title: [Role of Dlx1 natural antisense transcript in mice brain development].
abstract: OBJECTIVE
  To explore the role of Dlx1as, a natural antisense transcript, in mice brain development.
  
  
  METHODS
  Based on the bioinformatic and molecular biological findings, we confirmed whether the Dlx1as has a poly-A tail. The temporal expressions of Dlx1as and Dlx1 mRNA were determined by quantitative real-time PCR and the spatial expressions of Dlx1as and Dlx1 mRNA were compared by using in situ hybridization.
  
  
  RESULTS
  Dlx1as had a poly-A signal. Dlx1as's expression was at a high level through a period of time in the embryo development, which was similar with Dlx1 mRNA in temporal but different in spatial.
  
  
  CONCLUSIONS
  Dlx1as is highly expressed in a certain period during mice brain development, which spatially supplements the mRNA expression of D1x1. Therefore, it is supposed that Dlx1as influences the mice brain development by regulating Dlx1 mRNA.
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: A-ni
authors.initials: AN
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Li-yuan
authors.initials: LY
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001921:Brain / Q000254:growth & development* / Q000378:metabolism; D018398:Homeodomain Proteins / Q000378:metabolism*; D017403:In Situ Hybridization; D051379:Mice; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: C088334:Distal-less homeobox proteins; D018398:Homeodomain Proteins; D012333:RNA, Messenger; D014157:Transcription Factors