title: A solid-phase fluorescent biosensor for the determination of phenolic compounds and peroxides in samples with complex matrices.
abstract: A solid-phase fluorescent biosensor for the determination of phenolic compounds (simple substituted phenols and catecholamines) and peroxides has been developed. The biosensor has a simple construction and the analytical signal is measured directly in a biosensitive layer {peroxidase-chitosan} on the sensor surface. This approach allowed analyzing samples with complex matrices (including water-insoluble samples and nontransparent solutions) without their preliminary pretreatment. Two novel fluorescent indicator reactions for the determination of the above-mentioned analytes in wide concentration ranges (from nmol l(-1) to mm l(-1)) which provided an analytical signal registration on a solid phase were proposed. The developed sensor was applied successfully for the analysis of urine, cosmetics, pharmaceuticals preparations, etc.
authors: Chemical Department, The Lomonosov Moscow State University, Moscow, 119991, Russia.
authors: P V
authors: PV
authors: Rodionov
authors: I A
authors: IA
authors: Veselova
authors: T N
authors: TN
authors: Shekhovtsova
mesh_terms: D015374:Biosensing Techniques; D002395:Catecholamines / Q000652:urine*; D048271:Chitosan / Q000737:chemistry; D003358:Cosmetics / Q000737:chemistry; D005456:Fluorescent Dyes; D006735:Horseradish Peroxidase / Q000737:chemistry; D042845:Laccase / Q000737:chemistry; D014442:Monophenol Monooxygenase / Q000737:chemistry; D010545:Peroxides / Q000032:analysis*; D010636:Phenols / Q000652:urine*; D012235:Rhodamines; D013050:Spectrometry, Fluorescence
chemical_list: D002395:Catecholamines; D003358:Cosmetics; D005456:Fluorescent Dyes; D010545:Peroxides; D010636:Phenols; D012235:Rhodamines; D048271:Chitosan; D042845:Laccase; D006735:Horseradish Peroxidase; D014442:Monophenol Monooxygenase; C029773:rhodamine B