title: An amperometric β-glucan biosensor based on the immobilization of bi-enzyme on Prussian blue-chitosan and gold nanoparticles-chitosan nanocomposite films.
abstract: A novel β-glucan biosensor was fabricated by immobilizing β-glucanase (β-G) with glucose oxidase (GOD) on nano-Prussian blue-chitosan (PB-CS) and gold nanoparticles-chitosan (AuNPs-CS) composites. Both the PB-CS and AuNPs_CS film were directly electrodeposited on the surface of gold electrode. The morphology of the AuNPs-CS/PB-CS nanocomposites was characterized by scanning electron microscope (SEM). The electrochemical behavior of the resulting sensor was investigated using cyclic voltammetry (CV) and amperometry. It was found that PB-CS nanocomposite exhibited an excellent electrocatalytic reduction towards hydrogen peroxide at a low applied potential window. The synergistic effect of AuNPs-CS/PB-CS nanocomposites could remarkably improve the performances of the biosensor. Under optimal conditions, the biosensor showed a wide linear range of 6.25-93.75 μM, with a correlation coefficient of 0.9991. The sensitivity at an applied potential of 0.0 V was 100 nA μM(-1) cm(-2), with a detection limit of 1.56 μM. The apparent Michaelis-Menten constant (Km) was found to be 1.0mM, showed a high affinity of the immobilizing β-G for β-glucan. The biosensor displayed a rapid response (within 10s) toward β-glucan, with a good selectivity and stability.
authors.affiliation: Department of Medical Chemistry, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Medical Chemistry, Hebei Medical University, Shijiazhuang 050017, China. Electronic address: xuepingji@126.com.
authors.forename: Xueping
authors.initials: X
authors.lastname: Ji
authors.affiliation: Department of Medical Chemistry, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Medical Chemistry, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Medical Chemistry, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Xianrui
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Medical Chemistry, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Ruixing
authors.initials: R
authors.lastname: Ni
authors.affiliation: Department of Medical Chemistry, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Yuheng
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation*; D048271:Chitosan / Q000737:chemistry; D003217:Conductometry / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005295:Ferrocyanides / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D047071:beta-Glucans / Q000032:analysis* / Q000737:chemistry; D001617:beta-Glucosidase / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D005295:Ferrocyanides; D047071:beta-Glucans; D006046:Gold; D048271:Chitosan; D005949:Glucose Oxidase; D001617:beta-Glucosidase; C000170:ferric ferrocyanide
keywords: Biosensor; Chitosan; Gold nanoparticles; Prussian blue; β-glucan; β-glucanase