title: DNA double strand break end-processing and RecA induce RecN expression levels in Bacillus subtilis.
abstract: Bacillus subtilis cells respond to double strand breaks (DSBs) with an ordered recruitment of repair proteins to the site lesion, being RecN one of the first responders. In B. subtilis, one of the responses to DSBs is to increase RecN expression rather than modifying its turnover rate. End-processing activities and the RecA protein itself contribute to increase RecN levels after DNA DSBs. RecO is required for RecA filament formation and full SOS induction, but its absence did not significantly affect RecN expression. Neither the absence of LexA nor the phosphorylation state of RecA or SsbA significantly affect RecN expression levels. These findings identify two major mechanisms (SOS and DSB response) used to respond to DSBs, with LexA required for one of them (SOS response). The DSB response, which requires end-processing and RecA or short RecO-independent RecA filaments, highlights the importance of guarding genome stability by modulating the DNA damage responses.
authors.affiliation: Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, CSIC, Darwin 3, 28049 Madrid, Spain.
authors.forename: Paula P
authors.initials: PP
authors.lastname: Cardenas
authors.affiliation: Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, CSIC, Darwin 3, 28049 Madrid, Spain.
authors.forename: Carolina
authors.initials: C
authors.lastname: Gándara
authors.affiliation: Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, CSIC, Darwin 3, 28049 Madrid, Spain. Electronic address: jcalonso@cnb.csic.es.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Alonso
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D053903:DNA Breaks, Double-Stranded / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004790:Enzyme Induction / Q000187:drug effects; D019848:Gene Expression Regulation, Archaeal / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016685:Mitomycin / Q000494:pharmacology; D011938:Rec A Recombinases / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D016685:Mitomycin; D006861:Hydrogen Peroxide; D011938:Rec A Recombinases; D004262:DNA Restriction Enzymes; C047879:RecN protein, Bacteria
keywords: AddAB; DNA damage response; Double strand breaks; LexA; RecA; RecJ; RecN; SOS response