title: Interleukin-32δ interacts with IL-32β and inhibits IL-32β-mediated IL-10 production.
abstract: There is growing evidence for multifunctional properties of IL-32. We previously demonstrated that IL-32β upregulates IL-10 production through the association with PKCδ. In this study, we examined the effects of other IL-32 isoforms on IL-10 production. We found that IL-32δ decreased IL-10 production and investigated the inhibitory mechanism of IL-32δ. We showed that IL-32δ suppressed IL-32β binding to PKCδ by interacting with IL-32β. The inhibitory effect of IL-32δ on IL-32β association with PKCδ was further verified by immuno-fluorescence staining. The co-localization of IL-32β and PKCδ around the nuclear membrane was disrupted by IL-32δ. Our data therefore indicate that IL-32δ plays an inhibitory role against IL-32β function, which also suggests that IL-32 may be regulated by its own isoform.
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Kang
authors.forename: Yun Sun
authors.initials: YS
authors.lastname: Park
authors.forename: Dong Hun
authors.initials: DH
authors.lastname: Lee
authors.forename: Man Sub
authors.initials: MS
authors.lastname: Kim
authors.forename: Yesol
authors.initials: Y
authors.lastname: Bak
authors.forename: Su Ho
authors.initials: SH
authors.lastname: Park
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Ham
authors.forename: Young
authors.initials: Y
authors.lastname: Yang
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
authors.forename: Do-Young
authors.initials: DY
authors.lastname: Yoon
mesh_terms: D015536:Down-Regulation; D057809:HEK293 Cells; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D009685:Nuclear Envelope / Q000378:metabolism; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D051745:Protein Kinase C-delta / Q000378:metabolism; D020298:U937 Cells
chemical_list: C496971:IL32 protein, human; D007378:Interleukins; D020033:Protein Isoforms; D016753:Interleukin-10; D051745:Protein Kinase C-delta