title: [Microeukaryotic biodiversity in the waste ore samples surrounding an acid mine drainage lake].
abstract: The abandoned mineral samples were collected in an acid mine drainage area in Anhui Province. Molecular ecological methods were used to construct 18S rDNA clone libraries after analyzing the main physicochemical parameters, and then the microeukaryotic diversity and community structure in the acid mine drainage area were studied. The results showed that the region was strongly acidic (pH <3), and the concentrations of Fe, SO2-(4), P, NO-(3) -N showed the same trend, all higher in the bare waste ore samples PD and 1 M than in the vegetation covered samples LW and XC. Four eukaryotic phyla were detected in the abandoned mineral samples: Ascomycota, Basidiomycota, Glomeromycota and Arthropoda. Glomeromycota can form an absolute symbiotic relationship with the plant, and it was a key factor for early plant to adapt the terrestrial environment. The biodiversity of the vegetation covered samples LW and XC, which contained Glomeromycota, was much higher than that of the bare abandoned rock samples PD and 1 M. Moreover, many sequences in the libraries were closely related to some isolated strains, which are tolerant to low pH and heavy metals, such as Penicillium purpurogenum, Chaetothyriales sp. and Staninwardia suttonii.
authors.affiliation: Key Laboratory of Groundwater Circulation and Evolution of Ministry of Education, China University of Geosciences, Beijing 100083, China. lisiyuan236@163.com
authors.forename: Si-Yuan
authors.initials: SY
authors.lastname: Li
authors.affiliation: Key Laboratory of Groundwater Circulation and Evolution of Ministry of Education, China University of Geosciences, Beijing 100083, China.
authors.forename: Chun-Bo
authors.initials: CB
authors.lastname: Hao
authors.affiliation: Key Laboratory of Groundwater Circulation and Evolution of Ministry of Education, China University of Geosciences, Beijing 100083, China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Wang
authors.affiliation: Shijiazhuang Iron & Steel Co., Ltd., (Xinyue Sintering), Shijiazhuang 050033, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Lü
authors.affiliation: Key Laboratory of Groundwater Circulation and Evolution of Ministry of Education, China University of Geosciences, Beijing 100083, China.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Groundwater Circulation and Evolution of Ministry of Education, China University of Geosciences, Beijing 100083, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Groundwater Circulation and Evolution of Ministry of Education, China University of Geosciences, Beijing 100083, China.
authors.forename: Chuan-Ping
authors.initials: CP
authors.lastname: Feng
mesh_terms: D000143:Acids / Q000032:analysis; D000818:Animals; D001181:Arthropods / Q000145:classification; D001203:Ascomycota / Q000145:classification; D001487:Basidiomycota / Q000145:classification; D044822:Biodiversity; D004275:DNA, Ribosomal; D055137:Glomeromycota / Q000145:classification; D060106:Lakes / Q000737:chemistry*; D019216:Metals, Heavy / Q000032:analysis*; D008906:Mining; D010944:Plants; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D000143:Acids; D004275:DNA, Ribosomal; D019216:Metals, Heavy; D012337:RNA, Ribosomal, 18S