title: [Investigation of the venodilatory effect of vascular endothelial growth factor (VEGF) in rat gingiva].
abstract: VEGF induces proliferation of endothelial cells, stimulates angiogenesis, and increases vascular permeability in many organs. Nevertheless, we have only limited information about its role on gingival hemodynamics, especially in venules. Therefor the aim of this study was to assess the acute circulatory effects of VEGF on rat gingival venules by means of the following protocol. Wister rats (n=63) were devided into five study groups after anesthesia; each animal received 10 microl of experimental solution dripped onto the lower interincisal gingiva. The groups included: 1) saline control (after the experiment, gingiva was excised for VEGF receptor 2 [VEGFR2] immunohistochemistry); 2) VEGF (0.1, 1, 10, or 50 microg/ml); 3) VEGF2 receptor antagonist 5-((7-benzyloxyquinazolin-4-yl)amino)-4-fluoro-2-methyl-phenol-hydrochloride (ZM323881; 20 microg/ml); 4) ZM323881 (20 microg/ml) followed by VEGF application (50 microg/ml after 15 minutes); and 5) VEGF (10 microg/ml), these rats were premedicated with nitric oxide (NO) synthase blocker (NG-nitro-L-arginine-methyl-ester [L-NAME]; 1 mg/ml in drinking water) for 1 week before the experiment. Changes in gingival superficial venule diameter were measured by vital microscopy prior to and 1, 5, 15, 30, and 60 minutes after the administration of the experimental solutions. According to our findings, VEGF dose-dependently increased the venular diameter compared to saline. ZM323881 alone did not cause any alteration. Premedication with ZM323881 or L-NAME decreased the dilatory effects of VEGF. Occassionally moderate VEGFR2 immunohistochemical labeling was observed in the wall components of the venules. Concluding our results we can say, that there is no remarkable VEGF production under physiologic circumstances in rat gingiva, but VEGF is able to increase gingival blood flow through the activation of VEGF2 receptors and consequent NO release.
authors.affiliation: Semmelweis Egyetem, Fogorvostudományi Kar, Konzerváló Fogászati Klinika, Budapest.
authors.forename: Milán
authors.initials: M
authors.lastname: Gyurkovics
authors.affiliation: Semmelweis Egyetem, Fogorvostudományi Kar, Konzerváló Fogászati Klinika, Budapest.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Lohinai
authors.affiliation: Semmelweis Egyetem, Fogorvostudományi Kar, Konzerváló Fogászati Klinika, Budapest.
authors.forename: Adrienne
authors.initials: A
authors.lastname: Gyorfi
authors.affiliation: Semmelweis Egyetem, Altalános Orvostudományi Kar, Anatómiai Szōvet- es Fejlodéstani Intézet, Budapest.
authors.forename: D Andrea
authors.initials: DA
authors.lastname: Székely
authors.affiliation: Semmelweis Egyetem, Altalános Orvostudományi Kar, Egészségūgyi Informatikai Fejlesztési es Továbbképzo Intézet, Budapest.
authors.forename: Elek
authors.initials: E
authors.lastname: Dinya
authors.affiliation: Semmelweis Egyetem, Fogorvostudományi Kar, Konzerváló Fogászati Klinika, Budapest.
authors.forename: Arpád
authors.initials: A
authors.lastname: Fazekas
authors.affiliation: Semmelweis Egyetem, Altalános Orvostudományi Kar, Kórélettani Intézet, Magyar Tudományos Akadémia es Semmelweis Egyetem Gyermekgyógyászati es Nefrológiai Kutatócsoport, Budapest.
authors.forename: László
authors.initials: L
authors.lastname: Rosivall
mesh_terms: D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005881:Gingiva / Q000098:blood supply*; D007150:Immunohistochemistry; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D009389:Neovascularization, Pathologic; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D011799:Quinazolines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D040262:Receptors, Vascular Endothelial Growth Factor / Q000037:antagonists & inhibitors; D042461:Vascular Endothelial Growth Factor A / Q000187:drug effects / Q000378:metabolism*; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000187:drug effects / Q000378:metabolism*; D014664:Vasodilation / Q000187:drug effects*; D014699:Venules / Q000187:drug effects*
chemical_list: D004791:Enzyme Inhibitors; D011799:Quinazolines; D042461:Vascular Endothelial Growth Factor A; C478783:ZM323881; C467498:vascular endothelial growth factor A, rat; D019001:Nitric Oxide Synthase; D040262:Receptors, Vascular Endothelial Growth Factor; D040281:Vascular Endothelial Growth Factor Receptor-1; D019331:NG-Nitroarginine Methyl Ester