title: The association between Factor V Leiden with the presence and severity of coronary artery disease.
abstract: OBJECTIVE
  The presence of Factor V Leiden (FVL) is proposed to be associated with a higher risk for arterial thrombosis. The aim of this study was to examine a relationship between FVL with the presence and severity of angiographically determined coronary artery disease (CAD).
  
  
  METHODS
  In this case-control study, 1083 patients having angiographic evidence of atherosclerosis with ≥50% luminal stenosis in their epicardial coronary tree were compared with patients with no luminal stenosis (n=320) or with luminal stenosis <50% (n=191) at coronary angiography as reference group. The severity of CAD was determined by vessel score and also a semi-quantitative scoring system (Gensini score). The presence of Factor V polymorphisms was analyzed using polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP).
  
  
  RESULTS
  FVL was found to be independently associated with the occurrence of CAD (p=0.020). As compared to wild genotype, heterozygote or homozygote mutant genotypes were more likely associated with a trend towards more severe CAD (adjusted OR=1.85, 95% CI=1.26 to 2.72; p=0.002, and adjusted OR=3.70, 95% CI=1.71 to 8.00; p=0.001; respectively). In addition, the median and inter-quartile range for Gensini score were significantly different among the GG (27.8, 3 to 66.5), GA (53.5, 10 to 104.1), and AA (92.8, 48.1 to 125.9) genotypes (p<0.001).
  
  
  CONCLUSIONS
  Our results confirmed the hypothesis that FVL mutation is a significant determinant of CAD risk. Furthermore, we observed that FVL is independently associated with increasing CAD severity.
authors.affiliation: Department of Surgical and Clinical Pathology, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammadali
authors.initials: M
authors.lastname: Boroumand
authors.affiliation: Department of Laboratory Medicine and Pathology, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Pourgholi
authors.affiliation: Department of Laboratory Medicine and Pathology, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Shayan
authors.initials: S
authors.lastname: Ziaee
authors.affiliation: Department of Surgical and Clinical Pathology, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Maryam Sotoudeh
authors.initials: MS
authors.lastname: Anvari
authors.affiliation: Department of Cardiac Research, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Arash
authors.initials: A
authors.lastname: Jalali
authors.affiliation: Department of Cardiac Research, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: h-goodarzynejad@razi.tums.ac.ir.
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Goodarzynejad
mesh_terms: D003324:Coronary Artery Disease / Q000235:genetics*; D005165:Factor V / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D016017:Odds Ratio; D012720:Severity of Illness Index
chemical_list: C095381:factor V Leiden; D005165:Factor V
keywords: Coronary angiography; Coronary artery disease; Factor V Leiden; G1691A variant