title: The effects of dietary restriction and aging on amyloid precursor protein and presenilin-1 mRNA and protein expression in rat brain.
abstract: The objective of this study was to examine the effects of aging and long-term dietary restriction (DR) on the level of amyloid precursor protein (APP) and presenilin-1 (PS-1), proteins that are critically involved in Alzheimer's disease. Changes in mRNA and protein expression were assessed by real-time PCR and western blot analysis during aging and long-term DR in the cortex and hippocampus of 6-, 12-, 18-, and 24-month-old rats. Prominent regional changes in expression were observed in response to aging and DR. Although the hippocampus displayed significant alterations in APP mRNA and protein expression and no significant changes in PS-1 expression, an opposite pattern was observed in the cortex. DR counteracted the age-related changes in APP mRNA expression in both structures of old animals. The observed DR-induced increase in mRNA levels in the hippocampus was accompanied by an increase in the level of full-length protein APP. These results indicate that although both structures are very sensitive to aging, a specific spatial pattern of changes in APP and PS-1 occurs during aging. Furthermore, these findings provide evidence that DR can affect APP and PS-1 expression in a manner consistent with its proposed 'antiaging' effect.
authors.affiliation: Department of Neurobiology, Institute for Biological Research 'Sinisa Stankovic', University of Belgrade, Belgrade, Serbia.
authors.forename: Aleksandra N
authors.initials: AN
authors.lastname: Mladenovic Djordjevic
authors.forename: Milka
authors.initials: M
authors.lastname: Perovic
authors.forename: Kosara
authors.initials: K
authors.lastname: Smiljanic
authors.forename: Smilja
authors.initials: S
authors.lastname: Todorovic
authors.forename: Vesna
authors.initials: V
authors.lastname: Tesic
authors.forename: Sabera
authors.initials: S
authors.lastname: Ruzdijic
authors.forename: Selma
authors.initials: S
authors.lastname: Kanazir
mesh_terms: D000375:Aging / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000096:biosynthesis*; D000818:Animals; D031204:Caloric Restriction; D002540:Cerebral Cortex / Q000378:metabolism* / Q000473:pathology; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D008297:Male; D053764:Presenilin-1 / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016564:Amyloid beta-Protein Precursor; D053764:Presenilin-1; D012333:RNA, Messenger