title: Analytical determination of flavonoids aimed to analysis of natural samples and active packaging applications.
abstract: Several HPLC and UHPLC developed methods were compared to analyse the natural antioxidants catechins and quercetin used in active packaging and functional foods. Photodiode array detector coupled with a fluorescence detector and compared with LTQ-Orbitrap-MS was used. UHPLC was investigated as quick alternative without compromising the separation, analysis time shortened up to 6-fold. The feasibility of the four developed methods was compared. Linearity up to 0.9995, low detection limits (between 0.02 and 0.7 for HPLC-PDA, 2 to 7-fold lower for HPLC- LTQ-Orbitrap-MS and from 0.2 to 2mgL(-)(1) for UHPLC-PDA) and good precision parameters (RSD lower than 0.06%) were obtained. All methods were successfully applied to natural samples. LTQ-Orbitrap-MS allowed to identify other analytes of interest too. Good feasibility of the methods was also concluded from the analysis of catechin and quercetin release from new active packaging materials based on polypropylene added with catechins and green tea.
authors.affiliation: Grupo de Polímeros-Centro de Investigacións Tecnológicas (CIT), Universidade de A Coruña, Campus de Esteiro s/n, 15403 Ferrol, Spain.
authors.forename: María del Mar
authors.initials: Mdel M
authors.lastname: Castro-López
authors.affiliation: Grupo de Polímeros-Centro de Investigacións Tecnológicas (CIT), Universidade de A Coruña, Campus de Esteiro s/n, 15403 Ferrol, Spain.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: López-Vilariño
authors.affiliation: Grupo de Polímeros-Centro de Investigacións Tecnológicas (CIT), Universidade de A Coruña, Campus de Esteiro s/n, 15403 Ferrol, Spain. Electronic address: victoria.gonzalez.rodriguez@udc.es.
authors.forename: María Victoria
authors.initials: MV
authors.lastname: González-Rodríguez
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D002099:Cacao / Q000737:chemistry*; D028241:Camellia sinensis / Q000737:chemistry*; D002392:Catechin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis; D018857:Food Packaging / Q000295:instrumentation*; D007700:Kinetics; D010936:Plant Extracts / Q000032:analysis*; D010969:Plastics / Q000032:analysis*; D011794:Quercetin / Q000032:analysis*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; D010969:Plastics; D002392:Catechin; D011794:Quercetin
keywords: Active packaging; Catechins; Chromatography; Comparison; Natural antioxidants; Quercetin