title: Contribution of hot spring bacterial consortium in cadmium and lead bioremediation through quadratic programming model.
abstract: In the present investigation, a number of experiments have been conducted to isolate microbial strains from Taptapani Hot Spring Odisha, India for bioremediation of cadmium and lead. The strains Stenotrophomonas maltophilia (SS1), Aeromonas veronii (SS2) and Bacillus barbaricus (SS3) have shown better adaptation to metal tolerance test, with different concentrations of cadmium and lead and hence have been selected for further studies of metal microbial interaction and optimization. The results of bioremediation process indicate that consortium of thermophilic isolates adsorbed heavy metals more effectively than the individually treated isolates. Therefore, A 24 full factorial central composite design has been employed to analyze the effect of metal ion concentration, microbial concentration and time on removal of heavy metals with consortium. Analysis of variance (ANOVA) shows a high coefficient of determination value. The kinetic data have been fitted to pseudo-first order and second-order models. The isotherm equilibrium data have been well fitted by the Langmuir and Freundlich models. The optimum removal conditions determined for initial ion concentration was 0.3g/l; contact time 72h; microbial concentration, 3ml/l; and pH 7. At optimum adsorption conditions, the adsorption of cadmium and lead are found to be 92% and 93%, respectively, and presence of metals was confirmed through EDS analysis.
authors: Department of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur, Rayagada 765 022, Odisha, India.
authors: Sudip Kumar
authors: SK
authors: Sen
authors: Department of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur, Rayagada 765 022, Odisha, India.
authors: Sangeeta
authors: S
authors: Raut
authors: Department of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur, Rayagada 765 022, Odisha, India.
authors: Tapas Kumar
authors: TK
authors: Dora
authors: Department of Microbiology, Vidyasagar University, Midnapore 721 102, West Bengal, India. Electronic address: pkdmvu@gmail.com.
authors: Pradeep Kumar Das
authors: PK
authors: Mohapatra
mesh_terms: D000327:Adsorption; D001419:Bacteria / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000254:growth & development; D001673:Biodegradation, Environmental; D002104:Cadmium / Q000737:chemistry*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D045482:Hot Springs / Q000382:microbiology*; D007194:India; D007854:Lead / Q000737:chemistry*; D008962:Models, Theoretical; D062065:Wastewater; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000737:chemistry*
chemical_list: D002104:Cadmium; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007854:Lead; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Bioremediation; Cadmium; Hot spring; Isotherm; Lead; Optimization