title: SmeOP-TolCSm efflux pump contributes to the multidrug resistance of Stenotrophomonas maltophilia.
abstract: A five-gene cluster, tolCSm-pcm-smeRo-smeO-smeP, of Stenotrophomonas maltophilia was characterized. The presence of smeOP and smeRo-pcm-tolCSm operons was verified by reverse transcription (RT)-PCR. Both operons were negatively regulated by the TetR-type transcriptional regulator SmeRo, as demonstrated by quantitative RT-PCR and a promoter-fusion assay. SmeO and SmeP were associated with TolCSm (the TolC protein of S. maltophilia) for the assembly of a resistance-nodulation-cell-division (RND)-type pump. The compounds extruded by SmeOP-TolCSm mainly included nalidixic acid, doxycycline, amikacin, gentamicin, erythromycin, leucomycin, carbonyl cyanide 3-chlorophenylhydrazone, crystal violet, sodium dodecyl sulfate, and tetrachlorosalicylanilide.
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan.
authors.forename: Cheng-Wen
authors.initials: CW
authors.lastname: Lin
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Huang
authors.forename: Rouh-Mei
authors.initials: RM
authors.lastname: Hu
authors.forename: Tsuey-Ching
authors.initials: TC
authors.lastname: Yang
mesh_terms: D000583:Amikacin / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004318:Doxycycline / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D005839:Gentamicins / Q000494:pharmacology; D026901:Membrane Transport Proteins; D008826:Microbial Sensitivity Tests; D009268:Nalidixic Acid / Q000494:pharmacology; D009876:Operon / Q000235:genetics / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020615:Stenotrophomonas maltophilia / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D005839:Gentamicins; D026901:Membrane Transport Proteins; D009268:Nalidixic Acid; D000583:Amikacin; D004318:Doxycycline