title: Transcriptomic microarray analysis of BoMac cells after infection with bovine foamy virus.
abstract: Bovine foamy virus (BFV) infections are highly prevalent among cattle worldwide. However, relatively little is known about the impact of this virus on the host immune system. In our study, we focused on a bovine macrophage cell line (BoMac) and examined changes in the BoMac transcriptome after in vitro infection with BFV using bovine BLOPlus oligo microarrays. One hundred twenty-four genes showed significant changes in expression level. The biological process categories found to be enriched include metabolic processes, cell communication, transport, immune system processes, and response to extracellular stimuli. RT-qPCR was applied to confirm the results obtained for representative genes.
authors.affiliation: Department of Biochemistry, National Veterinary Research Institute, Partyzantów 57, 24-100, Pulawy, Poland, mrolka@piwet.pulawy.pl.
authors.forename: Marzena
authors.initials: M
authors.lastname: Rola-Łuszczak
authors.forename: Magdalena
authors.initials: M
authors.lastname: Materniak
authors.forename: Aneta
authors.initials: A
authors.lastname: Pluta
authors.forename: Marcel
authors.initials: M
authors.lastname: Hulst
authors.forename: Jacek
authors.initials: J
authors.lastname: Kuźmak
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D008264:Macrophages / Q000276:immunology* / Q000821:virology*; D046228:Microarray Analysis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016092:Spumavirus / Q000254:growth & development* / Q000276:immunology*