title: Structure of the uracil complex of Vaccinia virus uracil DNA glycosylase.
abstract: Poxvirus uracil DNA glycosylases are the most diverse members of the family I uracil DNA glycosylases (UNGs). The crystal structure of the uracil complex of Vaccinia virus uracil DNA glycosylase (D4) was determined at 2.03 Å resolution. One uracil molecule was located in the active-site pocket in each of the 12 noncrystallographic symmetry-related D4 subunits. Although the UNGs of the poxviruses (including D4) feature significant differences in the characteristic motifs designated for uracil recognition and in the base-excision mechanism, the architecture of the active-site pocket in D4 is very similar to that in UNGs of other organisms. Overall, the interactions of the bound uracil with the active-site residues are also similar to the interactions previously observed in the structures of human and Escherichia coli UNG.
authors: Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
authors: N
authors: N
authors: Schormann
authors: S
authors: S
authors: Banerjee
authors: R
authors: R
authors: Ricciardi
authors: D
authors: D
authors: Chattopadhyay
mesh_terms: D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008958:Models, Molecular; D054730:Protein Interaction Domains and Motifs; D017433:Protein Structure, Secondary; D021122:Protein Subunits / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D040681:Structural Homology, Protein; D014498:Uracil / Q000737:chemistry* / Q000378:metabolism; D051981:Uracil-DNA Glycosidase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014616:Vaccinia virus / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D021122:Protein Subunits; D011994:Recombinant Proteins; D014764:Viral Proteins; D014498:Uracil; D051981:Uracil-DNA Glycosidase
keywords: Vaccinia virus; uracil DNA glycosidase