title: Effect of therapeutic hypothermia on DNA damage and neurodevelopmental outcome among term neonates with perinatal asphyxia: a randomized controlled trial.
abstract: OBJECTIVE
  To study the effect of therapeutic hypothermia (TH) on deoxyribonucleic acid (DNA) damage and the neurodevelopmental outcome in term babies with perinatal asphyxia.
  
  
  METHODS
  Babies in the hypothermia group were cooled for the first 72 h, using gel packs. Rectal temperature of 33-34°C was maintained. Blood sample was collected before, at 36 h and after completion of TH for assessment of comet assay and 8-hydroxy2-deoxyguanosine (8-OHdG). Infants were followed up till 12 months.
  
  
  RESULTS
  Baseline parameters were similar. After 72 h, the hypothermia group showed lower olive tail moment (12.88 ± 2.14) than the control group (22.16 ± 5.26) (p < 0.001). 8-HDG levels increased significantly in the control group (1252.87 ± 357.07) as compared to the hypothermia group (757.03 ± 198.49) (p < 0.001). Neurodevelopmental assessment at 12 months showed significantly low motor and mental developmental quotient in the control than hypothermia group.
  
  
  CONCLUSIONS
  TH reduces oxidative stress-induced DNA damage and improves neurodevelopmental outcome. <Trial registration No: CTRI/2011/10/002094>
authors.affiliation: Division of Neonatology, Department of Pediatrics, Jawaharlal Institute of Post-graduate Medical Education and Research (JIPMER), Pondicherry 605 006, India.
authors.forename: Bahubali D
authors.initials: BD
authors.lastname: Gane
authors.forename: Vishnu
authors.initials: V
authors.lastname: Bhat
authors.forename: Ramachandra
authors.initials: R
authors.lastname: Rao
authors.forename: S
authors.initials: S
authors.lastname: Nandhakumar
authors.forename: K T
authors.initials: KT
authors.lastname: Harichandrakumar
authors.forename: B
authors.initials: B
authors.lastname: Adhisivam
mesh_terms: D000328:Adult; D001238:Asphyxia Neonatorum / Q000150:complications / Q000628:therapy*; D020552:Comet Assay; D004249:DNA Damage; D003849:Deoxyguanosine; D002658:Developmental Disabilities / Q000209:etiology* / Q000517:prevention & control; D005260:Female; D005500:Follow-Up Studies; D006784:Hospitals, Teaching; D006801:Humans; D007036:Hypothermia, Induced / Q000009:adverse effects / Q000379:methods*; D020925:Hypoxia-Ischemia, Brain / Q000150:complications / Q000628:therapy*; D007194:India; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D012720:Severity of Illness Index; D016037:Single-Blind Method; D016896:Treatment Outcome
chemical_list: D003849:Deoxyguanosine
keywords: comet assay; gel packs; neurodevelopment; oxidative stress; perinatal asphyxia; therapeutic hypothermia