title: Quantitative and pattern recognition analyses of magnoflorine, spinosin, 6'''-feruloyl spinosin and jujuboside A by HPLC in Zizyphi Semen.
abstract: Two rapid and simple HPLC methods with UV detector to determine three main compounds (magnoflorine, spinosin and 6'''-feruloyl spinosin) and evaporative light scattering detector (ELSD) to determine jujuboside A were developed for the chemical analyses of Zizyphi Semen. Magnoflorine, spinosin, and 6'''-feruloyl spinosin were separated with an YMC J'sphere ODS-H80 column (250 mm × 4.6 mm, 4 μm) by the gradient elution followed by the isocratic elution using methanol with 0.1 % formic acid and water with 0.1 % formic acid as the mobile phase. The flow rate was 1.0 mL/min. Jujuboside A was separated by HPLC-ELSD with YoungJinBioChrom Aegispak C18-L column (250 mm × 4.6 mm, 5 μm) column in a gradient elution using methanol with 0.1 % formic acid (A) and water with 0.1 % formic acid as the mobile phase. These two methods were fully validated with respect to linearity, precision, accuracy, stability, and robustness. These HPLC methods were applied successfully to quantify four compounds in a Zizyphi Semen extract. The HPLC analytical methods were validated for pattern recognition analysis by repeated analysis of 91 seed samples corresponding to 48 Zizyphus jujuba var. spinosa (J01-J48) and 43 Zizyphus mauritiana (M01-M43). The results indicate that these methods are suitable for a quality evaluation of Zizyphi Semen.
authors: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 712-702, Korea.
authors: Won Il
authors: WI
authors: Kim
authors: Bing Tian
authors: BT
authors: Zhao
authors: Hai Yan
authors: HY
authors: Zhang
authors: Je Hyun
authors: JH
authors: Lee
authors: Jong Keun
authors: JK
authors: Son
authors: Mi Hee
authors: MH
authors: Woo
mesh_terms: D000700:Analgesics / Q000737:chemistry; D000927:Anticonvulsants / Q000737:chemistry*; D001060:Aporphines / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000032:analysis / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D020834:Ethnopharmacology; D047309:Flavones / Q000032:analysis / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D055670:Medicine, Korean Traditional; D009391:Nephelometry and Turbidimetry; D010363:Pattern Recognition, Automated; D010936:Plant Extracts / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis* / Q000737:chemistry; D012639:Seeds / Q000737:chemistry*; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet; D014149:Tranquilizing Agents / Q000737:chemistry; D031957:Ziziphus / Q000737:chemistry*
chemical_list: D000700:Analgesics; D000927:Anticonvulsants; D001060:Aporphines; D004187:Disaccharides; D004365:Drugs, Chinese Herbal; D047309:Flavones; D005419:Flavonoids; D010936:Plant Extracts; D012503:Saponins; D014149:Tranquilizing Agents; C087808:jujuboside A; C055669:spinosin; C001670:magnoflorine