title: Sublytic C5b-9 induces IL-6 and TGF-β1 production by glomerular mesangial cells in rat Thy-1 nephritis through p300-mediated C/EBPβ acetylation.
abstract: CCAAT/enhancer-binding protein (C/EBPβ)-enhanced IL-6 and TGF-β1 promoter activity and p300-mediated C/EBPβ acetylation were involved in up-regulation of IL-6 and TGF-β1 expression in GMCs attacked by sublytic C5b-9. In detail, the elements of C/EBPβ binding to rat IL-6 and TGF-β1 promoter and 3 acetylated sites of rat C/EBPβ protein were first revealed. Furthermore, silencing the p300 or C/EBPβ gene in rat kidney significantly reduced the production of IL-6 and TGF-β1 and renal lesions in Thy-1N rats. Together, these data indicate that the mechanism of IL-6 and TGF-β1 production in renal tissue of Thy-1N rats is associated with sublytic C5b-9 up-regulated p300 and p300-mediated C/EBPβ acetylation as well as C/EBPβ-activated IL-6 and TGF-β1 genes.
authors.affiliation: 1Department of Microbiology and Immunology, Nanjing Medical University, Hanzhong Rd. 140, Nanjing, Jiangsu 210029, China. wangyw1508@njmu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Kai
authors.initials: K
authors.lastname: Shan
authors.forename: Lulu
authors.initials: L
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Qiu
authors.forename: Yanlai
authors.initials: Y
authors.lastname: Lu
authors.forename: Dan
authors.initials: D
authors.lastname: Zhao
authors.forename: Ganqian
authors.initials: G
authors.lastname: Zhu
authors.forename: Fengxia
authors.initials: F
authors.lastname: He
authors.forename: Yingwei
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000107:Acetylation; D000818:Animals; D001483:Base Sequence; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000378:metabolism*; D002478:Cells, Cultured; D015938:Complement Membrane Attack Complex / Q000502:physiology*; D017931:DNA Primers; D005920:Glomerular Mesangium / Q000166:cytology / Q000378:metabolism*; D020381:Interleukin-17 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis*; D008297:Male; D009393:Nephritis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis*; D050880:p300-CBP Transcription Factors / Q000378:metabolism*
chemical_list: D022782:CCAAT-Enhancer-Binding Protein-beta; D015938:Complement Membrane Attack Complex; D017931:DNA Primers; D020381:Interleukin-17; D015850:Interleukin-6; D053773:Transforming Growth Factor beta1; D050880:p300-CBP Transcription Factors
keywords: post-translational modification; renal inflammation; renal pathology; transcription factor