title: [Regulation effect of adipose-derived mesenchymal stem cells on pancreatic cancer cells].
abstract: OBJECTIVE
  To analyze the effects of adipose tissue-derived stem cells (ADSCs) on the proliferation and invasion of pancreatic cancer (PaCa) cells and the the possible mechanism involved.
  
  
  METHODS
  ADSCs were isolated and co-cultured with PaCa cells. CCK-8 assay was used to detect the proliferation of PaCa cells. An ELISA was used to determine the concentration of stromal cell-derived factor-1 (SDF-1) in the supernatants. The proliferation of PaCa cells by SDF-1 was measured. AMD3100 regulated the co-culture of ADSCs and PaCa. The tumor growth of PaCa cells was assessed after treatment by ADSCs in vivo.
  
  
  RESULTS
  ADSCs can promote the proliferation and invasion of PaCa cells (proliferation: SW1990: 1.535 ± 0.153; PANC-1: 1.370 ± 0.100; the value of control was 1; invasion: SW1990: 47.0 ± 2.6 vs. 28.3 ± 1.3; PANC-1: 40.3 ± 1.8 vs. 24.3 ± 1.3; t = 4.332-9.558, P < 0.05). The expression of SDF-1 was high in ADSCs, but not in PaCa cells (69 ± 5 vs. 0 and 0, F = 389.134, P < 0.01). The promotion of SDF-1 on PaCa cells depends on the concentration. AMD3100 significantly downregulates these growth-promoting effects of ADSCs on PaCa cells. ADSCs significantly promoted the growth of SW1990 in nude mice at the 5(th) week (volume: (1295 ± 102) mm(3) vs. (967 ± 81) mm(3), t = 5.614, P < 0.05) , but not in PANC-1 cell.
  
  
  CONCLUSIONS
  ADSCs can promote the proliferation and invasion of PaCa cells, which may involve the SDF-1/CXCR4 axis.
authors: Department of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China.
authors: Fu-Xiang
authors: FX
authors: Yu
authors: Department of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China.
authors: Shi-Qiang
authors: SQ
authors: Ji
authors: Department of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China.
authors: Jun-Qiao
authors: JQ
authors: Zhong
authors: Department of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China.
authors: Qian-Dong
authors: QD
authors: Zhu
authors: Department of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China.
authors: Yang-Yang
authors: YY
authors: Li
authors: Department of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China. Email: zqy80@163.com.
authors: Qi-Yu
authors: QY
authors: Zhang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D059630:Mesenchymal Stem Cells; D008819:Mice, Nude; D010190:Pancreatic Neoplasms