title: Licochalcone B inhibits growth of bladder cancer cells by arresting cell cycle progression and inducing apoptosis.
abstract: To examine the mechanisms by which licochalcone B (LCB) inhibits the proliferation of human malignant bladder cancer cell lines (T24 and EJ) in vitro and antitumor activity in vivo in MB49 (murine bladder cancer cell line) tumor model. Exposure of T24 or EJ cells to LCB significantly inhibited cell lines proliferation in a concentration-dependent and time-dependent manner, and resulted in S phase arrest in T24 or EJ cells, respectively. LCB treatment decreased the expression of cyclin A, cyclin-dependent kinase (CDK1 and CDK2) mRNA, cell division cycle 25 (Cdc25A and Cdc25B) protein. In addition, LCB treatment down-regulated Bcl-2 and survivin expression, enhanced Bax expression, activated caspase-3 and cleaved poly (ADP-ribose) polymerase (PARP) protein. Consistently, the tumorigenicity of LCB-treated MB49 cells was limited significantly by using the colony formation assay in vitro and the MB49 tumor model performed in C57BL/6 mice in vivo. These findings provide support for the use of LCB in chemoprevention and bladder cancer therapy.
authors.affiliation: Institute of Urology, Second Hospital, Lanzhou University, 730030 Lanzhou, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Institute of Urology, Second Hospital, Lanzhou University, 730030 Lanzhou, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Institute of Urology, Second Hospital, Lanzhou University, 730030 Lanzhou, China.
authors.forename: Erlong
authors.initials: E
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Xinjiang Endemic Phytomedicine Resources, Ministry of Education, School of Pharmacy, Shihezi University, 832002 Shihezi, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Institute of Urology, Second Hospital, Lanzhou University, 730030 Lanzhou, China.
authors.forename: Yongqian
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Urology, Second Hospital, Lanzhou University, 730030 Lanzhou, China.
authors.forename: Shengjun
authors.initials: S
authors.lastname: Fu
authors.affiliation: Institute of Modern Physics, Chinese Academy of Sciences, 730000 Lanzhou, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Gan
authors.affiliation: Life Science School, Yantai University, 264000 Yantai, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Xinjiang Endemic Phytomedicine Resources, Ministry of Education, School of Pharmacy, Shihezi University, 832002 Shihezi, China; Life Science School, Yantai University, 264000 Yantai, China. Electronic address: zqsyt@sohu.com.
authors.forename: Qiusheng
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: Institute of Urology, Second Hospital, Lanzhou University, 730030 Lanzhou, China. Electronic address: erywzp@lzu.edu.cn.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001483:Base Sequence; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects*; D018797:Cell Cycle Proteins / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D047188:Chalcones / Q000494:pharmacology*; D017931:DNA Primers; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D018797:Cell Cycle Proteins; D047188:Chalcones; D017931:DNA Primers; C541528:licochalcone B
keywords: Apoptosis; Bladder cancer; Licochalcone B; S phase arrest