title: Galactosylation of human erythropoietin produced by chimeric chickens expressing galactosyltransferase.
abstract: Human erythropoietin produced in the egg white of chimeric chicken contains N-glycan with lower amounts of terminal galactose and sialic acid; therefore, the chicken galactosyltransferase gene was introduced together with the human erythropoietin gene by a retroviral vector. We found that erythropoietin accumulated in the egg white was partially galactosylated.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kojima
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Wakita
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Yujin
authors.initials: Y
authors.lastname: Inayoshi
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Suzuki
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yamada
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan. Electronic address: kaneoka@nubio.nagoya-u.ac.jp.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Kaneoka
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Nishijima
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Iijima
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics; D030161:Avian Proteins / Q000096:biosynthesis / Q000235:genetics; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D004527:Egg Proteins / Q000378:metabolism; D004921:Erythropoietin / Q000235:genetics / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism*; D005700:Galactosyltransferases / Q000096:biosynthesis / Q000235:genetics; D006031:Glycosylation; D006801:Humans; D010957:Plasmids; D011499:Protein Processing, Post-Translational; D018116:Vesiculovirus / Q000235:genetics
chemical_list: D030161:Avian Proteins; C554270:EPO protein, human; D004527:Egg Proteins; D004921:Erythropoietin; D005700:Galactosyltransferases; D005690:Galactose
keywords: Erythropoietin; Galactose; Galactosyltransferase; N-Glycan; Transgenic chicken