title: Effects of muscarinic acetylcholine 3 receptor(208-227) peptide immunization on autoimmune response in nonobese diabetic mice.
abstract: The second extracellular loop (LFWQYFVGKRTVPPGECFIQFLSEPTITFGTAI, aa 205-237) of muscarinic acetylcholine 3 receptor (M3R) has been reported to be an epitope for autoantibodies generated during certain autoimmune disorders, including Sjögren's syndrome (SS). Autoantibodies against M3R(228-237) have been shown to interfere with the function of M3R. However, few studies have been performed on the M3R(205-227) peptide of the second extracellular loop. In the current study, we sought to investigate the effect of M3R(208-227) peptide immunization on autoimmune response in NOD/LtJ mice. We synthesized the M3R(208-227) peptide and immunized NOD/LtJ mice to investigate whether peptide-specific antibodies could be generated and whether immunization would lead to changes in autoimmune response in NOD/LtJ mice. Our results demonstrate that the secretions of Th-1, Th-2, and Th-17 cytokines are downregulated and lymphocytic infiltration is improved in the salivary glands and lacrimal glands following immunization with M3R(208-227) peptide in NOD/LtJ mice, suggesting that peptide immunotherapy using the M3R(208-227) peptide may represent a potential therapeutic alternative.
authors.affiliation: Key Autoimmunity Lab of Inner Mongolia, Institution of Immunology and Rheumatism, Baotou Medical College, No. 41 Lin yin Road, Baotou 014010, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Autoimmunity Lab of Inner Mongolia, Institution of Immunology and Rheumatism, Baotou Medical College, No. 41 Lin yin Road, Baotou 014010, China.
authors.forename: Jinzhe
authors.initials: J
authors.lastname: Ju
authors.affiliation: Key Autoimmunity Lab of Inner Mongolia, Institution of Immunology and Rheumatism, Baotou Medical College, No. 41 Lin yin Road, Baotou 014010, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Key Autoimmunity Lab of Inner Mongolia, Institution of Immunology and Rheumatism, Baotou Medical College, No. 41 Lin yin Road, Baotou 014010, China.
authors.forename: Fengfeng
authors.initials: F
authors.lastname: Lv
authors.affiliation: Key Autoimmunity Lab of Inner Mongolia, Institution of Immunology and Rheumatism, Baotou Medical College, No. 41 Lin yin Road, Baotou 014010, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Pang
authors.affiliation: Key Autoimmunity Lab of Inner Mongolia, Institution of Immunology and Rheumatism, Baotou Medical College, No. 41 Lin yin Road, Baotou 014010, China.
authors.forename: Guoan
authors.initials: G
authors.lastname: Yang
authors.affiliation: Key Autoimmunity Lab of Inner Mongolia, Institution of Immunology and Rheumatism, Baotou Medical College, No. 41 Lin yin Road, Baotou 014010, China.
authors.forename: Yongfu
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology / Q000378:metabolism; D015551:Autoimmunity; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D004195:Disease Models, Animal; D000939:Epitopes / Q000276:immunology*; D005260:Female; D007765:Lacrimal Apparatus / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008214:Lymphocytes / Q000276:immunology / Q000473:pathology; D051379:Mice; D016688:Mice, Inbred NOD; D010455:Peptides / Q000276:immunology*; D043587:Receptor, Muscarinic M3 / Q000737:chemistry / Q000276:immunology*; D012469:Salivary Glands / Q000276:immunology / Q000378:metabolism / Q000473:pathology
chemical_list: D000906:Antibodies; D001323:Autoantibodies; D016207:Cytokines; D000939:Epitopes; D010455:Peptides; D043587:Receptor, Muscarinic M3