title: Use of magnetic folate-dextran-retinoic acid micelles for dual targeting of doxorubicin in breast cancer.
abstract: Amphiphilic copolymer of folate-conjugated dextran/retinoic acid (FA/DEX-RA) was self-assembled into micelles by direct dissolution method. Magnetic iron oxide nanoparticles (MNPs) coated with oleic acid (OA) were prepared by hydrothermal method and encapsulated within the micelles. Doxorubicin HCl was loaded in the magnetic micelles. The characteristics of the magnetic micelles were determined by Fourier transform infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), transmission electron microscopy (TEM), and vibrating sample magnetometer (VSM). The crystalline state of OA-coated MNPs and their heat capacity were analyzed by X-ray diffraction (XRD) and differential scanning calorimetry (DSC) methods, respectively. The iron content of magnetic micelles was determined using inductively coupled plasma optical emission spectrometry (ICP-OES). Bovine serum albumin (BSA) was used to test the protein binding of magnetic micelles. The cytotoxicity of doxorubicin loaded magnetic micelles was studied on MCF-7 and MDA-MB-468 cells using MTT assay and their quantitative cellular uptake by fluorimetry method. TEM results showed the MNPs in the hydrophobic core of the micelles. TGA results confirmed the presence of OA and FA/DEX-RA copolymer on the surface of MNPs and micelles, respectively. The magnetic micelles showed no significant protein bonding and reduced the IC₅₀ of the drug to about 10 times lower than the free drug.
authors.affiliation: Department of Pharmaceutics, School of Pharmacy and Novel Drug Delivery Systems Research Centre, Isfahan University of Medical Sciences, Isfahan 81745-359, Iran.
authors.forename: J
authors.initials: J
authors.lastname: Varshosaz
authors.affiliation: Department of Biotechnology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan 81745-359, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Sadeghi-aliabadi
authors.affiliation: Department of Pharmaceutics, School of Pharmacy and Novel Drug Delivery Systems Research Centre, Isfahan University of Medical Sciences, Isfahan 81745-359, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Ghasemi
authors.affiliation: Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
authors.forename: B
authors.initials: B
authors.lastname: Behdadfar
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D003911:Dextrans / Q000008:administration & dosage / Q000737:chemistry; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry; D016503:Drug Delivery Systems; D005260:Female; D005290:Ferric Compounds / Q000008:administration & dosage / Q000737:chemistry; D005492:Folic Acid / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D061986:MCF-7 Cells; D058185:Magnetite Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D008823:Micelles; D014212:Tretinoin / Q000008:administration & dosage / Q000737:chemistry
chemical_list: D003911:Dextrans; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D008823:Micelles; C000499:ferric oxide; D014212:Tretinoin; D004317:Doxorubicin; D005492:Folic Acid