title: [Engineering Saccharomyces cerevisiae for sclareol production].
abstract: Sclareol is a member of labdane type diterpenes mostly used as fragrance ingredient. To enable microbial production of sclareol, synthetic pathways were constructed by incorporating labdenediol diphosphate synthase (LPPS) and terpene synthase (TPS) of the plant Salvia sclarea into Saccharomyces cerevisiae. It was found that sclareol production could be benefited by overexpression of key enzyme for precursor biosynthesis, construction of fusion protein for substrate channeling, and removal of signal peptides from LPPS and TPS. Under optimal shake flask culture conditions, strain S6 produced 8.96 mg/L sclareol. These results provided useful information for development of heterologous hosts for production of terpenoids.
authors.affiliation: Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.affiliation: Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China.
authors.forename: Yongjin
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China.
authors.forename: Wujun
authors.initials: W
authors.lastname: Liu
authors.affiliation: Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Shen
authors.affiliation: Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China.
authors.forename: Zongbao K
authors.initials: ZK
authors.lastname: Zhao
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000096:biosynthesis* / Q000235:genetics; D004224:Diterpenes / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D027544:Salvia / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics
chemical_list: D004224:Diterpenes; D011993:Recombinant Fusion Proteins; C070760:sclareol; D019883:Alkyl and Aryl Transferases; C108877:terpene synthase