title: Human bocavirus isolated from children with acute respiratory tract infections in Korea, 2010-2011.
abstract: Human bocavirus (HBoV) was first recognized in respiratory samples in 2005. The clinical importance of HBoV infection remains unclear. This report describes the clinical features and molecular phylogeny of HBoV isolates in children with acute respiratory infections. Nasopharyngeal aspirates were obtained from 1,528 children with acute respiratory infections between 2010 and 2011. Respiratory samples were screened for HBoV by multiplex PCR. A phylogenetic analysis of the HBoV VP1/VP2 gene was also undertaken. HBoV was detected in 187 (12.2%) of the 1,528 patients with a peak incidence of infection observed in patients aged 12-24 months. Coinfection with other respiratory viruses was observed in 107 (57.2%) of the HBoV-positive children. The peak of HBoV activity occurred during the month of June in both 2010 and 2011. A higher previous history of wheezing (P = 0.016), a higher frequency of chest retraction (P < 0.001) and wheezing (P = 0.022), a higher respiratory symptom score (P = 0.002), and a longer duration of hospital stay (P = 0.021) were observed in HBoV-positive children compared with the HBoV-negative group. Phylogenetic analysis showed all 187 HBoV-positive isolates were identified as HBoV 1, indicating minimal sequence variations among the isolates. A single lineage of HBoV 1 was found to have circulated in children with acute respiratory infections between 2010 and 2011 and was associated with several clinical characteristics including age, seasonality, and clinical severity with retraction, wheezing, and longer hospitalization. The clinical relevance of the minimal sequence variations of HBoV remains to be determined.
authors.affiliation: Department of Pediatrics, School of Medicine, Ewha Womans University, Seoul, Korea; Department of Medicine, The Graduate School of Yonsei University, Seoul, Korea.
authors.forename: Jong Gyun
authors.initials: JG
authors.lastname: Ahn
authors.forename: Seong Yeol
authors.initials: SY
authors.lastname: Choi
authors.forename: Dong Soo
authors.initials: DS
authors.lastname: Kim
authors.forename: Ki Hwan
authors.initials: KH
authors.lastname: Kim
mesh_terms: D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D060085:Coinfection / Q000453:epidemiology / Q000821:virology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D056661:Human bocavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D060885:Multiplex Polymerase Chain Reaction; D009305:Nasopharynx / Q000821:virology; D010322:Parvoviridae Infections / Q000453:epidemiology* / Q000473:pathology / Q000821:virology*; D010802:Phylogeny; D015995:Prevalence; D056910:Republic of Korea / Q000453:epidemiology; D012141:Respiratory Tract Infections / Q000453:epidemiology* / Q000473:pathology / Q000821:virology*; D012621:Seasons; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D015678:Viral Structural Proteins
keywords: acute respiratory infections; human bocavirus; phylogenetic analysis