title: Cigarette smoke induces alterations in the drug-binding properties of human serum albumin.
abstract: Albumin is the most abundant plasma protein and serves as a transport and depot protein for numerous endogenous and exogenous compounds. Earlier we had shown that cigarette smoke induces carbonylation of human serum albumin (HSA) and alters its redox state. Here, the effect of whole-phase cigarette smoke on HSA ligand-binding properties was evaluated by equilibrium dialysis and size-exclusion HPLC or tryptophan fluorescence. The binding of salicylic acid and naproxen to cigarette smoke-oxidized HSA resulted to be impaired, unlike that of curcumin and genistein, chosen as representative ligands. Binding of the hydrophobic fluorescent probe 4,4'-bis(1-anilino-8-naphtalenesulfonic acid) (bis-ANS), intrinsic tryptophan fluorescence, and susceptibility to enzymatic proteolysis revealed slight changes in albumin conformation. These findings suggest that cigarette smoke-induced modifications of HSA may affect the binding, transport and bioavailability of specific ligands in smokers.
authors.affiliation: Department of Biosciences, Università degli Studi di Milano, Milan, Italy; Department of Biomedical Sciences for Health, Università degli Studi di Milano, Milan, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Clerici
authors.affiliation: Department of Biosciences, Università degli Studi di Milano, Milan, Italy.
authors.forename: Graziano
authors.initials: G
authors.lastname: Colombo
authors.affiliation: Istituto di Chimica del Riconoscimento Molecolare, CNR, Milan, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Secundo
authors.affiliation: Department of Biomedical Sciences for Health, Università degli Studi di Milano, Milan, Italy.
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Gagliano
authors.affiliation: Department of Biosciences, Università degli Studi di Milano, Milan, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Colombo
authors.affiliation: Department of Medical Biotechnology and Translational Medicine, Clinica ortopedica e traumatologica, Università degli Studi di Milano and Istituto Clinico Humanitas, Rozzano, Milan, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Portinaro
authors.affiliation: Department of Life Sciences, University of Siena, Siena, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Giustarini
authors.affiliation: Department of Biosciences, Università degli Studi di Milano, Milan, Italy.
authors.forename: Aldo
authors.initials: A
authors.lastname: Milzani
authors.affiliation: Department of Life Sciences, University of Siena, Siena, Italy.
authors.forename: Ranieri
authors.initials: R
authors.lastname: Rossi
authors.affiliation: Department of Biosciences, Università degli Studi di Milano, Milan, Italy. Electronic address: quack@unimi.it.
authors.forename: Isabella
authors.initials: I
authors.lastname: Dalle-Donne
mesh_terms: D003474:Curcumin / Q000737:chemistry / Q000378:metabolism; D019833:Genistein / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D008024:Ligands; D009288:Naproxen / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D011485:Protein Binding; D011487:Protein Conformation; D059748:Proteolysis; D020156:Salicylic Acid / Q000737:chemistry / Q000378:metabolism; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D012906:Smoke / Q000009:adverse effects*; D012907:Smoking / Q000009:adverse effects*
chemical_list: D008024:Ligands; D012709:Serum Albumin; D012906:Smoke; C001702:mercaptoalbumin; D009288:Naproxen; D019833:Genistein; D003474:Curcumin; D020156:Salicylic Acid
keywords: Cigarette smoke; Human serum albumin; Naproxen; Oxidation; Protein conformation; Salicylic acid