title: The siderophore-interacting protein is involved in iron acquisition and virulence of Riemerella anatipestifer strain CH3.
abstract: Riemerella anatipestifer causes epizootic infectious disease in poultry and serious economic losses especially to the duck industry. However, little is known regarding the molecular basis of its pathogenesis. The ability to acquire iron under low-iron conditions is related to the virulence of a variety of bacterial pathogens. In this study, a sip (Riean_1281) deletion mutant CH3Δsip was constructed and characterized for iron-limited growth, biofilm formation, and pathogenicity to ducklings. Results showed that siderophore-interacting protein (SIP) was involved in iron utilization and the sip deletion significantly reduced biofilm formation and adherence to and invasion of Vero cells. In addition, the sip gene was absent in 1 of 24 (4.17%) virulent strains and 2 of 3 (66.7%) avirulent strains of R. anatipestifer, and the sip gene from six R. anatipestifer strains, which belong to serotypes 1, 2, and 10, respectively, shared 100% amino acid identities to those of R. anatipestifer strains DSM15868 and RA-GD. These results suggested that siderophore-mediated iron acquisition may be an important iron-uptake pathway in R. anatipestifer. Animal experiments indicated that the median lethal dose of the CH3Δsip mutant in ducklings was about 35-fold higher than that of the wild-type CH3 strain. Thus, our results demonstrated that R. anatipestifer SIP was involved in iron acquisition and necessary for its optimal virulence.
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), 518 Ziyue Road, Shanghai 200241, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tu
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), 518 Ziyue Road, Shanghai 200241, China.
authors.forename: Fengying
authors.initials: F
authors.lastname: Lu
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), 518 Ziyue Road, Shanghai 200241, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Miao
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), 518 Ziyue Road, Shanghai 200241, China.
authors.forename: Xintao
authors.initials: X
authors.lastname: Ni
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), 518 Ziyue Road, Shanghai 200241, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), 518 Ziyue Road, Shanghai 200241, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yu
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), 518 Ziyue Road, Shanghai 200241, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Xing
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), 518 Ziyue Road, Shanghai 200241, China.
authors.forename: Shengqing
authors.initials: S
authors.lastname: Yu
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), 518 Ziyue Road, Shanghai 200241, China.
authors.forename: Chan
authors.initials: C
authors.lastname: Ding
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), 518 Ziyue Road, Shanghai 200241, China. Electronic address: huqinghai@caas.cn.
authors.forename: Qinghai
authors.initials: Q
authors.lastname: Hu
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002522:Chlorocebus aethiops; D004372:Ducks; D045826:Flavobacteriaceae Infections / Q000378:metabolism / Q000382:microbiology* / Q000662:veterinary; D017353:Gene Deletion; D007501:Iron / Q000378:metabolism*; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D011200:Poultry; D011201:Poultry Diseases / Q000378:metabolism / Q000382:microbiology*; D060494:Riemerella / Q000145:classification / Q000378:metabolism* / Q000472:pathogenicity; D017262:Siderophores / Q000235:genetics / Q000378:metabolism*; D014709:Vero Cells; D014774:Virulence / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D050505:Mutant Proteins; D017262:Siderophores; D007501:Iron
keywords: Deletion mutant; Iron acquisition; Riemerella anatipestifer; Siderophore-interacting protein (SIP); Virulence