title: Evidence for A1 and A 3 receptors mediating adenosine-induced intracellular calcium release in the dorsal root ganglion neurons by using confocal microscopy imaging.
abstract: Adenosine exerts a key role in analgesia. In the present study, adenosine-induced Ca(2+) responses were revealed by using confocal microscopy imaging in the rat dorsal root ganglia (DRG) neurons in vitro. Our results showed that adenosine could evoke increases in the intracellular Ca(2+) concentration in the DRG neurons. In addition, by application of selective receptor antagonists, two types of receptors, A1R and A3R, were identified to be involved in the adenosine-induced Ca(2+) release from intracellular stores in neurons. Altogether, these results suggest that confocal microscopy imaging combined with fluorescent dyes could help to detect the analgesic-induced ion signaling in single cell.
authors.affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Collage of Photonic and Electronic Engineering, Fujian Normal University, Fuzhou, 350007, China.
authors.forename: Liqin
authors.initials: L
authors.lastname: Zheng
authors.forename: Jiangxu
authors.initials: J
authors.lastname: Chen
authors.forename: Yimei
authors.initials: Y
authors.lastname: Huang
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Wang
authors.forename: Hongqin
authors.initials: H
authors.lastname: Yang
authors.forename: Yanding
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shusen
authors.initials: S
authors.lastname: Xie
mesh_terms: D000241:Adenosine / Q000494:pharmacology*; D058916:Adenosine A1 Receptor Antagonists / Q000494:pharmacology; D058918:Adenosine A3 Receptor Antagonists / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism*; D018613:Microscopy, Confocal / Q000379:methods*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D061848:Optical Imaging; D017207:Rats, Sprague-Dawley; D043682:Receptor, Adenosine A1 / Q000378:metabolism*; D043684:Receptor, Adenosine A3 / Q000378:metabolism*
chemical_list: D058916:Adenosine A1 Receptor Antagonists; D058918:Adenosine A3 Receptor Antagonists; D043682:Receptor, Adenosine A1; D043684:Receptor, Adenosine A3; D000241:Adenosine; D002118:Calcium