title: Mechanism and manipulation of DNA:RNA hybrid G-quadruplex formation in transcription of G-rich DNA.
abstract: We recently reported that a DNA:RNA hybrid G-quadruplex (HQ) forms during transcription of DNA that bears two or more tandem guanine tracts (G-tract) on the nontemplate strand. Putative HQ-forming sequences are enriched in the nearby 1000 nt region right downstream of transcription start sites in the nontemplate strand of warm-blooded animals, and HQ regulates transcription under both in vitro and in vivo conditions. Therefore, knowledge of the mechanism of HQ formation is important for understanding the biological function of HQ as well as for manipulating gene expression by targeting HQ. In this work, we studied the mechanism of HQ formation using an in vitro T7 transcription model. We show that RNA synthesis initially produces an R-loop, a DNA:RNA heteroduplex formed by a nascent RNA transcript and the template DNA strand. In the following round of transcription, the RNA in the R-loop is displaced, releasing the RNA in single-stranded form (ssRNA). Then the G-tracts in the RNA can jointly form HQ with those in the nontemplate DNA strand. We demonstrate that the structural cascade R-loop → ssRNA → HQ offers opportunities to intercept HQ formation, which may provide a potential method to manipulate gene expression.
authors.affiliation: State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences , Beijing 100101, People's Republic of China.
authors.forename: Jia-yu
authors.initials: JY
authors.lastname: Zhang
authors.forename: Ke-wei
authors.initials: KW
authors.lastname: Zheng
authors.forename: Shan
authors.initials: S
authors.lastname: Xiao
authors.forename: Yu-hua
authors.initials: YH
authors.lastname: Hao
authors.forename: Zheng
authors.initials: Z
authors.lastname: Tan
mesh_terms: D004247:DNA / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D054856:G-Quadruplexes; D006147:Guanine / Q000737:chemistry* / Q000378:metabolism; D012313:RNA / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012260:Ribonucleases / Q000737:chemistry / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D006147:Guanine; D012313:RNA; D004247:DNA; D012260:Ribonucleases