title: Changes of CD4+CD25+FOXP3+ and CD8+CD28- regulatory T cells in non-small cell lung cancer patients undergoing surgery.
abstract: Little is known about the regulatory T cells (Tregs) in the peripheral blood after surgery of non-small cell lung cancer (NSCLC) patients. In this study, we investigated whether CD4+CD25+FOXP3+ and CD8+CD28- regulatory T cells are decreased in the peripheral blood of NSCLC patients undergoing surgery. The study group (n = 49) comprised NSCLC, and the control group (n = 24) consisted of age- and sex-matched nonmalignant diseases. The prevalence of CD4+CD25+FOXP3+ and CD8+CD28- Tregs was analyzed using flow cytometry. The study group showed significantly higher percentage of CD4+CD25+FOXP3+ and CD8+CD28- Tregs than control. The percentage of CD4+CD25+FOXP3+ and CD8+CD28- Tregs increased with tumor stage. One way ANOVA test shows the significant differences between all subgroups. LSD test shows that there was a statistical significance between each of the two subgroups except stage II in CD4+CD25+FOXP3+ Tregs and control vs. each stage, stage I vs. stage III, and stage IV in CD8+CD28- Tregs. There is no significant difference among stages II, III, and IV in CD8+CD28- Tregs. No differences were found between squamous carcinoma and adenocarcinoma. These levels were dropped significantly after operation. Furthermore postoperative Treg percentage in the early stages (stage I and stage II) was not statistically different from that of controls. Postoperative Treg percentage in advanced stage (III+IV) remained above the values shown by controls. Our findings indicate that the percentage of CD4+CD25+FOXP3+ and CD8+CD28- Tregs correlated with the pathological stage in NSCLC and tumor burden.
authors.affiliation: Department of Cardio-thoracic Surgery, Zhoushan Hospital, No. 739 Dingshen Road, Zhoushan, Zhejiang 316000, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Chen
authors.affiliation: Joint Laboratory of Immunogenomics, Zhoushan Hospital, No. 739 Dingshen Road, Zhoushan, Zhejiang 316000, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Cardio-thoracic Surgery, Zhoushan Hospital, No. 739 Dingshen Road, Zhoushan, Zhejiang 316000, China.
authors.forename: Yongkui
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Cardio-thoracic Surgery, Zhoushan Hospital, No. 739 Dingshen Road, Zhoushan, Zhejiang 316000, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Joint Laboratory of Immunogenomics, Zhoushan Hospital, No. 739 Dingshen Road, Zhoushan, Zhejiang 316000, China.
authors.forename: Wangyu
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of Cardio-thoracic Surgery, Zhoushan Hospital, No. 739 Dingshen Road, Zhoushan, Zhejiang 316000, China.
authors.forename: Binjie
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Cardio-thoracic Surgery, Zhoushan Hospital, No. 739 Dingshen Road, Zhoushan, Zhejiang 316000, China.
authors.forename: Zhaoye
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Cardio-thoracic Surgery, Zhoushan Hospital, No. 739 Dingshen Road, Zhoushan, Zhejiang 316000, China. Electronic address: zslehanbo@163.com.
authors.forename: Hanbo
authors.initials: H
authors.lastname: Le
mesh_terms: D000368:Aged; D018106:CD28 Antigens / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D002289:Carcinoma, Non-Small-Cell Lung / Q000276:immunology* / Q000473:pathology / Q000601:surgery; D005260:Female; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D008175:Lung Neoplasms / Q000276:immunology* / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D018106:CD28 Antigens; D015704:CD4 Antigens; D016827:CD8 Antigens; C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit
keywords: CD4+CD25+FOXP3+; CD8+CD28−; NSCLC; Surgery; Treg