title: Evidences for B6C3-Tg (APPswe/PSEN1dE9) double-transgenic mice between 3 and 10 months as an age-related Alzheimer's disease model.
abstract: Transgenic mouse has shown great advantages in the study of Alzheimer's disease (AD) and drug screening as AD develops rapidly resent years, while more detail information of these transgenic mice and experience of application are needed. To obtain the basic background information of the B6C3-Tg (APPswe/PSEN1dE9) double-transgenic mouse, which was reported with early onset AD, three- to ten-month-old B6C3-Tg AD mice and normal C57BL/6 mice were selected randomly to test the ability of learning memory by Morris water maze, the brain acetylcholinesterase (AChE) activity by AChE kit, and beta amyloid protein level by immunohistochemistry staining. Compared with the control group, the escape latency time of B6C3-Tg AD mice at 9 and 10 months of age is significantly longer (P < 0.05) in Morris maze test, and the activity of brain AChE is higher. β-Amyloid plaques were observed at 3 months of age and developed rapidly. Statistical analysis showed a positive correlation between the area of these plaques and the ages of B6C3-Tg AD mouse (y = 0.0355e(0.5557x), R = 0.9557). The model's behavior is conformed to simulate behaviors of human Alzheimer's disease at the early stage and may provide detail background information a new choice when transgenic mice are needed in the research of AD.
authors.affiliation: Southern Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zhong
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.forename: Xiansheng
authors.initials: X
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Jiarong
authors.initials: J
authors.lastname: Yan
authors.forename: Shenglai
authors.initials: S
authors.lastname: Liu
authors.forename: Xia
authors.initials: X
authors.lastname: Sun
authors.forename: Ke
authors.initials: K
authors.lastname: Liu
authors.forename: Huangquan
authors.initials: H
authors.lastname: Lin
authors.forename: Shaosong
authors.initials: S
authors.lastname: Kuang
authors.forename: Xiaojiang
authors.initials: X
authors.lastname: Tang
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000367:Age Factors; D000544:Alzheimer Disease / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D016564:Amyloid beta-Protein Precursor / Q000235:genetics*; D000818:Animals; D001921:Brain / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology; D008297:Male; D018782:Maze Learning; D051379:Mice; D008810:Mice, Inbred C57BL; D058225:Plaque, Amyloid / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D053764:Presenilin-1 / Q000235:genetics*
chemical_list: D016564:Amyloid beta-Protein Precursor; D053764:Presenilin-1; C580804:presenilin 1, mouse; D000110:Acetylcholinesterase