title: The substantive equivalence of transgenic (Bt and Chi) and non-transgenic cotton based on metabolite profiles.
abstract: Compositional studies comparing transgenic with non-transgenic counterpart plants are almost universally required by governmental regulatory bodies. In the present study, two T(2) transgenic cotton lines containing chitinase (Line 11/57) and Bt lines (Line 61) were compared with non-transgenic counterpart. To do this, biochemical characteristics of leaves and seeds, including amino acids, fatty acids, carbohydrates, anions, and cations contents of the studied lines were analyzed using GC/MS, high-performance liquid chromatography (HPLC), and ion chromatography (IC) analyzers, respectively. polymerase chain reaction (PCR) and Western blot analyses confirmed the presence and expression of Chi and Bt genes in the studied transgenic lines. Although, compositional analysis of leaves contents confirmed no significant differences between transgenic and non-transgenic counterpart lines, but it was shown that glucose content of chitinase lines, fructose content of transgenic lines (Bt and chitinase) and asparagine and glutamine of chitinase lines were significantly higher than the non-transgenic counterpart plants. Both the transgenic lines (Bt and chitinase) showed significant decrease in the amounts of sodium in comparison to the non-transgenic counterpart plants. The experiments on the seeds showed that histidine, isoleucine, leucine, and phenylalanine contents of all transgenic and non-transgenic lines were the same, whereas other amino acids were significantly increased in the transgenic lines. Surprisingly, it was observed that the concentrations of stearic acid, myristic acid, oleic acid, and linoleic acid in the chitinase line were significantly different than those of non-transgenic counterpart plants, but these components were the same in both Bt line and its non-transgenic counterpart. It seems that more changes observed in the seed contents than leaves is via this point that seeds are known as metabolites storage organs, so they show greater changes in the metabolites contents comparing to the leaves.
authors.affiliation: Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Zanjan, Islamic Republic of Iran.
authors.forename: Bentol Hoda
authors.initials: BH
authors.lastname: Modirroosta
authors.forename: Masoud
authors.initials: M
authors.lastname: Tohidfar
authors.forename: Jalal
authors.initials: J
authors.lastname: Saba
authors.forename: Foad
authors.initials: F
authors.lastname: Moradi
mesh_terms: D000596:Amino Acids / Q000032:analysis / Q000378:metabolism; D000838:Anions / Q000378:metabolism; D002241:Carbohydrates / Q000032:analysis; D002412:Cations / Q000378:metabolism; D002688:Chitinases / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D003368:Gossypium / Q000235:genetics* / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012639:Seeds / Q000235:genetics / Q000378:metabolism
chemical_list: D000596:Amino Acids; D000838:Anions; D002241:Carbohydrates; D002412:Cations; D005227:Fatty Acids; D002688:Chitinases