title: An attenuated coxsackievirus b3 vector: a potential tool for viral tracking study and gene delivery.
abstract: Cardiomyocytes are quite resistant to gene transfer using standard techniques. We developed an expression vector carrying an attenuated but infectious and replicative coxsackievirus B3 (CVB3) genome, and unique ClaI-StuI cloning sites for an exogenous gene, whose product can be released from the nascent viral polyprotein by 2A(pro) cleavage. This vector was tested as an expression vehicle for green fluorescent protein (GFP). The vector transiently expressed GFP in cell cultures for at least ten passages and delivered functional GFP to the infected cardiomyocytes for at least 6 days. Moreover, the recombinant viruses showed virulence attenuation in vitro and in vivo. The findings suggest that the recombinant CVB3 vector could be a useful tool for viral tracking study and delivering exogenous proteins to cardiomyocytes.
authors.affiliation: Department of Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong, People's Republic of China ; Department of Endocrinology, The First Hospital of Yichang, Three Gorges University College of Medicine, Yichang, Hubei, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zeng
authors.affiliation: Department of Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong, People's Republic of China.
authors.forename: Xiao xuan
authors.initials: Xx
authors.lastname: Chen
authors.affiliation: Department of Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong, People's Republic of China.
authors.forename: Jian ping
authors.initials: Jp
authors.lastname: Dai
authors.affiliation: Department of Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong, People's Republic of China.
authors.forename: Xiang feng
authors.initials: Xf
authors.lastname: Zhao
authors.affiliation: Department of Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Xin
authors.affiliation: Department of Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Su
authors.affiliation: Department of Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong, People's Republic of China.
authors.forename: Ge fei
authors.initials: Gf
authors.lastname: Wang
authors.affiliation: Department of Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong, People's Republic of China.
authors.forename: Yin xia
authors.initials: Yx
authors.lastname: Yan
authors.affiliation: Department of Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong, People's Republic of China.
authors.forename: Jing hua
authors.initials: Jh
authors.lastname: Su
authors.affiliation: Department of Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong, People's Republic of China.
authors.forename: Yu xue
authors.initials: Yx
authors.lastname: Deng
authors.affiliation: Department of Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong, People's Republic of China.
authors.forename: Kang sheng
authors.initials: Ks
authors.lastname: Li
mesh_terms: D000818:Animals; D002460:Cell Line; D029822:Enterovirus B, Human / Q000235:genetics* / Q000472:pathogenicity; D004769:Enterovirus Infections / Q000473:pathology / Q000821:virology; D015870:Gene Expression; D023061:Gene Order; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D016679:Genome, Viral; D006801:Humans; D008297:Male; D051379:Mice; D032383:Myocytes, Cardiac / Q000378:metabolism; D014161:Transduction, Genetic; D019076:Transgenes; D014774:Virulence