title: Canine amino acid transport system Xc(-): cDNA sequence, distribution and cystine transport activity in lens epithelial cells.
abstract: The cystine transport activity of a lens epithelial cell line originated from a canine mature cataract was investigated. The distinct cystine transport activity was observed, which was inhibited to 28% by extracellular 1 mM glutamate. The cDNA sequences of canine cysteine/glutamate exchanger (xCT) and 4F2hc were determined. The predicted amino acid sequences were 527 and 533 amino acid polypeptides, respectively. The amino acid sequences of canine xCT and 4F2hc showed high similarities (>80%) to those of humans. The expression of xCT in lens epithelial cell line was confirmed by western blot analysis. RT-PCR analysis revealed high level expression only in the brain, and it was below the detectable level in other tissues.
authors.affiliation: Veterinary Teaching Hospital, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuou-ku, Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Maruo
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kanemaki
authors.forename: Ken
authors.initials: K
authors.lastname: Onda
authors.forename: Reiichiro
authors.initials: R
authors.lastname: Sato
authors.forename: Nobuteru
authors.initials: N
authors.lastname: Ichihara
authors.forename: Hideharu
authors.initials: H
authors.lastname: Ochiai
mesh_terms: D000595:Amino Acid Sequence; D026923:Amino Acid Transport Systems, Basic / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D003553:Cystine / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004285:Dogs; D027282:Fusion Regulatory Protein 1, Heavy Chain; D018698:Glutamic Acid; D007908:Lens, Crystalline / Q000502:physiology*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D026923:Amino Acid Transport Systems, Basic; D017931:DNA Primers; D018076:DNA, Complementary; D027282:Fusion Regulatory Protein 1, Heavy Chain; C545989:SLC3A2 protein, human; D018698:Glutamic Acid; D003553:Cystine