title: Therapeutic effect of Halofuginone on ITP mice by regulating the differentiation of Th cell subsets.
abstract: The aim of the present study was to investigate the therapeutic effect of halofuginone (HF) in the treatment of idiopathic thrombocytopenic purpura (ITP) and explore the underlying mechanism. Sixty ITP mice were divided into four groups including control group, low dose group (25 mg/kg HF), medium dose group (50 mg/kg HF), and high dose group (100 mg/kg HF). Corresponding dose of HF was administrated by gavage daily in HF groups for 7 days, and the same volume of saline was given in control group. Platelet counts were 28.87 ± 3.91 × 10(9)/L, 57.13 ± 2.75 × 10(9)/L, 86.73 ± 3.06 × 10(9)/L and 89.73 ± 2.84 × 10(9)/L in control group, low dose group, medium dose group, and high dose group respectively, on day 7 after intragastrically administration of HF or saline. Compared with control group, three HF groups showed significantly increased levels of INF-γ and IL-2 (all P < 0.05), and significantly decreased concentrations of IL-4 and IL-10(all P < 0.05). The expression of T-bet mRNA increased and the expression of GATA-3 mRNA decreased (all P < 0.05) in ITP mice after intragastric administration with different dose of HF. HF significantly recovered peripheral platelet counts in ITP mice through promoting Th1 cell differentiation and attenuating Th2 differentiation in ITP mice.
authors.affiliation: Analytical Section of Attached Hospital of Jining Medical College, Jining, Shandong Province, PR China.
authors.forename: Chengqiang
authors.initials: C
authors.lastname: Jin
authors.affiliation: Radiology Department of Jining First People's Hospital, Jining, Shandong Province, PR China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Analytical Section of Attached Hospital of Jining Medical College, Jining, Shandong Province, PR China.
authors.forename: Chengying
authors.initials: C
authors.lastname: Jin
authors.affiliation: Analytical Section of Attached Hospital of Jining Medical College, Jining, Shandong Province, PR China.
authors.forename: Haixin
authors.initials: H
authors.lastname: Dong
authors.affiliation: Analytical Section of Attached Hospital of Jining Medical College, Jining, Shandong Province, PR China.
authors.forename: Panpan
authors.initials: P
authors.lastname: Cheng
authors.affiliation: Analytical Section of Attached Hospital of Jining Medical College, Jining, Shandong Province, PR China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Pediatric Emergency Department of Attached Hospital of Jining Medical College, Jining, Shandong Province, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Immunology and Microbiology, Guangdong Medical College, Dongguan, Guangdong Province, PR China.
authors.forename: Biying
authors.initials: B
authors.lastname: Zheng
authors.affiliation: Department of Immunology and Microbiology, Guangdong Medical College, Dongguan, Guangdong Province, PR China. Electronic address: jincq2008@163.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
mesh_terms: D000818:Animals; D017252:CD3 Complex / Q000276:immunology; D002454:Cell Differentiation; D016207:Cytokines / Q000097:blood; D005260:Female; D050990:GATA3 Transcription Factor / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010880:Piperidines / Q000494:pharmacology / Q000627:therapeutic use*; D010976:Platelet Count; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000097:blood / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D052999:Quinazolinones / Q000494:pharmacology / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000378:metabolism; D020825:T-Box Domain Proteins / Q000235:genetics; D018417:Th1 Cells / Q000166:cytology / Q000187:drug effects; D018418:Th2 Cells / Q000166:cytology / Q000187:drug effects; D000098815:T-bet Transcription Factor
chemical_list: D017252:CD3 Complex; D016207:Cytokines; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D010880:Piperidines; D052999:Quinazolinones; D012333:RNA, Messenger; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; C010176:halofuginone
keywords: Animal experiment; Halofuginone; Idiopathic thrombocytopenic purpura; Platelet; T cell differentiation