title: SNAP-tag technology optimized for use in Entamoeba histolytica.
abstract: Entamoeba histolytica is a protozoan parasite responsible for invasive intestinal and extraintestinal amebiasis. The pathology of amebiasis is still poorly understood, which can be largely attributed to lack of molecular tools. Here we present the optimization of SNAP-tag technology via codon optimization specific for E. histolytica. The resultant SNAP protein is highly expressed in amebic trophozoites, and shows proper localization when tagged with an endoplasmic reticulum retention signal. We further demonstrate the capabilities of this system using super resolution microscopy, done for the first time in E. histolytica.
authors.affiliation: University of Vermont Cellular, Molecular, and Biomedical Sciences Program, Burlington, Vermont, United States of America ; University of Vermont Department of Medicine, Burlington, Vermont, United States of America.
authors.forename: Adam
authors.initials: A
authors.lastname: Sateriale
authors.affiliation: University of Vermont Cellular, Molecular, and Biomedical Sciences Program, Burlington, Vermont, United States of America ; University of Vermont Microbiology and Molecular Genetics, Burlington, Vermont, United States of America.
authors.forename: Nathan H
authors.initials: NH
authors.lastname: Roy
authors.affiliation: University of Vermont Cellular, Molecular, and Biomedical Sciences Program, Burlington, Vermont, United States of America ; University of Vermont Microbiology and Molecular Genetics, Burlington, Vermont, United States of America ; University of Vermont Department of Medicine, Burlington, Vermont, United States of America.
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Huston
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000254:growth & development / Q000378:metabolism*; D004749:Entamoebiasis / Q000175:diagnosis / Q000378:metabolism; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D057054:Molecular Imaging / Q000379:methods*; D015335:Molecular Probes
chemical_list: D008565:Membrane Proteins; D015335:Molecular Probes