title: Natural product libraries: assembly, maintenance, and screening.
abstract: This review discusses successful strategies and potential pitfalls to assembling a natural product-based library suitable for high-throughput screening. Specific extraction methods for plants, microorganisms, and marine invertebrates are detailed, along with methods for generating a fractionated sub-library. The best methods to store, maintain and prepare the library for screening are addressed, as well as recommendations on how to develop a robust high-throughput assay. Finally, the logistics of moving from an assay hit to pure bioactive compound are discussed.
authors: Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Brisbane, Australia.
authors: Mark S
authors: MS
authors: Butler
authors: Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Brisbane, Australia.
authors: Frank
authors: F
authors: Fontaine
authors: Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Brisbane, Australia.
authors: Matthew A
authors: MA
authors: Cooper
mesh_terms: D000818:Animals; D001688:Biological Products / Q000627:therapeutic use; D055808:Drug Discovery / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D007448:Invertebrates / Q000737:chemistry; D010936:Plant Extracts / Q000627:therapeutic use; D010944:Plants / Q000737:chemistry; D054852:Small Molecule Libraries
chemical_list: D001688:Biological Products; D010936:Plant Extracts; D054852:Small Molecule Libraries