title: SHOOT GROWTH1 maintains Arabidopsis epigenomes by regulating IBM1.
abstract: Maintaining correct DNA and histone methylation patterns is essential for the development of all eukaryotes. In Arabidopsis, we identified SHOOT GROWTH1 (SG1), a novel protein involved in the control of gene methylation. SG1 contains both a Bromo-Adjacent Homology (BAH) domain found in several chromatin regulators and an RNA-Recognition Motif (RRM). The sg1 mutations are associated with drastic pleiotropic phenotypes. The mutants degenerate after few generations and are similar to mutants of the histone demethylase INCREASE IN BONSAI METHYLATION1 (IBM1). A methylome analysis of sg1 mutants revealed a large number of gene bodies hypermethylated in the cytosine CHG context, associated with an increase in di-methylation of lysine 9 on histone H3 tail (H3K9me2), an epigenetic mark normally found in silenced transposons. The sg1 phenotype is suppressed by mutations in genes encoding the DNA methyltransferase CHROMOMETHYLASE3 (CMT3) or the histone methyltransferase KRYPTONITE (KYP), indicating that SG1 functions antagonistically to CMT3 or KYP. We further show that the IBM1 transcript is not correctly processed in sg1, and that the functional IBM1 transcript complements sg1. Altogether, our results suggest a function for SG1 in the maintenance of genome integrity by regulating IBM1.
authors.affiliation: INRA, UMR1318, Institut Jean-Pierre Bourgin, RD10, Versailles, France ; AgroParisTech, Institut Jean-Pierre Bourgin, RD10, Versailles, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Coustham
authors.affiliation: INRA, UMR1318, Institut Jean-Pierre Bourgin, RD10, Versailles, France ; AgroParisTech, Institut Jean-Pierre Bourgin, RD10, Versailles, France.
authors.forename: Daniela
authors.initials: D
authors.lastname: Vlad
authors.affiliation: INRA, UMR1318, Institut Jean-Pierre Bourgin, RD10, Versailles, France ; AgroParisTech, Institut Jean-Pierre Bourgin, RD10, Versailles, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Deremetz
authors.affiliation: INRA, UMR1318, Institut Jean-Pierre Bourgin, RD10, Versailles, France ; AgroParisTech, Institut Jean-Pierre Bourgin, RD10, Versailles, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Gy
authors.affiliation: INRA, UMR1318, Institut Jean-Pierre Bourgin, RD10, Versailles, France ; AgroParisTech, Institut Jean-Pierre Bourgin, RD10, Versailles, France.
authors.forename: Francisco A
authors.initials: FA
authors.lastname: Cubillos
authors.affiliation: INRA, UMR1318, Institut Jean-Pierre Bourgin, RD10, Versailles, France ; AgroParisTech, Institut Jean-Pierre Bourgin, RD10, Versailles, France.
authors.forename: Envel
authors.initials: E
authors.lastname: Kerdaffrec
authors.affiliation: INRA, UMR1318, Institut Jean-Pierre Bourgin, RD10, Versailles, France ; AgroParisTech, Institut Jean-Pierre Bourgin, RD10, Versailles, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Loudet
authors.affiliation: INRA, UMR1318, Institut Jean-Pierre Bourgin, RD10, Versailles, France ; AgroParisTech, Institut Jean-Pierre Bourgin, RD10, Versailles, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bouché
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D018506:Gene Expression Regulation, Plant; D023061:Gene Order; D018745:Genome, Plant; D006657:Histones / Q000378:metabolism; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics*; D009154:Mutation; D010641:Phenotype; D016601:RNA-Binding Proteins / Q000378:metabolism*; D015398:Signal Transduction; D059467:Transcriptome
chemical_list: C584540:ASI1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D006657:Histones; D016601:RNA-Binding Proteins; C526313:IBM1 protein, Arabidopsis; D056484:Jumonji Domain-Containing Histone Demethylases