title: In vivo tomographic imaging with fluorescence and MRI using tumor-targeted dual-labeled nanoparticles.
abstract: Dual-modality imaging combines the complementary advantages of different modalities, and offers the prospect of improved preclinical research. The combination of fluorescence imaging and magnetic resonance imaging (MRI) provides cross-validated information and direct comparison between these modalities. Here, we report on the application of a novel tumor-targeted, dual-labeled nanoparticle (NP), utilizing iron oxide as the MRI contrast agent and near infrared (NIR) dye Cy5.5 as the fluorescent agent. Results of in vitro experiments verified the specificity of the NP to tumor cells. In vivo tumor targeting and uptake of the NPs in a mouse model were visualized by fluorescence and MR imaging collected at different time points. Quantitative analysis was carried out to evaluate the efficacy of MRI contrast enhancement. Furthermore, tomographic images were also acquired using both imaging modalities and cross-validated information of tumor location and size between these two modalities was revealed. The results demonstrate that the use of dual-labeled NPs can facilitate the dual-modal detection of tumors, information cross-validation, and direct comparison by combing fluorescence molecular tomography (FMT) and MRI.
authors.affiliation: Department of Biomedical Engineering, School of Medicine, Beijing, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Biomedical Engineering, School of Medicine, Beijing, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Biomedical Engineering, School of Medicine, Beijing, People's Republic of China ; Tsinghua-Peking Center for Life Sciences, Beijing, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Biomedical Engineering, School of Medicine, Beijing, People's Republic of China ; Center for Biomedical Imaging Research, Tsinghua University, Beijing, People's Republic of China.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Biomedical Engineering, School of Medicine, Beijing, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Bai
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology*; D002232:Carbocyanines; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry; D005456:Fluorescent Dyes; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*; D064847:Multimodal Imaging / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods; D014054:Tomography / Q000379:methods*
chemical_list: C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D003287:Contrast Media; D005290:Ferric Compounds; D005456:Fluorescent Dyes; C000499:ferric oxide
keywords: dual-modality; fluorescence molecular tomography (FMT); magnetic resonance imaging (MRI); nanoparticle