title: First identification of coexistence of blaNDM-1 and blaCMY-42 among Escherichia coli ST167 clinical isolates.
abstract: BACKGROUND
  Emergence of multidrug resistance in Enterobacteriaceae limits the selection of antimicrobials for treatment of infectious diseases. Identification of NDM-1 makes more difficulty in treating multidrug-resistant Enterobacteriaceae infections. Carbapenem-resistant Escherichia coli clinical isolates from a tertiary hospital in Wenzhou, east China, were investigated for NDM-1 production.
  
  
  RESULTS
  The two tested isolates were negative for modified Hodge test, but positive for a double-disc synergy test used for detecting metallo-β-lactamase production. E. coli WZ33 and WZ51 exhibited discrepant-level resistance to most clinically frequent used antimicrobials, but still susceptible to trimethoprim/sulfamethoxazole, amikacin, fosfomycin, tigecycline and polymyxin B. E. coli WZ33 and WZ51 were positive for bla(NDM-1) determined by PCR and DNA sequencing. Other than bla(NDM-1), E. coli WZ33 also harbored bla(CTX-M-14) and bla(CMY-42), while E. coli WZ51 simultaneously harbored blaSHV-12,bla(CTX-M-14) and bla(CMY-42). Carbapenem resistance for E. coli WZ51 and WZ33 could not be transferred to E. coli recipients through conjugation, but could be transferred to E. coli recipients by chemical transformation. The EcoR1-digested DNA pattern of plasmids from the transformant of E. coli WZ51 was different from that of E. coli WZ51. MLST showed that E. coli WZ33 and WZ51 belonged to an animal-associated clone (ST167).
  
  
  CONCLUSIONS
  The present study is the first report of bla(NDM-1) carriage in E. coli ST167 isolates and coexistence of bla(NDM-1) and bla(CMY-42) in same isolate. Systemic surveillance should focus on the dissemination of bla(NDM-1) among Enterobacteriaceae, especially E. coli ST167 clone associated with animal infection.
authors: Xueqing
authors: X
authors: Zhang
authors: Danping
authors: D
authors: Lou
authors: Yuanyuan
authors: Y
authors: Xu
authors: Yongpeng
authors: Y
authors: Shang
authors: Dan
authors: D
authors: Li
authors: Xiaoying
authors: X
authors: Huang
authors: Yuping
authors: Y
authors: Li
authors: Longhua
authors: L
authors: Hu
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, 2 Fuxue lane, Wenzhou 325000, China. 38805@163.com.
authors: Liangxing
authors: L
authors: Wang
authors: Fangyou
authors: F
authors: Yu
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002681:China; D004926:Escherichia coli / Q000201:enzymology* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D001618:beta-Lactamases; C561294:beta-lactamase CMY-42, E coli; C552864:beta-lactamase NDM-1