title: Small noncoding RNAs in cells transformed by human T-cell leukemia virus type 1: a role for a tRNA fragment as a primer for reverse transcriptase.
abstract: The present study employed mass sequencing of small RNA libraries to identify the repertoire of small noncoding RNAs expressed in normal CD4(+) T cells compared to cells transformed with human T-cell leukemia virus type 1 (HTLV-1), the causative agent of adult T-cell leukemia/lymphoma (ATLL). The results revealed distinct patterns of microRNA expression in HTLV-1-infected CD4(+) T-cell lines with respect to their normal counterparts. In addition, a search for virus-encoded microRNAs yielded 2 sequences that originated from the plus strand of the HTLV-1 genome. Several sequences derived from tRNAs were expressed at substantial levels in both uninfected and infected cells. One of the most abundant tRNA fragments (tRF-3019) was derived from the 3' end of tRNA-proline. tRF-3019 exhibited perfect sequence complementarity to the primer binding site of HTLV-1. The results of an in vitro reverse transcriptase assay verified that tRF-3019 was capable of priming HTLV-1 reverse transcriptase. Both tRNA-proline and tRF-3019 were detected in virus particles isolated from HTLV-1-infected cells. These findings suggest that tRF-3019 may play an important role in priming HTLV-1 reverse transcription and could thus represent a novel target to control HTLV-1 infection.
  
  
  OBJECTIVE
  Small noncoding RNAs, a growing family of regulatory RNAs that includes microRNAs and tRNA fragments, have recently emerged as key players in many biological processes, including viral infection and cancer. In the present study, we employed mass sequencing to identify the repertoire of small noncoding RNAs in normal T cells compared to T cells transformed with human T-cell leukemia virus type 1 (HTLV-1), a retrovirus that causes adult T-cell leukemia/lymphoma. The results revealed a distinct pattern of microRNA expression in HTLV-1-infected cells and a tRNA fragment (tRF-3019) that was packaged into virions and capable of priming HTLV-1 reverse transcription, a key event in the retroviral life cycle. These findings indicate tRF-3019 could represent a novel target for therapies aimed at controlling HTLV-1 infection.
authors.affiliation: Department of Surgery, Oncology and Gastroenterology, University of Padua, Padua, Italy.
authors.forename: Katia
authors.initials: K
authors.lastname: Ruggero
authors.forename: Alessandro
authors.initials: A
authors.lastname: Guffanti
authors.forename: Alberto
authors.initials: A
authors.lastname: Corradin
authors.forename: Varun Kumar
authors.initials: VK
authors.lastname: Sharma
authors.forename: Gianluca
authors.initials: G
authors.lastname: De Bellis
authors.forename: Giorgio
authors.initials: G
authors.lastname: Corti
authors.forename: Angela
authors.initials: A
authors.lastname: Grassi
authors.forename: Paola
authors.initials: P
authors.lastname: Zanovello
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Bronte
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Ciminale
authors.forename: Donna M
authors.initials: DM
authors.lastname: D'Agostino
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000821:virology*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D054884:Host-Pathogen Interactions; D015368:Human T-lymphotropic virus 1 / Q000502:physiology*; D006801:Humans; D058727:RNA, Small Untranslated / Q000378:metabolism*; D012361:RNA, Transfer, Pro / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000096:biosynthesis / Q000378:metabolism*; D048348:Reverse Transcription
chemical_list: D058727:RNA, Small Untranslated; D012361:RNA, Transfer, Pro; D012194:RNA-Directed DNA Polymerase