title: [Palmitate induces apoptosis and endoplasmic reticulum stress in human umbilical cord-derived mesenchymal stem cells].
abstract: The saturated free fatty acid (FFA), palmitate, could induce apoptosis in various cell types, but little is known about its effects on human umbilical cord-derived mesenchymal stem cells (hUC-MSCs). Here, we investigated whether palmitate induced apoptosis and endoplasmic reticulum (ER) stress in hUC-MSCs. hUC-MSCs were stained by labeled antibodies and identified by flow cytometry. After administration with palmitate, apoptotic cell was assessed by flow cytometry using the Annexin V-FITC/7-AAD apoptosis detection kit. Relative spliced XBP1 levels were analyzed using semi-quantitative RT-PCR. The mRNA of BiP, GRP94, ATF4 and CHOP were analyzed by real-time PCR. Relative BiP and CHOP protein were analyzed using Western blot analysis. The results showed that hUC-MSCs were homogeneously positive for MSC markers; palmitate increased apoptosis of hUC-MSCs and activated XBP1 splicing, BiP, GRP94, ATF4 and CHOP transcription. These findings suggest that palmitate induces apoptosis and ER stress in hUC-MSCs.
authors.affiliation: Fujian Provincial Key Laboratory of Transplant Biology, Fuzhou General Hospital, Fuzhou 350025, China. hlhtiger@163.com, tanjm156@gmail.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
authors.forename: Hui-Yue
authors.initials: HY
authors.lastname: Dong
authors.forename: Ling-Jing
authors.initials: LJ
authors.lastname: Lin
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Wang
authors.forename: Liang-Hu
authors.initials: LH
authors.lastname: Huang
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Tan
mesh_terms: D051701:Activating Transcription Factor 4 / Q000378:metabolism; D017209:Apoptosis; D004268:DNA-Binding Proteins / Q000378:metabolism; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress; D006360:Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D010168:Palmitates / Q000494:pharmacology*; D000071701:Regulatory Factor X Transcription Factors; D051742:Transcription Factor CHOP / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology; D000071717:X-Box Binding Protein 1
chemical_list: C497109:ATF4 protein, human; C497249:DDIT3 protein, human; D004268:DNA-Binding Proteins; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; D008562:Membrane Glycoproteins; D010168:Palmitates; D000071701:Regulatory Factor X Transcription Factors; D014157:Transcription Factors; D000071717:X-Box Binding Protein 1; C000605543:XBP1 protein, human; C053387:endoplasmin; D051701:Activating Transcription Factor 4; D051742:Transcription Factor CHOP