title: [New method for analyzing pharmacodynamic material basis of traditional Chinese medicines by using specific knockout technology with monoclonal antibodies].
abstract: Study on pharmacodynamic material basis of traditional Chinese medicines is one of the key issues for the modernization of traditional Chinese medicine. Having introduced the monoclonal antibody technology into the study on pharmacodynamic material basis of traditional Chinese medicines, the author prepared the immunoaffinity chromatography column by using monoclonal antibodies in active components of traditional Chinese medicines, so as to selectively knock out the component from herbs or traditional Chinese medicine compounds, while preserving all of the other components and keeping their amount and ratio unchanged. A comparative study on pharmacokinetics and pharmacodynamics was made to explicitly reveal the correlation between the component and the main purpose of traditional Chinese medicines and compounds. The analysis on pharmacodynamic material basis of traditional Chinese medicines by using specific knockout technology with monoclonal antibodies is a new method for study pharmacodynamic material basis in line with the characteristics of traditional Chinese medicines. Its results can not only help study material basis from a new perspective, but also help find the modern scientific significance in single herb or among compounds of traditional Chinese medicines.
authors.affiliation: The School of Preclinical Medicine, Beijing University of Chinese Medcine, Beijing 100029, China. zhaoyandr@gmail.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Center of Scientific Research, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Hui-Hua
authors.initials: HH
authors.lastname: Qu
authors.affiliation: The School of Preclinical Medicine, Beijing University of Chinese Medcine, Beijing 100029, China.
authors.forename: Qing-Guo
authors.initials: QG
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002460:Cell Line; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans
chemical_list: D000911:Antibodies, Monoclonal; D004365:Drugs, Chinese Herbal