title: Role of StdA in adhesion of Salmonella enterica serovar Enteritidis phage type 8 to host intestinal epithelial cells.
abstract: BACKGROUND
  Salmonella is often implicated in foodborne outbreaks, and is a major public health concern in the United States and throughout the world. Salmonella enterica serovar Enteritidis (SE) infection in humans is often associated with the consumption of contaminated poultry products. Adhesion to epithelial cells in the intestinal mucosa is a major pathogenic mechanism of Salmonella in poultry. Transposon mutagenesis identified stdA as a potential adhesion mutant of SE. Therefore, we hypothesize StdA plays a significant role in adhesion of SE to the intestinal mucosa of poultry.
  
  
  RESULTS
  To test our hypothesis, we created a mutant of SE in which stdA was deleted. Growth and motility were assayed along with the in vitro and in vivo adhesion ability of the ∆stdA when compared to the wild-type SE strain. Our data showed a significant decrease in motility in ∆stdA when compared to the wild-type and complemented strain. A decrease in adhesion to intestinal epithelial cells as well as in the small intestine and cecum of poultry was observed in ∆stdA. Furthermore, the lack of adhesion correlated to a defect in invasion as shown by a cell culture model using intestinal epithelial cells and bacterial recovery from the livers and spleens of chickens.
  
  
  CONCLUSIONS
  These studies suggest StdA is a major contributor to the adhesion of Salmonella to the intestinal mucosa of poultry.
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Shippy
authors.forename: Nicholas M
authors.initials: NM
authors.lastname: Eakley
authors.forename: Dareen M
authors.initials: DM
authors.lastname: Mikheil
authors.affiliation: Department of Animal Sciences, University of Wisconsin-Madison, 1675 Observatory Drive, Madison, WI 53706, USA. fadl@wisc.edu.
authors.forename: Amin A
authors.initials: AA
authors.lastname: Fadl