title: Regulation on mechanical properties of collagen: enhanced bioactivities of metallofullerol.
abstract: Increased mechanical property of extracellular matrix (ECM) around tumor tissue is highly correlated to the progression of cancer, and now its efficient regulation is still a challenge. Here, we report that Gd@C82(OH)22-collagen composites greatly suppress the malignant progression of cancer cells in vitro, and the metallofullerol can efficiently reduce the mechanical property of collagen matrix. Further study indicates that Gd@C82(OH)22 can firmly bind to tropocollagen, facilitate the nuclei and microfibril formation. The interference to interactions among tropocollagens leads to decreased amount and disturbed structure of collagen fibers. C60(OH)24, the fullerol counterpart of Gd@C82(OH)22, is studied in parallel and their impacts on collagen are strikingly modest. The comparison data reveals that the enhanced bioactivity of Gd@C82(OH)22 is highly related with its surface-structure. This study is the first attempt to apply nanomedicines to manipulate the biophysical property of collagen matrix, providing a new sight to target ECM in cancer therapy.
  
  
  BACKGROUND
  Increased presence of "harder" collagen in the extracellular matrix (ECM) around the tumor tissue highly correlates with cancer progression. In this paper, a metallofullerol-based approach is reported as an efficient nanotechnology approach in reducing the mechanical properties of the synthesized collagen, paving the way to the development of novel anti-cancer therapies.
authors: CAS Key Laboratory for Biomedical Effects of Nanomaterial & Nanosafety, Institute of High Energy Physics, Chinese Academy of Science (CAS), Beijing 100049, China.
authors: Yan
authors: Y
authors: Song
authors: CAS Key Laboratory for Biomedical Effects of Nanomaterial & Nanosafety, Institute of High Energy Physics, Chinese Academy of Science (CAS), Beijing 100049, China.
authors: Mingyi
authors: M
authors: Zhang
authors: CAS Key Laboratory for Biomedical Effects of Nanomaterial & Nanosafety, Institute of High Energy Physics, Chinese Academy of Science (CAS), Beijing 100049, China.
authors: Lina
authors: L
authors: Zhao
authors: Department of Physics, Beijing Institute of Technology, Beijing 100081, China.
authors: Xiaohui
authors: X
authors: Yin
authors: CAS Key Laboratory for Biomedical Effects of Nanomaterial & Nanosafety, Institute of High Energy Physics, Chinese Academy of Science (CAS), Beijing 100049, China.
authors: Jiaohui
authors: J
authors: Zhao
authors: CAS Key Laboratory for Biomedical Effects of Nanomaterial & Nanosafety, Institute of High Energy Physics, Chinese Academy of Science (CAS), Beijing 100049, China.
authors: Juan
authors: J
authors: Li
authors: CAS Key Laboratory for Biomedical Effects of Nanomaterial & Nanosafety, Institute of High Energy Physics, Chinese Academy of Science (CAS), Beijing 100049, China.
authors: Rui
authors: R
authors: He
authors: CAS Key Laboratory for Biomedical Effects of Nanomaterial & Nanosafety, Institute of High Energy Physics, Chinese Academy of Science (CAS), Beijing 100049, China.
authors: Yanan
authors: Y
authors: Chang
authors: CAS Key Laboratory for Biomedical Effects of Nanomaterial & Nanosafety, Institute of High Energy Physics, Chinese Academy of Science (CAS), Beijing 100049, China.
authors: Junjiang
authors: J
authors: Jin
authors: CAS Key Laboratory for Biomedical Effects of Nanomaterial & Nanosafety, Institute of High Energy Physics, Chinese Academy of Science (CAS), Beijing 100049, China. Electronic address: zhao-office@ihep.ac.cn.
authors: Yuliang
authors: Y
authors: Zhao
authors: CAS Key Laboratory for Biomedical Effects of Nanomaterial & Nanosafety, Institute of High Energy Physics, Chinese Academy of Science (CAS), Beijing 100049, China. Electronic address: lijingyuan@ihep.ac.cn.
authors: Jingyuan
authors: J
authors: Li
authors: CAS Key Laboratory for Biomedical Effects of Nanomaterial & Nanosafety, Institute of High Energy Physics, Chinese Academy of Science (CAS), Beijing 100049, China. Electronic address: xinggm@ihep.ac.cn.
authors: Gengmei
authors: G
authors: Xing
mesh_terms: D000818:Animals; D024042:Collagen Type I / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry*; D037741:Fullerenes / Q000737:chemistry*; D005682:Gadolinium / Q000737:chemistry*
chemical_list: D024042:Collagen Type I; D037741:Fullerenes; D005682:Gadolinium
keywords: Collagen; Mechanical property; Metallofullerol; Structure-related bioactivity