title: Highly sensitive electrochemiluminescence determination of etamsylate using a low-cost electrochemical flow-through cell based on a tris(2, 2'-bipyridyl)ruthenium(II)-Nafion-modified carbon paste electrode.
abstract: A simple and sensitive electrochemiluminescence (ECL) method for the determination of etamsylate has been developed by coupling an electrochemical flow-through cell with a tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy)3(2+))-Nafion-modified carbon electrode. It is based on the oxidized Ru(bpy)3(2+) on the electrode surface reacting with etamsylate and producing an excellent ECL signal. Under optimized experimental conditions, the proposed method allows the measurement of etamsylate over the range of 8-1000 ng/mL with a correlation coefficient of r = 0.9997 (n = 7) and a limit of detection of 1.57 ng/mL (3σ), the relative standard deviation (RSD) for 1000 ng/mL etamsylate (n = 7) is 0.96%. The immobilized Ru(bpy)3(2+) carbon paste electrode shows good electrochemical and photochemical stability. This method is rapid, simple, sensitive and has good reproducibility. It has been successfully applied to the determination of the studied etamsylate in pharmaceutical preparations with satisfactory results. The possible ECL reaction mechanism has also been discussed.
authors.affiliation: College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Suzhou, 215123, China; The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, Suzhou, 215123, China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Rao
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Jianguo
authors.initials: J
authors.lastname: Li
mesh_terms: D002244:Carbon / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000191:economics; D004566:Electrodes; D004979:Ethamsylate / Q000032:analysis*; D005465:Fluorocarbon Polymers / Q000737:chemistry*; D049449:Luminescence; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D005465:Fluorocarbon Polymers; D009942:Organometallic Compounds; C415768:tris(2,2'-bipyridyl)ruthenium(II); D004979:Ethamsylate; C040402:perfluorosulfonic acid; D002244:Carbon
keywords: Ru(bpy)32+; electrochemiluminescence; etamsylate; flow-injection analysis; nafion-modified carbon paste electrode