title: Hybrid organic-inorganic monolithic enzymatic reactor with SBA-15 nanoparticles incorporated.
abstract: A novel enzymatic reactor was prepared by incorporating SBA-15 nanoparticles into hybrid organic-inorganic monolith and immobilizing trypsin with glutaraldehyde as bridging reagent. Preparation and operation conditions including nanoparticles percentage and residence time were optimized to improve the digestion efficiency. The digestion products were characterized by Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS) with sequence coverage of 50%, 93% and 71% for bovine serum albumin, myoglobin and cytochrome C, while consuming only about 19s in dynamic mode. Compared with enzymatic reactor without nanoparticles incorporated, the enzymatic reactor with SBA-15 nanoparticles embedded achieved higher digestion efficiency by introducing more trypsin, which was originated from combination of SBA-15 nanoparticles and hybrid organic-inorganic monolith.
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, PR China; Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai, PR China.
authors.forename: Zhaodi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: zhanglingyi@ecust.edu.cn.
authors.forename: Lingyi
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai, PR China.
authors.forename: Chenggong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Weibing
authors.initials: W
authors.lastname: Zhang
mesh_terms: D019149:Bioreactors; D007287:Inorganic Chemicals / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D009930:Organic Chemicals / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D007287:Inorganic Chemicals; D009930:Organic Chemicals
keywords: BSA; Cyt-C; Enzymatic reactor; Hybrid organic–inorganic monolith; IMER; MALDI-TOF-MS; RSD; SBA-15 nanoparticles; SEM; Trypsin; bovine serum albumin; cytochrome C; i.d.; immobilized enzymatic reactor; inside diameter; matrix assisted laser desorption ionization time of flight mass spectrometry; o.d.; outside diameter; relative standard deviation; scanning electron microscopy