title: Determination of cysteine and glutathione based on the inhibition of the dinuclear Cu(II)-catalyzed luminol-H2O2 chemiluminescence reaction.
abstract: The catalyzed luminol chemiluminescent reaction has received a great amount of attention because of its high sensitivity and low background signal which make the reaction an attractive analytical chemistry tool. The present study, introduces the beneficial catalytic effects of dinuclear Cu(II) complex [Cu2L2(TAE)]X2, where TAE=tetraacetylethane; L=N,N(')-dibenzylethylenediamine and X=ClO4 on the luminol chemiluminescent reaction as a novel probe for the determination of glutathione (GSH) and L-cysteine (CySH) in human serum and urine. The [Cu2L2(TAE)]X2 has exhibited highly efficient catalytic activity of luminol CL as an artificial peroxidase model at pH as low as 7.5 in water in the presence of H2O2⋅GSH and CySH can induce a sharp decrease in CL intensity from the [Cu2L2(TAE)]X2-catalyzed luminol system. Under the selected experimental conditions, a linear relationship was obtained between the CL intensity and the concentrations of GSH and CySH in the range of 1.0×10(-7)-1.0×10(-4) M, with detection limits (S/N=3) of 2.7×10(-8) and 6.8×10(-8) M and RSD<4.2% (n=7) for GSH and CySH, respectively.
authors: Analytical Division, Faculty of Chemistry, University of Mazandaran, Babolsar 4741695447, Iran. Electronic address: jchaichi@yahoo.com.
authors: Mohammad Javad
authors: MJ
authors: Chaichi
authors: Analytical Division, Faculty of Chemistry, University of Mazandaran, Babolsar 4741695447, Iran.
authors: Mahjoobeh
authors: M
authors: Ehsani
authors: Analytical Division, Faculty of Chemistry, University of Mazandaran, Babolsar 4741695447, Iran.
authors: Tahereh
authors: T
authors: Khajvand
authors: Analytical Division, Faculty of Chemistry, University of Mazandaran, Babolsar 4741695447, Iran.
authors: Hamid
authors: H
authors: Golchoubian
authors: Analytical Division, Faculty of Chemistry, University of Mazandaran, Babolsar 4741695447, Iran.
authors: Ehsan
authors: E
authors: Rezaee
mesh_terms: D002384:Catalysis; D056831:Coordination Complexes / Q000737:chemistry; D003300:Copper / Q000737:chemistry*; D003545:Cysteine / Q000097:blood* / Q000652:urine*; D005978:Glutathione / Q000097:blood* / Q000652:urine*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D049449:Luminescence; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods; D008165:Luminol / Q000737:chemistry*; D009195:Peroxidase / Q000737:chemistry
chemical_list: D056831:Coordination Complexes; D049408:Luminescent Agents; D008165:Luminol; D003300:Copper; D006861:Hydrogen Peroxide; D009195:Peroxidase; D005978:Glutathione; D003545:Cysteine
keywords: Dinuclear Cu(II) complex; Glutathione; Hydrogen peroxide; L-Cysteine; Luminol chemiluminescence