title: Lobeline shows protective effects against MPTP-induced dopaminergic neuron death and attenuates behavior deficits in animals.
abstract: We previously demonstrated that lobeline effectively inhibited dopamine transporter (DAT)-mediated dopamine (DA) transportation. Therefore, the present study aimed to investigate whether lobeline shows protective effects against neurotoxin-induced cell deathin vivo. Mice were administered 30 mg/kg 1-methyl-4-phenyl-1,2,3,6-tetra-hydropyridine (MPTP) and treated with 80 mg/kg L-dopa, 10 mg/kg GBR12935 or 1 or 3 mg/kg lobeline, respectively, via injection. Rotarod and swim tests as well as tyrosine hydroxylase (TH) immunohistochemistry were carried out to evaluate the effects of these drugs. Compared with L-DA and GBR12935, lobeline (3 mg/kg administered via intraperitoneal injection) on behavior and dopaminergic neurons. Compared with L-DA and GBR12935, lobeline (3 mg/kg injected subcutaneously) significantly reduced MPTP induced locomotive deficits detected in behavioral tests. In addition, TH immunostaining showed that lobeline (3 mg/kg) markedly decreased the neurotoxin-induced immunoreactivity loss in the substantia nigra and striatum. Lobeline may be useful in the protection of dopaminergic neurons and may alleviate the symptoms of Parkinson's disease.
authors.affiliation: Department of Neurosurgery, Henan Province People's Hospital, Zhengzhou, Henan 450003, P.R. China.
authors.forename: Chao-Yue
authors.initials: CY
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Henan Province People's Hospital, Zhengzhou, Henan 450003, P.R. China.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, Henan Province People's Hospital, Zhengzhou, Henan 450003, P.R. China.
authors.forename: Xi-Wen
authors.initials: XW
authors.lastname: Shi
authors.affiliation: Department of Neurosurgery, Henan Province People's Hospital, Zhengzhou, Henan 450003, P.R. China.
authors.forename: Jia-Dong
authors.initials: JD
authors.lastname: Zhang
keywords: 1-methyl-4-phenyl-1,2,3,6-tetra-hydropyridine; Parkinson’s disease; dopamine transporter; lobeline; substantia nigra