title: Dynamic chemotactic response of fibroblasts to local stimulation using EGF-immobilized microbeads.
abstract: Directional cellular migrations as a chemotactic response to spatially inhomogeneous growth factor stimulation play an important role in establishing physiological mechanisms and pathological events in cells. We developed epidermal growth factor (EGF)-immobilized microbeads by photoreaction and evaluated its local stimulatory effects on the dynamic chemotactic motility of fibroblasts. The local stimulation resulted in global activation of ERK 1/2 and directionality of cellular migration. The cellular migration by stimulation using 3-μm diameter EGF-immobilized microbeads persisted for a longer time, were involved a wider field and their number were further increased with stimulation. This effective technique allows cellular migration and biochemical analyses that will help elucidate the mechanisms involved in signal transduction by spatially inhomogeneous stimulation of the growth factor.
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, J3-162, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Aratsu
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, J3-162, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan. Electronic address: iharada@bio.titech.ac.jp.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Harada
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, J3-162, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Soichiro
authors.initials: S
authors.lastname: Yoshimura
authors.affiliation: Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, 151-921, Republic of Korea.
authors.forename: Chong-Su
authors.initials: CS
authors.lastname: Cho
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, J3-162, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Akaike
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, J3-162, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Yoh-ichi
authors.initials: Y
authors.lastname: Tagawa
mesh_terms: D000818:Animals; D002633:Chemotaxis / Q000187:drug effects*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects*; D015151:Immunoblotting; D051379:Mice; D008863:Microspheres; D015398:Signal Transduction; D041701:Swiss 3T3 Cells
chemical_list: D004815:Epidermal Growth Factor
keywords: Cellular migration; Chemotactic response; Epidermal growth factor; Immobilized microbead; Local stimulation