title: Study of bilineage differentiation of human-bone-marrow-derived mesenchymal stem cells in oxidized sodium alginate/N-succinyl chitosan hydrogels and synergistic effects of RGD modification and low-intensity pulsed ultrasound.
abstract: The level of formation of new bone and vascularization in bone tissue engineering scaffold implants is considered as a critical factor for clinical application. In this study, an approach using an RGD-grafted oxidized sodium alginate/N-succinyl chitosan (RGD-OSA/NSC) hydrogel as a scaffold and low-intensity pulsed ultrasound (LIPUS) as mechanical stimulation was proposed to achieve a high level of formation of new bone and vascularization. An in vitro study of endothelial and osteogenic differentiations of human-bone-marrow-derived mesenchymal stem cells (hMSCs) was conducted to evaluate it. The results showed that RGD-OSA/NSC composite hydrogels presented good biological properties in attachment, proliferation and differentiation of cells. The MTT cell viability assay showed that the total number of cells increased more significantly in the LIPUS-stimulated groups with RGD than that in the control ones; similar results were obtained for alkaline phosphatase activity/staining and mineralized nodule formation assay of osteogenic induction and immunohistochemical test of endothelial induction. The positive synergistic effect of LIPUS and RGD on the enhancement of proliferation and differentiation of hMSCs was observed. These findings suggest that the hybrid use of RGD modification and LIPUS might provide one approach to achieve a high level of formation of new bone and vascularization in bone tissue engineering scaffold implants.
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, People's Republic of China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, People's Republic of China.
authors.forename: Wenzhen
authors.initials: W
authors.lastname: Peng
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.affiliation: Sichuan Centre for Disease Control and Prevention, Chengdu 610041, People's Republic of China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Sichuan Centre for Disease Control and Prevention, Chengdu 610041, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.affiliation: Sichuan Centre for Disease Control and Prevention, Chengdu 610041, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Peng
authors.affiliation: Sichuan Centre for Disease Control and Prevention, Chengdu 610041, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Feng
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhi
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Weng
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, People's Republic of China. Electronic address: j.wang63@gmail.com.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Wang
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D020100:Hydrogels; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009842:Oligopeptides / Q000737:chemistry*; D010084:Oxidation-Reduction; D014465:Ultrasonics
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020100:Hydrogels; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D020723:Glucuronic Acid; D048271:Chitosan
keywords: Bone tissue engineering; Endothelial differentiation; Hydrogel; LIPUS; Osteogenic differentiation