title: Simultaneous Determination of Volatile Constituents from Acorus tatarinowii Schott in Rat Plasma by Gas Chromatography-Mass Spectrometry with Selective Ion Monitoring and Application in Pharmacokinetic Study.
abstract: A sensitive and specific gas chromatographic-mass spectrometry with selected ion monitoring (GC-MS/SIM) method has been developed for simultaneous identification and quantification of α -asarone, β-asarone, and methyl eugenol of Acorus tatarinowii Schott in rat plasma. Chromatographic separation was performed on a Restek Rxi-5MS capillary column (30 m × 0.32 mm × 0.25 μm), using 1-naphthol as internal standard (IS). MS detection of these compounds and IS was performed at m/z 178, 208, 208, and 144. Intra- and interday precisions of all compounds of interest were less than 10%. The recoveries are situated in the range of 92.4-105.2%. Pharmacokinetics of methyl eugenol confirmed to be one-compartment open model, α -asarone and β -asarone was two-compartment open model, respectively. The method will probably be an alternative to simultaneous determination and pharmacokinetic study of volatile ingredients in Acorus tatarinowii Schott.
authors.affiliation: College of Life Sciences, Northwest University, P.O. Box 195, No. 229 Taibai North Road, Xi'an 710069, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Meng
authors.affiliation: College of Life Sciences, Northwest University, P.O. Box 195, No. 229 Taibai North Road, Xi'an 710069, China.
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Life Sciences, Northwest University, P.O. Box 195, No. 229 Taibai North Road, Xi'an 710069, China.
authors.forename: Shixiang
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Northwest University, P.O. Box 195, No. 229 Taibai North Road, Xi'an 710069, China.
authors.forename: Pu
authors.initials: P
authors.lastname: Jia
authors.affiliation: College of Life Sciences, Northwest University, P.O. Box 195, No. 229 Taibai North Road, Xi'an 710069, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Bai
authors.affiliation: College of Life Sciences, Northwest University, P.O. Box 195, No. 229 Taibai North Road, Xi'an 710069, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Liao
authors.affiliation: College of Life Sciences, Northwest University, P.O. Box 195, No. 229 Taibai North Road, Xi'an 710069, China ; Key Lab for New Drugs Research of TCM in Shenzhen, Shenzhen 518057, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zheng