title: Effects of wenxin keli on the action potential and L-type calcium current in rats with transverse aortic constriction-induced heart failure.
abstract: Objective. We investigated the effects of WXKL on the action potential (AP) and the L-type calcium current (I Ca-L) in normal and hypertrophied myocytes. Methods. Forty male rats were randomly divided into two groups: the control group and the transverse aortic constriction- (TAC-) induced heart failure group. Cardiac hypertrophy was induced by TAC surgery, whereas the control group underwent a sham operation. Eight weeks after surgery, single cardiac ventricular myocytes were isolated from the hearts of the rats. The APs and I Ca-L were recorded using the whole-cell patch clamp technique. Results. The action potential duration (APD) of the TAC group was prolonged compared with the control group and was markedly shortened by WXKL treatment in a dose-dependent manner. The current densities of the I Ca-L in the TAC group treated with 5 g/L WXKL were significantly decreased compared with the TAC group. We also determined the effect of WXKL on the gating mechanism of the I Ca-L in the TAC group. We found that WXKL decreased the I Ca-L by accelerating the inactivation of the channels and delaying the recovery time from inactivation. Conclusions. The results suggest that WXKL affects the AP and blocked the I Ca-L, which ultimately resulted in the treatment of arrhythmias.
authors: Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China ; Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.
authors: Yu
authors: Y
authors: Chen
authors: Yang
authors: Y
authors: Li
authors: Lili
authors: L
authors: Guo
authors: Wen
authors: W
authors: Chen
authors: Mingjing
authors: M
authors: Zhao
authors: Yonghong
authors: Y
authors: Gao
authors: Aiming
authors: A
authors: Wu
authors: Lixia
authors: L
authors: Lou
authors: Jie
authors: J
authors: Wang
authors: Xiaoqiu
authors: X
authors: Liu
authors: Yanwei
authors: Y
authors: Xing