title: Case of cytomegalovirus-associated direct anti-globulin test-negative autoimmune hemolytic anemia.
abstract: A 1-year-old boy developed autoimmune hemolytic anemia after a negative direct anti-globulin test. The concentration of erythrocyte membrane-associated immunoglobulin G, determined using an immunoradiometric assay, correlated with disease activity. He was positive for cytomegalovirus (CMV) both serologically and by quantitative real-time polymerase chain reaction, indicating that his autoimmune hemolytic anemia was directly caused by CMV infection. Since anti-CMV immunoglobulin G was not absorbed by the patient's erythrocytes, cross-reaction between erythrocyte antigens and CMV was not likely a causative factor for hemolysis.
authors: Department of Pediatrics, Tokyo Dental College Ichikawa General Hospital, Chiba, Japan.
authors: Saeko
authors: S
authors: Kaneko
authors: Masanori
authors: M
authors: Sato
authors: Goro
authors: G
authors: Sasaki
authors: Hiroyuki
authors: H
authors: Eguchi
authors: Tsutomu
authors: T
authors: Oishi
authors: Toyomi
authors: T
authors: Kamesaki
authors: Hiroyuki
authors: H
authors: Kawaguchi
mesh_terms: D000744:Anemia, Hemolytic, Autoimmune / Q000097:blood* / Q000175:diagnosis / Q000821:virology*; D003298:Coombs Test; D003586:Cytomegalovirus Infections / Q000150:complications*; D005188:False Negative Reactions; D006801:Humans; D007223:Infant; D008297:Male
keywords: autoimmune hemolytic anemia; cytomegalovirus; direct anti-globulin test; immunoradiometric assay; real-time/quantitative polymerase chain reaction