title: Novel porphyrazine derivatives show promise for photodynamic therapy despite restrictions in hydrophilicity.
abstract: Complexing of ligands to photosensitizers (Ps) has gained popularity by enhancing solubility, cell-surface recognition and tissue specificity for applications in Photodynamic Therapy (PDT) and fluorescence-based diagnostics. Here we report on nine carbohydrate-functionalized porphyrazine (Pz-galactopyranose/methyl-ribose) derivatives bearing either H2 , Zn(II) or Ni(II) cores for potential use in PDT. Derivatives proved soluble only in organic solvents; dichloromethane (DCM) and tetrahydrofuran (THF). Derivatives were subsequently solubilized using DCM-based PEG-DSPE5000 -PBS encapsulation for biological studies due to THF cytotoxicity. Absorption spectra analyses viewed no correlation between core ion, carbohydrate type and peripheral position though encapsulation efficiency (%EE) followed a general order of Zn(II) (60-92%) > H2 (5-34%) > Ni(II) (4-21%). As such, phototoxicity of Zn(II)Pz derivatives were far superior to H2 Pz and Ni(II)Pz counterparts following 631.4 nm excitation of MCF-7 breast cancer cells. Variation was attributed to persistent aggregation and low %EE when regarding the absorption properties recorded. It is therefore believed that revision of the encapsulation method for H2 Pz and Ni(II)Pz derivatives would render improved phototoxicity. Zn(II)Pz derivatives show promise as agents for PDT of cancer.
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Johannesburg, Auckland Park, 2006, South Africa.
authors.forename: Tamarisk K
authors.initials: TK
authors.lastname: Horne
authors.forename: Marianne J
authors.initials: MJ
authors.lastname: Cronjé
mesh_terms: D057927:Hydrophobic and Hydrophilic Interactions; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry*; D012995:Solubility
chemical_list: D017319:Photosensitizing Agents