title: Evaluation of anti-A/Udorn/307/1972 antibody specificity to influenza A/H3N2 viruses using an evanescent-field coupled waveguide-mode sensor.
abstract: Discrimination of closely related strains is a key issue, particularly for infectious diseases whose incidence fluctuates according to variations in the season and evolutionary changes. Among infectious diseases, influenza viral infections are a worldwide cause of pandemic disease and mortality. With the emergence of different influenza strains, it is vital to develop a method using antibodies that can differentiate between viral types and subtypes. Ideally, such a system would also be user friendly. In this study, a polyclonal antibody generated against A/Udorn/307/1972 (H3N2) was used as a probe to distinguish between influenza H3N2 viruses based on the interaction between the antibody and hemagglutinin, demonstrating its applicability for viral discrimination. Clear discrimination was demonstrated using an evanescent-field-coupled waveguide-mode sensor, which has appealing characteristics over other methods in the viewpoint of improving the sensitivity, measurement time, portability and usability. Further supporting evidence was obtained using enzyme-linked immunosorbent assays, hemagglutination-inhibition assays, and infectivity neutralization assays. The results obtained indicate that the polyclonal antibody used here is a potential probe for distinguishing influenza viruses and, with the aid of a handheld sensor it could be used for influenza surveillance.
authors: Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, Central 5, Tsukuba, Ibaraki, Japan.
authors: Subash C B
authors: SC
authors: Gopinath
authors: Koichi
authors: K
authors: Awazu
authors: Makoto
authors: M
authors: Fujimaki
authors: Kazufumi
authors: K
authors: Shimizu
mesh_terms: D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000737:chemistry / Q000276:immunology*; D000918:Antibody Specificity; D015374:Biosensing Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D006046:Gold / Q000737:chemistry; D006385:Hemagglutination Inhibition Tests; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology* / Q000302:isolation & purification*; D053768:Metal Nanoparticles / Q000737:chemistry; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D013045:Species Specificity
chemical_list: D000914:Antibodies, Viral; D006046:Gold