title: Immunoadjuvant efficacy of N-carboxymethyl chitosan for vaccination via dendritic cell activation.
abstract: To induce humoral- and cell-mediated immune responses in protein-based vaccinations, immunoadjuvants are needed. In this study, we investigated the immunoadjuvant effect of N-carboxymethyl chitosan (N-CMCh) on the vaccination system through dendritic cell activation. The results showed that N-CMCh nanoparticle enhanced the secretion of cytokines (interleukin [IL]-6, IL-12p70, and tumor necrosis factor-α) and antigen uptake in bone marrow-derived dendritic cells. This activated antigen-specific Th1 cell responses, including IL-2 and interferon-gamma production and proliferation. In addition, N-CMCh delayed the dissemination of ovalbumin (OVA) from an injection site in female C57BL/6 mice. As a result, in the subcutaneous vaccination, OVA-N-CMCh nanoparticles enhanced both humoral and cell-mediated vaccine responses.
authors: 1 Department of Biology, Sunchon National University , Suncheon, Korea.
authors: Jong-Jin
authors: JJ
authors: Kim
authors: Joung-Pyo
authors: JP
authors: Nam
authors: Jae-Woon
authors: JW
authors: Nah
authors: Mi-Kyeong
authors: MK
authors: Jang
authors: Sung-Tae
authors: ST
authors: Yee
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D002478:Cells, Cultured; D048271:Chitosan / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D018664:Interleukin-12 / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D014611:Vaccination
chemical_list: D000276:Adjuvants, Immunologic; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; C514968:carboxymethyl-chitosan; D018664:Interleukin-12; D010047:Ovalbumin; D048271:Chitosan