title: Efficient asymmetric synthesis of tryptophan analogues having useful photophysical properties.
abstract: Two new fluorescent probes of protein structure and dynamics have been prepared by concise asymmetric syntheses using the Schöllkopf chiral auxiliary. The site-specific incorporation of one probe into dihydrofolate reductase is reported. The utility of these tryptophan derivatives lies in their absorption and emission maxima which differ from those of tryptophan, as well as in their large Stokes shifts and high molar absorptivities.
authors.affiliation: Center for BioEnergetics, The Biodesign Institute, and Department of Chemistry and Biochemistry, Arizona State University , Tempe, Arizona 85827, United States.
authors.forename: Poulami
authors.initials: P
authors.lastname: Talukder
authors.forename: Shengxi
authors.initials: S
authors.lastname: Chen
authors.forename: Pablo M
authors.initials: PM
authors.lastname: Arce
authors.forename: Sidney M
authors.initials: SM
authors.lastname: Hecht
mesh_terms: D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D055668:Photochemical Processes; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D014364:Tryptophan