title: Identification and molecular characterization of an Akirin2 homolog in Chinese loach (Paramisgurnus dabryanus).
abstract: Akirin is a nuclear factor involved in innate immune responses of arthropods and mammals. In this study we have cloned an Akirin2 gene, pdakirin2, from freshwater Chinese loach (Paramisgurnus dabryanus) and characterized its biological functions. Phylogenetic analysis revealed deduced PdAkirin2 had high sequence identities to Akirin2 homologs from fish and mammals (70-91%), it contained two conserved nuclear localization signals (NLSs) with verified sub-cellular localization. Quantitative real-time (qRT)-PCR analysis indicated that PdAkirin2 was present in a wide range of loach tissues and showed up-regulation with challenges of Aeromonas hydrophila NJ-1, LPS and poly I:C. PdAkirin2 as an immune factor had significant effects on the expression of cytokines (TNFα, IFN-α, IFN-γ, IL-4 and IL-1β) and transcription factor NF-κB. This study provides insights into the potential role of PdAkirin2 in the innate immune system.
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, No. 12, Zhongguancun South Street, Beijing 100081, PR China.
authors.forename: Xianli
authors.initials: X
authors.lastname: Xue
authors.affiliation: National Animal Husbandry Extension Service, Beijing 100125, PR China.
authors.forename: Liwen
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, No. 12, Zhongguancun South Street, Beijing 100081, PR China.
authors.forename: Yeyu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, No. 12, Zhongguancun South Street, Beijing 100081, PR China.
authors.forename: Xinshang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, No. 12, Zhongguancun South Street, Beijing 100081, PR China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Luo
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, No. 12, Zhongguancun South Street, Beijing 100081, PR China.
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, No. 12, Zhongguancun South Street, Beijing 100081, PR China. Electronic address: hengzhao2000@gmail.com.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, No. 12, Zhongguancun South Street, Beijing 100081, PR China. Electronic address: binyao@caas.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Yao
mesh_terms: D016980:Aeromonas hydrophila / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003531:Cypriniformes / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005393:Fish Diseases / Q000276:immunology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000662:veterinary; D007113:Immunity, Innate; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment / Q000662:veterinary
chemical_list: D016207:Cytokines; D029941:Fish Proteins
keywords: Akirin2; Chinese loach (Paramisgurnus dabryanus); Cytokines; Inducible expression; Transcription factor