title: Uridinediphosphate-glucose: Isovitexin 7-O-glucosyltransferase from barley protoplasts: Subcellular localization.
abstract: Protoplasts isolated from 6-d-old primary leaves of barley (Hordeum vulgare L.) contain an enzyme which transfers the glucosyl moiety of uridine-diphosphateglucose to isovitexin, resulting in the formation of saponarin, the major flavonoid of barley. Purified chloroplasts isolated from protoplasts contained less than 2% of the total glucosyltransferase activity. These chloroplasts were 97% intact, based on ribulose-bisphosphate-carboxylase activity. Similarly low levels of glucosyltransferase activity were found in mitochondria and microbody or microsomal preparations from protoplasts. The soluble fraction (cytosol) contained at least 93% of the isovitexin 7-O-glucosyltransferase activity.
authors: Department of Botany, Miami University, 45056, Oxford, OH, USA.
authors: D E
authors: DE
authors: Blume
authors: J G
authors: JG
authors: Jaworski
authors: J W
authors: JW
authors: McClure