title: A novel Pseudomonas aeruginosa-derived effector cooperates with flagella to mediate the upregulation of interleukin 8 in human epithelial cells.
abstract: Infection with Pseudomonas aeruginosa results in a massive accumulation of neutrophils in response to prolonged and sustained expression of inflammatory mediators. The major chemokine associated with this excessive neutrophil recruitment is IL-8, the accumulation of which is a hallmark of cornea and cystic fibrosis airway inflammation. To date, several P. aeruginosa-associated and extracellular factors required for the stimulation of IL-8 expression have been identified. Here, we report a novel effector molecule, nucleoside diphosphate kinase (Ndk), which increases the expression of IL-8 by translocating into host cells. The induction appears to be dependent on both the kinase activity of Ndk and an additional bacterial factor, flagellin, via an NF-κB signaling pathway. This study demonstrates the role of a novel effector, Ndk, which is capable of inducing prominent inflammatory chemokine IL-8 expression with the aid of flagellin during P. aeruginosa infection.
authors.affiliation: Department of Biotechnology and Bioinformatics, Korea University, Sejong 339-700, Republic of Korea.
authors.forename: Yong-Jae
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Department of Biotechnology and Bioinformatics, Korea University, Sejong 339-700, Republic of Korea.
authors.forename: Se-Hwan
authors.initials: SH
authors.lastname: Paek
authors.affiliation: Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Shouguang
authors.initials: S
authors.lastname: Jin
authors.affiliation: Department of Biomedical Laboratory Science, Eulji University, Seongnam 461-713, Republic of Korea. Electronic address: bspark74@eulji.ac.kr.
authors.forename: Beom Seok
authors.initials: BS
authors.lastname: Park
authors.affiliation: Department of Biotechnology and Bioinformatics, Korea University, Sejong 339-700, Republic of Korea. Electronic address: haunhwan@korea.ac.kr.
authors.forename: Un-Hwan
authors.initials: UH
authors.lastname: Ha
mesh_terms: D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology*; D005407:Flagella / Q000378:metabolism*; D005408:Flagellin / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D020556:Neutrophil Infiltration; D009504:Neutrophils / Q000378:metabolism; D009701:Nucleoside-Diphosphate Kinase / Q000235:genetics / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D015854:Up-Regulation
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8; D016328:NF-kappa B; D005408:Flagellin; D009701:Nucleoside-Diphosphate Kinase
keywords: Epithelial cells; IL-8; Pseudomonas aeruginosa