title: A novel LC-MS/MS method for determination of tissue distribution and excretion of timosaponin B-II in rat biological matrices.
abstract: Timosaponin B-II (TB-II) is a natural bioactive steroid glycoside extracted from the Chinese medicinal herb Anemarrhena asphodeloides Bge. (Fam. Liliaceae). It has been demonstrated to have a good anti-inflammatory effect and a low bioavailability (1.1%). Clinical research has focused on developing it into a completely new medicine. In this study, a rapid and sensitive analytical method based on LC-MS/MS has been developed for the determination of TB-II in rat biological matrices (tissues, bile, urine and feces samples). The analytes and internal standard were isolated from 100 μL samples by solid-phase extraction and then separated using a DIKMA Inertsil ODS-3 column (5 µm, 2.1 × 150 mm) with an isocratic mobile phase consisting of acetonitrile-0.05% formic acid (35:65) at a flow rate of 0.25 mL/min. Calibration curves (1/χ(2) -weighted) offered satisfactory linearity (r(2)  ≥ 0.990) within the test range. The accuracy, precision, recoveries and matrix effects were satisfactory in all the biological matrices examined. The assay was successfully applied to a tissue distribution and excretion study in rats. The preclinical data are useful for the design of clinical trials of TB-II.
authors.affiliation: Department of Pharmacy, Changzheng Hospital, Second Military Medical University, No. 415 Fengyang Road, Shanghai, 200003, People's Republic of China; Center of Drug Clinical Trials, Nanfang Hospital, Southern Medical University, No. 1838 Northern Guangzhou Avenue, Guangzhou, 510515, People's Republic of China.
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Xu
authors.forename: Shouhong
authors.initials: S
authors.lastname: Gao
authors.forename: Fei
authors.initials: F
authors.lastname: Cai
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Liu
authors.forename: Wansheng
authors.initials: W
authors.lastname: Chen
mesh_terms: D000818:Animals; D001646:Bile / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D005243:Feces / Q000737:chemistry; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics*; D012680:Sensitivity and Specificity; D013256:Steroids / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D012503:Saponins; D013256:Steroids; C523027:timosaponin B-II
keywords: LC-MS/MS; excretion; rats; timosaponin B-II; tissue distribution