title: [Immune regulatory effect of citalopram on microglial cells].
abstract: OBJECTIVE
  To investigate the effects of antidepressant citalopram on the gene expressions of tumor necrosis factor-alpha (TNF-α) and interleukin 1 beta (IL-1β), and to discuss the impacts of citalopram on p38 and c-jun N-terminal kinase (JNK) of mitogen-activated protein kinase (MAPK) family in microglial cells.
  
  
  METHODS
  BV2 cells were induced by lipopolysaccharide (LPS) to produce TNF-α and IL-1β. After pretreatment with citalopram (20 μmol/L) for 4 h, the mRNA levels of TNF-α and IL-1β were measured by quantitative real-time PCR (qRT-PCR); after pretreatment for 24 h, the protein levels of TNF-α and IL-1β were analyzed by ELISA; the effects of citalopram on the phosphorylation of p38MAPK and JNK were observed after pretreatment for 30 min.
  
  
  RESULTS
  Citalopram significantly inhibited the mRNA and protein expressions of TNF-α and IL-1β, and the phosphorylation of p38MAPK and JNK.
  
  
  CONCLUSIONS
  Citalopram may play the anti-inflammatory role by inhibiting MAPK pathway in microglial cells.
authors: Department of Neurology, Nanlou Clinical Division, PLA General Hospital, Beijing 100853, China.
authors: Shanshan
authors: S
authors: Wang
authors: Haiying
authors: H
authors: Chen
authors: Hengge
authors: H
authors: Xie
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology*; D002460:Cell Line; D015283:Citalopram / Q000494:pharmacology*; D007155:Immunologic Factors / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000235:genetics; D020935:MAP Kinase Signaling System / Q000187:drug effects; D017628:Microglia / Q000166:cytology / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D000928:Antidepressive Agents; D007155:Immunologic Factors; D053583:Interleukin-1beta; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D015283:Citalopram