title: Differences in neuronal damage and gliosis in the hippocampus between young and adult gerbils induced by long duration of transient cerebral ischemia.
abstract: Response to cerebral ischemia in young animals was very different from that in the adult. The aim of this study was to investigate differences in neuronal death and gliosis in the hippocampal CA1 region (CA1) between adult and young gerbils following 5 and 15 min of transient cerebral ischemia. Delayed neuronal death (DND) of pyramidal cells occurred in the CA1 was similar in all the adult gerbils after 5 and 15 min of ischemia: the DND occurred 4 days after ischemia. In the young groups, DND of pyramidal cells in the CA1 region occurred 7 and 3 days after 5 and 15 min of ischemia, respectively. On the other hand, the activation of GFAP-immunoreactive ((+)) astrocytes and Iba-1(+) microglia was different in the young groups from the adult groups after ischemia. The change pattern of GFAP immunoreactivity in the adult groups was similar in both the adult groups after ischemia; in the young groups, the activation of GFAP(+) astrocytes after 5 min of ischemia was much delayed than that after 15 min of ischemia. Activated Iba-1(+) microglia were aggregated in the stratum pyramidale 4 days after ischemia in all the adult ischemia-operated groups; in the young groups, activated Iba-1(+) microglia were aggregated in the stratum pyramidale 7 days after 5 min of ischemia and 3 days after 15 min of ischemia. These observations indicate that DND in young animals is very different from the adult according to different duration of transient cerebral ischemia and glial activation is very different in young animals after different duration of transient ischemia.
authors: Department of Integrative Traditional & Western Medicine, Medical College, Yangzhou University, Yangzhou, Jiangsu 225001, China.
authors: Bing Chun
authors: BC
authors: Yan
authors: Department of Emergency Medicine, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University, Seoul 150-950, South Korea.
authors: Taek Geun
authors: TG
authors: Ohk
authors: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
authors: Ji Hyeon
authors: JH
authors: Ahn
authors: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
authors: Joon Ha
authors: JH
authors: Park
authors: Department of Physiology, College of Medicine, Institute of Neurodegeneration and Neuroregeneration, Hallym University, Chuncheon 200-702, South Korea.
authors: Bai Hui
authors: BH
authors: Chen
authors: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
authors: Jae-Chul
authors: JC
authors: Lee
authors: Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan 330-714, South Korea.
authors: Choong Hyun
authors: CH
authors: Lee
authors: Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
authors: Myoung Cheol
authors: MC
authors: Shin
authors: Department of Anatomy and Cell Biology, College of Veterinary Medicine, Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.
authors: In Koo
authors: IK
authors: Hwang
authors: Department of Neurosurgery, Dongtan Sacred Heart Hospital, College of Medicine, Hallym University, Hwaseong 445-170, South Korea.
authors: Seung Myung
authors: SM
authors: Moon
authors: Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea; Institute of Medical Sciences, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea. Electronic address: cjhmd@kangwon.ac.kr.
authors: Jun Hwi
authors: JH
authors: Cho
authors: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea; Institute of Medical Sciences, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea. Electronic address: mhwon@hallym.ac.kr.
authors: Moo-Ho
authors: MH
authors: Won
mesh_terms: D000375:Aging; D000704:Analysis of Variance; D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism; D004195:Disease Models, Animal; D005452:Fluoresceins; D005849:Gerbillinae; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005911:Gliosis / Q000209:etiology*; D006624:Hippocampus / Q000473:pathology*; D002546:Ischemic Attack, Transient / Q000473:pathology*; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000473:pathology*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D013997:Time Factors
chemical_list: C100745:Aif1 protein, rat; D002135:Calcium-Binding Proteins; D005452:Fluoresceins; D005904:Glial Fibrillary Acidic Protein; D008840:Microfilament Proteins; C435731:fluoro jade; D010751:Phosphopyruvate Hydratase
keywords: Astrocytes; Delayed neuronal death; Ischemia–reperfusion injury; Microglia; Pyramidal neurons; Young gerbil