title: Fluorescent immunosensor based on CuS nanoparticles for sensitive detection of cancer biomarker.
abstract: A novel fluorescent immunosensor was developed based on the use of CuS nanoparticles (CuS NPs) as labels for the highly sensitive detection of human prostate cancer biomarker prostate specific antigen (PSA). In the presence of CuS NPs, the non-fluorescent substrate o-phenylenediamine could be oxidized into the stable fluorescent product 2,3-diamiophenazine at physiological pH. Throughout the reaction, no other oxidizing agents (e.g. hydrogen peroxide) were needed. The relatively mild oxidation conditions made the immunoassay robust, reliable and facile. The proposed immunoassay exhibited high sensitivity and specificity for the detection of PSA. A linear relationship between the fluorescent signals and the concentration of PSA was obtained in the range of 0.5 pg mL(-1) to 50 ng mL(-1), with a detection limit of 0.1 pg mL(-1) (S/N = 3). The proposed fluorescent immunoassay can be used as a promising platform for the detection of a variety of other biomarkers.
authors: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P. R. China. jianglp@nju.edu.cn.
authors: Ying-Di
authors: YD
authors: Zhu
authors: Juan
authors: J
authors: Peng
authors: Li-Ping
authors: LP
authors: Jiang
authors: Jun-Jie
authors: JJ
authors: Zhu
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood*; D015374:Biosensing Techniques / Q000379:methods*; D003300:Copper / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000379:methods*; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D017430:Prostate-Specific Antigen / Q000097:blood; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
chemical_list: D000906:Antibodies; D014408:Biomarkers, Tumor; D003300:Copper; D017430:Prostate-Specific Antigen