title: A mildly inducible and selective cross-link methodology for RNA duplexes.
abstract: We here report on the furan oxidation methodology for interstrand cross-linking of RNA duplexes, which have a different structure and are more stiff, reactive and labile than their DNA counterparts. Through this mildly inducible approach, natural unmodified RNA can be selectively cross-linked in high yield. The method therefore has direct applications in the increasing number of RNA based technologies.
authors.affiliation: Organic and Biomimetic Chemistry Research group, Department of Organic Chemistry, Gent University, Krijgslaan 281-S4, 9000 Gent, Belgium. annemieke.madder@ugent.be.
authors.forename: L L G
authors.initials: LL
authors.lastname: Carrette
authors.forename: E
authors.initials: E
authors.lastname: Gyssels
authors.forename: J
authors.initials: J
authors.lastname: Loncke
authors.forename: A
authors.initials: A
authors.lastname: Madder
mesh_terms: D003432:Cross-Linking Reagents / Q000138:chemical synthesis / Q000737:chemistry*; D005663:Furans / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D012313:RNA / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; D005663:Furans; D012313:RNA