title: Glottic regeneration with a tissue-engineering technique, using acellular extracellular matrix scaffold in a canine model.
abstract: Acellular extracellular matrix scaffold derived from porcine urinary bladder (UBM) is decellularized material that has shown success for constructive remodelling of various tissues and organs. The regenerative effects of UBM were reported for the tympanic membrane, oesophagus, trachea, larynx, pleura and pericardium in animal studies, with promising results. The aim of this study was to investigate the regenerative effects of UBM on hemilarynx, using a canine model. A left partial hemilaryngectomy was performed and the surgical defects were reconstructed by insertion of UBM scaffold. Although local infection was observed in one dog in 1 week after implantation of the scaffold, all dogs showed good re-epithelialization with minimum complication in 1 month. The effect of regeneration of the larynx was evaluated 6 months after the operation. The excised larynx experiments were performed to measure phonation threshold pressure (PTP), normalized mucosal wave amplitude (NMWA) and normalized glottal gap (NGG). The results of the measurements showed that PTP was normal or near normal in two cases and NMWA was within normal range in three cases, although there were individual variations. Histological examination was completed to evaluate structural changes in the scaffold with the appearance of the new cartilaginous structure. However, the regenerated vocal fold mucosa was mostly scarred. The UBM scaffold has shown to be biocompatible, biodegradable and useful for tissue regeneration of the hemilarynx, with possible restoration of function of the vocal fold. The vocal fold mucosa was scarred, which is the next challenge to be addressed. Copyright © 2014 John Wiley & Sons, Ltd.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Japan. m_kitamura@ent.kuhp.kyoto-u.ac.jp.
authors.forename: Morimasa
authors.initials: M
authors.lastname: Kitamura
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Hirano
authors.affiliation: Department of Otolaryngology, Regenerative Treatment for Tympanic Membrane, Foundation for Biomedical Research and Innovation, Kobe, Japan.|Department of Otolaryngology, Head and Neck Surgery, Kitano Hospital, Tazuke Kofukai Medical Research Institute, Osaka, Japan.
authors.forename: Shin-Ichi
authors.initials: SI
authors.lastname: Kanemaru
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Japan.
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Kitani
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ohno
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kojima
authors.affiliation: Department of Bioartificial Organs, Institute for Frontier Medical Sciences, Kyoto University, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Japan.
authors.forename: Juichi
authors.initials: J
authors.lastname: Ito
authors.affiliation: Department of Otolaryngology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
authors.forename: Clark A
authors.initials: CA
authors.lastname: Rosen
authors.affiliation: McGowan Institute for Regenerative Medicine, Department of Surgery, University of Pittsburgh, PA, USA.
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Gilbert
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D012038:Regeneration; D013552:Swine; D054457:Tissue Scaffolds / Q000737:chemistry*; D001743:Urinary Bladder / Q000737:chemistry*; D014827:Vocal Cords / Q000293:injuries / Q000502:physiology / Q000601:surgery
keywords: acellular extracellular matrix; glottic regeneration; laryngeal function; porcine urinary bladder; scaffold; tissue engineering