title: Proteomic analysis and qRT-PCR verification of temperature response to Arthrospira (Spirulina) platensis.
abstract: Arthrospira (Spirulina) platensis (ASP) is a representative filamentous, non-N2-fixing cyanobacterium that has great potential to enhance the food supply and possesses several valuable physiological features. ASP tolerates high and low temperatures along with highly alkaline and salty environments, and can strongly resist oxidation and irradiation. Based on genomic sequencing of ASP, we compared the protein expression profiles of this organism under different temperature conditions (15°C, 35°Cand 45°C) using 2-DE and peptide mass fingerprinting techniques. A total of 122 proteins having a significant differential expression response to temperature were retrieved. Of the positively expressed proteins, the homologies of 116 ASP proteins were found in Arthrospira (81 proteins in Arthrospira platensis str. Paraca and 35 in Arthrospira maxima CS-328). The other 6 proteins have high homology with other microorganisms. We classified the 122 differentially expressed positive proteins into 14 functions using the COG database, and characterized their respective KEGG metabolism pathways. The results demonstrated that these differentially expressed proteins are mainly involved in post-translational modification (protein turnover, chaperones), energy metabolism (photosynthesis, respiratory electron transport), translation (ribosomal structure and biogenesis) and carbohydrate transport and metabolism. Others proteins were related to amino acid transport and metabolism, cell envelope biogenesis, coenzyme metabolism and signal transduction mechanisms. Results implied that these proteins can perform predictable roles in rendering ASP resistance against low and high temperatures. Subsequently, we determined the transcription level of 38 genes in vivo in response to temperature and identified them by qRT-PCR. We found that the 26 differentially expressed proteins, representing 68.4% of the total target genes, maintained consistency between transcription and translation levels. The remaining 12 genes showed inconsistent protein expression with transcription level and accounted for 31.6% of the total target genes.
authors.affiliation: Institute of Biomedical Informatics/Zhejiang Provincial Key Laboratory of Medical Genetics, School of Life Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Huili
authors.affiliation: Institute of Biomedical Informatics/Zhejiang Provincial Key Laboratory of Medical Genetics, School of Life Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Xiaokai
authors.affiliation: Institute of Biomedical Informatics/Zhejiang Provincial Key Laboratory of Medical Genetics, School of Life Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Meili
authors.affiliation: Department of Land, Air and Water Resources, University of California Davis, Davis, California, United States of America.
authors.forename: Randy A
authors.initials: RA
authors.lastname: Dahlgren
authors.affiliation: Institute of Biomedical Informatics/Zhejiang Provincial Key Laboratory of Medical Genetics, School of Life Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wei
authors.affiliation: Institute of Biomedical Informatics/Zhejiang Provincial Key Laboratory of Medical Genetics, School of Life Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Jaiopeng
authors.affiliation: Institute of Biomedical Informatics/Zhejiang Provincial Key Laboratory of Medical Genetics, School of Life Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Chengyang
authors.affiliation: Institute of Biomedical Informatics/Zhejiang Provincial Key Laboratory of Medical Genetics, School of Life Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Chunlei
authors.affiliation: Institute of Biomedical Informatics/Zhejiang Provincial Key Laboratory of Medical Genetics, School of Life Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Yi
authors.affiliation: School of Environmental Sciences and Public Health, Wenzhou Medical University, Wenzhou, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Xuedong
authors.affiliation: Institute of Biomedical Informatics/Zhejiang Provincial Key Laboratory of Medical Genetics, School of Life Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Li
authors.affiliation: Institute of Biomedical Informatics/Zhejiang Provincial Key Laboratory of Medical Genetics, School of Life Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Bao
authors.initials: B
authors.lastname: Qiyu
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D018869:Heat-Shock Response / Q000502:physiology*; D040901:Proteomics; D053545:Spirulina / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins