title: Common developmental pathway for primitive erythrocytes and multipotent hematopoietic progenitors in early mouse development.
abstract: Development of the hematopoietic system proceeds in a multistep manner. Primitive erythrocytes are the first hematopoietic cells to be observed that were produced transiently in developing embryos. Multilineage lymphohematopoiesis occurs after the primitive erythropoiesis. However, the lineage relationship of cells that comprise embryonic hematopoietic system is not well characterized. To clarify this process, careful analyses of the embryonic cells that differentiate into these cell lineages are necessary. We identified the common precursors of primitive erythrocytes and multipotent hematopoietic cells in mouse embryonic stem cell cultures and mouse embryos. A subset defined as CD45(-)CD41(+)AA4.1(-) cells showed bipotential capability to produce primitive erythrocytes and lymphomyeloid cells at the single-cell level. The cell population was present in vivo before hematopoietic stem cells (HSCs) appeared. Our results show that primitive erythrocytes and lymphomyeloid cells are not completely separate cell lineages, and these precursors comprise the embryonic hematopoietic system before HSC emergence.
authors.affiliation: Department of Stem Cell and Developmental Biology, Mie University Graduate School of Medicine, Tsu 514-8507, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Yamane
authors.affiliation: Department of Stem Cell and Developmental Biology, Mie University Graduate School of Medicine, Tsu 514-8507, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Washino
authors.affiliation: Department of Stem Cell and Developmental Biology, Mie University Graduate School of Medicine, Tsu 514-8507, Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Yamazaki
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D019070:Cell Lineage / Q000235:genetics; D002469:Cell Separation; D047108:Embryonic Development / Q000235:genetics; D004912:Erythrocytes / Q000166:cytology*; D018507:Gene Expression Regulation, Developmental; D006412:Hematopoietic Stem Cells / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D039902:Multipotent Stem Cells