title: Analysis of organ-enriched microRNAs in plasma as an approach to development of Universal Screening Test: feasibility study.
abstract: BACKGROUND
  Early disease detection with a minimally invasive screening test will significantly increase effectiveness and decrease the cost of treatment. Here we propose a framework of a novel approach - Universal Screening Test (UST) for the detection of pathological processes in a particular organ system, organ, or tissue by RT-qPCR analysis of circulating cell-free miRNAs in plasma. As the first step towards assessing the feasibility of this concept, the present study was designed to analyze whether the same microRNAs (miRNAs) can detect various diseases of a particular organ system.
  
  
  METHODS
  RNA was extracted from plasma using Trizol treatment and silica binding. Levels of miRNAs were measured by single target RT-qPCR. The following innovations have been tested and proven effective: (i) the use of organ system/organ/tissue-enriched miRNAs; (ii) the use of miRNAs associated with broad disease categories, such as cancer and inflammation, in combination with the organ-enriched miRNAs; and (iii) the use of "miRNA pairs" for selecting miRNA combinations with the highest sensitivity and specificity.
  
  
  RESULTS
  Here we report biomarker miRNA pairs effectively differentiating (i) patients with pulmonary system diseases (asthma, pneumonia and non-small cell lung cancer) and gastrointestinal (GI) system diseases (Crohn's disease, stages I/II esophageal, gastric and colon cancers) from controls, each with 95% accuracy; (ii) patients with a pathology of the pulmonary system from patients with a pathology of the GI system with 94% accuracy; and (iii) cancer patients (stages I/II esophageal, gastric, colon cancers, or non-small cell lung cancer) from patients with inflammatory diseases (asthma, pneumonia, or Crohn's disease) with 93%-95% accuracy.
  
  
  CONCLUSIONS
  The results obtained in the present study, along with the data reported by us and others previously, are encouraging and lay the ground for further investigation of the described approach for UST development.
authors: Kira S
authors: KS
authors: Sheinerman
authors: Vladimir G
authors: VG
authors: Tsivinsky
authors: DiamiR, LLC, 11 Deer Park Drive, Suite 102G, Monmouth Junction, NJ 08852, USA. sumansky@diamirbio.com.
authors: Samuil R
authors: SR
authors: Umansky
mesh_terms: D042241:Early Diagnosis; D005240:Feasibility Studies; D005767:Gastrointestinal Diseases / Q000175:diagnosis*; D006801:Humans; D035683:MicroRNAs / Q000097:blood*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D035683:MicroRNAs