title: Development of solid-phase microextraction to study dissolved organic matter--polycyclic aromatic hydrocarbon interactions in aquatic environment.
abstract: Solid-phase microextraction coupled with gas chromatography and mass spectrometry (SPME-GC-MS) was developed for the study of interactions between polycyclic aromatic hydrocarbons (PAHs) and dissolved organic matter (DOM). After the determination of the best conditions of extraction, the tool was applied to spiked water to calculate the dissolved organic carbon water distribution coefficient (K(DOC)) in presence of different mixtures of PAHs and Aldrich humic acid. The use of deuterated naphthalene as internal standard for freely dissolved PAH quantification was shown to provide more accuracy than regular external calibration. For the first time, K(DOC) values of 18 PAHs were calculated using data from SPME-GC-MS and fluorescence quenching; they were in agreement with the results of previous studies. Competition between PAHs, deuterated PAHs and DOM was demonstrated, pointing out the non-linearity of PAH-DOM interactions and the stronger interactions of light molecular weight PAHs (higher K(DOC) values) in absence of high molecular weight PAHs.
authors.affiliation: Université de Bordeaux, UMR 5805, EPOC-LPTC, 351 Cours de la Libération, Talence Cedex F-33405, France; CNRS, UMR 5805, EPOC-LPTC, F-33405 Talence Cedex, France.
authors.forename: Chloé
authors.initials: C
authors.lastname: de Perre
authors.affiliation: Université de Bordeaux, UMR 5805, EPOC-LPTC, 351 Cours de la Libération, Talence Cedex F-33405, France; CNRS, UMR 5805, EPOC-LPTC, F-33405 Talence Cedex, France.
authors.forename: Karyn
authors.initials: K
authors.lastname: Le Ménach
authors.affiliation: Université de Bordeaux, UMR 5805, EPOC-LPTC, 351 Cours de la Libération, Talence Cedex F-33405, France; CNRS, UMR 5805, EPOC-LPTC, F-33405 Talence Cedex, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Ibalot
authors.affiliation: Université de Bordeaux, UMR 5805, EPOC-LPTC, 351 Cours de la Libération, Talence Cedex F-33405, France; CNRS, UMR 5805, EPOC-LPTC, F-33405 Talence Cedex, France.
authors.forename: Edith
authors.initials: E
authors.lastname: Parlanti
authors.affiliation: Université de Bordeaux, UMR 5805, EPOC-LPTC, 351 Cours de la Libération, Talence Cedex F-33405, France; CNRS, UMR 5805, EPOC-LPTC, F-33405 Talence Cedex, France. Electronic address: h.budzinski@epoc.u-bordeaux1.fr.
authors.forename: Hélène
authors.initials: H
authors.lastname: Budzinski
mesh_terms: D003903:Deuterium / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000592:standards; D006812:Humic Substances; D006863:Hydrogen-Ion Concentration; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000302:isolation & purification / Q000592:standards; D012015:Reference Standards; D052617:Solid Phase Microextraction; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D005456:Fluorescent Dyes; D006812:Humic Substances; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D003903:Deuterium
keywords: Aquatic environment; Dissolved organic matter; Gas chromatography–mass spectrometry; Interactions; Polycyclic aromatic hydrocarbons; Solid-phase microextraction