title: Isolation of atypical genotype Actinobacillus pleuropneumoniae serotype 6 in Japan.
abstract: We describe here isolation of genetically atypical serotype 6 Actinobacillus pleuropneumoniae in Japan indistinguishable by the multiplex PCR that can discriminate between immunologically cross-reactive serotypes 3, 6 and 8. Nucleotide sequence analysis of capsular export and biosynthesis genes revealed that the atypical isolates have capsular polysaccharide export and synthesis gene sequences that are distinct from those of the serotype 6 reference strain. The atypical strains contain a sequence that is identical with both serotype 3- and 6-specific primers, which causes cross-reactions in multiplex PCR.
authors.affiliation: National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Hiroya
authors.initials: H
authors.lastname: Ito
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Katsuragi
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Akama
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yuzawa
mesh_terms: D000189:Actinobacillus Infections / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005838:Genotype; D007564:Japan; D008969:Molecular Sequence Data; D060885:Multiplex Polymerase Chain Reaction / Q000662:veterinary; D011135:Polysaccharides, Bacterial / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000662:veterinary; D065288:Serogroup; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: D017931:DNA Primers; D011135:Polysaccharides, Bacterial; D011994:Recombinant Proteins