title: Ambroxol inhibits neutrophil respiratory burst activated by alpha chain integrin adhesion.
abstract: The purpose of the present study was to investigate the possible anti-oxidant effect(s) of Ambroxol on neutrophils activated by ligand-binding of the drug with membrane-associated adhesion integrin CD11a and to estimate dose-response changes in oxygen free radical production. The amount of free radical production by anti-CD11a- and anti-CD4-coated neutrophils stimulated with N-formyl-methionyl-leucyl-phenylalanine (FMLP) and challenged with increasing concentration of Ambroxol, was evaluated within a time frame of 90 minutes. A significant dose-dependent effect response of Ambroxol on O2&#8254; production by cells coated with anti-CD11a antibody was observed. This preliminary study opens a new perspective on the therapeutic role of Ambroxol as an antioxidant drug and for its potential use in controlling oxidative stress, particularly in leukocyte-dependent inflammation.
authors.affiliation: Paediatric Section, Department of Life and Reproduction Sciences, University of Verona, Verona, Italy.
authors.forename: D G
authors.initials: DG
authors.lastname: Peroni
authors.affiliation: Paediatric Section, Department of Life and Reproduction Sciences, University of Verona, Verona, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Moser
authors.affiliation: Paediatric Section, Department of Life and Reproduction Sciences, University of Verona, Verona, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Gallo
authors.affiliation: Paediatric Section, Department of Life and Reproduction Sciences, University of Verona, Verona, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Pigozzi
authors.affiliation: Paediatric Section, Department of Life and Reproduction Sciences, University of Verona, Verona, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Tenero
authors.affiliation: Paediatric Section, Department of Life and Reproduction Sciences, University of Verona, Verona, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Zanoni
authors.affiliation: Paediatric Section, Department of Life and Reproduction Sciences, University of Verona, Verona, Italy.
authors.forename: A L
authors.initials: AL
authors.lastname: Boner
authors.affiliation: Paediatric Section, Department of Life and Reproduction Sciences, University of Verona, Verona, Italy.
authors.forename: G L
authors.initials: GL
authors.lastname: Piacentini
mesh_terms: D000551:Ambroxol / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D040881:CD11a Antigen / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D040881:CD11a Antigen; D000551:Ambroxol; D002118:Calcium