title: Plasmid construction by SLIC or sequence and ligation-independent cloning.
abstract: Sequence and ligation-independent cloning (Nat Methods 4:251-256, 2007) is a powerful tool for the construction of multi-fragment complex plasmids in a simple and efficient manner. Plasmids consisting of 6-7 DNA fragments can be assembled in a single day, with additional 2 days for screening and extraction. SLIC requires PCR products with overlapping regions of 30-40 bp at the 5' and 3' ends, T4 DNA polymerase, and an optional RecA protein for construction.
authors.affiliation: Department of Biochemistry, University of Otago, Dunedin, New Zealand.
authors.forename: Ryan E
authors.initials: RE
authors.lastname: Hill
authors.forename: Julian J
authors.initials: JJ
authors.lastname: Eaton-Rye
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers / Q000235:genetics / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase