title: Genotypic characterization of Chilean llama (Lama glama) and alpaca (Vicugna pacos) pestivirus isolates.
abstract: Llamas and alpacas are domesticated South American camelids (SACs) important to ancestral population in the Altiplano region, and to different communities worldwide where they have been introduced. These ungulates have shown to be susceptible to several livestock viral pathogens such as members of the Pestivirus genus, in particular Bovine Viral Diarrhea (BVDV), but there is little data available on Pestivirus infections in SACs. In this study we aimed to detect and identify Pestivirus genotypes and subgroups infecting SACs in both wild and confined environments. Samples were collected from 136 llamas and 30 alpacas from different areas in the Chilean Altiplano (wild animals), and from 22 llamas and 26 alpacas diagnosed as Pestivirus positive from the Metropolitana region in Chile (confined animals). Seroneutralization tests showed titers lower than 2 in all 166 samples from Chilean Altiplano. These samples were also negative to BVDV isolation, indicating that these animals have not been exposed to Pestivirus. After reactivation of positive samples from the Metropolitana region, the 5' non-codifying region (5'NCR) and E2 glycoprotein were amplified by RT-PCR from the Pestivirus genome. Viral sequences were pairwise compared and phylogenetic trees were constructed. The 5'NCR analysis showed that all 12 sequenced isolates belonged to BVDV-1. Of particular interest, isolates from eight llama and two alpaca were BVDV-1j and two alpacas were BVDV-1b. In agreement with these results, E2 phylogenetic analysis rendered a similar grouping indicating that all 16 isolates belong to BVDV-1. However, the lower availability of E2 sequences determines the creation of a smaller number of sub-groups than the 5'NCR sequences. Based on the E2 sequences, the 5'NCR BVDV 1j group consisting of all the llamas and 3 alpacas are completely included in the E2 BVDV 1e group. Due to the universal availability of the 5'NCR segment, we propose the classification of these Chilean llamas and alpacas Pestivirus isolates as BVDV 1j and BVDV 1b respectively. Thus, this is the first time BVDV-1j is obtained in SACs. In addition, these results indicate Pestivirus infection in llamas and alpacas is associated with bovine population as genotypes and sub-groups are the same as those affecting Chilean livestock.
authors.affiliation: Universidad de Chile, Facultad de Ciencias Veterinarias y Pecuarias, Departamento de Medicina Preventiva, Laboratorio de Virología, Av. Santa Rosa 11735, La Pintana, Santiago, Chile. Electronic address: iaguirregil@gmail.com.
authors.forename: I M
authors.initials: IM
authors.lastname: Aguirre
authors.affiliation: Universidad de Chile, Facultad de Ciencias Veterinarias y Pecuarias, Departamento de Medicina Preventiva, Laboratorio de Virología, Av. Santa Rosa 11735, La Pintana, Santiago, Chile.
authors.forename: R
authors.initials: R
authors.lastname: Fuentes
authors.affiliation: Universidad de Chile, Facultad de Ciencias Veterinarias y Pecuarias, Departamento de Medicina Preventiva, Laboratorio de Virología, Av. Santa Rosa 11735, La Pintana, Santiago, Chile.
authors.forename: M O
authors.initials: MO
authors.lastname: Celedón
mesh_terms: D000818:Animals; D002161:Camelids, New World / Q000821:virology*; D002677:Chile; D030241:Diarrhea Virus 1, Bovine Viral / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005838:Genotype; D018182:Pestivirus Infections / Q000235:genetics / Q000662:veterinary* / Q000821:virology*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction
keywords: BVDV; Phylogenetic analysis; South American camelids