title: Sphaerisporangium rufum sp. nov., an endophytic actinomycete from roots of Oryza sativa L.
abstract: An endophytic actinomycete, strain R10-82(T), isolated from surface-sterilized roots of rice (Oryza sativa L.) was studied using a polyphasic approach. Strain R10-82(T) produced branching substrate mycelia and developed spherical spore vesicles on aerial hyphae containing non-motile spores. The major cellular fatty acids were iso-C16 : 0, iso-C14 : 0 and 10-methyl C17 : 0. The predominant menaquinones were MK-9, MK-9(H2), MK-9(H4) and MK-9(H6). Rhamnose, ribose, madurose, mannose and glucose were detected in whole-cell hydrolysates. The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannosides, hydroxylphosphatidylethanolamine and ninhydrin-positive phosphoglycolipids. These morphological and chemotaxonomic data were similar to those of the genus Sphaerisporangium. Analysis of the 16S rRNA gene sequence revealed that strain R10-82(T) was related most closely to Sphaerisporangium cinnabarinum JCM 3291(T) (98.3 % similarity). The DNA G+C content of strain R10-82(T) was 74 mol%. DNA-DNA relatedness data in combination with differences in the biochemical and physiological properties suggested that strain R10-82(T) should be classified as representing a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium rufum is proposed. The type strain is R10-82(T) ( = BCC 51287(T) = NBRC 109079(T)). An emended description of the genus Sphaerisporangium is also provided.
authors.affiliation: Center for Advanced Studies in Tropical Natural Resources, NRU-KU, Kasetsart University, Chatuchak, Bangkok, Thailand.|Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: Ratchanee
authors.initials: R
authors.lastname: Mingma
authors.affiliation: Center for Advanced Studies in Tropical Natural Resources, NRU-KU, Kasetsart University, Chatuchak, Bangkok, Thailand.|Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: Kannika
authors.initials: K
authors.lastname: Duangmal
authors.affiliation: Center for Advanced Studies in Tropical Natural Resources, NRU-KU, Kasetsart University, Chatuchak, Bangkok, Thailand.|Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: Savitr
authors.initials: S
authors.lastname: Trakulnaleamsai
authors.affiliation: Department of Genetics, Faculty of Science, Kasetsart University, Bangkok, Thailand.|Center for Advanced Studies in Tropical Natural Resources, NRU-KU, Kasetsart University, Chatuchak, Bangkok, Thailand.
authors.forename: Arinthip
authors.initials: A
authors.lastname: Thamchaipenet
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Matsumoto
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Takahashi
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012275:Oryza / Q000382:microbiology*; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013785:Thailand; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9