title: Light-regulated gene repositioning in Arabidopsis.
abstract: Plant genomes are extremely sensitive to, and can be developmentally reprogrammed by environmental light cues. Here using rolling-circle amplification of gene-specific circularizable oligonucleotides coupled with fluorescence in situ hybridization, we demonstrate that light triggers a rapid repositioning of the Arabidopsis light-inducible chlorophyll a/b-binding proteins (CAB) locus from the nuclear interior to the nuclear periphery during its transcriptional activation. CAB repositioning is mediated by the red/far-red photoreceptors phytochromes (PHYs) and is inhibited by repressors of PHY signalling, including COP1, DET1 and PIFs. CAB repositioning appears to be a separate regulatory step occurring before its full transcriptional activation. Moreover, the light-inducible loci RBCS, PC and GUN5 undergo similar repositioning behaviour during their transcriptional activation. Our study supports a light-dependent gene regulatory mechanism in which PHYs activate light-inducible loci by relocating them to the nuclear periphery; it also provides evidence for the biological importance of gene positioning in the plant kingdom.
authors.affiliation: Department of Biology, Duke University, Durham, North Carolina 27708, USA.
authors.forename: Chun-Miao
authors.initials: CM
authors.lastname: Feng
authors.affiliation: Department of Biology, Duke University, Durham, North Carolina 27708, USA.
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Department of Biology, Duke University, Durham, North Carolina 27708, USA.
authors.forename: Elise K
authors.initials: EK
authors.lastname: Van Buskirk
authors.affiliation: Department of Biology, Duke University, Durham, North Carolina 27708, USA.
authors.forename: Emily J
authors.initials: EJ
authors.lastname: Yang
authors.affiliation: Department of Biology, Duke University, Durham, North Carolina 27708, USA.
authors.forename: Meng
authors.initials: M
authors.lastname: Chen
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D061146:Chlorophyll Binding Proteins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D015321:Gene Rearrangement / Q000235:genetics*; D047908:Intracellular Signaling Peptides and Proteins; D008027:Light; D008190:Lyases / Q000235:genetics; D009687:Nuclear Proteins / Q000378:metabolism; D051942:Phytochrome A / Q000378:metabolism; D051939:Phytochrome B / Q000378:metabolism; D010970:Plastocyanin / Q000235:genetics; D015533:Transcriptional Activation; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D061146:Chlorophyll Binding Proteins; C088435:DET1 protein, Arabidopsis; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; C543876:PETE1 protein, Arabidopsis; C497973:PHYA protein, Arabidopsis; C497952:PHYB protein, Arabidopsis; D051942:Phytochrome A; D051939:Phytochrome B; D010970:Plastocyanin; C471983:AT2G32950 protein, Arabidopsis; D044767:Ubiquitin-Protein Ligases; D008190:Lyases; C561080:GUN5 protein, Arabidopsis