title: Pickering emulsion stabilized by lipase-containing periodic mesoporous organosilica particles: a robust biocatalyst system for biodiesel production.
abstract: A novel catalytic system of Pickering emulsion stabilized by lipase-containing periodic mesoporous organosilica was constructed (named LP@PE) and used as biocatalyst for biodiesel production. The reaction parameters were optimized and the optimum conditions were as follows: the water fraction 0.65%, molar ratio of ethanol to oleic acid 2:1, immobilized lipase particles 150mg, phosphate buffer pH 7.0 and temperature 30°C. Under these conditions, the maximum biodiesel yield obtained via esterification of oleic acid with ethanol could reach 95.8%. The biodiesel yield could maintain 88.6% after LP@PE was used 15times. The LP@PE was also used in the synthesis of biodiesel from Jatropha curcas oil. The highest yield could reach 87.1% and the yield was 73.0% after 10 cycles. All these results demonstrated that Pickering emulsion system stabilized by immobilized enzyme may possess much potential in many enzymatic industrial applications.
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China.
authors.forename: Xinlong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Biophysics, Hebei University of Technology, Tianjin 300401, China.
authors.forename: Yafei
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China.
authors.forename: Liya
authors.initials: L
authors.lastname: Zhou
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: He
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China. Electronic address: jgao@hebut.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
mesh_terms: D055162:Biocatalysis; D056804:Biofuels; D001709:Biotechnology / Q000379:methods*; D002021:Buffers; D002175:Candida / Q000201:enzymology; D004655:Emulsions; D004951:Esterification; D000431:Ethanol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000378:metabolism*; D019301:Oleic Acid / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D016062:Porosity; D059027:Recycling; D012822:Silicon Dioxide; D013696:Temperature
chemical_list: D056804:Biofuels; D002021:Buffers; D004655:Emulsions; D010710:Phosphates; D019301:Oleic Acid; D000431:Ethanol; D012822:Silicon Dioxide; D008049:Lipase
keywords: Biodiesel; Lipase; Periodic mesoporous organosilica; Pickering emulsion