title: Biogenic nanopalladium based remediation of chlorinated hydrocarbons in marine environments.
abstract: Biogenic catalysts have been studied over the last 10 years in freshwater and soil environments, but neither their formation nor their application has been explored in marine ecosystems. The objective of this study was to develop a biogenic nanopalladium-based remediation method for reducing chlorinated hydrocarbons from marine environments by employing indigenous marine bacteria. Thirty facultative aerobic marine strains were isolated from two contaminated sites, the Lagoon of Mar Chica, Morocco, and Priolo Gargallo Syracuse, Italy. Eight strains showed concurrent palladium precipitation and biohydrogen production. X-ray diffraction and thin section transmission electron microscopy analysis indicated the presence of metallic Pd nanoparticles of various sizes (5-20 nm) formed either in the cytoplasm, in the periplasmic space, or extracellularly. These biogenic catalysts were used to dechlorinate trichloroethylene in simulated marine environments. Complete dehalogenation of 20 mg L(-1) trichloroethylene was achieved within 1 h using 50 mg L(-1) biogenic nanopalladium. These biogenic nanoparticles are promising developments for future marine bioremediation applications.
authors.affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University , Coupure Links 653, B-9000 Gent, Belgium.
authors.forename: Baharak
authors.initials: B
authors.lastname: Hosseinkhani
authors.forename: Tom
authors.initials: T
authors.lastname: Hennebel
authors.forename: Sam
authors.initials: S
authors.lastname: Van Nevel
authors.forename: Stephanie
authors.initials: S
authors.lastname: Verschuere
authors.forename: Michail M
authors.initials: MM
authors.lastname: Yakimov
authors.forename: Simone
authors.initials: S
authors.lastname: Cappello
authors.forename: Mohamed
authors.initials: M
authors.lastname: Blaghen
authors.forename: Nico
authors.initials: N
authors.lastname: Boon
mesh_terms: D001673:Biodegradation, Environmental; D002384:Catalysis; D045285:Desulfovibrio desulfuricans / Q000378:metabolism*; D054879:Halogenation; D006843:Hydrocarbons, Chlorinated / Q000302:isolation & purification*; D007558:Italy; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D010165:Palladium / Q000737:chemistry* / Q000302:isolation & purification; D010316:Particle Size; D020592:Shewanella / Q000378:metabolism*; D014241:Trichloroethylene / Q000302:isolation & purification; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*; D014961:X-Ray Diffraction
chemical_list: D006843:Hydrocarbons, Chlorinated; D014874:Water Pollutants, Chemical; D014241:Trichloroethylene; D010165:Palladium