title: Graphene oxide can induce in vitro and in vivo mutagenesis.
abstract: Graphene oxide (GO) has attracted enormous interests due to its extraordinary properties. Recent studies have confirmed the cytotoxicity of GO, we further investigate its mutagenic potential in this study. The results showed that GO interfered with DNA replication and induced mutagenesis at molecular level. GO treatments at concentrations of 10 and 100 μg/mL altered gene expression patterns at cellular level, and 101 differentially expressed genes mediated DNA-damage control, cell apoptosis, cell cycle, and metabolism. Intravenous injection of GO at 4 mg/kg for 5 consecutive days clearly induced formation of micronucleated polychromic erythrocytes in mice, and its mutagenesis potential appeared to be comparable to cyclophosphamide, a classic mutagen. In conclusion, GO can induce mutagenesis both in vitro and in vivo, thus extra consideration is required for its biomedical applications.
authors: Research Center of Basic Medical Science & School of Pharmacy, Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), Tianjin Medical University, No. 22 Qixiangtai Road, Heping District, Tianjin 300070, People's Republic of China.
authors: Yuanyuan
authors: Y
authors: Liu
authors: Yi
authors: Y
authors: Luo
authors: Jing
authors: J
authors: Wu
authors: Yinsong
authors: Y
authors: Wang
authors: Xiaoying
authors: X
authors: Yang
authors: Rui
authors: R
authors: Yang
authors: Baiqi
authors: B
authors: Wang
authors: Jinrong
authors: J
authors: Yang
authors: Ning
authors: N
authors: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016000:Cluster Analysis; D004249:DNA Damage / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D006108:Graphite / Q000737:chemistry / Q000633:toxicity*; D006801:Humans; D008297:Male; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced; D016296:Mutagenesis / Q000187:drug effects*; D010087:Oxides / Q000737:chemistry; D051744:Protein Kinase C-epsilon / Q000235:genetics
chemical_list: D010087:Oxides; D006108:Graphite; D051744:Protein Kinase C-epsilon