title: Systemic evaluation of hypoxic-ischemic brain injury in neonatal rats.
abstract: The objective of the study was to evaluate the systematically rat model of neonatal hypoxic-ischemic brain damage. The right carotid arteries of 7-day-old healthy Wistar rats were ligated, and then, the rats were subjected to an environment with 8 % of oxygen. Four weeks after the birth, neurobehavioral test, water maze test, and motor-evoked potential and neuropathologic examinations were performed. The footprint analysis showed significantly larger and instable paces in the hypoxic-ischemic group (P < 0.05); the time that rats crossed the balance beam in the hypoxic-ischemic group was longer than the control group (P < 0.05). The water maze test showed that the escape latency of hypoxic-ischemic group was significantly longer than that of control group (P < 0.05). The hindlimb quadriceps compound muscle-evoked potential CMEP of rats in hypoxic-ischemic group showed that the wave amplitude was lower than that of control group (P < 0.05). HE staining showed visible periventricular leukomalacia in hypoxic-ischemic groups; disrupted nuclear membrane was detected in the IH group with transelectronmicroscopy; Immunohistochemistry: compared with control group, MBP-positive neurocytes decreased, glial fibrillary acidic protein positive neurocytes increased in the periventricular zone (P < 0.05). Carotid artery ligation combining the hypoxic chamber created a reliable and stable rat model of neonatal hypoxic-ischemic brain damage and can be used for experimental research related to management of cerebral palsy.
authors: Department of Neurosurgery, Wuxi Hospital of Integrated Traditional and Western Medicine, Wuxi, 214041, China.
authors: Ai-Hua
authors: AH
authors: Zhu
authors: Yan-Rong
authors: YR
authors: Hu
authors: Wei
authors: W
authors: Liu
authors: Feng
authors: F
authors: Gao
authors: Jian-Xin
authors: JX
authors: Li
authors: Li-Hui
authors: LH
authors: Zhao
authors: Gang
authors: G
authors: Chen
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D001921:Brain / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism / Q000473:pathology*; D008297:Male; D018782:Maze Learning; D051381:Rats; D017208:Rats, Wistar