title: Acute total sleep deprivation potentiates amphetamine-induced locomotor-stimulant effects and behavioral sensitization in mice.
abstract: It has been demonstrated that a prolonged period (48 h) of paradoxical sleep deprivation (PSD) potentiates amphetamine (AMP)-induced behavioral sensitization, an animal model of addiction-related neuroadaptations. In the present study, we examined the effects of an acute short-term deprivation of total sleep (TSD) (6h) on AMP-induced behavioral sensitization in mice and compared them to the effects of short-term PSD (6 h). Three-month-old male C57BL/6J mice underwent TSD (experiment 1-gentle handling method) or PSD (experiment 2-multiple platforms method) for 6 h. Immediately after the sleep deprivation period, mice were tested in the open field for 10 min under the effects of saline or 2.0 mg/kg AMP. Seven days later, to assess behavioral sensitization, all of the mice received a challenge injection of 2.0 mg/kg AMP and were tested in the open field for 10 min. Total, peripheral, and central locomotion, and grooming duration were measured. TSD, but not PSD, potentiated the hyperlocomotion induced by an acute injection of AMP and this effect was due to an increased locomotion in the central squares of the apparatus. Similarly, TSD facilitated the development of AMP-induced sensitization, but only in the central locomotion parameter. The data indicate that an acute period of TSD may exacerbate the behavioral effects of AMP in mice. Because sleep architecture is composed of paradoxical and slow wave sleep, and 6-h PSD had no effects on AMP-induced hyperlocomotion or sensitization, our data suggest that the deprivation of slow wave sleep plays a critical role in the mechanisms that underlie the potentiating effects of TSD on both the acute and sensitized addiction-related responses to AMP.
authors: Department of Psychobiology, Universidade Federal de São Paulo, R. Napoleão de Barros, 925, 04024002 São Paulo, SP, Brazil.
authors: Luis P
authors: LP
authors: Saito
authors: Department of Pharmacology, Universidade Federal de São Paulo, R. Botucatu, 862, Ed. Leal Prado, 1º andar, 04023062 São Paulo, SP, Brazil; Department of Psychology, Florida State University, 1107 W. Call St, 32304 Tallahassee, FL, USA. Electronic address: danifukushiro@gmail.com.
authors: Daniela F
authors: DF
authors: Fukushiro
authors: Department of Pharmacology, Universidade Federal de São Paulo, R. Botucatu, 862, Ed. Leal Prado, 1º andar, 04023062 São Paulo, SP, Brazil.
authors: André W
authors: AW
authors: Hollais
authors: Department of Pharmacology, Universidade Federal de São Paulo, R. Botucatu, 862, Ed. Leal Prado, 1º andar, 04023062 São Paulo, SP, Brazil.
authors: Elisa
authors: E
authors: Mári-Kawamoto
authors: Department of Pharmacology, Universidade Federal de São Paulo, R. Botucatu, 862, Ed. Leal Prado, 1º andar, 04023062 São Paulo, SP, Brazil.
authors: Jacqueline M
authors: JM
authors: Costa
authors: Department of Psychobiology, Universidade Federal de São Paulo, R. Napoleão de Barros, 925, 04024002 São Paulo, SP, Brazil.
authors: Laís F
authors: LF
authors: Berro
authors: Department of Pharmacology, Universidade Federal de São Paulo, R. Botucatu, 862, Ed. Leal Prado, 1º andar, 04023062 São Paulo, SP, Brazil.
authors: Tatiana C F
authors: TC
authors: Aramini
authors: Department of Pharmacology, Universidade Federal de São Paulo, R. Botucatu, 862, Ed. Leal Prado, 1º andar, 04023062 São Paulo, SP, Brazil.
authors: Raphael
authors: R
authors: Wuo-Silva
authors: Department of Psychobiology, Universidade Federal de São Paulo, R. Napoleão de Barros, 925, 04024002 São Paulo, SP, Brazil.
authors: Monica L
authors: ML
authors: Andersen
authors: Department of Psychobiology, Universidade Federal de São Paulo, R. Napoleão de Barros, 925, 04024002 São Paulo, SP, Brazil.
authors: Sergio
authors: S
authors: Tufik
authors: Department of Psychobiology, Universidade Federal de São Paulo, R. Napoleão de Barros, 925, 04024002 São Paulo, SP, Brazil; Department of Pharmacology, Universidade Federal de São Paulo, R. Botucatu, 862, Ed. Leal Prado, 1º andar, 04023062 São Paulo, SP, Brazil.
authors: Roberto
authors: R
authors: Frussa-Filho
mesh_terms: D000662:Amphetamines / Q000494:pharmacology*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D004195:Disease Models, Animal; D008124:Locomotion / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012892:Sleep Deprivation / Q000503:physiopathology*
chemical_list: D000662:Amphetamines
keywords: Amphetamine; Behavioral sensitization; Hyperlocomotion; Mice; Paradoxical sleep deprivation; Total sleep deprivation