title: Anti-thrombogenic properties of a nitric oxide-releasing dextran derivative: evaluation of platelet activation and whole blood clotting kinetics.
abstract: Controlling platelet activation and clotting initiated by cardiovascular interventions remains a major challenge in clinical practice. In this work, the anti-thrombotic properties of a polysaccharide-based nitric oxide (NO)-releasing dextran derivative are presented. Total platelet adhesion, platelet morphology and whole blood clotting kinetics were used as indicators to evaluate the anti-clotting properties of this material. With a total NO delivery of 0.203±0.003 μmol, the NO-releasing dextran derivative (Dex-SNO) mixed with blood plasma demonstrated a significantly lower amount of platelet adhesion and activation onto a surface and reduced whole blood clotting kinetics. Nearly 75% reduction in platelet adhesion and a significant retention of platelet morphology were observed with blood plasma treated with Dex-SNO, suggesting this to be a potential anti-platelet therapeutic agent for preventing thrombosis that does not have an adverse effect on circulating platelets.
authors.affiliation: Department of Chemistry, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: Vinod B
authors.initials: VB
authors.lastname: Damodaran
authors.affiliation: Department of Mechanical Engineering and School of Biomedical Engineering, Colorado State University, Fort Collins, CO-80523, USA.
authors.forename: Victoria
authors.initials: V
authors.lastname: Leszczak
authors.affiliation: School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: Kathryn A
authors.initials: KA
authors.lastname: Wold
authors.affiliation: Department of Chemistry, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Lantvit
authors.affiliation: Department of Mechanical Engineering and School of Biomedical Engineering, Colorado State University, Fort Collins, CO-80523, USA.
authors.forename: Ketul C
authors.initials: KC
authors.lastname: Popat
authors.affiliation: Department of Chemistry, Colorado State University, Fort Collins, CO 80523, USA ; School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Reynolds
keywords: Nitric oxide; S-nitrosothiol; anti-thrombogenic; platelet activation; platelet adhesion; platelet morphology; whole blood clotting kinetics