title: Establishing a core microbiome in acetate-fed microbial fuel cells.
abstract: Establishing a core microbiome is the first step in understanding and subsequently optimizing microbial interactions in anodic biofilms of microbial fuel cells (MFCs) for increased power, efficiency, and decreased start-up times. In the present study, we used 454 pyrosequencing to demonstrate that a core anodic community would consistently emerge over a period of 4 years given similar conditions. The development and variation across reactor designs of these communities was also explored. The core members present in all high-power generating biofilms were Geobacter, Aminiphilus, Sedimentibacter, Acetoanaerobium, and Spirochaeta, accounting for 72 ± 9 % of all genera. Aminiphilus spp., member of the Synergistetes phylum was present at higher abundances than previously reported in any other ecological studies. Results suggest a stable core microbiome in acetate-fed MFCs on both phylogenetic and functional levels.
authors.affiliation: Department of Biological and Ecological Engineering, Oregon State University, Corvallis, OR, 97333, USA.
authors.forename: Keaton Larson
authors.initials: KL
authors.lastname: Lesnik
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
mesh_terms: D000085:Acetates / Q000378:metabolism*; D001419:Bacteria / Q000145:classification*; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004566:Electrodes / Q000382:microbiology; D064307:Microbiota; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D000085:Acetates; D004269:DNA, Bacterial