title: Multilocus sequence typing system (MLST) reveals a significant association of Entamoeba histolytica genetic patterns with disease outcome.
abstract: The relationship between parasite genotypes and outcome of amoebic infection is still a paradox and needed to be explored. Proper identification and genetic characterization of Entamoeba histolytica clinical isolates is an effective tool for exploring this relation. Along with conventional polymorphic marker (Chitinase), tRNA linked short tandem repeat (STR) loci has been employed as multilocus genotyping tool due to its better resolution and evolutionary significance. Some common as well as exclusive repeat patterns showing significant relation with disease outcome have been identified. Phylogenetic analysis revealed that repeat patterns exclusively found in asymptomatic and amoebic liver abscess derived isolates are placed in a common lineage and has similar association pattern with the disease outcome. Assumption can be made that isolates of E. histolytica remaining asymptomatic is genetically closer siblings of those causing liver abscess rather than the diarrheal isolates.
authors: Division of Parasitology, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme XM, Beliaghata, Kolkata 700010, India. Electronic address: koushikdas55@gmail.com.
authors: Koushik
authors: K
authors: Das
authors: Division of Parasitology, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme XM, Beliaghata, Kolkata 700010, India. Electronic address: aavvik100@gmail.com.
authors: Avik K
authors: AK
authors: Mukherjee
authors: Division of Parasitology, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme XM, Beliaghata, Kolkata 700010, India. Electronic address: punam.bt07@gmail.com.
authors: Punam
authors: P
authors: Chowdhury
authors: Department of Parasitology, Postgraduate Institute of Medical Education & Research, Chandigarh 160012, India.
authors: Rakesh
authors: R
authors: Sehgal
authors: Division of Clinical Medicine, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme XM, Beliaghata, Kolkata 700010, India.
authors: Mihir K
authors: MK
authors: Bhattacharya
authors: Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba 305-8572, Japan. Electronic address: hashimoto.tetsuo.gm@u.tsukuba.ac.jp.
authors: Tetsuo
authors: T
authors: Hashimoto
authors: Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba 305-8572, Japan; Department of Parasitology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan. Electronic address: nozaki@nih.go.jp.
authors: Tomoyoshi
authors: T
authors: Nozaki
authors: Division of Parasitology, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme XM, Beliaghata, Kolkata 700010, India. Electronic address: sandipanganguly@gmail.com.
authors: Sandipan
authors: S
authors: Ganguly
mesh_terms: D016054:DNA, Protozoan / Q000235:genetics*; D004748:Entamoeba histolytica / Q000235:genetics*; D004749:Entamoebiasis / Q000453:epidemiology / Q000469:parasitology* / Q000473:pathology*; D005838:Genotype; D006801:Humans; D007194:India; D008101:Liver Abscess, Amebic / Q000469:parasitology / Q000473:pathology; D058885:Multilocus Sequence Typing; D011110:Polymorphism, Genetic
chemical_list: D016054:DNA, Protozoan
keywords: Amoebic outcome; Chitinase; Entamoeba histolytica; Genotyping; Phylogeny; tRNA linked STR