title: Comparison of HSCORE assessment of endometrial beta3 integrin subunit expression with digital HSCORE using computerized image analysis (ImageJ).
abstract: OBJECTIVE
  To compare intraobserver and interobserver variation between traditional histological score (HSCORE) and digital HSCORE (D-HSCORE) performed by expert and naive researchers.
  
  
  METHODS
  Immunohistochemical analysis of beta3 integrin subunit of 100 endometrial biopsies obtained from the midluteal phase of the menstrual cycle were reanalyzed using ImageJ software (D-HSCORE). Mean intensity of 3,3'-diaminobenzidine on endometrial glands was read by an expert (HSCORE) versus inexperienced observer using HSCORE and D-HSCORE.
  
  
  RESULTS
  The mean correlation [r(s)(95% CI)] between both methods was 0.86 (0.79-0.90) and highly significant (p < 0.0001) for the experienced individual. The naive researcher overestimated immunostaining, resulting (HSCORE) in negative samples. No discrepancies were seen with D-HSCORE. Interobserver variation for the inexperienced reader was 50% using HSCORE (cutoff 0.7) but 0% with D-HSCORE. Intraobserver variation using ImageJ was 0%.
  
  
  CONCLUSIONS
  The D-HSCORE performed by an inexperienced researcher has high correlation to traditional HSCORE performed by an expert.
authors: Department of Gynecology and Obstetrics, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors: Daniele G
authors: DG
authors: Fuhrich
authors: Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of South Carolina School of Medicine-Greenville, Greenville, South Carolina, USA.
authors: Bruce A
authors: BA
authors: Lessey
authors: Department of Gynecology and Obstetrics, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors: Ricardo F
authors: RF
authors: Savaris
mesh_terms: D000328:Adult; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods / Q000592:standards* / Q000706:statistics & numerical data; D007247:Infertility, Female / Q000378:metabolism / Q000473:pathology; D039661:Integrin beta3 / Q000378:metabolism*; D015588:Observer Variation; D010338:Pathology, Clinical / Q000379:methods / Q000592:standards* / Q000706:statistics & numerical data; D015203:Reproducibility of Results
chemical_list: D039661:Integrin beta3