title: Salidroside induces rat mesenchymal stem cells to differentiate into dopaminergic neurons.
abstract: Parkinson's disease (PD) is a neurodegenerative disorder characterised by the loss of substantia nigra dopaminergic neurons that leads to a reduction in striatal dopamine (DA) levels. Replacing lost cells by transplanting dopaminergic neurons has potential value to repair the damaged brain. Salidroside (SD), a phenylpropanoid glycoside isolated from plant Rhodiola rosea, is neuroprotective. We examined whether salidroside can induce mesenchymal stem cells (MSCs) to differentiate into neuron-like cells, and convert MSCs into dopamine neurons that can be applied in clinical use. Salidroside induced rMSCs to adopt a neuronal morphology, upregulated the expression of neuronal marker molecules, such as gamma neuronal enolase 2 (Eno2/NSE), microtubule-associated protein 2 (Map2), and beta 3 class III tubulin (Tubb3/β-tubulin III). It also increased expression of brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and nerve growth factor (NGF) mRNAs, and promoted the secretion of these growth factors. The expression of dopamine neurons markers, such as dopamine-beta-hydroxy (DBH), dopa decarboxylase (DDC) and tyrosine hydroxylase (TH), was significantly upregulated after treatment with salidroside for 1-12 days. DA steadily increased after treatment with salidroside for 1-6 days. Thus salidroside can induce rMSCs to differentiate into dopaminergic neurons.
authors: Institute of Orthopedics, General Hospital of Lanzhou Military Command of the PLA, Lanzhou, Gansu, 730050, China.
authors: Hong-Bin
authors: HB
authors: Zhao
authors: Hui
authors: H
authors: Ma
authors: Xiao-Qin
authors: XQ
authors: Ha
authors: Ping
authors: P
authors: Zheng
authors: Xiao-Yun
authors: XY
authors: Li
authors: Ming
authors: M
authors: Zhang
authors: Ju-Zi
authors: JZ
authors: Dong
authors: Yin-Shu
authors: YS
authors: Yang
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D059290:Dopaminergic Neurons / Q000166:cytology* / Q000378:metabolism; D005960:Glucosides / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism; D020933:Neurotrophin 3 / Q000235:genetics / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D015854:Up-Regulation
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D005960:Glucosides; D008869:Microtubule-Associated Proteins; C079240:Mtap2 protein, mouse; D009414:Nerve Growth Factors; D020933:Neurotrophin 3; D010636:Phenols; D012333:RNA, Messenger; C009172:rhodioloside
keywords: dopaminergic neuron; mesenchymal stem cells; salidroside