title: Isogenic radiation resistant cell lines: development and validation strategies.
abstract: OBJECTIVE
  The comparison of cell lines with differing radiosensitivities and their molecular response to radiation exposure has been used in a number of human cancer models to study the molecular response to radiation. This review proposes to analyze and compare the protocols used by investigators for the development and validation of these isogenic models of radioresistance.
  
  
  CONCLUSIONS
  There is large variability in the strategies used to generate and validate isogenic models of radioresistance. Further characterization of these models is required.
authors.affiliation: Radiation and Urologic Oncology, Applied Radiation Therapy Trinity and Prostate Molecular Oncology Research Group, Discipline of Radiation Therapy, Trinity College Dublin , Ireland.
authors.forename: Niamh
authors.initials: N
authors.lastname: McDermott
authors.forename: Armelle
authors.initials: A
authors.lastname: Meunier
authors.forename: Thomas H
authors.initials: TH
authors.lastname: Lynch
authors.forename: Donal
authors.initials: D
authors.lastname: Hollywood
authors.forename: Laure
authors.initials: L
authors.lastname: Marignol
mesh_terms: D017209:Apoptosis / Q000528:radiation effects; D002453:Cell Cycle / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D004249:DNA Damage; D006801:Humans; D009369:Neoplasms / Q000532:radiotherapy*; D018384:Oxidative Stress; D011829:Radiation Dosage; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured