title: S-allylcysteine, a garlic derivative, suppresses proliferation and induces apoptosis in human ovarian cancer cells in vitro.
abstract: OBJECTIVE
  To investigate the effects of S-allylcysteine (SAC), a water-soluble garlic derivative, on human ovarian cancer cells in vitro.
  
  
  METHODS
  Human epithelial ovarian cancer cell line A2780 was tested. Cell proliferation was examined with CCK-8 and colony formation assays. Cell cycle was analyzed with flow cytometry. Cell apoptosis was studied using Hoechst 33258 staining and Annexin V/PI staining with flow cytometry. The migration and invasion of A2780 cells were examined with transwell and wound healing assays. The expression of relevant proteins was detected with Western blot assays.
  
  
  RESULTS
  SAC (1-100 mmol/L) inhibited the proliferation of A2780 cells in dose- and time-dependent manners (the IC50 value was approximately 25 mmol/L at 48 h, and less than 6.25 mmol/L at 96 h). Furthermore, SAC dose-dependently inhibited the colony formation of A2780 cells. Treatment of A2780 cells with SAC resulted in G1/S phase arrest and induced apoptosis, accompanied by decreased expression of pro-caspase-3, Parp-1 and Bcl-2, and increased expression of active caspase-3 and Bax. SAC treatment significantly reduced the migration of A2780 cells, and markedly decreased the protein expression of Wnt5a, p-AKT and c-Jun, which were the key proteins involved in proliferation and metastasis.
  
  
  CONCLUSIONS
  SAC suppresses proliferation and induces apoptosis in A2780 ovarian cancer cells in vitro.
authors.affiliation: 1] College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310022, China [2] Cancer Research Institute, Zhejiang Cancer Hospital, Hangzhou 310022, China.
authors.forename: Ya-si
authors.initials: YS
authors.lastname: Xu
authors.affiliation: Cancer Research Institute, Zhejiang Cancer Hospital, Hangzhou 310022, China.
authors.forename: Jian-guo
authors.initials: JG
authors.lastname: Feng
authors.affiliation: College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310022, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: 1] Laboratory of Clinical Pharmacy, Zhejiang Cancer Hospital, Hangzhou 310022, China [2] Cancer Research Institute, Zhejiang Cancer Hospital, Hangzhou 310022, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310022, China.
authors.forename: Min
authors.initials: M
authors.lastname: Luo
authors.affiliation: 1] Cancer Research Institute, Zhejiang Cancer Hospital, Hangzhou 310022, China [2] College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310022, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Su
authors.affiliation: 1] Laboratory of Clinical Pharmacy, Zhejiang Cancer Hospital, Hangzhou 310022, China [2] Cancer Research Institute, Zhejiang Cancer Hospital, Hangzhou 310022, China.
authors.forename: Neng-ming
authors.initials: NM
authors.lastname: Lin
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003545:Cysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D005737:Garlic / Q000378:metabolism*; D006801:Humans; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000378:metabolism
chemical_list: C065299:S-allylcysteine; D003545:Cysteine