title: Genetic variability in the ITS and IGS regions of the ribosomal DNA of Acremonium cavaraeanum exhibiting antimicrobial activity.
abstract: Endophytic microorganisms represent promising alternatives for obtaining new drugs of biotechnological importance. In this study, the endophytic species Acremonium cavaraeanum (A1a) isolated from Cocos nucifera was cultivated for the production of secondary metabolites, and its extracts and fractions were evaluated by the dilution method (MIC). The EtOAc extracts and MeOH fractions were tested against Gram-positive and -negative bacteria, and had an MIC of 125 µg/mL when evaluated in the EtOAc extract (EBI). The EtOAc extract (EBII) had an MIC of 62.25 µg/mL for Staphylococcus aureus and an MIC between 125 and 250 µg/mL for Gram-negative bacteria. The methanolic fractions showed activity with MIC between 125 and 250 µg/mL for all bacteria tested. The IGS region of the rDNA repeat unit of genomic DNA was analyzed by PCR/RFLPs, including endonucleases PstI, BamHII, HinfI, and EcoRI. The physical maps showed different restriction sites for the 6 Acremonium sp isolates, and revealed 5 RFLP patterns. The results showed that isolates of the same Acremonium species exhibited variation in this specific region. The sequences of ITS1-5.8S-ITS2 regions were aligned by Clustal W using the neighbor joining method, which grouped the isolates into 5 distinct clusters. This study aimed to evaluate the genetic diversity of A. cavaraeanum crops exhibiting antibacterial activity. The results of this study indicate that different fungal genetic isolates have biotechnological potential for the production of active bio-compounds against several human pathogens.
authors.affiliation: Laboratório de Genética Molecular, Genômica e Proteômica, Centro de Ciências Agrárias, Campus Delza Gitaí, Universidade Federal de Alagoas, Rio Largo, AL, Brasil a.todaro@gmail.com.
authors.forename: A R
authors.initials: AR
authors.lastname: Todaro
authors.forename: V X
authors.initials: VX
authors.lastname: Nascimento
authors.forename: N C C
authors.initials: NC
authors.lastname: Souza
authors.forename: P P
authors.initials: PP
authors.lastname: Silva
authors.forename: J M
authors.initials: JM
authors.lastname: Santos
authors.forename: E A V F
authors.initials: EA
authors.lastname: Ramalho
authors.forename: T V C
authors.initials: TV
authors.lastname: Melo
authors.forename: I S M
authors.initials: IS
authors.lastname: Silva
authors.forename: G S
authors.initials: GS
authors.lastname: Lins Júnior
authors.forename: M L A
authors.initials: ML
authors.lastname: Bastos
authors.forename: D F
authors.initials: DF
authors.lastname: Farias
authors.forename: P C V
authors.initials: PC
authors.lastname: Souza
authors.forename: A F U
authors.initials: AF
authors.lastname: Carvalho
authors.forename: M C S
authors.initials: MC
authors.lastname: Silva
authors.forename: R M R G
authors.initials: RM
authors.lastname: Almeida
authors.forename: E
authors.initials: E
authors.lastname: Ramalho Neto
mesh_terms: D000164:Acremonium / Q000235:genetics* / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000302:isolation & purification; D002874:Chromosome Mapping; D003059:Cocos / Q000382:microbiology; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D005656:Fungal Proteins / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D059625:Liquid-Liquid Extraction; D008826:Microbial Sensitivity Tests; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D000900:Anti-Bacterial Agents; D021903:DNA, Ribosomal Spacer; D005656:Fungal Proteins