title: Characterization of cardiolipins and their oxidation products by LC-MS analysis.
abstract: Cardiolipins, a class of mitochondria-specific lipid molecules, is one of the most unusual and ancient phospholipids found in essentially all living species. Typical of mammalian cells is the presence of vulnerable to oxidation polyunsaturated fatty acid resides in CL molecules. The overall role and involvement of cardiolipin oxidation (CLox) products in major intracellular signaling as well as extracellular inflammatory and immune responses have been established. However, identification of individual peroxidized molecular species in the context of their ability to induce specific biological responses has not been yet achieved. This is due, at least in part, to technological difficulties in detection, identification, structural characterization and quantitation of CLox associated with their very low abundance and exquisite diversification. This dictates the need for the development of new methodologies for reliable, sensitive and selective analysis of both CLox. LC-MS-based oxidative lipidomics with high mass accuracy instrumentation as well as new software packages are promising in achieving the goals of expedited and reliable analysis of cardiolipin oxygenated species in biosamples.
authors: Center for Free Radical and Antioxidant Health, University of Pittsburgh, Pittsburgh, PA 15219, USA; Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA 15219, USA. Electronic address: yyt1@pitt.edu.
authors: Yulia Y
authors: YY
authors: Tyurina
authors: Mass Spectrometry Center, University of Aveiro, 3810-193 Aveiro, Portugal; QOPNA, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
authors: Rosario M
authors: RM
authors: Domingues
authors: Center for Free Radical and Antioxidant Health, University of Pittsburgh, Pittsburgh, PA 15219, USA; Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA 15219, USA.
authors: Vladimir A
authors: VA
authors: Tyurin
authors: Mass Spectrometry Center, University of Aveiro, 3810-193 Aveiro, Portugal; QOPNA, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
authors: Elisabete
authors: E
authors: Maciel
authors: Mass Spectrometry Center, University of Aveiro, 3810-193 Aveiro, Portugal; QOPNA, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
authors: Pedro
authors: P
authors: Domingues
authors: Center for Free Radical and Antioxidant Health, University of Pittsburgh, Pittsburgh, PA 15219, USA; Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA 15219, USA.
authors: Andrew A
authors: AA
authors: Amoscato
authors: Center for Free Radical and Antioxidant Health, University of Pittsburgh, Pittsburgh, PA 15219, USA; Department of Critical Care Medicine, University of Pittsburgh, Pittsburgh, PA 15219, USA; Safar Center for Resuscitation Research, University of Pittsburgh, Pittsburgh, PA 15219, USA.
authors: Hülya
authors: H
authors: Bayir
authors: Center for Free Radical and Antioxidant Health, University of Pittsburgh, Pittsburgh, PA 15219, USA; Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA 15219, USA.
authors: Valerian E
authors: VE
authors: Kagan
mesh_terms: D000818:Animals; D002308:Cardiolipins / Q000737:chemistry / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods*; D019295:Computational Biology; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D010084:Oxidation-Reduction
chemical_list: D002308:Cardiolipins
keywords: Cardiolipin; Lipidomics; Mass spectrometry; Oxidative lipidomics; Oxidized cardiolipin