title: Novel microarrays for simultaneous serodiagnosis of multiple antiviral antibodies.
abstract: We developed an automated diagnostic system for the detection of virus-specific immunoglobulin Gs (IgGs) that was based on a microarray platform. We compared efficacies of our automated system with conventional enzyme immunoassays (EIAs). Viruses were immobilized to microarrays using a radical cross-linking reaction that was induced by photo-irradiation. A new photoreactive polymer containing perfluorophenyl azide (PFPA) and poly(ethylene glycol) methacrylate was prepared and coated on plates. Inactivated measles, rubella, mumps, Varicella-Zoster and recombinant Epstein-Barr viruse antigen were added to coated plates, and irradiated with ultraviolet light to facilitate immobilization. Virus-specific IgGs in healthy human sera were assayed using these prepared microarrays and the results obtained compared with those from conventional EIAs. We observed high correlation (0.79-0.96) in the results between the automated microarray technique and EIAs. The microarray-based assay was more rapid, involved less reagents and sample, and was easier to conduct compared with conventional EIA techniques. The automated microarray system was further improved by introducing reagent storage reservoirs inside the chamber, thereby conserving the use of expensive reagents and antibodies. We considered the microarray format to be suitable for rapid and multiple serological diagnoses of viral diseases that could be developed further for clinical applications.
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan.
authors.forename: Ponnurengam Malliappan
authors.initials: PM
authors.lastname: Sivakumar
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Moritsugu
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan.
authors.forename: Sei
authors.initials: S
authors.lastname: Obuse
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Isoshima
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan ; Consonal Biotechnologies Co., Ltd., Funabashi, Chiba, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Tashiro
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan ; Consonal Biotechnologies Co., Ltd., Funabashi, Chiba, Japan ; Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, Wako, Saitama, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D001386:Azides; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D006845:Hydrocarbons, Fluorinated; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000097:blood; D009109:Mumps virus / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis; D012412:Rubella virus / Q000276:immunology; D012698:Serologic Tests / Q000379:methods*
chemical_list: D000914:Antibodies, Viral; D001386:Azides; D006845:Hydrocarbons, Fluorinated; D007074:Immunoglobulin G; C556136:perfluorophenyl azide