title: Maternal deprivation decelerates postnatal morphological lung development of F344 rats.
abstract: Intensive medical care at premature born infants is often associated with separation of neonates from their mothers. Here, early artificial prolonged separation of rat pups from their dams (Maternal Deprivation, MD) was used to study potential impact on morphological lung maturation. Furthermore, we investigated the influence of an endogenous deficiency of the neuropeptide-cleaving dipeptidyl peptidase IV (DPP4), since the effects of MD are known to be partly mediated via neuropeptidergic effects, hypothesizing that MD will lead to a retardation of postnatal lung development, DPP4-dependendly. We used wild type and CD26/DPP4 deficient rats. For MD, the dam was placed each day into a separate cage for 2 h, while the pups remained in the nest on their own. Morphological lung maturation and cell proliferation at the postnatal days 7, 10, 14, and 21 were determined morphometrically. Maternally deprived wild types showed a retarded postnatal lung development compared with untreated controls in both substrains. During alveolarization, an increased thickness of alveolar septa and a decreased surface of septa about 50% were found. At the end of the morphological lung maturation, the surface of the alveolar septa was decreased at about 25% and the septal thickness remained increased about 20%. The proliferation rate was also decreased about 50% on day 14. However, the MD induced effects were less pronounced in DPP4-deficient rats, due to a significant deceleration already induced by DPP4-deficiency. Thus, MD as a model for postnatal stress experience influences remarkably postnatal development of rats, which is significantly modulated by the DPP4-system.
authors.affiliation: Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany.
authors.forename: Katharina Luise
authors.initials: KL
authors.lastname: Hupa
authors.forename: Andreas
authors.initials: A
authors.lastname: Schmiedl
authors.forename: Reinhard
authors.initials: R
authors.lastname: Pabst
authors.forename: Stephan
authors.initials: S
authors.lastname: Von Hörsten
authors.forename: Michael
authors.initials: M
authors.lastname: Stephan
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D049109:Cell Proliferation; D018819:Dipeptidyl Peptidase 4 / Q000172:deficiency / Q000502:physiology; D005260:Female; D008168:Lung / Q000166:cytology / Q000254:growth & development* / Q000502:physiology; D008426:Maternal Deprivation; D023421:Models, Animal; D008954:Models, Biological; D051381:Rats; D011916:Rats, Inbred F344; D011922:Rats, Mutant Strains; D013315:Stress, Psychological / Q000503:physiopathology*
chemical_list: D018819:Dipeptidyl Peptidase 4
keywords: CD26/DPP4; lung development; maternal deprivation; rat; stereology