title: Effect of nimesulide on the growth of human laryngeal squamous cell carcinoma.
abstract: OBJECTIVE
  To investigate the effect of nimesulide on the growth of human laryngeal squamous cell carcinoma.
  
  
  METHODS
  The effect of NIM on Hep-2 cell proliferation was measured by the MTT assay. Flow cytometry was used to evaluate the cell cycle and apoptosis in Hep-2 cells. A Western blot analysis was used to detect changes in the protein expression levels of COX-2, Survivin and proliferating cell nuclear antigen (PCNA) in Hep-2 cells. A Hep-2 tumor xenograft model was established in nude mice to observe tumor growth. The changes in the xenograft tumors were observed after hematoxylin/eosin staining. The expression levels of COX-2, Survivin and PCNA proteins and mRNA were measured by immunohistochemical analysis and RT-PCR, respectively.
  
  
  RESULTS
  NIM had time- and dose-dependent inhibitory effect on the proliferation of Hep-2 cells. NIM could prevent the progression of the cell cycle. After NIM treatment, COX-2, Survivin and PCNA protein levels were reduced in the Hep-2 cells. The volume and weight of the xenograft tumors in the NIM treatment group were significantly reduced. The NIM treatment group also exhibited significantly reduced expression levels of COX-2, Survivin and PCNA at both the protein and mRNA levels.
  
  
  CONCLUSIONS
  Our results suggested that NIM has significant inhibitory effects on the growth of Hep-2 cells and xenograft tumors in nude mice. Selective COX-2 inhibitors could potentially become part of a comprehensive treatment for laryngeal squamous cell carcinoma. Additional research and development will provide new and broader prospects for the prevention and treatment of laryngeal squamous cell carcinoma.
authors: Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China.
authors: Zhuoping
authors: Z
authors: Liang
authors: Department of Clinical Laboratory, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China.
authors: Jinbo
authors: J
authors: Liu
authors: Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China.
authors: Leiji
authors: L
authors: Li
authors: Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China.
authors: Haiyang
authors: H
authors: Wang
authors: Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China.
authors: Chong
authors: C
authors: Zhao
authors: Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China.
authors: Liang
authors: L
authors: Jiang
authors: Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China. Electronic address: qing_lzmc@163.com.
authors: Gang
authors: G
authors: Qin
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D051546:Cyclooxygenase 2 / Q000096:biosynthesis / Q000235:genetics; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007150:Immunohistochemistry; D051034:Inhibitor of Apoptosis Proteins / Q000096:biosynthesis / Q000235:genetics; D007822:Laryngeal Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D010975:Platelet Aggregation Inhibitors; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics; D013449:Sulfonamides / Q000494:pharmacology*; D000077022:Survivin
chemical_list: C117749:Birc5 protein, mouse; D016861:Cyclooxygenase Inhibitors; D051034:Inhibitor of Apoptosis Proteins; D010975:Platelet Aggregation Inhibitors; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D012097:Repressor Proteins; D013449:Sulfonamides; D000077022:Survivin; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; C012655:nimesulide