title: Methyl-accepting chemotaxis proteins 3 and 4 are responsible for Campylobacter jejuni chemotaxis and jejuna colonization in mice in response to sodium deoxycholate.
abstract: Methyl-accepting chemotaxis proteins (MCPs), also termed transducer-like proteins (Tlps), serve as sensors in bacterial chemotactic signalling, and detect attractants and promote bacterial movement towards suitable sites for colonization. Campylobacter jejuni is a leading cause of human enteritis, but the mechanisms responsible for bacterial chemotaxis and early colonization in the jejunum of hosts are poorly understood. In the present study, we identified several types of bile and sodium deoxycholate (SDC) acting as chemotactic attractants of C. jejuni strain NCTC 11168-O in vitro, in which SDC was the most efficient chemoattractant. In mice with bile duct ligation, the wild-type strain displayed a markedly attenuated ability for colonization. Blockage of Tlp3 or Tlp4 protein with antibody or disruption of the tlp3 or tlp4 gene (Δtlp3 or Δtlp4) caused a significant inhibition of SDC-induced chemotaxis and attenuation for colonization on jejunal mucosa in mice of the bacterium. Disruption of both the genes (Δtlp3/Δtlp4) resulted in the absence of bacterial chemotaxis and colonization, while the tlp-gene-complemented mutants (CΔtlp3 and CΔtlp4) reacquired these abilities. The results indicate that SDC is an effective chemoattractant for C. jejuni, and Tlp3 and Tlp4 are the SDC-specific sensor proteins responsible for the bacterial chemoattraction.
authors.affiliation: Department of Acute Infectious Diseases Control and Prevention, Jiangsu Provincial Center for Disease Control and Prevention, Jiangsu Nanjing 210009, PR China.|Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, PR China.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Li
authors.affiliation: Medical School of Jinhua Occupational Technique College, Jinhua, Zhejiang 321007, PR China.
authors.forename: Hongqiang
authors.initials: H
authors.lastname: Lou
authors.affiliation: Molecular Cell Biology and Health Sciences Research Institute, University of California, Merced, CA 95343, USA.
authors.forename: David M
authors.initials: DM
authors.lastname: Ojcius
authors.affiliation: Faculty of Basic Medicine, Zhejiang Medical College, Hangzhou, Zhejiang 310053, PR China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Sun
authors.affiliation: Department of Neurology and Neuroscience, New York Presbyterian Hospital and Hospital For Special Surgery, Cornell University Weill Medical College, NY 10021, USA.
authors.forename: Dexter
authors.initials: D
authors.lastname: Sun
authors.affiliation: Depatment of Clinical Laboratory, Zhejiang Provincial Hospital of Traditional Chinese Medicine, Hangzhou, Zhejiang, PR China.
authors.forename: Jinfang
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, PR China.
authors.forename: Xu'ai
authors.initials: X
authors.lastname: Lin
authors.affiliation: Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, PR China.|Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yan
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D001646:Bile; D002169:Campylobacter Infections / Q000382:microbiology*; D016123:Campylobacter jejuni / Q000187:drug effects / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D002630:Chemotactic Factors / Q000378:metabolism*; D002633:Chemotaxis; D003840:Deoxycholic Acid / Q000378:metabolism*; D005260:Female; D055786:Gene Knockout Techniques; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D000072236:Methyl-Accepting Chemotaxis Proteins; D008745:Methylation; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011817:Rabbits; D011994:Recombinant Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D002630:Chemotactic Factors; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins; D011994:Recombinant Proteins; D003840:Deoxycholic Acid