title: The effects of anastrozole on the proliferation of FM3A cells.
abstract: OBJECTIVE
  In this study, the antiproliferative effects of the aromatase inhibitor anastrozole (arimidex®) was evaluated on estrogen receptor (ER) positive FM3A cell line originated from C3H mouse mammary carcinoma.
  
  
  METHODS
  For this purpose cell kinetic parameters including viability analysis, mitotic index and labelling index were used. Three different doses of anostrozole (D1= 0.01 μM, D2= 0.1μM, D3= 1μM) were applied to cells for 24 h to determine the most effective dose. A dose of 1μM dose was determined as the most effective and this was used in all subsequent applications for 0-72 h.
  
  
  RESULTS
  The results showed that there was a significant decrease in viability, mitotic index and labelling index for all experimental groups. The differences between control and all experimental groups were statistically significant (p<0.01) for all applications.
  
  
  CONCLUSIONS
  Anastrozole offers a promising treatment modality in estrogen sensitive breast cancer.
authors.affiliation: Istanbul University, Faculty of Science, Department of Biology, Istanbul, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Topcul
authors.forename: I
authors.initials: I
authors.lastname: Cetin
authors.forename: M
authors.initials: M
authors.lastname: Ozlem Kolusayin Ozar
mesh_terms: D000077384:Anastrozole; D000818:Animals; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology*; D047072:Aromatase Inhibitors / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D051379:Mice; D008809:Mice, Inbred C3H; D008940:Mitotic Index; D009376:Neoplasms, Hormone-Dependent / Q000378:metabolism / Q000473:pathology*; D009570:Nitriles / Q000494:pharmacology*; D011960:Receptors, Estrogen / Q000378:metabolism; D013997:Time Factors; D014230:Triazoles / Q000494:pharmacology*
chemical_list: D018931:Antineoplastic Agents, Hormonal; D047072:Aromatase Inhibitors; D009570:Nitriles; D011960:Receptors, Estrogen; D014230:Triazoles; D000077384:Anastrozole