title: A new fluorescent pH probe for imaging lysosomes in living cells.
abstract: A new rhodamine B-based pH fluorescent probe has been synthesized and characterized. The probe responds to acidic pH with short response time, high selectivity and sensitivity, and exhibits a more than 20-fold increase in fluorescence intensity within the pH range of 7.5-4.1 with the pKa value of 5.72, which is valuable to study acidic organelles in living cells. Also, it has been successfully applied to HeLa cells, for its low cytotoxicity, brilliant photostability, good membrane permeability and no 'alkalizing effect' on lysosomes. The results demonstrate that this probe is a lysosome-specific probe, which can selectively stain lysosomes and monitor lysosomal pH changes in living cells.
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors.forename: Hong-Shui
authors.initials: HS
authors.lastname: Lv
authors.affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, PR China.
authors.forename: Shu-Ya
authors.initials: SY
authors.lastname: Huang
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors.forename: Xi
authors.initials: X
authors.lastname: Dai
authors.affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, PR China. Electronic address: miaojy@sdu.edu.cn.
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Miao
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China. Electronic address: bxzhao@sdu.edu.cn.
authors.forename: Bao-Xiang
authors.initials: BX
authors.lastname: Zhao
mesh_terms: D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000737:chemistry / Q000378:metabolism*; D012235:Rhodamines / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; C029773:rhodamine B
keywords: Fluorescent probe; Lysosomes imaging; Rhodamine B; pH chemodosimeter