title: Sensing acetylcholine and anticholinesterase compounds.
abstract: Acetylcholine is a key neurotransmitter, and anticholinesterase agents are essential compounds used as medical drugs, pesticides, and chemical warfare agents. A semisynthetic fluorescence-based probe for the direct, real-time detection of acetylcholine and anticholinesterase compounds is introduced. The probe possesses good sensitivity, tunable detection range, and can be selectively targeted to cell surfaces, thereby making it an attractive tool for applications in analytical chemistry and quantitative biology.
authors: Ecole Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, National Centre of Competence in Research (NCCR) in Chemical Biology, 1015 Lausanne (Switzerland).
authors: Alberto
authors: A
authors: Schena
authors: Kai
authors: K
authors: Johnsson
mesh_terms: D000109:Acetylcholine / Q000032:analysis*; D000110:Acetylcholinesterase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015374:Biosensing Techniques; D002619:Chemical Warfare Agents / Q000032:analysis; D002800:Cholinesterase Inhibitors / Q000032:analysis; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D057809:HEK293 Cells; D006801:Humans; D018613:Microscopy, Confocal; D010575:Pesticides / Q000032:analysis
chemical_list: D002619:Chemical Warfare Agents; D002800:Cholinesterase Inhibitors; D005456:Fluorescent Dyes; D010575:Pesticides; D000110:Acetylcholinesterase; D000109:Acetylcholine
keywords: FRET; acetylcholine; acetylcholinesterase; biosensors; imaging