title: Covariance spectroscopy in high-resolution multi-dimensional solid-state NMR.
abstract: Covariance spectroscopy (COV), a statistical method that provides increased sensitivity, can be applied to two-dimensional high-resolution solid-state NMR experiments, such as homonuclear spin-exchange spectroscopy. We the alternative States sampling scheme to the experimental time by 50%. By combining COV with other processing methods for non-uniform sampling (NUS), many different three-dimensional experiments can be performed with substantial increases in overall sensitivity. As an example, we show a three-dimensional homonuclear spin-exchange/separated-local-field (SLF) spectrum that enables the assignment of resonances and the measurement of structural restraints from a single experiment performed in a limited amount of time.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093-0307, USA.
authors.forename: Eugene C
authors.initials: EC
authors.lastname: Lin
authors.affiliation: Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093-0307, USA. Electronic address: sopella@ucsd.edu.
authors.forename: Stanley J
authors.initials: SJ
authors.lastname: Opella
mesh_terms: D000465:Algorithms; D025561:Bacteriophage Pf1 / Q000737:chemistry; D036022:Capsid Proteins / Q000737:chemistry; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D015203:Reproducibility of Results; D012815:Signal Processing, Computer-Assisted; D059629:Signal-To-Noise Ratio
chemical_list: D036022:Capsid Proteins; D009587:Nitrogen Isotopes