title: Interaction of a CD44+ head and neck squamous cell carcinoma cell line with a stromal cell-derived factor-1-expressing supportive niche: Anin vitromodel.
abstract: The cancer stem cell (CSC) theory implies that CSCs are surrounded by supportive stromal cells, which are known as the CSC niche. Stromal cell-derived factor-1 (SDF-1) shows a multitude of functional effects in head and neck squamous cell carcinoma (HNSCC) cells, including migration and polarization. Therefore, the SDF-1-CXCR4 axis may be involved in the pathophysiology of the progression, recurrence and metastasis of malignant diseases of the head and neck. In the present study, the CD44+HNSCC UM-SCC-11A cell line was used as a model for CSCs. The interaction between the UM-SCC-11A cells and the supportive microenvironmental cells, including fibrocytes, human umbilical vein endothelial cells (HUVECs) and human microvascular vein endothelial cells (HMVECs) was evaluated. All the cell types that were tested were shown to secrete different concentrations of SDF-1 into the surrounding culture medium [mean (m)fibro, 1243.3±156.2 pg/ml; mHMVEC, 1061.4±23.2 pg/ml; mHUVEC, 849.6±110.9 pg/ml]. The migration of the UM-SCC-11A cells towards the supportive cells was increased by a higher supply of SDF-1 (contrfibro, 315.23±61.55 μm; mfibro, 477.73±143.7 μm; Pfibro=0.003; contrHMVEC, 123.41±66.68 μm; mHMVEC, 249.04±111.95 μm; PHMVEC=0.004; contrHUVEC, 189.7±93.26 μm; mHUVEC, 260.82±161.58 μm). The amount of the UM-SCC-11A cells that migrated towards the differentiated fibrocytes was significantly higher than that which migrated towards the HMVECs or HUVECs (Pfibro/HMVEC=2.12E-11; Pfibro/HUVEC=2.28E-5). Cell-cell interaction by podia formation of the UM-SCC-11A cells was observed in all the supportive cell types that were tested. Broadly based cell-cell contacts were observed. By contrast, digitiform podia formations presented by the UM-SCC-11A cells were determined using fluorescence microscopy. The SDF-1-CXCR4 axis is postulated to be a crucial pathway in the interaction between CSCs and their surrounding supportive cells. Understanding the cell-cell interactions in the CSC niche usingin vitromodels may aid in gaining further insight into these mechanisms and finding new strategies of therapy in this field.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Centre Mannheim, Mannheim D-68167, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Faber
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Centre Mannheim, Mannheim D-68167, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Aderhold
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Centre Mannheim, Mannheim D-68167, Germany.
authors.forename: Ulrich Reinhart
authors.initials: UR
authors.lastname: Goessler
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Centre Mannheim, Mannheim D-68167, Germany.
authors.forename: Karl
authors.initials: K
authors.lastname: Hoermann
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Centre Mannheim, Mannheim D-68167, Germany.
authors.forename: Johannes David
authors.initials: JD
authors.lastname: Schultz
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Centre Mannheim, Mannheim D-68167, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Umbreit
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Centre Mannheim, Mannheim D-68167, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Walliczek
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Centre Mannheim, Mannheim D-68167, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Stern-Straeter
keywords: CXCR4; SDF-1; cancer stem cells; head and neck squamous cell carcinoma; stem cell niche