title: [The effect of middle ear effusion on enzymatic digestion of DNA in middle ear effusions of chronic otitis media with effusion].
abstract: OBJECTIVE
  To determine whether or not the bacterial DNA which was detected by PCR comes from viable bacteria.
  
  
  METHODS
  Observing the affection of middle ear effusion (MEE) on DNA viscosity and enzymatic digestion of DNA.
  
  
  RESULTS
  The middle ear effusion and DNA are stable and DNase 1 rapidly digests DNA. The effusion does not seem to degrade DNA. The middle ear effusion signficantly inhibits DNase 1.
  
  
  CONCLUSIONS
  Middle ear effusion provides an inhibition of the enzymatic digestion of purified DNA. Thus any DNA found in effusion by PCR techniques could well be fossilized remains and chronic otitis media with effusion may not be the bacterial infection.
authors.affiliation: Department of Otolaryngology, the First Affiliated Hospital, Nanjing Medical University.
authors.forename: Peizhong
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Otolaryngology, the First Affiliated Hospital, Nanjing Medical University.
authors.forename: Lei
authors.initials: L
authors.lastname: Cheng
mesh_terms: D000293:Adolescent; D012634:Bodily Secretions; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D004269:DNA, Bacterial / Q000737:chemistry*; D003851:Deoxyribonucleases / Q000037:antagonists & inhibitors*; D004432:Ear, Middle / Q000201:enzymology*; D005260:Female; D006801:Humans; D008297:Male; D010034:Otitis Media with Effusion / Q000201:enzymology*; D014783:Viscosity
chemical_list: D004269:DNA, Bacterial; D003851:Deoxyribonucleases