title: Butcherbird polyomavirus isolated from a grey butcherbird (Cracticus torquatus) in Queensland, Australia.
abstract: A novel avian polyomavirus was detected in peri-ocular skin lesions collected from a grey butcherbird (Cracticus torquatus), using a combination of multiply primed rolling circle amplification, nested PCR and long range PCR. The sequence of Butcherbird polyomavirus was determined by combining next generation sequencing and primer walking techniques. The circular double-stranded DNA genome of Butcherbird polyomavirus consisted of 5084 bp, and encoded six open reading frames (ORF-X, VP2, VP3, VP1, small T-antigen and large T-antigen). Phylogenetic analysis placed it amongst other members of the genus Avipolyomavirus, most closely related to Crow polyomavirus. Next generation sequencing enabled the detection of DNA fragments similar to, but distinct from, Canarypox virus within the same lesion from which Butcherbird polyomavirus was amplified, thus confirming an avipolyomavirus-avipoxvirus co-infection in the peri-ocular skin lesions of this grey butcherbird.
authors.affiliation: School of Veterinary and Life Sciences, Murdoch University, South Street, Murdoch 6150, WA, Australia. Electronic address: m.bennett@murdoch.edu.au.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Bennett
authors.affiliation: Australia Zoo Wildlife Hospital, Beerwah 4519, QLD, Australia.
authors.forename: Amber
authors.initials: A
authors.lastname: Gillett
mesh_terms: D000818:Animals; D000835:Animals, Wild; D018150:Avipoxvirus / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D060085:Coinfection / Q000821:virology; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D046109:Passeriformes / Q000235:genetics / Q000821:virology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011120:Polyomavirus / Q000235:genetics / Q000302:isolation & purification*; D027601:Polyomavirus Infections / Q000662:veterinary*; D011213:Poxviridae Infections / Q000662:veterinary*; D011793:Queensland; D014412:Tumor Virus Infections / Q000662:veterinary*
keywords: Butcherbird; Co-infection; Cracticus torquatus; Polyomavirus; Poxvirus