title: Aging biofilm from a full-scale moving bed biofilm reactor: characterization and enzymatic treatment study.
abstract: Effective removal of aging biofilm deserves to receive more attention. This study aimed to characterized aging biofilm from a full-scale moving bed biofilm reactor treating pharmaceutical wastewater and evaluate the hydrolysis effects of biofilm by different enzymatic treatments. Results from FTIR and biochemical composition analyses showed that it was a predominately organic-based biofilm with the ratio of total protein (PN) to polysaccharide (PS) of 20.17. A reticular structure of extracellular polymeric matrix (EPM) with filamentous bacteria as the skeleton was observed on the basal layer through SEM-EDS test. Among the four commercial proteases and amylases from Genencor®, proteases were shown to have better performances than amylases either on the removal of MLSS and PN/MLSS or on DOC (i.e., dissolved organic carbon)/MLSS raising of biofilm pellets. Difference of dynamic fluorescence characteristics of dissolved organic matters after treated by the two proteases indicated distinguishing mechanisms of the treating process.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, Jiangsu, PR China; Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, School of Chemistry and Chemical Engineering, Huaiyin Normal University, Huai'an 223300, Jiangsu, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, Jiangsu, PR China. Electronic address: hqren@nju.edu.cn.
authors.forename: Hongqiang
authors.initials: H
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, Jiangsu, PR China.
authors.forename: Lili
authors.initials: L
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, Jiangsu, PR China.
authors.forename: Jinju
authors.initials: J
authors.lastname: Geng
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, Jiangsu, PR China.
authors.forename: Ke
authors.initials: K
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, Jiangsu, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000681:Amylases / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D019149:Bioreactors / Q000382:microbiology*; D002244:Carbon / Q000032:analysis; D009930:Organic Chemicals / Q000032:analysis; D010447:Peptide Hydrolases / Q000494:pharmacology*; D012722:Sewage / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D009930:Organic Chemicals; D012722:Sewage; D002244:Carbon; D000681:Amylases; D010447:Peptide Hydrolases
keywords: Aging biofilm; Characterization of aging biofilm; Commercial proteases and amylases; Excitation emission matrix (EEM) fluorescence spectra; Moving bed biofilm reactor