title: Molecular cell biology of male meiotic chromosomes and isolation of male meiocytes in Arabidopsis thaliana.
abstract: Plants typically produce numerous flowers whose meiotic chromosomes are relatively easy to observe, making them excellent structures for studying the cellular processes underlying meiosis. In recent years, breakthroughs in light and electron microscopic technologies for small chromosomes, combined with molecular genetic methods, have resulted in major advances in the understanding of meiosis in the model plant Arabidopsis thaliana. In this chapter, we summarize protocols for basic cytology, fluorescence in situ hybridization, immunofluorescence, electron microscopy, and isolation of male meiocytes for the analysis of Arabidopsis meiosis.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, China.
authors.forename: Yingxiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Cheng
authors.forename: Pingli
authors.initials: P
authors.lastname: Lu
authors.forename: Ljudmilla
authors.initials: L
authors.lastname: Timofejeva
authors.forename: Hong
authors.initials: H
authors.lastname: Ma
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000648:ultrastructure; D002469:Cell Separation / Q000379:methods*; D032461:Chromosomes, Plant / Q000378:metabolism*; D017404:In Situ Hybridization, Fluorescence; D007211:Indoles / Q000378:metabolism; D008540:Meiosis; D046529:Microscopy, Electron, Transmission; D055616:Microtechnology; D013573:Synaptonemal Complex / Q000648:ultrastructure
chemical_list: D007211:Indoles; C007293:DAPI