title: Ultra high-throughput single molecule spectroscopy with a 1024 pixel SPAD.
abstract: Single-molecule spectroscopy is a powerful approach to measuring molecular properties such as size, brightness, conformation, and binding constants. Due to the low concentrations in the single-molecule regime, measurements with good statistical accuracy require long acquisition times. Previously we showed a factor of 8 improvement in acquisition speed using a custom-CMOS 8x1 SPAD array. Here we present preliminary results with a 64X improvement in throughput obtained using a liquid crystal on silicon spatial light modulator (LCOS-SLM) and a novel standard CMOS 1024 pixel SPAD array, opening the way to truly high-throughput single-molecule spectroscopy.
authors.affiliation: Department of Chemistry & Biochemistry, UCLA, Los Angeles, CA.
authors.forename: Ryan A
authors.initials: RA
authors.lastname: Colyer
authors.affiliation: Department of Chemistry & Biochemistry, UCLA, Los Angeles, CA.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Scalia
authors.affiliation: Dipartimento di Elettronica ed Informazione, Politecnico di Milano, Milano, Italy.
authors.forename: Federica A
authors.initials: FA
authors.lastname: Villa
authors.affiliation: Dipartimento di Elettronica ed Informazione, Politecnico di Milano, Milano, Italy.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Guerrieri
authors.affiliation: Micro Photon Devices, Bolzano, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Tisa
authors.affiliation: Dipartimento di Elettronica ed Informazione, Politecnico di Milano, Milano, Italy.
authors.forename: Franco
authors.initials: F
authors.lastname: Zappa
authors.affiliation: Dipartimento di Elettronica ed Informazione, Politecnico di Milano, Milano, Italy.
authors.forename: Sergio
authors.initials: S
authors.lastname: Cova
authors.affiliation: Department of Chemistry & Biochemistry, UCLA, Los Angeles, CA.
authors.forename: Shimon
authors.initials: S
authors.lastname: Weiss
authors.affiliation: Department of Chemistry & Biochemistry, UCLA, Los Angeles, CA.
authors.forename: Xavier
authors.initials: X
authors.lastname: Michalet
keywords: CMOS; FCS; LCOS; SPAD array; fluorescence; high-throughput; photon counting; single-molecule