title: Interaction between nanoparticles generated by zinc chloride treatment and oxidative responses in rat liver.
abstract: The aim of the present study was to investigate the interaction of zinc chloride (3 mg/kg, intraperitoneally [ip]) in rat liver in terms of the biosynthesis of nanoparticles. Zinc treatment increased zinc content in rat liver. Analysis of fluorescence revealed the presence of red fluorescence in the liver following zinc treatment. Interestingly, the co-exposure to zinc (3 mg/kg, ip) and selenium (0.20 mg/L, per os [by mouth]) led to a higher intensity of red fluorescence compared to zinc-treated rats. In addition, X-ray diffraction measurements carried out on liver fractions of zinc-treated rats point to the biosynthesis of zinc sulfide and/or selenide nanocomplexes at nearly 51.60 nm in size. Moreover, co-exposure led to nanocomplexes of about 72.60 nm in size. The interaction of zinc with other mineral elements (S, Se) generates several nanocomplexes, such as ZnS and/or ZnSe. The nanocomplex ZnX could interact directly with enzyme activity or indirectly by the disruption of mineral elements' bioavailability in cells. Subacute zinc or selenium treatment decreased malondialdehyde levels, indicating a drop in lipid peroxidation. In addition, antioxidant enzyme assays showed that treatment with zinc or co-treatment with zinc and selenium increased the activities of glutathione peroxidase, catalase, and superoxide dismutase. Consequently, zinc complexation with sulfur and/or selenium at nanoscale level could enhance antioxidative responses, which is correlated to the ratio of number of ZnX nanoparticles (X=sulfur or X=selenium) to malondialdehyde level in rat liver.
authors.affiliation: Laboratory of Integrative Physiology, Faculty of Sciences of Bizerte, Carthage University, Tunisia.
authors.forename: Inès
authors.initials: I
authors.lastname: Azzouz
authors.affiliation: Laboratory of Integrative Physiology, Faculty of Sciences of Bizerte, Carthage University, Tunisia.
authors.forename: Hamdi
authors.initials: H
authors.lastname: Trabelsi
authors.affiliation: Laboratory of Integrative Physiology, Faculty of Sciences of Bizerte, Carthage University, Tunisia.
authors.forename: Amel
authors.initials: A
authors.lastname: Hanini
authors.affiliation: Laboratory of Integrative Physiology, Faculty of Sciences of Bizerte, Carthage University, Tunisia.
authors.forename: Soumaya
authors.initials: S
authors.lastname: Ferchichi
authors.affiliation: Laboratory of Integrative Physiology, Faculty of Sciences of Bizerte, Carthage University, Tunisia.
authors.forename: Olfa
authors.initials: O
authors.lastname: Tebourbi
authors.affiliation: Laboratory of Integrative Physiology, Faculty of Sciences of Bizerte, Carthage University, Tunisia.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Sakly
authors.affiliation: Laboratory of Integrative Physiology, Faculty of Sciences of Bizerte, Carthage University, Tunisia.
authors.forename: Hafedh
authors.initials: H
authors.lastname: Abdelmelek
mesh_terms: D000818:Animals; D002712:Chlorides / Q000493:pharmacokinetics*; D008099:Liver / Q000737:chemistry* / Q000378:metabolism*; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012643:Selenium / Q000737:chemistry*; D018038:Sodium Selenite / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D015032:Zinc / Q000378:metabolism*; D017967:Zinc Compounds / Q000493:pharmacokinetics*
chemical_list: D002712:Chlorides; D017382:Reactive Oxygen Species; D017967:Zinc Compounds; C016837:zinc chloride; D012643:Selenium; D018038:Sodium Selenite; D015032:Zinc
keywords: X-ray diffraction; antioxidative responses; fluorescence microscopy; liver; nanocomplexes biosynthesis