title: A novel model for orthotopic liver transplantation in rats using hepatic rearterialization and biliary extradrainage system.
abstract: BACKGROUND
  Although the rat orthotopic liver transplantation (OLT) model has existed for many years, only a few models can be applied for dynamic bile collection. The aim of this study was to introduce a dependent rat OLT model with hepatic rearterialization and an expediently dynamic bile collection system.
  
  
  METHODS
  Forty-five male Sprague-Dawley rats were divided into the following three groups (n = 15 each): group A, OLT without hepatic rearterialization; group B, OLT with hepatic rearterialization; group C, OLT with hepatic rearterialization and a biliary extradrainage system. In groups B and C, a modified sleeve anastomosis between the donor common hepatic artery and the recipient proper hepatic artery was performed to restore the hepatic artery blood flow. In group C, after hepatic rearterialization, biliary extradrainage and jejunum stoma were performed to reestablish the bile flow, and a waistcoat-like external fixator was introduced to protect this system.
  
  
  RESULTS
  The surgical success rates in groups A, B, and C were 100% (15/15), 93% (14/15), and 93% (14/15), respectively. In groups B and C, the hepatic artery patency rates were 93% and 86% on postoperative day 3 and postoperative day 21, respectively. Also, the liver function and bile duct integrity were preserved better than that in group A. In group C, the biliary extradrainage system was well preserved and bile collection was easily performed.
  
  
  CONCLUSIONS
  The rat OLT model with hepatic rearterialization and a convenient biliary extradrainage system was satisfactory in maintaining the survival rate, hepatic artery patency rate, and recovery of graft function, so it can be applied in various studies after transplantation.
authors.affiliation: Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Jilin
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Shuyun
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Peihao
authors.initials: P
authors.lastname: Wen
authors.affiliation: Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Junwei
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Chongzhi
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Pathology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Huamei
authors.initials: H
authors.lastname: Tang
authors.affiliation: Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China. Electronic address: pengzhihaipzhh@hotmail.com.
authors.forename: Zhihai
authors.initials: Z
authors.lastname: Peng
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism; D017734:Bile Ducts, Extrahepatic / Q000378:metabolism / Q000601:surgery; D001662:Biliary Tract Surgical Procedures / Q000379:methods*; D006085:Graft Survival; D006499:Hepatic Artery / Q000601:surgery*; D008099:Liver / Q000378:metabolism / Q000601:surgery; D008102:Liver Circulation; D016031:Liver Transplantation / Q000379:methods*; D008297:Male; D023421:Models, Animal; D011183:Postoperative Complications / Q000517:prevention & control; D051381:Rats; D017207:Rats, Sprague-Dawley; D014654:Vascular Patency; D014656:Vascular Surgical Procedures / Q000379:methods*
keywords: Biliary extradrainage; Hepatic rearterialization; Liver transplantation; Rat