title: A new transfectant panel cell line-based MoAb-independent antigen capture assay system for detection of CD36 antibody.
abstract: OBJECTIVE
  CD36 antibody (Ab) causes several disorders: neonatal alloimmune thrombocytopenia, platelet transfusion refractoriness and non-haemolytic transfusion reactions. However, there is no gold-standard test for CD36 Ab.
  
  
  METHODS
  We developed a transfectant panel cell line-based MoAb-independent antigen capture assay system for detection of CD36 Ab and compared it with the monoclonal antibody-specific immobilization of platelet antigens (MAIPA) system in terms of sensitivity and specificity.
  
  
  RESULTS
  Our new system was characterized by (1) gene-transfected cell lines, but not panel platelets; (2) not being hampered by HLA Abs; and (3) no need to use CD36 MoAbs to ensure the antigen specificity of this detection system. In addition, it showed a much better receiver operating characteristic curve than the MAIPA system.
  
  
  CONCLUSIONS
  The present results indicate that our new system permits highly sensitive and specific detection of CD36 Ab.
authors.affiliation: Japanese Red Cross Kinki Block Blood Center, Ibaraki-City, Osaka, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Amakishi
authors.forename: T
authors.initials: T
authors.lastname: Hayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Koh
authors.forename: N
authors.initials: N
authors.lastname: Matsuyama
authors.forename: H
authors.initials: H
authors.lastname: Ishii
authors.forename: H
authors.initials: H
authors.lastname: Matsukura
authors.forename: K
authors.initials: K
authors.lastname: Yasui
authors.forename: F
authors.initials: F
authors.lastname: Hirayama
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry*; D016824:Antigens, Human Platelet / Q000276:immunology; D001323:Autoantibodies / Q000097:blood* / Q000302:isolation & purification; D018955:CD36 Antigens / Q000276:immunology*; D002460:Cell Line; D006801:Humans; D012372:ROC Curve; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; D001323:Autoantibodies; D018955:CD36 Antigens
keywords: CD36 antibody; MAIPA; ROC curve; panel cell line; transfectant