title: Vibrio cholerae O1 epidemic variants in Angola: a retrospective study between 1992 and 2006.
abstract: Cholera is still a major public health concern in many African countries. In Angola, after a decade of absence, cholera reemerged in 1987, spreading throughout the country until 1996, with outbreaks recurring in a seasonal pattern. In 2006 Angola was hit by one of the most severe outbreaks of the last decade, with ca. 240,000 cases reported. We analyzed 21 clinical strains isolated between 1992 and 2006 from several provinces throughout the country: Benguela, Bengo, Luanda, Cuando Cubango, and Cabinda. We used two multiplex PCR assays to investigate discriminatory mobile genetic elements (MGE) [Integrative Conjugative Elements (ICEs), VSP-II, GI12, GI14, GI15, K, and TLC phages] and we compared the profiles obtained with those of different reference V. cholerae O1 variants (prototypical, altered, and hybrid), responsible for the ongoing 7th pandemic. We also tested the strains for the presence of specific VSP-II variants and for the presence of a genomic island (GI) (WASA-1), correlated with the transmission of seventh pandemic cholera from Africa to South America. Based on the presence/absence of the analyzed genetic elements, five novel profiles were detected in the epidemic strains circulating in the 1990s. The most frequent profiles, F and G, were characterized by the absence of ICEs and the three GIs tested, and the presence of GI WASA-1 and the WASA variant of the VSP-II island. Our results identified unexpected variability within the 1990s epidemic, showing different rearrangements in a dynamic part of the genome not present in the prototypical V. cholerae O1 N16961. Moreover the 2006 strains differed from the current pandemic V. cholerae O1 strain. Taken together, our results highlight the role of horizontal gene transfer (HGT) in diversifying the genetic background of V. cholerae within a single epidemic.
authors.affiliation: Dipartimento di Biologia e Biotecnologie C. Darwin, Università di Roma Sapienza Rome, Italy.
authors.forename: Romy
authors.initials: R
authors.lastname: Valia
authors.affiliation: Dipartimento di Biologia e Biotecnologie C. Darwin, Università di Roma Sapienza Rome, Italy ; Centro de Biotecnologia, Universidade E. Mondlane Maputo Mozambique.
authors.forename: Elisa
authors.initials: E
authors.lastname: Taviani
authors.affiliation: Department of Genetics, University College London Genetics Institute, Evolution and Environment, University College London London, UK.
authors.forename: Matteo
authors.initials: M
authors.lastname: Spagnoletti
authors.affiliation: Department of Cell Biology and Molecular Genetics, Maryland Pathogen Research Institute, University of Maryland College Park, MD, USA.
authors.forename: Daniela
authors.initials: D
authors.lastname: Ceccarelli
authors.affiliation: Dipartimento di Scienze Biomediche, Università di Sassari Sassari, Italy.
authors.forename: Piero
authors.initials: P
authors.lastname: Cappuccinelli
authors.affiliation: Dipartimento di Biologia e Biotecnologie C. Darwin, Università di Roma Sapienza Rome, Italy ; Centro de Biotecnologia, Universidade E. Mondlane Maputo Mozambique.
authors.forename: Mauro M
authors.initials: MM
authors.lastname: Colombo
keywords: Angola; V. cholerae O1; epidemic variants; genomic islands; mobilome