title: A new fluorescent turn-on chemodosimeter for mercury ions in solution and its application in cells and organisms.
abstract: Using the Hg(2+)-induced desulfurization reaction of thiosemicarbazide derivative, we designed and synthesized a novel "turn on" coumarin-based fluorescent probe L with a simple structure for detecting mercury ion (II). Spectroscopy revealed that the probe responds selectively to mercury ions over other metal ions with marked fluorescence enhancement. Detection of Hg(2+) was effective at pH 7.0-9.5, with high selectivity and significant effect in HeLa cells, human umbilical vein endothelial cells and Escherichia coli, but no cytotoxicity. This probe could be an ideal and practical Hg(2+) probe with important biological significance.
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, PR China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Zhang
authors.affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, PR China. Electronic address: miaojy@sdu.edu.cn.
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Miao
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China. Electronic address: bxzhao@sdu.edu.cn.
authors.forename: Bao-Xiang
authors.initials: BX
authors.lastname: Zhao
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D003374:Coumarins / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D006834:Hydrazines / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000737:chemistry; D008628:Mercury / Q000032:analysis*; D008670:Metals / Q000737:chemistry; D018613:Microscopy, Confocal; D012996:Solutions / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; D006834:Hydrazines; D007477:Ions; D008670:Metals; C000590559:N-(2-(7-(diethylamino)-2-oxo-2H-chromene-3-carbonyl)hydrazinecarbonothioyl)-4-methoxybenzamide; D012996:Solutions; C030123:coumarin; D008628:Mercury
keywords: Coumarin; E. coli; Fluorescence probe; Mammalian cells; Mercury (II) ion