title: Development and validation of a lateral flow assay for the detection of crustacean protein in processed foods.
abstract: We developed and validated a novel lateral flow assay for the detection of crustacean protein in processed foods. This assay had high sensitivity; the visual detection limit for shrimp protein extract was 25μg/L, equivalent to 1μg/g protein in a food sample, and results could be obtained within 20min without sophisticated procedures or expensive equipment. Concordance between our assay and another validated quantitative enzyme-linked immunosorbent assay was 97% for commercially processed foods. This assay is rapid, simple, reliable, and highly correlated with validated enzyme-linked immunosorbent assays and is thus suitable for monitoring of food products, especially in food-processing facilities.
authors.affiliation: Central Research Institute, Maruha Nichiro Holdings, Inc., 16-2 Wadai, Tsukuba, Ibaraki 300-4295, Japan. Electronic address: d-koizumi@maruha-nichiro.co.jp.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Koizumi
authors.affiliation: Central Research Institute, Maruha Nichiro Holdings, Inc., 16-2 Wadai, Tsukuba, Ibaraki 300-4295, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Shirota
authors.affiliation: Central Research Institute, Maruha Nichiro Holdings, Inc., 16-2 Wadai, Tsukuba, Ibaraki 300-4295, Japan.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Akita
authors.affiliation: Central Research Institute, Maruha Nichiro Holdings, Inc., 16-2 Wadai, Tsukuba, Ibaraki 300-4295, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Oda
authors.affiliation: National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Akiyama
mesh_terms: D000485:Allergens / Q000032:analysis / Q000276:immunology; D000818:Animals; D060829:Arthropod Proteins / Q000032:analysis* / Q000276:immunology; D003445:Crustacea / Q000737:chemistry* / Q000276:immunology; D057140:Fast Foods / Q000032:analysis; D005506:Food Contamination / Q000032:analysis*; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection
chemical_list: D000485:Allergens; D060829:Arthropod Proteins
keywords: CV; Crustacean; Enzyme immunoassay; Food allergy; Lateral flow assay; MS; coefficients of variation; mABS; mass spectrometry; milli absorbance