title: MicroRNA319a-targeted Brassica rapa ssp. pekinensis TCP genes modulate head shape in chinese cabbage by differential cell division arrest in leaf regions.
abstract: Leafy heads of cabbage (Brassica oleracea), Chinese cabbage (Brassica rapa), and lettuce (Lactuca sativa) are composed of extremely incurved leaves. The shape of these heads often dictates the quality, and thus the commercial value, of these crops. Using quantitative trait locus mapping of head traits within a population of 150 recombinant inbred lines of Chinese cabbage, we investigated the relationship between expression levels of microRNA-targeted Brassica rapa ssp. pekinensis TEOSINTE BRANCHED1, cycloidea, and PCF transcription factor4 (BrpTCP4) genes and head shape. Here, we demonstrate that a cylindrical head shape is associated with relatively low BrpTCP4-1 expression, whereas a round head shape is associated with high BrpTCP4-1 expression. In the round-type Chinese cabbage, microRNA319 (miR319) accumulation and BrpTCP4-1 expression decrease from the apical to central regions of leaves. Overexpression of BrpMIR319a2 reduced the expression levels of BrpTCP4 and resulted in an even distribution of BrpTCP4 transcripts within all leaf regions. Changes in temporal and spatial patterns of BrpTCP4 expression appear to be associated with excess growth of both apical and interveinal regions, straightened leaf tips, and a transition from the round to the cylindrical head shape. These results suggest that the miR319a-targeted BrpTCP gene regulates the round shape of leafy heads via differential cell division arrest in leaf regions. Therefore, the manipulation of miR319a and BrpTCP4 genes is a potentially important tool for use in the genetic improvement of head shape in these crops.
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Mao
authors.forename: Feijie
authors.initials: F
authors.lastname: Wu
authors.forename: Xiang
authors.initials: X
authors.lastname: Yu
authors.forename: Jinjuan
authors.initials: J
authors.lastname: Bai
authors.forename: Weili
authors.initials: W
authors.lastname: Zhong
authors.forename: Yuke
authors.initials: Y
authors.lastname: He
mesh_terms: D001483:Base Sequence; D001937:Brassica / Q000033:anatomy & histology* / Q000166:cytology / Q000235:genetics* / Q000648:ultrastructure; D002455:Cell Division / Q000235:genetics*; D048429:Cell Size; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D017343:Genes, Plant / Q000235:genetics*; D007178:Inbreeding; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009929:Organ Size; D010641:Phenotype; D010802:Phylogeny; D019441:Plant Epidermis / Q000166:cytology; D018515:Plant Leaves / Q000033:anatomy & histology / Q000166:cytology* / Q000235:genetics* / Q000648:ultrastructure; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic / Q000235:genetics; D013997:Time Factors
chemical_list: D035683:MicroRNAs; D010940:Plant Proteins; D012333:RNA, Messenger