title: A T7 exonuclease-assisted cyclic enzymatic amplification method coupled with rolling circle amplification: a dual-amplification strategy for sensitive and selective microRNA detection.
abstract: A T7 exonuclease-assisted cyclic enzymatic amplification method (CEAM) was combined with rolling circle amplification (RCA) to develop a RCA-CEAM dual amplification method for ultrasensitive detection of microRNA with excellent selectivity.
authors.affiliation: State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, P. R. China. zhuzhi@xmu.edu.cn cyyang@xmu.edu.cn.
authors.forename: Liang
authors.initials: L
authors.lastname: Cui
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhu
authors.forename: Ninghang
authors.initials: N
authors.lastname: Lin
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Guan
authors.forename: Chaoyong James
authors.initials: CJ
authors.lastname: Yang
mesh_terms: D017123:Bacteriophage T7 / Q000201:enzymology*; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D009369:Neoplasms / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*
chemical_list: D035683:MicroRNAs; D005090:Exodeoxyribonucleases; C046264:gene b exonuclease