title: [Alkaloids from gorgonian-derived Streptomyces sp. SCSGAA0009 and their antibacterial and antilarval activities].
abstract: OBJECTIVE
  To search for structurally novel and biologically active compounds from the secondary metabolites of gorgonian-derived actinomyces.
  
  
  METHODS
  Strains of actinomyces with antimicrobial activities were screened by biological methods. Then, those active strains were cultured under different conditions to obtain crude extracts. Subsequently, the chemical diversities of the extracts were investigated by reverse phase high-performance liquid chromatography (RP-HPLC) and thin layer chromatography (TLC), while the bioactivities of the extracts were evaluated by antifouling and antibacterial assays. A target strain Streptomyces sp. SCSGAA0009 was selected to further study by large-scale fermentation on modified ISP2 medium. The compounds were isolated by column chromatography over silica gel, Sephadex LH-20, and semipreparative HPLC, and their structures were determinated by spectroscopic analysis.
  
  
  RESULTS
  The target strain was identified as actinomyces, Streptomyces sp. SCSGAA0009. A new alkaloid N-(2-(1H-indol-3-yl)ethyl) propionamide (1) and a known compound phenazine-1-carboxylic acid (2) were purified from the culture broth. Compound 2 showed moderate antibacterial activity against Escherichia coli and Pseudoaltermonas piscida, and exhibited strong antilarval settlement activity towards Bugula neritina larva.
  
  
  CONCLUSIONS
  Bioactive and new compounds can be achieved from the secondary metabolites of gorgonian-derived microorganisms from South China Sea.
authors.affiliation: Key Laboratory of Marine Bio-resources Sustainable Utilization/Guangdong Key Laboratory of Marine Materia Medica/RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China. nongxuhua4883@163.com
authors.forename: Xuhua
authors.initials: X
authors.lastname: Nong
authors.affiliation: Key Laboratory of Marine Bio-resources Sustainable Utilization/Guangdong Key Laboratory of Marine Materia Medica/RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Ninth People's Hospital of Shenzhen, Shenzhen 518116, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Key Laboratory of Marine Bio-resources Sustainable Utilization/Guangdong Key Laboratory of Marine Materia Medica/RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
authors.forename: Shuhua
authors.initials: S
authors.lastname: Qi
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D037421:Anthozoa / Q000382:microbiology*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D002013:Bryozoa / Q000187:drug effects / Q000254:growth & development; D004926:Escherichia coli / Q000187:drug effects; D007814:Larva / Q000187:drug effects* / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010619:Phenazines / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D031161:Pseudoalteromonas / Q000187:drug effects; D012623:Seawater / Q000382:microbiology*; D064210:Secondary Metabolism; D013302:Streptomyces / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000470:Alkaloids; D000900:Anti-Bacterial Agents; D010619:Phenazines; C037165:1-phenazinecarboxylic acid