title: Complete nucleotide sequence of a conjugative IncF plasmid from an Escherichia coli isolate of equine origin containing blaCMY-2 within a novel genetic context.
abstract: A blaCMY-2 -containing conjugative IncF plasmid denoted as pEQ011, previously identified in a multidrug-resistant Escherichia coli isolate of equine origin, was characterized. The plasmid consisted of 85 507 bp, with 118 predicted open reading frames. This is the first known report demonstrating the association of a blaCMY-2 gene with an IncF incompatibility-type plasmid backbone. A novel genetic arrangement was identified wherein the blaCMY-2 resistance gene was proximally flanked by IS1294 along with a partial blc gene located distally and within a yacABC operon.
authors.affiliation: UCD Centre for Molecular Innovation & Drug Discovery, School of Public Health, Physiotherapy & Population Science, University College Dublin, Belfield, Dublin, Ireland.
authors.forename: Maria
authors.initials: M
authors.lastname: Karczmarczyk
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Nola
authors.initials: N
authors.lastname: Leonard
authors.forename: Séamus
authors.initials: S
authors.lastname: Fanning
mesh_terms: D000818:Animals; D001483:Base Sequence; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D023061:Gene Order; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: C103022:beta-lactamase CMY-2; D001618:beta-Lactamases
keywords: Enterobacteriaceae; ampC; horses; plasmid-mediated β-lactam resistance