title: Studies of metabolite-protein interactions: a review.
abstract: The study of metabolomics can provide valuable information about biochemical pathways and processes at the molecular level. There have been many reports that have examined the structure, identity and concentrations of metabolites in biological systems. However, the binding of metabolites with proteins is also of growing interest. This review examines past reports that have looked at the binding of various types of metabolites with proteins. An overview of the techniques that have been used to characterize and study metabolite-protein binding is first provided. This is followed by examples of studies that have investigated the binding of hormones, fatty acids, drugs or other xenobiotics, and their metabolites with transport proteins and receptors. These examples include reports that have considered the structure of the resulting solute-protein complexes, the nature of the binding sites, the strength of these interactions, the variations in these interactions with solute structure, and the kinetics of these reactions. The possible effects of metabolic diseases on these processes, including the impact of alterations in the structure and function of proteins, are also considered.
authors: Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
authors: Ryan
authors: R
authors: Matsuda
authors: Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
authors: Cong
authors: C
authors: Bi
authors: Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
authors: Jeanethe
authors: J
authors: Anguizola
authors: Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
authors: Matthew
authors: M
authors: Sobansky
authors: Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
authors: Elliott
authors: E
authors: Rodriguez
authors: Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
authors: John
authors: J
authors: Vargas Badilla
authors: Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
authors: Xiwei
authors: X
authors: Zheng
authors: Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
authors: Benjamin
authors: B
authors: Hage
authors: Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA. Electronic address: dhage1@unl.edu.
authors: David S
authors: DS
authors: Hage
mesh_terms: D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D055442:Metabolome; D055432:Metabolomics; D008958:Models, Molecular; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D004364:Pharmaceutical Preparations; D011506:Proteins
keywords: Drug–protein interactions; Fatty acid–protein interactions; Hormone–protein interactions; Metabolomics; Protein modification; Xenobiotic–protein interactions