title: Polypropylene non-woven meshes with conformal glycosylated layer for lectin affinity adsorption: the effect of side chain length.
abstract: The unique characteristics of polypropylene non-woven meshes (PPNWMs), like random network of overlapped fibers, multiple connected pores and overall high porosity, make them high potentials for use as separation or adsorption media. Meanwhile, carbohydrates can specifically recognize certain lectin through multivalent interactions. Therefore glycosylated PPNWMs, combing the merits of both, can be regarded as superior affinity membranes for lectin adsorption and purification. Here, we describe a versatile strategy for the glycosylation of PPNWMs. Two hydrophilic polymers with different side chain length, poly(2-hydroxyethyl methacrylate) (PHEMA) and poly(oligo(ethylene glycol) methacrylate) (POEGMA), were first conformally tethered on the polypropylene fiber surface by a modified plasma pretreatment and benzophenone (BP) entrapment UV irradiation process. Then glucose ligands were bound through the reaction between the hydroxyl group and acetyl glucose. Chemical changes of the PPNWMs surface were monitored by FT-IR/ATR. SEM pictures show that conformal glucose ligands can be achieved through the modified process. After deprotection, the glycosylated PPNWMs became superhydrophilic and had high specific recognition capability toward Concanavalin A (Con A). Static Con A adsorption experiments were further performed and the results indicate that fast adsorption kinetics and high binding capacity can be accomplished at the same time. We also found that increasing the side chain length of polymer brushes had positive effect on protein binding capacity due to improved chain mobility. Model studies suggest a multilayer adsorption behavior of Con A.
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Xiang-Yu
authors.initials: XY
authors.lastname: Ye
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Huang
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address: xuzk@zju.edu.cn.
authors.forename: Zhi-Kang
authors.initials: ZK
authors.lastname: Xu
mesh_terms: D000327:Adsorption; D001577:Benzophenones / Q000494:pharmacology; D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000737:chemistry*; D005453:Fluorescence; D005947:Glucose / Q000032:analysis; D006031:Glycosylation; D007700:Kinetics; D008024:Ligands; D008689:Methacrylates / Q000737:chemistry; D058626:Plasma Gases / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000737:chemistry; D011109:Polymethacrylic Acids; D011126:Polypropylenes / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D001577:Benzophenones; D008024:Ligands; D008689:Methacrylates; D058626:Plasma Gases; D011109:Polymethacrylic Acids; D011126:Polypropylenes; C528061:poly(poly(ethylene glycol)methacrylate); D003208:Concanavalin A; D011092:Polyethylene Glycols; C005044:hydroxyethyl methacrylate; C047723:benzophenone; D005947:Glucose
keywords: Affinity membrane; Conformal grafting; Lectin adsorption; Polypropylene non-woven mesh; Surface glycosylation