title: Use of in vivo-induced antigen technology to identify in vivo-expressed genes of Campylobacter jejuni during human infection.
abstract: Campylobacter jejuni is a prevalent foodborne pathogen worldwide. Human infection by C. jejuni primarily arises from contaminated poultry meats. Genes expressed in vivo may play an important role in the pathogenicity of C. jejuni. We applied an immunoscreening method, in vivo-induced antigen technology (IVIAT), to identify in vivo-induced genes during human infection by C. jejuni. An inducible expression library of genomic proteins was constructed from sequenced C. jejuni NCTC 11168 and was then screened using adsorbed, pooled human sera obtained from clinical patients. We successfully identified 24 unique genes expressed in vivo. These genes were implicated in metabolism, molecular biosynthesis, genetic information processing, transport, and other processes. We selected six genes with different functions to compare their expression levels in vivo and in vitro using real-time RT-PCR. The results showed that the selected six genes were significantly upregulated in vivo but not in vitro. In short, these identified in vivo-induced genes may contribute to human infection of C. jejuni, some of which may be meaningful vaccine candidate antigens or diagnosis serologic markers for campylobacteriosis. IVIAT may present a significant and efficient method for understanding the pathogenicity mechanism of Campylobacter and for finding targets for its prevention and control.
authors.affiliation: Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Jiangsu 225009, PR China.
authors.forename: Yuanqing
authors.initials: Y
authors.lastname: Hu
authors.forename: Jinlin
authors.initials: J
authors.lastname: Huang
authors.forename: Qiuchun
authors.initials: Q
authors.lastname: Li
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Shang
authors.forename: Fangzhe
authors.initials: F
authors.lastname: Ren
authors.forename: Yang
authors.initials: Y
authors.lastname: Jiao
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Liu
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Pan
authors.forename: Xin-An
authors.initials: XA
authors.lastname: Jiao
mesh_terms: D002169:Campylobacter Infections / Q000382:microbiology*; D016123:Campylobacter jejuni / Q000235:genetics* / Q000302:isolation & purification; D005517:Foodborne Diseases / Q000382:microbiology; D020869:Gene Expression Profiling / Q000379:methods*; D005798:Genes, Bacterial; D006801:Humans; D007118:Immunoassay; D060888:Real-Time Polymerase Chain Reaction