title: Chromatographic methods for characterization of poly(ethylene glycol)-modified polyamidoamine dendrimers.
abstract: The objective of this study was to develop chromatographic methods for the determination of the modification degree and the characterization of poly(ethylene glycol)-modified polyamidoamine dendrimers (PEG-PAMAMs). The PEG-PAMAMs were prepared by reacting PAMAM generation 4 with monomethoxy PEG-nitrophenyl carbonate (mPEG-NPC). The modification degrees of PEG-PAMAMs were determined by quantifying 4-nitrophenol released from mPEG-NPC after PEGylation reaction using high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The PEG-PAMAMs, which have poor UV absorbances, were characterized by HPLC with charged aerosol detection. This study demonstrates that the combination of these two detectors is a powerful tool for the preparation and characterization of PEG-PAMAMs.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Eun Ji
authors.initials: EJ
authors.lastname: Park
authors.affiliation: Department of Polymer Science and Engineering, Chosun University, Gwangju 501-759, Republic of Korea.
authors.forename: Hoon
authors.initials: H
authors.lastname: Cho
authors.affiliation: Department of Environmental Engineering, Chosun University, Gwangju 501-759, Republic of Korea.
authors.forename: Si Wouk
authors.initials: SW
authors.lastname: Kim
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea. Electronic address: dhna@knu.ac.kr.
authors.forename: Dong Hee
authors.initials: DH
authors.lastname: Na
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D050091:Dendrimers / Q000737:chemistry*; D009596:Nitrophenols / Q000032:analysis; D011073:Polyamines / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000032:analysis*
chemical_list: D050091:Dendrimers; D009596:Nitrophenols; C531249:Poly(amidoamine); D011073:Polyamines; D011092:Polyethylene Glycols; C024836:4-nitrophenol
keywords: Charged aerosol detection; Chromatographic method; PEGylation; Polyamidoamine dendrimers