title: Study on optimisation of extraction process of tanshinone IIA and its mechanism of induction of gastric cancer SGC7901 cell apoptosis.
abstract: The objective of this paper was to investigate the extraction process of tanshinone IIA and its mechanism of induction of gastric cancer SGC7901 cell apoptosis. Extraction process of tanshinone IIA was optimised by orthogonal experimental method, and its effect on gastric cancer SGC7901 cell apoptosis was observed using MTT assay and electron microscopy. The optimum extraction process of tanshinone IIA was as follows: addition of a 10-fold amount of 80% ethanol, one-time extraction, and extraction time of 45 minutes. The study concluded that tanshinone IIA can induce apoptosis of gastric cancer SGC7901 cells.
authors: Department of Hepatic Surgery, National Hepatobiliary and Enteric Surgery Research Center, Ministry of Health, Central South University 410008, Hunan Provence, China.
authors: Jianhong
authors: J
authors: Hou
authors: Jiantai
authors: J
authors: He
authors: Xin
authors: X
authors: Jin
authors: Tian
authors: T
authors: Hu
authors: Yangde
authors: Y
authors: Zhang
mesh_terms: D045784:Abietanes / Q000494:pharmacology*; D000230:Adenocarcinoma; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D046529:Microscopy, Electron, Transmission; D010936:Plant Extracts / Q000494:pharmacology*; D027561:Salvia miltiorrhiza; D013274:Stomach Neoplasms
chemical_list: D045784:Abietanes; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; C021751:tanshinone
keywords: MTT assay; Tanshinone IIA; gastric cancer SGC7901 cells