title: Roseomonas soli sp. nov., isolated from an agricultural soil cultivated with Chinese cabbage (Brassica campestris).
abstract: A bacterial strain, designated 5N26(T), was isolated from an agricultural soil cultivated with Chinese cabbage (Brassica campestris). Cells of this strain were Gram-reaction-negative, strictly aerobic, motile, non-spore-forming rods, and catalase- and urease-negative. The major fatty acids of strain 5N26(T) were C16 : 0 (7.5 %), C18 : 1 2-OH (13.4 %) and summed feature 8 (C18:1ω6c and/or C18:1ω7c; 63.2%). The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine and one unidentified aminolipid. The G+C content of the genomic DNA of strain 5N26(T) was 68.3 mol%. 16S rRNA gene sequence analysis showed that strain 5N26(T) was phylogenetically related to Roseomonas lacus TH-G33(T) and Roseomonas terrae DS-48(T) (97.0 % and 96.6 % sequence similarity, respectively). The results of genotypic and phenotypic data showed that strain 5N26(T) could be distinguished from phylogenetically related species, and that this strain represented a novel species within the genus Roseomonas, for which the name Roseomonas soli sp. nov. (type strain 5N26(T) = KACC 16376(T) = NBRC 109097(T)) is proposed.
authors.affiliation: Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Dong-Uk
authors.initials: DU
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Jong-Ok
authors.initials: JO
authors.lastname: Ka
mesh_terms: D000383:Agriculture; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001937:Brassica / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D042082:Methylobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2