title: Influence of the physical and chemical properties of magnetic nanoparticles on their performance in a chemiluminescence immunoassay.
abstract: OBJECTIVE
  Magnetic nanoparticles (MNPs) are important carriers in immunoassays. In this study, we investigated the influence of the physical and chemical properties of MNPs on their performance in a detection process.
  
  
  METHODS
  A comparative study of the properties of two MNPs with sizes of 200nm and 1μm (MNP-200nm and MNP-1μm, respectively) was conducted using the following four aspects: nonspecific adsorption to proteins (IgG was used as a model protein), influence of magnetic nanoparticles on the chemiluminescence signal, response speed to an external magnetic field, and intensity of the detection signal.
  
  
  RESULTS
  MNP-1μm exhibited lower nonspecific adsorption to IgG in serum, a weaker interference with the chemiluminescence signal, and a higher response speed to the external magnetic field than the same amount of MNP-200nm. An automated chemiluminescence immunoassay system based on MNP-1μm was also established.
  
  
  CONCLUSIONS
  MNP-1μm acts as an excellent carrier in an automated chemiluminescence immunoassay system for the analysis of serum samples from clinical patients.
authors.affiliation: Division of Cardiology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, PR China; Nano Biomedical Research Center, School of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, PR China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Dai
authors.affiliation: Nano Biomedical Research Center, School of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, PR China. Electronic address: xuhong@sjtu.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.affiliation: Division of Cardiology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, PR China.
authors.forename: Yongguang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Nano Biomedical Research Center, School of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, PR China.
authors.forename: Hongchen
authors.initials: H
authors.lastname: Gu
authors.affiliation: Division of Cardiology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, PR China. Electronic address: weim.sjtu6h@hotmail.com.
authors.forename: Meng
authors.initials: M
authors.lastname: Wei
mesh_terms: D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D007074:Immunoglobulin G; D058185:Magnetite Nanoparticles
keywords: Chemiluminescence immunoassay; Detection performance; Magnetic nanoparticle; Myoglobin; Physicochemical property