title: Metabolism, distribution, and excretion of deoxynivalenol with combined techniques of radiotracing, high-performance liquid chromatography ion trap time-of-flight mass spectrometry, and online radiometric detection.
abstract: Dispositions of deoxynivalenol (DON) in rats and chickens were investigated, using a radiotracer method coupled with a novel γ-accurate radioisotope counting (γ-ARC) radio-high-performance liquid chromatography ion trap time-of-flight tandem mass spectrometry (radio-HPLC-IT-TOF-MS/MS) system. 3β-(3)H-DON was chemically synthesized and orally administrated to both sexes of rats and chickens as single or multiple doses. The results showed that DON was widely distributed and quickly eliminated in all tissues. The highest concentration was found in the gastrointestinal tract at 6 h post-administration. Substantially lower levels were detected in the kidney, liver, heart, lung, spleen, and brain. Three new metabolites were identified tentatively as 10-deoxynivalenol-sulfonate, 10-deepoxy-deoxynivalenol (DOM-1)-sulfonate, and deoxynivalenol-3α-sulfate. Deoxynivalenol-3α-sulfate was a major metabolite in chickens, while the major forms in rats were DOM-1 and DON. Additionally, a higher excretion rate in urine was observed in female rats than in male rats. The differences in metabolite profiles and excretion rates, which suggested diverse ways to detoxify, may relate to the different tolerances in different genders or species.
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues (HZAU) and Ministry of Agriculture (MOA) Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University , Wuhan, Hubei 430070, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wan
authors.forename: Lingli
authors.initials: L
authors.lastname: Huang
authors.forename: Yuanhu
authors.initials: Y
authors.lastname: Pan
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Wu
authors.forename: Dongmei
authors.initials: D
authors.lastname: Chen
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Tao
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Zhenli
authors.initials: Z
authors.lastname: Liu
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Liye
authors.initials: L
authors.lastname: Wang
authors.forename: Zonghui
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D000818:Animals; D001331:Automation / Q000379:methods; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005243:Feces / Q000737:chemistry; D005260:Female; D007553:Isotope Labeling; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D055432:Metabolomics; D009183:Mycotoxins / Q000737:chemistry* / Q000378:metabolism / Q000652:urine; D011874:Radiometry / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D014255:Trichothecenes / Q000737:chemistry* / Q000378:metabolism / Q000652:urine; D014316:Tritium / Q000737:chemistry
chemical_list: D009183:Mycotoxins; D014255:Trichothecenes; D014316:Tritium; C007262:deoxynivalenol
keywords: deoxynivalenol; disposition; metabolism; tritium labeled