title: miR-145 functions as tumor suppressor and targets two oncogenes, ANGPT2 and NEDD9, in renal cell carcinoma.
abstract: OBJECTIVE
  Abnormal expression of miRNAs is closely related to a variety of human cancers. The purpose of this study is to identify new tumor suppressor miRNA and elucidate its physiological function and mechanism in renal cell carcinoma (RCC).
  
  
  METHODS
  The expression of miR-145 in 45 RCC and adjacent normal tissues was performed by quantitative RT-PCR. Cell proliferation, migration, invasion, apoptosis and cycle assays were carried out for functional analysis after miR-145 transfection. Two target genes of miR-145 were identified by luciferase reporter assay. The altered expression of 84 epithelial to mesenchymal transition (EMT)-related genes after miR-145 transfection was detected by RT(2) Profiler EMT PCR array.
  
  
  RESULTS
  The expression of miR-145 was downregulated in RCC compared to their normal adjacent tissues. Restoring miR-145 expression in RCC cell lines dramatically suppressed cell proliferation, migration and invasion, and induced cell apoptosis and G2-phase arrest. We further validated those miR-145 targets two oncogenes, ANGPT2 and NEDD9 in RCC. In addition, miR-145 was found to regulate numerous genes involved in the EMT.
  
  
  CONCLUSIONS
  These findings demonstrate that miR-145 functions as tumor suppressor in RCC, suggesting that miR-145 may be a potential therapeutic target for RCC.
authors.affiliation: Department of Clinical Laboratory, Peking University Shenzhen Hospital, Shenzhen, 518036, China.
authors.forename: Ruijing
authors.initials: R
authors.lastname: Lu
authors.forename: Ziliang
authors.initials: Z
authors.lastname: Ji
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Li
authors.forename: Qingna
authors.initials: Q
authors.lastname: Zhai
authors.forename: Chunjuan
authors.initials: C
authors.lastname: Zhao
authors.forename: Zhimao
authors.initials: Z
authors.lastname: Jiang
authors.forename: Shiqiang
authors.initials: S
authors.lastname: Zhang
authors.forename: Liping
authors.initials: L
authors.lastname: Nie
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Yu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000187:drug effects / Q000235:genetics*; D000328:Adult; D000368:Aged; D042702:Angiopoietin-2 / Q000235:genetics*; D016588:Anticarcinogenic Agents / Q000378:metabolism* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001483:Base Sequence; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D008875:Middle Aged; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D009857:Oncogenes; D010750:Phosphoproteins / Q000187:drug effects / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C586513:ANGPT2 protein, human; D048868:Adaptor Proteins, Signal Transducing; D042702:Angiopoietin-2; D016588:Anticarcinogenic Agents; C541010:MIRN145 microRNA, human; D035683:MicroRNAs; C099942:NEDD9 protein, human; D010750:Phosphoproteins