title: Thermal stability and starch degradation profile of α-amylase from Streptomyces avermitilis.
abstract: Amylases from Streptomyces are useful in the production of maltooligosaccharides, but they have weak thermal stability at temperatures higher than 40 °C. In this study, α-amylase (SAV5981 gene of Streptomyces avermitilis) was expressed from Streptomyces lividans 1326 and purified by ammonium sulfate fractionation followed by anionic chromatography (Q-HP sepharose). The properties of the purified SAV5981 amylase were determined by the starch-iodine method. The effect of metal ions on amylase activity was investigated. The optimal temperature shifted from 25 to 50 °C with the addition of the Ca(2+) ion. The thermal stability of SAV5981 was also dramatically enhanced by the addition of 10 mM CaCl2. Improvement of the thermal stability of SAV5981 was examined by CD spectra in the presence and the absence of the Ca(2+) ion. Thin-layer chromatography (TLC) analysis and HPLC analysis of starch degradation revealed that SAV5981 mainly produced maltose and maltotriose, not glucose. The maltoorigosaccharide-producing amylase examined in this study has the potential in the industrial application of oligosaccharide production.
authors: Organization of Advanced Science and Technology, Kobe University.
authors: Sang Youn
authors: SY
authors: Hwang
authors: Kazunori
authors: K
authors: Nakashima
authors: Naoko
authors: N
authors: Okai
authors: Fumiyoshi
authors: F
authors: Okazaki
authors: Michiru
authors: M
authors: Miyake
authors: Koichi
authors: K
authors: Harazono
authors: Chiaki
authors: C
authors: Ogino
authors: Akihiko
authors: A
authors: Kondo
mesh_terms: D004795:Enzyme Stability; D008320:Maltose / Q000096:biosynthesis; D008670:Metals / Q000494:pharmacology; D011487:Protein Conformation; D013213:Starch / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity / Q000187:drug effects; D013696:Temperature; D000516:alpha-Amylases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D008670:Metals; D008320:Maltose; D013213:Starch; D000516:alpha-Amylases