title: Accumulation of advanced glycation end-products in human dentine.
abstract: Cross-linking of collagen by Advanced Glycation End-products (AGEs) occurs by non-enzymatic glycation (Maillard reaction). The purpose of this study was to examine whether AGEs are formed in human dentinal collagen, and to consider any possible influence of AGEs on dentinal physiology. Mechanical characteristics, fluorescence spectra and immunohistochemical analyses of demineralized dentine sections from young subjects were compared with those of aged ones. The same investigations were performed with young dentine artificially glycated by incubation in 0.1M ribose solution. Indentation measurement indicated that the sections from aged dentine were mechanically harder than those from young dentine. The hardness of young dentine increased after incubation in ribose solution. Fluorescence peak wavelength of the young dentine was shorter than that of the aged one, but shifted towards the peak wavelength of the aged one after incubation in ribose solution. These changes were considered to be due to accumulation of AGEs. Existence of AGEs in dentinal collagen was confirmed by immunohistochemical analysis. The obtained results suggest that AGEs accumulation occurs in dentinal collagen and is affected by both human age and physiological conditions such as glucose level in blood because dentinal collagen receives nourishment via dental pulp and tubules.
authors.affiliation: Division for Interdisciplinary Dentistry, Osaka University Dental Hospital, Japan. Electronic address: miura_j@dent.osaka-u.ac.jp.
authors.forename: Jiro
authors.initials: J
authors.lastname: Miura
authors.affiliation: Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Japan.
authors.forename: Kantaro
authors.initials: K
authors.lastname: Nishikawa
authors.affiliation: Division for Interdisciplinary Dentistry, Osaka University Dental Hospital, Japan.
authors.forename: Mizuho
authors.initials: M
authors.lastname: Kubo
authors.affiliation: Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Japan.
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Fukushima
authors.affiliation: Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Hashimoto
authors.affiliation: Division for Interdisciplinary Dentistry, Osaka University Dental Hospital, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Takeshige
authors.affiliation: Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Araki
mesh_terms: D000293:Adolescent; D000328:Adult; D003432:Cross-Linking Reagents; D003804:Dentin / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D005453:Fluorescence; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006244:Hardness; D006801:Humans; D007150:Immunohistochemistry; D015416:Maillard Reaction; D008297:Male; D008854:Microscopy, Electron; D008964:Molar, Third
chemical_list: D003432:Cross-Linking Reagents; D017127:Glycation End Products, Advanced
keywords: Ageing; Browning; Collagen; Fluorescence; Glycation; Human dentine