title: Cobalt chloride induced stimulation of Photosystem II electron transport in Synechocystis sp. PCC 6803 cells.
abstract: Synechocystis sp. PCC 6803 when grown in the presence of sublethal (μM) levels of cobalt chloride shows an enhancement of Photosystem II (PS II) catalyzed Hill reaction. This stimulation seems to be induced by cobalt ions as other metal ions inhibit para-benzoquinone catalyzed Hill reaction. At saturating white light intensity, this enhancement is two times over that of the control cells on unit chlorophyll basis. Analysis of the PS II electron transport rate at varying intensities of white, blue or yellow light suggests an increased maximal rates but no change in the quantum yield or effective antenna size of CoCl2-grown cells. There were no structural and functional changes in the phycobilisome as judged by the absence of changes in the phycocyanin/allophycocyanin ratio, fluorescence emission spectra, second derivative absorption spectra at 77 K and SDS-PAGE analysis of isolated phycobilisomes. The 77 K fluorescence emission spectra of the cells showed a decrease in the ratio of Photosystem I emission (F725) to Photosystem II emission (F685) in CoCl2-grown cells compared to the control cells. These observations indicate three possibilities: (1) there is an increase in the number of Photosystem II units; (2) a faster turnover of Photosystem II centers; or (3) an alteration in energy redistribution between PS II and PS I in CoCl2-grown cells which causes stimulation of Photosystem II electron transport rate.
authors: School of Life Sciences, Jawaharlal Nehru University, New Mehrauli Road, 110067, New Delhi, India.
authors: S
authors: S
authors: Tiwari
authors: P
authors: P
authors: Mohanty