title: Activation of focal adhesion kinase induces extracellular signal-regulated kinase-mediated osteogenesis in tensile force-subjected periodontal ligament fibroblasts but not in osteoblasts.
abstract: The exact mechanism by which focal adhesion kinase (FAK) translates mechanical signals into osteogenesis differentiation in force-subjected cells has not been elucidated. The responses to different forces differ according to the origin of cells and the type of stress applied. Therefore, the recruitment of osteoclast and osteoblast progenitor cells, and the balanced activation of these cells around and within the periodontal ligament (PDL) are essential for alveolar bone remodeling. Cells within the PDL and MG63 cells were subjected to tensile forces of -100 kPa for different periods of time. At various times during the tensile force application, they were processed for the purpose of analyzing cell viability, cell cycle, and osteogenic protein. The effect of small interfering RNA transfection targeting FAK was also evaluated. Tensile force enhanced a rapid increase in the phosphorylation of FAK and up-regulated osteogenic protein expression in PDL cells, but not in MG63 cells. Transfecting PDL cells with FAK antisense oligonucleotide diminished alkaline phosphatase and osteocalcin secretion. These findings suggest that tensile force activates FAK pathways in PDL cells, which down-regulate immune cytokine and up-regulate osteogenic protein.
authors.affiliation: School of Dentistry, Chung Shan Medical University, Taichung, 402, Taiwan.
authors.forename: Yi-Jyun
authors.initials: YJ
authors.lastname: Chen
authors.forename: Ming-You
authors.initials: MY
authors.lastname: Shie
authors.forename: Chi-Jr
authors.initials: CJ
authors.lastname: Hung
authors.forename: Buor-Chang
authors.initials: BC
authors.lastname: Wu
authors.forename: Shiau-Lee
authors.initials: SL
authors.lastname: Liu
authors.forename: Tsui-Hsien
authors.initials: TH
authors.lastname: Huang
authors.forename: Chia-Tze
authors.initials: CT
authors.lastname: Kao
mesh_terms: D002460:Cell Line; D004789:Enzyme Activation / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D051417:Focal Adhesion Kinase 1 / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D020935:MAP Kinase Signaling System / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism*; D013718:Tensile Strength
chemical_list: D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human