title: Aqueous extract of Crataegus azarolus protects against DNA damage in human lymphoblast Cell K562 and enhances antioxidant activity.
abstract: The present study was carried out to characterize the cellular antioxidant effect of the aqueous extract of Crataegus azarolus and its antigenotoxic potential using human myelogenous cells, K562. The antioxidant capacity of this extract was evaluated by determining its cellular antioxidant activity (CAA) in K562 cells. Also, preceding antigenotoxicity assessment, its eventual genotoxicity property was investigated by evaluating its capacity to induce the DNA degradation of treated cell nuclei. As no genotoxicity was detected at different exposure times, its ability to protect cell DNA against H2O2 oxidative effect was investigated, using the "comet assay." It appears that 800 μg/mL of extract inhibited the genotoxicity induced by H2O2 with a rate of 41.30 %, after 4 h of incubation. In addition, this extract revealed a significant cellular antioxidant capacity against the reactive oxygen species in K562 cells.
authors.affiliation: Laboratoire de Biologie Cellulaire et Moléculaire, Faculté de Médecine Dentaire, Université de Monastir, Rue Avicenne, 5000, Monastir, Tunisia.
authors.forename: Nadia
authors.initials: N
authors.lastname: Mustapha
authors.forename: Inès
authors.initials: I
authors.lastname: Bouhlel
authors.forename: Fadwa
authors.initials: F
authors.lastname: Chaabane
authors.forename: Imèn Mokdad
authors.initials: IM
authors.lastname: Bzéouich
authors.forename: Kamel
authors.initials: K
authors.lastname: Ghedira
authors.forename: Thierry
authors.initials: T
authors.lastname: Hennebelle
authors.forename: Leila
authors.initials: L
authors.lastname: Chekir-Ghedira
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D027825:Crataegus / Q000737:chemistry*; D004249:DNA Damage / Q000187:drug effects; D006801:Humans; D020014:K562 Cells; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts