title: The effects of elastic fiber protein insufficiency and treatment on the modulus of arterial smooth muscle cells.
abstract: Elastic fibers are critical for the mechanical function of the large arteries. Mechanical effects of elastic fiber protein deficiency have been investigated in whole arteries, but not in isolated smooth muscle cells (SMCs). The elastic moduli of SMCs from elastin (Eln-/-) and fibulin-4 (Fbln4-/-) knockout mice were measured using atomic force microscopy. Compared to control SMCs, the modulus of Eln-/- SMCs is reduced by 40%, but is unchanged in Fbln4-/- SMCs. The Eln-/- SMC modulus is rescued by soluble or α elastin treatment. Altered gene expression, specifically of calponin, suggests that SMC phenotypic modulation may be responsible for the modulus changes.
authors: M
authors: M
authors: Gabriela Espinosa
authors: William S
authors: WS
authors: Gardner
authors: Lisa
authors: L
authors: Bennett
authors: Bradley A
authors: BA
authors: Sather
authors: Hiromi
authors: H
authors: Yanagisawa
authors: Jessica E
authors: JE
authors: Wagenseil
mesh_terms: D000818:Animals; D001158:Arteries / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D055119:Elastic Modulus / Q000187:drug effects / Q000502:physiology; D004547:Elastic Tissue / Q000187:drug effects / Q000502:physiology*; D004549:Elastin / Q000494:pharmacology / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000502:physiology*
chemical_list: C403672:EFEMP2 protein, human; D016326:Extracellular Matrix Proteins; D004549:Elastin