title: RefPrimeCouch--a reference gene primer CouchApp.
abstract: To support a quantitative real-time polymerase chain reaction standardization project, a new reference gene database application was required. The new database application was built with the explicit goal of simplifying not only the development process but also making the user interface more responsive and intuitive. To this end, CouchDB was used as the backend with a lightweight dynamic user interface implemented client-side as a one-page web application. Data entry and curation processes were streamlined using an OpenRefine-based workflow. The new RefPrimeCouch database application provides its data online under an Open Database License. Database URL: http://hpclife.th-wildau.de:5984/rpc/_design/rpc/view.html.
authors.affiliation: Department of Molecular Biotechnology and Functional Genomics, University of Applied Sciences Wildau, 15745 Wildau, Germany and High Performance Computing in Life Sciences/Bioinformatics, University of Applied Sciences Wildau, 15745 Wildau, Germany.
authors.forename: Jascha
authors.initials: J
authors.lastname: Silbermann
authors.forename: Catrin
authors.initials: C
authors.lastname: Wernicke
authors.forename: Heike
authors.initials: H
authors.lastname: Pospisil
authors.forename: Marcus
authors.initials: M
authors.lastname: Frohme
mesh_terms: D017931:DNA Primers / Q000378:metabolism*; D030541:Databases, Genetic / Q000592:standards*; D020407:Internet; D012015:Reference Standards; D013223:Statistics as Topic; D014584:User-Computer Interface
chemical_list: D017931:DNA Primers