title: On-chip electrophoresis in supported lipid bilayer membranes achieved using low potentials.
abstract: A micro supported lipid bilayer (SLB) electrophoresis method was developed, which functions at low potentials and appreciable operating times. To this end, (hydroxymethyl)-ferrocene (FcCH2OH) was employed to provide an electrochemical reaction at the anode and cathode at low applied potential to avoid electrolysis of water. The addition of FcCH2OH did not alter the SLB characteristics or affect biomolecule function, and pH and temperature variations and bubble formation were eliminated. Applying potentials of 0.25-1.2 V during flow gave homogeneous electrical fields and a fast, reversible, and strong build-up of a charged dye-modified lipid in the direction of the oppositely charged electrode. Moreover, streptavidin mobility could be modulated. This method paves the way for further development of analytical devices.
authors.affiliation: Molecular nanoFabrication group and ‡BIOS lab-on-a-chip group, MESA+ Institute for Nanotechnology, University of Twente , P.O. Box 217, 7500 AE Enschede, The Netherlands.
authors.forename: Jasper
authors.initials: J
authors.lastname: van Weerd
authors.forename: Sven O
authors.initials: SO
authors.lastname: Krabbenborg
authors.forename: Jan
authors.initials: J
authors.lastname: Eijkel
authors.forename: Marcel
authors.initials: M
authors.lastname: Karperien
authors.forename: Jurriaan
authors.initials: J
authors.lastname: Huskens
authors.forename: Pascal
authors.initials: P
authors.lastname: Jonkheijm
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000295:instrumentation; D004572:Electrolysis; D004586:Electrophoresis / Q000295:instrumentation*; D005296:Ferrous Compounds / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D000075163:Metallocenes; D008856:Microscopy, Fluorescence; D014867:Water / Q000737:chemistry
chemical_list: D005296:Ferrous Compounds; D008051:Lipid Bilayers; D000075163:Metallocenes; D014867:Water; C004998:ferrocene