title: Lectin coated MgO nanoparticle: its toxicity, antileishmanial activity, and macrophage activation.
abstract: The purpose of this research was to evaluate toxicity of uncoated magnesium oxide nanoparticles (MgO NPs), MgO NPs coated with Peanut agglutinin (PNA) lectin, and PNA alone on the promastigotes of Leishmania major (L. major) and macrophages of BALB/c mice. On the other hand, antileishmanial property of uncoated MgO NPs, lectin coated MgO NPs, and PNA lectin alone was evaluated, and also macrophage activation was investigated after treatment with these materials by measurement of nitrite, H2O2, and some interleukins. This study showed that PNA lectin and lectin coated MgO NPs had approximately no toxicity on L. major and macrophages, but some toxic effects were observed for uncoated MgO NPs, especially at concentration of 500 µg/mL. Interestingly, lectin coated MgO NPs had the highest antileishmanial activity and macrophage activation, compared with uncoated MgO NPs and PNA lectin.
authors.affiliation: Department of Medical Physics and Biomedical Engineering, Shahid Beheshti University of Medical Sciences , Tehran , Iran .
authors.forename: Ali
authors.initials: A
authors.lastname: Jebali
authors.forename: Seyedhossein
authors.initials: S
authors.lastname: Hekmatimoghaddam
authors.forename: Bahram
authors.initials: B
authors.lastname: Kazemi
authors.forename: Azra
authors.initials: A
authors.lastname: Allaveisie
authors.forename: Alireza
authors.initials: A
authors.lastname: Masoudi
authors.forename: Karim
authors.initials: K
authors.lastname: Daliri
authors.forename: Najme
authors.initials: N
authors.lastname: Sedighi
authors.forename: Javad
authors.initials: J
authors.lastname: Ranjbari
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D006861:Hydrogen Peroxide / Q000378:metabolism; D007378:Interleukins / Q000378:metabolism; D018320:Leishmania major / Q000187:drug effects; D008262:Macrophage Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism; D008277:Magnesium Oxide / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles; D009573:Nitrites / Q000378:metabolism; D019887:Peanut Agglutinin / Q000737:chemistry*
chemical_list: D000981:Antiprotozoal Agents; D007378:Interleukins; D009573:Nitrites; D019887:Peanut Agglutinin; D008277:Magnesium Oxide; D006861:Hydrogen Peroxide
keywords: Antileishmanial activity; MgO nanoparticle; lectin; toxicity