title: [Studies of interaction between surface of pirolytic carbon and blood cells and proteins].
abstract: BACKGROUND
  Preventing blood clots formation on the biomaterials surface and finding the causes of their appearance are the leading research subjects while working out biomaterials for grafts into the circulation system. Interaction between the biological environment and the carbon implant is closely connected with the properties of its surface. Implants whith surfaces covered with pyrolytic carbon is characterised by athrombogenity have particular significance in medical applications.
  
  
  OBJECTIVE
  Assessment of the influence of a pyrolytic carbon surface on the activation of coagulation system.
  
  
  METHODS
  Full human blood was subjected to temporal contact in vitro with the surface of low-temperature isotropic carbon (LTI). The haemostatic action of pyrolytic carbon was determined through marking the chosen parameters of the coagulation system and the recalcification time on the material surface. The evaluation of the topography of the material was performed in the scanning microscopy.
  
  
  RESULTS
  Changes in the values APTT, PT, TT, fibrinogen and activity of factors F XII, F IX, F VIII as well as AT III, protein C and plasminogen were not observed in the studies of the plasmatic coagulation system. The blood coagulation time on the material surface was elongated in comparison with the surface of glass and polystyrene surface.
  
  
  CONCLUSIONS
  Direct contact of blood with the surface of carbon LTI elongates clot formation, while not changing the parameters of the plasmatic coagulation system in temporal contact.
authors.affiliation: Zakład Chirurgii Eksperymentalnej i Badania Biomateriałów, Uniwersytet Medyczny im. Piastów Slaskich we Wrocławiu, Wrocław, Polska. maria.szymonowicz@umed.wroc.pl
authors.forename: Maria
authors.initials: M
authors.lastname: Szymonowicz
authors.affiliation: Zakład Chirurgii Eksperymentalnej i Badania Biomateriałów, Uniwersytet Medyczny im. Piastów Slaskich we Wrocławiu, Wrocław, Polska.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Rybak
authors.affiliation: Zakład Chirurgii Eksperymentalnej i Badania Biomateriałów, Uniwersytet Medyczny im. Piastów Slaskich we Wrocławiu, Wrocław, Polska.
authors.forename: Danuta
authors.initials: D
authors.lastname: Paluch
authors.affiliation: Pracownia Mikroskopii Elektronowej, Uniwersytet Przyrodniczy we Wrocławiu, Wrocław, Polska.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Marycz
authors.affiliation: Pracownia Mikroskopii Elektronowej, Uniwersytet Przyrodniczy we Wrocławiu, Wrocław, Polska.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Kaliński
authors.affiliation: Katedra Biomateriałów, Akademia Górniczo-Hutnicza w Krakowie, Kraków, Polska.
authors.forename: Stanisław
authors.initials: S
authors.lastname: Błazewicz
mesh_terms: D001672:Biocompatible Materials / Q000494:pharmacology*; D001773:Blood Cells / Q000166:cytology / Q000187:drug effects*; D001777:Blood Coagulation / Q000187:drug effects*; D002244:Carbon / Q000494:pharmacology*; D006490:Hemostatics / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D006490:Hemostatics; C009139:pyrolytic carbon; D002244:Carbon