title: Virus-induced signaling influences endosome trafficking, virus entry, and replication.
abstract: Viruses are obligate intracellular pathogens that interact with host cell machinery for enabling entry, replication, and spread. This chapter describes methods for studying the interaction of viruses with host cell signaling pathways and surface receptors during cellular infection, with an emphasis on protein kinases. We also describe how use of immunofluorescence confocal microscopy for imaging virus-host interactions provides a powerful approach for obtaining structural correlations that extend results of immunological and biochemical assays.
authors.affiliation: Li Ka Shing Institute of Virology, Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: David J
authors.initials: DJ
authors.lastname: Marchant
authors.affiliation: Li Ka Shing Institute of Virology, Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Leanne
authors.initials: L
authors.lastname: Bilawchuk
authors.affiliation: Li Ka Shing Institute of Virology, Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Gordon
authors.initials: G
authors.lastname: Nish
authors.affiliation: Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada. Electronic address: richard.hegele@utoronto.ca.
authors.forename: Richard G
authors.initials: RG
authors.lastname: Hegele
mesh_terms: D000818:Animals; D015153:Blotting, Western; D011992:Endosomes / Q000378:metabolism* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism* / Q000821:virology; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D054884:Host-Pathogen Interactions; D053118:Influenza A Virus, H1N1 Subtype / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D008856:Microscopy, Fluorescence; D053594:Myeloid Differentiation Factor 88 / Q000172:deficiency / Q000235:genetics; D021381:Protein Transport; D012136:Respiratory Syncytial Viruses / Q000502:physiology*; D015398:Signal Transduction / Q000235:genetics*; D053586:Virus Internalization; D014779:Virus Replication; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism
chemical_list: C507780:Myd88 protein, mouse; D053594:Myeloid Differentiation Factor 88; D049452:Green Fluorescent Proteins; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Cell Signalling; Endocytosis; Virus Entry; Virus Traffickin