title: Expression and in situ localization of GATA4, 5 and 6 mRNAs in ovine conceptuses and uterine endometria during the peri-implantation period.
abstract: In vertebrates, six GATA transcription factors, GATA1 through GATA6, have been identified and GATA1-3 is known to be involved in hematopoietic developments, while GATA4-6 play roles in cardiac and endoderm developments. Recently, we and others have found that GATA2 and GATA3 found in the trophectoderm plays a role in gene expression specific to this cell type, but GATA4-6 have not been well characterized in early embryonic developments. Using quantitative polymerase chain reaction (qPCR) and in situ hybridization, we examined the expression of GATA4, 5 and 6 messenger RNAs (mRNAs) in ovine conceptuses and uteri during the peri-implantation period. In ovine conceptuses, GATA4, 5 and 6 transcripts were present on days 15, 17 and 21 (day 0 = day of mating), and high GATA5 and 6 mRNAs were found on day 21, most of which were localized in the trophectoderm and endoderm. Moreover, minute and substantial GATA4 and 5 mRNAs were found in days 15 and 21 uterine endometria, respectively. Increase in GATA4-6 transcripts in day 21 uteri indicates that in addition to GATA1-3, GATA4-6 may also play a potentially novel role in the development of ovine trophectoderm, endoderm and/or uterine endometria following conceptus attachment to the uterine epithelium.
authors: Laboratory of Animal Breeding, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors: Hanako
authors: H
authors: Bai
authors: Toshihiro
authors: T
authors: Sakurai
authors: James D
authors: JD
authors: Godkin
authors: Kazuhiko
authors: K
authors: Imakawa
mesh_terms: D000818:Animals; D010064:Embryo Implantation / Q000235:genetics*; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000235:genetics*; D004707:Endoderm / Q000196:embryology*; D004717:Endometrium / Q000196:embryology* / Q000378:metabolism*; D005260:Female; D050991:GATA4 Transcription Factor / Q000235:genetics / Q000502:physiology*; D050992:GATA5 Transcription Factor / Q000235:genetics / Q000502:physiology*; D050993:GATA6 Transcription Factor / Q000235:genetics / Q000502:physiology*; D015870:Gene Expression; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism*; D012756:Sheep / Q000196:embryology* / Q000235:genetics*; D014599:Uterus / Q000196:embryology* / Q000378:metabolism*
chemical_list: D050991:GATA4 Transcription Factor; D050992:GATA5 Transcription Factor; D050993:GATA6 Transcription Factor; D012333:RNA, Messenger
keywords: GATA4-6; gene expression; ovine uterus; peri-implantation period