title: Characterisation of acid-soluble and pepsin-solubilised collagen from jellyfish (Cyanea nozakii Kishinouye).
abstract: Annual outbreaks of the Jellyfish (Cyanea nozakii Kishinouye) in the waters of the Yellow Sea and the East China Sea are regarded as a nuisance. Thus, utilizing this jellyfish species is of great significance to reduce harm to fisheries and marine environments. The yield of the acid-soluble collagens (ASCs) from the C. nozakii umbrella was 13.0% (dry weight) and that of the pepsin-solubilised collagens (PSCs) was 5.5% (dry weight). The SDS-PAGE patterns of the ASCs and PSCs differed from that of type I collagen, which indicate the presence of (α1)3. The denaturation temperature (Td) of the collagens was approximately 23.8°C. Fourier transform infrared spectroscopy proved that the ASCs and PSCs retained their helical structures and the As, Pb, and Hg content of the collagens, detected by ICP-MS, were considerably lower than the national standards. The results suggest that collagens isolated from C. nozakii can potentially be used as an alternative source of collagen for use in various applications.
authors.affiliation: School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang 222005, China. Electronic address: zjjdr180@163.com.
authors.forename: Junjie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang 222005, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Duan
authors.affiliation: School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang 222005, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.affiliation: School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang 222005, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Food Science, Cornell University, Ithaca, NY 14853-7201, USA.
authors.forename: Joe M
authors.initials: JM
authors.lastname: Regenstein
mesh_terms: D000818:Animals; D002681:China; D003094:Collagen / Q000737:chemistry*; D029941:Fish Proteins / Q000737:chemistry*; D005511:Food Handling; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008970:Molecular Weight; D010434:Pepsin A / Q000737:chemistry; D011489:Protein Denaturation; D037401:Scyphozoa / Q000737:chemistry*; D012867:Skin / Q000737:chemistry; D012995:Solubility
chemical_list: D029941:Fish Proteins; D003094:Collagen; D010434:Pepsin A
keywords: ASC; Cyanea nozakii; Denaturation temperature; ICP-MS; Infrared spectrum; PSC