title: Association between TNF-α and IL-1β genotypes vs Helicobacter pylori infection in Indonesia.
abstract: OBJECTIVE
  To investigate the correlation between the Helicobacter pylori (H. pylori) infection and host genetic background of healthy populations in Indonesia.
  
  
  METHODS
  In March 2007, epidemiological studies were undertaken on the general population of a city in Indonesia (Mataram, Lombok). The participants included 107 men and 187 women, whose ages ranged from 6 to 74 years old, with an average age of 34.0 (± 14.4) (± SD). The H. pylori of subject by UBT method determination, and through the polymerase chain reaction with confronting two-pair primers (PCR-CTPP) method parsing the single nucleotide polymorphism of interleukin (IL)-8, IL-4, IL-1β, CD14, tumor necrosis factor (TNF-α) and tyrosine-protein phosphates non-receptor type 11 (PTPN11) genotypes. The experimental data were analyzed by the statistical software SAS.
  
  
  RESULTS
  The H. pylori infection rates in the healthy Indonesian population studied were 8.4% for men and 12.8% for women; no obvious differences were noted for H. pylori infection rates by sex or age. TC genotypes of IL-4, TC and CC genotypes of TNF-α, and GA genotypes of PTPN11, were higher in frequency. Both CC and TC genotype of TNF-α T-1031C loci featured higher expressions in the healthy Indonesian population Indonesia studied of (OR = 1.99; 95%CI: 0.67-5.89) and (OR = 1.66; 95%CI: 0.73-3.76), respectively. C allele of IL-1β T-31C gene locus was at a higher risk (OR = 1.11; 95%CI: 0.70-1.73) of H. pylori infection, but no statistical significance was found in our study.
  
  
  CONCLUSIONS
  We reveal that the association between the TNF-α and IL-1β genotypes may be the susceptibility of H. pylori in the studied population.
authors.affiliation: Yang Zhao, Department of Cancer Center, Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Yang Zhao, Department of Cancer Center, Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China.
authors.forename: Jing-Wen
authors.initials: JW
authors.lastname: Wang
authors.affiliation: Yang Zhao, Department of Cancer Center, Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Yang Zhao, Department of Cancer Center, Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Hosono
authors.affiliation: Yang Zhao, Department of Cancer Center, Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Ando
authors.affiliation: Yang Zhao, Department of Cancer Center, Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China.
authors.forename: Shinkan
authors.initials: S
authors.lastname: Tokudome
authors.affiliation: Yang Zhao, Department of Cancer Center, Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China.
authors.lastname: Soeripto
authors.affiliation: Yang Zhao, Department of Cancer Center, Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China.
authors.forename: F X Ediati
authors.initials: FX
authors.lastname: Triningsih
authors.affiliation: Yang Zhao, Department of Cancer Center, Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China.
authors.forename: Tegu
authors.initials: T
authors.lastname: Triono
authors.affiliation: Yang Zhao, Department of Cancer Center, Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China.
authors.forename: Suwignyo
authors.initials: S
authors.lastname: Sumoharjo
authors.affiliation: Yang Zhao, Department of Cancer Center, Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China.
authors.forename: E Y Wenny Astuti
authors.initials: EY
authors.lastname: Achwan
authors.affiliation: Yang Zhao, Department of Cancer Center, Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China.
authors.forename: Stephanus
authors.initials: S
authors.lastname: Gunawan
authors.affiliation: Yang Zhao, Department of Cancer Center, Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China.
authors.forename: Yu-Min
authors.initials: YM
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016009:Chi-Square Distribution; D002648:Child; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D016481:Helicobacter Infections / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D016480:Helicobacter pylori / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions / Q000235:genetics; D006801:Humans; D007214:Indonesia / Q000453:epidemiology; D053583:Interleukin-1beta / Q000235:genetics*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D055815:Young Adult
chemical_list: D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha
keywords: Allele; Helicobacter pylori; Infection; Interleukin-1β; Tumor necrosis factor