title: Effect of biomass adaptation to the degradation of anionic surfactants in laundry wastewater using EGSB reactors.
abstract: Two expanded granular sludge bed reactors were operated. RAB (adapted biomass) was operated in two stages: Stage I, with standard LAS (13.2 mg L(-1)); and Stage II, in which the standard LAS was replaced by diluted laundry wastewater according to the LAS concentration (11.2 mg L(-1)). RNAB (not adapted biomass) had a single stage, using direct wastewater (11.5 mg L(-1)). Thus, the strategy of biomass adaptation did not lead to an increase of surfactant removal in wastewater (RAB-Stage II: 77%; RNAB-Stage I: 78%). By means of denaturing gradient gel electrophoresis, an 80% similarity was verified in the phases with laundry wastewater (sludge bed) despite the different reactor starting strategies. By pyrosequencing, many reads were related to genera of degraders of aromatic compounds and sulfate reducers (Syntrophorhabdus and Desulfobulbus). The insignificant difference in LAS removal between the two strategies was most likely due to the great microbial richness of the inoculum.
authors.affiliation: Laboratório de Processos Biológicos (LPB), Departamento de Hidráulica e Saneamento, Escola de Engenharia de São Carlos (EESC), Universidade de São Paulo (USP), Engenharia Ambiental - Bloco 4-F, Av. João Dagnone, 1100 - Santa Angelina, 13563-120 São Carlos, SP, Brazil. Electronic address: tiago.palladino@gmail.com.
authors.forename: T P
authors.initials: TP
authors.lastname: Delforno
authors.affiliation: Laboratório de Processos Biológicos (LPB), Departamento de Hidráulica e Saneamento, Escola de Engenharia de São Carlos (EESC), Universidade de São Paulo (USP), Engenharia Ambiental - Bloco 4-F, Av. João Dagnone, 1100 - Santa Angelina, 13563-120 São Carlos, SP, Brazil. Electronic address: alanamou@hotmail.com.
authors.forename: A G L
authors.initials: AG
authors.lastname: Moura
authors.affiliation: Laboratório de Processos Biológicos (LPB), Departamento de Hidráulica e Saneamento, Escola de Engenharia de São Carlos (EESC), Universidade de São Paulo (USP), Engenharia Ambiental - Bloco 4-F, Av. João Dagnone, 1100 - Santa Angelina, 13563-120 São Carlos, SP, Brazil. Electronic address: dagokada@gmail.com.
authors.forename: D Y
authors.initials: DY
authors.lastname: Okada
authors.affiliation: Laboratório de Processos Biológicos (LPB), Departamento de Hidráulica e Saneamento, Escola de Engenharia de São Carlos (EESC), Universidade de São Paulo (USP), Engenharia Ambiental - Bloco 4-F, Av. João Dagnone, 1100 - Santa Angelina, 13563-120 São Carlos, SP, Brazil. Electronic address: varesche@sc.usp.br.
authors.forename: M B A
authors.initials: MB
authors.lastname: Varesche
mesh_terms: D017738:Alkanesulfonic Acids / Q000737:chemistry; D000838:Anions; D001673:Biodegradation, Environmental; D018533:Biomass; D019149:Bioreactors; D016000:Cluster Analysis; D058645:Denaturing Gradient Gel Electrophoresis; D010316:Particle Size; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology; D013501:Surface-Active Agents / Q000302:isolation & purification*; D062065:Wastewater / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
chemical_list: D017738:Alkanesulfonic Acids; D000838:Anions; D012722:Sewage; D013501:Surface-Active Agents; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Anaerobic reactor; Expanded granular sludge bed (EGSB); Linear alkylbenzene sulfonate (LAS); Pyrosequencing