title: Outbreak of H3N2 influenza at a US military base in Djibouti during the H1N1 pandemic of 2009.
abstract: BACKGROUND
  Influenza pandemics have significant operational impact on deployed military personnel working in areas throughout the world. The US Department of Defense global influenza-like illness (ILI) surveillance network serves an important role in establishing baseline trends and can be leveraged to respond to outbreaks of respiratory illness.
  
  
  OBJECTIVE
  We identified and characterized an operationally unique outbreak of H3N2 influenza at Camp Lemonnier, Djibouti occurring simultaneously with the H1N1 pandemic of 2009 [A(H1N1)pdm09].
  
  
  METHODS
  Enhanced surveillance for ILI was conducted at Camp Lemonnier in response to local reports of a possible outbreak during the A(H1N1)pdm09 pandemic. Samples were collected from consenting patients presenting with ILI (utilizing a modified case definition) and who completed a case report form. Samples were cultured and analyzed using standard real-time reverse transcriptase PCR (rt-RT-PCR) methodology and sequenced genetic material was phylogenetically compared to other published strains.
  
  
  RESULTS
  rt-RT-PCR and DNA sequencing revealed that 25 (78%) of the 32 clinical samples collected were seasonal H3N2 and only 2 (6%) were A(H1N1)pdm09 influenza. The highest incidence of H3N2 occurred during the month of May and 80% of these were active duty military personnel. Phylogenetic analysis revealed that sequenced H3N2 strains were genetically similar to 2009 strains from the United States of America, Australia, and South east Asia.
  
  
  CONCLUSIONS
  This outbreak highlights challenges in the investigation of influenza among deployed military populations and corroborates the public health importance of maintaining surveillance systems for ILI that can be enhanced locally when needed.
authors: United States Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt.
authors: Michael T
authors: MT
authors: Cosby
authors: Guillermo
authors: G
authors: Pimentel
authors: Remington L
authors: RL
authors: Nevin
authors: Salwa
authors: S
authors: Fouad Ahmed
authors: John D
authors: JD
authors: Klena
authors: Ehab
authors: E
authors: Amir
authors: Mary
authors: M
authors: Younan
authors: Robert
authors: R
authors: Browning
authors: Peter J
authors: PJ
authors: Sebeny
mesh_terms: D004196:Disease Outbreaks; D015730:Djibouti / Q000453:epidemiology; D005260:Female; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype; D053122:Influenza A Virus, H3N2 Subtype; D007251:Influenza, Human / Q000453:epidemiology* / Q000235:genetics / Q000378:metabolism; D008297:Male; D008889:Military Personnel; D058873:Pandemics; D012367:RNA, Viral / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014481:United States
chemical_list: D012367:RNA, Viral