title: Effect of Prunetin on TNF-α-Induced MUC5AC Mucin Gene Expression, Production, Degradation of IκB and Translocation of NF-κB p65 in Human Airway Epithelial Cells.
abstract: BACKGROUND
  We investigated whether prunetin significantly affects tumor necrosis factor-α (TNF-α)-induced MUC5AC mucin gene expression, production, inhibitory kappa B (IκB) degradation and nuclear factor kappa B (NF-κB) p65 translocation in human airway epithelial cells.
  
  
  METHODS
  Confluent NCI-H292 cells were pretreated with prunetin for 30 minutes and then stimulated with TNF-α for 24 hours or the indicated periods. MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. The effect of prunetin on TNF-α-induced degradation of IκB and translocation of NF-κB p65 was investigated by western blot analysis.
  
  
  RESULTS
  We found that incubation of NCI-H292 cells with prunetin significantly inhibited mucin production and down-regulated the MUC5AC gene expression induced by TNF-α. Prunetin inhibited TNF-α-induced degradation of IκB and translocation of NF-κB p65.
  
  
  CONCLUSIONS
  This result suggests that prunetin inhibits the NF-κB signaling pathway, which may explain its role in the inhibition of MUC5AC mucin gene expression and production regulated by the NF-κB signaling pathway.
authors.affiliation: Department of Pharmacology, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Jiho
authors.initials: J
authors.lastname: Ryu
authors.affiliation: Department of Pharmacology, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Hyun Jae
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Pharmacology, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Su Hyun
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Pharmacology, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Md Asaduzzaman
authors.initials: MA
authors.lastname: Sikder
authors.affiliation: Department of Pharmacology, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon, Korea. ; Pulmonology Section, Department of Internal Medicine, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Ju-Ock
authors.initials: JO
authors.lastname: Kim
authors.affiliation: Department of Pharmacology, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Jang-Hee
authors.initials: JH
authors.lastname: Hong
authors.affiliation: Department of Pharmacology, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Jeong Ho
authors.initials: JH
authors.lastname: Seok
authors.affiliation: Department of Pharmacology, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Choong Jae
authors.initials: CJ
authors.lastname: Lee
keywords: MUC5AC Protein, Human; Mucins; Prunetin