title: [Research on soil bacteria under the impact of sealed CO2 leakage by high-throughput sequencing technology].
abstract: Carbon dioxide Capture and Storage has provided a new option for mitigating global anthropogenic CO2 emission with its unique advantages. However, there is a risk of the sealed CO2 leakage, bringing a serious threat to the ecology system. It is widely known that soil microorganisms are closely related to soil health, while the study on the impact of sequestered CO2 leakage on soil microorganisms is quite deficient. In this study, the leakage scenarios of sealed CO2 were constructed and the 16S rRNA genes of soil bacteria were sequenced by Illumina high-throughput sequencing technology on Miseq platform, and related biological analysis was conducted to explore the changes of soil bacterial abundance, diversity and structure. There were 486,645 reads for 43,017 OTUs of 15 soil samples and the results of biological analysis showed that there were differences in the abundance, diversity and community structure of soil bacterial community under different CO, leakage scenarios while the abundance and diversity of the bacterial community declined with the amplification of CO2 leakage quantity and leakage time, and some bacteria species became the dominant bacteria species in the bacteria community, therefore the increase of Acidobacteria species would be a biological indicator for the impact of sealed CO2 leakage on soil ecology system.
authors.affiliation: College of Territorial Resources and Tourism, Anhui Normal University, Wuhu 241003, China. tdwy22@sina.com
authors.forename: Di
authors.initials: D
authors.lastname: Tian
authors.affiliation: Key Laboratory of Agro-Environment and Climate Change, Ministry of Agriculture, Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Ma
authors.affiliation: Key Laboratory of Agro-Environment and Climate Change, Ministry of Agriculture, Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Yu-E
authors.initials: YE
authors.lastname: Li
authors.affiliation: College of Territorial Resources and Tourism, Anhui Normal University, Wuhu 241003, China.
authors.forename: Liang-Song
authors.initials: LS
authors.lastname: Zha
authors.affiliation: China Meteorological Administration Engineering Consulting Center, Beijing 100081, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Key Laboratory of Agro-Environment and Climate Change, Ministry of Agriculture, Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Zou
authors.affiliation: Key Laboratory of Agro-Environment and Climate Change, Ministry of Agriculture, Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liu
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics; D002245:Carbon Dioxide / Q000737:chemistry*; D004269:DNA, Bacterial / Q000235:genetics; D017753:Ecosystem; D059014:High-Throughput Nucleotide Sequencing; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012987:Soil; D002245:Carbon Dioxide