title: 17β-estradiol protects human eyelid-derived adipose stem cells against cytotoxicity and increases transplanted cell survival in spinal cord injury.
abstract: Stem cell transplantation represents a promising strategy for the repair of spinal cord injury (SCI). However, the low survival rate of the grafted cells is a major obstacle hindering clinical success because of ongoing secondary injury processes, which includes excitotoxicity, inflammation and oxidative stress. Previous studies have shown that 17b-estradiol (E2) protects several cell types against cytotoxicity. Thus, we examined the effects of E2 on the viability of human eyelid adipose-derived stem cells (hEASCs) in vitro with hydrogen peroxide (H₂O₂)-induced cell model and in vivo within a rat SCI model. Our results showed that E2 protected hEASCs against H₂O₂-induced cell death in vitro, and enhanced the survival of grafted hEASCs in vivo by reducing apoptosis. Additionally, E2 also enhanced the secretion of growth factors by hEASCs, thereby making the local microenvironment more conducive for tissue regeneration. Overall, E2 administration enhanced the therapeutic efficacy of hEASCs transplantation and facilitated motor function recovery after SCI. Hence, E2 administration may be an intervention of choice for enhancing survival of transplanted hEASCs after SCI.
authors.affiliation: Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou, China; Zhejiang Provincial Key Laboratory of Tissue Engineering and Regenerative Medicine, Hangzhou, China; Institute of Anatomy and Cell Biology, Medical College, Zhejiang University, Hangzhou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.forename: Ping
authors.initials: P
authors.lastname: Lu
authors.forename: Hao
authors.initials: H
authors.lastname: Ren
authors.forename: Zefeng
authors.initials: Z
authors.lastname: Zheng
authors.forename: Junfeng
authors.initials: J
authors.lastname: Ji
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.forename: Fangzhen
authors.initials: F
authors.lastname: Jiang
authors.forename: Shucai
authors.initials: S
authors.lastname: Ling
authors.forename: Boon Chin
authors.initials: BC
authors.lastname: Heng
authors.forename: Xueqing
authors.initials: X
authors.lastname: Hu
authors.forename: HongWei
authors.initials: H
authors.lastname: Ouyang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000276:immunology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology*; D005143:Eyelids / Q000166:cytology / Q000276:immunology; D006085:Graft Survival / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D013119:Spinal Cord Injuries / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D014183:Transplantation, Heterologous; D016896:Treatment Outcome
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D004958:Estradiol; D006861:Hydrogen Peroxide
keywords: 17-β-estradiol; cell transplantation; hEASCs; spinal cord injury