title: [Prosapogenin A inhibits cell growth of MCF7 via downregulating STAT3 and glycometabolism-related gene].
abstract: This study is to investigate the inhibitory effect and mechanism of prosapogenin A (PSA) on MCF7. MTT assay was performed to determine the inhibitory effect of PSA on MCF7 cells. PI/Hoechst 33342 double staining was used to detect cell apoptosis. RT-PCR was used to test the mRNA levels of STAT3, GLUT1, HK and PFKL. Western blotting was performed to determine the expression of STAT3 and pSTAT3 protein in MCF7 cells. The results showed that PSA could dose-dependently inhibit cell growth of MCF7 followed by IC50 of 9.65 micrmol x L(-1) and promote cell apoptosis of MCF7. Reduced mRNA levels of STAT3, HK and PFKL were observed in MCF7 cells treated with 5 micromol x L(-1) of PSA. PSA also decreased the level of pSTAT3 protein. STAT3 siRNA caused decrease of mRNA of GLUT1, HK and PFKL which indicated STAT3 could regulate the expressions of GLUT1, HK and PFKL. The results suggested that PSA could inhibit cell growth and promote cell apoptosis of MCF7 via inhibition of STAT3 and glycometabolism-related gene.
authors.affiliation: Institute of Chinese Materia Medica, College of Pharmacy, Henan University, Kaifeng 475004, China. wtx1975@126.com
authors.forename: Tian-xiao
authors.initials: TX
authors.lastname: Wang
authors.affiliation: Institute of Chinese Materia Medica, College of Pharmacy, Henan University, Kaifeng 475004, China.
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Shi
authors.affiliation: Institute of Chinese Materia Medica, College of Pharmacy, Henan University, Kaifeng 475004, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Cong
authors.affiliation: Institute of Chinese Materia Medica, College of Pharmacy, Henan University, Kaifeng 475004, China.
authors.forename: Zhong-qing
authors.initials: ZQ
authors.lastname: Zhang
authors.affiliation: Institute of Chinese Materia Medica, College of Pharmacy, Henan University, Kaifeng 475004, China.
authors.forename: Ying-hua
authors.initials: YH
authors.lastname: Liu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D051272:Glucose Transporter Type 1 / Q000235:genetics / Q000378:metabolism; D006593:Hexokinase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D061986:MCF-7 Cells; D025221:Phosphofructokinases / Q000235:genetics / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D014703:Veratrum / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D051272:Glucose Transporter Type 1; D012333:RNA, Messenger; C495677:SLC2A1 protein, human; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D012503:Saponins; C026473:prosapogenin; D025221:Phosphofructokinases; D006593:Hexokinase