title: Inter-laboratory trial of a standardized sediment contact test with the aquatic plant Myriophyllum aquaticum (ISO 16191).
abstract: A whole-sediment toxicity test with Myriophyllum aquaticum has been developed by the German Federal Institute of Hydrology and standardized within the International Organization for Standardization (ISO; ISO 16191). An international ring-test was performed to evaluate the precision of the test method. Four sediments (artificial, natural) were tested. Test duration was 10 d, and test endpoint was inhibition of growth rate (r) based on fresh weight data. Eighteen of 21 laboratories met the validity criterion of r ≥ 0.09 d(-1) in the control. Results from 4 tests that did not conform to test-performance criteria were excluded from statistical evaluation. The inter-laboratory variability of growth rates (20.6%-25.0%) and inhibition (26.6%-39.9%) was comparable with the variability of other standardized bioassays. The mean test-internal variability of the controls was low (7% [control], 9.7% [solvent control]), yielding a high discriminatory power of the given test design (median minimum detectable differences [MDD] 13% to 15%). To ensure these MDDs, an additional validity criterion of CV ≤ 15% of the growth rate in the controls was recommended. As a positive control, 90 mg 3,5-dichlorophenol/kg sediment dry mass was tested. The range of the expected growth inhibition was proposed to be 35 ± 15%. The ring test results demonstrated the reliability of the ISO 16191 toxicity test and its suitability as a tool to assess the toxicity of sediment and dredged material.
authors.affiliation: Federal Institute of Hydrology, Koblenz, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Feiler
authors.forename: Monika
authors.initials: M
authors.lastname: Ratte
authors.forename: Gertie
authors.initials: G
authors.lastname: Arts
authors.forename: Christine
authors.initials: C
authors.lastname: Bazin
authors.forename: Frank
authors.initials: F
authors.lastname: Brauer
authors.forename: Carmen
authors.initials: C
authors.lastname: Casado
authors.forename: Laszlo
authors.initials: L
authors.lastname: Dören
authors.forename: Britta
authors.initials: B
authors.lastname: Eklund
authors.forename: Daniel
authors.initials: D
authors.lastname: Gilberg
authors.forename: Matthias
authors.initials: M
authors.lastname: Grote
authors.forename: Guido
authors.initials: G
authors.lastname: Gonsior
authors.forename: Christoph
authors.initials: C
authors.lastname: Hafner
authors.forename: Willi
authors.initials: W
authors.lastname: Kopf
authors.forename: Bernd
authors.initials: B
authors.lastname: Lemnitzer
authors.forename: Anja
authors.initials: A
authors.lastname: Liedtke
authors.forename: Uwe
authors.initials: U
authors.lastname: Matthias
authors.forename: Ewa
authors.initials: E
authors.lastname: Okos
authors.forename: Pascal
authors.initials: P
authors.lastname: Pandard
authors.forename: Dirk
authors.initials: D
authors.lastname: Scheerbaum
authors.forename: Mechthild
authors.initials: M
authors.lastname: Schmitt-Jansen
authors.forename: Kathleen
authors.initials: K
authors.lastname: Stewart
authors.forename: Ivana
authors.initials: I
authors.lastname: Teodorovic
authors.forename: Andrea
authors.initials: A
authors.lastname: Wenzel
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Pluta
mesh_terms: D059001:Aquatic Organisms; D002733:Chlorophenols / Q000633:toxicity; D019015:Geologic Sediments / Q000032:analysis*; D019684:Magnoliopsida / Q000187:drug effects* / Q000254:growth & development; D012015:Reference Standards; D015203:Reproducibility of Results; D018675:Toxicity Tests / Q000379:methods* / Q000592:standards; D014874:Water Pollutants, Chemical / Q000592:standards / Q000633:toxicity*
chemical_list: D002733:Chlorophenols; D014874:Water Pollutants, Chemical; C116190:3,5-dichlorophenol
keywords: Inter-laboratory trial; Macrophytes; Myriophyllum aquaticum; Sediment contact test; Sediment toxicity