title: Portable paper-based device for quantitative colorimetric assays relying on light reflectance principle.
abstract: This paper presents a novel paper-based analytical device based on the colorimetric paper assays through its light reflectance. The device is portable, low cost (<20 dollars), and lightweight (only 176 g) that is available to assess the cost-effectiveness and appropriateness of the original health care or on-site detection information. Based on the light reflectance principle, the signal can be obtained directly, stably and user-friendly in our device. We demonstrated the utility and broad applicability of this technique with measurements of different biological and pollution target samples (BSA, glucose, Fe, and nitrite). Moreover, the real samples of Fe (II) and nitrite in the local tap water were successfully analyzed, and compared with the standard UV absorption method, the quantitative results showed good performance, reproducibility, and reliability. This device could provide quantitative information very conveniently and show great potential to broad fields of resource-limited analysis, medical diagnostics, and on-site environmental detection.
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, P. R. China.
authors.forename: Bowei
authors.initials: B
authors.lastname: Li
authors.forename: Longwen
authors.initials: L
authors.lastname: Fu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Weiwei
authors.initials: W
authors.lastname: Feng
authors.forename: Lingxin
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D002417:Cattle; D003124:Colorimetry / Q000191:economics / Q000295:instrumentation*; D004867:Equipment Design; D005296:Ferrous Compounds / Q000032:analysis; D005947:Glucose / Q000032:analysis; D008027:Light; D057230:Limit of Detection; D009573:Nitrites / Q000032:analysis; D010209:Paper; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000032:analysis; D014867:Water / Q000032:analysis
chemical_list: D005296:Ferrous Compounds; D009573:Nitrites; D014867:Water; D012710:Serum Albumin, Bovine; D005947:Glucose
keywords: Colorimetric assay; Light reflectance; Metal ions; Nitrite; Paper-based microfluidic device; Point-of-care