title: Gene expression of IGF1, IGF1R, and IGFBP3 in epiretinal membranes of patients with proliferative diabetic retinopathy: preliminary study.
abstract: The molecular mechanism formation of secondary epiretinal membranes (ERMs) after proliferative diabetic retinopathy (PDR) or primary idiopathic ERMs is still poorly understood. Therefore, the present study focused on the assessment of IGF1, IGF1R, and IGFBP3 mRNA levels in ERMs and PBMCs from patients with PDR. The examined group comprised 6 patients with secondary ERMs after PDR and the control group consisted of 11 patients with idiopathic ERMs. Quantification of IGF1, IGF1R, and IGFBP3 mRNAs was performed by real-time QRT-PCR technique. In ERMs, IGF1 and IGF1R mRNA levels were significantly higher in patients with diabetes compared to control subjects. In PBMCs, there were no statistically significant differences of IGF1, IGF1R, and IGFBP3 expression between diabetic and nondiabetic patients. In conclusion, our study indicated IGF1 and IGF1R differential expression in ERMs, but not in PBMCs, of diabetic and nondiabetic patients, suggesting that these factors can be involved in the pathogenesis or progression of proliferative vitreoretinal disorders. This trial is registered with NCT00841334.
authors.affiliation: Clinical Department of Ophthalmology, Medical University of Silesia, Ceglana Street 35, 40-952 Katowice, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Romaniuk
authors.affiliation: Department of Molecular Biology, Medical University of Silesia, Narcyzów Street 1, 41-200 Sosnowiec, Poland.
authors.forename: Malgorzata W
authors.initials: MW
authors.lastname: Kimsa
authors.affiliation: Department of Molecular Biology, Medical University of Silesia, Narcyzów Street 1, 41-200 Sosnowiec, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Strzalka-Mrozik
authors.affiliation: Department of Molecular Biology, Medical University of Silesia, Narcyzów Street 1, 41-200 Sosnowiec, Poland.
authors.forename: Magdalena C
authors.initials: MC
authors.lastname: Kimsa
authors.affiliation: Clinical Department of Ophthalmology, Medical University of Silesia, Ceglana Street 35, 40-952 Katowice, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Kabiesz
authors.affiliation: Clinical Department of Ophthalmology, Medical University of Silesia, Ceglana Street 35, 40-952 Katowice, Poland.
authors.forename: Wanda
authors.initials: W
authors.lastname: Romaniuk
authors.affiliation: Department of Molecular Biology, Medical University of Silesia, Narcyzów Street 1, 41-200 Sosnowiec, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Mazurek
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D003930:Diabetic Retinopathy / Q000209:etiology* / Q000378:metabolism / Q000601:surgery; D019773:Epiretinal Membrane / Q000378:metabolism*; D005260:Female; D006801:Humans; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000235:genetics / Q000502:physiology*; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D060888:Real-Time Polymerase Chain Reaction; D017526:Receptor, IGF Type 1 / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018630:Vitreoretinopathy, Proliferative / Q000209:etiology
chemical_list: C583557:IGF1 protein, human; C515497:IGFBP3 protein, human; D018972:Insulin-Like Growth Factor Binding Protein 3; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1