title: Resistin increases platelet P-selectin levels via p38 MAPK signal pathway.
abstract: Resistin, an adipokine associated with the metabolic syndrome, is believed to have a role in thrombotic conditions. This work analyses the effects of resistin on P-selectin expression using a combination of ex vivo human studies, in vivo animal models and in vitro cell cultures. Human platelets and vascular endothelial cells were incubated with resistin, with or without anti-Toll-like receptor 4 (TLR-4) or mitogen-activated protein kinases (MAPK) pathway inhibitors, whereas mice were treated with resistin infusion followed by analysis of P-selectin expression. Resistin increased both human and murine platelet P-selectin expression compared with controls (human: 48.02% ± 7.6% vs 35.12% ± 2.62%, p < 0.05; mouse: 8.17% ± 0.37% vs 4.44% ± 0.37%, p < 0.05), through the p38 MAPK pathway. In contrast, resistin had no effect on endothelial P-selectin production. We conclude that resistin induces platelet activation by increasing P-selectin expression through the p38 MAPK-dependent pathway. These data provide one mechanism for the prothrombotic state in individuals with the metabolic syndrome.
authors.affiliation: Department of ICU, The First Affiliated Hospital of Shantou University Medical College, Shantou, PR China.
authors.forename: Wenbing
authors.initials: W
authors.lastname: Qiu
authors.forename: Naping
authors.initials: N
authors.lastname: Chen
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Liyuan
authors.initials: L
authors.lastname: Zhuo
authors.forename: Xihong
authors.initials: X
authors.lastname: Wang
authors.forename: Dongming
authors.initials: D
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Jin
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D019007:P-Selectin / Q000378:metabolism*; D010766:Phosphorylation; D052243:Resistin / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D019007:P-Selectin; D052243:Resistin; D048051:p38 Mitogen-Activated Protein Kinases
keywords: P-selectin; Resistin; p38 MAPK; platelet