title: Maternal circulating dendritic cell subtypes at delivery and during the 1-year postpartum period.
abstract: OBJECTIVE
  Dendritic cells (DCs) play an important role in maintaining pregnancy by inducing tolerance toward the fetus. Such an immunologic change in the mother should be restored to normal after delivery, but few studies have reported postpartum maternal immune recovery, in terms of the types circulating DCs.
  
  
  METHODS
  The level of each DC subtype and HLA-DR-positive immunoreactivity of the blood from 29 pregnant women with uncomplicated labor was serially analyzed by flowcytometry at delivery and at 1.5, 6, and 12 months after delivery. DC subtypes were characterized as myeloid, lymphoid, and less differentiated (ldDC). Mean fluorescence intensity (MFI) was evaluated for HLA-DR expression for each DC subtype.
  
  
  RESULTS
  The total number and the percentage of DCs at delivery were lower than those at 12 months postpartum. The ldDC fractions were significantly higher at delivery and at 1.5 months than at 12 months postpartum. The MFI of HLA-DR expression on ldDCs at delivery was lower than that at 12 months postpartum. The myeloid-to-lymphoid DC ratio did not differ over the 1-year postpartum period.
  
  
  CONCLUSIONS
  The maternal alteration in DCs rapidly normalized within 1.5 months, except for the ldDC fraction, which persisted between 1.5 and 6 months after delivery.
authors.affiliation: Department of Laboratory Medicine, Gyeongsang National University Hospital, Jinju, Korea.
authors.forename: Hye Ryun
authors.initials: HR
authors.lastname: Lee
authors.forename: Byoung Jae
authors.initials: BJ
authors.lastname: Kim
authors.forename: Sue
authors.initials: S
authors.lastname: Shin
authors.forename: Hye Won
authors.initials: HW
authors.lastname: Jeon
authors.forename: Eun Youn
authors.initials: EY
authors.lastname: Roh
authors.forename: Jong Hyun
authors.initials: JH
authors.lastname: Yoon
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Song
mesh_terms: D000328:Adult; D001775:Blood Circulation / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D002469:Cell Separation; D036861:Delivery, Obstetric; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D049590:Postpartum Period / Q000276:immunology*; D011247:Pregnancy; D013997:Time Factors; D023001:Transplantation Tolerance; D055815:Young Adult
chemical_list: D006684:HLA-DR Antigens
keywords: Dendritic cells; mean fluorescence intensity; postpartum