title: The effect of elevated temperature on Barley yellow dwarf virus-PAV in wheat.
abstract: Barley yellow dwarf virus-PAV (BYDV-PAV) is associated with yellow dwarf disease, one of the most economically important diseases of cereals worldwide. In this study, the impact of current and future predicted temperatures for the Wimmera wheat growing district in Victoria, Australia on the titre of BYDV-PAV in wheat was investigated. Ten-day old wheat (Triticum aestivum, cv. Yitpi) seedlings were inoculated with BYDV-PAV and grown at ambient (5.0-16.1°C, night-day) or elevated (10.0-21.1°C, night-day) temperature treatments, simulating the current Wimmera average and future daily temperature cycles, respectively, during the wheat-growing season. Whole above-ground plant samples were collected from each temperature treatment at 0 (day of inoculation), 3, 6, 9, 12, 15, 18, 21 and 24 days after inoculation and the titre of BYDV-PAV was measured in each sample using a specific one-step multiplex normalised reverse transcription quantitative PCR (RT-qPCR) assay. Physical measurements, including plant height, dry weight and tiller number, were also taken at each sampling point. The titre of BYDV-PAV was significantly greater in plants grown in the elevated temperature treatment than in plants grown in the ambient treatment on days 6, 9 and 12. Plants grown at elevated temperature were significantly bigger and symptoms associated with BYDV-PAV were visible earlier than in plants grown at ambient temperature. These results may have important implications for the epidemiology of yellow dwarf disease under future climates in Australia.
authors.affiliation: Department of Environment and Primary Industries, AgriBio, 5 Ring Road, Bundoora, Victoria 3083, Australia. Electronic address: narelle.nancarrow@depi.vic.gov.au.
authors.forename: Narelle
authors.initials: N
authors.lastname: Nancarrow
authors.affiliation: Department of Environment and Primary Industries, AgriBio, 5 Ring Road, Bundoora, Victoria 3083, Australia.
authors.forename: Fiona E
authors.initials: FE
authors.lastname: Constable
authors.affiliation: Department of Environment and Primary Industries, AgriBio, 5 Ring Road, Bundoora, Victoria 3083, Australia.
authors.forename: Kyla J
authors.initials: KJ
authors.lastname: Finlay
authors.affiliation: Department of Environment and Primary Industries, Horsham Centre, 110 Natimuk Road, Horsham, Victoria 3400, Australia.
authors.forename: Angela J
authors.initials: AJ
authors.lastname: Freeman
authors.affiliation: Department of Environment and Primary Industries, AgriBio, 5 Ring Road, Bundoora, Victoria 3083, Australia.
authors.forename: Brendan C
authors.initials: BC
authors.lastname: Rodoni
authors.affiliation: Department of Environment and Primary Industries, Horsham Centre, 110 Natimuk Road, Horsham, Victoria 3400, Australia.
authors.forename: Piotr
authors.initials: P
authors.lastname: Trebicki
authors.affiliation: Department of Botany, La Trobe University, Bundoora, Victoria 3083, Australia.
authors.forename: Simone
authors.initials: S
authors.lastname: Vassiliadis
authors.affiliation: Department of Environment and Primary Industries, AgriBio, 5 Ring Road, Bundoora, Victoria 3083, Australia.
authors.forename: Alan L
authors.initials: AL
authors.lastname: Yen
authors.affiliation: Department of Environment and Primary Industries, AgriBio, 5 Ring Road, Bundoora, Victoria 3083, Australia; Plant Biosecurity CRC, The University of Melbourne, Burnley Campus, 500 Yarra Boulevard, Richmond, Victoria 3121, Australia.
authors.forename: Jo E
authors.initials: JE
authors.lastname: Luck
mesh_terms: D000818:Animals; D001042:Aphids / Q000821:virology; D001315:Australia; D007303:Insect Vectors / Q000821:virology; D017793:Luteovirus / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D015233:Models, Statistical; D010935:Plant Diseases / Q000469:parasitology / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature; D014908:Triticum / Q000469:parasitology / Q000821:virology*; D019562:Viral Load
chemical_list: D012367:RNA, Viral
keywords: Barley yellow dwarf virus-PAV; Climate change; Elevated temperature; Triticum aestivum; Virus titre; Wheat