title: Use of bacteriophage particles displaying influenza virus hemagglutinin for the detection of hemagglutination-inhibition antibodies.
abstract: Bacteriophage lambda capsids provide a flexible molecular scaffold that can be engineered to display a wide range of exogenous proteins, including full-length viral glycoproteins produced in eukaryotic cells. One application for such particles lies in the detection of virus-specific antibodies, since they may obviate the need to work with infectious stocks of highly pathogenic or emerging viruses that can pose significant biosafety and biocontainment challenges. Bacteriophage lambda capsids were produced that displayed an insect-cell derived, recombinant H5 influenza virus hemagglutinin (HA) on their surface. The particles agglutinated red blood cells efficiently, in a manner that could be blocked using H5 HA-specific monoclonal antibodies. The particles were then used to develop a modified hemagglutinination-inhibition (HAI) assay, which successfully identified human sera with H5 HA-specific HAI activity. These results demonstrate the utility of HA-displaying bacteriophage capsids for the detection of influenza virus-specific HAI antibodies.
authors: Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester 14642, NY, USA.
authors: William
authors: W
authors: Domm
authors: Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester 14642, NY, USA.
authors: Matthew
authors: M
authors: Brewer
authors: Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester 14642, NY, USA.
authors: Steven F
authors: SF
authors: Baker
authors: Department of Biostatistics and Computational Biology, University of Rochester Medical Center, Rochester 14642, NY, USA.
authors: Changyong
authors: C
authors: Feng
authors: Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester 14642, NY, USA.
authors: Luis
authors: L
authors: Martínez-Sobrido
authors: Department of Medicine (Infectious Diseases Division), University of Rochester Medical Center, Rochester 14642, NY, USA.
authors: John
authors: J
authors: Treanor
authors: Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester 14642, NY, USA. Electronic address: stephen_dewhurst@urmc.rochester.edu.
authors: Stephen
authors: S
authors: Dewhurst
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D010582:Bacteriophage lambda / Q000235:genetics*; D006385:Hemagglutination Inhibition Tests / Q000379:methods; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007251:Influenza, Human / Q000175:diagnosis*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011994:Recombinant Proteins; C109963:hemagglutinin, avian influenza A virus
keywords: Bacteriophage; H5 HA; Hemagglutination inhibition; Hemagglutinin; Influenza virus; Lambda