title: Feeding strategies for the improved biosynthesis of canthaxanthin from enzymatic hydrolyzed molasses in the fed-batch fermentation of Dietzia natronolimnaea HS-1.
abstract: The effect of two enzymatic hydrolyzed molasses (EHM)-feeding strategies including constant-(CFR) and exponential-(EFR) feeding rate on canthaxanthin (CTX) biosynthesis by Dietzia natronolimnaea HS-1 fed-batch fermentation was studied. The results showed that the CFR of 7 ml/h with an EHM content of 45 g/l led to the highest values of specific growth rate (0.127 h(-1)), biomass dry weight (17.66 g/l), total carotenoid (16.31 mg/l) and CTX (14.67 mg/l). A significant decrease in the kinetic growth and production parameters by the increasing EHM concentration from 30 to 60 g/l during EFR fed-batch bioprocess was observed (p<0.01). This study concluded that EHM alone can displace glucose-based medium towards improved CTX biosynthesis from D. natronolimnaea HS-1 using a CFR strategy during fed-batch culture.
authors.affiliation: Bioprocess Engineering Laboratory (BPEL), Department of Food Science, Engineering & Technology, Faculty of Agricultural Engineering and Technology, University of Tehran, P.O. Box 4111, Karaj 31587-77871, Iran.
authors.forename: Seyed Mohammad Taghi
authors.initials: SM
authors.lastname: Gharibzahedi
authors.affiliation: Bioprocess Engineering Laboratory (BPEL), Department of Food Science, Engineering & Technology, Faculty of Agricultural Engineering and Technology, University of Tehran, P.O. Box 4111, Karaj 31587-77871, Iran. Electronic address: srazavi@ut.ac.ir.
authors.forename: Seyed Hadi
authors.initials: SH
authors.lastname: Razavi
authors.affiliation: Bioprocess Engineering Laboratory (BPEL), Department of Food Science, Engineering & Technology, Faculty of Agricultural Engineering and Technology, University of Tehran, P.O. Box 4111, Karaj 31587-77871, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Mousavi
mesh_terms: D000192:Actinomycetales / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D061249:Batch Cell Culture Techniques / Q000379:methods*; D018533:Biomass; D016644:Canthaxanthin / Q000096:biosynthesis*; D002244:Carbon / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D008965:Molasses; D043324:beta-Fructofuranosidase / Q000378:metabolism*
chemical_list: D016644:Canthaxanthin; D002244:Carbon; D043324:beta-Fructofuranosidase
keywords: Canthaxanthin; Constant feeding rate; Dietzia natronolimnaea HS-1; High cell-density culture; Waste molasses