title: Molecular cloning and expression analysis of two lipopolysaccharide-induced TNF-α factors (LITAFs) from rock bream, Oplegnathus fasciatus.
abstract: Lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-α factor (LITAF) plays an important role controlling the expression of TNF-α and the other cytokine genes in the presence of LPS. However, two LITAF homologues have not been characterized in fish. In this study, we cloned two distinct LITAF (RbLITAF1 and RbLITAF2) cDNAs from rock bream (Oplegnathus fasciatus) and characterized their expression profiles after infection with Edwardsiella tarda, Streptococcus iniae or red seabream iridovirus (RSIV). The coding regions of RbLITAF1 and RbLITAF2 cDNAs were 492 bp and 417 bp, encoding 153 and 138 amino acid residues, respectively. The genes consisted of a LITAF domain. RbLITAF1 was highly expressed in the spleen and heart of healthy rock bream, whereas RbLITAF2 was highly expressed in the gill, intestine and stomach. In spleen, the gene expression of RbLITAF1 and RbLITAF2 were increased until 5 days post-infection (dpi), and then decreased at 7 dpi. In kidney, E. tarda and RSIV infection led to induction of the RbLITAF1 gene at 1 dpi, RbLITAF2 gene was down-regulated after pathogen infection. These results suggest that RbLITAFs may be involved in the LITAF-mediated immune response and regulate systemic immune responses against pathogen infection.
authors.affiliation: Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea.
authors.forename: Seong Don
authors.initials: SD
authors.lastname: Hwang
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea.
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Shim
authors.affiliation: Pathology Division, National Fisheries Research and Development Institute, Busan 619-900, Republic of Korea.
authors.forename: Mun-Gyeong
authors.initials: MG
authors.lastname: Kwon
authors.affiliation: Oil & POPs Research Group, Korea Institute of Ocean Science & Technology, 391 Jangbuk-Ri, Jangmok-Myon, Geoje 656-834, Republic of Korea.
authors.forename: Young Sun
authors.initials: YS
authors.lastname: Chae
authors.affiliation: Oil & POPs Research Group, Korea Institute of Ocean Science & Technology, 391 Jangbuk-Ri, Jangmok-Myon, Geoje 656-834, Republic of Korea.
authors.forename: Won Joon
authors.initials: WJ
authors.lastname: Shim
authors.affiliation: Oil & POPs Research Group, Korea Institute of Ocean Science & Technology, 391 Jangbuk-Ri, Jangmok-Myon, Geoje 656-834, Republic of Korea.
authors.forename: Jee-Hyun
authors.initials: JH
authors.lastname: Jung
authors.affiliation: Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea.
authors.forename: Ju-Won
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea. Electronic address: vinus96@hanmail.net.
authors.forename: Chan-Il
authors.initials: CI
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D020609:Edwardsiella tarda / Q000502:physiology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D007113:Immunity, Innate / Q000187:drug effects; D007497:Iridoviridae / Q000502:physiology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D010473:Perciformes / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D010802:Phylogeny; D012313:RNA / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary; D013291:Streptococcus / Q000502:physiology
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D008070:Lipopolysaccharides; D012313:RNA
keywords: Edwardsiella tarda; LITAF; Oplegnathus fasciatus; Red seabream iridovirus; Streptococcus iniae