title: Identification of endogenously S-nitrosylated proteins in Arabidopsis plantlets: effect of cold stress on cysteine nitrosylation level.
abstract: S-nitrosylation is a nitric oxide (NO)-based post-translational modification regulating protein function and signalling. We used a combination between the biotin switch method and labelling with isotope-coded affinity tag to identify endogenously S-nitrosylated peptides in Arabidopsis thaliana proteins extracted from plantlets. The relative level of S-nitrosylation in the identified peptides was compared between unstressed and cold-stress seedlings. We thereby detected 62 endogenously nitrosylated peptides out of which 20 are over-nitrosylated following cold exposure. Taken together these data provide a new repertoire of endogenously S-nitrosylated proteins in Arabidopsis with cysteine S-nitrosylation site. Furthermore they highlight the quantitative modification of the S-nitrosylation status of specific cysteine following cold stress.
authors.affiliation: UPMC Univ Paris 06, UR 5, Laboratoire de Physiologie Cellulaire et Moléculaire des Plantes, F-75005 Paris, France; CNRS, EAC 7180, Laboratoire de Physiologie Cellulaire et Moléculaire des Plantes, F-75005 Paris, France. Electronic address: juliette.puyaubert@upmc.fr.
authors.forename: Juliette
authors.initials: J
authors.lastname: Puyaubert
authors.affiliation: INRA, UR1199, Laboratoire de Protéomique Fonctionnelle, 34060 Montpellier Cedex, France.
authors.forename: Abasse
authors.initials: A
authors.lastname: Fares
authors.affiliation: UPMC Univ Paris 06, UR 5, Laboratoire de Physiologie Cellulaire et Moléculaire des Plantes, F-75005 Paris, France; CNRS, EAC 7180, Laboratoire de Physiologie Cellulaire et Moléculaire des Plantes, F-75005 Paris, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Rézé
authors.affiliation: INRA, UR1199, Laboratoire de Protéomique Fonctionnelle, 34060 Montpellier Cedex, France.
authors.forename: Jean-Benoît
authors.initials: JB
authors.lastname: Peltier
authors.affiliation: UPMC Univ Paris 06, UR 5, Laboratoire de Physiologie Cellulaire et Moléculaire des Plantes, F-75005 Paris, France; CNRS, EAC 7180, Laboratoire de Physiologie Cellulaire et Moléculaire des Plantes, F-75005 Paris, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Baudouin
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D003080:Cold Temperature; D003545:Cysteine / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D026403:S-Nitrosothiols / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D029681:Arabidopsis Proteins; D026403:S-Nitrosothiols; D009569:Nitric Oxide; D003545:Cysteine
keywords: 2-(N-morpholino)ethanesulfonic acid; 4,5-diaminofluorescein diacetate; ACN; ARR; Arabidopsis thaliana; BCA; BS; Biotin Switch; C-repeat Binding Factors genes; CBF; Cold stress; Cys; DAF2-DA; EDTA; GSNO; HENS; HPDP; Hepes buffer; ICAT; MES; MMTS; MS/MS; N-(6-(biotinamido)hexyl)-3′-(2′-pyridyldithio)propionamide; NO; Nitric oxide; PSI; PSII; PTM; Photosystem I; Photosystem II; Proteomics; Q-TOF; RuBP; S-Nitrosylation; S-adenosylhomocysteine hydrolase.; S-nitrosoglutathione; S-nitrosothiols; S-nitrosylated cysteine; SAHH; SDS; SNO; SNO-Cys; TFA; acetonitrile; bicinchoninic acid; cysteine; ethylenediaminetetraacetic acid; isotope-coded affinity tag; methyl methanethiosulfonate; neocuproine, SDS; nitric oxide; post-translational modification; quadrupole time-of-flight; ribulose 1,5-bisphosphate; sodium dodecylsulfate; tandem MS; trifluoroacetic acid; type-A Arabidopsis response regulator genes