title: An integrated microfluidic device for rapid serodiagnosis of amebiasis.
abstract: A microfluidic device was successfully fabricated for the rapid serodiagnosis of amebiasis. A micro bead-based immunoassay was fabricated within integrated microfluidic chip to detect the antibody to Entamoeba histolytica in serum samples. In this assay, a recombinant fragment of C terminus of intermediate subunit of galactose and N-acetyl-D-galactosamine-inhibitable lectin of Entamoeba histolytica (C-Igl, aa 603-1088) has been utilized instead of the crude antigen. This device was validated with serum samples from patients with amebiasis and showed great sensitivity. The serodiagnosis can be completed within 20 min with 2 μl sample consumption. The device can be applied for the rapid and cheap diagnosis of other infectious disease, especially for the developing countries with very limited medical facilities.
authors.affiliation: Department of Environmental Science and Engineering, Fudan University, 220 Handan Road, Shanghai 200433, People's Republic of China.
authors.forename: Wang
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Medical Microbiology and Parasitology, Medical Centre of Fudan University, Shanghai, 200032, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Environmental Science and Engineering, Fudan University, 220 Handan Road, Shanghai 200433, People's Republic of China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Jing
authors.affiliation: Department of Environmental Science and Engineering, Fudan University, 220 Handan Road, Shanghai 200433, People's Republic of China.
authors.forename: Sixiu
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Infectious Diseases, School of Medicine, Tokai University, Isehara, Kanagawa 259-1193, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tachibana
authors.affiliation: Department of Medical Microbiology and Parasitology, Medical Centre of Fudan University, Shanghai, 200032, People's Republic of China.
authors.forename: Xunjia
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Department of Environmental Science and Engineering, Fudan University, 220 Handan Road, Shanghai 200433, People's Republic of China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Sui