title: Synthetic circuit of inositol phosphorylceramide synthase in Leishmania : a chemical biology approach.
abstract: Building circuits and studying their behavior in cells is a major goal of systems and synthetic biology. Synthetic biology enables the precise control of cellular states for systems studies, the discovery of novel parts, control strategies, and interactions for the design of robust synthetic systems. To the best of our knowledge, there are no literature reports for the synthetic circuit construction for protozoan parasites. This paper describes the construction of genetic circuit for the targeted enzyme inositol phosphorylceramide synthase belonging to the protozoan parasite Leishmania. To explore the dynamic nature of the circuit designed, simulation was done followed by circuit validation by qualitative and quantitative approaches. The genetic circuit designed for inositol phosphorylceramide synthase (Biomodels Database-MODEL1208030000) shows responsiveness, oscillatory and bistable behavior, together with intrinsic robustness.
authors.affiliation: National Centre for Cell Science, NCCS Complex, Ganeshkhind, Pune University Campus, Pune, 411007 India.
authors.forename: Vineetha
authors.initials: V
authors.lastname: Mandlik
authors.affiliation: Department of Computer Science and Technology, Sardar Patel University, Vallabh Vidyanagar, India.
authors.forename: Dixita
authors.initials: D
authors.lastname: Limbachiya
authors.affiliation: National Centre for Cell Science, NCCS Complex, Ganeshkhind, Pune University Campus, Pune, 411007 India.
authors.forename: Sonali
authors.initials: S
authors.lastname: Shinde
authors.affiliation: National Centre for Cell Science, NCCS Complex, Ganeshkhind, Pune University Campus, Pune, 411007 India.
authors.forename: Milsee
authors.initials: M
authors.lastname: Mol
authors.affiliation: National Centre for Cell Science, NCCS Complex, Ganeshkhind, Pune University Campus, Pune, 411007 India.
authors.forename: Shailza
authors.initials: S
authors.lastname: Singh
keywords: Degradation rate; Genetic circuit; IPCS; Leishmania; Logic circuit; Simulation