title: Transient dual-luciferase assay combined with a glucocorticoid-inducible system for rice protoplasts.
abstract: We describe a transient dual-luciferase assay combined with a glucocorticoid-inducible system for rice protoplasts. Luciferase genes were efficiently induced by adding 0.1 µM of dexamethasone to the protoplast suspension, the activity of the luciferases reaching a maximum 6 h after induction. This assay system is applicable to studying the translation efficiency of rice by using the luciferase gene harboring tandem copies of an interesting codon at the 5' end.
authors: Graduate School of Agriculture, Kyoto University.
authors: Yuki
authors: Y
authors: Hamamoto
authors: Takuji
authors: T
authors: Tsukiyama
authors: Yoshihiro
authors: Y
authors: Yoshikake
authors: Masayoshi
authors: M
authors: Teraishi
authors: Yutaka
authors: Y
authors: Okumoto
authors: Takatoshi
authors: T
authors: Tanisaka
mesh_terms: D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology*; D017930:Genes, Reporter / Q000235:genetics; D005821:Genetic Techniques; D005938:Glucocorticoids / Q000494:pharmacology*; D008156:Luciferases / Q000235:genetics*; D012275:Oryza / Q000166:cytology*; D011523:Protoplasts / Q000187:drug effects* / Q000378:metabolism
chemical_list: D005938:Glucocorticoids; D003907:Dexamethasone; D008156:Luciferases