title: Combination of Entner-Doudoroff pathway with MEP increases isoprene production in engineered Escherichia coli.
abstract: Embden-Meyerhof pathway (EMP) in tandem with 2-C-methyl-D-erythritol 4-phosphate pathway (MEP) is commonly used for isoprenoid biosynthesis in E. coli. However, this combination has limitations as EMP generates an imbalanced distribution of pyruvate and glyceraldehyde-3-phosphate (G3P). Herein, four glycolytic pathways-EMP, Entner-Doudoroff Pathway (EDP), Pentose Phosphate Pathway (PPP) and Dahms pathway were tested as MEP feeding modules for isoprene production. Results revealed the highest isoprene production from EDP containing modules, wherein pyruvate and G3P were generated simultaneously; isoprene titer and yield were more than three and six times higher than those of the EMP module, respectively. Additionally, the PPP module that generates G3P prior to pyruvate was significantly more effective than the Dahms pathway, in which pyruvate production precedes G3P. In terms of precursor generation and energy/reducing-equivalent supply, EDP+PPP was found to be the ideal feeding module for MEP. These findings may launch a new direction for the optimization of MEP-dependent isoprenoid biosynthesis pathways.
authors.affiliation: Energy and Environment Fusion Technology Center, Department of Energy and Biotechnology, Myongji University, Cheoin-gu, Yongin-si, Gyeonggi-do, Republic of Korea.
authors.forename: Huaiwei
authors.initials: H
authors.lastname: Liu
authors.affiliation: Energy and Environment Fusion Technology Center, Department of Energy and Biotechnology, Myongji University, Cheoin-gu, Yongin-si, Gyeonggi-do, Republic of Korea.
authors.forename: Yuanzhang
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Energy and Environment Fusion Technology Center, Department of Energy and Biotechnology, Myongji University, Cheoin-gu, Yongin-si, Gyeonggi-do, Republic of Korea.
authors.forename: Kristine Rose M
authors.initials: KR
authors.lastname: Ramos
authors.affiliation: Energy and Environment Fusion Technology Center, Department of Energy and Biotechnology, Myongji University, Cheoin-gu, Yongin-si, Gyeonggi-do, Republic of Korea.
authors.forename: Grace M
authors.initials: GM
authors.lastname: Nisola
authors.affiliation: Energy and Environment Fusion Technology Center, Department of Energy and Biotechnology, Myongji University, Cheoin-gu, Yongin-si, Gyeonggi-do, Republic of Korea.
authors.forename: Kris Niño G
authors.initials: KN
authors.lastname: Valdehuesa
authors.affiliation: Division of Bioscience and Bioinformatics, Myongji University, Cheoin-gu, Yongin-si, Gyeonggi-do, Republic of Korea.
authors.forename: Won-Keun
authors.initials: WK
authors.lastname: Lee
authors.affiliation: Energy and Environment Fusion Technology Center, Department of Energy and Biotechnology, Myongji University, Cheoin-gu, Yongin-si, Gyeonggi-do, Republic of Korea.
authors.forename: Si Jae
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Energy and Environment Fusion Technology Center, Department of Energy and Biotechnology, Myongji University, Cheoin-gu, Yongin-si, Gyeonggi-do, Republic of Korea.
authors.forename: Wook-Jin
authors.initials: WJ
authors.lastname: Chung
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002070:Butadienes; D004896:Erythritol / Q000031:analogs & derivatives / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005986:Glyceraldehyde 3-Phosphate / Q000378:metabolism; D006019:Glycolysis / Q000235:genetics*; D045782:Hemiterpenes / Q000096:biosynthesis*; D060847:Metabolic Engineering; D010420:Pentanes; D010957:Plasmids; D019289:Pyruvic Acid / Q000378:metabolism; D013403:Sugar Phosphates / Q000378:metabolism
chemical_list: C114232:2-C-methylerythritol 4-phosphate; D001426:Bacterial Proteins; D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; D013403:Sugar Phosphates; C005059:isoprene; D005986:Glyceraldehyde 3-Phosphate; D019289:Pyruvic Acid; D004896:Erythritol