title: Characterization of an invertebrate-type dopamine receptor of the American cockroach, Periplaneta americana.
abstract: We have isolated a cDNA coding for a putative invertebrate-type dopamine receptor (Peadop2) from P. americana brain by using a PCR-based strategy. The mRNA is present in samples from brain and salivary glands. We analyzed the distribution of the PeaDOP2 receptor protein with specific affinity-purified polyclonal antibodies. On Western blots, PeaDOP2 was detected in protein samples from brain, subesophageal ganglion, thoracic ganglia, and salivary glands. In immunocytochemical experiments, we detected PeaDOP2 in neurons with their somata being located at the anterior edge of the medulla bilaterally innervating the optic lobes and projecting to the ventro-lateral protocerebrum. In order to determine the functional and pharmacological properties of the cloned receptor, we generated a cell line constitutively expressing PeaDOP2. Activation of PeaDOP2-expressing cells with dopamine induced an increase in intracellular cAMP. In contrast, a C-terminally truncated splice variant of this receptor did not exhibit any functional property by itself. The molecular and pharmacological characterization of the first dopamine receptor from P. americana provides the basis for forthcoming studies focusing on the significance of the dopaminergic system in cockroach behavior and physiology.
authors.affiliation: Institute of Biochemistry and Biology, University of Potsdam, Potsdam D-14476, Germany. b.troppmann@web.de.
authors.forename: Britta
authors.initials: B
authors.lastname: Troppmann
authors.affiliation: Institute of Biochemistry and Biology, University of Potsdam, Potsdam D-14476, Germany. s.balfanz@fz-juelich.de.
authors.forename: Sabine
authors.initials: S
authors.lastname: Balfanz
authors.affiliation: Institute of Biochemistry and Biology, University of Potsdam, Potsdam D-14476, Germany. Christian.Krach@gmx.de.
authors.forename: Christian
authors.initials: C
authors.lastname: Krach
authors.affiliation: Institute of Biochemistry and Biology, University of Potsdam, Potsdam D-14476, Germany. a.baumann@fz-juelich.de.
authors.forename: Arnd
authors.initials: A
authors.lastname: Baumann
authors.affiliation: Institute of Biochemistry and Biology, University of Potsdam, Potsdam D-14476, Germany. Blenau@bio.uni-frankfurt.de.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Blenau
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D030541:Databases, Genetic; D018491:Dopamine Agonists / Q000737:chemistry / Q000378:metabolism; D018492:Dopamine Antagonists / Q000737:chemistry / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D010526:Periplaneta / Q000378:metabolism*; D010802:Phylogeny; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism; D011954:Receptors, Dopamine / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D018491:Dopamine Agonists; D018492:Dopamine Antagonists; D012333:RNA, Messenger; D011954:Receptors, Dopamine; D000242:Cyclic AMP