title: Anti-tissue transglutaminase antibody inhibits apoptotic cell clearance by macrophages in pregnant NOD mice.
abstract: Autoimmunity is a feature of celiac disease (CD) with tissue transglutaminase (tTG) as a major autoantigen. A correlation between gynecological-obstetric disorders in CD patients and the presence of circulating antibodies anti-tTG that inhibited tTG activity was reported. Serum anti-tTG antibodies were detected in a non-obese diabetic (NOD) mouse model of type I insulin-dependent diabetes mellitus and Sjögren's syndrome, two comorbid states with CD. Since pregnancy complications have been described in NOD mice, we evaluated the ability of anti-tTG antibodies to affect the functions of tTG relevant to the normal course of an early pregnancy like extracellular matrix assembling and apoptotic cell phagocytosis by macrophages. Circulating IgG antibodies against tTG were detected in NOD mice with titers that decreased at early pregnancy; interestingly, the in vitro transamidating activity of tTG was reduced by NOD serum samples. Particularly, anti-tTG antibody inhibited apoptotic cell phagocytosis by peritoneal macrophages from pregnant NOD mice that express the enzyme on surface. Evidence provided support for a role for anti-tTG antibodies through reduced transamidating activity and reduced apoptotic cell clearance by the macrophages of pregnant NOD mice.
authors.affiliation: Cátedra de Inmunología, Facultad de Ciencias/Facultad de Química, Universidad de la República, Montevideo, Uruguay; Escuela Universitaria de Tecnología Médica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay. Electronic address: csonora@fmed.edu.uy.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Sóñora
authors.affiliation: Cátedra de Inmunología, Facultad de Ciencias/Facultad de Química, Universidad de la República, Montevideo, Uruguay.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Mourglia-Ettlin
authors.affiliation: Laboratorio de Inmunofarmacología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN-CONICET, Buenos Aires, Argentina.
authors.forename: Guillermina
authors.initials: G
authors.lastname: Calo
authors.affiliation: Laboratorio de Inmunofarmacología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN-CONICET, Buenos Aires, Argentina.
authors.forename: Vanesa
authors.initials: V
authors.lastname: Hauk
authors.affiliation: Laboratorio de Inmunofarmacología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN-CONICET, Buenos Aires, Argentina.
authors.forename: Rosanna
authors.initials: R
authors.lastname: Ramhorst
authors.affiliation: Cátedra de Inmunología, Facultad de Ciencias/Facultad de Química, Universidad de la República, Montevideo, Uruguay.
authors.forename: Ana
authors.initials: A
authors.lastname: Hernández
authors.affiliation: Laboratorio de Inmunofarmacología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN-CONICET, Buenos Aires, Argentina.
authors.forename: Claudia Pérez
authors.initials: CP
authors.lastname: Leirós
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology; D017209:Apoptosis / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology; D002446:Celiac Disease / Q000276:immunology*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000276:immunology; D005260:Female; D019204:GTP-Binding Proteins / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016688:Mice, Inbred NOD; D010587:Phagocytosis / Q000276:immunology*; D011247:Pregnancy; D011256:Pregnancy Outcome; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D012859:Sjogren's Syndrome / Q000097:blood / Q000276:immunology; D011503:Transglutaminases / Q000276:immunology*; D014599:Uterus / Q000276:immunology
chemical_list: D000906:Antibodies; D001324:Autoantigens; D007074:Immunoglobulin G; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins
keywords: Anti-tissue transglutaminase antibodies; Celiac disease; NOD mice