title: Spinal synaptic scaffolding protein Homer 1b/c regulates CREB phosphorylation and c-fos activation induced by inflammatory pain in rats.
abstract: Previous studies have shown that spinal Homer 1b/c plays an important role in the maintenance of chronic inflammatory pain induced by complete Freund's adjuvant (CFA). This study investigated the possible mechanism underlying Homer 1b/c mediating CFA-induced inflammatory pain. Chronic inflammation was induced by CFA injection into the left hind ankle of the rat. Homer 1b/c antisense or missense oligonucleotides were administered intrathecally (10μg/10μl) from 5 to 8 days following the onset of inflammation. Immunohistochemistry was conducted to detect the expression of phosphorylated cAMP response element binding protein (pCREB) and Fos protein in the spinal dorsal horn. Intrathecal administration of Homer 1b/c antisense oligonucleotides not only markedly reduced the expression of Homer 1b/c protein, but also attenuated CFA-induced inflammation, spinal CREB phosphorylation, and Fos expression. These results demonstrate for the first time that Homer 1b/c regulates CREB phosphorylation and c-fos activation in the spinal dorsal horn during the maintenance of chronic inflammatory pain, suggesting that Homer 1b/c may be involved in the development of CFA-induced inflammation.
authors: Department of Anesthesia, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
authors: Yong-Xing
authors: YX
authors: Yao
authors: Department of Anesthesia, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
authors: Yan-Feng
authors: YF
authors: Zhang
authors: Institute of Neurobiology, Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, China.
authors: Yan
authors: Y
authors: Yang
authors: Department of Anesthesia, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
authors: Shao-Hui
authors: SH
authors: Guo
authors: Department of Anesthesia, Zhongshan Hospital, Fudan University, Shanghai, China.
authors: Zhen
authors: Z
authors: Jiang
authors: Institute of Neurobiology, Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, China. Electronic address: zqzhao@fudan.edu.cn.
authors: Zhi-Qi
authors: ZQ
authors: Zhao
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000008:administration & dosage* / Q000502:physiology; D059350:Chronic Pain; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D016762:Genes, fos / Q000187:drug effects / Q000502:physiology*; D000072233:Homer Scaffolding Proteins; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D007278:Injections, Spinal; D008297:Male; D010146:Pain / Q000378:metabolism* / Q000473:pathology; D010766:Phosphorylation / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D013569:Synapses / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology
chemical_list: D002352:Carrier Proteins; D017362:Cyclic AMP Response Element-Binding Protein; D000072233:Homer Scaffolding Proteins
keywords: CCI; CFA; CREB; Fos; Homer 1b/c; Inflammatory pain; cAMP response element binding protein; chronic constriction injury; complete Freund's adjuvant; mGluRs; metabotropic glutamate receptors