title: Location and architecture of an antibody-binding site of influenza A virus nucleoprotein.
abstract: Amino acid positions recognized by monoclonal antibodies (MAbs) in the influenza A virus nucleoprotein (NP) have been reported. As these residues were scattered in the three-dimensional (3D) structure of NP, no patterns of the architecture of antibody-binding sites could be inferred. Here, we used site-specific mutagenesis and ELISA to screen the amino acids surrounding position 470 recognized by the MAb 3/1 as a linear epitope. Ten amino acid residues involved in the reaction of NP with the MAb 3/1 and the MAb 469/6 were identified. Our data are the first to outline a compact site recognized by MAbs in the 3D structure of the influenza virus NP.
authors.affiliation: D. I. Ivanovsky Institute of Virology, Gamaleya Str. 16, 123098, Moscow, Russia, nataliavarich@rambler.ru.
authors.forename: Natalia L
authors.initials: NL
authors.lastname: Varich
authors.forename: Galina K
authors.initials: GK
authors.lastname: Sadykova
authors.forename: Alexei G
authors.initials: AG
authors.lastname: Prilipov
authors.forename: Konstantin S
authors.initials: KS
authors.lastname: Kochergin-Nikitsky
authors.forename: Robert G
authors.initials: RG
authors.lastname: Webster
authors.forename: Nikolai V
authors.initials: NV
authors.lastname: Kaverin
mesh_terms: D001666:Binding Sites, Antibody / Q000235:genetics*; D004252:DNA Mutational Analysis; D009980:Influenza A virus / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D019590:Nucleocapsid Proteins; D011487:Protein Conformation; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D014758:Viral Core Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*
chemical_list: C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins