title: Histological and gene expression analysis of the effects of pulsed low-level laser therapy on wound healing of streptozotocin-induced diabetic rats.
abstract: Diabetes mellitus (DM) is associated with poor wound healing. Studies have shown accelerated wound healing following pulsed low-level laser therapy (LLLT) in non-diabetic animals. The present study aims to evaluate the effect of pulsed LLLT on wound healing in streptozotocin-induced diabetic (STZ-D) rats. We divided 48 rats into two groups of non-diabetic and diabetic. Type 1 DM was induced in the diabetic rat group by injections of STZ. Two, full-thickness skin incisions were made on the dorsal region of each rat. One month after the STZ injection, wounds of the non-diabetic and diabetic rats were submitted to a pulsed, infrared 890-nm laser with an 80-Hz frequency and 0.2 J/cm(2) for each wound point. Control wounds did not receive LLLT. Animals were sacrificed on days 4, 7, and 15 post-injury for histomorphometry and reverse transcription polymerase chain reaction (RT-PCR) analyses of basic fibroblast growth factor (bFGF) gene expression. Pulsed LLLT significantly increased the numbers of macrophages, fibroblasts, and blood vessel sections compared to the corresponding control groups. Semi-quantitative analysis of bFGF gene expression at 48 h post-injury revealed a significant increase in gene expression in both non-diabetic and diabetic rats following LLLT (the ANOVA test). Pulsed LLLT at 0.2 J/cm(2) accelerated the wound healing process in both non-diabetic and diabetic rats as measured by histological characteristics and semi-quantitative bFGF gene expression.
authors.affiliation: Department of Anatomy, Medical Faculty, Isfahan University of Medical Sciences, Isfahan, Iran, vahidsharif@yahoo.com.
authors.forename: Zanelabedien
authors.initials: Z
authors.lastname: Sharifian
authors.forename: Mohammad
authors.initials: M
authors.lastname: Bayat
authors.forename: Morteza
authors.initials: M
authors.lastname: Alidoust
authors.forename: Reza Masteri
authors.initials: RM
authors.lastname: Farahani
authors.forename: Maryam
authors.initials: M
authors.lastname: Bayat
authors.forename: Fatemealsadat
authors.initials: F
authors.lastname: Rezaie
authors.forename: Homa
authors.initials: H
authors.lastname: Bayat
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy*; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics / Q000473:pathology / Q000532:radiotherapy; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005786:Gene Expression Regulation; D028022:Low-Level Light Therapy; D008297:Male; D017208:Rats, Wistar; D013311:Streptozocin; D014945:Wound Healing / Q000235:genetics* / Q000528:radiation effects*
chemical_list: D016222:Fibroblast Growth Factor 2; D013311:Streptozocin