title: Quorum sensing: a non-conventional target for antibiotic discovery.
abstract: Quorum sensing (QS) is known to regulate different functions viz. pathogenesis, biofilm formation, and host colonization, along with other functions by regulating bacterial virulence determinants. Therefore, QS is deemed to be an interesting target to modulate pathogenesis. Also, there have been global reports of continuous emergence of antibiotic-resistant microbes; hence, an alternative treatment that compliments antibiotic activity is highly desirable. One such approach is to look for QS inhibitors, which can quench the virulence phenotypes exerted by pathogenic bacteria and compliment antibiotic treatment. In the present study, Pseudomonas aeruginosa strain was used as the model organism which produces three pigments viz. pyocyanin, pyoverdin and pyorubin. Pyocyanin synthesis is reported to be QS dependent and is one of the virulence factors of P. aeruginosa. Hence, we envisage inhibition of pyocyanin pigment would indicate QS inhibition (QSI). Auto-inducers like N-(3-oxododecanoyl)-L-homoserine lactone (OdDHL/3-oxo-C12-HSL) and N-butyryl-L- homoserine lactone (BHL/C4-HSL) were used to enhance the pyocyanin pigment production by the model strain at different doses and time points. BHL, at 25 microM was found to be a better inducer of pyocyanin. Tannic acid (TA) was tested to suppress this pigment synthesis and it was found to be effective when assessed at different time points. About 5.12 mg/mL TA was found to be the optimum concentration at which pyocyanin was inhibited by 77.3%. Thus, we confirm that TA can be used as a QSI, either in its purest form or in the crude form found in various plant species, and could be considered for development to compliment antibiotic therapy.
authors.affiliation: Department of Natural Products, Anti-infective screening, Piramal Enterprises Limited, Mumbai, Maharashtra, India. varshasadashivnaik@gmail.com
authors.forename: Varsha
authors.initials: V
authors.lastname: Naik
authors.affiliation: Department of Natural Products, Anti-infective screening, Piramal Enterprises Limited, Mumbai, Maharashtra, India.
authors.forename: Girish
authors.initials: G
authors.lastname: Mahajan
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D055808:Drug Discovery / Q000379:methods*; D011550:Pseudomonas aeruginosa; D011710:Pyocyanine / Q000032:analysis / Q000037:antagonists & inhibitors*; D053038:Quorum Sensing; D013634:Tannins
chemical_list: D000900:Anti-Bacterial Agents; D013634:Tannins; D011710:Pyocyanine