title: Anti-inflammatory and anti-osteoporotic lignans from Vitex negundo seeds.
abstract: Chemical investigation of Vitex negundo seeds afforded four new lignans, including a phenylindene-type lignan, vitexdoin F (1), and three phenylnaphthalene-type lignans, vitexdoins G, H and I (2-4). Their structures were elucidated by detailed spectroscopic analyses on the basis of NMR, IR, and MS data. All compounds were evaluated for their anti-inflammatory and anti-osteoporotic activities, employing RAW264.7 macrophages, osteoblast-like UMR106 and osteoclastic cells, respectively. Compound 1 showed significant inhibition on the nitric oxide (NO) production (IC50 4.17 μg/mL) due to its down-regulation of the inducible nitric oxide synthase (iNOS) protein expression in LPS-stimulated RAW264.7 cells, which also exhibited potent stimulatory effects on the proliferation and ALP (alkaline phosphatase) activity of UMR106 cells, and significantly up-regulated the OPG/RANKL protein ratio.
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, PR China.
authors.forename: Cheng-Jian
authors.initials: CJ
authors.lastname: Zheng
authors.affiliation: Department of Pharmacy, No. 107 Hospital of PLA, 7 South Zhichu Road, Yantai 264002, PR China.
authors.forename: Xiang-Wang
authors.initials: XW
authors.lastname: Zhang
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Han
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, PR China.
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Jiang
authors.affiliation: Center for Drug Evaluation, China Food and Drug Administration, No. Jia 1 Fuxing Road, Beijing 100038, PR China. Electronic address: dr.jytang@yahoo.com.cn.
authors.forename: Jian-Yuan
authors.initials: JY
authors.lastname: Tang
authors.affiliation: Department of Oral Biological and Medical Sciences, University of British Columbia, 2350 Health Science Mall, Rm 4558, Vancouver, BC V6T1Z3, Canada.
authors.forename: Dieter
authors.initials: D
authors.lastname: Brömme
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, PR China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai 200433, PR China. Electronic address: qinsmmu@126.com.
authors.forename: Lu-Ping
authors.initials: LP
authors.lastname: Qin
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification*; D001172:Arthritis, Rheumatoid / Q000188:drug therapy*; D050071:Bone Density Conservation Agents / Q000737:chemistry / Q000302:isolation & purification*; D002460:Cell Line; D004353:Drug Evaluation, Preclinical; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry; D020950:Vitex / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D050071:Bone Density Conservation Agents; D017705:Lignans; D010936:Plant Extracts
keywords: Anti-inflammatory; Anti-osteoporotic; Vitex negundo; Vitexdoin