title: Efficient conversion of myricetin from Ampelopsis grossedentata extracts and its purification by MIP-SPE.
abstract: In this study, we developed an efficient conversion process of dihydromyricetin to myricetin from Ampelopsis grossedentata extracts. The content of myricetin increased from 2.38% to 85.57%, demonstrating the successful dehydrogenation of dihydromyricetin. Molecularly imprinted polymers (MIPs) were prepared by surface imprinting method using silica microspheres as the support matrices and myricetin as template. The MIPs were applied for the selective adsorption of myricetin. The chemical structure of the MIPs was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy. Static, dynamic and selective adsorption experiments showed that the MIPs exhibited good adsorption ability, rather fast template rebinding kinetics, and appreciate selectivity over structurally related compounds. Accordingly, the MIPs were applied as the selective sorbent in SPE to purify myricetin obtained through dehydrogenation, followed by HPLC-UV analysis. The recoveries of myricetin and dihydromyricetin were 92.7% and 55.6%, respectively. This study demonstrates the feasibility of using the developed MIP-SPE method to purify and enrich myricetin in the natural products.
authors: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors: Shian
authors: S
authors: Zhong
authors: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors: Yanyue
authors: Y
authors: Kong
authors: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors: Ling
authors: L
authors: Zhou
authors: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors: Chengyun
authors: C
authors: Zhou
authors: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors: Xiaona
authors: X
authors: Zhang
authors: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. Electronic address: yanwangcsu@163.com.
authors: Yan
authors: Y
authors: Wang
mesh_terms: D032421:Ampelopsis / Q000737:chemistry*; D000975:Antioxidants / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005419:Flavonoids / Q000302:isolation & purification*; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D011108:Polymers; C040015:myricetin
keywords: Ampelopsis grossedentata; Dehydrogenation; Molecularly imprinted microspheres; Myricetin; SPE