title: Construction and fed-batch cultivation of Candida famata with enhanced riboflavin production.
abstract: Riboflavin (vitamin B2) is an essential nutrition component serving as a precursor of coenzymes FMN and FAD that are involved mostly in reactions of oxidative metabolism. Riboflavin is produced in commercial scale and is used in feed and food industries, and in medicine. The yeast Candida famata (Candida flareri) belongs to the group of so called "flavinogenic yeasts" which overproduce riboflavin under iron limitation. Three genes SEF1, RIB1 and RIB7 coding for a putative transcription factor, GTP cyclohydrolase II and riboflavin synthase, respectively were simultaneously overexpressed in the background of a non-reverting riboflavin producing mutant AF-4, obtained earlier in our laboratory using methods of classical selection (Dmytruk et al. (2011), Metabolic Engineering 13, 82-88). Cultivation conditions of the constructed strain were optimized for shake-flasks and bioreactor cultivations. The constructed strain accumulated up to 16.4g/L of riboflavin in optimized medium in a 7L laboratory bioreactor during fed-batch fermentation.
authors.affiliation: Institute of Cell Biology, NAS of Ukraine, Drahomanov Street, 14/16, Lviv 79005, Ukraine.
authors.forename: Kostyantyn
authors.initials: K
authors.lastname: Dmytruk
authors.affiliation: Institute of Cell Biology, NAS of Ukraine, Drahomanov Street, 14/16, Lviv 79005, Ukraine.
authors.forename: Oleksy
authors.initials: O
authors.lastname: Lyzak
authors.affiliation: Institute of Cell Biology, NAS of Ukraine, Drahomanov Street, 14/16, Lviv 79005, Ukraine.
authors.forename: Valentyna
authors.initials: V
authors.lastname: Yatsyshyn
authors.affiliation: University of Rzeszow, Zelwerowicza 4, Rzeszow 35-601, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Kluz
authors.affiliation: University of Rzeszow, Zelwerowicza 4, Rzeszow 35-601, Poland.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Sibirny
authors.affiliation: University of Rzeszow, Zelwerowicza 4, Rzeszow 35-601, Poland.
authors.forename: Czeslaw
authors.initials: C
authors.lastname: Puchalski
authors.affiliation: Institute of Cell Biology, NAS of Ukraine, Drahomanov Street, 14/16, Lviv 79005, Ukraine; University of Rzeszow, Zelwerowicza 4, Rzeszow 35-601, Poland. Electronic address: sibirny@yahoo.com.
authors.forename: Andriy
authors.initials: A
authors.lastname: Sibirny
mesh_terms: D061249:Batch Cell Culture Techniques; D019149:Bioreactors; D002175:Candida / Q000254:growth & development* / Q000378:metabolism*; D005285:Fermentation; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D006136:GTP Cyclohydrolase / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D007501:Iron / Q000378:metabolism*; D060847:Metabolic Engineering; D009154:Mutation; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012256:Riboflavin / Q000096:biosynthesis*; D012258:Riboflavin Synthase / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; D007501:Iron; D012258:Riboflavin Synthase; D006136:GTP Cyclohydrolase; D012256:Riboflavin
keywords: Candida famata; Fermentation; Riboflavin; Yeast