title: [The detection of activating somatic mutations in gene KRAS using pyrosequencing technique].
abstract: The technique to detect all possible variants of mutations in 12, 13 and 15 codons of gene KRAS was developed on the basis of the pyrosequencing technology. The analytical characteristics of the developed technique were identified. The limit of detection for mutations G34T, G35A and G38A detected on the cloned control samples consisted 3%. The limit of blank for various mutations consisted from 0.3% to 4.1%. The system was tested on clinical samples. The 7 different types of mutations were identified and detected in quantitative format. No discrepancy of pyrosequencing data with results of sequencing according Sanger was established.
authors: O P
authors: OP
authors: Dribnokhodova
authors: K O
authors: KO
authors: Mironov
authors: E A
authors: EA
authors: Dunaeva
authors: I A
authors: IA
authors: Demidova
authors: A A
authors: AA
authors: Barinov
authors: Ia A
authors: IaA
authors: Boĭtsekhovskaia
authors: M L
authors: ML
authors: Markelov
authors: G A
authors: GA
authors: Shipulin
mesh_terms: D003062:Codon / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D017354:Point Mutation; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins / Q000235:genetics*
chemical_list: D003062:Codon; C117307:KRAS protein, human; D011518:Proto-Oncogene Proteins; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins