title: High level of resistance to aztreonam and ticarcillin in Pseudomonas aeruginosa isolated from soil of different crops in Brazil.
abstract: Pseudomonas aeruginosa can be found in water, soil, plants and, human and animal fecal samples. It is an important nosocomial pathogenic agent characterized by an intrinsic resistance to multiple antimicrobial agents and the ability to develop high-level (acquired) multidrug resistance through some mechanisms, among them, by the acquisition of plasmids and integrons, which are mobile genetic elements. In this study, 40 isolates from Brazilian soil were analyzed for antibiotic resistance, presence of integrons and plasmidial profile. The results demonstrated that the vast majority of the isolates have shown resistance for aztreonam (92.5%, n=37) and ticarcillin (85%, n=34), four isolates presented plasmids and eight isolates possess the class 1 integron. These results demonstrated that environmental isolates of P. aeruginosa possess surprising antibiotic resistance profile to aztreonam and ticarcillin, two antimicrobial agents for clinical treatment of cystic fibrosis patients and other infections occurred by P. aeruginosa.
authors.affiliation: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Ribeirão Preto, Brazil.
authors.forename: André
authors.initials: A
authors.lastname: Pitondo-Silva
authors.affiliation: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Ribeirão Preto, Brazil.
authors.forename: Vinicius Vicente
authors.initials: VV
authors.lastname: Martins
authors.affiliation: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Ribeirão Preto, Brazil.
authors.forename: Ana Flavia Tonelli
authors.initials: AF
authors.lastname: Fernandes
authors.affiliation: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Ribeirão Preto, Brazil. Electronic address: elianags@usp.br.
authors.forename: Eliana Guedes
authors.initials: EG
authors.lastname: Stehling
mesh_terms: D000900:Anti-Bacterial Agents / Q000633:toxicity*; D001398:Aztreonam / Q000633:toxicity*; D001938:Brazil; D018556:Crops, Agricultural / Q000382:microbiology*; D024881:Drug Resistance, Bacterial / Q000502:physiology*; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D013982:Ticarcillin / Q000633:toxicity*
chemical_list: D000900:Anti-Bacterial Agents; D012987:Soil; D013982:Ticarcillin; D001398:Aztreonam
keywords: Antibiotic resistance; Plasmids; Pseudomonas aeruginosa; Soil