title: Simultaneous determination of contents of three active components in Jiejia tincture by HPLC method.
abstract: The objective of the study was to determine the contents of three active components in Jiejia tincture by establishing HPLC method. Test articles were prepared by ultrasonic extraction. Separation was performed using a Kromasil C18 (250 mm × 4.6 mm, 5 µm) chromatographic column, and gradient elution was performed with acetonitrile-0.3% phosphoric acid solution as the mobile phase at a volumetric flow rate of 0.80 mL/min. The contents of catechin, baicalin and berberine in Jiejia tincture were determined at the wavelength of 276 nm and a column temperature of 30 □. The results revealed that catechin showed a good linear relationship at the range of 100∼800 µg/mL (r=0.9997); baicalin showed a good linear relationship at the range of 15∼120 µg/mL (r=0.9996), and berberine at the range of 7∼56 µg/mL (r=0.9995). Their average recovery rates were 99.67% (RSD 1.01%, n=6), 98.7% (RSD 1.93%, n=6) and 100.5% (RSD 2.88%, n=6) respectively. The study concluded that the high-performance liquid chromatography established in this study was simple, accurate and reproducible, and can also be used in the determination of catechin, baicalin and berberine contents in Jiejia tincture.
authors: College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, P.R. China.
authors: Li
authors: L
authors: Kun
authors: Liaoning University of Traditional Chinese Medicine, Shenyang 110032, P.R. China.
authors: Wang
authors: W
authors: Fei
authors: College of Pharmacy, Dalian Medical University, Dalian 116044, P.R. China.
authors: Diao
authors: D
authors: Yun-Peng
authors: College of Pharmacy, Dalian Medical University, Dalian 116044, P.R. China.
authors: Pan
authors: P
authors: Xiao-Qiu
authors: Liaoning University of Traditional Chinese Medicine, Shenyang 110032, P.R. China.
authors: E
authors: E
authors: Yun-Juan
authors: College of Pharmacy, Dalian Medical University, Dalian 116044, P.R. China.
authors: Zhang
authors: Z
authors: Huo-Li
mesh_terms: D000045:Acacia / Q000737:chemistry; D001599:Berberine / Q000032:analysis*; D002392:Catechin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D006801:Humans; D019684:Magnoliopsida / Q000737:chemistry*; D032102:Phellodendron / Q000737:chemistry; D008517:Phytotherapy; D015203:Reproducibility of Results; D031364:Scutellaria baicalensis / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D001599:Berberine; C038044:baicalin; D002392:Catechin
keywords: HPLC; Jiejia tincture; baicalin; berberine; catechin