title: Antimutagenic activity of ipriflavone against the DNA-damage induced by cyclophosphamide in mice.
abstract: In the present study we evaluated the potential of ipriflavone against the cytotoxic and mutagenic effects induced by cyclophosphamide chemotherapeutic agent in bone marrow cells of mice, using the micronucleus assay in vivo on cells of bone marrow. The study was performed following three protocols: pre-treatment, simultaneous treatment and post treatment. The results demonstrated that ipriflavone has a protective effect against mutagenicity induced by cyclophosphamide in the pre-treatment and post-treatment and against the cytotoxicity in all treatments. There was variation between the genders in some of the experimental groups. To evaluate their possible mechanisms of action, it was performed the DPPH assay, which showed no ability to donate hydrogens, suggesting that it acts through other mechanisms. Due to its ability to prevent chromosomal damage, ipriflavone is likely to open an interest field concerning its possible the use in clinical applications.
authors.affiliation: Departamento de Ciências Biológicas, Universidade Federal do Espírito Santo, Vitória, ES, Brazil. Electronic address: judelarmelina@yahoo.com.br.
authors.forename: Juliana Macedo
authors.initials: JM
authors.lastname: Delarmelina
authors.affiliation: Departamento de Ciências Biológicas, Universidade Federal do Espírito Santo, Vitória, ES, Brazil. Electronic address: jeanvencioneck@gmail.com.
authors.forename: Jean Carlos Vencioneck
authors.initials: JC
authors.lastname: Dutra
authors.affiliation: Departamento de Ciências Biológicas, Universidade Federal do Espírito Santo, Vitória, ES, Brazil. Electronic address: docarmo_batitucci@yahoo.com.br.
authors.forename: Maria do Carmo Pimentel
authors.initials: Mdo C
authors.lastname: Batitucci
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D003520:Cyclophosphamide / Q000633:toxicity*; D004249:DNA Damage; D005260:Female; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D051379:Mice
chemical_list: D016587:Antimutagenic Agents; D007529:Isoflavones; C018986:ipriflavone; D003520:Cyclophosphamide
keywords: Antimutagenicity; DPPH assay; Ipriflavone; Micronucleus assay