title: Concomitant caries and calculus formation from in situ dentin caries model.
abstract: The aim of this study was to test the possibility of the concomitant formation of calculus deposits and caries from in situ dentin caries model for short time periods. Six volunteers wore palatal removal appliances with four polished dentin specimens protected from intra-oral mechanical forces for up to 14 days. Each volunteer applied a 50% sucrose solution (four times a day) on the specimens and performed a daily mouthwash with 0.05% NaF. Samples were removed after 2, 5, 9 and 14 days in situ. Demineralization was analyzed by stereomicroscopy and SEM (secondary electrons and backscattered electrons modes) and calculus was analyzed by energy dispersive spectroscopy and fluorescence spectroscopy. Seventeen samples, at least one sample from each volunteer, presented dental calculus on both carious and non-carious ones, detected in all time intervals. Ca/P ratios of dental calculus ranged from 1.1 to 1.7. Some large calculus deposits on carious surfaces were confirmed by fluorescence. In conclusion, concomitant caries and calculus formation can be found in dentin caries formed in situ. This has important repercussions on the study of surface phenomena on the interface between hard dental tissues and dental plaque.
authors.affiliation: Departmento de Morfologia, Centro de Ciências da Saúde, Universidade Federal da Paraíba, Cidade Universitária, 58051-900, João Pessoa, Paraíba, Brazil.
authors.forename: Frederico B
authors.initials: FB
authors.lastname: de Sousa
authors.affiliation: Departmento de Morfologia, Centro de Ciências da Saúde, Universidade Federal da Paraíba, Cidade Universitária, 58051-900, João Pessoa, Paraíba, Brazil.
authors.forename: Pablo J
authors.initials: PJ
authors.lastname: Mangueira
authors.affiliation: Faculdade de Odontologia, Universidade do Vale do Itajaí, Itajaí, 88302-202, Santa Catarina, Brazil.
authors.forename: David R
authors.initials: DR
authors.lastname: Tames
authors.affiliation: Departamento de Física, Centro de Ciências Exatas e da Natureza, Universidade Federal de Pernambuco, Cidade Universitária, 50670-901, Recife, Pernambuco, Brazil.
authors.forename: Sandra S
authors.initials: SS
authors.lastname: Vianna
authors.affiliation: Laboratório de Imunopatologia Keizo-Asami (LIKA), Centro de Ciências Biológicas, Cidade Universitária, 50670-901, Recife, Pernambuco, Brazil.
authors.forename: Neriede S
authors.initials: NS
authors.lastname: Santos-Magalhaes