title: Conditioned media from adipose-tissue-derived mesenchymal stem cells downregulate degradative mediators induced by interleukin-1β in osteoarthritic chondrocytes.
abstract: Osteoarthritis (OA) is the most frequent joint disorder and an important cause of disability. Recent studies have shown the potential of adipose-tissue-derived mesenchymal stem cells (AD-MSC) for cartilage repair. We have investigated whether conditioned medium from AD-MSC (CM) may regulate in OA chondrocytes a number of key mediators involved in cartilage degeneration. CM enhanced type II collagen expression in OA chondrocytes while decreasing matrix metalloproteinase (MMP) activity in cell supernatants as well as the levels of MMP-3 and MMP-13 proteins and mRNA in OA chondrocytes stimulated with interleukin- (IL-) 1β. In addition, CM increased IL-10 levels and counteracted the stimulating effects of IL-1β on the production of tumor necrosis factor-α, IL-6, prostaglandin E2, and NO measured as nitrite and the mRNA expression of these cytokines, CCL-2, CCL-3, CCL-4, CCL-5, CCL-8, CCL-19, CCL-20, CXCL-1, CXCL-2, CXCL-3, CXCL-5, CXCL-8, cyclooxygenase-2, microsomal prostaglandin E synthase-1, and inducible NO synthase. These effects may be dependent on the inhibition of nuclear factor-κB activation by CM. Our data demonstrate the chondroprotective actions of CM and provide support for further studies of this approach in joint disease.
authors.affiliation: Department of Pharmacology, University of Valencia, Avenue Vicent Andrés Estellés s/n, Burjassot, 46100 Valencia, Spain.
authors.forename: Julia
authors.initials: J
authors.lastname: Platas
authors.affiliation: Department of Pharmacology, University of Valencia, Avenue Vicent Andrés Estellés s/n, Burjassot, 46100 Valencia, Spain ; Department of Pharmacy, Cardenal Herrera CEU University, Moncada, 46113 Valencia, Spain.
authors.forename: Maria Isabel
authors.initials: MI
authors.lastname: Guillén
authors.affiliation: Department of Burn and Plastic Surgery, La Fe University Hospital, 46026 Valencia, Spain.
authors.forename: María Dolores Pérez
authors.initials: MD
authors.lastname: del Caz
authors.affiliation: Department of Surgery, Faculty of Medicine, University of Valencia, 46010 Valencia, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Gomar
authors.affiliation: Valencia Transfusion Center, Generalitat Valenciana, 46014 Valencia, Spain.
authors.forename: Vicente
authors.initials: V
authors.lastname: Mirabet
authors.affiliation: Department of Pharmacology, University of Valencia, Avenue Vicent Andrés Estellés s/n, Burjassot, 46100 Valencia, Spain.
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Alcaraz
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000368:Aged; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism*; D024043:Collagen Type II / Q000032:analysis; D017077:Culture Media, Conditioned; D015536:Down-Regulation; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D053583:Interleukin-1beta / Q000494:pharmacology*; D015850:Interleukin-6 / Q000032:analysis; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000032:analysis; D019278:Matrix Metalloproteinase 3 / Q000032:analysis; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008875:Middle Aged; D016328:NF-kappa B / Q000502:physiology; D009569:Nitric Oxide / Q000096:biosynthesis; D010003:Osteoarthritis / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D024043:Collagen Type II; D017077:Culture Media, Conditioned; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D015850:Interleukin-6; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D053509:Matrix Metalloproteinase 13; D019278:Matrix Metalloproteinase 3