title: Identification and genetic effect of a variable duplication in the promoter region of the cattle ADIPOQ gene.
abstract: In cattle, the ADIPOQ gene is located in the vicinity of the quantitative trait locus (QTL) affecting marbling, the ribeye muscle area and fat thickness on BTA1. In our study, a novel variable duplication (NW_003103812.1:g.9232067_9232133dup) in the bovine ADIPOQ promoter region was identified and genotyped in seven Chinese cattle breeds. Using a reporter assay, we demonstrated that g.9232067_9232133dup decreased the basal transcriptional activity of the ADIPOQ gene in the 3T3-L1 and C2C12 cells. Furthermore, g.9232067_9232133dup suppressed the mRNA expression of the gene in adipose and muscle tissues. An association analysis indicated that the incremental variable duplication was associated with body measurements.
authors.affiliation: College of Animal Science and Technology, Northwest A & F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, 712100, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Yang
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.forename: J
authors.initials: J
authors.lastname: Sun
authors.forename: C
authors.initials: C
authors.lastname: Lei
authors.forename: X
authors.initials: X
authors.lastname: Lan
authors.forename: C
authors.initials: C
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Chen
mesh_terms: D016475:3T3 Cells; D052242:Adiponectin / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics* / Q000254:growth & development; D005786:Gene Expression Regulation; D056726:Genetic Association Studies; D005838:Genotype; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D040641:Quantitative Trait Loci; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D052242:Adiponectin; D012333:RNA, Messenger
keywords: Adiponectin; bovine; growth traits; mRNA expression; promoter activity; variation