title: Rapid and direct analysis of statins in human plasma by column-switching liquid chromatography with restricted-access material.
abstract: This study presents the development of a column-switching liquid chromatographic method with direct injection of human plasma for simultaneous determination of four statins (lovastatin, pravastatin, rosuvastatin and simvastatin), the main class of drugs used in the treatment of hyperlipidemia. By using a C18 (30 mm × 4.6 mm, 15 μm) a lab made bovine serum albumin restricted access material (RAM) column was prepared and compared with a commercial alquil-diol silica RAM column (C18, 25 mm × 4.0 mm, 25 μm) in terms of their protein exclusion capacity and micromolecules retention. Foreflush and backflush modes were compared for both RAM columns to the number of theoretical plates, asymmetry, resolution and chromatographic run time. The developed method was validated in the range from 125 to 876 ng mL(-1) for lovastatin, rosuvastatin and simvastatin, and from 500 to 2000 ng mL(-1) for pravastatin, presenting selectivity, precision and accuracy intra and inter-run. Total analysis time (sample preparation and chromatographic separation) was only 16 min when the backflush mode was employed in the column-switching system.
authors.affiliation: Departamento de Produtos Farmacêuticos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: Vinicius Freire
authors.initials: VF
authors.lastname: Fagundes
authors.affiliation: Departamento de Produtos Farmacêuticos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: Camila Prado
authors.initials: CP
authors.lastname: Leite
authors.affiliation: Departamento de Produtos Farmacêuticos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: Gerson Antonio
authors.initials: GA
authors.lastname: Pianetti
authors.affiliation: Departamento de Produtos Farmacêuticos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil. Electronic address: cfernandes@farmacia.ufmg.br.
authors.forename: Christian
authors.initials: C
authors.lastname: Fernandes
mesh_terms: D000596:Amino Acids / Q000097:blood* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D015394:Molecular Structure
chemical_list: D000596:Amino Acids; C013165:statine
keywords: Column-switching; Online sample preparation; Restricted access material; Statins