title: Overexpression of Bmi-1 induces the malignant transformation of gastric epithelial cells in vitro.
abstract: Oncogene Bmi-1 (B-cell-specific Moloney murine leukemia virus integration site 1) has attracted much attention for its involvement in the initiation of a variety of tumors. Our previous study showed that Bmi-1 was highly expressed in gastric cancer and correlated with patient prognosis. However, whether aberrant Bmi-1 expression was critical for the transformation of gastric epithelial cells remains unknown. In this study, we stably expressed Bmi-1 in a human gastric epithelial immortalized cell line, GES-1. The overexpression of Bmi-1 promoted cell growth and proliferation, inhibited apoptosis, enhanced clone formation capability, possessed the characteristics of anchorage-independent growth, and increased migration and invasion abilities. Therefore, our findings demonstrated that ectopic expression of Bmi-1 played an important role in the malignant transformation of gastric epithelial cells.
authors: Department of Gastroenterology, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou, China.
authors: Yinting
authors: Y
authors: Chen
authors: Guoda
authors: G
authors: Lian
authors: Qiubo
authors: Q
authors: Zhang
authors: Linjuan
authors: L
authors: Zeng
authors: Chenchen
authors: C
authors: Qian
authors: Shaojie
authors: S
authors: Chen
authors: Kaihong
authors: K
authors: Huang
mesh_terms: D000818:Animals; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D005260:Female; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D063150:Polycomb Repressive Complex 1 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D011379:Prognosis; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*
chemical_list: C069077:BMI1 protein, human; D063150:Polycomb Repressive Complex 1