title: Histological and enzymatic responses of Japanese flounder (Paralichthys olivaceus) and its hybrids (P. olivaceus ♀ × P. dentatus ♂) to chronic heat stress.
abstract: This study investigated the effects of long-term heat exposure on Japanese flounder (Paralichthys olivaceus) and its hybrids (P. olivaceus ♀ × summer flounder Paralichthys dentatus ♂). From 24 ± 0.5°C, temperature was increased by 1 ± 0.5°C in a day and was kept at that temperature for 5 days before next rise. Cumulative survival rate (CSR), cumulative survival rate under different temperature (CSR-T), histological alteration, and related enzyme activities were investigated. In P. olivaceus, mass mortality occurred at 29 and 32 °C (the CSR-T dropped to 42.39%), and serious gill damages appeared at 30 and 32°C. Meanwhile, the activities of superoxide dismutase (SOD), catalase (CAT), lysozyme (LZM), and pyruvate kinase (PK) declined around 29 and 32°C (except for CAT). In comparison with P. olivaceus, the CSR of the hybrids was higher, the gill kept a better structural integrity, and the activities of SOD, CAT, LZM, and PK showed tiny fluctuations. The results suggested that during the process of chronic heat stress, P. olivaceus seemed to be more sensitive to 29 and 32°C, and the manifestations in survival, histology, and enzyme activity were generally consistent. For the hybrids, the comparatively insensitivity to high temperature might imply its better heat tolerance.
authors.affiliation: Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Liu
authors.forename: Daoyuan
authors.initials: D
authors.lastname: Ma
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhao
authors.forename: Wenqi
authors.initials: W
authors.lastname: Wang
authors.forename: Xuelei
authors.initials: X
authors.lastname: Zhang
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Xiao
authors.forename: Shihong
authors.initials: S
authors.lastname: Xu
authors.forename: Yongshuang
authors.initials: Y
authors.lastname: Xiao
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Li
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002374:Catalase / Q000378:metabolism; D005393:Fish Diseases / Q000201:enzymology* / Q000401:mortality / Q000503:physiopathology*; D005432:Flounder / Q000235:genetics*; D005880:Gills / Q000473:pathology; D018882:Heat Stress Disorders / Q000201:enzymology / Q000401:mortality / Q000503:physiopathology / Q000662:veterinary*; D006824:Hybridization, Genetic; D009113:Muramidase / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000378:metabolism; D016019:Survival Analysis; D013696:Temperature
chemical_list: D002374:Catalase; D013482:Superoxide Dismutase; D011770:Pyruvate Kinase; D009113:Muramidase