title: Isolation and Assessment of Mesenchymal Stem Cells Derived From Bone Marrow: Histologic and Histomorphometric Study in a Canine Periodontal Defect.
abstract: The aim of the present study was to investigate an isolation procedure to culture mesenchymal stem cells derived from bone marrow and evaluate their potential in periodontal regeneration. Potential stem cells from bone marrow, aspirated from the iliac crest of nine mongrel canines 1 to 2 years of age, were cultivated. After the examination of surface epitopes of the isolated cells, the total RNA from osteogenic, adipogenic, and chondrogenic cell cultures were analyzed by reverse transcription polymerase chain reaction (RT-PCR) to confirm stem cell gene expressions. 2 × 10(7) mL of the stem cells were loaded on 0.2 mL of anorganic bovine bone mineral (ABBM) granules. In each animal, bilateral acute/chronic intrabony periodontal defects were created surgically and by placement of ligatures around the cervical aspect of the teeth. At week 5, after flap debridement, the bilateral defects were randomly assigned to 2 treatment groups: the control group received ABBM, and the test group received BMSCs-loaded ABBM. Eight weeks after transplantation, regenerative parameters were analyzed histologically and histometrically. The RNA expressions confirmed the cultivation of mesenchymal stem cell. More new cementum and periodontal ligament (PDL) were measured in the test group (cementum: 3.33 ± 0.94 vs 2.03 ± 1.30, P = 0.027; PDL: 2.69 ± 0.73 vs 1.53 ± 1.21, P = 0.026). New bone formation was similar in both groups (2.70 ± 0.86 vs 1.99 ± 1.31; P = 0.193). Mesenchymal stem cells derived from bone marrow should be considered a promising technique for use in patients with periodontal attachment loss and merits further investigations.
authors.affiliation: 1  Dental Research Center, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mojgan
authors.initials: M
authors.lastname: Paknejad
authors.affiliation: 2  Department of Stem Cell and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
authors.forename: Mohamadreza Baghaban
authors.initials: MB
authors.lastname: Eslaminejad
authors.affiliation: 3  Department of Periodontics, Qazvin University of Medical Sciences, Qazvin, Iran.
authors.forename: Baharak
authors.initials: B
authors.lastname: Ghaedi
authors.affiliation: 4  Dental Implant Research Center, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Amir-Reza
authors.initials: AR
authors.lastname: Rokn
authors.affiliation: 5  Department of Periodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Afshin
authors.initials: A
authors.lastname: Khorsand
authors.affiliation: 1  Dental Research Center, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Shahroo
authors.initials: S
authors.lastname: Etemad-Moghadam
authors.affiliation: 1  Dental Research Center, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mojgan
authors.initials: M
authors.lastname: Alaeddini
authors.affiliation: 6  Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Dehghan
authors.affiliation: 5  Department of Periodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.|7  Laser Research Center of Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Neda
authors.initials: N
authors.lastname: Moslemi
authors.affiliation: 8  Taipei Academy of Reconstructive Dentistry, Research Expert, the Italian Ministry of Education and Research.
authors.forename: Hessam
authors.initials: H
authors.lastname: Nowzari
mesh_terms: D016301:Alveolar Bone Loss; D000818:Animals; D001853:Bone Marrow; D001861:Bone Regeneration; D002417:Cattle; D003739:Dental Cementum; D016557:Guided Tissue Regeneration, Periodontal; D059630:Mesenchymal Stem Cells; D010513:Periodontal Ligament; D011897:Random Allocation; D012038:Regeneration
keywords: animal; dental cementum; mesenchymal stem cell transplantation; models; periodontal ligament; regeneration; tissue engineering