title: Isolation and structure elucidation of pentahydroxyscirpene, a trichothecene Fusarium mycotoxin.
abstract: Pentahydroxyscirpene, a novel trichothecene-type compound, was isolated from Fusarium-inoculated rice. The structure of pentahydroxyscirpene was elucidated by 1D and 2D NMR spectroscopy and X-ray single-crystal diffraction. The conformation in solution was determined by NOESY experiments supported by quantum chemical calculations. In vitro toxicity tests showed that pentahydroxyscirpene inhibits protein synthesis as do other trichothecenes.
authors.affiliation: Institute of Applied Synthetic Chemistry, Vienna University of Technology (VUT) , Getreidemarkt 9/163, A-1060 Vienna, Austria.
authors.forename: Philipp
authors.initials: P
authors.lastname: Fruhmann
authors.forename: Hannes
authors.initials: H
authors.lastname: Mikula
authors.forename: Gerlinde
authors.initials: G
authors.lastname: Wiesenberger
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Varga
authors.forename: Daniel
authors.initials: D
authors.lastname: Lumpi
authors.forename: Berthold
authors.initials: B
authors.lastname: Stöger
authors.forename: Georg
authors.initials: G
authors.lastname: Häubl
authors.forename: Marc
authors.initials: M
authors.lastname: Lemmens
authors.forename: Franz
authors.initials: F
authors.lastname: Berthiller
authors.forename: Rudolf
authors.initials: R
authors.lastname: Krska
authors.forename: Gerhard
authors.initials: G
authors.lastname: Adam
authors.forename: Christian
authors.initials: C
authors.lastname: Hametner
authors.forename: Johannes
authors.initials: J
authors.lastname: Fröhlich
mesh_terms: D018360:Crystallography, X-Ray; D005670:Fusarium / Q000737:chemistry*; D008968:Molecular Conformation; D015394:Molecular Structure; D009183:Mycotoxins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D019906:Nuclear Magnetic Resonance, Biomolecular; D012275:Oryza / Q000382:microbiology; D011500:Protein Synthesis Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014255:Trichothecenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D009183:Mycotoxins; D011500:Protein Synthesis Inhibitors; D014255:Trichothecenes; C587676:pentahydroxyscirpene