title: Synthesis of γ-labeled nucleoside 5'-triphosphates using click chemistry.
abstract: Real-time enzymatic studies are gaining importance as their chemical and technical instrumentation improves. Here we report the efficient synthesis of γ-alkyne modified triphosphate amidates that are converted into a variety of γ-fluorophore labeled triphosphates by Cu(I) catalyzed alkyne/azide click reactions. The synthesized triphosphates are incorporated into DNA by DNA polymerases.
authors.affiliation: Department of Chemistry, Ludwig-Maximilians Universität München, Butenandtstraße 5-13, 81377 Munich, Germany. thomas.carell@cup.uni-muenchen.de.
authors.forename: S
authors.initials: S
authors.lastname: Serdjukow
authors.forename: F
authors.initials: F
authors.lastname: Kink
authors.forename: B
authors.initials: B
authors.lastname: Steigenberger
authors.forename: M
authors.initials: M
authors.lastname: Tomás-Gamasa
authors.forename: T
authors.initials: T
authors.lastname: Carell
mesh_terms: D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D001483:Base Sequence; D002384:Catalysis; D057930:Click Chemistry; D003300:Copper / Q000737:chemistry; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics; D009711:Nucleotides / Q000737:chemistry*; D011122:Polyphosphates / Q000138:chemical synthesis* / Q000737:chemistry*
chemical_list: D000480:Alkynes; D001386:Azides; D009711:Nucleotides; D011122:Polyphosphates; D003300:Copper; D004247:DNA; C005692:triphosphoric acid