title: Salvianolic acid B attenuates spinal cord ischemia-reperfusion-induced neuronal injury and oxidative stress by activating the extracellular signal-regulated kinase pathway in rats.
abstract: BACKGROUND
  Salvianolic acid B (SalB), the main bioactive compound isolated from the traditional Chinese medicinal herb broad Radix Salviae Miltiorrhizae exerts a spectrum of pharmacologic activities. We investigated the effects of SalB treatment in a rat model of spinal cord ischemia and reperfusion (I/R) injury and the underlying mechanism.
  
  
  METHODS
  SalB was administered at 1, 10, or 50 mg/kg after spinal cord ischemia. The potential protective effects on spinal cord injury were determined by spinal cord edema, infarct volume, and motor function assessment of the hind limbs.
  
  
  RESULTS
  SalB treatment significantly decreased spinal cord edema and infarct volume and preserved motor function of the hind limbs in a dose-dependent manner. SalB administration ameliorated the generation of oxidative products and preserved antioxidant defense activities in the injured spinal cord at both 4 and 24 h after I/R injury. Moreover, SalB prolonged the I/R injury-induced activation of extracellular signal-regulated kinase (ERK), and blocking ERK activation with PD98059 partially prevented the neuroprotective effects of SalB.
  
  
  CONCLUSIONS
  These findings demonstrate the neuroprotective effects of SalB in a spinal cord I/R injury model and suggest that SalB-induced neuroprotection was mediated by ERK activation.
authors.affiliation: Department of Orthopaedic Oncology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Fu
authors.affiliation: Department of Orthopaedic Oncology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Hong-bin
authors.initials: HB
authors.lastname: Fan
authors.affiliation: Department of Orthopaedic Oncology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China. Electronic address: fmmuguozheng@163.com.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Department of Orthopaedic Oncology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China. Electronic address: fmmuwangzhen@163.com.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Orthopaedic Oncology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Xiang-dong
authors.initials: XD
authors.lastname: Li
authors.affiliation: Department of Orthopaedic Oncology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Orthopaedic Oncology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Guo-xian
authors.initials: GX
authors.lastname: Pei
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001572:Benzofurans / Q000494:pharmacology / Q000627:therapeutic use*; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D006439:Hemodynamics; D008124:Locomotion / Q000187:drug effects; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000201:enzymology / Q000517:prevention & control*; D013119:Spinal Cord Injuries / Q000201:enzymology / Q000517:prevention & control*; D053630:Depsides
chemical_list: D000975:Antioxidants; D001572:Benzofurans; D004365:Drugs, Chinese Herbal; C076944:salvianolic acid B; D053630:Depsides
keywords: Ischemia-reperfusion injury; Neuroprotection; Oxidative stress; Salvianolic acid B