title: A novel promising Trichoderma harzianum strain for the production of a cellulolytic complex using sugarcane bagasse in natura.
abstract: Brazil is a major producer of agro-industrial residues, such as sugarcane bagasse, which could be used as raw material for microbial production of cellulases as an important strategy for the development of sustainable processes of second generation ethanol production. For this purpose, this work aimed at screening for glycosyl hydrolase activities of fungal strains isolated from the Brazilian Cerrado. Among 13 isolates, a Trichoderma harzianum strain (L04) was identified as a promising candidate for cellulase production when cultured on in natura sugarcane bagasse. Strain L04 revealed a well-balanced cellulolytic complex, presenting fast kinetic production of endoglucanases, exoglucanases and β-glucosidases, achieving 4,022, U.L(-1) (72 h), 1,228 U.L(-1) (120 h) and 1,968 U.L(-1) (48 h) as the highest activities, respectively. About 60% glucose yields were obtained from sugarcane bagasse after 18 hours hydrolysis. This new strain represents a potential candidate for on-site enzyme production using sugarcane bagasse as carbon source.
authors.affiliation: Centro de Biotecnologia Molecular, Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Bruno
authors.initials: B
authors.lastname: Benoliel
authors.affiliation: Centro de Biotecnologia Molecular, Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Fernando Araripe Gonçalves
authors.initials: FA
authors.lastname: Torres
authors.affiliation: Centro de Biotecnologia Molecular, Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil ; Laboratório de Biologia Molecular, Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF 70910-900 Brazil.
authors.forename: Lidia Maria Pepe
authors.initials: LM
authors.lastname: de Moraes
keywords: Brazilian Cerrado; Cellulases; Sugarcane bagasse; Trichoderma harzianum