title: [Effects of rice straw returning on the community structure and diversity of nitrogen-fixing gene (nifH) in paddy soil].
abstract: Taking a long-term fertilization experiment in Taoyuan Agro-ecosystem Research Station under Chinese Academy of Sciences as the platform, and selecting four treatments (no fertilization, CK; rice straw returning, C; nitrogen, phosphorus and potassium fertilization, NPK; and NPK+C) as the objects, soil samples were collected at the tillering, booting and maturing stages of rice, and the abundance, composition and diversity of nifH-containing bacterial community were measured by real-time quantitative PCR and terminal restriction fragment length polymorphism (T-RFLP), aimed to understand the effects of rice straw returning on the nifH-containing bacterial community in paddy soil. Compared with CK, treatments NPK+C and NPK increased the abundance of nifH-containing microorganisms significantly (except at tillering stage), and NPK+C had the highest abundance of nifH-containing microorganisms. Under the effects of long-term fertilization, the composition of nifH gene community in CK differed obviously from that in the other three treatments. The nifH composition had definite difference between C and NPK, but less difference between NPK and NPK+C. Long-term fertilization did not induce significant changes in nifH diversity. Therefore, long-term rice straw returning not only induced the changes of nifH gene community composition, but also resulted in a significant increase in the abundance of nifH-containing community, and hence, the increase of soil nitrogen fixing capacity.
authors.affiliation: Key Laboratory of Agro-ecology Process in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China. zhangmiao0110@163.com
authors.forename: Miao-miao
authors.initials: MM
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Agro-ecology Process in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Agro-ecology Process in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Sheng
authors.affiliation: Key Laboratory of Agro-ecology Process in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.
authors.forename: Hong-ling
authors.initials: HL
authors.lastname: Qin
authors.affiliation: Key Laboratory of Agro-ecology Process in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.
authors.forename: Yan-zheng
authors.initials: YZ
authors.lastname: Wu
authors.affiliation: Key Laboratory of Agro-ecology Process in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.
authors.forename: Wen-xue
authors.initials: WX
authors.lastname: Wei
mesh_terms: D000383:Agriculture / Q000379:methods; D044822:Biodiversity; D005308:Fertilizers; D005798:Genes, Bacterial; D009586:Nitrogen Fixation; D012275:Oryza / Q000254:growth & development*; D010088:Oxidoreductases / Q000235:genetics*; D018547:Plant Stems; D012037:Refuse Disposal / Q000379:methods; D012988:Soil Microbiology
chemical_list: D005308:Fertilizers; D010088:Oxidoreductases; C029130:nitrogenase reductase