title: Photodamage in a mitochondrial membrane model modulated by the topology of cationic and anionic meso-tetrakis porphyrin free bases.
abstract: The photodynamic effects of the cationic TMPyP (meso-tetrakis [N-methyl-4-pyridyl]porphyrin) and the anionic TPPS4 (meso-tetrakis[4-sulfonatophenyl]porphyrin) against PC/CL phosphatidylcholine/cardiolipin (85/15%) membranes were probed to address the influence of phorphyrin binding on lipid damage. Electronic absorption spectroscopy and zeta potential measurements demonstrated that only TMPyP binds to PC/CL large unilamellar vesicles (LUVs). The photodamage after irradiation with visible light was analyzed by dosages of lipid peroxides (LOOH) and thiobarbituric reactive substance and by a contrast phase image of the giant unilamellar vesicles (GUVs). Damage to LUVs and GUVs promoted by TMPyP and TPPS4 were qualitatively and quantitatively different. The cationic porphyrin promoted damage more extensive and faster. The increase in LOOH was higher in the presence of D2O, and was impaired by sodium azide and sorbic acid. The effect of D2O was higher for TPPS4 as the photosensitizer. The use of DCFH demonstrated that liposomes prevent the photobleaching of TMPyP. The results are consistent with a more stable TMPyP that generates long-lived singlet oxygen preferentially partitioned in the bilayer. Conversely, TPPS4 generates singlet oxygen in the bulk whose lifetime is increased in D2O. Therefore, the affinity of the porphyrin to the membrane modulates the rate, type and degree of lipid damage.
authors.affiliation: Centro de Ciências Naturais e Humanas (CCNH), Universidade Federal do ABC, Santo André, SP, Brazil.
authors.forename: Cintia
authors.initials: C
authors.lastname: Kawai
authors.forename: Juliana C
authors.initials: JC
authors.lastname: Araújo-Chaves
authors.forename: Taciana
authors.initials: T
authors.lastname: Magrini
authors.forename: Camila O C C
authors.initials: CO
authors.lastname: Sanches
authors.forename: Sandra M S
authors.initials: SM
authors.lastname: Pinto
authors.forename: Herculano
authors.initials: H
authors.lastname: Martinho
authors.forename: Nasser
authors.initials: N
authors.lastname: Daghastanli
authors.forename: Iseli L
authors.initials: IL
authors.lastname: Nantes
mesh_terms: D001412:Bacillus subtilis / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007700:Kinetics; D013171:Spores, Bacterial / Q000187:drug effects* / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D006861:Hydrogen Peroxide