title: The therapeutic efficacy of I131-PSCA-mAb in orthotopic mouse models of prostate cancer.
abstract: BACKGROUND
  Prostate stem cell antigen (PSCA) is upregulated in prostate cancer tissues. Here we aimed to study the therapeutic efficacy of a monoclonal antibody of PSCA-labeled I131 (I131-PSCA-mAb) in orthotopic mouse models of prostate cancer.
  
  
  METHODS
  The proliferation, apoptosis and invasion abilities of PC-3 and LNCaP cells treated with I131-PSCA-mAb were measured by methyl thiazolyl tetrazolium assay, flow cytometry and transwell culture, respectively. The human prostate cancer models were established by orthotopic implantation of PC-3 and LNCaP cells in nude mice. I131-PSCA-mAb distribution and tumor cell apoptosis in the tumor-bearing nude mice were measured.
  
  
  RESULTS
  The inhibitory and apoptosis rates of PC-3 and LNCaP cells treated with I131-PSCA-mAb reached a maximum of 84%, 80% and 50%, 46%, respectively, which were obviously higher than in the cells treated with I131-IgG or PSCA-mAb. The invaded number of PC-3 and LNCaP cells treated with I131-PSCA-mAbe was significantly reduced (P < 0.01) compared with the control group. The ratios of I131-PSCA-mAb in tumor to intramuscular I131-PSCA-mAb (T/NT) in tumor-bearing nude mice were increased with time and reached the highest level after 8 h. T/NT stayed above 3.0 after 12 h, and the tumor could still be developed after 24 h. The number of apoptotic cells in tumor tissue of nude mice treated with I131-PSCA-mAb was larger than that in the control group.
  
  
  CONCLUSIONS
  I131-PSCA-mAb has the potential to become a new targeted therapy drug for the treatment of prostate cancer.
authors: Shengqiang
authors: S
authors: Yu
authors: Fan
authors: F
authors: Feng
authors: Ke
authors: K
authors: Wang
authors: Changping
authors: C
authors: Men
authors: Chunhua
authors: C
authors: Lin
authors: Qingzuo
authors: Q
authors: Liu
authors: Department of Urology, Yantai Yuhuangding Hospital Affiliated to Medical College of Qingdao University, NO,20 East Yuhuangding Road, 264000 Yantai, China. yangdiandongydd@hotmail.com.
authors: Diandong
authors: D
authors: Yang
authors: Zhenli
authors: Z
authors: Gao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D000951:Antigens, Neoplasm / Q000276:immunology*; D017209:Apoptosis; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D058851:GPI-Linked Proteins / Q000276:immunology; D006801:Humans; D007457:Iodine Radioisotopes; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000276:immunology*; D011471:Prostatic Neoplasms / Q000000981:diagnostic imaging* / Q000188:drug therapy* / Q000473:pathology; D015899:Tomography, Emission-Computed, Single-Photon; D016896:Treatment Outcome
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D058851:GPI-Linked Proteins; D007457:Iodine Radioisotopes; D009363:Neoplasm Proteins; C110843:PSCA protein, human