title: Myosin II regulates actin rearrangement-related structural synaptic plasticity during conditioned taste aversion memory extinction.
abstract: Similar to memory formation, memory extinction is also a new learning process that requires synaptic plasticity. Actin rearrangement is fundamental for synaptic plasticity, however, whether actin rearrangement in the infralimbic cortex (IL) plays a role in memory extinction, as well as the mechanisms underlying it, remains unclear. Here, using a conditioned taste aversion (CTA) paradigm, we demonstrated increased synaptic density and actin rearrangement in the IL during the extinction of CTA. Targeted infusion of an actin rearrangement inhibitor, cytochalasin D, into the IL impaired memory extinction and de novo synapse formation. Notably, we also found increased myosin II phosphorylation in the IL during the extinction of CTA. Microinfusion of a specific inhibitor of the myosin II ATPase, blebbistatin (Blebb), into the IL impaired memory extinction as well as the related actin rearrangement and changes in synaptic density. Moreover, the extinction deficit and the reduction of synaptic density induced by Blebb could be rescued by the actin polymerization stabilizer jasplakinolide (Jasp), suggesting that myosin II acts via actin filament polymerization to stabilize synaptic plasticity during the extinction of CTA. Taken together, we conclude that myosin II may regulate the plasticity of actin-related synaptic structure during memory extinction. Our studies provide a molecular mechanism for understanding the plasticity of actin rearrangement-associated synaptic structure during memory extinction.
authors: Department of Neurobiology, Shandong Provincial Key Laboratory of Mental Disorders, School of Medicine, Shandong University, No. 44 Wenhua Xi Road, Jinan, 250012, Shandong, People's Republic of China.
authors: Ai-Ling
authors: AL
authors: Bi
authors: Yue
authors: Y
authors: Wang
authors: Shuang
authors: S
authors: Zhang
authors: Bo-Qin
authors: BQ
authors: Li
authors: Zong-Peng
authors: ZP
authors: Sun
authors: Hong-Sheng
authors: HS
authors: Bi
authors: Zhe-Yu
authors: ZY
authors: Chen
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D001362:Avoidance Learning / Q000502:physiology*; D003214:Conditioning, Classical / Q000502:physiology; D005108:Extinction, Psychological / Q000502:physiology*; D008297:Male; D024462:Myosin Type II / Q000378:metabolism*; D009473:Neuronal Plasticity; D010766:Phosphorylation; D017397:Prefrontal Cortex / Q000502:physiology* / Q000648:ultrastructure; D017208:Rats, Wistar; D013569:Synapses / Q000378:metabolism / Q000648:ultrastructure; D013649:Taste
chemical_list: D000199:Actins; D024462:Myosin Type II