title: Induction of micronuclei and nuclear lesions in Channa punctatus following exposure to carbosulfan, glyphosate and atrazine.
abstract: The genotoxic effects of commonly used agricultural pesticides viz., carbosulfan, glyphosate, and atrazine, were evaluated in Channa punctatus (Pisces, Perciformes) using micronucleus (MN) test and induction of nuclear lesions (NL). The 96 h LC50 value were estimated by probit analysis as 0.27, 32.0 and 42.0 mg L(-1), respectively, for carbosulfan, glyphosate, and atrazine using semi-static bioassays. Based on these values, three sublethal test concentrations of carbosulfan (0.07, 0.13, 0.20 mg L(-1)), glyphosate (8.1, 16.3, 24.4 mg L(-1)) and atrazine (10.6, 21.2, 31.8 mg L(-1)) corresponding to ¼, ½ and ¾ of the LC50 of the pesticides respectively, were selected for exposure for 96 h. Peripheral blood samplings were taken at intervals of 24 h for assessment of MN and NL frequencies. Considerably higher genotoxic damage was induced by carbosulfan as compared to glyphosate and atrazine. There were significant effects (p < 0.01) of concentrations in all the treated groups. The induction of MN and NL was highest at 96 h pesticide exposure at all test concentrations. The nuclear abnormalities recorded in this study, such as blebbed-, lobed-, notched- and bi-nuclei, other than micronuclei, are indicators of genotoxic damage.
authors: Molecular Biology and Biotechnology Division, Department of Zoology and Environmental Biology, University of Nigeria Nsukka , Enugu State , Nigeria .
authors: Christopher Didigwu
authors: CD
authors: Nwani
authors: Naresh Sahebrao
authors: NS
authors: Nagpure
authors: Ravindra
authors: R
authors: Kumar
authors: Basdeo
authors: B
authors: Kushwaha
authors: Pavan
authors: P
authors: Kumar
authors: Wazir Singh
authors: WS
authors: Lakra
mesh_terms: D000818:Animals; D001280:Atrazine / Q000008:administration & dosage / Q000633:toxicity*; D002219:Carbamates / Q000008:administration & dosage / Q000633:toxicity*; D005998:Glycine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000633:toxicity; D006540:Herbicides / Q000633:toxicity; D007928:Lethal Dose 50; D015162:Micronucleus Tests; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity*; D010473:Perciformes; D010575:Pesticides / Q000633:toxicity; D000097797:Glyphosate
chemical_list: D001280:Atrazine; D002219:Carbamates; D005998:Glycine; D006540:Herbicides; D009153:Mutagens; D010575:Pesticides; D000097797:Glyphosate; C035038:carbosulfan
keywords: Atrazine; Channa punctatus; carbosulfan; genotoxicity; glyphosate; micronucleus test; nuclear lesions