title: A secreted peptide acts on BIN2-mediated phosphorylation of ARFs to potentiate auxin response during lateral root development.
abstract: The phytohormone auxin is a key developmental signal in plants. So far, only auxin perception has been described to trigger the release of transcription factors termed Auxin Response Factors (ARFs) from their auxin/indole-3-acetic acid (AUX/IAA) repressor proteins. Here, we show that phosphorylation of ARF7 and ARF19 by BRASSINOSTEROID-insensitive2 (BIN2) can also potentiate auxin signalling output during lateral root organogenesis. BIN2-mediated phosphorylation of ARF7 and ARF19 suppresses their interaction with AUX/IAAs, and subsequently enhances the transcriptional activity to their target genes lateral organ boundaries-domain16 (LBD16) and LBD29. In this context, BIN2 is under the control of the Tracheary element differentiation inhibitory factor (TDIF)-TDIF receptor (TDR) module. TDIF-initiated TDR signalling directly acts on BIN2-mediated ARF phosphorylation, leading to the regulation of auxin signalling during lateral root development. In summary, this study delineates a TDIF-TDR-BIN2 signalling cascade that controls regulation of ARF and AUX/IAA interaction independent of auxin perception during lateral root development.
authors.affiliation: 1] Department of Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea [2].
authors.forename: Hyunwoo
authors.initials: H
authors.lastname: Cho
authors.affiliation: 1] Department of Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea [2].
authors.forename: Hojin
authors.initials: H
authors.lastname: Ryu
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang 790-784, Korea.
authors.forename: Sangchul
authors.initials: S
authors.lastname: Rho
authors.affiliation: 1] Division of Plant and Crop Sciences, School of Biosciences University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK [2] Centre for Plant Integrative Biology, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK.
authors.forename: Kristine
authors.initials: K
authors.lastname: Hill
authors.affiliation: Division of Plant and Crop Sciences, School of Biosciences University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Smith
authors.affiliation: 1] Integrative Plant Biology Division, Department of Plant Systems Biology, VIB, Technologiepark 927, Ghent 9052, Belgium [2] Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, Ghent 9052, Belgium.
authors.forename: Dominique
authors.initials: D
authors.lastname: Audenaert
authors.affiliation: Department of Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea.
authors.forename: Joonghyuk
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea.
authors.forename: Soeun
authors.initials: S
authors.lastname: Han
authors.affiliation: 1] Integrative Plant Biology Division, Department of Plant Systems Biology, VIB, Technologiepark 927, Ghent 9052, Belgium [2] Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, Ghent 9052, Belgium.
authors.forename: Tom
authors.initials: T
authors.lastname: Beeckman
authors.affiliation: 1] Division of Plant and Crop Sciences, School of Biosciences University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK [2] Centre for Plant Integrative Biology, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK.
authors.forename: Malcolm J
authors.initials: MJ
authors.lastname: Bennett
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang 790-784, Korea.
authors.forename: Daehee
authors.initials: D
authors.lastname: Hwang
authors.affiliation: 1] Division of Plant and Crop Sciences, School of Biosciences University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK [2] Integrative Plant Biology Division, Department of Plant Systems Biology, VIB, Technologiepark 927, Ghent 9052, Belgium [3] Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, Ghent 9052, Belgium.
authors.forename: Ive
authors.initials: I
authors.lastname: De Smet
authors.affiliation: Department of Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea.
authors.forename: Ildoo
authors.initials: I
authors.lastname: Hwang
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*; D018744:DNA, Plant / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008954:Models, Biological; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009842:Oligopeptides / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D011485:Protein Binding / Q000187:drug effects / Q000235:genetics; D011494:Protein Kinases / Q000378:metabolism*; D015398:Signal Transduction; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D029681:Arabidopsis Proteins; D018744:DNA, Plant; D007210:Indoleacetic Acids; D009842:Oligopeptides; D011494:Protein Kinases; D014157:Transcription Factors; C503426:ARF19 protein, Arabidopsis; C503425:ARF7 protein, Arabidopsis; C532424:tracheary element differentiation inhibitory factor, Arabidopsis; C448985:At4g18710 protein, Arabidopsis; C524131:PXY protein, Arabidopsis