title: Effect of white tea (Camellia sinensis (L.)) extract in the glycolytic profile of Sertoli cell.
abstract: OBJECTIVE
  Many health benefits have been attributed to tea (Camellia sinensis (L.)), and tea infusions are used as dietary agent and included in food supplements. Herein, we report the effect of a white tea (WTEA) extract in Sertoli cell (SC) metabolism. The SC is responsible for the nutritional support of the developing germ cells.
  
  
  METHODS
  An aqueous WTEA extract was prepared and analyzed by (1)H-NMR. Rat SCs were cultured with or without the WTEA extract. mRNA and protein levels of glucose transporters (GLUT1 and GLUT3), phosphofructokinase, lactate dehydrogenase (LDH) and monocarboxylate transporter 4 were determined by qPCR and western blot. LDH activity was assessed and metabolite production/consumption determined by (1)H-NMR.
  
  
  RESULTS
  WTEA-exposed SCs presented decreased protein and mRNA levels of GLUT1 and decreased glucose uptake. However, intracellular LDH activity was increased and SC lactate production was stimulated by the presence of the WTEA extract. Interestingly, alanine production was also found to be stimulated in WTEA extract-exposed SCs.
  
  
  CONCLUSIONS
  WTEA extract altered the glycolytic profile of cultured SCs, stimulating lactate production. Since lactate is used as metabolic substrate and has an anti-apoptotic effect in the developing germ cells, the supplementation with WTEA extract may be advantageous to improve male reproductive health.
authors.affiliation: CICS-UBI, Health Sciences Research Centre, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6200-506, Covilhã, Portugal.
authors.forename: A D
authors.initials: AD
authors.lastname: Martins
authors.forename: M G
authors.initials: MG
authors.lastname: Alves
authors.forename: R L
authors.initials: RL
authors.lastname: Bernardino
authors.forename: T R
authors.initials: TR
authors.lastname: Dias
authors.forename: B M
authors.initials: BM
authors.lastname: Silva
authors.forename: P F
authors.initials: PF
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001786:Blood Glucose / Q000378:metabolism; D002110:Caffeine / Q000032:analysis / Q000494:pharmacology; D002392:Catechin / Q000032:analysis / Q000494:pharmacology; D002478:Cells, Cultured; D051272:Glucose Transporter Type 1 / Q000235:genetics / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects*; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D027501:Monocarboxylic Acid Transporters / Q000235:genetics / Q000378:metabolism; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D010732:Phosphofructokinase-1 / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012098:Reproduction / Q000187:drug effects; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism; D013662:Tea / Q000737:chemistry*
chemical_list: D001786:Blood Glucose; D051272:Glucose Transporter Type 1; D027501:Monocarboxylic Acid Transporters; D009124:Muscle Proteins; D010936:Plant Extracts; D012333:RNA, Messenger; C516342:Slc16a3 protein, rat; C495679:Slc2a1 protein, rat; D013662:Tea; D019344:Lactic Acid; D002110:Caffeine; D002392:Catechin; D007770:L-Lactate Dehydrogenase; D010732:Phosphofructokinase-1