title: Sequence variability in four mitochondrial genes among pinworm Aspicularis tetraptera isolates from laboratory mice in four provinces, China.
abstract: This research aimed at exploring sequence variability in four mitochondrial (mt) genes, namely, cytochrome c oxidase subunit 1 (cox1), cytochrome b (cytb) and NADH dehydrogenase subunits 1 and 5 (nad1 and nad5), among pinworm Aspicularis tetraptera isolates from laboratory mice in four different provinces, China. A part of the cox1 (pcox1), cytb (pcytb), nad1 and nad5 genes (pnad1 and pnad5) were amplified separately from individual pinworms by polymerase chain reaction (PCR) and sequenced to determine sequence variations and examine their phylogenetic relationships. Herein, the intra-specific sequence variations within A. tetraptera were 0-0.5% for pcox1, 0-1.4% for pcytb, 0-1.8% for pnad1 and 0-1.7% for pnad5, respectively. In contrast, the inter-specific sequence differences among members of the Oxyuridae were significantly higher, being 13.7-17.0% for pcox1, 24.5-34.7% for pcytb, 26.6-29.6% for pnad1 and 24.4-25.5% for pnad5, respectively. Three methods, namely, Bayesian inference (BI), maximum likelihood (ML) and maximum parsimony (MP), were used for phylogenetic analyses based on the combined sequences of the four mt gene sequences, and the results indicated that all A. tetraptera samples form monophyletic groups, but samples from the same geographical origin did not always cluster together. This study demonstrated the existence of low-level intra-specific variation in four mtDNA sequences among A. tetraptera isolates from laboratory mice in different geographic regions in China, indicating no obvious geographical distinction among A. tetraptera isolates in China. These findings have important implications for studying systematics, molecular epidemiology and population genetics of A. tetraptera.
authors.affiliation: College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University , Xinfeng Road, Daqing, Heilongjiang Province , People's Republic of China .
authors.forename: Yan
authors.initials: Y
authors.lastname: Lou
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Qiu
authors.forename: Jun-Feng
authors.initials: JF
authors.lastname: Gao
authors.forename: Wen-Tao
authors.initials: WT
authors.lastname: Wang
authors.forename: Jing-Ying
authors.initials: JY
authors.lastname: Xiao
authors.forename: Qiao-Cheng
authors.initials: QC
authors.lastname: Chang
authors.forename: Chun-Ren
authors.initials: CR
authors.lastname: Wang
mesh_terms: D000818:Animals; D001499:Bayes Theorem; D002681:China; D045303:Cytochromes b / Q000235:genetics; D018509:DNA, Helminth / Q000302:isolation & purification; D004272:DNA, Mitochondrial / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000235:genetics; D004757:Enterobius / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D051379:Mice; D009245:NADH Dehydrogenase / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D045303:Cytochromes b; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV
keywords: Aspicularis tetraptera; laboratory mice; mitochondrial DNA; phylogenetic analyses; sequence variability