title: Purification and characterization of tannin acyl hydrolase produced by mixed solid state fermentation of wheat bran and marigold flower by Penicillium notatum NCIM 923.
abstract: Tannin acyl hydrolase produced extracellularly by the fungal strain Penicillium notatum NCIM 923 in mixed solid state fermentation of wheat bran and marigold flower in the ratio 4 : 1 was purified from the cell-free extract broth by ammonium sulphate fractionation followed by diethylaminoethyl-cellulose column chromatography. Tannase was purified by 19.89-fold with yield of 11.77%. The specific activity of crude tannase was found to be 1.31 U/mg protein while that of purified tannase was 22.48 U/mg protein. SDS-PAGE analysis indicated that the enzyme is dimeric with one major band of molecular mass 97 kDa and a very light band of molecular mass 43 kDa. Temperature of 35 to 40°C and pH 5 were optimum for tannase activity. The enzyme retained more than 60% of its stability at 60°C and 40% stability at pH 3 and 8, respectively. K m was found to be 0.33 × 10(-2) M and V max = 40 U/mg. Since the enzyme is active over a wide range of pH and temperature, it could find potential use in the food processing industry.
authors.affiliation: Department of Microbiology, Vijaygarh Jyotish Ray College, Kolkata 700032, West Bengal, India.
authors.forename: Saswati
authors.initials: S
authors.lastname: Gayen
authors.affiliation: Department of Food Technology and Biochemical Engineering, Jadavpur University, Kolkata 700032, West Bengal, India.
authors.forename: Uma
authors.initials: U
authors.lastname: Ghosh
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000302:isolation & purification* / Q000652:urine*; D004043:Dietary Fiber / Q000378:metabolism*; D005285:Fermentation / Q000502:physiology*; D035264:Flowers / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010408:Penicillium chrysogenum / Q000378:metabolism*; D013696:Temperature
chemical_list: D004043:Dietary Fiber; D002265:Carboxylic Ester Hydrolases; C022711:tannase