title: Enzymatic transformation of polydatin to resveratrol by piceid-β-D-glucosidase from Aspergillus oryzae.
abstract: Resveratrol is now gaining much attention because of its pharmacological properties. Polygonum cuspidatum has the highest content of resveratrol in plants and is the best material offering resveratrol. However, the content of resveratrol in P. cuspidatum is much lower compared with its glycoside polydatin. In this study, enzymatic transformation of polydatin to resveratrol by piceid-β-D-glucosidase from Aspergillus oryzae sp. 100 was investigated. The biotransformation conditions were optimized. Under the optimized conditions of 60 °C, pH 5.0, substrate concentration of 40 g/L and piceid-β-D-glucosidase activity of 5 U/mL, enzymatic transformation of polydatin from P. cuspidatum was successfully performed, during which 22.5 g/L of resveratrol was produced after reacting for 4 h, with the substrate conversion rate of 2 g/h/U of piceid-β-D-glucosidase. A feasible and environment friendly process of enzymatic transformation of polydatin to resveratrol was developed, which provides a promising and competitive alternative for the production of resveratrol.
authors.affiliation: College of Bioengineering, Dalian Polytechnic University, Dalian, 116034, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.forename: Dai
authors.initials: D
authors.lastname: Li
authors.forename: Ziqing
authors.initials: Z
authors.lastname: Gao
authors.forename: Chunzhi
authors.initials: C
authors.lastname: Zhang
mesh_terms: D001236:Aspergillus oryzae / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D045468:Fallopia japonica / Q000378:metabolism*; D005960:Glucosides / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D005960:Glucosides; D013267:Stilbenes; D001617:beta-Glucosidase; D000077185:Resveratrol; C058229:polydatin