title: In vitro cytotoxicity of the ternary PAMAM G3-pyridoxal-biotin bioconjugate.
abstract: A third-generation polyamidoamine dendrimer (PAMAM G3) was used as a macromolecular carrier for pyridoxal and biotin. The binary covalent bioconjugate of G3, with nine molecules of biotin per one molecule of G3 (G3(9B)), and the ternary covalent bioconjugate of G3, with nine biotin and ten pyridoxal molecules (G3(9B10P)), were synthesized. The biotin and pyridoxal residues of the bioconjugate were available for carboxylase and transaminase enzymes, as demonstrated in the conversion of pyruvate to oxaloacetate and alanine to pyruvate, respectively, by in vitro monitoring of the reactions, using (1)H nuclear magnetic resonance spectroscopy. The toxicity of the ternary bioconjugate (BC-PAMAM) was studied in vitro on BJ human normal skin fibroblasts and human squamous cell carcinoma (SCC-15) cell cultures in comparison with PAMAM G3, using three cytotoxicity assays (XTT, neutral red, and crystal violet) and an estimation of apoptosis by confocal microscopy detection. The tests have shown that BC-PAMAM has significantly lower cytotoxicity compared with PAMAM. Nonconjugated PAMAM was not cytotoxic at concentrations up to 5 μM (NR) and 10 μM (XTT), and BC-PAMAM was not cytotoxic up to 50 μM (both assays) for both cell lines. It has been also found that normal fibroblasts were more sensitive than SCC to both PAMAM and BC-PAMAM. The effect of PAMAM and BC-PAMAM on the initiation of apoptosis (PAMAM in fibroblasts at 5 μM and BC-PAMAM at 10 μM in both cell lines) corresponded with cytotoxicity assays for both cell lines. We concluded that normal fibroblasts are more sensitive to the cytotoxic effects of the PAMAM G3 dendrimer and that modification of its surface cationic groups by substitution with biologically active molecules significantly decreases that effect, confirming that PAMAM G3 is a useful candidate as a carrier for active biocompound delivery.
authors.affiliation: Cosmetology Department, University of Information Technology and Management in Rzeszów, Rzeszów, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Uram
authors.affiliation: Cosmetology Department, University of Information Technology and Management in Rzeszów, Rzeszów, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Szuster
authors.affiliation: Cosmetology Department, University of Information Technology and Management in Rzeszów, Rzeszów, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Gargasz
authors.affiliation: Cosmetology Department, University of Information Technology and Management in Rzeszów, Rzeszów, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Filipowicz
authors.affiliation: Cosmetology Department, University of Information Technology and Management in Rzeszów, Rzeszów, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Wałajtys-Rode
authors.affiliation: Cosmetology Department, University of Information Technology and Management in Rzeszów, Rzeszów, Poland.
authors.forename: Stanisław
authors.initials: S
authors.lastname: Wołowiec
mesh_terms: D001710:Biotin / Q000737:chemistry / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects*; D002460:Cell Line; D045744:Cell Line, Tumor; D050091:Dendrimers / Q000737:chemistry / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D058726:Nanoconjugates / Q000737:chemistry*; D011730:Pyridoxal / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D050091:Dendrimers; D058726:Nanoconjugates; C104700:PAMAM Starburst; D011730:Pyridoxal; D001710:Biotin
keywords: apoptosis; biotin; enzymatic carboxylation; enzymatic transamination; normal fibroblasts; pyridoxal; squamous carcinoma cells; ternary bioconjugate