title: Synthesis and application of a targeting diagnosis system via quantum dots coated by amphiphilic polymer for the detection of liver cancer cells.
abstract: Water-soluble quantum dots (QDs) for liver cancer diagnosis were prepared using QDs with oleylamine ligand coated with poly(aspartate)-graft-poly(ethylene glycol)-dodecylamine (PASP-Na-g-PEG-DDA). Dynamic light scattering and transmission electron microscopy imaging showed that the novel QDs have an ellipsoidal morphology with a size of ~ 45 nm which could be used for biomedical application. Furthermore, the PASP-Na-g-PEG-DDA was then modified with anti-(vascular endothelial growth factor) (VEGF antibody), and a 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan (MTT) assay showed that the novel anti-VEGF-targeting QDs in vitro had low toxicity. Confocal laser scanning microscopy observations revealed an intracellular (HepG2) distribution of the novel anti-VEGF-targeting QDs and the targeting efficiency of anti-VEGF. These novel QDs could be used as a probe for liver cancer cell imaging because of anti-VEGF targeting.
authors.affiliation: Alan G. MacDiarmid Institute of Jilin University, Changchun, 130012, China.
authors.forename: Xiaoran
authors.initials: X
authors.lastname: Sun
authors.forename: Yapeng
authors.initials: Y
authors.lastname: Li
authors.forename: Hailong
authors.initials: H
authors.lastname: Huang
authors.forename: Bohan
authors.initials: B
authors.lastname: Yang
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Wang
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis*; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D045663:Quantum Dots; D013329:Structure-Activity Relationship; D013501:Surface-Active Agents / Q000737:chemistry / Q000494:pharmacology
chemical_list: D020099:Coated Materials, Biocompatible; D011108:Polymers; D013501:Surface-Active Agents
keywords: PASP-Na-g-PEG-DDA; cytotoxicity; intracellular fluorescence; quantum dots; target