title: Antiproliferative effect of flavonoids from the halophyte Vitex rotundifolia on human cancer cells.
abstract: Three flavonoids, luteolin (1), vitexicarpin (2) and artemetin (3), from the salt marsh plant Vitex rotundifolia, were tested for their anti-proliferative activities in AGS, MCF-7 and HT-29 human cancer cell lines and compared with the control using MTT assay. Among them, 2 was most effective with an IC50 of 6.9 and 22.8 microM against AGS and HT-29 cells, respectively. Inaddition, mRNA expression levels of major apoptosis-related genes such as p21, p53, Bcl-2 andBax in AGS cells were evaluated by reverse-transcription polymerase chain reaction (RT-PCR). Compound 2 not only enhanced most remarkably the expression level of tumor suppressor genes p53 and p21, and pro-apoptotic gene Bax at a concentration of 25 microM, but also suppressed the expression level of antiapoptotic gene Bcl-2 to 20% at the same concentration, thus shifting the Bax/Bcl-2 ratio in favor of apoptosis.
authors.affiliation: Bio Materials R&D Center, Kolmar Korea Co., Ltd. CBTP, Future Convergent Tech Center, Room 406, 685-3, Yangcheong-ri, Ochang-eup, Cheongwon-gun, Chungbuk, 619-705, Republic of Korea.
authors.forename: You Ah
authors.initials: YA
authors.lastname: Kim
authors.affiliation: Division of Marine Environment and Bioscience, Korea Maritime University, Busan 606-791, Republic of Korea.
authors.forename: Hojun
authors.initials: H
authors.lastname: Kim
authors.affiliation: Division of Marine Environment and Bioscience, Korea Maritime University, Busan 606-791, Republic of Korea.
authors.forename: Youngwan
authors.initials: Y
authors.lastname: Seo
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005419:Flavonoids / Q000302:isolation & purification* / Q000494:pharmacology; D019073:HT29 Cells; D006801:Humans; D061986:MCF-7 Cells; D010946:Plants, Medicinal / Q000737:chemistry; D055051:Salt-Tolerant Plants / Q000737:chemistry; D020950:Vitex / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids