title: Protective effect of the ultra-filtration extract from Xin Mai Jia on human aortic smooth muscle cell injury induced by hydrogen peroxide.
abstract: The aim of the present study was to explore whether an ultra-filtration extract from Xin Mai Jia (XMJ), a Chinese medicinal formulation, has a protective effect on human aortic smooth muscle cell (HASMC) injury models induced by hydrogen peroxide (H2O2), and to consider the mechanism and efficacy of the therapeutic action of XMJ on atherosclerosis. HASMCs were injured by H2O2and then exposed to various concentrations of XMJ. The morphological changes, growth, proliferation, migration and cytokine release of HASMCs were detected using 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT), an enzyme-linked immunosorbent assay and a scratch adhesion test. H2O2significantly promoted the proliferation of HASMCs. The ultra-filtration extract from XMJ was observed to significantly attenuate the morphological changes of injured HASMCs, reduce the expression levels of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), interleukin (IL)-1, IL-6 and nuclear factor (NF)-κB, and increase the expression levels of matrix metalloproteinase (MMP)-2 and tissue inhibitor of metalloproteinase (TIMP). XMJ has clear anti-inflammatory and antioxidant effects, and significantly inhibits the proliferation and migration of HASMCs.
authors.affiliation: Department of Nephrology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wan
authors.affiliation: School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Inspection, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors.forename: Ruili
authors.initials: R
authors.lastname: Sun
authors.affiliation: College of Pharmacy, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors.forename: Guopin
authors.initials: G
authors.lastname: Pan
authors.affiliation: College of Pharmacy, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: College of Pharmacy, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Modern Technology Education Center, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors.forename: Guangrui
authors.initials: G
authors.lastname: Wan
authors.affiliation: Department of Nephrology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
authors.forename: Zhang-Suo
authors.initials: ZS
authors.lastname: Liu
keywords: Xin Mai Jia; human aortic smooth muscle cell; inflammation; migration; proliferation