title: Changes in monoclonal HLA-DR antigen expression in acute organophosphorus pesticide-poisoned patients.
abstract: The aim of this study was to investigate changes in human leukocyte antigen (HLA)-DR expression of peripheral blood mononuclear cells (MNCs) in patients with acute organophosphorus pesticide poisoning (AOPP). HLA-DR antigen expression of peripheral blood MNCs was examined in 75 patients with AOPP, including 36 patients without multiple organ dysfunction syndrome (non-MODS) and 39 patients with multiple organ dysfunction syndrome (MODS), as well as in 30 healthy individuals using flow cytometry assay. The associations between HLA-DR antigen expression and certain parameters were analyzed, including acute physiology and chronic health evaluation II (APACHE II) score, serum cholinesterase (ChE) activity, cardiac troponin I (cTnI), cardiac enzymes, and liver and kidney function. The mean fluorescence intensity (MCF) of HLA-DR expression in the AOPP group (21.59±5.36) was significantly lower than that in the control group (27.85±4.86) (P<0.001). The MCF in the MODS group (18.17±4.23) was lower than that in the non-MODS group (25.15±6.15). In addition, the MCF of the deceased patients (15.29±3.97) was lower than that of the surviving patients (22.34±2.76) (P<0.001). The MCF of patients with AOPP and MODS was positively correlated with serum ChE (P<0.01) and negatively correlated with the APACHE II score, creatine kinase isoenzyme, cTnI, lactate dehydrogenase, alanine aminotransferase, aspartate aminotransferase, blood urea nitrogen and serum creatinine (P<0.05). In conclusion, HLA-DR expression in patients with AOPP was significantly decreased compared with that in healthy individuals; HLA-DR expression may therefore be a good indicator for evaluating AOPP, MODS disease severity, immune function, efficacy of prognosis and prognosis. Examination of HLA-DR antigen expression may be of crucial clinical value.
authors.affiliation: Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Chenyun
authors.initials: C
authors.lastname: Xia
authors.affiliation: Department of Nephropathy, People's Hospital of Peking University, Beijing 100044, P.R. China.
authors.forename: Mi
authors.initials: M
authors.lastname: Wang
authors.affiliation: Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liang
authors.affiliation: Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Ling'an
authors.initials: L
authors.lastname: Yun
authors.affiliation: Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Housheng
authors.initials: H
authors.lastname: Kang
authors.affiliation: Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Fan
authors.affiliation: Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Wang
authors.affiliation: Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Guoyuan
authors.initials: G
authors.lastname: Zhang
keywords: acute organophosphate pesticide poisoning; human leukocyte antigen-DR; multiple organ dysfunction syndrome