title: Microbial fingerprinting detects intestinal microbiota dysbiosis in Zebrafish models with chemically-induced enterocolitis.
abstract: BACKGROUND
  Inflammatory bowel disease (IBD) involves a breakdown in interactions between the host immune response and the resident commensal microbiota. Recent studies have suggested gut physiology and pathology relevant to human IBD can be rapidly modeled in zebrafish larvae. The aim of this study was to investigate the dysbiosis of intestinal microbiota in zebrafish models with IBD-like enterocolitis using culture-independent techniques.
  
  
  RESULTS
  IBD-like enterocolitis was induced by exposing larval zebrafish to trinitrobenzenesulfonic acid (TNBS). Pathology was assessed by histology and immunofluorescence. Changes in intestinal microbiota were evaluated by denaturing gradient gel electrophoresis (DGGE) and the predominant bacterial composition was determined with DNA sequencing and BLAST and confirmed by real-time polymerase chain reaction. Larval zebrafish exposed to TNBS displayed intestinal-fold architecture disruption and inflammation reminiscent of human IBD. In this study, we defined a reduced biodiversity of gut bacterial community in TNBS-induced colitis. The intestinal microbiota dysbiosis in zebrafish larvae with IBD-like colitis was characterized by an increased proportion of Proteobacteria (especially Burkholderia) and a decreased of Firmicutes(Lactobacillus group), which were significantly correlated with enterocolitis severity(Pearson correlation p < 0.01).
  
  
  CONCLUSIONS
  This is the first description of intestinal microbiota dysbiosis in zebrafish IBD-like models, and these changes correlate with TNBS-induced enterocolitis. Prevention or reversal of this dysbiosis may be a viable option for reducing the incidence and severity of human IBD.
authors: Qi
authors: Q
authors: He
authors: Lin
authors: L
authors: Wang
authors: Fan
authors: F
authors: Wang
authors: Chenyang
authors: C
authors: Wang
authors: Chun
authors: C
authors: Tang
authors: Research Institute of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, No,305 East Zhongshan Road, Nanjing 210002, China. qiurongli12@gmail.com.
authors: Qiurong
authors: Q
authors: Li
authors: Jieshou
authors: J
authors: Li
authors: Qingshun
authors: Q
authors: Zhao
mesh_terms: D000818:Animals; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D004195:Disease Models, Animal; D064806:Dysbiosis; D004760:Enterocolitis / Q000139:chemically induced* / Q000382:microbiology* / Q000473:pathology; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014302:Trinitrobenzenesulfonic Acid / Q000008:administration & dosage / Q000633:toxicity; D015027:Zebrafish
chemical_list: D004269:DNA, Bacterial; D014302:Trinitrobenzenesulfonic Acid