title: [Preparation of thrombin affinity chromatography media].
abstract: The preparation of thrombin affinity chromatography media is described in this paper. First, the thrombin affinity chromatography media was prepared through thrombin coupled with cyanogen bromide activated agarose. And then the enzyme activity of thrombin on the coupled media was measured by chromogenic substrate method. The conditions were confirmed based on the enzyme activity of thrombin on the coupled media. The optimum conditions were as follows: the use of Na2CO3-NaHCO3 (containing 0.5 mol/L NaCl) solution of pH 8.3 as the reaction solution, 200 U thrombin per 1 g agarose, and reaction for 10 h at room temperature. The prepared affinity chromatography media had good stability, on which 70.6% of the enzyme activity of thrombin existed after 40 days' storage. The thrombin affinity chromatography media can be widely applied in the screening and purification of thrombin inhibitors in natural products.
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Xiongfei
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ye
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Luo
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Zhan
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Ruijuan
authors.initials: R
authors.lastname: Yuan
mesh_terms: D002846:Chromatography, Affinity; D012685:Sepharose; D013917:Thrombin
chemical_list: D012685:Sepharose; D013917:Thrombin