title: Transcriptomic study of the red palm weevil Rhynchophorus ferrugineus embryogenesis.
abstract: The red palm weevil (RPW), Rhynchophorus ferrugineus (Coleoptera: Curculionidae), is an invasive, concealed and destructive tissue borer, and it becomes a lethal pest of the palm family of plants and has been reported to attack 20 palm species around the globe. Here we report a systematic transcriptomic study on embryogenesis of RPW, where we analyze the transcriptomes across five developmental stages of RPW embryogenesis, involving four embryonic stages (E1, E2, E3 and E4) and one larval stage (L1). Using the RNA-seq and next-generation platforms, we generated 80 to 91 million reads for each library and assemble 22 532 genes that are expressed at different embryonic stages. Among the total transcripts from the five embryonic development stages, we found that 30.45 % are differentially expressed, 10.10 % show stage-specificity and even a larger fraction, 62.88 %, exhibit constitutive expression in all the stages. We also analyzes the expression dynamics of several conserved signaling pathways (such as Hedgehog, JAK-STAT, Notch, TGF-β, Ras/MAPK and Wnt), as well as key developmental genes, including those related to apoptosis, axis formation, Hox complex, neurogenesis and segmentation. The datasets provide an essential resource for gene annotation and RPW functional genomics, including studies by using tools and concepts from multiple disciplines, such as development, physiology, biochemistry, molecular biology and genetics.
authors.affiliation: Joint Center for Genomics Research (JCGR), King Abdulaziz City for Science and Technology (KACST) and Chinese Academy of Sciences (CAS), Riyadh, Kingdom of Saudi Arabia; CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China.
authors.forename: An
authors.initials: A
authors.lastname: Yin
authors.forename: Linlin
authors.initials: L
authors.lastname: Pan
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Yin
authors.forename: Shangang
authors.initials: S
authors.lastname: Jia
authors.forename: Wanfei
authors.initials: W
authors.lastname: Liu
authors.forename: Chengqi
authors.initials: C
authors.lastname: Xin
authors.forename: Kan
authors.initials: K
authors.lastname: Liu
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Yu
authors.forename: Gaoyuan
authors.initials: G
authors.lastname: Sun
authors.forename: Khalid
authors.initials: K
authors.lastname: Al-hudaib
authors.forename: Songnian
authors.initials: S
authors.lastname: Hu
authors.forename: Ibrahim S
authors.initials: IS
authors.lastname: Al-Mssallem
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
mesh_terms: D000818:Animals; D028023:Arecaceae / Q000469:parasitology; D001483:Base Sequence; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D015723:Gene Library; D049750:Genome, Insect; D007814:Larva / Q000235:genetics; D017421:Sequence Analysis; D015398:Signal Transduction / Q000235:genetics; D014158:Transcription, Genetic; D048889:Weevils / Q000196:embryology / Q000235:genetics* / Q000254:growth & development
keywords: RNA-seq; Rhynchophorus ferrugineus; insect embryogenesis; red palm weevil; signaling pathways; transcriptome