title: Antibacterial and wound healing analysis of gelatin/zeolite scaffolds.
abstract: In this article, gelatin/copper activated faujasites (CAF) composite scaffolds were fabricated by lyophilisation technique for promoting partial thickness wound healing. The optimised scaffold with 0.5% (w/w) of CAF, G (0.5%), demonstrated pore size in the range of 10-350 μm. Agar disc diffusion tests verified the antibacterial role of G (0.5%) and further supported that bacterial lysis was due to copper released from the core of CAF embedded in the gelatin matrix. The change in morphology of bacteria as a function of CAF content in gelatin scaffold was studied using SEM analysis. The confocal images revealed the increase in mortality rate of bacteria with increase in concentration of incorporated CAF in gelatin matrix. Proficient oxygen supply to needy cells is a continuing hurdle faced by tissue engineering scaffolds. The dissolved oxygen measurements revealed that CAF embedded in the scaffold were capable of increasing oxygen supply and thereby promote cell proliferation. Also, G (0.5%) exhibited highest cell viability on NIH 3T3 fibroblast cells which was mainly attributed to the highly porous architecture and its ability to enhance oxygen supply to cells. In vivo studies conducted on Sprague Dawley rats revealed the ability of G (0.5%) to promote skin regeneration in 20 days. Thus, the obtained data suggest that G (0.5%) is an ideal candidate for wound healing applications.
authors.affiliation: Université de Bretagne Sud, Laboratoire Ingénierie des Matériaux de Bretagne, BP 92116, 56321 Lorient Cedex, France; Centre for Nanoscience and Nanotechnology and School of Chemical Sciences, Mahatma Gandhi University, Priyadarsini Hills PO, Kottayam 686 560, Kerala, India. Electronic address: neethuninan85@yahoo.co.in.
authors.forename: Neethu
authors.initials: N
authors.lastname: Ninan
authors.affiliation: Department of Biomedical Science and BK21 PLUS Center for Creative Biomedical Scientists, Chonnam National University Medical School, 160 Baekseo-ro, Gwangju 501-746, Republic of Korea.
authors.forename: Muthunarayanan
authors.initials: M
authors.lastname: Muthiah
authors.affiliation: Non-Destructive Biomedical and Pharmaceutical Research Centre, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor, Malaysia.
authors.forename: Nur Aliza
authors.initials: NA
authors.lastname: Bt Yahaya
authors.affiliation: Department of Biomedical Science and BK21 PLUS Center for Creative Biomedical Scientists, Chonnam National University Medical School, 160 Baekseo-ro, Gwangju 501-746, Republic of Korea.
authors.forename: In-Kyu
authors.initials: IK
authors.lastname: Park
authors.affiliation: Université de Bretagne Sud, Laboratoire Ingénierie des Matériaux de Bretagne, BP 92116, 56321 Lorient Cedex, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Elain
authors.affiliation: Non-Destructive Biomedical and Pharmaceutical Research Centre, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor, Malaysia.
authors.forename: Tin Wui
authors.initials: TW
authors.lastname: Wong
authors.affiliation: Centre for Nanoscience and Nanotechnology and School of Chemical Sciences, Mahatma Gandhi University, Priyadarsini Hills PO, Kottayam 686 560, Kerala, India.
authors.forename: Sabu
authors.initials: S
authors.lastname: Thomas
authors.affiliation: Université de Bretagne Sud, Laboratoire Ingénierie des Matériaux de Bretagne, BP 92116, 56321 Lorient Cedex, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Grohens
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003300:Copper / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005780:Gelatin / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008826:Microbial Sensitivity Tests; D041681:NIH 3T3 Cells; D010100:Oxygen / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects*; D017641:Zeolites / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; C539489:faujasite; D017641:Zeolites; D003300:Copper; D005780:Gelatin; D010100:Oxygen
keywords: Animal studies; Anti-bacterial; Copper activated faujasite; Fibroblast; Gelatin; Wound healing