title: Acri-2,7-Py, a bright red-emitting DNA probe identified through screening of a distyryl dye library.
abstract: The identification of DNA sensors is still a challenge since no DNA probe possesses all the photophysical properties required for live-cell imaging: high fluorescence yield, red emission, permeability, no photobleaching and no cytotoxicity. We describe the preparation of a distyryl dye library and its evaluation on a panel of nucleic acids with various structures (duplex DNA, quadruplex DNA and RNA). The screening involved measuring the modification of the fluorescence properties of the dyes with or without nucleic acids on a microplate reader, and allowed the identification of selective quadruplex DNA ligands with good affinities. Using this screening method we discovered a new bright red-emitting DNA stain, Acri-2,7-Py, for fixed cells. In living cells, the staining was not nuclear and photodamage generated through illumination induced cellular death. These processes require further studies to determine the relevance of Acri-2,7-Py in photodynamic therapy.
authors: Institut Curie, Centre de Recherche, Centre Universitaire, Orsay, France; UMR 176 CNRS, Centre Universitaire, Orsay, France; Université Paris-Sud, Centre Universitaire, Orsay, France.
authors: Delphine
authors: D
authors: Naud-Martin
authors: Xavier
authors: X
authors: Martin-Benlloch
authors: Florent
authors: F
authors: Poyer
authors: Florence
authors: F
authors: Mahuteau-Betzer
authors: Marie-Paule
authors: MP
authors: Teulade-Fichou
mesh_terms: D000166:Acridines / Q000737:chemistry*; D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D019073:HT29 Cells; D057166:High-Throughput Screening Assays; D006801:Humans; D011726:Pyridinium Compounds / Q000737:chemistry*; D054852:Small Molecule Libraries; D013050:Spectrometry, Fluorescence
chemical_list: C000591476:Acri-2.7-Py; D000166:Acridines; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D054852:Small Molecule Libraries; D004247:DNA
keywords: DNA minor-groove binder; DNA stain; Distyryl dye library; Fluorescence-based screening; uadruplex nucleic acids