title: Experimental infection of macaques with a wild water bird-derived highly pathogenic avian influenza virus (H5N1).
abstract: Highly pathogenic avian influenza virus (HPAIV) continues to threaten human health. Non-human primate infection models of human influenza are desired. To establish an animal infection model with more natural transmission and to determine the pathogenicity of HPAIV isolated from a wild water bird in primates, we administered a Japanese isolate of HPAIV (A/whooper swan/Hokkaido/1/2008, H5N1 clade 2.3.2.1) to rhesus and cynomolgus monkeys, in droplet form, via the intratracheal route. Infection of the lower and upper respiratory tracts and viral shedding were observed in both macaques. Inoculation of rhesus monkeys with higher doses of the isolate resulted in stronger clinical symptoms of influenza. Our results demonstrate that HPAIV isolated from a water bird in Japan is pathogenic in monkeys by experimental inoculation, and provide a new method for HPAIV infection of non-human primate hosts, a good animal model for investigation of HPAIV pathogenicity.
authors.affiliation: Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Fujiyuki
authors.affiliation: Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
authors.forename: Misako
authors.initials: M
authors.lastname: Yoneda
authors.affiliation: Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Yasui
authors.affiliation: Amami Laboratory of Injurious Animals, The Institute of Medical Science, The University of Tokyo, Kagoshima, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kuraishi
authors.affiliation: Amami Laboratory of Injurious Animals, The Institute of Medical Science, The University of Tokyo, Kagoshima, Japan.
authors.forename: Shosaku
authors.initials: S
authors.lastname: Hattori
authors.affiliation: Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
authors.forename: Hyun-Jeong
authors.initials: HJ
authors.lastname: Kwon
authors.affiliation: Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Munekata
authors.affiliation: Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Kiso
authors.affiliation: Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kida
authors.affiliation: Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.
authors.forename: Michinori
authors.initials: M
authors.lastname: Kohara
authors.affiliation: Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
authors.forename: Chieko
authors.initials: C
authors.lastname: Kai
mesh_terms: D000818:Animals; D001717:Birds / Q000821:virology*; D004195:Disease Models, Animal; D053124:Influenza A Virus, H5N1 Subtype / Q000472:pathogenicity*; D008253:Macaca mulatta; D009976:Orthomyxoviridae Infections / Q000821:virology*