title: The evolutionary history and diverse physiological roles of the grapevine calcium-dependent protein kinase gene family.
abstract: Calcium-dependent protein kinases (CDPKs) are molecular switches that bind Ca(2+), ATP, and protein substrates, acting as sensor relays and responders that convert Ca(2+) signals, created by developmental processes and environmental stresses, into phosphorylation events. The precise functions of the CDPKs in grapevine (Vitis vinifera) are largely unknown. We therefore investigated the phylogenetic relationships and expression profiles of the 17 CDPK genes identified in the 12x grapevine genome sequence, resolving them into four subfamilies based on phylogenetic tree topology and gene structures. The origins of the CDPKs during grapevine evolution were characterized, involving 13 expansion events. Transcriptomic analysis using 54 tissues and developmental stages revealed three types of CDPK gene expression profiles: constitutive (housekeeping CDPKs), partitioned functions, and prevalent in pollen/stamen. We identified two duplicated CDPK genes that had evolved from housekeeping to pollen-prevalent functions and whose origin correlated with that of seed plants, suggesting neofunctionalization with an important role in pollen development and also potential value in the breeding of seedless varieties. We also found that CDPKs were involved in three abiotic stress signaling pathways and could therefore be used to investigate the crosstalk between stress responses.
authors: Fruit Crop Systems Biology Laboratory, College of Horticulture, Nanjing Agricultural University, Nanjing, China.
authors: Fei
authors: F
authors: Chen
authors: Marianna
authors: M
authors: Fasoli
authors: Giovanni Battista
authors: GB
authors: Tornielli
authors: Silvia
authors: S
authors: Dal Santo
authors: Mario
authors: M
authors: Pezzotti
authors: Liangsheng
authors: L
authors: Zhang
authors: Bin
authors: B
authors: Cai
authors: Zong-Ming
authors: ZM
authors: Cheng
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D000255:Adenosine Triphosphate / Q000378:metabolism; D005075:Biological Evolution; D002118:Calcium / Q000378:metabolism; D020440:Gene Duplication; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D020043:Genes, Essential; D018745:Genome, Plant; D007527:Isoenzymes / Q000145:classification / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D005810:Multigene Family; D010766:Phosphorylation; D010802:Phylogeny; D010940:Plant Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D011058:Pollen / Q000201:enzymology / Q000235:genetics*; D011494:Protein Kinases / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D013312:Stress, Physiological / Q000235:genetics; D027843:Vitis / Q000201:enzymology / Q000235:genetics*; D017871:Calcium-Calmodulin-Dependent Protein Kinases
chemical_list: D000255:Adenosine Triphosphate; D002118:Calcium; D007527:Isoenzymes; D010940:Plant Proteins; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases