title: Postprandial coagulation activation in overweight individuals after weight loss: acute and long-term effects of a high-monounsaturated fat diet and a low-fat diet.
abstract: Diet is important in the prevention of cardiovascular disease, and it has been suggested that a high-MUFA diet is more cardioprotective than a low-fat diet. We hypothesised that the postprandial thrombotic risk profile is improved most favourably by a high-MUFA diet compared with a low-fat diet. This was tested in a parallel intervention trial on overweight individuals (aged 28.4 (SD 4.7) years) randomly assigned to a MUFA-diet (35-45% of energy as fat; >20% as MUFA, n = 21) or a low-fat (LF) diet (20-30% of energy as fat, n = 22) for 6 months after a weight loss of ~10%. All foods were provided free of charge from a purpose-built supermarket. Meal tests designed after the same principles were performed before and after the dietary intervention, and blood samples were collected at 8.00 h (fasting), 12.00 h, and 18.00 h and analysed for factor VII coagulant activity (FVII:C), activated FVII, fibrinogen, prothrombin fragment 1 + 2 (F1 + 2), D-dimer, plasminogen activator inhibitor (PAI:Ag), and thrombin activatable fibrinolysis inhibitor. There were significant postprandial increases in F1 + 2 and D-dimer before and after dietary intervention, with significantly lower values after 6 months. No significant differences were observed between the postprandial changes induced by the two diets. The postprandial decrease in FVII:C and PAI:Ag did not differ before and after intervention, irrespective of the diets. Our findings suggest postprandial coagulation activation in overweight subjects with more pronounced acute than long-term effects. We observed similar effects of the MUFA diet and the LF diet on the postprandial prothrombotic risk profile.
authors.affiliation: Unit for Thrombosis Research, Institute of Public Health, the University of Southern Denmark and Dept. of Clinical Biochemistry, Hospital of South West Denmark, Esbjerg, Denmark. Electronic address: ebladbjerg@health.sdu.dk.
authors.forename: Else-Marie
authors.initials: EM
authors.lastname: Bladbjerg
authors.affiliation: Department of Human Nutrition, Centre for Advanced Food Studies, Faculty of Life Sciences, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Larsen
authors.affiliation: Department of Human Nutrition, Centre for Advanced Food Studies, Faculty of Life Sciences, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Anette
authors.initials: A
authors.lastname: Due
authors.affiliation: Unit for Thrombosis Research, Institute of Public Health, the University of Southern Denmark and Dept. of Clinical Biochemistry, Hospital of South West Denmark, Esbjerg, Denmark.
authors.forename: Jørgen
authors.initials: J
authors.lastname: Jespersen
authors.affiliation: Department of Clinical Biochemistry, Copenhagen University Hospital, Gentofte, Denmark.
authors.forename: Steen
authors.initials: S
authors.lastname: Stender
authors.affiliation: Department of Human Nutrition, Centre for Advanced Food Studies, Faculty of Life Sciences, University of Copenhagen, Frederiksberg, Denmark; Department of Clinical Nutrition, Copenhagen University Hospital, Gentofte, Denmark.
authors.forename: Arne
authors.initials: A
authors.lastname: Astrup
mesh_terms: D000328:Adult; D001777:Blood Coagulation / Q000502:physiology*; D002318:Cardiovascular Diseases / Q000097:blood* / Q000517:prevention & control*; D018752:Diet, Fat-Restricted / Q000379:methods*; D004041:Dietary Fats / Q000008:administration & dosage*; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D005247:Feeding Behavior; D005260:Female; D006801:Humans; D008297:Male; D019518:Postprandial Period / Q000502:physiology*; D011446:Prospective Studies; D015431:Weight Loss
chemical_list: D004041:Dietary Fats; D004042:Dietary Fats, Unsaturated
keywords: Coagulation activation; Diet interventions; Haemostasis; MUFA; Postprandial