title: Bovine and murine tissue expression of insulin like growth factor-I.
abstract: The genomic architecture and expression of the Igf-1 gene are complex yielding multiple IGF-I transcript isoforms with putative functional contributions to growth and metabolism. Using RNA-seq on different tissues, physiological states, and species, the breadth of transcripts expressed was determined. Tissues from pre- and post-pubertal heifers and mature mice were collected and the transcript isoforms characterized. Three different IGF-I isoforms were detected in heifers with Class 1 transcripts most abundantly expressed. The pituitary reduced IGF-I expression post-pubertally whereas the uterus increased expression. Murine IGF-I transcript expression was more diverse utilizing multiple exons, start sites, and 3'UTRs. The RNA-seq methodology to characterize expression profiles permits assessment of the transcript isoforms yielding insight into functional roles of each transcript.
authors: Department of Animal Science, University of California, Davis, CA, USA. Electronic address: amoberbauer@ucdavis.edu.
authors: A M
authors: AM
authors: Oberbauer
authors: Department of Animal Science, University of California, Davis, CA, USA. Electronic address: jmbelanger@ucddavis.edu.
authors: J M
authors: JM
authors: Belanger
authors: Department of Animal Science, University of California, Davis, CA, USA. Electronic address: gonzalo.rincon@zoetis.com.
authors: G
authors: G
authors: Rincon
authors: Department of Animal Science, University of California, Davis, CA, USA. Electronic address: acanovas@ucdavis.edu.
authors: A
authors: A
authors: Cánovas
authors: Department of Animal Science, University of California, Davis, CA, USA. Electronic address: adislas@ucdavis.edu.
authors: A
authors: A
authors: Islas-Trejo
authors: Department of Animal Science, University of California, Davis, CA, USA. Electronic address: rodrigo.gularte@ulg.ac.be.
authors: R
authors: R
authors: Gularte-Mérida
authors: Department of Animal Sciences, Colorado State University, Fort Collins, CO, USA. Electronic address: milt.Thomas@colostate.edu.
authors: M G
authors: MG
authors: Thomas
authors: Department of Animal Science, University of California, Davis, CA, USA. Electronic address: jfmedrano@ucdavis.edu.
authors: J F
authors: JF
authors: Medrano
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D002417:Cattle; D005260:Female; D023061:Gene Order; D007334:Insulin-Like Growth Factor I / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D009928:Organ Specificity / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012741:Sexual Maturation / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I
keywords: Alternative splicing; Heifer; IGF-I; Mouse; Prepubertal; RNA quality indicator; RNA-seq; RPKM; RQI; Splice variant; insulin like growth factor I; reads per kilobase per million mapped reads; ribonucleic acid sequencing