title: Optimization of IC Separation Based on Isocratic-to-Gradient Retention Modeling in Combination with Sequential Searching or Evolutionary Algorithm.
abstract: GRADIENT ION CHROMATOGRAPHY WAS USED FOR THE SEPARATION OF EIGHT SUGARS: arabitol, cellobiose, fructose, fucose, lactulose, melibiose, N-acetyl-D-glucosamine, and raffinose. The separation method was optimized using a combination of simplex or genetic algorithm with the isocratic-to-gradient retention modeling. Both the simplex and genetic algorithms provided well separated chromatograms in a similar analysis time. However, the simplex methodology showed severe drawbacks when dealing with local minima. Thus the genetic algorithm methodology proved as a method of choice for gradient optimization in this case. All the calculated/predicted chromatograms were compared with the real sample data, showing more than a satisfactory agreement.
authors.affiliation: Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, Croatia.
authors.forename: Sime
authors.initials: S
authors.lastname: Ukić
authors.affiliation: Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, Croatia.
authors.forename: Marko
authors.initials: M
authors.lastname: Rogošić
authors.affiliation: Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, Croatia.
authors.forename: Mirjana
authors.initials: M
authors.lastname: Novak
authors.affiliation: Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, Croatia.
authors.forename: Ena
authors.initials: E
authors.lastname: Simović
authors.affiliation: Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, Croatia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Tišler
authors.affiliation: Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, Croatia.
authors.forename: Tomislav
authors.initials: T
authors.lastname: Bolanča