title: Avidity of IgG antibodies against meningococcal serogroup a polysaccharide and correlations with bactericidal activity in sera from meningitis patients and controls from Ethiopia.
abstract: Meningococcal meningitis is a significant global health challenge, especially for sub-Saharan area: the African meningitis belt. Neisseria meningitidis of serogroup A (MenA) is responsible for the large number of epidemics that have been recorded in these countries. To determine the level of antibodies against meningococcal A polysaccharide (APS) that correlates with protection against MenA disease in the African meningitis belt, it may be important to consider antibody avidity along with quantity. In this study, two ELISA methods using the chaotropic agent ammonium thiocyanate were compared and employed to measure avidity indexes (AI) of IgG antibodies against APS in controls and in acute and convalescent sera from Ethiopian meningococcal patients. High statistical correlations between the AIs determined by the two methods were observed. The geometric mean AI (GMAI) increased with time from acute to convalescent sera indicating affinity maturation. GMAI was significantly higher in convalescent sera from the MenA patients and in sera from the controls than in acute sera from patients with meningococcal disease. A significant correlation between serum bactericidal activity titres (SBA) and concentration of IgG antibodies against APS was observed; however, our results did not indicate that determination of antibody avidities by the thiocyanate elution method gave a better correlation with SBA than anti-APS IgG concentrations determined by the standard ELISA method.
authors.affiliation: Department of Biology, Addis Ababa University, Addis Ababa, Ethiopia; Armauer Hansen Research Institute, Addis Ababa, Ethiopia.
authors.forename: M
authors.initials: M
authors.lastname: Medhane
authors.forename: G
authors.initials: G
authors.lastname: Tunheim
authors.forename: L M
authors.initials: LM
authors.lastname: Naess
authors.forename: W
authors.initials: W
authors.lastname: Mihret
authors.forename: A
authors.initials: A
authors.lastname: Bedru
authors.forename: G
authors.initials: G
authors.lastname: Norheim
authors.forename: B
authors.initials: B
authors.lastname: Petros
authors.forename: A
authors.initials: A
authors.lastname: Aseffa
authors.forename: E
authors.initials: E
authors.lastname: Rosenqvist
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D000915:Antibody Affinity; D001770:Blood Bactericidal Activity / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005002:Ethiopia; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D007223:Infant; D008585:Meningitis, Meningococcal / Q000097:blood / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology; D015203:Reproducibility of Results; D013861:Thiocyanates / Q000378:metabolism; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D011135:Polysaccharides, Bacterial; D013861:Thiocyanates; C026976:thiocyanic acid