title: Increased pathogenicity of West Nile virus (WNV) by glycosylation of envelope protein and seroprevalence of WNV in wild birds in Far Eastern Russia.
abstract: In this review, we discuss the possibility that the glycosylation of West Nile (WN) virus E-protein may be associated with enhanced pathogenicity and higher replication of WN virus. The results indicate that E-protein glycosylation allows the virus to multiply in a heat-stable manner and therefore, has a critical role in enhanced viremic levels and virulence of WN virus in young-chick infection model. The effect of the glycosylation of the E protein on the pathogenicity of WN virus in young chicks was further investigated. The results indicate that glycosylation of the WN virus E protein is important for viral multiplication in peripheral organs and that it is associated with the strong pathogenicity of WN virus in birds. The micro-focus reduction neutralization test (FRNT) in which a large number of serum samples can be handled at once with a small volume (15 μL) of serum was useful for differential diagnosis between Japanese encephalitis and WN virus infections in infected chicks. Serological investigation was performed among wild birds in the Far Eastern region of Russia using the FRNT. Antibodies specific to WN virus were detected in 21 samples of resident and migratory birds out of 145 wild bird samples in the region.
authors.affiliation: Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-Ku, Sapporo 060-0818, Japan. kariwa@vetmed.hokudai.ac.jp.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kariwa
authors.forename: Ryo
authors.initials: R
authors.lastname: Murata
authors.forename: Masashi
authors.initials: M
authors.lastname: Totani
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yoshii
authors.forename: Ikuo
authors.initials: I
authors.lastname: Takashima
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000175:diagnosis / Q000453:epidemiology* / Q000821:virology; D001717:Birds; D006031:Glycosylation; D016036:Seroepidemiologic Studies; D012800:Siberia / Q000453:epidemiology; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014901:West Nile Fever / Q000175:diagnosis / Q000453:epidemiology* / Q000821:virology; D014902:West Nile virus / Q000302:isolation & purification / Q000472:pathogenicity* / Q000502:physiology
chemical_list: D014759:Viral Envelope Proteins