title: Distribution of MLH1 foci in horse male synaptonemal complex.
abstract: Advances in molecular cytogenetics have provided the opportunity to study events during prophase I of meiosis. Immunofluorescent localization of different meiotic protein components were used to characterize the early stages of the first meiotic division in horse spermatocytes. The frequency and distribution of recombination events during prophase I were investigated using the mutL homolog 1 (MLH1) protein that is known to be associated with these events. The frequency and distribution of MLH1 foci were investigated in pachytene nuclei of 6 fertile stallions, and the average relative synaptonemal complex length was found to be highly correlated with the average number of MLH1 foci. The frequency and distribution of MLH1 foci were found to closely correspond to the frequency and distribution of chiasmata on metaphase I chromosomes, and genetic length, calculated from MLH1 foci data, for the whole genome was 2,505.5 cM.
authors.affiliation: Molecular Biology and Genetic, Central Veterinary Research Laboratory, Dubai, UAE.
authors.forename: A
authors.initials: A
authors.lastname: Al-Jaru
authors.forename: W
authors.initials: W
authors.lastname: Goodwin
authors.forename: J
authors.initials: J
authors.lastname: Skidmore
authors.forename: K
authors.initials: K
authors.lastname: Khazanehdari
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D045643:DNA Repair Enzymes / Q000235:genetics; D005787:Gene Frequency; D006736:Horses / Q000235:genetics / Q000378:metabolism*; D008297:Male; D049469:Meiotic Prophase I / Q000235:genetics*; D008854:Microscopy, Electron / Q000662:veterinary; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D013090:Spermatocytes / Q000166:cytology; D013091:Spermatogenesis / Q000235:genetics; D013573:Synaptonemal Complex / Q000235:genetics / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D009687:Nuclear Proteins; D045643:DNA Repair Enzymes