title: [Determination of the biological attribute of evidence at the scene using reverse transcription PCR and real-time fluorescent quantitative PCR].
abstract: OBJECTIVE
  To explore the feasibility of biological method to identify the biological attribute of samples at crime scene.
  
  
  METHODS
  Thirty samples of ten blood stains, ten saliva stains and ten semen stains were selected, and all the samples were processed by the routine method and biomolecular method, respectively. Both RNA and DNA were isolated using DNA-RNA co-extraction technology and the mRNA was converted into cDNA using reverse transcription PCR (RT-PCR). Three pairs of specific primers were designed for blood stain, saliva stain and semen stain based on the different target genes in three specific tissues and the primers were amplified using real-time fluorescent quantitative PCR. The differences in these biological samples were evaluated by melting temperature (Tm) values and the size of the amplification fragment.
  
  
  RESULTS
  The Tm values of blood stain, saliva stain and semen stain were (84.5 +/- 0.2) degrees C, (76.9 +/- 0.3) degrees C and (88.5 +/- 0.2) degrees C, respectively. The length of PCR fragments of them was 177bp, 134bp and 294bp, respectively.
  
  
  CONCLUSIONS
  Compared with the routine method, RT-PCR with real-time fluorescent quantitative PCR is highly specific, sensitive and reliable to identify the biological attribute of evidence, and can be potentially applied to determine evidence attribute in forensic practice.
authors.affiliation: Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China. xxnnxy@hotmail.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China.
authors.forename: Qing-Wen
authors.initials: QW
authors.lastname: Xu
authors.affiliation: Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China.
authors.forename: Jiang-Ping
authors.initials: JP
authors.lastname: Huang
authors.affiliation: Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Liu
authors.affiliation: Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China.
authors.forename: Kai-Nan
authors.initials: KN
authors.lastname: Zou
authors.affiliation: Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ping
authors.affiliation: Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China.
authors.forename: Huai-Gu
authors.initials: HG
authors.lastname: Zhou
mesh_terms: D001801:Blood Stains; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D017931:DNA Primers; D005554:Forensic Medicine / Q000379:methods*; D005819:Genetic Markers; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012313:RNA / Q000032:analysis* / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis; D012463:Saliva; D012661:Semen; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D005819:Genetic Markers; D012333:RNA, Messenger; D012313:RNA; D004247:DNA