title: Molecular typing of monophasic Salmonella 4,[5]:i:- strains isolated in Belgium (2008-2011).
abstract: To assess the distribution of Salmonella 4,[5]:i:- subtypes in the Belgian food chain and compare it to the subtypes associated with human infections, a molecular assessment was initiated. Two hundred fifty-three Salmonella isolates serotyped as 4,[5]:i:- during the period 2008-2011 in Belgium and originating from animal productions, food or human clinical samples were analysed by a specific duplex PCR. One hundred ninety-four isolates (76.7%) fit the profile of a S. Typhimurium monophasic variant as defined by the European Food Safety Authority. The other isolates possessed but did not express the phase II flagellin gene (23.3%). Multiple Locus Variable Number of Tandem Repeats Analysis (MLVA) revealed many but closely related profiles in the fljB-negative S. Typhimurium monophasic variant isolates. Some MLVA types were associated with both human and animal isolates but no unique source of human contamination could be demonstrated.
authors.affiliation: Veterinary & Agrochemical Research Centre, Foodborne Bacterial Zoonoses & Antibiotic Resistance Unit, Groeselenbergstr. 99, B-1180 Brussels, Belgium.
authors.forename: Cécile
authors.initials: C
authors.lastname: Boland
authors.affiliation: National Reference Centre for Salmonella and Shigella, Bacterial Diseases Division, Communicable and Infectious Diseases, Scientific Institute of Public Health, Wytsmanstreet 14, B-1050 Brussels, Belgium.
authors.forename: Sophie
authors.initials: S
authors.lastname: Bertrand
authors.affiliation: National Reference Centre for Salmonella and Shigella, Bacterial Diseases Division, Communicable and Infectious Diseases, Scientific Institute of Public Health, Wytsmanstreet 14, B-1050 Brussels, Belgium.
authors.forename: Wesley
authors.initials: W
authors.lastname: Mattheus
authors.affiliation: Scientific Service Food-borne Pathogens, Communicable and Infectious Diseases, Scientific Institute of Public Health, Wytsmanstreet 14, B-1050 Brussels, Belgium.
authors.forename: Katelijne
authors.initials: K
authors.lastname: Dierick
authors.affiliation: Veterinary & Agrochemical Research Centre, Foodborne Bacterial Zoonoses & Antibiotic Resistance Unit, Groeselenbergstr. 99, B-1180 Brussels, Belgium. Electronic address: piwat@coda-cerva.be.
authors.forename: Pierre
authors.initials: P
authors.lastname: Wattiau
mesh_terms: D000818:Animals; D001434:Bacteriophage Typing; D001530:Belgium; D005408:Flagellin / Q000235:genetics; D059022:Food Safety / Q000379:methods*; D006801:Humans; D058889:Molecular Typing; D016133:Polymerase Chain Reaction; D012475:Salmonella / Q000235:genetics; D012478:Salmonella Food Poisoning / Q000453:epidemiology / Q000382:microbiology* / Q000517:prevention & control; D012480:Salmonella Infections / Q000453:epidemiology / Q000382:microbiology* / Q000517:prevention & control; D012486:Salmonella typhimurium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D020080:Tandem Repeat Sequences
chemical_list: D005408:Flagellin
keywords: 4,[5]:i:-; MLVA; Monophasic; Salmonella; Typhimurium