title: The influence of serum substituents on serum-free Vero cell conditioned culture media manufactured from Dulbecco's modified Eagle medium in mouse embryo culture.
abstract: OBJECTIVE
  This study was conducted to examine the influences of supplementation of the serum substituents and available period of serum-free Vero cell conditioned media (SF-VCM) manufactured from Dulbecco's modified Eagle medium cultured with Vero cells for in vitro development of mouse preimplantation embryos.
  
  
  METHODS
  A total of 1,099 two-cell embryos collected from imprinting control region mice were cultured in SF-VCM with 10% and 20% human follicular fluid (hFF), serum substitute supplement (SSS), and serum protein substitute (SPS). Development of embryos was observed every 24 hours. Results between different groups were analyzed by chi-square test, and considered statistically significant when P-value was less than 0.05.
  
  
  RESULTS
  The rates of embryonic development cultured in SF-VCM supplemented with serum substituents were significantly higher compare with serum-free group (P < 0.05). The rates of embryonic development after 48 hours (morula≤) and 96 hours (blastocyst≤) were significantly higher in 20% SSS and 10% SPS than in 20% hFF supplementation (P < 0.05). And the rates of embryonic development after 96 hours (hatching blastocyst≤) were significantly higher in 10% SPS (94.5%) than in 20% SSS (82.6%) and 20% hFF supplementation (68.5%). The rates of embryonic development according to storage period of the SF-VCM supplemented with 10% SPS showed no significant difference between control, 2 weeks and 4 weeks group. However developmental rate in 6 weeks storage group was significantly lower than other groups.
  
  
  CONCLUSIONS
  The rate of embryonic development after 96 hours (hatching blastocyst≤) was significantly higher in SF-VCM supplemented with 10% SPS. And storage period of media up to 4 weeks did not affect on embryonic development.
authors: Department of Obstetrics and Gynecology, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon, Korea.
authors: Jong-Seon
authors: JS
authors: Lee
authors: Ju-Hwan
authors: JH
authors: Kim
authors: Young-Seok
authors: YS
authors: Seo
authors: Jung-Bo
authors: JB
authors: Yang
authors: Yong-Il
authors: YI
authors: Kim
authors: Hye-Jin
authors: HJ
authors: Kim
authors: Ki-Hwan
authors: KH
authors: Lee
keywords: Coculture; Mouse embryo; Serum free culture media, Vero cells