title: Hexachlorobenzene and pentachlorobenzene accumulation, metabolism and effect on steroid secretion and on CYP11A1 and CYP19 expression in cultured human placental tissue.
abstract: Hexachlorobenzene and pentachlorobenzene accumulation and the effect on CYP1A1, SULT1A, COMT and steroid secretion in term placental tissue were determined. Explants of placental tissue were exposed to between 0.02 and 2 ng/ml HCBz or PeCBz for 6-72 h. Accumulation was measured by capillary gas chromatography and quadrupole mass spectrometry. CYP1A1, SULT1A, COMT activity and progesterone secretion were analysed by EIA. Protein expression was quantified by Western blot; 6% HCBz and 7% PeCBz were detected in the tissue. Fast induction of CYP1A1 activity and protein expression in the presence of HCBz were observed. HCBz increased, while PeCBz decreased COMT protein expression. The stimulatory effect of HCBz, and the inhibitory of PeCBz on progesterone secretion and CYP11A1 protein expression were noted. Later activation of CYP1A1, inhibition of COMT protein expression and progesterone secretion by PeCBz suggest greater exposure to PeCBz and pointing at PeCBz as the main factor responsible for the disruption of placental function.
authors.affiliation: Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University in Krakow, Gronostajowa 9, 30-387 Kraków, Poland. Electronic address: ewa.gregoraszczuk@uj.edu.pl.
authors.forename: E L
authors.initials: EL
authors.lastname: Gregoraszczuk
authors.affiliation: Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University in Krakow, Gronostajowa 9, 30-387 Kraków, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Ptak
authors.affiliation: Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University in Krakow, Gronostajowa 9, 30-387 Kraków, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Karpeta
authors.affiliation: Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University in Krakow, Gronostajowa 9, 30-387 Kraków, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Fiedor
authors.affiliation: Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University in Krakow, Gronostajowa 9, 30-387 Kraków, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Wróbel
authors.affiliation: Department of Gynecological Endocrinology Collegium Medicum, Jagiellonian University in Krakow, Kopernika 23, 31-501 Kraków, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Milewicz
authors.affiliation: Research Group of Environmental Chemistry, Ecotoxicology & Food Toxicology, Institute of Environmental Sciences & Public Health, University of Gdansk, Sobieskiego 18, 80-952 Gdansk, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Falandysz
mesh_terms: D001141:Aromatase / Q000378:metabolism; D015239:Arylsulfotransferase / Q000378:metabolism; D002394:Catechol O-Methyltransferase / Q000378:metabolism; D002722:Chlorobenzenes / Q000494:pharmacology*; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006581:Hexachlorobenzene / Q000494:pharmacology*; D006801:Humans; D010920:Placenta / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism
chemical_list: D002722:Chlorobenzenes; D011374:Progesterone; D004958:Estradiol; D006581:Hexachlorobenzene; C003060:pentachlorobenzene; D001141:Aromatase; C569463:CYP1A1 protein, human; D019363:Cytochrome P-450 CYP1A1; D002786:Cholesterol Side-Chain Cleavage Enzyme; C588010:COMT protein, human; D002394:Catechol O-Methyltransferase; D015239:Arylsulfotransferase; C117544:SULT1A1 protein, human
keywords: Accumulation; Hexachlorobenzene; Metabolism; Pentachlorobenzene; Placenta; Steroidogenesis