title: Bacterial flavin mononucleotide riboswitches as targets for flavin analogs.
abstract: Roseoflavin is a toxic riboflavin (vitamin B2) analog and naturally is produced by Streptomyces davawensis. Roseoflavin is converted to roseoflavin mononucleotide (RoFMN) by promiscuous flavokinases (EC 2.7.1.26). Flavin mononucleotide (FMN) riboswitches control the expression of genes involved in riboflavin biosynthesis and/or transport. RoFMN triggers FMN riboswitches and negatively (or positively) affects expression of the downstream genes. RoFMN binding to the aptamer portion of FMN riboswitch RNAs occurs in the course of transcription by cellular RNA polymerases. We developed an in vitro test system to functionally characterize the interaction between riboflavin/FMN analogs such as roseoflavin/RoFMN and FMN riboswitches in the context of an actively transcribing RNA polymerase.
authors: Institute for Technical Microbiology, Mannheim University of Applied Sciences, Mannheim, Germany.
authors: Danielle Biscaro
authors: DB
authors: Pedrolli
authors: Matthias
authors: M
authors: Mack
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005486:Flavin Mononucleotide / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008967:Molecular Biology / Q000379:methods; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D012256:Riboflavin / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D058928:Riboswitch / Q000235:genetics*; D013302:Streptomyces / Q000737:chemistry
chemical_list: D058928:Riboswitch; C009191:roseoflavin; D005486:Flavin Mononucleotide; D017853:Phosphotransferases (Alcohol Group Acceptor); C022532:riboflavin kinase; D012321:DNA-Directed RNA Polymerases; D012256:Riboflavin