title: Effects of the acid-tolerant engineered bacterial strain Megasphaera elsdenii H6F32 on ruminal pH and the lactic acid concentration of simulated rumen acidosis in vitro.
abstract: The aim of this study was to examine the effects of the acid-tolerant engineered bacterial strain Megasphaera elsdenii H6F32 (M. elsdenii H6F32) on ruminal pH and the lactic acid concentrations in simulated rumen acidosis conditions in vitro. A mixed culture of ruminal bacteria, buffer, and primarily degradable substrates was inoculated with equal numbers of M. elsdenii H6 or M. elsdenii H6F32. The pH and lactic acid concentrations in the mixed culture were determined at 0, 2, 4, 6, 8, 10, 12, 14, 16, and 18 h of incubation. Acid-tolerant M. elsdenii H6F32 reduced the accumulation of lactic acid and increased the pH value. These results indicate that acid-tolerant M. elsdenii H6F32 could be a potential candidate for preventing rumen acidosis.
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China; College of Veterinary Medicine, Jilin University, Jilin 130062, China.
authors.forename: M
authors.initials: M
authors.lastname: Long
authors.affiliation: Wellhope Agri-rech Co., Ltd., Shenyang 110164, China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Feng
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: P
authors.initials: P
authors.lastname: Li
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: R X
authors.initials: RX
authors.lastname: He
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: L H
authors.initials: LH
authors.lastname: Yu
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: J B
authors.initials: JB
authors.lastname: He
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: W Y
authors.initials: WY
authors.lastname: Jing
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Y M
authors.initials: YM
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin 130062, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Jilin University, Jilin 130062, China. Electronic address: guowenliu73@126.com.
authors.forename: G W
authors.initials: GW
authors.lastname: Liu
mesh_terms: D000138:Acidosis / Q000378:metabolism / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D002417:Cattle / Q000378:metabolism* / Q000382:microbiology; D005260:Female; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D019344:Lactic Acid / Q000378:metabolism; D045851:Megasphaera / Q000378:metabolism*; D012417:Rumen / Q000378:metabolism* / Q000382:microbiology
chemical_list: D019344:Lactic Acid
keywords: Acid-tolerant; Megasphaera elsdenii; Rumen acidosis