title: RNase III controls mltD mRNA degradation in Escherichia coli.
abstract: RNase III is a double-stranded RNA-specific endoribonuclease that processes and degrades numerous mRNA molecules in Escherichia coli. A previous genome-wide analysis of E. coli transcripts showed that steady-state levels of mltD mRNA, which encodes membrane-bound lytic murein transglycosylase D, was most affected by changes in cellular concentration of RNase III. Consistent with this observation, in vitro and in vivo analyses of mltD mRNA revealed RNase III cleavage sites in the coding region of mltD mRNA. Introduction of a nucleotide substitution at the identified RNase III cleavage sites inhibited RNase III cleavage activity on mltD mRNA, resulting in, consequently, approximately two-fold increase in the steady-state level of the mRNA. These findings reveal an RNase III-mediated regulatory pathway that modulates mltD expression in E. coli.
authors.affiliation: Department of Life Science, Chung-Ang University, 84 Heuksok-Ro, Dongjak-Gu, Seoul, 156-756, Republic of Korea.
authors.forename: Boram
authors.initials: B
authors.lastname: Lim
authors.forename: Sangmi
authors.initials: S
authors.lastname: Ahn
authors.forename: Minji
authors.initials: M
authors.lastname: Sim
authors.forename: Kangseok
authors.initials: K
authors.lastname: Lee
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D020871:RNA Stability / Q000235:genetics / Q000502:physiology; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D016695:Glycosyltransferases; C021815:murein transglycosylase; D043244:Ribonuclease III