title: RNAi-mediated knockdown of FANCF suppresses cell proliferation, migration, invasion, and drug resistance potential of breast cancer cells.
abstract: Fanconi anemia complementation group F protein (FANCF) is a key factor, which maintains the function of FA/BRCA, a DNA damage response pathway. However, the functional role of FANCF in breast cancer has not been elucidated. We performed a specific FANCF-shRNA knockdown of endogenous FANCF in vitro. Cell viability was measured with a CCK-8 assay. DNA damage was assessed with an alkaline comet assay. Apoptosis, cell cycle, and drug accumulation were measured by flow cytometry. The expression levels of protein were determined by Western blot using specific antibodies. Based on these results, we used cell migration and invasion assays to demonstrate a crucial role for FANCF in those processes. FANCF shRNA effectively inhibited expression of FANCF. We found that proliferation of FANCF knockdown breast cancer cells (MCF-7 and MDA-MB-435S) was significantly inhibited, with cell cycle arrest in the S phase, induction of apoptosis, and DNA fragmentation. Inhibition of FANCF also resulted in decreased cell migration and invasion. In addition, FANCF knockdown enhanced sensitivity to doxorubicin in breast cancer cells. These results suggest that FANCF may be a potential target for molecular, therapeutic intervention in breast cancer.
authors.affiliation: China Medical University, School of Pharmacy, Department of Pharmacology, Heping Ward, Shenyang CityLiaoning, China, Department of Pharmacology, School of Pharmacy, China Medical University, Heping Ward, Shenyang City, Liaoning, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhao
authors.affiliation: China Medical University, School of Pharmacy, Department of Pharmacology, Heping Ward, Shenyang CityLiaoning, China, Department of Pharmacology, School of Pharmacy, China Medical University, Heping Ward, Shenyang City, Liaoning, China.
authors.forename: N
authors.initials: N
authors.lastname: Li
authors.affiliation: China Medical University, School of Pharmacy, Department of Pharmacology, Heping Ward, Shenyang CityLiaoning, China, Department of Pharmacology, School of Pharmacy, China Medical University, Heping Ward, Shenyang City, Liaoning, China.
authors.forename: J K
authors.initials: JK
authors.lastname: Yu
authors.affiliation: China Medical University, School of Pharmacy, Department of Pharmacology, Heping Ward, Shenyang CityLiaoning, China, Department of Pharmacology, School of Pharmacy, China Medical University, Heping Ward, Shenyang City, Liaoning, China.
authors.forename: H T
authors.initials: HT
authors.lastname: Tang
authors.affiliation: China Medical University, School of Pharmacy, Department of Pharmacology, Heping Ward, Shenyang CityLiaoning, China, Department of Pharmacology, School of Pharmacy, China Medical University, Heping Ward, Shenyang City, Liaoning, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Li
authors.affiliation: China Medical University, School of Pharmacy, Department of Pharmacology, Heping Ward, Shenyang CityLiaoning, China, Department of Pharmacology, School of Pharmacy, China Medical University, Heping Ward, Shenyang City, Liaoning, China.
authors.forename: M
authors.initials: M
authors.lastname: He
authors.affiliation: China Medical University, School of Pharmacy, Department of Pharmacology, Heping Ward, Shenyang CityLiaoning, China, Department of Pharmacology, School of Pharmacy, China Medical University, Heping Ward, Shenyang City, Liaoning, China.
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Yu
authors.affiliation: China Medical University, School of Pharmacy, Department of Pharmacology, Heping Ward, Shenyang CityLiaoning, China, Department of Pharmacology, School of Pharmacy, China Medical University, Heping Ward, Shenyang City, Liaoning, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Bai
authors.affiliation: China Medical University, Institute of Pathology and Pathophysiology, Heping Ward, Shenyang City,Liaoning, China, Institute of Pathology and Pathophysiology, China Medical University, Heping Ward, Shenyang City, Liaoning, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Zheng
authors.affiliation: China Medical University, Institute of Pathology and Pathophysiology, Heping Ward, Shenyang City,Liaoning, China, Institute of Pathology and Pathophysiology, China Medical University, Heping Ward, Shenyang City, Liaoning, China.
authors.forename: E H
authors.initials: EH
authors.lastname: Wang
authors.affiliation: China Medical University, School of Pharmacy, Department of Pharmacology, Heping Ward, Shenyang CityLiaoning, China, Department of Pharmacology, School of Pharmacy, China Medical University, Heping Ward, Shenyang City, Liaoning, China.
authors.forename: M J
authors.initials: MJ
authors.lastname: Wei
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics*; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics*; D004351:Drug Resistance; D004354:Drug Screening Assays, Antitumor; D052236:Fanconi Anemia Complementation Group D2 Protein / Q000235:genetics*; D052219:Fanconi Anemia Complementation Group F Protein / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering
chemical_list: D000970:Antineoplastic Agents; C499905:FANCD2 protein, human; C499901:FANCF protein, human; D052236:Fanconi Anemia Complementation Group D2 Protein; D052219:Fanconi Anemia Complementation Group F Protein; D034741:RNA, Small Interfering