title: Facile synthesis of gold nanohexagons on graphene templates in Raman spectroscopy for biosensing cancer and cancer stem cells.
abstract: Several surface enhanced Raman spectroscopy (SERS) substrates were prepared based on in situ nucleation of gold nanohexagons (Au) on graphene (G) nanosheets (Au@G), G, Au nanoparticles and Au conjugated G nanomaterials. These were applied to enhance Raman scattering and to differentiate human breast normal, cancer and cancer stem cells. These SERS substrates at concentrations of 100 µg/1 × 10(4) cells led to 5.4 fold increase in detecting breast cancer cells (BCCs) and 4.8 fold of sensitivity for detecting breast cancer stem cells (BCSCs) and they were able to identify and differentiate between normal cells, cancer cells and cancer stem cells. These approaches are rapid, simple and reliable for healthy normal cells, cancer cells and cancer stem cell detection which have a huge potential for cancer research for medical or biomedicine applications.
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, 80424, Taiwan; Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, 80424, Taiwan.
authors.forename: M
authors.initials: M
authors.lastname: Manikandan
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, 80424, Taiwan; Department of Chemistry, Assuit University, Assuit 71515, Egypt.
authors.forename: Hani
authors.initials: H
authors.lastname: Nasser Abdelhamid
authors.affiliation: Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, 80424, Taiwan.
authors.forename: Abou
authors.initials: A
authors.lastname: Talib
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, 80424, Taiwan; School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 800, Taiwan; Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, 80424, Taiwan; Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, 80424, Taiwan; Institute of Medical Science and Technology, National Sun Yat-Sen University, 80424, Taiwan. Electronic address: hwu@faculty.nsysu.edu.tw.
authors.forename: Hui-Fen
authors.initials: HF
authors.lastname: Wu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D001943:Breast Neoplasms / Q000175:diagnosis*; D003460:Crystallization / Q000379:methods; D003937:Diagnosis, Differential; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D014411:Neoplastic Stem Cells / Q000473:pathology*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014407:Tumor Cells, Cultured
chemical_list: D006046:Gold; D006108:Graphite
keywords: Au nanoparticles; Cancer cells; Cancer stem cells; Graphene; Surface enhanced Raman spectroscopy