title: Cross-sectional and dynamic change of serum metabolite profiling for Hepatitis B-related acute-on-chronic liver failure by UPLC/MS.
abstract: Summary Acute-on-chronic liver failure(ACLF) is an increasingly recognized entity encompassing an acute deterioration of liver function and results in the failure of one or more organs with high short-term mortality. The focus of this study was to discover noninvasive and reliable biomarkers for the diagnosis and prognosis of hepatitis B-related ACLF. Ultra-performance liquid chromatography-mass spectrometry (UPLC/MS) was used to analyse serum metabolites of 28 patients with hepatitis B-related ACLF, 35 patients with Child-Pugh A cirrhosis, 30 patients with chronic hepatitis B and 35 healthy volunteers (HS). Characteristic metabolites were screened, identified and dynamically tracked to investigate their value for diagnosis and prognosis. After comparing serum metabolic profile of hepatitis B-related ACLF and Child-Pugh A cirrhosis, 99 characteristic metabolites were selected, and 38 of them were identified. Dynamic tracking model demonstrated that 17 metabolites were related to prognosis of hepatitis B-related ACLF, and there were also 11 metabolites which improved with treatment in the survival group. The correlations between these characteristic metabolites and the model for end-stage liver disease score were strong. These observations contributed to the investigation of the mechanisms of hepatitis B-related ACLF manifestation and progression on the metabolic level, and they provided information for the identification of biomarkers for the diagnosis and prognosis of hepatitis B-related ACLF.
authors: The Third Central Clinical College of Tianjin Medical University, Tianjin, China; Department of Hepatology, Tianjin Third Central Hospital, Tianjin, China.
authors: C Y
authors: CY
authors: Nie
authors: T
authors: T
authors: Han
authors: L
authors: L
authors: Zhang
authors: Y
authors: Y
authors: Li
authors: H
authors: H
authors: Liu
authors: S X
authors: SX
authors: Xiao
authors: Y
authors: Y
authors: Li
authors: H
authors: H
authors: Kang
authors: S Y
authors: SY
authors: Liu
mesh_terms: D000328:Adult; D000368:Aged; D001774:Blood Chemical Analysis; D002853:Chromatography, Liquid; D058625:End Stage Liver Disease / Q000175:diagnosis* / Q000473:pathology*; D005260:Female; D006509:Hepatitis B / Q000175:diagnosis* / Q000473:pathology*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D011379:Prognosis; D044967:Serum / Q000737:chemistry*
keywords: biomarkers; hepatitis B; liver failure; metabolomics; prognosis; serum