title: Circulating C19MC microRNAs in preeclampsia, gestational hypertension, and fetal growth restriction.
abstract: The objective of the study was to identify the profile of circulating C19MC microRNAs (miR-516-5p, miR-517*, miR-518b, miR-520a*, miR-520h, miR-525, and miR-526a) in patients with established preeclampsia (n = 63), fetal growth restriction (n = 27), and gestational hypertension (n = 23). We examined the correlation between plasmatic concentrations and expression levels of microRNAs and the severity of the disease with respect to clinical signs, requirements for the delivery, and Doppler ultrasound parameters. Using absolute and relative quantification approaches, increased extracellular C19MC microRNA levels (miR-516-5p, P = 0.037, P = 0.009; miR-517*, P = 0.033, P = 0.043; miR-520a*, P = 0.001, P = 0.009; miR-525, P = 0.026, P = 0.01; miR-526a, P = 0.03, P = 0.035) were detected in patients with preeclampsia. The association analysis pointed to no relationship between C19MC microRNA plasmatic concentrations and expression profile and identified risk factors for a poorer perinatal outcome. However, the dependence between the levels of plasmatic C19MC microRNAs and the pulsatility index in the middle cerebral artery and the values of cerebroplacental ratio was demonstrated. The study brought the interesting finding that the upregulation of miR-516-5p, miR-517*, miR-520a*, miR-525, and miR-526a is a characteristic phenomenon of established preeclampsia.
authors.affiliation: Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Ruska 87, 100 00 Prague, Czech Republic.
authors.forename: Ilona
authors.initials: I
authors.lastname: Hromadnikova
authors.affiliation: Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Ruska 87, 100 00 Prague, Czech Republic.
authors.forename: Katerina
authors.initials: K
authors.lastname: Kotlabova
authors.affiliation: Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Ruska 87, 100 00 Prague, Czech Republic.
authors.forename: Marketa
authors.initials: M
authors.lastname: Ondrackova
authors.affiliation: Institute for the Care of the Mother and Child, Third Faculty of Medicine, Charles University, Podolske Nabrezi 157/36, 147 00 Prague, Czech Republic.
authors.forename: Andrea
authors.initials: A
authors.lastname: Kestlerova
authors.affiliation: Institute for the Care of the Mother and Child, Third Faculty of Medicine, Charles University, Podolske Nabrezi 157/36, 147 00 Prague, Czech Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Novotna
authors.affiliation: Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Ruska 87, 100 00 Prague, Czech Republic ; Institute for the Care of the Mother and Child, Third Faculty of Medicine, Charles University, Podolske Nabrezi 157/36, 147 00 Prague, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Hympanova
authors.affiliation: Clinic of Obstetrics and Gynecology, Second Faculty of Medicine, Charles University, V Uvalu 84, 150 06 Prague, Czech Republic.
authors.forename: Jindrich
authors.initials: J
authors.lastname: Doucha
authors.affiliation: Institute for the Care of the Mother and Child, Third Faculty of Medicine, Charles University, Podolske Nabrezi 157/36, 147 00 Prague, Czech Republic.
authors.forename: Ladislav
authors.initials: L
authors.lastname: Krofta
mesh_terms: D005260:Female; D005317:Fetal Growth Retardation / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D046110:Hypertension, Pregnancy-Induced / Q000235:genetics*; D035683:MicroRNAs / Q000097:blood*; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy / Q000097:blood; D012720:Severity of Illness Index; D018608:Ultrasonography, Doppler
chemical_list: D035683:MicroRNAs