title: [Study of single nucleotide polymorphism of SOCS gene in typical myeloproliferative neoplasms].
abstract: This study was purposed to investigate the effect of mutation and single nucleotide polymorphism (SNP) of suppressor of cytokine signaling (SOCS) on the typical myeloproliferative neoplasms (MPN) and its mechanism. The mutation and SNP of SOCS1, SOCS2, SOCS3 genes in 100 MPN patients were detected by RT-PCR and direct sequencing. The results showed that among 100 cases there were 21 cases with A→C polymorphism in the 63th site nucleotide of the 15 SOCS3 exon (SNP library no reported), 18 cases with A→C polymorphism in the 1779th site nucleotide of the 15 SOCS3 exon, 49 cases with A→G polymorphism in the 2249th site nucleotide of the 15 SOCS3 exon (SNP library no reported), 39 cases with T→C polymorphism in the 2366th site nucleotide of the 15 SOCS3 exon (SNP library no reported), 9 cases with T→C polymorphism in the exon of 15 SOCS2 gene (SNP library no reported). SOCS3 SNP was found in patients with significantly advanced age at diagnosis, the leukocyte count and platelet level were higher than those in patients with wild type, JAK2V617 mutations was found in 87.65% SOCS3 SNP. It is concluded that the SOCS may be an important target for anticancer therapy, the single nucleotide polymorphism of SOCS may involve to pathogenesis of MPN.
authors.affiliation: Department of Hematology, The Second Hospital of Changzhou, Nanjing Medical University, Changzhou 213000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qin
authors.affiliation: Department of Hematology, The Second Hospital of Changzhou, Nanjing Medical University, Changzhou 213000, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Hematology, The Second Hospital of Changzhou, Nanjing Medical University, Changzhou 213000, China.
authors.forename: Jian-He
authors.initials: JH
authors.lastname: Yang
authors.affiliation: Department of Hematology, The Second Hospital of Changzhou, Nanjing Medical University, Changzhou 213000, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Hematology, The Second Hospital of Changzhou, Nanjing Medical University, Changzhou 213000, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Xiao
authors.affiliation: Department of Hematology, The Second Hospital of Changzhou, Nanjing Medical University, Changzhou 213000, China.
authors.forename: Jing-Tao
authors.initials: JT
authors.lastname: Lu
authors.affiliation: Department of Hematology, The Second Hospital of Changzhou, Nanjing Medical University, Changzhou 213000, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Hematology, The Second Hospital of Changzhou, Nanjing Medical University, Changzhou 213000, China. E-mail:zhoumin@medmail.com.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Hematology, The Second Hospital of Changzhou, Nanjing Medical University, Changzhou 213000, China.
authors.forename: Jin-Yuan
authors.initials: JY
authors.lastname: He
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005091:Exons; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009196:Myeloproliferative Disorders / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D000071222:Suppressor of Cytokine Signaling 1 Protein; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics*; D055815:Young Adult
chemical_list: C108944:SOCS1 protein, human; C106643:SOCS2 protein, human; C106644:SOCS3 protein, human; D000071222:Suppressor of Cytokine Signaling 1 Protein; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins