title: Characterization of Lin-ve CD34 and CD117 cell population reveals an increased expression in bone marrow derived stem cells.
abstract: The purpose of the study was to evaluate the expression of CD45, CD34, Sca-1 and CD117 in mouse bone marrow, Lin-ve and Lin+ve population. Bone marrow cells were isolated from C57/BL6J mouse and mononuclear population was separated from rest of the cell population. With the help of Magnetic associated cell sorter (MACS), Linve and Lin+ve cells were separated from the bone marrow. The expression of CD45, CD34, Sca1 and CD117 was evaluated in bone marrow, Lin-ve and Lin+ve population by flow cytometry. We found a significant increase in the expression of CD34 and CD117 in Lin-ve as compared to the bone marrow and Lin+ve population. These findings suggest that Lin-ve population has higher expression of stem cell progenitor markers and could be useful for tissue repair and regeneration.
authors: Neeru
authors: N
authors: Jindal
authors: Gillipsie
authors: G
authors: Minhas
authors: Sudesh
authors: S
authors: Prabhakar
authors: Neuroscience Research Lab, Department of Neurology, PGIMER, Chandigarh- 160012, India. akshay1anand@rediffmail.com.
authors: Akshay
authors: A
authors: Anand
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism*; D000950:Antigens, Ly / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism*; D005434:Flow Cytometry; D008280:Magnetics / Q000379:methods; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D000950:Antigens, Ly; C526827:Ly6 protein, mouse; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D019009:Proto-Oncogene Proteins c-kit