title: Neuroprotective activity of gossypin from Hibiscus vitifolius against global cerebral ischemia model in rats.
abstract: OBJECTIVE
  The objective of this study is to evaluate the neuroprotective effect of gossypin (isolated from Hibiscus vitifolius) against global cerebral ischemia/reperfusion (I/R) injury-induced oxidative stress in rats.
  
  
  METHODS
  Sprague Dawlet rats of wither gender were used in the study. Evaluation of cerbroprotective activity of bioflavonoid gossypin (in 5, 10 and 20 mg/kg oral doses) isolated from H. vitifolius was carried out by using the global cerebral I/R model by bilateral carotid artery occlusion for 30 min, followed by 24 h reperfusion. The antioxidant enzymatic and non-enzymatic levels were estimated along with histopathological studies.
  
  
  RESULTS
  Gossypin showed dose-dependent neuroprotective activity by significant decrease in lipid peroxidation (P < 0.001) and increase in the superoxide dismutase, catalase, glutathione and total thiol levels in gossypin treated groups when compared to control group. Cerebral infarction area was markedly reduced in gossypin treated groups when compared to control group.
  
  
  CONCLUSIONS
  Gossypin showed potent neuroprotective activity against global cerebral I/R injury-induced oxidative stress in rats.
authors.affiliation: Department of Pharmacology, Hanagal Shri Kumareshwar College of Pharmacy, Bagalkot, Karnataka, India.
authors.forename: V M
authors.initials: VM
authors.lastname: Chandrashekhar
authors.affiliation: Pharmacognosy and Phytochemistry Division, College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, India.
authors.forename: S
authors.initials: S
authors.lastname: Ganapaty
authors.affiliation: CDSCO, Subzonal Office, Ahmedabad, Gujarat, India.
authors.forename: A
authors.initials: A
authors.lastname: Ramkishan
authors.affiliation: Center of Biotechnology, Institute of Science and Technology, JNT University, Hyderabad, Andhra Pradesh, India.
authors.forename: M Laxmi
authors.initials: ML
authors.lastname: Narsu
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000517:prevention & control*; D002374:Catalase / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D031584:Hibiscus / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D005419:Flavonoids; D018696:Neuroprotective Agents; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; C022944:gossypin; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Cerebroprotective; flavonoids; gossypin; ischemia/reperfusion; natural products; oxidative stress