title: A microfabricated, optically accessible device to study the effects of mechanical cues on collagen fiber organization.
abstract: As the primary structural protein of our bodies, fibrillar collagen and its organizational patterns determine the biomechanics and shape of tissues. While the molecular assembly of individual fibrils is well understood, the mechanisms determining the arrangement of fibers and thus the shape and form of tissues remain largely unknown. We have developed a cell culture model that successfully recapitulates early tissue development and the de novo deposition of collagen fibers to investigate the role of mechanical cues on collagen fiber alignment. The devices used a thin, collagen-coated deformable PDMS membrane inside a tissue culture well built on microscope-grade coverslips. Deformations and strains in the PDMS membrane were quantified by tracking fluorescent bead displacement and through the use of a COMSOL model. Cyclical strains were applied to serum-cultured rabbit corneal cells at 0.5 Hz for 24-48 h and showed a preferred alignment after 36 h of cyclical loading. Cells cultured with ascorbic acid under methylcellulose serum-free conditions deposited a collagenous matrix that was visible under live second harmonic generation microscopy at week 4. Our microfabricated tissue culture system allows for the controllable application of a wide range of stress profiles to cells, and for the observation and quantification of cells and de novo collagen formation in vitro. Future studies will involve the fabrication of models to study the formation and organization of collagen in ocular diseases.
authors.affiliation: Department of Biomedical Engineering, 3120 Natural Sciences II, University of California, Irvine, CA, 92697, USA.
authors.forename: Moritz
authors.initials: M
authors.lastname: Winkler
authors.forename: Melinda G
authors.initials: MG
authors.lastname: Simon
authors.forename: Timothy
authors.initials: T
authors.lastname: Vu
authors.forename: Trevor L
authors.initials: TL
authors.lastname: Gartner
authors.forename: James V
authors.initials: JV
authors.lastname: Jester
authors.forename: Abraham P
authors.initials: AP
authors.lastname: Lee
authors.forename: Donald J
authors.initials: DJ
authors.lastname: Brown
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D003315:Cornea / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D008962:Models, Theoretical; D009757:Nylons / Q000737:chemistry; D011817:Rabbits
chemical_list: D004129:Dimethylpolysiloxanes; D009757:Nylons; C085707:poly(dimethylsiloxane)-polyamide copolymer; D003094:Collagen