title: Proteomic analysis of proton beam irradiated human melanoma cells.
abstract: Proton beam irradiation is a form of advanced radiotherapy providing superior distributions of a low LET radiation dose relative to that of photon therapy for the treatment of cancer. Even though this clinical treatment has been developing for several decades, the proton radiobiology critical to the optimization of proton radiotherapy is far from being understood. Proteomic changes were analyzed in human melanoma cells treated with a sublethal dose (3 Gy) of proton beam irradiation. The results were compared with untreated cells. Two-dimensional electrophoresis was performed with mass spectrometry to identify the proteins. At the dose of 3 Gy a minimal slowdown in proliferation rate was seen, as well as some DNA damage. After allowing time for damage repair, the proteomic analysis was performed. In total 17 protein levels were found to significantly (more than 1.5 times) change: 4 downregulated and 13 upregulated. Functionally, they represent four categories: (i) DNA repair and RNA regulation (VCP, MVP, STRAP, FAB-2, Lamine A/C, GAPDH), (ii) cell survival and stress response (STRAP, MCM7, Annexin 7, MVP, Caprin-1, PDCD6, VCP, HSP70), (iii) cell metabolism (TIM, GAPDH, VCP), and (iv) cytoskeleton and motility (Moesin, Actinin 4, FAB-2, Vimentin, Annexin 7, Lamine A/C, Lamine B). A substantial decrease (2.3 x) was seen in the level of vimentin, a marker of epithelial to mesenchymal transition and the metastatic properties of melanoma.
authors.affiliation: Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland ; Malopolska Centre of Biotechnology, Krakow, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Kedracka-Krok
authors.affiliation: Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland ; Malopolska Centre of Biotechnology, Krakow, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Jankowska
authors.affiliation: Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.
authors.forename: Martyna
authors.initials: M
authors.lastname: Elas
authors.affiliation: Institute of Nuclear Physics, PAS, Kraków, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Sowa
authors.affiliation: Institute of Nuclear Physics, PAS, Kraków, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Swakon
authors.affiliation: Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Cierniak
authors.affiliation: Institute of Nuclear Physics, PAS, Kraków, Poland.
authors.forename: Pawel
authors.initials: P
authors.lastname: Olko
authors.affiliation: Department of Ophthalmology and Ophthalmic Oncology, Jagiellonian University Medical College, Kraków, Poland.
authors.forename: Bozena
authors.initials: B
authors.lastname: Romanowska-Dixon
authors.affiliation: Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Urbanska
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D004249:DNA Damage / Q000528:radiation effects; D006801:Humans; D008545:Melanoma / Q000378:metabolism* / Q000532:radiotherapy; D020543:Proteome; D040901:Proteomics / Q000379:methods; D011522:Protons; D013312:Stress, Physiological
chemical_list: D020543:Proteome; D011522:Protons