title: High-resolution photoacoustic tomography of resting-state functional connectivity in the mouse brain.
abstract: The increasing use of mouse models for human brain disease studies presents an emerging need for a new functional imaging modality. Using optical excitation and acoustic detection, we developed a functional connectivity photoacoustic tomography system, which allows noninvasive imaging of resting-state functional connectivity in the mouse brain, with a large field of view and a high spatial resolution. Bilateral correlations were observed in eight functional regions, including the olfactory bulb, limbic, parietal, somatosensory, retrosplenial, visual, motor, and temporal regions, as well as in several subregions. The borders and locations of these regions agreed well with the Paxinos mouse brain atlas. By subjecting the mouse to alternating hyperoxic and hypoxic conditions, strong and weak functional connectivities were observed, respectively. In addition to connectivity images, vascular images were simultaneously acquired. These studies show that functional connectivity photoacoustic tomography is a promising, noninvasive technique for functional imaging of the mouse brain.
authors.affiliation: Optical Imaging Laboratory, Department of Biomedical Engineering and Department of Radiology, Washington University in St. Louis, St. Louis, MO 63130.
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Nasiriavanaki
authors.forename: Jun
authors.initials: J
authors.lastname: Xia
authors.forename: Hanlin
authors.initials: H
authors.lastname: Wan
authors.forename: Adam Quentin
authors.initials: AQ
authors.lastname: Bauer
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Culver
authors.forename: Lihong V
authors.initials: LV
authors.lastname: Wang
mesh_terms: D000465:Algorithms; D000818:Animals; D001921:Brain / Q000473:pathology; D001931:Brain Mapping / Q000379:methods*; D004195:Disease Models, Animal; D004566:Electrodes; D004867:Equipment Design; D006439:Hemodynamics; D006454:Hemoglobins / Q000378:metabolism; D018496:Hyperoxia; D000860:Hypoxia; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007834:Lasers; D008297:Male; D051379:Mice; D009434:Neural Pathways; D016011:Normal Distribution; D061088:Photoacoustic Techniques / Q000379:methods*
chemical_list: D006454:Hemoglobins
keywords: RSFC; fcPAT; hyperoxia; hypoxia; mouse brain functional imaging