title: [Subcellular localization of severe fever with thrombocytopenia syndrome virus in macrophages].
abstract: OBJECTIVE
  To study the subcellular localization of severe fever with thrombocytopenia syndrome virus (SFTSV) in macrophages and understand the replication and assembly mechanism of SFTSV in host cells.
  
  
  METHODS
  Using two types of human macrophage cell lines THP-1 and U937, the study analyzed the intracellular colocalization of SFTSV with Golgi apparatus and endoplasmic reticulum by immunefluorescence staining and confocal microscopy.
  
  
  RESULTS
  SFTSV infected macrophage cell lines THP-1 and U937. Immunofluorescence staining showed that the SFTSV nuclear protein colocalized with Golgi apparatus and closely surrounded by endoplasmic reticulum in the perinuclear region.
  
  
  CONCLUSIONS
  The results suggested that Golgi complex and endoplasmic reticulum are probably the sites for formation and maturation of SFTSV viral particles.
authors: Wenzhou Medical University, Wenzhou, China.
authors: Ying
authors: Y
authors: Han
authors: Yi-Xiang
authors: YX
authors: Li
authors: Cong
authors: C
authors: Jin
authors: Chuan
authors: C
authors: Li
authors: Jing
authors: J
authors: Qu
authors: Fu-Shun
authors: FS
authors: Zhang
authors: Quan-Fu
authors: QF
authors: Zhang
authors: Mi-Fang
authors: MF
authors: Liang
authors: Pei-Hong
authors: PH
authors: Qiu
mesh_terms: D002043:Bunyaviridae / Q000302:isolation & purification*; D045744:Cell Line, Tumor; D004721:Endoplasmic Reticulum / Q000821:virology; D005334:Fever / Q000821:virology*; D006056:Golgi Apparatus / Q000821:virology; D006801:Humans; D008264:Macrophages / Q000821:virology*; D013921:Thrombocytopenia / Q000821:virology*