title: Detection and genotype analysis of human papillomavirus in non-small cell lung cancer patients.
abstract: Although the role of human papillomavirus (HPV) in the development of uterine cervical cancer is well established, the role of HPV in lung carcinogenesis remains controversial. The detection rates of HPV DNA are subject to a wide variation from 0 to 100%. This is partly influenced by the detection techniques employed. To elucidate the impact of HPV infection on lung parenchyma, we analyzed 100 non-small cell lung cancer (NSCLC) specimens (39 squamous cell carcinomas, 50 adenocarcinomas, 5 samples with characteristics of both squamous cell and adenocarcinoma, 5 undifferentiated and 1 large cell carcinoma) from the region of Crete, Greece. Sixteen non-cancerous samples served as the negative controls. DNA was extracted from 100 paraffin-embedded tissue sections obtained from NSCLC patients. The specimens were examined for the detection of HPV DNA by Real-Time PCR using GP5+/GP6+ primers. Furthermore, the HPV-positive samples were subjected to genotyping. In contrast to the absence of viral genomes in the control samples, HPV DNA was detected in 19 NSCLC specimens (19%). In particular, 4 squamous cell carcinomas, 12 adenocarcinomas, 1 sample with characteristics of both squamous cell and adenocarcinoma, and 2 undifferentiated samples were HPV-positive. The distribution of HPV genotypes was as follows: HPV 16: eight cases (42.1%); HPV 11: three cases (15.8%); HPV 6: one case (5.2%); HPV 59: one case (5.2%); HPV 33: two cases (10.5%); HPV 31: two cases (10.5%) and HPV 18: two cases (10.5%). The presence of HPV in the tumor samples provides evidence of the potential role of HPV in NSCLC and strongly argues for additional research on this issue.
authors: Laboratory of Clinical Virology, Medical School, University of Crete, Heraklion, 71003, Crete, Greece.
authors: Emmanouela
authors: E
authors: Sarchianaki
authors: Stavros P
authors: SP
authors: Derdas
authors: Markos
authors: M
authors: Ntaoukakis
authors: Elena
authors: E
authors: Vakonaki
authors: Eleni D
authors: ED
authors: Lagoudaki
authors: Ismini
authors: I
authors: Lasithiotaki
authors: Anna
authors: A
authors: Sarchianaki
authors: Anastasios
authors: A
authors: Koutsopoulos
authors: Emmanouil K
authors: EK
authors: Symvoulakis
authors: Demetrios A
authors: DA
authors: Spandidos
authors: Katerina M
authors: KM
authors: Antoniou
authors: George
authors: G
authors: Sourvinos
mesh_terms: D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000503:physiopathology / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005541:Forced Expiratory Volume; D005838:Genotype; D006801:Humans; D008175:Lung Neoplasms / Q000503:physiopathology / Q000821:virology*; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D014797:Vital Capacity
chemical_list: D004279:DNA, Viral