title: Phosphate flow between hybrid histidine kinases CheA₃ and CheS₃ controls Rhodospirillum centenum cyst formation.
abstract: Genomic and genetic analyses have demonstrated that many species contain multiple chemotaxis-like signal transduction cascades that likely control processes other than chemotaxis. The Che₃ signal transduction cascade from Rhodospirillum centenum is one such example that regulates development of dormant cysts. This Che-like cascade contains two hybrid response regulator-histidine kinases, CheA₃ and CheS₃, and a single-domain response regulator CheY₃. We demonstrate that cheS₃ is epistatic to cheA₃ and that only CheS₃∼P can phosphorylate CheY₃. We further show that CheA₃ derepresses cyst formation by phosphorylating a CheS₃ receiver domain. These results demonstrate that the flow of phosphate as defined by the paradigm E. coli chemotaxis cascade does not necessarily hold true for non-chemotactic Che-like signal transduction cascades.
authors.affiliation: Molecular and Cellular Biochemistry Department, Indiana University, Bloomington, Indiana, United States of America.
authors.forename: Kuang
authors.initials: K
authors.lastname: He
authors.affiliation: Molecular and Cellular Biochemistry Department, Indiana University, Bloomington, Indiana, United States of America.
authors.forename: Jeremiah N
authors.initials: JN
authors.lastname: Marden
authors.affiliation: Department of Biology, Indiana University, Bloomington, Indiana, United States of America.
authors.forename: Ellen M
authors.initials: EM
authors.lastname: Quardokus
authors.affiliation: Molecular and Cellular Biochemistry Department, Indiana University, Bloomington, Indiana, United States of America.
authors.forename: Carl E
authors.initials: CE
authors.lastname: Bauer
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002633:Chemotaxis / Q000235:genetics; D003560:Cysts / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins; D000071677:Histidine Kinase; D008565:Membrane Proteins / Q000235:genetics; D000072236:Methyl-Accepting Chemotaxis Proteins; D010710:Phosphates / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D042182:Rhodospirillum centenum / Q000201:enzymology*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins; D010710:Phosphates; C000607606:cheY protein, E coli; D011494:Protein Kinases; D000071677:Histidine Kinase; C000607608:cheA protein, E coli