title: [Inhibitory effect of PD98059 on MAPK signaling pathway in acute lymphocytic leukemia cells].
abstract: This study was purposed to investigate the effect of blocking Ras/Erk signaling pathway on expression of important transcription factor c-fos, c-jun and TAK1 gene in primary acute lymphocytic leukemia (ALL) cells. The best effective concentration and effect time of PD98059 were screened; the expression levels of c-fos, c-jun and TAK1 in primary cultured cells of normal persons, primary cultured ALL cells and primary cultured ALL cells treated by PD98059 were detected by SYBR GreenI real-time quantitative-PCR. The results showed that before treatment by PD98059 the expression levels of c-fos and TAK1 mRNA were significantly up-regulated in primary cultured ALL cells as compared with primary cultured cells of normal persons (P = 0.014 and P = 0.017 respectively). After treatment by PD98059, the expression levels of c-fos, c-jun mRNA decreased in all 7 serum samples, while expression of TAK1 was down-regulated in 5 samples, and up-regulated in 2 samples. After treatment with PD98059, there was no statistical difference of c-fos, c-jun and TAK1 expression levels in primary cultured ALL cells and primary cultured normal cells. It is concluded that the c-fos and TAK1 activity of primary cultured ALL cells increases, and blocking the Ras/Erk signaling pathway of ALL cells can lead to obvious decrease of important transcription factors c-fos, c-jun, TAK1 genes expression.
authors.affiliation: Department of Hematopathy, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, Henan Province, China.
authors.forename: Qian-Yu
authors.initials: QY
authors.lastname: Li
authors.affiliation: Department of Hematopathy, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, Henan Province, China.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Wei
authors.affiliation: Department of Hematopathy, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, Henan Province, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Hematopathy, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, Henan Province, China. E-mail:jnyinqingsong@163.com.
authors.forename: Qing-Song
authors.initials: QS
authors.lastname: Yin
mesh_terms: D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D014407:Tumor Cells, Cultured; D000099151:MAP Kinase Kinase Kinase 7
chemical_list: D005419:Flavonoids; D020930:MAP Kinase Kinase Kinases; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D000099151:MAP Kinase Kinase Kinase 7; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one