title: Bone regeneration with systemic administration of lactoferrin in non-critical-sized rat calvarial bone defects.
abstract: Using in vivo microfocus computed tomography (micro-CT) and tissue sections, we evaluated bone regeneration in non-critical-sized rat calvarial flat bone defects during systemic administration of lactoferrin (LF). Ten 11-week-old male Fischer rats were used. Non-critical-sized calvarial bone defects (diameter, 2.7 mm) were trephined into the dorsal parietal bone on both sides of the midsagittal suture, and a collagen sponge soaked in saline was placed on each side. LF was injected intraperitoneally every day, starting on the day of the operation (day 0; groups: control, 10 mg/kg LF, 100 mg/kg LF). Micro-CT imaging was performed repeatedly from 1 to 4 weeks after surgery. The defect sites were then removed, along with surrounding bone and soft tissues, and stained with hematoxylin and eosin. During weeks 1-4, micro-CT showed a significant difference in reossification ratio between the controls and the 100 mg/kg LF group. Histological analysis revealed that there were more osteoblast-like cells around the bony rim in the 100 mg/kg group than in the control group. In summary, micro-CT and histological analyses showed that systemic administration of LF accelerated bone regeneration in non-critical-sized rat calvarial bone defects.
authors.affiliation: Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yoshimaki
authors.forename: Shuichi
authors.initials: S
authors.lastname: Sato
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Tsunori
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Shino
authors.forename: Shinya
authors.initials: S
authors.lastname: Iguchi
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Arai
authors.forename: Koichi
authors.initials: K
authors.lastname: Ito
authors.forename: Bunnai
authors.initials: B
authors.lastname: Ogiso
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001861:Bone Regeneration; D002118:Calcium / Q000097:blood; D007781:Lactoferrin / Q000008:administration & dosage*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D012886:Skull / Q000473:pathology*; D055114:X-Ray Microtomography
chemical_list: D000469:Alkaline Phosphatase; D007781:Lactoferrin; D002118:Calcium