title: [Influence of gelatin-alginian matrixes on morphological and functional changes of blood leukocytes].
abstract: BACKGROUND
  Knowledge of the relation of biomaterials and living tissues constitutes necessary information which should be used when composing a set of optimal carriers, e.g. for drugs or preparations supporting blood clotting.
  
  
  OBJECTIVE
  This paper presents an assessment of the influence of contact of gelatin-alginian matrixes with blood on leukocyte reactivity: the ability of mononuclear cells (lymphocytes and monocytes) to create a radial segmentation of nuclei--RS (the morphological change), and the ability of leukocytes to phagocytosis of yeast Saccharomyces cerevisiae cells and to produce active oxygen derivatives (functional changes).
  
  
  METHODS
  After having contact with the matrixes, the test of induced and spontaneous RS, the phagocytic test and nitroblue tetrazolium reduction test for blood leukocytes were performed.
  
  
  RESULTS
  The obtained results showed a decrease in the ability of mononuclear cells to form RS and in the ability of granulocytes to reduce nitroblue tetrazolium--NBT, but an increase in their phagocytic activity.
  
  
  CONCLUSIONS
  Temporary contact of gelatin-alginian matrixes with blood did not cause any morphological changes in the leukocytes. However, changes of their reactivity were observed.
authors.affiliation: Katedra Immunologii, Patofizjologii i Prewencji Weterynaryjnej, Wydział Medycyny Weterynaryjnej, Uniwersytet Przyrodniczy we Wrocławiu, Wrocław, Polska. aleksandra.krol@up.wroc.pl
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Pliszczak-Król
authors.affiliation: Zakład Chirurgii Eksperymentalnej i Badania Biomateriałów, Uniwersytet Medyczny im. Piastów Slaskich we Wrocławiu, Wrocław, Polska.
authors.forename: Maria
authors.initials: M
authors.lastname: Szymonowicz
authors.affiliation: Katedra Patologii, Wydział Medycyny Weterynaryjnej, Uniwersytet Przyrodniczy we Wrocławiu, Wrocław, Polska.
authors.forename: Jarosław
authors.initials: J
authors.lastname: Król
authors.affiliation: Zakład Chirurgii Eksperymentalnej i Badania Biomateriałów, Uniwersytet Medyczny im. Piastów Slaskich we Wrocławiu, Wrocław, Polska.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Rybak
authors.affiliation: Katedra Immunologii, Patofizjologii i Prewencji Weterynaryjnej, Wydział Medycyny Weterynaryjnej, Uniwersytet Przyrodniczy we Wrocławiu, Wrocław, Polska.
authors.forename: Stanisław
authors.initials: S
authors.lastname: Graczyk
authors.affiliation: Katedra i Zakład Technologii Postaci Leku, Uniwersytet Medyczny im. Piastów Slaskich we Wrocławiu, Wrocław, Polska.
authors.forename: Dorota
authors.initials: D
authors.lastname: Haznar
authors.affiliation: Katedra i Zakład Technologii Postaci Leku, Uniwersytet Medyczny im. Piastów Slaskich we Wrocławiu, Wrocław, Polska.
authors.forename: Janusz
authors.initials: J
authors.lastname: Pluta
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D004337:Drug Carriers / Q000494:pharmacology*; D005781:Gelatin Sponge, Absorbable / Q000494:pharmacology*; D066298:In Vitro Techniques; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D008422:Materials Testing; D010587:Phagocytosis / Q000187:drug effects; D013552:Swine
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers