title: Comparison and phylogenetic analysis based on the B2L gene of orf virus from goats and sheep in China during 2009-2011.
abstract: As a zoonotic infectious disease, orf outbreaks have been reported in China in recent years. However, molecular epidemiology analysis has not been performed for Chinese orf virus (ORFV) strains. Here, we have identified 13 ORFVs from goats and sheep in China between 2009 and 2011. Thirty-four complete B2L sequences were used to construct a phylogenetic tree to elucidate the molecular epidemiology of ORFV in China. Nucleotide sequences of B2L genes of clinical samples and attenuated vaccine strains were aligned and compared. Three genotypes were found by molecular epidemiology analysis. Amino acid substitutions were dispersed among B2 polypeptides from wild and attenuated ORFV strains.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping No. 1 Yanchangpu, Lanzhou, 730046, Gansu, People's Republic of China, zks009@126.com.
authors.forename: Keshan
authors.initials: K
authors.lastname: Zhang
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Liu
authors.forename: Hanjin
authors.initials: H
authors.lastname: Kong
authors.forename: Youjun
authors.initials: Y
authors.lastname: Shang
authors.forename: Xiangtao
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D002681:China; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004474:Ecthyma, Contagious / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D006041:Goats; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009923:Orf virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012756:Sheep; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins