title: Sterol-dependent induction of plant defense responses by a microbe-associated molecular pattern from Trichoderma viride.
abstract: Plant-microbe interactions involve numerous regulatory systems essential for plant defense against pathogens. An ethylene-inducing xylanase (Eix) of Trichoderma viride is a potent elicitor of plant defense responses in specific cultivars of tobacco (Nicotiana tabacum) and tomato (Solanum lycopersicum). We demonstrate that tomato cyclopropyl isomerase (SlCPI), an enzyme involved in sterol biosynthesis, interacts with the LeEix2 receptor. Moreover, we examined the role of SlCPI in signaling during the LeEix/Eix defense response. We found that SlCPI is an important factor in the regulation of the induction of defense responses such as the hypersensitive response, ethylene biosynthesis, and the induction of pathogenesis-related protein expression in the case of LeEix/Eix. Our results also suggest that changes in the sterol composition reduce LeEix internalization, thereby attenuating the induction of plant defense responses.
authors.affiliation: Department of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv, Israel 69978.
authors.forename: Miya
authors.initials: M
authors.lastname: Sharfman
authors.forename: Maya
authors.initials: M
authors.lastname: Bar
authors.forename: Silvia
authors.initials: S
authors.lastname: Schuster
authors.forename: Meirav
authors.initials: M
authors.lastname: Leibman
authors.forename: Adi
authors.initials: A
authors.lastname: Avni
mesh_terms: D004705:Endocytosis / Q000187:drug effects; D011992:Endosomes / Q000187:drug effects / Q000378:metabolism; D005030:Ethylenes / Q000096:biosynthesis; D005372:Filipin / Q000494:pharmacology; D020868:Gene Silencing / Q000187:drug effects; D019753:Intramolecular Lyases / Q000378:metabolism; D018551:Solanum lycopersicum / Q000276:immunology* / Q000382:microbiology*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D051192:Receptors, Pattern Recognition / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013261:Sterols / Q000378:metabolism*; D014026:Nicotiana / Q000276:immunology* / Q000382:microbiology*; D014242:Trichoderma / Q000187:drug effects / Q000502:physiology*; D020798:Two-Hybrid System Techniques
chemical_list: D005030:Ethylenes; D010940:Plant Proteins; D051192:Receptors, Pattern Recognition; D013261:Sterols; D005372:Filipin; C036216:ethylene; D019753:Intramolecular Lyases