title: Der p2 recombinant bacille Calmette-Guerin priming of bone marrow-derived dendritic cells suppresses Der p2-induced T helper17 function in a mouse model of asthma.
abstract: OBJECTIVE
  Previous studies have demonstrated that our recombinant bacille Calmette-Guerin (rBCG), which expresses Der p2 in house dust mite (Der p2 rBCG) suppresses asthmatic airway inflammation by regulating the phenotype and function of dendritic cells (DC) and reprogramming T helper (Th) 0 cell differentiation into different T cell (Th1/Th2/Treg) subtypes. However, the exact role of Der p2 rBCG in reprogramming Th17 differentiation and the relevant mechanisms are not known. The aim of this study was to examine whether Der p2 rBCG-mediated inhibition of allergic airway inflammation is mediated by regulating Th17 differentiation in a murine asthma model.
  
  
  METHODS
  Primary mouse bone marrow-derived dendritic cells (BMDC) were infected with Der p2 rBCG and adoptively transferred to Der p2-intranasally sensitized mice. The role of Der p2 rBCG-BMDC on the regulation of airway inflammation and Th17 cell differentiation was assessed.
  
  
  RESULTS
  Adoptive transfer of Der p2 rBCG-BMDC suppressed airway inflammation and mucin secretion. Der p2 rBCG-BMDC inhibited excessive Th17 immune responses but not BCG-BMDC. Furthermore, Der p2 rBCG decreased jagged-2 and increased delta-like-4 expressions on BMDC to a greater extent than BCG.
  
  
  CONCLUSIONS
  These findings suggest that DC plays a key role in Der p2 rBCG-induced immunoregulation. Der p2 rBCG also displayed a potent inhibitory effect on Th17 differentiation, and these findings increase our understanding of the cellular basis of Der p2 BCG-mediated inhibition of asthma.
authors.affiliation: Department of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Shuo-Yao
authors.initials: SY
authors.lastname: Qu
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Ou-Yang
authors.forename: Ya-Long
authors.initials: YL
authors.lastname: He
authors.forename: Zhi-Kui
authors.initials: ZK
authors.lastname: Li
authors.forename: Jie-Ran
authors.initials: JR
authors.lastname: Shi
authors.forename: Li-Qiang
authors.initials: LQ
authors.lastname: Song
authors.forename: Chang-Gui
authors.initials: CG
authors.lastname: Wu
mesh_terms: D000818:Animals; D039741:Antigens, Dermatophagoides / Q000096:biosynthesis / Q000235:genetics*; D060829:Arthropod Proteins / Q000096:biosynthesis / Q000235:genetics*; D001249:Asthma / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D051379:Mice; D008810:Mice, Inbred C57BL; D009163:Mycobacterium bovis / Q000276:immunology / Q000378:metabolism*; D012313:RNA / Q000235:genetics; D058504:Th17 Cells / Q000276:immunology* / Q000378:metabolism
chemical_list: D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; C451163:Dermatophagoides pteronyssinus antigen p 2; D012313:RNA
keywords: Notch signalling; T helper 17; airway inflammation; dendritic cell; recombinant bacille Calmette-Guerin