title: Polymorphisms of the TLR4 gene and risk of gastric cancer.
abstract: OBJECTIVE
  Toll-like receptor 4 (TLR4) is an important lipo-polysaccharide (LPS) receptor in gastric epithelial cell signaling transduction and plays critical roles in the development and progression of gastric cancer (GC). We investigated the effects of TLR4 gene polymorphisms and gene-environmental interactions on the risk of GC in Northeastern China.
  
  
  METHODS
  We genotyped two single-nucleotide polymorphisms (SNPs) in TLR4 (rs10116253 and rs1927911) in 217 GC patients and 294 cancer-free controls using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Odds ratio (OR) and 95% confidence intervals (CIs) were estimated by unconditional logistic-regression models.
  
  
  RESULTS
  Individuals carrying CC genotype of rs10116253 and TT genotype of rs1927911 had a significantly decreased risk of GC (adjusted OR=0.33, 95% CI 0.18-0.60, P<0.001 and adjusted OR=0.37, 95% CI 0.21-0.67, P=0.001 respectively), compared with TT genotype of rs10116253 and CC genotype of rs1927911. In addition, the SNP effects were additive to the effects of some known environmental factors without any interaction between them in the susceptibility to GC.
  
  
  CONCLUSIONS
  Our data suggested that TLR4 gene polymorphisms may be associated with a decreased risk of GC in Chinese population. And these SNPs and their combined effects with environmental factors may be associated with the risk of GC.
authors.affiliation: Department of Epidemiology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, PR China.
authors.forename: Lina
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Epidemiology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, PR China.
authors.forename: Kexin
authors.initials: K
authors.lastname: Yuan
authors.affiliation: Department of Epidemiology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Epidemiology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, PR China.
authors.forename: Xiyun
authors.initials: X
authors.lastname: Ren
authors.affiliation: Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, The University of Rhode Island, Pharmacy Building, 7 Greenhouse Road, Kingston, RI 02881, USA.
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Epidemiology, College of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, PR China. Electronic address: twj8267@sina.com.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Tian
authors.affiliation: Department of Colorectal Cancer Surgery, The Third Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang Province, PR China. Electronic address: 13945676987@126.com.
authors.forename: Yunhe
authors.initials: Y
authors.lastname: Jia
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China; D005260:Female; D059647:Gene-Environment Interaction; D020022:Genetic Predisposition to Disease; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D013274:Stomach Neoplasms / Q000235:genetics*; D051197:Toll-Like Receptor 4 / Q000235:genetics*
chemical_list: C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4
keywords: CI; DHA; EPA; GC; Gastric cancer; HWE; Hardy–Weinberg equilibrium; LPS; OR; PCR-RFLP; Single-nucleotide polymorphisms; TLR4; TLRs; TNM; Toll-like receptor 4; Toll-like receptors; confidence interval; docosahexaenoic acid; eicosapntemacnioc acid; gastric cancer; lipo-polysaccharide; odds ratio; polymerase chain reaction-restriction fragment length polymorphism; tumor-node-metastasis