title: Transferability of oxytetracycline (OTC) from feed to carp muscle and evaluation of the antibiotic effects on antioxidant systems in liver and kidney.
abstract: Oxytetracycline (OTC) is employed in fish farms to contest or prevent bacterial infections. We simulated an OTC treatment at therapeutic level (75 mg kg(-1)) and at higher doses (150, 300 mg kg(-1)) for 10 days. A withdrawal period of 10 days was considered for treated carp, carrying out the same chemical and biochemical analyses (total glutathione, superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione S-transferase and malondialdehyde). The aim was to obtain data related to the carryover in muscle and on variations in the antioxidant indicators in liver and kidney. The OTC residual levels in muscle showed a dose-response relationship. After 10 days of treatment at the recommended dose (75 mg kg(-1)), the mean value in muscle was 295 μg kg(-1). After 10 withdrawal days, residues in all treated groups were not entirely eliminated by fish. Residues of recommended 75 mg kg(-1) OTC dose were lower than the maximum permitted by EEC regulation: 100 μg kg(-1). Disturbance in the antioxidant systems in liver and kidney was recorded in (150, 300 mg kg(-1)) carp, as well as during the withdrawal period. A lowered superoxide dismutase activity and higher levels of catalase, glutathione peroxidase, glutathione reductase and glutathione were evaluated in liver, while in kidney only higher malondialdehyde and glutathione S-transferase concentrations were recorded for 300 mg kg(-1) dose. The therapeutic OTC dose exerted lower effects, and only in liver, enhancement of GPx and GR activities was recorded. After the withdrawal period, altered antioxidant responses in tissues were restored for all three OTC doses.
authors.affiliation: Department of Cellular and Environmental Biology, University of Perugia, 06123, Perugia, Italy, antonia.elia@unipg.it.
authors.forename: Antonia Concetta
authors.initials: AC
authors.lastname: Elia
authors.forename: Valentina
authors.initials: V
authors.lastname: Ciccotelli
authors.forename: Nicole
authors.initials: N
authors.lastname: Pacini
authors.forename: Ambrosius Josef Martin
authors.initials: AJ
authors.lastname: Dörr
authors.forename: Marilena
authors.initials: M
authors.lastname: Gili
authors.forename: Mauro
authors.initials: M
authors.lastname: Natali
authors.forename: Laura
authors.initials: L
authors.lastname: Gasco
authors.forename: Marino
authors.initials: M
authors.lastname: Prearo
authors.forename: Maria Cesarina
authors.initials: MC
authors.lastname: Abete
mesh_terms: D000704:Analysis of Variance; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002347:Carps / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008315:Malondialdehyde / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D010118:Oxytetracycline / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione; D010118:Oxytetracycline