title: Genome sequencing and molecular characterisation of Staphylococcus aureus ST772-MRSA-V, "Bengal Bay Clone".
abstract: BACKGROUND
  The PVL-positive ST772-MRSA-V is an emerging community-associated (CA-) MRSA clone that has been named Bengal Bay Clone since most patients have epidemiological connections to the Indian subcontinent. It is found increasingly common in other areas of the world.
  
  
  METHODS
  One isolate of ST772-MRSA-V was sequenced using the Illumina Genome Analyzer System. After initial assembling the multiple sequence contigs were analysed using different in-house annotation scripts. Results were compared to microarray hybridisation results of clinical isolates of ST772-MRSA-V, of related strains and to another ST772-MRSA-V genome sequence.
  
  
  RESULTS
  According to MLST e-burst analysis, ST772-MRSA-V belongs to Clonal Complex (CC)1, differing from ST1 only in one MLST allele (pta-22). However, there are several additional differences including agr alleles (group II rather than III), capsule type (5 rather than 8), the presence of the egc enterotoxin gene cluster and of the enterotoxin homologue ORF CM14 as well as the absence of the enterotoxin H gene seh. Enterotoxin genes sec and sel are present. ST772-MRSA-V harbours the genes encoding enterotoxin A (sea) and PVL (lukS/F-PV). Both are located on the same prophage.
  
  
  CONCLUSIONS
  ST772-MRSA-V may have emerged from the same lineage as globally spread CC1 and CC5 strains. It has acquired a variety of virulence factors, and for a CA-MRSA strain it has an unusually high number of genes associated with antibiotic resistance.
authors.affiliation: Institute for Medical Microbiology and Hygiene, Technical University of Dresden, Dresden, Germany. monecke@rocketmail.com.
authors.forename: Stefan
authors.initials: S
authors.lastname: Monecke
authors.forename: Vico
authors.initials: V
authors.lastname: Baier
authors.forename: Geoffrey W
authors.initials: GW
authors.lastname: Coombs
authors.forename: Peter
authors.initials: P
authors.lastname: Slickers
authors.forename: Albrecht
authors.initials: A
authors.lastname: Ziegler
authors.forename: Ralf
authors.initials: R
authors.lastname: Ehricht
mesh_terms: D000483:Alleles; D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics*; D002999:Clone Cells; D016680:Genome, Bacterial; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D007194:India; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D058977:Molecular Sequence Annotation; D058885:Multilocus Sequence Typing; D058974:Phylogeography; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins