title: Phenotypic and functional characterization of Glioblastoma cancer stem cells identified through 5-aminolevulinic acid-assisted surgery [corrected].
abstract: 5-aminolevulinic acid (5-ALA) introduction in the surgical management of Glioblastoma (GBM) enables the intra-operatively identification of cancer cells in the mass by means of fluorescence. Here, we analyzed the phenotype of GBM cells isolated from distinct tumour areas determined by 5-ALA (tumour core, 5-ALA intense and vague layers) and the potency of 5-ALA labelling in identifying GBM cells and cancer stem cells (CSCs) in the mass. 5-ALA identified distinct layers in the mass, with less differentiated cells residing in the core of the tumour. 5-ALA was able to stain up to 68.5% of CD133(+) cells in the 5-ALA intense layer and, although 5-ALA(+) cells retrieved from different tumour areas contained a similar proportion of CD133(+) cells (range 27.5-35.6%), those from the vague layer displayed the lowest ability to self-renew. In conclusion, our data demonstrate that a substantial amount of GBM cells and CSCs in the mass are able to avoid 5-ALA labelling and support the presence of heterogenic CSC populations in the GBM mass.
authors.affiliation: Oncohematology Laboratory, Department of Woman and Child Health, University of Padova, Via Giustiniani 3, 35128, Padua, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Rampazzo
authors.forename: Alessandro
authors.initials: A
authors.lastname: Della Puppa
authors.forename: Chiara
authors.initials: C
authors.lastname: Frasson
authors.forename: Giusy
authors.initials: G
authors.lastname: Battilana
authors.forename: Sara
authors.initials: S
authors.lastname: Bianco
authors.forename: Renato
authors.initials: R
authors.lastname: Scienza
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Basso
authors.forename: Luca
authors.initials: L
authors.lastname: Persano
mesh_terms: D000071916:AC133 Antigen; D000622:Aminolevulinic Acid / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D001706:Biopsy; D001932:Brain Neoplasms / Q000473:pathology* / Q000601:surgery; D005434:Flow Cytometry; D005909:Glioblastoma / Q000473:pathology* / Q000601:surgery; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D017319:Photosensitizing Agents / Q000378:metabolism
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D019394:Ki-67 Antigen; D009419:Nerve Tissue Proteins; C000606023:PROM1 protein, human; D010455:Peptides; D017319:Photosensitizing Agents; D000622:Aminolevulinic Acid