title: A genetic variant in pre-miR-27a is associated with a reduced cervical cancer risk in southern Chinese women.
abstract: OBJECTIVE
  MicroRNAs (miRNAs) play critical roles in cervical carcinogenesis. Common single nucleotide polymorphisms (SNPs) in pre/pri-miRNAs may change their property through altering miRNAs expression and/or maturation. Here we aimed to investigate the influence of three common SNPs in pre/pri-miRNAs (pri-miR-26a-1 rs7372209, pre-miR-27a rs895819 and pri-miR-100 rs1834306) on individual susceptibility to cervical cancer.
  
  
  METHODS
  We genotyped these three polymorphisms in 103 cervical cancer cases and 417 cancer-free female subjects using polymerase chain reaction-ligation detection reaction (PCR-LDR) method. Unconditional logistic regression analysis was utilized to estimate the association between these polymorphisms and the risk of cervical cancer.
  
  
  RESULTS
  In a logistic regression analysis, we found that the rs895819 polymorphism in pre-miR-27a exhibited a significant effect on cervical cancer risk; T allele (OR = 0.68, 95% CI = 0.49-0.95, P = 0.025), and CT (OR = 0.33, 95% CI = 0.15-0.74, P = 0.007) or TT (OR = 0.33, 95% C I= 0.15-0.72, P = 0.006) genotype were associated with the decreased risk, compared to C and CC respectively. As we used further genotype association models, we found a similar trend of the association in additive (OR = 0.70, P = 0.041) and recessive model (OR = 0.33, P = 0.004). We did not detect any association of the other two SNPs in pri-miR-26a-1 (rs7372209) and pri-miR-100 (rs1834306) with cervical cancer risk.
  
  
  CONCLUSIONS
  Our study provides the first evidence that the miR-27a rs895819 polymorphism is associated with a decreased risk of cervical cancer in southern Chinese women.
authors.affiliation: Institute of Aging Research, Guangdong Medical College, Dongguan 523808, China; Institute of Biochemistry & Molecular Biology, Guangdong Medical College, Zhanjiang 524023, China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan 523808, China; Key Laboratory of Molecular Biology in High Cancer Incidence Coastal Chaoshan Area of Guangdong Higher Education Institutes, Medical College of Shantou University, Shantou 515041, China. Electronic address: xiongxingdong@126.com.
authors.forename: Xing-Dong
authors.initials: XD
authors.lastname: Xiong
authors.affiliation: Department of Gynecology, Guangdong Women and Children Hospital and Health Institute, Guangzhou 510010, China.
authors.forename: Xi-Ping
authors.initials: XP
authors.lastname: Luo
authors.affiliation: Institute of Aging Research, Guangdong Medical College, Dongguan 523808, China; Institute of Biochemistry & Molecular Biology, Guangdong Medical College, Zhanjiang 524023, China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan 523808, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Institute of Aging Research, Guangdong Medical College, Dongguan 523808, China; Institute of Biochemistry & Molecular Biology, Guangdong Medical College, Zhanjiang 524023, China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan 523808, China.
authors.forename: Xinguang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Molecular Biology in High Cancer Incidence Coastal Chaoshan Area of Guangdong Higher Education Institutes, Medical College of Shantou University, Shantou 515041, China; Department of Biochemistry and Molecular Biology, Medical College of Shantou University, Shantou 515041, China.
authors.forename: En-Min
authors.initials: EM
authors.lastname: Li
authors.affiliation: Department of Gynecology, Guangdong Women and Children Hospital and Health Institute, Guangzhou 510010, China.
authors.forename: Li-Qin
authors.initials: LQ
authors.lastname: Zeng
mesh_terms: D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D035683:MicroRNAs / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012322:RNA Precursors / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
chemical_list: C531388:MIRN27 microRNA, human; D035683:MicroRNAs; D012322:RNA Precursors
keywords: Cervical cancer; Risk; Single nucleotide polymorphism; miRNA