title: A nonthoracotomy myocardial infarction model in an ovine using autologous platelets.
abstract: OBJECTIVE
  There is a paucity of a biological large animal model of myocardial infarction (MI). We hypothesized that, using autologous-aggregated platelets, we could create an ovine model that was reproducible and more closely mimicked the pathophysiology of MI.
  
  
  METHODS
  Mepacrine stained autologous platelets from male sheep (n = 7) were used to create a myocardial infarction via catheter injection into the mid-left anterior descending (LAD) coronary artery. Serial daily serum troponin measurements were taken and tissue harvested on post-embolization day three. Immunofluorescence microscopy was used to detect the mepacrine-stained platelet-induced thrombus, and histology performed to identify three distinct myocardial (infarct, peri-ischemic "border zone," and remote) zones.
  
  
  RESULTS
  Serial serum troponin levels (μg/mL) measured 0.0 ± 0.0 at baseline and peaked at 297.4 ± 58.0 on post-embolization day 1, followed by 153.0 ± 38.8 on day 2 and 76.7 ± 19.8 on day 3. Staining confirmed distinct myocardial regions of inflammation and fibrosis as well as mepacrine-stained platelets as the cause of intravascular thrombosis.
  
  
  CONCLUSIONS
  We report a reproducible, unique model of a biological myocardial infarction in a large animal model. This technique can be used to study acute, regional myocardial changes following a thrombotic injury.
authors.affiliation: The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA.
authors.forename: Tyler
authors.initials: T
authors.lastname: Spata
authors.affiliation: The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Bobek
authors.affiliation: The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA.
authors.forename: Bryan A
authors.initials: BA
authors.lastname: Whitson
authors.affiliation: The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA.
authors.forename: Sampath
authors.initials: S
authors.lastname: Parthasarathy
authors.affiliation: The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Mohler
authors.affiliation: The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA.
authors.forename: Robert S D
authors.initials: RS
authors.lastname: Higgins
authors.affiliation: The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA ; Division of Cardiac Surgery, Department of Surgery, The Ohio State University Wexner Medical Center, 410 W. 10th Avenue, N-831 Doan Hall, Columbus, OH 43210, USA.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Kilic
mesh_terms: D000818:Animals; D004396:Coloring Agents / Q000737:chemistry; D003331:Coronary Vessels / Q000473:pathology*; D004195:Disease Models, Animal; D008297:Male; D008856:Microscopy, Fluorescence; D009203:Myocardial Infarction / Q000097:blood / Q000139:chemically induced / Q000503:physiopathology*; D017713:Platelet Transfusion; D011796:Quinacrine / Q000737:chemistry*; D012756:Sheep; D013908:Thoracotomy / Q000379:methods; D013927:Thrombosis / Q000473:pathology; D014336:Troponin / Q000097:blood
chemical_list: D004396:Coloring Agents; D014336:Troponin; D011796:Quinacrine