title: Rapid matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging with scanning desorption laser beam.
abstract: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) imaging of surfaces and tissues is a rapidly evolving technique having great potential in the field of biosciences. In earlier times, acquisition of a single high-resolution MS image could take days. Despite the recent introduction of high-repetition rate lasers to increase sample throughput of axial TOF MS instruments, obtaining a high-resolution image still requires a few hours. This paper shows that a substantial increase in the throughput of the TOF MS-based tissue imaging can be achieved by incorporating a mirror providing high-speed precision scanning of the laser beam along the sample surface. Equipped with the scanning mirror, a laboratory-built axial MALDI TOF MS instrument utilizing a 4-kHz UV laser recorded a 100 × 100 pixel MS image in ~11 min using 100 laser shots per pixel. This is almost an order of magnitude faster when compared to a modern commercial instrument equipped with 1-kHz laser.
authors.affiliation: Central European Institute of Technology (CEITEC), Masaryk University , 625 00 Brno, Czech Republic.
authors.forename: Antonín
authors.initials: A
authors.lastname: Bednařík
authors.forename: Pavel
authors.initials: P
authors.lastname: Kuba
authors.forename: Eugene
authors.initials: E
authors.lastname: Moskovets
authors.forename: Iva
authors.initials: I
authors.lastname: Tomalová
authors.forename: Pavel
authors.initials: P
authors.lastname: Krásenský
authors.forename: Pavel
authors.initials: P
authors.lastname: Houška
authors.forename: Jan
authors.initials: J
authors.lastname: Preisler
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000032:analysis; D004867:Equipment Design; D005841:Gentisates / Q000737:chemistry; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007834:Lasers; D008027:Light; D010455:Peptides / Q000032:analysis* / Q000138:chemical synthesis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods
chemical_list: D005841:Gentisates; D010455:Peptides; D000324:Adrenocorticotropic Hormone; C010925:2,5-dihydroxybenzoic acid