title: Quinoline-based two-photon fluorescent probe for nitric oxide in live cells and tissues.
abstract: A two-photon fluorescent probe (QNO) for nitric oxide is reported. The probe is designed with a photoinduced electron transfer (PeT) mechanism and shows 12-fold fluorescence enhancement toward NO. Adopting a quinoline derivative as the fluorophore, QNO has a large two-photon action cross section value of 52 GM and long-wavelength emission. It also features high selectivity, low cytotoxicity, and pH insensitivity. By utilizing two-photon microscopy (TPM), QNO can detect NO in live cells and live tissues at a depth of 90-180 μm.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University , Wuhan, Hubei 430072, China.
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Dong
authors.forename: Cheol Ho
authors.initials: CH
authors.lastname: Heo
authors.forename: Shiyu
authors.initials: S
authors.lastname: Chen
authors.forename: Hwan Myung
authors.initials: HM
authors.lastname: Kim
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry*; D006624:Hippocampus / Q000737:chemistry* / Q000166:cytology; D008264:Macrophages / Q000737:chemistry*; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D009569:Nitric Oxide / Q000032:analysis* / Q000378:metabolism; D009924:Organ Culture Techniques; D011804:Quinolines / Q000737:chemistry*; D051381:Rats
chemical_list: D005456:Fluorescent Dyes; D011804:Quinolines; D009569:Nitric Oxide; C037219:quinoline