title: Preferential recognition of avian-like receptors in human influenza A H7N9 viruses.
abstract: The 2013 outbreak of avian-origin H7N9 influenza in eastern China has raised concerns about its ability to transmit in the human population. The hemagglutinin glycoprotein of most human H7N9 viruses carries Leu(226), a residue linked to adaptation of H2N2 and H3N2 pandemic viruses to human receptors. However, glycan array analysis of the H7 hemagglutinin reveals negligible binding to humanlike α2-6-linked receptors and strong preference for a subset of avian-like α2-3-linked glycans recognized by all avian H7 viruses. Crystal structures of H7N9 hemagglutinin and six hemagglutinin-glycan complexes have elucidated the structural basis for preferential recognition of avian-like receptors. These findings suggest that the current human H7N9 viruses are poorly adapted for efficient human-to-human transmission.
authors: Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors: Rui
authors: R
authors: Xu
authors: Robert P
authors: RP
authors: de Vries
authors: Xueyong
authors: X
authors: Zhu
authors: Corwin M
authors: CM
authors: Nycholat
authors: Ryan
authors: R
authors: McBride
authors: Wenli
authors: W
authors: Yu
authors: James C
authors: JC
authors: Paulson
authors: Ian A
authors: IA
authors: Wilson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001717:Birds; D002236:Carbohydrate Conformation; D018360:Crystallography, X-Ray; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D064766:Influenza A Virus, H7N9 Subtype / Q000378:metabolism* / Q000472:pathogenicity*; D005585:Influenza in Birds / Q000635:transmission / Q000821:virology; D007251:Influenza, Human / Q000635:transmission / Q000821:virology; D008024:Ligands; D046228:Microarray Analysis; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D011991:Receptors, Virus / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D008024:Ligands; D011134:Polysaccharides; D011991:Receptors, Virus; D011994:Recombinant Proteins; C109963:hemagglutinin, avian influenza A virus