title: Sequential accumulation of iron in glial cells during chicken cerebellar development.
abstract: Iron is an essential, but potentially harmful, metal in the brain. In normal brain, iron has been reported to accumulate mainly in glial cells and occasionally in neurons in some particular nuclei. However, the majority of investigations have targeted the adult brain. Here, we investigated spatiotemporal localization of iron in developing and adult chicken cerebellum using iron histochemistry. Iron reactivity was not detected in the chick cerebellum until embryonic day 12. Iron accumulation was first found in mature myelinating oligodendrocytes located in the inner part of the cerebellar folium at embryonic day 14. From embryonic day 20, iron-positive mature myelinating oligodendrocytes were localized in the white matter and the granular layer. From post-hatching day 2, iron accumulation was observed in Bergmann glia in the Purkinje cell layer as well as in mature myelinating oligodendrocytes. Iron accumulation in microglia was observed in the granular and molecular layers at post-hatching month 12. Our data indicate that during cerebellar development iron is accumulated in a unique sequence according to individual requirements or microenvironmental demands.
authors.affiliation: Department of Anatomy, Chungbuk National University School of Medicine, Cheongju 361-763, South Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Department of Anatomy, Chungbuk National University School of Medicine, Cheongju 361-763, South Korea.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Lee
authors.affiliation: Department of Anatomy, Chungbuk National University School of Medicine, Cheongju 361-763, South Korea.
authors.forename: Hyun Joon
authors.initials: HJ
authors.lastname: Sohn
authors.affiliation: Department of Anatomy, Chungnam National University School of Medicine, Daejeon 301-747, South Korea.
authors.forename: Dong Woon
authors.initials: DW
authors.lastname: Kim
authors.affiliation: Department of Anatomy, Jeju National University School of Medicine, Jeju 690-756, South Korea. Electronic address: chossn@snu.ac.kr.
authors.forename: Sa Sun
authors.initials: SS
authors.lastname: Cho
authors.affiliation: Department of Anatomy, Chungbuk National University School of Medicine, Cheongju 361-763, South Korea. Electronic address: seojh@chungbuk.ac.kr.
authors.forename: Je Hoon
authors.initials: JH
authors.lastname: Seo
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology; D002531:Cerebellum / Q000196:embryology* / Q000473:pathology; D002642:Chick Embryo; D002645:Chickens; D007501:Iron / Q000378:metabolism*; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009836:Oligodendroglia / Q000378:metabolism
chemical_list: D007501:Iron
keywords: Bergmann glia; Cerebellum; Chicken; Iron; Microglia; Oligodendrocyte