title: Stress-induced phosphorylation of SNAP-25.
abstract: Synaptosomal-associated protein of 25 kDa (SNAP-25), a t-SNARE protein, plays a crucial role in neurotransmitter release by exocytosis. Protein kinase C phosphorylates SNAP-25 at Ser(187), however the physiological significance of this phosphorylation event in brain function remains unclear. In the present study, we found that SNAP-25 phosphorylation increased rapidly in the mouse brain following cold-water restraint stress. Both basal and stress-induced phosphorylation of SNAP-25 were high in stress-related brain regions, including the cerebral cortex, hippocampus, and amygdala, and the extent of phosphorylation increased with increasing amounts of stress. Intravenous administration of adrenaline increased SNAP-25 phosphorylation, although stress-induced phosphorylation was still observed in adrenalectomized mice. These results indicate that SNAP-25 phosphorylation is regulated in a stress-dependent manner through both central and peripheral mechanisms.
authors.affiliation: Department of Biochemistry, Kitasato University School of Medicine, Sagamihara, Kanagawa 252-0374, Japan. Electronic address: yamamori@med.kitasato-u.ac.jp.
authors.forename: Saori
authors.initials: S
authors.lastname: Yamamori
authors.affiliation: Department of Biochemistry, Kitasato University School of Medicine, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Daichi
authors.initials: D
authors.lastname: Sugaya
authors.affiliation: Department of Psychiatry, Kitasato University School of Medicine, Sagamihara, Kanagawa 252-0380, Japan.
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Iida
authors.affiliation: Department of Biochemistry, Kitasato University School of Medicine, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Kokubo
authors.affiliation: Department of Biochemistry, Kitasato University School of Medicine, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Itakura
authors.affiliation: International University of Health and Welfare Atami Hospital, Atami, Shizuoka 413-0012, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Suzuki
authors.affiliation: Department of Environmental Science and Technology, Faculty of Engineering, Shinshu University, Nagano-shi, Nagano 380-8553, Japan.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Kataoka
authors.affiliation: Department of Psychiatry, Kitasato University School of Medicine, Sagamihara, Kanagawa 252-0380, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Miyaoka
authors.affiliation: Department of Biochemistry, Kitasato University School of Medicine, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Masami
authors.initials: M
authors.lastname: Takahashi
mesh_terms: D000315:Adrenalectomy; D000818:Animals; D001921:Brain / Q000378:metabolism; D004837:Epinephrine / Q000494:pharmacology; D025461:Feedback, Physiological; D007103:Immobilization; D008297:Male; D008810:Mice, Inbred C57BL; D010766:Phosphorylation; D013315:Stress, Psychological / Q000378:metabolism* / Q000503:physiopathology; D013550:Swimming; D050825:Synaptosomal-Associated Protein 25 / Q000378:metabolism*
chemical_list: C494209:Snap25 protein, mouse; D050825:Synaptosomal-Associated Protein 25; D004837:Epinephrine
keywords: Exocytosis; Presynaptic regulation; Protein kinase C; SNARE protein