title: [Expressions of differential proteins in the expressed prostatic secretion of patients with III A chronic prostatitis].
abstract: OBJECTIVE
  To study the expressions of differential proteins in the expressed prostatic secretion (EPS) of patients with III A chronic prostatitis and healthy men.
  
  
  METHODS
  We collected EPS samples from 35 patients with III A chronic prostatitis and 18 age-matched healthy men, and detected the differentially expressed proteins in EPS by MALDI-TOF/MS. Based on the data obtained, we conducted a statistical analysis on the mass-to-charge (m/z) ratios of different proteins and a retrieval analysis on the relevant proteins using the protein database.
  
  
  RESULTS
  In the comparative studies of the III A chronic prostatitis patients and healthy men, 5 proteins were detected as at least 2-fold differentially expressed, which were probably brevinin-2Eg, big endothelin-1, alpha-defensin 15, beta-defensin 134 and prostatic steroid-binding protein C2. The m/z ratios were significantly up-regulated in 3 372, 3 487, 425 and 5 325 Da proteins (P < 0.01) and down-regulated in 10631Da (P < 0.01).
  
  
  CONCLUSIONS
  Proteins are differentially expressed in the EPS of III A chronic prostatitis patients and healthy men, and these proteins may be significantly correlated with the development and progression of III A chronic prostatitis.
authors.affiliation: Department of Andrology, The First Hospital of Jilin University, Changchun, Jilin 130021, China.
authors.forename: Xiao-Fan
authors.initials: XF
authors.lastname: Dai
authors.affiliation: Department of Andrology, The First Hospital of Jilin University, Changchun, Jilin 130021, China.
authors.forename: Kai-Min
authors.initials: KM
authors.lastname: Guo
authors.affiliation: Center of Reproductive Medicine, The First Hospital of Zhejiang University, Hangzhou, Zhejiang 310003, China.
authors.forename: Cong-Qi
authors.initials: CQ
authors.lastname: Du
authors.affiliation: Department of Andrology, The First Hospital of Jilin University, Changchun, Jilin 130021, China.
authors.forename: Ling-Yun
authors.initials: LY
authors.lastname: Liu
authors.affiliation: Department of Andrology, The First Hospital of Jilin University, Changchun, Jilin 130021, China.
authors.forename: Fu-Biao
authors.initials: FB
authors.lastname: Li
authors.affiliation: Department of Andrology, The First Hospital of Jilin University, Changchun, Jilin 130021, China.
authors.forename: Hong-Liang
authors.initials: HL
authors.lastname: Wang
mesh_terms: D000328:Adult; D001826:Body Fluids / Q000378:metabolism*; D016022:Case-Control Studies; D002908:Chronic Disease; D023082:Defensins / Q000378:metabolism; D019332:Endothelin-1 / Q000378:metabolism; D006801:Humans; D008297:Male; D011467:Prostate / Q000378:metabolism*; D011472:Prostatitis / Q000145:classification / Q000378:metabolism*; D055815:Young Adult
chemical_list: D023082:Defensins; D019332:Endothelin-1