title: Validation of intraluminal and intraperitoneal microdialysis in ischemic small intestine.
abstract: BACKGROUND
  We sought to define the sensitivity and specificity of intraperitoneal (IP) and intraluminal (IL) microdialysate metabolites in depicting ex vivo small intestinal total ischemia during GI-tract surgery. We hypothesized that IL as opposed to IP microdialysis detects small intestinal ischemia with higher sensitivity and specificity.
  
  
  METHODS
  IL and IP microdialysate lactate, pyruvate, glucose and glycerol were analysed from small intestine of pancreaticoduodenectomy patients before and after occluding the mesenteric vasculature and routine resection of a segment of small intestine. Ex vivo time sequences of microdialysate metabolites were described and ROC analyses after 0-30, 31-60, 61-90 and 91-120 minutes after the onset ischemia were calculated.
  
  
  RESULTS
  IL lactate to pyruvate ratio (L/P ratio) indicated ischemia after 31-60 minutes with 0.954 ROC AUC (threshold: 109) in contrast to IP L/P (ROC AUC of 0.938 after 61-90 minutes, threshold: 18). At 31-60 minutes IL glycerol concentration indicated ischemia with 0.903 ROC AUCs (thresholds: 69 μmol/l). IP glycerol was only moderately indicative for ischemia after 91-120 minutes with 0,791 ROC AUCs (threshold 122 μmol/l). After 31-60 minutes IL and IP lactate to glucose ratios (L/G ratio) indicated ischemia with 0.956 and 0,942 ROC AUCs (thresholds: 48,9 and 0.95), respectively.
  
  
  CONCLUSIONS
  The results support the hypothesis that intraluminal application of microdialysis and metabolic parameters from the small intestinal lumen indicate onset of ischemia earlier than intraperioneal microdialysis with higher sensitivity and specificity.
authors: Lauri
authors: L
authors: Pynnönen
authors: Minna
authors: M
authors: Minkkinen
authors: Anders
authors: A
authors: Perner
authors: Sari
authors: S
authors: Räty
authors: Isto
authors: I
authors: Nordback
authors: Juhani
authors: J
authors: Sand
authors: Critical Care Medicine Research Group, Department of Intensive Care Medicine, Tampere University Hospital, Tampere, Finland. jyrki.tenhunen@surgsci.uu.se.
authors: Jyrki
authors: J
authors: Tenhunen
mesh_terms: D000368:Aged; D002277:Carcinoma / Q000601:surgery*; D005260:Female; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006801:Humans; D007421:Intestine, Small / Q000098:blood supply* / Q000378:metabolism; D007511:Ischemia / Q000175:diagnosis* / Q000209:etiology; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D008638:Mesenteric Arteries; D017551:Microdialysis / Q000379:methods*; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000601:surgery*; D016577:Pancreaticoduodenectomy / Q000379:methods*; D011446:Prospective Studies; D019289:Pyruvic Acid / Q000378:metabolism; D012680:Sensitivity and Specificity
chemical_list: D019344:Lactic Acid; D019289:Pyruvic Acid; D005947:Glucose; D005990:Glycerol