title: Sequence analysis of the CXCR4 gene derived from cells surviving feline lentivirus infection.
abstract: The feline 3201-S cell line was established from cells that survived productive infection with feline immunodeficiency virus (FIV). We have recently shown that 3201-S cells are free of FIV DNA and are refractory to FIV reinfection. In addition, while the cells express CXCR4, a co-receptor for FIV infection, they are unresponsive to the CXCR4 ligand. In the present study, we show that 3201-S cells encode distinct mutations in the CXCR4 gene. It appears that 3201 cells are heterogeneous, consisting of phenotypically diverse mixed populations resulting from genetic mutations, suggesting that this defect can render the CXCR4 receptor expressed in 3201-S cells biologically dysfunctional.
authors.affiliation: Institute of Laboratory Animals, Graduate School and Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto, 606-8501, Japan, tstochikura@yahoo.co.jp.
authors.forename: Tadafumi S
authors.initials: TS
authors.lastname: Tochikura
authors.forename: Kinjiro
authors.initials: K
authors.lastname: Morimoto
mesh_terms: D000818:Animals; D002415:Cats; D002460:Cell Line; D002470:Cell Survival; D016089:Immunodeficiency Virus, Feline / Q000254:growth & development*; D008969:Molecular Sequence Data; D019718:Receptors, CXCR4 / Q000235:genetics*; D011991:Receptors, Virus / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D019718:Receptors, CXCR4; D011991:Receptors, Virus