title: Mutations in the D-channel of cytochrome c oxidase causes leakage of the proton pump.
abstract: It has experimentally been found that certain mutations close to the entry point of the proton transfer channel in cytochrome c oxidase stop proton translocation but not the oxygen reduction chemistry. This effect is termed uncoupling. Since the mutations are 20Å away from the catalytic center, this is very surprising. A new explanation for this phenomenon is suggested here, involving a local effect at the entry point of the proton channel, rather than the long range effects suggested earlier.
authors.affiliation: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, SE-106 91 Stockholm, Sweden. Electronic address: ps@organ.su.se.
authors.forename: Per E M
authors.initials: PE
authors.lastname: Siegbahn
authors.affiliation: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, SE-106 91 Stockholm, Sweden.
authors.forename: Margareta R A
authors.initials: MR
authors.lastname: Blomberg
mesh_terms: D003576:Electron Transport Complex IV / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D008958:Models, Molecular; D009154:Mutation; D011487:Protein Conformation; D017494:Proton Pumps / Q000378:metabolism*
chemical_list: D017494:Proton Pumps; D003576:Electron Transport Complex IV
keywords: Computational modeling; Cytochrome oxidase; Proton pumping