title: Maturation of cytosolic and nuclear iron-sulfur proteins.
abstract: Eukaryotic cells contain numerous cytosolic and nuclear iron-sulfur (Fe/S) proteins that perform key functions in metabolic catalysis, iron regulation, protein translation, DNA synthesis, and DNA repair. Synthesis of Fe/S clusters and their insertion into apoproteins are essential for viability and are conserved in eukaryotes. The process is catalyzed in two major steps by the CIA (cytosolic iron-sulfur protein assembly) machinery encompassing nine known proteins. First, a [4Fe-4S] cluster is assembled on a scaffold complex. This step requires a sulfur-containing compound from mitochondria and reducing equivalents from an electron transfer chain. Second, the Fe/S cluster is transferred from the scaffold to specific apoproteins by the CIA targeting complex. This review summarizes our molecular knowledge on CIA protein function during the assembly process.
authors: Institut für Zytobiologie, Philipps-Universität Marburg, Robert-Koch-Strasse 6, 35032 Marburg, Germany.
authors: Daili J A
authors: DJ
authors: Netz
authors: Institut für Zytobiologie, Philipps-Universität Marburg, Robert-Koch-Strasse 6, 35032 Marburg, Germany.
authors: Judita
authors: J
authors: Mascarenhas
authors: Institut für Zytobiologie, Philipps-Universität Marburg, Robert-Koch-Strasse 6, 35032 Marburg, Germany.
authors: Oliver
authors: O
authors: Stehling
authors: Institut für Zytobiologie, Philipps-Universität Marburg, Robert-Koch-Strasse 6, 35032 Marburg, Germany.
authors: Antonio J
authors: AJ
authors: Pierik
authors: Institut für Zytobiologie, Philipps-Universität Marburg, Robert-Koch-Strasse 6, 35032 Marburg, Germany; Max-Planck-Institut für Terrestrische Mikrobiologie, Karl-von-Frisch-Strasse 10, 35043 Marburg, Germany; LOEWE (Landes-Offensive zur Entwicklung Wissenschaftlich-Ökonomischer Exzellenz) Zentrum für Synthetische Mikrobiologie (SynMikro), Hans-Meerwein-Strasse, 35043 Marburg, Germany. Electronic address: Lill@staff.uni-marburg.de.
authors: Roland
authors: R
authors: Lill
mesh_terms: D000818:Animals; D001665:Binding Sites; D003600:Cytosol / Q000378:metabolism; D042822:Genomic Instability; D006801:Humans; D007506:Iron-Sulfur Proteins / Q000096:biosynthesis* / Q000502:physiology; D008928:Mitochondria / Q000378:metabolism; D018832:Molecular Chaperones / Q000502:physiology; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000502:physiology; D014176:Protein Biosynthesis; D017510:Protein Folding
chemical_list: D007506:Iron-Sulfur Proteins; D018832:Molecular Chaperones; D009687:Nuclear Proteins
keywords: DNA helicase; DNA polymerases; Iron–sulfur cluster; genome integrity; iron regulation; mitochondrial ISC system