title: Preparation and antibacterial activity of oligosaccharides derived from dandelion.
abstract: In this study, we prepared oligosaccharides from dandelion (Taraxacum officinale) by hydrolysis with hydrogen peroxide (H2O2) and investigated their antibacterial activity. The optimum hydrolysis conditions, as determined using the response surface methodology, were as follows: reaction time, 5.12h; reaction temperature, 65.53 °C and H2O2 concentration, 3.16%. Under these conditions, the maximum yield of the oligosaccharides reached 25.43%. The sugar content in the sample was 96.8%, and the average degree of polymerisation was approximately 9. The oligosaccharides showed high antibacterial activity against Escherichia coli, Bacillus subtilis and Staphylococcus aureus, indicating that dandelion-derived oligosaccharides have the potential to be used as antibacterial agents.
authors.affiliation: Microbiology Laboratory, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China. Electronic address: qianlilyg@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Qian
authors.affiliation: Microbiology Laboratory, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China. Electronic address: zhoulygsh@sina.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Geriatrics, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China.
authors.forename: Zhaolin
authors.initials: Z
authors.lastname: Teng
authors.affiliation: Department of Nursing, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China.
authors.forename: Chun-Ling
authors.initials: CL
authors.lastname: Du
authors.affiliation: Geriatrics, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China.
authors.forename: Changrong
authors.initials: C
authors.lastname: Tian
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D009844:Oligosaccharides / Q000737:chemistry / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects; D031205:Taraxacum / Q000737:chemistry*; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D009844:Oligosaccharides; D006861:Hydrogen Peroxide
keywords: Antibacterial activities; Dandelion-derived oligosaccharides; Hydrogen peroxide