title: Expression of the rodent-specific alternative splice variant of tryptophanyl-tRNA synthetase in murine tissues and cells.
abstract: Tryptophanyl-tRNA synthetase (TrpRS) catalyzes the aminoacylation of tRNA(Trp). mRNA of a rodent-specific alternative splice variant of TrpRS (SV-TrpRS), which results in the inclusion of an additional hexapeptide at the C-terminus of full-length TrpRS (FL-TrpRS), has been identified in murine embryonic stem (ES) cells. In the present study, we evaluated the expression of mouse TrpRS mRNA by real-time reverse transcription PCR. We show that SV-TrpRS and FL-TrpRS mRNAs are highly expressed in murine ES cells, embryo, spleen, lung, liver and uterus, and that the relative expression of SV-TrpRS compared to FL-TrpRS is significantly less in the brain. Moreover, we found that interferon-γ increases the expression of TrpRS in a mouse cell line. These results provide the first evidence for tissue-specific expression and alternative splicing of mouse TrpRS.
authors: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.
authors: Miki
authors: M
authors: Miyanokoshi
authors: Tomoaki
authors: T
authors: Tanaka
authors: Miho
authors: M
authors: Tamai
authors: Yoh-ichi
authors: Y
authors: Tagawa
authors: Keisuke
authors: K
authors: Wakasugi
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D007371:Interferon-gamma / Q000494:pharmacology; D051379:Mice; D009928:Organ Specificity; D059368:RNA Isoforms; D012333:RNA, Messenger / Q000235:genetics; D013045:Species Specificity; D014369:Tryptophan-tRNA Ligase / Q000235:genetics*
chemical_list: D059368:RNA Isoforms; D012333:RNA, Messenger; D007371:Interferon-gamma; D014369:Tryptophan-tRNA Ligase