title: Role of the nuclease activities encoded by herpes simplex virus 1 UL12 in viral replication and neurovirulence.
abstract: Enzyme-dead mutations in the herpes simplex virus 1 UL12 gene that abolished its endo- and exonuclease activities only slightly reduced viral replication in cell cultures. However, the UL12 null mutation significantly reduced viral replication, suggesting that a UL12 function(s) unrelated to its nuclease activities played a major role in viral replication. In contrast, the enzyme-dead mutations significantly reduced viral neurovirulence in mice, suggesting that UL12 nuclease activities were critical for viral pathogenesis in vivo.
authors: Division of Molecular Virology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
authors: Hikaru
authors: H
authors: Fujii
authors: Michio
authors: M
authors: Mugitani
authors: Naoto
authors: N
authors: Koyanagi
authors: Zhuoming
authors: Z
authors: Liu
authors: Shumpei
authors: S
authors: Tsuda
authors: Jun
authors: J
authors: Arii
authors: Akihisa
authors: A
authors: Kato
authors: Yasushi
authors: Y
authors: Kawaguchi
mesh_terms: D017931:DNA Primers / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D003851:Deoxyribonucleases / Q000378:metabolism / Q000502:physiology*; D018259:Herpesvirus 1, Human / Q000201:enzymology* / Q000472:pathogenicity*; D009154:Mutation / Q000235:genetics; D014764:Viral Proteins / Q000378:metabolism / Q000502:physiology*; D014774:Virulence; D014779:Virus Replication / Q000502:physiology*
chemical_list: D017931:DNA Primers; D004274:DNA, Recombinant; D014764:Viral Proteins; D003851:Deoxyribonucleases; C539238:UL12 protein, herpes simplex virus 1