title: Changes in antioxidant activity during the ripening of jujube (Ziziphus mauritiana Lamk).
abstract: Phenolic compounds from jujube fruits and related antioxidant activities were investigated during the ripening stages. Three different antioxidant assays, including ORAC, FRAP and DPPH, were monitored on crude jujube extract (CJE). Jujube fruits were additionally fractionated into three selective fractions F1, F2, and F3. However, only the FRAP assay gave the relative antioxidant activity for the three fractions. Furthermore, HPLC-ESI-MSMS (Q-Tof) and GC-MS were used to identify the compounds in each purified fraction. Using FRAP, F1 mainly composed of lipids, exhibited the lowest antioxidant activity (≈0.080±0.015mmol trolox/100g, p<0.05). F2, rich in flavanols and flavonols, displayed 50-fold higher activity (4.27±0.11mmol trolox/100g). Remarkably, F3 with an elevated content of condensed tannins (polymeric proanthodelphinidins), exhibited the highest antioxidant activity (25.4±0.35mmol trolox/100g). The presented results showed that the phenolic profiles of the fruits were influenced by their developmental stage. Furthermore, during ripening, the antioxidant activity may be more impacted by the flavanols and condensed tannins. The purified condensed tannins of jujube fruits may be used as natural antioxidant extracts.
authors.affiliation: CIRAD, UMR QUALISUD, F-97130 Capesterre-Belle-Eau, Guadeloupe; CIRAD, UMR QUALISUD, F-34398 Montpellier, France.
authors.forename: Suzie
authors.initials: S
authors.lastname: Zozio
authors.affiliation: CIRAD, UMR QUALISUD, F-34398 Montpellier, France.
authors.forename: Adrien
authors.initials: A
authors.lastname: Servent
authors.affiliation: UNIVERSITE MONTPELLIER II, F-34095 Montpellier, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Cazal
authors.affiliation: CIRAD, UMR QUALISUD, F-97130 Capesterre-Belle-Eau, Guadeloupe; CIRAD, UMR QUALISUD, F-34398 Montpellier, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Mbéguié-A-Mbéguié
authors.affiliation: UNIVERSITÉ DES ANTILLES GUYANE (UAG), F-97157 Pointe à Pitre, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Ravion
authors.affiliation: CIRAD, UMR QUALISUD, F-34398 Montpellier, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Pallet
authors.affiliation: UNIVERSITÉ DES ANTILLES GUYANE (UAG), F-97157 Pointe à Pitre, France. Electronic address: abel.hiol@univ-reunion.fr.
authors.forename: Hiol
authors.initials: H
authors.lastname: Abel
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D044948:Flavonols / Q000032:analysis / Q000378:metabolism; D005638:Fruit / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism; D013058:Mass Spectrometry; D010636:Phenols / Q000032:analysis / Q000378:metabolism; D010936:Plant Extracts / Q000032:analysis* / Q000378:metabolism; D044945:Proanthocyanidins / Q000032:analysis / Q000378:metabolism; D031957:Ziziphus / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism
chemical_list: D000975:Antioxidants; D044948:Flavonols; D010636:Phenols; D010936:Plant Extracts; D044945:Proanthocyanidins
keywords: 2,2-diphenyl-1-picrylhydrazyl; Antioxidant activity; C; CJE; Condensed tannins; Cyan; DPPH; Dph; EC; EC(50); EPG; F1; F2; F3; FRAP; Flavanols; GC; GC–MS; GC–MS analysis; HPLC–ESI-MSMS (Q-Tof); Jujube fruits; LC–MS; ORAC; Ripening stages; Thiolysis; catechin; crude jujube extract; cyanidin; delphinidin; effective concentration; epicatechin; epigallocatechin; ferric reducing antioxidant power; fraction 1; fraction 2; fraction 3; gallocatechin; gas chromatography–mass spectrometry; high performance liquid chromatography–electrospray ionisation-tandem mass spectrometry (quadrupole-time-of-flight); liquid chromatography–mass spectrometry; mDP; mean degree of polymerisation; oxygen radical absorbance capacity