title: [Role of oxidized low-density lipoprotein in NF-κB signal pathway].
abstract: OBJECTIVE
  To explore the effects of oxidized low-density lipoprotein (ox-LDL) on P65, P50, tumor necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6) of human umbilical vein endothelial cells (HUVECs).
  
  
  METHODS
  HUVECs were randomly divided into several groups as follows: the control group, the ox-LDL stimulated groups (different concentrations: 15, 30, 60, 120 μg/mL ox-LDL stimulation for 24 hours, and 30 μg/mL ox-LDL stimulation for different time periods: 12, 24, 48 hours), and the NF-κB inhibitor pyrrolidine dithiocarbamate (PDTC) intervention group (100 μmol/L PDTC pretreatment for 1 h before 30 μg/mL ox-LDL stimulation for 24 hours). Nucleoprotein was extracted to detect P65 and P50 protein expression of NF-κB signaling pathway by Western blotting, and the concentrations of TNF-α and IL-6 in cell supernatants were measured by ELISA.
  
  
  RESULTS
  Western blotting showed that the expressions of P65 and P50 increased under the induction of ox-LDL in a time- and concentration-dependent manner, and the differences were statistically significant among groups (P < 0.05), while they were reduced significantly by PDTC intervention (P < 0.05). ELISA showed that the concentrations of TNF-α and IL-6 in the supernatants of HUVECs stimulated by ox-LDL for 24 hours were significantly higher than those in the control group, while they were reduced significantly by PDTC intervention. There were significant statistical differences among the different treated groups (P < 0.05).
  
  
  CONCLUSIONS
  Ox-LDL can enhance the activity of NF-κB signaling pathway in a time- and concentration-dependent manner through increasing the expressions of TNF-α and IL-6.
authors: Department of Cardiology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China.
authors: Hui
authors: H
authors: Zhai
authors: Yining
authors: Y
authors: Yang
authors: Yitong
authors: Y
authors: Ma
authors: Xiaomei
authors: X
authors: Li
authors: Bangdang
authors: B
authors: Chen
authors: Yang
authors: Y
authors: Xiang
authors: Fen
authors: F
authors: Liu
authors: Zixiang
authors: Z
authors: Yu
authors: Junlin
authors: J
authors: Guo
mesh_terms: D050197:Atherosclerosis / Q000378:metabolism / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D052002:NF-kappa B p50 Subunit / Q000378:metabolism*; D011759:Pyrrolidines / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects*; D013859:Thiocarbamates / Q000494:pharmacology; D013997:Time Factors; D051996:Transcription Factor RelA / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D008077:Lipoproteins, LDL; D052002:NF-kappa B p50 Subunit; D011759:Pyrrolidines; D013859:Thiocarbamates; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; C099252:oxidized low density lipoprotein; C020972:pyrrolidine dithiocarbamic acid