title: Lack of association between cyclooxygenase 2-765G/C gene polymorphism and breast cancer risk in Ahvaz, west-south Iran.
abstract: Cyclooxygenases are key enzymes in conversion of arachidonic acid into prostaglandin H2. Cyclooxygenase-2 (COX-2) increases prostaglandins in neoplastic tissue. COX-2 has important roles in cell proliferation cancers, angiogenesis, and alzheimer. COX-2 is up-regulated in several types of cancer, and it is hypothesized that COX-2 expression may be genetically influenced. Our main objective was to evaluated the association of polymorphism COX-2 with risk of breast cancer in khouzestan province, and the second objective of the study was to evaluate the association with biochemistry parameters. This study consisting of 150 patients with breast cancer and 120 normal DNA was extracted from the white blood cells. Polymorphism cox2 gene was detected by polymerase chain reaction according to the standard methods. The profile lipids and estrogen were measured in two groups by standard methods. Chi square analysis showed that there was no association between breast cancer risk and COX-2 -765G>C genotype and alleles. Also, no association were observed between -765G>C polymorphism and biochemistry parameters. A multiple logistic regression model with cox2 genotypes and LDL and HDL as covariates revealed that there is no significant association between cox2 genotypes and risk of breast cancer, but higher values of LDL and HDL significantly increase risk of breast cancer.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Ahvaz Jondishapour University of Medical Sciences, Ahvaz, Iran.
authors.forename: Behnaz
authors.initials: B
authors.lastname: Ahmadi
authors.forename: Abdolrahim
authors.initials: A
authors.lastname: Nikzamir
authors.forename: Seid Mohamadali
authors.initials: SM
authors.lastname: Ghafari
authors.forename: Ghorban
authors.initials: G
authors.lastname: Mohamadzadeh
authors.forename: Mahmod
authors.initials: M
authors.lastname: Latifi
authors.forename: Ahmad
authors.initials: A
authors.lastname: Bafandeh
authors.forename: Mohammad
authors.initials: M
authors.lastname: Fathi
authors.forename: Mir Saeed
authors.initials: MS
authors.lastname: Yekaninejad
authors.forename: Mahfam
authors.initials: M
authors.lastname: Nikzamir
mesh_terms: D000328:Adult; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D049109:Cell Proliferation; D008076:Cholesterol, HDL / Q000097:blood / Q000235:genetics; D008078:Cholesterol, LDL / Q000097:blood / Q000235:genetics; D051546:Cyclooxygenase 2 / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D007492:Iran; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
chemical_list: D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human