title: Anti-HPV-E7 seropositivity and risk of esophageal squamous cell carcinoma in a high-risk population in China.
abstract: Results of previous serologic studies on the association of human papillomavirus (HPV) with esophageal squamous cell carcinoma (ESCC) have been inconsistent. From 2007 to 2010, the authors collected blood samples and relevant demographic data from 1435 patients with ESCC and 2071 age- and sex-matched normal controls from Anyang, China. HPV-16, 18 and 57 E7 antibodies were evaluated with the glutathione-S-transferase capture ELISA. The proportions of subjects who were positive for antibodies against these three HPV antigens in the case group were all significantly higher than those in the control group. In multivariate analysis, the presence of HPV-16 E7 antibody was associated with an increased risk of ESCC [odds ratio (OR) = 3.6, 95% confidence interval (CI): 2.5-5.0], whereas the presence of HPV-18 (OR = 1.1, 95% CI: 0.7-1.7) and HPV-57 (OR = 1.3, 95% CI: 0.9-1.9) antibodies were not significant after adjustment for HPV-16. In multiple cutoff value analysis, the lowest OR for HPV-16 was obtained with the standard cut point mean + 3 SD. This study provides serological evidence in support of HPV-16 infection playing a role in the occurrence of ESCC in a high-incidence area of China.
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Laboratory of Genetics, Peking University Cancer Hospital & Institute, No. 52 Fucheng Road, Beijing 100142, P.R. China.
authors.forename: Zhonghu
authors.initials: Z
authors.lastname: He
authors.forename: Zhongyao
authors.initials: Z
authors.lastname: Xu
authors.forename: Dong
authors.initials: D
authors.lastname: Hang
authors.forename: Fangcen
authors.initials: F
authors.lastname: Guo
authors.forename: Amir
authors.initials: A
authors.lastname: Abliz
authors.forename: Noel S
authors.initials: NS
authors.lastname: Weiss
authors.forename: Longfu
authors.initials: L
authors.lastname: Xi
authors.forename: Fangfang
authors.initials: F
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Ning
authors.forename: Yaqi
authors.initials: Y
authors.lastname: Pan
authors.forename: Chuanhai
authors.initials: C
authors.lastname: Guo
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Liang
authors.forename: Changdong
authors.initials: C
authors.lastname: Lu
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Cai
authors.forename: Yang
authors.initials: Y
authors.lastname: Ke
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D052685:Alphapapillomavirus / Q000302:isolation & purification*; D002294:Carcinoma, Squamous Cell / Q000821:virology*; D002681:China / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay; D004938:Esophageal Neoplasms / Q000821:virology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012307:Risk Factors