title: Effects of vascular endothelial growth factor B on proliferation and migration in EA.Hy926 cells.
abstract: Vascular endothelial growth factor B (VEGF-B) was reported to be angiogenic, and it was considered as a neuroprotective agent in mouse retinal ganglion cells following optic nerve crush. Thus, it was necessary to investigate whether VEGF-B contributes to the process of retinal and choroidal neovascularization. We aimed to investigate the effects of VEGF-B on proliferation and migration in EA.Hy926 cells. The proliferation of cells was analyzed by cell counting kit 8 assay, and the migration of cells was evaluated by a modified Boyden chamber assay. The levels of phospho-ERK1/2 (P-ERK1/2), ERK1/2, phospho-p38 and p38 were detected by western blotting. The results showed that VEGF-B induced proliferation and migration of EA.Hy926 cells (P < 0.01 and P < 0.05, respectively), and ERK1/2 and p38 phosphorylation were significantly activated. Our study suggested that VEGF-B was an angiogenesis factor in vitro and that ERK1/2 and p38-related signaling pathways were involved in these VEGF-B activities.
authors.affiliation: Department of Ophthalmology, The No.474 Hospital of Chinese People's Liberation Army, Urumchi, 830011, China, zgh_hit@163.com.
authors.forename: Guang-Hong
authors.initials: GH
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Qin
authors.forename: Shui-Hua
authors.initials: SH
authors.lastname: Zhang
authors.forename: He
authors.initials: H
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D042783:Endothelial Cells / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D020935:MAP Kinase Signaling System / Q000235:genetics; D051379:Mice; D048052:Mitogen-Activated Protein Kinase 3 / Q000235:genetics; D018919:Neovascularization, Physiologic / Q000235:genetics*; D009900:Optic Nerve / Q000166:cytology / Q000378:metabolism*; D010766:Phosphorylation; D012165:Retinal Ganglion Cells / Q000378:metabolism; D042561:Vascular Endothelial Growth Factor B / Q000235:genetics* / Q000378:metabolism
chemical_list: C467485:VEGFB protein, human; D042561:Vascular Endothelial Growth Factor B; D048052:Mitogen-Activated Protein Kinase 3