title: Induced autologous stem cell transplantation for treatment of rabbit renal interstitial fibrosis.
abstract: BACKGROUND
  Renal interstitial fibrosis (RIF) is a significant cause of end-stage renal failure. The goal of this study was to characterize the distribution of transplanted induced autologous stem cells in a rabbit model of renal interstitial fibrosis and evaluate its therapeutic efficacy for treatment of renal interstitial fibrosis.
  
  
  METHODS
  A rabbit model of renal interstitial fibrosis was established. Autologous fibroblasts were cultured, induced and labeled with green fluorescent protein (GFP). These labeled stem cells were transplanted into the renal artery of model animals at 8 weeks.
  
  
  RESULTS
  Eight weeks following transplantation of induced autologous stem cells, significant reductions (P < 0.05) were observed in serum creatinine (SCr) (14.8 ± 1.9 mmol/L to 10.1 ± 2.1 mmol/L) and blood urea nitrogen (BUN) (119 ± 22 µmol/L to 97 ± 13 µmol/L), indicating improvement in renal function.
  
  
  CONCLUSIONS
  We successfully established a rabbit model of renal interstitial fibrosis and demonstrated that transplantation of induced autologous stem cells can repair kidney damage within 8 weeks. The repair occurred by both inhibition of further development of renal interstitial fibrosis and partial reversal of pre-existing renal interstitial fibrosis. These beneficial effects lead to the development of normal tissue structure and improved renal function.
authors.affiliation: Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital of Chengdu Military Command, Kunming, China.
authors.forename: Guang-Ping
authors.initials: GP
authors.lastname: Ruan
authors.affiliation: Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital of Chengdu Military Command, Kunming, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Xu
authors.affiliation: Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital of Chengdu Military Command, Kunming, China.
authors.forename: Zi-An
authors.initials: ZA
authors.lastname: Li
authors.affiliation: Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital of Chengdu Military Command, Kunming, China.
authors.forename: Guang-Xu
authors.initials: GX
authors.lastname: Zhu
authors.affiliation: Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital of Chengdu Military Command, Kunming, China.
authors.forename: Rong-Qing
authors.initials: RQ
authors.lastname: Pang
authors.affiliation: Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital of Chengdu Military Command, Kunming, China.
authors.forename: Jin-Xiang
authors.initials: JX
authors.lastname: Wang
authors.affiliation: Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital of Chengdu Military Command, Kunming, China.
authors.forename: Xue-Min
authors.initials: XM
authors.lastname: Cai
authors.affiliation: Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital of Chengdu Military Command, Kunming, China.
authors.forename: Jie
authors.initials: J
authors.lastname: He
authors.affiliation: Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital of Chengdu Military Command, Kunming, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Yao
authors.affiliation: Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital of Chengdu Military Command, Kunming, China.
authors.forename: Guang-Hong
authors.initials: GH
authors.lastname: Ruan
authors.affiliation: Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital of Chengdu Military Command, Kunming, China.
authors.forename: Xin-Ming
authors.initials: XM
authors.lastname: Xu
authors.affiliation: Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital of Chengdu Military Command, Kunming, China.
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Pan
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D005347:Fibroblasts / Q000166:cytology; D005355:Fibrosis; D057026:Induced Pluripotent Stem Cells / Q000637:transplantation*; D007668:Kidney / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology; D009395:Nephritis, Interstitial / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D009929:Organ Size; D011817:Rabbits; D033581:Stem Cell Transplantation; D015899:Tomography, Emission-Computed, Single-Photon; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014182:Transplantation, Autologous
chemical_list: D053773:Transforming Growth Factor beta1