title: [Study on the extraction process and macroporous resin for purification of Timosaponin B II].
abstract: OBJECTIVE
  To optimize the extraction process and macroporous resin for purification of Timosaponin B II from Anemarrhena asphodeloides.
  
  
  METHODS
  Orthogonal design L9 (34) was employed to optimize the circumfluence extraction conditions by taking the extraction yield of Timosaponin B II as index. The absorption-desorption characteristics of eight kinds of macroporous resins were evaluated, then the best resin was chosen to optimize the purification process conditions.
  
  
  RESULTS
  The optimum extraction conditions were as follows: the herb was extracted for 2 times (2 hours each time) with 8.5-fold 50% ethanol at the first time and 6-fold 50% ethanol at the second time. HPD100 resin showed a good property for the absorption-desorption of Timosaponin B II. The optimum technological conditions of HPD100 resin were as follows:the solution concentration was 0.23 mg/mL, the amount of saturated adsorption at 4/5 body volumn (BV) resin, the HPD100 resin was washed with 3 BV water and 6 BV 20% ethanol solution to remove the impurity, then the Timosaponin B II was desorbed by 5 BV ethanol solution. The purity of Timosaponin B II was about 50%.
  
  
  CONCLUSIONS
  The optimized extraction process and purification is stable, efficient and suitable for industrial production.
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. lyp1066@hotmail.com
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Liu
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Zhen-Zhen
authors.initials: ZZ
authors.lastname: Cai
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Jian-Sheng
authors.initials: JS
authors.lastname: Tao
mesh_terms: D000042:Absorption; D031403:Anemarrhena / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification*; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification*; D013678:Technology, Pharmaceutical / Q000379:methods; D013997:Time Factors
chemical_list: D012117:Resins, Synthetic; D012503:Saponins; D013256:Steroids; C523027:timosaponin B-II; D000431:Ethanol