title: Structural characterization of a novel glucan from Achatina fulica and its antioxidant activity.
abstract: A novel glucan designated AFPS-IB was purified from Achatina fulica (China white jade snail) by anion-exchange and gel-permeation chromatography. Chemical composition analysis indicated AFPS-IB was composed of glucose, fucose, rhamnose, mannose, and galactose in a molar ratio of 189:2:1:1:2 and with an average molecular weight of 128 kDa. Its structural characteristics were investigated by Fourier transform infrared spectroscopy (FTIR), high performance liquid chromatography (HPLC), gas chromatography mass spectrometry (GC-MS), methylation analysis, nuclear magnetic resonance (NMR) spectroscopy ((1)H,( 13)C, H-H COSY, HSQC, TOCSY, and NOESY), and atomic force microscopy (AFM). The glucan mainly consisted of a backbone of repeating (1→4)-α-d-glucose residues with (1→6)-β-d glucosyl branches at random points on the backbone glucose. Antioxidant studies revealed AFPS-IB showed significant DPPH (2,2-diphenyl-1-picrylhydrazyl) radical, superoxide anion (O2(-)) scavenging activities and high reduction potential. This study suggested that AFPS-IB could be a new source of dietary antioxidants.
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University , 866 Yuhangtang Road, Hangzhou, 310058 Zhejiang, China.
authors.forename: Ningbo
authors.initials: N
authors.lastname: Liao
authors.forename: Shiguo
authors.initials: S
authors.lastname: Chen
authors.forename: Xingqian
authors.initials: X
authors.lastname: Ye
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhong
authors.forename: Xuan
authors.initials: X
authors.lastname: Ye
authors.forename: Xinzi
authors.initials: X
authors.lastname: Yin
authors.forename: Jenny
authors.initials: J
authors.lastname: Tian
authors.forename: Donghong
authors.initials: D
authors.lastname: Liu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002681:China; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D015394:Molecular Structure; D012908:Snails / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D005936:Glucans