title: microRNA expression in hepatitis B virus infected primary treeshrew hepatocytes and the independence of intracellular miR-122 level for de novo HBV infection in culture.
abstract: Infection of Hepatitis B virus (HBV) in hepatocytes has been known to be controlled by multiple cellular factors, while the relationship of the infection and liver microRNAs remains obscure. In this study, a miRNA database, containing 168 unique mature miRNA members from primary hepatocytes of a primate-like animal, northern treeshrew (Tupaia belangeri) that is the only species susceptible for HBV infection other than human and chimpanzee, was established. The relative level of a liver predominant microRNA, miR-122, was markedly increased upon HBV infection of the primary tupaia hepatocyte (PTH). However, introducing neither miR-122 nor its antagonist anti-miR-122 into PTHs, or, HepG2-NTCP that is HepG2 cells with the newly identified receptor sodium taurocholate cotransporting polypeptide (NTCP) did not alter the viral infection on these cells. These data suggest that de novo HBV infection of cultured hepatocytes does not depend on the expression level of intracellular miR-122 of the target cells.
authors: School of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences, 5 Dongdan Santiao, Beijing 100005, China; National Institute of Biological Sciences, Beijing, 7 Science Park Road, Zhongguancun Life Science Park, Changping, Beijing 102206, China.
authors: Guangwei
authors: G
authors: Xu
authors: Zhenchao
authors: Z
authors: Gao
authors: Wenhui
authors: W
authors: He
authors: Yanting
authors: Y
authors: Ma
authors: Xiaofeng
authors: X
authors: Feng
authors: Tao
authors: T
authors: Cai
authors: Fengmin
authors: F
authors: Lu
authors: Li
authors: L
authors: Liu
authors: Wenhui
authors: W
authors: Li
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D006509:Hepatitis B / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D006515:Hepatitis B virus / Q000235:genetics / Q000502:physiology*; D022781:Hepatocytes / Q000378:metabolism* / Q000821:virology; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000821:virology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D014417:Tupaia / Q000235:genetics / Q000378:metabolism / Q000821:virology
chemical_list: D035683:MicroRNAs
keywords: De novo infection; Hepatitis B virus; Hepatocyte; MicroRNA database; MicroRNA-122; Pathogenesis; RNA deep sequencing; Sodium taurocholate cotransporting polypeptide (NTCP); Tupaia