title: Astrocytic and vascular remodeling in the injured adult rat spinal cord after chondroitinase ABC treatment.
abstract: Upregulation of extracellular chondroitin sulfate proteoglycans (CSPG) is a primary cause for the failure of axons to regenerate after spinal cord injury (SCI), and the beneficial effect of their degradation by chondroitinase ABC (ChABC) is widely documented. Little is known, however, about the effect of ChABC treatment on astrogliosis and revascularization, two important factors influencing axon regrowth. This was investigated in the present study. Immediately after a spinal cord hemisection at thoracic level 8-9, we injected ChABC intrathecally at the sacral level, repeated three times until 10 days post-injury. Our results show an effective cleavage of CSPG glycosaminoglycan chains and stimulation of axonal remodeling within the injury site, accompanied by an extended period of astrocyte remodeling (up to 4 weeks). Interestingly, ChABC treatment favored an orientation of astrocytic processes directed toward the injury, in close association with axons at the lesion entry zone, suggesting a correlation between axon and astrocyte remodeling. Further, during the first weeks post-injury, ChABC treatment affected the morphology of laminin-positive blood vessel basement membranes and vessel-independent laminin deposits: hypertrophied blood vessels with detached or duplicated basement membrane were more numerous than in lesioned untreated animals. In contrast, at later time points, laminin expression increased and became more directly associated with newly formed blood vessels, the size of which tended to be closer to that found in intact tissue. Our data reinforce the idea that ChABC injection in combination with other synergistic treatments is a promising therapeutic strategy for SCI repair.
authors.affiliation: 1 Neuroscience Paris Seine/UMR8246/U1130/UMCR18 , IBPS/UPMC Univ Paris 06, Paris, France .
authors.forename: Ulla
authors.initials: U
authors.lastname: Milbreta
authors.forename: Ysander
authors.initials: Y
authors.lastname: von Boxberg
authors.forename: Philippe
authors.initials: P
authors.lastname: Mailly
authors.forename: Fatiha
authors.initials: F
authors.lastname: Nothias
authors.forename: Sylvia
authors.initials: S
authors.lastname: Soares
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects*; D001369:Axons / Q000187:drug effects / Q000473:pathology; D015153:Blotting, Western; D019765:Chondroitin ABC Lyase / Q000494:pharmacology*; D004195:Disease Models, Animal; D005260:Female; D007150:Immunohistochemistry; D009416:Nerve Regeneration / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013119:Spinal Cord Injuries / Q000473:pathology*; D066253:Vascular Remodeling / Q000187:drug effects*
chemical_list: D019765:Chondroitin ABC Lyase