title: Molecular characterization and expression analysis of Triticum aestivum squamosa-promoter binding protein-box genes involved in ear development.
abstract: Wheat (Triticum aestivum L.) is one of the most important crops in the world. Squamosa-promoter binding protein (SBP)-box genes play a critical role in regulating flower and fruit development. In this study, 10 novel SBP-box genes (TaSPL genes) were isolated from wheat ((Triticum aestivum L.) cultivar Yanzhan 4110). Phylogenetic analysis classified the TaSPL genes into five groups (G1-G5). The motif combinations and expression patterns of the TaSPL genes varied among the five groups with each having own distinctive characteristics: TaSPL20/21 in G1 and TaSPL17 in G2 mainly expressed in the shoot apical meristem and the young ear, and their expression levels responded to development of the ear; TaSPL6/15 belonging to G3 were upregulated and TaSPL1/23 in G4 were downregulated during grain development; the gene in G5 (TaSPL3) expressed constitutively. Thus, the consistency of the phylogenetic analysis, motif compositions, and expression patterns of the TaSPL genes revealed specific gene structures and functions. On the other hand, the diverse gene structures and different expression patterns suggested that wheat SBP-box genes have a wide range of functions. The results also suggest a potential role for wheat SBP-box genes in ear development. This study provides a significant beginning of functional analysis of SBP-box genes in wheat.
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, the Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.forename: Guangyao
authors.initials: G
authors.lastname: Zhao
authors.forename: Xinguo
authors.initials: X
authors.lastname: Mao
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.forename: Ruilian
authors.initials: R
authors.lastname: Jing
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D038081:Organogenesis / Q000235:genetics*; D012275:Oryza / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding / Q000235:genetics; D017434:Protein Structure, Tertiary; D060888:Real-Time Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000378:metabolism; D014908:Triticum / Q000033:anatomy & histology* / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D049452:Green Fluorescent Proteins
keywords: Expression profile; Triticum aestivum; grain yield; squamosa-promoter binding protein-box genes