title: Decreased expression of myosin light chain MYL9 in stroma predicts malignant progression and poor biochemical recurrence-free survival in prostate cancer.
abstract: The aim of this study was to investigate the associations of myosin light chain (MYL9) downregulation with tumor progression and prognosis in patients with prostate cancer (PCa). MYL9 protein expression in human PCa and non-cancerous prostate tissues was detected by Western blot and immunohistochemistry analyses, which was validated by microarray-based Taylor data at mRNA level. Then, the associations of MYL9 expression with clinicopathological features and clinical outcome of PCa patients were statistically analyzed. Both Western blot and immunohistochemistry analyses found that MYL9 expression was significantly decreased (both P < 0.001) in PCa tissues compared with those in non-cancerous prostate tissues. In addition, MYL9 was mainly expressed in the cytoplasm of stromal cells of prostate tissues, and the decreased expression of MYL9 in PCa tissues was significantly correlated with the older age of patients (P = 0.011), the higher Gleason score (P < 0.001), the advanced pathological stage (P = 0.002), the presence of metastasis (P < 0.001) and PSA failure (P = 0.001). Furthermore, both univariate and multivariate analyses showed that the downregulation of MYL9 was an independent predictor of shorter overall survival (P = 0.026 and P = 0.009, respectively) and biochemical recurrence-free survival (P = 0.001 and P = 0.002, respectively). Our data strongly confirmed for the first time that the decreased expression of MYL9 may play an important role in tumor progression of PCa. More importantly, the downregulation of MYL9 may efficiently predict both overall and biochemical recurrence-free survivals in PCa patients.
authors: Guangdong Provincial Institute of Nephrology, Southern Medical University, Guangzhou, 510515, China.
authors: Ya-Qiang
authors: YQ
authors: Huang
authors: Zhao-Dong
authors: ZD
authors: Han
authors: Yu-Xiang
authors: YX
authors: Liang
authors: Zhuo-Yuan
authors: ZY
authors: Lin
authors: Xiao-Hui
authors: XH
authors: Ling
authors: Xin
authors: X
authors: Fu
authors: Chao
authors: C
authors: Cai
authors: Xue-Cheng
authors: XC
authors: Bi
authors: Qi-Shan
authors: QS
authors: Dai
authors: Jia-Hong
authors: JH
authors: Chen
authors: Hui-Chan
authors: HC
authors: He
authors: Yan-Ru
authors: YR
authors: Chen
authors: Fu-Neng
authors: FN
authors: Jiang
authors: Wei-de
authors: WD
authors: Zhong
mesh_terms: D000368:Aged; D018450:Disease Progression; D018572:Disease-Free Survival; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009218:Myosins / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D011467:Prostate / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; C113469:myosin IXB; D009218:Myosins