title: Characteristics of new monomolecular chimeric T-cell receptors to carcinoembryonic antigen.
abstract: We described two original genetic constructs encoding chimeric monomolecular T-cell receptors, where the effector T-cell receptor fragment was linked with the antigen-recognizing part consisting of two variable fragments of two different antibodies to carcinoembryonic antigen. Following transfection, these receptors were expressed on the cell surface and bound carcinoembryonic antigen. Human peripheral blood lymphocytes transfected with the above constructs demonstrated high cytotoxic activity against HCT116 cells expressing carcinoembryonic antigen.
authors: Russian Scientific Center of Roentgenoradiology, Ministry of Health of the Russian Federation; N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation; XEMA Company, Moscow, Russia. a.shkoporov@gmail.com.
authors: V K
authors: VK
authors: Bozhenko
authors: E I
authors: EI
authors: Shramova
authors: A M
authors: AM
authors: Shishkin
authors: A V
authors: AV
authors: Ivanov
authors: E V
authors: EV
authors: Khokhlova
authors: Yu S
authors: YS
authors: Lebedin
authors: A N
authors: AN
authors: Shkoporov
mesh_terms: D002272:Carcinoembryonic Antigen / Q000276:immunology*; D002470:Cell Survival; D003602:Cytotoxicity, Immunologic; D045325:HCT116 Cells; D057809:HEK293 Cells; D019073:HT29 Cells; D006801:Humans; D007167:Immunotherapy; D009369:Neoplasms / Q000276:immunology / Q000628:therapy; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D002272:Carcinoembryonic Antigen; D011948:Receptors, Antigen, T-Cell; D011993:Recombinant Fusion Proteins