title: Loss of MED1 triggers mitochondrial biogenesis in C2C12 cells.
abstract: Under stress conditions transcription factors, including their coactivators, play major roles in mitochondrial biogenesis and oxidative phosphorylation. MED1 (Mediator complex subunit 1) functions as a coactivator of several transcription factors and is implicated in adipogenesis of the lipid and glucose metabolism. This suggests that MED1 may play a role in mitochondrial function. In this study, we found that both the mtDNA content and mitochondrial mass were markedly increased and cell proliferation markedly suppressed in MED1-deficient cells. Upon MED1 loss, Nrf1 and its downstream target genes involved in mitochondrial biogenesis (Tfam, Plormt, Tfb1m), were up-regulated as were those genes in the OXPHOS pathway. Moreover, the knockdown of MED1 resulted in significant changes in the profile of mitochondrial respiration, accompanied by a prominent decrease in the generation of ATP. Collectively, these observations strongly suggest that MED1 has an important affect on mitochondrial function. This further elucidates the role of MED1, particularly its role in the energy metabolism.
authors.affiliation: Department of Genetics, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Jialing
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Genetics, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of Genetics, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Junxia
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Genetics, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Ji
authors.affiliation: Department of Genetics, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Genetics, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Genetics, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Xiaofen
authors.initials: X
authors.lastname: Jin
authors.affiliation: Department of Genetics, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Genetics, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Min-Xin
authors.initials: MX
authors.lastname: Guan
authors.affiliation: Department of Genetics, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China. Electronic address: ppjiang@zju.edu.cn.
authors.forename: Pingping
authors.initials: P
authors.lastname: Jiang
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D019069:Cell Respiration; D055785:Gene Knockdown Techniques; D056913:Mediator Complex Subunit 1 / Q000172:deficiency* / Q000378:metabolism*; D051379:Mice; D063269:Mitochondrial Turnover; D032446:Myoblasts / Q000502:physiology*; D051266:Nuclear Respiratory Factor 1 / Q000378:metabolism
chemical_list: C538856:Med1 protein, mouse; D056913:Mediator Complex Subunit 1; C495634:Nrf1 protein, mouse; D051266:Nuclear Respiratory Factor 1; D000255:Adenosine Triphosphate
keywords: MED1; Mitochondrial biogenesis; Nrf1; Oxygen consumption