title: Effects of genistein and daidzein on cell proliferation kinetics in HT29 colon cancer cells: the expression of CTNNBIP1 (β-catenin), APC (adenomatous polyposis coli) and BIRC5 (survivin).
abstract: Soybean isoflavonoids have received significant attention due to their potential anticarcinogenic and antiproliferative effects and possible role in many signal transduction pathways. However, their mechanisms of action and their molecular targets remain to be further elucidated. In this paper, we demonstrated that two soybean isoflavones (genistein and daidzein) reduced the proliferation of the human colon adenocarcinoma grade II cell line (HT-29) at concentrations of 25 and 50-100 μM, respectively. We then investigated the effects of genistein and daidzein by RT-PCR on molecules that involved in tumor development and progression by their regulation of cell proliferation. At a concentration of 50 μM genistein, there was suppressed expression of β-catenin (CTNNBIP1). Neither genistein nor daidzein affected APC (adenomatous polyposis coli) or survivin (BIRC5) expression when cells were treated with concentrations of 10 or 50 μM. These data suggest that the down-regulation of β-catenin by genistein may constitute an important determinant of the suppression of HT-29 cell growth and may be exploited for the prevention and treatment of colon cancer.
authors.affiliation: General Biology Department, State University of Londrina (UEL), Rodovia Celso Garcia Cid, Pr 445 km 380, Campus Universitário, Cx. Postal 6001, CEP 86051-980, Londrina, PR, Brazil, lepri@uel.br.
authors.forename: Sandra Regina
authors.initials: SR
authors.lastname: Lepri
authors.forename: Leonardo Campos
authors.initials: LC
authors.lastname: Zanelatto
authors.forename: Patrícia Benites Gonçalves
authors.initials: PB
authors.lastname: da Silva
authors.forename: Daniele
authors.initials: D
authors.lastname: Sartori
authors.forename: Lucia Regina
authors.initials: LR
authors.lastname: Ribeiro
authors.forename: Mario Sergio
authors.initials: MS
authors.lastname: Mantovani
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology*; D025601:Adenomatous Polyposis Coli Protein / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D016588:Anticarcinogenic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D048708:Cell Growth Processes / Q000187:drug effects* / Q000235:genetics*; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology*; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects* / Q000235:genetics; D019833:Genistein / Q000494:pharmacology* / Q000627:therapeutic use; D019073:HT29 Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D007529:Isoflavones / Q000494:pharmacology* / Q000627:therapeutic use; D048789:Phytoestrogens / Q000494:pharmacology* / Q000627:therapeutic use; D008517:Phytotherapy; D013025:Glycine max; D000077022:Survivin
chemical_list: C555337:APC protein, human; D048868:Adaptor Proteins, Signal Transducing; D025601:Adenomatous Polyposis Coli Protein; D016588:Anticarcinogenic Agents; C107489:BIRC5 protein, human; C411982:CTNNBIP1 protein, human; D051034:Inhibitor of Apoptosis Proteins; D047908:Intracellular Signaling Peptides and Proteins; D007529:Isoflavones; D048789:Phytoestrogens; D000077022:Survivin; C004742:daidzein; D019833:Genistein