title: Regeneration of full-thickness skin defects using umbilical cord blood stem cells loaded into modified porous scaffolds.
abstract: In this study, we have demonstrated the ability of cord blood (CB)-derived unrestricted somatic stem cells (USSCs) and chitosan-modified poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) scaffold to promote skin regeneration. Afterward, the scaffolds were evaluated by structural, microscopic, physical, and mechanical assays and cell culture analyses. Results of structural, physical, and mechanical analyses also showed a good resilience and compliance with movement as a skin graft. Cellular experiments showed a better cell adhesion, growth, and proliferation inside the modified scaffolds compared with unmodified ones. In animal models with histological examinations, all groups, excluding the control group especially the groups treated with stem cells, exhibited the most pronounced effect on wound closure, with the statistically significant improvement in wound healing being seen at postoperative day 21. These data suggest that chitosan-modified PHBV scaffold loaded with CB-derived USSCs could significantly contribute to wound repair and be potentially used in the tissue engineering.
authors.affiliation: From the *Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran; †Department of Biomaterial Engineering, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran; ‡Proteomics Research Center, Shahid Beheshti University of Medical Science, Tehran, Iran; and §Tissue Engineering Department, School of Advanced Technologies in Medicine, Tehran University of Medical Science, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Zeinali
authors.forename: Esmaeil
authors.initials: E
authors.lastname: Biazar
authors.forename: Saeed Heidari
authors.initials: SH
authors.lastname: Keshel
authors.forename: Mostafa Rezaei
authors.initials: MR
authors.lastname: Tavirani
authors.forename: Kamal
authors.initials: K
authors.lastname: Asadipour
mesh_terms: D000818:Animals; D048271:Chitosan; D005312:Fetal Blood / Q000166:cytology; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D008297:Male; D011091:Polyesters; D051381:Rats; D017208:Rats, Wistar; D016038:Skin Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D000094122:Polyhydroxybutyrates
chemical_list: D048271:Chitosan; D011091:Polyesters; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates