title: Kinetics of nutrient removal and expression of extracellular polymeric substances of the microalgae, Chlorella sp. and Micractinium sp., in wastewater treatment.
abstract: Two species of green algae, Chlorella sp. and Micractinium sp., were cultivated in primary effluent wastewater and high-strength wastewater (a mixture of anaerobic digestion centrate and primary effluent) to study nutrient removal and EPS (extracellular polymeric substances) expression during their growth. The high N concentration and P-limited condition in the mixed wastewater (total N=197 mg/L; N/P mass ratio=56) led to about 3 times greater specific N removal rate than the primary effluent set, indicating that algal cells growing in N-rich wastewater had N over-uptake. Both Chlorella and Micractinium grown in the high-strength wastewater also produced larger amounts of protein EPS, possibly accounting for higher N uptake in those cultivation sets. These results suggest that different types of wastewater could cause different nutrient removal kinetics and EPS expression by algae, which may subsequently influence harvesting and anaerobic digestion of their biomass.
authors.affiliation: Department of Civil and Environmental Engineering, University of Massachusetts Amherst, United States.
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Civil and Environmental Engineering, University of Massachusetts Amherst, United States.
authors.forename: Wenye Camilla
authors.initials: WC
authors.lastname: Kuo-Dahab
authors.affiliation: Department of Civil and Environmental Engineering, University of Massachusetts Amherst, United States.
authors.forename: Sona
authors.initials: S
authors.lastname: Dolan
authors.affiliation: Department of Civil and Environmental Engineering, University of Massachusetts Amherst, United States. Electronic address: park@ecs.umass.edu.
authors.forename: Chul
authors.initials: C
authors.lastname: Park
mesh_terms: D001673:Biodegradation, Environmental; D057919:Biological Oxygen Demand Analysis; D001704:Biopolymers / Q000378:metabolism*; D002412:Cations / Q000302:isolation & purification; D002708:Chlorella / Q000254:growth & development / Q000378:metabolism*; D005110:Extracellular Space / Q000737:chemistry; D007700:Kinetics; D058086:Microalgae / Q000254:growth & development / Q000378:metabolism*; D009584:Nitrogen / Q000302:isolation & purification*; D010758:Phosphorus / Q000302:isolation & purification*; D012995:Solubility; D013010:Sonication; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification; D018508:Water Purification / Q000379:methods*
chemical_list: D001704:Biopolymers; D002412:Cations; D009584:Nitrogen; D010758:Phosphorus; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Extracellular polymeric substances (EPS); Kinetics; Microalgae; Nutrient removal; Wastewater