title: Effects of downregulation of S100A8 protein expression on cell cycle and apoptosis of fibroblasts derived from hypertrophic scars.
abstract: BACKGROUND
  Uncontrolled growth and lack of apoptosis in fibroblasts derived from a hypertrophic scar play an important role in pathology.
  
  
  OBJECTIVE
  The authors explore the contribution of S100A8 overexpression to the phenotype of cells and discuss how the downregulation of S100A8 could inhibit the growth and induce apoptosis of fibroblasts derived from hypertrophic scars.
  
  
  METHODS
  Fibroblasts were harvested from hypertrophic scar tissue in 8 patients treated with small interfering RNA against S100A8 in an in vitro culture. The effects of silencing S100A8 were analyzed by Western blot. Cellular proliferation and apoptosis were detected by flow cytometry.
  
  
  RESULTS
  Fibroblasts treated with small interfering RNA targeting S100A8 showed a significant decrease in S100A8 protein 48 hours after treatment. They also proliferated significantly slower and showed more apoptosis than control fibroblasts.
  
  
  CONCLUSIONS
  Inhibition of S100A8 resulted in significant growth reduction and apoptosis acceleration in fibroblasts derived from hypertrophic scars. Manipulation of S100A8 protein expression by gene silencing may represent something new in the treatment of hypertrophic scarring.
authors: Dr Yuandong is an Associate Professor and Dr Lijun is a Professor in the Department of Plastic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin City, China.
authors: Lv
authors: L
authors: Yaundong
authors: Wang
authors: W
authors: Dongyan
authors: Hao
authors: H
authors: Lijun
authors: Xiao
authors: X
authors: Zhibo
mesh_terms: D000328:Adult; D017209:Apoptosis; D040501:Calgranulin A / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle; D049109:Cell Proliferation; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015536:Down-Regulation; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D010641:Phenotype; D034622:RNA Interference; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors; D014162:Transfection; D055815:Young Adult
chemical_list: D040501:Calgranulin A
keywords: S100A8; apoptosis; fibroblasts; hypertrophic scar; protein expression; research