title: MicroRNA-224 inhibits progression of human prostate cancer by downregulating TRIB1.
abstract: Our previous microarray data showed that microRNA-224 (miR-224) was downregulated in human prostate cancer (PCa) tissues compared with adjacent benign tissues. However, the underlying mechanisms by which miR-224 is involved in PCa remain unclear. In this study, we identified TRIB1 as a target gene of miR-224. Forced expression of miR-224 suppressed PCa cell proliferation, invasion and migration, and promoted cell apoptosis by downregulating TRIB1. Moreover, the expression level of miR-224 in PCa tissues was negatively correlated with that of TRIB1. miR-224 downregulation was frequently found in PCa tissues with metastasis, higher PSA level and clinical stage, whereas TRIB1 upregulation was significantly associated with metastasis. Both miR-224 downregulation and TRIB1 upregulation were significantly associated with poor biochemical recurrence-free survival of patients with PCa. In conclusion, these findings reveal that the aberrant expression of miR-224 and TRIB1 may promote PCa progression and have potentials to serve as novel biomarkers for PCa prognosis.
authors.affiliation: Guangdong Provincial Institute of Nephrology, Southern Medical University, Guangzhou, China; Department of Urology Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Zhuo-Yuan
authors.initials: ZY
authors.lastname: Lin
authors.forename: Ya-Qiang
authors.initials: YQ
authors.lastname: Huang
authors.forename: Yan-Qiong
authors.initials: YQ
authors.lastname: Zhang
authors.forename: Zhao-Dong
authors.initials: ZD
authors.lastname: Han
authors.forename: Hui-Chan
authors.initials: HC
authors.lastname: He
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Ling
authors.forename: Xin
authors.initials: X
authors.lastname: Fu
authors.forename: Qi-Shan
authors.initials: QS
authors.lastname: Dai
authors.forename: Chao
authors.initials: C
authors.lastname: Cai
authors.forename: Jia-Hong
authors.initials: JH
authors.lastname: Chen
authors.forename: Yu-Xiang
authors.initials: YX
authors.lastname: Liang
authors.forename: Fu-Neng
authors.initials: FN
authors.lastname: Jiang
authors.forename: Wei-De
authors.initials: WD
authors.lastname: Zhong
authors.forename: Fen
authors.initials: F
authors.lastname: Wang
authors.forename: Chin-Lee
authors.initials: CL
authors.lastname: Wu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D002465:Cell Movement; D049109:Cell Proliferation; D018450:Disease Progression; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D011467:Prostate / Q000378:metabolism* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate; D046888:Tissue Array Analysis; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D047908:Intracellular Signaling Peptides and Proteins; C546140:MIRN224 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; C493170:TRIB1 protein, human; D017346:Protein Serine-Threonine Kinases
keywords: TRIB1; biochemical recurrence-free survival; clinicopathological feature; microRNA-224; prostate cancer