title: Trypsin inhibitor complexes with human and bovine serum albumins: TEM and spectroscopic analysis.
abstract: We report the binding of trypsin inhibitor (TI) with human serum albumin (HSA) and bovine serum albumin (BSA) at physiological conditions, using FTIR, CD, UV-Visible spectroscopic methods and transmission electron microscopy (TEM). Structural analysis showed that trypsin inhibitor binds HSA and BSA via hydrophilic and hydrophobic contacts with overall binding constants of KTI-HSA=1.4 (±0.5)×10(4)M(-1) and KTI-BSA=1.1 (±0.4)×10(6)M(-1). Trypsin inhibitor complexation induces minor reduction of the protein α-helix and a major increase in β-sheet structure. TEM images show that trypsin inhibitor complex formation leads to the protein aggregation and fibrillation.
authors.affiliation: Department of Industrial-Engineering, University of Québec at Trois-Rivières, C.P. 500, TR, Quebec G9A 5H7, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Hebia
authors.affiliation: Department of Chemistry-Physics, University of Québec at Trois-Rivières, C.P. 500, TR, Quebec G9A 5H7, Canada.
authors.forename: L
authors.initials: L
authors.lastname: Bekale
authors.affiliation: Department of Chemistry-Physics, University of Québec at Trois-Rivières, C.P. 500, TR, Quebec G9A 5H7, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Chanphai
authors.affiliation: Department of Chemical-Engineering, University of Québec at Trois-Rivières, C.P. 500, TR, Quebec G9A 5H7, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Agbebavi
authors.affiliation: Department of Chemistry-Physics, University of Québec at Trois-Rivières, C.P. 500, TR, Quebec G9A 5H7, Canada. Electronic address: tajmirri@uqtr.ca.
authors.forename: H A
authors.initials: HA
authors.lastname: Tajmir-Riahi
mesh_terms: D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D046529:Microscopy, Electron, Transmission; D017433:Protein Structure, Secondary; D012709:Serum Albumin / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D014361:Trypsin Inhibitors / Q000737:chemistry*
chemical_list: D012709:Serum Albumin; D014361:Trypsin Inhibitors
keywords: Binding site; Serum albumin; Spectroscopy; TEM images; Trypsin inhibitor