title: [Spectrum-effect correlation analysis of traditional Tibetan medicine "Morina nepalensis" on nitric oxide production inhibition].
abstract: OBJECTIVE
  To establish an HPLC-ELSD fingerprint of the whole herbs of Morina nepalensis and perform the correlation analysis of chemical components of the herb and nitric oxide (NO) production inhibition.
  
  
  METHODS
  HPLC-ELSD assay was performed to evaluate 10 batches of M. nepalensis herbs. The chromatographic conditions were as following: Eclipse XDB C18 column (4.6 mm x 150 mm, 5 microm), water (A) and acetonitrile (B) as a gradient mobile phases, flow rate 1.0 mL x min(-1), and column temperature at 35 degress C. Evaporative light-detection conditions: atomization temperature at 104 degrees C, the flow rate of N2 2.8 L x min(-1) and 10 microL sample injection. Chromatographic fingerprint was developed, and the inhibition activity of production of NO in lipopolysaccharide-induced macrophages was also analyzed. The similarity and correlation analysis between the HPLC-ELSD fingerprints and NO production inhibition were carried out by PLS method.
  
  
  RESULTS
  The common mode for M. nepalensis herb fingerprint was established, including 15 common characteristic peaks. Among them, 7 peaks were positively correlated with the NO production inhibition. According to the assessment on the similarity of 10 batches of samples, a similarity of over 0.90 were shown in HPLC-ELSD fingerprint and all samples were separated into two groups.
  
  
  CONCLUSIONS
  This method can be used to assess the quality of M. nepalensis, which provides a reliable method for scientific assessment and quality control.
authors.affiliation: State Key Laboratory for Quality Research of Chinese Medicines, Macau University of Science and Technology, Macau, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Luo
authors.affiliation: Ethnic Pharmaceutical Institute, Southwest University for Nationalities, Chengdu 610041, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Ethnic Pharmaceutical Institute, Southwest University for Nationalities, Chengdu 610041, China.
authors.forename: Lu-Yang
authors.initials: LY
authors.lastname: Lv
authors.affiliation: Ethnic Pharmaceutical Institute, Southwest University for Nationalities, Chengdu 610041, China.
authors.forename: Zhi-Feng
authors.initials: ZF
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000032:analysis / Q000494:pharmacology*; D027922:Caprifoliaceae / Q000737:chemistry*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D038861:Medicine, Tibetan Traditional; D051379:Mice; D009569:Nitric Oxide / Q000276:immunology*
chemical_list: D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D009569:Nitric Oxide