title: Vitamin B12-loaded solid lipid nanoparticles as a drug carrier in cancer therapy.
abstract: Nanostructure-mediated drug delivery, a key technology for the realization of nanomedicine, has the potential to improve drug bioavailability, ameliorate release deviation of drug molecules and enable precision drug targeting. Due to their multifunctional properties, solid lipid nanoparticles (SLNs) have received great attention of scientists to find a solution to cancer. Vitamin supplements may contribute to a reduction in the risk of cancer. Vitamin B12 has several characteristics that make it an attractive entity for cancer treatment and possible therapeutic applications. The aim of this study was to produce B12-loaded SLNs (B12-SLNs) and determine the cytotoxic effects of B12-SLNs on H-Ras 5RP7 and NIH/3T3 control cell line. Results obtained by MTT assay, transmission electron and confocal microscopy showed that B12-loaded SLNs are more effective than free vitamin B12 on cancer cells. In addition, characterization studies indicate that while the average diameter of the B12 was about 650 nm, B12-SLNs were about 200 nm and the drug release efficiency of vit. B12 by means of SLNs increased up to 3 h. These observations point to the fact that B12-SLNs could be used as carrier systems due to the therapeutic effects on cancer.
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmacy .
authors.forename: Lütfi
authors.initials: L
authors.lastname: Genç
authors.forename: H Mehtap
authors.initials: HM
authors.lastname: Kutlu
authors.forename: Gamze
authors.initials: G
authors.lastname: Güney
mesh_terms: D000818:Animals; D002461:Cell Line, Transformed; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D065546:Drug Liberation; D008055:Lipids / Q000737:chemistry; D051379:Mice; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D041681:NIH 3T3 Cells; D053758:Nanoparticles; D010316:Particle Size; D051381:Rats; D013997:Time Factors; D014805:Vitamin B 12 / Q000008:administration & dosage* / Q000494:pharmacology; D014803:Vitamin B Complex / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D004337:Drug Carriers; D008055:Lipids; D014803:Vitamin B Complex; D014805:Vitamin B 12
keywords: Cancer; drug delivery systems; solid lipid nanoparticles; vitamin B12