title: Next-generation sequencing-based transcriptome analysis of L-lysine-producing Corynebacterium glutamicum ATCC 21300 strain.
abstract: In the present study, 151 genes showed a significant change in their expression levels in Corynebacterium glutamicum ATCC 21300 compared with those of C. glutamicum ATCC 13032. Of these 151 genes, 56 genes (2%) were up-regulated and 95 genes (3%) were down-regulated. RNA sequencing analysis also revealed that 11 genes, involved in the L-lysine biosynthetic pathway of C. glutamicum, were up- or down-regulated compared with those of C. glutamicum ATCC 13032. Of the 151 genes, 10 genes were identified to have mutations including SNP (9 genes) and InDel (1 gene). This information will be useful for genome breeding of C. glutamicum to develop an industrial amino acid-producing strain with minimal mutation.
authors.affiliation: Department of Biomedical Chemistry, Konkuk University, Chung-Ju, 380-701, Republic of Korea.
authors.forename: Hong-Il
authors.initials: HI
authors.lastname: Kim
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Nam
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Cho
authors.forename: Chang-Soo
authors.initials: CS
authors.lastname: Lee
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Park
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D059014:High-Throughput Nucleotide Sequencing; D008239:Lysine / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D059467:Transcriptome
chemical_list: D001426:Bacterial Proteins; D008239:Lysine