title: Expression and accumulation of the two-domain odorant-binding protein AaegOBP45 in the ovaries of blood-fed Aedes aegypti.
abstract: BACKGROUND
  Aedes aegypti mosquitoes are the main vectors of dengue viruses. Despite global efforts to reduce the prevalence of dengue using integrated vector management strategies, innovative alternatives are necessary to help prevent virus transmission. Detailed characterizations of Ae. aegypti genes and their products provide information about the biology of mosquitoes and may serve as foundations for the design of new vector control methods.
  
  
  RESULTS
  We studied the Ae. aegypti gene, AAEL010714, that encodes a two-domain odorant-binding protein, AaegOBP45. The predicted gene structure and sequence were validated, although single nucleotide polymorphisms were observed. Transcriptional and translational products accumulate in the ovaries of blood fed females and are not detected or are at low abundance in other tissues.
  
  
  CONCLUSIONS
  We validated the Ae. aegypti AAEL010714 gene sequence and characterized the expression profile of a two-domain OBP expressed in ovaries. We propose that AaegOBP45 function as a component of the mosquito eggshell.
authors.affiliation: Departamento de Parasitologia, Laboratório de Mosquitos Geneticamente Modificados, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP 05508-000, Brazil. alcosta@icb.usp.br.
authors.forename: André Luis
authors.initials: AL
authors.lastname: Costa-da-Silva
authors.forename: Bianca B
authors.initials: BB
authors.lastname: Kojin
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Marinotti
authors.forename: Anthony A
authors.initials: AA
authors.lastname: James
authors.forename: Margareth Lara
authors.initials: ML
authors.lastname: Capurro
mesh_terms: D000330:Aedes / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010053:Ovary / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013997:Time Factors
chemical_list: D019476:Insect Proteins