title: Radiation sterilized bone response to dynamic loading.
abstract: Allogeneic bone grafts are used on a large scale in surgeries. To avoid the risk of infectious diseases, allografts should be radiation-sterilized. So far, no international consensus has been achieved regarding the optimal radiation dose. Many authors suggest that bone sterilization deteriorates bone mechanical properties. However, no data on the influence of ionizing radiation on bone dynamic mechanical properties are available. Bovine femurs from 2-year old animal were machine cut and irradiated with the doses 10, 15, 25, 35, 45 and 50 kGy. Dynamic mechanical analysis was performed at 1-10 Hz at the temperature range of 0-350 °C in 3-point bending configuration. No statistically significant differences in storage modulus were observed. However, there were significant decreased values of loss modulus between the samples irradiated with doses of 10 (↓14.3%), 15, 45 and 50 kGy (↓33.2%) and controls. It was stated that increased irradiation dose decreases the temperature where collagen denaturation process starts and increases the temperature where the collagen denaturation process finishes. It was shown that activation energy of denaturation process is significantly higher for the samples irradiated with the dose of 50 kGy (615 kJ/mol) in comparison with control samples and irradiation with other doses (100-135 kJ/mol).
authors.affiliation: Department of Oncology, Poznan University of Medical Sciences, ul. Szamarzewskiego 82/84, 60-569 Poznan, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Mardas
authors.affiliation: Department of Biophysics, Poznan University of Medical Sciences, ul. Fredry 10, 61-701 Poznan, Poland.
authors.forename: Leszek
authors.initials: L
authors.lastname: Kubisz
authors.affiliation: Department of Physics, Adam Mickiewicz University, ul. Umultowska 85, 61-614 Poznan, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Biskupski
authors.affiliation: Department of Physics, Adam Mickiewicz University, ul. Umultowska 85, 61-614 Poznan, Poland.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Mielcarek
authors.affiliation: Department of Bromatology, Poznan University of Medical Sciences, ul. Marcelińska 420, 60-354 Poznan, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Stelmach-Mardas
authors.affiliation: Centre for Radiation Research and Technology, Institute of Nuclear Chemistry and Technology, ul. Dorodna 16, 03-195 Warsaw, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Kałuska
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena / Q000502:physiology / Q000528:radiation effects; D016025:Bone Transplantation / Q000379:methods*; D001842:Bone and Bones / Q000502:physiology* / Q000528:radiation effects*; D002417:Cattle; D005269:Femur / Q000502:physiology / Q000528:radiation effects; D011827:Radiation; D011829:Radiation Dosage; D013242:Sterilization / Q000379:methods