title: Identification of 4″-isovaleryl-spiramycin III produced by Bacillus sp. fmbJ.
abstract: The production of secondary metabolites with antibiotic properties is a common characteristic to Bacillus spp. These metabolites not only have diverse chemical structures but also have a wide range of bioactivities with medicinal and agricultural interests such as antibiotic. Bacillus sp. fmbJ has been found to produce lipopeptides fengycin and surfactin in accordance with our previous report. In this study, another antimicrobial substance was separated and purified from the culture supernatant of strain fmbJ using the silica gel column chromatography and preparative reversed-phase high-performance liquid chromatography. By means of electrospray ionization mass spectroscopy, infrared spectroscopy, and nuclear magnetic resonance, the antagonistic compound was determined to be 4″-isovaleryl-spiramycin III with the molecular weight of 982 Da. This report is the first to introduce the finding of spiramycin produced from Bacillus sp. The study provides a novel source for the production of spiramycin in pharmaceutical industries.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Key Laboratory of Food Processing and Quality Control, Ministry of Agriculture of China, 1 Weigang, Nanjing, 210095, China, dengyang719@hotmail.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Deng
authors.forename: Yanjuan
authors.initials: Y
authors.lastname: Ju
authors.forename: Zhaoxin
authors.initials: Z
authors.lastname: Lu
authors.forename: Fengxia
authors.initials: F
authors.lastname: Lu
authors.forename: Dong
authors.initials: D
authors.lastname: Yan
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Bie
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D001407:Bacillus / Q000378:metabolism*; D015394:Molecular Structure; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular; D021241:Spectrometry, Mass, Electrospray Ionization; D017550:Spectroscopy, Fourier Transform Infrared; D015572:Spiramycin / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: C586971:4''-isovaleryl-spiramycin III; D000900:Anti-Bacterial Agents; C087816:spiramycin III; D015572:Spiramycin