title: Human papillomavirus genotypes and cervical cancer in northeast Thailand.
abstract: Human papillomavirus (HPV) is a major cause of cervical cancer. More than 100 HPV genotypes have been identified; however the distribution varies geographically and according to ethnicity. The purpose of this study was to investigate the prevalence and distribution of HPV subtypes among Northeast Thai women. Subjects included 198 cases of SCCA and 198 age-matched, healthy controls. HPV-DNA was amplified by PCR using the consensus primers GP5+/6+ system followed by reverse line blot hybridization genotyping. The prevalence of high-risk HPV infection was 21 (10.1%) and 152 (76.8%) in the controls and in the cases, respectively. High-risk HPV significantly increased the risk for cervical cancer with an OR of 42.4 (95%CI: 22.4-81.4, p<0.001) and an adjusted OR of 40.7-fold (95%CI: 21.5-76.8, p <0.001). HPV-16 was the most prevalent HPV type in the SCCA (56.2%) followed by HPV-58 (17.8%) and HPV-18 (13.6%); whereas HPV-58 (46.4%) was a prominent genotype in the controls followed by HPV-16 (39.3%) and unidentified HPV types (25.0%). These findings indicate that HPV infection remains a critical risk factor for SCCA; particularly, HPV-16, HPV-58 and HPV-18. In order to eradicate cervical cancer, sustained health education, promoted use of prophylactics and a HPV-58 vaccine should be introduced in this region.
authors.affiliation: Department of Physiology, Faculty of Public Health, Khon Kaen University, Khon Kaen, Thailand E-mail : wannapa@kku.ac.th.
authors.forename: Sitakan
authors.initials: S
authors.lastname: Natphopsuk
authors.forename: Wannapa
authors.initials: W
authors.lastname: Settheetham-Ishida
authors.forename: Chamsai
authors.initials: C
authors.lastname: Pientong
authors.forename: Supat
authors.initials: S
authors.lastname: Sinawat
authors.forename: Pissamai
authors.initials: P
authors.lastname: Yuenyao
authors.forename: Takafumi
authors.initials: T
authors.lastname: Ishida
authors.forename: Dariwan
authors.initials: D
authors.lastname: Settheetham
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology*; D016022:Case-Control Studies; D002584:Cervix Uteri / Q000378:metabolism / Q000473:pathology; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000821:virology*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D011379:Prognosis; D012307:Risk Factors; D013785:Thailand / Q000453:epidemiology; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000821:virology*; D014626:Vaginal Smears
chemical_list: D004279:DNA, Viral