title: Domain of dentine sialoprotein mediates proliferation and differentiation of human periodontal ligament stem cells.
abstract: Classic embryological studies have documented the inductive role of root dentin on adjacent periodontal ligament differentiation.  The biochemical composition of root dentin includes collagens and cleavage products of dentin sialophosphoprotein (DSPP), such as dentin sialoprotein (DSP).  The high abundance of DSP in root dentin prompted us to ask the question whether DSP or peptides derived thereof would serve as potent biological matrix components to induce periodontal progenitors to further differentiate into periodontal ligament cells. Here, we test the hypothesis that domain of DSP influences cell fate. In situ hybridization and immunohistochemical analyses showed that the COOH-terminal DSP domain is expressed in mouse periodontium at various stages of root development. The recombinant COOH-terminal DSP fragment (rC-DSP) enhanced attachment and migration of human periodontal ligament stem cells (PDLSC), human primary PDL cells without cell toxicity. rC-DSP induced PDLSC cell proliferation as well as differentiation and mineralization of PDLSC and PDL cells by formation of mineralized tissue and ALPase activity. Effect of rC-DSP on cell proliferation and differentiation was to promote gene expression of tooth/bone-relate markers, transcription factors and growth factors. The results for the first time showed that rC-DSP may be one of the components of cell niche for stimulating stem/progenitor cell proliferation and differentiation and a natural scaffold for periodontal regeneration application.
authors.affiliation: Department of Developmental Dentistry, Dental School, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America ; Department of Orthodontics, YüzüncüYıl University, Faculty of Dentistry, Kampus-Van, Turkey.
authors.forename: Alkan
authors.initials: A
authors.lastname: Ozer
authors.affiliation: State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Yuan
authors.affiliation: State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: Guobin
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Developmental Dentistry, Dental School, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Developmental Dentistry, Dental School, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America.
authors.forename: Wentong
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Developmental Dentistry, Dental School, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Developmental Dentistry, Dental School, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America.
authors.forename: Feng
authors.initials: F
authors.lastname: Guo
authors.affiliation: Department of Developmental Dentistry, Dental School, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Department of Developmental Dentistry, Dental School, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America.
authors.forename: Lisa
authors.initials: L
authors.lastname: Shoff
authors.affiliation: State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Periodontics & Dental Hygiene, School of Dentistry, The University of Texas Health Science Center at Houston, Houston, Texas, United States of America.
authors.forename: Isabel C
authors.initials: IC
authors.lastname: Gay
authors.affiliation: Department of Developmental Dentistry, Dental School, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Donly
authors.affiliation: Department of Oral/Maxillofacial Surgery, University of Alabama, Birmingham, Alabama, United States of America.
authors.forename: Mary
authors.initials: M
authors.lastname: MacDougall
authors.affiliation: Department of Developmental Dentistry, Dental School, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America.
authors.forename: Shuo
authors.initials: S
authors.lastname: Chen
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D003804:Dentin / Q000378:metabolism* / Q000648:ultrastructure; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010513:Periodontal Ligament / Q000166:cytology*; D010519:Periodontium / Q000378:metabolism; D017434:Protein Structure, Tertiary; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000378:metabolism; D012795:Sialoglycoproteins / Q000378:metabolism*; D013234:Stem Cells / Q000502:physiology*; D014092:Tooth Root / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D012795:Sialoglycoproteins