title: Functional imaging of glucose-evoked rat islet activities using transient intrinsic optical signals.
abstract: We demonstrate intrinsic optical signal (IOS) imaging of intact rat islet, which consists of many endocrine cells working together. A near-infrared digital microscope was employed for optical monitoring of islet activities evoked by glucose stimulation. Dynamic NIR images revealed transient IOS responses in the islet activated by low-dose (2.75mM) and high-dose (5.5mM) glucose stimuli. Comparative experiments and quantitative analysis indicated that both glucose metabolism and calcium/insulin dynamics might contribute to the observed IOS responses. Further investigation of the IOS imaging technology may provide a high resolution method forex vivofunctional examination of the islet, which is important for advanced study of diabetes associated islet dysfunctions and for improved quality control of donor islets for transplantation.
authors.affiliation: Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
authors.forename: Xin-Cheng
authors.initials: XC
authors.lastname: Yao
authors.affiliation: Department of Surgery, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
authors.forename: Wan-Xing
authors.initials: WX
authors.lastname: Cui
authors.affiliation: Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
authors.forename: Yi-Chao
authors.initials: YC
authors.lastname: Li
authors.affiliation: Department of Surgery, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
authors.forename: Rong-Wen
authors.initials: RW
authors.lastname: Lu
authors.affiliation: Department of Surgery, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
authors.forename: Anthony
authors.initials: A
authors.lastname: Thompson
authors.affiliation: Department of Psychology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
authors.forename: Franklin
authors.initials: F
authors.lastname: Amthor
authors.affiliation: Department of Physics, University of Alabama at Birmingham, Birmingham, AL 35294, USA ; The Comprehensive Diabetes Center, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
authors.forename: Xu-Jing
authors.initials: XJ
authors.lastname: Wang
keywords: functional imaging; glucose stimulation; intrinsic optical signal; rat islet