title: Benzene containing polyhydroxyalkanoates homo- and copolymers synthesized by genome edited Pseudomonas entomophila.
abstract: Microbial synthesis of functional polymers has become increasingly important for industrial biotechnology. For the first time, it became possible to synthesize controllable composition of poly(3-hydroxyalkanoate) (P3HA) consisting of 3-hydroxydodecanoate (3HDD) and phenyl group on the side-chain when chromosome of Pseudomonas entomophila was edited to weaken its β-oxidation. Cultured in the presence of 5-phenylvaleric acid (PVA), the edited P. entomophila produced only homopolymer poly(3-hydroxy-5-phenylvalerate) or P(3HPhV). While copolyesters P(3HPhV-co-3HDD) of 3-hydroxy-5-phenylvalerate (3HPhV) and 3-hydroxydodecanoate (3HDD) were synthesized when the strain was grown on mixtures of PVA and dodecanoic acid (DDA). Compositions of 3HPhV in P(3HPhV-co-3HDD) were controllable ranging from 3% to 32% depending on DDDA/PVA ratios. Nuclear magnetic resonance (NMR) spectra clearly indicated that the polymers were homopolymer of P(3HPhV) and random copolymers of 3HPhV and 3HDD. Their mechanical and thermal properties varied dramatically depending on the monomer ratios. Our results demonstrated the possibility to produce tailor-made, novel functional PHA using the chromosome edited P. entomophila.
authors.affiliation: MOE Key Lab of Bioinformatics, Department of Biological Science and Biotechnology, School of Life Science, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, 100084, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Shen
authors.forename: Longwei
authors.initials: L
authors.lastname: Cai
authors.forename: Dechuan
authors.initials: D
authors.lastname: Meng
authors.forename: Linping
authors.initials: L
authors.lastname: Wu
authors.forename: Kai
authors.initials: K
authors.lastname: Guo
authors.forename: Guoxing
authors.initials: G
authors.lastname: Dong
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Jinchun
authors.initials: J
authors.lastname: Chen
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Chen
mesh_terms: D001554:Benzene / Q000032:analysis*; D002876:Chromosomes, Bacterial; D003470:Culture Media; D016680:Genome, Bacterial; D019906:Nuclear Magnetic Resonance, Biomolecular; D054813:Polyhydroxyalkanoates / Q000737:chemistry* / Q000378:metabolism; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism*
chemical_list: D003470:Culture Media; D054813:Polyhydroxyalkanoates; D001554:Benzene