title: FAF and SufA: proteins of Finegoldia magna that modulate the antibacterial activity of histones.
abstract: Many bacterial pathogens have developed methods to overcome the defences of the host innate immune system. One such defence is the release of antimicrobial peptides (AMPs). Histones have been found to function as AMPs, in addition to their main biological function of packaging and organising DNA into nucleosomes. In this study, the Gram-positive anaerobic coccus Finegoldia magna was found to bind histones by Western blot and immunoprecipitation analysis. F. magna, which is normally a commensal of the skin and mucous membranes, is also known to act as an opportunistic pathogen and has been isolated from various clinical infection sites. It was found to bind to histones extracted from human skin epidermis through its surface and extracellular adhesion protein FAF. Through FAF binding, F. magna was protected from histone bactericidal activity. Furthermore, the histones were found to be degraded by SufA, a subtilisin-like extracellular serine protease of F. magna. Hence, the results of the present study will give more insight into how F. magna persists both as a commensal organism at the basement membrane of the skin and as an opportunistic pathogen during infection.
authors: Division of Infection Medicine, Department of Clinical Sciences, Lund University, Lund, Sweden.
authors: Elizabeth C
authors: EC
authors: Murphy
authors: Tirthankar
authors: T
authors: Mohanty
authors: Inga-Maria
authors: IM
authors: Frick
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism*; D004817:Epidermis / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D016908:Gram-Positive Bacterial Infections / Q000235:genetics / Q000276:immunology*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D056747:Immunomodulation; D064307:Microbiota; D009092:Mucous Membrane / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D010462:Peptostreptococcus / Q000235:genetics / Q000276:immunology*; D011485:Protein Binding; D059748:Proteolysis; D020860:Subtilisin / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D006657:Histones; D020860:Subtilisin