title: Functional recoveries of sciatic nerve regeneration by combining chitosan-coated conduit and neurosphere cells induced from adipose-derived stem cells.
abstract: Suboptimal repair occurs in a peripheral nerve gap, which can be partially restored by bridging the gap with various biosynthetic conduits or cell-based therapy. In this study, we developed a combination of chitosan coating approach to induce neurosphere cells from human adipose-derived stem cells (ASCs) on chitosan-coated plate and then applied these cells to the interior of a chitosan-coated silicone tube to bridge a 10-mm gap in a rat sciatic nerve. Myelin sheath degeneration and glial scar formation were discovered in the nerve bridged by the silicone conduit. By using a single treatment of chitosan-coated conduit or neurosphere cell therapy, the nerve gap was partially recovered after 6 weeks of surgery. Substantial improvements in nerve regeneration were achieved by combining neurosphere cells and chitosan-coated conduit based on the increase of myelinated axons density and myelin thickness, gastrocnemius muscle weight and muscle fiber diameter, and step and stride lengths from gait analysis. High expressions of interleukin-1β and leukotriene B4 receptor 1 in the intra-neural scarring caused by using silicone conduits revealed that the inflammatory mechanism can be inhibited when the conduit is coated with chitosan. This study demonstrated that the chitosan-coated surface performs multiple functions that can be used to induce neurosphere cells from ASCs and to facilitate nerve regeneration in combination with a cells-assisted coated conduit.
authors.affiliation: Division of Plastic Surgery, Department of Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, No. 1, University Rd., Tainan 701, Taiwan; Institute of Clinical Medicine, National Cheng Kung University, No. 1, University Rd., Tainan 701, Taiwan.
authors.forename: Yuan-Yu
authors.initials: YY
authors.lastname: Hsueh
authors.affiliation: Department of Cell Biology and Anatomy, National Cheng Kung University, No. 1, University Rd., Tainan 701, Taiwan; Institute of Basic Medical Sciences, National Cheng Kung University, No. 1, University Rd., Tainan 701, Taiwan.
authors.forename: Ya-Ju
authors.initials: YJ
authors.lastname: Chang
authors.affiliation: Department of Occupational Therapy, National Cheng Kung University, No. 1, University Rd., Tainan 701, Taiwan.
authors.forename: Tzu-Chieh
authors.initials: TC
authors.lastname: Huang
authors.affiliation: Department of Occupational Therapy, I-Shou University, No.1, Sec.1, Syuecheng Rd., Dashu District, Kaouhsiung City 84001, Taiwan.
authors.forename: Shih-Chen
authors.initials: SC
authors.lastname: Fan
authors.affiliation: Division of Plastic Surgery, Department of Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, No. 1, University Rd., Tainan 701, Taiwan.
authors.forename: Duo-Hsiang
authors.initials: DH
authors.lastname: Wang
authors.affiliation: Department of Biomedical Engineering, National Cheng Kung University, No. 1, University Rd., Tainan 701, Taiwan; Medical Device Innovation Center, National Cheng Kung University, No. 1, University Rd., Tainan 701, Taiwan.
authors.forename: Jia-Jin Jason
authors.initials: JJ
authors.lastname: Chen
authors.affiliation: Department of Cell Biology and Anatomy, National Cheng Kung University, No. 1, University Rd., Tainan 701, Taiwan; Institute of Basic Medical Sciences, National Cheng Kung University, No. 1, University Rd., Tainan 701, Taiwan; Department of Biomedical Engineering, National Cheng Kung University, No. 1, University Rd., Tainan 701, Taiwan; Medical Device Innovation Center, National Cheng Kung University, No. 1, University Rd., Tainan 701, Taiwan. Electronic address: joshccwu@mail.ncku.edu.tw.
authors.forename: Chia-Ching
authors.initials: CC
authors.lastname: Wu
authors.affiliation: Division of Plastic Surgery, Department of Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, No. 1, University Rd., Tainan 701, Taiwan. Electronic address: fredlin@tncghb.gov.tw.
authors.forename: Sheng-Che
authors.initials: SC
authors.lastname: Lin
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002478:Cells, Cultured; D048271:Chitosan; D005684:Gait; D009416:Nerve Regeneration; D051381:Rats; D012584:Sciatic Nerve / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology*
chemical_list: D048271:Chitosan
keywords: Adipose-derived stem cell; Chitosan; Nerve conduit; Neurosphere; Sciatic nerve injury; Spheroid