title: Development of a multiplex PCR assay to detect Edwardsiella tarda, Streptococcus parauberis, and Streptococcus iniae in olive flounder (Paralichthys olivaceus).
abstract: A multiplex PCR protocol was established to simultaneously detect major bacterial pathogens in olive flounder (Paralichthys olivaceus) including Edwardsiella (E.) tarda, Streptococcus (S.) parauberis, and S. iniae. The PCR assay was able to detect 0.01 ng of E. tarda, 0.1 ng of S. parauberis, and 1 ng of S. iniae genomic DNA. Furthermore, this technique was found to have high specificity when tested with related bacterial species. This method represents a cheaper, faster, and reliable alternative for identifying major bacterial pathogens in olive flounder, the most important farmed fish in Korea.
authors.affiliation: Aquatic Biotechnology Center, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Seong Bin
authors.initials: SB
authors.lastname: Park
authors.forename: Kyoung
authors.initials: K
authors.lastname: Kwon
authors.forename: In Seok
authors.initials: IS
authors.lastname: Cha
authors.forename: Ho Bin
authors.initials: HB
authors.lastname: Jang
authors.forename: Seong Won
authors.initials: SW
authors.lastname: Nho
authors.forename: Fernand F
authors.initials: FF
authors.lastname: Fagutao
authors.forename: Young Kyu
authors.initials: YK
authors.lastname: Kim
authors.forename: Jong Earn
authors.initials: JE
authors.lastname: Yu
authors.forename: Tae Sung
authors.initials: TS
authors.lastname: Jung
mesh_terms: D000818:Animals; D020609:Edwardsiella tarda / Q000235:genetics / Q000302:isolation & purification*; D004756:Enterobacteriaceae Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D005393:Fish Diseases / Q000175:diagnosis* / Q000382:microbiology; D005398:Fisheries / Q000379:methods*; D005412:Flatfishes; D060885:Multiplex Polymerase Chain Reaction / Q000191:economics / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013290:Streptococcal Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D013291:Streptococcus / Q000235:genetics / Q000302:isolation & purification*
keywords: Edwardsiella tarda; Streptococcus iniae; Streptococcus parauberis; multiplex PCR; olive flounder