title: Impact of bioaugmentation on biochemical methane potential for wheat straw with addition of Clostridium cellulolyticum.
abstract: Hydrolysis is usually the rate-limited step for methane production from lignocellulosic substrate. Two bioaugmentation strategies, using the cellulolytic anaerobic bacteria Clostridium cellulolyticum, were adopted to enhance the hydrolysis of wheat straw with the purpose of improving the biochemical methane potential (BMP). Namely, the 24-h-incubated seed (C24S) with cellobiose as carbon source and the 60-h-incubated seed (WS60S) with wheat straw as carbon source were respectively used as the bioaugmentation agents. As a result, the BMPs were respectively 342.5 and 326.3 ml g(-1) VS of wheat straw, with an increase of 13.0% and 7.6% comparing to the no-bioaugmentation BMP of 303.3 ml g(-1) VS. The result indicates that the anaerobic digestion efficiency can be improved by bioaugmentation, which therefore may be a promising method for improving methane production from lignocellulosic substrate.
authors.affiliation: Department of Biotechnology, Lund University, Box 124, SE-22100 Lund, Sweden; State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Biotechnology, Lund University, Box 124, SE-22100 Lund, Sweden.
authors.forename: Rosa Aragão
authors.initials: RA
authors.lastname: Börner
authors.affiliation: Department of Biotechnology, Lund University, Box 124, SE-22100 Lund, Sweden.
authors.forename: Ivo Achu
authors.initials: IA
authors.lastname: Nges
authors.affiliation: Department of Biotechnology, Lund University, Box 124, SE-22100 Lund, Sweden; Bioprocess Control AB, Scheelevägen 22, SE-22363 Lund, Sweden. Electronic address: jing.liu@biotek.lu.se.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
mesh_terms: D001673:Biodegradation, Environmental; D002480:Cellulase / Q000378:metabolism; D046973:Ruminiclostridium cellulolyticum / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D008697:Methane / Q000378:metabolism*; D013997:Time Factors; D014908:Triticum / Q000378:metabolism*; D014866:Waste Products
chemical_list: D014866:Waste Products; D002480:Cellulase; D008697:Methane
keywords: Anaerobic digestion; Bioaugmentation; Biogas; Cellulase; Straw