title: Development of sandwich ELISA for detection and quantification of invertebrate major allergen tropomyosin by a monoclonal antibody.
abstract: Muscle protein tropomyosins of invertebrates are major allergens responsible for wide spread allergic reactions against invertebrates such as shellfish and insects. In order to develop a sandwich enzyme-linked immunoadsorbent assay (ELISA) for detection and quantification of the invertebrate pan-allergen tropomyosin, a specific monoclonal antibody (MAb), CE7B2, was produced. We have successfully established a sandwich ELISA for measuring invertebrate tropomyosin concentrations in food and food materials. The sandwich ELISA system using the MAb CE7B2 is a useful tool to detect and quantify levels of tropomyosin in food. The method is also helpful to detect mite and cockroach tropomyosins, the important indoor allergens.
authors.affiliation: Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 1138657, Japan.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of College of Food and Technology, Shanghai Ocean University, Shanghai 201306, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 1138657, Japan; Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo 1088477, Japan. Electronic address: aushio@mail.ecc.u-tokyo.ac.jp.
authors.forename: Hideki
authors.initials: H
authors.lastname: Ushio
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo 1088477, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shiomi
mesh_terms: D000485:Allergens / Q000032:analysis*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D005512:Food Hypersensitivity / Q000517:prevention & control; D007448:Invertebrates / Q000737:chemistry* / Q000276:immunology; D014335:Tropomyosin / Q000032:analysis*
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal; D014335:Tropomyosin
keywords: Food allergy; Invertebrate tropomyosin; Monoclonal antibody; Sandwich ELISA