title: In vitro effect of genistein on DNA damage in leukocytes from mucopolysaccharidosis IVA patients.
abstract: Mucopolysaccharidosis IVA is a lysosomal storage disorder leading to an increase in glycosaminoglycans storage. Genistein is an isoflavone capable to inhibit glycosaminoglycans production. The objective of this study was to analyze the in vitro effect of different concentrations of genistein on DNA injury in mucopolysaccharidosis IVA patients. The lower concentration tested (10 μM) showed a significant increase on DNA injury in vitro, although higher concentrations (30 μM and 50 μM) showed higher DNA damage.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga, 2752, 1º andar, CEP: 90610-000 Porto Alegre, RS, Brazil; Serviço de Genética Médica, Hospital de Clínicas de Porto Alegre (HCPA), Ramiro Barcelos 2350, Porto Alegre, RS 90035-903, Brazil. Electronic address: gnegretto@gmail.com.
authors.forename: G W
authors.initials: GW
authors.lastname: Negretto
authors.affiliation: Serviço de Genética Médica, Hospital de Clínicas de Porto Alegre (HCPA), Ramiro Barcelos 2350, Porto Alegre, RS 90035-903, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Deon
authors.affiliation: Serviço de Genética Médica, Hospital de Clínicas de Porto Alegre (HCPA), Ramiro Barcelos 2350, Porto Alegre, RS 90035-903, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Burin
authors.affiliation: Serviço de Genética Médica, Hospital de Clínicas de Porto Alegre (HCPA), Ramiro Barcelos 2350, Porto Alegre, RS 90035-903, Brazil.
authors.forename: G B
authors.initials: GB
authors.lastname: Biancini
authors.affiliation: Serviço de Genética Médica, Hospital de Clínicas de Porto Alegre (HCPA), Ramiro Barcelos 2350, Porto Alegre, RS 90035-903, Brazil.
authors.forename: G
authors.initials: G
authors.lastname: Ribas
authors.affiliation: Faculdade de Farmácia, UFRGS, Laboratório de Toxicologia, Endereço: Avenida Ipiranga, 2752, sala 605, CEP: 90610-000, Brazil.
authors.forename: S C
authors.initials: SC
authors.lastname: Garcia
authors.affiliation: Faculdade de Farmácia, UFRGS, Laboratório de Toxicologia, Endereço: Avenida Ipiranga, 2752, sala 605, CEP: 90610-000, Brazil.
authors.forename: G
authors.initials: G
authors.lastname: Goethel
authors.affiliation: Faculdade de Farmácia, UFRGS, Laboratório de Toxicologia, Endereço: Avenida Ipiranga, 2752, sala 605, CEP: 90610-000, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Fracasso
authors.affiliation: Serviço de Genética Médica, Hospital de Clínicas de Porto Alegre (HCPA), Ramiro Barcelos 2350, Porto Alegre, RS 90035-903, Brazil.
authors.forename: L
authors.initials: L
authors.lastname: Giugliani
authors.affiliation: Serviço de Genética Médica, Hospital de Clínicas de Porto Alegre (HCPA), Ramiro Barcelos 2350, Porto Alegre, RS 90035-903, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Giugliani
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga, 2752, 1º andar, CEP: 90610-000 Porto Alegre, RS, Brazil; Serviço de Genética Médica, Hospital de Clínicas de Porto Alegre (HCPA), Ramiro Barcelos 2350, Porto Alegre, RS 90035-903, Brazil. Electronic address: crvargas@hcpa.ufrgs.br.
authors.forename: C R
authors.initials: CR
authors.lastname: Vargas
mesh_terms: D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D002648:Child; D020552:Comet Assay; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000473:pathology; D008297:Male; D009085:Mucopolysaccharidosis IV / Q000473:pathology*
chemical_list: D019833:Genistein
keywords: Comet assay; DNA damage; Genistein; Genotoxicity; Mucopolysaccharidosis IVA