title: Proteomics of larval hemolymph in Bombyx mori reveals various nutrient-storage and immunity-related proteins.
abstract: The silkworm, Bombyx mori, is an important economic insect for its production of silk. The larvae of many lepidopteran insects are major agricultural pests and often silkworm is explored as a model organism for other lepidopteran pest species. The hemolymph of caterpillars contains a lot of nutrient and immune components. In this study, we applied liquid chromatography-tandem mass spectrometry to gain a better understanding of the larval hemolymph proteomics in B. mori. We identified 752 proteins in hemolymph collected from day-4 fourth instar and day-7 fifth instar. Nearly half the identified proteins (49%) were predicted to function as binding proteins and 46% were predicted to have catalytic activities. Apolipophorins, storage proteins, and 30K proteins constituted the most abundant groups of nutrient-storage proteins. Of them, 30K proteins showed large differences between fourth instar larvae and fifth instar larvae. Besides nutrient-storage proteins, protease inhibitors are also expressed very highly in hemolymph. The analysis also revealed lots of immunity-related proteins, including recognition, signaling, effectors and other proteins, comprising multiple immunity pathways in hemolymph. Our data provide an exhaustive research of nutrient-storage proteins and immunity-related proteins in larval hemolymph, and will pave the way for future physiological and pathological studies of caterpillars.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhaoming
authors.initials: Z
authors.lastname: Dong
authors.forename: Dandan
authors.initials: D
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Wu
authors.forename: Qianru
authors.initials: Q
authors.lastname: Song
authors.forename: Peiming
authors.initials: P
authors.lastname: Gu
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
authors.forename: Qingyou
authors.initials: Q
authors.lastname: Xia
mesh_terms: D000818:Animals; D012831:Bombyx / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D006458:Hemolymph / Q000737:chemistry / Q000276:immunology*; D019476:Insect Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D007814:Larva / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development / Q000276:immunology; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D040901:Proteomics
chemical_list: D019476:Insect Proteins; C041137:storage proteins, Insecta