title: Ultrasound assisted enzymatic pre-treatment of high fat content dairy wastewater.
abstract: This paper illustrates the application of ultrasound in a dairy waste water treatment for the removal of fat using enzyme as a catalyst. Lipase Z was used to perform the enzymatic pre-hydrolysis of a synthetic dairy wastewater containing around 2000 mg/L of fat content coupled with ultrasound irradiation. Different process parameters like effect of enzyme loading, temperature, ultrasound power, frequency, duty cycle and speed of agitation are optimized. The maximum hydrolysis of 78% is achieved at 0.2% enzyme loading (w/v), 30°C temperature, 165 W of ultrasonication power at 25 kHz and 66% duty cycle. It was observed that the enzymatic pre-hydrolysis under the influence of ultrasound drastically reduces the reaction time from 24h to 40 min as compared to conventional stirring with improved yield.
authors.affiliation: Department of Chemical Engineering, Institute of Chemical Technology, Matunga (E), Mumbai 400019, India.
authors.forename: Tejal V
authors.initials: TV
authors.lastname: Adulkar
authors.affiliation: Department of Chemical Engineering, Institute of Chemical Technology, Matunga (E), Mumbai 400019, India. Electronic address: vk.rathod@ictmumbai.edu.in.
authors.forename: Virendra K
authors.initials: VK
authors.lastname: Rathod
mesh_terms: D055162:Biocatalysis; D002175:Candida / Q000201:enzymology; D003612:Dairying; D005223:Fats / Q000737:chemistry*; D007220:Industrial Waste; D008049:Lipase / Q000378:metabolism*; D012965:Sodium Chloride / Q000737:chemistry; D013010:Sonication / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D014465:Ultrasonics / Q000379:methods*; D062065:Wastewater / Q000737:chemistry*
chemical_list: D005223:Fats; D007220:Industrial Waste; D008049:Lipase; D012965:Sodium Chloride; D062065:Wastewater
keywords: Anaerobic digestion; Dairy wastewater; Lipase Z; Oils and grease; Ultrasonic irradiation