title: New experimental model of exposure to environmental tobacco smoke.
abstract: OBJECTIVE
  To describe a new model to passive smoking for rodents.
  
  
  METHODS
  Twenty rats were distributed into two study groups (N=10): control group (CG), that was not exposed to tobacco smoke and used as normal standard for biochemical and histological analysis; Experimental Group (EG), that Animals were exposed to the passive smoking; Euthanasia was performed after 14 days of exposure. The serum level of nicotine and histological analysis were performed.
  
  
  RESULTS
  There was a statistical difference on the nicotine serum levels between Experimental and Control group, with level of 286 ± 23 nanograma/mL in the EG and undetectable on CG (p<0.01). The histological study suggested the model efficacy producing alveolar destruction and emphysema in the EG compared with the insignificant lesions in the CG's lung.
  
  
  CONCLUSIONS
  The model of exposure to environmental tobacco smoke for rodents induced easily the changes related to secondhand smoke.
authors: Marcus Vinicius Henriques
authors: MV
authors: Brito
authors: Edson Yuzur
authors: EY
authors: Yasojima
authors: Edvaldo Lima
authors: EL
authors: Silveira
authors: Vitor Nagai
authors: VN
authors: Yamaki
authors: Renan Kleber Costa
authors: RK
authors: Teixeira
authors: Daniel Haber
authors: DH
authors: Feijó
authors: Thiago Barbosa
authors: TB
authors: Gonçalves
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004781:Environmental Exposure; D004867:Equipment Design; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008297:Male; D009538:Nicotine / Q000097:blood; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012907:Smoking / Q000009:adverse effects; D013997:Time Factors; D014028:Tobacco Smoke Pollution
chemical_list: D014028:Tobacco Smoke Pollution; D009538:Nicotine