title: Microfluidic generation of polydopamine gradients on hydrophobic surfaces.
abstract: Engineered surface-bound molecular gradients are of great importance for a range of biological applications. In this paper, we fabricated a polydopamine gradient on a hydrophobic surface. A microfluidic device was used to generate a covalently conjugated gradient of polydopamine (PDA), which changed the wettabilty and the surface energy of the substrate. The gradient was subsequently used to enable the spatial deposition of adhesive proteins on the surface. When seeded with human adipose mesenchymal stem cells, the PDA-graded surface induced a gradient of cell adhesion and spreading. The PDA gradient developed in this study is a promising tool for controlling cellular behavior and may be useful in various biological applications.
authors.affiliation: WPI-Advanced Institute for Materials Research, Tohoku University , Sendai 980-8578, Japan.
authors.forename: Xuetao
authors.initials: X
authors.lastname: Shi
authors.forename: Serge
authors.initials: S
authors.lastname: Ostrovidov
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Shu
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Liang
authors.forename: Ken
authors.initials: K
authors.lastname: Nakajima
authors.forename: Hongkai
authors.initials: H
authors.lastname: Wu
authors.forename: Ali
authors.initials: A
authors.lastname: Khademhosseini
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D002448:Cell Adhesion; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007211:Indoles / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D007211:Indoles; D011108:Polymers; C568283:polydopamine