title: Rapid resistome fingerprinting and clonal lineage profiling of carbapenem-resistant Klebsiella pneumoniae isolates by targeted next-generation sequencing.
abstract: Thirty-two carbapenem-resistant Klebsiella pneumoniae isolates, representative of different resistance mechanisms and clonal lineages, were analyzed with the Pathogenica HAI BioDetection system, based on targeted next-generation sequencing (NGS) technology. With most strains, the system simultaneously yielded comprehensive information on relevant β-lactam resistance determinants and accurate discrimination of clonal lineages, in a shorter time frame and in a less labor-intensive manner than currently available methods for molecular epidemiology analysis. Results supported the usefulness of targeted NGS-based technologies for similar applications.
authors.affiliation: Department of Medical Biotechnologies, University of Siena, Siena, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Arena
authors.forename: P Alexander
authors.initials: PA
authors.lastname: Rolfe
authors.forename: Graeme
authors.initials: G
authors.lastname: Doran
authors.forename: Viola
authors.initials: V
authors.lastname: Conte
authors.forename: Sarah
authors.initials: S
authors.lastname: Gruszka
authors.forename: Thomas
authors.initials: T
authors.lastname: Clarke
authors.forename: Yemi
authors.initials: Y
authors.lastname: Adesokan
authors.forename: Tommaso
authors.initials: T
authors.lastname: Giani
authors.forename: Gian Maria
authors.initials: GM
authors.lastname: Rossolini
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015780:Carbapenems / Q000494:pharmacology*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D007711:Klebsiella pneumoniae / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D008826:Microbial Sensitivity Tests / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D058889:Molecular Typing / Q000379:methods*; D013997:Time Factors; D018440:beta-Lactam Resistance
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems