title: [Application of throat swab nested PCR in the diagnosis of congenital human cytomegalovirus infection in neonates].
abstract: OBJECTIVE
  To evaluate the clinical application value of throat swab nested PCR for detecting active congenital human cytomegalovirus (HCMV) infection in neonates.
  
  
  METHODS
  The throat swabs and umbilical cord blood specimens from 51 neonates were collected for nested PCR assay for HCMV glycoprotein B (gB) gene. Moreover, 18 of them were subjected to a pp65 antigen test.
  
  
  RESULTS
  The sensitivity and specificity of throat swab nested PCR for HCMV gB gene were 67% and 75%, respectively, and the positive and negative predictive values were 57% and 82%, respectively.
  
  
  CONCLUSIONS
  Throat swab nested PCR assay for HCMV gB gene is non-invasive, rapid, and highly sensitive for HCMV detection and holds promise as an excellent screening technology for detecting active congenital HCMV infection in neonates.
authors.affiliation: Department of Pediatrics, Puren Hospital Affiliated to Wuhan University of Science and Technology, Wuhan 430081, China. xiaotingwang0604@sina.com.
authors.forename: Xiao-Ting
authors.initials: XT
authors.lastname: Wang
authors.forename: Zhi-Bing
authors.initials: ZB
authors.lastname: Dong
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Luo
authors.forename: Min
authors.initials: M
authors.lastname: Deng
mesh_terms: D003586:Cytomegalovirus Infections / Q000151:congenital* / Q000175:diagnosis*; D005312:Fetal Blood / Q000821:virology; D006801:Humans; D007231:Infant, Newborn; D010750:Phosphoproteins / Q000097:blood; D016133:Polymerase Chain Reaction / Q000379:methods*; D014759:Viral Envelope Proteins / Q000235:genetics; D014763:Viral Matrix Proteins / Q000097:blood
chemical_list: D010750:Phosphoproteins; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; C040495:glycoprotein B, Simplexvirus; C464963:pp67 protein, human cytomegalovirus