title: Arctiin induces an UVB protective effect in human dermal fibroblast cells through microRNA expression changes.
abstract: Ultraviolet (UV) radiation induces severe alterations in the molecular and cellular components of normal human dermal fibroblast (NHDF) cells by disrupting many intracellular transduction cascades. Although UV responses have been well documented at the genome and proteome levels, UV protective effects have not been elucidated at these levels. The aim of the present study was to demonstrate that arctiin, a phytochemical isolated from the plant Arctium lappa, induced a protective effect against UVB radiation by changing microRNA (miRNA) expression profiles. Using flow cytometry, and water-soluble tetrazolium salt (WST-1)-based cell viability, wound healing, and DNA repair assays we showed that pretreatment with arctiin prior to UVB irradiation reduced UVB-induced apoptosis, cell migration defects, and DNA damage in NHDF cells. It was also found that arctiin‑induced UVB protection is associated with altered miRNA expression profiles. Bioinformatic analysis revealed that the deregulated miRNAs were functionally involved in mitogen-activated protein kinase (MAPK) signaling and cancer signaling pathways. The results suggest that arctiin acts as a UVB protective agent by altering specific miRNA expression in NHDF cells.
authors.affiliation: Songpa R&D Center, Coreana Cosmetics Co., Ltd., Cheonan, Chungcheongnam-do 330-833, Republic of Korea.
authors.forename: Ghang Tai
authors.initials: GT
authors.lastname: Lee
authors.affiliation: Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Hwa Jun
authors.initials: HJ
authors.lastname: Cha
authors.affiliation: Songpa R&D Center, Coreana Cosmetics Co., Ltd., Cheonan, Chungcheongnam-do 330-833, Republic of Korea.
authors.forename: Kwang Sik
authors.initials: KS
authors.lastname: Lee
authors.affiliation: Songpa R&D Center, Coreana Cosmetics Co., Ltd., Cheonan, Chungcheongnam-do 330-833, Republic of Korea.
authors.forename: Kun Kook
authors.initials: KK
authors.lastname: Lee
authors.affiliation: College of Pharmacy and Medical Research Center, Chungbuk National University, Cheongju, Chungcheongbuk-do 361-763, Republic of Korea.
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
authors.affiliation: Department of Dermatology, Konkuk University School of Medicine, Seoul 143-701, Republic of Korea.
authors.forename: Kyu Joong
authors.initials: KJ
authors.lastname: Ahn
authors.affiliation: Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: In-Sook
authors.initials: IS
authors.lastname: An
authors.affiliation: Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Sungkwan
authors.initials: S
authors.lastname: An
authors.affiliation: Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Seunghee
authors.initials: S
authors.lastname: Bae
mesh_terms: D031170:Arctium / Q000737:chemistry; D005347:Fibroblasts / Q000187:drug effects* / Q000528:radiation effects; D005663:Furans / Q000008:administration & dosage* / Q000737:chemistry; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D005960:Glucosides / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry; D011837:Radiation-Protective Agents / Q000008:administration & dosage* / Q000737:chemistry; D015398:Signal Transduction / Q000187:drug effects* / Q000528:radiation effects; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D005663:Furans; D005960:Glucosides; D035683:MicroRNAs; D010936:Plant Extracts; D011837:Radiation-Protective Agents; C077992:arctiin