title: Antitumor effect and mechanism of an ellagic acid derivative on the HepG2 human hepatocellular carcinoma cell line.
abstract: In the present study, to identify the effective components of Chinese traditional herbs,Euphorbia hylonomaHand.-Mazz. (Euphorbiaceae), a folk herb that has been used among the Qinling mountain area for hundreds of years, was investigated. 3,3'-Di-O-methyl ellagic acid-4'-O-β-d-xylopyranoside (JNE2), an ellagic acid derivative, was isolated from the acetone extract of the herb and its antitumor activity against human hepatoma HepG2 cells was detectedin vitro. The results showed that JNE2 inhibited the proliferation of HepG2 cells in a dose- and time-dependent manner and blocked the cell cycle at the G1/S phase. A high dosage of JNE2 induced apoptosis of the tumor cells, but no significant differences were identified between the treatment groups. The invasiveness of HepG2 cells was also inhibited by JNE2. The mechanism of the antitumor effect of JNE2 at the molecular level was presumed to be due to the upregulation of the protein expression of Bax and caspase-3, and the downregulation of the protein expression of Bcl-2 and CCND1. The results suggested that JNE2 is a potential antitumor agent that merits further investigation.
authors.affiliation: Faculty of Pharmacy, Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Faculty of Pharmacy, Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Zeng-Jun
authors.initials: ZJ
authors.lastname: Guo
authors.affiliation: Faculty of Pharmacy, Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Xu
authors.affiliation: Faculty of Pharmacy, Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: You
authors.affiliation: Faculty of Pharmacy, Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Han
authors.affiliation: Faculty of Pharmacy, Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Yan-Xia
authors.initials: YX
authors.lastname: Han
authors.affiliation: Faculty of Pharmacy, Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Liu-Jiang
authors.initials: LJ
authors.lastname: Dai
keywords: Euphorbia hylonoma Hand.-Mazz.; antitumor activity; ellagic acid derivative; human hepatoma HepG2