title: Adoptive transfer of genetically engineered WT1-specific cytotoxic T lymphocytes does not induce renal injury.
abstract: Because WT1 is expressed in leukemia cells, the development of cancer immunotherapy targeting WT1 has been an attractive translational research topic. However, concern of this therapy still remains, since WT1 is abundantly expressed in renal glomerular podocytes. In the present study, we clearly showed that WT1-specific cytotoxic T lymphocytes (CTLs) certainly exerted cytotoxicity against podocytes in vitro; however, they did not damage podocytes in vivo. This might be due to the anatomical localization of podocytes, being structurally separated from circulating CTLs in glomerular capillaries by an exceptionally thick basement membrane.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Asai
authors.affiliation: Department of Hematology, Clinical Immunology, and Infectious Diseases, Ehime University Graduate School of Medicine, Toon, Ehime, Japan. yunarief@m.ehime-u.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fujiwara
authors.forename: Sohei
authors.initials: S
authors.lastname: Kitazawa
authors.forename: Naoto
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Toshiki
authors.initials: T
authors.lastname: Ochi
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Miyazaki
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Ochi
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Akatsuka
authors.forename: Sachiko
authors.initials: S
authors.lastname: Okamoto
authors.forename: Junichi
authors.initials: J
authors.lastname: Mineno
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Kuzushima
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ikeda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shiku
authors.forename: Masaki
authors.initials: M
authors.lastname: Yasukawa
mesh_terms: D019264:Adoptive Transfer / Q000379:methods*; D000818:Animals; D002460:Cell Line; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D005434:Flow Cytometry; D005818:Genetic Engineering; D059871:HLA-A24 Antigen / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000276:immunology* / Q000378:metabolism; D007678:Kidney Glomerulus / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D050199:Podocytes / Q000276:immunology / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism / Q000637:transplantation*; D025721:WT1 Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: C438506:HLA-A*24:02 antigen; D059871:HLA-A24 Antigen; D025721:WT1 Proteins