title: Arabidopsis mutants affecting oxylipin signaling in photo-oxidative stress responses.
abstract: Plant oxylipins derive from oxygenation of polyunsaturated fatty acids in thylakoid membranes and oxylipins such as jasmonic acid (JA) and 12-oxo-phytodienoic acid (OPDA) play important roles in adaptation to photo-oxidative stress. OPDA functions both as a JA precursor and as a biologically active signaling molecule that induces expression of a specific set of genes. These genes can be induced by OPDA in the JA-insensitive coronatine insensitive1 (coi1) mutant, suggesting that there is an alternative pathway for OPDA signaling, independent of COI1-dependent JA signaling. However, little is known about OPDA signaling in photo-oxidative stress responses. In this study, we isolated Arabidopsis mutants with constitutively enhanced expression from the OPDA-responsive HsfA2 promoter. We used deletion mapping and complementation analysis to identify one responsible gene as CATALASE2. Our results thus indicate that ROS-producing cellular metabolism links to OPDA signaling.
authors.affiliation: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Satoh
authors.affiliation: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan.
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Tokaji
authors.affiliation: Center for Ecological Research, Kyoto University, Shiga 520-2113, Japan; PRESTO, Japan Science and Technology Agency, Saitama 332-0012, Japan.
authors.forename: Atsushi J
authors.initials: AJ
authors.lastname: Nagano
authors.affiliation: Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Ikuko
authors.initials: I
authors.lastname: Hara-Nishimura
authors.affiliation: Department of Bioscience, Nagahama Institute of Bio-Science and Technology, Tamura 1266, Nagahama, Shiga, 526-0829 Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hayashi
authors.affiliation: Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-0867, Japan.
authors.forename: Mikio
authors.initials: M
authors.lastname: Nishimura
authors.affiliation: Center for Biological Resources and Informatics, Tokyo Institute of Technology, Yokohama 226-8503, Japan; Earth-Life Science Institute, Tokyo Institute of Technology, Tokyo 152-8550, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohta
authors.affiliation: Center for Biological Resources and Informatics, Tokyo Institute of Technology, Yokohama 226-8503, Japan; Earth-Life Science Institute, Tokyo Institute of Technology, Tokyo 152-8550, Japan. Electronic address: shmasuda@bio.titech.ac.jp.
authors.forename: Shinji
authors.initials: S
authors.lastname: Masuda
mesh_terms: D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics*; D003517:Cyclopentanes / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins / Q000235:genetics; D018869:Heat-Shock Response; D008027:Light; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D054883:Oxylipins / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D017384:Sequence Deletion; D015398:Signal Transduction; D020524:Thylakoids / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D004268:DNA-Binding Proteins; D005231:Fatty Acids, Unsaturated; C000620134:HSFA2 protein, Arabidopsis; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D014157:Transcription Factors; C025999:12-oxophytodienoic acid; C011006:jasmonic acid; C490276:CAT2 protein, Arabidopsis
keywords: Arabidopsis; Heat shock response; HsfA2; OPDA; Oxidative stress; Oxylipin; Reactive oxygen species