title: The actions of neuropeptide SF on the hypothalamic-pituitary-adrenal axis and behavior in rats.
abstract: Present experiments focused on measuring the effect of neuropeptide SF (NPSF) on the hypothalamus-pituitary-adrenal (HPA) axis and behavior. The peptide was administered in different doses (0.25, 0.5, 1, 2 μg) intracerebroventricularly to rats, and the behavior of which was then observed by telemetry and open-field test. Effect of NPSF on core temperature was also measured via telemetry. Plasma ACTH and corticosterone concentrations were measured to assess the influence of NPSF on the HPA activation. In addition, the changes in corticotrophin-releasing hormone (CRH) level in the hypothalamic paraventricular nucleus were continuously monitored by means of intracerebral microdialysis. Our results showed that NPSF augmented paraventricular CRH release and increased ACTH and corticosterone levels in the plasma. The release of corticosterone was successfully blocked by the pre-treatment of the CRH antagonist α-helical CRH9-41. Spontaneous and exploratory locomotor activity was also stimulated according to the telemetric and open-field studies. However, NPSF only tended to alter stereotyped behavior in the open-field experiments. These results demonstrate that NPSF may play a physiologic role in the regulation of such circadian functions as the activity of motor centers and the HPA axis, through the release of CRH.
authors: Department of Pathophysiology, University of Szeged, Szeged, Hungary.
authors: Miklós
authors: M
authors: Jászberényi
authors: Department of Pathophysiology, University of Szeged, Szeged, Hungary.
authors: Zsolt
authors: Z
authors: Bagosi
authors: Department of Pathophysiology, University of Szeged, Szeged, Hungary.
authors: Krisztina
authors: K
authors: Csabafi
authors: Department of Pathophysiology, University of Szeged, Szeged, Hungary.
authors: Miklós
authors: M
authors: Palotai
authors: Department of Pathophysiology, University of Szeged, Szeged, Hungary; MTA-SZTE Neuroscience Research Group of the Hungarian Academy of Sciences Szeged, Szeged, Hungary. Electronic address: telegdy.gyula@med.u-szeged.hu.
authors: Gyula
authors: G
authors: Telegdy
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D001833:Body Temperature Regulation; D003345:Corticosterone / Q000097:blood; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism; D004609:Eliminative Behavior, Animal; D007030:Hypothalamo-Hypophyseal System / Q000378:metabolism*; D008124:Locomotion; D008297:Male; D009479:Neuropeptides / Q000494:pharmacology / Q000502:physiology*; D010913:Pituitary-Adrenal System / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D009479:Neuropeptides; C414382:neuropeptide SF; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone; D003345:Corticosterone
keywords: Hypothalamic–pituitary–adrenal system; Neuropeptide SF; Open-field; Telemetry