title: Characterization of galectin-1-positive cells in the mouse hippocampus.
abstract: Galectin-1 (gal-1) is one of several well-studied proteins from the galectin families. It is a 14.5 kDa glycoprotein with a single carbohydrate-binding domain. To examine the distribution and properties of gal-1 in the mouse hippocampus, we performed immunohistochemistry using an anti-gal-1 antibody. We found that most gal-1-positive cells showed both NeuN and β-tubulin III (Tuj-1) immunoreactivity (NeuN: 93%, β-tubulin III: 88%). Furthermore, we clarified that 77% of gal-1-positive cells expressed somatostatin, 79% of gal-1-positive cells expressed GAD67, 34% of gal-1-positive cells expressed parvalbumin, 5% of gal-1-positive cells expressed calretinin, 2% of gal-1-positive cells expressed calbindin, and 31% of gal-1-positive cells expressed neuropeptide Y in the mouse hippocampus. These results indicate that gal-1 is expressed in interneurons that also express β-tubulin III and gal-1 may be a novel marker for interneuron subpopulations in the hippocampus.
authors: aDivision of Neurofunctional Genomics, Medical Institute of Bioregulation bDepartment of Neuropsychiatry, Graduate School of Medical Sciences, Kyushu University, Fukuoka cDepartment of Biotechnology, Maebashi Institute of Technology, Maebashi, Gunma dTechnoMaster Co. Ltd, Yokohama, Japan.
authors: Kosuke
authors: K
authors: Kajitani
authors: Yuko
authors: Y
authors: Kobayakawa
authors: Hiroko
authors: H
authors: Nomaru
authors: Toshihiko
authors: T
authors: Kadoya
authors: Hidenori
authors: H
authors: Horie
authors: Yusaku
authors: Y
authors: Nakabeppu
mesh_terms: D000818:Animals; D064032:Calbindin 2 / Q000378:metabolism; D004268:DNA-Binding Proteins; D037483:Galectin 1 / Q000378:metabolism*; D005968:Glutamate Decarboxylase / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism*; D007150:Immunohistochemistry; D007395:Interneurons / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009478:Neuropeptide Y / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D010320:Parvalbumins / Q000378:metabolism; D013004:Somatostatin / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
chemical_list: C576511:Calb2 protein, mouse; D064032:Calbindin 2; D004268:DNA-Binding Proteins; D037483:Galectin 1; D009419:Nerve Tissue Proteins; C492380:NeuN protein, mouse; D009478:Neuropeptide Y; D009687:Nuclear Proteins; D010320:Parvalbumins; D014404:Tubulin; C462941:beta3 tubulin, mouse; D013004:Somatostatin; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1