title: Molecularly imprinted solid-phase extraction monolithic capillary column for selective extraction and sensitive determination of safranine T in wolfberry.
abstract: A method was developed to sensitively determine safranine T in wolfberry by molecularly imprinted solid-phase extraction (MISPE) coupled with high-performance liquid chromatography and laser-induced fluorescence detection (HPLC-LIF). The MISPE capillary monolithic column was prepared by water-bath in situ polymerization, using safranine T, methacrylic acid (MAA), and ethylene dimethacrylate (EDMA) as template, functional monomer, and cross-linker, respectively. The properties of the homemade MISPE capillary monolithic column, including capacity and specificity, were investigated under optimized conditions and the morphologies of inner polymers were characterized by scanning electron microscopy (SEM). The mean recoveries of safranine T in wolfberry ranged from 91.2 % to 92.9 % and the intraday and interday relative standard deviation (RSD) values all ranged from 3.4 % to 4.2 %. Good linearity was obtained over 0.001-1.0 μg mL(-1) (r = 0.9999) with a detection limit (S/N = 3) of 0.4 ng g(-1). Under the selected conditions, enrichment factors of over 90-fold were obtained and the extraction on the monolithic column effectively cleaned up the wolfberry matrix. The results demonstrated that the proposed MISPE-HPLC-LIF method could be applied to sensitively determine safranine T in wolfberry.
authors.affiliation: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China.
authors.forename: Zihao
authors.initials: Z
authors.lastname: Su
authors.forename: Haiyun
authors.initials: H
authors.lastname: Zhai
authors.forename: Zuanguang
authors.initials: Z
authors.lastname: Chen
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhou
authors.forename: Jiangmei
authors.initials: J
authors.lastname: Li
authors.forename: Zhenping
authors.initials: Z
authors.lastname: Liu
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D057230:Limit of Detection; D032305:Lycium / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry; D054802:Molecular Imprinting; D010619:Phenazines / Q000302:isolation & purification*; D052616:Solid Phase Extraction
chemical_list: D008689:Methacrylates; D010619:Phenazines; C008384:methacrylic acid; C004919:ethylene dimethacrylate; C009195:safranine T