title: Understanding thermal adaptation of enzymes through the multistate rational design and stability prediction of 100 adenylate kinases.
abstract: Careful balance between structural stability and flexibility is a hallmark of enzymatic function, and temperature can affect both properties. Canonical (fixed-backbone) enzyme design strategies currently do not consider the role of these properties. Herein, we describe the rational design of 100 temperature-adapted adenylate kinase enzymes using a multistate design strategy that incorporates the impact of conformational changes to backbone structure and stability, in addition to experimental analysis of thermostability and function. Comparison of the experimental temperature of maximum activity to the melting temperature across all 100 variants reveals a strong correlation between these two parameters. In turn, experimental stability data were used to produce accurate predictions of thermostability, providing the requisite complement for de novo temperature-adapted enzyme design. In principle, this level of design-based analysis can be applied to any protein, paving the way toward identifying and understanding the hallmarks of the thermodynamic and structural limits of function.
authors.affiliation: Department of Chemical and Environmental Engineering, Yale University, New Haven, CT 06520-8286, USA.
authors.forename: Stanley C
authors.initials: SC
authors.lastname: Howell
authors.affiliation: Department of Chemical and Environmental Engineering, Yale University, New Haven, CT 06520-8286, USA.
authors.forename: Krishna Kishore
authors.initials: KK
authors.lastname: Inampudi
authors.affiliation: Department of Chemical and Environmental Engineering, Yale University, New Haven, CT 06520-8286, USA.
authors.forename: Doyle P
authors.initials: DP
authors.lastname: Bean
authors.affiliation: Department of Chemical and Environmental Engineering, Yale University, New Haven, CT 06520-8286, USA; Department of Biomedical Engineering, Yale University, New Haven, CT 06520-8286, USA; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8286, USA. Electronic address: corey.wilson@yale.edu.
authors.forename: Corey J
authors.initials: CJ
authors.lastname: Wilson
mesh_terms: D000263:Adenylate Kinase / Q000737:chemistry*; D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology*; D002152:Calorimetry, Differential Scanning; D003198:Computer Simulation; D004798:Enzymes / Q000737:chemistry*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012984:Software; D013816:Thermodynamics
chemical_list: D004798:Enzymes; D000263:Adenylate Kinase