title: A convenient system for highly specific and sensitive detection of miRNA expression.
abstract: Since the first miRNA was discovered in 1993, miRNAs have become a hotspot for biological research. In order to feed this demand, a robust method is required to detect miRNA gene expression. Development of a detection method is more difficult for miRNAs than for long RNAs, such as mRNA, owing to their small size. Existing methods have limitations; thus, new methods are required. We describe a new system for detecting miRNA expression, which can distinguish miRNA from its precursor and has single-nucleotide resolution. It has single molecule and multiplex detection potential. It may be performed as a polymerase chain reaction (PCR) method, a blotting method, or a macroarray method according to the analyst's preference. This personalized system provides a convenient tool for the detection of miRNA gene expression.
authors.forename: Xiangqi
authors.initials: X
authors.lastname: Li
authors.forename: Minjie
authors.initials: M
authors.lastname: Ni
authors.forename: Chaobao
authors.initials: C
authors.lastname: Zhang
authors.forename: Wubin
authors.initials: W
authors.lastname: Ma
authors.forename: Yonglian
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D020869:Gene Expression Profiling / Q000379:methods*; D055029:Inverted Repeat Sequences; D057230:Limit of Detection; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D051381:Rats
chemical_list: D035683:MicroRNAs; D012322:RNA Precursors
keywords: PCR; avidin–biotin detection system; color development; liquid hybridization; miRNA; small RNA