title: BSA-boronic acid conjugate as lectin mimetics.
abstract: We report bovine serum albumin (BSA)-boronic acid (BA) conjugates as lectin mimetics and their glyco-capturing capacity. The BSA-BA conjugates were synthesized by amidation of carboxylic acid groups in BSA with aminophenyl boronic acid in the presence of EDC, and were characterized by Alizarin Red S (ARS) assay and SDS-PAGE gel. The BSA-BA conjugates were immobilized onto maleimide-functionalized silica beads and their sugar capturing capacity and specificity were confirmed by ARS displacement assay. Further, surface plasmon resonance (SPR) analysis of the glyco-capturing activity of the BSA-BA conjugates was conducted by immobilizing BSA-BA onto SPR gold chip. Overall, we demonstrated a BSA-BA-based lectin mimetics for glyco-capturing applications. These lectin mimetics are expected to provide an important tool for glycomics and biosensor research and applications.
authors: Department of Chemistry, Chemical and Biomedical Engineering, Center for Gene Regulation in Health and Disease (GRHD), Cleveland State University, 2121 Euclid Avenue, Cleveland, OH 44115, United States.
authors: Satya Nandana
authors: SN
authors: Narla
authors: Department of Chemistry, Chemical and Biomedical Engineering, Center for Gene Regulation in Health and Disease (GRHD), Cleveland State University, 2121 Euclid Avenue, Cleveland, OH 44115, United States.
authors: Poornima
authors: P
authors: Pinnamaneni
authors: School of Life Science and Technology, Harbin Institute of Technology, Harbin, Heilongjiang, China.
authors: Huan
authors: H
authors: Nie
authors: School of Life Science and Technology, Harbin Institute of Technology, Harbin, Heilongjiang, China.
authors: Yu
authors: Y
authors: Li
authors: Department of Chemistry, Chemical and Biomedical Engineering, Center for Gene Regulation in Health and Disease (GRHD), Cleveland State University, 2121 Euclid Avenue, Cleveland, OH 44115, United States. Electronic address: x.sun55@csuohio.edu.
authors: Xue-Long
authors: XL
authors: Sun
mesh_terms: D040761:Biomimetic Materials / Q000138:chemical synthesis*; D015374:Biosensing Techniques / Q000379:methods*; D001897:Boronic Acids / Q000737:chemistry*; D054794:Glycomics / Q000379:methods; D037102:Lectins / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D001897:Boronic Acids; D037102:Lectins; D011134:Polysaccharides; D012710:Serum Albumin, Bovine
keywords: 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide; ARS; Alizarin Red S; BA; BSA; Boronic acid; Bovine serum albumin; Carbohydrate; EDC; Lectin; SPR; Surface plasmon resonance; boronic acid; bovine serum albumin; surface plasmon resonance