title: Enantioseparation of racemic trans-δ-viniferin using high speed counter-current chromatography based on induced circular dichroism technology.
abstract: A preparative chiral high speed counter-current chromatography (HSCCC) method based on induced circular dichroism (ICD) spectrum was developed to separate trans-δ-viniferin (TVN) enantiomers successfully. The important parameters for the chiral HSCCC separation process, including the type of chiral selector (CS), the concentration of chiral selector and the equilibrium temperature, were optimized using induced circular dichroism spectrum. The final separation procedure was established with a biphasic solvent system composed of n-hexane-ethyl acetate-25 mmol L(-1) hydroxypropyl-β-cyclodextrin aqueous solution (5:5:10, v/v/v) in the head-to-tail elution mode at 5 °C. Under optimum chiral HSCCC separation conditions, 8.2mg of (7S, 8S)-TVN (1) and 9.4 mg of (7R, 8R)-TVN (2) were successfully separated from 20mg TVN enantiomers with the purity of 99.51% and 99.36%, respectively.
authors: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, Jiangsu, China.
authors: Chao
authors: C
authors: Han
authors: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, Jiangsu, China.
authors: Jinfang
authors: J
authors: Xu
authors: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, Jiangsu, China.
authors: Xiaobing
authors: X
authors: Wang
authors: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, Jiangsu, China.
authors: Xiaoming
authors: X
authors: Xu
authors: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, Jiangsu, China. Electronic address: luojg99@163.com.
authors: Jianguang
authors: J
authors: Luo
authors: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, Jiangsu, China. Electronic address: cpu_lykong@126.com.
authors: Lingyi
authors: L
authors: Kong
mesh_terms: D000073738:2-Hydroxypropyl-beta-cyclodextrin; D001572:Benzofurans / Q000737:chemistry / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods*; D002942:Circular Dichroism / Q000379:methods*; D003377:Countercurrent Distribution / Q000379:methods; D012118:Resorcinols / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism; D013267:Stilbenes / Q000737:chemistry / Q000302:isolation & purification*; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D001572:Benzofurans; D012118:Resorcinols; D013267:Stilbenes; D047392:beta-Cyclodextrins; C477734:delta-viniferin; D000073738:2-Hydroxypropyl-beta-cyclodextrin
keywords: Chiral separation; High speed counter-current chromatography; Hydroxypropyl-β-cyclodextrin; Induced circular dichroism spectrum; Trans-δ-viniferin