title: An in vitro test of the efficacy of silver-containing wound dressings against Staphylococcus aureus and Pseudomonas aeruginosa in simulated wound fluid.
abstract: An isothermal microcalorimetric assay was used to quantify the efficacy of a silver-containing wound dressing against two common wound pathogens, Pseudomonas aeruginosa and Staphylococcus aureus. The growth patterns of the two species were unique and varied depending on the environment in which the organisms were grown. Addition of non-silver-containing dressing altered the growth kinetics while addition of silver (contained either in a dressing or as AgNO3 solution) was seen to elicit inhibition and/or kill depending on concentration. Tests were conducted in nutrient broth and simulated wound fluid. It was found that minimum inhibitory and minimum bactericidal concentration values were higher in simulated wound fluid and under anaerobic conditions. Bioavailability of silver from the wound dressing was 35% against S. aureus in nutrient broth and 68% against both species in simulated wound fluid. The data highlight the importance of developing and conducting in vitro assays in biorelevant media.
authors.affiliation: UCL School of Pharmacy, University College London, 29-39 Brunswick Square, London WC1N 1AX, UK.
authors.forename: Jawal
authors.initials: J
authors.lastname: Said
authors.affiliation: UCL School of Pharmacy, University College London, 29-39 Brunswick Square, London WC1N 1AX, UK.
authors.forename: Cornelius C
authors.initials: CC
authors.lastname: Dodoo
authors.affiliation: ConvaTec GDC, First Avenue, Deeside Industrial Park, Deeside, Flintshire CH5 2NU, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Walker
authors.affiliation: ConvaTec GDC, First Avenue, Deeside Industrial Park, Deeside, Flintshire CH5 2NU, UK.
authors.forename: David
authors.initials: D
authors.lastname: Parsons
authors.affiliation: UCL School of Pharmacy, University College London, 29-39 Brunswick Square, London WC1N 1AX, UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Stapleton
authors.affiliation: UCL School of Pharmacy, University College London, 29-39 Brunswick Square, London WC1N 1AX, UK.
authors.forename: Anthony E
authors.initials: AE
authors.lastname: Beezer
authors.affiliation: UCL School of Pharmacy, University College London, 29-39 Brunswick Square, London WC1N 1AX, UK. Electronic address: s.gaisford@ucl.ac.uk.
authors.forename: Simon
authors.initials: S
authors.lastname: Gaisford
mesh_terms: D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D001458:Bandages; D001682:Biological Availability; D002151:Calorimetry; D008826:Microbial Sensitivity Tests; D011552:Pseudomonas Infections / Q000188:drug therapy; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D018030:Silver Compounds / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D012835:Silver Nitrate / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D013203:Staphylococcal Infections / Q000188:drug therapy; D013211:Staphylococcus aureus / Q000187:drug effects*; D014946:Wound Infection / Q000188:drug therapy* / Q000382:microbiology
chemical_list: D000891:Anti-Infective Agents, Local; D018030:Silver Compounds; D012835:Silver Nitrate
keywords: Isothermal calorimetry; Pseudomonas aeruginosa; Silver, Efficacy; Staphylococcus aureus; Wound dressings