title: Parvilucifera rostrata sp. nov. (Perkinsozoa), a novel parasitoid that infects planktonic dinoflagellates.
abstract: The diversity and ecological roles of protists in marine plankton are still poorly known. In 2011, we made a substantial effort to isolate parasites into cultures during the course of blooms of the toxic microalga Alexandrium minutum (Dinophyceae) in two estuaries (the Penzé and the Rance, Brittany coast, north-west of France). In total, 99 parasitic strains were obtained. Screening of ribosomal internal transcribed spacer regions (including ITS1, 5.8S and ITS2) revealed the existence of two ribotypes. Small subunit and partial large subunit rRNA genes revealed that these two ribotypes belong to different species of the genus Parvilucifera. The first ribotype was tentatively affiliated to the species Parvilucifera infectans, whilst the second represents a new species, Parvilucifera rostrata sp. nov. The new species has several distinct morphological features in the general organization of its zoospore and in the shape and size of processes covering the sporangium. Both Parvilucifera species are generalist parasitoids with similar generation times, and this study thus raises the question of how two parasitoids exploiting similar ecological resources and infection strategies can coexist in the same ecosystem. Taxonomic relationships between Parvilucifera spp. and other closely related marine parasitoids, such as syndinians, are discussed.
authors: CNRS, UMR 7144, Station Biologique, Place Georges Teissier, CS90074, 29688 Roscoff Cedex, France; Université Pierre et Marie Curie (Paris VI), Station Biologique, Place Georges Teissier, CS90074, 29688 Roscoff Cedex, France.
authors: Frédéric
authors: F
authors: Lepelletier
authors: Zoological Institute RAS, St. Petersburg, Russia; St. Petersburg State University, St. Petersburg, Russia.
authors: Sergey A
authors: SA
authors: Karpov
authors: CNRS, FR 2424, Plate-forme Merimage, Station Biologique de Roscoff, Place Georges Teissier, CS90074, 29688 Roscoff Cedex, France; Université Pierre et Marie Curie (Paris VI), Station Biologique, Place Georges Teissier, CS90074, 29688 Roscoff Cedex, France.
authors: Sophie
authors: S
authors: Le Panse
authors: CNRS, UMR 7144, Station Biologique, Place Georges Teissier, CS90074, 29688 Roscoff Cedex, France; Université Pierre et Marie Curie (Paris VI), Station Biologique, Place Georges Teissier, CS90074, 29688 Roscoff Cedex, France.
authors: Estelle
authors: E
authors: Bigeard
authors: Laboratory of Aquatic Pathobiology, Department of Veterinary Disease Biology, University of Copenhagen, Frederiksberg, Denmark.
authors: Alf
authors: A
authors: Skovgaard
authors: CNRS, UMR 7144, Station Biologique, Place Georges Teissier, CS90074, 29688 Roscoff Cedex, France; Université Pierre et Marie Curie (Paris VI), Station Biologique, Place Georges Teissier, CS90074, 29688 Roscoff Cedex, France.
authors: Christian
authors: C
authors: Jeanthon
authors: CNRS, UMR 7144, Station Biologique, Place Georges Teissier, CS90074, 29688 Roscoff Cedex, France; Université Pierre et Marie Curie (Paris VI), Station Biologique, Place Georges Teissier, CS90074, 29688 Roscoff Cedex, France. Electronic address: lguillou@sb-roscoff.fr.
authors: Laure
authors: L
authors: Guillou
mesh_terms: D056893:Alveolata / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*; D059001:Aquatic Organisms / Q000469:parasitology; D016000:Cluster Analysis; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D004141:Dinoflagellida / Q000469:parasitology*; D017753:Ecosystem; D005602:France; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D016053:RNA, Protozoan / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D016053:RNA, Protozoan; D012335:RNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D012340:RNA, Ribosomal, 5.8S; C065096:RNA, ribosomal, 26S
keywords: Alexandrium minutum; Parvilucifera; dinoflagellate; parasitoid; toxic blooms.