title: Development of a novel immunochromatographic assay for rapid detection of VanA ligase-producing vancomycin-resistant enterococci.
abstract: We developed a novel immunochromatographic assay (ICA) (EZ-Step VanA rapid kit; Dinona, Korea) for the detection of VanA ligase from vancomycin-resistant enterococci (VRE). Of eight monoclonal antibodies screened by ELISAs, the VanA ligase ICA constructed with 1H9 plus 3G11 showed the greatest reactivity. The detection limit of the kit was 6.3 × 10(6) CFU per test. Of 127 vancomycin-resistant microorganisms, 100 vanA VRE were positive in the VanA ligase ICA, and 27 non-vanA vancomycin-resistant isolates were negative. These results were consistent with those of the PCR analyses. Thus, our ICA is a reliable and easy-to-use immunological assay for detecting VanA-producing VRE in clinical laboratories.
authors.affiliation: Department of Patholgoy, Chungbuk National University College of Medicine, Cheongju, 361-763, Republic of Korea, Dinoa Co, Iksan, 570-912, Republic of Korea.
authors.forename: Gil Yong
authors.initials: GY
authors.lastname: Ji
authors.forename: Hyung Geun
authors.initials: HG
authors.lastname: Song
authors.forename: Bo Ra
authors.initials: BR
authors.lastname: Son
authors.forename: Seung Bok
authors.initials: SB
authors.lastname: Hong
authors.forename: Jong Wan
authors.initials: JW
authors.lastname: Kim
authors.forename: Kyeong Seob
authors.initials: KS
authors.lastname: Shin
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D019729:Carbon-Oxygen Ligases / Q000032:analysis*; D002846:Chromatography, Affinity / Q000379:methods*; D016983:Enterococcus / Q000737:chemistry* / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests / Q000379:methods; D012680:Sensitivity and Specificity; D020713:Vancomycin Resistance
chemical_list: D001426:Bacterial Proteins; C071437:VanA ligase, Bacteria; D019729:Carbon-Oxygen Ligases