title: BIRB 796 has Distinctive Anti-inflammatory Effects on Different Cell Types.
abstract: The pro-inflammatory cytokines tumor necrosis factor-α (TNFα) and interleukin (IL)-1β are crucial mediators involved in chronic inflammatory diseases. Inflammatory signal pathways regulate inflammatory cytokine expression-mediated by p38 mitogen activated protein kinase (p38MAPK). Therefore, considerable attention has been given to p38MAPK as a target molecule for the development of a novel anti-inflammatory therapeutics. BIRB 796, one of p38MAPK inhibitor, is a candidate of therapeutic drug for chronic inflammatory diseases. In this study, we investigated the effect of BIRB 796 on inflammatory cytokine productions by lipopolysaccharide (LPS) in different immune cell types. BIRB 796 reduced LPS-mediated IL-8 production in THP-1 cells but not in Raw 264.7 cells. Further analysis of signal molecules by western blot revealed that BIRB 796 sufficiently suppressed LPS-mediated phosphorylation of p38MAPK in both cell types whereas it failed to block inhibitor of kappa B (I-κB) degradation in Raw 264.7 cells. Taken together, these results suggest that the anti-inflammatory function of BIRB 796 depends on cell types.
authors.affiliation: Laboratory of Cytokine Immunology, Department of Biomedical Sciences and Technology, Konkuk University, Seoul 143-701, Korea. ; Division of Veterinary Bacterial Disease Division, Animal and Plant Quarantine Agency, Gyeonggi-do 480-757, Korea.
authors.forename: Soyoon
authors.initials: S
authors.lastname: Ryoo
authors.affiliation: Laboratory of Cytokine Immunology, Department of Biomedical Sciences and Technology, Konkuk University, Seoul 143-701, Korea. ; Division of Veterinary Epidemiology, Animal and Plant Quarantine Agency, Gyeonggi-do 480-757, Korea.
authors.forename: Jida
authors.initials: J
authors.lastname: Choi
authors.affiliation: Division of Veterinary Bacterial Disease Division, Animal and Plant Quarantine Agency, Gyeonggi-do 480-757, Korea.
authors.forename: Jaemyung
authors.initials: J
authors.lastname: Kim
authors.affiliation: Laboratory of Cytokine Immunology, Department of Biomedical Sciences and Technology, Konkuk University, Seoul 143-701, Korea. ; Department of Bioequivalence Division for Drug Evaluation, Ministry of Food and Drug Safety, Chungcheongbuk-do 363-700, Korea.
authors.forename: Suyoung
authors.initials: S
authors.lastname: Bae
authors.affiliation: Laboratory of Cytokine Immunology, Department of Biomedical Sciences and Technology, Konkuk University, Seoul 143-701, Korea.
authors.forename: Jaewoo
authors.initials: J
authors.lastname: Hong
authors.affiliation: Laboratory of Cytokine Immunology, Department of Biomedical Sciences and Technology, Konkuk University, Seoul 143-701, Korea.
authors.forename: Seunghyun
authors.initials: S
authors.lastname: Jo
authors.affiliation: Laboratory of Cytokine Immunology, Department of Biomedical Sciences and Technology, Konkuk University, Seoul 143-701, Korea.
authors.forename: Soohyun
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Medicine, Pusan Paik Hospital, Collage of Medicine, Inje University, Busan 633-165, Korea.
authors.forename: Youngmin
authors.initials: Y
authors.lastname: Lee
keywords: BIRB 796; Inflammatory cytokine; Inflammatory signal pathways; LPS; p38MAPK