title: Minor groove binding of the food colorant carmoisine to DNA: spectroscopic and calorimetric characterization studies.
abstract: The interaction of the food additive carmoisine with herring testes DNA was studied by multifaceted biophysical techniques. Carmoisine exhibited hypochromic effects in absorbance, whereas in fluorescence the intensity enhanced upon complexation with DNA. Energy transfer from the DNA base pairs to carmoisine molecules occurred upon complexation. A groove binding model of interaction was envisaged for carmoisine-DNA complexation from 4',6-diamidino-2-phenylindole (DAPI) and Hoechst displacement studies. The binding of carmoisine stabilized the DNA structure against thermal denaturation. The binding induced moderate conformational perturbations in the B-form structure of DNA. The binding affinity (10(4) M(-1)) values, calculated from absorbance and fluorescence data, and calorimetry titrations were in close agreement with each other. The binding was characterized to be exothermic and favored by small negative enthalpic and large positive entropic contributions. Salt-dependent calorimetric studies revealed that the binding reaction was dominated by nonpolyelectrolytic forces. The negative heat capacity value suggested the role of hydrophobic effect in the interaction.
authors: Biophysical Chemistry Laboratory, Chemistry Division, CSIR-Indian Institute of Chemical Biology , Kolkata 700 032, India.
authors: Anirban
authors: A
authors: Basu
authors: Gopinatha Suresh
authors: GS
authors: Kumar
mesh_terms: D002151:Calorimetry; D004247:DNA / Q000737:chemistry*; D005505:Food Coloring Agents / Q000737:chemistry*; D009282:Naphthalenesulfonates / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D013057:Spectrum Analysis
chemical_list: D005505:Food Coloring Agents; D009282:Naphthalenesulfonates; D004247:DNA; C006671:azo rubin S
keywords: DNA binding; carmoisine; groove binder; thermodynamics