title: EcoliOverExpressionDB: a database of recombinant protein overexpression in E. coli.
abstract: Recombinant protein production is a significant biotechnological process as it allows researchers to produce a specific protein in desired quantities. Escherichia coli (E. coli) is the most popular heterologous expression host for the production of recombinant proteins due to its advantages such as low cost, high-productivity, well-characterized genetics, simple growth requirements and rapid growth. There are a number of factors that influence the expression level of a recombinant protein in E. coli which are the gene to be expressed, the expression vector, the expression host, and the culture condition. The major motivation to develop our database, EcoliOverExpressionDB, is to provide a means for researchers to quickly locate key factors in the overexpression of certain proteins. Such information would be a useful guide for the overexpression of similar proteins in E. coli. To the best of the present researchers' knowledge, in general and specifically in E. coli, EcoliOverExpressionDB is the first database of recombinant protein expression experiments which gathers the influential parameters on protein overexpression and the results in one place.
  
  
  BACKGROUND
  EcoliOverExpressionDB is freely available and accessible using all major browsers at http://birg4.fbb.utm.my:8080/EcoliOverExpressionDB/.
authors: Soft Computing Research Group, Faculty of Computing, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia. Electronic address: hnarjeskhatoon2@utm.live.my.
authors: Narjeskhatoon
authors: N
authors: Habibi
authors: School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia; Advanced Medical and Dental Institute, Universiti Sains Malaysia, 11800 Penang, Malaysia; Centre for Chemical Biology, Universiti Sains Malaysia, 11800 Penang, Malaysia.
authors: Mohd Razip
authors: MR
authors: Samian
authors: Soft Computing Research Group, Faculty of Computing, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia.
authors: Siti Zaiton Mohd
authors: SZ
authors: Hashim
authors: ViCube Lab, Faculty of Computing, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia.
authors: Alireza
authors: A
authors: Norouzi
mesh_terms: D001709:Biotechnology; D016208:Databases, Factual; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D020407:Internet; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins
keywords: Database; Escherichia coli; Overexpression; Recombinant protein; Recombinant protein overexpression