title: Food matrix effects on bioaccessibility of β-carotene can be measured in an in vitro gastrointestinal model.
abstract: Since the food matrix determines β-carotene availability for intestinal absorption, food matrix effects on the bioaccessibility of β-carotene from two diets were investigated in vitro and compared with in vivo data. The "mixed diet" consisted of β-carotene-rich vegetables, and the "oil diet" contained β-carotene-low vegetables with supplemental β-carotene. The application of extrinsically labeled β-carotene was also investigated. The bioaccessibility of β-carotene was 28 μg/100 μg β-carotene from the mixed diet and 53 μg/100 μg β-carotene from the oil diet. This ratio of 1.9:1 was consistent with in vivo data, where the apparent absorption was 1.9-fold higher in the oil diet than in the mixed diet. The labeled β-carotene was not equally distributed over time. In conclusion, the food matrix effects on bioaccessibility of β-carotene could be measured using an in vitro model and were consistent with in vivo data. The application of extrinsically labeled β-carotene was not confirmed.
authors.affiliation: Department of Gastroenterology and Hepatology, Radboud University Nijmegen Medical Centre , Nijmegen, The Netherlands.
authors.forename: Carolien A
authors.initials: CA
authors.lastname: Van Loo-Bouwman
authors.forename: Ton H J
authors.initials: TH
authors.lastname: Naber
authors.forename: Mans
authors.initials: M
authors.lastname: Minekus
authors.forename: Richard B
authors.initials: RB
authors.lastname: van Breemen
authors.forename: Paul J M
authors.initials: PJ
authors.lastname: Hulshof
authors.forename: Gertjan
authors.initials: G
authors.lastname: Schaafsma
mesh_terms: D001682:Biological Availability; D004032:Diet; D004042:Dietary Fats, Unsaturated; D019587:Dietary Supplements; D004063:Digestion; D005502:Food; D041981:Gastrointestinal Tract / Q000378:metabolism; D066298:In Vitro Techniques; D008954:Models, Biological; D014675:Vegetables / Q000737:chemistry; D019207:beta Carotene / Q000008:administration & dosage / Q000493:pharmacokinetics*
chemical_list: D004042:Dietary Fats, Unsaturated; D019207:beta Carotene