title: [Detection of a clonal complex with Brucella abortus biovar 2 genotype as founder in B. abortus isolates from Argentina].
abstract: Brucella abortus is the causative agent of bovine brucellosis, a worldwide zoonosis. Up to date, eight biovars of B. abortus have been described. In Argentina, biovar 1 is the most frequently isolated. However, biovar 2, which is more pathogenic than biovar 1, is also found. Molecular methods for subtyping isolates are necessary for allowing epidemiological surveillance and control of eradication programs. Due to the genetic homogeneity of the genus Brucella, the development of molecular typing tools has been difficult. The publication of microorganism genomes facilitates the design of this approach. The aim of this work was to employ a Multiple Locus VNTR Analysis (MLVA) scheme for strains from Argentina isolated in our laboratory. From the 56 isolates analyzed, 47 different genotypic profiles were obtained. All the strains typed as biovar 2 showed the same profile. This scheme allowed assigning each isolate to the biovar it belongs to. All the genotypes were related using the goeBURST analysis and biovar 2 was proposed as founder.
authors.affiliation: Laboratorio de Brucella, Instituto de Biotecnología, CICVyA, INTA Castelar, Las Cabañas y Nicolás Repetto, Hurlingham (1686), Buenos Aires, Argentina. Electronic address: dhollender@cnia.inta.gov.ar.
authors.forename: Daiana
authors.initials: D
authors.lastname: Hollender
authors.affiliation: AER Lobos - INTA, EEA Pergamino, Centro Regional Buenos Aires Norte, Pergamino, Provincia de Buenos Aires, Argentina.
authors.forename: Sandra B
authors.initials: SB
authors.lastname: Conde
authors.affiliation: Falleció 7/12/11.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Salustio
authors.affiliation: Laboratorio de Brucelosis, Instituto de Patobiología, CICVyA, INTA Castelar, Las Cabañas y Nicolás Repetto, Hurlingham (1686), Buenos Aires, Argentina(1).
authors.forename: Luis E
authors.initials: LE
authors.lastname: Samartino
mesh_terms: D001118:Argentina; D002003:Brucella abortus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans
keywords: Brucella; Epidemiology; Epidemiología; MLVA; Molecular typing; Tipificación molecular