title: Antitags: SERS-encoded nanoparticle assemblies that enable single-spot multiplex protein detection.
abstract: Simultaneous detection of multiple proteins on a single spot can be efficiently achieved by using multiplexed surface-enhanced Raman spectroscopy (SERS)-encoded nanoparticle 'antitags' consisting of poly(ethylene glycol) (PEG)-protected silver dimers (and higher aggregates) and antibody-tagging entities. The effective SERS-based multivariate deconvolution approach guarantees an accurate and successful distinguishable identification of single and multiple proteins in complex samples. Their potential application in multiplexed SERS bioimaging technology can be easily envisaged.
authors: Instituto de Ciencia y Tecnología de Polímeros (ICTP), Consejo Superior de Investigaciones Científicas (CSIC), Juan de la Cierva 3, Madrid, 28006, Spain.
authors: Nekane
authors: N
authors: Guarrotxena
authors: Guillermo C
authors: GC
authors: Bazan
mesh_terms: D000906:Antibodies / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D002097:C-Reactive Protein / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D055503:Protein Multimerization; D011506:Proteins / Q000737:chemistry*; D018030:Silver Compounds / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013917:Thrombin / Q000737:chemistry
chemical_list: C516314:1,4-benzenedithiol; D000906:Antibodies; D001713:Biphenyl Compounds; D011506:Proteins; D018030:Silver Compounds; D013438:Sulfhydryl Compounds; D011092:Polyethylene Glycols; D002097:C-Reactive Protein; D013917:Thrombin
keywords: biosensors; multiplex; nanoparticles; silver; surface enhanced raman spectroscopy (SERS)