title: Distinct expression profiles of LncRNAs between brown adipose tissue and skeletal muscle.
abstract: Both brown adipose tissue and skeletalmuscle have abundant mitochondria and energy consumption capacity. They are similar in origin and gain different potential of energy metabolism after differentiation and maturation. The mechanism that cause the difference is not yet fully understood. Long non-coding RNAs (lncRNAs) which comprise the bulk of the human non-coding transcriptome have been proved to play key roles in various biological processes. Whether they will have a function on the differentiation and energy metabolism between BAT and skeletalmuscle is still unknown. To identify the cellular long noncoding RNAs (lncRNAs) involved in the progress, we used the next generation transcriptome sequencing and microarray techniques, and investigated 704 up-regulated and 896 down-regulated lncRNAs (fold-change >3.0) in BAT by comparing the expression profile. Furthermore, we reported AK003288 associated with junctophilin 2 (Jph2) gene which may affect energy metabolism. This study show distinct expression profiles of LncRNAs between brown adipose tissue and skeletal muscle which provide information for further research on differentiation of adipocyte and transdifferentiation between BAT and skeletalmuscle that will be helpful to find a new therapeutic target for combatting obesity.
authors.affiliation: Nanjing Maternal and Child Health Care Institute, Nanjing Maternal and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China; Department of Pediatric Center, The Second Affiliated Hospital of Nanjing Medical University, Nanjing 210003, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Nanjing Maternal and Child Health Care Institute, Nanjing Maternal and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xianwei
authors.initials: X
authors.lastname: Cui
authors.affiliation: Nanjing Maternal and Child Health Care Institute, Nanjing Maternal and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Nanjing Maternal and Child Health Care Institute, Nanjing Maternal and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China.
authors.forename: Lingxia
authors.initials: L
authors.lastname: Pang
authors.affiliation: Department of Pediatric Center, The Second Affiliated Hospital of Nanjing Medical University, Nanjing 210003, China.
authors.forename: Aiqing
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Nanjing Maternal and Child Health Care Institute, Nanjing Maternal and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Fu
authors.affiliation: The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang 453003, China.
authors.forename: Jiantao
authors.initials: J
authors.lastname: Chen
authors.affiliation: Nanjing Maternal and Child Health Care Institute, Nanjing Maternal and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xirong
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Pediatric Center, The Second Affiliated Hospital of Nanjing Medical University, Nanjing 210003, China. Electronic address: doctor_cn@163.com.
authors.forename: Weihua
authors.initials: W
authors.lastname: Gan
authors.affiliation: Nanjing Maternal and Child Health Care Institute, Nanjing Maternal and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China. Electronic address: chenboji@njmu.edu.cn.
authors.forename: Chenbo
authors.initials: C
authors.lastname: Ji
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D019295:Computational Biology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D063990:Gene Ontology; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics
chemical_list: D062085:RNA, Long Noncoding
keywords: Brown adipose tissue; Long non-coding RNA; Microarray; Skeletalmuscle