title: Short and long-term antinutritional effect of the trypsin inhibitor ApTI for biological control of sugarcane borer.
abstract: Plant-derived trypsin inhibitors have been shown to have potent anti-insect effects and are a promising alternative for the biological control of pests. In this work, we tested the anti-insect activity of Adenanthera pavonina trypsin inhibitor (ApTI) against Diatraea saccharalis larvae, a major insect pest in sugarcane. The addition of 0.1% ApTI in short-term assays resulted in 87% and 63% decreased trypsin and chymotrypsin activities respectively. ApTI was not digested after 60h incubation with D. saccharalis midgut proteases. The chronic effects of ApTI on F0 and F1 generations of D. saccharalis were also analyzed. The larvae from the F0 generation showed 55% and 21% decreased larval and pupal viability, respectively. ApTI-fed larvae from the F1 generation showed a decrease of 33% in survival rate and 23% in the average larval weight. Moreover, ApTI treatment reduced trypsin and chymotrypsin activities in F1 larvae. Thus, the anti-insect effects of ApTI on consecutive generations (F0 and F1) of D. saccharalis larvae demonstrate its potential for long-term control of this pest.
authors.affiliation: Departamento de Bioquímica/Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brazil; Departamento de Tecnologia de Alimentos e Saúde Pública, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Desireé S
authors.initials: DS
authors.lastname: da Silva
authors.affiliation: Departamento de Bioquímica/Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brazil; Departamento de Tecnologia de Alimentos e Saúde Pública, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Caio F R
authors.initials: CF
authors.lastname: de Oliveira
authors.affiliation: Departamento de Entomologia e Acarologia, Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz, Piracicaba, SP, Brazil.
authors.forename: José R P
authors.initials: JR
authors.lastname: Parra
authors.affiliation: Departamento de Bioquímica/Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brazil.
authors.forename: Sergio
authors.initials: S
authors.lastname: Marangoni
authors.affiliation: Departamento de Tecnologia de Alimentos e Saúde Pública, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil. Electronic address: bioplant@terra.com.br.
authors.forename: Maria L R
authors.initials: ML
authors.lastname: Macedo
mesh_terms: D000220:Adaptation, Biological; D000824:Animal Nutritional Physiological Phenomena / Q000187:drug effects; D000818:Animals; D007887:Fabaceae / Q000737:chemistry*; D007814:Larva / Q000187:drug effects; D009036:Moths / Q000187:drug effects*; D010572:Pest Control, Biological; D010940:Plant Proteins / Q000494:pharmacology*; D011679:Pupa / Q000187:drug effects; D013997:Time Factors; D014361:Trypsin Inhibitors / Q000494:pharmacology*
chemical_list: D010940:Plant Proteins; D014361:Trypsin Inhibitors
keywords: Adenanthera pavonina; Biological control; Diatraea saccharalis; Plant–insect interaction