title: Gene expression changes after ionizing radiation in endothelial cells derived from human endometrial cancer-preliminary outcomes.
abstract: BACKGROUND
  Accumulating evidence has demonstrated that death of microvascular endothelial cells plays a decisive role in the tumor response against radiotherapy. Nevertheless, radiation-induced gene alterations on cancer-associated endothelial cells of human endometrial carcinoma remain poorly understood. The purpose of this study was to elucidate the gene expression changes after X-ray radiation in human endometrial carcinoma vascular endothelial cells and to provide new targets for combined treatment of radiation and anti-angiogenesis in human endometrial carcinoma.
  
  
  METHODS
  Endometrial cancer-derived endothelial cells, which obtained before and 4 h after 400 cGy X-ray radiation from four endometrial carcinomas, were analyzed by gene expression profile. The selected meaningful genes from gene microarray experiments were validated by real-time quantitative PCR.
  
  
  RESULTS
  Microarray analyses showed 49 significantly changed genes which were common to all the microarray experiments. There into, 14 genes were found to be in persistent up-regulation and 14 in persistent down-regulation 4 h after X-ray radiation when compared with the control group. These genes were involved in cell cycle and growth regulation, cell-apoptosis, chemokine, cell signaling, cellular stress response, angiogenesis, DNA synthesis and repair and cell adhesion. Eight randomly selected genes were validated by real-time PCR.
  
  
  CONCLUSIONS
  The genes of cancer-derived endothelial cells regulated by X-ray radiation as well as their related signal pathways, which obtained from gene expression profiling data, were relevant to radiosensitivity of endometrial cancer. This study shows that the identified genes and their related signaling pathways are candidated biomarkers for radiation and anti-angiogenesis of human endometrial carcinoma.
authors.affiliation: Department of Gynecologic Oncology, Shandong Cancer Hospital, Jinan, 250117, Shandong, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.forename: Xuelian
authors.initials: X
authors.lastname: Du
authors.forename: Xiugui
authors.initials: X
authors.lastname: Sheng
mesh_terms: D000328:Adult; D002277:Carcinoma / Q000473:pathology / Q000532:radiotherapy*; D002478:Cells, Cultured; D015536:Down-Regulation; D016889:Endometrial Neoplasms / Q000473:pathology / Q000532:radiotherapy*; D042783:Endothelial Cells / Q000473:pathology / Q000528:radiation effects*; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D015870:Gene Expression / Q000528:radiation effects*; D020869:Gene Expression Profiling; D006801:Humans; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D011836:Radiation Tolerance / Q000235:genetics; D011879:Radiotherapy Dosage; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation