title: Transient folate deprivation facilitates the generation of mouse-induced pluripotent stem cells.
abstract: Somatic cells can be reprogrammed into iPS (induced pluripotent stem) cells through the ectopic expression of defined transcription factors. However, the inefficiency and amount of time needed limited the potential application of iPS cells. We report an efficient method to generate iPS cells from MEF (mouse embryonic fibroblasts) through folate-depriviatoin, which was used to change the methylation of MEF. Without folate for 3 days, the induction efficiency is enhanced fivefold. Karyotype analysis showed that transient folate-depriving treatment did not negatively affect properties of iPS cells; characterised iPS cells show normal karyotypes. Thus, a new method has been found that can improve the induction efficiency, but not increase the chance of chromosomal mutation.
authors.affiliation: Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China; Department of Neurology, Cangzhou Central Hospital, Cangzhou, 061001, China.
authors.forename: Qiuyue
authors.initials: Q
authors.lastname: Yan
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.forename: Wentao
authors.initials: W
authors.lastname: Hu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Suming
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D005492:Folic Acid / Q000008:administration & dosage* / Q000378:metabolism*; D005494:Folic Acid Deficiency / Q000378:metabolism; D057026:Induced Pluripotent Stem Cells / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D013997:Time Factors
chemical_list: D005492:Folic Acid
keywords: folate; induced pluripotent stem (iPS) cells; murine; reprogramming