title: Myeloid-derived suppressor cells enhance IgE-mediated mast cell responses.
abstract: Mast cells and MDSCs are increased by parasitic infection and tumor growth. We previously demonstrated that enhanced MDSC development in ADAM10 transgenic mice yielded resistance to Nb infection and that coculturing MDSCs and mast cells enhanced cytokine production. In the current work, we show that MDSC-mast cell coculture selectively enhances IgE-mediated cytokine secretion among mast cells, without increasing MDSC cytokine production. This effect was independent of cell contact and elicited by Ly6C(+) and Ly6C/G+ MDSC subsets. These interactions were functionally important. MDSC depletion with the FDA-approved drug gemcitabine exacerbated Nb or Trichinella spiralis infection and reduced mast cell-dependent AHR and lung inflammation. Adoptive transfer of MDSC worsened AHR in WT but not mast cell-deficient Wsh/Wsh mice. These data support the hypothesis that MDSCs enhance mast cell inflammatory responses and demonstrate that this interaction can be altered by an existing chemotherapeutic.
authors: 1.Biology Department, Richmond, VA 23284-2012, USA. jjryan@vcu.edu.
authors: Johanna K
authors: JK
authors: Morales
authors: Sheinei J
authors: SJ
authors: Saleem
authors: Rebecca K
authors: RK
authors: Martin
authors: Bryan L
authors: BL
authors: Saunders
authors: Brian O
authors: BO
authors: Barnstein
authors: Travis W
authors: TW
authors: Faber
authors: Nicholas A
authors: NA
authors: Pullen
authors: Elizabeth Motunrayo
authors: EM
authors: Kolawole
authors: Keith B
authors: KB
authors: Brooks
authors: Sarah K
authors: SK
authors: Norton
authors: Jamie
authors: J
authors: Sturgill
authors: Laura
authors: L
authors: Graham
authors: Harry D
authors: HD
authors: Bear
authors: Joseph F
authors: JF
authors: Urban
authors: Chris S
authors: CS
authors: Lantz
authors: Daniel H
authors: DH
authors: Conrad
authors: John J
authors: JJ
authors: Ryan
mesh_terms: D000818:Animals; D001249:Asthma / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D007073:Immunoglobulin E / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D022423:Myeloid Cells / Q000502:physiology*; D009559:Nippostrongylus / Q000276:immunology; D017160:Trichinella spiralis / Q000276:immunology
chemical_list: D016207:Cytokines; D007073:Immunoglobulin E
keywords: Nippostrongylus; Trichinella; allergy; asthma; inflammation