title: Overexpression of small heat shock protein LimHSP16.45 in Arabidopsis enhances tolerance to abiotic stresses.
abstract: Small heat shock proteins (smHSPs) play important and extensive roles in plant defenses against abiotic stresses. We cloned a gene for a smHSP from the David Lily (Lilium davidii (E. H. Wilson) Raffill var. Willmottiae), which we named LimHSP16.45 based on its protein molecular weight. Its expression was induced by many kinds of abiotic stresses in both the lily and transgenic plants of Arabidopsis. Heterologous expression enhanced cell viability of the latter under high temperatures, high salt, and oxidative stress, and heat shock granules (HSGs) formed under heat or salinity treatment. Assays of enzymes showed that LimHSP16.45 overexpression was related to greater activity by superoxide dismutase and catalase in transgenic lines. Therefore, we conclude that heterologous expression can protect plants against abiotic stresses by preventing irreversible protein aggregation, and by scavenging cellular reactive oxygen species.
authors.affiliation: Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou, P.R. China.
authors.forename: Changjun
authors.initials: C
authors.lastname: Mu
authors.affiliation: Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou, P.R. China.
authors.forename: Shijia
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou, P.R. China.
authors.forename: Guanzhong
authors.initials: G
authors.lastname: Yu
authors.affiliation: Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou, P.R. China.
authors.forename: Ni
authors.initials: N
authors.lastname: Chen
authors.affiliation: Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou, P.R. China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou, P.R. China.
authors.forename: Heng
authors.initials: H
authors.lastname: Liu
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D002374:Catalase / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival / Q000235:genetics; D003594:Cytoplasmic Granules / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D027762:Lilium / Q000378:metabolism*; D018384:Oxidative Stress / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D054712:Salinity; D013312:Stress, Physiological / Q000235:genetics; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D006360:Heat-Shock Proteins; D010940:Plant Proteins; D049452:Green Fluorescent Proteins; D002374:Catalase; D013482:Superoxide Dismutase