title: Identification of Arcanobacterium pluranimalium by matrix-assisted laser desorption ionization-time of flight mass spectrometry and, as novel target, by sequencing pluranimaliumlysin encoding gene pla.
abstract: In the present study 13 Arcanobacterium pluranimalium strains isolated from various animal origin could successfully be identified phenotypically by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and genotypically by sequencing 16S rDNA and the pluranimaliumlysin encoding gene pla. The detection of mass spectra by MALDI-TOF MS and the novel genotypic approach using gene pla might help to identify A. pluranimalium in future and might elucidate the role this species plays in infections of animals.
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstr 81, 35392 Gießen, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Balbutskaya
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstr 81, 35392 Gießen, Germany.
authors.forename: O
authors.initials: O
authors.lastname: Sammra
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstr 81, 35392 Gießen, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Nagib
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstr 81, 35392 Gießen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Hijazin
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstr 81, 35392 Gießen, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Alber
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstr 81, 35392 Gießen, Germany. Electronic address: christoph.laemmler@vetmed.uni-giessen.de.
authors.forename: C
authors.initials: C
authors.lastname: Lämmler
authors.affiliation: SAC Consulting Veterinary Services, Drummondhill, Stratherrick Road, Inverness IV2 4JZ, UK.
authors.forename: G
authors.initials: G
authors.lastname: Foster
authors.affiliation: RIPAC-LABOR GmbH, Am Mühlenberg 11, 14476 Potsdam-Golm, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Erhard
authors.affiliation: Animal Health and Veterinary Laboratories Agency, Penrith Regional Laboratory, Merrythought, Calthwaite, Penrith CA11 9RR, UK.
authors.forename: P N
authors.initials: PN
authors.lastname: Wragg
authors.affiliation: Institut für Lebensmittelqualität und-sicherheit, Stiftung Tierärztliche Hochschule Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Abdulmawjood
authors.affiliation: Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität, Frankfurterstr. 85-91, 35392 Gießen, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Prenger-Berninghoff
mesh_terms: D000193:Actinomycetales Infections / Q000382:microbiology* / Q000662:veterinary*; D000818:Animals; D055667:Arcanobacterium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics*; D002417:Cattle; D004285:Dogs; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012756:Sheep; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S
keywords: 16S rDNA; Arcanobacterium pluranimalium; MALDI-TOF MS; Pluranimaliumlysin; pla