title: The use of coenzyme Q0 as a template in the development of a molecularly imprinted polymer for the selective recognition of coenzyme Q10.
abstract: In this work, a novel molecularly imprinted polymer (MIP) for use as a solid phase extraction sorbent was developed for the determination of coenzyme Q10 (CoQ10) in liver extract. CoQ10 is an essential cofactor in mitochondrial oxidative phosphorylation and a powerful antioxidant agent found in low concentrations in biological samples. This fact and its high hydrophobicity make the analysis of CoQ10 technically challenging. Accordingly, a MIP was synthesised using coenzyme Q0 as the template, methacrylic acid as the functional monomer, acetonitrile as the porogen, ethylene glycol dimethacrylate as the crosslinker and benzoyl peroxide as the initiator. Various parameters affecting the polymer preparation and extraction efficiency were evaluated. Morphological characterisation of the MIP and its proper comparison with C18 as a sorbent in solid phase extraction were performed. The optimal conditions for the molecularly imprinted solid phase extraction (MISPE) consisted of 400 μL of sample mixed with 30 mg of MIP and 600 μL of water to reach the optimum solution loading. The loading was followed by a washing step consisting of 1 mL of a 1-propanol solution (1-propanol:water, 30:70,v/v) and elution with 1 mL of 1-propanol. After clean-up, the CoQ10 in the samples was analysed by high performance liquid chromatography. The extraction recoveries were higher than 73.7% with good precision (3.6-8.3%). The limits of detection and quantification were 2.4 and 7.5 μg g(-1), respectively, and a linear range between 7.5 and 150 μg g(-1) of tissue was achieved. The new MISPE procedure provided a successful clean-up for the determination of CoQ10 in a complex matrix.
authors.affiliation: Department of Analytical Chemistry and Physicochemistry, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: Mario
authors.initials: M
authors.lastname: Contin
authors.affiliation: Department of Analytical Chemistry and Physicochemistry, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Flor
authors.affiliation: Department of Analytical Chemistry and Physicochemistry, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: Manuela
authors.initials: M
authors.lastname: Martinefski
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Tecnológicas, CONICET, Buenos Aires, Argentina; Department of Pharmaceutical Technology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: Silvia
authors.initials: S
authors.lastname: Lucangioli
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Tecnológicas, CONICET, Buenos Aires, Argentina; Department of Pharmaceutical Technology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina. Electronic address: vtripodi@ffyb.uba.ar.
authors.forename: Valeria
authors.initials: V
authors.lastname: Tripodi
mesh_terms: D016227:Benzoquinones / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry*; D052616:Solid Phase Extraction; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D016227:Benzoquinones; D011108:Polymers; D012997:Solvents; D014451:Ubiquinone; C109863:ubiquinone-O; C024989:coenzyme Q10
keywords: ATP; BET; Biological sample; Brunauer–Emmett–Teller; CoQ0; CoQ10; Coenzyme Q10; ECD; EGDMA; FEG-SEM; HPLC; IPB; LOD; LOQ; MAA; MIP; MISPE; MS; Molecular imprinting; N; NIP; RSD; SEM; SPE; Solid phase extraction; UC; adenosine-triphosphate; coenzyme Q0; coenzyme Q10; electrochemical detection; ethylene glycoldimethacrylate; field emission gun scanning electron microscopy; high performance liquid chromatography; imprinting-induced promotion of binding; limit of detection; limit of quantification; mass spectrometry; methacrylic acid; molecularly imprinted polymers; molecularly imprinted solid phase extraction; non-imprinted polymer; relative standard deviation; scanning electron microscopy; solid phase extraction; theoretical plates; ubicromenol