title: New triterpenoid saponins from Ilex cornuta and their protective effects against H2O2-induced myocardial cell injury.
abstract: Five new triterpenoid saponins, 1-5, together with 10 known ones, 6-15 were isolated from the aerial parts of Ilex cornuta. The structures of compounds 1-5 were determined as 3β-O-α-L-arabinopyranosyl-19α,23-dihydroxy-20α-urs-12-en-28-oic acid 28-O-β-D-glucopyranosyl ester, 1; 3β-O-β-D-glucopyranosyl-(1→2)-α-L-arabinopyranosyl-19-hydroxy-20α-urs-12-en-28-oic acid 28-O-β-D-glucopyranosyl ester, 2; 19α,23-dihydroxyurs-12-en-28-oic acid 3β-O-β-D-glucuronopyranoside-6-O-methyl ester, 3; 19α,23-dihydroxyurs-12-en-28-oic acid 3β-O-[β-D-glucuronopyranoside-6-O-methyl ester]-28-O-β-D-glucopyranosyl ester, 4; and 3β-O-[α-L-arabinopyranosyl-(1→2)-β-D-glucuronic acid]-oleanolic acid 28-O-β-D-glucopyranosyl ester, 5, on the basis of spectroscopic analyses (IR, ESI-MS, HR-ESI-MS, 1D and 2D NMR) and chemical reactions. Protective effects of compounds 1-15 were tested against H2O2-induced H9c2 cardiomyocyte injury, and the data showed that compounds 1, 4, 6, and 13 had significant cell-protective effects. No significant DPPH radical scavenging activity was observed for compounds 1-15.
authors.affiliation: College of Pharmaceutical Science, Soochow University , Suzhou 215123, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Li
authors.forename: Jianping
authors.initials: J
authors.lastname: Zhao
authors.forename: Yanli
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhong
authors.initials: Z
authors.lastname: Chen
authors.forename: Qiongming
authors.initials: Q
authors.lastname: Xu
authors.forename: Ikhlas A
authors.initials: IA
authors.lastname: Khan
authors.forename: Shilin
authors.initials: S
authors.lastname: Yang
mesh_terms: D002316:Cardiotonic Agents / Q000494:pharmacology*; D002460:Cell Line; D003327:Coronary Disease / Q000517:prevention & control; D016166:Free Radical Scavengers; D006861:Hydrogen Peroxide / Q000494:pharmacology; D030017:Ilex / Q000737:chemistry*; D015394:Molecular Structure; D032383:Myocytes, Cardiac / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D002316:Cardiotonic Agents; D016166:Free Radical Scavengers; D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; D006861:Hydrogen Peroxide
keywords: Aquifoliaceae; DPPH radical scavenging activity; Ilex cornuta; cardiomyocyte oxidative injury; structure identification; triterpenoid saponins