title: The effect of poly(3-hydroxybutyrate-co-3- hydroxyhexanoate) (PHBHHx) and human mesenchymal stem cell (hMSC) on axonal regeneration in experimental sciatic nerve damage.
abstract: This study is designed to evaluate the treatment effect of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) and human mesenchymal stem cells (hMSC) on axonal regeneration in experimental rat sciatic nerve damage, and compare the results of this modality with autologous nerve grafting. In Spraque-Dawley albino rats, 10-mm-long experimental nerve gaps were created. Three groups were constituted, the gap was repaired with autologous nerve graft (autograft group), PHBHHx nerve graft alone (PHBHHx alone group), and PHBHHx nerve graft with hMSCs inside (PHBHHx with hMSC group), respectively. The results were evaluated with functional recovery, electrophysiological evaluation, and histological evaluation either with light microscopy and transmission electron microscopy for axonal regeneration and myelin formation. In functional evaluation, autograft and PHBHHx with hMSC groups showed functional improvement with time, whereas PHBHHx alone group did not. Electrophysiological evaluation showed better results in autograft and PHBHHx with hMSC groups when compared to PHBHHx alone group. There was no statistical difference between autograft and PHBHHx with hMSC groups. Histological evaluation showed regenerated axons in each group. Autograft group was better than the others, and PHBHHx with hMSC group was better than PHBHHx alone group both for axonal regeneration and myelin formation. This study showed that the nerve grafts which were prepared from PHBHHx with oriented nanofiber three-dimensional surfaces aided to nerve regeneration, either used alone or with hMSC. PHBHHx provided better nerve regeneration when used with hMSCs inside than alone, and reached the same statistical treatment effect in functional evaluation and electrophysiological evaluation when compared to autografting.
authors.affiliation: 1Department of Neurosurgery.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Sakar
authors.forename: Petek
authors.initials: P
authors.lastname: Korkusuz
authors.forename: Murat
authors.initials: M
authors.lastname: Demirbilek
authors.forename: Duygu Uçkan
authors.initials: DU
authors.lastname: Cetinkaya
authors.forename: Sevil
authors.initials: S
authors.lastname: Arslan
authors.forename: Emir Baki
authors.initials: EB
authors.lastname: Denkbaş
authors.forename: Çağrı Mesut
authors.initials: ÇM
authors.lastname: Temuçin
authors.forename: Elif
authors.initials: E
authors.lastname: Bilgiç
authors.forename: Derya Burcu
authors.initials: DB
authors.lastname: Hazer
authors.forename: Gökhan
authors.initials: G
authors.lastname: Bozkurt
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000494:pharmacology* / Q000627:therapeutic use*; D000818:Animals; D001369:Axons / Q000473:pathology / Q000648:ultrastructure; D002208:Caproates / Q000494:pharmacology* / Q000627:therapeutic use*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004576:Electromyography; D019054:Evoked Potentials, Motor / Q000187:drug effects; D005260:Female; D006801:Humans; D008124:Locomotion / Q000187:drug effects / Q000502:physiology; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000502:physiology / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D018482:Muscle, Skeletal / Q000473:pathology / Q000503:physiopathology; D009413:Nerve Fibers, Myelinated / Q000473:pathology / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020426:Sciatic Neuropathy / Q000188:drug therapy* / Q000601:surgery*; D000094122:Polyhydroxybutyrates
chemical_list: D020155:3-Hydroxybutyric Acid; D002208:Caproates; C115940:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates
keywords: PHBHHx; bacterial polyester; bone marrow stromal cell; hMSC; peripheral nerve; stem cell