title: Anti-inflammatory properties of gastric-derived Lactobacillus plantarum XB7 in the context of Helicobacter pylori infection.
abstract: BACKGROUND
  Helicobacter pylori colonization of the gastric epithelium induces interleukin-8 (IL-8) production and inflammation leading to host cell damage. We searched for gastric-derived Lactobacillus with the ability to suppress H. pylori-induced inflammation.
  
  
  METHODS
  Conditioned media from gastric-derived Lactobacillus spp. were tested for the ability to suppress H. pylori-induced IL-8 production in AGS gastric epithelial cells. IL-8 protein and mRNA levels were measured by ELISA and qPCR, respectively. The changes on host cell signaling pathway were analyzed by Western blotting and the anti-inflammatory effect was tested in a Sprague-Dawley rat model.
  
  
  RESULTS
  Conditioned media from L. salivarius B101, L. rhamnosus B103, and L. plantarum XB7 suppressed IL-8 production and IL-8 mRNA expression in H. pylori-induced AGS cells without inhibiting H. pylori growth. Conditioned media from LS-B101, LR-B103, and LP-XB7 suppressed the activation of NF-κB in AGS cells, while strain LP-XB7 also suppressed c-Jun activation. The anti-inflammatory effect of LP-XB7 was further assessed in vivo using a H. pylori-infected Sprague-Dawley rat model. Strain LP-XB7 contributed to a delay in the detection and colonization of H. pylori in rat stomachs, attenuated gastric inflammation, and ameliorated gastric histopathology. Additionally, the administration of LP-XB7 correlated with the suppression of TNF-α and CINC-1 in sera, and suppression of CINC-1 in the gastric mucosa of H. pylori-infected rats.
  
  
  CONCLUSIONS
  These results suggest that L. plantarum XB7 produces secreted factors capable of modulating inflammation during H. pylori infection, and this probiotic Lactobacillus strain shows promise as an adjunctive therapy for treating H. pylori-associated disease.
authors.affiliation: Interdisciplinary Program of Medical Microbiology, Graduate School, Chulalongkorn University, Bangkok, 10330, Thailand.
authors.forename: Thien
authors.initials: T
authors.lastname: Thiraworawong
authors.forename: Jennifer K
authors.initials: JK
authors.lastname: Spinler
authors.forename: Duangporn
authors.initials: D
authors.lastname: Werawatganon
authors.forename: Naruemon
authors.initials: N
authors.lastname: Klaikeaw
authors.forename: Susan F
authors.initials: SF
authors.lastname: Venable
authors.forename: James
authors.initials: J
authors.lastname: Versalovic
authors.forename: Somying
authors.initials: S
authors.lastname: Tumwasorn
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000276:immunology; D005260:Female; D005753:Gastric Mucosa / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D016481:Helicobacter Infections / Q000276:immunology / Q000628:therapy*; D016480:Helicobacter pylori / Q000276:immunology; D006801:Humans; D056747:Immunomodulation; D007249:Inflammation / Q000276:immunology* / Q000382:microbiology; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D048031:JNK Mitogen-Activated Protein Kinases / Q000096:biosynthesis; D048191:Lactiplantibacillus plantarum / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D019936:Probiotics / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D013270:Stomach / Q000382:microbiology / Q000473:pathology; D051996:Transcription Factor RelA / Q000096:biosynthesis
chemical_list: D000893:Anti-Inflammatory Agents; D017077:Culture Media, Conditioned; D016209:Interleukin-8; D012333:RNA, Messenger; C498145:Rela protein, rat; D051996:Transcription Factor RelA; D048031:JNK Mitogen-Activated Protein Kinases
keywords: Helicobacter pylori; Lactobacillus plantarum; Sprague-Dawley rat; interleukin-8; probiotic; stomach