title: Transcriptome sequencing reveals differences between anagen and telogen secondary hair follicle-derived dermal papilla cells of the Cashmere goat (Capra hircus).
abstract: Dermal papilla is considered the control center of hair follicle growth and hair cycle. The secondary hair follicle (producing cashmere) growth cycle of the Cashmere goat (Capra hircus) is circannual, and each growth phase can be easily distinguished by its long duration. To identify gene expression patterns and differences of the dermal papilla cell (DPC) between the anagen and telogen phases, we established two DPC lines: ana-DPCs (DPCs derived from the anagen secondary hair follicle) and tel-DPCs (DPCs derived from the telogen secondary hair follicle). Compared with the ana-DPCs, the tel-DPCs lost the capacity to form cell aggregates and showed lower cell proliferation rate. Transcriptome sequencing revealed that 825 genes were differentially expressed by at least threefold between the two DPC lines. These genes were significantly enriched in cell cycle control, cell division, and chromosome partitioning from the Eukaryotic Orthologous Groups of proteins (KOG) database and in cell cycle, cell adhesion molecules, cytokine-cytokine receptor interaction, and p53 signaling pathway from the Kyoto Encyclopedia of Gene and Genomes (KEGG) database. Enrichment analyses revealed that in the middle of the telogen the DPCs of secondary hair follicles (SHFs) seemed on the one hand to promote the degeneration of SHFs and cessation of cashmere growth, while on the other hand to resist self-apoptosis and prepare for the regeneration or revivification of fully functional dermal papillae. These findings provide a better understanding of hair follicle growth and will be useful for identification of novel molecules associated with the control of hair growth cycle.
authors: The Key Laboratory of Mammalian Reproductive Biology and Biotechnology of the Ministry of Education, Inner Mongolia University, Hohhot, Peoples Republic of China;
authors: Bing
authors: B
authors: Zhu
authors: Teng
authors: T
authors: Xu
authors: Zhipeng
authors: Z
authors: Zhang
authors: Na
authors: N
authors: Ta
authors: Xiaoyu
authors: X
authors: Gao
authors: Lihua
authors: L
authors: Hui
authors: Xudong
authors: X
authors: Guo
authors: Dongjun
authors: D
authors: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D016678:Genome; D006041:Goats; D018859:Hair Follicle / Q000166:cytology* / Q000378:metabolism*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D010507:Periodicity; D011994:Recombinant Proteins / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D059467:Transcriptome
chemical_list: D016207:Cytokines; C583557:IGF1 protein, human; D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I
keywords: dermal papilla cell; hair cycle; signaling pathway; transcriptome sequencing