title: A new regulatory pathway of mRNA export by an F-box protein, Mdm30.
abstract: Mdm30, an F-box protein in yeast, has been recently shown to promote mRNA export. However, it remains unknown how Mdm30 facilitates mRNA export. Here, we show that Mdm30 targets the Sub2 component of the TREX (Transcription/Export) complex for ubiquitylation and subsequent proteasomal degradation. Such a targeted degradation of Sub2 enhances the recruitment of the mRNA export adaptor, Yra1, to the active genes to promote mRNA export. Together, these results elucidate that Mdm30 promotes mRNA export by lowering Sub2's stability and consequently enhancing Yra1 recruitment, thus illuminating new regulatory mechanisms of mRNA export by Mdm30.
authors: Geetha
authors: G
authors: Durairaj
authors: Shweta
authors: S
authors: Lahudkar
authors: Sukesh R
authors: SR
authors: Bhaumik
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000378:metabolism; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism*; D055786:Gene Knockout Techniques; D009687:Nuclear Proteins / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D055550:Protein Stability; D059748:Proteolysis; D012326:RNA Splicing; D034443:RNA Transport; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D054875:Ubiquitination
chemical_list: D044783:F-Box Proteins; C481653:Mdm30 protein, S cerevisiae; D009687:Nuclear Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; C105887:YRA1 protein, S cerevisiae; C516801:ADH1 protein, S cerevisiae; D000426:Alcohol Dehydrogenase; D046988:Proteasome Endopeptidase Complex; D000251:Adenosine Triphosphatases; C027900:RNA-dependent ATPase
keywords: Mdm30; Sub2; TREX; Yra1; mRNA export; transcription