title: Growth factor release from polyelectrolyte-coated titanium for implant applications.
abstract: Polyelectrolyte multilayer coatings based on poly(methacrylic acid) and poly-l-histidine were formed on anodized titanium surfaces with adsorbed bone morphogenetic protein 2 (BMP-2) or basic fibroblast growth factor (FGFb). These coatings are proposed for use on titanium implanted devices. Coatings were capable of sustained release of growth factor over 25 days, with BMP-2 and FGFb exhibiting approximately identical release profiles. Cell culture on growth factor-eluting surfaces was more effective for preosteoblasts on BMP-2-eluting surfaces than for fibroblasts on FGFb-eluting surfaces. Cell counts at all time points on BMP-2-eluting surfaces were significantly higher than for those on anodized titanium or polyelectrolyte surfaces that did not contain BMP-2. Alkaline phosphatase levels were significantly higher after 21 days on BMP-2-eluting surfaces, indicating increased bone growth.
authors: Interfaces Department, Max Planck Institute of Colloids and Interfaces , Am Mühlenberg 1, 14476 Potsdam, Germany.
authors: Amy M
authors: AM
authors: Peterson
authors: Christine
authors: C
authors: Pilz-Allen
authors: Tatiana
authors: T
authors: Kolesnikova
authors: Helmuth
authors: H
authors: Möhwald
authors: Dmitry
authors: D
authors: Shchukin
mesh_terms: D000327:Adsorption; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006639:Histidine / Q000737:chemistry*; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D011109:Polymethacrylic Acids / Q000737:chemistry*; D019736:Prostheses and Implants; D058525:Quartz Crystal Microbalance Techniques; D014025:Titanium
chemical_list: D055396:Bone Morphogenetic Protein 2; D020099:Coated Materials, Biocompatible; D004573:Electrolytes; D011109:Polymethacrylic Acids; D016222:Fibroblast Growth Factor 2; C033223:polyhistidine; D006639:Histidine; D014025:Titanium; D000469:Alkaline Phosphatase; D016650:Fluorescein-5-isothiocyanate