title: Screening and analysis of metabolites in rat urine after oral administration of Apocynum venetum L. extracts using HPLC-TOF-MS.
abstract: HPLC with diode array detection and ESI-TOF-MS was used for the study of the constituents in Apocynum venetum L. extracts and the metabolites in rat urine after oral administration of A. venetum L. extracts. A formula database of the known constituents in A. venetum L. was established, and 21 constituents were rapidly identified by accurately matching their molecular masses with the formulae of the compounds in the database. Furthermore, 34 metabolites were detected and elucidated in the rat urine. The scientific and plausible biotransformation pathways of the flavonoid components in A. venetum L. were also proposed together with the presentation of clues for potential mechanisms of bioactivity. This specific and sensitive HPLC-ESI-TOF-MS method can be used to identify the chemical components in the extracts of A. venetum L. and their metabolites in rat urine. This method can also be used to reveal the possible metabolic mechanisms of action of the extract components in vivo.
authors.affiliation: Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Shanghai, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.forename: Shanshan
authors.initials: S
authors.lastname: Liang
authors.forename: Lei
authors.initials: L
authors.lastname: Lv
authors.forename: Hai
authors.initials: H
authors.lastname: Zhang
authors.forename: Guang
authors.initials: G
authors.lastname: Guo-Tan
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Chai
authors.forename: Guoqing
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000818:Animals; D030010:Apocynum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000032:analysis* / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014556:Urine / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Apocynum venetum L; HPLC-TOF-MS; Identification; Metabolites; Rat urine