title: Direct TLC/MALDI-MS coupling for modified polyamidoamine dendrimers analyses.
abstract: Polyamidoamine (PAMAM) are synthetic dendrimers which present attractive properties for the biological and biomedical fields, as they proved to be efficient drug and gene carriers. In order to increase their transfection efficiency, chemical modifications of the amino end-groups had been reported. In this work, the synthesis of the ammonia-cored G1(N) PAMAM and the consecutive chemical modification with glycine or phenylalanine amino-acids were monitored using the coupling of thin layer chromatography (TLC) with matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS). Thus, the monitoring of the PAMAM synthesis included the identification of the by-products such as defective structures of PAMAM dendrimers as well as the study of phenylalanine-grafted PAMAM oligomer distribution.
authors.affiliation: Normandie Université, COBRA, UMR6014 and FR3038, Université de Rouen, INSA de Rouen, CNRS, IRCOF, 1 rue Tesnière, 76821 Mont-Saint-Aignan Cedex, France.
authors.forename: Emma-Dune
authors.initials: ED
authors.lastname: Leriche
authors.affiliation: Normandie Université, COBRA, UMR6014 and FR3038, Université de Rouen, INSA de Rouen, CNRS, IRCOF, 1 rue Tesnière, 76821 Mont-Saint-Aignan Cedex, France. Electronic address: marie.hubert@univ-rouen.fr.
authors.forename: Marie
authors.initials: M
authors.lastname: Hubert-Roux
authors.affiliation: University of Southampton, School of chemistry, Highfield, Hants, SO17 1BJ Southampton, UK.
authors.forename: Martin C
authors.initials: MC
authors.lastname: Grossel
authors.affiliation: Normandie Université, COBRA, UMR6014 and FR3038, Université de Rouen, INSA de Rouen, CNRS, IRCOF, 1 rue Tesnière, 76821 Mont-Saint-Aignan Cedex, France.
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Lange
authors.affiliation: Normandie Université, COBRA, UMR6014 and FR3038, Université de Rouen, INSA de Rouen, CNRS, IRCOF, 1 rue Tesnière, 76821 Mont-Saint-Aignan Cedex, France.
authors.forename: Carlos
authors.initials: C
authors.lastname: Afonso
authors.affiliation: Normandie Université, COBRA, UMR6014 and FR3038, Université de Rouen, INSA de Rouen, CNRS, IRCOF, 1 rue Tesnière, 76821 Mont-Saint-Aignan Cedex, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Loutelier-Bourhis
mesh_terms: D000641:Ammonia / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002855:Chromatography, Thin Layer; D050091:Dendrimers / Q000737:chemistry*; D005998:Glycine / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000032:analysis; D010649:Phenylalanine / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D050091:Dendrimers; D004364:Pharmaceutical Preparations; C531249:Poly(amidoamine); D011073:Polyamines; D010649:Phenylalanine; D000641:Ammonia; D005998:Glycine
keywords: MALDI; Polyamidoamine dendrimers; TLC; Tandem mass spectrometry