title: Proteinaceous protease inhibitor from Lawsonia inermis: purification, characterization and antibacterial activity.
abstract: A thermo-stable, proteinaceous protease inhibitor (LPI) from Lawsonia inermis is reported. The LPI was purified from Lawsonia inermis seeds by subsequent ammonium sulfate precipitation, ion exchange chromatography (DEAE-Cellulose) and gel permeation chromatography (Sephadex-50). The purified protease inhibitor is effective against a wide range of proteases viz. papain, trypsin, pepsin and metallo-protease. The apparent molecular weight of the protease inhibitor is 19 kDa, determined by SDS-PAGE electrophoresis. The protease inhibitor was found to be stable at 70 degrees C for 30 min. It was also examined for antibacterial activity against Pseudomonas aeruginosa MTCC 7926 and Staphylococcus aureus NCIM 2079; the IC50 values of the purified LPI were 11.4 microg/mL and 16.6 microg/mL respectively.
authors.affiliation: Department of Microbiology and Biotechnology, R. C. Patel Arts, Commerce and Science College, Shirpur 425405, India.
authors.forename: Arvind
authors.initials: A
authors.lastname: Dabhade
authors.affiliation: Department of Microbiology and Biotechnology, R. C. Patel Arts, Commerce and Science College, Shirpur 425405, India.
authors.forename: Priti
authors.initials: P
authors.lastname: Patel
authors.affiliation: Department of Microbiology and Biotechnology, R. C. Patel Arts, Commerce and Science College, Shirpur 425405, India.
authors.forename: Ulhas
authors.initials: U
authors.lastname: Pati
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D031564:Lawsonia Plant / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D011480:Protease Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D010940:Plant Proteins; D011480:Protease Inhibitors