title: TIAM2 enhances non-small cell lung cancer cell invasion and motility.
abstract: BACKGROUND
  TIAM2, a Rac guanine nucleotide exchange factor, is closely associated with cell adherence and migration. Here, we aimed to investigate the role of TIAM2 in non-small cell lung cancer (NSCLC) cells.
  
  
  METHODS
  A small interference RNA (siRNA) was introduced to silence the expression of TIAM2. Invasion and motility assays were then performed to assess the invasion and motility potential of NSCLC cells. GST-pull down assays were used to detect activation of Rac1.
  
  
  RESULTS
  TIAM2 was highly expressed in NSCLC cells. Knockdown of TIAM2 inhibited the invasion and motility, and suppressed activation of Rac1. Further experiments demonstrated that knockdown of TIAM2 could up-regulate the expression of E-cadherin, and down- regulate the expression of MMP-3, Twist and Snail.
  
  
  CONCLUSIONS
  Our data suggest that TIAM2 can promote invasion and motility of NSCLC cells. Activation of Rac1 and regulation of some EMT/invasion-related genes may be involved in the underlying processes.
authors.affiliation: Department of General Thoracic Surgery, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces, Tianjin, China E-mail : zyzhao55@163.com.
authors.forename: Zheng-Yuan
authors.initials: ZY
authors.lastname: Zhao
authors.forename: Chen-Guang
authors.initials: CG
authors.lastname: Han
authors.forename: Jun-Tao
authors.initials: JT
authors.lastname: Liu
authors.forename: Chang-Lei
authors.initials: CL
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Li-Ya
authors.initials: LY
authors.lastname: Cheng
mesh_terms: D015820:Cadherins / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D015536:Down-Regulation; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D020662:Guanine Nucleotide Exchange Factors / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D009361:Neoplasm Invasiveness; D015854:Up-Regulation; D020830:rac1 GTP-Binding Protein / Q000096:biosynthesis / Q000235:genetics
chemical_list: D015820:Cadherins; D020662:Guanine Nucleotide Exchange Factors; C107953:RAC1 protein, human; C122170:TIAM2 protein, human; D020830:rac1 GTP-Binding Protein