title: Effect of phase 1 periodontal therapy on gingival crevicular fluid levels of matrix metalloproteinases-3 and -13 in chronic periodontitis patients.
abstract: OBJECTIVE
  The aim of the present study was to estimate the gingival crevicular fluid (GCF) levels of matrix metalloproteinases (MMP)-3 and -13 in periodontally-healthy controls and chronic periodontitis (CP) patients, and also to investigate the effect of phase 1 periodontal therapy on MMP-3 and -13 levels in CP patients.
  
  
  METHODS
  Fifty-five systemically-healthy patients were divided into two groups: group 1 (healthy) and group 2 (CP). The recording of clinical parameters and GCF sampling was done at baseline for both groups and again at 6 weeks post-therapy for group 2. The MMP level was determined by ELISA.
  
  
  RESULTS
  A significant increase in the mean MMP-3 and -13 was found between healthy and CP patients. There was a statistically-significant reduction of GCF MMP-3 and -13 concentration after periodontal therapy in the CP group. A positive correlation was found between clinical parameters and GCF MMP-3 and -13 levels.
  
  
  CONCLUSIONS
  A lower concentration of GCF MMP-3 and -13 was found in healthy patients, and a higher concentration was noted for CP patients, which was reduced after periodontal therapy. This indicates the important role played by these MMP in periodontal destruction. Thus, MMP-3 and -13 could be used as inflammatory biomarkers in diagnosing periodontal disease severity.
authors.affiliation: Department of Periodontics, Bapuji Dental College and Hospital, Davangere, India.
authors.forename: Deepali D
authors.initials: DD
authors.lastname: Pawar
authors.forename: Dhoom S
authors.initials: DS
authors.lastname: Mehta
mesh_terms: D000328:Adult; D016301:Alveolar Bone Loss / Q000201:enzymology / Q000628:therapy; D015415:Biomarkers / Q000032:analysis; D055113:Chronic Periodontitis / Q000201:enzymology / Q000628:therapy*; D003774:Dental Plaque Index; D005260:Female; D005500:Follow-Up Studies; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000032:analysis*; D019278:Matrix Metalloproteinase 3 / Q000032:analysis*; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000201:enzymology / Q000628:therapy; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000201:enzymology / Q000628:therapy; D055815:Young Adult
chemical_list: D015415:Biomarkers; D018836:Inflammation Mediators; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3
keywords: chronic periodontitis; gingival crevicular fluid; metalloproteinase-13; metalloproteinase-3; periodontal therapy