title: Copper alters the effect of temperature on mitochondrial bioenergetics in rainbow trout, Oncorhynchus mykiss.
abstract: We investigated the interaction of temperature and copper (Cu) on mitochondrial bioenergetics to gain insight into how temperature fluctuations imposed by natural phenomena or anthropogenic activities would modulate the effects of Cu on cellular energy homeostasis. Mitochondria were isolated from rainbow trout livers and, in the first set of experiments, exposed to Cu (0-2.5 mM) at 5, 11, and 25 °C with measurement of mitochondrial complex II (mtCII)-driven respiration. In the second set of experiments, unenergized mitochondria were incubated for 30 or 60 min with lower concentrations (0-160 μM) of Cu to measure the effects on mtCII enzyme activity. Whereas maximal (state 3) respiration was inhibited by high Cu exposure, low Cu doses stimulated and high Cu doses inhibited resting (state 4) and 4ol (proton leak) respirations. High temperature alone increased mitochondrial respiration in all states. The Q10 values for state 3, state 4, and proton leak respirations suggested active processes with state 4 respiration and proton leak exhibiting greater thermal sensitivity than state 3 respiration. The differential thermal sensitivity of resting relative to phosphorylating mitochondrial state led to uncoupling and limitation of mitochondrial oxidative capacity at both high temperature and doses of Cu. Moreover, exposure to high Cu caused loss of thermal dependence of the mitochondrial bioenergetics culminating in Q10 values well below unity and decreased activation energies (E a) for both maximal and resting respiration rates. In addition, mtCII activity was increased by low and decreased by high doses of Cu indicating that direct effects on this enzyme contribute to Cu-induced mitochondrial dysfunction. Taken together, it appears that the substrate oxidation (electron transport chain and tricarboxylic acid cycle) and proton leak subsystems are targets of the deleterious effects of Cu and increased temperature on mitochondrial bioenergetics. However, mitochondrial effects of Cu and temperature may not be easily distinguished because they are generally qualitatively similar.
authors.affiliation: Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, 550 University Ave., Charlottetown, PE, C1A 4P3, Canada.
authors.forename: Ravinder
authors.initials: R
authors.lastname: Sappal
authors.forename: Michelle
authors.initials: M
authors.lastname: Macdougald
authors.forename: Don
authors.initials: D
authors.lastname: Stevens
authors.forename: Mark D
authors.initials: MD
authors.lastname: Fast
authors.forename: Collins
authors.initials: C
authors.lastname: Kamunde
mesh_terms: D000818:Animals; D019069:Cell Respiration / Q000187:drug effects; D003300:Copper / Q000633:toxicity*; D004734:Energy Metabolism / Q000187:drug effects*; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D017686:Oncorhynchus mykiss / Q000502:physiology; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D003300:Copper