title: Near-infrared fluorescence probe for the determination of alkaline phosphatase.
abstract: Water-soluble CuInS2 quantum dots (QDs) were directly synthesized in an aqueous solution with mercaptopropionic acid (MPA) as stabilizers, and were functionalized using tryptophan molecules to form tryptophan-functionalized CuInS2 QDs (W-CuInS2 QDs) that had a strong fluorescence emission around 689 nm. The fluorescence of W-CuInS2 QDs could be quenched by Cu(2+) and then the addition of pyrophosphate (PPi) could effectively turn on the quenched fluorescence due to the strong interaction between Cu(2+) and PPi. The alkaline phosphatase (ALP) could catalyze the hydrolysis of PPi that would disassemble the complex of PPi-Cu(2+)-PPi. Therefore, the recovered fluorescence could be quenched again by the addition of ALP. In this paper, we developed a novel near-infrared fluorescence probe for the simple and convenient assay of ALP.
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Siyu
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Pang
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Weidan
authors.initials: W
authors.lastname: Na
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China. Electronic address: suxg@jlu.edu.cn.
authors.forename: Xingguang
authors.initials: X
authors.lastname: Su
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis* / Q000737:chemistry*; D003300:Copper / Q000737:chemistry; D005456:Fluorescent Dyes / Q000032:analysis / Q000138:chemical synthesis; D015336:Molecular Probe Techniques; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence / Q000379:methods*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D014364:Tryptophan / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D003300:Copper; D014364:Tryptophan; D000469:Alkaline Phosphatase
keywords: Alkaline phosphatase; CuInS(2) quantum dots; Near-infrared; Pyrophosphate; Tryptophan