title: Chitosan dermal substitute and chitosan skin substitute contribute to accelerated full-thickness wound healing in irradiated rats.
abstract: Wounds with full-thickness skin loss are commonly managed by skin grafting. In the absence of a graft, reepithelialization is imperfect and leads to increased scar formation. Biomaterials can alter wound healing so that it produces more regenerative tissue and fewer scars. This current study use the new chitosan based biomaterial in full-thickness wound with impaired healing on rat model. Wounds were evaluated after being treated with a chitosan dermal substitute, a chitosan skin substitute, or duoderm CGF. Wounds treated with the chitosan skin substitute showed the most re-epithelialization (33.2 ± 2.8%), longest epithelial tongue (1.62 ± 0.13 mm), and shortest migratory tongue distance (7.11 ± 0.25 mm). The scar size of wounds treated with the chitosan dermal substitute (0.13 ± 0.02 cm) and chitosan skin substitute (0.16 ± 0.05 cm) were significantly decreased (P < 0.05) compared with duoderm (0.45 ± 0.11 cm). Human leukocyte antigen (HLA) expression on days 7, 14, and 21 revealed the presence of human hair follicle stem cells and fibroblasts that were incorporated into and surviving in the irradiated wound. We have proven that a chitosan dermal substitute and chitosan skin substitute are suitable for wound healing in full-thickness wounds that are impaired due to radiation.
authors: Reconstructive Sciences Unit, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kelantan, Malaysia.
authors: Abu Bakar
authors: AB
authors: Mohd Hilmi
authors: Ahmad Sukari
authors: AS
authors: Halim
authors: Hasnan
authors: H
authors: Jaafar
authors: Abu Bakar
authors: AB
authors: Asiah
authors: Asma
authors: A
authors: Hassan
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000627:therapeutic use*; D048271:Chitosan / Q000737:chemistry / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D051381:Rats; D016038:Skin Transplantation; D017768:Skin, Artificial; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D048271:Chitosan