title: Cytotoxic constituents of Pachyrhizus tuberosus from Peruvian amazon.
abstract: Investigations into the chemical constituents of the seeds of the neglected tuber crop Pachyrhizus tuberosus (Leguminosae) resulted in the isolation of seven components: five rotenoids [12a-hydroxyerosone (1), 12a-hydroxydolineone (2), erosone (3), 12a-hydroxyrotenone (4) and rotenone (6)], a phenylfuranocoumarin [pachyrrhizine (5)] and an isoflavanone [neotenone (7)]. The compounds were isolated using several chromatography techniques and characterized and verified by NMR and HPLC/MS. The MTT assay was used to examine the selective cytotoxic effects of the methanolic P. tuberosus extract and isolated compounds in two human cancer cell lines [breast (MCF-7) and colorectal (HCT-116)] and in non-transformed human fibroblasts (MRC-5); IC50 values were calculated. The methanolic P. tuberosus extract displayed respectable cytotoxic effects against HCT-116 and MCF-7 cells with IC50 values of 7.3 and 6.3 microg/mL, respectively. Of the compounds, 6 exacted greatest cytotoxicity and selectivity towards the cancer cell lines tested, yielding IC50 values of 0.3 microg/mL against both MCF-7 and HCT-116 cells, and a 6-fold reduced activity against MRC-5 fibroblasts. Compound 4 also demonstrated cytotoxicity against MCF-7 and HCT-116 (1.1 and 1.8 microg/mL, respectively), and reduced cytotoxicity towards MRC-5 cells (7.5 mirog/mL). The results revealed from the in vitro cytotoxic MTT assay are worthy of further antitumor investigation.
authors.affiliation: Department of Crop Sciences and Agroforestry, Faculty of Tropical Agri Sciences, Czech University of Life Sciences Prague, Kamycka 129, Prague 6, 165 21, Czech Republic.
authors.forename: Olga
authors.initials: O
authors.lastname: Leuner
authors.affiliation: Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, Prague 6, 165 21, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Havlik
authors.affiliation: lnstitute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo n. 2, 166 10 Prague 6, Czech Republic.
authors.forename: Milos
authors.initials: M
authors.lastname: Budesinsky
authors.affiliation: lnstitute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo n. 2, 166 10 Prague 6, Czech Republic.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Vrkoslav
authors.affiliation: Centre for Biomolecular Sciences, School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 2RD, UK.
authors.forename: Jessica
authors.initials: J
authors.lastname: Chu
authors.affiliation: Centre for Biomolecular Sciences, School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 2RD, UK.
authors.forename: Tracey D
authors.initials: TD
authors.lastname: Bradshaw
authors.affiliation: Department of Crop Sciences and Agroforestry, Faculty of Tropical Agri Sciences, Czech University of Life Sciences Prague, Kamycka 129, Prague 6, 165 21, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Hummelova
authors.affiliation: Department of Chemistry of Natural Compounds, Institute of Chemical Technology Prague, Technicka 5, 166 28 Prague 6, Czech Republic.
authors.forename: Petra
authors.initials: P
authors.lastname: Miksatkova
authors.affiliation: Department of Chemistry of Natural Compounds, Institute of Chemical Technology Prague, Technicka 5, 166 28 Prague 6, Czech Republic.
authors.forename: Oldrich
authors.initials: O
authors.lastname: Lapcik
authors.affiliation: lnstitute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo n. 2, 166 10 Prague 6, Czech Republic.
authors.forename: Irena
authors.initials: I
authors.lastname: Valterova
authors.affiliation: Department of Crop Sciences and Agroforestry, Faculty of Tropical Agri Sciences, Czech University of Life Sciences Prague, Kamycka 129, Prague 6, 165 21, Czech Republic.
authors.forename: Ladislav
authors.initials: L
authors.lastname: Kokoska
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification*; D004354:Drug Screening Assays, Antitumor; D045325:HCT116 Cells; D006801:Humans; D061986:MCF-7 Cells; D015394:Molecular Structure; D029905:Pachyrhizus / Q000737:chemistry*; D012402:Rotenone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C585945:erosone; D012402:Rotenone