title: Relapse-associated microRNA in gastric cancer patients after S-1 adjuvant chemotherapy.
abstract: S-1 has been recommended as adjuvant chemotherapy in patients after curative surgery for gastric cancer. However, some patients suffer recurrence even after S-1 adjuvant chemotherapy. The present study was conducted to find a predictive marker of the efficacy of S-1 adjuvant chemotherapy. We examined the microRNA (miRNA) expression of 35 patients who underwent S-1 adjuvant chemotherapy after curative surgery (R0) for pathological stage II or III gastric cancer. miRNAs were extracted from formalin-fixed, paraffin-embedded specimens for analysis and miRNA expression was examined using miRNA oligo chips. Fifteen patients relapsed and 20 did not over 5 years. Five miRNAs (miR-92b, 422a, 4732-5p, 4758-3p and 221) were highly expressed according to the tumor/normal (T/N) ratio in the patients who relapsed but not in those who did not relapse (P-value <0.05) by microarray analysis. If tumors showed high expression of 4 miRNAs (miR-92b, 422a, 4732-5p and 4758-3p) their positive predictive value of relapse was 93.8% and negative predictive value was 92.3%. In this case, their disease-free survival rate and overall survival rate were very poor. Our findings indicate that miR-92b, miR‑422a, miR-4732-5p and miR-4758-3p are closely associated with relapse following S-1 adjuvant chemotherapy in gastric cancer.
authors: Department of Surgery and Science, University of Toyama, Toyama 930‑0194, Japan.
authors: Tetsuya
authors: T
authors: Omura
authors: Department of Surgery and Science, University of Toyama, Toyama 930‑0194, Japan.
authors: Yutaka
authors: Y
authors: Shimada
authors: Department of Surgery and Science, University of Toyama, Toyama 930‑0194, Japan.
authors: Takuya
authors: T
authors: Nagata
authors: Department of Surgery and Science, University of Toyama, Toyama 930‑0194, Japan.
authors: Tomoyuki
authors: T
authors: Okumura
authors: Department of Surgical Pathology, University of Toyama, Toyama 930‑0194, Japan.
authors: Junya
authors: J
authors: Fukuoka
authors: Department of Surgery, Itoigawa General Hospital, Itoigawa, Niigata 941-8502, Japan.
authors: Fuminori
authors: F
authors: Yamagishi
authors: Department of Surgery, Saiseikai Toyama Hospital, Toyama 931-8533, Japan.
authors: Sadakatsu
authors: S
authors: Tajika
authors: Department of Surgery, Murakami Memorial Hospital, Asahi University, Gifu 500-8523, Japan.
authors: Sanae
authors: S
authors: Nakajima
authors: Department of Surgery, Murakami Memorial Hospital, Asahi University, Gifu 500-8523, Japan.
authors: Atsushi
authors: A
authors: Kawabe
authors: Department of Surgery and Science, University of Toyama, Toyama 930‑0194, Japan.
authors: Kazuhiro
authors: K
authors: Tsukada
mesh_terms: D000368:Aged; D000964:Antimetabolites, Antineoplastic / Q000627:therapeutic use*; D017024:Chemotherapy, Adjuvant; D018572:Disease-Free Survival; D004338:Drug Combinations; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000188:drug therapy / Q000235:genetics* / Q000401:mortality; D010094:Oxonic Acid / Q000627:therapeutic use*; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000401:mortality; D013534:Survival; D005641:Tegafur / Q000627:therapeutic use*
chemical_list: D000964:Antimetabolites, Antineoplastic; D004338:Drug Combinations; D035683:MicroRNAs; C079198:S 1 (combination); D005641:Tegafur; D010094:Oxonic Acid