title: The critical threshold of Lawsonia intracellularis in pig faeces that causes reduced average daily weight gains in experimentally challenged pigs.
abstract: Serology indicates that Lawsonia intracellularis infection is widespread in many countries, with most pigs seroconverting before 22 weeks of age. However, the majority of animals appear to be sub-clinically affected, demonstrated by the low reported prevalence of diarrhoea. Production losses caused by sub-clinical proliferative enteropathy (PE) are more difficult to diagnose, indicating the need for a quantitative L. intracellularis assay that correlates well with disease severity. In previous studies, increasing numbers of L. intracellularis in pig faeces, quantified with a real time polymerase chain reaction (qPCR), showed a strong negative correlation with average daily gain (ADG). In this study, the association between faecal L. intracellularis numbers and PE severity was examined in two L. intracellularis experimental challenge trials (n1=32 and n2=95). The number of L. intracellularis shed in individual faeces was determined by qPCR on days 0, 7, 14, 17 and 21 days post challenge, and average daily gain was recorded over the same period. The severity of histopathological lesions of PE was scored at 21 days post challenge. L. intracellularis numbers correlated well with histopathology severity and faecal consistency scores (r=0.72 and 0.68, respectively), and negatively with ADG (r=-0.44). Large reductions in ADG (131 g/day) occurred when the number of L. intracellularis shed by experimentally challenged pigs increased from 10(7) to 10(8)L. intracellularis, although smaller ADG reductions were also observed (15 g/day) when the number of L. intracellularis increased from 10(6) to 10(7)L. intracellularis.
authors.affiliation: New South Wales Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, PMB 4008, Narellan 2567, Australia. Electronic address: alison.collins@dpi.nsw.gov.au.
authors.forename: Alison M
authors.initials: AM
authors.lastname: Collins
authors.affiliation: New South Wales Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, PMB 4008, Narellan 2567, Australia.
authors.forename: Idris M
authors.initials: IM
authors.lastname: Barchia
mesh_terms: D000818:Animals; D045824:Desulfovibrionaceae Infections / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D003967:Diarrhea / Q000382:microbiology / Q000473:pathology / Q000662:veterinary; D005243:Feces / Q000382:microbiology; D005260:Female; D007410:Intestinal Diseases / Q000382:microbiology / Q000473:pathology / Q000662:veterinary; D020639:Lawsonia Bacteria / Q000235:genetics / Q000254:growth & development*; D008297:Male; D016133:Polymerase Chain Reaction; D012720:Severity of Illness Index; D034421:Sus scrofa / Q000254:growth & development / Q000382:microbiology; D013552:Swine / Q000254:growth & development / Q000382:microbiology; D013553:Swine Diseases / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D015430:Weight Gain
keywords: Average daily gain; Critical threshold; Lawsonia intracellularis; Proliferative enteropathy; Quantitative PCR