title: Dehydroalanine-based diubiquitin activity probes.
abstract: A strategy for the synthesis of dehydroalanine based diubiquitin activity probes is described. The site-specific introduction of dehydroalanine was achieved from diubiquitin bearing Cys residue near the scissile bond between two ubiquitins linked through Lys48, Lys63 or in a head to tail fashion. The probes were characterized for their activities with various deubiquitinases, which open new opportunities in studying deubiquitinases in various settings.
authors.affiliation: Department of Chemistry, Ben-Gurion University of the Negev , Beer Sheva 84105, Israel.
authors.forename: Najat
authors.initials: N
authors.lastname: Haj-Yahya
authors.forename: Hosahalli P
authors.initials: HP
authors.lastname: Hemantha
authors.forename: Roman
authors.initials: R
authors.lastname: Meledin
authors.forename: Somasekhar
authors.initials: S
authors.lastname: Bondalapati
authors.forename: Mallikanti
authors.initials: M
authors.lastname: Seenaiah
authors.forename: Ashraf
authors.initials: A
authors.lastname: Brik
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives* / Q000737:chemistry; D000595:Amino Acid Sequence; D006801:Humans; D008239:Lysine / Q000737:chemistry; D008958:Models, Molecular; D015394:Molecular Structure; D011487:Protein Conformation; D064570:Ubiquitin-Specific Proteases / Q000378:metabolism*; D014452:Ubiquitins / Q000378:metabolism*
chemical_list: C109184:UBD protein, human; D014452:Ubiquitins; C015102:dehydroalanine; D064570:Ubiquitin-Specific Proteases; D008239:Lysine; D000409:Alanine