title: A mouse air pouch model for evaluating the immune response to Taenia crassiceps infection.
abstract: The experimental system of Taenia crassiceps cysticerci infection in BALB/c mice is considered to be the most representative model of cysticercosis. In our work, mice were sacrificed 7 and 30days after infection, and pouch fluid was collected to determine the number of accumulated cells and the concentrations of IFNγ, IL-2, IL-4, IL-6, IL-10 and nitric oxide. The injection of 50 nonbudding cysticerci into normal mouse dorsal air pouches induced a high level of IFNγ and nitric oxide production relative to the parasite load. The air pouch provides a convenient cavity that allows studying the cellular immunological aspects of the T. crassiceps parasite. The nonbudding cysticerci recovered from the air pouches contained cells that can reconstitute complete cysts in the peritoneal cavity of mice. In conclusion, these results demonstrate that the air pouch model is an alternative tool for the evaluation of the immune characteristics of T. crassiceps infection.
authors.affiliation: Embrapa South Animal Husbandry & Sheep, Bagé, RS, Brazil.
authors.forename: Emanuelle B
authors.initials: EB
authors.lastname: Gaspar
authors.affiliation: Department of Pathology, Adolfo Lutz Institute, São Paulo, SP, Brazil.
authors.forename: Yuriko I
authors.initials: YI
authors.lastname: Sakai
authors.affiliation: Department of Immunology, Adolfo Lutz Institute, São Paulo, SP, Brazil. Electronic address: egaspari@ial.sp.gov.br.
authors.forename: Elizabeth De
authors.initials: ED
authors.lastname: Gaspari
mesh_terms: D000818:Animals; D015194:Diffusion Chambers, Culture; D004195:Disease Models, Animal; D005122:Exudates and Transudates / Q000166:cytology / Q000276:immunology; D005260:Female; D007371:Interferon-gamma / Q000032:analysis; D007378:Interleukins / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D010529:Peritoneal Cavity / Q000469:parasitology; D013621:Taenia / Q000276:immunology*; D013622:Taeniasis / Q000276:immunology*
chemical_list: D007378:Interleukins; D009569:Nitric Oxide; D007371:Interferon-gamma
keywords: Cysticercosis; Cytokines and nitric oxide; Dorsal air pouches; IL; Immunological response; NO; OD; Taenia crassiceps; Taenia crassiceps cysticerci; Taenia crassiceps vesicular fluid; VF-Tcra; i.p.; interleukin; intraperitoneal immunization; nitric oxide; optical density; s.c.; subcutaneous immunization