title: Effect of topiramate on interleukin 6 expression in the hippocampus of amygdala-kindled epileptic rats.
abstract: The objective of this study was to analyze the changes in expression and the possible functions of interleukin-6 (IL-6) in electrical kindling of the basolateral amygdala (BLA) in epileptic rats. Bipolar electrodes were implanted into the BLA of Sprague-Dawley rats, and the rats were then subjected to chronic electrical stimulation through the electrodes to induce kindling. The seizure characteristics and behavioral changes of the rats were observed, and electroencephalograms were recorded during and following kindling. The IL-6 mRNA expression in the hippocampi of the rats was analyzed using semi-quantitative reverse transcription-polymerase chain reaction, and control and topiramate (TPM)-treated groups were compared. The mean time-period required for kindling was 13.50±3.99 days, and the afterdischarge duration (ADD) measured between 21,450 and 119,720 msec. The expression of IL-6 mRNA was significantly upregulated in the kindled rats. TPM was able to depress the seizures and decrease the IL-6 level in the kindled rats. In conclusion, IL-6 mRNA was upregulated in the hippocampi of epileptic rats, and IL-6 may have participated in the process of kindling.
authors.affiliation: Department of Neurology, Jinhua Municipal Central Hospital, Jinhua, Zhejiang 321000, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Ye
authors.affiliation: Department of Neurology, Affiliated Children's Hospital of Soochow University, Suzhou, Jiangsu 215000, P.R. China.
authors.forename: Xu-Qin
authors.initials: XQ
authors.lastname: Chen
authors.affiliation: Department of Neurology, No.3 People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 201900, P.R. China.
authors.forename: Guan-Shui
authors.initials: GS
authors.lastname: Bao
authors.affiliation: Department of Child Neurology, Wuxi People's Hospital, Wuxi, Jiangsu 214001, P.R. China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Hua
authors.affiliation: Department of Neurology, Affiliated Children's Hospital of Soochow University, Suzhou, Jiangsu 215000, P.R. China.
authors.forename: Zhe-Dong
authors.initials: ZD
authors.lastname: Wang
authors.affiliation: Xi'an Jiaotong-Liverpool University, Suzhou, Jiangsu 215123, P.R. China.
authors.forename: Yi-Chuan
authors.initials: YC
authors.lastname: Bao
keywords: basolateral amygdale; epilepsy; interleukin-6; kindling; rat; topiramate