title: The mitigating effect of Citrullus colocynthis (L.) fruit extract against genotoxicity induced by cyclophosphamide in mice bone marrow cells.
abstract: Possible genoprotective effect of Citrullus colocynthis (L.) (CCT) fruits extract against cyclophosphamide- (CP-)induced DNA damage in mice bone marrow cells was evaluated using micronucleus assay, as an index of induced chromosomal damage. Mice were preadministered with different doses of CCT via intraperitoneal injection for 7 consecutive days followed by injection with CP (70 mg/kg b.w.) 1 hr after the last injection of CCT. After 24 hr, mice were scarified to evaluate the frequency of micronucleated polychromatic erythrocytes (MnPCEs). In addition, the number of polychromatic erythrocytes (PCEs) among 1000 normochromatic erythrocytes (NCEs) per animal was recorded to evaluate bone marrow. Pretreatment with CCT significantly reduced the number of MnPCEs induced by CP in bone marrow cells (P < 0.0001). At 200 mg/kg, CCT had a maximum chemoprotective effect and reduced the number of MnPCEs by 6.37-fold and completely normalized the mitotic activity. CCT also led to marked proliferation and hypercellularity of immature myeloid elements after mice were treated with CP and mitigated the bone marrow suppression. Our study revealed that CCT has an antigenotoxic effect against CP-induced oxidative DNA damage in mice. Therefore, it could be used concomitantly as a supplement to protect people undergoing chemotherapy.
authors: Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, 18 Kilometer of Farah Abad Road, Sari, Iran ; Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari 48175-861, Iran.
authors: Mohammad
authors: M
authors: Shokrzadeh
authors: Aroona
authors: A
authors: Chabra
authors: Farshad
authors: F
authors: Naghshvar
authors: Amirhossein
authors: A
authors: Ahmadi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D029773:Citrullus / Q000737:chemistry*; D003520:Cyclophosphamide / Q000633:toxicity*; D005609:Free Radicals / Q000037:antagonists & inhibitors; D005638:Fruit / Q000737:chemistry*; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D009153:Mutagens / Q000633:toxicity; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D009153:Mutagens; D010936:Plant Extracts; D003520:Cyclophosphamide; D005978:Glutathione; D019803:Glutathione Disulfide