title: Performance of an automated multiplex immunofluorescence assay for detection of Chlamydia trachomatis immunoglobulin G.
abstract: Chlamydia serology is indicated to investigate etiology of miscarriage, infertility, pelvic inflammatory disease, and ectopic pregnancy. Here, we assessed the reliability of a new automated-multiplex immunofluorescence assay (InoDiag test) to detect specific anti-C. trachomatis immunoglobulin G. Considering immunofluorescence assay (IF) as gold standard, InoDiag tests exhibited similar sensitivities (65.5%) but better specificities (95.1%-98%) than enzyme-linked immunosorbent assays (ELISAs). InoDiag tests demonstrated similar or lower cross-reactivity rates when compared to ELISA or IF.
authors.affiliation: Center for Research on Intracellular Bacteria, Institute of Microbiology, Faculty of Biology and Medicine, University of Lausanne and University Hospital Center (CHUV), Lausanne, Switzerland; Departments of Obstetrics and Gynecology, Materno-fetal and Obstetrics Research Unit, University Hospital Center (CHUV), Lausanne, Switzerland.
authors.forename: David
authors.initials: D
authors.lastname: Baud
authors.affiliation: Departments of Obstetrics and Gynecology, Materno-fetal and Obstetrics Research Unit, University Hospital Center (CHUV), Lausanne, Switzerland.
authors.forename: Jade
authors.initials: J
authors.lastname: Zufferey
authors.affiliation: Departments of Obstetrics and Gynecology, Materno-fetal and Obstetrics Research Unit, University Hospital Center (CHUV), Lausanne, Switzerland.
authors.forename: Patrick
authors.initials: P
authors.lastname: Hohlfeld
authors.affiliation: Center for Research on Intracellular Bacteria, Institute of Microbiology, Faculty of Biology and Medicine, University of Lausanne and University Hospital Center (CHUV), Lausanne, Switzerland. Electronic address: gilbert.greub@chuv.ch.
authors.forename: Gilbert
authors.initials: G
authors.lastname: Greub
mesh_terms: D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D012698:Serologic Tests
chemical_list: D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic
keywords: Chlamydia trachomatis; Diagnostic test; Intracellular bacteria; Serology