title: Differential effects of eicosapentaenoic acid and docosahexaenoic acid in promoting the differentiation of 3T3-L1 preadipocytes.
abstract: The objective of this study was to determine the effects of enrichment with n-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), on the differentiation of 3T3-L1 preadipocytes. Enrichment with DHA but not EPA significantly increased the differentiation markers compared to control differentiated cells. DHA compared to EPA treatment led to a greater increase in adiponectin secretion and, conditioned media collected from DHA treated cells inhibited monocyte migration. Moreover, DHA treatment resulted in inhibition of pro-inflammatory signaling pathways. DHA treated cells predominantly accumulated DHA in phospholipids whereas EPA treatment led to accumulation of both EPA and its elongation product docosapentaenoic acid (DPA), an n-3 fatty acid. Of note, adding DPA to DHA inhibited DHA-induced differentiation. The differential effects of EPA and DHA on preadipocyte differentiation may be due, in part, to differences in their intracellular modification which could impact the type of n-3 fatty acids incorporated into the cells.
authors: Departments of Internal Medicine, Division of Diabetes, Endocrinology, and Metabolism, Omaha, NE, United States; Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE, United States; Research Services, VA Nebraska Western Iowa Health Care System, Omaha, NE, United States.
authors: Ganesan
authors: G
authors: Murali
authors: Research Services, VA Nebraska Western Iowa Health Care System, Omaha, NE, United States; Departments of Internal Medicine, Division of Diabetes, Endocrinology, and Metabolism, Omaha, NE, United States.
authors: Cyrus V
authors: CV
authors: Desouza
authors: Departments of Internal Medicine, Division of Diabetes, Endocrinology, and Metabolism, Omaha, NE, United States; Research Services, VA Nebraska Western Iowa Health Care System, Omaha, NE, United States.
authors: Michelle E
authors: ME
authors: Clevenger
authors: Departments of Internal Medicine, Division of Diabetes, Endocrinology, and Metabolism, Omaha, NE, United States; Research Services, VA Nebraska Western Iowa Health Care System, Omaha, NE, United States.
authors: Ramesh
authors: R
authors: Ramalingam
authors: Departments of Internal Medicine, Division of Diabetes, Endocrinology, and Metabolism, Omaha, NE, United States; Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE, United States; Research Services, VA Nebraska Western Iowa Health Care System, Omaha, NE, United States. Electronic address: s.viswanathan@unmc.edu.
authors: Viswanathan
authors: V
authors: Saraswathi
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D051379:Mice
chemical_list: D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid
keywords: Adipogenesis; DHA; Docosapentaenoic acid; EPA; n-3 fatty acids