title: Distribution of glucagon-like peptide (GLP)-2-immunoreactive cells in the chicken small intestine: antigen retrieval immunohistochemistry.
abstract: An antigen retrieval method for immunohistochemical staining of glucagon-like peptide (GLP)-2-immunoreactive cells was investigated in the chicken small intestine. GLP-2-immunoreactive cells were observed as open-typed endocrine cells in the villous epithelium and crypts on both antigen retrieval agent-treated and untreated preparations. No obvious differences were detected in morphological features of GLP-2-immunoreactive cells between treated and untreated preparations. The frequencies of occurrence of GLP-2-immunoreactive cells, however, were significantly different in treated and untreated preparations: in the proximal and distal regions of jejunum and ileum obtained from untreated preparations, the frequencies of occurrence were 0.5 ± 0.2, 0.7 ± 0.1, 0.9 ± 0.2 and 1.5 ± 0.3, respectively (cell numbers per mucosal area: cells/mm(2), mean ± SD), whereas those from treated sections were 14.7 ± 2.3, 19.8 ± 2.3, 23.5 ± 4.7 and 34.6 ± 4.9 cells/mm(2), respectively. These data indicate that this antigen retrieval method is able to make immunoreactive GLP-2 available for detection and that GLP-2 may act as one of the common hormones secreted by L cells in the chicken small intestine.
authors: Department of Bioscience and Food Production Science, Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304 Minami-minowa, Kami-ina, Nagano 399-4598, Japan.
authors: Mohammad M
authors: MM
authors: Monir
authors: Kohzy
authors: K
authors: Hiramatsu
authors: Kei
authors: K
authors: Nishimura
authors: Chihiro
authors: C
authors: Takemoto
authors: Takafumi
authors: T
authors: Watanabe
mesh_terms: D000818:Animals; D002645:Chickens / Q000378:metabolism*; D002949:Citraconic Anhydrides; D019858:Enteroendocrine Cells / Q000276:immunology / Q000378:metabolism*; D053767:Glucagon-Like Peptide 2 / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary*; D007421:Intestine, Small / Q000378:metabolism*; D008297:Male
chemical_list: D002949:Citraconic Anhydrides; D053767:Glucagon-Like Peptide 2