title: Antioxidant and immunological activity in vitro of polysaccharides from Phellinus nigricans mycelia.
abstract: Three polysaccharide fractions, namely, PNMP 1, PNMP2, and PNMP3, were isolated from Phellinus nigricans mycelia. The average molecular weights of PNMP1, PNMP2, and PNMP3 were approximately 28.4, 31.5, and 26.1 kDa, respectively. PNMP2 and PNMP3 comprised six monosaccharides: arabinose, fucose, glucose, galactose, mannose, and xylose. PNMP1 comprised only four monosaccharides: glucose, galactose, mannose, and xylose. In vitro antioxidant tests showed that PNMP2 and PNMP3 significantly scavenged superoxide radical, hydroxyl radical, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. PNMP1 effectively inhibited DPPH radical. Lymphocyte proliferation was significantly increased by PNMP1, PNMP2, and PNMP3, particularly by PNMP2 and PNMP3 in the presence of concanavalin A or lipopolysaccharide as mitogens for lymphocytes.
authors: College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, PR China. Electronic address: wangzy125@gmail.com.
authors: Zhanyong
authors: Z
authors: Wang
authors: College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, PR China.
authors: Feng
authors: F
authors: Zhou
authors: College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, PR China.
authors: Yue
authors: Y
authors: Quan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000494:pharmacology; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D007155:Immunologic Factors / Q000737:chemistry / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D025282:Mycelium / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D062610:Fungal Polysaccharides; D007155:Immunologic Factors
keywords: Antioxidant; Lymphocyte proliferation; Phellinus nigricans