title: Oxidative stress parameters in fish after subchronic exposure to acetylsalicylic acid.
abstract: OBJECTIVE
  The aim of this study was to investigate the effects of subchronic exposure of juvenile development stages of zebrafish (Danio rerio) to acetylsalicylic acid using selected oxidative stress biomarkers.
  
  
  METHODS
  Toxicity test with acetylsalicylic acid was performed according to the OECD Guideline No. 215, fish D. rerio aged 30 days were used. The tested concentrations were 0.004, 0.4, 40, 120 and 250 mg.L-1, duration of the test was 28 days. Products of lipid peroxidation and antioxidant enzymes were determined as the markers of oxidative stress.
  
  
  RESULTS
  Significantly increased glutathione S-transferase activity was found in fish exposed to acetylsalicylic acid concentrations 40, 120 and 250 mg.L-1. The highest values of glutathione reductase activity were found in the groups exposed to acetylsalicylic acid concentrations 0.4, 40 and 120 mg.L-1. In the group exposed to acetylsalicylic acid concentrations 40 mg.L-1, catalase activity was significantly higher compared to the control group. Significantly higher glutathione peroxidase activity was found in the groups exposed to acetylsalicylic acid concentrations 0.004 and 120 mg.L-1. The concentrations of TBARS were lower in fish exposed to acetylsalicylic acid at all tested concentrations compared to control.
  
  
  CONCLUSIONS
  The subchronic exposure of zebrafish to acetylsalicylic acid causes an increase in activity of antioxidant and biotransformation enzymes and a decrease in lipid peroxidation.
authors.affiliation: Department of Veterinary Public Health and Animal Welfare, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic. H11473@vfu.cz.
authors.forename: Dana
authors.initials: D
authors.lastname: Zivna
authors.affiliation: Department of Veterinary Public Health and Animal Welfare, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Plhalova
authors.affiliation: Department of Veterinary Public Health and Animal Welfare, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Praskova
authors.affiliation: Department of Veterinary Public Health and Animal Welfare, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
authors.forename: Stanislava
authors.initials: S
authors.lastname: Stepanova
authors.affiliation: Department of Veterinary Public Health and Animal Welfare, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Siroka
authors.affiliation: Department of Veterinary Public Health and Animal Welfare, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
authors.forename: Marie
authors.initials: M
authors.lastname: Sevcikova
authors.affiliation: Department of Veterinary Public Health and Animal Welfare, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Blahova
authors.affiliation: Department of Veterinary Public Health and Animal Welfare, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
authors.forename: Marta
authors.initials: M
authors.lastname: Bartoskova
authors.affiliation: Department of Veterinary Public Health and Animal Welfare, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Marsalek
authors.affiliation: Department of Pathological Morphology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
authors.forename: Misa
authors.initials: M
authors.lastname: Skoric
authors.affiliation: Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Svobodova
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001241:Aspirin / Q000633:toxicity*; D001522:Behavior, Animal / Q000187:drug effects; D004781:Environmental Exposure; D005399:Fishes / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D059550:Toxicity Tests, Subchronic; D014874:Water Pollutants, Chemical / Q000633:toxicity; D015027:Zebrafish
chemical_list: D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D014874:Water Pollutants, Chemical; D005982:Glutathione Transferase; D001241:Aspirin