title: Comparative studies of N-glycans and glycosaminoglycans present in SIRC (Statens Seruminstitut rabbit cornea) cells and corneal epithelial cells from rabbit eyes.
abstract: OBJECTIVE
  We compared cultured Statens Seruminstitut rabbit cornea (SIRC) cells and corneal epithelial cells from rabbit eyes by analyzing their N-glycans and glycosaminoglycans (GAGs). This work is a fundamental study on the efficacy of using cultured cells instead of animals for drug development.
  
  
  METHODS
  N-Glycans and GAGs from SIRC cell monolayers and corneal epithelial cells of rabbit eyes were analyzed by capillary electrophoresis (CE) and a combination of high-performance liquid chromatography (HPLC) and mass spectrometry.
  
  
  RESULTS
  High mannose-type glycans and a hybrid-type glycan were the common N-glycans in SIRC cells and corneal epithelial cells of rabbit eyes. Mono-fucosylated biantennary glycans with or without one N-acetylneuraminic acid residue were observed only in SIRC cells. Hyaluronic acid was the only measurable GAG in the corneal epithelial cells of rabbit eyes. In contrast, hyaluronic acid and chondroitin sulfates were abundantly present in SIRC cells.
  
  
  CONCLUSIONS
  Profiles of both N-glycans and GAGs were conspicuously different between SIRC cells and corneal epithelial cells of rabbit eyes. This report will be useful for the evaluation of pharmaceutical candidates when animals or cultured cells are employed in drug development studies.
authors.affiliation: Senju Pharmaceutical Co., LTD. , Nishiku, Kobe, Hyogo , Japan and.
authors.forename: Kinya
authors.initials: K
authors.lastname: Iwatsuka
authors.forename: Hiroki
authors.initials: H
authors.lastname: Iwamoto
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Kinoshita
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Inada
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Yasueda
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Kakehi
mesh_terms: D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003315:Cornea / Q000737:chemistry* / Q000166:cytology; D019075:Electrophoresis, Capillary; D019573:Epithelium, Corneal / Q000737:chemistry / Q000166:cytology; D006025:Glycosaminoglycans / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D011817:Rabbits
chemical_list: D006025:Glycosaminoglycans; D011134:Polysaccharides
keywords: Corneal epithelial cells; N-glycans; SIRC (Statens Seruminstitut rabbit cornea) cells; glycosaminoglycans