title: [Influence of the gelatin-alginate matrixes with calcium lactate in plasma coagulation system after implantation in soft tissues].
abstract: BACKGROUND
  Bioresorbable porous substrates from copolymers have their application in tissue engineering to culture tissues in vitro. The advantage of polymers is the production of thermoplastic elements and their ability to biodegrade in a living body. Gelatin, collagen, alginates are part of dressings used for topical administration of the drug. Research was undertaken to achieve a porous gelatin-alginate matrix which could be used in therapy as among others, a carrier for a drug.
  
  
  OBJECTIVE
  Evaluation of the impact of modified gelatin-alginate matrix on activation of plasma coagulation in vivo.
  
  
  METHODS
  Gelatin-alginate matrix cross-linked with calcium ions was implanted in the muscle tissue of a rat. The control group constituted animals not implanted with material, but they passed the operating procedure. Blood samples of plasma coagulation test and control group were collected after 1, 2, 3, 5, 7, 10, 14 days of the procedure.
  
  
  RESULTS
  Prolongation of APTT and shortening of PT and TT with the unchanged values of fibrinogen and the count of platelet cells was observed till the 5th day on the basis of the obtained results. Prolongation of APTT with the unchanged values of the remaining parameters of the coagulation system was observed after 7, 10 and 14 days with unchanged values of PT and TT coagulation.
  
  
  CONCLUSIONS
  The matrix gelatin-alginate with calcium ions in the biological environment undergoes biodegradation in soft tissues. This process in the initial period influences the activation of the coagulation within the intrinsic and extrinsic system. From the 5th to 14th day the activation of coagulation was observed only in the intrinsic system.
authors.affiliation: Zakład Chirurgii Eksperymentalnej i Badania Biomateriałów, Uniwersytet Medyczny im. Piastów Slaskich we Wrocławiu, Wrocław, Polska. maria.szymonowicz@umed.wroc.pl
authors.forename: Maria
authors.initials: M
authors.lastname: Szymonowicz
authors.affiliation: Zakład Chirurgii Eksperymentalnej i Badania Biomateriałów, Uniwersytet Medyczny im. Piastów Slaskich we Wrocławiu, Wrocław, Polska.
authors.forename: Bogusława
authors.initials: B
authors.lastname: Zywicka
authors.affiliation: Zakład Chirurgii Eksperymentalnej i Badania Biomateriałów, Uniwersytet Medyczny im. Piastów Slaskich we Wrocławiu, Wrocław, Polska.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Rybak
authors.affiliation: Zakład Chirurgii Eksperymentalnej i Badania Biomateriałów, Uniwersytet Medyczny im. Piastów Slaskich we Wrocławiu, Wrocław, Polska.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Rusak
authors.affiliation: Katedra i Zakład Technologii Postaci Leku, Uniwersytet Medyczny im. Piastów Slaskich we Wrocławiu, Wrocław, Polska.
authors.forename: Dorota
authors.initials: D
authors.lastname: Haznar
authors.affiliation: Katedra i Zakład Technologii Postaci Leku, Uniwersytet Medyczny im. Piastów Slaskich we Wrocławiu, Wrocław, Polska.
authors.forename: Janusz
authors.initials: J
authors.lastname: Pluta
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects*; D017610:Calcium Compounds / Q000494:pharmacology*; D003432:Cross-Linking Reagents; D004337:Drug Carriers / Q000494:pharmacology*; D005781:Gelatin Sponge, Absorbable / Q000494:pharmacology*; D007773:Lactates / Q000494:pharmacology*; D008297:Male; D018482:Muscle, Skeletal / Q000601:surgery; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001672:Biocompatible Materials; D017610:Calcium Compounds; D003432:Cross-Linking Reagents; D004337:Drug Carriers; D007773:Lactates; C110051:calcium lactate