title: Comparative high-resolution mapping of the wax inhibitors Iw1 and Iw2 in hexaploid wheat.
abstract: The wax (glaucousness) on wheat leaves and stems is mainly controlled by two sets of genes: glaucousness loci (W1 and W2) and non-glaucousness loci (Iw1 and Iw2). The non-glaucousness (Iw) loci act as inhibitors of the glaucousness loci (W). High-resolution comparative genetic linkage maps of the wax inhibitors Iw1 originating from Triticum dicoccoides, and Iw2 from Aegilops tauschii were developed by comparative genomics analyses of Brachypodium, sorghum and rice genomic sequences corresponding to the syntenic regions of the Iw loci in wheat. Eleven Iw1 and eight Iw2 linked EST markers were developed and mapped to linkage maps on the distal regions of chromosomes 2BS and 2DS, respectively. The Iw1 locus mapped within a 0.96 cM interval flanked by the BE498358 and CA499581 EST markers that are collinear with 122 kb, 202 kb, and 466 kb genomic regions in the Brachypodium 5S chromosome, the sorghum 6S chromosome and the rice 4S chromosome, respectively. The Iw2 locus was located in a 4.1 to 5.4-cM interval in chromosome 2DS that is flanked by the CJ886319 and CJ519831 EST markers, and this region is collinear with a 2.3 cM region spanning the Iw1 locus on chromosome 2BS. Both Iw1 and Iw2 co-segregated with the BF474014 and CJ876545 EST markers, indicating they are most likely orthologs on 2BS and 2DS. These high-resolution maps can serve as a framework for chromosome landing, physical mapping and map-based cloning of the wax inhibitors in wheat.
authors.affiliation: State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Wu
authors.affiliation: State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.
authors.forename: Jinxia
authors.initials: J
authors.lastname: Qin
authors.affiliation: State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China ; Plant Science and Technology College, Beijing University of Agriculture, Beijing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Han
authors.affiliation: State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Ouyang
authors.affiliation: State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
authors.affiliation: State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.
authors.forename: Jingzhong
authors.initials: J
authors.lastname: Xie
authors.affiliation: State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Cui
authors.affiliation: State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.
authors.forename: Huiru
authors.initials: H
authors.lastname: Peng
authors.affiliation: State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.
authors.forename: Qixin
authors.initials: Q
authors.lastname: Sun
authors.affiliation: State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Liu
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D020224:Expressed Sequence Tags; D005819:Genetic Markers / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D013045:Species Specificity; D014908:Triticum / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D010940:Plant Proteins