title: Generation of iPSC lines from archived non-cryoprotected biobanked dura mater.
abstract: BACKGROUND
  Induced pluripotent stem cells (iPSCs) derived from patients with neurodegenerative disease generally lack neuropathological confirmation, the gold standard for disease classification and grading of severity. The use of tissue with a definitive neuropathological diagnosis would be an ideal source for iPSCs. The challenge to this approach is that the majority of biobanked brain tissue was not meant for growing live cells, and thus was not frozen in the presence of cryoprotectants such as DMSO.
  
  
  RESULTS
  We report the generation of iPSCs from frozen non-cryoprotected dural tissue stored at -80°C for up to 11 years. This autopsy cohort included subjects with Alzheimer's disease and four other neurodegenerative diseases.
  
  
  CONCLUSIONS
  Disease-specific iPSCs can be generated from readily available, archival biobanked tissue. This allows for rapid expansion of generating iPSCs with confirmed pathology as well as allowing access to rare patient variants that have been banked.
authors.forename: Andrew A
authors.initials: AA
authors.lastname: Sproul
authors.forename: Lauren B
authors.initials: LB
authors.lastname: Vensand
authors.forename: Carmen R
authors.initials: CR
authors.lastname: Dusenberry
authors.forename: Samson
authors.initials: S
authors.lastname: Jacob
authors.forename: Jean Paul G
authors.initials: JP
authors.lastname: Vonsattel
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Paull
authors.forename: Michael L
authors.initials: ML
authors.lastname: Shelanski
authors.affiliation: The New York Stem Cell Foundation Research Institute, New York, NY 10032, USA. jc2892@cumc.columbia.edu.
authors.forename: John F
authors.initials: JF
authors.lastname: Crary
authors.forename: Scott A
authors.initials: SA
authors.lastname: Noggle
mesh_terms: D000544:Alzheimer Disease / Q000473:pathology; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D002454:Cell Differentiation; D002461:Cell Line, Transformed / Q000473:pathology; D049109:Cell Proliferation; D019992:Databases as Topic; D004388:Dura Mater / Q000473:pathology*; D005347:Fibroblasts / Q000378:metabolism / Q000821:virology; D018398:Homeodomain Proteins / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000473:pathology / Q000502:physiology*; D051379:Mice; D000071317:Nanog Homeobox Protein; D019636:Neurodegenerative Diseases / Q000473:pathology; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D011180:Postmortem Changes; D011509:Proteoglycans / Q000378:metabolism; D012867:Skin / Q000166:cytology; D055746:Stage-Specific Embryonic Antigens / Q000378:metabolism
chemical_list: D000954:Antigens, Surface; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; D011509:Proteoglycans; D055746:Stage-Specific Embryonic Antigens; C107064:TRA-1-60 antigen, human; C066584:stage-specific embryonic antigen-4