title: [Sequence analysis of VP1 gene of the duck hepatitis A virus type 3 strains isolated from Shandong Province of China in 2012].
abstract: To reveal the genetic variation of the viral protein 1 (VP1) gene of the duck hepatitis A virus type 3 (DHAV-3), the VP1 gene of 13 virulent DHAV-3 strains isolated from Shandong province of China in 2012 were amplified by RT-PCR, sequenced and analyzed. The results showed that all the VP1 genes of the 13 isolates contained 720 nucleotides encoding 240 amino acids, and shared with nucleotide identities of 94. 6%-99.9% and amino acid identities of 95.0%-100%. The nucleotide and amino acid sequence homologies between the 13 DHAV-3 isolates and other 31 DHAV-3 reference strains were 92.5%-100% and 90. 8%-100%, respectively. Phylogenetic analysis showed that the VP1 gene of DHAV-3 had distinct geographical characteristics. Distribution of genotypes of the 44 DHAV-3 strains was as follows: except the vaccine strain B63, all the other Chinese isolates belonged to genotype I (GI), Vietnamese wild isolates mainly belonged to subtype 1 (S1) of genotype II (GII), and all Korean isolates belonged to subtype 2 (S2) of GII.
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China. sdauxuqian@163.com
authors.forename: Qian
authors.initials: Q
authors.lastname: Xu
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors.forename: Lin-lin
authors.initials: LL
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors.forename: Rui-hua
authors.initials: RH
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors.forename: Zhi-jing
authors.initials: ZJ
authors.lastname: Xie
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors.forename: Yan-li
authors.initials: YL
authors.lastname: Zhu
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors.forename: Shi-jin
authors.initials: SJ
authors.lastname: Jiang
authors.affiliation: Department of Animal Medicine, Foshan Science and Technology University, Foshan 528231, China.
authors.forename: Xing-kui
authors.initials: XK
authors.lastname: Si
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D002681:China; D004372:Ducks; D004371:Hepatitis Virus, Duck / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006524:Hepatitis, Viral, Animal / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000662:veterinary* / Q000821:virology; D011201:Poultry Diseases / Q000821:virology*
chemical_list: D036022:Capsid Proteins