title: Treg depletion followed by intracerebral CpG-ODN injection induce brain tumor rejection.
abstract: Using brain lymphoma model, we demonstrate that immunotherapy combining Treg depletion (using anti-CD25 mAb PC61) followed by intracranial CpG-ODN administration induced tumor rejection in all treated mice and led to the establishment of a memory antitumor immune response in 60% of them. This protective effect was associated with a recruitment of NK cells and, to a lesser extent, of dendritic cells, B cells and T lymphocytes. NK cell depletion abolished the protective effect of the treatment, confirming a major role of NK cells in brain tumor elimination. Each treatment used alone failed to protect brain tumor bearing mice, revealing the therapeutic benefit of combining Treg depletion and local CpG-ODN injection.
authors.affiliation: Université d'Angers, Angers, France; INSERM, UMR892, Angers, France; CNRS, UMR 6299, Angers, France.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Jarry
authors.affiliation: Université d'Angers, Angers, France; INSERM, UMR892, Angers, France.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Donnou
authors.affiliation: Université d'Angers, Angers, France; INSERM, UMR892, Angers, France; CNRS, UMR 6299, Angers, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Vincent
authors.affiliation: Université d'Angers, Angers, France; INSERM, UMR892, Angers, France; CNRS, UMR 6299, Angers, France; CHU d'Angers, Laboratoire d'Immunologie et Allergologie, Angers, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Jeannin
authors.affiliation: Université d'Angers, Angers, France; INSERM, UMR892, Angers, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Pineau
authors.affiliation: Université d'Angers, Angers, France; INSERM, UMR892, Angers, France; CNRS, UMR 6299, Angers, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Fremaux
authors.affiliation: Université d'Angers, Angers, France; INSERM, UMR892, Angers, France; CNRS, UMR 6299, Angers, France; CHU d'Angers, Laboratoire d'Immunologie et Allergologie, Angers, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Delneste
authors.affiliation: Université d'Angers, Angers, France; INSERM, UMR892, Angers, France; CNRS, UMR 6299, Angers, France. Electronic address: dominique.couez@univ-angers.fr.
authors.forename: Dominique
authors.initials: D
authors.lastname: Couez
mesh_terms: D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000818:Animals; D000906:Antibodies / Q000633:toxicity; D000950:Antigens, Ly / Q000276:immunology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use; D001932:Brain Neoplasms / Q000150:complications / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005047:Etoposide / Q000494:pharmacology / Q000627:therapeutic use; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D007694:Killer Cells, Natural / Q000187:drug effects / Q000502:physiology; D008223:Lymphoma / Q000150:complications / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008569:Memory Disorders / Q000209:etiology / Q000517:prevention & control; D051379:Mice; D008810:Mice, Inbred C57BL; D055651:NK Cell Lectin-Like Receptor Subfamily B / Q000276:immunology; D009368:Neoplasm Transplantation; D009838:Oligodeoxyribonucleotides / Q000627:therapeutic use*; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors; D051217:Toll-Like Receptor 9 / Q000378:metabolism
chemical_list: D000276:Adjuvants, Immunologic; D000906:Antibodies; D000950:Antigens, Ly; D000972:Antineoplastic Agents, Phytogenic; C408982:CPG-oligonucleotide; D016207:Cytokines; D053645:Interleukin-2 Receptor alpha Subunit; C527211:Klrb1c protein, mouse; D055651:NK Cell Lectin-Like Receptor Subfamily B; D009838:Oligodeoxyribonucleotides; D051217:Toll-Like Receptor 9; D005047:Etoposide
keywords: CNS tumor; CpG-ODN; NK cells; PAMP; PRR; Treg; pathogen recognition receptor; pathogen-associated molecular pattern