title: Impact of actin on adhesion and translocation of Enterococcus faecalis.
abstract: This study focuses on the impact of actin on adhesion and translocation of Enterococcus (E.) faecalis OG1RF, E. faecalis Symbioflor(®), and E. faecalis V583. Insight into the role of actin aggregation in the mediation of bacterial adhesion and translocation was provided by a two-chamber translocation assay, which employed Ptk6 cells. Determination of translocation rates, cytochalasin D treatment, and laser scanning confocal microscopic observation revealed actin as a predominant brace for enterococci to pass through the epithelial cell layer. As the three enterococci had moderate adhesion ability to actin, actin-binding proteins were isolated and characterized by LC-MS/MS. The isolated proteins were identified as pyruvate formate lyase, enolase, glyceraldehyde-3-phosphate dehydrogenase, and GroEL. All these proteins belong to two major groups of moonlighting proteins, i.e., proteins, which display additional functions other than their described major biochemical catalysis. Both groups of moonlight proteins were determined to be associated with epithelial cell binding.
authors.affiliation: Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Weihenstephaner Steig 16, 85350, Freising, Germany.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Peng
authors.forename: Viktoria
authors.initials: V
authors.lastname: Krey
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
authors.forename: Qianglai
authors.initials: Q
authors.lastname: Tan
authors.forename: Roger
authors.initials: R
authors.lastname: Vogelmann
authors.forename: Matthias A
authors.initials: MA
authors.lastname: Ehrmann
authors.forename: Rudi F
authors.initials: RF
authors.lastname: Vogel
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism; D002460:Cell Line; D013293:Enterococcus faecalis / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D051379:Mice; D008840:Microfilament Proteins / Q000302:isolation & purification / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D000199:Actins; D001426:Bacterial Proteins; D008840:Microfilament Proteins