title: Preparation of an autologous heart valve with a stent (stent-biovalve) using the stent eversion method.
abstract: We designed a novel method for constructing an autologous heart valve with a stent, called a stent-biovalve. In constructing completely autologous heart valves, named biovalves, which used in-body tissue architecture technology, tissues for leaflets were formed via ingrowths into narrow apertures in the preparation molds, frequently leading to delayed or incomplete biovalve preparation. In this technique, self-expandable nitinol stents after everting were mounted on an acrylic column-shaped part and partially covered with an acrylic cylinder-shaped part with three slits. This assembled mold was placed into subcutaneous abdominal pouches in beagles or goats for 4 weeks. Upon removing the acrylic parts after harvesting and trimming of capsulated tissues, a tubular hollow structure with three pocket-flaps of membranous tissue rigidly fixed to the stent's outer surface was obtained. Then, the stent was turned inside out to the original form, thus moving the pocket-flaps from outside to the inside. Stent-biovalves with a sufficient coaptation area were thus obtained with little tissue damage in all cases. The valve opened smoothly, and high aperture ratio was noted. This novel technique was thus highly effective in constructing a robust, completely autologous stent-biovalve with adequate valve function.
authors: Division of Medical Engineering and Materials, National Cerebral and Cardiovascular Center Research Institute, Osaka, Japan; Department of Veterinary Medicine, Veterinary Cardiovascular Medicine and Surgery Unit, Laboratory of Veterinary Internal Medicine, College of Bioresource Sciences, Nihon University, Kanagawa, Japan.
authors: Takeshi
authors: T
authors: Mizuno
authors: Yoshiaki
authors: Y
authors: Takewa
authors: Hirohito
authors: H
authors: Sumikura
authors: Kentaro
authors: K
authors: Ohnuma
authors: Takeshi
authors: T
authors: Moriwaki
authors: Masashi
authors: M
authors: Yamanami
authors: Tomonori
authors: T
authors: Oie
authors: Eisuke
authors: E
authors: Tatsumi
authors: Masami
authors: M
authors: Uechi
authors: Yasuhide
authors: Y
authors: Nakayama
mesh_terms: D000497:Alloys; D000818:Animals; D064592:Autografts; D001705:Bioprosthesis; D004285:Dogs; D005260:Female; D006350:Heart Valve Prosthesis; D006351:Heart Valves / Q000378:metabolism* / Q000473:pathology / Q000637:transplantation*; D008297:Male; D015607:Stents
chemical_list: D000497:Alloys; C013616:nitinol
keywords: autologous tissue; biovalve; heart valve; stent