title: Does bromocriptine play a role in decreasing oxidative stress for early weaned programmed obesity?
abstract: OBJECTIVE
  Studies have demonstrated that early weaning can promote metabolic syndrome during adulthood and that obesity increases oxidative stress. Thus, we aimed to evaluate redox status in a pharmacological early weaning rodent model programmed for metabolic syndrome at adulthood.
  
  
  METHODS
  Lactating dams were randomly assigned into 2 groups: the early weaning group (BRO), which was treated intraperitoneally with bromocriptine (1 mg/day) to inhibit prolactin secretion for the last 3 days of lactation, and the control group (C), which received the BRO diluent for the same time period. The offspring were killed at 90 (PN90) and 180 (PN180) days after birth.
  
  
  RESULTS
  Early weaning induced greater visceral adiposity and dyslipidemia. At PN90, the BRO offspring showed glucose intolerance with normoinsulinemia and increased plasma and liver superoxide dismutase, and liver glutathione peroxidase activities, which reduced the liver malondialdehyde but not the increased plasma malondialdehyde levels. However, the BRO offspring showed insulin resistance at PN180 and increased plasma glutathione peroxidase, liver superoxide dismutase, and catalase activities. These changes reduced the plasma and liver malondialdehyde levels, which aided in hepatocyte architecture preservation. Additionally, we observed that sirtuin 1 was overexpressed in the BRO group at PN90, but the increased expression was not maintained through PN180, which suggests unfavorable metabolic conditions in the older offspring.
  
  
  CONCLUSIONS
  Despite the observed obesity and glucose homeostasis dysfunction, our data suggest that the early weaning programming induced by bromocriptine can improve the offspring's redox status and may prevent liver damage during adulthood.
authors.affiliation: Department of Physiological Sciences, Roberto Alcantara Gomes Biology Institute, Brazil.
authors.forename: Nayara
authors.initials: N
authors.lastname: Peixoto-Silva
authors.affiliation: Department of Physiological Sciences, Roberto Alcantara Gomes Biology Institute, Brazil.
authors.forename: Ellen P S
authors.initials: EP
authors.lastname: Conceição
authors.affiliation: Department of Physiological Sciences, Roberto Alcantara Gomes Biology Institute, Brazil.
authors.forename: Janaine C
authors.initials: JC
authors.lastname: Carvalho
authors.affiliation: Department of Physiological Sciences, Roberto Alcantara Gomes Biology Institute, Brazil.
authors.forename: Natália S
authors.initials: NS
authors.lastname: Lima
authors.affiliation: Laboratory of Lipids, School of Medicine, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: José Firmino
authors.initials: JF
authors.lastname: Nogueira-Neto
authors.affiliation: Department of Physiological Sciences, Roberto Alcantara Gomes Biology Institute, Brazil.
authors.forename: Elaine
authors.initials: E
authors.lastname: de Oliveira
authors.affiliation: Department of Physiological Sciences, Roberto Alcantara Gomes Biology Institute, Brazil.
authors.forename: Egberto G
authors.initials: EG
authors.lastname: Moura
authors.affiliation: Department of Physiological Sciences, Roberto Alcantara Gomes Biology Institute, Brazil. Electronic address: pclisboa@uerj.br.
authors.forename: Patricia C
authors.initials: PC
authors.lastname: Lisboa
mesh_terms: D050154:Adiposity; D000818:Animals; D001971:Bromocriptine / Q000494:pharmacology*; D050171:Dyslipidemias / Q000209:etiology; D005260:Female; D005947:Glucose / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D007333:Insulin Resistance; D050152:Intra-Abdominal Fat / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008107:Liver Diseases / Q000209:etiology / Q000517:prevention & control; D008297:Male; D009765:Obesity / Q000209:etiology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D011388:Prolactin / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014886:Weaning
chemical_list: D001971:Bromocriptine; D011388:Prolactin; D005947:Glucose
keywords: Antioxidant system; Bromocriptine; Early weaning; Liver disease; Obesity