title: Rat adipose tissue-derived stem cells attenuate peritoneal injuries in rat zymosan-induced peritonitis accompanied by complement activation.
abstract: OBJECTIVE
  In patients receiving peritoneal dialysis, fungal or yeast peritonitis has a poor prognosis. In rat peritoneum with mechanical scraping, severe peritonitis can be induced by zymosan, a component of yeast (Zy/scraping peritonitis). Administration of rat adipose tissue-derived stromal cells (ASCs) potentially can improve several tissue injuries. The present study investigated whether rat ASCs could improve peritoneal inflammation in Zy/scraping peritonitis.
  
  
  METHODS
  Rat ASCs were injected intraperitoneally on a daily basis in rats with Zy/scraping peritonitis.
  
  
  RESULTS
  Peritoneal inflammation accompanied by accumulation of inflammatory cells and complement deposition was suppressed by day 5 after injection of rat ASCs. The peritoneal mesothelial layer in Zy/scraping peritonitis with rat ASC treatment was restored compared with the peritoneal mesothelial layer without rat ASC treatment. Injected rat ASCs co-existed with mesothelial cells in the sub-peritoneal layer. In vitro assays showed increased cellular proliferation of rat mesothelial cells combined with rat ASCs by co-culture assays, confirming that fluid factors from rat ASCs might play some role in facilitating the recovery of rat mesothelial cells. Hepatocyte growth factor was released from rat ASCs, and administration of recombinant hepatocyte growth factor increased rat mesothelial cell proliferation.
  
  
  CONCLUSIONS
  Because the peritoneal mesothelium shows strong expression of membrane complement regulators such as Crry, CD55 and CD59, restoration of the mesothelial cell layer by rat ASCs might prevent deposition of complement activation products and ameliorate peritoneal injuries. This study suggests the therapeutic possibilities of intraperitoneal rat ASC injection to suppress peritoneal inflammation by restoring the mesothelial layer and decreasing complement activation in fungal or yeast peritonitis.
authors.affiliation: Division of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Hangsoo
authors.initials: H
authors.lastname: Kim
authors.affiliation: Division of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan; Renal Replacement Therapy, Nagoya University Graduate School of Medicine, Nagoya, Japan. Electronic address: mmizu@med.nagoya-u.ac.jp.
authors.forename: Masashi
authors.initials: M
authors.lastname: Mizuno
authors.affiliation: Division of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Furuhashi
authors.affiliation: Division of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Katsuno
authors.affiliation: Division of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Takenori
authors.initials: T
authors.lastname: Ozaki
authors.affiliation: Division of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Yasuda
authors.affiliation: Division of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Naotake
authors.initials: N
authors.lastname: Tsuboi
authors.affiliation: Division of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Waichi
authors.initials: W
authors.lastname: Sato
authors.affiliation: Division of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan; Renal Replacement Therapy, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: Division of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Matsuo
authors.affiliation: Division of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan; Renal Replacement Therapy, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Ito
authors.affiliation: Division of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Shoichi
authors.initials: S
authors.lastname: Maruyama
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D003167:Complement Activation; D004195:Disease Models, Animal; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D006801:Humans; D007274:Injections, Intraperitoneal; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010538:Peritonitis / Q000139:chemically induced / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015003:Yeasts / Q000378:metabolism*; D015054:Zymosan / Q000008:administration & dosage / Q000378:metabolism
chemical_list: D017228:Hepatocyte Growth Factor; D015054:Zymosan
keywords: adipose-derived stromal cells; complement; membrane complement regulators; peritoneal dialysis; peritonitis