title: Does enhancement of oxidative stress markers mediate health effects of ambient air particles?
abstract: Evidence indicates that oxidative stress generation may contribute to health effects associated with particulate matter (PM) exposure. We investigated oxidative stress markers in 113 workers exposed to metal-rich PM and 61 nonexposed comparable volunteers. The plasma levels of soluble NOX2-derived peptide (sNOX2-dp) and two oxidative stress markers, urinary 8-hydroxydeoxyguanosine (8-OHdG) and 8-iso-prostaglandinF2alpha (8-iso-PGF2α), were analyzed. The plasma levels of the antioxidant alpha-tocopherol were also evaluated. The workers' average exposure to PM with an aerodynamic diameter <10 μm (PM₁₀) was much higher at the workplace than in the city, where the volunteers lived. Workers had significantly higher urinary 8-iso-PGF2α and 8-OHdG and plasma sNOX2-dp levels than nonexposed subjects. Alpha-tocopherol was much lower in workers compared with nonexposed subjects. In multivariable regression models adjusted for age, body mass index, and smoking, 8-iso-PGF2α increased in workers in association with PM10 and metal exposure; 8-OHdG and sNOX2-dp were associated only with iron. Alpha-tocopherol was inversely associated with each of the oxidative stress markers. Our observation leads to the hypothesis that the enhancement of oxidative stress markers associated with exposure to high metal-rich PM levels represents a possible step in the pathways leading from particle exposure to systemic (e.g., cardiovascular) effects.
authors.affiliation: 1 Department of Clinical Sciences and Community Health, Center of Molecular and Genetic Epidemiology, Università di Milano and Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico , Milano, Italy .
authors.forename: Pier Alberto
authors.initials: PA
authors.lastname: Bertazzi
authors.forename: Laura
authors.initials: L
authors.lastname: Cantone
authors.forename: Pasquale
authors.initials: P
authors.lastname: Pignatelli
authors.forename: Laura
authors.initials: L
authors.lastname: Angelici
authors.forename: Valentina
authors.initials: V
authors.lastname: Bollati
authors.forename: Matteo
authors.initials: M
authors.lastname: Bonzini
authors.forename: Michele
authors.initials: M
authors.lastname: Carugno
authors.forename: Pier Mannuccio
authors.initials: PM
authors.lastname: Mannucci
authors.forename: Francesco
authors.initials: F
authors.lastname: Violi
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D015415:Biomarkers / Q000097:blood* / Q000652:urine; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D015237:Dinoprost / Q000031:analogs & derivatives / Q000652:urine; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D018384:Oxidative Stress / Q000187:drug effects*; D052638:Particulate Matter / Q000633:toxicity*; D012044:Regression Analysis; D055815:Young Adult; D024502:alpha-Tocopherol / Q000097:blood
chemical_list: D015415:Biomarkers; D052638:Particulate Matter; C075750:8-epi-prostaglandin F2alpha; D000080242:8-Hydroxy-2'-Deoxyguanosine; D015237:Dinoprost; D003849:Deoxyguanosine; D024502:alpha-Tocopherol