title: Comparing different sterol containing solid lipid nanoparticles for targeted delivery of quercetin in hepatocellular carcinoma.
abstract: Quercetin (QT) is a potential chemotherapeutic drug with low solubility that seriously limits its clinical use. The aim of this study was enhancing cellular penetration of QT by sterol containing solid lipid nanoparticles (SLNs) which make bilayers fluent for targeting hepatocellular carcinoma cells. Three variables including sterol type (cholesterol, stigmasterol and stigmastanol), drug and sterol content were studied in a surface response D-optimal design for preparation of QT-SLNs by emulsification solvent evaporation method. The studied responses included particle size, zeta potential, drug loading capacity and 24 h release efficiency (RE24%). Scanning electron and atomic force microscopy were used to study the morphology of QT-SLNs and their thermal behavior was studied by DSC analysis. Cytotoxicity of QT-SLNs was determined by MTT assay on HepG-2 cells and cellular uptake by fluorescence microscopy method. Optimized QT-SLNs obtained from cholesterol and QT with the ratio of 2:1 that showed particle size of 78.0 ± 7.0 nm, zeta potential of -22.7 ± 1.3 mV, drug loading efficiency of 99.9 ± 0.5% and RE24 of 56.3 ± 3.4%. IC50 of QT in cholesterol SLNs was about six and two times less than free QT and phytosterol SLNs, respectively, and caused more accumulation of QT in HepG2 cells. Blank phytosterol SLNs were toxic on cells.
authors.affiliation: Department of Pharmaceutics, Faculty of Pharmacy and Novel Drug Delivery Systems Research Center, Isfahan University of Medical Sciences , Isfahan , Islamic Republic of Iran.
authors.forename: Jaleh
authors.initials: J
authors.lastname: Varshosaz
authors.forename: Abbas
authors.initials: A
authors.lastname: Jafarian
authors.forename: Golnaz
authors.initials: G
authors.lastname: Salehi
authors.forename: Behzad
authors.initials: B
authors.lastname: Zolfaghari
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage* / Q000493:pharmacokinetics; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002626:Chemistry, Pharmaceutical; D002784:Cholesterol / Q000008:administration & dosage*; D056945:Hep G2 Cells; D006801:Humans; D008081:Liposomes; D008113:Liver Neoplasms / Q000503:physiopathology*; D053758:Nanoparticles; D010316:Particle Size; D011794:Quercetin / Q000008:administration & dosage* / Q000493:pharmacokinetics; D012855:Sitosterols / Q000008:administration & dosage; D013265:Stigmasterol / Q000008:administration & dosage
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D008081:Liposomes; D012855:Sitosterols; D002784:Cholesterol; D013265:Stigmasterol; D011794:Quercetin; C021255:stigmastanol
keywords: Cholesterol; hepatocellular carcinoma; phytosterols; quercetin; solid lipid nanoparticles