title: Cutaneous leishmaniosis in a horse from northern Portugal.
abstract: The first case of cutaneous leishmaniosis in a horse from the north of Portugal, with a 1.5 cm in diameter ulcerated nodular lesion on the left face, is reported. The skin nodule was surgically excised and assessed by histopathology, including an immunohistochemistry method applied for the first time to equine tissues which clearly demonstrated leishmanial amastigote forms. Two serological determinations with the direct agglutination test performed 13 months apart showed seroconversion specific for Leishmania from a <25 to a 200 antibody titre. Polymerase chain reaction followed by kinetoplast DNA sequencing provided a 116-bp sequence with 98% identity to Leishmania infantum closest sequence deposited in GenBank. No recurrence was observed after complete surgical excision. Leishmaniosis should be included in the differential diagnosis of cutaneous nodular or papular lesions in the equine species in Portugal.
authors.affiliation: Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal; Animal and Veterinary Research Centre (CECAV), UTAD, Vila Real, Portugal.
authors.forename: Adelina
authors.initials: A
authors.lastname: Gama
authors.affiliation: Veterinary Hospital, UTAD, Vila Real, Portugal.
authors.forename: Joana
authors.initials: J
authors.lastname: Elias
authors.affiliation: Veterinary Hospital, UTAD, Vila Real, Portugal.
authors.forename: Ana J
authors.initials: AJ
authors.lastname: Ribeiro
authors.affiliation: Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal.
authors.forename: Nuno
authors.initials: N
authors.lastname: Alegria
authors.affiliation: Koninklijk Instituut voor de Tropen (KIT), Royal Tropical Institute, Department of Parasitology, Amsterdam, The Netherlands.
authors.forename: Henk D F H
authors.initials: HD
authors.lastname: Schallig
authors.affiliation: Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal; Animal and Veterinary Research Centre (CECAV), UTAD, Vila Real, Portugal.
authors.forename: Filipe
authors.initials: F
authors.lastname: Silva
authors.affiliation: Parasite Disease Group, Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, Oporto, Portugal.
authors.forename: Nuno
authors.initials: N
authors.lastname: Santarém
authors.affiliation: Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal; Parasite Disease Group, Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, Oporto, Portugal. Electronic address: lcardoso@utad.pt.
authors.forename: Luís
authors.initials: L
authors.lastname: Cardoso
authors.affiliation: Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal; Animal and Veterinary Research Centre (CECAV), UTAD, Vila Real, Portugal.
authors.forename: Mário
authors.initials: M
authors.lastname: Cotovio
mesh_terms: D000818:Animals; D006734:Horse Diseases / Q000175:diagnosis* / Q000469:parasitology / Q000473:pathology; D006736:Horses; D007891:Leishmania / Q000235:genetics; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D008297:Male; D011174:Portugal
keywords: Cutaneous leishmaniosis; Diagnosis; Horse; Immunohistochemistry; Leishmania; Northern Portugal