title: Cross-species protection: Schistosoma mansoni Sm-p80 vaccine confers protection against Schistosoma haematobium in hamsters and baboons.
abstract: The ability of the Schistosoma mansoni antigen, Sm-p80, to provide cross-species protection against Schistosoma haematobium challenge was evaluated in hamster and baboon models. Pronounced reduction in worm burden (48%) and in tissue egg load (64%) was observed in hamsters vaccinated with recombinant Sm-p80 admixed with glucopyranosyl lipid adjuvant-stable emulsion (GLA-SE). Similarly, in baboons, the Sm-p80/GLA-SE vaccine produced a 25% reduction in S. haematobium adult worms and decreased the egg load in the urinary bladder by 64%. A 40% and 53% reduction in fecal and urine egg output, respectively, was observed in vaccinated baboons. A balanced pro-inflammatory (Th17 and Th1) and Th2 type of response was generated after vaccination and appears indicative of augmented prophylactic efficacy. These data on cross-species protection coupled with the prophylactic, therapeutic and antifecundity efficacy against the homologous parasite, S. mansoni, reinforces Sm-p80 as a promising vaccine candidate. It is currently being prepared for GMP-compliant manufacture and for further pre-clinical development leading to human clinical trials. These results solidify the expectation that the Sm-p80 vaccine will provide relief for both the intestinal and the urinary schistosomiasis and thus will be greatly beneficial in reducing the overall burden of schistosomiasis.
authors.affiliation: Center for Tropical Medicine and Infectious Diseases, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA; Department of Immunology and Molecular Microbiology, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA.
authors.forename: Souvik
authors.initials: S
authors.lastname: Karmakar
authors.affiliation: Center for Tropical Medicine and Infectious Diseases, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA; Department of Immunology and Molecular Microbiology, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA.
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Natural Sciences, School of Arts & Sciences, Peru State College, Peru, NE 68321, USA.
authors.forename: Gul
authors.initials: G
authors.lastname: Ahmad
authors.affiliation: Tulane National Primate Research Center, Covington, LA 70433, USA.
authors.forename: Workineh
authors.initials: W
authors.lastname: Torben
authors.affiliation: Center for Tropical Medicine and Infectious Diseases, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA; Department of Immunology and Molecular Microbiology, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA.
authors.forename: Mayeen U
authors.initials: MU
authors.lastname: Alam
authors.affiliation: Center for Tropical Medicine and Infectious Diseases, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA; Department of Immunology and Molecular Microbiology, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA.
authors.forename: Loc
authors.initials: L
authors.lastname: Le
authors.affiliation: Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA.
authors.forename: Raymond T
authors.initials: RT
authors.lastname: Damian
authors.affiliation: Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
authors.forename: Roman F
authors.initials: RF
authors.lastname: Wolf
authors.affiliation: Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
authors.forename: Gary L
authors.initials: GL
authors.lastname: White
authors.affiliation: Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
authors.forename: David W
authors.initials: DW
authors.lastname: Carey
authors.affiliation: Infectious Disease Research Institute, Seattle, WA 98102, USA; PAI Life Sciences, Seattle, WA 98102, USA.
authors.forename: Darrick
authors.initials: D
authors.lastname: Carter
authors.affiliation: Infectious Disease Research Institute, Seattle, WA 98102, USA.
authors.forename: Steven G
authors.initials: SG
authors.lastname: Reed
authors.affiliation: Center for Tropical Medicine and Infectious Diseases, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA; Department of Immunology and Molecular Microbiology, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA; Department of Internal Medicine, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA; Department of Pathology, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA. Electronic address: Afzal.Siddiqui@ttuhsc.edu.
authors.forename: Afzal A
authors.initials: AA
authors.lastname: Siddiqui
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000917:Antibody Formation; D000947:Antigens, Helminth / Q000276:immunology; D006224:Cricetinae; D056738:Cross Protection; D016207:Cytokines / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D007111:Immunity, Cellular; D008297:Male; D010215:Papio; D010270:Parasite Egg Count; D010865:Pilot Projects; D012548:Schistosoma haematobium / Q000472:pathogenicity*; D012550:Schistosoma mansoni / Q000276:immunology*; D012552:Schistosomiasis / Q000517:prevention & control*; D014612:Vaccines / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D016207:Cytokines; D014612:Vaccines; D014614:Vaccines, Synthetic
keywords: Baboons; Calpain; Intestinal schistosomiasis; Nonhuman primate; Schistosoma haematobium; Schistosoma mansoni; Schistosome vaccine; Sm-p80; Urinary schistosomiasis