title: Protein L: a robust enzyme-conjugated molecule for detection of humanized single chain antibodies.
abstract: The current study was aimed at introducing an efficient enzyme-conjugated molecule for use in the detection of humanized single chain antibodies. Because they are composite in their variable domain sequences and also devoid of constant domains, humanized single chain antibodies require a suitable secondary enzyme-conjugated antibody (more precisely enzyme-conjugated protein molecule) to be efficiently recognized and detected in ELISA, dot blot, and other detection tests, guaranteeing a more precise evaluation of their quantity, affinity, and other features. In the current study we examined the ability of three HRP-conjugated protein molecules including protein L, anti-human IgG antibody, and anti-mouse IgG antibody in the detection of three humanized single chain antibodies (anti-CD20, anti-EGFRvIII, and anti-EGFR) in ELISA and dot blot tests. The results signify the HRP-conjugated protein L as the most efficient molecule for detecting humanized single chain antibody.
authors: 1 Biotechnology Research Center, Tabriz University of Medical Sciences , Tabriz, Iran .
authors: Yaghoub
authors: Y
authors: Safdari
authors: Safar
authors: S
authors: Farajnia
authors: Mohammad
authors: M
authors: Asgharzadeh
authors: Shiva Ahdi
authors: SA
authors: Khosroshahi
authors: Kamal
authors: K
authors: Veisi
authors: Vahideh
authors: V
authors: Ahmadzadeh
authors: Masoumeh
authors: M
authors: Khalili
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D057127:Single-Chain Antibodies / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; C055047:Ig L-binding protein, Peptostreptococcus; D057127:Single-Chain Antibodies; D006735:Horseradish Peroxidase