title: Interleukin-8 responses of multi-layer gingival epithelia to subgingival biofilms: role of the "red complex" species.
abstract: Periodontitis is an infectious inflammatory disease that results in the destruction of the tooth-supporting (periodontal) tissues. The Gram-negative anaerobic species Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola, (also known as the "red complex" species) are highly associated with subgingival biofilms at periodontitis-affected sites. A major chemokine produced by the gingival epithelium in response to biofilm challenge, is interleukin (IL)-8. The aim of this in vitro study was to investigate the relative effect of the "red complex" species as constituents of subgingival biofilms, on the regulation of IL-8 by gingival epithelia. Multi-layered organotypic human gingival epithelial cultures were challenged with a 10-species in vitro subgingival biofilm model, or its 7-species variant, excluding the "red complex". IL-8 gene expression and secretion analyses were performed by qPCR and ELISA, respectively. After 3 h, both biofilms up-regulated IL-8 gene expression, but the presence of the "red complex" resulted in 3-fold greater response. IL-8 secretion was also up-regulated by both biofilms, with no differences between them. After 24 h, the 10-species biofilm reduced IL-8 secretion to 50% of the control, but this was not affected when the "red complex" was absent. In conclusion, as part of biofilms, "red complex" species differentially regulate IL-8 in gingival epithelia, potentially affecting the chemotactic responses of the tissue.
authors: Oral Microbiology and Immunology, Institute of Oral Biology, Center of Dental Medicine, University of Zürich, Zürich, Switzerland.
authors: Georgios N
authors: GN
authors: Belibasakis
authors: Thomas
authors: T
authors: Thurnheer
authors: Nagihan
authors: N
authors: Bostanci
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology*; D005881:Gingiva / Q000166:cytology* / Q000382:microbiology*; D006090:Gram-Negative Bacteria / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D013997:Time Factors
chemical_list: D016209:Interleukin-8