title: The surface carbohydrates of the Echinococcus granulosus larva interact selectively with the rodent Kupffer cell receptor.
abstract: The larvae of the cestodes belonging to the genus Echinococcus dwell primarily in mammalian liver. They are protected by the laminated layer (LL), an acellular mucin-based structure. The glycans decorating these mucins constitute the overwhelming majority of molecules exposed by these larvae to their hosts. However, their decoding by host innate immunity has not been studied. Out of 36 mammalian innate receptors with carbohydrate-binding domains, expressed as Fc fusions, only the mouse Kupffer cell receptor (KCR; CLEC4F) bound significantly to the Echinococcus granulosus LL mucins. The receptor also bound the Echinococcus multilocularis LL. Out of several synthetic glycans representing Echinococcus LL structures, the KCR bound strongly in particular to those ending in Galα1-4Galβ1-3 or Galα1-4Galβ1-4GlcNAc, both characteristic LL carbohydrate motifs. LL carbohydrates may be optimized to interact with the KCR, expressed only in liver macrophages, cells known to contribute to the tolerogenic antigen presentation that is characteristic of this organ.
authors.affiliation: Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan.
authors.forename: Tsui-Ling
authors.initials: TL
authors.lastname: Hsu
authors.affiliation: Cátedra de Inmunología, Departamento de Biociencias, Facultad de Química, e Instituto de Química Biológica, Facultad de Ciencias, Universidad de la República, Instituto de Higiene, Av. A. Navarro 3051, Montevideo CP 11600, Uruguay.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Lin
authors.affiliation: Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Koizumi
authors.affiliation: University of Würzburg, Institute of Hygiene and Microbiology, Josef-Schneider-Straße 2/E1, 97080 Würzburg, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Brehm
authors.affiliation: Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512, Japan.
authors.forename: Noriyasu
authors.initials: N
authors.lastname: Hada
authors.affiliation: Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan.
authors.forename: Po-Kai
authors.initials: PK
authors.lastname: Chuang
authors.affiliation: Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan.
authors.forename: Chi-Huey
authors.initials: CH
authors.lastname: Wong
authors.affiliation: Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan; Institute of Microbiology & Immunology, Institute of Clinical Medicine & Infection, and Immunity Center, National Yang-Ming University, No. 155, Sec. 2, Linong Street, Taipei 112, Taiwan.
authors.forename: Shie-Liang
authors.initials: SL
authors.lastname: Hsieh
authors.affiliation: Cátedra de Inmunología, Departamento de Biociencias, Facultad de Química, e Instituto de Química Biológica, Facultad de Ciencias, Universidad de la República, Instituto de Higiene, Av. A. Navarro 3051, Montevideo CP 11600, Uruguay. Electronic address: adiaz@fq.edu.uy.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Díaz
mesh_terms: D000818:Animals; D037263:Asialoglycoprotein Receptor / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D048209:Echinococcus granulosus / Q000276:immunology / Q000378:metabolism*; D006790:Host-Parasite Interactions / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate; D007814:Larva; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D009077:Mucins / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D011485:Protein Binding; D011971:Receptors, Immunologic / Q000378:metabolism*
chemical_list: D037263:Asialoglycoprotein Receptor; C072264:Kupffer cell receptor; D009077:Mucins; D011134:Polysaccharides; D011971:Receptors, Immunologic
keywords: Carbohydrate; Echinococcus; Kupffer cell receptor; Kupffer cells; Laminated layer; Liver