title: Spatiotemporal changes in NFATc4 expression of retinal ganglion cells after light-induced damage.
abstract: Nuclear factor of activated T cells, cytoplasmic 4 (NFATc4) is one of the four members of the NFAT family, which were described first as essential components of T cells activation and lately as important regulators for the initiation and coordination of the immune response, including B cells and natural killer cells. Accumulating evidence has demonstrated that NFATc4 exerted a pro-apoptotic effect in the pathogenesis of various experimental central nervous system diseases by upregulating Fas ligand (FasL) levels. However, the function of NFATc4 in the retina is still with limited acquaintance. To investigate whether NFATc4 is involved in retinal neuron apoptosis, we performed a light-induced retinal damage model in adult rats. A significant upregulation of NFATc4 was detected in the retina after light-induced damage by using Western blotting and reverse transcriptase PCR (RT-PCR). Besides this, NFATc4 was observed to be localized mainly in the retinal ganglion cells (RGCs). In addition, the expression patterns of active caspase-3, active caspase-8, and FasL were parallel with that of NFATc4. We also found the co-localization of NFATc4 with active caspase-3 and FasL in RGCs after light exposure. Collectively, we hypothesized that NFATc4 might participate in RGCs apoptosis by upregulating FasL levels.
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong Province, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Xu
authors.forename: Lu
authors.initials: L
authors.lastname: Yang
authors.forename: Shanshan
authors.initials: S
authors.lastname: Yu
authors.forename: Qinmeng
authors.initials: Q
authors.lastname: Shu
authors.forename: Cheng
authors.initials: C
authors.lastname: Yang
authors.forename: Jiawei
authors.initials: J
authors.lastname: Wang
authors.forename: Fan
authors.initials: F
authors.lastname: Xu
authors.forename: Aimin
authors.initials: A
authors.lastname: Sang
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Liang
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053181:Caspase 8 / Q000235:genetics / Q000378:metabolism; D053222:Fas Ligand Protein / Q000235:genetics / Q000378:metabolism; D008027:Light / Q000009:adverse effects*; D050778:NFATC Transcription Factors / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D051381:Rats; D017207:Rats, Sprague-Dawley; D012165:Retinal Ganglion Cells / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects
chemical_list: D053222:Fas Ligand Protein; D050778:NFATC Transcription Factors; D053148:Caspase 3; D053181:Caspase 8