title: In vitro models of the metastatic cascade: from local invasion to extravasation.
abstract: A crucial event in the metastatic cascade is the extravasation of circulating cancer cells from blood capillaries to the surrounding tissues. The past 5 years have been characterized by a significant evolution in the development of in vitro extravasation models, which moved from traditional transmigration chambers to more sophisticated microfluidic devices, enabling the study of complex cell-cell and cell-matrix interactions in multicellular, controlled environments. These advanced assays could be applied to screen easily and rapidly a broad spectrum of molecules inhibiting cancer cell endothelial adhesion and extravasation, thus contributing to the design of more focused in vivo tests.
authors.affiliation: Department of Electronics, Information and Bioengineering, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy; Cell and Tissue Engineering Lab, IRCCS Istituto Ortopedico Galeazzi, Via R. Galeazzi 4, 20161 Milano, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Bersini
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
authors.forename: J S
authors.initials: JS
authors.lastname: Jeon
authors.affiliation: Cell and Tissue Engineering Lab, IRCCS Istituto Ortopedico Galeazzi, Via R. Galeazzi 4, 20161 Milano, Italy. Electronic address: matteo.moretti@grupposandonato.it.
authors.forename: Matteo
authors.initials: M
authors.lastname: Moretti
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA. Electronic address: rdkamm@mit.edu.
authors.forename: R D
authors.initials: RD
authors.lastname: Kamm
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D009361:Neoplasm Invasiveness / Q000473:pathology*; D009369:Neoplasms / Q000097:blood / Q000175:diagnosis; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D015398:Signal Transduction / Q000502:physiology