title: A highly sensitive immunoassay using antibody-conjugated spherical mesoporous silica with immobilized enzymes.
abstract: A highly sensitive immunoassay was developed by using antibody-conjugated spherical mesoporous silica with immobilized enzymes. The higher ratio of enzyme/antibody than conventional ELISA improved both the sensitivity and dynamic range. Especially, the use of spherical mesoporous silica could achieve a limit of detection (LOD) with a sensitivity that is 20 times more than that of ELISA using amorphous silica.
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Republic of Korea. jbkim3@korea.ac.kr.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Eum
authors.forename: Sang Youn
authors.initials: SY
authors.lastname: Hwang
authors.forename: Youngjun
authors.initials: Y
authors.lastname: Ju
authors.forename: Jong Min
authors.initials: JM
authors.lastname: Shim
authors.forename: Yunxian
authors.initials: Y
authors.lastname: Piao
authors.forename: Jinwoo
authors.initials: J
authors.lastname: Lee
authors.forename: Hak-Sung
authors.initials: HS
authors.lastname: Kim
authors.forename: Jungbae
authors.initials: J
authors.lastname: Kim
mesh_terms: D004800:Enzymes, Immobilized / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D007118:Immunoassay; D007074:Immunoglobulin G / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D007074:Immunoglobulin G; D012822:Silicon Dioxide; D005949:Glucose Oxidase