title: High performance liquid chromatographic analysis of almotriptan malate in bulk and tablets.
abstract: OBJECTIVE
  A simple RP-HPLC method has been developed and validated for the determination of almotriptan malate (ATM) in bulk and tablets.
  
  
  METHODS
  Chromatographic separation of ATM was achieved by using a Thermo Scientific C18 column. A Mobile phase containing a mixture of methanol, water and acetic acid (4:8:0.1 v/v) was pumped at the flow rate of 1 mL/min. Detection was performed at 227 nm. According to ICH guidelines, the method was validated.
  
  
  RESULTS
  The calibration curve was linear in the concentration range 5-60 µg/mL for the ATM with regression coefficient 0.9999. The method was precise with RSD <1.2%. Excellent recoveries of 99.60 - 100.80% proved the accuracy of the method. The limits of detection and quantification were found to be 0.025 and 0.075 µg/mL, respectively.
  
  
  CONCLUSIONS
  The method was successfully applied for the quantification of ATM in tablets with acceptable accuracy and precision.
authors: Department of Pharmaceutical Biotechnology, Vishnu Institute of Pharmaceutical Education and Research, Narsapur, Andhra Pradesh-500072.
authors: Petikam
authors: P
authors: Lavudu
authors: Avula Prameela
authors: AP
authors: Rani
authors: Chepuri
authors: C
authors: Divya
authors: Chandra Bala
authors: CB
authors: Sekharan
keywords: Almotriptan Malate; Analysis; HPLC; Tablets; Validation