title: Anti-inflammatory and antifibrotic effects of a combination of spiperone and immobilized hyaluronidase on partially reversible and irreversible toxic pneumofibrosis.
abstract: The possibility of boosting antifibrotic activity of testicular hyaluronidase immobilized on polyethylene oxide with spiperone was studied on the bleomycin models of a single (partially reversible pneumofibrosis) and repeated (irreversible pneumofibrosis) injuries to the alveolar epithelium in C57Bl/6 mice. The antifibrotic effect was more pronounced after successive treatment with immobilized hyaluronidase and spiperone than after individual treatment with each of the compounds: no collagen deposition in the parenchyma of bleomycin-damaged lungs was found. The decrease in inflammatory cell (lymphocytes, macrophages, neutrophils, plasma cells) infiltration of the alveoli and alveolar tracts interstitium in mice treated by immobilized hyaluronidase and spiperone did not differ from the anti-inflammatory effect of spiperone monotherapy.
authors: Research Institute of Pharmacology, Siberian Division of the Russian Academy of Medical Sciences, Tomsk; Scientific Future Management, Novosibirsk, Russia. ovpershina@gmail.com.
authors: E G
authors: EG
authors: Skurikhin
authors: N N
authors: NN
authors: Ermakova
authors: A M
authors: AM
authors: Reztsova
authors: O V
authors: OV
authors: Pershina
authors: E S
authors: ES
authors: Khmelevskaya
authors: V A
authors: VA
authors: Krupin
authors: I E
authors: IE
authors: Stepanova
authors: V M
authors: VM
authors: Reztsova
authors: D V
authors: DV
authors: Reihard
authors: A V
authors: AV
authors: Artamonov
authors: A A
authors: AA
authors: Bekarev
authors: P G
authors: PG
authors: Madonov
authors: D N
authors: DN
authors: Kinsht
authors: V E
authors: VE
authors: Goldberg
authors: A M
authors: AM
authors: Dygai
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001761:Bleomycin; D003094:Collagen / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D004359:Drug Therapy, Combination; D004800:Enzymes, Immobilized / Q000494:pharmacology / Q000627:therapeutic use; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology* / Q000627:therapeutic use; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000188:drug therapy* / Q000517:prevention & control; D013134:Spiperone / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000893:Anti-Inflammatory Agents; D004800:Enzymes, Immobilized; D001761:Bleomycin; D013134:Spiperone; D003094:Collagen; D006821:Hyaluronoglucosaminidase