title: Effects of habitat light conditions on the excitation quenching pathways in desiccating Haberlea rhodopensis leaves: an Intelligent FluoroSensor study.
abstract: Resurrection plants can survive dehydration to air-dry state, thus they are excellent models of understanding drought and dehydration tolerance mechanisms. Haberlea rhodopensis, a chlorophyll-retaining resurrection plant, can survive desiccation to relative water content below 10%. Leaves, detached from plants of sun and shade habitats, were moderately (∼50%) dehydrated in darkness. During desiccation, chlorophyll a fluorescence was detected by the recently innovated wireless Intelligent FluoroSensor (IFS) chlorophyll fluorometer, working with three different detectors: a pulse-amplitude-modulated (PAM) broadband channel and two channels to measure non-modulated red and far-red fluorescence. No change in area-based chlorophyll content of leaves was observed. The maximal quantum efficiency of photosystem II decreased gradually in both shade and sun leaves. Shade leaves could not increase antennae-based quenching, thus inactivated photosystem II took part in quenching of excess irradiation. Sun leaves seemed to be pre-adapted to quench excess light as they established an intensive increase in antennae-based non-photochemical quenching parallel to desiccation. The higher far-red to red antennae-based quenching may sign light-harvesting complex reorganization. Thus, compared to PAM, IFS chlorophyll fluorometer has additional benefits including (i) parallel estimation of changes in the Chl content and (ii) prediction of underlying processes of excitation energy quenching.
authors.affiliation: Department of Plant Physiology and Molecular Plant Biology, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117 Budapest, Hungary. Electronic address: sadambio@elte.hu.
authors.forename: Ádám
authors.initials: Á
authors.lastname: Solti
authors.affiliation: Department of Atomic Physics, Budapest University of Technology and Economics, Budafoki út 8, H-1111 Budapest, Hungary. Electronic address: lenk@eik.bme.hu.
authors.forename: Sándor
authors.initials: S
authors.lastname: Lenk
authors.affiliation: Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Akad. Georgi Bonchev Str., Bl. 21, BG-1113 Sofia, Bulgaria. Electronic address: gkm_rw@abv.bg.
authors.forename: Gergana
authors.initials: G
authors.lastname: Mihailova
authors.affiliation: Department of Atomic Physics, Budapest University of Technology and Economics, Budafoki út 8, H-1111 Budapest, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Mayer
authors.affiliation: Department of Atomic Physics, Budapest University of Technology and Economics, Budafoki út 8, H-1111 Budapest, Hungary. Electronic address: barocsi@eik.bme.hu.
authors.forename: Attila
authors.initials: A
authors.lastname: Barócsi
authors.affiliation: Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Akad. Georgi Bonchev Str., Bl. 21, BG-1113 Sofia, Bulgaria. Electronic address: katya@bio21.bas.bg.
authors.forename: Katya
authors.initials: K
authors.lastname: Georgieva
mesh_terms: D002734:Chlorophyll / Q000378:metabolism*; D000077194:Chlorophyll A; D003681:Dehydration / Q000378:metabolism*; D017753:Ecosystem; D005453:Fluorescence; D005470:Fluorometry / Q000379:methods*; D008027:Light; D018515:Plant Leaves / Q000378:metabolism*; D064028:Tracheophyta
chemical_list: D002734:Chlorophyll; D000077194:Chlorophyll A
keywords: Excitation energy allocation; Far-red fluorescence; Haberlea rhodopensis; Intelligent FluoroSensor; Resurrection plant