title: [Effect of shuangshen ningxin formula on energy metabolism of myocardial ischemia/reperfusion rats].
abstract: OBJECTIVE
  To investigate the effect of Shuangshen Ningxin (SSNX) formula on energy metabolism of myocardial ischemia/reperfusion rats.
  
  
  METHODS
  The myocardial ischemia/reperfusion model of Wistar rats was established through the ligation of left anterior descending branch of coronary artery of for 40 min and the reperfusion for 2 h. The Wistar rats were randomly divided into six groups: the sham operation group, the model group, the Trimetazidine group (10 mg x kg(-1)) and SSNX groups (22.5, 45, 90 mg x kg(-1)). Preventive administration was conducted for 5 d. The operation was performed at 1 h on the day of the last administration. CK-MB assay kit was adopted to detect the activity of serum CK-MB. HPLC was used to determine ATP, ADP and AMP contents in myocardial tissues and calculate TAN and EC.
  
  
  RESULTS
  The preventive administration with SSNX could reduce the activity of serum CK-MB and increase ATP content and EC level in myocardial tissues (P < 0.01 or P < 0.05 vs. the model group).
  
  
  CONCLUSIONS
  SSNX formula can maintain energy charge in cardiomyocytes and relieve ischemia/reperfusion injury by preserving ischemic myocardium ATP.
authors.affiliation: Experimental Research Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China. lixuli1801@163.com
authors.forename: Xue-Li
authors.initials: XL
authors.lastname: Li
authors.affiliation: Experimental Research Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China.
authors.forename: Jian-Xun
authors.initials: JX
authors.lastname: Liu
authors.affiliation: Experimental Research Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China.
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Guo
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000818:Animals; D052279:Creatine Kinase, MB Form / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D004734:Energy Metabolism / Q000187:drug effects*; D006801:Humans; D008297:Male; D017202:Myocardial Ischemia / Q000188:drug therapy* / Q000378:metabolism* / Q000601:surgery; D015425:Myocardial Reperfusion; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004365:Drugs, Chinese Herbal; C532374:shuangshen ningxin; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D052279:Creatine Kinase, MB Form