title: Inhibition of 2,4-dinitrofluorobenzene-induced contact hypersensitivity reaction by antidepressant drugs.
abstract: BACKGROUND
  Contact hypersensitivity (CHS) induced by a topical application of hapten - 2,4-dinitrofluorobenzene (DNFB), is a T cytotoxic (Tc)1-cell-mediated antigen-specific type of skin inflammation. Recently, it has been shown that antidepressant drugs inhibit the T helper (Th)1-mediated CHS reaction induced by picryl chloride. The aim of present study was to establish the effect of two-week desipramine or fluoxetine administration on the CHS reaction induced by DNFB.
  
  
  METHODS
  Balb/c (H-2(d)) male mice were divided into six groups: 1) vehicle-treated negative control group; 2) desipramine-treated negative control group; 3) fluoxetine-treated negative control group; 4) vehicle-treated DNFB group (positive control group); 5) desipramine-treated DNFB group; 6) fluoxetine-treated DNFB group. T lymphocytes proliferation was determined by incorporation of [(3)H]-thymidine to DNA of concanavalin A stimulated cells. ELISA test was used for estimation of cytokines production.
  
  
  RESULTS
  The antidepressants significantly suppressed the CHS reaction mediated by Tc1 cells: desipramine by 55% and fluoxetine by 54% compared to the positive control. Moreover, the antidepressants decreased the proliferative activity of splenocytes and the ability of splenocytes to produce interleukin (IL)-6 and interferon (IFN)-γ and increased IL-10 production by the lymph node (LN) cells of DNFB-treated mice.
  
  
  CONCLUSIONS
  The results of the present study show that the Tc1-dependent reactivity to DNFB is significantly suppressed by antidepressant drugs, which suggests their inhibitory effect on Tc1 mediated immunity.
authors.affiliation: Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Science, Smętna 12, PL 31-343 Kraków, Poland. kubera@if-pan.krakow.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Curzytek
authors.forename: Marta
authors.initials: M
authors.lastname: Kubera
authors.forename: Monika
authors.initials: M
authors.lastname: Majewska-Szczepanik
authors.forename: Marian
authors.initials: M
authors.lastname: Szczepanik
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Marcińska
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Ptak
authors.forename: Weronika
authors.initials: W
authors.lastname: Duda
authors.forename: Monika
authors.initials: M
authors.lastname: Leśkiewicz
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Basta-Kaim
authors.forename: Bogusława
authors.initials: B
authors.lastname: Budziszewska
authors.forename: Władysław
authors.initials: W
authors.lastname: Lasoń
authors.forename: Michael
authors.initials: M
authors.lastname: Maes
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D003877:Dermatitis, Contact / Q000209:etiology / Q000276:immunology / Q000517:prevention & control*; D003891:Desipramine / Q000494:pharmacology*; D004139:Dinitrofluorobenzene; D004195:Disease Models, Animal; D005473:Fluoxetine / Q000494:pharmacology*; D007111:Immunity, Cellular / Q000187:drug effects*; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008198:Lymph Nodes / Q000187:drug effects / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects* / Q000276:immunology; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology
chemical_list: D000928:Antidepressive Agents; C556062:IL10 protein, mouse; D015850:Interleukin-6; C577686:interleukin-6, mouse; D005473:Fluoxetine; D016753:Interleukin-10; D007371:Interferon-gamma; D004139:Dinitrofluorobenzene; D003891:Desipramine