title: Poly(dopamine) coating to biodegradable polymers for bone tissue engineering.
abstract: In this study, a technique based on poly(dopamine) deposition to promote cell adhesion was investigated for the application in bone tissue engineering. The adhesion and proliferation of rat osteoblasts were evaluated on poly(dopamine)-coated biodegradable polymer films, such as polycaprolactone, poly(l-lactide) and poly(lactic-co-glycolic acid), which are commonly used biodegradable polymers in tissue engineering. Cell adhesion was significantly increased to a plateau by merely 15 s of dopamine incubation, 2.2-4.0-folds of increase compared to the corresponding untreated substrates. Cell proliferation was also greatly enhanced by poly(dopamine) deposition, indicated by shortened cell doubling time. Mineralization was also increased on the poly(dopamine)-deposited surfaces. The potential of poly(dopamine) deposition in bone tissue engineering is demonstrated in this study.
authors.affiliation: 1Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
authors.forename: Wei-Bor
authors.initials: WB
authors.lastname: Tsai
authors.forename: Wen-Tung
authors.initials: WT
authors.lastname: Chen
authors.forename: Hsiu-Wen
authors.initials: HW
authors.lastname: Chien
authors.forename: Wei-Hsuan
authors.initials: WH
authors.lastname: Kuo
authors.forename: Meng-Jiy
authors.initials: MJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D001842:Bone and Bones; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D007211:Indoles; D010006:Osteoblasts / Q000166:cytology; D011108:Polymers; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D020099:Coated Materials, Biocompatible; D007211:Indoles; D011108:Polymers; C568283:polydopamine
keywords: Chondrocyte; biodegradable polymers; cell attachment and proliferation; dopamine; mineralization; osteoblasts