title: Structural insights into RbmA, a biofilm scaffolding protein of V. cholerae.
abstract: V. cholerae can form sessile biofilms associated with abiotic surfaces, cyanobacteria, zoo-plankton, mollusks, or crustaceans. Along with the vibrio polysaccharide, secreted proteins of the rbm gene cluster are key to the biofilm ultrastructure. Here we provide a thorough structural characterization of RbmA, a protein involved in mediating cell-cell and cell-biofilm contacts. We correlate our structural findings with initial ligand specificity screening results, NMR protein-ligand interaction analysis, and complement our results with a full biocomputational study.
authors: Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
authors: Manuel
authors: M
authors: Maestre-Reyna
authors: Wen-Jin
authors: WJ
authors: Wu
authors: Andrew H-J
authors: AH
authors: Wang
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018441:Biofilms; D008024:Ligands; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary; D013329:Structure-Activity Relationship; D014734:Vibrio cholerae / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D008024:Ligands