title: Evaluation of endoscopically obtained duodenal biopsy samples from cats and dogs in an adapter-modified Ussing chamber.
abstract: This study was conducted to evaluate an adapter-modified Ussing chamber for assessment of transport physiology in endoscopically obtained duodenal biopsies from healthy cats and dogs, as well as dogs with chronic enteropathies. 17 duodenal biopsies from five cats and 51 duodenal biopsies from 13 dogs were obtained. Samples were transferred into an adapter-modified Ussing chamber and sequentially exposed to various absorbagogues and secretagogues. Overall, 78.6% of duodenal samples obtained from cats responded to at least one compound. In duodenal biopsies obtained from dogs, the rate of overall response ranged from 87.5% (healthy individuals; n = 8), to 63.6% (animals exhibiting clinical signs of gastrointestinal disease and histopathological unremarkable duodenum; n = 15), and 32.1% (animals exhibiting clinical signs of gastrointestinal diseases and moderate to severe histopathological lesions; n = 28). Detailed information regarding the magnitude and duration of the response are provided. The adapter-modified Ussing chamber enables investigation of the absorptive and secretory capacity of endoscopically obtained duodenal biopsies from cats and dogs and has the potential to become a valuable research tool. The response of samples was correlated with histopathological findings.
authors.affiliation: Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843-4474, USA.iruhnke@une.edu.au.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Ruhnke
authors.forename: John V
authors.initials: JV
authors.lastname: DeBiasio
authors.forename: Jan S
authors.initials: JS
authors.lastname: Suchodolski
authors.forename: Shelley
authors.initials: S
authors.lastname: Newman
authors.forename: Mark W
authors.initials: MW
authors.lastname: Musch
authors.forename: Jörg M
authors.initials: JM
authors.lastname: Steiner
mesh_terms: D000818:Animals; D001706:Biopsy / Q000662:veterinary*; D002371:Cat Diseases / Q000503:physiopathology; D002415:Cats / Q000502:physiology*; D004283:Dog Diseases / Q000503:physiopathology; D004285:Dogs / Q000502:physiology*; D004378:Duodenal Diseases / Q000503:physiopathology; D004384:Duodenoscopy / Q000662:veterinary*; D004386:Duodenum / Q000502:physiology* / Q000503:physiopathology
keywords: Ussing chamber; canine; feline; short circuit current; transport physiology