title: Experimental lung injury promotes alterations in energy metabolism and respiratory mechanics in the lungs of rats: prevention by exercise.
abstract: In the present study we investigated the effects of lung injury on energy metabolism (succinate dehydrogenase, complex II, cytochrome c oxidase, and ATP levels), respiratory mechanics (dynamic and static compliance, elastance and respiratory system resistance) in the lungs of rats, as well as on phospholipids in bronchoalveolar lavage fluid. The protective effect of physical exercise on the alterations caused by lung injury, including lung edema was also evaluated. Wistar rats were submitted to 2 months of physical exercise. After this period the lung injury was induced by intratracheal instillation of lipopolysaccharide. Adult Wistar rats were submitted to 2 months of physical exercise and after this period the lung injury was induced by intratracheal instillation of lipopolysaccharide in dose 100 μg/100 g body weight. The sham group received isotonic saline instillation. Twelve hours after the injury was performed the respiratory mechanical and after the rats were decapitated and samples were collected. The rats subjected to lung injury presented a decrease in activities of the enzymes of the electron transport chain and ATP levels in lung, as well as the formation of pulmonary edema. A decreased lung dynamic and static compliance, as well as an increase in respiratory system resistance, and a decrease in phospholipids content were observed. Physical exercise was able to totally prevent the decrease in succinate dehydrogenase and complex II activities and the formation of pulmonary edema. It also partially prevented the increase in respiratory system resistance, but did not prevent the decrease in dynamic and static compliance, as well as in phospholipids content. These findings suggest that the mitochondrial dysfunction may be one of the important contributors to lung damage and that physical exercise may be beneficial in this pathology, although it did not prevent all changes present in lung injury.
authors.affiliation: Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, ICBS, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, Porto Alegre, RS, CEP 90035-003, Brazil.
authors.forename: Maira J
authors.initials: MJ
authors.lastname: da Cunha
authors.forename: Aline A
authors.initials: AA
authors.lastname: da Cunha
authors.forename: Emilene B S
authors.initials: EB
authors.lastname: Scherer
authors.forename: Fernanda Rossato
authors.initials: FR
authors.lastname: Machado
authors.forename: Samanta O
authors.initials: SO
authors.lastname: Loureiro
authors.forename: Rodrigo B
authors.initials: RB
authors.lastname: Jaenisch
authors.forename: Fátima
authors.initials: F
authors.lastname: Guma
authors.forename: Pedro Dal
authors.initials: PD
authors.lastname: Lago
authors.forename: Angela T S
authors.initials: AT
authors.lastname: Wyse
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D004195:Disease Models, Animal; D004579:Electron Transport / Q000187:drug effects / Q000502:physiology; D004734:Energy Metabolism / Q000187:drug effects / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology*; D055370:Lung Injury / Q000378:metabolism / Q000503:physiopathology*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D010743:Phospholipids / Q000378:metabolism; D010805:Physical Conditioning, Animal / Q000502:physiology*; D011654:Pulmonary Edema / Q000378:metabolism / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D015656:Respiratory Mechanics / Q000187:drug effects / Q000502:physiology*
chemical_list: D008070:Lipopolysaccharides; D010743:Phospholipids; D000255:Adenosine Triphosphate