title: Functional characteristics of porcine peripheral T cells stimulated with IL-2 or IL-2 and PMA.
abstract: In human or mouse, mature T cells express either CD4 or CD8, resulting in different functions in the periphery. Interestingly, porcine CD4 and CD8 double positive (DP) T cells are present in the blood, and their proportions change from youth to adulthood. However, the features of these cells in swine are poorly understood. We investigated the fate of porcine peripheral T cells based on their functional characteristics, including proliferation and the expression of CD4 and CD8 co-receptors. The results showed that all the populations changed their CD8 expression in a time-dependent manner and porcine T cells had different proliferative pattern from human T cells. The results further revealed that Th2 cytokines were increased later in porcine T cells compared to human T cells upon stimulation with IL-2+PMA. Collectively, we found that the fate of porcine peripheral T cells is different from that of human T cells, and the changes occur in a time- and stimulation-dependent manner.
authors.affiliation: Department of Agricultural Biotechnology, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea; Laboratory Sciences Division, International Vaccine Institute, Seoul, Republic of Korea.
authors.forename: In Su
authors.initials: IS
authors.lastname: Cheon
authors.affiliation: Department of Agricultural Biotechnology, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea; Laboratory Sciences Division, International Vaccine Institute, Seoul, Republic of Korea; Center for Food and Bioconvergence, Seoul National University, Seoul, Republic of Korea.
authors.forename: Sung-Moo
authors.initials: SM
authors.lastname: Park
authors.affiliation: Department of Agricultural Biotechnology, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biotechnology, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Hong
authors.affiliation: Department of Agricultural Biotechnology, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea; National Institute of Animal Science, RDA, Republic of Korea.
authors.forename: Sang Yun
authors.initials: SY
authors.lastname: Ji
authors.affiliation: Department of Agricultural Biotechnology, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea; Laboratory Sciences Division, International Vaccine Institute, Seoul, Republic of Korea.
authors.forename: Byoung-Shik
authors.initials: BS
authors.lastname: Shim
authors.affiliation: Department of Agricultural Biotechnology, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Kwang Ho
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biotechnology, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Pil Seung
authors.initials: PS
authors.lastname: Heo
authors.affiliation: Department of Agricultural Biotechnology, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Yoo Yong
authors.initials: YY
authors.lastname: Kim
authors.affiliation: National Institute of Animal Science, RDA, Republic of Korea.
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Jung
authors.affiliation: Department of Biological Science and Technology, Yonsei University, Wonju, Republic of Korea.
authors.forename: Hakhyun
authors.initials: H
authors.lastname: Ka
authors.affiliation: Department of Oral Microbiology & Immunology, Dental Research Institute, BK21 Program, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Han
authors.affiliation: Laboratory Sciences Division, International Vaccine Institute, Seoul, Republic of Korea. Electronic address: mksong@ivi.int.
authors.forename: Manki
authors.initials: M
authors.lastname: Song
authors.affiliation: Department of Agricultural Biotechnology, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea; Center for Food and Bioconvergence, Seoul National University, Seoul, Republic of Korea. Electronic address: cyun@snu.ac.kr.
authors.forename: Cheol-Heui
authors.initials: CH
authors.lastname: Yun
mesh_terms: D056704:Adaptive Immunity / Q000276:immunology*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D005434:Flow Cytometry / Q000662:veterinary; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology*; D007963:Leukocytes, Mononuclear; D008213:Lymphocyte Activation / Q000276:immunology*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine / Q000097:blood / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000276:immunology
chemical_list: D016207:Cytokines; D007376:Interleukin-2; D012333:RNA, Messenger; D013755:Tetradecanoylphorbol Acetate
keywords: CD4(+)CD8(+) double positive; Human; Porcine; T cells