title: Application of UPLC-QTOF-MS in MS(E) mode for the rapid and precise identification of alkaloids in goldenseal (Hydrastis canadensis).
abstract: Here, we describe a new application of ultra-performance liquid chromatography coupled with an electrospray ionization quadrupole time-of-flight mass spectrometry operating in MS(E) mode (UPLC-QTOF-MS(E)) for the sensitive, fast, and effective characterization of alkaloids in goldenseal (Hydrastis canadensis). This approach allowed identification of alkaloids using a cyclic low and high collision energy spectral acquisition mode providing simultaneous accurate precursor and fragment ion mass information. A total of 45 compounds were separated and 40 of them characterized including one new compound and 7 identified for the first time in goldenseal. The spectral data obtained using this method is comparable to those obtained by conventional LC-MS(n). However, the UPLC-QTOF-MS(E) method offers high chromatographic resolution with structural characterization facilitated by accurate mass measurement in both MS and MS/MS modes in a single analytical run; this makes it suitable for the rapid analysis and screening of alkaloids in plant extracts.
authors.affiliation: Measurement Science and Standards, National Research Council Canada, 1200 Montreal Road, Ottawa, ON, K1A 0R6, Canada, PhuongMai.Le@nrc-cnrc.gc.ca.
authors.forename: Phuong Mai
authors.initials: PM
authors.lastname: Le
authors.forename: Margaret
authors.initials: M
authors.lastname: McCooeye
authors.forename: Anthony
authors.initials: A
authors.lastname: Windust
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods; D039321:Hydrastis / Q000737:chemistry*; D013058:Mass Spectrometry / Q000191:economics / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry*; D013997:Time Factors
chemical_list: D000470:Alkaloids; D010936:Plant Extracts