title: [Roles of NKG2D in cytokine-induced killer (CIK) against hematological malignant cells lines].
abstract: This study was purposed to investigate the CIK cell cytotoxicity to hematological malignant cell lines by interaction NKG2D receptors and corresponding ligands. The CIK cells was expanded from healthy individual with interferon (IFN)γ, CD3 monoclonal antibodies (mAb) and interleukin-2 (IL-2). The subset of lymphocyte and the expression of NK cell receptors on CIK cells was detected by flow cytometry; NKG2D ligand expression on hematological malignant cell lines was also analyzed by flow cytometry, the calcein acetoxymethyl ester (CAM) was used for labeling target cells, then the cytotoxicity of CIK cells to hematological malignant cell lines was detected by flow cytometry. The results showed that most of CIK cells expressed CD3 (97.85 ± 1.95%) , CD3(+)CD8(+) cells and CD3(+)CD56(+) cells increased significantly as compared with un-cultured cells (P < 0.001;P = 0.033). About 86% CIK cells expressed NKG2D receptor but no other NK receptors such as CD158a, CD158b and NCR. Different levels of NKG2D ligands were detected in hematological malignant cell lines U266, K562 and Daudi. CIK cells showed high cytotoxicity to these three different cell lines, and this cytotoxicity was partially blocked by treating CIK cells with anti-NKG2D antibody (U266 52.67 ± 4.63% vs 32.67 ± 4.81%, P = 0.008;K562 71.67 ± 4.91% vs 50.33 ± 4.91%, P = 0.007;Daudi 68.67 ± 5.04 vs 52.67 ± 2.60%, P = 0.024) . It is concluded that most of CIK cells express NKG2D receptor, interaction of NKG2D-NKG2D ligands may be one of the mechanisms, by which CIK cells kill hematological malignant cells.
authors.affiliation: Department of Hematology, Changzhou Mumicipal People's Hospital, Changzhou 213003,Jiangsu Province, China.
authors.forename: Jin-Yuan
authors.initials: JY
authors.lastname: He
authors.affiliation: Central Laboratory, Changzhou Mumicipal People's Hospital, Changzhou 213003,Jiangsu Province, China.
authors.forename: Zhu-Xia
authors.initials: ZX
authors.lastname: Jia
authors.affiliation: Department of Hematology, Zhongda Hospital Affiliated to Southeast University, Nanjing 210009, Jiansu Province, China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Cai
authors.affiliation: Department of Hematology, Changzhou Mumicipal People's Hospital, Changzhou 213003,Jiangsu Province, China.
authors.forename: Wen-Min
authors.initials: WM
authors.lastname: Han
authors.affiliation: Department of Hematology, Changzhou Mumicipal People's Hospital, Changzhou 213003,Jiangsu Province, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Xiao
authors.affiliation: Central Laboratory, Changzhou Mumicipal People's Hospital, Changzhou 213003,Jiangsu Province, China.
authors.forename: Ling-Di
authors.initials: LD
authors.lastname: Ma
authors.affiliation: Department of Hematology, Zhongda Hospital Affiliated to Southeast University, Nanjing 210009, Jiansu Province, China. E-mail: luxuzhang2008@163.com.
authors.forename: Xu-Zhang
authors.initials: XZ
authors.lastname: Lu
authors.affiliation: Department of Hematology, Changzhou Mumicipal People's Hospital, Changzhou 213003,Jiangsu Province, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Hematology, Zhongda Hospital Affiliated to Southeast University, Nanjing 210009, Jiansu Province, China.
authors.forename: Bao-An
authors.initials: BA
authors.lastname: Chen
mesh_terms: D000911:Antibodies, Monoclonal / Q000494:pharmacology; D045744:Cell Line, Tumor; D003470:Culture Media / Q000737:chemistry; D055612:Cytokine-Induced Killer Cells / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D008024:Ligands; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D055655:NK Cell Lectin-Like Receptor Subfamily K / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D003470:Culture Media; D007376:Interleukin-2; C527085:KLRK1 protein, human; D008024:Ligands; D055655:NK Cell Lectin-Like Receptor Subfamily K; D007371:Interferon-gamma