title: [Regulating effects of Rosa roxburghii tratt preparation on immune function in arseniasis patients caused by coal burning].
abstract: OBJECTIVE
  To explore the influence of rosa roxburghii tratt preparation on immune function of arseniasis patients caused by burning coal.
  
  
  METHODS
  According to the diagnosis standard for endemic arseniasis(WS/T 211-2001), 62 cases of arseniasis patients who resided in endemic arseniasis area in Guizhou province were selected and divided stratified randomly into two groups: rosa roxburghii tratt juice treatment group and superoxide dismutase(SOD)-enriched rosa roxburghii tratt juice treatment group, with 31 patients in each group.Each patient took 120 ml/d rosa roxburghii tratt preparation or SOD-enriched rosa roxburghii tratt orally for one month. Another 30 healthy residents from a neighbour township 12 km away where arsenic was not prevalent were selected as controls. A 2 ml blood and 50 ml urine samples were collected from individuals and the urine arsenic contents, peripheral blood T-lymphocyte subsets (CD3(+), CD4(+), CD8(+) T cell), serum immunoglobulin (IgG, IgM, IgA) and complement (C3, C4) were detected. The differences between more than two groups on above indicators were compared. The correlations between urinary arsenic and immune parameters were analyzed.
  
  
  RESULTS
  Among the rosa roxburghii tratt juice group, SOD-enriched rosa roxburghii tratt juice before intervention group and the control group, the levels of urine arsenic were (76.55 ± 23.02) , (72.60 ± 25.91) and (26.33 ± 11.30) µg/g Cr respectively and IgG were (11.31 ± 1.68), (11.35 ± 1.94) and (9.23 ± 1.75) g/L respectively. The differences were statistically significant(F values were 82.01, 13.82, both P values < 0.05). After intervention with rosa roxburghii tratt preparation, the levels of urine arsenic were (53.21 ± 16.51) and (51.72 ± 17.70)µg/g Cr, both decreased than before intervention (t values were 5.80 and 3.78, both P values < 0.05). The levels of CD3(+) were (44.47 ± 7.14)%, (43.44 ± 6.61)% and (70.78 ± 5.26)%, CD4(+) were (29.87 ± 5.67)%, (29.42 ± 5.87)% and (46.08 ± 5.87)%, CD4(+)/CD8(+) were(1.25 ± 0.42), (1.22 ± 0.39) and (1.79 ± 0.26) and C4 were (0.13 ± 0.08), (0.13 ± 0.09) and (0.20 ± 0.11) g/L respectively among the two treatment group before intervention and the control group. The differences were significant (F values were 178.04, 76.71, 23.13 and 5.26, all P values < 0.05). After intervention, the levels of CD3(+) were (59.73 ± 7.38)% and (66.31 ± 7.57)%, CD4(+) were (34.00 ± 7.97)% and (39.11 ± 5.81)%, CD4(+)/CD8(+) were (1.41 ± 0.37) and(1.58 ± 0.26), all increased than before intervention(t values were 12.47, 25.18, 5.41, 10.47, 3.22 and 5.05, all P values < 0.05). The levels of urine arsenic and CD3(+), CD4(+), CD4(+)/CD8(+), C4 were inversely correlated correlation, while positive correlation existed between the level of urine arsenic and IgG(r values were -0.68, -0.56, -0.51, -0.43 and 0.36, all P values < 0.01).
  
  
  CONCLUSIONS
  The level of urinary arsenic level is closely related to immune function suppression in arseniasis patients caused by burning coal, rosa roxburghii tratt preparation can effectively improve immune function of arseniasis patients.
authors.affiliation: School of Public Health, Guiyang Medical College, Guiyang 550004, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Email: 940879123@qq.com.
authors.forename: Ai-hua
authors.initials: AH
authors.lastname: Zhang
authors.forename: Yu-jiang
authors.initials: YJ
authors.lastname: Ren
authors.forename: Zhong-yi
authors.initials: ZY
authors.lastname: Liu
authors.forename: Xiao-xin
authors.initials: XX
authors.lastname: Huang
authors.forename: Da-ping
authors.initials: DP
authors.lastname: Yang
mesh_terms: D000328:Adult; D001151:Arsenic / Q000652:urine; D020261:Arsenic Poisoning / Q000209:etiology / Q000276:immunology*; D002681:China; D003031:Coal; D003165:Complement System Proteins / Q000276:immunology; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010936:Plant Extracts / Q000494:pharmacology*; D027826:Rosa / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000494:pharmacology*; D016176:T-Lymphocyte Subsets / Q000276:immunology
chemical_list: D003031:Coal; D007136:Immunoglobulins; D010936:Plant Extracts; D003165:Complement System Proteins; D013482:Superoxide Dismutase; D001151:Arsenic