title: Novel evolutionary models and periodic charts in p- and q-individual chromosomes of auxiliary lymph node and buccal cells.
abstract: Signal copy number (SCN) and signal intensity (SI) of subtelomeres (ST) are investigated in auxiliary lymph node (ALN) and buccal (BUC) cells by fluorescence in situ hybridization. The extracted total cell of 38256 and 2309 was, respectively, analyzed from the benign ALN- and BUC-cells of an affected breast cancer patient. The Periodic model was based on ST behavior including normal-, down-, and upregulated clones with diverse SCN. The arm-p/q ratio based signature, as a subtelomeric array, reflects discordance and concordance of ST-behavior within individual chromosomes as a concept of "Individualization of Cells" rather than "Global Insight of Cells". The Periodic charts could be considered as a reliable and refreshable platform through which the cellular evolution could be patterned and characterized. Signature of ST-profile in the BUC and ALN cells and the nature of diverse SCN and SI as quantitative and qualitative value led to modeling the real personalized perspective of cellular evolution. Protein expression of Ki67, Cyclin D1, and Cyclin E was assayed, as a complementary panel. These targets could be applied as the predictive and preventive markers for an early detection at BUC and ALN levels to plan the required managements in the breast cancer patients.
authors.affiliation: Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, P.O. Box 14176-13151, Tehran, Iran.
authors.forename: P
authors.initials: P
authors.lastname: Mehdipour
authors.affiliation: Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, P.O. Box 14176-13151, Tehran, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Javan
authors.affiliation: Department of Surgery, Day General Hospital, Tehran, Iran ; Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Atri
mesh_terms: D000368:Aged; D001943:Breast Neoplasms / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D002877:Chromosomes, Human / Q000235:genetics / Q000378:metabolism*; D019938:Cyclin D1 / Q000378:metabolism; D019927:Cyclin E / Q000378:metabolism; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D019394:Ki-67 Antigen / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology*; D008954:Models, Biological; D009061:Mouth Mucosa / Q000473:pathology*; D016615:Telomere / Q000235:genetics* / Q000378:metabolism
chemical_list: C530104:CCND1 protein, human; D019927:Cyclin E; D019394:Ki-67 Antigen; D019938:Cyclin D1