title: Rapid and sensitive liquid chromatography coupled with electrospray ionization tandem mass spectrometry method for the analysis of paclitaxel, docetaxel, vinblastine, and vinorelbine in human plasma.
abstract: BACKGROUND
  A rapid and sensitive analytical method using liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-MS/MS) was developed for the determination of paclitaxel, docetaxel, vinblastine, and vinorelbine in human plasma.
  
  
  METHODS
  A simple liquid-liquid extraction procedure was applied using only 100-μL plasma. Chromatographic separation of these anticancer drugs was achieved with an isocratic mobile phase consisting of acetonitrile/aqueous buffer (10 mmol/L ammonium acetate and 0.1% formic acid in 70:30, vol/vol) at a flow rate of 0.25 mL/min in a short time (4.5 minutes).
  
  
  RESULTS
  The calibration curves for paclitaxel, docetaxel, vinblastine, and vinorelbine in spiked human plasma ranged from 25 to 2500, 10 to 1000, 10 to 1000, and 10 to 1000 ng/mL, respectively. The squares of the linear correlation coefficients were all more than 0.99. The intraday and interday relative standard deviations across 3 validation runs over the entire concentration range were less than 9.2%.
  
  
  CONCLUSIONS
  The established method should be helpful for the pharmacokinetic monitoring of paclitaxel, docetaxel, vinblastine, and vinorelbine in the human plasma of non-small cell lung cancer patients.
authors.affiliation: Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Shouhong
authors.initials: S
authors.lastname: Gao
authors.forename: Jingya
authors.initials: J
authors.lastname: Zhou
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Haijun
authors.initials: H
authors.lastname: Miao
authors.forename: Yunlei
authors.initials: Y
authors.lastname: Yun
authors.forename: Jin
authors.initials: J
authors.lastname: Feng
authors.forename: Xia
authors.initials: X
authors.lastname: Tao
authors.forename: Wansheng
authors.initials: W
authors.lastname: Chen
mesh_terms: D000970:Antineoplastic Agents / Q000097:blood*; D002138:Calibration; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy*; D002851:Chromatography, High Pressure Liquid; D000077143:Docetaxel; D004305:Dose-Response Relationship, Drug; D016903:Drug Monitoring / Q000379:methods*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy*; D008297:Male; D017239:Paclitaxel / Q000097:blood; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D043823:Taxoids / Q000097:blood; D014747:Vinblastine / Q000031:analogs & derivatives / Q000097:blood; D000077235:Vinorelbine
chemical_list: D000970:Antineoplastic Agents; D043823:Taxoids; D000077143:Docetaxel; D014747:Vinblastine; D017239:Paclitaxel; D000077235:Vinorelbine