title: Nephroprotective effect of date fruit extract against dichloroacetic acid exposure in adult rats.
abstract: The aim of this study was to investigate the protective effects of aqueous date extract (ADE) on dichloroacetic acid (DCA)-induced nephrotoxicity. In vitro, total phenolic content estimated in the ADE were 417.71mg gallic acid equivalents/100g fresh weights (FW), while total flavonoid and tannins contents were 285.23 and 73.65mg catechin equivalents/100g FW, respectively. The ADE has strong scavenging activity. Ferulic, caffeic and p-coumaric acids are the major's compounds. Nephrotoxicity was induced in male Wistar rats by the administration of 0.5 and 2g/L DCA as drinking water. Some of these rats received also by gavage ADE (4mL/kg) before the administration of DCA. After two months of experiment, DCA administration caused elevated levels of renal MDA, significant depletion of GSH levels, altered the antioxidant enzyme activities and deteriorated the renal functions as assessed by the increased plasma urea, uric acid and creatinine levels compared to control rats. The treatment with the ADE significantly normalized the increased plasma levels of creatinine, urea and uric acid, reduced the elevated MDA levels, significantly normalized the antioxidant enzyme activities and GSH level and restored the altered kidney histology in rats treated with DCA. Therefore, it was speculated that ADE protects rats from kidney damage through its antioxidant capacity.
authors.affiliation: Research Unit "Genome, Immune Diagnosis and Recovery", Higher Institute of Biotechnology of Monastir, University of Monastir, Avenue Tahar Hadded, BP 74, 5000 Monastir, Tunisia.
authors.forename: Amira
authors.initials: A
authors.lastname: El Arem
authors.affiliation: Research Unit "Genome, Immune Diagnosis and Recovery", Higher Institute of Biotechnology of Monastir, University of Monastir, Avenue Tahar Hadded, BP 74, 5000 Monastir, Tunisia.
authors.forename: Amira
authors.initials: A
authors.lastname: Thouri
authors.affiliation: Research Unit "Genome, Immune Diagnosis and Recovery", Higher Institute of Biotechnology of Monastir, University of Monastir, Avenue Tahar Hadded, BP 74, 5000 Monastir, Tunisia.
authors.forename: Mouna
authors.initials: M
authors.lastname: Zekri
authors.affiliation: Research Unit "Genome, Immune Diagnosis and Recovery", Higher Institute of Biotechnology of Monastir, University of Monastir, Avenue Tahar Hadded, BP 74, 5000 Monastir, Tunisia.
authors.forename: Emna Behija
authors.initials: EB
authors.lastname: Saafi
authors.affiliation: Laboratory of Biochemistry, Faculty of Medicine Sousse, University of Sousse, Avenue Mohamed Karoui, 99/UR/08-45, 4002 Sousse, Tunisia.
authors.forename: Fatma
authors.initials: F
authors.lastname: Ghrairi
authors.affiliation: Service of Pathological Anatomy CHU F. Bourguiba, Monastir, Tunisia.
authors.forename: Abdelfattah
authors.initials: A
authors.lastname: Zakhama
authors.affiliation: Research Unit "Genome, Immune Diagnosis and Recovery", Higher Institute of Biotechnology of Monastir, University of Monastir, Avenue Tahar Hadded, BP 74, 5000 Monastir, Tunisia. Electronic address: lotfiachour@yahoo.fr.
authors.forename: Lotfi
authors.initials: L
authors.lastname: Achour
mesh_terms: D000818:Animals; D028023:Arecaceae / Q000737:chemistry*; D003999:Dichloroacetic Acid / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010936:Plant Extracts; D003999:Dichloroacetic Acid
keywords: Antioxidant system; Aqueous date fruit extract; Dichloroacetic acid; Histopathology; Renal markers