title: Sprouty4 interferes with cell proliferation and migration of breast cancer-derived cell lines.
abstract: Sprouty proteins are modulators of mitogen-induced signal transduction processes and therefore can influence the process of cancerogenesis. In particular, Sprouty2 has been shown to have an important role in cancer development of many tumor entities including breast cancer. In this report, we investigated the role of Sprouty4 in breast cancer-derived cell lines. We have found that ectopic Sprouty4 expression inhibits cell proliferation of breast cancer cell lines independently of their endogenous expression levels. Corroborating Sprouty4 downregulation causes accelerated growth. Furthermore, we demonstrate that an increase in Sprouty4 content interferes with serum-induced activation of mitogen-activated protein kinase pathway. Additionally, Sprouty4 expression negatively influences cell migration. These data suggest that Sprouty4 is a possible candidate for a tumor suppressor in breast cancer.
authors.affiliation: Institute of Cancer Research, Department of Medicine I, Medical University of Vienna, Borschkegasse 8a, 1090, Vienna, Austria.
authors.forename: Vanita
authors.initials: V
authors.lastname: Vanas
authors.forename: Elsa
authors.initials: E
authors.lastname: Mühlbacher
authors.forename: Rosana
authors.initials: R
authors.lastname: Kral
authors.forename: Hedwig
authors.initials: H
authors.lastname: Sutterlüty-Fall
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000502:physiology; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis / Q000502:physiology*; D008565:Membrane Proteins / Q000032:analysis / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000502:physiology*; D025521:Tumor Suppressor Proteins / Q000502:physiology
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C485561:SPRY2 protein, human; C499038:SPRY4 protein, human; D025521:Tumor Suppressor Proteins; D048049:Extracellular Signal-Regulated MAP Kinases