title: [Application of human oocyte morphometric parameters in assessment of fertilization and embryo development].
abstract: OBJECTIVE
  To investigate the correlation of human oocyte morphometric parameters with fertilization and embryo development in the intracytoplasmic sperm injection (ICSI) cycles.
  
  
  METHODS
  The morphometric parameters of oocytes collected and submitted to evaluation using OCTAX Eye-ware software just before ICSI. Oocyte diameter (OD), perivitelline space width (PSW), zonapellucida thickness (ZPT) and the shape of first polar body (FPB) (intact or fragmented) were analyzed. A stepwise multivariate Logistic regression was used to test the association between the morphometric parameters and fertilization and embryo development.
  
  
  RESULTS
  In the study, 436 oocytes were measured and 370 were fertilized (84.9%), 225 fertilized oocytes were developed to high-quality embryos (60.8%). ZPT and PSW were associated with fertilization. The oocytes fertilized had thicker ZPT [(18.0±2.3) μm vs. (16.9±2.7) μm] and wider PSW [(14.4±3.3) μm vs. (13.2±3.9) μm]. The OD and shape of FPB were associated with embryos development. The oocytes developed to high-quality embryos had larger OD [(116.6±3.7) μm vs. (114.7±3.6) μm] and more intact FPB (86.2% vs. 66.7%).
  
  
  CONCLUSIONS
  The morphometric parameters of oocytes can indicate fertilization and embryo development.
authors.affiliation: Reproductive Medical Center, Ruijin Hospital, Shanghai Jiaotong University of Medicine, Shanghai 200025, China.
authors.forename: Yi-juan
authors.initials: YJ
authors.lastname: Sun
authors.forename: Rui-huan
authors.initials: RH
authors.lastname: Gu
authors.forename: Xiao-wei
authors.initials: XW
authors.lastname: Lu
authors.forename: Shen
authors.initials: S
authors.lastname: Zhao
authors.forename: Yun
authors.initials: Y
authors.lastname: Feng
mesh_terms: D000328:Adult; D048430:Cell Shape; D048429:Cell Size; D004477:Ectogenesis; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007246:Infertility / Q000628:therapy; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000648:ultrastructure*; D020554:Sperm Injections, Intracytoplasmic