title: [Simultaneous determination of 6 neonicotinoid residues in soil using DLLME-HPLC and UV].
abstract: A simple, cheap and rugged method was developed for simultaneous deter mination of 6 neonicotinoid residues in soil, including imidacloprid, acetamiprid, thiamethoxam, thiacloprid, clothianidin and nitenpyram. The soil sample was produced by dispersive liquid-liquid micro-extraction (DLLME) after extracted by the mixed solution of acetonitrile and CH2Cl2 (2:1, phi). The analytes were separated by HPLC with Alltima C18 column (4.6 mm x 250 mm, 5 microm) and detected by PDA at 260 nm. External standard method was used for quantification. The results showed that good linearity was obtained with correlation coefficients between 0.9982 and 0.9999 in the range of 0.5-200 microg x L(-1). The limits of detection (LODs) were in the range between 0.0005 and 0.003 microg x mL(-1) (S/N = 3). The method was validated with five soil samples spiked at three fortification levels (0.05, 0.1, 1.0 mg x kg(-1)) and recoveries were in the range of 55.3%-95.6% with RSD of 1.4%-7.0%. The effect of clean-up was evaluated by UV spectra and demonstrated that the method established is effective. In conclusion, this method is competent for the simultaneous analysis of 6 neonicotinoid residues in soil.
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-environment and Climate Change of Ministy of Agriculture, Beijing 100081, China. baolisun_6@126.com
authors.forename: Bao-li
authors.initials: BL
authors.lastname: Sun
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-environment and Climate Change of Ministy of Agriculture, Beijing 100081, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Shan
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-environment and Climate Change of Ministy of Agriculture, Beijing 100081, China.
authors.forename: Yan-hua
authors.initials: YH
authors.lastname: Li
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-environment and Climate Change of Ministy of Agriculture, Beijing 100081, China.
authors.forename: Ya-ling
authors.initials: YL
authors.lastname: Zeng
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-environment and Climate Change of Ministy of Agriculture, Beijing 100081, China.
authors.forename: Xiu-li
authors.initials: XL
authors.lastname: Shen
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-environment and Climate Change of Ministy of Agriculture, Beijing 100081, China.
authors.forename: Cheng-feng
authors.initials: CF
authors.lastname: Tong
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006146:Guanidines; D007093:Imidazoles; D057230:Limit of Detection; D000073943:Neonicotinoids; D009574:Nitro Compounds; D010078:Oxazines; D010573:Pesticide Residues / Q000032:analysis*; D011725:Pyridines; D012987:Soil / Q000737:chemistry*; D000077922:Thiamethoxam; D013843:Thiazines; D013844:Thiazoles
chemical_list: D006146:Guanidines; D007093:Imidazoles; D000073943:Neonicotinoids; D009574:Nitro Compounds; D010078:Oxazines; D010573:Pesticide Residues; D011725:Pyridines; D012987:Soil; D013843:Thiazines; D013844:Thiazoles; C480342:clothianidin; C464843:nitenpyram; C082359:imidacloprid; C464485:acetamiprid; D000077922:Thiamethoxam; C417209:thiacloprid