title: Neonatal bacillus Calmette-Guerin vaccination and environmental mycobacteria in sensitizing antimycobacterial activity of macrophages.
abstract: An immunoepidemiological study was performed to evaluate the effect of neonatal bacillus Calmette-Guérin (BCG) vaccination and tuberculin response on macrophage killing profile against Mycobacterium tuberculosis. In this epidemiological field work, the study subjects were drawn from in and around Chennai city, South India. The descriptive epidemiological pattern of neonatal BCG vaccination and its impact on tuberculin skin test were studied. The study subjects for the immunologic laboratory experiments were recruited based on the skin test (Mantoux) outcome and were grouped in to 4 natural study groups that include vaccinated reactors, vaccinated nonreactors, nonvaccinated reactors and nonvaccinated nonreactors. In immunologic laboratory work part, the elucidation of macrophage killing profile was studied for all the 4 groups, and appropriate intercomparisons were made. The parameters used for the macrophage killing profile were (1) glutathione assay, (2) measurement of phagocytosis, (3) intracellular growth kinetics of M. tuberculosis H37Rv, (4) tumor necrosis factor-α assay and (5) interferon-γ assay. The results found that in the BCG-vaccinated tuberculin reactors the macrophage responses were significantly higher than the BCG-vaccinated tuberculin nonreactors. There was no significant difference in the responses among the BCG-vaccinated tuberculin reactors when compared with the nonvaccinated tuberculin reactors. The immune responses of nonvaccinated tuberculin reactors were significantly higher than the vaccinated tuberculin nonreactors. These findings show that the immune response among the adolescents/young adults is elicited by exposure to mycobacteria and not by the neonatal BCG vaccination.
authors.affiliation: Department of Biotechnology (MP), Sree Sastha Institute of Engineering and Technology, Chennai, India; Department of Epidemiology (MD), The Tamil Nadu Dr. M.G.R. Medical University, Chennai, India; and Departments of Biochemistry (MK), Pathology (VDR), and Statistics (PV), National Institute for Research in Tuberculosis (Formerly Tuberculosis Research Center), Indian Council of Medical Research, Chennai, India.
authors.forename: Mathan
authors.initials: M
authors.lastname: Periasamy
authors.forename: Manjula
authors.initials: M
authors.lastname: Datta
authors.forename: M
authors.initials: M
authors.lastname: Kannapiran
authors.forename: V D
authors.initials: VD
authors.lastname: Ramanathan
authors.forename: Perumal
authors.initials: P
authors.lastname: Venkatesan
mesh_terms: D000293:Adolescent; D001500:BCG Vaccine / Q000276:immunology / Q000627:therapeutic use*; D002478:Cells, Cultured; D003430:Cross-Sectional Studies; D004781:Environmental Exposure / Q000517:prevention & control*; D006801:Humans; D007111:Immunity, Cellular; D007194:India / Q000453:epidemiology; D007231:Infant, Newborn; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology; D008297:Male; D009163:Mycobacterium bovis / Q000276:immunology / Q000302:isolation & purification*; D014376:Tuberculosis / Q000453:epidemiology / Q000276:immunology / Q000517:prevention & control*; D014611:Vaccination / Q000639:trends*; D055815:Young Adult
chemical_list: D001500:BCG Vaccine