title: GsLRPK, a novel cold-activated leucine-rich repeat receptor-like protein kinase from Glycine soja, is a positive regulator to cold stress tolerance.
abstract: Plant LRR-RLKs serve as protein interaction platforms, and as regulatory modules of protein activation. Here, we report the isolation of a novel plant-specific LRR-RLK from Glycine soja (termed GsLRPK) by differential screening. GsLRPK expression was cold-inducible and shows Ser/Thr protein kinase activity. Subcellular localization studies using GFP fusion protein indicated that GsLRPK is localized in the plasma membrane. Real-time PCR analysis indicated that temperature, salt, drought, and ABA treatment can alter GsLRPK gene transcription in G. soja. However, just protein induced by cold stress not by salinity and ABA treatment in tobacco was found to possess kinase activity. Furthermore, we found that overexpression of GsLRPK in yeast and Arabidopsis can enhance resistance to cold stress and increase the expression of a number of cold responsive gene markers.
authors.affiliation: Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agricultural and Forestry University, Fuzhou 350002, Fujian, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agricultural and Forestry University, Fuzhou 350002, Fujian, China.
authors.forename: Kangcheng
authors.initials: K
authors.lastname: Wu
authors.affiliation: College of Horticulture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.affiliation: Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agricultural and Forestry University, Fuzhou 350002, Fujian, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
authors.forename: Guangpu
authors.initials: G
authors.lastname: Li
authors.affiliation: Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agricultural and Forestry University, Fuzhou 350002, Fujian, China. Electronic address: wuzujian@126.com.
authors.forename: Zujian
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000222:Adaptation, Physiological / Q000235:genetics*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003080:Cold Temperature; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007930:Leucine / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D055049:Salt Tolerance / Q000235:genetics; D030262:Soybean Proteins / Q000235:genetics* / Q000378:metabolism; D013025:Glycine max / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D030262:Soybean Proteins; D000040:Abscisic Acid; D011494:Protein Kinases; D007930:Leucine
keywords: Abiotic stress; Cold tolerance; Leucine-rich repeat receptor-like protein kinase (LRR-RLK); Wild soybean