title: The olive mill wastewater as substrate for single cell oil production by Zygomycetes.
abstract: The conversion of olive mill wastewater (OMW) into high added value lipids containing polyunsaturated fatty acids (PUFA), in parallel with a significant phenolic removal by selected strains of Zygomycetes, is reported here for the first time. The growth of Mortierella isabellina, Mortierella ramanniana, Cunninghamella echinulata, Mucor sp., Thamnidium elegans and Zygorhynchus moelleri on solidified media was not significantly affected by the presence of OMW used in the growth medium up to 50% (v/v). Kinetic parameter values and conversion yields, estimated using a mathematical model which was fitted on the experimental data originated from submerged cultures, shows the ability of some Zygomycetes (i.e. T. elegans and Z. moelleri) to grow on OMW and accumulate storage material, i.e. lipids rich in PUFA, and these findings open new perspectives in OMW management and valorization. In liquid media containing OMW as sole carbon source, T. elegans and Z. moelleri produced 4.4 and 3.5g/L cell mass in surface (SC) and submerged (SMC) cultures, respectively, containing around 60% (w/w) of lipids. Oleic and palmitic acids were the predominant fatty acids. Gamma-linolenic acid was found in high percentages (up to 17.7%, w/w) in the lipid of Z. moelleri, in SMC with OMW as sole carbon source, while PUFA biosynthesis was not favored in SC.
authors: Unit of Microbiology, Division of Genetics, Cell and Development Biology, Department of Biology, University of Patras, Patras, Greece.
authors: Stamatia
authors: S
authors: Bellou
authors: Unit of Microbiology, Division of Genetics, Cell and Development Biology, Department of Biology, University of Patras, Patras, Greece.
authors: Anna
authors: A
authors: Makri
authors: Laboratory of Food Microbiology & Biotechnology, Department of Food Science and Technology, Agricultural University of Athens, Greece.
authors: Dimitrios
authors: D
authors: Sarris
authors: Unit of Microbiology, Division of Genetics, Cell and Development Biology, Department of Biology, University of Patras, Patras, Greece.
authors: Konstantinos
authors: K
authors: Michos
authors: Unit of Microbiology, Division of Genetics, Cell and Development Biology, Department of Biology, University of Patras, Patras, Greece.
authors: Penelope
authors: P
authors: Rentoumi
authors: Department of Chemistry, Gebze Institute of Technology, Gebze-Kocaeli, Turkey.
authors: Ayhan
authors: A
authors: Celik
authors: Laboratory of Food Microbiology & Biotechnology, Department of Food Science and Technology, Agricultural University of Athens, Greece.
authors: Seraphim
authors: S
authors: Papanikolaou
authors: Unit of Microbiology, Division of Genetics, Cell and Development Biology, Department of Biology, University of Patras, Patras, Greece; Department of Biological Sciences, King Abdulaziz University, Jeddah, Saudi Arabia. Electronic address: George.Aggelis@upatras.gr.
authors: George
authors: G
authors: Aggelis
mesh_terms: D003470:Culture Media; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D005658:Fungi / Q000145:classification / Q000254:growth & development*; D031658:Olea / Q000378:metabolism*; D019301:Oleic Acid / Q000378:metabolism; D019308:Palmitic Acid / Q000378:metabolism; D062065:Wastewater / Q000737:chemistry* / Q000382:microbiology; D017965:gamma-Linolenic Acid / Q000096:biosynthesis
chemical_list: D003470:Culture Media; D005231:Fatty Acids, Unsaturated; D019301:Oleic Acid; D019308:Palmitic Acid; D062065:Wastewater; D017965:gamma-Linolenic Acid
keywords: Modeling; Mortierella isabellina, Mortierella ramanniana, Cunninghamella echinulata, Mucor sp., Thamnidium elegans and Zygorhynchus moelleri; Olive mill wastewater; Polyunsaturated fatty acids; Surface and submerged cultures