title: BMP-9 induces rabbit adipose-derived stem cells to differentiation into osteoblasts via BMP signaling pathway.
abstract: OBJECTIVE
  To explore the role of BMP-9 in osteoblast differentiation from adipose-derived stem cells (ADSCs).
  
  
  METHODS
  Rabbit ADSCs were isolated from subcutaneous tissues of the abdomen and inguinal fat pads and then purified and expanded in vitro. AdBMP9, SB203580 (P38 MAPK inhibitors), and PD98059 (ERK1/2 inhibitor) were used for osteoblastic differentiation.
  
  
  RESULTS
  The results show that bone morphogenetic proteins (BMPs) could affect the differential direction of ADSCs. Polymerase chain reaction assays reveal the important role of BMP signaling pathway in osteoblastic differentiation of ADSCs, and the members included Smad 1, Smad 4, Smad 5, Smad 8, P38, ERK1/2, Runx 2, collagen type I and osteopontin.
  
  
  CONCLUSIONS
  This study provides some theoretical basis and experimental evidence for the application of ADSCs into treatment of bone injury.
authors.affiliation: Department of Spine Surgery, Third Hospital of HeBei Medical University, Shijiazhuang, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Chai
authors.affiliation: Department of Spine Surgery, Third Hospital of HeBei Medical University, Shijiazhuang, China.
authors.forename: Fajing
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Spine Surgery, Third Hospital of HeBei Medical University, Shijiazhuang, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Spine Surgery, Third Hospital of HeBei Medical University, Shijiazhuang, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Spine Surgery, Third Hospital of HeBei Medical University, Shijiazhuang, China.
authors.forename: Wen Yuan
authors.initials: WY
authors.lastname: Ding
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000818:Animals; D052004:Bone Morphogenetic Protein Receptors / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D055427:Growth Differentiation Factor 2 / Q000008:administration & dosage / Q000378:metabolism*; D007093:Imidazoles / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D011725:Pyridines / Q000494:pharmacology; D011817:Rabbits; D015398:Signal Transduction / Q000187:drug effects; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D055427:Growth Differentiation Factor 2; D007093:Imidazoles; D011725:Pyridines; D048051:p38 Mitogen-Activated Protein Kinases; D052004:Bone Morphogenetic Protein Receptors; C093642:SB 203580