title: An excimer-based, turn-on fluorescent sensor for the selective detection of diphosphorylated proteins in aqueous solution and polyacrylamide gels.
abstract: Protein phosphorylation is a ubiquitous post-translational modification, which often acts as a switch to proteins' activation and is frequently perturbed in diseases. Although many general phospho-protein detection tools are available, none of them offers information about the relative spatial arrangement of phosphorylated residues. Specifically, proximally phosphorylated residues are hallmarks of certain activated disease-relevant proteins. We herein report the first turn-on fluorescent sensor for the selective detection of proximally phosphorylated protein sites, suitable for application in both aqueous solutions and polyacrylamide gels.
authors.affiliation: Department of Chemistry and Department of Chemical and Physical Sciences, University of Toronto, Mississauga , 3359 Mississauga Road North, Mississauga, Ontario, Canada L5L1C6.
authors.forename: Dziyana
authors.initials: D
authors.lastname: Kraskouskaya
authors.forename: Matthew
authors.initials: M
authors.lastname: Bancerz
authors.forename: Harjeet S
authors.initials: HS
authors.lastname: Soor
authors.forename: Jordan E
authors.initials: JE
authors.lastname: Gardiner
authors.forename: Patrick T
authors.initials: PT
authors.lastname: Gunning
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005782:Gels / Q000737:chemistry*; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry*; D010766:Phosphorylation; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D012996:Solutions; D014867:Water / Q000737:chemistry; D015032:Zinc / Q000737:chemistry; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D005456:Fluorescent Dyes; D005782:Gels; D009942:Organometallic Compounds; D011506:Proteins; D012996:Solutions; D014867:Water; D015032:Zinc; D000099166:Polyacrylamides