title: [Simultaneous determination of principal components and related substances of raw material drug of ammonium glycyrrhizinate by reversed-phase high performance liquid chromatography].
abstract: An analytical method for the simultaneous determination of 18alpha-glycyrrhizic acid, 18beta-glycyrrhizinic acid, related substances A and B and drug quality standard by reversed-phase high performance liquid chromatography (RP-HPLC) was established. The assay was carried out on a Durashell-C18 column (250 mm x 4.6 mm, 5 microm) with 10 mmol/L ammonium perchlorate (the pH value was adjusted to 8.20 with ammonia)-methanol (48:52, v/v) as mobile phase at a flow rate of 0.80 mL/min, and the detection wavelength was set at 254 nm. The column temperature was 50 degrees C and the injection volume was 10 microL. Under the separation conditions, the calibration curves of the analytes showed good linearities within the mass concentrations of 0.50 -100 mg/L (r > 0.999 9). The detection limits for 18alpha-glycyrrhizic acid, 18beta-glycyrrhizinic acid, related substances A and B were 0.15, 0.10, 0.10, 0.15 mg/L, respectively. The average recoveries were between 97.32% and 99.33% (n = 3) with the relative standard deviations (RSDs) between 0.05% and 1.06%. The method is sensitive, reproducible, and the results are accurate and reliable. The method can be used for the determination of principal components and related substances of ammonium glycyrrhizinate for the quality control of raw material drug of ammonium glycyrrhizinate.
authors.affiliation: Medical Experimental Center, Hebei University, Baoding 071000, China. zhaoyany606@tom.com
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Medical Experimental Center, Hebei University, Baoding 071000, China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Liu
authors.affiliation: Medical Experimental Center, Hebei University, Baoding 071000, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Han
authors.affiliation: Medical Experimental Center, Hebei University, Baoding 071000, China.
authors.forename: Yueqiu
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology, Hebei Province, Key Laboratory of Chemical Biology, Hebei Province, Baoding 071002, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology, Hebei Province, Key Laboratory of Chemical Biology, Hebei Province, Baoding 071002, China.
authors.forename: Minjian
authors.initials: M
authors.lastname: Shi
mesh_terms: D064751:Ammonium Compounds / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D019695:Glycyrrhizic Acid / Q000737:chemistry*
chemical_list: D064751:Ammonium Compounds; D019695:Glycyrrhizic Acid