title: A novel autoantibody against moesin in the serum of patients with MPO-ANCA-associated vasculitis.
abstract: BACKGROUND
  Antineutrophil cytoplasmic autoantibody (ANCA) directed against myeloperoxidase (MPO), a diagnostic criterion in MPO-ANCA-associated vasculitis (MPO-AAV), does not always correlate with disease activity. Here, we detected autoantibodies against moesin, which was located on the surface of stimulated endothelial cells, in the serum of patients.
  
  
  METHODS
  The anti-moesin autoantibody titer was evaluated by ELISA. Seventeen kinds of cytokines/chemokines were measured by a Bio-Plex system.
  
  
  RESULTS
  Serum creatinine in the anti-moesin autoantibody-positive group was higher than that in the negative group. Additionally, interferon (IFN)-γ, macrophage chemotactic peptide-1 (MCP-1), interleukin (IL)-2, IL-7, IL-12p70, IL-13, granulocyte/macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor were significantly higher in the positive group. Furthermore, IL-7 and IL-12p70 levels correlated with the anti-moesin autoantibody titer. Based on these findings and the binding of anti-moesin IgG to neutrophils and monocytes, we detected the secretion of cytokines/chemokines such as IFN-γ, MCP-1 and GM-CSF from these cells.
  
  
  CONCLUSIONS
  The anti-moesin autoantibody existed in the serum of patients with MPO-AAV and was associated with the production of inflammatory cytokines/chemokines targeting neutrophils with a cytoplasmic profile, which suggests that the anti-moesin autoantibody has the possibility to be a novel autoantibody developing vasculitis via neutrophil and endothelial cell activation.
authors: Inflammation Program, Chiba University, Graduate School of Medicine, Chiba City, Japan Graduate School of Life and Environmental Science, Tsukuba University, Tsukuba, Ibaragi, Japan Laboratory of Biological Science, Graduate School of Frontier Biosciences, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.
authors: Koya
authors: K
authors: Suzuki
authors: Inflammation Program, Chiba University, Graduate School of Medicine, Chiba City, Japan.
authors: Tomokazu
authors: T
authors: Nagao
authors: Department of Internal Medicine, Tokyo Women's Medical University, Tokyo, Japan.
authors: Mitsuyo
authors: M
authors: Itabashi
authors: Department of Internal Medicine, Jichi University, School of Medicine, Shimotsuke, Tochigi, Japan.
authors: Yoshitomo
authors: Y
authors: Hamano
authors: Inflammation Program, Chiba University, Graduate School of Medicine, Chiba City, Japan.
authors: Ryuichi
authors: R
authors: Sugamata
authors: Laboratory of Biological Science, Graduate School of Frontier Biosciences, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.
authors: Yuji
authors: Y
authors: Yamazaki
authors: Department of Internal Medicine, Jichi University, School of Medicine, Shimotsuke, Tochigi, Japan.
authors: Wako
authors: W
authors: Yumura
authors: Laboratory of Biological Science, Graduate School of Frontier Biosciences, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.
authors: Sachiko
authors: S
authors: Tsukita
authors: Graduate School of Life and Environmental Science, Tsukuba University, Tsukuba, Ibaragi, Japan.
authors: Pi-Chao
authors: PC
authors: Wang
authors: Department of Immunology, Chiba University, Graduate School of Medicine, Chiba City, Japan.
authors: Toshinori
authors: T
authors: Nakayama
authors: Inflammation Program, Chiba University, Graduate School of Medicine, Chiba City, Japan Asia International Institute of Infectious Disease Control, Department of Health Protection, Graduate School of Medicine, Teikyo University, Tokyo, Japan.
authors: Kazuo
authors: K
authors: Suzuki
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D056648:Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D018925:Chemokines / Q000378:metabolism; D004730:Endothelium, Vascular / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood; D006801:Humans; D007378:Interleukins / Q000276:immunology; D019402:Macrophage Inflammatory Proteins / Q000097:blood; D008297:Male; D008840:Microfilament Proteins / Q000276:immunology*; D008875:Middle Aged; D009195:Peroxidase / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D018925:Chemokines; D007378:Interleukins; D019402:Macrophage Inflammatory Proteins; D008840:Microfilament Proteins; D016179:Granulocyte Colony-Stimulating Factor; C071058:moesin; D009195:Peroxidase
keywords: autoantibodies; glomerulonephritis; moesin; myeloperoxidase; neutrophil