title: Ridostin induces transcription of a wide spectrum of interferon genes in human cells.
abstract: The effects of Ridostin on the transcription of IFN family genes in human fibroblasts and lymphocytes were studied by quantitative real-time PCR. The degree of gene induction by Ridostin was most pronounced in fibroblasts, and was significantly higher than the induction by Kagocel: transcription of IFN-β, oligoadenylate synthetase, and double-stranded RNA-dependent protein kinase genes increased by about 2000, 100, and 20 times, respectively. In lymphocytes, Ridostin also activated a wide variety of IFN family genes, including genes of IFN-β, IFN-γ, and IFN-dependent enzymes, but this induction was less pronounced than in the fibroblasts. It was shown that gene response in lymphocyte from a child with cancer is reduced in comparison with that of adult healthy participant. Ridostin, and even more so Reaferon up-regulated activities of β-actin, glycerophosphate dehydrogenase, and β2-microglobulin genes, thus making impossible or limiting their use as constitutive stable reference genes (standards) in PCR-assays of IFN and their inductors.
authors: N. F. Gamalea Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation; D. I. Ivanoskii Institute of Virology, Ministry of Health of the Russian Federation, Moscow, Russia. tmsokolovavir@mail.ru.
authors: T M
authors: TM
authors: Sokolova
authors: A N
authors: AN
authors: Shuvalov
authors: M V
authors: MV
authors: Telkov
authors: L V
authors: LV
authors: Kolodyazhnaya
authors: F I
authors: FI
authors: Ershov
mesh_terms: D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis / Q000235:genetics; D000199:Actins / Q000096:biosynthesis / Q000235:genetics; D000328:Adult; D000998:Antiviral Agents / Q000494:pharmacology; D002460:Cell Line; D002648:Child; D005347:Fibroblasts / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics; D006072:Gossypol / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology*; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology; D016899:Interferon-beta / Q000096:biosynthesis / Q000235:genetics; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D007372:Interferons / Q000096:biosynthesis* / Q000235:genetics; D008214:Lymphocytes / Q000378:metabolism; D004680:Maus Elberfeld virus / Q000187:drug effects; D012330:RNA, Double-Stranded / Q000494:pharmacology*; D012331:RNA, Fungal / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects*; D015533:Transcriptional Activation / Q000187:drug effects*; D001613:beta 2-Microglobulin / Q000096:biosynthesis / Q000235:genetics; D019892:eIF-2 Kinase / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000199:Actins; D000998:Antiviral Agents; D007369:Interferon Inducers; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D012330:RNA, Double-Stranded; D012331:RNA, Fungal; D011994:Recombinant Proteins; D001613:beta 2-Microglobulin; C076741:ridostin; C056991:cagocel-1; D016899:Interferon-beta; D007371:Interferon-gamma; D007372:Interferons; D005993:Glycerolphosphate Dehydrogenase; D019892:eIF-2 Kinase; D015088:2',5'-Oligoadenylate Synthetase; D006072:Gossypol