title: Long-term effects of magnetically targeted ferumoxide-labeled human neural stem cells in focal cerebral ischemia.
abstract: The long-term effect of magnetically targeted neural stem cells in a rat focal cerebral ischemia model was investigated. In middle cerebral artery occlusion (MCAO) stroke model rats, ferumoxide-labeled human neural stem cells (hNSCs) were injected into the tail vein. MCAO rats were divided into three groups: ischemia only (IO), ischemia with NSC injection (IC), and ischemia with NSC injection and the use of magnet targeting (IM). Four weeks after MCAO and 3 weeks posttransplantation, a greater number of hNSCs were found in ischemic lesion sites in IM rat brain compared with IO and IC animals. In addition, differentiation of hNSCs into neurons or astrocytes and angiogenesis were markedly increased. In IM rats, infarct volume was considerably reduced, and function was significantly improved. The present study indicates that long-term use of magnetic fields may be a useful way to improve the efficacy of targeted migration of stem cells and functional deficits in stem cell-based therapy for ischemic brain injury.
authors.affiliation: Department of Neurology, Clinical Research Institute, Seoul National University Hospital, Seoul, Korea.
authors.forename: Miyeoun
authors.initials: M
authors.lastname: Song
authors.forename: Young-Ju
authors.initials: YJ
authors.lastname: Kim
authors.forename: Yoon-Ha
authors.initials: YH
authors.lastname: Kim
authors.forename: Jina
authors.initials: J
authors.lastname: Roh
authors.forename: Eun-Cheol
authors.initials: EC
authors.lastname: Kim
authors.forename: Hong Jun
authors.initials: HJ
authors.lastname: Lee
authors.forename: Seung U
authors.initials: SU
authors.lastname: Kim
authors.forename: Byung-Woo
authors.initials: BW
authors.lastname: Yoon
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D001921:Brain / Q000473:pathology; D002545:Brain Ischemia / Q000473:pathology / Q000628:therapy*; D002460:Cell Line; D003911:Dextrans / Q000737:chemistry*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008297:Male; D058953:Neural Stem Cells / Q000737:chemistry / Q000166:cytology / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D014183:Transplantation, Heterologous
chemical_list: D003911:Dextrans; D058185:Magnetite Nanoparticles; C065507:ferumoxides