title: Myeloperoxidase-dependent LDL modifications in bloodstream are mainly predicted by angiotensin II, adiponectin, and myeloperoxidase activity: a cross-sectional study in men.
abstract: The present paradigm of atherogenesis proposes that low density lipoproteins (LDLs) are trapped in subendothelial space of the vascular wall where they are oxidized. Previously, we showed that oxidation is not restricted to the subendothelial location. Myeloperoxidase (MPO), an enzyme secreted by neutrophils and macrophages, can modify LDL (Mox-LDL) at the surface of endothelial cells. In addition we observed that the activation of the endothelial cells by angiotensin II amplifies this process. We suggested that induction of the NADPH oxidase complex was a major step in the oxidative process. Based on these data, we asked whether there was an independent association, in 121 patients, between NADPH oxidase modulators, such as angiotensin II, adiponectin, and levels of circulating Mox-LDL. Our observations suggest that the combination of blood angiotensin II, MPO activity, and adiponectin explains, at least partially, serum Mox-LDL levels.
authors.affiliation: Laboratory of Experimental Medicine (ULB 222 Unit), CHU de Charleroi, A. Vésale Hospital, Université Libre de Bruxelles, Rue de Gozée 706, 6110 Montigny-le-Tilleul, Belgium.
authors.forename: Karim Zouaoui
authors.initials: KZ
authors.lastname: Boudjeltia
authors.affiliation: Laboratory of Pharmaceutical Chemistry and Analytical Platform, Faculty of Pharmacy, Université Libre de Bruxelles, Boulevard du Triomphe, Campus Plaine CP 205/5, 1050 Brussels, Belgium.
authors.forename: Cédric
authors.initials: C
authors.lastname: Delporte
authors.affiliation: Laboratory of Pharmaceutical Chemistry and Analytical Platform, Faculty of Pharmacy, Université Libre de Bruxelles, Boulevard du Triomphe, Campus Plaine CP 205/5, 1050 Brussels, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Van Antwerpen
authors.affiliation: Center for Oxygen Research and Development, Institute of Chemistry B6a, University of Liège, 4000 Liège, Belgium.
authors.forename: Thierry
authors.initials: T
authors.lastname: Franck
authors.affiliation: Center for Oxygen Research and Development, Institute of Chemistry B6a, University of Liège, 4000 Liège, Belgium.
authors.forename: Didier
authors.initials: D
authors.lastname: Serteyn
authors.affiliation: Technology Transfer Office, University of Namur, rue de Bruxelles 61, 5000 Namur, Belgium.
authors.forename: Nicole
authors.initials: N
authors.lastname: Moguilevsky
authors.affiliation: Laboratory of Biochemistry and Cellular Biology (NARILIS), University of Namur, rue de Bruxelles, 61, 5000 Namur, Belgium.
authors.forename: Martine
authors.initials: M
authors.lastname: Raes
authors.affiliation: Institute for Molecular Biology and Medicine (IBMM), Université Libre de Bruxelles, Rue des Professeurs Jeener et Brachet 12, 6041 Gosselies, Belgium.
authors.forename: Luc
authors.initials: L
authors.lastname: Vanhamme
authors.affiliation: Laboratory of Experimental Medicine (ULB 222 Unit), CHU de Charleroi, A. Vésale Hospital, Université Libre de Bruxelles, Rue de Gozée 706, 6110 Montigny-le-Tilleul, Belgium.
authors.forename: Michel
authors.initials: M
authors.lastname: Vanhaeverbeek
authors.affiliation: Laboratory of Experimental Medicine (ULB 222 Unit), CHU de Charleroi, A. Vésale Hospital, Université Libre de Bruxelles, Rue de Gozée 706, 6110 Montigny-le-Tilleul, Belgium.
authors.forename: Alain
authors.initials: A
authors.lastname: Van Meerhaeghe
authors.affiliation: Laboratory of Experimental Medicine (ULB 222 Unit), CHU de Charleroi, A. Vésale Hospital, Université Libre de Bruxelles, Rue de Gozée 706, 6110 Montigny-le-Tilleul, Belgium ; Department of Urology, Erasme University Hospital, Université Libre de Bruxelles, Route de Lennik 808, 1070 Brussels, Belgium.
authors.forename: Thierry
authors.initials: T
authors.lastname: Roumeguère
mesh_terms: D052242:Adiponectin / Q000097:blood*; D000328:Adult; D000368:Aged; D000804:Angiotensin II / Q000097:blood*; D001055:Apolipoproteins B / Q000097:blood; D003430:Cross-Sectional Studies; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood*; D059411:Lower Urinary Tract Symptoms / Q000097:blood; D008297:Male; D008875:Middle Aged; D019255:NADPH Oxidases / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism; D000097423:Peroxidasin
chemical_list: D052242:Adiponectin; D000804:Angiotensin II; D001055:Apolipoproteins B; D008077:Lipoproteins, LDL; D019255:NADPH Oxidases; D009195:Peroxidase; D010544:Peroxidases; C535215:PXDN protein, human; D000097423:Peroxidasin