title: Evaluation of triple stage mass spectrometry as a robust and accurate diagnostic tool for determination of free cordycepin in designer egg.
abstract: Direct determination of free cordycepin in designer egg using a highly selective mass spectrometric (MS) technique aided by a rapid and efficient dilute-and-shoot workflow would enhance their application as diagnostic tools in food fraud control. Here, triple stage mass spectrometry (MS(3)) demonstrated excellent analyte selectivity capability even when incomplete chromatographic separation was performed. Method validation was performed at six concentration levels of 100, 200, 400, 800, 1200 and 1600ngg(-1). Spiking experiments were examined at three concentration levels of 200, 400, and 1200ngg(-1) in individual egg white and egg yolk, measured over 2days. MS(3) enabled ion chromatograms with zero-background interference to be made in egg extracts. MS(3) eliminated severe over recovery (p<0.05) observed in all fortified samples, a challenge that MRM-transition could not address in a single step. Matrix-matched calibrants were needed to compensate for over recovery observed under MRM-transition mode.
authors.affiliation: Food Science & Technology Programme, c/o Department of Chemistry, National University of Singapore, S14-Level 5, Science Drive 2, Singapore 117542, Singapore.
authors.forename: Yi Hsin
authors.initials: YH
authors.lastname: Chen
authors.affiliation: Food Safety Laboratory, Applied Sciences Group, Health Sciences Authority, 11 Outram Road, Singapore 169078, Singapore. Electronic address: Jeff_lim@hsa.gov.sg.
authors.forename: Chee Wei
authors.initials: CW
authors.lastname: Lim
authors.affiliation: Food Safety Laboratory, Applied Sciences Group, Health Sciences Authority, 11 Outram Road, Singapore 169078, Singapore.
authors.forename: Sheot Harn
authors.initials: SH
authors.lastname: Chan
mesh_terms: D000818:Animals; D002645:Chickens; D003839:Deoxyadenosines / Q000032:analysis* / Q000302:isolation & purification; D004531:Eggs / Q000032:analysis*; D055951:Functional Food / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D003839:Deoxyadenosines; C058120:cordycepin
keywords: Diagnostic; Free cordycepin; MS/MS/MS (MS(3)); Quantitation; Selectivity