title: [The application of genotyping in the complicated ABO blood group type].
abstract: OBJECTIVE
  To investigate the application of ABO blood group genotyping in complicated ABO blood group type.
  
  
  METHODS
  Ten specimens of complicated ABO blood group were genotyped by sequence specific primer PCR (PCR -SSP), and confirmed by DNA sequencing and alignment. Six hundred and ten blood samples typed by ABO immunoassay were as control of genotyping.
  
  
  RESULTS
  Ten cases of complicated blood type were identified by high resolution PCR- SSP as rare ABO blood groups: cis-AB01 (3 cases), B(A)04 (2 cases), cisAB02, B(A)02, Bel03, Bw12 and Ael05, confirmed by DNA sequencing. Genotyping and serotype detected 610 cases ABO blood group were coincident, and the frequency of A, B, AB and O were as 28.69%, 27.54%, 8.2% and 35.57% respectively. According to the genotypes, the highest frequency subgroup was O1 (32.87%), the lowest was A2 (0.66%).
  
  
  CONCLUSIONS
  PCR -SSP could type the ABO blood group accurately, but also the sub-group of blood type. However, special designed high resolution PCR -SSP or DNA sequencing is needed to identify the complicated blood groups.
authors.affiliation: Department of Surgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
authors.forename: Chun-Wu
authors.initials: CW
authors.lastname: Zhang
authors.affiliation: Department of Surgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
authors.forename: Ben-Quan
authors.initials: BQ
authors.lastname: Wang
authors.affiliation: Department of Surgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Surgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
authors.forename: Zuo-Ting
authors.initials: ZT
authors.lastname: Xie
authors.affiliation: Department of Surgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
authors.forename: Cun-Zao
authors.initials: CZ
authors.lastname: Wu
authors.affiliation: Department of Surgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Surgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Surgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xia
authors.affiliation: Department of Surgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
authors.forename: Bi-Cheng
authors.initials: BC
authors.lastname: Chen
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics*; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D000017:ABO Blood-Group System