title: High passage numbers induce resistance to apoptosis in C2C12 muscle cells.
abstract: Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (< 20) or high (> 60) passage numbers (identified as 1-C2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2O2 induction of apoptosis. We propose serially cultured C2Cl2 cells as a refractory model to H2O2-induced apoptosis. In addition, the data obtained in this work suggest that mtDNA is required for apoptotic cell death in skeletal muscle C2C12 cells.
authors.affiliation: Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur. San Juan 670, 8000 Bahía Blanca, Argentina.
authors.forename: Lucia
authors.initials: L
authors.lastname: Pronsato
authors.affiliation: Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur. San Juan 670, 8000 Bahía Blanca, Argentina.
authors.forename: Anabela
authors.initials: A
authors.lastname: La Colla
authors.affiliation: Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur. San Juan 670, 8000 Bahía Blanca, Argentina.
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Ronda
authors.affiliation: Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur. San Juan 670, 8000 Bahía Blanca, Argentina.
authors.forename: Lorena
authors.initials: L
authors.lastname: Milanesi
authors.affiliation: Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur. San Juan 670, 8000 Bahía Blanca, Argentina.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Boland
authors.affiliation: Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur. San Juan 670, 8000 Bahía Blanca, Argentina.
authors.forename: Andrea
authors.initials: A
authors.lastname: Vasconsuelo
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D018929:Cell Culture Techniques; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D047468:Immunoprecipitation; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D032448:Myoblasts, Skeletal / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D016877:Oxidants / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494827:Bax protein, mouse; D016877:Oxidants; D051028:bcl-2-Associated X Protein; D006861:Hydrogen Peroxide; C505409:Casp3 protein, mouse; D053148:Caspase 3