title: Effects of tetrahydropalmatine on post-traumatic stress disorder-induced changes in rat brain gene expression.
abstract: The aim of this research proposal was to investigate the effects of tetrahydropalmatine (THP) on gene expression in a post-traumatic stress disorder (PTSD) rodent model. Eighty male Sprague-Dawley rats were randomly assigned to the nonstressed groups or the 3-day restraint shock PTSD rodent model groups. There were four groups within the nonstressed rats (control, THP, midazolam, and midazolam with THP) and four groups within the stressed rats (control, THP, midazolam, and midazolam with THP). After injection the subjects were euthanized and the amygdala and hippocampus were sent for reverse transcriptase-polymerase chain reaction gene expression analysis. Of the genes interrogated, 17 genes in the amygdala and 18 genes in the hippocampus were found to have significant changes in gene expression and regulation. Significant transcriptional fold changes were found in important genes involved in dopamine, serotonin, acetylcholine, and gamma-aminobutyric acid neurotransmitter systems. The results provide quantifiable data demonstrating gene expression changes in PTSD-stressed and nonstressed rats receiving various treatments. These findings contribute important data to the limited molecular details pertaining to the neurobiology of PTSD.
authors.affiliation: US Army Graduate Program, 3490 Forage Road; Suite 112, Fort Sam, Houston Texas 78234-7585, USA.
authors.forename: T E
authors.initials: TE
authors.lastname: Ceremuga
authors.forename: P
authors.initials: P
authors.lastname: Shellabarger
authors.forename: T
authors.initials: T
authors.lastname: Persson
authors.forename: M
authors.initials: M
authors.lastname: Fanning
authors.forename: P
authors.initials: P
authors.lastname: Galey
authors.forename: D
authors.initials: D
authors.lastname: Robinson
authors.forename: S
authors.initials: S
authors.lastname: Bertsch
authors.forename: G A
authors.initials: GA
authors.lastname: Ceremuga
authors.forename: M
authors.initials: M
authors.lastname: Bentley
mesh_terms: D000759:Adjuvants, Anesthesia / Q000494:pharmacology / Q000627:therapeutic use; D018712:Analgesics, Non-Narcotic / Q000494:pharmacology* / Q000627:therapeutic use; D000704:Analysis of Variance; D000818:Animals; D001600:Berberine Alkaloids / Q000494:pharmacology* / Q000627:therapeutic use; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects*; D008297:Male; D008874:Midazolam / Q000494:pharmacology / Q000627:therapeutic use; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D017981:Receptors, Neurotransmitter / Q000235:genetics / Q000378:metabolism*; D013313:Stress Disorders, Post-Traumatic / Q000188:drug therapy / Q000473:pathology*
chemical_list: D000759:Adjuvants, Anesthesia; D018712:Analgesics, Non-Narcotic; D001600:Berberine Alkaloids; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D017981:Receptors, Neurotransmitter; C014215:tetrahydropalmatine; D008874:Midazolam