title: Identification and molecular characterization of a single-stranded circular DNA virus with similarities to Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1.
abstract: A putative circular single-stranded DNA (ssDNA) virus was recovered from Hypericum japonicum collected in Vietnam. The viral isolate was tentatively named Hypericum japonicum-associated circular DNA virus (HJasCV). HJasCV shares 58.7-65.4% nucleotide sequence identity with Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1) and SsHADV-1-like viruses. Like this group of viruses, the genome of HJasCV (2 200 nt) has two large ORFs, one in the virion-sense and the other in the complementary-sense DNA. The proteins encoded in the virion-sense and complementary-sense ORFs share 39-46 % and 45-67 % amino acid sequence identity with the putative capsid and replication-associated proteins (Reps), respectively, of SsHADV-1 and SsHADV-1-like viruses. The putative Rep of HJasCV contains all of the motifs related to rolling-circle replication. Its 111-bp intergenic region (IR) contains a hairpin structure with a geminivirus-like nonanucleotide sequence, TAATGTTAT, at the apex of the loop. Phylogenetic analysis revealed that HJasCV forms a monophyletic clade with SsHADV-1 and SsHADV-1-like viruses.
authors: Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
authors: Zhenguo
authors: Z
authors: Du
authors: Yafei
authors: Y
authors: Tang
authors: Songbai
authors: S
authors: Zhang
authors: Xiaoman
authors: X
authors: She
authors: Guobing
authors: G
authors: Lan
authors: Arvind
authors: A
authors: Varsani
authors: Zifu
authors: Z
authors: He
mesh_terms: D020816:Amino Acid Motifs; D016000:Cluster Analysis; D004267:DNA Viruses / Q000235:genetics* / Q000302:isolation & purification*; D004270:DNA, Circular / Q000235:genetics*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D020902:Hypericum / Q000821:virology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014744:Vietnam
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral