title: High-density polymer microarrays: identifying synthetic polymers that control human embryonic stem cell growth.
abstract: The fabrication of high-density polymer microarray is described, allowing the simultaneous and efficient evaluation of more than 7000 different polymers in a single-cellular-based screen. These high-density polymer arrays are applied in the search for synthetic substrates for hESCs culture. Up-scaling of the identified hit polymers enables long-term cellular cultivation and promoted successful stem-cell maintenance.
authors.affiliation: School of Chemistry, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh, EH9 3JJ, UK.
authors.forename: Anne
authors.initials: A
authors.lastname: Hansen
authors.forename: Heidi K
authors.initials: HK
authors.lastname: Mjoseng
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.forename: Michail
authors.initials: M
authors.lastname: Kalloudis
authors.forename: Vasileios
authors.initials: V
authors.lastname: Koutsos
authors.forename: Paul A
authors.initials: PA
authors.lastname: de Sousa
authors.forename: Mark
authors.initials: M
authors.lastname: Bradley
mesh_terms: D049109:Cell Proliferation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D016256:Lewis X Antigen / Q000235:genetics / Q000378:metabolism; D046228:Microarray Analysis; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry*; D055746:Stage-Specific Embryonic Antigens / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D016256:Lewis X Antigen; D011108:Polymers; D055746:Stage-Specific Embryonic Antigens; D014157:Transcription Factors; C066584:stage-specific embryonic antigen-4
keywords: imaging; polymer microarrays; regenerative medicine; stem cells; tissue engineering