title: Genome- and proteome-wide screening strategies for antigen discovery and immunogen design.
abstract: Infectious diseases remain a leading global cause of morbidity and mortality and there is an urgent need for effective approaches to develop vaccines, especially against complex pathogens. The availability of comprehensive genomic, proteomic and transcriptomic datasets has shifted the paradigm of vaccine development from microbiological to sequence-based approaches. However, how to effectively translate raw data into candidate vaccines is not yet obvious. Herein, we review cutting-edge technologies and screening strategies to mine genomic sequence information for state-of-the-art rational vaccine design, and highlight recent trends. Interdisciplinary approaches which cross the traditional boundaries of genomics, molecular biology, cell biology, immunology and computer science, and which prioritise antigens according to clinically relevant criteria, offer potential solutions to the widespread threat that complex pathogens pose to public health.
authors.affiliation: Infectious Diseases Programme, Queensland Institute of Medical Research, Herston, QLD 4006, Australia; School of Medicine, University of Queensland, Herston, QLD 4006, Australia. Electronic address: Sophie.Schussek@qimr.edu.au.
authors.forename: Sophie
authors.initials: S
authors.lastname: Schussek
authors.affiliation: Infectious Diseases Programme, Queensland Institute of Medical Research, Herston, QLD 4006, Australia. Electronic address: Angela.Trieu@qimr.edu.au.
authors.forename: Angela
authors.initials: A
authors.lastname: Trieu
authors.affiliation: Infectious Diseases Programme, Queensland Institute of Medical Research, Herston, QLD 4006, Australia; School of Medicine, University of Queensland, Herston, QLD 4006, Australia. Electronic address: Denise.Doolan@qimr.edu.au.
authors.forename: Denise L
authors.initials: DL
authors.lastname: Doolan
mesh_terms: D000941:Antigens / Q000235:genetics / Q000276:immunology; D016678:Genome / Q000235:genetics / Q000276:immunology; D023281:Genomics; D020543:Proteome / Q000032:analysis / Q000276:immunology; D040901:Proteomics; D014612:Vaccines / Q000235:genetics / Q000276:immunology
chemical_list: D000941:Antigens; D020543:Proteome; D014612:Vaccines
keywords: Immune profiling; Immunomics; Protection; Reverse vaccinology; Vaccinomics