title: A controlled release of antibiotics from calcium phosphate-coated poly(lactic-co-glycolic acid) particles and their in vitro efficacy against Staphylococcus aureus biofilm.
abstract: Ceramic-polymer hybrid particles, intended for osteomyelitis treatment, were fabricated by preparing poly(lactic-co-glycolic acid) particles through an emulsion solvent evaporation technique, followed by calcium phosphate (CaP) coating via a surface adsorption-nucleation method. The presence of CaP coating on the surface of the particles was confirmed by scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray photoelectron spectroscopy. Subsequently, two antibiotics for treating bone infection, nafcillin (hydrophilic) and levofloxacin (amphiphilic), were loaded into these hybrid particles and their in vitro drug release studies were investigated. The CaP coating was shown to reduce burst release, while providing sustained release of the antibiotics for up to 4 weeks. In vitro bacterial study against Staphylococcus aureus demonstrated the capability of these antibiotic-loaded hybrid particles to inhibit biofilm formation as well as deteriorate established biofilm, making this hybrid system a potential candidate for further investigation for osteomyelitis treatment.
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore.
authors.forename: Kelsen
authors.initials: K
authors.lastname: Bastari
authors.forename: Mohamed
authors.initials: M
authors.lastname: Arshath
authors.forename: Zhi Hui Melissa
authors.initials: ZH
authors.lastname: Ng
authors.forename: Jia Hua
authors.initials: JH
authors.lastname: Chia
authors.forename: Zhi Xian Daniel
authors.initials: ZX
authors.lastname: Yow
authors.forename: Barindra
authors.initials: B
authors.lastname: Sana
authors.forename: Meng Fong Cherine
authors.initials: MF
authors.lastname: Tan
authors.forename: Sierin
authors.initials: S
authors.lastname: Lim
authors.forename: Say Chye Joachim
authors.initials: SC
authors.lastname: Loo
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D002130:Calcium Phosphates / Q000737:chemistry*; D002470:Cell Survival; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000737:chemistry*; D004058:Diffusion; D053769:Nanocapsules / Q000008:administration & dosage* / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011100:Polyglycolic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D002130:Calcium Phosphates; D003692:Delayed-Action Preparations; D053769:Nanocapsules; C523618:calcium phosphate polylactic-co-glycolic acid composite; D011100:Polyglycolic Acid