title: Tertiary mutations stabilize CD8+ T lymphocyte escape-associated compensatory mutations following transmission of simian immunodeficiency virus.
abstract: Compensatory mutations offset fitness defects resulting from CD8(+) T lymphocyte (CD8(TL))-mediated escape, but their impact on viral evolution following transmission to naive hosts remains unclear. Here, we investigated the reversion kinetics of Gag(181-189)CM9 CD8(TL) escape-associated compensatory mutations in simian immunodeficiency virus (SIV)-infected macaques. Preexisting compensatory mutations did not result in acute-phase escape of the SIVmac239 CD8(TL) epitope Gag(181-189)CM9 and instead required a tertiary mutation for stabilization in the absence of Gag(181-189)CM9 escape mutations. Therefore, transmitted compensatory mutations do not necessarily predict rapid CD8(TL) escape.
authors.affiliation: Vaccine & Gene Therapy Institute, Oregon Health & Science University, Portland, Oregon, USA.
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Burwitz
authors.forename: Helen L
authors.initials: HL
authors.lastname: Wu
authors.forename: Jason S
authors.initials: JS
authors.lastname: Reed
authors.forename: Katherine B
authors.initials: KB
authors.lastname: Hammond
authors.forename: Laura P
authors.initials: LP
authors.lastname: Newman
authors.forename: Benjamin N
authors.initials: BN
authors.lastname: Bimber
authors.forename: Francesca A
authors.initials: FA
authors.lastname: Nimiyongskul
authors.forename: Enrique J
authors.initials: EJ
authors.lastname: Leon
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Maness
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Friedrich
authors.forename: Masaru
authors.initials: M
authors.lastname: Yokoyama
authors.forename: Hironori
authors.initials: H
authors.lastname: Sato
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Matano
authors.forename: David H
authors.initials: DH
authors.lastname: O'Connor
authors.forename: Jonah B
authors.initials: JB
authors.lastname: Sacha
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology; D015683:Gene Products, gag / Q000235:genetics* / Q000276:immunology*; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D009154:Mutation; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000635:transmission / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000276:immunology*
chemical_list: D018984:Epitopes, T-Lymphocyte; D015683:Gene Products, gag