title: High glucose condition suppresses neurosphere formation by human periodontal ligament-derived mesenchymal stem cells.
abstract: D-Glucose serves many roles in cellular functions, but its role in human periodontal ligament-derived mesenchymal stem cells (hPSLSCs) is yet unknown. Here, the roles of high glucose concentration on neurogenic differentiation by hPDLSCs were investigated. Two-stage neurogenic induction protocol was employed. Cells were maintained in normal neurogenic induction medium, high glucose condition, or high mannose condition. The results showed that high glucose attenuated neurosphere formation efficiency by hPDLSCs in terms of morphology, neurogenic marker expression, without a deleterious effect on cell viability. Contrastingly, neurosphere-derived cells matured in high glucose condition exhibited normal neuronal characteristics compared to the control. During neurosphere formation in high glucose, glucose transporters (GLUTs) mRNA levels were significantly decreased, corresponding with the deprivation of cellular glucose uptake. Further, a glucose uptake inhibitor, cytochalasin B, was used to confirm the deleterious effects of glucose uptake deprivation during neurosphere formation. The results demonstrated that deprivation of glucose uptake attenuated neurosphere formation efficiency by hPDLSCs. Together, the results illustrated that high glucose condition attenuated the efficiency of neurosphere formation but not neuronal maturation, which may occur through the downregulation of GLUTs and the reduction of glucose uptake.
authors.affiliation: Department of Pharmacology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand; Research Unit of Mineralized Tissue, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330, Thailand; Graduate Program in Veterinary Bioscience, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand.
authors.forename: Chenphop
authors.initials: C
authors.lastname: Sawangmake
authors.forename: Prasit
authors.initials: P
authors.lastname: Pavasant
authors.forename: Piyarat
authors.initials: P
authors.lastname: Chansiripornchai
authors.forename: Thanaphum
authors.initials: T
authors.lastname: Osathanon
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D051246:Glucose Transport Proteins, Facilitative / Q000096:biosynthesis; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D010513:Periodontal Ligament / Q000166:cytology; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D051246:Glucose Transport Proteins, Facilitative; D012333:RNA, Messenger; D005947:Glucose
keywords: GLUCOSE; NEURONAL DIFFERENTIATION; PERIODONTAL LIGAMENT-DERIVED MESENCHYMAL STEM CELLS (hPDLSCs)