title: In vivo fate of avidin-nucleic acid nanoassemblies as multifunctional diagnostic tools.
abstract: This study describes the formulation optimization and body-cell distribution and clearance in mice of a dually fluorescent biodegradable poly avidin nanoassembly based on the novel Avidin-Nucleic-Acid-Nano-ASsembly (ANANAS) platform as a potential advancement of classic avidin/biotin-based targeted delivery. The nanoformulation circulates freely in the bloodstream; it is slowly captured by filter organs; it is efficiently cleared within 24-48 h, and it is poorly immunogenic. The system displays more favorable properties than its parent monomeric avidin and it is a promising tool for diagnostic purposes for future translational aims, for which free circulation in the bloodstream, safety, multifunctionality and high composition definition are all necessary requirements. In addition, the assembly shows a time-dependent cell penetration capability, suggesting it may also function as a NP-dependent drug delivery tool. The ease of preparation together with the possibility to fine-tune the surface composition makes it also an ideal candidate to understand if and how nanoparticle composition affects its localization.
authors: Department of Biochemistry and Molecular Pharmacology, IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri" , Via La Masa 19, 20156 Milan, Italy.
authors: Paolo
authors: P
authors: Bigini
authors: Sara
authors: S
authors: Previdi
authors: Elisabetta
authors: E
authors: Casarin
authors: Davide
authors: D
authors: Silvestri
authors: Martina Bruna
authors: MB
authors: Violatto
authors: Sonia
authors: S
authors: Facchin
authors: Leopoldo
authors: L
authors: Sitia
authors: Antonio
authors: A
authors: Rosato
authors: Gaia
authors: G
authors: Zuccolotto
authors: Nicola
authors: N
authors: Realdon
authors: Fabio
authors: F
authors: Fiordaliso
authors: Mario
authors: M
authors: Salmona
authors: Margherita
authors: M
authors: Morpurgo
mesh_terms: D000818:Animals; D001360:Avidin / Q000008:administration & dosage*; D051379:Mice; D053758:Nanoparticles; D009696:Nucleic Acids / Q000737:chemistry*
chemical_list: D009696:Nucleic Acids; D001360:Avidin