title: Determination of nifedipine in dog plasma by high-performance liquid chromatography with tandem mass spectrometric detection.
abstract: Nifedipine is a dihydropyridine calcium channel blocker used widely in the management of hypertension and other cardiovascular disorders. In this work, a simple, rapid and sensitive liquid chromatography/tandem mass spectrometry method was developed and validated to determine nifedipine in dog plasma using nimodipine as the internal standard. Chromatographic separation was carried out on a C₈ column. The mobile phase consisted of a mixture of acetonitrile, water and formic acid (60:40:0.2, v/v/v) at a flow rate of 0.5 mL/min. Detection was performed on a triple quadrupole tandem mass spectrometer in selected reaction monitoring mode via an atmospheric pressure chemical ionization source. The method has a lower limit of quantification of 0.20 ng/mL with consumption of plasma as low as 0.05 mL. The linear calibration curves were obtained in the concentration range of 0.20-50.0 ng/mL (r = 0.9948). The recoveries of the liquid extraction method were 74.5-84.1%. Intra-day and inter-day precisions were 4.1-8.8 and 6.7-7.4%, respectively. The quantification was not interfered with by other plasma components and the method was applied to determine nifedipine in plasma after a single oral administration of two controlled-release nifedipine tablets to beagle dogs.
authors.affiliation: School of Pharmaceutical Science, Wuhan University, 185 Donghu Road, Wuhan, 430071, People's Republic of China; Department of Pharmacy, Remin Hospital of Wuhan University, 238 Jiefang Road, Wuhan, 430060, People's Republic of China.
authors.forename: Xigui
authors.initials: X
authors.lastname: Pan
authors.forename: Shunchang
authors.initials: S
authors.lastname: Zhou
authors.forename: Qinqin
authors.initials: Q
authors.lastname: Fu
authors.forename: Xianming
authors.initials: X
authors.lastname: Hu
authors.forename: Jianhong
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D016014:Linear Models; D008297:Male; D009543:Nifedipine / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009543:Nifedipine
keywords: LC-MS/MS; beagle dog; nifedipine; nimodipine; pharmacokinetics