title: Decreased CD127 expression on CD4+ T-cells and elevated frequencies of CD4+CD25+CD127- T-cells in children with long-lasting type 1 diabetes.
abstract: Pathobiology of type 1 diabetes (T1D) is predominantly associated with T-cell-related actions. Homeostasis of majority of T-cells is critically dependent on signals mediated by CD127 (interleukin-7 receptor, IL-7R). In contrast, regulatory T-cells express very little CD127 and thereby may be delineated by CD4+CD25+CD127- phenotype. Here we aimed to analyze CD127 expression on CD4+ and CD8+ T-cells and enumerate CD4+CD25+CD127- T-cells in long-lasting T1D. T-cells were analyzed by flow cytometry and immunologic data were correlated with vascular, metabolic, and inflammatory parameters. We demonstrated significantly decreased CD127 levels on CD4+, but not CD8+, T cells in T1D pediatric patients. Interestingly, frequencies of CD4+CD25+CD127- T-cells were significantly enhanced in T1D children and correlated well with frequencies of CD34+CD144+ endothelial progenitor cells and CD4+CD25- T-cells. Levels of CD127 on both CD4+ and CD8+ T-cells in T1D patients were not correlated to each other or HbA1C. Interestingly, however, CD127 levels on CD4+ T-cells were significantly correlated to frequencies of CD4+CD25+CD127- T-cells, whereas CD127 levels on CD8+ T-cells were significantly correlated to concentrations of VEGF and triglycerides. Our data indicate that CD127 expression is differentially modulated on CD4+ and CD8+ T-cells in the course of T1D. Moreover, we demonstrated that, in contrast to recent-onset T1D, long-lasting T1D is associated with enhancement of T-cells with regulatory phenotype.
authors.affiliation: Department of Regenerative Medicine and Immune Regulation, Medical University of Bialystok, 15-269 Bialystok, Poland ; Department of Allergology and Internal Medicine, University of Bialystok, 15-276 Bialystok, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Moniuszko
authors.affiliation: Department of Pediatrics, Endocrinology, Diabetology with Cardiology Division, Medical University of Bialystok, 15-274 Bialystok, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Glowinska-Olszewska
authors.affiliation: Department of Hematological Diagnostics, Medical University of Bialystok, 15-274 Bialystok, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Rusak
authors.affiliation: Department of Allergology and Internal Medicine, University of Bialystok, 15-276 Bialystok, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Jeznach
authors.affiliation: Department of Immunology, Medical University of Bialystok, 15-276 Bialystok, Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Grubczak
authors.affiliation: Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, 15-276 Bialystok, Poland.
authors.forename: Danuta
authors.initials: D
authors.lastname: Lipinska
authors.affiliation: Department of Statistics and Medical Informatics, Medical University of Bialystok, 15-295 Bialystok, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Milewski
authors.affiliation: Department of Statistics and Medical Informatics, Medical University of Bialystok, 15-295 Bialystok, Poland.
authors.forename: Anna Justyna
authors.initials: AJ
authors.lastname: Milewska
authors.affiliation: Department of Hematological Diagnostics, Medical University of Bialystok, 15-274 Bialystok, Poland.
authors.forename: Milena
authors.initials: M
authors.lastname: Dabrowska
authors.affiliation: Department of Immunology, Medical University of Bialystok, 15-276 Bialystok, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Jablonska
authors.affiliation: Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, 15-276 Bialystok, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Kretowski
authors.affiliation: Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, 15-276 Bialystok, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Gorska
authors.affiliation: Department of Allergology and Internal Medicine, University of Bialystok, 15-276 Bialystok, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Bodzenta-Lukaszyk
authors.affiliation: Department of Pediatrics, Endocrinology, Diabetology with Cardiology Division, Medical University of Bialystok, 15-274 Bialystok, Poland.
authors.forename: Artur
authors.initials: A
authors.lastname: Bossowski
mesh_terms: D000293:Adolescent; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000175:diagnosis / Q000276:immunology* / Q000378:metabolism*; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D053699:Interleukin-7 Receptor alpha Subunit / Q000378:metabolism*; D018655:Lymphocyte Count; D008297:Male; D010641:Phenotype; D012307:Risk Factors
chemical_list: D053645:Interleukin-2 Receptor alpha Subunit; D053699:Interleukin-7 Receptor alpha Subunit