title: A new theraphosid spider toxin causes early insect cell death by necrosis when expressed in vitro during recombinant baculovirus infection.
abstract: Baculoviruses are the most studied insect viruses in the world and are used for biological control of agricultural and forest insect pests. They are also used as versatile vectors for expression of heterologous proteins. One of the major problems of their use as biopesticides is their slow speed to kill insects. Thus, to address this shortcoming, insect-specific neurotoxins from arachnids have been introduced into the baculovirus genome solely aiming to improve its virulence. In this work, an insecticide-like toxin gene was obtained from a cDNA derived from the venom glands of the theraphosid spider Brachypelma albiceps. The mature form of the peptide toxin (called Ba3) has a high content of basic amino acid residues, potential for three possible disulfide bonds, and a predicted three-stranded β-sheetDifferent constructions of the gene were engineered for recombinant baculovirus Autographa californica multiple nuclepolyhedrovirus (AcMNPV) expression. Five different forms of Ba3 were assessed; (1) the full-length sequence, (2) the pro-peptide and mature region, (3) only the mature region, and the mature region fused to an (4) insect or a (5) virus-derived signal peptide were inserted separately into the genome of the baculovirus. All the recombinant viruses induced cell death by necrosis earlier in infection relative to a control virus lacking the toxin gene. However, the recombinant virus containing the mature portion of the toxin gene induced a faster cell death than the other recombinants. We found that the toxin construct with the signal peptide and/or pro-peptide regions delayed the necrosis phenotype. When infected cells were subjected to ultrastructural analysis, the cells showed loss of plasma membrane integrity and structural changes in mitochondria before death. Our results suggest this use of baculovirus is a potential tool to help understand or to identify the effect of insect-specific toxic peptides when produced during infection of insect cells.
authors.affiliation: Departmento de Biologia Celular, Universidade de Brasília, Brasília, Brasília, DF, Brazil.
authors.forename: Daniel Mendes Pereira
authors.initials: DM
authors.lastname: Ardisson-Araújo
authors.affiliation: Departmento de Biologia Celular, Universidade de Brasília, Brasília, Brasília, DF, Brazil.
authors.forename: Fabrício Da Silva
authors.initials: Fda S
authors.lastname: Morgado
authors.affiliation: Departmento de Ciêncais Fisiológicas, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Elisabeth Ferroni
authors.initials: EF
authors.lastname: Schwartz
authors.affiliation: Departamento de Medicina Molecular y Bioprocesos, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Corzo
authors.affiliation: Departmento de Biologia Celular, Universidade de Brasília, Brasília, Brasília, DF, Brazil.
authors.forename: Bergmann Morais
authors.initials: BM
authors.lastname: Ribeiro
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000096:biosynthesis* / Q000235:genetics; D002460:Cell Line; D009336:Necrosis / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D017924:Nucleopolyhedroviruses; D013111:Spider Venoms / Q000096:biosynthesis* / Q000235:genetics; D013112:Spiders / Q000235:genetics*; D018411:Spodoptera
chemical_list: D060829:Arthropod Proteins; D013111:Spider Venoms