title: Cloning, expression, purification, crystallization and preliminary X-ray studies of argininosuccinate lyase (Rv1659) from Mycobacterium tuberculosis.
abstract: The last enzyme in the arginine-biosynthesis pathway, argininosuccinate lyase, from Mycobacterium tuberculosis has been cloned, expressed, purified and crystallized, and preliminary X-ray studies have been carried out on the crystals. The His-tagged tetrameric enzyme with a subunit molecular weight of 50.9 kDa crystallized with two tetramers in the asymmetric unit of the orthorhombic unit cell, space group P2(1)2(1)2(1). Molecular-replacement calculations and self-rotation calculations confirmed the space group and the tetrameric nature of the molecule.
authors: Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India.
authors: A
authors: A
authors: Paul
authors: A
authors: A
authors: Mishra
authors: A
authors: A
authors: Surolia
authors: M
authors: M
authors: Vijayan
mesh_terms: D001123:Argininosuccinate Lyase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006639:Histidine / Q000737:chemistry / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics; D055503:Protein Multimerization; D021122:Protein Subunits / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C471213:His-His-His-His-His-His; D009842:Oligopeptides; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; D006639:Histidine; D001123:Argininosuccinate Lyase
keywords: Mycobacterium tuberculosis; Rv1659; argininosuccinate lyase