title: Opposite action of R2R3-MYBs from different subgroups on key genes of the shikimate and monolignol pathways in spruce.
abstract: Redundancy and competition between R2R3-MYB activators and repressors on common target genes has been proposed as a fine-tuning mechanism for the regulation of plant secondary metabolism. This hypothesis was tested in white spruce [Picea glauca (Moench) Voss] by investigating the effects of R2R3-MYBs from different subgroups on common targets from distinct metabolic pathways. Comparative analysis of transcript profiling data in spruces overexpressing R2R3-MYBs from loblolly pine (Pinus taeda L.), PtMYB1, PtMYB8, and PtMYB14, defined a set of common genes that display opposite regulation effects. The relationship between the closest MYB homologues and 33 putative target genes was explored by quantitative PCR expression profiling in wild-type P. glauca plants during the diurnal cycle. Significant Spearman's correlation estimates were consistent with the proposed opposite effect of different R2R3-MYBs on several putative target genes in a time-related and tissue-preferential manner. Expression of sequences coding for 4CL, DHS2, COMT1, SHM4, and a lipase thio/esterase positively correlated with that of PgMYB1 and PgMYB8, but negatively with that of PgMYB14 and PgMYB15. Complementary electrophoretic mobility shift assay (EMSA) and transactivation assay provided experimental evidence that these different R2R3-MYBs are able to bind similar AC cis-elements in the promoter region of Pg4CL and PgDHS2 genes but have opposite effects on their expression. Competitive binding EMSA experiments showed that PgMYB8 competes more strongly than PgMYB15 for the AC-I MYB binding site in the Pg4CL promoter. Together, the results bring a new perspective to the action of R2R3-MYB proteins in the regulation of distinct but interconnecting metabolism pathways.
authors: Center for Forest Research, Université Laval, Québec, QC G1V A06, Canada.
authors: Claude
authors: C
authors: Bomal
authors: Isabelle
authors: I
authors: Duval
authors: Isabelle
authors: I
authors: Giguère
authors: Élise
authors: É
authors: Fortin
authors: Sébastien
authors: S
authors: Caron
authors: Don
authors: D
authors: Stewart
authors: Brian
authors: B
authors: Boyle
authors: Armand
authors: A
authors: Séguin
authors: John J
authors: JJ
authors: MacKay
mesh_terms: D001483:Base Sequence; D053898:Biosynthetic Pathways / Q000235:genetics; D018744:DNA, Plant / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D008031:Lignin / Q000378:metabolism*; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D028222:Picea / Q000235:genetics* / Q000378:metabolism*; D028223:Pinus / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012765:Shikimic Acid / Q000378:metabolism*; D018709:Statistics, Nonparametric; D013997:Time Factors; D015533:Transcriptional Activation / Q000235:genetics
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; D012333:RNA, Messenger; D012765:Shikimic Acid; D008031:Lignin
keywords: Conifers; R2R3-MYB evolution; phenylpropanoid pathway; protein–DNA binding; transcriptional network.