title: A facile approach toward protein-resistant biointerfaces based on photodefinable poly-p-xylylene coating.
abstract: A facile and versatile tool is reported that uses a photodefinable polymer, poly(4-benzoyl-p-xylylene-co-p-xylylene) to immobilize antifouling materials, such as poly(ethylene glycol), poly(ethylene glycol) methyl ether methacrylate, dextran, and ethanolamine. This immobilization process requires the polymer's photoactivated carbonyl groups, which can facilitate light-induced molecular crosslinking and can rapidly react via insertion into CH or NH bonds upon photo-illumination at 365 nm. Importantly, the process does not require additional functional groups on the antifouling materials. The immobilized fouling materials were characterized using X-ray photoelectron spectroscopy (XPS) and infrared reflection absorption spectroscopy (IRRAS), and the resulting antifouling properties were examined through protein adsorption studies on fibrinogen and bovine serum albumin at surfaces that were spatially modified using a photomask during the photochemical process. In addition, the adsorbed fibrinogen was quantitatively analyzed using a quartz crystal microbalance (QCM), and the adsorption values were reduced to 32.8 ± 4.9 ng cm(-2), 5.5 ± 3.9 ng cm(-2), 21.4 ± 4.5 ng cm(-2), and 16.9 ± 3.4 ng cm(-2) for poly(ethylene glycol) (PEG), poly(ethylene glycol) methyl ether methacrylate (PEGMA), dextran, and ethanolamine, respectively. Finally, this antifouling modification technology was demonstrated on an unconventional substrate for a stent that was modified by PEGMA at selected areas using a microscopic patterning technique during photoimmobilization. Low levels of fibrinogen and BSA adsorption were also observed at the areas where PEGMA was attached.
authors.affiliation: Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Chiao-Tzu
authors.initials: CT
authors.lastname: Su
authors.affiliation: Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Ruei-Hung
authors.initials: RH
authors.lastname: Yuan
authors.affiliation: Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Yung-Chih
authors.initials: YC
authors.lastname: Chen
authors.affiliation: Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Ting-Ju
authors.initials: TJ
authors.lastname: Lin
authors.affiliation: Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Hsiu-Wen
authors.initials: HW
authors.lastname: Chien
authors.affiliation: Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Chih-Chen
authors.initials: CC
authors.lastname: Hsieh
authors.affiliation: Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Wei-Bor
authors.initials: WB
authors.lastname: Tsai
authors.affiliation: Department of Orthopedics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei 10018, Taiwan. Electronic address: mike920@gmail.com.
authors.forename: Chih-Hao
authors.initials: CH
authors.lastname: Chang
authors.affiliation: Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan. Electronic address: hsychen@ntu.edu.tw.
authors.forename: Hsien-Yeh
authors.initials: HY
authors.lastname: Chen
mesh_terms: D000327:Adsorption; D000818:Animals; D057219:Biofouling; D002417:Cattle; D005340:Fibrinogen / Q000737:chemistry*; D055668:Photochemical Processes; D011108:Polymers / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D011108:Polymers; D012710:Serum Albumin, Bovine; D005340:Fibrinogen
keywords: Antifouling; Biointerface; CVD polymerization; Photopatterning; Protein adsorptions; Surface modifications