title: IL-17A and IL-17F genes variants and susceptibility to childhood asthma in Tunisia.
abstract: OBJECTIVE
  IL-17A and IL-17F are new pro-inflammatory cytokines implicated in neutrophilic inflammation and thus, involved in the pathogenesis of asthma. We investigated the possible association among asthma and IL-17A -197G/A (rs2275913), IL-17F 7488A/G (rs763780) and IL-17F 7383A/G (rs2397084).
  
  
  METHODS
  The study was performed in 171 patients with asthma (mean age 9.5 years, 105 boys, and 66 girls) and 171 healthy individuals matched with patients in age and sex. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to detect genes' polymorphisms.
  
  
  RESULTS
  IL-17A -197G/A and IL-17F 7383A/G were associated with asthma in children (p = 0.008, p = 0.001, respectively). No association was found with IL-17F 7488A/G polymorphism. Haplotype analysis revealed a significant association between GA and AG haplotypes and asthma (p = 0.004, p = 0.02). When patients were stratified according to the atopic status, no significant association was detected with any of the three studied variants.
  
  
  CONCLUSIONS
  Our results suggested that SNPs in IL-17A and IL-17F confer susceptibility to childhood asthma in Tunisia.
authors.affiliation: Faculté de médecine de Tunis, 99/08-40 Unité de Recherche "Homéostasie et disfonctionnement cellulaire" (99/08-40), Université de Tunis El Manar , Tunis , Tunisia and.
authors.forename: Haifa
authors.initials: H
authors.lastname: Maalmi
authors.forename: Anissa
authors.initials: A
authors.lastname: Beraies
authors.forename: Rihab
authors.initials: R
authors.lastname: Charad
authors.forename: Jamel
authors.initials: J
authors.lastname: Ammar
authors.forename: Kamel
authors.initials: K
authors.lastname: Hamzaoui
authors.forename: Agnès
authors.initials: A
authors.lastname: Hamzaoui
mesh_terms: D000293:Adolescent; D017677:Age Distribution; D001249:Asthma / Q000175:diagnosis / Q000453:epidemiology* / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D016001:Confidence Intervals; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D015994:Incidence; D020381:Interleukin-17 / Q000235:genetics*; D008297:Male; D016017:Odds Ratio; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D018570:Risk Assessment; D017678:Sex Distribution; D014416:Tunisia / Q000453:epidemiology
chemical_list: D020381:Interleukin-17