title: p38 and extracellular signal-regulated kinases activations have opposite effects on primary-cultured rat cerebellar granule neurons exposed to sodium arsenite.
abstract: Arsenic is a widespread environmental toxicant in the world and regarded as both a carcinogen and an anticarcinogen. The present study was designed to evaluate roles of mitogen-activated protein kinases in sodium arsenite-induced effects on primary-cultured rat cerebellar granule neurons (CGNs). Results revealed a decreased viability of the cells exposed to sodium arsenite (from 0 to 50 μM) in a dose-dependent manner. Annexin V-fluorescein isothiocyanate assay showed that apoptosis was obviously induced by arsenite treatment. High phosphorylation expressions of p38 and extracellular signal-regulated kinases (ERK1/2), but not of c-Jun N-terminal kinases were observed due to arsenite treatment by western blotting analysis. Furthermore, SB203580 (an inhibitor of p38) decreased the percentage of apoptotic cells whereas arsenite-stimulated toxicity was enhanced by U0126 (an inhibitor of ERK1/2). Taken together, these data suggest that p38 contributes to arsenite-induced apoptosis of rat CGNs, but ERK1/2 may involve in cell growth and survival.
authors.affiliation: Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province and Ministry of Health, Harbin, 150081, People's Republic of China.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Liu
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Gao
authors.forename: Hongju
authors.initials: H
authors.lastname: Yao
authors.forename: Lingwang
authors.initials: L
authors.lastname: Zhou
authors.forename: Junrui
authors.initials: J
authors.lastname: Pei
authors.forename: Liyan
authors.initials: L
authors.lastname: Sun
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Dianjun
authors.initials: D
authors.lastname: Sun
mesh_terms: D000818:Animals; D017209:Apoptosis; D001149:Arsenates / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology*; D004785:Environmental Pollutants / Q000633:toxicity*; D004789:Enzyme Activation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D009474:Neurons / Q000187:drug effects / Q000201:enzymology*; D061251:Primary Cell Culture; D017208:Rats, Wistar; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D001149:Arsenates; D004785:Environmental Pollutants; C009277:sodium arsenate; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Mitogen-Activated Protein Kinases; Rat Cerebellar Granule Neurons; Sodium Arsenite