title: Green fluorescent diamidines as diagnostic probes for trypanosomes.
abstract: Light-emitting diode (LED) fluorescence microscopy offers potential benefits in the diagnosis of human African trypanosomiasis and in other aspects of diseases management, such as detection of drug-resistant strains. To advance such approaches, reliable and specific fluorescent markers to stain parasites in human fluids are needed. Here we describe a series of novel green fluorescent diamidines and their suitability as probes with which to stain trypanosomes.
authors.affiliation: Department of Chemistry, Georgia State University, Atlanta, Georgia, USA.
authors.forename: Federica
authors.initials: F
authors.lastname: Giordani
authors.forename: Manoj
authors.initials: M
authors.lastname: Munde
authors.forename: W David
authors.initials: WD
authors.lastname: Wilson
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Ismail
authors.forename: Arvind
authors.initials: A
authors.lastname: Kumar
authors.forename: David W
authors.initials: DW
authors.lastname: Boykin
authors.forename: Michael P
authors.initials: MP
authors.lastname: Barrett
mesh_terms: D005456:Fluorescent Dyes; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D010419:Pentamidine / Q000031:analogs & derivatives / Q000494:pharmacology; D014344:Trypanocidal Agents / Q000494:pharmacology; D014347:Trypanosoma brucei gambiense / Q000187:drug effects / Q000378:metabolism*; D014353:Trypanosomiasis, African / Q000175:diagnosis*
chemical_list: D005456:Fluorescent Dyes; D014344:Trypanocidal Agents; D010419:Pentamidine