title: LncRNAs expression in preeclampsia placenta reveals the potential role of LncRNAs contributing to preeclampsia pathogenesis.
abstract: BACKGROUND
  Long non-coding RNAs (lncRNAs) are an important class of pervasive genes involved in a variety of biological functions. They are aberrantly expressed in many types of diseases. In this study, we aimed to investigate the lncRNA profiles in preeclampsia. Preeclampsia has been observed in patients with molar pregnancy where a fetus is absent, which demonstrate that the placenta is sufficient to cause this condition. Thus, we analyzed the lncRNA profiles in preeclampsia placentas.
  
  
  RESULTS
  In this study, we described the lncRNA profiles in six preeclampsia placentas (T) and five normal pregnancy placentas (N) using microarray. With abundant and varied probes accounting for 33,045 LncRNAs in our microarray, 28,443 lncRNAs that were expressed at a specific level were detected. From the data, we found 738 lncRNAs that were differentially expressed (≥ 1.5-fold-change) among preeclampsia placentas compared with controls. Coding-non-coding gene co-expression networks (CNC network) were constructed based on the correlation analysis between the differentially expressed lncRNAs and mRNAs. According to the CNC network and GO analysis of differentially expressed lncRNAs/mRNAs, we selected three lncRNAs to analyze the relationship between lncRNAs and preeclampsia. LOC391533, LOC284100, and CEACAMP8 were evaluated using qPCR in 40 preeclampsia placentas and 40 controls. These results revealed that three lncRNAs were aberrantly expressed in preeclampsia placentas compared with controls.
  
  
  CONCLUSIONS
  Our study is the first study to determine the genome-wide lncRNAs expression patterns in preeclampsia placenta using microarray. These results revealed that clusters of lncRNAs were aberrantly expressed in preeclampsia placenta compared with controls, which indicated that lncRNAs differentially expressed in preeclampsia placenta might play a partial or key role in preeclampsia development. Misregulation of LOC391533, LOC284100, and CEACAMP8 might contribute to the mechanism underlying preeclampsia. Taken together, this study may provide potential targets for the future treatment of preeclampsia and novel insights into preeclampsia biology.
authors: Department of Obstetrics, Jiangxi Maternal and Child Health Hospital, Nanchang, China.
authors: Xiaoju
authors: X
authors: He
authors: Yinyan
authors: Y
authors: He
authors: Binrong
authors: B
authors: Xi
authors: Jiusheng
authors: J
authors: Zheng
authors: Xiaoming
authors: X
authors: Zeng
authors: Qinhua
authors: Q
authors: Cai
authors: Yu
authors: Y
authors: Ouyang
authors: Chen
authors: C
authors: Wang
authors: Xiaofei
authors: X
authors: Zhou
authors: Huiying
authors: H
authors: Huang
authors: Wei
authors: W
authors: Deng
authors: Siming
authors: S
authors: Xin
authors: Qixiang
authors: Q
authors: Huang
authors: Huai
authors: H
authors: Liu
mesh_terms: D005260:Female; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000209:etiology* / Q000235:genetics*; D011247:Pregnancy; D062085:RNA, Long Noncoding / Q000235:genetics*; D059467:Transcriptome
chemical_list: D005819:Genetic Markers; D062085:RNA, Long Noncoding