title: Accounting for population variation in targeted proteomics.
abstract: Individual proteomes typically differ from the reference human proteome at ∼10,000 single amino acid variants. When viewed on the population scale, this individual variation results in a wide variety of protein sequences. In targeted proteomics experiments, such variability can confound accurate protein quantification. To assist researchers in identifying target peptides with high variability within the human population, we have created the Population Variation plug-in for Skyline, which provides easy access to the polymorphisms stored in dbSNP. Given a set of peptides, the tool reports minor allele frequency for common polymorphisms. We highlight the importance of considering genetic variation by applying the tool to public data sets.
authors: Biological Sciences Division, Pacific Northwest National Laboratory , 902 Battelle Boulevard, Richland, Washington 99532, United States.
authors: Grant M
authors: GM
authors: Fujimoto
authors: Matthew E
authors: ME
authors: Monroe
authors: Larissa
authors: L
authors: Rodriguez
authors: Chaochao
authors: C
authors: Wu
authors: Brendan
authors: B
authors: MacLean
authors: Richard D
authors: RD
authors: Smith
authors: Michael J
authors: MJ
authors: MacCoss
authors: Samuel H
authors: SH
authors: Payne
mesh_terms: D006801:Humans; D011110:Polymorphism, Genetic; D040901:Proteomics