title: Prevalence and persistence of potentially pathogenic and antibiotic resistant bacteria during anaerobic digestion treatment of cattle manure.
abstract: Anaerobic digestion figures as a sustainable alternative to avoid discharge of cattle manure in the environment, which results in biogas and biofertilizer. Persistence of potentially pathogenic and drug-resistant bacteria during anaerobic digestion of cattle manure was evaluated. Selective cultures were performed for enterobacteria (ENT), non-fermenting Gram-negative rods (NFR) and Gram-positive cocci (GPC). Antimicrobial susceptibility patterns were determined and a decay of all bacterial groups was observed after 60days. Multidrug-resistant bacteria were detected both the influent and effluent. GPC, the most prevalent group was highly resistant against penicillin and levofloxacin, whereas resistance to ampicillin, ampicillin-sulbactam and chloramphenicol was frequently observed in the ENT and NFR groups. The data point out the need of discussions to better address management of biodigesters and the implementation of sanitary and microbiological safe treatments of animal manures to avoid consequences to human, animal and environmental health.
authors.affiliation: Department of Parasitology, Microbiology and Immunology, Institute of Biological Sciences, Federal University of Juiz de Fora, 36036-900 Juiz de Fora, MG, Brazil.
authors.forename: Juliana Alves
authors.initials: JA
authors.lastname: Resende
authors.affiliation: Department of Parasitology, Microbiology and Immunology, Institute of Biological Sciences, Federal University of Juiz de Fora, 36036-900 Juiz de Fora, MG, Brazil.
authors.forename: Vânia Lúcia
authors.initials: VL
authors.lastname: Silva
authors.affiliation: Department of Parasitology, Microbiology and Immunology, Institute of Biological Sciences, Federal University of Juiz de Fora, 36036-900 Juiz de Fora, MG, Brazil.
authors.forename: Tamara Lopes Rocha
authors.initials: TL
authors.lastname: de Oliveira
authors.affiliation: Department of Parasitology, Microbiology and Immunology, Institute of Biological Sciences, Federal University of Juiz de Fora, 36036-900 Juiz de Fora, MG, Brazil.
authors.forename: Samuel
authors.initials: S
authors.lastname: de Oliveira Fortunato
authors.affiliation: Embrapa Dairy Cattle, Research Center, 36038-330 Juiz de Fora, MG, Brazil.
authors.forename: Jailton
authors.initials: J
authors.lastname: da Costa Carneiro
authors.affiliation: Embrapa Dairy Cattle, Research Center, 36038-330 Juiz de Fora, MG, Brazil.
authors.forename: Marcelo Henrique
authors.initials: MH
authors.lastname: Otenio
authors.affiliation: Department of Parasitology, Microbiology and Immunology, Institute of Biological Sciences, Federal University of Juiz de Fora, 36036-900 Juiz de Fora, MG, Brazil. Electronic address: claudio.diniz@ufjf.edu.br.
authors.forename: Cláudio Galuppo
authors.initials: CG
authors.lastname: Diniz
mesh_terms: D000693:Anaerobiosis / Q000187:drug effects; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000254:growth & development* / Q000302:isolation & purification; D001673:Biodegradation, Environmental / Q000187:drug effects; D056804:Biofuels / Q000382:microbiology; D019149:Bioreactors / Q000382:microbiology; D002417:Cattle; D015169:Colony Count, Microbial; D004352:Drug Resistance, Microbial; D006801:Humans; D008372:Manure / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D010865:Pilot Projects; D012037:Refuse Disposal / Q000379:methods*; D013045:Species Specificity
chemical_list: D000900:Anti-Bacterial Agents; D056804:Biofuels; D008372:Manure
keywords: Anaerobic digestion; Antimicrobial resistance; Biofertilizer dairy cattle manure; Biogas; Pathogenic bacteria