title: Production of D-arabitol from raw glycerol by Candida quercitrusa.
abstract: To promote the effective use of raw glycerol (a by-product of biodiesel production), 110 yeast strains that produce D-arabitol from glycerol were isolated from environmental samples. Among them, strain 17-2A was an effective D-arabitol producer in the presence of 250 g/l glycerol and was identified as Candida quercitrusa based on morphological, physicochemical, and phylogenetic analyses. C. quercitrusa type strain NBRC1022 produced the greatest quantity of D-arabitol (41.7 g/l) when the ability to produce D-arabitol from raw glycerol was compared among C. quercitrusa 17-2A and its phylogenetically related strains in flask culture. Under optimized culture conditions, strain NBRC1022 produced D-arabitol at a concentration of 58.2 g/l after a 7-day cultivation in 250 g/l glycerol, 6 g/l yeast extract, and 2 g/l CaCl2. The culture conditions were further investigated with raw glycerol using a jar fermenter; the concentration of D-arabitol reached 67.1 g/l after 7 days and 85.1 g/l after 10 days, respectively, which corresponded to 0.40 g/g of glycerol. To our knowledge, the present D-arabitol yield from glycerol is higher than reported previously using microbial production.
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Yoshikawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Habe
authors.forename: Tomotake
authors.initials: T
authors.lastname: Morita
authors.forename: Tokuma
authors.initials: T
authors.lastname: Fukuoka
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Imura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Iwabuchi
authors.forename: Shingo
authors.initials: S
authors.lastname: Uemura
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Tamura
authors.forename: Dai
authors.initials: D
authors.lastname: Kitamoto
mesh_terms: D001711:Biotransformation; D002175:Candida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D005990:Glycerol / Q000378:metabolism*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013402:Sugar Alcohols / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal; D013402:Sugar Alcohols; D005990:Glycerol; C014999:arabitol