title: [Toxin profiles in fish implicated in ciguatera fish poisoning in Amami and Kakeroma Islands, Kagoshima Prefecture, Japan].
abstract: Ciguatoxins (CTXs) responsible for ciguatera fish poisoning (CFP) in Amami Islands, Kagoshima, Japan in 2008 were determined by LC-MS/MS analysis. Ciguatoxin-1B (CTX1B), 54-deoxyCTX1B, and 52-epi-54-deoxyCTX1B were detected in Variola louti and Lutjanus monostigma. The toxin profile distinctly differed from that of a CFP-related fish from Miyazaki, which mainly contained ciguatoxin-3C type toxins. Toxin profiles were species-specific, as observed in fish from Okinawa. The LC-MS/MS and mouse bioassay (MBA) methods produced comparable data, though 54-deoxyCTX1B was not taken into consideration owing to the lack of toxicity data. To improve assessment, toxicity data for this compound are needed. A reef fish caught on the same occasion and judged nontoxic by MBA (<0.025 MU/g) was found to contain low levels of CTX, indicating a potential risk for CFP.
authors.affiliation: Department of Health Sciences, Faculty of Medicine, University of the Ryukyus.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yogi
authors.forename: Naomasa
authors.initials: N
authors.lastname: Oshiro
authors.forename: Seiko
authors.initials: S
authors.lastname: Matsuda
authors.forename: Satsuki
authors.initials: S
authors.lastname: Sakugawa
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Matsuo
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yasumoto
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D036841:Ciguatera Poisoning / Q000209:etiology*; D002922:Ciguatoxins / Q000032:analysis* / Q000737:chemistry / Q000633:toxicity*; D005396:Fish Products / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D007564:Japan; D051379:Mice; D008968:Molecular Conformation; D053719:Tandem Mass Spectrometry / Q000379:methods; D018675:Toxicity Tests / Q000379:methods
chemical_list: D002922:Ciguatoxins