title: MicroRNA-34a induces apoptosis in the human glioma cell line, A172, through enhanced ROS production and NOX2 expression.
abstract: BACKGROUND
  MicroRNA is a type of non-coding small RNA involved in regulating genes and signaling pathways through incomplete complementation with target genes. Recent research supports key roles of miRNA in the formation and development of human glioma.
  
  
  METHODS
  The relative quantity of miR-34a was initially determined in human glioma A172 cells and glioma tissues. Next, we analyzed the impact of miR-34a on A172 cell viability with the MTT assay. The effects of miR-34a overexpression on apoptosis were confirmed with flow cytometry and Hoechst staining experiments. We further defined the target genes of miR-34a using immunofluorescence and Western blot.
  
  
  RESULTS
  MiR-34a expression was significantly reduced in human glioma A172 cells and glioma tissue, compared with normal glial cells and tissue samples. Our MTT data suggest that up-regulation of miR-34a inhibits cell viability while suppression of miR-34a enhances cell viability. Flow cytometry and Hoechst staining results revealed increased rates of apoptosis in A172 human glioma cells overexpressing miR-34a. Using immunofluorescence and Western blot analyses, we identified NOX2 as a target of miR-34a in A172 cells.
  
  
  CONCLUSIONS
  MiR-34a serves as a tumor suppressor in human glioma mainly by decreasing NOX2 expression.
authors.affiliation: Department of Neurosurgery, Xi-jing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: San-Zhong
authors.initials: SZ
authors.lastname: Li
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Medical Genetics and Developmental Biology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yi-Yang
authors.initials: YY
authors.lastname: Hu
authors.affiliation: Department of Anesthesiology, Xi-jing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, Xi-jing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yong-Bo
authors.initials: YB
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, Xi-jing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Ji-Dong
authors.initials: JD
authors.lastname: Sun
authors.affiliation: Department of Neurosurgery, Xi-jing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yue-Fan
authors.initials: YF
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, Xi-jing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Chen-Cheng
authors.initials: CC
authors.lastname: Ji
authors.affiliation: Department of Neurosurgery, Xi-jing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Zao-Bin
authors.initials: ZB
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Xi-jing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Cao
authors.affiliation: Department of Neurosurgery, Xi-jing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Qu
authors.affiliation: Department of Neurosurgery, Xi-jing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Xi-jing Hospital, Fourth Military Medical University, Xi'an 710032, China. Electronic address: chg16801@163.com.
authors.forename: Guang
authors.initials: G
authors.lastname: Cheng
authors.affiliation: Department of Neurosurgery, Xi-jing Hospital, Fourth Military Medical University, Xi'an 710032, China. Electronic address: zhoufei@fmmu.edu.cn.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Fei
mesh_terms: D017209:Apoptosis / Q000235:genetics* / Q000502:physiology; D045744:Cell Line, Tumor; D002470:Cell Survival; D016543:Central Nervous System Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D015536:Down-Regulation; D005910:Glioma / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: C522425:MIRN34 microRNA, human; D008562:Membrane Glycoproteins; D035683:MicroRNAs; D012334:RNA, Neoplasm; D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species; C056558:CYBB protein, human; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases
keywords: Apoptosis; Human glioma cell line A172; NOX2; miR-34a