title: Paper based colorimetric biosensing platform utilizing cross-linked siloxane as probe.
abstract: Paper based colorimetric biosensing platform utilizing cross-linked siloxane 3-aminopropyltriethoxysilane (APTMS) as probe was developed for the detection of a broad range of targets including H2O2, glucose and protein biomarker. APTMS was extensively used for the modification of filter papers to develop paper based analytical devices. We discovered when APTMS was cross-linked with glutaraldehyde (GA), the resulting complex (APTMS-GA) displays brick-red color, and a visual color change was observed when the complex reacted with H2O2. By integrating the APTMS-GA complex with filter paper, the modified paper enables quantitative detection of H2O2 through the monitoring of the color intensity change of the paper via software Image J. Then, with the immobilization of glucose oxidase (GOx) onto the modified paper, glucose can be detected through the detection of enzymatically generated H2O2. For protein biomarker prostate specific antigen (PSA) assay, we immobilized capture, not captured anti-PSA antibody (Ab1) onto the paper surface and using GOx modified gold nanorod (GNR) as detection anti-PSA antibody (Ab2) label. The detection of PSA was also achieved via the liberated H2O2 when the GOx label reacted with glucose. The results demonstrated the possibility of this paper based sensor for the detection of different analytes with wide linear range. The low cost and simplicity of this paper based sensor could be developed for "point-of-care" analysis and find wide application in different areas.
authors.affiliation: Key Laboratory of Resources Chemistry of Nonferrous Metals, Ministry of Education, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Resources Chemistry of Nonferrous Metals, Ministry of Education, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. Electronic address: yangminghui@csu.edu.cn.
authors.forename: Minghui
authors.initials: M
authors.lastname: Yang
authors.affiliation: Key Laboratory of Resources Chemistry of Nonferrous Metals, Ministry of Education, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China; Department of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, California 90032, USA.
authors.forename: Feimeng
authors.initials: F
authors.lastname: Zhou
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003124:Colorimetry / Q000295:instrumentation*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D010209:Paper; D011437:Propylamines; D011934:Reagent Strips; D012821:Silanes / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; D011437:Propylamines; D011934:Reagent Strips; D012821:Silanes; C477625:amino-propyl-triethoxysilane
keywords: 3-aminopropyltriethoxysilane; Biosensing; Colorimetric; Paper; Point-of-care