title: Engineered micro-objects as scaffolding elements in cellular building blocks for bottom-up tissue engineering approaches.
abstract: A material-based bottom-up approach is proposed towards an assembly of cells and engineered micro-objects at the macroscale. We show how shape, size and wettability of engineered micro-objects play an important role in the behavior of cells on these objects. This approach can, among other applications, be used as a tool to engineer complex 3D tissues of clinically relevant size.
authors.affiliation: Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, 7522, NB, Enschede, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: Leferink
authors.forename: D
authors.initials: D
authors.lastname: Schipper
authors.forename: E
authors.initials: E
authors.lastname: Arts
authors.forename: E
authors.initials: E
authors.lastname: Vrij
authors.forename: N
authors.initials: N
authors.lastname: Rivron
authors.forename: M
authors.initials: M
authors.lastname: Karperien
authors.forename: K
authors.initials: K
authors.lastname: Mittmann
authors.forename: C
authors.initials: C
authors.lastname: van Blitterswijk
authors.forename: L
authors.initials: L
authors.lastname: Moroni
authors.forename: R
authors.initials: R
authors.lastname: Truckenmüller
mesh_terms: D000818:Animals; D002449:Cell Aggregation; D002460:Cell Line; D002470:Cell Survival; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D055616:Microtechnology / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
keywords: biofabrication; bottom-up tissue engineering; cell aggregates; human mesenchymal stromal cells; micro-objects