title: The effects of polymorphisms on human gene targeting.
abstract: DNA mismatches that occur between vector homology arms and chromosomal target sequences reduce gene targeting frequencies in several species; however, this has not been reported in human cells. Here we demonstrate that even a single mismatched base pair can significantly decrease human gene targeting frequencies. In addition, we show that homology arm polymorphisms can be used to direct allele-specific targeting or to improve unfavorable vector designs that introduce deletions.
authors.affiliation: Department of Medicine, University of Washington, Seattle, WA, 98195, USA and Department of Biochemistry, University of Washington, Seattle, WA, 98195, USA.
authors.forename: David R
authors.initials: DR
authors.lastname: Deyle
authors.forename: Li B
authors.initials: LB
authors.lastname: Li
authors.forename: Gaoying
authors.initials: G
authors.lastname: Ren
authors.forename: David W
authors.initials: DW
authors.lastname: Russell
mesh_terms: D020137:Base Pair Mismatch; D045744:Cell Line, Tumor; D002875:Chromosomes / Q000737:chemistry; D018390:Gene Targeting; D056426:Genetic Loci; D005822:Genetic Vectors / Q000737:chemistry; D006801:Humans; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide