title: Cytotoxic, mutagenic and genotoxic evaluation of crude extracts and fractions from Piper jericoense with trypanocidal action.
abstract: The current Chagas disease treatment is based on two drugs, nifurtimox and benznidazole, which is considered unsatisfactory, not only because of the narrow therapeutic range but also because of the associated toxicity. Natural products are considered an important source of biologically active compounds against various infectious organisms. Numerous Piper species are used in traditional medicine to treat parasitic diseases. In this paper, we study the activity of extracts and fractions obtained from Piper jericoense plant against epimastigote, trypomastigote and amastigote forms of Trypanosoma cruzi. In addition, we evaluated the cytotoxic, mutagenic and genotoxic activities of the F4 fraction obtained from one of the more promising extracts. We obtained four extracts, one of which presented low toxicity and high trypanocidal activity. This extract was separated into eight fractions, and the F4 fraction presented better results than the other extracts and had a higher selectivity index than the reference drug, benznidazole. This fraction was not cytotoxic, mutagenic or genotoxic.
authors.affiliation: Grupo Biología y Control de Enfermedades Infecciosas-BCEI, Universidad de Antioquia UdeA, Calle 70 No. 52-21, Medellín, Colombia.
authors.forename: A L
authors.initials: AL
authors.lastname: Hamedt
authors.affiliation: Grupo de Biología de Sistemas, Universidad Pontificia Bolivariana, Calle 78b 72a-109, Medellín, Colombia.
authors.forename: I C
authors.initials: IC
authors.lastname: Ortiz
authors.affiliation: Grupo Biología y Control de Enfermedades Infecciosas-BCEI, Universidad de Antioquia UdeA, Calle 70 No. 52-21, Medellín, Colombia.
authors.forename: P A
authors.initials: PA
authors.lastname: García-Huertas
authors.affiliation: Laboratorio Química de Productos Naturales, Universidad de Antioquia, Calle 70 No. 52-21, Medellin, Colombia.
authors.forename: J
authors.initials: J
authors.lastname: Sáenz
authors.affiliation: Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Departamento de Biofisica e Biometria, Rio de Janeiro 87-20551-030, RJ, Brazil.
authors.forename: A Caldeira
authors.initials: AC
authors.lastname: de Araujo
authors.affiliation: Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Departamento de Biofisica e Biometria, Rio de Janeiro 87-20551-030, RJ, Brazil.
authors.forename: J C P
authors.initials: JC
authors.lastname: De Mattos
authors.affiliation: Universidad de Antioquia, Calle 67 53-108, Medellín, Colombia.
authors.forename: M A
authors.initials: MA
authors.lastname: Rodríguez-Gazquez
authors.affiliation: Grupo Biología y Control de Enfermedades Infecciosas-BCEI, Universidad de Antioquia UdeA, Calle 70 No. 52-21, Medellín, Colombia. Electronic address: otriana@gmail.com.
authors.forename: O
authors.initials: O
authors.lastname: Triana-Chávez
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005591:Chemical Fractionation; D003470:Culture Media; D006801:Humans; D008018:Life Cycle Stages / Q000187:drug effects*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D009152:Mutagenicity Tests; D009547:Nifurtimox / Q000494:pharmacology; D009593:Nitroimidazoles / Q000494:pharmacology; D021261:Parasitic Sensitivity Tests; D031701:Piper / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D014344:Trypanocidal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000254:growth & development
chemical_list: D003470:Culture Media; D009593:Nitroimidazoles; D010936:Plant Extracts; D014344:Trypanocidal Agents; D009547:Nifurtimox; C009999:benzonidazole
keywords: Antiprotozoal; Chagas; Chemotherapy; Cytotoxicity; Genotoxicity; Mutagenesis