title: ENV7 and YCK3, which encode vacuolar membrane protein kinases, genetically interact to impact cell fitness and vacuole morphology.
abstract: Saccharomyces cerevisiae vacuoles serve as a model for membrane fusion and fission. Yck3, a vacuolar membrane kinase, has been implicated in regulation of vacuole fusion. Recently, we established Env7 as another vacuolar membrane protein kinase with similar but nonredundant function to Yck3. Here, we report that native Env7 localizes to the vacuole independent of Yck3, where as its phosphorylation is YCK3 dependent. We also show that env7Δyck3Δ double mutant exhibits severely compromised fitness, altered cell size and bud vacuoles, and F-class vacuolar morphology. Our results establish negative genetic interactions between ENV7 and YCK3 and suggest cooperative roles for the two conserved genes in regulation of membrane dynamics. Such genetic buffering supports a critical role for membrane flux in global cell fitness.
authors.affiliation: California State University Long Beach (CSULB), Long Beach, CA, USA.
authors.forename: Surya P
authors.initials: SP
authors.lastname: Manandhar
authors.forename: Editte
authors.initials: E
authors.lastname: Gharakhanian
mesh_terms: D047389:Casein Kinase I / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000502:physiology* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014617:Vacuoles / Q000201:enzymology* / Q000648:ultrastructure*
chemical_list: D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D011494:Protein Kinases; D047389:Casein Kinase I; C000593862:Env7 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases; C479667:YCK3 protein, S cerevisiae
keywords: Env7; Yck3; endomembrane flux; vacuole biogenesis; vacuole fusion