title: Bcl-2-associated transcription factor 1 interacts with fragile X-related protein 1.
abstract: The absence of fragile X mental retardation protein (FMRP) causes fragile X syndrome (FXS), which is the leading cause of hereditary mental retardation. Fragile X-related protein 1 (FXR1P), which plays an important role in normal muscle development, is one of the two autosomal paralogs of FMRP. To understand the functions of FXR1P, we screened FXR1P-interacting proteins by using a yeast two-hybrid system. The fragile X-related gene 1 (FXR1) was fused to pGBKT7 and then used as the bait to screen the human fetal brain cDNA library. The screening results revealed 10 FXR1P-interacting proteins including Bcl-2-associated transcription factor 1 (BTF). The interaction between FXR1P and BTF was confirmed by using both β-galactosidase assay and growth test in selective media. Co-immunoprecipitation assay in mammalian cells was also carried out to confirm the FXR1P/BTF interaction. Moreover, we confirmed that BTF co-localized with FXR1P in the cytoplasm around the nucleus in rat vascular smooth muscle cells by using confocal fluorescence microscopy. These results provide clues to elucidate the relationship between FXR1P and FXS.
authors.affiliation: Department of Biochemistry and Biology, South China University, Hengyang 421001, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ma
authors.forename: Changbo
authors.initials: C
authors.lastname: Wang
authors.forename: Binyuan
authors.initials: B
authors.lastname: Li
authors.forename: Lingxue
authors.initials: L
authors.lastname: Qin
authors.forename: Jiao
authors.initials: J
authors.lastname: Su
authors.forename: Manjun
authors.initials: M
authors.lastname: Yang
authors.forename: Shuya
authors.initials: S
authors.lastname: He
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D060066:Protein Interaction Maps; D016601:RNA-Binding Proteins / Q000378:metabolism*; D051381:Rats; D012097:Repressor Proteins / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: C119386:BCLAF1 protein, human; C096916:FXR1 protein, human; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; D025521:Tumor Suppressor Proteins
keywords: Bcl-2-associated transcription factor 1; fragile X-related protein 1; protein interaction