title: Association between LMP2/LMP7 genetic variability and the metastasis risk of ovarian cancer in Chinese women in Beijing.
abstract: LMP2/LMP7 gene (LMP, low molecular mass protein) perform a critical role in the foreign antigen processing via the major histocompatibility complex-I (MHC-I) complex CD8(+) cytotoxic T lymphocytes (CTL) pathway. This study was designed to investigate whether the sequence variants in LMP2/LMP7 gene would increase the risk of ovarian cancer in the Chinese population. Total of 235 patients with ovarian cancer and 338 normal controls were recruited. Two polymorphisms of LMP2-60 (Arg→His) and LMP7-145 (Gln→Lys) were identified by PCR-RFLP (RFLP, restriction fragment length polymorphism) method. The association of LMP2/LMP7 gene variations with ovarian cancer was assessed by logistic regression analysis. The results revealed that LMP7-145 Gln/Lys and Lys/Lys alleles were associated with the risk of ovarian cancer (P=0.002, OR=2.47; P<0.001, OR=3.23). Meanwhile, the relationship between the LMP7-145 polymorphism and the lymph node metastasis and tumor distant metastasis were also found. No statistical correlation between any of the LMP2-60 polymorphic genotypes and the ovarian cancer clinicopathological characteristics were observed (P>0.05). These results suggested that LMP7 genetic variant could increase the susceptibility to ovarian cancer development; especially increase the risk of lymph node and tumor distant metastasis.
authors.affiliation: Department of Physiology and Pathophysiology, Basic Medical College, Capital Medical University, Beijing 100069, China.
authors.forename: Li
authors.initials: L
authors.lastname: Song
authors.affiliation: Department of Oncology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Nina
authors.initials: N
authors.lastname: Ma
authors.affiliation: Department of Oncology, People's Hospital of Beijing Daxing District, Capital Medical University, Beijing 102600, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.affiliation: Department of Oncology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Han
authors.initials: H
authors.lastname: Yan
authors.affiliation: Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, TX 75390, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Yan
authors.affiliation: Department of Oncology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Zhenyan
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: Department of Oncology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China. Electronic address: oncologychina@163.com.
authors.forename: Bangwei
authors.initials: B
authors.lastname: Cao
mesh_terms: D000328:Adult; D002277:Carcinoma / Q000235:genetics / Q000276:immunology*; D002681:China; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000235:genetics; D010051:Ovarian Neoplasms / Q000235:genetics / Q000276:immunology*; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism*; D012306:Risk
chemical_list: C071313:LMP-2 protein; D003546:Cysteine Endopeptidases; C077436:LMP7 protein; D046988:Proteasome Endopeptidase Complex