title: Carbohydrate-functionalized collagen matrices: design and characterization of a novel neoglycosylated biomaterial.
abstract: Collagen matrices have been neoglycosylated with lactose by reductive amination at lysine side chains. AFM analysis highlights that the chemical does not affect molecular assembly into fibrils. Moreover, ELLA biochemical assays show that the glycan moiety is efficiently exposed on the matrix surface for receptor recognition.
authors: Department of Biotechnology and Biosciences, Università degli Studi Milano-Bicocca, Piazza della Scienza 2, Milan, Italy.
authors: Laura
authors: L
authors: Russo
authors: Dipartimento di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, Piazza Leonardo da Vinci 32, Milan, Italy.
authors: Alfonso
authors: A
authors: Gautieri
authors: Dipartimento di Scienze Chirurgiche e Morfologiche, Università degli Studi dell'Insubria, Via Guicciardini 9, 21100 Varese, Italy.
authors: Mario
authors: M
authors: Raspanti
authors: Department of Nanomedicine, The Methodist Hospital Research Institute, 6670 Bertner Ave, Houston, TX 77030, USA; Pain Therapy Service, Fondazione IRCCS Policlinico San Matteo, via Golgi, 19, 27100 Pavia, Italy.
authors: Francesca
authors: F
authors: Taraballi
authors: Department of Biotechnology and Biosciences, Università degli Studi Milano-Bicocca, Piazza della Scienza 2, Milan, Italy.
authors: Francesco
authors: F
authors: Nicotra
authors: Dipartimento di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, Piazza Leonardo da Vinci 32, Milan, Italy.
authors: Simone
authors: S
authors: Vesentini
authors: Department of Biotechnology and Biosciences, Università degli Studi Milano-Bicocca, Piazza della Scienza 2, Milan, Italy. Electronic address: laura.cipolla@unimib.it.
authors: Laura
authors: L
authors: Cipolla
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002236:Carbohydrate Conformation; D003094:Collagen / Q000737:chemistry*; D015195:Drug Design; D006031:Glycosylation; D007785:Lactose / Q000737:chemistry*; D056004:Molecular Dynamics Simulation
chemical_list: D001672:Biocompatible Materials; D003094:Collagen; D007785:Lactose
keywords: AFM; Collagen; Lactose; Lectins; Neoglycosylation; Reductive amination