title: Protection from renal fibrosis, putative role of TRIB3 gene silencing.
abstract: BACKGROUND
  Renal fibrosis is thought to be the common pathway in most cases of chronic kidney disease. Recently, TRIB3 was found to play an important role in progression of cardiac fibrosis in an insulin-resistant state. We investigated whether TRIB3 might participate in the pathogenesis of renal fibrosis in insulin-resistant rats.
  
  
  METHODS
  We randomly separated 40 male Sprague-Dawley into 4 groups for treatment (n = 10 each): control and high-fat diet (HFD) with TRIB3 siRNA adenovirus transfection, vehicle transfection or HFD alone. Insulin resistance markers were measured. Renal tissues were stained with hematoxylin and eosin, Masson's trichrome and periodic acid-Schiff.
  
  
  RESULTS
  Rats with HFD showed insulin resistance and TRIB3 overexpression. Upregulated TRIB3 expression could induce renal fibrosis accompanied by increased phosphorylation of extracellular signal-regulated kinase (ERK). Also, TRIB3 siRNA knockdown could ameliorate renal fibrosis, which was accompanied by decreased phosphorylation of ERK.
  
  
  CONCLUSIONS
  TRIB3 gene silencing can attenuate renal fibrosis for beneficial effect on the development of renal fibrosis in chronic kidney disease in rat.
authors.affiliation: Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Department of Cardiology, Qilu Hospital of Shandong University, Ji'nan 250012, PR China; Department of Cardiology, Qilu Hospital of Shandong University, Ji'nan 250012, PR China.
authors.forename: Wen-yuan
authors.initials: WY
authors.lastname: Ding
authors.affiliation: Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Department of Cardiology, Qilu Hospital of Shandong University, Ji'nan 250012, PR China; Department of Cardiology, Qilu Hospital of Shandong University, Ji'nan 250012, PR China.
authors.forename: Wen-bo
authors.initials: WB
authors.lastname: Li
authors.affiliation: Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Department of Cardiology, Qilu Hospital of Shandong University, Ji'nan 250012, PR China; Department of Cardiology, Qilu Hospital of Shandong University, Ji'nan 250012, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ti
authors.affiliation: Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Department of Cardiology, Qilu Hospital of Shandong University, Ji'nan 250012, PR China; Department of Cardiology, Qilu Hospital of Shandong University, Ji'nan 250012, PR China.
authors.forename: Xiu-ping
authors.initials: XP
authors.lastname: Bi
authors.affiliation: Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Department of Cardiology, Qilu Hospital of Shandong University, Ji'nan 250012, PR China; Department of Cardiology, Qilu Hospital of Shandong University, Ji'nan 250012, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.affiliation: Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Department of Cardiology, Qilu Hospital of Shandong University, Ji'nan 250012, PR China; Geriatrics, Qilu Hospital of Shandong University, Ji'nan 250012, PR China.
authors.forename: Zhi-hao
authors.initials: ZH
authors.lastname: Wang
authors.affiliation: Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Department of Cardiology, Qilu Hospital of Shandong University, Ji'nan 250012, PR China; Department of Cardiology, Qilu Hospital of Shandong University, Ji'nan 250012, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Department of Cardiology, Qilu Hospital of Shandong University, Ji'nan 250012, PR China; Department of Cardiology, Qilu Hospital of Shandong University, Ji'nan 250012, PR China. Electronic address: zhangweisdu@gmail.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Department of Cardiology, Qilu Hospital of Shandong University, Ji'nan 250012, PR China; Department of Cardiology, Qilu Hospital of Shandong University, Ji'nan 250012, PR China. Electronic address: zhongmingzm@gmail.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhong
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D059305:Diet, High-Fat / Q000009:adverse effects*; D004195:Disease Models, Animal; D048049:Extracellular Signal-Regulated MAP Kinases; D005355:Fibrosis / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D020868:Gene Silencing; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007333:Insulin Resistance; D007674:Kidney Diseases / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D008297:Male; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors* / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012097:Repressor Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics
chemical_list: D018797:Cell Cycle Proteins; D034741:RNA, Small Interfering; D012097:Repressor Proteins; C474831:TRIB3 protein, human; D017346:Protein Serine-Threonine Kinases; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: MAPK; Renal fibrosis; TRIB3; siRNA