title: Identification of the novel lincosamide resistance gene lnu(E) truncated by ISEnfa5-cfr-ISEnfa5 insertion in Streptococcus suis: de novo synthesis and confirmation of functional activity in Staphylococcus aureus.
abstract: The novel lincosamide resistance gene lnu(E), truncated by insertion of an ISEnfa5-cfr-ISEnfa5 segment, was identified in Streptococcus suis. The gene lnu(E) encodes a 173-amino-acid protein with ≤69.4% identity to other lincosamide nucleotidyltransferases. The lnu(E) gene and its promoter region were de novo synthesized, and Staphylococcus aureus RN4220 carrying a shuttle vector with the cloned lnu(E) gene showed a 16-fold increase in the lincomycin MIC. Mass spectrometry experiments demonstrated that Lnu(E) catalyzed the nucleotidylation of lincomycin.
authors.affiliation: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhao
authors.forename: Sarah
authors.initials: S
authors.lastname: Wendlandt
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Congming
authors.initials: C
authors.lastname: Wu
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Shen
authors.forename: Stefan
authors.initials: S
authors.lastname: Schwarz
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D003001:Cloning, Molecular; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D054643:INDEL Mutation / Q000235:genetics; D055231:Lincosamides / Q000494:pharmacology*; D008969:Molecular Sequence Data; D011815:R Factors / Q000138:chemical synthesis / Q000235:genetics*; D016415:Sequence Alignment; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*; D017011:Streptococcus suis / Q000187:drug effects / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D055231:Lincosamides