title: PD-L1 blockade attenuated sepsis-induced liver injury in a mouse cecal ligation and puncture model.
abstract: Liver plays a major role in hypermetabolism and produces acute phase proteins during systemic inflammatory response syndrome and it is of vital importance in host defense and bacteria clearance. Our previous studies indicated that programmed death-1 (PD-1) and its ligand programmed death ligand-1 (PD-L1) are crucial modulators of host immune responses during sepsis. Our current study was designed to investigate the role of PD-L1 in sepsis-induced liver injury by a mouse cecal ligation and puncture (CLP) model. Our results indicated that there was a significant increase of PD-L1 expression in liver after CLP challenge compared to sham-operated controls, in terms of levels of mRNA transcription and immunohistochemistry. Anti-PD-L1 antibody significantly alleviated the morphology of liver injury in CLP mice. Anti-PD-L1 antibody administration decreased ALT and AST release in CLP mice, decreased the levels of tumor necrosis factor (TNF)-α , interleukin (IL)-6, and IL-10 mRNA in liver after sepsis challenge. Thus, anti-PD-L1 antibody might have a therapeutic potential in attenuating liver injury in sepsis.
authors: Department of Anesthesiology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
authors: Weimin
authors: W
authors: Zhu
authors: Rui
authors: R
authors: Bao
authors: Xiaohua
authors: X
authors: Fan
authors: Tianzhu
authors: T
authors: Tao
authors: Jiali
authors: J
authors: Zhu
authors: Jiafeng
authors: J
authors: Wang
authors: Jinbao
authors: J
authors: Li
authors: Lulong
authors: L
authors: Bo
authors: Xiaoming
authors: X
authors: Deng
mesh_terms: D000209:Acute-Phase Proteins / Q000378:metabolism; D000818:Animals; D060890:B7-H1 Antigen / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D002432:Cecum / Q000293:injuries / Q000473:pathology; D005786:Gene Expression Regulation; D007108:Immune Tolerance; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018805:Sepsis / Q000378:metabolism / Q000503:physiopathology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D000209:Acute-Phase Proteins; D060890:B7-H1 Antigen; C498919:Cd274 protein, mouse; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10