title: Mechanism and kinetics modeling of the enzymatic hydrolysis of α1-32 antibacterial peptide.
abstract: Several antibacterial peptides can be obtained by enzymatic hydrolysis of the α chain of bovine hemoglobin. The kinetics of α1-32 peptide hydrolysis by pepsin was studied at several temperatures (15, 23 and 37 °C). Intermediate and final peptides were identified, and their antibacterial activity was assessed against four bacterial species. Evolution of generated peptides concentration enabled to propose a reaction pathway describing the parallel and consecutive reactions taking place during the hydrolysis. A mathematical model, based on the proposed mechanism, was developed to describe the kinetics of generated peptides during α1-32 hydrolysis. The constants of the main reactions were identified based on the experimental data, and their dependence on temperature was established using Arrhenius-type equations. Validation of the proposed model was performed by predicting kinetics of α1-32 peptide hydrolysis at 30 °C (all other experimental conditions being unchanged) with a good accuracy. This mathematical model could allow defining the optimal conditions for the production of various intermediate peptides with antibacterial activity from peptic hydrolysis of α1-32 peptide.
authors.affiliation: Laboratoire ProBioGEM, EA 1026, Polytech'Lille, Université Lille Nord de France, Avenue Paul Langevin, 59655, Villeneuve d'Ascq, France.
authors.forename: K
authors.initials: K
authors.lastname: Hedhili
authors.forename: P
authors.initials: P
authors.lastname: Vauchel
authors.forename: K
authors.initials: K
authors.lastname: Dimitrov
authors.forename: K
authors.initials: K
authors.lastname: Kriaa
authors.forename: G
authors.initials: G
authors.lastname: Chataigné
authors.forename: K
authors.initials: K
authors.lastname: Hani
authors.forename: P
authors.initials: P
authors.lastname: Dhulster
authors.forename: N
authors.initials: N
authors.lastname: Nedjar-Arroume
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D006454:Hemoglobins / Q000737:chemistry*; D006868:Hydrolysis; D008962:Models, Theoretical; D010446:Peptide Fragments / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013696:Temperature
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D006454:Hemoglobins; D010446:Peptide Fragments; D010455:Peptides; D012997:Solvents; C000595309:hemoglobin alpha-chain (1-32), bovine