title: Quantification of cell co-migration occurrences during cell aggregation on fibroin substrates.
abstract: A quantitative analytical method was proposed for measuring cell co-migration, which was defined as two or more cells migrating together. To accurately identify and quantify this behavior, cell migration on fibroin substrates was analyzed with respect to intercellular distance. Specifically, cell size was characterized by major diameter, and then, based on these measurements and cell center data, a specific threshold distance for defining co-migration was determined after analyzing cell motion using the Voronoi diagram method. The results confirmed that co-migration occurrences of rounded cells were significantly more stable on fibroin than on ProNectin substrates under the present experimental conditions. The cell co-migration analysis method in this article was shown to be successful in evaluating the stability of cell co-migration and also suggested the presence of "critical distance" where two cells interact on fibroin substrates. With further research, the cell co-migration analysis method and "critical distance" may prove to be capable of identifying the aggregation behavior of other cells on different materials, making it a valuable tool that can be used in tissue engineering design.
authors.affiliation: 1 Department of Mechanical Engineering and Science, Graduate School of Engineering, Kyoto University , Kyoto, Japan .
authors.forename: Akihisa
authors.initials: A
authors.lastname: Otaka
authors.forename: Kazuya
authors.initials: K
authors.lastname: Takahashi
authors.forename: Yuji S
authors.initials: YS
authors.lastname: Takeda
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kambe
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Kuwana
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Tamada
authors.forename: Naohide
authors.initials: N
authors.lastname: Tomita
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002465:Cell Movement; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D005349:Fibroins / Q000737:chemistry*; D011817:Rabbits
chemical_list: D005349:Fibroins