title: Effect of TiO2 nanofibres on selected bronchoalveolar parameters in acute and subacute phase--experimental study.
abstract: Titanium dioxide nanofibres (TiO2) were intratracheally instilled in dose of 4 mg/0.2 mL saline solution per animal (Wistar rats). After 48 hours and 14 days the animals were exsanguinated (under i.p. thiopental narcosis), bronchoalveolar lavage (BAL) was perfomed and cells from BAL fluid were isolated. Following inflammatory, cytotoxic and oxidative stress BAL parameters were examined: differential cell count (% of alveolar macrophages (AM), polymorphonuclears and lymphocytes); the viability and phagocytic activity of AM; the proportion of immature cells; the proportion of multinucleated cells; count of AM/mL lavage; count of BAL cells/mL lavage; the level of ascorbic acid and activity of superoxide dismutase, both in tissue homogenate and in bronchoalveolar lavage fluid. The majority of examined BAL parameters in the acute and subacute phase in our study suggest serious inflammatory and cytotoxic processes in lung after exposure to TiO2.
authors.affiliation: Slovak Medical University, Faculty of Public Health, Laboratory of Respiratory Toxicology, Bratislava, Slovakia. marta.hurbankova@szu.sk
authors.forename: Marta
authors.initials: M
authors.lastname: Hurbánková
authors.affiliation: Slovak Medical University, Faculty of Public Health, Laboratory of Respiratory Toxicology, Bratislava, Slovakia.
authors.forename: Silvia
authors.initials: S
authors.lastname: Cerná
authors.affiliation: Slovak Medical University, Faculty of Public Health, Laboratory of Respiratory Toxicology, Bratislava, Slovakia.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Kováciková
authors.affiliation: Slovak Medical University, Faculty of Public Health, Laboratory of Respiratory Toxicology, Bratislava, Slovakia.
authors.forename: Sonia
authors.initials: S
authors.lastname: Wimmerová
authors.affiliation: Slovak Medical University, Faculty of Public Health, Laboratory of Respiratory Toxicology, Bratislava, Slovakia.
authors.forename: Dominika
authors.initials: D
authors.lastname: Hrasková
authors.affiliation: Medical-Preventive Limited Liability Company, Hnusta, Slovakia.
authors.forename: Jana
authors.initials: J
authors.lastname: Marcisiaková
authors.affiliation: Slovak Medical University, Faculty of Public Health, Laboratory of Respiratory Toxicology, Bratislava, Slovakia.
authors.forename: Stefánia
authors.initials: S
authors.lastname: Moricová
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D018836:Inflammation Mediators / Q000276:immunology; D007962:Leukocytes / Q000276:immunology; D016676:Macrophages, Alveolar / Q000276:immunology; D057139:Nanofibers / Q000008:administration & dosage / Q000009:adverse effects*; D018384:Oxidative Stress / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D014025:Titanium / Q000008:administration & dosage / Q000009:adverse effects* / Q000276:immunology
chemical_list: D018836:Inflammation Mediators; C009495:titanium dioxide; D014025:Titanium