title: Oxidative stress response of Blakeslea trispora induced by H₂O₂ during β-carotene biosynthesis.
abstract: The cellular response of Blakeslea trispora to oxidative stress induced by H₂O₂ in shake flask culture was investigated in this study. A mild oxidative stress was created by adding 40 μm of H₂O₂ into the medium after 3 days of the fermentation. The production of β-carotene increased nearly 38 % after a 6-day culture. Under the oxidative stress induced by H₂O₂, the expressions of hmgr, ipi, carG, carRA, and carB involving the β-carotene biosynthetic pathway all increased in 3 h. The aerobic metabolism of glucose remarkably accelerated within 24 h. In addition, the specific activities of superoxide dismutase and catalase were significantly increased. These changes of B. trispora were responses for reducing cell injury, and the reasons for increasing β-carotene production caused by H₂O₂.
authors.affiliation: Department of Biotechnology, College of Life Science and Technology, Institute of Resource Biology and Biotechnology, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Luo
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Huang
authors.forename: Ming-Bo
authors.initials: MB
authors.lastname: Lu
authors.forename: Long-Jiang
authors.initials: LJ
authors.lastname: Yu
mesh_terms: D053898:Biosynthetic Pathways; D002374:Catalase / Q000378:metabolism; D005285:Fermentation; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007218:Industrial Microbiology; D009090:Mucorales / Q000378:metabolism*; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism; D019207:beta Carotene / Q000096:biosynthesis*
chemical_list: D019207:beta Carotene; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase