title: miR-101 sensitizes A549 NSCLC cell line to CDDP by activating caspase 3-dependent apoptosis.
abstract: MicroRNA-101 (miR-101) is evidently downregulated in several types of cancer, including non-small cell lung cancer (NSCLC), and is crucial in sensitizing cells to chemotherapy drugs. The aim of the present study was to investigate the correlation between miR-101 and chemosensitivity in the A549 NSCLC cell line. Here, we used the human A549 cell line for transfection with an miR-101 overexpressing vector and detected the cytotoxic acticity, proliferation and apoptosis ofcis-diaminedichloroplatinum (CDDP) in A549-miR-101 and A549-mock cells. We demonstrated that overexpression of miR-101 sensitized A549 cells to CDDP, one of the most frequently used agents in curing or controlling NSCLC and enhanced CDDP-induced cell death and caspase 3-dependent apoptosis. In addition, miR-101 facilitated the inhibitory role of CDDP in A549 cell colony formation. Overall, the results of the present study demonstrated that miR-101 sensitizes the A549 NSCLC cell line to CDDP via the activation of caspase 3-dependent apoptosis.
authors.affiliation: Department of General Practice, Shandong University Hospital, Shandong University, Jinan, Shandong 250061, P.R. China.
authors.forename: Jiqing
authors.initials: J
authors.lastname: Yin
authors.affiliation: Department of Stomatology, Subsidiary Central Hospital, Shandong University, Jinan, Shandong 250061, P.R. China.
authors.forename: Mingguo
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of General Practice, Shandong University Hospital, Shandong University, Jinan, Shandong 250061, P.R. China.
authors.forename: Cuixiang
authors.initials: C
authors.lastname: Jin
authors.affiliation: Department of Stomatology, Stomatology Hospital of Shandong University, Jinan, Shandong 250061, P.R. China.
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Qi
keywords: A549; CDDP; NSCLC; chemosensitivity; miR-101; miRNA