title: Evaluation of current and new biomarkers in severe preeclampsia: a microarray approach reveals the VSIG4 gene as a potential blood biomarker.
abstract: Preeclampsia is a placental disease characterized by hypertension and proteinuria in pregnant women, and it is associated with a high maternal and neonatal morbidity. However, circulating biomarkers that are able to predict the prognosis of preeclampsia are lacking. Thirty-eight women were included in the current study. They consisted of 19 patients with preeclampsia (13 with severe preeclampsia and 6 with non-severe preeclampsia) and 19 gestational age-matched women with normal pregnancies as controls. We measured circulating factors that are associated with the coagulation pathway (including fibrinogen, fibronectin, factor VIII, antithrombin, protein S and protein C), endothelial activation (such as soluble endoglin and CD146), and the release of total and platelet-derived microparticles. These markers enabled us to discriminate the preeclampsia condition from a normal pregnancy but were not sufficient to distinguish severe from non-severe preeclampsia. We then used a microarray to study the transcriptional signature of blood samples. Preeclampsia patients exhibited a specific transcriptional program distinct from that of the control group of women. Interestingly, we also identified a severity-related transcriptional signature. Functional annotation of the upmodulated signature in severe preeclampsia highlighted two main functions related to "ribosome" and "complement". Finally, we identified 8 genes that were specifically upmodulated in severe preeclampsia compared with non-severe preeclampsia and the normotensive controls. Among these genes, we identified VSIG4 as a potential diagnostic marker of severe preeclampsia. The determination of this gene may improve the prognostic assessment of severe preeclampsia.
authors.affiliation: Aix-Marseille Université, Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, CNRS UMR 7278, INSERM U1095, Marseille, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Textoris
authors.affiliation: Aix-Marseille Université, Department of Gynecology and Obstetrics, Hôpital Nord, Marseille, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Ivorra
authors.affiliation: Aix-Marseille Université, Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, CNRS UMR 7278, INSERM U1095, Marseille, France.
authors.forename: Amira
authors.initials: A
authors.lastname: Ben Amara
authors.affiliation: Aix-Marseille Université, Laboratoire de Physiopathologie de l'Endothélium, Unité mixte de recherche, S 608 INSERM, Marseille, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Sabatier
authors.affiliation: Aix-Marseille Université, Department of Gynecology and Obstetrics, Hôpital Nord, Marseille, France.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Ménard
authors.affiliation: Aix-Marseille Université, Department of Gynecology and Obstetrics, Hôpital de la Conception, Marseille, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Heckenroth
authors.affiliation: Aix-Marseille Université, Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, CNRS UMR 7278, INSERM U1095, Marseille, France ; Aix-Marseille Université, Department of Gynecology and Obstetrics, Hôpital Nord, Marseille, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Bretelle
authors.affiliation: Aix-Marseille Université, Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, CNRS UMR 7278, INSERM U1095, Marseille, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Mege
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D006801:Humans; D058977:Molecular Sequence Annotation; D011225:Pre-Eclampsia / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D011247:Pregnancy; D011379:Prognosis; D011951:Receptors, Complement / Q000097:blood / Q000235:genetics*; D012307:Risk Factors
chemical_list: D015415:Biomarkers; D011951:Receptors, Complement; C414427:VSIG4 protein, human