title: Identification and characterization of the serine/threonine protein kinases in Bifidobacterium.
abstract: Six genes encoding the bifidobacterial Hanks-type (eukaryote-like) serine/threonine protein kinases (STPK) were identified and classified. The genome of each bifidobacterial strain contains four conserved genes and one species-specific gene. Bifidobacterium longum and Bifidobacterium bifidum possess the unique gene found only in these species. The STPK genes of Russian industrial probiotic strain B. longum B379M were cloned and sequenced. The expression of these genes in Escherichia coli and bifidobacteria was observed. Autophosphorylation of the conserved STPK Pkb5 and species-specific STPK Pkb2 was demonstrated. This is the first report on Hanks-type STPK in bifidobacteria.
authors.affiliation: Department of Genetics of Microorganisms, Vavilov Institute of General Genetics, Russian Academy of Sciences, 3 Gubkin Street, 119991, Moscow, Russia, veneranez@rambler.ru.
authors.forename: Venera Z
authors.initials: VZ
authors.lastname: Nezametdinova
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Zakharevich
authors.forename: Maria G
authors.initials: MG
authors.lastname: Alekseeva
authors.forename: Olga V
authors.initials: OV
authors.lastname: Averina
authors.forename: Dilara A
authors.initials: DA
authors.lastname: Mavletova
authors.forename: Valery N
authors.initials: VN
authors.lastname: Danilenko
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001644:Bifidobacterium / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D020134:Catalytic Domain; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010766:Phosphorylation; D010802:Phylogeny; D019936:Probiotics; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D017346:Protein Serine-Threonine Kinases