title: Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy.
abstract: In this study, a rapid and simple method which combines drop coating deposition and Raman spectroscopy (DCDR) was developed to characterize the dry embryo culture media (ECM) droplet. We demonstrated that Raman spectra obtained from the droplet edge presented useful and characteristic signatures for protein and amino acids assessment. Using a different analytical method, scanning electron microscopy coupled with energy dispersive X-ray analysis, we further confirmed that Na, K, and Cl were mainly detected in the central area of the dry ECM droplet while sulphur, an indicative of the presence of macromolecules such as proteins, was mainly found at the periphery of the droplet. In addition, to reduce sample preparation time, different temperatures for drying the droplets were tested. The results showed that drying temperature at 50°C can effectively reduce the sample preparation time to 6 min (as compared to 50 min for drying at room temperature, ∼25°C) without inducing thermal damage to the proteins. This work demonstrated that DCDR has potential for rapid and reliable metabolomic profiling of ECM in clinical applications.
authors.affiliation: Fujian Normal University, Key Laboratory of Opto-Electronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fuzhou 350007, China.
authors.forename: Zufang
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Fujian Maternal and Child Health Hospital, Fuzhou 350001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Fujian Normal University, Key Laboratory of Opto-Electronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fuzhou 350007, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Fujian Maternal and Child Health Hospital, Fuzhou 350001, China.
authors.forename: Shengrong
authors.initials: S
authors.lastname: Du
authors.affiliation: Fujian Normal University, Key Laboratory of Opto-Electronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fuzhou 350007, China.
authors.forename: Yongzeng
authors.initials: Y
authors.lastname: Li
authors.affiliation: Fujian Normal University, Key Laboratory of Opto-Electronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fuzhou 350007, China.
authors.forename: Juqiang
authors.initials: J
authors.lastname: Lin
authors.affiliation: Fujian Normal University, Key Laboratory of Opto-Electronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fuzhou 350007, China.
authors.forename: Shangyuan
authors.initials: S
authors.lastname: Feng
authors.affiliation: Fujian Normal University, Key Laboratory of Opto-Electronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fuzhou 350007, China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Lei
authors.affiliation: Fujian Normal University, Key Laboratory of Opto-Electronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fuzhou 350007, China.
authors.forename: Hongxin
authors.initials: H
authors.lastname: Lin
authors.affiliation: Fujian Normal University, Key Laboratory of Opto-Electronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fuzhou 350007, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
authors.affiliation: Fujian Normal University, Key Laboratory of Opto-Electronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fuzhou 350007, ChinacImaging Unit-Integrative Oncology Department, British Columbia Cancer Agency Research Centre, Vancouver, British Columbia V5Z 1L3, Canada.
authors.forename: Haishan
authors.initials: H
authors.lastname: Zeng
mesh_terms: D001755:Blastocyst / Q000378:metabolism*; D017077:Culture Media, Conditioned / Q000737:chemistry*; D006801:Humans; D013052:Spectrometry, X-Ray Emission; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D017077:Culture Media, Conditioned