title: TNFα-induced miR-130 resulted in adipocyte dysfunction during obesity-related inflammation.
abstract: Adipocytes are continuously stimulated by proinflammatory cytokines such as TNFα, which cause adipocyte dysfunction by facilitating the inflammatory response. Although miR-130 was reported to be an important regulator of adipogenesis by targeting PPARγ mRNA, little is known about the mechanisms regulating miR-130 expression during the proinflammatory response. Here, we examined miR-130 levels in white adipose tissue (WAT) from high-fat diet (HFD) mice and TNFα-stimulated adipocytes. Primary transcripts of miR-130 were increased after TNFα stimulation, indicating that induction of miR-130 during the pro-inflammatory response is regulated by a transcriptional event. A chromatin immunoprecipitation assay showed that p65 binding to the promoter regions of miR-130 was enhanced after TNFα treatment. Taken together, our findings suggest that induction of miR-130 by TNFα is responsible for adipocyte dysfunction.
authors.forename: Chongtae
authors.initials: C
authors.lastname: Kim
authors.forename: Heejin
authors.initials: H
authors.lastname: Lee
authors.forename: Yoon Mi
authors.initials: YM
authors.lastname: Cho
authors.forename: Oh-Joo
authors.initials: OJ
authors.lastname: Kwon
authors.forename: Wook
authors.initials: W
authors.lastname: Kim
authors.forename: Eun Kyung
authors.initials: EK
authors.lastname: Lee
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects; D007249:Inflammation / Q000209:etiology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D041681:NIH 3T3 Cells; D009765:Obesity / Q000150:complications / Q000209:etiology / Q000378:metabolism*; D047495:PPAR gamma / Q000378:metabolism; D014158:Transcription, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D015854:Up-Regulation
chemical_list: C582652:MIRN130 microRNA, mouse; D035683:MicroRNAs; D047495:PPAR gamma; D014409:Tumor Necrosis Factor-alpha