title: Urogenital mycoplasmas and human papilloma virus in hemodialysed women.
abstract: Bacterial infections, especially endogenous, are the frequent complications among hemodialyzed and renal transplant patients. In this study we assumed the prevalence of urogenital mycoplasmas and HPV among hemodialysed women. We examined 32 hemodialysed women aged 20-48 (mean 35.6 ± 8.23) and 100 healthy controls of the same ages. Two swabs were collected for detection of mycoplasmas and HPV. Culture of Ureaplasma spp. and M. hominis was performed using Mycoplasma IST2 (bioMérieux, France), Identificaton of U. parvum and U. urealyticum was performed by Kong. Primers described by Jensen were used for M. genitalium. For detection of high-risk HPV types Amplicor HPV (Roche Molecular System, CA) was used. Prevalence of urogenital mycoplasmas in the hemodialysed women (53.1%) was significantly higher (P = 0.0059), compared with controls (25%). In both groups, U. parvum was the most frequently isolated. Cooccurrence of urogenital mycoplasmas was shown in 75% of the HPV-positive hemodialysed women and in 30.4% of HPV-positive controls (P = 0.0461). Cooccurrence of urogenital mycoplasmas with HPV was significantly higher in hemodialysed women. The need to take into account these microorganisms in routine diagnostic, especially for hemodialysed patients, was demonstrated. Further studies to demonstrate the role of this cooccurrence in etiopathogenesis of infection in hemodialysed patients are required.
authors.affiliation: Department of Medical Microbiology, Medical University of Silesia, Medyków 18, 40-752 Katowice, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Ekiel
authors.affiliation: Department of Obstetrics and Gynecology, Medical University of Warsaw, Starynkiewicza 1/3, 02-015 Warsaw, Poland.
authors.forename: Bronisława
authors.initials: B
authors.lastname: Pietrzak
authors.affiliation: Department of Medical Microbiology, Medical University of Silesia, Medyków 18, 40-752 Katowice, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Wiechuła
authors.affiliation: Department of Medical Microbiology, Medical University of Silesia, Medyków 18, 40-752 Katowice, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Aptekorz
authors.affiliation: Department of Obstetrics and Gynecology, Medical University of Warsaw, Starynkiewicza 1/3, 02-015 Warsaw, Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Mazanowska
authors.affiliation: Department of Medical Microbiology, Medical University of Silesia, Medyków 18, 40-752 Katowice, Poland.
authors.forename: Dominika
authors.initials: D
authors.lastname: Rady
authors.affiliation: Department of Medical Microbiology, Medical University of Silesia, Medyków 18, 40-752 Katowice, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Kamiński
authors.affiliation: Department of Medical Microbiology, Medical University of Silesia, Medyków 18, 40-752 Katowice, Poland ; Department of Histology and Embryology, Medical University of Warsaw, Chalubińskiego 5, 02-004 Warszawa, Poland.
authors.forename: Gayane
authors.initials: G
authors.lastname: Martirosian
mesh_terms: D000328:Adult; D015897:Comorbidity; D005260:Female; D052776:Female Urogenital Diseases / Q000453:epidemiology / Q000382:microbiology* / Q000821:virology*; D006801:Humans; D008875:Middle Aged; D009174:Mycoplasma / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000453:epidemiology / Q000382:microbiology*; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000382:microbiology*; D011044:Poland / Q000453:epidemiology; D015995:Prevalence; D006435:Renal Dialysis; D012307:Risk Factors; D014566:Urogenital System; D055815:Young Adult