title: Minimizing artifactual elevation of lipid peroxidation products (F2-isoprostanes) in plasma during collection and storage.
abstract: F2-isoprostanes (F2-IsoP's) are reliable measures of in vivo lipid oxidation, but care is required to prevent artifactual elevation. We examined the effects of blood collection and storage on plasma F2-IsoP's. Blood was collected into EDTA/butylated hydroxytoluene/reduced glutathione (EDTA/BHT/GSH) or EDTA, at 4 °C or room temperature. Plasma was stored at -20 or -80 °C for 1 or 6 months before F2-IsoP's were assayed by GC-MS. The temperature of blood collection did not affect F2-IsoP's. However, storage at -20 °C or collection into EDTA resulted in significant increases in F2-IsoP's. Blood collection into EDTA/BHT/GSH and storage at -80 °C minimizes artifactual elevation of plasma F2-IsoP's.
authors.affiliation: School of Medicine & Pharmacology, Royal Perth Hospital, University of Western Australia, Perth, WA 6000, Australia. Electronic address: anne.barden@uwa.edu.au.
authors.forename: A E
authors.initials: AE
authors.lastname: Barden
authors.affiliation: School of Medicine & Pharmacology, Royal Perth Hospital, University of Western Australia, Perth, WA 6000, Australia.
authors.forename: E
authors.initials: E
authors.lastname: Mas
authors.affiliation: School of Medicine & Pharmacology, Royal Perth Hospital, University of Western Australia, Perth, WA 6000, Australia.
authors.forename: K D
authors.initials: KD
authors.lastname: Croft
authors.affiliation: West Australian Institute for Medical Research, University of Western Australia, Perth, WA 6000, Australia.
authors.forename: M
authors.initials: M
authors.lastname: Phillips
authors.affiliation: School of Medicine & Pharmacology, Royal Perth Hospital, University of Western Australia, Perth, WA 6000, Australia.
authors.forename: T A
authors.initials: TA
authors.lastname: Mori
mesh_terms: D001793:Blood Preservation; D002849:Chromatography, Gas; D003080:Cold Temperature; D028441:F2-Isoprostanes / Q000097:blood* / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation; D010949:Plasma / Q000378:metabolism
chemical_list: D028441:F2-Isoprostanes
keywords: Gas chromatography–mass spectrometry; Isoprostanes; Lipid peroxidation