title: Phytochemical analysis, antibacterial, and antifungal assessment of aerial parts of Polygonatum verticillatum.
abstract: The current study was designed to assess the phytochemical profile, antibacterial, and antifungal activities of the crude methanol extract of the aerial parts of Polygonatum verticillatum (PA) and its various subsequent solvent fractions using agar well diffusion, agar tube dilution, and microdilution methods. Phytochemical analysis showed positive for different chemical groups and also contained marked quantity of saponin and flavonoid contents. Significant antibacterial activity was observed against various tested pathogenic bacteria. The only susceptible Gram-positive bacterium was Bacillus subtilis and their minimum inhibitory concentrations (MICs) measured ranged from 11-50 µg/ml. The sensitive Gram-negative bacteria were Salmonella typhi and Shigella flexeneri The estimated MICs were in the range of 2-7 µg/ml and 8-50 µg/ml for S. typhi and S. flexeneri, respectively. However, the antifungal activity of the plant was limited to Microsporum canis and their MICs ranged from 60 to 250 µg/ml. Our study confirmed significant antibacterial potential of the plant and substantiated its folk use in dysentery and pyrexia of multiple origins.
authors: Gandhara College of Pharmacy, Gandhara University, Peshawar, Pakistan Department of Pharmacy, Abdul Wali Khan University, Mardan, Pakistan hkdr2006@gmail.com.
authors: Haroon
authors: H
authors: Khan
authors: Department of Pharmacy, University of Peshawar, Peshawar, Pakistan.
authors: Muhammad
authors: M
authors: Saeed
authors: Department of Pharmacy, Abdul Wali Khan University, Mardan, Pakistan Department of Pharmacy, University of Peshawar, Peshawar, Pakistan.
authors: Naveed
authors: N
authors: Muhammad
authors: Gandhara College of Pharmacy, Gandhara University, Peshawar, Pakistan Department of Pharmacy, University of Peshawar, Peshawar, Pakistan.
authors: Samreen
authors: S
authors: Perviz
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology*; D000935:Antifungal Agents / Q000032:analysis / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008864:Microsporum / Q000187:drug effects; D064209:Phytochemicals / Q000032:analysis / Q000494:pharmacology*; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D031384:Polygonatum / Q000737:chemistry*; D012485:Salmonella typhi / Q000187:drug effects; D012503:Saponins / Q000032:analysis / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D005419:Flavonoids; D064209:Phytochemicals; D010936:Plant Extracts; D012503:Saponins
keywords: Polygonatum verticillatum (All); aerial parts; antibacterial; antifungal; total saponin and flavoniod contents