title: The experimental study of miRNA in pituitary adenomas.
abstract: OBJECTIVE
  We investigated the differential miRNA expression in pituitary adenomas (both non-functioning and gonadotropin-secreting) and normal pituitaries.
  
  
  METHODS
  RNA was extracted and purified from pituitary adenomas (10 non-functioning and 10 gonadotropin-secreting) and from two normal pituitary tissue samples. The samples were analyzed by miRNA microarray. Gene expression was measured using realtime RT-PCR with SYBR GREEN I.
  
  
  RESULTS
  In non-functioning pituitary adenomas, 25 miRNA genes were up-regulated (six by over 5-fold) and 15 were down-regulated (six by more than 10-fold). miR-124a was the most up-regulated gene (38.58-fold), and miR-31 the most down-regulated gene (21.5-fold). In gonadotropin-secreting pituitary adenomas, 16 miRNA genes were up-regulated (six by over 4-fold) and 13 were down-regulated (seven by more than 10-fold). miR-10b was the most up-regulated gene (48.73-fold), and miR-503 the most down-regulated gene (39.8-fold). Five genes were up-regulated in both subtypes: miR-523, miR-10b, miR-520b, miR-422a, and miR-422b. The RT-PCR results were consistent with those of the gene chips.
  
  
  CONCLUSIONS
  We established miRNA expression maps of non-functioning and gonadotropin-secreting pituitary adenomas. The most strongly differentially expressed genes were miR-124a and miR-31 in non-functioning pituitary adenomas, and miR-10b and miR-503 in gonadotropin-secreting pituitary adenomas.
authors: Tianjin Huanhu Hospital, Department of Neurosurgery, Tianjin, China.
authors: Siquan
authors: S
authors: Liang
authors: Li
authors: L
authors: Chen
authors: Huiling
authors: H
authors: Huang
authors: Dashi
authors: D
authors: Zhi
mesh_terms: D000236:Adenoma / Q000235:genetics / Q000378:metabolism*; D000465:Algorithms; D014408:Biomarkers, Tumor; D002874:Chromosome Mapping; D003627:Data Interpretation, Statistical; D015536:Down-Regulation; D006062:Gonadotropins / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D046228:Microarray Analysis; D010911:Pituitary Neoplasms / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D006062:Gonadotropins; D035683:MicroRNAs