title: Bacillus bingmayongensis sp. nov., isolated from the pit soil of Emperor Qin's Terra-cotta warriors in China.
abstract: A Bacillus-like isolate, strain FJAT-13831(T), isolated from the No. 1 pit soil of Emperor Qin's Terra-cotta Warriors in Xi'an City, China, was studied to determine its taxonomic status. Dominant fatty acids of this organism included iso-C15:0, iso-C17:0, C16:0, iso-C13:0, anteiso-C15:0, and iso-C17:1ω5c. Comparative 16S rRNA gene sequence analysis confirmed the affiliation of this isolate to the genus Bacillus and indicated that it was closely related to Bacillus pseudomycoides DSM 12442(T) (99.72 % similarity). A phylogenetic analysis of the gyrB gene sequence similarities exhibited independent clustering of the isolate FJAT-13831(T) and showed 93.8 % (<95 %) sequence similarity with its closest phylogenetic neighbour B. pseudomycoides DSM 12442(T). Separate standing of the strain FJAT-13831(T) was supported by a whole genome-based phylogenetic analysis with an average nucleotide identity value of 91.47 (<95 %) between isolate FJAT-13831(T) and B. pseudomycoides DSM 12442(T) and was consistent with the results of DNA-DNA hybridization (69.1 % relatedness). These findings support the conclusion that the isolate FJAT-13831(T) represents a novel species, for which the name Bacillus bingmayongensis sp. nov. is proposed. The type strain is FJAT-13831(T) (= CGMCC 1.12043(T) = DSM 25427(T)).
authors.affiliation: Agricultural Bio-Resource Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, Fujian, People's Republic of China, fzliubo@163.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Guo-Hong
authors.initials: GH
authors.lastname: Liu
authors.forename: Gui-Ping
authors.initials: GP
authors.lastname: Hu
authors.forename: Cetin
authors.initials: C
authors.lastname: Sengonca
authors.forename: Sengonca
authors.initials: S
authors.lastname: Cetin
authors.forename: Nai-Quan
authors.initials: NQ
authors.lastname: Lin
authors.forename: Jian-Yang
authors.initials: JY
authors.lastname: Tang
authors.forename: Wei-Qi
authors.initials: WQ
authors.lastname: Tang
authors.forename: Ying-Zhi
authors.initials: YZ
authors.lastname: Lin
mesh_terms: D001407:Bacillus / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S