title: Dichamanetin inhibits cancer cell growth by affecting ROS-related signaling components through mitochondrial-mediated apoptosis.
abstract: OBJECTIVE
  Dichamanetin is a C-benzylated flavanone isolated as a major secondary metabolite from Piper sarmentosum, a plant used as a spice in Southeast Asia. This study aimed to investigate the path through which dichamanetin exerts its antiproliferative effect.
  
  
  METHODS
  The study of several signaling cellular components, namely, reactive oxygen species (ROS) levels, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) transcription factor, mitochondrial membrane potential, DNA binding, poly ADP-ribose polymerase (PARP1) inhibition and proteasome inhibition was performed using an enzyme-linked immunosorbent (ELISA) assay, cell sorting, and western blot.
  
  
  RESULTS
  Dichamanetin significantly reduced the cell viability of various types of human cancer cells (HT-29 colon, DU145 prostate, and MDA-MB-231 breast cancer) in a concentration- and time-dependent manner and induced G1 arrest of the cell cycle. It was also demonstrated that the selective cytotoxic effect of dichamanetin in cancer cells is mediated by the induction of oxidative stress.
  
  
  CONCLUSIONS
  Our findings suggest that dichamanetin isolated from an edible herb has cancer chemotherapeutic potential.
authors: Division of Pharmacy Practice and Administration, Division of Medicinal Chemistry and Pharmacognosy, College of Phamacy, The OhioState University, 500 West 12th Avenue, Columbus, OH 43210-1291, U.S.A. carcache-de-blan.1@osu.edu.
authors: Yeonjoong
authors: Y
authors: Yong
authors: Susan
authors: S
authors: Matthew
authors: Jennifer
authors: J
authors: Wittwer
authors: Li
authors: L
authors: Pan
authors: Qi
authors: Q
authors: Shen
authors: A Douglas
authors: AD
authors: Kinghorn
authors: Steven M
authors: SM
authors: Swanson
authors: Esperanza J Carcache
authors: EJ
authors: DE Blanco
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D044950:Flavanones / Q000494:pharmacology*; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000502:physiology*; D016328:NF-kappa B / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D044950:Flavanones; D016328:NF-kappa B; D017382:Reactive Oxygen Species; C512547:dichamanetin
keywords: DNA binding; NF-κB inhibition; PARP-1; Piper sarmentosum; Piperaceae; ROS; cytotoxicity; dichamanetin; mitochondrial membrane potential