title: Detection of hepatitis B virus infection markers in dried plasma spots among patients in Congo-Brazzaville.
abstract: The detection of hepatitis B virus (HBV) infection markers by using dried plasma spots from 32 patients living in Congo has been assessed. Considering frozen plasma samples as gold standard, the sensitivity and specificity of HBV serologic markers detection in dried plasma eluted from filter paper were 100%. The sensitivity and the specificity of HBV DNA detection reached 96% and 100%, respectively, with plasma samples dried on filter paper compared to standard samples. Dried plasma samples can represent an alternative to conventional sampling for HBV detection and management of the infection in developing countries.
authors.affiliation: Université Lille 2, Faculté de Médecine, CHRU Lille, Laboratoire de virologie EA3610, Lille, France.
authors.forename: Enagnon Kazali
authors.initials: EK
authors.lastname: Alidjinou
authors.affiliation: Hôpital Général de Loandjili, Pointe Noire, Congo.
authors.forename: Donatien
authors.initials: D
authors.lastname: Moukassa
authors.affiliation: Université Lille 2, Faculté de Médecine, CHRU Lille, Laboratoire de virologie EA3610, Lille, France.
authors.forename: Famara
authors.initials: F
authors.lastname: Sané
authors.affiliation: Hôpital Général de Loandjili, Pointe Noire, Congo.
authors.forename: Séraphin
authors.initials: S
authors.lastname: Twagirimana Nyenyeli
authors.affiliation: Hôpital Général de Loandjili, Pointe Noire, Congo.
authors.forename: Estina Chandrelle
authors.initials: EC
authors.lastname: Akoko
authors.affiliation: Hôpital Général de Loandjili, Pointe Noire, Congo.
authors.forename: Michèle Valy
authors.initials: MV
authors.lastname: Mountou
authors.affiliation: Université Lille 2, Faculté de Médecine, CHRU Lille, Laboratoire de virologie EA3610, Lille, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Bocket
authors.affiliation: Centre Hospitalier et Universitaire de Brazzaville (CHUB), Brazzaville, Congo.
authors.forename: Jean-Rosaire
authors.initials: JR
authors.lastname: Ibara
authors.affiliation: Université Lille 2, Faculté de Médecine, CHRU Lille, Laboratoire de virologie EA3610, Lille, France. Electronic address: didier.hober@chru-lille.fr.
authors.forename: Didier
authors.initials: D
authors.lastname: Hober
mesh_terms: D003223:Congo; D004279:DNA, Viral / Q000097:blood*; D006509:Hepatitis B / Q000175:diagnosis*; D006515:Hepatitis B virus / Q000302:isolation & purification; D006801:Humans; D010949:Plasma / Q000821:virology*; D012680:Sensitivity and Specificity; D013048:Specimen Handling
chemical_list: D004279:DNA, Viral
keywords: Congo-Brazzaville; Dried plasma spots; HBV DNA; HBV antibodies; HBV antigens