title: High-affinity Rb binding, p53 inhibition, subcellular localization, and transformation by wild-type or tumor-derived shortened Merkel cell polyomavirus large T antigens.
abstract: Interference with tumor suppressor pathways by polyomavirus-encoded tumor antigens (T-Ags) can result in transformation. Consequently, it is thought that T-Ags encoded by Merkel cell polyomavirus (MCPyV), a virus integrated in ∼90% of all Merkel cell carcinoma (MCC) cases, are major contributors to tumorigenesis. The MCPyV large T-Ag (LT-Ag) has preserved the key functional domains present in all family members but has also acquired unique regions that flank the LxCxE motif. As these regions may mediate unique functions, or may modulate those shared with T-Ags of other polyomaviruses, functional studies of MCPyV T-Ags are required. Here, we have performed a comparative study of full-length or MCC-derived truncated LT-Ags with regard to their biochemical characteristics, their ability to bind to retinoblastoma (Rb) and p53 proteins, and their transforming potential. We provide evidence that full-length MCPyV LT-Ag may not directly bind to p53 but nevertheless can significantly reduce p53-dependent transcription in reporter assays. Although early region expression constructs harboring either full-length or MCC-derived truncated LT-Ag genes can transform primary baby rat kidney cells, truncated LT-Ags do not bind to p53 or reduce p53-dependent transcription. Interestingly, shortened LT-Ags exhibit a very high binding affinity for Rb, as shown by coimmunoprecipitation and in vitro binding studies. Additionally, we show that truncated MCPyV LT-Ag proteins are expressed at higher levels than those for the wild-type protein and are able to partially relocalize Rb to the cytoplasm, indicating that truncated LT proteins may have gained additional features that distinguish them from the full-length protein.
  
  
  OBJECTIVE
  MCPyV is one of the 12 known polyomaviruses that naturally infect humans. Among these, it is of particular interest since it is the only human polyomavirus known to be involved in tumorigenesis. MCPyV is thought to be causally linked to MCC, a rare skin tumor. In these tumors, viral DNA is monoclonally integrated into the genome of the tumor cells in up to 90% of all MCC cases, and the integrated MCV genomes, furthermore, harbor signature mutations in the so-called early region that selectively abrogate viral replication while preserving cell cycle deregulating functions of the virus. This study describes comparative studies of early region T-Ag protein characteristics, their ability to bind to Rb and p53, and their transforming potential.
authors.affiliation: Institute for Medical Microbiology and Virology, University Medical Center Eppendorf, Hamburg, Germany.
authors.forename: Sophie
authors.initials: S
authors.lastname: Borchert
authors.forename: Manja
authors.initials: M
authors.lastname: Czech-Sioli
authors.forename: Friederike
authors.initials: F
authors.lastname: Neumann
authors.forename: Claudia
authors.initials: C
authors.lastname: Schmidt
authors.forename: Peter
authors.initials: P
authors.lastname: Wimmer
authors.forename: Thomas
authors.initials: T
authors.lastname: Dobner
authors.forename: Adam
authors.initials: A
authors.lastname: Grundhoff
authors.forename: Nicole
authors.initials: N
authors.lastname: Fischer
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D000957:Antigens, Viral, Tumor / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015266:Carcinoma, Merkel Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D045744:Cell Line, Tumor; D002472:Cell Transformation, Viral; D015536:Down-Regulation; D006801:Humans; D007700:Kinetics; D059965:Merkel cell polyomavirus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D027601:Polyomavirus Infections / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D011485:Protein Binding; D021381:Protein Transport; D051381:Rats; D017207:Rats, Sprague-Dawley; D016160:Retinoblastoma Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014412:Tumor Virus Infections / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000821:virology
chemical_list: D000957:Antigens, Viral, Tumor; D016160:Retinoblastoma Protein; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53