title: Pigs fed camelina meal increase hepatic gene expression of cytochrome 8b1, aldehyde dehydrogenase, and thiosulfate transferase.
abstract: Camelina sativa is an oil seed crop which can be grown on marginal lands. Camelina seed oil is rich in omega-3 fatty acids (>35%) and γ-tocopherol but is also high in erucic acid and glucosinolates. Camelina meal, is the by-product after the oil has been extracted. Camelina meal was fed to 28 d old weaned pigs at 3.7% and 7.4% until age 56 d. The camelina meal supplements in the soy based diets, improved feed efficiency but also significantly increased the liver weights. Gene expression analyses of the livers, using intra-species microarrays, identified increased expression of phase 1 and phase 2 drug metabolism enzymes. The porcine versions of the enzymes were confirmed by real time PCR. Cytochrome 8b1 (CYP8B1), aldehyde dehydrogenase 2 (Aldh2), and thiosulfate transferase (TST) were all significantly stimulated. Collectively, these genes implicate the camelina glucosinolate metabolite, methyl-sulfinyldecyl isothiocyanate, as the main xeniobiotic, causing increased hepatic metabolism and increased liver weight.
authors.affiliation: AAFC-Lacombe, 6000 C&E Trail, Lacombe, AB, Canada T4L 1 W1. jon.meadus@agr.gc.ca.
authors.forename: William Jon
authors.initials: WJ
authors.lastname: Meadus
authors.forename: Pascale
authors.initials: P
authors.lastname: Duff
authors.forename: Tanya
authors.initials: T
authors.lastname: McDonald
authors.forename: William R
authors.initials: WR
authors.lastname: Caine