title: Glucoamylase is a major allergen of Schizophyllum commune.
abstract: BACKGROUND
  Schizophyllum commune is one of the causative agents of basidiomycosis including disorders such as allergic bronchopulmonary mycosis, allergic fungal sinusitis, and mucoid impaction of bronchi, the incidence of those of which has been increasing. These mycoses are difficult to diagnose because only a limited number of diagnostic tools are currently available. The biggest problem is that no specific antigens of S. commune have been identified to enable serodiagnosis of the disease.
  
  
  OBJECTIVE
  In this study, we attempted to identify a major antigen of S. commune to establish a reliable serodiagnostic method.
  
  
  METHODS
  We used mass spectrometry to identify an antigen that reacted with the serum of a patient with allergic bronchopulmonary mycosis caused by S. commune. The protein was expressed in Escherichia coli, highly purified, and the patient sera IgG and IgE titres against the protein were determined by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The protein identified as a major antigen of S. commune was named Sch c 1; it was a homolog of glucoamylase. The IgG and IgE titres against Sch c 1 in patient sera were significantly higher than those in healthy volunteer sera (P < 0.01).
  
  
  CONCLUSIONS
  Sch c 1 is recognized by the host immune system of patients as an antigen/allergen. The purified glucoamylase Sch c 1 is a promising candidate antigen for the serodiagnosis of S. commune-induced mycosis.
authors.affiliation: Division of Clinical Research, Medical Mycology Research Center, Chiba University, Chiba, Japan; Research Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Toyotome
authors.forename: M
authors.initials: M
authors.lastname: Satoh
authors.forename: M
authors.initials: M
authors.lastname: Yahiro
authors.forename: A
authors.initials: A
authors.lastname: Watanabe
authors.forename: F
authors.initials: F
authors.lastname: Nomura
authors.forename: K
authors.initials: K
authors.lastname: Kamei
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000276:immunology*; D000595:Amino Acid Sequence; D000908:Antibodies, Fungal / Q000097:blood / Q000276:immunology; D000946:Antigens, Fungal / Q000737:chemistry / Q000276:immunology*; D003429:Cross Reactions / Q000276:immunology; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008969:Molecular Sequence Data; D009181:Mycoses / Q000097:blood / Q000276:immunology*; D055732:Pulmonary Aspergillosis / Q000276:immunology; D012567:Schizophyllum / Q000201:enzymology / Q000276:immunology*; D016415:Sequence Alignment
chemical_list: D000485:Allergens; D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D007074:Immunoglobulin G; D007073:Immunoglobulin E; D005087:Glucan 1,4-alpha-Glucosidase
keywords: Sch c 1; Schizophyllum commune; allergic bronchopulmonary mycosis; basidiomycosis; glucoamylase