title: Quantitative comparison of the autofluorescence of bacteria and polystyrene microspheres under violet wavelength excitation for verification of fluorescence-based bioaerosol detector results.
abstract: The autofluorescence intensity of bacteria and fungal spores was quantified by fluorescence microscopy in order to obtain the information for evaluating fluorescence-based bioaerosol detectors and was comparable to that of some types of polystyrene microspheres (PSMs). Although the intensity for microbes was distributed across a wide range over an order of magnitude in gray scale, it was in the intensity range of certain PSMs. Furthermore, some of those bacteria and PSMs were aerosolized in a test chamber and the fluorescence intensity was measured with a bioaerosol detector. Although there was a slight difference in the order of intensity from the results obtained by fluorescence microscopy, the fluorescence-based bioaerosol detector showed the intensity was in a comparable range.
authors.affiliation: Azbil Corporation.
authors.forename: Norio
authors.initials: N
authors.lastname: Hasegawa
mesh_terms: D000336:Aerosols; D015374:Biosensing Techniques / Q000379:methods; D055598:Chemical Phenomena; D005453:Fluorescence; D008027:Light; D008863:Microspheres; D011137:Polystyrenes / Q000737:chemistry* / Q000528:radiation effects; D013171:Spores, Bacterial / Q000737:chemistry* / Q000528:radiation effects; D013172:Spores, Fungal / Q000737:chemistry* / Q000528:radiation effects
chemical_list: D000336:Aerosols; D011137:Polystyrenes