title: Molecular diagnosis of an ocular toxocariasis patient in Vietnam.
abstract: An ocular Toxocara canis infection is reported for the first time in Vietnam. A 34-year-old man residing in a village of Son La Province, North Vietnam, visited the National Eye Hospital (NEH) in August 2011. He felt a bulge-sticking pain in his left eye and loss of vision occurred over 3 months before visiting the hospital. The eye examination in the hospital showed damage of the left eye, red eye, retinal fibrosis, retinal detachment, inflammation of the eye tissues, retinal granulomas, and a parasitic cyst inside. A larva of Toxocara was collected with the cyst by a medical doctor by surgery. Comparison of 264 nucleotides of internal transcribed spacer 2 (ITS2) of ribosomal DNA was done between our Vietnamese Toxocara canis and other Toxocara geographical isolates, including Chinese T. canis, Japanese T. canis, Sri Lankan T. canis, and Iranian T. canis. The nucleotide homology was 97-99%, when our T. canis was compared with geographical isolates. Identification of a T. canis infection in the eye by a molecular method was performed for the first time in Vietnam.
authors: Department of Parasitology, Hanoi Medical University, Hanoi, Vietnam.
authors: Nguyen
authors: N
authors: Van De
authors: Nguyen Vu
authors: NV
authors: Trung
authors: Le Van
authors: le V
authors: Duyet
authors: Jong-Yil
authors: JY
authors: Chai
mesh_terms: D000328:Adult; D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D015822:Eye Infections, Parasitic / Q000175:diagnosis* / Q000469:parasitology; D006801:Humans; D007814:Larva; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017166:Toxocara canis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014120:Toxocariasis / Q000175:diagnosis* / Q000469:parasitology; D014744:Vietnam
chemical_list: D018509:DNA, Helminth; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer
keywords: ITS2; PCR; Toxocara canis; Vietnam; case report; eye; molecular diagnosis