title: Cell-free membrane protein expression.
abstract: Cell-free protein synthesis is advantageous for membrane protein production because of the potential lethality of membrane proteins synthesized in cell-based systems. Additionally the ability to adapt the membrane composition for each membrane protein increases the likelihood of functional expression. In this chapter we describe methods for membrane protein production by the cell-free protein synthesis system assisted by detergents and lipids.
authors.affiliation: RIKEN Systems and Structural Biology Center, Tsurumi, Yokohama, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Kimura-Soyema
authors.forename: Mikako
authors.initials: M
authors.lastname: Shirouzu
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D002457:Cell Extracts; D002474:Cell-Free System; D003902:Detergents / Q000494:pharmacology; D004926:Escherichia coli / Q000166:cytology; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D008823:Micelles; D014176:Protein Biosynthesis / Q000187:drug effects; D013698:Templates, Genetic
chemical_list: D002457:Cell Extracts; D003902:Detergents; D008051:Lipid Bilayers; D008565:Membrane Proteins; D008823:Micelles