title: Chronic high glucose and pyruvate levels differentially affect mitochondrial bioenergetics and fuel-stimulated insulin secretion from clonal INS-1 832/13 cells.
abstract: Glucotoxicity in pancreatic β-cells is a well established pathogenetic process in type 2 diabetes. It has been suggested that metabolism-derived reactive oxygen species perturb the β-cell transcriptional machinery. Less is known about altered mitochondrial function in this condition. We used INS-1 832/13 cells cultured for 48 h in 2.8 mm glucose (low-G), 16.7 mm glucose (high-G), or 2.8 mm glucose plus 13.9 mm pyruvate (high-P) to identify metabolic perturbations. High-G cells showed decreased responsiveness, relative to low-G cells, with respect to mitochondrial membrane hyperpolarization, plasma membrane depolarization, and insulin secretion, when stimulated acutely with 16.7 mm glucose or 10 mm pyruvate. In contrast, high-P cells were functionally unimpaired, eliminating chronic provision of saturating mitochondrial substrate as a cause of glucotoxicity. Although cellular insulin content was depleted in high-G cells, relative to low-G and high-P cells, cellular functions were largely recovered following a further 24-h culture in low-G medium. After 2 h at 2.8 mm glucose, high-G cells did not retain increased levels of glycolytic or TCA cycle intermediates but nevertheless displayed increased glycolysis, increased respiration, and an increased mitochondrial proton leak relative to low-G and high-P cells. This notwithstanding, titration of low-G cells with low protonophore concentrations, monitoring respiration and insulin secretion in parallel, showed that the perturbed insulin secretion of high-G cells could not be accounted for by increased proton leak. The present study supports the idea that glucose-induced disturbances of stimulus-secretion coupling by extramitochondrial metabolism upstream of pyruvate, rather than exhaustion from metabolic overload, underlie glucotoxicity in insulin-producing cells.
authors.affiliation: From the Department of Clinical Sciences in Malmö, Unit of Molecular Metabolism, Lund University Diabetes Centre, CRC, 20502 Malmö, Sweden and.
authors.forename: Isabel
authors.initials: I
authors.lastname: Göhring
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Sharoyko
authors.forename: Siri
authors.initials: S
authors.lastname: Malmgren
authors.forename: Lotta E
authors.initials: LE
authors.lastname: Andersson
authors.forename: Peter
authors.initials: P
authors.lastname: Spégel
authors.forename: David G
authors.initials: DG
authors.lastname: Nicholls
authors.forename: Hindrik
authors.initials: H
authors.lastname: Mulder
mesh_terms: D002460:Cell Line; D002952:Citric Acid Cycle / Q000187:drug effects* / Q000502:physiology; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D006019:Glycolysis / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007328:Insulin; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000378:metabolism*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects* / Q000502:physiology; D019289:Pyruvic Acid / Q000378:metabolism / Q000494:pharmacology*; D013549:Sweetening Agents / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D007328:Insulin; D013549:Sweetening Agents; D019289:Pyruvic Acid; D005947:Glucose
keywords: Beta Cell; Bioenergetics; Insulin Secretion; Metabolomics; Mitochondrial Metabolism; Uncoupling Proteins