title: Ultrasensitive multi-analyte electrochemical immunoassay based on GNR-modified heated screen-printed carbon electrodes and PS@PDA-metal labels for rapid detection of MMP-9 and IL-6.
abstract: An ultrasensitive electrochemical immunoassay was developed for rapid detection of interleukin-6 (IL-6) and matrix metallopeptidase-9 (MMP-9); the method utilized PS@PDA-metal nanocomposites based on graphene nanoribbon (GNR)-modified heated screen-printed carbon electrode (HSPCE). Because of the good hydrophilicity and low toxicity, GNRs were used to immobilize antibodies (Ab) and amplify the electrochemical signal. PS@PDA-metal was used to label antibodies and generate a strong electrochemical signal in acetic buffer. A sandwich strategy was adopted to achieve simultaneous detection of MMP-9 and IL-6 based on HSPCE without cross-talk between adjacent electrodes in the range of 10(-5) to 10(3) ng mL(-1) with detection limits of 5 fg mL(-1) and 0.1 pg mL(-1) (S/N=3), respectively. The proposed method showed wide detection range, low detection limit, acceptable stability and good reproducibility. Satisfactory results were also obtained in the practical samples, thus showing this is a promising technique for simultaneous clinical detection of biocomponent proteins.
authors.affiliation: School of Chemical Engineering, Anhui University of Science and Technology, Huainan 232001, China; State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Shi
authors.affiliation: School of Chemical Engineering, Anhui University of Science and Technology, Huainan 232001, China; State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: He
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Jiang
authors.affiliation: Petrochemical Research Chair, Department of Chemistry, College of Science, King Saud University, Riyadh 11451, P.O. Box 2455, Kingdom of Saudi Arabia.
authors.forename: E S
authors.initials: ES
authors.lastname: Abdel-Halim
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. Electronic address: jjzhu@nju.edu.cn.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhu
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation*; D002244:Carbon / Q000737:chemistry*; D045424:Complex Mixtures / Q000032:analysis / Q000276:immunology; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D015850:Interleukin-6 / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D020780:Matrix Metalloproteinase 9 / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D054802:Molecular Imprinting / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling
chemical_list: D000906:Antibodies; D045424:Complex Mixtures; D015850:Interleukin-6; D011137:Polystyrenes; D002244:Carbon; D020780:Matrix Metalloproteinase 9
keywords: Electrochemical immunoassay; Graphene nanoribbon; Heated screen-printed carbon electrode; Multi-analyte; Polymer@metal nanocomposites