title: Expression of modulators of extracellular matrix structure after anterior cruciate ligament injury.
abstract: The ability of the anterior cruciate ligament (ACL) to heal after injury declines within the first 2 weeks after ACL rupture. To begin to explore the mechanism behind this finding, we quantified the expression of genes for collagen I and III, decorin, tenascin-C, and alpha smooth muscle actin, as well as matrix metalloproteinase (MMP)-1 and -13 gene expression within multiple tissues of the knee joint after ACL injury in a large animal model over a 2-week postinjury period. Gene expression of collagen I and III, decorin, and MMP-1 was highest in the synovium, whereas the highest MMP-13 gene expression levels were found in the ACL. The gene expression for collagen and decorin increased over the 2 weeks to levels approaching that in the ligament and synovium; however, no significant increase in either of the MMPs was found in the provisional scaffold. This suggests that although the ACL and synovium up-regulate both anabolic and catabolic factors, the provisional scaffold is primarily anabolic in function. The relative lack of provisional scaffold formation within the joint environment may thus be one of the key reasons for ACL degradation after injury.
authors.affiliation: Department of Orthopaedic Surgery, Boston Children's Hospital, Boston, Massachusetts.
authors.forename: Carla M
authors.initials: CM
authors.lastname: Haslauer
authors.forename: Benedikt L
authors.initials: BL
authors.lastname: Proffen
authors.forename: Victor M
authors.initials: VM
authors.lastname: Johnson
authors.forename: Martha M
authors.initials: MM
authors.lastname: Murray
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000201:enzymology / Q000473:pathology; D000070598:Anterior Cruciate Ligament Injuries; D003094:Collagen / Q000378:metabolism*; D058575:Decorin / Q000378:metabolism*; D005109:Extracellular Matrix / Q000235:genetics / Q000473:pathology*; D015971:Gene Expression Regulation, Enzymologic; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism; D013552:Swine; D013556:Swine, Miniature; D013583:Synovial Membrane / Q000201:enzymology; D019063:Tenascin / Q000378:metabolism*; D014945:Wound Healing / Q000235:genetics
chemical_list: D058575:Decorin; D019063:Tenascin; D003094:Collagen; D053509:Matrix Metalloproteinase 13; D020781:Matrix Metalloproteinase 1