title: Disruption of biofilm formation by the human pathogen Acinetobacter baumannii using engineered quorum-quenching lactonases.
abstract: Acinetobacter baumannii is a major human pathogen associated with multidrug-resistant nosocomial infections; its virulence is attributed to quorum-sensing-mediated biofilm formation, and disruption of biofilm formation is an attractive antivirulence strategy. Here, we report the first successful demonstration of biofilm disruption in a clinical isolate of A. baumannii S1, using a quorum-quenching lactonase obtained by directed evolution; this engineered lactonase significantly reduced the biomass of A. baumannii-associated biofilms, demonstrating the utility of this antivirulence strategy.
authors.affiliation: Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Jeng Yeong
authors.initials: JY
authors.lastname: Chow
authors.forename: Yuanyong
authors.initials: Y
authors.lastname: Yang
authors.forename: Song Buck
authors.initials: SB
authors.lastname: Tay
authors.forename: Kim Lee
authors.initials: KL
authors.lastname: Chua
authors.forename: Wen Shan
authors.initials: WS
authors.lastname: Yew
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism / Q000494:pharmacology*; D019020:Directed Molecular Evolution / Q000379:methods; D008826:Microbial Sensitivity Tests; D018613:Microscopy, Confocal; D053038:Quorum Sensing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D002265:Carboxylic Ester Hydrolases