title: Validity and applicability of membrane model systems for studying interactions of peripheral membrane proteins with lipids.
abstract: The cell membrane serves, at the same time, both as a barrier that segregates as well as a functional layer that facilitates selective communication. It is characterized as much by the complexity of its components as by the myriad of signaling process that it supports. And, herein lays the problems in its study and understanding of its behavior - it has a complex and dynamic nature that is further entangled by the fact that many events are both temporal and transient in their nature. Model membrane systems that bypass cellular complexity and compositional diversity have tremendously accelerated our understanding of the mechanisms and biological consequences of lipid-lipid and protein-lipid interactions. Concurrently, in some cases, the validity and applicability of model membrane systems are tarnished by inherent methodical limitations as well as undefined quality criteria. In this review we introduce membrane model systems widely used to study protein-lipid interactions in the context of key parameters of the membrane that govern lipid availability for peripheral membrane proteins. This article is part of a Special Issue entitled Tools to study lipid functions.
authors.affiliation: Laboratory of Membrane Biochemistry, Paul Langerhans Institute Dresden, Faculty of Medicine Carl Gustav Carus at the TU Dresden, Fetscherstrasse 74, 01307 Dresden, Germany; German Center for Diabetes Research (DZD), Germany. Electronic address: czogalla@plid.de.
authors.forename: Aleksander
authors.initials: A
authors.lastname: Czogalla
authors.affiliation: Laboratory of Membrane Biochemistry, Paul Langerhans Institute Dresden, Faculty of Medicine Carl Gustav Carus at the TU Dresden, Fetscherstrasse 74, 01307 Dresden, Germany; German Center for Diabetes Research (DZD), Germany.
authors.forename: Michał
authors.initials: M
authors.lastname: Grzybek
authors.affiliation: Laboratory of Membrane Biochemistry, Paul Langerhans Institute Dresden, Faculty of Medicine Carl Gustav Carus at the TU Dresden, Fetscherstrasse 74, 01307 Dresden, Germany; German Center for Diabetes Research (DZD), Germany.
authors.forename: Walis
authors.initials: W
authors.lastname: Jones
authors.affiliation: Laboratory of Membrane Biochemistry, Paul Langerhans Institute Dresden, Faculty of Medicine Carl Gustav Carus at the TU Dresden, Fetscherstrasse 74, 01307 Dresden, Germany; German Center for Diabetes Research (DZD), Germany. Electronic address: coskun@plid.de.
authors.forename: Unal
authors.initials: U
authors.lastname: Coskun
mesh_terms: D008051:Lipid Bilayers; D008081:Liposomes; D008563:Membrane Lipids / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008954:Models, Biological; D011485:Protein Binding
chemical_list: D008051:Lipid Bilayers; D008081:Liposomes; D008563:Membrane Lipids; D008565:Membrane Proteins
keywords: Lipid identity; Lipid presentation; Membrane; Model membrane system; Phospholipid; Protein–lipid interaction