title: Improvement of direct ethanol fermentation from woody biomasses by the Antarctic basidiomycetous yeast, Mrakia blollopis, under a low temperature condition.
abstract: The Antarctic basidiomycetous yeast Mrakia blollopis SK-4 can quite uniquely ferment various sugars under low temperature conditions. When strain SK-4 fermented lignocellulosic biomass using the direct ethanol fermentation (DEF) technique, approximately 30% to 65% of the theoretical ethanol yield was obtained without and with the addition of the non-ionic surfactant Tween 80, respectively. Therefore, DEF from lignocellulosic biomass with M. blollopis SK-4 requires the addition of a non-ionic surfactant to improve fermentation efficiency. DEF with lipase converted Eucalyptus and Japanese cedar to 12.6g/l, and 14.6g/l ethanol, respectively. In the presence of 1% (v/v) Tween 80 and 5U/g-dry substrate lipase, ethanol concentration increased about 1.4- to 2.4-fold compared to that without Tween 80 and lipase. We therefore consider that the combination of M. blollopis SK-4 and DEF with Tween 80 and lipase has good potential for ethanol fermentation in cold environments.
authors.affiliation: Biomass Refinery Research Center (BRRC), National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan. Electronic address: spindletuber@gmail.com.
authors.forename: Masaharu
authors.initials: M
authors.lastname: Tsuji
authors.affiliation: Bio-production Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-higashi, Toyohira-ku, Sapporo, Hokkaido 062-8517, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yokota
authors.affiliation: National Institute of Polar Research (NIPR), 10-3 Midori-cho, Tachikawa, Tokyo 190-8518, Japan.
authors.forename: Sakae
authors.initials: S
authors.lastname: Kudoh
authors.affiliation: Biomass Refinery Research Center (BRRC), National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan; Graduate School of Life Science, Hokkaido University, N10W8 Kita-ku, Sapporo, Hokkaido 060-0810, Japan. Electronic address: tamotsu.hoshino@aist.go.jp.
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Hoshino
mesh_terms: D000864:Antarctic Regions; D001487:Basidiomycota / Q000378:metabolism*; D018533:Biomass; D003080:Cold Temperature; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000502:physiology*; D014934:Wood / Q000378:metabolism*
chemical_list: D000431:Ethanol
keywords: Antarctica; Cryophilic yeast; Direct ethanol fermentation; Ethanol fermentation; Woody biomass