title: Ultrastructural study of the kidney subjected to warm ischemia and perfused with inosine.
abstract: OBJECTIVE
  Renal ischemia, depending on its duration, results in organ function loss to a greater or lesser extent, due to the depletion of the energy cells need for their vital functions. The method of supplying an external energy source that may act as a precursor of ATP, such as inosine, has proved to be protective from a functional point of view. In this work, we aim to reveal the histological ultrastructural bases that underlie this protective effect.
  
  
  METHODS
  We studied two groups of rats subjecting the kidneys to different durations of warm ischemia, and compared the histological findings at various parts of the nephron after perfusion with saline or inosine. These findings were compared, in turn, with the normal morphology of a third control group.
  
  
  RESULTS
  The histological findings were: 1. No significant lesions after 60 and 120 min of warm ischemia in animals perfused with inosine; and 2. Glomerular and tubular injury after 60 min of warm ischemia in animals perfused with saline.
  
  
  CONCLUSIONS
  The saline-perfused animals showed very significant injury at the glomerular and tubular levels after 60 and 120 min of warm ischemia. These lesions were not seen in animals perfused with inosine. The similarity of the morphological findings between the inosine-infused group and the control group suggests that inosine has a protective effect on the morphology of the rat nephron under conditions of warm ischemia for periods shorter than 120 min.
authors.affiliation: Servicio de Urologia. Hospital Universitario de Leon. Departamento de Anatomia Patologica e Histologia Humanas. Facultad de Medicina. Universidad de Salamanca.Spain.
authors.forename: F J
authors.initials: FJ
authors.lastname: Gallo  Rolanía
authors.forename: A J
authors.initials: AJ
authors.lastname: Álvarez-Morujo Suarez
authors.forename: F P
authors.initials: FP
authors.lastname: Collía Fernandez
mesh_terms: D000818:Animals; D007288:Inosine; D007511:Ischemia; D007668:Kidney; D010477:Perfusion; D052096:Warm Ischemia
chemical_list: D007288:Inosine