title: Microvascular disorders in obese Zucker rats are restored by a rice bran diet.
abstract: OBJECTIVE
  Nutritional-based approaches aimed to prevent microvascular dysfunction associated to obesity present potential advantages over pharmacological strategies. Our aim was to test whether a rice bran enzymatic extract (RBEE)-supplemented diet could attenuate microvascular alterations in obese rats.
  
  
  RESULTS
  Lean and obese Zucker rats were fed standard diet supplemented or not with 1% and 5% RBEE for 20 weeks. Functional studies were performed in small mesenteric arteries in isometric myograph. Immunoblotting and fluorescence studies were made in arterial homogenates and arterial sections, respectively. RBEE-supplementation restored microvascular function in obese rats through a marked increase in NO and endothelial-derived hyperpolarizing factor contribution by up-regulation of eNOS and calcium-activated potassium channels expression, respectively, in association to a substantial reduction of microvascular inflammation and superoxide anion formation. These data agrees with the beneficial actions of RBEE on dyslipidemia, hyperinsulinemia and hypertension in obesity.
  
  
  CONCLUSIONS
  The multi-factorial properties of RBEE-diet, especially for restoring the function of small resistance arteries shows this dietary-based approach to be a promising candidate for prevention of microvascular alterations in obesity, which are crucial in cardiovascular events in obese subjects.
authors.affiliation: Department of Pharmacology, School of Pharmacy, University of Seville, Seville, Spain.
authors.forename: M L
authors.initials: ML
authors.lastname: Justo
authors.affiliation: Department of Pharmacology, School of Pharmacy, University of Seville, Seville, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Claro
authors.affiliation: Departament de Farmacologia, Terapèutica i Toxicologia, Facultat de Medicina, Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Vila
authors.affiliation: Department of Pharmacology, School of Pharmacy, University of Seville, Seville, Spain.
authors.forename: M D
authors.initials: MD
authors.lastname: Herrera
authors.affiliation: Department of Pharmacology, School of Pharmacy, University of Seville, Seville, Spain. Electronic address: rodriguezr@us.es.
authors.forename: R
authors.initials: R
authors.lastname: Rodriguez-Rodriguez
mesh_terms: D052242:Adiponectin / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure; D008076:Cholesterol, HDL / Q000097:blood; D004032:Diet; D004043:Dietary Fiber / Q000008:administration & dosage*; D050171:Dyslipidemias / Q000178:diet therapy; D006946:Hyperinsulinism / Q000178:diet therapy; D006973:Hypertension / Q000178:diet therapy*; D007328:Insulin / Q000097:blood; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D009569:Nitric Oxide / Q000097:blood; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000378:metabolism; D009765:Obesity / Q000178:diet therapy*; D012275:Oryza / Q000737:chemistry*; D051381:Rats; D011924:Rats, Zucker; D014280:Triglycerides / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D015854:Up-Regulation
chemical_list: D052242:Adiponectin; D001786:Blood Glucose; D008076:Cholesterol, HDL; D004043:Dietary Fiber; D007328:Insulin; D053583:Interleukin-1beta; D015850:Interleukin-6; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat
keywords: Mesenteric artery; Microvascular function; Obese Zucker rat; Obesity; Rice bran