title: [Overexpression of Wnt3a inhibited the apoptosis of mouse embryonic liver stem cells].
abstract: OBJECTIVE
  To study the effect of Wnt3a on the apoptosis and the expressions of apoptosis-related proteins in mouse embryonic liver stem cells (ELSCs).
  
  
  METHODS
  The mouse ELSC14.5 cells were randomly divided into two groups: control group and experimental group, which were infected with two adenovirus vectors expressing green fluorescent protein, Ad-GFP and Ad-GFP-wnt3a, respectively. The mRNA and protein expressions of Wnt3a and apoptosis-related factors Bcl-2, Mcl-1 and Bax were examined by real-time quantitative PCR (qRT-PCR) and Western blotting respectively. Cell apoptosis was tested by annexin-PE/7-ADD and Hoechst 33258 staining method.
  
  
  RESULTS
  qRT-PCR and Western blotting showed Wnt3a was effectively expressed in ELSC14.5 cells, indicating the adenovirus vector Ad-Wnt3a had been successfully transferred into the targeted cells. Compared with the control group, the experimental group presented the increased mRNA and protein expressions of the anti-apoptotic factor Bcl-2 and Mcl-1, and the decreased expression of pro-apoptotic factor Bax (P < 0.05). Hoechst 33258 staining method combined with annexin-PE/7-ADD method showed that the ELSC14.5 cells highly expressing Ad-Wnt3a, whose nuclei were dense, hyperchromatic, or fragmented, were very rare, and that the rate of cell apoptosis was reduced.
  
  
  CONCLUSIONS
  Wnt3a inhibites the mouse ELSC apoptosis by up-regulating the Bcl-2 family's anti-apoptotic and down-regulating pro-apoptotic factors to prolong its survival.
authors: Research Center of Molecular Medicine and Cancer, College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China.
authors: Xifeng
authors: X
authors: Zhang
authors: Daixi
authors: D
authors: Hu
authors: Yongliang
authors: Y
authors: Lu
authors: Tao
authors: T
authors: Feng
authors: Jiayi
authors: J
authors: Huang
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D017209:Apoptosis; D002467:Cell Nucleus / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology*; D015870:Gene Expression; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006801:Humans; D008099:Liver / Q000166:cytology*; D051379:Mice; D064549:Myeloid Cell Leukemia Sequence 1 Protein / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D014161:Transduction, Genetic; D060509:Wnt3A Protein / Q000235:genetics*; D051028:bcl-2-Associated X Protein / Q000235:genetics
chemical_list: C579095:MCL1 protein, human; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D019253:Proto-Oncogene Proteins c-bcl-2; D060509:Wnt3A Protein; D051028:bcl-2-Associated X Protein