title: Identification of a mechanism for lung inflammation caused by Mycoplasma pneumoniae using a novel mouse model.
abstract: Human Mycoplasma pneumoniae (MP) pneumonia is characterized by alveolar infiltration with neutrophils and lymphocytes and lymphocyte/plasma cell infiltrates in the peri-bronchovascular area (PBVA). No mouse model has been able to mimic the pathological features seen in human MP pneumonia, such as plasma cell-rich lymphocytic infiltration in PBVA. To figure out the mechanism for inflammation by MP infection using a novel mouse model that mimics human MP pneumonia, mice were pre-immunized intraperitoneally with Th2 stimulating adjuvant, alum, alone or MP extracts with an alum, followed by intratracheal challenge with MP extracts. The toll-like receptor-2, which is the major receptor for mycoplasma cell wall lipoproteins, was strongly up-regulated in alveolar macrophages in a latter group after the pre-immunization but prior to the intratracheal challenge. Those findings demonstrated that acceleration of innate immunity by antecedent antigenic stimulation can be an important positive-feedback mechanism in lung inflammation during MP pneumonia.
authors.affiliation: Department of Respiratory Medicine, Kyorin University School of Medicine, 6-20-2, Shinkawa, Mitaka City, Tokyo, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Saraya
authors.affiliation: Niigata University Medical & Dental Hospital, Bioscience Medical Research Center, 1-754, Asashimachi-dori, Chuo-ku, Niigata 951-8520, Japan.
authors.forename: Koh
authors.initials: K
authors.lastname: Nakata
authors.affiliation: Laboratory of Infectious Diseases and Immunology, College of Veterinary Medicine, Nippon Jui Seimei-kagaku University, Musashino, Tokyo 180-8602, Japan.
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Nakagaki
authors.affiliation: Niigata University Medical & Dental Hospital, Bioscience Medical Research Center, 1-754, Asashimachi-dori, Chuo-ku, Niigata 951-8520, Japan.
authors.forename: Natsuki
authors.initials: N
authors.lastname: Motoi
authors.affiliation: NTT Medical Center Tokyo, Department of Diagnostic Pathology, Japan.
authors.forename: Kuniko
authors.initials: K
authors.lastname: Iihara
authors.affiliation: Department of Pathology, Kyorin University School of Medicine, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Fujioka
authors.affiliation: Division of Pathology and Central Clinical Laboratory, Kanto Central Hospital of the Mutual Aid Association of Public School Teachers, Japan.
authors.forename: Teruaki
authors.initials: T
authors.lastname: Oka
authors.affiliation: Department of Respiratory Medicine, Kyorin University School of Medicine, 6-20-2, Shinkawa, Mitaka City, Tokyo, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kurai
authors.affiliation: Department of Respiratory Medicine, Kyorin University School of Medicine, 6-20-2, Shinkawa, Mitaka City, Tokyo, Japan.
authors.forename: Hiroo
authors.initials: H
authors.lastname: Wada
authors.affiliation: Department of Respiratory Medicine, Kyorin University School of Medicine, 6-20-2, Shinkawa, Mitaka City, Tokyo, Japan.
authors.forename: Haruyuki
authors.initials: H
authors.lastname: Ishii
authors.affiliation: Department of Immunology, Kyorin University, Faculty of Health Sciences, Japan.
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Taguchi
authors.affiliation: Department of Infectious Diseases, Kyorin University School of Medicine, 6-20-2, Shinkawa, Mitaka City, Tokyo, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kamiya
authors.affiliation: Department of Respiratory Medicine, Kyorin University School of Medicine, 6-20-2, Shinkawa, Mitaka City, Tokyo, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Goto
keywords: AMs, alveolar macrophage; Alveolar macrophage; BAL, bronchoalveolar lavage; IT, intratracheal challenge; MP pneumonia, Mycoplasma pneumonia; MP, Mycoplasma pneumoniae; Mice model; Mycoplasma pneumonia extracts; Mycoplasma pneumoniae pneumonia; PBA, peribronchiolare area; PBVA, peri-bronchovascular area; PVA, perivascular area; Plasma cell; TLR, Toll-like receptor; Toll-like receptor-2