title: miRNAs expression profile in bast of ramie elongation phase and cell wall thickening and end wall dissolving phase.
abstract: MicroRNAs (miRNAs) are 20-24 nucleotide long non-coding RNAs that play critical regulatory roles during plant development, organ morphogenesis, and cell fate determination and differentiation. In this study, miRNA microarray chips were used to explore the expression profile of ramie miRNAs between the bast of fiber elongation phase and those of cell wall thickening and end wall dissolving phase. There are 150 and 148 credible miRNAs in the bast of fiber elongation phase and cell wall thickening and end wall dissolving phase, respectively. These miRNAs distributed in 27 species and mainly concentrated in nine species. Analysis showed that 51 miRNAs were differentially expressed: 27 up-regulated (miR166, miR172, miR396, miR482, miR894 and miR2911 families) and 24 down-regulated (miR156, miR159, miR164, miR319 and miR1450 families) in the bast of fiber elongation phase compared with the bast of cell wall thickening and end wall dissolving phase. To further confirm our results, we examined the expression of three miRNAs (zma-miR172b*, pvu-miR482 and vvi-172a) by quantitative real-time reverse transcriptase-PCR. Our results will provide a molecular basis for future research miRNA function on ramie genetics and breeding.
authors.affiliation: Department of Bio-engineering, Faulty of Environmental Engineering, Engineering Research Center for Cleaner Production of Textile Dyeing and Printing, Ministry of Education, Wuhan Textile University, Wuhan, 430073, China, junwang2100@hotmail.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Jing-Shu
authors.initials: JS
authors.lastname: Huang
authors.forename: Xin-Yan
authors.initials: XY
authors.lastname: Hao
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Feng
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Cai
authors.forename: Li-Qin
authors.initials: LQ
authors.lastname: Sun
mesh_terms: D032402:Boehmeria / Q000235:genetics* / Q000254:growth & development; D002473:Cell Wall / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D018749:RNA, Plant / Q000096:biosynthesis / Q000235:genetics*; D013312:Stress, Physiological; D059467:Transcriptome
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant