title: High frequency of Acinetobacter soli among Acinetobacter isolates causing bacteremia at a tertiary hospital in Japan.
abstract: Acinetobacter baumannii is generally the most frequently isolated Acinetobacter species. Sequence analysis techniques allow reliable identification of Acinetobacter isolates at the species level. Forty-eight clinical isolates of Acinetobacter spp. were obtained from blood cultures at Tohoku University Hospital. These isolates were identified at the species level by partial sequencing of the RNA polymerase β-subunit (rpoB), 16S rRNA, and gyrB genes. Then further characterization was done by using the PCR for detection of OXA-type β-lactamase gene clusters, metallo-β-lactamases, and carO genes. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing were also performed. The most frequent isolate was Acinetobacter soli (27.1%). Six of the 13 A. soli isolates were carbapenem nonsusceptible, and all of these isolates produced IMP-1. PFGE revealed that the 13 A. soli isolates were divided into 8 clusters. This study demonstrated that A. soli accounted for a high proportion of Acinetobacter isolates causing bacteremia at a Japanese tertiary hospital. Non-A. baumannii species were identified more frequently than A. baumannii and carbapenem-nonsusceptible isolates were found among the non-A. baumannii strains. These results emphasize the importance of performing epidemiological investigations of Acinetobacter species.
authors.affiliation: Department of Infection Control and Laboratory Diagnostics, Internal Medicine, Tohoku University Graduate School of Medicine, Seiryo-machi, Aoba-ku, Sendai, Miyagi, Japan.
authors.forename: Shiro
authors.initials: S
authors.lastname: Endo
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Yano
authors.forename: Hajime
authors.initials: H
authors.lastname: Kanamori
authors.forename: Shinya
authors.initials: S
authors.lastname: Inomata
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Aoyagi
authors.forename: Masumitsu
authors.initials: M
authors.lastname: Hatta
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Gu
authors.forename: Koichi
authors.initials: K
authors.lastname: Tokuda
authors.forename: Miho
authors.initials: M
authors.lastname: Kitagawa
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Kaku
mesh_terms: D000150:Acinetobacter / Q000145:classification / Q000302:isolation & purification*; D000151:Acinetobacter Infections / Q000453:epidemiology* / Q000382:microbiology*; D016470:Bacteremia / Q000453:epidemiology* / Q000382:microbiology*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001769:Blood / Q000382:microbiology; D016000:Cluster Analysis; D003428:Cross Infection / Q000453:epidemiology* / Q000382:microbiology*; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D007564:Japan / Q000453:epidemiology; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D015995:Prevalence; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D062606:Tertiary Care Centers; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D001618:beta-Lactamases; D027081:DNA Gyrase