title: Femtogram detection of cytokines in a direct dot-blot assay using SERS microspectroscopy and hydrophilically stabilized Au-Ag nanoshells.
abstract: Rapid parallel detection of two cytokines (IL-6 and IL-8) with femtogram sensitivity in a simple direct dot-blot assay is demonstrated. The microspectroscopic SERS acquisition scheme employs rationally designed, hydrophilically stabilized Au-Ag nanoshells as SERS labels, which are optimized for signal enhancement upon red laser excitation.
authors.affiliation: Faculty of Chemistry and Center for Nanointegration Duisburg-Essen (CENIDE), University of Duisburg-Essen, Universitaetsstr. 5, D-45141 Essen, Germany. sebastian.schluecker@uni-due.de.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Wang
authors.forename: Mohammad
authors.initials: M
authors.lastname: Salehi
authors.forename: Max
authors.initials: M
authors.lastname: Schütz
authors.forename: Sebastian
authors.initials: S
authors.lastname: Schlücker
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D000941:Antigens / Q000032:analysis / Q000276:immunology; D006046:Gold / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D015151:Immunoblotting; D015850:Interleukin-6 / Q000032:analysis* / Q000276:immunology; D016209:Interleukin-8 / Q000032:analysis* / Q000276:immunology; D057145:Nanoshells / Q000737:chemistry*; D012710:Serum Albumin, Bovine; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
chemical_list: D000906:Antibodies; D000941:Antigens; D015850:Interleukin-6; D016209:Interleukin-8; D012710:Serum Albumin, Bovine; D012834:Silver; D006046:Gold