title: Rosmarinic acid extract for antioxidant, antiallergic, and α-glucosidase inhibitory activities, isolated by supramolecular technique and solvent extraction from Perilla leaves.
abstract: Rosmarinic acid extract with potent biological activities was successfully isolated by supramolecular technique and solvent extraction from Perilla leaves. By the supramolecular complex which was formed from flavocommelin and Perilla leaf extract as initial materials, the supernatant containing rosmarinic acid was isolated. Rosmarinic acid extract (62.9 ± 4.5% purity) was partly purified by partitioning ethyl acetate and water. Rosmarinic acid extract exhibited high total phenolic content of 433.9 ± 58.6 μg/mg of gallic acid equivalent, effective DPPH radical scavenging activity (SC50 of 5.5 ± 0.2 μg/mL), antiallergic activity (IC50 of 52.9 ± 6.7 μg/mL), and α-glucosidase inhibitory activity (IC50 of 0.23 ± 0.01 mg/mL). Rosmarinic acid extract shows high potential for diabetes mellitus and allergy treatments by inhibiting α-glucosidase activity and measuring β-hexosaminidase, related to life-style disease.
authors.affiliation: The United Graduate School of Agricultural Sciences, Ehime University , 3-5-7 Tarumi, Matsuyama, 790-8566, Japan.
authors.forename: Fengxian
authors.initials: F
authors.lastname: Zhu
authors.forename: Takayuki
authors.initials: T
authors.lastname: Asada
authors.forename: Akihiko
authors.initials: A
authors.lastname: Sato
authors.forename: Yoriko
authors.initials: Y
authors.lastname: Koi
authors.forename: Hisashi
authors.initials: H
authors.lastname: Nishiwaki
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Tamura
mesh_terms: D018926:Anti-Allergic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002934:Cinnamates / Q000302:isolation & purification / Q000494:pharmacology*; D053630:Depsides / Q000302:isolation & purification / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D065089:Glycoside Hydrolase Inhibitors; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D031344:Perilla frutescens / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism; D000097123:Rosmarinic Acid
chemical_list: D018926:Anti-Allergic Agents; D000975:Antioxidants; D002934:Cinnamates; D053630:Depsides; D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D001619:beta-N-Acetylhexosaminidases; D000097123:Rosmarinic Acid