title: Establishment and genetic characterization of ANGM-CSS, a novel, immortal cell line derived from a human glioblastoma multiforme.
abstract: Glioblastoma multiforme (World Health Organization, grade IV astrocytoma) is the most common and most aggressive malignant primary brain tumor. We report a novel cell line, designated as ANGM-CSS, which was established from a 56-year-old male patient with a surgically removed glioblastoma multiforme. The ANGM-CSS cell line was established in vitro and characterized using histological and immunohistochemical staining, classical and molecular cytogenetic analyses, molecular studies and functional assays using a xenograft model in immunodeficient animals. ANGM-CSS was positive for CD133, nestin and vimentin proteins, whereas GFAP showed staining only in a fraction of the cells. Cytogenetic and molecular cytogenetic analysis revealed a near-tetraploid karyotype, with a modal chromosome number from 88 to 91, and additional cytogenetic abnormalities, such as the t(6;14)(p12;q11.2), t(8;10)(q24.2;q21.1) and t(5;9)(q34;p21) unbalanced translocations. Moreover, ANGM-CSS showed amplification of the MET and EGFR genes whose overexpression was observed at the mRNA level. Interestingly, ANGM-CSS is tumorigenic when implanted in immunodeficient mice, and the cells obtained from the xenografts showed the same morphology and karyotype in vitro as the original cell line. ANGM-CSS represents a biologically relevant cell line to be used to investigate the molecular pathology of glioblastoma multiforme, also to evaluate the efficacy of novel therapeutic drugs in vitro.
authors.affiliation: Medical Genetics Unit, IRCCS Casa Sollievo della Sofferenza Hospital, I-71013 San Giovanni Rotondo (FG), Italy.
authors.forename: Angelantonio
authors.initials: A
authors.lastname: Notarangelo
authors.affiliation: Laboratory of Oncology, IRCCS Casa Sollievo della Sofferenza Hospital, I-71013 San Giovanni Rotondo (FG), Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Trombetta
authors.affiliation: Department of Neurosurgery, IRCCS Casa Sollievo della Sofferenza Hospital, I-71013 San Giovanni Rotondo (FG), Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: D'Angelo
authors.affiliation: Laboratory of Oncology, IRCCS Casa Sollievo della Sofferenza Hospital, I-71013 San Giovanni Rotondo (FG), Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Parrella
authors.affiliation: Medical Genetics Unit, IRCCS Casa Sollievo della Sofferenza Hospital, I-71013 San Giovanni Rotondo (FG), Italy.
authors.forename: Orazio
authors.initials: O
authors.lastname: Palumbo
authors.affiliation: Department of Biology, University of Bari Aldo Moro, I-70125 Bari, Italy.
authors.forename: Clelia Tiziana
authors.initials: CT
authors.lastname: Storlazzi
authors.affiliation: Department of Biology, University of Bari Aldo Moro, I-70125 Bari, Italy.
authors.forename: Luciana
authors.initials: L
authors.lastname: Impera
authors.affiliation: Laboratory of Oncology, IRCCS Casa Sollievo della Sofferenza Hospital, I-71013 San Giovanni Rotondo (FG), Italy.
authors.forename: Lucia Anna
authors.initials: LA
authors.lastname: Muscarella
authors.affiliation: Laboratory of Oncology, IRCCS Casa Sollievo della Sofferenza Hospital, I-71013 San Giovanni Rotondo (FG), Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: La Torre
authors.affiliation: Biogem IRGS, I-83031 Ariano Irpino (AV), Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Affuso
authors.affiliation: Laboratory of Oncology, IRCCS Casa Sollievo della Sofferenza Hospital, I-71013 San Giovanni Rotondo (FG), Italy.
authors.forename: Vito Michele
authors.initials: VM
authors.lastname: Fazio
authors.affiliation: Medical Genetics Unit, IRCCS Casa Sollievo della Sofferenza Hospital, I-71013 San Giovanni Rotondo (FG), Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Carella
authors.affiliation: Medical Genetics Unit, IRCCS Casa Sollievo della Sofferenza Hospital, I-71013 San Giovanni Rotondo (FG), Italy.
authors.forename: Leopoldo
authors.initials: L
authors.lastname: Zelante
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D005909:Glioblastoma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D007621:Karyotyping; D051379:Mice