title: Sorting of circulating tumor cells (MV3-melanoma) and red blood cells using non-inertial lift.
abstract: We demonstrate the method of non-inertial lift induced cell sorting (NILICS), a continuous, passive, and label-free cell sorting approach in a simple single layer microfluidic device at low Reynolds number flow conditions. In the experiments, we exploit the non-inertial lift effect to sort circulating MV3-melanoma cells from red blood cell suspensions at different hematocrits as high as 9%. We analyze the separation process and the influence of hematocrit and volume flow rates. We achieve sorting efficiencies for MV3-cells up to EMV3 = 100% at Hct = 9% and demonstrate cell viability by recultivation of the sorted cells.
authors.affiliation: EPI, Soft Matter and Biological Physics, University of Augsburg, D-86159 Augsburg, Germany.
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Geislinger
authors.affiliation: EPI, Soft Matter and Biological Physics, University of Augsburg, D-86159 Augsburg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Franke