title: Evaluation of FTIR spectroscopy as diagnostic tool for colorectal cancer using spectral analysis.
abstract: The aim of this study is to confirm FTIR spectroscopy as a diagnostic tool for colorectal cancer. 180 freshly removed colorectal samples were collected from 90 patients for spectrum analysis. The ratios of spectral intensity and relative intensity (/I1460) were calculated. Principal component analysis (PCA) and Fisher's discriminant analysis (FDA) were applied to distinguish the malignant from normal. The FTIR parameters of colorectal cancer and normal tissues were distinguished due to the contents or configurations of nucleic acids, proteins, lipids and carbohydrates. Related to nitrogen containing, water, protein and nucleic acid were increased significantly in the malignant group. Six parameters were selected as independent factors to perform discriminant functions. The sensitivity for FTIR in diagnosing colorectal cancer was 96.6% by discriminant analysis. Our study demonstrates that FTIR can be a useful technique for detection of colorectal cancer and may be applied in clinical colorectal cancer diagnosis.
authors: Department of General Surgery, First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
authors: Liu
authors: L
authors: Dong
authors: Department of General Surgery, First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi, China. Electronic address: sunxy@mail.xjtu.edu.cn.
authors: Xuejun
authors: X
authors: Sun
authors: Department of General Surgery, First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
authors: Zhang
authors: Z
authors: Chao
authors: Department of General Surgery, First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
authors: Shiyun
authors: S
authors: Zhang
authors: Department of General Surgery, First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
authors: Jianbao
authors: J
authors: Zheng
authors: Department of General Surgery, First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
authors: Rajendra
authors: R
authors: Gurung
authors: Department of General Surgery, First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
authors: Junkai
authors: J
authors: Du
authors: Department of General Surgery, First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
authors: Jingsen
authors: J
authors: Shi
authors: College of Chemistry and Molecular Engineering, Peking University, Beijing, China.
authors: Yizhuang
authors: Y
authors: Xu
authors: College of Chemistry and Molecular Engineering, Peking University, Beijing, China.
authors: Yuanfu
authors: Y
authors: Zhang
authors: College of Chemistry and Molecular Engineering, Peking University, Beijing, China.
authors: Jinguang
authors: J
authors: Wu
mesh_terms: D000328:Adult; D000368:Aged; D003106:Colon / Q000473:pathology; D015179:Colorectal Neoplasms / Q000175:diagnosis*; D016002:Discriminant Analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D025341:Principal Component Analysis; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
keywords: Colorectal cancerl; FTIR spectroscopy; Principal component analysis; Spectrum