title: [Genetic variants of the Crimean-Congo hemorrhagic fever virus circulating in endemic areas of the southern Tajikistan in 2009].
abstract: 506 Hyalomma anatolicum ticks were collected and assayed in two Crimean-Congo hemorrhagic fever (CCHF) endemic regions of Tajikistan. Antigen and RNA of CCHF virus were detected in 3.4% of tick pools from Rudaki district using ELISA and RT-PCR tests. As of Tursunzade district, viral antigen was identified in 9.0% of samples and viral RNA was identified in 8.1% of samples. The multiple alignment of the obtained nucleotide sequences of CCHF virus genome S-segment 287-nt region (996-1282) and multiple alignment of deduced amino acid sequences of the samples, carried out to compare with CCHF virus strains from the GenBank database, as well as phylogenetic analysis, enabled us to conclude that Asia 1 and Asia 2 genotypes of CCHF virus are circulating in Tajikistan. It is important to note that the genotype Asia 1 virus was detected for the first time in Tajikistan.
authors.forename: I D
authors.initials: ID
authors.lastname: Petrova
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Kononova
authors.forename: E V
authors.initials: EV
authors.lastname: Chausov
authors.forename: A M
authors.initials: AM
authors.lastname: Shestopalov
authors.forename: F Kh
authors.initials: FKh
authors.lastname: Tishkova
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology; D016679:Genome, Viral; D005838:Genotype; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D006479:Hemorrhagic Fever, Crimean / Q000453:epidemiology / Q000235:genetics / Q000276:immunology / Q000821:virology; D006801:Humans; D026863:Ixodidae / Q000821:virology*; D010802:Phylogeny; D013620:Tajikistan / Q000453:epidemiology
chemical_list: D000956:Antigens, Viral