title: Influence of the peritoneal lavage with bupivacaine on the survival and resistance of colonic anastomoses performed under fecal peritonitis in rats.
abstract: OBJECTIVE
  To evaluate the effects of peritoneal lavage with bupivacaine on survival and initial resistance of anastomosis on distal colon, performed under peritonitis or not.
  
  
  METHODS
  Forty rats, weighing from 300 to 350 g (321.29 ± 11.3 1g), were randomly divided in four groups and underwent laparotomy and anastomosis on the distal colon six hours after induction of peritonitis by intraperitoneal injection of autologous fecal material or not. Group 1: No peritonitis and lavage with 3 ml NS; Group 2: No peritonitis and lavage with 8 mg.kg-1 (± 0.5 mL) of 0.5% bupivacaine added to 2.5 mL of NS; Group 3: Peritonitis and lavage with 3 ml NS; Group 4: Peritonitis and lavage with 8 mg.kg-1 (± 0.5 mL) of 0.5% bupivacaine added to 2.5 mL of NS. Necropsies were performed on the animals that died and the time of death was recorded. Surviving animals were submitted to euthanasia on the fifth post-operative day and Total Energy of Rupture biomechanical test (TER) was applied.
  
  
  RESULTS
  Group 4 showed survival increase compared to Group 3, without statistical significance. Group 3 presented the smallest average TER, with statistical significance.
  
  
  CONCLUSIONS
  Peritonitis increased mortality and influenced negatively the resistance of colic anastomosis in rats. Peritoneal lavage with bupivacaine increased anastomotic resistance.
authors: Michel Gardere
authors: MG
authors: Camargo
authors: João José
authors: JJ
authors: Fagundes
authors: Raquel Franco
authors: RF
authors: Leal
authors: Maria de Lourdes Setsuko
authors: Mde L
authors: Ayrizono
authors: Débora Helena dos Gonçalves
authors: DH
authors: Rossi
authors: Priscilla de Sene Portel
authors: Pde S
authors: Oliveira
authors: Wu Feng
authors: WF
authors: Chung
authors: Huei Diana
authors: HD
authors: Lee
authors: Cláudio Saddy Rodrigues
authors: CS
authors: Coy
mesh_terms: D000714:Anastomosis, Surgical; D000779:Anesthetics, Local / Q000627:therapeutic use*; D000818:Animals; D002045:Bupivacaine / Q000627:therapeutic use*; D003106:Colon / Q000601:surgery*; D004195:Disease Models, Animal; D005243:Feces; D008297:Male; D010533:Peritoneal Lavage / Q000379:methods*; D010538:Peritonitis / Q000401:mortality / Q000628:therapy*; D011184:Postoperative Period; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D015996:Survival Rate; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D000779:Anesthetics, Local; D002045:Bupivacaine