title: Cyclic dipeptides from lactic acid bacteria inhibit proliferation of the influenza A virus.
abstract: We isolated Lactobacillus plantarum LBP-K10 from the traditional Korean fermented food kimchi. When organic acids were removed, the culture filtrate of this isolate showed high antiviral activity (measured using a plaque-forming assay) against the influenza A (H3N2) virus. Two fractions that were active against influenza A virus were purified from the culture filtrate using a C18 column with high-performance liquid chromatography. These active fractions were crystallized and identified to be the cyclic dipeptides cis-cyclo (L-Leu-L-Pro) and cis-cyclo(L-Phe-L-Pro) using gas chromatography-mass spectrometry; this identification was confirmed by X-ray crystallography. These cyclic dipeptides were identified in the culture filtrate of other lactic acid bacteria, including Lactobacillus spp., Leuconostoc spp., Weissella spp., and Lactococcus lactis.
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul, 151-747, Republic of Korea.
authors.forename: Min-Kyu
authors.initials: MK
authors.lastname: Kwak
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Jun-Oh
authors.initials: JO
authors.lastname: Kwon
authors.forename: Min-Kyu
authors.initials: MK
authors.lastname: Kim
authors.forename: Andrew HyoungJin
authors.initials: AH
authors.lastname: Kim
authors.forename: Sa-Ouk
authors.initials: SO
authors.lastname: Kang
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004151:Dipeptides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D053122:Influenza A Virus, H3N2 Subtype / Q000187:drug effects* / Q000254:growth & development; D019344:Lactic Acid / Q000378:metabolism; D007778:Lactobacillus / Q000737:chemistry / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure
chemical_list: D000998:Antiviral Agents; D004151:Dipeptides; D019344:Lactic Acid