title: Genotypic characterization of Streptococcus pneumoniae serotype 19F in Malaysia.
abstract: Streptococcus pneumoniae is an epidemiologically important bacterial pathogen. Recently, we reported the antibiotic susceptibility patterns of a limited collection of pneumococcal isolates in Malaysia with a high prevalence of erythromycin resistant strains. In the present study, 55 of the pneumococcal isolates of serotype 19F were further analysed by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The generated genotypic patterns were then correlated with the antibiograms previously reported. Forty-seven different PFGE profiles (PTs) were obtained, showing that the isolates were genetically diverse. MLST identified 16 sequence types (STs) with ST-236 being predominant (58.2%), followed by ST-81 (10.3%). Among the ST-236 isolates, 22 were erythromycin resistant S. pneumoniae (ERSP) and 15 were trimethoprim/sulfamethoxazole (TMP/SMX) resistant, while among ST-81, four isolates were ERSP and two were TMP/SMX resistant. The high prevalence of erythromycin resistant serotype 19F isolates of ST-236 in this study has also been reported in other North and South East Asian countries.
authors.affiliation: Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia. Electronic address: jjayakayatri@yahoo.com.
authors.forename: Jayakayatri
authors.initials: J
authors.lastname: Jeevajothi Nathan
authors.affiliation: Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia. Electronic address: mnasir@upm.edu.my.
authors.forename: Mohd Nasir
authors.initials: MN
authors.lastname: Mohd Desa
authors.affiliation: Division of Microbiology, Institute of Biological Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; Biomedical Science and Molecular Typing Laboratory, Institute of Graduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Kwai Lin
authors.initials: KL
authors.lastname: Thong
authors.affiliation: Infectious Disease Epidemiology Group, Sir Henry Wellcome Laboratories, Academic Unit of Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, UK; NIHR Southampton Respiratory Biomedical Research Unit, Southampton, UK; Institute of Life Sciences, University of Southampton, UK; Public Health England, Southampton, UK.
authors.forename: Stuart C
authors.initials: SC
authors.lastname: Clarke
authors.affiliation: Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia. Electronic address: sitinorbaya@upm.edu.my.
authors.forename: Siti Norbaya
authors.initials: SN
authors.lastname: Masri
authors.affiliation: Specialized Diagnostic Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia.
authors.forename: Rohani
authors.initials: R
authors.lastname: Md Yasin
authors.affiliation: Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia. Electronic address: niazlin@upm.edu.my.
authors.forename: Niazlin
authors.initials: N
authors.lastname: Mohd Taib
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000032:analysis; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D008296:Malaysia / Q000453:epidemiology; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D011008:Pneumococcal Infections / Q000453:epidemiology* / Q000382:microbiology*; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial
keywords: Antibiogram; Multilocus sequence type; Pulsed field gel electrophoresis; Serotype 19F; Streptococcus pneumoniae