title: In vitro and in vivo effects of neem tree (Azadirachta indica A. Juss) products on larvae of the sheep nose bot fly (Oestrus ovis L. Díptera: Oestridae).
abstract: Two studies were carried out in order to test the effects of neem tree extracts (Azadirachta indica A. Juss) on sheep bot fly larvae (Oestrus ovis L. Diptera: Oestridae). First, aqueous extracts from neem seeds (ASNE) at 0, 5 y 10% (w/v) concentrations were tested on larval mortality in vitro. In a second study, the effect of oral administration with neem seed meal (0, 100 y 200mg/kg) and neem leaves (1% of diet) on number of larvae found at necropsy and larval development was evaluated in experimentally O. ovis-infected sheep. Results in Experiment 1 showed a significant (P<0.05) effect of ASNE on time to L1 mortality in a dosis-dependent manner. In Experiment 2, oral administration of seeds or leaves did not affect the number of larvae found at necropsy of the sheep, but interfered with larval development and there was a tendency to reduce larval weight at the end of the infection period (55d).
authors: Departamento de Zootecnia, Universidad Autónoma de Baja California Sur, Km. 5.5 carr. al Sur. A.P. 19-B, C.P. 23080 La Paz, B.C.S., Mexico.
authors: R
authors: R
authors: Cepeda-Palacios
authors: Centro de Investigaciones Biológicas del Noroeste, S.C. Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, C.P. 23096 La Paz, B.C.S., Mexico.
authors: R
authors: R
authors: Servín
authors: Departamento de Zootecnia, Universidad Autónoma de Baja California Sur, Km. 5.5 carr. al Sur. A.P. 19-B, C.P. 23080 La Paz, B.C.S., Mexico.
authors: J M
authors: JM
authors: Ramírez-Orduña
authors: Centro de Investigaciones Biológicas del Noroeste, S.C. Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, C.P. 23096 La Paz, B.C.S., Mexico.
authors: F
authors: F
authors: Ascencio
authors: Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076, Toulouse Cedex 03, France.
authors: P
authors: P
authors: Dorchies
authors: Centro de Investigaciones Biológicas del Noroeste, S.C. Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, C.P. 23096 La Paz, B.C.S., Mexico. Electronic address: eangulo@cibnor.mx.
authors: C E
authors: CE
authors: Angulo-Valadez
mesh_terms: D000818:Animals; D031601:Azadirachta / Q000737:chemistry*; D004175:Diptera / Q000187:drug effects*; D007814:Larva / Q000187:drug effects; D009198:Myiasis / Q000188:drug therapy / Q000662:veterinary*; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use*; D018515:Plant Leaves; D012639:Seeds / Q000737:chemistry; D012756:Sheep; D012757:Sheep Diseases / Q000188:drug therapy*; D016896:Treatment Outcome
chemical_list: D010936:Plant Extracts
keywords: Larval development; Larval mortality; Neem (Azadirachta indica A. Juss); O. ovis; Sheep