title: Ovarian stimulation by exogenous gonadotropin decreases the implantation rate and expression of mouse blastocysts integrins.
abstract: BACKGROUND
  Integrins are heterodimeric glycoprotein receptors that regulate the interaction of cells with extracellular matrix and may have a critical role in implantation. The aim of this study was to investigate the effect of ovulation induction on the expression of α4, αv, β1, and β3 integrins in mouse blastocyst at the time of implantation.
  
  
  METHODS
  The ovarian stimulated and non-stimulated pregnant mice were sacrificed on the morning of 5th day of pregnancy. The blastocysts were collected, and the expression of αv, α4, β1, and β3 integrins was examined using real-time RT-PCR and immunocytochemical techniques, then their ovarian hormones were analyzed at the same time. The implantation sites in uterine horns of other pregnant mice in both groups were determined under a stereomicroscope on the 7th day of pregnancy.
  
  
  RESULTS
  The results showed that the expression of αv, β1, and β3 integrins in both mRNA and protein levels was significantly lower in the ovarian stimulated group than the control group, and the maximum ratio of expression was belonged to β1 molecule (P>0.05).
  
  
  CONCLUSIONS
  The implantation rate in superovulated mice was significantly lower than control mice. It was suggested that ovulation induction decreased the expression of αv, β1, and β3 integrins of mouse blastocysts.
authors.affiliation: Dept. of Anatomy, Tarbiat Modares University, Tehran, Iran. salehnim@modares.ac.ir.
authors.forename: Mehri
authors.initials: M
authors.lastname: Fayazi
authors.forename: Mandana
authors.initials: M
authors.lastname: Beigi Boroujeni
authors.forename: Mojdeh
authors.initials: M
authors.lastname: Salehnia
authors.forename: Behzad
authors.initials: B
authors.lastname: Khansarinejad
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology; D010064:Embryo Implantation / Q000187:drug effects* / Q000502:physiology; D004958:Estradiol / Q000097:blood; D005260:Female; D006062:Gonadotropins / Q000494:pharmacology*; D039441:Integrin alpha4 / Q000096:biosynthesis / Q000235:genetics; D039564:Integrin alphaV / Q000096:biosynthesis / Q000235:genetics; D019012:Integrin beta1 / Q000096:biosynthesis / Q000235:genetics; D039661:Integrin beta3 / Q000096:biosynthesis / Q000235:genetics; D016023:Integrins / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D010053:Ovary / Q000187:drug effects*; D010062:Ovulation Induction; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D006062:Gonadotropins; D039564:Integrin alphaV; D019012:Integrin beta1; D039661:Integrin beta3; D016023:Integrins; D012333:RNA, Messenger; D039441:Integrin alpha4; D011374:Progesterone; D004958:Estradiol