title: A triple staining method for accurate cell cycle analysis using multiparameter flow cytometry.
abstract: Cell cycle analysis is important for cancer research. We present herein a novel method for accurate cell cycle analysis. This method analyzes the cell cycle by multiparameter flow cytometry based on simultaneously labeling the cell nuclear DNA, RNA, and phosphorylated mitotic nuclei protein, using Hoechst 33342, pyronin Y, and MPM-2-Cy5, respectively, and our results demonstrated that this method could effectively divide the cell cycle into G0, G1, S, G2, and M phases. We further tested this method using the clinical anticancer agents crizotinib and taxol, and the results clearly illustrated that crizotinib and taxol arrested Jurkat cells in G0 and M phase, respectively. These results indicate that this method could be a very useful tool for cytokinetic and pharmacological research.
authors: Lin
authors: L
authors: Qiu
authors: Institute for Nutritional Sciences, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. lmouc@hotmail.com.
authors: Ming
authors: M
authors: Liu
authors: Institute for Nutritional Sciences, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. klpan@sibs.ac.cn.
authors: Konglun
authors: K
authors: Pan
mesh_terms: D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology*; D059447:Cell Cycle Checkpoints / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D000077547:Crizotinib; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D019169:Jurkat Cells; D017239:Paclitaxel / Q000494:pharmacology; D011720:Pyrazoles / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology
chemical_list: D011720:Pyrazoles; D011725:Pyridines; D000077547:Crizotinib; D004247:DNA; D017239:Paclitaxel