title: Next-generation sequencing applied to flower development: ChIP-Seq.
abstract: Over the past 20 years, classic genetic approaches have shown that the developmental program underlying flower formation involves a large number of transcriptional regulators. However, the target genes of these transcription factors, as well as the gene regulatory networks they control, remain largely unknown. Chromatin immunoprecipitation coupled to next-generation sequencing (ChIP-Seq), which allows the identification of transcription factor binding sites on a genome-wide scale, has been successfully applied to a number of transcription factors in Arabidopsis. The ChIP-Seq procedure involves chemical cross-linking of proteins to DNA, followed by chromatin fragmentation and immunoprecipitation of specific protein-DNA complexes. The regions of the genome bound by a specific transcription factor can then be identified after next-generation sequencing.
authors.affiliation: Smurfit Institute of Genetics, Trinity College, Dublin, Ireland, graciete@tcd.ie.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Graciet
authors.forename: Diarmuid Seosamh
authors.initials: DS
authors.lastname: O'Maoiléidigh
authors.forename: Frank
authors.initials: F
authors.lastname: Wellmer
mesh_terms: D002843:Chromatin / Q000235:genetics; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D035264:Flowers / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods*; D016707:Tissue Fixation
chemical_list: D002843:Chromatin; D018744:DNA, Plant