title: Novel and rapid enumeration method of peripheral blood stem cells using automated hematology analyzer.
abstract: BACKGROUND
  The number of infused CD34(+) cells is crucial to the success of peripheral blood stem cell transplantation (PBSCT). Here, we present, for the first time, a new method of enumerating hematopoietic progenitor cells (HPCs) for PBSCT.
  
  
  METHODS
  This novel method is based on hemolysis and chemical staining, followed by flow cytometry-based optical detection, conducted using an automated hematology analyzer (XN series, Sysmex). CD34(+) cells and HPCs were compared in 76 granulocyte colony-stimulating factor (G-CSF)-mobilized blood or apheresis samples taken from healthy donors (n = 18) or patients undergoing autologous PBSCT (n = 6).
  
  
  RESULTS
  There was a strong correlation between the numbers of HPCs and CD34(+) cells (R(2)  = 0.958). The expected total number of HPCs in the final products, which was estimated from HPCs in pre-apheresis PB or mid-apheresis products, also correlated well with the total number of CD34(+) cells in the final products. The change in HPCs in PB closely resembled that of CD34(+) cells during mobilization. Experiments using immunomagnetic beads suggested that the majority of CD34(+) cells existed in HPCs, and vice versa.
  
  
  CONCLUSIONS
  Hematopoietic progenitor cells may serve as surrogates for CD34(+) cells in PBSCT. However, further investigations are required to verify this.
authors.affiliation: Department of Blood Transfusion and Cellular Therapy, National Cancer Center Hospital, Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Tanosaki
authors.forename: T
authors.initials: T
authors.lastname: Kumazawa
authors.forename: A
authors.initials: A
authors.lastname: Yoshida
authors.forename: S
authors.initials: S
authors.lastname: Oguni
authors.forename: A
authors.initials: A
authors.lastname: Nakano
authors.forename: S
authors.initials: S
authors.lastname: Yamagata
authors.forename: N
authors.initials: N
authors.lastname: Takahashi
authors.forename: S
authors.initials: S
authors.lastname: Kurosawa
authors.forename: S W
authors.initials: SW
authors.lastname: Kim
authors.forename: T
authors.initials: T
authors.lastname: Yamashita
authors.forename: S
authors.initials: S
authors.lastname: Mori
authors.forename: Y
authors.initials: Y
authors.lastname: Heike
authors.forename: T
authors.initials: T
authors.lastname: Fukuda
authors.forename: Y
authors.initials: Y
authors.lastname: Hamaguchi
authors.forename: H
authors.initials: H
authors.lastname: Tsuda
mesh_terms: D018952:Antigens, CD34 / Q000097:blood; D057205:Automation, Laboratory; D015415:Biomarkers / Q000097:blood; D001772:Blood Cell Count / Q000295:instrumentation / Q000379:methods*; D001773:Blood Cells / Q000166:cytology*; D016022:Case-Control Studies; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D019337:Hematologic Neoplasms / Q000473:pathology / Q000628:therapy*; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006461:Hemolysis; D006801:Humans; D036102:Peripheral Blood Stem Cell Transplantation; D014182:Transplantation, Autologous
chemical_list: D018952:Antigens, CD34; D015415:Biomarkers; D016179:Granulocyte Colony-Stimulating Factor
keywords: HPC; PBSCT; automated hematology analyzer; hematopoietic stem cell transplantation