title: Ultralow frequency Stokes and anti-Stokes Raman spectroscopy of single living cells and microparticles using a hot rubidium vapor filter.
abstract: We report on ultralow frequency Stokes and anti-Stokes Raman spectroscopy of single living cells and microsized particles in an aqueous medium with a frequency shift down to 10 cm(-1) by the combination of a hot rubidium (Rb) vapor filter, a confocal pinhole, and optical trapping. A single frequency-stabilized diode laser beam at 780.2 nm is used to optically trap and excite a single living cell or microparticle, and the Rayleigh scattering light from the particle is effectively blocked with a Rb vapor cell and a confocal pinhole. Ultralow frequency Raman spectra of the trapped cells or microparticles in both Stokes and anti-Stokes regions are then measured with a single-stage CCD spectrograph.
authors.forename: Jinda
authors.initials: J
authors.lastname: Lin
authors.forename: Yong-qing
authors.initials: YQ
authors.lastname: Li
mesh_terms: D002470:Cell Survival; D004912:Erythrocytes / Q000166:cytology; D006358:Hot Temperature; D008863:Microspheres; D052898:Optical Tweezers; D012413:Rubidium / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014835:Volatilization
chemical_list: D012413:Rubidium