title: Seasonal dynamics and diversity of bacteria in retail oyster tissues.
abstract: Oysters are one of the important vehicles for the transfer of foodborne pathogens. It was reported that bacteria could be bio-accumulated mainly in the gills and digestive glands. In artificially treated oysters, bacterial communities have been investigated by culture-independent methods after harvest. However, little information is available on the seasonal dynamics of bacterial accumulation in retail oyster tissues. In this study, retail oysters were collected from local market in different seasons. The seasonal dynamics and diversity of bacteria in oyster tissues, including the gills, digestive glands and residual tissues, were analyzed by denaturing gradient gel electrophoresis (DGGE). It was interesting that the highest bacterial diversity appeared in the Fall season, not in summer. Our results indicated that Proteobacteria was the predominant member (23/46) in oyster tissues. Our results also suggested that bacterial diversity in gills was higher than that in digestive glands and other tissues. In addition, not all the bacteria collected from surrounding water by gills were transferred to digestive glands. On the other hand, few bacteria were found in oyster tissues except in the gills. Therefore, the gills could be the best candidate target tissue for monitoring of pathogenic bacteria either to human or to oyster.
authors.affiliation: MOST-USDA Joint Research Center for Food Safety & Bor Luh Food Safety Center, School of Agriculture and Biology & State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, PR China. Electronic address: dapengwang@163.com.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Wang
authors.affiliation: MOST-USDA Joint Research Center for Food Safety & Bor Luh Food Safety Center, School of Agriculture and Biology & State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: MOST-USDA Joint Research Center for Food Safety & Bor Luh Food Safety Center, School of Agriculture and Biology & State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cui
authors.affiliation: MOST-USDA Joint Research Center for Food Safety & Bor Luh Food Safety Center, School of Agriculture and Biology & State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Xianming
authors.initials: X
authors.lastname: Shi
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics; D018407:Bacterial Physiological Phenomena; D044822:Biodiversity; D005516:Food Microbiology; D005880:Gills / Q000382:microbiology; D010125:Ostreidae / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012621:Seasons
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Bacterial diversity; DGGE; Retail oyster; Seasonal dynamics; Tissues