title: Efficacy of antibacterial-loaded coating in an in vivo model of acutely highly contaminated implant.
abstract: OBJECTIVE
  The purpose of this study was to test the ability of DAC®, a fast resorbable, antibacterial-loaded hydrogel coating, to prevent acute bacterial colonization in an in vivo model of an intra-operatively highly contaminated implant.
  
  
  METHODS
  A histocompatibility study was performed in 10 adult New Zealand rabbits. Then, methicillin-resistant Staph. aureus were inoculated in the femur of 30 adult New Zealand rabbits at the time of intra-medullary nailing; vancomycin-loaded DAC® coated nails were compared to controls regarding local and systemic infection development.
  
  
  RESULTS
  Histocompatibility study showed no detrimental effect of DAC® hydrogel on bone tissue after 12 weeks from implant. After seven days from implant, none of the rabbits receiving vancomycin-loaded DAC® nail showed positive blood cultures, compared to all the controls; vancomycin-loaded DAC® coating was associated with local bacterial load reduction ranging from 72 to 99 %, compared to controls.
  
  
  CONCLUSIONS
  Vancomycin-loaded DAC® coating is able to significantly reduce bacterial colonization in an animal model of an intra-operatively highly contaminated implant, without local or general side effect.
authors.affiliation: Laboratory of Preclinical and Surgical Studies, Rizzoli Orthopedic Institute, Bologna, Italy, gianluca.giavaresi@ior.it.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Giavaresi
authors.forename: Enzo
authors.initials: E
authors.lastname: Meani
authors.forename: Maria
authors.initials: M
authors.lastname: Sartori
authors.forename: Andrea
authors.initials: A
authors.lastname: Ferrari
authors.forename: Davide
authors.initials: D
authors.lastname: Bellini
authors.forename: Anna C
authors.initials: AC
authors.lastname: Sacchetta
authors.forename: Joachim
authors.initials: J
authors.lastname: Meraner
authors.forename: Andrea
authors.initials: A
authors.lastname: Sambri
authors.forename: Caterina
authors.initials: C
authors.lastname: Vocale
authors.forename: Vittorio
authors.initials: V
authors.lastname: Sambri
authors.forename: Milena
authors.initials: M
authors.lastname: Fini
authors.forename: Carlo L
authors.initials: CL
authors.lastname: Romanò
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D020099:Coated Materials, Biocompatible; D004195:Disease Models, Animal; D005269:Femur / Q000382:microbiology*; D020100:Hydrogels; D008297:Male; D019736:Prostheses and Implants; D016459:Prosthesis-Related Infections / Q000188:drug therapy* / Q000601:surgery; D011817:Rabbits; D013203:Staphylococcal Infections / Q000188:drug therapy*; D013211:Staphylococcus aureus; D014640:Vancomycin / Q000008:administration & dosage*
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D020100:Hydrogels; D014640:Vancomycin