title: Methicillin susceptible Staphylococcus aureus (MSSA) of clonal complex CC398, t571 from infections in humans are still rare in Germany.
abstract: Methicillin-susceptible Staphylococcus aureus (MSSA) attributed to clonal complex (CC) 398 and exhibiting spa-type t571 received attention in Europe and in the USA for being associated with severe infections in humans. As this spa-type is exhibited by livestock-associated (LA) Methicillin-resistant S. aureus (MRSA) as well, it is important to discriminate LA- and human-derived strains by easy to perform, PCR-based methods. MSSA t571 contain phage int3 carrying scn and chp, whereas LA-MRSA t571 lack these markers. In contrast, pathogenicity island SaPIbov5 (detected by PCR bridging vwbbov and scn) is contained by LA-MRSA t571 and absent in the human MSSA subpopulation. Furthermore, MSSA t571 contain erm(T), the particular genomic arrangement of which was assessed by a PCR bridging erm(T) and the adjacent transposase gene. MSSA t571 are rare so far in Germany among isolates from infections in humans (0.14%) as well as among isolates from nasal colonization (0.13%). LA-MRSA t571 are also infrequent among MRSA isolated from carriage at admission to hospitals (0.1%) and also among isolates from infections in humans (0.013%).
authors.affiliation: National Reference Center for Staphylococci and Enterococci, Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany.
authors.forename: Christiane
authors.initials: C
authors.lastname: Cuny
authors.affiliation: National Reference Center for Staphylococci and Enterococci, Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany.
authors.forename: Franziska
authors.initials: F
authors.lastname: Layer
authors.affiliation: Institute of Hygiene, University Hospital Münster, Münster, Germany.
authors.forename: Robin
authors.initials: R
authors.lastname: Köck
authors.affiliation: National Reference Center for Staphylococci and Enterococci, Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany.
authors.forename: Guido
authors.initials: G
authors.lastname: Werner
authors.affiliation: Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Witte
mesh_terms: D000818:Animals; D005819:Genetic Markers / Q000235:genetics; D005858:Germany / Q000453:epidemiology; D006736:Horses / Q000382:microbiology; D006801:Humans; D058751:Livestock / Q000382:microbiology; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D009666:Nose / Q000382:microbiology; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology*
chemical_list: D005819:Genetic Markers