title: Decacationic [70]Fullerene Approach for Efficient Photokilling of Infectious Bacteria and Cancer Cells.
abstract: Photodynamic inactivation of pathogenic bacteria and cancer cells by novel water-soluble decacationic fullerene monoadducts, C60[>M(C3N6+C3)2] and C70[>M(C3N6+C3)2], were investigated. In the presence of a high number of electron-donating iodide anions as parts of quaternary ammonium salts in the arm region, we found that C70[>M(C3N6+C3)2] produced more highly reactive HO•radical than C60[>M(C3N6+C3)2], in addition to singlet oxygen (1O2). This finding offers an explanation of the preferential killing of Gram-positive and Gram-negative bacteria by C60[>M(C3N6+C3)2] and C70[>M(C3N6+C3)2], respectively. The hypothesis is that1O2can diffuse more easily into porous cell walls of Gram-positive bacteria to reach sensitive sites, while the less permeable Gram-negative bacterial cell wall needs the more reactive HO•to cause real damage.
authors.affiliation: Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA 02114, USA ; Department of Dermatology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: L
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Chemistry, University of Massachusetts, Lowell, MA 01854, USA.
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.affiliation: Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA 02114, USA.
authors.forename: S K
authors.initials: SK
authors.lastname: Sharma
authors.affiliation: Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA 02114, USA ; Department of Dermatology, Harvard Medical School, Boston, MA 02115, USA ; School of Dentistry, University of Sao Paulo, 05508-000, Brazil ; CAPES Foundation, Ministry of Education of Brazil, Brasília, DF 70040-020, Brazil.
authors.forename: F F
authors.initials: FF
authors.lastname: Sperandio
authors.affiliation: Department of Chemistry, University of Massachusetts, Lowell, MA 01854, USA.
authors.forename: S
authors.initials: S
authors.lastname: Maragani
authors.affiliation: Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA 02114, USA.
authors.forename: S
authors.initials: S
authors.lastname: Nayka
authors.affiliation: Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA 02114, USA ; Department of Chemistry, Harvard University, Cambridge, MA 02138, USA.
authors.forename: J
authors.initials: J
authors.lastname: Chang
authors.affiliation: Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA 02114, USA ; Department of Dermatology, Harvard Medical School, Boston, MA 02115, USA ; Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA 02139, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Hamblin
authors.affiliation: Department of Chemistry, University of Massachusetts, Lowell, MA 01854, USA ; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON M5S 1A8, Canada.
authors.forename: L Y
authors.initials: LY
authors.lastname: Chiang