title: Cloning and analysis of bile salt hydrolase genes from Lactobacillus plantarum CGMCC No. 8198.
abstract: Genes coding for bile salt hydrolase of Lactobacillus plantarum CGMCC 8198, a novel probiotic strain isolated from silage, were identified, analyzed and cloned. L. plantarum strongly resisted the inhibitory effects of bile salts and also decreased serum cholesterol levels by 20% in mice with hypercholesterolemia. Using RT-PCR analysis, bsh2, bsh3 and bsh4 were upregulated by bile salts in a dose-dependent manner. All three bsh genes had high similarity with those of other Lactobacillus strains. All three recombinant BSHs had high activities for the hydrolysis of glycodeoxycholic acids and taurodeoxycholic acids.
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Street, Tianjin Economic and Technological Development Area, Tianjin, 300457, China.
authors.forename: Xiang-Chao
authors.initials: XC
authors.lastname: Gu
authors.forename: Xue-Gang
authors.initials: XG
authors.lastname: Luo
authors.forename: Chong-Xi
authors.initials: CX
authors.lastname: Wang
authors.forename: De-Yun
authors.initials: DY
authors.lastname: Ma
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: He
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.forename: Tong-Cun
authors.initials: TC
authors.lastname: Zhang
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D000924:Anticholesteremic Agents / Q000737:chemistry / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002793:Cholic Acids / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D006937:Hypercholesterolemia / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D017421:Sequence Analysis
chemical_list: D000924:Anticholesteremic Agents; D001426:Bacterial Proteins; D002793:Cholic Acids; D000581:Amidohydrolases; C023154:choloylglycine hydrolase