title: Neuroprotective effect of buyang huanwu decoction on rat ischemic/reperfusion brain damage by promoting migration of neural precursor cells.
abstract: Buyang Huanwu Decoction (BYHWD) is a classic formula widely used for treating stroke-induced disability, the highest morbidity of neurological disorders in China. However, the mechanism of its neuroprotection has not been fully clarified. Previous reports indicated that BYHWD may promote growth and differentiation of neural precursor cells (NPCs). The present study focused on the effects of BYHWD on migration of NPCs in rats with middle cerebral artery occlusion (MCAO). Rats were treated with different doses of BYHWD (12 and 24 grams/kg) from day 1 to day 21 after model building. BYHWD could increase the survival rate and decrease neurological scores and infarct volume as compared with the vehicle-treated MCAO rats. Moreover, BYHWD treatment significantly increased 5-bromo-2-deoxyuridine (BrdU)-positive cells in the subventricular zone (SVZ), subgranular zone (SGZ), and corpus striatum (CS) of the infarct brain. Interestingly, BYHWD could markedly enhance BrdU(+)/doublecortin(+) cells not only in the SVZ and SGZ but also in CS, by up-regulating the protein expression of migration activators, including stromal cell derived factor-1, CXC chemokine receptor 4, vascular endothelial growth factor, Reelin, and brain-derived neurotrophic factor in the ipsilateral infarct area after MCAO. In addition, BYHWD treatment was able to promote the neuronal differentiation, which was closely related to the migratory process of NPCs in MCAO rats. These findings offer evidence for the first time that BYHWD may exert its neuroprotective effects partially by promotion of NPCs migration to ischemic brain areas.
authors.affiliation: 1 Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences , Beijing, China .
authors.forename: Xiangying
authors.initials: X
authors.lastname: Kong
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Su
authors.forename: Jia
authors.initials: J
authors.lastname: Zhu
authors.forename: Jianzhu
authors.initials: J
authors.lastname: Wang
authors.forename: Hongye
authors.initials: H
authors.lastname: Wan
authors.forename: Micun
authors.initials: M
authors.lastname: Zhong
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Na
authors.initials: N
authors.lastname: Lin
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000473:pathology; D002465:Cell Movement / Q000187:drug effects; D000089662:Doublecortin Protein; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000091042:Reelin Protein; D015427:Reperfusion Injury / Q000517:prevention & control*
chemical_list: C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D000091042:Reelin Protein; C000717129:Reln protein, rat; C460102:buyang huanwu