title: Combined use of the BinaxNOW Staphylococcus aureus test with the Clearview PBP2a assay for the early detection of methicillin-resistant S. aureus from positive blood cultures.
abstract: The use of combination 2 novel immunochromatographic assays for same-day detection of methicillin-resistant Staphylococcus aureus (MRSA) from positive blood cultures was evaluated. Compared to the standard culture methods, the BinaxNOW® S. aureus test demonstrated 98.7% sensitivity and 100% specificity in correctly identifying S. aureus. The sensitivity and specificity of the Clearview® PBP2a assay in differentiating MRSA from methicillin-susceptible S. aureus were 97.1% and 100%.
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang, PR China.
authors.forename: Haishen
authors.initials: H
authors.lastname: Kong
authors.affiliation: Center of Clinical Laboratory, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Tong
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang, PR China.
authors.forename: Weili
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Center of Clinical Laboratory, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, PR China.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Fu
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang, PR China; Center of Clinical Laboratory, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, PR China. Electronic address: zylixf@126.com.
authors.forename: Xuefen
authors.initials: X
authors.lastname: Li
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D042241:Early Diagnosis; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000302:isolation & purification*
chemical_list: D003470:Culture Media
keywords: Bacteremia; Blood culture; Identification; Immunochromatographic assay; Methicillin-resistant Staphylococcus aureus