title: Comparison of single 1-day-old chick vaccination using a Newcastle disease virus vector with a prime/boost vaccination scheme against a highly pathogenic avian influenza H5N1 challenge.
abstract: Avian influenza (AI) vaccines should be used as part of a whole comprehensive AI control programme. Vectored vaccines based on Newcastle disease virus (NDV) are very promising, but are so far licensed in only a few countries. In the present study, the immunogenicity and protection against a highly pathogenic H5N1 influenza challenge were evaluated after vaccination with an enterotropic NDV vector expressing an H5 haemagglutinin (rNDV-H5) in 1-day-old specific pathogen free chickens inoculated once, twice or once followed by a heterologous boost with an inactivated H5N9 vaccine (iH5N9). The heterologous prime/boost rNDV-H5/iH5N9 combination afforded the best level of protection against the H5N1 challenge performed at 6 weeks of age. Two rNDV-H5 administrations conferred a good level of protection after challenge, although only a cellular H5-specific response could be detected. Interestingly, a single administration of rNDV-H5 gave the same level of protection as the double administration but without any detectable H5-specific immune response. In contrast to AI immunity, a high humoral, mucosal and cellular NDV-specific immunity could be detected up to 6 weeks post vaccination after using the three different vaccination schedules. NDV-specific mucosal and cellular immune responses were slightly higher after double rNDV-H5 vaccination when compared with single inoculation. Finally, the heterologous prime/boost rNDV-H5/iH5N9 combination induced a broader detectable immunity including systemic, mucosal and cellular AI and NDV-specific responses.
authors: a FZEA-USP , Av. Duque de Caxias Norte, 225, Pirassununga - SP, CEP 13635-900 , Brazil.
authors: Helena Lage
authors: HL
authors: Ferreira
authors: Fabienne
authors: F
authors: Rauw
authors: Jean François
authors: JF
authors: Pirlot
authors: Frédéric
authors: F
authors: Reynard
authors: Thierry
authors: T
authors: van den Berg
authors: Michel
authors: M
authors: Bublot
authors: Bénédicte
authors: B
authors: Lambrecht
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002645:Chickens; D005822:Genetic Vectors / Q000235:genetics; D056724:Immunity, Humoral / Q000276:immunology*; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology* / Q000472:pathogenicity; D005585:Influenza in Birds / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000235:genetics; D011201:Poultry Diseases / Q000517:prevention & control*; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000627:therapeutic use; D017201:Virus Shedding
chemical_list: D014765:Viral Vaccines