title: Heat shock and structural proteins associated with meat tenderness in Nellore beef cattle, a Bos indicus breed.
abstract: Nellore beef cattle, a Bos indicus (Zebu) breed, is well adapted to tropical conditions and has allowed Brazil to become one of the largest producers of red meat. Nevertheless, B. indicus breeds are reported to have less tender meat than Bos taurus. This study was designed to identify genes associated with meat tenderness and thus provides important information for breeding programs. A group of 138 animals was evaluated for longissimus thoracis muscle shear force (SF). Animals with the highest and lowest SF values (six animals each) were then selected for protein abundance studies. Samples were subjected to two-dimensional gel electrophoresis (2-DE) followed by peptide sequencing through mass spectrometry (MS) to identify differentially expressed proteins associated with SF values. Seventeen differentially expressed spots were observed (p<0.05) between the two groups. The 13 proteins identified included structural proteins (alpha actin-1, MLC1, MLC3, MLC2F and tropomyosin), related to cell organization (HSPB1 and HSP70), metabolism (beta-LG, ACBD6 and Complex III subunit I) and some uncharacterized proteins. Results confirm the existence of differentially expressed proteins associated with SF, which can lead to a better understanding of mechanisms involved in meat tenderness.
authors.affiliation: Department of Animal Science, "Luiz de Queiroz" College of Agriculture, University of São Paulo, P.O. Box 9, Pádua Dias, 11, Piracicaba, SP, Brazil.
authors.forename: Minos Esperândio
authors.initials: ME
authors.lastname: Carvalho
authors.affiliation: Department of Animal Science, "Luiz de Queiroz" College of Agriculture, University of São Paulo, P.O. Box 9, Pádua Dias, 11, Piracicaba, SP, Brazil.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Gasparin
authors.affiliation: Department Basic Science, The Faculty of Animal Science and Food Engineering, University of São Paulo, P.O. Box 23, 225, Duque de Caxias, Pirassununga, SP, Brazil.
authors.forename: Mirele Daiana
authors.initials: MD
authors.lastname: Poleti
authors.affiliation: Department Basic Science, The Faculty of Animal Science and Food Engineering, University of São Paulo, P.O. Box 23, 225, Duque de Caxias, Pirassununga, SP, Brazil.
authors.forename: Alessandra Fernandes
authors.initials: AF
authors.lastname: Rosa
authors.affiliation: Department Basic Science, The Faculty of Animal Science and Food Engineering, University of São Paulo, P.O. Box 23, 225, Duque de Caxias, Pirassununga, SP, Brazil.
authors.forename: Júlio Cesar Carvalho
authors.initials: JC
authors.lastname: Balieiro
authors.affiliation: Department of Genetics, "Luiz de Queiroz" College of Agriculture, University of São Paulo, P.O. Box 13418-900, Pádua Dias, Piracicaba, SP, Brazil.
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Labate
authors.affiliation: Embrapa Southeast Livestock, P.O. Box 339, Rodovia Washington Luiz, km 234, São Carlos, SP, Brazil.
authors.forename: Renata Tieko
authors.initials: RT
authors.lastname: Nassu
authors.affiliation: Embrapa Southeast Livestock, P.O. Box 339, Rodovia Washington Luiz, km 234, São Carlos, SP, Brazil.
authors.forename: Rymer Ramiz
authors.initials: RR
authors.lastname: Tullio
authors.affiliation: Embrapa Southeast Livestock, P.O. Box 339, Rodovia Washington Luiz, km 234, São Carlos, SP, Brazil.
authors.forename: Luciana Correia de Almeida
authors.initials: LC
authors.lastname: Regitano
authors.affiliation: Department of Animal Science, "Luiz de Queiroz" College of Agriculture, University of São Paulo, P.O. Box 9, Pádua Dias, 11, Piracicaba, SP, Brazil.
authors.forename: Gerson Barreto
authors.initials: GB
authors.lastname: Mourão
authors.affiliation: Department of Animal Science, "Luiz de Queiroz" College of Agriculture, University of São Paulo, P.O. Box 9, Pádua Dias, 11, Piracicaba, SP, Brazil. Electronic address: llcoutinho@usp.br.
authors.forename: Luiz Lehmann
authors.initials: LL
authors.lastname: Coutinho
mesh_terms: D000818:Animals; D001938:Brazil; D001947:Breeding; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006358:Hot Temperature; D008297:Male; D008460:Meat / Q000032:analysis*; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000737:chemistry*; D010766:Phosphorylation
chemical_list: D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D009124:Muscle Proteins
keywords: Bos indicus; Proteomics; Shear force; Tenderness