title: OPN gene polymorphism and the serum OPN levels confer the susceptibility and prognosis of ischemic stroke in Chinese patients.
abstract: OBJECTIVE
  To investigate the association of Osteopontin (OPN) gene polymorphism and serum thrombin-cleaved OPN level with the susceptibility to ischemic stroke (IS) and its prognosis.
  
  
  METHODS
  A total of 377 patients with IS and 551 healthy individuals were recruited. The OPN gene polymorphisms at -156 G>GG, -443 C>T and -66 T>G were genotyped. Serum full-length and the thrombin-cleaved OPN were determined.
  
  
  RESULTS
  We found that only the -443 C>T polymorphism was significantly associated with the susceptibility to IS. The -443 CC represented a near 2 time higher risk for IS incidence than TT carriers. Also, the -443 CC genotype had significantly poorer outcome and they significantly had higher occurrence for bad recovery as determined by modified Rankin Scale (mRS) (OR=2.18, p=0.043) and Barthel Index (BI) (OR=2.12, p=0.05). The mean serum thrombin-cleaved OPN level in IS group were significantly higher than that in control group. ROC analysis showed that the thrombin-cleaved OPN level (cut-off value, 166.8 ng/ml) can discriminate IS patients from controls with a specificity of 86.3% and a sensitivity of 57.7%. The serum thrombin-cleaved OPN was significantly associated with the clinical outcome at 12 months after discharge from hospital.
  
  
  CONCLUSIONS
  These results suggest that the -443 C>T polymorphism of OPN gene and serum thrombin-cleaved OPN can be used as a biomarker for the susceptibility and prognosis of IS patients.
authors.affiliation: Department of Neurosurgery, the 1 Affiliated Hospital of Harbin Medical University, Harbin, Harbin, P.R. China.
authors.forename: Meng
authors.initials: M
authors.lastname: Jing
authors.forename: Baoxin
authors.initials: B
authors.lastname: Li
authors.forename: Xuwei
authors.initials: X
authors.lastname: Hou
authors.forename: Jayaram
authors.initials: J
authors.lastname: Shoba
authors.forename: Changyu
authors.initials: C
authors.lastname: Li
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Liang
authors.forename: Xiangtong
authors.initials: X
authors.lastname: Zhang
authors.forename: Enzhong
authors.initials: E
authors.lastname: Liu
authors.forename: Baofeng
authors.initials: B
authors.lastname: Yang
authors.forename: Xiangxi
authors.initials: X
authors.lastname: Meng
mesh_terms: D000328:Adult; D000483:Alleles; D019540:Area Under Curve; D044466:Asian People / Q000235:genetics*; D002681:China; D004198:Disease Susceptibility; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D053495:Osteopontin / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D012372:ROC Curve; D012720:Severity of Illness Index; D020521:Stroke / Q000175:diagnosis / Q000235:genetics*; D013917:Thrombin / Q000378:metabolism
chemical_list: D053495:Osteopontin; D013917:Thrombin