title: [Emdogain regulates the expression of bone sialoprotein gene in human dental pulp cells].
abstract: OBJECTIVE
  To analyze the effects of emdogain(EMD) on the expression of the bone sialoprotein(BSP) gene in human dental pulp cells and to elucidate the molecular mechanism of BSP gene regulated by EMD.
  
  
  METHODS
  Human dental pulp was harvested from premolars freshly extracted for orthodontic purpose and cultured. Cells were divided into different concentrations (25, 50, 100 and 250 mg/L) of EMD and control groups (Dulbecco's modified Eagle's medium). Total RNA of cells was extracted. Human BSP mRNA levels was detected with the real-time PCR. Regulations of EMD on human BSP protein levels were detected with Western blotting.
  
  
  RESULTS
  In the real-time PCR, at the same time point, there were significant differences on BSP mRNA levels between 25, 50, 100 and 250 mg/L EMD groups (7 d:1.79 ± 0.03, 2.03 ± 0.10, 2.67 ± 0.08, 2.94 ± 0.07) and control group (7 d:1.06 ± 0.11) (P < 0.001); at the different time point (1, 3, 5 and 7 d), the same dose(250 mg/L) of EMD stimulated human dental pulp cells, BSP mRNA (2.30 ± 0.06, 2.65 ± 0.05, 2.76 ± 0.05, 2.94 ± 0.07) was increased (P < 0.05). Treatment of human dental pulp cells with EMD (250 mg/L) increased the protein levels.
  
  
  CONCLUSIONS
  EMD increases BSP mRNA and protein levels in human dental pulp cells.
authors: Department of Cariology and Endodontology, Stomatological Hospital, Tianjin Medical University, Tianjin 300070, China.
authors: Zhen
authors: Z
authors: Chen
authors: Shuang
authors: S
authors: Wang
authors: Ying-hui
authors: YH
authors: Wang
authors: Ping
authors: P
authors: Gao
mesh_terms: D001641:Bicuspid; D002478:Cells, Cultured; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D058574:Integrin-Binding Sialoprotein / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D003746:Dental Enamel Proteins; D058574:Integrin-Binding Sialoprotein; D012333:RNA, Messenger; C109069:enamel matrix proteins