title: A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.
abstract: In order to apply human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) to regenerative medicine, the cells should be produced under restricted conditions conforming to GMP guidelines. Since the conventional culture system has some issues that need to be addressed to achieve this goal, we developed a novel culture system. We found that recombinant laminin-511 E8 fragments are useful matrices for maintaining hESCs and hiPSCs when used in combination with a completely xeno-free (Xf) medium, StemFit™. Using this system, hESCs and hiPSCs can be easily and stably passaged by dissociating the cells into single cells for long periods, without any karyotype abnormalities. Human iPSCs could be generated under feeder-free (Ff) and Xf culture systems from human primary fibroblasts and blood cells, and they possessed differentiation abilities. These results indicate that hiPSCs can be generated and maintained under this novel Ff and Xf culture system.
authors.affiliation: Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Nakagawa
authors.affiliation: Institute for Protein Research, Osaka University, Osaka 565-0871, Japan.
authors.forename: Yukimasa
authors.initials: Y
authors.lastname: Taniguchi
authors.affiliation: Institute for Innovation, Ajinomoto CO., Inc., Kawasaki, 210-8681 Japan.
authors.forename: Sho
authors.initials: S
authors.lastname: Senda
authors.affiliation: Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.
authors.forename: Nanako
authors.initials: N
authors.lastname: Takizawa
authors.affiliation: Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Ichisaka
authors.affiliation: Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Asano
authors.affiliation: Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.
authors.forename: Asuka
authors.initials: A
authors.lastname: Morizane
authors.affiliation: Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Doi
authors.affiliation: Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Takahashi
authors.affiliation: Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Nishizawa
authors.affiliation: Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Yoshida
authors.affiliation: Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.
authors.forename: Taro
authors.initials: T
authors.lastname: Toyoda
authors.affiliation: Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Osafune
authors.affiliation: Institute for Protein Research, Osaka University, Osaka 565-0871, Japan.
authors.forename: Kiyotoshi
authors.initials: K
authors.lastname: Sekiguchi
authors.affiliation: 1] Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan [2] Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158 USA.
authors.forename: Shinya
authors.initials: S
authors.lastname: Yamanaka
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D003470:Culture Media; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D012692:Serial Passage
chemical_list: D003470:Culture Media