title: Neto auxiliary protein interactions regulate kainate and NMDA receptor subunit localization at mossy fiber-CA3 pyramidal cell synapses.
abstract: Neto1 and Neto2 auxiliary subunits coassemble with NMDA receptors (NMDARs) and kainate receptors (KARs) to modulate their function. In the hippocampus, Neto1 enhances the amplitude and prolongs the kinetics of KAR-mediated currents at mossy fiber (MF)-CA3 pyramidal cell synapses. However, whether Neto1 trafficks KARs to synapses or simply alters channel properties is unresolved. Therefore, postembedding electron microscopy was performed to investigate the localization of GluK2/3 subunits at MF-CA3 synapses in Neto-null mice. Postsynaptic GluK2/3 Immunogold labeling was substantially reduced in Neto-null mice compared with wild types. Moreover, spontaneous KAR-mediated synaptic currents and metabotropic KAR signaling were absent in CA3 pyramidal cells of Neto-null mice. A similar loss of ionotropic and metabotropic KAR function was observed in Neto1, but not Neto2, single knock-out mice, specifically implicating Neto1 in regulating CA3 pyramidal cell KAR localization and function. Additional controversy pertains to the role of Neto proteins in modulating synaptic NMDARs. While Immunogold labeling for GluN2A at MF-CA3 synapses was comparable between wild-type and Neto-null mice, labeling for postsynaptic GluN2B was robustly increased in Neto-null mice. Accordingly, NMDAR-mediated currents at MF-CA3 synapses exhibited increased sensitivity to a GluN2B-selective antagonist in Neto1 knockouts relative to wild types. Thus, despite preservation of the overall MF-CA3 synaptic NMDAR-mediated current, loss of Neto1 alters NMDAR subunit composition. These results confirm that Neto protein interactions regulate synaptic localization of KAR and NMDAR subunits at MF-CA3 synapses, with implications for both ionotropic and metabotropic glutamatergic recruitment of the CA3 network.
authors.affiliation: Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, MSC 3715, Bethesda, Maryland 20892, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, MSC 8027, Bethesda, Maryland 20892, Program in Neurosciences and Mental Health, Hospital for Sick Children, and Department of Physiology, University of Toronto, Toronto, Ontario M5G 1X8, Canada, and Lady Davis Research Institute, Jewish General Hospital and Departments of Human Genetics and Biochemistry, McGill University, Montréal, Québec H3T 1E2, Canada.
authors.forename: Megan S
authors.initials: MS
authors.lastname: Wyeth
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Pelkey
authors.forename: Ronald S
authors.initials: RS
authors.lastname: Petralia
authors.forename: Michael W
authors.initials: MW
authors.lastname: Salter
authors.forename: Roderick R
authors.initials: RR
authors.lastname: McInnes
authors.forename: Chris J
authors.initials: CJ
authors.lastname: McBain
mesh_terms: D000818:Animals; D056654:CA3 Region, Hippocampal / Q000378:metabolism*; D026502:LDL-Receptor Related Proteins; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D019599:Mossy Fibers, Hippocampal / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D018092:Kainic Acid Receptors / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism
chemical_list: D026502:LDL-Receptor Related Proteins; D008077:Lipoproteins, LDL; D008565:Membrane Proteins; D018092:Kainic Acid Receptors; D016194:Receptors, N-Methyl-D-Aspartate; C539204:NETO2 protein, mouse; C486748:Neto1 protein, mouse
keywords: NMDAR; Neto1 or Neto2; electron microscopy; kainate receptor; metabotropic; mossy fiber pathway