title: Profiling of components and validated determination of iridoids in Gardenia Jasminoides Ellis fruit by a high-performance-thin-layer- chromatography/mass spectrometry approach.
abstract: A novel method was set up with the aim to obtain a simultaneous cross comparative evaluation of different Gardenia Jasminoides Ellis fruits by the HPTLC fingerprint approach. The main components among the iridoid, hydroxycinnamic derivative and crocin classes were identified by TLC-MS ancillary techniques. The iridoids geniposide, gardenoside and genepin-1-β-d-gentiobioside were also quantitated by densitometric scanning at 240nm. LiChrospher HPTLC Silica gel 60 RP-18 W F254, 20cm×10cm plates with acetonitrile: formic acid 0.1% (40:60 v/v) as the mobile phase was used. The method was validated giving rise to a dependable and high throughput procedure well suited to routine applications. Iridoids were quantified in the range of 240-1140ng with RSD of repeatability and intermediate precision between 0.9-2.5% and accuracy with bias 1.6-2.6%. The method was tested on six commercial Gardenia Jasminoides fruit samples.
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università di Firenze, Via Ugo Schiff 6, Sesto Fiorentino, I-50019 Firenze, Italy. Electronic address: coran@unifi.it.
authors.forename: Silvia A
authors.initials: SA
authors.lastname: Coran
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università di Firenze, Via Ugo Schiff 6, Sesto Fiorentino, I-50019 Firenze, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Mulas
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università di Firenze, Via Ugo Schiff 6, Sesto Fiorentino, I-50019 Firenze, Italy.
authors.forename: Alessio
authors.initials: A
authors.lastname: Vasconi
mesh_terms: D002855:Chromatography, Thin Layer / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D032062:Gardenia / Q000737:chemistry*; D039823:Iridoids / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D039823:Iridoids; C007835:geniposide; C056587:gardenoside
keywords: Caffeoylquinic acid derivatives; Crocins; Gardenia Jasminoides (Zhi-zi); HPTLC fingerprint; HPTLC-densitometry; Iridoids; TLC–MS