title: ESCRT-I mediates FLS2 endosomal sorting and plant immunity.
abstract: The plant immune receptor FLAGELLIN SENSING 2 (FLS2) is present at the plasma membrane and is internalized following activation of its ligand flagellin (flg22). We show that ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT (ESCRT)-I subunits play roles in FLS2 endocytosis in Arabidopsis. VPS37-1 co-localizes with FLS2 at endosomes and immunoprecipitates with the receptor upon flg22 elicitation. Vps37-1 mutants are reduced in flg22-induced FLS2 endosomes but not in endosomes labeled by Rab5 GTPases suggesting a defect in FLS2 trafficking rather than formation of endosomes. FLS2 localizes to the lumen of multivesicular bodies, but this is altered in vps37-1 mutants indicating compromised endosomal sorting of FLS2 by ESCRT-I loss-of-function. VPS37-1 and VPS28-2 are critical for immunity against bacterial infection through a role in stomatal closure. Our findings identify that VPS37-1, and likewise VPS28-2, regulate late FLS2 endosomal sorting and reveals that ESCRT-I is critical for flg22-activated stomatal defenses involved in plant immunity.
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.
authors.forename: Thomas
authors.initials: T
authors.lastname: Spallek
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.
authors.forename: Martina
authors.initials: M
authors.lastname: Beck
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.
authors.forename: Sara
authors.initials: S
authors.lastname: Ben Khaled
authors.affiliation: Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Salomon
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.
authors.forename: Gildas
authors.initials: G
authors.lastname: Bourdais
authors.affiliation: Botanical Institute, Biocenter Cologne, Cologne, Germany.
authors.forename: Swen
authors.initials: S
authors.lastname: Schellmann
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom ; Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne, Germany.
authors.forename: Silke
authors.initials: S
authors.lastname: Robatzek
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000276:immunology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism; D004705:Endocytosis / Q000235:genetics*; D056827:Endosomal Sorting Complexes Required for Transport / Q000235:genetics* / Q000378:metabolism; D011992:Endosomes / Q000235:genetics / Q000378:metabolism; D057865:Plant Immunity / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D020696:rab5 GTP-Binding Proteins / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D011494:Protein Kinases; C411853:FLS2 protein, Arabidopsis; D020696:rab5 GTP-Binding Proteins