title: Construction of novel Saccharomyces cerevisiae strains for bioethanol active dry yeast (ADY) production.
abstract: The application of active dry yeast (ADY) in bioethanol production simplifies operation processes and reduces the risk of bacterial contamination. In the present study, we constructed a novel ADY strain with improved stress tolerance and ethanol fermentation performances under stressful conditions. The industrial Saccharomyces cerevisiae strain ZTW1 showed excellent properties and thus subjected to a modified whole-genome shuffling (WGS) process to improve its ethanol titer, proliferation capability, and multiple stress tolerance for ADY production. The best-performing mutant, Z3-86, was obtained after three rounds of WGS, producing 4.4% more ethanol and retaining 2.15-fold higher viability than ZTW1 after drying. Proteomics and physiological analyses indicated that the altered expression patterns of genes involved in protein metabolism, plasma membrane composition, trehalose metabolism, and oxidative responses contribute to the trait improvement of Z3-86. This work not only successfully developed a novel S. cerevisiae mutant for application in commercial bioethanol production, but also enriched the current understanding of how WGS improves the complex traits of microbes.
authors.affiliation: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors.forename: Daoqiong
authors.initials: D
authors.lastname: Zheng
authors.affiliation: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors.forename: Kehui
authors.initials: K
authors.lastname: Gao
authors.affiliation: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors.forename: Zewei
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors.forename: Ou
authors.initials: O
authors.lastname: Li
authors.affiliation: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Motor Vehicle Biofuel Technology (Tianguan Group Co., Ltd.), Nanyang, Henan Province, China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Motor Vehicle Biofuel Technology (Tianguan Group Co., Ltd.), Nanyang, Henan Province, China.
authors.forename: Fengguang
authors.initials: F
authors.lastname: Du
authors.affiliation: State Key Laboratory of Motor Vehicle Biofuel Technology (Tianguan Group Co., Ltd.), Nanyang, Henan Province, China.
authors.forename: Peiyong
authors.initials: P
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Motor Vehicle Biofuel Technology (Tianguan Group Co., Ltd.), Nanyang, Henan Province, China.
authors.forename: Aimin
authors.initials: A
authors.lastname: Qu
authors.affiliation: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors.forename: Xuechang
authors.initials: X
authors.lastname: Wu
mesh_terms: D056804:Biofuels; D003681:Dehydration; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000235:genetics / Q000502:physiology; D007218:Industrial Microbiology / Q000379:methods*; D040901:Proteomics; D060888:Real-Time Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D013045:Species Specificity; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D056804:Biofuels; D000431:Ethanol