title: Effects of high-pressure carbonation on intracellular ATP and NADH levels and DNA damage in Escherichia coli cells.
abstract: In order to understand the microbial inactivation mechanism of high-pressure carbonation (HPC), we examined the changes in the activity of the respiratory chain and DNA damage in Escherichia coli cells. HPC was performed under 1-6 MPa at 30°C for 1 min. The increase in CO2 pressure decreased the number of viable cells of E. coli, intracellular ATP, and intracellular NADH, and increased the number of apurinic/apyrimidinic sites. These results indicate that HPC has a detrimental effect on the functioning of the respiratory chain in E. coli and induces DNA damage, which could result in the death of the bacterial cells.
authors.affiliation: Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University.
authors.forename: Wannaporn
authors.initials: W
authors.lastname: Klangpetch
authors.forename: Seiji
authors.initials: S
authors.lastname: Noma
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Igura
authors.forename: Mitsuya
authors.initials: M
authors.lastname: Shimoda
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D002245:Carbon Dioxide / Q000633:toxicity*; D004249:DNA Damage / Q000187:drug effects*; D004926:Escherichia coli / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology*; D006874:Hydrostatic Pressure; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D050296:Microbial Viability / Q000187:drug effects; D009243:NAD / Q000032:analysis*; D013696:Temperature; D013997:Time Factors
chemical_list: D009243:NAD; D002245:Carbon Dioxide; D000255:Adenosine Triphosphate