title: Effect of pre-analytical conditions on the thrombodynamics assay.
abstract: BACKGROUND
  Standardization of pre-analytical conditions is the obligatory step for all potential diagnostic tests. Spatial clot growth (Thrombodynamics) is a new global hemostasis assay that considers spatial organization of coagulation. The principal parameter is rate of fibrin clot growth from the tissue-factor coated surface. In this work we studied the pre-analytical variables of Thrombodynamics assay that include conditions of blood collection, sample preparation and storage.
  
  
  METHODS
  Blood of apparently healthy volunteers was used. Eight types of citrate blood collection tubes were tested, centrifugation conditions for plasma preparation were evaluated and impact of plasma freezing/thawing was tested.
  
  
  RESULTS
  Among the blood collection tubes tested, BD Vacutainer glass tubes showed a significantly higher clot growth rate compared to plastic tubes. There was no difference between 3.2% and 3.8% of sodium citrate. For plasma preparation, a single 15 min centrifugation at 1,600 g shows significantly increased clot growth rate compared to plasma obtained by two sequential centrifugations (15 min 1 600 g, 5min 10,000 g). There was no significant difference between 1,600 g and 2,100 g if the second centrifugation was performed. For the second centrifugation there was no difference between 20 min at 1 600 g and 5 min at 10,000g. Frozen-thawed plasma showed increased clot growth rate compared to fresh plasma.
  
  
  CONCLUSIONS
  The data represent the necessary steps for the standardization of Thrombodynamics assay and for the formulation of the operating guide.
authors.affiliation: EA 4174, Université Lyon 1, Lyon, France; HemaCore LLC, Moscow, Russia; Center for Theoretical Problems of Physico-Chemical Pharmacology, Moscow, Russia,. Electronic address: dashkevichnm@gmail.com.
authors.forename: Natalia M
authors.initials: NM
authors.lastname: Dashkevich
authors.affiliation: Federal Research and Clinical Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia.
authors.forename: Tatiana A
authors.initials: TA
authors.lastname: Vuimo
authors.affiliation: Federal Research and Clinical Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia.
authors.forename: Ruzanna A
authors.initials: RA
authors.lastname: Ovsepyan
authors.affiliation: Federal Research and Clinical Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia.
authors.forename: Stepan S
authors.initials: SS
authors.lastname: Surov
authors.affiliation: HemaCore LLC, Moscow, Russia.
authors.forename: Natalia P
authors.initials: NP
authors.lastname: Soshitova
authors.affiliation: HemaCore LLC, Moscow, Russia; Center for Theoretical Problems of Physico-Chemical Pharmacology, Moscow, Russia,; National Research Center for Hematology, Moscow, Russia; Physics Department, Moscow State University, Moscow, Russia.
authors.forename: Mikhail A
authors.initials: MA
authors.lastname: Panteleev
authors.affiliation: HemaCore LLC, Moscow, Russia; Center for Theoretical Problems of Physico-Chemical Pharmacology, Moscow, Russia,; Federal Research and Clinical Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia; National Research Center for Hematology, Moscow, Russia; Physics Department, Moscow State University, Moscow, Russia.
authors.forename: Fazoil I
authors.initials: FI
authors.lastname: Ataullakhanov
authors.affiliation: EA 4174, Université Lyon 1, Lyon, France; Unite d'Hemostase Clinique, Hopital Edouard Herriot, Lyon, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Negrier
mesh_terms: D000328:Adult; D000368:Aged; D001777:Blood Coagulation / Q000502:physiology*; D001780:Blood Coagulation Tests / Q000379:methods*; D001800:Blood Specimen Collection / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation / Q000502:physiology; D013927:Thrombosis / Q000097:blood*; D055815:Young Adult
keywords: Coagulation tests; pre-analytical variables; thrombodynamics