title: Mining and assessment of catabolic pathways in the metagenome of a common effluent treatment plant to induce the degradative capacity of biomass.
abstract: Metagenome analysis was used to understand the microbial community in activated sludge treating industrial wastewaters at a Common Effluent Treatment Plant (CETP) in South India. The taxonomic profile mapped onto National Center for Biotechnology Information (NCBI) taxonomy using MEtaGenome ANalyzer (MEGAN), demonstrated that the most abundant domain belonged to prokaryotes, dominated by bacteria. Bacteria representing nine phyla were identified from the sequence data including representatives from two new phyla, Synergistetes and Elusimicrobia. Functional analysis of the metagenome, with specific reference to the metabolism of aromatic compounds, revealed the dominance of genes of the central meta-cleavage pathway. This information was used to improve the degradative efficiency in the wastewater treatment plant. A pilot scale plant was set up with 200L of activated sludge using salicylate induced sludge and results demonstrated 52% removal in chemical oxygen demand (COD) against non-induced biomass.
authors.affiliation: Environmental Genomics Division, National Environmental Engineering Research Institute (CSIR-NEERI), Nagpur 440020 (MH), India.
authors.forename: Ravi P
authors.initials: RP
authors.lastname: More
authors.affiliation: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551, Singapore.
authors.forename: Suparna
authors.initials: S
authors.lastname: Mitra
authors.affiliation: Environmental Genomics Division, National Environmental Engineering Research Institute (CSIR-NEERI), Nagpur 440020 (MH), India.
authors.forename: Sajan C
authors.initials: SC
authors.lastname: Raju
authors.affiliation: Environmental Genomics Division, National Environmental Engineering Research Institute (CSIR-NEERI), Nagpur 440020 (MH), India. Electronic address: a_kapley@neeri.res.in.
authors.forename: Atya
authors.initials: A
authors.lastname: Kapley
authors.affiliation: Environmental Genomics Division, National Environmental Engineering Research Institute (CSIR-NEERI), Nagpur 440020 (MH), India.
authors.forename: Hemant J
authors.initials: HJ
authors.lastname: Purohit
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics*; D001673:Biodegradation, Environmental; D044822:Biodiversity; D018533:Biomass; D057225:Data Mining; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D054892:Metagenome; D010100:Oxygen / Q000378:metabolism; D010802:Phylogeny; D010865:Pilot Projects; D012722:Sewage / Q000382:microbiology; D013045:Species Specificity; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000382:microbiology; D018508:Water Purification / Q000379:methods*
chemical_list: D010100:Oxygen; D012722:Sewage; D062065:Wastewater
keywords: Activated sludge; CETP; Catabolic genes; Metagenome; Microbial diversity