title: [Suppression of mitochondrial oxidative phosphorylation on in vitro maturation, fertilization and developmental competence of oocytes].
abstract: OBJECTIVE
  To investigate the roles of mitochondrial oxidative phosphorylation (OXPHOS) capacity in oocyte maturation, fertilization and embryo development.
  
  
  METHODS
  Carbonyl cyanide p- (tri-fluromethoxy) phenyl-hydrazone (FCCP), a metabolic inhibitor of mitochondria, was introduced into culture medium. The integrity of spindle and chromosome alignment, reactive oxygen species (ROS) levels, and rates of maturation, germinal vesicle breakdown, fertilization and blastulation were assessed in vitro.
  
  
  RESULTS
  Significant decreases were detected in the percentages of oocytes with nuclear maturation, normal spindle formation and chromosome alignment, ROS levels and capable for blastocyst formation between oocytes treated with FCCP and non-treated (control group), 55.8%, 37.9%, 0.67 and 57.9% (FCCP 10 nmol/L group), 47.3%,34.7%, 0.59 and 41.8% (FCCP 100 nmol/L group) versus 62.9%, 61.9%,0.94 and 68.3% (control group) respectively, P<0.05. However, No significant differences were found in the rates of GVBD and fertilization in oocytes from the FCCP treated and the control.
  
  
  CONCLUSIONS
  Inhibition of mitochondrial metabolic capacity resulted in decreased the percentages of oocytes with nuclear maturation, normal spindle formation and chromosome alignment, ROS levels and capable for blastocyst formation. But the treatment of FCCP did not affect the rate of fertilization.
authors.affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, Zhejiang Wenzhou 325000, China.
authors.forename: Hong-shan
authors.initials: HS
authors.lastname: Ge
authors.forename: Xiao-he
authors.initials: XH
authors.lastname: Li
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Chen
authors.forename: Hai-tao
authors.initials: HT
authors.lastname: Xi
authors.forename: Jian-ying
authors.initials: JY
authors.lastname: Huang
authors.forename: Chun-fang
authors.initials: CF
authors.lastname: Zhu
authors.forename: Jie-qiang
authors.initials: JQ
authors.lastname: Lv
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D004272:DNA, Mitochondrial / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008928:Mitochondria / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism / Q000502:physiology*; D009866:Oogenesis / Q000187:drug effects / Q000502:physiology*; D010085:Oxidative Phosphorylation; D017382:Reactive Oxygen Species
chemical_list: D004272:DNA, Mitochondrial; D017382:Reactive Oxygen Species; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D000255:Adenosine Triphosphate