title: Group-specific amplification of HLA-DQA1 revealed a number of genomic full-length sequences including the novel HLA alleles DQA1*01:10 and DQA1*01:11.
abstract: In this article, we describe a subgroup-specific amplification assay for HLA-DQA1 that encompasses the whole coding region and allows us to sequence full-length HLA-DQA1 genes. We introduce the novel alleles HLA-DQA1*01:10 and HLA-DQA1*01:11. Moreover, we were able to confirm the full-length genomic sequence data of the alleles HLA-DQA1*01:07, HLA-DQA1*03:01:01, HLA-DQA1*03:02, HLA-DQA1*04:01:02, HLA-DQA1*04:02, HLA-DQA1*05:03, HLA-DQA1*05:05:01:02 and HLA-DQA1*06:01:01. A complete genomic overview of all six HLA-DQA1 allele groups is now available from the submission of our data to the IMGT/HLA database. Because our approach facilitates the analysis of all HLA-DQA1 allele sequences, HLA-DQA1 may become the first HLA locus from which all subgroup members will be known in detail in the near future.
authors.affiliation: Laboratory of Immunogenetics, Department of Transfusion Medicine, Cell Therapeutic Agents and Hemostaseology, Ludwig-Maximilians University München, München, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Witter
authors.forename: J A
authors.initials: JA
authors.lastname: Halliwell
authors.forename: J
authors.initials: J
authors.lastname: Mautner
authors.forename: A
authors.initials: A
authors.lastname: Jolesch
authors.forename: G
authors.initials: G
authors.lastname: von Welser
authors.forename: I
authors.initials: I
authors.lastname: Rampp
authors.forename: M
authors.initials: M
authors.lastname: Spannagl
authors.forename: T
authors.initials: T
authors.lastname: Kauke
authors.forename: A
authors.initials: A
authors.lastname: Dick
mesh_terms: D000483:Alleles; D017931:DNA Primers / Q000235:genetics; D030561:Databases, Nucleic Acid; D016678:Genome; D005838:Genotype; D059848:HLA-DQ alpha-Chains / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D017931:DNA Primers; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen
keywords: HLA-DQA1; allele-specific amplification; complete genomic sequence; sequence-based typing