title: Time to detection of Mycobacterium tuberculosis using the MGIT 320 system correlates with colony counting in preclinical testing of new vaccines.
abstract: Clinical studies have suggested that the enumeration of mycobacteria by using automated liquid systems is a faster and simpler alternative to quantitative cultures. Here, we show that the time to detection of M. tuberculosis growth as measured with the MGIT 320 liquid culture system inversely correlates with CFU determinations from culture on solid media and that mycobacterial quantification using the MGIT system is faster and easier to perform than CFU plating.
authors.affiliation: Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Bethesda, Maryland, USA.
authors.forename: K
authors.initials: K
authors.lastname: Kolibab
authors.forename: A
authors.initials: A
authors.lastname: Yang
authors.forename: M
authors.initials: M
authors.lastname: Parra
authors.forename: S C
authors.initials: SC
authors.lastname: Derrick
authors.forename: S L
authors.initials: SL
authors.lastname: Morris
mesh_terms: D000818:Animals; D057205:Automation, Laboratory / Q000379:methods*; D001431:Bacteriological Techniques / Q000379:methods*; D015169:Colony Count, Microbial; D006801:Humans; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D013997:Time Factors; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology