title: [In vitro heart models simulating in vivo cardiac ischemia-reperfusion injury].
abstract: The aim of this study was to compare in vivo and several in vitro cardiac ischemia-reperfusion (I-R) myocardial injury models, and choose a superior in vitro cardiac I-R model. Sprague-Dawley (SD) rats were randomly grouped into in vivo, Langendorff, Langendorff + pacing, and working heart groups. Left anterior descending (LAD) coronary artery was ligated for 60 min and then reperfused for 120 min in in vivo and in vitro rat hearts. Cardiac function and myocardial infarct size were measured by using pressure transducer and TTC/Evans blue double staining, respectively. The results showed that heart rate was greater in in vivo model than those in the three in vitro models. Coronary flows were dropped after LAD ligation and could recover at early phase of releasing LAD ligation in I-R models of the isolated working heart, Langendorff and Langendorff with 300 beats/min of electrical stimulation. Left ventricular end-systolic pressure (LVESP) decreased during ischemia, and partially restored during reperfusion in the three in vitro models. Left ventricular end-diastolic pressure (LVEDP) increased during ischemia in the three in vitro models. LVEDP was significantly higher in the isolated working heart than those in Langendorff models during ischemia, whereafter decreased slowly during reperfusion. LVEDP elevated further in the initiation of reperfusion period and then decreased, but did not recover to normal levels during reperfusion in Langendorff and Langendorff + pacing groups. Left ventricular myocardial infarct size was (60.4 ± 5.4)% in in vivo I-R model, which was significantly higher than that in Langendorff model and the isolated working heart. Notably, there was no significant difference in myocardial infarct size between in vivo model and Langendorff model with electrical stimulation. These results suggest that Langendorff I-R model with 300 beats/min of electrical stimulation can simulate the in vivo I-R myocardial injury.
authors.affiliation: Key Laboratory of Aerospace Medicine, Ministry of Education; Department of Aerospace Physiology, Fourth Military Medical University, Xi'an 710032, China. yuzhib@fmmu.edu.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Jiao
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Yu
authors.forename: Yuan-Ming
authors.initials: YM
authors.lastname: Lu
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Yue
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Yu
mesh_terms: D000818:Animals; D006321:Heart / Q000503:physiopathology*; D006339:Heart Rate; D066298:In Vitro Techniques; D009203:Myocardial Infarction / Q000503:physiopathology; D015428:Myocardial Reperfusion Injury; D051381:Rats; D017207:Rats, Sprague-Dawley