title: Introduction of the rd29A:AtDREB2A CA gene into soybean (Glycine max L. Merril) and its molecular characterization in leaves and roots during dehydration.
abstract: The loss of soybean yield to Brazilian producers because of a water deficit in the 2011-2012 season was 12.9%. To reduce such losses, molecular biology techniques, including plant transformation, can be used to insert genes of interest into conventional soybean cultivars to produce lines that are more tolerant to drought. The abscisic acid (ABA)-independent Dehydration Responsive Element Binding (DREB) gene family has been used to obtain plants with increased tolerance to abiotic stresses. In the present study, the rd29A:AtDREB2A CA gene from Arabidopsis thaliana was inserted into soybean using biolistics. Seventy-eight genetically modified (GM) soybean lines containing 2-17 copies of the AtDREB2A CA gene were produced. Two GM soybean lines (P1397 and P2193) were analyzed to assess the differential expression of the AtDREB2A CA transgene in leaves and roots submitted to various dehydration treatments. Both GM lines exhibited high expression of the transgene, with the roots of P2193 showing the highest expression levels during water deficit. Physiological parameters examined during water deficit confirmed the induction of stress. This analysis of AtDREB2A CA expression in GM soybean indicated that line P2193 had the greatest stability and highest expression in roots during water deficit-induced stress.
authors.affiliation: Departamento de Genética e Biologia Molecular, Universidade Estadual de Londrina, PR, Brazil .
authors.forename: Cibelle
authors.initials: C
authors.lastname: Engels
authors.affiliation: Laboratório de Biotecnologia Vegetal, Embrapa Soja, Londrina, PR, Brazil .
authors.forename: Renata
authors.initials: R
authors.lastname: Fuganti-Pagliarini
authors.affiliation: Laboratório de Biotecnologia Vegetal, Embrapa Soja, Londrina, PR, Brazil .
authors.forename: Silvana Regina Rockenbach
authors.initials: SR
authors.lastname: Marin
authors.affiliation: Laboratório de Biotecnologia Vegetal, Embrapa Soja, Londrina, PR, Brazil .
authors.forename: Francismar Corrêa
authors.initials: FC
authors.lastname: Marcelino-Guimarães
authors.affiliation: Laboratório de Biotecnologia Vegetal, Embrapa Soja, Londrina, PR, Brazil .
authors.forename: Maria Cristina Neves
authors.initials: MC
authors.lastname: Oliveira
authors.affiliation: Biological Resources and Post-Harvest Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki, Japan .
authors.forename: Norihito
authors.initials: N
authors.lastname: Kanamori
authors.affiliation: Laboratory of Plant Molecular Physiology, The University of Tokyo, Tokyo, Japan .
authors.forename: Junya
authors.initials: J
authors.lastname: Mizoi
authors.affiliation: Biological Resources and Post-Harvest Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki, Japan .
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nakashima
authors.affiliation: Biological Resources and Post-Harvest Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki, Japan . ; Laboratory of Plant Molecular Physiology, The University of Tokyo, Tokyo, Japan .
authors.forename: Kazuko
authors.initials: K
authors.lastname: Yamaguchi-Shinozaki
authors.affiliation: Laboratório de Biotecnologia Vegetal, Embrapa Soja, Londrina, PR, Brazil .
authors.forename: Alexandre Lima
authors.initials: AL
authors.lastname: Nepomuceno
keywords: Arabidopsis; differential expression; genetically modified organism; water deficit