title: Biosensor based on nanocomposite material for pathogenic virus detection.
abstract: This paper introduces a DNA biosensor based on a DNA/chitosan/multi-walled carbon nanotube nanocomposite for pathogenic virus detection. An easy, cost-effective approach to the immobilization of probe DNA sequences on the sensor surface was performed. Cyclic voltammograms were used to characterize the probe DNA sequence immobilization. Complementary sequence hybridization was examined by electrochemical impedance spectroscopy. Results revealed that the developed DNA sensor can detect a target DNA concentration as low as 0.01×10(-12) M. The sensitivity of the prepared sensor was 52.57 kΩ/fM. The reusability and storage stability of the DNA sensor were also investigated. Results showed that the electron-transfer resistance decreased to approximately 35% after 8 weeks and to approximately 80% after 12 weeks of storage.
authors.affiliation: Advanced Institute for Science and Technology (AIST), Hanoi University of Science and Technology, Viet Nam; Faculty of Occupational Safety and Health (OSH), Trade Union University (TUU), Hanoi, Viet Nam.
authors.forename: Vu
authors.initials: V
authors.lastname: Van Thu
authors.affiliation: Advanced Institute for Science and Technology (AIST), Hanoi University of Science and Technology, Viet Nam.
authors.forename: Phuong Trung
authors.initials: PT
authors.lastname: Dung
authors.affiliation: Advanced Institute for Science and Technology (AIST), Hanoi University of Science and Technology, Viet Nam.
authors.forename: Le Thi
authors.initials: le T
authors.lastname: Tam
authors.affiliation: Advanced Institute for Science and Technology (AIST), Hanoi University of Science and Technology, Viet Nam. Electronic address: tam.phuongdinh@hust.edu.vn.
authors.forename: Phuong Dinh
authors.initials: PD
authors.lastname: Tam
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D003198:Computer Simulation; D004247:DNA / Q000032:analysis; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques; D004566:Electrodes; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D059027:Recycling; D014780:Viruses / Q000302:isolation & purification*
chemical_list: D037742:Nanotubes, Carbon; D004247:DNA
keywords: Biosensor; Carbon nanotube; DNA; DNA sensor; Functionalization