title: Low-cost and easy-to-use "on-chip ELISA" for developing health-promoting foods.
abstract: We have determined that a biological molecule can be physically immobilized on a polymer containing an azobenzene (azopolymer) using irradiating light. We immobilized antibodies and antigens on the surface of an azopolymer coated glass slide (antibody array) to establish "on-chip ELISAs". The assays used the flat-surface of a glass slide and could be applied to both sandwich and competitive ELISAs. The sensitivity and accuracy of the on-chip ELISA were similar to a conventional ELISA using a polystyrene plate. Using the assay system, we proved that representative oxidative-biomarkers could be simultaneously measured from uL of urine. That should realize low-cost study on animal or human, and accelerate development of health-promoting foods. So, this new concept antibody array has promising applications in proteomic studies, and could be used to examine biomarkers to investigate health-promoting food.
authors.affiliation: TOYOTA Central R&D Labs., Inc., Nagoya, Aichi, Japan, hoshino77@mosk.tytlabs.co.jp.
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Hoshino
authors.forename: Osamu
authors.initials: O
authors.lastname: Watanabe
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Wu
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Takimoto
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Osawa
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology*; D000941:Antigens / Q000737:chemistry / Q000276:immunology*; D001391:Azo Compounds / Q000737:chemistry; D015415:Biomarkers / Q000652:urine; D003362:Cost-Benefit Analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D058870:Food, Organic / Q000032:analysis*; D006801:Humans; D011108:Polymers / Q000737:chemistry
chemical_list: D055910:Antibodies, Immobilized; D000941:Antigens; D001391:Azo Compounds; D015415:Biomarkers; D011108:Polymers; C009850:azobenzene