title: Stability of Bovine viral diarrhea virus 1 nucleic acid in fetal bovine samples stored under different conditions.
abstract: Infection of pregnant cattle with both species of Bovine viral diarrhea virus (BVDV) can result in reproductive disease that includes fetal reabsorption, mummification, abortion, stillbirths, congenital defects affecting structural, neural, reproductive, and immune systems, and the birth of calves persistently infected with BVDV. Accurate diagnosis of BVDV-associated reproductive disease is important to control BVDV at the production unit level and assessment of the cost of BVDV infections in support of BVDV control programs. The purpose of the current study was to examine the stability of viral nucleic acid in fetal tissues exposed to different conditions, as measured by detection by polymerase chain reaction. Five different types of fetal tissue, including brain, skin and muscle, ear, and 2 different pooled organ samples, were subjected to conditions that mimicked those that might exist for samples collected after abortions in production settings or possible storage conditions after collection and prior to testing. In addition, tissues were archived for 36 months at -20°C and then retested, to mimic conditions that might occur in the case of retrospective surveillance studies. Brain tissue showed the highest stability under the conditions tested. The impact of fecal contamination was increased following archiving in all tissue types suggesting that, for long-term storage, effort should be made to reduce environmental contaminants before archiving.
authors.affiliation: 1Julia F. Ridpath, NADC/ARS/USDA, 1920 Dayton Avenue, PO Box 70, Ames, IA 50010. julia.ridpath@ars.usda.gov.
authors.forename: Julia F
authors.initials: JF
authors.lastname: Ridpath
authors.forename: John D
authors.initials: JD
authors.lastname: Neill
authors.forename: Yu-Wei
authors.initials: YW
authors.lastname: Chiang
authors.forename: Jill
authors.initials: J
authors.lastname: Waldbillig
mesh_terms: D037881:Aborted Fetus; D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000517:prevention & control / Q000821:virology*; D002417:Cattle; D030241:Diarrhea Virus 1, Bovine Viral / Q000235:genetics / Q000302:isolation & purification*; D030242:Diarrhea Virus 2, Bovine Viral / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005333:Fetus / Q000821:virology*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000662:veterinary* / Q000821:virology; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013048:Specimen Handling / Q000592:standards / Q000662:veterinary*
chemical_list: D012367:RNA, Viral
keywords: Bovine viral diarrhea virus; detection; fetal; persistent infection; polymerase chain reaction