title: Efficient function and characterization of GH10 xylanase (Xyl10g) from Gloeophyllum trabeum in lignocellulose degradation.
abstract: The xylanase gene from Gloeophyllum trabeum was cloned and expressed in Pichia pastoris GS115. Xyl10g has a molecular weight of approximately 50kDa, and exhibits maximum specific activity at 70°C and a broad range of pH 4.0-7.0. Purified recombinant Xyl10g efficiently degraded popping-pretreated corn stover and newspaper waste at 50°C and pH 4.0 after 24h, and showed synergistic effects with Cel5B (endoglucanase) and BglB (β-glucosidase) to increase reduced sugar levels by about 1.71- to 1.88-fold and 2.26- to 2.48-fold, respectively. Although Xyl10g has low specific activity for beechwood xylan, as compared to XynA, Xyl10g more efficiently degraded corn stover than did XynA. According to immunogold labeling analysis, Xyl10g can attack highly substituted, unsubstituted, and low-substituted xylans, whereas XynA cannot efficiently attack highly substituted xylans, which is important for lignocellulose degradation. These results suggest that GH10 Xyl10g can be used for lignocellulose degradation.
authors.affiliation: Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Ho Myeong
authors.initials: HM
authors.lastname: Kim
authors.affiliation: Department of Wood Science and Landscape Architecture, Chonnam National University, Gwangju 500-757, Republic of Korea; Center for Research Facilities, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Kwang Ho
authors.initials: KH
authors.lastname: Lee
authors.affiliation: Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Kyoung Hyoun
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Department of Wood Science and Landscape Architecture, Chonnam National University, Gwangju 500-757, Republic of Korea; Bio-Energy Research Center, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Dae-Seok
authors.initials: DS
authors.lastname: Lee
authors.affiliation: Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Quynh Anh
authors.initials: QA
authors.lastname: Nguyen
authors.affiliation: Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea; Department of Wood Science and Landscape Architecture, Chonnam National University, Gwangju 500-757, Republic of Korea; Bio-Energy Research Center, Chonnam National University, Gwangju 500-757, Republic of Korea. Electronic address: baehj@chonnam.ac.kr.
authors.forename: Hyeun-Jong
authors.initials: HJ
authors.lastname: Bae
mesh_terms: D001487:Basidiomycota / Q000145:classification / Q000201:enzymology*; D003001:Cloning, Molecular; D029964:Fagus / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D014908:Triticum / Q000378:metabolism; D014990:Xylans / Q000378:metabolism*; D014995:Xylosidases / Q000235:genetics / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D011994:Recombinant Proteins; D014990:Xylans; C036909:lignocellulose; D008031:Lignin; D014995:Xylosidases
keywords: Gloeophyllum trabeum; Synergistic effect; Xylanase