title: Rapid and precise measurement of serum branched-chain and aromatic amino acids by isotope dilution liquid chromatography tandem mass spectrometry.
abstract: BACKGROUND
  Serum branched-chain and aromatic amino acids (BCAAs and AAAs) have emerged as predictors for the future development of diabetes and may aid in diabetes risk assessment. However, the current methods for the analysis of such amino acids in biological samples are time consuming.
  
  
  METHODS
  An isotope dilution liquid chromatography tandem mass spectrometry (ID-LC/MS/MS) method for serum BCAAs and AAAs was developed. The serum was mixed with isotope-labeled BCAA and AAA internal standards and the amino acids were extracted with acetonitrile, followed by analysis using LC/MS/MS. The LC separation was performed on a reversed-phase C18 column, and the MS/MS detection was performed via the positive electronic spray ionization in multiple reaction monitoring mode.
  
  
  RESULTS
  Specific analysis of the amino acids was achieved within 2 min. Intra-run and total CVs for the amino acids were less than 2% and 4%, respectively, and the analytical recoveries ranged from 99.6 to 103.6%.
  
  
  CONCLUSIONS
  A rapid and precise method for the measurement of serum BCAAs and AAAs was developed and may serve as a quick tool for screening serum BCAAs and AAAs in studies assessing diabetes risk.
authors: The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Ministry of Health, Beijing, China.
authors: Ruiyue
authors: R
authors: Yang
authors: Jun
authors: J
authors: Dong
authors: Hanbang
authors: H
authors: Guo
authors: Hongxia
authors: H
authors: Li
authors: Shu
authors: S
authors: Wang
authors: Haijian
authors: H
authors: Zhao
authors: Weiyan
authors: W
authors: Zhou
authors: Songlin
authors: S
authors: Yu
authors: Mo
authors: M
authors: Wang
authors: Wenxiang
authors: W
authors: Chen
mesh_terms: D000328:Adult; D000368:Aged; D024322:Amino Acids, Aromatic / Q000097:blood*; D000597:Amino Acids, Branched-Chain / Q000097:blood*; D001774:Blood Chemical Analysis / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D003920:Diabetes Mellitus / Q000097:blood; D005260:Female; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D011865:Radioisotope Dilution Technique; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors; D055815:Young Adult
chemical_list: D024322:Amino Acids, Aromatic; D000597:Amino Acids, Branched-Chain