title: LC-MS guided isolation of (±)-sweriledugenin A, a pair of enantiomeric lactones, from Swertia leducii.
abstract: (±)-Sweriledugenin A, a pair of novel enantiomeric lactones, were isolated from Swertia leducii under the guidance of LC-MS investigation. The enantiomeric separation was achieved by HPLC on a chiral column. Their structures were determined by extensive NMR spectra, X-ray, and quantum calculations. (+)-Sweriledugenin A and (-)-sweriledugenin A showed activities inhibiting HBV DNA replication with the IC50 values of 36.86 and 26.55 μM on the HepG 2.2.15 cell line in vitro.
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany , Chinese Academy of Sciences, Kunming 650201, P.R.China.
authors.forename: Chang-An
authors.initials: CA
authors.lastname: Geng
authors.forename: Xing-Long
authors.initials: XL
authors.lastname: Chen
authors.forename: Ning-Jia
authors.initials: NJ
authors.lastname: Zhou
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Yun-Bao
authors.initials: YB
authors.lastname: Ma
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Huang
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Zhang
authors.forename: Ji-Jun
authors.initials: JJ
authors.lastname: Chen
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D056945:Hep G2 Cells; D006515:Hepatitis B virus / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D013237:Stereoisomerism; D029967:Swertia / Q000737:chemistry*
chemical_list: D000998:Antiviral Agents; D007783:Lactones; C000589873:sweriledugenin A