title: A comparison of microbial characteristics between the thermophilic and mesophilic anaerobic digesters exposed to elevated food waste loadings.
abstract: Thermophilic and mesophilic anaerobic digestion reactors (TR and MR) using food waste as substrate were compared with emphasis on microbial responses to increasing organic loading rate (OLR). At OLR ranging from 1.0 to 2.5 g VS L(-1) d(-1), MR exhibited more stable performance compared to TR in terms of methane yield. Amplicons pyrosequencing results revealed the distinct microbial dynamics in the two reactors. Primarily, MR had greater richness and evenness of bacteria species. With OLR elevated, larger shifts of bacterial phylogeny were observed in MR; Methanosaeta dominated in archaeal community in MR while Methanothermobacter and Methanoculleus were favored in TR. The high functional redundancy in bacterial community integrated with acetoclastic methanogenesis in MR resulted in its better performance; whereas delicate interactions between hydrogen-producer and hydrogenotrophic methanogens in TR were much more prone to disruption. These results are conductive to understanding the microbial mechanisms of low methane yield during food waste anaerobic digestion.
authors: College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
authors: Xiaohui
authors: X
authors: Guo
authors: College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
authors: Cheng
authors: C
authors: Wang
authors: College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
authors: Faqian
authors: F
authors: Sun
authors: College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
authors: Weijing
authors: W
authors: Zhu
authors: College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: weixiang@zju.edu.cn.
authors: Weixiang
authors: W
authors: Wu
mesh_terms: D000693:Anaerobiosis; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D056804:Biofuels / Q000382:microbiology; D019149:Bioreactors / Q000382:microbiology*; D005502:Food; D015723:Gene Library; D008697:Methane / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature; D014866:Waste Products
chemical_list: D056804:Biofuels; D012336:RNA, Ribosomal, 16S; D014866:Waste Products; D008697:Methane
keywords: Anaerobic digestion; Food waste; Microbial community; Pyrosequencing; Thermophilic and mesophilic