title: Binding interactions between long noncoding RNA HOTAIR and PRC2 proteins.
abstract: Long noncoding RNAs (lncRNAs) play a key role in the epigenetic regulation of cells. Many of these lncRNAs function by interacting with histone repressive proteins of the Polycomb group (PcG) family, recruiting them to gene loci to facilitate silencing. Although there are now many RNAs known to interact with the PRC2 complex, little is known about the details of the molecular interactions. Here, we show that the PcG protein heterodimer EZH2-EED is necessary and sufficient for binding to the lncRNA HOTAIR. We also show that protein recognition occurs within a folded 89-mer domain of HOTAIR. This 89-mer represents a minimal binding motif, as further deletion of nucleotides results in substantial loss of affinity for PRC2. These findings provide molecular insights into an important system involved in epigenetic regulation.
authors: Department of Chemistry, University of Cambridge , Lensfield Road, Cambridge, CB2 1EW, United Kingdom.
authors: Liang
authors: L
authors: Wu
authors: Pierre
authors: P
authors: Murat
authors: Dijana
authors: D
authors: Matak-Vinkovic
authors: Adele
authors: A
authors: Murrell
authors: Shankar
authors: S
authors: Balasubramanian
mesh_terms: D019281:Dimerization; D000071221:Enhancer of Zeste Homolog 2 Protein; D006801:Humans; D007700:Kinetics; D009690:Nucleic Acid Conformation; D063151:Polycomb Repressive Complex 2 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D062085:RNA, Long Noncoding / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: C491488:EED protein, human; C561566:HOTAIR long untranslated RNA, human; D062085:RNA, Long Noncoding; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein; D063151:Polycomb Repressive Complex 2