title: [Effect of propranolol on dialysate myoglobin levels evoked by ischemia/reperfusion].
abstract: BACKGROUND
  Although propranolol is well known to induce the preventive effect against ischemic disease, its effect on ischemia/reperfusion injuries remains unknown. We examined whether propranolol provides cardioprotection against ischemia/reperfusion injuries.
  
  
  METHODS
  Microdialysis and 30 min of coronary artery occlusion followed by 60 min of reperfusion were applied in anesthetized rats. The dialysate myoglobin levels served as indices of myocardial injury, and were measured in the following groups: without propranolol administration (vehicle); with propranolol (2 mg x kg(-1), pro(2)-pre), (5 mg x kg(-1), pro (5) -pre) administration; zatebradine (0.5 mg x kg(-1), zat-pre) administration. Drugs were administered before coronary occlusion.
  
  
  RESULTS
  In the pro(2)-pre, pro(5)-pre and zat-pre groups the heart rate was suppressed during the entire period. Ischemia-induced increase in dialysate myoglobin level was suppressed in the pro(2)-pre, pro(5)-pre and zat-pre groups in comparison with vehicle group. Reperfusion-induced increase in dialysate myoglobin level was suppressed in the pro(2)-pre, pro(5)-pre and zat-pre groups in comparison with vehicle group. The ratios of reperfusion/ischemia myoglobin level in pro (2)-pre and pro(5)-pre were lower than those in vehicle and zat-pre. Reperfusion-induced increase in myoglobin level was suppressed by administration of propranolol immediately before reperfusion.
  
  
  CONCLUSIONS
  The results suggest that propranolol provides myocardial protection against ischemia/reperfusion injury. The protective effect of propranolol on reperfusion might be independent of its effect on ischemia.
authors.affiliation: Department of Anesthesiology, Shiga University of Medical Science, Otsu 520-2192.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Sawada
authors.affiliation: Department of Anesthesiology, Shiga University of Medical Science, Otsu 520-2192.
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Kitagawa
authors.affiliation: Department of Anesthesiology, Shiga University of Medical Science, Otsu 520-2192.
authors.forename: Shuich
authors.initials: S
authors.lastname: Nosaka
mesh_terms: D000208:Acute Disease; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000818:Animals; D002316:Cardiotonic Agents; D015314:Dialysis Solutions / Q000737:chemistry*; D006339:Heart Rate / Q000187:drug effects; D008297:Male; D017551:Microdialysis; D017202:Myocardial Ischemia / Q000378:metabolism* / Q000517:prevention & control; D015428:Myocardial Reperfusion Injury / Q000378:metabolism* / Q000517:prevention & control; D009211:Myoglobin / Q000378:metabolism*; D011433:Propranolol / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000319:Adrenergic beta-Antagonists; D002316:Cardiotonic Agents; D015314:Dialysis Solutions; D009211:Myoglobin; D011433:Propranolol