title: [Preparation and immunological evaluation of oral solution of egg yolk-derived hepatitis B virus-specific transfer factor].
abstract: OBJECTIVE
  To prepare the oral solution of egg yolk hepatitis B virus (HBV)-specific transfer factor (EYHBV-TF) and evaluate its immunological activity as an immune regulator against hepatitis B.
  
  
  METHODS
  From hens immunized with the Hepatitis B vaccine the egg yolk was isolated to extract the specific transfer factor EYHBV-TF, and its physicochemical properties were examined. Leukocyte adhesion inhibition test (LAI) was performed to detect the immunogenic activity of EYHBV-TF. The solution of EYHBV-TF was then administered orally in normal mice, and the specific cellular immune activity induced was assayed with delayed type skin hypersensitivity test (DTH), with the non-specific immune activity assessed with immune organ index. The immune responses induced by oral EYHBV-STF solution were compared with those by EYHBV-STF injection and by different dosages (injection and oral) of porcine spleen HBV-specific transfer factor (PSHBV-STF), porcine spleen nonspecific transfer factor, and egg yolk extracts from non-immunized hens.
  
  
  RESULTS
  The prepared EYHBV-STF oral solution, which met the standards for biological products, could inhibit leukocyte adhesion in vitro and significantly enhance mouse foot pad swelling, demonstrating its capability of transferring antigen-specific delayed type hypersensitivity reactions to naive recipient. EYHBV-STF oral solution also significantly improved the immune organ index in mice (P<0 01) with similar effects to those caused by EYHBV-STF injections and by PSHBV-STF injection and oral solution.
  
  
  CONCLUSIONS
  Orally administered EYHBV-STF and EYHBV-STF injection both possess hepatitis B antigen-specific cellular immune activity and can significantly enhance specific cellular immune responses.
authors.affiliation: Jiangxi Academy of Medical Sciences, Nanchang 330006, China. E-mail: ypxu430@163.com.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Xu
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.forename: Xuejun
authors.initials: X
authors.lastname: Zhan
authors.forename: Daze
authors.initials: D
authors.lastname: Xie
authors.forename: Ge
authors.initials: G
authors.lastname: Dai
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
mesh_terms: D000818:Animals; D002645:Chickens; D004530:Egg Yolk / Q000737:chemistry; D006509:Hepatitis B / Q000188:drug therapy*; D006511:Hepatitis B Antigens; D006515:Hepatitis B virus / Q000187:drug effects*; D007111:Immunity, Cellular; D007114:Immunization; D051379:Mice; D013552:Swine; D014165:Transfer Factor / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D006511:Hepatitis B Antigens; D014165:Transfer Factor