title: Protective activity of a novel resveratrol analogue, HS-1793, against DNA damage in 137Cs-irradiated CHO-K1 cells.
abstract: Resveratrol has received considerable attention as a polyphenol with anti-oxidant, anti-carcinogenic, and anti-inflammatory effects. Radiation is an important component of therapy for a wide range of malignant conditions. However, it causes damage to normal cells and, hence, can result in adverse side effects. This study was conducted to examine whether HS-1793, a novel resveratrol analogue free from the restriction of metabolic instability and the high dose requirement of resveratrol, induces a protective effect against radiation-induced DNA damage. HS-1793 effectively scavenged free radicals and inhibited radiation-induced plasmid DNA strand breaks in an in vitro assay. HS-1793 significantly decreased reactive oxygen species and cellular DNA damage in 2 Gy-irradiated Chinese hamster ovary (CHO)-K1 cells. In addition, HS-1793 dose-dependently reduced the levels of phosphorylated H2AX in irradiated CHO-K1 cells. These results indicate that HS-1793 has chemical radioprotective activity. Glutathione levels and superoxide dismutase activity in irradiated CHO-K1 cells increased significantly following HS-1793 treatment. The enhanced biological anti-oxidant activity and chemical radioprotective activity of HS-1793 maintained survival of irradiated CHO-K1 cells in a clonogenic assay. Therefore, HS-1793 may be of value as a radioprotector to protect healthy tissue surrounding tumor cells during radiotherapy to obtain better tumor control with a higher dose.
authors.affiliation: Department of Microbiology, Dong-A University College of Medicine, Daeshingongwon-gil 32, Seo-gu, Busan 619-953, Republic of Korea.
authors.forename: Min Ho
authors.initials: MH
authors.lastname: Jeong
authors.forename: Kwang Mo
authors.initials: KM
authors.lastname: Yang
authors.forename: Dong Hyeok
authors.initials: DH
authors.lastname: Jeong
authors.forename: Chang Geun
authors.initials: CG
authors.lastname: Lee
authors.forename: Su Jung
authors.initials: SJ
authors.lastname: Oh
authors.forename: Soo Kyung
authors.initials: SK
authors.lastname: Jeong
authors.forename: Ki Won
authors.initials: KW
authors.lastname: Lee
authors.forename: Young Rae
authors.initials: YR
authors.lastname: Jo
authors.forename: Wol Soon
authors.initials: WS
authors.lastname: Jo
mesh_terms: D000818:Animals; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects*; D002588:Cesium Radioisotopes / Q000494:pharmacology*; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage / Q000187:drug effects* / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D009284:Naphthols / Q000008:administration & dosage*; D011829:Radiation Dosage; D011836:Radiation Tolerance / Q000187:drug effects / Q000502:physiology*; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D012118:Resorcinols / Q000008:administration & dosage*
chemical_list: C523393:4-(6-hydroxy-2-naphthyl)-1,3-benzenediol; D002588:Cesium Radioisotopes; D009284:Naphthols; D011837:Radiation-Protective Agents; D012118:Resorcinols
keywords: DNA strand break; HS-1793; free radical scavenging activity; radioprotection; reactive oxygen species (ROS); resveratrol analogue