title: [Molecular genetic analysis of the isoniazid-resistant strains of M. tuberculosis circulating over the Saratov region].
abstract: 257 patients with active lung tuberculosis in the Saratov region were tested for distribution and mutation spectrum of the genes katG, inhA, ahpC encoding Isoniazid-resistance. The tests were implemented using the biological microchips with pharmacological kits TV-Biochip MDR (Russia). The rate of the Isoniazid-resistant M. tuberculosis strains incidence was found to be 55.7%. The Isoniazid-resistant strains demonstrated high rate (26.9%) of combined mutations in 2 and 3 genes simultaneously, domination in the katG ser315 -> Thrl (91.1%), thereby inducing high resistance to Isoniazid. The sensitivity of the TV-Biochip MDR test-system was 88.2%; the specificity, 91.3%. The results ofthis work show that the incidence of the Isoniazid-resistant strains should be limited using additional measures.
authors.forename: T Iu
authors.initials: TIu
authors.lastname: Salina
authors.forename: T I
authors.initials: TI
authors.lastname: Morozova
mesh_terms: D000995:Antitubercular Agents; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002374:Catalase / Q000235:genetics* / Q000378:metabolism; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005260:Female; D006801:Humans; D007538:Isoniazid; D008297:Male; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D010802:Phylogeny; D012426:Russia; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism
chemical_list: D000995:Antitubercular Agents; D001426:Bacterial Proteins; D002374:Catalase; C480353:katG protein, Mycobacterium tuberculosis; D007538:Isoniazid