title: Identification of CD244-expressing myeloid-derived suppressor cells in patients with active tuberculosis.
abstract: Development of active TB is accompanied by immune suppression and the underlining mechanisms have been explored extensively in recent years. MDSCs are a heterogeneous group of immature and progenitor myeloid cells with strong immunosuppressive ability for both natural and adaptive immunity. In our analysis of CD244 (2B4)-expressing cells in PBMCs from patients with active TB, a CD3(-)CD244(high) subpopulation was identified. A match of cell population in flow cytometry showed that nearly all CD3(-)CD244(high) cells were CD3(-)HLA-DR(-)CD11b(int)CD33(+) cells. The CD3(-)CD244(high) cell population has phenotypes of CD3(-)CD19(-)CD56(-)CD15(-)CD66b(-)CD33(+)CD11b(+)CD14(-)HLA-DR(neg/low), which was consistent with MDSCs in humans as previously reported. Patients with active TB had higher frequencies of CD3(-)CD244(high) cells as compared with healthy controls. The CD3(-)CD244(high) cell population had high levels of NOS2 expression and was negatively correlated with activation and effective molecule production of CD4(+) and CD8(+) T cells. In conclusion, CD3(-)CD244(high) cells had phenotypes of MDSCs and CD244 might be used as a marker for human CD3(-)HLA-DR(-)CD11b(int)CD33(+) MDSCs.
authors: Key Laboratory of Tuberculosis Prevention and Treatment of PLA, Division of Research, Institute of Tuberculosis, 309 Hospital, 17 Hei Shan Hu Road, Haidian, Beijing 100091, China.
authors: Bingfen
authors: B
authors: Yang
authors: Key Laboratory of Tuberculosis Prevention and Treatment of PLA, Division of Research, Institute of Tuberculosis, 309 Hospital, 17 Hei Shan Hu Road, Haidian, Beijing 100091, China.
authors: Xinjing
authors: X
authors: Wang
authors: Key Laboratory of Tuberculosis Prevention and Treatment of PLA, Division of Research, Institute of Tuberculosis, 309 Hospital, 17 Hei Shan Hu Road, Haidian, Beijing 100091, China.
authors: Jing
authors: J
authors: Jiang
authors: Key Laboratory of Tuberculosis Prevention and Treatment of PLA, Division of Research, Institute of Tuberculosis, 309 Hospital, 17 Hei Shan Hu Road, Haidian, Beijing 100091, China.
authors: Fei
authors: F
authors: Zhai
authors: Key Laboratory of Tuberculosis Prevention and Treatment of PLA, Division of Research, Institute of Tuberculosis, 309 Hospital, 17 Hei Shan Hu Road, Haidian, Beijing 100091, China. Electronic address: xcheng2@139.com.
authors: Xiaoxing
authors: X
authors: Cheng
mesh_terms: D056704:Adaptive Immunity; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002452:Cell Count; D002478:Cells, Cultured; D018450:Disease Progression; D005260:Female; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007108:Immune Tolerance; D007113:Immunity, Innate; D008297:Male; D023461:Myeloid Progenitor Cells / Q000276:immunology*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D011971:Receptors, Immunologic / Q000378:metabolism*; D000071176:Signaling Lymphocytic Activation Molecule Family; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; C084093:CD244 protein, human; D006684:HLA-DR Antigens; D011971:Receptors, Immunologic; D000071176:Signaling Lymphocytic Activation Molecule Family; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II
keywords: CD244; Immunity; Myeloid-derived suppressor cells; Tuberculosis