title: Four conventional soybean [Glycine max (L.) Merrill] seeds exhibit different protein profiles as revealed by proteomic analysis.
abstract: Soybeans have several functional properties due to their composition and may exert beneficial health effects that are attributed to proteins and their derivative peptides. The present study aimed to analyze the protein profiles of four new conventional soybean seeds (BRS 257, BRS 258, BRS 267, and Embrapa 48) with the use of proteomic tools. Two-dimensional (2D) and one-dimensional (1D) gel electrophoreses were performed, followed by MALDI-TOF/TOF and ESI-Q-TOF mass spectrometry analyses, respectively. These two different experimental approaches allowed the identification of 117 proteins from 1D gels and 46 differentially expressed protein spots in 2D gels. BRS 267 showed the greatest diversity of identified spots in the 2D gel analyses. In the 1D gels, the major groups were storage (25-40%) and lipid metabolism (11-25%) proteins. The differences in protein composition between cultivars could indicate functional and nutritional differences and could direct the development of new cultivars.
authors.affiliation: Departamento de Nutrição Básica e Experimental, Instituto de Nutrição Josué de Castro, Universidade Federal do Rio de Janeiro , Av. Carlos Chagas Filho, Prédio do CCS, Bloco J-2, Laboratório 13, 393 Rio de Janeiro 21941-590, Brazil.
authors.forename: Luciana S
authors.initials: LS
authors.lastname: Gomes
authors.forename: Raquel
authors.initials: R
authors.lastname: Senna
authors.forename: Vanessa
authors.initials: V
authors.lastname: Sandim
authors.forename: Mário A C
authors.initials: MA
authors.lastname: Silva-Neto
authors.forename: Jonas E A
authors.initials: JE
authors.lastname: Perales
authors.forename: Russolina B
authors.initials: RB
authors.lastname: Zingali
authors.forename: Márcia R
authors.initials: MR
authors.lastname: Soares
authors.forename: Eliane
authors.initials: E
authors.lastname: Fialho
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D040901:Proteomics; D012639:Seeds / Q000737:chemistry*; D030262:Soybean Proteins / Q000032:analysis*; D013025:Glycine max / Q000737:chemistry*; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D030262:Soybean Proteins