title: Highly stable, protein capped gold nanoparticles as effective drug delivery vehicles for amino-glycosidic antibiotics.
abstract: A method for the production of highly stable gold nanoparticles (Au NP) was optimized using sodium borohydride as reducing agent and bovine serum albumin as capping agent. The synthesized nanoparticles were characterized using UV-visible spectroscopy, transmission electron microscopy, X-ray diffraction (XRD) and dynamic light scattering techniques. The formation of gold nanoparticles was confirmed from the appearance of pink colour and an absorption maximum at 532 nm. These protein capped nanoparticles exhibited excellent stability towards pH modification and electrolyte addition. The produced nanoparticles were found to be spherical in shape, nearly monodispersed and with an average particle size of 7.8±1.7 nm. Crystalline nature of the nanoparticles in face centered cubic structure is confirmed from the selected-area electron diffraction and XRD patterns. The nanoparticles were functionalized with various amino-glycosidic antibiotics for utilizing them as drug delivery vehicles. Using Fourier transform infrared spectroscopy, the possible functional groups of antibiotics bound to the nanoparticle surface have been examined. These drug loaded nanoparticle solutions were tested for their antibacterial activity against Gram-negative and Gram-positive bacterial strains, by well diffusion assay. The antibiotic conjugated Au NP exhibited enhanced antibacterial activity, compared to pure antibiotic at the same concentration. Being protein capped and highly stable, these gold nanoparticles can act as effective carriers for drugs and might have considerable applications in the field of infection prevention and therapeutics.
authors.forename: Lori
authors.initials: L
authors.lastname: Rastogi
authors.forename: Aruna Jyothi
authors.initials: AJ
authors.lastname: Kora
authors.forename: Arunachalam
authors.initials: A
authors.lastname: J
mesh_terms: D000617:Aminoglycosides / Q000008:administration & dosage* / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry*; D001894:Borohydrides / Q000737:chemistry; D016503:Drug Delivery Systems; D006046:Gold / Q000737:chemistry*; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests / Q000379:methods; D010316:Particle Size; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D001894:Borohydrides; D012710:Serum Albumin, Bovine; D006046:Gold; C025364:sodium borohydride