title: "Brachyspira hampsonii" clade I isolated from Belgian pigs imported to Germany.
abstract: This report describes the detection of "Brachyspira (B.) hampsonii" clade I in Belgian pigs imported to Germany. Two of seventeen pigs from one herd were reported positive for Brachyspira hyodysenteriae by culture in a Belgian diagnostic laboratory, but negative for this Brachyspira species by specific PCR. In this study, from 22 fecal samples and 2 colon contents of these animals various Brachyspira species were cultured and identified by nox-RFLP as Brachyspira murdochii, Brachyspira innocens and Brachyspira intermedia. Albeit the six B. intermedia isolates proved to be negative in a species specific PCR. Sequencing of the nox-gene of three of these isolates revealed that the sequences were 99% identical to published sequences of "B. hampsonii" clade I. From one pig which was positive for "B. hampsonii" clade I histopathology was done and showed moderate lesions consistent with brachyspiral disease.
authors: Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine, Hannover, Germany. Electronic address: Jurohde@gmx.de.
authors: Judith
authors: J
authors: Rohde
authors: Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine, Hannover, Germany.
authors: Kerstin
authors: K
authors: Habighorst-Blome
authors: Department of Pathology, University of Veterinary Medicine, Hannover, Germany.
authors: Frauke
authors: F
authors: Seehusen
mesh_terms: D000818:Animals; D001530:Belgium; D017400:Brachyspira / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004403:Dysentery / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D005243:Feces / Q000382:microbiology; D005858:Germany; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D034421:Sus scrofa / Q000382:microbiology; D013552:Swine / Q000382:microbiology; D013553:Swine Diseases / Q000175:diagnosis* / Q000382:microbiology* / Q000473:pathology
keywords: nox; swine dysentery; “Brachyspira hampsonii”