title: Exercise is a potent stimulus for enhancing circulating DNase activity.
abstract: OBJECTIVE
  To elucidate cell free DNA (cfDNA) clearance kinetics following an acute bout of high intensity exercise by measuring circulating DNase activity reduction (AR).
  
  
  METHODS
  Serum cfDNA concentration and DNase-AR were measured prior to and post (immediately post, 7 and 30 min post) an acute bout of rowing exercise until exhaustion.
  
  
  RESULTS
  Serum cfDNA concentration was significantly (P ≤ .001) elevated immediately post (2.5-fold) and 7 min post exercise (2.3-fold) with a return close to baseline at 30 min post exercise (1.5-fold). The rise in cfDNA was accompanied by a concomitant, significant (P ≤ .001) decrease in serum DNase-AR from 15.1% prior to exercise to 3.1% AR at cessation of the exercise test and 7 min post exercise (3.9% AR). DNase-AR returned close to baseline at 30 min post exercise (5.2% AR).
  
  
  CONCLUSIONS
  A single bout of high intensity exercise is a potent stimulus for enhancing circulating DNase activity in healthy people. Acute exercise may therefore be considered as a non-pharmacological stimulus to trigger DNase activity. This finding may be relevant for pathological conditions associated with increased cfDNA concentrations like cystic fibrosis, where pharmacological recombinant human DNase (rhDNase) treatment has been successfully used to improve patients' health and physical function.
authors.affiliation: Division of Sports and Rehabilitation Medicine, Ulm University Medical Center, Germany.
authors.forename: Martina
authors.initials: M
authors.lastname: Velders
authors.affiliation: Division of Sports and Rehabilitation Medicine, Ulm University Medical Center, Germany.
authors.forename: Gunnar
authors.initials: G
authors.lastname: Treff
authors.affiliation: Division of Sports and Rehabilitation Medicine, Ulm University Medical Center, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Machus
authors.affiliation: Department of Health Sciences and Sports Medicine, Semmelweis University, Budapest, Hungary.
authors.forename: Edit
authors.initials: E
authors.lastname: Bosnyák
authors.affiliation: Division of Sports and Rehabilitation Medicine, Ulm University Medical Center, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Steinacker
authors.affiliation: Division of Sports and Rehabilitation Medicine, Ulm University Medical Center, Germany. Electronic address: uwe.schumann@uniklinik-ulm.de.
authors.forename: Uwe
authors.initials: U
authors.lastname: Schumann
mesh_terms: D000293:Adolescent; D000886:Anthropometry; D002474:Cell-Free System; D003851:Deoxyribonucleases / Q000097:blood*; D015444:Exercise; D006339:Heart Rate; D006801:Humans; D007700:Kinetics; D019344:Lactic Acid / Q000097:blood; D008297:Male; D013997:Time Factors
chemical_list: D019344:Lactic Acid; D003851:Deoxyribonucleases
keywords: Cell free DNA; Cystic fibrosis; DNase activity; Rowing