title: Lysine-appended polydiacetylene scaffolds for human mesenchymal stem cells.
abstract: We report on the self-assembly based fabrication of fibrous polymers for tissue engineering applications. Directed self-assembly followed by polymerization of lysine-appended diacetylenes generated a variety of polymers (P1-P5) with distinct chemical properties. The self-assembly along with the conjugated double and triple bonds and rigid geometry of diacetylene backbone imposed a nanofibrous morphology on the resulting polymers. Chemical properties including wettability of the polymers were tuned by using lysine (Lys) with orthogonal protecting groups (Boc and Fmoc). These Lys-appended polydiacetylene scaffolds were compared in terms of their efficiency toward human mesenchymal stem cells adhesion and spreading. Interestingly, polymer P4 containing Lys N(α)-NH2 and Lys N(ε)-Boc with balanced wettability supported cell adhesion better than the more hydrophobic polymer P2 with N(ε)-Boc and N(α)-Fmoc or more hydrophilic polymer P5 containing free N(ε) and N(α) amino groups. The molecular level control in the fabrication of nanofibrous polymers compared with other existing methods for the generation of fibrous polymers is the hallmark of this work.
authors.affiliation: Department of Chemistry, Indian Institute of Technology Delhi , New Delhi-110016, India.
authors.forename: V
authors.initials: V
authors.lastname: Haridas
authors.forename: Sandhya
authors.initials: S
authors.lastname: Sadanandan
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Collart-Dutilleul
authors.forename: Stan
authors.initials: S
authors.lastname: Gronthos
authors.forename: Nicolas H
authors.initials: NH
authors.lastname: Voelcker
mesh_terms: D002465:Cell Movement; D002470:Cell Survival; D006801:Humans; D008239:Lysine / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000737:chemistry / Q000166:cytology*; D008968:Molecular Conformation; D010316:Particle Size; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene; D008239:Lysine