title: Microarray technologies for intracellular kinome analysis.
abstract: Microarray-based kinomics, which measure the enzymatic activity or the presence of intracellular protein kinases, are now regarded as alternative tools to conventional mass spectrometry-based kinomics for examining intracellular kinomics. Here, we reviewed the principal advantages, recent progress, and remaining problems of representative microarray- based kinomics, including substrate peptide and protein microarrays, anti-protein kinase antibody microarrays, and reverse protein microarrays. Microarray-based kinomics are not as good at quantitative evaluation of kinomics as the conventional mass spectrometry-based kinomics. However, their simplicity and high throughput make the microarraybased kinomics unique tools, being especially suited for a practical analysis; monitoring drug effects on cellular kinomics as a tool for drug development, and for the diagnosis and prognosis of diseases based on kinomics.
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.affiliation: Department of Applied Chemistry, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819- 0395, Japan. ykatatcm@mail.cstm.kyushu-u.ac.jp.
authors.forename: Y
authors.initials: Y
authors.lastname: Katayama
mesh_terms: D006801:Humans; D042541:Intracellular Space / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D046228:Microarray Analysis / Q000379:methods*; D011494:Protein Kinases / Q000378:metabolism*; D040901:Proteomics
chemical_list: D011494:Protein Kinases