title: Effects of sewage discharges on lipid and fatty acid composition of the Patagonian bivalve Diplodon chilensis.
abstract: Lipid and fatty acid (FA) composition and selected oxidative stress parameters of freshwater clams (Dipolodon chilensis), from a sewage-polluted (SMA) and a clean site, were compared. Trophic markers FA were analyzed in clams and sediment. Saturated FA (SAFA), and bacteria and sewage markers were abundant in SMA sediments, while diatom markers were 50% lower. Proportions of SAFA, branched FA, 20:5n-3 (EPA) and 22:6n-3 (DHA) were higher in SMA clams. Chronic exposure of D. chilensis to increasing eutrophication affected its lipid and FA composition. The increase in EPA and DHA proportions could be an adaptive response, which increases stress resistance but could also lead to higher susceptibility to lipid peroxidation TBARS, lipofuscins (20-fold) and GSH concentrations were higher in SMA clams. FA markers indicated terrestrial plant detritus and bacteria are important items in D. chilensis diet. Anthropogenic input in their food could be traced using specific FA as trophic markers.
authors.affiliation: Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, INQUIBICEN-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Pab. II, Ciudad Universitaria, 1428 Buenos Aires, Argentina; Laboratorio de Ecotoxicología Acuática, INIBIOMA (CONICET-UNCo). CEAN, ruta prov. 61 km 3. 8371, Junín de los Andes, Neuquén, Argentina. Electronic address: Iara.Rocchetta@awi.de.
authors.forename: Iara
authors.initials: I
authors.lastname: Rocchetta
authors.affiliation: Instituto de Investigaciones Bioquímicas de la Plata (INIBIOLP), Facultad de Ciencias Médicas, Universidad Nacional de La Plata - CONICET CCT La Plata, Av. 60 y 120, 1900 La Plata, Argentina.
authors.forename: María Y
authors.initials: MY
authors.lastname: Pasquevich
authors.affiliation: Instituto de Investigaciones Bioquímicas de la Plata (INIBIOLP), Facultad de Ciencias Médicas, Universidad Nacional de La Plata - CONICET CCT La Plata, Av. 60 y 120, 1900 La Plata, Argentina.
authors.forename: Horacio
authors.initials: H
authors.lastname: Heras
authors.affiliation: Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, INQUIBICEN-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Pab. II, Ciudad Universitaria, 1428 Buenos Aires, Argentina.
authors.forename: María del Carmen
authors.initials: Mdel C
authors.lastname: Ríos de Molina
authors.affiliation: Laboratorio de Ecotoxicología Acuática, INIBIOMA (CONICET-UNCo). CEAN, ruta prov. 61 km 3. 8371, Junín de los Andes, Neuquén, Argentina.
authors.forename: Carlos M
authors.initials: CM
authors.lastname: Luquet
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D049872:Bivalvia / Q000502:physiology*; D004784:Environmental Monitoring / Q000379:methods; D005227:Fatty Acids / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D012722:Sewage / Q000737:chemistry / Q000706:statistics & numerical data; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014873:Water Pollutants / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D005227:Fatty Acids; D012722:Sewage; D017392:Thiobarbituric Acid Reactive Substances; D014873:Water Pollutants
keywords: Domestic effluents; Fatty acid markers; Freshwater bivalve; Lipofuscins; Oxidative stress; Pollution