title: Polyphenolic compounds from callus cultures of Iris pseudacorus.
abstract: A callus culture of Iris pseudacorus L. (Iridaceae) was established from plant leaves using a modified Murashige and Skoog medium. A derivative of cinnamic acid (lavandoside) (1), a neolignan (dehydrodiconiferyl alcohol-4-O-beta-D-glucopyranoside) (2) as well as three isoflavonoids, tectoridin (3), tectorigenin (4), and iristectorigenin A (5) were isolated from the callus culture. Under normal conditions, the calli accumulated 0.4% DW of polyphenols. The addition of phenylalanine to a concentration of 1 mM resulted in a 1.5-fold increase in isoflavonoid production, allowing the accumulation of 0.69% of polyphenols in the callus dry weight. Tectorigenin, a promising chemotherapeutic and chemopreventive agent for the treatment of carcinomas, was produced in I. pseudacorus calli in high quantities (0.3% DW).
authors.affiliation: G. B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia. tarbeeva1988@mail.ru
authors.forename: Darya V
authors.initials: DV
authors.lastname: Tarbeeva
authors.affiliation: G. B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia.
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Fedoreyev
authors.affiliation: G. B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia.
authors.forename: Marina V
authors.initials: MV
authors.lastname: Veselova
authors.affiliation: G. B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia.
authors.forename: Anatoliy I
authors.initials: AI
authors.lastname: Kalinovskiy
authors.affiliation: lnstitute of Biology and Soil Science, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia.
authors.forename: Ludmila D
authors.initials: LD
authors.lastname: Seletskaya
authors.affiliation: lnstitute of Biology and Soil Science, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia.
authors.forename: Tamara I
authors.initials: TI
authors.lastname: Mazurok
authors.affiliation: lnstitute of Biology and Soil Science, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia.
authors.forename: Victor P
authors.initials: VP
authors.lastname: Bulgakov
mesh_terms: D002478:Cells, Cultured; D046508:Culture Techniques; D031323:Iris Plant / Q000737:chemistry*; D007529:Isoflavones / Q000302:isolation & purification*; D017705:Lignans / Q000302:isolation & purification; D059808:Polyphenols / Q000302:isolation & purification*
chemical_list: D007529:Isoflavones; D017705:Lignans; D059808:Polyphenols; C120039:tectorigenin; C120040:tectoridin