title: The roles of acid-sensing ion channel 1a and ovarian cancer G protein-coupled receptor 1 on passive Mg2+ transport across intestinal epithelium-like Caco-2 monolayers.
abstract: Intestinal passive Mg(2+) absorption, which is vital for normal Mg(2+) homeostasis, has been shown to be regulated by luminal proton. We aimed to study the regulatory role of intestinal acid sensors in paracellular passive Mg(2+) transport. Omeprazole enhanced the expressions of acid-sensing ion channel 1a (ASIC1a), ovarian cancer G protein-coupled receptor 1 (OGR1), and transient receptor potential vanilloid 4 in Caco-2 cells. It also inhibited passive Mg(2+) transport across Caco-2 monolayers. The expression and activation of OGR1 resulted in the stimulation of passive Mg(2+) transport via phospholipase C- and protein kinase C-dependent pathways. ASIC1a activation, on the other hand, enhanced apical HCO3 (-) secretion that led, at least in part, by a Ca(2+)-dependent pathway to an inhibition of paracellular Mg(2+) absorption. Our results provided supporting evidence for the roles of OGR1 and ASIC1a in the regulation of intestinal passive Mg(2+) absorption.
authors.affiliation: Division of Physiology, Department of Biomedical Sciences, Faculty of Allied Health Sciences, Burapha University, 169 Long-Hard Bangsaen Rd., Saensook, Muang, Chonburi, 20131, Thailand, narongritt@buu.ac.th.
authors.forename: Narongrit
authors.initials: N
authors.lastname: Thongon
authors.forename: Pattamaporn
authors.initials: P
authors.lastname: Ketkeaw
authors.forename: Chanin
authors.initials: C
authors.lastname: Nuekchob
mesh_terms: D062565:Acid Sensing Ion Channels / Q000378:metabolism*; D001639:Bicarbonates / Q000378:metabolism; D018938:Caco-2 Cells; D002118:Calcium / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D017136:Ion Transport; D008274:Magnesium / Q000378:metabolism*; D009853:Omeprazole / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D013997:Time Factors; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: C568266:ASIC1 protein, human; D062565:Acid Sensing Ion Channels; D001639:Bicarbonates; C486281:GPR68 protein, human; D043562:Receptors, G-Protein-Coupled; D011493:Protein Kinase C; D010738:Type C Phospholipases; D008274:Magnesium; D009853:Omeprazole; D002118:Calcium