title: Cytotoxic and apoptogenic effect of Swietenia mahagoni (L.) Jacq. leaf extract in human leukemic cell lines U937, K562 and HL-60.
abstract: The apoptogenic activity of Swietenia mahagoni leaf extract (SMLE) was investigated against three human leukemic cell lines - U937, K562 and HL-60. SMLE inhibited cell growth and metabolic activity of the leukemic cells and showed characteristic features of apoptosis. Flow-cytometric analysis showed that SMLE arrested U937 and K562 cell populations in the G2-M phase and the HL-60 cell population in the G1 phase of cell cycle. SMLE induced apoptosis was found to be mediated through mitochondrial intrinsic pathway involving the release of cytochrome c into the cytosol and activation of caspase-9 and caspase-3. Two flavonoids, catechin and quercetin-3-O-glucoside, isolated from SMLE, were found to inhibit the growth and metabolic activity of U937, K562 and HL-60 cells at much lower concentrations thus indicating that these two flavonoids might be the active ingredients responsible for the anti-leukemic activity of SMLE.
authors.affiliation: Drug Development Department, Indian Institute of Chemical Biology (A Unit of Council of Scientific & Industrial Research, India), 4 Raja S.C. Mullick Road, Kolkata 700032, India.
authors.forename: S
authors.initials: S
authors.lastname: Roy
authors.affiliation: Department of Chemistry, K.N. College, Berhampur 742101, India.
authors.forename: B
authors.initials: B
authors.lastname: Banerjee
authors.affiliation: Drug Development Department, Indian Institute of Chemical Biology (A Unit of Council of Scientific & Industrial Research, India), 4 Raja S.C. Mullick Road, Kolkata 700032, India. Electronic address: vedasiromoni@gmail.com.
authors.forename: J R
authors.initials: JR
authors.lastname: Vedasiromoni
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002392:Catechin / Q000302:isolation & purification / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation; D003576:Electron Transport Complex IV / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D029582:Meliaceae; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D011794:Quercetin / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology; D020298:U937 Cells
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; C016527:isoquercitrin; D002392:Catechin; D011794:Quercetin; D003576:Electron Transport Complex IV; D053148:Caspase 3; D053453:Caspase 9
keywords: Apoptogenic activity; Catechin; In vitro anti-leukemic activity; Mitochondria mediated intrinsic pathway; Quercetin-3-O-glucoside; Swietenia mahagoni leaf extract