title: Antibiotic resistances of intestinal lactobacilli isolated from wild boars.
abstract: Acquired antibiotic resistances have been reported in lactobacilli of various animal and food sources, but there are no data from wild boar. The objective was a preliminary examination of the antibiotic resistance prevalence of intrinsically vancomycin-resistant lactobacilli isolated from wild boar intestines and analysis of the genetic determinants implicated. Out of three wild boars, 121 lactobacilli were recovered and grouped according to their whole cell protein patterns. Initial phenotypic screening revealed that all were susceptible to erythromycin (2 μg/ml), but 30 were resistant to tetracycline (32 μg/ml). Based on Randomly Amplified Polymorphic DNA-PCR clustering, 64 strains were selected as representative genotypes for identification and minimum inhibitory concentration (MIC) determination. Partial 16S rRNA gene sequencing identified four species: (i) L. mucosae (n=57), (ii) L. reuteri (n=47), (iii) L. fermentum (n=12), and (iv) L. murinus (n=5). Most heterofermentative strains displayed low MICs for ampicillin (AMP), chloramphenicol (CHL), streptomycin (STR), kanamycin (KAN), gentamicin (GEN), erythromycin (ERY), quinupristin/dalfopristin (Q/D), and clindamycin (CLI). Atypical MICs were found mainly in L. mucosae and L. reuteri for TET, KAN, STR, AMP and CHL, but except the TET MICs of L. mucosae mostly at low level. L. murinus strains revealed atypical MICs for aminoglycosides, and/or CHL, AMP, CLI. PCR screening detected tet(W) in 12 and tet(M) in one of heterofermentative strains, as well as the aph(3')-III kanamycin gene in L. murinus. This is the first report showing acquired antibiotic resistance determinants in intestinal lactobacilli of wild boar origin.
authors: BOKU - University of Natural Resources and Applied Life Sciences, Vienna, Dep. IFA-Tulln, Division Environmental Biotechnology, Konrad Lorenz Straße 20, 3430 Tulln, Austria. Electronic address: viviana.klose@boku.ac.at.
authors: Viviana
authors: V
authors: Klose
authors: BOKU - University of Natural Resources and Applied Life Sciences, Vienna, Dep. IFA-Tulln, Division Environmental Biotechnology, Konrad Lorenz Straße 20, 3430 Tulln, Austria.
authors: Katharina
authors: K
authors: Bayer
authors: BOKU - University of Natural Resources and Applied Life Sciences, Vienna, Dep. IFA-Tulln, Division Environmental Biotechnology, Konrad Lorenz Straße 20, 3430 Tulln, Austria.
authors: Corinna
authors: C
authors: Kern
authors: BIOMIN Research Center, Technopark 1, 3430 Tulln, Austria.
authors: Florian
authors: F
authors: Goelß
authors: BIOMIN Research Center, Technopark 1, 3430 Tulln, Austria.
authors: Silvia
authors: S
authors: Fibi
authors: BIOMIN Research Center, Technopark 1, 3430 Tulln, Austria.
authors: Gertrude
authors: G
authors: Wegl
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000235:genetics; D005838:Genotype; D007422:Intestines / Q000382:microbiology*; D007778:Lactobacillus / Q000187:drug effects* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D034421:Sus scrofa / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D012336:RNA, Ribosomal, 16S
keywords: Antibiotic resistances; Lactobacilli; Wild boar intestine