title: Genetically-engineered human neural stem cells with rabbit carboxyl esterase can target CNS lymphoma.
abstract: BACKGROUND
  Despite advances in its treatment, CNS lymphoma remains a devastating disease. Taking advantage of the tumour-tropic properties of neural stem cells (NSCs) is a novel therapeutic strategy. To apply this strategy to the treatment of CNS lymphoma, we investigated the role of NSCs expressing carboxyl esterase (HB1.F3.CE), which activates irinotecan.
  
  
  METHODS
  In order to find in vitro bystander effects of engineered NSCs, we performed cell viability assays. In vivo, the HB1.F3.CE cells were injected into the brain of mice with orthotopic CNS lymphoma. Mice were then treated with irinotecan by systemic administration.
  
  
  RESULTS
  The HB1.F3.CE cells significantly inhibited the growth of Raji cells with irinotecan treatment. In vivo, the HB1.F3.CE cells migrated into the tumour and significantly reduced tumour volume. In addition, survival of mice was prolonged by treatment with HB1.F3.CE and irinotecan.
  
  
  CONCLUSIONS
  Transplantation of human NSCs encoding CE into brain, combined with irinotecan therapy, may be an effective treatment regimen for CNS lymphoma.
authors: Department of Neurosurgery, Samsung Medical Centre and Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Seoul 135-710, South Korea. Tel: +82 234103497, nsnam@skku.edu.
authors: Wonyoung
authors: W
authors: Kang
authors: Juyoun
authors: J
authors: Jin
authors: Heekyoung
authors: H
authors: Yang
authors: Ho Jun
authors: HJ
authors: Seol
authors: Kang Ho
authors: KH
authors: Kim
authors: Jung-Ii
authors: JI
authors: Lee
authors: Seung U
authors: SU
authors: Kim
authors: Kyeung Min
authors: KM
authors: Joo
authors: Do-Hyun
authors: DH
authors: Nam
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic; D001483:Base Sequence; D024201:Bystander Effect; D002166:Camptothecin / Q000031:analogs & derivatives / Q000494:pharmacology; D043182:Carboxylesterase / Q000235:genetics*; D016543:Central Nervous System Neoplasms / Q000188:drug therapy / Q000473:pathology / Q000628:therapy*; D017931:DNA Primers; D005818:Genetic Engineering; D006801:Humans; D000077146:Irinotecan; D008223:Lymphoma / Q000188:drug therapy / Q000473:pathology / Q000628:therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D058953:Neural Stem Cells / Q000166:cytology* / Q000201:enzymology; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D017931:DNA Primers; D000077146:Irinotecan; D043182:Carboxylesterase; D002166:Camptothecin
keywords: CNS lymphoma; Irinotecan; carboxyl esterase (CE); neural stem cells; rabbit