title: Fluorescence Investigation of Interactions Between Novel Benzanthrone Dyes and Lysozyme Amyloid Fibrils.
abstract: A series of novel fluorescent benzanthrone dyes have been tested for their ability to identify and characterize fibrillar aggregates of lysozyme prepared by protein denaturation in concentrated ethanol solution (F(eth)) or acidic buffer (F(ac)). Quantitative parameters of the dye association with native and fibrillar protein have been derived from the results of fluorimetric titration. The binding characteristics proved to be different for F(eth)- and F(ac)-bound benzanthrones, highlighting the dye sensitivity to the distinctions in fibril morphology. By comparing the dye preference to fibrillar protein aggregates, AM2, A8 and A6 were selected as the most prospective amyloid tracers. Based on the analysis of red edge excitation shifts and fluorescence lifetimes of the amyloid-bound dyes it was assumed that surface grooves or dry "steric zipper" interface are potential fibril binding sites for the novel fluorophores.
authors.affiliation: Department of Biological and Medical Physics, V.N. Karazin Kharkiv National University, 4 Svobody Sq, 61022, Kharkiv, Ukraine. katenka_vus@mail.ru.
authors.forename: Kateryna
authors.initials: K
authors.lastname: Vus
authors.affiliation: Department of Biological and Medical Physics, V.N. Karazin Kharkiv National University, 4 Svobody Sq, 61022, Kharkiv, Ukraine.
authors.forename: Valeriya
authors.initials: V
authors.lastname: Trusova
authors.affiliation: Department of Biological and Medical Physics, V.N. Karazin Kharkiv National University, 4 Svobody Sq, 61022, Kharkiv, Ukraine.
authors.forename: Galyna
authors.initials: G
authors.lastname: Gorbenko
authors.affiliation: Department of Biomedical Engineering and Computational Science, School of Science and Technology, Aalto University, Otakaari 3, FI-00076, Espoo, Finland.
authors.forename: Rohit
authors.initials: R
authors.lastname: Sood
authors.affiliation: Department of Chemistry and Geography, Faculty of Natural Sciences and Mathematics, Daugavpils University, Vienibas 13, LV5401, Daugavpils, Latvia.
authors.forename: Elena
authors.initials: E
authors.lastname: Kirilova
authors.affiliation: Department of Chemistry and Geography, Faculty of Natural Sciences and Mathematics, Daugavpils University, Vienibas 13, LV5401, Daugavpils, Latvia.
authors.forename: Georgiy
authors.initials: G
authors.lastname: Kirilov
authors.affiliation: Department of Chemistry and Geography, Faculty of Natural Sciences and Mathematics, Daugavpils University, Vienibas 13, LV5401, Daugavpils, Latvia.
authors.forename: Inta
authors.initials: I
authors.lastname: Kalnina
authors.affiliation: Department of Biomedical Engineering and Computational Science, School of Science and Technology, Aalto University, Otakaari 3, FI-00076, Espoo, Finland.
authors.forename: Paavo
authors.initials: P
authors.lastname: Kinnunen
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D001551:Benz(a)Anthracenes / Q000737:chemistry*; D052160:Benzothiazoles; D004396:Coloring Agents / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D009113:Muramidase / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013844:Thiazoles / Q000737:chemistry
chemical_list: D000682:Amyloid; D001551:Benz(a)Anthracenes; D052160:Benzothiazoles; D004396:Coloring Agents; D013844:Thiazoles; C009462:thioflavin T; D009113:Muramidase; C012768:benzanthrone
keywords: Amyloid marker; Benzanthrone dyes; Fibrillar lysozyme; Polarity