title: Malignant cells derived from 3T3 fibroblast feeder layer in cell culture for nasopharyngeal carcinoma.
abstract: In this study, we discovered a subpopulation of 3T3 feeder cells were malignantly transformed by nasopharyngeal carcinoma (NPC) tumor cells during co-culture. The transformed 3T3 cells acquired an accelerated growth rate, displayed loosely attached multilayer growth in vitro and highly tumorigenic in vivo. Most strikingly, instead of forming sarcomas, they developed into carcinoma-like tumors somewhat resembling the original NPC. We further demonstrated the transformation is not a single isolated event, rather a common reproducible, cell contact dispensable phenomena among NPC tumor cells. However, NPC tumor cells alone were not sufficient to confer the transformed characteristics onto normal human cells.
authors.affiliation: Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore. Electronic address: entyf@nus.edu.sg.
authors.forename: Fenggang
authors.initials: F
authors.lastname: Yu
authors.affiliation: Cancer Science Institute of Singapore, National University of Singapore, Singapore.
authors.forename: Wen-son
authors.initials: WS
authors.lastname: Hsieh
authors.affiliation: Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Fredrik
authors.initials: F
authors.lastname: Petersson
authors.affiliation: Cancer Science Institute of Singapore, National University of Singapore, Singapore.
authors.forename: Henry
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Chunwei
authors.initials: C
authors.lastname: Li
authors.affiliation: Cancer Science Institute of Singapore, National University of Singapore, Singapore.
authors.forename: Soon Wah
authors.initials: SW
authors.lastname: Low
authors.affiliation: Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: De-Yun
authors.initials: DY
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore; Head & Neck Tumor group, National Cancer Institute of Singapore, Head & Neck Surgery, NUHS, Singapore.
authors.forename: Kwok Seng
authors.initials: KS
authors.lastname: Loh
mesh_terms: D000818:Animals; D002277:Carcinoma; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D018920:Coculture Techniques; D061252:Feeder Cells / Q000473:pathology*; D005260:Female; D005347:Fibroblasts / Q000473:pathology / Q000637:transplantation; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D041681:NIH 3T3 Cells; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D061251:Primary Cell Culture / Q000379:methods*; D014407:Tumor Cells, Cultured
keywords: 3T3 cells; Cell contact; Feeder layer; Nasopharyngeal carcinoma; Transform