title: The Wnt/planar cell polarity signaling pathway contributes to the integrity of tight junctions in brain endothelial cells.
abstract: Wnt morphogens released by neural precursor cells were recently reported to control blood-brain barrier (BBB) formation during development. Indeed, in mouse brain endothelial cells, activation of the Wnt/β-catenin signaling pathway, also known as the canonical Wnt pathway, was shown to stabilize endothelial tight junctions (TJs) through transcriptional regulation of the expression of TJ proteins. Because Wnt proteins activate several distinct β-catenin-dependent and independent signaling pathways, this study was designed to assess whether the noncanonical Wnt/Par/aPKC planar cell polarity (PCP) pathway might also control TJ integrity in brain endothelial cells. First we established, in the hCMEC/D3 human brain endothelial cell line, that the Par/aPKC PCP complex colocalizes with TJs and controls apicobasal polarization. Second, using an siRNA approach, we showed that the Par/aPKC PCP complex regulates TJ stability and reassembling after osmotic shock. Finally, we provided evidence that Wnt5a signals in hCMEC/D3 cells through activation of the Par/aPKC PCP complex, independently of the Wnt canonical β-catenin-dependent pathway and significantly contributes to TJ integrity and endothelial apicobasal polarity. In conclusion, this study suggests that the Wnt/Par/aPKC PCP pathway, in addition to the Wnt/β-catenin canonical pathway, is a key regulator of the BBB.
authors.affiliation: 1] INSERM U1016, Institut Cochin, Paris, France [2] CNRS, UMR 8104, Paris, France [3] Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Artus
authors.affiliation: 1] INSERM U1016, Institut Cochin, Paris, France [2] CNRS, UMR 8104, Paris, France [3] Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Glacial
authors.affiliation: 1] INSERM U1016, Institut Cochin, Paris, France [2] CNRS, UMR 8104, Paris, France [3] Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: Kayathiri
authors.initials: K
authors.lastname: Ganeshamoorthy
authors.affiliation: Institute of Neurology (Edinger-Institute), Johann Wolfgang Goethe-University Frankfurt Medical School, Frankfurt, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Ziegler
authors.affiliation: 1] INSERM U1016, Institut Cochin, Paris, France [2] CNRS, UMR 8104, Paris, France [3] Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: Maeva
authors.initials: M
authors.lastname: Godet
authors.affiliation: INSERM U1016, Institut Cochin, Paris, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Guilbert
authors.affiliation: Institute of Neurology (Edinger-Institute), Johann Wolfgang Goethe-University Frankfurt Medical School, Frankfurt, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Liebner
authors.affiliation: 1] INSERM U1016, Institut Cochin, Paris, France [2] CNRS, UMR 8104, Paris, France [3] Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: Pierre-Olivier
authors.initials: PO
authors.lastname: Couraud
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D001812:Blood-Brain Barrier / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D018929:Cell Culture Techniques; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D016764:Cell Polarity; D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010539:Permeability; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D019108:Tight Junctions / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism*; D060449:Wnt Signaling Pathway / Q000235:genetics / Q000502:physiology*; D000071818:Wnt-5a Protein
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; D008565:Membrane Proteins; C509689:PARD3 protein, human; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; C115015:PKC-3 protein; D011493:Protein Kinase C