title: Downregulation of CRKL expression can inhibit tumorigenesis in colon cancer.
abstract: CRKL, as a "switch" factor on several oncogenic pathways, plays vital roles in multiple cancers. However, little is known about CRKL in gastrointestinal cancers. Here, we showed that CRKL is involved in colon cancer, which is the most common form of cancer of the digestive system. Immunohistochemistry analysis showed that CRKL expression in colon tumor tissue is significantly higher than normal tissue and CRKL level is associated with tumor differentiation. Suppression of CRKL in colon cancer cells inhibited cell proliferation, migration and invasion, while induced apoptosis. Colon cancer cells xenografts in nude mice showed that CRKL promoted tumorigenesis. Our results suggest that CRKL has the ability to regulate colon cancer malignancy and CRKL has the potential to serve as a diagnosis and prognosis marker and a therapy target of colon cancer.
authors.affiliation: Department of gastroenterology, the First Affiliated Hospital, Fujian Medical University, 20 Chazhong Rd, Fuzhou 350005, Fujian Province, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Lan
authors.affiliation: Department of Neurology, the Affiliated Union Hospital, Fujian Medical University, 29 Xinquan Rd, Fuzhou 350001, Fujian Province, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Laboratory of Genetic Medicine and Immunology, Weill Cornell Medical College in Qatar - Qatar Foundation, Education City, P.O.Box 24144, Doha Qatar.
authors.forename: Jingxuan
authors.initials: J
authors.lastname: Shan
authors.affiliation: Department of Thoracic Surgery, Shanghai Pulmonary Hospital of Tongji University,507 Zhengmin Rd., Shanghai 200433 PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of gastroenterology, the First Affiliated Hospital, Fujian Medical University, 20 Chazhong Rd, Fuzhou 350005, Fujian Province, China.
authors.forename: Weihong
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of gastroenterology, the First Affiliated Hospital, Fujian Medical University, 20 Chazhong Rd, Fuzhou 350005, Fujian Province, China.
authors.forename: Yanchao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of gastroenterology, the First Affiliated Hospital, Fujian Medical University, 20 Chazhong Rd, Fuzhou 350005, Fujian Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhen
authors.affiliation: Department of gastroenterology, the First Affiliated Hospital, Fujian Medical University, 20 Chazhong Rd, Fuzhou 350005, Fujian Province, China.
authors.forename: Shugang
authors.initials: S
authors.lastname: Yan
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000502:physiology*; D000818:Animals; D015153:Blotting, Western; D063646:Carcinogenesis; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C082869:CRKL protein; D009687:Nuclear Proteins