title: [Reversed-phase high performance liquid chromatography separation of 2-( fluorophenyl) -5-methylbenzoxazole and 2-( chlorophenyl) -5-methylbenzoxazole positional isomers].
abstract: The analytical method of 2-( fluorophenyl)-5-methylbenzoxazole ortho-, meta-, para-positional isomers and 2-( chlorophenyl) -5-methylbenzoxazole ortho-, meta-, para-positional isomers was developed with a commercial common liquid chromatography column. The separation was performed on an Inertsil ODS-SP C18 column (250 mm x 4.6 mm, 5 microm) at 40 degrees C with a linear gradient elution by mobile phases of acetonitrile (A) (from 60% to 80% within 15 min) and water (B) at a flow rate of 1.5 mL/min. The samples were detected by a diode array detector at 310 nm. Good linearities were obtained in the range of 2 - 200 mg/L for the six isomers. The limits of detection (LODs) (S/N = 3) of 2- (fluorophenyl) -5-methylbenzoxazole ortho-, meta-, para-isomers and 2-( chlorophenyl)-5-methylbenzoxazole ortho-, meta-, para-isomers were 0.0307, 0.0293, 0.0315, 0.0226, 0.0237, 0.0226 mg/L, respectively. The method provides not only a rapid detection method for the preparation of the isomers through hydrocarbon activation coupling reaction between the 5-methylbenzoxazole and fluorobenzene or chlorobenzene but also a reference for the separation and detection of 2-arylbenzoxazole isomers.
authors.affiliation: Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China. wangmin04@fjirsm.ac.cn
authors.forename: Min
authors.initials: M
authors.lastname: Wang