title: Development of Pasteurella multocida-loaded microparticles for hemorrhagic septicemia vaccine.
abstract: In this study, Pasteurella multocida-loaded alginate microparticles (MPs) for subcutaneous vaccination was developed by emulsification-cross-linking technique. Formulation parameter was varied as a ratio of polymer and bacterin. Optical microscopy revealed spherical particles with uniformly distribution. A mean particle size of approximately 6 µm has been successfully constructed using simple mixer and ultrasonic probe. The zeta potential of the MPs showed negatively charge of approximately -23 mV determined by Zeta Pals® analyzer. The entrapment efficiency and the in vitro bacterin released profile could be controlled by varying the amount of alginate. The high entrapment efficiency up to 69% was achieved with low concentration of alginate. The MPs possessed a slow bacterin release profile, up to 30 days. In vivo safety and potency tests were proved that the alginate MPs were safe and induced protective immunity in mice. In addition, after storage for 6 months at either 4 °C or room temperature, the protective immunity in mice was maintained.
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Naresuan University , Phitsanulok , Thailand .
authors.forename: Pataranapa
authors.initials: P
authors.lastname: Nimtrakul
authors.forename: Ratchanee
authors.initials: R
authors.lastname: Atthi
authors.forename: Nanteetip
authors.initials: N
authors.lastname: Limpeanchob
authors.forename: Waree
authors.initials: W
authors.lastname: Tiyaboonchai
mesh_terms: D000464:Alginates / Q000008:administration & dosage* / Q000138:chemical synthesis; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000138:chemical synthesis; D004337:Drug Carriers / Q000008:administration & dosage / Q000138:chemical synthesis; D020723:Glucuronic Acid / Q000008:administration & dosage / Q000138:chemical synthesis; D006483:Hemorrhagic Septicemia / Q000473:pathology / Q000517:prevention & control*; D006603:Hexuronic Acids / Q000008:administration & dosage / Q000138:chemical synthesis; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008863:Microspheres; D016979:Pasteurella multocida
chemical_list: D000464:Alginates; D001428:Bacterial Vaccines; D004337:Drug Carriers; D006603:Hexuronic Acids; D020723:Glucuronic Acid
keywords: Alginate; Pasteurella multocida; hemorrhagic septicemia; microparticles; vaccine