title: [Microfluidic evaporative light scattering detector coupled with capillary liquid chromatography and its application to Ginkgo biloba extract analysis].
abstract: A novel separation system of microfluidic evaporative light scattering detector (microELSD) coupled with capillary liquid chromatography (cLC) was built and applied to the separation and detection of herbal medicine Ginkgo biloba extract and its disperse tablet formulation. Compared with the traditional HPLC, this microELSD-cLC system consumed much less sample and solvent. Some key parameters were optimized. It was found that the higher the evaporization temperature of the drift tube, the higher S/N could be achieved. The mobile phase A was 0.05% (v/v) trifluoroacetic acid (TFA), and the mobile phase B was methanol containing 0.05% (v/v) TFA. The optimized gradient conditions were as follows: 0-10 min, 5% B - 25% B; 10-25 min, 25% B - 38% B; 25-35 min, 38% B; 35-40 min, 38% B - 42% B; 40-55 min, 42% B - 50% B. The complex herbal medicine Ginkgo biloba extract and its disperse tablet formulation were successfully separated. Four main therapeutic components (bilobalide and ginkgolide A, B, C) were finally separated and determined, which were terpene lactones with pretty weak UV absorption. The RSDs of the detected terpene lactones from different manufacturers were all no more than 2.42%, which proved this platform's good analysis repeatability. The results showed the applicability of the platform to the analysis of complex traditional Chinese medicines.
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Gu
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yan
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003517:Cyclopentanes / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D005663:Furans / Q000032:analysis; D020441:Ginkgo biloba / Q000737:chemistry*; D046934:Ginkgolides / Q000032:analysis; D007783:Lactones / Q000032:analysis; D044085:Microfluidics; D010936:Plant Extracts / Q000032:analysis*; D012542:Scattering, Radiation; D013729:Terpenes / Q000032:analysis
chemical_list: D003517:Cyclopentanes; D004365:Drugs, Chinese Herbal; D005663:Furans; D046934:Ginkgolides; D007783:Lactones; D010936:Plant Extracts; D013729:Terpenes; C073710:bilobalide; C477042:ginkgolide A