title: A recombinant PPRE-driven luciferase bioassay for identification of potential PPAR agonists.
abstract: A recombinant Huh7-PPRE-Luc cell line, carrying a peroxisome proliferator response element (PPRE)-driven luciferase gene, is an efficient tool for evaluation of potential peroxisome proliferator-activated receptor (PPAR) agonists. The cells exhibited a good dose-response induction in PPRE-driven luciferase activity by three subtypes of PPAR agonists as well as by a retinoid X receptor agonist, 9-cis-retinoic acid. Here, the bioassay is fitted into a 96-well plate format for high-throughput screening purpose.
authors.affiliation: Laboratory of Molecular Toxicology, Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Zhunan, Miaoli, Taiwan.
authors.forename: Feng-Yuan
authors.initials: FY
authors.lastname: Tsai
authors.affiliation: Laboratory of Molecular Toxicology, Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Zhunan, Miaoli, Taiwan.
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Cheng
authors.affiliation: Laboratory of Molecular Toxicology, Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Zhunan, Miaoli, Taiwan. Electronic address: tctsou@nhri.org.tw.
authors.forename: Tsui-Chun
authors.initials: TC
authors.lastname: Tsou
mesh_terms: D002460:Cell Line; D002999:Clone Cells; D055808:Drug Discovery / Q000379:methods*; D052244:Endocrine Disruptors / Q000494:pharmacology; D017930:Genes, Reporter / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000378:metabolism; D057166:High-Throughput Screening Assays; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D047492:Peroxisome Proliferator-Activated Receptors / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D020025:Peroxisome Proliferators / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D020218:Response Elements / Q000187:drug effects*; D013937:Thymidine Kinase / Q000235:genetics / Q000378:metabolism
chemical_list: D052244:Endocrine Disruptors; D047492:Peroxisome Proliferator-Activated Receptors; D020025:Peroxisome Proliferators; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D008156:Luciferases; D013937:Thymidine Kinase
keywords: High-throughput screening; Luciferase reporter gene; PPAR agonists; PPRE promoter assay; RXR agonists