title: [Preliminary study of breast cancer magnetic resonance targeting diagnosis basing on magnetic nanoparticles in vitro].
abstract: OBJECTIVE
  To develop a magnetic nanoparticles based magnetic resonance (MR) probe targeting CD40 mutant in the imaging of breast cancer cells in vitro.
  
  
  METHODS
  For preparing an immunologically competent probe, monoclonal antibody was conjugated with ultrasmall superparamagnetic iron oxide (USPIO) particles basing on chemical cross-linking method.Its bioactivity was analyzed with flow cytometry, confocal microscopy and Prussian blue staining. The probe's cell MR imaging in vitro was conducted on breast cancer cells (M231) high expressing CD40 mutant. The signal data from different groups were collected and analyzed with one-way variance and least significant difference-t test.
  
  
  RESULTS
  The molecular probe carrying nanoparticles and CD40 mutant antibody was constructed and separated successfully. The probe had similar magnetic property compared with original USPIO particles.It could recognize CD40 mutant on breast cancer cells (M231) with high specificity. MR cell imaging in vitro shows that T2 and T2(*) obviously shortened after probe binding with M231 cells and T2 weighted imaging become darker than control groups, the time of T2 is 5H6-USPIO (51.66 ± 5.31) , 5C11-USPIO (92.89 ± 4.72), USPIO (64.56 ± 3.85) ms. The T2 and T2(*) relaxation time of experiment group was shorter than control groups with statistical significance (P < 0.01) .
  
  
  CONCLUSIONS
  MR molecular probe targeting CD40 mutant may bind with breast cancer cells (M231) to provide further in vivo animal MR imaging. And CD40 mutant is expected to provide a new target for MR molecular imaging of breast cancer.
authors.affiliation: Department of Radiology, Subei People's Hospital of Jiangsu Province, Yangzhou 225001, China.
authors.forename: Xian-fu
authors.initials: XF
authors.lastname: Luo
authors.affiliation: Department of Radiology, Subei People's Hospital of Jiangsu Province, Yangzhou 225001, China. Email: wujingtaodoctor@126.com.
authors.forename: Jing-tao
authors.initials: JT
authors.lastname: Wu
authors.affiliation: Department of Radiology, Subei People's Hospital of Jiangsu Province, Yangzhou 225001, China.
authors.forename: Qiu-xia
authors.initials: QX
authors.lastname: Qu
authors.affiliation: Department of Radiology, Subei People's Hospital of Jiangsu Province, Yangzhou 225001, China.
authors.forename: Xiao-hua
authors.initials: XH
authors.lastname: Hu
authors.affiliation: Department of Radiology, Subei People's Hospital of Jiangsu Province, Yangzhou 225001, China.
authors.forename: Ming-xiang
authors.initials: MX
authors.lastname: Chen
authors.affiliation: Department of Radiology, Subei People's Hospital of Jiangsu Province, Yangzhou 225001, China.
authors.forename: Wen-xin
authors.initials: WX
authors.lastname: Chen
authors.affiliation: Department of Radiology, Subei People's Hospital of Jiangsu Province, Yangzhou 225001, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Department of Radiology, Subei People's Hospital of Jiangsu Province, Yangzhou 225001, China.
authors.forename: Lun
authors.initials: L
authors.lastname: Jiang
mesh_terms: D001943:Breast Neoplasms / Q000175:diagnosis*; D019013:CD40 Antigens / Q000235:genetics; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D015335:Molecular Probes; D053758:Nanoparticles
chemical_list: D019013:CD40 Antigens; D015335:Molecular Probes