title: Monitoring DNA hybridization using optical microcavities.
abstract: The development of DNA analysis methods is rapidly expanding as interest in characterizing subtle variations increases in biomedicine. A promising approach is based on evanescent field sensors that monitor the hybridization process in real time. However, one challenge is discriminating between nonspecific and specific attachment. Here, we demonstrate a hybridization sensor based on an integrated toroidal optical microcavity. The surface is functionalized with ssDNA using an epoxide method, and the evanescent wave of the microresonator excites a fluorescent label on the complementary ssDNA during hybridization. Based on a temporal analysis, the different binding regimes can be identified.
authors: Rasheeda M
authors: RM
authors: Hawk
authors: Maria V
authors: MV
authors: Chistiakova
authors: Andrea M
authors: AM
authors: Armani
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017403:In Situ Hybridization / Q000295:instrumentation*; D017422:Sequence Analysis, DNA / Q000295:instrumentation*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D018511:Systems Integration
chemical_list: D004247:DNA