title: Purification of a thermostable chitinase from Bacillus cereus by chitin affinity and its application in microbial community changes in soil.
abstract: A thermostable chitinase was purified by chitin affinity from the culture supernatant of Bacillus cereus TKU028 with shrimp head powder (SHP) as the sole carbon/nitrogen source. TKU028 chitinase was purified using a one-step affinity adsorbent system, and the molecular mass of TKU028 chitinase (approximately 40 kDa) was then determined using SDS-PAGE. The enzyme was stable for 60 min at temperatures below 60 °C and stable over a broad pH range of 4-9 for 60 min. In addition, the temporal changes of a bacterial community in mangrove river sediment of the Tamsui River with added SHP were also analysed by PCR-denaturing gradient gel electrophoresis to investigate the effects of B. cereus TKU028 on the degradation of SHP. The 6-week incubation sample of SHP and B. cereus TKU028-amended mangrove river sediment displayed the highest amount of biomass, reducing sugar and total sugar, and some variance of bacterial community composition existed in the soils.
authors.affiliation: Life Science Development Center, Tamkang University, New Taipei City, 25137, Taiwan, ltw27@ms55.hinet.net.
authors.forename: Tzu-Wen
authors.initials: TW
authors.lastname: Liang
authors.forename: Tung-Yen
authors.initials: TY
authors.lastname: Hsieh
authors.forename: San-Lang
authors.initials: SL
authors.lastname: Wang
mesh_terms: D001409:Bacillus cereus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D002688:Chitinases / Q000737:chemistry* / Q000302:isolation & purification*; D004795:Enzyme Stability; D006358:Hot Temperature
chemical_list: D001426:Bacterial Proteins; D002688:Chitinases