title: Ex vivo cultures of glioblastoma in three-dimensional hydrogel maintain the original tumor growth behavior and are suitable for preclinical drug and radiation sensitivity screening.
abstract: Identification of new drugs and predicting drug response are major challenges in oncology, especially for brain tumors, because total surgical resection is difficult and radiation therapy or chemotherapy is often ineffective. With the aim of developing a culture system close to in vivo conditions for testing new drugs, we characterized an ex vivo three-dimensional culture system based on a hyaluronic acid-rich hydrogel and compared it with classical two-dimensional culture conditions. U87-MG glioblastoma cells and seven primary cell cultures of human glioblastomas were subjected to radiation therapy and chemotherapy drugs. It appears that 3D hydrogel preserves the original cancer growth behavior and enables assessment of the sensitivity of malignant gliomas to radiation and drugs with regard to inter-tumoral heterogeneity of therapeutic response. It could be used for preclinical assessment of new therapies.
authors.affiliation: Aix Marseille Université, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille, France; CRO2, UMR 911, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille Cedex, France; INSERM, U911, 13005 Marseille, France. Electronic address: carine.jiguet-jiglaire@univ-amu.fr.
authors.forename: Carine
authors.initials: C
authors.lastname: Jiguet Jiglaire
authors.affiliation: Aix Marseille Université, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille, France; CRO2, UMR 911, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille Cedex, France; INSERM, U911, 13005 Marseille, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Baeza-Kallee
authors.affiliation: Aix Marseille Université, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille, France; CRO2, UMR 911, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille Cedex, France; INSERM, U911, 13005 Marseille, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Denicolaï
authors.affiliation: Aix Marseille Université, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille, France; CRO2, UMR 911, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille Cedex, France; INSERM, U911, 13005 Marseille, France.
authors.forename: Doriane
authors.initials: D
authors.lastname: Barets
authors.affiliation: Aix Marseille Université, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille, France; CRO2, UMR 911, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille Cedex, France; INSERM, U911, 13005 Marseille, France; APHM, Timone Hospital, Department of Neurosurgery, 13005 Marseille, France; Timone Hospital, 264 Rue Saint Pierre, 13385 Marseille Cedex 5, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Metellus
authors.affiliation: Aix Marseille Université, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille, France; CRO2, UMR 911, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille Cedex, France; INSERM, U911, 13005 Marseille, France; Timone Hospital, 264 Rue Saint Pierre, 13385 Marseille Cedex 5, France; APHM, Timone Hospital, Department of Radiotherapy, 13005 Marseille, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Padovani
authors.affiliation: Aix Marseille Université, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille, France; CRO2, UMR 911, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille Cedex, France; INSERM, U911, 13005 Marseille, France; Timone Hospital, 264 Rue Saint Pierre, 13385 Marseille Cedex 5, France; APHM, Timone Hospital, Department of Neurooncology, 13005 Marseille, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Chinot
authors.affiliation: Aix Marseille Université, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille, France; CRO2, UMR 911, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille Cedex, France; INSERM, U911, 13005 Marseille, France; Timone Hospital, 264 Rue Saint Pierre, 13385 Marseille Cedex 5, France; APHM, Timone Hospital, Department of Pathology, 13005 Marseille, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Figarella-Branger
authors.affiliation: Aix Marseille Université, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille, France; CRO2, UMR 911, Faculté de Médecine de la Timone, 27 boulevard Jean Moulin, 13284 Marseille Cedex, France; INSERM, U911, 13005 Marseille, France; Timone Hospital, 264 Rue Saint Pierre, 13385 Marseille Cedex 5, France; APHM, Timone Hospital, Department of Pathology, 13005 Marseille, France.
authors.forename: Carla
authors.initials: C
authors.lastname: Fernandez
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D048430:Cell Shape; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005909:Glioblastoma / Q000473:pathology*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D051379:Mice; D008819:Mice, Nude; D061251:Primary Cell Culture / Q000379:methods*; D011836:Radiation Tolerance; D054457:Tissue Scaffolds / Q000737:chemistry; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays / Q000379:methods
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
keywords: 3D environment; Glioblastoma; Preclinical tool; Sensitivity screening