title: Epigallocatechin gallate inhibits the growth of human lung cancer by directly targeting the EGFR signaling pathway.
abstract: Epigallocatechin gallate (EGCG), the major biologically active compound in green tea, is a well-known chemoprevention agent. Although several reports have shown that EGCG exerts its anticancer activity by targeting specific cell signaling pathways, the underlying molecular mechanism(s) are only partially understood. In the present study, we report that EGCG had a profound antiproliferative effect on human lung cancer cells. EGCG inhibited anchorage-independent growth and induced cell cycle G0/G1 phase arrest. The mechanism underlying EGCG antitumor potency was mainly dependent on suppression of the EGFR signaling pathway. Short-term EGCG exposure substantially decreased EGF-induced EGFR, AKT and ERK1/2 activation. Moreover, long-term EGCG treatment not only inhibited total and membranous EGFR expression, but also markedly attenuated EGFR nuclear localization and expression of the downstream target gene cyclin D1, indicating that EGCG treatment suppressed EGFR transactivation. Additionally, knockdown of EGFR in lung cancer cells decreased their sensitivity to EGCG. Thus, inhibition of the EGFR signaling pathway may partly contribute to the anticancer activity of EGCG.
authors.affiliation: Department of Cardiothoracic Surgery, The Third Xiangya Hospital, Central South University, Changsha 410013, P.R. China.
authors.forename: Yu-Chao
authors.initials: YC
authors.lastname: Ma
authors.affiliation: Key Laboratory of Cancer Proteomics of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, P.R. China.
authors.forename: Cui
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Ultrasound, The Third Xiangya Hospital, Central South University, Changsha 410013, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.affiliation: Key Laboratory of Cancer Proteomics of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Cardiothoracic Surgery, The Third Xiangya Hospital, Central South University, Changsha 410013, P.R. China.
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Jiang
authors.affiliation: Department of Cardiothoracic Surgery, The Third Xiangya Hospital, Central South University, Changsha 410013, P.R. China.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Liu
authors.affiliation: Department of Cardiothoracic Surgery, The Third Xiangya Hospital, Central South University, Changsha 410013, P.R. China.
authors.forename: Long-Yu
authors.initials: LY
authors.lastname: Jin
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D019938:Cyclin D1 / Q000378:metabolism; D019008:Drug Resistance, Neoplasm; D004354:Drug Screening Assays, Antitumor; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D055785:Gene Knockdown Techniques; D006801:Humans; D008175:Lung Neoplasms; D015398:Signal Transduction
chemical_list: D000970:Antineoplastic Agents; C530104:CCND1 protein, human; D019938:Cyclin D1; D002392:Catechin; C045651:epigallocatechin gallate; C512478:EGFR protein, human; D066246:ErbB Receptors