title: Colorimetric detection of DNA hybridization based on a dual platform of gold nanoparticles and graphene oxide.
abstract: The unique property of gold nanoparticles (Au NP) to induce colour change and the versatility of graphene oxides (GO) in surface modification makes them ideal in the application of colorimetric biosensor. Thus we developed a label free optical method to detect DNA hybridization through a visually observed colour change. The Au NP is conjugated to a DNA probe and is allowed to hybridize with the DNA target to the GO thus causing a change in colour from pinkish-red to purplish blue. Spectrophometry analysis gave a wavelength shift of 22 nm with 1 µM of DNA target. Sensitivity testing using serially diluted DNA conjugated GO showed that the optimum detection was at 63 nM of DNA target with the limit at 8 nM. This proves the possibility for the detection of DNA hybridization through the use of dual nanoparticle system by visual observation.
authors.affiliation: Low Dimension Material Research Center, Department of Physics, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: jeevanmacho@yahoo.com.
authors.forename: Jeevan
authors.initials: J
authors.lastname: Thavanathan
authors.affiliation: Low Dimension Material Research Center, Department of Physics, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: huangnayming@gmail.com.
authors.forename: Nay Ming
authors.initials: NM
authors.lastname: Huang
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: thongkl@um.edu.my.
authors.forename: Kwai Lin
authors.initials: KL
authors.lastname: Thong
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003124:Colorimetry / Q000295:instrumentation*; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D017403:In Situ Hybridization / Q000295:instrumentation*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000295:instrumentation
chemical_list: D010087:Oxides; D006046:Gold; D006108:Graphite; D004247:DNA
keywords: Colorimetric biosensor; Colorimetric detection; DNA hybridization; DNA probe; Gold nanoparticles; Graphene oxide