title: Lipase member H is a novel secreted protein selectively upregulated in human lung adenocarcinomas and bronchioloalveolar carcinomas.
abstract: Lung cancer is one of the most frequent causes of cancer-related death worldwide. However, molecular markers for lung cancer have not been well established. To identify novel genes related to lung cancer development, we surveyed publicly available DNA microarray data on lung cancer tissues. We identified lipase member H (LIPH, also known as mPA-PLA1) as one of the significantly upregulated genes in lung adenocarcinoma. LIPH was expressed in several adenocarcinoma cell lines when they were analyzed by quantitative real-time polymerase chain reaction (qPCR), western blotting, and sandwich enzyme-linked immunosorbent assay (ELISA). Immunohistochemical analysis detected LIPH expression in most of the adenocarcinomas and bronchioloalveolar carcinomas tissue sections obtained from lung cancer patients. LIPH expression was also observed less frequently in the squamous lung cancer tissue samples. Furthermore, LIPH protein was upregulated in the serum of early- and late-phase lung cancer patients when they were analyzed by ELISA. Interestingly, high serum level of LIPH was correlated with better survival in early phase lung cancer patients after surgery. Thus, LIPH may be a novel molecular biomarker for lung cancer, especially for adenocarcinoma and bronchioloalveolar carcinoma.
authors.affiliation: Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan; Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Seki
authors.affiliation: Department of Surgery, Asahi General Hospital, Chiba, Japan; Department of Thoracic Surgery, The University of Tokyo, Graduate School of Medicine, Tokyo, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Yoshida
authors.affiliation: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan; Graduate School of Life and Environmental Sciences, The University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Hisako
authors.initials: H
authors.lastname: Ishimine
authors.affiliation: Department of Pathology, Graduate School of Medicine, The University of Tokyo, Hongo, Tokyo, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Shinozaki-Ushiku
authors.affiliation: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Ito
authors.affiliation: Department of Internal Medicine, JA Kochi Hospital, Kochi, Japan.
authors.forename: Kenya
authors.initials: K
authors.lastname: Sumitomo
authors.affiliation: Department of Thoracic Surgery, The University of Tokyo, Graduate School of Medicine, Tokyo, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Nakajima
authors.affiliation: Department of Pathology, Graduate School of Medicine, The University of Tokyo, Hongo, Tokyo, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Fukayama
authors.affiliation: Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Michiue
authors.affiliation: Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan; Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan; Life Science Center of Tsukuba Advanced Research Alliance (TARA), The University of Tsukuba, Tsukuba, Ibaraki, Japan. Electronic address: asashi@bio.c.u-tokyo.ac.jp.
authors.forename: Makoto
authors.initials: M
authors.lastname: Asashima
authors.affiliation: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan; Graduate School of Life and Environmental Sciences, The University of Tsukuba, Tsukuba, Ibaraki, Japan. Electronic address: akikuri@hotmail.com.
authors.forename: Akira
authors.initials: A
authors.lastname: Kurisaki
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000235:genetics* / Q000378:metabolism*; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics* / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism; D018288:Carcinoma, Small Cell / Q000235:genetics / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D008049:Lipase / Q000097:blood / Q000235:genetics* / Q000378:metabolism*; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C504173:LIPC protein, human; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D008049:Lipase
keywords: Biomarker; Bronchioloalveolar carcinoma; Cell proliferation; Lipase member H; Lung adenocarcinoma; Prognostic marker