title: Mitochondrial oxidative energy metabolism in guanethidine-induced sympathectomized ducklings.
abstract: Here we investigate the possible involvement of the sympathetic nervous system in the respiratory properties of intermyofibrillar and subsarcolemmal mitochondrial populations from heart and gastrocnemius muscles. Mitochondrial oxidative phosphorylation was assessed polarographically by using succinate (plus rotenone), and ascorbate plus N,N,N',N'-tetramethyl-p-phenyl-enediamine (plus antimycin) as respiratory substrates. We report that chronic chemical sympathectomy with guanethidine (150 mg/kg, daily for 3 weeks) induced a marked decrease in whole body metabolic and heart rates, in plasma metabolites (fatty acids and glucose) and norepinephrine levels. Guanethidine treatment decreased mainly the oxidative phosphorylation capacity of subsarcolemmal mitochondria in heart, irrespective of the substrate used. In contrast, both mitochondrial populations were affected by the treatment in skeletal muscle. This suggests that sympathetic nervous system activity can alter the energetic status of muscle cells, and to some extent play a thermogenic role in birds.
authors: Université Moulay Ismail de Meknès, EPPE-FST, Errachidia, Morocco. filali.younes@gmail.com.
authors: Younes
authors: Y
authors: Filali-Zegzouti
authors: Jean-Louis
authors: JL
authors: Rouanet
authors: Toufiq
authors: T
authors: Fechtali
authors: Damien
authors: D
authors: Roussel
mesh_terms: D000818:Animals; D000968:Antimycin A / Q000031:analogs & derivatives / Q000737:chemistry; D001205:Ascorbic Acid / Q000737:chemistry; D001835:Body Weight; D002395:Catecholamines / Q000737:chemistry; D004372:Ducks; D005227:Fatty Acids / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006145:Guanethidine / Q000737:chemistry*; D006339:Heart Rate; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010100:Oxygen / Q000737:chemistry*; D012402:Rotenone / Q000737:chemistry; D012508:Sarcolemma / Q000378:metabolism; D019802:Succinic Acid / Q000737:chemistry; D013772:Tetramethylphenylenediamine / Q000737:chemistry; D014475:Uncoupling Agents / Q000737:chemistry
chemical_list: D002395:Catecholamines; D005227:Fatty Acids; D014475:Uncoupling Agents; D012402:Rotenone; C032456:antimycin; D000968:Antimycin A; D019802:Succinic Acid; D005947:Glucose; D013772:Tetramethylphenylenediamine; D001205:Ascorbic Acid; D010100:Oxygen; D006145:Guanethidine