title: H2O2-dependent substrate oxidation by an engineered diiron site in a bacterial hemerythrin.
abstract: The O2-binding carboxylate-bridged diiron site in DcrH-Hr was engineered in an effort to perform the H2O2-dependent oxidation of external substrates. A His residue was introduced near the diiron site in place of a conserved residue, Ile119. The I119H variant promotes the oxidation of guaiacol and 1,4-cyclohexadiene upon addition of H2O2.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan. onoda@chem.eng.osaka-u.ac.jp thayashi@chem.eng.osaka-u.ac.jp.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Okamoto
authors.forename: Akira
authors.initials: A
authors.lastname: Onoda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugimoto
authors.forename: Yu
authors.initials: Y
authors.lastname: Takano
authors.forename: Shun
authors.initials: S
authors.lastname: Hirota
authors.forename: Donald M
authors.initials: DM
authors.lastname: Kurtz
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Shiro
authors.forename: Takashi
authors.initials: T
authors.lastname: Hayashi
mesh_terms: D001419:Bacteria / Q000737:chemistry*; D006422:Hemerythrin / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007501:Iron / Q000737:chemistry*; D010084:Oxidation-Reduction
chemical_list: D006422:Hemerythrin; D006861:Hydrogen Peroxide; D007501:Iron