title: Major constituents of Boswellia carteri resin exhibit cyclooxygenase enzyme inhibition and antiproliferative activity.
abstract: Aromatic gum from Boswellia carteri (olibanum oleogum) has long been used in Egyptian traditional medicine. Cyclooxygenase-1 (COX-1) enzyme inhibitory assay guided purification of the extracts of this resin resulted in five bioactive compounds, 3alpha-O-acetyl-8,24-dien-tirucallic acid (1), verticilla-4(20),7,11-triene (2), cembrene A (3), incensole acetate (4), and incensole (5). The pure isolates were investigated for their inhibitory effects on COX-1 and -2 enzymes and human tumor cell lines Hep-G2, MCF-7 and RAW 264.7. Compounds 1-5 inhibited COX-2 enzyme by 39.0, 32.7, 60.0, 46.3, and 49.8%, respectively. Furthermore, compound 2 showed an inhibitory concentration of 50% (IC50) at 9 microg/mL against Hep-G2 tumor cell line. This is the first report of COX-1 and -2 enzyme and tumor cell proliferation inhibitory effects of compounds 1 and 2.
authors.affiliation: Plant Biochemistry Department, National Research Centre, Dokki, Cairo, Egypt.
authors.forename: Sami I
authors.initials: SI
authors.lastname: Ali
authors.affiliation: Plant Biochemistry Department, National Research Centre, Dokki, Cairo, Egypt.
authors.forename: Chuan-Rui
authors.initials: CR
authors.lastname: Zhang
authors.affiliation: Plant Biochemistry Department, National Research Centre, Dokki, Cairo, Egypt.
authors.forename: Amal A
authors.initials: AA
authors.lastname: Mohamed
authors.affiliation: Plant Biochemistry Department, National Research Centre, Dokki, Cairo, Egypt.
authors.forename: Farouk K
authors.initials: FK
authors.lastname: El-Baz
authors.affiliation: Botany Department, Faculty of Science, Cairo University, Giza, Egypt.
authors.forename: Ahmad K
authors.initials: AK
authors.lastname: Hegazy
authors.affiliation: Botany Department, Faculty of Science, Cairo University, Giza, Egypt.
authors.forename: Maimona A
authors.initials: MA
authors.lastname: Kord
authors.affiliation: Bioactive Natural Products and Phytoceuticals Laboratory, Department of Horticulture, Michigan State University, East Lansing, 48824, Michigan, USA.
authors.forename: Muraleedharan G
authors.initials: MG
authors.lastname: Nair
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification*; D029743:Boswellia; D045744:Cell Line, Tumor; D052246:Cyclooxygenase 2 Inhibitors / Q000302:isolation & purification*; D004224:Diterpenes / Q000302:isolation & purification*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D053149:Plant Gums / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry
chemical_list: C585934:3alpha-O-acetyl-8,24-dien-tirucallic acid; D000972:Antineoplastic Agents, Phytogenic; D052246:Cyclooxygenase 2 Inhibitors; D004224:Diterpenes; D053149:Plant Gums; C530368:incensole acetate; C585935:verticilla-4(20),7,11-triene; C108204:cembrene A