title: Relationship of trehalose accumulation with ethanol fermentation in industrial Saccharomyces cerevisiae yeast strains.
abstract: The protective effect and the mechanisms of trehalose accumulation in industrial Saccharomyces cerevisiae strains were investigated during ethanol fermentation. The engineered strains with more intercellular trehalose achieved significantly higher fermentation rates and ethanol yields than their wild strain ZS during very high gravity (VHG) fermentation, while their performances were not different during regular fermentation. The VHG fermentation performances of these strains were consistent with their growth capacity under osmotic stress and ethanol stress, the key stress factors during VHG fermentation. These results suggest that trehalose accumulation is more important for VHG fermentation of industrial yeast strains than regular one. The differences in membrane integrity and antioxidative capacity of these strains indicated the possible mechanisms of trehalose as a protectant under VHG condition. Therefore, trehalose metabolic engineering may be a useful strategy for improving the VHG fermentation performance of industrial yeast strains.
authors: Ocean College, Zhejiang University, Hangzhou 310058, Zhejiang Province, China; Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, Zhejiang Province, China.
authors: Pin-Mei
authors: PM
authors: Wang
authors: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, Zhejiang Province, China.
authors: Dao-Qiong
authors: DQ
authors: Zheng
authors: Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma, Xiamen University Affiliated Zhongshan Hospital, Xiamen 361004, Fujian Province, China.
authors: Xiao-Qin
authors: XQ
authors: Chi
authors: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, Zhejiang Province, China.
authors: Ou
authors: O
authors: Li
authors: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, Zhejiang Province, China.
authors: Chao-Dong
authors: CD
authors: Qian
authors: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, Zhejiang Province, China.
authors: Tian-Zhe
authors: TZ
authors: Liu
authors: State Key Laboratory of Motor Vehicle Biofuel Technology, Nanyang 473000, Henan Province, China.
authors: Xiao-Yang
authors: XY
authors: Zhang
authors: State Key Laboratory of Motor Vehicle Biofuel Technology, Nanyang 473000, Henan Province, China.
authors: Feng-Guang
authors: FG
authors: Du
authors: State Key Laboratory of Motor Vehicle Biofuel Technology, Nanyang 473000, Henan Province, China.
authors: Pei-Yong
authors: PY
authors: Sun
authors: State Key Laboratory of Motor Vehicle Biofuel Technology, Nanyang 473000, Henan Province, China.
authors: Ai-Min
authors: AM
authors: Qu
authors: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, Zhejiang Province, China. Electronic address: mblab@zju.edu.cn.
authors: Xue-Chang
authors: XC
authors: Wu
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D017353:Gene Deletion; D007218:Industrial Microbiology; D042541:Intracellular Space / Q000378:metabolism; D060847:Metabolic Engineering; D050296:Microbial Viability; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000378:metabolism; D014199:Trehalose / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; D014199:Trehalose; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Ethanol; Osmotic stress; Saccharomyces cerevisiae; Trehalose; Very high gravity fermentation