title: Glycation-assisted synthesized gold nanoparticles inhibit growth of bone cancer cells.
abstract: This study presents a novel approach to synthesize glycogenic gold nanoparticles (glycogenic GNps) capped with glycated products (Schiff's base, Heyns products, fructosylamine etc.). These glycogenic GNps have been found to be active against human osteosarcoma cell line (Saos-2) with an IC50 of 0.187 mM, while the normal human embryonic lung cell line (L-132) remained unaffected up to 1mM concentration. The size of glycogenic GNps can also be controlled by varying the time of incubation of gold solution. Glycation reactions involving a combination of fructose and HSA (Human Serum Albumin) were found to be effective in the reduction of gold to glycogenic GNps whereas glucose in combination with HSA did not result in the reduction of gold. The progress of the reaction was followed using UV-visible spectroscopy and NBT (Nitroblue tetrazolium) assay. The glycogenic GNps were found to be spherical in shape with an average size of 24.3 nm, in a stable emulsion. These GNps were characterized using UV-visible spectroscopy, zeta potential analysis, transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
authors.affiliation: Nanomedicine & Nanobiotechnology Lab, Department of Biosciences, Integral University, Lucknow, Uttar Pradesh 226026, India.
authors.forename: Moniba
authors.initials: M
authors.lastname: Rahim
authors.affiliation: Nanomedicine & Nanobiotechnology Lab, Department of Biosciences, Integral University, Lucknow, Uttar Pradesh 226026, India.
authors.forename: Sana
authors.initials: S
authors.lastname: Iram
authors.affiliation: Nanomedicine & Nanobiotechnology Lab, Department of Biosciences, Integral University, Lucknow, Uttar Pradesh 226026, India. Electronic address: sajid_987@rediffmail.com.
authors.forename: Mohd Sajid
authors.initials: MS
authors.lastname: Khan
authors.affiliation: Nanomedicine & Nanobiotechnology Lab, Department of Biosciences, Integral University, Lucknow, Uttar Pradesh 226026, India.
authors.forename: M Salman
authors.initials: MS
authors.lastname: Khan
authors.affiliation: Department of Environmental Sciences, EIILM University, 8th Mile Budang, Sikkim West, India.
authors.forename: Ankur R
authors.initials: AR
authors.lastname: Shukla
authors.affiliation: Nanomedicine & Nanobiotechnology Lab, Department of Biosciences, Integral University, Lucknow, Uttar Pradesh 226026, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Srivastava
authors.affiliation: Nanomedicine & Nanobiotechnology Lab, Department of Biosciences, Integral University, Lucknow, Uttar Pradesh 226026, India.
authors.forename: Saheem
authors.initials: S
authors.lastname: Ahmad
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005632:Fructose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006031:Glycosylation / Q000187:drug effects; D006046:Gold / Q000494:pharmacology*; D006801:Humans; D007211:Indoles / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D012516:Osteosarcoma / Q000188:drug therapy / Q000473:pathology*; D012709:Serum Albumin / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D055672:Static Electricity
chemical_list: D000970:Antineoplastic Agents; D007211:Indoles; D012709:Serum Albumin; D005632:Fructose; C007293:DAPI; D006046:Gold; D005947:Glucose
keywords: Glycation; Gold nanoparticles; Heyns product; L-132; Saos-2