title: Phytosphigosine-1-phosphate increases sensitivity of EGF-dependent cell proliferation.
abstract: The dermis is composed of dermal fibroblasts and various synthesized extracellular matrices. Proliferation of these cells is important to skin structure homeostasis. Therefore, human dermal fibroblasts (HDFs) growth factors have been previously evaluated. In the present study, we examined whether phytosphingosine-1-phosphate (PhS1P) regulates gene expression, particularly cell cycle-related genes. In addition, we investigated whether there was a synergistic effect of proliferation induced by PhS1P and epidermal growth factor (EGF) through PhS1P-regulated genes. A microarray approach was utilized to identify gene expression changes in PhS1P-treated HDFs and data were analyzed using gene ontology (GO). In addition, proliferative synergistic effects were measured using an MTT assay. The results showed that PhS1P regulates various genes, particularly cell cycle-related genes. Microarray data, followed by GO, indicated that PhS1P affected the biological processes involved in the cell cycle (cyclins A2, B1 and B2). Furthermore, these genes synergistically affected EGF-dependent proliferation. The results obtained in this study demonstrated that PhS1P altered gene expression profiles, inducing EGF-dependent cell proliferation. Therefore, PhS1p acts as a synergistic effector for EGF.
authors.affiliation: Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Hwa Jun
authors.initials: HJ
authors.lastname: Cha
authors.affiliation: Coreana Cosmetics Co., Ltd., Seonggeo-eup, Seobuk-gu, Cheonan-si, Chungcheongnam-do 330-833, Republic of Korea.
authors.forename: Jeong Pyo
authors.initials: JP
authors.lastname: Lee
authors.affiliation: Coreana Cosmetics Co., Ltd., Seonggeo-eup, Seobuk-gu, Cheonan-si, Chungcheongnam-do 330-833, Republic of Korea.
authors.forename: Kwang Sik
authors.initials: KS
authors.lastname: Lee
authors.affiliation: Coreana Cosmetics Co., Ltd., Seonggeo-eup, Seobuk-gu, Cheonan-si, Chungcheongnam-do 330-833, Republic of Korea.
authors.forename: Kun Kook
authors.initials: KK
authors.lastname: Lee
authors.affiliation: Phytos Co. Ltd., No. 609, Industry-Academic Collaboration Foundation, Yeongtong-gu, Gyeonggi-do 443-760, Republic of Korea.
authors.forename: Myoung Jun
authors.initials: MJ
authors.lastname: Choi
authors.affiliation: Industrial Engineering Chemistry, Chungbuk National University, Heungdeok-gu, Cheongju, Chungcheongbuk-do 361-763, Republic of Korea.
authors.forename: Dong Kyu
authors.initials: DK
authors.lastname: Lee
authors.affiliation: Damy Chemical Co., Ltd., No. 308, Byuk-San Digital Valley II, Keumchun-gu, Seoul 153-803, Republic of Korea.
authors.forename: Kwang Nyeon
authors.initials: KN
authors.lastname: Kim
authors.affiliation: Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Sungkwan
authors.initials: S
authors.lastname: An
mesh_terms: D018797:Cell Cycle Proteins / Q000096:biosynthesis; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D020405:Dermis / Q000166:cytology / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000235:genetics / Q000378:metabolism*; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005346:Fibroblast Growth Factors; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D012867:Skin / Q000166:cytology / Q000187:drug effects; D013110:Sphingosine / Q000008:administration & dosage / Q000031:analogs & derivatives*; D046888:Tissue Array Analysis
chemical_list: D018797:Cell Cycle Proteins; C117422:phytosphingosine-1-phosphate; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D013110:Sphingosine