title: Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L.
abstract: Amoebic gill disease (AGD) is a disease caused by the ectoparasite Neoparamoeba perurans which affects several cultured marine fish worldwide. The characterisation of pro-inflammatory and immune related genes at the mRNA level in AGD-affected Atlantic salmon gills was performed at 10 days post-inoculation using 2D quantitative RT-PCR, a method of mapping transcriptional responses in tissues. The genes of interest were IL-1β, TNF-α, TCR-α chain, CD8, CD4, MHC-IIα, MHC-I, IgM and IgT. A significant increase in expression of the mRNA of all the genes was observed in the gills of AGD-affected fish. Contrary to previous studies, our data suggest that the parasite, N. perurans, elicits a classical inflammatory response in the gills of AGD-affected fish and indicates that the mRNA expression of immune genes within gill lesions misrepresents the cellular immune response in the gills during AGD.
authors.affiliation: National Centre for Marine Conservation and Resource Sustainability, Australian Maritime College, University of Tasmania, Launceston, Australia.
authors.forename: Y
authors.initials: Y
authors.lastname: Pennacchi
authors.affiliation: National Centre for Marine Conservation and Resource Sustainability, Australian Maritime College, University of Tasmania, Launceston, Australia.
authors.forename: M J
authors.initials: MJ
authors.lastname: Leef
authors.affiliation: National Centre for Marine Conservation and Resource Sustainability, Australian Maritime College, University of Tasmania, Launceston, Australia.
authors.forename: P B B
authors.initials: PB
authors.lastname: Crosbie
authors.affiliation: National Centre for Marine Conservation and Resource Sustainability, Australian Maritime College, University of Tasmania, Launceston, Australia.
authors.forename: B F
authors.initials: BF
authors.lastname: Nowak
authors.affiliation: National Centre for Marine Conservation and Resource Sustainability, Australian Maritime College, University of Tasmania, Launceston, Australia. Electronic address: Andrew.Bridle@utas.edu.au.
authors.forename: A R
authors.initials: AR
authors.lastname: Bridle
mesh_terms: D000562:Amebiasis / Q000276:immunology / Q000662:veterinary*; D056894:Amoebozoa / Q000502:physiology*; D000818:Animals; D005393:Fish Diseases / Q000276:immunology*; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005880:Gills / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D020317:Salmo salar
chemical_list: D029941:Fish Proteins; D012333:RNA, Messenger
keywords: 2D quantitative RT-PCR, inflammatory response; AGD; Atlantic salmon; mRNA gene expression