title: Protective effect of an egg yolk-derived immunoglobulin (IgY) against Prevotella intermedia-mediated gingivitis.
abstract: OBJECTIVE
  To investigate the effects of an egg yolk-derived immunoglobulin (IgY) specific to Prevotella intermedia in vitro and in vivo.
  
  
  RESULTS
  An IgY specific to P. intermedia was produced by immunizing hens with formaldehyde-inactivated P. intermedia and showed high titres when subjected to an ELISA. The obtained IgY inhibited the growth of P. intermedia in a dose-dependent manner at concentrations from 1 to 20 mg ml(-1) in Center for Disease Control and Prevention liquid medium. Forty rats were challenged with P. intermedia on gingivae and then randomly divided into four groups, which were syringed respectively with phosphate-buffered saline, 1 mg ml(-1) of tinidazole, 20 mg ml(-1) of nonspecific IgY and 20 mg ml(-1) of the IgY specific to P. intermedia at a dosage of 300 μl per day. Gingival index (GI), plaque index (PI), bleeding on probing (BOP), counts of white blood cell (WBC) and histopathological slide of the gums were measured after treatment for 15 days. The gingivitis rats treated with the IgY specific to P. intermedia showed significantly decreased GI, PI, BOP and WBC (P < 0·05). Gum histopathology of the treated rats demonstrated a superior protective effect of the specific IgY on P. intermedia-mediated gingivitis.
  
  
  CONCLUSIONS
  A new immunoglobulin specific to P. intermedia was developed from egg yolk. This specific IgY can dose-dependently inhibit the growth of P. intermedia and protect rats from gingivitis induced by P. intermedia.
  
  
  CONCLUSIONS
  The new IgY has potential for the treatment of P. intermedia-mediated gingivitis.
authors: Clinical Medicine of Seven-year-program, Dalian Medical University, Dalian, China.
authors: Y-Y
authors: YY
authors: Hou
authors: Y-H
authors: YH
authors: Zhen
authors: D
authors: D
authors: Wang
authors: J
authors: J
authors: Zhu
authors: D-X
authors: DX
authors: Sun
authors: X-T
authors: XT
authors: Liu
authors: H-X
authors: HX
authors: Wang
authors: Y
authors: Y
authors: Liu
authors: Y-Y
authors: YY
authors: Long
authors: X-H
authors: XH
authors: Shu
mesh_terms: D000818:Animals; D016866:Bacteroidaceae Infections / Q000628:therapy*; D002645:Chickens / Q000276:immunology; D004530:Egg Yolk / Q000276:immunology; D005260:Female; D005891:Gingivitis / Q000382:microbiology / Q000628:therapy*; D007136:Immunoglobulins / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D018713:Prevotella intermedia / Q000254:growth & development / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C016512:IgY; D007136:Immunoglobulins
keywords: Prevotella intermedia; bleeding on probing; egg yolk-derived immunoglobulin; gingival index; plaque index; rat gingivitis