title: PGC7 suppresses TET3 for protecting DNA methylation.
abstract: Ten-eleven translocation (TET) family enzymes convert 5-methylcytosine to 5-hydroxylmethylcytosine. However, the molecular mechanism that regulates this biological process is not clear. Here, we show the evidence that PGC7 (also known as Dppa3 or Stella) interacts with TET2 and TET3 both in vitro and in vivo to suppress the enzymatic activity of TET2 and TET3. Moreover, lacking PGC7 induces the loss of DNA methylation at imprinting loci. Genome-wide analysis of PGC7 reveals a consensus DNA motif that is recognized by PGC7. The CpG islands surrounding the PGC7-binding motifs are hypermethylated. Taken together, our study demonstrates a molecular mechanism by which PGC7 protects DNA methylation from TET family enzyme-dependent oxidation.
authors: Department of Internal Medicine, Division of Molecular Medicine and Genetics, University of Michigan Medical School, 1150 W. Medical Center Drive, 5560 MSRBII, Ann Arbor, MI 48109, USA.
authors: Chunjing
authors: C
authors: Bian
authors: Xiaochun
authors: X
authors: Yu
mesh_terms: D002460:Cell Line; D002868:Chromosomal Proteins, Non-Histone; D019175:DNA Methylation; D004268:DNA-Binding Proteins / Q000378:metabolism; D049308:Dioxygenases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; C466105:DPPA3 protein, human; D011506:Proteins; D011518:Proto-Oncogene Proteins; C578792:TET3 protein, human; D049308:Dioxygenases; C541081:TET2 protein, human