title: Prevalence of day-care centre children (France) with faecal CTX-M-producing Escherichia coli comprising O25b:H4 and O16:H5 ST131 strains.
abstract: OBJECTIVE
  Determining the prevalence of children in day-care centres (DCCs) carrying faecal extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae and molecularly characterizing those belonging to the Escherichia coli species.
  
  
  METHODS
  Stools were collected from children's diapers (January-April 2012) in randomly chosen DCCs and plated onto ChromID ESBL. Colonies growing on this medium were identified by the Vitek 2 system and tested for antibiotic susceptibility and for ESBL production by the double-disc synergy test. ESBL genotypes were determined as well as phylogenetic groups, ERIC-2 (enterobacterial repetitive intergenic consensus) PCR profiles and sequence types (STs) for the E. coli isolates. Serotypes, virotypes, fimH alleles, ESBL-carrying plasmids and PFGE patterns were determined for the ST131 E. coli isolates.
  
  
  RESULTS
  Among 419 children from 25 participating DCCs, 1 was colonized by CTX-M-15-producing Klebsiella pneumoniae and 27 (6.4%) by E. coli, which all produced CTX-M enzymes [CTX-M-15 (37%), CTX-M-1 (26%), CTX-M-14 (22%), CTX-M-27 (11%) and CTX-M-22 (4%)]. The 27 E. coli isolates, 55.5% belonging to group B2, displayed 20 ERIC-2 PCR profiles and 16 STs. The ST131 E. coli isolates were dominant (44%), displayed serotypes O25b:H4 and O16:H5, fimH alleles 30 and 41 and virotypes A and C. According to the PFGE patterns, one strain of E. coli ST131 producing a CTX-M-15 enzyme carried by an IncF F2:A1:B- plasmid had spread within one DCC.
  
  
  CONCLUSIONS
  This study shows a notable prevalence (6.4%) of DCC children with faecal CTX-M-producing E. coli isolates comprising a high proportion of E. coli ST131 isolates, suggesting that these children might be a reservoir of this clone.
authors.affiliation: Service de Biologie, Centre Hospitalier d'Antibes-Juan les Pins, Antibes, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Blanc
authors.forename: Véronique
authors.initials: V
authors.lastname: Leflon-Guibout
authors.forename: Jorge
authors.initials: J
authors.lastname: Blanco
authors.forename: Marisa
authors.initials: M
authors.lastname: Haenni
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Madec
authors.forename: Gwenaële
authors.initials: G
authors.lastname: Rafignon
authors.forename: Pascale
authors.initials: P
authors.lastname: Bruno
authors.forename: Azucena
authors.initials: A
authors.lastname: Mora
authors.forename: Cecilia
authors.initials: C
authors.lastname: Lopez
authors.forename: Ghizlane
authors.initials: G
authors.lastname: Dahbi
authors.forename: Brigitte
authors.initials: B
authors.lastname: Dunais
authors.forename: Magali
authors.initials: M
authors.lastname: Anastay
authors.forename: Catherine
authors.initials: C
authors.lastname: Branger
authors.forename: Richard
authors.initials: R
authors.lastname: Moreau
authors.forename: Christian
authors.initials: C
authors.lastname: Pradier
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Nicolas-Chanoine
mesh_terms: D002656:Child Day Care Centers; D002675:Child, Preschool; D016000:Cluster Analysis; D003430:Cross-Sectional Studies; D004926:Escherichia coli / Q000145:classification* / Q000201:enzymology / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D005260:Female; D005602:France / Q000453:epidemiology; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D058889:Molecular Typing; D010802:Phylogeny; D015995:Prevalence; D012703:Serotyping; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D001618:beta-Lactamases
keywords: E. coli; fimH-based subclones; plasmids; virotypes