title: [Determination of ribavirin and amantadine in chicken by ultra performance liquid chromatography-tandem mass spectrometry].
abstract: A method for the determination of ribavirin and amantadine in chicken has been developed by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/ MS). After extracted by 1% (volume percentage) trichloroacetic acid solution-acetonitrile (1:1, v/v) and purified by a Supelco LC-SCX cartridge, the samples were loaded onto an Acquity UPLC BEH Hillic column (150 mm x 2.1 mm, 1.7 microm) and separated with gradient elution. The electrospray was operated in the positive mode and the samples were monitored by the multiple reaction monitoring (MRM) mode. The limits of quantification (LOQs, S/N = 10) of ribavirin and amantadine were 4.0 microg/kg. The calibration curves showed good linearity in the range of 10.0 - 100.0 microg/L, and the correlation coefficients (r(2)) were not lower than 0.99. When the spiked levels were 4.0, 8.0 and 20.0 microg/kg, the recoveries of ribavirin and amantadine in chicken ranged from 78% to 102.5%, with the relative standard deviations (RSDs) of 2.2% - 7.6%. The results indicate that the method is simple, rapid, sensitive and suitable for the qualitative and quantitative analyses of ribavirin and amantadine in chicken samples.
authors.affiliation: Technology Center of Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, China. yunh@bjciq.gov.cn
authors.forename: Huan
authors.initials: H
authors.lastname: Yun
authors.affiliation: Technology Center of Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, China.
authors.forename: Fengyun
authors.initials: F
authors.lastname: Cui
authors.affiliation: Technology Center of Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yan
authors.affiliation: Technology Center of Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Technology Center of Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: He
authors.affiliation: Technology Center of Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000547:Amantadine / Q000032:analysis*; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D012254:Ribavirin / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: D012254:Ribavirin; D000547:Amantadine