title: High Throughput FISH Analysis: A New, Sensitive Option For Evaluation of Hematological Malignancies.
abstract: OBJECTIVE
  The aim of this study was to determine the efficiency of the high throughput FISH analysis (HTFA) method for detecting genetic alterations in hematological malignancies, which is a new bacterial artificial chromosome array-based approach.
  
  
  METHODS
  We performed a HTFA study of bone marrow aspiration and peripheral blood samples of 77 cases (n=19 myelodysplastic syndrome, n=17 acute lymphoblastic leukemia, n=9 chronic myeloid leukemia, n=32 acute myeloid leukemia) with hematological malignancies during the periods of initial diagnosis, treatment, and/or follow-up.
  
  
  RESULTS
  Both numerical and structural abnormalities were detected by HTFA. We observed aberrations in 88% of our acute lymphoblastic leukemia patients, 25% of acute myeloid leukemia patients, and 31% of myelodysplastic syndrome patients. In chronic myeloid leukemia cases, aberration was not detected by HTFA.
  
  
  CONCLUSIONS
  Our results showed that HTFA, combined with other methods, will gradually take a place in the routine diagnosis of hematologic malignancies.
  
  
  BACKGROUND
  None declared.
authors.affiliation: Kocaeli University Faculty of Medicine, Department of Medical Genetics, Kocaeli, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Savlı
authors.affiliation: Kocaeli University Faculty of Medicine, Department of Medical Genetics, Kocaeli, Turkey.
authors.forename: Nilüfer
authors.initials: N
authors.lastname: Uzülmez
authors.affiliation: Kocaeli University Faculty of Medicine, Department of Medical Genetics, Kocaeli, Turkey.
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Ilkay
authors.affiliation: Kocaeli University Faculty of Medicine, Department of Medical Genetics, Kocaeli, Turkey.
authors.forename: Duygu
authors.initials: D
authors.lastname: Yavuz
authors.affiliation: Kocaeli University Faculty of Medicine, Department of Medical Genetics, Kocaeli, Turkey.
authors.forename: Deniz
authors.initials: D
authors.lastname: Sünnetçi
authors.affiliation: Kocaeli University Faculty of Medicine, Department of Hematology, Kocaeli, Turkey.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Hacıhanifioğlu
authors.affiliation: Kocaeli University Faculty of Medicine, Department of Medical Genetics, Kocaeli, Turkey.
authors.forename: Naci
authors.initials: N
authors.lastname: Cine
keywords: Hematologic malignancies; Leukemia; Microarray; molecular genetics