title: Selective extraction from microalgae Nannochloropsis sp. using different methods of cell disruption.
abstract: This work studies the extraction of intracellular components from microalgae Nannochloropsis sp. with application of different cell disruption techniques, including pulsed electric field (PEF) (20kV/cm, 1-4ms, 13.3-53.1kJ/kg), high voltage electrical discharge (HVED) (40kV/cm, 1-4ms, 13.3-53.1kJ/kg), ultrasonication (USN) (200W, 1-8min, 12-96kJ/kg), and high pressure homogenization (HPH) (150MPa, 1-10 passes, 150-1500kJ/kg). The data evidence that electrically based disruption techniques (PEF and HVED) allowed selective extraction of water soluble ionic components and microelements, small molecular weight organic compounds and water soluble proteins. Microscopic and sedimentation stability analyses have shown that microalgae cells in HVED-treated suspension were noticeably agglomerated and could be easily settled in centrifuge. The electrically based disruption techniques were ineffective for delivery of pigments (e.g., chlorophylls or carotenoids) and their extraction required subsequent application of more potent disruption techniques. The obtained data have shown that HPH disruption technique was the most effective; however, this mode required the highest power consumption.
authors.affiliation: Université de Technologie de Compiègne, Département de Génie des procédés industriels, Unité Transformations Intégrées de la Matière Renouvelable (UTC/ESCOM, EA 4297 TIMR), Rue Roger Couttolenc, CS 60319 Compiègne Cedex, France. Electronic address: nabil.grimi@utc.fr.
authors.forename: N
authors.initials: N
authors.lastname: Grimi
authors.affiliation: Université de Technologie de Compiègne, Département de Génie des procédés industriels, Unité Transformations Intégrées de la Matière Renouvelable (UTC/ESCOM, EA 4297 TIMR), Rue Roger Couttolenc, CS 60319 Compiègne Cedex, France; LUNAM Université, CNRS, GEPEA, Université de Nantes, UMR6144, CRTT, Boulevard de l'Université, BP 406, 44602 Saint-Nazaire Cedex, France.
authors.forename: A
authors.initials: A
authors.lastname: Dubois
authors.affiliation: LUNAM Université, CNRS, GEPEA, Université de Nantes, UMR6144, CRTT, Boulevard de l'Université, BP 406, 44602 Saint-Nazaire Cedex, France.
authors.forename: L
authors.initials: L
authors.lastname: Marchal
authors.affiliation: AlgoSource Technologies, 37 Bd de l'Université, 44600 Saint-Nazaire, France.
authors.forename: S
authors.initials: S
authors.lastname: Jubeau
authors.affiliation: Université de Technologie de Compiègne, Département de Génie des procédés industriels, Unité Transformations Intégrées de la Matière Renouvelable (UTC/ESCOM, EA 4297 TIMR), Rue Roger Couttolenc, CS 60319 Compiègne Cedex, France; Institute of Biocolloidal Chemistry named after F.D. Ovcharenko, NAS of Ukraine, 42, blvr. Vernadskogo, Kyiv 03142, Ukraine.
authors.forename: N I
authors.initials: NI
authors.lastname: Lebovka
authors.affiliation: Université de Technologie de Compiègne, Département de Génie des procédés industriels, Unité Transformations Intégrées de la Matière Renouvelable (UTC/ESCOM, EA 4297 TIMR), Rue Roger Couttolenc, CS 60319 Compiègne Cedex, France.
authors.forename: E
authors.initials: E
authors.lastname: Vorobiev
mesh_terms: D000042:Absorption; D020418:Algal Proteins / Q000302:isolation & purification; D002458:Cell Fractionation / Q000379:methods*; D002498:Centrifugation; D004553:Electric Conductivity; D008027:Light; D058086:Microalgae / Q000302:isolation & purification*; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet
chemical_list: D020418:Algal Proteins
keywords: Electrotechnologies; HPH; HVED; High pressure homogenization; Microalgae Nannochloropsis sp.; PEF; Selective extraction; U; USN; Ultrasonication; high pressure homogenization; high voltage electric discharge; pulsed electric field; ultra-sonication; untreated