title: Effects of ω-3 polyunsaturated fatty acids on plasma proteome in Rett syndrome.
abstract: The mechanism of action of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) is only partially known. Prior reports suggest a partial rescue of clinical symptoms and oxidative stress (OS) alterations following ω -3 PUFAs supplementation in patients with Rett syndrome (RTT), a devastating neurodevelopmental disorder with transient autistic features, affecting almost exclusively females and mainly caused by sporadic mutations in the gene encoding the methyl CpG binding protein 2 (MeCP2) protein. Here, we tested the hypothesis that ω-3 PUFAs may modify the plasma proteome profile in typical RTT patients with MECP2 mutations and classic phenotype. A total of 24 RTT girls at different clinical stages were supplemented with ω-3 PUFAs as fish oil for 12 months and compared to matched healthy controls. The expression of 16 proteins, mainly related to acute phase response (APR), was changed at the baseline in the untreated patients. Following ω-3 PUFAs supplementation, the detected APR was partially rescued, with the expression of 10 out of 16 (62%) proteins being normalized. ω-3 PUFAs have a major impact on the modulation of the APR in RTT, thus providing new insights into the role of inflammation in autistic disorders and paving the way for novel therapeutic strategies.
authors.affiliation: Neonatal Intensive Care Unit, University Hospital Azienda Ospedaliera Universitaria Senese (AOUS), Viale M. Bracci 16, 53100 Siena, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: De Felice
authors.affiliation: Child Neuropsychiatry Unit, University Hospital AOUS, Viale M. Bracci 16, 53100 Siena, Italy ; Department of Medical Biotechnologies, University of Siena, Via A. Moro 2, 53100 Siena, Italy.
authors.forename: Alessio
authors.initials: A
authors.lastname: Cortelazzo
authors.affiliation: Department of Molecular and Developmental Medicine, University of Siena, Via A. Moro 2, 53100 Siena, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Signorini
authors.affiliation: Department of Medical Biotechnologies, University of Siena, Via A. Moro 2, 53100 Siena, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Guerranti
authors.affiliation: Child Neuropsychiatry Unit, University Hospital AOUS, Viale M. Bracci 16, 53100 Siena, Italy ; Department of Molecular and Developmental Medicine, University of Siena, Via A. Moro 2, 53100 Siena, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Leoncini
authors.affiliation: Child Neuropsychiatry Unit, University Hospital AOUS, Viale M. Bracci 16, 53100 Siena, Italy ; Department of Molecular and Developmental Medicine, University of Siena, Via A. Moro 2, 53100 Siena, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Pecorelli
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, CNRS/UM1/UM2/ENSCM, BP 14491, 34093 Montpellier Cedex 5, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Durand
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, CNRS/UM1/UM2/ENSCM, BP 14491, 34093 Montpellier Cedex 5, France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Galano
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, CNRS/UM1/UM2/ENSCM, BP 14491, 34093 Montpellier Cedex 5, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Oger
authors.affiliation: Child Neuropsychiatry Unit, University Hospital AOUS, Viale M. Bracci 16, 53100 Siena, Italy ; Department of Molecular and Developmental Medicine, University of Siena, Via A. Moro 2, 53100 Siena, Italy.
authors.forename: Gloria
authors.initials: G
authors.lastname: Zollo
authors.affiliation: Child Neuropsychiatry Unit, University Hospital AOUS, Viale M. Bracci 16, 53100 Siena, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Montomoli
authors.affiliation: Department of Life Science, University of Siena, Via A. Moro 2, 53100 Siena, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Landi
authors.affiliation: Department of Life Science, University of Siena, Via A. Moro 2, 53100 Siena, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Bini
authors.affiliation: Department of Life Sciences and Biotechnology, University of Ferrara, Via Borsari 46, 44100 Ferrara, Italy ; Department of Food and Nutrition, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Valacchi
authors.affiliation: Department of Molecular and Developmental Medicine, University of Siena, Via A. Moro 2, 53100 Siena, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Ciccoli
authors.affiliation: Child Neuropsychiatry Unit, University Hospital AOUS, Viale M. Bracci 16, 53100 Siena, Italy.
authors.forename: Joussef
authors.initials: J
authors.lastname: Hayek
mesh_terms: D000209:Acute-Phase Proteins / Q000032:analysis; D000293:Adolescent; D000328:Adult; D001798:Blood Proteins / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D019587:Dietary Supplements; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D006801:Humans; D016328:NF-kappa B / Q000502:physiology; D020543:Proteome; D015518:Rett Syndrome / Q000097:blood*
chemical_list: D000209:Acute-Phase Proteins; D001798:Blood Proteins; D015525:Fatty Acids, Omega-3; D016328:NF-kappa B; D020543:Proteome