title: Structure and properties of thermoplastic polyurethanes based on poly(dimethylsiloxane): assessment of biocompatibility.
abstract: Properties and biocompatibility of a series of thermoplastic poly(urethane-siloxane)s (TPUSs) based on α,ω-dihydroxy ethoxy propyl poly(dimethylsiloxane) (PDMS) for potential biomedical application were studied. Thin films of TPUSs with a different PDMS soft segment content were characterized by (1) H NMR, quantitative (13) C NMR, Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), differential scanning calorimetry (DSC), dynamic mechanical analysis (DMA), contact angle, and water absorption measurements. Different techniques (FTIR, AFM, and DMA) showed that decrease of PDMS content promotes microphase separation in TPUSs. Samples with a higher PDMS content have more hydrophobic surface and better waterproof performances, but lower degree of crystallinity. Biocompatibility of TPUSs was examined after attachment of endothelial cells to the untreated copolymer surface or surface pretreated with multicomponent protein mixture, and by using competitive protein adsorption assay. TPUSs did not exhibit any cytotoxicity toward endothelial cells, as measured by lactate dehydrogenase and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide assays. The untreated and proteins preadsorbed TPUS samples favored endothelial cells adhesion and growth, indicating good biocompatibility. All TPUSs adsorbed more albumin than fibrinogen in competitive protein adsorption experiment, which is feature regarded as beneficial for biocompatibility. The results indicate that TPUSs have good surface, thermo-mechanical, and biocompatible properties, which can be tailored for biomedical application requirements by adequate selection of the soft/hard segments ratio of the copolymers.
authors.affiliation: Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoševa 12, Belgrade, 11000, Serbia.
authors.forename: Marija V
authors.initials: MV
authors.lastname: Pergal
authors.forename: Jelena
authors.initials: J
authors.lastname: Nestorov
authors.forename: Gordana
authors.initials: G
authors.lastname: Tovilović
authors.forename: Sanja
authors.initials: S
authors.lastname: Ostojić
authors.forename: Dejan
authors.initials: D
authors.lastname: Gođevac
authors.forename: Dana
authors.initials: D
authors.lastname: Vasiljević-Radović
authors.forename: Jasna
authors.initials: J
authors.lastname: Djonlagić
mesh_terms: D000327:Adsorption; D002448:Cell Adhesion; D002460:Cell Line; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D042783:Endothelial Cells / Q000166:cytology / Q000201:enzymology*; D005340:Fibrinogen / Q000737:chemistry; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis*; D008422:Materials Testing; D008567:Membranes, Artificial; D011140:Polyurethanes / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D004129:Dimethylpolysiloxanes; D008567:Membranes, Artificial; D011140:Polyurethanes; D012710:Serum Albumin, Bovine; C013830:baysilon; D005340:Fibrinogen; D007770:L-Lactate Dehydrogenase
keywords: biocompatibility; cell adhesion; competitive protein adsorption; poly(urethane-siloxane) films