title: Sequence analysis and heterologous expression of the wool cuticle-degrading enzyme encoding genes in Fusarium oxysporum 26-1.
abstract: Two protease-like proteins, KrtA and KrtC, were identified in Fusarium oxysporum 26-1. Genes coding these proteins, krtA and krtC, were isolated and characterized. Recombinant KrtA (rKrtA) and KrtC (rKrtC) were successfully expressed in Aspergillus oryzae and secreted. The combination of rKrtA and rKrtC completely removed the cuticle of wool fibers.
authors.affiliation: Owari Textile Research Center, Aichi Center for Industry and Science Technology, 35 Miyaura, Mabiki, Yamato-cho, Ichinomiya, Aichi 491-0931, Japan.
authors.forename: Etsushi
authors.initials: E
authors.lastname: Chaya
authors.affiliation: The United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Graduate School of Regional Innovation Studies, Mie University, 1577 Kurimamachiya-cho, Tsu, Mie 514-8507, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Karita
authors.affiliation: Food Research Center, Aichi Center for Industry and Science Technology, 2-1-1 Shinpukuji-cho, Nishi-ku, Nagoya, Aichi 451-0083, Japan. Electronic address: akira_hanya@pref.aichi.lg.jp.
authors.forename: Akira
authors.initials: A
authors.lastname: Hanya
authors.affiliation: Food Research Center, Aichi Center for Industry and Science Technology, 2-1-1 Shinpukuji-cho, Nishi-ku, Nagoya, Aichi 451-0083, Japan.
authors.forename: Shoko
authors.initials: S
authors.lastname: Yoshino-Yasuda
authors.affiliation: Food Research Center, Aichi Center for Industry and Science Technology, 2-1-1 Shinpukuji-cho, Nishi-ku, Nagoya, Aichi 451-0083, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Kitamoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001236:Aspergillus oryzae; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D005670:Fusarium / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000737:chemistry / Q000235:genetics*; D020539:Sequence Analysis, Protein; D012756:Sheep; D014935:Wool / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D005656:Fungal Proteins; D010447:Peptide Hydrolases
keywords: Aspergillus oryzae; Fusarium oxysporum; Heterologous expression; Protease; Wool cuticle