title: Differentiation of human adipose-derived stem cells seeded on mineralized electrospun co-axial poly(ε-caprolactone) (PCL)/gelatin nanofibers.
abstract: Mineralized poly(ε-caprolactone)/gelatin core-shell nanofibers were prepared via co-axial electrospinning and subsequent incubation in biomimetic simulated body fluid containing ten times the calcium and phosphate ion concentrations found in human blood plasma. The deposition of calcium phosphate on the nanofiber surfaces was investigated through scanning electronic microscopy and X-ray diffraction. Energy dispersive spectroscopy results indicated that calcium-deficient hydroxyapatite had grown on the fibers. Fourier transform infrared spectroscopy analysis suggested the presence of hydroxyl-carbonate-apatite. The results of a viability assay (MTT) and alkaline phosphatase activity analysis suggested that these mineralized matrices promote osteogenic differentiation of human adipose-derived stem cells (hASCs) when cultured in an osteogenic medium and have the potential to be used as a scaffold in bone tissue engineering. hASCs cultured in the presence of nanofibers in endothelial differentiation medium showed lower rates of proliferation than cells cultured without the nanofibers. However, endothelial cell markers were detected in cells cultured in the presence of nanofibers in endothelial differentiation medium.
authors.affiliation: Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais (UFMG), Pampulha, Belo Horizonte, MG, Brazil.
authors.forename: Ildeu H L
authors.initials: IH
authors.lastname: Pereira
authors.forename: Eliane
authors.initials: E
authors.lastname: Ayres
authors.forename: Luc
authors.initials: L
authors.lastname: Averous
authors.forename: Guy
authors.initials: G
authors.lastname: Schlatter
authors.forename: Anne
authors.initials: A
authors.lastname: Hebraud
authors.forename: Ana Cláudia Chagas
authors.initials: AC
authors.lastname: de Paula
authors.forename: Pedro Henrique Leroy
authors.initials: PH
authors.lastname: Viana
authors.forename: Alfredo Miranda
authors.initials: AM
authors.lastname: Goes
authors.forename: Rodrigo L
authors.initials: RL
authors.lastname: Oréfice
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D053687:Adult Stem Cells / Q000166:cytology* / Q000201:enzymology; D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D042783:Endothelial Cells / Q000166:cytology; D005780:Gelatin / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008903:Minerals / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010012:Osteogenesis; D011091:Polyesters / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D008903:Minerals; D011091:Polyesters; C016240:polycaprolactone; D005780:Gelatin; D000469:Alkaline Phosphatase