title: Diphenyleneiodonium, an inhibitor of NOXes and DUOXes, is also an iodide-specific transporter.
abstract: NADPH oxidases (NOXes) and dual oxidases (DUOXes) generate O2 (.-) and H2O2. Diphenyleneiodonium (DPI) inhibits the activity of these enzymes and is often used as a specific inhibitor. It is shown here that DPI, at concentrations similar to those which inhibit the generation of O2 derivatives, activated the efflux of radioiodide but not of its analog (99m)TcO4 (-) nor of the K(+) cation mimic (86)Rb(+) in thyroid cells, in the PCCl3 rat thyroid cell line and in COS cell lines expressing the iodide transporter NIS. Effects obtained with DPI, especially in thyroid cells, should therefore be interpreted with caution.
authors.affiliation: IRIBHM, School of Medicine, ULB, Université Libre de Bruxelles, Campus Erasme, Route de Lennik 808, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Massart
authors.affiliation: IRIBHM, School of Medicine, ULB, Université Libre de Bruxelles, Campus Erasme, Route de Lennik 808, Belgium.
authors.forename: N
authors.initials: N
authors.lastname: Giusti
authors.affiliation: Laboratory of Physiology, School of Medicine, ULB, Université Libre de Bruxelles, Campus Erasme, Route de Lennik 808, Belgium.
authors.forename: R
authors.initials: R
authors.lastname: Beauwens
authors.affiliation: IRIBHM, School of Medicine, ULB, Université Libre de Bruxelles, Campus Erasme, Route de Lennik 808, Belgium.
authors.forename: J E
authors.initials: JE
authors.lastname: Dumont
authors.affiliation: IRIBHM, School of Medicine, ULB, Université Libre de Bruxelles, Campus Erasme, Route de Lennik 808, Belgium.
authors.forename: F
authors.initials: F
authors.lastname: Miot
authors.affiliation: IRIBHM, School of Medicine, ULB, Université Libre de Bruxelles, Campus Erasme, Route de Lennik 808, Belgium.
authors.forename: J Van
authors.initials: JV
authors.lastname: Sande
keywords: DPI, diphenyleneiodonium; DUOX; DUOXes, dual oxidases; Diphenyleneiodonium; HRP, horse radish peroxidase type II; HVA, homovanillic acid; Iodide efflux; KRH, Krebs–Ringer Hepes medium; MMI, methylmercaptoimidazole; NIS, sodium/iodide symporter; NOX; NOXes, NADPH oxidases; O2 radical generation; Thyroid