title: Intra-specific regulatory variation in Drosophila pseudoobscura.
abstract: It is generally accepted that gene regulation serves an important role in determining the phenotype. To shed light on the evolutionary forces operating on gene regulation, previous studies mainly focused on the expression differences between species and their inter-specific hybrids. Here, we use RNA-Seq to study the intra-specific distribution of cis- and trans-regulatory variation in Drosophila pseudoobscura. Consistent with previous results, we find almost twice as many genes (26%) with significant trans-effects than genes with significant cis-effects (18%). While this result supports the previous suggestion of a larger mutational target of trans-effects, we also show that trans-effects may be subjected to purifying selection. Our results underline the importance of intra-specific analyses for the understanding of the evolution of gene expression.
authors.affiliation: Institut für Populationsgenetik, Vetmeduni Vienna, Vienna, Austria ; Vienna Graduate School of Population Genetics, Vienna, Austria.
authors.forename: Anton
authors.initials: A
authors.lastname: Suvorov
authors.affiliation: Institut für Populationsgenetik, Vetmeduni Vienna, Vienna, Austria.
authors.forename: Viola
authors.initials: V
authors.lastname: Nolte
authors.affiliation: Institut für Populationsgenetik, Vetmeduni Vienna, Vienna, Austria.
authors.forename: Ram Vinay
authors.initials: RV
authors.lastname: Pandey
authors.affiliation: Institut für Populationsgenetik, Vetmeduni Vienna, Vienna, Austria.
authors.forename: Susanne U
authors.initials: SU
authors.lastname: Franssen
authors.affiliation: Institut für Populationsgenetik, Vetmeduni Vienna, Vienna, Austria ; Department of Applied Statistics, Johannes Kepler Universität Linz, Linz, Austria.
authors.forename: Andreas
authors.initials: A
authors.lastname: Futschik
authors.affiliation: Institut für Populationsgenetik, Vetmeduni Vienna, Vienna, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Schlötterer
mesh_terms: D000483:Alleles; D000818:Animals; D005075:Biological Evolution; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D040582:Inheritance Patterns; D008297:Male; D058977:Molecular Sequence Annotation; D010641:Phenotype; D013045:Species Specificity
chemical_list: D029721:Drosophila Proteins