title: An efficient protocol for total DNA extraction from the members of order Zingiberales- suitable for diverse PCR based downstream applications.
abstract: Different protocols are usually used for extracting total deoxyribonucleic acid (DNA) from different plant species of same order and DNA of the associated viruses. Here, we describe a rapid, efficient and universal protocol for isolating total DNA from the members of Zingiberales which harbor a high amount of polysaccharides and secondary metabolites. DNA isolated with this protocol was successfully used for PCR based downstream applications viz. random amplified polymorphic DNA (RAPD), Inter-simple sequence repeats (ISSR), DNA barcoding gene (Internal transcribed spacer and trnl-f) amplification and detection of the viruses.
authors.affiliation: Medicinal Plants & Horticultural Resources Division, Institute of Bioresources and Sustainable Development, Takyelpat Institutional Area, Imphal, 795001 Manipur India.
authors.forename: Khumallambam Devala
authors.initials: KD
authors.lastname: Devi
authors.affiliation: Medicinal Plants & Horticultural Resources Division, Institute of Bioresources and Sustainable Development, Takyelpat Institutional Area, Imphal, 795001 Manipur India.
authors.forename: Kshetrimayum
authors.initials: K
authors.lastname: Punyarani
authors.affiliation: Medicinal Plants & Horticultural Resources Division, Institute of Bioresources and Sustainable Development, Takyelpat Institutional Area, Imphal, 795001 Manipur India.
authors.forename: Nandeibam Samarjit
authors.initials: NS
authors.lastname: Singh
authors.affiliation: Medicinal Plants & Horticultural Resources Division, Institute of Bioresources and Sustainable Development, Takyelpat Institutional Area, Imphal, 795001 Manipur India.
authors.forename: Huidrom Sunitibala
authors.initials: HS
authors.lastname: Devi
keywords: CTAB; DNA barcoding gene; DNA extraction; ISSR; RAPD; Virus detection; Zingiberales