title: Correlation of cell surface marker expression with African swine fever virus infection.
abstract: The expression of surface markers on African swine fever virus (ASFV) infected cells was evaluated to assess their involvement in infection. Previous findings indicated CD163 expression was correlated with ASFV susceptibility. However, in this study the expression of porcine CD163 on cell lines did not increase the infection rate of these cells indicating other factors are likely to be important in determining susceptibility to infection. On adherent porcine bone marrow (pBM) cells the expression of CD45 was strongly correlated with infection. CD163 and CD203a expression correlated at intermediate levels with infection, indicating cells expressing these markers could become infected but were not preferentially infected by the virus. Most of the cells expressing MHCII were infected, indicating that they may be preferentially infected although expression of MHCII was not essential for infection and a large percentage of the infected cells were MHCII negative. CD16 showed a marked decrease in expression following infection and significantly lower levels of infected cells were shown to express CD16. Altogether these results suggest CD163 may be involved in ASFV infection but it may not be essential; the results also highlight the importance of other cell markers which requiring further investigation.
authors.affiliation: The Pirbright Institute, Ash Road, Pirbright GU24 0NF, United Kingdom.
authors.forename: Pamela
authors.initials: P
authors.lastname: Lithgow
authors.affiliation: The Pirbright Institute, Ash Road, Pirbright GU24 0NF, United Kingdom.
authors.forename: Haru
authors.initials: H
authors.lastname: Takamatsu
authors.affiliation: Royal Veterinary College, Hawkshead Lane, Hertfordshire AL9 7TA, United Kingdom.
authors.forename: Dirk
authors.initials: D
authors.lastname: Werling
authors.affiliation: The Pirbright Institute, Ash Road, Pirbright GU24 0NF, United Kingdom.
authors.forename: Linda
authors.initials: L
authors.lastname: Dixon
authors.affiliation: The Pirbright Institute, Ash Road, Pirbright GU24 0NF, United Kingdom. Electronic address: dave.chapman@pirbright.ac.uk.
authors.forename: Dave
authors.initials: D
authors.lastname: Chapman
mesh_terms: D000357:African Swine Fever / Q000175:diagnosis* / Q000378:metabolism; D000358:African Swine Fever Virus / Q000235:genetics*; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D002460:Cell Line; D002522:Chlorocebus aethiops; D005434:Flow Cytometry; D005802:Genes, MHC Class II; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D008565:Membrane Proteins / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D013552:Swine; D014709:Vero Cells; D000098902:CD163 Antigen
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D015415:Biomarkers; D000098902:CD163 Antigen; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D017493:Leukocyte Common Antigens
keywords: African swine fever virus; CD163; CD203a; CD45; MHCII; Surface markers