title: Influence of extract of Ginkgo biloba leaves tablets on the aquaporin-1 expression in isolated lung ischemia reperfusion.
abstract: BACKGROUND
  The extract of Ginkgo biloba leaves tablets, ginaton, is widely used in treating ischemic cerebrovascular disease in the clinic. This study aimed to investigate the expression of aquaporin-1 (AQP-1) in rat lung with ischemia/reperfusion injury after pretreatment with ginaton, and whether the pretreatment with ginaton reduces the acute lung injury caused by ischemia/reperfusion injury.
  
  
  METHODS
  Adult Wistar rats were divided into two groups. Some rats were used as donors (n = 20), the others as recipients (n = 20). Left lungs of donor rats were used for the isolated lung reperfusion model, which perfused only with low potassium dextran (LPD) solution as group A (n = 10); the others were pretreated with ginaton before reperfusion as group C (n = 10). Right lung of donor rat without any treatment was used as a control group (group B and group D, n = 10 for each group). After the model was established, the expression of AQP-1 in the lung tissues was examined by immunohistochemistry, Western blotting, and reverse transcriptase-polymerase chain reaction.
  
  
  RESULTS
  Immunohistochemical examination revealed that AQP-1 was expressed in endothelia. Immunoblotting demonstrated that the relative gray values of AQP-1 protein in groups A and C were 0.65±0.06, 0.88±0.11, respectively. The relative gray values of the mRNA expression in groups A and C were 0.30±0.08, 0.49±0.11, respectively. The expression of AQP-1 protein and mRNA in group C was significantly higher than in group A (P < 0. 05).
  
  
  CONCLUSIONS
  The pretreatment with ginaton can reduce the acute lung injury caused by ischemia/reperfusion.
authors.affiliation: Department of Thoracic Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Xiang-Nan
authors.initials: XN
authors.lastname: Li
authors.affiliation: Department of Histology and Embryology, Basic Medical College, Zhengzhou University, Zhengzhou, Henan 450001, China.
authors.forename: Ji-Yao
authors.initials: JY
authors.lastname: Yang
authors.affiliation: Nursing College of Zhengzhou University, Zhengzhou, Henan 450052, China. Email: panxue2000@126.com.
authors.forename: Xue
authors.initials: X
authors.lastname: Pan
authors.affiliation: Department of Thoracic Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Song
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Thoracic Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Chun-Yang
authors.initials: CY
authors.lastname: Zhang
authors.affiliation: Department of Thoracic Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Deng-Yan
authors.initials: DY
authors.lastname: Zhu
authors.affiliation: Nursing College of Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
mesh_terms: D000818:Animals; D051398:Aquaporin 1 / Q000235:genetics / Q000378:metabolism*; D020441:Ginkgo biloba / Q000737:chemistry*; D007150:Immunohistochemistry; D008168:Lung / Q000187:drug effects* / Q000378:metabolism*; D010936:Plant Extracts / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy / Q000378:metabolism*; D013607:Tablets
chemical_list: D010936:Plant Extracts; D013607:Tablets; D051398:Aquaporin 1