title: Interlaboratory comparison of size measurements on nanoparticles using nanoparticle tracking analysis (NTA).
abstract: One of the key challenges in the field of nanoparticle (NP) analysis is in producing reliable and reproducible characterisation data for nanomaterials. This study looks at the reproducibility using a relatively new, but rapidly adopted, technique, Nanoparticle Tracking Analysis (NTA) on a range of particle sizes and materials in several different media. It describes the protocol development and presents both the data and analysis of results obtained from 12 laboratories, mostly based in Europe, who are primarily QualityNano members. QualityNano is an EU FP7 funded Research Infrastructure that integrates 28 European analytical and experimental facilities in nanotechnology, medicine and natural sciences with the goal of developing and implementing best practice and quality in all aspects of nanosafety assessment. This study looks at both the development of the protocol and how this leads to highly reproducible results amongst participants. In this study, the parameter being measured is the modal particle size.
authors.affiliation: NanoSight Ltd., Minton Park, London Road, Amesbury, Wiltshire, SP4 7RT UK.
authors.forename: Patrick
authors.initials: P
authors.lastname: Hole
authors.affiliation: NanoSight Ltd., Minton Park, London Road, Amesbury, Wiltshire, SP4 7RT UK.
authors.forename: Katherine
authors.initials: K
authors.lastname: Sillence
authors.affiliation: NanoSight Ltd., Minton Park, London Road, Amesbury, Wiltshire, SP4 7RT UK.
authors.forename: Claire
authors.initials: C
authors.lastname: Hannell
authors.affiliation: Trinity Centre for Health Sciences, Department of Clinical Medicine, Institute of Molecular Medicine, St. James's Hospital, James Street, Dublin 8, Ireland.
authors.forename: Ciaran Manus
authors.initials: CM
authors.lastname: Maguire
authors.affiliation: Laboratory for Materials - Biology Interactions, Swiss Federal Laboratories for Materials Research and Testing (Empa), Lerchenfeldstrasse 5, 9014 St Gallen, Switzerland.
authors.forename: Matthias
authors.initials: M
authors.lastname: Roesslein
authors.affiliation: Institute for Work and Health, Rte. de la Corniche 2, 1066 Epalinges-Lausanne, Switzerland.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Suarez
authors.affiliation: NanoSight USA, 1415 Washington Heights, Rm 6611, Ann Arbor, MI 48109 USA.
authors.forename: Sonja
authors.initials: S
authors.lastname: Capracotta
authors.affiliation: Health Effects Laboratory, Department of Environmental Chemistry, Norwegian Institute for Air Research, Instituttveien 18, P.O. Box 100, NO-2027 Kjeller, Norway.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Magdolenova
authors.affiliation: Department of Physiology and Pharmacology, Sackler School of Medicine, Tel Aviv University, 69978 Tel Aviv, Israel.
authors.forename: Limor
authors.initials: L
authors.lastname: Horev-Azaria
authors.affiliation: Department of Earth Sciences, Natural History Museum, Cromwell Road, London, SW7 5BD UK.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Dybowska
authors.affiliation: Centre for Bio-Nano Interactions (CBNI), University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Laura
authors.initials: L
authors.lastname: Cooke
authors.affiliation: Experimental Research, Department of Product Safety, Bundesinstitut fur Risikobewertung (BfR), Max-Dohrn-Strasse 8-10, 10589 Berlin, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Haase
authors.affiliation: Département Environnement et Agro-biotechnologies (EVA), Centre de Recherche Public Gabriel Lippmann, 41, rue du Brill, 4422 Belvaux, Luxembourg.
authors.forename: Servane
authors.initials: S
authors.lastname: Contal
authors.affiliation: Health Effects Laboratory, Department of Environmental Chemistry, Norwegian Institute for Air Research, Instituttveien 18, P.O. Box 100, NO-2027 Kjeller, Norway.
authors.forename: Stein
authors.initials: S
authors.lastname: Manø
authors.affiliation: IBE R&D Institute for Lung Health gGmbH, Mendelstrasse 11, 48149 Münster, Germany.
authors.forename: Antje
authors.initials: A
authors.lastname: Vennemann
authors.affiliation: Institute for Work and Health, Rte. de la Corniche 2, 1066 Epalinges-Lausanne, Switzerland.
authors.forename: Jeans-Jacques
authors.initials: JJ
authors.lastname: Sauvain
authors.affiliation: Trinity Centre for Health Sciences, Department of Clinical Medicine, Institute of Molecular Medicine, St. James's Hospital, James Street, Dublin 8, Ireland.
authors.forename: Kieran Crosbie
authors.initials: KC
authors.lastname: Staunton
authors.affiliation: Centre for Bio-Nano Interactions (CBNI), University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Sergio
authors.initials: S
authors.lastname: Anguissola
authors.affiliation: Experimental Research, Department of Product Safety, Bundesinstitut fur Risikobewertung (BfR), Max-Dohrn-Strasse 8-10, 10589 Berlin, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Luch
authors.affiliation: Health Effects Laboratory, Department of Environmental Chemistry, Norwegian Institute for Air Research, Instituttveien 18, P.O. Box 100, NO-2027 Kjeller, Norway.
authors.forename: Maria
authors.initials: M
authors.lastname: Dusinska
authors.affiliation: Department of Physiology and Pharmacology, Sackler School of Medicine, Tel Aviv University, 69978 Tel Aviv, Israel.
authors.forename: Rafi
authors.initials: R
authors.lastname: Korenstein
authors.affiliation: Département Environnement et Agro-biotechnologies (EVA), Centre de Recherche Public Gabriel Lippmann, 41, rue du Brill, 4422 Belvaux, Luxembourg.
authors.forename: Arno C
authors.initials: AC
authors.lastname: Gutleb
authors.affiliation: IBE R&D Institute for Lung Health gGmbH, Mendelstrasse 11, 48149 Münster, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Wiemann
authors.affiliation: Trinity Centre for Health Sciences, Department of Clinical Medicine, Institute of Molecular Medicine, St. James's Hospital, James Street, Dublin 8, Ireland.
authors.forename: Adriele
authors.initials: A
authors.lastname: Prina-Mello
authors.affiliation: Institute for Work and Health, Rte. de la Corniche 2, 1066 Epalinges-Lausanne, Switzerland.
authors.forename: Michael
authors.initials: M
authors.lastname: Riediker
authors.affiliation: Laboratory for Materials - Biology Interactions, Swiss Federal Laboratories for Materials Research and Testing (Empa), Lerchenfeldstrasse 5, 9014 St Gallen, Switzerland.
authors.forename: Peter
authors.initials: P
authors.lastname: Wick
keywords: Health and safety implications; Interlaboratory comparison; Nanoparticle; Polydispersity; Reproducibility; Toxicology