title: Abnormal expression of CDK11p58 in prostate cancer.
abstract: BACKGROUND
  CDK11p58 is one of the large families of p34cdc2-related kinases whose functions are linked with cell cycle progression, tumorigenesis and apoptotic signaling. Our previous investigation demonstrated that CDK11p58 repressed androgen receptor (AR) transcriptional activity and was involved in the negative regulation of AR function.
  
  
  METHODS
  CDK11p58 expression was examined in the prostate cancer tissues and adjacent tissues by IHC and qRT-PCR. Cell apoptosis was detected by flow cytometry. The metastasis of cancer cells was evaluated by the Transwell Assay. Finally we further investigated the underlying molecular mechanisms by examining expression levels of relevant proteins using western blot analysis.
  
  
  RESULTS
  We found that both RNA and protein expression of CDK11p58 were low in prostate cancer tissues compared with its adjacent noncancerous tissues. CDK11p58 promoted the prostate cancer cell apoptosis and inhibited its metastasis in a kinase dependent way. And finally CDK11p58 could inhibit the metastasis of AR positive prostate cancer cells through inhibition of integrin β3 and MMP2.
  
  
  CONCLUSIONS
  These data indicate that CDK11p58 is an anti-metastasis gene product in prostate cancer.
authors.forename: Yayun
authors.initials: Y
authors.lastname: Chi
authors.forename: Lisha
authors.initials: L
authors.lastname: Wang
authors.forename: Xiuying
authors.initials: X
authors.lastname: Xiao
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
authors.forename: Yiqin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai 200032, China. xyzhou100@sina.cn.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhou