title: Expression of B-cell-specific Moloney murine leukemia virus integration site 1 mRNA and protein in gastric cancer.
abstract: OBJECTIVE
  To investigate the role of B-cell-specific Moloney murine leukemia virus integration site 1 (Bmi-1) in gastric cancer (GC) and its relationship with the clinicopathological features of GC.
  
  
  METHODS
  Laser capture microdissection combined with real-time polymerase chain reaction and Western blot were used to determine the expressions of Bmi-1, the cellular homologue of avian myelocytomatosis virus (c-Myc), enhancer of Zeste homolog 2 (EZH2), phosphatase and tensin homologue (PTEN) and epithelial cadherin (E-cadherin) in 20 GC specimens and the adjacent non-cancerous gastric tissues.
  
  
  RESULTS
  The mRNA and protein expressions of Bmi-1 in GC were increased compared with those of the non-cancerous gastric tissues (P = 0.012 and P = 0.000, respectively). Bmi-1 mRNA expression was positively correlated with tumor size, degree of tumor differentiation, invasion and lymph node metastasis. At both mRNA and protein levels, Bmi-1 was positively correlated with c-Myc and EZH2, but negatively correlated with PTEN and E-cadherin.
  
  
  CONCLUSIONS
  Bmi-1 might be involved in GC at both transcription and translation levels.
authors.affiliation: Department of Gastroenterology and Hepatology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Division of Gastrointestinal Pathology, Shanghai Institute of Digestive Disease, Shanghai, China.
authors.forename: Peng Wei
authors.initials: PW
authors.lastname: Liu
authors.forename: Yun
authors.initials: Y
authors.lastname: Lin
authors.forename: Xiao Yu
authors.initials: XY
authors.lastname: Chen
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology / Q000556:secondary; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D061346:Laser Capture Microdissection / Q000379:methods; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D009367:Neoplasm Staging; D063150:Polycomb Repressive Complex 1 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: C069077:BMI1 protein, human; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D063150:Polycomb Repressive Complex 1
keywords: Bmi-1 protein; gene expression; laser capture microdissection; neoplasm metastasis; neoplasm staging; stomach neoplasm