title: Enantioselective determination of triazole fungicide epoxiconazole bioaccumulation in tubifex based on HPLC-MS/MS.
abstract: In this study, the enantioselective bioaccumulation of epoxiconazole enantiomers in tubifex (Oligochaeta, Tubificida) was investigated in two uptake pathways. A sensitive and rapid chiral method was developed for the determination of epoxiconazole enantiomers in tubifex and soil based on high-performance liquid chromatography coupled with triple-quadrupole mass spectrometry (HPLC-MS/MS). In the spiked-water or spiked-soil treatments, enantioselective bioaccumulation of epoxiconazole in tubifex was obersved. For spiked-water treatment, (-)-epoxiconazole accumulated in tubifex to a greater extent than (+)-epoxiconazole, leading to enrichments with a composition (-) > (+). However, for spiked-soil treatment, the enantioselectivity in tubifex was reversed with a preferential accumulation of (+)-epoxiconazole. Calculated accumulation factors (AFs) indicated that epoxiconazole in spiked-water treatment had higher bioaccumulation potential than that in spiked-soil treatment. The results from the spiked-soil treatment also revealed that the dissipation of epoxiconazole in soil was enantioselective, and tubifex has positive effects on epoxiconazole diffusion from soil to overlying water.
authors.affiliation: Department of Applied Chemistry, China Agricultural University , Yuanmingyuan West Road 2, Beijing 100193, China.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.forename: Tiantian
authors.initials: T
authors.lastname: Liu
authors.forename: Shanshan
authors.initials: S
authors.lastname: Di
authors.forename: Jinling
authors.initials: J
authors.lastname: Diao
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004852:Epoxy Compounds / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D005659:Fungicides, Industrial; D009835:Oligochaeta / Q000737:chemistry / Q000378:metabolism*; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000737:chemistry; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D053719:Tandem Mass Spectrometry; D014230:Triazoles / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D014867:Water / Q000737:chemistry
chemical_list: D004852:Epoxy Compounds; D005659:Fungicides, Industrial; D012987:Soil; D012989:Soil Pollutants; D014230:Triazoles; D014867:Water; C109476:epoxiconazole
keywords: bioaccumulation; enantioselectivity; epoxiconazole; tubifex