title: Scaffold/matrix attachment regions from CHO cell chromosome enhanced the stable transfection efficiency and the expression of transgene in CHO cells.
abstract: Two scaffold/matrix attachment regions (S/MARs) from Chinese hamster ovary (CHO) cell chromosomes were identified computationally and cloned as the domain boundaries regions. As partition chromosomes in independently regulated units to increase the exogenous gene expression, these two S/MARs (MAR1 and MAR6) flanked expression vectors were respectively transfected into CHO cells. The results showed that MAR1 and MAR6 were much more potent to increase the stable transfection efficiency than the chicken lysozyme gene 5' S/MARs, which had been experimentally identified to increasing the expression of heterologous gene. Moreover, MAR6 is more active in mediating high and consistent gene expression in CHO cells than the chicken lysozyme gene 5' S/MARs.
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Chang
authors.forename: Ruijie
authors.initials: R
authors.lastname: Liu
authors.forename: Qingzhe
authors.initials: Q
authors.lastname: Jin
authors.forename: Yuanfa
authors.initials: Y
authors.lastname: Liu
authors.forename: Xingguo
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D016466:CHO Cells; D003198:Computer Simulation; D006224:Cricetinae; D003412:Cricetulus; D018628:Gene Dosage; D042822:Genomic Instability; D045170:Matrix Attachment Regions / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection / Q000379:methods*; D019076:Transgenes / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins
keywords: Chinese hamster ovary cells; high expression; integration efficiency; scaffold/matrix attachment region