title: Quantitative measurement of blood velocity in zebrafish with optical vector field tomography.
abstract: Microscopy techniques can readily visualize the finest details of embryo vasculature, but still lack to provide a complete three-dimensional representation of blood flow parameters. We present an in-vivo 3D imaging technique, able to reconstruct the blood cell velocity vector over a large volume of zebrafish embryos. This low cost and relatively simple technique is exploited to quantitatively assess blood velocity in the zebrafish tail at different stages of development.
authors: Politecnico di Milano, Dipartimento di Fisica, piazza Leonardo da Vinci 32, Milano, Italy.
authors: Luca
authors: L
authors: Fieramonti
authors: Efrem A
authors: EA
authors: Foglia
authors: Stefano
authors: S
authors: Malavasi
authors: Cosimo
authors: C
authors: D'Andrea
authors: Gianluca
authors: G
authors: Valentini
authors: Franco
authors: F
authors: Cotelli
authors: Andrea
authors: A
authors: Bassi
mesh_terms: D000818:Animals; D001775:Blood Circulation; D021621:Imaging, Three-Dimensional; D041622:Tomography, Optical / Q000379:methods*; D015027:Zebrafish / Q000502:physiology*
keywords: cardiovascular system; microscopy; optical tomography; velocimetry; zebrafish