title: Detection and identification of Fabavirus species by one-step RT-PCR and multiplex RT-PCR.
abstract: The genus Fabavirus of the family Secoviridae comprises a group of poorly characterized viruses. To date, only five species have been described: Broad bean wilt virus 1 (BBWV-1), Broad bean wilt virus 2 (BBWV-2), Lamium mild mosaic virus (LMMV), Gentian mosaic virus (GeMV) and Cucurbit mild mosaic virus (CuMMV). The development is described of two RT-PCR procedures for the detection and identification of Fabavirus species: a one-step RT-PCR using a single pair of conserved primers for the detection of all fabaviruses, and a one-step multiplex RT-PCR using species-specific primers for the simultaneous detection and identification of the above-mentioned species of the genus Fabavirus. These methods were applied successfully to field samples and the results were compared with those obtained by molecular hybridization and ELISA. The combination of the two techniques enables rapid, sensitive and reliable identification of the five known fabavirus species, as well as the possibility of discovering new species of this genus.
authors.affiliation: Department of Agricultural and Forestry Sciences, University of Palermo, 90128 Palermo, Italy; Istituto Euromediterraneo di Scienza e Tecnologia (IEMEST), Via E. Amari 123, 90139 Palermo, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Panno
authors.affiliation: Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Ferriol
authors.affiliation: Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia, Spain.
authors.forename: Ezequiel A
authors.initials: EA
authors.lastname: Rangel
authors.affiliation: Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Olmos
authors.affiliation: Department of Plant Pathology, China Agricultural University, Beijing 100193, China.
authors.forename: Cheng-Gui
authors.initials: CG
authors.lastname: Han
authors.affiliation: Department of Agricultural and Forestry Sciences, University of Palermo, 90128 Palermo, Italy.
authors.forename: Federico
authors.initials: F
authors.lastname: Martinelli
authors.affiliation: Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Rubio
authors.affiliation: Department of Agricultural and Forestry Sciences, University of Palermo, 90128 Palermo, Italy; Istituto Euromediterraneo di Scienza e Tecnologia (IEMEST), Via E. Amari 123, 90139 Palermo, Italy. Electronic address: salvatore.davino@unipa.it.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Davino
mesh_terms: D017931:DNA Primers / Q000235:genetics; D019181:Fabavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010935:Plant Diseases / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D017931:DNA Primers; D012367:RNA, Viral
keywords: Conserved primers; Detection; Diagnosis