title: Expression of N-acetylglucosaminyltransferase V in gastric cancer correlates with metastasis and prognosis.
abstract: N-acetylglucosaminyltransferase V (GnT-V) is an enzyme that catalyzes β1-6 branching of N-acetylglucosamine on asparagine (N)-linked oligosaccharides (N-glycan) of cell proteins and the dysfunction of which is a common feature of various carcinomas. Nevertheless, the role of GnT-V remains controversial. Therefore, the clinical implication of GnT-V expression may differ in each cancer type. The implication of GnT-V status in patients with gastric cancer has not been studied extensively. In the present study, we examined GnT-V expression in gastric cancer specimen both at protein and mRNA levels. We compared GnT-V expression with clinical and pathologic variables. Kaplan-Meier survival curves were generated to show the cause-specific survival. Furthermore, the small interfering RNA was devised to downregulate the GnT-V mRNA expression in SGC7901 and BGC 823 cells. We characterized the function implication of GnT-V by cell proliferation and invasiveness analysis. Analysis in gastric cancer specimen revealed that GnT-V expression correlated with tumor grade and stage. The overall survival time of positive GnT-V expression in gastric cancer was significantly shorter than that of negative GnT-V expression. Moreover, the downregulation of GnT-V expression by small interfering RNA resulted in a decrease of cell proliferation and invasiveness in SGC7901 and BGC 823 cells accompanied by morphological change. This supports that GnT-V correlates with metastasis and prognosis in gastric cancer. These results contribute to new insight into the underlying molecular mechanisms of GnT-V regulation in gastric cancer with potential translational clinical applications.
authors.affiliation: Department of Gastroenterology of Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Huang
authors.affiliation: Department of Gastroenterology of Shanghai Changning Center Hospital, Shanghai 200336, P.R. China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Gastroenterology of Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Ge
authors.affiliation: Department of Gastroenterology of Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology of Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China.
authors.forename: Longe
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Gastroenterology of Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology of Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China.
authors.forename: Liu
authors.initials: L
authors.lastname: Fu
authors.affiliation: Department of Gastroenterology of Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Gastroenterology of Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China.
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D017351:N-Acetylglucosaminyltransferases / Q000096:biosynthesis*; D009362:Neoplasm Metastasis; D011379:Prognosis; D012333:RNA, Messenger / Q000096:biosynthesis; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D012333:RNA, Messenger; D017351:N-Acetylglucosaminyltransferases; C052254:alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase