title: Application of three-dimensional imaging to the intestinal crypt organoids and biopsied intestinal tissues.
abstract: Two-dimensional (2D) histopathology is the standard analytical method for intestinal biopsied tissues; however, the role of 3-dimensional (3D) imaging system in the analysis of the intestinal tissues is unclear. The 3D structure of the crypt organoids from the intestinal stem cell culture and intestinal tissues from the donors and recipients after intestinal transplantation was observed using a 3D imaging system and compared with 2D histopathology and immunohistochemistry. The crypt organoids and intestinal tissues showed well-defined 3D structures. The 3D images of the intestinal tissues with acute rejection revealed absence of villi and few crypts, which were consistent with the histopathological features. In the intestinal transplant for megacystis microcolon intestinal hypoperistalsis syndrome, the donor's intestinal tissues had well-developed nerve networks and interstitial cells of Cajal (ICCs) in the muscle layer, while the recipient's intestinal tissues had distorted nerve network and the ICCs were few and sparsely distributed, relative to those of the donor. The 3D images showed a clear spatial relationship between the microstructures of the small bowel and the features of graft rejection. In conclusion, integration of the 3D imaging and 2D histopathology provided a global view of the intestinal tissues from the transplant patients.
authors.affiliation: Department of Surgery, Far Eastern Memorial Hospital, Pan-Chiao, New Taipei 220, Taiwan ; Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li, Taoyuan 320, Taiwan.
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Surgery, Far Eastern Memorial Hospital, Pan-Chiao, New Taipei 220, Taiwan ; Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li, Taoyuan 320, Taiwan.
authors.forename: Ya-Hui
authors.initials: YH
authors.lastname: Tsai
authors.affiliation: Department of Chemical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan.
authors.forename: Yuan-An
authors.initials: YA
authors.lastname: Liu
authors.affiliation: Department of Surgery, Far Eastern Memorial Hospital, Pan-Chiao, New Taipei 220, Taiwan.
authors.forename: Shih-Hua
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Surgery, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei 100, Taiwan.
authors.forename: Sheng-Hong
authors.initials: SH
authors.lastname: Tseng
authors.affiliation: Department of Chemical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan ; Department of Medical Science, Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan.
authors.forename: Shiue-Cheng
authors.initials: SC
authors.lastname: Tang
mesh_terms: D000015:Abnormalities, Multiple / Q000473:pathology / Q000601:surgery; D000818:Animals; D001706:Biopsy; D003106:Colon / Q000002:abnormalities / Q000473:pathology / Q000601:surgery; D006084:Graft Rejection / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional / Q000379:methods; D056885:Interstitial Cells of Cajal / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D007418:Intestinal Pseudo-Obstruction / Q000473:pathology / Q000601:surgery; D007421:Intestine, Small / Q000166:cytology / Q000378:metabolism / Q000473:pathology / Q000637:transplantation; D007422:Intestines / Q000166:cytology* / Q000473:pathology* / Q000637:transplantation; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D009197:Myenteric Plexus / Q000378:metabolism / Q000473:pathology; D061848:Optical Imaging / Q000379:methods; D009940:Organoids; D011419:Propidium / Q000737:chemistry; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D013194:Staining and Labeling; D001743:Urinary Bladder / Q000002:abnormalities / Q000473:pathology / Q000601:surgery
chemical_list: D011419:Propidium; D019009:Proto-Oncogene Proteins c-kit