title: Immunoregulatory activities of Dendrobium huoshanense polysaccharides in mouse intestine, spleen and liver.
abstract: To evaluate the immunomodulating responses in intestine, spleen and liver, 50-200mg/kg of DHP was orally administrated to mice without or with methotrexate. The proliferation of marrow cells, which was performed with the addition of the supernatant of small intestinal lymphocytes isolated from the mice administrated orally with DHP, showed that the intestinal immune response was significantly enhanced in all DHP-treated groups. For the immune response in spleen, all tested doses of DHP remarkably promoted the proliferation of splenic cells and increased the secretion of interferon-γ (IFN-γ). For the immune responses in liver, DHP not only significantly stimulated the proliferation of hepatic cells and the secretion of IFN-γ at all tested doses of DHP, but also significantly elevated the secretion interleukin-4 (IL-4) at the doses of 100 and 200mg/kg. Moreover, DHP could recover methotrexate-injured small intestinal immune function (100 and 200mg/kg) and promoted cell proliferation and IFN-γ production (200mg/kg) in spleen and liver of methotrexate-treated mice. These results suggested that DHP after oral administration possessed immunomodulating effects both in small intestine immune system and in systemic immune system, which were further proved by the mRNA expression of IFN-γ and IL-4.
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, PR China.
authors.forename: Xue-Qiang
authors.initials: XQ
authors.lastname: Zha
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, PR China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Zhao
authors.affiliation: Department of Chemistry, University of Puerto Rico at Cayey, No. 225 Antonio R. Barcelo Avenue, Cayey, PR 00736-9997, United States.
authors.forename: Vibha
authors.initials: V
authors.lastname: Bansal
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, PR China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Pan
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, PR China.
authors.forename: Zheng-Ming
authors.initials: ZM
authors.lastname: Wang
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, PR China. Electronic address: jianpingluo@hfut.edu.cn.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Luo
mesh_terms: D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D031667:Dendrobium / Q000737:chemistry*; D007155:Immunologic Factors / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D007422:Intestines / Q000187:drug effects* / Q000276:immunology; D008099:Liver / Q000187:drug effects* / Q000276:immunology; D008297:Male; D008727:Methotrexate / Q000494:pharmacology; D051379:Mice; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects* / Q000276:immunology
chemical_list: D016207:Cytokines; D007155:Immunologic Factors; D011134:Polysaccharides; D008727:Methotrexate
keywords: Dendrobium huoshanense; Immune response; Polysaccharides