title: A highly sensitive ratiometric fluorescent probe with a large emission shift for imaging endogenous cysteine in living cells.
abstract: A new design strategy for the construction of ratiometric fluorescent probe with a large emission shift was developed. Based on this strategy, a highly selective and sensitive colorimetric and ratiometic fluorescent probe for cysteine (Cys) with a 117 nm red-shifted emission was synthesized and applied to the ratiometric imaging of endogenous Cys in living cells.
authors.affiliation: School of Resources and Environment, University of Jinan, Shandong Provincial Engineering Technology Research Center for Ecological Carbon Sink and Capture Utilization, Jinan 250022, China. Electronic address: lcyzbc@163.com.
authors.forename: Baocun
authors.initials: B
authors.lastname: Zhu
authors.affiliation: School of Resources and Environment, University of Jinan, Shandong Provincial Engineering Technology Research Center for Ecological Carbon Sink and Capture Utilization, Jinan 250022, China.
authors.forename: Bingpeng
authors.initials: B
authors.lastname: Guo
authors.affiliation: School of Resources and Environment, University of Jinan, Shandong Provincial Engineering Technology Research Center for Ecological Carbon Sink and Capture Utilization, Jinan 250022, China.
authors.forename: Yunzhou
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: School of Resources and Environment, University of Jinan, Shandong Provincial Engineering Technology Research Center for Ecological Carbon Sink and Capture Utilization, Jinan 250022, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.affiliation: School of Resources and Environment, University of Jinan, Shandong Provincial Engineering Technology Research Center for Ecological Carbon Sink and Capture Utilization, Jinan 250022, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Du
mesh_terms: D000818:Animals; D002460:Cell Line; D003545:Cysteine / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D053644:Naphthalimides / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D053644:Naphthalimides; D003545:Cysteine
keywords: Bioimaging; Cysteine; Hydroxynaphthalimide; Large emission shift; Ratiometric fluorescent probe