title: Polylactide nanofibers with hydroxyapatite as growth substrates for osteoblast-like cells.
abstract: Various types of nanofibers are increasingly used in tissue engineering, mainly for their ability to mimic the architecture of tissue at the nanoscale. We evaluated the adhesion, growth, viability, and differentiation of human osteoblast-like MG 63 cells on polylactide (PLA) nanofibers prepared by needle-less electrospinning and loaded with 5 or 15 wt % of hydroxyapatite (HA) nanoparticles. On day 7 after seeding, the cell number was the highest on samples with 15 wt % of HA. This result was confirmed by the XTT test, especially after dynamic cultivation, when the number of metabolically active cells on these samples was even higher than on control polystyrene. Staining with a live/dead kit showed that the viability of cells on all nanofibrous scaffolds was very high and comparable to that on control polystyrene dishes. An enzyme-linked immunosorbent assay revealed that the concentration of osteocalcin was also higher in cells on samples with 15 wt % of HA. There was no immune activation of cells (measured by production of TNF-alpha), associated with the incorporation of HA. Moreover, the addition of HA suppressed the creep behavior of the scaffolds in their dry state. Thus, nanofibrous PLA scaffolds have potential for bone tissue engineering, particularly those with 15 wt % of HA.
authors.affiliation: Department of Biomaterials and Tissue Engineering Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska, 1083, 14220 Prague 4, Czech Republic.
authors.forename: Katarina
authors.initials: K
authors.lastname: Novotna
authors.forename: Martina
authors.initials: M
authors.lastname: Zajdlova
authors.forename: Tomas
authors.initials: T
authors.lastname: Suchy
authors.forename: Daniel
authors.initials: D
authors.lastname: Hadraba
authors.forename: Frantisek
authors.initials: F
authors.lastname: Lopot
authors.forename: Margit
authors.initials: M
authors.lastname: Zaloudkova
authors.forename: Timothy E L
authors.initials: TE
authors.lastname: Douglas
authors.forename: Marcela
authors.initials: M
authors.lastname: Munzarova
authors.forename: Martina
authors.initials: M
authors.lastname: Juklickova
authors.forename: Denisa
authors.initials: D
authors.lastname: Stranska
authors.forename: Dana
authors.initials: D
authors.lastname: Kubies
authors.forename: David
authors.initials: D
authors.lastname: Schaubroeck
authors.forename: Sebastian
authors.initials: S
authors.lastname: Wille
authors.forename: Lieve
authors.initials: L
authors.lastname: Balcaen
authors.forename: Marketa
authors.initials: M
authors.lastname: Jarosova
authors.forename: Halyna
authors.initials: H
authors.lastname: Kozak
authors.forename: Alexander
authors.initials: A
authors.lastname: Kromka
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Svindrych
authors.forename: Vera
authors.initials: V
authors.lastname: Lisa
authors.forename: Karel
authors.initials: K
authors.lastname: Balik
authors.forename: Lucie
authors.initials: L
authors.lastname: Bacakova
mesh_terms: D018786:Bone Substitutes; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D002470:Cell Survival; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D057139:Nanofibers / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D015675:Osteocalcin / Q000096:biosynthesis; D011091:Polyesters / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods
chemical_list: D018786:Bone Substitutes; D011091:Polyesters; D015675:Osteocalcin; C033616:poly(lactide); D017886:Durapatite
keywords: electrospinning; hydroxyapatite; nanofibers; polylactide; tissue engineering