title: Genetic diversity in human malarial parasites of Khyber Agency Pakistan.
abstract: Malaria is wide spread in poor world and its burden has been assessed by the enumeration of malarial parasites in blood of patients. This study was designed to find a relationship between social structure, and spread of malaria in Khyber agency. The average parasite density was 2050 parasite/μl in Khyber Agency. Due to economic and social setup most of the people have habit of sleeping in open air thus playing role in high malaria prevalence and Plasmodium vivax remains the prevalent species. Genetic study performed on 110 Blood samples showed less genetic diversity for both Plasmodium vivax and Plasmodium falciparum. Eight alleles were distinguished both for Pvmsp 3α and Pvmsp 3β in total of 20 and 39 amplified samples of P. vivax respectively. Out of 17 samples amplified for P. falciparum 11 showed genotype K1 and 10 for MAD at Pfmsp-1 while 14 alleles were identified for 3D7/1C and two for FC27 of corresponding families of Pfmsp-2 gene. This shows that Plasmodium parasites are not genetically diverse in Khyber agency.
authors: Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark, lukh@sund.ku.dk.
authors: Lubna
authors: L
authors: Khatoon
authors: Muhammad Ishtiaq
authors: MI
authors: Jan
authors: Inam Ullah
authors: IU
authors: Khan
authors: Farhat
authors: F
authors: Ullah
authors: Salman A
authors: SA
authors: Malik
mesh_terms: D000293:Adolescent; D000328:Adult; D000953:Antigens, Protozoan / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D016054:DNA, Protozoan / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D016778:Malaria, Falciparum / Q000469:parasitology*; D016780:Malaria, Vivax / Q000469:parasitology*; D008297:Male; D020066:Merozoite Surface Protein 1 / Q000235:genetics; D008875:Middle Aged; D010154:Pakistan; D059208:Parasite Load; D018512:Parasitemia / Q000469:parasitology; D010963:Plasmodium falciparum / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010966:Plasmodium vivax / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015800:Protozoan Proteins / Q000235:genetics; D055815:Young Adult
chemical_list: D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D020066:Merozoite Surface Protein 1; D015800:Protozoan Proteins; C065974:merozoite surface protein 2, Plasmodium; C089133:merozoite surface protein 3, Plasmodium