title: Evaluation of vancomycin-based synergistic system with amino acid ester chiral ionic liquids as additives for enantioseparation of non-steroidal anti-inflammatory drugs by capillary electrophoresis.
abstract: Recently, chiral ionic liquids (ILs) have drawn increasing attention in chiral separation field; however, few papers reported the application of chiral ILs for chiral separation by capillary electrophoresis (CE), and among the papers, chiral ILs were mainly applied as additives to β-cyclodextrin derivatives systems to establish synergistic systems. The synergistic system based on antibiotics with chiral ILs as additives has never been reported before. In this paper, two chiral ionic liquids (ILs) based on amino acid ester, L-alanine and L-valine tert butyl ester bis (trifluoromethane) sulfonamide, were first applied to evaluate the synergistic effect with antibiotic selector for CE chiral separation. The vancomycin-based synergistic system with chiral ILs as additives was successfully established and investigated for the enantioseparation of naproxen, carprofen, ibuprofen, ketoprofen and pranoprofen. Compared to vancomycin-alone cases, significant improvement of separation for all the analytes was observed. Several parameters, such as type and proportion of organic modifier, composition and pH of buffer, concentration of chiral ILs and vancomycin were systematically investigated, and then evaluated by means of Statistical Product and Service Solutions (SPSS) to research the influences on the synergistic effect. Finally, the established method was successfully applied to test the chiral impurity of naproxen sample.
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Jinjing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, PR China; Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing 210009, PR China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China. Electronic address: du_yingxiang@126.com.
authors.forename: Yingxiang
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Physical Chemistry, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Lei
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification*; D002021:Buffers; D004357:Drug Synergism; D019075:Electrophoresis, Capillary / Q000379:methods*; D004952:Esters; D006863:Hydrogen-Ion Concentration; D052578:Ionic Liquids / Q000737:chemistry*; D057230:Limit of Detection; D013237:Stereoisomerism; D014640:Vancomycin / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D000894:Anti-Inflammatory Agents, Non-Steroidal; D002021:Buffers; D004952:Esters; D052578:Ionic Liquids; D014640:Vancomycin
keywords: Amino acid ester derived chiral ionic liquids; Capillary electrophoresis; Chiral ILs; Chiral separations; NSAIDs; Non-steroidal anti-inflammatory drugs; chiral ionic liquids; l-AlaC(4)NTf(2); l-ValC(4)NTf(2); l-alanine tert butyl ester bis (trifluoromethane) sulfonamide; l-valine tert butyl ester bis (trifluoromethane) sulfonamide; non-steroidal anti-inflammatory drugs