title: Single-center comparison of gel microcolumn and solid-phase methods for antibody screening.
abstract: Our facility changed antibody screening methods from a gel microcolumn-based test (ID-Micro Typing System Gel TEst; Ortho Clinical Diagnostics, Inc., Raritan, NJ) to an automated solid-phase test (Galileo/Capture-R Ready Screen [I and II], Immucor, Inc., Norcross, GA). To determine whether detection rates for commonly encountered clinically significant red blood cell antibodies differed as a consequence of this change, preimplementation and postimplementation antibody identification records were retrospectively reviewed. A statistically significant difference in the percentage of positive screening tests during the gel microcolumn testing period (73,903 total screens, 1.56% confirmed positive) versus the solid0-phase screening period (80,242 total screens, 1.81% confirmed positive; p< 0.0002) was observed . The number of antibodies to K identified was significantly lower with solid phase that with gel (27% decrease; p=0.004). It is unknown whether there is a statistical difference in delayed or hemolytic transfusion reaction rates as this was not evaluated.
authors: MT(ASCP), Division of Transfusion Medicine.
authors: Anne
authors: A
authors: Schmidt
authors: Brenda J
authors: BJ
authors: Bendix
authors: Eapen K
authors: EK
authors: Eapen
authors: Sandra C
authors: SC
authors: Bryant
authors: James R
authors: JR
authors: Stubbs
mesh_terms: D000918:Antibody Specificity; D001787:Blood Group Incompatibility / Q000517:prevention & control*; D001788:Blood Grouping and Crossmatching / Q000706:statistics & numerical data*; D001803:Blood Transfusion; D004912:Erythrocytes / Q000166:cytology / Q000276:immunology*; D057166:High-Throughput Screening Assays / Q000295:instrumentation; D006801:Humans; D007518:Isoantibodies / Q000302:isolation & purification*; D012189:Retrospective Studies
chemical_list: D007518:Isoantibodies