title: Probing physical properties of a DNA-protein complex using nanofluidic channels.
abstract: A method to investigate physical properties of a DNA-protein complex in solution is demonstrated. By using tapered nanochannels and lipid passivation the persistence length of a RecA filament formed on double-stranded DNA is determined to 1.15 μm, in agreement with the literature, without attaching protein or DNA to any handles or surfaces.
authors.affiliation: Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-412 96, Gothenburg, Sweden.
authors.forename: Karolin
authors.initials: K
authors.lastname: Frykholm
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Alizadehheidari
authors.forename: Joachim
authors.initials: J
authors.lastname: Fritzsche
authors.forename: Jens
authors.initials: J
authors.lastname: Wigenius
authors.forename: Mauro
authors.initials: M
authors.lastname: Modesti
authors.forename: Fredrik
authors.initials: F
authors.lastname: Persson
authors.forename: Fredrik
authors.initials: F
authors.lastname: Westerlund
mesh_terms: D055592:Biophysical Phenomena; D004247:DNA / Q000737:chemistry*; D004270:DNA, Circular / Q000378:metabolism; D044085:Microfluidics / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D011938:Rec A Recombinases / Q000737:chemistry*
chemical_list: D004270:DNA, Circular; D004247:DNA; D011938:Rec A Recombinases
keywords: DNA; fluorescence; lipid passivation; nanofluidics; proteins