title: Phosphatidic acid interacts with a MYB transcription factor and regulates its nuclear localization and function in Arabidopsis.
abstract: Phosphatidic acid (PA) has emerged as a class of cellular mediators involved in various cellular and physiological processes, but little is known about its mechanism of action. Here we show that PA interacts with werewolf (WER), a R2R3 MYB transcription factor involved in root hair formation. The PA-interacting region is confined to the end of the R2 subdomain. The ablation of the PA binding motif has no effect on WER binding to DNA, but abolishes its nuclear localization and its function in regulating epidermal cell fate. Inhibition of PA production by phospholipase Dζ also suppresses WER's nuclear localization, root hair formation, and elongation. These results suggest a role for PA in promoting protein nuclear localization.
authors.affiliation: Department of Biology, University of Missouri, St. Louis, Missouri 63121.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Yao
authors.forename: Geliang
authors.initials: G
authors.lastname: Wang
authors.forename: Liang
authors.initials: L
authors.lastname: Guo
authors.forename: Xuemin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism* / Q000502:physiology; D001665:Binding Sites; D001692:Biological Transport; D004268:DNA-Binding Proteins / Q000378:metabolism* / Q000502:physiology; D008969:Molecular Sequence Data; D010712:Phosphatidic Acids / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D010712:Phosphatidic Acids; C402111:WER protein, Arabidopsis