title: The PURE system for protein production.
abstract: In the field of molecular biology or biochemistry, preparation and use of purified proteins involved in a certain biological system is crucial for understanding their mechanisms and functions in cells or organisms. The recent progress in a cell-free translation system allows us to prepare proteins in a test tube directly from cDNAs that encode the amino acid sequences. The use of the reconstituted cell-free translation system termed PURE (Protein synthesis Using Recombinant Elements) for these purposes is effective in several applications. Here we describe methods of recombinant protein expression using the PURE system for molecular biological or biochemical studies.
authors.affiliation: Laboratory for Cell-Free Protein Synthesis, Quantitative Biology Center, RIKEN, Chuo-ku, Kobe, Hyogo, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Yutetsu
authors.initials: Y
authors.lastname: Kuruma
authors.forename: Takashi
authors.initials: T
authors.lastname: Kanamori
authors.forename: Takuya
authors.initials: T
authors.lastname: Ueda
mesh_terms: D001483:Base Sequence; D002462:Cell Membrane / Q000378:metabolism; D002474:Cell-Free System; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004220:Disulfides; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins