title: A multi-modality platform to image stem cell graft survival in the naïve and stroke-damaged mouse brain.
abstract: Neural stem cell implantations have been extensively investigated for treatment of brain diseases such as stroke. In order to follow the localization and functional status of cells after implantation noninvasive imaging is essential. Therefore, we developed a comprehensive multi-modality platform for in vivo imaging of graft localization, density, and survival using 19F magnetic resonance imaging in combination with bioluminescence imaging. We quantitatively analyzed cell graft survival over the first 4 weeks after transplantation in both healthy and stroke-damaged mouse brain and correlated our findings of graft vitality with the host innate immune response. The multi-modality imaging platform will help to improve cell therapy also in context other than stroke and to gain indispensable information for clinical translation.
authors.affiliation: In-Vivo-NMR Laboratory, Max-Planck-Institute for Neurological Research in Cologne, Cologne, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Boehm-Sturm
authors.affiliation: In-Vivo-NMR Laboratory, Max-Planck-Institute for Neurological Research in Cologne, Cologne, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Aswendt
authors.affiliation: In-Vivo-NMR Laboratory, Max-Planck-Institute for Neurological Research in Cologne, Cologne, Germany.
authors.forename: Anuka
authors.initials: A
authors.lastname: Minassian
authors.affiliation: In-Vivo-NMR Laboratory, Max-Planck-Institute for Neurological Research in Cologne, Cologne, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Michalk
authors.affiliation: In-Vivo-NMR Laboratory, Max-Planck-Institute for Neurological Research in Cologne, Cologne, Germany.
authors.forename: Luam
authors.initials: L
authors.lastname: Mengler
authors.affiliation: In-Vivo-NMR Laboratory, Max-Planck-Institute for Neurological Research in Cologne, Cologne, Germany.
authors.forename: Joanna
authors.initials: J
authors.lastname: Adamczak
authors.affiliation: Department of Radiology, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Laura
authors.initials: L
authors.lastname: Mezzanotte
authors.affiliation: Department of Radiology, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Clemens
authors.initials: C
authors.lastname: Löwik
authors.affiliation: In-Vivo-NMR Laboratory, Max-Planck-Institute for Neurological Research in Cologne, Cologne, Germany; Department of Radiology, Leiden University Medical Center, Leiden, The Netherlands. Electronic address: mathias@nf.mpg.de.
authors.forename: Mathias
authors.initials: M
authors.lastname: Hoehn
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology*; D006085:Graft Survival; D008297:Male; D051379:Mice; D008819:Mice, Nude; D033581:Stem Cell Transplantation; D020521:Stroke / Q000473:pathology*
keywords: 19F-MRI; BLI; MCAO; Neural stem cells; Stem cell implantation; Stroke