title: Experimental comparison of the high-speed imaging performance of an EM-CCD and sCMOS camera in a dynamic live-cell imaging test case.
abstract: The study of living cells may require advanced imaging techniques to track weak and rapidly changing signals. Fundamental to this need is the recent advancement in camera technology. Two camera types, specifically sCMOS and EM-CCD, promise both high signal-to-noise and high speed (>100 fps), leaving researchers with a critical decision when determining the best technology for their application. In this article, we compare two cameras using a live-cell imaging test case in which small changes in cellular fluorescence must be rapidly detected with high spatial resolution. The EM-CCD maintained an advantage of being able to acquire discernible images with a lower number of photons due to its EM-enhancement. However, if high-resolution images at speeds approaching or exceeding 1000 fps are desired, the flexibility of the full-frame imaging capabilities of sCMOS is superior.
authors.affiliation: 1th Human Performance Wing, Bioeffects Division, Air Force Research Laboratory, Fort Sam Houston, Texas, United States of America.
authors.forename: Hope T
authors.initials: HT
authors.lastname: Beier
authors.affiliation: 1th Human Performance Wing, Bioeffects Division, Air Force Research Laboratory, Fort Sam Houston, Texas, United States of America.
authors.forename: Bennett L
authors.initials: BL
authors.lastname: Ibey
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000295:instrumentation* / Q000379:methods*; D059629:Signal-To-Noise Ratio
chemical_list: D002118:Calcium