title: In vitro drug release and ex vivo percutaneous absorption of resveratrol cream using HPLC with zirconized silica stationary phase.
abstract: Since the designs of optimal formulations for resveratrol permeation via the skin are lacking, the aim of this study was to establish the profile of resveratrol permeability into and across human skin. For that, a laboratory-made chromatographic column was used (Zr-PMODS), with its performance being compared to a traditional C18 column. In vitro drug release was conducted with polysulfone membranes, and the flux (JS) was 30.49 μg cm(-2) h(-1)), with a lag time (LT) of 0.04 h, following a pseudo-first-order kinetics. For ex vivo percutaneous absorption using excised female human skin, the kinetic profile was the same, but JS was 0.87 μg cm(-2) h(-1) and LT was 0.97 h. From the initials 49.30 μg applied to the skin, 9.50 μg were quantified in the receptor medium, 20.48 μg was retained at the stratum corneum (do not account as permeated) and 21.41 μg was retained at the viable epidermis+dermis (account as permeated), totalizing 30.90 μg of resveratrol permeated after 24 h of application (62.6%). From these results, one can conclude that a person using the 1-g emulsion dose released by the pump containing 20mg of resveratrol will have, theoretically, 12.53 mg of it liberated into his bloodstream, gradually and continuously for 24 h.
authors.affiliation: Núcleo de Pesquisa e Inovação em Ciências da Saúde (NUPICS), Federal University of Juiz de Fora, 36036-900 Juiz de Fora, MG, Brazil. Electronic address: h.c.polonini@gmail.com.
authors.forename: Hudson Caetano
authors.initials: HC
authors.lastname: Polonini
authors.affiliation: Grupo de Química Analítica e Quimiometria (GQAQ), Federal University of Juiz de Fora, 36036-900 Juiz de Fora, MG, Brazil.
authors.forename: Carina de Almeida
authors.initials: Cde A
authors.lastname: Bastos
authors.affiliation: Grupo de Química Analítica e Quimiometria (GQAQ), Federal University of Juiz de Fora, 36036-900 Juiz de Fora, MG, Brazil. Electronic address: marcone.oliveira@ufjf.edu.br.
authors.forename: Marcone Augusto Leal
authors.initials: MA
authors.lastname: de Oliveira
authors.affiliation: Institute of Chemistry, University of Campinas, PO Box 6154, 13083-970 Campinas, SP, Brazil.
authors.forename: Carla Grazieli Azevedo
authors.initials: CG
authors.lastname: da Silva
authors.affiliation: Institute of Chemistry, University of Campinas, PO Box 6154, 13083-970 Campinas, SP, Brazil.
authors.forename: Carol Hollingworth
authors.initials: CH
authors.lastname: Collins
authors.affiliation: Núcleo de Pesquisa e Inovação em Ciências da Saúde (NUPICS), Federal University of Juiz de Fora, 36036-900 Juiz de Fora, MG, Brazil. Electronic address: marcosbrand2012@gmail.com.
authors.forename: Marcos Antônio Fernandes
authors.initials: MA
authors.lastname: Brandão
authors.affiliation: Núcleo de Pesquisa e Inovação em Ciências da Saúde (NUPICS), Federal University of Juiz de Fora, 36036-900 Juiz de Fora, MG, Brazil. Electronic address: nadiafox@gmail.com.
authors.forename: Nádia Rezende Barbosa
authors.initials: NR
authors.lastname: Raposo
mesh_terms: D000279:Administration, Cutaneous; D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005260:Female; D006801:Humans; D010539:Permeability; D000077185:Resveratrol; D012822:Silicon Dioxide / Q000737:chemistry; D012869:Skin Absorption; D063465:Skin Cream / Q000008:administration & dosage / Q000493:pharmacokinetics; D013267:Stilbenes / Q000008:administration & dosage / Q000493:pharmacokinetics*
chemical_list: D013267:Stilbenes; D012822:Silicon Dioxide; D000077185:Resveratrol
keywords: Factorial design; In vitro drug release; Percutaneous absorption; Resveratrol; Transdermal dosage form; Validation studies