title: A computation using mutually exclusive processing is sufficient to identify specific Hedgehog signaling components.
abstract: A system of more than one part can be deciphered by observing differences between the parts. A simple way to do this is by recording something absolute displaying a trait in one part and not in another: in other words, mutually exclusive computation. Conditional directed expression in vivo offers processing in more than one part of the system giving increased computation power for biological systems analysis. Here, I report the consideration of these aspects in the development of an in vivo screening assay that appears sufficient to identify components specific to a system.
authors.affiliation: Okinawa Institute of Science and Technology Graduate University Okinawa, Japan ; Department of Frontier Bioscience, Hosei University Koganei, Tokyo, Japan.
authors.forename: Spencer J
authors.initials: SJ
authors.lastname: Spratt
keywords: computation; genetics; methods; mutual exclusivity; specificity; systems analysis