title: [Determination of alditols in wine by gas chromatography-mass spectrometry after acetate derivatization].
abstract: The acetate derivatization of alditols for determining alditol level in wine by gas chromatography (GC)-mass spectrometry (MS) has been developed. The wine sample was mixed with pyridine and centrifuged at 5,000 r/min at the temperature of 4 degrees C for 10 min. After filtration with organic phase membrane, the supernatant was derivatized with acetic anhydride, and then dehydrated with anhydrous sodium sulfate. The GC separation was performed on a DB-5MS capillary column. The alditols were determined by MS in selected ion monitoring (SIM) mode and quantified by external standard method. The calibration curves showed good linearities in the range of 0.019 - 1.25 mg/L except for lactitol (0.039 - 2.50 mg/L) with the correlation coefficients greater than 0.99. The limits of quantification (S/N= 10) of erythritol, xylitol, D-mannitol, sorbitol, galactitol and lactitol were 0.17, 0.29, 0.43, 0.46, 0.47 and 2.88 mg/L respectively. The limits of detection (S/N = 3) were 0.05, 0.08, 0.13, 0.14, 0.14 and 1.38 mg/L respectively. The recoveries of alditols spiked in the wine at two levels of 40 mg/L and 80 mg/L were ranged from 80.15% to 108.75% with the relative standard deviations (RSDs) of 2.16% - 6.97%. The sensitivity, accuracy and precision of the method can meet the technical standard. The method can be applied to the rapid determination of alditols in wine.
authors.affiliation: State Key Laboratory of Food Additive and Condiment Testing, Zhenjiang 212008, China. Haoranzzj@sohu.com
authors.forename: Hongbin
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Jiangsu University, Zheniang 212013, China.
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Food Additive and Condiment Testing, Zhenjiang 212008, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Food Additive and Condiment Testing, Zhenjiang 212008, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Wan
authors.affiliation: State Key Laboratory of Food Additive and Condiment Testing, Zhenjiang 212008, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: State Key Laboratory of Food Additive and Condiment Testing, Zhenjiang 212008, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Shen
mesh_terms: D000085:Acetates; D008401:Gas Chromatography-Mass Spectrometry; D013402:Sugar Alcohols / Q000032:analysis*; D014920:Wine / Q000032:analysis*
chemical_list: D000085:Acetates; D013402:Sugar Alcohols