title: Efficient expression and purification of Fc-fragment-binding domain and its application to immunoglobulin G purification.
authors: School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, Jiangsu, China.
authors: Xing Zhen
authors: XZ
authors: Lao
authors: Ya Li
authors: YL
authors: Zhou
authors: Heng
authors: H
authors: Zheng
mesh_terms: D001483:Base Sequence; D001666:Binding Sites, Antibody; D001671:Biochemistry / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D007141:Immunoglobulin Fc Fragments / Q000235:genetics / Q000378:metabolism*; D007074:Immunoglobulin G / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D008969:Molecular Sequence Data; D010957:Plasmids; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification*; D013205:Staphylococcal Protein A / Q000235:genetics / Q000378:metabolism
chemical_list: D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D008024:Ligands; D011993:Recombinant Fusion Proteins; D013205:Staphylococcal Protein A