title: [Study on RSLC fingerprint of Lycii cortex].
abstract: OBJECTIVE
  To develope a method of RSLC fingerprint analysis for the quality evaluation of Lycii Cortex, and assess the differences of Lycii Cortex from Lycium chinense and L. barbarum.
  
  
  METHODS
  All separations were performed using a Rapid Separation Liquid Chromatography (RSLC) system with an Acquity UPLC C18 (2.1 mm x 100 mm, 1.7 microm) column. The mobile phase was consisted of acetonitrile and 0.1% aqueous acetic acid. The flow rate was 0.3 mL/min using gradient elution. The column temperature was 40 degrees C and detection wavelength was set at 315 nm. Lycii Cortex samples collected from different habitats were assessed by similarity analysis (SA), hierarchical clustering analysis (HCA) and principle component analysis (PCA).
  
  
  RESULTS
  The characteristic RSLC fingerprint was established with 14 common peaks. 14 hatches of samples were classified as 2 clusters by HCA and PCA, and the result demonstrated that there were differences in the contents of chemical composition between 2 species of Lycii Cortex.
  
  
  CONCLUSIONS
  The established RSLC fingerprint is specific and the method is rapid, simple and reliable, which can be used to control the quality of Lycii Cortex in the markets.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Peking Union Medical College/Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100193, China. zhaoxiaoling11@sina.com
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Zhao
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Peking Union Medical College/Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100193, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Yao
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Peking Union Medical College/Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100193, China.
authors.forename: Xin-Yao
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Peking Union Medical College/Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100193, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Ma
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Peking Union Medical College/Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100193, China.
authors.forename: Chun-Nian
authors.initials: CN
authors.lastname: He
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Peking Union Medical College/Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100193, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Peng
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016000:Cluster Analysis; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D017753:Ecosystem; D032305:Lycium / Q000737:chemistry* / Q000254:growth & development; D024301:Plant Bark / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry*; D025341:Principal Component Analysis; D011786:Quality Control
chemical_list: D004365:Drugs, Chinese Herbal