title: Utilization of glycerin byproduct derived from soybean oil biodiesel as a carbon source for heterologous protein production in Pichia pastoris.
abstract: Crude glycerol, also known as glycerin, is the main byproduct of the biodiesel industry. It has been estimated that up to 40,000 tons of glycerin will be produced each year by 2020. This study evaluated the value-added use of crude glycerol derived from soybean biodiesel preparation as a carbon source for heterologous protein production using the yeast Pichia pastoris. Eleven glycerin samples were obtained by methanolysis of soybean oil using different acids or bases as catalysts. Cell growth experiments showed that crude glycerol containing either potassium or sodium hydroxide resulted in 1.5-2 times higher final cell densities when compared to glycerol P.A. Finally, crude glycerol containing sodium hydroxide was successfully utilized for constitutive heterologous α-amylase production in P. pastoris. This study demonstrated that crude glycerol without any purification steps may be directly used as carbon source for protein production in P. pastoris.
authors.affiliation: Centro de Biotecnologia Molecular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF 70910-900, Brazil.
authors.forename: G S
authors.initials: GS
authors.lastname: Anastácio
authors.affiliation: Laboratório de Materiais e Combustíveis, Instituto de Química, Universidade de Brasília, Brasília, DF 70910-000, Brazil.
authors.forename: K O
authors.initials: KO
authors.lastname: Santos
authors.affiliation: Laboratório de Materiais e Combustíveis, Instituto de Química, Universidade de Brasília, Brasília, DF 70910-000, Brazil.
authors.forename: P A Z
authors.initials: PA
authors.lastname: Suarez
authors.affiliation: Centro de Biotecnologia Molecular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF 70910-900, Brazil.
authors.forename: F A G
authors.initials: FA
authors.lastname: Torres
authors.affiliation: Laboratório de Materiais e Combustíveis, Instituto de Química, Universidade de Brasília, Brasília, DF 70910-000, Brazil.
authors.forename: J L
authors.initials: JL
authors.lastname: De Marco
authors.affiliation: Centro de Biotecnologia Molecular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF 70910-900, Brazil; Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, DF 70790-160, Brazil. Electronic address: nadiasp@unb.br.
authors.forename: N S
authors.initials: NS
authors.lastname: Parachin
mesh_terms: D000332:Aerobiosis / Q000187:drug effects; D001412:Bacillus subtilis / Q000187:drug effects / Q000201:enzymology; D056804:Biofuels; D002244:Carbon / Q000494:pharmacology*; D002384:Catalysis / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation / Q000187:drug effects; D005990:Glycerol / Q000494:pharmacology*; D000432:Methanol / Q000494:pharmacology; D010843:Pichia / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D013024:Soybean Oil / Q000737:chemistry*; D000516:alpha-Amylases / Q000096:biosynthesis*
chemical_list: D056804:Biofuels; D002244:Carbon; D013024:Soybean Oil; D000516:alpha-Amylases; D005990:Glycerol; D000432:Methanol
keywords: Crude glycerol; Fermentation; Heterologous expression; Pichia pastoris