title: Plasmodium falciparum CENH3 is able to functionally complement Cse4p and its, C-terminus is essential for centromere function.
abstract: The Plasmodium falciparum centromeric histone variant PfCENH3 has been shown to occupy a 4-4.5 kb region on each chromosome, but the experimental demonstration of its structure-function relationship remains unexplored. By functional complementation assays, we report that the C-terminus, specifically the CATD region within the HFD of PfCENH3 is essential in centromere function. Our studies also indicate that the PfCENH3 specific LLAL residues of the CATD region are required for centromere targeting and chromosome segregation. Histone H3 of P. falciparum is not found to complement Cse4p (the yeast homologue of CENH3). We also report the identification of PfCENP-C, another component of the inner kinetochore protein complex and its association with PfCENH3. These studies thus delineate the structural determinants of PfCENH3.
authors: Molecular Parasitology Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bangalore 560064, India.
authors: Garima
authors: G
authors: Verma
authors: Molecular Parasitology Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bangalore 560064, India. Electronic address: surolia@jncasr.ac.in.
authors: Namita
authors: N
authors: Surolia
mesh_terms: D000595:Amino Acid Sequence; D001324:Autoantigens / Q000737:chemistry / Q000235:genetics*; D002503:Centromere / Q000235:genetics* / Q000378:metabolism; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000235:genetics*; D005816:Genetic Complementation Test; D006657:Histones / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics* / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs; D017433:Protein Structure, Secondary; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D001324:Autoantigens; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D029701:Saccharomyces cerevisiae Proteins
keywords: CATD; Cse4p; Functional complementation; PfCENH3; PfCENP-C; Plasmodium falciparum