title: Effect of recombinant human MG53 protein on tourniquet-induced ischemia-reperfusion injury in rat muscle.
abstract: BACKGROUND
  Skeletal muscle ischemia-reperfusion injury (I-R) is a complex injury process that includes damage to the sarcolemmal membrane, contributing to necrosis and apoptosis. MG53, a muscle-specific TRIM family protein, has been shown to be essential for regulating membrane repair and has been shown to be protective against cardiac I-R and various forms of skeletal muscle injury. The purpose of this study was to determine if recombinant human MG53 (rhMG53) administration offered protection against I-R.
  
  
  METHODS
  rhMG53 was administered to rats immediately before tourniquet-induced ischemia and again immediately before reperfusion. Two days later muscle damage was assessed histologically.
  
  
  RESULTS
  rhMG53 offered no protective effect, as evidenced primarily by similar Evans blue dye inclusion in the muscles of rats administered rhMG53 or saline.
  
  
  CONCLUSIONS
  Administration of rhMG53 does not offer protection against I-R in rat skeletal muscle. Additional studies are required to determine if the lack of a response is species-specific.
authors.affiliation: United States Army Institute of Surgical Research, Extremity Trauma and Regenerative Medicine, 3698 Chambers Pass, Fort Sam Houston, Texas, 78234.
authors.forename: Benjamin T
authors.initials: BT
authors.lastname: Corona
authors.forename: Koyal
authors.initials: K
authors.lastname: Garg
authors.forename: Janet L
authors.initials: JL
authors.lastname: Roe
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.forename: Ki Ho
authors.initials: KH
authors.lastname: Park
authors.forename: Jianjie
authors.initials: J
authors.lastname: Ma
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Walters
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002352:Carrier Proteins / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D018482:Muscle, Skeletal / Q000098:blood supply / Q000293:injuries* / Q000473:pathology; D009336:Necrosis / Q000517:prevention & control; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D015427:Reperfusion Injury / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D014111:Tourniquets / Q000009:adverse effects*; D016896:Treatment Outcome; D000070584:Tripartite Motif Proteins
chemical_list: D002352:Carrier Proteins; D011994:Recombinant Proteins; C558399:TRIM72 protein, human; D000070584:Tripartite Motif Proteins
keywords: MG53; TRIM72; ischemia-reperfusion; muscle injury; tourniquet