title: Correlation of Aggregatibacter actinomycetemcomitans detection with clinical/immunoinflammatory profile of localized aggressive periodontitis using a 16S rRNA microarray method: a cross-sectional study.
abstract: OBJECTIVE
  The objective of this study was to determine whether the detection of Aggregatibacter actinomycetemcomitans (Aa) correlates with the clinical and immunoinflammatory profile of Localized Aggressive Periodontitis (LAP), as determined by by 16S rRNA gene-based microarray.
  
  
  METHODS
  Subgingival plaque samples from the deepest diseased site of 30 LAP patients [PD ≥ 5 mm, BoP and bone loss] were analyzed by 16S rRNA gene-based microarrays. Gingival crevicular fluid (GCF) samples were analyzed for 14 cyto/chemokines. Peripheral blood was obtained and stimulated in vitro with P.gingivalis and E.coli to evaluate inflammatory response profiles. Plasma lipopolysaccharide (LPS) levels were also measured.
  
  
  RESULTS
  Aa was detected in 56% of LAP patients and was shown to be an indicator for different bacterial community structures (p<0.01). Elevated levels of pro-inflammatory cyto/chemokines were detected in LPS-stimulated blood samples in both Aa-detected and Aa-non-detected groups (p>0.05). Clinical parameters and serum LPS levels were similar between groups. However, Aa-non-detected GCF contained higher concentration of IL-8 than Aa-detected sites (p<0.05). TNFα and IL1β were elevated upon E.coli LPS stimulation of peripheral blood cells derived from patients with Aa-detected sites.
  
  
  CONCLUSIONS
  Our findings demonstrate that the detection of Aa in LAP affected sites, did not correlate with clinical severity of the disease at the time of sampling in this cross-sectional study, although it did associate with lower local levels of IL-8, a different subgingival bacterial profile and elevated LPS-induced levels of TNFα and IL1β.
authors.affiliation: Department of Dentistry, Federal University of Jequitinhonha and Mucuri Valleys, Diamantina, Minas Gerais, Brazil ; Department of Periodontology, University of Florida College of Dentistry, Gainesville, Florida, United States of America.
authors.forename: Patricia F
authors.initials: PF
authors.lastname: Gonçalves
authors.affiliation: Department of Microbial Ecology and Pathogenesis, The Fortsyth Institute, Cambridge, Massachusetts, United States of America ; Department of Biological Sciences, Wellesley College, Wellesley, Massachusetts, United States of America.
authors.forename: Vanja
authors.initials: V
authors.lastname: Klepac-Ceraj
authors.affiliation: Department of Periodontology, University of Florida College of Dentistry, Gainesville, Florida, United States of America.
authors.forename: Hong
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Microbial Ecology and Pathogenesis, The Fortsyth Institute, Cambridge, Massachusetts, United States of America ; Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, Boston, Massachusetts, United States of America.
authors.forename: Bruce J
authors.initials: BJ
authors.lastname: Paster
authors.affiliation: Department of Periodontology, University of Florida College of Dentistry, Gainesville, Florida, United States of America.
authors.forename: Ikramuddin
authors.initials: I
authors.lastname: Aukhil
authors.affiliation: Department of Periodontology, University of Florida College of Dentistry, Gainesville, Florida, United States of America.
authors.forename: Shannon M
authors.initials: SM
authors.lastname: Wallet
authors.affiliation: Department of Periodontology, University of Florida College of Dentistry, Gainesville, Florida, United States of America.
authors.forename: Luciana M
authors.initials: LM
authors.lastname: Shaddox
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics*; D010520:Aggressive Periodontitis / Q000235:genetics*; D001938:Brazil; D003430:Cross-Sectional Studies; D016207:Cytokines / Q000032:analysis; D005883:Gingival Crevicular Fluid / Q000737:chemistry; D006801:Humans; D008070:Lipopolysaccharides / Q000097:blood; D046228:Microarray Analysis / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D012336:RNA, Ribosomal, 16S