title: Visual detection of DNA on paper chips.
abstract: On-site DNA analysis for diagnostic or forensic purposes is much anticipated in the future of molecular testing. Yet the challenges to achieve this goal remain large with rapid and inexpensive detection and visualization being key factors for any portable analysis system. We have developed a filter paper-based nucleic acid assay, which is able to identify and distinguish dog and human genomic and mitochondrial samples in a forensic setting. The filter paper material allows for transport by capillary force of the sample DNA through the detection surface, allowing the targets to hybridize specifically to their complementary capture sequences. Coupling micrometer-sized beads to DNA allows the results to be visualized by the naked eye, enabling instant, cost-efficient, and on-site detection, while eliminating the need for advanced expensive instrumentation.
authors.affiliation: Division of Gene Technology, School of Biotechnology, Science for Life Laboratory, Royal Institute of Technology (KTH) , SE-171 65 Solna, Sweden.
authors.forename: Yajing
authors.initials: Y
authors.lastname: Song
authors.forename: Péter
authors.initials: P
authors.lastname: Gyarmati
authors.forename: Ana Catarina
authors.initials: AC
authors.lastname: Araújo
authors.forename: Joakim
authors.initials: J
authors.lastname: Lundeberg
authors.forename: Harry
authors.initials: H
authors.lastname: Brumer
authors.forename: Patrik L
authors.initials: PL
authors.lastname: Ståhl
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D015342:DNA Probes / Q000737:chemistry; D004285:Dogs; D005374:Filtration / Q000295:instrumentation*; D044707:Forensic Sciences; D006801:Humans; D010209:Paper; D013045:Species Specificity; D013499:Surface Properties; D013997:Time Factors
chemical_list: D015342:DNA Probes; D004247:DNA