title: The roles of the catalytic and noncatalytic activities of Rpd3L and Rpd3S in the regulation of gene transcription in yeast.
abstract: In budding yeasts, the histone deacetylase Rpd3 resides in two different complexes called Rpd3L (large) and Rpd3S (small) that exert opposing effects on the transcription of meiosis-specific genes. By introducing mutations that disrupt the integrity and function of either Rpd3L or Rpd3S, we show here that Rpd3 function is determined by its association with either of these complexes. Specifically, the catalytic activity of Rpd3S activates the transcription of the two major positive regulators of meiosis, IME1 and IME2, under all growth conditions and activates the transcription of NDT80 only during vegetative growth. In contrast, the effects of Rpd3L depends on nutrients; it represses or activates transcription in the presence or absence of a nitrogen source, respectively. Further, we show that transcriptional activation does not correlate with histone H4 deacetylation, suggesting an effect on a nonhistone protein. Comparison of rpd3-null and catalytic-site point mutants revealed an inhibitory activity that is independent of either the catalytic activity of Rpd3 or the integrity of Rpd3L and Rpd3S.
authors.affiliation: Department of Biology, Technion, Haifa, Israel.
authors.forename: Daniella
authors.initials: D
authors.lastname: Yeheskely-Hayon
authors.affiliation: Department of Biology, Technion, Haifa, Israel.
authors.forename: Anat
authors.initials: A
authors.lastname: Kotler
authors.affiliation: Department of Biology, Technion, Haifa, Israel.
authors.forename: Michal
authors.initials: M
authors.lastname: Stark
authors.affiliation: Department of Biology, Technion, Haifa, Israel.
authors.forename: Tamar
authors.initials: T
authors.lastname: Hashimshony
authors.affiliation: Department of Biology, Technion, Haifa, Israel.
authors.forename: Shira
authors.initials: S
authors.lastname: Sagee
authors.affiliation: Department of Biology, Technion, Haifa, Israel.
authors.forename: Yona
authors.initials: Y
authors.lastname: Kassir
mesh_terms: D000107:Acetylation; D002244:Carbon / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D017353:Gene Deletion; D015870:Gene Expression; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D006657:Histones / Q000378:metabolism; D008540:Meiosis; D011485:Protein Binding; D004718:Saccharomycetales / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D005656:Fungal Proteins; D006657:Histones; D002244:Carbon