title: Development and validation of an LC-MS/MS method for simultaneous quantitative analysis of free and conjugated bisphenol A in human urine.
abstract: Bisphenol A (BPA) is an environmental endocrine-disrupting chemicals that is widely used in common consumer products. There is an increasing concern regarding human exposure to BPA owing to the potential adverse effects associated with its estrogenic activity. For assessing environmental exposure to BPA, it is essential to have a sensitive, accurate and selective analytical method, especially one that can detect low BPA levels in complex sample matrices. In this study, we developed and validated an accurate, sensitive, and robust liquid chromatography-tandem mass spectrometry method for simultaneous quantification of free BPA and BPA β-d-glucuronide (BPA-gluc) concentrations in human urine with only a single injection. Calibration curves were linear over a concentration range of 1-100 ng/mL for BPA and 10-1000 ng/mL for BPA-gluc. The levels of the analytes were determined quantitatively with HPLC/ESI-MS/MS by using negative electrospray ionization in the select ion monitoring mode and a pentaflouraphenyl propyl column. The validated method was applied to the analysis of spot urine specimens collected from randomly selected healthy human subjects.
authors.affiliation: Mersin University, Faculty of Pharmacy, Department of Toxicology, 33169, Mersin, Turkey.
authors.forename: Dilek
authors.initials: D
authors.lastname: Battal
authors.forename: Ismet
authors.initials: I
authors.lastname: Cok
authors.forename: Irfan
authors.initials: I
authors.lastname: Unlusayin
authors.forename: Bahar
authors.initials: B
authors.lastname: Tunctan
mesh_terms: D000328:Adult; D001559:Benzhydryl Compounds / Q000737:chemistry / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D052244:Endocrine Disruptors / Q000737:chemistry / Q000652:urine*; D005260:Female; D006801:Humans; D008297:Male; D010636:Phenols / Q000737:chemistry / Q000652:urine*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: bisphenol A; endocrine disruptors; quantification; urine