title: Effects of heat shock on survival, proliferation and differentiation of mouse neural stem cells.
abstract: Hyperthermia during pregnancy is a significant cause of reproductive problems ranging from abortion to congenital defects of the central nervous system (CNS), including neural tube defects and microcephaly. Neural stem cells (NSCs) can proliferate and differentiate into neurons and glia, playing a key role in the formation of the CNS. Here, we examined the effects of heat shock on homogeneous proliferating NSCs derived from mouse embryonic stem cells. After heat shock at 42 °C for 20 min, the proliferating NSCs continued to proliferate, although subtle changes were observed in gene expression and cell survival and proliferation. In contrast, heat shock at 43 °C caused a variety of responses: the up-regulation of genes encoding heat shock proteins (HSP), induction of apoptosis, temporal inhibition of cell proliferation and retardation of differentiation. Finally, effects of heat shock at 44 °C were severe, with almost all cells disappearing and the remaining cells losing the capacity to proliferate and differentiate. These temperature-dependent effects of heat shock on NSCs may be valuable in elucidating the mechanisms by which hyperthermia during pregnancy causes various reproductive problems.
authors: Laboratory of Regenerative Neurosciences, Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 7-2-10 Higashi-ogu, Arakawa-ku, Tokyo, 116-8551, Japan. Electronic address: omori-hiroyuki@ed.tmu.ac.jp.
authors: Hiroyuki
authors: H
authors: Omori
authors: Department of Chemistry, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo, 181-8611, Japan. Electronic address: otsu_masa@ks.kyorin-u.ac.jp.
authors: Masahiro
authors: M
authors: Otsu
authors: Laboratory of Regenerative Neurosciences, Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 7-2-10 Higashi-ogu, Arakawa-ku, Tokyo, 116-8551, Japan. Electronic address: t05bt021@gmail.com.
authors: Asami
authors: A
authors: Suzuki
authors: Department of Biochemistry, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama, 236-0004, Japan. Electronic address: nakayama@med.yokohama-cu.ac.jp.
authors: Takashi
authors: T
authors: Nakayama
authors: Department of Chemistry, Graduate School of Science, Chiba University, 1-33 Yayoi-Cho, Inage-ku, Chiba 263-8522, Japan; Center for General Education, Chiba University, Chiba, Japan. Electronic address: akama@faculty.chiba-u.jp.
authors: Kuniko
authors: K
authors: Akama
authors: Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 7-2-10 Higashi-ogu, Arakawa-ku, Tokyo, 116-8551, Japan. Electronic address: masaru@tmu.ac.jp.
authors: Masaru
authors: M
authors: Watanabe
authors: Laboratory of Regenerative Neurosciences, Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 7-2-10 Higashi-ogu, Arakawa-ku, Tokyo, 116-8551, Japan. Electronic address: juninoue@hs.tmu.ac.jp.
authors: Nobuo
authors: N
authors: Inoue
mesh_terms: D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D006360:Heat-Shock Proteins / Q000378:metabolism; D018869:Heat-Shock Response; D006358:Hot Temperature; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D006360:Heat-Shock Proteins
keywords: Apoptosis; Differentiation; Heat shock; Neural stem cells; Neural tube defects; Proliferation