title: Perception of the novel MAMP eMax from different Xanthomonas species requires the Arabidopsis receptor-like protein ReMAX and the receptor kinase SOBIR.
abstract: As part of their innate immune system plants carry a number of pattern recognition receptors (PRRs) that can detect a broad range of microbe-associated molecular patterns (MAMPs). In a recently published article (1) we described a novel, proteinaceous MAMP termed eMax (enigmatic MAMP of Xanthomonas) that derives from Xanthomonas and gets recognized by the receptor-like protein ReMAX (RECEPTOR OF eMax) of Arabidopsis thaliana. ReMAX has no ortholog in Nicotiana benthamiana and this species does not respond to eMax even when transformed with ReMAX. However, interfamily transfer of eMax perception was successful with a chimeric form of ReMAX where the C-terminal part of the protein was replaced by the corresponding part of the tomato RLP EIX2 (ETHYLENE INDUCING XYLANASE2). In this addendum we describe the difficulties with the purification and identification of the MAMP eMax and we present data demonstrating that functionality of ReMAX, much like that of related RLPs, depends on the presence of the receptor kinase SOBIR (SUPPRESSOR OF BIR1-1).
authors.affiliation: Zentrum für Molekularbiologie der Pflanzen; University Tübingen; Tuebingen, Germany.
authors.forename: Anna Kristina
authors.initials: AK
authors.lastname: Jehle
authors.affiliation: Zentrum für Molekularbiologie der Pflanzen; University Tübingen; Tuebingen, Germany.
authors.forename: Ursula
authors.initials: U
authors.lastname: Fürst
authors.affiliation: Zentrum für Molekularbiologie der Pflanzen; University Tübingen; Tuebingen, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Lipschis
authors.affiliation: Zentrum für Molekularbiologie der Pflanzen; University Tübingen; Tuebingen, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Albert
authors.affiliation: Zentrum für Molekularbiologie der Pflanzen; University Tübingen; Tuebingen, Germany.
authors.forename: Georg
authors.initials: G
authors.lastname: Felix
mesh_terms: D017360:Arabidopsis / Q000378:metabolism* / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D005030:Ethylenes / Q000096:biosynthesis; D005408:Flagellin / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D011494:Protein Kinases / Q000378:metabolism*; D051192:Receptors, Pattern Recognition / Q000378:metabolism*; D014974:Xanthomonas / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D005030:Ethylenes; C586779:RLP1 protein, Arabidopsis; D051192:Receptors, Pattern Recognition; D005408:Flagellin; C036216:ethylene; D011494:Protein Kinases; C000593371:SOBIR protein, Arabidopsis
keywords: Arabidopsis thaliana; Xanthomonas; microbe-associated molecular pattern; pattern recognition receptor; plant innate immunity