title: Discovery of the rapanone and suberonone mixture as a motif for leishmanicidal and antifungal applications.
abstract: Leishmaniasis and fungal infections are significant diseases impacting worldwide public health. Treatments have developed greatly over time, however, there is a necessity to discover less toxic drugs, which have greater efficacy and are more economically accessible. This work conducted a screening of Cerrado species extracts: Connarus suberosus Planch. (Connaraceae), Neea theifera Oerst. (Nyctaginaceae) and Myrcia linearifolia Cambess. (Myrtaceae) against Leishmania (Leishmania) amazonensis, dermatophytes and yeasts. Leishmanicidal and antifungal tests were conducted using MTT colorimetric assay and CLSI methodology, respectively. Connarus suberosus extracts presented the most promising results against the aforementioned microorganisms, which has not been described in the literature. The root bark EtOAc extract was selected for chemical fractionation resulting in a mixture of rapanone (1) and a previously unreported compound named as suberonone (2); a mixture of β-sitosterol (3) and stigmasterol (4); oleic acid (5); geranilgeraniol (6); and two derivatives obtained from 1 and 2 mixture. The rapanone and suberonone mixture demonstrated a MIC of 15.62 μg/mL against Candida albicans ATCC 10231.
authors: Laboratório de Farmacognosia, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Asa Norte, CEP 70910-900 Brasília, DF, Brazil.
authors: Raphaella Correia
authors: RC
authors: da Costa
authors: Laboratório de Farmacognosia, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Asa Norte, CEP 70910-900 Brasília, DF, Brazil.
authors: Dandara Braga
authors: DB
authors: Santana
authors: Instituto de Química, Universidade Federal do Rio Grande do Norte, Natal, Brazil.
authors: Renata Mendonça
authors: RM
authors: Araújo
authors: Laboratório de Anatomia Vegetal, Universidade de Brasília, Brasília, Brazil.
authors: José Elias
authors: JE
authors: de Paula
authors: Departamento de Química Orgânica, Universidade Federal do Ceará, Fortaleza, Brazil.
authors: Patrícia Coelho
authors: PC
authors: do Nascimento
authors: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil.
authors: Norberto Peporine
authors: NP
authors: Lopes
authors: FAPERJ/Laboratório de Ciências Químicas/UENF/-Departamento de Química, UFRRJ, Seropédica, Brazil.
authors: Raimundo
authors: R
authors: Braz-Filho
authors: Laboratório de Farmacognosia, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Asa Norte, CEP 70910-900 Brasília, DF, Brazil. Electronic address: darvenne@unb.br.
authors: Laila Salmen
authors: LS
authors: Espindola
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D016227:Benzoquinones; D055808:Drug Discovery; D007898:Leishmaniasis, Visceral / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D000935:Antifungal Agents; D016227:Benzoquinones; D008070:Lipopolysaccharides; D010936:Plant Extracts; C054611:rapanone
keywords: Brazilian Cerrado; Candida; Connarus suberosus; Leishmania (Leishmania) amazonensis; Myrcia linearifolia; Neea theifera; Suberonone; Trichophyton