title: Protein tyrosine phosphatase 4A3 (PTP4A3) is required for Xenopus laevis cranial neural crest migration in vivo.
abstract: Uveal melanoma is the most common intraocular malignancy in adults, representing between about 4% and 5% of all melanomas. High expression levels of Protein Tyrosine Phosphatase 4A3, a dual phosphatase, is highly predictive of metastasis development and PTP4A3 overexpression in uveal melanoma cells increases their in vitro migration and in vivo invasiveness. Melanocytes, including uveal melanocytes, are derived from the neural crest during embryonic development. We therefore suggested that PTP4A3 function in uveal melanoma metastasis may be related to an embryonic role during neural crest cell migration. We show that PTP4A3 plays a role in cephalic neural crest development in Xenopus laevis. PTP4A3 loss of function resulted in a reduction of neural crest territory, whilst gain of function experiments increased neural crest territory. Isochronic graft experiments demonstrated that PTP4A3-depleted neural crest explants are unable to migrate in host embryos. Pharmacological inhibition of PTP4A3 on dissected neural crest cells significantly reduced their migration velocity in vitro. Our results demonstrate that PTP4A3 is required for cephalic neural crest migration in vivo during embryonic development.
authors.affiliation: Institut Curie, Research Division, Orsay, France ; CNRS UMR3347, Orsay, France ; INSERM U1021, Orsay, France ; Université Paris Sud, Orsay, France.
authors.forename: Selma
authors.initials: S
authors.lastname: Maacha
authors.affiliation: Institut Curie, Research Division, Orsay, France ; CNRS UMR3347, Orsay, France ; INSERM U1021, Orsay, France ; Université Paris Sud, Orsay, France ; Université Paris Diderot, Sorbonne Paris Cité, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Planque
authors.affiliation: Institut Curie, Research Division, Orsay, France ; CNRS UMR3347, Orsay, France ; INSERM U1021, Orsay, France ; Université Paris Sud, Orsay, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Laurent
authors.affiliation: Institut Curie, Research Division, Orsay, France ; CNRS UMR3347, Orsay, France ; INSERM U1021, Orsay, France ; Université Paris Sud, Orsay, France.
authors.forename: Caterina
authors.initials: C
authors.lastname: Pegoraro
authors.affiliation: Institut Curie, Research Division, Orsay, France ; CNRS UMR3347, Orsay, France ; INSERM U1021, Orsay, France ; Université Paris Sud, Orsay, France.
authors.forename: Océane
authors.initials: O
authors.lastname: Anezo
authors.affiliation: Institut Curie, Research Division, Orsay, France ; CNRS UMR3347, Orsay, France ; INSERM U1021, Orsay, France ; Université Paris Sud, Orsay, France.
authors.forename: Frédérique
authors.initials: F
authors.lastname: Maczkowiak
authors.affiliation: Institut Curie, Research Division, Orsay, France ; CNRS UMR3347, Orsay, France ; INSERM U1021, Orsay, France ; Université Paris Sud, Orsay, France.
authors.forename: Anne H
authors.initials: AH
authors.lastname: Monsoro-Burq
authors.affiliation: Institut Curie, Research Division, Orsay, France ; CNRS UMR3347, Orsay, France ; INSERM U1021, Orsay, France ; Université Paris Sud, Orsay, France.
authors.forename: Simon
authors.initials: S
authors.lastname: Saule
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D008545:Melanoma / Q000503:physiopathology; D009362:Neoplasm Metastasis / Q000503:physiopathology*; D009432:Neural Crest / Q000196:embryology*; D016133:Polymerase Chain Reaction; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000378:metabolism*; D012886:Skull / Q000166:cytology / Q000196:embryology*; D059008:Time-Lapse Imaging; D014604:Uveal Neoplasms / Q000503:physiopathology; D029867:Xenopus Proteins / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology*; D000098943:Uveal Melanoma
chemical_list: D017931:DNA Primers; D029867:Xenopus Proteins; C000592341:PTP4A3 protein, Xenopus; D017027:Protein Tyrosine Phosphatases