title: RNA-Seq derived identification of differential transcription in the chrysanthemum leaf following inoculation with Alternaria tenuissima.
abstract: BACKGROUND
  A major production constraint on the important ornamental species chrysanthemum is black spot which is caused by the necrotrophic fungus Alternaria tenuissima. The molecular basis of host resistance to A. tenuissima has not been studied as yet in any detail. Here, high throughput sequencing was taken to characterize the transcriptomic response of the chrysanthemum leaf to A. tenuissima inoculation.
  
  
  RESULTS
  The transcriptomic data was acquired using RNA-Seq technology, based on the Illumina HiSeq™ 2000 platform. Four different libraries derived from two sets of leaves harvested from either inoculated or mock-inoculated plants were characterized. Over seven million clean reads were generated from each library, each corresponding to a coverage of >350,000 nt. About 70% of the reads could be mapped to a set of chrysanthemum unigenes. Read frequency was used as a measure of transcript abundance and therefore as an identifier of differential transcription in the four libraries. The differentially transcribed genes identified were involved in photosynthesis, pathogen recognition, reactive oxygen species generation, cell wall modification and phytohormone signalling; in addition, a number of varied transcription factors were identified. A selection of 23 of the genes was transcription-profiled using quantitative RT-PCR to validate the RNA-Seq output.
  
  
  CONCLUSIONS
  A substantial body of chrysanthemum transcriptomic sequence was generated, which led to a number of insights into the molecular basis of the host response to A. tenuissima infection. Although most of the differentially transcribed genes were up-regulated by the presence of the pathogen, those involved in photosynthesis were down-regulated.
authors.forename: Huiyun
authors.initials: H
authors.lastname: Li
authors.forename: Sumei
authors.initials: S
authors.lastname: Chen
authors.forename: Aiping
authors.initials: A
authors.lastname: Song
authors.forename: Haibin
authors.initials: H
authors.lastname: Wang
authors.forename: Weimin
authors.initials: W
authors.lastname: Fang
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Guan
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. jiangjiafu@njau.edu.cn.
authors.forename: Jiafu
authors.initials: J
authors.lastname: Jiang
authors.forename: Fadi
authors.initials: F
authors.lastname: Chen
mesh_terms: D000528:Alternaria / Q000502:physiology*; D002874:Chromosome Mapping; D031202:Chrysanthemum / Q000235:genetics* / Q000382:microbiology*; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics*; D054884:Host-Pathogen Interactions / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D017423:Sequence Analysis, RNA; D015398:Signal Transduction / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors