title: Preparative isolation and purification of five flavonoid glycosides and one benzophenone galloyl glycoside from Psidium guajava by high-speed counter-current chromatography (HSCCC).
abstract: Psidium guajava leaves have a diverse phytochemical composition including flavonoids, phenolics, meroterpenoids and triterpenes, responsible for the biological activities of the medicinal parts. In particular, flavonol glycosides show beneficial effects on type II diabetes mellitus. A simple and efficient HSCCC method has been developed for the preparative separation of five flavonoid glycosides and one diphenylmethane glycoside from P. guajava. A solvent system composed of n-hexane-ethyl acetate-methanol-water (0.7:4:0.8:4, v/v/v/v) was optimized for the separation. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase. Under the optimized conditions, hyperoside (15.3 mg), isoquercitrin (21.1 mg), reynoutrin (65.2 mg), quercetin-3-O-β-D-arabinopyranoside (71.7 mg), quercetin-3-O-α-L-arabinofuranoside (105.6 mg) and 2,4,6-trihydroxy-3,5-dimethylbenzophenone 4-O-(6''-O-galloyl)-β-D-glucopyranoside (98.4 mg) were separated from crude sample (19.8 g). The structures of all the isolates were identified by ESI-MS, 1H- and 13C-NMR analyses and their purities (>95%) were determined using HPLC.
authors.forename: Yindi
authors.initials: Y
authors.lastname: Zhu
authors.forename: Yue
authors.initials: Y
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhan
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Traditional Chinese Medicine Chemistry, School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, No. 6 Wangjing Zhonghuan South Road, Chaoyang District, Beijing 100102, China. thxu@163.com.
authors.forename: Tunhai
authors.initials: T
authors.lastname: Xu
authors.forename: Tonghua
authors.initials: T
authors.lastname: Liu
mesh_terms: D001577:Benzophenones / Q000737:chemistry*; D003377:Countercurrent Distribution; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification*; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification*; D015394:Molecular Structure; D031647:Psidium / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: D001577:Benzophenones; D005419:Flavonoids; D006027:Glycosides; D012997:Solvents; C047723:benzophenone