title: Osteoblast cell behavior on the new beta-type Ti-25Ta-25Nb alloy.
abstract: Among metallic materials used as bone substitutes, β titanium alloys gain an increasing importance because of their low modulus, high corrosion resistance and good biocompatibility. In this work, an investigation of the in vitro cytocompatibility of a recently new developed β-type Ti-25Ta-25Nb alloy was carried out by evaluating the behavior of human osteoblasts. The metallic Ti-6Al-4V biomaterial, which is one of representative α+β type titanium alloys for biomedical applications, and Tissue Culture Polystyrene (TCPS), were also investigated as reference Ti-based material and control substrate, respectively. Both metallic surfaces were analyzed by X-ray diffraction, atomic force microscopy and X-ray photoelectron spectroscopy. The cellular response was quantified by assessments of viability, cell attachment and spreading, cell morphology, production and extracellular organization of fibronectin and cell proliferation. Polished surfaces from both materials having an equiaxed grain microstructure and nanometre scale surface roughness elicited an essentially identical osteoblast response in terms of all analyzed cellular parameters. Thus, on both surfaces the cells displayed high survival rates, good cell adhesion and spreading, a dense and randomly dispersed fibronectin matrix and increasing cell proliferation rates over the incubation time. Furhermore, the enhanced biological performance of Ti-25Ta-25Nb was highly supported by the results obtained in comparison with TCPS. These findings, together with previously shown superelastic behavior, low Young's modulus and high corrosion resistance, recommend Ti-25Ta-25Nb as good candidate for applications in bone implantology.
authors.affiliation: University of Bucharest, Department of Biochemistry and Molecular Biology, 91-95 Spl. Independentei, 050095, Bucharest, Romania.
authors.forename: Anisoara
authors.initials: A
authors.lastname: Cimpean
authors.affiliation: University of Bucharest, Department of Biochemistry and Molecular Biology, 91-95 Spl. Independentei, 050095, Bucharest, Romania.
authors.forename: Valentina
authors.initials: V
authors.lastname: Mitran
authors.affiliation: University of Bucharest, Department of Biochemistry and Molecular Biology, 91-95 Spl. Independentei, 050095, Bucharest, Romania.
authors.forename: Cristina M
authors.initials: CM
authors.lastname: Ciofrangeanu
authors.affiliation: University of Bucharest, Department of Biochemistry and Molecular Biology, 91-95 Spl. Independentei, 050095, Bucharest, Romania.
authors.forename: Bianca
authors.initials: B
authors.lastname: Galateanu
authors.affiliation: INSA Rennes, UMR CNRS 6226 SCR/Chimie-Métallurgie, 20 avenue des Buttes de Coësmes, F-35043 Rennes Cedex, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Bertrand
authors.affiliation: INSA Rennes, UMR CNRS 6226 SCR/Chimie-Métallurgie, 20 avenue des Buttes de Coësmes, F-35043 Rennes Cedex, France.
authors.forename: Doina-Margareta
authors.initials: DM
authors.lastname: Gordin
authors.affiliation: University of Bucharest, Department of Biochemistry and Molecular Biology, 91-95 Spl. Independentei, 050095, Bucharest, Romania.
authors.forename: Dana
authors.initials: D
authors.lastname: Iordachescu
authors.affiliation: INSA Rennes, UMR CNRS 6226 SCR/Chimie-Métallurgie, 20 avenue des Buttes de Coësmes, F-35043 Rennes Cedex, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Gloriant
mesh_terms: D000497:Alloys / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D001842:Bone and Bones / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D008422:Materials Testing / Q000379:methods; D010006:Osteoblasts / Q000502:physiology*; D056951:Photoelectron Spectroscopy / Q000379:methods; D015996:Survival Rate; D014025:Titanium / Q000737:chemistry*; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; C023988:beta titanium; D014025:Titanium