title: ChIP-seq analysis of histone H3K9 trimethylation in peripheral blood mononuclear cells of membranous nephropathy patients.
abstract: Membranous nephropathy (MN), characterized by the presence of diffuse thickening of the glomerular basement membrane and subepithelial in situ immune complex disposition, is the most common cause of idiopathic nephrotic syndrome in adults, with an incidence of 5-10 per million per year. A number of studies have confirmed the relevance of several experimental insights to the pathogenesis of human MN, but the specific biomarkers of MN have not been fully elucidated. As a result, our knowledge of the alterations in histone methylation in MN is unclear. We used chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) to analyze the variations in a methylated histone (H3K9me3) in peripheral blood mononuclear cells from 10 MN patients and 10 healthy subjects. There were 108 genes with significantly different expression in the MN patients compared with the normal controls. In MN patients, significantly increased activity was seen in 75 H3K9me3 genes, and decreased activity was seen in 33, compared with healthy subjects. Five positive genes, DiGeorge syndrome critical region gene 6 (DGCR6), sorting nexin 16 (SNX16), contactin 4 (CNTN4), baculoviral IAP repeat containing 3 (BIRC3), and baculoviral IAP repeat containing 2 (BIRC2), were selected and quantified. There were alterations of H3K9me3 in MN patients. These may be candidates to help explain pathogenesis in MN patients. Such novel findings show that H3K9me3 may be a potential biomarker or promising target for epigenetic-based MN therapies.
authors.affiliation: 181st Hospital, Nephrology Department, Guangxi Key Laboratory of Metabolic Diseases Research, GuilinGuangxi, China, Guangxi Key Laboratory of Metabolic Diseases Research, Nephrology Department, 181st Hospital, Guilin, Guangxi, China.
authors.forename: W G
authors.initials: WG
authors.lastname: Sui
authors.affiliation: Guangxi Normal University, The Life Science College, GuilinGuangxi, China, The Life Science College, Guangxi Normal University, Guilin, Guangxi, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: He
authors.affiliation: 181st Hospital, Nephrology Department, Guangxi Key Laboratory of Metabolic Diseases Research, GuilinGuangxi, China, Guangxi Key Laboratory of Metabolic Diseases Research, Nephrology Department, 181st Hospital, Guilin, Guangxi, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Yan
authors.affiliation: 181st Hospital, Nephrology Department, Guangxi Key Laboratory of Metabolic Diseases Research, GuilinGuangxi, China, Guangxi Key Laboratory of Metabolic Diseases Research, Nephrology Department, 181st Hospital, Guilin, Guangxi, China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Chen
authors.affiliation: Guangxi Normal University, The Life Science College, GuilinGuangxi, China, The Life Science College, Guangxi Normal University, Guilin, Guangxi, China.
authors.forename: R H
authors.initials: RH
authors.lastname: Zhang
authors.affiliation: Jinan University, Shenzhen People's Hospital, The Second Clinical Medical College, Clinical Medical Research Center, ShenzhenGuangdong, China, Clinical Medical Research Center, The Second Clinical Medical College, Shenzhen People's Hospital, Jinan University, Shenzhen, Guangdong, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Dai
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D047369:Chromatin Immunoprecipitation; D005260:Female; D015433:Glomerulonephritis, Membranous / Q000235:genetics* / Q000378:metabolism; D006657:Histones / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008239:Lysine / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008745:Methylation
chemical_list: D006657:Histones; D008239:Lysine