title: Non-invasive bioluminescence imaging to monitor the immunological control of a plasmablastic lymphoma-like B cell neoplasia after hematopoietic cell transplantation.
abstract: To promote cancer research and to develop innovative therapies, refined pre-clinical mouse tumor models that mimic the actual disease in humans are of dire need. A number of neoplasms along the B cell lineage are commonly initiated by a translocation recombining c-myc with the immunoglobulin heavy-chain gene locus. The translocation is modeled in the C.129S1-Igha(tm1(Myc)Janz)/J mouse which has been previously engineered to express c-myc under the control of the endogenous IgH promoter. This transgenic mouse exhibits B cell hyperplasia and develops diverse B cell tumors. We have isolated tumor cells from the spleen of a C.129S1-Igha(tm1(Myc)Janz)/J mouse that spontaneously developed a plasmablastic lymphoma-like disease. These cells were cultured, transduced to express eGFP and firefly luciferase, and gave rise to a highly aggressive, transplantable B cell lymphoma cell line, termed IM380. This model bears several advantages over other models as it is genetically induced and mimics the translocation that is detectable in a number of human B cell lymphomas. The growth of the tumor cells, their dissemination, and response to treatment within immunocompetent hosts can be imaged non-invasively in vivo due to their expression of firefly luciferase. IM380 cells are radioresistant in vivo and mice with established tumors can be allogeneically transplanted to analyze graft-versus-tumor effects of transplanted T cells. Allogeneic hematopoietic stem cell transplantation of tumor-bearing mice results in prolonged survival. These traits make the IM380 model very valuable for the study of B cell lymphoma pathophysiology and for the development of innovative cancer therapies.
authors.affiliation: Department of Internal Medicine II, University Hospital Würzburg, Würzburg, Germany ; Center for Interdisciplinary Clinical Research, Würzburg University, Würzburg, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Chopra
authors.affiliation: Department of Internal Medicine II, University Hospital Würzburg, Würzburg, Germany ; Center for Interdisciplinary Clinical Research, Würzburg University, Würzburg, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Kraus
authors.affiliation: Department of Internal Medicine II, University Hospital Würzburg, Würzburg, Germany ; Center for Interdisciplinary Clinical Research, Würzburg University, Würzburg, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Schwinn
authors.affiliation: Department of Internal Medicine II, University Hospital Würzburg, Würzburg, Germany ; Center for Interdisciplinary Clinical Research, Würzburg University, Würzburg, Germany.
authors.forename: Miriam
authors.initials: M
authors.lastname: Ritz
authors.affiliation: Department of Internal Medicine II, University Hospital Würzburg, Würzburg, Germany ; Center for Interdisciplinary Clinical Research, Würzburg University, Würzburg, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Mattenheimer
authors.affiliation: Institute of Pathology, Würzburg University, Würzburg, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Mottok
authors.affiliation: Institute of Pathology, Würzburg University, Würzburg, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Rosenwald
authors.affiliation: Department of Internal Medicine II, University Hospital Würzburg, Würzburg, Germany.
authors.forename: Hermann
authors.initials: H
authors.lastname: Einsele
authors.affiliation: Department of Internal Medicine II, University Hospital Würzburg, Würzburg, Germany ; Center for Interdisciplinary Clinical Research, Würzburg University, Würzburg, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Beilhack
mesh_terms: D000818:Animals; D001402:B-Lymphocytes; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging / Q000379:methods*; D005260:Female; D005434:Flow Cytometry; D018380:Hematopoietic Stem Cell Transplantation; D008163:Luminescent Measurements / Q000379:methods*; D016393:Lymphoma, B-Cell / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D014184:Transplantation, Homologous