title: USP28 is a potential prognostic marker for bladder cancer.
abstract: This study was conducted to analyze the expression of the ubiquitin-specific protease Usp28 and assess its clinical significance in human bladder cancer. mRNA and protein expression levels of Usp28 were determined by real-time polymerase chain reaction (PCR) and Western blot in 24 paired bladder cancers and the adjacent non-cancerous tissues. In addition, the expression of Usp28 protein in 186 bladder cancers was also determined by immunohistochemistry. The relationship between expression of Usp28 and clinico-pathologic features and prognosis was finally evaluated. Usp28 was expressed at a higher level in bladder cancers compared to adjacent non-cancerous tissues at both the mRNA and protein levels in 24 paired samples (all P < 0.01). In immunohistochemical examination, 78 (41.9%) of 186 cases displayed low Usp28 expression in cancerous tissues, whereas 108 (58.1%) cases displayed high Usp28 expression. In the universal analysis, Usp28 correlated strongly with histopathological grade, clinical stage, tumor number and recurrence rate (P = 0.0001, 0.0001, 0.0001 and 0.0051, respectively), but did not correlate with gender or age (P = 0.5588 and 0.6574). After multiple analysis of the above factors and consideration of confounding factors, tumor number, histological grade, clinical stage, and recurrence were related to Usp28 expression (P = 0.001, 0.001, 0.001 and 0.001, respectively). Finally, Usp28 expression was indentified as a independent predictors of survival (P = 0.001). Usp28 protein expression is potentially valuable in prognostic evaluation of bladder cancer.
authors.affiliation: Department of Urinary Surgery, The First Affiliated Hospital of Harbin Medical University, 23 You Zheng Street, Harbin, 150001, Heilongjiang, China.
authors.forename: Guiying
authors.initials: G
authors.lastname: Guo
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Xu
authors.forename: Mancheng
authors.initials: M
authors.lastname: Gong
authors.forename: Yan
authors.initials: Y
authors.lastname: Cao
authors.forename: Ruihua
authors.initials: R
authors.lastname: An
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D005260:Female; D016259:Genes, myc; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D016016:Proportional Hazards Models; D043222:Ubiquitin Thiolesterase / Q000032:analysis* / Q000235:genetics; D001749:Urinary Bladder Neoplasms / Q000737:chemistry / Q000401:mortality* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C442902:USP28 protein, human; D043222:Ubiquitin Thiolesterase