title: [Study on sperm damage caused by trichloroethylene in male rats].
abstract: OBJECTIVE
  To study in vitro sperm damage caused by trichloroethylene in male rats.
  
  
  METHODS
  Sperms of Sprague-Dawley (SD) rats were collected 4 hours after being contaminated by trichloroethylene of 0, 2, 4, 6, 8, and 10 mmol/L in vitro. Giemsa staining was performed to observe the morphological changes of sperms, and flow cytometer was used to detect the changes in mitochondrial membrane potential.
  
  
  RESULTS
  The sperm motilities in 6, 8, and 10 mmol/L trichloroethylene groups decreased significantly compared with that in control group (P <0.01); the sperm aberration rates in 8 and 10 mmol/L trichloroethylene groups were significantly higher than that in control group (P<0.01). With the increase in exposure dose, the proportion of sperms with reduced mitochondrial membrane potential increased, and there were significant differences in sperm apoptosis rate between the 4, 6, 8, and 10 mmol/L trichloroethylene groups and control group (P<0.01).
  
  
  CONCLUSIONS
  In vitro exposure to trichloroethylene can reduce sperm motility and increase the aberration rate and apoptosis rate of sperms in male SD rats.
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of GuangDong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China.
authors.forename: De-sheng
authors.initials: DS
authors.lastname: Wu
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of GuangDong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China.
authors.forename: Lin-qing
authors.initials: LQ
authors.lastname: Yang
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of GuangDong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China.
authors.forename: Sui
authors.initials: S
authors.lastname: Huang
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of GuangDong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China.
authors.forename: Jian-jun
authors.initials: JJ
authors.lastname: Liu
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of GuangDong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China.
authors.forename: Xin-yun
authors.initials: XY
authors.lastname: Xu
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of GuangDong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China.
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Huang
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of GuangDong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China.
authors.forename: Chun-mei
authors.initials: CM
authors.lastname: Gong
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of GuangDong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China.
authors.forename: Gong-hua
authors.initials: GH
authors.lastname: Hu
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of GuangDong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China.
authors.forename: Qing-cheng
authors.initials: QC
authors.lastname: Liu
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of GuangDong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China.
authors.forename: Xi-fei
authors.initials: XF
authors.lastname: Yang
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of GuangDong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China.
authors.forename: Wen-xu
authors.initials: WX
authors.lastname: Hong
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of GuangDong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of GuangDong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China.
authors.forename: Xin-feng
authors.initials: XF
authors.lastname: Huang
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of GuangDong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China.
authors.forename: Jian-hui
authors.initials: JH
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of GuangDong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China. E-mail: zxzhuang2007@126.co.
authors.forename: Zhi-xiong
authors.initials: ZX
authors.lastname: Zhuang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects*; D014241:Trichloroethylene / Q000633:toxicity*
chemical_list: D014241:Trichloroethylene