title: Enzymatic methods for the determination of pollution in seawater using salt resistant alkaline phosphatase from eggs of the sea urchin Strongylocentrotus intermedius.
abstract: A new salt resistant alkaline phosphatase from eggs of the sea urchin Strongylocentrotus intermedius (StAP) has been shown to have a unique property to hydrolyze substrate in seawater without loss of enzymatic activity. The enzyme has pH optimum at 8.0-8.5. Model experiments showed various concentrations of copper, zinc, cadmium and lead added to seawater or a standard buffer mixture to inhibit completely the enzyme activity at the concentrations of 15-150 μg/l. StAP sensitivity to the presence in seawater of metals, pesticides, detergents and oil products appears to be considerably less. Samples of seawater taken from aquatic areas of the Troitsy Bay of the Peter the Great Bay, Japan Sea have been shown to inhibit the enzyme activity; the same was shown for the samples of fresh waters. The phosphatase inhibition assay developed proved to be highly sensitive, technically easy-to use allowing to test a great number of samples.
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Science, Vladivostok, Russian Federation, 159, Stoletya Vladivostoku Str., Vladivostok 690022, Russia.
authors.forename: Natalie I
authors.initials: NI
authors.lastname: Menzorova
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Science, Vladivostok, Russian Federation, 159, Stoletya Vladivostoku Str., Vladivostok 690022, Russia. Electronic address: sasha0788@inbox.ru.
authors.forename: Alexandra V
authors.initials: AV
authors.lastname: Seitkalieva
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Science, Vladivostok, Russian Federation, 159, Stoletya Vladivostoku Str., Vladivostok 690022, Russia.
authors.forename: Valerу A
authors.initials: VA
authors.lastname: Rasskazov
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry*; D000818:Animals; D004784:Environmental Monitoring / Q000379:methods*; D057075:Enzyme Assays / Q000379:methods*; D007564:Japan; D008670:Metals / Q000032:analysis; D010063:Ovum / Q000378:metabolism; D010575:Pesticides / Q000032:analysis; D012623:Seawater / Q000737:chemistry*; D012965:Sodium Chloride; D047269:Strongylocentrotus / Q000502:physiology; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D008670:Metals; D010575:Pesticides; D014874:Water Pollutants, Chemical; D012965:Sodium Chloride; D000469:Alkaline Phosphatase
keywords: Alkaline phosphatase (AP); Eggs of sea urchin; Japan Sea; Phosphatase inhibition assay; Pollution monitoring; Strongylocentrotus intermedius