title: LC-MS/MS in endocrinology: what is the profit of the last 5 years?
abstract: Currently, chromatography (GC but more commonly HPLC) is the analytical method of choice for several hormones, either because the immunoassays suffer from extensive crossreactivity or because chromatography permits simultaneous measurements of hormones. However, sometimes the conventional detection systems with HPLC methods do not meet desired specificity. With the increase of robust and affordable LC-MS/MS systems, the next step forward in specificity was taken. LC-MS/MS is rapidly being incorporated in the endocrine laboratories. To be useful in the clinical diagnostic practice, it is of utmost importance that methods are both analytically and clinically vaidated, as until now, the majority of applications of LC-MS/MS in the clinical laboratories are 'home-made' methods, therefore special case must be taken. This review aims to focus on Clinical and Laboratory Standards Institute or comparable validated LC-MS/MS methods for targeted hormone analysis used for diagnostic purposes in human samples, published in the last 5 years.
authors.affiliation: Department of Clinical Chemistry, Laboratory of Endocrinology, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands.
authors.forename: Mariëtte Theodora
authors.initials: MT
authors.lastname: Ackermans
authors.forename: Erik
authors.initials: E
authors.lastname: Endert
mesh_terms: D001679:Biogenic Amines / Q000097:blood / Q000652:urine; D002853:Chromatography, Liquid / Q000592:standards*; D004704:Endocrinology / Q000379:methods / Q000592:standards*; D006197:Hair / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D010281:Parathyroid Hormone / Q000097:blood / Q000652:urine; D012463:Saliva / Q000737:chemistry; D013256:Steroids / Q000097:blood / Q000652:urine; D053719:Tandem Mass Spectrometry / Q000592:standards*; D013972:Thyrotropin / Q000097:blood / Q000652:urine; D054928:Validation Studies as Topic; D014807:Vitamin D / Q000097:blood / Q000652:urine
chemical_list: D001679:Biogenic Amines; D010281:Parathyroid Hormone; D013256:Steroids; D014807:Vitamin D; D013972:Thyrotropin