title: Metabolically engineered glucose-utilizing Shewanella strains under anaerobic conditions.
abstract: Comparative genome analysis of Shewanella strains predicted that the strains metabolize preferably two- and three-carbon carbohydrates as carbon/electron source because many Shewanella genomes are deficient of the key enzymes in glycolysis (e.g., glucokinase). In addition, all Shewanella genomes are known to have only one set of genes associated with the phosphotransferase system required to uptake sugars. To engineer Shewanella strains that can utilize five- and six-carbon carbohydrates, we constructed glucose-utilizing Shewanella oneidensis MR-1 by introducing the glucose facilitator (glf; ZMO0366) and glucokinase (glk; ZMO0369) genes of Zymomonas mobilis. The engineered MR-1 strain was able to grow on glucose as a sole carbon/electron source under anaerobic conditions. The glucose affinity (Ks) and glucokinase activity in the engineered MR-1 strain were 299.46 mM and 0.259 ± 0.034 U/g proteins. The engineered strain was successfully applied to a microbial fuel cell system and exhibited current generation using glucose as the electron source.
authors.affiliation: School of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan gwagi-ro, Buk-gu, Gwangju 500-712, Republic of Korea.
authors.forename: Donggeon
authors.initials: D
authors.lastname: Choi
authors.affiliation: School of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan gwagi-ro, Buk-gu, Gwangju 500-712, Republic of Korea.
authors.forename: Sae Bom
authors.initials: SB
authors.lastname: Lee
authors.affiliation: College of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-gu, Seoul 136-701, Republic of Korea.
authors.forename: Sohyun
authors.initials: S
authors.lastname: Kim
authors.affiliation: College of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-gu, Seoul 136-701, Republic of Korea.
authors.forename: Byoungnam
authors.initials: B
authors.lastname: Min
authors.affiliation: College of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-gu, Seoul 136-701, Republic of Korea. Electronic address: igchoi@korea.ac.kr.
authors.forename: In-Geol
authors.initials: IG
authors.lastname: Choi
authors.affiliation: School of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan gwagi-ro, Buk-gu, Gwangju 500-712, Republic of Korea. Electronic address: ischang@gist.ac.kr.
authors.forename: In Seop
authors.initials: IS
authors.lastname: Chang
mesh_terms: D000332:Aerobiosis / Q000187:drug effects; D000693:Anaerobiosis / Q000187:drug effects; D001674:Bioelectric Energy Sources / Q000382:microbiology; D004560:Electricity; D005941:Glucokinase / Q000378:metabolism; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D060847:Metabolic Engineering / Q000379:methods*; D020592:Shewanella / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*
chemical_list: D005941:Glucokinase; D005947:Glucose
keywords: Anaerobic conditions; Current generation; Glucose utilization; Microbial fuel cells; Shewanella oneidensis MR-1