title: Analyses of mineral specific surface area and hydroxyl substitution for intact bone.
abstract: Bone minerals possess two primary hydrogen sources: hydroxide ions in the nanocrystalline core and structural water in the amorphous surface layer. In order to accurately measure their concentrations using hydrogen to phosphorus cross polarization NMR spectroscopy, it is necessary to analyze the dependence of signal intensities on serial contact times, namely, cross polarization kinetics. A reliable protocol is developed to iteratively decompose the severely overlapped spectra and to analyze the cross-polarization kinetics, leading to measurement of hydroxyl and structural water concentrations. Structural water concentration is used to estimate mineral specific surface area and nanocrystal thickness for intact bone.
authors.affiliation: Department of Physics, Oklahoma State University, Stillwater, OK, USA 74078.
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: Taylor
authors.affiliation: Department of Nutritional Sciences, Oklahoma State University, Stillwater, OK, USA 74078.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Rendina
authors.affiliation: Department of Nutritional Sciences, Oklahoma State University, Stillwater, OK, USA 74078.
authors.forename: Brenda J
authors.initials: BJ
authors.lastname: Smith
authors.affiliation: Department of Physics, Oklahoma State University, Stillwater, OK, USA 74078.
authors.forename: Donghua H
authors.initials: DH
authors.lastname: Zhou
keywords: CP kinetics; biominerals; bone; cross-polarization; magic-angle spinning; solid-state NMR; spectral deconvolution