title: Analysis of isorhamnetin-3-O-neohesperidoside in rat plasma by liquid chromatography/electrospray ionization tandem mass spectrometry and its application to pharmacokinetic studies.
abstract: OBJECTIVE
  To establish an LC-MS/MS method for determination of isorhamnetin-3-O-neohesperidoside and investigate its application on pharmacokinetic study in rats.
  
  
  METHODS
  Eight rats were given 5 mg·kg(-1) isorhamnetin-3-O-neohesperidoside after intravenous administration. A highly sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of isorhamnetin-3-O-neohesperidosidein rat plasma using rutin as internal standard. The analytes and rutin (internal standard) were extracted with methanol followed by a rapid isocratic elution with 10 mmol·L(-1) ammonium acetate buffer/methanol (20 : 80, V/V) on a C18 column (150 mm × 2.1 mm, I.D., 5 μm) and subsequent analysis by mass spectrometry in the multi-eaction-monitoring mode.
  
  
  RESULTS
  The assays were linear over the concentration range of 0.01-10 μg·mL(-1) for isorhamnetin-3-O-neohesperidosidein rat plasma. The lower limit of quantifications for isorhamnetin-3-O-neohesperidoside was 0.01 μg·mL(-1).
  
  
  CONCLUSIONS
  The validated method is successfully applied to determine the plasma concentrations of isorhamnetin-3-O-neohesperidosidein in rats.
authors.affiliation: Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Shi-Jia
authors.initials: SJ
authors.lastname: Liu
authors.affiliation: Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
authors.forename: Pei-Dong
authors.initials: PD
authors.lastname: Chen
authors.affiliation: Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Dai
authors.affiliation: Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China. Electronic address: snow8185@yahoo.com.cn.
authors.forename: Wen-Zheng
authors.initials: WZ
authors.lastname: Ju
authors.affiliation: Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Xie
authors.affiliation: Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.affiliation: Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
authors.forename: An-Wei
authors.initials: AW
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: boyangyu59@163.com.
authors.forename: Bo-Yang
authors.initials: BY
authors.lastname: Yu
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D044948:Flavonols / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics; D011058:Pollen / Q000737:chemistry; D051381:Rats; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D032401:Typhaceae / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D044948:Flavonols; C469309:isorhamnetin-3-O-neohesperidoside
keywords: Electrospray ionization; Isorhamnetin-3-O-neohesperidoside; LC-MS/MS; Pharmacokinetics; Rat plasma