title: Choroid plexus implants rescue Alzheimer's disease-like pathologies by modulating amyloid-β degradation.
abstract: The choroid plexuses (CP) release numerous biologically active enzymes and neurotrophic factors, and contain a subpopulation of neural progenitor cells providing the capacity to proliferate and differentiate into other types of cells. These characteristics make CP epithelial cells (CPECs) excellent candidates for cell therapy aiming at restoring brain tissue in neurodegenerative illnesses, including Alzheimer's disease (AD). In the present study, using in vitro approaches, we demonstrated that CP were able to diminish amyloid-β (Aβ) levels in cell cultures, reducing Aβ-induced neurotoxicity. For in vivo studies, CPECs were transplanted into the brain of the APP/PS1 murine model of AD that exhibits advanced Aβ accumulation and memory impairment. Brain examination after cell implantation revealed a significant reduction in brain Aβ deposits, hyperphosphorylation of tau, and astrocytic reactivity. Remarkably, the transplantation of CPECs was accompanied by a total behavioral recovery in APP/PS1 mice, improving spatial and non-spatial memory. These findings reinforce the neuroprotective potential of CPECs and the use of cell therapies as useful tools in AD.
authors.affiliation: Neuroscience Group, Instituto de Investigacion Hospital 12 de Octubre (i+12), Av. de Córdoba s/n, 28041, Madrid, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Bolos
authors.forename: Desireé
authors.initials: D
authors.lastname: Antequera
authors.forename: Jesús
authors.initials: J
authors.lastname: Aldudo
authors.forename: Henrike
authors.initials: H
authors.lastname: Kristen
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Bullido
authors.forename: Eva
authors.initials: E
authors.lastname: Carro
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology / Q000601:surgery* / Q000628:therapy; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D016380:Brain Tissue Transplantation; D002478:Cells, Cultured; D002831:Choroid Plexus / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016229:Amyloid beta-Peptides