title: A genetic variant in p63 (rs17506395) is associated with breast cancer susceptibility and prognosis.
abstract: BACKGROUND
  p63, homologous to p53, has been investigated to be involved in various aspects of tumorigenesis and cancer progression. Recently, we have identified a functional single nucleotide polymorphism (SNP) rs17506395 (T>G) in p63 which was associated with female reproduction and ovarian cancer development. The present study aimed to evaluate the association of rs17506395 genotypes with breast cancer susceptibility, clinicopathological characteristics and prognosis.
  
  
  METHODS
  TaqMan assay was used to genotype the rs17506395 polymorphism. The expression of p63 mRNA was determined by quantitative reverse-transcription PCR (qRT-PCR). Unconditional logistic regression and univariate Cox hazard regression analyses were performed to evaluate the association of rs17506395 with breast cancer susceptibility and prognosis respectively.
  
  
  RESULTS
  First, we found that breast tumors with TT genotype exhibited higher level of p63 mRNA compared with other genotypes in breast cancer tissues, indicating that rs17506395 may be a functional single nucleotide polymorphism in breast cancer. Further investigation revealed that the presence of TT genotype was statistically correlated with increased risk for breast cancer, compared with genotypes containing the G allele (GG and GT). Moreover, a significant association between rs17506395 polymorphism and age at diagnosis and status of hormone receptor was observed. Consistently, prognostic analysis showed that patients carrying TT genotype represent unfavorable survival, suggesting that TT genotype may be a biomarker for poor prognosis in breast cancer.
  
  
  CONCLUSIONS
  Our data suggest that rs17506395 polymorphism can function as a risk factor and prognostic indicator for breast cancer, whereas precise mechanism underlying the function of this polymorphism needs further investigation.
authors: Department of Breast Surgery, Qilu Hospital, Shandong University, Shandong, China.
authors: Ning
authors: N
authors: Zhang
authors: Department of Breast Surgery, Qilu Hospital, Shandong University, Shandong, China.
authors: Qiang
authors: Q
authors: Huo
authors: Department of Breast Surgery, Qilu Hospital, Shandong University, Shandong, China.
authors: Xiaolong
authors: X
authors: Wang
authors: Department of Breast Surgery, Qilu Hospital, Shandong University, Shandong, China.
authors: Xi
authors: X
authors: Chen
authors: Department of Breast Surgery, Qilu Hospital, Shandong University, Shandong, China.
authors: Li
authors: L
authors: Long
authors: Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Shandong, China.
authors: Xiao
authors: X
authors: Guan
authors: Department of Breast Surgery, Qilu Hospital, Shandong University, Shandong, China.
authors: Liyu
authors: L
authors: Jiang
authors: Department of Breast Surgery, Qilu Hospital, Shandong University, Shandong, China.
authors: Tingting
authors: T
authors: Ma
authors: Department of Radiation Oncology, Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey, New Brunswick, NJ, USA; Department of Pediatrics, Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey, New Brunswick, NJ, USA.
authors: Wenwei
authors: W
authors: Hu
authors: Department of Breast Surgery, Qilu Hospital, Shandong University, Shandong, China; Key Laboratory of Experimental Teratology, Ministry of Education and Institute of Molecular Medicine and Genetics, Shandong University School of Medicine, Jinan, China. Electronic address: qifengy@gmail.com.
authors: Qifeng
authors: Q
authors: Yang
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D001943:Breast Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C115051:TP63 protein, human; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins
keywords: Breast cancer; ER; HER2; HWE; Hardy–Weinberg equilibrium; IHC; PCR; PR; Prognosis; SNP; SNPs; Single nucleotide polymorphism; Susceptibility; estrogen receptor; human epidermal growth factor receptor 2; immunohistochemistry; p63; polymerase chain reaction; progesterone receptor; single nucleotide polymorphisms