title: Time-resolved surface-enhanced IR-absorption spectroscopy of direct electron transfer to cytochrome c oxidase from R. sphaeroides.
abstract: Time-resolved surface-enhanced IR-absorption spectroscopy triggered by electrochemical modulation has been performed on cytochrome c oxidase from Rhodobacter sphaeroides. Single bands isolated from a broad band in the amide I region using phase-sensitive detection were attributed to different redox centers. Their absorbances changing on the millisecond timescale could be fitted to a model based on protonation-dependent chemical reaction kinetics established previously. Substantial conformational changes of secondary structures coupled to redox transitions were revealed.
authors.affiliation: Austrian Institute of Technology, Vienna, Austria.
authors.forename: Andreas
authors.initials: A
authors.lastname: Schwaighofer
authors.affiliation: Austrian Institute of Technology, Vienna, Austria.
authors.forename: Christoph
authors.initials: C
authors.lastname: Steininger
authors.affiliation: Austrian Institute of Technology, Vienna, Austria.
authors.forename: David M
authors.initials: DM
authors.lastname: Hildenbrandt
authors.affiliation: Austrian Institute of Technology, Vienna, Austria; Center of Electrochemical Surface Technology, Wiener Neustadt, Austria.
authors.forename: Johannes
authors.initials: J
authors.lastname: Srajer
authors.affiliation: Austrian Institute of Technology, Vienna, Austria; Center of Electrochemical Surface Technology, Wiener Neustadt, Austria.
authors.forename: Christoph
authors.initials: C
authors.lastname: Nowak
authors.affiliation: Austrian Institute of Technology, Vienna, Austria.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Knoll
authors.affiliation: Austrian Institute of Technology, Vienna, Austria. Electronic address: Renate.Naumann@ait.ac.at.
authors.forename: Renate L C
authors.initials: RL
authors.lastname: Naumann
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D004579:Electron Transport; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000378:metabolism*; D004583:Electrons; D012242:Rhodobacter sphaeroides / Q000201:enzymology* / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001426:Bacterial Proteins; D003576:Electron Transport Complex IV