title: Genetic analysis of benzothiophene biodesulfurization pathway of Gordonia terrae strain C-6.
abstract: Sulfur can be removed from benzothiophene (BT) by some bacteria without breaking carbon-carbon bonds. However, a clear mechanism for BT desulfurization and its genetic components have not been reported in literatures so far. In this study, we used comparative transcriptomics to study differential expression of genes in Gordonia terrae C-6 cultured with BT or sodium sulfate as the sole source of sulfur. We found that 135 genes were up-regulated with BT relative to sodium sulfate as the sole sulfur source. Many of these genes encode flavin-dependent monooxygenases, alkane sulfonate monooxygenases and desulfinase, which perform similar functions to those involved in the 4S pathway of dibenzothiophene (DBT) biodesulfurization. Three of the genes were found to be located in the same operon, designated bdsABC. Cell extracts of pET28a-bdsABC transfected E. coli Rosetta (DE3) converted BT to a phenolic compound, identified as o-hydroxystyrene. These results advance our understanding of enzymes involved in the BT biodesulfurization pathway.
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, P. R. China ; TEDA School of Biological Sciences and Biotechnology, Nankai University, Tianjin, P. R. China ; Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, P. R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Ma
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, P. R. China.
authors.forename: Kehui
authors.initials: K
authors.lastname: Lian
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, P. R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, P. R. China.
authors.forename: Huimei
authors.initials: H
authors.lastname: Tian
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, P. R. China.
authors.forename: Kaihua
authors.initials: K
authors.lastname: Ji
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, P. R. China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Li
mesh_terms: D000192:Actinomycetales / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D049109:Cell Proliferation; D003198:Computer Simulation; D046508:Culture Techniques; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D013455:Sulfur / Q000378:metabolism*; D013876:Thiophenes / Q000378:metabolism*
chemical_list: D013876:Thiophenes; C088015:benzothiophene; D013455:Sulfur