title: Attachment of human endothelial cells to polyester vascular grafts: pre-coating with adhesive protein assemblies and resistance to short-term shear stress.
abstract: Cardiovascular prosthetic bypass grafts do not endothelialize spontaneously in humans, and so they pose a thrombotic risk. Seeding with cells improves their performance, particularly in small-caliber applications. Knitted tubular polyethylene-terephthalate (PET) vascular prostheses (6 mm) with commercial type I collagen (PET/Co) were modified in the lumen by the adsorption of laminin (LM), by coating with a fibrin network (Fb) or a combination of Fb and fibronectin (Fb/FN). Primary human saphenous vein endothelial cells were seeded (1.50 × 10(5)/cm2), cultured for 72 h and exposed to laminar shear stress 15 dyn/cm(2) for 40 and 120 min. The control static grafts were excluded from shearing. The cell adherence after 4 h on PET/Co, PET/Co +LM, PET/Co +Fb and PET/Co +Fb/FN was 22%, 30%, 19% and 27% of seeding, respectively. Compared to the static grafts, the cell density on PET/Co and PET/Co +LM dropped to 61% and 50%, respectively, after 120 min of flow. The cells on PET/Co +Fb and PET/Co +Fb/FN did not show any detachment during 2 h of shear stress. Pre-coating the clinically-used PET/Co vascular prosthesis with LM or Fb/FN adhesive protein assemblies promotes the adherence of endothelium. Cell retention under flow is improved particularly on fibrin-containing (Fb and Fb/FN) surfaces.
authors.affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology Academy of Sciences of the Czech Republic, Prague, Czech Republic. jarach@centrum.cz.
authors.forename: J
authors.initials: J
authors.lastname: Chlupáč
authors.forename: E
authors.initials: E
authors.lastname: Filová
authors.forename: T
authors.initials: T
authors.lastname: Riedel
authors.forename: M
authors.initials: M
authors.lastname: Houska
authors.forename: E
authors.initials: E
authors.lastname: Brynda
authors.forename: M
authors.initials: M
authors.lastname: Remy-Zolghadri
authors.forename: R
authors.initials: R
authors.lastname: Bareille
authors.forename: P
authors.initials: P
authors.lastname: Fernandez
authors.forename: R
authors.initials: R
authors.lastname: Daculsi
authors.forename: C
authors.initials: C
authors.lastname: Bourget
authors.forename: L
authors.initials: L
authors.lastname: Bordenave
authors.forename: L
authors.initials: L
authors.lastname: Bačáková
mesh_terms: D000818:Animals; D001807:Blood Vessel Prosthesis / Q000592:standards; D002417:Cattle; D024042:Collagen Type I / Q000008:administration & dosage*; D042783:Endothelial Cells / Q000502:physiology*; D006801:Humans; D011091:Polyesters / Q000592:standards; D012501:Saphenous Vein / Q000166:cytology / Q000502:physiology; D033081:Shear Strength / Q000502:physiology*; D013314:Stress, Mechanical; D013997:Time Factors
chemical_list: D024042:Collagen Type I; D011091:Polyesters