title: Large-scale mapping of transposable element insertion sites using digital encoding of sample identity.
abstract: Determining the genomic locations of transposable elements is a common experimental goal. When mapping large collections of transposon insertions, individualized amplification and sequencing is both time consuming and costly. We describe an approach in which large numbers of insertion lines can be simultaneously mapped in a single DNA sequencing reaction by using digital error-correcting codes to encode line identity in a unique set of barcoded pools.
authors.affiliation: Department of Neurobiology, Stanford University, Stanford, California 94305.
authors.forename: Daryl M
authors.initials: DM
authors.lastname: Gohl
authors.forename: Limor
authors.initials: L
authors.lastname: Freifeld
authors.forename: Marion
authors.initials: M
authors.lastname: Silies
authors.forename: Jennifer J
authors.initials: JJ
authors.lastname: Hwa
authors.forename: Mark
authors.initials: M
authors.lastname: Horowitz
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Clandinin
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D049750:Genome, Insect; D023281:Genomics / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing; D017422:Sequence Analysis, DNA
chemical_list: D004251:DNA Transposable Elements
keywords: Drosophila melanogaster; mapping; next-generation sequencing; transposable elements