title: [Effect of IL-18BP on Fractalkine chemokine expression in the kidney tissue of rats with renal fibrosis].
abstract: OBJECTIVE
  To study the expression of Fractalkine (FKN) in the kidney tissue of rats with renal fibrosis and the effect of IL-18BP on FKN.
  
  
  METHODS
  Male Wister rats were randomly assigned to sham-operation (n=24), unilatral ureteral obstruction (UUO, n=22), and IL-18 binding protein (IL-18BP) treatment groups (n=23). The UUO model was prepared by unilateral ureteral ligation in the later two groups. The IL-18BP treatment group received an intraperitoneal injection of IL-18BP (0.1 mg/kg) every other day after UUO inducement, for 7 times, while normal saline was administered in the other two groups. Seven or eight rats of every group were sacrificed at 3, 7 or 14 days after IL-18BP or normal saline injections. FKN levels at various times were detected by immunohistochemistry and Western blot.
  
  
  RESULTS
  Compared with the sham-operation group, FKN levels in the kidney tissue of the untreated UUO group increased significantly at all time points (P<0.01). IL-18BP treatment decreased significantly FKN levels in the kidney tissue at all time points compared with the untreated UUO group (P<0.01).
  
  
  CONCLUSIONS
  IL-18BP treatment may down-regulate the increased FKN levels of the rat kidney tissue caused by UUO, possibly thus delays the occurrence and development of renal fibrosis.
authors.affiliation: Department of Pediatrics, First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang 154003, China. wanglimin6518@163.com.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Wang
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Li
authors.forename: Jia-Bin
authors.initials: JB
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Ying-Jiao
authors.initials: YJ
authors.lastname: Chi
authors.forename: Jing-Wei
authors.initials: JW
authors.lastname: Yuan
authors.forename: Ying-Jie
authors.initials: YJ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D054428:Chemokine CX3CL1 / Q000235:genetics*; D005355:Fibrosis; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D007668:Kidney / Q000276:immunology / Q000473:pathology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014517:Ureteral Obstruction / Q000276:immunology
chemical_list: D054428:Chemokine CX3CL1; C515540:Cx3cl1 protein, rat; D036341:Intercellular Signaling Peptides and Proteins; C118370:interleukin-18 binding protein