title: A protein profile of visceral adipose tissues linked to early pathogenesis of type 2 diabetes mellitus.
abstract: Adipose tissue is increasingly recognized as an endocrine organ playing important pathophysiological roles in metabolic abnormalities, such as obesity, cardiovascular disease, and type 2 diabetes mellitus (T2DM). In particular, visceral adipose tissue (VAT), as opposed to subcutaneous adipose tissue, is closely linked to the pathogenesis of insulin resistance and T2DM. Despite the importance of VAT, its molecular signatures related to the pathogenesis of T2DM have not been systematically explored. Here, we present comprehensive proteomic analysis of VATs in drug-naïve early T2DM patients and subjects with normal glucose tolerance. A total of 4,707 proteins were identified in LC-MS/MS experiments. Among them, 444 increased in abundance in T2DM and 328 decreased. They are involved in T2DM-related processes including inflammatory responses, peroxisome proliferator-activated receptor signaling, oxidative phosphorylation, fatty acid oxidation, and glucose metabolism. Of these proteins, we selected 11 VAT proteins that can represent alteration in early T2DM patients. Among them, up-regulation of FABP4, C1QA, S100A8, and SORBS1 and down-regulation of ACADL and PLIN4 were confirmed in VAT samples of independent early T2DM patients using Western blot. In summary, our profiling provided a comprehensive basis for understanding the link of a protein profile of VAT to early pathogenesis of T2DM.
authors.affiliation: Department of Chemistry, Research Institute for Natural Sciences, Korea University, Seoul, 136-701, Republic of Korea;
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Sehyun
authors.initials: S
authors.lastname: Chae
authors.forename: Hokeun
authors.initials: H
authors.lastname: Kim
authors.forename: Dong-Gi
authors.initials: DG
authors.lastname: Mun
authors.forename: Seunghoon
authors.initials: S
authors.lastname: Back
authors.forename: Hye Yeon
authors.initials: HY
authors.lastname: Choi
authors.forename: Kyong Soo
authors.initials: KS
authors.lastname: Park
authors.forename: Daehee
authors.initials: D
authors.lastname: Hwang
authors.forename: Sung Hee
authors.initials: SH
authors.lastname: Choi
authors.forename: Sang-Won
authors.initials: SW
authors.lastname: Lee
mesh_terms: D002853:Chromatography, Liquid; D030562:Databases, Protein; D003924:Diabetes Mellitus, Type 2 / Q000209:etiology* / Q000378:metabolism* / Q000503:physiopathology; D006801:Humans; D050152:Intra-Abdominal Fat / Q000378:metabolism* / Q000473:pathology; D013058:Mass Spectrometry; D008954:Models, Biological; D008970:Molecular Weight; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D015398:Signal Transduction
chemical_list: D020543:Proteome