title: Fluorescence enhancement of quercetin complexes by silver nanoparticles and its analytical application.
abstract: It is found that the plasmon effect of silver nanoparticles (AgNPs) helps to enhance the fluorescence intensity of the quercetin (Qu) and nucleic acids system. Qu exhibited strong fluorescence enhancement when it bound to nucleic acids in the presence of AgNPs. Based on this, a sensitive method for the determination of nucleic acids was developed. The detection limits for the nucleic acids (S/N=3) were reduced to the ng mL(-1) level. The interaction mechanism of the AgNPs-fish sperm DNA (fsDNA)-Qu system was also investigated in this paper. This complex system of Qu and AgNPs was also successfully used for the detection of nucleic acids in agarose gel electrophoresis analysis. Preliminary results indicated that AgNPs also helped to improve sensitivity in the fluorescence image analysis of Qu combined with cellular contents in Arabidopsis thaliana protoplasts.
authors: Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors: Ping
authors: P
authors: Liu
authors: Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors: Liangliang
authors: L
authors: Zhao
authors: Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China. Electronic address: wux@sdu.edu.cn.
authors: Xia
authors: X
authors: Wu
authors: Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors: Fei
authors: F
authors: Huang
authors: School of Life Sciences, Shandong University, Jinan 250100, PR China.
authors: Minqin
authors: M
authors: Wang
authors: Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors: Xiaodan
authors: X
authors: Liu
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000166:cytology; D004247:DNA / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D005399:Fishes; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009690:Nucleic Acid Conformation; D011523:Protoplasts / Q000166:cytology; D011794:Quercetin / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D012834:Silver; D004247:DNA; D011794:Quercetin
keywords: Fluorescence enhancement; Nucleic acids; Quercetin; Silver nanoparticles