title: Tandem mass spectrometry of low solubility polyamides.
abstract: The structural characterization of polyamides (PA) was achieved by tandem mass spectrometry (MS/MS) with a laser induced dissociation (LID) strategy. Because of interferences for precursor ions selection, two chemical modifications of the polymer end groups were proposed as derivatization strategies. The first approach, based on the addition of a trifluoroacetic acid (TFA) molecule, yields principally to complementary bn and yn product ions. This fragmentation types, analogous to those obtained with peptides or other PA, give only poor characterization of polymer end-groups [1]. A second approach, based on the addition of a basic diethylamine (DEA), permits to fix the charge and favorably direct the fragmentation. In this case, bn ions were not observed. The full characterization of ω end group structure was obtained, in addition to the expected yn and consecutive fragment ions.
authors.affiliation: Normandie Université, COBRA, UMR 6014 et FR 3038, Université de Rouen, INSA de Rouen, CNRS, 1 rue Tesnière, 76821 Mont Saint Aignan Cedex, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Barrère
authors.affiliation: Normandie Université, COBRA, UMR 6014 et FR 3038, Université de Rouen, INSA de Rouen, CNRS, 1 rue Tesnière, 76821 Mont Saint Aignan Cedex, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Hubert-Roux
authors.affiliation: Normandie Université, COBRA, UMR 6014 et FR 3038, Université de Rouen, INSA de Rouen, CNRS, 1 rue Tesnière, 76821 Mont Saint Aignan Cedex, France.
authors.forename: Carlos
authors.initials: C
authors.lastname: Afonso
authors.affiliation: Normandie Université, INSA de Rouen, CNRS UMR 6270 PBS & FR 3038, Avenue de l'Université, 76801 St Etienne du Rouvray, France.
authors.forename: Majed
authors.initials: M
authors.lastname: Rejaibi
authors.affiliation: Normandie Université, INSA de Rouen, CNRS UMR 6270 PBS & FR 3038, Avenue de l'Université, 76801 St Etienne du Rouvray, France.
authors.forename: Nasreddine
authors.initials: N
authors.lastname: Kebir
authors.affiliation: Normandie Université, INSA de Rouen, CNRS UMR 6270 PBS & FR 3038, Avenue de l'Université, 76801 St Etienne du Rouvray, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Désilles
authors.affiliation: Normandie Université, INSA de Rouen, CNRS UMR 6270 PBS & FR 3038, Avenue de l'Université, 76801 St Etienne du Rouvray, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Lecamp
authors.affiliation: Normandie Université, INSA de Rouen, CNRS UMR 6270 PBS & FR 3038, Avenue de l'Université, 76801 St Etienne du Rouvray, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Burel
authors.affiliation: Normandie Université, COBRA, UMR 6014 et FR 3038, Université de Rouen, INSA de Rouen, CNRS, 1 rue Tesnière, 76821 Mont Saint Aignan Cedex, France. Electronic address: Corinne.Loutelier@univ-rouen.fr.
authors.forename: Corinne
authors.initials: C
authors.lastname: Loutelier-Bourhis
keywords: Derivatization; Fragmentation rules; MALDI-MS/MS; Polyamides