title: Ultrasensitive detection of thrombin using surface plasmon resonance and quartz crystal microbalance sensors by aptamer-based rolling circle amplification and nanoparticle signal enhancement.
abstract: The surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) aptasensors combined with rolling circle amplification and bio-bar-coded AuNP enhancement have been applied to detect the human α-thrombin for the first time. The assay platform exhibited excellent selectivity and sensitivity with detection limit as low as 0.78 aM.
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: He
authors.forename: Lijun
authors.initials: L
authors.lastname: Liu
authors.forename: Wenping
authors.initials: W
authors.lastname: Qiao
authors.forename: Shusheng
authors.initials: S
authors.lastname: Zhang
mesh_terms: D052157:Aptamers, Nucleotide; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D058525:Quartz Crystal Microbalance Techniques; D059629:Signal-To-Noise Ratio; D020349:Surface Plasmon Resonance; D013917:Thrombin / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D006046:Gold; D013917:Thrombin