title: Cryogenic grinding of electrospun poly-ε-caprolactone mesh submerged in liquid media.
abstract: In this paper, the treatment of poly-ε-caprolactone (PCL) nano/micro-mesh system by cryogenic grinding and subsequent characterization of obtained product is described. The PCL nano/micro-mesh layer submerged in appropriate liquid was cryogenically ground and obtained particles were characterized employing mainly laser diffraction and scanning electron microscopy (SEM). In the ground sample, different types of particles (fibrous particles, fibrous fragments, agglomerates with and without an internal fibrous structure, lamellae and nanoparticles) were identified, described and quantified. Parameters of cryogenic grinding (weight of sample, type of liquid medium, and influence of sample storage) were optimized to maximize the yield of particles with desired features. The potential of the system for cell scaffolding was demonstrated by cultivation of 3T3 fibroblasts on the produced microparticles.
authors.affiliation: University of Pardubice, Faculty of Chemical Technology, Studentská 573, 532 10 Pardubice, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Knotek
authors.affiliation: University of Pardubice, Institute of Environmental and Chemical Engineering, Studentská 573, 530 12 Pardubice, Czech Republic.
authors.forename: Miloslav
authors.initials: M
authors.lastname: Pouzar
authors.affiliation: Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, V Úvalu 84, 150 06, Prague 5, Czech Republic; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, v.v.i, Vídeňská 1083,142 20, Prague 4, Czech Republic.
authors.forename: Matej
authors.initials: M
authors.lastname: Buzgo
authors.affiliation: University of Pardubice, Department of Biological and Biochemical Sciences, Studentská 573, 530 12 Pardubice, Czech Republic.
authors.forename: Barbora
authors.initials: B
authors.lastname: Krizkova
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of Czech Republic, v.v.i., Heyrovskeho sq. 2, 162 06 Prague, Czech Republic.
authors.forename: Milan
authors.initials: M
authors.lastname: Vlcek
authors.affiliation: Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, V Úvalu 84, 150 06, Prague 5, Czech Republic; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, v.v.i, Vídeňská 1083,142 20, Prague 4, Czech Republic.
authors.forename: Andrea
authors.initials: A
authors.lastname: Mickova
authors.affiliation: Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, V Úvalu 84, 150 06, Prague 5, Czech Republic; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, v.v.i, Vídeňská 1083,142 20, Prague 4, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Plencner
authors.affiliation: University of Pardubice, Institute of Environmental and Chemical Engineering, Studentská 573, 530 12 Pardubice, Czech Republic.
authors.forename: Jakub
authors.initials: J
authors.lastname: Navesnik
authors.affiliation: Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, V Úvalu 84, 150 06, Prague 5, Czech Republic; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, v.v.i, Vídeňská 1083,142 20, Prague 4, Czech Republic.
authors.forename: Evzen
authors.initials: E
authors.lastname: Amler
authors.affiliation: University of Pardubice, Department of Inorganic Technology, Studentská 573, 530 12 Pardubice, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Belina
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D011091:Polyesters; C016240:polycaprolactone