title: Ipl1/Aurora kinase suppresses S-CDK-driven spindle formation during prophase I to ensure chromosome integrity during meiosis.
abstract: Cells coordinate spindle formation with DNA repair and morphological modifications to chromosomes prior to their segregation to prevent cell division with damaged chromosomes. Here we uncover a novel and unexpected role for Aurora kinase in preventing the formation of spindles by Clb5-CDK (S-CDK) during meiotic prophase I and when the DDR is active in budding yeast. This is critical since S-CDK is essential for replication during premeiotic S-phase as well as double-strand break induction that facilitates meiotic recombination and, ultimately, chromosome segregation. Furthermore, we find that depletion of Cdc5 polo kinase activity delays spindle formation in DDR-arrested cells and that ectopic expression of Cdc5 in prophase I enhances spindle formation, when Ipl1 is depleted. Our findings establish a new paradigm for Aurora kinase function in both negative and positive regulation of spindle dynamics.
authors.affiliation: MRC Genome Damage and Stability Centre, University of Sussex, Brighton, United Kingdom.
authors.forename: Louise
authors.initials: L
authors.lastname: Newnham
authors.affiliation: MRC Genome Damage and Stability Centre, University of Sussex, Brighton, United Kingdom.
authors.forename: Philip W
authors.initials: PW
authors.lastname: Jordan
authors.affiliation: MRC Genome Damage and Stability Centre, University of Sussex, Brighton, United Kingdom.
authors.forename: Jesus A
authors.initials: JA
authors.lastname: Carballo
authors.affiliation: MRC Genome Damage and Stability Centre, University of Sussex, Brighton, United Kingdom.
authors.forename: Sonya
authors.initials: S
authors.lastname: Newcombe
authors.affiliation: MRC Genome Damage and Stability Centre, University of Sussex, Brighton, United Kingdom.
authors.forename: Eva
authors.initials: E
authors.lastname: Hoffmann
mesh_terms: D064127:Aurora Kinases / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D015825:Chromosomes, Fungal / Q000235:genetics / Q000378:metabolism*; D019926:Cyclin B / Q000378:metabolism*; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000378:metabolism; D049469:Meiotic Prophase I; D009154:Mutation; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: C076558:CLB5 protein, S cerevisiae; D018797:Cell Cycle Proteins; D019926:Cyclin B; D004268:DNA-Binding Proteins; C086672:HOP1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D064127:Aurora Kinases; C576961:IPL1 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases; C081927:CDC5 protein, S cerevisiae