title: Biomolecular analysis and biological tissue diagnostics by electrospray ionization with a metal wire inserted gel-loading tip.
abstract: A metal wire-inserted disposable gel-loading tip was examined as an electrospray emitter. Its performance was similar to that of conventional electrospray ionization (ESI) with a relatively low flow rate (~100 nL/min) and without the need for solvent pumps. It was also used as an emitter for solid probe-assisted ESI (SPA-ESI) (e.g., biofluid was sampled from the biological tissue by a needle and was inserted into the solvent-preloaded gel-loading tip). Selective detection of lipids and proteins, such α and β chains of hemoglobin could be accomplished by choosing appropriate solvents. A suitable protocol for cancer diagnosis was established by this method. A good figure of merit of this method is its applicability to biological tissue diagnostics with high cost efficiency and on a disposable basis.
authors.affiliation: Clean Energy Research Center, University of Yamanashi , 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan.
authors.forename: Mridul Kanti
authors.initials: MK
authors.lastname: Mandal
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yoshimura
authors.forename: Subhrakanti
authors.initials: S
authors.lastname: Saha
authors.forename: Zhan
authors.initials: Z
authors.lastname: Yu
authors.forename: Sen
authors.initials: S
authors.lastname: Takeda
authors.forename: Kenzo
authors.initials: K
authors.lastname: Hiraoka
mesh_terms: D002292:Carcinoma, Renal Cell / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology; D045304:Cytochromes c / Q000032:analysis; D004867:Equipment Design; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007328:Insulin / Q000032:analysis; D007668:Kidney / Q000737:chemistry* / Q000473:pathology; D007680:Kidney Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology; D009339:Needles; D010446:Peptide Fragments / Q000032:analysis; D025341:Principal Component Analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation; D025801:Ubiquitin / Q000032:analysis; D055544:beta-Globins / Q000032:analysis
chemical_list: D006454:Hemoglobins; D007328:Insulin; D010446:Peptide Fragments; D025801:Ubiquitin; D055544:beta-Globins; C070077:alpha(A) globin; D045304:Cytochromes c