title: [Antibody preparation and enzyme linked immunosorbent assay of Lonicera japonica phenylalnine ammonia lyase].
abstract: The expression of phenylalnine ammonia lyase (LJPAL1) is closely related to the content of active compounds in Lonicera japonica. In this paper, a prokaryotic expression vector is constructed and LJPAL1 protein is expressed in E. coli. Three antigen sites were synthesized to peptide antigen and prepared polyclonal antibody of Anti-LJT-1, Anti-LJT-2 and Anti-LJT-3, separately. Antibody Anti-LJT-2 was screened using Western blotting. And indirect ELISA was built using Anti-LJT-2. The results of this study will be a base for honeysuckle chemical quality and evaluation kits.
authors.affiliation: National Resource Center for Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Zhou-yong
authors.initials: ZY
authors.lastname: Wang
authors.affiliation: National Resource Center for Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Lu-qi
authors.initials: LQ
authors.lastname: Huang
authors.affiliation: National Resource Center for Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: China Agriculture University, Beijing 100094, China.
authors.forename: Bao-min
authors.initials: BM
authors.lastname: Wang
mesh_terms: D000906:Antibodies / Q000276:immunology* / Q000378:metabolism; D000918:Antibody Specificity; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D035264:Flowers / Q000737:chemistry; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors; D031239:Lonicera / Q000737:chemistry*; D010650:Phenylalanine Ammonia-Lyase / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry; D010957:Plasmids; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D000906:Antibodies; D010650:Phenylalanine Ammonia-Lyase