title: Comprehensive analysis of protein interactions between JAZ proteins and bHLH transcription factors that negatively regulate jasmonate signaling.
abstract: Jasmonates have crucial roles in plant responses to biotic and abiotic stresses. Given the importance of transcriptional regulation in jasmonate-mediated stress responses, transcription factors are key regulators of jasmonate signaling. The transcription factors JASMONATE-ASSOCIATED MYC2-LIKE 1 (JAM1), JAM2, and JAM3 are negative regulators of jasmonate signaling, although the mechanisms that control the activities of these transcription factors remain unclear. To understand the regulatory mechanisms of JAM proteins, we used a yeast two-hybrid assay to screen for protein interaction partners of JAM1 and found that JAM1 interacted with JAZ proteins.
authors.affiliation: RIKEN Plant Science Center; Tsurumi-ku, Yokohama Kanagawa, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Sasaki-Sekimoto
authors.affiliation: Graduate School of Bioscience and Biotechnology; Tokyo Institute of Technology; Nagatsuta-cho Midori-ku Yokohama Kanagawa, Japan.
authors.forename: Hikaru
authors.initials: H
authors.lastname: Saito
authors.affiliation: Center for Biological Resources and Informatics; Tokyo Institute of Technology; Nagatsuta-cho Midori-ku Yokohama Kanagawa, Japan; Earth-Life Science Institute; Tokyo Institute of Technology; Ookayama Meguro-ku; Tokyo, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Masuda
authors.affiliation: RIKEN Center for Sustainable Resource Science; Suehiro-cho, Tsurumi-ku, Yokohama Kanagawa, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Shirasu
authors.affiliation: Center for Biological Resources and Informatics; Tokyo Institute of Technology; Nagatsuta-cho Midori-ku Yokohama Kanagawa, Japan; Earth-Life Science Institute; Tokyo Institute of Technology; Ookayama Meguro-ku; Tokyo, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohta
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism*; D054883:Oxylipins / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D003517:Cyclopentanes; D054883:Oxylipins; D012097:Repressor Proteins; C011006:jasmonic acid
keywords: Arabidopsis thaliana; JAZ; bHLH transcription factor; jasmonate signaling; protein-protein interaction