title: Serological and molecular characteristics of the first Korean case of Echinococcus multilocularis.
abstract: In December 2011, we reported an autochthonous case of Echinococcus multilocularis infection in a 42-year-old woman in Korea. The diagnosis was based on histopathological findings of the surgically resected liver cyst. In the present study, we evaluated the serological and molecular characteristics of this Korean E. multilocularis case. The patient's serum strongly reacted with affinity-purified native Em18 and recombinant Em18 antigens (specific for E. multilocularis) but negative for recombinant antigen B8/1 (reactive for Echinococcus granulosus). In immunoaffinity chromatography, the serum also strongly reacted with E. multilocularis and only weakly positive for E. granulosus. We determined the whole nucleotide sequence of cox1 (1,608 bp) using the paraffin-embedded cystic tissue which was compared with E. multilocularis isolates from China, Japan, Kazakhstan, Austria, France, and Slovakia. The Korean case showed 99.8-99.9% similarity with isolates from Asia (the highest similarity with an isolate from Sichuan, China), whereas the similarity with European isolates ranged from 99.5 to 99.6%.
authors: Department of Pathology, Dong-A University College of Medicine, Busan 602-714, Korea.
authors: Jin-Sook
authors: JS
authors: Jeong
authors: Sang-Young
authors: SY
authors: Han
authors: Young-Hoon
authors: YH
authors: Kim
authors: Yasuhito
authors: Y
authors: Sako
authors: Tetsuya
authors: T
authors: Yanagida
authors: Akira
authors: A
authors: Ito
authors: Jong-Yil
authors: JY
authors: Chai
mesh_terms: D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001483:Base Sequence; D004444:Echinococcosis, Hepatic / Q000276:immunology* / Q000469:parasitology; D004445:Echinococcosis, Pulmonary / Q000175:diagnosis / Q000235:genetics / Q000276:immunology; D048209:Echinococcus granulosus / Q000235:genetics / Q000276:immunology; D048210:Echinococcus multilocularis / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D003576:Electron Transport Complex IV / Q000235:genetics; D005260:Female; D006801:Humans; D008928:Mitochondria / Q000235:genetics; D008969:Molecular Sequence Data; D056910:Republic of Korea; D017422:Sequence Analysis, DNA
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; C117889:Em18 antigen; D003576:Electron Transport Complex IV
keywords: Echinococcus multilocularis; South Korea; alveolar echinococcosis; cox1; immunodiagnosis