title: Hair growth-promoting effect of Carthamus tinctorius floret extract.
abstract: The florets of Carthamus tinctorius L. have traditionally been used for hair growth promotion. This study aimed to examine the potential of hydroxysafflor yellow A-rich C. tinctorius extract (CTE) on hair growth both in vitro and in vivo. The effect of CTE on cell proliferation and hair growth-associated gene expression in dermal papilla cells and keratinocytes (HaCaT) was determined. In addition, hair follicles from mouse neonates were isolated and cultured in media supplemented with CTE. Moreover, CTE was applied topically on the hair-shaved skin of female C57BL/6 mice, and the histological profile of the skin was investigated. C. tinctorius floret ethanolic extract promoted the proliferation of both dermal papilla cells and HaCaT and significantly stimulated hair growth-promoting genes, including vascular endothelial growth factor and keratinocyte growth factor. In contrast, CTE suppressed the expression of transforming growth factor-β1 that is the hair loss-related gene. Furthermore, CTE treatment resulted in a significant increase in the length of cultured hair follicles and stimulated the growth of hair with local effects in mice. The results provided the preclinical data to support the potential use of CTE as a hair growth-promoting agent.
authors: Center for Research and Development of Herbal Health Products, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.
authors: Jintana
authors: J
authors: Junlatat
authors: Bungorn
authors: B
authors: Sripanidkulchai
mesh_terms: D000818:Animals; D031187:Carthamus tinctorius / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D020405:Dermis / Q000166:cytology; D005260:Female; D051523:Fibroblast Growth Factor 7 / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006197:Hair / Q000254:growth & development*; D018859:Hair Follicle / Q000187:drug effects*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; D051523:Fibroblast Growth Factor 7
keywords: Carthamus tinctorius; hair growth; keratinocyte growth factor; transforming growth factor-β1; vascular endothelial growth factor