title: CD8(+)CD25(+) T cells reduce atherosclerosis in apoE(-/-) mice.
abstract: BACKGROUND
  It is increasingly evident that CD8(+) T cells are involved in atherosclerosis but the specific subtypes have yet to be defined. CD8(+)CD25(+) T cells exert suppressive effects on immune signaling and modulate experimental autoimmune disorders but their role in atherosclerosis remains to be determined. The phenotype and functional role of CD8(+)CD25(+) T cells in experimental atherosclerosis were investigated in this study.
  
  
  RESULTS
  CD8(+)CD25(+) T cells were observed in atherosclerotic plaques of apoE(-/-) mice fed hypercholesterolemic diet. Characterization by flow cytometric analysis and functional evaluation using a CFSE-based proliferation assays revealed a suppressive phenotype and function of splenic CD8(+)CD25(+) T cells from apoE(-/-) mice. Depletion of CD8(+)CD25(+) from total CD8(+) T cells rendered higher cytolytic activity of the remaining CD8(+)CD25(-) T cells. Adoptive transfer of CD8(+)CD25(+) T cells into apoE(-/-) mice suppressed the proliferation of splenic CD4(+) T cells and significantly reduced atherosclerosis in recipient mice.
  
  
  CONCLUSIONS
  Our study has identified an athero-protective role for CD8(+)CD25(+) T cells in experimental atherosclerosis.
authors.affiliation: Oppenheimer Atherosclerosis Research Center, Division of Cardiology, Cedars-Sinai Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, United States.
authors.forename: Jianchang
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Oppenheimer Atherosclerosis Research Center, Division of Cardiology, Cedars-Sinai Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, United States.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Dimayuga
authors.affiliation: Oppenheimer Atherosclerosis Research Center, Division of Cardiology, Cedars-Sinai Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, United States.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Oppenheimer Atherosclerosis Research Center, Division of Cardiology, Cedars-Sinai Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, United States.
authors.forename: Juliana
authors.initials: J
authors.lastname: Yano
authors.affiliation: Oppenheimer Atherosclerosis Research Center, Division of Cardiology, Cedars-Sinai Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, United States.
authors.forename: Wai Man
authors.initials: WM
authors.lastname: Lio
authors.affiliation: Oppenheimer Atherosclerosis Research Center, Division of Cardiology, Cedars-Sinai Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, United States.
authors.forename: Portia
authors.initials: P
authors.lastname: Trinidad
authors.affiliation: Oppenheimer Atherosclerosis Research Center, Division of Cardiology, Cedars-Sinai Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, United States.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Honjo
authors.affiliation: Oppenheimer Atherosclerosis Research Center, Division of Cardiology, Cedars-Sinai Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, United States.
authors.forename: Bojan
authors.initials: B
authors.lastname: Cercek
authors.affiliation: Oppenheimer Atherosclerosis Research Center, Division of Cardiology, Cedars-Sinai Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, United States.
authors.forename: Prediman K
authors.initials: PK
authors.lastname: Shah
authors.affiliation: Oppenheimer Atherosclerosis Research Center, Division of Cardiology, Cedars-Sinai Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, United States. Electronic address: Chyuk@cshs.org.
authors.forename: Kuang-Yuh
authors.initials: KY
authors.lastname: Chyu
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D001057:Apolipoproteins E / Q000172:deficiency* / Q000378:metabolism; D050197:Atherosclerosis / Q000097:blood / Q000276:immunology* / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation; D004032:Diet; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D058226:Plaque, Atherosclerotic / Q000097:blood / Q000276:immunology / Q000473:pathology; D013154:Spleen / Q000276:immunology / Q000473:pathology
chemical_list: D001057:Apolipoproteins E; D015415:Biomarkers; D053645:Interleukin-2 Receptor alpha Subunit
keywords: Adoptive transfer; Atherosclerosis; CD8(+)CD25(+) T cells; Immune modulation