title: The intrinsic dynamics of Cse1p and Xpot elucidated by coarse-grained models.
abstract: Cse1p and Xpot are two karyopherin proteins that transport the corresponding cargos during the nucleocytoplasmic transport. We utilized Elastic Network Model (ENM) and Finite Element Analysis (FEA) to study their conformational dynamics. These dynamics were interpreted by their intrinsic modes that played key roles in the flexibility of karyopherins, which further affected the binding affinities. The findings included that it was the karyopherin's versatile conformations composed of the same superhelices of HEAT repeats that produced different degrees of functional flexibilities. We presented evidence that these coarse-grained methods could help to elucidate the biological function behind the structures of the two karyopherins.
authors: Mechanical and Industrial Engineering, University of Massachusetts, Amherst, MA 01003, USA.
authors: Ming-Wen
authors: MW
authors: Hu
authors: Mechanical and Industrial Engineering, University of Massachusetts, Amherst, MA 01003, USA.
authors: Chiung-Fang
authors: CF
authors: Hsu
authors: Mechanical and Industrial Engineering, University of Massachusetts, Amherst, MA 01003, USA. Electronic address: kim@ecs.umass.edu.
authors: Byung
authors: B
authors: Kim
mesh_terms: D029563:Cellular Apoptosis Susceptibility Protein / Q000737:chemistry*; D020342:Finite Element Analysis; D008958:Models, Molecular; D029543:Nucleocytoplasmic Transport Proteins / Q000737:chemistry*; D011487:Protein Conformation
chemical_list: D029563:Cellular Apoptosis Susceptibility Protein; D029543:Nucleocytoplasmic Transport Proteins; C503618:XPOT protein, human
keywords: Elastic Network Model; Finite Element Analysis; Karyopherin; Nucleocytoplasmic transport; Protein flexibility