title: Key architectural changes in tumor-negative lymph nodes from metastatic-free oral cancer patients are valuable prognostic factors.
abstract: Regional lymph node (LN) metastasis in oral cancer patients is the most significant grave prognostic factor. We evaluated the relationship between clinical outcomes and different histopathological changes in tumor-negative LNs (LN0) selected from neck dissections without metastatic disease (pN0). A total of 435 LN0 selected from pN0 neck dissections (up to three nodes in each level) were scored for histopathological parameters of LN areas, capsule thickness, subcapsular and medullary sinus ectasia, lobular architecture and percent of cortical reactive follicles. These were compared to 328 LN0 selected from neck dissections with metastases (pN+) after exclusion of metastatic LNs. Data were presented by maximum scores of each parameter in I-III (close) and in IV-V (distant) levels. Limited data from level V and regression analyses inferred that the values in level IV represented the worst changes for most patients. Cox proportional hazard regression on each parameter in close and distant levels demonstrated that capsule thickness, number of lobules and percent of reactive follicles were significantly associated with time to death from disease. The higher the change in distant levels, the shorter the time to death, while the higher the change in close levels (given a stable change in distant levels), the longer the time to death. After adjustment for gender, age and location, only the effect of the percent of reactive follicles retained their significant effect. Logistic regression of metastases demonstrated that all parameters except for percent of reactive follicles were significantly associated with risk of metastases, with differences between close and distant levels similar to those found for time to death. After adjustment for gender, age and location, only the area and number of lobes retained their significance. The findings of this study suggested that selective histopathological changes in tumor-negative LNs in metastatic-free patients provide new valuable prognostic parameters.
authors.affiliation: Institute of Pathology, The Chaim Sheba Medical Center, Tel Hashomer, Israel, lmy@netvision.net.il.
authors.forename: Marilena
authors.initials: M
authors.lastname: Vered
authors.forename: Ginette
authors.initials: G
authors.lastname: Schiby
authors.forename: Anna
authors.initials: A
authors.lastname: Schnaiderman-Shapiro
authors.forename: Ilya
authors.initials: I
authors.lastname: Novikov
authors.forename: Ibrahim O
authors.initials: IO
authors.lastname: Bello
authors.forename: Tuula
authors.initials: T
authors.lastname: Salo
authors.forename: Aleksi
authors.initials: A
authors.lastname: Rytkönen
authors.forename: Joonas H
authors.initials: JH
authors.lastname: Kauppila
authors.forename: Alex
authors.initials: A
authors.lastname: Dobriyan
authors.forename: Ran
authors.initials: R
authors.lastname: Yahalom
authors.forename: Shlomo
authors.initials: S
authors.lastname: Taicher
authors.forename: Dan
authors.initials: D
authors.lastname: Dayan
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology* / Q000601:surgery; D008207:Lymphatic Metastasis / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000401:mortality / Q000473:pathology* / Q000601:surgery; D009367:Neoplasm Staging; D011379:Prognosis; D016016:Proportional Hazards Models; D016896:Treatment Outcome