title: Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography.
abstract: Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 Å resolution and determine its serial femtosecond crystallography structure to 3.5 Å resolution. Although every microcrystal is exposed to a dose of 33 MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30 Gothenburg, Sweden.
authors.forename: Linda C
authors.initials: LC
authors.lastname: Johansson
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30 Gothenburg, Sweden.
authors.forename: David
authors.initials: D
authors.lastname: Arnlund
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30 Gothenburg, Sweden.
authors.forename: Gergely
authors.initials: G
authors.lastname: Katona
authors.affiliation: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: White
authors.affiliation: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Anton
authors.initials: A
authors.lastname: Barty
authors.affiliation: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: DePonte
authors.affiliation: 1] Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany [2] Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Shoeman
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30 Gothenburg, Sweden.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Wickstrand
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30 Gothenburg, Sweden.
authors.forename: Amit
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Linac Coherent Light Source, LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, 94025 California, USA.
authors.forename: Garth J
authors.initials: GJ
authors.lastname: Williams
authors.affiliation: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Andrew
authors.initials: A
authors.lastname: Aquila
authors.affiliation: PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, 94025 California, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Bogan
authors.affiliation: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Carl
authors.initials: C
authors.lastname: Caleman
authors.affiliation: Department of Chemistry-Ångström Laboratory, Uppsala University, Box 523, 75120 Uppsala, Sweden.
authors.forename: Jan
authors.initials: J
authors.lastname: Davidsson
authors.affiliation: Department of Physics, Arizona State University, Tempe, 85287 Arizona, USA.
authors.forename: R Bruce
authors.initials: RB
authors.lastname: Doak
authors.affiliation: Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, 94550 California, USA.
authors.forename: Matthias
authors.initials: M
authors.lastname: Frank
authors.affiliation: Department of Chemistry and Biochemistry, Arizona State University, Tempe, 85287-1604 Arizona, USA.
authors.forename: Raimund
authors.initials: R
authors.lastname: Fromme
authors.affiliation: 1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [2] Department of Physics, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Galli
authors.affiliation: Department of Chemistry and Biochemistry, Arizona State University, Tempe, 85287-1604 Arizona, USA.
authors.forename: Ingo
authors.initials: I
authors.lastname: Grotjohann
authors.affiliation: Department of Chemistry and Biochemistry, Arizona State University, Tempe, 85287-1604 Arizona, USA.
authors.forename: Mark S
authors.initials: MS
authors.lastname: Hunter
authors.affiliation: 1] Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany [2] Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Kassemeyer
authors.affiliation: Department of Physics, Arizona State University, Tempe, 85287 Arizona, USA.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Kirian
authors.affiliation: Department of Chemistry and Biochemistry, Arizona State University, Tempe, 85287-1604 Arizona, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Kupitz
authors.affiliation: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Mengning
authors.initials: M
authors.lastname: Liang
authors.affiliation: 1] Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany [2] Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Lukas
authors.initials: L
authors.lastname: Lomb
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30 Gothenburg, Sweden.
authors.forename: Erik
authors.initials: E
authors.lastname: Malmerberg
authors.affiliation: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Andrew V
authors.initials: AV
authors.lastname: Martin
authors.affiliation: Linac Coherent Light Source, LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, 94025 California, USA.
authors.forename: Marc
authors.initials: M
authors.lastname: Messerschmidt
authors.affiliation: 1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [2] Department of Physics, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.
authors.forename: Karol
authors.initials: K
authors.lastname: Nass
authors.affiliation: Joint Laboratory for Structural Biology of Infection and Inflammation, Institute of Biochemistry and Molecular Biology, University of Hamburg and Institute of Biochemistry, University of Lübeck at DESY, 22607 Hamburg, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Redecke
authors.affiliation: Linac Coherent Light Source, LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, 94025 California, USA.
authors.forename: M Marvin
authors.initials: MM
authors.lastname: Seibert
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30 Gothenburg, Sweden.
authors.forename: Jennie
authors.initials: J
authors.lastname: Sjöhamn
authors.affiliation: 1] Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany [2] Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Steinbrener
authors.affiliation: Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Francesco
authors.initials: F
authors.lastname: Stellato
authors.affiliation: Department of Physics, Arizona State University, Tempe, 85287 Arizona, USA.
authors.forename: Dingjie
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30 Gothenburg, Sweden.
authors.forename: Weixaio Y
authors.initials: WY
authors.lastname: Wahlgren
authors.affiliation: Department of Physics, Arizona State University, Tempe, 85287 Arizona, USA.
authors.forename: Uwe
authors.initials: U
authors.lastname: Weierstall
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30 Gothenburg, Sweden.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Westenhoff
authors.affiliation: Department of Physics, Arizona State University, Tempe, 85287 Arizona, USA.
authors.forename: Nadia A
authors.initials: NA
authors.lastname: Zatsepin
authors.affiliation: Linac Coherent Light Source, LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, 94025 California, USA.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Boutet
authors.affiliation: Department of Physics, Arizona State University, Tempe, 85287 Arizona, USA.
authors.forename: John C H
authors.initials: JC
authors.lastname: Spence
authors.affiliation: 1] Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany [2] Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Ilme
authors.initials: I
authors.lastname: Schlichting
authors.affiliation: 1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [2] Department of Physics, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.
authors.forename: Henry N
authors.initials: HN
authors.lastname: Chapman
authors.affiliation: Department of Chemistry and Biochemistry, Arizona State University, Tempe, 85287-1604 Arizona, USA.
authors.forename: Petra
authors.initials: P
authors.lastname: Fromme
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30 Gothenburg, Sweden.
authors.forename: Richard
authors.initials: R
authors.lastname: Neutze
mesh_terms: D018360:Crystallography, X-Ray / Q000379:methods*; D041921:Hyphomicrobiaceae / Q000737:chemistry*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D011487:Protein Conformation
chemical_list: D045322:Photosynthetic Reaction Center Complex Proteins