title: Acute respiratory distress syndrome (ARDS) in the course of influenza A/H1N1v infection--genetic aspects.
abstract: Influenza is a contagious respiratory disease caused by viruses belonging to the family Ortomyxoviridae. Among the influenza viruses type A, B and C, the A type virus shows the most pathogenic potential. Its surface receptor glycoproteins, hemagglutinin (HA) and neuraminidase (NA), are characterized by high antigenic variation, thus a host organism cannot develop permanent resistance. The case is described of a male patient with severe acute respiratory distress syndrome in the course of influenza A/N1H1v infection, confirmed by virological molecular analysis. During diagnostic procedures based on the MSSCP genotyping it was observed that the WHO recommended RT-PCR kits and/or procedure of sample collection from patients for molecular investigation could lead to false positive A/H1N1 pandemic strain detection because of the co-amplification during the RT-PCR fragments of the human genome.
authors.affiliation: Military Institute of Hygiene and Epidemiology, Biological Threat Identification and Countermeasures Centre, Pulawy, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Niemcewicz
authors.affiliation: BioVectis, Warsaw, Poland; Electron Microscopy Platform, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Pająk
authors.affiliation: Military Institute of Hygiene and Epidemiology, Biological Threat Identification and Countermeasures Centre, Pulawy, Poland.
authors.forename: Aleksander
authors.initials: A
authors.lastname: Michalski
authors.affiliation: Military Institute of Hygiene and Epidemiology, Biological Threat Identification and Countermeasures Centre, Pulawy, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Kocik
authors.affiliation: Military Institute of Hygiene and Epidemiology, Biological Threat Identification and Countermeasures Centre, Pulawy, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Kołodziej
authors.affiliation: Military Institute of Hygiene and Epidemiology, Biological Threat Identification and Countermeasures Centre, Pulawy, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Joniec
authors.affiliation: Military Institute of Hygiene and Epidemiology, Biological Threat Identification and Countermeasures Centre, Pulawy, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Graniak
authors.affiliation: Military Institute of Hygiene and Epidemiology, Biological Threat Identification and Countermeasures Centre, Pulawy, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Gaweł
authors.affiliation: Department of Expert Medical Assistance with Emergency Medicine Unit, Medical University, Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Marciniak-Niemcewicz
authors.affiliation: BioVectis, Warsaw, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Kucharczyk
authors.affiliation: Department of Internal Medicine, Independent Public Clinical Hospital, Lublin, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Prystupa
authors.affiliation: Department of Internal Medicine, Independent Public Clinical Hospital, Lublin, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Witczak
authors.affiliation: Military Institute of Hygiene and Epidemiology, Biological Threat Identification and Countermeasures Centre, Pulawy, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Lasocki
authors.affiliation: Department of Expert Medical Assistance with Emergency Medicine Unit, Medical University, Lublin, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Naylor
authors.affiliation: Department of Expert Medical Assistance with Emergency Medicine Unit, Medical University, Lublin, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Goniewicz
mesh_terms: D017809:Fatal Outcome; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics*; D007251:Influenza, Human / Q000150:complications* / Q000821:virology; D008297:Male; D008875:Middle Aged; D012128:Respiratory Distress Syndrome / Q000209:etiology*