title: Hyperspectral darkfield microscopy of PEGylated gold nanoparticles targeting CD44-expressing cancer cells.
abstract: We present a new hyperspectral darkfield imaging system with a scanned broadband supercontinuum light source. We observed the specific attachment of the functionalized gold plasmonic nanoparticles (AuNPs) targeting CD44(+) human breast cancer cells by conventional and by proposed hyperspectral darkfield microscopy. This wide-field and low phototoxic hyperspectral imaging system has been successful for performing spectral three-dimensional (3D) localization and spectroscopic identification of CD44-targeted PEGylated AuNPs in fixed cell preparations. Such spatial and spectral information is essential for the improvement of nanoplasmonic-based imaging, disease detection and treatment in complex biological environment. Presented system capability for 3D NP tracking will also enable investigation of specific sub-cellular activity with the use of NPs as spectral sensors.
authors.affiliation: École Polytechnique de Montréal, Laser Processing and Plasmonics Laboratory, Engineering Physics Department, Montréal, Québec, H3C 3A7, Canada.
authors.forename: Sergiy
authors.initials: S
authors.lastname: Patskovsky
authors.forename: Eric
authors.initials: E
authors.lastname: Bergeron
authors.forename: Michel
authors.initials: M
authors.lastname: Meunier
mesh_terms: D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006046:Gold / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D021621:Imaging, Three-Dimensional; D053768:Metal Nanoparticles / Q000737:chemistry*; D008853:Microscopy / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D018960:Hyaluronan Receptors; D011092:Polyethylene Glycols; D006046:Gold
keywords: Biomarker; cancer stem cell; darkfield imaging; functionalization; monoclonal antibody; nanosphere; poly(ethylene glycol); supercontinuum