title: Evaluation of matrix solid-phase dispersion (MSPD) extraction for multi-fenicols determination in shrimp and fish by liquid chromatography-electrospray ionisation tandem mass spectrometry.
abstract: A quantitative LC-MS/MS method was developed for the determination of chloramphenicol (CAP), thiamphenicol (TAP), florfenicol (FF) and florfenicol amine (FFA) in shrimp and fish. This rapid simple and effective extraction method was based on matrix solid-phase dispersion (MSPD). The best results were obtained using C18 as dispersant sorbent. The correlation coefficient (r) with each matrix-matched calibration curve is higher than 0.999 at the range of 0.05-0.8μg/kg for CAP and FF, 0.1-1.6μg/kg for FFA and TAP. CCα and CCβ of the fenicols upon the method were ranged from 0.01 to 0.09μg/kg and 0.04 to 0.25μg/kg respectively. In the fortified levels recoveries of the four compounds ranged from 83.8% to 98.8% with RSDs lower than 13.7%. The proposed method has been applied successfully to the analysis of CAP, TAP, FF and FFA in shrimp and fish samples, which demonstrates that this method is fast, sensitive, reliable and environmental friendly.
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues (HZAU)/MOA Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Tao
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues (HZAU)/MOA Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Fangwei
authors.initials: F
authors.lastname: Zhu
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues (HZAU)/MOA Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Chen
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues (HZAU)/MOA Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Wei
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues (HZAU)/MOA Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yuanhu
authors.initials: Y
authors.lastname: Pan
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues (HZAU)/MOA Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues (HZAU)/MOA Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Zhenli
authors.initials: Z
authors.lastname: Liu
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues (HZAU)/MOA Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Lingli
authors.initials: L
authors.lastname: Huang
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues (HZAU)/MOA Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yulian
authors.initials: Y
authors.lastname: Wang
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues (HZAU)/MOA Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: yuan5802@mail.hzau.edu.cn.
authors.forename: Zonghui
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000302:isolation & purification*; D002701:Chloramphenicol / Q000032:analysis / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D003445:Crustacea / Q000737:chemistry; D005399:Fishes; D017747:Seafood / Q000032:analysis*; D012758:Shellfish / Q000032:analysis; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D002701:Chloramphenicol; C083312:florfenicol amine; C035534:florfenicol; D013839:Thiamphenicol
keywords: Experimental design method; Fenicols; LC–MS/MS; Matrix solid-phase dispersion; Multi-residue