title: The impact of bioaugmentation on dechlorination kinetics and on microbial dechlorinating communities in subsurface clay till.
abstract: A molecular study on how the abundance of the dechlorinating culture KB-1 affects dechlorination rates in clay till is presented. DNA extracts showed changes in abundance of specific dechlorinators as well as their functional genes. Independently of the KB-1 added, the microbial dechlorinator abundance increased to the same level in all treatments. In the non-bioaugmented microcosms the reductive dehalogenase gene bvcA increased in abundance, but when KB-1 was added the related vcrA gene increased while bvcA genes did not increase. Modeling showed higher vinyl-chloride dechlorination rates and shorter time for complete dechlorination to ethene with higher initial concentration of KB-1 culture, while cis-dichloroethene dechlorination rates were not affected by KB-1 concentrations. This study provides high resolution abundance profiles of Dehalococcoides spp. (DHC) and functional genes, highlights the ecological behavior of KB-1 in clay till, and reinforces the importance of using multiple functional genes as biomarkers for reductive dechlorination.
authors.affiliation: The Geological Survey of Denmark and Greenland (GEUS), Øster Voldgade 10, DK-1350 Copenhagen, Denmark; Technical University of Denmark (DTU), Novo Nordisk Foundation Center for Biosustainability, Fremtidsvej 3, DK-2970 Hørsholm, Denmark; The Technical University of Denmark (DTU), Center for Biological Sequence Analysis, Kemitorvet - Building 208, DK-2800 Kgs. Lyngby, Denmark.
authors.forename: Jacob
authors.initials: J
authors.lastname: Bælum
authors.affiliation: The Technical University of Denmark (DTU), Department of Environmental Engineering, Miljøvej - Building 113, DK-2800 Kgs. Lyngby, Denmark.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Scheutz
authors.affiliation: The Technical University of Denmark (DTU), Department of Environmental Engineering, Miljøvej - Building 113, DK-2800 Kgs. Lyngby, Denmark.
authors.forename: Julie C
authors.initials: JC
authors.lastname: Chambon
authors.affiliation: Capital Region of Denmark, Kongens Vænge 2, DK-3400 Hillerød, Denmark.
authors.forename: Christine Mosegaard
authors.initials: CM
authors.lastname: Jensen
authors.affiliation: The Geological Survey of Denmark and Greenland (GEUS), Øster Voldgade 10, DK-1350 Copenhagen, Denmark.
authors.forename: Rikke P
authors.initials: RP
authors.lastname: Brochmann
authors.affiliation: SiREM, 130 Research Lane Suite 2, Guelph, ON N1G 5G3, Canada.
authors.forename: Philip
authors.initials: P
authors.lastname: Dennis
authors.affiliation: The Geological Survey of Denmark and Greenland (GEUS), Øster Voldgade 10, DK-1350 Copenhagen, Denmark.
authors.forename: Troels
authors.initials: T
authors.lastname: Laier
authors.affiliation: The Technical University of Denmark (DTU), Department of Environmental Engineering, Miljøvej - Building 113, DK-2800 Kgs. Lyngby, Denmark.
authors.forename: Mette M
authors.initials: MM
authors.lastname: Broholm
authors.affiliation: The Technical University of Denmark (DTU), Department of Environmental Engineering, Miljøvej - Building 113, DK-2800 Kgs. Lyngby, Denmark.
authors.forename: Poul L
authors.initials: PL
authors.lastname: Bjerg
authors.affiliation: The Technical University of Denmark (DTU), Department of Environmental Engineering, Miljøvej - Building 113, DK-2800 Kgs. Lyngby, Denmark.
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Binning
authors.affiliation: The Geological Survey of Denmark and Greenland (GEUS), Øster Voldgade 10, DK-1350 Copenhagen, Denmark; University of Copenhagen, Thorvaldsensvej 40, DK-1958 Frederiksberg C, Denmark; Center for Permafrost, Department of Geosciences and Natural Resource Management, University of Copenhagen, Øster Voldgade 10, DK-1350 Copenhagen, Denmark. Electronic address: csj@geus.dk.
authors.forename: Carsten S
authors.initials: CS
authors.lastname: Jacobsen
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry*; D001673:Biodegradation, Environmental; D000077215:Clay; D004269:DNA, Bacterial; D005030:Ethylenes / Q000032:analysis / Q000378:metabolism; D054879:Halogenation; D007700:Kinetics; D008956:Models, Chemical; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*; D014752:Vinyl Chloride / Q000032:analysis / Q000378:metabolism*
chemical_list: D000538:Aluminum Silicates; D004269:DNA, Bacterial; D005030:Ethylenes; D012989:Soil Pollutants; C036216:ethylene; D000077215:Clay; D014752:Vinyl Chloride
keywords: Bioaugmentation; Biostimulation; Dechlorination; Dehalococcoides sp; KB-1; Modeling; qPCR