title: Expression and genomic imprinting of the porcine Rasgrf1 gene.
abstract: Imprinted genes play important roles in mammalian growth, development and behavior. The Rasgrf1 (Ras protein-specific guanine nucleotide exchange factor 1) gene has been identified as an imprinted gene in mouse and rat. In the present study, we detected its sequence, imprinting status and expression pattern in the domestic pigs. A 228 bp partial sequence located in exon 14 and a 193 bp partial sequence located in exon 1 of the Rasgrf1 gene in domestic pigs were obtained. A G/A transition, was identified in Rasgrf1 exon 14, and then, the reciprocal Berkshire × Wannan black F1 hybrid model and the RT-PCR-RFLP method were used to detect the imprinting status of porcine Rasgrf1 gene at the developmental stage of 1-day-old. The expression profile results indicated that the porcine Rasgrf1 mRNA was highly expressed in brain, pituitary and pancreas, followed by kidney, stomach, lung, testis, small intestine, ovary, spleen and liver, and at low levels of expression in longissimus dorsi, heart, and backfat. The expression levels of Rasgrf1 gene in brain, pituitary and pancreas tissues were significantly different between the two reciprocal F1 hybrids. Imprinting analysis showed that porcine Rasgrf1 gene was maternally expressed in the liver, small intestine, paternally expressed in the lung, but biallelically expressed in brain, heart, spleen, kidney, stomach, pancreas, backfat, testis, ovary, longissimus dorsi and pituitary tissues.
authors.affiliation: Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui 230036, PR China.
authors.forename: Yue-Yun
authors.initials: YY
authors.lastname: Ding
authors.affiliation: Department of Veterinary, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui 230036, PR China.
authors.forename: Li-Yuan
authors.initials: LY
authors.lastname: Liu
authors.affiliation: Department of Veterinary, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui 230036, PR China. Electronic address: zhoujie@ahau.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui 230036, PR China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Zhang
authors.affiliation: Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui 230036, PR China.
authors.forename: Long
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Veterinary, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui 230036, PR China.
authors.forename: Shu-Jing
authors.initials: SJ
authors.lastname: Zhang
authors.affiliation: Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui 230036, PR China. Electronic address: yinzongjun@ahau.edu.cn.
authors.forename: Zong-Jun
authors.initials: ZJ
authors.lastname: Yin
mesh_terms: D000483:Alleles; D000818:Animals; D005091:Exons / Q000235:genetics; D018392:Genomic Imprinting / Q000235:genetics*; D034421:Sus scrofa / Q000235:genetics*; D020666:ras-GRF1 / Q000235:genetics*
chemical_list: D020666:ras-GRF1
keywords: 3/4rd; ANOVA; BLAST; CCCTC-binding factor; CTCF; DMD; Domestic pigs; ESTs; Expression; F(1); GH/IGF1 axis; Genomic imprinting; LSD; MAPK; QTL; RT-PCR-RFLP; Ras protein-specific guanine nucleotide exchange factor 1; Rasgrf1; SNP; SSC; Sus scrofa chromosome; analysis of variance; base pair; basic local alignment search tool; between the third and fourth; bp; cDNA; cM; centi-Morgan; complementary deoxyribonucleic acid; differentially methylated domain; expressed sequence tags; growth hormone/insulin-like growth factor 1 axis; least-significant difference; mRNA; messenger ribonucleic acid; mitogen-activated protein kinases; quantitative trait loci; reverse transcription-polymerase chain reaction-restricted fragment length polymorphism; single nucleotide polymorphism; the first filial generation