title: [Expression of growth-associated protein 43 in the hippocampus of mesial temporal lobe epilepsy mouse model].
abstract: OBJECTIVE
  To explore the temporal and spatial distribution of growth-associated protein 43(GAP-43)and phosphorylated growth-associated protein 43(p-GAP-43)in the dentate gyrus of mesial temporal lobe epilepsy(MTLE)mouse model.
  
  
  METHODS
  MTLE mouse model was established by using the kainic acid(KA)induction. Immunohistochemistry and Western blotting were applied to detect the expressions of GAP-43 and p-GAP-43 in different stages of epileptogenesis.
  
  
  RESULTS
  Both in the epileptic and control mice, high GAP-43 expression level was detected in the dentate gyrus, hilus, and inner molecular layer of hippocampus. Decreased p-GAP-43 expression was detected 5 days, 2 weeks, and 5 weeks after KA-induced seizures.
  
  
  CONCLUSIONS
  The decreased p-GAP-43 expression in the duration of seizure may play an important role in the synaptic reorganization of the sclerotic hippocampus.
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Xiao-feng
authors.initials: XF
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Long-ze
authors.initials: LZ
authors.lastname: Sha
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Sha
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Xu
mesh_terms: D000818:Animals; D018891:Dentate Gyrus / Q000378:metabolism; D004195:Disease Models, Animal; D004827:Epilepsy; D004833:Epilepsy, Temporal Lobe / Q000378:metabolism*; D019922:GAP-43 Protein / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D007608:Kainic Acid; D051379:Mice; D012640:Seizures
chemical_list: D019922:GAP-43 Protein; D007608:Kainic Acid