title: [Effect of Solanum nigrum on human colon carcinoma RKO cells ].
abstract: OBJECTIVE
  To evaluate the effect of Solanum nigrum on adhesion, migration and invasion in human colon carcinoma RKO cells.
  
  
  METHODS
  RKO cells were treated with different dose of Solanum nigrum. Cell proliferation was detected by CCK-8 assay. Cell adhesion was observed with CytoSelect 48-Well Cell Adhesion Assay. Cell migration was detected with scratch assay. Cell invasion was analyzed by CytoSelect 24-Well Cell Invasion Assay.
  
  
  RESULTS
  At final concentration of 400-1600 microg/mL, Solanum nigrum significantly inhibited proliferation of RKO cells in a dose-dependent manner. At final concentration of 100-400 microg/mL, Solanum nigrum significantly inhibited adhesion,migration and invasion in RKO cells.
  
  
  CONCLUSIONS
  Solanum nigrum may inhibit the proliferation, adhesion, migration and invasive abilities in RKO cells. The present study provides new insight into the application of Solanum nigrum for colon carcinoma treatment that are worthy of further study.
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. beearhu@hotmail.com
authors.forename: Bing
authors.initials: B
authors.lastname: Hu
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: An
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
authors.forename: Ke-Ping
authors.initials: KP
authors.lastname: Shen
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
authors.forename: Xiu-Feng
authors.initials: XF
authors.lastname: Shi
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Deng
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
authors.forename: Meng-Meng
authors.initials: MM
authors.lastname: Wei
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000473:pathology*; D006801:Humans; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D032324:Solanum nigrum / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts