title: sCD30 and sCD40L detection in patients with osteosarcoma, chondrosarcoma and Ewing sarcoma.
abstract: BACKGROUND
  Primary malignant bone tumors are heterogeneous groups of neoplasms, which affect mainly children and adolescents. The most common types are Osteosarcoma, Ewing sarcoma and chondrosarcoma. Elevation of sCD30 and sCD40L has been observed in lymphoma, leukemia and autoimmune disorders.
  
  
  OBJECTIVE
  To evaluate serum concentrations of sCD30 and sCD40L in patients with primary malignant bone tumors.
  
  
  METHODS
  Fifty-four cases (31 Osteosarcomas, 14 Ewing sarcomas, and 9 Chondrosarcomas) and 54 healthy controls enrolled in this study. Cases with the history of prior treatment (surgery, chemotherapy and radiotherapy) were excluded from the study. Serum levels of sCD30 and sCD40L were detected by an enzyme linked immunosorbent assay (ELISA).
  
  
  RESULTS
  Mean serum concentration of sCD30 in Ewing sarcoma was significantly higher than that of the control groups (p=0.007), but mean serum concentrations of sCD30 in osteosarcoma and chondrosarcoma groups were not significantly different, compared to the controls (p=0.41 and p=0.11, respectively). Mean serum concentrations of sCD40L in osteosarcoma, Ewing sarcoma and chondrosarcoma groups were significantly higher than that of the control group (p<0.0001). In addition, the mean serum level of sCD40L in chondrosarcoma patients was higher than that of both Ewing sarcoma and osteosarcoma groups (p<0.001).
  
  
  CONCLUSIONS
  sCD30 and sCD40L increase in primary bone tumors; however the significant of these findings for diagnosis or prognosis of these tumors needs further investigation.
authors.affiliation: Department of Orthopedic Surgery, Bone and Joint Disease Research Center, Chamran Hospital, Shiraz University of Medical Sciences, Shiraz, Iran, saeed_solooki@yahoo.com.
authors.forename: Saeed
authors.initials: S
authors.lastname: Solooki
authors.forename: Arash
authors.initials: A
authors.lastname: Khozaei
authors.forename: Seyedeh Azra
authors.initials: SA
authors.lastname: Shamsdin
authors.forename: Mohammad Jafar
authors.initials: MJ
authors.lastname: Emami
authors.forename: Farnaz
authors.initials: F
authors.lastname: Khademolhosseini
mesh_terms: D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood*; D001859:Bone Neoplasms / Q000175:diagnosis* / Q000276:immunology; D023201:CD40 Ligand / Q000097:blood*; D002648:Child; D002813:Chondrosarcoma / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D006801:Humans; D017730:Ki-1 Antigen / Q000097:blood*; D008297:Male; D012516:Osteosarcoma / Q000175:diagnosis* / Q000276:immunology; D011379:Prognosis; D012512:Sarcoma, Ewing / Q000175:diagnosis* / Q000276:immunology; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D017730:Ki-1 Antigen; D023201:CD40 Ligand