title: Analysis of the heat-shock protein pattern during somatic embryogenesis of carrot.
abstract: The pattern of proteins synthesized by carrot cells after heat-shock was analyzed by SDS-polyacrylamide gel electrophoresis. A study was made of the time of appearance and disappearance of the heat-shock bands and a comparison was made of the heat-shock proteins present in cell extracts obtained from cell suspensions, callus, protoplasts and the different stages of somatic embryogenesis.From this comparison a number of differences and hence of stage-specific markers could be seen. This type of analysis was also carried out in the presence of α-amanitin in order to get information on the type of control of heat-shock protein synthesis. It turned out that in all stages after the initial globular stage, α-amanitin does not alter the heat-shock protein pattern suggesting pre-existence of the relative mRNA's.
authors: Istituto di Mutagenesi e Differenziamento, CNR, Via Svezia 10, I-56100, Pisa, Italy.
authors: L
authors: L
authors: Pitto
authors: F L
authors: FL
authors: Schiavo
authors: G
authors: G
authors: Giuliano
authors: M
authors: M
authors: Terzi