title: HPLC analysis and standardization of Brahmi vati - An Ayurvedic poly-herbal formulation.
abstract: OBJECTIVE
  The aim of the present study was to standardize Brahmi vati (BV) by simultaneous quantitative estimation of Bacoside A3 and Piperine adopting HPLC-UV method. BV very important Ayurvedic polyherbo formulation used to treat epilepsy and mental disorders containing thirty eight ingredients including Bacopa monnieri L. and Piper longum L.
  
  
  METHODS
  An HPLC-UV method was developed for the standardization of BV in light of simultaneous quantitative estimation of Bacoside A3 and Piperine, the major constituents of B. monnieri L. and P. longum L. respectively. The developed method was validated on parameters including linearity, precision, accuracy and robustness.
  
  
  RESULTS
  The HPLC analysis showed significant increase in amount of Bacoside A3 and Piperine in the in-house sample of BV when compared with all three different marketed samples of the same. Results showed variations in the amount of Bacoside A3 and Piperine in different samples which indicate non-uniformity in their quality which will lead to difference in their therapeutic effects.
  
  
  CONCLUSIONS
  The outcome of the present investigation underlines the importance of standardization of Ayurvedic formulations. The developed method may be further used to standardize other samples of BV or other formulations containing Bacoside A3 and Piperine.
authors.affiliation: Department of Pharmacognosy, School of Pharmaceutical Sciences, IFTM University, Moradabad 244001, India ; Department of Pharmaceutical Sciences, Birla Institute of Technology, Ranchi 835215, India.
authors.forename: Amrita
authors.initials: A
authors.lastname: Mishra
authors.affiliation: Department of Pharmacognosy, School of Pharmaceutical Sciences, IFTM University, Moradabad 244001, India.
authors.forename: Arun K
authors.initials: AK
authors.lastname: Mishra
authors.affiliation: Department of Pharmacognosy, School of Pharmaceutical Sciences, IFTM University, Moradabad 244001, India.
authors.forename: Om Prakash
authors.initials: OP
authors.lastname: Tiwari
authors.affiliation: Department of Pharmaceutical Sciences, Birla Institute of Technology, Ranchi 835215, India.
authors.forename: Shivesh
authors.initials: S
authors.lastname: Jha
keywords: Bacoside A3; Brahmi vati; HPLC; Piperine