title: Production of Sporotrichum thermophile xylanase by solid state fermentation utilizing deoiled Jatropha curcas seed cake and its application in xylooligosachharide synthesis.
abstract: De-oiled Jatropha curcas seed cake, a plentiful by-product of biodiesel industry was used as substrate for the production of a useful xylanase from Sporotrichum thermophile in solid state fermentation. Under the optimized conditions, 1025U xylanase/g (deoiled seed cake) was produced. The xylanase exhibited half life of 4h at 45°C and 71.44min at 50°C respectively. It was stable in a broad pH range of 7.0-11.0. Km and Vmax were 12.54mg/ml and 454.5U/ml/min respectively. S. thermophile xylanase is an endoxylanase free of exoxylanase activity, hence advantageous for xylan hydrolysis to produce xylooligosachharides. Hydrolysis of oat spelt xylan by S. thermophile xylanase yielded 73% xylotetraose, 15.4% xylotriose and 10% xylobiose. The S. thermophile endoxylanase thus seem potentially useful in the food industries.
authors.affiliation: Department of Chemistry, Indian Institute of Technology, New Delhi 110016, India.
authors.forename: Ayesha
authors.initials: A
authors.lastname: Sadaf
authors.affiliation: Department of Chemistry, Indian Institute of Technology, New Delhi 110016, India. Electronic address: skhare@rocketmail.com.
authors.forename: S K
authors.initials: SK
authors.lastname: Khare
mesh_terms: D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002244:Carbon / Q000494:pharmacology; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis*; D005285:Fermentation / Q000187:drug effects; D005965:Glucuronates / Q000096:biosynthesis*; D006813:Humidity; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D031286:Jatropha / Q000737:chemistry*; D007700:Kinetics; D009844:Oligosaccharides / Q000096:biosynthesis*; D010938:Plant Oils / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry*; D013175:Sporothrix / Q000187:drug effects / Q000201:enzymology*; D013379:Substrate Specificity / Q000187:drug effects; D014866:Waste Products; D014990:Xylans / Q000378:metabolism
chemical_list: D005965:Glucuronates; D009844:Oligosaccharides; D010938:Plant Oils; D014866:Waste Products; D014990:Xylans; C570991:xylooligosaccharide; D002244:Carbon; D043364:Endo-1,4-beta Xylanases
keywords: Jatropha curcas; Sporotrichum thermophile; Xylanase; Xylooligosachharides