title: Study on the interaction between 4-thio-5-methyluridine and human serum albumin by spectroscopy and molecular modeling.
abstract: The interaction between 4-thio-5-methyluridine and human serum albumin (HSA) under simulative physiological conditions has been studied by the methods of fluorescence, UV-VIS absorbance and circular dichroism (CD) spectroscopy. The results show that 4-thio-5-methyluridine has a static fluorescence quenching on human serum albumin (HSA). The main forces of both interactions have typical static interaction from thermodynamic data to the determination. The experimental result was in correspondence with molecular modeling theory.
authors.affiliation: College of Environment and Chemical Engineering, Dalian University, Dalian, Liaoning 116622, China. Electronic address: xiaohui99@hotmail.co.uk.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Environment and Chemical Engineering, Dalian University, Dalian, Liaoning 116622, China.
authors.forename: Hongxiu
authors.initials: H
authors.lastname: Zhai
authors.affiliation: College of Environment and Chemical Engineering, Dalian University, Dalian, Liaoning 116622, China.
authors.forename: Ruiqi
authors.initials: R
authors.lastname: Gao
authors.affiliation: MOE Key laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, Guangdong 510631, China.
authors.forename: Juling
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Environment and Chemical Engineering, Dalian University, Dalian, Liaoning 116622, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Environment and Chemical Engineering, Dalian University, Dalian, Liaoning 116622, China.
authors.forename: Xuefang
authors.initials: X
authors.lastname: Zheng
mesh_terms: D002942:Circular Dichroism; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D012709:Serum Albumin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013891:Thiouridine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D014529:Uridine / Q000737:chemistry / Q000378:metabolism*
chemical_list: D012709:Serum Albumin; D013891:Thiouridine; D014529:Uridine
keywords: 4-Thio-5-methyluridine; Fluorescence spectroscopy; Human serum albumin (HSA); Molecular modeling; Nucleoside