title: Addition of magnesium chloride to enhance mono-dispersity of a coiled-coil recombinant mouse macrophage protein.
abstract: X-ray crystallography for the determination of three-dimensional structures of protein macromolecules represents an important tool in function assignment of uncharacterized proteins. However, crystallisation is often difficult to achieve. A protein sample fully characterized in terms of dispersity may increase the likelihood of successful crystallisation by improving the predictability of the crystallisation process. To maximize the probability of crystallisation of a novel mouse macrophage protein (rMMP), target molecule was characterized and refined to improve monodispersity. Addition of MgCl2 at low concentrations resolves the rMMP into a monodisperse solution, and finally successful crystallization of rMMP was achieved. The effect of MgCl2 was studied using gel filtration chromatography and dynamic light scattering.
authors.affiliation: Fermentation and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, India.
authors.forename: Parveen
authors.initials: P
authors.lastname: Pahuja
authors.forename: Alagiri
authors.initials: A
authors.lastname: Srinivasan
authors.forename: Munish
authors.initials: M
authors.lastname: Puri
mesh_terms: D000818:Animals; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray / Q000379:methods; D008264:Macrophages / Q000737:chemistry*; D015636:Magnesium Chloride / Q000737:chemistry*; D051379:Mice; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry*
chemical_list: D011994:Recombinant Proteins; D015636:Magnesium Chloride