title: Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe.
abstract: A label-free, oligonucleotide-based, switch-on luminescence detection method for T4 polynucleotide kinase activity has been developed using a novel G-quadruplex-selective luminescent Ir(iii) complex probe. The application of the assay for screening potential T4 PNK inhibitors is also demonstrated. To our knowledge, this is the first metal-based assay for PNK activity.
authors: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. edmondma@hkbu.edu.hk.
authors: Hong-Zhang
authors: HZ
authors: He
authors: Ka-Ho
authors: KH
authors: Leung
authors: Wei
authors: W
authors: Wang
authors: Daniel Shiu-Hin
authors: DS
authors: Chan
authors: Chung-Hang
authors: CH
authors: Leung
authors: Dik-Lung
authors: DL
authors: Ma
mesh_terms: D001681:Biological Assay; D015374:Biosensing Techniques; D056831:Coordination Complexes / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D054856:G-Quadruplexes; D006801:Humans; D007495:Iridium / Q000737:chemistry*; D008163:Luminescent Measurements; D010766:Phosphorylation; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000378:metabolism*
chemical_list: D056831:Coordination Complexes; D015342:DNA Probes; D004277:DNA, Single-Stranded; D007495:Iridium; D011116:Polynucleotide 5'-Hydroxyl-Kinase