title: Rapid identification of linezolid resistance in Enterococcus spp. based on high-resolution melting analysis.
abstract: The polymorphism G2576T in the 23S rRNA gene is the mutation most frequently associated with linezolid resistance in Enterococcus spp. isolates. We designed a high-resolution melting procedure to detect G2576T which succeeded in identifying linezolid resistant isolates and classified them according to the number of 23S rRNA copies mutated.
authors.affiliation: Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; CIBER Enfermedades Respiratorias-CIBERES (CD06/06/0058), Spain.
authors.forename: María
authors.initials: M
authors.lastname: Alonso
authors.affiliation: Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; CIBER Enfermedades Respiratorias-CIBERES (CD06/06/0058), Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Marín
authors.affiliation: Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; CIBER Enfermedades Respiratorias-CIBERES (CD06/06/0058), Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Iglesias
authors.affiliation: Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; CIBER Enfermedades Respiratorias-CIBERES (CD06/06/0058), Spain.
authors.forename: Emilia
authors.initials: E
authors.lastname: Cercenado
authors.affiliation: Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; CIBER Enfermedades Respiratorias-CIBERES (CD06/06/0058), Spain; Facultad de Medicina, Universidad Complutense de Madrid (UCM), Spain.
authors.forename: Emilio
authors.initials: E
authors.lastname: Bouza
authors.affiliation: Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; CIBER Enfermedades Respiratorias-CIBERES (CD06/06/0058), Spain; CEI Campus Moncloa, UCM-UPM, Madrid, Spain. Electronic address: dgviedma2@gmail.com.
authors.forename: Darío
authors.initials: D
authors.lastname: García de Viedma
mesh_terms: D000081:Acetamides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016983:Enterococcus / Q000235:genetics*; D000069349:Linezolid; D023303:Oxazolidinones / Q000494:pharmacology*; D011110:Polymorphism, Genetic / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics
chemical_list: D000081:Acetamides; D000900:Anti-Bacterial Agents; D023303:Oxazolidinones; D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S; D000069349:Linezolid
keywords: 23S rRNA copies; Enterococcus; G2576T; HRM