title: Metabolomics reveals metabolite changes in acute pulmonary embolism.
abstract: Pulmonary embolism (PE) is a common cardiovascular emergency which can lead to pulmonary hypertension (PH) and right ventricular failure as a consequence of pulmonary arterial bed occlusion. The diagnosis of PE is challenging due to nonspecific clinical presentation, which results in relatively high mortality. Moreover, the pathological factors associated with PE are poorly understood. Metabolomics can provide new highlights which can help in the understanding of the processes and even propose biomarkers for its diagnosis. In order to obtain more information about PE and PH, acute PE was induced in large white pigs and plasma was obtained before and after induction of PE. Metabolic fingerprints from plasma were obtained with LC-QTOF-MS (positive and negative ionization) and GC-Q-MS. Data pretreatment and statistical analysis (uni- and multivariate) were performed in order to compare metabolic fingerprints and to select the metabolites that showed higher loading for the classification (28 from LC and 19 from GC). The metabolites found differentially distributed among groups are mainly related to energy imbalance in hypoxic conditions, such as glycolysis-derived metabolites, ketone bodies, and TCA cycle intermediates, as well as a group of lipidic mediators that could be involved in the transduction of the signals to the cells such as sphingolipids and lysophospholipids, among others. Results presented in this report reveal that combination of LC-MS- and GC-MS-based metabolomics could be a powerful tool for diagnosis and understanding pathophysiological processes due to acute PE.
authors.affiliation: Centre for Metabolomics and Bioanalysis (CEMBIO), Facultad de Farmacia, Universidad CEU San Pablo , Campus Monteprincipe, Boadilla del Monte 28668, Madrid, Spain.
authors.forename: Renata
authors.initials: R
authors.lastname: Bujak
authors.forename: Ana
authors.initials: A
authors.lastname: García-Álvarez
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Rupérez
authors.forename: Mario
authors.initials: M
authors.lastname: Nuño-Ayala
authors.forename: Antonia
authors.initials: A
authors.lastname: García
authors.forename: Jesus
authors.initials: J
authors.lastname: Ruiz-Cabello
authors.forename: Valentín
authors.initials: V
authors.lastname: Fuster
authors.forename: Borja
authors.initials: B
authors.lastname: Ibáñez
authors.forename: Coral
authors.initials: C
authors.lastname: Barbas
mesh_terms: D000208:Acute Disease; D000818:Animals; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D055432:Metabolomics; D011655:Pulmonary Embolism / Q000378:metabolism*; D013552:Swine