title: Interactome of two diverse RNA granules links mRNA localization to translational repression in neurons.
abstract: Transport of RNAs to dendrites occurs in neuronal RNA granules, which allows local synthesis of specific proteins at active synapses on demand, thereby contributing to learning and memory. To gain insight into the machinery controlling dendritic mRNA localization and translation, we established a stringent protocol to biochemically purify RNA granules from rat brain. Here, we identified a specific set of interactors for two RNA-binding proteins that are known components of neuronal RNA granules, Barentsz and Staufen2. First, neuronal RNA granules are much more heterogeneous than previously anticipated, sharing only a third of the identified proteins. Second, dendritically localized mRNAs, e.g., Arc and CaMKIIα, associate selectively with distinct RNA granules. Third, our work identifies a series of factors with known roles in RNA localization, translational control, and RNA quality control that are likely to keep localized transcripts in a translationally repressed state, often in distinct types of RNPs.
authors.affiliation: Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria.
authors.forename: Renate
authors.initials: R
authors.lastname: Fritzsche
authors.affiliation: Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria; Max-Planck-Institute for Developmental Biology, 72076 Tübingen, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Karra
authors.affiliation: CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, 1090 Vienna, Austria.
authors.forename: Keiryn L
authors.initials: KL
authors.lastname: Bennett
authors.affiliation: Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria; Department for Anatomy & Cell Biology, Ludwig-Maximilians-University, 80336 Munich, Germany.
authors.forename: Foong Yee
authors.initials: FY
authors.lastname: Ang
authors.affiliation: Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria.
authors.forename: Jacki E
authors.initials: JE
authors.lastname: Heraud-Farlow
authors.affiliation: Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria.
authors.forename: Marco
authors.initials: M
authors.lastname: Tolino
authors.affiliation: Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Doyle
authors.affiliation: Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria; Department for Anatomy & Cell Biology, Ludwig-Maximilians-University, 80336 Munich, Germany.
authors.forename: Karl E
authors.initials: KE
authors.lastname: Bauer
authors.affiliation: Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria; Max-Planck-Institute for Developmental Biology, 72076 Tübingen, Germany; Department for Anatomy & Cell Biology, Ludwig-Maximilians-University, 80336 Munich, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Thomas
authors.affiliation: CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, 1090 Vienna, Austria.
authors.forename: Melanie
authors.initials: M
authors.lastname: Planyavsky
authors.affiliation: Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria; Max-Planck-Institute for Developmental Biology, 72076 Tübingen, Germany.
authors.forename: Eric
authors.initials: E
authors.lastname: Arn
authors.affiliation: Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria.
authors.forename: Anetta
authors.initials: A
authors.lastname: Bakosova
authors.affiliation: Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria; Max-Planck-Institute for Developmental Biology, 72076 Tübingen, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Jungwirth
authors.affiliation: Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Hörmann
authors.affiliation: Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria.
authors.forename: Zsofia
authors.initials: Z
authors.lastname: Palfi
authors.affiliation: Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria.
authors.forename: Julia
authors.initials: J
authors.lastname: Sandholzer
authors.affiliation: Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria.
authors.forename: Martina
authors.initials: M
authors.lastname: Schwarz
authors.affiliation: Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria; Max-Planck-Institute for Developmental Biology, 72076 Tübingen, Germany.
authors.forename: Paolo
authors.initials: P
authors.lastname: Macchi
authors.affiliation: CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, 1090 Vienna, Austria.
authors.forename: Jacques
authors.initials: J
authors.lastname: Colinge
authors.affiliation: CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, 1090 Vienna, Austria.
authors.forename: Giulio
authors.initials: G
authors.lastname: Superti-Furga
authors.affiliation: Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, Austria; Max-Planck-Institute for Developmental Biology, 72076 Tübingen, Germany; Department for Anatomy & Cell Biology, Ludwig-Maximilians-University, 80336 Munich, Germany. Electronic address: michael.kiebler@med.uni-muenchen.de.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Kiebler
mesh_terms: D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000235:genetics / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D003712:Dendrites / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis; D034443:RNA Transport; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism; D051381:Rats; D012261:Ribonucleoproteins / Q000378:metabolism
chemical_list: C476130:Casc3 protein, rat; D003598:Cytoskeletal Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C492188:Stau2 protein, rat; C092069:activity regulated cytoskeletal-associated protein; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2