title: HLA-B*57 elite suppressor and chronic progressor HIV-1 isolates replicate vigorously and cause CD4+ T cell depletion in humanized BLT mice.
abstract: Elite controllers or suppressors (ES) are HIV-1-infected patients who maintain undetectable viral loads without antiretroviral therapy. The mechanism of control remains unclear, but the HLA-B*57 allele is overrepresented in cohorts of these patients. However, many HLA-B*57 patients develop progressive disease, and some studies have suggested that infection with defective viruses may be the cause of the lack of high levels of virus replication and disease progression in ES. We therefore performed a comprehensive comparative in vivo and in vitro characterization of viruses isolated from well-defined ES. For this purpose, we first performed full-genome sequence analysis and in vitro fitness assays on replication-competent isolates from HLA-B*57 ES and HLA-B*57 chronic progressors (CPs). Under our experimental conditions, we found that isolates from ES and CPs can replicate in vitro. However, since inherently these assays involve the use of unnaturally in vitro-activated cells, we also investigated the replication competence and pathogenic potential of these HIV isolates in vivo using humanized BLT mice. The results from these analyses demonstrate that virus isolates from ES are fully replication competent in vivo and can induce peripheral and systemic CD4 T cell depletion. These results provide the first direct in vivo evidence that viral fitness does not likely determine clinical outcome in HLA-B*57 patients and that elite suppressors can control replication-competent, fully pathogenic viruses. A better understanding of the immunological bases of viral suppression in ES will serve to inform novel approaches to preventive and therapeutic HIV vaccine design.
  
  
  OBJECTIVE
  Elite suppressors are HIV-1-infected patients who have undetectable levels of viremia despite not being on antiviral drugs. One of the most fundamental questions about this phenomenon involves the mechanism of control. To address this question, we isolated virus from elite suppressors and from HIV-1-infected patients who have the usual progressive disease course. We compared how well the isolates from the two groups of patients replicated in culture and in humanized mice. Our results suggest that elite suppressors are capable of controlling HIV-1 due to the possession of unique host factors rather than infection with defective virus.
authors.affiliation: Department of Medicine, Center for AIDS Research, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Salgado
authors.forename: Michael D
authors.initials: MD
authors.lastname: Swanson
authors.forename: Christopher W
authors.initials: CW
authors.lastname: Pohlmeyer
authors.forename: Robert W
authors.initials: RW
authors.lastname: Buckheit
authors.forename: Jin
authors.initials: J
authors.lastname: Wu
authors.forename: Nancie M
authors.initials: NM
authors.lastname: Archin
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Williams
authors.forename: David M
authors.initials: DM
authors.lastname: Margolis
authors.forename: Robert F
authors.initials: RF
authors.lastname: Siliciano
authors.forename: J Victor
authors.initials: JV
authors.lastname: Garcia
authors.forename: Joel N
authors.initials: JN
authors.lastname: Blankson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000821:virology; D005260:Female; D014644:Genetic Variation; D015658:HIV Infections / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D020276:HIV Long-Term Survivors; D015497:HIV-1 / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D015235:HLA-B Antigens / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008212:Lymphocyte Depletion; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D014779:Virus Replication
chemical_list: D015235:HLA-B Antigens; C103707:HLA-B57 antigen