title: Optimisation of microencapsulation of holy basil essential oil in gelatin by response surface methodology.
abstract: To protect holy basil essential oil (HBEO) from volatilisation and oxidation, microencapsulation by simple coacervation of gelatin was developed. An optimal encapsulating condition obtained from response surface methodology (RSM) was a gelatin concentration of 11.75% (w/v) and an HBEO amount of 31ml, which provided the greatest yield, oil content, and encapsulation efficiency of 98.80%, 66.50%, and 95.41%, respectively. Scanning electron microscope (SEM) revealed that the internal surface of the microcapsule was honeycomb-like networks containing nonhomogeneous distributions of HBEO. Fourier transform infrared spectroscopy (FTIR) indicated that there was no significant interaction between the HBEO and gelatin. Under storage conditions at 60°C for 49days equivalent to 25°C for 18months, small decreases in the HBEO retention rate and the antioxidant activity were observed. Thus, the microencapsulated formulation has potential to be applied to other volatile compounds.
authors.affiliation: Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.
authors.forename: Polin
authors.initials: P
authors.lastname: Sutaphanit
authors.affiliation: Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand. Electronic address: pakamon.c@ku.ac.th.
authors.forename: Pakamon
authors.initials: P
authors.lastname: Chitprasert
mesh_terms: D002214:Capsules / Q000737:chemistry; D005524:Food Technology / Q000379:methods*; D005780:Gelatin / Q000737:chemistry*; D018649:Ocimum basilicum / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*
chemical_list: D002214:Capsules; D009822:Oils, Volatile; D010938:Plant Oils; D005780:Gelatin
keywords: Essential oil; Gelatin; Holy basil; Microencapsulation; Optimisation; Response surface methodology