title: Protective efficacy of a live attenuated Mycoplasma hyopneumoniae vaccine with an ISCOM-matrix adjuvant in pigs.
abstract: An attenuated Mycoplasma hyopneumoniae vaccine that requires intrathoracic administration is commercially available for use against mycoplasmal pneumonia in China. Given the limitations of such a route of administration, this study was undertaken to assess the capacity of an ISCOM-matrix adjuvant to enhance immunogenicity following intramuscular use. Immune responses in pigs following vaccination and subsequent intra-tracheal bacterial inoculation were examined using lymphocyte proliferation, serology and mucosal IgA in both nasal and saliva swabs. Vaccination induced clear lymphocyte proliferation, but only slight serum antibody responses although these were significantly increased following experimental infection. Mucosal IgA was not detected in either nasal or salivary secretions. Following bacterial challenge, animals vaccinated with the adjuvant-containing live vaccine exhibited less severe pulmonary lesions (median score 3.67) than unvaccinated pigs (median score 13.58). The degree of ciliary loss on the respiratory tract surface was reduced in vaccinated pigs compared with experimentally infected controls. The findings indicated that the adjuvant vaccine administered IM provided protection against experimentally induced mycoplasmal pneumonia and could have commercial potential.
authors: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Centre for Engineering Research of Veterinary Bio-products, Nanjing 210014, China.
authors: Qiyan
authors: Q
authors: Xiong
authors: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Centre for Engineering Research of Veterinary Bio-products, Nanjing 210014, China.
authors: Yanna
authors: Y
authors: Wei
authors: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Centre for Engineering Research of Veterinary Bio-products, Nanjing 210014, China.
authors: Zhixin
authors: Z
authors: Feng
authors: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Centre for Engineering Research of Veterinary Bio-products, Nanjing 210014, China.
authors: Yuan
authors: Y
authors: Gan
authors: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Centre for Engineering Research of Veterinary Bio-products, Nanjing 210014, China.
authors: Zhanjun
authors: Z
authors: Liu
authors: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Centre for Engineering Research of Veterinary Bio-products, Nanjing 210014, China.
authors: Maojun
authors: M
authors: Liu
authors: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Centre for Engineering Research of Veterinary Bio-products, Nanjing 210014, China.
authors: Fangfang
authors: F
authors: Bai
authors: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Centre for Engineering Research of Veterinary Bio-products, Nanjing 210014, China. Electronic address: gqshaojaas@gmail.com.
authors: Guoqing
authors: G
authors: Shao
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000009:adverse effects; D005260:Female; D016882:ISCOMs / Q000008:administration & dosage* / Q000276:immunology; D008297:Male; D045705:Mycoplasma hyopneumoniae / Q000276:immunology*; D045729:Pneumonia of Swine, Mycoplasmal / Q000517:prevention & control*; D034421:Sus scrofa; D013552:Swine; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000009:adverse effects
chemical_list: D000276:Adjuvants, Immunologic; D001428:Bacterial Vaccines; D016882:ISCOMs; D014613:Vaccines, Attenuated
keywords: Adjuvant; Intramuscular vaccine; Mycoplasma hyopneumoniae; Pig