title: Striking similarity in the gene expression levels of individual Myc module members among ESCs, EpiSCs, and partial iPSCs.
abstract: Predominant transcriptional subnetworks called Core, Myc, and PRC modules have been shown to participate in preservation of the pluripotency and self-renewality of embryonic stem cells (ESCs). Epiblast stem cells (EpiSCs) are another cell type that possesses pluripotency and self-renewality. However, the roles of these modules in EpiSCs have not been systematically examined to date. Here, we compared the average expression levels of Core, Myc, and PRC module genes between ESCs and EpiSCs. EpiSCs showed substantially higher and lower expression levels of PRC and Core module genes, respectively, compared with those in ESCs, while Myc module members showed almost equivalent levels of average gene expression. Subsequent analyses revealed that the similarity in gene expression levels of the Myc module between these two cell types was not just overall, but striking similarities were evident even when comparing the expression of individual genes. We also observed equivalent levels of similarity in the expression of individual Myc module genes between induced pluripotent stem cells (iPSCs) and partial iPSCs that are an unwanted byproduct generated during iPSC induction. Moreover, our data demonstrate that partial iPSCs depend on a high level of c-Myc expression for their self-renewal properties.
authors.affiliation: Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical University, Hidaka, Saitama, Japan.
authors.forename: Masataka
authors.initials: M
authors.lastname: Hirasaki
authors.affiliation: Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical University, Hidaka, Saitama, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Hiraki-Kamon
authors.affiliation: Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical University, Hidaka, Saitama, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Kamon
authors.affiliation: Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical University, Hidaka, Saitama, Japan.
authors.forename: Ayumu
authors.initials: A
authors.lastname: Suzuki
authors.affiliation: Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical University, Hidaka, Saitama, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Katano
authors.affiliation: Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical University, Hidaka, Saitama, Japan.
authors.forename: Masazumi
authors.initials: M
authors.lastname: Nishimoto
authors.affiliation: Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical University, Hidaka, Saitama, Japan ; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, Kawaguchi, Saitama, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Okuda
mesh_terms: D000818:Animals; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D005855:Germ Layers / Q000166:cytology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: D016271:Proto-Oncogene Proteins c-myc; D014157:Transcription Factors; C111264:peroxisome-proliferator-activated receptor-gamma coactivator-1