title: The energy density of laser light differentially modulates the skin morphological reorganization in a murine model of healing by secondary intention.
abstract: This study investigates the influence of gallium-arsenide (GaAs) laser photobiostimulation applied with different energy densities on skin wound healing by secondary intention in rats. Three circular wounds, 10 mm in diameter, were made on the dorsolateral region of 21 Wistar rats weighting 282.12 ± 36.08 g. The animals were equally randomized into three groups: Group SAL, saline solution 0.9%; Group L3, laser GaAs 3 J/cm(2); Group L30, laser GaAs 30 J/cm(2). Analyses of cells, blood vessels, collagen and elastic fibres, glycosaminoglycans and wound contraction were performed on the scar tissue from different wounds every 7 days for 21 days. On day 7, 14 and 21, L3 and L30 showed higher collagen and glycosaminoglycan levels compared to SAL (P < 0.05). At day 21, elastic fibres were predominant in L3 and L30 compared to SAL (P < 0.05). Type-III collagen fibres were predominant at day 7 in both groups. There was gradual reduction in these fibres and accumulation of type-I collagen over time, especially in L3 and L30 compared with SAL. Elevated density of blood vessels was seen in L30 on days 7 and 14 compared to the other groups (P < 0.05). On these same days, there was higher tissue cellularity in L3 compared with SAL (P < 0.05). The progression of wound closure during all time points investigated was higher in the L30 group (P < 0.05). Both energy densities investigated increased the tissue cellularity, vascular density, collagen and elastic fibres, and glycosaminoglycan synthesis, with the greater benefits for wound closure being found at the density of 30 J/cm(2).
authors.affiliation: Department of General Biology, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil; Department of Biological Sciences and NUPEB, Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
authors.forename: Rômulo D
authors.initials: RD
authors.lastname: Novaes
authors.forename: Reggiani V
authors.initials: RV
authors.lastname: Gonçalves
authors.forename: Marli C
authors.initials: MC
authors.lastname: Cupertino
authors.forename: Bruna M
authors.initials: BM
authors.lastname: Araújo
authors.forename: Rafael M
authors.initials: RM
authors.lastname: Rezende
authors.forename: Eliziária C
authors.initials: EC
authors.lastname: Santos
authors.forename: João Paulo V
authors.initials: JP
authors.lastname: Leite
authors.forename: Sérgio Luis P
authors.initials: SL
authors.lastname: Matta
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000293:injuries* / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology*; D014947:Wounds and Injuries / Q000378:metabolism / Q000532:radiotherapy*
chemical_list: D003094:Collagen
keywords: collagen; laser photobiostimulation; morphology; pathology; skin repair; wound healing