title: Association of FAS and FAS ligand genes polymorphism and risk of systemic lupus erythematosus.
abstract: FAS/FASL pathway plays a critical role in maintaining peripheral immune tolerance; therefore, the apoptosis genes, Fas and Fas ligand (FasL), could be suitable candidate genes in human SLE susceptibility.
  
  
  METHODS
  In this case-control study, 106 SLE patients and 149 sex, age, and ethnicity matched healthy controls were genotyped for the Fas A-670G and FasLC-844T polymorphisms by polymerase chain reaction-restriction fragment length polymorphism method (PCR-RFLP).
  
  
  RESULTS
  The frequency of -670AA genotype was significantly higher in SLE patients than control group and the risk of SLE was 2.1-fold greater in subjects with AA genotype (P=0.03). The frequency of -670A allele was significantly higher in SLE patients than in controls too (58% versus 49%, P=0.03). The -844CC genotype frequency was significantly higher in SLE patients than in healthy controls and the risk of SLE was 2.8-fold greater in these subjects (P=0.01). The C allele frequency was significantly higher in patients than in controls (69% versus 49%, P=0.001). Increased SLE risk was observed in individuals with combined effect of Fas-670AA and FasL-844CC genotypes (P=0.001).
  
  
  CONCLUSIONS
  Fas-670AA and FasL-844CC genotypes were associated with SLE risk, and combined effect of -670AA and -844CC genotypes might increase SLE susceptibility.
authors.affiliation: Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan 9816743175, Iran.
authors.forename: Bita
authors.initials: B
authors.lastname: Moudi
authors.affiliation: Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan 9816743175, Iran ; Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan 9816743175, Iran.
authors.forename: Saeedeh
authors.initials: S
authors.lastname: Salimi
authors.affiliation: Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan 9816743175, Iran ; Department of Pharmacology, School of Medicine, Zahedan University of Medical Sciences, Zahedan 9816743175, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Farajian Mashhadi
authors.affiliation: Department of Internal Medicine, School of Medicine, Zahedan University of Medical Sciences, Zahedan 9816743175, Iran.
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Sandoughi
authors.affiliation: Department of Internal Medicine, School of Medicine, Zahedan University of Medical Sciences, Zahedan 9816743175, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Zakeri
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D053222:Fas Ligand Protein / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D055815:Young Adult; D019014:fas Receptor / Q000235:genetics*
chemical_list: D053222:Fas Ligand Protein; D019014:fas Receptor