title: Induced marine fungus Chondrostereum sp. as a means of producing new sesquiterpenoids chondrosterins I and J by using glycerol as the carbon source.
abstract: Chondrostereum sp., a marine fungus isolated from a soft coral Sarcophyton tortuosum, can yield hirsutane framework sesquiterpenoids. However, the metabolites profiles vary dramatically with the composition change of the culture media. This fungus was cultured in a liquid medium containing glycerol as the carbon source, and two new metabolites, chondrosterins I and J (1 and 2), were obtained. Their structures were elucidated primarily based on MS, NMR and X-ray single-crystal diffraction data. By comparison with the known hirsutane sesquiterpenoids, chondrosterins I and J have unique structural features, including a methyl was migrated from C-2 to C-6, and the methyl at C-3 was carboxylated. Compound 2 exhibited potent cytotoxic activities against the cancer cell lines CNE-1 and CNE-2 with the IC50 values of 1.32 and 0.56 μM.
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China. ceslhj@mail.sysu.edu.cn.
authors.forename: Hou-Jin
authors.initials: HJ
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China. natprodjwh@gmail.com.
authors.forename: Wen-Han
authors.initials: WH
authors.lastname: Jiang
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China. natprodlwl@gmail.com.
authors.forename: Wan-Ling
authors.initials: WL
authors.lastname: Liang
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China. natprodhjx1@gmail.com.
authors.forename: Jia-Xin
authors.initials: JX
authors.lastname: Huang
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China. marinemyf@yahoo.com.
authors.forename: Yu-Fei
authors.initials: YF
authors.lastname: Mo
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China. marinedyq@gmail.com.
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Ding
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China. cklam@mail.sysu.edu.cn.
authors.forename: Chi-Keung
authors.initials: CK
authors.lastname: Lam
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China. marineqxj@gmail.com.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Qian
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China. zhuxfeng@mail.sysu.edu.cn.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Zhu
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China. lanwj@mail.sysu.edu.cn.
authors.forename: Wen-Jian
authors.initials: WJ
authors.lastname: Lan
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D018360:Crystallography, X-Ray; D003470:Culture Media; D004354:Drug Screening Assays, Antitumor; D005990:Glycerol / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D011124:Polyporaceae / Q000737:chemistry / Q000378:metabolism*; D012717:Sesquiterpenes / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000970:Antineoplastic Agents; D003470:Culture Media; D012717:Sesquiterpenes; D013778:Tetrazolium Salts; D013844:Thiazoles; C000590751:chondrosterin J; C022616:thiazolyl blue; D005990:Glycerol