title: Cytotoxic steroidal glycosides from Allium flavum.
abstract: Three new spirostane-type glycosides (1-3) were isolated from the whole plant of Allium flavum. Their structures were elucidated mainly by 2D NMR spectroscopic analysis and mass spectrometry as (20S,25R)-2α-hydroxyspirost-5-en-3β-yl O-β-D-xylopyranosyl-(1→3)-[β-D-galactopyranosyl-(1→2)]-β-D-galactopyranosyl-(1→4)-β-D-galactopyranoside (1), (20S,25R)-2α-hydroxyspirost-5-en-3β-yl O-β-D-xylopyranosyl-(1→3)-[β-D-glucopyranosyl-(1→2)]-β-D-galactopyranosyl-(1→4)-β-D-galactopyranoside (2), and (20S,25R)-spirost-5-en-3β-yl O-α-L-rhamnopyranosyl-(1→4)-[β-D-glucopyranosyl-(1→2)]-β-D-glucopyranoside (3). The three saponins were evaluated for cytotoxicity against a human cancer cell line (colorectal SW480).
authors.affiliation: EA 4267, FDE/UFC, Laboratoire de Pharmacognosie, Faculté de Pharmacie, Université de Bourgogne, 21079 Dijon Cedex, France.
authors.forename: Abdelmalek
authors.initials: A
authors.lastname: Rezgui
authors.affiliation: EA 4267, FDE/UFC, Laboratoire de Pharmacognosie, Faculté de Pharmacie, Université de Bourgogne, 21079 Dijon Cedex, France.
authors.forename: Anne-Claire
authors.initials: AC
authors.lastname: Mitaine-Offer
authors.affiliation: Universität Siegen, OC-II, Naturwissenschaftlich-Technische Fakultät, Adolf-Reichwein-Str. 2, D-57076 Siegen, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Paululat
authors.affiliation: Cohiro, UFR Médecine, 7, Bd. Jeanne d'Arc, BP 87900, 21079 Dijon Cedex, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Delemasure
authors.affiliation: Cohiro, UFR Médecine, 7, Bd. Jeanne d'Arc, BP 87900, 21079 Dijon Cedex, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Dutartre
authors.affiliation: EA 4267, FDE/UFC, Laboratoire de Pharmacognosie, Faculté de Pharmacie, Université de Bourgogne, 21079 Dijon Cedex, France. Electronic address: m-a.lacaille-dubois@u-bourgogne.fr.
authors.forename: Marie-Aleth
authors.initials: MA
authors.lastname: Lacaille-Dubois
mesh_terms: D000490:Allium / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D010840:Phytosterols / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D006027:Glycosides; D010840:Phytosterols
keywords: 2D NMR; Allium flavum; Amaryllidaceae; Cytotoxicity; Steroidal saponins