title: Anti-IL-17 antibody improves hepatic steatosis by suppressing interleukin-17-related fatty acid synthesis and metabolism.
abstract: To investigate the relationship between interleukin-17 and proteins involved in fatty acid metabolism with respect to alcoholic liver disease, male ICR mice were randomized into five groups: control, alcoholic liver disease (ALD) at 4 weeks, 8 weeks, and 12 weeks, and anti-IL-17 antibody treated ALD. A proteomic approach was adopted to investigate changes in liver proteins between control and ALD groups. The proteomic analysis was performed by two-dimensional difference gel electrophoresis. Spots of interest were subsequently subjected to nanospray ionization tandem mass spectrometry (MS/MS) for protein identification. Additionally, expression levels of selected proteins were confirmed by western blot. Transcriptional levels of some selected proteins were determined by RT-PCR. Expression levels of 95 protein spots changed significantly (ratio >1.5, P < 0.05) during the development of ALD. Sterol regulatory element-binding protein-lc (SREBP-1c), carbohydrate response element binding protein (ChREBP), enoyl-coenzyme A hydratase (ECHS1), and peroxisome proliferator-activated receptor alpha (PPAR-α) were identified by MS/MS among the proteins shown to vary the most; increased IL-17 elevated the transcription of SREBP-1c and ChREBP but suppressed ECHS1 and PPAR-α. The interleukin-17 signaling pathway is involved in ALD development; anti-IL-17 antibody improved hepatic steatosis by suppressing interleukin-17-related fatty acid metabolism.
authors.affiliation: Translational Medicine Research Center, Jiangning Hospital Affiliated to Nanjing Medical University, Nanjing 211100, China ; Key Laboratory of Living Donor Liver Transplantation of Ministry of Public Health, Nanjing 210029, China ; General Surgery, Second People's Hospital of Nantong, Nantong 226002, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Shi
authors.affiliation: Nanjing Children's Hospital Affiliated to Nanjing Medical University, Nanjing 210008, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Translational Medicine Research Center, Jiangning Hospital Affiliated to Nanjing Medical University, Nanjing 211100, China ; Key Laboratory of Living Donor Liver Transplantation of Ministry of Public Health, Nanjing 210029, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Gu
authors.affiliation: Translational Medicine Research Center, Jiangning Hospital Affiliated to Nanjing Medical University, Nanjing 211100, China.
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Translational Medicine Research Center, Jiangning Hospital Affiliated to Nanjing Medical University, Nanjing 211100, China ; Key Laboratory of Living Donor Liver Transplantation of Ministry of Public Health, Nanjing 210029, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Lu
authors.affiliation: Translational Medicine Research Center, Jiangning Hospital Affiliated to Nanjing Medical University, Nanjing 211100, China ; Key Laboratory of Living Donor Liver Transplantation of Ministry of Public Health, Nanjing 210029, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Qian
authors.affiliation: Department of General Surgery, Second Affiliated Hospital of Nanjing Medical University, Nanjing 210000, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Shen
authors.affiliation: Key Laboratory of Living Donor Liver Transplantation of Ministry of Public Health, Nanjing 210029, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Living Donor Liver Transplantation of Ministry of Public Health, Nanjing 210029, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000494:pharmacology*; D002460:Cell Line; D005227:Fatty Acids / Q000378:metabolism*; D005234:Fatty Liver / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D020381:Interleukin-17 / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D008099:Liver / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D040901:Proteomics / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal; D005227:Fatty Acids; D020381:Interleukin-17