title: Reduction of lns-1 gene expression and tissue insulin levels in n5-STZ rats.
abstract: OBJECTIVE
  The high global incidence of type 2 diabetes has challenged researchers to establish animal models that resemble the chronic stage observed in type 2 diabetes patients. One such model is induced by neonatal streptozotocin (n-STZ) administration to rat pups at 0, 2, or 5 days after birth. In this study, we assessed lns-1 gene expression and tissue insulin levels as well as serum concentration of glucose and insulin, insulin resistance, and histological changes of the islets of Langerhans in n5-STZ rats after 20-weeks post-induction.
  
  
  METHODS
  Wistar rat pups were randomly distributed into a control group and a streptozotocin-induced group. Experimental induction involved a single intraperitoneal injection of streptozotocin (150 mg/kg) into neonates at five days after birth.
  
  
  RESULTS
  At 20 weeks post-induction, streptozotocin-induced rats exhibited increased serum glucose levels, reduced serum insulin levels, impaired glucose metabolism and insulin resistance compared to control rats. Histologically, streptozotocin-induced rats exhibited atrophic islets, vacuolization, and significantly fewer insulin-positive cells. lns-1 gene expression was significantly decreased in n5-STZ rats in comparison to the control group.
  
  
  CONCLUSIONS
  Our findings support that the n5-STZ model 20 weeks post-induction represents an appropriate experimental tool to study T2D and to evaluate novel therapeutic agents and targets that involve insulin gene expression and secretion, as well as complications caused by chronic diabetes.
authors.affiliation: Instituto de Enfermedades Crónico-Degenerativas, Departamento de Biología Molecular y Genómica, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México.
authors.forename: Belinda
authors.initials: B
authors.lastname: Vargas Guerrero
authors.affiliation: Departamento de Botánica y Zoología, C.U.C.B.A, Universidad de Guadalajara, Guadalajara, Jalisco, México, pgarcia@cucba.udg.mx.
authors.forename: Pedro M
authors.initials: PM
authors.lastname: García López
authors.affiliation: Instituto de Enfermedades Crónico-Degenerativas, Departamento de Biología Molecular y Genómica, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México.
authors.forename: Ana E
authors.initials: AE
authors.lastname: González Santiago
authors.affiliation: Instituto de Enfermedades Crónico-Degenerativas, Departamento de Biología Molecular y Genómica, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México.
authors.forename: José A
authors.initials: JA
authors.lastname: Domínguez Rosales
authors.affiliation: Instituto de Enfermedades Crónico-Degenerativas, Departamento de Biología Molecular y Genómica, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México.
authors.forename: Carmen M
authors.initials: CM
authors.lastname: Gurrola Díaz
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D007328:Insulin / Q000235:genetics* / Q000378:metabolism; D007333:Insulin Resistance; D007515:Islets of Langerhans / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D013997:Time Factors
chemical_list: D007328:Insulin; D013311:Streptozocin