title: Expansion of CD4(+) HLA-G(+) T Cell in human pregnancy is impaired in pre-eclampsia.
abstract: OBJECTIVE
  The role of CD4(+) HLA-G(+) T cells in healthy pregnancy and pre-eclampsia is unclear.
  
  
  METHODS
  CD4(+) HLA-G(+) T cells were analysed from peripheral blood and decidual samples from healthy pregnant and pre-eclamptic women. In vitro T-cell induction, trogocytosis and suppression assays were performed.
  
  
  RESULTS
  In peripheral blood, CD4(+) HLA-G(+) T cells were significantly higher in pregnant women (mean ± S.E.M.: 7.98 ± 1.10%), compared with non-pregnant controls (mean ± S.E.M.: 1.78 ± 0.30%) and pre-eclamptic women (mean ± S.E.M.: 3.69 ± 0.51%). The presence of CD4(+) HLA-G(+) T cells is even more prominent in the decidua, suggestive of local induction and accumulation. Decidual CD14(+) DC-SIGN(+) antigen-presenting cells (APCs) enhance the HLA-G expression of cocultured CD4(+) naïve T cells in vitro. IL-10 augments expression of HLA-G, ILT4 and DC-SIGN in monocyte-derived DCs (MoDCs), endowing them with a phenotype analogous to decidual CD14(+) DC-SIGN(+) APCs of healthy pregnancy. Furthermore, naïve T cells acquire HLA-G from these IL-10-treated MoDCs via the process of trogocytosis.
  
  
  CONCLUSIONS
  Our data indicate that in addition to Foxp3(+) Treg cells, CD4(+) T cells acquire HLA-G from decidual DCs and may play an important role in immune tolerance induction in pregnancy, a process which is impaired in pre-eclampsia.
authors.affiliation: Sydney Medical School Nepean, The University of Sydney, Kingswood, NSW, Australia; Department of Allergy and Immunology, The Children's Hospital at Westmead, Westmead, NSW, Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Hsu
authors.forename: Brigitte
authors.initials: B
authors.lastname: Santner-Nanan
authors.forename: Steven
authors.initials: S
authors.lastname: Joung
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Peek
authors.forename: Ralph
authors.initials: R
authors.lastname: Nanan
mesh_terms: D000328:Adult; D001773:Blood Cells / Q000276:immunology*; D015704:CD4 Antigens / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018920:Coculture Techniques; D003656:Decidua / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D004705:Endocytosis / Q000276:immunology; D005260:Female; D059951:HLA-G Antigens / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007108:Immune Tolerance; D016753:Interleukin-10 / Q000276:immunology; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D011225:Pre-Eclampsia / Q000276:immunology*; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D015854:Up-Regulation; D055815:Young Adult; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015704:CD4 Antigens; D015815:Cell Adhesion Molecules; D059951:HLA-G Antigens; D016753:Interleukin-10; D037181:Lectins, C-Type; D018950:Lipopolysaccharide Receptors; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C491341:LILRB2 protein, human
keywords: CD4+HLA-G+ T cells; human pregnancy; immune tolerance