title: Blood compatibility and tissue responsiveness on simple and durable methylsiloxane coating.
abstract: This study was conducted to evaluate the blood compatibility and tissue responsiveness of methylsiloxane (MS)-coated inorganic (glass and metal) substrates both in vitro and in vivo. MS was prepared from methyltriethoxysilane (MTES) through hydrolysis of a sol-gel solution at 80 °C. The adhesive strength of the MS coating was evaluated by using a tear-off test, revealing that the MS strongly adhered to the surface of the inorganic substrates. Blood compatibility was evaluated by assessing platelet adhesion and blood plasma coagulation time. The platelet aggregation ratio of the MS-coated glass tube was reduced to 10%, which was much smaller than that of the coating-free glass tubes (99%) and conventional blood-compatible polystyrene (PS) tubes (18%). Coagulation time was measured by active partial thromboplastin time (APTT) test, which showed that MS coating is as inert as PS in activating blood coagulation factor XII. Tissue responsiveness to the bulk MS sample, evaluated by animal test, showed a desirable compatibility comparable to that of the control titanium sample. This study indicated that MS coating is readily available to convert inorganic materials to useful biomaterials that have suitable mechanical strength and are compatible with blood and tissue.
authors.affiliation: Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai 980-8577, Japan; Materials and Structures Laboratory, Tokyo Institute of Technology, Yokohama 226-8503, Japan.
authors.forename: Yasuto
authors.initials: Y
authors.lastname: Hoshikawa
authors.affiliation: Department of Materials Science, Osaka Prefecture University, Osaka 599-8531, Japan; Materials and Structures Laboratory, Tokyo Institute of Technology, Yokohama 226-8503, Japan.
authors.forename: Takamasa
authors.initials: T
authors.lastname: Onoki
authors.affiliation: Materials and Structures Laboratory, Tokyo Institute of Technology, Yokohama 226-8503, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Akao
authors.affiliation: Materials and Structures Laboratory, Tokyo Institute of Technology, Yokohama 226-8503, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Akatsu
authors.affiliation: Department of Chemical Engineering, Nagoya University, Nagoya 464-8603, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tanabe
authors.affiliation: Materials and Structures Laboratory, Tokyo Institute of Technology, Yokohama 226-8503, Japan.
authors.forename: Eiichi
authors.initials: E
authors.lastname: Yasuda
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology*; D005898:Glass / Q000737:chemistry; D010973:Platelet Adhesiveness / Q000187:drug effects; D011137:Polystyrenes / Q000737:chemistry / Q000494:pharmacology; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology; D014914:Whole Blood Coagulation Time / Q000379:methods
chemical_list: D020099:Coated Materials, Biocompatible; D011137:Polystyrenes; D014025:Titanium