title: Determination of three nitroimidazoles in rabbit plasma by two-step stacking in capillary zone electrophoresis featuring sweeping and micelle to solvent stacking.
abstract: This study reports a sweeping and micelle to solvent stacking (MSS) method for fast stacking of nitroimidazoles in capillary zone electrophoresis (CZE). The optimal experimental conditions are run buffer of 25mM sodium phosphate (pH 1.5) and 0.50% (v/v) methanol, micellar solution of 30mM sodium dodecyl sulfate, applied voltage of 28kV, and sample and micellar solution injection of 100s. By applying this new on-line preconcentration technique, the nitroimidazoles content can be determined within 9min with the limit of detection (S/N=3) ranging 2.3-3.0ng/mL, which is lower than that of conventional CZE analysis. The proposed MSS technique affords 20-, 12- and 38-fold improvements in sensitivity for the detection of dimetridazole, metronidazole and secnidazole, respectively. The relative standard deviations (RSDs) of intra-day and inter-day are 2.1-3.6% and 2.7-4.6% (n=6), respectively. The recoveries in pretreated rabbit plasma at spiked levels of 5.0-10.0μg/mL are 92.0-101.1% with RSDs lower than 3.4%. The proposed sweeping and MSS-CZE is a highly sensitive method for the detection of nitroimidazoles in biological and clinical samples and has been successfully applied to analyze nitroimidazoles in pretreated rabbit plasma.
authors.affiliation: College of Chemistry and Chemical Engineering, Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, China West Normal University, Nanchong 637000, China. Electronic address: xiupeiyang@163.com.
authors.forename: Xiupei
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Chemistry and Chemical Engineering, Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, China West Normal University, Nanchong 637000, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Cheng
authors.affiliation: College of Chemistry and Chemical Engineering, Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, China West Normal University, Nanchong 637000, China.
authors.forename: Yingyun
authors.initials: Y
authors.lastname: Lin
authors.affiliation: College of Chemistry and Chemical Engineering, Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, China West Normal University, Nanchong 637000, China.
authors.forename: Zhijing
authors.initials: Z
authors.lastname: Tan
authors.affiliation: College of Chemistry and Chemical Engineering, Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, China West Normal University, Nanchong 637000, China.
authors.forename: Linxiang
authors.initials: L
authors.lastname: Xie
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, 224 Waterloo Road, Kowloon Tong, Hong Kong Special Administrative Region. Electronic address: mfchoi@hkbu.edu.hk.
authors.forename: Martin M F
authors.initials: MM
authors.lastname: Choi
mesh_terms: D000818:Animals; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D000432:Methanol / Q000737:chemistry; D008823:Micelles; D009593:Nitroimidazoles / Q000097:blood* / Q000737:chemistry; D011817:Rabbits; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
chemical_list: D002021:Buffers; D008823:Micelles; D009593:Nitroimidazoles; D012997:Solvents; D012967:Sodium Dodecyl Sulfate; D000432:Methanol
keywords: Capillary electrophoresis; Micelle to solvent stacking; Nitroimidazole; Rabbit plasma; Sweeping