title: Secretome analysis of human oligodendrocytes derived from neural stem cells.
abstract: In this study, we investigated the secretome of human oligodendrocytes (F3.Olig2 cells) generated from human neural stem cells by transduction with the gene encoding the Olig2 transcription factor. Using mRNA sequencing and protein cytokine arrays, we identified a number of biologically important secretory proteins whose expression has not been previously reported in oligodendrocytes. We found that F3.Olig2 cells secrete IL-6, PDGF-AA, GRO, GM-CSF, and M-CSF, and showed prominent expression of their corresponding receptors. Co-expression of ligands and receptors suggests that autocrine signaling loops may play important roles in both differentiation and maintenance of oligodendrocytes. We also found that F3.Olig2 cells secrete matrix metalloproteinases and matrix metalloproteinase-associated proteins associated with functional competence of oligodendrocytes. The results of our secretome analysis provide insights into the functional and molecular details of human oligodendrocytes. To the best of our knowledge, this is the first systematic analysis of the secretome of oligodendrocytes.
authors.affiliation: Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon, Korea ; Asan Center for Cancer Genome Discovery, Asan Institute for Life Science, Ulsan University College of Medicine, Asan Medical Center, Seoul, Korea ; BRC Genome Research Center, Bio Research Complex, Incheon, Korea.
authors.forename: Woo Kyung
authors.initials: WK
authors.lastname: Kim
authors.affiliation: Asan Center for Cancer Genome Discovery, Asan Institute for Life Science, Ulsan University College of Medicine, Asan Medical Center, Seoul, Korea.
authors.forename: Deokhoon
authors.initials: D
authors.lastname: Kim
authors.affiliation: Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon, Korea.
authors.forename: Jun
authors.initials: J
authors.lastname: Cui
authors.affiliation: Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon, Korea.
authors.forename: Ho Hee
authors.initials: HH
authors.lastname: Jang
authors.affiliation: Medical Research Institute, Chung-Ang University College of Medicine, Seoul, Korea.
authors.forename: Kwang Sei
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Medical Research Institute, Chung-Ang University College of Medicine, Seoul, Korea.
authors.forename: Hong Jun
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Medical Research Institute, Chung-Ang University College of Medicine, Seoul, Korea ; Division of Neurology, Department of Medicine, UBC Hospital, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Seung U
authors.initials: SU
authors.lastname: Kim
authors.affiliation: Asan Center for Cancer Genome Discovery, Asan Institute for Life Science, Ulsan University College of Medicine, Asan Medical Center, Seoul, Korea ; Department of Oncology, Ulsan University College of Medicine, Seoul, Korea.
authors.forename: Sung-Min
authors.initials: SM
authors.lastname: Ahn
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers; D002454:Cell Differentiation / Q000235:genetics; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D000076104:Oligodendrocyte Transcription Factor 2; D009836:Oligodendroglia / Q000166:cytology* / Q000378:metabolism*; D009928:Organ Specificity / Q000235:genetics; D020543:Proteome; D040901:Proteomics / Q000379:methods; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014161:Transduction, Genetic
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D015415:Biomarkers; D016207:Cytokines; D009419:Nerve Tissue Proteins; D000076104:Oligodendrocyte Transcription Factor 2; D020543:Proteome; D011956:Receptors, Cell Surface; C405708:OLIG2 protein, human