title: The disease resistance response in pea is associated with increased levels of specific mRNAs.
abstract: A cDNA library was constructed using poly(A)(+)RNA fromPisum sativum which had been treated for 8 h with the fungusFusarium solani f. sp.phaseoli. Two thousand four hundred recombinant colonies were screened by differential colony hybridization using(32)P-labelled cDNAs prepared from RNA extracted from either noninoculated or inoculated pea tissue. cDNA clones were then selected, which showed greater hybridization with cDNA prepared from pea RNA 8 h post-inoculation than with a cDNA probe from 0 h. Seven distinct hybridization classes were chosen for further study. Northern blot analyses of total cellular RNAs inoculated for 16 h with eitherF. solani phaseoli or water demonstrated that each cDNA clone selected represents an mRNA species which increases substantially in abundance during infection.Results of(3)H-uridine pulse-labelling experiments suggested that enhanced synthesis is at least partially responsible for the accumulation of the fungus-inducible mRNAs which hybridized with the clones.
authors: Program in Molecular Biology, Princeton University, 99164-6430, Pullman, WA, U.S.A..
authors: R C
authors: RC
authors: Riggleman
authors: B
authors: B
authors: Fristensky
authors: L A
authors: LA
authors: Hadwiger