title: An intracellular buildup reaction of active siRNA species from short RNA fragments.
abstract: Here we report a new strategy for the buildup reaction of active siRNA species from short RNA fragments in living cells using a chemical ligation reaction. This strategy could decrease undesired immune responses and provide more latitude for RNAi technology in the design and concentration of introduced RNA compared to traditional siRNA methods.
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, 2-1, Hirosawa, Wako-Shi, Saitama 351-0198, Japan. h-abe@pharm.hokuadai.ac.jp y-ito@riken.jp.
authors.forename: Hideto
authors.initials: H
authors.lastname: Maruyama
authors.forename: Yuko
authors.initials: Y
authors.lastname: Nakashima
authors.forename: Satoshi
authors.initials: S
authors.lastname: Shuto
authors.forename: Akira
authors.initials: A
authors.lastname: Matsuda
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Abe
mesh_terms: D045744:Cell Line, Tumor; D053487:DEAD-box RNA Helicases / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D016899:Interferon-beta / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D043244:Ribonuclease III / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D016899:Interferon-beta; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases