title: Ecotoxicological effects of a veterinary food additive, copper sulphate, on antioxidant enzymes and mRNA expression in earthworms.
abstract: The present study was aimed to investigate the effect of the veterinary food additive copper sulphate (CuSO₄) on the eco-toxicological responses of earthworms Eisenia fetida (E. fetida). The following biomarkers were measured: catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione-S-transferase (GST) activities. Gene expression analyses such as metallothionein (MT) and heat shock protein 70 (Hsp70) were also examined. A time-dependent increase of CAT activity was found at 400 mg/kg and SOD activity at 200 and 400 mg/kg. The highest expression of Hsp70 (4.4-fold) was observed at day 15 at 400 mg/kg. Our results indicated that the measured antioxidant enzymes (except GST) had the ability to provide antioxidant defenses against the stressor; and compared to expression of MT, expression of Hsp70 could be more reliable molecular tools with predictive possibilities to monitor the eco-toxicity of stressors such as feed additive CuSO₄.
authors: National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, South China Agricultural University, Guangzhou, People's Republic of China.
authors: Wenguang
authors: W
authors: Xiong
authors: National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, South China Agricultural University, Guangzhou, People's Republic of China.
authors: Xueyao
authors: X
authors: Ding
authors: National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, South China Agricultural University, Guangzhou, People's Republic of China.
authors: Yiming
authors: Y
authors: Zhang
authors: National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, South China Agricultural University, Guangzhou, People's Republic of China. Electronic address: sunyx@scau.edu.cn.
authors: Yongxue
authors: Y
authors: Sun
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D019327:Copper Sulfate / Q000494:pharmacology*; D005503:Food Additives / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D008668:Metallothionein / Q000235:genetics; D009835:Oligochaeta / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D016877:Oxidants / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D019155:Veterinary Drugs / Q000494:pharmacology
chemical_list: D005503:Food Additives; D018840:HSP70 Heat-Shock Proteins; D016877:Oxidants; D012333:RNA, Messenger; D019155:Veterinary Drugs; D008668:Metallothionein; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D019327:Copper Sulfate
keywords: Antioxidant; Contaminant; Copper sulphate; Eco-toxicity; Veterinary food additive