title: Human mammospheres secrete hormone-regulated active extracellular vesicles.
abstract: Breast cancer is a leading cause of cancer-associated death worldwide. One of the most important prognostic factors for survival is the early detection of the disease. Recent studies indicate that extracellular vesicles may provide diagnostic information for cancer management. We demonstrate the secretion of extracellular vesicles by primary breast epithelial cells enriched for stem/progenitor cells cultured as mammospheres, in non-adherent conditions. Using a proteomic approach we identified proteins contained in these vesicles whose expression is affected by hormonal changes in the cellular environment. In addition, we showed that these vesicles are capable of promoting changes in expression levels of genes involved in epithelial-mesenchymal transition and stem cell markers. Our findings suggest that secreted extracellular vesicles could represent potential diagnostic and/or prognostic markers for breast cancer and support a role for extracellular vesicles in cancer progression.
authors.affiliation: Metabolomics CIBERehd, Derio, Spain.
authors.forename: Esperanza
authors.initials: E
authors.lastname: Gonzalez
authors.affiliation: Cell Biology and Stem Cells Unit, CIBERehd, Derio, Spain.
authors.forename: Marco
authors.initials: M
authors.lastname: Piva
authors.affiliation: Proteomics Platform, ProteoRed-ISCIII, CIBERehd, Derio, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Rodriguez-Suarez
authors.affiliation: Structural Biology, CIC bioGUNE, Derio, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Gil
authors.affiliation: Metabolomics CIBERehd, Derio, Spain.
authors.forename: Felix
authors.initials: F
authors.lastname: Royo
authors.affiliation: Proteomics Platform, ProteoRed-ISCIII, CIBERehd, Derio, Spain.
authors.forename: Felix
authors.initials: F
authors.lastname: Elortza
authors.affiliation: Metabolomics CIBERehd, Derio, Spain ; IKERBASQUE, Basque Foundation for Science, Bilbao, Spain.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Falcon-Perez
authors.affiliation: Cell Biology and Stem Cells Unit, CIBERehd, Derio, Spain.
authors.forename: Maria dM
authors.initials: Md
authors.lastname: Vivanco
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism; D045744:Cell Line, Tumor; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D019295:Computational Biology; D058750:Epithelial-Mesenchymal Transition; D005110:Extracellular Space / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006728:Hormones / Q000378:metabolism*; D006801:Humans; D042361:Mammary Glands, Human / Q000378:metabolism*; D014411:Neoplastic Stem Cells / Q000378:metabolism; D061251:Primary Cell Culture; D060066:Protein Interaction Maps; D020543:Proteome; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D018874:Spheroids, Cellular; D013629:Tamoxifen / Q000494:pharmacology
chemical_list: D006728:Hormones; D020543:Proteome; D013629:Tamoxifen