title: CXCL10 decreases GP73 expression in hepatoma cells at the early stage of hepatitis C virus (HCV) infection.
abstract: Golgi protein 73 (GP73), which is up-regulated in hepatocellular carcinoma (HCC), has recently been identified as a novel serum marker for HCC diagnosis. Several reports also noted the increased levels of GP73 expression in chronic liver disease in patients with acute hepatitis of various etiologies, chronic Hepatitis C virus (HCV) infection and alcoholic liver disease. The molecular mechanisms of GP73 expression in HCV related liver disease still need to be determined. In this study, we aimed to evaluate the effect of HCV infection on GP73 expression. GP73 was highly expressed in Huh7, Hep3B, 293T and HUVEC cells, and was low-expressed in HepG2 cells. HCV infection led to down-regulation of GP73 in Huh7 and HepG2/CD81 cells at the early stage of infection. CXCL10 decreased GP73 expression in Huh7 and HepG2 cells. Up-regulation of GP73 was noted in hepatocytes with cytopathic effect at advanced stage of HCV infection, and further research is needed to determine the unknown factors affecting GP73 expression. In conclusion, our study provided additional evidence for the roles of GP73 in liver disease.
authors.affiliation: Department of Microbiology and Immunology, Center of Laboratory Medicine, General Hospital of Chengdu Military Region of PLA, Chengdu 610083, China. liuyuan198231@163.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Ziying
authors.initials: Z
authors.lastname: Zou
authors.forename: Bing
authors.initials: B
authors.lastname: Zhu
authors.forename: Zonghai
authors.initials: Z
authors.lastname: Hu
authors.forename: Ping
authors.initials: P
authors.lastname: Zeng
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000150:complications / Q000235:genetics* / Q000473:pathology; D002460:Cell Line; D049109:Cell Proliferation; D054357:Chemokine CXCL10 / Q000737:chemistry / Q000378:metabolism*; D015536:Down-Regulation; D057809:HEK293 Cells; D056945:Hep G2 Cells; D016174:Hepacivirus / Q000502:physiology; D006526:Hepatitis C / Q000150:complications* / Q000378:metabolism* / Q000473:pathology; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008113:Liver Neoplasms / Q000150:complications / Q000235:genetics* / Q000473:pathology; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D015854:Up-Regulation; D014779:Virus Replication
chemical_list: D054357:Chemokine CXCL10; C410824:GOLM1 protein, human; D008565:Membrane Proteins; D012333:RNA, Messenger