title: The phosphoenolpyruvate phosphotransferase system in group A Streptococcus acts to reduce streptolysin S activity and lesion severity during soft tissue infection.
abstract: Obtaining essential nutrients, such as carbohydrates, is an important process for bacterial pathogens to successfully colonize host tissues. The phosphoenolpyruvate phosphotransferase system (PTS) is the primary mechanism by which bacteria transport sugars and sense the carbon state of the cell. The group A streptococcus (GAS) is a fastidious microorganism that has adapted to a variety of niches in the human body to elicit a wide array of diseases. A ΔptsI mutant (enzyme I [EI] deficient) generated in three different strains of M1T1 GAS was unable to grow on multiple carbon sources (PTS and non-PTS). Complementation with ptsI expressed under its native promoter in single copy was able to rescue the growth defect of the mutant. In a mouse model of GAS soft tissue infection, all ΔptsI mutants exhibited a significantly larger and more severe ulcerative lesion than mice infected with the wild type. Increased transcript levels of sagA and streptolysin S (SLS) activity during exponential-phase growth was observed. We hypothesized that early onset of SLS activity would correlate with the severity of the lesions induced by the ΔptsI mutant. In fact, infection of mice with a ΔptsI sagB double mutant resulted in a lesion comparable to that of either the wild type or a sagB mutant alone. Therefore, a functional PTS is not required for subcutaneous skin infection in mice; however, it does play a role in coordinating virulence factor expression and disease progression.
authors.affiliation: Department of Cell Biology & Molecular Genetics and Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland, USA.
authors.forename: Kanika
authors.initials: K
authors.lastname: Gera
authors.forename: Tuquynh
authors.initials: T
authors.lastname: Le
authors.forename: Rebecca
authors.initials: R
authors.lastname: Jamin
authors.forename: Zehava
authors.initials: Z
authors.lastname: Eichenbaum
authors.forename: Kevin S
authors.initials: KS
authors.lastname: McIver
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002241:Carbohydrates / Q000276:immunology; D005098:Exotoxins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics / Q000276:immunology; D051379:Mice; D009154:Mutation / Q000235:genetics / Q000276:immunology; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D018461:Soft Tissue Infections / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D013207:Staphylococcal Skin Infections / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D013290:Streptococcal Infections / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D013301:Streptolysins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014774:Virulence / Q000235:genetics / Q000276:immunology; D037521:Virulence Factors / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002241:Carbohydrates; D005098:Exotoxins; D013301:Streptolysins; D037521:Virulence Factors; C027951:erythrogenic toxin; C026050:streptolysin S; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System