title: Blood methylomics in response to arsenic exposure in a low-exposed US population.
abstract: Exposure to arsenic (As) has been associated with a number of diseases such as cancers, cardiovascular diseases (CVD), and neurological disorders. To explore the possible underlying epigenetic mechanisms, a nested case-control study was conducted within the Coronary Artery Risk Development in Young Adults (CARDIA) study by randomly selecting 46 non-smoker and non-diabetic White participants with low (N=23) and high (N=23) As exposure based on toenail total As measures at examination year 2. We conducted methylomic profiling of white blood cell (WBC) DNA collected at examination year 15 using the Illumina HumanMethylation450 BeadChip, and performed association tests using multiple linear regression models adjusting for age, sex, and estimated WBC proportions. We observed 22 CpG sites with methylation levels associated with high As exposure at a nominal significance level of 10(-4). However, the statistical significance disappeared after correction for multiple testing. Some genes annotated by these 22 CpG sites are known to be involved in As-associated diseases. Replication in larger samples of individuals with low levels of As exposure will be required.
authors.affiliation: 1] Mary Ann and J. Milburn Smith Child Health Research Program, Ann and Robert H. Lurie Children's Hospital of Chicago Research Center, Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA [2] Department of Preventive Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Driskill Graduate Program (DGP) in Life Sciences, Northwestern University Biomedical Informatics Center (NUBIC), Northwestern University Clinical and Translational Sciences Institute (NUCATS), Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.
authors.forename: Yinan
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Pediatrics, Institute of Human Genetics, University of Illinois, Chicago, Illinois, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Preventive Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Preventive Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.
authors.forename: Donald M
authors.initials: DM
authors.lastname: Lioyd-Jones
authors.affiliation: Department of Environmental Health, Harvard School of Public Health, Boston, Massachusetts, USA.
authors.forename: Andrea A
authors.initials: AA
authors.lastname: Baccarelli
authors.affiliation: Department of Preventive Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Ning
authors.affiliation: Institute of Molecular Medicine and School of Public Health, Division of Epidemiology Human Genetics and Environmental Sciences, University of Texas Health Sciences Center at Houston, Houston, Texas, USA.
authors.forename: Myriam
authors.initials: M
authors.lastname: Fornage
authors.affiliation: Department of Epidemiology and Biostatistics, Indiana University School of Public Health, Bloomington, Indiana, USA.
authors.forename: Ka
authors.initials: K
authors.lastname: He
authors.affiliation: Department of Preventive Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.
authors.forename: Kiang
authors.initials: K
authors.lastname: Liu
authors.affiliation: 1] Department of Preventive Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA [2] The Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.
authors.forename: Lifang
authors.initials: L
authors.lastname: Hou
mesh_terms: D001151:Arsenic / Q000032:analysis / Q000633:toxicity*; D016022:Case-Control Studies; D018899:CpG Islands; D019175:DNA Methylation; D004781:Environmental Exposure; D006801:Humans; D011446:Prospective Studies; D014481:United States
chemical_list: D001151:Arsenic