title: Interleukin (IL)-21 promoter polymorphism increases the risk of thyroid cancer in Chinese population.
abstract: Polymorphisms in Interleukin (IL)-21 have been researched in several cancers, but the association between IL-21 polymorphisms and thyroid cancer remains unclarified. This case-control study explored the role of five tagSNPs (rs12508721C>T, rs907715G>A, rs13143866G>A, rs2221903A>G and rs4833837A>G) in IL-21 gene in thyroid cancer development. IL-21 genotypes were examined in 615 thyroid cancer patients and 600 controls in Chinese population, and the associations with the risk of thyroid cancer were estimated by logistic regression. Moreover, the potential role of rs12508721C>T in thyroid cancer was further explored by biochemical assays. Compared with the rs12508721CC genotype, CT genotype presented a significantly decreased risk of thyroid cancer (adjusted odds ratios [OR]=0.72; 95%CI=0.57-0.94), the TT carriers had a further decreased risk of thyroid cancer (OR=0.56; 95%CI=0.41-0.87). Furthermore, our quantitative real-time PCR and Enzyme-linked immunosorbent assay (ELISA) results demonstrated that the presence of rs12508721T allele led to more IL-21 expression. However, no significant difference was found in genotype frequencies for other four sites between cases and controls. These findings suggested that rs12508721 polymorphism in IL-21 might be a genetic modifier for the development of thyroid cancer.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery Sir Run Run Shaw Hospital, Key Laboratory of Biotherapy of Zhejiang Province, Zhejiang University, 3 Qinchun Road, Hangzhou 310006, China.
authors.forename: Mang
authors.initials: M
authors.lastname: Xiao
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery Sir Run Run Shaw Hospital, Key Laboratory of Biotherapy of Zhejiang Province, Zhejiang University, 3 Qinchun Road, Hangzhou 310006, China.
authors.forename: Sunhong
authors.initials: S
authors.lastname: Hu
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery Sir Run Run Shaw Hospital, Key Laboratory of Biotherapy of Zhejiang Province, Zhejiang University, 3 Qinchun Road, Hangzhou 310006, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Tang
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery Sir Run Run Shaw Hospital, Key Laboratory of Biotherapy of Zhejiang Province, Zhejiang University, 3 Qinchun Road, Hangzhou 310006, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Zhejiang Cancer Hospital, 38 Guangji Road, Hangzhou 310022, China. Electronic address: hf2000_jiang@126.com.
authors.forename: Huifen
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D007378:Interleukins / Q000097:blood / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D013964:Thyroid Neoplasms / Q000235:genetics*; D000098546:Interleukin-21
chemical_list: D007378:Interleukins; D000098546:Interleukin-21
keywords: CI; IL-21; MAF; OR; Polymorphisms; SNP; Thyroid cancer; confidence interval; minor allele frequency; odds ratio; single nucleotide polymorphism