title: [Source and effect of IL-22 in graft versus host disease mouse after allo-genetic bone marrow transplantation].
abstract: This study was purposed to explore the cellular source of IL-22 in graft versus host disease (GVHD) mouse following allo-genetic bone marrow transplantation. BALB/c and C57BL/6 mice were used as recipients and donors, respectively. GVHD model was established by irradiated BABL/c mice inoculated with mixed suspension of C57BL/6 bone marrow cells and splenic lymphocytes. The mice were divided into normal group (normal), total body irradiated group (TBI), bone marrow cell-transplanted group (BMT), and the combination of bone marrow cell and splenic lymphocytes-induced GVHD group (BS). The level of IL-22 in plasma was detected by ELISA. The cellular source of IL-22 and IL-22(+) subsets were detected by flow cytometry. The results showed that compared with normal mice, the level of IL-22 in plasma from BS mice was the highest (P < 0.01). All the lymphocytes of spleen, lymph nodes and peripheral blood from BS mice could produce IL-22, in which the percentage of IL-22(+)CD4(+) T cell was higher than that of IL-22(+)CD8(+) T cells. Not only Th22 cells but also Th1 and Th17 cells were the cellular source of IL-22 in GVHD mice. It is concluded that the high level of IL-22 has been found in mice with GVHD, which mainly originates from IFN-γ(-)IL-17(-)IL-22(+) Th22 cells.
authors.affiliation: Laboratory of Transplantation Immunology, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China; Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Huang
authors.affiliation: Laboratory of Transplantation Immunology, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Zhao
authors.affiliation: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Chen
authors.affiliation: Laboratory of Transplantation Immunology, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Qing-Yun
authors.initials: QY
authors.lastname: Wu
authors.affiliation: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Pan
authors.affiliation: Laboratory of Transplantation Immunology, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Ling-Yu
authors.initials: LY
authors.lastname: Zeng
authors.affiliation: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Li
authors.affiliation: Laboratory of Transplantation Immunology, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China; Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China. E-mail:lihmd@163.com.
authors.forename: Kai-Lin
authors.initials: KL
authors.lastname: Xu
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000009:adverse effects* / Q000379:methods; D005260:Female; D006086:Graft vs Host Disease / Q000097:blood*; D007378:Interleukins / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D014184:Transplantation, Homologous; D000097043:Interleukin-22
chemical_list: D007378:Interleukins; D000097043:Interleukin-22