title: Genome expression analysis by suppression subtractive hybridization identified overexpression of Humanin, a target gene in gastric cancer chemoresistance.
abstract: BACKGROUND
  In cancer cells, apoptosis is an important mechanism that influences the outcome of chemotherapy and the development of chemoresistance. To find the genes involved in chemoresistance and the development of gastric cancer, we used the suppression subtractive hybridization method to identify the genes that are overexpressed in gastric cancer tissues compared to normal gastric tissues.
  
  
  RESULTS
  In the suppression subtractive hybridization library we constructed, the most highly overexpressed genes were humanin isoforms. Humanin is a recently identified endogenous peptide that has anti-apoptotic activity and has been selected for further study due to its potential role in the chemoresistance of gastric cancer. Upregulation of humanin isoforms was also observed in clinical samples by using quantitative real-time PCR. Among the studied isoforms, humanin isoform 3, with an expression level of 4.166 ± 1.44 fold, was the most overexpressed isoform in GC.
  
  
  CONCLUSIONS
  The overexpression of humanin in gastric cancer suggests a role for chemoresistance and provides new insight into the biology of gastric cancer. We propose that humanin isoforms are novel targets for combating chemoresistance in gastric cancer.
authors.forename: Negar
authors.initials: N
authors.lastname: Mottaghi-Dastjerdi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Soltany-Rezaee-Rad
authors.forename: Zargham
authors.initials: Z
authors.lastname: Sepehrizadeh
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Roshandel
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Ebrahimifard
authors.affiliation: Department of Pharmaceutical Biotechnology and Pharmaceutical Biotechnology Research Center, School of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, Iran. nsetayesh@tums.ac.ir.
authors.forename: Neda
authors.initials: N
authors.lastname: Setayesh