title: Induction of caspase-9, biochemical assessment and morphological changes caused by apoptosis in cancer cells treated with goniothalamin extracted from Goniothalamus macrophyllus.
abstract: Goniothalamin, a natural compound extracted from Goniothalamus sp. belonging to the Annonacae family, possesses anticancer properties towards several tumor cell lines. This study focused on apoptosis induction by goniothalamin (GTN) in the Hela cervical cancer cell line. Cell growth inhibition was measured by MTT assay and the IC50 value of goniothalamin was 3.2 ± 0.72 μg/ml. Morphological changes and biochemical processes associated with apoptosis were evident on phase contrast microscopy and fluorescence microscopy. DNA fragmentation, DNA damage, caspase-9 activation and a large increase in the sub-G1 and S cell cycle phases confirmed the occurrence of apoptosis in a time-dependent manner. It could be concluded that goniothalamin show a promising cytotoxicity effect against cervical cancer cells (Hela) and the cell death mode induced by goniothalamin was apoptosis.
authors.affiliation: Dental Research and Training Unit, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia E-mail : aied_absi@yahoo.com.
authors.forename: Aied Mohammed
authors.initials: AM
authors.lastname: Alabsi
authors.forename: Rola
authors.initials: R
authors.lastname: Ali
authors.forename: Abdul Manaf
authors.initials: AM
authors.lastname: Ali
authors.forename: Hazlan
authors.initials: H
authors.lastname: Harun
authors.forename: Sami Abdo Radman
authors.initials: SA
authors.lastname: Al-Dubai
authors.forename: Kurubaran
authors.initials: K
authors.lastname: Ganasegeran
authors.forename: Mustafa Ahmed
authors.initials: MA
authors.lastname: Alshagga
authors.forename: Sameer Dirhim
authors.initials: SD
authors.lastname: Salem
authors.forename: Noor Hayaty Binti
authors.initials: NH
authors.lastname: Abu Kasim
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053453:Caspase 9 / Q000096:biosynthesis* / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D004249:DNA Damage / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004790:Enzyme Induction; D016193:G1 Phase / Q000187:drug effects; D054335:Goniothalamus / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011753:Pyrones / Q000737:chemistry* / Q000494:pharmacology*; D016196:S Phase / Q000187:drug effects
chemical_list: D010936:Plant Extracts; D011753:Pyrones; C103280:goniothalamin; D053453:Caspase 9