title: [Optimization of conditions for human erythropoiesis in human-mouse xenotransplant model].
abstract: This study was purposed to investigate the conditions for improving human-mouse xenograft and the erythroid differentiation of human hematopoietic stem cells (HSC) in the xenotransplant model. The engraftments of different mouse strains (NOD/SCID or NOD/SCID/IL2rγ(null)), schemes of irradiation (single-time or 2-times radiation; Co(60)γ-ray or X-ray) and strategies of CB CD34(+) cells ex vivo culture time and lentivirus infection were compared. The results showed that at 4 weeks after transplantation, the ratio of hCD45 positive cells in bone marrow of NOD/SCID/IL2rγ(null) mice increased to (51.4 ± 13.9)%, and erythroid precursor could be detected. All of the mice receiving X-ray irradiation for 2 times (a dose of 1 Gy, then the second of 1.5 Gy, with an interval of 15 min) survived. Fresh isolated CB CD34(+) cells were cultured and infected with lentivirus for 72 h and then transplanted into receptor mouse. After 4 weeks, higher engraftment [hCD45 (51.4 ± 13.9)%] and better erythroid development [hCD71(+) GPA(+) (5.98 ± 3.46)%] were observed. It is concluded that NOD/SCID/IL2rγ(null) mice receiving X-ray irradiation for 2 times and were injected with fresh isolated CB CD34(+) cells cultured and infected with lentivirus ex vivo within 72 h show a better xenograft and erythroid development.
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Zheng
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China; Department of Pathophysiology, Chinese PLA Genaral Hospital, Beijing 100853, China.
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: Xu
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Rong-Hua
authors.initials: RH
authors.lastname: Yin
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Yi-Qun
authors.initials: YQ
authors.lastname: Zhan
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Yang
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China. E-mail: fmmli@163.com.
authors.forename: Zhang-Yan
authors.initials: ZY
authors.lastname: Li
mesh_terms: D000818:Animals; D018952:Antigens, CD34; D001854:Bone Marrow Cells / Q000166:cytology; D002478:Cells, Cultured; D004912:Erythrocytes / Q000166:cytology*; D004920:Erythropoiesis; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D014183:Transplantation, Heterologous
chemical_list: D018952:Antigens, CD34