title: β-catenin mediates the inflammatory cytokine expression induced by the Der p 1 house dust mite allergen.
abstract: The modulations of β-catenin were analyzed during the inflammatory response induced by the Der p 1 house dust mite allergen. Der p 1 induced the dose-dependent expression of inflammatory cytokines, including interleukin (IL)-6, IL-8, monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-α (TNF-α) in THP-1 human monocytic cells. The mRNA expression levels of β-catenin were not altered, however protein levels increased following Der p 1 treatment, demonstrating that β-catenin was modulated by post-transcriptional processes. It was also revealed that nuclear β-catenin levels were significantly increased while cytoplasmic β-catenin levels were reduced, which demonstrated the nuclear translocation of β-catenin by the Der p 1 allergen. Glycogen synthase kinase 3β (GSK3β), a regulator of β-catenin stability, was demonstrated to be phosphorylated following Der p 1 treatment. When β-catenin was knocked down by the transfection of its small interfering RNA (siRNA), inflammatory cytokine expression as well as nuclear factor-κB (NF-κB) activity, which were induced by Der p 1 treatment, were all significantly reduced. The results demonstrated that Der p 1-induced inflammatory responses were mediated by β-catenin.
authors: Department of Microbiology, School of Medicine, Chung‑Ang University, Seoul 156-756, Republic of Korea.
authors: Jaewoong
authors: J
authors: Jang
authors: Department of Microbiology, School of Medicine, Chung‑Ang University, Seoul 156-756, Republic of Korea.
authors: Jong-Hyeok
authors: JH
authors: Ha
authors: School of Mechanical Engineering, Chung‑Ang University, Seoul 156-756, Republic of Korea.
authors: Seok-Min
authors: SM
authors: Kim
authors: Department of Microbiology, School of Medicine, Chung‑Ang University, Seoul 156-756, Republic of Korea.
authors: Wonyong
authors: W
authors: Kim
authors: Department of Microbiology, School of Medicine, Chung‑Ang University, Seoul 156-756, Republic of Korea.
authors: Kijeong
authors: K
authors: Kim
authors: Department of Microbiology, School of Medicine, Chung‑Ang University, Seoul 156-756, Republic of Korea.
authors: Sang-In
authors: SI
authors: Chung
authors: Department of Microbiology, School of Medicine, Chung‑Ang University, Seoul 156-756, Republic of Korea.
authors: Yoosik
authors: Y
authors: Yoon
mesh_terms: D000818:Animals; D039741:Antigens, Dermatophagoides / Q000008:administration & dosage / Q000276:immunology*; D060829:Arthropod Proteins / Q000008:administration & dosage*; D018932:Chemokine CCL2 / Q000276:immunology; D003546:Cysteine Endopeptidases / Q000008:administration & dosage*; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D015850:Interleukin-6 / Q000276:immunology; D016209:Interleukin-8 / Q000276:immunology; D039981:Pyroglyphidae / Q000276:immunology; D034741:RNA, Small Interfering; D015398:Signal Transduction / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D051176:beta Catenin / Q000276:immunology / Q000378:metabolism*
chemical_list: D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D015850:Interleukin-6; D016209:Interleukin-8; D034741:RNA, Small Interfering; D014409:Tumor Necrosis Factor-alpha; D051176:beta Catenin; D003546:Cysteine Endopeptidases; C451162:Dermatophagoides pteronyssinus antigen p 1