title: Luteolin, a bioflavonoid, attenuates azoxymethane-induced effects on mitochondrial enzymes in BALB/c mice.
abstract: Colon cancer (CRC) is a serious health problem throughout the world. Development of novel drugs without side effects for this cancer is crucial. Luteolin (LUT), a bioflavonoid, has many beneficial effects such as antioxidant, anti-inflammatory and anti-proliferative potential. was a potent chemical carcinogen used for the induction of colon cancer. Colon carcinogenesis was initiated by intraperitoneal injection of azoxymethane (AOM) to mice at the dose of 15 mg/body kg weight in Balb/C mice for 3 weeks. Mice were treated with LUT at the dose of 1.2 mg /body kg weight orally. Mitochondrial enzymes such as isocitrate dehydrogenase (ICDH), α-keto dehydrogenase (α-KDH), succinate dehydrogenase (SDH) and the activities of respiratory chain enzymes NADH dehydrogenase and cytochrome c oxidase were found to be elevated in AOM-treated animals. Treatment with LUT decreased the activities of all the parameters significantly. Hence, LUT might be a potent anticancer agent against colorectal cancer.
authors.affiliation: Department of Biochemistry, University of Madras, Guindy Campus, Chennai, India E-mail : sudhandiran@yahoo.com.
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Pandurangan
authors.forename: Suresh Kumar
authors.initials: SK
authors.lastname: Ananda Sadagopan
authors.forename: Prakash
authors.initials: P
authors.lastname: Dharmalingam
authors.forename: Sudhandiran
authors.initials: S
authors.lastname: Ganapasam
mesh_terms: D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide) / Q000378:metabolism; D000818:Animals; D001397:Azoxymethane / Q000633:toxicity*; D015153:Blotting, Western; D002273:Carcinogens / Q000633:toxicity*; D003106:Colon / Q000187:drug effects / Q000201:enzymology*; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000201:enzymology*; D042967:Electron Transport Complex I / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D047311:Luteolin / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008928:Mitochondria / Q000201:enzymology*; D013385:Succinate Dehydrogenase / Q000378:metabolism
chemical_list: D002273:Carcinogens; D007521:Isocitrate Dehydrogenase; D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV; D042967:Electron Transport Complex I; D047311:Luteolin; D001397:Azoxymethane