title: Single-molecule force spectroscopy study on the mechanism of RNA disassembly in tobacco mosaic virus.
abstract: To explore the disassembly mechanism of tobacco mosaic virus (TMV), a model system for virus study, during infection, we have used single-molecule force spectroscopy to mimic and follow the process of RNA disassembly from the protein coat of TMV by the replisome (molecular motor) in vivo, under different pH and Ca(2+) concentrations. Dynamic force spectroscopy revealed the unbinding free-energy landscapes as that at pH 4.7 the disassembly process is dominated by one free-energy barrier, whereas at pH 7.0 the process is dominated by one barrier and that there exists a second barrier. The additional free-energy barrier at longer distance has been attributed to the hindrance of disordered loops within the inner channel of TMV, and the biological function of those protein loops was discussed. The combination of pH increase and Ca(2+) concentration drop could weaken RNA-protein interactions so much that the molecular motor replisome would be able to pull and disassemble the rest of the genetic RNA from the protein coat in vivo. All these facts provide supporting evidence at the single-molecule level, to our knowledge for the first time, for the cotranslational disassembly mechanism during TMV infection under physiological conditions.
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, P. R. China; School of Chemistry and Material Science, Liaoning Shihua University, Fushun, P. R. China.
authors.forename: Ningning
authors.initials: N
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, P. R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, P. R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, P. R. China; Department of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina. Electronic address: wang@mail.chem.sc.edu.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, P. R. China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Su
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, P. R. China. Electronic address: zhangwk@jlu.edu.cn.
authors.forename: Wenke
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, P. R. China; Department of Chemistry, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, P. R. China.
authors.forename: Jiacong
authors.initials: J
authors.lastname: Shen
mesh_terms: D000595:Amino Acid Sequence; D002118:Calcium / Q000737:chemistry; D036022:Capsid Proteins / Q000737:chemistry / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D018625:Microscopy, Atomic Force; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D012367:RNA, Viral / Q000737:chemistry* / Q000378:metabolism; D013057:Spectrum Analysis; D014027:Tobacco Mosaic Virus / Q000737:chemistry* / Q000378:metabolism; D000099272:Replisomes
chemical_list: D002118:Calcium; D036022:Capsid Proteins; D004259:DNA-Directed DNA Polymerase; D009097:Multienzyme Complexes; D012367:RNA, Viral; D000099272:Replisomes