title: Identification and expression analysis of primary auxin-responsive Aux/IAA gene family in cucumber (Cucumis sativus).
abstract: Aux/IAA is an important gene family involved in many aspects of growth and development. Aux/IAA proteins are short-lived nuclear proteins that are induced primarily by various phytohormones. In this study, 29 Aux/IAA family genes (CsIAA01-CsIAA29) were identified and characterized in cucumber, including gene structures, phylogenetic relationships, conserved protein motifs and chromosomal locations. These genes show distinct organizational patterns of their putative motifs. The distributions of the genes vary: except for five CsIAA genes in cucumber that were not located, seven CsIAA genes were found on scaffold, while the other 17 CsIAA genes were distributed on seven other chromosomes. Based on a phylogenetic analysis of the Aux/IAA protein sequences from cucumber, Arabidopsis and other plants, the Aux/IAA genes in cucumber were categorized into seven subfamilies. To investigate whether the expression of CsIAA genes is associated with auxin induction, their transcript levels were monitored in seedlings treated with IAA (indole-3-acetic acid), and their expression patterns were analysed by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). The results showed that 11/29 CsIAA genes were expressed in leaves whether treated with IAA or not and the time course of processing and compared with the control, five CsIAA genes showed low expression only after 60 min treatment with IAA, while 11 genes showed no expression. These results provide useful information for further functional analysis of Aux/IAA gene family in cucumber.
authors.affiliation: Anhui Province Key Laboratory of Crop Biology, School of Life Science, Anhui Agricultural University, Hefei 230036, People's Republic of China. dfgan1@163.com.
authors.forename: Defang
authors.initials: D
authors.lastname: Gan
authors.forename: Dan
authors.initials: D
authors.lastname: Zhuang
authors.forename: Fei
authors.initials: F
authors.lastname: Ding
authors.forename: Zhenzhou
authors.initials: Z
authors.lastname: Yu
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D017124:Conserved Sequence; D018553:Cucumis sativus / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D017343:Genes, Plant; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D059467:Transcriptome
chemical_list: D009687:Nuclear Proteins; D010940:Plant Proteins; D012333:RNA, Messenger