title: Early growth response protein 1 promotes restenosis by upregulating intercellular adhesion molecule-1 in vein graft.
abstract: OBJECTIVE
  To verify the relationship between Egr-1 and vein graft restenosis and investigate the related mechanisms.
  
  
  METHODS
  Mouse vein graft models were established in Egr-1 knockout (KO) and wild-type (WT) mice. The vein grafts in the mice were taken for pathological examination and immunohistochemical analysis. The endothelial cells (ECs) were stimulated by using a computer-controlled cyclic stress unit. BrdU staining and PCR were used to detect ECs proliferation activity and Egr-1 and ICAM-1 mRNA expression, respectively. Western-blot analysis was also used to detect expression of Egr-1 and intercellular adhesion molecule-1 (ICAM-1) proteins.
  
  
  RESULTS
  The lumens of vein grafts in Egr-1 KO mice were wider than in WT mice. ECs proliferation after mechanical stretch stimulation was suppressed by Egr-1 knockout (P < 0.05). Both in vein grafts and ECs from WT mice after mechanical stretch stimulation, mRNA expression and protein of Egr-1 and ICAM-1 showed increases (P < 0.05). However, ICAM-1 expression was significantly suppressed in ECs from Egr-1 knockout mice (P < 0.05).
  
  
  CONCLUSIONS
  Egr-1 may promote ECs proliferation and result in vein graft restenosis by upregulating the expression of ICAM-1. As a key factor of vein graft restenosis, it could be a target for the prevention of restenosis after CABG surgery.
authors.affiliation: Cardiac Surgery, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing Anzhen Hospital Affiliated with Capital Medical University, Beijing 100029, China.
authors.forename: Kui
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Cardiac Surgery, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing Anzhen Hospital Affiliated with Capital Medical University, Beijing 100029, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Cao
authors.affiliation: Cardiac Surgery, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing Anzhen Hospital Affiliated with Capital Medical University, Beijing 100029, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Dong
authors.affiliation: Vessel Biology, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing Anzhen Hospital Affiliated with Capital Medical University, Beijing 100029, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Du
mesh_terms: D000818:Animals; D001807:Blood Vessel Prosthesis; D023903:Coronary Restenosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D051766:Early Growth Response Protein 1 / Q000235:genetics / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013314:Stress, Mechanical; D015854:Up-Regulation
chemical_list: D051766:Early Growth Response Protein 1; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1