title: TRIM32-dependent transcription in adult neural progenitor cells regulates neuronal differentiation.
abstract: In the adult mammalian brain, neural stem cells in the subventricular zone continuously generate new neurons for the olfactory bulb. Cell fate commitment in these adult neural stem cells is regulated by cell fate-determining proteins. Here, we show that the cell fate-determinant TRIM32 is upregulated during differentiation of adult neural stem cells into olfactory bulb neurons. We further demonstrate that TRIM32 is necessary for the correct induction of neuronal differentiation in these cells. In the absence of TRIM32, neuroblasts differentiate slower and show gene expression profiles that are characteristic of immature cells. Interestingly, TRIM32 deficiency induces more neural progenitor cell proliferation and less cell death. Both effects accumulate in an overproduction of adult-generated olfactory bulb neurons of TRIM32 knockout mice. These results highlight the function of the cell fate-determinant TRIM32 for a balanced activity of the adult neurogenesis process.
authors.affiliation: 1] Westfälische Wilhelms-Universität Münster, ZMBE, Institute of Cell Biology, Stem Cell Biology and Regeneration Group, Von-Esmarch-Straße 56, Münster D-48149, Germany [2] Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Esch-Belval, Luxembourg.
authors.forename: A-L
authors.initials: AL
authors.lastname: Hillje
authors.affiliation: 1] Westfälische Wilhelms-Universität Münster, ZMBE, Institute of Cell Biology, Stem Cell Biology and Regeneration Group, Von-Esmarch-Straße 56, Münster D-48149, Germany [2] Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Esch-Belval, Luxembourg.
authors.forename: M A S
authors.initials: MA
authors.lastname: Pavlou
authors.affiliation: 1] Westfälische Wilhelms-Universität Münster, ZMBE, Institute of Cell Biology, Stem Cell Biology and Regeneration Group, Von-Esmarch-Straße 56, Münster D-48149, Germany [2] Department of Behavioural Biology, Westfälische Wilhelms-Universität Münster, Badestrasse 13, Muenster D-48149, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Beckmann
authors.affiliation: Westfälische Wilhelms-Universität Münster, ZMBE, Institute of Cell Biology, Stem Cell Biology and Regeneration Group, Von-Esmarch-Straße 56, Münster D-48149, Germany.
authors.forename: M M A
authors.initials: MM
authors.lastname: Worlitzer
authors.affiliation: 1] Westfälische Wilhelms-Universität Münster, ZMBE, Institute of Cell Biology, Stem Cell Biology and Regeneration Group, Von-Esmarch-Straße 56, Münster D-48149, Germany [2] Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Esch-Belval, Luxembourg.
authors.forename: L
authors.initials: L
authors.lastname: Bahnassawy
authors.affiliation: Department of Behavioural Biology, Westfälische Wilhelms-Universität Münster, Badestrasse 13, Muenster D-48149, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Lewejohann
authors.affiliation: Westfälische Wilhelms-Universität Münster, ZMBE, Institute of Cell Biology, Stem Cell Biology and Regeneration Group, Von-Esmarch-Straße 56, Münster D-48149, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Palm
authors.affiliation: 1] Westfälische Wilhelms-Universität Münster, ZMBE, Institute of Cell Biology, Stem Cell Biology and Regeneration Group, Von-Esmarch-Straße 56, Münster D-48149, Germany [2] Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Esch-Belval, Luxembourg.
authors.forename: J C
authors.initials: JC
authors.lastname: Schwamborn
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D045744:Cell Line, Tumor; D007150:Immunohistochemistry; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: C514011:TRIM32 protein, mouse; D044767:Ubiquitin-Protein Ligases