title: Measuring the role for Met endosomal signaling in tumorigenesis.
abstract: Met is a receptor tyrosine kinase, often overexpressed or mutated in human cancer. Upon activation by its ligand, the hepatocyte growth factor, Met controls several cell functions such as proliferation, migration, and survival through the activation of multiple pathways. Upon ligand binding, Met rapidly internalizes and continues to signal from endosomal compartments prior to its degradation. Importantly, this "endosomal signaling" has recently been shown to be involved in tumorigenesis and experimental metastasis. Consequently, interfering with Met endosomal signaling may provide a novel therapeutic approach in cancer treatment. However, there is a need for additional studies in various experimental models to confirm this and find the most specific ways of achieving it. Thus, outlined in this review are the techniques and tools we have been using to study Met endocytosis and Met endosomal signaling.
authors.affiliation: Centre for Tumour Biology, Barts Cancer Institute - A Cancer Research UK Centre of Excellence, Queen Mary University of London, John Vane Science Centre, London, United Kingdom.
authors.forename: Rachel
authors.initials: R
authors.lastname: Barrow
authors.affiliation: Centre for Tumour Biology, Barts Cancer Institute - A Cancer Research UK Centre of Excellence, Queen Mary University of London, John Vane Science Centre, London, United Kingdom; UNITE 830 INSERM, Institut Curie Centre de Recherche, Paris Cedex 05, France.
authors.forename: Carine
authors.initials: C
authors.lastname: Joffre
authors.affiliation: Centre for Tumour Biology, Barts Cancer Institute - A Cancer Research UK Centre of Excellence, Queen Mary University of London, John Vane Science Centre, London, United Kingdom.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Ménard
authors.affiliation: Centre for Tumour Biology, Barts Cancer Institute - A Cancer Research UK Centre of Excellence, Queen Mary University of London, John Vane Science Centre, London, United Kingdom. Electronic address: s.kermorgant@qmul.ac.uk.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Kermorgant
mesh_terms: D000818:Animals; D001665:Binding Sites; D019921:Biotinylation; D063646:Carcinogenesis / Q000378:metabolism*; D045744:Cell Line, Tumor; D011992:Endosomes / Q000378:metabolism*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted; D051379:Mice; D008819:Mice, Nude; D041681:NIH 3T3 Cells; D009368:Neoplasm Transplantation; D021381:Protein Transport; D059748:Proteolysis; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism*; D015398:Signal Transduction; D013194:Staining and Labeling
chemical_list: C066855:HGF protein, human; D017228:Hepatocyte Growth Factor; C491743:MET protein, human; D019859:Proto-Oncogene Proteins c-met
keywords: Biotinylation trafficking assays; Endosomal signaling; Endosome; HGF; Met; Trafficking; Tumorigenesis