title: Towards lipidomics of low-abundant species for exploring tumor heterogeneity guided by high-resolution mass spectrometry imaging.
abstract: Many studies have evidenced the main role of lipids in physiological and also pathological processes such as cancer, diabetes or neurodegenerative diseases. The identification and the in situ localization of specific low-abundant lipid species involved in cancer biology are still challenging for both fundamental studies and lipid marker discovery. In this paper, we report the identification and the localization of specific isobaric minor phospholipids in human breast cancer xenografts by FTICR MALDI imaging supported by histochemistry. These potential candidates can be further confirmed by liquid chromatography coupled with electrospray mass spectrometry (LC-ESI-MS) after extraction from the region of interest defined by MALDI imaging. Finally, this study highlights the importance of characterizing the heterogeneous distribution of low-abundant lipid species, relevant in complex histological samples for biological purposes.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Cimino
authors.affiliation: Mass Spectrometry Laboratory, GIGA-R, Department of Chemistry, University of Liege, Liege 4000, Belgium. dcalli@bwh.harvard.edu.
authors.forename: David
authors.initials: D
authors.lastname: Calligaris
authors.forename: Johann
authors.initials: J
authors.lastname: Far
authors.forename: Delphine
authors.initials: D
authors.lastname: Debois
authors.forename: Silvia
authors.initials: S
authors.lastname: Blacher
authors.forename: Nor Eddine
authors.initials: NE
authors.lastname: Sounni
authors.forename: Agnès
authors.initials: A
authors.lastname: Noel
authors.affiliation: Mass Spectrometry Laboratory, GIGA-R, Department of Chemistry, University of Liege, Liege 4000, Belgium. e.depauw@ulg.ac.be.
authors.forename: Edwin
authors.initials: E
authors.lastname: De Pauw
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D008819:Mice, Nude; D010743:Phospholipids / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014183:Transplantation, Heterologous
chemical_list: D010743:Phospholipids