title: Protein immobilization on nanoporous silicon functionalized by RF activated plasma polymerization of Acrylic Acid.
abstract: Plasma Enhanced Chemical Vapor Deposition (PECVD) technique is used to polymerize Acrylic Acid for the surface functionalization of porous silicon samples with different pore dimensions. The polymer shows free COOH groups also at the pores inner surface, suitable for the immobilization of fluorescent labeled Protein A. The stability of the polymer, its role in the protection from aging of the porous matrix and the efficiency of the functionalization for the binding of protein A have been characterized by ATR-FTIR, SEM, Optical Contact Angle and Fluorescence Microscopy. The polymerization process is well controllable and suitable for the functionalization of porous silicon leaving free carboxylic groups at the surface ready for the immobilization of biochemical species for sensing applications.
authors.affiliation: Dipartimento di Scienza Applicata e Tecnologia, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Torino, Italy. Electronic address: paola.rivolo@polito.it.
authors.forename: Paola
authors.initials: P
authors.lastname: Rivolo
authors.affiliation: Dipartimento di Scienza Applicata e Tecnologia, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Torino, Italy.
authors.forename: Sara Maria
authors.initials: SM
authors.lastname: Severino
authors.affiliation: Dipartimento di Scienza Applicata e Tecnologia, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Torino, Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Ricciardi
authors.affiliation: Dipartimento di Scienza Applicata e Tecnologia, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Torino, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Frascella
authors.affiliation: Dipartimento di Scienza Applicata e Tecnologia, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Torino, Italy. Electronic address: francesco.geobaldo@polito.it.
authors.forename: Francesco
authors.initials: F
authors.lastname: Geobaldo
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D015374:Biosensing Techniques; D005456:Fluorescent Dyes; D055767:Immobilized Proteins / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D058626:Plasma Gases / Q000737:chemistry; D058105:Polymerization; D016062:Porosity; D011806:Quinolinium Compounds; D012825:Silicon / Q000737:chemistry*; D013205:Staphylococcal Protein A / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000179:Acrylates; C481052:Alexa fluor 546; D005456:Fluorescent Dyes; D055767:Immobilized Proteins; D058626:Plasma Gases; D011806:Quinolinium Compounds; D013205:Staphylococcal Protein A; C036658:acrylic acid; D012825:Silicon
keywords: Acrylic Acid; Plasma polymerization; Porous silicon; Protein covalent binding; Surface functionalization