title: Cost effective production of pullulan from agri-industrial residues using response surface methodology.
abstract: Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components. A second order polynomial model was obtained using central composite design to understand the effects of interactions among these substrates on pullulan biosynthesis. Results indicated that, lower concentrations of CSL and DOJSC and higher concentrations of jaggery favoured pullulan production. The optimal nutrient composition (18% jaggery, 3% DOJSC and 0.97% CSL) as suggested by the model resulted in production of 66.25 g/L pullulan with a productivity of 0.92 g/Lh. Analysis of raw material cost component for pullulan production suggested that sole utilization of agri-residues may lead to development of cost effective process for pullulan production.
authors.affiliation: CSIR-Institute of Microbial Technology (IMTECH), Council of Scientific and Industrial Research (CSIR), Sector - 39A, Chandigarh 160036, India.
authors.forename: Ananya
authors.initials: A
authors.lastname: Mehta
authors.affiliation: CSIR-Institute of Microbial Technology (IMTECH), Council of Scientific and Industrial Research (CSIR), Sector - 39A, Chandigarh 160036, India.
authors.forename: G S
authors.initials: GS
authors.lastname: Prasad
authors.affiliation: CSIR-Institute of Microbial Technology (IMTECH), Council of Scientific and Industrial Research (CSIR), Sector - 39A, Chandigarh 160036, India. Electronic address: anirban@imtech.res.in.
authors.forename: Anirban Roy
authors.initials: AR
authors.lastname: Choudhury
mesh_terms: D005285:Fermentation; D005936:Glucans / Q000096:biosynthesis*; D014866:Waste Products
chemical_list: D005936:Glucans; D014866:Waste Products; C009109:pullulan
keywords: Agri-industrial waste; Pullulan; Response surface methodology