title: Comparison of collagen membrane and bone substitute as a carrier for rhBMP-2 in lateral onlay graft.
abstract: OBJECTIVE
  To evaluate the bone regenerative effect of bioresorbable collagen membrane (CM) as a carrier for recombinant human bone morphogenetic protein-2 (rhBMP-2) when performing lateral onlay grafts using bovine hydroxyapatite incorporated with collagen matrix (BHC) in combination with CM in dogs.
  
  
  METHODS
  A guided bone regeneration (GBR) was performed at the buccal aspect of edentulous maxillary alveolar ridges in dogs (n = 5): (1) BHC group, in which rhBMP-2-loaded BHC was covered by a CM, and (2) CM group, in which BHC was covered by an rhBMP-2-loaded CM. A histologic and histometric analysis was performed after 8 weeks of healing.
  
  
  RESULTS
  Both the BHC and CM groups exhibited substantial newly formed bone (NB). More NB was found in the CM group than in the BHC group without statistical significance. Most of the NB was in direct contact with the residual bone substitute in the BHC group, whereas the projections and islands of NB were observed in the spaces between the residual bone substitute clusters in the CM group. The bone-to-residual bone substitute contact ratio was significantly lower in the CM group than in the BHC group (P = 0.043).
  
  
  CONCLUSIONS
  Within the limitations of this study, it can be concluded that rhBMP-2-loaded CM performed lateral onlay grafts as effectively as rhBMP-2-loaded BHC while showing less bone-residual bone substitute contact ratio in dogs. The loading of CMs with rhBMP-2 might therefore be a recommendable treatment option for facilitating lateral onlay graft combined with rhBMP-2.
authors.affiliation: Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seoul, Korea.
authors.forename: Yun-Young
authors.initials: YY
authors.lastname: Chang
authors.forename: Jung-Seok
authors.initials: JS
authors.lastname: Lee
authors.forename: Min-Soo
authors.initials: MS
authors.lastname: Kim
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Choi
authors.forename: Jung-Kiu
authors.initials: JK
authors.lastname: Chai
authors.forename: Ui-Won
authors.initials: UW
authors.lastname: Jung
mesh_terms: D020341:Absorbable Implants; D000539:Alveolar Process / Q000601:surgery*; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000494:pharmacology*; D003094:Collagen / Q000494:pharmacology*; D004285:Dogs; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D008437:Maxilla / Q000601:surgery; D008567:Membranes, Artificial; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000008:administration & dosage / Q000494:pharmacology*; D014945:Wound Healing / Q000502:physiology
chemical_list: D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D008567:Membranes, Artificial; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D003094:Collagen
keywords: Bio-OSS; bone morphogenetic protein 2; bone regeneration; collagen; histology