title: [The effect of polymorphism F279Y of GHR gene on milk production trait in Chinese Holstein cattle].
abstract: OBJECTIVE
  To study the effect of the polymorphism F279Y of the growth hormone receptor (GHR) gene on milk yield and composition in Chinese Holstein cattle.
  
  
  METHODS
  Hundred thirty two Chinese Holstein cattle were selected as study materials, according to DHI production performance method to get the data of milk yield and composition; PCR- SSCP and sequencing method were used to detect the genotypes; least square method was used to acquire correlation analysis.
  
  
  RESULTS
  Chinese Holstein cattle F279Y of GHR gene loci A and T allele frequency were 0.68 and 0.32, respectively, the experimental group significantly deviated from Hardy Weinberg equilibrium (P < 0.01); 305 d milk yield of AA genotype was significantly higher than AT type (P < 0.05), 305 d milk fat yield, 305 d milk protein yield and 305 d lactose of AT type had better trend than those of AA type in numeric; Therefore, allele A was dominant gene of high milk yield, allele T has positive effect on milk composition.
  
  
  CONCLUSIONS
  Mutation F279Y of GHR gene can be used as genetic markers in Chinese Holstein milk production traits of marker assisted selection (MAS) breeding.
authors.affiliation: Department of Chemistry and Life Sciences, Gansu Normal University for Nationalities, Hezuo 747000, China.
authors.forename: Yan-Nan
authors.initials: YN
authors.lastname: Ma
authors.affiliation: Department of Chemistry and Life Sciences, Gansu Normal University for Nationalities, Hezuo 747000, China.
authors.forename: Peng-Jia
authors.initials: PJ
authors.lastname: He
authors.affiliation: Department of Chemistry and Life Sciences, Gansu Normal University for Nationalities, Hezuo 747000, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Animal Science and Technology, College of Gansu Agricultural University, Lanzhou 730070, China.
authors.forename: Zhao-Min
authors.initials: ZM
authors.lastname: Lei
authors.affiliation: Animal Science and Technology, College of Gansu Agricultural University, Lanzhou 730070, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Animal Science and Technology, College of Gansu Agricultural University, Lanzhou 730070, China.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Wu
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D005260:Female; D005838:Genotype; D007774:Lactation; D008892:Milk / Q000378:metabolism*; D017354:Point Mutation; D011986:Receptors, Somatotropin / Q000235:genetics*
chemical_list: D011986:Receptors, Somatotropin