title: High-throughput nonlinear optical microscopy.
abstract: High-resolution microscopy methods based on different nonlinear optical (NLO) contrast mechanisms are finding numerous applications in biology and medicine. While the basic implementations of these microscopy methods are relatively mature, an important direction of continuing technological innovation lies in improving the throughput of these systems. Throughput improvement is expected to be important for studying fast kinetic processes, for enabling clinical diagnosis and treatment, and for extending the field of image informatics. This review will provide an overview of the fundamental limitations on NLO microscopy throughput. We will further cover several important classes of high-throughput NLO microscope designs with discussions on their strengths and weaknesses and their key biomedical applications. Finally, this review will close with a perspective of potential future technological improvements in this field.
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts; Laser Biomedical Research Center, Massachusetts Institute of Technology, Cambridge, Massachusetts; BioSyM Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, Singapore, Singapore. Electronic address: ptso@mit.edu.
authors.forename: Peter T C
authors.initials: PT
authors.lastname: So
authors.affiliation: BioSyM Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, Singapore, Singapore.
authors.forename: Elijah Y S
authors.initials: EY
authors.lastname: Yew
authors.affiliation: Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts; Laser Biomedical Research Center, Massachusetts Institute of Technology, Cambridge, Massachusetts.
authors.forename: Christopher
authors.initials: C
authors.lastname: Rowlands
mesh_terms: D057166:High-Throughput Screening Assays / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D061848:Optical Imaging / Q000379:methods*