title: Self-illuminating 64Cu-doped CdSe/ZnS nanocrystals for in vivo tumor imaging.
abstract: Construction of self-illuminating semiconducting nanocrystals, also called quantum dots (QDs), has attracted much attention recently due to their potential as highly sensitive optical probes for biological imaging applications. Here we prepared a self-illuminating QD system by doping positron-emitting radionuclide (64)Cu into CdSe/ZnS core/shell QDs via a cation-exchange reaction. The (64)Cu-doped CdSe/ZnS QDs exhibit efficient Cerenkov resonance energy transfer (CRET). The signal of (64)Cu can accurately reflect the biodistribution of the QDs during circulation with no dissociation of (64)Cu from the nanoparticles. We also explored this system for in vivo tumor imaging. This nanoprobe showed high tumor-targeting ability in a U87MG glioblastoma xenograft model (12.7% ID/g at 17 h time point) and feasibility for in vivo luminescence imaging of tumor in the absence of excitation light. The availability of these self-illuminating integrated QDs provides an accurate and convenient tool for in vivo tumor imaging and detection.
authors.affiliation: Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering , National Institutes of Health , Bethesda, Maryland 20892, United States.
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Sun
authors.forename: Xinglu
authors.initials: X
authors.lastname: Huang
authors.forename: Jinxia
authors.initials: J
authors.lastname: Guo
authors.forename: Wenlei
authors.initials: W
authors.lastname: Zhu
authors.forename: Yong
authors.initials: Y
authors.lastname: Ding
authors.forename: Gang
authors.initials: G
authors.lastname: Niu
authors.forename: Andrew
authors.initials: A
authors.lastname: Wang
authors.forename: Dale O
authors.initials: DO
authors.lastname: Kiesewetter
authors.forename: Zhong Lin
authors.initials: ZL
authors.lastname: Wang
authors.forename: Shouheng
authors.initials: S
authors.lastname: Sun
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D019187:Cadmium Compounds / Q000737:chemistry* / Q000493:pharmacokinetics; D003301:Copper Radioisotopes; D004735:Energy Transfer; D049449:Luminescence; D051379:Mice; D009369:Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism; D061848:Optical Imaging; D049268:Positron-Emission Tomography; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry* / Q000493:pharmacokinetics; D013440:Sulfides / Q000737:chemistry* / Q000493:pharmacokinetics; D014018:Tissue Distribution; D023041:Xenograft Model Antitumor Assays; D017967:Zinc Compounds / Q000737:chemistry* / Q000493:pharmacokinetics
chemical_list: D019187:Cadmium Compounds; D003301:Copper Radioisotopes; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; C058667:cadmium selenide; C031238:zinc sulfide