title: Breaking pseudo-symmetry in multiantennary complex N-glycans using lanthanide-binding tags and NMR pseudo-contact shifts.
abstract: Controlling NMR shifts by lanthanides tagged to a "symmetrical" N-glycan reveals individual resonances for the residues of the otherwise identical A and B arms. This method provides a global perspective of conformational features and interactions in solution.
authors.affiliation: Departamento de Química Orgánica I, Fac. C. C. Químicas, Universidad Complutense de Madrid, 28040 Madrid (Spain).
authors.forename: Angeles
authors.initials: A
authors.lastname: Canales
authors.forename: Alvaro
authors.initials: A
authors.lastname: Mallagaray
authors.forename: Javier
authors.initials: J
authors.lastname: Pérez-Castells
authors.forename: Irene
authors.initials: I
authors.lastname: Boos
authors.forename: Carlo
authors.initials: C
authors.lastname: Unverzagt
authors.forename: Sadine
authors.initials: S
authors.lastname: André
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Gabius
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Cañada
authors.forename: Jesús
authors.initials: J
authors.lastname: Jiménez-Barbero
mesh_terms: D028581:Lanthanoid Series Elements / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008958:Models, Molecular; D011134:Polysaccharides
chemical_list: D028581:Lanthanoid Series Elements; D011134:Polysaccharides
keywords: N-glycans; NMR spectroscopy; lanthanides; molecular recognition; pseudo-contact shifts