title: Cell adhesion and invasion inhibitory effect of an ovarian cancer targeting peptide selected via phage display in vivo.
abstract: Organ-specific metastasis is of great importance since most of the cancer deaths are caused by spread of the primary cancer to distant sites. Therefore, targeted anti-metastases therapies are needed to prevent cancer cells from metastasizing to different organs. The phage clone pc3-1 displaying peptide WSGPGVWGASVK selected by phage display had been identified which have high binding efficiency and remarkable cell specificity to SK-OV-3 cells. In the present work, the effects of selected cell-binding phage and cognate peptide on the cell adhesion and invasion of targeted cells were investigated. Results showed that the adhesive ability of SK-OV-3 to extracellular matrix was inhibited by pc3-1 and peptide WSGPGVWGASVK, and pc3-1 blocked SK-OV-3 cells attachment more effective than the cognate peptide. The peptide WSGPGVWGASVK suppressed the cell number of SK-OV-3 that attached to HUVECs monolayer up to 24% and could block the spreading of the attaching cells. Forthermore, the cognate peptide could inhibit the invasion of SK-OV-3 significantly. The number of invaded SK-OV-3 cells and invaded SK-OV-3-activated HUVECs pretreated with peptide WSGPGVWGASVK was decreased by 24.3% and 36.6%, respectively. All these results suggested that peptide WSGPGVWGASVK might possess anti-metastasis against SK-OV-3 cells.
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu 610065, PR China.
authors.forename: Ximing
authors.initials: X
authors.lastname: Pu
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu 610065, PR China.
authors.forename: Chuying
authors.initials: C
authors.lastname: Ma
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu 610065, PR China. Electronic address: nic0700@scu.edu.cn.
authors.forename: Guangfu
authors.initials: G
authors.lastname: Yin
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu 610065, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: You
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu 610065, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wei
mesh_terms: D000595:Amino Acid Sequence; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D061505:Cell Surface Display Techniques; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*
chemical_list: D010455:Peptides
keywords: Anti-metastasis; Cell adhesion; Cell invasion; Inhibitory effect; Ovarian cancer targeting peptide