title: Proteomic analysis and immune properties of exosomes released by macrophages infected with Mycobacterium avium.
abstract: The role of exosomes shed from Mycobacterium avium sp. paratuberculosis-infected macrophages in intercellular communication processes was examined. We compared the responses of resting macrophages infected with M. avium sp. paratuberculosis with those of resting macrophages treated with exosomes previously released from macrophages infected with M. avium sp. paratuberculosis. Some proteins components of exosomes released from resting macrophages infected with M. avium sp. paratuberculosis showed a significantly differential expression compared with exosomes from uninfected-macrophages. Both M. avium sp. paratuberculosis and exosomes from infected-cells enhanced the expression of CD80 and CD86 and the secretion of TNF-α and IFN-γ by macrophages. This suggests that exosomes from infected macrophages may be carriers of molecules, e.g. bacterial antigens and/or components from infected macrophages, that can elicit responses in resting cells. Two-dimensional analysis of the proteins present in exosomes from M. avium sp. paratuberculosis-infected macrophages compared with those from resting cells resulted in the identification by MALDI-TOF/TOF mass spectrometry of the following differentially expressed proteins: two actin isoforms, guanine nucleotide-binding protein β-1, cofilin-1 and peptidyl-prolyl cis-trans isomerase A. The possible relevance of the changes observed and the biological functions of the proteins differentially present are discussed.
authors.affiliation: Molecular Biology Research Center, School of Life Science, Central South University, Changsha 410078, People's Republic of China.
authors.forename: Jian-jun
authors.initials: JJ
authors.lastname: Wang
authors.affiliation: Molecular Biology Research Center, School of Life Science, Central South University, Changsha 410078, People's Republic of China.
authors.forename: Cai
authors.initials: C
authors.lastname: Chen
authors.affiliation: Molecular Biology Research Center, School of Life Science, Central South University, Changsha 410078, People's Republic of China.
authors.forename: Ping-fang
authors.initials: PF
authors.lastname: Xie
authors.affiliation: Molecular Biology Research Center, School of Life Science, Central South University, Changsha 410078, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Control Center of Tuberculosis in Hunan Province, Changsha 410205, People's Republic of China.
authors.forename: Yun-hong
authors.initials: YH
authors.lastname: Tan
authors.affiliation: Molecular Biology Research Center, School of Life Science, Central South University, Changsha 410078, People's Republic of China. Electronic address: tljxie@csu.edu.cn.
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Tang
mesh_terms: D000199:Actins / Q000032:analysis; D015703:Antigens, CD / Q000032:analysis; D002460:Cell Line; D021984:Cyclophilin A / Q000032:analysis; D016207:Cytokines / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D055354:Exosomes / Q000737:chemistry* / Q000276:immunology*; D019204:GTP-Binding Proteins / Q000032:analysis; D006801:Humans; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology; D009162:Mycobacterium avium / Q000254:growth & development*; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000199:Actins; D015703:Antigens, CD; D016207:Cytokines; D020543:Proteome; D019204:GTP-Binding Proteins; D021984:Cyclophilin A
keywords: 2-DE; Exosomes; MALDI-TOF/TOF MS; Macrophage; Mycobacterium avium