title: Circadian rhythms in Neurospora crassa on a polydimethylsiloxane microfluidic device for real-time gas perturbations.
abstract: Racetubes, a conventional system employing hollow glass tubes, are typically used for monitoring circadian rhythms from the model filamentous fungus, Neurospora crassa. However, a major technical limitation in using a conventional system is that racetubes are not amenable for real-time gas perturbations. In this work, we demonstrate a simple microfluidic device combined with real-time gas perturbations for monitoring circadian rhythms in Neurospora crassa using bioluminescence assays. The developed platform is a useful toolbox for investigating molecular responses under various gas conditions for Neurospora and can also be applied to other microorganisms.
authors.affiliation: Microsystems and BioMEMS Laboratory, School of Electronics and Computing Systems, University of Cincinnati, Cincinnati, Ohio 45221, USA.
authors.forename: Kang Kug
authors.initials: KK
authors.lastname: Lee
authors.affiliation: Microsystems and BioMEMS Laboratory, School of Electronics and Computing Systems, University of Cincinnati, Cincinnati, Ohio 45221, USA.
authors.forename: Chong H
authors.initials: CH
authors.lastname: Ahn
authors.affiliation: Computational and Molecular Biology Laboratory, Department of Molecular and Cellular Physiology, University of Cincinnati, Cincinnati, Ohio 45267, USA.
authors.forename: Christian I
authors.initials: CI
authors.lastname: Hong