title: A cell-free protein synthesis system from insect cells.
abstract: The newly developed Transdirect in vitro translation system for mRNA templates utilizes an extract from cultured Spodoptera frugiperda 21 (Sf21) insect cells. An expression vector, pTD1, which includes a 5'-untranslated region (UTR) sequence from a baculovirus polyhedrin gene as a translational enhancer, designed to obtain maximum performance from the insect cell-free protein synthesis system. The combination of insect cell extract and the expression vector results in protein productivity of about 50 μg/mL of the translation reaction mixture. This is the highest protein productivity yet recorded among commercialized cell-free protein synthesis systems based on animal extracts.
authors.affiliation: Analytical & Measuring Instruments Division, Clinical & Biotechnology Business Unit, Shimadzu Corporation, Kyoto, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Ezure
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Eiji
authors.initials: E
authors.lastname: Ando
mesh_terms: D000818:Animals; D001483:Base Sequence; D002457:Cell Extracts; D002474:Cell-Free System; D005822:Genetic Vectors / Q000235:genetics; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D061987:Sf9 Cells; D018411:Spodoptera / Q000166:cytology* / Q000821:virology
chemical_list: D002457:Cell Extracts; D012333:RNA, Messenger