title: A new nano-optical sensor thin film cadmium sulfide doped in sol-gel matrix for assessment of α-amylase activity in human saliva.
abstract: A novel, simple, sensitive and precise spectrofluorimetric method is developed for measuring the activity of the α-amylase enzyme in human saliva. The remarkable quenching of the luminescence intensity at 634 nm of nano CdS doped in a sol-gel matrix by various concentrations of maltose (produced from the reaction of the enzyme with the starch substrate) was successfully used as an optical sensor for the assessment of α-amylase activity. The calibration plot was achieved over the concentration range 4.8 × 10(-10) to 1.2 × 10(-5) mol L(-1) maltose with a correlation coefficient of 0.999 and a detection limit of 5.7 × 10(-11) mol L(-1). The method was used satisfactorily for assessment of the α-amylase activity in a number of human saliva samples collected from various healthy volunteers.
authors.affiliation: Nanophotochemistry and Solarchemistry Lab, Department of Chemistry, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt. Mohamed_sam@yahoo.com.
authors.forename: M S
authors.initials: MS
authors.lastname: Attia
authors.forename: H
authors.initials: H
authors.lastname: Zoulghena
authors.forename: M S A
authors.initials: MS
authors.lastname: Abdel-Mottaleb
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry; D005782:Gels; D006801:Humans; D057230:Limit of Detection; D008320:Maltose / Q000737:chemistry / Q000378:metabolism; D036103:Nanotechnology / Q000379:methods; D061848:Optical Imaging / Q000379:methods; D012463:Saliva / Q000201:enzymology*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013440:Sulfides / Q000737:chemistry; D000516:alpha-Amylases / Q000032:analysis*
chemical_list: D019187:Cadmium Compounds; D005782:Gels; D013440:Sulfides; C034939:cadmium sulfide; D008320:Maltose; D000516:alpha-Amylases