title: The Amicon Pro system--a centrifugal device capable of performing all steps in the protein purification workflow.
abstract: raditional protein purification is a long process with many steps utilizing multiple devices, often resulting in protein degradation and loss. The Amicon Pro device streamlines the affinity purification process by providing a single adaptable centrifugation unit capable of performing all steps in the affinity purification process. The device combines affinity-based spin column purification with downstream sample concentration and buffer exchange, eliminating the need for multiple sample transfers, thereby minimizing protein loss. The results presented in this work indicate that purification of His-tagged protein using the Amicon Pro device is faster, easier, and provides better yields than other traditional methods (eg. spin-column and slurry method).
authors.affiliation: EMD Millipore, 17 Cherry Hill Drive, Danvers, MA 01923, USA. amedeo.cappione@merckgroup.com
authors.forename: Amedeo
authors.initials: A
authors.lastname: Cappione
authors.affiliation: EMD Millipore, 17 Cherry Hill Drive, Danvers, MA 01923, USA.
authors.forename: Masaharu
authors.initials: M
authors.lastname: Mabuchi
authors.affiliation: EMD Millipore, 17 Cherry Hill Drive, Danvers, MA 01923, USA.
authors.forename: Saosan
authors.initials: S
authors.lastname: Suhrawardy
authors.affiliation: EMD Millipore, 17 Cherry Hill Drive, Danvers, MA 01923, USA.
authors.forename: David
authors.initials: D
authors.lastname: Briggs
authors.affiliation: EMD Millipore, 17 Cherry Hill Drive, Danvers, MA 01923, USA.
authors.forename: Timothy
authors.initials: T
authors.lastname: Nadler
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D011506:Proteins