title: Action spectra of photosystems II and I and quantum yield of photosynthesis in leaves in State 1.
abstract: The spectral global quantum yield (YII, electrons/photons absorbed) of photosystem II (PSII) was measured in sunflower leaves in State 1 using monochromatic light. The global quantum yield of PSI (YI) was measured using low-intensity monochromatic light flashes and the associated transmittance change at 810nm. The 810-nm signal change was calibrated based on the number of electrons generated by PSII during the flash (4·O2 evolution) which arrived at the PSI donor side after a delay of 2ms. The intrinsic quantum yield of PSI (yI, electrons per photon absorbed by PSI) was measured at 712nm, where photon absorption by PSII was small. The results were used to resolve the individual spectra of the excitation partitioning coefficients between PSI (aI) and PSII (aII) in leaves. For comparison, pigment-protein complexes for PSII and PSI were isolated, separated by sucrose density ultracentrifugation, and their optical density was measured. A good correlation was obtained for the spectral excitation partitioning coefficients measured by these different methods. The intrinsic yield of PSI was high (yI=0.88), but it absorbed only about 1/3 of quanta; consequently, about 2/3 of quanta were absorbed by PSII, but processed with the low intrinsic yield yII=0.63. In PSII, the quantum yield of charge separation was 0.89 as detected by variable fluorescence Fv/Fm, but 29% of separated charges recombined (Laisk A, Eichelmann H and Oja V, Photosynth. Res. 113, 145-155). At wavelengths less than 580nm about 30% of excitation is absorbed by pigments poorly connected to either photosystem, most likely carotenoids bound in pigment-protein complexes.
authors: Tartu Ülikooli Molekulaar- ja Rakubioloogia Instituut, Riia tn. 23, Tartu 51010, Estonia. Electronic address: alaisk@ut.ee.
authors: Agu
authors: A
authors: Laisk
authors: Tartu Ülikooli Molekulaar- ja Rakubioloogia Instituut, Riia tn. 23, Tartu 51010, Estonia.
authors: Vello
authors: V
authors: Oja
authors: Tartu Ülikooli Molekulaar- ja Rakubioloogia Instituut, Riia tn. 23, Tartu 51010, Estonia.
authors: Hillar
authors: H
authors: Eichelmann
authors: Università di Verona, Dipartimento di Biotecnologie, Strada Le Grazie, 15 37135 Verona, Italy.
authors: Luca
authors: L
authors: Dall'Osto
mesh_terms: D004579:Electron Transport; D004583:Electrons; D006368:Helianthus / Q000378:metabolism*; D007259:Infrared Rays; D010100:Oxygen / Q000378:metabolism; D017785:Photons; D010788:Photosynthesis; D045331:Photosystem I Protein Complex / Q000378:metabolism*; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism*; D011789:Quantum Theory; D013057:Spectrum Analysis; D013997:Time Factors
chemical_list: D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D010100:Oxygen
keywords: A/D; Car; Chl; ETR; Electron transport; Excitation partitioning; FRL; GL; LED; LHCI, LHCII; Leaves; P700; PAD, PFD; PC; PQ(H(2)); PSI, PSII; Photosystems; Quantum yield; STF; WOC; Y(II), Y(I); analog-to-digital converter; carotenoids; chlorophyll; donor pigment of PSI; electron transport rate; far-red light; global quantum yield of the photosystem with respect to all quanta absorbed by the leaf; green light (540nm); intrinsic quantum yield of the photosystem with respect to quanta absorbed by the particular photosystem; light-emitting diode; light-harvesting complexes of PSI and PSII; photon flux density, absorbed and incident; photosystems I and II; plastocyanin; plastoquinone (reduced); single turnover flash; water-oxidizing complex; y(II), y(I)