title: Upregulation of Lhx8 increase VAChT expression and ACh release in neuronal cell line SHSY5Y.
abstract: Lhx8 is a transcription factor for cholinergic differentiation. Our previous experiments found upregulation of Lhx8 promoted cholinergic neuronal differentiation of hippocampal neural stem/progenitor cells or hippocampal newborn neurons in vitro. However, the role of Lhx8 in VAChT expression and ACh release is still less understood. In this report, we transfected Lhx8 cDNA into neuronal cell line SHSY5Y by lentiviral vectors to acquire the cells which stably expressed high level of Lhx8. Using this cell model, we provided experimental evidence that increasing Lhx8 upregulated the expression of ChAT and VAChT, and also increased the ACh release in culture medium. We suggested that Lhx8 overexpression is a useful strategy to increase the release of ACh and maybe of therapeutic value to neurodegenerative diseases.
authors: Department of Human Anatomy, the Jiangsu Key Laboratory of Neuroregeneration, Medical College of Nantong University, Nantong 226001, China.
authors: Haoming
authors: H
authors: Li
authors: Department of Human Anatomy, the Jiangsu Key Laboratory of Neuroregeneration, Medical College of Nantong University, Nantong 226001, China; Department of Human Anatomy and Histoembryology, Medical School of Soochow University, Suzhou 215123, China. Electronic address: jguohua@ntu.edu.cn.
authors: Guohua
authors: G
authors: Jin
authors: Department of Pathology, Shanghai Changzheng Hospital, Shanghai 200003, China.
authors: Peipei
authors: P
authors: Zhu
authors: Department of Human Anatomy, the Jiangsu Key Laboratory of Neuroregeneration, Medical College of Nantong University, Nantong 226001, China; Department of Human Anatomy and Histoembryology, Medical School of Soochow University, Suzhou 215123, China.
authors: Linqing
authors: L
authors: Zou
authors: Department of Human Anatomy, the Jiangsu Key Laboratory of Neuroregeneration, Medical College of Nantong University, Nantong 226001, China; Department of Human Anatomy and Histoembryology, Medical School of Soochow University, Suzhou 215123, China.
authors: Jinhong
authors: J
authors: Shi
authors: Department of Human Anatomy, the Jiangsu Key Laboratory of Neuroregeneration, Medical College of Nantong University, Nantong 226001, China; Department of Human Anatomy and Histoembryology, Medical School of Soochow University, Suzhou 215123, China.
authors: Xin
authors: X
authors: Yi
authors: Department of Human Anatomy, the Jiangsu Key Laboratory of Neuroregeneration, Medical College of Nantong University, Nantong 226001, China.
authors: Xinhua
authors: X
authors: Zhang
authors: Department of Human Anatomy, the Jiangsu Key Laboratory of Neuroregeneration, Medical College of Nantong University, Nantong 226001, China.
authors: Meiling
authors: M
authors: Tian
authors: Department of Human Anatomy, the Jiangsu Key Laboratory of Neuroregeneration, Medical College of Nantong University, Nantong 226001, China.
authors: Jianbing
authors: J
authors: Qin
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation; D006801:Humans; D060850:LIM-Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics*; D009474:Neurons / Q000378:metabolism*; D011897:Random Allocation; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics*; D050494:Vesicular Acetylcholine Transport Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: C112960:LIM homeobox protein 8; D060850:LIM-Homeodomain Proteins; C493459:SLC18A3 protein, human; D014157:Transcription Factors; D050494:Vesicular Acetylcholine Transport Proteins; D000109:Acetylcholine
keywords: ACh; ChAT; Lhx8; SHSY5Y; VAChT