title: Beneficial effect of resveratrol on bovine oocyte maturation and subsequent embryonic development after in vitro fertilization.
abstract: OBJECTIVE
  To analyze the potential beneficial effects and mechanisms of action of resveratrol on the maturation of bovine oocytes that were incubated in different concentrations of resveratrol (0.1, 1.0, or 10.0 μM) as germinal vesicle-stage oocytes.
  
  
  METHODS
  In vitro prospective study.
  
  
  METHODS
  University research laboratory.
  
  
  METHODS
  Animal models for human studies.
  
  
  METHODS
  In vitro culture in the presence of various concentrations of the antioxidant resveratrol.
  
  
  METHODS
  Parameters of hormone levels, oocyte nuclear maturation, cumulus expansion, levels of intracellular glutathione and reactive oxygen species, embryonic cleavage, blastocyst formation, gene expression associated with mature bovine oocytes and cumulus cells, and level of sirtuin 1 gene expression.
  
  
  RESULTS
  Resveratrol statistically significantly increased progesterone secretion and decreased estradiol-17β secretion by cumulus cells. The elevated levels of progesterone activated the Mos/MEK/p42 mitogen-activated protein kinase (MAPK) cascade in the oocytes. At a concentration of 1.0 μM, resveratrol statistically significantly improved cumulus expansion, polar body formation, the (hatched) blastocyst rate, and the mean number of cells/blastocysts. Meanwhile, resveratrol statistically significantly reduced the level of reactive oxygen species (ROS) and increased the level of glutathione (GSH). For the first time, the expression of the sirtuin-1 gene was identified in granulosa cells, cumulus cells, oocytes, and blastocysts. Further studies revealed that resveratrol promoted sirtuin-1 gene expression.
  
  
  CONCLUSIONS
  Resveratrol promoted bovine oocyte maturation and subsequent post-in vitro fertilization embryonic development by inducing progesterone secretion and an antioxidant effect, probably in a manner dependent on sirtuin-1.
authors: State Key Laboratory of Animal Nutrition, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China.
authors: Feng
authors: F
authors: Wang
authors: State Key Laboratory of Animal Nutrition, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China.
authors: XiuZhi
authors: X
authors: Tian
authors: State Key Laboratory of Animal Nutrition, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China.
authors: Lu
authors: L
authors: Zhang
authors: State Key Laboratory of Animal Nutrition, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China.
authors: ChangJiu
authors: C
authors: He
authors: State Key Laboratory of Animal Nutrition, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China.
authors: PengYun
authors: P
authors: Ji
authors: State Key Laboratory of Animal Nutrition, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China.
authors: Yu
authors: Y
authors: Li
authors: Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, Texas.
authors: DunXian
authors: D
authors: Tan
authors: State Key Laboratory of Animal Nutrition, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China. Electronic address: gshliu@cau.edu.cn.
authors: GuoShi
authors: G
authors: Liu
mesh_terms: D000818:Animals; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D047108:Embryonic Development / Q000187:drug effects* / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development; D011247:Pregnancy; D011446:Prospective Studies; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D016896:Treatment Outcome
chemical_list: D013267:Stilbenes; D000077185:Resveratrol
keywords: Antioxidant; SIRT1; bovine; oocyte maturation; resveratrol