title: Thidiazuron-induced changes in biomass parameters, total phenolic content, and antioxidant activity in callus cultures of Artemisia absinthium L.
abstract: Callus culture of Artemisia absinthium L. was established for enhanced production of phenolics and higher antioxidant activity. Callus was induced from seed-derived leaf explants, incubated on to MS media supplemented with thidiazuron (TDZ; 0.5-5.0 mg/l) either alone or in combination with α-naphthalene acetic acid (NAA; 1.0 mg/l). These callus cultures were investigated for their growth kinetics, total phenolic content, and antioxidant activity on weekly basis for a period of 49 days. Maximum dry biomass accumulation of 8.73 g/l was observed on day 42 in response to 1.0 mg/l TDZ and 1.0 mg/l NAA. Furthermore, maximum level of total phenolic content of 8.53 mg GAE/g DW and highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of 72.6% were observed in calli formed in response to 1.0 mg/l TDZ on day 42. The results showed a positive correlation of total phenolic content and DPPH radical scavenging activity in most of the callus cultures of A. absinthium L.
authors.affiliation: Department of Biotechnology, Quaid-i-Azam University, Islamabad, 45320, Pakistan.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ali
authors.forename: Bilal Haider
authors.initials: BH
authors.lastname: Abbasi
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D031171:Artemisia absinthium / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D018533:Biomass; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors / Q000737:chemistry; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010636:Phenols / Q000737:chemistry* / Q000302:isolation & purification; D010671:Phenylurea Compounds / Q000494:pharmacology*; D010851:Picrates / Q000037:antagonists & inhibitors / Q000737:chemistry; D018515:Plant Leaves / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D058439:Plant Somatic Embryogenesis Techniques; D013830:Thiadiazoles / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D009280:Naphthaleneacetic Acids; D010636:Phenols; D010671:Phenylurea Compounds; D010851:Picrates; D013830:Thiadiazoles; C016785:thidiazuron; C034182:1-naphthaleneacetic acid; C004931:1,1-diphenyl-2-picrylhydrazyl