title: Boron concentration measurements by alpha spectrometry and quantitative neutron autoradiography in cells and tissues treated with different boronated formulations and administration protocols.
abstract: The possibility to measure boron concentration with high precision in tissues that will be irradiated represents a fundamental step for a safe and effective BNCT treatment. In Pavia, two techniques have been used for this purpose, a quantitative method based on charged particles spectrometry and a boron biodistribution imaging based on neutron autoradiography. A quantitative method to determine boron concentration by neutron autoradiography has been recently set-up and calibrated for the measurement of biological samples, both solid and liquid, in the frame of the feasibility study of BNCT. This technique was calibrated and the obtained results were cross checked with those of α spectrometry, in order to validate them. The comparisons were performed using tissues taken form animals treated with different boron administration protocols. Subsequently the quantitative neutron autoradiography was employed to measure osteosarcoma cell samples treated with BPA and with new boronated formulations.
authors.affiliation: University of Pavia, Department of Physics, Pavia 27100, Italy; National Institute for Nuclear Physics (INFN), Rome, Italy.
authors.forename: Silva
authors.initials: S
authors.lastname: Bortolussi
authors.affiliation: University of Florence, Department of Chemistry and CSGI, Florence, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Ciani
authors.affiliation: University of Pavia, Department of Physics, Pavia 27100, Italy; National Institute for Nuclear Physics (INFN), Rome, Italy.
authors.forename: Ian
authors.initials: I
authors.lastname: Postuma
authors.affiliation: University of Pavia, Department of Physics, Pavia 27100, Italy; National Institute for Nuclear Physics (INFN), Rome, Italy.
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Protti
authors.affiliation: University of Pavia, Department of Physics, Pavia 27100, Italy.
authors.lastname: Luca Reversi
authors.affiliation: University of Pavia, Department of Physics, Pavia 27100, Italy.
authors.forename: Piero
authors.initials: P
authors.lastname: Bruschi
authors.affiliation: University of Pavia, Department of Surgery, Experimental Surgery Laboratory, Pavia, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Ferrari
authors.affiliation: University of Pavia, Department of Surgery, Experimental Surgery Laboratory, Pavia, Italy; San Matteo Hospital, Foundation IRCCS, Pavia, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Cansolino
authors.affiliation: DISCAFF, University of Oriental Piemonte, Novara, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Panza
authors.affiliation: University of Florence, Department of Chemistry and CSGI, Florence, Italy.
authors.forename: Sandra
authors.initials: S
authors.lastname: Ristori
authors.affiliation: University of Pavia, Department of Physics, Pavia 27100, Italy; National Institute for Nuclear Physics (INFN), Rome, Italy.
authors.forename: Saverio
authors.initials: S
authors.lastname: Altieri
mesh_terms: D000512:Alpha Particles; D001345:Autoradiography / Q000379:methods*; D001895:Boron / Q000008:administration & dosage / Q000032:analysis*; D016754:Boron Neutron Capture Therapy / Q000379:methods*; D045744:Cell Line, Tumor; D004058:Diffusion; D006801:Humans; D007554:Isotopes / Q000008:administration & dosage / Q000032:analysis; D009502:Neutrons; D012516:Osteosarcoma / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D007554:Isotopes; D001895:Boron
keywords: BNCT; Boron measurements; Boronated liposomes