title: Droplet array on local redox cycling-based electrochemical (LRC-EC) chip device.
abstract: We have previously reported a local redox cycling-based electrochemical (LRC-EC) system for the incorporation of many electrochemical sensors into a small chip device. In the present study, a new type of LRC-EC chip device was fabricated for the detection of a droplet array. To detect electrochemically redox compounds in droplets, Pt pseudo-reference/counter electrodes were incorporated into the individual sensors of the LRC-EC chip device. Cyclic voltammetry for the LRC-EC chip device with internal Pt pseudo-reference electrodes indicated well-defined voltammograms based on redox cycling for the individual sensor points. The device was successfully applied to the addressable detection of alkaline phosphatase (ALP) activity of HeLa cells in single droplets on the sensor points. Therefore, the LRC-EC chip device is considered to be a useful device for the bioanalysis of droplet systems.
authors.affiliation: Graduate School of Environmental Studies, Tohoku University, Japan. ino.kosuke@bioinfo.che.tohoku.ac.jp matsue@bioinfo.che.tohoku.ac.jp.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Ino
authors.forename: Takehito
authors.initials: T
authors.lastname: Goto
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kanno
authors.forename: Kumi Y
authors.initials: KY
authors.lastname: Inoue
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shiku
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Matsue
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D005296:Ferrous Compounds / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D057054:Molecular Imaging; D010084:Oxidation-Reduction
chemical_list: D005296:Ferrous Compounds; C071550:ferrocenemethanol; D000469:Alkaline Phosphatase