title: Altered microRNA and gene expression in the follicular fluid of women with polycystic ovary syndrome.
abstract: OBJECTIVE
  To determine if microRNAs are differentially expressed in the follicular fluid of women with PCOS compared to fertile oocyte donors and identify associated altered gene expression.
  
  
  METHODS
  Women undergoing IVF who met Rotterdam criteria for PCOS or who were fertile oocyte donors were recruited from a private IVF center. Individual follicle fluid was collected at the time of oocyte retrieval. MicroRNA analysis was performed using microarray and validated using real-time PCR on additional samples. Potential gene targets were identified and their expression analyzed by real time PCR.
  
  
  RESULTS
  Microarray profiling of human follicular fluid revealed expression of 235 miRNAs, 29 were differentially expressed between the groups. Using PCR validation, 5 miRNAs (32, 34c, 135a, 18b, and 9) showed significantly increased expression in the PCOS group. Pathway analysis revealed genes involved in insulin regulation and inflammation. Three potential target genes were found to have significantly decreased expression in the PCOS group (interleukin 8, synaptogamin 1, and insulin receptor substrate 2).
  
  
  CONCLUSIONS
  MicroRNAs are differentially expressed in the follicular fluid of women with PCOS when compared to fertile oocyte donors. There is also altered expression of potential target genes associated with the PCOS phenotype.
authors.affiliation: Division of Reproductive Endocrinology and Infertility, University of Colorado, 3055 Roslyn Street, Ste 230, Denver, CO, 80238, USA, lauren.roth@ucdenver.edu.
authors.forename: Lauren W
authors.initials: LW
authors.lastname: Roth
authors.forename: Blair
authors.initials: B
authors.lastname: McCallie
authors.forename: Ruben
authors.initials: R
authors.lastname: Alvero
authors.forename: William B
authors.initials: WB
authors.lastname: Schoolcraft
authors.forename: Debra
authors.initials: D
authors.lastname: Minjarez
authors.forename: Mandy G
authors.initials: MG
authors.lastname: Katz-Jaffe
mesh_terms: D000328:Adult; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics*; D006107:Granulosa Cells / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009865:Oocytes / Q000378:metabolism; D011085:Polycystic Ovary Syndrome / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D035683:MicroRNAs