title: NAD(P)H-independent asymmetric C=C bond reduction catalyzed by ene reductases by using artificial co-substrates as the hydrogen donor.
abstract: To develop a nicotinamide-independent single flavoenzyme system for the asymmetric bioreduction of C=C bonds, four types of hydrogen donor, encompassing more than 50 candidates, were investigated. Six highly potent, cheap, and commercially available co-substrates were identified that (under the optimized conditions) resulted in conversions and enantioselectivities comparable with, or even superior to, those obtained with traditional two-enzyme nicotinamide adenine dinucleotide phosphate (NAD(P)H)-recycling systems.
authors.affiliation: Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz (Austria), Fax: (+43) 316-380-9840.
authors.forename: Christoph K
authors.initials: CK
authors.lastname: Winkler
authors.forename: Dorina
authors.initials: D
authors.lastname: Clay
authors.forename: Marcello
authors.initials: M
authors.lastname: Entner
authors.forename: Markus
authors.initials: M
authors.lastname: Plank
authors.forename: Kurt
authors.initials: K
authors.lastname: Faber
mesh_terms: D055162:Biocatalysis; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002244:Carbon / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry*; D009243:NAD / Q000737:chemistry*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D001672:Biocompatible Materials; D009243:NAD; D002244:Carbon; D006859:Hydrogen; D010088:Oxidoreductases
keywords: alkene reduction; artificial biocatalysis; ene reductases; enzyme catalysis; hydrogen donors