title: Protective effects of mangosteen extract on H2O2-induced cytotoxicity in SK-N-SH cells and scopolamine-induced memory impairment in mice.
abstract: Mangosteen extracts (ME) contain high levels of polyphenolic compounds and antioxidant activity. Protective effects of ME against β-amyloid peptide (Aβ), induced cytotoxicity have been reported. Here, we further studied the protective effects of ME against oxidative stress induced by hydrogen peroxide (H2O2) and polychlorinated biphenyls (PCBs), and demonstrated the protection against memory impairment in mice. The cytoprotective effects of ME were measured as cell viability and the reduction in ROS activity. In SK-N-SH cell cultures, 200 μg/ml ME could partially antagonize the effects of 150 or 300 µM H2O2 on cell viability, ROS level and caspase-3 activity. At 200, 400 or 800 µg/ml, ME reduced AChE activity of SK-N-SH cells to about 60% of the control. In vivo study, Morris water maze and passive avoidance tests were used to assess the memory of the animals. ME, especially at 100 mg/kg body weight, could improve the animal's memory and also antagonize the effect of scopolamine on memory. The increase in ROS level and caspase-3 activity in the brain of scopolamine-treated mice were antagonized by the ME treatment. The study demonstrated cytoprotective effects of ME against H2O2 and PCB-52 toxicity and having AChE inhibitory effect in cell culture. ME treatment in mice could attenuate scopolamine-induced memory deficit and oxidative stress in brain.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Jintana
authors.initials: J
authors.lastname: Sattayasai
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Pongsatorn
authors.initials: P
authors.lastname: Chaonapan
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Tarinee
authors.initials: T
authors.lastname: Arkaravichie
authors.affiliation: Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Rungtip
authors.initials: R
authors.lastname: Soi-Ampornkul
authors.affiliation: Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Sarawut
authors.initials: S
authors.lastname: Junnu
authors.affiliation: Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Patcharakajee
authors.initials: P
authors.lastname: Charoensilp
authors.affiliation: Department of Microbiology, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
authors.forename: Jutima
authors.initials: J
authors.lastname: Samer
authors.affiliation: Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Jiraporn
authors.initials: J
authors.lastname: Jantaravinid
authors.affiliation: Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Patarabutr
authors.initials: P
authors.lastname: Masaratana
authors.affiliation: Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Bhoom
authors.initials: B
authors.lastname: Suktitipat
authors.affiliation: Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Juthatip
authors.initials: J
authors.lastname: Manissorn
authors.affiliation: Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Visith
authors.initials: V
authors.lastname: Thongboonkerd
authors.affiliation: Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Neelobol
authors.initials: N
authors.lastname: Neungton
authors.affiliation: Department of Microbiology, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
authors.forename: Primchanien
authors.initials: P
authors.lastname: Moongkarndi
mesh_terms: D000818:Animals; D003603:Cytotoxins / Q000494:pharmacology*; D029762:Garcinia mangostana / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008297:Male; D008569:Memory Disorders / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control; D051379:Mice; D008813:Mice, Inbred ICR; D018727:Muscarinic Antagonists / Q000009:adverse effects* / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012601:Scopolamine / Q000009:adverse effects* / Q000494:pharmacology
chemical_list: D003603:Cytotoxins; D018727:Muscarinic Antagonists; D016877:Oxidants; D010936:Plant Extracts; D006861:Hydrogen Peroxide; D012601:Scopolamine