title: Conventional specimen preparation techniques for transmission electron microscopy of cultured cells.
abstract: In this chapter, we discuss conventional methods for handling cells grown suspended in liquid culture and on solid substrates. Protocols are given on how to prepare cultures for transmission electron microscopy, including the most commonly used buffers, fixatives, enrobement media, and embedding resins. These methods are suitable for a wide variety of organisms, ranging from prokaryotic bacteria to mammalian cells.
authors.affiliation: IMAGE Center, Southern Illinois University, Carbondale, IL, USA.
authors.forename: John J
authors.initials: JJ
authors.lastname: Bozzola
mesh_terms: D001419:Bacteria; D002478:Cells, Cultured; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D013048:Specimen Handling / Q000379:methods*