title: Characterization of high-molecular-weight glutenin subunits from Eremopyrum bonaepartis and identification of a novel variant with unusual high molecular weight and altered cysteine residues.
abstract: We characterized two high-molecular-weight glutenin subunit (HMW-GS) variants from Eremopyrum bonaepartis, determined their complete open reading frames, and further expressed them in a bacterial system. The variants have many novel structural features compared with typical subunits encoded by Glu-1 loci: 1Fx3.7 and 1Fy1.5 exhibit hybrid properties of x- and y-type subunits. In addition, unusual molecular mass and altered number and distribution of cysteine residues were unique features of HMW-GSs encoded by Glu-F1 from E. bonaepartis. The mature 1Fx3.7 subunit has a full length of 1,223 amino acid residues, making it the largest subunit found thus far, while 1Fy1.5 is just 496 residues. In addition, the mutated PGQQ repeat motif was found in the repetitive region of 1Fx3.7. Although it has a similar molecular mass to that previously reported for 1Dx2.2, 1Dx2.2* and 1S(sh)x2.9 subunits, 1Fx3.7 appears to have had a different evolutionary history. The N-terminal and repetitive regions have a total of four additional cysteine residues, giving 1Fx3.7 a total of eight cysteines, while 1Fy1.5 has only six cysteines because the GHCPTSPQQ nonapeptide at the end of the repetitive region is deleted. With its extra cysteine residues and the longest repetitive region, features that are relevant to good wheat quality, the 1Fx3.7 subunit gene could be an excellent candidate for applications in wheat quality improvement.
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
authors.forename: Qian-Tao
authors.initials: QT
authors.lastname: Jiang
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Ma
authors.forename: Long
authors.initials: L
authors.lastname: Wei
authors.forename: Shan
authors.initials: S
authors.lastname: Zhao
authors.forename: Quan-Zhi
authors.initials: QZ
authors.lastname: Zhao
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Qi
authors.forename: Zhen-Xiang
authors.initials: ZX
authors.lastname: Lu
authors.forename: You-Liang
authors.initials: YL
authors.lastname: Zheng
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Wei
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003545:Cysteine / Q000378:metabolism; D019143:Evolution, Molecular; D014644:Genetic Variation; D005983:Glutens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D005810:Multigene Family; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D006109:Poaceae / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D005983:Glutens; C037302:glutenin; D003545:Cysteine