title: [Determination of cinobufagin and resibufogenin in liver tissue by HPLC-MS/MS].
abstract: OBJECTIVE
  To develop a sensitive and accurate assay for detecting cinobufagin and resibufogenin in liver tissue using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).
  
  
  METHODS
  The homogenization of liver tissue with internal standard dexamethasone was extracted with dichloromethane. The extracts with methanol were purified through ProElut C18 solid phase extraction and tested in positive electrospray ionization with multiple reaction monitoring of HPLC-MS/MS.
  
  
  RESULTS
  The good linear relationship of cinobufagin and resibufogenin in liver tissue were 1-204 ng/g and 1-206 ng/g, respectively. The minimal detection threshold (S/N > or = 3) of this method was 0.3 ng/g for both cinobufagin and resibufogenin. The matrix effect was 96.5%-126.7%. The extraction recovery coefficient was 70.0%-82.3%. The precision of intra-day and inter-day was less than 10%.
  
  
  CONCLUSIONS
  This method is sensitive and reliable, and can be used in forensic toxicological analysis.
authors.affiliation: West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
authors.forename: Ming-Dong
authors.initials: MD
authors.lastname: Liu
authors.affiliation: West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: You-Yi
authors.initials: YY
authors.lastname: Yan
authors.affiliation: West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ye
authors.affiliation: West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
authors.forename: Jun-Hong
authors.initials: JH
authors.lastname: Zhao
authors.affiliation: West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xiong
authors.affiliation: West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Min
authors.initials: M
authors.lastname: Xiao
authors.affiliation: West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Lin-Chuan
authors.initials: LC
authors.lastname: Liao
mesh_terms: D002018:Bufanolides / Q000032:analysis* / Q000506:poisoning; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D053593:Forensic Toxicology; D006801:Humans; D008099:Liver / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D002018:Bufanolides; D012997:Solvents; C005734:bufogenin; C002471:cinobufagin