title: Characteristics of long-term cultures of avian primordial germ cells and gonocytes.
abstract: Avian cell lines derived from germinal crescent primordial germ cells and gonadal gonocytes with long-term proliferative capacity in vitro and their subsequent rates of colonization and germline transmission are described. In general, male cultures proliferate more rapidly than female cultures although both can be developed into cell lines of >2 × 10(6) cells, at which time, they can be grown indefinitely and a cell bank can be established. All the cell lines injected into embryos transmitted through the germline with the percentage of germline transmission of both male and female cell lines varying from single digits to the high 90s. The derivation of these primordial germ cell and gonadal cell lines and the subsequent robustness of germline transmission validates these cells as suitable for establishment of lines of chickens bearing novel genetic modifications.
authors: Management of Nature Conservation, Department of the President's Affairs, Abu Dhabi, United Arab Emirates.
authors: Yonghong
authors: Y
authors: Song
authors: Sivakumar
authors: S
authors: Duraisamy
authors: Jahabar
authors: J
authors: Ali
authors: Jaleel
authors: J
authors: Kizhakkayil
authors: Victor Doss
authors: VD
authors: Jacob
authors: Mohammed Ahmed
authors: MA
authors: Mohammed
authors: Mohammed A
authors: MA
authors: Eltigani
authors: Suresh
authors: S
authors: Amisetty
authors: Manoj Kumar
authors: MK
authors: Shukla
authors: Robert J
authors: RJ
authors: Etches
authors: Marie-Cecile van
authors: MC
authors: de Lavoir
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D018929:Cell Culture Techniques / Q000379:methods*; D048430:Cell Shape; D002478:Cells, Cultured; D002642:Chick Embryo; D002678:Chimera / Q000196:embryology; D053595:Embryonic Stem Cells / Q000166:cytology; D005260:Female; D005854:Germ Cells / Q000166:cytology*; D006066:Gonads / Q000166:cytology*; D008297:Male; D013997:Time Factors
keywords: avian; germline transmission; primordial germ cells; transgenic chickens