title: Transfer of serum and cells from Yersinia ruckeri vaccinated doubled-haploid hot creek rainbow trout into outcross F1 progeny elucidates mechanisms of vaccine-induced protection.
abstract: Yersinia ruckeri is a well-established bacterial pathogen for many salmonid species, against which a formalin-killed bacterin vaccine has been effective in reducing disease outbreaks. Previous studies have reported conflicting results about the protective value of the systemic humoral response to Y. ruckeri vaccination. Here we directly demonstrate that plasma contains the long-term protective component elicited by both immersion and intraperitoneal injection vaccination of rainbow trout. A total of 0.5 μL of plasma from vaccinated fish provided almost complete protection against experimental challenge. Conversely, the cells obtained from peripheral blood conferred little or no protection in naïve recipients. The protective component of immune sera was IgM based on size exclusion chromatography and recognition by monoclonal antibody Warr 1-14. Immune plasma generated against a Y. ruckeri biotype 1 strain protected equally against challenges with Y. ruckeri biotype 1 and 2 strains. These results illustrate the importance of the humoral IgM response against Y. ruckeri and the use of doubled haploid rainbow trout (Oncorhynchus mykiss) and transfer of plasma/serum and cells into F1 outcross progeny as a model system for dissection of the mechanism(s) of vaccine-induced protection.
authors.affiliation: USDA-ARS, National Center for Cool and Cold Water Aquaculture, 11861 Leetown Rd, Kearneysville, WV 25430, USA. Electronic address: jason.evenhuis@ars.usda.gov.
authors.forename: Jason P
authors.initials: JP
authors.lastname: Evenhuis
authors.affiliation: USDA-ARS, National Center for Cool and Cold Water Aquaculture, 11861 Leetown Rd, Kearneysville, WV 25430, USA.
authors.forename: Gregory D
authors.initials: GD
authors.lastname: Wiens
authors.affiliation: School of Biological Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA 99164-4236, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Wheeler
authors.affiliation: USDA-ARS, National Center for Cool and Cold Water Aquaculture, 11861 Leetown Rd, Kearneysville, WV 25430, USA.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Welch
authors.affiliation: Clear Springs Foods Inc., Research Division, Buhl, ID 83316, USA.
authors.forename: Scott E
authors.initials: SE
authors.lastname: LaPatra
authors.affiliation: School of Biological Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA 99164-4236, USA.
authors.forename: Gary H
authors.initials: GH
authors.lastname: Thorgaard
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D001428:Bacterial Vaccines; D002478:Cells, Cultured; D006238:Haploidy; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007075:Immunoglobulin M / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000637:transplantation; D017686:Oncorhynchus mykiss / Q000235:genetics / Q000276:immunology*; D010949:Plasma / Q000378:metabolism*; D014611:Vaccination; D015009:Yersinia Infections / Q000276:immunology*; D044124:Yersinia ruckeri / Q000276:immunology*
chemical_list: D001428:Bacterial Vaccines; D007075:Immunoglobulin M
keywords: Adoptive transfer; IgM; Passive immunization; Rainbow trout; Yersinia ruckeri