title: Interaction of cationic carbosilane dendrimers and their complexes with siRNA with erythrocytes and red blood cell ghosts.
abstract: We have investigated the interactions between cationic NN16 and BDBR0011 carbosilane dendrimers with red blood cells or their cell membranes. The carbosilane dendrimers used possess 16 cationic functional groups. Both the dendrimers are made of water-stable carbon-silicon bonds, but NN16 possesses some oxygen-silicon bonds that are unstable in water. The nucleic acid used in the experiments was targeted against GAG-1 gene from the human immunodeficiency virus, HIV-1. By binding to the outer leaflet of the membrane, carbosilane dendrimers decreased the fluidity of the hydrophilic part of the membrane but increased the fluidity of the hydrophobic interior. They induced hemolysis, but did not change the morphology of the cells. Increasing concentrations of dendrimers induced erythrocyte aggregation. Binding of short interfering ribonucleic acid (siRNA) to a dendrimer molecule decreased the availability of cationic groups and diminished their cytotoxicity. siRNA-dendrimer complexes changed neither the fluidity of biological membranes nor caused cell hemolysis. Addition of dendriplexes to red blood cell suspension induced echinocyte formation.
authors: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland. Electronic address: domwro@biol.uni.lodz.pl.
authors: Dominika
authors: D
authors: Wrobel
authors: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
authors: Katarzyna
authors: K
authors: Kolanowska
authors: Department of Thermobiology, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
authors: Arkadiusz
authors: A
authors: Gajek
authors: Departamento Quimica Inorganica, Universidad de Alcala de Henares, Spain.
authors: Rafael
authors: R
authors: Gomez-Ramirez
authors: Departamento Quimica Inorganica, Universidad de Alcala de Henares, Spain.
authors: Javier
authors: J
authors: de la Mata
authors: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
authors: Elżbieta
authors: E
authors: Pedziwiatr-Werbicka
authors: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
authors: Barbara
authors: B
authors: Klajnert
authors: Faculty of Mathematics, Physics and Informatics, Comenius University, Bratislava, Slovakia.
authors: Iveta
authors: I
authors: Waczulikova
authors: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
authors: Maria
authors: M
authors: Bryszewska
mesh_terms: D002412:Cations / Q000737:chemistry; D049109:Cell Proliferation; D050091:Dendrimers / Q000378:metabolism* / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics; D012821:Silanes / Q000737:chemistry* / Q000494:pharmacology; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism
chemical_list: D002412:Cations; D050091:Dendrimers; D034741:RNA, Small Interfering; D012821:Silanes; C504072:carbosilane; D054301:gag Gene Products, Human Immunodeficiency Virus
keywords: Carbosilane; HIV-1; Hemolysis; Membrane fluidity; Red blood cell ghost; siRNA