title: Wilms' tumor 1 is involved in tumorigenicity of glioblastoma by regulating cell proliferation and apoptosis.
abstract: The prognosis for patients with glioblastoma is very poor, despite intensive treatment, including surgery and chemoradiotherapy. Wilms' tumor 1 (WT1) is expressed in most glioblastoma samples, and immunotherapy targeting WT1 has proven to be effective in recurrent glioblastoma. However, the functional roles of WT1 in glioblastoma are not clear. To examine the functional roles of WT1 in glioblastoma, glioblastoma cell lines with reduced WT1 expression were generated using short hairpin RNA(shRNA)-expressing lentivirus. Proliferation of WT1-knockdown glioblastoma cells was significantly slower than control cells with high WT1 expression. In addition, apoptosis was increased in WT1-knockdown glioblastoma cells. Furthermore, WT1-knockdown glioblastoma cells, and control glioblastoma cells were intra-cranially injected into immunodeficient mice. In vivo tumor growth of WT1-knockdown glioblastoma cells was significantly reduced compared to control glioblastoma cells. These results show that WT1 is involved in glioblastoma cell proliferation and apoptosis and that this protein has oncogenic roles in glioblastoma.
authors.affiliation: 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan. norikijima@gmail.com.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Kijima
authors.forename: Naoki
authors.initials: N
authors.lastname: Hosen
authors.forename: Naoki
authors.initials: N
authors.lastname: Kagawa
authors.forename: Naoya
authors.initials: N
authors.lastname: Hashimoto
authors.forename: Manabu
authors.initials: M
authors.lastname: Kinoshita
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Oji
authors.forename: Haruo
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Toshiki
authors.initials: T
authors.lastname: Yoshimine
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000502:physiology; D020869:Gene Expression Profiling; D005909:Glioblastoma / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D034741:RNA, Small Interfering / Q000235:genetics; D015996:Survival Rate; D014407:Tumor Cells, Cultured; D025721:WT1 Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D023041:Xenograft Model Antitumor Assays
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D034741:RNA, Small Interfering; C499308:Rag2 protein, mouse; D025721:WT1 Proteins
keywords: Glioblastoma; WT1; apoptosis; tumorigenicity