title: Organosolv pretreatment of rice straw for efficient acetone, butanol, and ethanol production.
abstract: Acetone-butanol-ethanol (ABE) was produced from rice straw using a process containing ethanol organosolv pretreatment, enzymatic hydrolysis, and fermentation by Clostridium acetobutylicum bacterium. Pretreatment of the straw with 75% (v/v) aqueous ethanol containing 1% w/w sulfuric acid at 150 °C for 60 min resulted in the highest total sugar concentration of 31 g/L in the enzymatic hydrolysis. However, the highest ABE concentration and productivity (10.5 g/L and 0.20 g/Lh, respectively) were obtained from the straw pretreated at 180 °C for 30 min. Enzymatic hydrolysis of the straw pretreated at 180 °C for 30 min with 5% solid loading resulted in glucose yield of 46.2%, which was then fermented to 80.3 g butanol, 21.1 g acetone, and 22.5 g ethanol, the highest overall yield of ABE production. Thus, the organosolv pretreatment can be applied for efficient production of the solvents from rice straw.
authors: Department of Chemical Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
authors: Hamid
authors: H
authors: Amiri
authors: Department of Chemical Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran; Industrial Biotechnology Group, Institute of Biotechnology and Bioengineering, Isfahan University of Technology, Isfahan 84156-83111, Iran. Electronic address: karimi@cc.iut.ac.ir.
authors: Keikhosro
authors: K
authors: Karimi
authors: Department of Chemical Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
authors: Hamid
authors: H
authors: Zilouei
mesh_terms: D020001:1-Butanol / Q000378:metabolism*; D000096:Acetone / Q000378:metabolism*; D001089:Arabinose / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D046969:Clostridium acetobutylicum / Q000187:drug effects / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis / Q000187:drug effects; D009930:Organic Chemicals / Q000494:pharmacology*; D012275:Oryza / Q000187:drug effects* / Q000378:metabolism; D012997:Solvents / Q000494:pharmacology*; D014866:Waste Products; D014994:Xylose / Q000378:metabolism
chemical_list: D009930:Organic Chemicals; D012997:Solvents; D014866:Waste Products; D000096:Acetone; D000431:Ethanol; D020001:1-Butanol; D014994:Xylose; D001089:Arabinose; D005947:Glucose
keywords: ABE fermentation; Lignocellulose; Organosolv; Pretreatment; Rice straw