title: The use of eugenol against Aeromonas hydrophila and its effect on hematological and immunological parameters in silver catfish (Rhamdia quelen).
abstract: The aim of this study was to evaluate the activity of eugenol against the fish pathogen Aeromonas hydrophila and eugenol's effect on hematological and natural immune parameters in silver catfish (Rhamdia quelen). In vitro, eugenol showed weak activity against A. hydrophila, but in vivo, at a subinhibitory concentration (10 mg L(-1)), it promoted survival in infected silver catfish. Eugenol (50 μg mL(-1)) reduced the hemolytic activity of A. hydrophila supernatant in vitro in fish erythrocytes. Subjecting catfish to eugenol baths (5 and 10 mg L(-1)) for five days did not alter the hematological and immunological parameters studied in this work. Based on these results, eugenol can be used to treat or prevent bacterial diseases in fish.
authors.affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Fernando Jonas
authors.initials: FJ
authors.lastname: Sutili
authors.affiliation: Faculdade de Agronomia e Medicina Veterinária, Universidade de Passo Fundo, 99052-900 Passo Fundo, RS, Brazil.
authors.forename: Luiz Carlos
authors.initials: LC
authors.lastname: Kreutz
authors.affiliation: Faculdade de Agronomia e Medicina Veterinária, Universidade de Passo Fundo, 99052-900 Passo Fundo, RS, Brazil.
authors.forename: Mirela
authors.initials: M
authors.lastname: Noro
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Leticia Trevisan
authors.initials: LT
authors.lastname: Gressler
authors.affiliation: Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Berta Maria
authors.initials: BM
authors.lastname: Heinzmann
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Agueda Castagna
authors.initials: AC
authors.lastname: de Vargas
authors.affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil. Electronic address: bbaldisserotto@hotmail.com.
authors.forename: Bernardo
authors.initials: B
authors.lastname: Baldisserotto
mesh_terms: D016980:Aeromonas hydrophila / Q000187:drug effects*; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002397:Catfishes / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D005054:Eugenol / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D005054:Eugenol
keywords: Aeromonas; Bacteria; Fish; Hematology; Innate immune system; Silver catfish