title: Characteristics of established KSG cells derived from the scorpionfish Sebastiscus marmoratus: what happens under the hydrostatic pressure like the deep sea?
abstract: Advances in cell biology depend, partly, on the development of new cell lines and culture methods. Our research focused on a fibroblast-like cell line, "KSG," which is derived from scorpionfish fin tissue (Sebastiscus marmoratus). Cells were grown in Leibovitz's L-15 medium with 10% fetal bovine serum following standard procedures. The optimum growth temperatures for these lines ranged from 15°C to 25°C. All cells survived storage for at least 3 yr at -80°C. Subsequently, they were continuously cultured until the 78th generation without evident changes in their morphology. Moreover, we were able to culture KSG cells in the absence of fetal bovine serum in a culture medium containing the fish serum "SeaGrow." Optimum SeaGrow concentrations for these cells ranged from 5% to 20%. The growth rate of KSG cells decreased when the concentration of SeaGrow was reduced to 1%. However, this decrease could be partially reversed by adding 0.5% "Hy-Fish." In addition, the inclusion of Hy-Fish improved cell adhesion. KSG cells that were cultured in serum-free culture media containing 0.5% and 1% Hy-Fish had been added and were able to survive at low densities. Furthermore, we successfully transfected this cell line with a commercial plasmid vector coding a fluorescent protein using the cationic lipid. Finally, the analyses of cell behavior under hydrostatic pressure showed that some pressures (10 MPa) helped the cells to proliferate more.
authors.affiliation: Marine Bioresource Exploration Research Team (MBE), Marine Biodiversity Research Program, Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, 237-0061, Japan, tsuruwaka@jamstec.go.jp.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tsuruwaka
authors.forename: Eriko
authors.initials: E
authors.lastname: Shimada
authors.forename: Makiko
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hatada
mesh_terms: D058500:Animal Fins / Q000166:cytology*; D000818:Animals; D016895:Culture Media, Serum-Free; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology; D006874:Hydrostatic Pressure; D066298:In Vitro Techniques; D009792:Oceans and Seas; D010473:Perciformes; D013696:Temperature
chemical_list: D016895:Culture Media, Serum-Free