title: Coffee husk composting: an investigation of the process using molecular and non-molecular tools.
abstract: Various parameters were measured during a 90-day composting process of coffee husk with cow dung (Pile 1), with fruit/vegetable wastes (Pile 2) and coffee husk alone (Pile 3). Samples were collected on days 0, 32 and 90 for chemical and microbiological analyses. C/N ratios of Piles 1 and 2 decreased significantly over the 90 days. The highest bacterial counts at the start of the process and highest actinobacterial counts at the end of the process (Piles 1 and 2) indicated microbial succession with concomitant production of compost relevant enzymes. Denaturing gradient gel electrophoresis of rDNA and COMPOCHIP microarray analysis indicated distinctive community shifts during the composting process, with day 0 samples clustering separately from the 32 and 90-day samples. This study, using a multi-parameter approach, has revealed differences in quality and species diversity of the three composts.
authors.affiliation: Department of Microbial, Cellular and Molecular Biology, Addis Ababa University, Ethiopia. Electronic address: fshemekite@gmail.com.
authors.forename: Fekadu
authors.initials: F
authors.lastname: Shemekite
authors.affiliation: Institute of Microbiology, University of Innsbruck, Technikerstraβe 25d, A-6020 Innsbruck, Austria.
authors.forename: María
authors.initials: M
authors.lastname: Gómez-Brandón
authors.affiliation: Institute of Microbiology, University of Innsbruck, Technikerstraβe 25d, A-6020 Innsbruck, Austria.
authors.forename: Ingrid H
authors.initials: IH
authors.lastname: Franke-Whittle
authors.affiliation: Institute of Microbiology, University of Innsbruck, Technikerstraβe 25d, A-6020 Innsbruck, Austria.
authors.forename: Barbara
authors.initials: B
authors.lastname: Praehauser
authors.affiliation: Institute of Microbiology, University of Innsbruck, Technikerstraβe 25d, A-6020 Innsbruck, Austria.
authors.forename: Heribert
authors.initials: H
authors.lastname: Insam
authors.affiliation: Department of Microbial, Cellular and Molecular Biology, Addis Ababa University, Ethiopia.
authors.forename: Fassil
authors.initials: F
authors.lastname: Assefa
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics; D002244:Carbon / Q000378:metabolism*; D002417:Cattle; D003069:Coffee; D004269:DNA, Bacterial / Q000032:analysis; D005638:Fruit / Q000737:chemistry; D005658:Fungi / Q000235:genetics; D008372:Manure / Q000032:analysis* / Q000382:microbiology; D064307:Microbiota / Q000235:genetics / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D012037:Refuse Disposal; D012988:Soil Microbiology; D013997:Time Factors; D014675:Vegetables / Q000737:chemistry
chemical_list: D003069:Coffee; D004269:DNA, Bacterial; D008372:Manure; D002244:Carbon
keywords: COMPOCHIP microarray; Coffee husk compost; Enzyme activity; Organic matter degradation; PCR-DGGE