title: Stem Cell Niche is Partially Lost during Follicular Plucking: A Preliminary Pilot Study.
abstract: BACKGROUND
  Clinical hair transplant studies have revealed that follicular unit extraction (FUE) is superior in terms of stable hair growth in comparison to follicular plucking (FP). Various reasons have been cited for this clinical outcome. FUE and FP are employed to obtain the hair follicle units for hair transplant and recently for cell based therapies in vitiligo. However, there is no scientific data available on the comparison of stem cell fraction in the cell suspension obtained by FUE and FP. Therefore, we undertook this study to compare the percentage of stem cells in the hair follicle obtained by FUE and FP.
  
  
  OBJECTIVE
  The purpose of the following study is to evaluate the quantitative stem cell pool in the hair follicle obtained by FUE and FP.
  
  
  METHODS
  A total of 3 human subjects were enrolled with age groups of 17-25 years. Both methods of tissue harvest: FUE and FP; were employed on each subject. There was no vitiligo lesion on the scalp in any of the patients. Hair follicles were incubated with trypsin-EDTA solution at 37°C for 90 min to separate outer root sheath cells. The cell suspension was passed through a 70 μm cell strainer; filtrate was centrifuged to obtain the cell pellet. Cells were labeled with cluster of differentiation (CD200) antibody and acquired with flowcytometry.
  
  
  RESULTS
  The mean percentage of CD200 positive cells in FUE and FP method come out to be 8.43 and 1.63 respectively (P = 0.0152).
  
  
  CONCLUSIONS
  FUE is a better method of the hair follicle harvesting for cell based applications as the stem cell fraction is significantly higher in comparison to FP.
authors.affiliation: Department of AIIMS, Stem Cell Facility, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Anil
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Dermatology and Venereology, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Somesh
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Department of AIIMS, Stem Cell Facility, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Sujata
authors.initials: S
authors.lastname: Mohanty
authors.affiliation: Department of Cardiology, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Balram
authors.initials: B
authors.lastname: Bhargava
authors.affiliation: Department of Cardio-Thoracic Vascular Surgery, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Balram
authors.initials: B
authors.lastname: Airan
keywords: Cluster of differentiation; follicular plucking; follicular unit extraction; stem cells