title: Technological aptitude and applications of Leuconostoc mesenteroides bioactive strains isolated from Algerian raw camel milk.
abstract: Two strains (B7 and Z8) of the Leuconostoc mesenteroides subspecies mesenteroides that were isolated from Algerian camel milk from an initial pool of 13 strains and demonstrated a high ability to inhibit the growth of Listeria spp. were selected and characterised at the phenotypic and genotypic levels. Probiotic profiling and inhibition spectra against food borne pathogens in mixed cultures were also investigated. The bacteriocin produced by L. mesenteroides strain B7 was identified as leucocin B by specific PCR. In vitro studies demonstrated that both Leuconostoc mesenteroides strains exhibited a marked probiotic profile, showing high survival at low pH (2-3 and 4) in the presence of 0.5%, 1%, and 2% of bile salts and at pH 3 in the presence of 3 mg/mL pepsin. Susceptibility testing against antimicrobial agents was also performed for both strains. When tested in a mixed culture with Listeria innocua, Listeria ivanovii, or Staphylococcus aureus, strain B7 reduced the numbers of these species by 1.87, 1.78, and 1.38 log units, respectively. Consequently, these two strains were found to possess good probiotic properties in vitro and a high capacity for Listeria spp. inhibition in mixed cultures. Therefore, these strains have a favourable technological aptitude and a potential application as novel probiotic starters.
authors.affiliation: Laboratory of Applied Microbiology, Department of Biology, Faculty of Sciences, Oran University, B.P. 16, Es-Senia, 31100 Oran, Algeria.
authors.forename: Zineb
authors.initials: Z
authors.lastname: Benmechernene
authors.affiliation: Laboratory of Applied Microbiology, Department of Biology, Faculty of Sciences, Oran University, B.P. 16, Es-Senia, 31100 Oran, Algeria.
authors.forename: Hanane Fatma
authors.initials: HF
authors.lastname: Chentouf
authors.affiliation: Laboratory of Applied Microbiology, Department of Biology, Faculty of Sciences, Oran University, B.P. 16, Es-Senia, 31100 Oran, Algeria.
authors.forename: Bellil
authors.initials: B
authors.lastname: Yahia
authors.affiliation: Laboratory of Applied Microbiology, Department of Biology, Faculty of Sciences, Oran University, B.P. 16, Es-Senia, 31100 Oran, Algeria.
authors.forename: Ghazi
authors.initials: G
authors.lastname: Fatima
authors.affiliation: Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences/College of Biotechnology, University of Santiago de Compostela, Rúa Carballo Calero s/n, Campus Lugo, 27002 Lugo, Spain.
authors.forename: Marcos
authors.initials: M
authors.lastname: Quintela-Baluja
authors.affiliation: Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences/College of Biotechnology, University of Santiago de Compostela, Rúa Carballo Calero s/n, Campus Lugo, 27002 Lugo, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Calo-Mata
authors.affiliation: Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences/College of Biotechnology, University of Santiago de Compostela, Rúa Carballo Calero s/n, Campus Lugo, 27002 Lugo, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: Barros-Velázquez
mesh_terms: D000818:Animals; D001430:Bacteriocins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002162:Camelus / Q000382:microbiology*; D005516:Food Microbiology; D006801:Humans; D007777:Lactobacillaceae / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D008087:Listeria / Q000187:drug effects / Q000254:growth & development; D008892:Milk / Q000382:microbiology*; D019936:Probiotics / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D001430:Bacteriocins