title: The potential role of Azadirachta indica treatment on cisplatin-induced hepatotoxicity and oxidative stress in female rats.
abstract: Azadirachta indica A. Juss. (neem, family: Meliaceae) is perhaps the most commonly used traditional medicinal plant of India. In this study we investigated the protective effect of methanolic neem leaves extract (MNLE; 500 mg/Kg bwt) on rats treated with cisplatin (CDDP)-induced hepatotoxicity. Adult rats were randomly divided into four groups. CDDP was given to rats by intraperitoneal injection, while MNLE was given by oral gavage for 5 days after the CDDP injection. The injury and oxidative stress caused by CDDP on the liver and the effect of MNLE were evaluated by measuring (a) histological changes, (b) tissue biochemical oxidant and antioxidant parameters, and (c) investigating apoptosis markers immunohistochemically and by real time PCR. After treatment with MNLE, the histological damage and apoptosis induction caused by cisplatin were improved. Malondialdehyde and nitric oxide were significantly decreased; the antioxidant system, namely, glutathione content, glutathione-S-transferase, glutathione peroxidase, catalase, and superoxide dismutase activities were significantly elevated. In conclusion, MNLE may have a potential role when combined with cisplatin in chemotherapy to alleviate cisplatin-induced damage and oxidative stress in liver.
authors.affiliation: Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia ; Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo 11795, Egypt.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Dkhil
authors.affiliation: Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Saleh
authors.initials: S
authors.lastname: Al-Quraishy
authors.affiliation: Biological Science Department, Faculty of Dentistry, Modern Science and Arts University (MSA), Giza 12111, Egypt.
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Aref
authors.affiliation: Biochemistry and Molecular Biology Department, Faculty of Pharmacy, Modern Science and Arts (MSA), Giza 12111, Egypt.
authors.forename: Mohamed S
authors.initials: MS
authors.lastname: Othman
authors.affiliation: Molecular Drug Evaluation Department, National Organization for Drug Control & Research (NODCAR), Giza 12553, Egypt.
authors.forename: Kamal M
authors.initials: KM
authors.lastname: El-Deib
authors.affiliation: Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo 11795, Egypt ; Department of Biochemistry and Molecular Biology, Asturias Institute of Biotechnology, University of Oviedo, Oviedo 33006, Spain.
authors.forename: Ahmed E
authors.initials: AE
authors.lastname: Abdel Moneim
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D031601:Azadirachta / Q000737:chemistry*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053453:Caspase 9 / Q000235:genetics / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D002945:Cisplatin / Q000009:adverse effects*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016328:NF-kappa B; D010936:Plant Extracts; D020011:Protective Agents; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D002374:Catalase; D013482:Superoxide Dismutase; D053148:Caspase 3; D053453:Caspase 9; D002945:Cisplatin