title: Human platelets express functional thymic stromal lymphopoietin receptors: a potential role in platelet activation in acute coronary syndrome.
abstract: BACKGROUND
  Thymic stromal lymphopoietin (TSLP) has been shown to be expressed in various inflammatory tissues, such as human atherosclerotic plaques. Many types of myeloid cells involved in atherosclerosis, including mast cells, lymphocytes, dendritic cells and monocytes/macrophages, present TSLP receptors (TSLPR). However, it is unknown whether platelets, which also play important roles in atherothrombosis, express TSLPR.
  
  
  RESULTS
  We applied flow cytometry and western blotting to show that TSLPR was expressed on the surface of human platelets. Following the addition of TSLP to platelets, the expression of CD62P, CD63, PAC-1 and p-Akt as well as aggregation and ATP release were increased significantly. A TSLPR antibody and a PI3K (phosphatidylinositol 3-kinase) enzyme inhibitor (LY294002) significantly inhibited the platelet activation induced by TSLP. The expression of TSLPR, CD62P and CD63 and the increment of the expression of CD62P and CD63 induced by TSLP in the acute coronary syndrome (ACS) group were markedly higher than those in the control group and the stable angina pectoris (SAP) group. The expression and the increment of the expression of CD62P and CD63 induced by TSLP were positively correlated with the expression of TSLPR.
  
  
  CONCLUSIONS
  Human platelets express functional TSLPR, which can be activated by TSLP to promote platelet activation. TSLP/TSLPR functions via activating the PI3K/AKT pathway, and this signalling pathway may be one of the mechanisms involved in thrombosis in ACS. In coronary disease patients, the determination of TSLPR in platelets may help to identify the risk of ACS.
authors.affiliation: Department of Cardiology, Institute of Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Boyuan
authors.initials: B
authors.lastname: Wang
authors.forename: Yudong
authors.initials: Y
authors.lastname: Peng
authors.forename: Jiangchuan
authors.initials: J
authors.lastname: Dong
authors.forename: Jing
authors.initials: J
authors.lastname: Lin
authors.forename: Chun
authors.initials: C
authors.lastname: Wu
authors.forename: Yousu
authors.initials: Y
authors.lastname: Su
authors.forename: Hongcheng
authors.initials: H
authors.lastname: Fang
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Kai
authors.initials: K
authors.lastname: Huang
authors.forename: Dazhu
authors.initials: D
authors.lastname: Li
mesh_terms: D054058:Acute Coronary Syndrome / Q000378:metabolism* / Q000473:pathology; D000368:Aged; D000787:Angina Pectoris / Q000378:metabolism / Q000473:pathology; D001792:Blood Platelets / Q000378:metabolism*; D002867:Chromones / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009025:Morpholines / Q000494:pharmacology; D019007:P-Selectin / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D000081082:Phosphoinositide-3 Kinase Inhibitors; D010766:Phosphorylation / Q000187:drug effects; D015539:Platelet Activation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D018121:Receptors, Cytokine / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D060149:Tetraspanin 30 / Q000378:metabolism; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D002867:Chromones; D016207:Cytokines; D004791:Enzyme Inhibitors; D009025:Morpholines; D019007:P-Selectin; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D051057:Proto-Oncogene Proteins c-akt; D018121:Receptors, Cytokine; D060149:Tetraspanin 30; D000094632:Thymic Stromal Lymphopoietin; C498762:CRLF2 protein, human; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one