title: Near-infrared-fluorescence imaging of lymph nodes by using liposomally formulated indocyanine green derivatives.
abstract: Liposomally formulated indocyanine green (LP-ICG) has drawn much attention as a highly sensitive near-infrared (NIR)-fluorescence probe for tumors or lymph nodes in vivo. We synthesized ICG derivatives tagged with alkyl chains (ICG-Cn), and we examined NIR-fluorescence imaging for lymph nodes in the lower extremities of mice by using liposomally formulated ICG-Cn (LP-ICG-Cn) as well as conventional liposomally formulated ICG (LP-ICG) and ICG. Analysis with a noninvasive preclinical NIR-fluorescence imaging system revealed that LP-ICG-Cn accumulates in only the popliteal lymph node 1h after injection into the footpad, whereas LP-ICG and ICG accumulate in the popliteal lymph node and other organs like the liver. This result indicates that LP-ICG-Cn is a useful NIR-fluorescence probe for noninvasive in vivo bioimaging, especially for the sentinel lymph node.
authors.affiliation: Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan; Department of Bioinformatics, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan; Department of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo 153-8902, Japan.
authors.forename: Taro
authors.initials: T
authors.lastname: Toyota
authors.affiliation: Department of Medical System Engineering, Faculty of Engineering, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Fujito
authors.affiliation: Department of Bioinformatics, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Suganami
authors.affiliation: Division of Nanoscience, Graduate School of Advanced Integration Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Ouchi
authors.affiliation: Division of Nanoscience, Graduate School of Advanced Integration Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Ooishi
authors.affiliation: Yamada Chemical Co. Ltd, 1-1 Kamichoshi-cho, Kamitoba, Minami-ku, Kyoto 601-8105, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Aoki
authors.affiliation: Yamada Chemical Co. Ltd, 1-1 Kamichoshi-cho, Kamitoba, Minami-ku, Kyoto 601-8105, Japan.
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Onoue
authors.affiliation: Yamada Chemical Co. Ltd, 1-1 Kamichoshi-cho, Kamitoba, Minami-ku, Kyoto 601-8105, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Muraki
authors.affiliation: Yamada Chemical Co. Ltd, 1-1 Kamichoshi-cho, Kamitoba, Minami-ku, Kyoto 601-8105, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Madono
authors.affiliation: Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Fujinami
authors.affiliation: Department of Bioinformatics, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tamura
authors.affiliation: Center for Frontier Medical Engineering, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan; Department of Frontier Surgery, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan. Electronic address: hhayashi@faculty.chiba-u.jp.
authors.forename: Hideki
authors.initials: H
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D003952:Diagnostic Imaging; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D007208:Indocyanine Green / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D008198:Lymph Nodes / Q000473:pathology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D015394:Molecular Structure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013050:Spectrometry, Fluorescence; D019265:Spectroscopy, Near-Infrared
chemical_list: D005456:Fluorescent Dyes; D008081:Liposomes; D007208:Indocyanine Green
keywords: Indocyanine green (ICG); Liposome; Lymph node; Near-infrared (NIR)-fluorescence probe; Phospholipid