title: Aberrant microRNA expression in endometrial carcinoma using formalin-fixed paraffin-embedded (FFPE) tissues.
abstract: This study aimed to identify the candidate miRNAs in the carcinogenesis of endometrial carcinoma, and to explore whether FFPE material would be suitable for miRNA profiling. We identified the differences between miRNA expression profiles using human miRNA microarray in endometrioid endometrial adenocarcinomas (EECs) and normal endometria. Of those tested, miR-200a*, miR-200b*, miR-141, miR-182, and miR-205 were greatly enriched. The expressions of these five miRNAs were validated using quantitative real-time reverse transcription-PCR (qRT-PCR). We then performed qRT-PCR miR expression profiling in 30 FFPE specimens (20 EECs, 10 normal endometria) and re-confirmed the results of differential expression between cancer and normal tissue. Following this, we tested whether the specific inhibition of overexpressed miRNAs would alter chemosensitivity. In the in vitro cell viability assay, anti-miR200b* showed a trend toward enhanced cytotoxicity slightly in cisplatin compared to the negative control (p = 0.07). This information provided the candidate miRNAs for further confirmation of the role of miRNAs in the carcinogenesis of EECs, potentially serving as a diagnostic or therapeutic tool. FFPE specimens of endometrial tissues are suitable as a source for miRNA microarray profiling.
authors.affiliation: Department of Obstetrics and Gynecology, SMG-SNU Boramae Medical Center, Seoul, Korea.
authors.forename: Taek Sang
authors.initials: TS
authors.lastname: Lee
authors.affiliation: Department of Obstetrics and Gynecology, SMG-SNU Boramae Medical Center, Seoul, Korea.
authors.forename: Hye Won
authors.initials: HW
authors.lastname: Jeon
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul National University Bundang Hospital, Seongnam, Korea.
authors.forename: Yong Beom
authors.initials: YB
authors.lastname: Kim
authors.affiliation: Department of Pathology, SMG-SNU Boramae Medical Center, Seoul Korea.
authors.forename: Young A
authors.initials: YA
authors.lastname: Kim
authors.affiliation: Department of Pathology, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Min A
authors.initials: MA
authors.lastname: Kim
authors.affiliation: Department of Obstetrics and Gynecology, Konkuk University Medical Center, Seoul, Korea.
authors.forename: Soon Beom
authors.initials: SB
authors.lastname: Kang
mesh_terms: D063646:Carcinogenesis / Q000235:genetics*; D018269:Carcinoma, Endometrioid / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005557:Formaldehyde; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D046228:Microarray Analysis; D016612:Paraffin Embedding; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric
chemical_list: D017931:DNA Primers; D035683:MicroRNAs; D005557:Formaldehyde