title: Watching single proteins using engineered nanopores.
abstract: Recent studies in the area of single-molecule detection of proteins with nanopores show a great promise in fundamental science, bionanotechnology and proteomics. In this mini-review, I discuss a comprehensive array of examinations of protein detection and characterization using protein and solid-state nanopores. These investigations demonstrate the power of the single-molecule nanopore measurements to reveal a broad range of functional, structural, biochemical and biophysical features of proteins, such as their backbone flexibility, enzymatic activity, binding affinity as well as their concentration, size and folding state. Engineered nanopores in organic materials and in inorganic membranes coupled with surface modification and protein engineering might provide a new generation of sensing devices for molecular biomedical diagnostics.
authors.affiliation: Department of Physics, Syracuse University, 201 Physics Building, Syracuse, New York 13244-1130, USA. lmovilea@physics.syr.edu.
authors.forename: Liviu
authors.initials: L
authors.lastname: Movileanu
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D006801:Humans; D008958:Models, Molecular; D058608:Nanopores / Q000648:ultrastructure*; D017510:Protein Folding; D021381:Protein Transport; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D011506:Proteins