title: Identification of quantitative trait locus (QTL) linked to dorsal fin length from preliminary linkage map of molly fish, Poecilia sp.
abstract: A preliminary linkage map was constructed by applying backcross and testcross strategy using microsatellite (SSR) markers developed for Xiphophorus and Poecilia reticulata in ornamental fish, molly Poecilia sp. The linkage map having 18 SSR loci consisted of four linkage groups that spanned a map size of 516.1cM. Association between genotypes and phenotypes was tested in a random fashion and QTL for dorsal fin length was found to be linked to locus Msb069 on linkage group 2. Coincidentally, locus Msb069 was also reported as putative homologue primer pairs containing SSRs repeat motif which encoded hSMP-1, a sex determining locus. Dorsal fin length particularly in males of Poecilia latipinna is an important feature during courtship display. Therefore, we speculate that both dorsal fin length and putative hSMP-1 gene formed a close proximity to male sexual characteristics.
authors: Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang Selangor, Malaysia. Electronic address: bunpoh@yahoo.com.
authors: Bun Poh
authors: BP
authors: Keong
authors: Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang Selangor, Malaysia.
authors: Siti Shapor
authors: SS
authors: Siraj
authors: Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang Selangor, Malaysia.
authors: Siti Khalijah
authors: SK
authors: Daud
authors: Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang Selangor, Malaysia.
authors: Jothi Malar
authors: JM
authors: Panandam
authors: Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang Selangor, Malaysia.
authors: Arina Nadia Abdul
authors: AN
authors: Rahman
mesh_terms: D058500:Animal Fins / Q000033:anatomy & histology*; D000818:Animals; D002874:Chromosome Mapping / Q000379:methods / Q000662:veterinary; D005260:Female; D008040:Genetic Linkage; D008297:Male; D009929:Organ Size / Q000235:genetics; D011036:Poecilia / Q000033:anatomy & histology* / Q000235:genetics*; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci
keywords: BC; Backcross; Base pairs; Centimorgan; Coefficient of determination; DFL; Degree celsius; Dorsal fin length; F(1); F(2); First filial generation; LG; LOD; Linkage group; Linkage map; Logarithm of odds; Male courtship display; Microlitre; Microsatellites; Minute; Number; P; Poecilia latipinna; Probability; QTL; Quantitative Trait Loci; Quantitative trait loci; R(2); SSRs; Second filial generation; Species; USA; United States of America; bp; cM; min; n; sp.; °C; μl