title: QTL mapping for salt tolerance and domestication-related traits in Vigna marina subsp. oblonga, a halophytic species.
abstract: QTL mapping in F2 population [V. luteola × V. marina subsp. oblonga] revealed that the salt tolerance in V. marina subsp. oblonga is controlled by a single major QTL. The habitats of beach cowpea (Vigna marina) are sandy beaches in tropical and subtropical regions. As a species that grows closest to the sea, it has potential to be a gene source for breeding salt-tolerant crops. We reported here for the first time, quantitative trait loci (QTLs) mapping for salt tolerance in V. marina. A genetic linkage map was constructed from an F2 population of 120 plants derived from an interspecific cross between V. luteola and V. marina subsp. oblonga. The map comprised 150 SSR markers. The markers were clustered into 11 linkage groups spanning 777.6 cM in length with a mean distance between the adjacent markers of 5.59 cM. The F2:3 population was evaluated for salt tolerance under hydroponic conditions at the seedling and developmental stages. Segregation analysis indicated that salt tolerance in V. marina is controlled by a few genes. Multiple interval mapping consistently identified one major QTL which can explain about 50% of phenotypic variance. The flanking markers may facilitate transfer of the salt tolerance allele from V. marina subsp. oblonga into related Vigna crops. The QTL for domestication-related traits from V. marina are also discussed.
authors.affiliation: Program in Plant Breeding, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, 73140, Thailand.
authors.forename: Sompong
authors.initials: S
authors.lastname: Chankaew
authors.forename: Takehisa
authors.initials: T
authors.lastname: Isemura
authors.forename: Ken
authors.initials: K
authors.lastname: Naito
authors.forename: Eri
authors.initials: E
authors.lastname: Ogiso-Tanaka
authors.forename: Norihiko
authors.initials: N
authors.lastname: Tomooka
authors.forename: Prakit
authors.initials: P
authors.lastname: Somta
authors.forename: Akito
authors.initials: A
authors.lastname: Kaga
authors.forename: Duncan A
authors.initials: DA
authors.lastname: Vaughan
authors.forename: Peerasak
authors.initials: P
authors.lastname: Srinives
mesh_terms: D001947:Breeding; D002874:Chromosome Mapping; D018744:DNA, Plant / Q000235:genetics; D007887:Fabaceae / Q000235:genetics*; D005819:Genetic Markers; D018895:Microsatellite Repeats; D010641:Phenotype; D040641:Quantitative Trait Loci; D055051:Salt-Tolerant Plants / Q000235:genetics*; D036226:Seedlings / Q000235:genetics
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers