title: Chemoenzymatic bio-orthogonal chemistry for site-specific double modification of recombinant thrombomodulin.
abstract: Best of both worlds: A one-pot strategy for site-specific PEGylation through strain-promoted alkyne-azide cycloaddition (SPAAC) and fluorescent labeling through sortase A-mediated ligation (SML) of recombinant thrombomodulin without prior chemical modification and without diminishing the protein activity has been developed.
authors.affiliation: Department of Chemistry, Chemical and Biomedical Engineering, Cleveland State University, 2121 Euclid Ave, Cleveland, OH 44115 (USA).
authors.forename: Rui
authors.initials: R
authors.lastname: Jiang
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Jacob
authors.initials: J
authors.lastname: Weingart
authors.forename: Xue-Long
authors.initials: XL
authors.lastname: Sun
mesh_terms: D000480:Alkynes / Q000737:chemistry; D019881:Aminoacyltransferases / Q000378:metabolism; D001386:Azides / Q000737:chemistry; D001426:Bacterial Proteins / Q000378:metabolism; D001896:Boron Compounds / Q000737:chemistry; D002384:Catalysis; D003300:Copper / Q000737:chemistry; D061565:Cycloaddition Reaction; D003546:Cysteine Endopeptidases / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D018180:Thrombomodulin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D000480:Alkynes; D001386:Azides; D001426:Bacterial Proteins; D001896:Boron Compounds; D005456:Fluorescent Dyes; D011994:Recombinant Proteins; D018180:Thrombomodulin; D011092:Polyethylene Glycols; D003300:Copper; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases
keywords: PEGylation; cycloadditions; fluorescent labeling; ligations; one-pot syntheses; thrombomodulin