title: [Effect of salidroside on apoptosis of bone marrow mesenchymal stem cells induced by ara-C].
abstract: The purpose of this study was to investigate the effect of salidroside on human bone marrow mesenchymal stem cell (hBMMSC) apoptosis induced by cytarabine C (Ara-C) and its mechanism, hBMMSC were cultured in vitro and isolated by Fircoll density gradient centrifugation; cell surface antigens were measured by flow cytometry; the osteogenic and adipogenic differentiation of MSC was tested and evaluated by specific staining methods. The proliferation and apoptosis of cells exposed to Ara- C were detected by MTT and flow cytometry respectively. The experiments were divided into 4 groups: control group, Ara-C group, salidroside group and Ara-C+salidroside group. The mRNA expression of BCL-2 and BAX was assayed by RT-PCR. The results showed that the adherent cells displayed spindle and fibroblast cell-like shape; the hBMMSC expressed CD44, CD71 and HLA-ABC, not expressed CD34, CD45 and HLA-DR; the hBMMSC successfully differentiated into osteogenic and adipogenic lineages, which showed mineralization with von Kossa staining. Furthermore, liquid vacuoles were detected by oil red O staining; Ara- C exhibited a less inhibitory effect on the proliferation of hBMMSC treated with salidroside. The apoptosis of hBMMSC treated with salidroside were significantly higher as compared with control group (P < 0.05); RT-PCR results demonstrated that the BCL-2 expression was significantly down regulated but BAX mRNA expressions was up-regulated in Ara- C group as compared with those in the control group. Salidroside significantly inhibited the apoptosis of MSC and reversed the mRNA expression of BCL-2 and BAX. It is concluded that salidroside can inhibit the apoptosis of hBMMSC induced by Ara-C, its mechanism may be related with the regulation of BCL-2/BAX expression.
authors.affiliation: Department of Hematology, Ningxia People's Hospital, Yinchuan 750012, Ningxia Province, China.
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Wei
authors.affiliation: Key Laboratory of Stem cell and Gene Drugs in Gansu Province, Department of Hematology, Lanzhou General Hospital, Lanzhou Military Command, Lanzhou 730050, Gansu Province, China. E-mail:baihai98@torn.corn.
authors.forename: Hai
authors.initials: H
authors.lastname: Bai
authors.affiliation: Department of Hematology, Gansu Provincial People's Hospital, Lanzhou 730050, Gansu Province, China. E-mail:yanqingfang40@163.com.
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Sun
authors.affiliation: Department of Hematology, Ningxia People's Hospital, Yinchuan 750012, Ningxia Province, China.
authors.forename: Shen
authors.initials: S
authors.lastname: Bao
authors.affiliation: Key Laboratory of Stem cell and Gene Drugs in Gansu Province, Department of Hematology, Lanzhou General Hospital, Lanzhou Military Command, Lanzhou 730050, Gansu Province, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xi
authors.affiliation: Key Laboratory of Stem cell and Gene Drugs in Gansu Province, Department of Hematology, Lanzhou General Hospital, Lanzhou Military Command, Lanzhou 730050, Gansu Province, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D002478:Cells, Cultured; D003561:Cytarabine / Q000494:pharmacology; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology*
chemical_list: D005960:Glucosides; D010636:Phenols; D003561:Cytarabine; C009172:rhodioloside