title: Bioassays guided isolation of compounds from Chaetomium globosum.
abstract: The aim of the present study was to evaluate different biological activities of the fungus Chaetomium globosum (family Chaetomiaceae). The evaluation was done through testing its antimicrobial, antioxidant and anticancer effects. C. globosum was isolated from the Cucumber soil (rhizosphere) and caused inhibition of the mycelial growth of Fusarium solani, Rhizoctonia solani and Sclerotium rolfsii in the biculture test. Petroleum ether and ethyl acetate extracts of the liquid culture of C. globosum showed potent in vitro antioxidant activity. C. globosum proved potent antibacterial activity against Bacillus subtilis, Escherichia coli and Pseudomonas fluorescens. It also recorded significant antifungal activity against Candida albicans, F. solani, Fusarium oxysporum, R. solani and Pythium ultimum. It exerted cytotoxic effect on human hepatocellular carcinoma cell line (HepG2). Unsaponifiable and saponifiable matters of the petroleum ether extract showed the presence of hydrocarbons, sterols and fatty acids. The ethyl acetate extract showed the presence of prenisatin, chrysophanol, chrysazin, chaetoviridin A and B. The isolated secondary metabolites proved significant antioxidant and antimicrobial activity on B. subtilis, E. coli and R. solani. In conclusion, this fungus showed different biological activities. Further studies must be done to apply its use in the agricultural and medicinal field.
authors.affiliation: Pharmacognosy Department, National Research Center, Dokki, Cairo, Egypt.
authors.forename: N E
authors.initials: NE
authors.lastname: Awad
authors.affiliation: Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
authors.forename: H A
authors.initials: HA
authors.lastname: Kassem
authors.affiliation: Therapeutic Chemistry Department, National Research Center, El-Tahrir Street, 12311 Dokki, Cairo, Egypt. Electronic address: manal_hamed@yahoo.com.
authors.forename: M A
authors.initials: MA
authors.lastname: Hamed
authors.affiliation: Plant pathology Department, National Research Center, Dokki, Cairo, Egypt.
authors.forename: M A A
authors.initials: MA
authors.lastname: El-Naggar
authors.affiliation: Pharmacognosy Department, National Research Center, Dokki, Cairo, Egypt.
authors.forename: A M M
authors.initials: AM
authors.lastname: El-Feky
mesh_terms: D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development; D001681:Biological Assay / Q000379:methods*; D002175:Candida / Q000187:drug effects / Q000254:growth & development; D002597:Chaetomium / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005670:Fusarium / Q000187:drug effects / Q000254:growth & development; D006801:Humans; D008826:Microbial Sensitivity Tests; D011551:Pseudomonas fluorescens / Q000187:drug effects / Q000254:growth & development; D011775:Pythium / Q000187:drug effects / Q000254:growth & development
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D000975:Antioxidants
keywords: Antibacterial; Antifungal; Antioxidant; Carcinome hépatocellulaire; Chaetomium; Effet antibactérien; Effet antifongique; Effet antioxydant; Hepatocellular carcinoma