title: Stress switches cannabinoid type-1 (CB1) receptor-dependent plasticity from LTD to LTP in the bed nucleus of the stria terminalis.
abstract: The bed nucleus of the stria terminalis (BNST) exerts a coordinated modulation of the psychoneuroendocrine responses to stress. However, how acute stress impacts on BNST in vivo plasticity is a crucial question that still remains unanswered. Here, neurons from the anterior portion of the BNST (aBNST) were recorded in vivo during and after stimulation of their medial prefrontal cortical (mPFC) afferents. In C57BL/6N mice, a 1 h restraint stress induced a switch from long-term depression (LTD) to long-term potentiation (LTP) in the aBNST after a 10 Hz mPFC stimulation. This switch was independent from glucocorticoid receptor stimulation. Because the endocannabinoid system regulates aBNST activity, we next examined the role of cannabinoid type-1 receptors (CB1-Rs) in these changes. Mutant mice lacking CB1-Rs (CB1(-/-) mice) displayed a marked deficit in the ability to develop plasticity under control and stress conditions, compared with their wild-type littermates (CB1(+/+) mice). This difference was not accounted for by genetic differences in stress sensitivity, as revealed by Fos immunohistochemistry analyses. Local blockade of CB1-Rs in the aBNST and the use of mutant mice bearing a selective deletion of CB1-Rs in cortical glutamatergic neurons indicated that stress-elicited LTP involved CB1-Rs located on aBNST excitatory terminals. These results show that acute stress reverts LTD into LTP in the aBNST and that the endocannabinoid system plays a key role therein.
authors: UMR 5297 CNRS, Institut Interdisciplinaire de NeuroScience, Development and Adaptation of Neuronal Circuits, Bordeaux F-33076, France, Université Bordeaux Segalen, Bordeaux F-33076, France, and INSERM, U862, Neurocentre Magendie, Pathophysiology of Neuronal Plasticity, Bordeaux F-33076, France.
authors: Christelle
authors: C
authors: Glangetas
authors: Delphine
authors: D
authors: Girard
authors: Laurent
authors: L
authors: Groc
authors: Giovanni
authors: G
authors: Marsicano
authors: Francis
authors: F
authors: Chaouloff
authors: François
authors: F
authors: Georges
mesh_terms: D000818:Animals; D017774:Long-Term Potentiation / Q000502:physiology*; D036881:Long-Term Synaptic Depression / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D009474:Neurons / Q000378:metabolism; D043884:Receptor, Cannabinoid, CB1 / Q000235:genetics / Q000378:metabolism*; D012686:Septal Nuclei / Q000378:metabolism / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology*
chemical_list: D043884:Receptor, Cannabinoid, CB1
keywords: LTD; LTP; corticosterone; endocannabinoid; in vivo