title: Large-scale, high-density (up to 512 channels) recording of local circuits in behaving animals.
abstract: Monitoring representative fractions of neurons from multiple brain circuits in behaving animals is necessary for understanding neuronal computation. Here, we describe a system that allows high-channel-count recordings from a small volume of neuronal tissue using a lightweight signal multiplexing headstage that permits free behavior of small rodents. The system integrates multishank, high-density recording silicon probes, ultraflexible interconnects, and a miniaturized microdrive. These improvements allowed for simultaneous recordings of local field potentials and unit activity from hundreds of sites without confining free movements of the animal. The advantages of large-scale recordings are illustrated by determining the electroanatomic boundaries of layers and regions in the hippocampus and neocortex and constructing a circuit diagram of functional connections among neurons in real anatomic space. These methods will allow the investigation of circuit operations and behavior-dependent interregional interactions for testing hypotheses of neural networks and brain function.
authors.affiliation: New York University Neuroscience Institute, School of Medicine, New York University, New York, New York;
authors.forename: Antal
authors.initials: A
authors.lastname: Berényi
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Somogyvári
authors.forename: Anett J
authors.initials: AJ
authors.lastname: Nagy
authors.forename: Lisa
authors.initials: L
authors.lastname: Roux
authors.forename: John D
authors.initials: JD
authors.lastname: Long
authors.forename: Shigeyoshi
authors.initials: S
authors.lastname: Fujisawa
authors.forename: Eran
authors.initials: E
authors.lastname: Stark
authors.forename: Anthony
authors.initials: A
authors.lastname: Leonardo
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Harris
authors.forename: György
authors.initials: G
authors.lastname: Buzsáki
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D002540:Cerebral Cortex / Q000502:physiology* / Q000601:surgery; D004567:Electrodes, Implanted; D008297:Male; D051379:Mice; D009415:Nerve Net / Q000502:physiology* / Q000601:surgery; D009474:Neurons / Q000502:physiology*; D051381:Rats; D020318:Rats, Long-Evans; D012815:Signal Processing, Computer-Assisted; D012984:Software
keywords: behaving rats and mice; local field potential; monosynaptic connections; unit firing