title: Composite poly-L-lactic acid/poly-(α,β)-DL-aspartic acid/collagen nanofibrous scaffolds for dermal tissue regeneration.
abstract: Tissue engineering scaffolds for skin tissue regeneration is an ever expounding area of research, as the products that meet the necessary requirements are far and elite. The nanofibrous poly-L-lactic acid/poly-(α,β)-DL-aspartic acid/Collagen (PLLA/PAA/Col I&III) scaffolds were fabricated by electrospinning and characterized by SEM, contact angle and FTIR analysis for skin tissue regeneration. The cell-scaffold interactions were analyzed by cell proliferation and their morphology observed in SEM. The results showed that the cell proliferation was significantly increased (p≤0.05) in PLLA/PAA/Col I&III scaffolds compared to PLLA and PLLA/PAA nanofibrous scaffolds. The abundance and accessibility of adipose derived stem cells (ADSCs) may prove to be novel cell therapeutics for dermal tissue regeneration. The differentiation of ADSCs was confirmed using collagen expression and their morphology by CMFDA dye extrusion technique. The current study focuses on the application of PLLA/PAA/Col I&III nanofibrous scaffolds for skin tissue engineering and their potential use as substrate for the culture and differentiation of ADSCs. The objective for inclusion of a novel cell binding moiety like PAA was to replace damaged extracellular matrix and to guide new cells directly into the wound bed with enhanced proliferation and overall organization. This combinatorial epitome of PLLA/PAA/Col I&III nanofibrous scaffold with stem cell therapy to induce the necessary paracrine signalling effect would favour faster regeneration of the damaged skin tissues.
authors.affiliation: Healthcare and Energy Materials Laboratory, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, 117576, Singapore; Department of Mechanical Engineering, National University of Singapore, 117576, Singapore.
authors.forename: Rajeswari
authors.initials: R
authors.lastname: Ravichandran
authors.affiliation: Healthcare and Energy Materials Laboratory, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, 117576, Singapore.
authors.forename: Jayarama Reddy
authors.initials: JR
authors.lastname: Venugopal
authors.affiliation: Healthcare and Energy Materials Laboratory, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, 117576, Singapore; Department of Mechanical Engineering, National University of Singapore, 117576, Singapore.
authors.forename: Subramanian
authors.initials: S
authors.lastname: Sundarrajan
authors.affiliation: Healthcare and Energy Materials Laboratory, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, 117576, Singapore.
authors.forename: Shayanti
authors.initials: S
authors.lastname: Mukherjee
authors.affiliation: Healthcare and Energy Materials Laboratory, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, 117576, Singapore; Department of Mechanical Engineering, National University of Singapore, 117576, Singapore.
authors.forename: Radhakrishnan
authors.initials: R
authors.lastname: Sridhar
authors.affiliation: Healthcare and Energy Materials Laboratory, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, 117576, Singapore; Department of Mechanical Engineering, National University of Singapore, 117576, Singapore.
authors.forename: Seeram
authors.initials: S
authors.lastname: Ramakrishna
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000737:chemistry* / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry* / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000187:drug effects; D019344:Lactic Acid / Q000737:chemistry* / Q000494:pharmacology*; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology*; D011817:Rabbits; D012038:Regeneration / Q000187:drug effects; D012867:Skin / Q000187:drug effects; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D011091:Polyesters; D011108:Polymers; D001224:Aspartic Acid; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen