title: Identification of Kashmir bee virus in France using a new RT-PCR method which distinguishes closely related viruses.
abstract: A new RT-PCR protocol has been developed, avoiding potential misdiagnosis of Kashmir bee virus (KBV) linked to the use of KBV primers designed originally. The PCR assay validation was realised taking into account the analytical specificity and the PCR detection limit. KBV was detected in a bee sample collected in France from an apparently healthy apiary in 2012. The specificity of the primers was confirmed by sequencing the PCR product. This French sequence clustered into the KBV genotype by phylogenetic analysis, while previous French sequence isolates collected in 2002 belong to the IAPV genotype. These data represent the first detection of KBV in France.
authors.affiliation: Anses, Sophia-Antipolis Laboratory, Bee Diseases Unit, BP 111, 06902 Sophia Antipolis, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Blanchard
authors.affiliation: Anses, Sophia-Antipolis Laboratory, Bee Diseases Unit, BP 111, 06902 Sophia Antipolis, France.
authors.forename: Jerome
authors.initials: J
authors.lastname: Carletto
authors.affiliation: Bieneninstitut Kirchhain (LLH), Erlenstraβe 9, 35274 Kirchhain, Germany.
authors.forename: Reinhold
authors.initials: R
authors.lastname: Siede
authors.affiliation: Anses, Sophia-Antipolis Laboratory, Bee Diseases Unit, BP 111, 06902 Sophia Antipolis, France.
authors.forename: Frank
authors.initials: F
authors.lastname: Schurr
authors.affiliation: Anses, Sophia-Antipolis Laboratory, Bee Diseases Unit, BP 111, 06902 Sophia Antipolis, France. Electronic address: richard.thiery@anses.fr.
authors.forename: Richard
authors.initials: R
authors.lastname: Thiéry
authors.affiliation: Anses, Sophia-Antipolis Laboratory, Bee Diseases Unit, BP 111, 06902 Sophia Antipolis, France.
authors.forename: Magali
authors.initials: M
authors.lastname: Ribière
mesh_terms: D000818:Animals; D001516:Bees; D017931:DNA Primers / Q000235:genetics; D005602:France; D005838:Genotype; D007304:Insect Viruses / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: D017931:DNA Primers
keywords: Apis mellifera; Kashmir bee virus (KBV); Phylogenetic analysis; RT-PCR