title: CUDC-101, a histone deacetylase inhibitor, improves the in vitro and in vivo developmental competence of somatic cell nuclear transfer pig embryos.
abstract: The aim of the present study was to examine the effects of CUDC-101, a novel histone deacetylase inhibitor, on the in vitro development and expression of the epigenetic marker histone H3 at lysine 9 (AcH3K9) in pig SCNT embryos. We found that treatment with 1 μmol/L CUDC-101 for 24 hours significantly improved the development of pig SCNT embryos. Compared with the control group, the blastocyst rate was higher (18.5% vs. 10.3%; P < 0.05). To assess in vivo developmental potency, CUDC-101-treated SCNT embryos were transferred into two surrogate mothers, resulting in one pregnancy with six fetuses. We then investigated the acetylation level of histone H3K9 in SCNT embryos treated with CUDC-101 and compared them only against untreated embryos. The acetylation level of control SCNT embryos was lower than that of CUDC-101-treated embryos at pseudo-pronuclear stages, and immunofluorescent signal for H3K9ac in CUDC-101-treated embryos in a pattern similar to that of control group. In conclusion, we demonstrated that CUDC-101 can significantly improve in vitro and in vivo developmental competence and enhance the nuclear reprogramming of pig SCNT embryos.
authors.affiliation: Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China.
authors.forename: Jun-Xue
authors.initials: JX
authors.lastname: Jin
authors.affiliation: Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China.
authors.forename: Suo
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Hong
authors.affiliation: Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China.
authors.forename: Long
authors.initials: L
authors.lastname: Jin
authors.affiliation: Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China.
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Zhu
authors.affiliation: Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China.
authors.forename: Qing-Shan
authors.initials: QS
authors.lastname: Gao
authors.affiliation: Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China.
authors.forename: Chang-Guo
authors.initials: CG
authors.lastname: Yan
authors.affiliation: Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China. Electronic address: kangjindan@hotmail.com.
authors.forename: Jin-Dan
authors.initials: JD
authors.lastname: Kang
authors.affiliation: Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China. Electronic address: yinxj33@msn.com.
authors.forename: Xi-Jun
authors.initials: XJ
authors.lastname: Yin
mesh_terms: D000818:Animals; D065150:Cellular Reprogramming / Q000187:drug effects / Q000502:physiology; D004624:Embryo Transfer / Q000662:veterinary; D004622:Embryo, Mammalian / Q000502:physiology*; D047108:Embryonic Development / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D006657:Histones / Q000502:physiology*; D006877:Hydroxamic Acids / Q000494:pharmacology*; D008856:Microscopy, Fluorescence / Q000662:veterinary; D011247:Pregnancy; D011799:Quinazolines / Q000494:pharmacology*; D013552:Swine / Q000502:physiology*
chemical_list: C549566:7-(4-(3-ethynylphenylamino)-7-methoxyquinazolin-6-yloxy)-N-hydroxyheptanamide; D004791:Enzyme Inhibitors; D006657:Histones; D006877:Hydroxamic Acids; D011799:Quinazolines
keywords: Blastocyst; CUDC-101; Histone deacetylase inhibitor (HDACi); In vitro development; Somatic cell nuclear transfer (SCNT)