title: Subtractive screen of potential limb regeneration related genes from Pachytriton brevipes.
abstract: Regeneration capacity varies greatly among different animal species. In vertebrate, amphibian especially the Urodela, has been used as a powerful model system to study the mechanism of tissue regeneration because of the strong ability to regenerate their damaged or lost appendages. Pachytriton brevipes, a species of newt, which is widely distributed in south of China, can completely restore their damaged limbs within several months. In this study, we use modified suppression subtractive hybridization assay and dot-blot screening to identify candidate genes involved in tissue regeneration in P. brevipes. We successfully isolated 81 ESTs from a forward regeneration subtraction library. And we further verified the differential expression of four candidate genes, Rpl11, Cirbp, Ag2 and Trimx, between regenerating blastema and non-regeneration tissues by in situ hybridization. These genes were also be further characterized by phylogenetic and bioinformatic analysis. In general, we provided a comparative experimental approach to study the mechanisms of vertebrate regeneration.
authors.affiliation: Institute of Cellular and Developmental Biology, College of Life Sciences, Zhejiang University, 866# Yu Hang Tang Road, Hangzhou, 310058, Zhejiang, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Jiang
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Zhu
authors.forename: Jun-Fang
authors.initials: JF
authors.lastname: Zhao
authors.forename: Yan-Kuan
authors.initials: YK
authors.lastname: Zhou
authors.forename: Chao
authors.initials: C
authors.lastname: Zhong
authors.forename: Ji
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiao
authors.initials: X
authors.lastname: Huang
mesh_terms: D000818:Animals; D002681:China; D003001:Cloning, Molecular; D020224:Expressed Sequence Tags; D005121:Extremities / Q000254:growth & development* / Q000473:pathology; D010802:Phylogeny; D012038:Regeneration; D012455:Salamandridae / Q000254:growth & development*