title: Protective effect of 2-aminoethyl diphenylborinate on acute ischemia-reperfusion injury in the rat kidney.
abstract: BACKGROUND
  To investigate the protective effect of 2-aminoethyl diphenylborinate (2-APB) against ischemia-reperfusion (I/R) injury in the rat kidney by an experimental study.
  
  
  METHODS
  Thirty male Sprague-Dawley rats were randomly divided into the following three groups: (1) sham group, (2) I/R group, and (3) I/R + 2-APB group. Renal I/R injury was induced by clamping the left renal pedicle for 45 min after right nephrectomy, followed by 3 h of reperfusion. The therapeutic agent 2-APB was administered intravenously at a dose of 2 mg/kg 10 min before renal ischemia. Glutathione, superoxide dismutase, total antioxidant capacity, malondialdehyde, tumor necrosis factor α, interleukin 6, aspartate aminotransferase, alanine aminotransferase, and creatinine levels were measured from blood samples, and the rats were sacrificed subsequently. Tissue samples were scored histopathologically. Visualization of apoptotic cells was performed using the terminal deoxynucleotidyl transferase dUTP nick end labeling staining method.
  
  
  RESULTS
  2-APB significantly reduced serum malondialdehyde, tumor necrosis factor α, interleukin 6, aspartate aminotransferase, alanine aminotransferase, and creatinine levels in the I/R injury group. However, glutathione, superoxide dismutase, and total antioxidant capacity levels increased significantly. Histopathologic scores were significantly better and the rate of apoptosis was lower in the 2-APB group.
  
  
  CONCLUSIONS
  2-APB reduces oxidative stress and damage caused by renal I/R injury. The results of this study demonstrate that 2-APB can be used as an effective agent against I/R injury in the kidney.
authors: Department of General Surgery, Balıkesir University Faculty of Medicine, Balıkesir, Turkey. Electronic address: muratyildar@hotmail.com.
authors: Murat
authors: M
authors: Yildar
authors: Department of Biochemistry, Balıkesir University Faculty of Veterinary Medicine, Balıkesir, Turkey.
authors: Hasan
authors: H
authors: Aksit
authors: Department of Pharmacology, Medical Pharmacology and Toxicology, Balıkesir University Faculty of Medicine, Balıkesir, Turkey.
authors: Oguzhan
authors: O
authors: Korkut
authors: Department of Pathology, Uludağ University Faculty of Veterinary Medicine, Bursa, Turkey.
authors: Musa O
authors: MO
authors: Ozyigit
authors: Department of Histology, Balıkesir University Faculty of Medicine, Balıkesir, Turkey.
authors: Bahar
authors: B
authors: Sunay
authors: Department of Medical Biochemistry, Balıkesir University Faculty of Medicine, Balıkesir, Turkey.
authors: Kamil
authors: K
authors: Seyrek
mesh_terms: D058186:Acute Kidney Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D001896:Boron Compounds / Q000494:pharmacology*; D002120:Calcium Channel Agonists / Q000494:pharmacology*; D003404:Creatinine / Q000378:metabolism; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: C538758:2-aminoethyl diphenylborinate; D000975:Antioxidants; D001896:Boron Compounds; D002120:Calcium Channel Agonists; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D003404:Creatinine; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: 2-APB; Antioxidants; Calcium channels; Kidney failure; Oxidative stress