title: Evaluation of the protective immunity of a novel subunit fusion vaccine in a murine model of systemic MRSA infection.
abstract: Staphylococcus aureus is a common commensal organism in humans and a major cause of bacteremia and hospital acquired infection. Because of the spread of strains resistant to antibiotics, these infections are becoming more difficult to treat. Therefore, exploration of anti-staphylococcal vaccines is currently a high priority. Iron surface determinant B (IsdB) is an iron-regulated cell wall-anchored surface protein of S. aureus. Alpha-toxin (Hla) is a secreted cytolytic pore-forming toxin. Previous studies reported that immunization with IsdB or Hla protected animals against S. aureus infection. To develop a broadly protective vaccine, we constructed chimeric vaccines based on IsdB and Hla. Immunization with the chimeric bivalent vaccine induced strong antibody and T cell responses. When the protective efficacy of the chimeric bivalent vaccine was compared to that of individual proteins in a murine model of systemic S. aureus infection, the bivalent vaccine showed a stronger protective immune response than the individual proteins (IsdB or Hla). Based on the results presented here, the chimeric bivalent vaccine affords higher levels of protection against S. aureus and has potential as a more effective candidate vaccine.
authors: Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, The Third Military Medical University, Chongqing, People's Republic of China.
authors: Qian-Fei
authors: QF
authors: Zuo
authors: Liu-Yang
authors: LY
authors: Yang
authors: Qiang
authors: Q
authors: Feng
authors: Dong-Shui
authors: DS
authors: Lu
authors: Yan-Dong
authors: YD
authors: Dong
authors: Chang-Zhi
authors: CZ
authors: Cai
authors: Yi
authors: Y
authors: Wu
authors: Ying
authors: Y
authors: Guo
authors: Jiang
authors: J
authors: Gu
authors: Hao
authors: H
authors: Zeng
authors: Quan-Ming
authors: QM
authors: Zou
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D018925:Chemokines / Q000096:biosynthesis; D003001:Cloning, Molecular; D004195:Disease Models, Animal; D007109:Immunity; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009895:Opsonin Proteins / Q000276:immunology; D010587:Phagocytosis; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013203:Staphylococcal Infections / Q000276:immunology* / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D013209:Staphylococcal Vaccines / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D022223:Vaccines, Subunit / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000942:Antigens, Bacterial; D018925:Chemokines; D007074:Immunoglobulin G; D009895:Opsonin Proteins; D011993:Recombinant Fusion Proteins; D013209:Staphylococcal Vaccines; D022223:Vaccines, Subunit