title: Sunscreen protection against ultraviolet-induced oxidative stress: evaluation of reduced glutathione levels, metalloproteinase secretion, and myeloperoxidase activity.
abstract: Several studies have demonstrated the skin protection by sunscreens considering the aspects skin penetration, photostability, and protection against erythema and sunburn. However, little is known about the effect of topically applied sunscreen formulations on the antioxidant defense, metalloproteinases, and inflammatory processes of skin in response to UVR exposure. Therefore, this study aimed to investigate the use of a cream gel formulation containing the UV filters benzophenone-3, octyl methoxycinnamate, and octyl salicylate to prevent skin damage from a single dose of UVR (2.87 J/cm2). This protective effect was evaluated in vivo by measuring the following biochemical parameters: reduced glutathione levels, secretion of matrix metalloproteinases, and myeloperoxidase activity. The results showed that the sunscreen formulation, despite having sun protection factor (SPF) 15, was not completely effective to protect the skin against GSH depletion, MMP-9 secretion and the inflammatory process induced by UVR. These results demonstrate the importance of analyzing UV-altered biochemical parameters of skin in order to propose new sunscreen formulations that can completely protect skin against UVR-induced damage.
authors.affiliation: Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil.
authors.forename: F M P
authors.initials: FM
authors.lastname: Vilela
authors.affiliation: Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil.
authors.forename: Y M
authors.initials: YM
authors.lastname: Fonseca
authors.affiliation: Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil.
authors.forename: F T M C
authors.initials: FT
authors.lastname: Vicentini
authors.affiliation: Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil.
authors.forename: M J V
authors.initials: MJ
authors.lastname: Fonseca
mesh_terms: D000287:Administration, Topical; D000818:Animals; D002626:Chemistry, Pharmaceutical; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005782:Gels; D005978:Glutathione / Q000378:metabolism*; D008297:Male; D045726:Metalloproteases / Q000378:metabolism*; D051379:Mice; D008812:Mice, Hairless; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D009195:Peroxidase / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000528:radiation effects; D012867:Skin / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D013056:Spectrophotometry, Ultraviolet; D013473:Sunscreening Agents / Q000494:pharmacology*; D014466:Ultraviolet Rays
chemical_list: D005782:Gels; D017382:Reactive Oxygen Species; D013473:Sunscreening Agents; D009195:Peroxidase; D045726:Metalloproteases; D005978:Glutathione