title: Electrochemical patterning and detection of DNA arrays on a two-electrode platform.
abstract: We report a novel method of DNA array formation that is electrochemically formed and addressed with a two-electrode platform. Electrochemical activation of a copper catalyst, patterned with one electrode, enables precise placement of multiple sequences of DNA onto a second electrode surface. The two-electrode patterning and detection platform allows for both spatial resolution of the patterned DNA array and optimization of detection through DNA-mediated charge transport with electrocatalysis. This two-electrode platform has been used to form arrays that enable differentiation between well-matched and mismatched sequences, the detection of TATA-binding protein, and sequence-selective DNA hybridization.
authors: Division of Chemistry and Chemical Engineering, California Institute of Technology , Pasadena, California 91125, United States.
authors: Ariel
authors: A
authors: Furst
authors: Sally
authors: S
authors: Landefeld
authors: Michael G
authors: MG
authors: Hill
authors: Jacqueline K
authors: JK
authors: Barton
mesh_terms: D002384:Catalysis; D003300:Copper / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D006824:Hybridization, Genetic; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*
chemical_list: D003300:Copper; D004247:DNA