title: Airway contractility in the precision-cut lung slice after cryopreservation.
abstract: An emerging tool in airway biology is the precision-cut lung slice (PCLS). Adoption of the PCLS as a model for assessing airway reactivity has been hampered by the limited time window within which tissues remain viable. Here we demonstrate that the PCLS can be frozen, stored long-term, and then thawed for later experimental use. Compared with the never-frozen murine PCLS, the frozen-thawed PCLS shows metabolic activity that is decreased to an extent comparable to that observed in other cryopreserved tissues but shows no differences in cell viability or in airway caliber responses to the contractile agonist methacholine or the relaxing agonist chloroquine. These results indicate that freezing and long-term storage is a feasible solution to the problem of limited viability of the PCLS in culture.
authors: 1 Department of Environmental Health, Harvard School of Public Health, Boston, Massachusetts.
authors: Sonia R
authors: SR
authors: Rosner
authors: Sumati
authors: S
authors: Ram-Mohan
authors: Jesus R
authors: JR
authors: Paez-Cortez
authors: Tera L
authors: TL
authors: Lavoie
authors: Maria L
authors: ML
authors: Dowell
authors: Lei
authors: L
authors: Yuan
authors: Xingbin
authors: X
authors: Ai
authors: Alan
authors: A
authors: Fine
authors: William C
authors: WC
authors: Aird
authors: Julian
authors: J
authors: Solway
authors: Jeffrey J
authors: JJ
authors: Fredberg
authors: Ramaswamy
authors: R
authors: Krishnan
mesh_terms: D000818:Animals; D016923:Cell Death / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D015925:Cryopreservation / Q000379:methods; D005615:Freezing; D008168:Lung / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009119:Muscle Contraction / Q000502:physiology*