title: Treadmill exercise preconditioning attenuates lung damage caused by systemic endotoxemia in type 1 diabetic rats.
abstract: Endotoxemia induces a series of inflammatory responses that may result in lung injury. However, heat shock protein72 (HSP72) has the potential to protect the lungs from damage. The objective of this study was to determine whether prior exercise conditioning could increase the expression of HSP72 in the lungs and attenuate lung damage in diabetic rats receiving lipopolysaccharide (LPS). Streptozotocin was used to induce diabetes in adult male Wistar rats. Rats were randomly assigned to sedentary or exercise groups. Rats in the exercise condition ran on a treadmill 5 days/week, 30-60 min/day, with an intensity of 1.0 mile/hour over a 3-week period. Rats received an intravenous infusion of LPS after 24 hrs from the last training session. Elevated lavage tumor necrosis factor-alpha (TNF- α ) level in response to LPS was more marked in diabetic rats. HSP72 expression in lungs was significantly increased after exercise conditioning, but less pronounced in diabetic rats. After administration of LPS, exercised rats displayed higher survival rate as well as decreased lavage TNF- α level and lung edema in comparison to sedentary rats. Our findings suggest that exercise conditioning could attenuate the occurrence of inflammatory responses and lung damage, thereby reducing mortality rate in diabetic rats during endotoxemia.
authors.affiliation: Department of Physical Therapy, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Ching-Hsia
authors.initials: CH
authors.lastname: Hung
authors.affiliation: Department of Food Sciences and Technology, Chia Nan University of Pharmacy and Sciences, Jen-Te, Tainan 717, Taiwan ; Department of Anesthesiology, Chi-Mei Medical Center, Yong Kang, Tainan 710, Taiwan.
authors.forename: Jann-Inn
authors.initials: JI
authors.lastname: Tzeng
authors.affiliation: Department of Physical Therapy, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Che-Ning
authors.initials: CN
authors.lastname: Chang
authors.affiliation: Department of Physical Therapy, China Medical University, Taichung 404, Taiwan.
authors.forename: Yu-Wen
authors.initials: YW
authors.lastname: Chen
authors.affiliation: Department of Physical Therapy, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Chia-Ying
authors.initials: CY
authors.lastname: Cho
authors.affiliation: Department of Medical Research, Chi-Mei Medical Centre, Tainan 710, Taiwan.
authors.forename: Jhi-Joung
authors.initials: JJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000628:therapy; D003922:Diabetes Mellitus, Type 1 / Q000139:chemically induced / Q000150:complications* / Q000628:therapy*; D019446:Endotoxemia / Q000150:complications* / Q000628:therapy*; D005080:Exercise Test; D050884:HSP72 Heat-Shock Proteins / Q000378:metabolism; D008070:Lipopolysaccharides; D008168:Lung / Q000378:metabolism; D008171:Lung Diseases / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D010805:Physical Conditioning, Animal / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D050884:HSP72 Heat-Shock Proteins; D008070:Lipopolysaccharides