title: Ultrasound-assisted fabrication of a biocompatible magnetic hydroxyapatite.
abstract: This work describes the fabrication and characterization of a biocompatible magnetic hydroxyapatite (HA) using an ultrasound-assisted co-precipitation method. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and transmission electron microscopy (TEM) were used to characterize the structure and chemical composition of the produced samples. The M-H loops of synthesized materials were traced using a vibrating sample magnetometer (VSM) and the biocompatibility was evaluated by cell culture and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Furthermore, in vivo histopathological examinations were used to evaluate the potential toxicological effects of Fe₃O₄-HA composites on kidney of SD rats injected intraperitoneally with Fe₃O₄-HA particles. The results showed that magnetic iron oxide particles first replace OH ions of HA, which are parallel to the c axis, and then enter the HA crystal lattice which produces changes in the crystal surface of HA. Chemical bond interaction was observed between PO₄³⁻ groups of HA and iron ions of Fe₃O₄. The saturation magnetization (MS ) of Fe₃O₄-HA composites was 46.36 emu/g obtained from VSM data. Cell culture and MTT assays indicated that HA could affect the growth and proliferation of HEK-293 cells. This Fe₃O₄-HA composite produced no negative effects on cell morphology, viability, and proliferation and exhibited remarkable biocompatibility. Moreover, no inflammatory cell infiltration was observed in kidney histopathology slices. Therefore, this study succeeds to develop a Fe₃O₄-HA composite as a prospective biomagnetic material for future applications.
authors: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, People's Republic of China; Research Institute of Beihang University in Shenzhen, Shenzhen, 518057, People's Republic of China.
authors: Gang
authors: G
authors: Zhou
authors: Wei
authors: W
authors: Song
authors: Yongzhao
authors: Y
authors: Hou
authors: Qing
authors: Q
authors: Li
authors: Xuliang
authors: X
authors: Deng
authors: Yubo
authors: Y
authors: Fan
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D048430:Cell Shape / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D017886:Durapatite / Q000494:pharmacology*; D057809:HEK293 Cells; D006801:Humans; D060328:Magnetic Phenomena; D060306:Magnetometry; D008297:Male; D046529:Microscopy, Electron, Transmission; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared; D014465:Ultrasonics / Q000379:methods*; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D017886:Durapatite
keywords: Fe3O4; biocompatibility; composite; hydroxyapatite; magnetic