title: A multimarker qPCR platform for the characterisation of endometrial cancer.
abstract: The molecular background of endometrial cancer (EC) has not been fully elucidated. In the present study, we developed a quantitative PCR (qPCR) platform to examine the gene dosages of the potential molecular markers MGB1, TOP2A, ERBB1-4, MYC, CCND1, ESR1 and PI3K. The platform was applied in samples collected from 157 EC patients (stage I-IV) to verify its clinical utility and to examine the diagnostic and prognostic significance of the analysed biomarkers. The gene dosage pattern of the ERBB family and its downstream effectors PI3K and MYC showed particularly strong correlations with clinicopathological data. The ERBB PI3K/Akt pathway was upregulated in 31 (20%) of 156 cases. Activation of the ERBB PI3K/Akt pathway was positively correlated with a higher stage (p=0.001), higher grade (p=0.001), histological type II disease (p=0.0003) and metastases (p=0.02). The implemented hierarchical clustering revealed that cluster 2 was characterised by high copy numbers of the studied genes. Cluster 2 was associated with shorter overall survival (p=0.05). The platform was found to be a fast and simple method for direct analysis of the genes involved in uterine carcinogenesis, making it feasible for EC biology characterisation.
authors: Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Dębinki 1, 80-211 Gdańsk, Poland.
authors: Anna
authors: A
authors: Supernat
authors: Department of Gynaecology, Gynaecological Oncology and Gynaecological Endocrinology, Medical University of Gdańsk, Kliniczna 1a, 80-402 Gdańsk, Poland.
authors: Sylwia
authors: S
authors: Łapińska-Szumczyk
authors: Department of Pathomorphology, Medical University of Gdańsk, Dębinki 7, 80-211 Gdańsk, Poland.
authors: Hanna
authors: H
authors: Majewska
authors: Department of Pathology and Neuropathology, Medical University of Gdańsk, Dębinki 1, 80-211 Gdańsk, Poland.
authors: Jacek
authors: J
authors: Gulczyński
authors: Department of Pathomorphology, Medical University of Gdańsk, Dębinki 7, 80-211 Gdańsk, Poland.
authors: Wojciech
authors: W
authors: Biernat
authors: Department of Gynaecology, Gynaecological Oncology and Gynaecological Endocrinology, Medical University of Gdańsk, Kliniczna 1a, 80-402 Gdańsk, Poland.
authors: Dariusz
authors: D
authors: Wydra
authors: Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Dębinki 1, 80-211 Gdańsk, Poland.
authors: Anna J
authors: AJ
authors: Zaczek
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics*; D019938:Cyclin D1 / Q000235:genetics; D004250:DNA Topoisomerases, Type II / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D016889:Endometrial Neoplasms / Q000235:genetics*; D066246:ErbB Receptors / Q000235:genetics; D047628:Estrogen Receptor alpha / Q000235:genetics; D005260:Female; D018628:Gene Dosage / Q000235:genetics*; D006801:Humans; D060785:Mammaglobin A / Q000235:genetics; D008875:Middle Aged; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics; D000075223:Poly-ADP-Ribose Binding Proteins; D016133:Polymerase Chain Reaction; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D019938:Cyclin D1; D004250:DNA Topoisomerases, Type II; D004268:DNA-Binding Proteins; D066246:ErbB Receptors; D047628:Estrogen Receptor alpha; D060785:Mammaglobin A; D019869:Phosphatidylinositol 3-Kinases; D000075223:Poly-ADP-Ribose Binding Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; C530104:CCND1 protein, human; C506487:ESR1 protein, human; C489427:MYC protein, human; C556448:SCGB2A2 protein, human; C000618811:TOP2A protein, human