title: Production and characterization of monoclonal antibodies against aflatoxin B1.
abstract: In this article, we embarked on production of mouse monoclonal antibodies against aflatoxin B1 which is the most commonly occurring fungal toxin in food and feed products. After immunization and fusion with myloma cells, two stable clones (A218 and B319) were selected. Isotyping showed that these monoclonal antibodies (mAbs) were IgG2b with kappa light chains. The affinity of A218 and B319 clons were 5×10(11) M(-1) and 6×10(9) M(-1), respectively. Competitive indirect ELISA results indicated these mAbs had complete (100%) cross-reaction with four major types of aflatoxins: B1, B2, G1, and G2. These mAbs could be used for immunoassay measurement of aflatoxins with high affinity and low detection limits.
authors.affiliation: a Department of Clinical Biochemistry, School of Medicine , Mashhad University of Medical Sciences , Mashhad , Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Soukhtanloo
authors.forename: Elham
authors.initials: E
authors.lastname: Talebian
authors.forename: Mehdi
authors.initials: M
authors.lastname: Golchin
authors.forename: Mojgan
authors.initials: M
authors.lastname: Mohammadi
authors.forename: Bagher
authors.initials: B
authors.lastname: Amirheidari
mesh_terms: D016604:Aflatoxin B1 / Q000008:administration & dosage / Q000097:blood / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D002417:Cattle; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D016604:Aflatoxin B1
keywords: ELISA; aflatoxin B1; monoclonal antibody