title: Host serum miR-223 is a potential new biomarker for Schistosoma japonicum infection and the response to chemotherapy.
abstract: BACKGROUND
  Numerous studies have shown that aberrant microRNA (miRNA) expression is associated with the pathogenesis and progression of various human diseases. Hence, serum miRNAs are considered to be potential biomarkers for the diagnosis of human diseases. This study examined whether several miRNAs known to be commonly deregulated in liver diseases are deregulated in the serum of hosts with hepatic schistosomiasis, and thus whether they could serve as potential markers for detection of schistosome infection and evaluation of the effectiveness of chemotherapy.
  
  
  METHODS
  We analyzed the serum levels of six selected candidate miRNA molecules (miR-146b, miR-122, miR-223, miR-199a-5p, miR-199a-3p, miR-34a) from mice, rabbits, buffalos and humans infected with Schistosoma japonicum using qPCR. We evaluated liver pathology by determining the hydroxyproline content in liver tissues. Primary resident liver cells were isolated to quantify the expression level of deregulated miRNAs. Bioinformatics analyses were also conducted to assess the potential function of miR-223.
  
  
  RESULTS
  Using a mouse model of Schistosoma japonicum infection, we found that the expression level of serum miR-223 was significantly elevated after infection, but returned to near normal levels after the treatment with praziquantel (PZQ). Importantly, the level of serum miR-223 reflected the extent of liver pathology post-infection. We validated the elevated level of the circulating miR-223 in serum samples of other host species including rabbits, buffalos and humans. In addition, our results showed that miR-223 was primarily located in the Kupffer cells, but its expression levels were significantly up-regulated in hepatocytes, hepatic stellate cells and Kupffer cells after infection. Bioinformatics analyses revealed a potential functional role of miR-223 in transcription regulator activity, transcription factor activity and DNA binding.
  
  
  CONCLUSIONS
  This study suggested that the circulating miR-223 could serve as a potential new biomarker for the detection of schistosome infection and the assessment of the response to chemotherapy.
authors: Xing
authors: X
authors: He
authors: Xue
authors: X
authors: Sai
authors: Chao
authors: C
authors: Chen
authors: Yuanbin
authors: Y
authors: Zhang
authors: Xindong
authors: X
authors: Xu
authors: Dongmei
authors: D
authors: Zhang
authors: Weiqing
authors: W
authors: Pan
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D002020:Buffaloes; D005786:Gene Expression Regulation; D006801:Humans; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D011817:Rabbits; D012549:Schistosoma japonicum / Q000502:physiology*; D012554:Schistosomiasis japonica / Q000097:blood* / Q000188:drug therapy / Q000469:parasitology; D012556:Schistosomicides / Q000627:therapeutic use*
chemical_list: D015415:Biomarkers; C544877:MIRN223 microRNA, mouse; D035683:MicroRNAs; D012556:Schistosomicides; D006909:Hydroxyproline