title: Prevalence of FA-D2 rare complementation group of Fanconi anemia in Serbia.
abstract: OBJECTIVE
  To investigate genetic subtypes of inherited bone marrow failure syndrome Fanconi anemia (FA) in Sebia. FA-D2 subtype was found to be the most frequent genetic subtype among investigated FA patients; specific observations of FA-D2 phenotype are pointed out.
  
  
  METHODS
  Several biological endpoints of FA cells in vitro such as radiation-induced level of lymphocyte micronuclei (radiosensitivity), base line and radiation induced level of the DNA double strand breaks (DSBs), leukocyte apoptosis, and telomere capping function were assessed.
  
  
  RESULTS
  The results indicate that all FA-D2 patients display radioresistant in vitro response, which is seen as significantly reduced yield of radiation-induced micronuclei. On the contrary, FA-A patients display radiosensitive in vitro response seen as increased number of radiation-induced micronuclei (MN). A massive elimination of irradiated cells via apoptosis is found in both FA-A and FA-D2 subtypes. In FA-A subtype apoptosis positively relates with the yield of radiation-induced MN, whereas in FA-D2 subtype apoptosis relates with a high percentage of cells carrying dysfunctional telomeres. The present results unequivocally demonstrate that cytokinesis-block micronucleus (CBMN) assay and analyses of telomere capping function can be used to distinguish FA-D2 and FA-A complementation groups.
  
  
  CONCLUSIONS
  Considering all biological endpoints were analyzed, it can be concluded that all FA patients are radiosensitive, regardless of their complementation group. Thus, using CBMN test and telomere capping function analysis can discriminate FA-A from FA-D2 complementation groups, which could be important for assessment the conditioning regimens prior to bone marrow transplantation.
authors: Medical School, University of Belgrade, Belgrade, Serbia.
authors: Vujić
authors: V
authors: Dragana
authors: Petrović
authors: P
authors: Sandra
authors: Lazić
authors: L
authors: Emilija
authors: Kuzmanović
authors: K
authors: Miloš
authors: Leskovac
authors: L
authors: Andreja
authors: Joksić
authors: J
authors: Ivana
authors: Mićić
authors: M
authors: Dragan
authors: Jovanović
authors: J
authors: Ankica
authors: Zečević
authors: Z
authors: Zeljko
authors: Guć-Šćekić
authors: GŠ
authors: Marija
authors: Cirković
authors: C
authors: Sanja
authors: Joksić
authors: J
authors: Gordana
mesh_terms: D017209:Apoptosis; D002648:Child; D002675:Child, Preschool; D053903:DNA Breaks, Double-Stranded; D005199:Fanconi Anemia / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D010641:Phenotype; D015995:Prevalence; D055771:Serbia / Q000453:epidemiology