title: Glycan analysis of Prostate Specific Antigen (PSA) directly from the intact glycoprotein by HR-ESI/TOF-MS.
abstract: Glycans are important modulators of the biological function of proteins and are normally characterized from proteolytic glycopeptides or from (N-)glycans released enzymatically by glycosidase treatment or chemically by hydrazinolysis. We demonstrate that glycan compositions can easily be determined directly by LC-ESI/TOF-MS from intact glycoproteins even with a very complex glycosylation pattern. Interpretation of isotopically resolved mass spectra of prostate specific antigen (PSA) using bioinformatics tools gives within a few hours the glycan compositions of 38 glycoforms.
authors.affiliation: Organic Chemistry, Department of Chemistry, University of Hamburg , Martin-Luther-King Platz 6, 20144 Hamburg, Germany.
authors.forename: Henning N
authors.initials: HN
authors.lastname: Behnken
authors.forename: Alexandra
authors.initials: A
authors.lastname: Ruthenbeck
authors.forename: Jan-Mirco
authors.initials: JM
authors.lastname: Schulz
authors.forename: Bernd
authors.initials: B
authors.lastname: Meyer
mesh_terms: D002853:Chromatography, Liquid; D006023:Glycoproteins / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis*; D017430:Prostate-Specific Antigen / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D006023:Glycoproteins; D011134:Polysaccharides; D017430:Prostate-Specific Antigen