title: Depolymerization of sulfated polysaccharides under hydrothermal conditions.
abstract: Fucoidan and chondroitin sulfate, which are well known sulfated polysaccharides, were depolymerized under hydrothermal conditions (120-180°C, 5-60min) as a method for the preparation of sulfated polysaccharides with controlled molecular weights. Fucoidan was easily depolymerized, and the change of the molecular weight values depended on the reaction temperature and time. The degree of sulfation and IR spectra of the depolymerized fucoidan did not change compared with those of untreated fucoidan at reaction temperatures below 140°C. However, fucoidan was partially degraded during depolymerization above 160°C. Nearly the same depolymerization was observed for chondroitin sulfate. These results indicate that hydrothermal treatment is applicable for the depolymerization of sulfated polysaccharides, and that low molecular weight products without desulfation and deformation of the initial glycan structures can be obtained under mild hydrothermal conditions.
authors.affiliation: Division of Instrumental Analysis, Research Center for Bioscience and Technology, Tottori University, Tottori 680-8550, Japan. Electronic address: morimoto@chem.tottori-u.ac.jp.
authors.forename: Minoru
authors.initials: M
authors.lastname: Morimoto
authors.affiliation: Division of Applied Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Takatori
authors.affiliation: Division of Applied Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Hayashi
authors.affiliation: Division of Applied Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan.
authors.forename: Daiki
authors.initials: D
authors.lastname: Mori
authors.affiliation: Scientific Crime Laboratory, Tottori Prefectural Police Headquarters, Tottori 680-0911, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Takashima
authors.affiliation: Tottori Institute of Industrial Technology, Tottori 689-1112, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Yoshida
authors.affiliation: Koyo Chemical Co. Ltd, Kita-Ku, Osaka 530-0051, Japan.
authors.forename: Kimihiko
authors.initials: K
authors.lastname: Sato
authors.affiliation: Marine Products Kimuraya Co. Ltd, Sakaiminato 684-0072, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kawamoto
authors.affiliation: Department of Regional Environment, Faculty of Regional Sciences, Tottori University, Tottori 680-8551, Japan.
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Tamura
authors.affiliation: Division of Applied Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan.
authors.forename: Hironori
authors.initials: H
authors.lastname: Izawa
authors.affiliation: Division of Applied Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan.
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Ifuku
authors.affiliation: Division of Applied Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Saimoto
mesh_terms: D002809:Chondroitin Sulfates / Q000737:chemistry*; D006358:Hot Temperature; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D011134:Polysaccharides; D002809:Chondroitin Sulfates; C007789:fucoidan
keywords: Chondroitin sulfate; Depolymerization without desulfation; Fucoidan; Hydrothermal treatment; Sulfated polysaccharide