title: Screening of osteoanagenesis-active compounds from Scutellaria baicalensis Georgi by hPDLC/CMC-online-HPLC/MS.
abstract: In present study, an online analytical method using human periodontal ligament cell/cell membrane chromatography (hPDLC/CMC) combined with high-performance liquid chromatography/mass spectrometry (HPLC/MS) was used for direct recognition, separation, and identification of compounds for the first time from Scutellaria baicalensis Georgi (SBG) that are active on hPDLCs. Baicalein (BAI) and wogonin (WOG), which were identified as the active compounds of the ethyl ether extract of S. baicalensis Georgi (SBGEE), could bind to the same membrane receptor of hPDLC for simvastatin (SIM). Moreover, BAI (0.15-0.6 mg/L) and WOG (0.015-0.6 mg/L) had the capability to enhance cell proliferation, matrix calcification, and formation of calcified nodules, which are comparable to the activities of SIM (0.1 mg/L) in vitro. These observations are consistent with the K(A) of the various drugs. It is very important for the development of SBG used to treat periodontitis.
authors.affiliation: Department of Periodontology, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710 004, China; School of Medicine, Xi'an Jiaotong University, Xi'an 710 061, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.affiliation: School of Medicine, Xi'an Jiaotong University, Xi'an 710 061, China.
authors.forename: Sicen
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Periodontology, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710 004, China.
authors.forename: Junyi
authors.initials: J
authors.lastname: Sun
authors.affiliation: Research Center for Stomatology, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710 004, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Periodontology, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710 004, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Periodontology, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710 004, China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Gou
authors.affiliation: Department of Periodontology, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710 004, China; Research Center for Stomatology, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710 004, China. Electronic address: drliangyj@gmail.com.
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.affiliation: School of Medicine, Xi'an Jiaotong University, Xi'an 710 061, China. Electronic address: helc@mail.xjtu.edu.cn.
authors.forename: Langchong
authors.initials: L
authors.lastname: He
mesh_terms: D000293:Adolescent; D000328:Adult; D001681:Biological Assay; D002113:Calcification, Physiologic / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D044950:Flavanones / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D013058:Mass Spectrometry; D010012:Osteogenesis / Q000187:drug effects*; D010513:Periodontal Ligament / Q000187:drug effects; D010518:Periodontitis / Q000188:drug therapy*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D031364:Scutellaria baicalensis / Q000737:chemistry*; D055815:Young Adult
chemical_list: D044950:Flavanones; D010936:Plant Extracts; C499814:Scutellaria baicalensis extract; C006680:baicalein; C085514:wogonin
keywords: Baicalein; High-performance liquid chromatography/mass spectrometry; Human periodontal ligament cell membrane chromatography; Scutellaria baicalensis Georgi; Wogonin