title: Designing nanotopographical density of extracellular matrix for controlled morphology and function of human mesenchymal stem cells.
abstract: Inspired by ultrastructural analysis of ex vivo human tissues as well as the physiological importance of structural density, we fabricated nanogrooves with 1:1, 1:3, and 1:5 spacing ratio (width:spacing, width = 550 nm). In response to the nanotopographical density, the adhesion, migration, and differentiation of human mesenchymal stem cells (hMSCs) were sensitively controlled, but the proliferation showed no significant difference. In particular, the osteo- or neurogenesis of hMSCs were enhanced at the 1:3 spacing ratio rather than 1:1 or 1:5 spacing ratio, implying an existence of potentially optimized nanotopographical density for stem cell function. Furthermore, such cellular behaviors were positively correlated with several cell morphological indexes as well as the expression of integrin β1 or N-cadherin. Our findings propose that nanotopographical density may be a key parameter for the design and manipulation of functional scaffolds for stem cell-based tissue engineering and regenerative medicine.
authors.affiliation: 1] Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul, 151-742, Republic of Korea [2].
authors.forename: Jangho
authors.initials: J
authors.lastname: Kim
authors.affiliation: 1] Division of WCU Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, 151-742, Republic of Korea [2].
authors.forename: Hong Nam
authors.initials: HN
authors.lastname: Kim
authors.affiliation: Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul, 151-742, Republic of Korea.
authors.forename: Ki-Taek
authors.initials: KT
authors.lastname: Lim
authors.affiliation: Department of Otolaryngology, Ajou University School of Medicine, Suwon, 443-721, Republic of Korea.
authors.forename: Yeonju
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul, 151-742, Republic of Korea.
authors.forename: Hoon
authors.initials: H
authors.lastname: Seonwoo
authors.affiliation: Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul, 151-742, Republic of Korea.
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Otolaryngology, Ajou University School of Medicine, Suwon, 443-721, Republic of Korea.
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Lim
authors.affiliation: Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.
authors.forename: Deok-Ho
authors.initials: DH
authors.lastname: Kim
authors.affiliation: 1] Division of WCU Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, 151-742, Republic of Korea [2].
authors.forename: Kahp-Yang
authors.initials: KY
authors.lastname: Suh
authors.affiliation: Tooth Bioengineering National Research Lab, Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Pill-Hoon
authors.initials: PH
authors.lastname: Choung
authors.affiliation: Department of Otolaryngology, Ajou University School of Medicine, Suwon, 443-721, Republic of Korea.
authors.forename: Yun-Hoon
authors.initials: YH
authors.lastname: Choung
authors.affiliation: 1] Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul, 151-742, Republic of Korea [2] Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Chung
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D001672:Biocompatible Materials / Q000138:chemical synthesis; D015820:Cadherins / Q000096:biosynthesis; D002448:Cell Adhesion; D002452:Cell Count; D018929:Cell Culture Techniques; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000502:physiology*; D006801:Humans; D019012:Integrin beta1 / Q000096:biosynthesis; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D055495:Neurogenesis; D010012:Osteogenesis; D013499:Surface Properties; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D015820:Cadherins; D019012:Integrin beta1