title: MiR-193b and miR-365-1 are not required for the development and function of brown fat in the mouse.
abstract: Generating heat and maintaining body temperature is the primary function of brown adipose tissue (BAT). Previous studies have implicated microRNAs, including miR-193b and miR-365-1, in BAT differentiation. We used mouse genetics to further understand the specific contributions of these two miRs. BAT function in mice with an inactivated miR-193b-365-1 locus, as determined by their response to the selective β3 adrenergic receptor agonist CL316.243 and their tolerance to cold exposure, was normal and expression of genes associated with functional BAT, including Prdm16 and Ucp1, was unaffected. In addition, genome-wide expression profiles of miRNAs and mRNAs in BAT in the presence and absence of miR-193b-365-1 were determined. In summary, these data demonstrate, in contrast to earlier work, that the development, differentiation, and function of BAT do not require the presence of miR-193b and miR-365-1.
authors.affiliation: Laboratory of Genetics and Physiology; NIDDK, NIH; Bethesda, MD USA.
authors.forename: Yonatan
authors.initials: Y
authors.lastname: Feuermann
authors.affiliation: Laboratory of Genetics and Physiology; NIDDK, NIH; Bethesda, MD USA.
authors.forename: Keunsoo
authors.initials: K
authors.lastname: Kang
authors.affiliation: Mouse Metabolic Core; NIDDK, NIH; Bethesda, MD USA.
authors.forename: Oksana
authors.initials: O
authors.lastname: Gavrilova
authors.affiliation: Laboratory of Genetics and Physiology; NIDDK, NIH; Bethesda, MD USA; Georg-Speyer-Haus, Institute for Tumor Biology and Experimental Therapy and LOEWE Center for Cell and Gene Therapy Frankfurt and Department of Hematology/Oncology; Goethe University; Frankfurt am Main, Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Haetscher
authors.affiliation: Laboratory of Genetics and Physiology; NIDDK, NIH; Bethesda, MD USA.
authors.forename: Seung Jin
authors.initials: SJ
authors.lastname: Jang
authors.affiliation: Laboratory of Metabolism, Center for Cancer Research, NCI; NIH; Bethesda, MD USA.
authors.forename: Kyung Hyun
authors.initials: KH
authors.lastname: Yoo
authors.affiliation: Laboratory of Metabolism, Center for Cancer Research, NCI; NIH; Bethesda, MD USA.
authors.forename: Changtao
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Laboratory of Genetics and Physiology; NIDDK, NIH; Bethesda, MD USA.
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Gonzalez
authors.affiliation: Laboratory of Genetics and Physiology; NIDDK, NIH; Bethesda, MD USA.
authors.forename: Gertraud W
authors.initials: GW
authors.lastname: Robinson
authors.affiliation: Laboratory of Genetics and Physiology; NIDDK, NIH; Bethesda, MD USA.
authors.forename: Lothar
authors.initials: L
authors.lastname: Hennighausen
mesh_terms: D002001:Adipose Tissue, Brown / Q000254:growth & development* / Q000502:physiology*; D058667:Adrenergic beta-3 Receptor Agonists / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004149:Dioxoles / Q000494:pharmacology; D005786:Gene Expression Regulation; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000502:physiology*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000071256:Uncoupling Protein 1
chemical_list: D058667:Adrenergic beta-3 Receptor Agonists; D004268:DNA-Binding Proteins; D004149:Dioxoles; D007473:Ion Channels; C568257:MIRN193 microRNA, mouse; C000589043:MIRN365 microRNA, mouse; D035683:MicroRNAs; D024101:Mitochondrial Proteins; C521133:Prdm16 protein, mouse; D014157:Transcription Factors; C000604372:Ucp1 protein, mouse; D000071256:Uncoupling Protein 1; C076126:disodium (R,R)-5-(2-((2-(3-chlorophenyl)-2-hydroxyethyl)-amino)propyl)-1,3-benzodioxole-2,3-dicarboxylate