title: Effect of platelet lysate on human cells involved in different phases of wound healing.
abstract: BACKGROUND
  Platelets are rich in mediators able to positively affect cell activity in wound healing. Aim of this study was to characterize the effect of different concentrations of human pooled allogeneic platelet lysate on human cells involved in the different phases of wound healing (inflammatory phase, angiogenesis, extracellular matrix secretion and epithelialization).
  
  
  RESULTS
  Platelet lysate effect was studied on endothelial cells, monocytes, fibroblasts and keratinocytes, in terms of viability and proliferation, migration, angiogenesis, tissue repair pathway activation (ERK1/2) and inflammatory response evaluation (NFκB). Results were compared both with basal medium and with a positive control containing serum and growth factors. Platelet lysate induced viability and proliferation at the highest concentrations tested (10% and 20% v/v). Whereas both platelet lysate concentrations increased cell migration, only 20% platelet lysate was able to significantly promote angiogenic activity (p<0.05 vs. control), comparably to the positive control. Both platelet lysate concentrations activated important inflammatory pathways such as ERK1/2 and NFκB with the same early kinetics, whereas the effect was different for later time-points.
  
  
  CONCLUSIONS
  These data suggest the possibility of using allogeneic platelet lysate as both an alternative to growth factors commonly used for cell culture and as a tool for clinical regenerative application for wound healing.
authors.forename: Maria Chiara
authors.initials: MC
authors.lastname: Barsotti
authors.affiliation: Department of Surgery, Medical, Molecular, and Critical Area Pathology, University of Pisa, Pisa, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Chiara Barsotti
authors.affiliation: Institute of Clinical Physiology, National Research Council, Massa, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Losi
authors.affiliation: Institute of Clinical Physiology, National Research Council, Massa, Italy.
authors.forename: Enrica
authors.initials: E
authors.lastname: Briganti
authors.affiliation: Institute of Clinical Physiology, National Research Council, Massa, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Sanguinetti
authors.affiliation: Kedrion SpA, Castelvecchio Pascoli, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Magera
authors.affiliation: Institute of Clinical Physiology, National Research Council, Massa, Italy.
authors.forename: Tamer
authors.initials: T
authors.lastname: Al Kayal
authors.affiliation: Department of Surgery, Medical, Molecular, and Critical Area Pathology, University of Pisa, Pisa, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Feriani
authors.affiliation: Department of Surgery, Medical, Molecular, and Critical Area Pathology, University of Pisa, Pisa, Italy.
authors.forename: Rossella
authors.initials: R
authors.lastname: Di Stefano
authors.affiliation: Institute of Clinical Physiology, National Research Council, Massa, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Soldani
mesh_terms: D001792:Blood Platelets / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D020935:MAP Kinase Signaling System; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D014945:Wound Healing
chemical_list: D016328:NF-kappa B; C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3