title: Determining cell-of-origin subtypes of diffuse large B-cell lymphoma using gene expression in formalin-fixed paraffin-embedded tissue.
abstract: The assignment of diffuse large B-cell lymphoma into cell-of-origin (COO) groups is becoming increasingly important with the emergence of novel therapies that have selective biological activity in germinal center B cell-like or activated B cell-like groups. The Lymphoma/Leukemia Molecular Profiling Project's Lymph2Cx assay is a parsimonious digital gene expression (NanoString)-based test for COO assignment in formalin-fixed paraffin-embedded tissue (FFPET). The 20-gene assay was trained using 51 FFPET biopsies; the locked assay was then validated using an independent cohort of 68 FFPET biopsies. Comparisons were made with COO assignment using the original COO model on matched frozen tissue. In the validation cohort, the assay was accurate, with only 1 case with definitive COO being incorrectly assigned, and robust, with >95% concordance of COO assignment between 2 independent laboratories. These qualities, along with the rapid turnaround time, make Lymph2Cx attractive for implementation in clinical trials and, ultimately, patient management.
authors.affiliation: Centre for Lymphoid Cancer, British Columbia Cancer Agency, Vancouver, BC, Canada;
authors.forename: David W
authors.initials: DW
authors.lastname: Scott
authors.forename: George W
authors.initials: GW
authors.lastname: Wright
authors.forename: P Mickey
authors.initials: PM
authors.lastname: Williams
authors.forename: Chih-Jian
authors.initials: CJ
authors.lastname: Lih
authors.forename: William
authors.initials: W
authors.lastname: Walsh
authors.forename: Elaine S
authors.initials: ES
authors.lastname: Jaffe
authors.forename: Andreas
authors.initials: A
authors.lastname: Rosenwald
authors.forename: Elias
authors.initials: E
authors.lastname: Campo
authors.forename: Wing C
authors.initials: WC
authors.lastname: Chan
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Connors
authors.forename: Erlend B
authors.initials: EB
authors.lastname: Smeland
authors.forename: Anja
authors.initials: A
authors.lastname: Mottok
authors.forename: Rita M
authors.initials: RM
authors.lastname: Braziel
authors.forename: German
authors.initials: G
authors.lastname: Ott
authors.forename: Jan
authors.initials: J
authors.lastname: Delabie
authors.forename: Raymond R
authors.initials: RR
authors.lastname: Tubbs
authors.forename: James R
authors.initials: JR
authors.lastname: Cook
authors.forename: Dennis D
authors.initials: DD
authors.lastname: Weisenburger
authors.forename: Timothy C
authors.initials: TC
authors.lastname: Greiner
authors.forename: Betty J
authors.initials: BJ
authors.lastname: Glinsmann-Gibson
authors.forename: Kai
authors.initials: K
authors.lastname: Fu
authors.forename: Louis M
authors.initials: LM
authors.lastname: Staudt
authors.forename: Randy D
authors.initials: RD
authors.lastname: Gascoyne
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Rimsza
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D019070:Cell Lineage / Q000235:genetics*; D005260:Female; D005404:Fixatives; D005557:Formaldehyde; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D016612:Paraffin Embedding; D014015:Tissue Banks; D059467:Transcriptome; D055815:Young Adult
chemical_list: D005404:Fixatives; D005557:Formaldehyde