title: Historical perspective, development and applications of next-generation sequencing in plant virology.
abstract: Next-generation high throughput sequencing technologies became available at the onset of the 21st century. They provide a highly efficient, rapid, and low cost DNA sequencing platform beyond the reach of the standard and traditional DNA sequencing technologies developed in the late 1970s. They are continually improved to become faster, more efficient and cheaper. They have been used in many fields of biology since 2004. In 2009, next-generation sequencing (NGS) technologies began to be applied to several areas of plant virology including virus/viroid genome sequencing, discovery and detection, ecology and epidemiology, replication and transcription. Identification and characterization of known and unknown viruses and/or viroids in infected plants are currently among the most successful applications of these technologies. It is expected that NGS will play very significant roles in many research and non-research areas of plant virology.
authors.affiliation: Consiglio per la ricerca e la Sperimentazione in Agricoltura, Centro di Ricerca per la Patologia Vegetale, Via C. G. Bertero 22, Rome 00156, Italy. marina.barba@entecra.it.
authors.forename: Marina
authors.initials: M
authors.lastname: Barba
authors.affiliation: Consiglio per la ricerca e la Sperimentazione in Agricoltura, Centro di Ricerca per la Patologia Vegetale, Via C. G. Bertero 22, Rome 00156, Italy. hanokh.czosnek@mail.huji.ac.il.
authors.forename: Henryk
authors.initials: H
authors.lastname: Czosnek
authors.affiliation: Consiglio per la ricerca e la Sperimentazione in Agricoltura, Centro di Ricerca per la Patologia Vegetale, Via C. G. Bertero 22, Rome 00156, Italy. ahadidi@yahoo.com.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Hadidi
mesh_terms: D059014:High-Throughput Nucleotide Sequencing / Q000379:methods* / Q000639:trends; D049673:History, 20th Century; D049674:History, 21st Century; D061066:Plant Pathology / Q000266:history* / Q000639:trends; D010942:Plant Viruses / Q000235:genetics*; D010944:Plants / Q000821:virology*; D014773:Virology / Q000379:methods* / Q000639:trends