title: [Regulation of E-cadherin promoter activity by LMO2 impact on the progression and metastasis of prostate cancer].
abstract: OBJECTIVE
  To study the abnormal expression of the proto-oncogene LMO2 affect the progression and metastasis mechanism of prostate cancer.
  
  
  METHODS
  A series of reporter gene expression vectors carrying different lengths and point mutations of E-cadherin promoter were constructed. These plasmids were separately co-transfected with LMO2 into Lncap cells and the luciferase activity was detected after 24 h.
  
  
  RESULTS
  The overexpression of LMO2 could significantly inhibit the activity of luciferase reporter gene of E-cadherin promoter about 50%. Truncated and point mutation study showed that this was mainly through E-box sites in the promoter region -204/-198.
  
  
  CONCLUSIONS
  The proto-oncogene LMO2 can affect the progression and metastasis mechanism of prostate cancer by transcriptional inhibition of E-cadherin.
authors.affiliation: Medical College, Nankai University, Tianjin 300451, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Peng
authors.affiliation: Medical College, Nankai University, Tianjin 300451, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ye
authors.affiliation: Medical College, Nankai University, Tianjin 300451, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D015703:Antigens, CD; D015820:Cadherins / Q000235:genetics*; D045744:Cell Line, Tumor; D005822:Genetic Vectors; D006801:Humans; D060588:LIM Domain Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009362:Neoplasm Metastasis; D017354:Point Mutation; D011401:Promoter Regions, Genetic; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D060588:LIM Domain Proteins; C085117:LMO2 protein, human; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins