title: miR-200b suppresses cell proliferation, migration and enhances chemosensitivity in prostate cancer by regulating Bmi-1.
abstract: microRNAs (miRNAs) are a class of small non-coding RNAs that can post-transcriptionally regulate gene expression and play critical roles in many important biological processes. The role of miRNAs in prostate cancer (PCa) development and pathogenesis remains largely unknown. In the present study, we showed that miR-200b was downregulated in clinical prostatic tumors when compared to normal prostate tissue and in advanced PCa cell lines when compared to normal epithelial prostatic cells. Enforced miR-200b expression suppressed PCa cell proliferation and migration and enhanced chemosensitivity to docetaxel by targeting B-cell-specific Moloney murine leukemia virus insertion site 1 (Bmi-1). Bmi-1 was detected at higher levels in PCa, and knockdown of Bmi-1 showed similar effects as miR-200b overexpression in PCa cells. Moreover, we confirmed that these effects were correlated with increased levels of E-cadherin and P16 and a reduction in vimentin expression and expression of stem cell markers (CD44 and OCT4). These findings suggest that miR-200b plays vital roles as a tumor-suppressor by targeting Bmi-1 and may be a promising therapeutic target for PCa treatment.
authors: Department of Urology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, P.R. China.
authors: Junjie
authors: J
authors: Yu
authors: Department of Urology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, P.R. China.
authors: Youyi
authors: Y
authors: Lu
authors: Department of Urology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, P.R. China.
authors: Di
authors: D
authors: Cui
authors: Department of Urology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, P.R. China.
authors: Enhui
authors: E
authors: Li
authors: Department of Urology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, P.R. China.
authors: Yipin
authors: Y
authors: Zhu
authors: Department of Urology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, P.R. China.
authors: Yuyang
authors: Y
authors: Zhao
authors: Department of Urology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, P.R. China.
authors: Fujun
authors: F
authors: Zhao
authors: Department of Urology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, P.R. China.
authors: Shujie
authors: S
authors: Xia
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D015820:Cadherins / Q000096:biosynthesis; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000096:biosynthesis; D000077143:Docetaxel; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis; D063150:Polycomb Repressive Complex 1 / Q000096:biosynthesis / Q000235:genetics*; D011467:Prostate / Q000166:cytology; D011471:Prostatic Neoplasms / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering; D043823:Taxoids / Q000494:pharmacology; D025521:Tumor Suppressor Proteins / Q000235:genetics; D014746:Vimentin / Q000096:biosynthesis
chemical_list: D000970:Antineoplastic Agents; C069077:BMI1 protein, human; C497483:CD44 protein, human; D015820:Cadherins; D019941:Cyclin-Dependent Kinase Inhibitor p16; D018960:Hyaluronan Receptors; C533896:MIRN200 microRNA, human; D035683:MicroRNAs; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D034741:RNA, Small Interfering; D043823:Taxoids; D025521:Tumor Suppressor Proteins; D014746:Vimentin; D000077143:Docetaxel; D063150:Polycomb Repressive Complex 1