title: Subthalamic nucleus high-frequency stimulation restores altered electrophysiological properties of cortical neurons in parkinsonian rat.
abstract: Electrophysiological recordings performed in parkinsonian patients and animal models have confirmed the occurrence of alterations in firing rate and pattern of basal ganglia neurons, but the outcome of these changes in thalamo-cortical networks remains unclear. Using rats rendered parkinsonian, we investigated, at a cellular level in vivo, the electrophysiological changes induced in the pyramidal cells of the motor cortex by the dopaminergic transmission interruption and further characterized the impact of high-frequency electrical stimulation of the subthalamic nucleus, a procedure alleviating parkinsonian symptoms. We provided evidence that a lesion restricted to the substantia nigra pars compacta resulted in a marked increase in the mean firing rate and bursting pattern of pyramidal neurons of the motor cortex. These alterations were underlain by changes of the electrical membranes properties of pyramidal cells including depolarized resting membrane potential and increased input resistance. The modifications induced by the dopaminergic loss were more pronounced in cortico-striatal than in cortico-subthalamic neurons. Furthermore, subthalamic nucleus high-frequency stimulation applied at parameters alleviating parkinsonian signs regularized the firing pattern of pyramidal cells and restored their electrical membrane properties.
authors.affiliation: Team Dynamic and Pathophysiology of Neuronal Networks, Center for Interdisciplinary Research in Biology (CIRB), CNRS UMR7241/INSERM U1050, Collège de France, Paris, France ; Département de Neurologie - Centre de Référence Maladie de Parkinson, Hôpital Pitié-Salpêtrière, Assistance Publique - Hôpitaux de Paris (APHP), Paris, France.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Degos
authors.affiliation: Team Dynamic and Pathophysiology of Neuronal Networks, Center for Interdisciplinary Research in Biology (CIRB), CNRS UMR7241/INSERM U1050, Collège de France, Paris, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Deniau
authors.affiliation: Université Pierre et Marie Curie-Paris 6, CNRS-UMR-7225, Centre de Recherche de l'Institut du Cerveau et de la Moelle Epinière, Paris, France.
authors.forename: Mario
authors.initials: M
authors.lastname: Chavez
authors.affiliation: Team Dynamic and Pathophysiology of Neuronal Networks, Center for Interdisciplinary Research in Biology (CIRB), CNRS UMR7241/INSERM U1050, Collège de France, Paris, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Maurice
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004298:Dopamine / Q000172:deficiency / Q000502:physiology; D004599:Electric Stimulation Therapy; D055724:Electrophysiological Phenomena; D009044:Motor Cortex / Q000503:physiopathology; D009407:Nerve Block; D020734:Parkinsonian Disorders / Q000503:physiopathology* / Q000628:therapy*; D017966:Pyramidal Cells / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013378:Substantia Nigra / Q000293:injuries / Q000503:physiopathology; D020531:Subthalamic Nucleus / Q000503:physiopathology*
chemical_list: D004298:Dopamine