title: [Protective effect of Rhodiola extract against cisplatin-induced damage to TM4 sertoli cells in mice].
abstract: OBJECTIVE
  To investigate the preventive effect of Rhodiola extract on cisplatin (cDDP)-induced testicular toxicity in mouse TM4 Sertoli cell line and its possible mechanism in vitro.
  
  
  METHODS
  We treated mouse TM4 Sertoli cells with Rhodiola extract and/or cDDP. Then we detected the proliferation of the TM4 cells by MTT assay, observed their morphological changes, and determined the contents of malondialdehyde (MDA), superoxide dismutase (T-SOD) and glutathione (GSH) in the cells.
  
  
  RESULTS
  MTT assay showed that Rhodiola extract at the concentration of 0.0125-2.5 mg/L significantly inhibited the cDDP-induced decrease in the proliferation of the TM4 cells (P < 0.01) and improved their morphological changes. Anti-oxidation test exhibited a dramatically increased level of MDA in the TM4 cells treated with cDDP at 0.0147 g/L as compared with the normal control cells ([3.63 +/- 0.02] vs [2.15 +/- 0.02] nmol/mg prot, P < 0.01) and decreased levels of T-SOD ([6.57 +/- 0.05] vs [10.86 +/- 0.02] U/mg prot, P < 0.01) and GSH ([1.42 +/- 0.06] vs [2.59 +/- 0.05] mg/g prot, P < 0.01). Rhodiola extract at 0.1 mg/L significantly reduced the MDA content ([1.94 +/- 0.00] nmol/mg prot, P < 0.01) and the activity of T-SOD ([8.50 +/- 0.02] U/mg prot, P < 0.01) and GSH ([2.41 +/- 0.04] mg/g prot, P < 0.01) in the TM4 cells treated with cDDP.
  
  
  CONCLUSIONS
  Rhodiola extract can significantly inhibit cDDP-induced damage to TM4 cells in mice, which may be associated with its antioxidant activity.
authors: Beijing Key Laboratory of Biologically Active Substances and Functional Food, Beijing 100191, China. guozhuoyu0310@163.com
authors: Zhuo-Yu
authors: ZY
authors: Guo
authors: Beijing Key Laboratory of Biologically Active Substances and Functional Food, Beijing 100191, China.
authors: Li-Ping
authors: LP
authors: Gao
authors: College of Arts and Science, Beijing Union University, Beijing 100191, China.
authors: Wen-Wen
authors: WW
authors: Li
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002460:Cell Line; D002945:Cisplatin / Q000009:adverse effects*; D005978:Glutathione / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D029771:Rhodiola / Q000737:chemistry*; D012708:Sertoli Cells / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione; D002945:Cisplatin