title: Expression of Panton-Valentine leukocidin mRNA among Staphylococcus aureus isolates associates with specific clinical presentations.
abstract: Panton-Valentine leukocidin (PVL; gene designation lukF/S-PV) is likely an important virulence factor for Staphylococcus aureus (S. aureus), as qualitative expression of the protein correlates with severity for specific clinical presentations, including skin and soft tissue infections (SSTIs). Development of genetic approaches for risk-assessment of patients with S. aureus infections may prove clinically useful, and whether lukF/S-PV gene expression correlates with specific clinical presentations for S. aureus has been largely unexplored. In the present study, we quantified lukS-PV mRNA among 96 S. aureus isolates to determine whether expression levels correlated with specific clinical presentations in adults and children. Expression level of lukS-PV mRNA among isolates from skin and soft tissue infections (SSTIs) was significantly greater than among isolates from blood stream infection (BSIs), and expression level of lukS-PV mRNA among BSI isolates from children was significantly greater than for BSI isolates among adults. Moreover, expression level of lukS-PV mRNA among community-acquired (CA) isolates was significantly greater than for hospital-acquired (HA) isolates. These data justify additional studies to determine the potential clinical utility for lukS-PV mRNA quantification as a predictive tool for severity of S. aureus infection.
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou, Medical University, Wenzhou, Zhejiang, China.
authors.forename: Fangyou
authors.initials: F
authors.lastname: Yu
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou, Medical University, Wenzhou, Zhejiang, China.
authors.forename: Yongpeng
authors.initials: Y
authors.lastname: Shang
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Danping
authors.initials: D
authors.lastname: Lou
authors.affiliation: Departments of Medicine and Microbiology, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Qin
authors.affiliation: Departments of Medicine and Microbiology, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America.
authors.forename: Chris
authors.initials: C
authors.lastname: Parsons
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou, Medical University, Wenzhou, Zhejiang, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.
authors.forename: Longhua
authors.initials: L
authors.lastname: Hu
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Liangxing
authors.initials: L
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002648:Child; D002675:Child, Preschool; D005098:Exotoxins / Q000096:biosynthesis*; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007049:Iatrogenic Disease; D007956:Leukocidins / Q000096:biosynthesis*; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification* / Q000378:metabolism*; D012329:RNA, Bacterial / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis*; D013203:Staphylococcal Infections / Q000378:metabolism / Q000382:microbiology
chemical_list: D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D012329:RNA, Bacterial; D012333:RNA, Messenger