title: [Math1 gene therapy for kanamycin and furosemide-induced deaf guinea pigs].
abstract: OBJECTIVE
  To observe the morphology and function changes of cochlear hair cells before and after math1 gene injection into the cochlea of deaf guinea pigs which were induced by kanamycin and furosemide. To explore the feasibility of Math1 gene for medicine-induced deafness therapy.
  
  
  METHODS
  Kanamycin (500 mg/kg) and furosemide (50 mg/kg) were given to the healthy adult guinea pigs intramuscularly and intravenously to establish the deafness model. The guinea pigs whose auditory brainstem response (ABR) threshold > 95 dB SPL were randomly divided into five groups. Blank control group (without any treatment, n = 3), operation control group (right ear scala tympani operation, n = 3), artificial perilymph group (right ear scala tympani injection artificial perilymph, n = 3), virus vector group [right ear scala tympani injection adenovirus which carrying enhanced green fluorescent protein (EGFP) gene (Ad. EGFP) , n = 4], Math1 gene therapy group [right ear scala tympani injection adenovirus which carrying Math1 and EGFP gene (Ad. Math1-EGFP), n = 6]. Each animal received ABR test before and after injection. The cochlear tissue was observed by scanning electronic microscopy.
  
  
  RESULTS
  The ABR thresholds of tone burst( 4, 8, 16, 20 kHz ) were not statistically significant in different groups (P > 0.05). The number of hair cells increased in some of severe deaf guinea pigs after the injection of Ad. Math1-EGFP gene. However, there was no obvious difference with morphology and numbers of cochlea hair cells in other groups.
  
  
  CONCLUSIONS
  The injection of Math1 gene to cochlea can regenerate or repair the hair cells of medicine-induced deaf guinea pigs, but there was no improvement on the hearing loss.
authors: Department of Otorhinolaryngology, Yantai Hill Hospital, Yantai, China.
authors: Xian-Fen
authors: XF
authors: Zhang
authors: Shi-Ming
authors: SM
authors: Yang
authors: Dong-Yi
authors: DY
authors: Han
authors: Wei-Wei
authors: WW
authors: Guo
authors: Jian-He
authors: JH
authors: Sun
authors: Ju
authors: J
authors: Gao
authors: Dong-Xiu
authors: DX
authors: Sun
authors: Ai-Ling
authors: AL
authors: Sun
authors: Zhen
authors: Z
authors: Li
authors: Xiao-Na
authors: XN
authors: Wei
mesh_terms: D000256:Adenoviridae; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics*; D003051:Cochlea; D003638:Deafness; D007758:Ear, Inner; D016057:Evoked Potentials, Auditory, Brain Stem; D005665:Furosemide / Q000633:toxicity*; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D006168:Guinea Pigs; D006198:Hair Cells, Auditory; D034381:Hearing Loss / Q000139:chemically induced* / Q000235:genetics; D007612:Kanamycin / Q000633:toxicity*; D010498:Perilymph
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D005665:Furosemide; D049452:Green Fluorescent Proteins; D007612:Kanamycin; C485184:enhanced green fluorescent protein