title: Fine mapping of the major Soybean dwarf virus resistance gene Rsdv1 of the soybean cultivar 'Wilis'.
abstract: Soybean dwarf virus (SbDV), a Luteoviridae family member, causes dwarfing, yellowing and sterility of soybean (Glycine max), leading to one of the most serious problems in soybean production in northern Japan. Previous studies revealed that the Indonesian soybean cultivar 'Wilis' is resistant to SbDV and that the resistance can be introduced into Japanese cultivars. A major QTL for SbDV resistance has been reported between SSR markers Sat_217 and Satt211 on chromosome 5. In this study, we named this QTL Rsdv1 (resistance to SbDV) and developed near-isogenic lines incorporating Rsdv1 (Rsdv1-NILs) using Sat_217 and Satt211 markers. The Rsdv1-NILs were resistant to SbDV in greenhouse inoculation and field tests, indicating that Rsdv1 alone is sufficient for the resistance phenotype. We fine-mapped Rsdv1 within the 44-kb region between Sat_11 and Sct_13. None of the six genes predicted in this region was closely related to known virus resistance genes in plants. Thus, Rsdv1 may confer resistance by a previously unknown mechanism. We suggest that Rsdv1 may be a useful source for the Japanese soybean breeding program to introduce SbDV resistance.
authors.affiliation: Hokkaido Research Organization Central Agricultural Experiment Station , Higashi 6 Kita 15, Naganuma, Hokkaido 069-1395 , Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Yamashita
authors.affiliation: Hokkaido Research Organization Central Agricultural Experiment Station , Higashi 6 Kita 15, Naganuma, Hokkaido 069-1395 , Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Takeuchi
authors.affiliation: Hokkaido Research Organization Kitami AES , 52 Yayoi, Kunneppu, Hokkaido 099-1496 , Japan.
authors.forename: Shizen
authors.initials: S
authors.lastname: Ohnishi
authors.affiliation: Hokkaido Research Organization Kitami AES , 52 Yayoi, Kunneppu, Hokkaido 099-1496 , Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Sasaki
authors.affiliation: Hokkaido Research Organization Tokachi AES , S9-2 Shinsei, Memuro, Hokkaido 082-0071 , Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Tazawa
keywords: Glycine max; SSR markers; disease resistance; near-isogenic lines