title: Three dimensional microfluidics with embedded microball lenses for parallel and high throughput multicolor fluorescence detection.
abstract: We report a 3D microfluidic device with 32 detection channels and 64 sheath flow channels and embedded microball lens array for high throughput multicolor fluorescence detection. A throughput of 358 400 cells/s has been accomplished. This device is realized by utilizing solid immersion micro ball lens arrays for high sensitivity and parallel fluorescence detection. High refractive index micro ball lenses (n = 2.1) are embedded underneath PDMS channels close to cell detection zones in channels. This design permits patterning high N.A. micro ball lenses in a compact fashion for parallel fluorescence detection on a small footprint device. This device also utilizes 3D microfluidic fabrication to address fluid routing issues in two-dimensional parallel sheath focusing and allows simultaneous pumping of 32 sample channels and 64 sheath flow channels with only two inlets.
authors.affiliation: Mechanical and Aerospace Engineering Department, University of California Los Angeles, Los Angeles, California 90095, USA ; Institute of Applied Mechanics, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Fan
authors.affiliation: Mechanical and Aerospace Engineering Department, University of California Los Angeles, Los Angeles, California 90095, USA.
authors.forename: Y C
authors.initials: YC
authors.lastname: Wu
authors.affiliation: Mechanical and Aerospace Engineering Department, University of California Los Angeles, Los Angeles, California 90095, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Mechanical and Aerospace Engineering Department, University of California Los Angeles, Los Angeles, California 90095, USA.
authors.forename: Y C
authors.initials: YC
authors.lastname: Kung
authors.affiliation: Mechanical and Aerospace Engineering Department, University of California Los Angeles, Los Angeles, California 90095, USA ; Department of Pathology and Laboratory Medicine, University of California Los Angeles, Los Angeles, California 90095, USA.
authors.forename: T H
authors.initials: TH
authors.lastname: Wu
authors.affiliation: Mechanical and Aerospace Engineering Department, University of California Los Angeles, Los Angeles, California 90095, USA.
authors.forename: K W
authors.initials: KW
authors.lastname: Huang
authors.affiliation: Institute of Applied Mechanics, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: H J
authors.initials: HJ
authors.lastname: Sheen
authors.affiliation: Mechanical and Aerospace Engineering Department, University of California Los Angeles, Los Angeles, California 90095, USA ; Department of Bioengineering, University of California Los Angeles, Los Angeles, California 90095, USA.
authors.forename: P Y
authors.initials: PY
authors.lastname: Chiou