title: Tcea3 regulates the vascular differentiation potential of mouse embryonic stem cells.
abstract: Tcea3 is present in high concentrations in mouse embryonic stem cells (mESCs) and functions to activate Lefly1, a negative regulator of Nodal signaling. The Nodal pathway has numerous biological activities, including mesoderm induction and patterning in early embryogenesis. Here, we demonstrate that the suppression of Tcea3 in mESCs shifts the cells from pluripotency into enhanced mesoderm development. Vascular endothelial growth factor A (VEGFA) and VEGFC, major transcription factors that regulate vasculogenesis, are activated in Tcea3 knocked down (Tcea3 KD) mESCs. Moreover, differentiating Tcea3 KD mESCs have perturbed gene expression profiles with suppressed ectoderm and activated mesoderm lineage markers. Most early differentiating Tcea3 KD cells expressed Brachyury-T, a mesoderm marker, whereas control cells did not express the gene. Finally, development of chimeric embryos that included Tcea3 KD mESCs was perturbed.
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seoul, Korea.
authors.forename: Young
authors.initials: Y
authors.lastname: Cha
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seoul, Korea.
authors.forename: Sun-Hee
authors.initials: SH
authors.lastname: Heo
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seoul, Korea.
authors.forename: Hee-Jin
authors.initials: HJ
authors.lastname: Ahn
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seoul, Korea.
authors.forename: Seong Kyu
authors.initials: SK
authors.lastname: Yang
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seoul, Korea.
authors.forename: Ji-Hwan
authors.initials: JH
authors.lastname: Song
authors.affiliation: College of Pharmacy, Ajou University, Suwon, Korea.
authors.forename: Wonhee
authors.initials: W
authors.lastname: Suh
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seoul, Korea.
authors.forename: Kyung-Soon
authors.initials: KS
authors.lastname: Park
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D020869:Gene Expression Profiling; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D035602:Transcriptional Elongation Factors / Q000235:genetics / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: D035602:Transcriptional Elongation Factors; D042461:Vascular Endothelial Growth Factor A; C018648:transcription factor S-II