title: Method for detecting the reactivity of chemicals towards peptides as an alternative test method for assessing skin sensitization potential.
abstract: Cosmetics are normally composed of various ingredients. Some cosmetic ingredients can act as chemical haptens reacting toward proteins or peptides of human skin and they can provoke an immunologic reaction, called as skin sensitization. This haptenation process is very important step of inducing skin sensitization and evaluating the sensitizing potentials of cosmetic ingredients is very important for consumer safety. Therefore, animal alternative methods focusing on monitoring haptenation potential are undergoing vigorous research. To examine the further usefulness of spectrophotometric methods to monitor reactivity of chemicals toward peptides for cosmetic ingredients. Forty chemicals (25 sensitizers and 15 non-sensitizers) were reacted with 2 synthetic peptides, e.g., the cysteine peptides (Ac-RFAACAA-COOH) with free thiol group and the lysine peptides (Ac-RFAAKAA-COOH) with free amine group. Unreacted peptides can be detected after incubating with 5,5'-dithiobis-2-nitrobenzoic acid or fluorescamine™ as detection reagents for free thiol and amine group, respectively. Chemicals were categorized as sensitizers when they induced more than 10% depletion of cysteine peptides or more than 30% depletion of lysine peptides. The sensitivity, specificity, and accuracy were 80.0%, 86.7% and 82.5%, respectively. These results demonstrate that spectrophotometric methods can be an easy, fast, and high-throughput screening tools predicting the skin sensitization potential of chemical including cosmetic ingredient.
authors.affiliation: AmorePacific Corporation R&D Unit, Yongin-si, Republic of Korea.
authors.forename: Sun-A
authors.initials: SA
authors.lastname: Cho
authors.affiliation: AmorePacific Corporation R&D Unit, Yongin-si, Republic of Korea.
authors.forename: Yun Hyeok
authors.initials: YH
authors.lastname: Jeong
authors.affiliation: AmorePacific Corporation R&D Unit, Yongin-si, Republic of Korea.
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Kim
authors.affiliation: AmorePacific Corporation R&D Unit, Yongin-si, Republic of Korea.
authors.forename: Seoyoung
authors.initials: S
authors.lastname: Kim
authors.affiliation: AmorePacific Corporation R&D Unit, Yongin-si, Republic of Korea; Division of Chemical Engineering, College of Engineering, Hanyang University, Seoul, Republic of Korea.
authors.forename: Jun-Cheol
authors.initials: JC
authors.lastname: Cho
authors.forename: Yong
authors.initials: Y
authors.lastname: Heo
authors.affiliation: College of Natural Sciences, Catholic University of Daegu, Kyongsan-si, Republic of Korea.
authors.forename: Young
authors.initials: Y
authors.lastname: Heo
authors.affiliation: Division of Chemical Engineering, College of Engineering, Hanyang University, Seoul, Republic of Korea.
authors.forename: Kyung-Do
authors.initials: KD
authors.lastname: Suh
authors.affiliation: AmorePacific Corporation R&D Unit, Yongin-si, Republic of Korea. Electronic address: rsj001@amorepacific.com.
authors.forename: Kyeho
authors.initials: K
authors.lastname: Shin
authors.forename: Susun
authors.initials: S
authors.lastname: An
mesh_terms: D003358:Cosmetics / Q000737:chemistry / Q000145:classification / Q000633:toxicity*; D003545:Cysteine; D006241:Haptens / Q000737:chemistry / Q000145:classification / Q000633:toxicity*; D057166:High-Throughput Screening Assays; D008239:Lysine; D009842:Oligopeptides / Q000737:chemistry*; D023422:Skin Irritancy Tests / Q000379:methods*; D013053:Spectrophotometry
chemical_list: D003358:Cosmetics; D006241:Haptens; D009842:Oligopeptides; D008239:Lysine; D003545:Cysteine
keywords: Cosmetic ingredient; Peptide assay; Skin sensitization; Spectrophotometric method