title: Cell proliferation arrest and redox state status as part of different stages during senescence establishment in mouse fibroblasts.
abstract: Senescence phenotype can be achieved by multiple pathways. Most of them involve the activation of negative cell cycle regulators as well as a shift to an oxidative status. However, the exact participation of these events in senescence establishment and maintenance is not completely understood. In this study we investigated the content of three final cell cycle regulators, as well as the redox state in some critical points during the pre-senescent and the full-senescent states. Our results highlight the existence of a critical pre-phase in senescent phenotype establishment, in which cell proliferation stops with the participation of the cell cycle inhibitors, and a second maintenance stage where the exacerbated pro-oxidant state inside the cell induces the physiological decline characteristic in senescent cells.
authors.affiliation: Departamento de Ciencias de la Salud, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, A.P. 55-535, 09340, Mexico, DF, Mexico.
authors.forename: Francisco
authors.initials: F
authors.lastname: Triana-Martínez
authors.forename: Norma E
authors.initials: NE
authors.lastname: López-Diazguerrero
authors.forename: Luis A
authors.initials: LA
authors.lastname: Maciel-Barón
authors.forename: Sandra L
authors.initials: SL
authors.lastname: Morales-Rosales
authors.forename: Sonia
authors.initials: S
authors.lastname: Galván-Arzate
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Fernandez-Perrino
authors.forename: Alejandro
authors.initials: A
authors.lastname: Zentella
authors.forename: Viviana I
authors.initials: VI
authors.lastname: Pérez
authors.forename: Luis E
authors.initials: LE
authors.lastname: Gomez-Quiroz
authors.forename: Mina
authors.initials: M
authors.lastname: Königsberg
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D059447:Cell Cycle Checkpoints / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects / Q000502:physiology*; D002839:Chromans / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D051379:Mice; D010084:Oxidation-Reduction; D010641:Phenotype; D050050:Protein Carbonylation
chemical_list: D000975:Antioxidants; D002839:Chromans; D004247:DNA; D005978:Glutathione; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; D019803:Glutathione Disulfide