title: In vivo characterization of dynein-driven nanovectors using Drosophila oocytes.
abstract: Molecular motors transport various cargoes including vesicles, proteins and mRNAs, to distinct intracellular compartments. A significant challenge in the field of nanotechnology is to improve drug nuclear delivery by engineering nanocarriers transported by cytoskeletal motors. However, suitable in vivo models to assay transport and delivery efficiency remain very limited. Here, we develop a fast and genetically tractable assay to test the efficiency and dynamics of fluospheres (FS) using microinjection into Drosophila oocytes coupled with time-lapse microscopy. We designed dynein motor driven FS using a collection of dynein light chain 8 (LC8) peptide binding motifs as molecular linkers and characterized in real time the efficiency of the FS movement according to its linker's sequence. Results show that the conserved LC8 binding motif allows fast perinuclear nanoparticle's accumulation in a microtubule and dynein dependent mechanism. These data reveal the Drosophila oocyte as a new valuable tool for the design of motor driven nanovectors.
authors.affiliation: University of Nice Sophia-Antipolis, Institute of Biology Valrose (iBV), UMR 7277-CNRS, UMR 1091 INSERM, Nice, France.
authors.forename: Nadège
authors.initials: N
authors.lastname: Parassol
authors.affiliation: Université de Nice Sophia-Antipolis, Institut de Chimie de Nice (ICN), UMR 7272-CNRS, Nice, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Bienvenu
authors.affiliation: Université de Nice Sophia-Antipolis, Institut de Chimie de Nice (ICN), UMR 7272-CNRS, Nice, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Boglio
authors.affiliation: Université de Nice Sophia-Antipolis, Institut de Chimie de Nice (ICN), UMR 7272-CNRS, Nice, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Fiorucci
authors.affiliation: University of Nice Sophia-Antipolis, Institute of Biology Valrose (iBV), UMR 7277-CNRS, UMR 1091 INSERM, Nice, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Cerezo
authors.affiliation: Université de Nice Sophia-Antipolis, Institut de Chimie de Nice (ICN), UMR 7272-CNRS, Nice, France.
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Yu
authors.affiliation: Université de Nice Sophia-Antipolis, Institut de Chimie de Nice (ICN), UMR 7272-CNRS, Nice, France.
authors.forename: Guilhem
authors.initials: G
authors.lastname: Godeau
authors.affiliation: Université de Nice Sophia-Antipolis, Institut de Chimie de Nice (ICN), UMR 7272-CNRS, Nice, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Greiner
authors.affiliation: Université de Nice Sophia-Antipolis, Institut de Chimie de Nice (ICN), UMR 7272-CNRS, Nice, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Vierling
authors.affiliation: University of Nice Sophia-Antipolis, Institute of Biology Valrose (iBV), UMR 7277-CNRS, UMR 1091 INSERM, Nice, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Noselli
authors.affiliation: Université de Nice Sophia-Antipolis, Institut de Chimie de Nice (ICN), UMR 7272-CNRS, Nice, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Di Giorgio
authors.affiliation: University of Nice Sophia-Antipolis, Institute of Biology Valrose (iBV), UMR 7277-CNRS, UMR 1091 INSERM, Nice, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Van De Bor
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D003599:Cytoskeleton / Q000378:metabolism*; D029721:Drosophila Proteins / Q000737:chemistry* / Q000378:metabolism; D004331:Drosophila melanogaster; D016503:Drug Delivery Systems; D004398:Dyneins / Q000737:chemistry* / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D004398:Dyneins