title: Dual role of the caspase enzymes in satellite cells from aged and young subjects.
abstract: Satellite cell (SC) proliferation and differentiation have critical roles in skeletal muscle recovery after injury and adaptation in response to hypertrophic stimuli. Normal ageing hinders SC proliferation and differentiation, and is associated with increased expression of a number of pro-apoptotic factors in skeletal muscle. In light of previous studies that have demonstrated age-related altered expression of genes involved in SC antioxidant and repair activity, this investigation was aimed at evaluating the incidence of apoptotic features in human SCs. Primary cells were obtained from vastus lateralis of nine young (27.3±2.0 years old) and nine old (71.1±1.8 years old) subjects, and cultured in complete medium for analyses at 4, 24, 48, and 72 h. Apoptosis was assessed using AnnexinV/propidium iodide staining, the terminal deoxynucleotidyl transferase dUTP nick-end labelling technique, RT-PCR, DNA microarrays, flow cytometry, and immunofluorescence analysis. There was an increased rate of apoptotic cells in aged subjects at all of the experimental time points, with no direct correlation between AnnexinV-positive cells and caspase-8 activity. On the other hand, CASP2, CASP6, CASP7, and CASP9 and a number of cell death genes were upregulated in the aged SCs. Altogether, our data show age-related enhanced susceptibility of human SCs to apoptosis, which might be responsible for their reduced response to muscle damage.
authors: 1] Department of Neurosciences and Imaging, 'G. d'Annunzio' University, Chieti-Pescara, Italy [2] Stem Tech Group, Centre for Research into Ageing (CeSI), 'G. d'Annunzio' University, Chieti-Pescara, Italy [3] IIM-Interuniversity Institute of Myology, 'G. d'Annunzio' University, Chieti-Pescara, Italy.
authors: S
authors: S
authors: Fulle
authors: 1] Stem Tech Group, Centre for Research into Ageing (CeSI), 'G. d'Annunzio' University, Chieti-Pescara, Italy [2] Department of Pharmacy, 'G. d'Annunzio' University, Chieti-Pescara, Italy.
authors: S
authors: S
authors: Sancilio
authors: 1] Department of Neurosciences and Imaging, 'G. d'Annunzio' University, Chieti-Pescara, Italy [2] Stem Tech Group, Centre for Research into Ageing (CeSI), 'G. d'Annunzio' University, Chieti-Pescara, Italy [3] IIM-Interuniversity Institute of Myology, 'G. d'Annunzio' University, Chieti-Pescara, Italy.
authors: R
authors: R
authors: Mancinelli
authors: 1] Laboratory of Molecular Genetics, Department of Psychological, Humanities and Territorial Sciences, 'G. d'Annunzio' University, Chieti-Pescara, Italy [2] CeSI, Ageing Research Center, 'G. d'Annunzio' University Foundation, Chieti, Italy.
authors: V
authors: V
authors: Gatta
authors: 1] Stem Tech Group, Centre for Research into Ageing (CeSI), 'G. d'Annunzio' University, Chieti-Pescara, Italy [2] Section of Human Morphology, Department of Medicine and Ageing Sciences, 'G. d'Annunzio' University, Chieti-Pescara, Italy.
authors: R
authors: R
authors: Di Pietro
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging / Q000502:physiology*; D017209:Apoptosis / Q000502:physiology; D053143:Caspase 2 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D020169:Caspases / Q000378:metabolism*; D003546:Cysteine Endopeptidases / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D060888:Real-Time Polymerase Chain Reaction; D032496:Satellite Cells, Skeletal Muscle / Q000201:enzymology*
chemical_list: C490360:CASP2 protein, human; D053143:Caspase 2; D053181:Caspase 8; D053453:Caspase 9; D020169:Caspases; D003546:Cysteine Endopeptidases