title: Where are signal molecules likely to be located in anaerobic granular sludge?
abstract: Quorum sensing is a concentration-sensing mechanism that plays a vital role in sludge granulation. In this study, the regularities of distribution of different signal molecules, including intra- and interspecific signal molecules (diffusible signal factor, DSF), interspecific signal molecules (autoinducter-2, AI-2) and intraspecific signal molecules (acyl-homoserine lactones, AHLs), from three types of anaerobic granular sludge were investigated. The results showed that 70-90% of DSF was distributed in sludge, while AI-2 in the Water phase accounted for over 80% of the total content. Interestingly, there was a positive correlation between DSF and AI-2, which played opposite roles in granulation. Moreover, more than 55% of short and medium acyl chain AHLs tended to spread in aqueous water, while the long acyl chain AHLs were closer to granular sludge than the short and medium acyl chain AHLs. With the exception of one type of sludge, the percentage of long acyl chain AHLs in the sludge phase was greater than 70%. The different distributions of signal molecules were primarily determined based on their physicochemical properties, including molecular weight and solubility in water or organic solutions. In addition, the basic properties of sludge, such as the granular level or the production of EPS, were closely related to the diversity, distribution and concentration of signal molecules. As a medium in granulation, extracellular polymeric substances production was regulated by different signal molecules from different parts of anaerobic granular sludge. This study provides a foundation for investigation of quorum sensing in the system of anaerobic granular sludge.
authors.affiliation: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China; Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou 310012, China.
authors.forename: Huajun
authors.initials: H
authors.lastname: Feng
authors.affiliation: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China; Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou 310012, China.
authors.forename: Yangcheng
authors.initials: Y
authors.lastname: Ding
authors.affiliation: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China; Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou 310012, China.
authors.forename: Meizhen
authors.initials: M
authors.lastname: Wang
authors.affiliation: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China.
authors.forename: Guanglan
authors.initials: G
authors.lastname: Zhou
authors.affiliation: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China; Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou 310012, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zheng
authors.affiliation: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China; Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou 310012, China.
authors.forename: Hongzhen
authors.initials: H
authors.lastname: He
authors.affiliation: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China; Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou 310012, China.
authors.forename: Xueqin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China; Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou 310012, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Shen
authors.affiliation: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China; Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou 310012, China. Electronic address: shentujl@mail.zjgsu.edu.cn.
authors.forename: Jiali
authors.initials: J
authors.lastname: Shentu
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism; D000693:Anaerobiosis; D055598:Chemical Phenomena; D007211:Indoles / Q000378:metabolism; D053038:Quorum Sensing; D012722:Sewage / Q000382:microbiology*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D054742:Acyl-Butyrolactones; D007211:Indoles; D012722:Sewage; D001616:beta-Galactosidase; C063155:violacein
keywords: Anaerobic granular sludge; Distribution; Quorum sensing; Signal molecules