title: Characterization of an antihypertensive angiotensin I-converting enzyme inhibitory peptide from the edible mushroom Hypsizygus marmoreus.
abstract: Hypertension is one of the very serious diseases and, recently, hypertensive patient longevity has been increased significantly. Therefore, the development of new antihypertensive drugs or bioactive compounds is very important to remedy or prevent hypertension. The antihypertensive angiotensin I-converting enzyme (ACE) inhibitor in water extracts from the brown-cultivar-fruiting-body of Hypsizygus marmoreus was purified with ultrafiltration, C18 solid phase extraction chromatography and reverse-phase HPLC, and the purified ACE inhibitor with inhibitory activity of IC50 value of 0.19 mg/mL was obtained. The purified ACE inhibitor was found to be a new oligopeptide with the sequence LSMGSASLSP. Its molecular weight was estimated to be 567.3 Da and the water extracts containing ACE inhibitor from Hypsizygus marmoreus showed a clear antihypertensive action a spontaneously hypertensive rat.
authors.affiliation: Department of Biomedicinal Science and Biotechnology, PaiChai University, Daejeon 302-735, Republic of Korea.
authors.forename: Min-Gu
authors.initials: MG
authors.lastname: Kang
authors.affiliation: Department of Biomedicinal Science and Biotechnology, PaiChai University, Daejeon 302-735, Republic of Korea.
authors.forename: Young-Hun
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Biomedicinal Science and Biotechnology, PaiChai University, Daejeon 302-735, Republic of Korea.
authors.forename: Zanabaatar
authors.initials: Z
authors.lastname: Bolormaa
authors.affiliation: Korea National College of Agricultural and Fishery, Hwasung, Kyonggi-do 445-893, Republic of Korea.
authors.forename: Min-Kyung
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Korea National College of Agricultural and Fishery, Hwasung, Kyonggi-do 445-893, Republic of Korea.
authors.forename: Geon-Sik
authors.initials: GS
authors.lastname: Seo
authors.affiliation: Department of Biomedicinal Science and Biotechnology, PaiChai University, Daejeon 302-735, Republic of Korea.
authors.forename: Jong-Soo
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000363:Agaricales / Q000201:enzymology; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage / Q000737:chemistry*; D001794:Blood Pressure / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006973:Hypertension / Q000188:drug therapy* / Q000473:pathology; D010455:Peptides / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D010455:Peptides; D007703:Peptidyl-Dipeptidase A