title: The evaluation of the amebicidal activity of Eryngium planum extracts.
abstract: Selected fractions of ethanolic extracts obtained from leaves and roots of Eryngium planum (Apiaceae) were evaluated in vitro for amebicidal activity against Acanthamoeba castellanii. This free-living ameba is the cause of Acanthamoeba keratitis, which is a painful, vision-impairing disease of the eyes, and chronic granulomatous amebic encephalitis. Treatment is very difficult and not always effective because of encystation, which makes the amebae highly resistant to anti-amebic drugs. The search for novel natural amebicidal agents is still of current interest. Fractions of E. planum ethanolic extract from basal leaves: flavonoid fraction (Lf), flavonoid-saponin fraction (Lf-s), saponin fraction (Ls) and phenolic acids fraction (La) and from roots: saponin fraction (Rs) and phenolic acids fraction (Ra) were assayed for antiamebic activity. In the presence of the saponin fractions and phenolic acid fractions (ranging from 1-5 mg/mL), the number of the trofozoites of Acanthamoeba castellanii viable strain 309 decreased during the experimental period (0-72 h). On the other hand, the flavonoid fraction from leaves showed a stimulating activity on the amebae. Almost all fractions (except the flavonoid fraction) showed a time- and dose-dependent amebistatic activity on the trophozoites. Of the fractions tested, the phenolic acid fraction from roots at the concentration of 5 mg/L showed the amebicidal activity on the trophozoites.
authors.affiliation: Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, 10 Fredry St., 61-701 Poznań, Poland. mderda@ump.edu.pl
authors.forename: Monika
authors.initials: M
authors.lastname: Derda
authors.affiliation: Department of Pharmaceutical Botany, Poznan University of Medical Sciences, 14 Sw. Marii Magdaleny St., 61-861 Poznań, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Thiem
authors.affiliation: Department of Pharmaceutical Botany, Poznan University of Medical Sciences, 14 Sw. Marii Magdaleny St., 61-861 Poznań, Poland.
authors.forename: Jaromir
authors.initials: J
authors.lastname: Budzianowski
authors.affiliation: Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, 10 Fredry St., 61-701 Poznań, Poland.
authors.forename: Waldemar Jerzy
authors.initials: WJ
authors.lastname: Wojt
authors.affiliation: Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, 10 Fredry St., 61-701 Poznań, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Wojtkowiak-Giera
mesh_terms: D048248:Acanthamoeba castellanii / Q000187:drug effects* / Q000254:growth & development; D000563:Amebicides / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D031049:Eryngium / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D062385:Hydroxybenzoates / Q000302:isolation & purification / Q000494:pharmacology; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves; D018517:Plant Roots; D010946:Plants, Medicinal; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology; D012997:Solvents / Q000737:chemistry; D013997:Time Factors
chemical_list: D000563:Amebicides; D005419:Flavonoids; D062385:Hydroxybenzoates; D010936:Plant Extracts; D012503:Saponins; D012997:Solvents; D000431:Ethanol; C017616:phenolic acid