title: OsDREB2A, a rice transcription factor, significantly affects salt tolerance in transgenic soybean.
abstract: The dehydration responsive element binding (DREB) transcription factors play an important role in regulating stress-related genes. OsDREB2A, a member of the DREBP subfamily of AP2/ERF transcription factors in rice (Oryza sativa), is involved in the abiotic stress response. OsDREB2A expression is induced by drought, low-temperature and salt stresses. Here, we report the ability of OsDREB2A to regulate high-salt response in transgenic soybean. Overexpressing OsDREB2A in soybeans enhanced salt tolerance by accumulating osmolytes, such as soluble sugars and free proline, and improving the expression levels of some stress-responsive transcription factors and key genes. The phenotypic characterization of transgenic soybean were significantly better than those of wild-type (WT). Electrophoresis mobility shift assay (EMSA) revealed that the OsDREB2A can bind to the DRE core element in vitro. These results indicate that OsDREB2A may participate in abiotic stress by directly binding with DRE element to regulate the expression of downstream genes. Overexpression of OsDREB2A in soybean might be used to improve tolerance to salt stress.
authors.affiliation: The Guangdong Subcenter of National Center for Soybean Improvement, State Key Laboratory of Agricultural and Biological Resources Protection and Utilization in Subtropics, College of Agriculture, South China Agricultural University, Guangzhou, China.
authors.forename: Xiu-Xiang
authors.initials: XX
authors.lastname: Zhang
authors.affiliation: The Guangdong Subcenter of National Center for Soybean Improvement, State Key Laboratory of Agricultural and Biological Resources Protection and Utilization in Subtropics, College of Agriculture, South China Agricultural University, Guangzhou, China.
authors.forename: Yu-Juan
authors.initials: YJ
authors.lastname: Tang
authors.affiliation: The Guangdong Subcenter of National Center for Soybean Improvement, State Key Laboratory of Agricultural and Biological Resources Protection and Utilization in Subtropics, College of Agriculture, South China Agricultural University, Guangzhou, China.
authors.forename: Qi-Bin
authors.initials: QB
authors.lastname: Ma
authors.affiliation: The Guangdong Subcenter of National Center for Soybean Improvement, State Key Laboratory of Agricultural and Biological Resources Protection and Utilization in Subtropics, College of Agriculture, South China Agricultural University, Guangzhou, China.
authors.forename: Cun-Yi
authors.initials: CY
authors.lastname: Yang
authors.affiliation: The Guangdong Subcenter of National Center for Soybean Improvement, State Key Laboratory of Agricultural and Biological Resources Protection and Utilization in Subtropics, College of Agriculture, South China Agricultural University, Guangzhou, China.
authors.forename: Ying-Hui
authors.initials: YH
authors.lastname: Mu
authors.affiliation: The Guangdong Subcenter of National Center for Soybean Improvement, State Key Laboratory of Agricultural and Biological Resources Protection and Utilization in Subtropics, College of Agriculture, South China Agricultural University, Guangzhou, China.
authors.forename: Hai-Cui
authors.initials: HC
authors.lastname: Suo
authors.affiliation: School of Life Science, Jiaying University, Meizhou, China.
authors.forename: Lai-Hui
authors.initials: LH
authors.lastname: Luo
authors.affiliation: The Guangdong Subcenter of National Center for Soybean Improvement, State Key Laboratory of Agricultural and Biological Resources Protection and Utilization in Subtropics, College of Agriculture, South China Agricultural University, Guangzhou, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Nian
mesh_terms: D003080:Cold Temperature; D003681:Dehydration; D024202:Electrophoretic Mobility Shift Assay; D018506:Gene Expression Regulation, Plant; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011485:Protein Binding; D020218:Response Elements; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology; D013025:Glycine max / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D012965:Sodium Chloride