title: An insight into the ribonucleolytic and antiangiogenic activity of buffalo lactoferrin.
abstract: Lactoferrin (LF) has several biological effects ranging from ribonuclease activity to antiangiogenic activity. It thus serves as a potential target protein for studies related to ribonucleolytic activity in association with its antiangiogenic activity. We have isolated buffalo LF and checked the ribonucleolytic activity via an agarose gel-based assay and precipitation assay. The ribonucleolytic activity of LF is lower compared to RNase A and the pH profile is a bell-shaped curve, with a pK1 value of 5.43 and pK2 of 7.65. The ribonuclease inhibitor that inhibits many ribonuclease-type proteins by forming a tight complex is unable to inhibit the ribonucleolytic property of LF. Fe(III) behaves as a noncompetitive inhibitor for the ribonucleolytic activity of protein. The superoxide-scavenging activity of the protein has also been measured. Histidine modification by diethylpyrocarbonate was monitored by UV-Vis spectroscopy at pH 7 and pH 8 and the effect towards the ribonucleolytic activity was determined. The antiangiogenic property of LF was investigated by the chorioallantoic membrane assay. Finally, the possible active site was analyzed via docking studies and correlated with the experimental study.
authors: a Department of Chemistry, Indian Institute of Technology , Kharagpur 721302 , India.
authors: Debi Ranjan
authors: DR
authors: Tripathy
authors: Nitin K
authors: NK
authors: Pandey
authors: Amit Kumar
authors: AK
authors: Dinda
authors: Sudeshna
authors: S
authors: Ghosh
authors: Atanu
authors: A
authors: Singha Roy
authors: Swagata
authors: S
authors: Dasgupta
mesh_terms: D000465:Algorithms; D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002020:Buffaloes; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007781:Lactoferrin / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D008958:Models, Molecular; D008970:Molecular Weight; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012313:RNA / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism*; D013481:Superoxides / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D020533:Angiogenesis Inhibitors; D016166:Free Radical Scavengers; D013481:Superoxides; D012313:RNA; D012260:Ribonucleases; D007781:Lactoferrin
keywords: anti angiogenic property; buffalo lactoferrin; ribonuclease inhibitor; ribonucleolytic property; superoxide-scavenging property