title: [Effect of high glucose peritoneal dialysis solution on PGC-1α expression and mitochondria related oxidative injury in human peritoneal mesothelial cells].
abstract: OBJECTIVE
  To investigate the mechanism of mitochondrial oxidative injury induced by high glucose peritoneal dialysis solution (PDS) and the protective effect of peroxisome proliferator activated receptor gamma coactivator 1-alpha (PGC-1α) in the mitochondria of human peritoneal mesothelial cells (HPMC) in the high glucose ambience.
  
  
  METHODS
  HPMC was cultured in a PDS containing 1.5%, 2.5% and 4.25% glucose for 24 hours. Western blot analysis was used to detect PGC-1α expression. MitoSOX? Red staining, respiratory chain complexes and antioxidant enzyme activities were determined.
  
  
  RESULTS
  The activities of respiratory chain complex III and antioxidant enzymes decreased significantly in a concentration- and time-dependent manner, along with the increased production of mitochondrial reactive oxygen species (ROS) and cellular apoptosis. In addition, protein expression of PGC-1α was also decreased in the high glucose PDS ambience.
  
  
  CONCLUSIONS
  High glucose PDS might inhibit PGC-1α expression, resulting in the inhibition of mitochondrial function and increase of mitochondrial ROS and cellular apoptosis.
authors: Department of Nephrology, Second Xiangya Hospital, Central South University; Kidney Institute, Central South University; Key Lab of Kidney Disease and Dialysis of Hunan Province, Changsha 410011, China.
authors: Xuejing
authors: X
authors: Zhu
authors: Feng
authors: F
authors: Wen
authors: Danyi
authors: D
authors: Yang
authors: Jing
authors: J
authors: Liu
authors: Shuguang
authors: S
authors: Yuan
authors: Jun
authors: J
authors: Li
authors: Hong
authors: H
authors: Liu
authors: Xiangqing
authors: X
authors: Xu
authors: Lin
authors: L
authors: Sun
authors: Fuyou
authors: F
authors: Liu
mesh_terms: D017209:Apoptosis; D015314:Dialysis Solutions / Q000009:adverse effects*; D004847:Epithelial Cells / Q000473:pathology*; D005947:Glucose / Q000009:adverse effects; D006801:Humans; D008928:Mitochondria / Q000378:metabolism / Q000473:pathology*; D018384:Oxidative Stress; D010530:Peritoneal Dialysis; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D017382:Reactive Oxygen Species; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D015314:Dialysis Solutions; C486497:PPARGC1A protein, human; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D017382:Reactive Oxygen Species; D014157:Transcription Factors; D005947:Glucose