title: [Isolation, identification and characterization of five Pseudomonas strains].
abstract: OBJECTIVE
  We isolated Pseudomonas strains from soil samples collected from Changsha, Hunan province, catalogued them and studied the antimicrobial and antitumor activity.
  
  
  METHODS
  We isolated Pseudomonas strains from soil samples through Galleria bait method, identified and catalogued the isolated strains according to morphological observation, physiological and biochemical characteristics and the homologous analysis of 16S rRNA sequences of nucleotides. Antimicrobial, antagonistic fungi and anti-tumor activities were studied by diffusion plate assay, dual-culture assay and cytotoxicity test respectively.
  
  
  RESULTS
  We isolated 5 Pseudomonas strains from vegetable field and forest land of Changsha suburb, catalogued and named them as Pseudomonas protegens CY01, Pseudomonas chlororaphis CY02, Pseudomonas oryzihabitans CY04, Pseudomonas sp. CY05 and Pseudomonas putida CY06 respectively. P. protegens CY01 and P. chlororaphis CY02 have antibacterial activity against Bacillus subtillis and Staphylococcus aureus. P. chlororaphis CY02 has antagonistic activity against Pyricularia oryzae and anti-tumor cell activity against mouse melanoma B16 cells.
  
  
  CONCLUSIONS
  The isolated P. chlororaphis CY02 has a significant effect on pathogenic bacteria, Pyricularia oryzae and tumor cells.
authors.affiliation: State Key Laboratory Breeding Base of Microbial Molecular Biology, College of Life Science, Hunan Normal University, Changsha 410081, China. 2506660680@qq.com
authors.forename: Wangyue
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Life Science, Hunan University of Science and Technology, Xiangtan 411201, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.affiliation: State Key Laboratory Breeding Base of Microbial Molecular Biology, College of Life Science, Hunan Normal University, Changsha 410081, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: State Key Laboratory Breeding Base of Microbial Molecular Biology, College of Life Science, Hunan Normal University, Changsha 410081, China.
authors.forename: Youming
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory Breeding Base of Microbial Molecular Biology, College of Life Science, Hunan Normal University, Changsha 410081, China.
authors.forename: Shengbiao
authors.initials: S
authors.lastname: Hu
authors.affiliation: State Key Laboratory Breeding Base of Microbial Molecular Biology, College of Life Science, Hunan Normal University, Changsha 410081, China.
authors.forename: Lanlan
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory Breeding Base of Microbial Molecular Biology, College of Life Science, Hunan Normal University, Changsha 410081, China.
authors.forename: Yunjun
authors.initials: Y
authors.lastname: Sun
authors.affiliation: State Key Laboratory Breeding Base of Microbial Molecular Biology, College of Life Science, Hunan Normal University, Changsha 410081, China.
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Ding
authors.affiliation: State Key Laboratory Breeding Base of Microbial Molecular Biology, College of Life Science, Hunan Normal University, Changsha 410081, China.
authors.forename: Liqiu
authors.initials: L
authors.lastname: Xia
mesh_terms: D000818:Animals; D000898:Antibiosis; D001407:Bacillus / Q000502:physiology; D045744:Cell Line, Tumor; D051379:Mice; D003904:Mitosporic Fungi / Q000502:physiology; D008969:Molecular Sequence Data; D012275:Oryza / Q000382:microbiology; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D012988:Soil Microbiology; D013211:Staphylococcus aureus / Q000502:physiology