title: [Determination of 2, 4-toluenediamine in urine by gas chromatography].
abstract: OBJECTIVE
  To establish a method for determining the content of 2,4-toluenediamine, a urinary metabolite of toluene diisocyanate, by gas chromatography.
  
  
  METHODS
  Urine samples were collected, and acidification, extraction, derivatization, separation with a capillary column, and detection with an electron capture detector were performed. The target compound was qualified by retention time and quantified by peak area.
  
  
  RESULTS
  The concentration of 2, 4-toluenediamine showed a linear relationship with peak area within 0.0∼40 ng/ml, with a correlation coefficient 0.9995; the limit of detection was 0.44 ng/ml, the lower limit of quantification was 1.47 ng/ml, the relative standard deviation was 1.85%∼4.05%; the recovery rate was 97.98%∼99.28%.
  
  
  CONCLUSIONS
  The method has the advantages of high sensitivity and high accuracy and can be used for determination of 2, 4-toluenediamine in urine.
authors.affiliation: Tianjin Centers for Disease Control and Prevention, 6th Huayue Street, Tianjin 300011, China.
authors.forename: Wan-chao
authors.initials: WC
authors.lastname: Zhang
authors.forename: Bao-feng
authors.initials: BF
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: E-mail: jstzmrm@163.com.
authors.forename: Rong-ming
authors.initials: RM
authors.lastname: Miao
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D016273:Occupational Exposure / Q000032:analysis*; D010655:Phenylenediamines / Q000652:urine*
chemical_list: D010655:Phenylenediamines; C010914:2,4-diaminotoluene