title: Performance of a haloalkaliphilic bioreactor under different NO3(-)/SO4(2-) ratios.
abstract: Effects of NO3(-)/SO4(2-) ratio on denitrification and sulfate removal efficiency were investigated in model experiments applying haloalkaliphilic bioreactor. The reduction of both substrates performed well at different NO3(-)/SO4(2-) ratios ranging from 17.6 to l.5. The removal rates of nitrate and sulfate were 6 and 1.39kgm(-3)d(-1), respectively, at NO3(-)/SO4(2-) ratio 3.0, while sulfide concentration reached up to 703gm(-3). The major sulfate-reducing and denitrifying bacteria were Desulfonatronovibrio sp. and Halomonas campisalis, respectively. Decrease in NO3(-)/SO4(2-) ratio led to obvious changes in bacterial community. Although the sulfate reducers became dominant, the population of denitrifying ones also increased as it was demonstrated by analysis of PCR-amplified 16S rDNA fragments, which suggested that SRB and DB coexisted well in bioreactor.
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Jie-Min
authors.initials: JM
authors.lastname: Zhou
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100190, PR China.
authors.forename: Zi-Yu
authors.initials: ZY
authors.lastname: Song
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Dao-Jiang
authors.initials: DJ
authors.lastname: Yan
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Yi-Lan
authors.initials: YL
authors.lastname: Liu
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100190, PR China.
authors.forename: Mao-Hua
authors.initials: MH
authors.lastname: Yang
authors.affiliation: National Engineering Laboratory for Hydrometallurgical Cleaner Production Technology, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100190, PR China.
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Cao
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100190, PR China. Electronic address: jmxing@home.ipe.ac.cn.
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Xing
mesh_terms: D000468:Alkalies / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D044822:Biodiversity; D019149:Bioreactors / Q000382:microbiology*; D058645:Denaturing Gradient Gel Electrophoresis; D058440:Denitrification / Q000187:drug effects; D000431:Ethanol / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000378:metabolism; D006219:Halogens / Q000494:pharmacology*; D007773:Lactates / Q000378:metabolism; D008969:Molecular Sequence Data; D009566:Nitrates / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013431:Sulfates / Q000302:isolation & purification / Q000494:pharmacology*; D013440:Sulfides / Q000494:pharmacology; D014865:Waste Disposal, Fluid
chemical_list: D000468:Alkalies; D005232:Fatty Acids, Volatile; D006219:Halogens; D007773:Lactates; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D013431:Sulfates; D013440:Sulfides; D000431:Ethanol
keywords: Denitrifying bacteria; Haloalkaliphilic; Inhibition; Sulfate-reducing bacteria