title: [Determination of taxifolin in Polygonum orientale of different storage period].
abstract: OBJECTIVE
  To find out the correlation between the content of taxifolin in Polygonum orientale and the storage time.
  
  
  METHODS
  HPLC was used to determine taxifolin. The chromatographic condition was as following: Diamonsil C18 column (4.6 mm x 200 mm, 5 microm), mobile phase acetonitrile -0.1% phosphoric acid (gradient elution), the detection wavelength 290 nm and flow rate 1.0 mL x min(-1), the column temperature 30 degrees C.
  
  
  RESULTS
  The injection volume of taxifolin was in good linearity within 0.07 and 0.35 microg, the average recovery was 99.7% with RSD 0.2%. Taxifolin content was 0.84, 1.36, 1.75, 1.99 mg x g(-1) corresponding to storage time of 10, 7, 6, 5 years, respectively.
  
  
  CONCLUSIONS
  The content of taxifolin decreased with the storage time. When the storage period is more than six years, the content is lower than that required by Chinese Pharmacopoeia (2010 version). This method has a good repeatability and accuracy, it provides a scientific reference for clinical use and quality evaluation of P. orientale.
authors.affiliation: College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China. 393193876@qq.com
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Tan
authors.affiliation: College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Tong
authors.affiliation: College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.
authors.forename: Yu-Yao
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.
authors.forename: Yang-Yang
authors.initials: YY
authors.lastname: Xu
authors.affiliation: College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Zhai
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D004356:Drug Storage / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D028502:Polygonum / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D004365:Drugs, Chinese Herbal; D011794:Quercetin; C003377:taxifolin