title: Cell biological analyses of anther morphogenesis and pollen viability in Arabidopsis and rice.
abstract: Major advances have been made in recent years in our understanding of anther development through a combination of genetic studies, cell biological technologies, biochemical analysis, microarray and high-throughput sequencing-based approaches. In this chapter, we summarize the widely used protocols for pollen viability staining; the investigation of anther morphogenesis by light microscopy of semi-thin sections; TUNEL assay for programmed tapetum cell death; and laser microdissection procedures to obtain specialized cells or cell layers for carrying out transcriptomics.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Chang
authors.forename: Zaibao
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yue
authors.initials: Y
authors.lastname: Jin
authors.forename: Hong
authors.initials: H
authors.lastname: Ma
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000254:growth & development / Q000502:physiology*; D016923:Cell Death; D002482:Cellulose / Q000378:metabolism; D061346:Laser Capture Microdissection; D009024:Morphogenesis; D012275:Oryza / Q000166:cytology* / Q000254:growth & development / Q000502:physiology*; D011058:Pollen / Q000254:growth & development / Q000502:physiology*; D013194:Staining and Labeling; D014022:Tissue Survival
chemical_list: D002482:Cellulose