title: Age-associated alterations in the time-dependent profile of pro- and anti-inflammatory proteins within the hippocampus in response to acute exposure to interleukin-1β.
abstract: The pro-inflammatory cytokine IL-1β is known to play a role in several models of aging, neuroinflammation, and neurodegenerative diseases. Here, we document a detailed time- and age-dependent pattern of pro- and anti-inflammatory biomarkers following bilateral intrahippocampal injection of interleukin-1β. During the first 12h several pro- and anti-inflammatory cytokines increased in the aged (24 mo old) rats, some of which returned to baseline levels by 24h post-injection while others remained elevated for 72 h post-injection. In contrast, no such increases were observed in the young (3 mo old) rats. Interestingly, young rats up-regulated mRNA of two pro-inflammatory cytokines, interleukin-1β and tumor necrosis factor-α, but did not translate these transcripts into functional proteins, which may be related to expression of suppressor of cytokine signaling type-2. These results contribute to our understanding of how neuroinflammation may contribute to the pathogenesis of age-related neurodegenerative disorders due to an age-related bias towards a hyper-reactive immune response that is not selective for a pro- or anti-inflammatory phenotype following an inflammatory stimulus.
authors.affiliation: Department of Neuroscience, Ohio State University, Columbus, OH 43210, USA.
authors.forename: Sarah C
authors.initials: SC
authors.lastname: Hopp
authors.affiliation: Department of Neuroscience, Ohio State University, Columbus, OH 43210, USA.
authors.forename: Sarah
authors.initials: S
authors.lastname: Royer
authors.affiliation: Department of Psychology, Ohio State University, Columbus, OH 43210, USA.
authors.forename: Holly M
authors.initials: HM
authors.lastname: Brothers
authors.affiliation: Department of Psychology, Ohio State University, Columbus, OH 43210, USA.
authors.forename: Roxanne M
authors.initials: RM
authors.lastname: Kaercher
authors.affiliation: Department of Neuroscience, Ohio State University, Columbus, OH 43210, USA.
authors.forename: Heather
authors.initials: H
authors.lastname: D'Angelo
authors.affiliation: Department of Psychology, Ohio State University, Columbus, OH 43210, USA.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Bardou
authors.affiliation: Department of Psychology, Ohio State University, Columbus, OH 43210, USA. Electronic address: wenk.6@osu.edu.
authors.forename: Gary L
authors.initials: GL
authors.lastname: Wenk
mesh_terms: D000375:Aging; D000704:Analysis of Variance; D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects*; D053583:Interleukin-1beta / Q000494:pharmacology*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D054367:Receptors, CXCR3 / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism
chemical_list: C515189:Cxcr3 protein, rat; D016207:Cytokines; D053583:Interleukin-1beta; D012333:RNA, Messenger; D054367:Receptors, CXCR3; C491408:Socs2 protein, rat; D050826:Suppressor of Cytokine Signaling Proteins; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Aging; Cytokines; Hippocampus; Microglia; Neuroinflammation; Rat