title: Ultrasensitive resonance scattering (RS) spectral detection for trace tetracycline in milk using aptamer-coated nanogold (ACNG) as a catalyst.
abstract: This paper reports an ultrasensitive resonance scattering (RS) method to detect tetracycline (TET) in milk based on the competition of aptamers between nanogold and TET, aggregation of naked nanogold, nanocatalytic Fehling reaction, and RS signals of catalytic product Cu₂O cubic. The detection principle was confirmed by the nanoparticle size analyzer (NANOS), scanning electron microscopy (SEM), and X-ray diffraction (XRD). The variations of RS intensity had good linear correlation with TET concentrations, and the limit of detection was calculated as 11.6 nM. The proposed method was successfully applied for analysis of TET in milk, with total recoveries ranging from 105 to 109%.
authors.affiliation: School of Agriculture and Biology, ‡Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Bor S. Luh Food Safety Research Center, and §School of Environmental Science and Engineering, Shanghai Jiao Tong University , Shanghai 200240, People's Republic of China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Luo
authors.forename: Lan
authors.initials: L
authors.lastname: He
authors.forename: Shenshan
authors.initials: S
authors.lastname: Zhan
authors.forename: Yuangen
authors.initials: Y
authors.lastname: Wu
authors.forename: Le
authors.initials: L
authors.lastname: Liu
authors.forename: Wenting
authors.initials: W
authors.lastname: Zhi
authors.forename: Pei
authors.initials: P
authors.lastname: Zhou
mesh_terms: D000327:Adsorption; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry; D002384:Catalysis; D002417:Cattle; D005506:Food Contamination / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013752:Tetracycline / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; D006046:Gold; D013752:Tetracycline