title: Development of an antigen-DNAzyme based probe for a direct antibody-antigen assay using the intrinsic DNAzyme activity of a daunomycin aptamer.
abstract: G-Quadruplex (G-4) structures are formed when G-rich DNA sequences fold into intra- or intermolecular four-stranded structures in the presence of metal ions. G-4-hemin complexes are often effective peroxidase-mimicking DNAzymes that are applied in many detection systems. This work reports the application of a G-rich daunomycin-specific aptamer for the development of an antibody-antigen detection assay. We investigated the ability of the daunomycin aptamer to efficiently catalyze the hemin-dependent peroxidase activity independent of daunomycin. A reporter probe consisting of biotinylated antigen and daunomycin aptamer coupled to streptavidin gold nanoparticles was successfully used to generate a colorimetric readout. In conclusion, the daunomycin aptamer can function as a robust alternative DNAzyme for the development of colorimetric assays.
authors.affiliation: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia. mimiez_m5@yahoo.com.my.
authors.forename: Noorsharmimi
authors.initials: N
authors.lastname: Omar
authors.affiliation: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia. qiutingloh@gmail.com.
authors.forename: Qiuting
authors.initials: Q
authors.lastname: Loh
authors.affiliation: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia. geejun@usm.my.
authors.forename: Gee Jun
authors.initials: GJ
authors.lastname: Tye
authors.affiliation: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia. yeesiew@usm.my.
authors.forename: Yee Siew
authors.initials: YS
authors.lastname: Choong
authors.affiliation: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia. rahmah@usm.my.
authors.forename: Rahmah
authors.initials: R
authors.lastname: Noordin
authors.affiliation: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia. gloekler@th-wildau.de.
authors.forename: Jörn
authors.initials: J
authors.lastname: Glökler
authors.affiliation: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia. theamsoon@usm.my.
authors.forename: Theam Soon
authors.initials: TS
authors.lastname: Lim
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry / Q000379:methods*; D021881:DNA, Catalytic / Q000737:chemistry*; D003630:Daunorubicin / Q000737:chemistry*
chemical_list: D021881:DNA, Catalytic; D003630:Daunorubicin