title: Agonist of inward rectifier K+ channels enhances the protection of ischemic postconditioning in isolated rat hearts.
abstract: BACKGROUND
  Selective inhibition of inward rectifier K+channels could abolish the protection mediated by ischemic preconditioning, but the roles of these channels in ischemic postconditioning have not been well characterized. Our study aims to evaluate the effect of inward rectifier K+channels on the protection induced by ischemic postconditioning.
  
  
  METHODS
  Langendorff-perfused rat hearts (n=8 per group) were split into four groups: postconditioning hearts (IPO group); ischemic postconditioning with BaCl2hearts (PB group); ischemic postconditioning with zacopride hearts (PZ group); and without ischemic postconditioning (CON group). After suffering 30 minutes of global ischemia, groups IPO, PB and PZ went through 10 seconds of ischemic postconditioning with three different perfusates: respectively, Krebs-Henseleit buffer (IPO group); 20 μmol/L BaCl2(antagonist of the channel, PB group); 1 μmol/L zacopride (agonist of the channel, PZ group).
  
  
  RESULTS
  At the end of reperfusion, the myocardial performance was better preserved in the PZ group than the other three groups. The PB group showed no significant differences from the CON group.
  
  
  CONCLUSIONS
  Our study has shown that the IK1channel agonist zacopride is associated with the enhancement of ischemic postconditioning.
authors.affiliation: Department of Cardiopulmonary Bypass, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China.
authors.forename: Z
authors.initials: Z
authors.lastname: Liao
authors.affiliation: Department of Cardiopulmonary Bypass, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China fwzyfeng@gmail.com.
authors.forename: Z
authors.initials: Z
authors.lastname: Feng
authors.affiliation: Department of Cardiopulmonary Bypass, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China.
authors.forename: C
authors.initials: C
authors.lastname: Long
keywords: Langendorff; cardioprotection; inward rectifier K+ channels, zacopride; ischemic postconditioning