title: Biotechnological procedures to select white rot fungi for the degradation of PAHs.
abstract: White rot fungi are essential in forest ecology and are deeply involved in wood decomposition and the biodegradation of various xenobiotics. The fungal ligninolytic enzymes involved in these processes have recently become the focus of much attention for their possible biotechnological applications. Successful bioremediation requires the selection of species with desirable characteristics. In this study, 150 taxonomically and physiologically diverse white rot fungi, including 55 species, were investigated for their performance in a variety of biotechnological procedures, such as dye decolorization, gallic acid reaction, ligninolytic enzymes, and tolerance to four PAHs, phenanthrene, anthracene, fluoranthene, and pyrene. Among these fungi, six isolates showed the highest (>90%) tolerance to both individual PAH and mixed PAHs. And six isolates oxidized gallic acid with dark brown color and they rapidly decolorized RBBR within ten days. These fungi revealed various profiles when evaluated for their biotechnological performance to compare the capability of degradation of PAHs between two groups selected. As the results demonstrated the six best species selected from gallic acid more greatly degraded four PAHs than the other isolates selected via tolerance test. It provided that gallic acid reaction test can be performed to rank the fungi by their ability to degrade the PAHs. Most of all, Peniophora incarnata KUC8836 and Phlebia brevispora KUC9033 significantly degraded the four PAHs and can be considered prime candidates for the degradation of xenobiotic compounds in environmental settings.
authors.affiliation: Division of Environmental Science & Ecological Engineering, Korea University, Republic of Korea.
authors.forename: Hwanhwi
authors.initials: H
authors.lastname: Lee
authors.affiliation: Division of Environmental Science & Ecological Engineering, Korea University, Republic of Korea.
authors.forename: Yeongseon
authors.initials: Y
authors.lastname: Jang
authors.affiliation: Division of Environmental Science & Ecological Engineering, Korea University, Republic of Korea.
authors.forename: Yong-Seok
authors.initials: YS
authors.lastname: Choi
authors.affiliation: Division of Environmental Science & Ecological Engineering, Korea University, Republic of Korea.
authors.forename: Min-Ji
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Division of Environmental Science & Ecological Engineering, Korea University, Republic of Korea.
authors.forename: Jaejung
authors.initials: J
authors.lastname: Lee
authors.affiliation: Division of Environmental Science & Ecological Engineering, Korea University, Republic of Korea.
authors.forename: Hanbyul
authors.initials: H
authors.lastname: Lee
authors.affiliation: Division of Environmental Science & Ecological Engineering, Korea University, Republic of Korea.
authors.forename: Joo-Hyun
authors.initials: JH
authors.lastname: Hong
authors.affiliation: Division of Environmental Science & Ecological Engineering, Korea University, Republic of Korea.
authors.forename: Young Min
authors.initials: YM
authors.lastname: Lee
authors.affiliation: Division of Environmental Science & Ecological Engineering, Korea University, Republic of Korea.
authors.forename: Gyu-Hyeok
authors.initials: GH
authors.lastname: Kim
authors.affiliation: Division of Environmental Science & Ecological Engineering, Korea University, Republic of Korea. Electronic address: jae-jinkim@korea.ac.kr.
authors.forename: Jae-Jin
authors.initials: JJ
authors.lastname: Kim
mesh_terms: D001487:Basidiomycota / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001709:Biotechnology; D004785:Environmental Pollutants / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism* / Q000494:pharmacology; D013045:Species Specificity
chemical_list: D004785:Environmental Pollutants; D011084:Polycyclic Aromatic Hydrocarbons
keywords: Biodegradation; Polycyclic aromatic hydrocarbons (PAHs); White rot fungi (WRF)