title: A secondary structure in the 5' untranslated region of adhE mRNA required for RNase G-dependent regulation.
abstract: Escherichia coli RNase G is involved in the degradation of several mRNAs, including adhE and eno, which encode alcohol dehydrogenase and enolase respectively. Previous research indicates that the 5' untranslated region (5'-UTR) of adhE mRNA gives RNase G-dependency to lacZ mRNA when tagged at the 5'-end, but it has not been elucidated yet how RNase G recognizes adhE mRNA. Primer extension analysis revealed that RNase G cleaved a phosphodiester bond between -19A and -18C in the 5'-UTR (the A of the start codon was defined as +1). Site-directed mutagenesis indicated that RNase G did not recognize the nucleotides at -19 and -18. Random deletion analysis indicated that the sequence from -145 to -125 was required for RNase G-dependent degradation. Secondary structure prediction and further site-directed deletion suggested that the stem-loop structure, with a bubble in the stem, is required for RNaseG-dependent degradation of adhE mRNA.
authors: Department of Bioengineering, Tokyo Institute of Technology.
authors: Kazutaka
authors: K
authors: Ito
authors: Kohshin
authors: K
authors: Hamasaki
authors: Aya
authors: A
authors: Kayamori
authors: Phuong Anh Thi
authors: PA
authors: Nguyen
authors: Kaoru
authors: K
authors: Amagai
authors: Masaaki
authors: M
authors: Wachi
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000445:Aldehyde Oxidoreductases / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D004722:Endoribonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D007763:Lac Operon / Q000235:genetics; D009690:Nucleic Acid Conformation; D011485:Protein Binding
chemical_list: D020121:5' Untranslated Regions; D029968:Escherichia coli Proteins; D000426:Alcohol Dehydrogenase; C068383:adhE protein, E coli; D000445:Aldehyde Oxidoreductases; D004722:Endoribonucleases; C081905:rng protein, E coli