title: Simultaneous determination of capilliposide B and capilliposide C in rat plasma by LC-MS/MS and its application to a PK study.
abstract: BACKGROUND
  Lysimachia capillipes Hemsl (Primulaceae), a folk medicinal plant in China, showed significant anti-tumor activity in recent studies. A reliable LC-MS/MS method was developed and validated for the simultaneous determination of capilliposide B and capilliposide C, the major bioactive components in this plant, in rat plasma.
  
  
  RESULTS
  Rat plasma and whole blood samples were pretreated with dichlorvos, an esterase inhibitor, minimizing degradation of analytes in biological samples. The method validation was conducted over the curve range of 10.0 to 5000 ng/ml for both analytes. The intra- and inter-day precision and accuracy of the QC samples showed ≤6.1% RSD and 1.3-3.7% relative error.
  
  
  CONCLUSIONS
  The method was successfully applied to determine the concentrations of capilliposide B and capilliposide C in incurred rat plasma samples, after administration of Lysimachia capillipes Hemsl extract for a rat PK study.
authors: Tongji School of Pharmacy, Huazhong University of Science & Technology, 13 Hangkong Road, Wuhan 430030, Hubei, China.
authors: Zhongzhe
authors: Z
authors: Cheng
authors: Lin
authors: L
authors: Zhang
authors: Yang
authors: Y
authors: Zhang
authors: Guiying
authors: G
authors: Chen
authors: Hongliang
authors: H
authors: Jiang
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D005260:Female; D057230:Limit of Detection; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012503:Saponins / Q000097:blood* / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014315:Triterpenes / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D012503:Saponins; D014315:Triterpenes; C511099:capilliposide B; C511098:capilliposide C