title: [Relationship between mRNA expression of MnSOD and manganese neurotoxicity].
abstract: OBJECTIVE
  To investigate the relationship between mRNA expression of manganese superoxide dismutase (MnSOD) and manganese neurotoxicity.
  
  
  METHODS
  Thirty-one patients with occupational chronic manganese poisoning (case group), as well as 31 controls exposed to the same condition (control group), were included in the study. Whole blood RNA was extracted, and the mRNA expression of MnSOD was measured by RT-PCR; the two groups were compared in terms of the mRNA expression of MnSOD. PC12 cells were treated with 0, 100, 200, 400, 600, 800, and 1000 ümol/L MnCl₂ for l, 2, 3, and 4 d; the cell viability was determined by MTT assay, and the mRNA expression of MnSOD was measured by RT-PCR.
  
  
  RESULTS
  The case group had significantly lower mRNA expression of MnSOD than the control group (0.390 ± 0.080 vs 0.582 ± 0.219, P < 0.05). MnCl2 had a toxic effect on PC12 cells; the concentration of MnCl₂ was positively correlated with the toxic effect but negatively correlated with the mRNA expression of MnSOD.
  
  
  CONCLUSIONS
  MnSOD mRNA may be involved in the manganese-induced damage of nerve cells. It is hypothesized that high mRNA expression of MnSOD may play an inhibitory effect on manganese neurotoxicity.
authors.affiliation: Shandong Academy of Occupational Health and Occupational Medicine 250002, China.
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Fan
authors.forename: Yong-jian
authors.initials: YJ
authors.lastname: Yan
authors.forename: Shao-lei
authors.initials: SL
authors.lastname: Cai
authors.forename: Yue-ling
authors.initials: YL
authors.lastname: He
authors.forename: Ming-gang
authors.initials: MG
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000818:Animals; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D020149:Manganese Poisoning / Q000235:genetics*; D008875:Middle Aged; D020258:Neurotoxicity Syndromes / Q000235:genetics*; D016716:PC12 Cells; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013482:Superoxide Dismutase / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D013482:Superoxide Dismutase