title: Comparative genomic and proteomic anatomy of Mycobacterium ubiquitous Esx family proteins: implications in pathogenicity and virulence.
abstract: Secreted proteins are among the most important molecules involved in host-pathogen interaction of Mycobacterium tuberculosis, the etiological agent of human tuberculosis (TB). M. tuberculosis encodes five types of VII secretion systems (ESX-1 to ESX-5) responsible for the exportation of many proteins. This system mediated substrates including members of the Esx family implicated in tuberculosis pathogenesis and survival within host cells. However, the distribution and evolution of this family remain elusive. To explore the evolution and distribution of Esx family proteins, we analyzed all available Mycobacteria genomes. Interestingly, amino mutations among M. tuberculosis esx family proteins may relate to their functions. We further analyzed the differences between pathogenic Mycobacteria, the attenuated Mycobacteria and non-pathogenic Mycobacteria. The stability, the globular domains and the phosphorylation of serine/threonine residues of M. tuberculosis esx proteins with their homologies among other Mycoabcteria were analyzed. Our comparative genomic and proteomic analysis found that the change of stability, gain or loss of globular domains and phosphorylation of serine/threonine might be responsible for the difference between the pathogenesis and virulence of the esx proteins and its homolog widespread among Mycobacteria and related species, which may provide clues for novel anti-tuberculosis drug targets.
authors.affiliation: Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Enviroment and Bio-Resource of the Three Gorges Area, Key Laboratory of Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Beibei, 400715, Chongqing, China, dengwanyan2009@163.com.
authors.forename: Wanyan
authors.initials: W
authors.lastname: Deng
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Xiang
authors.forename: Jianping
authors.initials: J
authors.lastname: Xie
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D023281:Genomics / Q000379:methods; D008969:Molecular Sequence Data; D005810:Multigene Family; D009161:Mycobacterium / Q000235:genetics* / Q000472:pathogenicity*; D040901:Proteomics / Q000379:methods*; D016415:Sequence Alignment; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins