title: Highlights on novel technologies for the detection of antibodies to Ro60, Ro52, and SS-B.
abstract: OBJECTIVE
  We aimed to compare a chemiluminescent immunoassay (CIA, QUANTA Flash) on BIO-FLASH with a multiplex flow immunoassay (MFI) on BioPlex 2200 for the detection of antibodies to Ro60, Ro52, and SS-B.
  
  
  METHODS
  The study included 241 samples, from patients suffering from systemic autoimmune diseases (n = 108) as well as disease controls (n = 133). All samples were tested for anti-Ro52, anti-Ro60, and anti-SS-B (La) antibodies on QUANTA Flash (INOVA Diagnostics, San Diego, USA) and BioPlex 2200 (Bio-Rad Laboratories Inc., Hercules, USA). Discrepant samples were tested by two independent methods: BlueDot/ANA and QUANTRIX Microarray (both D-tek, Belgium).
  
  
  RESULTS
  The overall qualitative agreements were 95.4% (95% confidence interval, CI 92.0-97.7%) for anti-Ro52, 98.8% (95% CI 96.4-99.7%) for anti-Ro60, and 91.7% (95% CI 87.5-94.9%) for anti-SS-B antibodies. There were 34 discrepant samples among all assays (20 anti-SS-B, 11 anti-Ro52, 3 anti-Ro60). 30/33 of retested samples (by D-tek dot blot) agreed with the QUANTA Flash results. Similar findings were obtained with QUANTRIX Microarray kit.
  
  
  CONCLUSIONS
  QUANTA Flash and BioPlex 2200 show good qualitative agreement. The clinical performances were similar for anti-Ro52 and anti-Ro60 autoantibodies while differences were observed for anti-SS-B (La) antibodies.
authors.affiliation: Immunology and Allergology Laboratory Unit, Azienda Sanitaria di Firenze, San Giovanni di Dio Hospital, Florence, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Infantino
authors.affiliation: Department of Research, INOVA Diagnostics Inc., 9900 Old Grove Road, San Diego, CA 92131-1638, USA.
authors.forename: C
authors.initials: C
authors.lastname: Bentow
authors.affiliation: Department of Research, INOVA Diagnostics Inc., 9900 Old Grove Road, San Diego, CA 92131-1638, USA.
authors.forename: A
authors.initials: A
authors.lastname: Seaman
authors.affiliation: Rheumatology Unit, Department of Internal Medicine, San Giovanni di Dio Hospital, Azienda Sanitaria di Firenze, Florence, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Benucci
authors.affiliation: Rheumatology Unit, L. Sacco University Hospital, Milan, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Atzeni
authors.affiliation: Rheumatology Unit, L. Sacco University Hospital, Milan, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Sarzi-Puttini
authors.affiliation: Immunology and Allergology Laboratory Unit, Azienda Sanitaria di Firenze, San Giovanni di Dio Hospital, Florence, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Olivito
authors.affiliation: Immunology and Allergology Laboratory Unit, Azienda Sanitaria di Firenze, San Giovanni di Dio Hospital, Florence, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Meacci
authors.affiliation: Immunology and Allergology Laboratory Unit, Azienda Sanitaria di Firenze, San Giovanni di Dio Hospital, Florence, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Manfredi
authors.affiliation: Department of Research, INOVA Diagnostics Inc., 9900 Old Grove Road, San Diego, CA 92131-1638, USA.
authors.forename: M
authors.initials: M
authors.lastname: Mahler
mesh_terms: D001323:Autoantibodies / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D001327:Autoimmune Diseases / Q000175:diagnosis / Q000276:immunology; D015200:Fluoroimmunoassay / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012261:Ribonucleoproteins / Q000276:immunology*; D012680:Sensitivity and Specificity; D000097585:SS-B Antigen; D000099207:SS-A Antigen
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D012261:Ribonucleoproteins; D000097585:SS-B Antigen; D000099207:SS-A Antigen