title: Gene structure, immune response and evolution: comparative analysis of three 2-Cys peroxiredoxin members of miiuy croaker, Miichthys miiuy.
abstract: Peroxiredoxin family was a superfamily of selenium independent peroxidases. It was divided into six subtypes: Prx1-4 (typical 2-Cys), Prx5 (atypical 2-Cys) and Prx6 (1-Cys). This study reports the isolation and characterization three 2-Cys peroxiredoxin members of full cDNA and genomic clones from miiuy croaker (Miichthys miiuy). The genetic structure analysis showed that the C-terminal catalytic Cys positioned within GEVCPAXW. This sequence was different between Prx3 and Prx4, but was conservative in different species of the same gene, the X base was S in Prx3 but G in Prx4. Tissues expression analysis showed that the expressions of Prx3 in liver and brain were much higher than other tissues; the values of Prx4 in spleen, intestine and kidney were significantly higher than others; and the expression of Prx5 in muscle was higher than that of other tissues. Real-time PCR results showed that there were highest values of these three Prxs emerging with the time post challenge of Vibrio anguillarum in liver, spleen and kidney although the highest value time differed from each other and the expression of these three genes also changed with the change of infection time. These results indicated that expression analysis of these three genes play some positive function against pathogenic bacteria infection in miiuy croaker.
authors.affiliation: Laboratory of Fish Biogenetics and Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan 316000, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Ren
authors.affiliation: Laboratory of Fish Biogenetics and Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan 316000, China.
authors.forename: Yuena
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Laboratory of Fish Biogenetics and Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan 316000, China. Electronic address: wangrixin1123@126.com.
authors.forename: Rixin
authors.initials: R
authors.lastname: Wang
authors.affiliation: Laboratory of Fish Biogenetics and Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan 316000, China. Electronic address: tianjunxu@163.com.
authors.forename: Tianjun
authors.initials: T
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D019143:Evolution, Molecular; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010473:Perciformes / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D054464:Peroxiredoxins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary; D014733:Vibrio / Q000502:physiology; D014735:Vibrio Infections / Q000276:immunology*
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger; D054464:Peroxiredoxins
keywords: Expression profiling; Gene structure; Miiuy croaker; Peroxiredoxin