title: Crystallization and preliminary X-ray crystallographic analysis of the inhibitory domain of the tomato mosaic virus resistance protein Tm-1.
abstract: Tm-1, an inhibitor protein of Tomato mosaic virus RNA replication, contains two conserved domains: an uncharacterized domain at its N-terminus and a TIM-barrel-like domain at its C-terminus. The N-terminal domain of Tm-1 has an inhibitory activity and its three-dimensional structure has not been determined. Here, the crystallization and preliminary X-ray diffraction of the N-terminal domain of Tm-1 are reported. A three-wavelength MAD data set was collected from a selenomethionine-labelled crystal and processed to 2.7 Å resolution. The crystal belonged to the triclinic space group P1, with unit-cell parameters a = 77.97, b = 105.28, c = 110.62 Å, α = 94.6, β = 109.3, γ = 108.0°.
authors: Biomolecular Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-0856, Japan.
authors: Masahiko
authors: M
authors: Kato
authors: Yuichiro
authors: Y
authors: Kezuka
authors: Chihoko
authors: C
authors: Kobayashi
authors: Kazuhiro
authors: K
authors: Ishibashi
authors: Takamasa
authors: T
authors: Nonaka
authors: Masayuki
authors: M
authors: Ishikawa
authors: Estuko
authors: E
authors: Katoh
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry* / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D018551:Solanum lycopersicum / Q000737:chemistry* / Q000276:immunology / Q000821:virology; D057865:Plant Immunity; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012367:RNA, Viral / Q000037:antagonists & inhibitors / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012645:Selenomethionine / Q000737:chemistry / Q000378:metabolism; D017898:Tobamovirus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D000998:Antiviral Agents; D010940:Plant Proteins; D012367:RNA, Viral; D011994:Recombinant Proteins; D012645:Selenomethionine
keywords: Tm-1; Tomato mosaic virus; replication protein