title: Expression profile of IL-35 mRNA in gingiva of chronic periodontitis and aggressive periodontitis patients: a semiquantitative RT-PCR study.
abstract: BACKGROUND
  Proinflammatory and anti-inflammatory cytokines play a key role in the pathogenesis of periodontal diseases. Secretion of bioactive IL-35 has been described by T regulatory cells (T(regs)) and is required for their maximal suppressive activity. T(regs) are involved in the modulation of local immune response in chronic periodontitis patients.
  
  
  OBJECTIVE
  Hence, the present study was aimed to investigate the expression of IL-35 mRNA in chronic periodontitis and aggressive periodontitis patients.
  
  
  METHODS
  The present study was carried out in 60 subjects, which included 20 chronic periodontitis patients, 20 aggressive periodontitis patients, and 20 periodontally healthy controls. IL-35 mRNA expression in gingival tissue samples of all subjects was semiquantitatively analyzed using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR).
  
  
  RESULTS
  The present study demonstrated the expression of IL-35 mRNA in gingival tissues of all the three groups. IL-35 mRNA expression was highest in chronic periodontitis subjects (6.87 ± 2.32) as compared to the aggressive periodontitis group (4.71 ± 1.43) and least seen in healthy patients (3.03 ± 1.91).
  
  
  CONCLUSIONS
  The increased expression of IL-35 in chronic and aggressive periodontitis suggests its possible role in pathogenesis of periodontitis. Future studies done on large samples with intervention will strengthen our result.
authors.affiliation: Department of Periodontics, PMNM Dental College, Bagalkot, Karnataka 587101, India.
authors.forename: Nagaraj B
authors.initials: NB
authors.lastname: Kalburgi
authors.affiliation: Department of Periodontics, PMNM Dental College, Bagalkot, Karnataka 587101, India.
authors.forename: Akshay
authors.initials: A
authors.lastname: Muley
authors.affiliation: Department of Periodontics, Rajarajeswari Dental College and Hospital, Ramohalli Cross, Mysore Road, Bangalore, Karnataka 560060, India.
authors.forename: B M
authors.initials: BM
authors.lastname: Shivaprasad
authors.affiliation: Department of Periodontics, PMNM Dental College, Bagalkot, Karnataka 587101, India.
authors.forename: Arati C
authors.initials: AC
authors.lastname: Koregol
mesh_terms: D000328:Adult; D010520:Aggressive Periodontitis / Q000378:metabolism*; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D005881:Gingiva / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D007378:Interleukins; D012333:RNA, Messenger; C532417:interleukin-35, human