title: Ovarian minimal residual disease in chronic myeloid leukaemia.
abstract: The options for fertility preservation include cryopreservation of ovarian tissue. Although transplantation of cryopreserved-thawed ovarian tissue in cancer survivors has resulted in live births, there is evidence of malignancy involvement in ovarian tissue, especially in leukaemia. The objectives of this study were to investigate the involvement of chronic myeloid leukaemia (CML) in ovaries by both pathological/immunohistochemical methods and PCR for the identification of the Philadelphia chromosome (BCR-ABL transcripts). The patient was a survivor of paediatric CML whose ovaries were cryopreserved. The patient became infertile and requested ovarian reimplantation in adulthood. Pathological examinations of ovarian tissue with immunohistochemical stainings, quantitative PCR and two-step nested PCR were applied to identify BCR-ABL transcripts. Despite the lack of positive pathological/immunohistochemical evidence, PCR and two-step nested PCR revealed that the ovary was contaminated by malignant minimal residual CML. Survivors of childhood CML may harbour minimal residual disease in the ovaries. This finding stresses the danger of reseeding cancer by ovarian grafting, especially in patients with leukaemia. If ovarian grafting is considered, reimplantation should be preceded by examination of ovarian samples both pathologically and by molecular techniques. On the basis of molecular findings, ovarian autografting was not recommended in this case report.
authors.affiliation: Infertility and IVF Unit, Beilinson Women Hospital, Rabin Medical Center, Petach Tikva, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel. Electronic address: ronita@clalit.org.il.
authors.forename: Ronit
authors.initials: R
authors.lastname: Abir
authors.affiliation: Hematology Laboratory, Rabin Medical Center, Petah Tikva, Israel; Hemato-Oncology Laboratory, Felsenstein Medical Research Center, Tel Aviv University, Petah Tikva, Israel.
authors.forename: Adina
authors.initials: A
authors.lastname: Aviram
authors.affiliation: Department of Pathology, Rabin Medical Center, Beilinson Hospital, Petah Tikva, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Meora
authors.initials: M
authors.lastname: Feinmesser
authors.affiliation: Department of Pediatric Hematology Oncology, Schneider Children's Medical Center of Israel, Petah Tikva, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Jerry
authors.initials: J
authors.lastname: Stein
authors.affiliation: Department of Pediatric Hematology Oncology, Schneider Children's Medical Center of Israel, Petah Tikva, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Isaac
authors.initials: I
authors.lastname: Yaniv
authors.affiliation: Hematology Laboratory, Rabin Medical Center, Petah Tikva, Israel; Hemato-Oncology Laboratory, Felsenstein Medical Research Center, Tel Aviv University, Petah Tikva, Israel.
authors.forename: Doris
authors.initials: D
authors.lastname: Parnes
authors.affiliation: Infertility and IVF Unit, Beilinson Women Hospital, Rabin Medical Center, Petach Tikva, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Avi
authors.initials: A
authors.lastname: Ben-Haroush
authors.affiliation: IVF Unit, Division of Obstetrics and Gynecology, Sheba Medical Center, Tel Hashomer, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Dror
authors.initials: D
authors.lastname: Meirow
authors.affiliation: Hematology Laboratory, Rabin Medical Center, Petah Tikva, Israel; Hemato-Oncology Laboratory, Felsenstein Medical Research Center, Tel Aviv University, Petah Tikva, Israel.
authors.forename: Esther
authors.initials: E
authors.lastname: Rabizadeh
authors.affiliation: Infertility and IVF Unit, Beilinson Women Hospital, Rabin Medical Center, Petach Tikva, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Fisch
mesh_terms: D015925:Cryopreservation; D005260:Female; D016313:Genes, abl / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D007951:Leukemia, Myeloid / Q000473:pathology*; D010053:Ovary / Q000033:anatomy & histology / Q000473:pathology* / Q000637:transplantation; D010677:Philadelphia Chromosome; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017741:Survivors
keywords: PCR; Philadelphia chromosome; cancer seeding by ovarian grafting; chronic myeloid leukaemia; ovarian transplantation; pathological/immunohistochemical examination