title: In vitro glucuronidation of ochratoxin a by rat liver microsomes.
abstract: Ochratoxin A (OTA), one of the most toxic mycotoxins, can contaminate a wide range of food and feedstuff. To date, the data on its conjugates via glucuronidation request clarification and consolidation. In the present study, the combined approaches of ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), UHPLC-Orbitrap-high resolution mass spectrometry (HRMS) and liquid chromatography-multiple stage mass spectrometry (LC-MS(n)) were utilized to investigate the metabolic profile of OTA in rat liver microsomes. Three conjugated products of OTA corresponding to amino-, phenol- and acyl-glucuronides were identified, and the related structures were confirmed by hydrolysis with β-glucuronidase. Moreover, OTA methyl ester, OTα and OTα-glucuronide were also found in the reaction solution. Based on these results, an in vitro metabolic pathway of OTA has been proposed for the first time.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Han
authors.forename: Emmanuel K
authors.initials: EK
authors.lastname: Tangni
authors.forename: José Diana
authors.initials: JD
authors.lastname: Di Mavungu
authors.forename: Lynn
authors.initials: L
authors.lastname: Vanhaecke
authors.forename: Sarah
authors.initials: S
authors.lastname: De Saeger
authors.affiliation: Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Shanghai 201403, China. wuaibo@saas.sh.cn.
authors.forename: Aibo
authors.initials: A
authors.lastname: Wu
authors.forename: Alfons
authors.initials: A
authors.lastname: Callebaut
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D020719:Glucuronides / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D009793:Ochratoxins / Q000378:metabolism*; D051381:Rats; D053719:Tandem Mass Spectrometry
chemical_list: D020719:Glucuronides; D009793:Ochratoxins; C025589:ochratoxin A