title: Evaluation of the activity of thermostable DNA polymerases in the presence of heme, as a key inhibitor in the real time PCR method in diagnostics of sepsis.
abstract: The study aim was evaluation of the usefulness of several thermostable DNA polymerases in real time PCR conducted in the presence of the heme. Our study had the advantage of testing several different polymerases, one of which proved to be the least sensitive to heme activity. We also found that there is no need of supplementing the reaction mixture with protective substances like BSA. Selection of the appropriate polymerase can increase the efficiency of the PCR reaction which is very important for diagnosis of sepsis and for other analyses performed on DNA template isolated from the blood.
authors.affiliation: Chair of Microbiology, Jagiellonian University Medical College, Kraków, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Gosiewski
authors.affiliation: Chair of Microbiology, Jagiellonian University Medical College, Kraków, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Brzychczy-Włoch
authors.affiliation: Chair of Microbiology, Jagiellonian University Medical College, Kraków, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Pietrzyk
authors.affiliation: Chair of Microbiology, Jagiellonian University Medical College, Kraków, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Sroka
authors.affiliation: Chair of Microbiology, Jagiellonian University Medical College, Kraków, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Bulanda
mesh_terms: D004259:DNA-Directed DNA Polymerase / Q000097:blood*; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D006418:Heme / Q000378:metabolism*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D018805:Sepsis / Q000097:blood / Q000175:diagnosis*
chemical_list: D006418:Heme; D004259:DNA-Directed DNA Polymerase