title: Nanosecond ratio imaging of redox states in tumor cell spheroids using light sheet-based fluorescence microscopy.
abstract: A new concept of three-dimensional imaging of tumor cell spheroids by light sheet-based fluorescence microscopy and nanosecond ratio imaging is described. Due to its low light dose and alternative excitation by two laser wavelengths (391 and 470 nm), this method maintains cell viability and permits recording of real-time kinetics. A genetically encoded sensor permits measurement of the redox state of glutathione and visualization of the impact of oxygen radicals. The pharmaceutically relevant system is tested upon addition of an oxidizing agent (H2O2), as well as upon addition of the apoptosis-inducing agent staurosporine.
authors.affiliation: Hochschule Aalen, Institut für Angewandte Forschung, Beethovenstr. 1, 73430 Aalen, Germany.
authors.forename: Sarah
authors.initials: S
authors.lastname: Schickinger
authors.affiliation: Hochschule Aalen, Institut für Angewandte Forschung, Beethovenstr. 1, 73430 Aalen, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Bruns
authors.affiliation: Institut für Lasertechnologien in der Medizin und Messtechnik an der Universität Ulm, Helmholtzstr. 12, 89081 Ulm, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Wittig
authors.affiliation: Hochschule Aalen, Institut für Angewandte Forschung, Beethovenstr. 1, 73430 Aalen, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Weber
authors.affiliation: Hochschule Aalen, Institut für Angewandte Forschung, Beethovenstr. 1, 73430 Aalen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Wagner
authors.affiliation: Hochschule Aalen, Institut für Angewandte Forschung, Beethovenstr. 1, 73430 Aalen, GermanybInstitut für Lasertechnologien in der Medizin und Messtechnik an der Universität Ulm, Helmholtzstr. 12, 89081 Ulm, Germany.
authors.forename: Herbert
authors.initials: H
authors.lastname: Schneckenburger
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D045744:Cell Line, Tumor; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008856:Microscopy, Fluorescence / Q000379:methods*; D010084:Oxidation-Reduction / Q000187:drug effects; D018874:Spheroids, Cellular / Q000737:chemistry / Q000166:cytology; D019311:Staurosporine / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000166:cytology
chemical_list: D006861:Hydrogen Peroxide; D019311:Staurosporine