title: P2X1 receptors localized in lipid rafts mediate ATP motor responses in the human vas deferens longitudinal muscles.
abstract: To assess the role of the P2X1 receptors (P2X1R) in the longitudinal and circular layers of the human vas deferens, ex vivo-isolated strips or rings were prepared from tissue biopsies to record isometric contractions. To ascertain its membrane distribution, tissue extracts were analyzed by immunoblotting following sucrose gradient ultracentrifugation. ATP, alpha,beta-methylene ATP, or electrical field stimulation elicited robust contractions of the longitudinal layer but not of the circular layer which demonstrated inconsistent responses. Alpha,beta-methylene ATP generated stronger and more robust contractions than ATP. In parallel, prostatic segments of the rat vas deferens were examined. The motor responses in both species were not sustained but decayed within the first minute, showing desensitization to additional applications. Cross-desensitization was established between alpha,beta-methylene ATP or ATP-evoked contractions and electrical field stimulation-induced contractions. Full recovery of the desensitized motor responses required more than 30 min and showed a similar pattern in human and rat tissues. Immunoblot analysis of the human vas deferens extracts revealed a P2X1R oligomer of approximately 200 kDa under nonreducing conditions, whereas dithiothreitol-treated extracts showed a single band of approximately 70 kDa. The P2X1R was identified in ultracentrifugation fractions containing 15%-29% sucrose; the receptor localized in the same fractions as flotillin-1, indicating that it regionalized into smooth muscle lipid rafts. In conclusion, ATP plays a key role in human vas deferens contractile responses of the longitudinal smooth muscle layer, an effect mediated through P2X1Rs.
authors.affiliation: Laboratorio de Nucleótidos, Departamento de Fisiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: María Verónica
authors.initials: MV
authors.lastname: Donoso
authors.forename: Andrés
authors.initials: A
authors.lastname: Norambuena
authors.forename: Camilo
authors.initials: C
authors.lastname: Navarrete
authors.forename: Inés
authors.initials: I
authors.lastname: Poblete
authors.forename: Alfredo
authors.initials: A
authors.lastname: Velasco
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Huidobro-Toro
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000328:Adult; D000368:Aged; D000818:Animals; D004558:Electric Stimulation; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D021962:Membrane Microdomains / Q000378:metabolism*; D008875:Middle Aged; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D021381:Protein Transport; D051381:Rats; D017207:Rats, Sprague-Dawley; D058474:Receptors, Purinergic P2X1 / Q000378:metabolism / Q000502:physiology*; D014649:Vas Deferens / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*
chemical_list: D058474:Receptors, Purinergic P2X1; D000255:Adenosine Triphosphate
keywords: ATP-induced contractility; P2X receptor desensitization; P2X1 receptors; P2X1 receptors in lipid rafts; human vas deferens; sympathetic cotransmission