title: A novel endosomal sorting complex required for transport (ESCRT) component in Arabidopsis thaliana controls cell expansion and development.
abstract: ESCRT proteins mediate membrane remodeling and scission events and are essential for endosomal sorting of plasma membrane proteins for degradation. We have identified a novel, plant-specific ESCRT component called PROS (POSITIVE REGULATOR OF SKD1) in Arabidopsis thaliana. PROS has a strong positive effect on the in vitro ATPase activity of SKD1 (also known as Vacuolar Protein Sorting 4 or VPS4), a critical component required for ESCRT-III disassembly and endosomal vesiculation. PROS interacts with both SKD1 and the SKD1-positive regulator LIP5/VTA1. We have identified a putative MIM domain within PROS that mediate the interaction with the MIT domain of SKD1. Interestingly, whereas MIM domains are commonly found at the C terminus of ESCRT-III subunits, the PROS MIM domain is internal. The heterologous expression of PROS in yeast mutant cells lacking Vta1p partially rescues endosomal sorting defects. PROS is expressed in most tissues and cells types in Arabidopsis thaliana. Silencing of PROS leads to reduced cell expansion and abnormal organ growth.
authors.affiliation: From the Department of Botany, University of Wisconsin-Madison, Madison, Wisconsin 53706.
authors.forename: Francisca C
authors.initials: FC
authors.lastname: Reyes
authors.forename: Rafael A
authors.initials: RA
authors.lastname: Buono
authors.forename: Hannetz
authors.initials: H
authors.lastname: Roschzttardtz
authors.forename: Simone
authors.initials: S
authors.lastname: Di Rubbo
authors.forename: Li Huey
authors.initials: LH
authors.lastname: Yeun
authors.forename: Eugenia
authors.initials: E
authors.lastname: Russinova
authors.forename: Marisa S
authors.initials: MS
authors.lastname: Otegui
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism*; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D049109:Cell Proliferation; D056827:Endosomal Sorting Complexes Required for Transport / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D008969:Molecular Sequence Data; D057146:Multivesicular Bodies / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D063245:Plant Development; D018515:Plant Leaves / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D013045:Species Specificity
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D056827:Endosomal Sorting Complexes Required for Transport; C587925:PROS protein, Arabidopsis; D029701:Saccharomyces cerevisiae Proteins; C107528:VPS4 protein, S cerevisiae; D000251:Adenosine Triphosphatases
keywords: Cell Biology; Cell Growth; Endosomes; Enzyme Purification; Gene Silencing; Membrane Trafficking; Plant; Protein Motifs; Protein Purification