title: Inhibition of proinflammatory cytokine by IL-25 in Vogt-Koyanagi-Harada syndrome.
abstract: OBJECTIVE
  Vogt-Koyanagi-Harada (VKH) syndrome is a multisystem disorder presumed to be mediated by an autoimmune response. Recent studies have shown that interleukin (IL) 25 was involved in the T-cell immune response. This study analyzed the expression and potential role of IL-25 in the pathogenesis of VKH syndrome.
  
  
  METHODS
  The IL-25 serum levels were determined by enzyme-linked immunosorbent assay (ELISA). The IL-1β, IL-6, and TNF-α level in supernatants of PBMCs cultured with LPS in the absence or presence of recombinant(r) IL-25 was detected by ELISA.
  
  
  RESULTS
  A significantly decreased serum IL-25 level was found in VKH patients. In vitro experiments showed that rIL-25 was able to significantly inhibit the production of IL-1β, IL-6, and TNF-α by PBMCs from active VKH patients.
  
  
  CONCLUSIONS
  IL-25 may be involved in the development of VKH syndrome, possibly by inhibiting the expression of proinflammatory cytokines.
authors: Department of Opthalmology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Lab of Ophthalmology, Chongqing Eye Institute , Chongqing , P. R. China .
authors: Mei
authors: M
authors: Xu
authors: Chaokui
authors: C
authors: Wang
authors: Yuan
authors: Y
authors: Tian
authors: Aize
authors: A
authors: Kijlstra
authors: Peizeng
authors: P
authors: Yang
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000473:pathology; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D020381:Interleukin-17 / Q000097:blood / Q000627:therapeutic use*; D008297:Male; D011994:Recombinant Proteins / Q000627:therapeutic use; D016896:Treatment Outcome; D014607:Uveomeningoencephalitic Syndrome / Q000097:blood / Q000188:drug therapy* / Q000276:immunology
chemical_list: D020381:Interleukin-17; D011994:Recombinant Proteins
keywords: Autoimmune disease; Vogt-Koyanagi-Harada syndrome; interleukin-25