title: Lung and serum teicoplanin concentration after aerosol and intravenous administration in a rat model.
abstract: BACKGROUND
  Ventilator-associated pneumonia (VAP) caused by methicillin-resistant Staphylococcus aureus is a major cause of mortality in mechanically ventilated patients. Nebulization of teicoplanin is an alternative way of administration that may provide higher lung tissue concentrations than intravenous (IV) delivery. The aim of this study was to compare the administration of teicoplanin via aerosol with the IV route by measuring the lung and the serum teicoplanin concentrations in a rat model.
  
  
  METHODS
  Eighty healthy male Sprague-Dawley rats were anesthetized and received a single dose of teicoplanin by the IV or aerosol route. After sacrifice, lung and blood samples were collected and teicoplanin concentrations were measured with fluorescence polarization. A noncompartmental approach was used. The area under the concentration curve/minimal inhibition concentration ratio (AUC/MIC), AUC, absorbed fraction, mean residence time (MRT), and mean absorption time (MAT) of teicoplanin were calculated.
  
  
  RESULTS
  Mean±SD lung tissue concentrations of teicoplanin in the aerosol group were significantly higher than those in the IV group (p<0.0001). The mean lung tissue concentration achieved at 15 min was 1.94±1.33 mg/g in the aerosol group and 0.04±0.01 mg/g in the IV group. The mean AUClung was 67.4 mg hr(-1)g(-1) after aerosol and 0.8 mg hr(-1)g(-1) after the IV route. In the aerosol group, AUCserum/MIC ratio was 605/2, and in the IV route, AUCserum/MIC ratio was 682/2. MAT was longer after aerosol than after the IV route (0.91 hr versus 0.06 hr), and MRT was longer after aerosol than after IV bolus administration (6.52 hr versus 5.61 hr).
  
  
  CONCLUSIONS
  Teicoplanin concentrations in the lung tissue of the rat model were significantly higher by the aerosol route than by the IV route. The AUClung after nebulization was 84 times higher than delivery by the IV route, and the AUClung/MIC ratio after nebulization met the recommended target to eradicate Staphylococcus aureus. Administration of teicoplanin by the aerosol route could represent one of the new therapeutic weapons of the treatment of the VAP.
authors: 1 Université François Rabelais , EA 6305, F-37032 Tours, France .
authors: Emmanuelle
authors: E
authors: Mercier
authors: François
authors: F
authors: Darrouzain
authors: Jerome
authors: J
authors: Montharu
authors: Antoine
authors: A
authors: Guillon
authors: Patrice
authors: P
authors: Diot
authors: Gilles
authors: G
authors: Paintaud
authors: Laurent
authors: L
authors: Vecellio
mesh_terms: D000280:Administration, Inhalation; D000336:Aerosols; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*; D019540:Area Under Curve; D007275:Injections, Intravenous; D008168:Lung / Q000378:metabolism*; D008297:Male; D023421:Models, Animal; D053717:Pneumonia, Ventilator-Associated / Q000188:drug therapy / Q000382:microbiology; D017207:Rats, Sprague-Dawley; D017334:Teicoplanin / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*
chemical_list: D000336:Aerosols; D000900:Anti-Bacterial Agents; D017334:Teicoplanin
keywords: antibacterial agents; methicillin-resistant Staphylococcus aureus; nebulization; pneumonia; teicoplanin; ventilator-associated