title: Characterization of Streptococcus pneumoniae invasive serotype 19A isolates recovered in Colombia.
abstract: The aim of this study was to determine the molecular characterization of invasive penicillin non-susceptible Streptococcus pneumoniae serotype 19A isolates, collected in Colombia between 1994 and 2012. A total of 115 isolates serotype 19A were analyzed. Genetic relationship of 80 isolates with minimal inhibitory concentration (MIC) to penicillin ≥0.125 μg/was determined by pulsed-field gel electrophoresis (PFGE) and selected strains were studied by multilocus sequence typing (MLST). Among the 115 isolates, resistance to penicillin in meningitis was 64.2%, in non-meningitis 32.2% were intermediate and 1.1% were high resistance. The most frequent sequence types were ST320 (33.7%), ST276 (21.5%), and ST1118 (11.2%). Five isolates were associated with the Spain(9V)-ST156 clone, and two isolates were related to Colombia(23F)-ST338 clone. S. pneumoniae serotype 19A increased in Colombia was associated with the spread of isolates genetically related to ST320 and ST276, and emergence of capsular variants of worldwide-disseminated clones.
authors.affiliation: Grupo de Microbiología. Instituto Nacional de Salud, Avenida calle 26 No. 51-20, Bogotá, Colombia.
authors.forename: Viviana
authors.initials: V
authors.lastname: Ramos
authors.affiliation: Grupo de Microbiología. Instituto Nacional de Salud, Avenida calle 26 No. 51-20, Bogotá, Colombia.
authors.forename: Eliana L
authors.initials: EL
authors.lastname: Parra
authors.affiliation: Grupo de Microbiología. Instituto Nacional de Salud, Avenida calle 26 No. 51-20, Bogotá, Colombia.
authors.forename: Carolina
authors.initials: C
authors.lastname: Duarte
authors.affiliation: Grupo de Microbiología. Instituto Nacional de Salud, Avenida calle 26 No. 51-20, Bogotá, Colombia. Electronic address: jmoreno@ins.gov.co.
authors.forename: Jaime
authors.initials: J
authors.lastname: Moreno
mesh_terms: D003105:Colombia; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D010403:Penicillin Resistance; D011008:Pneumococcal Infections / Q000382:microbiology*; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000302:isolation & purification
keywords: Clonal complexes; Multidrug resistance; Streptococcus pneumoniae serotype 19A isolates