title: [MCPIP1 mediates MCP-1-induced vascular smooth muscle cell proliferation].
abstract: The aim of the present study is to investigate whether monocyte chemotactic protein-1 (MCP-1)-induced vascular smooth muscle cell (VSMC) proliferation is mediated via monocyte chemotactic protein-1 induced protein-1 (MCPIP1). MCPIP1 expressions in cultured VSMC were detected by real-time PCR and Western blot following MCP-1 incubation. After MCPIP1 was silenced by siRNA, cell number was counted by hemocytometer, VSMC activity was analyzed by CCK-8 kit, percentage of DNA synthesis was detected by EdU kit, percentage of S phase cell numbers were measured by flow cytometry, and c-fos mRNA expression induced by MCP-1 or platelet-derived growth factor (PDGF) was detected by real-time PCR. The results showed MCP-1 increased MCPIP1 mRNA and up-regulated MCPIP1 protein expression in dose- and time-dependent manners. Cell counts, cellular activity, the percentage of DNA synthesis, and the percentage of S phase cell numbers were remarkably decreased in MCPIP1 siRNA group, compared with those in MCP-1 group. The enhancing effect of MCP-1 or PDGF on c-fos mRNA expression was inhibited by MCPIP1 siRNA. These results suggest that MCP-1-induced VSMC proliferation is mediated via MCPIP1, and the underlying mechanism may involve c-fos expression up-regulation.
authors.affiliation: Department of Biochemistry, Zunyi Medical College, Zunyi 563003, China. qian_mzh@hotmail.com.
authors.forename: Yi-Jun
authors.initials: YJ
authors.lastname: Yang
authors.forename: Liu-Song
authors.initials: LS
authors.lastname: Wu
authors.forename: Bo
authors.initials: B
authors.lastname: Shu
authors.forename: Min-Zhang
authors.initials: MZ
authors.lastname: Qian
mesh_terms: D049109:Cell Proliferation; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000494:pharmacology*; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D060888:Real-Time Polymerase Chain Reaction; D012260:Ribonucleases / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D018932:Chemokine CCL2; D010982:Platelet-Derived Growth Factor; D014157:Transcription Factors; D012260:Ribonucleases; C510646:ZC3H12A protein, human