title: Novel species including Mycobacterium fukienense sp. is found from tuberculosis patients in Fujian Province, China, using phylogenetic analysis of Mycobacterium chelonae/abscessus complex.
abstract: OBJECTIVE
  To identify the novel species 'Mycobacterium fukienense' sp. nov of Mycobacterium chelonae/abscessus complex from tuberculosis patients in Fujian Province, China.
  
  
  METHODS
  Five of 27 clinical Mycobacterium isolates (Cls) were previously identified as M. chelonae/abscessus complex by sequencing the hsp65, rpoB, 16S-23S rRNA internal transcribed spacer region (its), recA and sodA house-keeping genes commonly used to describe the molecular characteristics of Mycobacterium. Clinical Mycobacterium isolates were classified according to the gene sequence using a clustering analysis program. Sequence similarity within clusters and diversity between clusters were analyzed.
  
  
  RESULTS
  The 5 isolates were identified with distinct sequences exhibiting 99.8% homology in the hsp65 gene. However, a complete lack of homology was observed among the sequences of the rpoB, 16S-23S rRNA internal transcribed spacer region (its), sodA, and recA genes as compared with the M. abscessus. Furthermore, no match for rpoB, sodA, and recA genes was identified among the published sequences.
  
  
  CONCLUSIONS
  The novel species, Mycobacterium fukienense, is identified from tuberculosis patients in Fujian Province, China, which does not belong to any existing subspecies of M. chelonea/abscessus complex.
authors: State Key Laboratory for Infections Disease Prevention and Control/National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors: Yuan Yuan
authors: YY
authors: Zhang
authors: Yan Bing
authors: YB
authors: Li
authors: Ming Xiang
authors: MX
authors: Huang
authors: Xiu Qin
authors: XQ
authors: Zhao
authors: Li Shui
authors: LS
authors: Zhang
authors: Wen En
authors: WE
authors: Liu
authors: Kang Lin
authors: KL
authors: Wan
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009165:Mycobacterium Infections, Nontuberculous / Q000453:epidemiology / Q000382:microbiology*; D016926:Mycobacterium chelonae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D016415:Sequence Alignment; D014376:Tuberculosis / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial
keywords: Mycobacterium chelonae/abscessus complex; Mycobacterium fukienense; Species identification