title: Induction of monocyte-to-dendritic cell maturation by extracorporeal photochemotherapy: initiation via direct platelet signaling.
abstract: Extracorporeal Photochemotherapy (ECP) is a widely used therapy for cutaneous T cell lymphoma (CTCL). Although the mechanism of clinical action of ECP is not precisely established, previous studies have shown evidence of induction of dendritic cells (DCs). Here we show that, under flow conditions similar to those in post-capillary venules, ECP promotes platelet immobilization and activation, initiating stepwise receptor-ligand interactions with monocytes, which then differentiate into DC. These findings clarify how ECP directly stimulates DC maturation; suggest a new clinically applicable approach to the obtainment of DC; and identify a novel mechanism that may reflect physiological induction of DC.
authors.affiliation: Department of Dermatology, Yale University, School of Medicine, New Haven, CT, USA.
authors.forename: Tyler S
authors.initials: TS
authors.lastname: Durazzo
authors.affiliation: Department of Dermatology, Yale University, School of Medicine, New Haven, CT, USA; Comprehensive Cancer Center of Yale University, School of Medicine, New Haven, CT, USA.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Tigelaar
authors.affiliation: Department of Dermatology, Yale University, School of Medicine, New Haven, CT, USA.
authors.forename: Renata
authors.initials: R
authors.lastname: Filler
authors.affiliation: Kings College, London, UK.
authors.forename: Adrian
authors.initials: A
authors.lastname: Hayday
authors.affiliation: Department of Dermatology, Yale University, School of Medicine, New Haven, CT, USA; Comprehensive Cancer Center of Yale University, School of Medicine, New Haven, CT, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Girardi
authors.affiliation: Department of Dermatology, Yale University, School of Medicine, New Haven, CT, USA; Comprehensive Cancer Center of Yale University, School of Medicine, New Haven, CT, USA. Electronic address: redelson@yale.edu.
authors.forename: Richard L
authors.initials: RL
authors.lastname: Edelson
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D009000:Monocytes / Q000276:immunology*; D017893:Photopheresis; D015539:Platelet Activation / Q000187:drug effects / Q000528:radiation effects; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects
keywords: Dendritic cells; Extracorporeal photochemotherapy; Monocytes; P-selectin; Platelets