title: Inactivation of photosynthetic oxygen evolution by o-phenanthroline and LiClO4 in Photosystem 2 of the pea.
abstract: We examined the effects of o-phenanthroline and LiClO4 on oxygen evolution and electron transport in the Photosystem 2 complex of the pea. Treatment of Photosystem 2 particles with a combination of 3.0 mM o-phenanthroline and 1.0 M LiClO4 for 30-40 min at 0°C decreased the oxygen-evolving activity with the electron acceptor (either phenyl-p-benzoquinone or 2,6-dichlorophenol indophenol) to less than 5% of the original level. However with the same treatment, the electron-transport activity from an artificial electron donor, 1,5-diphenylcarbohydrazide, to 2,6-dichlorophenol indophenol remained at 60% of the original activity. The amount of manganese in the Photosystem 2 complex decreased in parallel with the loss of oxygen evolution following treatment. These observations suggest that the treatment of the Photosystem 2 complex with o-phenanthroline and LiClO4 inhibits electron transport on the oxygen-evolving side much more significantly than on the electron-acceptor side.
authors: National Institute for Basic Biology, Myodaiji, 444, Okazaki, Japan.
authors: S I
authors: SI
authors: Allakhverdiev
authors: H
authors: H
authors: Hayashi
authors: Y
authors: Y
authors: Fujimura
authors: V V
authors: VV
authors: Klimov
authors: N
authors: N
authors: Murata