title: Enhanced abiotic and biotic contributions to dechlorination of pentachlorophenol during Fe(III) reduction by an iron-reducing bacterium Clostridium beijerinckii Z.
abstract: A novel Fe(III) reducing bacterium, Clostridium beijerinckii Z, was isolated from glucose amended paddy slurries, and shown to dechlorinate pentachlorophenol (PCP). Fifty percent of added PCP was removed by C. beijerinckii Z alone, which increased to 83% in the presence of both C. beijerinckii Z and ferrihydrite after 11 days of incubation. Without C. beijerinckii Z, the surface-bound Fe(II) also abiotically dechlorinated more than 40% of the added PCP. This indicated that the biotic dechlorination by C. beijerinckii Z is a dominant process causing PCP transformation through anaerobic dechlorination, and that the dechlorination rates can be accelerated by simultaneous reduction of Fe(III). A biochemical electron transfer coupling process between sorbed Fe(II) produced by C. beijerinckii Z and reductive dehalogenation is a possible mechanism. This finding increases our knowledge of the role of Fe(III) reducing genera of Clostridium in dechlorinating halogenated organic pollutants, such as PCP, in anaerobic paddy soils.
authors.affiliation: College of Environmental and Natural Resource Sciences, Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: College of Environmental and Natural Resource Sciences, Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition, Zhejiang University, Hangzhou 310058, China. Electronic address: yhe2006@zju.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: He
authors.affiliation: College of Environmental and Natural Resource Sciences, Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Feng
authors.affiliation: Experiment Teaching Center for Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Luyi
authors.initials: L
authors.lastname: Liang
authors.affiliation: College of Environmental and Natural Resource Sciences, Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition, Zhejiang University, Hangzhou 310058, China. Electronic address: jmxu@zju.edu.cn.
authors.forename: Jianming
authors.initials: J
authors.lastname: Xu
authors.affiliation: College of Environmental and Natural Resource Sciences, Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition, Zhejiang University, Hangzhou 310058, China.
authors.forename: Philip C
authors.initials: PC
authors.lastname: Brookes
authors.affiliation: College of Environmental and Natural Resource Sciences, Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wu
mesh_terms: D000693:Anaerobiosis; D001483:Base Sequence; D001673:Biodegradation, Environmental; D046970:Clostridium beijerinckii / Q000235:genetics / Q000378:metabolism*; D005296:Ferrous Compounds / Q000378:metabolism; D054879:Halogenation; D006540:Herbicides / Q000378:metabolism*; D008969:Molecular Sequence Data; D010416:Pentachlorophenol / Q000378:metabolism*
chemical_list: D005296:Ferrous Compounds; D006540:Herbicides; D010416:Pentachlorophenol
keywords: Clostridium beijerinckii; Dechlorination; Dissimilatory Fe(III) reducing bacteria; Pentachlorophenol; Sorbed-Fe(II)