title: Creation of artificial cellulosomes on DNA scaffolds by zinc finger protein-guided assembly for efficient cellulose hydrolysis.
abstract: For the first time, artificial cellulosome structures were created on DNA scaffolds based on zinc finger protein (ZFP)-guided assembly. These resulting two-component cellulosome structures exhibited enhancement in cellulose hydrolysis compared to the non-complexed mixture depending on the number of CBMs and cellulases assembled.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, Delaware 19716, USA. wilfred@udel.edu.
authors.forename: Qing
authors.initials: Q
authors.lastname: Sun
authors.forename: Bhawna
authors.initials: B
authors.lastname: Madan
authors.forename: Shen-Long
authors.initials: SL
authors.lastname: Tsai
authors.forename: Matthew P
authors.initials: MP
authors.lastname: DeLisa
authors.forename: Wilfred
authors.initials: W
authors.lastname: Chen
mesh_terms: D001665:Binding Sites; D002482:Cellulose / Q000737:chemistry*; D044603:Cellulosomes / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D006868:Hydrolysis; D011506:Proteins / Q000737:chemistry*; D016335:Zinc Fingers
chemical_list: D004800:Enzymes, Immobilized; D011506:Proteins; D002482:Cellulose; D004247:DNA