title: Impact of NPM, TFF3 and TACC1 on the prognosis of patients with primary gastric cancer.
abstract: BACKGROUND
  NPM, TFF3 and TACC1 are molecular markers that play important roles in cell differentiation. Herein, we investigated their prognostic impact in patients with primary gastric cancer (GC) and determined whether they could be used as markers of more aggressive gastric carcinomas by detecting the extent of expression in human gastric carcinoma samples.
  
  
  RESULTS
  Tumor tissue specimens from 142 GC patients were retrospectively retrieved and immunohistochemically evaluated. Correlations between NPM, TFF3 and TACC1 over-expression and clinicopathologic parameters, and their prognostic values were investigated with χ(2), Kaplan-Meier method, and Cox uni- and multivariate survival models. NPM, TFF3 and TACC1 expression was significantly higher in GC patients with poorly differentiated histologic type than that in patients with well differentiated histologic type. NPM expression was significantly higher in patients with hepatic metastasis or recurrence than that in patients without metastasis. TFF3 expression was significantly higher in patients with positive lymph node metastasis than that in patients with negative lymph node metastasis. Age, lymph node metastasis, and TFF3 and TACC1 over-expression were significantly correlated with low survival (P<0.05, P<0.05, P = 0.005 and P = 0.009, respectively). Multivariate analysis showed that lymph node metastasis and TFF3 and TACC1 over-expression were independent prognostic factors.
  
  
  CONCLUSIONS
  TFF3 and TACC1 over-expression in epithelial cells of surgically resected GC tissues was an independent predictor of short survival in GC patients. The prognosis was poorer in patients with positive expression of both TFF3 and TACC1 than that in patients with positive expression of TFF3 or TACC1 alone, or with negative expression of TFF3 and TACC1.
authors.affiliation: Department of Oncology, Affiliated Hospital of Qingdao University School of Medicine, Qingdao, People's Republic of China.
authors.forename: Aiping
authors.initials: A
authors.lastname: Ding
authors.affiliation: Department of Oncology, Affiliated Hospital of Qingdao University School of Medicine, Qingdao, People's Republic of China ; Qingdao Cancer Hospital, Shibei City, Qingdao, Shandong Province, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Oncology, Affiliated Hospital of Qingdao University School of Medicine, Qingdao, People's Republic of China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Shi
authors.affiliation: Central Laboratory, Affiliated Hospital of Qingdao University School of Medicine, Qingdao, People's Republic of China.
authors.forename: Ruyong
authors.initials: R
authors.lastname: Yao
authors.affiliation: Department of Oncology, Affiliated Hospital of Qingdao University School of Medicine, Qingdao, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhou
authors.affiliation: Department of Oncology, Affiliated Hospital of Qingdao University School of Medicine, Qingdao, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yue
authors.affiliation: Central Laboratory, Affiliated Hospital of Qingdao University School of Medicine, Qingdao, People's Republic of China.
authors.forename: Shihai
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Oncology, Affiliated Hospital of Qingdao University School of Medicine, Qingdao, People's Republic of China.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Qiu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D005260:Female; D005326:Fetal Proteins / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008875:Middle Aged; D009687:Nuclear Proteins / Q000378:metabolism*; D000090243:Nucleophosmin; D010455:Peptides / Q000378:metabolism*; D011379:Prognosis; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D015996:Survival Rate; D000071165:Trefoil Factor-3
chemical_list: D014408:Biomarkers, Tumor; D005326:Fetal Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D010455:Peptides; C120584:TACC1 protein, human; C082495:TFF3 protein, human; D000071165:Trefoil Factor-3; D000090243:Nucleophosmin