title: Surface plasma treatment of poly(caprolactone) micro, nano, and multiscale fibrous scaffolds for enhanced osteoconductivity.
abstract: In this study, poly(caprolactone) (PCL) was electrospun to nano, micro, and multiscale (micro-nano) fibers, which were then subjected to low pressure argon and nitrogen plasma treatment. The electrospun fibers contain microfibers of diameter 8-10 μm and nanofibers of diameter 200-300 nm. Characterization of the plasma-treated fibers showed that treatment using less oxidizing gas like nitrogen and inert gas like argon functionalize the surface with polar groups that significantly modify the properties of the scaffold. Highly hydrophobic PCL fibrous scaffolds were rendered hydrophilic, with significantly improved biomineralization after the plasma treatment. While plasma treatment on micro and multiscale fibers enhanced their protein adsorption, cell attachment, spreading, elongation, and proliferation, nanofibers showed remarkably improved cell attachment. The applicability of plasma-treated electrospun fibers for differentiation of mesenchymal stem cell toward osteogenic lineage was also studied. Accelerated differentiation toward osteoblast lineage, with maximum alkaline phosphatase (ALP) activity in 14 days was achieved in plasma-treated fibers. Another remarkable outcome was the enhanced ALP activity of the microfibers after plasma treatment, compared with multiscale and nanofibers. Alizarin red staining further confirmed the mineralization of the plasma-treated scaffolds, indicative of maturation of the differentiated cells. This work thus concentrates on harnessing the potential of plasma treatment, for improving the osteoconductivity of fibrous scaffolds, which could be used for bone tissue engineering/regenerative medicine.
authors.affiliation: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University , Kochi, India .
authors.forename: Deepthi
authors.initials: D
authors.lastname: Sankar
authors.forename: K T
authors.initials: KT
authors.lastname: Shalumon
authors.forename: K P
authors.initials: KP
authors.lastname: Chennazhi
authors.forename: Deepthy
authors.initials: D
authors.lastname: Menon
authors.forename: R
authors.initials: R
authors.lastname: Jayakumar
mesh_terms: D000327:Adsorption; D000469:Alkaline Phosphatase / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism; D001861:Bone Regeneration / Q000187:drug effects*; D002113:Calcification, Physiologic / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D058626:Plasma Gases / Q000494:pharmacology*; D011091:Polyesters / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013194:Staining and Labeling; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D001798:Blood Proteins; D058626:Plasma Gases; D011091:Polyesters; D014867:Water; C016240:polycaprolactone; D000469:Alkaline Phosphatase