title: Determination of acetamiprid and IM-1-2 in postmortem human blood, liver, stomach contents by HPLC-DAD.
abstract: An HPLC-DAD method was developed to detect and quantify a neonicotinoid insecticide acetamiprid (ATP) and its metabolite IM-1-2 in autopsy samples of a fatal intoxication case. The postmortem blood and tissue distribution of ATP and IM-1-2 was determined for the first time. The method showed acceptable precisions and recoveries with relative standard deviations of <10% for ATP level and 1.38 % for IM-1-2. The detection and quantification limits for ATP were 0.015 μg/mL and 0.030 μg/mL for blood and were 0.035 μg/g and 0.050 μg/g for liver samples, respectively. The mean contents of ATP were 0.79 μg/g in the liver, 47.35 μg/g in the stomach contents and 2.7 μg/mL in the blood. IM-1-2 content was 17.0 μg/g in the stomach contents. ATP and IM-1-2 were not detected in the urine. The presence of ATP and IM-1-2 in the samples was confirmed by GC-MS. The method can be exploited in future forensic casework.
authors: Department of Chemistry, Ministry of Justice, Forensic Medicine Council, Yenibosna, Bahçelievler, Istanbul, 34196, Turkey.
authors: Oya
authors: O
authors: Yeter
authors: Adnan
authors: A
authors: Aydın
mesh_terms: D000328:Adult; D002648:Child; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D053593:Forensic Toxicology; D008401:Gas Chromatography-Mass Spectrometry; D005766:Gastrointestinal Contents / Q000737:chemistry*; D006801:Humans; D007211:Indoles; D007306:Insecticides / Q000032:analysis* / Q000737:chemistry / Q000506:poisoning; D057230:Limit of Detection; D008099:Liver / Q000737:chemistry*; D008297:Male; D008301:Maleimides; D015394:Molecular Structure; D000073943:Neonicotinoids; D009570:Nitriles / Q000032:analysis* / Q000737:chemistry; D011180:Postmortem Changes; D011725:Pyridines / Q000032:analysis* / Q000737:chemistry / Q000506:poisoning
chemical_list: C554531:3-(4-fluorophenylethylamino)-1-methyl-4-(2-methyl-1H-indol-3-yl)-1H-pyrrole-2,5-dione; D007211:Indoles; D007306:Insecticides; D008301:Maleimides; D000073943:Neonicotinoids; D009570:Nitriles; D011725:Pyridines; C464485:acetamiprid
keywords: IM-1-2; acetamiprid; forensic science; intoxication; liver; postmortem blood; stomach contents; urine