title: Biofilm cultivation of the oleaginous microalgae Pseudochlorococcum sp.
abstract: The cultivation of microalgae in biofilm has been a potential way to overcome the shortcoming of conventional algal culture modes of open pond and photobioreactors in liquid suspension. However, the growth characteristics and related effect factors of the biofilm are still far from being understood. In this work, oleaginous microalgae species Pseudochlorococcum was cultured in an attached biofilm and influential factors on the growth rate of biofilm were investigated. The results showed that Pseudochlorococcum sp. preferred to accumulate more biomass on hydrophilic substrata than on hydrophobic one. The photon flux density of 100 μmol m(-2 )s(-1) was its light saturation point. The optimal inoculum density was about 3-5 g m(-2). The appropriate concentrations of nitrogen, phosphorus in medium and CO(2) in aerated gas were determined as 8.8, 0.22 mmol L(-1) and 1 %, respectively.
authors.affiliation: Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Science, Qingdao, 266101, Shandong, People's Republic of China.
authors.forename: Bei
authors.initials: B
authors.lastname: Ji
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Ningning
authors.initials: N
authors.lastname: Zhang
authors.forename: Junfeng
authors.initials: J
authors.lastname: Wang
authors.forename: Giovanni Antonio
authors.initials: GA
authors.lastname: Lutzu
authors.forename: Tianzhong
authors.initials: T
authors.lastname: Liu
mesh_terms: D018441:Biofilms; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002245:Carbon Dioxide / Q000737:chemistry; D002708:Chlorella / Q000254:growth & development*; D057927:Hydrophobic and Hydrophilic Interactions; D008027:Light; D058086:Microalgae / Q000254:growth & development*; D009584:Nitrogen / Q000737:chemistry; D010758:Phosphorus / Q000737:chemistry; D058433:Photobioreactors; D017785:Photons; D061005:Ponds; D013997:Time Factors
chemical_list: D002245:Carbon Dioxide; D010758:Phosphorus; D009584:Nitrogen