title: Neurotrophic and neuroprotective actions of Achyranthes bidentata polypeptides on cultured dorsal root ganglia of rats and on crushed common peroneal nerve of rabbits.
abstract: We have isolated Achyranthes bidentata Blume polypeptides (ABPP) from the aqueous extract of A. bidentata Blume, a traditional Chinese medicine with multiple therapeutic applications. In this study, we aimed to investigate neurotrophic effects of ABPP on cultured dorsal root ganglia (DRGs) of rats and neuroprotective effects on crushed common peroneal nerve of rabbits. Immunochemistry and Western blot analysis indicated that ABPP (0.01, 0.1, and 1.0 μg/ml) encouraged neurite outgrowth from cultured DRG explants/neurons in a concentration-dependent manner through activation of ERK1/2. After crush injury to rabbit common peroneal nerve, animals received daily administration of ABPP for 5 weeks. Electrophysiological assessments and histomorphological evaluation showed that 6.0mg/kg ABPP significantly enhanced nerve regeneration and function restoration. Our findings suggest that ABPP could be used as a neurotrophic and neuroprotective agent to treat peripheral nerve crush injury.
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, Jiangsu 226001, PR China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Cheng
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, Jiangsu 226001, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, Jiangsu 226001, PR China.
authors.forename: Changnan
authors.initials: C
authors.lastname: Sun
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, Jiangsu 226001, PR China.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Gu
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, Jiangsu 226001, PR China. Electronic address: zheng.cao@vanderbilt.edu.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, Jiangsu 226001, PR China. Electronic address: dingfei@ntu.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Ding
mesh_terms: D029044:Achyranthes; D000818:Animals; D000831:Animals, Newborn; D005727:Ganglia, Spinal / Q000187:drug effects*; D008516:Medicine, Chinese Traditional; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010543:Peroneal Nerve / Q000187:drug effects* / Q000293:injuries; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000187:drug effects / Q000293:injuries; D046509:Tissue Culture Techniques
chemical_list: D018696:Neuroprotective Agents; D010936:Plant Extracts
keywords: Achyranthes bidentata Blume polypeptides; Crush injury; Dorsal root ganglion; Neurite outgrowth; Peripheral nerve regeneration