title: Mineralocorticoid receptor and heat shock protein expression levels in peripheral lymphocytes from war trauma-exposed men with and without PTSD.
abstract: Alterations in the number and functional status of mineralocorticoid (MR) and glucocorticoid receptors (GR) may contribute to vulnerability to posttraumatic stress disorder (PTSD). Corticosteroid receptors are chaperoned by heat shock proteins Hsp90 and Hsp70. We examined relations between corticosteroid receptor and heat shock protein expression levels, and related them with war trauma exposure, PTSD and resilience to PTSD. Relative levels of MR, Hsp90 and Hsp70 were determined by immunoblotting in lymphocytes from war trauma-exposed men with current PTSD (current PTSD group, n=113), with life-time PTSD (life-time PTSD group, n=61) and without PTSD (trauma control group, n=88), and from non-traumatized healthy controls (healthy control group, n=85). Between-group differences in MR, Hsp90 and Hsp70 levels and in MR/GR ratio were not observed. The level of MR was correlated with both Hsp90 and Hsp70 levels in trauma control and healthy control groups. On the other hand, GR level was correlated only with Hsp90 level, and this correlation was evident in current PTSD and trauma control groups. In conclusion, PTSD and exposure to trauma are not related to changes in lymphocyte MR, Hsp90 or Hsp70 levels, but may be associated with disturbances in corticosteroid receptors interaction with heat shock proteins.
authors.affiliation: University of Belgrade, Institute for Biological Research "Siniša Stanković", Department of Biochemistry, Belgrade, Serbia. Electronic address: gormatic@ibiss.bg.ac.rs.
authors.forename: Gordana
authors.initials: G
authors.lastname: Matić
authors.affiliation: University of Belgrade, Institute for Biological Research "Siniša Stanković", Department of Biochemistry, Belgrade, Serbia.
authors.forename: Danijela
authors.initials: D
authors.lastname: Vojnović Milutinović
authors.affiliation: University of Belgrade, Institute for Biological Research "Siniša Stanković", Department of Biochemistry, Belgrade, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Nestorov
authors.affiliation: University of Belgrade, Institute for Biological Research "Siniša Stanković", Department of Biochemistry, Belgrade, Serbia.
authors.forename: Ivana
authors.initials: I
authors.lastname: Elaković
authors.affiliation: University of Belgrade, Institute for Biological Research "Siniša Stanković", Department of Biochemistry, Belgrade, Serbia.
authors.forename: Sanja
authors.initials: S
authors.lastname: Manitašević Jovanović
authors.affiliation: University of Belgrade, Institute for Biological Research "Siniša Stanković", Department of Biochemistry, Belgrade, Serbia.
authors.forename: Younis Mouftah
authors.initials: YM
authors.lastname: Elzaedi
authors.affiliation: University of Belgrade, Institute for Biological Research "Siniša Stanković", Department of Biochemistry, Belgrade, Serbia.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Perišić
authors.affiliation: University of Belgrade, Institute for Biological Research "Siniša Stanković", Department of Biochemistry, Belgrade, Serbia.
authors.forename: Jadranka
authors.initials: J
authors.lastname: Dunđerski
authors.affiliation: University of Belgrade, School of Medicine, Clinical Center of Serbia, Institute of Endocrinology, Diabetes and Metabolic Diseases, Belgrade, Serbia.
authors.forename: Svetozar
authors.initials: S
authors.lastname: Damjanović
authors.affiliation: International Aid Network, Belgrade, Serbia; University of Belgrade, Faculty of Philosophy, Department of Psychology, Belgrade, Serbia.
authors.forename: Goran
authors.initials: G
authors.lastname: Knežević
authors.affiliation: International Aid Network, Belgrade, Serbia; Military Medical Academy, Belgrade, Serbia.
authors.forename: Željko
authors.initials: Ž
authors.lastname: Špirić
authors.affiliation: Military Mental Health-Research Center, University Medical Center, Rudolf Magnus Institute of Neurosciences, Utrecht, Netherlands.
authors.forename: Eric
authors.initials: E
authors.lastname: Vermetten
authors.affiliation: International Aid Network, Belgrade, Serbia; University of Belgrade, Vinča Institute of Nuclear Sciences, Laboratory for Theoretical and Condensed Matter Physics, Belgrade, Serbia.
authors.forename: Danka
authors.initials: D
authors.lastname: Savić
mesh_terms: D000328:Adult; D003130:Combat Disorders / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018161:Receptors, Mineralocorticoid / Q000378:metabolism*; D055771:Serbia; D013313:Stress Disorders, Post-Traumatic / Q000378:metabolism*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D018161:Receptors, Mineralocorticoid
keywords: Glucocorticoid receptor; Hsp70; Hsp90; Mineralocorticoid receptor; PTSD; War trauma