title: Effect of flavonoids on the phase separation in giant unilamellar vesicles formed from binary lipid mixtures.
abstract: Confocal fluorescence microscopy have been employed to investigate phase separation in giant unilamellar vesicles prepared from binary mixtures of unsaturated dioleoylphosphocholine with saturated phosphocholines or brain sphingomyelin in the absence and presence of the flavonoids, biochanin A, phloretin, and myricetin. It has been demonstrated that biochanin A and phloretin make uncolored domains more circular or eliminate visible phase separation in liposomes while myricetin remains the irregular shape of fluorescence probe-excluding domains. Influence of the flavonoids on the endotherms of liposome suspension composed of dioleoylphosphocholine and dimyristoylphosphocholine was investigated by the differential scanning calorimetry. Calorimetry data do not contradict to confocal imaging results.
authors.affiliation: Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky ave. 4, St. Petersburg 194064, Russia. Electronic address: osostroumova@mail.ru.
authors.forename: Olga S
authors.initials: OS
authors.lastname: Ostroumova
authors.affiliation: Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky ave. 4, St. Petersburg 194064, Russia.
authors.forename: Evgeny G
authors.initials: EG
authors.lastname: Chulkov
authors.affiliation: Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky ave. 4, St. Petersburg 194064, Russia.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Stepanenko
authors.affiliation: Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky ave. 4, St. Petersburg 194064, Russia.
authors.forename: Ludmila V
authors.initials: LV
authors.lastname: Schagina
mesh_terms: D002152:Calorimetry, Differential Scanning; D005419:Flavonoids / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D018613:Microscopy, Confocal; D010713:Phosphatidylcholines / Q000737:chemistry; D013109:Sphingomyelins / Q000737:chemistry; D044366:Transition Temperature; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D013109:Sphingomyelins; D053835:Unilamellar Liposomes
keywords: Flavonoids; Lipid bilayers; Lipid domains; Membranes; Phase separation