title: In vivo anti-inflammatory activity of Tabernaemontana divaricata leaf extract on male albino mice.
abstract: OBJECTIVE
  To study the in vivo anti-inflammatory activity of Tabernaemontana divaricata leaf extract on male albino mice.
  
  
  METHODS
  Aqueous decoction and methanol leaf extracts were tested for their ability to reduce croton oil-induced edema in the mouse ear after topical application. The methanol leaf extract dose-dependently inhibited the croton oil-induced ear edema in mice (ID50 <500 μg·cm(-2)). A bioassay-guided liquid-liquid fractionation of this methanol extract gave four active fractions: water insoluble (F1), hexane (F2), ethyl acetate (F3) and water (F4).
  
  
  RESULTS
  The hexane fraction showed a very high activity (42.1% inhibition at 0.7 μg·cm(-2)) as compared to the control. The other fractions were less active (F1: 56.1% at 506.2 μg·cm(-2); F3: 57.3% at 289.3 μg·cm(-2); and F4: 31.9% for 203.8 μg·cm(-2)) while indomethacin gave 48.8% of inhibition at 90 μg·cm(-2). The activity of F1 and F3 may be at least in part explained by the presence of anti-inflammatory flavonoids, while the activity was not correlated to the tannin contents. No compounds were detected in the most active F2 fraction.
  
  
  CONCLUSIONS
  The results give a rational support to the traditional use of T. divaricata in tropical India as anti-inflammatory agent.
authors.affiliation: College of Pharmacy, IPS Academy, Rajendra Nagar, AB Road, Indore MP India 452012. Electronic address: sachin225819@rediffmail.com.
authors.forename: Sachin
authors.initials: S
authors.lastname: Jain
authors.affiliation: College of Pharmacy, IPS Academy, Rajendra Nagar, AB Road, Indore MP India 452012.
authors.forename: Praveen
authors.initials: P
authors.lastname: Sharma
authors.affiliation: College of Pharmacy, IPS Academy, Rajendra Nagar, AB Road, Indore MP India 452012.
authors.forename: Santosh
authors.initials: S
authors.lastname: Ghule
authors.affiliation: College of Pharmacy, IPS Academy, Rajendra Nagar, AB Road, Indore MP India 452012.
authors.forename: Ankit
authors.initials: A
authors.lastname: Jain
authors.affiliation: Department of Pharmacy, Oriental University, Indore MP India 452001.
authors.forename: Nitesh
authors.initials: N
authors.lastname: Jain
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D004487:Edema / Q000188:drug therapy*; D006801:Humans; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage*; D018515:Plant Leaves / Q000737:chemistry*; D030014:Tabernaemontana / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts
keywords: Anti-inflammatory; Mouse ear edema; Plant extract; Quercitrin; Tabernaemontana divaricata; Traditional medicine