title: Characterization of an immunologically active pectin from the fruits of Lycium ruthenicum.
abstract: An immunologically active pectin, named LRGP5, was firstly isolated from the fruits of Lycium ruthenicum Murr. It contained rhamnose, arabinose, xylose, galactose and galacturonic acid in the molar ratio of 1.0:2.2:0.5:1.2:4.7. Its molecular weight was estimated to be approximately 1.37 × 10(5)Da by high performance gel permeation chromatography. The structure was elucidated using methylation analysis, partial acid hydrolysis, NMR and ESI-MS analysis. Results showed that LRGP5 consisted of a (1 → 4)-linked galacturonic acid backbone occasionally interrupted by (1 → 2)-linked rhamnose. The side chains were attached to position 4 of the rhamnosyl units, including (1 → 3)-linked arabinose, (1 → 3)-linked galactose, (1 → 3,6)-linked galactose, (1 → 4)-linked galacturonic acid, (1 → 2)-linked rhamnose and (1 → 2,4)-linked rhamnose, and the termini were arabinose and rhamnose. Immunological assay results demonstrated that LRGP5 could significantly promote macrophage proliferation and enhance the secretion of nitrogen monoxide in vitro.
authors: Natural Products and Glycoconjugate Research Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China; College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China.
authors: Qiang
authors: Q
authors: Peng
authors: Natural Products and Glycoconjugate Research Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
authors: Qingsong
authors: Q
authors: Xu
authors: Natural Products and Glycoconjugate Research Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
authors: Heng
authors: H
authors: Yin
authors: Educational Ministry Key Laboratory of Resource Biology and Biotechnology in Western China, Life Science College, Northwest University, Xi'an 710069, China. Electronic address: huanglj@nwu.edu.cn.
authors: Linjuan
authors: L
authors: Huang
authors: Natural Products and Glycoconjugate Research Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China. Electronic address: articles1805@gmail.com.
authors: Yuguang
authors: Y
authors: Du
mesh_terms: D000818:Animals; D002460:Cell Line; D005638:Fruit / Q000737:chemistry*; D006868:Hydrolysis; D056747:Immunomodulation / Q000187:drug effects*; D032305:Lycium / Q000737:chemistry*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008970:Molecular Weight; D010368:Pectins / Q000737:chemistry* / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology
chemical_list: D010936:Plant Extracts; D010368:Pectins
keywords: Immunomodulating activity; Lycium ruthenicum; Pectin; Polysaccharide; Structure