title: Serum concentrations of insulin-like growth factor-binding protein 5 in Crohn's disease.
abstract: OBJECTIVE
  To investigate serum insulin-like growth factor-binding protein 5 (IGFBP-5) levels and intestinal IGFBP-5 expression in patients with Crohn's disease (CD).
  
  
  METHODS
  We analyzed the serum concentrations and intestinal expression of IGFBP-5 in 42 patients with CD, of whom 26 had endoscopically or radiologically proven stricture formation. Nine of the 42 patients had active disease, with a Crohn's disease activity index > 150. Serum IGFBP-5 levels were analyzed in 20 healthy controls matched by sex and age to the CD patients. Serum IGFBP-5 was measured using an enzyme-linked immunosorbent assay. Intestinal tissue was obtained from patients through endoscopic biopsies. IGFBP-5 expression was detected using immunohistochemistry and was scored semiquantitatively.
  
  
  RESULTS
  The median serum IGFBP-5 concentrations of CD patients were significantly lower compared with healthy controls [median 7.2 (IQR: 5.5-11.3) ng/mL vs 11.3 (8.0-44.6) ng/mL, P < 0.001]. There was no significant difference between median serum IGFBP-5 levels in CD patients with or without stricture formation [6.9 (5.5-11.3) ng/mL vs 7.8 (5.3-10.1) ng/mL, P = 0.815]. The serum IGFBP-5 levels were not significantly different between patients with active disease and inactive disease [7.2 (6.5-7.6) ng/mL vs 7.2 (5.5-11.3) ng/mL, P = 0.890]. However, a significant correlation was observed between serum IGFBP-5 levels and platelet count (PLT) (r = 0.319, P = 0.0395). No significant correlation was found between tissue IGFBP-5 immunohistochemical staining intensity scores and serum IGFBP-5 levels. No significant difference was found when comparing the serum IGFBP-5 levels among the patients with different tissue IGFBP-5 staining scores (absent/very weak, weak, moderate or strong). There was a significant correlation between tissue IGFBP-5 staining scores and white blood cell count (r = 0.391, P = 0.01) and PLT (r = 0.356, P = 0.021).
  
  
  CONCLUSIONS
  Our results indicate that serum IGFBP-5 concentrations were lower in CD patients compared to healthy controls regardless of disease activity or the presence of stricture formation.
authors.affiliation: Gupse Adali, Elif Yorulmaz, Celal Ulasoglu, Yasar Colak, Ilyas Tuncer, Department of Gastroenterology, Goztepe Training and Research Hospital, Istanbul Medeniyet University, Doktor Erkin Caddesi Kadikoy, Istanbul 34730, Turkey.
authors.forename: Gupse
authors.initials: G
authors.lastname: Adali
authors.affiliation: Gupse Adali, Elif Yorulmaz, Celal Ulasoglu, Yasar Colak, Ilyas Tuncer, Department of Gastroenterology, Goztepe Training and Research Hospital, Istanbul Medeniyet University, Doktor Erkin Caddesi Kadikoy, Istanbul 34730, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Yorulmaz
authors.affiliation: Gupse Adali, Elif Yorulmaz, Celal Ulasoglu, Yasar Colak, Ilyas Tuncer, Department of Gastroenterology, Goztepe Training and Research Hospital, Istanbul Medeniyet University, Doktor Erkin Caddesi Kadikoy, Istanbul 34730, Turkey.
authors.forename: Seyma
authors.initials: S
authors.lastname: Ozkanli
authors.affiliation: Gupse Adali, Elif Yorulmaz, Celal Ulasoglu, Yasar Colak, Ilyas Tuncer, Department of Gastroenterology, Goztepe Training and Research Hospital, Istanbul Medeniyet University, Doktor Erkin Caddesi Kadikoy, Istanbul 34730, Turkey.
authors.forename: Celal
authors.initials: C
authors.lastname: Ulasoglu
authors.affiliation: Gupse Adali, Elif Yorulmaz, Celal Ulasoglu, Yasar Colak, Ilyas Tuncer, Department of Gastroenterology, Goztepe Training and Research Hospital, Istanbul Medeniyet University, Doktor Erkin Caddesi Kadikoy, Istanbul 34730, Turkey.
authors.forename: Baris
authors.initials: B
authors.lastname: Bayraktar
authors.affiliation: Gupse Adali, Elif Yorulmaz, Celal Ulasoglu, Yasar Colak, Ilyas Tuncer, Department of Gastroenterology, Goztepe Training and Research Hospital, Istanbul Medeniyet University, Doktor Erkin Caddesi Kadikoy, Istanbul 34730, Turkey.
authors.forename: Alev
authors.initials: A
authors.lastname: Orhun
authors.affiliation: Gupse Adali, Elif Yorulmaz, Celal Ulasoglu, Yasar Colak, Ilyas Tuncer, Department of Gastroenterology, Goztepe Training and Research Hospital, Istanbul Medeniyet University, Doktor Erkin Caddesi Kadikoy, Istanbul 34730, Turkey.
authors.forename: Yasar
authors.initials: Y
authors.lastname: Colak
authors.affiliation: Gupse Adali, Elif Yorulmaz, Celal Ulasoglu, Yasar Colak, Ilyas Tuncer, Department of Gastroenterology, Goztepe Training and Research Hospital, Istanbul Medeniyet University, Doktor Erkin Caddesi Kadikoy, Istanbul 34730, Turkey.
authors.forename: Ilyas
authors.initials: I
authors.lastname: Tuncer
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D001706:Biopsy; D016022:Case-Control Studies; D003424:Crohn Disease / Q000097:blood* / Q000175:diagnosis; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D018975:Insulin-Like Growth Factor Binding Protein 5 / Q000097:blood*; D007422:Intestines / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011379:Prognosis; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D015415:Biomarkers; D018975:Insulin-Like Growth Factor Binding Protein 5
keywords: Crohn’s disease; Enzyme-linked immunosorbent assay; Immunohistochemistry; Insulin-like growth factor-binding protein 5; Stricture