title: ERK1/2 mediates lung adenocarcinoma cell proliferation and autophagy induced by apelin-13.
abstract: The aim of this study was to investigate the role of apelin in the cell proliferation and autophagy of lung adenocarcinoma. The over-expression of APJ in lung adenocarcinoma was detected by immunohistochemistry, while plasma apelin level in lung cancer patients was measured by enzyme-linked immunosorbent assay. Our findings revealed that apelin-13 significantly increased the phosphorylation of ERK1/2, the expression of cyclin D1, microtubule-associated protein 1 light chain 3A/B (LC3A/B), and beclin1, and confirmed that apelin-13 promoted A549 cell proliferation and induced A549 cell autophagy via ERK1/2 signaling. Moreover, there are pores on the surface of human lung adenocarcinoma cell line A549 and apelin-13 causes cell surface smooth and glossy as observed under atomic force microscopy. These results suggested that ERK1/2 signaling pathway mediates apelin-13-induced lung adenocarcinoma cell proliferation and autophagy. Under our experimental condition, autophagy associated with 3-methyladenine was not involved in cell proliferation.
authors.affiliation: Learning Key Laboratory for Pharmaco-proteomics, Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421001, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Tao
authors.initials: T
authors.lastname: Su
authors.forename: Deguan
authors.initials: D
authors.lastname: Lv
authors.forename: Feng
authors.initials: F
authors.lastname: Xie
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Jiangang
authors.initials: J
authors.lastname: Cao
authors.forename: Irshad Ali
authors.initials: IA
authors.lastname: Sheikh
authors.forename: Xuping
authors.initials: X
authors.lastname: Qin
authors.forename: Lanfang
authors.initials: L
authors.lastname: Li
authors.forename: Linxi
authors.initials: L
authors.lastname: Chen
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology / Q000503:physiopathology*; D000077192:Adenocarcinoma of Lung; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis; D001343:Autophagy / Q000187:drug effects*; D000071186:Beclin-1; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D019938:Cyclin D1; D048049:Extracellular Signal-Regulated MAP Kinases / Q000502:physiology*; D005419:Flavonoids / Q000494:pharmacology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000097:blood / Q000502:physiology; D008175:Lung Neoplasms / Q000473:pathology / Q000503:physiopathology*; D008565:Membrane Proteins / Q000096:biosynthesis; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis; D010766:Phosphorylation / Q000187:drug effects; D015398:Signal Transduction
chemical_list: D051017:Apoptosis Regulatory Proteins; C491997:BECN1 protein, human; D000071186:Beclin-1; D005419:Flavonoids; D036341:Intercellular Signaling Peptides and Proteins; C477012:MAP1LC3A protein, human; C502070:MAP1LC3B protein, human; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; C495199:apelin-13 peptide; D019938:Cyclin D1; D048049:Extracellular Signal-Regulated MAP Kinases; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one
keywords: APJ; ERK1/2; apelin; autophagy; lung adenocarcinoma; proliferation