title: Recombinant porcine surfactant protein D inhibits influenza A virus replication ex vivo.
abstract: Influenza is a major burden to public health. Due to high mutation rates and selection pressure, mutant viruses emerge which are resistant to currently used antiviral drugs. Therefore, there is a need for the development of novel classes of antiviral drugs that suffer less from the emergence of resistant viruses. Antiviral drugs based on collectin-like surfactant protein D (SP-D) may fulfil these requirements. Especially porcine SP-D displays strong antiviral activity to influenza A viruses. In the present study the antiviral activity of recombinant porcine SP-D was investigated in ex vivo cultures of respiratory tract tissue infected with human influenza A virus of the H3N2 subtype. Porcine SP-D has antiviral activity in these test systems. It is suggested that porcine SP-D may be used as a venue to develop a novel class of antiviral drugs.
authors.affiliation: Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands.
authors.forename: Marine L B
authors.initials: ML
authors.lastname: Hillaire
authors.affiliation: Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
authors.forename: Martin
authors.initials: M
authors.lastname: van Eijk
authors.affiliation: Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands.
authors.forename: Stella E
authors.initials: SE
authors.lastname: Vogelzang-van Trierum
authors.affiliation: Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands.
authors.forename: Ron A M
authors.initials: RA
authors.lastname: Fouchier
authors.affiliation: Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands; Viroclinics Biosciences BV, Rotterdam, The Netherlands.
authors.forename: Albert D M E
authors.initials: AD
authors.lastname: Osterhaus
authors.affiliation: Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
authors.forename: Henk P
authors.initials: HP
authors.lastname: Haagsman
authors.affiliation: Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands; Viroclinics Biosciences BV, Rotterdam, The Netherlands. Electronic address: g.rimmelzwaan@erasmusmc.nl.
authors.forename: Guus F
authors.initials: GF
authors.lastname: Rimmelzwaan
mesh_terms: D000818:Animals; D009980:Influenza A virus / Q000187:drug effects* / Q000502:physiology*; D008168:Lung / Q000473:pathology / Q000821:virology; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000821:virology; D037663:Pulmonary Surfactant-Associated Protein D / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology*; D013552:Swine; D014132:Trachea / Q000473:pathology / Q000821:virology; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D037663:Pulmonary Surfactant-Associated Protein D; D011994:Recombinant Proteins
keywords: Antiviral activity; Influenza A virus; Porcine surfactant protein D