title: A mitochondrial location for haemoglobins--dynamic distribution in ageing and Parkinson's disease.
abstract: Haemoglobins are iron-containing proteins that transport oxygen in the blood of most vertebrates. The mitochondrion is the cellular organelle which consumes oxygen in order to synthesise ATP. Mitochondrial dysfunction is implicated in neurodegeneration and ageing. We find that α and β haemoglobin (Hba and Hbb) proteins are altered in their distribution in mitochondrial fractions from degenerating brain. We demonstrate that both Hba and Hbb are co-localised with the mitochondrion in mammalian brain. The precise localisation of the Hbs is within the inner membrane space and associated with inner mitochondrial membrane. Relative mitochondrial to cytoplasmic ratios of Hba and Hbb show changing distributions of these proteins during the process of neurodegeneration in the pcd(5j) mouse brain. A significant difference in mitochondrial Hba and Hbb content in the mitochondrial fraction is seen at 31 days after birth, this corresponds to a stage when dynamic neuronal loss is measured to be greatest in the Purkinje Cell Degeneration mouse. We also report changes in mitochondrial Hba and Hbb levels in ageing brain and muscle. Significant differences in mitochondrial Hba and Hbb can be seen when comparing aged brain to muscle, suggesting tissue specific functions of these proteins in the mitochondrion. In muscle there are significant differences between Hba levels in old and young mitochondria. To understand whether the changes detected in mitochondrial Hbs are of clinical significance, we examined Parkinson's disease brain, immunohistochemistry studies suggest that cell bodies in the substantia nigra accumulate mitochondrial Hb. However, western blotting of mitochondrial fractions from PD and control brains indicates significantly less Hb in PD brain mitochondria. One explanation could be a specific loss of cells containing mitochondria loaded with Hb proteins. Our study opens the door to an examination of the role of Hb function, within the context of the mitochondrion-in health and disease.
authors: School of Veterinary Medicine and Science, Faculty of Medicine and Health Sciences, University of Nottingham, LE12 5RD, United Kingdom.
authors: Freya
authors: F
authors: Shephard
authors: School of Veterinary Medicine and Science, Faculty of Medicine and Health Sciences, University of Nottingham, LE12 5RD, United Kingdom.
authors: Oliver
authors: O
authors: Greville-Heygate
authors: School of Veterinary Medicine and Science, Faculty of Medicine and Health Sciences, University of Nottingham, LE12 5RD, United Kingdom.
authors: Oliver
authors: O
authors: Marsh
authors: Graduate Entry Medicine, University of Nottingham, DE22 3DT, United Kingdom.
authors: Susan
authors: S
authors: Anderson
authors: School of Veterinary Medicine and Science, Faculty of Medicine and Health Sciences, University of Nottingham, LE12 5RD, United Kingdom. Electronic address: lisa.chakrabarti@nottingham.ac.uk.
authors: Lisa
authors: L
authors: Chakrabarti
mesh_terms: D000375:Aging; D000818:Animals; D001921:Brain / Q000473:pathology; D003593:Cytoplasm / Q000737:chemistry; D006441:Hemoglobin A / Q000032:analysis*; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D051379:Mice; D008928:Mitochondria / Q000737:chemistry*; D009132:Muscles / Q000473:pathology; D010300:Parkinson Disease / Q000473:pathology*
chemical_list: D006454:Hemoglobins; D006441:Hemoglobin A; C020622:hemoglobin B
keywords: Ageing; Haemoglobin; Mitochondria; Neurodegeneration; Parkinson's Disease