title: Inactivation of genes coding for mitochondrial Nd7 and Nd9 complex I subunits in Chlamydomonas reinhardtii. Impact of complex I loss on respiration and energetic metabolism.
abstract: In Chlamydomonas, unlike in flowering plants, genes coding for Nd7 (NAD7/49 kDa) and Nd9 (NAD9/30 kDa) core subunits of mitochondrial respiratory-chain complex I are nucleus-encoded. Both genes possess all the features that facilitate their expression and proper import of the polypeptides in mitochondria. By inactivating their expression by RNA interference or insertional mutagenesis, we show that both subunits are required for complex I assembly and activity. Inactivation of complex I impairs the cell growth rate, reduces the respiratory rate, leads to lower intracellular ROS production and lower expression of ROS scavenging enzymes, and is associated to a diminished capacity to concentrate CO2 without compromising photosynthetic capacity.
authors: Genetics of Microorganisms, PhytoSYSTEMS, Department of Life Sciences, University of Liège, Belgium.
authors: Simon
authors: S
authors: Massoz
authors: Genetics of Microorganisms, PhytoSYSTEMS, Department of Life Sciences, University of Liège, Belgium.
authors: Véronique
authors: V
authors: Larosa
authors: Genetics of Microorganisms, PhytoSYSTEMS, Department of Life Sciences, University of Liège, Belgium.
authors: Charlotte
authors: C
authors: Plancke
authors: Genetics of Microorganisms, PhytoSYSTEMS, Department of Life Sciences, University of Liège, Belgium.
authors: Marie
authors: M
authors: Lapaille
authors: Genetics of Microorganisms, PhytoSYSTEMS, Department of Life Sciences, University of Liège, Belgium.
authors: Benjamin
authors: B
authors: Bailleul
authors: Genetics of Microorganisms, PhytoSYSTEMS, Department of Life Sciences, University of Liège, Belgium.
authors: Dorothée
authors: D
authors: Pirotte
authors: Genetics of Microorganisms, PhytoSYSTEMS, Department of Life Sciences, University of Liège, Belgium.
authors: Michèle
authors: M
authors: Radoux
authors: GIGA-Neuroscience, University of Liège, Belgium.
authors: Pierre
authors: P
authors: Leprince
authors: Genetics of Microorganisms, PhytoSYSTEMS, Department of Life Sciences, University of Liège, Belgium.
authors: Nadine
authors: N
authors: Coosemans
authors: Genetics of Microorganisms, PhytoSYSTEMS, Department of Life Sciences, University of Liège, Belgium.
authors: René F
authors: RF
authors: Matagne
authors: Genetics of Microorganisms, PhytoSYSTEMS, Department of Life Sciences, University of Liège, Belgium.
authors: Claire
authors: C
authors: Remacle
authors: Genetics of Microorganisms, PhytoSYSTEMS, Department of Life Sciences, University of Liège, Belgium. Electronic address: pierre.cardol@ulg.ac.be.
authors: Pierre
authors: P
authors: Cardol
mesh_terms: D019069:Cell Respiration; D016825:Chlamydomonas reinhardtii / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D004734:Energy Metabolism; D055785:Gene Knockdown Techniques; D055786:Gene Knockout Techniques; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism
chemical_list: D024101:Mitochondrial Proteins; D010940:Plant Proteins; D021122:Protein Subunits; D009245:NADH Dehydrogenase
keywords: Chlamydomonas; Energetic metabolism; NAD7/NDUFS2; NAD9/NDUFS3; NADH:ubiquinone oxidoreductase; Oxidative stress