title: Axonal degeneration in dorsal columns of spinal cord does not induce recruitment of hematogenous macrophages.
abstract: It is generally accepted that there are two populations of macrophages that respond to neural injuries and successful recruitment of hematogenous macrophages has been shown to help the process of nerve repair in the peripheral nervous system (PNS). Meanwhile, the recruitment of circulating macrophages after central nerve system (CNS) injuries is considered mild and delayed. We compared the recruitment of circulating macrophages in the peripheral nerves and spinal cord after dorsal root ganglionectomies, which induce selective and approximately similar extent of sensory fiber degeneration in PNS and CNS, in bone marrow chimeric mice. Our results showed that circulating macrophages were efficiently recruited in PNS but virtually no recruitment in CNS despite degeneration of peripheral and central sensory projections emanating from the same dorsal root ganglion (DRG) neurons. The mechanisms that prevent recruitment of circulating macrophages in CNS after injury remain poorly elucidated.
authors: Department of Neurology, University of Texas Medical School at Houston, 6431 Fannin Street, Houston, TX 77030, USA. Electronic address: gang.zhang@uth.tmc.edu.
authors: Gang
authors: G
authors: Zhang
authors: Department of Neurology, Johns Hopkins University, 600 North Wolfe Street, Baltimore, MD 21287, USA. Electronic address: phoffman@jhmi.edu.
authors: Paul N
authors: PN
authors: Hoffman
authors: Department of Neurology, University of Texas Medical School at Houston, 6431 Fannin Street, Houston, TX 77030, USA. Electronic address: kazim.sheikh@uth.tmc.edu.
authors: Kazim A
authors: KA
authors: Sheikh
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D016026:Bone Marrow Transplantation / Q000379:methods; D002490:Central Nervous System / Q000473:pathology / Q000503:physiopathology; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005434:Flow Cytometry; D015171:Ganglionectomy / Q000379:methods; D049452:Green Fluorescent Proteins / Q000235:genetics; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009410:Nerve Degeneration / Q000209:etiology* / Q000473:pathology*; D059348:Peripheral Nerve Injuries / Q000150:complications* / Q000601:surgery; D012584:Sciatic Nerve / Q000473:pathology; D013126:Spinal Nerve Roots / Q000473:pathology*; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D016207:Cytokines; D049452:Green Fluorescent Proteins; C067980:CD68 antigen, human; D000097382:CD68 Molecule
keywords: Axonal degeneration; Bone marrow chimeric mice; CNS; Chemokine; Cytokines; DRG; Dorsal root ganglionectomy; FACS; GFP; Hematogenous macrophage recruitment; PBMCs; PNS; central nerve system; dorsal root ganglion; fluorescence-activated cell sorting; green fluorescent protein; peripheral blood mononuclear cells; peripheral nerve system