title: Optimization of marine waste based-growth media for microbial lipase production using mixture design methodology.
abstract: Lipase production by Staphylococcus xylosus and Rhizopus oryzae was investigated using a culture medium based on a mixture of synthetic medium and supernatants generated from tuna by-products and Ulva rigida biomass. The proportion of the three medium components was optimized using the simplex-centroid mixture design method (SCMD). Results indicated that the experimental data were in good agreement with predicted values, indicating that SCMD was a reliable method for determining the optimum mixture proportion of the growth medium. Maximal lipase activities of 12.5 and 23.5 IU/mL were obtained with a 50:50 (v:v) mixture of synthetic medium and tuna by-product supernatant for Staphylococcus xylosus and Rhizopus oryzae, respectively. The predicted responses from these mixture proportions were also validated experimentally.
authors.affiliation: Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS, Université de Sfax, Sfax, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Sellami
authors.affiliation: Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS, Université de Sfax, Sfax, Tunisia.
authors.forename: Samiha
authors.initials: S
authors.lastname: Kedachi
authors.affiliation: Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS, Université de Sfax, Sfax, Tunisia.
authors.forename: Fakher
authors.initials: F
authors.lastname: Frikha
authors.affiliation: Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS, Université de Sfax, Sfax, Tunisia.
authors.forename: Nabil
authors.initials: N
authors.lastname: Miled
authors.affiliation: Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS, Université de Sfax, Sfax, Tunisia.
authors.forename: Faouzi
authors.initials: F
authors.lastname: Ben Rebah
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D019149:Bioreactors; D003470:Culture Media; D005525:Food-Processing Industry; D005656:Fungal Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D007220:Industrial Waste; D008049:Lipase / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D015233:Models, Statistical; D012037:Refuse Disposal / Q000379:methods*; D015203:Reproducibility of Results; D012107:Research Design; D012233:Rhizopus / Q000737:chemistry / Q000378:metabolism; D013210:Staphylococcus / Q000737:chemistry / Q000378:metabolism; D014413:Tuna; D044445:Ulva
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D005656:Fungal Proteins; D007220:Industrial Waste; D008049:Lipase