title: [Identification and expression of cytochrome C in tumor-bearing mice model with neuroblastoma].
abstract: OBJECTIVE
  To screen, identify and verify the serum specific protein of neuroblastoma in a tumor-bearing murine model and speculate about the source of cytochrome C.
  
  
  METHODS
  NB cells (KP-N-NS) were inoculated subcutaneously into nude mice. And the sera samples of tumor-bearing mice (observation group, n = 14) and controls (control group, n = 25) were collected at 4 weeks to screen for and identify differentially expressed proteins. The method of surface-enhanced laser desorption-ionization time-of-flight mass spectrometry (SELDI-TOF-MS) was employed to screen the serum specific protein of neuroblastoma between two groups. Then serum protein targets were purified by high-performance liquid chromatography (HPLC) and identified by LC-MS/MS (LTQ).
  
  
  RESULTS
  By comparing protein peaks among two sera groups, we identified the peak (11 609) showing significant differential expression between two groups. The expression of peak (11 609) was 3338.4 ± 1410.9 in observation group and 59.8 ± 40.7 in control group. This peak was identified as murine cytochrome C.
  
  
  CONCLUSIONS
  Cytochrome C is a serum specific protein for neuroblastoma tumor and it comes from the apoptosis of non-tumor cells.
authors.affiliation: Department of Surgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Yan-li
authors.initials: YL
authors.lastname: Pang
authors.affiliation: Department of Surgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Da
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Surgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Surgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Surgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Qin
authors.affiliation: Department of Surgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Yu-xia
authors.initials: YX
authors.lastname: Fan
authors.affiliation: Department of Surgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Jun-jie
authors.initials: JJ
authors.lastname: Zhang
authors.affiliation: Department of Surgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China. Email: wjiaxiang@zzu.edu.cn.
authors.forename: Jia-xiang
authors.initials: JX
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000097:blood*; D045304:Cytochromes c / Q000097:blood*; D005260:Female; D051379:Mice; D008819:Mice, Nude; D009447:Neuroblastoma / Q000097:blood*; D010449:Peptide Mapping
chemical_list: D014408:Biomarkers, Tumor; D045304:Cytochromes c