title: Visual detection of microRNA with lateral flow nucleic acid biosensor.
abstract: We report a DNA-gold nanoparticle (DNA-GNP) based lateral flow nucleic acid biosensor for visual detection of microRNA (miRNA)-215 in aqueous solutions and biological samples with low-cost and short analysis time. Sandwich-type hybridization reactions among GNP-labeled DNA probe, miRNA-215 and biotin-modified DNA probes were performed on the lateral flow device. The accumulation of GNPs on the test zone of the biosensor enables the visual detection of miRNA-215. After systematic optimization, the biosensor was able to detect a minimum concentration of 60 pM miRNA-215. The biosensor was applied to detect miRNA-215 from A549 cell lysate directly without complex sample treatment, and the detection limit of 0.148 million cells was obtained. This study provides a simple, rapid, specific and low-cost approach for miRNA detection in aqueous solutions and biological samples, showing great promise for clinical application and biomedical diagnosis in some malignant diseases.
authors: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing 100083, PR China; Department of Chemistry and Biochemistry, North Dakota State University, Fargo, ND 58105, USA.
authors: Xuefei
authors: X
authors: Gao
authors: Department of Chemistry and Biochemistry, North Dakota State University, Fargo, ND 58105, USA.
authors: Hui
authors: H
authors: Xu
authors: Department of Chemistry and Biochemistry, North Dakota State University, Fargo, ND 58105, USA.
authors: Meenu
authors: M
authors: Baloda
authors: Department of Chemistry and Biochemistry, North Dakota State University, Fargo, ND 58105, USA.
authors: Anant S
authors: AS
authors: Gurung
authors: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing 100083, PR China.
authors: Li-Ping
authors: LP
authors: Xu
authors: Department of Chemistry and Biochemistry, North Dakota State University, Fargo, ND 58105, USA.
authors: Tao
authors: T
authors: Wang
authors: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing 100083, PR China. Electronic address: zhangxueji@ustb.edu.cn.
authors: Xueji
authors: X
authors: Zhang
authors: Department of Chemistry and Biochemistry, North Dakota State University, Fargo, ND 58105, USA; College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 35002, PR China. Electronic address: guodong.liu@ndsu.edu.
authors: Guodong
authors: G
authors: Liu
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D002460:Cell Line; D015342:DNA Probes / Q000737:chemistry*; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D035683:MicroRNAs / Q000032:analysis*; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D015342:DNA Probes; C553667:MIRN215 microRNA, human; D035683:MicroRNAs; D006046:Gold
keywords: Lateral flow biosensor; MicroRNA; Nucleic acid; Visual detection