title: Anti-inflammatory effects of 4-methylcyclopentadecanone on edema models in mice.
abstract: The present study evaluated the anti-inflammatory effects of 4-methylcyclopentadecanone (4-MCPC) on edema models in mice and aimed to determine the safety of 4-MCPC after acute exposure. The acute toxicity of 4-MCPC was evaluated by oral administration to rats of single doses of 0, 5, 50, 500 and 5000 mg/kg. Toxic symptoms were observed for 14 days. The anti-inflammatory activity was evaluated in xylene-induced mouse ear edema and carrageenan-induced mouse paw edema. The animals were treated with 4-MCPC once every day for seven consecutive days. Edema index, % inhibition, IL-1β, TNF-α, PGE2 and MPO levels in paws were detected after the treatment with xylene or carrageenan. Our results indicated that the LD50 value of 4-MCPC in rats is greater than 5000 mg/kg. The ED50 of 4-MCPC in xylene-induced mouse ear edema model was 7.5 mg/kg. 4-MCPC (8 or 16 mg/kg) remarkably inhibited carrageenan-induced mouse paw edema. Further study revealed that 4-MCPC treatment also decreased IL-1β, TNF-α, PGE2 and MPO levels in mice paws. Intragastric administration of 4-MCPC exhibited more significant anti-inflammatory activity than muscone at a dose of 16 mg/kg. Taken together, our results suggest that 4-MCPC has potent anti-inflammatory activity and the mechanisms might be related to the decreases of the levels of IL-1β, TNF-α, PGE2 and MPO in inflamed paws.
authors.forename: Yukui
authors.initials: Y
authors.lastname: Ma
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pharmacology, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China. yaoliduli@163.com.
authors.forename: Yingliang
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D002351:Carrageenan; D015232:Dinoprostone / Q000378:metabolism; D004195:Disease Models, Animal; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D053583:Interleukin-1beta / Q000378:metabolism; D007659:Ketones / Q000737:chemistry / Q000627:therapeutic use*; D047028:Macrocyclic Compounds / Q000737:chemistry / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014992:Xylenes
chemical_list: C000589480:4-methylcyclopentadecanone; D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D007659:Ketones; D047028:Macrocyclic Compounds; D014409:Tumor Necrosis Factor-alpha; D014992:Xylenes; D002351:Carrageenan; D009195:Peroxidase; D015232:Dinoprostone