title: Effects of reticuloendotheliosis virus infection on cytokine production in SPF chickens.
abstract: Infection with reticuloendotheliosis virus (REV), a gammaretrovirus in the Retroviridae family, can result in immunosuppression and subsequent increased susceptibility to secondary infections. The effects of REV infection on expression of mRNA for cytokine genes in chickens have not been completely elucidated. In this study, using multiplex branched DNA (bDNA) technology, we identified molecular mediators that participated in the regulation of the immune response during REV infection in chickens. Cytokine and chemokine mRNA expression levels were evaluated in the peripheral blood mononuclear cells (PBMCs). Expression levels of interleukin (IL)-4, IL-10, IL-13 and tumor necrosis factor (TNF)-α were significantly up-regulated while interferon (IFN)-α, IFN-β, IFN-γ, IL-1β, IL-2, IL-3, IL-15, IL-17F, IL-18 and colony-stimulating factor (CSF)-1 were markedly decreased in PBMCs at all stages of infection. Compared with controls, REV infected chickens showed greater expression levels of IL-8 in PBMCs 21 and 28 days post infection. In addition, REV regulates host immunity as a suppressor of T cell proliferative responses. The results in this study will help us to understand the host immune response to virus pathogens.
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China ; National Engineering Research Center of Veterinary Biologics, Harbin, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Xue
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China ; National Engineering Research Center of Veterinary Biologics, Harbin, China.
authors.forename: Xingming
authors.initials: X
authors.lastname: Shi
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China ; National Engineering Research Center of Veterinary Biologics, Harbin, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China ; National Engineering Research Center of Veterinary Biologics, Harbin, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Cui
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China ; National Engineering Research Center of Veterinary Biologics, Harbin, China.
authors.forename: Shunlei
authors.initials: S
authors.lastname: Hu
authors.affiliation: Animal Health Laboratory, Department of Primary Industries, Parks, Water and Environment, Tasmania, Australia.
authors.forename: Xianlan
authors.initials: X
authors.lastname: Cui
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China ; National Engineering Research Center of Veterinary Biologics, Harbin, China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D002645:Chickens; D016207:Cytokines / Q000096:biosynthesis*; D011201:Poultry Diseases / Q000378:metabolism*; D001356:Reticuloendotheliosis virus; D012192:Retroviridae Infections / Q000662:veterinary*
chemical_list: D016207:Cytokines