title: Refractometric total protein concentrations in icteric serum from dogs.
abstract: OBJECTIVE
  To determine whether high serum bilirubin concentrations interfere with the measurement of serum total protein concentration by refractometry and to assess potential biases among refractometer measurements.
  
  
  METHODS
  Evaluation study.
  
  
  METHODS
  Sera from 2 healthy Greyhounds.
  
  
  METHODS
  Bilirubin was dissolved in 0.1M NaOH, and the resulting solution was mixed with sera from 2 dogs from which food had been withheld to achieve various bilirubin concentrations up to 40 mg/dL. Refractometric total protein concentrations were estimated with 3 clinical refractometers. A biochemical analyzer was used to measure biuret assay-based total protein and bilirubin concentrations with spectrophotometric assays.
  
  
  RESULTS
  No interference with refractometric measurement of total protein concentrations was detected with bilirubin concentrations up to 41.5 mg/dL. Biases in refractometric total protein concentrations were detected and were related to the conversion of refractive index values to total protein concentrations.
  
  
  CONCLUSIONS
  Hyperbilirubinemia did not interfere with the refractometric estimation of serum total protein concentration. The agreement among total protein concentrations estimated by 3 refractometers was dependent on the method of conversion of refractive index to total protein concentration and was independent of hyperbilirubinemia.
authors.affiliation: Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506.
authors.forename: Aradhana
authors.initials: A
authors.lastname: Gupta
authors.forename: Steven L
authors.initials: SL
authors.lastname: Stockham
mesh_terms: D000818:Animals; D001663:Bilirubin / Q000737:chemistry* / Q000378:metabolism; D001798:Blood Proteins / Q000737:chemistry* / Q000378:metabolism; D004283:Dog Diseases / Q000097:blood*; D004285:Dogs; D006932:Hyperbilirubinemia / Q000097:blood / Q000662:veterinary*; D012031:Refractometry / Q000379:methods / Q000662:veterinary*
chemical_list: D001798:Blood Proteins; D001663:Bilirubin