title: Physicochemical factors differentially affect the biomass and bacteriocin production by bovine Enterococcus mundtii CRL1656.
abstract: Bovine Enterococcus mundtii CRL1656 (Centro de Referencia para Lactobacilos Culture Collection) produces an anti-Listeria and anti-Streptococcus dysgalactiae bacteriocin identified as mundticin CRL1656. The strain and its bacteriocin are candidates to be included in a beneficial product to prevent bovine mastitis as an alternative to antimicrobial agents. To optimize the production of biomass and mundticin CRL1656 by E. mundtii CRL1656, a complete 3 × 2(4) factorial design was applied. The effect of culture medium, initial pH, inoculum size, incubation temperature, and agitation conditions on biomass and bacteriocin production was evaluated simultaneously. Growth parameters were determined using the modified Gompertz model. A nonlinear model was used to estimate the effects of the variables on growth parameters. Bacteriocin production was analyzed using a linear mixed model. Optimal biomass and mundticin CRL1656 production by E. mundtii CRL1656 were obtained in different conditions. Maximal growth was recorded in autolyzed yeast, peptone, tryptone, Tween 80, and glucose or M17 broths, pH 6.5, 5.0% inoculum, 30 °C, with agitation. However, bacteriocin titers were higher in autolyzed yeast, peptone, tryptone, Tween 80, and glucose or de Man-Rogosa-Sharpe (MRS) broths, pH 6.5, 30°C, both with or without agitation. Knowledge of the optimum conditions for growth and bacteriocin production of E. mundtii CRL1656 will allow the obtainment of high levels of biomass and mundticin CRL1656 as bioingredients of potential products to prevent bovine mastitis.
authors.affiliation: Centro de Referencia para Lactobacilos, Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina, 4000 San Miguel de Tucumán, Argentina.
authors.forename: M Carolina
authors.initials: MC
authors.lastname: Espeche
authors.affiliation: Centro de Referencia para Lactobacilos, Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina, 4000 San Miguel de Tucumán, Argentina.
authors.forename: M Silvina
authors.initials: MS
authors.lastname: Juárez Tomás
authors.affiliation: Institut für Biometrie, Medizinische Hochschule, 30625 Hannover, Germany.
authors.forename: Birgitt
authors.initials: B
authors.lastname: Wiese
authors.affiliation: Centro de Referencia para Lactobacilos, Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina, 4000 San Miguel de Tucumán, Argentina.
authors.forename: Elena
authors.initials: E
authors.lastname: Bru
authors.affiliation: Centro de Referencia para Lactobacilos, Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina, 4000 San Miguel de Tucumán, Argentina. Electronic address: fnader@cerela.org.ar.
authors.forename: M E Fátima
authors.initials: ME
authors.lastname: Nader-Macías
mesh_terms: D000818:Animals; D001430:Bacteriocins / Q000378:metabolism*; D018533:Biomass; D002417:Cattle / Q000382:microbiology* / Q000502:physiology; D003470:Culture Media / Q000737:chemistry*; D016983:Enterococcus / Q000502:physiology*; D005260:Female; D019936:Probiotics / Q000737:chemistry
chemical_list: D001430:Bacteriocins; D003470:Culture Media; C114588:mundticin
keywords: Enterococcus mundtii; bovine mastitis prevention; lactic acid bacterium; mundticin production