title: Benchmarking organic micropollutants in wastewater, recycled water and drinking water with in vitro bioassays.
abstract: Thousands of organic micropollutants and their transformation products occur in water. Although often present at low concentrations, individual compounds contribute to mixture effects. Cell-based bioassays that target health-relevant biological endpoints may therefore complement chemical analysis for water quality assessment. The objective of this study was to evaluate cell-based bioassays for their suitability to benchmark water quality and to assess efficacy of water treatment processes. The selected bioassays cover relevant steps in the toxicity pathways including induction of xenobiotic metabolism, specific and reactive modes of toxic action, activation of adaptive stress response pathways and system responses. Twenty laboratories applied 103 unique in vitro bioassays to a common set of 10 water samples collected in Australia, including wastewater treatment plant effluent, two types of recycled water (reverse osmosis and ozonation/activated carbon filtration), stormwater, surface water, and drinking water. Sixty-five bioassays (63%) showed positive results in at least one sample, typically in wastewater treatment plant effluent, and only five (5%) were positive in the control (ultrapure water). Each water type had a characteristic bioanalytical profile with particular groups of toxicity pathways either consistently responsive or not responsive across test systems. The most responsive health-relevant endpoints were related to xenobiotic metabolism (pregnane X and aryl hydrocarbon receptors), hormone-mediated modes of action (mainly related to the estrogen, glucocorticoid, and antiandrogen activities), reactive modes of action (genotoxicity) and adaptive stress response pathway (oxidative stress response). This study has demonstrated that selected cell-based bioassays are suitable to benchmark water quality and it is recommended to use a purpose-tailored panel of bioassays for routine monitoring.
authors.affiliation: The University of Queensland , National Research Centre for Environmental Toxicology (Entox), 39 Kessels Rd, Brisbane, QLD 4108, Australia.
authors.forename: Beate I
authors.initials: BI
authors.lastname: Escher
authors.forename: Mayumi
authors.initials: M
authors.lastname: Allinson
authors.forename: Rolf
authors.initials: R
authors.lastname: Altenburger
authors.forename: Peter A
authors.initials: PA
authors.lastname: Bain
authors.forename: Patrick
authors.initials: P
authors.lastname: Balaguer
authors.forename: Wibke
authors.initials: W
authors.lastname: Busch
authors.forename: Jordan
authors.initials: J
authors.lastname: Crago
authors.forename: Nancy D
authors.initials: ND
authors.lastname: Denslow
authors.forename: Elke
authors.initials: E
authors.lastname: Dopp
authors.forename: Klara
authors.initials: K
authors.lastname: Hilscherova
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Humpage
authors.forename: Anu
authors.initials: A
authors.lastname: Kumar
authors.forename: Marina
authors.initials: M
authors.lastname: Grimaldi
authors.forename: B Sumith
authors.initials: BS
authors.lastname: Jayasinghe
authors.forename: Barbora
authors.initials: B
authors.lastname: Jarosova
authors.forename: Ai
authors.initials: A
authors.lastname: Jia
authors.forename: Sergei
authors.initials: S
authors.lastname: Makarov
authors.forename: Keith A
authors.initials: KA
authors.lastname: Maruya
authors.forename: Alex
authors.initials: A
authors.lastname: Medvedev
authors.forename: Alvine C
authors.initials: AC
authors.lastname: Mehinto
authors.forename: Jamie E
authors.initials: JE
authors.lastname: Mendez
authors.forename: Anita
authors.initials: A
authors.lastname: Poulsen
authors.forename: Erik
authors.initials: E
authors.lastname: Prochazka
authors.forename: Jessica
authors.initials: J
authors.lastname: Richard
authors.forename: Andrea
authors.initials: A
authors.lastname: Schifferli
authors.forename: Daniel
authors.initials: D
authors.lastname: Schlenk
authors.forename: Stefan
authors.initials: S
authors.lastname: Scholz
authors.forename: Fujio
authors.initials: F
authors.lastname: Shiraishi
authors.forename: Shane
authors.initials: S
authors.lastname: Snyder
authors.forename: Guanyong
authors.initials: G
authors.lastname: Su
authors.forename: Janet Y M
authors.initials: JY
authors.lastname: Tang
authors.forename: Bart
authors.initials: B
authors.lastname: van der Burg
authors.forename: Sander C
authors.initials: SC
authors.lastname: van der Linden
authors.forename: Inge
authors.initials: I
authors.lastname: Werner
authors.forename: Sandy D
authors.initials: SD
authors.lastname: Westerheide
authors.forename: Chris K C
authors.initials: CK
authors.lastname: Wong
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.forename: Bonnie H Y
authors.initials: BH
authors.lastname: Yeung
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhang
authors.forename: Frederic D L
authors.initials: FD
authors.lastname: Leusch
mesh_terms: D000818:Animals; D001315:Australia; D019985:Benchmarking; D001681:Biological Assay; D002606:Charcoal / Q000032:analysis; D060766:Drinking Water / Q000032:analysis* / Q000592:standards; D004967:Estrogens / Q000032:analysis; D005374:Filtration; D066298:In Vitro Techniques; D059027:Recycling; D018675:Toxicity Tests; D062065:Wastewater / Q000032:analysis*; D014867:Water / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis*; D018508:Water Purification; D060753:Water Quality / Q000592:standards*; D015027:Zebrafish
chemical_list: D002606:Charcoal; D060766:Drinking Water; D004967:Estrogens; D062065:Wastewater; D014867:Water; D014874:Water Pollutants, Chemical