title: Ionic polymeric micelles based on chitosan and fatty acids and intended for wound healing. Comparison of linoleic and oleic acid.
abstract: Chitosan is well known for its positive properties in wound healing. Also unsaturated fatty acids are described as able to accelerate tissue repairing mechanisms. In this work hydrophobically modified chitosan was obtained by ionic interaction with either oleic or linoleic acid. In aqueous environment self-assembling into nanoparticles occurred. The presence of hydrophobic domains, similar to those present in polymeric micelles, was demonstrated by changes in pyrene spectra. Both oleate and linoleate derivatives showed mucoadhesion behaviour. Cytotoxicity tests on human dermal fibroblasts demonstrated good biocompatibility of especially oleate derivatives. Clarithromycin, a poorly soluble model drug proposed for use in infected wounds was successfully encapsulated in both oleic and linoleic based polymeric micelles. The ionic structure of the carriers is responsible for their loosening at neutral pH and in the presence of salts. This behaviour should impair parenteral administration of the systems, but can be useful for topical delivery where the micelle components, chitosan and fatty acid, can play a positive role in dermal regeneration and tissue repairing.
authors.affiliation: University of Pavia, Department of Drug Sciences, Pavia, Italy. Electronic address: cbonferoni@unipv.it.
authors.forename: M C
authors.initials: MC
authors.lastname: Bonferoni
authors.affiliation: University of Pavia, Department of Drug Sciences, Pavia, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Sandri
authors.affiliation: University of Pavia, Department of Drug Sciences, Pavia, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Dellera
authors.affiliation: University of Pavia, Department of Drug Sciences, Pavia, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Rossi
authors.affiliation: University of Pavia, Department of Drug Sciences, Pavia, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Ferrari
authors.affiliation: University of Pavia, Department of Drug Sciences, Pavia, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Mori
authors.affiliation: University of Pavia, Department of Drug Sciences, Pavia, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Caramella
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D017291:Clarithromycin / Q000008:administration & dosage / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D004339:Drug Compounding; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D019787:Linoleic Acid / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D008823:Micelles; D053758:Nanoparticles; D019301:Oleic Acid / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D010316:Particle Size; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D008823:Micelles; D019301:Oleic Acid; D048271:Chitosan; D019787:Linoleic Acid; D017291:Clarithromycin
keywords: Clarithromycin; Hydrophobically modified chitosan; Ionic interaction; Polymeric micelles; Unsaturated fatty acids; Wound healing