title: Mycobacterium tuberculosis DNA detection using surface plasmon resonance modulated by telecommunication wavelength.
abstract: A surface plasmon resonance sensor for Mycobacterium tuberculosis (MTB) deoxyribonucleic acid (DNA) is developed using repeatable telecommunication wavelength modulation based on optical fiber communications laser wavelength and stability. MTB DNA concentrations of 1 μg/mL and 10 μg/mL were successfully demonstrated to have the same spectral half-width in the dip for optimum coupling. The sensitivity was shown to be -0.087 dB/(μg/mL) at all applied telecommunication wavelengths and the highest sensitivity achieved was 115 ng/mL without thiolated DNA immobilization onto a gold plate, which is better than the sensor limit of 400 ng/mL possible with commercial biosensor equipment.
authors.affiliation: Department of Electronic Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Rd., Taipei 10607, Taiwan. shsu@mail.ntust.edu.tw.
authors.forename: Shih-Hsiang
authors.initials: SH
authors.lastname: Hsu
authors.affiliation: Department of Electronic Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Rd., Taipei 10607, Taiwan. a.j.lin26@gmail.com.
authors.forename: Yan-Yu
authors.initials: YY
authors.lastname: Lin
authors.affiliation: Department of Electronic Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Rd., Taipei 10607, Taiwan. m10002303@mail.ntust.edu.tw.
authors.forename: Shao-Hsi
authors.initials: SH
authors.lastname: Lu
authors.affiliation: Department of Electronic Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Rd., Taipei 10607, Taiwan. m9209001@gmail.com.
authors.forename: I-Fang
authors.initials: IF
authors.lastname: Tsai
authors.affiliation: Department of Electronic Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Rd., Taipei 10607, Taiwan. ytlhl@ms2.mmh.org.tw.
authors.forename: Yen-Ta
authors.initials: YT
authors.lastname: Lu
authors.affiliation: Department of Electronic Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Rd., Taipei 10607, Taiwan. PhotonicsNTUST@gmail.com.
authors.forename: Hsin-Tsung
authors.initials: HT
authors.lastname: Ho
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis*; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D020349:Surface Plasmon Resonance / Q000379:methods*; D013685:Telecommunications
chemical_list: D004269:DNA, Bacterial