title: The outer membrane protein, LamB (maltoporin), is a versatile vaccine candidate among the Vibrio species.
abstract: Maltoporin (LamB) is a family of outer membrane proteins. There has been no report of immunological characteristics of LamB in the Vibrio species so far. In this study, lamB genes from eight Vibrio strains were cloned and sequenced. The bioinformatics analysis indicated that sequence similarities of LamB proteins were ranged from 46.7% to 81.1%. Further, the result showed that their antigenic epitopes were highly conserved implying that LamB might be a shared antigen among Vibrios. The Western blot of rabbit sera against recombinant LamB from V. alginolyticus ATCC 33787 with cell lysate of 18 Vibrio strains showed cross-recognition. Bands observed on cell lysate of Vibrio strains immunoblotted with the anti-LamB sera ranged between 40 and 49 kDa. The Whole-cell ELISA assay further confirmed that the antisera of recombinant LamB recognized the tested Vibrio strains indicating the surface-exposed of LamB. Finally, the cross-protective property of recombinant LamB was evaluated through vaccination and subsequent challenge with heterogeneous virulent Vibrio strains in zebrafish. Recorded relative percent survival (RPS) of the vaccinated group varied from 54.1% to 77.8%, showing that zebrafish were protected from Vibrio infection after immunization with LamB protein. The cumulative evidences in this study suggested that LamB was a conserved antigen among tested Vibrio species and might be a potentially versatile vaccine candidate for the prevention of Vibriosis.
authors.affiliation: Department of Biology, School of Science, Shantou University, Shantou, Guangdong 515063, China.
authors.forename: Jingsheng
authors.initials: J
authors.lastname: Lun
authors.affiliation: Department of Biology, School of Science, Shantou University, Shantou, Guangdong 515063, China.
authors.forename: Changyan
authors.initials: C
authors.lastname: Xia
authors.affiliation: Department of Biology, School of Science, Shantou University, Shantou, Guangdong 515063, China.
authors.forename: Chuanfei
authors.initials: C
authors.lastname: Yuan
authors.affiliation: Department of Biology, School of Science, Shantou University, Shantou, Guangdong 515063, China.
authors.forename: Yueling
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Biology, School of Science, Shantou University, Shantou, Guangdong 515063, China.
authors.forename: Mingqi
authors.initials: M
authors.lastname: Zhong
authors.affiliation: Department of Biology, School of Science, Shantou University, Shantou, Guangdong 515063, China.
authors.forename: Tongwang
authors.initials: T
authors.lastname: Huang
authors.affiliation: Department of Biology, School of Science, Shantou University, Shantou, Guangdong 515063, China. Electronic address: hzh@stu.edu.cn.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D003001:Cloning, Molecular; D056738:Cross Protection; D000939:Epitopes / Q000276:immunology; D005393:Fish Diseases / Q000517:prevention & control; D008969:Molecular Sequence Data; D018272:Porins / Q000235:genetics / Q000276:immunology*; D011817:Rabbits; D011991:Receptors, Virus / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D014733:Vibrio / Q000235:genetics*; D014735:Vibrio Infections / Q000517:prevention & control; D015027:Zebrafish
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D000939:Epitopes; D018272:Porins; D011991:Receptors, Virus; D011994:Recombinant Proteins; C016532:maltoporins
keywords: LamB (maltoporin); Outer membrane protein; Versatile vaccine; Vibrio