title: L-type Ca2+ channel blocker attenuates impairment induced by acidosis in hippocampal neurons.
abstract: Accumulation of acid in the brain during ischemia may contribute to the activation of voltage-gated Ca2+ channels and subsequent neuronal injury. However, information regarding the role of L-type Ca2+ channel under acidosis remains unclear. In the present study, we examined the role of L-type Ca2+ channel in acidosis induced neuronal death and subsequent pathogenesis events responsible for neuron degeneration. Here we report that preincubation of cells with nifedipine (5 microM, 10 microM), an inhibitor of L-type Ca2+ channel markedly reduced neuronal death induced by moderate extracellular acidosis (pH 6.5) on cultured hippocampus neurons. Furthermore, nifedipine decreased the hippocampus neuronal swelling, as well as the accumulation of Ca2+ and collapse of mitochondrial membrane potential induced by acidosis. These findings demonstrate that pharmacological inhibition of L-type Ca2+ channel would attenuate neuronal degeneration caused by toxic low pH exposure in rat hippocampus neuron.
authors.affiliation: Key Laboratory of Medicinal Chemistry for Natural Resources, Ministry of Education, School of Chemical Science and Technology, Yunnan University, Kunming, China. zeng-89@hotmail.com
authors.forename: Xianghui
authors.initials: X
authors.lastname: Zeng
authors.affiliation: School of Pharmaceutical Sciences, Kunming Medical University, Kunming, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Sciences, Kunming Medical University, Kunming, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Qing
authors.affiliation: Key Laboratory of Medicinal Chemistry for Natural Resources, Ministry of Education, School of Chemical Science and Technology, Yunnan University, Kunming, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000138:Acidosis / Q000188:drug therapy* / Q000473:pathology*; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D020746:Calcium Channels, L-Type / Q000187:drug effects*; D020013:Calcium Signaling / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D048429:Cell Size; D004232:Diuretics / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000473:pathology*; D009543:Nifedipine / Q000494:pharmacology; D061251:Primary Cell Culture; D051381:Rats
chemical_list: D002121:Calcium Channel Blockers; D020746:Calcium Channels, L-Type; D004232:Diuretics; D000584:Amiloride; D009543:Nifedipine