title: [The study of interfering of endogenous VEGF-C genes and the protein expression gastric cancer cell with siRNA technique].
abstract: OBJECTIVE
  To explore the interfering effects of siRNA on endogenous VEGF-C genes and the protein expression in gastric cancer cells.
  
  
  METHODS
  Cultured gastric cancer cell line SGC7901 cells were prepared in vitro. Five siRNA primers of VEGF-C were designed depending on the sequence of Accession No. BC035212. 1 in Genbank. After homology analysis, the primers were synthesized and transfected into SGC7901 cells. The endogenous VEGF-C mRNA level and its protein expression were observed.
  
  
  RESULTS
  VEGF-C-siRNA was inserted into the gastric cancer cell successfully. Five siRNA primers of VEGF-C could inhibit VEGF-C genes and protein expression.
  
  
  CONCLUSIONS
  siRNA could block the endogenous VEGF-C genes and the protein expression in gastric cancer cell.
authors: Department of Gastroenterology, Nanshan Affiliated Hospital of Guangdong Medical College, Shenzhen 518000, China.
authors: Mei-Mei
authors: MM
authors: Guo
authors: Yong
authors: Y
authors: Wang
authors: Fang-Hai
authors: FH
authors: Han
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D014162:Transfection; D042582:Vascular Endothelial Growth Factor C / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D042582:Vascular Endothelial Growth Factor C