title: Preparation of a sustained release drug delivery system for dexamethasone by a thermosensitive, in situ forming hydrogel for use in differentiation of dental pulp.
abstract: In situ forming delivery systems composed of block copolymers are attracting substantial attention due to their ease of use, biocompatibility, and biodegradability. In this study, the thermoresponsive triblock copolymer PLGA-PEG-PLGA was studied as a dexamethasone delivery system. Dexamethasone, a synthetic glucocorticoid, is used clinically to improve inflammation, pain, and the hyperemesis of chemotherapy, and it is applied experimentally as a differentiation factor in tissue engineering. PLGA-PEG-PLGA was synthesised under microwave irradiation for 5 min. The obtained copolymer was characterised to determine its structure and phase transition temperature. An in vitro release study was conducted for various copolymer structures and drug concentrations. The yield of the reaction and HNMR analysis confirmed the appropriateness of the microwave-assisted method for PLGA-PEG-PLGA synthesis. Phase transition temperature was affected by the drug molecule as well as by the copolymer concentration and structure. An in vitro release study demonstrated that release occurs mainly by diffusion and does not depend on the copolymer structure or dexamethasone concentration.
authors.affiliation: Targeted Drug Delivery Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Khodaverdi
authors.affiliation: Student Research Committee, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Kheirandish
authors.affiliation: Medical Nanotechnology Research Centre, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Farnaz Sadat
authors.initials: FS
authors.lastname: Mirzazadeh Tekie
authors.affiliation: Department of Medicinal Chemistry, School of Pharmacy, Tehran University of Medical Sciences, Mashhad, Iran.
authors.forename: Bibi Zahra
authors.initials: BZ
authors.lastname: Khashyarmanesh
authors.affiliation: Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Farzin
authors.initials: F
authors.lastname: Hadizadeh
authors.affiliation: Department of Medicinal Chemistry, School of Pharmacy, Tehran University of Medical Sciences, Mashhad, Iran.
authors.forename: Hamideh
authors.initials: H
authors.lastname: Moallemzadeh Haghighi