title: Habituation to organic acid anions induces resistance to acid and bile in Listeria monocytogenes.
abstract: We evaluated the intrinsic and inducible resistance of four human pathogenic strains of Listeria monocytogenes to acid and bile, factors associated with virulence. Cells were grown in media at pH 7.4, or in media at pH 6.0 containing 0 (HCl control) or 4.75 mM of different organic acids, harvested at stationary or mid log phase, and challenged for 1h in acid or bile. Stationary phase cells were intrinsically more resistant to either challenge than log phase cells, and large differences between strains were evident among the latter. Compared to the HCl control, habituation to log phase with organic acids induced significant (p<0.05) and meaningful (≥1 log) increases in acid resistance of three of four strains tested, and in bile resistance of two strains suggesting that exposure to organic acid anions may enhance virulence in L. monocytogenes.
authors: Lab of Beef Processing and Quality Control, College of Food Science and Engineering, Shandong Agricultural University, Taian, Shandong 271018, PR China; Department of Nutrition, Dietetics and Food Sciences, Utah State University, Logan, UT 84322-8700, USA. Electronic address: ymzhangsdau@gmail.com.
authors: Yimin
authors: Y
authors: Zhang
authors: Department of Nutrition, Dietetics and Food Sciences, Utah State University, Logan, UT 84322-8700, USA.
authors: Charles E
authors: CE
authors: Carpenter
authors: Department of Nutrition, Dietetics and Food Sciences, Utah State University, Logan, UT 84322-8700, USA.
authors: Jeff R
authors: JR
authors: Broadbent
authors: Lab of Beef Processing and Quality Control, College of Food Science and Engineering, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors: Xin
authors: X
authors: Luo
mesh_terms: D000143:Acids / Q000737:chemistry*; D000838:Anions / Q000737:chemistry*; D001646:Bile / Q000737:chemistry*; D015169:Colony Count, Microbial; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D008089:Listeria monocytogenes / Q000254:growth & development / Q000502:physiology*; D037521:Virulence Factors
chemical_list: D000143:Acids; D000838:Anions; D037521:Virulence Factors
keywords: Acid anion; Acid resistance; Bile resistance; Listeria monocytogenes; Organic acid