title: Genome-wide analyses of radioresistance-associated miRNA expression profile in nasopharyngeal carcinoma using next generation deep sequencing.
abstract: BACKGROUND
  Rapidly growing evidence suggests that microRNAs (miRNAs) are involved in a wide range of cancer malignant behaviours including radioresistance. Therefore, the present study was designed to investigate miRNA expression patterns associated with radioresistance in NPC.
  
  
  METHODS
  The differential expression profiles of miRNAs and mRNAs associated with NPC radioresistance were constructed. The predicted target mRNAs of miRNAs and their enriched signaling pathways were analyzed via biological informatical algorithms. Finally, partial miRNAs and pathways-correlated target mRNAs were validated in two NPC radioreisitant cell models.
  
  
  RESULTS
  50 known and 9 novel miRNAs with significant difference were identified, and their target mRNAs were narrowed down to 53 nasopharyngeal-/NPC-specific mRNAs. Subsequent KEGG analyses demonstrated that the 53 mRNAs were enriched in 37 signaling pathways. Further qRT-PCR assays confirmed 3 down-regulated miRNAs (miR-324-3p, miR-93-3p and miR-4501), 3 up-regulated miRNAs (miR-371a-5p, miR-34c-5p and miR-1323) and 2 novel miRNAs. Additionally, corresponding alterations of pathways-correlated target mRNAs were observed including 5 up-regulated mRNAs (ICAM1, WNT2B, MYC, HLA-F and TGF-β1) and 3 down-regulated mRNAs (CDH1, PTENP1 and HSP90AA1).
  
  
  CONCLUSIONS
  Our study provides an overview of miRNA expression profile and the interactions between miRNA and their target mRNAs, which will deepen our understanding of the important roles of miRNAs in NPC radioresistance.
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Hunan, China ; Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, Hunan, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Hunan, China ; Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, Hunan, China.
authors.forename: Yuanzheng
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Hunan, China ; Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, Hunan, China.
authors.forename: Zhongwu
authors.initials: Z
authors.lastname: Su
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Hunan, China ; Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, Hunan, China.
authors.forename: Shuling
authors.initials: S
authors.lastname: Ren
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Hunan, China ; Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, Hunan, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Hunan, China ; Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, Hunan, China.
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Hunan, China ; Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, Hunan, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
mesh_terms: D001483:Base Sequence; D002277:Carcinoma; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology*; D011836:Radiation Tolerance / Q000235:genetics*; D017423:Sequence Analysis, RNA
chemical_list: D035683:MicroRNAs