title: [Characteristics and functional protein analysis of an effective decabromodiphenyl ether-degrading strain].
abstract: An effective decabromodiphenyl ether (BDE-209) degrading strain was isolated and identified as Enterococcus casseliflavus based on the 16S rRNA gene sequence analysis. The optimal conditions for strain growth were pH 7 and culture time of 48 h, respectively. E. casseliflavus has a good ability to degrade BDE-209. The biodegradation rate of 1 mg.L-1 BDE-209 by 1 g.L-1 E. casseliflavus reached the highest of 56. 7% after 4 days degradation with 5 mg.L-1 glucose as the additional carbon source. During the degradation process of BDE-209, SDS-PAGE demonstrated that some new extracellular proteins were induced under 2 mg.L-1 and 5 mg.L-1 BDE-209. As for the intracellular proteins, the quantity of protein expression varied, and some proteins even disappeared compared with the blank control. Two-dimensional electrophoresis steps for protein analysis detected 31 different protein points, demonstrating that during the degradation process, the conformation of some proteins which were related with degradation was changed, and resulted in the variation of type and content of the proteins.
authors.affiliation: Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, Department of Environmental Engineering, Jinan University, Guangzhou 510632, China. 412108631@qq.com
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Chang
authors.affiliation: Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, College of Environment and Energy, South China University of Technology, Guangzhou 510006, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yin
authors.affiliation: Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, Department of Environmental Engineering, Jinan University, Guangzhou 510632, China.
authors.forename: Hua-Ming
authors.initials: HM
authors.lastname: Qin
authors.affiliation: Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, Department of Environmental Engineering, Jinan University, Guangzhou 510632, China.
authors.forename: Jin-Shao
authors.initials: JS
authors.lastname: Ye
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Peng
authors.affiliation: Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, College of Environment and Energy, South China University of Technology, Guangzhou 510006, China.
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Song
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D001673:Biodegradation, Environmental; D004591:Electrophoresis, Polyacrylamide Gel; D016983:Enterococcus / Q000145:classification* / Q000235:genetics; D055768:Halogenated Diphenyl Ethers / Q000737:chemistry*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D055768:Halogenated Diphenyl Ethers; D012336:RNA, Ribosomal, 16S; C010902:decabromobiphenyl ether