title: Genetic Analysis of cagA and vacA Genes in Helicobacter Pylori Isolates and Their Relationship with Gastroduodenal Diseases in the West of Iran.
abstract: BACKGROUND
  Helicobacter pylori have different virulence factors which are associated with several gastroduodenal diseases; however, this association is variable in different geographical regions. Data of genotypes of Iranian H. pylori isolates are few.
  
  
  OBJECTIVE
  The aim of the current study was to investigate the cagA/vacA genotypes of Helicobacter pylori isolates and determine the relationship between these genotypes with respect to different gastric disorders in patients of Chaharmahalo Bakhtiarian.
  
  
  METHODS
  In this cross-sectional study, gastric biopsies were taken from 200 patients with gastrodoudenal diseases. Histopathological features were recognized by specialist. The samples were subjected to PCR for detection and identification of ureC, cagA and vacA genes.
  
  
  RESULTS
  The frequency of the vacA genotypes, sa1/m1, s1a/m1b, s1a/m2, s1b/m1a, s1b/m1b, s1b/m2, s1c/m1a, s1c/m1b, s1c/m2, s2/m1a, s2/m1b and s2/m2 were 27(6.6%), 8(4.3%), 45(28.04%), 7(3.7%), 5(2.5%), 10 (6.1%), 12 (7.4%), 4 (2.5%), 18(11%), 6(3.7%), 0 and 22(13.5%) respectively. The cagA gene was detected in 92% of strains. Based on our findings, it seemed that cagPAI and vacA s1 genotypes were associated with some gastric disorders in patients with H. pylori. In this region, the isolates carrying s1a/m2 were the most prevalent.
  
  
  CONCLUSIONS
  We found considerable relationship between s1a/m1a, s1a/m2, s2/m2 and s1c/m1a and some gastric disorders. Further studies about the role of H. pylori virulence factors and gastric disorders were recommended.
authors.affiliation: Young Researchers and Elite Club, Jahrom Branch, Islamic Azad University, Jahrom, IR Iran.
authors.forename: Negar
authors.initials: N
authors.lastname: Souod
authors.affiliation: Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, IR Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Kargar
authors.affiliation: Department of Biotechnology, Shahrekord Branch, Islamic Azad University, Shahrekord, IR Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Doosti
authors.affiliation: Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Ranjbar
authors.affiliation: Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.
authors.forename: Meysam
authors.initials: M
authors.lastname: Sarshar
keywords: CagA protein, Helicobacter Pylori; Helicobacter Pylori; VacA Protein, Helicobacter Pylori