title: A case of Exophiala spinifera infection in Southern Brazil: Molecular identification and antifungal susceptibility.
abstract: We report a case of an 80-year-old Brazilian man, farmer, with lesions on the dorsum of the hand. A direct mycological examination, cultivation and microculture slide observation was performed. The sequencing of ITS1-5.8S rDNA-ITS2 region was carried out and the etiological agent confirmed as Exophiala spinifera. The in vitro susceptibility of this isolate to antifungal agents alone and in combination was evaluated. This is the third case of phaeohyphomycosis caused by Exophiala spinifera in Brazil.
authors.affiliation: Graduate Program in Medicine: Medical Sciences, Universidade Federal do Rio Grande do Sul. Rua Ramiro Barcelos, 2400 - 2 floor, Porto Alegre, CEP: 90035-003, Brazil ; Department of Microbiology, ICBS, Universidade Federal do Rio Grande do Sul. Rua Sarmento Leite, 500, Sala 210, Porto Alegre, CEP: 90050-170, Brazil.
authors.forename: Tatiane Caroline
authors.initials: TC
authors.lastname: Daboit
authors.affiliation: Dermatology Service, Santa Casa de Misericórdia de Porto Alegre Hospital Complex. Rua Prof. Annes Dias, 285, Porto Alegre, CEP: 90020-090, Brazil.
authors.forename: Rodrigo Pereira
authors.initials: RP
authors.lastname: Duquia
authors.affiliation: Graduate Program in Medicine: Medical Sciences, Universidade Federal do Rio Grande do Sul. Rua Ramiro Barcelos, 2400 - 2 floor, Porto Alegre, CEP: 90035-003, Brazil ; Department of Microbiology, ICBS, Universidade Federal do Rio Grande do Sul. Rua Sarmento Leite, 500, Sala 210, Porto Alegre, CEP: 90050-170, Brazil.
authors.forename: Cibele Massotti
authors.initials: CM
authors.lastname: Magagnin
authors.affiliation: Department of Microbiology, ICBS, Universidade Federal do Rio Grande do Sul. Rua Sarmento Leite, 500, Sala 210, Porto Alegre, CEP: 90050-170, Brazil.
authors.forename: Sandra Denise Camargo
authors.initials: SD
authors.lastname: Mendes
authors.affiliation: Department of Microbiology, ICBS, Universidade Federal do Rio Grande do Sul. Rua Sarmento Leite, 500, Sala 210, Porto Alegre, CEP: 90050-170, Brazil.
authors.forename: Mauricio Ramírez
authors.initials: MR
authors.lastname: Castrillón
authors.affiliation: Dermatology Service, Santa Casa de Misericórdia de Porto Alegre Hospital Complex. Rua Prof. Annes Dias, 285, Porto Alegre, CEP: 90020-090, Brazil.
authors.forename: Raquel
authors.initials: R
authors.lastname: Steglich
authors.affiliation: Dermatology Service, Santa Casa de Misericórdia de Porto Alegre Hospital Complex. Rua Prof. Annes Dias, 285, Porto Alegre, CEP: 90020-090, Brazil.
authors.forename: Inajara Silveira
authors.initials: IS
authors.lastname: Dos Santos
authors.affiliation: Dermatology Service, Santa Casa de Misericórdia de Porto Alegre Hospital Complex. Rua Prof. Annes Dias, 285, Porto Alegre, CEP: 90020-090, Brazil.
authors.forename: Gerson
authors.initials: G
authors.lastname: Vettorato
authors.affiliation: Department of Microbiology, ICBS, Universidade Federal do Rio Grande do Sul. Rua Sarmento Leite, 500, Sala 210, Porto Alegre, CEP: 90050-170, Brazil.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Valente
authors.affiliation: Graduate Program in Medicine: Medical Sciences, Universidade Federal do Rio Grande do Sul. Rua Ramiro Barcelos, 2400 - 2 floor, Porto Alegre, CEP: 90035-003, Brazil ; Department of Microbiology, ICBS, Universidade Federal do Rio Grande do Sul. Rua Sarmento Leite, 500, Sala 210, Porto Alegre, CEP: 90050-170, Brazil.
authors.forename: Maria Lúcia
authors.initials: ML
authors.lastname: Scroferneker
keywords: Checkerboard technique; Exophila spinifera; ITS rDNA; In vitro antifungal susceptibility; Phaeohyphomycosis