title: Identification of differentially expressed genes in the digestive gland of manila clam Ruditapes philippinarum exposed to BDE-47.
abstract: Suppression subtractive hybridization (SSH) was used to identify alterations in gene transcription of the manila clam Ruditapes philippinarum after exposure to 5μg/L 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) for 15days. The ability to accumulate BDE-47 in digestive gland and gill was also evaluated in order to provide information for food safety. Analysis of tissue extracts indicated that digestive gland had the higher BDE-47 levels (12,463.1±1334.8 ng/g d.w.) compared to gill (6368.6±738.7ng/g d.w.) after a 15-day exposure period. Forward and reverse SSH libraries were made from pooled digestive glands of R. philippinarum, from which 75 high quality sequences were obtained by BLAST analysis. The expression of 39 genes with significant homology (E-value<10(-5)) out of the 75 sequences was investigated by quantitative RT-PCR. Among the 39 genes, 27 genes were found up-regulated while 12 genes were found down-regulated after the BDE-47 exposure. The 39 genes were involved in cellular cycle, cytoskeleton, substance and energy metabolism, stress response, innate immunity and cell signaling and transport which were extensively discussed. This study provides a preliminary basis for studying the response of marine bivalves upon exposure to PBDEs in terms of regulated gene expression.
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Miao
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Luqing
authors.initials: L
authors.lastname: Pan
authors.affiliation: Technical Center for Shandong Entry-exit Inspection and Quarantine Bureau, China.
authors.forename: Wenhao
authors.initials: W
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Yuefeng
authors.initials: Y
authors.lastname: Cai
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000187:drug effects* / Q000235:genetics*; D004064:Digestive System / Q000187:drug effects*; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D059022:Food Safety; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000235:genetics*; D005880:Gills / Q000187:drug effects; D055768:Halogenated Diphenyl Ethers / Q000506:poisoning*; D009693:Nucleic Acid Hybridization / Q000379:methods; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
chemical_list: D055768:Halogenated Diphenyl Ethers; C511295:2,2',4,4'-tetrabromodiphenyl ether
keywords: 2,2′,4,4′-Tetrabromodiphenyl ether; Differentially expressed genes; Ruditapes philippinarum; Suppression subtractive hybridization