title: Evaluation of hepatotoxicity and clastogenicity of carbofuran in male Wistar rats.
abstract: Carbofuran based pesticides have gained wide usage in Nigeria recently. Consequently, animals and human populations are exposed to them in the environment. Information on in vivo toxicity of carbofuran in experimental models is scanty. The present study therefore examined the hepatotoxicity and clastogenic effects of carbofuran in rats. Male Wistar rats were exposed to carbofuran (p.o) at 0-5mg/kg bw for 5weeks. Carbofuran induced significant (p<0.05) increase in the serum activity of gamma-glutamyltransferase when compared with the negative control, but not activity of serum alanine and aspartate aminotransferases. It also significantly (p<0.05) induced micronucleated polychromatic erythrocytes formation in the bone marrow as compared with the control. The level of induction is dose dependent in both cases. In addition, there was significant (p<0.05) higher number of hepatic cells in the cell/mm(2) assay for the group treated with carbofuran. Histopathological analysis of liver samples from the treated groups revealed lesions ranging from general congestion (portal, central venous and sinusoidal), mild periportal cellular infiltration, diffused sinusoidal congestion and hepatic necrosis to severe congestion. Findings from this study suggest that carbofuran has clastogenic and hepatotoxic effects in rats. It therefore may constitute an environmental health risks in individuals so exposed.
authors.affiliation: Cancer Research and Molecular Biology Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria. Electronic address: magbadegesin@yahoo.com.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Gbadegesin
authors.affiliation: Cancer Research and Molecular Biology Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria.
authors.forename: Solomon E
authors.initials: SE
authors.lastname: Owumi
authors.affiliation: Cancer Research and Molecular Biology Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria.
authors.forename: Victor
authors.initials: V
authors.lastname: Akinseye
authors.affiliation: Cancer Research and Molecular Biology Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria.
authors.forename: Oyeronke A
authors.initials: OA
authors.lastname: Odunola
mesh_terms: D000818:Animals; D002235:Carbofuran / Q000633:toxicity*; D008099:Liver / Q000187:drug effects*; D008297:Male; D009153:Mutagens / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D009153:Mutagens; D002235:Carbofuran
keywords: Alanine aminotransferase; Carbamate; Carbofuran; Gamma gluthamyltransferase; Micronucleated polychromatic erythrocytes (mPCEs)