title: Association of loss of heterozygosity with shorter survival in primary glioblastoma patients.
abstract: Loss of heterozygosity (LOH) co-deletion 1p/19q, MGMT promoter methylation and/or IDH1 mutation generally signify a better prognosis for patients with glioma. However, the influence of 1p/19q co-deletion and the LOH on other chromosomes in primary glioblastoma on survival is still debatable. The aim of our study was to identify LOH on chromosomes 1p, 19q, 9p, 10q, 13q, and 17p, and evaluate their impact either alone or 1p/19q co-deletion or by groups of LOH on the overall survival of 42 primary glioblastoma patients without an oligodendroglial component. These patients were additionally molecularly characterized for EGFR amplification, IDH1 mutations and TP53 mutations. We assessed their influence on the overall survival of glioblastoma patients. LOH in at least one of the loci on all examined chromosomes was detected in 65% of cases and was significantly associated with shorter overall survival (hazard ratio 3.07; 95% CI: 1.29-7.31, p = 0.006). 1p/19q co-deletion was infrequent (7.14%) and had no impact on overall survival. Our results indicate that in primary glioblastoma a specific LOH group analysis may be important for the prognosis. LOH 1p/19q co-deletion is rare in glioblastoma without an oligodendroglial component and has no impact on patient survival.
authors.affiliation: Dorota Jesionek-Kupnicka, PhD, Department of Pathology, Chair of Oncology, Medical University of Lodz, Pomorska 251, 92-213 Lodz, Poland, fax +48 42 272 56 04, e-mail: dorota.jesionek-kupnicka@umed.lodz.pl.
authors.forename: D
authors.initials: D
authors.lastname: Jesionek-Kupnicka
authors.forename: M
authors.initials: M
authors.lastname: Szybka
authors.forename: P
authors.initials: P
authors.lastname: Potemski
authors.forename: D
authors.initials: D
authors.lastname: Kulczycka-Wojdala
authors.forename: D
authors.initials: D
authors.lastname: Jaskólski
authors.forename: M
authors.initials: M
authors.lastname: Bieńkowski
authors.forename: W
authors.initials: W
authors.lastname: Skowroński
authors.forename: W
authors.initials: W
authors.lastname: Papierz
authors.forename: R
authors.initials: R
authors.lastname: Kordek
authors.forename: I
authors.initials: I
authors.lastname: Zawlik
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D002872:Chromosome Deletion; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D066246:ErbB Receptors / Q000235:genetics; D005260:Female; D005909:Glioblastoma / Q000235:genetics* / Q000401:mortality; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000235:genetics; D053208:Kaplan-Meier Estimate; D019656:Loss of Heterozygosity / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D011379:Prognosis; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D055815:Young Adult
chemical_list: D004273:DNA, Neoplasm; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D007521:Isocitrate Dehydrogenase; C543588:IDH1 protein, human; C512478:EGFR protein, human; D066246:ErbB Receptors