title: Acetaldehyde content and oxidative stress in the deleterious effects of alcohol drinking on rat uterine horn.
abstract: After alcohol exposure through a standard Lieber and De Carli diet for 28 days, a severe atrophy in the rat uteirne horn was observed, accompanied by significant alterations in its epithelial cells. Microsomal pathway of acetaldehyde production was slightly increased. Hydroxyl radicals were detected in the cytosolic fraction, and this was attributed to participation of xanthine oxidoreductase. They were also observed in the microsomal fraction in the presence of NADPH generating system. No generation of 1-hydroxyethyl was evidenced. The t-butylhydroperoxide-induced chemiluminescence analysis of uterine horn homogenates revealed a significant increase in the chemiluminiscence emission due to ethanol exposure. In the animals repeatedly exposed to alcohol, sulfhydryl content from uterine horn proteins was decreased, but no significant changes were observed in the protein carbonyl content from the same samples. Minor but significant decreasing changes were observed in the GSH content accompanied by a tendency to decrease in the GSH/GSSG ratio. A highly significant finding was the diminished activity content of glutathione peroxidase. Results suggest that acetaldehyde accumulation plus the oxidative stress may play an additional effect to the alcohol-promoted hormonal changes in the uterus reported by others after chronic exposure to alcohol.
authors.affiliation: Centro de Investigaciones Toxicológicas (CEITOX-UNIDEF, MINDEF-CONICET), Juan B de La Salle 4397, B1603ALO Villa Martelli, Argentina.
authors.forename: Lara Romina
authors.initials: LR
authors.lastname: Buthet
authors.affiliation: Centro de Investigaciones Toxicológicas (CEITOX-UNIDEF, MINDEF-CONICET), Juan B de La Salle 4397, B1603ALO Villa Martelli, Argentina ; Instituto de Investigación e Ingeniería Ambiental, Universidad Nacional de General San Martín, Avenue 25 de Mayo y Francia, 1650 San Martín, Argentina.
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Maciel
authors.affiliation: Centro de Investigaciones Toxicológicas (CEITOX-UNIDEF, MINDEF-CONICET), Juan B de La Salle 4397, B1603ALO Villa Martelli, Argentina ; Instituto de Investigación e Ingeniería Ambiental, Universidad Nacional de General San Martín, Avenue 25 de Mayo y Francia, 1650 San Martín, Argentina.
authors.forename: Leandro Néstor
authors.initials: LN
authors.lastname: Quintans
authors.affiliation: Centro de Investigaciones Toxicológicas (CEITOX-UNIDEF, MINDEF-CONICET), Juan B de La Salle 4397, B1603ALO Villa Martelli, Argentina.
authors.forename: Carmen
authors.initials: C
authors.lastname: Rodríguez de Castro
authors.affiliation: Centro de Investigaciones Toxicológicas (CEITOX-UNIDEF, MINDEF-CONICET), Juan B de La Salle 4397, B1603ALO Villa Martelli, Argentina ; Instituto de Investigación e Ingeniería Ambiental, Universidad Nacional de General San Martín, Avenue 25 de Mayo y Francia, 1650 San Martín, Argentina.
authors.forename: Martín Hernán
authors.initials: MH
authors.lastname: Costantini
authors.affiliation: Centro de Investigaciones Toxicológicas (CEITOX-UNIDEF, MINDEF-CONICET), Juan B de La Salle 4397, B1603ALO Villa Martelli, Argentina.
authors.forename: Silvia Laura
authors.initials: SL
authors.lastname: Fanelli
authors.affiliation: Centro de Investigaciones Toxicológicas (CEITOX-UNIDEF, MINDEF-CONICET), Juan B de La Salle 4397, B1603ALO Villa Martelli, Argentina ; Instituto de Investigación e Ingeniería Ambiental, Universidad Nacional de General San Martín, Avenue 25 de Mayo y Francia, 1650 San Martín, Argentina.
authors.forename: José Alberto
authors.initials: JA
authors.lastname: Castro
authors.affiliation: Centro de Investigaciones Toxicológicas (CEITOX-UNIDEF, MINDEF-CONICET), Juan B de La Salle 4397, B1603ALO Villa Martelli, Argentina ; Instituto de Investigación e Ingeniería Ambiental, Universidad Nacional de General San Martín, Avenue 25 de Mayo y Francia, 1650 San Martín, Argentina.
authors.forename: Gerardo Daniel
authors.initials: GD
authors.lastname: Castro