title: Isolation and characterization of peptidoglycan recognition protein 1 from antler base of sika deer (Cervus nippon).
abstract: Peptidoglycan recognition proteins (PGRPs) are secreted innate immunity pattern recognition molecules. In this study, a new peptidoglycan recognition protein 1 named cnPGRP1 was isolated from an antler base of sika deer Cervus nippon. The antler base antimicrobial proteins (AAP) were subjected to consecutive chromatographic methods connected to Sephadex G-25 gel filtration column (CM) anion-exchange column, and RP-HPLC. The molecular weight of cnPGRP1 was 17.2 kDa under SDS-PAGE, and peptide mass fingerprint analysis by MALDI-TOF-MS as peptidoglycan recognition protein 1 matched to Dasypus novemcinctus. The matched amino acids sequences were RLYEIIQKWPHYRA. Both Gram-positive and Gram-negative bacteria can be killed by cnPGRP1 in the 50-250 μg/mL range through in vitro. Furthermore, cnPGRP1 has been found to bind Gram-positive bacteria, Gram-negative bacteria, and even fungus.
authors.affiliation: College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.affiliation: College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, PR China. Electronic address: jlu_jw@163.com.
authors.forename: Yongguang
authors.initials: Y
authors.lastname: Yin
authors.affiliation: College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, PR China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, PR China.
authors.forename: Guidan
authors.initials: G
authors.lastname: He
authors.affiliation: College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Qi
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000999:Antlers / Q000737:chemistry*; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D003670:Deer; D008297:Male
chemical_list: D000890:Anti-Infective Agents; D002352:Carrier Proteins; C118897:peptidoglycan recognition protein
keywords: Antimicrobial activity; Antler base; MALDI-TOF-MS; Peptidoglycan recognition protein 1