title: Differential role of the basolateral amygdala 5-HT3 and 5-HT4 serotonin receptors upon ACPA-induced anxiolytic-like behaviors and emotional memory deficit in mice.
abstract: OBJECTIVE
  The critical role of cannabinoidergic and serotonergic systems of the amygdala in modulation of anxiety-like behaviors and emotional memory has already been demonstrated. The present study aimed to investigate the possible role of the basolateral amygdala (BLA) 5-HT3 and 5-HT4 serotonergic systems upon ACPA (CB1 cannabinoid receptor agonist)-induced anxiolytic-like behaviors and emotional memory impairment using the elevated plus-maze (EPM) test-retest paradigm in male mice.
  
  
  METHODS
  bilateral guide-cannulae were implanted to allow intra-BLA microinjection of serotonergic agents.
  
  
  RESULTS
  the intraperitoneal injection of ACPA could induce anxiolytic-like behaviors and reduce the emotional memory formation. Intra-BLA injection of M-Chlorophenylbiguanide (M-Chl, a 5-HT3 serotonin receptor agonist) neither altered the anxiety-like behaviors nor the emotional memory formation by itself, while the higher dose of Y-25130 (a 5-HT3 serotonin receptor antagonist) reduced the emotional memory formation and locomotor activity but not the anxiety-like behaviors. Furthermore, injection of a higher dose of RS67333 and RS23597 (as 5-HT4 serotonin receptor agonist and antagonist, respectively) did not alter the anxiety-like behaviors, while reduced the emotional memory formation. In addition, the intra-BLA injection of M-Chl but not Y-25130 and RS67333 restored the ACPA-induced anxiolytic-like behaviors and emotional memory deficit, while a higher dose of RS67333 decreased the locomotor activity. Moreover, the intra-BLA microinjection of RS23597 could restore the ACPA-induced anxiolytic-like behaviors but not the emotional memory deficit.
  
  
  CONCLUSIONS
  based on our findings, ACPA seems to induce its anxiolytic-like behaviors and emotional memory formation deficits via activation and deactivation of the BLA 5-HT4 and 5-HT3 serotonin receptors.
authors: Institute for Cognitive Science Studies (ICSS), Tehran, Iran.
authors: Hamid-Reza
authors: HR
authors: Chegini
authors: Institute for Cognitive Science Studies (ICSS), Tehran, Iran; Department of Biology, Faculty of Basic Sciences, Garmsar Branch, Islamic Azad University, Garmsar, P.O. Box 13145-784, Iran. Electronic address: Nasehi@iricss.org.
authors: Mohammad
authors: M
authors: Nasehi
authors: Institute for Cognitive Science Studies (ICSS), Tehran, Iran; Department of Neuroscience, School of Advanced Technologies in medicine and Department of Pharmacology School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Iranian National Center for Addiction Studies, Tehran University of Medical Sciences, Tehran, Iran; School of Cognitive Sciences, Institute for Research in Fundamental Sciences (IPM), Tehran, Iran. Electronic address: zarinmr@ams.ac.ir.
authors: Mohammad-Reza
authors: MR
authors: Zarrindast
mesh_terms: D000679:Amygdala / Q000187:drug effects / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D001008:Anxiety Disorders / Q000139:chemically induced*; D001095:Arachidonic Acids / Q000633:toxicity*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D008569:Memory Disorders / Q000139:chemically induced*; D051379:Mice; D008845:Microinjections; D043884:Receptor, Cannabinoid, CB1 / Q000819:agonists; D044406:Receptors, Serotonin, 5-HT3 / Q000378:metabolism*; D044422:Receptors, Serotonin, 5-HT4 / Q000378:metabolism*; D018490:Serotonin Agents / Q000494:pharmacology
chemical_list: D001095:Arachidonic Acids; D043884:Receptor, Cannabinoid, CB1; D044406:Receptors, Serotonin, 5-HT3; D018490:Serotonin Agents; C119324:arachidonylcyclopropylamide; D044422:Receptors, Serotonin, 5-HT4
keywords: Anxiety; Elevated plus-maze; Emotional memory; Mouse; Serotonergic agent