title: Ultrasensitive IL-6 electrochemical immunosensor based on Au nanoparticles-graphene-silica biointerface.
abstract: An Interleukin-6 (IL-6) electrochemical immunosensor was fabricated based on the Au nanoparticles (AuNP)-graphene-silica sol-gel as immobilization biointerface and AuNP-polydopamine (PDA)@carbon nanotubes (CNT) as the label of HRP-bound antibodies. The AuNP-graphene-silica sol-gel film was prepared in situ and modified on the ITO electrode, providing a stable network for the immobilization of antibody and exhibiting a dynamic working range of 1-40 pg/mL with a low detection limit of 0.3 pg/mL IL-6 (at 3s). The results of serum samples with the sensor received an acceptable agreement with the ELISA method. Importantly, this method provided a promising ultrasensitive assay strategy for clinical applications.
authors.affiliation: College of Chemistry and Materials Science, Key Laboratory for Functional Molecular Solids of the Education Ministry of China, Anhui Key Laboratory of Chem-Biosensing, Center for Nano Science and Technology, Anhui Normal University, Wuhu 241000, PR China; Anhui Key Laboratory of Controllable Chemistry Reaction & Material Chemical Engineering, HeFei University of Technology, Hefei 230009, PR China.
authors.forename: Guangfeng
authors.initials: G
authors.lastname: Wang
authors.affiliation: College of Chemistry and Materials Science, Key Laboratory for Functional Molecular Solids of the Education Ministry of China, Anhui Key Laboratory of Chem-Biosensing, Center for Nano Science and Technology, Anhui Normal University, Wuhu 241000, PR China.
authors.forename: Xiuping
authors.initials: X
authors.lastname: He
authors.affiliation: College of Chemistry and Materials Science, Key Laboratory for Functional Molecular Solids of the Education Ministry of China, Anhui Key Laboratory of Chem-Biosensing, Center for Nano Science and Technology, Anhui Normal University, Wuhu 241000, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Chemistry and Materials Science, Key Laboratory for Functional Molecular Solids of the Education Ministry of China, Anhui Key Laboratory of Chem-Biosensing, Center for Nano Science and Technology, Anhui Normal University, Wuhu 241000, PR China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: College of Chemistry and Materials Science, Key Laboratory for Functional Molecular Solids of the Education Ministry of China, Anhui Key Laboratory of Chem-Biosensing, Center for Nano Science and Technology, Anhui Normal University, Wuhu 241000, PR China. Electronic address: xjzhang@mail.ahnu.edu.cn.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zhang
mesh_terms: D055664:Electrochemical Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D007118:Immunoassay; D015850:Interleukin-6 / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D015850:Interleukin-6; D006046:Gold; D012822:Silicon Dioxide; D006108:Graphite
keywords: Electrochemical immunosensor; Graphene; Interleukin-6; Polydopamine