title: Molecular characterization of a novel victorivirus from the entomopathogenic fungus Beauveria bassiana.
abstract: New Zealand isolates of the entomopathogenic fungus Beauveria were examined for the presence of dsRNAs and virus-like particles. Seven out of nine isolates contained one or more high-molecular-weight dsRNAs and all seven contained isometric virus particles ranging in size from 30 to 50 nm. B. bassiana isolate ICMP#6887 contained a single dsRNA band of ~6 kb and isometric virus-like particles of ~50 nm in diameter. Sequencing revealed that the virus from ICMP#6887 had a genome of 5,327 nt with two overlapping ORFs coding for a putative coat protein (CP) and an RNA-dependent RNA-polymerase (RdRp). The sequence showed a highest CP identity of 58.3 % to Tolypocladium cylindrosporum virus 1 (TcV1) and a highest RdRp identity of 48.8 % to Sphaeropsis sapinea RNA virus 1 (SsRV1). Since both TcV1 and SsRV1 belong to the genus Victorivirus, the new virus from B. bassiana ICMP#6887 was tentatively assigned the name Beauveria bassiana victorivirus 1 (BbVV1-6887).
authors: Department of Molecular Bioscience, College of Biomedical Science, Kang Won National University, Hyoja Dong, Chun Cheon Si, Kangwon Do, Korea.
authors: Se Won
authors: SW
authors: Yie
authors: Mahmoud E
authors: ME
authors: Khalifa
authors: Torque
authors: T
authors: Hahn
authors: Michael N
authors: MN
authors: Pearson
mesh_terms: D000818:Animals; D052982:Beauveria / Q000821:virology*; D016000:Cluster Analysis; D016679:Genome, Viral; D009520:New Zealand; D016366:Open Reading Frames; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D019196:Totiviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins