title: An improved method for determining virucidal efficacy of a chemical disinfectant using an electrical impedance assay.
abstract: A major problem with the testing of virucidal efficacy using current protocols is that scoring of virus-induced cytopathic effect (CPE) is dependent on subjective visual interpretation using light microscopy. The current report details the use of an electrical impedance assay (xCELLigence, ACEA Biosciences) for its utility in virucidal efficacy testing. In this study, the xCELLigence system was used in a procedure developed from guidelines given by the Deutsche Vereiniging zur Bekämpfung der Viruskrankheiten (DVV) (German Association for the Control of Virus Diseases) in order to demonstrate the inactivation of infectious bursal disease virus using a commercial virucide. Although the modified DVV assay using the xCELLigence system yielded identical results (i.e. a 5-log10 reduction in viral infectivity) as the traditional DVV assay, the system allows virucidal efficacy and cytotoxicity to be measured in a more precise and reproducible fashion.
authors.affiliation: Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa. Electronic address: karen.ebersohn@up.ac.za.
authors.forename: Karen
authors.initials: K
authors.lastname: Ebersohn
authors.affiliation: Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa.
authors.forename: Peter
authors.initials: P
authors.lastname: Coetzee
authors.affiliation: Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa.
authors.forename: E H
authors.initials: EH
authors.lastname: Venter
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D003584:Cytological Techniques / Q000379:methods*; D004202:Disinfectants / Q000494:pharmacology*; D017097:Electric Impedance; D007243:Infectious bursal disease virus / Q000187:drug effects* / Q000502:physiology; D050296:Microbial Viability / Q000187:drug effects*; D014709:Vero Cells; D038301:Virus Inactivation
chemical_list: D004202:Disinfectants
keywords: Cytopathic effect; Electrical impedance assay; Infectious bursal disease virus; Virucide efficacy