title: Comparison of agitake (Pleurotus eryngii var. ferulae) volatile components with characteristic odors extracted by hydrodistillation and solvent-assisted flavor evaporation.
abstract: The chemical composition of volatile oil from agitake (Pleurotus eryngii var. ferulae) was established for the first time using gas chromatography (GC) and GC-mass spectrometry. Sixty-seven and 24 components were extracted by hydrodistillation (HD) using diethyl ether (DE) and dichloromethane (DM), respectively; these components accounted for 80.3% and 91.8% of the total oil, respectively. Thirteen and 48 components of were extracted by the solvent-assisted flavor evaporation method (SAFE), using DE and DM, respectively, and identified; these components accounted for 83.5% and 82.0% of the total oil, respectively. Methylsuccinimide and 2,3,7-trimethyl-2-octene were the most characteristic components by SAFE using DM.Odor evaluation of the volatile oil from agitake was also carried out using GC-olfactometry (GC-O), aroma extraction dilution analysis (AEDA), and the odor activity value (OAV). Sixteen, 8, 5 and 9 aroma-active components were identified using HD (DE and DM) and SAFE (DE and DM), respectively. The main aroma-active components extracted using HD and SAFE were 1-octen-3-ol (mushroom-like) and phenylacetaldehyde (floral), respectively. This study proved that HD and SAFE can be used as complementary extraction techniques for the complete characterization of volatile oil from agitake.
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Usami
authors.forename: Toshirou
authors.initials: T
authors.lastname: Ono
authors.forename: Yusei
authors.initials: Y
authors.lastname: Kashima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakahashi
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Marumoto
authors.forename: Sota
authors.initials: S
authors.lastname: Nosaka
authors.forename: Shogo
authors.initials: S
authors.lastname: Watanabe
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Miyazawa
mesh_terms: D000079:Acetaldehyde / Q000031:analogs & derivatives / Q000032:analysis; D002849:Chromatography, Gas; D057168:Distillation / Q000379:methods; D004986:Ether; D008401:Gas Chromatography-Mass Spectrometry; D007201:Indicator Dilution Techniques; D008752:Methylene Chloride / Q000737:chemistry / Q000302:isolation & purification; D000442:Octanols / Q000032:analysis; D009812:Odorants / Q000032:analysis*; D009822:Oils, Volatile / Q000737:chemistry*; D064367:Olfactometry / Q000379:methods; D020076:Pleurotus / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D014835:Volatilization
chemical_list: D000442:Octanols; D009822:Oils, Volatile; D012997:Solvents; D004986:Ether; D008752:Methylene Chloride; D000079:Acetaldehyde; C013192:phenylacetaldehyde; C038844:1-octen-3-ol