title: Simultaneous fluorescence sensing of Cys and GSH from different emission channels.
abstract: A chlorinated coumarin-hemicyanine dye with three potential reaction sites was exploited as fluorescent probe for biothiols. The Cys-induced substitution-rearrangement-cyclization, Hcy-induced substitution-rearrangement, and GSH-induced substitution-cyclizatioin cascades lead to the corresponding amino-coumarin, amino-coumarin-hemicyanine, thiol-coumarin with distinct photophysical properties, enabling Cys and GSH to be selectively detected from different emission channels at two different excitation wavelengths.
authors.affiliation: School of Chemistry and Chemical Engineering and ‡Institute of Biotechnology, Shanxi University , Taiyuan 030006, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Yuan-Qiang
authors.initials: YQ
authors.lastname: Sun
authors.forename: Yingying
authors.initials: Y
authors.lastname: Huo
authors.forename: Hongxing
authors.initials: H
authors.lastname: Zhang
authors.forename: Linfang
authors.initials: L
authors.lastname: Wang
authors.forename: Pei
authors.initials: P
authors.lastname: Zhang
authors.forename: Dan
authors.initials: D
authors.lastname: Song
authors.forename: Yawei
authors.initials: Y
authors.lastname: Shi
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
mesh_terms: D000818:Animals; D019556:COS Cells; D002232:Carbocyanines / Q000737:chemistry*; D002522:Chlorocebus aethiops; D003374:Coumarins / Q000737:chemistry*; D003486:Cyanides / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005978:Glutathione / Q000032:analysis*; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D002232:Carbocyanines; D003374:Coumarins; D003486:Cyanides; D005456:Fluorescent Dyes; D005978:Glutathione