title: Bioactive self-assembled peptide nanofibers for corneal stroma regeneration.
abstract: Defects in the corneal stroma caused by trauma or diseases such as macular corneal dystrophy and keratoconus can be detrimental for vision. Development of therapeutic methods to enhance corneal regeneration is essential for treatment of these defects. This paper describes a bioactive peptide nanofiber scaffold system for corneal tissue regeneration. These nanofibers are formed by self-assembling peptide amphiphile molecules containing laminin and fibronectin inspired sequences. Human corneal keratocyte cells cultured on laminin-mimetic peptide nanofibers retained their characteristic morphology, and their proliferation was enhanced compared with cells cultured on fibronectin-mimetic nanofibers. When these nanofibers were used for damaged rabbit corneas, laminin-mimetic peptide nanofibers increased keratocyte migration and supported stroma regeneration. These results suggest that laminin-mimetic peptide nanofibers provide a promising injectable, synthetic scaffold system for cornea stroma regeneration.
authors: Institute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent University, Ankara 06800, Turkey.
authors: G
authors: G
authors: Uzunalli
authors: Institute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent University, Ankara 06800, Turkey.
authors: Z
authors: Z
authors: Soran
authors: Institute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent University, Ankara 06800, Turkey.
authors: T S
authors: TS
authors: Erkal
authors: Institute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent University, Ankara 06800, Turkey.
authors: Y S
authors: YS
authors: Dagdas
authors: Department of Ophthalmology, School of Medicine, Mersin University, Mersin, Turkey.
authors: E
authors: E
authors: Dinc
authors: Department of Ophthalmology, Gazi University, Ankara, Turkey.
authors: A M
authors: AM
authors: Hondur
authors: Department of Ophthalmology, Gazi University, Ankara, Turkey.
authors: K
authors: K
authors: Bilgihan
authors: Department of Ophthalmology, Gazi University, Ankara, Turkey. Electronic address: baydunus@yahoo.com.
authors: B
authors: B
authors: Aydin
authors: Institute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent University, Ankara 06800, Turkey. Electronic address: moguler@unam.bilkent.edu.tr.
authors: M O
authors: MO
authors: Guler
authors: Institute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent University, Ankara 06800, Turkey. Electronic address: atekinay@bilkent.edu.tr.
authors: A B
authors: AB
authors: Tekinay
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D003319:Corneal Stroma / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D011817:Rabbits; D012038:Regeneration / Q000187:drug effects*; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D010455:Peptides; D013501:Surface-Active Agents
keywords: Corneal stroma regeneration; Peptide amphiphile; RGD; Self-assembly; YIGSR