title: Proteomic analysis of the effect of diclazuril on second-generation merozoites of Eimeria tenella.
abstract: Diclazuril has long been used as an effective benzeneacetonitrile anticoccidial for the control of Eimeria tenella that causes coccidiosis. However, the molecular mechanism underlying the anticoccidial effects of diclazuril remains elusive. In this study, a proteomic analysis of the effect of diclazuril on second-generation merozoites of E. tenella was performed. Using two-dimensional gel electrophoresis and real-time quantitative polymerase chain reaction (RT-PCR), 13 target proteins were found to be significantly affected by diclazuril treatment, with 11 of these proteins being identified as annotated proteins from E. tenella or other Apicomplexa parasites. These proteins contribute to various functions, including metabolism, protein synthesis, and host cell invasion. Using RT-PCR, we identified the potential pattern of transcriptional regulation induced by diclazuril, and we suggest some promising targets for the intervention of E. tenella infection.
authors.affiliation: Key Laboratory of Animal Parasitology, Ministry of Agriculture, People's Republic of China, Shanghai Veterinary Research Institute, Chinese Academy of Agriculture Science, Minhang, Shanghai, 200241, People's Republic of China.
authors.forename: Xiao-jiong
authors.initials: XJ
authors.lastname: Shen
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Jian-jun
authors.initials: JJ
authors.lastname: Fu
authors.forename: Ke-yu
authors.initials: KY
authors.lastname: Zhang
authors.forename: Xiao-yang
authors.initials: XY
authors.lastname: Wang
authors.forename: Yin-chun
authors.initials: YC
authors.lastname: Liu
authors.forename: Hua-jing
authors.initials: HJ
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Fan
authors.forename: Cheng-zhong
authors.initials: CZ
authors.lastname: Fei
authors.forename: Fei-qun
authors.initials: FQ
authors.lastname: Xue
mesh_terms: D000818:Animals; D002645:Chickens / Q000469:parasitology; D003049:Coccidiostats / Q000494:pharmacology*; D016786:Eimeria tenella / Q000187:drug effects* / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D052940:Merozoites / Q000187:drug effects; D009570:Nitriles / Q000494:pharmacology*; D020543:Proteome / Q000032:analysis*; D014227:Triazines / Q000494:pharmacology*
chemical_list: D003049:Coccidiostats; D009570:Nitriles; D020543:Proteome; D014227:Triazines; C057884:diclazuril