title: Peripheral nerve regeneration following transection injury to rat sciatic nerve by local application of adrenocorticotropic hormone.
abstract: The objective of this study was to assess local effect of adrenocorticotropic hormone (ACTH) on the functional recovery of the sciatic nerve in a transection model. Sixty male healthy white Wistar rats were randomized into four experimental groups of 15 animals each: In the sham-operated group (SHAM), the sciatic nerve was exposed and manipulated. In the transected group (TC), the left sciatic nerve was transected and the cut nerve ends were fixed in the adjacent muscle. In the silicone graft group (SIL) a 10-mm defect was made and bridged using a silicone tube. The graft was filled with phosphated-buffer saline alone. In the treatment group a silicone tube (SIL/ACTH) was filled with 10 μL ACTH (0.1 mg/mL). Each group was subdivided into three subgroups of five animals each and regenerated nerve fibres were studied at 4, 8 and 12 weeks post operation. Behavioral testing, functional, gastrocnemius muscle mass and morphometric indices showed earlier regeneration of axons in SIL/ACTH than in SIL group (p < 0.05). Immunohistochemistry clearly showed more positive location of reactions to S-100 in SIL/ACTH than in SIL group. ACTH improved functional recovery and morphometric indices of sciatic nerve. This finding supports role of ACTH after peripheral nerve repair and may have clinical implications for the surgical management of patients after nerve transection.
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Nazloo Road, Urmia 57153 1177, Iran. Electronic address: r.mohammadi@urmia.ac.ir.
authors.forename: Rahim
authors.initials: R
authors.lastname: Mohammadi
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
authors.forename: Mohammad-Javad
authors.initials: MJ
authors.lastname: Yadegarazadi
authors.affiliation: Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4, Canada.
authors.forename: Keyvan
authors.initials: K
authors.lastname: Amini
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000627:therapeutic use*; D000818:Animals; D001369:Axons / Q000648:ultrastructure; D048091:Guided Tissue Regeneration / Q000295:instrumentation; D008297:Male; D018482:Muscle, Skeletal / Q000294:innervation / Q000473:pathology; D009186:Myelin Sheath / Q000648:ultrastructure; D009412:Nerve Fibers / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000187:drug effects*; D009929:Organ Size; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000187:drug effects; D009418:S100 Proteins / Q000032:analysis; D012584:Sciatic Nerve / Q000187:drug effects* / Q000473:pathology / Q000601:surgery; D012828:Silicones / Q000737:chemistry; D013997:Time Factors; D054457:Tissue Scaffolds / Q000737:chemistry; D016138:Walking / Q000502:physiology
chemical_list: D009418:S100 Proteins; D012828:Silicones; D000324:Adrenocorticotropic Hormone
keywords: ACTH; Local; Nerve repair; Sciatic