title: Comparison of in Vitro Activity of Doripenem versus Old Carbapenems against Pseudomonas Aeruginosa Clinical Isolates from both CF and Burn Patients.
abstract: OBJECTIVE
  The antimicrobial activity of doripenem in comparison of imipenem, meropenem and ertapenem among Pseudomonas aeruginosa isolated from burn and Cystic Fibrosis (CF) patients were determined.
  
  
  METHODS
  Metallo-β-lactamase (MBL) genes in imipenem non susceptible P. aeruginosa isolates were detected using PCR method. The in vitro susceptibilities of doripenem, imipenem, meropenem and ertapenem were determined by Etests. MIC50 and MIC90 for corresponding antibiotics were determined individually in burn and CF isolates.
  
  
  RESULTS
  Among isolates which were resistant to imipenem, 16 isolates were positive for the bla IMP gene. All isolates had no bla VIM gene. All MBL producing isolates were excluded. MIC50/MIC90 of doripenem in CF and burn isolates were 0.75/>32 and >32/>32 mg/L respectively. The corresponding values for imipenem in CF and burn isolates were 2/>32 and >32/>32 mg/L, respectively.
  
  
  CONCLUSIONS
  The susceptibility rate of doripenem is higher than that of imipenem and meropenem among P.aeruginosa isolated from CF patients, whereas, there is no difference between the efficiency of doripenem and old carbapenems in non MBL producing P.aeruginosa isolates in burn patients.
authors: Tabriz Research Center of Infectious and Tropical Diseases, Tabriz University of Medical Sciences, Tabriz, Iran. ; Microbiology Department, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Zoya
authors: Z
authors: Hojabri
authors: Mohammad
authors: M
authors: Ahangarzadeh Rezaee
authors: Mohammad Reza
authors: MR
authors: Nahaei
authors: Mohammad Hossein
authors: MH
authors: Soroush
authors: Morteza
authors: M
authors: Ghojazadeh
authors: Tahereh
authors: T
authors: Pirzadeh
authors: Mostafa
authors: M
authors: Davodi
authors: Mona
authors: M
authors: Ghazi
authors: Reza
authors: R
authors: Bigverdi
authors: Omid
authors: O
authors: Pajand
authors: Mohammad
authors: M
authors: Aghazadeh
keywords: Burn; Cystic fibrosis; Doripenem; Imipenem; Iran; Pseudomonas aeruginosa