title: High-performance liquid chromatography purification of chemically modified RNA aptamers.
abstract: 2'-Fluoro modified RNAs are useful as potential therapeutics and as special substrates for studying RNA function. 2'-Fluoro modified RNAs generally need to be purified after they are prepared either enzymatically or by solid-phase synthesis. Here we introduce a protocol by which 2'-fluoro modified RNAs with 57 and 58 nucleotides can be resolved and purified using ion-pair, reverse-phase high-performance liquid chromatography (HPLC). Because the size of our RNA samples is in the range of many known RNA aptamers of therapeutic values, our protocol should be generally useful.
authors.affiliation: Department of Chemistry, Center for Neuroscience Research, University at Albany, State University of New York (SUNY), Albany, NY 12222, USA.
authors.forename: Chi-Yen
authors.initials: CY
authors.lastname: Lin
authors.affiliation: Department of Chemistry, Center for Neuroscience Research, University at Albany, State University of New York (SUNY), Albany, NY 12222, USA.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Chemistry, Center for Neuroscience Research, University at Albany, State University of New York (SUNY), Albany, NY 12222, USA.
authors.forename: William
authors.initials: W
authors.lastname: Jaremko
authors.affiliation: Department of Chemistry, Center for Neuroscience Research, University at Albany, State University of New York (SUNY), Albany, NY 12222, USA. Electronic address: lniu@albany.edu.
authors.forename: Li
authors.initials: L
authors.lastname: Niu
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D054879:Halogenation; D007477:Ions / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D007477:Ions
keywords: Chemically modified RNA; HPLC; RNA purification and separation