title: slanDCs selectively accumulate in carcinoma-draining lymph nodes and marginate metastatic cells.
abstract: Dendritic cells (DCs) initiate adaptive immune responses to cancer cells by activating naive T lymphocytes. 6-sulfo LacNAc(+) DCs (slanDCs) represent a distinct population of circulating and tissue proinflammatory DCs, whose role in cancer immune surveillance is unknown. Herein, by screening a large set of clinical samples, we demonstrate accumulation of slanDCs in metastatic tumour-draining lymph nodes (M-TDLN) from carcinoma patients. Remarkably, slanDCs are absent at the primary carcinoma site, while their selective nodal recruitment follows the arrival of cancer cells to M-TDLN. slanDCs surround metastatic carcinoma deposits in close proximity to dead cells and efficiently phagocytose tumour cells. In colon carcinoma patients, the contingent of circulating slanDCs remains intact and competent in terms of IL-12p70 and tumour necrosis factor alpha production, induction of T-cell proliferation and migratory capacity to a set of chemokines produced in M-TDLN. We conclude that activated slanDCs represent previously unrecognized players of nodal immune responses to cancer cells.
authors.affiliation: 1] Section of Pathology, Department of Molecular and Translational Medicine, University of Brescia, Brescia 25123, Italy [2] Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri 63110, USA.
authors.forename: William
authors.initials: W
authors.lastname: Vermi
authors.affiliation: 1] Section of General Pathology, Department of Pathology and Diagnostics, University of Verona, Verona 37134, Italy [2].
authors.forename: Alessandra
authors.initials: A
authors.lastname: Micheletti
authors.affiliation: 1] Section of Pathology, Department of Molecular and Translational Medicine, University of Brescia, Brescia 25123, Italy [2].
authors.forename: Silvia
authors.initials: S
authors.lastname: Lonardi
authors.affiliation: Section of General Pathology, Department of Pathology and Diagnostics, University of Verona, Verona 37134, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Costantini
authors.affiliation: Section of General Pathology, Department of Pathology and Diagnostics, University of Verona, Verona 37134, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Calzetti
authors.affiliation: Section of Pathology, Department of Molecular and Translational Medicine, University of Brescia, Brescia 25123, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Nascimbeni
authors.affiliation: Section of Pathology, Department of Molecular and Translational Medicine, University of Brescia, Brescia 25123, Italy.
authors.forename: Mattia
authors.initials: M
authors.lastname: Bugatti
authors.affiliation: Section of Pathology, Department of Molecular and Translational Medicine, University of Brescia, Brescia 25123, Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Codazzi
authors.affiliation: Section of Otorhinolaryngology, Department of Surgery, University of Verona, Verona 37134, Italy.
authors.forename: Patrick C
authors.initials: PC
authors.lastname: Pinter
authors.affiliation: Department of Dermatology, University Hospital Heidelberg, 69115 Heidelberg, Germany.
authors.forename: Knut
authors.initials: K
authors.lastname: Schäkel
authors.affiliation: Section of General Pathology, Department of Pathology and Diagnostics, University of Verona, Verona 37134, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Tamassia
authors.affiliation: Section of General Pathology, Department of Pathology and Diagnostics, University of Verona, Verona 37134, Italy.
authors.forename: Marco A
authors.initials: MA
authors.lastname: Cassatella
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000606:Amino Sugars / Q000378:metabolism; D002277:Carcinoma / Q000276:immunology* / Q000556:secondary; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006801:Humans; D018664:Interleukin-12 / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology* / Q000473:pathology; D008207:Lymphatic Metastasis; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000276:immunology* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: C467438:6-sulfo-LacNac; D000606:Amino Sugars; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12