title: Characterization of lysis of the multicellular Cyanobacterium Limnothrix/Pseudanabaena sp. strain ABRG5-3.
abstract: The cyanobacterium semi-filamentous multicellular strain ABRG5-3 undergoes cell lysis as a unique feature that occurs due to growth condition changes from normal cultivation with shaking to static cultivation without shaking in liquid culture (Nishizawa et al., 2010). Microscopic observation and energy dispersive X-ray spectrometer (EDX) analysis have revealed that lysis is involved in the accumulation of polyphosphate compounds and the disintegration of thylakoid membranes in cells. Static cultivation, dark or red light exposure, and temperature (22 to 42 °C) conditions were found to be effective factors for the induction of lysis. Moreover, stress induced by salts, osmotic pressure with sucrose, and the depletion of nitrogen or phosphate in cultures also induced ABRG5-3 cell lysis. Based on these results, we discuss lysis and its utilization in the biotechnology industry.
authors: College of Agriculture, Ibaraki University.
authors: Chifumi
authors: C
authors: Kitazaki
authors: Setsuko
authors: S
authors: Numano
authors: Akira
authors: A
authors: Takanezawa
authors: Tomoyasu
authors: T
authors: Nishizawa
authors: Makoto
authors: M
authors: Shirai
authors: Munehiko
authors: M
authors: Asayama
mesh_terms: D002245:Carbon Dioxide / Q000378:metabolism; D049109:Cell Proliferation; D000458:Cyanobacteria / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D009584:Nitrogen / Q000378:metabolism; D009997:Osmotic Pressure; D010710:Phosphates / Q000378:metabolism; D010788:Photosynthesis; D012492:Salts / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects
chemical_list: D010710:Phosphates; D012492:Salts; D002245:Carbon Dioxide; D009584:Nitrogen