title: Nonnegative mixed-norm convex optimization for mitotic cell detection in phase contrast microscopy.
abstract: This paper proposes a nonnegative mix-norm convex optimization method for mitotic cell detection. First, we apply an imaging model-based microscopy image segmentation method that exploits phase contrast optics to extract mitotic candidates in the input images. Then, a convex objective function regularized by mix-norm with nonnegative constraint is proposed to induce sparsity and consistence for discriminative representation of deformable objects in a sparse representation scheme. At last, a Support Vector Machine classifier is utilized for mitotic cell modeling and detection. This method can overcome the difficulty in feature formulation for deformable objects and is independent of tracking or temporal inference model. The comparison experiments demonstrate that the proposed method can produce competing results with the state-of-the-art methods.
authors.affiliation: School of Electronic Information Engineering, Tianjin University, Tianjin 300072, China.
authors.forename: Anan
authors.initials: A
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Tianjin Normal University, Tianjin 300387, China ; Key Laboratory of Systems Bioengineering, Ministry of Education, Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Hao
authors.affiliation: Key Laboratory of Computer Vision and System, Tianjin University of Technology, Tianjin 300384, China.
authors.forename: Zan
authors.initials: Z
authors.lastname: Gao
authors.affiliation: School of Electronic Information Engineering, Tianjin University, Tianjin 300072, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Su
authors.affiliation: School of Electronic Information Engineering, Tianjin University, Tianjin 300072, China.
authors.forename: Zhaoxuan
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000465:Algorithms; D000818:Animals; D049109:Cell Proliferation; D005188:False Negative Reactions; D005189:False Positive Reactions; D007090:Image Interpretation, Computer-Assisted; D007091:Image Processing, Computer-Assisted; D051379:Mice; D008809:Mice, Inbred C3H; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D008938:Mitosis; D010363:Pattern Recognition, Automated; D012984:Software; D060388:Support Vector Machine