title: [Association between GSTP1, GSTM1, GSTT1 genetic polymorphisms and urinary styrene phenyl hydroxyethyl mercapturic acids level].
abstract: OBJECTIVE
  To investigate the relationship between genetic polymorphisms of glutathione S-transferase P1 (GSTP1), glutathione S-transferase M1 (GSTM1), and glutathione S-transferase T1 (GSTT1) and urinary level of mercapturic acids of styrene (PHEMAs) in workers exposed to styrene.
  
  
  METHODS
  One hundred and twenty-six workers exposed to styrene were selected as exposure group, and 150 workers without styrene exposure as the control group; all the workers came from a locomotive shell production factory in Shandong Province, China. The PCR-RFLP technique was applied to analyze the individual genetic polymorphisms of GSTP1; the multiplex PCR technique was used to investigate the genetic polymorphisms of GSTM1 and GSTT1; the relationship between genetic polymorphisms of GSTP1, GSTM1, and GSTT1 and urinary level of PHEMAs in workers exposed to styrene was statistically analyzed.
  
  
  RESULTS
  The three genotypes investigated in the study had a distribution in accordance with the Chinese population. With exposure to high- concentration styrene, the individuals carrying GSTP1 (exon5, A105G) AA genotype (wildtype) had a significantly higher urinary level of PHEMAs (43.58 mg/g) than those with mutant genotypes AG (29.769 mg/g) and GG (30.245 mg/g); the urinary level of PHEMAs in individuals carrying wild-type GSTM1 genotype was significantly higher than that in individuals carrying deficient-type GSTM1 genotype (40.197 mg/g vs 28.866 mg/g, P < 0.05); no significant difference in urinary level of PHEMAs was found between individuals carrying wild-type GSTT1 genotype and deficient-type GSTT1 genotype. There was no significant relationship between the three gene polymorphisms and urinary level of PHEMAs in the control group.
  
  
  CONCLUSIONS
  The genetic polymorphisms of GSTP1 and GSTM1 may be related to urinary level of PHEMAs in workers exposed to styrene.
authors.affiliation: Shandong Academy of Occupational Health and Occupational Medicine, jinan 250062, China.
authors.forename: Wei-nan
authors.initials: WN
authors.lastname: Han
authors.affiliation: Shandong Academy of Occupational Health and Occupational Medicine, jinan 250062, China. E-mail: china.shaohua@yahoo.com.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Shao
authors.affiliation: Shandong Academy of Occupational Health and Occupational Medicine, jinan 250062, China.
authors.forename: Xue-lei
authors.initials: XL
authors.lastname: Chen
authors.affiliation: Shandong Academy of Occupational Health and Occupational Medicine, jinan 250062, China.
authors.forename: Gui-zhi
authors.initials: GZ
authors.lastname: Han
authors.affiliation: Shandong Academy of Occupational Health and Occupational Medicine, jinan 250062, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Meng
mesh_terms: D000111:Acetylcysteine / Q000652:urine*; D000328:Adult; D005260:Female; D005838:Genotype; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D016273:Occupational Exposure; D011110:Polymorphism, Genetic; D020058:Styrene / Q000652:urine*; D055815:Young Adult
chemical_list: D000111:Acetylcysteine; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D020058:Styrene; C413545:glutathione S-transferase T1; C496556:GSTP1 protein, human