title: Mature embryo-derived wheat transformation for tolerance to moisture stress.
abstract: We have developed marker-free transgenic wheat using a transcription factor, AtDREB1A cloned from Arabidopsis. Southern hybridization confirmed a transgenic event with a single copy insertion. PCR analysis of the T1 plants showed four were positive only for AtDREB1A. A T1 plant (HRCB3#17-37) was marker-free and had good expression of drought tolerance in comparison with untransformed plants. The leaf relative water content of this T1 transgenic plant was 12-15% higher than that of the wild type during stress with an 8% higher yield under water deficit conditions compared with wild type plants.
authors.affiliation: Sugarcane Breeding Institute, Coimbatore, 641 007, India, lakshmimbb@gmail.com.
authors.forename: Lakshmi
authors.initials: L
authors.lastname: Kasirajan
authors.forename: Boomiraj
authors.initials: B
authors.lastname: Kovilpillai
authors.forename: Kailash Chander
authors.initials: KC
authors.lastname: Bansal
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics; D055864:Droughts; D018515:Plant Leaves / Q000737:chemistry; D030821:Plants, Genetically Modified / Q000235:genetics / Q000502:physiology*; D013312:Stress, Physiological / Q000235:genetics / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics; D014908:Triticum / Q000235:genetics / Q000502:physiology*; D014867:Water / Q000032:analysis
chemical_list: D029681:Arabidopsis Proteins; C114578:DREB1A protein, Arabidopsis; D014157:Transcription Factors; D014867:Water