title: [Comparison on content of ligustilides in different danggui samples].
abstract: Bioactivity of Danggui is linked to the content of ligustilide, but the relationship between ligustilide with herb shape, cultivating areas and plant species is still unknown. The relationship was investigated by quantifying on the amounts of Z-ligustilide and E-ligustilide by HPLC-DAD-MS method, and then comparing the content of ligustilides (the sum of Z-ligustilide and E-ligustilide) among forty-four various "Danggui" samples containing thirty Chinese Danggui (CDG), six Japanese Danggui (JDG), four Korea Danggui (KDG) and four European Danggui (EDG). Results showed that the content of ligustilides in CDG samples (Angelica sinensis) was in the range of 5.63-24.53 mg x g(-1) with the mean of 11.02 mg x g(-1) (n = 30). Ligustilides amounts were varied among samples cultivated in different areas in China, i. e. 13.90 mg x g(-1) (n = 6) in Yannan, 12.51 mg x g(-1) (n = 6) in Sichuan and 10.04 mg x g(-1) (n = 13) in Gansu. It was also found that ligustilides content was related to the shape, color and fragrance of herb, e. g. the relative larger amount of ligustilides was in the small main root, long rootlet and perfumed sample. Further, ligustilides contents were estimated to be 1.00 mg x g(-1) (n = 6) in JDG samples (A. acutiloba and A. acutiloba var. sugiyamae) and 2.78 mg x g(-1) (n = 2) in EDG samples (lovage root, Levisticum officinale). However, ligustilides could not be detected in the four KDG samples (A. gigas) and two EDG samples (angelica root, A. archangelica). It has been concluded that ligustilide is significant variant among plant species, which may result in the variety of bioactivity and therapeutic effect.
authors.affiliation: Key Laboratory of the Ministry of Education in China on the Standardization of Chinese Materia Medica, 505323500@qq.com
authors.forename: Xi
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Zhang
authors.affiliation: Key Laboratory of the Ministry of Education in China on the Standardization of Chinese Materia Medica,
authors.forename: Guang-Hua
authors.initials: GH
authors.lastname: Lv
authors.affiliation: Key Laboratory of the Ministry of Education in China on the Standardization of Chinese Materia Medica,
authors.forename: Xiao-Xiao
authors.initials: XX
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Jiang
authors.affiliation: Key Laboratory of the Ministry of Education in China on the Standardization of Chinese Materia Medica,
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Jin
authors.affiliation: Sichuan Provincial Institute for Food and Drug Control, Chengdu 610097, China.
authors.forename: Zhong-Zhen
authors.initials: ZZ
authors.lastname: Zhao
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000032:analysis; D029971:Angelica sinensis / Q000737:chemistry*; D002681:China; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005843:Geography; D011786:Quality Control
chemical_list: D004365:Drugs, Chinese Herbal; C027820:ligustilide; D015107:4-Butyrolactone