title: [Reliability of estrogen receptor, progesterone receptor and human epidermal growth factor receptor 2 expression in breast cancer evaluated by immunohistochemistry].
abstract: OBJECTIVE
  To evaluate the reliability of estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER-2) expression in breast cancer by immunohistochemistry and discuss their influencing factors.
  
  
  METHODS
  The pretreatment biopsy specimens were collected from 148 patients (from Beijing Cancer Hospital between 2004 to 2010) with invasive breast cancer. After reslicing and staining (ER:SP1, PR: 1E2, HER-2: 4B5), the status of ER, PR and HER-2 was analyzed by a pathological expert with automated imaging system Ariol MB-8. And their results were compared with the original reports.
  
  
  RESULTS
  The concordance rates between original reports and automated image system were ER:76.35%, PR:49.32%, HER-2:63.51% while Kappa values 0.12 (P = 0.020) , 0.18 (P = 0.002) and 0.08 (P = 0.200) respectively. And the concordance rates between expert reports and automated image system were ER:93.92%, PR:81.08% and HER-2:76.35% while Kappa values 0.77 (P < 0.001) , 0.67 (P < 0.001) and 0.32 (P < 0.001) respectively.
  
  
  CONCLUSIONS
  For the expressions of ER, PR and HER-2, the disaccording results of original reports and automated image system may be mainly due to the differences of antibodies, staining methods and interpretations.
authors.affiliation: Department of Breast Center, Beijing Cancer Hospital, Beijing 100142, China.
authors.forename: Chong-shan
authors.initials: CS
authors.lastname: Gu
authors.affiliation: Department of Breast Center, Beijing Cancer Hospital, Beijing 100142, China.
authors.forename: Min
authors.initials: M
authors.lastname: Ying
authors.affiliation: Department of Breast Center, Beijing Cancer Hospital, Beijing 100142, China.
authors.forename: Ying-jian
authors.initials: YJ
authors.lastname: He
authors.affiliation: Department of Breast Center, Beijing Cancer Hospital, Beijing 100142, China.
authors.forename: Yi-qiang
authors.initials: YQ
authors.lastname: Liu
authors.affiliation: Department of Breast Center, Beijing Cancer Hospital, Beijing 100142, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Dong
authors.affiliation: Department of Breast Center, Beijing Cancer Hospital, Beijing 100142, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Ouyang
authors.affiliation: Department of Breast Center, Beijing Cancer Hospital, Beijing 100142, China. Email: aiping_lu@sina.com.
authors.forename: Ai-ping
authors.initials: AP
authors.lastname: Lu
mesh_terms: D000368:Aged; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism*; D015203:Reproducibility of Results
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; C508053:ERBB2 protein, human