title: Role of Acinetobacter baumannii UmuD homologs in antibiotic resistance acquired through DNA damage-induced mutagenesis.
abstract: The role of Acinetobacter baumannii ATCC 17978 UmuDC homologs A1S_0636-A1S_0637, A1S_1174-A1S_1173, and A1S_1389 (UmuDAb) in antibiotic resistance acquired through UV-induced mutagenesis was evaluated. Neither the growth rate nor the UV-related survival of any of the three mutants was significantly different from that of the wild-type parental strain. However, all mutants, and especially the umuDAb mutant, were less able to acquire resistance to rifampin and streptomycin through the activities of their error-prone DNA polymerases. Furthermore, in the A. baumannii mutant defective in the umuDAb gene, the spectrum of mutations included a dramatic reduction in the frequency of transition mutations, the mutagenic signature of the DNA polymerase V encoded by umuDC.
authors.affiliation: Departament de Genètica i Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona (UAB), Barcelona, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Aranda
authors.forename: Mario
authors.initials: M
authors.lastname: López
authors.forename: Enoy
authors.initials: E
authors.lastname: Leiva
authors.forename: Andrés
authors.initials: A
authors.lastname: Magán
authors.forename: Ben
authors.initials: B
authors.lastname: Adler
authors.forename: Germán
authors.initials: G
authors.lastname: Bou
authors.forename: Jordi
authors.initials: J
authors.lastname: Barbé
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004249:DNA Damage / Q000235:genetics* / Q000528:radiation effects; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D016296:Mutagenesis / Q000235:genetics; D012293:Rifampin / Q000494:pharmacology; D013307:Streptomycin / Q000494:pharmacology; D014466:Ultraviolet Rays
chemical_list: D000900:Anti-Bacterial Agents; D004259:DNA-Directed DNA Polymerase; D012293:Rifampin; D013307:Streptomycin