title: Ethanol production from glucose and xylose obtained from steam exploded water-extracted olive tree pruning using phosphoric acid as catalyst.
abstract: In this work, the effect of phosphoric acid (1% w/w) in steam explosion pretreatment of water extracted olive tree pruning at 175°C and 195°C was evaluated. The objective is to produce ethanol from all sugars (mainly glucose and xylose) contained in the pretreated material. The water insoluble fraction obtained after pretreatment was used as substrate in a simultaneous saccharification and fermentation (SSF) process by a commercial strain of Saccharomyces cerevisiae. The liquid fraction, containing mainly xylose, was detoxified by alkali and ion-exchange resin and then fermented by the xylose fermenting yeast Scheffersomyces stipitis. Ethanol yields reached in a SSF process were close to 80% when using 15% (w/w) substrate consistency and about 70% of theoretical when using prehydrolysates detoxified by ion-exchange resins. Considering sugars recovery and ethanol yields about 160g of ethanol from kg of water extracted olive tree pruning could be obtained.
authors.affiliation: Biofuels Unit, DER-CIEMAT, Avda. Complutense 40, 28040 Madrid, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Negro
authors.affiliation: Biofuels Unit, DER-CIEMAT, Avda. Complutense 40, 28040 Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Alvarez
authors.affiliation: Biofuels Unit, DER-CIEMAT, Avda. Complutense 40, 28040 Madrid, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Ballesteros
authors.affiliation: Department of Chemical, Environmental and Materials Engineering, University of Jaen, Campus Las Lagunillas, 23071 Jaen, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Romero
authors.affiliation: Biofuels Unit, DER-CIEMAT, Avda. Complutense 40, 28040 Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Ballesteros
authors.affiliation: Department of Chemical, Environmental and Materials Engineering, University of Jaen, Campus Las Lagunillas, 23071 Jaen, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Castro
authors.affiliation: Biofuels Unit, DER-CIEMAT, Avda. Complutense 40, 28040 Madrid, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Manzanares
authors.affiliation: Department of Chemical, Environmental and Materials Engineering, University of Jaen, Campus Las Lagunillas, 23071 Jaen, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Moya
authors.affiliation: Biofuels Unit, DER-CIEMAT, Avda. Complutense 40, 28040 Madrid, Spain. Electronic address: josemiguel.oliva@ciemat.es.
authors.forename: J M
authors.initials: JM
authors.lastname: Oliva
mesh_terms: D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002384:Catalysis / Q000187:drug effects; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D006868:Hydrolysis / Q000187:drug effects; D008049:Lipase / Q000378:metabolism; D050296:Microbial Viability / Q000187:drug effects; D031658:Olea / Q000737:chemistry*; D010756:Phosphoric Acids / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism; D012995:Solubility; D013227:Steam; D013997:Time Factors; D014197:Trees / Q000737:chemistry; D014866:Waste Products; D014994:Xylose / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D010756:Phosphoric Acids; D013227:Steam; D014866:Waste Products; D000431:Ethanol; D014994:Xylose; C030242:phosphoric acid; D008049:Lipase; D001617:beta-Glucosidase; D005947:Glucose
keywords: Detoxification; Olive tree pruning; Phosphoric acid; Saccharomyces cerevisiae; Scheffersomyces stipitis