title: An N-terminal splice variant of human Stat5a that interacts with different transcription factors is the dominant form expressed in invasive ductal carcinoma.
abstract: We have identified a new variant of human Stat5a, found at higher ratios to full-length Stat5a in invasive ductal carcinoma versus contiguous normal tissue. The variant, missing exon 5, inhibits p21 and Bax production and increases cell number. After prolactin stimulation, only full-length Stat5a interacts with the vitamin D and retinoid X receptors, whereas only Δ5 Stat5a interacts with activating protein 1-2 and specificity protein 1. Prolactin also oppositely regulates interaction of the two Stat5a forms with β-catenin. We propose that a change in splicing leading to upregulation of this new isoform is a pathogenic aspect of invasive ductal carcinoma.
authors.affiliation: Division of Biomedical Sciences, University of California, Riverside, Riverside, CA 92521-0121, United States; College of Medicine/Institute of Medical Sciences, Jishou University, Jishou 416000, Hunan, PR China.
authors.forename: Dunyong
authors.initials: D
authors.lastname: Tan
authors.affiliation: Division of Biomedical Sciences, University of California, Riverside, Riverside, CA 92521-0121, United States.
authors.forename: KuanHui E
authors.initials: KE
authors.lastname: Chen
authors.affiliation: Division of Biomedical Sciences, University of California, Riverside, Riverside, CA 92521-0121, United States; Department of Biochemistry, University of California, Riverside, Riverside, CA 92521-0121, United States.
authors.forename: Changhui
authors.initials: C
authors.lastname: Deng
authors.affiliation: People's Hospital of Xiangxi Autonomous Region, Jishou University, Jishou 416000, Hunan, PR China.
authors.forename: Peizhi
authors.initials: P
authors.lastname: Tang
authors.affiliation: People's Hospital of Xiangxi Autonomous Region, Jishou University, Jishou 416000, Hunan, PR China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Huang
authors.affiliation: Division of Biomedical Sciences, University of California, Riverside, Riverside, CA 92521-0121, United States.
authors.forename: Trina
authors.initials: T
authors.lastname: Mansour
authors.affiliation: Division of Biomedical Sciences, University of California, Riverside, Riverside, CA 92521-0121, United States; Department of Biochemistry, University of California, Riverside, Riverside, CA 92521-0121, United States.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Luben
authors.affiliation: Division of Biomedical Sciences, University of California, Riverside, Riverside, CA 92521-0121, United States. Electronic address: ameae.walker@ucr.edu.
authors.forename: Ameae M
authors.initials: AM
authors.lastname: Walker
mesh_terms: D001483:Base Sequence; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D018270:Carcinoma, Ductal, Breast / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation; D006801:Humans; D008969:Molecular Sequence Data; D020033:Protein Isoforms; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050799:STAT5 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D020033:Protein Isoforms; D050799:STAT5 Transcription Factor; C494092:STAT5A protein, human; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins
keywords: Breast cancer; Interaction with other signaling/transcription factors; Invasive ductal carcinoma; Splice variant; Stat5a