title: The aberrant expression and localization of prohibitin during apoptosis of human cholangiocarcinoma Mz-ChA-1 cells.
abstract: In this study, we aimed to investigate the aberrant expression and shift in localization of prohibitin (PHB) during apoptosis of human cholangiocarcinoma cells. Our study demonstrated that PHB was expressed primarily in the cytoplasm and only a little in the nucleus. However, PHB expression significantly decreased, and its localization shifted from the cytoplasm to the nucleus during apoptosis. PHB co-localized with AIF, Rb, p53, and c-Fos, but the region of co-localization was altered after treatment. Meanwhile, we detected a direct interaction between PHB and both p53 and Rb in Mz-ChA-1 cells. These results suggest that the altered localization and expression of PHB, as well as its co-localization with related oncogenes and tumor suppressor genes, can affect the apoptosis of Mz-ChA-1 cells.
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen 361005, China; School of Life Sciences and Engineering, Henan University of Urban Construction, Pingdingshan 467044, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen 361005, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen 361005, China.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Li
authors.affiliation: Center Laboratory, The First Affiliated Hospital of Xiamen University, Xiamen 361003, China. Electronic address: shendongyan@163.com.
authors.forename: Dong-Yan
authors.initials: DY
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen 361005, China. Electronic address: chenqx@xmu.edu.cn.
authors.forename: Qing-Xi
authors.initials: QX
authors.lastname: Chen
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D051033:Apoptosis Inducing Factor / Q000378:metabolism / Q000648:ultrastructure; D063646:Carcinogenesis; D045744:Cell Line, Tumor; D018281:Cholangiocarcinoma / Q000378:metabolism* / Q000473:pathology; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D015972:Gene Expression Regulation, Neoplastic; D016762:Genes, fos / Q000235:genetics; D006801:Humans; D000091402:Prohibitins; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics; D016160:Retinoblastoma Protein / Q000378:metabolism / Q000648:ultrastructure; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism / Q000648:ultrastructure
chemical_list: C494829:AIFM1 protein, human; D051033:Apoptosis Inducing Factor; C000717328:PHB protein, human; D000091402:Prohibitins; D012097:Repressor Proteins; D016160:Retinoblastoma Protein; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53
keywords: Human cholangiocarcinoma; Interaction; Localization shift; Prohibitin