title: Deep sequencing-based transcriptional analysis of bovine mammary epithelial cells gene expression in response to in vitro infection with Staphylococcus aureus stains.
abstract: Staphylococcus aureus (S. aureus) is an important etiological organism in chronic and subclinical mastitis in lactating cows. Given the fundamental role the primary bovine mammary epithelial cells (pBMECs) play as a major first line of defense against invading pathogens, their interactions with S. aureus was hypothesized to be crucial to the establishment of the latter's infection process. This hypothesis was tested by investigating the global transcriptional responses of pBMECs to three S. aureus strains (S56,S178 and S36) with different virulent factors, using a tag-based high-throughput transcriptome sequencing technique. Approximately 4.9 million total sequence tags were obtained from each of the three S. aureus-infected libraries and the control library. Referenced to the control, 1720, 219, and 427 differentially expressed unique genes were identified in the pBMECs infected with S56, S178 and S36 S. aureus strains respectively. Gene ontology (GO) and pathway analysis of the S56-infected pBMECs referenced to those of the control revealed that the differentially expressed genes in S56-infected pBMECs were significantly involved in inflammatory response, cell signalling pathways and apoptosis. In the same vein, the clustered GO terms of the differentially expressed genes of the S178-infected pBMECs were found to comprise immune responses, metabolism transformation, and apoptosis, while those of the S36-infected pBMECs were primarily involved in cell cycle progression and immune responses. Furthermore, fundamental differences were observed in the levels of expression of immune-related genes in response to treatments with the three S. aureus strains. These differences were especially noted for the expression of important pro-inflammatory molecules, including IL-1α, TNF, EFNB1, IL-8, and EGR1. The transcriptional changes associated with cellular signaling and the inflammatory response in this study may reflect different immunomodulatory mechanisms that underlie the interaction between pBMECs and S. aureus strains during infection by the latter.
authors.affiliation: College of Life Sciences, Inner Mongolia University, Hohhot, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Inner Mongolia University, Hohhot, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Xiu
authors.affiliation: College of Life Sciences, Inner Mongolia University, Hohhot, China.
authors.forename: Qingliang
authors.initials: Q
authors.lastname: Hu
authors.affiliation: College of Life Sciences, Inner Mongolia University, Hohhot, China.
authors.forename: Xinjie
authors.initials: X
authors.lastname: Cui
authors.affiliation: College of Life Sciences, Inner Mongolia University, Hohhot, China.
authors.forename: Bingchun
authors.initials: B
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Inner Mongolia University, Hohhot, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Tao
authors.affiliation: College of Life Sciences, Inner Mongolia University, Hohhot, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Inner Mongolia University, Hohhot, China.
authors.forename: Jingging
authors.initials: J
authors.lastname: Wu
authors.affiliation: College of Life Sciences, Inner Mongolia University, Hohhot, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Life Sciences, Inner Mongolia University, Hohhot, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D002417:Cattle; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology*; D005260:Female; D005786:Gene Expression Regulation; D059014:High-Throughput Nucleotide Sequencing; D013203:Staphylococcal Infections / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D013211:Staphylococcus aureus
chemical_list: D016207:Cytokines