title: Introduction of antioxidant-loaded liposomes into endothelial cell surfaces through DNA hybridization.
abstract: Ischemia-reperfusion damage is a problem in organ transplantation. Reactive oxygen species are produced in cells by blood-mediated reactions at the time of blood reperfusion. In this study, we developed a method to immobilize and internalize antioxidants in endothelial cells, using vitamin E-loaded liposomes. The liposomes loaded with vitamin E and human umbilical vein endothelial cells (HUVECs) were modified with poly(ethylene glycol)-phospholipid conjugates carrying 20-mer of deoxyadenylic acid (oligo(dA)₂₀) and 20-mer of complementary deoxythymidylic acid (oligo(dT)₂₀), respectively. The liposomes were effectively immobilized on HUVECs through DNA hybridization between oligo(dA)₂₀ and oligo(dT)₂₀. The liposomes loaded with vitamin E were gradually internalized into HUVECs. Then, the cells were treated with antimycin A to induce oxidative stress. We found the amount of reactive oxygen species was greatly reduced in HUVECs carrying vitamin E-loaded liposomes.
authors.affiliation: Department of Reparative Materials, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-Cho, Shogoin, Sakyo-Ku, Kyoto 606-8507, Japan.
authors.forename: Sho
authors.initials: S
authors.lastname: Deno
authors.affiliation: Department of Reparative Materials, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-Cho, Shogoin, Sakyo-Ku, Kyoto 606-8507, Japan.
authors.forename: Naohiro
authors.initials: N
authors.lastname: Takemoto
authors.affiliation: Department of Reparative Materials, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-Cho, Shogoin, Sakyo-Ku, Kyoto 606-8507, Japan. Electronic address: iwata@frontier.kyoto-u.ac.jp.
authors.forename: Hiroo
authors.initials: H
authors.lastname: Iwata
mesh_terms: D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D008081:Liposomes / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species; D015427:Reperfusion Injury
chemical_list: D008081:Liposomes; D017382:Reactive Oxygen Species
keywords: DNA hybridization; Endothelial cell; Ischemia; Liposome; Poly(ethylene glycol)–phospholipid; Reperfusion injury; Surface modification; Vitamin E