title: Validated LC-MS/MS method for the simultaneous determination of hyperoside and 2''-O-galloylhyperin in rat plasma: application to a pharmacokinetic study in rats.
abstract: An LC-MS/MS method was developed for the first time to simultaneously determine hyperoside and 2''-O-galloylhyperin, two major components in Pyrola calliantha extract, in rat plasma. Following extraction by one-step protein precipitation with methanol, the analytes were separated on a Venusil MP-C18 column within 2 min, using methanol-water-formic acid (50:50:0.1, v/v/v) as the mobile phase at a flow rate of 0.4 mL/min. Detection was performed on electrospray negative ionization mass spectrometry by multiple-reaction monitoring of the transitions of 2''-O-galloylhyperin at m/z 615.1 → 301.0, of hyperoside at m/z 463.1 → 300.1, and of internal standard at m/z 415.1 → 295.1. The limits of quantification were 2 ng/mL for both hyperoside and 2''-O-galloylhyperin. The precisions were <13.1%, and the accuracies were between -9.1 and 5.5% for both compounds. The method was successfully applied in pharmacokinetic studies following intravenous administration of the total flavonoids of P. calliantha extract in rats.
authors.affiliation: Department of Cardiology, The Fourth Affiliated Hospital of China Medical University, Shenyang, 110032, Liaoning, China.
authors.forename: Donghui
authors.initials: D
authors.lastname: Zhou
authors.forename: Yuanzhe
authors.initials: Y
authors.lastname: Jin
authors.forename: Fengchen
authors.initials: F
authors.lastname: Yao
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Duan
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D011794:Quercetin / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C087939:2''-galloylhyperin; D005707:Gallic Acid; C021304:hyperoside; D011794:Quercetin
keywords: 2′′-O-galloylhyperin; LC-MS/MS; hyperoside; pharmacokinetic study; simultaneous determination