title: Radioprotective effects of Dragon's blood and its extract against gamma irradiation in mouse bone marrow cells.
abstract: OBJECTIVE
  The radioprotective effects of Dragon's blood (DB) and its extracts (DBE) were investigated using the chromosomal aberrant test, micronucleus and oxidative stress assay for anti-clastogenic and anti-oxidative activity.
  
  
  METHODS
  Adult BALB/C mice were exposed to the whole body irradiation with 4 Gy (60)Co γ-rays. DB and DBE were administered orally once a day from 5 days prior to irradiation treatment to 1 day after irradiation. The mice were sacrificed on 24 h after irradiation. The cells of bone marrow were measured by counting different types of chromosomal aberrations and the frequency of micronuclei. Oxidative stress response was carried out by analysis of serum from blood.
  
  
  RESULTS
  DB and DBE significantly decreased the number of bone marrow cells with chromosome aberrations after irradiation with respect to irradiated alone group. The administration of DB and DBE also significantly reduced the frequencies of micronucleated polychromatic erythrocytes (MPCE) and micronucleated normochromatic erythrocytes (MNCE). In addition, DB and DBE markedly increased the activity of antioxidant enzymes and the level of antioxidant molecular. Malondialdehyde (MDA) and nitric oxide (NO) levels in serum were significantly reduced by DB and DBE treatment.
  
  
  CONCLUSIONS
  Our data suggested that DB and DBE have potential radioprotective properties in mouse bone marrow after (60)Co γ-ray exposure, which support their candidature as a potential radioprotective agent.
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Ran
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Ran
authors.initials: R
authors.lastname: Wang
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Fankai
authors.initials: F
authors.lastname: Lin
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Murtaza
authors.initials: M
authors.lastname: Hasan
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Qiutian
authors.initials: Q
authors.lastname: Jia
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Tang
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xia
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Shuangquan
authors.initials: S
authors.lastname: Shan
authors.affiliation: China Institute of Atomic Energy, Beijing 102413, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Lanzhou Institute of Modern Physics, Lanzhou 730000, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China. Electronic address: deng@bit.edu.cn.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Deng
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China. Electronic address: hqing@bit.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Qing
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D002869:Chromosome Aberrations / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D005720:Gamma Rays / Q000009:adverse effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015162:Micronucleus Tests; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D010936:Plant Extracts / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts; D011837:Radiation-Protective Agents; C525191:dragon's blood
keywords: Bone marrow; Dragon's blood; Irradiation; Oxidative stress; Radioprotective effects