title: A CssA, CssB and LTB chimeric protein induces protection against Enterotoxigenic Escherichia coli.
abstract: OBJECTIVE
  Enterotoxigenic Escherichia coli (ETEC), a major cause of diarrhea in children under 5, is an important agent for traveler's diarrhea. Heat-labile enterotoxin (LT) and colonization factors (CFs) are two main virulence mechanisms in ETEC. CS6 is one of the most prevalent CFs consisting of two structural subunits viz., CssA, CssB, necessary for attachment to the intestinal cells.
  
  
  METHODS
  In the present research, a chimeric trivalent protein composed of CssB, CssA and LTB was constructed. The chimeric gene was synthesized with codon bias of E. coli for enhanced expression of the protein. Recombinant proteins were expressed and purified. Mice were immunized with the recombinant protein. The antibody titer and specificity of the immune sera were analyzed by ELISA and Western blotting. Efficiency of the immune sera against ETEC was evaluated.
  
  
  RESULTS
  Antibody induction was followed by immunization of mice with the chimeric protein. Pretreatment of the ETEC cells with immunized animal antisera remarkably decreased their adhesion to Caco-2 cells.
  
  
  CONCLUSIONS
  The results indicate efficacy of the recombinant chimeric protein as an effective immunogen, which induces strong humoral response as well as protection against ETEC adherence and toxicity.
authors.affiliation: Department of Biology, Shahed University, Tehran, Iran.
authors.forename: Samane
authors.initials: S
authors.lastname: Bagheri
authors.affiliation: Department of Biology, Shahed University, Tehran, Iran. Electronic address: slmousavi@shahed.ac.ir.
authors.forename: Seyed Latif
authors.initials: SL
authors.lastname: Mousavi Gargari
authors.affiliation: Department of Biology, Shahed University, Tehran, Iran.
authors.forename: Iraj
authors.initials: I
authors.lastname: Rasooli
authors.affiliation: Department of Biology, Imam Hussein University, Tehran, Iran.
authors.forename: Shahram
authors.initials: S
authors.lastname: Nazarian
authors.affiliation: Department of Biology, Shahed University, Tehran, Iran.
authors.forename: Masoome
authors.initials: M
authors.lastname: Alerasol
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics*; D015153:Blotting, Western; D020740:Chimerin Proteins / Q000737:chemistry / Q000276:immunology*; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics* / Q000276:immunology; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000942:Antigens, Bacterial; C098406:CS6 antigen, E coli; D020740:Chimerin Proteins; D029968:Escherichia coli Proteins
keywords: CS6; Chimeric protein; Enterotoxigenic Escherichia coli; Enterotoxin