title: Two copies of blaNDM-1 gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia.
abstract: New Delhi metallo-β-lactamase producing Pseudomonas aeruginosa isolates are of special interest since P. aeruginosa is a major cause of nosocomial infections, the treatment of which could now be jeopardized, especially in developing countries. Six additional NDM-1 positive P. aeruginosa clinical isolates belonging to two different genotypes were shown to be plasmid-free. PFGE-hybridization experiments revealed the chromosomal location of the blaNDM-1 gene. Restriction analysis and hybridization revealed that two copies of the blaNDM-1 gene are present in the genomes of all tested isolates, as in previously characterized P. aeruginosa MMA83. Moreover, it was shown that increasing imipenem concentration did not have the effect on copy number of the blaNDM-1 gene in the genome of P. aeruginosa MMA83.
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, P.O. Box 23, 11010, Belgrade, Serbia, bjovcic@bio.bg.ac.rs.
authors.forename: Branko
authors.initials: B
authors.lastname: Jovčić
authors.forename: Zorica
authors.initials: Z
authors.lastname: Lepšanović
authors.forename: Jelena
authors.initials: J
authors.lastname: Begović
authors.forename: Brankica
authors.initials: B
authors.lastname: Filipić
authors.forename: Milan
authors.initials: M
authors.lastname: Kojić
mesh_terms: D015373:Bacterial Typing Techniques; D016521:Electrophoresis, Gel, Pulsed-Field; D018628:Gene Dosage; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D055771:Serbia; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D001618:beta-Lactamases; C552864:beta-lactamase NDM-1