title: Image-based single-cell sorting via dual-photopolymerized microwell arrays.
abstract: We present a simple and cost-effective single-cell sorting method using two sequential photopolymerization steps that enables sorting based upon imaged phenotypes. The first photopolymerization step uses a thiolene-based resin with minimal autofluorescence to create an array of microwells to capture cells. The second photopolymerization uses (poly)ethylene glycol diacrylate to encapsulate undesired cells in a hydrogel, allowing for retrieval of the desired cell population using simple washing. We quantitatively characterize the method using fluorescently labeled cells and then applied the method to isolate cells based upon imaged fluorescence localization. The method is readily transferrable to other laboratories and will provide a facile route to sorting of cells based on imaged phenotypes.
authors.affiliation: Research Laboratory of Electronics, Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology , 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.forename: Joseph
authors.initials: J
authors.lastname: Kovac
authors.forename: Joel
authors.initials: J
authors.lastname: Voldman
mesh_terms: D002469:Cell Separation / Q000191:economics / Q000379:methods*; D002470:Cell Survival; D015870:Gene Expression; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D020100:Hydrogels; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods; D055668:Photochemical Processes; D011092:Polyethylene Glycols / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry; D059010:Single-Cell Analysis / Q000191:economics / Q000379:methods*; D014466:Ultraviolet Rays; D000097573:Red Fluorescent Protein
chemical_list: D049452:Green Fluorescent Proteins; D020100:Hydrogels; D008164:Luminescent Proteins; D011092:Polyethylene Glycols; D012117:Resins, Synthetic; D000097573:Red Fluorescent Protein; C437167:poly(ethylene glycol)diacrylate