title: Genetic diversity of Plasmodium vivax population in Anhui province of China.
abstract: BACKGROUND
  Although the numbers of malaria cases in China have been declining in recent years, outbreaks of Plasmodium vivax malaria were still being reported in rural areas south of the Yellow River. To better understand the transmission dynamics of P. vivax parasites in China, the extent of genetic diversity of P. vivax populations circulating in Bozhou of Anhui province of China were investigated using three polymorphic genetic markers: merozoite surface proteins 1 and 3α (pvmsp-1 and pvmsp-3α) and circumsporozoite protein (pvcsp).
  
  
  METHODS
  Forty-five P. vivax clinical isolates from Bouzhou of Anhui province were collected from 2009 to 2010 and were analysed using PCR/RFLP or DNA sequencing.
  
  
  RESULTS
  Seven and six distinct allelic variants were identified using PCR/RFLP analysis of pvmsp-3α with HhaI and AluI, respectively. DNA sequence analysis of pvmsp-1 (variable block 5) revealed that there were Sal-I and recombinant types but not Belem type, and seven distinct allelic variants in pvmsp-1 were detected, with recombinant subtype 2 (R2) being predominant (66.7%). All the isolates carried pvcsp with VK210 type but not VK247 or P. vivax-like types in the samples. Sequence analysis of pvcsp gene revealed 12 distinct allelic variants, with VK210-1 being predominant (41.5%).
  
  
  CONCLUSIONS
  The present data indicate that there is some degree of genetic diversity among P. vivax populations in Anhui province of China. The genetic data obtained may assist in the surveillance of P. vivax infection in endemic areas or in tracking potential future disease outbreak.
authors.forename: Bo
authors.initials: B
authors.lastname: Huang
authors.forename: Shiguang
authors.initials: S
authors.lastname: Huang
authors.forename: Xin-zhuan
authors.initials: XZ
authors.lastname: Su
authors.forename: Hong
authors.initials: H
authors.lastname: Guo
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Xu
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.forename: Xuchu
authors.initials: X
authors.lastname: Hu
authors.forename: Yaming
authors.initials: Y
authors.lastname: Yang
authors.forename: Shanqing
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, Guangdong, China. fanglilu@yahoo.com.
authors.forename: Fangli
authors.initials: F
authors.lastname: Lu
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000953:Antigens, Protozoan / Q000235:genetics / Q000378:metabolism; D002681:China / Q000453:epidemiology; D005819:Genetic Markers; D006801:Humans; D016780:Malaria, Vivax / Q000453:epidemiology* / Q000469:parasitology; D020066:Merozoite Surface Protein 1 / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D010966:Plasmodium vivax / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D000953:Antigens, Protozoan; D005819:Genetic Markers; D020066:Merozoite Surface Protein 1; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan; C089133:merozoite surface protein 3, Plasmodium