title: Bone regeneration of mouse critical-sized calvarial defects with human mesenchymal stem cells in scaffold.
abstract: Combination of tissue engineering and cell therapy represents a promising approach for bone regeneration. Human mesenchymal stem cells (hMSCs) have properties that include low immunogenicity, high proliferation rate, and multi-differentiation potential; therefore, they are an attractive seeding source for tissue engineering therapy. Here we found that hMSCs with a scaffold did not affect cell viability and osteogenic differentiation. We also investigated regenerative effect of hMSCs with the scaffold in a calvarial bone defect model. Formation of new bone was evaluated by micro-CT, histology and expression of osteogenic markers. The results clearly showed interesting evidence indicating that hMSCs with scaffold increased the formation of new bone and expression of osteogenic markers, compared to the empty and scaffold only groups. Overall, our results suggest that hMSCs with scaffold are suitable for stimulation of intense bone regeneration in critical-sized bone defects.
authors.affiliation: Stem Cell Neuroplasticity Research Group, Kyungpook National University, Daegu, Korea. ; Department of Physiology, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu, Korea.
authors.forename: Jin-Young
authors.initials: JY
authors.lastname: Im
authors.affiliation: Department of Orthopaedic Surgery, Kyungpook National University Hospital, Daegu, Korea.
authors.forename: Woo-Kie
authors.initials: WK
authors.lastname: Min
authors.affiliation: Ossgen, Gyeongbuk Technopark, Gyeongbuk, Korea.
authors.forename: Changkook
authors.initials: C
authors.lastname: You
authors.affiliation: Department of Laboratory Medicine, Yonsei Cell Therapy Center, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Hyun-Ok
authors.initials: HO
authors.lastname: Kim
authors.affiliation: Stem Cell Neuroplasticity Research Group, Kyungpook National University, Daegu, Korea. ; Department of Laboratory Animal Medicine, College of Veterinary Medicine, Kyungpook National University, Daegu, Korea.
authors.forename: Hee-Kyung
authors.initials: HK
authors.lastname: Jin
authors.affiliation: Stem Cell Neuroplasticity Research Group, Kyungpook National University, Daegu, Korea. ; Department of Physiology, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu, Korea.
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Bae
keywords: Human mesenchymal stem cells; bone regeneration; calvarial bone defect; scaffold