title: Association of duffy blood group gene polymorphisms with IL8 gene in chronic periodontitis.
abstract: The antigens of the Duffy blood group system (DARC) act as a receptor for the interleukin IL-8. IL-8 plays an important role in the pathogenesis of chronic periodontitis due to its chemotactic properties on neutrophils. The aim of this study was to investigate a possible association of Duffy blood group gene polymorphisms with the -353T>A, -845T>C and -738T>A SNPs of the IL8 gene in chronic periodontitis. One hundred and twenty-four individuals with chronic periodontitis and 187 controls were enrolled. DNA was extracted using the salting-out method. The Duffy genotypes and IL8 gene promoter polymorphisms were investigated by PCR-RFLP. Statistical analyses were conducted using the Chi square test with Yates correction or Fisher's Exact Test, and the possibility of associations were evaluated by odds ratio with a 95% confidence interval. When analyzed separately, for the Duffy blood group system, differences in the genotype and allele frequencies were not observed between all the groups analyzed; and, in nonsmokers, the -845C allele (3.6% vs. 0.4%), -845TC genotype (7.3% vs. 0.7%) and the CTA haplotype (3.6% vs. 0.4%) were positively associated with chronic periodontitis. For the first time to our knowledge, the polymorphisms of erythroid DARC plus IL8 -353T>A SNPs were associated with chronic periodontitis in Brazilian individuals. In Afro-Brazilians patients, the FY*02N.01 with IL8 -353A SNP was associated with protection to chronic periodontitis.
authors.affiliation: Post Graduation Program of Biosciences Applied to Pharmacy, Department of Analysis Clinical and Biomedicine, Maringa State University, Parana, Brazil.
authors.forename: Emília Ângela
authors.initials: EÂ
authors.lastname: Sippert
authors.affiliation: Dentistry Department, Maringa State University, Parana, Brazil.
authors.forename: Cléverson
authors.initials: C
authors.lastname: de Oliveira e Silva
authors.affiliation: Basic Health Sciences Department, Maringa State University, Parana, Brazil.
authors.forename: Jeane Eliete Laguila
authors.initials: JE
authors.lastname: Visentainer
authors.affiliation: Basic Health Sciences Department, Maringa State University, Parana, Brazil.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Sell
mesh_terms: D001938:Brazil; D004375:Duffy Blood-Group System / Q000235:genetics*; D005787:Gene Frequency; D056726:Genetic Association Studies; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D016017:Odds Ratio; D010518:Periodontitis / Q000235:genetics*; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic
chemical_list: C508606:CXCL8 protein, human; D004375:Duffy Blood-Group System; D016209:Interleukin-8