title: The antiproliferative effect of C2-ceramide on lung cancer cells through apoptosis by inhibiting Akt and NFκB.
abstract: The anticancer effects of ceramide have been reported in many types of cancers but less in lung cancer. In this study, we used C2-ceramide to further investigate its possible anticancer effects and mechanisms on non-small cell lung cancer (NSCLC) H1299 cells. The result of cell proliferation in terms of trypan blue assay showed high dose of C2-ceramide inhibited cell survival after 24 h treatment. The flow cytometry-based assays indicated the effect of apoptosis, chromatin condensation, and G1 arrest in terms of Annexin V/propidium iodide (PI), DAPI, and PI stainings, respectively. Moreover, the decreased protein level of p-Akt, p-NFκB, survivin and cyclin A2 were detected by Western blot assay. Taken together, these results indicated the antiproliferative effect of C2-ceramide is majorly responsible for cell apoptosis in lung cancer H1299 cells.
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: I-Ling
authors.initials: IL
authors.lastname: Lin
authors.affiliation: Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.|Department of Biological Sciences, National Sun Yat-Sen University, 70 Lien Hai Road, Kaohsiung 804, Taiwan.
authors.forename: Han-Lin
authors.initials: HL
authors.lastname: Chou
authors.affiliation: Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Jin-Ching
authors.initials: JC
authors.lastname: Lee
authors.affiliation: Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Feng-Wei
authors.initials: FW
authors.lastname: Chen
authors.affiliation: Chest Surgery, Chi-Mei Foundation Medical Center, Yung Kang City, Tainan, 901, Taiwan.
authors.forename: Yao
authors.initials: Y
authors.lastname: Fong
authors.affiliation: Department of Clinical Pharmacy; Master Program for Clinical Pharmacogenomics and Pharmacoproteomics, School of Pharmacy, Taipei Medical University, Taipei, Taiwan.
authors.forename: Wei-Chiao
authors.initials: WC
authors.lastname: Chang
authors.affiliation: Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 804, Taiwan.
authors.forename: Hurng Wern
authors.initials: HW
authors.lastname: Huang
authors.affiliation: Department of Biological Sciences, National Sun Yat-Sen University, 70 Lien Hai Road, Kaohsiung 804, Taiwan.
authors.forename: Chang-Yi
authors.initials: CY
authors.lastname: Wu
authors.affiliation: Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Wen-Tsan
authors.initials: WT
authors.lastname: Chang
authors.affiliation: Department of Fragrance and Cosmetic Science; Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Hui-Min David
authors.initials: HD
authors.lastname: Wang
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.|Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Chien-Chih
authors.initials: CC
authors.lastname: Chiu