title: Effects of ethanol extract of Radix Sophorae Flavescentis on activity of colon cancer HT29 cells.
abstract: This paper mainly studied the inhibitory effect of total ethanol extract of Radix Sophorae Flavescentis on proliferation of colon cancer HT29 cells. By reflux extraction method and with ethanol as extraction solvent, different extracts were obtained at different ethanol concentrations, different solid-liquid ratios, and at different times. And their inhibitory activities against HT29 cells were compared using MTT assay. The experimental results showed that the extraction processes under three conditions can all draw relatively high inhibition rates. The optimum ethanol extraction process conditions were as follows: a solid-liquid ratio of 1:9, 80 min of heat reflux extraction with 95% ethanol.
authors: Department of Gastroenterology, the third hospital of Xiangya, Central South University, Changsha, Hunan 410013, China.
authors: Zhi-Ming
authors: ZM
authors: Xiao
authors: Department of Emergency, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
authors: Ai-Min
authors: AM
authors: Wang
authors: Department of Gastroenterology, the third hospital of Xiangya, Central South University, Changsha, Hunan 410013, China.
authors: Xiao-Yan
authors: XY
authors: Wang
authors: Department of Gastroenterology, the third hospital of Xiangya, Central South University, Changsha, Hunan 410013, China.
authors: Shou-Rong
authors: SR
authors: Shen
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use*; D003110:Colonic Neoplasms / Q000188:drug therapy*; D019073:HT29 Cells; D006801:Humans; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018517:Plant Roots; D029909:Sophora
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts
keywords: Orthogonal experiment; Sophora flavescens; cancer HT29; inhibition