title: Purification and characterization of a novel C-type hemolytic lectin for clot lysis from the fresh water clam Villorita cyprinoides: a possible natural thrombolytic agent against myocardial infarction.
abstract: Villorita cyprinoides (black clam) is a fresh water clam that belongs as a bivalve to the group of mollusc. The saline extracts from the muscle reveal high titers of agglutination potency on trypsin-treated rabbit erythrocytes. With the help of affinity chromatography a hemolytic protein with lectin activity which could all be inhibited by D-galactose were isolated. The lectins were separated on DEAE-cellulose and the main component was purified after an additional step of gel filtration on sephadex G-75. The main component is a non-glycosylated protein with a molecular weight of 96,560 Da determined by MALDI-ToF, consisting of a single protein chain and characterized by the lack of polymers and intermediate disulfide bonds. The pure main lectin with clot lytic feature shows two bands at molecular weights 36,360 and 26, 520 Da. Optimal inhibition of the pure lectin is achieved by D-galactose containing oligo- and polysaccharides. The lectin activity decreased above 40 °C and was lost at 62 °C, the stability over the pH range between 7.0 and 8.0 and requires divalent cations for their activity. The novel C-type hemolytic lectin for clot lysis from the clam Villorita cyprinoides was identified and evaluated, the purified hemolytic lectin (0.35 mg/ml and 0.175 mg/ml) enhanced clot lysis activity when compared to the different concentration (5 mg/ml and 1 mg/ml) of commercial streptokinase. In the present study identified hemolytic lectin was a rapid and effective clot lytic molecule and could be developed as new drug molecule in future.
authors.affiliation: International Centre for Nanobiotechnology (ICN), CMST Campus, Manonmaniam Sundaranar University, Rajakkamanagalam, Kanyakumari Dist., 629502 TN, India; Xpression Biotek (Pvt) Ltd., Marthandam, Kanyakumari Dist., TN, India. Electronic address: learnals@yahoo.co.in.
authors.forename: G R Learnal
authors.initials: GR
authors.lastname: Sudhakar
authors.affiliation: International Centre for Nanobiotechnology (ICN), CMST Campus, Manonmaniam Sundaranar University, Rajakkamanagalam, Kanyakumari Dist., 629502 TN, India. Electronic address: vsgprakash.icn@gmail.com.
authors.forename: S G Prakash
authors.initials: SG
authors.lastname: Vincent
mesh_terms: D000371:Agglutination / Q000187:drug effects; D000818:Animals; D049872:Bivalvia / Q000737:chemistry* / Q000235:genetics; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D055808:Drug Discovery; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects; D005343:Fibrinolytic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D037102:Lectins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D009203:Myocardial Infarction / Q000517:prevention & control; D011817:Rabbits; D013300:Streptokinase / Q000494:pharmacology
chemical_list: D005343:Fibrinolytic Agents; D037102:Lectins; D013300:Streptokinase
keywords: Clam; Clot lysis; Hemolytic lectin; MALDI-ToF; Streptokinase