title: No microsatellite instability using Bethesda panel and revised markers in uterine leiomyomas.
abstract: Uterine leiomyomas are benign tumors of the uterus that arise clonally from smooth muscle cells of the myometrium and are very common reason for hysterectomy. The aim of this study was to evaluate microsatellite instability (MSI) in uterine leiomyomas using a set of MSI markers by Promega Corporation (Madison, WI, USA) and the Bethesda guideline. DNA was isolated from paired normal and tumoral tissues in 50 patients with uterine leiomyomas and MSI was analyzed by using seven microsatellite markers. Our result showed that microsatellite stability was found in all uterine leiomyomas. These data confirm the genetic status of uterine leiomyomas for the first time in Korean populations, and suggest that uterine leiomyomas have genetic stability in Korean.
authors.affiliation: Department of Biological Sciences, Dartmouth College, Hanover, NH, USA.
authors.forename: Ja-Min
authors.initials: JM
authors.lastname: Hong
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Gi-Jeong
authors.initials: GJ
authors.lastname: Gwon
authors.forename: Dae-Kwang
authors.initials: DK
authors.lastname: Kim
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D006801:Humans; D007889:Leiomyoma / Q000235:genetics*; D053842:Microsatellite Instability; D018895:Microsatellite Repeats; D008875:Middle Aged; D014594:Uterine Neoplasms / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm
keywords: Uterine leiomyomas; genetic instability; microsatellite instability