title: A nonadaptive origin of a beneficial trait: in silico selection for free energy of folding leads to the neutral emergence of mutational robustness in single domain proteins.
abstract: Proteins are regarded as being robust to the deleterious effects of mutations. Here, the neutral emergence of mutational robustness in a population of single domain proteins is explored using computer simulations. A pairwise contact model was used to calculate the ΔG of folding (ΔG folding) using the three dimensional protein structure of leech eglin C. A random amino acid sequence with low mutational robustness, defined as the average ΔΔG resulting from a point mutation (ΔΔG average), was threaded onto the structure. A population of 1,000 threaded sequences was evolved under selection for stability, using an upper and lower energy threshold. Under these conditions, mutational robustness increased over time in the most common sequence in the population. In contrast, when the wild type sequence was used it did not show an increase in robustness. This implies that the emergence of mutational robustness is sequence specific and that wild type sequences may be close to maximal robustness. In addition, an inverse relationship between ∆∆G average and protein stability is shown, resulting partly from a larger average effect of point mutations in more stable proteins. The emergence of mutational robustness was also observed in the Escherichia coli colE1 Rop and human CD59 proteins, implying that the property may be common in single domain proteins under certain simulation conditions. The results indicate that at least a portion of mutational robustness in small globular proteins might have arisen by a process of neutral emergence, and could be an example of a beneficial trait that has not been directly selected for, termed a "pseudaptation."
authors.affiliation: Physics Department, University of Puerto Rico - Rio Piedras, San Juan, PR, USA.
authors.forename: Rafael F
authors.initials: RF
authors.lastname: Pagan
authors.forename: Steven E
authors.initials: SE
authors.lastname: Massey
mesh_terms: D000220:Adaptation, Biological; D019143:Evolution, Molecular; D008962:Models, Theoretical; D009154:Mutation; D017510:Protein Folding; D055550:Protein Stability; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D019655:Quantitative Trait, Heritable; D012641:Selection, Genetic
chemical_list: D011506:Proteins