title: Secondary metabolites isolation in natural products chemistry: comparison of two semipreparative chromatographic techniques (high pressure liquid chromatography and high performance thin-layer chromatography).
abstract: Chemical investigations on secondary metabolites in natural products chemistry require efficient isolation techniques for characterization purpose as well as for the evaluation of their biological properties. In the case of phytochemical studies, the performance of the techniques is critical (resolution and yield) since the products generally present a narrow range of polarity and physicochemical properties. Several techniques are currently available, but HPLC (preparative and semipreparative) is the most widely used. To compare the performance of semipreparative HPLC and HPTLC for the isolation of secondary metabolites in different types of extracts, we have chosen carvone from spearmint essential oil (Mentha spicata L.), resveratrol from Fallopia multiflora (Thunb.) Haraldson, and rosmarinic acid from rosemary (Rosmarinus officinalis L.) extracts. The comparison was based on the chromatographic separation, the purity and quantity of isolated compounds, the solvent consumption, the duration and the cost of the isolation operations. The results showed that semipreparative HPTLC can in some case offer some advantages over conventional semipreparative HPLC.
authors.affiliation: BotaniCert, Espace Jacques-Louis Lions, 4 Traverse Dupont, 06130 Grasse, France; Institut de Chimie de Nice, UMR 7272, Université Nice Sophia Antipolis, CNRS, Parc Valrose, 06108 Nice Cedex 2, France.
authors.forename: Thi Kieu Tiên
authors.initials: TK
authors.lastname: Do
authors.affiliation: BotaniCert, Espace Jacques-Louis Lions, 4 Traverse Dupont, 06130 Grasse, France.
authors.forename: Francis
authors.initials: F
authors.lastname: Hadji-Minaglou
authors.affiliation: Institut de Chimie de Nice, UMR 7272, Université Nice Sophia Antipolis, CNRS, Parc Valrose, 06108 Nice Cedex 2, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Antoniotti
authors.affiliation: Institut de Chimie de Nice, UMR 7272, Université Nice Sophia Antipolis, CNRS, Parc Valrose, 06108 Nice Cedex 2, France. Electronic address: xavier.fernandez@unice.fr.
authors.forename: Xavier
authors.initials: X
authors.lastname: Fernandez
mesh_terms: D001688:Biological Products / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D002934:Cinnamates / Q000302:isolation & purification; D053630:Depsides / Q000302:isolation & purification; D031337:Mentha spicata / Q000737:chemistry*; D009822:Oils, Volatile / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D028502:Polygonum / Q000737:chemistry*; D027542:Rosmarinus / Q000737:chemistry*; D000097123:Rosmarinic Acid
chemical_list: D001688:Biological Products; D002934:Cinnamates; D053630:Depsides; D009822:Oils, Volatile; D010936:Plant Extracts; D000097123:Rosmarinic Acid
keywords: Fallopia multiflora (Thunb.) Haraldson; Mentha spicata L.; Phytochemistry; Planar chromatography (TLC, HPTLC); Purification; Rosmarinus officinalis L.