title: The phenolics from the roots of Livistona chinensis show antioxidative and obsteoblast differentiation promoting activity.
abstract: This study investigated the antioxidative and obsteoblast differentiation promoting activity of the phenolics isolated from the 70% ethanol extract of the roots of Livistona chinensis. Two new phenolics, (2R,3R)-3,5,6,7,3',4'-hexahydroxyflavane (1), and phenanthrene-2,4,9-triol (2), together with six known phenolics 3-8, were isolated and identified on the basis of extensive spectroscopic analysis. The antioxidative and obsteoblast differentiation promoting abilities of the compounds 1-3, 7-8 were tested, the phenolics 1-3, 7 showed effects on proliferation of osteoblastic cells and antioxidative activity of 3.125-50 µg/mL. In addition, the phenolics 1-3 observably increased alkaline phosphatase activity, osteocalcin content and hydroxyproline content in osteoblastic cells. Phenolic 1 at 12.5 µg/mL concentration significantly increased the area of nodules by about 9.35-fold. The antioxidative activity results indicated that the anti-osteoporosis effects of these phenolics may be linked to a reduction of oxidative stress. The observed effects of these phenolics on bone formation by rat osteoblastic cells suggest that these phenolics may have beneficial effects on bone health.
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, Guangdong, China. 214210597@163.com.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, Guangdong, China. tj-085@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Tian
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, Guangdong, China. cuiliao@163.com.
authors.forename: Liao
authors.initials: L
authors.lastname: Cui
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, Guangdong, China. cherryyaya@fomail.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, Guangdong, China. super126su@126.com.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Su
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, Guangdong, China. 18820648019@163.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drugs, Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, Guangdong, China. hexiangjiu@whu.edu.cn.
authors.forename: Xiangjiu
authors.initials: X
authors.lastname: He
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D028023:Arecaceae / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D032446:Myoblasts / Q000187:drug effects / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects*; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D010636:Phenols / Q000737:chemistry* / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010636:Phenols; D015675:Osteocalcin; D003094:Collagen; D000469:Alkaline Phosphatase