title: Reduction of organic and inorganic selenium compounds by the edible medicinal basidiomycete Lentinula edodes and the accumulation of elemental selenium nanoparticles in its mycelium.
abstract: We report for the first time that the medicinal basidiomycete Lentinula edodes can reduce selenium from inorganic sodium selenite (Se(IV)) and the organoselenium compound 1,5-diphenyl-3-selenopentanedione-1,5 (DAPS-25) to the elemental state, forming spherical nanoparticles. Submerged cultivation of the fungus with sodium selenite or with DAPS-25 produced an intense red coloration of L. edodes mycelial hyphae, indicating accumulation of elemental selenium (Se(0)) in a red modification. Several methods, including transmission electron microscopy (TEM), electron energy loss spectroscopy (EELS), and X-ray fluorescence, were used to show that red Se(0) accumulated intracellularly in the fungal hyphae as electron-dense nanoparticles with a diameter of 180.51±16.82 nm. Under designated cultivation conditions, shiitake did not reduce selenium from sodium selenate (Se(VI)).
authors.affiliation: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, 410049, Russian Federation.
authors.forename: Elena
authors.initials: E
authors.lastname: Vetchinkina
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Loshchinina
authors.forename: Viktor
authors.initials: V
authors.lastname: Kursky
authors.forename: Valentina
authors.initials: V
authors.lastname: Nikitina
mesh_terms: D046529:Microscopy, Electron, Transmission; D025282:Mycelium / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry; D018036:Selenium Compounds / Q000737:chemistry / Q000378:metabolism*; D020942:Shiitake Mushrooms / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D014675:Vegetables / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D018036:Selenium Compounds