title: 1,25(OH)2D3 suppresses COX-2 up-regulation and thromboxane production in placental trophoblast cells in response to hypoxic stimulation.
abstract: In this study, we determined if vitamin D could inhibit oxidative stress-induced thromboxane production by placental trophoblasts. Trophoblast isolated from normal placentas were stimulated with CoCl2, a hypoxic mimicking agent, with or without pretreatment of 1,25(OH)2D3. Soluble phospholipase-A2, metabolites of thromboxane-A2 and prostacyclin, and 8-isoprostane were measured. Expression of cyclooxygenase-1 (COX-1), COX-2, and heme oxygenase-1 (HO-1) were determined. We found that pretreatment of trophoblasts with 1,25(OH)2D3 significantly reduced 8-isoprostane and the ratio of thromboxane-A2 to prostacyclin production, and blocked COX-2 expression induced by CoCl2. These results provide evidence of the beneficial effects of vitamin D on placental trophoblasts.
authors.affiliation: Dept. of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, PO Box 33932, Shreveport, LA 71130, USA; Dept. of Obstetrics and Gynecology, First Hospital, Harbin Medical University, Harbin, China.
authors.forename: J
authors.initials: J
authors.lastname: Sun
authors.affiliation: Dept. of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, PO Box 33932, Shreveport, LA 71130, USA; Dept. of Obstetrics and Gynecology, First Hospital, Harbin Medical University, Harbin, China.
authors.forename: W
authors.initials: W
authors.lastname: Zhong
authors.affiliation: Dept. of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, PO Box 33932, Shreveport, LA 71130, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Dept. of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, PO Box 33932, Shreveport, LA 71130, USA.
authors.forename: L J
authors.initials: LJ
authors.lastname: Groome
authors.affiliation: Dept. of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, PO Box 33932, Shreveport, LA 71130, USA. Electronic address: ywang1@lsuhsc.edu.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
mesh_terms: D002117:Calcitriol / Q000494:pharmacology; D051545:Cyclooxygenase 1 / Q000096:biosynthesis*; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D005260:Female; D051547:Heme Oxygenase-1 / Q000096:biosynthesis*; D006801:Humans; D011247:Pregnancy; D013931:Thromboxanes; D014327:Trophoblasts / Q000187:drug effects / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D013931:Thromboxanes; D051547:Heme Oxygenase-1; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D002117:Calcitriol
keywords: COX-2; Thromboxane; Trophoblast; Vitamin D