title: The identification and pharmacokinetic studies of metabolites of salvianolic acid B after intravenous administration in rats.
abstract: OBJECTIVE
  To identify and quantify the major metabolites of salvianolic acid B (SAB) after intravenous injection in rats.
  
  
  METHODS
  LC-IT/TOF-MS was used to identify the metabolites in rat bile, plasma, and urine; LC-MS/MS was used to quantify the two major metabolites.
  
  
  RESULTS
  In rat bile, plasma, and urine, nine metabolites were identified, including methylated metabolites of SAB, lithospermic acid (LSA), the decarboxylation and methylation metabolites of LSA, salvianolic acid S (SAS), and dehydrated-SAS. The t1/2 of monomethyl-SAB and LSA were both very short, and monomethyl-SAB had a larger AUC than LSA in rats.
  
  
  CONCLUSIONS
  Nine metabolites were found, the metabolic pathway was described, and the pharmacokinetic profiles of LSA and monomethyl-SAB were studied, thereby clarifying that methylation was the dominant metabolic pathway for SAB in rats.
authors.affiliation: State Key Laboratory of Natural Medicines, Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Qu
authors.initials: Q
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Natural Medicines, Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Hao
authors.affiliation: State Key Laboratory of Natural Medicines, Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Fei-Yan
authors.initials: FY
authors.lastname: Li
authors.affiliation: State Key Laboratory of Natural Medicines, Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Natural Medicines, Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Guang-Ji
authors.initials: GJ
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Natural Medicines, Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China. Electronic address: hhp_770505@hotmail.com.
authors.forename: Hai-Ping
authors.initials: HP
authors.lastname: Hao
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D001572:Benzofurans / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics*; D001646:Bile / Q000737:chemistry; D008297:Male; D015394:Molecular Structure; D010949:Plasma / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D014556:Urine / Q000737:chemistry; D053630:Depsides
chemical_list: D001572:Benzofurans; C076944:salvianolic acid B; D053630:Depsides
keywords: Identification; Metabolites; Pharmacokinetics; Salvianolic acid B