title: Evaluation of morpho-anatomical and chemical differences between varieties of the medicinal plant Casearia sylvestris Swartz.
abstract: Casearia sylvestris Swartz (Salicaceae) has been used in traditional medicine and its leaf extracts have been exhibited important pharmacological activities. The species presents morphological, chemical and genetic variation. Two varieties are considered due external morphological differences: C. sylvestris var. sylvestris and var. lingua. There are difficulties in definition of these varieties. The objective of this work is to evaluate chemical and morpho-anatomical differences between C. sylvestris varieties that can be applied in their distinction for pharmaceutical or botanical purposes. Transverse and paradermic sections of leaves were prepared for morpho-anatomical, histochemical and quantitative microscopy (stomatal and palisade index) analyses. Diterpene profiles of the specimens were obtained by HPLC-DAD and TLC. Morpho-anatomical analyses demonstrated significant differences between the varieties only in paradermic sections: var. sylvestris--polygonal epidermic cell walls and hypostomatic; var. lingua--rounded epidermic cell walls and amphistomatic. No differences were observed for stomatal index; palisade index was found 2.8 for var. lingua and 3.9 for var. sylvestris. Chromatographic analyses confirmed previous results demonstrating that diterpene profile in varieties differs, with predominance of these metabolites in var. sylvestris. In conclusion, this work indicates that chromatographic analysis besides morpho-anatomical analysis can be applied in distinction of C. sylvestris varieties.
authors.affiliation: Laboratório de Farmacognosia/LAPDESF, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista/UNESP, Rodovia Araraquara-Jaú, Km 01, Bairro Campus, 14801-902AraraquaraSP, Brasil.
authors.forename: Josiane C
authors.initials: JC
authors.lastname: Claudino
authors.affiliation: Laboratório de Farmacobotânica, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista/UNESP, 14801-902AraraquaraSP, Brasil.
authors.forename: Luis V S do
authors.initials: LV
authors.lastname: Sacramento
authors.affiliation: Curso de Biologia, Universidade Federal de São Carlos/UFSCar, Campus Sorocaba, 18052-780SorocabaSP, Brasil.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Koch
authors.affiliation: Curso de Biologia, Universidade Federal de São Carlos/UFSCar, Campus Sorocaba, 18052-780SorocabaSP, Brasil.
authors.forename: Helen A
authors.initials: HA
authors.lastname: Santos
authors.affiliation: Núcleo de Bioensaios Biossíntese e Ecofisiologia de Produtos Naturais, Instituto de Química, UNESP, 14801-970AraraquaraSP, Brasil.
authors.forename: Alberto J
authors.initials: AJ
authors.lastname: Cavalheiro
authors.affiliation: Instituto Federal de São Paulo, Campus Avançado de Matão, 15990-040MatãoSP, Brasil.
authors.forename: Aristeu G
authors.initials: AG
authors.lastname: Tininis
authors.affiliation: Laboratório de Farmacognosia/LAPDESF, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista/UNESP, Rodovia Araraquara-Jaú, Km 01, Bairro Campus, 14801-902AraraquaraSP, Brasil.
authors.forename: André G dos
authors.initials: AG
authors.lastname: Santos
mesh_terms: D037821:Casearia / Q000033:anatomy & histology* / Q000737:chemistry* / Q000145:classification; D002853:Chromatography, Liquid; D006651:Histocytochemistry; D010936:Plant Extracts / Q000737:chemistry*; D010946:Plants, Medicinal / Q000033:anatomy & histology* / Q000737:chemistry* / Q000145:classification
chemical_list: D010936:Plant Extracts