title: Exogenous nucleotides antagonize the developmental toxicity of ethanol in vitro.
abstract: The objective of this study was to assess whether nucleotides supplementation in vitro could suppress ethanol-induced developmental toxicity in mouse. The models of whole embryo culture (WEC) and midbrain (MB) cell micromass culture were used in this study. In WEC system, exposure to 4.0 mg/mL ethanol for 48 h yielded various developmental malformations of the mice embryos. Nucleotides supplementation (0.16, 0.80, 4.00, 20.00, and 100.00 mg/L) improved the growth parameters to some extent, and the protective effects peaked at 4.00 mg/L. In MB cell micromass culture system, exposure to 4.0 mg/mL ethanol for 5 days resulted in suppression of proliferation and differentiation. Supplementation of nucleotides (0.16, 0.80, 4.00, 20.00, and 100.00 mg/L) showed some protective effects, which peaked at 4.00 mg/L, too. The present research indicated that nucleotides supplementation might be of some benefit in the prevention of ethanol-induced birth defects; however, appropriate dosage requires attention.
authors: Department of Nutrition and Food Hygiene, School of Public Health, Peking University, No. 38 Xueyuan Road, Haidian District, Beijing 100191, China.
authors: Jie
authors: J
authors: Zhao
authors: Jia-Xi
authors: JX
authors: Zhao
authors: Ya-Jun
authors: YJ
authors: Xu
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004622:Embryo, Mammalian / Q000187:drug effects / Q000196:embryology; D047108:Embryonic Development / Q000187:drug effects*; D000431:Ethanol / Q000633:toxicity*; D008636:Mesencephalon / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D009711:Nucleotides / Q000494:pharmacology*; D015017:Yolk Sac / Q000166:cytology / Q000187:drug effects
chemical_list: D009711:Nucleotides; D000431:Ethanol