title: SIRT1 expression in the cochlea and auditory cortex of a mouse model of age-related hearing loss.
abstract: SIRT1 is a highly conserved NAD(+)-dependent protein deacetylase known to have protective effects against a variety of age-related diseases. However, there is a lack of information concerning SIRT1 expression in the cochlea and auditory cortex of C57BL/6 mice, a mouse model of age-related hearing loss. Using RT-PCR and immunohistochemistry, we show that SIRT1 is abundantly expressed in the inner hair cells, strial marginal cells, strial intermediate cells, type I and type IV fibrocytes of the spiral ligament and spiral ganglion neurons. In addition, moderate SIRT1 is also detected in the outer hair cells and neurons of the auditory cortex. Associated with elevated hearing thresholds and hair cells loss during aging, there is also a significant reduction of SIRT1 expression in the cochlea and auditory cortex. The expression pattern of SIRT1 in the peripheral and central auditory system suggests that SIRT1 may play an important role in auditory function and therefore may serve as a protective molecule against age-related hearing loss.
authors.affiliation: Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, China; Institute of Hearing and Speech-Language Science, Sun Yat-sen University, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Xiong
authors.affiliation: Department of Hematology, Nanfang Hospital, Southern Medical University, China.
authors.forename: Min
authors.initials: M
authors.lastname: Dai
authors.affiliation: Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, China; Institute of Hearing and Speech-Language Science, Sun Yat-sen University, China.
authors.forename: Yongkang
authors.initials: Y
authors.lastname: Ou
authors.affiliation: Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, China; Institute of Hearing and Speech-Language Science, Sun Yat-sen University, China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Pang
authors.affiliation: Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, China; Institute of Hearing and Speech-Language Science, Sun Yat-sen University, China.
authors.forename: Haidi
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, China; Institute of Hearing and Speech-Language Science, Sun Yat-sen University, China.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, China; Institute of Hearing and Speech-Language Science, Sun Yat-sen University, China.
authors.forename: Suijun
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, China; Institute of Hearing and Speech-Language Science, Sun Yat-sen University, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, China; Institute of Hearing and Speech-Language Science, Sun Yat-sen University, China.
authors.forename: Yaodong
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, China; Institute of Hearing and Speech-Language Science, Sun Yat-sen University, China.
authors.forename: Yuexin
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, China; Institute of Hearing and Speech-Language Science, Sun Yat-sen University, China.
authors.forename: Maojin
authors.initials: M
authors.lastname: Liang
authors.affiliation: Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, China; Institute of Hearing and Speech-Language Science, Sun Yat-sen University, China.
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, China; Institute of Hearing and Speech-Language Science, Sun Yat-sen University, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Lai
authors.affiliation: Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, China; Institute of Hearing and Speech-Language Science, Sun Yat-sen University, China. Electronic address: yiqingzheng@hotmail.com.
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D001303:Auditory Cortex / Q000378:metabolism*; D001309:Auditory Threshold / Q000502:physiology; D003051:Cochlea / Q000378:metabolism*; D004195:Disease Models, Animal; D016057:Evoked Potentials, Auditory, Brain Stem / Q000502:physiology; D006199:Hair Cells, Auditory, Inner / Q000378:metabolism; D018072:Hair Cells, Auditory, Outer / Q000378:metabolism; D008810:Mice, Inbred C57BL; D011304:Presbycusis / Q000209:etiology* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D056564:Sirtuin 1 / Q000378:metabolism* / Q000502:physiology; D013136:Spiral Ganglion / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; C447939:SIRT1 protein, human; D056564:Sirtuin 1
keywords: Auditory cortex; C57BL/6; Cochlea; Presbycusis; SIRT1