title: High glucose-induced barrier impairment of human retinal pigment epithelium is ameliorated by treatment with Goji berry extracts through modulation of cAMP levels.
abstract: Human retinal pigment epithelium cells were used to investigate the mechanisms underlying blood-retinal barrier disruption under conditions of chronic hyperglycemia. The treatment with 25 mM glucose caused a rapid drop in the transepithelial electrical resistance (TEER), which was reversed by the addition of either a methanolic extract from Goji (Lycium barbarum L.) berries or its main component, taurine. Intracellular cAMP levels increased concurrently with the high glucose-induced TEER decrease, and were correlated to an increased activity of the cytosolic isoform of the enzyme adenylyl cyclase. The treatment with plant extract or taurine restored control levels. Data are discussed in view of a possible prevention approach for diabetic retinopathy.
authors.affiliation: Dept of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy. Electronic address: pvnbbr@unife.it.
authors.forename: Barbara
authors.initials: B
authors.lastname: Pavan
authors.affiliation: Dept of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Capuzzo
authors.affiliation: Dept of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Forlani
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism*; D017097:Electric Impedance; D005638:Fruit; D005947:Glucose / Q000633:toxicity*; D006801:Humans; D032305:Lycium / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D055213:Retinal Pigment Epithelium / Q000187:drug effects* / Q000378:metabolism; D013654:Taurine / Q000494:pharmacology; D019108:Tight Junctions / Q000187:drug effects*
chemical_list: D010936:Plant Extracts; D013654:Taurine; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D005947:Glucose
keywords: HRPE cells; TEER; adenylyl cyclase; high glucose; plant extract