title: Acetylpuerarin reduces inflammation and improves memory function in a rat model of Alzheimer's disease induced by Abeta1-42.
abstract: This study was performed to determine if acetylpuerarin (compound N-2211) could reduce amyloid-beta1-42 (Abeta1-42) induced learning and memory deficits and to examine its anti-neuroinflammatory effects in a rat model. Forty Wistar rats were randomly divided into four groups (n = 10 each): control, model (Abeta1-42 injected), low-dose and high-dose acetylpuerarin groups. The acetylpuerarin groups received peritoneal acetylpuerarin every day for 12 days after 2 weeks of Abeta1-42 (5 microg/1 microl) intrahippocampal injections. The Morris water maze (MWM) was used to assess rats' learning and memory abilities. Immunohistochemistry was used to assess expression levels of ionized calcium-binding adaptor molecule (Ibal), protein kinase C delta (PKCdelta), IkappaB kinase beta (IKKbeta), and inducible nitric oxide synthase (iNOS) in hippocampus. After Abeta1-42 injection, the learning and memory abilities of rats were reduced, and acetylpuerarin treatment ameliorated the observed deficits. Abeta1-42 injection resulted in microglia transforming from resting microglia into an activated state, but this was reduced by acetylpuerarin treatment. Furthermore, hippocampal expression of PKCdelta, IKKbeta, and iNOS increased following Abeta1-42 treatment, and acetylpuerarin could suppressed the levels of PKCdelta, iNOS, and IKKbeta. Acetylpuerarin improves learning and memory functions in Abeta1-42 induced rat models. These effects may be due to anti-neuroinflammatory effects.
authors.affiliation: Shandong University School of Nursing, Jinan, Shandong, P.R. China. mqhsdu2012@163.com
authors.forename: Q H
authors.initials: QH
authors.lastname: Meng
authors.affiliation: Shandong University School of Nursing, Jinan, Shandong, P.R. China.
authors.forename: F L
authors.initials: FL
authors.lastname: Lou
authors.affiliation: Weifang Medical University College of Nursing, Weifang, Shandong, P.R. China.
authors.forename: W X
authors.initials: WX
authors.lastname: Hou
authors.affiliation: Weifang Medical University College of Nursing, Weifang, Shandong, P.R. China.
authors.forename: M
authors.initials: M
authors.lastname: Liu
authors.affiliation: Weifang Medical University College of Nursing, Weifang, Shandong, P.R. China.
authors.forename: H
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Pharmacology, Shandong University School of Medicine, Jinan, Shandong, P.R. China.
authors.forename: X M
authors.initials: XM
authors.lastname: Zhang
mesh_terms: D000544:Alzheimer Disease / Q000139:chemically induced / Q000188:drug therapy* / Q000523:psychology; D016229:Amyloid beta-Peptides; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal; D001923:Brain Chemistry / Q000187:drug effects; D005260:Female; D051550:I-kappa B Kinase / Q000378:metabolism; D007150:Immunohistochemistry; D007529:Isoflavones / Q000494:pharmacology*; D018782:Maze Learning / Q000187:drug effects; D008569:Memory Disorders / Q000188:drug therapy* / Q000523:psychology; D017628:Microglia / Q000187:drug effects; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D010446:Peptide Fragments; D051745:Protein Kinase C-delta / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016229:Amyloid beta-Peptides; D000894:Anti-Inflammatory Agents, Non-Steroidal; D007529:Isoflavones; D010446:Peptide Fragments; C521069:acetylpuerarin; C075222:amyloid beta-protein (1-42); D052247:Nitric Oxide Synthase Type II; D051550:I-kappa B Kinase; D051745:Protein Kinase C-delta