title: Prereplication-complex formation: a molecular double take?
abstract: In G1, two copies of the MCM2-7 helicase are recruited to each origin of replication. Whereas recruitment of the first MCM2-7 is likely to be analogous to the loading of sliding clamps around DNA, how the second MCM2-7 complex is recruited is highly contentious. Here, we argue that MCM2-7 loading involves specific modifications to the clamp-loading reaction and propose that the first and second MCM2-7 molecules are loaded via similar mechanisms.
authors.affiliation: 1] Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA. [2] Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, UK.
authors.forename: Hasan
authors.initials: H
authors.lastname: Yardimci
authors.affiliation: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Johannes C
authors.initials: JC
authors.lastname: Walter
mesh_terms: D004247:DNA / Q000737:chemistry*; D064110:Minichromosome Maintenance Proteins / Q000378:metabolism; D051738:Origin Recognition Complex
chemical_list: D051738:Origin Recognition Complex; D004247:DNA; D064110:Minichromosome Maintenance Proteins