title: Comprehensive analysis of HPV expression in laryngeal squamous cell carcinoma.
abstract: Role of human papillomavirus (HPV) in laryngeal carcinoma remains controversial. The aim of this study is to evaluate the role of HPV in laryngeal squamous cell carcinoma by determining presence of markers of viral infection. HPV DNA and E6*I mRNA status was determined by type-specific E7 PCR bead-based multiplex genotyping and RT-PCR assays in laryngeal squamous cell carcinoma biopsy samples. p16(INK4a) and COX-2 expression was determined by immunohistochemistry. Four cases out of 32 (13%) were HPV DNA+: HPV 11 (n = 1), HPV 31 (n = 3), HPV 59 (n = 1). One double infection: HPV 11 and HPV 31. p16(INK4a) was overexpressed in three cases (9%) and COX-2 in 17 cases (53%). Two of four HPV DNA+ samples had E6*I mRNA for HPV 31 and overexpressed p16(INK4a) and COX-2. HPV appears to play an active role in a small subset of laryngeal squamous cell carcinoma. p16(INK4a) can be used as a surrogate marker of transcriptionally active HPV infection; COX-2 expression had no correlation with HPV DNA and/or RNA positivity.
authors.affiliation: International Agency for Research on Cancer, Lyon, France.
authors.forename: Tarik
authors.initials: T
authors.lastname: Gheit
authors.forename: Behnoush
authors.initials: B
authors.lastname: Abedi-Ardekani
authors.forename: Christine
authors.initials: C
authors.lastname: Carreira
authors.forename: Carlos G
authors.initials: CG
authors.lastname: Missad
authors.forename: Massimo
authors.initials: M
authors.lastname: Tommasino
authors.forename: Mariela C
authors.initials: MC
authors.lastname: Torrente
mesh_terms: D000328:Adult; D000368:Aged; D052685:Alphapapillomavirus / Q000145:classification / Q000235:genetics; D014408:Biomarkers, Tumor; D002294:Carcinoma, Squamous Cell / Q000821:virology*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000096:biosynthesis / Q000235:genetics; D051546:Cyclooxygenase 2 / Q000096:biosynthesis / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005838:Genotype; D006258:Head and Neck Neoplasms / Q000821:virology*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000821:virology*; D008297:Male; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000235:genetics; D050725:Papillomavirus E7 Proteins / Q000235:genetics; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D012097:Repressor Proteins / Q000235:genetics; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D014408:Biomarkers, Tumor; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004279:DNA, Viral; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012367:RNA, Viral; D012097:Repressor Proteins; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human
keywords: COX-2; human papillomavirus; larynx cancer; p16INK4a