title: Expression analysis of two cancer-testis genes, FBXO39 and TDRD4, in breast cancer tissues and cell lines.
abstract: Breast cancer accounts for one third of new cancer cases among women. The need for biomarkers for early detection is the stimulus to researchers to evaluate altered expression of genes in tumours. Cancer-testis (CT) genes are a group with limited expression in normal tissues except testis but up-regulation in a wide variety of cancers. We here evaluated expression of two CT genes named FBXO39 and TDRD4 in 32 invasive ductal carcinoma samples, 10 fibroadenomas and 6 normal breast tissue samples, in addition to two breast cancer cell lines, MCF-7 and MDA-MB-231, by the means of quantitative real time RT-PCR. FBXO39 showed significant up-regulation in invasive ductal carcinoma samples in comparison with normal samples. It also was expressed in both cell lines and after RHOXF1 gene knock down it was down-regulated in MCF-7 but up-regulated in the MDA-MB-231 cell line. TDRD4 was not expressed in the MCF-7 cell line and any of the tissue samples except testis. However, it was expressed in MDA-MB-231 and was up-regulated after RHOXF1 gene knock down. Our results show that FBXO39 but not TDRD4 can be used for cancer detection and if proved to be immunogenic, might be a putative candidate for breast cancer immunotherapy.
authors.affiliation: Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran E-mail : ghafourifard@razi.tims.ac.ir.
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Seifi-Alan
authors.forename: Roshanak
authors.initials: R
authors.lastname: Shamsi
authors.forename: Soudeh
authors.initials: S
authors.lastname: Ghafouri-Fard
authors.forename: Reza
authors.initials: R
authors.lastname: Mirfakhraie
authors.forename: Davood
authors.initials: D
authors.lastname: Zare-Abdollahi
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Movafagh
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Modarressi
authors.forename: Golnesa
authors.initials: G
authors.lastname: Kazemi
authors.forename: Lobat
authors.initials: L
authors.lastname: Geranpayeh
authors.forename: Mitra
authors.initials: M
authors.lastname: Najafi-Ashtiani
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics* / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D044783:F-Box Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D018226:Fibroadenoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012261:Ribonucleoproteins / Q000235:genetics* / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; D044783:F-Box Proteins; C559726:FBXO39 protein, human; D012333:RNA, Messenger; C560514:RNF17 protein, human; D012261:Ribonucleoproteins