title: Effect of scan quality on diagnostic accuracy of spectral-domain optical coherence tomography in glaucoma.
abstract: OBJECTIVE
  To evaluate the effect of scan quality on the diagnostic accuracies of optic nerve head (ONH), retinal nerve fiber layer (RNFL), and ganglion cell complex (GCC) parameters of spectral-domain optical coherence tomography (SD OCT) in glaucoma.
  
  
  METHODS
  Cross-sectional study.
  
  
  METHODS
  Two hundred fifty-two eyes of 183 control subjects (mean deviation [MD]: -1.84 dB) and 207 eyes of 159 glaucoma patients (MD: -7.31 dB) underwent ONH, RNFL, and GCC scanning with SD OCT. Scan quality of SD OCT images was based on signal strength index (SSI) values. Influence of SSI on diagnostic accuracy of SD OCT was evaluated by receiver operating characteristic (ROC) regression.
  
  
  RESULTS
  Diagnostic accuracies of all SD OCT parameters were better when the SSI values were higher. This effect was statistically significant (P < .05) for ONH and RNFL but not for GCC parameters. In mild glaucoma (MD of -5 dB), area under ROC curve (AUC) for rim area, average RNFL thickness, and average GCC thickness parameters improved from 0.651, 0.678, and 0.726, respectively, at an SSI value of 30 to 0.873, 0.962, and 0.886, respectively, at an SSI of 70. AUCs of the same parameters in advanced glaucoma (MD of -15 dB) improved from 0.747, 0.890, and 0.873, respectively, at an SSI value of 30 to 0.922, 0.994, and 0.959, respectively, at an SSI of 70.
  
  
  CONCLUSIONS
  Diagnostic accuracies of SD OCT parameters in glaucoma were significantly influenced by the scan quality even when the SSI values were within the manufacturer-recommended limits. These results should be considered while interpreting the SD OCT scans for glaucoma.
authors.affiliation: Vazir Sultan Tobacco (VST) Glaucoma Center, L. V. Prasad Eye Institute, Banjara Hills, Hyderabad, India; Center for Clinical Epidemiology and Biostatistics, L. V. Prasad Eye Institute, Banjara Hills, Hyderabad, India. Electronic address: harshalaxmanarao@gmail.com.
authors.forename: Harsha L
authors.initials: HL
authors.lastname: Rao
authors.affiliation: Vazir Sultan Tobacco (VST) Glaucoma Center, L. V. Prasad Eye Institute, Banjara Hills, Hyderabad, India.
authors.forename: Uday K
authors.initials: UK
authors.lastname: Addepalli
authors.affiliation: Vazir Sultan Tobacco (VST) Glaucoma Center, L. V. Prasad Eye Institute, Banjara Hills, Hyderabad, India.
authors.forename: Ravi K
authors.initials: RK
authors.lastname: Yadav
authors.affiliation: Vazir Sultan Tobacco (VST) Glaucoma Center, L. V. Prasad Eye Institute, Banjara Hills, Hyderabad, India.
authors.forename: Sirisha
authors.initials: S
authors.lastname: Senthil
authors.affiliation: Vazir Sultan Tobacco (VST) Glaucoma Center, L. V. Prasad Eye Institute, Banjara Hills, Hyderabad, India.
authors.forename: Nikhil S
authors.initials: NS
authors.lastname: Choudhari
authors.affiliation: Vazir Sultan Tobacco (VST) Glaucoma Center, L. V. Prasad Eye Institute, Banjara Hills, Hyderabad, India.
authors.forename: Chandra S
authors.initials: CS
authors.lastname: Garudadri
mesh_terms: D003430:Cross-Sectional Studies; D003941:Diagnostic Techniques, Ophthalmological / Q000592:standards*; D005901:Glaucoma / Q000175:diagnosis*; D006801:Humans; D007429:Intraocular Pressure; D008875:Middle Aged; D009412:Nerve Fibers / Q000473:pathology*; D009898:Optic Disk / Q000473:pathology*; D009901:Optic Nerve Diseases / Q000175:diagnosis*; D012372:ROC Curve; D015203:Reproducibility of Results; D012165:Retinal Ganglion Cells / Q000473:pathology*; D041623:Tomography, Optical Coherence / Q000379:methods / Q000592:standards*; D014065:Tonometry, Ocular