title: Characterization of variables that may influence ozenoxacin in susceptibility testing, including MIC and MBC values.
abstract: Ozenoxacin is a new des-fluoro-(6)-quinolone active against pathogens involved in skin and skin structure infections, including Gram-positives resistant to fluoroquinolones. The in vitro bacteriostatic and bactericidal activity of ozenoxacin, ciprofloxacin, and levofloxacin was studied against 40 clinical isolates and 16 ATCC quality control strains under different test conditions, including cation supplementation, pH, inoculum size, inoculum preparation, incubation time, human serum, and CO2 incubation. The activity of ozenoxacin was unaffected by cation test medium supplementation, inoculum preparation, incubation time, and the increasing CO2 environment. On the contrary, ozenoxacin activity decreased by high inoculum (10(7) CFU/mL), increased presence of human serum in the medium, and increased pH. The last effect was different for ciprofloxacin and levofloxacin, which decreased activity when pH decreased. The bactericidal mode of action of ozenoxacin and control drugs was consistently maintained (MBC/MIC ratios ≤4) in spite of variations of their activity under different test conditions.
authors: Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.
authors: Marta
authors: M
authors: Tato
authors: Barcelona Centre for International Health Research, (CRESIB), Barcelona, Spain.
authors: Yuly
authors: Y
authors: López
authors: Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.
authors: Maria Isabel
authors: MI
authors: Morosini
authors: Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.
authors: Ana
authors: A
authors: Moreno-Bofarull
authors: Ferrer Laboratories, Barcelona, Spain.
authors: Fernando
authors: F
authors: Garcia-Alonso
authors: Ferrer Laboratories, Barcelona, Spain.
authors: Domingo
authors: D
authors: Gargallo-Viola
authors: Barcelona Centre for International Health Research, (CRESIB), Barcelona, Spain; Servicio de Microbiología, Centro de Diagnóstico Biomédico, Hospital Clínic, Facultad de Medicina, Universidad de Barcelona, Barcelona, Spain.
authors: Jordi
authors: J
authors: Vila
authors: Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain. Electronic address: rafael.canton@salud.madrid.org.
authors: Rafael
authors: R
authors: Cantón
mesh_terms: D000631:Aminopyridines / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial; D024841:Fluoroquinolones / Q000494:pharmacology; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D064704:Levofloxacin / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D015363:Quinolones / Q000494:pharmacology*
chemical_list: D000631:Aminopyridines; D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D015363:Quinolones; D002939:Ciprofloxacin; D064704:Levofloxacin; C000592413:ozenoxacin
keywords: Ozenoxacin; Quinolones; Susceptibility testing; pH effect