title: Analysis of biological networks and biological pathways associated with residual feed intake in beef cattle.
abstract: In this study, biological networks were reconstructed from genes and metabolites significantly associated with residual feed intake (RFI) in beef cattle. The networks were then used to identify biological pathways associated with RFI. RFI is a measure of feed efficiency, which is independent of body size and growth; therefore selection for RFI is expected to result in cattle that consume less feed without adverse effects on growth rate and mature size. Although several studies have identified genes associated with RFI, the mechanisms of the biological processes are not well understood. In this study, we utilised the results obtained from two association studies, one using 24 genes and one using plasma metabolites to reconstruct biological networks associated with RFI using IPA software (Igenuity Systems). The results pointed to biological processes such as lipid and steroid biosynthesis, protein and carbohydrate metabolism and regulation of gene expression through DNA transcription, protein stability and degradation. The major canonical pathways included signaling of growth hormone, Oncostatin M, insulin-like growth factor and AMP activated protein kinase, and cholesterol biosynthesis. This study provides information on potential biological mechanisms, and genes and metabolites involved in feed efficiency in beef cattle.
authors.affiliation: Livestock Gentec, Department of Agriculture, Food and Nutritional Science, 4.10 Agriculture Forestry Center, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Brian
authors.initials: B
authors.lastname: Karisa
authors.forename: Stephen
authors.initials: S
authors.lastname: Moore
authors.forename: Graham
authors.initials: G
authors.lastname: Plastow
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena / Q000235:genetics* / Q000502:physiology*; D000818:Animals; D050260:Carbohydrate Metabolism / Q000235:genetics; D002417:Cattle / Q000378:metabolism* / Q000502:physiology*; D002784:Cholesterol / Q000096:biosynthesis; D004435:Eating / Q000235:genetics*; D005786:Gene Expression Regulation; D013006:Growth Hormone / Q000378:metabolism; D050356:Lipid Metabolism / Q000235:genetics; D053683:Oncostatin M / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D013002:Somatomedins / Q000378:metabolism
chemical_list: D011506:Proteins; D013002:Somatomedins; D053683:Oncostatin M; D013006:Growth Hormone; D002784:Cholesterol; D055372:AMP-Activated Protein Kinases
keywords: beef cattle; biological networks; genes; metabolites; residual feed intake