title: Ovocalyxin-36 is a pattern recognition protein in chicken eggshell membranes.
abstract: The avian eggshell membranes are essential elements in the fabrication of the calcified shell as a defense against bacterial penetration. Ovocalyxin-36 (OCX-36) is an abundant avian eggshell membrane protein, which shares protein sequence homology to bactericidal permeability-increasing protein (BPI), lipopolysaccharide-binding protein (LBP) and palate, lung and nasal epithelium clone (PLUNC) proteins. We have developed an efficient method to extract OCX-36 from chicken eggshell membranes for purification with cation and anion exchange chromatographies. Purified OCX-36 protein exhibited lipopolysaccharide (LPS) binding activity and bound lipopolysaccharide (LPS) from Escherichia coli O111:B4 in a dose-dependent manner. OCX-36 showed inhibitory activity against growth of Staphylococcus aureus ATCC 6538. OCX-36 single nucleotide polymorphisms (SNPs) were verified at cDNA 211 position and the corresponding proteins proline-71 (Pro-71) or serine-71 (Ser-71) were purified from eggs collected from genotyped hens. A significant difference between Pro-71 and Ser-71 OCX-36 for S. aureus lipoteichoic acid (LTA) binding activity was detected. The current study is a starting point to understand the innate immune role that OCX-36 may play in protection against bacterial invasion of both embryonated eggs (relevant to avian reproductive success) and unfertilized table eggs (relevant to food safety).
authors.affiliation: Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada.
authors.forename: Cristianne M M
authors.initials: CM
authors.lastname: Cordeiro
authors.affiliation: Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada.
authors.forename: Hamed
authors.initials: H
authors.lastname: Esmaili
authors.affiliation: ISA North America, Division of Hendrix Genetics, Kitchener, Ontario, Canada.
authors.forename: George
authors.initials: G
authors.lastname: Ansah
authors.affiliation: Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada.
authors.forename: Maxwell T
authors.initials: MT
authors.lastname: Hincke
mesh_terms: D000209:Acute-Phase Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000378:metabolism*; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001419:Bacteria / Q000472:pathogenicity; D001424:Bacterial Infections / Q000378:metabolism / Q000382:microbiology / Q000517:prevention & control*; D001798:Blood Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D002645:Chickens; D002853:Chromatography, Liquid; D004527:Egg Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D008070:Lipopolysaccharides / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D053719:Tandem Mass Spectrometry; D013682:Teichoic Acids / Q000378:metabolism; D000099231:Lipopolysaccharide-Binding Protein
chemical_list: D000209:Acute-Phase Proteins; D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D002352:Carrier Proteins; D004527:Egg Proteins; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D013682:Teichoic Acids; D000099231:Lipopolysaccharide-Binding Protein; C021623:bactericidal permeability increasing protein; C009900:lipoteichoic acid