title: Chitosan-graphene oxide based aptasensor for the impedimetric detection of lysozyme.
abstract: An impedimetric detection of lysozyme (LYS) was performed for the first time in this study at the surface of chitosan-graphene oxide (CHIT-GO) modified sensor based on the specific interaction process between DNA aptamer and its cognate protein, LYS. The amino linked DNA aptamer (APT) was covalently immobilized without using any chemical agents onto the surface of pencil graphite electrode (PGE). These PGEs are inexpensive and simple to use, and thus, they can be furtherly developed for a single-use application in a portable protein chip device. The electrochemical impedance spectroscopy (EIS) technique was used herein to analyze (i) the surface characterization of unmodified PGE and CHIT-GO modified PGE, and also (ii) the interaction between APT and LYS. The limit of detection (DL) was found as 0.38 μg/mL (equals to 28.53 nM). This impedimetric LYS aptasensor exhibited a higher selectivity toward thrombin and bovine serum albumin, even in the mixture samples.
authors.affiliation: Ege University, Faculty of Pharmacy, Analytical Chemistry Department, Bornova, 35100, Izmir, Turkey. Electronic address: arzum.erdem@ege.edu.tr.
authors.forename: Arzum
authors.initials: A
authors.lastname: Erdem
authors.affiliation: Ege University, Faculty of Pharmacy, Analytical Chemistry Department, Bornova, 35100, Izmir, Turkey.
authors.forename: Ece
authors.initials: E
authors.lastname: Eksin
authors.affiliation: Ege University, Faculty of Pharmacy, Analytical Chemistry Department, Bornova, 35100, Izmir, Turkey; Adnan Menderes University, Faculty of Science, Chemistry Department, 09010, Aydın, Turkey.
authors.forename: Mihrican
authors.initials: M
authors.lastname: Muti
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002417:Cattle; D048271:Chitosan / Q000737:chemistry*; D058266:Dielectric Spectroscopy / Q000379:methods*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009113:Muramidase / Q000032:analysis*; D010087:Oxides / Q000737:chemistry*; D012710:Serum Albumin, Bovine
chemical_list: D052157:Aptamers, Nucleotide; D010087:Oxides; D012710:Serum Albumin, Bovine; D006108:Graphite; D048271:Chitosan; D009113:Muramidase
keywords: Aptamer; Chitosan; Electrochemical impedance spectroscopy; Graphene oxide; Lysozyme