title: Kupffer cells suppress hepatocarcinogenesis and metastasis in tumor orthotopic implanted Kunming mice.
abstract: In this research, we used GdCl3 (gadolinium chloride) to restrain the function of Kupffer cells and assessed effects on hepatocarcinogenesis and metastasis in the Kunming mouse. A 0.25% GdCl3 solution (10 mg/kg b.w.) was infused via the vena caudalis of each mouse 1 week before inoculation of H22 cells and was continued once per three days. Then we observed the follow indexes 3 weeks after injection of H22 cells: tumor weight, histologic characteristics of tumor tissue by light microscopy, ultramicrostructure of Kupffer cells under the electron microscope, distribution and number of Kupffer cells by histochemical staining, and TNF-α and IFN-γ levels in blood-serum and liver tissue by ELISA and RT-PCR. MMP-2 protein expression was tested by immunohistochemistry. The GdCl3 pretreatment had no effect on the quantity of Kupffer cells, but clearly restrained their functions, with decrease of TNF-α and IFN-γ levels and elevation of MMP2. Tumor immunity functions were markedly suppressed and tumor growth was accelerated with appearance of metastasis. Furthermore, survival time of trial mice was shortened.
authors.affiliation: Department of Hepatobiliary surgery, Traditonal Chinese Medicine Hospital of Dianjiang, Chongqing, China E-mail : zw68229@sina.com.
authors.forename: X Y
authors.initials: XY
authors.lastname: Li
authors.forename: M Y
authors.initials: MY
authors.lastname: Wang
authors.forename: J Y
authors.initials: JY
authors.lastname: Zhang
authors.forename: J Z
authors.initials: JZ
authors.lastname: Li
authors.forename: J P
authors.initials: JP
authors.lastname: Gong
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D063646:Carcinogenesis / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D005682:Gadolinium / Q000494:pharmacology; D007371:Interferon-gamma / Q000378:metabolism; D007728:Kupffer Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology*; D008113:Liver Neoplasms / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D051379:Mice; D009362:Neoplasm Metastasis / Q000503:physiopathology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D005682:Gadolinium; D020778:Matrix Metalloproteinase 2; C038958:gadolinium chloride