title: Bovine lactoferrin purification from whey using Yellow HE-4R as the chromatographic affinity ligand.
abstract: The worldwide production of whey increases by around 186 million tons each year and it is generally considered as a waste, even when several whey proteins have important economic relevance. For its valorization, inexpensive ligands and integrated chromatography methods need to be developed for specific and low-cost protein purification. Here, we describe a novel affinity process with the dye Yellow HE-4R immobilized on Sepharose for bovine lactoferrin purification. This approach based on a low-cost ligand showed an efficient performance for the recovery and purification of bovine lactoferrin directly from whey, with a yield of 71% and a purification factor of 61.
authors.affiliation: Cátedra de Microbiología Industrial y Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, Argentina.
authors.forename: María Fernanda
authors.initials: MF
authors.lastname: Baieli
authors.forename: Nicolás
authors.initials: N
authors.lastname: Urtasun
authors.forename: María Victoria
authors.initials: MV
authors.lastname: Miranda
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Cascone
authors.forename: Federico Javier
authors.initials: FJ
authors.lastname: Wolman
mesh_terms: D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry; D007781:Lactoferrin / Q000737:chemistry / Q000302:isolation & purification*; D008024:Ligands; D008894:Milk Proteins / Q000737:chemistry / Q000302:isolation & purification*; D000067816:Whey Proteins
chemical_list: D004396:Coloring Agents; D008024:Ligands; D008894:Milk Proteins; D000067816:Whey Proteins; D007781:Lactoferrin
keywords: Bovine lactoferrin; Dye affinity chromatography; Sweet whey; Triazine dyes