title: Hepatitis C genotype and associated risks factors of patients at University Kebangsaan Malaysia Medical Centre.
abstract: OBJECTIVE
  Hepatitis C virus (HCV) genotyping is important for treatment and epidemiological purposes. The objective was to determine HCV genotype and their associations with certain risk factors at University Kebangsaan Malaysia Medical Centre (UKMMC).
  
  
  METHODS
  A total of 89 samples were collected from December 2009 to January 2011. Demographic data of patients were collected from medical record. Reverse Transcriptase Polymerase chain reaction (RT PCR) was performed and sixty-four samples yielded positive for HCV. Sequencing was performed and analyzed based on sequence information in GenBank. Statistical analysis were done using SPSS version 15. Results : HCV genotype 3 (73%) was the most frequent genotype, followed by genotype 1(27%). The distribution of HCV genotype/ subtype was as follows: 3a (64.8%), 1a (13.5%), 1 (10.8%), 3 (8.1%) and 1b (2.7%).
  
  
  CONCLUSIONS
  HCV subtypes 3a, 1a, and 1b were identified in patients at UKMMC, Malaysia with subtype 3a being the most prevalent. No significant association was found between HCV genotypes and patients' demographic data.
authors.affiliation: N.A. Mohamed, Faculty of Medicine & Health Sciences, University Sains Islam Malaysia, Kuala Lumpur, Malaysia.
authors.forename: N A
authors.initials: NA
authors.lastname: Mohamed
authors.affiliation: Z. Zainol Rashid, K.K. Wong, Department of Medical Microbiology & Immunology, Faculty of Medicine, Department of Medical Microbiology & Immunology, University Kebangsaan Malaysia Medical Centre (UKMMC), Kuala Lumpur, Malaysia.
authors.forename: Z
authors.initials: Z
authors.lastname: Zainol Rashid
authors.affiliation: K.K. Wong, Department of Medical Microbiology & Immunology, Faculty of Medicine, Department of Medical Microbiology & Immunology, University Kebangsaan Malaysia Medical Centre (UKMMC), Kuala Lumpur, Malaysia.
authors.forename: K K
authors.initials: KK
authors.lastname: Wong
authors.affiliation: Abdullah S.A, Department of Medicine, Department of Medical Microbiology & Immunology, Faculty of Medicine, Department of Medical Microbiology & Immunology, University Kebangsaan Malaysia Medical Centre (UKMMC), Kuala Lumpur, Malaysia.
authors.forename: Abdullah
authors.initials: A
authors.lastname: S A
authors.affiliation: M. M. Rahman, Department of Medical Microbiology & Immunology, Faculty of Medicine, Department of Medical Microbiology & Immunology, University Kebangsaan Malaysia Medical Centre (UKMMC), Kuala Lumpur, Malaysia.
authors.forename: M M
authors.initials: MM
authors.lastname: Rahman
keywords: HCV genotype; HCV subtype; Phylogenetic analysis; RT-PCR; Risk factors