title: Antioxidant Potential of Crude Extract and Different Fractions of Enhydra fluctuans Lour.
abstract: The antioxidant potential of crude methanol extract (CE) as well as chloroform (CF), ethyl acetate (EF) and n-butanol (NF) soluble fractions of Enhydra fluctuans Lour, which is widely used in indigenous system of medicine for different purposes, were studied. The antioxidant potential of extract/different fractions were evaluated using different in vitro antioxidant models. In addition, total amount of polyphenolics compounds, DPPH (1,1-diphenyl-2-picryl hydrazyl) radical, nitric oxide, superoxide anion, hydroxyl radical scavenging activities and reductive power of crude extracted its different fractions were determined. It was found that ethyl acetate fraction have maximum amount of polyphenolics compounds (179.7 ± 18.23 μg / mg pyrocatechol equivalent). This fraction was found more effective than crude extract and other fractions in all the above mentioned assays.
authors.affiliation: St. Peter's Institute of Pharmaceutical Sciences, Warangal, Andhra Pradesh -506001, India.
authors.forename: Santanu
authors.initials: S
authors.lastname: Sannigrahi
authors.affiliation: Department of Pharmaceutical Technology, Jadavpur University, Kolkata-700 032, West Bengal, India.
authors.forename: Upal
authors.initials: U
authors.lastname: Kanti Mazuder
authors.affiliation: Department of Pharmaceutical Chemistry, Seemanta Institute of Pharmaceutical Sciences, Jharpokharia, Mayurbhanj-757 086, Orissa, India.
authors.forename: Dilip
authors.initials: D
authors.lastname: Kumar Pal
authors.affiliation: St. Peter's Institute of Pharmaceutical Sciences, Warangal, Andhra Pradesh -506001, India.
authors.forename: Sambit
authors.initials: S
authors.lastname: Parida
authors.affiliation: St. Peter's Institute of Pharmaceutical Sciences, Warangal, Andhra Pradesh -506001, India.
authors.forename: Sourabh
authors.initials: S
authors.lastname: Jain
keywords: Antioxidant activity; Different fractions; Enhydra fluctuans; Flavonoids; Free radical scavenging activity