title: Structural finite element analysis to explain cell mechanics variability.
abstract: The ability to model the mechanical responses of different cell types presents many opportunities to tissue engineering research to further identify changes from physiological conditions to disease. Using a previously validated finite element cell model we aim to show how variation of the material properties of the intracellular components affects cell response after compression and shearing. A parametric study was performed to understand the key mechanical features from different cell types, focussing on specific cytoskeleton components and prestress. Results show that actin cortex does not have a mechanical role in resisting shearing loading conditions. The sensitivity analysis predicted that cell force to compression and shearing is highly affected by changes in cortex thickness, cortex Young's modulus and rigidity of the remaining cytoplasm. Variation of prestress affects mainly the response of cells under shear loads and the model defines a relationship between cell force and prestress depending on the specific loading conditions, which is in good agreement with in vitro experiments. The results are used to make predictions that can relate mechanical properties with cell phenotype to be used as guidelines for individual cytoskeletal structures for future modelling efforts of the structure-function relationships of living cells.
authors.affiliation: INSIGNEO Institute for In Silico Medicine, Department of Mechanical Engineering, University of Sheffield, Mappin Street, Sheffield S1 3JD, United Kingdom.
authors.forename: Sara
authors.initials: S
authors.lastname: Barreto
authors.affiliation: INSIGNEO Institute for In Silico Medicine, Department of Mechanical Engineering, University of Sheffield, Mappin Street, Sheffield S1 3JD, United Kingdom.
authors.forename: Cecile M
authors.initials: CM
authors.lastname: Perrault
authors.affiliation: INSIGNEO Institute for In Silico Medicine, Department of Mechanical Engineering, University of Sheffield, Mappin Street, Sheffield S1 3JD, United Kingdom. Electronic address: d.lacroix@sheffield.ac.uk.
authors.forename: Damien
authors.initials: D
authors.lastname: Lacroix
mesh_terms: D001696:Biomechanical Phenomena; D002448:Cell Adhesion; D002477:Cells / Q000166:cytology*; D020342:Finite Element Analysis; D055595:Mechanical Phenomena
keywords: Actin cortex; Cell model; Cytoskeleton; Finite element analysis; Material properties; Phenotype