title: Fermented wheat powder induces the antioxidant and detoxifying system in primary rat hepatocytes.
abstract: Many plants exhibit antioxidant properties which may be useful in the prevention of oxidative stress reactions, such as those mediated by the formation of free radical species in different pathological situations. In recent years a number of studies have shown that whole grain products in particular have strong antioxidant activity. Primary cultures of rat hepatocytes were used to investigate whether and how a fermented powder of wheat (Lisosan G) is able to modulate antioxidant and detoxifying enzymes, and whether or not it can activate Nrf2 transcription factor or inhibit NF-kB activation. All of the antioxidant and detoxifying enzymes studied were significantly up-regulated by 0.7 mg/ml Lisosan G treatment. In particular,
  quinone oxidoreductase and heme oxygenase-1 were induced, although to different degrees, at the transcriptional, protein and/or activity levels by the treatment. As for the Nrf2 transcription factor, a partial translocation of its protein from the cytosol to the nucleus after 1 h of Lisosan G treatment was revealed by immunoblotting. Lisosan G was also observed to decrease H2O2-induced toxicity Taken together, these results show that this powder of wheat is an effective inducer of ARE/Nrf2-regulated antioxidant and detoxifying genes and has the potential to inhibit the translocation of NF-kB into the nucleus.
authors.affiliation: Istituto di Biologia e Biotecnologia Agraria, CNR, Pisa, Italy.
authors.forename: Margherita
authors.initials: M
authors.lastname: La Marca
authors.affiliation: Istituto di Fisiologia Clinica, CNR, Pisa, Italy.
authors.forename: Pascale
authors.initials: P
authors.lastname: Beffy
authors.affiliation: Istituto di Biologia e Biotecnologia Agraria, CNR, Pisa, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Pugliese
authors.affiliation: Istituto di Biologia e Biotecnologia Agraria, CNR, Pisa, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Longo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism* / Q000494:pharmacology*; D002478:Cells, Cultured; D005285:Fermentation; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000633:toxicity; D050217:Metabolic Detoxication, Phase II; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D028321:Plant Preparations; D011208:Powders; D021381:Protein Transport / Q000187:drug effects; D051381:Rats; D014908:Triticum / Q000737:chemistry*
chemical_list: D000975:Antioxidants; C522933:Lisosan G; D051267:NF-E2-Related Factor 2; D016328:NF-kappa B; C495637:Nfe2l2 protein, rat; D010936:Plant Extracts; D028321:Plant Preparations; D011208:Powders; D006861:Hydrogen Peroxide