title: Hydrophilic interaction ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (HILIC-UPLC-TQ-MS/MS) in multiple-reaction monitoring (MRM) for the determination of nucleobases and nucleosides in ginkgo seeds.
abstract: In this study, a rapid, simple and sensitive analytical method was developed for the quantitative determination of 20 nucleosides and nucleobases in functional foods at trace levels using hydrophilic interaction ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (HILIC-UPLC-TQ-MS/MS) in multiple-reaction monitoring (MRM) mode. Under optimised chromatographic conditions, good separation of 20 target compounds was achieved using a Waters Acquity UPLC BEH Amide column and gradient elution in 11min. The limits of detection (LODs) and quantification (LOQs) were between 0.02-42.54ng/mL and 0.05-98.18ng/mL for the 20 analytes, respectively. This is the first report about simultaneous analysis of nucleosides and nucleobases in functional foods using this method, which afforded good linearity, precision, repeatability and accuracy. The method developed was successfully applied to quantify target compounds in batches of ginkgo seeds. The method potentially could be used to determine polar and trace-level nucleosides and nucleobases in ginkgo seeds.
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and Jiangsu Key Laboratory for High Technology of TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Guisheng
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and Jiangsu Key Laboratory for High Technology of TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Hanqing
authors.initials: H
authors.lastname: Pang
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and Jiangsu Key Laboratory for High Technology of TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address: yupingtang@njutcm.edu.cn.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and Jiangsu Key Laboratory for High Technology of TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yao
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and Jiangsu Key Laboratory for High Technology of TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Yunhe
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and Jiangsu Key Laboratory for High Technology of TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Shaoqing
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and Jiangsu Key Laboratory for High Technology of TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Guo
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and Jiangsu Key Laboratory for High Technology of TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Qian
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and Jiangsu Key Laboratory for High Technology of TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Shen
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and Jiangsu Key Laboratory for High Technology of TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Yefei
authors.initials: Y
authors.lastname: Qian
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and Jiangsu Key Laboratory for High Technology of TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Shulan
authors.initials: S
authors.lastname: Su
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and Jiangsu Key Laboratory for High Technology of TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210023, China; Department of Analysis of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Jiangsu Shenlong Pharmaceutical Co., Ltd., Yancheng 224200, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Jin
authors.affiliation: Jiangsu Shenlong Pharmaceutical Co., Ltd., Yancheng 224200, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and Jiangsu Key Laboratory for High Technology of TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address: dja@njutcm.edu.cn.
authors.forename: Jin-Ao
authors.initials: JA
authors.lastname: Duan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D020441:Ginkgo biloba / Q000737:chemistry*; D009705:Nucleosides / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D011687:Purines / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009705:Nucleosides; D010936:Plant Extracts; D011687:Purines
keywords: Ginkgo biloba; HILIC-UPLC–TQ-MS/MS; Nucleobases; Nucleosides; Seed