title: The symbiotic transcription factor MtEFD and cytokinins are positively acting in the Medicago truncatula and Ralstonia solanacearum pathogenic interaction.
abstract: • A plant-microbe dual biological system was set up involving the model legume Medicago truncatula and two bacteria, the soil-borne root pathogen Ralstonia solanacearum and the beneficial symbiont Sinorhizobium meliloti. • Comparison of transcriptomes under symbiotic and pathogenic conditions highlighted the transcription factor MtEFD (Ethylene response Factor required for nodule Differentiation) as being upregulated in both interactions, together with a set of cytokinin-related transcripts involved in metabolism, signaling and response. MtRR4 (Response Regulator), a cytokinin primary response gene negatively regulating cytokinin signaling and known as a target of MtEFD in nodulation processes, was retrieved in this set of transcripts. • Refined studies of MtEFD and MtRR4 expression during M. truncatula and R. solanacearum interaction indicated differential kinetics of induction and requirement of central regulators of bacterial pathogenicity, HrpG and HrpB. Similar to MtRR4, MtEFD upregulation during the pathogenic interaction was dependent on cytokinin perception mediated by the MtCRE1 (Cytokinin REsponse 1) receptor. • The use of M. truncatula efd-1 and cre1-1 mutants evidenced MtEFD and cytokinin perception as positive factors for bacterial wilt development. These factors therefore play an important role in both root nodulation and root disease development.
authors: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, F-31326, Castanet-Tolosan, France.|CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, F-31326, Castanet-Tolosan, France.
authors: Sandra
authors: S
authors: Moreau
authors: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, F-31326, Castanet-Tolosan, France.|CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, F-31326, Castanet-Tolosan, France.
authors: Justine
authors: J
authors: Fromentin
authors: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, F-31326, Castanet-Tolosan, France.|CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, F-31326, Castanet-Tolosan, France.|Université de Toulouse, INP, ENSAT, 18 chemin de Borde Rouge, F-31326, Castanet Tolosan, France.
authors: Fabienne
authors: F
authors: Vailleau
authors: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, F-31326, Castanet-Tolosan, France.|CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, F-31326, Castanet-Tolosan, France.
authors: Tatiana
authors: T
authors: Vernié
authors: Unité de Recherche en Génomique Végétale (URGV), INRA, UMR 1165, Université d'Evry Val d'Essonne, ERL CNRS 8196, CP 5708, F-91057, Evry Cedex, France.
authors: Stéphanie
authors: S
authors: Huguet
authors: Unité de Recherche en Génomique Végétale (URGV), INRA, UMR 1165, Université d'Evry Val d'Essonne, ERL CNRS 8196, CP 5708, F-91057, Evry Cedex, France.
authors: Sandrine
authors: S
authors: Balzergue
authors: Institut des Sciences du Végétal (ISV), Centre National de la Recherche Scientifique (CNRS), 1 avenue de la terrasse, F-91198, Gif-sur-Yvette, France.
authors: Florian
authors: F
authors: Frugier
authors: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, F-31326, Castanet-Tolosan, France.|CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, F-31326, Castanet-Tolosan, France.
authors: Pascal
authors: P
authors: Gamas
authors: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, F-31326, Castanet-Tolosan, France.|CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, F-31326, Castanet-Tolosan, France.|Université de Toulouse, INP, ENSAT, 18 chemin de Borde Rouge, F-31326, Castanet Tolosan, France.
authors: Marie-Françoise
authors: MF
authors: Jardinaud
mesh_terms: D015169:Colony Count, Microbial; D003583:Cytokinins / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D046913:Medicago truncatula / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043368:Ralstonia solanacearum / Q000254:growth & development / Q000472:pathogenicity*; D053204:Root Nodules, Plant / Q000254:growth & development / Q000382:microbiology; D015398:Signal Transduction / Q000235:genetics; D013559:Symbiosis / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D003583:Cytokinins; D010940:Plant Proteins; D012333:RNA, Messenger; D014157:Transcription Factors
keywords: Medicago truncatula; Ralstonia solanacearum; Sinorhizobium meliloti; cytokinins; pathogenesis-symbiosis crosstalk; root pathogen; transcriptome