title: In vitro effects of Musa x paradisiaca extracts on four developmental stages of Haemonchus contortus.
abstract: This study was carried out to evaluate the in vitro effect of Musa x paradisiaca stem and leaf against the parasitic nematode of small ruminants Haemonchus contortus. Three extracts (aqueous, methanolic and/or dichloromethane) of Musa x paradisiaca stem and leaf were tested in vitro on four developmental stages of H. contortus using egg hatch assay (EHA), larval development assay (LDA), L3 migration inhibition assay (LMI) and adult worm motility assay (AWM). The highly significant (P<0.0001) ability to stop larval development (inhibition >67% for each extract) and the negative effect of the dichloromethane extract of leaf on adult worm motility (43% of inhibition of motility after 24h of incubation) compared to the negative controls, suggest anthelmintic properties of Musa x paradisiaca stem and leaf against H. contortus. The active principles responsible for the activity could be secondary metabolites such as terpenoid and flavonoid compounds present in the leaf and stem of the plant.
authors.affiliation: INRA UR143, Unité de Recherches Zootechniques, Domaine Duclos, Prise d'Eau, F-97170 Petit-Bourg, Guadeloupe. Electronic address: Carine.Marie-Magdeleine@antilles.inra.fr.
authors.forename: C
authors.initials: C
authors.lastname: Marie-Magdeleine
authors.affiliation: UMR QUALITROP Laboratoire de Chimie des Substances Naturelles, Campus de Fouillole, UFR SEN, 97 159 Pointe-à-Pître CEDEX, Guadeloupe.
authors.forename: L
authors.initials: L
authors.lastname: Udino
authors.affiliation: INRA UR143, Unité de Recherches Zootechniques, Domaine Duclos, Prise d'Eau, F-97170 Petit-Bourg, Guadeloupe.
authors.forename: L
authors.initials: L
authors.lastname: Philibert
authors.affiliation: INRA UE1294, Plateforme tropicale d'expérimentation sur l'animal, Domaine Duclos, F-97170 Petit-Bourg, Guadeloupe.
authors.forename: B
authors.initials: B
authors.lastname: Bocage
authors.affiliation: INRA UR143, Unité de Recherches Zootechniques, Domaine Duclos, Prise d'Eau, F-97170 Petit-Bourg, Guadeloupe.
authors.forename: H
authors.initials: H
authors.lastname: Archimede
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D002855:Chromatography, Thin Layer; D004305:Dose-Response Relationship, Drug; D019430:Guadeloupe; D006189:Haemonchus / Q000254:growth & development*; D066298:In Vitro Techniques; D007814:Larva / Q000254:growth & development; D028521:Musa / Q000737:chemistry*; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D012044:Regression Analysis
chemical_list: D000871:Anthelmintics; D010936:Plant Extracts
keywords: Adult worm motility; Banana tree; Egg hatching; Gastro-intestinal nematodes; In vitro assay; L3 migration; Larval development