title: Investigation of associations between obesity and LEP G2548A and LEPR 668A/G polymorphisms in a Turkish population.
abstract: OBJECTIVE
  Obesity is a complex heterogeneous disease that is caused by genes, environmental factors, and the interaction between the two. The leptin (LEP) and leptin receptor (LEPR) genes have been evaluated for polymorphisms that could potentially be related to the pathophysiology of obesity and its complications. The aim of this study was to investigate the role of LEP G2548A and LEPR 668A/G polymorphisms in the pathogenesis of obesity.
  
  
  METHODS
  The study included 127 patients with obesity and 105 healthy controls. Polymerase chain reaction and restriction fragment length analysis for LEP G2548A and LEPR 668A/G polymorphisms were applied.
  
  
  RESULTS
  There was no statistically significant difference in the genotype frequencies of the LEP gene polymorphism between patients and control groups (P > 0.05). We found a difference in the LEPR genotypes between patients and controls, but this was not statistically significant (P = 0.05). Additionally, we found an increased risk of obesity in the LEP/LEPR GG/GG combined genotype (P < 0.05).
  
  
  CONCLUSIONS
  Our findings indicate that the LEP G2548A polymorphism is not a relevant obesity marker and that the LEPR 668A/G polymorphism may be related to obesity in a Turkish population. Further researches with larger patient population are necessary to ascertain the implications of LEP and LEPR polymorphisms in obesity.
authors: Department of Medical Biology, Faculty of Medicine, Gaziosmanpaşa University, Tokat, Turkey ; Department of Medical Biology, Faculty of Medicine, Tokat and Dumlupinar University, Kütahya, Turkey.
authors: Server
authors: S
authors: Şahın
authors: Aydın
authors: A
authors: Rüstemoğlu
authors: Akın
authors: A
authors: Tekcan
authors: Türker
authors: T
authors: Taşliyurt
authors: Hasan
authors: H
authors: Güven
authors: Serbülent
authors: S
authors: Yığıt
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D020738:Leptin / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D054411:Receptors, Leptin / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014421:Turkey; D055815:Young Adult
chemical_list: D020738:Leptin; D054411:Receptors, Leptin