title: Expression of the Tpanxb1 gene from Taenia pisiformis and its potential diagnostic value by dot-ELISA.
abstract: Cysticercosis, caused by the larvae of Taenia pisiformis, is a common disease in rabbits that results in economic losses. To date, there has been limited information available on the early detection of infection by this parasite. This study describes a dot-ELISA method based on an autologous antigen annexin B1 (Tpanxb1). Its potential for serodiagnosis of rabbit cysticercosis was also evaluated. Western blot analysis revealed that the recombinant Tpanxb1 (rTpanxb1) protein could be specifically recognized by rabbit anti-sera. In serum trials, the antibodies could be detected by dot-ELISA using rTpanxb1 at 14 days post-infection. The positive response was present for up to 49 days post-infection. Based on the necropsy results of 169 rabbit samples, the relative sensitivity and specificity of the dot-ELISA were 94.55% and 92.86%, respectively. This study provides a foundation for studying the immunological function of annexin and its application to control Taenia cestodes.
authors: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, Sichuan Province, China;
authors: Deying
authors: D
authors: Yang
authors: Lin
authors: L
authors: Chen
authors: Xuhang
authors: X
authors: Wu
authors: Xuan
authors: X
authors: Zhou
authors: Mei
authors: M
authors: Li
authors: Zuqin
authors: Z
authors: Chen
authors: Xiang
authors: X
authors: Nong
authors: Xiaobin
authors: X
authors: Gu
authors: Xuerong
authors: X
authors: Peng
authors: Guangyou
authors: G
authors: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017302:Annexins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D003551:Cysticercosis / Q000175:diagnosis / Q000662:veterinary*; D018076:DNA, Complementary / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005786:Gene Expression Regulation; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting / Q000662:veterinary; D011817:Rabbits / Q000469:parasitology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D012680:Sensitivity and Specificity; D013621:Taenia / Q000235:genetics* / Q000276:immunology
chemical_list: D017302:Annexins; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018076:DNA, Complementary; D015801:Helminth Proteins; D007106:Immune Sera; D011994:Recombinant Proteins