title: Contrasting effects of pyoverdine on the phytoextraction of Cu and Cd in a calcareous soil.
abstract: Enhanced metal phytoextraction by the use of siderophore-producing bacteria (SPB) has received a lot of attention in the past decade. Bacterial siderophores are able to bind a wide range of metals other than iron and thus should enhance their phytoavailability in contaminated matrices. However, the impact of bacterial siderophores in the soil-plant transfer of metals is not yet fully elucidated, as underlined by the opposing results reported in the literature regarding the efficiency of coupling phytoextraction with bioaugmentation by SPB. The present study focuses on one bacterial siderophore, the pyoverdine (Pvd), produced by Pseudomonas aeruginosa. The coordination properties of Pvd towards Cd(II) and Cu(II) were determined and the effect of Pvd supply was assessed on (i) the mobility (CaCl2 extractions), (ii) the phytoavailability (DGT measurements) and (iii) the phytoextraction of Cd and Cu, in a calcareous soil. The stability constant of Pvd-Cu (KL'Cu=10(20.1)) was found much higher than that of Pvd-Cd (KL'Cd=10(8.2)). The major finding was the agreement observed between Pvd coordination properties and Pvd impact on metals phytoextraction. Pyoverdine, supplied at 250 μmol kg(-1) soil, enhanced the mobility, the phytoavailability and the phytoextraction of Cu while the fate of Cd was not affected. All these results were compared to those reported for chelate-assisted phytoextraction. Their relevance in using SPB for phytoremediation is discussed.
authors.affiliation: INRA (Institut National de la Recherche Agronomique), UMR 1220 TCEM (Transfert sol-plante et Cycle des Eléments Minéraux dans les écosystèmes cultivés), CS 20032, 33882 Villenave d'Ornon cedex, France; Université de Haute Alsace, EA 3991 LVBE (Laboratoire Vigne Biotechnologies Environnement), Equipe Dépollution Biologique des Sols, BP 50568, 68008 Colmar cedex, France. Electronic address: jycornu@bordeaux.inra.fr.
authors.forename: J Y
authors.initials: JY
authors.lastname: Cornu
authors.affiliation: CNRS-Université de Strasbourg, UMR 7509 Laboratoire de Chimie Moléculaire, Equipe Chimie Bioorganique et Médicinale, 25 rue Becquerel, 67200 Strasbourg, France.
authors.forename: M
authors.initials: M
authors.lastname: Elhabiri
authors.affiliation: UMR 7242, Université de Strasbourg-CNRS, ESBS, 300 Boulevard Sébastien Brant, F-67412 Illkirch cedex, Strasbourg, France.
authors.forename: C
authors.initials: C
authors.lastname: Ferret
authors.affiliation: UMR 7242, Université de Strasbourg-CNRS, ESBS, 300 Boulevard Sébastien Brant, F-67412 Illkirch cedex, Strasbourg, France.
authors.forename: V A
authors.initials: VA
authors.lastname: Geoffroy
authors.affiliation: Université de Haute Alsace, EA 3991 LVBE (Laboratoire Vigne Biotechnologies Environnement), Equipe Dépollution Biologique des Sols, BP 50568, 68008 Colmar cedex, France.
authors.forename: K
authors.initials: K
authors.lastname: Jezequel
authors.affiliation: Université de Haute Alsace, EA 3991 LVBE (Laboratoire Vigne Biotechnologies Environnement), Equipe Dépollution Biologique des Sols, BP 50568, 68008 Colmar cedex, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Leva
authors.affiliation: Université de Haute Alsace, EA 3991 LVBE (Laboratoire Vigne Biotechnologies Environnement), Equipe Dépollution Biologique des Sols, BP 50568, 68008 Colmar cedex, France.
authors.forename: M
authors.initials: M
authors.lastname: Lollier
authors.affiliation: UMR 7242, Université de Strasbourg-CNRS, ESBS, 300 Boulevard Sébastien Brant, F-67412 Illkirch cedex, Strasbourg, France.
authors.forename: I J
authors.initials: IJ
authors.lastname: Schalk
authors.affiliation: Université de Haute Alsace, EA 3991 LVBE (Laboratoire Vigne Biotechnologies Environnement), Equipe Dépollution Biologique des Sols, BP 50568, 68008 Colmar cedex, France; LUNAM, LPGN UMR 6112 CNRS, Université de Nantes, 2 rue de la Houssinière, BP 92208, 44322 Nantes cedex 3, France.
authors.forename: T
authors.initials: T
authors.lastname: Lebeau
mesh_terms: D001673:Biodegradation, Environmental; D002104:Cadmium / Q000378:metabolism*; D003300:Copper / Q000378:metabolism*; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D017262:Siderophores / Q000737:chemistry* / Q000378:metabolism; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D009842:Oligopeptides; D017262:Siderophores; D012989:Soil Pollutants; D002104:Cadmium; D003300:Copper; C042453:pyoverdin
keywords: Bacterial siderophore; Bioavailability; Complexation; Coordination properties; DGT; Metals