title: Protease-deficient Saccharomyces cerevisiae strains for the synthesis of human-compatible glycoproteins.
abstract: Saccharomyces cerevisiae strains engineered previously to produce proteins with mammalian high mannose structures showed severe growth defects and decreased protein productivity. In strain YAB101, derived from one of these strains by a mutagenesis technique based on the disparity theory of evolution, these undesirable phenotypes were alleviated. Here we describe further engineering of YAB101 with the aim of synthesizing heterologous glycoproteins with Man5GlcNAc2, an intermediate for the mammalian hybrid and complex type oligosaccharides. About 60% conversion of Man8GlcNAc2 to Man5GlcNAc2 was observed after integration of Aspergillus saitoi α-1,2-mannosidase fused to the transmembrane domain of S. cerevisiae Och1. To obtain a higher yield of the target protein, a protease-deficient version of this strain was generated by disruption of PEP4 and PRB1, resulting in YAB101-4. Inactivation of these vacuolar proteases enhanced the secretion of human interferon-β by approximately 10-fold.
authors: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST).
authors: Kazuya
authors: K
authors: Tomimoto
authors: Yasuko
authors: Y
authors: Fujita
authors: Tomoko
authors: T
authors: Iwaki
authors: Yasunori
authors: Y
authors: Chiba
authors: Yoshifumi
authors: Y
authors: Jigami
authors: Ken-ichi
authors: K
authors: Nakayama
authors: Yoshihiro
authors: Y
authors: Nakajima
authors: Hiroko
authors: H
authors: Abe
mesh_terms: D001230:Aspergillus / Q000235:genetics; D005818:Genetic Engineering; D006023:Glycoproteins / Q000096:biosynthesis*; D006031:Glycosylation; D006801:Humans; D016899:Interferon-beta / Q000096:biosynthesis; D009844:Oligosaccharides / Q000096:biosynthesis / Q000737:chemistry; D010447:Peptide Hydrolases / Q000172:deficiency*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D014617:Vacuoles / Q000201:enzymology
chemical_list: D006023:Glycoproteins; D009844:Oligosaccharides; D016899:Interferon-beta; D010447:Peptide Hydrolases