title: Selection of oleaginous yeasts with high lipid productivity for practical biodiesel production.
abstract: The lipid-accumulating ability of 500 yeast strains isolated in Japan was evaluated. Primary screening revealed that 31 strains were identified as potential lipid producers, from which 12 strains were cultivated in a medium containing 3% glucose. It was found that JCM 24511 accumulated the highest lipid content, up to 61.53%, while JCM 24512 grew the fastest. They were tentatively identified as Cryptococcus sp. and Cryptococcus musci, respectively. The maximum lipid concentration of 1.49g/L was achieved by JCM 24512. Similarly, JCM 24511 also achieved a high lipid production of 1.37g/L. High lipid productivity is the most important characteristic of oleaginous yeasts from the viewpoint of practical production. Among the strains tested here, JCM 24512 had the best lipid productivity, 0.37g/L/day. The results show that the isolated yeasts could be promising candidates for biodiesel production.
authors.affiliation: Research Division of Microbial Sciences, Kyoto University, Kitashirakawa Oiwake-Cho, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Tanimura
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, Koyadai, Tuskuba, Ibaraki 305-0074, Japan.
authors.forename: Masako
authors.initials: M
authors.lastname: Takashima
authors.affiliation: Department of Microbiology, Meiji Pharmaceutical University, Tokyo 204-8588, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Sugita
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, Koyadai, Tuskuba, Ibaraki 305-0074, Japan.
authors.forename: Rikiya
authors.initials: R
authors.lastname: Endoh
authors.affiliation: Research Division of Microbial Sciences, Kyoto University, Kitashirakawa Oiwake-Cho, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Minako
authors.initials: M
authors.lastname: Kikukawa
authors.affiliation: Research Division of Microbial Sciences, Kyoto University, Kitashirakawa Oiwake-Cho, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Shino
authors.initials: S
authors.lastname: Yamaguchi
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-Cho, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Sakuradani
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-Cho, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Ogawa
authors.affiliation: Research Division of Microbial Sciences, Kyoto University, Kitashirakawa Oiwake-Cho, Sakyo-ku, Kyoto 606-8502, Japan. Electronic address: shimaj@kais.kyoto-u.ac.jp.
authors.forename: Jun
authors.initials: J
authors.lastname: Shima
mesh_terms: D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D003454:Cryptococcus / Q000378:metabolism*; D005062:European Union; D005227:Fatty Acids / Q000378:metabolism; D005229:Fatty Acids, Monounsaturated; D031286:Jatropha / Q000737:chemistry; D007700:Kinetics; D008055:Lipids / Q000096:biosynthesis*; D010938:Plant Oils / Q000378:metabolism; D000074262:Rapeseed Oil; D013997:Time Factors; D014481:United States
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D008055:Lipids; D010938:Plant Oils; D000074262:Rapeseed Oil
keywords: Cryptococcus musci; Cryptococcus podzolicus; Fatty acids; Lipid productivity; Oleaginous yeast