title: Helicobacter pylori genotypes associated with gastric histo-pathological damages in a Moroccan population.
abstract: H. pylori persistent infection induces chronic gastritis and is associated with peptic ulcer disease and gastric carcinoma development. The severity of these diseases is related to human's genetic diversity, H. pylori genetic variability and environmental factors. To identify the prevalence of histo-pathological damages caused by H. pylori infection in Moroccan population, and to determine their association to H. pylori genotypes, a prospective study has been conducted during 3 years on patients attending the gastroenterology department of Hassan II University Hospital (CHU) of Fez, Morocco. A total of 801 Moroccan adults' patients were recruited; H. pylori was diagnosed and genotyped by PCR in biopsy specimens and histological exam was performed. We found a high rate of glandular atrophy. Chronic inflammation, neutrophil activity and glandular atrophy showed statistically significant association with H. pylori infection. However, intestinal metaplasia was inversely associated to this infection and no association was observed with gastric cancer cases. A statistically significant association was found between intestinal metaplasia and vacAs1 and vac Am1 genotypes in patients aged 50 years and more but not in younger. This last genotype is also associated to gastric cancer. In this study, gastric cancer showed no significant association with H. pylori. Further studies are warranted to determine the role of other etiological agents such as Epstein-Barr virus, human papillomavirus and possibly environmental and dietetic factors in the occurrence of this pathology.
authors.affiliation: Laboratoire de Microbiologie et Biologie Moléculaire, Equipe micro-organismes et facteurs oncogènes, Faculté de médecine et de Pharmacie de Fès (FMPF), Université Sidi Mohammed Ben Abdellah (USMBA), Fès, Maroc ; Laboratoire de Biotechnologie, Faculté des sciences Dhar el Mehraz, USMBA, Fès, Maroc.
authors.forename: Samia
authors.initials: S
authors.lastname: Alaoui Boukhris
authors.affiliation: Service d'Anatomie pathologique CHU Hassan II, Fès, Maroc ; Laboratoire de Biologie des cancers, FMPF, USMBA, Fès, Maroc.
authors.forename: Afaf
authors.initials: A
authors.lastname: Amarti
authors.affiliation: Laboratoire d'Epidémiologie et de recherche clinique, FMPF, USMBA, Fès, Maroc.
authors.forename: Karima
authors.initials: K
authors.lastname: El Rhazi
authors.affiliation: Laboratoire de Microbiologie et Biologie Moléculaire, Equipe micro-organismes et facteurs oncogènes, Faculté de médecine et de Pharmacie de Fès (FMPF), Université Sidi Mohammed Ben Abdellah (USMBA), Fès, Maroc ; Laboratoire de Biotechnologie, Faculté des sciences Dhar el Mehraz, USMBA, Fès, Maroc.
authors.forename: Mounia
authors.initials: M
authors.lastname: El Khadir
authors.affiliation: Service d'Hépato gastro-entérologie CHU Hassan II de Fès, Equipe Maladies de l'appareil digestif (FMPF), Fès, Maroc ; Laboratoire de Biologie des cancers, FMPF, USMBA, Fès, Maroc.
authors.forename: Dafr-Allah
authors.initials: DA
authors.lastname: Benajah
authors.affiliation: Service d'Hépato gastro-entérologie CHU Hassan II de Fès, Equipe Maladies de l'appareil digestif (FMPF), Fès, Maroc ; Laboratoire de Biologie des cancers, FMPF, USMBA, Fès, Maroc.
authors.forename: Sidi Adil
authors.initials: SA
authors.lastname: Ibrahimi
authors.affiliation: Laboratoire d'Epidémiologie et de recherche clinique, FMPF, USMBA, Fès, Maroc.
authors.forename: Chakib
authors.initials: C
authors.lastname: Nejjari
authors.affiliation: Service de Bactériologie CHU Hassan II, Fès, Maroc ; Laboratoire de Biologie des cancers, FMPF, USMBA, Fès, Maroc.
authors.forename: Mustapha
authors.initials: M
authors.lastname: Mahmoud
authors.affiliation: Laboratoire de Biotechnologie, Faculté des sciences Dhar el Mehraz, USMBA, Fès, Maroc.
authors.forename: Abdellah
authors.initials: A
authors.lastname: Souleimani
authors.affiliation: Laboratoire de Microbiologie et Biologie Moléculaire, Equipe micro-organismes et facteurs oncogènes, Faculté de médecine et de Pharmacie de Fès (FMPF), Université Sidi Mohammed Ben Abdellah (USMBA), Fès, Maroc ; Laboratoire de Biotechnologie, Faculté des sciences Dhar el Mehraz, USMBA, Fès, Maroc ; Laboratoire de Biologie des cancers, FMPF, USMBA, Fès, Maroc.
authors.forename: Bahia
authors.initials: B
authors.lastname: Bennani
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005756:Gastritis / Q000453:epidemiology* / Q000382:microbiology / Q000473:pathology*; D005838:Genotype; D016481:Helicobacter Infections / Q000453:epidemiology* / Q000473:pathology*; D016480:Helicobacter pylori / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009018:Morocco / Q000453:epidemiology; D015995:Prevalence; D012307:Risk Factors