title: Two-stage statistical medium optimization for augmented cellulase production via solid-state fermentation by newly isolated Aspergillus niger HN-1 and application of crude cellulase consortium in hydrolysis of rice straw.
abstract: Cellulolytic enzyme production by newly isolated Aspergillus niger HN-1 was statistically optimized using Plackett-Burman and central composite design (CCD). Optimum concentrations of 2, 0.40, 0.01, and 0.60 g L (-1) for KH2PO4, urea, trace elements solution, and CaCl2·2H2O, respectively, were suggested by Design-Expert software. The two-stage optimization process led to a 3- and 2-fold increases in the filter paper cellulase (FP) and β-glucosidase activities, respectively. FP, β-glucosidase, endoglucanase, exopolygalaturonase, cellobiohydrolase, xylanase, α-l-arabinofuranosidase, β-xylosidase, and xylan esterase activities of 36.7 ± 1.54 FPU gds(-1), 252.3 ± 7.4 IU gds(-1), 416.3 ± 22.8 IU gds(-1), 111.2 ± 5.4 IU gds(-1), 8.9 ± 0.50 IU gds(-1), 2593.5 ± 78.9 IU gds(-1), 79.4 ± 4.3 IU gds(-1), 180.8 ± 9.3 IU gds(-1), and 288.7 ± 11.8 IU gds(-1), respectively, were obtained through solid-state fermentation during the validation studies. Hydrolysis of alkali-treated rice straw with crude cellulases resulted in about 84% glucan to glucose, 89% xylan to xylose, and 91% arabinan to arabinose conversions, indicating potential for biomass hydrolysis by the crude cellulase consortium obtained in this study.
authors: Central Institute of Post-Harvest Engineering and Technology, P.O. PAU, Ludhiana 141 004, India.
authors: Simranjeet Kaur
authors: SK
authors: Sandhu
authors: Harinder Singh
authors: HS
authors: Oberoi
authors: Neha
authors: N
authors: Babbar
authors: Kanupriya
authors: K
authors: Miglani
authors: Bhupinder Singh
authors: BS
authors: Chadha
authors: Dhiraj Kumar
authors: DK
authors: Nanda
mesh_terms: D001234:Aspergillus niger / Q000145:classification / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D061249:Batch Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D055162:Biocatalysis; D044602:Cellulases / Q000096:biosynthesis* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D005285:Fermentation; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000378:metabolism; D006868:Hydrolysis; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry*; D010802:Phylogeny; D018547:Plant Stems / Q000378:metabolism / Q000382:microbiology; D012037:Refuse Disposal; D012988:Soil Microbiology
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D044602:Cellulases