title: Vascularized bone tissue formation induced by fiber-reinforced scaffolds cultured with osteoblasts and endothelial cells.
abstract: The repair of the damaged bone tissue caused by damage or bone disease was still a problem. Current strategies including the use of autografts and allografts have the disadvantages, namely, diseases transmission, tissue availability and donor morbidity. Bone tissue engineering has been developed and regarded as a new way of regenerating bone tissues to repair or substitute damaged or diseased ones. The main limitation in engineering in vitro tissues is the lack of a sufficient blood vessel system, the vascularization. In this paper, a new-typed hydroxyapatite/collagen composite scaffold which was reinforced by chitosan fibers and cultured with osteoblasts and endothelial cells was fabricated. General observation, histological observation, detection of the degree of vascularization, and X-ray examination had been done to learn the effect of vascularized bone repair materials on the regeneration of bone. The results show that new vessel and bone formed using implant cultured with osteoblasts and endothelial cells. Nanofiber-reinforced scaffold cultured with osteoblasts and endothelial cells can induce vascularized bone tissue formation.
authors.affiliation: Department of Orthopaedics, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing 211100, China.
authors.forename: Xinhui
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, The First Hospital of Hebei Medical University, Shijiazhuang 050031, China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing 211100, China.
authors.forename: Chuanyong
authors.initials: C
authors.lastname: Hou
authors.affiliation: Department of Orthopaedics, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing 211100, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing 211100, China.
authors.forename: Yelin
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Orthopaedics, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing 211100, China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Guan
authors.affiliation: Department of Orthopaedics, The First Hospital of Hebei Medical University, Shijiazhuang 050031, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Dong
authors.affiliation: Department of Orthopaedics, The First Hospital of Hebei Medical University, Shijiazhuang 050031, China.
authors.forename: Hongyang
authors.initials: H
authors.lastname: Gao
authors.affiliation: Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Qingling
authors.initials: Q
authors.lastname: Feng
mesh_terms: D001672:Biocompatible Materials / Q000627:therapeutic use; D001808:Blood Vessels / Q000254:growth & development; D001861:Bone Regeneration; D001842:Bone and Bones / Q000166:cytology; D002478:Cells, Cultured; D048271:Chitosan / Q000627:therapeutic use; D042783:Endothelial Cells / Q000166:cytology; D006801:Humans; D057139:Nanofibers / Q000627:therapeutic use*; D018919:Neovascularization, Physiologic / Q000235:genetics; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D048271:Chitosan