title: [Progress in markerless knockout of chromosome genes in Streptomycetes].
abstract: Streptomycetes are Gram-positive bacteria of Actinomycetales. These organisms can produce many economically important secondary metabolites. With the development of molecular biology, gene sequencing technology and synthetic biology, people gained a better understanding of the Streptomyces family. The means to transform genome on the molecular level is also increasing. By simplifying the Streptomyces genome rationally and efficiently, it will improve the yield and quality of the metabolites as well as reduce the consumption of the substrates. Markerless knockout is an important way to carry out genetic modification. Here we describe novel genome modification techniques developed for Streptomyces in recent years with focus on the markerless knockout technologies.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Gu
authors.forename: Weitao
authors.initials: W
authors.lastname: Geng
authors.forename: Cunjiang
authors.initials: C
authors.lastname: Song
mesh_terms: D002876:Chromosomes, Bacterial / Q000235:genetics*; D055786:Gene Knockout Techniques / Q000379:methods*; D005798:Genes, Bacterial / Q000235:genetics*; D013302:Streptomyces / Q000235:genetics*