title: Distribution and accumulation of Cy5.5-labeled thermally cross-linked superparamagnetic iron oxide nanoparticles in the tissues of ICR mice.
abstract: Free Cy5.5 dye and Cy5.5-labeled thermally cross-linked superparamagnetic iron oxide nanoparticles (TCL-SPION) have been routinely used for in vivo optical imaging. However, there is little information about the distribution and accumulation of free Cy5.5 dye and Cy5.5-labeled TCL-SPION in the tissues of mice. Free Cy5.5 dye (0.1 mg/kg body weight) and Cy5.5-labeled TCL-SPION (15 mg/kg body weight) were intravenously injected into the tail vein of ICR mice. The biodistribution and accumulation of the TCL-SPION and Cy5.5 were observed by ex vivo optical imaging and fluorescence signal generation at various time points over 28 days. Cy5.5 dye fluorescence in various organs was rapidly eliminated from 0.5 to 24 h post-injection. Fluorescence intensity of Cy5.5 dye in the liver, lung, kidney, and stomach was fairly strong at the early time points within 1 day post-injection. Cy5.5-labeled TCL-SPION had the highest fluorescence density in the lung at 0.5 h post-injection and decreased rapidly over time. Fluorescence density in liver and spleen was maintained over 28 days. These results suggest that TCL-SPION can be useful as a carrier of therapeutic reagents to treat diseases by persisting for long periods of time in the body.
authors.affiliation: College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea.
authors.forename: Jin Joo
authors.initials: JJ
authors.lastname: Hue
authors.forename: Hu-Jang
authors.initials: HJ
authors.lastname: Lee
authors.forename: Sangyong
authors.initials: S
authors.lastname: Jon
authors.forename: Sang Yoon
authors.initials: SY
authors.lastname: Nam
authors.forename: Young Won
authors.initials: YW
authors.lastname: Yun
authors.forename: Jong-Soo
authors.initials: JS
authors.lastname: Kim
authors.forename: Beom Jun
authors.initials: BJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000494:pharmacology*; D005290:Ferric Compounds / Q000494:pharmacology*; D005456:Fluorescent Dyes / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D053758:Nanoparticles / Q000378:metabolism*; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D005290:Ferric Compounds; D005456:Fluorescent Dyes; C000499:ferric oxide
keywords: Cy5.5 dye; accumulation; biodistribution; thermally cross-linked superparamagnetic iron oxide nanoparticles; toxicity