title: Differential regulation of TRPC4 in the vasopressin magnocellular system by water deprivation and hepatic cirrhosis in the rat.
abstract: Transient receptor potential canonical subtype 4 (TRPC4) is expressed in the magnocellular paraventricular nucleus (PVN) and supraoptic nucleus (SON) of the hypothalamus. In this study, the regulation of TRPC4 expression was investigated in water deprivation and hepatic cirrhosis. We used laser capture microdissection technique for precise dissection of pure AVP cell population in the PVN and SON followed by quantitative real-time RT-PCR, and immunodetection techniques by Western blot analysis and immunofluorescence. Bile duct ligation elevated TRPC4 transcripts in the SON but not PVN with correlated changes in the protein expression in these regions, as well as increased colocalization with AVP in the SON, with no changes in the PVN. Water deprivation resulted in increased TRPC4 mRNA expression in the PVN, while it decreased channel expression levels in the SON. In both of these regions, protein expression measured from tissue punches were unaltered following water deprivation, with no changes in the number of TRPC4-positive cells. Thus, TRPC4 expression is differentially regulated in physiological and pathophysiological models of vasopressin release.
authors.affiliation: Department of Integrative Physiology, and Cardiovascular Research Institute, University of North Texas Health Science Centre at Fort Worth, Fort Worth, Texas.
authors.forename: T Prashant
authors.initials: TP
authors.lastname: Nedungadi
authors.forename: J Thomas
authors.initials: JT
authors.lastname: Cunningham
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000235:genetics / Q000378:metabolism; D001652:Bile Ducts; D001798:Blood Proteins; D015153:Blotting, Western; D005786:Gene Expression Regulation / Q000502:physiology*; D007031:Hypothalamus / Q000166:cytology; D008026:Ligation; D008103:Liver Cirrhosis / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000378:metabolism; D009994:Osmolar Concentration; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D050052:TRPC Cation Channels / Q000235:genetics / Q000378:metabolism*; D014667:Vasopressins / Q000502:physiology*; D014868:Water Deprivation
chemical_list: D001798:Blood Proteins; D012333:RNA, Messenger; D050052:TRPC Cation Channels; C489571:TRPC4 ion channel; D014667:Vasopressins; D001127:Arginine Vasopressin
keywords: TRPC4; bile duct ligation; osmolality; vasopressin; water deprivation