title: Expression and functional relevance of cannabinoid receptor 1 in Hodgkin lymphoma.
abstract: BACKGROUND
  Cannabinoid receptor 1 (CB1) is expressed in certain types of malignancies. An analysis of CB1 expression and function in Hodgkin lymphoma (HL), one of the most frequent lymphomas, was not performed to date.
  
  
  METHODS
  We examined the distribution of CB1 protein in primary cases of HL. Using lymphoma derived cell lines, the role of CB1 signaling on cell survival was investigated.
  
  
  RESULTS
  A predominant expression of CB1 was found in Hodgkin-Reed-Sternberg cells in a vast majority of classical HL cases. The HL cell lines L428, L540 and KM-H2 showed strong CB1-abundance and displayed a dose-dependent decline of viability under CB1 inhibition with AM251. Further, application of AM251 led to decrease of constitutively active NFκB/p65, a crucial survival factor of HRS-cells, and was followed by elevation of apoptotic markers in HL cells.
  
  
  CONCLUSIONS
  The present study identifies CB1 as a feature of HL, which might serve as a potential selective target in the treatment of Hodgkin lymphoma.
authors.affiliation: Institute of Pathology, Justus-Liebig-University, Giessen, Germany ; Institute of Pathology, Goethe-University, Frankfurt, Germany.
authors.forename: Alexander H
authors.initials: AH
authors.lastname: Benz
authors.affiliation: Institute of Pathology, Goethe-University, Frankfurt, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Renné
authors.affiliation: Institute of Anatomy, Goethe-University, Frankfurt, Germany.
authors.forename: Erik
authors.initials: E
authors.lastname: Maronde
authors.affiliation: Institute of Anatomy, Leipzig University, Leipzig, Germany.
authors.forename: Marco
authors.initials: M
authors.lastname: Koch
authors.affiliation: Department of Anatomy and Cell Biology, Martin-Luther-University Halle-Wittenberg, Halle, Germany.
authors.forename: Urszula
authors.initials: U
authors.lastname: Grabiec
authors.affiliation: Institute of Anatomy, Leipzig University, Leipzig, Germany.
authors.forename: Sonja
authors.initials: S
authors.lastname: Kallendrusch
authors.affiliation: Institute of Pathology, Goethe-University, Frankfurt, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Rengstl
authors.affiliation: Institute of Pathology, Goethe-University, Frankfurt, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Newrzela
authors.affiliation: Institute of Pathology, Goethe-University, Frankfurt, Germany.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Hartmann
authors.affiliation: Institute of Pathology, Goethe-University, Frankfurt, Germany.
authors.forename: Martin-Leo
authors.initials: ML
authors.lastname: Hansmann
authors.affiliation: Institute of Anatomy, Goethe-University, Frankfurt, Germany ; Department of Anatomy and Cell Biology, Martin-Luther-University Halle-Wittenberg, Halle, Germany.
authors.forename: Faramarz
authors.initials: F
authors.lastname: Dehghani
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006689:Hodgkin Disease / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D010880:Piperidines / Q000494:pharmacology*; D011720:Pyrazoles / Q000494:pharmacology*; D043884:Receptor, Cannabinoid, CB1 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism
chemical_list: D010880:Piperidines; D011720:Pyrazoles; D043884:Receptor, Cannabinoid, CB1; D051996:Transcription Factor RelA; C103505:AM 251