title: Characterization of a novel melt curve by use of the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay: frequencies of this novel (low) melt curve and commonly encountered (intermediate) melt curves.
abstract: We characterize a novel probe binding-site polymorphism detectable solely by melt curve analysis using the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay. The frequencies of this novel (47°C) and previously described intermediate (60 to 62°C) melt curves were 0.016% and 4.9%, respectively.
authors.affiliation: Department of Clinical Pathology, Beaumont Health System, Royal Oak, Michigan, USA.
authors.forename: Bobby L
authors.initials: BL
authors.lastname: Boyanton
authors.forename: Amro
authors.initials: A
authors.lastname: Almradi
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Espy
authors.forename: Anne E
authors.initials: AE
authors.lastname: Prada
authors.forename: John P
authors.initials: JP
authors.lastname: Gibson
authors.forename: Bobbi S
authors.initials: BS
authors.lastname: Pritt
mesh_terms: D005260:Female; D006561:Herpes Simplex / Q000175:diagnosis* / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018258:Herpesvirus 2, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D011110:Polymorphism, Genetic; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D044366:Transition Temperature; D014773:Virology / Q000379:methods*; D055815:Young Adult