title: Actin controls the vesicular fraction of dopamine released during extended kiss and run exocytosis.
abstract: The effect of latrunculin A, an inhibitor of actin cross-linking, on exocytosis in PC12 cells was investigated with single cell amperometry. This analysis strongly suggests that the actin cytoskeleton might be involved in regulating exocytosis, especially by mediating the constriction of the pore. In an extended kiss-and-run release mode, actin could actually control the fraction of neurotransmitters released by the vesicle. This scaffold appears to contribute, with the lipid membrane and the protein machinery, to the closing dynamics of the pore, in competition with other forces mediating the opening of the exocytotic channel.
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg , S-41296 Gothenburg, Sweden.
authors.forename: Raphaël
authors.initials: R
authors.lastname: Trouillon
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Ewing
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D000199:Actins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D005089:Exocytosis / Q000187:drug effects / Q000502:physiology*; D008954:Models, Biological; D016716:PC12 Cells; D051381:Rats; D053778:Thiazolidines / Q000494:pharmacology; D022161:Transport Vesicles / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000199:Actins; D019086:Bridged Bicyclo Compounds, Heterocyclic; D053778:Thiazolidines; C037067:latrunculin A; D004298:Dopamine