title: Cardenolide glycosides from Elaeodendron australe var. integrifolium.
abstract: Extracts from dried leaf and stems of Elaeodendron australe var. integrifolium (Celastraceae) collected in South East Queensland, Australia, were active in an assay that measured Ca(2+) driven expression of IL-2/luciferase designed to identify inhibitors of the ICRAC channel. Bioassay-guided isolation using C18 and polyamide column chromatography, HPLC (Phenyl and C18) and centrifugal partition chromatography (CPC) led to the isolation of digitoxigenin (1) and three cardenolide glycosides, glucoside 2, quinovoside 3 and the new natural product xyloside 4, as the active components with low nM activity in the reporter assay.
authors.affiliation: Eskitis Institute, Griffith University, Brisbane, Queensland 4111, Australia.
authors.forename: Mark S
authors.initials: MS
authors.lastname: Butler
authors.affiliation: Eskitis Institute, Griffith University, Brisbane, Queensland 4111, Australia.
authors.forename: Leanne
authors.initials: L
authors.lastname: Towerzey
authors.affiliation: Eskitis Institute, Griffith University, Brisbane, Queensland 4111, Australia.
authors.forename: Ngoc B
authors.initials: NB
authors.lastname: Pham
authors.affiliation: Eskitis Institute, Griffith University, Brisbane, Queensland 4111, Australia.
authors.forename: Edward
authors.initials: E
authors.lastname: Hyde
authors.affiliation: Eskitis Institute, Griffith University, Brisbane, Queensland 4111, Australia.
authors.forename: Sao Khemar
authors.initials: SK
authors.lastname: Wadi
authors.affiliation: Queensland Herbarium, Department of Science, Information Technology, Innovation and the Arts, Brisbane Botanic Gardens, Brisbane, Australia.
authors.forename: Gordon P
authors.initials: GP
authors.lastname: Guymer
authors.affiliation: Eskitis Institute, Griffith University, Brisbane, Queensland 4111, Australia. Electronic address: r.quinn@griffith.edu.au.
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Quinn
mesh_terms: D015220:Calcium Channels / Q000378:metabolism*; D002298:Cardenolides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D029753:Celastraceae / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008968:Molecular Conformation; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D015220:Calcium Channels; D002298:Cardenolides; D006027:Glycosides
keywords: Cardenolide; Celastraceae; Centrifugal partition chromatography; Elaeodendron australe var. integrifolium; Interleukin-2 (IL-2)/luciferase inhibitor; Ion channel; Na(+)/K(+)-ATPase