title: [Comparison of ELISA diagnostic kits used in China for hepatitis C virus IgG antibody detection].
abstract: OBJECTIVE
  To compare the commercial diagnostic reagent available in China for hepatitis C virus ( HCV) IgG antibody detection in order to provide some useful information for HCV prevention.
  
  
  METHODS
  The HCV-IgG detection reagents produced by six different Enterprises named A, B, C, D, E and F were chosen and applied to detect 160 HCV specious sera samples. HCV-IgG reagent from ABBOTT was adopted as gold-standard and the samples in gray zone were determined by RIBA method finally.
  
  
  RESULTS
  160 sera samples comprised 88 positive samples and 72 negative samples. The total conformity ranged from 88.13% to 98.13% and the Youden indexes ranged from 0.74 to 0.96 when the reagents from six different Enterprises were compared with gold-standard. The highest conformity was 98.13%, observed in D reagent with the highest Youden index of 0.96.
  
  
  CONCLUSIONS
  The total conformity rates were more than 88% when the HCV-IgG antibody detection reagents from six different Enterprises were compared with ABBOTT reagent. It was highly conformable. However, some reagent proved to be less conformable in negative samples detection.
authors: Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
authors: Qing-Ling
authors: QL
authors: Meng
authors: Jian
authors: J
authors: Lu
authors: Feng
authors: F
authors: Qiu
authors: Hai-Yan
authors: HY
authors: Shi
authors: Yong-Zhen
authors: YZ
authors: Jiang
authors: Hong-Lan
authors: HL
authors: Zhao
authors: Feng
authors: F
authors: Wang
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D002681:China; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D016174:Hepacivirus / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D011933:Reagent Kits, Diagnostic
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic