title: Altered distribution of extracellular matrix proteins in the periodontal ligament of periostin-deficient mice.
abstract: Verifying whether periostin affects the distribution of type I collagen, fibronectin and tenascin C in the periodontal ligament (PDL) is important to contribute to a more thorough understanding of that protein's functions. In this study, we have histologically examined incisor PDL of mandibles in 20 week-old male wild-type and periostin-deficient (periostin-/-) mice, by means of type I collagen, fibronectin, tenascin C, proliferating cell nuclear antigen, matrix metallo-proteinase (MMP)-1 and F4/80-positive monocyte/macrophage immunostaining, transmission electron microscopy and quantitative analysis of cell proliferation. Wild-type PDL featured well-arranged layers of collagen bundles intertwined with PDL cells, whose longitudinal axis ran parallel to the collagen fibers. However, cells in the periostin-/- PDL were irregularly distributed among collagen fibrils, which were also haphazardly arranged. Type I collagen and fibronectin reactivity was seen throughout the wild-type PDL, while in the periostin-/- PDL, only focal, uneven staining for these proteins could be seen. Similarly, tenascin C staining was evenly distributed in the wild-type PDL, but hardly seen in the periostin-/- PDL. MMP-1 immunoreactivity was uniformly distributed in the wild-type PDL, but only dotted staining could be discerned in the periostin-/- PDL. F4/80-positive monocyte/macrophages were found midway between tooth- and bone-related regions in the wild-type PDL, a pattern that could not be observed in the periostin-/- PDL. In summary, periostin deficiency may not only cause PDL collagen fibril disorganization, but could also affect the distribution of other major extracellular matrix proteins such as fibronectin and tenascin C.
authors.affiliation: Departments of Developmental Biology of Hard Tissue and Orthodontics, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Chihiro
authors.initials: C
authors.lastname: Tabata
authors.affiliation: Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Hongo
authors.affiliation: Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Muneteru
authors.initials: M
authors.lastname: Sasaki
authors.affiliation: Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Tomoka
authors.initials: T
authors.lastname: Hasegawa
authors.affiliation: Dental School, Federal University of Sergipe at Lagarto, Brazil.
authors.forename: Paulo Henrique Luiz
authors.initials: PH
authors.lastname: de Freitas
authors.affiliation: Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Tamaki
authors.initials: T
authors.lastname: Yamada
authors.affiliation: Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Tomomaya
authors.initials: T
authors.lastname: Yamamoto
authors.affiliation: Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Suzuki
authors.affiliation: Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Tsuneyuki
authors.initials: T
authors.lastname: Yamamoto
authors.affiliation: Division of Biochemistry, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors.forename: Kimimitsu
authors.initials: K
authors.lastname: Oda
authors.affiliation: Shandong Provincial Key Laboratory of Oral Biomedicine, The School of Stomatology, Shandong University, Jinan, China.
authors.forename: Minqi
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Biological Information, Tokyo Institute of Technology, Yokohama, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Kudo
authors.affiliation: Department of Orthodontics, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Junichiro
authors.initials: J
authors.lastname: Iida
authors.affiliation: Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan. amizuka@den.hokudai.ac.jp.
authors.forename: Norio
authors.initials: N
authors.lastname: Amizuka
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation / Q000502:physiology; D024042:Collagen Type I / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D010513:Periodontal Ligament / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D019063:Tenascin / Q000378:metabolism
chemical_list: D000943:Antigens, Differentiation; D015815:Cell Adhesion Molecules; D024042:Collagen Type I; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; C082838:Postn protein, mouse; D018809:Proliferating Cell Nuclear Antigen; D019063:Tenascin; C036763:monocyte-macrophage differentiation antigen; D020781:Matrix Metalloproteinase 1