title: Identification and characterization of microRNAs in the crab-eating macaque (Macaca fascicularis) using transcriptome analysis.
abstract: MicroRNAs (miRNAs), with an average length between 16 nt and 26 nt, are small non-coding RNAs that can repress gene expression on the post-transcriptional level. Macaca fascicularis (M. fascicularis), one of the most important nonhuman primate animal models, is widely used in basic and applied preclinical research, especially in studies that involve neuroscience and disease. However, due to the lack of a complete genome sequence, the miRNAs in M. fascicularis have not been completely characterized. In this study, 86 putative M. fascicularis miRNAs were identified using a strategy of our design. The expression of some of these miRNAs in the tissue was confirmed by qRT-PCR. The function and pathway of their targeted genes were analyzed to reveal the potential relevance of miRNA regulation on diseases and physiological processes. The current study provides insight into potential miRNAs and forms a useful knowledge base for the future understanding of the function of miRNAs in M. fascicularis.
authors.affiliation: Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Yang
authors.affiliation: Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Jing
authors.affiliation: Institute of Discovery Biology, Jiangsu Simcere Pharmaceutical R&D Co., Ltd, 699-18 Xuan Wu Avenue, Nanjing 210042, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093, China. Electronic address: kzen@nju.edu.cn.
authors.forename: Ke
authors.initials: K
authors.lastname: Zen
authors.affiliation: Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093, China. Electronic address: cyzhang@nju.edu.cn.
authors.forename: Chenyu
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093, China. Electronic address: donghaili@nju.edu.cn.
authors.forename: Donghai
authors.initials: D
authors.lastname: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D015870:Gene Expression / Q000235:genetics*; D020869:Gene Expression Profiling; D008252:Macaca fascicularis / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D015398:Signal Transduction / Q000235:genetics; D059467:Transcriptome / Q000235:genetics
chemical_list: D035683:MicroRNAs
keywords: B lymphoma Mo-MLV insertion region 1 homolog; BMI1; CT; DAVID; DEPC; Database for Annotation, Visualization and Integrated Discovery; ES; Ezh2; FGF; HF; M. fascicularis; MFE; MFEI; Macaca fascicularis; MicroRNA; PBS; RCC; SMC; SPRED-1; Sprouty-Related, EVH1 Domain Containing 1; Transcriptome; VEGF; ZEB2; cycle threshold; diethylpyrocarbonate; embryonic stem; enhancer of zeste homolog 2; fibroblast growth factor; heart failure; miRNAs; microRNAs; minimal folding free energy; minimal folding free energy index; phosphate-buffered saline; renal cell carcinoma; skeletal muscle cell; vascular endothelial growth factor; zinc finger E-box binding homeobox 2