title: Roles of common subunits within distinct multisubunit complexes.
abstract: Currently, there is no method to distinguish between the roles of a subunit in one multisubunit protein complex from its roles in other complexes in vivo. This is because a mutation in a common subunit will affect all complexes containing that subunit. Here, we describe a unique method to discriminate between the functions of a common subunit in different multisubunit protein complexes. In this method, a common subunit in a multisubunit protein complex is genetically fused to a subunit that is specific to that complex and point mutated. The resulting phenotype(s) identify the specific function(s) of the subunit in that complex only. Histone H2B is a common subunit in nucleosomes containing H2A/H2B or Htz1/H2B dimers. The H2B was fused to H2A or Htz1 and point mutated. This strategy revealed that H2B has common and distinct functions in different nucleosomes. This method could be used to study common subunits in other multisubunit protein complexes.
authors.affiliation: Department of Biochemistry, Tohoku Pharmaceutical University, Sendai, Miyagi 981-8558, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Nakabayashi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kawashima
authors.forename: Takemi
authors.initials: T
authors.lastname: Enomoto
authors.forename: Masayuki
authors.initials: M
authors.lastname: Seki
authors.forename: Masami
authors.initials: M
authors.lastname: Horikoshi
mesh_terms: D015152:Blotting, Northern; D047369:Chromatin Immunoprecipitation; D006657:Histones / Q000235:genetics / Q000378:metabolism; D015151:Immunoblotting; D046912:Multiprotein Complexes / Q000235:genetics*; D009707:Nucleosomes / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D017354:Point Mutation / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D004718:Saccharomycetales / Q000235:genetics*; D016019:Survival Analysis
chemical_list: D006657:Histones; D046912:Multiprotein Complexes; D009707:Nucleosomes; D011506:Proteins
keywords: FALC; chromatin; epigenetics; histone variant; modification