title: Prevention of peritoneal adhesions using polymeric rheological blends.
abstract: The effectiveness of rheological blends of high molecular weight hyaluronic acid (HA) and low molecular weight hydroxypropyl methylcellulose (HPMC) in the prevention of peritoneal adhesions post-surgery is demonstrated. The physical mixture of the two carbohydrates increased the dwell time in the peritoneum while significantly improving the injectability of the polymer compared with HA alone. HA-HPMC treatment decreased the total adhesion area by ∼ 70% relative to a saline control or no treatment in a repeated cecal injury model in the rabbit. No significant cytotoxicity and minimal inflammation were associated with the blend. Furthermore, no chemical or physical processing was required prior to their use beyond simple mixing.
authors.affiliation: Department of Chemical Engineering, McMaster University, 1280 Main St. W., Hamilton, Ontario L8S 4L7, Canada.
authors.forename: Todd
authors.initials: T
authors.lastname: Hoare
authors.affiliation: Department of Industrial and Physical Pharmacy, Purdue University, 575 Stadium Mall Dr., West Lafayette, IN 47907, USA.
authors.forename: Yoon
authors.initials: Y
authors.lastname: Yeo
authors.affiliation: Department of Materials Science, Academic Centre for Dentistry Amsterdam, Gustav Mahjerlaan 3004, 10B1 LA Amsterdam, The Netherlands.
authors.forename: Evangelia
authors.initials: E
authors.lastname: Bellas
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, MA 02139, USA.
authors.forename: Joost P
authors.initials: JP
authors.lastname: Bruggeman
authors.affiliation: Laboratory for Biomaterials and Drug Delivery, Department of Anesthesiology, Division of Critical Care Medicine, Children's Hospital Boston, Harvard Medical School, 300 Longwood Ave., Boston, MA 02115, USA. Electronic address: Daniel.Kohane@childrens.harvard.edu.
authors.forename: Daniel S
authors.initials: DS
authors.lastname: Kohane
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004195:Disease Models, Animal; D006801:Humans; D006820:Hyaluronic Acid / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D065347:Hypromellose Derivatives / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D010537:Peritoneum / Q000187:drug effects / Q000473:pathology*; D011817:Rabbits; D012212:Rheology; D000267:Tissue Adhesions / Q000517:prevention & control*
chemical_list: D065347:Hypromellose Derivatives; D006820:Hyaluronic Acid
keywords: Hyaluronic acid; Hydroxypropyl methylcellulose; Peritoneal adhesions; Rheological blends