title: Anatomical, physiological and transcriptional responses of two contrasting poplar genotypes to drought and re-watering.
abstract: Populus × euramericana (Pe) displays higher stable carbon isotope composition (δ(13)C) and intrinsic water use efficiency (WUEi) than Populus cathayana (Pc) under unlimited water conditions, rendering us to hypothesize that Pe is better acclimated to water deficiency than Pc. To examine this hypothesis, saplings of Pc and Pe were exposed to drought and subsequently re-watered. Pc and Pe exhibited distinct anatomical, physiological and transcriptional responses in acclimation to drought and re-watering, mainly due to stronger responsiveness of transcriptional regulation of genes encoding plasma membrane intrinsic proteins (PIPs), higher starch accumulation, δ(13)C, stable nitrogen isotope composition (δ(15)N) and WUEi , and lower reactive oxygen species (ROS) accumulation and scavenging in Pe. In acclimation to drought, both poplar genotypes demonstrated altered anatomical properties, declined height growth, differential expression of PIPs, activation of ABA signaling pathway, decreased total soluble sugars and starch, increased δ(13)C, δ(15)N and WUEi , and shifted homeostasis of ROS production and scavenging, and these changes can be recovered upon re-watering. These data indicate that Pe is more tolerant to drought than Pc, and that anatomical, physiological and transcriptional acclimation to drought and re-watering is essential for poplars to survive and grow under projected dry climate scenarios in the future.
authors: College of Life Sciences and State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, Shaanxi, 712100, China; Büsgen-Institute, Department of Forest Botany and Tree Physiology, Georg-August University, 37077, Göttingen, Germany.
authors: Xu
authors: X
authors: Cao
authors: Jingbo
authors: J
authors: Jia
authors: Chao
authors: C
authors: Zhang
authors: Hong
authors: H
authors: Li
authors: Tongxian
authors: T
authors: Liu
authors: Xiangning
authors: X
authors: Jiang
authors: Andrea
authors: A
authors: Polle
authors: Changhui
authors: C
authors: Peng
authors: Zhi-Bin
authors: ZB
authors: Luo
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002247:Carbon Isotopes; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D009587:Nitrogen Isotopes; D010788:Photosynthesis / Q000235:genetics; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D032107:Populus / Q000033:anatomy & histology* / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D025341:Principal Component Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012995:Solubility; D013402:Sugar Alcohols / Q000378:metabolism; D014158:Transcription, Genetic; D014867:Water / Q000502:physiology*
chemical_list: D000975:Antioxidants; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D010940:Plant Proteins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D013402:Sugar Alcohols; D014867:Water