title: [Prediction of the B cell epitopes of hemagglutinin and neuraminidase of human infected avian influenza A H7N9 virus].
abstract: OBJECTIVE
  To predict the B cell epitopes of hemagglutinin (HA) and neuraminidase (NA) of human-infected avian influenza A H7N9 virus by bioinformatics software.
  
  
  METHODS
  The B cell epitopes of HA and NA proteins were predicted comprehensively by bioinformatics software such as Protean, PORTER, MEGA based on the amino acid sequences of HA and NA, and further screened by Wu Yuzhang's average antigen index strategies.
  
  
  RESULTS
  The most possible epitopes of HA were located on its N-terminal No. 53-57, 179-183. The N-terminal No. 62-66, 98-101, 164-166, 315-320, 424-426, 443-445, 493-496 might have the B cell epitopes of HA protein. The most possible epitopes of NA were located on its N-terminal No. 321-329, 379-386. There were possible B cell epitopes located on the N-terminal No. 46-48, 52-54, 74-78, 136-141, 145-150, 160-164, 194-197, 246-248, 333-343, 426-431.
  
  
  CONCLUSIONS
  The prediction of B cell epitopes of HA and NA proteins with multiple methods benefits the research and development of B cell epitope vaccine against human infection with avian influenza A H7N9 virus.
authors: Department of Infectious Diseases, Second Affiliated Hospital, Chongqing Medical University, Ministry of Education Key Laboratory of Molecular Biology for Infectious Diseases, Institute of Viral Heptitis, Chongqing 400010, China.
authors: Hu
authors: H
authors: Li
authors: Peng
authors: P
authors: Hu
mesh_terms: D000595:Amino Acid Sequence; D019295:Computational Biology; D017124:Conserved Sequence; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D064766:Influenza A Virus, H7N9 Subtype / Q000201:enzymology / Q000276:immunology*; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000737:chemistry / Q000276:immunology*; D012984:Software
chemical_list: D018985:Epitopes, B-Lymphocyte; D019267:Hemagglutinin Glycoproteins, Influenza Virus; C109963:hemagglutinin, avian influenza A virus; D009439:Neuraminidase