title: Determination of aroma components in Chinese southwest tobacco by directly suspended droplet microextraction combined with GC-MS.
abstract: In this paper, a simple and efficient approach, directly suspended droplet microextraction (DSDME), has been applied to extract aroma components in Chinese southwest tobacco prior to analysis by gas chromatography-mass spectrometry (GC-MS). The extraction parameters such as organic solvent type, extraction time, temperature, stirring speed and volume were systemically optimized. A single drop of cyclohexane was placed on the top of the aqueous sample which was used as solvent. Under the optimal conditions of DSDME, 62 aroma components of tobacco were analyzed and identified by GC-MS. The approach was used to determine some important aromas in tobacco with the relative recoveries ranged from 75.92 to 102.88%, relative standard deviations in the range of 3.40-7.14% (n = 5) and the limits of detection of 0.0002-0.002 μg/mL. Moreover, the DSDME was applied to identify the aromatic components in Chinese southwest tobacco in this research for the first time and the results suggested that the method can be used as rapid determination of the tobacco. This method can enhance the extracting rate of tobacco aromatic components and meet the need of qualitative analysis of large amount samples.
authors.affiliation: Department of Applied Chemistry, China Agricultural University, 100193 Beijing, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Applied Chemistry, China Agricultural University, 100193 Beijing, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Applied Chemistry, China Agricultural University, 100193 Beijing, China.
authors.forename: Chunzi
authors.initials: C
authors.lastname: Li
authors.affiliation: Tobacco Corporation of Yunnan Dali, #71 Heqing Road, Dali, Yunnan Province 671000, China.
authors.forename: Jinli
authors.initials: J
authors.lastname: Cao
authors.affiliation: Department of Applied Chemistry, China Agricultural University, 100193 Beijing, China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Lai
authors.affiliation: Department of Applied Chemistry, China Agricultural University, 100193 Beijing, China.
authors.forename: Kaixian
authors.initials: K
authors.lastname: Qiu
authors.affiliation: Department of Applied Chemistry, China Agricultural University, 100193 Beijing, China minsg@263.net.
authors.forename: Shungeng
authors.initials: S
authors.lastname: Min
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D003510:Cyclohexanes / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D009812:Odorants / Q000032:analysis*; D015203:Reproducibility of Results; D014026:Nicotiana / Q000737:chemistry*
chemical_list: D003510:Cyclohexanes; C506365:Cyclohexane