title: Role of Dectin-2 for host defense against systemic infection with Candida glabrata.
abstract: Although Candida glabrata is an important pathogenic Candida species, relatively little is known about its innate immune recognition. Here, we explore the potential role of Dectin-2 for host defense against C. glabrata. Dectin-2-deficient (Dectin-2(-/-)) mice were found to be more susceptible to C. glabrata infections, showing a defective fungal clearance in kidneys but not in the liver. The increased susceptibility to infection was accompanied by lower production of T helper 1 (Th1) and Th17-derived cytokines by splenocytes of Dectin-2(-/-) mice, while macrophage-derived cytokines were less affected. These defects were associated with a moderate yet significant decrease in phagocytosis of the fungus by the Dectin-2(-/-) macrophages and neutrophils. Neutrophils of Dectin-2(-/-) mice also displayed lower production of reactive oxygen species (ROS) upon challenge with opsonized C. glabrata or C. albicans. This study suggests that Dectin-2 is important in host defense against C. glabrata and provides new insights into the host defense mechanisms against this important fungal pathogen.
authors.affiliation: Radboud University Medical Center, Department of Internal Medicine, Division of Experimental Internal Medicine, Nijmegen, The Netherlands.
authors.forename: Daniela C
authors.initials: DC
authors.lastname: Ifrim
authors.forename: Judith M
authors.initials: JM
authors.lastname: Bain
authors.forename: Delyth M
authors.initials: DM
authors.lastname: Reid
authors.forename: Marije
authors.initials: M
authors.lastname: Oosting
authors.forename: Ineke
authors.initials: I
authors.lastname: Verschueren
authors.forename: Neil A R
authors.initials: NA
authors.lastname: Gow
authors.forename: J Han
authors.initials: JH
authors.lastname: van Krieken
authors.forename: Gordon D
authors.initials: GD
authors.lastname: Brown
authors.forename: Bart-Jan
authors.initials: BJ
authors.lastname: Kullberg
authors.forename: Leo A B
authors.initials: LA
authors.lastname: Joosten
authors.forename: Jos W M
authors.initials: JW
authors.lastname: van der Meer
authors.forename: Frank
authors.initials: F
authors.lastname: Koentgen
authors.forename: Lars P
authors.initials: LP
authors.lastname: Erwig
authors.forename: Jessica
authors.initials: J
authors.lastname: Quintin
authors.forename: Mihai G
authors.initials: MG
authors.lastname: Netea
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000276:immunology; D041221:Candida glabrata / Q000276:immunology*; D002177:Candidiasis / Q000276:immunology* / Q000382:microbiology; D016207:Cytokines / Q000276:immunology; D005260:Female; D037181:Lectins, C-Type / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000276:immunology / Q000382:microbiology; D010587:Phagocytosis / Q000276:immunology; D017382:Reactive Oxygen Species / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology / Q000382:microbiology
chemical_list: D016207:Cytokines; D037181:Lectins, C-Type; D017382:Reactive Oxygen Species; C445340:dectin-2, mouse