title: Spatial gene's (Tbata) implication in neurite outgrowth and dendrite patterning in hippocampal neurons.
abstract: The unique architecture of neurons requires the establishment and maintenance of polarity, which relies in part on microtubule-based kinesin motor transport to deliver essential cargo into axons and dendrites. In developing neurons, kinesin trafficking is essential for delivering organelles and molecules that are crucial for elongation and guidance of the growing axonal and dendritic termini. In mature neurons, kinesin cargo delivery is essential for neuron dynamic physiological functions which are critical in brain development. In this work, we followed Spatial (Tbata) gene expression during primary hippocampal neuron development and showed that it is highly expressed during dendrite formation. Spatial protein exhibits a somatodendritic distribution and we show that the kinesin motor Kif17, among other dendrite specific kinesins, is crucial for Spatial localization to dendrites of hippocampal neurons. Furthermore, Spatial down regulation in primary hippocampal cells revealed a role for Spatial in maintaining neurons' polarity by ensuring proper neurite outgrowth. This polarity is specified by intrinsic and extracellular signals that allow neurons to determine axon and dendrite fate during development. Neurotrophic factors, such as the Nerve Growth Factor (NGF), are candidate extracellular polarity-regulating cues which are proposed to accelerate neuronal polarization by enhancing dendrite growth. Here, we show that NGF treatment increases Spatial expression in hippocampal neurons. Altogether, these data suggest that Spatial, in response to NGF and through its transport by Kif17, is crucial for neuronal polarization and can be a key regulator of neurite outgrowth.
authors.affiliation: Aix-Marseille Université, Inserm UMR 1090, TAGC, Campus de Luminy Case 908, 13288 Marseille Cedex 9, France.
authors.forename: Miriam
authors.initials: M
authors.lastname: Yammine
authors.affiliation: Instituto de Biología Molecular de Barcelona, CSIC, Parc Cientific de Barcelona, C/Baldiri i Reixac, Barcelona, Spain.
authors.forename: Murielle
authors.initials: M
authors.lastname: Saade
authors.affiliation: Aix-Marseille Université, CNRS UMR 7288, IBDM, Campus de Luminy Case 908, 13288 Marseille Cedex 9, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Chauvet
authors.affiliation: Aix-Marseille Université, Inserm UMR 1090, TAGC, Campus de Luminy Case 908, 13288 Marseille Cedex 9, France. Electronic address: catherine.nguyen@inserm.fr.
authors.forename: Catherine
authors.initials: C
authors.lastname: Nguyen
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D006801:Humans; D016547:Kinesins / Q000378:metabolism; D051379:Mice; D020932:Nerve Growth Factor / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects / Q000378:metabolism*; D055495:Neurogenesis; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport
chemical_list: C409642:KIF17 protein, mouse; D009687:Nuclear Proteins; C419984:spatial protein, mouse; D020932:Nerve Growth Factor; D016547:Kinesins
keywords: Kinesin Kif17; Neurite outgrowth; Neuronal polarization; Spatial gene