title: Pressurized water extraction of β-glucan enriched fractions with bile acids-binding capacities obtained from edible mushrooms.
abstract: A pressurized water extraction (PWE) method was developed in order to extract β-glucans with bile acids-binding capacities from cultivated mushrooms (Agaricus bisporus, Lentinula edodes, and Pleurotus ostreatus) to be used as supplements to design novel foods with hypocholesterolemic properties. Extraction yields were higher in individual than sequential extractions being the optimal extraction parameters: 200°C, 5 cycles of 5 min each at 10.3 MPa. The crude polysaccharide (PSC) fractions, isolated from the PWE extracts contained mainly β-glucans (including chitooligosaccharides deriving from chitin hydrolysis), α-glucans, and other PSCs (hetero-/proteo-glucans) depending on the extraction temperature and mushroom strain considered. The observed bile acids-binding capacities of some extracts were similar to a β-glucan enriched fraction obtained from cereals.
authors.affiliation: Dept. of Production and Characterization of Novel Foods, CIAL-Research Inst. in Food Science (UAM+CSIC), C/Nicolas Cabrera 9, Campus de Cantoblanco, Universidad Autónoma de Madrid, 28049, Madrid, Spain.
authors.forename: Marimuthu
authors.initials: M
authors.lastname: Palanisamy
authors.forename: Laila
authors.initials: L
authors.lastname: Aldars-García
authors.forename: Alicia
authors.initials: A
authors.lastname: Gil-Ramírez
authors.forename: Alejandro
authors.initials: A
authors.lastname: Ruiz-Rodríguez
authors.forename: Francisco R
authors.initials: FR
authors.lastname: Marín
authors.forename: Guillermo
authors.initials: G
authors.lastname: Reglero
authors.forename: Cristina
authors.initials: C
authors.lastname: Soler-Rivas
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000704:Analysis of Variance; D001647:Bile Acids and Salts / Q000378:metabolism*; D005591:Chemical Fractionation / Q000379:methods*; D062610:Fungal Polysaccharides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D005936:Glucans / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D011312:Pressure; D012997:Solvents; D014867:Water
chemical_list: D001647:Bile Acids and Salts; D062610:Fungal Polysaccharides; D005936:Glucans; D012997:Solvents; D014867:Water
keywords: accelerated solvent extraction; bile acids; cholesterol; oyster mushroom; shiitake mushroom; white button mushroom; β-glucans