title: Epidemiological investigation of community-acquired Staphylococcus aureus infection.
abstract: The current study aimed to reveal the pathogenic and spreading mechanisms of community-acquired Staphylococcus aureus (CA-SA) by analyzing its prevalence, drug resistance, virulence, and pathogenic factors. Historical information regarding CA skin and soft tissue infections were collected and disease characteristics were analyzed. Isolated CA-SA strains were subjected to antibiotic sensitivity tests using the agar dilution method, Staphylococcus cassette chromosome mec (SCCmec) tests, multilocus sequence typing (MLST), and staphylococcal protein A (SPA) and toxin gene screening. A total of 55 skin and soft tissue infections were investigated, and 12 strains of SA bacteria were isolated, which were all CA methicillin-susceptible SA (CA-MSSA). The antibiotic sensitivity tests showed that CA-MSSA was susceptible to all antibacterials with the exception of high resistance to erythromycin, clindamycin, tetracycline, gentamicin, and levofloxacin (ranging from 8.3 to 50%). The toxin detection results showed that the Panton-Valentine leukocidin (PVL) positive rate in CA-MSSA was 33.3%, the enterotoxin positive rate was 25%, and the toxic shock syndrome toxin-1 (TSSL) positive rate was 8.3%. No Staphylococcus enterotoxin H or Staphylococcus exfoliati was detected. MLST and SPA typing showed that the clones of CA-MSSA included ST5-t002 (2 strains), ST22-t309 (2 strains), ST398-t034, ST15-t5864, ST7-t091, ST25-t078, ST30-t318, ST121-t1425, ST800-t1425, and ST630-t377. No methicillin-resistant SA (MRSA) strains were detected in this study. CA-MSSA strains have high drug susceptibility and diverse genetic backgrounds. The coexistence of multiple toxins may provide a survival advantage in community dissemination for CA-MSSA.
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of the General Hospital of the People's Liberation Army, Beijing, China.
authors.forename: W
authors.initials: W
authors.lastname: Jiang
authors.forename: Z
authors.initials: Z
authors.lastname: Zhou
authors.forename: K
authors.initials: K
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Yu
mesh_terms: D015373:Bacterial Typing Techniques; D017714:Community-Acquired Infections / Q000188:drug therapy / Q000453:epidemiology*; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000453:epidemiology*; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors