title: An angular fluidic channel for prism-free surface-plasmon-assisted fluorescence capturing.
abstract: Surface plasmon excitation provides stronger enhancement of the fluorescence intensity and better sensitivity than other sensing approaches but requires optimal positioning of a prism to ensure optimum output of the incident light. Here we describe a simple, highly sensitive optical sensing system combining surface plasmon excitation and fluorescence to address this limitation. V-shaped fluidic channels are employed to mimic the functions of a prism, sensing plate, and flow channel in a single setup. Superior performance is demonstrated for different biomolecular recognition reactions on a self-assembled monolayer, and the sensitivity reaches 100 fM for biotin-streptavidin interactions. Using an antibody as a probe, we demonstrate the detection of intact influenza viruses at 0.2 HA units ml⁻¹ levels. The convenient sensing system developed here has the advantages of being prism-free and requiring less sample (1-2 μl), making this platform suitable for use in situations requiring low sample volumes.
authors: 1] Flexible Electronics Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan [2].
authors: Ken-ichi
authors: K
authors: Nomura
authors: 1] Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan [2].
authors: Subash C B
authors: SC
authors: Gopinath
authors: Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors: Thangavel
authors: T
authors: Lakshmipriya
authors: Flexible Electronics Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors: Nobuko
authors: N
authors: Fukuda
authors: Nano-electronics Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan.
authors: Xiaomin
authors: X
authors: Wang
authors: Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors: Makoto
authors: M
authors: Fujimaki
mesh_terms: D000906:Antibodies / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D005453:Fluorescence; D006046:Gold / Q000737:chemistry; D053122:Influenza A Virus, H3N2 Subtype / Q000737:chemistry / Q000302:isolation & purification*; D019809:Streptavidin / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
chemical_list: D000906:Antibodies; D001710:Biotin; D006046:Gold; D019809:Streptavidin