title: Haplotypes and variable position detection in the mitochondrial DNA coding region encompassing nucleotide positions 10,716-11,184.
abstract: This study evaluates the mitochondrial noncoding regions by using the Sanger sequencing method for application in Forensic Science. FTA® Technology (FTA™ paper DNA extraction) was utilized to extract DNA. Portion of coding region encompassing positions from (10,716 to 11,184) amplified in accordance with the Anderson reference sequence. PCR products purified by EZ-10 spin column were then sequenced and detected using the ABI 3730 × L DNA Analyzer. A new polymorphic positions 10,750 and 10,790 that are described may be suitable sources in future for identification purpose. The data obtained can be used to identify variable nucleotide positions characterized by frequent occurrence, most promising for identification variants.
authors.affiliation: Department of Molecular Biology, Babylon University , Hilla City , Iraq .
authors.forename: Imad Hadi
authors.initials: IH
authors.lastname: Hameed
authors.forename: Ameer Ibrahim
authors.initials: AI
authors.lastname: Abdulzahra
authors.forename: Mohammed Abdullah
authors.initials: MA
authors.lastname: Jebor
authors.forename: Cheah Yoke
authors.initials: CY
authors.lastname: Kqueen
authors.forename: Aamera Jaber
authors.initials: AJ
authors.lastname: Ommer
mesh_terms: D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000737:chemistry*; D006239:Haplotypes; D006801:Humans; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004272:DNA, Mitochondrial
keywords: Coding region; Iraq; encompassing nucleotide positions from 10,716 to 11,184; mitochondrial DNA; polymorphic positions