title: Protective effects of probiotic Lactobacillus rhamnosus IMC501 in mice treated with PhIP.
abstract: The aim of the present study was to investigate the antigenotoxic properties of the probiotic Lactobacillus rhamnosus IMC501; DNA damage was induced by one representative food mutagen, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). Mice were treated orally with suspensions of lactobacilli for 10 days before administration of food mutagen. During the treatment, the abundance of lactobacilli in feces, as assessed by qPCR analysis, increased, whereas β-glucuronidase and N-acetyl-β-glucosaminidase activities decreased. The extent of DNA damage was measured in colon and liver cells by comet assay. In colonocytes, diet supplementation with IMC501 resulted in a significant inhibition of DNA damage induced by PhIP. The results obtained in this in vitro study suggest that Lactobacillus rhamnosus IMC501 used as a dietary supplement can provide a useful integration of antimutagen food components of the normal diet, which are generally lower than the protective level.
authors.affiliation: Department of Pharmaceutical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, Italy, Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06122 Perugia, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Dominici
authors.forename: Milena
authors.initials: M
authors.lastname: Villarini
authors.forename: Francesca
authors.initials: F
authors.lastname: Trotta
authors.forename: Ermanno
authors.initials: E
authors.lastname: Federici
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cenci
authors.forename: Massimo
authors.initials: M
authors.lastname: Moretti
mesh_terms: D000118:Acetylglucosaminidase / Q000032:analysis; D000818:Animals; D058491:Bacterial Load; D002273:Carcinogens / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000633:toxicity*; D020552:Comet Assay; D019587:Dietary Supplements; D004847:Epithelial Cells / Q000187:drug effects; D005243:Feces / Q000201:enzymology / Q000382:microbiology; D005966:Glucuronidase / Q000032:analysis; D022781:Hepatocytes / Q000187:drug effects; D007093:Imidazoles / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000633:toxicity*; D052201:Lacticaseibacillus rhamnosus / Q000378:metabolism*; D051379:Mice; D019936:Probiotics / Q000008:administration & dosage*; D060888:Real-Time Polymerase Chain Reaction; D016896:Treatment Outcome
chemical_list: D002273:Carcinogens; D007093:Imidazoles; C049584:2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine; D005966:Glucuronidase; D000118:Acetylglucosaminidase