title: Temporal patterns of abundance of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) and mitochondrial DNA analysis of Ae. albopictus in the Central African Republic.
abstract: The invasive Asian tiger mosquito Aedes albopictus (Diptera: Culicidae) was first reported in central Africa in 2000, in Cameroon, with the indigenous mosquito species Ae. aegypti (Diptera: Culicidae). Today, this invasive species is present in almost all countries of the region, including the Central African Republic (CAR), where it was first recorded in 2009. As invasive species of mosquitoes can affect the distribution of native species, resulting in new patterns of vectors and concomitant risk for disease, we undertook a comparative study early and late in the wet season in the capital and the main cities of CAR to document infestation and the ecological preferences of the two species. In addition, we determined the probable geographical origin of invasive populations of Ae. albopictus with two mitochondrial DNA genes, COI and ND5. Analysis revealed that Ae. aegypti was more abundant earlier in the wet season and Ae. albopictus in the late wet season. Used tyres were the most heavily colonized productive larval habitats for both species in both seasons. The invasive species Ae. albopictus predominated over the resident species at all sites in which the two species were sympatric. Mitochondrial DNA analysis revealed broad low genetic diversity, confirming recent introduction of Ae. albopictus in CAR. Phylogeographical analysis based on COI polymorphism indicated that the Ae. albopictus haplotype in the CAR population segregated into two lineages, suggesting multiple sources of Ae. albopictus. These data may have important implications for vector control strategies in central Africa.
authors.affiliation: Institut Pasteur de Bangui, Bangui, Central African Republic.
authors.forename: Basile
authors.initials: B
authors.lastname: Kamgang
authors.affiliation: Institut Pasteur de Bangui, Bangui, Central African Republic.
authors.forename: Carine
authors.initials: C
authors.lastname: Ngoagouni
authors.affiliation: Institut Pasteur de Bangui, Bangui, Central African Republic.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Manirakiza
authors.affiliation: Institut Pasteur de Bangui, Bangui, Central African Republic.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Nakouné
authors.affiliation: Centre International de Recherches Médicales de Franceville, Franceville, Gabon ; Laboratoire des Maladies Infectieuses et Vecteurs: Écologie, Génétique, Évolution et Contrôle, UMR 224-5290, CNRS-IRD-UM1-UM2, IRD Montpellier, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Paupy
authors.affiliation: Institut Pasteur de Bangui, Bangui, Central African Republic.
authors.forename: Mirdad
authors.initials: M
authors.lastname: Kazanji
mesh_terms: D000330:Aedes / Q000145:classification* / Q000235:genetics / Q000254:growth & development*; D000818:Animals; D002488:Central African Republic; D002947:Cities; D016000:Cluster Analysis; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D017753:Ecosystem; D003576:Electron Transport Complex IV / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D058974:Phylogeography; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV