title: Lectin histochemistry of palatine glands in the developing rat.
abstract: This study examined the binding pattern of lectins, soybean agglutinin (SBA), Dolichos biflorus agglutinin (DBA), Vicia villosa agglutinin (VVA), Ulex europaeus agglutinin-I (UEA-I), peanut agglutinin (PNA), wheat germ agglutinin (WGA), and succinylated WGA (sucWGA) in the developing rat palatine glands. In adult rats, heterogeneous lectin binding patterns were revealed between the anterior and posterior portions of palatine glands, as DBA, VVA, and WGA were bound more intensely and broadly in the posterior portion. SBA, PNA, and sucWGA showed far less reactivity in the anterior than in the posterior portion. At embryonic day 18 (E18), weak labeling was observed with UEA-I and WGA at the basal membrane of terminal buds, UEA-I and PNA labeled the epithelial cord, and there was no apparent binding for SBA, DBA, VVA, and sucWGA. At E20, after acinar lumenization, all lectins were detected at the acinar cell basal membranes. After birth, all lectins detectably labeled at the mucous cell apical membranes and progressively, with maturation, extended from the apical to basal portions of the cytoplasm. Apparent serous cells were observed around postnatal day 10 (PN10) and bound UEA-I. Lectins reached peak reactivity at PN21 and the binding patterns became identical to those of adults around PN28.
authors.affiliation: Department of Orthodontic and Dentofacial Orthopedics, Tohoku University Graduate School of Dentistry, Sendai, Japan; Department of Oral Anatomy and Developmental Biology, Osaka University Graduate School of Dentistry, Osaka, Japan.
authors.forename: Zaki
authors.initials: Z
authors.lastname: Hakami
authors.affiliation: Department of Orthodontic and Dentofacial Orthopedics, Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Kitaura
authors.affiliation: Department of Oral Anatomy and Developmental Biology, Osaka University Graduate School of Dentistry, Osaka, Japan.
authors.forename: Shiho
authors.initials: S
authors.lastname: Honma
authors.affiliation: Department of Oral Anatomy and Developmental Biology, Osaka University Graduate School of Dentistry, Osaka, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Wakisaka
authors.affiliation: Department of Orthodontic and Dentofacial Orthopedics, Tohoku University Graduate School of Dentistry, Sendai, Japan. Electronic address: t-yamamo@m.tohoku.ac.jp.
authors.forename: Teruko
authors.initials: T
authors.lastname: Takano-Yamamoto
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D005260:Female; D006651:Histocytochemistry / Q000379:methods; D009092:Mucous Membrane / Q000254:growth & development / Q000378:metabolism*; D037121:Plant Lectins / Q000378:metabolism*; D051381:Rats; D014886:Weaning
chemical_list: D037121:Plant Lectins
keywords: Development; Glycoproteins; Lectins; Palatine glands; Rat