title: Structure-function analysis of the ATP-driven glycolipid efflux pump DevBCA reveals complex organization with TolC/HgdD.
abstract: In Gram-negative bacteria, trans-envelope efflux pumps have periplasmic membrane fusion proteins (MFPs) as essential components. MFPs act as mediators between outer membrane factors (OMFs) and inner membrane factors (IMFs). In this study, structure-function relations of the ATP-driven glycolipid efflux pump DevBCA-TolC/HgdD from the cyanobacterium Anabaena sp. PCC 7120 were analyzed. The binding of the MFP DevB to the OMF TolC absolutely required the respective tip-regions. The interaction of DevB with the IMF DevAC mainly involved the β-barrel and the lipoyl domain. Efficient binding to DevAC and TolC, substrate recognition and export activity by DevAC were dependent on stable DevB hexamers.
authors.affiliation: Institute of Microbiology and Infection Medicine/Organismic Interactions, University of Tübingen, 72076 Tübingen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Staron
authors.affiliation: Institute of Microbiology and Infection Medicine/Organismic Interactions, University of Tübingen, 72076 Tübingen, Germany.
authors.forename: Karl
authors.initials: K
authors.lastname: Forchhammer
authors.affiliation: Institute of Microbiology and Infection Medicine/Organismic Interactions, University of Tübingen, 72076 Tübingen, Germany. Electronic address: iris.maldener@uni-tuebingen.de.
authors.forename: Iris
authors.initials: I
authors.lastname: Maldener
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D017033:Anabaena / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006017:Glycolipids / Q000235:genetics / Q000378:metabolism*; D050576:Membrane Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019897:Periplasm / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D013329:Structure-Activity Relationship
chemical_list: D001425:Bacterial Outer Membrane Proteins; D006017:Glycolipids; D050576:Membrane Fusion Proteins; D000255:Adenosine Triphosphate
keywords: ABC; ABC-transporter; ATP-binding cassette; Anabaena; Cyanobacteria; Heterocyst; IMF; MFP; OMF; RND; SEC; SPR; TolC; Type-I-secretion-system; inner membrane factor; membrane fusion protein; outer membrane factor; resistance-nodulation-division; size exclusion chromatography; surface plasmon resonance