title: Pentalinon andrieuxii root extract is effective in the topical treatment of cutaneous leishmaniasis caused by Leishmania mexicana.
abstract: Cutaneous leishmaniasis (CL) manifests as localized skin lesions, which lead to significant tissue destruction and disfigurement. In the Yucatan Peninsula, Mayan traditional healers use Pentalinon andrieuxii Muell.-Arg. (Apocynaceae) roots for the topical treatment of CL. Here, we studied the effect of P. andrieuxii root hexane extract (PARE) on the parasites and host cells in vitro and examined its efficacy in the topical treatment of CL caused by Leishmania mexicana. PARE exhibited potent antiparasitic activity in vitro against promastigotes as well as amastigotes residing in macrophages. Electron microscopy of PARE-treated parasites revealed direct membrane damage. PARE also activated nuclear factor kappaB and enhanced interferon-γ receptor and MHC class II expression and TNF-α production in macrophages. In addition, PARE induced production of the Th1 promoting cytokine IL-12 in dendritic cells as well as enhanced expression of the co-stimulatory molecules CD40, CD80, and CD86. In vivo studies showed that L. mexicana-infected mice treated by topical application of PARE resulted in the significant reduction in lesion size and parasite burden compared to controls. These findings indicate that PARE could be used as an alternative therapy for the topical treatment of CL.
authors.affiliation: Department of Pathology, Wexner Medical Center, The Ohio State University, Columbus, OH, 43210, USA.
authors.forename: Claudio M
authors.initials: CM
authors.lastname: Lezama-Dávila
authors.forename: Li
authors.initials: L
authors.lastname: Pan
authors.forename: Angelica P
authors.initials: AP
authors.lastname: Isaac-Márquez
authors.forename: Cesar
authors.initials: C
authors.lastname: Terrazas
authors.forename: Steve
authors.initials: S
authors.lastname: Oghumu
authors.forename: Ricardo
authors.initials: R
authors.lastname: Isaac-Márquez
authors.forename: M Y
authors.initials: MY
authors.lastname: Pech-Dzib
authors.forename: Joseph
authors.initials: J
authors.lastname: Barbi
authors.forename: Edward
authors.initials: E
authors.lastname: Calomeni
authors.forename: Narasimham
authors.initials: N
authors.lastname: Parinandi
authors.forename: A Douglas
authors.initials: AD
authors.lastname: Kinghorn
authors.forename: Abhay R
authors.initials: AR
authors.lastname: Satoskar
mesh_terms: D000818:Animals; D000977:Antiparasitic Agents / Q000494:pharmacology*; D029062:Apocynaceae / Q000737:chemistry*; D003713:Dendritic Cells / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D007894:Leishmania mexicana / Q000187:drug effects*; D016773:Leishmaniasis, Cutaneous / Q000188:drug therapy* / Q000469:parasitology; D008264:Macrophages / Q000187:drug effects / Q000469:parasitology; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D052002:NF-kappa B p50 Subunit / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000977:Antiparasitic Agents; D052002:NF-kappa B p50 Subunit; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; C073701:Nfkb1 protein, mouse; D018664:Interleukin-12; D007371:Interferon-gamma
keywords: Leishmania mexicana; Pentalinon andreuxii roots; cutaneous leishmaniasis; phytomedicinal agent