title: Preparation and characterization of mTHPC-loaded solid lipid nanoparticles for photodynamic therapy.
abstract: Among various attempts to enhance the therapeutic efficacy of photodynamic therapy (PDT), the specific delivery of photosensitizer (PS) in the tumor tissue is expected to improve its clinical applications. The aim of this study was to engineer lipid nanoparticles (LNP) with different sizes and various PS contents, using simple solvent-free and easily scale up manufacturing processes. Meso-(tetrahydroxyphenyl) chlorin (mTHPC) is one of the most potent photoactive compounds for clinical use. We demonstrated that mTHPC was efficiently incorporated into the lipid core of LNP, leading to a large range of stable and reproducible mTHPC-loaded LNP with narrow size distribution. Photophysical and physico-chemical properties of mTHPC-loaded LNP were assessed as well as absorption spectra and singlet oxygen emission, colloidal stability, particle size and zeta potential. The photocytotoxicity of selected mTHPC-loaded solid LNP was demonstrated on MCF-7 cells under irradiation at 652nm with a range of light fluence from 1.0 to 10J/cm(2). All physico-chemical, photophysical and biological results allow us to conclude that solid LNP appear as a very promising nano-mTHPC delivery system for PDT.
authors: CEA, LETI, Campus MINATEC, Technologies for Biology and Health Division, 17 avenue des Martyrs, Grenoble Cedex 9 38054, France.
authors: Fabrice P
authors: FP
authors: Navarro
authors: Université de Lorraine, CRAN, UMR 7039, Campus Sciences, BP 70239, Vandœuvre-lès-Nancy 54506, France; CNRS, CRAN, UMR 7039, Vandœuvre-lès-Nancy, France.
authors: Gaëlle
authors: G
authors: Creusat
authors: CNRS, LRGP, UMR 3349, 1 rue Granville BP 20451, 54001 Nancy, France; GdR CNRS 3049 "Médicaments Photoactivables-Photochimiothérapie (PHOTOMED)", France.
authors: Céline
authors: C
authors: Frochot
authors: CNRS, LRGP, UMR 3349, 1 rue Granville BP 20451, 54001 Nancy, France.
authors: Albert
authors: A
authors: Moussaron
authors: CNRS, LRGP, UMR 3349, 1 rue Granville BP 20451, 54001 Nancy, France.
authors: Marc
authors: M
authors: Verhille
authors: Université de Lorraine, CRAN, UMR 7039, Campus Sciences, BP 70239, Vandœuvre-lès-Nancy 54506, France; CNRS, LCPM, FRE 7568, 1 rue Granville BP 20451, 54001 Nancy, France.
authors: Régis
authors: R
authors: Vanderesse
authors: CEA, LETI, Campus MINATEC, Technologies for Biology and Health Division, 17 avenue des Martyrs, Grenoble Cedex 9 38054, France.
authors: Jean-Sébastien
authors: JS
authors: Thomann
authors: CEA, LETI, Campus MINATEC, Technologies for Biology and Health Division, 17 avenue des Martyrs, Grenoble Cedex 9 38054, France.
authors: Patrick
authors: P
authors: Boisseau
authors: CEA, LETI, Campus MINATEC, Technologies for Biology and Health Division, 17 avenue des Martyrs, Grenoble Cedex 9 38054, France.
authors: Isabelle
authors: I
authors: Texier
authors: CEA, LETI, Campus MINATEC, Technologies for Biology and Health Division, 17 avenue des Martyrs, Grenoble Cedex 9 38054, France.
authors: Anne-Claude
authors: AC
authors: Couffin
authors: Université de Lorraine, CRAN, UMR 7039, Campus Sciences, BP 70239, Vandœuvre-lès-Nancy 54506, France; CNRS, CRAN, UMR 7039, Vandœuvre-lès-Nancy, France; GdR CNRS 3049 "Médicaments Photoactivables-Photochimiothérapie (PHOTOMED)", France. Electronic address: muriel.barberi@univ-lorraine.fr.
authors: Muriel
authors: M
authors: Barberi-Heyob
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry*; D004339:Drug Compounding; D006801:Humans; D008027:Light; D008055:Lipids / Q000737:chemistry*; D061986:MCF-7 Cells; D008652:Mesoporphyrins / Q000008:administration & dosage / Q000737:chemistry*; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000737:chemistry*; D026082:Singlet Oxygen / Q000737:chemistry
chemical_list: D004337:Drug Carriers; D008055:Lipids; D008652:Mesoporphyrins; D017319:Photosensitizing Agents; D026082:Singlet Oxygen; C072269:temoporfin
keywords: Drug delivery system; In vitro photocytoxicity; Lipid nanoparticle; Photodynamic therapy; mTHPC