title: Phage selection of peptide "microantibodies".
abstract: A bioactive peptide capable of inhibiting protein-protein interactions has the potential to be a molecular tool for biological studies and a therapeutic by disrupting aberrant interactions involved in diseases. We have developed combinatorial libraries of peptides with helix-loop-helix structure, from which the isolated peptides have the constrained structure to reduce entropy costs in binding, resulting in high binding affinities for target molecules. Previously, we designed a de novo peptide of helix-loop-helix structure that we termed a "microantibody." Using the microantibody as a library scaffold, we have constructed a phage-display library to successfully isolate molecular-targeting peptides against a cytokine receptor (granulocyte colony-stimulating factor receptor), a protein kinase (Aurora-A), and a ganglioside (GM1). Protocols in this article describe a general procedure for the library construction and the library screening.
authors.affiliation: Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Osaka, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Fujiwara
authors.forename: Ikuo
authors.initials: I
authors.lastname: Fujii
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology*; D001435:Bacteriophages / Q000276:immunology*; D004926:Escherichia coli / Q000821:virology; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D019151:Peptide Library; D010455:Peptides / Q000276:immunology*; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000737:chemistry / Q000276:immunology
chemical_list: D000906:Antibodies; D019151:Peptide Library; D010455:Peptides; D014764:Viral Proteins
keywords: microantibody; peptide; phage display; protein-protein interaction