title: Silencing of DLGAP5 by siRNA significantly inhibits the proliferation and invasion of hepatocellular carcinoma cells.
abstract: BACKGROUND
  The dysregulation of oncogenes and tumor suppressor genes plays an important role in many cancers, including hepatocellular carcinoma (HCC), which is one of the most common cancers in the world. In a previous microarray experiment, we found that DLGAP5 is overexpressed in HCCs. However, whether the up-regulation of DLGAP5 contributes to hepatocarcinogenesis remains unclear.
  
  
  RESULTS
  In this study, we showed that DLGAP5 was significantly up-regulated in 76.4% (168 of 220) of the analyzed HCC specimens when compared with adjacent liver tissue. DLGAP5 overexpression was evident in 25% (22 of 88) of the HCC specimens without AFP expression, suggesting that DLGAP5 may be a novel biomarker for HCC pathogenesis. The silencing of DLGAP5 gene expression by RNA interference significantly suppressed cell growth, migration and colony formation in vitro. The expression level of DLGAP5 was also found to be related to the methylation level of its promoter in the HCC specimens.
  
  
  CONCLUSIONS
  Taken together, these data suggest that the expression of DLGAP5 is regulated by methylation and that the up-regulation of DLGAP5 contributes to HCC tumorigenesis by promoting cell proliferation.
authors: Institute of Hepatobiliary Surgery, Hospital Affiliated of Guilin Medical University, Guangxi Key Laboratory of Molecular Medicine, People's Republic of China in liver injury and repair, Hepatology Institute of Guilin Medical University, Guilin, Guangxi Zhuang Autonomous Region, China.
authors: Weijia
authors: W
authors: Liao
authors: Weilong
authors: W
authors: Liu
authors: Qing
authors: Q
authors: Yuan
authors: Xing
authors: X
authors: Liu
authors: Ying
authors: Y
authors: Ou
authors: Songqing
authors: S
authors: He
authors: Shengguang
authors: S
authors: Yuan
authors: Liling
authors: L
authors: Qin
authors: Qian
authors: Q
authors: Chen
authors: Kate
authors: K
authors: Nong
authors: Minghui
authors: M
authors: Mei
authors: Jian
authors: J
authors: Huang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D019175:DNA Methylation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism
chemical_list: C465041:DLGAP5 protein, human; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering