title: Use of IPTG-inducible promoters for anchoring recombinant proteins on the Bacillus subtilis spore surface.
abstract: The method of surface display allows the fusion of passenger proteins to a carrier protein displayed on the outside of bioparticles such as spores. Here, we used spores of Bacillus subtilis, the outer surface proteins CotB, CotC, and CotG as carrier and the amyQ-encoded α-amylase and GFPuv as passenger proteins. The different translational fusions were fused to two different IPTG-inducible promoters, and the regulated expression level of both passenger proteins were measured in relation to the inducer concentration added to sporulating cells. It turned out that the amount of fusion protein on the outside of spores was dependent on the amount of IPTG added, but the optimal amount of inducer varied depending on the carrier and the passenger proteins. These experiments demonstrate that a regulatable expression of passenger proteins on the surface of spores is possible. This will help to adjust the amount of any passenger protein to that needed for specific purposes.
authors: Department of Molecular and Environmental Biotechnology, Faculty of Biology, University of Science, Vietnam National University, HCMC, Viet Nam.
authors: Quynh Anh
authors: QA
authors: Nguyen
authors: Institute of Genetics, University of Bayreuth, D-95440 Bayreuth, Germany. Electronic address: wschumann@uni-bayreuth.de.
authors: Wolfgang
authors: W
authors: Schumann
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D007544:Isopropyl Thiogalactoside / Q000737:chemistry* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D013171:Spores, Bacterial / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C439051:CotB protein, Bacillus subtilis; D011993:Recombinant Fusion Proteins; D007544:Isopropyl Thiogalactoside
keywords: CotB; CotC; CotG; IPTG