title: Rapid isolation of cancer cells using microfluidic deterministic lateral displacement structure.
abstract: This work reports a microfluidic device with deterministic lateral displacement (DLD) arrays allowing rapid and label-free cancer cell separation and enrichment from diluted peripheral whole blood, by exploiting the size-dependent hydrodynamic forces. Experiment data and theoretical simulation are presented to evaluate the isolation efficiency of various types of cancer cells in the microfluidic DLD structure. We also demonstrated the use of both circular and triangular post arrays for cancer cell separation in cell solution and blood samples. The device was able to achieve high cancer cell isolation efficiency and enrichment factor with our optimized design. Therefore, this platform with DLD structure shows great potential on fundamental and clinical studies of circulating tumor cells.
authors.affiliation: Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, People's Republic of China.
authors.forename: Zongbin
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, People's Republic of China ; College of Chemical Engineering, Sichuan University, Chengdu 610065, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Huang
authors.affiliation: Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, People's Republic of China ; Medical Department of Nanchang University Graduate School, Nanchang 330006, People's Republic of China.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Du
authors.affiliation: Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, People's Republic of China.
authors.forename: Weiliang
authors.initials: W
authors.lastname: Shu
authors.affiliation: Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, People's Republic of China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Feng
authors.affiliation: Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, People's Republic of China ; Hong Kong University Shenzhen Hospital, Shenzhen 518053, People's Republic of China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Xu
authors.affiliation: Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen