title: Poly(amido-amine)-based hydrogels with tailored mechanical properties and degradation rates for tissue engineering.
abstract: Poly(amido-amine) (PAA) hydrogels containing the 2,2-bisacrylamidoacetic acid-4-amminobutyl guanidine monomeric unit have a known ability to enhance cellular adhesion by interacting with the arginin-glycin-aspartic acid (RGD)-binding αVβ3 integrin, expressed by a wide number of cell types. Scientific interest in this class of materials has traditionally been hampered by their poor mechanical properties and restricted range of degradation rate. Here we present the design of novel biocompatible, RGD-mimic PAA-based hydrogels with wide and tunable degradation rates as well as improved mechanical and biological properties for biomedical applications. This is achieved by radical polymerization of acrylamide-terminated PAA oligomers in both the presence and absence of 2-hydroxyethylmethacrylate. The degradation rate is found to be precisely tunable by adjusting the PAA oligomer molecular weight and acrylic co-monomer concentration in the starting reaction mixture. Cell adhesion and proliferation tests on Madin-Darby canine kidney epithelial cells show that PAA-based hydrogels have the capacity to promote cell adhesion up to 200% compared to the control. Mechanical tests show higher compressive strength of acrylic chain containing hydrogels compared to traditional PAA hydrogels.
authors.affiliation: Fondazione Filarete, Viale Ortles 22/4, 20139 Milano, Italy. Electronic address: federico.martello@fondazionefilarete.com.
authors.forename: Federico
authors.initials: F
authors.lastname: Martello
authors.affiliation: SEMM, European School of Molecular Medicine, Campus IFOM-IEO, Via Adamello 16, 20139 Milano, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Tocchio
authors.affiliation: Fondazione Filarete, Viale Ortles 22/4, 20139 Milano, Italy.
authors.forename: Margherita
authors.initials: M
authors.lastname: Tamplenizza
authors.affiliation: Fondazione Filarete, Viale Ortles 22/4, 20139 Milano, Italy.
authors.forename: Irini
authors.initials: I
authors.lastname: Gerges
authors.affiliation: Fondazione Filarete, Viale Ortles 22/4, 20139 Milano, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Pistis
authors.affiliation: Fondazione Filarete, Viale Ortles 22/4, 20139 Milano, Italy.
authors.forename: Rossella
authors.initials: R
authors.lastname: Recenti
authors.affiliation: Dipartimento di Scienze Biomediche, Chirurgiche ed Odontoiatriche Università degli Studi di Milano, Via R. Galeazzi 4, 20161 Milano, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Bortolin
authors.affiliation: Dipartimento di Scienze Biomediche, Chirurgiche ed Odontoiatriche Università degli Studi di Milano, Via R. Galeazzi 4, 20161 Milano, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Del Fabbro
authors.affiliation: Fondazione Filarete, Viale Ortles 22/4, 20139 Milano, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Argentiere
authors.affiliation: Fondazione Filarete, Viale Ortles 22/4, 20139 Milano, Italy; CIMaINa, Dipartimento di Fisica, Università degli Studi di Milano, Via Celoria 16, 20133 Milano, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Milani
authors.affiliation: Fondazione Filarete, Viale Ortles 22/4, 20139 Milano, Italy; CIMaINa, Dipartimento di Fisica, Università degli Studi di Milano, Via Celoria 16, 20133 Milano, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Lenardi
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004285:Dogs; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D061985:Madin Darby Canine Kidney Cells; D055595:Mechanical Phenomena / Q000187:drug effects*; D011073:Polyamines / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D011102:Polyhydroxyethyl Methacrylate / Q000494:pharmacology; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000199:Actins; D020100:Hydrogels; C531249:Poly(amidoamine); D011073:Polyamines; D011102:Polyhydroxyethyl Methacrylate
keywords: Biomimetics; Hydrogels; Structure–property relationship; Tissue engineering