title: In vitro screening of Fe2+-chelating effect by a Fenton's reaction-luminol chemiluminescence system.
abstract: In vitro screening of a Fe(2+) -chelating effect using a Fenton's reaction-luminol chemiluminescence (CL) system is described. The luminescence between the reactive oxygen species generated by the Fenton's reaction and luminol was decreased on capturing Fe(2+) using a chelator. The proposed method can prevent the consumption of expensive seed compounds (drug discovery candidates) owing to the high sensitivity of CL detection. Therefore, the assay could be performed using small volumes of sample solution (150 μL) at micromolar concentrations. After optimization of the screening conditions, the efficacies of conventional chelators such as ethylenediaminetetraacetic acid (EDTA), diethylentriaminepentaacetic acid (DETAPAC), deferoxamine, deferiprone and 1,10-phenanthroline were examined. EC50 values for these compounds (except 1,10-phenanthroline) were in the range 3.20 ± 0.87 to 9.57 ± 0.64 μM (n = 3). Rapid measurement of the Fe(2+)-chelating effect with an assay run time of a few minutes could be achieved using the proposed method. In addition, the specificity of the method was discussed.
authors.affiliation: Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8521, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Wada
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Komatsu
authors.forename: Rie
authors.initials: R
authors.lastname: Ikeda
authors.forename: Talal A
authors.initials: TA
authors.lastname: Aburjai
authors.forename: Suleiman M
authors.initials: SM
authors.lastname: Alkhalil
authors.forename: Naotaka
authors.initials: N
authors.lastname: Kuroda
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Nakashima
mesh_terms: D000077543:Deferiprone; D003676:Deferoxamine / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry*; D005296:Ferrous Compounds / Q000737:chemistry*; D007502:Iron Chelating Agents / Q000737:chemistry*; D049449:Luminescence; D008165:Luminol / Q000737:chemistry*; D015394:Molecular Structure; D010618:Phenanthrolines / Q000737:chemistry*; D011728:Pyridones / Q000737:chemistry*
chemical_list: D005296:Ferrous Compounds; D007502:Iron Chelating Agents; D010618:Phenanthrolines; D011728:Pyridones; D000077543:Deferiprone; D008165:Luminol; D004492:Edetic Acid; D003676:Deferoxamine; C025205:1,10-phenanthroline
keywords: Fe2+; Fenton's reaction; chelating effect; in vitro screening; luminol