title: Identification of the cis-element and bZIP DNA binding motifs for the autogenous negative control of mouse NOSTRIN.
abstract: mNOSTRIN is the mouse ortholog of hNOSTRIN. Unlike hNOSTRIN, which is alternatively spliced to produce two isoforms (α and β), only a single isoform of mNOSTRIN has been detected in either the nucleus or cytoplasm/membrane. Because mNOSTRIN represses its own transcription through direct binding onto its own promoter, this protein is constantly expressed in a temporally regulated pattern during differentiation of F9 embryonic carcinoma cells. In this study, we identified the specific cis-element in the mNOSTRIN regulatory region that is responsible for negative autogenous control. This element exhibits inverted dyad symmetry. Furthermore, we identified a putative bZIP motif in the middle region of mNOSTRIN, which is responsible for DNA binding, and showed that disruption of the leucine zippers abolished the DNA-binding activity of mNOSTRIN. Here, we report that a single form of mNOSTRIN functions in both the nucleus and cytoplasm/membrane. In the nucleus, mNOSTRIN acts as a transcriptional repressor by binding to the cis-element through its bZIP motif.
authors.affiliation: Department of Life Science, Korea University, 5-ka, Anam-Dong, Sungbuk-ku, Seoul 136-701, Republic of Korea.
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Bae
authors.affiliation: Department of Life Science, Korea University, 5-ka, Anam-Dong, Sungbuk-ku, Seoul 136-701, Republic of Korea.
authors.forename: Young-Joon
authors.initials: YJ
authors.lastname: Choi
authors.affiliation: Department of Life Science, Korea University, 5-ka, Anam-Dong, Sungbuk-ku, Seoul 136-701, Republic of Korea.
authors.forename: Kyung-hyun
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Department of Life Science, Korea University, 5-ka, Anam-Dong, Sungbuk-ku, Seoul 136-701, Republic of Korea. Electronic address: sspark@korea.ac.kr.
authors.forename: Sung-Soo
authors.initials: SS
authors.lastname: Park
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D019556:COS Cells; D002454:Cell Differentiation / Q000235:genetics; D002522:Chlorocebus aethiops; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D055029:Inverted Repeat Sequences / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D050976:Basic-Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; C495611:Nostrin protein, mouse; D004247:DNA
keywords: Inverted-repeat; NOSTRIN; bZIP