title: Unusual characteristics of dicistrovirus-derived small RNAs in the small brown planthopper, Laodelphax striatellus.
abstract: In this study, sequences of small RNA (sRNA) libraries derived from the insect vector Laodelphax striatellus were assembled into contigs and used as queries for database searches. A large number of contigs were highly homologous to the genome sequence of an insect dicistrovirus, himetobi P virus (HiPV). Interestingly, HiPV-derived sRNAs had a wide size distribution, and were relatively abundant throughout the 18-30 nt size range with only a slight peak at 22 nt. HiPV sRNAs had a strong bias towards the sense strand, whilst the antisense sRNAs were predominantly 21 and 22 nt. HiPV sRNAs do not have the typical features of PIWI-interacting RNAs, but their 3' ends were preferentially cleaved at UA-rich sequences. Our data suggest that HiPV sRNAs may be derived both from activities of the RNA interference pathway and from cleavage of the viral genome by other host RNases.
authors: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
authors: Junmin
authors: J
authors: Li
authors: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
authors: Ida Bagus
authors: IB
authors: Andika
authors: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, PR China.
authors: Yanru
authors: Y
authors: Zhou
authors: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, PR China.
authors: Jiangfeng
authors: J
authors: Shen
authors: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
authors: Zongtao
authors: Z
authors: Sun
authors: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
authors: Xu
authors: X
authors: Wang
authors: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
authors: Liying
authors: L
authors: Sun
authors: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
authors: Jianping
authors: J
authors: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence; D056629:Dicistroviridae / Q000235:genetics*; D006430:Hemiptera / Q000821:virology*; D007303:Insect Vectors / Q000821:virology*; D008969:Molecular Sequence Data; D058727:RNA, Small Untranslated / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*
chemical_list: D058727:RNA, Small Untranslated; D012367:RNA, Viral