title: Genetic circuitry modulating notch signals through endosomal trafficking.
abstract: Genetic modifier screens offer a powerful, indeed a uniquely powerful tool for the analysis and identification of elements capable of modulating specific cellular functions in development. Here, we describe the methodology that allowed us to explore the genetic circuitry that affects a Notch mutant phenotype caused by the abnormal endosomal trafficking of the Notch receptor. Endosomal trafficking events are increasingly appreciated to play a major role in controlling Notch signaling in development.
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Hori
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Anindya
authors.initials: A
authors.lastname: Sen
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA. Electronic address: artavanis@hms.harvard.edu.
authors.forename: Spyros
authors.initials: S
authors.lastname: Artavanis-Tsakonas
mesh_terms: D000483:Alleles; D000818:Animals; D019390:Arrestins / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D005820:Genetic Testing; D049750:Genome, Insect; D005838:Genotype; D060227:Imaginal Discs / Q000166:cytology / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D021381:Protein Transport; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics*; D054875:Ubiquitination; D014921:Wings, Animal / Q000254:growth & development / Q000378:metabolism / Q000473:pathology
chemical_list: D019390:Arrestins; C086679:DX protein, Drosophila; D029721:Drosophila Proteins; D008565:Membrane Proteins; C497759:N protein, Drosophila; D051880:Receptors, Notch; C412169:krz protein, Drosophila
keywords: Endosome; Genetic screen; Membrane trafficking; Notch signal