title: Coordinate bonding strategy for molecularly imprinted hydrogels: toward pH-responsive doxorubicin delivery.
abstract: Molecularly imprinted hydrogel (MIH) as drug delivery system has been studied. It still remains a challenge to construct the stimuli-responsive MIH. Here, we report a coordinate bond strategy for imprinting doxorubicin (Dox) in hydrogel capable of pH-responsive and sustained drug delivery. The imprinting condition such as template-monomer interactions induced by metal ion was carefully investigated by spectroscopic methods. The obtained Dox-MIH was evaluated by absorption and in vitro release experiments. It has been demonstrated that the cupric ion mediated interaction between Dox and 4-vinyl pyridine via coordination and the optimal coordinate ratio of Dox/Cu(2+) was 2:1. The rebinding amount of MIH to Dox was 2.7-fold that of nonimprinted hydrogel and the Dox-loaded MIH showed a pH-responsive release property. Not more than 10% of loaded drug was released from Dox-MIH at pH 7.2 during a time course of 7 days. However, near to 60% of loaded drug was sustainedly released at pH 5.0 during the same period. These results indicated that Dox-MIH with pH-responsive behavior possessed great promising as sustained-release delivery system of anticancer drug.
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing, 211166, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.forename: Liying
authors.initials: L
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Shuhu
authors.initials: S
authors.lastname: Du
mesh_terms: D000042:Absorption; D000178:Acrylamides / Q000737:chemistry; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D002626:Chemistry, Pharmaceutical; D003432:Cross-Linking Reagents; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D016503:Drug Delivery Systems; D020100:Hydrogels / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000737:chemistry; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics
chemical_list: D000178:Acrylamides; D000903:Antibiotics, Antineoplastic; D003432:Cross-Linking Reagents; D020100:Hydrogels; D008689:Methacrylates; C491267:N-methylacrylamide; C005044:hydroxyethyl methacrylate; D004317:Doxorubicin
keywords: cancer chemotherapy; complexation; controlled release; coordinate bonding; doxorubicin; drug delivery systems; hydrogel; molecular imprinting; pH response