title: Quantitative cerebral blood flow imaging with extended-focus optical coherence microscopy.
abstract: Quantitative three-dimensional blood flow imaging is a valuable technique to investigate the physiology of the brain. Two-photon microscopy (2PM) allows quantification of the local blood flow velocity with micrometric resolution by performing repeated line scans, but prohibitively long measurement times would be required to apply this technique to full three-dimensional volumes. By multiplexing the image acquisition over depth, Fourier domain optical coherence tomography (FDOCT) enables quantification of blood flow velocities with a high volume acquisition rate, albeit at a relatively low spatial resolution. Extended-focus optical coherence microscopy (xfOCM) increases the lateral resolution without sacrificing depth of field and therefore combines the high volume acquisition rate of FDOCT with a resolution comparable to 2PM. Here, we demonstrate high-resolution quantitative imaging of the blood flow velocity vector's magnitude in the adult murine brain with xfOCM.
authors.forename: Arno
authors.initials: A
authors.lastname: Bouwens
authors.forename: Tristan
authors.initials: T
authors.lastname: Bolmont
authors.forename: Daniel
authors.initials: D
authors.lastname: Szlag
authors.forename: Corinne
authors.initials: C
authors.lastname: Berclaz
authors.forename: Theo
authors.initials: T
authors.lastname: Lasser
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply; D002560:Cerebrovascular Circulation; D051379:Mice; D041623:Tomography, Optical Coherence / Q000379:methods*