title: Tuning the material-cytoskeleton crosstalk via nanoconfinement of focal adhesions.
abstract: Material features proved to exert a potent influence on cell behaviour in terms of adhesion, migration and differentiation. In particular, biophysical and biochemical signals on material surfaces are able to affect focal adhesion distribution and cytoskeletal assemblies, which are known to regulate signalling pathways that ultimately influence cell fate and functions. However, a general, unifying model that correlates cytoskeletal-generated forces with genetic events has yet to be developed. Therefore, it is crucial to gain a better insight into the material-cytoskeleton crosstalk in order to design and fabricate biomaterials able to govern cell fate more accurately. In this work, we demonstrate that confining focal adhesion distribution and growth dramatically alters the cytoskeleton's structures and dynamics, which in turn dictate cellular and nuclear shape and polarization. MC3T3 preosteoblasts were cultivated on nanograted polydimethylsiloxane substrates and a thorough quantification - in static and dynamic modes - of the morphological and structural features of focal adhesions and cytoskeleton was performed. Nanoengineered surfaces provided well-defined zones for focal adhesions to form and grow. Unique cytoskeletal structures spontaneously assembled when focal adhesions were confined and, in fact, they proved to be very effective in deforming the nuclei. The results here presented provide elements to engineer surfaces apt to guide and control cell behaviour through the material-cytoskeleton-nucleus axis.
authors.affiliation: Center for Advanced Biomaterials for Healthcare@CRIB, Istituto Italiano di Tecnologia, Naples 80125, Italy; Department of Chemical, Materials and Industrial Production Engineering, University of Naples Federico II, Naples 80125, Italy; Interdisciplinary Research Center on Biomaterials, University of Naples Federico II, Naples 80125, Italy.
authors.forename: Carlo F
authors.initials: CF
authors.lastname: Natale
authors.affiliation: Center for Advanced Biomaterials for Healthcare@CRIB, Istituto Italiano di Tecnologia, Naples 80125, Italy; Department of Chemical, Materials and Industrial Production Engineering, University of Naples Federico II, Naples 80125, Italy; Interdisciplinary Research Center on Biomaterials, University of Naples Federico II, Naples 80125, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Ventre
authors.affiliation: Center for Advanced Biomaterials for Healthcare@CRIB, Istituto Italiano di Tecnologia, Naples 80125, Italy; Department of Chemical, Materials and Industrial Production Engineering, University of Naples Federico II, Naples 80125, Italy; Interdisciplinary Research Center on Biomaterials, University of Naples Federico II, Naples 80125, Italy. Electronic address: nettipa@unina.it.
authors.forename: Paolo A
authors.initials: PA
authors.lastname: Netti
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002460:Cell Line; D053144:Cell Nucleus Shape / Q000187:drug effects; D016764:Cell Polarity / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism*; D022001:Focal Adhesions / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D053758:Nanoparticles / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D022502:Stress Fibers / Q000187:drug effects / Q000378:metabolism; D059008:Time-Lapse Imaging
chemical_list: D000199:Actins; D001672:Biocompatible Materials
keywords: Adhesive nanopatterns; Cell adhesion; Cell morphology; Mechanotransduction; Polydimethylsiloxane; Progenitor cell