title: Virus-like particle vaccine confers protection against a lethal newcastle disease virus challenge in chickens and allows a strategy of differentiating infected from vaccinated animals.
abstract: In this study, we developed Newcastle disease virus (NDV) virus-like particles (VLPs) expressing NDV fusion (F) protein along with influenza virus matrix 1 (M1) protein using the insect cell expression system. Specific-pathogen-free chickens were immunized with oil emulsion NDV VLP vaccines containing increasing dosages of VLPs (0.4, 2, 10, or 50 μg of VLPs/0.5-ml dose). Three weeks after immunization, the immunogenicity of the NDV VLP vaccines was determined using a commercial enzyme-linked immunosorbent assay (ELISA) kit, and a lethal challenge using a highly virulent NDV strain was performed to evaluate the protective efficacy of the NDV VLP vaccines. NDV VLP vaccines elicited anti-NDV antibodies and provided protection against a lethal challenge in a dose-dependent manner. Although the VLP vaccines containing 0.4 and 2 μg of VLPs failed to achieve high levels of protection, a single immunization with NDV VLP vaccine containing 10 or 50 μg could fully protect chickens from a lethal challenge and greatly reduced challenge virus shedding. Furthermore, we could easily differentiate infected from vaccinated animals (DIVA) using the hemagglutination inhibition (HI) test. These results strongly suggest that utilization of NDV VLP vaccine in poultry species may be a promising strategy for the better control of NDV.
authors.affiliation: Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
authors.forename: Jae-Keun
authors.initials: JK
authors.lastname: Park
authors.forename: Dong-Hun
authors.initials: DH
authors.lastname: Lee
authors.forename: Seong-Su
authors.initials: SS
authors.lastname: Yuk
authors.forename: Erdene-Ochir
authors.initials: EO
authors.lastname: Tseren-Ochir
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Kwon
authors.forename: Jin-Yong
authors.initials: JY
authors.lastname: Noh
authors.forename: Byoung-Yoon
authors.initials: BY
authors.lastname: Kim
authors.forename: Soo-Won
authors.initials: SW
authors.lastname: Choi
authors.forename: Sang-Moo
authors.initials: SM
authors.lastname: Kang
authors.forename: Joong-Bok
authors.initials: JB
authors.lastname: Lee
authors.forename: Seung-Yong
authors.initials: SY
authors.lastname: Park
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Choi
authors.forename: Chang-Seon
authors.initials: CS
authors.lastname: Song
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D009521:Newcastle Disease / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000276:immunology*; D016019:Survival Analysis; D014611:Vaccination / Q000379:methods; D022581:Vaccines, Marker / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D058425:Vaccines, Virus-Like Particle / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*; D017201:Virus Shedding
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; C488936:M1 protein, Influenza A virus; D022581:Vaccines, Marker; D058425:Vaccines, Virus-Like Particle; D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins; D014765:Viral Vaccines