title: [Influence on cell proliferation by small interfering RNA of Cyclin Y expression in laryngeal cancer cells].
abstract: OBJECTIVE
  The effects of lentivirus-mediated suppression of Cyclin Y (CCNY) expression on the proliferation of laryngeal cancer cells were investigated in vitro.
  
  
  METHODS
  The lentivirus vectors containing a small hairpin RNA (shRNA) to target CCNY were constructed.Hep-2 cells were divided into the following two experimental groups:the negative control group (control lentivirus infected cells) and CCNY knockdown group (CCNY shRNA-expressing lentivirus infected cells). After Hep-2 cells were infected, Real-time PCR was used to measure CCNY expression. The influence of CCNY on the proliferation of laryngeal cancer cells were assessed using MTT and colony formation experiments.Each experiment was performed in triplicate and repeated three times.
  
  
  RESULTS
  Lentiviruses expressing shRNA against CCNY were constructed and Hep-2 cells were infected with above mentioned lentivirus at MOI (Multiplicity of infection) of 120.Real-time PCR analysis showed that the mRNA expression of CCNY in Hep-2 cells in the knockdown group was significantly decreased (P < 0.05); the mRNA level of CCNY was 75.3% lower in the si-CCNY group than in the si-CTRL group. After 5 days of lentiviral infection, the cell viability was significantly lower in cells infected with the CCNY-shRNA lentivirus compared to cells infected with the control lentivirus following a 6-day incubation. The colony number was decreased by 60% in Hep-2 cells infected with the CCNY-shRNA-lentivirus infected cells following a 10-day incubation.
  
  
  CONCLUSIONS
  The results suggested that lentivirus-mediated downregulation of CCNY expression decreased the proliferation and growth potency of laryngeal cancer cells.Lentiviruses delivering shRNA against CCNY may be a promising tool for laryngeal cancer therapy.
authors: Beijing Key Laboratory for Pediatric Otolaryngology, Head and Neck Science, Beijing Pediatric Research Institute,Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.
authors: Jun
authors: J
authors: Tai
authors: Ai-Dong
authors: AD
authors: Li
authors: Yuan-Sheng
authors: YS
authors: Rao
authors: Yu-Bei
authors: YB
authors: Huang
authors: Zhi-Gang
authors: ZG
authors: Huang
authors: Zhen-Kun
authors: ZK
authors: Yu
authors: Xiao-Hong
authors: XH
authors: Chen
authors: Wei-Guo
authors: WG
authors: Zhou
authors: Xiao
authors: X
authors: Xiao
authors: Shen
authors: S
authors: Wang
authors: Yang
authors: Y
authors: Han
authors: Qiao-Yin
authors: QY
authors: Liu
authors: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100029, China. Email:fangjugao@vip.sohu.com.
authors: Ju-Gao
authors: JG
authors: Fang
authors: Beijing Key Laboratory for Pediatric Otolaryngology, Head and Neck Science, Beijing Pediatric Research Institute,Beijing Children's Hospital, Capital Medical University, Beijing 100045, China. Email:nixin@bch.com.cn.
authors: Xin
authors: X
authors: Ni
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016213:Cyclins; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism; D016086:Lentivirus / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D016213:Cyclins; D034741:RNA, Small Interfering