title: Plasma polymer-coated contact lenses for the culture and transfer of corneal epithelial cells in the treatment of limbal stem cell deficiency.
abstract: Extensive damage to the limbal region of the cornea leads to a severe form of corneal blindness termed as limbal stem cell deficiency (LSCD). Whereas most cases of corneal opacity can be treated with full thickness corneal transplants, LSCD requires stem cell transplantation for successful ocular surface reconstruction. Current treatments for LSCD using limbal stem cell transplantation involve the use of murine NIH 3T3 cells and human amniotic membranes as culture substrates, which pose the threat of transmission of animal-derived pathogens and donor tissue-derived cryptic infections. In this study, we aimed to produce surface modified therapeutic contact lenses for the culture and delivery of corneal epithelial cells for the treatment of LSCD. This approach avoids the possibility of suture-related complications and is completely synthetic. We used plasma polymerization to deposit acid functional groups onto the lenses at various concentrations. Each surface was tested for its suitability to promote corneal epithelial cell adhesion, proliferation, retention of stem cells, and differentiation and found that acid-based chemistries promoted better cell adhesion and proliferation. We also found that the lenses coated with a higher percentage of acid functional groups resulted in a higher number of cells transferred onto the corneal wound bed in rabbit models of LSCD. Immunohistochemistry of the recipient cornea confirmed the presence of autologous, transplanted 5-bromo-2'-deoxyuridine (BrdU)-labeled cells. Hematoxylin staining has also revealed the presence of a stratified epithelium at 26 days post-transplantation. This study provides the first evidence for in vivo transfer and survival of cells transplanted from a contact lens to the wounded corneal surface. It also proposes the possibility of using plasma polymer-coated contact lenses with high acid functional groups as substrates for the culture and transfer of limbal cells in the treatment of LSCD.
authors: 1 Surgical Research Unit, Centre for Eye Research Australia , Melbourne, Australia .
authors: Karl David
authors: KD
authors: Brown
authors: Suet
authors: S
authors: Low
authors: Indumathi
authors: I
authors: Mariappan
authors: Keren Maree
authors: KM
authors: Abberton
authors: Robert
authors: R
authors: Short
authors: Hong
authors: H
authors: Zhang
authors: Savitri
authors: S
authors: Maddileti
authors: Virender
authors: V
authors: Sangwan
authors: David
authors: D
authors: Steele
authors: Mark
authors: M
authors: Daniell
mesh_terms: D000179:Acrylates / Q000494:pharmacology; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003261:Contact Lenses; D003316:Corneal Diseases / Q000473:pathology / Q000628:therapy*; D004847:Epithelial Cells / Q000187:drug effects / Q000637:transplantation*; D019573:Epithelium, Corneal / Q000473:pathology*; D019793:Fluorescein / Q000378:metabolism; D006801:Humans; D016850:Limbus Corneae / Q000473:pathology*; D051379:Mice; D056951:Photoelectron Spectroscopy; D058626:Plasma Gases / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology*; D011817:Rabbits; D013234:Stem Cells / Q000187:drug effects / Q000473:pathology*; D013499:Surface Properties; D014182:Transplantation, Autologous
chemical_list: D000179:Acrylates; D058626:Plasma Gases; D011108:Polymers; C036658:acrylic acid; D019793:Fluorescein