title: Isolation and purification of arctigenin from Fructus Arctii by enzymatic hydrolysis combined with high-speed counter-current chromatography.
abstract: Enzymatic hydrolysis pretreatment combined with high-speed counter-current chromatography for the transformation and isolation of arctigenin from Fructus Arctii was successfully developed. In the first step, the extract solution of Fructus Arctii was enzymatic hydrolyzed by β-glucosidase. The optimal hydrolysis conditions were 40°C, pH 5.0, 24 h of hydrolysis time, and 1.25 mg/mL β-glucosidase concentration. Under these conditions, the content of arctigenin was transformed from 2.60 to 12.59 mg/g. In the second step, arctigenin in the hydrolysis products was separated and purified by high-speed counter-current chromatography with a two-phase solvent system composed of petroleum ether/ethyl acetate/methanol/water (10:25:15:20, v/v), and the fraction was analyzed by HPLC, ESI-MS, and (1)H NMR spectroscopy. Finally, 102 mg of arctigenin with a purity of 98.9% was obtained in a one-step separation from 200 mg of hydrolyzed sample.
authors: Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan, Shandong, China.
authors: Feng
authors: F
authors: Liu
authors: Xingjun
authors: X
authors: Xi
authors: Mei
authors: M
authors: Wang
authors: Li
authors: L
authors: Fan
authors: Yanling
authors: Y
authors: Geng
authors: Xiao
authors: X
authors: Wang
mesh_terms: D031170:Arctium / Q000737:chemistry*; D003377:Countercurrent Distribution; D005638:Fruit / Q000737:chemistry*; D005663:Furans / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D006868:Hydrolysis; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D005663:Furans; D017705:Lignans; D001617:beta-Glucosidase; C071942:arctigenin
keywords: Arctigenin; Enzymatic hydrolysis; Fructus Arctii; Glucosidase; High-speed counter-current chromatography