title: The influence of nitrogen on the development and accumulation of protein bodies in the developing endosperm of wheat caryopses.
abstract: The aim of the present work was to reveal the histological changes of protein bodies (PBs) in the developing wheat endosperm under nitrogen (N) treatment. For this purpose, the development and accumulation of PBs in the dorsal and ventral regions of wheat endosperm affected by N application at booting stage were investigated using light microscopy and Image-Pro Plus 6.0 software. The endosperm without N treatment contained many smaller PBs that were scattered in endosperm cells in an unordered pattern, whereas the endosperm with N treatment contained many larger PBs or aggregations that were concentrated in a certain region of endosperm cells. The amount and relative areas of PBs in wheat varieties cvs. Xumai 30 and Yangmai 13 were significantly increased by N application. However, the cultivars differed with the degree of response to N being cv. Xumai 30 > cv. Yangmai 13. These differences also varied with position in the endosperm in the order ventral > dorsal region. The initiation of PBs occurred 3 days earlier in N-treated endosperm than the control.
authors.affiliation: Key Laboratories of Crop Genetics and Physiology of the Jiangsu Province and Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou, 225009, China, feixiong@yzu.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Xiong
authors.forename: Xu-Run
authors.initials: XR
authors.lastname: Yu
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Ai-Sheng
authors.initials: AS
authors.lastname: Xiong
mesh_terms: D056625:Endosperm / Q000187:drug effects / Q000378:metabolism*; D009584:Nitrogen / Q000494:pharmacology*; D015388:Organelles / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012639:Seeds / Q000187:drug effects / Q000254:growth & development; D014908:Triticum / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
chemical_list: D010938:Plant Oils; D011506:Proteins; D009584:Nitrogen