title: Astrocyte elevated gene-1 (AEG-1) shRNA sensitizes Huaier polysaccharide (HP)-induced anti-metastatic potency via inactivating downstream P13K/Akt pathway as well as augmenting cell-mediated immune response.
abstract: Astrocyte elevated gene-1 (AEG-1) is an important force in the development and progression of hepatocellular carcinoma (HCC). To extend our study, we examined here the role of AEG-1 in anti-metastatic effects of Huaier polysaccharide (HP) on the human HCC MHCC97-H cell line. AEG-1 shRNA contributed to the anti-proliferation effect of HP on MHCC97-H cells. Furthermore, results of Transwell insert chambers showed that low expression of AEG-1 could effectively facilitate HP to suppress MHCC97-H cell migration and invasion. We achieved this by reducing phosphoinositide 3-kinases (P13K) and phosphorylated Akt (pAkt) expression as well as enhancing natural killer (NK) cell activity. Taken together, our data strongly suggested that AEG-1 shRNA could block the carcinogenesis and progression of MHCC97-H cells and highlight the therapeutic potential of HP in HCC treatment, at least by part, by inhibiting the activation of the PI3K/Akt pathway and enhancing the NK cell-mediated immune response. These findings may provide a new strategy for HCC treatment.
authors.affiliation: Intervention Therapy Center of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing, 100069, China, jiashengzheng@outlook.com.
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Zheng
authors.forename: Cong
authors.initials: C
authors.lastname: Li
authors.forename: Xia
authors.initials: X
authors.lastname: Wu
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Liu
authors.forename: Xicai
authors.initials: X
authors.lastname: Sun
authors.forename: Yingzhuo
authors.initials: Y
authors.lastname: Yang
authors.forename: Meijun
authors.initials: M
authors.lastname: Hao
authors.forename: Shoupeng
authors.initials: S
authors.lastname: Sheng
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.forename: Honghai
authors.initials: H
authors.lastname: Zhang
authors.forename: Jiang
authors.initials: J
authors.lastname: Long
authors.forename: Yi
authors.initials: Y
authors.lastname: Liang
authors.forename: Caixia
authors.initials: C
authors.lastname: Hu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D045744:Cell Line, Tumor; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D008113:Liver Neoplasms / Q000276:immunology / Q000473:pathology; D008565:Membrane Proteins; D009362:Neoplasm Metastasis / Q000517:prevention & control; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology; D000081082:Phosphoinositide-3 Kinase Inhibitors; D011134:Polysaccharides / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors* / Q000502:physiology; D034741:RNA, Small Interfering / Q000235:genetics*; D016601:RNA-Binding Proteins; D015398:Signal Transduction / Q000502:physiology*; D055454:Trametes / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D015815:Cell Adhesion Molecules; D008565:Membrane Proteins; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D011134:Polysaccharides; D051057:Proto-Oncogene Proteins c-akt; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; C483039:MTDH protein, human