title: Development and characterization of novel microsatellite markers in the rock bream fish Oplegnathus fasciatus.
abstract: The rock bream fish Oplegnathus fasciatus is one of the most popular aquaculture species in China. In the present study, 15 novel polymorphic microsatellite loci were isolated and characterized from a wild population of O. fasciatus from the Zhoushan coast of China. The number of alleles per polymorphic locus ranged from 4 to 9 in a sample of 30 individuals. Observed and expected heterozygosities per locus varied from 0.267 to 0.767 and from 0.395 to 0.859, respectively. Eleven of the 15 microsatellite loci conformed to Hardy-Weinberg equilibrium. No significant linkage disequilibrium between pairs of loci was detected. The present microsatellite markers could provide a useful tool for the genetic analyses of O. fasciatus.
authors.affiliation: Zhejiang Ocean University, College of Marine Science, Zhoushan, Zhejiang Province, China.
authors.forename: X X
authors.initials: XX
authors.lastname: Sun
authors.forename: D D
authors.initials: DD
authors.lastname: Xu
authors.forename: B
authors.initials: B
authors.lastname: Lou
authors.forename: S L
authors.initials: SL
authors.lastname: Li
authors.forename: W
authors.initials: W
authors.lastname: Zhan
authors.forename: Y R
authors.initials: YR
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Xin
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002681:China; D004247:DNA / Q000032:analysis; D005399:Fishes / Q000235:genetics*; D005828:Genetics, Population; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D004247:DNA