title: Stability of soybean oil degumming using immobilized phospholipase A(2).
abstract: The aim of this study was evaluation of stability of immobilized phospholipase A2 (PLA2) for soybean oil degumming. Also, the effect of reaction time on residual phosphorus levels was investigated according to the optimum pH and temperature. The free PLA2 and three immobilized PLA2 demonstrated significant differences in optimum operation conditions. pH, temperature and reaction time increased upon immobilization for three different immobilized PLA2 (PLA2-CA, PLA2-CAC and PLA2-CAG). Immobilized PLA2 showed enhanced thermal stability and retained more than 74% of relative activity after 1 h of incubation at 60°C, while the free PLA2 retained only 33%. The three immobilized PLA2 retained 30% to 60% of initial activities after 7 recycles. In particular, PLA2-CAC has more significant profiles in pH, temperature, reaction time and showed the highest remaining activity, thermal stability, reusability. Therefore, PLA2-CAC is a suitable immobilized enzyme for soybean oil degumming process.
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology.
authors.forename: Dianyu
authors.initials: D
authors.lastname: Yu
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.forename: Lianzhou
authors.initials: L
authors.lastname: Jiang
authors.forename: Elfalleh
authors.initials: E
authors.lastname: Walid
authors.forename: Shenghua
authors.initials: S
authors.lastname: He
authors.forename: Yanming
authors.initials: Y
authors.lastname: He
authors.forename: Zhou
authors.initials: Z
authors.lastname: Xiaoyu
authors.forename: Jianing
authors.initials: J
authors.lastname: Zhang
authors.forename: Lizhi
authors.initials: L
authors.lastname: Hu
mesh_terms: D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism; D005511:Food Handling; D006863:Hydrogen-Ion Concentration; D054467:Phospholipases A2 / Q000378:metabolism*; D010758:Phosphorus / Q000032:analysis; D013024:Soybean Oil / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
chemical_list: D004800:Enzymes, Immobilized; D010758:Phosphorus; D013024:Soybean Oil; D054467:Phospholipases A2