title: Lipid rafts and Fas/FasL pathway may involve in elaidic acid-induced apoptosis of human umbilical vein endothelial cells.
abstract: Our previous study showed that trans-fatty acids can cause apoptosis of endothelial cells through the caspase pathway and the mitochondrial pathway. The objective of this study was to explore how trans-fatty acids activate the caspase pathway, whether there exist specific receptors induced apoptosis by comparing normal cells and non-rafts cells treated with elaidic acid (9t18:1) and oleic acid (9c18:1), respectively. Compared to normal cells treated with 9t18:1, the cell viability increased by 13% and the number of apoptotic cells decreased by 3% in non-rafts cells treated with 9t18:1 (p < 0.05), and the expression levels of pro-apoptotic proteins such as caspase-3, -8, -9, Bax, and Bid decreased, and expression of antiapoptotic protein Bcl-2 increased (p < 0.05). In addition, Fas/FasL expression in cell membrane decreased significantly (p < 0.05). In conclusion, the lipid rafts and Fas/FasL pathway may involve in 9t18:1-induced apoptosis of human umbilical vein endothelial cells.
authors.affiliation: State Key Laboratory of Food Science and Technology, Institute for Advanced Study, Nanchang University , Nanchang, Jiangxi 330047, P.R. China.
authors.forename: Huan
authors.initials: H
authors.lastname: Rao
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Ma
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Ze-Yuan
authors.initials: ZY
authors.lastname: Deng
authors.forename: Ya-Wei
authors.initials: YW
authors.lastname: Fan
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D002470:Cell Survival; D053222:Fas Ligand Protein / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D021962:Membrane Microdomains / Q000378:metabolism*; D019301:Oleic Acid / Q000737:chemistry / Q000378:metabolism*; D009829:Oleic Acids; D015398:Signal Transduction; D019014:fas Receptor / Q000378:metabolism*
chemical_list: C491901:FAS protein, human; D053222:Fas Ligand Protein; D009829:Oleic Acids; D019014:fas Receptor; D019301:Oleic Acid; C011459:elaidic acid; D053148:Caspase 3; D053181:Caspase 8
keywords: apoptosis; human umbilical vein endothelial cells; lipid raft; receptor; trans-fatty acid