title: Phenolic content, antibacterial and antioxidant activities of Erica herbacea L.
abstract: Antibacterial and antioxidant activity, total phenolic and flavonoid concentrations of aqueous, ethanol and ethyl acetate extracts from the leaves and flowers of Erica herbacea L. were studied. In vitro antibacterial activity of the extracts was determined by macrodilution method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) have been determined. Testing was performed on 30 clinical isolates, including different strains of Escherichia coli, Enterococcus faecalis and Proteus vulgaris. The values for MIC were in the range from 2.5 mg/mL to 40 mg/mL. The most sensitive bacterial strains were Proteus vulgaris strains. The aqueous extract from E. herbacea was found the most active. The total phenolic content was determined using Folin-Ciocalteu reagent and ranged between 14.98 and 119.88 mg GA/g. The concentration of flavonoids in extracts was determined using spectrophotometric method with aluminium chloride and obtained results varied from 16.19 to 26.90 mg RU/g. Antioxidant activity was monitored spectrophotometrically using DPPH reagent. The highest capacity to neutralize DPPH radicals was found in the aqueous extract from E. herbacea. The results of the total phenolic content determination of the examined extracts indicate that E. herbacea extracts are a rich source of phenolic compounds and also possess a significant antioxidant activity and moderate antibacterial activity.
authors.affiliation: Department of Microbiology, Medical Faculty, University of Banja Luka, Save Mrkalja 14, 78000 Banja Luka, Republic of Srpska, Bosnia and Herzegovina. draganavucicbl@yahoo.com
authors.forename: Dragana M
authors.initials: DM
authors.lastname: Vucić
authors.affiliation: Department of Microbiology, Medical Faculty, University of Banja Luka, Save Mrkalja 14, 78000 Banja Luka, Republic of Srpska, Bosnia and Herzegovina.
authors.forename: Miroslav R
authors.initials: MR
authors.lastname: Petković
authors.affiliation: Department of Organic Chemistry, Faculty of Technology, University of Banja Luka, Bulevar Stepe Stepanovića 73, 78000 Banja Luka, Republic of Srpska, Bosnia and Herzegovina.
authors.forename: Branka B
authors.initials: BB
authors.lastname: Rodić-Grabovac
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Republic of Serbia.
authors.forename: Olgica D
authors.initials: OD
authors.lastname: Stefanović
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Republic of Serbia.
authors.forename: Sava M
authors.initials: SM
authors.lastname: Vasić
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Republic of Serbia.
authors.forename: Ljiljana R
authors.initials: LR
authors.lastname: Comić
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D013293:Enterococcus faecalis / Q000187:drug effects / Q000254:growth & development; D029791:Ericaceae / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D035264:Flowers; D008826:Microbial Sensitivity Tests; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves; D010946:Plants, Medicinal; D011514:Proteus vulgaris / Q000187:drug effects / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl