title: LASIK following small incision lenticule extraction (SMILE) lenticule re-implantation: a feasibility study of a novel method for treatment of presbyopia.
abstract: Presbyopia remains a major visual impairment for patients, who have previously undergone laser refractive correction and enjoyed unaided distance vision prior to the onset of presbyopia. Corneal stromal volume restoration through small incision lenticule extraction (SMILE) lenticule re-implantation presents an opportunity for restoring the patients' non-dominant eye to previous low myopia to achieve a monovision. In this study, we investigated the feasibility of performing LASIK after lenticule re-implantation as a method to create presbyopic monovision. A -6.00D SMILE correction was performed in 9 rabbit eyes. The lenticules were cryopreserved for 14 days and re-implanted. Five weeks later, 3 of these eyes underwent LASIK for -5.00D correction (RL group); 3 underwent LASIK flap creation, which was not lifted (RN); and no further procedures were performed on the remaining 3 eyes. These groups were compared with 3 eyes that underwent standard LASIK for a -5.00D correction (LO); 3 that underwent creation of non-lifted flap (LN); and 3 non-operated eyes. Rabbits were euthanized 1 day post-surgery. Tissue responses were analyzed by immunohistochemistry, slit lamp and in vivo confocal microscopy (IVCM). Intrastromal irregularities and elevated reflectivity levels of the excimer-ablated plane were observed on slit lamp and IVCM, respectively in the RL group. The results were comparable (P = 0.310) to IVCM findings in the LO group. RL and LO groups showed similar fibronectin expression levels, number of CD11b-positive cells (P = 0.304) and apoptotic cells (P = 0.198). There was no difference between the RN and LN groups in reflectivity levels (P = 0.627), fibronectin expression levels, CD11b-positive cells (P = 0.135) and apoptotic cells (P = 0.128). LASIK can be performed following lenticule re-implantation to create presbyopic monovision. The tissue responses elicited after performing LASIK on corneas that have undergone SMILE and subsequent lenticule re-implantation are similar to primary procedure.
authors.affiliation: Faculty of Medicine, University of New South Wales, Sydney, New South Wales, Australia ; Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore, Singapore ; Singapore National Eye Centre, Singapore, Singapore.
authors.forename: Chris H L
authors.initials: CH
authors.lastname: Lim
authors.affiliation: Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore, Singapore.
authors.forename: Andri K
authors.initials: AK
authors.lastname: Riau
authors.affiliation: Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore, Singapore.
authors.forename: Nyein C
authors.initials: NC
authors.lastname: Lwin
authors.affiliation: Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore, Singapore ; Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore ; SRP Neuroscience and Behavioral Disorders, Duke-NUS Graduate Medical School, Singapore, Singapore.
authors.forename: Shyam S
authors.initials: SS
authors.lastname: Chaurasia
authors.affiliation: Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore, Singapore ; Singapore National Eye Centre, Singapore, Singapore ; Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
authors.forename: Donald T
authors.initials: DT
authors.lastname: Tan
authors.affiliation: Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore, Singapore ; Singapore National Eye Centre, Singapore, Singapore ; Department of Clinical Sciences, Duke-NUS Graduate Medical School, Singapore, Singapore.
authors.forename: Jodhbir S
authors.initials: JS
authors.lastname: Mehta
mesh_terms: D000818:Animals; D006801:Humans; D020731:Keratomileusis, Laser In Situ / Q000379:methods*; D011305:Presbyopia / Q000503:physiopathology* / Q000601:surgery*; D011817:Rabbits; D020127:Recovery of Function; D014785:Vision, Ocular