title: A live-attenuated and an inactivated chimeric porcine circovirus (PCV)1-2 vaccine are both effective at inducing a humoral immune response and reducing PCV2 viremia and intrauterine infection in female swine of breeding age.
abstract: The objective of this pilot study was to determine the efficacy of inactivated (1 or 2 dose) and live-attenuated chimeric porcine circovirus (PCV)1-2 vaccines in sows using the PCV2-spiked semen model. Thirty-five sows were randomly divided into 6 groups: negative and positive controls, 1 dose inactivated PCV1-2 vaccine challenged (1-VAC-PCV2), 2 dose inactivated PCV1-2 vaccine challenged (2-VAC-PCV2), 1 dose live-attenuated PCV1-2 vaccine unchallenged (1-LIVE-VAC), and 1 dose live-attenuated PCV1-2 vaccine challenged (1-LIVE-VAC-PCV2). The inactivated PCV1-2 vaccine induced higher levels of PCV2-specific antibodies in dams. All vaccination strategies provided good protection against PCV2 viremia in dams, whereas the majority of the unvaccinated sows were viremic. Four of the 35 dams became pregnant: a negative control, a positive control, a 2-VAC-PCV2 sow, and a 1-LIVE-VAC-PCV2 sow. The PCV2 DNA was detected in 100%, 67%, and 29% of the fetuses obtained from the positive control, inactivated vaccinated, or live-attenuated vaccinated dams, respectively. The PCV2 antigen in hearts was only detectable in the positive control litter (23% of the fetuses). The PCV1-2 DNA was detected in 29% of the fetuses in the litter from the 1-LIVE-VAC-PCV2 dam. Under the conditions of this pilot study, both vaccines protected against PCV2 viremia in breeding age animals; however, vertical transmission was not prevented.
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine (Hemann, Wang, Halbur, Opriessnig) and Department of Statistics, College of Liberal Arts & Sciences, Iowa State University, Ames, Iowa, USA (Wang); Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA (Beach, Meng); The Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, United Kingdom (Opriessnig).
authors.forename: Michelle
authors.initials: M
authors.lastname: Hemann
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine (Hemann, Wang, Halbur, Opriessnig) and Department of Statistics, College of Liberal Arts & Sciences, Iowa State University, Ames, Iowa, USA (Wang); Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA (Beach, Meng); The Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, United Kingdom (Opriessnig).
authors.forename: Nathan M
authors.initials: NM
authors.lastname: Beach
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine (Hemann, Wang, Halbur, Opriessnig) and Department of Statistics, College of Liberal Arts & Sciences, Iowa State University, Ames, Iowa, USA (Wang); Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA (Beach, Meng); The Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, United Kingdom (Opriessnig).
authors.forename: Xiang-Jin
authors.initials: XJ
authors.lastname: Meng
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine (Hemann, Wang, Halbur, Opriessnig) and Department of Statistics, College of Liberal Arts & Sciences, Iowa State University, Ames, Iowa, USA (Wang); Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA (Beach, Meng); The Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, United Kingdom (Opriessnig).
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine (Hemann, Wang, Halbur, Opriessnig) and Department of Statistics, College of Liberal Arts & Sciences, Iowa State University, Ames, Iowa, USA (Wang); Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA (Beach, Meng); The Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, United Kingdom (Opriessnig).
authors.forename: Patrick G
authors.initials: PG
authors.lastname: Halbur
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine (Hemann, Wang, Halbur, Opriessnig) and Department of Statistics, College of Liberal Arts & Sciences, Iowa State University, Ames, Iowa, USA (Wang); Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA (Beach, Meng); The Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, United Kingdom (Opriessnig).
authors.forename: Tanja
authors.initials: T
authors.lastname: Opriessnig
mesh_terms: D000375:Aging; D000818:Animals; D018173:Circoviridae Infections / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000276:immunology*; D005260:Female; D056724:Immunity, Humoral / Q000276:immunology*; D011247:Pregnancy; D013552:Swine; D014591:Uterine Diseases / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D014613:Vaccines, Attenuated; D015164:Vaccines, Inactivated; D014765:Viral Vaccines / Q000276:immunology*; D014766:Viremia / Q000662:veterinary*
chemical_list: D014613:Vaccines, Attenuated; D015164:Vaccines, Inactivated; D014765:Viral Vaccines