title: [Expression of ZBTB8A in gastric cancer and its clinical significance].
abstract: OBJECTIVE
  To investigate the expression of ZBTB8A (zinc finger and BTB domain containing 8A) in gastric cancer tissues and its clinical significance.
  
  
  METHODS
  Level of ZBTB8A mRNA in human normal gastric cell line GES-1, human gastric cancer cell line SGC7901 and MGC803 was detected by real-time PCR. Levels of ZBTB8A mRNA and protein in cancer tissues, adjacent cancer tissues from 104 cases with primary gastric cancer and normal gastric mucosal tissues from 40 cases without malignant gastric diseases were detected by RT-PCR and immunohistochemistry, respectively. Association between ZBTB8A expression and clinicopathology was analyzed.
  
  
  RESULTS
  ZBTB8A mRNA expressions in SGC7901, MGC803 and GES-1 cells were 0.00138±0.00015, 0.00158±0.00021, 0.00036±0.000055, respectively, and differences among SGC7901, MGC803 and GES-1 were significant respectively (all P<0.05). ZBTB8A mRNA expression was significantly up-regulated in cancer tissues as compared to adjacent cancer tissues and normal tissues (0.0152±0.0126 vs. 0.0070±0.0061 and 0.0079±0.0036, all P>0.05), while no significant difference was found between adjacent cancer tissues and normal tissues (P>0.05). ZBTB8A expression was significantly associated with invasive depth, lymph node metastasis, tumor stage, and degree of adenocarcinoma differentiation (all P<0.05), but not with age, gender, histological type,gross type (all P>0.05).
  
  
  CONCLUSIONS
  ZBTB8A may be a potential carcinogenic factor in gastric carcinoma, and may also be involved in gastric adenocarcinoma cell differentiation, cancer invasion and metastasis.
authors.affiliation: Department of General Surgery, Huangdao Branch, Afiliated Hospital, Qingdao University School of Medicine, Qingdao 266555, China. cloudylucn@126.com.
authors.forename: Jin-hui
authors.initials: JH
authors.lastname: Wu
authors.forename: Yu-jie
authors.initials: YJ
authors.lastname: Feng
authors.forename: Zhi-jian
authors.initials: ZJ
authors.lastname: Sun
authors.forename: Ji-lin
authors.initials: JL
authors.lastname: Hu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.forename: Xian-xiang
authors.initials: XX
authors.lastname: Zhang
authors.forename: Mao-shen
authors.initials: MS
authors.lastname: Zhang
authors.forename: Bing-yuan
authors.initials: BY
authors.lastname: Zhang
authors.forename: Yun
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics; D013274:Stomach Neoplasms / Q000378:metabolism*
chemical_list: D051741:Kruppel-Like Transcription Factors; D012333:RNA, Messenger; C580776:ZBTB8A protein, human