title: Transcriptional analysis of selected cellulose-acting enzymes encoding genes of the white-rot fungus Dichomitus squalens on spruce wood and microcrystalline cellulose.
abstract: The recent discovery of oxidative cellulose degradation enhancing enzymes has considerably changed the traditional concept of hydrolytic cellulose degradation. The relative expression levels of ten cellulose-acting enzyme encoding genes of the white-rot fungus Dichomitus squalens were studied on solid-state spruce wood and in microcrystalline Avicel cellulose cultures. From the cellobiohydrolase encoding genes, cel7c was detected at the highest level and showed constitutive expression whereas variable transcript levels were detected for cel7a, cel7b and cel6 in the course of four-week spruce cultivation. The cellulolytic enzyme activities detected in the liquid cultures were consistent with the transcript levels. Interestingly, the selected lytic polysaccharide monooxygenase (LPMO) encoding genes were expressed in both cultures, but showed different transcription patterns on wood compared to those in submerged microcrystalline cellulose cultures. On spruce wood, higher transcript levels were detected for the lpmos carrying cellulose binding module (CBM) than for the lpmos without CBMs. In both cultures, the expression levels of the lpmo genes were generally higher than the levels of cellobiose dehydrogenase (CDH) encoding genes. Based on the results of this work, the oxidative cellulose cleaving enzymes of D. squalens have essential role in cellulose degrading machinery of the fungus.
authors.affiliation: Department of Food and Environmental Sciences, Division of Microbiology and Biotechnology, University of Helsinki, Viikki Biocenter 1, Finland.
authors.forename: Johanna
authors.initials: J
authors.lastname: Rytioja
authors.affiliation: Department of Food and Environmental Sciences, Division of Microbiology and Biotechnology, University of Helsinki, Viikki Biocenter 1, Finland.
authors.forename: Kristiina
authors.initials: K
authors.lastname: Hildén
authors.affiliation: Department of Food and Environmental Sciences, Division of Microbiology and Biotechnology, University of Helsinki, Viikki Biocenter 1, Finland.
authors.forename: Annele
authors.initials: A
authors.lastname: Hatakka
authors.affiliation: Department of Food and Environmental Sciences, Division of Microbiology and Biotechnology, University of Helsinki, Viikki Biocenter 1, Finland. Electronic address: miia.r.makela@helsinki.fi.
authors.forename: Miia R
authors.initials: MR
authors.lastname: Mäkelä
mesh_terms: D044602:Cellulases / Q000096:biosynthesis* / Q000235:genetics; D002482:Cellulose / Q000378:metabolism*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D028222:Picea / Q000382:microbiology*; D011124:Polyporaceae / Q000201:enzymology* / Q000235:genetics; D017422:Sequence Analysis, DNA; D014934:Wood / Q000378:metabolism* / Q000382:microbiology
chemical_list: D004271:DNA, Fungal; D002482:Cellulose; D044602:Cellulases; C109691:microcrystalline cellulose
keywords: Cellobiohydrolase; Cellobiose dehydrogenase; Dichomitus squalens; Lytic polysaccharide monooxygenase; White-rot fungus