title: UFV-P2 as a member of the Luz24likevirus genus: a new overview on comparative functional genome analyses of the LUZ24-like phages.
abstract: BACKGROUND
  Phages infecting spoilage microorganisms have been considered as alternative biocontrol agents, and the study of their genomes is essential to their safe use in foods. UFV-P2 is a new Pseudomonas fluorescens-specific phage that has been tested for its ability to inhibit milk proteolysis.
  
  
  RESULTS
  The genome of the phage UFV-P2 is composed of bidirectional modules and presented 75 functionally predict ORFs, forming clusters of early and late transcription. Further genomic comparisons of Pseudomonas-specific phages showed that these viruses could be classified according to conserved segments that appear be free from genome rearrangements, called locally collinear blocks (LCBs). In addition, the genome organization of the phage UFV-P2 was shown to be similar to that of phages PaP3 and LUZ24 which have recently been classified as a Luz24likevirus.
  
  
  CONCLUSIONS
  We have presented the functional annotation of UFV-P2, a new Pseudomonas fluorescens phage. Based on structural genomic comparison and phylogenetic clustering, we suggest the classification of UFV-P2 in the Luz24likevirus genus, and present a set of shared locally collinear blocks as the genomic signature for this genus.
authors.forename: Monique R
authors.initials: MR
authors.lastname: Eller
authors.forename: Pedro M P
authors.initials: PM
authors.lastname: Vidigal
authors.forename: Rafael L
authors.initials: RL
authors.lastname: Salgado
authors.forename: Maura P
authors.initials: MP
authors.lastname: Alves
authors.forename: Roberto S
authors.initials: RS
authors.lastname: Dias
authors.forename: Cynthia C
authors.initials: CC
authors.lastname: da Silva
authors.forename: Antônio F
authors.initials: AF
authors.lastname: de Carvalho
authors.forename: Andrew
authors.initials: A
authors.lastname: Kropinski
authors.affiliation: Laboratory of Molecular Immunovirology, Federal University of Viçosa, Av, PH Rolfs, s/n, Campus da UFV, Viçosa, Minas Gerais 36570-000, Brazil. depaula@ufv.br.
authors.forename: Sérgio O
authors.initials: SO
authors.lastname: De Paula
mesh_terms: D017901:Bacteriophage P2 / Q000235:genetics; D001435:Bacteriophages / Q000145:classification* / Q000235:genetics*; D016000:Cluster Analysis; D019295:Computational Biology; D016679:Genome, Viral; D016366:Open Reading Frames; D010802:Phylogeny; D011551:Pseudomonas fluorescens / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins