title: H2A.B facilitates transcription elongation at methylated CpG loci.
abstract: H2A.B is a unique histone H2A variant that only exists in mammals. Here we found that H2A.B is ubiquitously expressed in major organs. Genome-wide analysis of H2A.B in mouse ES cells shows that H2A.B is associated with methylated DNA in gene body regions. Moreover, H2A.B-enriched gene loci are actively transcribed. One typical example is that H2A.B is enriched in a set of differentially methylated regions at imprinted loci and facilitates transcription elongation. These results suggest that H2A.B positively regulates transcription elongation by overcoming DNA methylation in the transcribed region. It provides a novel mechanism by which transcription is regulated at DNA hypermethylated regions.
authors.affiliation: Division of Molecular Medicine and Genetics, Department of Internal Medicine.
authors.forename: Yibin
authors.initials: Y
authors.lastname: Chen
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.forename: Richard C
authors.initials: RC
authors.lastname: McEachin
authors.forename: James D
authors.initials: JD
authors.lastname: Cavalcoli
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Yu
mesh_terms: D000818:Animals; D018899:CpG Islands; D019175:DNA Methylation / Q000235:genetics*; D005786:Gene Expression Regulation; D014644:Genetic Variation; D016678:Genome; D006657:Histones / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D006657:Histones