title: An actin-like protein is involved in regulation of mitochondrial and flagellar functions as well as in intramacrophage survival of Leishmania donovani.
abstract: Actin-related proteins are ubiquitous actin-like proteins that show high similarity with actin in terms of their amino acid sequence and three-dimensional structure. However, in lower eukaryotes, such as trypanosomatids, their functions have not yet been explored. Here, we show that a novel actin-related protein (ORF LmjF.13.0950) is localized mainly in the Leishmania mitochondrion. We further reveal that depletion of the intracellular levels of this protein leads to an appreciable decrease in the mitochondrial membrane potential as well as in the ATP production, which appears to be accompanied with impairment in the flagellum assembly and motility. Additionally, we report that the mutants so generated fail to survive inside the mouse peritoneal macrophages. These abnormalities are, however, reversed by the episomal gene complementation. Our results, for the first time indicate that apart from their classical roles in the cytoplasm and nucleus, actin-related proteins may also regulate the mitochondrial function, and in case of Leishmania donovani they may also serve as the essential factor for their survival in the host cells.
authors.affiliation: Molecular and Structural Biology Division, CSIR-Central Drug Research Institute, Lucknow, PIN-226031, Uttar Pradesh, India.
authors.forename: Kuldeep
authors.initials: K
authors.lastname: Singh
authors.forename: Niranjan K
authors.initials: NK
authors.lastname: Veluru
authors.forename: Vishal
authors.initials: V
authors.lastname: Trivedi
authors.forename: Chhitar M
authors.initials: CM
authors.lastname: Gupta
authors.forename: Amogh A
authors.initials: AA
authors.lastname: Sahasrabuddhe
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D005407:Flagella / Q000502:physiology*; D017353:Gene Deletion; D005816:Genetic Complementation Test; D007893:Leishmania donovani / Q000235:genetics / Q000276:immunology* / Q000502:physiology*; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000469:parasitology; D053078:Membrane Potential, Mitochondrial; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000199:Actins; D015800:Protozoan Proteins; D000255:Adenosine Triphosphate