title: A modified dinucleotide for site-specific RNA-labelling by transcription priming and click chemistry.
abstract: An improved strategy for RNA labelling using an alkyne-carrying dinucleotide is reported. This involves near-quantitative priming by phage RNA-polymerases followed by conjugation of different labels using click chemistry. Moreover, these transcripts bear a ligation compatible 5'-end, and thus through ligation the terminal label can be transformed to an internal one.
authors.affiliation: Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg 69120, Germany. jaeschke@uni-hd.de.
authors.forename: Ayan
authors.initials: A
authors.lastname: Samanta
authors.forename: André
authors.initials: A
authors.lastname: Krause
authors.forename: Andres
authors.initials: A
authors.lastname: Jäschke
mesh_terms: D002384:Catalysis; D057930:Click Chemistry; D003300:Copper / Q000737:chemistry; D061565:Cycloaddition Reaction; D009711:Nucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D014158:Transcription, Genetic
chemical_list: D009711:Nucleotides; D012313:RNA; D003300:Copper