title: Structural behaviour in condensed bovine serum albumin systems following application of high pressure.
abstract: The present study shows that application of high hydrostatic pressure of 600MPa for 15min at ambient temperature on condensed bovine serum albumin systems (BSA) with up to 80% w/w solids content has a limited effect on the conformational structure of the protein, as compared to thermal treatment. This was demonstrated throughout the experimental concentration range using small-deformation dynamic oscillation, differential scanning calorimetry and infrared spectroscopy. BSA possesses seventeen disulfide linkages per molecule, which constitutes a stable arrangement with high energy requirements for substantial structure alteration. Upon cooling, pressurised materials undergo vitrification and networks exhibit comparative mechanical strength to that of thermally treated counterparts. The mechanical manifestation of the glass transition region and glassy state for atmospheric and pressurised samples was examined by the method of reduced variables and the combined framework of WLF/free volume theory producing disparate predictions of the glass transition temperature for the two types of polymeric network.
authors.affiliation: School of Applied Sciences, RMIT University, City Campus, Melbourne, Vic. 3001, Australia.
authors.forename: Sobhan
authors.initials: S
authors.lastname: Savadkoohi
authors.affiliation: School of Applied Sciences, RMIT University, City Campus, Melbourne, Vic. 3001, Australia.
authors.forename: Anna
authors.initials: A
authors.lastname: Bannikova
authors.affiliation: School of Applied Sciences, RMIT University, City Campus, Melbourne, Vic. 3001, Australia. Electronic address: stefan.kasapis@rmit.edu.au.
authors.forename: Stefan
authors.initials: S
authors.lastname: Kasapis
authors.affiliation: School of Health Sciences, University of Ballarat, Mt. Helen Campus, Ballarat, Vic. 3353, Australia.
authors.forename: Benu
authors.initials: B
authors.lastname: Adhikari
mesh_terms: D000818:Animals; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D006874:Hydrostatic Pressure; D011487:Protein Conformation; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D044366:Transition Temperature
chemical_list: D012710:Serum Albumin, Bovine
keywords: Bovine serum albumin; FTIR; High hydrostatic pressure; High-solid systems; Mechanical T(g); MicroDSC