title: Multinuclear nanoliter one-dimensional and two-dimensional NMR spectroscopy with a single non-resonant microcoil.
abstract: Nuclear magnetic resonance (NMR) spectroscopy is a powerful analytical technique, but its low sensitivity and highly sophisticated, costly, equipment severely constrain more widespread applications. Here we show that a non-resonant planar transceiver microcoil integrated in a microfluidic chip (detection volume 25 nl) can detect different nuclides in the full broad-band range of Larmor frequencies (at 9.4 T from 61 to 400 MHz). Routine one-dimensional (1D) and two-dimensional (2D), homo- and heteronuclear experiments can be carried out using the broad-band coil set-up. Noteworthy, heteronuclear 2D experiments can be performed in a straightforward manner on virtually any combination of nuclides (from classical ¹H-¹³C to more exotic combinations like ¹⁹F-³¹P) both in coupled and decoupled mode. Importantly, the concept of a non-resonant system provides magnetic field-independent NMR probes; moreover, the small-volume alleviates problems related to field inhomogeneity, making the broad-band coil an attractive option for, for example, portable and table-top NMR systems.
authors.affiliation: Laboratory of SupraMolecular Chemistry & Technology, University of Twente, PO BOX 217, 7500 AE Enschede, The Netherlands.
authors.forename: Raluca M
authors.initials: RM
authors.lastname: Fratila
authors.affiliation: 1] Instituto Regional de Investigación Científica Aplicada (IRICA), Universidad de Castilla-La Mancha, Avda. Camilo José Cela, s/n, 13071 Ciudad Real, Spain [2] Fundación Parque Científico y Tecnológico de Albacete, Paseo de la Innovación, 1, 02006 Albacete, Spain.
authors.forename: M Victoria
authors.initials: MV
authors.lastname: Gomez
authors.affiliation: Extra Byte, Via Raffaello Sanzio 22C, I-20022 Castano Primo, Italy.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Sýkora
authors.affiliation: 1] Laboratory of SupraMolecular Chemistry & Technology, University of Twente, PO BOX 217, 7500 AE Enschede, The Netherlands [2] Instituto Regional de Investigación Científica Aplicada (IRICA), Universidad de Castilla-La Mancha, Avda. Camilo José Cela, s/n, 13071 Ciudad Real, Spain [3] Laboratory of BioNanoTechnology, Wageningen University, PO BOX 8038, 6700 EK Wageningen, The Netherlands.
authors.forename: Aldrik H
authors.initials: AH
authors.lastname: Velders