title: Inhibitory effect of putranjivain A on allergic inflammation through suppression of mast cell activation.
abstract: A great number of people are suffering from allergic inflammatory disease such as asthma, atopic dermatitis, and sinusitis. Therefore discovery of drugs for the treatment of these diseases is an important subject in human health. Putranjivain A (PJA), member of ellagitannin, is known to possess beneficial effects including anti-cancer and anti-viral activities. The aim of the present study was to elucidate whether PJA modulates the allergic inflammatory reaction and to study its possible mechanisms of action using mast cell-based in vitro and in vivo models. The study was performed in anaphylaxis mouse model and cultured mast cells. PJA inhibited the expression of pro-inflammatory cytokines in immunoglobulin E-stimulated mast cells. PJA reduced this expression by inhibiting nuclear factor (NF)-κB and nuclear factor of activated T cell. The oral administration of PJA reduced systemic and cutaneous anaphylaxis, the release of serum histamine, and the expression of the histamine H1 receptor. In addition, PJA attenuated the activation of mast cells. PJA inhibited the release of histamine from various types of mast cells by the suppression of intracellular calcium. The inhibitory activity of PJA on the allergic reaction was similar to that of disodium cromoglycate, a known anti-allergic drug. These results suggest that PJA can facilitate the prevention or treatment of allergic inflammatory diseases mediated by mast cells.
authors.affiliation: CMRI, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea.
authors.forename: Hui-Hun
authors.initials: HH
authors.lastname: Kim
authors.affiliation: CMRI, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea.
authors.forename: Seung-Bin
authors.initials: SB
authors.lastname: Park
authors.affiliation: CMRI, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea.
authors.forename: Soyoung
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Immunology, School of Medicine, Keimyung University, Daegu 704-701, Republic of Korea.
authors.forename: Taeg Kyu
authors.initials: TK
authors.lastname: Kwon
authors.affiliation: College of Pharmacy, Woosuk University, Jeonju 565-701, Republic of Korea.
authors.forename: Tae-Yong
authors.initials: TY
authors.lastname: Shin
authors.affiliation: College of Pharmacy, Youngnam University, Kyungsan 712-749, Republic of Korea.
authors.forename: Pil-Hoon
authors.initials: PH
authors.lastname: Park
authors.affiliation: College of Pharmacy, Youngnam University, Kyungsan 712-749, Republic of Korea.
authors.forename: Seung-Ho
authors.initials: SH
authors.lastname: Lee
authors.affiliation: CMRI, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea. Electronic address: shkim72@knu.ac.kr.
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000284:Administration, Oral; D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology; D002478:Cells, Cultured; D004205:Cromolyn Sodium / Q000494:pharmacology; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000378:metabolism; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D005960:Glucosides / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D006967:Hypersensitivity / Q000188:drug therapy; D007073:Immunoglobulin E / Q000378:metabolism; D007249:Inflammation / Q000188:drug therapy / Q000517:prevention & control*; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D011969:Receptors, Histamine H1 / Q000235:genetics / Q000378:metabolism
chemical_list: D018927:Anti-Asthmatic Agents; D016207:Cytokines; D005960:Glucosides; D016328:NF-kappa B; D011969:Receptors, Histamine H1; C479122:putranjivain A; D007073:Immunoglobulin E; D005707:Gallic Acid; D004205:Cromolyn Sodium
keywords: Allergic inflammation; DNP; HMC; Histamine; Mast cells; NF-κB; NFAT; PCA; PMA; Putranjivain A; RBL; dinitrophenyl; human mast cell; nuclear factor of activated T cell; passive cutaneous anaphylaxis; phorbol 12-myristate 13-acetate; rat basophilic leukemia