title: Peritoneal cell sheets composed of mesothelial cells and fibroblasts prevent intra-abdominal adhesion formation in a rat model.
abstract: Postoperative intra-abdominal adhesions remain an unsolved problem despite significant progress in the surgical procedures themselves. They often lead to small-bowel obstruction, chronic abdominal and pelvic pain, as well as female infertility. The loss of mesothelial cells and several components of the inflammatory system following injury to the peritoneum results in fibrin formation and angiogenesis. The remaining fibrin matrix and angiogenesis lead to replacement by fibroblasts and fibrous band formation. The aim of this study was to develop a new therapeutic method of preventing intra-abdominal adhesions. We fabricated transplantable peritoneal cell sheets from the rat peritoneum by cell sheet engineering using a temperature-responsive culture system. The peritoneal cell sheets developed were composed of an upper monolayer of mesothelial cells and underlying multilayered fibroblasts, similar to the peritoneum in vivo. Transplantation of peritoneal cell sheets prevented tissue adhesion, fibrin deposition and angiogenesis, and, moreover, lymphangiogenesis and macrophage infiltration in a rat caecum cauterization adhesion model. Copyright © 2013 John Wiley & Sons, Ltd.
authors: Department of Medicine, Kidney Center, Tokyo Women's Medical University, Tokyo, Japan.
authors: Kunio
authors: K
authors: Kawanishi
authors: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.
authors: Masayuki
authors: M
authors: Yamato
authors: Department of Clinical Engineering, Tokyo Women's Medical University, Tokyo, Japan.
authors: Ryouichi
authors: R
authors: Sakiyama
authors: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan. tokano@abmes.twmu.ac.jp.
authors: Teruo
authors: T
authors: Okano
authors: Department of Medicine, Kidney Center, Tokyo Women's Medical University, Tokyo, Japan.
authors: Kosaku
authors: K
authors: Nitta
mesh_terms: D000818:Animals; D004848:Epithelium / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D008297:Male; D010537:Peritoneum / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D000267:Tissue Adhesions / Q000378:metabolism / Q000517:prevention & control*
keywords: abdominal adhesion; cell sheet; fibroblasts; mesothelial cells; scaffold-free tissue engineering; temperature-responsive culture system