title: Screening and identification of the nucleic acid aptamers in nasopharyngeal carcinoma.
abstract: To screen the nucleic acid aptamers of the EB virus-positive nasopharyngeal carcinoma cells, we used SELEX technology and synthesized in vitro a 78-nucleotide random DNA library. We used normal nasopharyngeal epithelial cells and EB virus-positive low differentiated nasopharyngeal carcinoma cells as target to conduct 10 cycles of screening, cloning, sequencing, and identification of the aptamers. The fluorescence produced by the combination of the sub-library and the target cells gained intensity gradually with the increase in the number of screening cycles, indicating elevated binding capacity. The cluster analysis showed that the aptamers can be divided into three families, with two of the families having the common conserved sequence. In this study, by screening nucleic acid aptamers for affinity and specificity, we established an initial aptamer library for EB virus-positive nasopharyngeal carcinoma cells.
authors.affiliation: First Affiliated Hospital of Nanchang University, Nanchang, China.
authors.forename: W-X
authors.initials: WX
authors.lastname: Chen
authors.forename: K-H
authors.initials: KH
authors.lastname: Zhang
authors.forename: X-S
authors.initials: XS
authors.lastname: Zou
authors.forename: Y-Q
authors.initials: YQ
authors.lastname: Chen
authors.forename: J-G
authors.initials: JG
authors.lastname: Li
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics*; D001483:Base Sequence; D002277:Carcinoma; D045744:Cell Line, Tumor; D020031:Epstein-Barr Virus Infections / Q000235:genetics; D015723:Gene Library; D004854:Herpesvirus 4, Human; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000821:virology; D052156:SELEX Aptamer Technique; D017422:Sequence Analysis, DNA
chemical_list: D052157:Aptamers, Nucleotide