title: Enzymatic activity profile of a Brazilian culture collection of Candida albicans isolated from diabetics and non-diabetics with oral candidiasis.
abstract: The secretion of hydrolytic enzymes is a fundamental virulence factor of Candida albicans to develop disease. The objective of this study was to characterise the virulence of 148 clinical isolates of C. albicans from oral candidiasis by assessing the expression of phospholipase (PL) and secreted aspartyl proteinase (SAP). Isolates were obtained from healthy subjects (HS) and diabetics (DOC) and non-diabetics with oral candidiasis (NDOC). An aliquot (5 μl) of each cell suspension was inoculated on PL and SAP agar plates and incubated. Enzymes secretion was detected by the formation of an opaque halo around the colonies and enzymatic activity (PZ) was determined by the ratio between colony diameter and colony diameter plus the halo zone. Statistical comparisons were made by a one-way anova followed by Tukey's post hoc test (α = 0.05). The clinical sources of C. albicans had significant effect (P < 0.001) on the PZ values of both enzymes. For PL, clinical isolates from NDOC and DOC had highest enzymatic activity than those from HS (P < 0.05), with no significant differences between them (P = 0.506). For SAP, C. albicans from NDOC showed the lower enzymatic activity (P < 0.001). There were no significant differences between isolates from HS and DOC (P = 0.7051). C. albicans isolates from NDOC and DOC patients showed an increased production of PL.
authors: Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ Estadual Paulista. Rua Humaitá, Araraquara, Sao Paulo, Brazil.
authors: Paula Volpato
authors: PV
authors: Sanitá
authors: Chaiene Evelin
authors: CE
authors: Zago
authors: Ana Cláudia
authors: AC
authors: Pavarina
authors: Janaina Habib
authors: JH
authors: Jorge
authors: Ana Lúcia
authors: AL
authors: Machado
authors: Carlos Eduardo
authors: CE
authors: Vergani
mesh_terms: D057055:Aspartic Acid Proteases / Q000032:analysis*; D001938:Brazil; D002176:Candida albicans / Q000201:enzymology* / Q000302:isolation & purification*; D002180:Candidiasis, Oral / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry; D048909:Diabetes Complications / Q000382:microbiology; D006801:Humans; D008828:Microbiological Techniques; D010740:Phospholipases / Q000032:analysis*; D037521:Virulence Factors / Q000032:analysis*
chemical_list: D003470:Culture Media; D037521:Virulence Factors; D010740:Phospholipases; D057055:Aspartic Acid Proteases
keywords: Candida albicans; diabetes mellitus; oral candidiasis; phospholipase; secreted aspartyl proteinase