title: Interaction of surface molecules on Cryptococcus neoformans with plasminogen.
abstract: Microbial pathogens are known to express molecules that interact with host proteins, leading to invasion and colonization. For example, some pathogenic microorganisms express proteins that bind to and enhance the activity of plasminogen. In this way, pathogens utilize the host fibrinolytic system to promote invasion. We found that triosephosphate isomerase (TPI), a glycolytic enzyme produced by Staphylococcus aureus, bound to mannooligosaccharides from the pathogenic capsulated fungus Cryptococcus neoformans and human plasminogen, suggesting that TPI is a moonlighting protein. Several C. neoformans surface proteins are thought to be plasminogen-binding proteins. Here, we examined the ability of surface polymers (including polysaccharides) to bind plasminogen. Heat-killed C. neoformans cells transformed plasminogen into plasmin in a dose-dependent manner in the presence of tissue plasminogen activator. Soluble polysaccharides were found to bind plasminogen based on surface plasmon resonance (SPR) analysis. Neutral polysaccharides fractionated using DEAE column chromatography bound and activated plasminogen. However, the fraction containing glucuronoxylomannan (the primary component of the capsule) did not activate plasminogen. In addition, binding between glucuronoxylomannan and plasminogen was weak. Components of the neutral polysaccharides were identified as mannose, galactose, glucose and xylose. In conclusion, neutral polysaccharides that may affect fibrinolysis were detected on the surface of C. neoformans.
authors.affiliation: Department of Microbial Science and Host Defense, Meiji Pharmaceutical University, Noshio, Kiyose, Tokyo, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Ikeda
authors.forename: Tomoe
authors.initials: T
authors.lastname: Ichikawa
mesh_terms: D001711:Biotransformation; D003455:Cryptococcus neoformans / Q000502:physiology*; D005341:Fibrinolysin / Q000378:metabolism; D006801:Humans; D010958:Plasminogen / Q000378:metabolism*; D010960:Plasminogen Activators / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D011485:Protein Binding; D020349:Surface Plasmon Resonance
chemical_list: D011134:Polysaccharides; D010958:Plasminogen; D010960:Plasminogen Activators; D005341:Fibrinolysin
keywords: Cryptococcus neoformans; capsule; glucuronoxylomannan; plasminogen