title: Cellular functions of ascorbic acid: a means to determine vitamin C requirements.
abstract: Optimal ascorbic acid (vitamin C) requirements in humans are unknown. In situ kinetics is a biochemical approach to determine requirements for vitamin C and other vitamins. In situ kinetics requires that cellular functions of ascorbic acid are characterized. Vitamin-C-dependent cellular reactions are directly related to vitamin C concentrations inside and outside cells. By coupling intracellular and extracellular functions of ascorbic acid to vitamin concentration, in situ kinetics provides a novel approach to determining vitamin C requirements.
authors.affiliation: Laboratory of Cell Biology and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: M
authors.initials: M
authors.lastname: Levine
authors.forename: K R
authors.initials: KR
authors.lastname: Dhariwal
authors.forename: P W
authors.initials: PW
authors.lastname: Washko
authors.forename: R W
authors.initials: RW
authors.lastname: Welch
authors.forename: Y
authors.initials: Y
authors.lastname: Wang