title: A sensitive HPLC-MS/MS method for the determination of dolutegravir in human plasma.
abstract: A sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) assay was developed and validated to facilitate the assessment of clinical pharmacokinetics of dolutegravir (DTG) in plasma samples. This work describes an assay system requiring only a 20μL aliquot of human plasma that is subjected to a simple acetonitrile protein precipitation containing a stably labeled isotope of DTG used as an internal standard. Chromatography was performed on an XBridge C18, 2.1mm×50mm, reversed phase analytical column, using a 60:40 acetonitrile/water mobile phase containing 0.1% formic acid. Detection of the analyte and internal standard was achieved by ESI positive ionization tandem mass spectrometry. The precursor/product transitions (m/z) monitored were 420.1/136.0 and 428.1/283.1 for DTG and DTG-IS, respectively. The dynamic range of this assay extends from 5 to 10,000ng/mL, with a mean coefficient of determination (r, mean±SD) of 0.9996±0.0003. The mean precision values for calibration standards ranged from 0.7 to 4.1%, while accuracy values were 98.3 to 102.0%. Validation results demonstrated high accuracy (≤6.5% deviation) and high precision (≤9.1% CV) for the quality control samples. This assay system provides an accurate, precise, and sensitive method for DTG quantitation and was successfully applied to clinical research samples as part of a phase I/II pediatric clinical trial.
authors.affiliation: Division of Clinical Pharmacology, University of Alabama at Birmingham School of Medicine, Birmingham, AL, United States.
authors.forename: Chantelle
authors.initials: C
authors.lastname: Bennetto-Hood
authors.affiliation: Bioanalytical Science and Toxicokinetics, PTS DMPK, GlaxoSmithKline, Research Triangle Park, NC, United States.
authors.forename: Glenn
authors.initials: G
authors.lastname: Tabolt
authors.affiliation: Biotransformation and Drug Disposition, PTS DMPK, GlaxoSmithKline, Research Triangle Park, NC, United States.
authors.forename: Paul
authors.initials: P
authors.lastname: Savina
authors.affiliation: Division of Clinical Pharmacology, University of Alabama at Birmingham School of Medicine, Birmingham, AL, United States. Electronic address: eacosta@uab.edu.
authors.forename: Edward P
authors.initials: EP
authors.lastname: Acosta
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019428:HIV Integrase Inhibitors / Q000097:blood*; D006575:Heterocyclic Compounds, 3-Ring / Q000097:blood*; D006801:Humans; D057230:Limit of Detection; D010078:Oxazines; D010879:Piperazines; D011728:Pyridones; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000099226:Dolutegravir
chemical_list: D019428:HIV Integrase Inhibitors; D006575:Heterocyclic Compounds, 3-Ring; D010078:Oxazines; D010879:Piperazines; D011728:Pyridones; D000099226:Dolutegravir
keywords: ARVs; AUC; AcN; CV; DMSO; DTG; Dolutegravir; EDTA; EFV; EVG; HIV; Human; INSTI; IPA; IS; Integrase inhibitor; LC–MS/MS; LLOQ; MRM; Mass spectrometry; MeOH; NRTI; PK; QC; RAL; UDP-glucuronosyl transferase 1A1; UGT1A1; ULQ; acetonitrile; antiretrovirals; area under the concentration–time curve; coefficient of variation; dimethyl sulfoxide; dolutegravir; efavirenz; elvitegravir; ethylenediamine tetraacetic acid; human immunodeficiency virus; integrase strand transfer inhibitor; internal standard; isopropanol; liquid chromatography tandem mass spectrometry; lower limit of quantitation; methanol; multi reaction monitoring; nucleoside reverse transcriptase inhibitor; pharmacokinetic; quality control; raltegravir; upper limit of quantitation