title: Glyceryl monooleate-based otic delivery system of ofloxacin: release profile and bactericidal activity.
abstract: This study investigated the preparation and characterization of glyceryl monooleate- (GMO) based drug delivery system containing ofloxacin for the treatment of otitis externa. Acetate buffer (pH 4.5) containing dissolved ofloxacin was added to molten GMO as an aqueous phase, this resulted in the formation of a cubic and a reverse hexagonal phases. The release behavior of ofloxacin from the drug delivery system was studied using three different methods. The mechanism of drug release using paddles/dissolution apparatus and Franz diffusion cells followed Higuchi and Fickian diffusion models; whereas intrinsic release rate method showed zero-order kinetics. The intrinsic release rate was estimated and found to be 187.2 µg/cm(2)/h. The release mechanisms were similar irrespective of the loaded ofloxacin amount, however, the higher drug load displayed higher release rate. The drug delivery system was proven to be microbiologically effective by using agar diffusion method, against Staphylococcus aureus, and Pseudomonas aeruginosa. The GMO/ofloxacin formulation was stable for 6 months after preparation at room temperature as measured with respect to phase stability and antibacterial activity.
authors.affiliation: Faculty of Pharmacy, The University of Jordan , Amman , Jordan and.
authors.forename: Samer
authors.initials: S
authors.lastname: Adwan
authors.forename: Rana
authors.initials: R
authors.lastname: Abu-Dahab
authors.forename: Amal G
authors.initials: AG
authors.lastname: Al-Bakri
authors.forename: Alsayyed
authors.initials: A
authors.lastname: Sallam
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004058:Diffusion; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D065546:Drug Liberation; D005989:Glycerides / Q000737:chemistry*; D015242:Ofloxacin / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012995:Solubility; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D005989:Glycerides; D015242:Ofloxacin; C005953:monoolein
keywords: Drug delivery; drug release; sustained release