title: [The association study of the LIPC -250g/A polymorphism and high-carbohydrate/low-fat diet induced serum lipid and apolipoprotein concentration changes in healthy youth].
abstract: OBJECTIVE
  To investigate the role of the - 250G/A polymorphism in the promoter region of hepatic lipase gene (LIPC) on serum lipid profile and its interactions with a high-carbohydrate/low-fat (HC/LF) diet on serum lipid profiles in a young healthy Chinese population.
  
  
  METHODS
  After a stabilization diet for seven days, fifty-six young healthy subjects (27 males, 29 females) were given the HC/LF diet for six days. The serum lipid profiles were analyzed of the twelve-hour fasting venous blood samples collected in the mornings of the first, the eighth and the fourteenth days. The concentrations of serum apolipoproteins were measured. The LIPC -250G/A polymorphism were analyzed.
  
  
  RESULTS
  At baseline, the female subjects with the GG genotype had significantly higher high-density lipoprotein cholesterol (HDL-C) (P= 0. 041) and apolipoprotein A- I (Apo A- I ) (P= 0. 020) than the male subjects with the same genotype. After the stabilization diet, the females had significantly higher HDL-C (GG genotype: P=0. 021, A carriers: P=0. 014) and Apo A-I (GG genotype: P= 0. 035, A carriers: P= 0. 006) than the males in all genotypes. After the HC/LF diet, the female A carriers had significantly higher total cholesterol (TC) (P= 0. 042) than the male A carriers, and the females had significantly higher Apo A- I than the males in all genotypes (GG genotype: P=0. 010, A carriers: P=0. 009). Compared with thosebefore the HC / LF diet , TC ( males with GG genotype : P = 0. 013 , male A carriers: P = 0. 000 ; females with GG genotype: P=0. 025, female A carriers: P=0. 048) and low-density lipoprotein cholesterol (LDL-C) (males with GG genotype: P = 0. 028, male A carriers: P = 0. 000; females with GG genotype: P= 0. 004, female A carriers: P=0. 001) significantly decreased after the diet in all the subjects. Triglycerides (TAG) (GG genotype: P=0. 006, A carriers: P= 0. 001) significantly increased in the females regardless of the genotype. However, only in the male A carriers, HDL-C (P= 0. 011) and Apo A- I (P= 0. 041) significantly increased after the diet.
  
  
  CONCLUSIONS
  The A allele at the LIPC -250G/A polymorphism is associated with the HC/LF diet induced HDL-C and Apo A-I concentration changes in the males.
authors: Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors: Min-Shan
authors: MS
authors: Hu
authors: Zheng-Ke
authors: ZK
authors: Li
authors: Ding-Zhi
authors: DZ
authors: Fang
mesh_terms: D016632:Apolipoprotein A-I / Q000097:blood*; D008076:Cholesterol, HDL / Q000097:blood*; D018752:Diet, Fat-Restricted; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D004041:Dietary Fats / Q000008:administration & dosage*; D005260:Female; D056726:Genetic Association Studies; D005838:Genotype; D006801:Humans; D008049:Lipase / Q000235:genetics*; D008055:Lipids / Q000097:blood; D008099:Liver / Q000378:metabolism; D008297:Male; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D055815:Young Adult
chemical_list: D016632:Apolipoprotein A-I; D008076:Cholesterol, HDL; D004040:Dietary Carbohydrates; D004041:Dietary Fats; C504173:LIPC protein, human; D008055:Lipids; D008049:Lipase