title: Rapid detection of neutralizing antibodies against bovine viral diarrhoea virus using quantitative high-content screening.
abstract: Bovine viral diarrhoea virus (BVDV) is an important cause of morbidity, mortality and economic losses in cattle worldwide. Humoral immunity to BVDV plays a major role in the protection against infection and disease. In vitro serum neutralization tests can quantify humoral responses, but standard protocols are time-consuming and labour-intensive. The objective of this study was to develop a highly sensitive assay based on high-content cell-by-cell screening that is faster and less subjective than the conventional protocols. It can detect a neutralizing antibody response within the first week after infection of an animal, takes less than 24h to complete and excludes operator bias by automated data acquisition and analysis.
authors.affiliation: Faculty of Veterinary Medicine, University of Calgary, 3280 Hospital Drive NW, Calgary, AB T2N 4N1, Canada. Electronic address: meschbau@ucalgary.ca.
authors.forename: Michael
authors.initials: M
authors.lastname: Eschbaumer
authors.affiliation: Faculty of Veterinary Medicine, University of Calgary, 3280 Hospital Drive NW, Calgary, AB T2N 4N1, Canada.
authors.forename: Sampson
authors.initials: S
authors.lastname: Law
authors.affiliation: Faculty of Veterinary Medicine, University of Calgary, 3280 Hospital Drive NW, Calgary, AB T2N 4N1, Canada.
authors.forename: Cristina
authors.initials: C
authors.lastname: Solis
authors.affiliation: Faculty of Veterinary Medicine, University of Calgary, 3280 Hospital Drive NW, Calgary, AB T2N 4N1, Canada.
authors.forename: Adam
authors.initials: A
authors.lastname: Chernick
authors.affiliation: Faculty of Veterinary Medicine, University of Calgary, 3280 Hospital Drive NW, Calgary, AB T2N 4N1, Canada.
authors.forename: Frank
authors.initials: F
authors.lastname: van der Meer
authors.affiliation: Faculty of Veterinary Medicine, University of Calgary, 3280 Hospital Drive NW, Calgary, AB T2N 4N1, Canada.
authors.forename: Markus
authors.initials: M
authors.lastname: Czub
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology* / Q000821:virology; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D007118:Immunoassay / Q000379:methods*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral
keywords: BVDV; Dose–response curves; High-throughput; IN Cell Analyzer; Neutralization assay; Nonlinear regression