title: A new concept in hybridization: Bromelain enzyme for deproteinizing dentin before application of adhesive system.
abstract: OBJECTIVE
  The objective of this study is to assess the deproteinizing effect of bromelain enzyme and compare it with neodymium-doped yttrium aluminum garnet (Nd:YAG) laser and 10% sodium hypochlorite (NaOCl) by using scanning electron microscope (SEM) and polarized microscope.
  
  
  METHODS
  A total of 60 extracted human upper premolars were selected to be given standardized buccal and lingual class V cavities. The teeth were divided into three groups each one consisted of 20 teeth. Thirty teeth were recruited for SEM study and the other 30 for polarized microscope. Group 1: Teeth were deproteinized with Nd:YAG laser, Group 2: Teeth were deproteinized with bromelain enzyme and Group 3: Teeth were deproteinized with 10% NaOCl.
  
  
  CONCLUSIONS
  Application of bromelain enzyme has led to removing collagen network and significantly decreased the global leakage scores of the adhesive system.
authors.affiliation: Professor Dr. Raad Niama Dayem BDS, MSc, PhD Conservative Dentistry, USA.
authors.forename: Raad Niama
authors.initials: RN
authors.lastname: Dayem
authors.affiliation: Professor Dr. Raad Niama Dayem BDS, MSc, PhD Conservative Dentistry, USA.
authors.forename: Mona Adnan
authors.initials: MA
authors.lastname: Tameesh
keywords: Adhesive system; bromelain enzyme; deproteinizing dentin