title: Effect of gold nanoparticles on production of reactive oxygen species by human peripheral blood leukocytes stimulated with opsonized zymosan.
abstract: We studied the effect of gold nanoparticles on ROS production by leukocytes. ROS production was detected by luminol-dependent chemiluminescence (LDCL) of human peripheral blood leukocytes stimulated with opsonized zymosan. Nanoparticle size was 5, 10 and 30 nm. Simultaneous addition of nanoparticles and opsonized zymosan showed that 5-nm nanoparticles inhibited LDCL intensity in comparison with the control, when LDCL recording was conducted in the presence of opsonized zymosan. Increasing nanoparticle size from 5 up to 30 nm enhanced LDCL intensity. Preincubation of gold nanoparticles with autologous blood plasma increased LDCL intensity. In the control (without gold nanoparticles), blood plasma produced no activating effect on LDCL. We found that the effect of gold nanoparticles on leukocyte LDCL depended on nanoparticle size: 10- and 30-nm nanoparticles inhibited LDCL intensity in comparison with the control (incubation in the absence of nanoparticles) irrespective of the duration of incubation, while 5-nm gold nanoparticles had no effect on LDCL intensity. Incubation of gold nanoparticles with autologous plasma increased LDCL intensity if nanoparticle size was 30 and 10 nm.
authors: Laboratory of Biophysical Bases of Pathology, Research Institute of Physicochemical Medicine, Ministry of Health Care and Social Development, Moscow, Russia. aseychev@mail.ru.
authors: A P
authors: AP
authors: Piryazev
authors: O A
authors: OA
authors: Azizova
authors: A V
authors: AV
authors: Aseichev
authors: L B
authors: LB
authors: Dudnik
authors: V I
authors: VI
authors: Sergienko
mesh_terms: D002478:Cells, Cultured; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D008163:Luminescent Measurements; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D009895:Opsonin Proteins / Q000737:chemistry; D010316:Particle Size; D010587:Phagocytosis; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015054:Zymosan / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D009895:Opsonin Proteins; D017382:Reactive Oxygen Species; D006046:Gold; D015054:Zymosan