title: Gemcitabine-treated pancreatic cancer cell medium induces the specific CTL antitumor activity by stimulating the maturation of dendritic cells.
abstract: Gemcitabine (GEM) is a first line chemotherapeutic drug for advanced pancreatic cancer. Dendritic cell (DC) vaccine is a promising method of immunotherapy for malignant tumor. Recent research has indicated that gemcitabine can enhance the efficacy of DC vaccine, but precise mechanism is still unknown. Here, we aimed to investigate the effect of GEM on DCs. The results showed that GEM-treated pancreatic cancer cell medium stimulated maturation of DCs. When co-cultured with autologous T lymphocytes, the pulsed DCs promoted the proliferation of T cells, and exhibited specific cytotoxic T lymphocytes (CTLs) antitumor activity. Further research showed that stimulation of DC maturation may be related to the elevated level of Hsp70 induced by GEM. Our study indicates that GEM changes the immunogenicity of tumor cells, and enhances the efficacy of DC based immunotherapy for pancreatic cancer.
authors.affiliation: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Qingshan
authors.initials: Q
authors.lastname: Pei
authors.affiliation: Department of Gastroenterology, Jinan Central Hospital Affiliated to Shandong University, Jinan, China.
authors.forename: Jianmei
authors.initials: J
authors.lastname: Pan
authors.affiliation: Department of Gastroenterology, The Affiliated Drum Tower Hospital of Nanjing University, Medical School, Nanjing, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Gastroenterology, The Affiliated Drum Tower Hospital of Nanjing University, Medical School, Nanjing, China.
authors.forename: Xiwei
authors.initials: X
authors.lastname: Ding
authors.affiliation: Department of Gastroenterology, The Affiliated Drum Tower Hospital of Nanjing University, Medical School, Nanjing, China.
authors.forename: Wenjia
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, The Affiliated Drum Tower Hospital of Nanjing University, Medical School, Nanjing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Department of Gastroenterology, The Affiliated Drum Tower Hospital of Nanjing University, Medical School, Nanjing, China. Electronic address: zouxiaoping795@hotmail.com.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zou
authors.affiliation: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, China. Electronic address: xhnk@163.com.
authors.forename: Hesheng
authors.initials: H
authors.lastname: Luo
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology*; D006801:Humans; D010190:Pancreatic Neoplasms / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects* / Q000276:immunology; D000093542:Gemcitabine
chemical_list: D000964:Antimetabolites, Antineoplastic; D003470:Culture Media; D018840:HSP70 Heat-Shock Proteins; D003841:Deoxycytidine; D000093542:Gemcitabine
keywords: Chemotherapy; Dendritic cell; Gemcitabine; Immunotherapy; Pancreatic cancer