title: [Effects of Jinmaitong capsule on oxidative stress and cell apoptosis of dorsal root ganglion in diabetic rats].
abstract: OBJECTIVE
  To study the effects of Jinmaitong capsule on oxidative stress and cell apoptosis of dorsal root ganglion (DRG) in rats with diabetic peripheral neuropathy.
  
  
  METHODS
  Sixty male SD rats were randomly divided into normal group and model groups. The diabetic rat models were established using Streptozotocin (STZ) method (60 mg/kg of intraperitoneal injection), and then randomly divided Jinmaitong low, middle, and high-dose groups and vitamin C group. All the experimental rats were sacrificed at 16-week and then the DRG was isolated. The morphological changes of DRG were observed using the Nissl's staining, and the NADPH oxidase subunit p22-phox, Cyt C, Bcl-2, and Caspase-3 of DRG in rats were detected by immunohistochemistry and quantitative reverse transcription PCR (qRT-PCR). Cell apoptosis was detected by TUNEL.
  
  
  RESULTS
  Compared with the model group, the expressions of NADPH oxidase subunit p22-phox protein, Cyt expression of C protein, Caspase-3 protein, and mRNA cell apoptosis rate in each treatment group significantly decreased whereas the expressions of Bcl-2 mRNA and protein significantly increased (P<0.05 or P<0.01). The Jinmaitong high-dose group had the best effect and was significantly different from that of the vitamin C group (P<0.01).
  
  
  CONCLUSIONS
  Jinmaitong capsule can prevent the nerve injury in rats with diabetic peripheral neuropathy by inhibiting oxidative stress and decreasing the apoptosis. The high-dose Jinmaitong capsule has the best effect and is superior to vitamin C.
authors.affiliation: Department of TCM, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of TCM, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Xiao-chun
authors.initials: XC
authors.lastname: Liang
authors.affiliation: Department of TCM, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Department of TCM, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Pu-yan
authors.initials: PY
authors.lastname: Wang
authors.affiliation: Department of TCM, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of TCM, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Wen-zhi
authors.initials: WZ
authors.lastname: Huang
authors.affiliation: Department of TCM, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Bo-wu
authors.initials: BW
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002214:Capsules; D053148:Caspase 3 / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental; D003929:Diabetic Neuropathies / Q000188:drug therapy; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D005727:Ganglia, Spinal / Q000503:physiopathology*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002214:Capsules; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; C583496:jinmaitong; D053148:Caspase 3