title: Absorption, metabolism, and effects at transcriptome level of a standardized French oak wood extract, Robuvit, in healthy volunteers: pilot study.
abstract: The consumption of wine and spirits, traditionally aged in oak barrels, exposes humans to roburin ingestion. These molecules belong to a class of ellagitannins (ETs), and their only known source is oak wood. Very little is currently known about roburin bioavailability and biological activity. We reported for the first time human absorption of roburins from a French oak wood (Quercus robur) water extract (Robuvit) by measuring the increase of total phenols (from 0.63 ± 0.06 to 1.26 ± 0.18 μg GAE equiv/mL plasma) and the appearance of roburin metabolites (three different glucoronidate urolithins and ellagic acid), in plasma, after 5 days of supplementation. Robuvit supplementation induced also the increase of plasma antioxidant capacity from 1.8 ± 0.05 to 1.9 ± 0.01 nmol Trolox equiv/mL plasma. Moreover, utilizing a combined ex vivo cell culture approach, we assessed the effect of Q. robur metabolites (present in human serum after supplementation) on gene expression modulation, utilizing an Affymetrix array matrix, in endothelial, neuronal, and keratinocyte cell lines. The functional analysis reveals that Robuvit metabolites affect ribosome, cell cycle, and spliceosome pathways.
authors.affiliation: Consiglio per la Ricerca e Sperimentazione in Agricoltura, Food and Nutrition Research Centre , via Ardeatina 546, 00178 Roma, Italy.
authors.forename: Fausta
authors.initials: F
authors.lastname: Natella
authors.forename: Guido
authors.initials: G
authors.lastname: Leoni
authors.forename: Mariateresa
authors.initials: M
authors.lastname: Maldini
authors.forename: Lucia
authors.initials: L
authors.lastname: Natarelli
authors.forename: Raffaella
authors.initials: R
authors.lastname: Comitato
authors.forename: Frank
authors.initials: F
authors.lastname: Schonlau
authors.forename: Fabio
authors.initials: F
authors.lastname: Virgili
authors.forename: Raffaella
authors.initials: R
authors.lastname: Canali
mesh_terms: D000975:Antioxidants / Q000032:analysis; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D003374:Coumarins / Q000097:blood; D019587:Dietary Supplements; D004610:Ellagic Acid / Q000097:blood; D005602:France; D005786:Gene Expression Regulation / Q000187:drug effects; D020719:Glucuronides / Q000097:blood; D006801:Humans; D047348:Hydrolyzable Tannins / Q000378:metabolism / Q000493:pharmacokinetics* / Q000494:pharmacology; D010636:Phenols / Q000097:blood; D010865:Pilot Projects; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics* / Q000494:pharmacology; D029963:Quercus / Q000737:chemistry*; D012270:Ribosomes / Q000187:drug effects / Q000235:genetics; D017381:Spliceosomes / Q000187:drug effects / Q000235:genetics; D059467:Transcriptome
chemical_list: C576587:3,8-dihydroxy-6H-dibenzo(b,d)pyran-6-one glucuronide; D000975:Antioxidants; D003374:Coumarins; D020719:Glucuronides; D047348:Hydrolyzable Tannins; D010636:Phenols; D010936:Plant Extracts; C588251:Robuvit; D004610:Ellagic Acid
keywords: Quercus robur; ellagitannins; gene array; human study; roburins