title: Plasma activity of endothelial lipase impacts high-density lipoprotein metabolism and coronary risk factors in humans.
abstract: OBJECTIVE
  Endothelial lipase (EL) is a determinant of plasma levels of high-density lipoprotein cholesterol (HDL-C). However, little is known about the impact of EL activity on plasma lipid profile. We aimed to establish a new method to evaluate EL-specific phospholipase activity in humans.
  
  
  METHODS
  Plasma samples were obtained from 115 patients with coronary artery disease (CAD) and 154 patients without CAD. Plasma EL protein was immunoprecipitated using an anti-EL monoclonal antibody after plasma non-specific immunoglobulins were removed by incubation with ProteinA. The phospholipase activity of the immunoprecipitated samples was measured using a fluorogenic phospholipase substrate, Bis-BODIPY FL C11-PC.
  
  
  RESULTS
  The EL-specific phospholipase assay revealed that plasma EL activity was inversely correlated with HDL-C levels (R = -0.3088, p＜0.0001). In addition, the EL activity was associated with cigarette smoking. Furthermore, EL activity in CAD patients was significantly higher than that in nonCAD patients. Concomitantly, the HDL-C level in CAD patients were significantly lower than that in non-CAD patients.
  
  
  CONCLUSIONS
  We have established a method for human plasma EL-specific phospholipase activity by combination of EL immunoprecipitation and a fluorogenic phospholipid substrate. Plasma EL activity was associated with not only plasma HDL-C levels but also the risks for CAD.
authors.affiliation: Division of Cardiovascular Medicine, Kobe University Graduate School of Medicine.
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Ishida
authors.forename: Kazuya
authors.initials: K
authors.lastname: Miyashita
authors.forename: Noriaki
authors.initials: N
authors.lastname: Kinoshita
authors.forename: Kenta
authors.initials: K
authors.lastname: Mori
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Yasuda
authors.forename: Ryuji
authors.initials: R
authors.lastname: Toh
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Nakajima
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Imamura
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Hirata
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D003324:Coronary Artery Disease / Q000378:metabolism; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D047468:Immunoprecipitation; D008049:Lipase / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000201:enzymology; D010740:Phospholipases / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012307:Risk Factors; D055815:Young Adult
chemical_list: D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D008075:Lipoproteins, HDL; D010743:Phospholipids; D011994:Recombinant Proteins; D010740:Phospholipases; C410382:LIPG protein, human; D008049:Lipase