title: [Instability and sensitivity of the genome of healthy children in Magnitogorsk].
abstract: Problem of the influence of factors of the industrial city on the hereditary apparatus of its residents has not been fully resolved, because of traditionally in such studies only the pollution of environment components is taken into account. However the existence of a set of contributing socialfactors that modify the genotoxic effects ofpollution, requires the creation of a new methodology for genetic and toxicological studies. For this purpose, in Magnitogorsk, where one of Russia's largest steel plants is located, we conducted a comprehensive survey, whose tasks included the analysis of the influence of the complex of exogenous and endogenous factors on the genome of children. In this publication there are presented the results of the fifth fragment of this work - the analysis of instability and individual sensitivity of the genome of 166 children of 5-7 years, residing in two districts of Magnitogorsk: around the steel plant and on the opposite bank of Ural river, where there are no large-scale industrial enterprises. The study was conducted in the micronucleus test on peripheral blood lymphocytes cultured with cytochalasin B. For assessment of individual sensitivity of genome blood cultures were exposed to standard N-methyl-N-nitro-N-nitrosoguanidine (MNNG) mutagen. Cytogenetic analysis was performed in binucleated cells accordingly to international protocol, as well as with the use of an extended protocol including 32 indices. Average group frequency of binuclear cells with micronuclei (0.5-0.7%) were found not differ from the levels defined in children residing in Europe, and not differ between areas of the town. However the extended protocol of cytogenetic analysis discovered that the real frequency of dividing cells with lesions in blood cultures of children was 1,49-1,66%. Higher spontaneous proliferative activity of the cells and the frequency of dividing cells with injuries were found in blood cultures of children residing in settlements around the Magnitogorsk steel plant, while the individual sensitivity of the genome in these children was lower than that of their peers from the opposite bank of the Ural river A similar conclusion, but already in relation to the state of the systems for adaptation of entire body has been made from results of other fragments of the study, published earlier.
authors: F I
authors: FI
authors: Ingel'
authors: E K
authors: EK
authors: Krivtsova
authors: N A
authors: NA
authors: Iurtseva
authors: N A
authors: NA
authors: Antipanova
authors: T B
authors: TB
authors: Legostaeva
mesh_terms: D002648:Child; D002675:Child, Preschool; D002947:Cities; D015331:Cohort Studies; D020732:Cytogenetic Analysis; D004781:Environmental Exposure / Q000009:adverse effects*; D005260:Female; D042822:Genomic Instability; D006306:Health Surveys / Q000379:methods*; D006801:Humans; D008297:Male; D056186:Metagenomics / Q000379:methods*; D008669:Metallurgy; D012426:Russia