title: Two dimensional barcode-inspired automatic analysis for arrayed microfluidic immunoassays.
abstract: The usability of many high-throughput lab-on-a-chip devices in point-of-care applications is currently limited by the manual data acquisition and analysis process, which are labor intensive and time consuming. Based on our original design in the biochemical reactions, we proposed here a universal approach to perform automatic, fast, and robust analysis for high-throughput array-based microfluidic immunoassays. Inspired by two-dimensional (2D) barcodes, we incorporated asymmetric function patterns into a microfluidic array. These function patterns provide quantitative information on the characteristic dimensions of the microfluidic array, as well as mark its orientation and origin of coordinates. We used a computer program to perform automatic analysis for a high-throughput antigen/antibody interaction experiment in 10 s, which was more than 500 times faster than conventional manual processing. Our method is broadly applicable to many other microchannel-based immunoassays.
authors.affiliation: College of Engineering and School of Physics, Peking University, Beijing 100871, China ; National Center for Nanoscience and Technology, Beijing 100190, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Engineering Physics, Tsinghua University, Beijing 100084, China ; Key Laboratory of Particle & Radiation Imaging (Tsinghua University), Ministry of Education, Beijing 100084, China.
authors.forename: Lingbo
authors.initials: L
authors.lastname: Qiao
authors.affiliation: College of Engineering and School of Physics, Peking University, Beijing 100871, China.
authors.forename: Yunke
authors.initials: Y
authors.lastname: Ren
authors.affiliation: State Key Laboratory for Nonlinear Mechanics, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Xuwei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Engineering Physics, Tsinghua University, Beijing 100084, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Gao
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, China.
authors.forename: Yunfang
authors.initials: Y
authors.lastname: Tang
authors.affiliation: College of Engineering and School of Physics, Peking University, Beijing 100871, China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Jeff Xi
authors.affiliation: College of Engineering and School of Physics, Peking University, Beijing 100871, China.
authors.forename: Tzung-May
authors.initials: TM
authors.lastname: Fu
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, China.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang