title: Categorisation of sugar acid dehydratases in Aspergillus niger.
abstract: In the genome of Aspergillus niger five genes were identified coding for proteins with homologies to sugar acid dehydratases. The open reading frames were expressed in Saccharomyces cerevisiae and the activities tested with a library of sugar acids. Four genes were identified to code for proteins with activities with sugar acids: an l-galactonate dehydratase (gaaB), two d-galactonate dehydratases (dgdA, dgdB) and an l-rhamnonate dehydratase (lraC). The specificities of the proteins were characterised. The l-galactonate dehydratase had highest activity with l-fuconate, however it is unclear whether the enzyme is involved in l-fuconate catabolism. None of the proteins showed activity with galactaric acid or galactarolactone.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Cx. P. 19046 Centro Politécnico, Curitiba 81531-980, Paraná, Brazil; VTT Technical Research Centre of Finland, Tietotie 2, Espoo, P.O. Box 1500, 02044 VTT, Finland.
authors.forename: Francine A
authors.initials: FA
authors.lastname: Motter
authors.affiliation: VTT Technical Research Centre of Finland, Tietotie 2, Espoo, P.O. Box 1500, 02044 VTT, Finland.
authors.forename: Joosu
authors.initials: J
authors.lastname: Kuivanen
authors.affiliation: VTT Technical Research Centre of Finland, Tietotie 2, Espoo, P.O. Box 1500, 02044 VTT, Finland.
authors.forename: Hanna
authors.initials: H
authors.lastname: Keränen
authors.affiliation: VTT Technical Research Centre of Finland, Tietotie 2, Espoo, P.O. Box 1500, 02044 VTT, Finland.
authors.forename: Satu
authors.initials: S
authors.lastname: Hilditch
authors.affiliation: VTT Technical Research Centre of Finland, Tietotie 2, Espoo, P.O. Box 1500, 02044 VTT, Finland.
authors.forename: Merja
authors.initials: M
authors.lastname: Penttilä
authors.affiliation: VTT Technical Research Centre of Finland, Tietotie 2, Espoo, P.O. Box 1500, 02044 VTT, Finland. Electronic address: Peter.Richard@vtt.fi.
authors.forename: Peter
authors.initials: P
authors.lastname: Richard
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D005656:Fungal Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D005800:Genes, Fungal; D006836:Hydro-Lyases / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D016366:Open Reading Frames; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013400:Sugar Acids / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D013400:Sugar Acids; D006836:Hydro-Lyases; C019607:galactonate dehydratase; C002930:L-fuconate dehydratase
keywords: 2-Keto-3-deoxy acid; Dehydratase; d-Galactonate; l-Fuconate; l-Galactonate; l-Rhamnonate