title: Photocleavage control of nucleated DNA nanosystems--the influence of surface strand sterics.
abstract: We use sterically inaccessible 'seed' strands, released from a surface into solution by photocleavage to initiate a nucleated DNA polymerization reaction. We demonstrate control of the quantity of 'seed' release and that hairpin steric protection of the 'seed' leads to less 'leaky' surfaces. This polymerization is a model system for surface-photocleavage initiation of sub-stoichiometric reaction cascades; these cascades should find use as a component of labs-on-chips capable of bioanalytical and DNA-computing tasks.
authors.affiliation: Chemistry Department, St. John's University, 8000 Utopia Parkway Queens, NY 11439, USA. lukemanp@stjohns.edu.
authors.forename: Morcos
authors.initials: M
authors.lastname: Hanna
authors.forename: Moorsalin
authors.initials: M
authors.lastname: Munshi
authors.forename: Nancy A
authors.initials: NA
authors.lastname: Kedzierski
authors.forename: Paul N
authors.initials: PN
authors.lastname: Chung
authors.forename: Terry
authors.initials: T
authors.lastname: Huang
authors.forename: Allen K
authors.initials: AK
authors.lastname: Mok
authors.forename: Philip S
authors.initials: PS
authors.lastname: Lukeman
mesh_terms: D004247:DNA / Q000737:chemistry*; D008956:Models, Chemical; D036103:Nanotechnology; D055668:Photochemical Processes
chemical_list: D004247:DNA