title: Cloning and characterization of a novel vacuolar Na+/H+ antiporter gene (Dgnhx1) from chrysanthemum.
abstract: Plant vacuolar Na(+)/H(+) antiporter genes play significant roles in salt tolerance. However, the roles of the chrysanthemum vacuolar Na(+)/H(+) antiporter genes in salt stress response remain obscure. In this study, we isolated and characterized a novel vacuolar Na(+)/H(+) antiporter gene DgNHX1 from chrysanthemum. The DgNHX1 sequence contained 1920 bp with a complete open reading frame of 1533 bp encoding a putative protein of 510 amino acids with a predicted protein molecular weight of 56.3 kDa. DgNHX1 was predicted containing nine transmembrane domains. Its expression in the chrysanthemum was up-regulated by salt stress, but not by abscisic acid (ABA). To assess roles of DgNHX1 in plant salt stress responses, we performed gain-of-function experiment. The DgNHX1-overexpression tobacco plants showed significant salt tolerance than the wild type (WT). The transgenic lines exhibited more accumulation of Na(+) and K(+) under salt stress. These findings suggest that DgNHX1 plays a positive regulatory role in salt stress response.
authors.affiliation: Department of Ornamental Horticulture, Sichuan Agricultural University, Chengdu, China.
authors.forename: Qing-Lin
authors.initials: QL
authors.lastname: Liu
authors.affiliation: Key Lab of Plant Genetics & Molecular Breeding of Department of Life Science, Zhoukou Normal University, Zhoukou, China.
authors.forename: Ke-Dong
authors.initials: KD
authors.lastname: Xu
authors.affiliation: Department of Ornamental Horticulture, Sichuan Agricultural University, Chengdu, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhong
authors.affiliation: Department of Ornamental Horticulture, Sichuan Agricultural University, Chengdu, China.
authors.forename: Yuan-Zhi
authors.initials: YZ
authors.lastname: Pan
authors.affiliation: Department of Ornamental Horticulture, Sichuan Agricultural University, Chengdu, China.
authors.forename: Bei-Bei
authors.initials: BB
authors.lastname: Jiang
authors.affiliation: Department of Ornamental Horticulture, Sichuan Agricultural University, Chengdu, China.
authors.forename: Guang-Li
authors.initials: GL
authors.lastname: Liu
authors.affiliation: Department of Ornamental Horticulture, Sichuan Agricultural University, Chengdu, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Jia
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D031202:Chrysanthemum / Q000166:cytology* / Q000235:genetics*; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D012492:Salts / Q000494:pharmacology; D017421:Sequence Analysis; D012964:Sodium / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D014617:Vacuoles / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D012492:Salts; D017923:Sodium-Hydrogen Exchangers; D012964:Sodium; D011188:Potassium