title: Subtilase SprP exerts pleiotropic effects in Pseudomonas aeruginosa.
abstract: The open reading frame PA1242 in the genome of Pseudomonas aeruginosa PAO1 encodes a putative protease belonging to the peptidase S8 family of subtilases. The respective enzyme termed SprP consists of an N-terminal signal peptide and a so-called S8 domain linked by a domain of unknown function (DUF). Presumably, this DUF domain defines a discrete class of Pseudomonas proteins as homologous domains can be identified almost exclusively in proteins of the genus Pseudomonas. The sprP gene was expressed in Escherichia coli and proteolytic activity was demonstrated. A P. aeruginosa ∆sprP mutant was constructed and its gene expression pattern compared to the wild-type strain by genome microarray analysis revealing altered expression levels of 218 genes. Apparently, SprP is involved in regulation of a variety of different cellular processes in P. aeruginosa including pyoverdine synthesis, denitrification, the formation of cell aggregates, and of biofilms.
authors.affiliation: Institute of Molecular Enzyme Technology, Research Centre Juelich, Heinrich-Heine-University Duesseldorf, D-52426, Juelich, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Pelzer
authors.forename: Tino
authors.initials: T
authors.lastname: Polen
authors.forename: Horst
authors.initials: H
authors.lastname: Funken
authors.forename: Frank
authors.initials: F
authors.lastname: Rosenau
authors.forename: Susanne
authors.initials: S
authors.lastname: Wilhelm
authors.forename: Michael
authors.initials: M
authors.lastname: Bott
authors.forename: Karl-Erich
authors.initials: KE
authors.lastname: Jaeger
mesh_terms: D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D018441:Biofilms; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D058440:Denitrification; D004926:Escherichia coli; D017353:Gene Deletion; D005798:Genes, Bacterial; D058685:Genetic Pleiotropy; D009842:Oligopeptides / Q000096:biosynthesis; D016366:Open Reading Frames; D017434:Protein Structure, Tertiary; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity; D012329:RNA, Bacterial / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013381:Subtilisins / Q000235:genetics / Q000502:physiology*; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D009842:Oligopeptides; D012329:RNA, Bacterial; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; C042453:pyoverdin; D013381:Subtilisins
keywords: Biofilm; Pseudomonas aeruginosa; Pyoverdine.; microarray; motility; orf PA1242; protease