title: Cell cycle analysis and interspecies nuclear transfer of cat cells treated with chemical inhibitors.
abstract: This study investigated the effect of chemical inhibitors on the cell-cycle synchronisation in cat fibroblast cells and evaluated the development of interspecies embryos reconstructed from cat donor cells and enucleated bovine oocytes. Cat fibroblast cells were treated with 15 μg/mL roscovitine or 0.05 μg/mL deme-colcine prior to cell cycle analysis and nuclear transfer. The percentage of cat fibroblast cells arrested at the G0/G1 phase in the roscovitine group was similar to that in the control group without any treatment. The percentage of cells arrested at the G2/M phase was significantly higher in the demecolcine group than in the control group. The fusion rate of interspecies couplets was significantly greater in the roscovitine group than in the control group. Most embryos stopped the development at the 2- or 4-cell stage, and none developed into blastocysts. Chemical inhibitor-induced donor cell cycle synchronisation did not overcome developmental arrest in interspecies cloned embryos.
authors: Yamaguchi University The United Graduate School of Veterinary Science Yamaguchi 753-8515 Japan Chulalongkorn University Faculty of Veterinary Sciences Bangkok 10330 Thailand.
authors: Manita
authors: M
authors: Wittayarat
authors: Yamaguchi University The United Graduate School of Veterinary Science Yamaguchi 753-8515 Japan.
authors: Akira
authors: A
authors: Fujiwara
authors: Chulalongkorn University Faculty of Veterinary Sciences Bangkok 10330 Thailand.
authors: Kaywalee
authors: K
authors: Chatdarong
authors: Chulalongkorn University Faculty of Veterinary Sciences Bangkok 10330 Thailand.
authors: Mongkol
authors: M
authors: Techakumphu
authors: Yamaguchi University The United Graduate School of Veterinary Science Yamaguchi 753-8515 Japan.
authors: Yoko
authors: Y
authors: Sato
authors: Yamaguchi University The United Graduate School of Veterinary Science Yamaguchi 753-8515 Japan.
authors: Fuminori
authors: F
authors: Tanihara
authors: Yamaguchi University The United Graduate School of Veterinary Science Yamaguchi 753-8515 Japan.
authors: Yasuhiro
authors: Y
authors: Morita
authors: Yamaguchi University The United Graduate School of Veterinary Science Yamaguchi 753-8515 Japan.
authors: Masayasu
authors: M
authors: Taniguchi
authors: Yamaguchi University The United Graduate School of Veterinary Science Yamaguchi 753-8515 Japan.
authors: Takeshige
authors: T
authors: Otoi
keywords: Bovine oocyte; cell cycle synchronisation; demecolcine; feline; roscovitine