title: Novel in-situ longitudinal model for the study of dentifrices on dental erosion-abrasion.
abstract: A novel longitudinal erosion-abrasion in-situ model was proposed. In an exploratory test (phase 1) toothbrushing effect was investigated using a parallel design, whereas in the main study (phases 1 + 2), a crossover design tested the effect of fluoride dentifrice. In phase 1, 16 subjects (n = 5-6 subjects per group) wore partial dentures with enamel specimens for 28 d and adhered to one of the following treatment regimens: regimen A, erosion only; regimen B, erosion + toothbrushing with fluoride-containing dentifrice [1,100 ppm of fluoride as sodium fluoride (NaF)]; and regimen C, erosion + toothbrushing with placebo dentifrice (0 ppm fluoride). Erosion consisted of the exposure of specimens to grapefruit juice. In regimens B and C, toothbrushing was performed with the test dentifrices 5 min after erosion. For the main study a second phase was carried out in which subjects initially treated with regimen B were treated with regimen C, and vice versa (crossover, n = 11), repeating the same experimental protocol. Enamel wear was measured by optical profilometry at baseline and every 7 d thereafter. In the exploratory test (phase 1), no significant differences were observed among regimens at any of the study time-points. In the main study (phases 1 + 2), higher enamel wear was observed for regimen C than for regimen B. A significant trend was observed for the increase of enamel wear over time. Fluoridated dentifrice reduced the development of erosive-abrasive lesions. The proposed longitudinal model provided adequate responses for erosion-abrasion frequency and fluoride effects.
authors.affiliation: Department of Preventive and Community Dentistry, Oral Health Research Institute, Indiana University School of Dentistry, Indianapolis, IN, USA.
authors.forename: Anderson T
authors.initials: AT
authors.lastname: Hara
authors.forename: Ashley P
authors.initials: AP
authors.lastname: Barlow
authors.forename: George J
authors.initials: GJ
authors.lastname: Eckert
authors.forename: Domenick T
authors.initials: DT
authors.lastname: Zero
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001628:Beverages / Q000009:adverse effects; D002327:Cariostatic Agents / Q000627:therapeutic use; D032083:Citrus paradisi; D018592:Cross-Over Studies; D003743:Dental Enamel / Q000187:drug effects; D003802:Dentifrices / Q000627:therapeutic use*; D003804:Dentin / Q000187:drug effects; D005260:Female; D005500:Follow-Up Studies; D005638:Fruit; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D010919:Placebos; D016037:Single-Blind Method; D012969:Sodium Fluoride / Q000627:therapeutic use; D014072:Tooth Abrasion / Q000209:etiology* / Q000517:prevention & control; D014077:Tooth Erosion / Q000209:etiology* / Q000517:prevention & control; D014099:Toothbrushing / Q000379:methods
chemical_list: D002327:Cariostatic Agents; D003802:Dentifrices; D010919:Placebos; D012969:Sodium Fluoride
keywords: enamel; fluoride; profilometry