title: Increased expression of the adult stem cell marker Musashi-1 in the ectopic endometrium of adenomyosis does not correlate with serum estradiol and progesterone levels.
abstract: OBJECTIVE
  To investigate whether abnormal expression of Musashi-1 occurs in eutopic and ectopic endometria from patients with adenomyosis and whether it is correlated with serum estradiol or progesterone levels.
  
  
  METHODS
  Musashi-1 expression in ectopic and paired eutopic endometrium of patients with adenomyosis (adenomyosis group, 30 cases) and in normal endometrium (control group, 29 cases) was studied with immunohistochemistry, and serum estradiol and progesterone levels in these cases were measured with electrochemiluminescence immunoassay.
  
  
  RESULTS
  In all the normal endometrial tissues from control group and paired eutopic and ectopic endometrium of adenomyosis, epithelial and stromal Musashi-1 expression was observed throughout the menstrual cycle. Musashi-1 immunostaining was localized both in the nucleus and cytoplasm. The epithelial cells in normal endometrium showed a significantly higher staining index of Musashi-1 in the proliferative phase than in the secretory phase. The staining index of epithelial Musashi-1 in the ectopic endometrium was significantly higher than in the paired eutopic endometrium and normal endometrium throughout the menstrual cycle. In the secretory phase, the epithelial Musashi-1 immunoreactivity in eutopic endometrium and the stromal Musashi-1 immunoreactivity in ectopic endometrium were significantly higher than those in the normal endometrium. The Musashi-1 expression in the ectopic endometrium of adenomyosis was not correlated with the serum estradiol or progesterone levels.
  
  
  CONCLUSIONS
  Aberrant Musashi-1 expression in adenomyosis may be involved in the pathogenesis and pathophysiology of adenomyosis, but it does not correlate with changes in serum estradiol or progesterone levels.
authors.affiliation: Reproductive Medical Center, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, PR China; Department of Gynaecology and Obstetrics, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, PR China.
authors.forename: Yue-zhou
authors.initials: YZ
authors.lastname: Chen
authors.affiliation: Department of Gynaecology and Obstetrics, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, PR China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Wang
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Shanxi Medical University, Taiyuan, 030001, PR China. Electronic address: yanjun2005@126.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Pathology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Pathology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, PR China.
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhou
mesh_terms: D062788:Adenomyosis / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D004958:Estradiol / Q000097:blood*; D005260:Female; D006801:Humans; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011374:Progesterone / Q000097:blood*; D016601:RNA-Binding Proteins / Q000378:metabolism*
chemical_list: C491297:MSI1 protein, human; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins; D011374:Progesterone; D004958:Estradiol
keywords: Adenomyosis; Endometrium; Estradiol; Musashi-1; Progesterone