title: A method for high-throughput PCR-based genotyping of larval zebrafish tail biopsies.
abstract: Here we describe a method for high-throughput genotyping of live larval zebrafish as early as 72 h post-fertilization (hpf). Importantly, this technique allows rapid and cost-effective PCR-based genotyping from very small fin biopsies, which regenerate as the embryo develops, thereby allowing researchers to select embryos with desired genotypes to be raised to adulthood.
authors.affiliation: Department of Cardiovascular Science, University of Sheffield, Medical School, Sheffield, United Kingdom.
authors.forename: Robert N
authors.initials: RN
authors.lastname: Wilkinson
authors.affiliation: Department of Biomedical Science/ MRC Centre for Developmental and Biomedical Genetics, University of Sheffield, Sheffield, United Kingdom.
authors.forename: Stone
authors.initials: S
authors.lastname: Elworthy
authors.affiliation: Department of Biomedical Science/ MRC Centre for Developmental and Biomedical Genetics, University of Sheffield, Sheffield, United Kingdom.
authors.forename: Philip W
authors.initials: PW
authors.lastname: Ingham
authors.affiliation: Department of Biomedical Science/ MRC Centre for Developmental and Biomedical Genetics, University of Sheffield, Sheffield, United Kingdom.
authors.forename: Fredericus J M
authors.initials: FJ
authors.lastname: van Eeden
mesh_terms: D000818:Animals; D001706:Biopsy / Q000662:veterinary; D060005:Genotyping Techniques; D059014:High-Throughput Nucleotide Sequencing; D007814:Larva / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D013623:Tail / Q000473:pathology; D015027:Zebrafish / Q000235:genetics* / Q000254:growth & development
keywords: PCR; embryo; fin clip; genotyping; zebrafish