title: [Comparison of ELISA and pharmacia UNICAP detection reagent for house dust mite specific IgG4].
abstract: OBJECTIVE
  To compare the ELISA and Pharmica unicap reagents of Dermatophagoides pteronyssinus specific IgG4 in clinic.
  
  
  METHODS
  The stability of ELISA reagent was studied first with parameters of intra and inter assay variations (%CV). Calibration curve of the two reagents was compared. Sixty-five serum samples before or after standardized specific immunotherapy were examined with the two methods, correlation coefficient was calculated.
  
  
  RESULTS
  The stability of ELISA reagent was good with low intra and inter assay variations (3.48% and 5.78%). Calibration curve of the two reagents was similar. The correlation coefficient was 0.93 between the two test methods.
  
  
  CONCLUSIONS
  The results of ELISA and Pharmica unicap reagents of Dermatophagoides pteronyssinus specific IgG4 were highly consistent. The ELISA reagents could be applied in clinic.
authors.affiliation: Jiangsu Huaguan Biotechnology Joint Stock Company Ltd, Taizhou, 225300, China.
authors.forename: Genyu
authors.initials: G
authors.lastname: Luo
authors.affiliation: Hubei Extny Exit Inspection and Quarantine Bureau.
authors.forename: Rui
authors.initials: R
authors.lastname: Gong
authors.affiliation: Jiangsu Huaguan Biotechnology Joint Stock Company Ltd, Taizhou, 225300, China.
authors.forename: Zhenkui
authors.initials: Z
authors.lastname: Gong
authors.affiliation: Jiangsu Huaguan Biotechnology Joint Stock Company Ltd, Taizhou, 225300, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.affiliation: Jiangsu Huaguan Biotechnology Joint Stock Company Ltd, Taizhou, 225300, China.
authors.forename: Changyong
authors.initials: C
authors.lastname: Zou
authors.affiliation: Department of Allergy, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology.
authors.forename: Rongfei
authors.initials: R
authors.lastname: Zhu
authors.affiliation: Department of Otorhinolaryngology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D002138:Calibration; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007202:Indicators and Reagents; D039981:Pyroglyphidae / Q000276:immunology
chemical_list: D007074:Immunoglobulin G; D007202:Indicators and Reagents