title: Acid-catalyzed hot-water extraction of lipids from Chlorella vulgaris.
abstract: Acid-catalyzed hot-water treatment for efficient extraction of lipids from a wet microalga, Chlorella vulgaris, was investigated. For an initial fatty acids content of 381.6mg/g cell, the extracted-lipid yield with no heating and no catalyst was 83.2mg/g cell. Under a 1% H2SO4 concentration heated at 120°C for 60min, however, the lipid-extraction yield was 337.4mg/g cell. The fatty acids content, meanwhile, was 935mg fatty acid/g lipid. According to the severity index formula, 337.5mg/g cell of yield under the 1% H2SO4 concentration heated at 150°C for 8min, and 334.2mg/g cell of yield under the 0.5% H2SO4 concentration heated at 150°C for 16min, were obtained. The lipids extracted by acid-catalyzed hot-water treatment were converted to biodiesel. The biodiesel's fatty acid methyl ester (FAME) content after esterification of the microalgal lipids was increased to 79.2% by the addition of excess methanol and sulfuric acid.
authors.affiliation: Department of Clean Fuel, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea. Electronic address: yearn@kier.re.kr.
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Park
authors.affiliation: Department of Clean Fuel, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea.
authors.forename: You-Kwan
authors.initials: YK
authors.lastname: Oh
authors.affiliation: Department of Clean Fuel, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea.
authors.forename: Jin-Suk
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Clean Fuel, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea.
authors.forename: Kyubock
authors.initials: K
authors.lastname: Lee
authors.affiliation: Department of Clean Fuel, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea.
authors.forename: Min-Ji
authors.initials: MJ
authors.lastname: Jeong
authors.affiliation: Department of Clean Fuel, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea.
authors.forename: Sun-A
authors.initials: SA
authors.lastname: Choi
mesh_terms: D056804:Biofuels; D002384:Catalysis / Q000187:drug effects; D048408:Chlorella vulgaris / Q000187:drug effects / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D006358:Hot Temperature; D008055:Lipids / Q000302:isolation & purification*; D011134:Polysaccharides / Q000378:metabolism; D013464:Sulfuric Acids / Q000494:pharmacology*; D014867:Water / Q000494:pharmacology*
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D008055:Lipids; D011134:Polysaccharides; D013464:Sulfuric Acids; D014867:Water; C033158:sulfuric acid
keywords: Acid catalyst; Chlorella vulgaris; Hot-water treatment; Microalgal lipids; Wet extraction