title: Four cDNAs encoding lipoprotein receptors from shrimp (Pandalopsis japonica): structural characterization and expression analysis during maturation.
abstract: As in all other oviparous animals, lipoprotein receptors play a critical role in lipid metabolism and reproduction in decapod crustaceans. Four full-length cDNAs encoding lipoprotein receptors (Paj-VgR, Paj-LpR1, Paj-LpR2A, and Paj-LpR2B) were identified from Pandalopsis japonica through a combination of EST screening and PCR-based cloning. Paj-LpR1 appears to be the first crustacean ortholog of insect lipophorin receptors, and its two paralogs, Paj-LpR2A and Paj-LpR2B, exhibited similar structural characteristics. Several transcriptional isoforms were also identified for all three Paj-LpRs. Each expression pattern was unique, suggesting different physiological roles for these proteins. Paj-VgR is an ortholog of vitellogenin (Vg) receptors from other decapod crustaceans. A phylogenetic analysis of lipoproteins and their receptors suggested that the nomenclature of Vgs from decapod crustaceans may need to be changed. A PCR-based transcriptional analysis showed that Paj-VgR and Paj-LpR2B are expressed almost exclusively in the ovary, whereas Paj-LpR1 and Paj-LpR2A are expressed in multiple tissues. The various transcriptional isoforms of the three Paj-LpRs exhibited unique tissue distribution profiles. A transcriptional analysis of each receptor using tissues with different GSI values showed that the change in transcription of Paj-VgRs, Paj-LpR2A and Paj-LpR1 was not as significant as that of Vgs during maturation. However, the transcriptional levels of Paj-LpR2B decreased in ovary at maturation, suggesting that their transcriptional regulation is involved in reproduction.
authors.affiliation: Interdisciplinary program of Biomedical Engineering, Pukyong National University, Busan, 608-737, South Korea.
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Interdisciplinary program of Biomedical Engineering, Pukyong National University, Busan, 608-737, South Korea.
authors.forename: Bo Kwang
authors.initials: BK
authors.lastname: Kim
authors.affiliation: Fisheries Resources Research Division, National Fisheries Research and Development Institute, Busan, 619-902, South Korea.
authors.forename: Young-Il
authors.initials: YI
authors.lastname: Seo
authors.affiliation: Fisheries Resources Research Division, National Fisheries Research and Development Institute, Busan, 619-902, South Korea.
authors.forename: Jung Hwa
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Gyeongsangnam-do Fisheries Resources Research Institute, South Korea.
authors.forename: Seung-Wan
authors.initials: SW
authors.lastname: Kang
authors.affiliation: POSTECH Ocean Science and Technology Institute, Pohang University of Science and Technology (POSTECH), Pohang 790-784, South Korea.
authors.forename: Chang-Keun
authors.initials: CK
authors.lastname: Kang
authors.affiliation: Department of Marine Biology, Pukyong National University, Busan 608-737, South Korea.
authors.forename: Won-gyu
authors.initials: WG
authors.lastname: Park
authors.affiliation: Department of Marine Biology, Pukyong National University, Busan 608-737, South Korea; Interdisciplinary program of Biomedical Engineering, Pukyong National University, Busan, 608-737, South Korea. Electronic address: kimhw@pknu.ac.kr.
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005260:Female; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010053:Ovary / Q000378:metabolism; D033561:Penaeidae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D018160:Receptors, Cytoplasmic and Nuclear; C432945:lipophorin receptor
keywords: Decapod; Lipophorin; Pandalopsis japonica; Reproduction; Vitellogenin