title: Changes in the content of short, medium and long-chain fatty acids in isolated hepatocytes incubated in the presence of magnesium ions and/or ethanol.
abstract: Magnesium is one of the commonly used dietary supplements. Therefore, this study was to evaluate the content of short, medium and long-chain fatty acids and their esters in isolated rat hepatocytes induced by magnesium and/or ethanol. Isolation of hepatocytes was carried out by the Seglen's enzymatic method using collagenase. To thus prepared samples ethanol and/or MgCl2 solution were added, respectively, so that their concentrations were as follows: 150 mM/dm3 ethanol and/or 2 mM/dm3 MgCl2, 4 mM/dm3 MgCl2. The contents of short, medium and long-chain fatty acids and those of ester-bound acids were determined. The statistical evaluation of the experiment was made by comparing the area normalized for the analysed fatty acids in hepatocytes incubated for 5 h in the presence of the test substances. The effect of magnesium ions on the content of fatty acids and their esters in isolated hepatocytes incubated for 5 h depended on their concentration in the medium. A normalizing effect of magnesium ions on ethanol-induced changes in the content of C14-C17, C18-C20 and C21-C24 fatty acids was demonstrated. A normalizing effect of magnesium on ethanol-induced changes in the content of ester-bound fatty acids in hepatocytes was not confirmed.
authors.affiliation: Department of Human Nutrition, Faculty of Public Health, Silesian Medical University, Zabrze, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Grochowska-Niedworok
authors.affiliation: Department of Human Nutrition, Faculty of Public Health, Silesian Medical University, Zabrze, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Calyniuk
authors.affiliation: Department of Internal Diseases, Silesian Medical University, Bytom, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Nowakowska-Zajdel
authors.affiliation: Department of Internal Diseases, Silesian Medical University, Bytom, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Muc-Wierzgon'
mesh_terms: D000818:Animals; D000431:Ethanol / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis*; D022781:Hepatocytes / Q000737:chemistry / Q000187:drug effects*; D008274:Magnesium / Q000494:pharmacology*; D051381:Rats
chemical_list: D005227:Fatty Acids; D000431:Ethanol; D008274:Magnesium