title: Bex2 controls proliferation of human glioblastoma cells through NF-κB signaling pathway.
abstract: Glioblastoma is the most common and fatal human brain malignancy in adults with highly proliferative capacity. Despite advances in surgery and adjuvant therapy, the median survival of patients has changed little over recent decades. Identifying molecules critical for glioma development is significant for devising effective targeted therapy. We previously reported that Bex2, a member of the brain expressed X-linked gene family, promoted the progression of glioma by promoting cell proliferation. In the present study, we investigated the main mechanism of Bex2 promoting the proliferation of glioblastoma cells. We found that Bex2 downregulation inhibited glioma cell proliferation and the expression of NF-κB p65, but Bex2 overexpression promoted them. Similarly, the proliferation of glioma cells was inhibited by p65 downregulation but increased by p65 overexpression. In addition, Bex2 overexpression-induced cell proliferation was abolished by p65 downregulation. Furthermore, Bex2 with nuclear localization signal deleted no longer promoted p65 expression. In conclusion, this study demonstrates that Bex2 promotes proliferation of human glioblastoma cells via NF-κB signaling pathway and Bex2 nuclear location is critical for p65 expression.
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Xuzhou Medical College, 99 West Huai-hai Road, Xuzhou, Jiangsu, 221002, People's Republic of China.
authors.forename: Qingming
authors.initials: Q
authors.lastname: Meng
authors.forename: Tongle
authors.initials: T
authors.lastname: Zhi
authors.forename: Yuewen
authors.initials: Y
authors.lastname: Chao
authors.forename: Er
authors.initials: E
authors.lastname: Nie
authors.forename: Xuebin
authors.initials: X
authors.lastname: Xu
authors.forename: Qiong
authors.initials: Q
authors.lastname: Shi
authors.forename: Lei
authors.initials: L
authors.lastname: Hua
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Wenjian
authors.initials: W
authors.lastname: Zhan
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiuping
authors.initials: X
authors.lastname: Zhou
authors.forename: Rutong
authors.initials: R
authors.lastname: Yu
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000378:metabolism*; D006801:Humans; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism*
chemical_list: C530662:BEX2 protein, human; D009419:Nerve Tissue Proteins; D051996:Transcription Factor RelA