title: Characterization of novel plasmid-mediated β-lactamases (SHV-167 and ACT-16) associated with New Delhi metallo-β-lactamase-1 harbouring isolates from neonates in India.
abstract: Neonatal sepsis due to carbapenem-resistant bacteria is difficult to treat due to limited therapeutic options. The detection of the new carbapenemase New Delhi metallo-β-lactamase-1 (NDM-1) from neonates has further complicated the situation (Roy et al., 2011a). The potent metallo-β-lactamase NDM-1 efficiently hydrolyses all classes of β-lactam antibiotics (penicillins, cephalosporins and carbapenems) and is also associated with multiple determinants that enable the bacteria to become resistant to other antibiotic classes (Nordmann et al., 2011). In the presence of NDM-1 other β-lactamases may go unobserved because of the spectrum of activity of NDM-1 against all β-lactam antibiotics. Thus, under the canopy of the NDM-1 these β-lactamases also get the opportunity to spread. This communication reports association of two novel β-lactamases, SHV-type β-lactamase (SHV-167) and AmpC-type β-lactamase (ACT-16), in two NDM-1-carrying Enterobacteriaceae isolated from the blood of two septicaemic neonates admitted to a neonatal intensive care unit.
authors: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P33, CIT Road, Scheme XM, Beliaghata, Kolkata-700010, India.
authors: Saswati
authors: S
authors: Datta
authors: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P33, CIT Road, Scheme XM, Beliaghata, Kolkata-700010, India.
authors: Shravani
authors: S
authors: Mitra
authors: Department of Neonatology, Institute of Postgraduate Medical Education & Research, SSKM Hospital, Kolkata-700020, India.
authors: Rajlakshmi
authors: R
authors: Viswanathan
authors: Department of Neonatology, Institute of Postgraduate Medical Education & Research, SSKM Hospital, Kolkata-700020, India.
authors: Anindya
authors: A
authors: Saha
authors: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P33, CIT Road, Scheme XM, Beliaghata, Kolkata-700010, India.
authors: Sulagna
authors: S
authors: Basu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D015780:Carbapenems / Q000494:pharmacology; D052978:Disk Diffusion Antimicrobial Tests; D024881:Drug Resistance, Bacterial; D016972:Enterobacter cloacae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D006801:Humans; D007194:India; D007231:Infant, Newborn; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D018805:Sepsis; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; C048795:AmpC beta-lactamases; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1