title: [Curcumin inhibits HeLa cell invasion and migration by decreasing inducible nitric oxide synthase].
abstract: OBJECTIVE
  To investigate the inhibitory effects of curcumin against HeLa cell invasion and migration and explore the underlying mechanisms.
  
  
  METHODS
  HeLa cells were exposed to curcumin treatment at the concentrations of 0, 10, 25, 50, 100, 150 and 200 µmol/L for 24 h. MTT and TUNEL assays were used to assess the cell proliferation inhibition and apoptosis, respectively. Transwell assay was used to evaluate the invasiveness and migration of the treated cells, and RT-PCR and Western blotting were employed to detect the changes in the expression of inducible nitric oxide synthase (iNOS), and MMP-9 and E-cad, the 2 markers of cell invasion and migration, were detected by Western blotting. The capacity of NO production in HeLa cells was measured by Griess method.
  
  
  RESULTS
  Curcumin inhibited the proliferation of HeLa cells by inducing cell apoptosis in a concentration-dependent manner. Curcumin inhibited the invasion and migration of HeLa cells by increasing E-cad expression and decreasing MMP-9 expression, and also decreased the expression level of iNOS and NO production in the cells.
  
  
  CONCLUSIONS
  Curcumin inhibits the invasion and migration of HeLa cells by decreasing the expression of iNOS.
authors.affiliation: Department of Gynecology and Obstetrics, Second Affiliated Hospital, Xi'an Jiaotong University College of Medicine, Xi'an 710004, China. E-mail: muli127@126.com.
authors.forename: Mu
authors.initials: M
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Haili
authors.initials: H
authors.lastname: Liu
authors.forename: Baoshan
authors.initials: B
authors.lastname: Su
authors.forename: Bianli
authors.initials: B
authors.lastname: Liu
authors.forename: Wenjing
authors.initials: W
authors.lastname: Lin
authors.forename: Zhaorong
authors.initials: Z
authors.lastname: Li
authors.forename: Lihua
authors.initials: L
authors.lastname: Chang
mesh_terms: D017209:Apoptosis; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation; D003474:Curcumin / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*
chemical_list: C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9; D003474:Curcumin