title: Strategies for purifying variants of human rhinovirus 14 2C protein.
abstract: The positive strand RNA genome of picornaviruses, including human rhinovirus (HRV), poliovirus (PV) and foot-and-mouth disease virus, is translated immediately into a polyprotein that is cleaved by virally encoded proteinases into 10-13 mature proteins. These include the four proteins required to assemble the viral particle as well as 3D(pol) (the viral RNA polymerase) and 2C, an ATPase and putative helicase. 2C is a protein which is responsible, together with 2B and 3A, for anchoring the replication complexes to membranous structures in the infected cell on which RNA replication takes place. Additionally, expression of 2C and its precursor 2BC in mammalian cells leads to vesicle formation observed in infected cells. 2C is encoded by all picornaviruses; nevertheless, its exact role in viral replication remains unclear. A contributing factor is the absence of structural data for this hydrophobic protein the generation of which has been hampered by an inability to produce soluble and stable material. Here, we compare 2C from several genera and show that the 2C protein has considerable heterogeneity. Using protein structure meta-analysis, we developed models of HRV14 2C that should be useful for mutational analysis. Based on these analyses, we expressed and purified two domains of HRV14 2C using three different protocols and examined the folding by thermal denaturation or (1)H NMR. Both domains were concentrated sufficiently to allow crystal screens or NMR pilot experiments to be performed. This work provides a platform to explore 2C proteins from all picornaviral genera to generate candidates for structural analysis.
authors: Max F. Perutz Laboratories, Medical University of Vienna, Dept. of Medical Biochemistry, Dr. Bohr-Gasse 9/3, A-1030 Vienna, Austria.
authors: Tomáš
authors: T
authors: Sára
authors: Max F. Perutz Laboratories, University of Vienna, Dept. of Structural and Computational Biology, Campus Vienna Biocenter 5, A-1030 Vienna, Austria.
authors: Robert
authors: R
authors: Konrat
authors: Max F. Perutz Laboratories, Medical University of Vienna, Dept. of Medical Biochemistry, Dr. Bohr-Gasse 9/3, A-1030 Vienna, Austria. Electronic address: timothy.skern@meduniwien.ac.at.
authors: Tim
authors: T
authors: Skern
mesh_terms: D000251:Adenosine Triphosphatases; D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010849:Picornaviridae; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012229:Rhinovirus / Q000235:genetics*; D016415:Sequence Alignment; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins; C076023:2C protein, viral; D000251:Adenosine Triphosphatases
keywords: AAA+ superfamily; Helicase; Insoluble protein purification; Meta-structure analysis; Picornaviruses; Zinc finger