title: Chimonanthus nitens var. salicifolius Aqueous Extract Protects against 5-Fluorouracil Induced Gastrointestinal Mucositis in a Mouse Model.
abstract: Gastrointestinal mucositis is a major side effect of chemotherapy, leading to life quality reduction in patients and interrupting the therapy of cancer. Chimonanthus nitens var. salicifolius (CS) is a traditional Chinese herb for enteral disease. Considering the protective effect of CS on intestine, we hypothesize that the aqueous extract of CS could be benefcial to gastrointestinal mucositis. To verify this, a mouse mucositis model was induced by 5-Fluorouracil (5-Fu). Male Balb/C mice were treated with CS aqueous extract (5, 10, and 20 g/kg) or loperamide (0.2 mg/kg) intragastrically for 11 days, and the severity of mucositis was evaluated. Furthermore, the chemical compounds of CS aqueous extract were also analysed by high-performance liquid chromatography (HPLC). Our results demonstrated that CS aqueous extract improved mice body weight, diarrhoea, and faecal blood, maintained the liver function and intestinal length, alleviated villus shortening, and suppressed the apoptosis and inflammation in small intestine. We concluded that CS could protect mice against 5-Fu induced mucositis by inhibiting apoptosis and inflammation, and this protective effect might be associated with the 3 flavonoids (rutin, quercetin, and kaempferol) identified in CS aqueous extract.
authors.affiliation: School of Life Science and Technology, Tongji University, Shanghai 200092, China ; The Sino-Australia Joint Laboratory, Lishui Institute of Traditional Chinese Medicine, Tongji University, Lishui 323000, China.
authors.forename: Zhenze
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Shanghai Research Centre for Model Organisms, Shanghai 201203, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xi
authors.affiliation: HanseMerkur Centre for Traditional Chinese Medicine at the University Medical Centre Hamburg-Eppendorf, Haus Ost 55, UKE Campus, Martinistraße 52, 20246 Hamburg, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Schröder
authors.affiliation: Shanghai Research Centre for Model Organisms, Shanghai 201203, China.
authors.forename: Weigang
authors.initials: W
authors.lastname: Wang
authors.affiliation: Shanghai Standard Biotech Co., Ltd., Shanghai 201203, China.
authors.forename: Tianpei
authors.initials: T
authors.lastname: Xie
authors.affiliation: Shanghai Research Centre for Model Organisms, Shanghai 201203, China.
authors.forename: Zhugang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: The Sino-Australia Joint Laboratory, Lishui Institute of Traditional Chinese Medicine, Tongji University, Lishui 323000, China ; Discipline of Pathology, Bosch Institute and School of Medical Sciences, University of Sydney, NSW 2006, Australia.
authors.forename: Shisan
authors.initials: S
authors.lastname: Bao
authors.affiliation: School of Life Science and Technology, Tongji University, Shanghai 200092, China ; The Sino-Australia Joint Laboratory, Lishui Institute of Traditional Chinese Medicine, Tongji University, Lishui 323000, China ; Shanghai Research Centre for Model Organisms, Shanghai 201203, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Fei