title: A molecular insight into the role of inflammation in the behavior and pathogenesis of odontogenic cysts.
abstract: BACKGROUND
  Although, odontogenickeratocyst (OKC) and dentigerous cyst (DC) are considered asdevelopmental cysts inflammation has been seen in its connective tissue wall. Inflammation is seen to alter the epithelial lining of both OKC and DC butwhether it plays in altering the behavior of these cysts is not fully understood.
  
  
  OBJECTIVE
  The present study is conducted with the help of molecular marker proliferating cellular nuclear antigen (PCNA) to assess the proliferative activity of OKC and DC and to further evaluate and correlate the effect of inflammation on the proliferative activity and hence on biological behavior of these cysts.
  
  
  METHODS
  Immunohistochemicalstaining was performed using anti-PCNA antibody in 10 cases each of classical OKC, inflamed OKC, classical DC, and inflamed DC. The resulting data was tabulated on Microsoft excel and subjected to statistical analysis using two-way analysis of variance test, t test and post-hoc test followed by Bonferroni test with the application of statistical package for social sciences (SPSS) (SPSS, Chicago, IL, USA) and Epi-Info 6.04 d, Atlanta, Georgia (USA).
  
  
  RESULTS
  Total mean PCNA expression is statistically higher in inflamed OKC than classical OKC and inflamed DC also showed significantly higher PCNA expression than classical DC (P < 0.001). Correlation between inflammation and PCNA expression was not statistically significant (P ≥ 0.05).
  
  
  CONCLUSIONS
  Inflammation is responsible for change in behavior of neoplastic epithelium of OKC, whereas in dentigerous it is responsible for changes in epithelial lining and hence should be treated meticulously.
authors.affiliation: Department of Oral and Maxillofacial Pathology and Microbiology, Dasmesh Institute of Research and Dental Sciences, Faridkot, Punjab, India.
authors.forename: Hp
authors.initials: H
authors.lastname: Singh
authors.affiliation: Department of Oral and Maxillofacial Pathology and Microbiology, ITS Dental College, Ghaziabad, Uttar Pradesh, India.
authors.forename: Dc
authors.initials: D
authors.lastname: Shetty
authors.affiliation: Department of Oral and Maxillofacial Pathology and Microbiology, Yamuna Institute of Dental Sciences and Research, Yammunanagar, Haryana, India.
authors.forename: A
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Periodontics, Rungta College of Dental Sciences and Research, Bhillai, Chattisgarh, India.
authors.forename: R
authors.initials: R
authors.lastname: Chavan
authors.affiliation: Department of Conservative Dentistry and Endodontics, VSPM Dental College and Research Center, Nagpur, India.
authors.forename: Dd
authors.initials: D
authors.lastname: Shori
authors.affiliation: Department of Periodontics, Vaidik Dental College and Research Center, Daman, India.
authors.forename: J
authors.initials: J
authors.lastname: Mali
keywords: Cyst pathogenesis; Dentigerouscyst; Odontogeniccyst; Odontogenickeratocyst; Proliferatingcellular nuclear antigen