title: Common glycoproteins expressing polylactosamine-type glycans on matched patient primary and metastatic melanoma cells show different glycan profiles.
abstract: Recently, we reported comparative analysis of glycoproteins which express cancer-specific N-glycans on various cancer cells and identified 24 glycoproteins having polylactosamine (polyLacNAc)-type N-glycans that are abundantly present in malignant cells [ Mitsui et al., J. Pharm. Biomed. Anal. 2012 , 70 , 718 - 726 ]. In the present study, we applied the technique to comparative studies on common glycoproteins present in the matched patient primary and metastatic melanoma cell lines. Metastatic melanoma cells (WM266-4) contained a large amount of polyLacNAc-type N-glycans in comparison with primary melanoma cells (WM115). To identify the glycoproteins expressing these N-glycans, glycopeptides having polyLacNAc-type N-glycans were captured by a Datura stramonium agglutinin (DSA)-immobilized agarose column. The captured glycopeptides were analyzed by LC/MS after removing N-glycans, and some glycoproteins such as basigin, lysosome-associated membrane protein-1 (LAMP-1), and chondroitin sulfate proteoglycan 4 (CSPG4) were identified in both WM115 and WM266-4 cells. The expression level of polyLacNAc of CSPG4 in WM266-4 cells was significantly higher than that in WM115 cells. In addition, sulfation patterns of chondroitin sulfate (CS) chains in CSPG4 showed dramatic changes between these cell lines. These data show that characteristic glycans attached to common proteins observed in different stages of cancer cells will be useful markers for determining degree of malignancies of tumor cells.
authors.affiliation: School of Pharmacy, Kinki University , Kowakae 3-4-1, Higashi-Osaka 577-8502, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Kinoshita
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Mitsui
authors.forename: Naotaka
authors.initials: N
authors.lastname: Kakoi
authors.forename: Keita
authors.initials: K
authors.lastname: Yamada
authors.forename: Takao
authors.initials: T
authors.lastname: Hayakawa
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Kakehi
mesh_terms: D000595:Amino Acid Sequence; D000606:Amino Sugars / Q000737:chemistry*; D015153:Blotting, Western; D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000606:Amino Sugars; D006023:Glycoproteins; D011134:Polysaccharides; C066929:polylactosamine