title: Purification of VDAC1 from rat liver mitochondria.
abstract: To make biophysical measurements of functions such as the pore-forming activity of mitochondrial voltage-dependent anion-selective channel protein 1 (VDAC1), it is first necessary to obtain a source of purified VDAC protein. In this protocol, we present a method for obtaining rat liver mitochondria as a source of VDAC1 and then describe two methods, one using a nonionic detergent and the other an ionic detergent, for purifying VDAC1 from the isolated mitochondria. This produces a source of VDAC1 proteins that are suitable for subsequent incorporation into artificially prepared phospholipid bilayers. Furthermore, the isolated mitochondria can be used for assaying the mitochondrial permeability transition pore (MPTP).
authors.affiliation: Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
authors.forename: Danya
authors.initials: D
authors.lastname: Ben-Hail
authors.forename: Varda
authors.initials: V
authors.lastname: Shoshan-Barmatz
mesh_terms: D000818:Animals; D008051:Lipid Bilayers / Q000378:metabolism; D008930:Mitochondria, Liver / Q000201:enzymology*; D051336:Mitochondrial Membranes / Q000502:physiology; D010539:Permeability; D051381:Rats; D050995:Voltage-Dependent Anion Channel 1 / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D008051:Lipid Bilayers; C494764:Vdac1 protein, rat; D050995:Voltage-Dependent Anion Channel 1