title: Effects of PTTG down-regulation on proliferation and metastasis of the SCL-1 cutaneous squamous cell carcinoma cell line.
abstract: OBJECTIVE
  To study effects of down-regulation of pituitary tumor-transforming gene (PTTG) on proliferation and metastasis ability of the SCL-1 cutaneous squamous cell carcinoma (CSCC) cell line and explore related mechanisms.
  
  
  METHODS
  SCL-1 cells were divided into 3 groups (untreated, siRNA control and PTTG siRNA). Cell proliferation assays were performed using a CCK-8 kit and proliferation and metastasis ability were analyzed using Boyden chambers. In addition, expression of MMP-2 and MMP-9 was detected by r-time qPCR and Western blotting.
  
  
  RESULTS
  Down-regulation of PTTG could markedly inhibit cell proliferation in SCL-1 cells, compared to untreated and control siRNA groups (P < 0.05). Real-time qPCR demonstrated that expression levels of PTTG, MMP-2 and MMP-9 in the PTTG siRNA group were 0.8%, 23.2% and 21.3% of untreated levels. Western blotting revealed that expression of PTTG, MMP-2 and MMP-9 proteins in the PTTG siRNA group was obviously down-regulated. The numbers of migrating cells (51.38 ± 4.71) in the PTTG siRNA group was obviously lower than that in untreated group (131.33 ± 6.12) and the control siRNA group (127.72 ± 5.20) (P < 0.05), suggesting that decrease of proliferation and metastasis ability mediated by PTTG knock-down may be closely correlated with down-regulation of MMP-2 and MMP-9 expression.
  
  
  CONCLUSIONS
  Inhibition of PTTG expression may be a new target for therapy of CSCC.
authors.affiliation: Department of Dermatovenereology, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, China E-mail : ZhongweiTiancn@yeah.net.
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Xia
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Fu
authors.forename: Su-Ling
authors.initials: SL
authors.lastname: Xu
authors.forename: Zhan-Guo
authors.initials: ZG
authors.lastname: Li
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.forename: Zhong-Wei
authors.initials: ZW
authors.lastname: Tian
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D009362:Neoplasm Metastasis; D064249:Securin / Q000235:genetics*; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D064249:Securin; C578002:pituitary tumor-transforming protein 1, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9