title: The effect of Chinese medicine Pu-Ren-Dan on pancreatic angiogenesis in high fat diet/streptozotocin-induced diabetic rats.
abstract: OBJECTIVE
  The islet vascular system is critical for β-cell function. This study investigated the antidiabetic effect of the Chinese Pu-Ren-Dan (PRD) recipe by regulating the pancreatic angiogenic factors in T2DM rats.
  
  
  METHODS
  High fat diet/streptozotocin-induced obese type-2 diabetes mellitus rats were developed and treated with PRD for 4 weeks. Then glucolipid metabolism, insulin secretion, pancreatic blood flow, ultrastructure of islet β-cell, histological changes of islet and protein expressions of pancreatic angiogenic factors were investigated.
  
  
  RESULTS
  PRD-reduced T2DM rats' body weight and blood glucose level resisted the lipid metabolism disturbance, and ameliorated the insulin resistance and β-cell function. In addition, the histological and morphological studies proved that PRD could maintain the normal distribution of endocrine cell in islet and normal ultrastructure of β cell. An increased pancreatic blood flow was observed after the PRD treatment. In the investigation of pancreatic angiogenic factors, PRD inhibited the decreased expression of VEGF and Ang-1, and reversed the reduction of VEGFR2 and Tie2 phosphorylation in T2DM rats; the Ang-2 and TGFβ expression were up-regulated by PRD while PKC was activated; endostatin and angiostatin were down-regulated by PRD.
  
  
  CONCLUSIONS
  The results suggest that increasing VEGF expression, regulating VEGF/VEGFR2 signaling, stimulating Ang-1/Tie-2 signaling pathway, and inhibiting PKC-TGFβ signaling and antiangiogenic factors might be the underlying mechanism of PRD's antidiabetic effect.
authors.affiliation: Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, P. R. China.
authors.forename: Binan
authors.initials: B
authors.lastname: Lu
authors.affiliation: Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, P. R. China.
authors.forename: Yongfei
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, P. R. China.
authors.forename: Ziliang
authors.initials: Z
authors.lastname: Du
authors.affiliation: Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, P. R. China.
authors.forename: Shu
authors.initials: S
authors.lastname: Chen
authors.affiliation: Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, P. R. China.
authors.forename: D
authors.initials: D
authors.lastname: Deligema
authors.affiliation: Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, P. R. China.
authors.forename: Zongran
authors.initials: Z
authors.lastname: Pang
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D003924:Diabetes Mellitus, Type 2 / Q000503:physiopathology*; D059305:Diet, High-Fat; D004365:Drugs, Chinese Herbal; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D008297:Male; D009389:Neovascularization, Pathologic / Q000517:prevention & control*; D010179:Pancreas / Q000098:blood supply / Q000187:drug effects*; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D042787:Receptor, TIE-2 / Q000378:metabolism; D013311:Streptozocin; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D007328:Insulin; D013311:Streptozocin; D042787:Receptor, TIE-2; D040301:Vascular Endothelial Growth Factor Receptor-2
keywords: Pancreatic angiogenic factors; traditional Chinese medicine Pu-Ren-Dan; type-2 diabetes mellitus