title: Inositol based non-viral vectors for transgene expression in human cervical carcinoma and hepatoma cell lines.
abstract: Myo-Inositol (INO) is a biomolecule with crucial functions in many aspects. In this study, hyperbranched copolymers for gene delivery were synthesized based on inositol and low molecular weight polyethylenimine. The capacity of INO-PEIs to load plasmid DNA and their biocompatibility was demonstrated. A tumor target ligand, folic acid (FA), which was widely used for drug delivery systems, was subsequently conjugated to INO-PEIs and resulted in INO-PEI-FA copolymers. The polymers were then evaluated on their activity to mediate transgene expression in mammalian cell lines. As indicated, INO-PEIs were able to mediate efficient transgene expression, which was particularly noticeable in carcinoma cell line HeLa. INO-PEI-FA further improved the efficiency in HepG2. Distribution of INO-PEI-FA polymers in non-carcinoma NIH 3T3 and carcinoma HeLa cell lines was discussed.
authors: State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, PR China. Electronic address: zhangl@fjirsm.ac.cn.
authors: Lei
authors: L
authors: Zhang
authors: Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
authors: Yiwen
authors: Y
authors: Fan
authors: State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, PR China. Electronic address: wuyk@fjirsm.ac.cn.
authors: Yunkun
authors: Y
authors: Wu
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D005492:Folic Acid / Q000737:chemistry; D005822:Genetic Vectors / Q000737:chemistry* / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D007294:Inositol / Q000737:chemistry*; D008113:Liver Neoplasms / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D046529:Microscopy, Electron, Transmission; D041681:NIH 3T3 Cells; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D007294:Inositol; D005492:Folic Acid
keywords: Folic acid (FA); Hyperbranched molecules; Inositol (INO); Polyethylenimine (PEI); Transfection; Transgene expression