title: Impaired suppression of feeding by the gut hormone xenin in type I interleukin-1 receptor-deficient mice.
abstract: Xenin is a gut hormone that reduces food intake partly by acting through the hypothalamus. However, the mechanism of hypothalamic xenin action is not fully understood. To identify xenin-regulated genes in the hypothalamus, we compared expression levels of metabolism-related genes in the hypothalamus between saline-treated control and xenin-treated mice. Intraperitoneal injection of xenin caused a significant increase in hypothalamic interleukin 1 beta (IL-1β) mRNA levels without causing a significant change in hypothalamic IL-1α mRNA levels. To further examine the possible contribution of IL-1 signaling to xenin's anorexigenic action, the effect of intraperitoneal injection of xenin on food intake was compared between wild-type and type I IL-1 receptor (IL-1RI)-deficient mice. Intraperitoneal administration of xenin (7.5 μg/g b.w.) caused a significant reduction of food intake in wild-type mice, while it failed to reduce food intake in pre-obese IL-1RI-deficient mice. These findings support the role of hypothalamic IL-1β-IL-1RI signaling in the mediation of the anorexigenic effect of xenin.
authors: Division of Endocrinology & Metabolic Disease, Department of Physiology, University of Manitoba, Winnipeg, MB, Canada.
authors: Eun Ran
authors: ER
authors: Kim
authors: Division of Endocrinology & Metabolic Disease, Department of Physiology, University of Manitoba, Winnipeg, MB, Canada.
authors: Yang
authors: Y
authors: Xu
authors: Division of Endocrinology & Metabolic Disease, Department of Physiology, University of Manitoba, Winnipeg, MB, Canada. Electronic address: mizunot@cc.umanitoba.ca.
authors: Tooru M
authors: TM
authors: Mizuno
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects; D005247:Feeding Behavior / Q000187:drug effects* / Q000502:physiology; D007031:Hypothalamus / Q000187:drug effects* / Q000378:metabolism*; D053590:Interleukin 1 Receptor Antagonist Protein / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D046228:Microarray Analysis; D009496:Neurotensin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D053573:Receptors, Interleukin-1 Type I / Q000172:deficiency* / Q000235:genetics; D015398:Signal Transduction
chemical_list: D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D012333:RNA, Messenger; D053573:Receptors, Interleukin-1 Type I; C077659:xenin 25; D009496:Neurotensin
keywords: Cytokine; Feeding; Gut hormone; Hypothalamus; Obesity