title: Cell migration and division in amoeboid-like fission yeast.
abstract: Yeast cells are non-motile and are encased in a cell wall that supports high internal turgor pressure. The cell wall is also essential for cellular morphogenesis and cell division. Here, we report unexpected morphogenetic changes in a Schizosaccharomyces pombe mutant defective in cell wall biogenesis. These cells form dynamic cytoplasmic protrusions caused by internal turgor pressure and also exhibit amoeboid-like cell migration resulting from repeated protrusive cycles. The cytokinetic ring responsible for cell division in wild-type yeast often fails in these cells; however, they were still able to divide using a ring-independent alternative mechanism relying on extrusion of the cell body through a hole in the cell wall. This mechanism of cell division may resemble an ancestral mode of division in the absence of cytokinetic machinery. Our findings highlight how a single gene change can lead to the emergence of different modes of cell growth, migration and division.
authors.affiliation: Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide-Consejo Superior de Investigaciones Científicas, 41013 Sevilla, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Flor-Parra
authors.forename: Manuel
authors.initials: M
authors.lastname: Bernal
authors.forename: Jacob
authors.initials: J
authors.lastname: Zhurinsky
authors.forename: Rafael R
authors.initials: RR
authors.lastname: Daga
keywords: Cell migration; Cytokinesis; Fission yeast; Morphogenesis; Turgor pressure