title: Antimicrobial properties of an oxidizer produced by Burkholderia cenocepacia P525.
abstract: A compound with both oxidizing properties and antibiotic properties was extracted and purified from broth cultures of Burkholderia cenocepacia strain P525. A four step purification procedure was used to increase its specific activity ~400-fold and to yield a HPLC-UV chromatogram containing a single major peak. Size exclusion chromatography suggests a molecular mass of ~1,150 and UV spectroscopy suggests the presence of a polyene structure consisting of as many as six conjugated double bonds. Biological studies indicate that the compound is bacteriostatic. Enterobacter soli and E. aerogenes cells incubated with the compound exhibit a longer lag phase of growth. The bacteriostatic activity is greater at pH 3 than at pH 5. Bacteria such as B. cenocepacia strain P525 may have value in the agricultural industry as biocontrol agents.
authors.affiliation: Soil-Plant-Nutrient Research Unit, USDA-ARS, Fort Collins, CO, 80526-8119, USA, william.hunter@ars.usda.gov.
authors.forename: William J
authors.initials: WJ
authors.lastname: Hunter
authors.forename: Dan K
authors.initials: DK
authors.lastname: Manter
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D057508:Burkholderia cenocepacia / Q000378:metabolism*; D002850:Chromatography, Gel; D004754:Enterobacter / Q000187:drug effects* / Q000254:growth & development; D050296:Microbial Viability / Q000187:drug effects; D008970:Molecular Weight; D016877:Oxidants / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000890:Anti-Infective Agents; D016877:Oxidants