title: CRIS-a novel cAMP-binding protein controlling spermiogenesis and the development of flagellar bending.
abstract: The second messengers cAMP and cGMP activate their target proteins by binding to a conserved cyclic nucleotide-binding domain (CNBD). Here, we identify and characterize an entirely novel CNBD-containing protein called CRIS (cyclic nucleotide receptor involved in sperm function) that is unrelated to any of the other members of this protein family. CRIS is exclusively expressed in sperm precursor cells. Cris-deficient male mice are either infertile due to a lack of sperm resulting from spermatogenic arrest, or subfertile due to impaired sperm motility. The motility defect is caused by altered Ca(2+) regulation of flagellar beat asymmetry, leading to a beating pattern that is reminiscent of sperm hyperactivation. Our results suggest that CRIS interacts during spermiogenesis with Ca(2+)-regulated proteins that--in mature sperm--are involved in flagellar bending.
authors: Center of Advanced European Studies and Research (caesar), Department of Molecular Sensory Systems, Bonn, Germany.
authors: Anke Miriam
authors: AM
authors: Krähling
authors: Luis
authors: L
authors: Alvarez
authors: Katharina
authors: K
authors: Debowski
authors: Qui
authors: Q
authors: Van
authors: Monika
authors: M
authors: Gunkel
authors: Stephan
authors: S
authors: Irsen
authors: Ashraf
authors: A
authors: Al-Amoudi
authors: Timo
authors: T
authors: Strünker
authors: Elisabeth
authors: E
authors: Kremmer
authors: Eberhard
authors: E
authors: Krause
authors: Ingo
authors: I
authors: Voigt
authors: Simone
authors: S
authors: Wörtge
authors: Ari
authors: A
authors: Waisman
authors: Ingo
authors: I
authors: Weyand
authors: Reinhard
authors: R
authors: Seifert
authors: Ulrich Benjamin
authors: UB
authors: Kaupp
authors: Dagmar
authors: D
authors: Wachten
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics*; D000242:Cyclic AMP / Q000235:genetics* / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D005407:Flagella / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D010766:Phosphorylation; D011485:Protein Binding / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics; D013081:Sperm Motility / Q000235:genetics; D013091:Spermatogenesis / Q000235:genetics*; D013094:Spermatozoa / Q000378:metabolism
chemical_list: C000589447:CRIS protein, mouse; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D000242:Cyclic AMP; D006152:Cyclic GMP; D002118:Calcium