title: Expression and characterization of a thermostable acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis involved in the degradation of insoluble cellulose acetate.
abstract: A thermostable acetylxylan esterase gene, TTE0866, which catalyzes the deacetylation of cellulose acetate, was cloned from the genome of Caldanaerobacter subterraneus subsp. tengcongensis. The pH and temperature optima were 8.0 and 60 °C. The esterase was inhibited by phenylmethylsulfonyl fluoride. A mixture of the esterase and cellulolytic enzymes efficiently degraded insoluble cellulose acetate with a higher degree of substitution.
authors: Environmental Technology Research Division, Osaka Municipal Technical Research Institute.
authors: Kunihiko
authors: K
authors: Moriyoshi
authors: Daisuke
authors: D
authors: Koma
authors: Hayato
authors: H
authors: Yamanaka
authors: Kiyofumi
authors: K
authors: Sakai
authors: Takashi
authors: T
authors: Ohmoto
mesh_terms: D000115:Acetylesterase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D003001:Cloning, Molecular; D004795:Enzyme Stability; D015870:Gene Expression; D008969:Molecular Sequence Data; D012995:Solubility; D013696:Temperature; D046950:Thermoanaerobacterium / Q000201:enzymology* / Q000235:genetics*
chemical_list: C005062:acetylcellulose; D002482:Cellulose; D000115:Acetylesterase; C081851:acetylxylan esterase