title: On artefact-free reconstruction of low-energy (30-250eV) electron holograms.
abstract: Low-energy electrons (30-250eV) have been successfully employed for imaging individual biomolecules. The most simple and elegant design of a low-energy electron microscope for imaging biomolecules is a lensless setup that operates in the holographic mode. In this work we address the problem associated with the reconstruction from the recorded holograms. We discuss the twin image problem intrinsic to inline holography and the problem of the so-called biprism-like effect specific to low-energy electrons. We demonstrate how the presence of the biprism-like effect can be efficiently identified and circumvented. The presented sideband filtering reconstruction method eliminates the twin image and allows for reconstruction despite the biprism-like effect, which we demonstrate on both, simulated and experimental examples.
authors: Physics Institute, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland. Electronic address: tatiana@physik.uzh.ch.
authors: Tatiana
authors: T
authors: Latychevskaia
authors: Physics Institute, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.
authors: Jean-Nicolas
authors: JN
authors: Longchamp
authors: Physics Institute, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.
authors: Conrad
authors: C
authors: Escher
authors: Physics Institute, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.
authors: Hans-Werner
authors: HW
authors: Fink
mesh_terms: D016477:Artifacts; D004583:Electrons; D006696:Holography / Q000379:methods* / Q000706:statistics & numerical data; D007091:Image Processing, Computer-Assisted / Q000379:methods / Q000706:statistics & numerical data
keywords: Biomolecules; Holography; Imaging; Low-energy electrons; Reconstruction