title: Beads-based electrochemical assay for the detection of influenza hemagglutinin labeled with CdTe quantum dots.
abstract: In this study we describe a beads-based assay for rapid, sensitive and specific isolation and detection of influenza vaccine hemagglutinin (HA). Amplification of the hemagglutinin signal resulted from binding of an electrochemical label as quantum dots (QDs). For detection of the metal and protein part of the resulting HA-CdTe complex, two differential pulse voltammetric methods were used. The procedure includes automated robotic isolation and electrochemical analysis of the isolated product. The isolation procedure was based on the binding of paramagnetic particles (MPs) with glycan (Gly), where glycan was used as the specific receptor for linkage of the QD-labeled hemagglutinin.
authors.forename: Ludmila
authors.initials: L
authors.lastname: Krejcova
authors.forename: Lukas
authors.initials: L
authors.lastname: Nejdl
authors.forename: David
authors.initials: D
authors.lastname: Hynek
authors.forename: Sona
authors.initials: S
authors.lastname: Krizkova
authors.forename: Pavel
authors.initials: P
authors.lastname: Kopel
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. kizek@sci.muni.cz.
authors.forename: Rene
authors.initials: R
authors.lastname: Kizek
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry*; D006801:Humans; D007252:Influenza Vaccines; D045663:Quantum Dots / Q000737:chemistry*; D013194:Staining and Labeling; D013691:Tellurium / Q000737:chemistry*
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D013691:Tellurium