title: Expression of calcitonin gene-related peptide in rat pulp and periodontal tissues by indirect immunofluorescence method.
abstract: The aim of this study was to investigate the expression of nerve fibers immunoreactive to calcitonin gene-related peptide (CGRP) in pulp and periodontal tissues of rats. Male Sprague-Dawley rats, aged 6 weeks, were sacrificed, and the jaws were excised, demineralized, and processed for indirect immunofluorescence staining. A considerably higher density of nerve fibers immunoreactive to CGRP was found in the dental pulp and gingiva than in periodontal ligament. The majority of pulpal CGRP immunopositive fibers that were located followed blood vessels parallel to the long axis of the root. A subodontoblastic network of fibers IR to CGRP was found in the coronal pulp in rat molars. In the periodontium, CGRP immunopositive fibers were mainly located in the periapical area and close to the alveolar bone. Gingiva was also well supplied with CGRP-IR nerves.
authors: Department of Stomatology, The 101 Military Hospital , Wuxi, China .
authors: Yingming
authors: Y
authors: Sun
authors: Rui
authors: R
authors: Tao
authors: Mengjie
authors: M
authors: Zhang
authors: Xue
authors: X
authors: Cao
authors: Hong
authors: H
authors: Wang
authors: Lufeng
authors: L
authors: Xue
authors: Meng
authors: M
authors: Wu
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D003782:Dental Pulp / Q000294:innervation / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D008297:Male; D008856:Microscopy, Fluorescence; D009412:Nerve Fibers / Q000378:metabolism; D009928:Organ Specificity; D010519:Periodontium / Q000294:innervation / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015740:Calcitonin Gene-Related Peptide