title: Identification of Th cell epitopes on clfA adhesin of Staphylococcus aureus and characterization of their role in immunity.
abstract: OBJECTIVE
  The ClfA adhesin of Staphylococcus aureus is an excellent vaccine candidate antigen. CD4 + T cells play central roles during immune responses, but their functional contributions to Staphylococcus aureus in fection have yet to be evaluated.
  
  
  METHODS
  By using the SYFPEITHI prediction algorithm, we identified and characterized four Th epitopes within the ClfA adhesin.
  
  
  RESULTS
  Peptide C335 was I-Ed restricted Th1 type epitopes; peptides C214, C286, and C436 were I-Ad restricted Th2 type epitope.
  
  
  CONCLUSIONS
  The identification of these epitopes is important to evaluate and optimize the vaccine-primed protection against Staphylococcus aureus infection.
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Li
authors.affiliation: Fourth Hospital of Jilin University, Changchun 130062, China.
authors.forename: Yagang
authors.initials: Y
authors.lastname: Li
authors.affiliation: First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Ye
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D003030:Coagulase / Q000235:genetics / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology*; D005260:Female; D006801:Humans; D007109:Immunity; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D013203:Staphylococcal Infections / Q000276:immunology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D018829:Adhesins, Bacterial; C515944:ClfA protein, Staphylococcus aureus; D003030:Coagulase; D018984:Epitopes, T-Lymphocyte