title: Allele-specific effects of PDF2 on floral morphology in Arabidopsis thaliana.
abstract: The class IV Homeodomain-leucine zipper (HD-ZIP IV) gene family includes several genes that are functionally significant in epidermal development. Our recent study revealed that double mutants of the epidermis-expressed HD-ZIP IV members, PROTODERMAL FACTOR2 (PDF2) in combination with some HOMEODOMAIN GLABROUS (HDG, pronounced "hedge") genes, affect stamen development and specification of petal and stamen identity, possibly in a non cell-autonomous manner. However, the effect of the pdf2 mutations on the floral development was largely different depending on T-DNA insertion locations: pdf2-1 hdg flowers exhibited homeotic conversion of petals and stamens, while pdf2-2 hdg flowers had only a reduced number of stamens. Here, we used 2 additional pdf2 alleles to make double mutants and found that their floral phenotypes were rather similar to those of pdf2-2 hdg. The allele-specific effect caused by pdf2-1, which carries a T-DNA in a steroidogenic acute regulatory protein-related lipid transfer (START) domain-encoding region, suggests the importance of the START domain in proper function of HD-ZIP IV proteins.
authors.affiliation: Department of Biological Science; Graduate School of Science; The University of Tokyo; Tokyo, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Kamata
authors.affiliation: Division of Bioscience; Graduate School of Natural Science and Technology; Okayama University; Okayama, Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Sugihara
authors.affiliation: Department of Biological Science; Graduate School of Science; The University of Tokyo; Tokyo, Japan.
authors.forename: Yoshibumi
authors.initials: Y
authors.lastname: Komeda
authors.affiliation: Division of Bioscience; Graduate School of Natural Science and Technology; Okayama University; Okayama, Japan.
authors.forename: Taku
authors.initials: T
authors.lastname: Takahashi
mesh_terms: D000483:Alleles; D017360:Arabidopsis / Q000033:anatomy & histology* / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D035264:Flowers / Q000033:anatomy & histology* / Q000235:genetics*; D017343:Genes, Plant; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D010641:Phenotype
chemical_list: D029681:Arabidopsis Proteins; D018398:Homeodomain Proteins; C472305:PDF2 protein, Arabidopsis
keywords: Arabidopsis thaliana; HOMEODOMAIN GLABROUS(HDG); PROTODERMAL FACTOR2(PDF2); epidermis; floral development; steroidogenic acute regulatory protein-related lipid transfer (START) domain