title: Plasma-derived mannose-binding lectin shows a direct interaction with C1-inhibitor.
abstract: MBL-deficiency has been associated with an increased frequency and severity of infection, in particular in children and under immunocompromized conditions. In an open uncontrolled safety and pharmacokinetic MBL-substitution study using plasma-derived MBL (pdMBL) in MBL-deficient pediatric oncology patients, we found that despite MBL trough levels above 1.0μg/ml MBL functionality was not efficiently restored upon ex vivo testing. PdMBL showed C4-converting activity by itself, indicating the presence of MASPs. Upon incubation of pdMBL with MBL-deficient sera this C4-converting activity was significantly reduced. Depletion of the MASPs from pdMBL, paradoxically, restored the C4-converting activity. Subsequent depletion or inhibition of C1-inh, the major inhibitor of the lectin pathway, in the recipient serum restored the C4-converting activity as well. Complexes between MBL/MASPs and C1-inh (MMC-complexes) were detected after ex vivo substitution of MBL-deficient serum with pdMBL. These MMC-complexes could also be detected in the sera of the patients included in the MBL-substitution study shortly after pdMBL infusion. Altogether, we concluded that active MBL-MASP complexes in pdMBL directly interact with C1-inh in the recipient, leading to the formation of a multimolecular complex between C1-inh and MBL/MASPs, in contrast to the classical pathway where C1r and C1s are dissociated from C1q by C1-inh. Because of the presence of activated MASPs in the current pdMBL products efficient MBL-mediated host protection cannot be expected because of the neutralizing capacity by C1-inh.
authors.affiliation: Emma Children's Hospital, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands; Department of Immunopathology, Sanquin Research and Landsteiner Laboratory, AMC, University of Amsterdam, Amsterdam, The Netherlands. Electronic address: m.keizer@sanquin.nl.
authors.forename: Mischa P
authors.initials: MP
authors.lastname: Keizer
authors.affiliation: Department of Immunopathology, Sanquin Research and Landsteiner Laboratory, AMC, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Angela M
authors.initials: AM
authors.lastname: Kamp
authors.affiliation: Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, AMC, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Nannette
authors.initials: N
authors.lastname: Brouwer
authors.affiliation: Emma Children's Hospital, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Marianne D
authors.initials: MD
authors.lastname: van de Wetering
authors.affiliation: Department of Immunopathology, Sanquin Research and Landsteiner Laboratory, AMC, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Diana
authors.initials: D
authors.lastname: Wouters
authors.affiliation: Emma Children's Hospital, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands; Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, AMC, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Taco W
authors.initials: TW
authors.lastname: Kuijpers
mesh_terms: D001798:Blood Proteins / Q000378:metabolism; D002648:Child; D050718:Complement C1 Inhibitor Protein / Q000378:metabolism*; D003181:Complement C4 / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D037241:Mannose-Binding Lectins / Q000172:deficiency / Q000378:metabolism*; D050606:Mannose-Binding Protein-Associated Serine Proteases / Q000378:metabolism; D046912:Multiprotein Complexes / Q000378:metabolism; D011485:Protein Binding; D017186:Titrimetry
chemical_list: D001798:Blood Proteins; D050718:Complement C1 Inhibitor Protein; D003181:Complement C4; D037241:Mannose-Binding Lectins; D046912:Multiprotein Complexes; C105296:MASP2 protein, human; D050606:Mannose-Binding Protein-Associated Serine Proteases
keywords: AP; C1-inh; C1-inhibitor; CP; Functionality; HPE; LP; MASPs; MBL; MBL associated serine proteases; MBL-substitution; MBL/MASP/C1-inh; MMC; Mass-Spec; Plasma-derived MBL; Pre-activated MASPs; TMB; TP; alternative pathway; classical pathway; high performance ELISA buffer; lectin pathway; mannan binding lectin; mass-spectrometry; pAb; pdMBL; plasma-derived MBL; polyHRP; polyclonal antibodies; polymerized Horseradish peroxidase; recombinant human MASP-2; recombinant human MBL; rhMASP-2; rhMBL; terminal pathway; tetramethylbenzidine