title: A continuous-flow high-throughput microfluidic device for airborne bacteria PCR detection.
abstract: Rapid analysis of airborne pathogens plays a critical role in early warning of spreading infectious diseases, which is essential for public health and disease prevention. Herein we report an integrated microfluidic device that can perform airborne pathogen capture, enrichment and continuous-flow high-throughput gene analysis. The device was validated by six frequently encountered bacteria and shows great potential in environmental analysis and for public health protection.
authors.affiliation: Shanghai Key laboratory of Atmospheric Particle Pollution Prevention (LAP3), Department of Environmental Science & Engineering, Institute of Biomedical Science, Fudan University, Shanghai 200433, PR China. gsui@fudan.edu.cn.
authors.forename: Xiran
authors.initials: X
authors.lastname: Jiang
authors.forename: Wenwen
authors.initials: W
authors.lastname: Jing
authors.forename: Lulu
authors.initials: L
authors.lastname: Zheng
authors.forename: Sixiu
authors.initials: S
authors.lastname: Liu
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Wu
authors.forename: Guodong
authors.initials: G
authors.lastname: Sui
mesh_terms: D000391:Air Microbiology; D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*