title: Characterization of Mannheimia haemolytica isolated from feedlot cattle that were healthy or treated for bovine respiratory disease.
abstract: Mannheimia haemolytica is the principal bacterial pathogen associated with bovine respiratory disease (BRD). As an opportunistic pathogen, M. haemolytica is also frequently isolated from the respiratory tract of healthy cattle. This study examined the characteristics of M. haemolytica collected using deep nasal swabs from healthy cattle (n = 49) and cattle diagnosed with BRD (n = 41). Isolates were analyzed by pulsed-field gel electrophoresis (PFGE), serotyped, and tested for antimicrobial susceptibility. Polymerase chain reaction (PCR) was used to screen isolates for virulence [leukotoxin C (lktC), putative adhesin (ahs), outer-membrane lipoprotein (gs60), O-sialoglycoprotease (gcp), transferring-binding protein B (tbpB) and UDP-N-acetyl-D-glucosamine-2-epimerase (nmaA)] and antimicrobial resistance [tet(H), bla ROB-1, erm(X), erm(42), msr(E)-mph(E) and aphA-1] genes. Isolates were genetically diverse but in three instances, M. haemolytica with the same pulsotype, resistance phenotype, and genotype were collected from cattle with BRD. This occurred once between cattle located in two different feedlots, once between cattle in the same feedlot, but in different pens, and once among cattle from the same feedlot in the same pen. Isolates from healthy cattle were primarily serotype 2 (75.5%) while those from individuals with BRD were serotype 1 (70.7%) or 6 (19.5%). Resistance to at least one antibiotic occurred more frequently (P < 0.001) in M. haemolytica collected from cattle with BRD (37%) compared with those that were healthy (2%). Overall, tetracycline resistance (18%) was the most prevalent resistant phenotype. All tetracycline-resistant M. haemolytica encoded tet(H). Ampicillin resistance (6%) and neomycin resistance (15%) were detected and corresponded to the presence of the bla ROB-1 and aphA-1 genes, respectively. Tilmicosin resistance (6%) was also detected, but the resistance genes responsible were not identified. The virulence genes lktC, ahs, gs60, and gcp were present in all isolates examined, while tbpB and nmaA were only detected in serotype 1 and serotype 6 isolates indicating they may be potential targets for serotype-specific identification or vaccine development. These results provide the first reported evidence of transmission and spread of antimicrobial-resistant M. haemolytica that have contributed to bovine respiratory disease in western Canadian feedlots.
authors.affiliation: Agriculture and Agri-Food Canada Research Centre, Lethbridge, Alberta T1J 4B1, Canada (Klima, Alexander, McAllister); Department of Large Animal Clinial Science, Western College of Veterinary Medicine, University of Saskatoon, Saskatoon, Saskatchewan S7N 5B4, Canada (Klima, Hendrick).
authors.forename: Cassidy L
authors.initials: CL
authors.lastname: Klima
authors.affiliation: Agriculture and Agri-Food Canada Research Centre, Lethbridge, Alberta T1J 4B1, Canada (Klima, Alexander, McAllister); Department of Large Animal Clinial Science, Western College of Veterinary Medicine, University of Saskatoon, Saskatoon, Saskatchewan S7N 5B4, Canada (Klima, Hendrick).
authors.forename: Trevor W
authors.initials: TW
authors.lastname: Alexander
authors.affiliation: Agriculture and Agri-Food Canada Research Centre, Lethbridge, Alberta T1J 4B1, Canada (Klima, Alexander, McAllister); Department of Large Animal Clinial Science, Western College of Veterinary Medicine, University of Saskatoon, Saskatoon, Saskatchewan S7N 5B4, Canada (Klima, Hendrick).
authors.forename: Steve
authors.initials: S
authors.lastname: Hendrick
authors.affiliation: Agriculture and Agri-Food Canada Research Centre, Lethbridge, Alberta T1J 4B1, Canada (Klima, Alexander, McAllister); Department of Large Animal Clinial Science, Western College of Veterinary Medicine, University of Saskatoon, Saskatoon, Saskatchewan S7N 5B4, Canada (Klima, Hendrick).
authors.forename: Tim A
authors.initials: TA
authors.lastname: McAllister
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques; D048090:Bovine Respiratory Disease Complex / Q000382:microbiology*; D002417:Cattle; D024881:Drug Resistance, Bacterial; D005260:Female; D016978:Mannheimia haemolytica / Q000145:classification* / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests; D009305:Nasopharynx / Q000382:microbiology; D016871:Pasteurellaceae Infections / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012703:Serotyping
chemical_list: D000900:Anti-Bacterial Agents