title: [Effect of Chinese herbal medicine with Supplement Qi and Activating Blood Circulation on tubular reabsorption function of diabetic nephropathy rats].
abstract: OBJECTIVE
  To investigate the effect of Chinese herbal medicine with Supplement Qi and Activating Blood Circulation (huangqi and danshen) on urinary protein, kidney function and tubular reabsorption of diabetic nephropathy rats.
  
  
  METHODS
  SD rats were randomly divided into a nondiabetic control group (normal group) and three groups in which diabetes were induced by a single intraperitoneal injection of freshly prepared streptozotocin( STZ,55 mg/kg body weight). Then the diabetes rats were randomly assigned to three groups: diabetic model group, Supplement Qi and Activating Blood Circulation traditional Chinese medicine group (huangqi and danshen group) and Gliquidone group (as a reference hypoglycemic drug). Each group was treated with corresponding drugs for 6 weeks. At the end of the study, the rats from each group were injected with FITC-labeled BSA through tail vein. The 24 h urinary protein excretion were measured and blood was collected for measuring plasma glucose levels, serum creatinine (Cr), blood urea nitrogen (BUN), triglyceride (TG) and total cholesterol (T-CHO). Renal tissue was used to measure the level of LPO,SOD,GSH-Px and AGEs and Paraffin-embedded sections were stained with HE, PAS and immunohistochemistry.
  
  
  RESULTS
  The plasma glucose, the 24 h urinary protein excretion, the levels of serum Cr, BUN, TG and T-CHO in STZ-induced diabetic rats were higher than those of nondiabetic rats. Diabetic rats showed significantly increase in LPO and AGEs (P < 0.01) and decrease in antioxidant enzyme activity (both GSH-Px and SOD) (P < 0.05) as compared with non-diabetic control rats. Treatment with the Supplement Qi and Activating Blood Circulation traditional Chinese medicine for 6 weeks in diabetic rats significantly reduced the 24 h urinary protein excretion compared with model control (P < 0.01), and markedly decreased the levels of serum Cr,BUN,TG and T-CHO as compared with those of diabetic rat (P < 0.05). The levels of LPO and AGEs were decreased and the activity of GSH-Px was increased by Supplement Qi and Activating Blood Circulation treatment. The kidney proximal tubule lesions were improved and the reabsorption of FITC-BSA in tubular was increased in diabetic rats treated by huangqi and danshen, and the expression of megalin in proximal tubular was enhanced as compared with diabetic rats.
  
  
  CONCLUSIONS
  Diabetic nephropathy rats treated with traditional Chinese medicine therapeutic principles "Supplement Qi and Activating Blood Circulation" can reduce the 24 h urinary protein excretion and improve the function of tubular reabsorption. These protect effects may be in correlation with enhancement the renal tissue activity of antioxidant and up-regulation the expression of megalin in renal tubular epithelial cells in diabetic rats.
authors.affiliation: College of Pharmacy, Anhui University of Traditional Chinese Medicine/Engineering Technology Research Center of Modernized Chinese Material, Anhui Province, Hefei 230031, China. 23554351@qq.com
authors.forename: Juan-Juan
authors.initials: JJ
authors.lastname: Yin
authors.affiliation: College of Pharmacy, Anhui University of Traditional Chinese Medicine/Engineering Technology Research Center of Modernized Chinese Material, Anhui Province, Hefei 230031, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Clinical Laboratory of First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei 230038, China.
authors.forename: Qing-Bao
authors.initials: QB
authors.lastname: Wang
authors.affiliation: College of Pharmacy, Anhui University of Traditional Chinese Medicine/Engineering Technology Research Center of Modernized Chinese Material, Anhui Province, Hefei 230031, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Pharmacy, Anhui University of Traditional Chinese Medicine/Engineering Technology Research Center of Modernized Chinese Material, Anhui Province, Hefei 230031, China.
authors.forename: Deng-Ke
authors.initials: DK
authors.lastname: Yin
mesh_terms: D000042:Absorption / Q000187:drug effects; D000818:Animals; D027885:Astragalus propinquus / Q000737:chemistry; D015415:Biomarkers / Q000097:blood / Q000652:urine; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000188:drug therapy; D003928:Diabetic Nephropathies / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000627:therapeutic use*; D007677:Kidney Function Tests; D007684:Kidney Tubules / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D026561:Low Density Lipoprotein Receptor-Related Protein-2 / Q000378:metabolism*; D008297:Male; D008517:Phytotherapy / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza / Q000737:chemistry; D013311:Streptozocin
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D026561:Low Density Lipoprotein Receptor-Related Protein-2; D013311:Streptozocin