title: [blaTEM, blaSHV y blaCTX-M genes in extended-spectrum beta-lactamases producing enterobacterias isolated from patients with hospital-acquired infections].
abstract: The objective of the present investigation was to identify the blaTEM, blaSHV, and blaCTX-M genes on extended-spectrum beta-lactamases (ESBL) producing Enterobacteriaceae from clinical isolates, collected between September and November 2005. In addition to third-generation cephalosporin resistance, the isolates also showed resistance to chloramphenicol (59.2%), amikacin (37.0%) and gentamicin (40.7%), and demonstrated sensitivity to imipenem and meropenem. Nine strains were capable of transferring third-generation cephalosporin resistance, as well as the production of ESBL. In the clinical isolates, the genes blaSHV, blaTEM and blaCTX-M were detected, being more prevalent the types blaTEM-1, blaSHV-1, blaSHV-5 blaSHV-5-2a and blaCTX-M-1; while in the trans-conjugated only blaTEM-1, blaSHV-5 y blaSHV-5-2awere found. In total, seven types of genes were identified, five of which were codifying genes for ESBL-type enzymes. This demonstrates that in the hospital center, resistance to third-generation cephalosporin is mediated by several enzymes.
authors.affiliation: Laboratorio de Bacteriología Molecular, Departamento de Bioanálisis, Universidad de Oriente, Cumaná, estado Sucre. miltzaguz@yahoo.es
authors.forename: Militza
authors.initials: M
authors.lastname: Guzmán
authors.affiliation: Laboratorio de Bacteriología Molecular, Departamento de Bioanálisis, Universidad de Oriente, Cumaná, estado Sucre.
authors.forename: Eliosmar
authors.initials: E
authors.lastname: Rodríguez
authors.affiliation: Laboratorio de Bacteriología Molecular, Departamento de Bioanálisis, Universidad de Oriente, Cumaná, estado Sucre.
authors.forename: Karen
authors.initials: K
authors.lastname: Antón
authors.affiliation: Laboratorio de Bacteriología Molecular, Departamento de Bioanálisis, Universidad de Oriente, Cumaná, estado Sucre.
authors.forename: Suyin
authors.initials: S
authors.lastname: Silva
authors.affiliation: Laboratorio de Bacteriología Molecular, Departamento de Bioanálisis, Universidad de Oriente, Cumaná, estado Sucre.
authors.forename: Jhonilys
authors.initials: J
authors.lastname: Navarro
authors.affiliation: Laboratorio de Bacteriología Molecular, Departamento de Bioanálisis, Universidad de Oriente, Cumaná, estado Sucre.
authors.forename: Loriannys
authors.initials: L
authors.lastname: Lastra
authors.affiliation: Laboratorio de Bacteriología Molecular, Departamento de Bioanálisis, Universidad de Oriente, Cumaná, estado Sucre.
authors.forename: Elsa
authors.initials: E
authors.lastname: Salazar
authors.affiliation: Laboratorio de Biología de Plásmidos, Instituto de Biología Experimental, Universidad Central de Venezuela, Caracas, Venezuela.
authors.forename: Guillermina
authors.initials: G
authors.lastname: Alonso
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D003428:Cross Infection / Q000235:genetics / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004754:Enterobacter / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000235:genetics / Q000382:microbiology*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D013379:Substrate Specificity; D001618:beta-Lactamases / Q000235:genetics* / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D001618:beta-Lactamases