title: Methylation of translation elongation factor 1A by the METTL10-like See1 methyltransferase facilitates tombusvirus replication in yeast and plants.
abstract: Replication of tombusviruses and other plus-strand RNA viruses depends on several host factors that are recruited into viral replicase complexes. Previous studies have shown that eukaryotic translation elongation factor 1A (eEF1A) is one of the resident host proteins in the highly purified tombusvirus replicase complex. In this paper, we show that methylation of eEF1A by the METTL10-like See1p methyltransferase is required for tombusvirus and unrelated nodavirus RNA replication in yeast model host. Similar to the effect of SEE1 deletion, yeast expressing only a mutant form of eEF1A lacking the 4 known lysines subjected to methylation supported reduced TBSV accumulation. We show that the half-life of several viral replication proteins is decreased in see1Δ yeast or when a mutated eEF1A was expressed as a sole source for eEF1A. Silencing of the plant ortholog of See1 methyltransferase also decreased tombusvirus RNA accumulation in Nicotiana benthamiana.
authors: Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, United States.
authors: Zhenghe
authors: Z
authors: Li
authors: Paulina Alatriste
authors: PA
authors: Gonzalez
authors: Zsuzsanna
authors: Z
authors: Sasvari
authors: Terri Goss
authors: TG
authors: Kinzy
authors: Peter D
authors: PD
authors: Nagy
mesh_terms: D054884:Host-Pathogen Interactions; D008745:Methylation; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D020648:Peptide Elongation Factor 1 / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism / Q000821:virology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000201:enzymology* / Q000378:metabolism / Q000821:virology; D017864:Tombusvirus / Q000235:genetics / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication
chemical_list: D020648:Peptide Elongation Factor 1; D010940:Plant Proteins; D029701:Saccharomyces cerevisiae Proteins; C410826:TEF1 protein, S cerevisiae; D014764:Viral Proteins; D008780:Methyltransferases; C586424:See1 protein, S cerevisiae
keywords: Host factor; Methyltransferase; Nodaviruses; Plant; Posttranslational modification; Replication; Tomato bushy stunt virus; Tombusviruses; Translation elongation factor 1A; Virus–host interaction; Yeast