title: Dynamic intrinsic optical signal monitoring of electrically stimulated inner retinal neural response.
abstract: Dynamic monitoring of stimulus-evoked inner neural response is important for functional validation of stimulation protocols of retinal prosthetic devices. In this paper, we demonstrate label-free intrinsic optical signal (IOS) imaging of electrically stimulated inner neural response in freshly isolated mouse retinas. While single-pulse stimulation evoked rapid IOS within 20 ms, pulse-train stimulation indicated that the fast IOS response can follow frequency stimulation up to at least 8 Hz. Fast IOS imaging promises a noninvasive method for high resolution examination of electrically evoked retinal response, without artifact contamination of electrical stimulus.
authors.affiliation: Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
authors.forename: Yi-Chao
authors.initials: YC
authors.lastname: Li
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Nebraska Medical Center, Omaha, NE 68138, USA.
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Luo
authors.affiliation: Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
authors.forename: Rong-Wen
authors.initials: RW
authors.lastname: Lu
authors.affiliation: Department of Biochemistry & Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
authors.forename: Kai-Mao
authors.initials: KM
authors.lastname: Liu
authors.affiliation: Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
authors.forename: Alexander Miles
authors.initials: AM
authors.lastname: Levy
authors.affiliation: Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA ; Department of Vision Sciences, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
authors.forename: Xin-Cheng
authors.initials: XC
authors.lastname: Yao
keywords: ganglion cell; intrinsic optical signal; retinal function; retinal prosthesis