title: Monoclonal antibodies raised against 167-180 aa sequence of human carbonic anhydrase XII inhibit its enzymatic activity.
abstract: Abstract Human carbonic anhydrase XII (CA XII) is a single-pass transmembrane protein with an extracellular catalytic domain. This enzyme is being recognized as a potential biomarker for different tumours. The current study was aimed to generate monoclonal antibodies (MAbs) neutralizing the enzymatic activity of CA XII. Bioinformatics analysis of CA XII structure revealed surface-exposed sequences located in a proximity of its catalytic centre. Two MAbs against the selected antigenic peptide spanning 167-180 aa sequence of CA XII were generated. The MAbs were reactive with recombinant catalytic domain of CA XII expressed either in E. coli or mammalian cells. Inhibitory activity of the MAbs was demonstrated by a stopped flow CO2 hydration assay. The study provides new data on the surface-exposed linear CA XII epitope that may serve as a target for inhibitory antibodies with a potential immunotherapeutic application.
authors.affiliation: Institute of Biotechnology, Vilnius University , Vilnius , Lithuania .
authors.forename: Dovile
authors.initials: D
authors.lastname: Dekaminaviciute
authors.forename: Visvaldas
authors.initials: V
authors.lastname: Kairys
authors.forename: Milda
authors.initials: M
authors.lastname: Zilnyte
authors.forename: Vilma
authors.initials: V
authors.lastname: Petrikaite
authors.forename: Vaida
authors.initials: V
authors.lastname: Jogaite
authors.forename: Jurgita
authors.initials: J
authors.lastname: Matuliene
authors.forename: Zivile
authors.initials: Z
authors.lastname: Gudleviciene
authors.forename: Daniela
authors.initials: D
authors.lastname: Vullo
authors.forename: Claudiu T
authors.initials: CT
authors.lastname: Supuran
authors.forename: Aurelija
authors.initials: A
authors.lastname: Zvirbliene
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D002257:Carbonic Anhydrase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D019295:Computational Biology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006433:Hemocyanins / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000737:chemistry / Q000276:immunology; D007114:Immunization; D018796:Immunoconjugates / Q000008:administration & dosage / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D002257:Carbonic Anhydrase Inhibitors; D000939:Epitopes; D018796:Immunoconjugates; D009842:Oligopeptides; D011994:Recombinant Proteins; D006433:Hemocyanins; D002256:Carbonic Anhydrases; C477378:carbonic anhydrase XII; C032808:keyhole-limpet hemocyanin
keywords: Human carbonic anhydrase XII; inhibition of enzymatic activity; monoclonal antibodies