title: Prevalence and molecular characterisation of Streptococcus pneumoniae serotype 6C in Queensland, Australia.
abstract: Streptococcus pneumoniae serotype 6C was first identified in 2007, although retrospective studies have since identified serotype 6C among stored isolates dating back to 1962. We investigated the incidence and genetic diversity of serotype 6C strains isolated from Queensland patients between 2001 and 2011. Isolates were identified by Quellung reaction and antimicrobial susceptibility testing was performed. The incidence of serotype 6C among serogroup 6 Queensland invasive pneumococcal disease increased from 6.8% (2001-2004) to 39% (2005-2010) of serogroup 6 isolates (P = 0). Genetic diversity of Queensland 6C isolates was high, with molecular analysis identifying 19 sequence types by multi-locus sequence typing, and 35 types by multi-locus variable-number tandem repeat analysis.
authors.affiliation: Public Health Microbiology, Communicable Disease, Queensland Health Forensic and Scientific Services, Archerfield Queensland 4108, Australia. Electronic address: megan_staples@health.qld.gov.au.
authors.forename: Megan
authors.initials: M
authors.lastname: Staples
authors.affiliation: Public Health Microbiology, Communicable Disease, Queensland Health Forensic and Scientific Services, Archerfield Queensland 4108, Australia.
authors.forename: Amy V
authors.initials: AV
authors.lastname: Jennison
authors.affiliation: Public Health Microbiology, Communicable Disease, Queensland Health Forensic and Scientific Services, Archerfield Queensland 4108, Australia.
authors.forename: Lawrence
authors.initials: L
authors.lastname: Ariotti
authors.affiliation: Public Health Microbiology, Communicable Disease, Queensland Health Forensic and Scientific Services, Archerfield Queensland 4108, Australia.
authors.forename: Vicki
authors.initials: V
authors.lastname: Hicks
authors.affiliation: Public Health Microbiology, Communicable Disease, Queensland Health Forensic and Scientific Services, Archerfield Queensland 4108, Australia.
authors.forename: Rikki M A
authors.initials: RM
authors.lastname: Graham
authors.affiliation: Public Health Microbiology, Communicable Disease, Queensland Health Forensic and Scientific Services, Archerfield Queensland 4108, Australia.
authors.forename: Helen V
authors.initials: HV
authors.lastname: Smith
mesh_terms: D004269:DNA, Bacterial / Q000302:isolation & purification; D024901:Drug Resistance, Multiple, Bacterial; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D011008:Pneumococcal Infections / Q000453:epidemiology*; D015995:Prevalence; D011793:Queensland / Q000453:epidemiology; D017422:Sequence Analysis, DNA; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial
keywords: PCV7; Pneumococcal; Serotype 6C; Streptococcus pneumoniae