title: Molecular diversity of poleroviruses infecting cucurbit crops in four countries reveals the presence of members of six distinct species.
abstract: When 66 cucurbit samples with yellowing symptoms from fields in Mali, the Philippines, Thailand and Uzbekistan were screened by RT-PCR using universal polerovirus primers, 21 were identified as harboring polerovirus RNA. When these 21 samples were screened with specific primers for the known cucurbit-infecting poleroviruses, suakwa aphid-borne yellows virus and a recombinant strain of cucurbit aphid-borne yellows virus were detected for the first time in the Philippines and Thailand. However, seven polerovirus-positive samples did not react with any of the known species-specific primers. Sequencing of 1.4-kb universal polerovirus RT-PCR products revealed the presence of two poleroviruses that had not been described previously. These viruses, from Mali and Thailand, were provisionally named pepo aphid-borne yellows virus and luffa aphid-borne yellows virus, respectively.
authors: AVRDC, The World Vegetable Center, PO Box 42, Shanhua, Tainan, 74199, Taiwan.
authors: D
authors: D
authors: Knierim
authors: W S
authors: WS
authors: Tsai
authors: E
authors: E
authors: Maiss
authors: L
authors: L
authors: Kenyon
mesh_terms: D001208:Asia; D016000:Cluster Analysis; D028464:Cucurbita / Q000821:virology*; D014644:Genetic Variation; D005838:Genotype; D052679:Luteoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral