title: The inhibition of lamellar hydroxyapatite and lamellar magnetic hydroxyapatite on the migration and adhesion of breast cancer cells.
abstract: Hydroxyapatite nanoparticles have been reported to exhibit potent anti-tumor effects in some cancer cells. In our previous study, we have successfully synthesized two types of hydroxyapatite nanoparticles, laminated hydroxyapatite (L-HAp) and laminated magnetic hydroxyapatite (LM-HAp). In this study, we wanted to investigate the effects of L-HAp and LM-HAp with various concentrations on human breast cancer MDA-MB-231 cells. Cell proliferation was assessed with a MTT colorimetric assay. Scratch and adhesion assays were used to detect the effects of these two materials on migration and adhesion. The expressions of integrin β1 and Akt were measured by Western blotting. Our results showed that L-HAp and LM-HAp had little cell cytotoxicity and significantly reduced cell mobility and adhesion. LM-HAp showed greater inhibitor ability on migration and adhesion of MDA-MB-231 cells. Moreover, results from western blotting showed that L-HAp and LM-HAp impacted the phosphorylation of integrin β1, but showed no regular impact on Akt. This study suggests that L-HAp and LM-HAp may be potential anti-tumor and delivery system for breast cancer therapy.
authors.affiliation: Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science and Engineering, Tianjin University, Tianjin, 300072, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Jin
authors.forename: Guifu
authors.initials: G
authors.lastname: Zuo
authors.forename: Guangyao
authors.initials: G
authors.lastname: Xiong
authors.forename: Honglin
authors.initials: H
authors.lastname: Luo
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Li
authors.forename: Chunying
authors.initials: C
authors.lastname: Ma
authors.forename: Deying
authors.initials: D
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Gu
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Ma
authors.forename: Yizao
authors.initials: Y
authors.lastname: Wan
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems; D017886:Durapatite / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles / Q000737:chemistry; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D058185:Magnetite Nanoparticles; D017886:Durapatite