title: [Simultaneous determination of ephedrine and N-methylephedrine in urine by solid phase extraction-ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry].
abstract: A rapid and sensitive method has been developed for the simultaneous determination of ephedrine and N-methylephedrine in urine samples by solid phase extraction-ultra performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (SPE-UPLC-ESI MS/MS). The samples were extracted with Oasis MCX solid phase extraction cartridges and measured in the modes of electrospray positive ionization (ESI +) and multiple reaction monitoring (MRM). Good linearities were observed in the range of 0.025 0 - 2.50 microg/L with correlation coefficient over 0.999 0 for both analytes. The recoveries were above 80% with RSDs less than 5.0%. The limits of detection were 0.01 microg/L. The method proves to be rapid and sensitive for the trace determination of ephedrine and N-methylephedrine in urine samples.
authors.affiliation: School of Pharmacology, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Air Force General Hospital, Beijing 100142, China.
authors.forename: Fucheng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Institute of Forensic Science of Supreme People's Procuratorate, Beijing 100040, China.
authors.forename: Zhaohong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: School of Pharmacology, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: School of Pharmacology, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Xu
authors.affiliation: Interpol Corps of Yunnan Public Security Bureau, Kunming 650000, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004809:Ephedrine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: C041707:N-methylephedrine; D004809:Ephedrine