title: MicroRNA miR-302 inhibits the tumorigenicity of endometrial cancer cells by suppression of Cyclin D1 and CDK1.
abstract: MicroRNA miR-302 has been found to induce some tumor cell lines to "transdifferentiate" into miRNA-induced pluripotent stem cells (mirPS), thereby inhibiting tumor cell proliferation and reducing tumorigenicity. This study firstly found that miR-302 inhibited the proliferation and migration of endometrial cell line, Ishikawa and HEC-1-B, and arrested cell cycle at the G2/M phase. In addition, miR-302 inhibited tumorigenicity in immunodeficient mice transplanted with Ishikawa cells. Microarray and Western blotting results showed that miR-302 significantly inhibited CDK1 and Cyclin D1 gene expression in Ishikawa cells. MiR-302 directly targeted Cyclin D1, but indirectly regulated CDK1 gene expression.
authors: Center for Reproductive Medicine, the Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical School, Nanjing City 210008, China.
authors: Gui-Jun
authors: GJ
authors: Yan
authors: Center for Experimental Animal, the Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical School, Nanjing City 210008, China.
authors: Fei
authors: F
authors: Yu
authors: Center for Reproductive Medicine, the Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical School, Nanjing City 210008, China.
authors: Bin
authors: B
authors: Wang
authors: Department of Obstetrics and Gynecology, the Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical School, Nanjing City 210008, China.
authors: Huai-Jun
authors: HJ
authors: Zhou
authors: Center for Reproductive Medicine, the Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical School, Nanjing City 210008, China.
authors: Qiu-Yan
authors: QY
authors: Ge
authors: Center for Reproductive Medicine, the Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical School, Nanjing City 210008, China.
authors: Jing
authors: J
authors: Su
authors: Center for Reproductive Medicine, the Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical School, Nanjing City 210008, China.
authors: Ya-Li
authors: YL
authors: Hu
authors: Center for Reproductive Medicine, the Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical School, Nanjing City 210008, China. Electronic address: stevensunz@163.com.
authors: Hai-Xiang
authors: HX
authors: Sun
authors: Center for Reproductive Medicine, the Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical School, Nanjing City 210008, China. Electronic address: xmljding@163.com.
authors: Li-Jun
authors: LJ
authors: Ding
mesh_terms: D000818:Animals; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D019938:Cyclin D1 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D050761:Cyclin-Dependent Kinase Inhibitor p57 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D016889:Endometrial Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D005260:Female; D020869:Gene Expression Profiling; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000008:administration & dosage* / Q000235:genetics; D015398:Signal Transduction; D014162:Transfection / Q000379:methods; D023041:Xenograft Model Antitumor Assays
chemical_list: C493964:CDKN1C protein, human; D050761:Cyclin-Dependent Kinase Inhibitor p57; C532169:MIRN302A microRNA, human; D035683:MicroRNAs; D019938:Cyclin D1
keywords: G2/M phase; HEC-1-B cells; Ishikawa cells; MicroRNA miR-302