title: Extraction optimization, isolation, preliminary structural characterization and antioxidant activities of the cell wall polysaccharides in the petioles and pedicels of Chinese herbal medicine Qian (Euryale ferox Salisb.).
abstract: Cell wall polysaccharides in the petioles and pedicels of Qian (Euryale ferox Salisb.) (EFPP) were extracted using ultrasound-assisted technique. Response surface methodology (RSM) based on Box-Behnken design (BBD) was employed to optimize extraction parameters for the maximum purity of polysaccharides. The results showed that the optimum extraction conditions were extraction temperature of 80 °C, extraction time of 32 min, ultrasonic power of 270W and liquid-to-solid ratio of 40 mL/g. Under the optimal conditions, the experimental purity of polysaccharides was 62.57% ± 1.68%, which was very close to the predicted. The crude EFPP were isolated using DEAE-52 column and four major fractions (EFPP-1, EFPP-2, EFPP-3 and EFPP-4) were obtained. Typical functional groups of polysaccharides were characteristic for EFPP-1, EFPP-3 and EFPP-4 from FT-IR spectrum. Furthermore, the crude EFPP and three fractions (EFPP-1, EFPP-3 and EFPP-4) possessed appreciable in vitro antioxidant effects on α,α-diphenyl-β-picrylhydrazyl (DPPH), 2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), hydroxyl radical scavenging and reducing powers. Then, the crude EFPP and EFPP-4 could effective against H2O2-induced injury on HUVEC and VSMC through enhancement of T-AOC, SOD and CAT activities and decrease of MDA content.
authors.affiliation: The School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China.
authors.forename: Chengying
authors.initials: C
authors.lastname: Wu
authors.affiliation: The School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China; Chemical and Pharmaceutical School, Henan University of Science and Technology, Luoyang 471003, Henan, China.
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Wang
authors.affiliation: The School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: The School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China; Hanlin College, Nanjing University of Chinese Medicine, Taizhou 225300, Jiangsu, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Shen
authors.affiliation: The School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: He
authors.affiliation: The School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
authors.affiliation: The School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China; Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing 210023, Jiangsu, China; Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China. Electronic address: qnwyjs@163.com.
authors.forename: Qinan
authors.initials: Q
authors.lastname: Wu
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D002473:Cell Wall / Q000737:chemistry*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D064028:Tracheophyta / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D011134:Polysaccharides
keywords: Antioxidant activities; Euryale ferox Salisb.; Petioles and pedicels; Polysaccharides; Ultrasound-assisted extraction