title: Molecular cloning and heterologous expression in Pichia pastoris of X-prolyl-dipeptidyl aminopeptidase from basidiomycete Ustilago maydis.
abstract: Dipeptidyl aminopeptidases are enzymes involved in the posttranslational control of bioactive peptides. Here we identified the gene dapUm in Ustilago maydis by homology with other fungal dipeptidyl aminopeptidases. Analysis of the dapUm-deduced amino acid sequence indicated that it encodes for membrane-type serine protease with a characteristic prolyl oligopeptidase catalytic motif triad: Ser, Asp, His. In order to overexpress the DapUm, the gene encoding for it was cloned and transformed into Pichia. Using this system, we observed a ∼ 125-kDa recombinant protein with an optimal enzymatic activity at pH 6.0 and at 40 °C for the Ala-Pro-p-nitroanilide substrate and an experimental pH of 6.9. U. maydis DapUm was specifically inhibited by phenylmethylsulfonyl fluoride and Pefabloc, confirming the presence of a serine residue in the active site. To our knowledge, this study is the first report on the cloning and expression of a DPP IV dipeptidyl aminopeptidase from a basidiomycete organism. Moreover, the use of recombinant DapUm will allow us to further study and characterize this enzyme, in addition to testing chemical compounds for pharmaceutical purposes.
authors.affiliation: Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Plan de Ayala y Prol. Carpio, Col. Casco de Santo Tomás, Agencia de Correos 220, México D. F., 11340, Mexico.
authors.forename: Margarita
authors.initials: M
authors.lastname: Juárez-Montiel
authors.forename: J Antonio
authors.initials: JA
authors.lastname: Ibarra
authors.forename: Griselda
authors.initials: G
authors.lastname: Chávez-Camarillo
authors.forename: César
authors.initials: C
authors.lastname: Hernández-Rodríguez
authors.forename: Lourdes
authors.initials: L
authors.lastname: Villa-Tanaca
mesh_terms: D020816:Amino Acid Motifs; D003001:Cloning, Molecular; D004151:Dipeptides / Q000737:chemistry; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000737:chemistry; D005656:Fungal Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008969:Molecular Sequence Data; D010664:Phenylmethylsulfonyl Fluoride / Q000737:chemistry; D010843:Pichia / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D013450:Sulfones / Q000737:chemistry; D014588:Ustilago / Q000737:chemistry* / Q000201:enzymology
chemical_list: D004151:Dipeptides; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D011994:Recombinant Proteins; D013450:Sulfones; C002010:4-(2-aminoethyl)benzenesulfonylfluoride; D010664:Phenylmethylsulfonyl Fluoride; C083013:alanylproline-4-nitroanilide; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; C098392:PepX dipeptidyl aminopeptidase