title: Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes.
abstract: Excessive release of stress hormone adrenaline is accompanied by generation of reactive oxygen species which may cause disruption of DNA integrity leading to cancer and age-related disorders. Phenolic-rich plant product dry olive leaf extract (DOLE) is known to modulate effects of various oxidants in human cells. The aim was to evaluate the effect of commercial DOLE against adrenaline induced DNA damage in human leukocytes by using comet assay. Peripheral blood leukocytes from 6 healthy subjects were treated in vitro with three final concentrations of DOLE (0.125, 0.5, and 1mg/mL) for 30 min at 37°C under two different protocols, pretreatment and post-treatment. Protective effect of DOLE was assessed from its ability to attenuate formation of DNA lesions induced by adrenaline. Compared to cells exposed only to adrenaline, DOLE displayed significant reduction (P<0.001) of DNA damage at all three concentrations and under both experimental protocols. Pearson correlation analysis revealed a significant positive association between DOLE concentration and leukocytes DNA damage (P<0.05). Antigenotoxic effect of the extract was more pronounced at smaller concentrations. Post-treatment with 0.125 mg/mL DOLE was the most effective against adrenaline genotoxicity. Results indicate genoprotective and antioxidant properties in dry olive leaf extract, strongly supporting further explorations of its underlying mechanisms of action.
authors.affiliation: Department of Biology and Human Genetics, Institute of Physiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, Belgrade, Serbia. Electronic address: andrea@pharmacy.bg.ac.rs.
authors.forename: Andrea
authors.initials: A
authors.lastname: Cabarkapa
authors.affiliation: Department of Biology and Human Genetics, Institute of Physiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, Belgrade, Serbia. Electronic address: lada@pharmacy.bg.ac.rs.
authors.forename: Lada
authors.initials: L
authors.lastname: Zivković
authors.affiliation: Department of Biology and Human Genetics, Institute of Physiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, Belgrade, Serbia. Electronic address: dijana@pharmacy.bg.ac.rs.
authors.forename: Dijana
authors.initials: D
authors.lastname: Zukovec
authors.affiliation: Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Oslobodjenja Blvd 18, Belgrade, Serbia. Electronic address: ndjelic@sbb.rs.
authors.forename: Ninoslav
authors.initials: N
authors.lastname: Djelić
authors.affiliation: Institute for Research and Development, Galenika a.d., Pasterova 2, 11000 Belgrade, Serbia. Electronic address: vladanbajic@gmail.com.
authors.forename: Vladan
authors.initials: V
authors.lastname: Bajić
authors.affiliation: Institute for Research and Development, Galenika a.d., Pasterova 2, 11000 Belgrade, Serbia. Electronic address: ddekan@sezampro.rs.
authors.forename: Dragana
authors.initials: D
authors.lastname: Dekanski
authors.affiliation: Department of Biology and Human Genetics, Institute of Physiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, Belgrade, Serbia. Electronic address: bilja22@pharmacy.bg.ac.rs.
authors.forename: Biljana
authors.initials: B
authors.lastname: Spremo-Potparević
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000633:toxicity*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007962:Leukocytes / Q000187:drug effects*; D008297:Male; D031658:Olea / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D018515:Plant Leaves; D017382:Reactive Oxygen Species; D055815:Young Adult
chemical_list: D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D004837:Epinephrine
keywords: Adrenaline (CAS 329-63-5); Comet assay; Dry olive leaf extract (DOLE); Oxidative stress