title: Acrylic acid surface-modified contact lens for the culture of limbal stem cells.
abstract: Surface treatment to a biomaterial surface has been shown to modify and help cell growth. Our aim was to determine the best surface-modified system for the treatment of limbal stem cell deficiency (LSCD), which would facilitate expansion of autologous limbal epithelial cells, while maintaining cultivated epithelial cells in a less differentiated state. Commercially available contact lenses (CLs) were variously surface modified by plasma polymerization with ratios of acrylic acid to octadiene tested at 100% acrylic acid, 50:50% acrylic acid:octadiene, and 100% octadiene to produce high-, mid-, and no-acid. X-ray photoelectron spectroscopy was used to analyze the chemical composition of the plasma polymer deposited layer. Limbal explants cultured on high acid-modified CLs outgrew more cells. Immunofluorescence and RT2-PCR array results indicated that a higher acrylic acid content can also help maintain progenitor cells during ex vivo expansion of epithelial cells. This study provides the first evidence for the ability of high acid-modified CLs to preserve the stemness and to be used as substrates for the culture of limbal cells in the treatment of LSCD.
authors.affiliation: 1 Eye Hospital, Harbin Medical University , Nangang District, Harbin, Heilongjiang Province, China .
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Karl David
authors.initials: KD
authors.lastname: Brown
authors.forename: Sue Peng
authors.initials: SP
authors.lastname: Lowe
authors.forename: Guei-Sheung
authors.initials: GS
authors.lastname: Liu
authors.forename: David
authors.initials: D
authors.lastname: Steele
authors.forename: Keren
authors.initials: K
authors.lastname: Abberton
authors.forename: Mark
authors.initials: M
authors.lastname: Daniell
mesh_terms: D000179:Acrylates / Q000494:pharmacology*; D000368:Aged; D019149:Bioreactors; D002478:Cells, Cultured; D003261:Contact Lenses; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D016850:Limbus Corneae / Q000166:cytology*; D008875:Middle Aged; D056951:Photoelectron Spectroscopy; D058626:Plasma Gases / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013499:Surface Properties
chemical_list: D000179:Acrylates; D058626:Plasma Gases; C036658:acrylic acid