title: [Protective effect of Pinus massoniana bark extracts against cisplatin-induced nephrotoxicity in human embryonic kidney cells].
abstract: OBJECTIVE
  To investigate the in vitro protective effect of Pinus massoniana bark extracts (PMBE) against cisplatin-induced nephrotoxicity in human embryonic kidney cells (HEK293), and preliminarily study its mechanism.
  
  
  METHODS
  Human embryonic kidney cells (HEK293) were cultured in vitro. The MTT assay was adopted to test the effect of PMBE and cisplatin on growth of HEK293 cells, and the protective effect of PMBE on cisplatin-induced nephrotoxicity of HEK293, and then detect the intracellular reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH) content, catalase (CAT), superoxide dismutase (SOD) and activity of thioredoxin reductase (TrxR).
  
  
  RESULTS
  PMBE could promote growth of HEK293 cells at low concentrations, but generate slight nephrotoxicity at high concentration. Cisplatin could inhibit growth of HEK293 cells, increase ROS and MDA content, while reducing SOD, CAT and TrxR. The pre-protective PMBE was added to reduce cisplatin's injury to HEK293 cells, ROS, MDA and GSH content, SOD, CAT and TrxR within certain range.
  
  
  CONCLUSIONS
  PMBE at specific concentration has the protective effect in cisplatin-induced nephrotoxicity in HEK293 cells. Its mechanism may be related to PMBE's antioxidant activity.
authors.affiliation: State Key Laboratory for Pest control and Resource Utilization, College of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. lssfdr@mail.sysu.edu.cn
authors.forename: Dong-Ru
authors.initials: DR
authors.lastname: Feng
authors.affiliation: State Key Laboratory for Pest control and Resource Utilization, College of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Qing-Chen
authors.initials: QC
authors.lastname: Dai
authors.affiliation: Guangdong Songzhen Nutrition Source Research Institute, Guangzhou 510645, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Xie
authors.affiliation: Guangdong Songzhen Nutrition Source Research Institute, Guangzhou 510645, China.
authors.forename: Ruo-Da
authors.initials: RD
authors.lastname: Li
authors.affiliation: Guangdong Songzhen Nutrition Source Research Institute, Guangzhou 510645, China.
authors.forename: Guang-Yao
authors.initials: GY
authors.lastname: Zheng
authors.affiliation: State Key Laboratory for Pest control and Resource Utilization, College of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Jin-Fa
authors.initials: JF
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Pest control and Resource Utilization, College of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002945:Cisplatin / Q000633:toxicity*; D004847:Epithelial Cells / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D028223:Pinus / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D020011:Protective Agents; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D013880:Thioredoxin-Disulfide Reductase; D005978:Glutathione; D002945:Cisplatin