title: Functional analysis of novel Rab GTPases identified in the proteome of purified Legionella-containing vacuoles from macrophages.
abstract: The opportunistic pathogen Legionella pneumophila employs the Icm/Dot type IV secretion system and ∼300 different effector proteins to replicate in macrophages and amoebae in a distinct 'Legionella-containing vacuole' (LCV). LCVs from infected RAW 264.7 macrophages were enriched by immuno-affinity separation and density gradient centrifugation, using an antibody against the L. pneumophila effector SidC, which specifically binds to the phosphoinositide PtdIns(4)P on the pathogen vacuole membrane. The proteome of purified LCVs was determined by mass spectro-metry (data are available via ProteomeXchange with identifier PXD000647). The proteomics analysis revealed more than 1150 host proteins, including 13 small GTPases of the Rab family. Using fluorescence microscopy, 6 novel Rab proteins were confirmed to localize on pathogen vacuoles harbouring wild-type but not ΔicmT mutant L. pneumophila. Individual depletion of 20 GTPases by RNA interference indicated that endocytic GTPases (Rab5a, Rab14 and Rab21) restrict intracellular growth of L. pneumophila, whereas secretory GTPases (Rab8a, Rab10 and Rab32) implicated in Golgi-endosome trafficking promote bacterial replication. Upon silencing of Rab21 or Rab32, fewer LCVs stained positive for Rab4 or Rab9, implicated in secretory or retrograde trafficking respectively. Moreover, depletion of Rab8a, Rab14 or Rab21 significantly decreased the number of SidC-positive LCVs, suggesting that PtdIns(4)P is reduced under these conditions. L. pneumophila proteins identified in purified LCVs included proteins putatively implicated in phosphorus metabolism and as many as 60 Icm/Dot-translocated effectors, which are likely required early during infection. Taken together, the phagocyte and Legionella proteomes of purified LCVs lay the foundation for further hypothesis-driven investigations of the complex process of pathogen vacuole formation.
authors.affiliation: Department of Medicine, Max von Pettenkofer Institute, Ludwig-Maximilians University Munich, Munich, 80336, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Hoffmann
authors.forename: Ivo
authors.initials: I
authors.lastname: Finsel
authors.forename: Andreas
authors.initials: A
authors.lastname: Otto
authors.forename: Gudrun
authors.initials: G
authors.lastname: Pfaffinger
authors.forename: Eva
authors.initials: E
authors.lastname: Rothmeier
authors.forename: Michael
authors.initials: M
authors.lastname: Hecker
authors.forename: Dörte
authors.initials: D
authors.lastname: Becher
authors.forename: Hubert
authors.initials: H
authors.lastname: Hilbi
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D004023:Dictyostelium / Q000502:physiology; D054884:Host-Pathogen Interactions; D006801:Humans; D016952:Legionella pneumophila / Q000502:physiology*; D008264:Macrophages / Q000201:enzymology / Q000382:microbiology; D051379:Mice; D020543:Proteome / Q000378:metabolism*; D014617:Vacuoles / Q000201:enzymology* / Q000382:microbiology; D020691:rab GTP-Binding Proteins / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; C534216:SidC protein, Legionella pneumophila; D020691:rab GTP-Binding Proteins