title: Quantitative analysis of axonal transport by using compartmentalized and surface micropatterned culture of neurons.
abstract: Mitochondria, synaptic vesicles, and other cytoplasmic constituents have to travel long distance along the axons from cell bodies to nerve terminals. Interruption of this axonal transport may contribute to many neurodegenerative diseases including Alzheimer's disease (AD). It has been recently shown that exposure of cultured neurons to β-amyloid (Aβ) resulted in severe impairment of mitochondrial transport. This Letter describes an integrated microfluidic platform that establishes surface patterned and compartmentalized culture of neurons for studying the effect of Aβ on mitochondria trafficking in full length of axons. We have successfully quantified the trafficking of fluorescently labeled mitochondria in distal and proximal axons using image processing. Selective treatment of Aβ in the somal or axonal compartments resulted in considerable decrease in mitochondria movement in a location dependent manner such that mitochondria trafficking slowed down more significantly proximal to the location of Aβ exposure. Furthermore, this result suggests a promising application of microfluidic technology for investigating the dysfunction of axonal transport related to neurodegenerative diseases.
authors.forename: Hyung Joon
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jeong Won
authors.initials: JW
authors.lastname: Park
authors.forename: Jae Hwan
authors.initials: JH
authors.lastname: Byun
authors.forename: Wayne W
authors.initials: WW
authors.lastname: Poon
authors.forename: Carl W
authors.initials: CW
authors.lastname: Cotman
authors.forename: Charless C
authors.initials: CC
authors.lastname: Fowlkes
authors.forename: Noo Li
authors.initials: NL
authors.lastname: Jeon
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000378:metabolism; D000818:Animals; D001370:Axonal Transport / Q000502:physiology*; D002451:Cell Compartmentation / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007734:Kymography / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D051336:Mitochondrial Membranes / Q000378:metabolism* / Q000473:pathology; D009474:Neurons / Q000378:metabolism* / Q000473:pathology / Q000502:physiology; D051381:Rats
chemical_list: D016229:Amyloid beta-Peptides