title: Somatic embryo-like structures of strawberry regenerated in vitro on media supplemented with 2,4-D and BAP.
abstract: Somatic embryo-like structures (SELS) were produced in vitro from leaf disk and petiole explants of two cultivars of strawberry (Fragaria x ananassa Duch) on Murashige and Skoog medium with different concentrations and combinations of 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP) and sucrose to check the embryonic nature of these structures histologically. A large number of SELS could be regenerated in both cultivars on media with 2-4 mg L(-1) 2,4-D in combination with 0.5 -1 mg L(-1) BAP and 50 g x L(-1) sucrose. Histological examination of SELS revealed the absence of a root pole. Therefore these structures cannot be strictly classified as somatic embryos. The SELS formed under the tested culture conditions represent malformed shoot-like and leaf-like structures. The importance of these results for the propagation of strawberries via somatic embryogenesis is discussed.
authors.affiliation: Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt.
authors.forename: Genesia F
authors.initials: GF
authors.lastname: Omar
authors.affiliation: Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt.
authors.forename: Fouad H
authors.initials: FH
authors.lastname: Mohamed
authors.affiliation: Department Plant Propagation, Leibniz-Institute of Vegetable and Ornamental Crops, Kühnhäuser Strasse 101, D-99090 Erfurt, Germany.
authors.forename: Klaus-Thomas
authors.initials: KT
authors.lastname: Haensch
authors.affiliation: Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt.
authors.forename: Sawsan H
authors.initials: SH
authors.lastname: Sarg
authors.affiliation: Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt.
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Morsey
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology*; D001593:Benzyl Compounds; D003470:Culture Media; D031985:Fragaria / Q000196:embryology*; D066298:In Vitro Techniques; D007701:Kinetin / Q000494:pharmacology*; D011687:Purines; D012639:Seeds / Q000187:drug effects*
chemical_list: D001593:Benzyl Compounds; D003470:Culture Media; D011687:Purines; D015084:2,4-Dichlorophenoxyacetic Acid; C480551:benzylaminopurine; D007701:Kinetin