title: Role of CD4(+) and CD8α(+) T cells in protective immunity against Edwardsiella tarda infection of ginbuna crucian carp, Carassius auratus langsdorfii.
abstract: Edwardsiella tarda is an intracellular pathogen that causes edwardsiellosis in fish. Our previous study suggests that cell-mediated immunity (CMI) plays an essential role in protection against E. tarda infection. In the present study, we adoptively transferred T-cell subsets sensitized with E. tarda to isogenic naïve ginbuna crucian carp to determination the T-cell subsets involved in protecting fish from E. tarda infection. Recipients of CD4(+) and CD8α(+) cells acquired significant resistance to infection with E. tarda 8 days after sensitization, indicating that helper T cells and cytotoxic T lymphocytes plays crucial roles in protective immunity to E. tarda. Moreover, transfer of sensitized CD8α(+) cells up-regulated the expression of genes encoding interferon-γ (IFN-γ) and perforin, suggesting that protective immunity to E. tarda involves cell-mediated cytotoxicity and interferon-γ-mediated induction of CMI. The results establish that CMI plays a crucial role in immunity against E. tarda. These findings provide novel insights into understanding the role of CMI to intracellular pathogens of fish.
authors: The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-8580, Japan.
authors: Masatoshi
authors: M
authors: Yamasaki
authors: Faculty of Fisheries, Kagoshima University, 4-50-20 Shimoarata, Kagoshima 890-0056, Japan. Electronic address: araki@fish.kagoshima-u.ac.jp.
authors: Kyosuke
authors: K
authors: Araki
authors: College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors: Teruyuki
authors: T
authors: Nakanishi
authors: National Research Institute of Aquaculture, Fisheries Research Agency, Minami-ise, Mie 516-0193, Japan.
authors: Chihaya
authors: C
authors: Nakayasu
authors: Faculty of Fisheries, Kagoshima University, 4-50-20 Shimoarata, Kagoshima 890-0056, Japan.
authors: Atsushi
authors: A
authors: Yamamoto
mesh_terms: D019264:Adoptive Transfer / Q000662:veterinary; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000382:microbiology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000382:microbiology*; D002347:Carps; D020609:Edwardsiella tarda / Q000276:immunology*; D004756:Enterobacteriaceae Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D005393:Fish Diseases / Q000097:blood / Q000276:immunology / Q000382:microbiology*; D050990:GATA3 Transcription Factor / Q000097:blood / Q000235:genetics; D007111:Immunity, Cellular / Q000276:immunology; D007371:Interferon-gamma / Q000097:blood / Q000235:genetics; D054353:Perforin / Q000097:blood / Q000235:genetics; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D020825:T-Box Domain Proteins / Q000097:blood / Q000235:genetics; D015854:Up-Regulation / Q000276:immunology; D000098815:T-bet Transcription Factor
chemical_list: D050990:GATA3 Transcription Factor; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D054353:Perforin; D012313:RNA; D007371:Interferon-gamma
keywords: Adoptive transfer; Cell-mediated immunity; Edwarsiella tarda; Ginbuna crucian carp; Intracellular pathogen