title: Perch liver reaction to Triaenophorus nodulosus plerocercoids with an emphasis on piscidins 3, 4 and proliferative cell nuclear antigen (PCNA) expression.
abstract: Histopathological lesions caused by plerocercoids of Triaenophorus nodulosus within the liver of perch, Perca fluviatilis, from Lake Trasimeno were studied. Livers harbored 1-3 parasite larvae and pathological alterations were more marked in those with 3 plerocercoids. In the liver, larvae were encysted, surrounded by a capsule of host tissue; two of 14 plerocercoids were necrotic. In infected livers, some hepatocytes showed degenerative changes, i.e. swelling and hydropic degeneration, notably those in close proximity to larvae. By comparison, hepatocytes in uninfected livers or in regions away from the point of infection appeared normal. The occurrence of macrophage aggregates (MAs) distributed among the mast cells (MCs) was observed around the encysted larvae. The cellular elements involved in the immune response within liver were assessed by immunohistochemical techniques and by the use of antibodies against the antimicrobial peptides piscidins 3 and 4, which revealed a sub-population of positive MCs. In infected livers, numerous MCs that were immunopositive to P4 and a few that were positive to P3 were found around T. nodulosus larvae. Histological sections of both uninfected and infected liver were immunostained with proliferative cell nuclear antigen (PCNA) antibody. Within the capsule and in close proximity to the parasite larvae, various cell types (i.e., MCs, fibroblasts and epithelioid cells) and a significantly higher number of PCNA-positive hepatocytes that were immunoreactive to PCNA were found compared to uninfected livers (ANOVA, P<0.05). No parasites of any type were found in gill, spleen, kidney or gonad of P. fluviatilis and the intestine of 3 perch were infected with few specimens of Acanthocephalus lucii.
authors.affiliation: Department of Life Sciences and Biotechnology, University of Ferrara, St. Borsari 46, 44121 Ferrara, Italy. Electronic address: dzb@unife.it.
authors.forename: B S
authors.initials: BS
authors.lastname: Dezfuli
authors.affiliation: Department of Life Sciences and Biotechnology, University of Ferrara, St. Borsari 46, 44121 Ferrara, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Giari
authors.affiliation: Department of Cellular and Environmental Biology, University of Perugia, St. Elce di Sotto 5, 06123 Perugia, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Lorenzoni
authors.affiliation: Faculty of Biosciences, Agro-Alimentary and Environmental Technologies, University of Teramo, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Manera
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 St. Hillsborough, Raleigh, NC 27606, USA.
authors.forename: E J
authors.initials: EJ
authors.lastname: Noga
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics*; D002589:Cestoda / Q000276:immunology; D002590:Cestode Infections / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D005393:Fish Diseases / Q000276:immunology* / Q000473:pathology; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D007150:Immunohistochemistry; D008099:Liver / Q000276:immunology / Q000469:parasitology; D010471:Perches / Q000235:genetics / Q000276:immunology / Q000469:parasitology; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics*
chemical_list: D023181:Antimicrobial Cationic Peptides; D018809:Proliferating Cell Nuclear Antigen
keywords: Antimicrobial peptides; Cell proliferation; Cestode larvae; Fish; Immunohistochemistry; Mast cells