title: Effect of leukemia inhibitory factor and forskolin on establishment of rat embryonic stem cell lines.
abstract: This study was designed to investigate whether supplementation of 2i medium with leukemia inhibitory factor (LIF) and/or forskolin would support establishment of germline-competent rat embryonic stem (ES) cell lines. Due to the higher likelihood of outgrowth rates, supplementation of forskolin with or without LIF contributed to the higher establishment efficiency of ES cell lines in the WDB strain. Germline transmission competency of the chimeric rats was not influenced by the profile of ES cell lines until their establishment. When the LIF/forskolin-supplemented 2i medium was used, the rat strain used as the blastocyst donor, such as the WI strain, was a possible factor negatively influencing the establishment efficiency of ES cell lines. Once ES cell lines were established, all lines were found to be germline-competent by a progeny test in chimeric rats. In conclusion, both LIF and forskolin are not essential but can play a beneficial role in the establishment of "genuine" rat ES cell lines.
authors: Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Aichi 444-8787, Japan.
authors: Masumi
authors: M
authors: Hirabayashi
authors: Teppei
authors: T
authors: Goto
authors: Chihiro
authors: C
authors: Tamura
authors: Makoto
authors: M
authors: Sanbo
authors: Hiromasa
authors: H
authors: Hara
authors: Shinichi
authors: S
authors: Hochi
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology*; D053595:Embryonic Stem Cells / Q000187:drug effects*; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology*; D051381:Rats
chemical_list: D053605:Leukemia Inhibitory Factor; D005576:Colforsin