title: Suppression of 2,4-dinitrochlorobenzene-induced atopic dermatitis by extract of Bacillus Calmette-Guerin.
abstract: Bacillus Calmette-Guerin extract (BCGE) has been proven to be clinically effective for anaphylactic disease, infectious diseases and cancer. In this study, we investigated the effect of the intramuscular application of BCGE on 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD). We established an AD model in BALB/c mice by repeated local exposure of DNCB to the ear and dorsal skin. Following intramuscular application of BCGE, the ear thickness, mast cell infiltration, serum total immunoglobulin E (IgE) and histamine level were measured. In addition, levels of interleukin (IL)-4, IL-13, interferon (IFN)-γ and tumor necrosis factor (TNF)-α in the ears were assayed. BCGE reduced AD symptoms based on ear thickness, dermatitis score, histopathological analysis and serum IgE levels. In addition, BCGE inhibited mast cell infiltration into the ear and elevation of serum histamine, increased IFN-γ level and suppressed IL-4, IL-13 and TNF-α levels in the ears. Furthermore, BCGE attenuated the NF-κBp65 expression in the nuclear extract of the ear tissue. Taken together, our results demonstrated that intramuscular application of BCGE exerts beneficial effects on the symptoms of AD suggesting that BCGE may be a candidate for the treatment of AD.
authors: Department of Pharmacology, College of Pharmaceutical Science, China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors: Mingli
authors: M
authors: Sun
authors: Department of Pharmacology, College of Pharmaceutical Science, China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors: Shuang
authors: S
authors: Wang
authors: Department of Pharmacology, College of Pharmaceutical Science, China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors: Lin
authors: L
authors: Zhao
authors: Department of Pharmacology, College of Pharmaceutical Science, China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors: Haishan
authors: H
authors: Zhao
authors: Department of Pharmacology, College of Pharmaceutical Science, China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors: Weifan
authors: W
authors: Yao
authors: Department of Pharmacology, College of Pharmaceutical Science, China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors: Wanbao
authors: W
authors: Jin
authors: Department of Pharmacology, College of Pharmaceutical Science, China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors: Minjie
authors: M
authors: Wei
mesh_terms: D000818:Animals; D001500:BCG Vaccine / Q000008:administration & dosage*; D002457:Cell Extracts / Q000008:administration & dosage*; D003876:Dermatitis, Atopic / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D004137:Dinitrochlorobenzene / Q000633:toxicity; D004195:Disease Models, Animal; D006632:Histamine / Q000097:blood; D007073:Immunoglobulin E / Q000097:blood / Q000187:drug effects; D008407:Mast Cells / Q000187:drug effects / Q000473:pathology; D051379:Mice
chemical_list: D001500:BCG Vaccine; D002457:Cell Extracts; D004137:Dinitrochlorobenzene; D007073:Immunoglobulin E; D006632:Histamine