title: Studies on the expression of linalool synthase using a promoter-β-glucuronidase fusion in transgenic Artemisia annua.
abstract: Artemisinin, an antimalarial endoperoxide sesquiterpene, is synthesized in glandular trichomes of Artemisia annua L. A number of other enzymes of terpene metabolism utilize intermediates of artemisinin biosynthesis, such as isopentenyl and farnesyl diphosphate, and may thereby influence the yield of artemisinin. In order to study the expression of such enzymes, we have cloned the promoter regions of some enzymes and fused them to β-glucuronidase (GUS). In this study, we have investigated the expression of the monoterpene synthase linalool synthase (LIS) using transgenic A. annua carrying the GUS gene under the control of the LIS promoter. The 652bp promoter region was cloned by the genome walker method. A number of putative cis-acting elements were predicted indicating that the LIS is driven by a complex regulation mechanism. Transgenic plants carrying the promoter-GUS fusion showed specific expression of GUS in T-shaped trichomes (TSTs) but not in glandular secretory trichomes, which is the site for artemisinin biosynthesis. GUS expression was observed at late stage of flower development in styles of florets and in TSTs and guard cells of basal bracts. GUS expression after wounding showed that LIS is involved in plant responsiveness to wounding. Furthermore, the LIS promoter responded to methyl jasmonate (MeJA). These results indicate that the promoter carries a number of cis-acting regulatory elements involved in the tissue-specific expression of LIS and in the response of the plant to wounding and MeJA treatment. Southern blot analysis indicated that the GUS gene was integrated in the A. annua genome as single or multi copies in different transgenic lines. Promoter activity analysis by qPCR showed that both the wild-type and the recombinant promoter are active in the aerial parts of the plant while only the recombinant promoter was active in roots. Due to the expression in TSTs but not in glandular trichomes, it may be concluded that LIS expression will most likely have little or no effect on artemisinin production.
authors: Department of Chemistry and Biomedical Sciences, Linnaeus University, Kalmar, Sweden.
authors: Hongzhen
authors: H
authors: Wang
authors: Department of Chemistry and Biomedical Sciences, Linnaeus University, Kalmar, Sweden.
authors: Selvaraju
authors: S
authors: Kanagarajan
authors: Department of Chemistry and Biomedical Sciences, Linnaeus University, Kalmar, Sweden.
authors: Junli
authors: J
authors: Han
authors: Department of Chemistry and Biomedical Sciences, Linnaeus University, Kalmar, Sweden.
authors: Mengshu
authors: M
authors: Hao
authors: Department of Chemistry and Biomedical Sciences, Linnaeus University, Kalmar, Sweden.
authors: Yiyi
authors: Y
authors: Yang
authors: Department of Chemistry and Biomedical Sciences, Linnaeus University, Kalmar, Sweden.
authors: Anneli
authors: A
authors: Lundgren
authors: Department of Chemistry and Biomedical Sciences, Linnaeus University, Kalmar, Sweden. Electronic address: peter.brodelius@lnu.se.
authors: Peter E
authors: PE
authors: Brodelius
mesh_terms: D000085:Acetates; D031172:Artemisia annua / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D003517:Cyclopentanes; D005966:Glucuronidase / Q000235:genetics; D006836:Hydro-Lyases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D054883:Oxylipins; D030821:Plants, Genetically Modified / Q000378:metabolism; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014170:Transformation, Genetic
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; C072239:methyl jasmonate; D005966:Glucuronidase; D006836:Hydro-Lyases; C092055:S-linalool synthase
keywords: ACT; ADS; ALDH1; Artemisinin biosynthesis; CYP71AV1; DBR2; ECS; FS; GAS; GST; GUS; LIS; Linalool synthase; MeJA; Promoter activity; SQS; T-shaped trichome; T-shaped trichomes; TST; aldehyde dehydrogenase 1; amorpha-4,11-diene 12-hydroxylase; amorpha-4,11-diene synthase; artemisinic aldehyde Δ11(13) reductase; artemisinin-based combination therapy; cis-Acting regulatory elements; epi-cedrol synthase; farnesene synthase; germacrene A synthase; glandular secretory trichome; linalool synthase; methyl jasmonate; squalene synthase; β-CPS; β-caryophyllene synthase; β-glucuronidase