title: Association between E-selectin expression and histopathological modification of glomerular lesions by non-nephritogenic IgM antibodies in experimental lupus nephritis.
abstract: OBJECTIVE
  To examine the role played by E-selectin in bystander IgM-mediated modification of glomerular lesions in experimental lupus nephritis.
  
  
  METHODS
  Experimental lupus SCID mice were induced by an intraperitoneal injection of clone 7B6.8, which was derived from a MRL/lpr mouse and shown to induce wire-loop type glomerular lesions. Mice were subsequently administered clone Sp6, a non-nephritogenic IgM antibody- producing hybridoma. E-selectin expression was then evaluated in glomeruli showing histopathological conversion of lesions from wire-loop-like to a cell-proliferative form. We also investigated the effects of a circulating soluble form of E-selectin (sE-selectin) on the modification of glomerular lesions in this lupus model.
  
  
  RESULTS
  In experimental lupus mice, glomerular E-selectin expression significantly increased during the conversion from wire-loop-like glomerular lesions to a cell-proliferative type mediated by a non-nephritogenic bystander IgM antibody in presence of a nephritogenic antibody. Intraglomerular infiltration of CD68 + macrophages correlated significantly with the glomerular level of E-selectin expression. In addition, overexpression of circulating sE-selectin significantly suppressed conversion to cell-proliferative glomerular lesions and glomerular macrophage infiltration in these lupus model mice.
  
  
  CONCLUSIONS
  The histopathological modification of lupus nephritis by non-nephritogenic bystander IgM antibodies is associated in part with glomerular E-selectin expression.
authors.affiliation: Department of Nephrology and Rheumatology, Kyoto Yamashiro General Medical Center , Kizugawa, Kyoto , Japan.
authors.forename: Kimihiko
authors.initials: K
authors.lastname: Nakatani
authors.forename: Osamu
authors.initials: O
authors.lastname: Asai
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Sakan
authors.forename: Shuhei
authors.initials: S
authors.lastname: Yoshimoto
authors.forename: Miho
authors.initials: M
authors.lastname: Terada
authors.forename: Masato
authors.initials: M
authors.lastname: Nose
authors.forename: Masayuki
authors.initials: M
authors.lastname: Iwano
authors.forename: Noboru
authors.initials: N
authors.lastname: Konishi
mesh_terms: D000818:Animals; D019040:E-Selectin / Q000378:metabolism*; D007075:Immunoglobulin M / Q000276:immunology*; D007678:Kidney Glomerulus / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008181:Lupus Nephritis / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D019463:Mice, Inbred MRL lpr; D016513:Mice, SCID
chemical_list: D019040:E-Selectin; D007075:Immunoglobulin M
keywords: E-selectin; Histopathology; IgM antibody; Lupus nephritis; Nephritogenic antibody