title: Cystic fibrosis transmembrane regulator (CFTR) in human trophoblast BeWo cells and its relation to cell migration.
abstract: BACKGROUND
  ENaC and CFTR are coexpressed in epithelia and have positive or negative functional interactions. In addition, ENaC and CFTR promote migration in placental trophoblastic cells and human airway cells, respectively. Here we tested the idea if CFTR is functionally expressed in BeWo cells, a trophoblastic cell line, and if it is involved in their migratory behavior.
  
  
  METHODS
  CFTR expression was studied in BeWo cells with RT-PCR, biotinylation and Western blot. Ion currents were analyzed with patch clamp, and cell migration with the wound healing method.
  
  
  RESULTS
  The mature CFTR 160-kDa band was present, and its localization at the surface membrane was confirmed. Forskolin (20 μM), an adenylate cyclase activator, was used for channel activation, and subsequently CFTR(inh)-172 (2 μM) for its inhibition. The conductances in the presence of CFTR(inh)-172 plus forskolin (16.0 ± 0.7 pS/pF and 32.6 ± 1.5 pS/pF) were significantly lower than in presence of only forskolin (29.7 ± 0.9 and 47.0 ± 2.0 pS/pF). The conductance of CFTR(inh)-172 inhibited currents was 14.9 ± 0.7 pS/pF with a linear I-V relationship illustrating the nonrectifying properties of the CFTR. Cell migration was measured and covered 11.2 ± 0.4, 24.0 ± 1.7 and 13.9 ± 1.0% of the wound when cells were cultivated under control, forskolin, and forskolin plus CFTR(inh)-172, respectively. Proliferation was not changed by any of the treatments.
  
  
  CONCLUSIONS
  Our results shows that BeWo cells functionally express the CFTR which plays a role in the wound healing increasing the cell migration process.
authors.affiliation: Laboratorio de Canales Iónicos, Instituto de Investigaciones Médicas A. Lanari, IDIM-CONICET, Universidad de Buenos Aires, C. de Malvinas 3150, 1427 Buenos Aires, Argentina. Electronic address: gabinemar@gmail.com.
authors.forename: G I
authors.initials: GI
authors.lastname: Marino
authors.affiliation: Laboratorio de Canales Iónicos, Instituto de Investigaciones Médicas A. Lanari, IDIM-CONICET, Universidad de Buenos Aires, C. de Malvinas 3150, 1427 Buenos Aires, Argentina.
authors.forename: B A
authors.initials: BA
authors.lastname: Kotsias
mesh_terms: D001565:Benzoates / Q000494:pharmacology; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D005576:Colforsin / Q000494:pharmacology; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000096:biosynthesis / Q000502:physiology*; D053503:Epithelial Sodium Channels / Q000187:drug effects / Q000502:physiology; D006801:Humans; D053778:Thiazolidines / Q000494:pharmacology; D014327:Trophoblasts / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology
chemical_list: C482900:3-((3-trifluoromethyl)phenyl)-5-((3-carboxyphenyl)methylene)-2-thioxo-4-thiazolidinone; D001565:Benzoates; D053503:Epithelial Sodium Channels; D053778:Thiazolidines; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D005576:Colforsin
keywords: BeWo cells; CFTR; CFTR(inh)-172; Forskolin; Ion currents; Wound healing