title: Production and degradation of N-acylhomoserine lactone quorum sensing signal molecules in bacteria isolated from activated sludge.
abstract: N-Acylhomoserine lactones (AHLs) function as quorum-sensing signaling molecules in many Gram-negative bacteria. We isolated a total of 672 bacterial strains from activated sludge obtained from seven sewage treatment plants in Tochigi Prefecture, Japan, and screened for AHL-producing and degrading strains. Isolates (n = 107) stimulated AHL-mediated purple pigment production in AHL reporter strains Chromobacterium violaceum CV026 and VIR07. Based on their 16S rRNA gene sequences, most of these AHL-producing isolates were assigned to the genus Aeromonas, and they were divided into six groups. Isolates (n = 46) degraded N-decanoyl-L-homoserine lactone (C10-HSL) within 24 h. Based on their 16S rRNA gene sequences, the most dominant AHL-degrading isolates were assigned to the genus Acinetobacter and divided into six groups. Strains Ooi24, Omo91, and Uzu81, which showed higher C10-HSL-degrading activity, showed putative AHL-acylase activity.
authors: Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University.
authors: Seiji
authors: S
authors: Ochiai
authors: Tomohiro
authors: T
authors: Morohoshi
authors: Ayane
authors: A
authors: Kurabeishi
authors: Masahiro
authors: M
authors: Shinozaki
authors: Haruka
authors: H
authors: Fujita
authors: Isao
authors: I
authors: Sawada
authors: Tsukasa
authors: T
authors: Ikeda
mesh_terms: D000150:Acinetobacter / Q000166:cytology / Q000302:isolation & purification / Q000378:metabolism; D054742:Acyl-Butyrolactones / Q000378:metabolism*; D001419:Bacteria / Q000166:cytology / Q000302:isolation & purification* / Q000378:metabolism*; D053038:Quorum Sensing; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000382:microbiology
chemical_list: D054742:Acyl-Butyrolactones; D012722:Sewage; D062065:Wastewater