title: The polyphenol extract from Sechium edule shoots inhibits lipogenesis and stimulates lipolysis via activation of AMPK signals in HepG2 cells.
abstract: Fatty liver may have implications for metabolic syndrome, such as obesity, hypertension, and diabetes. Therefore, the development of pharmacological or natural agents to reduce fat accumulation in the liver is an important effort. The Sechium edule shoots have already been verified to decrease serum lipids and cholesterol and prevent atherosclerosis. However, how Sechium edule shoots modulate hepatic lipid metabolism is unclear. This study was designed to investigate the effects and mechanisms of polyphenol extracts (SPE) of Sechium edule shoots in reducing lipid accumulation in oleic acid-treated HepG2 cells. We found that water extracts (SWE) of Sechium edule shoots could decrease serum and hepatic lipid contents (e.g., triacylglycerol and cholesterol). Furthermore, SWE and SPE through the AMPK (AMP-activating protein kinase) signaling pathway could decrease lipogenic relative enzymes, such as FAS (fatty acid synthase), HMGCoR (HMG-CoA reductase), and SREBPs (sterol regulatory element binding proteins), and increase the expression of CPT-I (carnitine palmitoyltransferase I) and PPARα (peroxisome proliferators activated receptor α), which are critical regulators of hepatic lipid metabolism. These observations suggested that Sechium edule shoots have potential for developing health foods for preventing and remedying fatty liver.
authors.affiliation: Department of Anatomy, China Medical University , Taichung 40402, Taiwan.
authors.forename: Cheng-Hsun
authors.initials: CH
authors.lastname: Wu
authors.forename: Ting-Tsz
authors.initials: TT
authors.lastname: Ou
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Chang
authors.forename: Xiao-Zong
authors.initials: XZ
authors.lastname: Chang
authors.forename: Mon-Yuan
authors.initials: MY
authors.lastname: Yang
authors.forename: Chau-Jong
authors.initials: CJ
authors.lastname: Wang
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000214:Acyl Coenzyme A / Q000378:metabolism; D019662:Cucurbitaceae / Q000737:chemistry*; D005234:Fatty Liver / Q000188:drug therapy / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects; D050155:Lipogenesis / Q000187:drug effects*; D008066:Lipolysis / Q000187:drug effects*; D047493:PPAR alpha / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D018520:Plant Shoots / Q000737:chemistry*; D059808:Polyphenols / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000378:metabolism
chemical_list: D000214:Acyl Coenzyme A; D047493:PPAR alpha; D010936:Plant Extracts; D059808:Polyphenols; D051780:Sterol Regulatory Element Binding Protein 1; C008047:3-hydroxy-3-methylglutaryl-coenzyme A; D055372:AMP-Activated Protein Kinases
keywords: AMPK; FAS; HMGCoR; SPE (polyphenol extracts of Sechium edule shoots); SREBPs; SWE (water extracts of Sechium edule shoots)