title: Separation and identification of Musa acuminate Colla (banana) leaf proteins by two-dimensional gel electrophoresis and mass spectrometry.
abstract: To establish a proteomic reference map of Musa acuminate Colla (banana) leaf, we separated and identified leaf proteins using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MS). Tryptic digests of 44 spots were subjected to peptide mass fingerprinting (PMF) by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS. Three spots that were not identified by MALDI-TOF MS analysis were identified by searching against the NCBInr, SwissProt, and expressed sequence tag (EST) databases. We identified 41 unique proteins. The majority of the identified leaf proteins were found to be involved in energy metabolism. The results indicate that 2D-PAGE is a sensitive and powerful technique for the separation and identification of Musa leaf proteins. A summary of the identified proteins and their putative functions is discussed.
authors.affiliation: Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Hainan, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Lu
authors.forename: Y X
authors.initials: YX
authors.lastname: Qi
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: H Q
authors.initials: HQ
authors.lastname: Zhang
authors.forename: J J
authors.initials: JJ
authors.lastname: Pu
authors.forename: Y X
authors.initials: YX
authors.lastname: Xie
mesh_terms: D000595:Amino Acid Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D020224:Expressed Sequence Tags; D028521:Musa / Q000166:cytology*; D010449:Peptide Mapping; D018515:Plant Leaves / Q000166:cytology*; D010940:Plant Proteins / Q000032:analysis* / Q000145:classification / Q000302:isolation & purification; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010940:Plant Proteins; D020543:Proteome