title: Subfatin is a novel adipokine and unlike Meteorin in adipose and brain expression.
abstract: OBJECTIVE
  Adipose tissue releases adipokines that play important roles in metabolic and cardio-cerebro-vascular homeostasis. This study was to discover novel adipokines using caloric restriction model.
  
  
  METHODS
  Adipokine candidates were captured by gene array and bioinformatics analysis and verified by preparation of recombinant protein and antibody.
  
  
  RESULTS
  We established a potential secreted protein database containing 208 genes and identified a novel adipokine, Subfatin, that was the highest expressed in subcutaneous fat of both rodents and humans among 15 detected tissues. The secreted mammalian Subfatin was a glycosylated protein. Subfatin was located diffusely throughout the adipose tissue except lipid droplets, with comparable expression between adipocytes and stromal cells, but much lower expression in macrophages than adipocytes. Subfatin was downregulated in white adipose tissue of caloric restriction rats, whereas dramatically upregulated during white adipocyte differentiation as well as in white adipose tissue of diet-induced obese mice. Subfatin was annotated as Meteorin-like (Metrnl) in public databases, a similar transcript of Meteorin (Metrn, also known as glial cell differentiation regulator). Meteorin displayed a brain-specific expression and was scarce in various adipose tissues, in contrast to the tissue expression patterns of Subfatin.
  
  
  CONCLUSIONS
  Subfatin is a novel adipokine regulated by adipogenesis and obesity, with tissue distribution different from its homologue Meteorin.
authors.affiliation: Department of Pharmacology, Second Military Medical University, Shanghai, China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Li
authors.forename: Si-Li
authors.initials: SL
authors.lastname: Zheng
authors.forename: Pei
authors.initials: P
authors.lastname: Wang
authors.forename: Tian-Ying
authors.initials: TY
authors.lastname: Xu
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Guan
authors.forename: Yi-Jie
authors.initials: YJ
authors.lastname: Zhang
authors.forename: Chao-Yu
authors.initials: CY
authors.lastname: Miao
mesh_terms: D050156:Adipogenesis / Q000502:physiology; D054392:Adipokines / Q000235:genetics / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism*; D052436:Adipose Tissue, White / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism*; D031204:Caloric Restriction; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008297:Male; D051379:Mice; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009765:Obesity / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D050151:Subcutaneous Fat / Q000378:metabolism
chemical_list: D054392:Adipokines; D036341:Intercellular Signaling Peptides and Proteins; C502097:METRN protein, human; C546969:Metrn protein, rat; C000593412:Metrnl protein, human; C000593411:Metrnl protein, rat; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; C570859:cometin protein, mouse; C502098:meteorin protein, mouse
keywords: Adipokines; Brain; Caloric restriction; Meteorin; Subfatin