title: Discovery and identification of serum potential biomarkers for pulmonary tuberculosis using iTRAQ-coupled two-dimensional LC-MS/MS.
abstract: Pulmonary tuberculosis (TB) caused by Mycobacterium tuberculosis is a chronic disease. Currently, there are no sufficiently validated biomarkers for early diagnosis of TB infection. In this study, a panel of potential serum biomarkers was identified between patients with pulmonary TB and healthy controls by using iTRAQ-coupled 2D LC-MS/MS technique. Among 100 differentially expressed proteins screened, 45 proteins were upregulated (>1.25-fold at p < 0.05) and 55 proteins were downregulated (<0.8-fold at p < 0.05) in the TB serum. Bioinformatics analysis revealed that the differentially expressed proteins were related to the response to stimulus, the metabolic and immune system processes. The significantly differential expression of apolipoprotein CII (APOCII), CD5 antigen-like (CD5L), hyaluronan-binding protein 2 (HABP2), and retinol-binding protein 4 (RBP4) was further confirmed using immunoblotting and ELISA analysis. By forward stepwise multivariate regression analysis, a panel of serum biomarkers including APOCII, CD5L, and RBP4 was obtained to form the disease diagnostic model. The receiver operation characteristic curve of the diagnostic model was 0.98 (sensitivity = 93.42%, specificity = 92.86%). In conclusion, APOCII, CD5L, HABP2, and RBP4 may be potential protein biomarkers of pulmonary TB. Our research provides useful data for early diagnosis of TB.
authors: Institute of Cell Biology, Zhejiang University, Hangzhou, P. R. China.
authors: Dan-Dan
authors: DD
authors: Xu
authors: Dan-Feng
authors: DF
authors: Deng
authors: Xiang
authors: X
authors: Li
authors: Li-Liang
authors: LL
authors: Wei
authors: Yan-Yuan
authors: YY
authors: Li
authors: Xiu-Yun
authors: XY
authors: Yang
authors: Wei
authors: W
authors: Yu
authors: Chong
authors: C
authors: Wang
authors: Ting-Ting
authors: TT
authors: Jiang
authors: Zhong-Jie
authors: ZJ
authors: Li
authors: Zhong-Liang
authors: ZL
authors: Chen
authors: Xing
authors: X
authors: Zhang
authors: Ji-Yan
authors: JY
authors: Liu
authors: Ze-Peng
authors: ZP
authors: Ping
authors: Yun-Qing
authors: YQ
authors: Qiu
authors: Ji-Cheng
authors: JC
authors: Li
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014397:Tuberculosis, Pulmonary / Q000097:blood* / Q000175:diagnosis; D055815:Young Adult
chemical_list: D015415:Biomarkers; D001798:Blood Proteins
keywords: Biomedicine; Potential biomarker; Pulmonary tuberculosis; Serum; iTRAQ-coupled 2D LC-MS/MS