title: Effects of DL-homocysteine thiolactone on cardiac contractility, coronary flow, and oxidative stress markers in the isolated rat heart: the role of different gasotransmitters.
abstract: Considering the adverse effects of DL-homocysteine thiolactone hydrochloride (DL-Hcy TLHC) on vascular function and the possible role of oxidative stress in these mechanisms, the aim of this study was to assess the influence of DL-Hcy TLHC alone and in combination with specific inhibitors of important gasotransmitters, such as L-NAME, DL-PAG, and PPR IX, on cardiac contractility, coronary flow, and oxidative stress markers in an isolated rat heart. The hearts were retrogradely perfused according to the Langendorff technique at a 70 cm H2O and administered 10  μM DL-Hcy TLHC alone or in combination with 30  μM L-NAME, 10  μM DL-PAG, or 10  μM PPR IX. The following parameters were measured: dp/dt max, dp/dt min, SLVP, DLVP, MBP, HR, and CF. Oxidative stress markers were measured spectrophotometrically in coronary effluent through TBARS, NO2, O2(-), and H2O2 concentrations. The administration of DL-Hcy TLHC alone decreased dp/dt max, SLVP, and CF but did not change any oxidative stress parameters. DL-Hcy TLHC with L-NAME decreased CF, O2(-), H2O2, and TBARS. The administration of DL-Hcy TLHC with DL-PAG significantly increased dp/dt max but decreased DLVP, CF, and TBARS. Administration of DL-Hcy TLHC with PPR IX caused a decrease in dp/dt max, SLVP, HR, CF, and TBARS.
authors.affiliation: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Zivkovic
authors.affiliation: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Jakovljevic
authors.affiliation: Institute of Normal and Pathological Physiology and Centre of Excellence for Examination of Regulatory Role of Nitric Oxide in Civilization Diseases, Slovak Academy of Sciences, Bratislava, Slovakia.
authors.forename: Olga
authors.initials: O
authors.lastname: Pechanova
authors.affiliation: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Srejovic
authors.affiliation: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
authors.forename: Jovana
authors.initials: J
authors.lastname: Joksimovic
authors.affiliation: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
authors.forename: Dragica
authors.initials: D
authors.lastname: Selakovic
authors.affiliation: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
authors.forename: Nevena
authors.initials: N
authors.lastname: Barudzic
authors.affiliation: Institute of Medical Physiology "Richard Burian", Faculty of Medicine University of Belgrade, Belgrade, Serbia.
authors.forename: Dragan M
authors.initials: DM
authors.lastname: Djuric
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D003326:Coronary Circulation / Q000187:drug effects*; D064426:Gasotransmitters / Q000378:metabolism*; D006321:Heart / Q000187:drug effects; D006710:Homocysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009200:Myocardial Contraction / Q000187:drug effects*; D009206:Myocardium / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D015415:Biomarkers; D064426:Gasotransmitters; D006710:Homocysteine; D006861:Hydrogen Peroxide; C007957:homocysteine thiolactone