title: Crystallization and preliminary X-ray diffraction studies of the (R)-selective amine transaminase from Aspergillus fumigatus.
abstract: The (R)-selective amine transaminase from Aspergillus fumigatus was expressed in Escherichia coli and purified to homogeneity. Bright yellow crystals appeared while storing the concentrated solution in the refrigerator and belonged to space group C222(1). X-ray diffraction data were collected to 1.27 Å resolution, as well as an anomalous data set to 1.84 Å resolution that was suitable for S-SAD phasing.
authors: Institut für Biochemie, Universität Greifswald, Felix-Hausdorff-Strasse 4, D-17489 Greifswald, Germany.
authors: Maren
authors: M
authors: Thomsen
authors: Lilly
authors: L
authors: Skalden
authors: Gottfried J
authors: GJ
authors: Palm
authors: Matthias
authors: M
authors: Höhne
authors: Uwe T
authors: UT
authors: Bornscheuer
authors: Winfried
authors: W
authors: Hinrichs
mesh_terms: D000588:Amines / Q000737:chemistry* / Q000378:metabolism; D001232:Aspergillus fumigatus / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013455:Sulfur / Q000737:chemistry; D000637:Transaminases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D000588:Amines; D005656:Fungal Proteins; D011994:Recombinant Proteins; D013455:Sulfur; D000637:Transaminases
keywords: (R)-selective amine transaminase; S-SAD