title: [Transforming growth factor β1 enhances the invasiveness of human glioma cell line via ERK/MAPK pathway].
abstract: OBJECTIVE
  To investigate the effect of transforming growth factor β1 (TGF-β1) on the invasiveness of human glioma SF767 cell line in vitro and explore the possible molecular mechanism.
  
  
  METHODS
  Human glioma SF767 cell line treated with TGF-β1 for 12, 24, or 48 h were examined for the expressions of epithelial-mesenchymal transition- and ERK/MAPK pathway-associated proteins using Western blotting. MTT assay and Transwell assay were employed to assess the changes in the cell proliferation and invasiveness after TGF-β1 treatment, respectively.
  
  
  RESULTS
  Western blotting showed that TGF-β1 treatment up-regulated the expressions of vimentin, MMP-2, P-ERK, and Zeb-1 and down-regulated E-cadherin expression in SF767 cells. TGF-β1 treatment of the cells resulted in significantly shortened doubling time of cells and obviously increased cell invasiveness.
  
  
  CONCLUSIONS
  TGF-β1 induces epithelial-mesenchymal transition of SF767 cell line to increase its invasiveness possibly via ERK/MAPK pathway.
authors.affiliation: Department of Neurology, First Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Xi'an 710061, China. E-mail: chinaylh2003@163.com.
authors.forename: Lihua
authors.initials: L
authors.lastname: Liu
authors.forename: Qin
authors.initials: Q
authors.lastname: Dai
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Min
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
mesh_terms: D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition; D005910:Glioma / Q000473:pathology*; D006801:Humans; D020935:MAP Kinase Signaling System; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D014746:Vimentin / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; D014746:Vimentin; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2