title: Protective effect of tomatine against hydrogen peroxide-induced neurotoxicity in neuroblastoma (SH-SY5Y) cells.
abstract: OBJECTIVE
  Reactive oxygen species can induce cell apoptosis, and oxidative stress has been implicated in a variety of neurodegenerative disorders. Tomatine, which is a naturally occurring steroidal glycoalkaloid isolated from Solanum cathayanum, has shown potent anti-oxidant properties.
  
  
  METHODS
  In this study, we used the SH-SY5Y cell line as an in vitro model and investigated the protective effect of tomatine against hydrogen peroxide (H2 O2 )-induced neurotoxicity in SH-SY5Y cells.
  
  
  RESULTS
  Tomatine might inhibit the release of cellular lactate dehydrogenase, increase anti-oxidant enzyme activity and glutathione content, reverse the downregulated protein expression of the brain-derived neurotrophic factor (BDNF), inhibit expression of Bax and activations of caspase-3 and caspase-9 in H2 O2 -induced SH-SY5Y cells.
  
  
  CONCLUSIONS
  Tomatine exerted beneficially neuroprotective effect on H2 O2 -induced SH-SY5Y cells, mainly enhancing intracellular anti-oxidant enzyme activity and BDNF expression, inhibiting H2 O2 -induced oxidative stress as well as expression of Bax and activations of caspase-3 and caspase-9, alleviating H2 O2 -induced SH-SY5Y cell injury and cell death.
authors.affiliation: Hubei Key Laboratory of Natural Products Research and Development, College of Chemistry and Life Science, China Three Gorges University, Yichang, China.
authors.forename: Shao-lan
authors.initials: SL
authors.lastname: Huang
authors.forename: Hai-bo
authors.initials: HB
authors.lastname: He
authors.forename: Kun
authors.initials: K
authors.lastname: Zou
authors.forename: Cai-hong
authors.initials: CH
authors.lastname: Bai
authors.forename: Yan-hong
authors.initials: YH
authors.lastname: Xue
authors.forename: Jun-zhi
authors.initials: JZ
authors.lastname: Wang
authors.forename: Jian-feng
authors.initials: JF
authors.lastname: Chen
mesh_terms: D019208:Brain-Derived Neurotrophic Factor / Q000032:analysis; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009447:Neuroblastoma / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D014053:Tomatine / Q000494:pharmacology*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D018696:Neuroprotective Agents; D014053:Tomatine; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D053148:Caspase 3; D053453:Caspase 9
keywords: SH-SY5Y cell; apoptosis; neurotoxicity; reactive oxygen species; tomatine