title: Hsa-miR-15a and Hsa-miR-16-1 expression is not related to proliferation centers abundance and other prognostic factors in chronic lymphocytic leukemia.
abstract: Chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL) is the commonest leukemia in adults. Here, we aimed to evaluate hsa-miR-15a/hsa-miR-16-1 expression in CLL tissues by qPCR and correlate it with the other clinicopathological features and clinical outcome. 40 formalin-fixed paraffin-embedded (FFPE) lymph node samples obtained from CLL/SLL patients were classified into two categories, "PCs rich" and "typical." We found a significant common expression level of 4 miRNAs; however, we did not find any significant relationship between PCs presence and miRNAs expression. Moreover, neither the presence of 13q deletion nor the percentage of cells carrying the deletion strictly correlated with miRNAs expression levels, although a significant number of patients with 13q deletion presented hsa-miR-16-1-3p levels below the median value in normal samples (P < 0.05). Finally, although no correlation was found between the expression of each miRNA and other clinicopathological features (Ki67, CD38, ZAP70, and IGVH@ hypermutations), the OS curves showed a positive trend in patients with miRNAs downregulation, though not statistically significant. In conclusion, we showed for the first time that all miRNAs can be successfully studied in FFPE CLL tissues and that del13q and PCs richness do not strictly correspond to miRNAs downregulation; therefore, a specific evaluation may be envisaged at least in patients enrolled in clinical trials.
authors.affiliation: Department of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School, Unit of Hematopathology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy.
authors.forename: Maura
authors.initials: M
authors.lastname: Rossi
authors.affiliation: Department of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School, Unit of Hematopathology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Fuligni
authors.affiliation: Department of Biomedical Sciences, Hematology Section, S. Anna Hospital, University of Ferrara, Via Aldo Moro 8, Cona, 44124 Ferrara, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Ciccone
authors.affiliation: Department of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School, Unit of Hematopathology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Agostinelli
authors.affiliation: Department of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School, Unit of Hematopathology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Righi
authors.affiliation: Department of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School, Unit of Hematopathology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Luciani
authors.affiliation: Department of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School, Unit of Hematopathology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy.
authors.forename: Maria Antonella
authors.initials: MA
authors.lastname: Laginestra
authors.affiliation: Department of Biomedical Sciences, Hematology Section, S. Anna Hospital, University of Ferrara, Via Aldo Moro 8, Cona, 44124 Ferrara, Italy.
authors.forename: Gian Matteo
authors.initials: GM
authors.lastname: Rigolin
authors.affiliation: Department of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School, Unit of Hematopathology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy.
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Sapienza
authors.affiliation: Department of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School, Unit of Hematopathology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Gazzola
authors.affiliation: Department of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School, Unit of Hematopathology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Mannu
authors.affiliation: Department of Biomedical Sciences, Hematology Section, S. Anna Hospital, University of Ferrara, Via Aldo Moro 8, Cona, 44124 Ferrara, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Cuneo
authors.affiliation: Department of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School, Unit of Hematopathology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Pileri
authors.affiliation: Department of Experimental, Diagnostic, and Specialty Medicine, Bologna University Medical School, Unit of Hematopathology, S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy.
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Piccaluga
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D049109:Cell Proliferation; D002872:Chromosome Deletion; D025063:Chromosome Disorders / Q000235:genetics*; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics; D005260:Female; D015973:Gene Expression Regulation, Leukemic / Q000235:genetics; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000473:pathology; D008198:Lymph Nodes / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D016612:Paraffin Embedding
chemical_list: C505361:MIRN15 microRNA, human; C505362:MIRN16 microRNA, human; D035683:MicroRNAs