title: Frequency dependent impedimetric cytotoxic evaluation of anticancer drug on breast cancer cell.
abstract: The present work reports the impedance characteristics of MCF-7 cell lines treated with anticancer drug ZD6474 to evaluate the cytotoxic effect on cellular electrical behaviour using miniature impedance sensors. Four types of impedance sensing devices with different electrode geometries are fabricated by microfabrication technology. The frequency response characteristics of drug treated cells are studied to evaluate cytotoxic effect of ZD6474 and also to assess the frequency dependent sensitivity variation with electrode area. A significant variation in magnitude of measured impedance data is obtained for drug treated samples above 10 µM dose indicating prominent effect of ZD6474 which results in suppression of cell proliferation and induction of apoptosis process. The results obtained by impedimetric method are correlated well with conventional in vitro assays such as flow cytometry, cell viability assays and microscopic imaging. Finally an empirical relation between cell impedance, electrode area and drug dose is established from impedance data which exhibits a negative correlation between drug doses and impedance of cancer cells.
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India. Electronic address: rangadhar@iitkgp.ac.in.
authors.forename: Rangadhar
authors.initials: R
authors.lastname: Pradhan
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Shashi
authors.initials: S
authors.lastname: Rajput
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Mahitosh
authors.initials: M
authors.lastname: Mandal
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Analava
authors.initials: A
authors.lastname: Mitra
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Soumen
authors.initials: S
authors.lastname: Das
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage; D017209:Apoptosis / Q000187:drug effects; D001681:Biological Assay / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000295:instrumentation*; D001943:Breast Neoplasms / Q000175:diagnosis / Q000188:drug therapy* / Q000503:physiopathology*; D049109:Cell Proliferation / Q000187:drug effects; D058266:Dielectric Spectroscopy / Q000295:instrumentation*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D010880:Piperidines / Q000008:administration & dosage*; D011799:Quinazolines / Q000008:administration & dosage*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents; D010880:Piperidines; D011799:Quinazolines; C452423:vandetanib
keywords: Bioimpedance; Cytotoxicity; MCF-7; Microfabrication; ZD6474