title: Phytostabilization of moderate copper contaminated soils using co-inoculation of Vicia faba with plant growth promoting bacteria.
abstract: There is a need to conduct research on the selection of microbial isolates from rhizosphere of plants growing on heavy metal contaminated soils for specific restoration programs. This article suggest a consortium of bacteria combining Rhizobium sp. CCNWSX0481, Rhizobium leguminosarum bv. viciae, Enterobacter cloacae and Pseudomonas sp. 2(2010) that was examined for the ability to promote Vicia faba.L. growth when cultivated on the vineyard of soil moderately contaminated with copper. Data showed that inoculation was significant in nodulation; it increases the number and the weight of nodules of 50%. Co-inoculation was also found to positively influence growth and seed yield, through increasing fresh shoot and fresh root weights by 33 and 26%, respectively, and through rising numbers of seed per pod and pods per plant. In contrast, co-inoculation produced a significant reduction of accumulated copper in roots attending 35%, however, the treatment revealed no significant effects on the copper contents in pods and seeds. The tested inoculum could be an option to promote V. faba growth and to enhance soil fertilization in moderate copper contaminated soils. Further studies on the influence of co-inoculation practices on copper migration in soil-plant systems are recommended to acquire more information for evaluation of this legume safety.
authors: Laboratory of Legumes, University Tunis El Manar, Centre of Biotechnology of Borj Cedria, Hammam Lif, Tunisia.
authors: Imen Challougui
authors: IC
authors: Fatnassi
authors: Manel
authors: M
authors: Chiboub
authors: Omar
authors: O
authors: Saadani
authors: Moez
authors: M
authors: Jebara
authors: Salwa Harzalli
authors: SH
authors: Jebara
mesh_terms: D003300:Copper / Q000378:metabolism*; D016972:Enterobacter cloacae / Q000378:metabolism; D004787:Environmental Pollution; D019216:Metals, Heavy / Q000378:metabolism; D059013:Microbial Consortia; D008826:Microbial Sensitivity Tests; D063245:Plant Development; D055170:Plant Root Nodulation; D018517:Plant Roots / Q000254:growth & development; D011549:Pseudomonas / Q000378:metabolism; D012231:Rhizobium / Q000378:metabolism; D016961:Rhizobium leguminosarum / Q000378:metabolism; D012639:Seeds / Q000254:growth & development / Q000382:microbiology; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism; D013559:Symbiosis; D031307:Vicia faba / Q000254:growth & development* / Q000382:microbiology*
chemical_list: D019216:Metals, Heavy; D012989:Soil Pollutants; D003300:Copper
keywords: Bioremediation; Coinoculation; Copper stress; PGPB; Rhizobia; Vicia faba