title: Naringin mitigates erythrocytes aging induced by paclitaxel: an in vitro study.
abstract: In this study, the protective role of naringin (NAR) against paclitaxel (PTX)-induced erythrocytes aging has been investigated using human erythrocyte as an in vitro model. Erythrocytes were incubated with PTX in the presence and absence of NAR. Incubation of erythrocytes with PTX resulted in increased protein carbonyl content and malondialdehyde and hemolysis percentage compared with control. Furthermore, a significant increase in the ratios of glutathione peroxidase/glutathione reductase, superoxide dismutase/glutathione peroxidase, and superoxide dismutase/catalase in PTX-treated cells was observed, compared with control cells. In contrast, reduced glutathione/oxidized glutathione ratio and glucose-6-phosphate dehydrogenase activity were decreased upon PTX treatment. The simultaneous incubation of erythrocytes with PTX and NAR restored these variables to values similar to those of control erythrocytes. These results suggest that NAR inhibited PTX-induced aging by lessening the PTX-induced oxidative stress.
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia; Department of Biochemistry, College of Pharmacy, Al-Azhar University (Boys), Nasr City, Cairo, Egypt. gamal.harisa@yahoo.com.
authors.forename: Gamaleldin I
authors.initials: GI
authors.lastname: Harisa
mesh_terms: D000328:Adult; D002374:Catalase / Q000378:metabolism; D016922:Cellular Senescence / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D044950:Flavanones / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D017239:Paclitaxel / Q000009:adverse effects*; D050050:Protein Carbonylation; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D044950:Flavanones; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione; C005274:naringin; D017239:Paclitaxel
keywords: Aging; Erythrocytes; Naringin; Oxidative Stress; Paclitaxel