title: A novel PCR-based genotyping scheme for clinical Klebsiella pneumoniae.
abstract: OBJECTIVE
  To establish a PCR-based genotyping method for clinical Klebsiella pneumoniae.
  
  
  METHODS
  The prevalence of six serotype markers, 41 large variably presented gene clusters, and seven additional virulence markers were screened by PCR in 327 clinical K. pneumoniae strains from China.
  
  
  RESULTS
  Detection of serotype markers enabled the identification of capsular serotypes K1, K2, K5, K20, K54 and K57. K. pneumoniae isolates of different origins gave distinct profiles of virulence loci, allowing us to gain a full overview of virulence gene distribution of the strains tested. A novel genotyping scheme was established to group clinical K. pneumoniae strains into distinct complexes based on the profiles of large variably presented gene clusters and virulence markers.
  
  
  CONCLUSIONS
  This PCR-based genotyping method would be useful to not only characterize genetic diversity and virulence gene distribution, but also for genotyping, origin tracing and risk estimation of K. pneumoniae.
authors: Nanlou Respiratory Diseases Department, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
authors: Zhenhong
authors: Z
authors: Chen
authors: Mengying
authors: M
authors: Liu
authors: Yujun
authors: Y
authors: Cui
authors: Li
authors: L
authors: Wang
authors: Yiquan
authors: Y
authors: Zhang
authors: Jingfu
authors: J
authors: Qiu
authors: Ruifu
authors: R
authors: Yang
authors: Changting
authors: C
authors: Liu
authors: Dongsheng
authors: D
authors: Zhou
mesh_terms: D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017720:Molecular Epidemiology / Q000379:methods; D058889:Molecular Typing / Q000379:methods*; D005810:Multigene Family; D016133:Polymerase Chain Reaction / Q000379:methods*; D037521:Virulence Factors / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D037521:Virulence Factors