title: Use of SNAREs for the immobilization of poly-3-hydroxyalkanoate polymerase type II of Pseudomonas putida CA-3 in secretory vesicles of Saccharomyces cerevisiae ATCC 9763.
abstract: Polyhydroxyalkanoate (PHA) synthase, the key enzyme in polyester biosynthesis of bacteria, has been targeted to various organelles in yeasts and plants using respective signal peptides. Here, we report that the sequences derived from SNARE domains efficiently target and integrate the PHA synthase from Pseudomonas putida CA-3 to the membrane of secretory vesicles in Saccharomyces cerevisiae. The studies with the enhanced green fluorescent protein confirm the localization of synthase enzyme in the vesicles of S. cerevisiae.
authors.affiliation: Department of Biotechnology and Bioengineering, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México, D.F., Mexico.
authors.forename: Gurusamy
authors.initials: G
authors.lastname: Muniasamy
authors.affiliation: Department of Biotechnology and Bioengineering, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México, D.F., Mexico; Nanoscience & Nanotechnology Program, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México, D.F., Mexico. Electronic address: fermin@cinvestav.mx.
authors.forename: Fermín
authors.initials: F
authors.lastname: Pérez-Guevara
mesh_terms: D000217:Acyltransferases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D001665:Binding Sites; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018613:Microscopy, Confocal; D016958:Pseudomonas putida / Q000201:enzymology*; D050600:SNARE Proteins / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D022142:Secretory Vesicles / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D050600:SNARE Proteins; D049452:Green Fluorescent Proteins; D000217:Acyltransferases; C077225:poly(3-hydroxyalkanoic acid) synthase
keywords: Enhanced green fluorescent protein; PHA synthase; Polyhydroxyalkanoate; SNARE; Vesicles