title: Molecular epidemiology of Enterobacteriaceae that produce VIMs and IMPs from the SMART surveillance program.
abstract: A study was designed to characterize 35 non-repeat isolates of VIM- and IMP-producing Enterobacteriaceae obtained from the SMART surveillance program. Characterization was done by polymerase chain reaction, sequencing, and multi-locus sequencing. The VIM-1, -2, -5, -26, -27, -33, and IMP-1 and -26-producing Enterobacteriaceae were obtained from Greece, Italy, Spain, Philippines, Turkey, Australia, Mexico, USA, and India. Plasmids varied in size from 60 to 300 kb and belonged to IncA/C, IncF, IncHI1, IncL/M, IncN, and IncK incompatibility groups. The most common gene cassettes consisted of blaIMP-26, qacG, aacA4 and blaVIM, aacA7, dhfrI, and aadA1. Intercountry, interhospital, intrahospital, interspecies, and intraclonal spread of blaVIM and blaIMP containing plasmids and sequence types (STs) occurred in Greece, Italy, Spain, and Philippines. ST147 with IncA/C and IncF plasmids is an important drug-resistant ST among Klebsiella pneumoniae with VIMs. Our study highlights the importance of surveillance programs using molecular techniques as powerful tools to identify the transmission of STs with their respective plasmids.
authors.affiliation: Division of Microbiology, Calgary Laboratory Services, University of Calgary, Calgary, Alberta, Canada; Departments of Pathology & Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Gisele
authors.initials: G
authors.lastname: Peirano
authors.affiliation: International Health Management Associates Inc., Schaumburg, IL, USA.
authors.forename: Christine
authors.initials: C
authors.lastname: Lascols
authors.affiliation: International Health Management Associates Inc., Schaumburg, IL, USA.
authors.forename: Meredith
authors.initials: M
authors.lastname: Hackel
authors.affiliation: International Health Management Associates Inc., Schaumburg, IL, USA; Diagnostic Services of Manitoba, University of Manitoba, Winnipeg, Manitoba, Canada; Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Daryl J
authors.initials: DJ
authors.lastname: Hoban
authors.affiliation: Division of Microbiology, Calgary Laboratory Services, University of Calgary, Calgary, Alberta, Canada; Departments of Pathology & Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada; Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada. Electronic address: johann.pitout@cls.ab.ca.
authors.forename: Johann D D
authors.initials: JD
authors.lastname: Pitout
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000145:classification / Q000187:drug effects* / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology / Q000379:methods*; D058885:Multilocus Sequence Typing; D010957:Plasmids; D016133:Polymerase Chain Reaction; D015363:Quinolones / Q000494:pharmacology; D012336:RNA, Ribosomal, 16S / Q000302:isolation & purification; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D015363:Quinolones; D012336:RNA, Ribosomal, 16S; D001618:beta-Lactamases
keywords: Enterobacteriaceae; IMP; Molecular epidemiology; VIM