title: Precisely tunable engineering of sub-30 nm monodisperse oligonucleotide nanoparticles.
abstract: Advancement of RNAi therapies is mainly hindered by the development of efficient delivery vehicles. The ability to create small size (<30 nm) oligonucleotide nanoparticles is essential for many aspects of the delivery process but is often overlooked. In this report, we describe diblock star polymers that can reproducibly complex double-stranded oligonucleotides into monodisperse nanoparticles with 15, 23, or 30 nm in diameter. The polymer-nucleic acid nanoparticles have a core-shell architecture with dense PEG brush coating. We characterized these nanoparticles using ITC, DLS, FRET, FCS, TIRF, and TEM. In addition to small size, these nanoparticles have neutral zeta-potentials, making the presented polymer architecture a very attractive platform for investigation of yet poorly studied polyplex size range for siRNA and antisense oligonucleotide delivery applications.
authors.affiliation: Department of Pharmacology, ‡Department of Molecular and Cellular Biology, §Center for Drug Discovery, Dan L. Duncan Cancer Center, and Cardiovascular Research Institute, Baylor College of Medicine , Houston, Texas 77030, United States.
authors.forename: Antons
authors.initials: A
authors.lastname: Sizovs
authors.forename: Xianzhou
authors.initials: X
authors.lastname: Song
authors.forename: M Neal
authors.initials: MN
authors.lastname: Waxham
authors.forename: Yilong
authors.initials: Y
authors.lastname: Jia
authors.forename: Fude
authors.initials: F
authors.lastname: Feng
authors.forename: Jianwei
authors.initials: J
authors.lastname: Chen
authors.forename: Amanda C
authors.initials: AC
authors.lastname: Wicker
authors.forename: Jianming
authors.initials: J
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
mesh_terms: D057005:Bioengineering; D002151:Calorimetry; D002470:Cell Survival; D006367:HeLa Cells; D006801:Humans; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D011092:Polyethylene Glycols