title: The effect of 5'-adenylic acid on hepatic proteome of mice radiated by 60Co γ-ray.
abstract: Understanding the protection mechanism of 5'-AMP requires comprehensive knowledge of the proteins expressed during the period that the body is exposed to irradiation. Proteomics provides the tools for such analyses. Here, the experimental ICR mice were divided into three groups (normal group, model group and 5'-AMP + irradiation group). After different treatment, the hepatic total protein of each animal in three groups was separated by two-dimensional gel electrophoresis (2-DE). 2-DE analysis revealed fifty-eight protein spots were differentially expressed in comparison to three groups. From 58 protein spots, we selected nine spots to identify by MALDI-TOF-MS and received credible results. They were determined to be type I arginase, annexin A5, regucalcin, catalase, Tpm3 protein, Pdia4 protein, 14-3-3 protein epsilon, NAD-Malate dehydrogenase and heat shock protein 90. Considering the characteristic of these proteins, we proposed a possible protection pathway.
authors.forename: Cuilin
authors.initials: C
authors.lastname: Cheng
authors.forename: Haitian
authors.initials: H
authors.lastname: Zhao
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China. wzy219001@hit.edu.cn.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
authors.forename: Weihong
authors.initials: W
authors.lastname: Lu
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.forename: Rongchun
authors.initials: R
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Yao
mesh_terms: D000249:Adenosine Monophosphate / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002374:Catalase / Q000378:metabolism; D003037:Cobalt Radioisotopes / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D005720:Gamma Rays; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018832:Molecular Chaperones / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D003037:Cobalt Radioisotopes; D018832:Molecular Chaperones; D020543:Proteome; D000249:Adenosine Monophosphate; D002374:Catalase