title: Transcriptional activity of the FUT1 gene promoter region in pigs.
abstract: This study aims to provide a theoretical basis on the regulatory mechanism of the α-l,2-fucosyltransferase (FUT1) gene in pigs by analyzing the transcriptional activity of its promoter region. On the basis of the previously obtained promoter sequence, primers upstream and downstream of the gene were designed using the restriction endonucleases KpnI and HindIII respectively, and the recombinant plasmids of the pGL3-promoter were constructed by inserting promoter sequences with partially missing regions. The resultant mutants were observed by transient transfection assay into HEK293 cells, and the transcriptional activity of the promoter region was determined by luciferase activity. The 5'-flanking region of the FUT1 gene (-1150 to +50 bp) exhibited promoter activity. The -1150-bp to -849-bp region showed negative regulation of the gene. The recombinant plasmid pGL3-898 showed the strongest luciferase activity, and the activity showed a decreasing trend when the deleted region was increased. Recombinant plasmids were successfully constructed, verified, and the positive and negative regulation areas and core promoter region were detected, providing a deeper insight into the transcriptional regulatory mechanism of the FUT1 gene.
authors: Chen
authors: C
authors: Zi
authors: Zhengchang
authors: Z
authors: Wu
authors: Jing
authors: J
authors: Wang
authors: Yongjiu
authors: Y
authors: Huo
authors: Guoqiang
authors: G
authors: Zhu
authors: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China. slwu@yzu.edu.cn.
authors: Shenglong
authors: S
authors: Wu
authors: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China. wbbao@yzu.edu.cn.
authors: Wenbin
authors: W
authors: Bao
mesh_terms: D000818:Animals; D005647:Fucosyltransferases / Q000235:genetics* / Q000378:metabolism*; D017930:Genes, Reporter; D057809:HEK293 Cells; D006801:Humans; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D013552:Swine; D014158:Transcription, Genetic; D014162:Transfection; D000097631:Galactoside 2-alpha-L-fucosyltransferase
chemical_list: D005647:Fucosyltransferases; D000097631:Galactoside 2-alpha-L-fucosyltransferase