title: pH-rate profiles of L-arabinitol 4-dehydrogenase from Hypocrea jecorina and its application in L-xylulose production.
abstract: l-Arabinitol 4-dehydrogenase (LAD) from Hypocrea jecorina (HjLAD) was cloned and overexpressed in Escherichia coli BL21 (DE3). The kinetics of l-arabinitol oxidation by NAD(+), catalyzed by HjLAD, was studied within the pH range of 7.0-9.5 at 25°C. The turnover number (kcat) and the catalytic efficiency (kcat/Km) were 4200min(-1) and 290mM(-1)min(-1), respectively. HjLAD showed the highest turnover number and catalytic efficiency among all previously characterized LADs. In further application of HjLAD, rare l-sugar l-xylulose was produced by the enzymatic oxidation of arabinitol to give a yield of approximately 86%.
authors.affiliation: Department of Chemical Engineering, Konkuk University, Seoul 143-701, Republic of Korea; Institute of SK-KU Biomaterials, Konkuk University, Seoul 143-701, Republic of Korea; Department of Environmental Engineering, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Manish Kumar
authors.initials: MK
authors.lastname: Tiwari
authors.affiliation: Department of Chemical Engineering, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Raushan Kumar
authors.initials: RK
authors.lastname: Singh
authors.affiliation: Department of Chemical Engineering, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Hui
authors.initials: H
authors.lastname: Gao
authors.affiliation: Department of Chemical Engineering, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Taesu
authors.initials: T
authors.lastname: Kim
authors.affiliation: Department of Biomedical Sciences, University of Ulsan, College of Medicine, Seoul 138-736, Republic of Korea.
authors.forename: Suhwan
authors.initials: S
authors.lastname: Chang
authors.affiliation: Institute of SK-KU Biomaterials, Konkuk University, Seoul 143-701, Republic of Korea; Department of Environmental Engineering, Konkuk University, Seoul 143-701, Republic of Korea. Electronic address: hankim@konkuk.ac.kr.
authors.forename: Han S
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Chemical Engineering, Konkuk University, Seoul 143-701, Republic of Korea; Institute of SK-KU Biomaterials, Konkuk University, Seoul 143-701, Republic of Korea. Electronic address: jkrhee@konkuk.ac.kr.
authors.forename: Jung-Kul
authors.initials: JK
authors.lastname: Lee
mesh_terms: D055162:Biocatalysis; D006863:Hydrogen-Ion Concentration; D035901:Hypocrea / Q000201:enzymology*; D007700:Kinetics; D008958:Models, Molecular; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D013401:Sugar Alcohol Dehydrogenases / Q000302:isolation & purification / Q000378:metabolism*; D014996:Xylulose / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D011994:Recombinant Proteins; D014996:Xylulose; D013401:Sugar Alcohol Dehydrogenases; C440029:L-arabinitol 4-dehydrogenase
keywords: Molecular dynamics; Rare sugar; l-Xylulose; pH-Dependent kinetics