title: Impact of long term Fe³⁺ toxicity on expression of glutathione system in rat liver.
abstract: The free radicals within the body, produced by metabolic activities or derived from environmental sources are relatively related to hepatoxicity. Since heavy metals including iron have the ability to produce free radicals, the liver glutathione system neutralizes them to protect cells against any damage. The objective of this study is to indicate the toxic effects of iron on the glutathione system at the enzymatic and molecular level. Thus, any possible correlation between enzymatic and molecular levels can be determined. According to our results, while mRNA expression of glutathione reductase (Gsr) and glutathione S-transferases (Gsta5) genes were not affected by long-term exposure to various concentrations of iron (Fe(3+)), transcription level of glutathione peroxidase (Gpx2) was influenced in the presence of toxic iron. Whereas the enzyme activites of GSR (GR), GPX and GST were significantly affected in rat liver.
authors.affiliation: Ataturk University, Science Faculty, Department of Molecular Biology and Genetics, 25240 Erzurum, Turkey. Electronic address: hbudak@atauni.edu.tr.
authors.forename: Harun
authors.initials: H
authors.lastname: Budak
authors.affiliation: Ataturk University, Science Faculty, Department of Molecular Biology and Genetics, 25240 Erzurum, Turkey.
authors.forename: Nurdan
authors.initials: N
authors.lastname: Gonul
authors.affiliation: Ataturk University, Science Faculty, Department of Molecular Biology and Genetics, 25240 Erzurum, Turkey.
authors.forename: Hamid
authors.initials: H
authors.lastname: Ceylan
authors.affiliation: Ataturk University, Science Faculty, Department of Chemistry, 25240 Erzurum, Turkey.
authors.forename: Enver Fehim
authors.initials: EF
authors.lastname: Kocpinar
mesh_terms: D000818:Animals; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism*; D005980:Glutathione Reductase / Q000235:genetics / Q000378:metabolism*; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism*; D007501:Iron / Q000097:blood / Q000493:pharmacokinetics / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007501:Iron; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione
keywords: Enzyme activity; Glutathione system; Iron; Toxic effects; mRNA expression