title: Undibacterium macrobrachii sp. nov., isolated from a freshwater shrimp culture pond.
abstract: A bacterial strain designated CMJ-9(T) was isolated from a freshwater shrimp culture pond in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain CMJ-9(T) were strictly aerobic, Gram-negative, motile by a single polar flagellum, poly-β-hydroxybutyrate-containing and formed light-yellow colonies. Growth occurred at 10-37 °C (optimum, 20-30 °C), with 0-0.8 % NaCl (optimum, 0-0.1 %) and at pH 6.0-9.0 (optimum, pH 6.0-7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CMJ-9(T) belonged to the genus Undibacterium, and its closest neighbour was Undibacterium seohonense SHS5-24(T), with 96.7 % sequence similarity. The predominant cellular fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The major cellular hydroxy fatty acid was C10 : 0 3-OH. The polar lipid profile consisted of the predominant lipids phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The polyamine profile was composed of the major compound putrescine and moderate amounts of 2-hydroxyputrescine. The major respiratory quinone was Q-8 and the DNA G+C content was 47.7 mol%. On the basis of the phylogenetic and phenotypic data, strain CMJ-9(T) should be classified within a novel species, for which the name Undibacterium macrobrachii sp. nov. is proposed. The type strain is CMJ-9(T) ( = BCRC 80406(T) = LMG 26891(T) = KCTC 23916(T)).
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Shih-Yi
authors.initials: SY
authors.lastname: Sheu
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Yang-Shun
authors.initials: YS
authors.lastname: Lin
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Jhen-Ci
authors.initials: JC
authors.lastname: Chen
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
mesh_terms: D000818:Animals; D017756:Aquaculture; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D033364:Decapoda; D005227:Fatty Acids / Q000737:chemistry; D006885:Hydroxybutyrates / Q000737:chemistry; D008969:Molecular Sequence Data; D042802:Oxalobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D011073:Polyamines / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry; D061005:Ponds / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013624:Taiwan; D014451:Ubiquinone / Q000737:chemistry; D000094122:Polyhydroxybutyrates
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006885:Hydroxybutyrates; D011073:Polyamines; D011091:Polyesters; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C030778:ubiquinone 8