title: cis-Urocanic acid as a potential nickel(II) binding molecule in the human skin.
abstract: cis-Urocanic acid, a derivative of histidine, is one of the essential components of human skin. We found that it can bind nickel(II) ions in a pH-dependent manner, with the dissociation constant in the low millimolar range, as revealed by potentiometry, and confirmed by isothermal titration calorimetry and UV-vis spectroscopy. The binding occurs within the physiological skin pH range. Considering the fact that cis-urocanic acid is present in the human skin in concentrations as high as millimolar, this molecule may be a physiologically important player in nickel trafficking in the human organism.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland. tfraczyk@ibb.waw.pl.
authors.forename: Nina Ewa
authors.initials: NE
authors.lastname: Wezynfeld
authors.forename: Wojciech
authors.initials: W
authors.lastname: Goch
authors.forename: Wojciech
authors.initials: W
authors.lastname: Bal
authors.forename: Tomasz
authors.initials: T
authors.lastname: Frączyk
mesh_terms: D006639:Histidine / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000737:chemistry; D007536:Isomerism; D009532:Nickel / Q000737:chemistry* / Q000378:metabolism; D012867:Skin / Q000737:chemistry* / Q000378:metabolism; D013816:Thermodynamics; D014466:Ultraviolet Rays; D014560:Urocanic Acid / Q000737:chemistry* / Q000378:metabolism
chemical_list: D007477:Ions; D006639:Histidine; D009532:Nickel; D014560:Urocanic Acid