title: Recombinant adenovirus-p53 (Gendicine) sensitizes a pancreatic carcinoma cell line to radiation.
abstract: OBJECTIVE
  In this study, we examine the effects of recombinant adenovirus-p53 (rAd-p53) on the pancreatic carcinoma cell line SW1990. Specifically, we determine if expression of rAd-p53 sensitizes these cells to radiation.
  
  
  METHODS
  Following transfection of SW1990 cells with rAd-p53, we measured expression of P53, P21 and Bax by immunocytochemistry. Both transfected and control cell lines were irradiated with a range of doses, and the survival fractions (SF) were calculated. Dose survival curves were constructed and modeled for comparison.
  
  
  RESULTS
  Transfection of SW1990 cells with rAd-p53 resulted in increased expression of P53, P21 and Bax in a time-dependent manner. At 96 h after transfection, 89.92% of cells expressed P53, 56.8% expressed P21, and 76.50% expressed Bax. The SF following radiation was lower in the rAd-p53 transfected cells compared to the control cells, suggesting that rAd-p53 sensitizes SW1990 cells to radiation (D0 for the experimental and control groups was 2.199 and 2.462, respectively).
  
  
  CONCLUSIONS
  Use of the adenoviral vector is an effective means of transfecting SW1990 cells with wild-type P53, and this sensitizes the cell line to irradiation. This work suggests that combining rAd-p53 with radiation therapy in pancreatic cancer may be therapeutically beneficial.
authors.affiliation: Department of Radiation Oncology, Teaching Hospital of Fujian Medical University, Fujian Provincial Cancer Hospital, Fuzhou 350014, China;
authors.forename: Jinluan
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Radiation Oncology, Teaching Hospital of Fujian Medical University, Fujian Provincial Cancer Hospital, Fuzhou 350014, China;
authors.forename: Jianji
authors.initials: J
authors.lastname: Pan
authors.affiliation: Department of Radiation Oncology, Peking University People's Hospital, Beijing 100044, China;
authors.forename: Xianggao
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Radiation Oncology, Teaching Hospital of Fujian Medical University, Fujian Provincial Cancer Hospital, Fuzhou 350014, China;
authors.forename: Ying
authors.initials: Y
authors.lastname: Su
authors.affiliation: Department of Radiation Oncology, Teaching Hospital of Fujian Medical University, Fujian Provincial Cancer Hospital, Fuzhou 350014, China;
authors.forename: Lingling
authors.initials: L
authors.lastname: Bao
authors.affiliation: Department of Radiation Oncology, Teaching Hospital of Fujian Medical University, Fujian Provincial Cancer Hospital, Fuzhou 350014, China;
authors.forename: Sufang
authors.initials: S
authors.lastname: Qiu
authors.affiliation: Department of Radiation Oncology, Teaching Hospital of Fujian Medical University, Fujian Provincial Cancer Hospital, Fuzhou 350014, China;
authors.forename: Changyan
authors.initials: C
authors.lastname: Zou
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiation Oncology, Peking University Cancer Hospital & Institute, Beijing 100142, China;
authors.forename: Yong
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Radiation Oncology, Teaching Hospital of Fujian Medical University, Fujian Provincial Cancer Hospital, Fuzhou 350014, China;
authors.forename: Junxin
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Radiation Oncology, National University Cancer Institute, Singapore 119228, Singapore.
authors.forename: Ivan W K
authors.initials: IW
authors.lastname: Tham
keywords: Pancreatic carcinoma; radiosensitization; recombinant adenovirus-p53 (rAd-p53); transfection