title: Comparative chromatography-mass spectrometry studies on the antiretroviral drug nevirapine-analytical performance characteristics in human plasma determination.
abstract: A contrast between the analytical performance characteristics using gas chromatography-mass spectrometry (GC-MS) liquid chromatography-mass spectrometry (LC-MS) and liquid chromatography-ultraviolet (LC-UV) detection for the determination of the antiretroviral drug (ARV) nevirapine (NVP) in fortified human plasma after QuEChERS extraction has been made. Analytical performance characteristics, i.e. linearities, instrument detection limits (IDLs), limits of quantitation (LOQs), method detection limits (MDLs), % mean recoveries and the corresponding relative standard deviations (%RSDs) were estimated using techniques above. Using GC-MS, the correlation coefficients (r(2)) were ≥0.990, which were deemed acceptable linearities. The MDLs ranged between 11.1-29.8μg/L and 13.7-36.0μg/L using helium and hydrogen carrier gases respectively. The LOQs ranged between 16.5-66.7μg/L and 28.4-98.7μg/L using helium and hydrogen carrier gases respectively with a % mean recovery of 83% and %RSD of 4.6%. Using LC-MS and LC-UV, the correlation coefficients (r(2)) were ≥0.990. The MDLs were ranged between 3.14 and 47.1μg/L. The LOQs ranged between 2.85 and 90.0μg/L respectively. The MDLs using GC-MS, LC-MS and LC-UV were below the therapeutic range for NVP in human plasma is considered to be between 2300μg/L (Cmin) and 8000μg/L (Cmax). This study also demonstrated that helium can be substituted with hydrogen which is relatively cheaper and easily obtainable even by use of a generator.
authors: University of Botswana, Department of Chemistry, Faculty of Science, PB UB 00704 Gaborone, Botswana. Electronic address: kwenga.sichilongo@mopipi.ub.bw.
authors: Kwenga
authors: K
authors: Sichilongo
authors: University of Botswana, Department of Chemistry, Faculty of Science, PB UB 00704 Gaborone, Botswana.
authors: Mompati
authors: M
authors: Chinyama
authors: University of Botswana, Department of Biomedical Sciences, School of Medicine, PB UB 00713 Gaborone, Botswana.
authors: Amos
authors: A
authors: Massele
authors: University of Botswana, Department of Internal Medicine, School of Medicine, PB UB 00713 Gaborone, Botswana.
authors: Sandro
authors: S
authors: Vento
mesh_terms: D019380:Anti-HIV Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006371:Helium / Q000737:chemistry; D006801:Humans; D006859:Hydrogen / Q000737:chemistry; D057230:Limit of Detection; D019829:Nevirapine / Q000097:blood*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D019380:Anti-HIV Agents; D006371:Helium; D006859:Hydrogen; D019829:Nevirapine
keywords: GC–MS; LC–MS; LC–UV; Nevirapine; Therapeutic drug monitoring