title: Function of mGlu1 receptors in the modulation of nociceptive processing in the thalamus.
abstract: As postsynaptic metabotropic subtype 1 (mGlu1) receptors are present in the thalamus, we have investigated the effect of potentiating and antagonising mGlu1 receptors on responses of thalamic neurones to noxious sensory stimulation. Extracellular recordings were made in vivo with multi-barrel iontophoretic electrodes from single neurones in the thalamus of urethane-anaesthetised rats. Responses to iontophoretic applications of the Group I mGlu agonist 3,5-dihydroxy-phenylglycine (DHPG) were selectively potentiated by co-application of the mGlu1 positive allosteric modulator Ro67-4853, whereas they were selectively reduced upon co-application of the mGlu1 receptor orthosteric antagonist LY367385. This indicates that thalamic DHPG responses are mediated primarily via mGlu1 receptors, consistent with the high postsynaptic levels of this receptor in the thalamus. Furthermore, potentiation of DHPG responses by Ro67-4853 were greater when the initial DHPG response was of a low magnitude. Ro67-4853 also potentiated responses of thalamic neurones to noxious thermal stimulation, whilst having little effect on the baseline activity of nociceptive neurones. By contrast, nociceptive responses were reduced by LY367385. In a further series of experiments we found that inactivation of somatosensory cortex by cooling resulted in a reduction of thalamic nociceptive responses. These results underline the importance of mGlu1 receptors in the processing of sensory information in the thalamus, particularly with respect to nociceptive responses. Furthermore, the involvement of mGlu1 receptors may reflect the activity of descending cortico-thalamic afferents.
authors.affiliation: Visual Neuroscience, UCL Institute of Ophthalmology, London EC1V 9EL, United Kingdom. Electronic address: t.salt@ucl.ac.uk.
authors.forename: T E
authors.initials: TE
authors.lastname: Salt
authors.affiliation: Visual Neuroscience, UCL Institute of Ophthalmology, London EC1V 9EL, United Kingdom.
authors.forename: H E
authors.initials: HE
authors.lastname: Jones
authors.affiliation: Visual Neuroscience, UCL Institute of Ophthalmology, London EC1V 9EL, United Kingdom.
authors.forename: C S
authors.initials: CS
authors.lastname: Copeland
authors.affiliation: Visual Neuroscience, UCL Institute of Ophthalmology, London EC1V 9EL, United Kingdom.
authors.forename: A M
authors.initials: AM
authors.lastname: Sillito
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001565:Benzoates / Q000494:pharmacology; D002219:Carbamates / Q000494:pharmacology; D003080:Cold Temperature; D018683:Excitatory Amino Acid Agents / Q000494:pharmacology; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D006358:Hot Temperature; D008297:Male; D009434:Neural Pathways / Q000503:physiopathology; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D059225:Nociception / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D018094:Receptors, Metabotropic Glutamate / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012118:Resorcinols / Q000494:pharmacology; D013003:Somatosensory Cortex / Q000503:physiopathology; D013788:Thalamus / Q000187:drug effects / Q000502:physiology*; D014966:Xanthenes / Q000494:pharmacology
chemical_list: C573813:(9H-xanthene-9-carbonyl)carbamic acid butyl ester; D001565:Benzoates; D002219:Carbamates; D018683:Excitatory Amino Acid Agents; D018690:Excitatory Amino Acid Agonists; D018691:Excitatory Amino Acid Antagonists; D018094:Receptors, Metabotropic Glutamate; D012118:Resorcinols; D014966:Xanthenes; C104077:metabotropic glutamate receptor type 1; C079694:alpha-methyl-4-carboxyphenylglycine; C079215:3,5-dihydroxyphenylglycine; D005998:Glycine
keywords: Cortico-thalamic; Metabotropic glutamate receptors; Nociception; Pain; Thalamus; mGlu1