title: Composition of photosynthetic pigments in thylakoid membrane vesicles from spinach.
abstract: Thylakoid membranes from spinach were fragmented mechanically and separated into vesicles originating from grana and stroma-exposed lamellae (Andreasson et al. (1988) Biochim Biophys Acta 936: 339-350). The grana vesicles were further fragmented and separated into smaller vesicles originating from different parts of the grana (Svensson and Albertsson (1989) Photosynth Res 20: 249-259). All vesicles so obtained were analyzed with respect to chlorophyll and carotenoid composition by reverse phase HPLC. For all fractions the following relations (mole/mole) were found: 1 carotenoid per 4 chlorophyll (a+b), 2 lutein per 5 chlorophyll b and 5 violaxanthin per 100 chlorophyll (a + b). The contents of lutein and neoxanthin were each linearly related to chlorophyll b and β-carotene was linearly related to chlorophyll a.
authors: Institute of Biochemistry, Odense University, Campusvej 55, DK-5230, Odense M, Denmark.
authors: R K
authors: RK
authors: Juhler
authors: E
authors: E
authors: Andreasson
authors: S G
authors: SG
authors: Yu
authors: P K
authors: PK
authors: Albertsson