title: The effects of luteolin on the intestinal ischemia/reperfusion injury in mice.
abstract: The purpose of this study is to investigate the potential protective effect of the flavonoid Luteolin on ischemia-reperfusion (IR) injury in mouse intestine, which has not previously been studied. Twenty-four female C57BL/6 mice were randomly assigned to four groups, each consisting of 6 mice: a sham group (laparotomy, but no IR injury), a sham + Luteolin group (no IR, and Luteolin was administered intraperitoneally 30 min after laparotomy), IR group (30 min occlusion of the superior mesenteric artery (SMA) then 2 hr' reperfusion), IR + Luteolin (30 min occlusion of the SMA then 2 hr' reperfusion; Luteolin was administered intraperitoneally before reperfusion). Intestine tissues were harvested from the mice for histopathological and biochemical analysis. Total oxidant status (TOS) and total antioxidant capacity (TAC) of the intestinal tissues were measured using Erel's method. Oxidative stress index (OSI) was calculated using the TOS/TAC ratio. Intestinal histological changes were significantly decreased in the IR + Luteolin group compared with the IR group (p = .037). TOS tissue levels were also significantly decreased in the IR + Luteolin group compared with the IR group (p = .005). TAC levels did not increase significantly in the IR treatment group and were not affected by Luteolin treatment (p > .05). The results of this study show that Luteolin administration provides considerable protection against IR injury in the mouse intestine.
authors.affiliation: 1Department of General Surgery, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Barış Rafet
authors.initials: BR
authors.lastname: Karakaş
authors.forename: Fatih
authors.initials: F
authors.lastname: Davran
authors.forename: Gülsüm Özlem
authors.initials: GÖ
authors.lastname: Elpek
authors.forename: S Halide
authors.initials: SH
authors.lastname: Akbaş
authors.forename: Kemal Hakan
authors.initials: KH
authors.lastname: Gülkesen
authors.forename: Nurullah
authors.initials: N
authors.lastname: Bülbüller
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D005260:Female; D007422:Intestines / Q000098:blood supply / Q000187:drug effects* / Q000293:injuries*; D047311:Luteolin / Q000494:pharmacology*; D017538:Mesenteric Artery, Superior / Q000293:injuries; D051379:Mice; D008810:Mice, Inbred C57BL; D016877:Oxidants / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*
chemical_list: D000975:Antioxidants; D016877:Oxidants; D047311:Luteolin
keywords: Luteolin; acute mesenteric ischemia; flavonoid; intestine; ischemia-reperfusion injury; mice