title: [Observation of the pollen mother cell meiosis of male sterile line in rice by using confocal microscopy].
abstract: In the present paper, meiosis of male sterile pollen mother cells and fertility of ripened pollen was observed in male sterile line (Xindao97200A) by using laser scanning confocal microscopy (LSCM), beside, the seed setting rate of selfing and artificial pollination was investigated. Abnormal chromosome behaviors during the meiosis of PMC were found with 15.8%, which included lagging chromosome, dragging chromosomes, three poles of spindles, chromosome bridge, micronuclei and asynchrony of the dyad. Typical, spherical and stained abortive rates were 6.15%, 10.26% and 80.77%, respectively. The pollen abortion might be attributed to abnormal pollen development, which resulted from chromosome pairing abnormally, abnormal division, chromosome breakage and unevenly distributed cytoplasm.
authors.affiliation: Henan Provincial Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, China. jiajia08205635@163.com
authors.forename: Jia-jia
authors.initials: JJ
authors.lastname: Zhang
authors.affiliation: Henan Provincial Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Xi-mei
authors.initials: XM
authors.lastname: Dai
authors.affiliation: Henan Provincial Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Shuai-peng
authors.initials: SP
authors.lastname: Zhao
authors.affiliation: Henan Provincial Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: He-mei
authors.initials: HM
authors.lastname: Liu
authors.affiliation: Henan Provincial Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Qun-ce
authors.initials: QC
authors.lastname: Huang
authors.affiliation: Henan Provincial Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Jiao
mesh_terms: D008540:Meiosis; D018613:Microscopy, Confocal; D012275:Oryza; D051479:Plant Infertility; D011058:Pollen / Q000166:cytology*; D013234:Stem Cells