title: Astragalosides IV inhibits high glucose-induced cell apoptosis through HGF activation in cultured human tubular epithelial cells.
abstract: Astragaloside IV (ASI) in Radix Astragali is believed to be the active component. The study aims to investigate whether ASI inhibits tubular epithelial cells apoptosis induced by high glucose and its mechanisms. Tubular epithelial cells in this paper were isolated from human kidney. The cells apoptosis was detected by TUNEL and caspase 3 assay. The protein levels of HGF and TGF-β1 were measured by ELISA. The phospho-p38 production, ERK and JNK were determined by Western blot. ASI could inhibit cells apoptosis induced by high glucose (25 mmol/L) in dose-dependent and time-dependent manners. ASI also inhibited high glucose-induced expression of TGF-β1 and activation of p38 MAPK pathway at the protein level. Furthermore, ASI increased HGF production in human tubular epithelial cells. The ASI inhibition of tubular epithelial cells apoptosis and reduction of TGF-β1 expression induced by high glucose may represent a new treatment for diabetic kidney injury. The mechanism underlying this inhibitory effect may be related to the inhibition of p38 MAPK signaling pathway activation and HGF overproduction.
authors.affiliation: Department of Nephrology, Molecular Cell Lab for Kidney Disease, Ren Ji Hospital, School of Medicine, Shanghai JiaoTong University , Shanghai , PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.forename: Xinghua
authors.initials: X
authors.lastname: Shao
authors.forename: Weijia
authors.initials: W
authors.lastname: Xu
authors.forename: Chaojun
authors.initials: C
authors.lastname: Qi
authors.forename: Leyi
authors.initials: L
authors.lastname: Gu
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Ni
authors.forename: Shan
authors.initials: S
authors.lastname: Mou
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D004847:Epithelial Cells; D005947:Glucose / Q000008:administration & dosage; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006801:Humans; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000473:pathology*; D012503:Saponins / Q000008:administration & dosage / Q000494:pharmacology*; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014315:Triterpenes / Q000008:administration & dosage / Q000494:pharmacology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins; D016212:Transforming Growth Factor beta; D014315:Triterpenes; C052064:astragaloside A; D017228:Hepatocyte Growth Factor; D048051:p38 Mitogen-Activated Protein Kinases; D005947:Glucose