title: Preparation of molecularly imprinted polymer for use as SPE adsorbent for the simultaneous determination of five sulphonylurea herbicides by HPLC.
abstract: A high selective pre-treatment method for the analysis of sulphonylurea herbicides (SUHs) in rice grain samples based on molecularly imprinted solid-phase extraction (MISPE) was developed. The molecularly imprinted polymers (MIPs) were synthesised with high adsorption capacity and suitable particle size using pyrazosulphuron ethyl (PS) as the template and by screening three porogens and porogen volumes. The resulting MIPs showed high selectivity for PS. The MISPE procedure was developed for the purification and enrichment of PS, bensulphuron methyl (BSM), tribenuron methyl (TBM), metsulphuron methyl (MSM) and nicosulphuron (NS) from a rice grain sample prior to reversed-phase high-performance liquid chromatography (HPLC) analysis. Average recoveries of the PS, NS, MSM and BSM were 95.6%, 88.6%, 87.8% and 89.4%, respectively, but the recovery of TBM was lower (48.2%). This pre-treatment methodology for extracting SUHs was simple and cleaner extractions. The limits of detection ranged from 10.1 to 50.0ngL(-)(1). Five local rice grain samples were analysed by HPLC-MISPE, and PS was detected in all five samples, while BSM was detected in one sample.
authors.affiliation: Jiangxi Key Laboratory of Natural Products and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China.
authors.forename: Kaijie
authors.initials: K
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Gu
authors.affiliation: Jiangxi Key Laboratory of Natural Products and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China.
authors.forename: Qiushui
authors.initials: Q
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Shangwei
authors.initials: S
authors.lastname: Chen
authors.affiliation: Jiangxi Key Laboratory of Natural Products and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China.
authors.forename: Leiyan
authors.initials: L
authors.lastname: Wu
authors.affiliation: Jiangxi Key Laboratory of Natural Products and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China. Electronic address: xjhtkjxnh@163.com.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Xiong
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006540:Herbicides / Q000737:chemistry* / Q000302:isolation & purification; D054802:Molecular Imprinting; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D013453:Sulfonylurea Compounds / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D006540:Herbicides; D011108:Polymers; D013453:Sulfonylurea Compounds
keywords: Molecularly imprinted polymers; Pyrazosulphuron ethyl; Rice grain; Solid-phase extraction; Sulphonylurea herbicides