title: Process integration for simultaneous saccharification, fermentation, and recovery (SSFR): production of butanol from corn stover using Clostridium beijerinckii P260.
abstract: A simultaneous saccharification, fermentation, and recovery (SSFR) process was developed for the production of acetone-butanol-ethanol (AB or ABE), of which butanol is the main product, from corn stover employing Clostridium beijerinckii P260. Of the 86 g L(-1) corn stover provided, over 97% of the sugars were released during hydrolysis and these were fermented completely with an ABE productivity of 0.34 g L(-1)h(-1) and yield of 0.39. This productivity is higher than 0.31 g L(-1)h(-1) when using glucose as a substrate demonstrating that AB could be produced efficiently from lignocellulosic biomass. Acetic acid that was released from the biomass during pretreatment and hydrolysis was also used by the culture to produce AB. An average rate of generation of sugars during corn stover hydrolysis was 0.98 g L(-1)h(-1). In this system AB was recovered using vacuum, and as a result of this (simultaneous product recovery), 100% sugars were used by the culture.
authors.affiliation: United States Department of Agriculture (USDA), ARS, National Center for Agricultural Utilization Research (NCAUR), Bioenergy Research Unit, 1815 N University Street, Peoria, IL 61604, USA. Electronic address: Nasib.Qureshi@ars.usda.gov.
authors.forename: N
authors.initials: N
authors.lastname: Qureshi
authors.affiliation: University of Illinois, Department of Agricultural & Biological Engineering, 360G Agricultural Engineering Sciences Building, 1304 W. Pennsylvania Avenue, Urbana, IL 61801, USA.
authors.forename: V
authors.initials: V
authors.lastname: Singh
authors.affiliation: USDA, ARS, NCAUR, Renewable Products Technology Unit, 1815 N University Street, Peoria, IL 61604, USA.
authors.forename: S
authors.initials: S
authors.lastname: Liu
authors.affiliation: The Ohio State University, Department of Animal Sciences, Ohio State Agricultural Research & Development Center (OARDC), 305 Gerlaugh Hall, 1680 Madison Avenue, Wooster, OH 44691, USA.
authors.forename: T C
authors.initials: TC
authors.lastname: Ezeji
authors.affiliation: United States Department of Agriculture (USDA), ARS, National Center for Agricultural Utilization Research (NCAUR), Bioenergy Research Unit, 1815 N University Street, Peoria, IL 61604, USA.
authors.forename: B C
authors.initials: BC
authors.lastname: Saha
authors.affiliation: United States Department of Agriculture (USDA), ARS, National Center for Agricultural Utilization Research (NCAUR), Bioenergy Research Unit, 1815 N University Street, Peoria, IL 61604, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Cotta
mesh_terms: D000096:Acetone / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D000440:Butanols / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D046970:Clostridium beijerinckii / Q000378:metabolism*; D005285:Fermentation; D012996:Solutions; D014866:Waste Products; D014867:Water / Q000737:chemistry; D003313:Zea mays / Q000378:metabolism*
chemical_list: D000440:Butanols; D012996:Solutions; D014866:Waste Products; D014867:Water; D000096:Acetone
keywords: Butanol/AB; Clostridium beijerinckii P260; Corn stover; Process integration; SSFR