title: Silencing of TBC1D15 promotes RhoA activation and membrane blebbing.
abstract: Membrane blebs are round-shaped dynamic membrane protrusions that occur under many physiological conditions. Membrane bleb production is primarily controlled by actin cytoskeletal rearrangements mediated by RhoA. Tre2-Bub2-Cdc16 (TBC) domain-containing proteins are negative regulators of the Rab family of small GTPases and contain a highly conserved TBC domain. In this report, we show that the expression of TBC1D15 is associated with the activity of RhoA and the production of membrane blebs. Depletion of TBC1D15 induced activation of RhoA and membrane blebbing, which was abolished by the addition of an inhibitor for RhoA signaling. In addition, we show that TBC1D15 is required for the accumulation of RhoA at the equatorial cortex for the ingression of the cytokinetic furrow during cytokinesis. Our results demonstrate a novel role for TBC1D15 in the regulation of RhoA during membrane blebbing and cytokinesis.
authors: Division of Cancer Biology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
authors: Yuko
authors: Y
authors: Takahara
authors: Masao
authors: M
authors: Maeda
authors: Hitoki
authors: H
authors: Hasegawa
authors: Satoko
authors: S
authors: Ito
authors: Toshinori
authors: T
authors: Hyodo
authors: Eri
authors: E
authors: Asano
authors: Masahide
authors: M
authors: Takahashi
authors: Michinari
authors: M
authors: Hamaguchi
authors: Takeshi
authors: T
authors: Senga
mesh_terms: D045744:Cell Line, Tumor; D048749:Cytokinesis / Q000235:genetics / Q000502:physiology; D020690:GTPase-Activating Proteins / Q000235:genetics* / Q000378:metabolism; D020868:Gene Silencing / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D008566:Membranes / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology; D020742:rhoA GTP-Binding Protein / Q000235:genetics* / Q000378:metabolism
chemical_list: D020690:GTPase-Activating Proteins; C550073:TBC1D15 protein, human; C066737:RHOA protein, human; D020742:rhoA GTP-Binding Protein