title: Effects of isoflurane anesthesia on F-waves in the sciatic nerve of the adult rat.
abstract: BACKGROUND
  Nerve conduction studies provide insights into the functional consequences of axonal and myelin pathology in peripheral neuropathies. We investigated whether isoflurane inhalation anesthesia alters F-wave latencies and F-persistence in the sciatic nerve of adult rats.
  
  
  METHODS
  Ten rats were investigated at 3 different isoflurane concentrations followed by ketamine-xylazine injection anesthesia. To assess F-wave latencies, a stimulation paradigm was chosen to minimize H-reflex masking of F-waves.
  
  
  RESULTS
  F-wave persistence rates were reduced with 3.5% isoflurane concentration at 4 and 10 Hz supramaximal stimulation and marginally reduced with 2.5% isoflurane when compared with ketamine-xylazine. F-wave amplitudes decreased progressively with rising stimulus frequency in all types of anesthesia and most at 3.5% isoflurane concentration.
  
  
  CONCLUSIONS
  The type of anesthesia and the stimulus repetition rate have an impact on some F-wave parameters. Higher isoflurane concentrations and repetition rates are not recommended in experimental studies using rat neuropathy models where F-waves are of interest.
authors.affiliation: Institute of Anatomy, University of Leipzig, Leipzig, Germany.
authors.forename: Marcin
authors.initials: M
authors.lastname: Nowicki
authors.forename: Petra
authors.initials: P
authors.lastname: Baum
authors.forename: Joanna
authors.initials: J
authors.lastname: Kosacka
authors.forename: Maximilian
authors.initials: M
authors.lastname: Stockinger
authors.forename: Nora
authors.initials: N
authors.lastname: Klöting
authors.forename: Matthias
authors.initials: M
authors.lastname: Blüher
authors.forename: Ingo
authors.initials: I
authors.lastname: Bechmann
authors.forename: Klaus V
authors.initials: KV
authors.lastname: Toyka
mesh_terms: D000778:Anesthetics, Dissociative / Q000494:pharmacology; D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D019054:Evoked Potentials, Motor / Q000187:drug effects*; D006181:H-Reflex / Q000187:drug effects*; D007530:Isoflurane / Q000494:pharmacology*; D007649:Ketamine / Q000494:pharmacology; D008297:Male; D009125:Muscle Relaxants, Central / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000187:drug effects*; D018709:Statistics, Nonparametric; D014991:Xylazine / Q000494:pharmacology
chemical_list: D000778:Anesthetics, Dissociative; D018685:Anesthetics, Inhalation; D009125:Muscle Relaxants, Central; D014991:Xylazine; D007649:Ketamine; D007530:Isoflurane
keywords: F-wave; isoflurane; ketamine-xylazine; nerve conduction velocity; sciatic nerve