title: Proteins related to the spindle and checkpoint mitotic emphasize the different pathogenesis of hypoplastic MDS.
abstract: Some studies show that alterations in expression of proteins related to mitotic spindle (AURORAS KINASE A and B) and mitotic checkpoint (CDC20 and MAD2L1) are involved in chromosomal instability and tumor progression in various solid and hematologic malignancies. This study aimed to evaluate these genes in MDS patients. The cytogenetics analysis was carried out by G-banding, AURKA and AURKB amplification was performed using FISH, and AURKA, AURKB, CDC20 and MAD2L1 gene expression was performed by qRT-PCR in 61 samples of bone marrow from MDS patients. AURKA gene amplification was observed in 10% of the cases, which also showed higher expression levels than the control group (p=0.038). Patients with normo/hypercellular BM presented significantly higher expression levels than hypocellular BM patients, but normo and hypercellular BM groups did not differ. After logistic regression analysis, our results showed that HIGH expression levels were associated with increased risk of developing normo/hypercellular MDS. It also indicated that age is associated with AURKA, CDC20 and MAD2L1 HIGH expression levels. The distinct expression of hypocellular patients emphasizes the prognostic importance of cellularity to MDS. The amplification/high expression of AURKA suggests that the increased expression of this gene may be related to the pathogenesis of disease.
authors: Cancer Institute of Ceará/A.C. Camargo Hospital (Dinter-Minter), Fortaleza, Ceará, Brazil.
authors: Fabiola Fernandes
authors: FF
authors: Heredia
authors: Federal University of Ceará, Brazil.
authors: Juliana Cordeiro
authors: JC
authors: de Sousa
authors: Federal University of Ceará, Brazil.
authors: Howard Lopes
authors: HL
authors: Ribeiro Junior
authors: A.C. Camargo Hospital, São Paulo, Brazil.
authors: Alex Fiorini
authors: AF
authors: Carvalho
authors: Department of Clinical Medicine, Division of Hematology, Walter Cantidio Hospital, Federal University of Ceará, Brazil.
authors: Silvia Maria Meira
authors: SM
authors: Magalhaes
authors: Department of Clinical Medicine, Division of Hematology, Walter Cantidio Hospital, Federal University of Ceará, Brazil. Electronic address: ronaldpinheiro@pq.cnpq.br.
authors: Ronald Feitosa
authors: RF
authors: Pinheiro
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D059447:Cell Cycle Checkpoints / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000502:physiology*; D005260:Female; D006801:Humans; D059785:Karyotype; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000145:classification / Q000235:genetics / Q000473:pathology*; D008941:Spindle Apparatus / Q000502:physiology*; D055815:Young Adult
chemical_list: D018797:Cell Cycle Proteins
keywords: AURKA; AURKB; CDC20; MAD2; Myelodysplastic syndromes