title: Fibrillary astrocytes are decreased in the subgenual cingulate in schizophrenia.
abstract: Decreases in astrocyte density and in glial fibrillary acid protein (GFAP) mRNA in the anterior cingulate cortex have been reported changed in mood and affective disorders. Our study examines the relative density and frequency of fibrillary and gemistocytic astrocytes in the white matter of the subgenual cingulate cortex in 11 schizophrenia, 16 bipolar disorder, 20 major depression and 20 normal control cases. Serial coronal sections were stained with H&E for anatomical guidance and GFAP immunohistochemistry for astrocyte identification. Astrocyte density was measured using systematic anatomical distinctions and randomised counting methods previously reported. Astrocytes were classified as fibrillary or gemistocytic based on staining and morphometric criteria and were measured in the crown and base of the gyral white matter. Fibrillary astrocytes were decreased in the base of the cingulate white matter in schizophrenia (p = 0.046), with no change in the density of gemistocytic astrocytes. There was no change in density of gemistocytic astrocytes. This suggests that the previously reported decrease in astrocytes in schizophrenia in the subgenual cingulate is accounted for only by a change in fibrillary astrocytes. With recent findings suggesting fibrillary astrocytes regulate synaptic glutamate this morphological change may relate to disregulation of function of the subgenual cingulate cortex.
authors.affiliation: Neuropathology Unit, Charing Cross Hospital, Imperial College London, London, W6 8RF, UK, Matthew.r.williams@imperial.ac.uk.
authors.forename: Matthew
authors.initials: M
authors.lastname: Williams
authors.forename: Ronald K B
authors.initials: RK
authors.lastname: Pearce
authors.forename: Steven R
authors.initials: SR
authors.lastname: Hirsch
authors.forename: Olaf
authors.initials: O
authors.lastname: Ansorge
authors.forename: Maria
authors.initials: M
authors.lastname: Thom
authors.forename: Michael
authors.initials: M
authors.lastname: Maier
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001253:Astrocytes / Q000378:metabolism*; D002452:Cell Count; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D006179:Gyrus Cinguli / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D019964:Mood Disorders / Q000473:pathology; D012559:Schizophrenia / Q000473:pathology*; D055815:Young Adult
chemical_list: D005904:Glial Fibrillary Acidic Protein