title: In vivo hair growth-promoting effect of rice bran extract prepared by supercritical carbon dioxide fluid.
abstract: The potential hair growth-promoting activity of rice bran supercritical CO2 extract (RB-SCE) and major components of RB-SCE, linoleic acid, policosanol, γ-oryzanol, and γ-tocotrienol, were evaluated with the histological morphology and mRNA expression levels of cell growth factors using real-time reverse transcriptase-polymerase chain reaction (PCR) in C57BL/6 mice. RB-SCE showed hair growth-promoting potential to a similar extent as 3% minoxidil, showing that the hair follicles were induced to be in the anagen stage. The numbers of the hair follicles were significantly increased. In addition, mRNA expression levels of vascular endothelial growth factor (VEGF), insulin-like growth factor-1 (IGF-1), and keratinocyte growth factor (KGF) were also significantly increased and that of transforming growth factor-β (TGF-β) decreased in RB-SCE-treated groups. Among the major components of RB-SCE, linoleic acid and γ-oryzanol induced the formation of hair follicles according to examination of histological morphology and mRNA expression levels of cell growth factors. In conclusion, our results demonstrate that RB-SCE, particularly linoleic acid and γ-oryzanol, promotes hair growth and suggests RB-SCE can be applied as hair loss treatment.
authors.affiliation: RIS Center, IACF, Silla University.
authors.forename: Jae-Suk
authors.initials: JS
authors.lastname: Choi
authors.forename: Min-Hee
authors.initials: MH
authors.lastname: Jeon
authors.forename: Woi-Sook
authors.initials: WS
authors.lastname: Moon
authors.forename: Jin-Nam
authors.initials: JN
authors.lastname: Moon
authors.forename: Eun Jin
authors.initials: EJ
authors.lastname: Cheon
authors.forename: Joo-Wan
authors.initials: JW
authors.lastname: Kim
authors.forename: Sung Kyu
authors.initials: SK
authors.lastname: Jung
authors.forename: Yi-Hwa
authors.initials: YH
authors.lastname: Ji
authors.forename: Sang Wook
authors.initials: SW
authors.lastname: Son
authors.forename: Mi-Ryung
authors.initials: MR
authors.lastname: Kim
mesh_terms: D000505:Alopecia / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D051523:Fibroblast Growth Factor 7 / Q000235:genetics / Q000378:metabolism; D006197:Hair / Q000187:drug effects* / Q000254:growth & development; D018859:Hair Follicle / Q000187:drug effects*; D019787:Linoleic Acid / Q000494:pharmacology* / Q000627:therapeutic use; D051379:Mice; D008810:Mice, Inbred C57BL; D012275:Oryza / Q000737:chemistry*; D010666:Phenylpropionates / Q000494:pharmacology* / Q000627:therapeutic use; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D012333:RNA, Messenger / Q000378:metabolism; D012639:Seeds / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D010666:Phenylpropionates; D010936:Plant Extracts; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D042461:Vascular Endothelial Growth Factor A; D051523:Fibroblast Growth Factor 7; D019787:Linoleic Acid; C013172:gamma-oryzanol