title: Photodynamic therapy of Porphyromonas gingivalis via liposome-encapsulated sensitizers.
abstract: Photodynamic therapy exploits the light-activation of a photosensitizer to cause cytotoxicity. Liposomes can be used to deliver hydrophobic photosensitizers to bacteria. Positively charged dioleoyltrimethylammoniumpropane:palmitoyloleoylphosphatidylcholine (1:1) liposomes bound quantitatively to the periodontal pathogen, Porphyromonas gingivalis. Following illumination, free and liposomal zinc phthalocyanine reduced the colony-forming unit (CFU) to 65 percent and 23 percent of controls, respectively. Thus, localization of the photosensitizer at the surface of bacteria via liposome binding enhanced the photodynamic cytotoxicity of zinc phthalocyanine.
authors: University of the Pacific, Arthur A. Dugoni School of Dentistry in San Francisco, USA. a_ko1@u.pacific.edu
authors: Alex
authors: A
authors: Ko
authors: Microbiology Research Laboratory, University of the Pacific, Arthur A.Dugoni School of Dentistry in San Francisco, USA.
authors: Michael
authors: M
authors: Yee
authors: Poznan University of Medical Sciences, Poznan, Poland.
authors: Paulina
authors: P
authors: Skupin-Mrugalska
authors: Department of Microbiology, University of the Pacific, Arthur A. Dugoni School of Dentistry in San Francisco, USA.
authors: Nejat
authors: N
authors: Düzgünes
mesh_terms: D001422:Bacterial Adhesion; D015169:Colony Count, Microbial; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D054833:Isoindoles; D008081:Liposomes / Q000737:chemistry*; D008565:Membrane Proteins; D009942:Organometallic Compounds / Q000494:pharmacology*; D010518:Periodontitis / Q000188:drug therapy / Q000382:microbiology; D010713:Phosphatidylcholines / Q000494:pharmacology; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000494:pharmacology*; D016966:Porphyromonas gingivalis / Q000187:drug effects*; D011485:Protein Binding; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D017967:Zinc Compounds
chemical_list: D005229:Fatty Acids, Monounsaturated; D007211:Indoles; D054833:Isoindoles; D008081:Liposomes; D008565:Membrane Proteins; D009942:Organometallic Compounds; D010713:Phosphatidylcholines; D017319:Photosensitizing Agents; D000644:Quaternary Ammonium Compounds; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane; C028694:1-palmitoyl-2-oleoylphosphatidylcholine