title: Is the oxidative DNA damage level of human lymphocyte correlated with the antioxidant capacity of serum or the base excision repair activity of lymphocyte?
abstract: A random screening of human blood samples from 24 individuals of nonsmoker was conducted to examine the correlation between the oxidative DNA damage level of lymphocytes and the antioxidant capacity of serum or the base excision repair (BER) activity of lymphocytes. The oxidative DNA damage level was measured with comet assay containing Fpg/Endo III cleavage, and the BER activity was estimated with a modified comet assay including nuclear extract of lymphocytes for enzymatic cleavage. Antioxidant capacity was determined with trolox equivalent antioxidant capacity assay. We found that though the endogenous DNA oxidation levels varied among the individuals, each individual level appeared to be steady for at least 1 month. Our results indicate that the oxidative DNA damage level is insignificantly or weakly correlated with antioxidant capacity or BER activity, respectively. However, lymphocytes from carriers of Helicobacter pylori (HP) or Hepatitis B virus (HBV) tend to give higher levels of oxidative DNA damage (P < 0.05). Though sera of this group of individuals show no particular tendency with reduced antioxidant capacity, the respective BER activities of lymphocytes are lower in average (P < 0.05). Thus, reduction of repair activity may be associated with the genotoxic effect of HP or HBV infection.
authors.affiliation: Institute of Molecular Medicine, National Tsing-Hua University, Hsinchu 30013, Taiwan.
authors.forename: Yi-Chih
authors.initials: YC
authors.lastname: Tsai
authors.affiliation: Department of Pathology and Laboratory Medicine, Hsinchu Mackay Memorial Hospital, Hsinchu 30013, Taiwan.
authors.forename: Pei-Yi
authors.initials: PY
authors.lastname: Li
authors.affiliation: Department of GI and Hepatology, Hsinchu Mackay Memorial Hospital, Hsinchu 30013, Taiwan.
authors.forename: Chung-Chu
authors.initials: CC
authors.lastname: Chen
authors.affiliation: Institute of Molecular Medicine, National Tsing-Hua University, Hsinchu 30013, Taiwan.
authors.forename: Yin-Chang
authors.initials: YC
authors.lastname: Liu
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D002457:Cell Extracts; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair; D005260:Female; D016481:Helicobacter Infections / Q000473:pathology; D006509:Hepatitis B / Q000473:pathology; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D010084:Oxidation-Reduction; D044967:Serum / Q000378:metabolism*; D055815:Young Adult
chemical_list: D000975:Antioxidants; D002457:Cell Extracts