title: Prevalence, distribution, and viral burden of all 15 high-risk human papillomavirus types in adenosquamous carcinoma of the uterine cervix: a multiplex real-time polymerase chain reaction-based study.
abstract: Human papillomavirus (HPV) 16 and 18 are the types most commonly found in cervical adenosquamous carcinoma. Multiple HPV types have been found in cervical adenocarcinoma but not in the adenosquamous variant. Type-specific detection of high-risk (HR) HPV allows the detection of co-infection by multiple HPV types and assessment of viral load per cell. Our aim was to identify and quantify all HR HPV types in cervical adenosquamous carcinoma and to correlate viral loads with prognosis-related histologic features. All 15 HR HPV types were tested for by multiplex real-time polymerase chain reaction, and standard curves were created for each type. Viral loads were determined retrospectively. Prognosis-related histologic features were correlated with specific HPV types and the viral loads. A total of 80% of the tumors examined expressed HPV. Types 16/18 were detected in 86% of these cases, whereas the remaining 14% of the positive cases were infected by other types. A single type of virus was detected in 67% of cases, 2 in 29%, and 3 in 4%. Poor prognostic features were seen in 84.6% of the tumors infected with HPV 16, 46% of those infected with HPV 18, and 100% of those infected with other types. As expected, HPV 16, HPV 18, or both were the most frequent viral types; HPV 73 was the next most frequent type. Multiple HPV types were detected in 33% of the tumors. Non-HPV 16/18 cases had low viral loads, but all of these had poor prognosis-related histologic features. Two of the three recurrent cases had multiple viral types.
authors.affiliation: Department of Pathology, Women & Infants Hospital, The Warren Alpert Medical School of Brown University, Providence, RI 02905. Electronic address: mquddus@wihri.org.
authors.forename: M Ruhul
authors.initials: MR
authors.lastname: Quddus
authors.affiliation: Physicians Reference Laboratory, LLC, Overland Park, KS, 66210.
authors.forename: Pradip
authors.initials: P
authors.lastname: Manna
authors.affiliation: Department of Pathology, Women & Infants Hospital, The Warren Alpert Medical School of Brown University, Providence, RI 02905.
authors.forename: C James
authors.initials: CJ
authors.lastname: Sung
authors.affiliation: Physicians Reference Laboratory, LLC, Overland Park, KS, 66210.
authors.forename: Spencer
authors.initials: S
authors.lastname: Kerley
authors.affiliation: Department of Pathology, Women & Infants Hospital, The Warren Alpert Medical School of Brown University, Providence, RI 02905.
authors.forename: Margaret M
authors.initials: MM
authors.lastname: Steinhoff
authors.affiliation: Department of Pathology, Women & Infants Hospital, The Warren Alpert Medical School of Brown University, Providence, RI 02905.
authors.forename: W Dwayne
authors.initials: WD
authors.lastname: Lawrence
mesh_terms: D000328:Adult; D000368:Aged; D018196:Carcinoma, Adenosquamous / Q000453:epidemiology* / Q000821:virology*; D002584:Cervix Uteri / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D052162:Human papillomavirus 16; D052161:Human papillomavirus 18; D006801:Humans; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction; D027383:Papillomaviridae / Q000235:genetics; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology*; D015995:Prevalence; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology* / Q000821:virology*; D019562:Viral Load
chemical_list: D004279:DNA, Viral
keywords: Adenosquamous carcinoma; Cervical cancer; Human papillomavirus