title: Characteristic behavior of crowding macromolecules confined in cell-sized droplets.
abstract: We describe the unique behavior of long DNA molecules in a cell-sized confinement by using cell-sized water-in-oil droplets coated with a lipid layer. It is shown that DNA molecules exhibit specific localization on lipid membranes accompanied by conformational transitions depending on the spatial size of droplets as well as the chemical structure of lipids coating the droplets. Interestingly, such specific behavior of DNA molecules causes on/off switching of transcriptional activity. We demonstrate the effect of crowding by adapting various kinds of macromolecules with different electronic charges and flexibility. As recently reported, it is shown that gene expression is remarkably accelerated in cell-sized confinement. We theoretically discuss the abovementioned unique behavior of DNA molecules in a micro-confinement. Further study in comparison with the actual behavior of DNA molecules in a living cellular system is expected to be fruitful.
authors.affiliation: Department of Physics, Graduate School of Sciences, Kyushu University, Fukuoka, Japan. Electronic address: keyoshik@mail.doshisha.ac.jp.
authors.forename: Miho
authors.initials: M
authors.lastname: Yanagisawa
authors.affiliation: Department of Physics, Graduate School of Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Sakaue
authors.affiliation: Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Kyoto, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yoshikawa
mesh_terms: D000818:Animals; D002474:Cell-Free System / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry*; D014158:Transcription, Genetic
chemical_list: D008051:Lipid Bilayers; D004247:DNA
keywords: Cell-sized confinement; DNA structure and function; Depletion effect; Micro-thermodynamics; Microphase separation; Transcription