title: Association between haptoglobin and IgM levels and the clinical progression of caseous lymphadenitis in sheep.
abstract: BACKGROUND
  Sheep caseous lymphadenitis (CLA), caused by Corynebacterium pseudotuberculosis (Cp), is associated with direct economic losses and presents significant zoonotic potential. Despite the importance of the disease, a satisfactory vaccine model has not been developed. Thus, this study aimed to investigate the association between haptoglobin (Hp) and IgM levels and the clinical progression of CLA in primarily infected sheep and in sheep immunized with Cp- secreted antigens adjuvanted with Quillaja saponaria saponins. These animals were kept with CLA-positive sheep to simulate natural exposure that occurs in field conditions. During the experiment, the Hp and IgM levels were monitored for 21 days, and the development of internal CLA lesions was investigated through necropsies on day182 post-immunization.
  
  
  RESULTS
  Primarily infected sheep in Group 2 (inoculated with 2x105 Cp virulent strain) had higher Hp values between the first and ninth days post inoculation (PI) than sheep in Group 1 (control; P < 0.05). Immunized animals in Group 3 had significantly higher Hp values between the third and seventh days PI, compared with the control group (P < 0.01). Binary logistic regression (BLR) analysis of primarily infected sheep indicated an association between Hp concentration and CLA clinical progression: animals with high Hp values had 99.9% less risk of having CLA abscesses than animals with low Hp levels (Odds ratio = 0.001, P < 0.05). Both experimental groups had significantly higher IgM titers than the control group around the ninth and eleventh days PI (P < 0.05). The BLR analysis for immunized sheep indicated an association between IgM levels and clinical progression: sheep with high IgM titers had 100.0% less risk of having CLA abscesses than animals with low IgM levels (Odds ratio = 0.000, P < 0.05).
  
  
  CONCLUSIONS
  Resistance to C. pseudotuberculosis infection is supported by the early acute phase response, in which up-regulation of Hp and IgM were predictive of a lower risk of CLA lesion development. Because the immunogen used in this study induced a high production of both Hp and IgM, Q. saponaria saponin should be considered a promising candidate in vaccine formulations against sheep CLA.
authors: Bruno L
authors: BL
authors: Bastos
authors: Dan
authors: D
authors: Loureiro
authors: José T
authors: JT
authors: Raynal
authors: Maria T
authors: MT
authors: Guedes
authors: Vera Lúcia Costa
authors: VL
authors: Vale
authors: Lilia F
authors: LF
authors: Moura-Costa
authors: José E
authors: JE
authors: Guimarães
authors: Vasco
authors: V
authors: Azevedo
authors: Ricardo W
authors: RW
authors: Portela
authors: Laboratório de Imunologia e Biologia Molecular, Departamento de Biointeração, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Av, Reitor Miguel Calmon, S/N - Vale do Canela, Salvador, BA CEP 40140-100, Brazil. meyer.roberto@gmail.com.
authors: Roberto
authors: R
authors: Meyer
mesh_terms: D000818:Animals; D003354:Corynebacterium Infections / Q000097:blood / Q000382:microbiology / Q000662:veterinary*; D016925:Corynebacterium pseudotuberculosis; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D006242:Haptoglobins / Q000032:analysis*; D007075:Immunoglobulin M / Q000097:blood*; D008199:Lymphadenitis / Q000097:blood / Q000382:microbiology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000097:blood / Q000382:microbiology*
chemical_list: D006242:Haptoglobins; D007075:Immunoglobulin M