title: Spectrofluorimetric Determination of α-Tocopherol in Capsules and Human Plasma.
abstract: A simple, sensitive and rapid spectrofluorimetric method for determination of α-tocopherol in pharmaceutical capsule and human plasma was developed and validated. The native fluorescence of α-tocopherol was measured at 334 nm with excitation at 291 nm, after extraction of α-tocopherol from human plasma hexane:dichloromethane mixture. The calibration curves were linear (R≥0.9993) in the concentration range of 0.25-2.5 μg/ml of α-tocopherol in both standard solutions and plasma samples. The developed method was directly and easily applied for determination of α-tocopherol in the plasma of healthy volunteers and different type of bladder cancer and stomach cancer patients and also pharmaceutical capsule.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, Erzurum-25240, Turkey.
authors.forename: Fatma
authors.initials: F
authors.lastname: Demirkaya-Miloglu
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, Erzurum-25240, Turkey.
authors.forename: Y
authors.initials: Y
authors.lastname: Kadioglu
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, Erzurum-25240, Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Senol
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, Erzurum-25240, Turkey.
authors.forename: M E
authors.initials: ME
authors.lastname: Yaman
keywords: cancer; human plasma; pharmaceutical; spectrofluorimetric method; α-tocopherol