title: Expression levels of ERCC1 and RRM1 mRNA and clinical outcome of advanced non-small cell lung cancer.
abstract: OBJECTIVE
  We conducted a study to understand the genetic effect of ERCC1 and RRM1 on the chemotherapy response and clinical outcome of Non-Small Cell Lung Cancer (NSCLC).
  
  
  METHODS
  The relative cDNA quantification for ERCC1 and RRM1 was conducted using a fluorescence-based real-time detection method among 294 NSCLC patients.
  
  
  RESULTS
  Compared with the internal reference gene β-action, the median levels of ERCC1 and RRM1 expression were 2.43×10(-2) and 0.11×10(-2), respectively. Our study showed response to platinum-containing regimen chemotherapy was high in those with high ERCC1 expression, and the OR (95% CI) were 1.73(1.06-2.81). An apparently high response to chemotherapy was decreased when patients were carrying both high expression of ERCC1 and RRM1, with OR (95% CI) of 2.57(1.21-4.90). Patients with high expression of ERCC1 were associated with a longer OS by multivariate Cox proportional hazards model. Moreover, those carrying both high levels of ERCC1 and RRM1 were seem to have a longer OS when compared with those with low expression (HR=0.31, 95% CI=0.13-0.62 for OS).
  
  
  CONCLUSIONS
  This observation could be used in personalized chemotherapy, increase the response rate and prolonged survival time, and could encourage to explore the predictive value of other genes.
authors.affiliation: Bi Jian-wei, Respiratory and Critical Care Medicine, the First Affiliated Hospital, Henan University of Science and Technology, Luoyang, China.
authors.forename: Bi
authors.initials: B
authors.lastname: Jian-Wei
authors.affiliation: Mao Yi-min, Respiratory and Critical Care Medicine, the First Affiliated Hospital, Henan University of Science and Technology, Luoyang, China.
authors.forename: Mao
authors.initials: M
authors.lastname: Yi-Min
authors.affiliation: Sun Yu-xia, Respiratory and Critical Care Medicine, the First Affiliated Hospital, Henan University of Science and Technology, Luoyang, China.
authors.forename: Sun
authors.initials: S
authors.lastname: Yu-Xia
authors.affiliation: Liu Shi-qing, Preventive Medicine, the Affiliated Qianfoshan Hospital, Shandong University, Ji'nan, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Shi-Qing
keywords: Advanced non-small cell lung cancer; ERCC1; Outcome; RRM1; mRNA