNAs (miRNAs) act as key regulators of multiple cancers. miR-329 functions as a tumor suppressor in some malignancies. However, its role in neuroblastoma remains poorly understood. We found that miR-329 was decreased in metastatic tumor tissues compared with matched primary tumor tissues. Forced overexpression of miR-329 substantially suppressed cell proliferation, colony formation, migration, and invasion of neuroblastoma cells. Lysine-specific demethylase 1 (KDM1A) was found to be a target of miR-329. Furthermore, down-regulation of KDM1A by shRNA performed similar effects with overexpression of miR-329. Overexpression of KDM1A partially reversed the tumor suppressive effects of miR-329 in neuroblastoma cells. Collectively, miR-329 may suppress neuroblastoma cell growth and motility partially by targeting KDM1A.
authors: Department of Neurosurgery, West Division of Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430056, China.
authors: Haifeng
authors: H
authors: Yang
authors: Department of Neurosurgery, West Division of Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430056, China.
authors: Qi
authors: Q
authors: Li
authors: Department of Neurosurgery, West Division of Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430056, China.
authors: Wohua
authors: W
authors: Zhao
authors: Department of Neurosurgery, West Division of Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430056, China.
authors: Dun
authors: D
authors: Yuan
authors: Department of Neurosurgery, West Division of Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430056, China.
authors: Hongyang
authors: H
authors: Zhao
authors: Department of Neurosurgery, West Division of Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430056, China. Electronic address: zhouyonguh@163.com.
authors: Yong
authors: Y
authors: Zhou
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D001483:Base Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D056466:Histone Demethylases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D009154:Mutation; D009447:Neuroblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D020413:3' Untranslated Regions; C584396:MIRN329 microRNA, human; D035683:MicroRNAs; D008156:Luciferases; D056466:Histone Demethylases; C495195:KDM1A protein, human
keywords: Growth; Invasion; KDM1A; Migration; Neuroblastoma; miR-329