title: Preparation and properties of biomimetic porous nanofibrous poly(L-lactide) scaffold with chitosan nanofiber network by a dual thermally induced phase separation technique.
abstract: A biomimetic nanofibrous poly(L-lactide) scaffold decorated by chitosan nanofiber network inside the macropores was fabricated using a dual thermally induced phase separation technique. The first phase separation was used to build a nanofibrous poly(L-lactide) scaffold with interconnected macropores, where chitosan nanofibers about 500nm in diameter were incorporated via the second phase separation. The content of nanofibrous chitosan was determined to be 5.76 in weight percentage by elemental analysis. The composite scaffold showed the highest protein adsorption of 7225±116 μg/cm(3) and the most hydroxyapatite crystal deposition in the mineralization. Compared with non-nanofibrous poly(L-lactide) scaffold, nanofibrous poly(L-lactide) scaffold exhibited a much faster degradation, but it could be restrained by the introduced chitosan nanofibers. The bone mesenchymal stem cell culture results indicated that the cells would rather attach and stretch along the chitosan nanofibers in the composite scaffold that showed the highest viability and the best cytocompatibility may be attributed to the biomimetic nanofibrous network and good cell affinity of chitosan nanofibers.
authors.affiliation: Department of Materials Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou 510632, China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Guangzhou 510632, China.
authors.forename: Jianhao
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Materials Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou 510632, China.
authors.forename: Wanqing
authors.initials: W
authors.lastname: Han
authors.affiliation: Department of Materials Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou 510632, China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Guangzhou 510632, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Tu
authors.affiliation: Department of Orthopaedics, First Hospital Attached to Jinan University, Guangzhou 510632, China.
authors.forename: Songwei
authors.initials: S
authors.lastname: Huan
authors.affiliation: Department of Materials Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou 510632, China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Guangzhou 510632, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zeng
authors.affiliation: Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Guangzhou 510632, China; Department of Orthopaedics, First Hospital Attached to Jinan University, Guangzhou 510632, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Guangzhou 510632, China; Department of Orthopaedics, First Hospital Attached to Jinan University, Guangzhou 510632, China.
authors.forename: Zhengang
authors.initials: Z
authors.lastname: Cha
authors.affiliation: Department of Materials Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou 510632, China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Guangzhou 510632, China.
authors.forename: Changren
authors.initials: C
authors.lastname: Zhou
mesh_terms: D032701:Biomimetics / Q000379:methods; D001842:Bone and Bones / Q000502:physiology; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; C033616:poly(lactide); D048271:Chitosan; D017886:Durapatite