title: Modulation of lipogenesis and glucose consumption in HepG2 cells and C2C12 myotubes by sophoricoside.
abstract: Sophoricoside, an isoflavone glycoside isolated from Sophora japonica (Leguminosae), has been widely reported as an immunomodulator. In this study, the effects of sophoricoside on lipogenesis and glucose consumption in HepG2 cells and C2C12 myotubes were investigated. Treatment with sophoricoside at concentrations of 1-10 μM inhibited lipid accumulation in HepG2 cells in a dose-dependent manner. At the same concentration range, no effect on cell viability was observed in the MTT assay. Inhibition of lipogenesis was associated with the downregulation of SREBP-1a, SREBP-1c, SREBP-2 and their downstream target genes (FAS, ACC, HMGR) as revealed by realtime quantitative PCR. The lipid-lowering effect was mediated via the phosphorylation of AMPK. Further investigation of the activities of this isoflavone showed that sophoricoside has the capability to increase glucose uptake by C2C12 myotubes. It also effectively inhibited the activities of α-glucosidase and α-amylase in vitro and remarkably lowered postprandial hyperglycaemia in starch-loaded C57BL6/J mice. These results suggest that sophoricoside is an effective regulator of lipogenesis and glucose consumption and may find utility in the treatment of obesity and type 2 diabetes.
authors.forename: Chongming
authors.initials: C
authors.lastname: Wu
authors.forename: Hong
authors.initials: H
authors.lastname: Luan
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.forename: Xue
authors.initials: X
authors.lastname: Zhang
authors.forename: Ran
authors.initials: R
authors.lastname: Wang
authors.forename: Lifeng
authors.initials: L
authors.lastname: Jin
authors.forename: Peng
authors.initials: P
authors.lastname: Guo
authors.affiliation: Pharmacology and Toxicology Research Center, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China. chenxi@implad.ac.cn.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology*; D002460:Cell Line; D004789:Enzyme Activation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D065089:Glycoside Hydrolase Inhibitors; D056945:Hep G2 Cells; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects; D050155:Lipogenesis / Q000187:drug effects* / Q000235:genetics; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000187:drug effects* / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
chemical_list: D001578:Benzopyrans; D065089:Glycoside Hydrolase Inhibitors; D014157:Transcription Factors; C120642:sophoricoside; D055372:AMP-Activated Protein Kinases; D000516:alpha-Amylases; D005947:Glucose