title: Citrange fruit extracts alleviate obesity-associated metabolic disorder in high-fat diet-induced obese C57BL/6 mouse.
abstract: Obesity is becoming one of the global epidemics of the 21st century. In this study, the effects of citrange (Citrus sinensis × Poncirus trifoliata) fruit extracts in high-fat (HF) diet-induced obesity mice were studied. Female C57BL/6 mice were fed respectively a chow diet (control), an HF diet, HF diet supplemented with 1% w/w citrange peel extract (CPE) or 1% w/w citrange flesh and seed extract (CFSE) for 8 weeks. Our results showed that both CPE and CFSE regulated the glucose metabolic disorders of obese mice. In CPE and CFSE-treated groups, the body weight gain, blood glucose, serum total cholesterol (TC) and low density lipoprotein cholesterol (LDL-c) levels were significantly (p<0.05) reduced relative to those in the HF group. To explore the mechanisms of action of CPE and CFSE on the metabolism of glucose and lipid, related genes' expressions in liver were assayed. In liver tissue, the expression level of peroxisome proliferator-activated receptor γ (PPARγ) and its target genes were down-regulated by CPE and CFSE supplementation as revealed by qPCR tests. In addition, both CPE and CFSE decreased the expression level of liver X receptor (LXR) α and β, which are involved in lipid and glucose metabolism. Taken together, these results suggest that CPE and CFSE administration could ameliorate obesity and related metabolic disorders in HF diet-induced obesity mice probably through the inhibition of PPARγ and LXRs gene expressions.
authors: College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China. chuang.shutcm@gmail.com.
authors: Yan
authors: Y
authors: Lu
authors: Wanpeng
authors: W
authors: Xi
authors: Xiaobo
authors: X
authors: Ding
authors: Shengjie
authors: S
authors: Fan
authors: Yu
authors: Y
authors: Zhang
authors: Dong
authors: D
authors: Jiang
authors: Yiming
authors: Y
authors: Li
authors: Cheng
authors: C
authors: Huang
authors: Zhiqin
authors: Z
authors: Zhou
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D059305:Diet, High-Fat; D005260:Female; D005419:Flavonoids / Q000032:analysis; D005638:Fruit / Q000737:chemistry / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D005951:Glucose Tolerance Test; D008099:Liver / Q000378:metabolism / Q000473:pathology; D000071518:Liver X Receptors; D008659:Metabolic Diseases / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000150:complications; D057093:Orphan Nuclear Receptors / Q000235:genetics / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000378:metabolism; D012639:Seeds / Q000737:chemistry / Q000378:metabolism; D015430:Weight Gain / Q000187:drug effects
chemical_list: D005419:Flavonoids; D000071518:Liver X Receptors; C000605214:Nr1h3 protein, mouse; D057093:Orphan Nuclear Receptors; D047495:PPAR gamma; D010936:Plant Extracts; D012333:RNA, Messenger; D002784:Cholesterol