title: Probing the site-selective binding of an antiretroviral drug, Stavudine to calf thymus DNA.
abstract: The interaction of an anti-HIV drug, stavudine (STV) with calf thymus deoxyribonucleic acid (DNA) was investigated employing acridine orange (AO) as a fluorescence probe. Spectroscopic investigations revealed the intercalative mode of binding of STV to DNA. The analysis of fluorescence data indicated the presence of static quenching mechanism between STV and DNA. Thermodynamic parameters indicated the presence of van der Waals forces in addition to intercalative mode of binding. CD data revealed the partial B → A conformational transition of DNA upon intercalative mode of binding with STV.
authors: a Department of Chemistry , Karnatak University , Dharwad , Karnataka , India.
authors: B
authors: B
authors: Sandhya
authors: J
authors: J
authors: Seetharamappa
mesh_terms: D000818:Animals; D019380:Anti-HIV Agents / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D001665:Binding Sites; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D055808:Drug Discovery; D007364:Intercalating Agents / Q000737:chemistry / Q000494:pharmacology*; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D018119:Stavudine / Q000737:chemistry / Q000494:pharmacology*; D013816:Thermodynamics
chemical_list: D019380:Anti-HIV Agents; D000970:Antineoplastic Agents; D007364:Intercalating Agents; D004247:DNA; C427203:calf thymus DNA; D018119:Stavudine