title: Septin 7: actin cross-organization is required for axonal association of Schwann cells.
abstract: Myelin sheaths present two distinct domains: compacted myelin spirals and flanking non-compacted cytoplasmic channels, where lipid and protein segregation is established by unknown mechanisms. Septins, a conserved family of membrane and cytoskeletal interacting GTPases, form intracellular diffusion barriers during cell division and neurite extension and are expressed in myelinating cells. Septins, particularly septin 7 (Sept7), the central constituent of septin polymers, are associated with the cytoplasmic channels of myelinating cells. Here we show that Schwann cells deprived of Sept7 fail to wrap around axons from dorsal root ganglion neurons and exhibit disorganization of the actin cytoskeleton. Likewise, Sept7 distribution is dependent on microfilament but not microtubule organization.
authors.affiliation: Laboratorio de Biología Celular de Glias, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago, Chile, alejroth@uchile.cl.
authors.forename: Alejandro D
authors.initials: AD
authors.lastname: Roth
authors.affiliation: Montreal Neurological Institute, McGill University, Montreal, Canada.
authors.forename: Dalinda
authors.initials: D
authors.lastname: Liazoghli
authors.affiliation: Laboratorio de Biología Celular de Glias, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago, Chile, alejroth@uchile.cl.
authors.forename: Felipe
authors.initials: F
authors.lastname: Perez De Arce
authors.affiliation: Montreal Neurological Institute, McGill University, Montreal, Canada.
authors.forename: David R
authors.initials: DR
authors.lastname: Colman
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D001369:Axons / Q000737:chemistry* / Q000502:physiology; D009186:Myelin Sheath / Q000737:chemistry / Q000502:physiology; D009474:Neurons; D011817:Rabbits; D012583:Schwann Cells / Q000737:chemistry* / Q000502:physiology; D058112:Septins / Q000378:metabolism*
chemical_list: D000199:Actins; D058112:Septins