title: Complementary studies detecting classical bovine spongiform encephalopathy infectivity in jejunum, ileum and ileocaecal junction in incubating cattle.
abstract: Recently we have described the distribution of bovine spongiform encephalopathy (BSE) infectivity and/or PrPSc in Peyer's patches (PP) of the small intestine of orally BSE infected cattle. In this follow-up study additional jejunal and ileal PP's and ileocaecal-junction tissue samples from 1, 4, and 24 months post infection (mpi) were examined by mouse (Tgbov XV) bioassay. Infectivity was demonstrated in ileal PP's 4 mpi and the distribution/extent of infectivity at 24 mpi was comparable to those seen at earlier time points, revealing no indication for a decline/clearance. These data are relevant for the definition of Specified Risk Materials in the context of the TSE legislation worldwide.
authors.forename: Christine
authors.initials: C
authors.lastname: Fast
authors.forename: Markus
authors.initials: M
authors.lastname: Keller
authors.forename: Anne
authors.initials: A
authors.lastname: Balkema-Buschmann
authors.forename: Bob
authors.initials: B
authors.lastname: Hills
authors.affiliation: Friedrich-Loeffler-Institut, Institute of Novel and Emerging Infectious Diseases, Südufer 10, 17493 Greifswald-Insel Riems, Germany. martin.groschup@fli.bund.de.
authors.forename: Martin H
authors.initials: MH
authors.lastname: Groschup
mesh_terms: D000818:Animals; D002417:Cattle; D002432:Cecum / Q000473:pathology*; D016643:Encephalopathy, Bovine Spongiform / Q000175:diagnosis / Q000473:pathology* / Q000635:transmission; D005260:Female; D005500:Follow-Up Studies; D007082:Ileum / Q000473:pathology*; D007583:Jejunum / Q000473:pathology*; D008822:Mice, Transgenic; D010581:Peyer's Patches / Q000473:pathology*; D018620:PrPSc Proteins / Q000032:analysis*; D018570:Risk Assessment
chemical_list: D018620:PrPSc Proteins