title: Fish cholinesterases as biomarkers of sublethal effects of organophosphorus and carbamates in tissues of Labeo rohita.
abstract: Organophosphates and carbamates are major agrochemicals that strongly affect different neuroenzymes and the growth of various fish species. Here, we study the effect of sublethal concentrations of profenofos and carbofuran on the activity of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) and the associated health risk in fish. Labeo rohita fingerlings were exposed to three sublethal concentrations of profenofos and carbofuran. The minimum cholinesterase activities in the brain, gills, muscle, kidney, liver, and blood were after exposure to profenofos (0.06 mg/L). The minimum AChE and BuChE activities in the brain, gills, muscle, kidney, liver, and blood were after exposure to carbofuran (0.28 and 0.198 mg/L). Exposure to both types of pesticides affected the functions of these organs, including metabolism and neurotransmission, to various extents at different exposure concentrations. These findings suggest that they are required to be properly monitored in the environment, to reduce their toxic effects on nontarget organisms.
authors.affiliation: Department of Environmental Sciences, Government College University, Faisalabad, Pakistan.
authors.lastname: Ghazala
authors.forename: Shahid
authors.initials: S
authors.lastname: Mahboob
authors.forename: L
authors.initials: L
authors.lastname: Ahmad
authors.forename: S
authors.initials: S
authors.lastname: Sultana
authors.forename: K
authors.initials: K
authors.lastname: Alghanim
authors.forename: F
authors.initials: F
authors.lastname: Al-Misned
authors.forename: Z
authors.initials: Z
authors.lastname: Ahmad
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000032:analysis* / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D002091:Butyrylcholinesterase / Q000378:metabolism*; D002219:Carbamates / Q000633:toxicity; D002235:Carbofuran / Q000633:toxicity*; D003530:Cyprinidae / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D063086:Organothiophosphates / Q000633:toxicity*; D010575:Pesticides / Q000633:toxicity; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D015415:Biomarkers; D002219:Carbamates; D063086:Organothiophosphates; D010575:Pesticides; D014874:Water Pollutants, Chemical; C024273:profenofos; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D002235:Carbofuran
keywords: Acetylcholine-sterase; Butyrylcholinesterase; Carbofuran; Inhibition; Profenofos