title: Fasciola hepatica - the pilot study of in vitro assessing immune response against native and recombinant antigens of the fluke.
abstract: Fasciola hepatica is a liver fluke that infects 2.4 million of people and causes great economical loss in animal production. To date a 100% effective vaccine has not been developed and the disease is controlled by drug therapy. Great efforts are put into development of effective vaccine against parasite what is difficult since Fasciola spp. (like other helmints) during evolutionary process has developed sophisticated and efficient methods to evade immune response. During preliminary experiments it is convenient to use cell lines which are relatively cheap and allow for reproducible comparison of results between laboratories. We stimulated BOMA (bovine monocyte/macrophage cell line) and BOMAC (bovine macrophage cell line) with native or recombinant antigens of Fasciola hepatica and assessed IFN-γ, IL-4 and TNF-α level upon stimulation. We observed diminished secretion of proinflammatory TNF-α in LPS activated BOMA cells stimulated with Excretory/Secretory products of adult fluke (Fh-ES). We also observed greater changes in gene expression in LPS activated BOMA cells than in non activated BOMA cells upon stimulation using Fh-ES. The results show possibility of using cell lines for in vitro research of bovine immune response against liver fluke, although this model still requires validation and further characterization.
authors: Division of Pharmacology and Toxicology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, 8 Ciszewskiego Street, 02-786, Warsaw, Poland, piotr_baska@sggw.pl.
authors: Piotr
authors: P
authors: Bąska
authors: Anna
authors: A
authors: Zawistowska-Deniziak
authors: Anna M
authors: AM
authors: Zdziarska
authors: Katarzyna
authors: K
authors: Wasyl
authors: Marcin
authors: M
authors: Wiśniewski
authors: Anna
authors: A
authors: Cywińska
authors: Maciej
authors: M
authors: Klockiewicz
authors: Kamil
authors: K
authors: Januszkiewicz
authors: Halina
authors: H
authors: Wędrychowicz
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D002460:Cell Line; D005210:Fasciola hepatica / Q000235:genetics / Q000276:immunology*; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008264:Macrophages / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D010865:Pilot Projects; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D007371:Interferon-gamma