title: Protective effect of aquacultured flounder fish-derived peptide against oxidative stress in zebrafish.
abstract: This study investigates the protective effect of aquacultured flounder fish-derived peptide (AFFP) against 2,2-azobis-(2-amidinopropane) hydrochloride (AAPH)-induced oxidative damage in a zebrafish model. Zebrafish embryos were evaluated for the protective effect by heartbeat rate, survival rate, ROS generation, lipid peroxidation, and cell death. In the results, the AAPH group showed a low survival rate, whereas the AFFP and AAPH co-treated group increased a survival rate. Also, AFFP dose-dependently reduced AAPH-induced intracellular ROS and lipid peroxidation, and decreased cell death in AAPH-induced zebrafish. These results revealed that AFFP could be used as a natural antioxidant, and that the zebrafish provides an alternative in vivo model to efficiently evaluate the antioxidative effects of peptides on fishes.
authors: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Ju-Young
authors: JY
authors: Ko
authors: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Eun-A
authors: EA
authors: Kim
authors: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Ji-Hyeok
authors: JH
authors: Lee
authors: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Min-Cheol
authors: MC
authors: Kang
authors: Industry-Academy Cooperation Foundation, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Jung-Suck
authors: JS
authors: Lee
authors: Department of Seafood Science and Technology, Institute of Marine Industry, Gyeongsang National University, Tongyeong 650-160, Republic of Korea.
authors: Jin-Soo
authors: JS
authors: Kim
authors: Department of Biomedical Engineering, and Center for Marine-Integrated Biomedical Technology (BK21 Plus) Pukyong National University, Busan 608-737, Republic of Korea. Electronic address: wkjung@pknu.ac.kr.
authors: Won-Kyo
authors: WK
authors: Jung
authors: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: youjinj@jejunu.ac.kr.
authors: You-Jin
authors: YJ
authors: Jeon
mesh_terms: D000578:Amidines / Q000008:administration & dosage; D000818:Animals; D016923:Cell Death / Q000276:immunology; D006339:Heart Rate / Q000276:immunology; D015227:Lipid Peroxidation / Q000276:immunology; D018384:Oxidative Stress / Q000276:immunology*; D010455:Peptides / Q000494:pharmacology*; D011897:Random Allocation; D017382:Reactive Oxygen Species / Q000276:immunology*; D013050:Spectrometry, Fluorescence; D015027:Zebrafish / Q000276:immunology*
chemical_list: D000578:Amidines; D010455:Peptides; D017382:Reactive Oxygen Species; C046728:2,2'-azobis(2-amidinopropane)
keywords: AAPH; Antioxidative peptide; In vivo model; Oxidative stress; Zebrafish