title: Rad53 downregulates mitotic gene transcription by inhibiting the transcriptional activator Ndd1.
abstract: The 33 genes in the Saccharomyces cerevisiae mitotic CLB2 transcription cluster have been known to be downregulated by the DNA damage checkpoint for many years. Here, we show that this is mediated by the checkpoint kinase Rad53 and the dedicated transcriptional activator of the cluster, Ndd1. Ndd1 is phosphorylated in response to DNA damage, which blocks recruitment to promoters and leads to the transcriptional downregulation of the CLB2 cluster. Finally, we show that downregulation of Ndd1 is an essential function of Rad53, as a hypomorphic ndd1 allele rescues RAD53 deletion.
authors: Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, USA.
authors: Ellen R
authors: ER
authors: Edenberg
authors: Ajay
authors: A
authors: Vashisht
authors: Jennifer A
authors: JA
authors: Benanti
authors: James
authors: J
authors: Wohlschlegel
authors: David P
authors: DP
authors: Toczyski
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D064447:Checkpoint Kinase 2 / Q000235:genetics / Q000378:metabolism*; D004249:DNA Damage / Q000235:genetics / Q000502:physiology; D015536:Down-Regulation / Q000235:genetics / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008938:Mitosis; D011401:Promoter Regions, Genetic / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D018797:Cell Cycle Proteins; C118820:NDD1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D064447:Checkpoint Kinase 2; C067022:RAD53 protein, S cerevisiae