title: Protein kinase LKB1 regulates polarized dendrite formation of adult hippocampal newborn neurons.
abstract: Adult-born granule cells in the dentate gyrus of the rodent hippocampus are important for memory formation and mood regulation, but the cellular mechanism underlying their polarized development, a process critical for their incorporation into functional circuits, remains unknown. We found that deletion of the serine-threonine protein kinase LKB1 or overexpression of dominant-negative LKB1 reduced the polarized initiation of the primary dendrite from the soma and disrupted its oriented growth toward the molecular layer. This abnormality correlated with the dispersion of Golgi apparatus that normally accumulated at the base and within the initial segment of the primary dendrite, and was mimicked by disrupting Golgi organization via altering the expression of Golgi structural proteins GM130 or GRASP65. Thus, besides its known function in axon formation in embryonic pyramidal neurons, LKB1 plays an additional role in regulating polarized dendrite morphogenesis in adult-born granule cells in the hippocampus.
authors.affiliation: Institute of Neuroscience, State Key Laboratory of Neuroscience, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China 200031.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Liang
authors.initials: L
authors.lastname: She
authors.forename: Xing-ya
authors.initials: XY
authors.lastname: Chang
authors.forename: Rong-rong
authors.initials: RR
authors.lastname: Yang
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Hong-bin
authors.initials: HB
authors.lastname: Ji
authors.forename: Jian-wei
authors.initials: JW
authors.lastname: Jiao
authors.forename: Mu-ming
authors.initials: MM
authors.lastname: Poo
mesh_terms: D055372:AMP-Activated Protein Kinases; D000818:Animals; D000831:Animals, Newborn; D001324:Autoantigens / Q000378:metabolism; D001369:Axons / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D016764:Cell Polarity; D049109:Cell Proliferation; D003712:Dendrites / Q000378:metabolism*; D018891:Dentate Gyrus / Q000378:metabolism*; D005786:Gene Expression Regulation; D006056:Golgi Apparatus / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018613:Microscopy, Confocal; D055495:Neurogenesis; D009474:Neurons / Q000378:metabolism*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*
chemical_list: D001324:Autoantigens; D002352:Carrier Proteins; C097741:Golgin subfamily A member 2; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C523888:tamalin protein, mouse; D049452:Green Fluorescent Proteins; D017346:Protein Serine-Threonine Kinases; C491654:Stk11 protein, mouse; D055372:AMP-Activated Protein Kinases
keywords: Golgi deployment; adult neurogenesis; neuronal polarization