title: Interactions between endosomal maturation and autophagy: analysis of ESCRT machinery during Caenorhabditis elegans development.
abstract: Endocytosis and autophagy are key vesicular pathways involved in degradation and recycling of cellular material. Both degradative pathways finally fuse with lysosome but are indeed interconnected at several levels. In particular, the fusion between endosomes and autophagosomes can generate intermediate vesicles named amphisomes. We analyzed the physiological and developmental roles of the ESCRT machinery in a model organism, the nematode Caenorhabditis elegans and showed that the blockage of the endosomal maturation triggers the induction of autophagic activity. This chapter describes several methods for studying endocytosis, autophagy, and their interconnection in C. elegans. A series of genetic, biochemical, and microscopy analyses has been used to study at the cellular and developmental levels, the cross talks between autophagy and endocytosis.
authors.affiliation: Centre de Génétique Moléculaire, CNRS UPR3404, Université Paris-Sud, 91198 Gif-sur-Yvette Cedex, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Manil-Ségalen
authors.affiliation: Centre de Génétique Moléculaire, CNRS UPR3404, Université Paris-Sud, 91198 Gif-sur-Yvette Cedex, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Culetto
authors.affiliation: Centre de Génétique Moléculaire, CNRS UPR3404, Université Paris-Sud, 91198 Gif-sur-Yvette Cedex, France. Electronic address: legouis@cgm.cnrs-gif.fr.
authors.forename: Renaud
authors.initials: R
authors.lastname: Legouis
authors.affiliation: Centre de Génétique Moléculaire, CNRS UPR3404, Université Paris-Sud, 91198 Gif-sur-Yvette Cedex, France. Electronic address: lefebvre@cgm.cnrs-gif.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: Lefebvre
mesh_terms: D000818:Animals; D001343:Autophagy / Q000235:genetics*; D017173:Caenorhabditis elegans / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D004705:Endocytosis / Q000235:genetics*; D056827:Endosomal Sorting Complexes Required for Transport / Q000235:genetics / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism* / Q000648:ultrastructure; D018507:Gene Expression Regulation, Developmental; D020868:Gene Silencing; D005822:Genetic Vectors; D008247:Lysosomes / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D010588:Phagosomes / Q000378:metabolism* / Q000648:ultrastructure; D015398:Signal Transduction; D019076:Transgenes
chemical_list: D029742:Caenorhabditis elegans Proteins; D056827:Endosomal Sorting Complexes Required for Transport
keywords: Amphisomes; Autophagosomes; C. elegans; ESCRT; Endosomes; Immunofluorescence; Transgenesis; Ubiquitin-like proteins