title: Three pentacyclic triterpenes protect H9c2 cardiomyoblast cells against high-glucose-induced injury.
abstract: H9c2 cardiomyoblast cell line was used to examine the protection of three triterpenes, asiatic acid, boswellic acid, and oleanolic acid, at 5 or 10 μM against high-glucose-induced injury. High glucose stimulated reactive oxygen species (ROS), oxidized glutathione (GSSG), interleukin-6, tumor necrosis factor-alpha, and monocyte chemoattractant protein-1 production, as well as decreased glutathione peroxidase (GPX), glutathione reductase (GR) and catalase activities, and protein expression. However, pre-treatments of three triterpenes reserved glutathione, maintained activity and expression of GPX, GR, and catalase, as well as lowered ROS, GSSG, and inflammatory cytokines generation. High glucose reduced Na(+)-K(+)-ATPase activity, raised nuclear factor kappa (NF-κ) B and caspase-3 activities, up-regulated protein expression of NF-κB, mitogen-activated protein kinase, Bax, and cleaved caspase-3, as well as down-regulated Bcl-2 expression. Pre-treatments of three triterpenes retained Na(+)-K(+)-ATPase activity, declined NF-κB and caspase-3 activities, reserved Bcl-2 expression, as well as suppressed protein expression of NF-κB, p-p38, Bax, and cleaved caspase-3. These findings suggest that these triterpenes are potent cardiac-protective agents.
authors.affiliation: Department of Nutrition, China Medical University , Taichung City , Taiwan.
authors.forename: C Y
authors.initials: CY
authors.lastname: Chan
authors.forename: M C
authors.initials: MC
authors.lastname: Mong
authors.forename: W H
authors.initials: WH
authors.lastname: Liu
authors.forename: C Y
authors.initials: CY
authors.lastname: Huang
authors.forename: M C
authors.initials: MC
authors.lastname: Yin
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism*; D018929:Cell Culture Techniques; D015536:Down-Regulation; D005947:Glucose / Q000009:adverse effects*; D006801:Humans; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D053978:Pentacyclic Triterpenes / Q000378:metabolism*; D017382:Reactive Oxygen Species; D014315:Triterpenes / Q000378:metabolism*
chemical_list: D053978:Pentacyclic Triterpenes; D017382:Reactive Oxygen Species; D014315:Triterpenes; C054625:boswellic acid; C017032:asiatic acid; D053148:Caspase 3; D005947:Glucose