title: Expression of selected genes in liver biopsy specimens in relation to early virological response in patients with chronic hepatitis C with HCV mono- and HIV/HCV co-infection.
abstract: The aim of our study was to evaluate the significance of IL-28B single-nucleotide polymorphism and hepatic expression of IFI27, SOCS3 and miR-122 in order to predict early virological response (EVR) in patients infected with HCV genotype 1 or 4. The study group consisted of 65 patients: 46 with HCV mono- and 19 with HIV/HCV co-infection. Analyses of IL-28B single-nucleotide polymorphism C/T (rs12979860) in the blood and expression of SOCS3, IFI27 and miR-122 in liver biopsy samples obtained before PegIFN and ribavirin treatment were performed by the RT-PCR method. EVR was defined as a >2log decline in HCV viremia at week 12. EVR was associated with a lower expression of IFI27 and a more frequent presence of the IL28BCC genotype. IFI27 expression was lower in patients with the CC genotype, irrespective of EVR. In multivariate logistic regression, only IL28B CC genotype and age above 40 years influenced EVR (OR =5.09 and 0.29 respectively). In contrast to IFI27, expression of miR-122 and SOCS3 in patients with different IL28B genotypes was not statistically significantly different. A correlation between miR-122 and SOCS3 was found (Rho =0.495094 p< 0.0001). Analysis of IFI27, SOCS3 and miR-122 hepatic expression does not provide substantial benefits for the prognosis of EVR. The only independent prognostic factors for EVR are age and IL28B genotype. The prognostic significance of IFI27 expression for EVR is dependent on the genetic polymorphism of IL28B.
authors.affiliation: Department of Infectious Diseases and Hepatology, Medical University of Łódź, Kniaziewicza 1/5, 91-347, Łódź, Poland, elajablonowska@gmail.com.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Jabłonowska
authors.forename: Kamila
authors.initials: K
authors.lastname: Wójcik
authors.forename: Ewa
authors.initials: E
authors.lastname: Koślińska-Berkan
authors.forename: Bożena
authors.initials: B
authors.lastname: Szymańska
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Omulecka
authors.forename: Anna
authors.initials: A
authors.lastname: Piekarska
mesh_terms: D015415:Biomarkers; D001706:Biopsy; D060085:Coinfection / Q000473:pathology*; D020869:Gene Expression Profiling; D015658:HIV Infections / Q000150:complications* / Q000235:genetics / Q000473:pathology*; D019698:Hepatitis C, Chronic / Q000150:complications* / Q000235:genetics / Q000473:pathology*; D006801:Humans; D007372:Interferons; D007378:Interleukins / Q000235:genetics; D008099:Liver / Q000473:pathology*; D008565:Membrane Proteins / Q000096:biosynthesis; D035683:MicroRNAs / Q000096:biosynthesis; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000096:biosynthesis; D000095022:Interferon Lambda
chemical_list: D015415:Biomarkers; D007372:Interferons; D007378:Interleukins; D008565:Membrane Proteins; D035683:MicroRNAs; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; C406887:IFI27 protein, human; C000624353:interferon-lambda, human; D000095022:Interferon Lambda; C531907:MIRN122 microRNA, human; C106644:SOCS3 protein, human