title: Intra-articular administration of hyaluronic acid increases the volume of the hyaline cartilage regenerated in a large osteochondral defect by implantation of a double-network gel.
abstract: Implantation of PAMPS/PDMAAm double-network (DN) gel can induce hyaline cartilage regeneration in the osteochondral defect. However, it is a problem that the volume of the regenerated cartilage tissue is gradually reduced at 12 weeks. This study investigated whether intra-articular administration of hyaluronic acid (HA) increases the volume of the cartilage regenerated with the DN gel at 12 weeks. A total of 48 rabbits were used in this study. A cylindrical osteochondral defect created in the bilateral femoral trochlea was treated with DN gel (Group DN) or left without any implantation (Group C). In both Groups, we injected 1.0 mL of HA in the left knee, and 1.0 mL of saline solution in the right knee. Quantitative histological evaluations were performed at 2, 4, and 12 weeks, and PCR analysis was performed at 2 and 4 weeks after surgery. In Group DN, the proteoglycan-rich area was significantly greater in the HA-injected knees than in the saline-injected knees at 12 weeks (P = 0.0247), and expression of type 2 collagen, aggrecan, and Sox9 mRNAs was significantly greater in the HA-injected knees than in the saline-injected knees at 2 weeks (P = 0.0475, P = 0.0257, P = 0.0222, respectively). The intra-articular administration of HA significantly enhanced these gene expression at 2 weeks and significantly increased the volume of the hyaline cartilage regenerated by implantation of a DN gel at 12 weeks. This information is important to develop an additional method to increase the volume of the hyaline cartilage tissue in a potential cartilage regeneration strategy using the DN gel.
authors.affiliation: Department of Sports Medicine and Joint Surgery, Hokkaido University Graduate School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo, 060-8638, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Fukui
authors.forename: Nobuto
authors.initials: N
authors.lastname: Kitamura
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kurokawa
authors.forename: Masashi
authors.initials: M
authors.lastname: Yokota
authors.forename: Eiji
authors.initials: E
authors.lastname: Kondo
authors.forename: Jian Ping
authors.initials: JP
authors.lastname: Gong
authors.forename: Kazunori
authors.initials: K
authors.lastname: Yasuda
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D053674:Aggrecans / Q000235:genetics; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D020219:Chondrogenesis / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D004334:Drug Administration Schedule; D005260:Female; D005782:Gels; D015870:Gene Expression / Q000187:drug effects; D051457:Hyaline Cartilage / Q000187:drug effects* / Q000293:injuries* / Q000503:physiopathology; D006820:Hyaluronic Acid / Q000008:administration & dosage*; D007270:Injections, Intra-Articular; D008422:Materials Testing; D011108:Polymers / Q000737:chemistry; D011509:Proteoglycans / Q000378:metabolism; D011817:Rabbits; D012038:Regeneration / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D055758:SOX9 Transcription Factor / Q000235:genetics; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D000178:Acrylamides; D053674:Aggrecans; D001672:Biocompatible Materials; D024043:Collagen Type II; D005782:Gels; D011108:Polymers; D011509:Proteoglycans; D055758:SOX9 Transcription Factor; D013451:Sulfonic Acids; C429790:poly(N,N-dimethylacrylamide); C077118:poly(2-acrylamido-2-methyl-1-propanesulfonic acid); D006820:Hyaluronic Acid