title: Msd1/SSX2IP-dependent microtubule anchorage ensures spindle orientation and primary cilia formation.
abstract: Anchoring microtubules to the centrosome is critical for cell geometry and polarity, yet the molecular mechanism remains unknown. Here we show that the conserved human Msd1/SSX2IP is required for microtubule anchoring. hMsd1/SSX2IP is delivered to the centrosome in a centriolar satellite-dependent manner and binds the microtubule-nucleator γ-tubulin complex. hMsd1/SSX2IP depletion leads to disorganised interphase microtubules and misoriented mitotic spindles with reduced length and intensity. Furthermore, hMsd1/SSX2IP is essential for ciliogenesis, and during zebrafish embryogenesis, knockdown of its orthologue results in ciliary defects and disturbs left-right asymmetry. We propose that the Msd1 family comprises conserved microtubule-anchoring proteins.
authors.affiliation: Laboratory of Cell Regulation UK, London Research Institute, London, UK.
authors.forename: Akiko
authors.initials: A
authors.lastname: Hori
authors.forename: Chiho
authors.initials: C
authors.lastname: Ikebe
authors.forename: Masazumi
authors.initials: M
authors.lastname: Tada
authors.forename: Takashi
authors.initials: T
authors.lastname: Toda
mesh_terms: D000818:Animals; D002923:Cilia / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D008941:Spindle Apparatus / Q000378:metabolism*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D012097:Repressor Proteins; D029961:Zebrafish Proteins; C093962:synovial sarcoma X breakpoint proteins