title: Protective effect of esculin against prooxidant aflatoxin B1-induced nephrotoxicity in mice.
abstract: The study was designed to investigate the protective effect of esculin against pro-oxidant aflatoxin B1 (AFB1)-induced nephrotoxicity in mice. In this study toxicity was developed by oral administration of AFB1 at a dose of 66.60 μg/kg bw/day for 90 days in male Swiss albino mice. Esculin (150 mg/kg bw/0.2 ml/day) and standard compound ascorbic acid (300 mg/kg bw/0.2 ml/day) was given after 30 min of AFB1 administration for 90 days. Protective efficacy was assessed by measuring the levels of lipid peroxidation (LPO) and non-enzymatic antioxidants such as reduced glutathione (GSH) and also by measuring activities of enzymatic antioxidants such as glutathione peroxidase (GPX), glutathione-S-transferase (GST), glutathione reductase (GR), superoxide dismutase (SOD) and catalase (CAT) in kidney. Results were analysed at the 30(th), 60(th) and 90(th) day of the daily treatments, which showed a decrease in the level of LPO and an increase in the levels of enzymatic and non-enzymatic antioxidants. The protective effect of esculin was further proved by histopathological findings as it exhibited regenerative activities in mice renal tubules against AFB1-induced nephrotoxicity. The results obtained clearly demonstrate that the protective efficacy of esculin against pro-oxidant AFB1-induced nephrotoxicity in mice might be due to its antioxidants and free radical scavenging properties.
authors: Department of Biotechnology, Faculty of Science, Jamia Hamdard, New Delhi, 110062, India.
authors: Farah
authors: F
authors: Naaz
authors: M Z
authors: MZ
authors: Abdin
authors: Saleem
authors: S
authors: Javed
mesh_terms: D000284:Administration, Oral; D016604:Aflatoxin B1 / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000032:analysis; D001205:Ascorbic Acid / Q000008:administration & dosage; D004929:Esculin / Q000008:administration & dosage*; D006651:Histocytochemistry; D007668:Kidney / Q000473:pathology; D015227:Lipid Peroxidation; D008297:Male; D051379:Mice; D011041:Poisoning / Q000517:prevention & control*
chemical_list: D000975:Antioxidants; D004929:Esculin; D016604:Aflatoxin B1; D001205:Ascorbic Acid