title: Establishing a bone marrow stromal cell transplant program at the National Institutes of Health Clinical Center.
abstract: A repository of cryopreserved bone marrow stromal cell (BMSC) products prepared from marrow aspirates of healthy subjects has been created and is being used to treat patients with inflammatory bowel disease, cardiovascular disease, and acute graft-versus-host disease following allogeneic hematopoietic stem cell transplantation. New methods of manufacturing BMSCs are being investigated including the use of an automated bioreactor for BMSC expansion and the replacement of fetal bovine serum with human platelet lysate as a media supplement. Efforts are also being made to identify markers that can be used to assess the potency of BMSCs.
authors.affiliation: 1 Department of Transfusion Medicine, Clinical Center, National Institutes of Health , Bethesda, Maryland.
authors.forename: David F
authors.initials: DF
authors.lastname: Stroncek
authors.forename: Marianna
authors.initials: M
authors.lastname: Sabatino
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Ren
authors.forename: Lee
authors.initials: L
authors.lastname: England
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Kuznetsov
authors.forename: Harvey G
authors.initials: HG
authors.lastname: Klein
authors.forename: Pamela G
authors.initials: PG
authors.lastname: Robey
mesh_terms: D000818:Animals; D061249:Batch Cell Culture Techniques; D018070:Biological Specimen Banks / Q000458:organization & administration*; D002478:Cells, Cultured; D006078:Government Programs / Q000458:organization & administration*; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D009316:National Institutes of Health (U.S.) / Q000458:organization & administration*; D057905:Stem Cell Research; D023822:Tissue Engineering; D014481:United States