title: Human neural stem cells expressing carboxyl esterase target and inhibit tumor growth of lung cancer brain metastases.
abstract: Neural stem cells (NSCs) led to the development of a novel strategy for delivering therapeutic genes to brain tumors. Human NSCs expressing rabbit carboxyl esterase (F3.CE), which activates CPT-11, significantly inhibit the growth of A549 human non-small cell lung adenocarcinoma cells in the presence of CPT-11 in vitro and in vivo. F3.CE cells migrated selectively into the brain metastases located in the opposite hemisphere. The treatment also significantly decreased tumor volume in immune-deficient mice bearing lung cancer when F3.CE cells were transplanted into the contralateral hemisphere. The survival of tumor-bearing animals was significantly prolonged by the treatment with F3.CE and CPT-11. This strategy could be considered as an effective treatment regimen for lung cancer brain metastases.
authors: Department of Neurosurgery, Ulsan University College of Medicine Asan Medical Center, Seoul, Korea.
authors: S H
authors: SH
authors: Hong
authors: Medical Research Institute, Chung-Ang University College of Medicine, Seoul, Korea.
authors: H J
authors: HJ
authors: Lee
authors: Medical Research Institute, Chung-Ang University College of Medicine, Seoul, Korea.
authors: J
authors: J
authors: An
authors: Department of Physiology, Chung-Ang University College of Medicine, Seoul, Korea.
authors: I
authors: I
authors: Lim
authors: Department of Neurosurgery, University of South Florida College of Medicine, Tampa, FL, USA.
authors: C
authors: C
authors: Borlongan
authors: Department of Neurosciences, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA, USA.
authors: K S
authors: KS
authors: Aboody
authors: 1] Medical Research Institute, Chung-Ang University College of Medicine, Seoul, Korea [2] Division of Neurology, Department of Medicine, UBC Hospital, University of British Columbia, Vancouver, BC, Canada.
authors: S U
authors: SU
authors: Kim
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000556:secondary*; D024201:Bystander Effect; D043182:Carboxylesterase / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D004195:Disease Models, Animal; D015870:Gene Expression; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology*; D058953:Neural Stem Cells / Q000378:metabolism*; D011817:Rabbits; D047368:Tumor Burden / Q000235:genetics
chemical_list: D043182:Carboxylesterase