title: Molecular characterization of Chilli leaf curl virus and satellite molecules associated with leaf curl disease of Amaranthus spp.
abstract: Amaranthus, collectively known as amaranth, is an annual or short-lived perennial plant used as leafy vegetables, cereals and for ornamental purposes in many countries including India. During 2011, leaf samples of Amaranthus plants displaying leaf curling, leaf distortion, leaf crinkling and yellow leaf margins were collected from Banswara district, Rajasthan in India. Full-length clones of a monopartite begomovirus, a betasatellite and an alphasatellite were characterized. The complete nucleotide sequence of the isolated begomovirus features as a typical 'Old World' begomovirus with the highest nucleotide per cent identity with Chilli leaf curl virus and hence, considered as an isolate of Chilli leaf curl virus. The complete nucleotide sequences of betasatellite and alphasatellite possess maximum nucleotide identity with Tomato yellow leaf curl Thailand betasatellite and Chilli leaf curl alphasatellite, respectively. This is the first report of the association of chilli-infecting begomovirus and satellite molecules infecting a new host, Amaranthus, causing leaf curl disease.
authors.affiliation: Molecular Virology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110 067, India.
authors.forename: B
authors.initials: B
authors.lastname: George
authors.forename: R Vinoth
authors.initials: RV
authors.lastname: Kumar
authors.forename: S
authors.initials: S
authors.lastname: Chakraborty
mesh_terms: D027721:Amaranthus / Q000821:virology*; D001483:Base Sequence; D053839:Begomovirus / Q000235:genetics* / Q000472:pathogenicity; D017931:DNA Primers; D004276:DNA, Satellite / Q000235:genetics*; D005814:Genes, Viral; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004276:DNA, Satellite