title: Characterisation of the soil bacterial community structure and composition of natural and constructed wetlands.
abstract: In the present study, the pyrosequencing of 16S ribosomal DNA was used to characterise the soil bacterial community composition of a constructed wetland receiving municipal wastewater and a nearby natural wetland. Soil samples were taken from different locations in each wetland (lagoon, zone with T. latifolia, zone with S. atrocinerea). Moreover, the water quality parameters were evaluated (pH, Tª, conductivity, dissolved oxygen, redox potential, nutrients and suspended solids), revealing that the organic matter and nutrient contents were significantly higher in the constructed wetland than in the natural one. In general, the bacterial communities of the natural wetland were more diverse than those of the constructed wetland. The major phylogenic groups of all soils included Proteobacteria, Verrucomicrobia and Chloroflexi, with Proteobacteria being the majority of the community composition. The Verrucomicrobia and Chloroflexi phyla were more abundant in the natural wetland than the constructed wetland; in contrast, the Proteobacteria phylum was more abundant in the constructed wetland than the natural wetland. Beta diversity analyses reveal that the soil bacterial communities in the natural wetland were less dissimilar to each other than to those of the constructed wetland.
authors.affiliation: Departamento de Biodiversidad y Gestión Ambiental, Universidad de León. Campus de Vegazana s/n, CP: 24071, León, Spain. Electronic address: gemma.ansola@unileon.es.
authors.forename: Gemma
authors.initials: G
authors.lastname: Ansola
authors.affiliation: Instituto de Medioambiente, Recursos Naturales y Biodiversidad, Universidad de León. Calle La Serna, no. 56, CP: 24071, León, Spain. Electronic address: parrh@unileon.es.
authors.forename: Paula
authors.initials: P
authors.lastname: Arroyo
authors.affiliation: Departamento de Biología Molecular, Universidad de León. Campus de Vegazana s/n, CP: 24071, León, Spain. Electronic address: luis.saenzdemiera@unileon.es.
authors.forename: Luis E
authors.initials: LE
authors.lastname: Sáenz de Miera
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000254:growth & development; D052918:Environmental Restoration and Remediation; D012987:Soil; D012988:Soil Microbiology; D014865:Waste Disposal, Fluid / Q000379:methods; D062065:Wastewater / Q000382:microbiology; D053833:Wetlands
chemical_list: D012987:Soil; D062065:Wastewater
keywords: 16S rRNA pyrosequencing; Constructed wetlands; Natural wetlands; Quality water; Soil bacterial community