title: Higher circulating levels of chemokine CCL20 in patients with multiple sclerosis: evaluation of the influences of chemokine gene polymorphism, gender, treatment and disease pattern.
abstract: Chemokines play an important role in the autoimmune diseases. The aim of this study was to investigate the levels of CCL20 and a polymorphism [-786C > T (rs6749704)] in the chemokine gene in patients with multiple sclerosis (MS). The blood samples were collected from 135 MS patients and 135 healthy subjects as a control group. The patients have relapsing-remitting (RRMS; n = 65), primary progressive (PPMS; n = 47), secondary progressive (SPMS; n = 35) or progressive relapsing (PRMS; n = 14) patterns. The serum levels of CCL20 were measured by ELISA. The DNA was analyzed for CCL20 polymorphism using PCR-RLFP. The mean serum levels of CCL20 in the MS group were significantly higher than in the healthy group (P < 0.001). In patients with a SPMS pattern, the frequency of CT genotype at rs6749704 (24.3 %) was significantly lower as compared to patients with other patterns (42.8 %; P < 0.04). No significant differences were observed between subjects with different genotypes in rs6749704 regarding the CCL20 levels. The mean serum levels of CCL20 in both newly diagnosed and previously diagnosed patients was significantly higher than in the healthy group (P < 0.05 and 0.001, respectively). The mean serum levels of CCL20 in patients with RRMS, SPMS and PPMS patterns were significantly higher than in the healthy group (P < 0.004, P < 0.04, and 0.05, respectively). The levels of CCL20 in untreated patients and in patients who received interferon-β, methylprednisolone or the combination of interferon-β plus methylprednisolone were higher as compared to the control group (P < 0.05, P < 0.03, P < 0.005, and P < 0.05, respectively). These results showed higher levels of CCL20 in patients that represent that the chemokine may play an important role in the pathogenesis of MS. The rs6749704 polymorphism was an associated SPMS pattern. The levels of CCL20 were not influenced by gender, disease pattern and treatment.
authors.affiliation: Neurology Research Center, Department of Neurology, Kerman University of Medical Sciences, Kerman, Iran, Jafarzadeh@kmu.ac.ir.
authors.forename: A
authors.initials: A
authors.lastname: Jafarzadeh
authors.forename: S
authors.initials: S
authors.lastname: Bagherzadeh
authors.forename: H A
authors.initials: HA
authors.lastname: Ebrahimi
authors.forename: H
authors.initials: H
authors.lastname: Hajghani
authors.forename: M R
authors.initials: MR
authors.lastname: Bazrafshani
authors.forename: A
authors.initials: A
authors.lastname: Khosravimashizi
authors.forename: M
authors.initials: M
authors.lastname: Nemati
authors.forename: F
authors.initials: F
authors.lastname: Gadari
authors.forename: A
authors.initials: A
authors.lastname: Sabahi
authors.forename: F
authors.initials: F
authors.lastname: Iranmanesh
authors.forename: M M
authors.initials: MM
authors.lastname: Mohammadi
authors.forename: H
authors.initials: H
authors.lastname: Daneshvar
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D054418:Chemokine CCL20 / Q000097:blood / Q000235:genetics*; D005260:Female; D006801:Humans; D016899:Interferon-beta / Q000627:therapeutic use; D008297:Male; D008775:Methylprednisolone / Q000627:therapeutic use; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000175:diagnosis / Q000188:drug therapy / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012737:Sex Factors
chemical_list: C108355:CCL20 protein, human; D054418:Chemokine CCL20; D016899:Interferon-beta; D008775:Methylprednisolone