title: Supplementation with conjugated linoleic acids extends the adiponectin deficit during early lactation in dairy cows.
abstract: Decreasing insulin sensitivity (IS) in peripheral tissues allows for partitioning nutrients towards the mammary gland. In dairy cows, extensive lipid mobilization and continued insulin resistance (IR) are typical for early lactation. Adiponectin, an adipokine, promotes IS. Supplementation with conjugated linoleic acids (CLA) in rodents and humans reduces fat mass whereby IR and hyperinsulinemia may occur. In dairy cows, CLA reduce milk fat, whereas body fat, serum free fatty acids and leptin are not affected. We aimed to investigate the effects of CLA supplementation on serum and adipose tissue (AT) adiponectin concentrations in dairy cows during the lactation driven and parity modulated changes of metabolism. High yielding cows (n=33) were allocated on day 1 post partum to either 100 g/day of a CLA mixture or a control fat supplement (CON) until day 182 post partum. Blood and subcutaneous (sc) AT (AT) biopsy samples were collected until day 252 post partum to measure adiponectin. Serum adiponectin decreased from day 21 pre partum reaching a nadir at calving and thereafter increased gradually. The distribution of adiponectin molecular weight forms was neither affected by time, parity nor treatment. Cows receiving CLA had decreased serum adiponectin concentrations whereby primiparous cows responded about 4 weeks earlier than multiparous cows. The time course of adiponectin concentrations in sc AT (corrected for residual blood) was similar to serum concentrations, without differences between CLA and CON. CLA supplementation attenuated the post partum increase of circulating adiponectin thus acting towards prolongation of peripartal IR and drain of nutrients towards the mammary gland.
authors.affiliation: Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, 53115 Bonn, Germany.
authors.forename: Shiva P
authors.initials: SP
authors.lastname: Singh
authors.affiliation: Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, 53115 Bonn, Germany. Electronic address: susanne.haeussler@uni-bonn.de.
authors.forename: Susanne
authors.initials: S
authors.lastname: Häussler
authors.affiliation: Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, 53115 Bonn, Germany.
authors.forename: Johanna F L
authors.initials: JF
authors.lastname: Heinz
authors.affiliation: Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, 53115 Bonn, Germany.
authors.forename: Behnam
authors.initials: B
authors.lastname: Saremi
authors.affiliation: Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, 53115 Bonn, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Mielenz
authors.affiliation: Clinic for Cattle, University of Veterinary Medicine, 30173 Hannover, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Rehage
authors.affiliation: Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, 38116 Braunschweig, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Dänicke
authors.affiliation: Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, 53115 Bonn, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Mielenz
authors.affiliation: Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, 53115 Bonn, Germany.
authors.forename: Helga
authors.initials: H
authors.lastname: Sauerwein
mesh_terms: D052242:Adiponectin / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D019587:Dietary Supplements; D005260:Female; D007774:Lactation / Q000187:drug effects* / Q000378:metabolism*; D020738:Leptin / Q000378:metabolism; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage* / Q000494:pharmacology; D008892:Milk / Q000378:metabolism*; D010298:Parity / Q000187:drug effects; D049590:Postpartum Period / Q000187:drug effects; D011247:Pregnancy
chemical_list: D052242:Adiponectin; D020738:Leptin; D044243:Linoleic Acids, Conjugated
keywords: Adiponectin; Conjugated linoleic acids; Dairy cow; Lactation; Parity