title: Antibacterial properties of recombinant human non-pancreatic secretory phospholipase A(2).
abstract: Human non-pancreatic secretory phospholipase A2 (hnpsPLA2) is a group IIA phospholipase A2 which plays an important role in the innate immune response. This enzyme was found to exhibit bactericidal activity toward Gram-positive bacteria, but not Gram-negative ones. Though native hnpsPLA2 is active over a broad pH range, it is only highly active at alkaline conditions with the optimum activity pH of about 8.5. In order to make it highly active at neutral pH, we have obtained two hnpsPLA2 mutants, Glu89Lys and Arg100Glu that work better at neutral pH in a previous study. In the present study, we tested the bactericidal effects of the native hnpsPLA2 and the two mutants. Both native hnpsPLA2 and the two mutants exhibit bactericidal activity toward Gram-positive bacteria. Furthermore, they can also kill Escherichia coli, a Gram-negative bacterium. The two mutants showed better bactericidal activity for E. coli at neutral pH than the native enzyme, which is consistent with the enzyme activities. As hnpsPLA2 is highly stable and biocompatible, it may provide a promising therapy for bacteria infection treatment or other bactericidal applications.
authors.affiliation: BNLMS, State Key Laboratory of Structural Chemistry for Unstable and Stable Species, College of Chemistry and Molecular Engineering, Beijing 100871, China.
authors.forename: Shunchen
authors.initials: S
authors.lastname: Qiu
authors.affiliation: BNLMS, State Key Laboratory of Structural Chemistry for Unstable and Stable Species, College of Chemistry and Molecular Engineering, Beijing 100871, China; Center for Quantitative Biology, Peking University, Beijing 100871, China. Electronic address: lhlai@pku.edu.cn.
authors.forename: Luhua
authors.initials: L
authors.lastname: Lai
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects*; D054501:Group II Phospholipases A2 / Q000235:genetics / Q000494:pharmacology*; D006801:Humans; D009154:Mutation; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D011994:Recombinant Proteins; D054501:Group II Phospholipases A2
keywords: Bactericidal activity; Gram-negative bacteria; Gram-positive bacteria; Human non-pancreatic secretory phospholipase A(2); PLA(2); hnpsPLA(2); human non-pancreatic secretory phospholipase A(2); pH; phospholipase A(2); sPLA(2); secretory phospholipase A(2)