title: The essential role of clathrin-mediated endocytosis in yellow head virus propagation in the black tiger shrimp Penaeus monodon.
abstract: Yellow head virus (YHV) is one of the most widespread viruses seriously affecting black tiger shrimp (Penaeus monodon) cultivation. A previous microarray study demonstrated that clathrin coat assembly protein 17 (AP17) was significantly up-regulated after YHV infection (Pongsomboon et al., 2011). Clathrin coat AP17 is a part of the assembly protein σ2 (AP-2) complex which is involved in clathrin-mediated endocytosis. Quantitative RT-PCR (qRT-PCR) revealed that the clathrin coat AP17 gene was up-regulated 3-fold at 12 h post YHV infection. In addition, immunofluorescence microscopy showed that clathrin coat AP17 was highly expressed in the cytoplasm of the YHV-infected hemocytes. Knockdown of the clathrin coat AP17 gene dramatically reduced YHV replicativity by 32-fold. Interestingly, shrimp pre-treated with chlorpromazine, a commercial drug that inhibits clathrin-dependent endocytosis, exhibited significantly low levels of YHV infection. Taken together, these results suggest that clathrin-mediated endocytosis is involved in YHV propagation in P. monodon.
authors: Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors: Thapanan
authors: T
authors: Jatuyosporn
authors: Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, Thailand.
authors: Premruethai
authors: P
authors: Supungul
authors: Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors: Anchalee
authors: A
authors: Tassanakajon
authors: Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: Kuakarun.K@chula.ac.th.
authors: Kuakarun
authors: K
authors: Krusong
mesh_terms: D033962:Adaptor Protein Complex 2 / Q000235:genetics / Q000378:metabolism*; D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D002746:Chlorpromazine / Q000008:administration & dosage / Q000494:pharmacology; D002966:Clathrin / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D004705:Endocytosis / Q000187:drug effects; D006434:Hemocytes / Q000276:immunology* / Q000821:virology; D030341:Nidovirales Infections / Q000276:immunology*; D033561:Penaeidae / Q000276:immunology*; D021381:Protein Transport; D034741:RNA, Small Interfering / Q000235:genetics; D046828:Roniviridae / Q000502:physiology*; D015854:Up-Regulation; D014779:Virus Replication / Q000187:drug effects / Q000235:genetics
chemical_list: D033962:Adaptor Protein Complex 2; D033942:Adaptor Proteins, Vesicular Transport; D002966:Clathrin; D034741:RNA, Small Interfering; D002746:Chlorpromazine
keywords: Clathrin coat assembly protein 17; Clathrin-mediated endocytosis; Penaeus monodon; YHV; Yellow head virus