title: Molecular cloning of pea mRNAs encoding a shoot-specific polypeptide and light-induced polypeptides.
abstract: The molecular cloning of cDNA corresponds to pea seedling mRNA sequences encoding a shoot-specific polypeptide, the small subunit of the ribulose 1,5 biphosphate carboxylase and a component of the light-harvesting chlorophyll a/b complex is described. cDNA prepared from polysomal poly(A)RNA of light-grown shoots was enriched for shoot-specific and light-induced sequences by heterologous liquid hybridization with mercurated polysomal poly(A)RNA of dark-grown roots, followed by sulfhydryl chromatography. Cloned shoot-specific sequences were identified by 2D electrophoretic analysis of hybrid release translation products. The cloned shoot-specific sequence corresponded to a mRNA of 850 nt present both in light-and dark-grown shoots, and produced anin vitro translation product of Mr27 500 and isoelectric point of 4.7.
authors: Department of Developmental Plant Biology, Biological Centre, State University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands.
authors: S C
authors: SC
authors: De Vries
authors: M C
authors: MC
authors: Harmsen
authors: M T
authors: MT
authors: Kuiper
authors: H J
authors: HJ
authors: Dons
authors: J G
authors: JG
authors: Wessels