title: Diversity of endophytic bacteria in Malaysian plants as revealed by 16S rRNA encoding gene sequence based method of bacterial identification.
abstract: Bacterial endophytes do have several potential applications in pharmacy, medicine and agricultural biotech industry. The main objective of this study was to understand types of bacterial endophytes associated with dicotyledonous (dicot) and monocotyledonous (monocot) plant species. Isolation of the endophytic bacteria was performed using surface-sterilized various tissue samples, and identification of the endophytic bacterial isolates (EBIs) was completed using 16S rRNA encoding gene sequence similarity based method. In total, 996 EBIs were isolated and identified from 1055 samples of 31 monocot and 65 dicot plant species from Peninsular Malaysia. The 996 EBIs represented 71 different types of bacterial species. Twelve (12) out of 71 species are reported as endophytes for the first time. We conclude that diverse types of bacterial endophytes are associated with dicot and monocot plants, and could be useful in pharmacy, medicine and agricultural biotechnology for various potential applications.
authors.affiliation: Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Bedong 08100, Kedah, Malaysia.
authors.forename: Chye Ying
authors.initials: CY
authors.lastname: Loh
authors.affiliation: Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Bedong 08100, Kedah, Malaysia.
authors.forename: Yin Yin
authors.initials: YY
authors.lastname: Tan
authors.affiliation: Atta-ur-Rahman Research Institute of Natural Products for Drug Discovery (RiND) and Faculty of Pharmacy, MARA University of Technology (UiTM), Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia.
authors.forename: Rahim
authors.initials: R
authors.lastname: Rohani
authors.affiliation: Atta-ur-Rahman Research Institute of Natural Products for Drug Discovery (RiND) and Faculty of Pharmacy, MARA University of Technology (UiTM), Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia.
authors.forename: Jean-Frédéric F
authors.initials: JF
authors.lastname: Weber
authors.affiliation: Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Bedong 08100, Kedah, Malaysia.
authors.forename: Subhash Janardhan
authors.initials: SJ
authors.lastname: Bhore
keywords: Bioprospecting; Dicots; Endophytes; Monocots; Natural compounds