title: Acid lipase from Candida viswanathii: production, biochemical properties, and potential application.
abstract: Influences of environmental variables and emulsifiers on lipase production of a Candida viswanathii strain were investigated. The highest lipase activity (101.1 U) was observed at 210 rpm, pH 6.0, and 27.5°C. Other fermentation parameters analyzed showed considerable rates of biomass yield (Y L/S = 1.381 g/g), lipase yield (Y L/S = 6.892 U/g), and biomass productivity (P X = 0.282 g/h). Addition of soybean lecithin increased lipase production in 1.45-fold, presenting lipase yield (Y L/S ) of 10.061 U/g. Crude lipase presented optimal activity at acid pH of 3.5, suggesting a new lipolytic enzyme for this genus and yeast in general. In addition, crude lipase presented high stability in acid conditions and temperature between 40 and 45°C, after 24 h of incubation in these temperatures. Lipase remained active in the presence of organic solvents maintaining above 80% activity in DMSO, methanol, acetonitrile, ethanol, acetone, 1-propanol, isopropanol, and 2-propanol. Effectiveness for the hydrolysis of a wide range of natural triglycerides suggests that this new acid lipase has high potential application in the oleochemical and food industries for hydrolysis and/or modification of triacylglycerols to improve the nutritional properties.
authors.affiliation: Environmental Studies Center, Universidade Estadual Paulista, CEA/UNESP, Avenida 24-A, 1515 Bela Vista, 13506-900 Rio Claro, SP, Brazil.
authors.forename: Alex Fernando
authors.initials: AF
authors.lastname: de Almeida
authors.affiliation: Environmental Studies Center, Universidade Estadual Paulista, CEA/UNESP, Avenida 24-A, 1515 Bela Vista, 13506-900 Rio Claro, SP, Brazil.
authors.forename: Sâmia Maria
authors.initials: SM
authors.lastname: Tauk-Tornisielo
authors.affiliation: Biochemistry and Microbiology Department, Bioscience Institute, Universidade Estadual Paulista, IB/UNESP, Avenida 24-A, 1515 Bela Vista, 13506-900 Rio Claro, SP, Brazil.
authors.forename: Eleonora Cano
authors.initials: EC
authors.lastname: Carmona
mesh_terms: D018533:Biomass; D002175:Candida / Q000201:enzymology* / Q000378:metabolism*; D005285:Fermentation / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D054709:Lecithins / Q000378:metabolism; D008049:Lipase / Q000096:biosynthesis* / Q000378:metabolism*; D013696:Temperature; D014280:Triglycerides / Q000378:metabolism
chemical_list: D054709:Lecithins; D014280:Triglycerides; D008049:Lipase