title: Generation of leukemia inhibitory factor-dependent induced pluripotent stem cells from the Massachusetts General Hospital miniature pig.
abstract: The generation and application of porcine induced pluripotent stem cells (iPSCs) may enable the testing for safety and efficacy of therapy in the field of human regenerative medicine. Here, the generation of iPSCs from the Massachusetts General Hospital miniature pig (MGH minipig) established for organ transplantation studies is reported. Fibroblasts were isolated from the skin of the ear of a 10-day-old MGH minipig and transduced with a cocktail of six human factors: POU5F1, NANOG, SOX2, C-MYC, KLF4, and LIN28. Two distinct types of iPSCs were generated that were positive for alkaline phosphatase activity, as well as the classical pluripotency markers: Oct4, Nanog, Sox2, and the surface marker Ssea-1. Only one of two porcine iPSC lines differentiated into three germ layers both in vitro and in vivo. Western blot analysis showed that the porcine iPSCs were dependent on LIF or BMP-4 to sustain self-renewal and pluripotency. In conclusion, the results showed that human pluripotent factors could reprogram porcine ear fibroblasts into the pluripotent state. These cells may provide a useful source of cells that could be used for the treatment of degenerative and genetic diseases and agricultural research and application.
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Gyeonggi 441-706, Republic of Korea.
authors.forename: Dae-Jin
authors.initials: DJ
authors.lastname: Kwon
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Gyeonggi 441-706, Republic of Korea.
authors.forename: Hyelena
authors.initials: H
authors.lastname: Jeon
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Gyeonggi 441-706, Republic of Korea.
authors.forename: Keon Bong
authors.initials: KB
authors.lastname: Oh
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Gyeonggi 441-706, Republic of Korea.
authors.forename: Sun-A
authors.initials: SA
authors.lastname: Ock
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Gyeonggi 441-706, Republic of Korea.
authors.forename: Gi-Sun
authors.initials: GS
authors.lastname: Im
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Gyeonggi 441-706, Republic of Korea.
authors.forename: Sung-Soo
authors.initials: SS
authors.lastname: Lee
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Gyeonggi 441-706, Republic of Korea.
authors.forename: Seok Ki
authors.initials: SK
authors.lastname: Im
authors.affiliation: Research Center of Integrative Cellulomics, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
authors.forename: Jeong-Woong
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Department of Animal Biotechnology, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Sung-Jong
authors.initials: SJ
authors.lastname: Oh
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Gyeonggi 441-706, Republic of Korea.
authors.forename: Jin-Ki
authors.initials: JK
authors.lastname: Park
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Gyeonggi 441-706, Republic of Korea.
authors.forename: Seongsoo
authors.initials: S
authors.lastname: Hwang
mesh_terms: D000818:Animals; D055415:Bone Morphogenetic Protein 4 / Q000008:administration & dosage* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D006769:Hospitals, General; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D000090062:Kruppel-Like Factor 4; D053605:Leukemia Inhibitory Factor / Q000008:administration & dosage* / Q000378:metabolism; D008404:Massachusetts; D044968:Regenerative Medicine; D013552:Swine; D013556:Swine, Miniature
chemical_list: C525809:BMP4 protein, human; D055415:Bone Morphogenetic Protein 4; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor