title: Cytoneme-mediated contact-dependent transport of the Drosophila decapentaplegic signaling protein.
abstract: Decapentaplegic (Dpp), a Drosophila morphogen signaling protein, transfers directly at synapses made at sites of contact between cells that produce Dpp and cytonemes that extend from recipient cells. The Dpp that cytonemes receive moves together with activated receptors toward the recipient cell body in motile puncta. Genetic loss-of-function conditions for diaphanous, shibire, neuroglian, and capricious perturbed cytonemes by reducing their number or only the synapses they make with cells they target, and reduced cytoneme-mediated transport of Dpp and Dpp signaling. These experiments provide direct evidence that cells use cytonemes to exchange signaling proteins, that cytoneme-based exchange is essential for signaling and normal development, and that morphogen distribution and signaling can be contact-dependent, requiring cytoneme synapses.
authors.affiliation: Cardiovascular Research Institute, University of California, San Francisco, CA 94158, USA.
authors.forename: Sougata
authors.initials: S
authors.lastname: Roy
authors.forename: Hai
authors.initials: H
authors.lastname: Huang
authors.forename: Songmei
authors.initials: S
authors.lastname: Liu
authors.forename: Thomas B
authors.initials: TB
authors.lastname: Kornberg
mesh_terms: D000400:Air Sacs / Q000166:cytology / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism; D002450:Cell Communication; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000378:metabolism*; D034281:Dynamins / Q000235:genetics / Q000378:metabolism; D000080865:Formins; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D011554:Pseudopodia / Q000378:metabolism*; D015398:Signal Transduction; D014132:Trachea / Q000166:cytology / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D015816:Cell Adhesion Molecules, Neuronal; D029721:Drosophila Proteins; D000080865:Formins; D008565:Membrane Proteins; C113006:caps protein, Drosophila; C106890:diaphanous protein, Drosophila; C064196:dpp protein, Drosophila; C061745:Nrg protein, Drosophila; D034281:Dynamins; C439628:shi protein, Drosophila