title: Expression pattern of Class B gene PAP3 in flower development of pepper.
abstract: Class B gene APETALA3 (AP3) plays a key role in the development of petals and stamens. Here, we investigated the expression pattern of PAP3 gene (genbank accession number: HM104635) in the buds of cytoplasmic male sterility line 121A and its near-isogenic restorer line 121C at four developmental stages and analyzed the possible association between Class B genes and cytoplasmic male sterility of pepper. Semi-quantitative PCR and quantitative real-time RT-PCR (qRT-PCR) as well as RNA in situ hybridization showed increased expression of PAP3 at late phase of anther development and its higher expression in restorer line compared with sterility line indicating PAP3's role at late developmental stage of anther and suppressed expression in sterility line. RNA in situ hybridization showed Class B gene features: high abundance in stamen and petal; lower expression in pistil; no expression in sepal. Results of transient expression in onion epidermal cells also showed PAP3 localized in the nucleus, which is consistent with the expression pattern of transcription factors of MADS-box gene family.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Chen
authors.initials: C
authors.lastname: Liu
authors.forename: Fengjiao
authors.initials: F
authors.lastname: Da
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.affiliation: Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing 100193, China. SHL1606@cau.edu.cn.
authors.forename: Huolin
authors.initials: H
authors.lastname: Shen
mesh_terms: D002212:Capsicum / Q000378:metabolism*; D003001:Cloning, Molecular; D035264:Flowers / Q000378:metabolism; D017403:In Situ Hybridization; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D012313:RNA