title: Triggering cell death by nanographene oxide mediated hyperthermia.
abstract: Graphene oxide (GO) has been proposed as an hyperthermia agent for anticancer therapies due to its near-infrared (NIR) optical absorption ability which, with its small two-dimensional size, could have a unique performance when compared to that of any other nanoparticle. Nevertheless, attention should be given to the hyperthermia route and the kind of GO-cell interactions induced in the process. The hyperthermia laser irradiation parameters, such as exposure time and laser power, were investigated to control the temperature rise and consequent damage in the GOs containing cell culture medium. The type of cell damage produced was evaluated as a function of these parameters. The results showed that cell culture temperature (after irradiating cells with internalized GO) increases preferentially with laser power rather than with exposure time. Moreover, when laser power is increased, necrosis is the preferential cell death leading to an increase of cytokine release to the medium.
authors.affiliation: Department of Inorganic and Bioinorganic Chemistry, Faculty of Pharmacy, UCM, Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, E-28040-Madrid, Spain. Networking Research Center on Bioengineering, Biomaterials and Nanomedicine, CIBER-BBN, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Vila
authors.forename: M C
authors.initials: MC
authors.lastname: Matesanz
authors.forename: G
authors.initials: G
authors.lastname: Gonçalves
authors.forename: M J
authors.initials: MJ
authors.lastname: Feito
authors.forename: J
authors.initials: J
authors.lastname: Linares
authors.forename: P A A P
authors.initials: PA
authors.lastname: Marques
authors.forename: M T
authors.initials: MT
authors.lastname: Portolés
authors.forename: M
authors.initials: M
authors.lastname: Vallet-Regi
mesh_terms: D016923:Cell Death / Q000187:drug effects* / Q000276:immunology; D045744:Cell Line, Tumor; D016207:Cytokines / Q000378:metabolism*; D055032:Electron Microscope Tomography; D006108:Graphite / Q000494:pharmacology*; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D007834:Lasers; D018613:Microscopy, Confocal; D053758:Nanoparticles; D010006:Osteoblasts; D010087:Oxides
chemical_list: D016207:Cytokines; D010087:Oxides; D006108:Graphite