title: Synthesis, characterization, DNA binding, cleavage activity and cytotoxicity of copper(II) complexes.
abstract: Three new mononuclear copper(II) complexes, [Cu(L2)](2+) (1), [Cu(acac)(L)](+) (2), and [Cu(acac-Cl)(L)](+) (3) (L = 2-(4-pyridine)oxazo[4,5-f]1,10-phenanthroline (4-PDOP); acac = acetylacetone; acac-Cl = 3-chloroacetylacetone), have been synthesized and characterized by elemental analysis, high resolution mass spectrometry (Q-TOF), and IR spectroscopy. Two of the complexes were structurally characterized by single-crystal X-ray diffraction techniques. Their interactions with DNA were studied by UV-vis absorption and emission spectra, viscosity, thermal melting, DNA unwinding assay and CD spectroscopy. The nucleolytic cleavage activity of the compounds was carried out on double stranded pBR322 circular plasmid DNA by using a gel electrophoresis experiment in the presence and absence of an oxidant (H2O2). Active oxygen intermediates such as hydroxyl radicals and hydrogen peroxide generated in the presence of L and complexes 1-3 may act as active species for the DNA scission. The cytotoxicity of the complexes against HepG2 cancer cells was also studied.
authors: Key Laboratory of Analysis and Detection Technology for Food Safety (Ministry of Education and Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350108, P. R. China. mjli@fzu.edu.cn yichq@mail.sysu.edu.cn.
authors: Mei-Jin
authors: MJ
authors: Li
authors: Tao-Yu
authors: TY
authors: Lan
authors: Xiu-Hui
authors: XH
authors: Cao
authors: Huang-Hao
authors: HH
authors: Yang
authors: Yupeng
authors: Y
authors: Shi
authors: Changqing
authors: C
authors: Yi
authors: Guo-Nan
authors: GN
authors: Chen
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D002417:Cattle; D060326:Chemistry Techniques, Synthetic; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D003300:Copper / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D053837:DNA Cleavage / Q000187:drug effects*; D004563:Electrochemistry; D004996:Ethidium / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D016166:Free Radical Scavengers; D003300:Copper; D004247:DNA; D004996:Ethidium