title: DNA barcoding and species boundary delimitation of selected species of Chinese Acridoidea (Orthoptera: Caelifera).
abstract: We tested the performance of DNA barcoding in Acridoidea and attempted to solve species boundary delimitation problems in selected groups using COI barcodes. Three analysis methods were applied to reconstruct the phylogeny. K2P distances were used to assess the overlap range between intraspecific variation and interspecific divergence. "Best match (BM)", "best close match (BCM)", "all species barcodes (ASB)" and "back-propagation neural networks (BP-based method)" were utilized to test the success rate of species identification. Phylogenetic species concept and network analysis were employed to delimitate the species boundary in eight selected species groups. The results demonstrated that the COI barcode region performed better in phylogenetic reconstruction at genus and species levels than at higher-levels, but showed a little improvement in resolving the higher-level relationships when the third base data or both first and third base data were excluded. Most overlaps and incorrect identifications may be due to imperfect taxonomy, indicating the critical role of taxonomic revision in DNA barcoding study. Species boundary delimitation confirmed the presence of oversplitting in six species groups and suggested that each group should be treated as a single species.
authors.affiliation: College of Life Sciences, Shaanxi Normal University, Xi'an, People's Republic of China ; College of Life Sciences, Guangxi Normal University, Guilin, People's Republic of China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Huang
authors.affiliation: College of Life Sciences, Capital Normal University, Beijing, People's Republic of China.
authors.forename: Aibing
authors.initials: A
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Shaanxi Normal University, Xi'an, People's Republic of China.
authors.forename: Shaoli
authors.initials: S
authors.lastname: Mao
authors.affiliation: College of Life Sciences, Shaanxi Normal University, Xi'an, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000818:Animals; D001483:Base Sequence; D001499:Bayes Theorem; D002681:China; D058893:DNA Barcoding, Taxonomic; D004272:DNA, Mitochondrial / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D014644:Genetic Variation; D006239:Haplotypes / Q000235:genetics; D008969:Molecular Sequence Data; D009987:Orthoptera / Q000145:classification*; D010802:Phylogeny; D016415:Sequence Alignment; D013045:Species Specificity
chemical_list: D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV