title: Rab11 facilitates cross-talk between autophagy and endosomal pathway through regulation of Hook localization.
abstract: During autophagy, double-membrane autophagosomes deliver sequestered cytoplasmic content to late endosomes and lysosomes for degradation. The molecular mechanism of autophagosome maturation is still poorly characterized. The small GTPase Rab11 regulates endosomal traffic and is thought to function at the level of recycling endosomes. We show that loss of Rab11 leads to accumulation of autophagosomes and late endosomes in Drosophila melanogaster. Rab11 translocates from recycling endosomes to autophagosomes in response to autophagy induction and physically interacts with Hook, a negative regulator of endosome maturation. Hook anchors endosomes to microtubules, and we show that Rab11 facilitates the fusion of endosomes and autophagosomes by removing Hook from mature late endosomes and inhibiting its homodimerization. Thus induction of autophagy appears to promote autophagic flux by increased convergence with the endosomal pathway.
authors.affiliation: Department of Anatomy, Cell, and Developmental Biology, Eötvös Loránd University, Budapest 1117, Hungary MTA-ELTE Comparative Ethology Research Group, Budapest 1117, Hungary Department of Germline Development, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Szatmári
authors.forename: Viktor
authors.initials: V
authors.lastname: Kis
authors.forename: Mónika
authors.initials: M
authors.lastname: Lippai
authors.forename: Krisztina
authors.initials: K
authors.lastname: Hegedus
authors.forename: Tamás
authors.initials: T
authors.lastname: Faragó
authors.forename: Péter
authors.initials: P
authors.lastname: Lorincz
authors.forename: Tsubasa
authors.initials: T
authors.lastname: Tanaka
authors.forename: Gábor
authors.initials: G
authors.lastname: Juhász
authors.forename: Miklós
authors.initials: M
authors.lastname: Sass
mesh_terms: D000818:Animals; D001343:Autophagy / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism* / Q000648:ultrastructure; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005786:Gene Expression Regulation; D008247:Lysosomes / Q000378:metabolism* / Q000648:ultrastructure; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure; D011485:Protein Binding; D055503:Protein Multimerization; D021381:Protein Transport; D015398:Signal Transduction; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D000099193:rab11 GTP-Binding Proteins
chemical_list: D029721:Drosophila Proteins; D033921:Vesicular Transport Proteins; D020691:rab GTP-Binding Proteins; C100411:hook protein, Drosophila; C559123:Rab11 protein, Drosophila; D000099193:rab11 GTP-Binding Proteins