title: Complement plays a minimal role in Sm-p80-mediated protection against Schistosoma mansoni.
abstract: Sm-p80, the large subunit of Schistosoma masoni calpain, is a leading antigen candidate for a schistosome vaccine. Prophylactic and antifecundity efficacy of Sm-p80 has been tested using a variety of vaccine approaches. However, the mechanism of Sm-p80-mediated killing is still unknown. In this study, potential role of complement in Sm-p80-mediated protection was studied using both in vitro (cobra venom factor inhibition) and in vivo using mice deficient in C3 (C3 -/-; B6.129S4-C3tm1Crr/J). In the absence of C3, Sm-p80-based vaccine was able to provide significant reduction in adult worm burden following challenge with schistosome cercariae in mice suggesting the effector functions of complement may be limited in this vaccine-induced protection.
authors.affiliation: Center for Tropical Medicine and Infectious Diseases; Texas Tech University Health Sciences Center; Lubbock, TX USA; Department of Immunology and Molecular Microbiology; Texas Tech University Health Sciences Center; Lubbock, TX USA.
authors.forename: Souvik
authors.initials: S
authors.lastname: Karmakar
authors.affiliation: Center for Tropical Medicine and Infectious Diseases; Texas Tech University Health Sciences Center; Lubbock, TX USA; Department of Immunology and Molecular Microbiology; Texas Tech University Health Sciences Center; Lubbock, TX USA.
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Natural Sciences; School of Arts & Sciences; Peru State College; Peru, NE USA.
authors.forename: Gul
authors.initials: G
authors.lastname: Ahmad
authors.affiliation: Center for Tropical Medicine and Infectious Diseases; Texas Tech University Health Sciences Center; Lubbock, TX USA; Department of Immunology and Molecular Microbiology; Texas Tech University Health Sciences Center; Lubbock, TX USA.
authors.forename: Mayeen U
authors.initials: MU
authors.lastname: Alam
authors.affiliation: Center for Tropical Medicine and Infectious Diseases; Texas Tech University Health Sciences Center; Lubbock, TX USA; Department of Immunology and Molecular Microbiology; Texas Tech University Health Sciences Center; Lubbock, TX USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Winn
authors.affiliation: Tulane National Primate Research Center; Covington, LA USA.
authors.forename: Workineh
authors.initials: W
authors.lastname: Torben
authors.affiliation: Center for Tropical Medicine and Infectious Diseases; Texas Tech University Health Sciences Center; Lubbock, TX USA; Department of Immunology and Molecular Microbiology; Texas Tech University Health Sciences Center; Lubbock, TX USA.
authors.forename: Loc
authors.initials: L
authors.lastname: Le
authors.affiliation: Center for Tropical Medicine and Infectious Diseases; Texas Tech University Health Sciences Center; Lubbock, TX USA; Department of Immunology and Molecular Microbiology; Texas Tech University Health Sciences Center; Lubbock, TX USA.
authors.forename: Kory A
authors.initials: KA
authors.lastname: Tillery
authors.affiliation: Center for Tropical Medicine and Infectious Diseases; Texas Tech University Health Sciences Center; Lubbock, TX USA; Department of Immunology and Molecular Microbiology; Texas Tech University Health Sciences Center; Lubbock, TX USA; Department of Pathology; Texas Tech University Health Sciences Center; Lubbock, TX USA.
authors.forename: Afzal A
authors.initials: AA
authors.lastname: Siddiqui
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D002154:Calpain / Q000276:immunology*; D003176:Complement C3 / Q000172:deficiency / Q000276:immunology*; D004195:Disease Models, Animal; D007114:Immunization / Q000379:methods; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D059208:Parasite Load; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology
chemical_list: D000947:Antigens, Helminth; D003176:Complement C3; D002154:Calpain
keywords: Schistosoma; Sm-p80; calpain; complement; schistosomiasis; vaccine