title: Characterization of the binding of chrysoidine, an illegal food additive to bovine serum albumin.
abstract: Chrysoidine is an industrial azo dye and the presence of chrysoidine in water and food has become an environmental concern due to its negative effects on human beings. Binding of dyes to serum albumins significantly influence their absorption, distribution, metabolism, and excretion properties. In this work, the interactions of chrysoidine with bovine serum albumin (BSA) were explored. Isothermal titration calorimetry results reveal the binding stoichiometry of chrysoidine to BSA is 1:15.5, and van der Waals and hydrogen bonding interactions are the major driving force in the binding of chrysoidine to BSA. Molecular docking simulations show that chrysoidine binds to BSA at a cavity close to Sudlow site I in domain IIA. However, no detectable conformational change of BSA occurs in the presence of chrysoidine as revealed by UV-vis absorption, circular dichroism and fluorescence spectroscopy studies.
authors.affiliation: School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, Shandong Province, 27# Shanda Nanlu, Jinan 250100, PR China.
authors.forename: Bingjun
authors.initials: B
authors.lastname: Yang
authors.affiliation: School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, Shandong Province, 27# Shanda Nanlu, Jinan 250100, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Hao
authors.affiliation: School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, Shandong Province, 27# Shanda Nanlu, Jinan 250100, PR China.
authors.forename: Jiarong
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, Shandong Province, 27# Shanda Nanlu, Jinan 250100, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wei
authors.affiliation: School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, Shandong Province, 27# Shanda Nanlu, Jinan 250100, PR China.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, Shandong Province, 27# Shanda Nanlu, Jinan 250100, PR China. Electronic address: rutaoliu@sdu.edu.cn.
authors.forename: Rutao
authors.initials: R
authors.lastname: Liu
mesh_terms: D002942:Circular Dichroism; D005503:Food Additives / Q000378:metabolism*; D062105:Molecular Docking Simulation; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D010128:p-Aminoazobenzene / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D005503:Food Additives; C005562:chrysoidine; D012710:Serum Albumin, Bovine; D010128:p-Aminoazobenzene
keywords: Dyes; Isothermal titration calorimetry; Molecular docking; Proteins; Spectroscopy; Thermodynamics