title: Inhibitory effects ofDendrobium candidumWall ex Lindl. on azoxymethane- and dextran sulfate sodium-induced colon carcinogenesis in C57BL/6 mice.
abstract: Dendrobium candidumWall ex Lindl. was purchased for the evaluation of azoxymethane (AOM)- and dextran sulfate sodium (DSS)-induced colon carcinogenesis in C57BL/6 mice. The body weights of the AOM- and DSS-induced colon cancer control groups were lighter than those of the untreated mice.D. candidumincreased the body weights of the mice compared with the control group, and reduced the levels of the serum proinflammatory cytokines, IL-6, IL-12, TNF-α and IFN-γ, compared with the colon cancer control group. Reverse transcription-polymerase chain reaction and western blot analyses of the apoptotic-related genes, bax, bcl-2, caspase-3 and caspase-9, were performed in the colon tissues. The high-concentrationD. candidumgroup showed a significant increase in the mRNA and protein expression levels of bax, caspase-3 and caspase-9 and decreased expression levels of bcl-2 compared with the control group. These results indicate thatD. candidumWall ex Lindl. exhibits preventive effects against colon carcinogenesis in mice.
authors.affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Sun
authors.affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China.
authors.forename: Guijie
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhu
authors.affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China.
authors.forename: Cun
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
keywords: C57BL/6 mice; Dendrobium candidum Wall ex Lindl.; colon carcinogenesis; cytokine