title: The host specific NS3 glycosylation pattern reflects the virulence of Ibaraki virus in different hosts.
abstract: The non-structural protein NS3 was investigated in Ibaraki virus (IBAV), an epizootic hemorrhagic disease virus, serotype 2. Degree of NS3 glycosylation, cytopathic effect, and virus release efficiency were compared between mammalian and insect cells. The molecular weight of synthesized NS3 was compared in Western blot analysis following the removal of the glycochain by PNGase F treatment and revealed that glycosylation of NS3 occurred only in mammalian cells. Also, it was revealed that the amount of infectious IBAV in the extracellular fraction continued to increase for insect cells even after 60h post infection without disrupting cells. These results suggested that glycosylation of NS3 controls pathogenicity of IBAV in host cells to protect vector insects by altering the release pathway of assembled progeny viruses.
authors.affiliation: Laboratory of Veterinary Hygiene, Joint Faculty of Veterinary Medicine, Yamaguchi University, Japan.
authors.forename: Maho
authors.initials: M
authors.lastname: Urata
authors.affiliation: Laboratory of Veterinary Hygiene, Joint Faculty of Veterinary Medicine, Yamaguchi University, Japan. Electronic address: r.nabewo@gmail.com.
authors.forename: Rie
authors.initials: R
authors.lastname: Watanabe
authors.affiliation: Laboratory of Veterinary Hygiene, Joint Faculty of Veterinary Medicine, Yamaguchi University, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Iwata
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D015870:Gene Expression; D006031:Glycosylation; D018164:Hemorrhagic Disease Virus, Epizootic / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions; D006801:Humans; D008970:Molecular Weight; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014774:Virulence; D014779:Virus Replication
chemical_list: D017361:Viral Nonstructural Proteins
keywords: Host specific pathogenicity; NS3 glycosylation; Orbivirus; Virus release