title: A highly sensitive dual-readout assay based on poly(A) and gold nanoparticles for palmatine hydrochloride.
abstract: This report presents a highly sensitive, poly(A)-stabilized gold nanoparticle-based assay with dual readouts (resonance light scattering and colorimetric) for detecting palmatine hydrochloride (PaH) in real samples. The detection mechanism is based on the fact that palmatine hydrochloride has strong affinity to poly(A), which can stabilize gold nanoparticles at high ionic strength, and cause the aggregation of poly(A)-stabilized AuNPs, resulting in the enhanced resonance light scattering (RLS). At the same time, the color change of poly(A)-stabilized AuNPs solution is from red to blue via purple. Thus a highly sensitive RLS assay for PaH has been developed with a linear range of 0.023-2.5 μg/mL. The limit of detection (LOD, 3σ) is 2.3 ng/mL. In this work, the reaction mechanism of this system was investigated by scanning electron microscope (SEM), dark-field light scattering images (DLSI), dynamiclight scattering (DLS) and circular dichroism (CD). This proposed method was also applied successfully for the determination of PaH in pharmaceutical preparations and urine samples with RSD⩽4.0%. The results are in good agreement with those from the official method.
authors: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address: tankj@swu.edu.cn.
authors: Kejun
authors: K
authors: Tan
authors: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors: Jiayu
authors: J
authors: Li
authors: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors: Huachun
authors: H
authors: Li
authors: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors: Yingying
authors: Y
authors: Wang
authors: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors: Rui
authors: R
authors: Yuan
mesh_terms: D000328:Adult; D001600:Berberine Alkaloids / Q000032:analysis* / Q000737:chemistry / Q000652:urine; D001681:Biological Assay / Q000379:methods*; D002214:Capsules; D002942:Circular Dichroism; D003124:Colorimetry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011061:Poly A / Q000737:chemistry*; D012542:Scattering, Radiation; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets
chemical_list: D001600:Berberine Alkaloids; D002214:Capsules; D013607:Tablets; D011061:Poly A; D006046:Gold; C005413:palmatine
keywords: Gold nanoparticles; Palmatine hydrochloride; Poly(A); Resonance light scattering