title: [Antimicrobial resistance and virulence characteristics of Vibrio parahaemolyticus].
abstract: OBJECTIVE
  To explore drug resistance and virulence characteristics of clinically isolated Vibrio parahaemolyticus (VP).
  
  
  METHODS
  The patient records were reviewed. The laboratory information system was employed to count the quantity of bacterial diarrhea pathogens at our intestinal clinic from 2009 to 2012. The drug resistant phenotype was tested by the method of disk diffusion. Polymerase chain reaction (PCR) was used to detect thermo-stable direct hemolysin (TDH) gene (tdh), TDH-related hemolysin gene (trh) and type III secretion system 2 (T3SS2α, T3SS2β).
  
  
  RESULTS
  The VP infection rate was 37.90% (274/723). Susceptibility test showed that the resistance rates of ampicillin, cefalotin, cefuroxime and amikacin were 100% (216/216), 44.91% (97/216), 24.07% (52/216), 9.26% (20/216). The other 11 kinds of antibiotics were sensitive or intermediate. 96.76% (209/216) VP harbored tdh gene, 1.39% (3/216) trh gene and 100% T3SS2 gene, with 213 strains T3SS2α positive, 2 strains T3SS2β positive and 1 strain both T3SS2α and T3SS2β positive.
  
  
  CONCLUSIONS
  The resistant rate of VP to ampicillin is the highest and VP has different levels of resistance against other antibiotics. Most clinical isolates of VP harbor tdh gene and T3SS2 plays an important role in the pathogenicity of VP.
authors.affiliation: Department of Clinical Laboratory Medicine, Affiliated Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
authors.forename: Yu-feng
authors.initials: YF
authors.lastname: Sun
authors.affiliation: Department of Clinical Laboratory Medicine, Affiliated Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
authors.forename: Yan-yan
authors.initials: YY
authors.lastname: Fan
authors.affiliation: Department of Clinical Laboratory Medicine, Affiliated Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
authors.forename: Yan-fei
authors.initials: YF
authors.lastname: Huang
authors.affiliation: Department of Clinical Laboratory Medicine, Affiliated Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory Medicine, Affiliated Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China. Email: luxinxin@trhos.com.
authors.forename: Xin-xin
authors.initials: XX
authors.lastname: Lu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D014736:Vibrio parahaemolyticus / Q000187:drug effects* / Q000235:genetics / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D000900:Anti-Bacterial Agents