title: Protective response to subunit vaccination against intranasalBurkholderia malleiandB. pseudomalleichallenge.
abstract: Burkholderia malleiandB. pseudomalleiare Gram-negative pathogenic bacteria, responsible for the diseases glanders and melioidosis, respectively. Furthermore, there is currently no vaccine available against theseBurkholderiaspecies. In this study, we aimed to identify protective proteins against these pathogens. Immunization with recombinantB. malleiHcp1 (type VI secreted/structural protein), BimA (autotransporter protein), BopA (type III secreted protein), andB. pseudomalleiLolC (ABC transporter protein) generated significant protection against lethal inhaledB. malleiATCC23344 andB. pseudomallei1026b challenge. Immunization with BopA elicited the greatest protective activity, resulting in 100% and 60% survival againstB. malleiandB. pseudomalleichallenge, respectively. Moreover, sera from recovered mice demonstrated reactivity with the recombinant proteins. Dendritic cells stimulated with each of the different recombinant proteins showed distinct cytokine patterns. In addition, T cells from immunized mice produced IFN-γ followingin vitrore-stimulation. These results indicated therefore that it was possible to elicit cross-protective immunity against bothB. malleiandB. pseudomalleiby vaccinating animals with one or more novel recombinant proteins identified inB. mallei.
authors.affiliation: Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 77555-1070 ; Department of Clinical Laboratory Sciences, University of Texas Medical Branch, Galveston, Texas 77555-1070.
authors.forename: Gregory C
authors.initials: GC
authors.lastname: Whitlock
authors.affiliation: Department of Pathology, University of Texas Medical Branch, Galveston, Texas 77555-1070.
authors.forename: Arpaporn
authors.initials: A
authors.lastname: Deeraksa
authors.affiliation: Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin Texas 78712.
authors.forename: Omar
authors.initials: O
authors.lastname: Qazi
authors.affiliation: Department of Pathology, University of Texas Medical Branch, Galveston, Texas 77555-1070.
authors.forename: Barbara M
authors.initials: BM
authors.lastname: Judy
authors.affiliation: Department of Pathology, University of Texas Medical Branch, Galveston, Texas 77555-1070.
authors.forename: Katherine
authors.initials: K
authors.lastname: Taylor
authors.affiliation: Department of Microbiology, Immunology and Pathology and Rocky Mountain Regional Center of Excellence Colorado State University, College of Veterinary Medicine, Fort Collins, CO 80523.
authors.forename: Katie L
authors.initials: KL
authors.lastname: Propst
authors.affiliation: Department of Microbiology, Immunology and Pathology and Rocky Mountain Regional Center of Excellence Colorado State University, College of Veterinary Medicine, Fort Collins, CO 80523.
authors.forename: Angie J
authors.initials: AJ
authors.lastname: Duffy
authors.affiliation: Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin Texas 78712.
authors.forename: Kate
authors.initials: K
authors.lastname: Johnson
authors.affiliation: Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin Texas 78712 ; Department of Chemistry and Biochemistry, University of Texas at Austin, Austin Texas 78712.
authors.forename: G Barrie
authors.initials: GB
authors.lastname: Kitto
authors.affiliation: Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin Texas 78712 ; Department of Chemistry and Biochemistry, University of Texas at Austin, Austin Texas 78712 ; Department of Life Sciences, Imperial College London, London, UK SW7 2AZ.
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Brown
authors.affiliation: Department of Microbiology, Immunology and Pathology and Rocky Mountain Regional Center of Excellence Colorado State University, College of Veterinary Medicine, Fort Collins, CO 80523.
authors.forename: Steven W
authors.initials: SW
authors.lastname: Dow
authors.affiliation: Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 77555-1070 ; Department of Pathology, University of Texas Medical Branch, Galveston, Texas 77555-1070 ; The Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas 77555-1070.
authors.forename: Alfredo G
authors.initials: AG
authors.lastname: Torres
authors.affiliation: Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 77555-1070 ; Department of Pathology, University of Texas Medical Branch, Galveston, Texas 77555-1070 ; The Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas 77555-1070.
authors.forename: D Mark
authors.initials: DM
authors.lastname: Estes
keywords: B. mallei; B. pseudomallei; Burkholderia; intranasal infection; subunit vaccination; vaccine