title: Rapid pretreatment and detection of trace aflatoxin B1 in traditional soybean sauce.
abstract: Soybean sauce, a traditional fermented food in China, has different levels of aflatoxin B1 pollution. Two kinds of direct and indirect immunomagnetic bead methods for the pretreatment of aflatoxin B1 were evaluated in this work. A method was established to detect aflatoxin B1 in soybean sauce using an immunomagnetic bead system for pretreatment and ELISA for quantification. The pretreatment method of immunomagnetic beads performed better compared with the conventional extraction and immunoaffinity column method. ELISA exhibited a good linear relationship at an aflatoxin B1 concentration of 0.05-0.3μg/kg (r(2)=0.9842). The average recoveries across spike levels varied from 0.5 to 7μg/kg were 83.6-104% with a relative standard deviation between 4.2% and 11.7%. With the advantages of rapid detection, easy operation, simple equipment, sensitivity, accuracy, and high recovery; this method can be well applied in the trace determination of aflatoxin B1 in soybean sauce samples.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address: talktolaiwh@163.com.
authors.forename: WeiHua
authors.initials: W
authors.lastname: Lai
authors.affiliation: Department of Animal Sciences and Industry, Kansas State University, 66506, Manhattan, KS, USA.
authors.forename: Jasdeep
authors.initials: J
authors.lastname: Saini
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Shan
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: DaoFeng
authors.initials: D
authors.lastname: Liu
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis* / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D018189:Immunomagnetic Separation / Q000379:methods*; D045730:Soy Foods / Q000032:analysis*
chemical_list: D016604:Aflatoxin B1
keywords: Aflatoxin B(1); ELISA; Immunomagnetic beads; Soybean sauce; Trace