title: Optical imaging of fibrin deposition to elucidate participation of mast cells in foreign body responses.
abstract: Mast cell activation has been shown to be an initiator and a key determinant of foreign body reactions. However, there is no non-invasive method that can quantify the degree of implant-associated mast cell activation. Taking advantage of the fact that fibrin deposition is a hallmark of mast cell activation around biomaterial implants, a near infrared probe was fabricated to have high affinity to fibrin. Subsequent in vitro testing confirmed that this probe has high affinity to fibrin. Using a subcutaneous particle implantation model, we found significant accumulation of fibrin-affinity probes at the implant sites as early as 15 min following particle implantation. The accumulation of fibrin-affinity probes at the implantation sites could also be substantially reduced if anti-coagulant - heparin was administered at the implant sites. Further studies have shown that subcutaneous administration of mast cell activator - compound 48/80 - prompted the accumulation of fibrin-affinity probes. However, implant-associated fibrin-affinity probe accumulation was substantially reduced in mice with mast cell deficiency. The results show that our fibrin-affinity probes may serve as a powerful tool to monitor and measure the extent of biomaterial-mediated fibrin deposition and mast cell activation in vivo.
authors.affiliation: Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76019, USA.
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Tsai
authors.affiliation: Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76019, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76019, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Weng
authors.affiliation: Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76019, USA.
authors.forename: Ewin N
authors.initials: EN
authors.lastname: Tang
authors.affiliation: Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76019, USA.
authors.forename: David W
authors.initials: DW
authors.lastname: Baker
authors.affiliation: Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76019, USA.|Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Liping
authors.initials: L
authors.lastname: Tang
mesh_terms: D000818:Animals; D005260:Female; D005337:Fibrin / Q000378:metabolism*; D005549:Foreign-Body Reaction / Q000378:metabolism / Q000503:physiopathology*; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D005337:Fibrin
keywords: Biocompatibility; Biomaterial; Fibrin; Inflammation; In vivo imaging; Near infrared fluorescence