title: Genetic variation of Vibrio cholerae during outbreaks, Bangladesh, 2010-2011.
abstract: Cholera remains a major public health problem. To compare the relative contribution of strains from the environment with strains isolated from patients during outbreaks, we performed multilocus variable tandem repeat analyses on samples collected during the 2010 and 2011 outbreak seasons in 2 geographically distinct areas of Bangladesh. A total of 222 environmental and clinical isolates of V. cholerae O1 were systematically collected from Chhatak and Mathbaria. In Chhatak, 75 of 79 isolates were from the same clonal complex, in which extensive differentiation was found in a temporally consistent pattern of successive mutations at single loci. A total of 59 isolates were collected from 6 persons; most isolates from 1 person differed by sequential single-locus mutations. In Mathbaria, 60 of 84 isolates represented 2 separate clonal complexes. The small number of genetic lineages in isolates from patients, compared with those from the environment, is consistent with accelerated transmission of some strains among humans during an outbreak.
authors.forename: Shah M
authors.initials: SM
authors.lastname: Rashed
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Azman
authors.forename: Munirul
authors.initials: M
authors.lastname: Alam
authors.forename: Shan
authors.initials: S
authors.lastname: Li
authors.forename: David A
authors.initials: DA
authors.lastname: Sack
authors.forename: J Glenn
authors.initials: JG
authors.lastname: Morris
authors.forename: Ira
authors.initials: I
authors.lastname: Longini
authors.forename: Abul Kasem
authors.initials: AK
authors.lastname: Siddique
authors.forename: Anwarul
authors.initials: A
authors.lastname: Iqbal
authors.forename: Anwar
authors.initials: A
authors.lastname: Huq
authors.forename: Rita R
authors.initials: RR
authors.lastname: Colwell
authors.forename: R Bradley
authors.initials: RB
authors.lastname: Sack
authors.forename: O Colin
authors.initials: OC
authors.lastname: Stine
mesh_terms: D001459:Bangladesh / Q000453:epidemiology; D002771:Cholera / Q000453:epidemiology* / Q000266:history / Q000382:microbiology*; D004196:Disease Outbreaks; D014644:Genetic Variation; D005838:Genotype; D049674:History, 21st Century; D006801:Humans; D018598:Minisatellite Repeats; D058885:Multilocus Sequence Typing; D012621:Seasons; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics*
keywords: Bangladesh; Vibrio cholerae; bacteria; cholera; infectious disease outbreaks; multilocus sequence analysis; outbreak