title: Physicochemical factors affecting the stability of two pigments: R-phycoerythrin of Grateloupia turuturu and B-phycoerythrin of Porphyridium cruentum.
abstract: Phycoerythrin is a major light-harvesting pigment of red algae, which could be used as a natural dye in foods. The stability of R-phycoerythrin of Grateloupia turuturu and B-phycoerythrin of Porphyridium cruentum in relation to different light exposure times, pHs, and temperatures was studied. Regarding the light exposure time, after 48h, the reduction in concentrations of B-phycoerythrin and R-phycoerythrin were 30±2.4% and 70±1%, respectively. Phycoerythrins presented good stability from pH 4 to 10. At pH 2, the reduction in concentration was 90±4% for B-phycoerythrin and 40±2.5% for R-phycoerythrin while, at pH 12, the phycoerythrins were degraded. Phycoerythrins showed good stability toward temperature, up to 40°C. At 60°C, the reduction in concentrations of B-phycoerythrin and R-phycoerythrin were 50±3.4% and 70±0.18%, respectively. Moreover, the best conditions of storage (-20°C) were determined.
authors.affiliation: Mer Molécule Santé, EA 2160, Université de Nantes, Pôle Mer et Littoral, 2 rue de la Houssinière, BP 92208, 44322 Nantes Cedex 3, France.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Munier
authors.affiliation: Laboratoire de Génie des Procédés Environnement Agro-alimentaire (GEPEA), UMR CNRS 6144, Université de Nantes, Pôle Mer et Littoral, 37 Boulevard de l'Université, BP 406, 44602 Saint-Nazaire Cedex, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Jubeau
authors.affiliation: Mer Molécule Santé, EA 2160, Université de Nantes, Pôle Mer et Littoral, 2 rue de la Houssinière, BP 92208, 44322 Nantes Cedex 3, France.
authors.forename: Alva
authors.initials: A
authors.lastname: Wijaya
authors.affiliation: Mer Molécule Santé, EA 2160, Université de Nantes, Pôle Mer et Littoral, 2 rue de la Houssinière, BP 92208, 44322 Nantes Cedex 3, France.
authors.forename: Michèle
authors.initials: M
authors.lastname: Morançais
authors.affiliation: Mer Molécule Santé, EA 2160, Université de Nantes, Pôle Mer et Littoral, 2 rue de la Houssinière, BP 92208, 44322 Nantes Cedex 3, France.
authors.forename: Justine
authors.initials: J
authors.lastname: Dumay
authors.affiliation: Laboratoire de Génie des Procédés Environnement Agro-alimentaire (GEPEA), UMR CNRS 6144, Université de Nantes, Pôle Mer et Littoral, 37 Boulevard de l'Université, BP 406, 44602 Saint-Nazaire Cedex, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Marchal
authors.affiliation: Laboratoire de Génie des Procédés Environnement Agro-alimentaire (GEPEA), UMR CNRS 6144, Université de Nantes, Pôle Mer et Littoral, 37 Boulevard de l'Université, BP 406, 44602 Saint-Nazaire Cedex, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Jaouen
authors.affiliation: Mer Molécule Santé, EA 2160, Université de Nantes, Pôle Mer et Littoral, 2 rue de la Houssinière, BP 92208, 44322 Nantes Cedex 3, France. Electronic address: joel.fleurence@univ-nantes.fr.
authors.forename: Joël
authors.initials: J
authors.lastname: Fleurence
mesh_terms: D006863:Hydrogen-Ion Concentration; D008027:Light; D010799:Phycoerythrin / Q000737:chemistry*; D010860:Pigments, Biological / Q000737:chemistry*; D044665:Porphyridium / Q000737:chemistry*; D000461:Rhodophyta / Q000737:chemistry*; D013696:Temperature
chemical_list: D010860:Pigments, Biological; D010799:Phycoerythrin
keywords: B-PE; B-phycoerythrin; CE; Grateloupia turuturu; Light stability; PBP; PE; PI; Phycoerythrins; Porphyridium cruentum; R-PE; R-phycoerythrin; Temperature stability; crude extract; pH stability; phycobiliprotein; phycoerythrin; purity index