title: A BOX-SCAR fragment for the identification of Actinobacillus pleuropneumoniae.
abstract: Bacterial respiratory diseases are responsible for considerable mortality, morbidity and economic losses in the swine industry. Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia, is one of the most important disease agents, but its identification and surveillance can be impaired by the existence of many other related bacteria in normal swine microbiota. In this work, we have evaluated a BOX-A1R-based repetitive extragenic palindromic-PCR (BOX-PCR) sequence characterised amplified region (SCAR) marker for the specific identification of A. pleuropneumoniae and its use in a multiplex PCR to detect additionally Haemophilus parasuis and Pasteurella multocida, two other major respiratory pathogens of pigs that are members of the family Pasteurellaceae. PCRs based on the BOX-SCAR fragment developed were rapid, sensitive and differentiated A. pleuropneumoniae from all swine-related members of the Pasteurellaceae family tested. Single and multiplex BOX-SCAR fragment-based PCRs can be used to identify A. pleuropneumoniae from other bacterial swine pathogens and will be useful in surveillance and epidemiological studies.
authors.affiliation: Laboratório de Genética Molecular de Micro-organismos, Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária - BIOAGRO, Universidade Federal de Viçosa, Viçosa, Brazil.
authors.forename: Ciro C
authors.initials: CC
authors.lastname: Rossi
authors.forename: Monalessa F
authors.initials: MF
authors.lastname: Pereira
authors.forename: Paul R
authors.initials: PR
authors.lastname: Langford
authors.forename: Denise M S
authors.initials: DM
authors.lastname: Bazzolli
mesh_terms: D000189:Actinobacillus Infections / Q000382:microbiology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D000818:Animals; D055029:Inverted Repeat Sequences; D016133:Polymerase Chain Reaction / Q000379:methods*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
keywords: BOX-PCR; Pasteurellaceae; multiplex PCR; sequence characterised amplified region; swine pleuropneumonia