title: [Fast screening of the artificial dyes in wine by liquid chromatography/hybrid linear ion trap-orbitrap mass spectrometry].
abstract: A fast screening method was established for the simultaneous determination of 15 water-soluble artificial dyes in wine by liquid chromatography/hybrid linear ion trap orbitrap mass spectrometry (LC/LTQ-orbitrap MS) based on a self-established mass spectral database. The confirmations of these target dyes were processed by the accurate mass numbers, and the MS(2) spectra marching to the self-established mass spectral database. The dyes in wine were purified by an anion-exchange column and separated by a BEH Phenyl column, then identified and quantified by LTQ-orbitrap MS. The results showed that the method detection limits of the target compounds were ranged from 0.00040 to 0.18 mg/L, and the average recoveries of the 15 compounds spiked at three concentrations were in the range of 43.1% - 127% with the relative standard deviations less than 10%. The spiked sample had the matching scores higher than 98% to the second order mass spectra of the standard compounds in the database. The method is also suitable for screening the 15 dyes in wine without reference standards.
authors.affiliation: Shenzhen Academy of Metrology and Quality Inspection, Shenzhen 518000, China.
authors.forename: Yongle
authors.initials: Y
authors.lastname: Li
authors.affiliation: Shenzhen Academy of Metrology and Quality Inspection, Shenzhen 518000, China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Shenzhen Academy of Metrology and Quality Inspection, Shenzhen 518000, China.
authors.forename: Cen
authors.initials: C
authors.lastname: Xiong
authors.affiliation: Shenzhen Academy of Metrology and Quality Inspection, Shenzhen 518000, China.
authors.forename: Yongting
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Shenzhen Academy of Metrology and Quality Inspection, Shenzhen 518000, China.
authors.forename: Sujuan
authors.initials: S
authors.lastname: Chen
mesh_terms: D002853:Chromatography, Liquid; D004396:Coloring Agents / Q000032:analysis*; D013058:Mass Spectrometry; D014920:Wine / Q000032:analysis*
chemical_list: D004396:Coloring Agents