title: A novel small molecule that induces oxidative stress and selectively kills malignant cells.
abstract: We have synthesized a novel molecule named XB05 (1-bromo-1,1-difluoro-non-2-yn-4-ol) and evaluated its effects in a variety of human cell lines. XB05 displayed potent antiproliferative activity against cell lines derived from leukemia or solid tumors, but had less effect on nonmalignant cells. To identify factors that contribute to the cancer selectivity of XB05, we chose three cell lines that had high sensitivity to XB05 (U937 leukemia), moderate sensitivity (A549 lung cancer), or low sensitivity (Hs27 nonmalignant skin fibroblasts), and proceeded to assess cell death and oxidative stress in these cells. XB05 was found to induce cell death via both apoptotic and nonapoptotic mechanisms in U937 and A549 cells, whereas it had no cytotoxicity against Hs27 cells at comparable concentrations. Treatment with XB05 caused an increase in reactive oxygen species in all cell lines tested, but levels were higher in malignant compared to nonmalignant cells. XB05 treatment also induced DNA damage exclusively in the malignant cells. Differences in antioxidant responses were observed between cell lines. For example, XB05 caused a decrease in levels of glutathione and nuclear Nrf2 in the most sensitive cells (U937), whereas the least sensitive cells (Hs27) displayed increased glutathione levels and no change in nuclear Nrf2. XB05 could react in vitro with cysteine and glutathione, but had much lower reactivity compared to typical thiol-reactive electrophiles, diethyl maleate and maleimide. In summary, XB05 is a novel compound that selectively kills malignant cells, most likely by disrupting cellular redox homeostasis, making it a promising candidate for development as a chemotherapeutic agent.
authors: Department of Biochemistry and Molecular Biology, University of Louisville, Louisville, KY 40202, USA; Molecular Targets Program of the James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA.
authors: Francesca R
authors: FR
authors: Šalipur
authors: Molecular Targets Program of the James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA; Department of Medicine, University of Louisville, Louisville, KY 40202, USA.
authors: E Merit
authors: EM
authors: Reyes-Reyes
authors: Department of Chemistry, University of Louisville, Louisville, KY 40202, USA.
authors: Bo
authors: B
authors: Xu
authors: Department of Chemistry, University of Louisville, Louisville, KY 40202, USA.
authors: Gerald B
authors: GB
authors: Hammond
authors: Department of Biochemistry and Molecular Biology, University of Louisville, Louisville, KY 40202, USA; Molecular Targets Program of the James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA; Department of Medicine, University of Louisville, Louisville, KY 40202, USA. Electronic address: paula.bates@louisville.edu.
authors: Paula J
authors: PJ
authors: Bates
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D005233:Fatty Alcohols / Q000737:chemistry*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D006842:Hydrocarbons, Brominated / Q000737:chemistry*; D008175:Lung Neoplasms / Q000188:drug therapy; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: C000592486:1-bromo-1,1-difluoronon-2-yn-4-ol; D000975:Antioxidants; D005233:Fatty Alcohols; D006842:Hydrocarbons, Brominated; D017382:Reactive Oxygen Species; D005978:Glutathione
keywords: Cancer; Cell death; Glutathione; Leukemia; Nrf2; Oxidative stress; XB05