title: PVv3, a new shuttle vector for gene expression in Vibrio vulnificus.
abstract: An efficient electroporation procedure for Vibrio vulnificus was designed using the new cloning vector pVv3 (3,107 bp). Transformation efficiencies up to 2 × 10(6) transformants per μg DNA were achieved. The vector stably replicated in both V. vulnificus and Escherichia coli and was also successfully introduced into Vibrio parahaemolyticus and Vibrio cholerae. To demonstrate the suitability of the vector for molecular cloning, the green fluorescent protein (GFP) gene and the vvhBA hemolysin operon were inserted into the vector and functionally expressed in Vibrio and E. coli.
authors.affiliation: Bundesinstitut für Risikobewertung, Berlin, Germany.
authors.forename: Karina
authors.initials: K
authors.lastname: Klevanskaa
authors.forename: Nadja
authors.initials: N
authors.lastname: Bier
authors.forename: Kerstin
authors.initials: K
authors.lastname: Stingl
authors.forename: Eckhard
authors.initials: E
authors.lastname: Strauch
authors.forename: Stefan
authors.initials: S
authors.lastname: Hertwig
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D018274:Electroporation / Q000379:methods; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D017930:Genes, Reporter; D005822:Genetic Vectors; D042822:Genomic Instability; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014169:Transformation, Bacterial; D014734:Vibrio cholerae / Q000235:genetics; D014736:Vibrio parahaemolyticus / Q000235:genetics; D041261:Vibrio vulnificus / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C470630:VvhA protein, Vibrio vulnificus; D049452:Green Fluorescent Proteins