title: Overlapping chromatin-remodeling systems collaborate genome wide at dynamic chromatin transitions.
abstract: ATP-dependent chromatin remodeling is an essential process required for the dynamic organization of chromatin structure. Here we describe the genome-wide location and activity of three remodeler proteins with diverse physiological functions in the mouse genome: Brg1, Chd4 and Snf2h. The localization patterns of all three proteins substantially overlap with one another and with regions of accessible chromatin. Furthermore, using inducible mutant variants, we demonstrate that the catalytic activity of these proteins contributes to the remodeling of chromatin genome wide and that each of these remodelers can independently regulate chromatin reorganization at distinct sites. Many regions require the activity of more than one remodeler to regulate accessibility. These findings provide a dynamic view of chromatin organization and highlight the differential contributions of remodelers to chromatin maintenance in higher eukaryotes.
authors: Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors: Stephanie A
authors: SA
authors: Morris
authors: Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors: Songjoon
authors: S
authors: Baek
authors: Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors: Myong-Hee
authors: MH
authors: Sung
authors: Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors: Sam
authors: S
authors: John
authors: Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors: Malgorzata
authors: M
authors: Wiench
authors: Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors: Thomas A
authors: TA
authors: Johnson
authors: Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors: R Louis
authors: RL
authors: Schiltz
authors: Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors: Gordon L
authors: GL
authors: Hager
mesh_terms: D000818:Animals; D001665:Binding Sites; D042002:Chromatin Assembly and Disassembly; D016678:Genome; D051379:Mice