title: The selenocysteine incorporation machinery allows the dual use of sense codons: a new strategy for expanding the genetic code?
abstract: Sense and Secis: Even though selenocysteine, Sec, is naturally incorporated by suppressing UAG stop codons, it was recently shown that sense codons such as UAC can efficiently code for Sec. This article highlights the implications of using such a strategy to introduce unnatural amino acids site-selectively into proteins.
authors.affiliation: Interfaculty Institute of Biochemistry, Universität Tübingen, Auf der Morgenstelle 15, 72076 Tübingen (Germany) http://www.ifib.uni-tuebingen.de/research/stafforst.html. thorsten.stafforst@uni-tuebingen.de.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Stafforst
mesh_terms: D003062:Codon; D018388:Codon, Terminator; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism; D005560:Formate Dehydrogenases / Q000737:chemistry / Q000378:metabolism; D005815:Genetic Code; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000378:metabolism; D017279:Selenocysteine / Q000378:metabolism*
chemical_list: D003062:Codon; D018388:Codon, Terminator; D029968:Escherichia coli Proteins; D017279:Selenocysteine; D012343:RNA, Transfer; D005560:Formate Dehydrogenases
keywords: genetic code; seleno proteins; selenocysteine; tRNA; translation