title: Study of Anti Cancer Property of Scrophularia striata Extract on the Human Astrocytoma Cell Line (1321).
abstract: There are considerable efforts to identify naturally occurring substances as new drugs in cancer therapy. Many components of medicinal plants have been identified that possess substantial anticancerous properties. This prompted us to investigate the effect of Scrophularia striata (an Iranian species belonging to the Scrophulariace family) extract on the growth of astrocyte cancer cell line (1321). The 1321 cell line were seeded in 96-well culture plates in the presence and absence of various concentrations of either leaf and seed filtered and unfiltered extract of Scrophularia striata to determine their probable anticancer effects in comparison with etoposide (chemical anticancer reagent). filtered leaf extract of S. Striata showed strong anticancer effect on 1321cell line as compared to control group (cells not exposed to extracts), and even the group (adenocarcinoma gastric cell line) exposed to etoposide. Unlike the leaf extract, the seed extract activated cell proliferation in all experiments. Flow cytometry findings indicated that apoptosis is the mechanism by which the leaf extract inhibits cell proliferation. Our findings indicate that both leaves and seeds of S. Striata contain both anti cancer and cell growth enhancing agents.
authors.affiliation: Proteomics Research Center (PRC), Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Abdulreza
authors.initials: A
authors.lastname: Ardeshiry Lajimi
authors.affiliation: Proteomics Research Center (PRC), Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Rezaie-Tavirani
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Seyed Alireza
authors.initials: SA
authors.lastname: Mortazavi
authors.affiliation: Department of Cell and Molecular Biology, Khatam Universiy, Tehran, Iran.
authors.forename: Mansoureh
authors.initials: M
authors.lastname: Barzegar
authors.affiliation: Proteomics Research Center (PRC), Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Seyed Hasan
authors.initials: SH
authors.lastname: Moghadamnia
authors.affiliation: Institute of Forest and Rangelands, Tehran, Iran.
authors.forename: Mohamad Bagher
authors.initials: MB
authors.lastname: Rezaee
keywords: 1321 cell line; Anticancer effect; Astrocytoma; Flow cytometry; Scrophularia striata extract