title: Carbon-flux distribution within Streptomyces coelicolor metabolism: a comparison between the actinorhodin-producing strain M145 and its non-producing derivative M1146.
abstract: Metabolic Flux Analysis is now viewed as essential to elucidate the metabolic pattern of cells and to design appropriate genetic engineering strategies to improve strain performance and production processes. Here, we investigated carbon flux distribution in two Streptomyces coelicolor A3 (2) strains: the wild type M145 and its derivative mutant M1146, in which gene clusters encoding the four main antibiotic biosynthetic pathways were deleted. Metabolic Flux Analysis and (13)C-labeling allowed us to reconstruct a flux map under steady-state conditions for both strains. The mutant strain M1146 showed a higher growth rate, a higher flux through the pentose phosphate pathway and a higher flux through the anaplerotic phosphoenolpyruvate carboxylase. In that strain, glucose uptake and the flux through the Krebs cycle were lower than in M145. The enhanced flux through the pentose phosphate pathway in M1146 is thought to generate NADPH enough to face higher needs for biomass biosynthesis and other processes. In both strains, the production of NADPH was higher than NADPH needs, suggesting a key role for nicotinamide nucleotide transhydrogenase for redox homeostasis. ATP production is also likely to exceed metabolic ATP needs, indicating that ATP consumption for maintenance is substantial.Our results further suggest a possible competition between actinorhodin and triacylglycerol biosynthetic pathways for their common precursor, acetyl-CoA. These findings may be instrumental in developing new strategies exploiting S. coelicolor as a platform for the production of bio-based products of industrial interest.
authors.affiliation: Unité Mixte de Recherche 8621, Institut de Génétique et Microbiologie, Université Paris-Sud, Orsay, France ; Centre National de la Recherche Scientifique, Orsay, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Coze
authors.affiliation: Unité Mixte de Recherche 8618, Institut de Biotechnologie des Plantes, Université Paris-Sud, Orsay, France ; Centre National de la Recherche Scientifique, Orsay, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Gilard
authors.affiliation: Unité Mixte de Recherche 8618, Institut de Biotechnologie des Plantes, Université Paris-Sud, Orsay, France ; Centre National de la Recherche Scientifique, Orsay, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Tcherkez
authors.affiliation: Unité Mixte de Recherche 8621, Institut de Génétique et Microbiologie, Université Paris-Sud, Orsay, France ; Centre National de la Recherche Scientifique, Orsay, France.
authors.forename: Marie-Joëlle
authors.initials: MJ
authors.lastname: Virolle
authors.affiliation: Unité Mixte de Recherche 8621, Institut de Génétique et Microbiologie, Université Paris-Sud, Orsay, France ; Centre National de la Recherche Scientifique, Orsay, France.
authors.forename: Armel
authors.initials: A
authors.lastname: Guyonvarch
mesh_terms: D000880:Anthraquinones / Q000378:metabolism; D018533:Biomass; D057486:Carbon Cycle; D004734:Energy Metabolism; D006706:Homeostasis; D010084:Oxidation-Reduction; D013045:Species Specificity; D048372:Streptomyces coelicolor / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D000095622:Benzoisochromanequinones
chemical_list: D000880:Anthraquinones; C013390:actinorhodin; D000095622:Benzoisochromanequinones