title: Isolation and characterization of a Δ1-pyrroline-5-carboxylate synthetase (NtP5CS) from Nitraria tangutorum Bobr. and functional comparison with its Arabidopsis homologue.
abstract: Several functional and regulatory proteins play important roles in controlling plant stress tolerance. Proline (Pro) is one of the most accumulated osmolytes correlated with tolerance to stresses. Δ(1)-Pyrroline-5-carboxylate synthetase (P5CS) is a rate-limiting enzyme in Pro biosynthesis. In the present study, we isolated the cDNA for a P5CS gene (NtP5CS) from the halophyte Nitraria tangutorum. Phylogenetic analysis and subcellular localization analysis of NtP5CS-GFP protein in onion cells showed that NtP5CS was a new P5CS gene and was involved in Pro synthesis in N. tangutorum. Expression of the NtP5CS gene was induced by salt stress, dehydration, and high and low temperatures. Escherichia coli overexpressing AtP5CS or NtP5CS exhibited better growth in all treatments, including high salinity, high alkalinity, dehydration, osmotic, heat and cold stresses. Additionally, NtP5CS recombinant E. coli cells grew better than did AtP5CS recombinant cells in response to abiotic stresses. Our data demonstrate that the P5CS from a halophytic species functions more efficiently than its homologue from a glycophytic species in improving the stress tolerance of E. coli.
authors: Key Laboratory of Herbage & Endemic Crop Biotechnology, College of Life Sciences, Inner Mongolia University, Hohhot, 010020, People's Republic of China.
authors: Linlin
authors: L
authors: Zheng
authors: Zhenhua
authors: Z
authors: Dang
authors: Haoyu
authors: H
authors: Li
authors: Huirong
authors: H
authors: Zhang
authors: Shubiao
authors: S
authors: Wu
authors: Yingchun
authors: Y
authors: Wang
mesh_terms: D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000235:genetics; D003001:Cloning, Molecular; D019669:Embryophyta / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development; D004926:Escherichia coli; D050698:Glutamate-5-Semialdehyde Dehydrogenase / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000096:biosynthesis* / Q000235:genetics; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis* / Q000235:genetics; D010802:Phylogeny; D019441:Plant Epidermis / Q000166:cytology / Q000378:metabolism; D021381:Protein Transport; D055049:Salt Tolerance; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; D009097:Multienzyme Complexes; C583835:delta(1)-pyrroline-5-carboxylate synthetase, Arabidopsis; D050698:Glutamate-5-Semialdehyde Dehydrogenase; D017853:Phosphotransferases (Alcohol Group Acceptor)