title: Phytochemical analysis and free radical scavenging activity of medicinal plants Gnidia glauca and Dioscorea bulbifera.
abstract: Gnidia glauca and Dioscorea bulbifera are traditional medicinal plants that can be considered as sources of natural antioxidants. Herein we report the phytochemical analysis and free radical scavenging activity of their sequential extracts. Phenolic and flavonoid content were determined. Scavenging activity was checked against pulse radiolysis generated ABTS(•+) and OH radical, in addition to DPPH, superoxide and hydroxyl radicals by biochemical methods followed by principal component analysis. G. glauca leaf extracts were rich in phenolic and flavonoid content. Ethyl acetate extract of D. bulbifera bulbs and methanol extract of G. glauca stem exhibited excellent scavenging of pulse radiolysis generated ABTS(•+) radical with a second order rate constant of 2.33 × 10(6) and 1.72 × 10(6), respectively. Similarly, methanol extract of G. glauca flower and ethyl acetate extract of D. bulbifera bulb with second order rate constants of 4.48 × 10(6) and 4.46 × 10(6) were found to be potent scavengers of pulse radiolysis generated OH radical. G. glauca leaf and stem showed excellent reducing activity and free radical scavenging activity. HPTLC fingerprinting, carried out in mobile phase, chloroform: toluene: ethanol (4: 4: 1, v/v) showed presence of florescent compound at 366 nm as well as UV active compound at 254 nm. GC-TOF-MS analysis revealed the predominance of diphenyl sulfone as major compound in G. glauca. Significant levels of n-hexadecanoic acid and octadecanoic acid were also present. Diosgenin (C₂₇H₄₂O₃) and diosgenin (3á,25R) acetate were present as major phytoconstituents in the extracts of D. bulbifera. G. glauca and D. bulbifera contain significant amounts of phytochemicals with antioxidative properties that can be exploited as a potential source for herbal remedy for oxidative stress induced diseases. These results rationalize further investigation in the potential discovery of new natural bioactive principles from these two important medicinal plants.
authors.affiliation: Institute of Bioinformatics and Biotechnology, University of Pune, Pune, India.
authors.forename: Sougata
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Institute of Bioinformatics and Biotechnology, University of Pune, Pune, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Derle
authors.affiliation: Institute of Bioinformatics and Biotechnology, University of Pune, Pune, India.
authors.forename: Mehul
authors.initials: M
authors.lastname: Ahire
authors.affiliation: Institute of Bioinformatics and Biotechnology, University of Pune, Pune, India.
authors.forename: Piyush
authors.initials: P
authors.lastname: More
authors.affiliation: Institute of Bioinformatics and Biotechnology, University of Pune, Pune, India.
authors.forename: Soham
authors.initials: S
authors.lastname: Jagtap
authors.affiliation: National Centre For Free Radical Research, Department of Chemistry, University of Pune, Pune, India.
authors.forename: Suvarna D
authors.initials: SD
authors.lastname: Phadatare
authors.affiliation: Garware Research Centre, Department of Chemistry, University of Pune, Pune, India.
authors.forename: Ajay B
authors.initials: AB
authors.lastname: Patil
authors.affiliation: Garware Research Centre, Department of Chemistry, University of Pune, Pune, India.
authors.forename: Amit M
authors.initials: AM
authors.lastname: Jabgunde
authors.affiliation: National Centre For Free Radical Research, Department of Chemistry, University of Pune, Pune, India.
authors.forename: Geeta K
authors.initials: GK
authors.lastname: Sharma
authors.affiliation: Garware Research Centre, Department of Chemistry, University of Pune, Pune, India.
authors.forename: Vaishali S
authors.initials: VS
authors.lastname: Shinde
authors.affiliation: Department of Microbiology, University of Pune, Pune, India.
authors.forename: Karishma
authors.initials: K
authors.lastname: Pardesi
authors.affiliation: Garware Research Centre, Department of Chemistry, University of Pune, Pune, India.
authors.forename: Dilip D
authors.initials: DD
authors.lastname: Dhavale
authors.affiliation: Institute of Bioinformatics and Biotechnology, University of Pune, Pune, India ; Department of Microbiology, University of Pune, Pune, India.
authors.forename: Balu A
authors.initials: BA
authors.lastname: Chopade
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D027742:Dioscorea / Q000737:chemistry*; D047309:Flavones / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry*; D019800:Phenol / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D025341:Principal Component Analysis
chemical_list: D000975:Antioxidants; D047309:Flavones; D016166:Free Radical Scavengers; D010936:Plant Extracts; D019800:Phenol