title: Among the three striatin family members, SG2NA was first to arise during evolution.
abstract: Striatin, SG2NA, and zinedin constitute a three-member subfamily of WD-40 repeat proteins. They are found only in metazoans and are likely to have scaffolding functions. Apart from WD-40 repeats, they also have a caveolin-binding motif, a coiled-coil structure, and a calmodulin-binding domain. This paper focuses on the analysis of their evolution as a paradigm of understanding the metazoan scaffolds. Each member of the family forms distinct phylogenetic clusters, wherein striatins, SG2NAs, and zinedins have 13, 10, and 9 conserved motifs, respectively. Furthermore, two of those motifs each in striatin and in zinedin and three in SG2NA are exclusive for the respective subfamily. Of those exclusive motifs for SG2NA, two encompass the caveolin-binding and coiled-coiled domains. Collectively, they show the presence of 11 conserved motifs, suggestive of convergence of individual motifs and creation of patterns. A prokaryotic WD-40 repeat motif pCM-I was found only in the corresponding domain of SG2NA but not in other family members. It is thus hypothesized that striatin family members have evolved from bacteria, and SG2NA was the first member to arise.
authors.affiliation: Lab No 220, School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067.
authors.forename: Goutam Kumar
authors.initials: GK
authors.lastname: Tanti
authors.affiliation: Lab No 220, School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067.
authors.forename: Nandini
authors.initials: N
authors.lastname: Singarapu
authors.affiliation: Lab No 220, School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067.
authors.forename: Rohini
authors.initials: R
authors.lastname: Muthuswami
authors.affiliation: Lab No 220, School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067.
authors.forename: Shyamal K
authors.initials: SK
authors.lastname: Goswami
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002148:Calmodulin-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D019143:Evolution, Molecular; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D017434:Protein Structure, Tertiary
chemical_list: D001324:Autoantigens; D002148:Calmodulin-Binding Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C405682:STRN3 protein, human; C485775:Strn3 protein, mouse; C411649:zinedin