title: Expression profiling of cervical cancers in Indian women at different stages to identify gene signatures during progression of the disease.
abstract: Cervical cancer is the second most common cancer among women worldwide, with developing countries accounting for >80% of the disease burden. Although in the West, active screening has been instrumental in reducing the incidence of cervical cancer, disease management is hampered due to lack of biomarkers for disease progression and defined therapeutic targets. Here we carried out gene expression profiling of 29 cervical cancer tissues from Indian women, spanning International Federation of Gynaecology and Obstetrics (FIGO) stages of the disease from early lesion (IA and IIA) to progressive stages (IIB and IIIA-B), and identified distinct gene expression signatures. Overall, metabolic pathways, pathways in cancer and signaling pathways were found to be significantly upregulated, while focal adhesion, cytokine-cytokine receptor interaction and WNT signaling were downregulated. Additionally, we identified candidate biomarkers of disease progression such as SPP1, proliferating cell nuclear antigen (PCNA), STK17A, and DUSP1 among others that were validated by quantitative real-time polymerase chain reaction (qRT-PCR) in the samples used for microarray studies as well in an independent set of 34 additional samples. Integrative analysis of our results with other cervical cancer profiling studies could facilitate the development of multiplex diagnostic markers of cervical cancer progression.
authors.affiliation: Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, Navi Mumbai, Maharashtra, India.
authors.forename: Asha
authors.initials: A
authors.lastname: Thomas
authors.forename: Umesh
authors.initials: U
authors.lastname: Mahantshetty
authors.forename: Sadhana
authors.initials: S
authors.lastname: Kannan
authors.forename: Kedar
authors.initials: K
authors.lastname: Deodhar
authors.forename: Shyam K
authors.initials: SK
authors.lastname: Shrivastava
authors.forename: Chandan
authors.initials: C
authors.lastname: Kumar-Sinha
authors.forename: Rita
authors.initials: R
authors.lastname: Mulherkar
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D018450:Disease Progression; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007194:India; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger
keywords: Biomarker; cervical cancer; expression profiling; microarray; real-time PCR