title: [Relationship between the anticoagulant activity of sulfated plant polysaccharides and the area of their precipitation with polycations during biospecific electrophoresis].
abstract: Polyanions (in an amount within 1.5 - 6.0 mg), including cellulose sulfates (excreted from Gossipium hirsutum L., molecular weight 22.0 kDa, degree of sulfation within 0.8 - 1.8), inulin sulfates (excreted from Helianthus tuberosus, molecular weight 8.0 kDa, degree of sulfation within 0.6 - 1.6), pectin sulfates (excreted from Abies sibirica L., molecular weight 24.0 kDa, degree of sulfation within 0.8 - 1.1), give rise to peaks of precipitation with polycations of protamine sulfate. Only cellulose sulfates (in amount within 0.38 - 6.00 mg) give the peaks of precipitation with chitosan polycations (molecular weight 10 kDa, degree of deacetylation 85%) during horizontal biospecific electrophoresis. The height of the peak of precipitation with protamin sulfate was found to grow with increasing antithrombin activity of cellulose sulfates and pectin sulfate (for polyanions in an amount within 1.5 - 6 mg). The size of the area of precipitation with chitosan was found to decrease with increasing antithrombin activity of cellulose sulfates.
authors.forename: A A
authors.initials: AA
authors.lastname: Kuzhim
authors.forename: N N
authors.initials: NN
authors.lastname: Drozd
authors.forename: M A
authors.initials: MA
authors.lastname: Torlopov
authors.forename: A V
authors.initials: AV
authors.lastname: Il'ina
mesh_terms: D000925:Anticoagulants / Q000737:chemistry* / Q000302:isolation & purification; D001777:Blood Coagulation; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel / Q000379:methods; D003368:Gossypium / Q000737:chemistry; D006801:Humans; D007444:Inulin / Q000031:analogs & derivatives / Q000737:chemistry* / Q000302:isolation & purification; D008970:Molecular Weight; D010368:Pectins / Q000737:chemistry* / Q000302:isolation & purification; D028223:Pinus / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry* / Q000302:isolation & purification; D000071228:Polyelectrolytes; D011479:Protamines / Q000737:chemistry*; D013917:Thrombin / Q000737:chemistry
chemical_list: D000925:Anticoagulants; D002482:Cellulose; D002809:Chondroitin Sulfates; D007444:Inulin; D010368:Pectins; D011073:Polyamines; D000071228:Polyelectrolytes; D011479:Protamines; D013917:Thrombin; C020254:cellulose sulfate