title: [Separation performance of vancomycin-bonded stationary phase].
abstract: Vancomycin is one of the macrocyclic antibiotics with complex molecular structure. Based on the various functional groups of vancomycin, a vancomycin-bonded stationary phase using glutaraldehyde as the spacer was prepared. Its chromatographic properties in reversed-phase, ion exchange and hydrophilic phase modes were investigated separately. The prepared stationary phase showed the typical characteristic of the reversed-phase stationary, when the organic solvent content was low in the mobile phase. On the contrary, its chromatographic characteristic transformed into hydrophilic phase mode with the organic solvent content increased in the mobile phase. Owing to the amino groups of vancomycin, ion exchange mode can also be applied to the separation method development. The vancomycin-bonded stationary phase was applied to the separation of eight achiral drugs and stevioside in reversed-phase, ion exchange and hydrophilic phase modes. The separations were achieved in three different kinds of separation modes by using appropriate chromatographic conditions. The results provide guidance for the design of new types of stationary phase, and method development of chromatographic stationary phases modified by special compounds with complex construction in corresponding separation modes.
authors.affiliation: Department of Chemistry, Nanchang University, Nanchang 330031, China.
authors.forename: Lanqi
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Chemistry, Nanchang University, Nanchang 330031, China.
authors.forename: Weihu
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Chemistry, Nanchang University, Nanchang 330031, China.
authors.forename: Yiwang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Lingyi
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Nanchang University, Nanchang 330031, China.
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Wan
authors.affiliation: Department of Chemistry, Nanchang University, Nanchang 330031, China.
authors.forename: Weibing
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D057927:Hydrophobic and Hydrophilic Interactions; D015394:Molecular Structure; D014640:Vancomycin / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D014640:Vancomycin