title: Absence of a role for interleukin-13 in inflammatory bowel disease.
abstract: IL-13 has been implicated in the pathogenesis of ulcerative colitis (UC), and may have a role in animal models of gut fibrosis. We studied the involvement of IL-13 in inflammation and fibrosis in UC and Crohn's disease (CD). Intestinal biopsies and anti-CD3/CD28- or anti-CD2/CD28-stimulated lamina propria mononuclear cells from UC and CD patients and control subjects were cultured, and IL-13, IL-4, IL-5, IL-17A and IFN-γ production was measured. Mucosal IL-13-producing cells were characterised by flow cytometry. Gut explants from strictured CD, non-strictured CD and healthy donors were cultured ex vivo, and secreted IL-13, IL-1β and collagen were measured. IL-13 production by mucosal explants and activated lamina propria mononuclear cells did not differ between CD, UC and control subjects, and was at least a log lower than IFN-γ and IL-17A. IL-13-producing cells, and in particular natural killer T cells, were uniformly low in all groups. IL-4 and IL-5 were undetectable in culture supernatants. Explants of CD strictures produced low amounts of IL-13, whereas IL-1β and collagen were elevated. We could not confirm that UC or strictured CD are associated with elevated IL-13 production. These data suggest that an anti-IL-13 Ab would not be an appropriate therapeutic strategy in inflammatory bowel disease.
authors: Centre for Immunology and Infectious Disease, Blizard Institute, Barts and the London School of Medicine and Dentistry, London, UK; Department of Internal Medicine, S. Matteo Hospital, Centro per lo Studio e la Cura delle Malattie Infiammatorie Croniche Intestinali, University of Pavia, Pavia, Italy.
authors: Paolo
authors: P
authors: Biancheri
authors: Antonio
authors: A
authors: Di Sabatino
authors: Francesca
authors: F
authors: Ammoscato
authors: Federica
authors: F
authors: Facciotti
authors: Flavio
authors: F
authors: Caprioli
authors: Renata
authors: R
authors: Curciarello
authors: Syed S
authors: SS
authors: Hoque
authors: Amir
authors: A
authors: Ghanbari
authors: Ijeoma
authors: I
authors: Joe-Njoku
authors: Paolo
authors: P
authors: Giuffrida
authors: Laura
authors: L
authors: Rovedatti
authors: Jens
authors: J
authors: Geginat
authors: Gino R
authors: GR
authors: Corazza
authors: Thomas T
authors: TT
authors: MacDonald
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018106:CD28 Antigens / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D003093:Colitis, Ulcerative / Q000276:immunology* / Q000473:pathology; D003424:Crohn Disease / Q000276:immunology* / Q000473:pathology; D005355:Fibrosis / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007249:Inflammation / Q000276:immunology* / Q000473:pathology; D015212:Inflammatory Bowel Diseases / Q000276:immunology* / Q000473:pathology; D007371:Interferon-gamma / Q000276:immunology; D018793:Interleukin-13 / Q000276:immunology*; D053661:Interleukin-13 Receptor alpha1 Subunit / Q000276:immunology; D053663:Interleukin-13 Receptor alpha2 Subunit / Q000276:immunology; D007413:Intestinal Mucosa / Q000276:immunology / Q000473:pathology; D007422:Intestines / Q000276:immunology / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000473:pathology; D008264:Macrophages / Q000276:immunology / Q000473:pathology; D008875:Middle Aged; D009092:Mucous Membrane / Q000276:immunology / Q000473:pathology; D055611:Natural Killer T-Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D053773:Transforming Growth Factor beta1 / Q000276:immunology; D055815:Young Adult
chemical_list: D018106:CD28 Antigens; D017252:CD3 Complex; C578095:IL13 protein, human; D018793:Interleukin-13; D053661:Interleukin-13 Receptor alpha1 Subunit; D053663:Interleukin-13 Receptor alpha2 Subunit; D053773:Transforming Growth Factor beta1; D007371:Interferon-gamma
keywords: Crohn's disease; Fibrosis; T helper cell type 2; Ulcerative colitis