title: Adult neural stem cells in distinct microdomains generate previously unknown interneuron types.
abstract: Throughout life, neural stem cells (NSCs) in different domains of the ventricular-subventricular zone (V-SVZ) of the adult rodent brain generate several subtypes of interneurons that regulate the function of the olfactory bulb. The full extent of diversity among adult NSCs and their progeny is not known. Here, we report the generation of at least four previously unknown olfactory bulb interneuron subtypes that are produced in finely patterned progenitor domains in the anterior ventral V-SVZ of both the neonatal and adult mouse brain. Progenitors of these interneurons are responsive to sonic hedgehog and are organized into microdomains that correlate with the expression domains of the Nkx6.2 and Zic family of transcription factors. This work reveals an unexpected degree of complexity in the specification and patterning of NSCs in the postnatal mouse brain.
authors.affiliation: 1] Department of Neurological Surgery, University of California, San Francisco, San Francisco, California, USA. [2] Eli and Edythe Broad Center of Regeneration, Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, California, USA. [3] Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts, USA. [4] Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA. [5] Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts, USA. [6].
authors.forename: Florian T
authors.initials: FT
authors.lastname: Merkle
authors.affiliation: 1] Department of Neurological Surgery, University of California, San Francisco, San Francisco, California, USA. [2] Eli and Edythe Broad Center of Regeneration, Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, California, USA. [3].
authors.forename: Luis C
authors.initials: LC
authors.lastname: Fuentealba
authors.affiliation: 1] Department of Neurological Surgery, University of California, San Francisco, San Francisco, California, USA. [2] Eli and Edythe Broad Center of Regeneration, Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, California, USA.
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Sanders
authors.affiliation: 1] Wolfson Institute for Biomedical Research, University College London, Gower Street, London, UK. [2] Department of Cell and Developmental Biology, University College London, Gower Street, London, UK.
authors.forename: Lorenza
authors.initials: L
authors.lastname: Magno
authors.affiliation: 1] Wolfson Institute for Biomedical Research, University College London, Gower Street, London, UK. [2] Department of Cell and Developmental Biology, University College London, Gower Street, London, UK. [3].
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Kessaris
authors.affiliation: 1] Department of Neurological Surgery, University of California, San Francisco, San Francisco, California, USA. [2] Eli and Edythe Broad Center of Regeneration, Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, California, USA. [3].
authors.forename: Arturo
authors.initials: A
authors.lastname: Alvarez-Buylla
mesh_terms: D000367:Age Factors; D000818:Animals; D002552:Cerebral Ventricles / Q000166:cytology / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D007395:Interneurons / Q000145:classification* / Q000502:physiology*; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism; D021962:Membrane Microdomains / Q000502:physiology*; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology*; D009457:Neuroglia / Q000502:physiology; D022661:RNA, Untranslated / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014161:Transduction, Genetic; D000071676:Zinc Finger Protein GLI1
chemical_list: C498520:Gli1 protein, mouse; D005904:Glial Fibrillary Acidic Protein; C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D018398:Homeodomain Proteins; D051741:Kruppel-Like Transcription Factors; C436827:Nkx6.2 protein, vertebrate; D022661:RNA, Untranslated; D014157:Transcription Factors; C492122:Zic1 protein, mouse; D000071676:Zinc Finger Protein GLI1; D049452:Green Fluorescent Proteins