title: The effect of a bioactive collagen membrane releasing PDGF or GDF-5 on bone regeneration.
abstract: Regenerative procedures using barrier membrane technology are presently well established in periodontal/endodontic surgery. The objective of this study was to compare the subsequent effects of the released platelet-derived growth factor (PDGF) and growth/differentiation factor 5 (GDF-5) from collagen membranes (CMs) on bone regeneration in vitro and in vivo. In vitro studies were conducted using MC3T3-E1 mouse preosteoblasts cultured with or without factors. Cell viability, cell proliferation, alkaline phosphatase (ALP) activity and bone marker gene expression were then measured. In vivo studies were conducted by placing CMs with low or high dose PDGF or GDF-5 in rat mandibular defects. At 4 weeks after surgery new bone formation was measured using μCT and histological analysis. The results of in vitro studies showed that CM/GDF-5 significantly increased ALP and cell proliferation activities without cytotoxicity in MC3T3-E1 cells when compared to CM/PDGF or CM alone. Gene expression analysis revealed that Runx2 and Osteocalcin were significantly increased in CM/GDF-5 compared to CM/PDGF or control. Quantitative and qualitative μCT and histological analysis for new bone formation revealed that although CM/PDGF significantly enhanced bone regeneration compared to CM alone or control, CM/GDF-5 significantly accelerated bone regeneration to an even greater extent than CM/PDGF. The results also showed that GDF-5 induced new bone formation in a dose-dependent manner. These results suggest that this strategy, using a CM carrying GDF-5, might lead to an improvement in the current clinical treatment of bone defects for periodontal and implant therapy.
authors.affiliation: Department of Prosthodontics, New York University College of Dentistry, New York, NY 10010, USA. Electronic address: sy23@nyu.edu.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Yamano
authors.affiliation: Department of Prosthodontics, New York University College of Dentistry, New York, NY 10010, USA.
authors.forename: Ken
authors.initials: K
authors.lastname: Haku
authors.affiliation: Department of Prosthodontics, New York University College of Dentistry, New York, NY 10010, USA.
authors.forename: Takuto
authors.initials: T
authors.lastname: Yamanaka
authors.affiliation: Department of Prosthodontics, New York University College of Dentistry, New York, NY 10010, USA.
authors.forename: Jisen
authors.initials: J
authors.lastname: Dai
authors.affiliation: Department of Prosthodontics, New York University College of Dentistry, New York, NY 10010, USA.
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Takayama
authors.affiliation: Department of Prosthodontics, New York University College of Dentistry, New York, NY 10010, USA.
authors.forename: Ryutaro
authors.initials: R
authors.lastname: Shohara
authors.affiliation: Department of Prosthodontics, New York University College of Dentistry, New York, NY 10010, USA.
authors.forename: Keita
authors.initials: K
authors.lastname: Tachi
authors.affiliation: Department of Prosthodontics, New York University College of Dentistry, New York, NY 10010, USA.
authors.forename: Mika
authors.initials: M
authors.lastname: Ishioka
authors.affiliation: Department of Prosthodontics, New York University College of Dentistry, New York, NY 10010, USA.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Hanatani
authors.affiliation: Department of Prosthodontics, The University of Tennessee College of Dentistry, Memphis, TN 38103, USA.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Karunagaran
authors.affiliation: Department of Periodontics, University of Pennsylvania School of Dental Medicine, Philadelphia, PA 19104, USA.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Wada
authors.affiliation: Department of Pediatric Dentistry, New York University College of Dentistry, New York, NY 10010, USA.
authors.forename: Amr M
authors.initials: AM
authors.lastname: Moursi
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000378:metabolism*; D015870:Gene Expression; D055428:Growth Differentiation Factor 5 / Q000494:pharmacology*; D051379:Mice; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D051381:Rats; D014157:Transcription Factors
chemical_list: D055428:Growth Differentiation Factor 5; D010982:Platelet-Derived Growth Factor; D014157:Transcription Factors; D015675:Osteocalcin; D003094:Collagen; D000469:Alkaline Phosphatase
keywords: Bone regeneration; Collagen membrane; GDF-5; Growth factor; PDGF