title: A new diphenyl ether from the endophytic fungus Verticillium sp. isolated from Rehmannia glutinosa.
abstract: OBJECTIVE
  To investigate the chemical constituents of the endophytic fungus Verticillium sp. isolated from Rehmannia glutinosa.
  
  
  METHODS
  The compounds were isolated and purified by repeated column chromatography, and their structures were determined on the basis of physicochemical properties and spectral analysis. Their cytotoxic and antifungal activities were evaluated.
  
  
  RESULTS
  Ten compounds were obtained and their structures were identified as 2, 4-dihydroxy-2', 6-diacetoxy-3'-methoxy-5'-methyl-diphenyl ether (1), paecilospirone (2), α-acetylorcinol (3), 2-methoxy-1,8-dimethyl-xanthen-9-one (4), 4-hydroxy-α-lapachone (5), enalin A (6), 2,3,4-trimethyl-5,7-dihydroxy-2,3-dihydrobenzofuran (7), 4-hydroxyethyl-phenol (8), 2,4-dihydroxy-3,5,6-trimethyl- methylbenzoate (9), and 3-isopropenyl-(Z)-monomethyl maleate (10).
  
  
  CONCLUSIONS
  Compound 1 is a new diphenyl ether, and showed cytotoxic activity against HL-60 cells (IC50 2.24 μg · mL(-1)), and antifungal activities against Candida albicans (MIC 8 μg · mL(-1)) and Aspergillus fumigatus (MIC 16 μg · mL(-1)).
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China; Department of Pharmacology, College of Pharmacy, Third Military Medical University, Chongqing 400038, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Peng
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Fei
authors.initials: F
authors.lastname: You
authors.affiliation: Department of Pharmacology, College of Pharmacy, Third Military Medical University, Chongqing 400038, China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Li
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Min
authors.initials: M
authors.lastname: Jia
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Cheng-Jian
authors.initials: CJ
authors.lastname: Zheng
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China. Electronic address: hanting@smmu.edu.cn.
authors.forename: Ting
authors.initials: T
authors.lastname: Han
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai 200433, China. Electronic address: qinsmmu@126.com.
authors.forename: Lu-Ping
authors.initials: LP
authors.lastname: Qin
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000493:pharmacokinetics; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000493:pharmacokinetics; D001232:Aspergillus fumigatus / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D045744:Cell Line, Tumor; D060026:Endophytes / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D010647:Phenyl Ethers / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000493:pharmacokinetics; D032267:Rehmannia / Q000382:microbiology*; D020172:Verticillium / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D000970:Antineoplastic Agents; D010647:Phenyl Ethers; C031538:phenyl ether
keywords: Antifungal activities; Cytotoxic activity; Diphenyl ether; Endophytic fungus; Rehmannia glutinosa