title: Use of granulocyte colony-stimulating factor for the treatment of thin endometrium in experimental rats.
abstract: Granulocyte colony-stimulating factor (G-CSF) induces stem cells to mobilize to the injury site, which have beneficial effect on tissue repair. The aim of this study was to investigate the effect of G-CSF on the thin endometrium in rat models. In the present study, rats with thin endometrium were divided into 4 groups (experimental group I: administrated with G-CSF (40 µg/kg/d) 4-6 hours post-modeling; control group I: administrated with saline 4-6 hours post-modeling; experimental group II: administrated with G-CSF (40 µg/kg/d) 12 days post-modeling; control group II: administrated with saline 12 days post-modeling. The agentia was given once daily and last for 5 days. Endometrial morphology was analyzed by Hematoxylin-Eosin staining, and the regeneration of endometrial cells was evaluated by immunohistochemistry and western-blot with cytokeratin and vimentin. We found that endometrial thickness and morphology presented a significant difference between experimental groups and control groups. No matter when we start with G-CSF, there was a significantly thicker endometrium and stronger expression of cytokeratin/vimintin in the experimental groups compared with the control groups (P<0.01). There were significant thicker endometrial lining and stronger expression of cytokeratin/vimintin in experimental group I than that of experimental group II (P<0.05), but there was no difference in the endometrial lining and the expression of cytokeratin/vimintin between the two control groups (P>0.05). In conclusion, G-CSF can promote the regeneration of endometrial cells in animal research, especially when the G-CSF was administrated earlier.
authors.affiliation: Reproductive Medicine Center, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Reproductive Medicine Center, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.affiliation: Reproductive Medicine Center, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Reproductive Medicine Center, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Reproductive Medicine Center, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D004717:Endometrium / Q000187:drug effects* / Q000473:pathology*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D007150:Immunohistochemistry; D007633:Keratins / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000187:drug effects; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D014746:Vimentin / Q000378:metabolism
chemical_list: D014746:Vimentin; D016179:Granulocyte Colony-Stimulating Factor; D007633:Keratins