title: [Determination of environmental estrogens in cow feed and soil by high performance liquid chromatography-tandem mass spectrometry].
abstract: An analytical method was developed for the determination of the residues of five environmental estrogens, including estriol, estradiol, estrone, bisphenol A and diethylstilbestrol, in the cow feed and soil by solid phase extraction (SPE) and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The important parameters which affect the determination efficiency such as the mobile phase, the condition of mass spectrometry and solid phase extraction column were optimized. The optimal determination conditions were as follows: the sample was extracted with acetonitrile at first, then cleaned-up with an NH2-SPE column, and an Acquity UPLC HSS T3 column was selected to separate the analytes. Acetonitrile-methanol (4: 1, v/v) and 0.01% ammonia aqueous solution were used as the mobile phase by gradient elution in the negative mode. The limits of detection (LOD, S/N = 3) were 0.06 - 0.22 microg/kg. The overall recoveries varied from 81.70% to 102.20%, and the relative standard deviations (RSDs) were all less than 10.00%. This method is simple, sensitive and accurate, and suitable for the determination of environmental estrogens in cow feed and soil.
authors.affiliation: College of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
authors.forename: Guangqing
authors.initials: G
authors.lastname: Mu
authors.affiliation: College of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Chen
authors.affiliation: Beijing Sanyuan Foods Co., Ltd., Beijing 100076, China.
authors.forename: Tiemin
authors.initials: T
authors.lastname: Jiang
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D001559:Benzhydryl Compounds; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004054:Diethylstilbestrol; D004958:Estradiol; D004964:Estriol; D004967:Estrogens / Q000032:analysis*; D004970:Estrone; D010636:Phenols; D012987:Soil / Q000737:chemistry*; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004054:Diethylstilbestrol; D004958:Estradiol; D004964:Estriol; D004967:Estrogens; D004970:Estrone; D010636:Phenols; D012987:Soil; C006780:bisphenol A; D000098826:Bisphenol A Compounds