title: Translating ribosome affinity purification (TRAP) for cell-specific translation profiling in developing flowers.
abstract: The development of a multicellular organism is accompanied by cell differentiation. In fact, many biological processes have cell specificity, such that distinct cell types respond differently to endogenous or environmental cues. To obtain cell-specific gene expression profiles, translating ribosome affinity purification (TRAP) has been developed to label polysomes containing translating mRNAs in genetically defined cell types. Here, we describe the immunopurification of epitope-labeled polysomes and associated RNAs from target cell types. TRAP has the additional advantage of obtaining only translating mRNAs, which are a better proxy to the proteome than a standard mRNA preparation.
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuling
authors.initials: Y
authors.lastname: Jiao
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development; D035264:Flowers / Q000235:genetics* / Q000254:growth & development*; D047468:Immunoprecipitation / Q000379:methods*; D008863:Microspheres; D011132:Polyribosomes / Q000235:genetics; D014176:Protein Biosynthesis; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification; D012270:Ribosomes / Q000235:genetics*; D012685:Sepharose / Q000737:chemistry
chemical_list: D018749:RNA, Plant; D012685:Sepharose