title: [Three-dimensional cell cultures. Applications in basic science and biotechnology].
abstract: The tissue culture technique is widely used in biochemical and molecular studies, offering accessibility to biological material, high reproducibility of results and high throughput format, comparing with organ cultures. However, traditional, two-dimensional cultures (2D cultures) poorly represent the microenvironment of a tissue, and they are gradually replaced with 3D cultures, that enable formation of intercellular contacts, signaling pathways and gene expression characteristic for tissue in vivo. This paper presents the biology of three-dimensional cultures (spheroids), their applications in basic science and biotechnology and methods of spheroids formation.
authors.affiliation: Department of Organic Chemistry, Bioorganic Chemistry and Biotechnology, Faculty of Chemistry, Silesian Technical University, 4 Krzywoustego St., 44-100 Gliwice, Poland.
authors.forename: Radosław
authors.initials: R
authors.lastname: Kitel
authors.affiliation: Department of Organic Chemistry, Bioorganic Chemistry and Biotechnology, Faculty of Chemistry, Silesian Technical University, 4 Krzywoustego St., 44-100 Gliwice, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Czarnecka
authors.affiliation: Center for Translational Research and Molecular Biology of Cancer, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Branch Gliwice, ul. Wybrzeze AK 15, 44-100 Gliwice, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Rusin
mesh_terms: D000818:Animals; D001709:Biotechnology / Q000379:methods; D018929:Cell Culture Techniques / Q000379:methods*; D006801:Humans; D009924:Organ Culture Techniques; D018874:Spheroids, Cellular / Q000166:cytology*