title: Simultaneous imaging of protonated and deprotonated carbonylcyanide p-trifluoromethoxyphenylhydrazone in live cells by Raman microscopy.
abstract: We report the simultaneous imaging of protonated and deprotonated forms of carbonylcyanide p-trifluoromethoxy-phenylhydrazone (FCCP) molecules in live cells by Raman microscopy. Nitriles, structure-sensitive Raman tags, are used to detect the two distinct molecular structures, demonstrating the potential of Raman microscopy for structure-based imaging of bioactive small molecules.
authors.affiliation: Sodeoka Live Cell Chemistry Project, ERATO, Japan Sciences and Technology Agency, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan. sodeoka@riken.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamakoshi
authors.forename: Almar F
authors.initials: AF
authors.lastname: Palonpon
authors.forename: Kosuke
authors.initials: K
authors.lastname: Dodo
authors.forename: Jun
authors.initials: J
authors.lastname: Ando
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kawata
authors.forename: Katsumasa
authors.initials: K
authors.lastname: Fujita
authors.forename: Mikiko
authors.initials: M
authors.lastname: Sodeoka
mesh_terms: D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000737:chemistry*; D002467:Cell Nucleus / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008853:Microscopy / Q000379:methods; D011522:Protons; D013059:Spectrum Analysis, Raman
chemical_list: D008055:Lipids; D011522:Protons; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone