title: Isolation and molecular characterization of Brazilian turkey reovirus from immunosuppressed young poults.
abstract: In this study, we investigated turkey reovirus (TReoV) in tissue samples from young birds, aged 15 days. RT-PCR for TReoV detected 3.3 % positive samples and TReoV was successfully isolated in Vero cells. Histological analysis of positive bursa of Fabricius (BF) revealed atrophied follicles and lymphocyte depletion. The number of CD8+, CD4+ and IgM+ cells was lower in infected BF. Phylogenetic analysis based on S3 gene showed that the Brazilian TReoV isolates clustered in a single group with 98-100 % similarity to TReoV strains circulating in the United States. This is the first indication that TReoV infection may be a contributing factor to immunosuppression in young birds.
authors: Laboratório de Virologia, Faculdade de Medicina Veterinária, University of São Paulo State, Rua Clóvis Pestana, 793, Araçatuba, SP, CEP 16050-680, Brazil.
authors: Ana Carolina G
authors: AC
authors: Rosa
authors: Helena Lage
authors: HL
authors: Ferreira
authors: Deriane Elias
authors: DE
authors: Gomes
authors: Cilene Vidovix
authors: CV
authors: Táparo
authors: Tereza Cristina
authors: TC
authors: Cardoso
mesh_terms: D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D001938:Brazil; D002060:Bursa of Fabricius / Q000473:pathology / Q000821:virology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002522:Chlorocebus aethiops; D016000:Cluster Analysis; D005838:Genotype; D006651:Histocytochemistry; D016867:Immunocompromised Host; D007075:Immunoglobulin M / Q000276:immunology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D012087:Reoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012088:Reoviridae Infections / Q000662:veterinary* / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014422:Turkeys; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics
chemical_list: D007075:Immunoglobulin M; D012367:RNA, Viral; D014764:Viral Proteins