title: Calcium alginate particles for the combined delivery of platelet lysate and vancomycin hydrochloride in chronic skin ulcers.
abstract: The aim of the present work was the development of a powder formulation for the combined delivery of platelet lysate and of a model antibiotic drug, vancomycin hydrochloride (VCM), in chronic skin ulcers. Calcium alginate particles were prepared by freeze-drying beads obtained by ionic gelation method. The experimental conditions adopted permitted the complete loading of VCM and of PDGF AB, the growth factor chosen as representative of those contained in PL. Such particles where able to absorb PBS (mimicking wound exudate), to form a gel and to modulate the release of VCM and of PDGF AB. They are characterized by enhancement properties of human fibroblast proliferation due to PL presence. In particular, PL, when loaded in alginate particles, was able not only to increase the number of viable cells, but also the number of cells in proliferative phase. Such properties were comparable to those of fresh PL indicating the capability of calcium alginate particles to load PL bioactive substances without altering their activity. The formulation developed is characterized by an easier and a less painful administration with respect to traditional gauzes and semisolid preparations and permits the loading in the same dosage form of active substances of different nature avoiding eventual incompatibility problems.
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia, Italy.
authors.forename: Michela
authors.initials: M
authors.lastname: Mori
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia, Italy. Electronic address: silvia.rossi@unipv.it.
authors.forename: Silvia
authors.initials: S
authors.lastname: Rossi
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia, Italy.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Bonferoni
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia, Italy.
authors.forename: Franca
authors.initials: F
authors.lastname: Ferrari
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Sandri
authors.affiliation: Department of Public Health, Experimental and Forensic Medicine, Histology and Embryology Unit, University of Pavia, Pavia, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Riva
authors.affiliation: Immunohaematology and Transfusion Service and Cell Therapy Unit of Fondazione IRCCS, S. Matteo, Pavia, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Del Fante
authors.affiliation: Immunohaematology and Transfusion Service and Cell Therapy Unit of Fondazione IRCCS, S. Matteo, Pavia, Italy.
authors.forename: Cesare
authors.initials: C
authors.lastname: Perotti
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Caramella
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology; D001792:Blood Platelets / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D002626:Chemistry, Pharmaceutical / Q000379:methods; D005612:Freeze Drying; D005782:Gels; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D012883:Skin Ulcer / Q000188:drug therapy* / Q000473:pathology; D014640:Vancomycin / Q000008:administration & dosage / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000464:Alginates; D000900:Anti-Bacterial Agents; D005782:Gels; D006603:Hexuronic Acids; D010982:Platelet-Derived Growth Factor; C095477:platelet-derived growth factor AB; D014640:Vancomycin; D020723:Glucuronic Acid
keywords: Freeze-dried beads; Platelet lysate; Skin ulcers; Vancomycin hydrochloride; Wound healing