title: Multicenter validation of the VITEK MS v2.0 MALDI-TOF mass spectrometry system for the identification of fastidious gram-negative bacteria.
abstract: The VITEK MS v2.0 MALDI-TOF mass spectrometry system's performance in identifying fastidious gram-negative bacteria was evaluated in a multicenter study. Compared with the reference method (DNA sequencing), the VITEK MS system provided an accurate, species-level identification for 96% of 226 isolates; an additional 1% were accurately identified to the genus level.
authors: Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA. Electronic address: jbranda@partners.org.
authors: John A
authors: JA
authors: Branda
authors: Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
authors: Jenna
authors: J
authors: Rychert
authors: Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA.
authors: Carey-Ann D
authors: CA
authors: Burnham
authors: Department of Pathology and Laboratory Medicine, North Shore-LIJ Health System Laboratories, Lake Success, NY, USA.
authors: Maureen
authors: M
authors: Bythrow
authors: Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
authors: Omai B
authors: OB
authors: Garner
authors: Department of Pathology and Laboratory Medicine, North Shore-LIJ Health System Laboratories, Lake Success, NY, USA; Department of Pathology and Laboratory Medicine, Hofstra North Shore-LIJ School of Medicine, Hempstead, NY, USA.
authors: Christine C
authors: CC
authors: Ginocchio
authors: Barnes Jewish Hospital, St. Louis, MO, USA.
authors: Rebecca
authors: R
authors: Jennemann
authors: Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
authors: Michael A
authors: MA
authors: Lewinski
authors: Department of Pathology and Laboratory Medicine, North Shore-LIJ Health System Laboratories, Lake Success, NY, USA.
authors: Ryhana
authors: R
authors: Manji
authors: Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
authors: A Brian
authors: AB
authors: Mochon
authors: Department of Clinical Pathology, Cleveland Clinic, Cleveland, OH, USA.
authors: Gary W
authors: GW
authors: Procop
authors: Department of Clinical Pathology, Cleveland Clinic, Cleveland, OH, USA.
authors: Sandra S
authors: SS
authors: Richter
authors: Department of Clinical Pathology, Cleveland Clinic, Cleveland, OH, USA.
authors: Linda F
authors: LF
authors: Sercia
authors: Barnes Jewish Hospital, St. Louis, MO, USA; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA; Department of Pathology and Laboratory Medicine, Hofstra North Shore-LIJ School of Medicine, Hempstead, NY, USA.
authors: Lars F
authors: LF
authors: Westblade
authors: Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
authors: Mary Jane
authors: MJ
authors: Ferraro
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods* / Q000592:standards; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods* / Q000592:standards
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Fastidious; Gram-negative bacilli; Identification; MALDI-TOF MS; VITEK