title: Decreased expression of proteins involved in energy metabolism in the hippocampal granular layer of rats submitted to the pilocarpine epilepsy model.
abstract: Long-term structural and functional changes in the hippocampus have been identified as the primary physiopathological basis for temporal lobe epilepsy. These changes include reactive gliosis and granule cell axonal sprouting within the dentate gyrus. The intimate mechanisms of these changes are beginning to be revealed. Here, we show the possibility of using laser capture microdissection (LCM) to isolate the dentate granular cell layer of Wistar rats submitted to the pilocarpine model of epilepsy. Using two-dimensional gel electrophoresis (2-D PAGE) and mass spectrometry for laser-captured cells, we identified molecular events that could be altered as part of the epileptic pathogenic process. According to our results, eight proteins related to energy metabolism were differentially expressed between both the control and pilocarpine-treated animals. These results provide, for the first time, new molecular insights into the altered protein profile of the epileptogenic dentate gyrus and can contribute to a better understanding of the phenomena involved in the genesis and maintenance of the epileptic state.
authors.affiliation: Neurologia Experimental - Escola Paulista de Medicina/Universidade Federal de São Paulo, São Paulo, SP, Brazil; University of California San Diego, School of Medicine, Department of Pediatrics/Rady Children's Hospital San Diego, Department of Cellular & Molecular Medicine, Stem Cell Program, La Jolla, CA, USA. Electronic address: bhsa83@gmail.com.
authors.forename: Bruno
authors.initials: B
authors.lastname: Araujo
authors.affiliation: Neurologia Experimental - Escola Paulista de Medicina/Universidade Federal de São Paulo, São Paulo, SP, Brazil; Hospital Israelita Albert Einstein - Instituto do Cérebro, São Paulo, SP, Brazil; University of California San Diego, School of Medicine, Department of Pediatrics/Rady Children's Hospital San Diego, Department of Cellular & Molecular Medicine, Stem Cell Program, La Jolla, CA, USA.
authors.forename: Laila
authors.initials: L
authors.lastname: Torres
authors.affiliation: Laboratório de Biologia Molecular de Parasitas - Escola Paulista de Medicina/Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Mariana
authors.initials: M
authors.lastname: Stein
authors.affiliation: Hospital Israelita Albert Einstein - Instituto do Cérebro, São Paulo, SP, Brazil; Faculdade de Ciências Médicas da Santa Casa de São Paulo, São Paulo, SP, Brazil.
authors.forename: Francisco Romero
authors.initials: FR
authors.lastname: Cabral
authors.affiliation: University of California San Diego, School of Medicine, Department of Pediatrics/Rady Children's Hospital San Diego, Department of Cellular & Molecular Medicine, Stem Cell Program, La Jolla, CA, USA.
authors.forename: Roberto
authors.initials: R
authors.lastname: Herai
authors.affiliation: Departamento de Genética e Biologia Evolutiva, Universidade de São Paulo, USP, Instituto de Biociências, São Paulo, SP, Brazil.
authors.forename: Oswaldo
authors.initials: O
authors.lastname: Okamoto
authors.affiliation: Neurologia Experimental - Escola Paulista de Medicina/Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Esper
authors.initials: E
authors.lastname: Cavalheiro
mesh_terms: D000818:Animals; D004734:Energy Metabolism; D004827:Epilepsy / Q000139:chemically induced / Q000378:metabolism*; D004833:Epilepsy, Temporal Lobe / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D010862:Pilocarpine; D011506:Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D017208:Rats, Wistar
chemical_list: D011506:Proteins; D020543:Proteome; D010862:Pilocarpine
keywords: Energy metabolism; Laser capture microdissection; Temporal lobe epilepsy; Two-dimensional gel electrophoresis