title: TNF-α regulates natriuretic peptides and aquaporins in human bronchial epithelial cells BEAS-2B.
abstract: Postoperative-fluid retention is a severe complication frequently reported in patients undergoing major surgical procedures. The complex network of molecules involved in such a severe surgery-induced condition remains poorly understood. Inflammation has been proposed among the various causes of fluid retention. Since TNF-α is one of the main proinflammatory cytokine initially released after major surgery, it is reasonable to assume its involvement in fluid overload. Here, we showed that TNF-α selectively regulates key molecules involved in fluids balance, such as natriuretic peptides (NPs) and aquaporins, in human bronchial epithelial cells BEAS-2B. In particular, we found that TNF-α induced a decrease of arial natriuretic peptide, natriuretic peptide receptor-1, aquaporin-1 and aquaporin-5 and an increase of brain natriuretic peptide with a different involvement of nuclear factor-κB and mitogen-activated protein kinases signaling pathway activation. Moreover, the observed changes in NPs expression, demonstrate inflammation as an additional cause of brain natriuretic peptide elevation, adding an important piece of information in the novel area of study regarding NPs and inflammation. Finally, we suggest that inflammation is one of the mechanisms of Aquaporin-1 and aquaporin-5 expression regulation. Therefore, in this exploratory study, we speculate that TNF-α might be involved in postoperative-fluid retention related to major surgery.
authors.affiliation: Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Polo Unico Sant'Andrea delle Fratte, 06156 Perugia, Italy.
authors.forename: Letizia
authors.initials: L
authors.lastname: Mezzasoma
authors.affiliation: Thoracic Surgery Unit, Ospedale S. Maria della Misericordia, University of Perugia, S. Andrea delle Fratte, 06156 Perugia, Italy.
authors.forename: Lucio
authors.initials: L
authors.lastname: Cagini
authors.affiliation: Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Polo Unico Sant'Andrea delle Fratte, 06156 Perugia, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Antognelli
authors.affiliation: Thoracic Surgery Unit, Ospedale S. Maria della Misericordia, University of Perugia, S. Andrea delle Fratte, 06156 Perugia, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Puma
authors.affiliation: Clinical Pathology and Hematology Unit, Ospedale S. Maria della Misericordia of Perugia, S. Andrea delle Fratte, 06156 Perugia, Italy.
authors.forename: Eugenio
authors.initials: E
authors.lastname: Pacifico
authors.affiliation: Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Polo Unico Sant'Andrea delle Fratte, 06156 Perugia, Italy.
authors.forename: Vincenzo Nicola
authors.initials: VN
authors.lastname: Talesa
mesh_terms: D051398:Aquaporin 1 / Q000378:metabolism; D051402:Aquaporin 5 / Q000378:metabolism; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D001980:Bronchi / Q000166:cytology; D002460:Cell Line; D002470:Cell Survival; D003470:Culture Media / Q000737:chemistry; D003907:Dexamethasone / Q000737:chemistry; D004847:Epithelial Cells / Q000166:cytology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006439:Hemodynamics; D006706:Homeostasis; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D020097:Natriuretic Peptide, Brain / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D017461:Receptors, Atrial Natriuretic Factor / Q000378:metabolism*; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: C496020:AQP1 protein, human; C496036:AQP5 protein, human; D051402:Aquaporin 5; D003470:Culture Media; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D020097:Natriuretic Peptide, Brain; D051398:Aquaporin 1; D003907:Dexamethasone; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor; C091715:atrial natriuretic factor receptor A