title: The use of oligonucleotide recombination to generate isogenic mutants of clinical isolates of Pseudomonas aeruginosa.
abstract: Oligonucleotide recombination allows the introduction of specific point mutations into the bacterial genome. Here we report the successful application of this technique to clinical isolates of Pseudomonas aeruginosa to allow subsequent investigations of the biological impact associated with resistance-conferring mutations.
authors.affiliation: University of Southern California, School of Pharmacy, 1985 Zonal Avenue, Los Angeles, CA 90033.
authors.forename: Melissa
authors.initials: M
authors.lastname: Agnello
authors.affiliation: University of Southern California, School of Pharmacy, 1985 Zonal Avenue, Los Angeles, CA 90033. Electronic address: anniew@usc.edu.
authors.forename: Annie
authors.initials: A
authors.lastname: Wong-Beringer
mesh_terms: D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D009841:Oligonucleotides / Q000235:genetics*; D017354:Point Mutation / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics*
chemical_list: D009841:Oligonucleotides
keywords: Fluoroquinolones; Pseudomonas aeruginosa; Recombination; Resistance mutations