title: Coordinated transcriptional regulation of isopentenyl diphosphate biosynthetic pathway enzymes in plastids by phytochrome-interacting factor 5.
abstract: All isoprenoids are derived from a common C5 unit, isopentenyl diphosphate (IPP). In plants, IPP is synthesized via two distinct pathways; the cytosolic mevalonate pathway and the plastidial non-mevalonate (MEP) pathway. In this study, we used a co-expression analysis to identify transcription factors that coordinately regulate the expression of multiple genes encoding enzymes in the IPP biosynthetic pathway. Some candidates showed especially strong correlations with multiple genes encoding MEP-pathway enzymes. We report here that phytochrome-interacting factor 5 (PIF5), a basic-helix-loop-helix type transcription factor, functions as a positive regulator of the MEP pathway. Its overexpression in T87 suspension cultured cells resulted in increased accumulation of chlorophylls and carotenoids. Detailed analyses of carotenoids by HPLC indicated that some carotenoid biosynthetic pathways were concomitantly up-regulated, possibly as a result of enhanced IPP metabolic flow. Our results also revealed other PIF family proteins that play different roles from that of PIF5 in IPP metabolism.
authors.affiliation: Graduate School of Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan.
authors.forename: Kazuto
authors.initials: K
authors.lastname: Mannen
authors.affiliation: Graduate School of Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan.
authors.forename: Takuro
authors.initials: T
authors.lastname: Matsumoto
authors.affiliation: Graduate School of Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan. Electronic address: takahasi@seika.che.tohoku.ac.jp.
authors.forename: Seiji
authors.initials: S
authors.lastname: Takahashi
authors.affiliation: Graduate School of Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Yamaguchi
authors.affiliation: Graduate School of Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Tsukagoshi
authors.affiliation: Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Sano
authors.affiliation: Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Suzuki
authors.affiliation: Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Nozomu
authors.initials: N
authors.lastname: Sakurai
authors.affiliation: Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Shibata
authors.affiliation: Institute of Multidisciplinary Research for Advanced Material, Tohoku University, Sendai, Miyagi 980-8577, Japan.
authors.forename: Tanetoshi
authors.initials: T
authors.lastname: Koyama
authors.affiliation: Graduate School of Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Nakayama
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics*; D045782:Hemiterpenes / Q000096:biosynthesis* / Q000235:genetics; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism; D009943:Organophosphorus Compounds; D018087:Plastids / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D045782:Hemiterpenes; D009097:Multienzyme Complexes; D009943:Organophosphorus Compounds; D014157:Transcription Factors; C497729:PIF5 protein, Arabidopsis; C004809:isopentenyl pyrophosphate
keywords: 1-deoxy-d-xylulose 5-phosphate; 1-hydroxy-2-methyl-2-butenyl 4-diphosphate; Arabidopsis thaliana; Co-expression analysis; DXP; GGR; HMBPP; IPP; Isoprenoids; MEP; PDS; PIF5; PIFs; Phytochrome-interacting factor; bHLH; basic helix-loop-helix; geranylgeranyl reductase; isopentyl diphosphate; methyl-d-erythritol 5-phosphate; phytochrome-interacting factor 5; phytochrome-interacting factors; phytoene desaturase