title: Proteomic analysis of mismatch repair-mediated alkylating agent-induced DNA damage response.
abstract: BACKGROUND
  Mediating DNA damage-induced apoptosis is an important genome-maintenance function of the mismatch repair (MMR) system. Defects in MMR not only cause carcinogenesis, but also render cancer cells highly resistant to chemotherapeutics, including alkylating agents. To understand the mechanisms of MMR-mediated apoptosis and MMR-deficiency-caused drug resistance, we analyze a model alkylating agent (N-methyl-N'-nitro-N-nitrosoguanidine, MNNG)-induced changes in protein phosphorylation and abundance in two cell lines, the MMR-proficient TK6 and its derivative MMR-deficient MT1.
  
  
  RESULTS
  Under an experimental condition that MNNG-induced apoptosis was only observed in MutSa-proficient (TK6), but not in MutSa-deficient (MT1) cells, quantitative analysis of the proteomic data revealed differential expression and phosphorylation of numerous individual proteins and clusters of protein kinase substrates, as well differential activation of response pathways/networks in MNNG-treated TK6 and MT1 cells. Many alterations in TK6 cells are in favor of turning on the apoptotic machinery, while many of those in MT1 cells are to promote cell proliferation and anti-apoptosis.
  
  
  CONCLUSIONS
  Our work provides novel molecular insights into the mechanism of MMR-mediated DNA damage-induced apoptosis.
authors: Xi
authors: X
authors: Chen
authors: Yong
authors: Y
authors: Zhao
authors: Guo-Min
authors: GM
authors: Li
authors: Lin
authors: L
authors: Guo