title: Ubiquitin-specific peptidase 5, a target molecule of vialinin A, is a key molecule of TNF-α production in RBL-2H3 cells.
abstract: Tumor necrosis factor alpha (TNF-α), a central mediator of the inflammatory response, is released from basophilic cells and other cells in response to a variety of proinflammatory stimuli. Vialinin A is a potent inhibitor of TNF-α production and is released from RBL-2H3 cells. Ubiquitin-specific peptidase 5 (USP5), a deubiquitinating enzyme, was identified as a target molecule of vialinin A and its enzymatic activity was inhibited by vialinin A. Here we report production of TNF-α is decreased in USP5 siRNA-knockdown RBL-2H3 cells, compared with control cells. The finding of the present study strongly suggests that USP5 is one of the essential molecules for the production of TNF-α in RBL-2H3.
authors.affiliation: Department of Nutritional Science, Faculty of Applied Bio-science, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Yasukiyo
authors.initials: Y
authors.lastname: Yoshioka
authors.affiliation: Department of Nutritional Science, Faculty of Applied Bio-science, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Yue Qi
authors.initials: YQ
authors.lastname: Ye
authors.affiliation: Department of Bioscience, Faculty of Applied Bio-science, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Okada
authors.affiliation: Department of Fermentation Science, Faculty of Applied Bio-science, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Kayoko
authors.initials: K
authors.lastname: Taniguchi
authors.affiliation: RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama, Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Yoshida
authors.affiliation: Department of Nutritional Science, Faculty of Applied Bio-science, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Sugaya
authors.affiliation: Department of Nutritional Science, Faculty of Applied Bio-science, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Onose
authors.affiliation: RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Koshino
authors.affiliation: RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama, Japan.
authors.forename: Shunya
authors.initials: S
authors.lastname: Takahashi
authors.affiliation: Department of Fermentation Science, Faculty of Applied Bio-science, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Arata
authors.initials: A
authors.lastname: Yajima
authors.affiliation: Department of Bioscience, Faculty of Applied Bio-science, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Yajima
authors.affiliation: Department of Nutritional Science, Faculty of Applied Bio-science, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Abe
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D010450:Endopeptidases / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D034741:RNA, Small Interfering; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013730:Terphenyl Compounds / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; D013730:Terphenyl Compounds; D014409:Tumor Necrosis Factor-alpha; C508745:vialinin A; D015847:Interleukin-4; D010450:Endopeptidases; C091939:ubiquitin isopeptidase