title: Endothelial progenitor cells are influenced by serum of patients with systemic inflammatory response syndrome or multiple organ dysfunction.
abstract: OBJECTIVE
  To investigate the effects of systemic inflammatory response syndrome (SIRS) and multiple organ dysfunction syndromes (MODS) on human peripheral blood endothelial progenitor cells.
  
  
  METHODS
  Twenty patients admitted to Changhai Hospital, Second Military Medical University, from February, 2011 to November, 2011 were recruited consecutively. The serum samples were collected from the twenty patients who were divided into four groups as following: normal group, post-traumatic group without SIRS, post-traumatic group with SIRS, and post-traumatic group with MODS. Endothelial progenitor cells (EPCs) were isolated from peripheral blood of healthy subjects by using density gradient centrifugation and the effect of the serum on EPCs was detected after stimulating by the serum samples for 0, 6, 12, 24, and 36 h.
  
  
  RESULTS
  Compared with the normal group, the proliferation of EPCs was significantly increased in a time-independent manner in the other three groups, especially in the SIRS serum treated group. The expression of pro-inflammation cytokines was increased in the other three groups compared with the normal group, but the expression of IL-10 in the normal group was higher than the other groups.
  
  
  CONCLUSIONS
  Oxidative stress balance was also broken as the disease progressed. Serum from patients with sepsis could influence proliferation and the inflammation and oxidative stress states of EPCs.
authors: Department of General Surgery, Changhai Hospital, The Second Military Medical University, Shanghai, China. guoenfangggeeff@hotmail.com  
authors: T
authors: T
authors: Pang
authors: W
authors: W
authors: Chen
authors: Z-M
authors: ZM
authors: Lu
authors: T-H
authors: TH
authors: Luo
authors: H
authors: H
authors: Zhou
authors: X-C
authors: XC
authors: Xue
authors: J-W
authors: JW
authors: Bi
authors: G-E
authors: GE
authors: Fang
mesh_terms: D002465:Cell Movement; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D060833:Cellular Microenvironment; D002681:China; D016207:Cytokines / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D009102:Multiple Organ Failure / Q000097:blood*; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism; D018919:Neovascularization, Physiologic; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism* / Q000473:pathology; D018746:Systemic Inflammatory Response Syndrome / Q000097:blood*; D013997:Time Factors
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D012333:RNA, Messenger; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1; D019255:NADPH Oxidases; C086986:CYBA protein, human