title: Expression, purification, crystallization and preliminary X-ray analysis of the receiver domain of Staphylococcus aureus LytR protein.
abstract: The response-regulatory protein LytR belongs to a family of transcription factors involved in the regulation of important virulence factors in pathogenic bacteria. The protein consists of a receiver domain and an effector domain, which play an important role in controlled cell death and lysis. The LytR receiver domain (LytR(N)) has been overexpressed, purified and crystallized using the sitting-drop and hanging-drop vapour-diffusion methods. The crystals grew as needles, with unit-cell parameters a = b = 84.82, c = 157.3 Å, α = β = 90, γ = 120°. LytR(N) crystallized in space group P6122 and the crystals diffracted to a maximum resolution of 2.34 Å. Based on the Matthews coefficient (V(M) = 5.44 Å(3) Da(-1)), one molecule is estimated to be present in the asymmetric unit.
authors: Department of Chemistry, York University, 4700 Keele Street, Toronto, ON M3J 1P3, Canada.
authors: Agnesa
authors: A
authors: Shala
authors: Kevin H
authors: KH
authors: Patel
authors: Dasantila
authors: D
authors: Golemi-Kotra
authors: Gerald F
authors: GF
authors: Audette
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013211:Staphylococcus aureus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C093500:LytR protein, Staphylococcus aureus; D011994:Recombinant Proteins; D014157:Transcription Factors
keywords: LytR receiver domain; Staphyloccocus aureus; transcription regulation