title: Human slow troponin T (TNNT1) pre-mRNA alternative splicing is an indicator of skeletal muscle response to resistance exercise in older adults.
abstract: Slow skeletal muscle troponin T (TNNT1) pre-messenger RNA alternative splicing (AS) provides transcript diversity and increases the variety of proteins the gene encodes. Here, we identified three major TNNT1 splicing patterns (AS1-3), quantified their expression in the vastus lateralis muscle of older adults, and demonstrated that resistance training modifies their relative abundance; specifically, upregulating AS1 and downregulating AS2 and AS3. In addition, abundance of TNNT1 AS2 correlated negatively with single muscle fiber-specific force after resistance training, while abundance of AS1 correlated negatively with V max. We propose that TNNT1 AS1, AS2 and the AS1/AS2 ratio are potential quantitative biomarkers of skeletal muscle adaptation to resistance training in older adults, and that their profile reflects enhanced single fiber muscle force in the absence of significant increases in fiber cross-sectional area.
authors.affiliation: Section on Gerontology and Geriatric Medicine, Department of Internal Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina.
authors.forename: Tan
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Section on Gerontology and Geriatric Medicine, Department of Internal Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina. Present address: Division of Sports and Health, KyungSung University, Busan, South Korea.
authors.forename: Seung Jun
authors.initials: SJ
authors.lastname: Choi
authors.affiliation: Section on Gerontology and Geriatric Medicine, Department of Internal Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina.
authors.forename: Zhong-Min
authors.initials: ZM
authors.lastname: Wang
authors.affiliation: Section on Gerontology and Geriatric Medicine, Department of Internal Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina.
authors.forename: Alexander
authors.initials: A
authors.lastname: Birbrair
authors.affiliation: Section on Gerontology and Geriatric Medicine, Department of Internal Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina.
authors.forename: María L
authors.initials: ML
authors.lastname: Messi
authors.affiliation: Department of Physiology, Wayne State University School of Medicine.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Jin
authors.affiliation: Department of Health and Exercise Science, Wake Forest University, Winston-Salem, North Carolina.
authors.forename: Anthony P
authors.initials: AP
authors.lastname: Marsh
authors.affiliation: Section on Gerontology and Geriatric Medicine, Department of Internal Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina. J Paul Sticht Center on Aging, Wake Forest School of Medicine, Winston-Salem, North Carolina.
authors.forename: Barbara
authors.initials: B
authors.lastname: Nicklas
authors.affiliation: Section on Gerontology and Geriatric Medicine, Department of Internal Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina. J Paul Sticht Center on Aging, Wake Forest School of Medicine, Winston-Salem, North Carolina. odelbono@wakehealth.edu.
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Delbono
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000235:genetics*; D001706:Biopsy; D017079:Exercise Tolerance / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012326:RNA Splicing / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D020107:Troponin T / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D020107:Troponin T
keywords: Alternative splicing; Resistance exercise.; Skeletal muscle; Troponin T