title: Succinic acid production from sucrose by Actinobacillus succinogenes NJ113.
abstract: In this study, sucrose, a reproducible disaccharide extracted from plants, was used as the carbon source for the production of succinic acid by Actinobacillus succinogenes NJ113. During serum bottle fermentation, the succinic acid concentration reached 57.1g/L with a yield of 71.5%. Further analysis of the sucrose utilization pathways revealed that sucrose was transported and utilized via a sucrose phosphotransferase system, sucrose-6-phosphate hydrolase, and a fructose PTS. Compared to glucose utilization in single pathway, more pathways of A. succinogenes NJ113 are dependent on sucrose utilization. By changing the control strategy in a fed-batch culture to alleviate sucrose inhibition, 60.5g/L of succinic acid was accumulated with a yield of 82.9%, and the productivity increased by 35.2%, reaching 2.16g/L/h. Thus utilization of sucrose has considerable potential economics and environmental meaning.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Puzhu South Road 30#, Nanjing 211816, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Puzhu South Road 30#, Nanjing 211816, PR China.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Dai
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Puzhu South Road 30#, Nanjing 211816, PR China.
authors.forename: Yonglan
authors.initials: Y
authors.lastname: Xi
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Puzhu South Road 30#, Nanjing 211816, PR China.
authors.forename: Mingke
authors.initials: M
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Puzhu South Road 30#, Nanjing 211816, PR China.
authors.forename: Xiangping
authors.initials: X
authors.lastname: Kong
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Puzhu South Road 30#, Nanjing 211816, PR China. Electronic address: majiangfeng@njut.edu.cn.
authors.forename: Jiangfeng
authors.initials: J
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Puzhu South Road 30#, Nanjing 211816, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Puzhu South Road 30#, Nanjing 211816, PR China.
authors.forename: Kequan
authors.initials: K
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Puzhu South Road 30#, Nanjing 211816, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
mesh_terms: D000188:Actinobacillus / Q000187:drug effects / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D001692:Biological Transport / Q000187:drug effects; D002244:Carbon / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D005632:Fructose / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D010770:Phosphotransferases / Q000378:metabolism; D019802:Succinic Acid / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism*
chemical_list: D005632:Fructose; D013395:Sucrose; D002244:Carbon; D019802:Succinic Acid; D010770:Phosphotransferases
keywords: A. succinogenes NJ113; Fed-batch culture; Succinic acid; Sucrose; Utilization pathways