title: Role of insulin-like growth factor II receptor in transdifferentiation of free silica-induced primary rat lung fibroblasts.
abstract: OBJECTIVE
  To study the role of insulin-like growth factor II receptor in free silica-induced transdifferentiation of primary rat lung fibroblasts.
  
  
  METHODS
  Rat lung fibroblasts and rat alveolar macrophages were cultured. A transdifferentiation model of primary rat lung fibroblasts was induced by free silica. Levels of α-SMA protein, IGF-IIR protein and mRNA were measured by immunocytochemistry, Western blot and RT-PCR, respectively. Lung fibroblasts were treated with Wortmannin.
  
  
  RESULTS
  The expression levels of α-SMA and IGF-IIR increased with the increasing free silica concentration and decreased after Wortmannin was used.
  
  
  CONCLUSIONS
  The IGF-IIR plays an important role in free silica-induced transdifferentiation of primary rat lung fibroblasts.
authors.affiliation: Department of Occupational Health and Occupational Disease, College of Public Health, Zhengzhou University, Zhengzhou 450001, Henan, China.
authors.forename: Chang Fu
authors.initials: CF
authors.lastname: Hao
authors.forename: Xiao Fang
authors.initials: XF
authors.lastname: Li
authors.forename: Wu
authors.initials: W
authors.lastname: Yao
mesh_terms: D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D017931:DNA Primers; D005347:Fibroblasts / Q000187:drug effects; D008168:Lung / Q000166:cytology / Q000187:drug effects*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D017527:Receptor, IGF Type 2 / Q000235:genetics / Q000502:physiology*; D012822:Silicon Dioxide / Q000494:pharmacology*
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D017527:Receptor, IGF Type 2; D012822:Silicon Dioxide
keywords: Insulin-like growth factor II receptor; Lung fibroblasts; Silicosis; Transdifferentiation