title: LC-MS/MS in the routine clinical laboratory: has its time come?
abstract: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been increasingly used in routine clinical laboratories during the last two decades. The high specificity, sensitivity, and multi-analyte potential make it an ideal alternative to immunoassays or conventional high-performance liquid chromatography (HPLC). It also provides higher throughput than gas chromatography-mass spectrometry (GC-MS). LC-MS/MS also offers higher flexibility than immunoassays because LC-MS/MS assays are typically developed in-house. In addition, abundant information can be obtained from a single LC-MS/MS run which can produce a large amount of quantitative or qualitative data. In this review, typical LC-MS/MS clinical applications are presented, personal experiences are shared, and strengths and weakness are discussed. It is foreseeable that LC-MS/MS will become a key instrument in routine clinical laboratories.
authors: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Kowloon, HKSAR, China, s9362284@hkbu.edu.hk.
authors: Kelvin Sze-Yin
authors: KS
authors: Leung
authors: Bonnie Mei-Wah
authors: BM
authors: Fong
mesh_terms: D002851:Chromatography, High Pressure Liquid; D019411:Clinical Laboratory Techniques / Q000295:instrumentation / Q000379:methods*; D003955:Diagnostic Tests, Routine / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D053719:Tandem Mass Spectrometry