title: Regulatory effects of introduction of an exogenous FGF2 gene on other growth factor genes in a healing tendon.
abstract: In this study of a tendon injury model, we investigated how injection of a vector incorporating one growth factor gene changes expression levels of multiple growth factor genes in the healing process. The flexor tendon of chicken toes was completely cut and repaired surgically. The tendons in the experimental arm were injected with an adeno-associated virus-2 vector incorporating basic fibroblast growth-factor gene, whereas the tendons in the control arm were not injected or injected with sham vectors. Using real-time polymerase chain reaction, we found that, within the tendon healing period, a set of growth factor genes-transforming growth factor-β1, vascular endothelial growth factor, and connective tissue growth factor-were significantly up-regulated. Expression of the platelet-derived growth factor-B gene was not changed, and the insulin-like growth factor was down-regulated. A tendon marker gene, scleraxis, was significantly up-regulated in the period. Our study revealed an intriguing finding that introduction of one growth factor gene in the healing tendon modulated expression of multiple growth factor genes. We believe this study may have significant implications in determining the approach of gene therapy, and the findings substantiate that gene therapy using a single growth factor could affect multiple growth factors.
authors.affiliation: Department of Hand Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China; Medical Research Center for Tissue Repair and Reconstruction of Jiangsu, Nantong, Jiangsu, China; Department of Plastic Surgery, Rhode Island Hospital, Alpert Medical School of Brown University, Providence, Rhode Island.
authors.forename: Jin Bo
authors.initials: JB
authors.lastname: Tang
authors.forename: Chuan Hao
authors.initials: CH
authors.lastname: Chen
authors.forename: You Lang
authors.initials: YL
authors.lastname: Zhou
authors.forename: Clarie
authors.initials: C
authors.lastname: McKeever
authors.forename: Paul Y
authors.initials: PY
authors.lastname: Liu
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D002645:Chickens; D055513:Connective Tissue Growth Factor / Q000235:genetics / Q000378:metabolism; D000229:Dependovirus / Q000378:metabolism*; D015536:Down-Regulation; D016222:Fibroblast Growth Factor 2 / Q000235:genetics*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism*; D020574:Proto-Oncogene Proteins c-sis / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013708:Tendon Injuries / Q000473:pathology*; D014034:Toes; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism; D014945:Wound Healing / Q000235:genetics
chemical_list: D030161:Avian Proteins; D051792:Basic Helix-Loop-Helix Proteins; D055513:Connective Tissue Growth Factor; D016222:Fibroblast Growth Factor 2; D007334:Insulin-Like Growth Factor I; D036341:Intercellular Signaling Peptides and Proteins; D020574:Proto-Oncogene Proteins c-sis; D016212:Transforming Growth Factor beta; D042461:Vascular Endothelial Growth Factor A; C503821:SCX protein, Gallus gallus