title: Identification of histone 3 variant 2 interacting factors.
abstract: The epigenome is defined as a type of information that can be transmitted independently of the DNA sequence, at the chromatin level, through post-translational modifications present on histone tails. Recent advances in the identification of histone 3 variants suggest a new model of information transmission through deposition of specific histone variants. To date, several non-centromeric histone 3 variants have been identified in mammals. Despite protein sequence similarity, specific deposition complexes have been characterized for both histone 3.1 (H3.1) and histone 3.3 (H3.3), whereas no deposition complex for histone 3.2 (H3.2) has been identified to date. Here, we identified human H3.2 partners by immunopurification of nuclear H3.2 complexes followed by mass spectrometry analysis. Further biochemical analyses highlighted two major complexes associated with H3.2, one containing chromatin associated factor-1 subunits and the other consisting of a subcomplex of mini chromosome maintenance helicases, together with Asf1. The purified complexes could associate with a DNA template in vitro.
authors.affiliation: Laboratoire de Régulation des Gènes, Institut de Génétique Humaine, CNRS UPR1142, Montpellier 34396, France and Laboratoire de Virologie Moléculaire, Institut de Génétique Humaine, CNRS UPR1142, Montpellier 34396, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Latreille
authors.forename: Lisa
authors.initials: L
authors.lastname: Bluy
authors.forename: Monsef
authors.initials: M
authors.lastname: Benkirane
authors.forename: Rosemary E
authors.initials: RE
authors.lastname: Kiernan
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism*; D006801:Humans; D064110:Minichromosome Maintenance Proteins / Q000378:metabolism; D018832:Molecular Chaperones; D016196:S Phase
chemical_list: C491641:ASF1A protein, human; C491640:ASF1B protein, human; D018797:Cell Cycle Proteins; D002843:Chromatin; D006657:Histones; D018832:Molecular Chaperones; D064110:Minichromosome Maintenance Proteins