title: Bioceramic-collagen scaffolds loaded with human adipose-tissue derived stem cells for bone tissue engineering.
abstract: The combination of bioceramics and stem cells has attracted the interest of research community for bone tissue engineering applications. In the present study, a combination of Bio-Oss(®) and type 1 collagen gel as scaffold were loaded with human adipose-tissue derived mesenchymal stem cells (AT-MSCs) after isolation and characterization, and the capacity of them for bone regeneration was investigated in rat critical size defects using digital mammography, multi-slice spiral computed tomography imaging and histological analysis. 8 weeks after implantation, no mortality or sign of inflammation was observed in the site of defect. According to the results of imaging analysis, a higher level of bone regeneration was observed in the rats receiving Bio-Oss(®)-Gel compared to untreated group. In addition, MSC-seeded Bio-Oss-Gel induced the highest bone reconstruction among all groups. Histological staining confirmed these findings and impressive osseointegration was observed in MSC-seeded Bio-Oss-Gel compared with Bio-Oss-Gel. On the whole, it was demonstrated that combination of AT-MSCs, Bio-Oss and Gel synergistically enhanced bone regeneration and reconstruction and also could serve as an appropriate structure to bone regenerative medicine and tissue engineering application.
authors.affiliation: Departments of Anatomy and Cell Biology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Neda
authors.initials: N
authors.lastname: Daei-Farshbaf
authors.forename: Abdolreza
authors.initials: A
authors.lastname: Ardeshirylajimi
authors.forename: Ehsan
authors.initials: E
authors.lastname: Seyedjafari
authors.forename: Abbas
authors.initials: A
authors.lastname: Piryaei
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Fadaei Fathabady
authors.forename: Mehdi
authors.initials: M
authors.lastname: Hedayati
authors.forename: Mohammad
authors.initials: M
authors.lastname: Salehi
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.forename: Hamid
authors.initials: H
authors.lastname: Nazarian
authors.forename: Sadegh-Lotfalah
authors.initials: SL
authors.lastname: Moradi
authors.forename: Mohsen
authors.initials: M
authors.lastname: Norouzian
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001861:Bone Regeneration; D002454:Cell Differentiation; D024042:Collagen Type I / Q000737:chemistry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008903:Minerals / Q000737:chemistry / Q000627:therapeutic use; D051381:Rats; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: C077540:Bio-Oss; D024042:Collagen Type I; D008903:Minerals