title: [Genetic polymorphisms of 16 STR loci in Tibetan Mastiff].
abstract: OBJECTIVE
  To explore the genetic polymorphisms of 16 STR loci from 449 Tibetan Mastiffs in order to set up gene polymorphism database of Tibetan Mastiff.
  
  
  METHODS
  The PCR amplification was performed using the 16 STR loci fluorescent multiple amplification kit for dog. The amplified products were detected and statistically analyzed.
  
  
  RESULTS
  In the 16 STR loci from 449 Tibetan Mastiffs, CDP was 0.999 999 999 999 999 and CEP was 0.999 997 795. Except FH2010 (10 alleles), PEZ21 (12 alleles), and PEZ05 (13 alleles), the other STR loci had more than 15 alleles. In the 16 STR loci, H was > 0.5 and PIC was > 0.7.
  
  
  CONCLUSIONS
  The 16 STR loci have high polymorphism to be suitable for individual identification and paternity testing of Tibetan Mastiff. The data obtained through this study can be used to establish DNA polymorphism database of Tibetan Mastiff.
authors.affiliation: Center of Forensic Science, Beijing Genomics Institute, Beijing 101318, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Xiong
authors.affiliation: China Animal Agriculture Association National Kennel Club, Beijing 100028, China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Zhang
authors.affiliation: Center of Forensic Science, Beijing Genomics Institute, Beijing 101318, China.
authors.forename: Li-Wei
authors.initials: LW
authors.lastname: Mu
authors.affiliation: Center of Forensic Science, Beijing Genomics Institute, Beijing 101318, China.
authors.forename: Meng-Lei
authors.initials: ML
authors.lastname: Wang
authors.affiliation: Center of Forensic Science, Beijing Genomics Institute, Beijing 101318, China.
authors.forename: Jin-Long
authors.initials: JL
authors.lastname: Yang
authors.affiliation: Center of Forensic Science, Beijing Genomics Institute, Beijing 101318, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Center of Forensic Science, Beijing Genomics Institute, Beijing 101318, China.
authors.forename: Jiu-Wang
authors.initials: JW
authors.lastname: Mang
authors.affiliation: Center of Forensic Science, Beijing Genomics Institute, Beijing 101318, China.
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Deng
mesh_terms: D000483:Alleles; D000818:Animals; D004285:Dogs / Q000235:genetics*; D005787:Gene Frequency; D056426:Genetic Loci / Q000235:genetics; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic