title: [Isolation of a methanol-utilizing strain and its application for determining methanol].
abstract: OBJECTIVE
  To isolate and characterize bacteria that can be used todevelop microbial biosensor for methanol (MeOH) determination.
  
  
  METHODS
  We used selective medium and streak plate to isolate bacteria. Morphological, physiological characteristics and 16S rDNA sequence analysis were used to identify the strain. An MeOH biosensor was then developed by immobilizing M211 along with dissolved oxygen (O2) sensor.
  
  
  RESULTS
  An MeOH utilizing bacterium was isolated from biogas-producing tank using methanol as the sole carbon source, and identified as Methylobacteriumorganophilium. Decrease of O2 concentration is linearly related to the MeOH concentration in the range from 0.02% to 1%, with the MeOH detection limit of 0.27 mg/L. The response time of the biosensor is within 20 min. Furthermore, the result of interference test and the detection of methanol sample are both satisfactory.
  
  
  CONCLUSIONS
  Good results are obtained in interference test and the detection of methanol sample. The proposed method seems very attractive in monitoring methanol.
authors.affiliation: Institute of Agricultural Environment & Resource, Shanxi Academy of Agricultural Sciences, Taiyuan 030006, China. 13935163790@163.com
authors.forename: Jun
authors.initials: J
authors.lastname: Guo
authors.affiliation: Wheat Research Institute Agricultural Sciences, Linfen 041000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.affiliation: College of Life Sciences, Shanxi Normal University, Linfen 041004, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Wheat Research Institute Agricultural Sciences, Linfen 041000, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Qu
authors.affiliation: Wheat Research Institute Agricultural Sciences, Linfen 041000, China.
authors.forename: Dongtao
authors.initials: D
authors.lastname: Lu
authors.affiliation: Wheat Research Institute Agricultural Sciences, Linfen 041000, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Institute of Agricultural Environment & Resource, Shanxi Academy of Agricultural Sciences, Taiyuan 030006, China.
authors.forename: Jinmei
authors.initials: J
authors.lastname: Pang
authors.affiliation: Wheat Research Institute Agricultural Sciences, Linfen 041000, China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Yang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D000432:Methanol / Q000032:analysis* / Q000378:metabolism*; D020580:Methylobacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D010100:Oxygen / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D010100:Oxygen; D000432:Methanol