title: Shake and stew: a non-destructive PCR-ready DNA isolation method from a single preserved fish larva.
abstract: A rapid non-destructive alternative to isolate DNA from an individual fish larva is presented, based on the suspension of epithelial cells through vortex forces, and the release of DNA in a heated alkaline solution. DNA from >6056 fish larvae isolated using this protocol has yielded a high PCR amplification success rate (>93%), suggesting its applicability to other taxonomic groups or sources when tissue amount is the limiting factor.
authors.affiliation: Department of Marine Biology, Texas A&M University at Galveston, OCSB 3029, Galveston, TX, 77554, U.S.A.; Department of Wildlife and Fisheries Sciences, Texas A&M University, 210 Nagle Hall, TAMU 2258, College Station, TX, 75044, U.S.A.
authors.forename: J R
authors.initials: JR
authors.lastname: Alvarado Bremer
authors.forename: B L
authors.initials: BL
authors.lastname: Smith
authors.forename: D L
authors.initials: DL
authors.lastname: Moulton
authors.forename: C-P
authors.initials: CP
authors.lastname: Lu
authors.forename: M
authors.initials: M
authors.lastname: Cornic
mesh_terms: D000818:Animals; D004247:DNA / Q000302:isolation & purification*; D004847:Epithelial Cells; D005399:Fishes; D007814:Larva / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D013048:Specimen Handling / Q000379:methods
chemical_list: D004247:DNA
keywords: Istiophorus platypterus; NaOH; Thunnus albacares; genetics; ichthyoplankton; minimally invasive