title: 1,8-cineole (eucalyptol) mitigates inflammation in amyloid Beta toxicated PC12 cells: relevance to Alzheimer's disease.
abstract: Inflammatory process has a fundamental role in the pathogenesis of Alzheimer's disease and insoluble amyloid beta deposits and neurofibrillary tangles provide the obvious stimuli for inflammation. The present study demonstrate the effect of pretreatment of 1,8-cineole (Cin) on inflammation induced by Aβ(25-35) in differentiated PC12 cells. The cells were treated with Cin at different doses for 24 h and then replaced by media containing Aβ(25-35) for another 24 h. The cell viability was decreased in Aβ(25-35) treated cells which was significantly restored by Cin pretreatment. Cin successfully reduced the mitochondrial membrane potential, ROS and NO levels in Aβ(25-35) treated cells. Cin also lowered the levels of proinflammatory cytokines TNF-α, IL-1β and IL-6 in Aβ(25-35) treated cells. Moreover, Cin also succeeded in lowering the expression of NOS-2, COX-2 and NF-κB. This study suggests the protective effects of Cin on inflammation and provides additional evidence for its potential beneficial use in therapy as an anti-inflammatory agent in neurodegenerative disease.
authors.affiliation: Neurotoxicology Laboratory, Department of Medical Elementology and Toxicology, Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi, 110062, India.
authors.forename: Andleeb
authors.initials: A
authors.lastname: Khan
authors.forename: Kumar
authors.initials: K
authors.lastname: Vaibhav
authors.forename: Hayate
authors.initials: H
authors.lastname: Javed
authors.forename: Rizwana
authors.initials: R
authors.lastname: Tabassum
authors.forename: Md Ejaz
authors.initials: ME
authors.lastname: Ahmed
authors.forename: Mohd Moshahid
authors.initials: MM
authors.lastname: Khan
authors.forename: M Badruzzaman
authors.initials: MB
authors.lastname: Khan
authors.forename: Pallavi
authors.initials: P
authors.lastname: Shrivastava
authors.forename: Farah
authors.initials: F
authors.lastname: Islam
authors.forename: M Saeed
authors.initials: MS
authors.lastname: Siddiqui
authors.forename: M M
authors.initials: MM
authors.lastname: Safhi
authors.forename: Fakhrul
authors.initials: F
authors.lastname: Islam
mesh_terms: D000544:Alzheimer Disease / Q000473:pathology*; D016229:Amyloid beta-Peptides / Q000502:physiology; D000818:Animals; D003511:Cyclohexanols / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D000077591:Eucalyptol; D007249:Inflammation / Q000517:prevention & control*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D039821:Monoterpenes / Q000494:pharmacology*; D018384:Oxidative Stress; D016716:PC12 Cells; D010446:Peptide Fragments / Q000502:physiology; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D003511:Cyclohexanols; D016207:Cytokines; D039821:Monoterpenes; D010446:Peptide Fragments; D017382:Reactive Oxygen Species; C074636:amyloid beta-protein (25-35); D000077591:Eucalyptol