title: C & N isotope analysis of diclofenac to distinguish oxidative and reductive transformation and to track commercial products.
abstract: Although diclofenac is frequently found in aquatic systems, its degradability in the environment remains imperfectly understood. On the one hand, evidence from concentration analysis alone is inconclusive if an unknown hydrology impedes a distinction between degradation and dilution. On the other hand, not all transformation products may be detectable. As a new approach, we therefore developed GC-IRMS (gas chromatography-isotope-ratio mass-spectrometry) analysis for carbon and nitrogen isotope measurements of diclofenac. The method uses a derivatization step that can be conducted either online or offline, for optimized throughput or sensitivity, respectively. In combination with on-column injection, the latter method enables determination of diclofenac isotope ratios down to the sub-μgL(-1) range in environmental samples. Degradation in an aerobic sediment-water system showed strong nitrogen isotope fractionation (εN = -7.1‰), whereas reductive diclofenac dechlorination was associated with significant carbon isotope fractionation (εC = -2.0‰). Hence dual element isotope analysis bears potential not only to detect diclofenac degradation, but even to distinguish both transformation pathways in the environment. In an explorative survey, analysis of commercial diclofenac products showed significant differences in carbon and nitrogen isotope ratios, demonstrating a further potential to track, and potentially even to authenticate, commercial production batches.
authors.affiliation: Helmholtz Zentrum Muenchen, German Research Center, Institute of Groundwater Ecology , Ingolstädter Landstrasse 1, Neuherberg D-85764, Germany.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Maier
authors.forename: Simon
authors.initials: S
authors.lastname: De Corte
authors.forename: Sebastian
authors.initials: S
authors.lastname: Nitsche
authors.forename: Thomas
authors.initials: T
authors.lastname: Spaett
authors.forename: Nico
authors.initials: N
authors.lastname: Boon
authors.forename: Martin
authors.initials: M
authors.lastname: Elsner
mesh_terms: D000332:Aerobiosis; D001673:Biodegradation, Environmental; D001711:Biotransformation; D002247:Carbon Isotopes; D005591:Chemical Fractionation; D004008:Diclofenac / Q000032:analysis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D019015:Geologic Sediments / Q000737:chemistry; D054879:Halogenation; D007700:Kinetics; D057230:Limit of Detection; D009587:Nitrogen Isotopes; D010084:Oxidation-Reduction; D012015:Reference Standards; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D014874:Water Pollutants, Chemical; D004008:Diclofenac; D012822:Silicon Dioxide