title: Reorientation of the helix of the tryptophan-rich gp41W peptide from HIV-1 at interfaces.
abstract: The glycoprotein gp41 from the Human Immunodeficiency Virus type 1 (HIV-1) has an amino acid sequence enriched in tryptophan residues, the so-called gp41W peptide (i.e., KWASLWNWFNITNWLWYIK) and plays a crucial role in HIV-1 host cell infection. Using the coupling of Second Harmonic Generation targeting the tryptophan residues with lateral surface tension measurements, we investigate the interaction of gp41W with a neat air∕water and a lipid∕water interfaces. At the air∕water interface, gp41W presents a well-defined orientation and this orientation is strongly modified at the lipid∕water interface, depending on the surface pressure. These results show that this strategy is well suited to monitor tryptophan containing α-helices orientation at lipid∕water interfaces.
authors: Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, UMR CNRS 5246, CPE Lyon, INSA Lyon, Université Claude Bernard Lyon1, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne cedex, France.
authors: Gladys
authors: G
authors: Matar
authors: Institut Lumière Matière, UMR5306 Université Lyon 1-CNRS, Université de Lyon 69622 Villeurbanne cedex, France.
authors: Emmanuel
authors: E
authors: Benichou
authors: Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, UMR CNRS 5246, CPE Lyon, INSA Lyon, Université Claude Bernard Lyon1, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne cedex, France.
authors: Mehmet Nail
authors: MN
authors: Nasir
authors: Institut Lumière Matière, UMR5306 Université Lyon 1-CNRS, Université de Lyon 69622 Villeurbanne cedex, France.
authors: Yara
authors: Y
authors: El Harfouch
authors: Institut Lumière Matière, UMR5306 Université Lyon 1-CNRS, Université de Lyon 69622 Villeurbanne cedex, France.
authors: Pierre-François
authors: PF
authors: Brevet
authors: Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, UMR CNRS 5246, CPE Lyon, INSA Lyon, Université Claude Bernard Lyon1, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne cedex, France.
authors: Françoise
authors: F
authors: Besson
mesh_terms: D000327:Adsorption; D000388:Air; D000595:Amino Acid Sequence; D015700:HIV Envelope Protein gp41 / Q000737:chemistry*; D015497:HIV-1; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry*; D010715:Phosphatidylglycerols / Q000737:chemistry; D017433:Protein Structure, Secondary; D014364:Tryptophan; D014867:Water / Q000737:chemistry
chemical_list: D015700:HIV Envelope Protein gp41; D010446:Peptide Fragments; D010715:Phosphatidylglycerols; C514787:gp41 protein, Human immunodeficiency virus 1; D014867:Water; D014364:Tryptophan; C030345:1,2-dipalmitoylphosphatidylglycerol