title: [Effect of plant extracts on cytotoxic activity of Vibrio cholerae hemolysin].
abstract: OBJECTIVE
  Study of plant extracts that have the ability to neutralize cytotoxic activity of hemolysin.
  
  
  METHODS
  Preparations of purified and recombinant V. cholerae eltor hemolysin as well as supernatants of V. cholerae strains were used. Determination ofcytotoxic activity of hemolysin and neutralizing activity of plant extracts were carried out by using cell cultures CHO-K1 and CaCo2.
  
  
  RESULTS
  Out of 9 water extracts only 3 - extracts of Rhei rhizome, Limonium gmelinii and Quercus robur neutralized hemolysin in cell culture CHO-K1 and CaCo2, whereas the other extracts--Humulus lupulus, Ocimum basilicum, Chelidonium majus, Juglans regia, Achillea milefolium and Hypericum perforatum did not have anti-cytotoxic effect. Neutralizing properties of extracts are exhibited during their co-incubation with hemolysin preparations and supernatants of V. cholerae strains already within 10 minutes.
  
  
  CONCLUSIONS
  Plant extracts that have anti-cytotoxic activity against hemolysin are perspective for development oftherapeutic-prophylaxis preparations.
authors: O V
authors: OV
authors: Markina
authors: L P
authors: LP
authors: Alekseeva
authors: N V
authors: NV
authors: Markin
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000187:drug effects* / Q000633:toxicity; D016466:CHO Cells; D018938:Caco-2 Cells; D002771:Cholera / Q000188:drug therapy* / Q000382:microbiology; D002772:Cholera Toxin / Q000037:antagonists & inhibitors / Q000737:chemistry; D006224:Cricetinae; D003412:Cricetulus; D006460:Hemolysin Proteins / Q000187:drug effects* / Q000633:toxicity; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D029606:Plumbaginaceae / Q000737:chemistry; D029963:Quercus / Q000737:chemistry; D014734:Vibrio cholerae / Q000187:drug effects / Q000472:pathogenicity
chemical_list: D001426:Bacterial Proteins; D006460:Hemolysin Proteins; D010936:Plant Extracts; C073845:hemolysin, Vibrio; D002772:Cholera Toxin