title: Preparative separation and purification of rosmarinic acid from perilla seed meal via combined column chromatography.
abstract: In this study, the preparative separation and purification of rosmarinic acid (RA) from perilla seed meal (PSM), which is a by-product of edible oil production, was achieved using combined column chromatography over macroporous and polyamide resins. To optimize the RA enrichment process, the performance and separation characteristics of nine selected macroporous resins with different chemical and physical properties were investigated. SP825 resin was the most effective: the content of RA increased from 0.27% in the original extract to 16.58% in the 50% ethanol fraction (a 61.4-fold increase). During further purification treatment on polyamide resin, 90.23% pure RA could be obtained in the 70% ethanol fraction. RA with a higher purity (>95%) could also be easily obtained using one crystallization operation. The proposed method is simple, easily operated, cost-effective, and environmentally friendly and is suitable for both large-scale RA production and waste management.
authors.affiliation: Key Laboratory of Structure-Based Drug Design & Discovery Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China; Department of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Weizhuo
authors.initials: W
authors.lastname: Tang
authors.affiliation: Department of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China; INIA Dois Portos, Instituto Nacional de Investigação Agrária e Veterinária, I.P.2565-191 Dois Portos, Portugal.
authors.forename: Baoshan
authors.initials: B
authors.lastname: Sun
authors.affiliation: Key Laboratory of Structure-Based Drug Design & Discovery Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China; Department of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address: zyq4885@126.com.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D002934:Cinnamates / Q000737:chemistry / Q000302:isolation & purification*; D053630:Depsides / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D031343:Perilla / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D012117:Resins, Synthetic / Q000737:chemistry; D012639:Seeds / Q000737:chemistry*; D000097123:Rosmarinic Acid
chemical_list: D002934:Cinnamates; D053630:Depsides; D010936:Plant Extracts; D012117:Resins, Synthetic; D000097123:Rosmarinic Acid
keywords: Combination chromatography; Macroporous resin; Perilla seed meal; Polyamide resin; Rosmarinic acid