title: Immunoproteomic to identify antigens in the intestinal mucosa of Crohn's disease patients.
abstract: Incidences of Crohn disease (CD) have increased significantly in the last decade. Immunoproteomics are a promising method to identify biomarkers of different diseases. In the present study, we used immunoproteomics to study proteins of intestinal mucosal lesions and neighboring normal intestinal mucosa of 8 CD patients. Reactive proteins were validated by Western blotting. Approximately 50 protein spots localized in the 4 to 7 pI range were detected on two-dimensional electrophoresis gels, and 6 differentially expressed protein spots between 10 and 100 kDa were identified. Reactive proteins were identified as prohibitin, calreticulin, apolipoprotein A-I, intelectin-1, protein disulfide isomerase, and glutathione s-transferase Pi. Western blotting was conducted on the intestinal mucosa of another 4 CD patients to validate the reactive proteins. We found that intestinal mucosal lesions had high levels of prohibitin expression. Glutathione s-transferase expression was detected in 100% of the intestinal mucosa examined. Thus, we report 6 autoantigens of CD, including 3 new and 3 previously reported autoantigens. Intelectin-1, protein disulfide isomerase, and glutathione-s-transferases may be used as biomarkers for CD pathogenesis.
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Anhui Medical University, Anhui, Hefei, P.R. China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Critical Care, First Affiliated Hospital of Anhui Medical University, Anhui, Hefei, P.R. China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Liu
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, P.R. China.
authors.forename: Guosheng
authors.initials: G
authors.lastname: Gu
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, P.R. China.
authors.forename: Gefei
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Anhui Medical University, Anhui, Hefei, P.R. China.
authors.forename: Wenyong
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Anhui Medical University, Anhui, Hefei, P.R. China.
authors.forename: Changle
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, P.R. China.
authors.forename: Jianan
authors.initials: J
authors.lastname: Ren
mesh_terms: D001324:Autoantigens / Q000276:immunology / Q000378:metabolism*; D003424:Crohn Disease / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism*; D040901:Proteomics / Q000379:methods
chemical_list: D001324:Autoantigens