title: Combination of culture-dependent and -independent methods reveals diverse acyl homoserine lactone-producers from rhizosphere of wetland plants.
abstract: The culture-dependent method and a degenerate primer-based culture-independent method were combined in an effort to identify N-acyl homoserine lactone (AHL) producers in rhizosphere of wetland plants, Salix babylonica (willow) and Phragmites australis (reed). Overall, eight potential AHL-producing genera were found, which were Aeromonas, Pseudomonas, Polymorphum, Agrobacterium, Rhizobium, Sinorhizobium, Ensifer, and Pectobacterium. Thin layer chromatograph assay revealed various AHL profiles from cultivable AHL-producers. The degenerate primer pair RAHL352F and RAHL461R was found to cover AHL synthetase genes from families Rhizobiaceae and Rhodobacteraceae. Little overlap was found in taxa of potential AHL-producers obtained by the two methods, indicating that they were well complement to each other. This is the first survey for AHL-producers that employed combined culture-dependent and -independent methods.
authors.affiliation: Department of Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Room B470, 688 Yu Hang Tang Road, Hangzhou, 310058, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Zeng
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Yu
authors.forename: Yili
authors.initials: Y
authors.lastname: Huang
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism*; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002855:Chromatography, Thin Layer; D008025:Ligases / Q000235:genetics; D008969:Molecular Sequence Data; D006109:Poaceae / Q000382:microbiology*; D058441:Rhizosphere; D032108:Salix / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D053833:Wetlands
chemical_list: D054742:Acyl-Butyrolactones; D008025:Ligases; C449816:N-acylhomoserine lactone synthase