title: Production and characterization of L-fucose dehydrogenase from newly isolated Acinetobacter sp. strain SA-134.
abstract: Microorganisms producing L-fucose dehydrogenase were screened from soil samples, and one of the isolated bacterial strains SA-134 was identified as Acinetobacter sp. by 16S rDNA gene analysis. The strain grew well utilizing L-fucose as a sole source of carbon, but all other monosaccharides tested such as D-glucose and D-arabinose did not support the growth of the strain in the absence of L-fucose. D-Arabinose inhibited the growth even in the culture medium containing L-fucose. Although the strain grew on some organic acids and amino acids such as citric acid and L-alanine as sole sources of carbon, the enzyme was produced only in the presence of L-fucose. The fucose dehydrogenase was purified to apparently homogeneity from the strain, and the native enzyme was a monomer of 25 kD. L-Fucose and D-arabinose were good substrates for the enzyme, but L-galactose was a poor substrate. The enzyme acted on both NAD(+) and NADP(+) in the similar manner.
authors: a Department of Biotechnology , Tottori University , Tottori , Japan.
authors: Takashi
authors: T
authors: Ohshiro
authors: Noriyuki
authors: N
authors: Morita
mesh_terms: D000150:Acinetobacter / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001089:Arabinose / Q000378:metabolism; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005643:Fucose / Q000378:metabolism; D005690:Galactose; D005947:Glucose / Q000378:metabolism; D007218:Industrial Microbiology; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D009243:NAD; D005643:Fucose; D009249:NADP; D001089:Arabinose; D002237:Carbohydrate Dehydrogenases; C020154:L-fucose dehydrogenase; D005947:Glucose; D005690:Galactose