title: Silicate fiber-based 3D cell culture system for anticancer drug screening.
abstract: BACKGROUND
  Three-dimensional (3D) in vitro cultures can recapitulate the physiological in vivo microenvironment. 3D Modeling techniques have been investigated and applied in anticancer drug screening.
  
  
  METHODS
  A silicate fiber scaffold was used for 3D cell cultures, and used to model the efficacy of anticancer drugs, such as mytomicin C and doxorubicin.
  
  
  RESULTS
  A unique 3D structure was observed in 13 human tumor cell lines on scaffold, and these cells exhibited higher drug resistance than cells in two-dimensional (2D) cultures. Furthermore, the production of lactate and expression of the nuclear factor-kappa B (NF-κB)-regulated genes B cell lymphoma-2 (BCL2), cyclooxygenase-2 (COX2), and vascular endothelial growth factor (VEGF) were higher in 3D cultures than in 2D cultures.
  
  
  CONCLUSIONS
  These findings suggest that a 3D model using a silicate fiber scaffold can mimic features of cancer, and is also a suitable model for the evaluation of anticancer drugs in vitro.
authors: Japan Bio Products Co., Ltd., Aikawa, Kurume, Fukuoka; Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Japan. t_morinaga@placenta-jbp.co.jp
authors: Yoshie
authors: Y
authors: Yamaguchi
authors: Dawei
authors: D
authors: Deng
authors: Yoshinori
authors: Y
authors: Sato
authors: Yung-Te
authors: YT
authors: Hou
authors: Rie
authors: R
authors: Watanabe
authors: Kohei
authors: K
authors: Sasaki
authors: Masaaki
authors: M
authors: Kawabe
authors: Eiichi
authors: E
authors: Hirano
authors: Tetsuo
authors: T
authors: Morinaga
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D001483:Base Sequence; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2 / Q000378:metabolism; D017931:DNA Primers; D004317:Doxorubicin / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005786:Gene Expression Regulation / Q000502:physiology; D006019:Glycolysis; D006801:Humans; D016685:Mitomycin / Q000494:pharmacology; D016328:NF-kappa B / Q000502:physiology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D017640:Silicates; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D017931:DNA Primers; D016328:NF-kappa B; D019253:Proto-Oncogene Proteins c-bcl-2; D017640:Silicates; D042461:Vascular Endothelial Growth Factor A; D016685:Mitomycin; D004317:Doxorubicin; D051546:Cyclooxygenase 2
keywords: Three-dimensional (3D) culture; anticancer drug screening; drug resistance; silicate fiber scaffold