title: Thawed human sperm quality is influenced by the volume of the cryopreserved specimen.
abstract: OBJECTIVE
  To test the effect of sperm specimen volume in the freezing-thawing process on specimen quality.
  
  
  METHODS
  Experimental prospective study.
  
  
  METHODS
  Tertiary academic medical center.
  
  
  METHODS
  Fifty high-quality sperm donors donated ∼3 times each. Sperm samples were split into two aliquots and frozen in volumes of 0.25 mL and 0.5 mL.
  
  
  METHODS
  Semen analyses.
  
  
  METHODS
  Eight sperm quality parameters of thawed specimens.
  
  
  RESULTS
  Thawed 0.5-mL specimens had a higher percentage of motility and viability, progressive motility concentration, percentage of cells with high mitochondrial membrane potential, and intact chromatin compared with 0.25-mL specimens. Although there were fewer cells with intact acrosomes in the 0.5-mL thawed samples, they had a similar ability to respond to ionophore by acrosome reaction as the 0.25-mL specimens. Both groups had similar percentages of cells with oxidative stress and numbers of cells that bound to the zona pellucida. The remaining air volume in the straw and freezing medium composition had a minimal effect on tested parameters.
  
  
  CONCLUSIONS
  Better quality thawed human sperm was achieved after cryopreservation of high volumes compared with low volumes of specimens. Air volume in the straw had no influence on specimen quality.
authors: Institute for the Study of Fertility, Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors: Ayelet
authors: A
authors: Abush
authors: Institute for the Study of Fertility, Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors: Ron
authors: R
authors: Hauser
authors: Institute for the Study of Fertility, Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors: Gedalia
authors: G
authors: Paz
authors: Institute for the Study of Fertility, Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors: Sandra E
authors: SE
authors: Kleiman
authors: Institute for the Study of Fertility, Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors: Ofer
authors: O
authors: Lehavi
authors: Institute for the Study of Fertility, Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors: Haim
authors: H
authors: Yavetz
authors: Institute for the Study of Fertility, Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel. Electronic address: layogev@zahav.net.il.
authors: Leah
authors: L
authors: Yogev
mesh_terms: D000177:Acrosome / Q000502:physiology; D048429:Cell Size; D015925:Cryopreservation / Q000379:methods*; D006801:Humans; D008297:Male; D011446:Prospective Studies; D011897:Random Allocation; D012662:Semen Preservation / Q000379:methods*; D013076:Sperm Count / Q000379:methods; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*
keywords: Acrosome integrity; frozen specimen volume; thawed sperm quality