title: [Pichia pastoris as an expression system for recombinant protein production].
abstract: Pichia pastoris has become increasingly popular as a host for recombinant protein production in recent years. P. pastoris is more cost effective and allows achieving higher expression levels than insect and mammalian cells. It also offers some significant advantages over E. coli expression systems, such as avoiding problems with proper protein folding. Also, P. pastoris as an eukaryotic organism can carry out posttranslational modifications of produced proteins. Additionally, P. pastoris can produce high levels of recombinant proteins in extracellular medium which simplifies protein purification. Having many advantages over other expression systems makes P. pastoris an organism of choice for industrial protein production.
authors.affiliation: Department of Biochemistry, Nicolaus Copernicus University, 1 Lwowska St., 87-100 Toruń, Poland. annaciar@doktorant.umk.pl
authors.forename: Anna
authors.initials: A
authors.lastname: Ciarkowska
authors.affiliation: Department of Biochemistry, Nicolaus Copernicus University, 1 Lwowska St., 87-100 Toruń, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Jakubowska
mesh_terms: D010843:Pichia / Q000235:genetics* / Q000378:metabolism*; D017510:Protein Folding; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D011994:Recombinant Proteins