title: [Comparison of two techniques for serological diagnosis of chlamydial infections: MIF and immunoblotting].
abstract: Sera from a population of 58 patients were used for comparison of two techniques for serodiagnosis of Chlamydia species: the MIF (micro-immunofluorescence) and immunoblotting. The MIF uses elementary bodies (EC) of Chlamydia while immunoblotting using recombinant proteins specific synthetic species of chlamydia. The objective of this study was to determine the sensitivity and specificity of serological techniques evaluated on a population control but also ill, by a comparative study between MIF which is the reference technique and immunoblotting (Chlamycheck). The technique Chlamycheck showed very good agreement with the MIF regarding Chlamydia trachomatis, but is a less good agreement with the MIF Chlamydia pneumoniae. The excellent specificity was observed with the technique Chlamycheck in the detection of anti-C. trachomatis, the useful sign of its use in the diagnosis of acute infections of Chlamydia trachomatis.
authors.affiliation: Laboratoire d'immunologie, CHU Ibn Rochd, Casablanca, Maroc.
authors.forename: Amal
authors.initials: A
authors.lastname: Saadouni
authors.affiliation: Laboratoire d'immunologie, CHU Ibn Rochd, Casablanca, Maroc.
authors.forename: Nezha
authors.initials: N
authors.lastname: Tbai
authors.affiliation: Laboratoire d'immunologie, CHU Ibn Rochd, Casablanca, Maroc.
authors.forename: Brahim
authors.initials: B
authors.lastname: Takourt
mesh_terms: D000328:Adult; D002690:Chlamydia Infections / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology; D002692:Chlamydia trachomatis / Q000276:immunology; D016993:Chlamydophila pneumoniae / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique / Q000379:methods; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D009018:Morocco / Q000453:epidemiology; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G
keywords: Chlamydia; MIF; immunoblot; serology