title: Selection of osteoprogenitors from the jaw periosteum by a specific animal-free culture medium.
abstract: The goal of our research work is to establish mesenchymal osteoprogenitors derived from human jaw periosteum for tissue engineering applications in oral and maxillofacial surgery. For future autologous and/or allogeneic transplantations, some issues must be addressed. On the one hand, animal-free culture conditions have yet to be established. On the other hand, attempts should be undertaken to shorten the in vitro culturing process efficiently. The aim of the present study is to compare and analyze the phenotype of osteoprogenitors from the jaw periosteum under normal FCS-containing and animal-free culture conditions. Therefore, we analyzed the proliferation rates of MesenCult-XF medium (MC-) in comparison to DMEM-cultured JPCs. Whereas jaw periosteal cells (JPCs) show relatively slow proliferation rates and a fibroblastoid shape under DMEM culture conditions, MC-cultured JPCs diminished their cell size significantly and proliferated rapidly. By live-monitoring measurements of adhesion and proliferation, we made an interesting observation: whereas the proliferation of the MSCA-1(+) subpopulation and the unseparated cell fraction were favored by the animal-free culture medium, the proliferation of the MSCA-1(-) subpopulation seemed to be repressed under these conditions. The alkaline phosphatase expression pattern showed similar results under both culture conditions. Comparison of the mineralization capacity revealed an earlier formation of calcium-phosphate precipitates under MC culture conditions; however, the mineralization capacity of the DMEM-cultured cells seemed to be higher. We conclude that the tested animal-free medium is suitable for the in vitro expansion and even for the specific selection of osteoprogenitor cells derived from the jaw periosteum.
authors.affiliation: Department of Oral and Maxillofacial Surgery, University Hospital of Tübingen, Tübingen, Germany.
authors.forename: Dorothea
authors.initials: D
authors.lastname: Alexander
authors.affiliation: Department of Oral and Maxillofacial Surgery, University Hospital of Tübingen, Tübingen, Germany.
authors.forename: Melanie
authors.initials: M
authors.lastname: Rieger
authors.affiliation: Department of Conservative Dentistry, University Hospital of Tübingen, Tübingen, Germany ; Dental practice Zahngesundheit Waiblingen, Waiblingen, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Klein
authors.affiliation: Department of Oral and Maxillofacial Surgery, University Hospital of Tübingen, Tübingen, Germany.
authors.forename: Nina
authors.initials: N
authors.lastname: Ardjomandi
authors.affiliation: Department of Oral and Maxillofacial Surgery, University Hospital of Tübingen, Tübingen, Germany.
authors.forename: Siegmar
authors.initials: S
authors.lastname: Reinert
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D003470:Culture Media / Q000737:chemistry*; D015870:Gene Expression; D006801:Humans; D007568:Jaw / Q000166:cytology* / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010011:Osteocytes / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis; D010521:Periosteum / Q000166:cytology* / Q000378:metabolism; D061251:Primary Cell Culture
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers; D003470:Culture Media; C540400:W8B2 protein, human; D000469:Alkaline Phosphatase