title: Isolation and functional characterization of the ShCBF1 gene encoding a CRT/DRE-binding factor from the wild tomato species Solanum habrochaites.
abstract: Plant growth and productivity are greatly affected by low ambient temperature. Complex cascades of gene expression in cold stress response are regulated by transcription factors. In this study, a cDNA clone, named ShCBF1, was isolated from Solanum habrochaites seedlings (a wild relative of cultivated tomato). It was classified as one of CBF family members based on multiple sequence alignment. The expression analysis confirmed that ShCBF1 was induced by low temperature, high salinity and drought stress. Experiments of subcellular localization in tobacco leaf cells indicated that it was localized in nucleus. Transient expression assay using onion epidermal cells revealed that the ShCBF1 protein could function similarly to AtCBF1 in activating the expression of reporter genes with a CRT/DRE element in their promoter. Moreover, ectopic overexpression of ShCBF1 in Arabidopsis enhanced freezing and high salinity tolerance of transgenic plants by improving the expression levels of some stress-responsive marker genes. Taken together, our results suggest that ShCBF1 behaves as a typical plant CBF transcription factor and might be involved in plant response to various environmental stresses.
authors: College of Horticulture, Northeast Agricultural University, Harbin 150030, PR China; College of Life Sciences, Northeast Agricultural University, Harbin 150030, PR China.
authors: Zhenjun
authors: Z
authors: Li
authors: College of Life Sciences, Northeast Agricultural University, Harbin 150030, PR China; Heilongjiang Provincial Key University Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, PR China.
authors: Lili
authors: L
authors: Zhang
authors: College of Horticulture, Northeast Agricultural University, Harbin 150030, PR China.
authors: Jingfu
authors: J
authors: Li
authors: College of Horticulture, Northeast Agricultural University, Harbin 150030, PR China.
authors: Xiangyang
authors: X
authors: Xu
authors: Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, PR China.
authors: Quanhong
authors: Q
authors: Yao
authors: College of Horticulture, Northeast Agricultural University, Harbin 150030, PR China; College of Life Sciences, Northeast Agricultural University, Harbin 150030, PR China; Heilongjiang Provincial Key University Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, PR China. Electronic address: axwang@neau.edu.cn.
authors: Aoxue
authors: A
authors: Wang
mesh_terms: D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D032322:Solanum / Q000235:genetics*; D013347:Subcellular Fractions / Q000378:metabolism; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D015534:Trans-Activators
keywords: CRT/DRE; High salinity; Low temperature; ShCBF1; Solanum habrochaites