title: Production of tropane alkaloids in Hyoscyamus niger (black henbane) hairy roots grown in bubble-column and spray bioreactors.
abstract: Hairy root cultures of Hyoscyamus niger were cultivated in shake-flasks, a bubble-column bioreactor and a hybrid bubble-column/spray bioreactor and evaluated for alkaloid production. The latter gave the highest anisodamine content (0.67 mg/g dry wt) whereas scopolamine, hyoscyamine and cuscohygrine concentrations were highest in the bubble-column reactor (5.3, 1.6 and 26.5 mg/g dry wt, respectively). Both bioreactors gave similar productivities of scopolamine (1 and 0.98 mg/l day) and cuscohygrine (5 and 5.4 mg/l day), but anisodamine productivity was 3.5-fold higher in the hybrid bioreactor (HB) (0.02 and 0.07 mg/l day, respectively). Elicitation with methyl jasmonate increased scopolamine productivity by 146 % in roots grown in the HB whereas their permeabilization with DMSO caused 4-, 5-, 25- and 28-fold increase in scopolamine, hyoscyamine, anisodamine and cuscohygrine concentrations in the growth medium. In situ extraction with Amberlite XAD-2 doubled scopolamine productivity in the hybrid reactor after 50 days.
authors: Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdansk, Al. Gen. J. Hallera 107, 80-416, Gdańsk, Poland, zbigniew.jaremicz@gumed.edu.pl.
authors: Zbigniew
authors: Z
authors: Jaremicz
authors: Maria
authors: M
authors: Luczkiewicz
authors: Adam
authors: A
authors: Kokotkiewicz
authors: Aleksandra
authors: A
authors: Krolicka
authors: Pawel
authors: P
authors: Sowinski
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification / Q000378:metabolism*; D019149:Bioreactors; D003517:Cyclopentanes / Q000378:metabolism; D032304:Hyoscyamus / Q000378:metabolism*; D054883:Oxylipins / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism*; D014326:Tropanes / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000470:Alkaloids; D003517:Cyclopentanes; D054883:Oxylipins; D014326:Tropanes; C011006:jasmonic acid