title: A high sensitivity MEA probe for measuring real time rat brain glucose flux.
abstract: The mammalian central nervous system (CNS) relies on a constant supply of external glucose for its undisturbed operation. This article presents an implantable Multi-Electrode Array (MEA) probe for brain glucose measurement. The MEA was implemented on Silicon-On-Insulator (SOI) wafer using Micro-Electro-Mechanical-Systems (MEMS) methods. There were 16 platinum recording sites on the probe and enzyme glucose oxidase (GOx) was immobilized on them. The glucose sensitivity of the MEA probe was as high as 489 µA mM(-1) cm(-2). 1,3-Phenylenediamine (mPD) was electropolymerized onto the Pt recording surfaces to prevent larger molecules such as ascorbic acid (AA), 3,4-dihydroxyphenylacetic acid (DOPAC), serotonin (5-HT), and dopamine (DA) from reaching the recording sites surface. The MEA probe was implanted in the anesthetized rat striatum and responded to glucose levels which were altered by intraperitoneal injection of glucose and insulin. After the in vivo experiment, the MEA probe still kept sensitivity to glucose, these suggested that the MEA probe was reliable for glucose monitoring in brain extracellular fluid (ECF).
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Song
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Nansen
authors.initials: N
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Tingjun
authors.initials: T
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100190, China. Electronic address: xxcai@mail.ie.ac.cn.
authors.forename: Xinxia
authors.initials: X
authors.lastname: Cai
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D003199:Computer Systems; D003217:Conductometry / Q000295:instrumentation*; D003342:Corpus Striatum / Q000378:metabolism*; D004567:Electrodes, Implanted; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis / Q000378:metabolism*; D005949:Glucose Oxidase / Q000737:chemistry*; D008297:Male; D064688:Metabolic Flux Analysis / Q000295:instrumentation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D005947:Glucose
keywords: Glucose biosensor; MEMS; Microelectrode; Rat brain; Real-time monitoring