title: Biocrude production by activated sludge microbial cultures using pulp and paper wastewaters as fermentation substrate.
abstract: Municipal wastewater activated sludge contains a mixed microbial community, which can be manipulated to produce biocrude, a lipid feedstock for biodiesel production. In this study, the potential of biocrude production by activated sludge microorganisms grown in three different types of pulp and paper mill wastewaters was investigated. A 20% (v/v) activated sludge was inoculated into pulp and paper wastewater, supplemented with glucose (60 g/L) and nutrients (nitrogen and phosphorus) to obtain a high carbon to nitrogen ratio (70:1). The culture was incubated aerobically for seven days. The results showed that the activated sludge microorganisms were able to grow and accumulate lipids when cultivated in amended wastewaters. Microorganisms growing in anaerobic settling pond effluent water showed the highest lipid accumulation of up to 40.6% cell dry weight (CDW) after five days of cultivation compared with pulp wash wastewater (PuWW) (11.7% CDW) and mixed wastewater (MWW) (8.2% CDW) after seven days of cultivation. The lipids mostly contained C16-C18 fatty acids groups with oleic acid and palmitic acid being the dominant fatty acids. The maximum biodiesel yield was about 6-8% CDW for all the wastewaters. The results showed the potential of utilizing pulp and paper mill effluents and other waste streams, such as activated sludge for the sustainable production of lipids for biofuel production.
authors.affiliation: Dave C. Swalm School of Chemical Engineering, Mississippi State University, MS 39762, USA.
authors.forename: Kamal Lamichhane
authors.initials: KL
authors.lastname: Upadhyaya
authors.affiliation: Department of Chemical and Paper Engineering, Western Michigan University, Kalamazoo, MI 49008, USA.
authors.forename: Andro
authors.initials: A
authors.lastname: Mondala
authors.affiliation: Department of Chemical Engineering, University of Louisiana at Lafayette, Lafayette, LA 70504, USA.
authors.forename: Rafael
authors.initials: R
authors.lastname: Hernandez
authors.affiliation: Dave C. Swalm School of Chemical Engineering, Mississippi State University, MS 39762, USA.
authors.forename: Todd
authors.initials: T
authors.lastname: French
authors.affiliation: Dave C. Swalm School of Chemical Engineering, Mississippi State University, MS 39762, USA.
authors.forename: Magan
authors.initials: M
authors.lastname: Green
authors.affiliation: Dave C. Swalm School of Chemical Engineering, Mississippi State University, MS 39762, USA.
authors.forename: Linda
authors.initials: L
authors.lastname: McFarland
authors.affiliation: Department of Chemical Engineering, University of Louisiana at Lafayette, Lafayette, LA 70504, USA.
authors.forename: William
authors.initials: W
authors.lastname: Holmes
mesh_terms: D056804:Biofuels; D005227:Fatty Acids; D005285:Fermentation; D007220:Industrial Waste; D007700:Kinetics; D010209:Paper; D012722:Sewage; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater; D014934:Wood
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D007220:Industrial Waste; D012722:Sewage; D062065:Wastewater