title: Analysis of facial skin-resident microbiota in Japanese acne patients.
abstract: OBJECTIVE
  We investigated the facial skin microbiota of Japanese acne patients.
  
  
  METHODS
  Skin swab samples were obtained from 100 acne patients and 28 healthy controls to evaluate Propionibacterium and Staphylococcus spp. using a culture method. Malassezia spp. were evaluated using a nonculture method. Antibiotic resistance of Propionibacterium spp. was also examined.
  
  
  RESULTS
  Acne patients and controls did not show significant differences in Propionibacterium and Staphylococcus spp. populations. However, the number of Malassezia globosa from patients was greater than that from controls. Moreover, the number of Propionibacterium spp. from patients carrying antibiotic-resistant strains was significantly greater than that from patients not carrying them.
  
  
  CONCLUSIONS
  The present study characterized the facial skin microbiota of Japanese acne patients, suggesting a correlation between acne and quantitative differences in Malassezia microbiota. It was also found that the antibiotic resistance of Propionibacterium spp. may affect its abundance in the skin.
authors.affiliation: Department of Dermatology, Fujita Health University School of Medicine, Toyoake, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Numata
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Akamatsu
authors.forename: Narifumi
authors.initials: N
authors.lastname: Akaza
authors.forename: Akiko
authors.initials: A
authors.lastname: Yagami
authors.forename: Satoru
authors.initials: S
authors.lastname: Nakata
authors.forename: Kayoko
authors.initials: K
authors.lastname: Matsunaga
mesh_terms: D000152:Acne Vulgaris / Q000382:microbiology*; D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D024881:Drug Resistance, Bacterial; D005145:Face / Q000382:microbiology*; D005260:Female; D006801:Humans; D007564:Japan; D008290:Malassezia / Q000302:isolation & purification*; D008297:Male; D008826:Microbial Sensitivity Tests; D064307:Microbiota; D011424:Propionibacterium / Q000187:drug effects / Q000302:isolation & purification*; D012867:Skin / Q000382:microbiology*; D013210:Staphylococcus / Q000302:isolation & purification*; D055815:Young Adult