title: The effect of hypoxia and reoxygenation in the response of mesangial cells to angiotensin II in vitro.
abstract: BACKGROUND
  Mesangial cells (MC) may be involved in the glomerular alterations induced by ischemia/reperfusion injury.
  
  
  OBJECTIVE
  To evaluate the response of immortalized MC (IMC) to 30 minutes of hypoxia followed by reoxygenation periods of 30 minutes (H/R30) or 24 hours (H/R24).
  
  
  METHODS
  The intracellular calcium concentration ([Ca+2]i) was measured before (baseline) and after adding angiotensin II (AII, 10-5 M) in the presence and absence of glybenclamide (K ATP channel blocker). We estimated the level of intracellular ATP, nitric oxide (NO) and PGE2.
  
  
  RESULTS
  ATP concentration decreased after hypoxia and increased after reoxygenation. Hypoxia and H/R induced increases in basal [Ca+2]i. AII induced increases in [Ca+2]i in normoxia (97 ± 9%), hypoxia (72 ± 10%) or HR30 (85 ± 17%) groups, but there was a decrease in the response to AII in group H/R24 since the elevation in [Ca+2]i was significantly lower than in control (61 ± 10%, p < 0.05). Glybenclamide did not modify this response. It was observed a significant increase in NO generation after 24 hours of reoxygenation, but no difference in PGE2 production was observed. Data suggest that H/R injury is characterized by increased basal [Ca+2]i and by an impairment in the response of cells to AII. Results suggest that the relative insensibility to AII may be at least in part mediated by NO but not by prostaglandins or vasodilator K ATP channels.
  
  
  CONCLUSIONS
  H/R caused dysfunction in IMC characterized by increases in basal [Ca+2]i during hypoxia and reduction in the functional response to AII during reoxygenation.
authors.forename: Clara Versolato
authors.initials: CV
authors.lastname: Razvickas
authors.forename: Fernanda Teixeira
authors.initials: FT
authors.lastname: Borges
authors.forename: Andréia Silva
authors.initials: AS
authors.lastname: de Oliveira
authors.forename: Nestor
authors.initials: N
authors.lastname: Schor
authors.forename: Mirian Aparecida
authors.initials: MA
authors.lastname: Boim
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology* / Q000502:physiology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D015687:Cell Hypoxia; D002478:Cells, Cultured; D050527:Mesangial Cells / Q000187:drug effects* / Q000502:physiology*; D051379:Mice; D010100:Oxygen / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000804:Angiotensin II; D010100:Oxygen; D002118:Calcium