title: Radiation-sensitising effects of antennapedia proteins (ANTP)-SmacN7 on tumour cells.
abstract: The objective of this study was to investigate the underlying mechanisms behind the radiation-sensitising effects of the antennapedia proteins (ANTP)-smacN7 fusion protein on tumour cells. ANTP-SmacN7 fusion proteins were synthesised, and the ability of this fusion protein to penetrate cells was observed. Effects of radiation on the expression of X-linked inhibitor of apoptosis protein (XIAP) were detected by western blotting. The radiation-sensitising effects of ANTP-SmacN7 fusion proteins were observed by a clonogenic assay. The effects of drugs and radiation on tumour cell apoptosis were determined using Annexin V/FITC double staining. Changes in caspase-8, caspase-9 and caspase-3 were detected by western blot before and after ANTP-SmacN7 inhibition of XIAP. The ANTP-SmacN7 fusion protein could enter and accumulate in cells; in vitro XIAP expression of radiation-induced tumour cells was negatively correlated with tumour radiosensitivity. The ANTP-SmacN7 fusion protein promoted tumour cell apoptosis through the activation of caspase3. ANTP-SmacN7 fusion protein may reduce tumour cell radioresistance by inducing caspase3 activation.
authors: Li Qing
authors: LQ
authors: Du
authors: Yan
authors: Y
authors: Wang
authors: Chang
authors: C
authors: Xu
authors: Jia
authors: J
authors: Cao
authors: Qin
authors: Q
authors: Wang
authors: Hui
authors: H
authors: Zhao
authors: Fei Yue
authors: FY
authors: Fan
authors: Bing
authors: B
authors: Wang
authors: Takanori
authors: T
authors: Katsube
authors: Sai Jun
authors: SJ
authors: Fan
authors: Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China. liuqiang@irm-cams.ac.cn.
authors: Qiang
authors: Q
authors: Liu
mesh_terms: D000595:Amino Acid Sequence; D051943:Antennapedia Homeodomain Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry; D005720:Gamma Rays; D006367:HeLa Cells; D006801:Humans; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010539:Permeability / Q000187:drug effects / Q000528:radiation effects; D011836:Radiation Tolerance / Q000187:drug effects*; D011838:Radiation-Sensitizing Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D053218:Receptors, Death Domain / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D051943:Antennapedia Homeodomain Protein; D005456:Fluorescent Dyes; D009842:Oligopeptides; D011838:Radiation-Sensitizing Agents; D053218:Receptors, Death Domain; D011993:Recombinant Fusion Proteins; C532637:SMAC peptide; D051636:X-Linked Inhibitor of Apoptosis Protein; D053148:Caspase 3; D053453:Caspase 9