title: Identification of a xanthine oxidase-inhibitory component from Sophora flavescens using NMR-based metabolomics.
abstract: We demonstrate that NMR-based metabolomics studies can be used to identify xanthine oxidase-inhibitory compounds in the diethyl ether soluble fraction prepared from a methanolic extract of Sophora flavescens. Loading plot analysis, accompanied by direct comparison of 1H NMR spectraexhibiting characteristic signals, identified compounds exhibiting inhibitory activity. NMR analysis indicated that these characteristic signals were attributed to flavanones such as sophoraflavanone G and kurarinone. Sophoraflavanone G showed inhibitory activity towards xanthine oxidase in an in vitro assay.
authors.affiliation: Department of Pharmacognosy and Natural Medicines, Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Suzuki
authors.affiliation: Department of Pharmacognosy and Natural Medicines, Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Hasuike
authors.affiliation: Department of Pharmacognosy and Natural Medicines, Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
authors.forename: Moeka
authors.initials: M
authors.lastname: Hirabayashi
authors.affiliation: Medicinal Plant Garden, School of Pharmaceutical Sciences, Kitasato University, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Fukuda
authors.affiliation: Department of Natural Medicine and Phytochemistry, Meifi Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Okada
authors.affiliation: Department of Pharmacognosy and Natural Medicines, Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Shirataki
mesh_terms: D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D025341:Principal Component Analysis; D029909:Sophora / Q000737:chemistry*; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors*
chemical_list: D014969:Xanthine Oxidase