title: Middle East Respiratory Syndrome coronavirus (MERS-CoV) serology in major livestock species in an affected region in Jordan, June to September 2013.
abstract: Between June and September 2013, sera from 11 dromedary camels, 150 goats, 126 sheep and 91 cows were collected in Jordan, where the first human Middle-East respiratory syndrome (MERS) cluster appeared in 2012. All sera were tested for MERS-coronavirus (MERS-CoV) specific antibodies by protein microarray with confirmation by virus neutralisation. Neutralising antibodies were found in all camel sera while sera from goats and cattle tested negative. Although six sheep sera reacted with MERS-CoV antigen, neutralising antibodies were not detected.
authors.affiliation: These authors contributed equally to this work.
authors.forename: C B
authors.initials: CB
authors.lastname: Reusken
authors.forename: M
authors.initials: M
authors.lastname: Ababneh
authors.forename: V S
authors.initials: VS
authors.lastname: Raj
authors.forename: B
authors.initials: B
authors.lastname: Meyer
authors.forename: A
authors.initials: A
authors.lastname: Eljarah
authors.forename: S
authors.initials: S
authors.lastname: Abutarbush
authors.forename: G J
authors.initials: GJ
authors.lastname: Godeke
authors.forename: T M
authors.initials: TM
authors.lastname: Bestebroer
authors.forename: I
authors.initials: I
authors.lastname: Zutt
authors.forename: M A
authors.initials: MA
authors.lastname: Muller
authors.forename: B J
authors.initials: BJ
authors.lastname: Bosch
authors.forename: P J
authors.initials: PJ
authors.lastname: Rottier
authors.forename: A D
authors.initials: AD
authors.lastname: Osterhaus
authors.forename: C
authors.initials: C
authors.lastname: Drosten
authors.forename: B L
authors.initials: BL
authors.lastname: Haagmans
authors.forename: M P
authors.initials: MP
authors.lastname: Koopmans
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D002162:Camelus / Q000097:blood*; D002417:Cattle; D017934:Coronavirus / Q000276:immunology* / Q000302:isolation & purification; D018352:Coronavirus Infections / Q000097:blood; D005260:Female; D006041:Goats / Q000097:blood; D006801:Humans; D007597:Jordan; D058751:Livestock; D046228:Microarray Analysis; D008877:Middle East; D009500:Neutralization Tests; D060888:Real-Time Polymerase Chain Reaction; D012141:Respiratory Tract Infections / Q000209:etiology; D012756:Sheep / Q000097:blood; D013577:Syndrome
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral