title: Atrazine-based self-assembled monolayers and their interaction with anti-atrazine antibody: building of an immunosensor.
abstract: As a part of our objective to build an immunosensor for the detection of the pesticide atrazine (ATZ) in environmental samples, we studied the self-assembling process of the disulfide derivative of the pesticide atrazine on a gold substrate. Atrazine-based self-assembled monolayers were characterized by ellipsometry, scanning tunneling microscopy, polarization-modulation infrared reflection-absorption spectroscopy (PM IRRAS), X-ray photoelectron spectroscopy and quartz crystal microbalance (QCM) measurements. Two different time constants for the adsorption process were observed, depending on the experimental method used. The QCM data reflect adsorption kinetics of the original disulfide compound, whereas ellipsometry and ex situ PM IRRAS refer to the formation of thiolate (ATZS) monolayers. In situ QCM data demonstrated the suitability of such monolayers for the detection of atrazine in aqueous samples. Exposure of the ATZS sensing surface to an anti-atrazine antibody (anti-ATZ IgG) resulted in complete coverage of the surface by antibody, whereas approximately half of the antibody molecules were displaced from the QCM sensor surface by further addition of atrazine into the solution.
authors: J. Heyrovský Institute of Physical Chemistry of ASCR , v.v.i., Dolejškova 3, 182 23 Prague, Czech Republic.
authors: Magdaléna
authors: M
authors: Hromadová
authors: Lubomír
authors: L
authors: Pospíšil
authors: Romana
authors: R
authors: Sokolová
authors: Jana
authors: J
authors: Bulíčková
authors: Martin
authors: M
authors: Hof
authors: Nathalie
authors: N
authors: Fischer-Durand
authors: Michèle
authors: M
authors: Salmain
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D055910:Antibodies, Immobilized; D001280:Atrazine / Q000737:chemistry*; D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation*; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D000906:Antibodies; D055910:Antibodies, Immobilized; D006046:Gold; D001280:Atrazine