title: Molecular and biological characterization of a recombinant isolate of potato virus Y from Mexico.
abstract: An isolate of potato virus Y (PVY), PVY-M3, was subjected to biological characterization on potato indicators and to whole-genome sequencing. PVY-M3 induced a local and systemic hypersensitive resistance (HR) response in potato cultivar Maris Bard expressing the Nz gene while inducing no HR in potato cultivars Desiree and King Edward, carrying Ny and Nc genes, respectively. These HR responses, combined with a lack of vein necrosis in tobacco, clearly defined PVY-M3 as an isolate of the PVY(Z) strain. Recombination analysis demonstrated that PVY-M3 had a typical European PVY(NTN) genome with three recombinant junctions, and PVY(N) and PVY(O) were identified as parents.
authors.affiliation: Department of PSES, University of Idaho, PO Box 442339, Moscow, ID, 83844-2339, USA.
authors.forename: Arturo
authors.initials: A
authors.lastname: Quintero-Ferrer
authors.forename: Loreto
authors.initials: L
authors.lastname: Robles-Hernandez
authors.forename: Ana C
authors.initials: AC
authors.lastname: Gonzalez-Franco
authors.forename: Camille
authors.initials: C
authors.lastname: Kerlan
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Karasev
mesh_terms: D015967:Gene Expression Regulation, Viral; D014644:Genetic Variation; D008800:Mexico; D010935:Plant Diseases / Q000276:immunology / Q000821:virology*; D017800:Potyvirus / Q000235:genetics* / Q000302:isolation & purification*; D016865:Reassortant Viruses / Q000235:genetics* / Q000302:isolation & purification; D011198:Solanum tuberosum / Q000821:virology*