title: NADH changes during hypoxia, ischemia, and increased work differ between isolated heart preparations.
abstract: Langendorff-perfused hearts and working hearts are established isolated heart preparation techniques that are advantageous for studying cardiac physiology and function, especially when fluorescence imaging is a key component. However, oxygen and energy requirements vary widely between isolated heart preparations. When energy supply and demand are not in harmony, such as when oxygen is not adequately available, the imbalance is reflected in NADH fluctuations. As such, NADH imaging can provide insight into the metabolic state of tissue. Hearts from New Zealand white rabbits were prepared as mechanically silenced Langendorff-perfused hearts, Langendorff-perfused hearts, or biventricular working hearts and subjected to sudden changes in workload, instantaneous global ischemia, and gradual hypoxia while heart rate, aortic pressure, and epicardial NADH fluorescence were monitored. Fast pacing resulted in a dip in NADH upon initiation and a spike in NADH when pacing was terminated in biventricular working hearts only, with the magnitude of the changes greatest at the fastest pacing rate. Working hearts were also most susceptible to changes in oxygen supply; NADH was at half-maximum value when perfusate oxygen was at 67.8 ± 13.7%. Langendorff-perfused and mechanically arrested hearts were the least affected by low oxygen supply, with half-maximum NADH occurring at 42.5 ± 5.0% and 23.7 ± 4.6% perfusate oxygen, respectively. Although the biventricular working heart preparation can provide a useful representation of mechanical in vivo heart function, it is not without limitations. Understanding the limitations of isolated heart preparations is crucial when studying cardiac function in the context of energy supply and demand.
authors: Department of Electrical and Computer Engineering, The George Washington University, Washington, District of Columbia; and.
authors: Anastasia M
authors: AM
authors: Wengrowski
authors: Sarah
authors: S
authors: Kuzmiak-Glancy
authors: Rafael
authors: R
authors: Jaimes
authors: Matthew W
authors: MW
authors: Kay
mesh_terms: D000818:Animals; D006321:Heart / Q000503:physiopathology*; D006339:Heart Rate / Q000502:physiology; D000860:Hypoxia / Q000201:enzymology* / Q000503:physiopathology; D017202:Myocardial Ischemia / Q000201:enzymology* / Q000503:physiopathology; D009206:Myocardium / Q000201:enzymology*; D009243:NAD / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D011817:Rabbits
chemical_list: D009243:NAD; D010100:Oxygen
keywords: Langendorff; biventricular working; blebbistatin; nadh imaging; workload