title: Expression of drebrin, an actin binding protein, in basal cell carcinoma, trichoblastoma and trichoepithelioma.
abstract: Drebrin, an F-actin binding protein, is known to play important roles in cell migration, synaptogenesis and neural plasticity. Although drebrin was long thought to be specific for neuronal cells, its expression has recently been reported in non-neuronal cells. As for skin-derived cells, drebrin was shown to be enriched at adhering junctions (AJs) in cultured primary keratinocytes and also be highly expressed in basal cell carcinoma (BCC) cells. Since BCC and two types of benign neoplasm, trichoblastoma and trichoepithelioma, are considered to derive from the same origin, follicular germinative cells, it is sometimes difficult to morphologically distinguish BCC from trichoblastoma and trichoepithelioma. In this study, we performed immunohistochemical staining of drebrin in BCC, trichoblastoma and trichoepithelioma, to examine whether drebrin could serve as a biomarker for BCC diagnosis. In western blotting, drebrin was detected highly and moderately in the lysates from a squamous cell carcinoma cell line, DJM-1, and normal human epidermis, respectively. In immunofluorescence analyses, drebrin was colocalized with markers of AJs and tight junctions in DJM-1 cells and detected at cell-cell junction areas of human normal epidermis tissue. We then examined the distribution patterns of drebrin in BCC, trichoblastoma and trichoepithelioma. In BCC tissues, intense and homogeneous drebrin expression was observed mainly at tumor cell-cell boundaries. In contrast, drebrin was stained only weakly and non-homogeneously in trichoblastoma and trichoepthelioma tissue samples. For differential diagnosis of BCC, drebrin may be a novel and useful marker.
authors.affiliation: Department of Dermatology, Gifu University Graduate School of Medicine, Gifu, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Mizutani
authors.affiliation: Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, Kasugai, Japan.
authors.forename: Ikuko
authors.initials: I
authors.lastname: Iwamoto
authors.affiliation: Department of Dermatology, Gifu University Graduate School of Medicine, Gifu, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kanoh
authors.affiliation: Department of Dermatology, Gifu University Graduate School of Medicine, Gifu, Japan. marikoseishima@yahoo.co.jp.
authors.forename: Mariko
authors.initials: M
authors.lastname: Seishima
authors.affiliation: Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, Kasugai, Japan. knagata@inst-hsc.jp.
authors.forename: Koh-ichi
authors.initials: K
authors.lastname: Nagata
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D002280:Basal Cell Carcinoma / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D003937:Diagnosis, Differential; D006801:Humans; D051379:Mice; D009479:Neuropeptides / Q000378:metabolism*; D051381:Rats; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D012878:Skin Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D009479:Neuropeptides; C053386:drebrins