title: Regeneration of whole plants from callus culture of diverse genetic lines of Pisum sativum L.
abstract: Sixteen genetic lines of peas were screened for their ability to regenerate whole plants from callus cultures. Epicotyl sections from germinating seeds were placed on callus-inducing medium; the resulting callus was subcultured monthly and was tested every other month for its regeneration ability. Six lines were found that would regenerate after 2 months' growth as callus. Four of these continued to regenerate after 4 months and, of these, two after 6 months. The cultivars "Frosty" and "Alaska" were among the lines that would not regenerate at all.
authors: Department of Crop and Soil Sciences, Michigan State University, 48824, East Lansing, MI, USA.
authors: R L
authors: RL
authors: Malmberg