title: The study on biocompatibility of porous nHA/PLGA composite scaffolds for tissue engineering with rabbit chondrocytes in vitro.
abstract: OBJECTIVE
  To examine the biocompatibility of a novel nanohydroxyapatite/poly[lactic-co-glycolic acid] (nHA/PLGA) composite and evaluate its feasibility as a scaffold for cartilage tissue engineering.
  
  
  METHODS
  Chondrocytes of fetal rabbit were cultured with nHA/PLGA scaffold in vitro and the cell viability was assessed by MTT assay first. Cells adhering to nHA/PLGA scaffold were then observed by inverted microscope and scanning electron microscope (SEM). The cell cycle profile was analyzed by flow cytometry.
  
  
  RESULTS
  The viability of the chondrocytes on the scaffold was not affected by nHA/PLGA comparing with the control group as it was shown by MTT assay. Cells on the surface and in the pores of the scaffold increased in a time-dependent manner. Results obtained from flow cytometry showed that there was no significant difference in cell cycle profiles between the coculture group and control (P > 0.05).
  
  
  CONCLUSIONS
  The porous nHA/PLGA composite scaffold is a biocompatible and good kind of scaffold for cartilage tissue engineering.
authors.affiliation: Guangzhou Medical College, Guangzhou, Guangdong 510182, China ; Department of Orthopedics, Second People's Hospital of Shenzhen, Sungang West Road, Futian District, Shenzhen, Guangdong 518035, China ; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen, Guangdong 518035, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, Second People's Hospital of Shenzhen, Sungang West Road, Futian District, Shenzhen, Guangdong 518035, China ; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen, Guangdong 518035, China.
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Zhu
authors.affiliation: Department of Orthopedics, Second People's Hospital of Shenzhen, Sungang West Road, Futian District, Shenzhen, Guangdong 518035, China ; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen, Guangdong 518035, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Fei
authors.affiliation: Department of Orthopedics, Second People's Hospital of Shenzhen, Sungang West Road, Futian District, Shenzhen, Guangdong 518035, China ; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen, Guangdong 518035, China.
authors.forename: Jie-Lin
authors.initials: JL
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, Second People's Hospital of Shenzhen, Sungang West Road, Futian District, Shenzhen, Guangdong 518035, China ; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen, Guangdong 518035, China.
authors.forename: Jian-Yi
authors.initials: JY
authors.lastname: Xiong
authors.affiliation: Department of Orthopedics, Second People's Hospital of Shenzhen, Sungang West Road, Futian District, Shenzhen, Guangdong 518035, China ; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen, Guangdong 518035, China.
authors.forename: Ju-Feng
authors.initials: JF
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Second People's Hospital of Shenzhen, Sungang West Road, Futian District, Shenzhen, Guangdong 518035, China ; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen, Guangdong 518035, China.
authors.forename: Li
authors.initials: L
authors.lastname: Duan
authors.affiliation: Department of Orthopedics, Second People's Hospital of Shenzhen, Sungang West Road, Futian District, Shenzhen, Guangdong 518035, China ; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen, Guangdong 518035, China.
authors.forename: Jianghong
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Orthopedics, Second People's Hospital of Shenzhen, Sungang West Road, Futian District, Shenzhen, Guangdong 518035, China ; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen, Guangdong 518035, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Guangzhou Medical College, Guangzhou, Guangdong 510182, China ; Department of Orthopedics, Second People's Hospital of Shenzhen, Sungang West Road, Futian District, Shenzhen, Guangdong 518035, China ; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen, Guangdong 518035, China.
authors.forename: Daping
authors.initials: D
authors.lastname: Wang
authors.affiliation: Biomechanics and Medical Information Institute, Beijing University of Technology, Beijing 100022, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D019902:Chondrocytes / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry* / Q000494:pharmacology; D019344:Lactic Acid / Q000737:chemistry* / Q000494:pharmacology; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D017886:Durapatite