title: [Apoptosis of A549 cells induced by cloned Noxa gene].
abstract: OBJECTIVE
  To clone the Noxa gene and to observe the apoptosis of A549 cells transfected with the recombinant plasmid of pcDNA-Noxa.
  
  
  METHODS
  The Noxa gene was obtained by PCR, and was cloned into pcDNA3. 1(-). A549 cells were transfected with the recombinant plasmid of pcDNA-Noxa. Western blot analysis was performed to determine the overexpression of Noxa. A549 cells were stained with Hoechst 33258 to observe the apoptosis.
  
  
  RESULTS
  The recombinant plasmid of pcDNA-Noxa was successfully constructed evidenced by endonuclease digestion and sequence analysis. The overexpression of Noxa was identified using Western blot analysis. The recombinant plasmid of pcDNA-Noxa induced apoptosis of A549 cells.
  
  
  CONCLUSIONS
  Nora has exhibited potential pro-apoptotic activity against A549 cells. This study is a foundation for further research into pro-apoptotic activity of Noxa gene.
authors: Chegdu Medical College, Chengdu 610083, China.
authors: Deng-bang
authors: DB
authors: Chen
authors: Kang
authors: K
authors: Cao
authors: Jing
authors: J
authors: Yang
authors: Qu
authors: Q
authors: Pan
authors: Tian
authors: T
authors: Chen
authors: Jin-Chuan
authors: JC
authors: Li
authors: Xin
authors: X
authors: Wang
authors: Jian-Hua
authors: JH
authors: Yin
authors: Yi
authors: Y
authors: Zou
authors: Chang-You
authors: CY
authors: Wei
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D017209:Apoptosis / Q000235:genetics*; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: C498702:PMAIP1 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2