title: Bio-augmentation as a tool for improving the modified sequencing batch biofilm reactor.
abstract: Biological treatment of domestic sewage was accomplished in a pilot-scale modified sequencing batch biofilm reactor (SBBR) bio-augmented with consortium of 5 strains of indigenous bacteria (genus Pseudomonas and Bacillus). The reactor consisted of fibrous filler in the upper and ceramsite filter media in the bottom. It demonstrated to have a short hydraulic residence time (HRT) for 10 h and good quality effluent to cope with low C/N ratio domestic wastewater. The biofilm attached fibrous fillers mainly contributed to contaminants removal. Bio-augmentation dramatically enhanced the removal efficiency of chemical oxygen demand (COD), total phosphorus (TP), and especially total nitrogen (TN), which increased respectively from 80.3%, 58.1% and 41.3% to 83.7%, 67.8% and 58.7%. Polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) technique indicated the 5 strains' survival in the reactor and that Bacillus cereus strain ZQN2 was the most dominant bacteria.
authors.affiliation: School of Civil Engineering, Wuhan University, Wuhan 430072, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.affiliation: School of Civil Engineering, Wuhan University, Wuhan 430072, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Ji
authors.affiliation: School of Civil Engineering, Wuhan University, Wuhan 430072, China. Electronic address: hywang96@126.com.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Wang
authors.affiliation: Central and Southern China Municipal Engineering Design and Research Institute, Wuhan 430010, China.
authors.forename: Huaiyu
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Civil Engineering and Architecture, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D001409:Bacillus cereus / Q000502:physiology*; D001673:Biodegradation, Environmental; D018441:Biofilms; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors; D005374:Filtration; D009584:Nitrogen / Q000737:chemistry / Q000378:metabolism; D010758:Phosphorus / Q000737:chemistry / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D012722:Sewage / Q000382:microbiology; D014865:Waste Disposal, Fluid / Q000379:methods; D062065:Wastewater / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism; D018508:Water Purification / Q000379:methods
chemical_list: D009584:Nitrogen; D010758:Phosphorus; D012722:Sewage; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Bio-augmentation; Denaturing gradient gel electrophoresis; Domestic sewage; Modified sequencing batch biofilm reactor; Nitrogen removal