title: Molecular cloning and characterization of SOCS-2 from Manila clam Ruditapes philippinarum.
abstract: Suppressor of cytokine signaling (SOCS) family members are key regulators of immunological homeostasis. In this study, we have discovered the SOCS-2 member from Manila clam Ruditapes philippinarum and further analyzed its immune responses against lipopolysaccharide (LPS) and polyinosinic:polycytidylic acid (poly I:C). Amino acid sequence of RpSOCS-2 consists of cytokine inducible SRC homology 2 (SH2) and SOCS box domains similar to vertebrate SOCS counterparts. It has the highest amino acid identity (41%) with Pacific oyster (Crassostrea gigas) SOCS-2 and showed close evolutional relationship with disk abalone (Haliotis discus discus) SOCS-2. Tissue specific expression results showed that RpSOCS-2 was constitutively expressed in all examined tissues with the highest level in gill tissue of un-challenged clams. RpSOCS-2 mRNA expression was up-regulated by LPS and poly I:C challenge in gills. Discovery of RpSOCS-2 homologue and expression analysis would support for understanding evolutional relationships and their role in innate immune responses in mollusks, respectively.
authors.affiliation: Korea Institute of Ocean Science Technology, Ansan 426-744, Republic of Korea.
authors.forename: Youngdeuk
authors.initials: Y
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungnam National University, Yuseong-gu, Daejeon 305-764, Republic of Korea.
authors.forename: Jin-Young
authors.initials: JY
authors.lastname: Choi
authors.affiliation: Korea Institute of Ocean Science Technology, Ansan 426-744, Republic of Korea.
authors.forename: Chulhong
authors.initials: C
authors.lastname: Oh
authors.affiliation: Korea Institute of Ocean Science Technology, Ansan 426-744, Republic of Korea.
authors.forename: Do-Hyoung
authors.initials: DH
authors.lastname: Kang
authors.affiliation: Korea Local Education Institute, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Su-Young
authors.initials: SY
authors.lastname: Choi
authors.affiliation: College of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Gang-Joon
authors.initials: GJ
authors.lastname: Heo
authors.affiliation: Department of Marine Life Sciences, School of Marine Biomedical Science, Jeju National University, Ara-Dong, Jeju Special Self-Governing Province 690-756, Republic of Korea. Electronic address: jehee@jejunu.ac.kr.
authors.forename: Jehee
authors.initials: J
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungnam National University, Yuseong-gu, Daejeon 305-764, Republic of Korea. Electronic address: mahanama@cnu.ac.kr.
authors.forename: Mahanama
authors.initials: M
authors.lastname: De Zoysa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000502:physiology; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D016415:Sequence Alignment; D050826:Suppressor of Cytokine Signaling Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D050826:Suppressor of Cytokine Signaling Proteins
keywords: LPS; Manila clam; Ruditapes philippinarum; Suppressor of cytokine signaling (SOCS); poly I:C