title: Genomic regions harboring insecticide resistance-associated Cyp genes are enriched by transposable element fragments carrying putative transcription factor binding sites in two sibling Drosophila species.
abstract: In the present study, an in silico analysis was performed to identify transposable element (TE) fragments inserted in Cyps with functions associated with resistance to insecticides and developmental regulation as well as in neighboring genes in two sibling species, Drosophila melanogaster and Drosophila simulans. The Cyps associated with insecticide resistance and their neighboring non-Cyp genes have accumulated a greater number of TE fragments than the other Cyps or a random sample of genes, predominantly in the 5'-flanking regions. Most of the insertions were due to DNA transposons, with DNAREP1 fragments being the most common. These fragments carry putative binding sites for transcription factors, which reinforces the hypothesis that DNAREP1 may influence gene regulation and play a role in the adaptation of the Drosophila species.
authors.affiliation: UNESP-Univ. Estadual Paulista, Departamento de Biologia, Laboratório de Evolução Molecular, 15054-1000 São José do Rio Preto, São Paulo, Brazil. Electronic address: carareto@ibilce.unesp.br.
authors.forename: Claudia M A
authors.initials: CM
authors.lastname: Carareto
authors.affiliation: UNESP-Univ. Estadual Paulista, Departamento de Biologia, Laboratório de Evolução Molecular, 15054-1000 São José do Rio Preto, São Paulo, Brazil.
authors.forename: Eric H
authors.initials: EH
authors.lastname: Hernandez
authors.affiliation: Université de Lyon, F-69000, Lyon, Université Lyon 1, CNRS, UMR5558, Laboratoire de Biométrie et Biologie Evolutive, F-69622, Villeurbanne, France; Institut Universitaire de France, France.
authors.forename: Cristina
authors.initials: C
authors.lastname: Vieira
mesh_terms: D024506:5' Flanking Region; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004251:DNA Transposable Elements; D004330:Drosophila / Q000235:genetics / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics; D005786:Gene Expression Regulation; D007305:Insecticide Resistance / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D014157:Transcription Factors; D003577:Cytochrome P-450 Enzyme System
keywords: CDS; Cyp; Cytochrome P450 monooxygenases; DINE-1; DNAREP1; Drosophila interspersed element 1; Gene expression regulation; LTR; MITE; SINE; TEs; TFBS; TIR; Transposable elements; coding sequence; cytochrome P450 monooxygenases; long terminal repeat; miniature inverted-repeat transposable element; nt; nucleotide; short interspersed nuclear element; terminal inverted repeat; transcription factor binding site; transposable elements