title: The mechanism of sevoflurane preconditioning-induced protections against small intestinal ischemia reperfusion injury is independent of mast cell in rats.
abstract: The study aimed to investigate whether sevoflurane preconditioning can protect against small intestinal ischemia reperfusion (IIR) injury and to explore whether mast cell (MC) is involved in the protections provided by sevoflurane preconditioning. Sprague-Dawley rats exposed to sevoflurane or treated with MC stabilizer cromolyn sodium (CS) were subjected to 75-minute superior mesenteric artery occlusion followed by 2-hour reperfusion in the presence or absence of MC degranulator compound 48/80 (CP). Small intestinal ischemia reperfusion resulted in severe intestinal injury as demonstrated by significant elevations in intestinal injury scores and p47(phox) and gp91(phox), ICAM-1 protein expressions and malondialdehyde and IL-6 contents, and MPO activities as well as significant reductions in SOD activities, accompanied with concomitant increases in mast cell degranulation evidenced by significant increases in MC counts, tryptase expression, and β-hexosaminidase concentrations, and those alterations were further upregulated in the presence of CP. Sevoflurane preconditioning dramatically attenuated the previous IIR-induced alterations except MC counts, tryptase, and β-hexosaminidase which were significantly reduced by CS treatment. Furthermore, CP exacerbated IIR injury was abrogated by CS but not by sevoflurane preconditioning. The data collectively indicate that sevoflurane preconditioning confers protections against IIR injury, and MC is not involved in the protective process.
authors.affiliation: Department of Anesthesiology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China ; Department of Anesthesiology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Gan
authors.affiliation: Department of Anesthesiology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Guangjie
authors.initials: G
authors.lastname: Su
authors.affiliation: Department of Anesthesiology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China ; Department of Anesthesiology, The First People's Hospital of Foshan, Foshan 528000, China.
authors.forename: Weicheng
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Anesthesiology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Pinjie
authors.initials: P
authors.lastname: Huang
authors.affiliation: Department of Anesthesiology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Gangjian
authors.initials: G
authors.lastname: Luo
authors.affiliation: Department of Anesthesiology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Ziqing
authors.initials: Z
authors.lastname: Hei
mesh_terms: D018685:Anesthetics, Inhalation / Q000494:pharmacology; D000818:Animals; D004205:Cromolyn Sodium / Q000494:pharmacology; D005260:Female; D007124:Immunoenzyme Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000473:pathology; D007421:Intestine, Small / Q000187:drug effects / Q000473:pathology*; D008315:Malondialdehyde / Q000378:metabolism; D008407:Mast Cells / Q000187:drug effects / Q000473:pathology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D017538:Mesenteric Artery, Superior / Q000187:drug effects / Q000473:pathology; D008738:Methyl Ethers / Q000494:pharmacology*; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology* / Q000517:prevention & control*; D000077149:Sevoflurane; D053802:Tryptases / Q000378:metabolism; D001619:beta-N-Acetylhexosaminidases / Q000097:blood
chemical_list: D018685:Anesthetics, Inhalation; D015850:Interleukin-6; D008562:Membrane Glycoproteins; D008738:Methyl Ethers; D018799:Intercellular Adhesion Molecule-1; D000077149:Sevoflurane; D008315:Malondialdehyde; D009195:Peroxidase; C500593:Cybb protein, rat; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1; D001619:beta-N-Acetylhexosaminidases; D053802:Tryptases; D004205:Cromolyn Sodium