title: Different responses of human mononuclear phagocyte populations to Mycobacterium tuberculosis.
abstract: Mycobacterium tuberculosis (Mtb) infects different populations of macrophages. Alveolar macrophages (AMs) are initially infected, and their response may contribute to controlling Mtb infection and dissemination. However, Mtb infection may disseminate to other tissues, infecting a wide variety of macrophages. Given the difficulty in obtaining AMs, monocyte-derived macrophages (MDMs) are used to model macrophage-mycobacteria interactions in humans. However, the response of other tissue macrophages to Mtb infection has been poorly explored. We have compared MDMs, AMs and splenic human macrophages (SMs) for their in vitro capacity to control Mtb growth, cytokine production, and induction of cell death in response to Mtb H37Rv, and the Colombian isolate UT205, and to the virulence factor ESAT-6. Significant differences in the magnitude of cell death and cytokine production depending mainly on the Mtb strain were observed; however, no major differences in the mycobacteriostatic/mycobacteriocidal activity were detected among the macrophage populations. Infection with the clinical isolate UT205 was associated with an increased cell death with membrane damage, particularly in IFNγ-treated SMs and H37Rv induced a higher production of cytokines compared to UT205. These results are concordant with the interpretation of a differential response to Mtb infection mainly depending upon the strain of Mtb.
authors.affiliation: Grupo de Inmunología Celular e Inmunogenética (GICIG), Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia, UdeA, Calle 70 No. 52-21, Medellín, Colombia.
authors.forename: Camilo
authors.initials: C
authors.lastname: Duque
authors.affiliation: Grupo de Inmunología Celular e Inmunogenética (GICIG), Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia, UdeA, Calle 70 No. 52-21, Medellín, Colombia.
authors.forename: Leonar
authors.initials: L
authors.lastname: Arroyo
authors.affiliation: Hospital Pablo Tobón Uribe, Medellín, Colombia; Clínica Cardiovascular Santa María, Medellín, Colombia.
authors.forename: Héctor
authors.initials: H
authors.lastname: Ortega
authors.affiliation: IPS Universitaria Clínica León XIII Sede Medellín, Medellín, Colombia.
authors.forename: Franco
authors.initials: F
authors.lastname: Montúfar
authors.affiliation: Grupo de Inmunología Celular e Inmunogenética (GICIG), Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia, UdeA, Calle 70 No. 52-21, Medellín, Colombia.
authors.forename: Blanca
authors.initials: B
authors.lastname: Ortíz
authors.affiliation: Grupo de Inmunología Celular e Inmunogenética (GICIG), Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia, UdeA, Calle 70 No. 52-21, Medellín, Colombia.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Rojas
authors.affiliation: Grupo de Inmunología Celular e Inmunogenética (GICIG), Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia, UdeA, Calle 70 No. 52-21, Medellín, Colombia. Electronic address: fbarrera@udea.edu.co.
authors.forename: Luis F
authors.initials: LF
authors.lastname: Barrera
mesh_terms: D000328:Adult; D000942:Antigens, Bacterial / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D020382:Interleukin-18 / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008262:Macrophage Activation; D016676:Macrophages, Alveolar / Q000276:immunology*; D008297:Male; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010446:Peptide Fragments / Q000378:metabolism; D010586:Phagocytes; D014376:Tuberculosis / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C093276:ESAT-6 protein, Mycobacterium tuberculosis; D020382:Interleukin-18; D053583:Interleukin-1beta; D015850:Interleukin-6; D010446:Peptide Fragments; C052994:interleukin-1beta (163-171); D007371:Interferon-gamma
keywords: Cell death; Cytokines; ESAT-6; Interferon gamma; Mononuclear phagocytes; Mycobacterium tuberculosis