title: An essential role of small ubiquitin-like modifier (SUMO)-specific Protease 2 in myostatin expression and myogenesis.
abstract: Sentrin/small ubiquitin-like modifier (SUMO)-specific protease 2 (SENP2) has broad de-SUMOylation activities in vitro, which is essential for embryonic heart development. Here, we show that myostatin, a key factor in skeletal muscle development, is markedly reduced in Senp2(-/-) mouse embryonic fibroblast cells and embryos. SENP2 regulates the transcription of myostatin mainly through de-SUMOylation of MEF2A. Silencing SENP2 can reduce myostatin expression and, therefore, promote myogenesis of skeletal muscle. These results reveal the important role of SENP2 in the regulation of myostatin expression and myogenesis.
authors.affiliation: From the Department of Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
authors.forename: Yitao
authors.initials: Y
authors.lastname: Qi
authors.forename: Yong
authors.initials: Y
authors.lastname: Zuo
authors.forename: Edward T H
authors.initials: ET
authors.lastname: Yeh
authors.forename: Jinke
authors.initials: J
authors.lastname: Cheng
mesh_terms: D000818:Animals; D002460:Cell Line; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D064326:MEF2 Transcription Factors / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D024510:Muscle Development / Q000502:physiology*; D055435:Myostatin / Q000096:biosynthesis* / Q000235:genetics; D058207:Sumoylation / Q000502:physiology
chemical_list: D064326:MEF2 Transcription Factors; C578036:Mef2a protein, mouse; C525912:Mstn protein, mouse; D055435:Myostatin; D003546:Cysteine Endopeptidases; C439066:Senp2 protein, mouse
keywords: Cell Signaling; Gene Transcription; Myogenesis; SUMOylation; Transforming Growth Factor β (TGFβ)