title: Distinct iPS Cells Show Different Cardiac Differentiation Efficiency.
abstract: Patient-specific induced pluripotent stem (iPS) cells can be generated by introducing transcription factors that are highly expressed in embryonic stem (ES) cells into somatic cells. This opens up new possibilities for cell transplantation-based regenerative medicine by overcoming the ethical issues and immunological problems associated with ES cells. Despite the development of various methods for the generation of iPS cells that have resulted in increased efficiency, safety, and general versatility, it remains unknown which types of iPS cells are suitable for clinical use. Therefore, the aims of the present study were to assess (1) the differentiation potential, time course, and efficiency of different types of iPS cell lines to differentiate into cardiomyocytes in vitro and (2) the properties of the iPS cell-derived cardiomyocytes. We found that high-quality iPS cells exhibited better cardiomyocyte differentiation in terms of the time course and efficiency of differentiation than low-quality iPS cells, which hardly ever differentiated into cardiomyocytes. Because of the different properties of the various iPS cell lines such as cardiac differentiation efficiency and potential safety hazards, newly established iPS cell lines must be characterized prior to their use in cardiac regenerative medicine.
authors.affiliation: Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Ohno
authors.affiliation: Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Yuasa
authors.affiliation: Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Egashira
authors.affiliation: Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Seki
authors.affiliation: Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Hashimoto
authors.affiliation: Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Shugo
authors.initials: S
authors.lastname: Tohyama
authors.affiliation: Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Saito
authors.affiliation: Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Kunitomi
authors.affiliation: Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Shimoji
authors.affiliation: Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Onizuka
authors.affiliation: Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Toshimi
authors.initials: T
authors.lastname: Kageyama
authors.affiliation: Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Kojiro
authors.initials: K
authors.lastname: Yae
authors.affiliation: Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, Japan ; Biomedical Research Laboratories, Asubio Pharma Co., Ltd., Kobe 650-0047, Japan.
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Ruri
authors.initials: R
authors.lastname: Kaneda
authors.affiliation: Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, Japan ; Biomedical Research Laboratories, Asubio Pharma Co., Ltd., Kobe 650-0047, Japan.
authors.forename: Fumiyuki
authors.initials: F
authors.lastname: Hattori
authors.affiliation: Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Mitsushige
authors.initials: M
authors.lastname: Murata
authors.affiliation: Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Kensuke
authors.initials: K
authors.lastname: Kimura
authors.affiliation: Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Fukuda