title: Optimisation of ultrasound-assisted enzymatic extraction of arabinoxylan from wheat bran.
abstract: Arabinoxylan, the major dietary fibre component of wheat bran, is important from both technological and nutritional points of view. In this study, ultrasound-assisted enzymatic extraction technology was first employed to extract arabinoxylan from wheat bran. The process for extraction of arabinoxylan was optimised using response surface methodology, employing a five-level, five-variable central composite rotatable design. The optimum extraction conditions were as follows: raw material concentration, 50g/l, enzyme dose, 4.5g/l, extraction temperature, 50°C; extraction time, 70min; and ultrasonic power, 180W. Under the above mentioned conditions, the experimental yield was 142.6±0.17mg/g of wheat bran, which is well matched with the predictive yield. Ultrasound increased the efficiency of enzymatic treatment with higher extraction yield.
authors.affiliation: Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, 11 Fucheng Road, Beijing 100048, PR China. Electronic address: jingw810@yahoo.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, 11 Fucheng Road, Beijing 100048, PR China.
authors.forename: Baoguo
authors.initials: B
authors.lastname: Sun
authors.affiliation: Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, 11 Fucheng Road, Beijing 100048, PR China.
authors.forename: Yingli
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, 11 Fucheng Road, Beijing 100048, PR China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Zhang
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000295:instrumentation / Q000379:methods*; D001412:Bacillus subtilis / Q000201:enzymology; D001426:Bacterial Proteins / Q000737:chemistry*; D004043:Dietary Fiber / Q000032:analysis*; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry*; D006868:Hydrolysis; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D014908:Triticum / Q000737:chemistry*; D014465:Ultrasonics; D014990:Xylans / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D004043:Dietary Fiber; D010936:Plant Extracts; D014990:Xylans; C085118:arabinoxylan; D043364:Endo-1,4-beta Xylanases
keywords: Arabinoxylan; Central composite rotatable design; Enzymatic extraction; Ultrasound; Wheat bran