title: Identification of Tmem10 as a novel late-stage oligodendrocytes marker for detecting hypomyelination.
abstract: Oligodendrocytes ensheath axons to form compact insulating multilamellar structures known as myelin. Tmem10 is a novel type I transmembrane glycoprotein that is highly expressed in oligodendrocytes and whose biological function remains largely unknown. Furthermore, the expression pattern of Tmem10 remains a matter of some controversy. Given the inconsistency of its expression pattern and the lack of validated specific antibodies, Tmem10 is not widely accepted as a marker for mature oligodendrocytes. As a means to solve these problems and to aid future studies of oligodendrocyte-associated diseases, we have generated a highly specific Tmem10 antibody. Using this Tmem10 antibody, we clarify that Tmem10 protein is firstly expressed at 2 weeks in the postnatal mouse brain with age-related increase, only in the central nervous system (CNS). We also reveal that Tmem10 is expressed specifically in late stage oligodendrocytes and later than MAG, a late-stage myelin marker. Finally, we show that Tmem10 co-expresses with MOG- and MBP-positive myelin fibers and is dramatically reduced in a hypomyelination mouse model. In conclusion, our study demonstrates that Tmem10 can be used as a specific marker for myelinating oligodendrocytes and perhaps for the evaluation of myelination diseases, such as multiple sclerosis.
authors.affiliation: 1. State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Wanxiang
authors.initials: W
authors.lastname: Jiang
authors.affiliation: 1. State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Wanchun
authors.initials: W
authors.lastname: Yang
authors.affiliation: 1. State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Yang
authors.affiliation: 1. State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Junyan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: 1. State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Dejiang
authors.initials: D
authors.lastname: Pang
authors.affiliation: 1. State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Lingxue
authors.initials: L
authors.lastname: Gan
authors.affiliation: 1. State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Luo
authors.affiliation: 2. Department of neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Fan
authors.affiliation: 2. Department of neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Liu
authors.affiliation: 1. State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Mina
authors.initials: M
authors.lastname: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence; D015415:Biomarkers / Q000378:metabolism*; D002490:Central Nervous System / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers; D051379:Mice; D008810:Mice, Inbred C57BL; D009185:Myelin Proteins / Q000235:genetics / Q000378:metabolism*; D009186:Myelin Sheath / Q000378:metabolism*; D009836:Oligodendroglia / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D017931:DNA Primers; D009185:Myelin Proteins; C524019:Opalin protein, mouse
keywords: Myelination; Tmem10; late stage oligodendrocytes maker; polyclonal antibody