title: Diagnostic accuracy of anti-alpha-fodrin antibodies for primary Sjögren's syndrome.
abstract: OBJECTIVE
  To estimate the diagnostic accuracy of anti-alpha-fodrin antibodies for primary Sjögren's syndrome (pSS).
  
  
  METHODS
  Sixty-four pSS subjects and 108 non-pSS patients were prospectively enrolled in this study. Serum anti-alpha-fodrin IgA and IgG were detected by ELISA in a blind fashion. The diagnostic accuracy of anti-alpha-fodrin antibodies was assessed by receiver operating characteristic (ROC) curve analysis. Logistic regression was used to investigate whether anti-alpha-fodrin antibodies could improve the accuracy of pSS diagnosis if used in addition to anti-SSA and anti-SSB.
  
  
  RESULTS
  The areas under the ROC curves for anti-alpha-fodrin IgG and IgA were 0.69 (95% confidence interval (CI): 0.60-0.77) and 0.63 (95% CI: 0.54-0.72), respectively (P < 0.01 for both). The optimal diagnostic thresholds for anti-fodrin IgG and IgA were 11.75 U/ml and 9.75 U/ml, respectively, with a sensitivity of 0.59 and 0.55, and a specificity of 0.75 and 0.73, respectively. Anti-alpha-fodrin IgG and IgA antibodies were associated with pSS after adjustment for anti-SSA and anti-SSB.
  
  
  CONCLUSIONS
  Anti-alpha-fodrin IgG and IgA antibodies are useful diagnostic markers which may improve the accuracy of pSS diagnosis.
authors.affiliation: Department of Transfusion, Huashan Hospital, Fudan University , Shanghai , P. R. China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Qin
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Huai-Zhou
authors.initials: HZ
authors.lastname: Wang
authors.forename: Yuan-Lan
authors.initials: YL
authors.lastname: Huang
authors.forename: Hu
authors.initials: H
authors.lastname: Li
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Zhang
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Zhang
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: He
authors.forename: Rong
authors.initials: R
authors.lastname: Xia
authors.forename: Dong-Bao
authors.initials: DB
authors.lastname: Zhao
authors.forename: An-Mei
authors.initials: AM
authors.lastname: Deng
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D002352:Carrier Proteins / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D008840:Microfilament Proteins / Q000276:immunology*; D008875:Middle Aged; D012680:Sensitivity and Specificity; D012859:Sjogren's Syndrome / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D013049:Spectrin
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D002352:Carrier Proteins; D008840:Microfilament Proteins; D013049:Spectrin
keywords: Anti-alpha-fodrin antibody; Diagnosis; Primary Sjögren's syndrome; Sensitivity; Specificity