title: Rre37 stimulates accumulation of 2-oxoglutarate and glycogen under nitrogen starvation in Synechocystis sp. PCC 6803.
abstract: Rre37 (sll1330) in a cyanobacterium Synechocystis sp. PCC 6803 acts as a regulatory protein for sugar catabolic genes during nitrogen starvation. Low glycogen accumulation in Δrre37 was due to low expression of glycogen anabolic genes. In addition to low 2-oxoglutarate accumulation, normal upregulated expression of genes encoding glutamate synthases (gltD and gltB) as well as accumulation of metabolites in glycolysis (fructose-6-phosphate, fructose-1,6-bisphosphate, and glyceraldehyde-3-phosphate) and tricarboxylic acid (TCA) cycle (oxaloacetate, fumarate, succinate, and aconitate) were abolished by rre37 knockout. Rre37 regulates 2-oxoglutarate accumulation, glycogen accumulation through expression of glycogen anabolic genes, and TCA cycle metabolites accumulation.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
authors.forename: Ancy
authors.initials: A
authors.lastname: Joseph
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
authors.forename: Shimpei
authors.initials: S
authors.lastname: Aikawa
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Sasaki
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Teramura
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan; Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency, 3-5 Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan.
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Hasunuma
authors.affiliation: RIKEN Biomass Engineering Program, 1-7-22, Suehirocho, Tsurumi, Yokohama 230-0045, Japan; Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University,1-5 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Matsuda
authors.affiliation: PRESTO, Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Osanai
authors.affiliation: RIKEN Center for Sustainable Resource Science, 1-7-22, Suehirocho, Tsurumi, Yokohama 230-0045, Japan.
authors.forename: Masami Yokota
authors.initials: MY
authors.lastname: Hirai
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan; RIKEN Biomass Engineering Program, 1-7-22, Suehirocho, Tsurumi, Yokohama 230-0045, Japan. Electronic address: akondo@kobe-u.ac.jp.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D002952:Citric Acid Cycle; D004268:DNA-Binding Proteins; D005636:Fructosephosphates / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005970:Glutamate Synthase / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism*; D006019:Glycolysis; D007656:Ketoglutaric Acids / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D013217:Starvation; D046939:Synechocystis / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D005636:Fructosephosphates; D007656:Ketoglutaric Acids; C027618:fructose-6-phosphate; D006003:Glycogen; D005970:Glutamate Synthase; D009584:Nitrogen
keywords: 2-OG; 2-Oxoglutarate; 2-oxoglutarate; Cyanobacteria; GOGAT; GS; Glycogen; HPLC; LC/QqQ-MS; Nitrogen starvation; OD; Rre37; TCA; glutamate synthase; glutamine synthetase; high performance liquid chromatography; liquid chromatography/triple quadrupole mass spectrometry; optical density; tricarboxylic acid