title: DNA microarray-based gene expression profiling of estrogenic chemicals.
abstract: We summarize updated information about DNA microarray-based gene expression profiling by focusing on its application to estrogenic chemicals. First, estrogenic chemicals, including natural/industrial estrogens and phytoestrogens, and the methods for detection and evaluation of estrogenic chemicals were overviewed along with a comprehensive list of estrogenic chemicals of natural or industrial origin. Second, gene expression profiling of chemicals using a focused microarray containing estrogen-responsive genes is summarized. Third, silent estrogens, a new type of estrogenic chemicals characterized by their estrogenic gene expression profiles without growth stimulative or inhibitory effects, have been identified so far exclusively by DNA microarray assay. Lastly, the prospect of a microarray assay is discussed, including issues such as commercialization, future directions of applications and quality control methods.
authors.affiliation: Signaling Molecules Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan, kiyama.r@aist.go.jp.
authors.forename: Ryoiti
authors.initials: R
authors.lastname: Kiyama
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D045744:Cell Line, Tumor; D052244:Endocrine Disruptors / Q000494:pharmacology*; D004967:Estrogens / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D046228:Microarray Analysis; D020411:Oligonucleotide Array Sequence Analysis; D064209:Phytochemicals / Q000494:pharmacology*; D048789:Phytoestrogens / Q000494:pharmacology*; D011786:Quality Control; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D052244:Endocrine Disruptors; D004967:Estrogens; D064209:Phytochemicals; D048789:Phytoestrogens; D011960:Receptors, Estrogen