title: Epigenetic effects of methoxychlor and vinclozolin on male gametes.
abstract: Imprinting is an epigenetic form of gene regulation that mediates a parent-of-origin-dependent expression of the alleles of a number of genes. Imprinting, which occurs at specific sites within or surrounding the gene, called differentially methylated domains, consists in a methylation of CpGs. The appropriate transmission of genomics imprints is essential for the control of embryonic development and fetal growth. A number of endocrine disruptors (EDs) affect male reproductive tract development and spermatogenesis. It was postulated that the genetic effects of EDs might be induced by alterations in gene imprinting. We tested two EDs: methoxychlor and vinclozolin. Their administration during gestation induced in the offspring a decrease in sperm counts and significant modifications in the methylation pattern of a selection of paternally and maternally expressed canonical imprinted genes. The observation that imprinting was largely untouched in somatic cells suggests that EDs exert their damaging effects via the process of reprogramming that is unique to gamete development. Interestingly, the effects were transgenerational, although disappearing gradually from F1 to F3. A systematic analysis showed a heterogeneity in the CpG sensitivity to EDs. We propose that the deleterious effects of EDs on the male reproductive system are mediated by imprinting defects in the sperm. The reported effects of EDs on human male spermatogenesis might be mediated by analogous imprinting alterations.
authors.affiliation: Department of Genetic Medicine and Development, University of Geneva Medical School, Geneva, Switzerland; Swiss Center for Applied Human Toxicology, University of Geneva Medical School, Geneva, Switzerland. Electronic address: ariane.giacobino@unige.ch.
authors.forename: Ariane
authors.initials: A
authors.lastname: Paoloni-Giacobino
mesh_terms: D000726:Androgen Antagonists / Q000633:toxicity; D000818:Animals; D052244:Endocrine Disruptors / Q000633:toxicity; D004781:Environmental Exposure / Q000009:adverse effects; D004785:Environmental Pollutants / Q000633:toxicity; D044127:Epigenesis, Genetic / Q000187:drug effects*; D004965:Estrogen Antagonists / Q000633:toxicity; D004968:Estrogens, Non-Steroidal / Q000633:toxicity; D005260:Female; D047109:Fetal Development / Q000187:drug effects; D005659:Fungicides, Industrial / Q000633:toxicity*; D006801:Humans; D007248:Infertility, Male / Q000139:chemically induced* / Q000196:embryology / Q000473:pathology; D007306:Insecticides / Q000633:toxicity*; D008297:Male; D008731:Methoxychlor / Q000633:toxicity*; D010080:Oxazoles / Q000633:toxicity*; D011247:Pregnancy; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000473:pathology
chemical_list: D000726:Androgen Antagonists; D052244:Endocrine Disruptors; D004785:Environmental Pollutants; D004965:Estrogen Antagonists; D004968:Estrogens, Non-Steroidal; D005659:Fungicides, Industrial; D007306:Insecticides; D010080:Oxazoles; C025643:vinclozolin; D008731:Methoxychlor
keywords: Endocrine disruptors; Epigenetics; Gametes; Infertility; Methoxychlor; Methylation; Transgenerational; Vinclozolin