title: Determination of xanthatin by ultra high performance liquid chromatography coupled with triple quadrupole mass spectrometry: application to pharmacokinetic study of xanthatin in rat plasma.
abstract: A sensitive, specific and rapid ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method has been established to study pharmacokinetic properties of xanthatin. Xanthatin, a compound which belongs to sesquiterpene lactone group, was determined in rat plasma with psoralen as internal standard. Chromatographic separation was performed on an Agilent Zorbax Eclipse plus C18 column (50 mm × 2.1 mm, 3.5 μm) with gradient elution system at a flow rate of 0.3 mL/min. The mobile phase was composed of methanol and 0.1% formic acid water solution. Analysis was performed under a triple-quadruple tandem mass-spectrometer with an electrospray ionization (ESI) source via the multiple reaction monitoring (MRM) mode to determine xanthatin at [M+H](+)m/z 247.3→m/z 205.2 and that of IS at [M+H](+)m/z 187.1→m/z 143.0 within 5 min. The assay method exhibited good separation of xanthatin from the interference of endogenous substances. The lower limit of quantification (LLOQ) was 1 ng/mL, with a good linearity within the concentration range of 1-5000 ng/mL (r=0.9990). Intra-day and inter-day precision RSD was less than 9.27%; intra-day and inter-day accuracy was 88.48% and 102.25% respectively. The extraction recoveries of xanthatin range from 82.12% to 89.55%, and the extraction RSD was less than 9.01%. The established LC-ESI-MS/MS method is rapid and sensitive, which has been successfully applied to quantify xanthatin in rat plasma for the first time.
authors.affiliation: Nanjing University of Chinese Medicine, Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing 210023, China; Pharmacy College of Nanjing University of TCM, Nanjing 210023, China.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Yan
authors.affiliation: Nanjing University of Chinese Medicine, Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing 210023, China; Pharmacy College of Nanjing University of TCM, Nanjing 210023, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Li
authors.affiliation: Nantong Hospital of Traditional Chinese Medicine, Nantong 226001, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Shanghai Dahua Hospital of Shanghai Xuhui District, Shanghai 200031, China.
authors.forename: Donghao
authors.initials: D
authors.lastname: Xie
authors.affiliation: Nanjing University of Chinese Medicine, Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing 210023, China; Pharmacy College of Nanjing University of TCM, Nanjing 210023, China.
authors.forename: Zebin
authors.initials: Z
authors.lastname: Weng
authors.affiliation: Nanjing University of Chinese Medicine, Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing 210023, China; Pharmacy College of Nanjing University of TCM, Nanjing 210023, China.
authors.forename: Baochang
authors.initials: B
authors.lastname: Cai
authors.affiliation: Nanjing University of Chinese Medicine, Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing 210023, China; Pharmacy College of Nanjing University of TCM, Nanjing 210023, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.affiliation: Nanjing University of Chinese Medicine, Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing 210023, China; Pharmacy College of Nanjing University of TCM, Nanjing 210023, China. Electronic address: liweidong0801@163.com.
authors.forename: Weidong
authors.initials: W
authors.lastname: Li
authors.affiliation: Nanjing University of Chinese Medicine, Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing 210023, China; Pharmacy College of Nanjing University of TCM, Nanjing 210023, China. Electronic address: cpuczp2000@hotmail.com.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000493:pharmacokinetics*; D005663:Furans / Q000097:blood / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*; D031210:Xanthium / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D005663:Furans; C022186:xanthatin
keywords: Pharmacokinetics; Ultra high performance liquid chromatography tandem mass spectrometry (UHPLC–ESI-MS/MS); Xanthatin