title: Comparison of an antigen-capture enzyme-linked immunosorbent assay with bacterial culture for detection of Salmonella in poultry-hatchery environmental samples.
abstract: An antigen-capture, monoclonal-antibody-based enzyme-linked immunoassay (ELISA) that detects a broad range of Salmonella serovars in various serogroups was developed and compared with standard culture procedures for detection of Salmonella in 1055 field samples collected from poultry-hatchery environments. The diagnostic sensitivity of the ELISA relative to culture was 99.9% and the diagnostic specificity 99.6%. The extensive culture procedure included nonselective enrichment (NSE) as well as primary selective enrichment (PSE) and delayed secondary enrichment (DSE) with Hajna tetrathionate (TT) and Rappaport-Vassiliadis (RV) selective-enrichment broths. Significantly more Salmonella-positive samples were detected by ELISA and culture at the DSE stage than at the NSE and PSE stages (P < 0.05). Significantly more RV than TT broths were positive for Salmonella by culture and ELISA by the DSE stage (P < 0.05). This ELISA procedure could be a reliable screening test for the detection of Salmonella in hatchery samples.
authors.affiliation: Canadian Food Inspection Agency, Ottawa Laboratory (Fallowfield), 3851 Fallowfield Road, PO Box 11300, Station H, Ottawa, Ontario K2H 8P9.
authors.forename: Brian W
authors.initials: BW
authors.lastname: Brooks
authors.affiliation: Canadian Food Inspection Agency, Ottawa Laboratory (Fallowfield), 3851 Fallowfield Road, PO Box 11300, Station H, Ottawa, Ontario K2H 8P9.
authors.forename: Cheryl L
authors.initials: CL
authors.lastname: Lutze-Wallace
authors.affiliation: Canadian Food Inspection Agency, Ottawa Laboratory (Fallowfield), 3851 Fallowfield Road, PO Box 11300, Station H, Ottawa, Ontario K2H 8P9.
authors.forename: John
authors.initials: J
authors.lastname: Devenish
authors.affiliation: Canadian Food Inspection Agency, Ottawa Laboratory (Fallowfield), 3851 Fallowfield Road, PO Box 11300, Station H, Ottawa, Ontario K2H 8P9.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Elmufti
authors.affiliation: Canadian Food Inspection Agency, Ottawa Laboratory (Fallowfield), 3851 Fallowfield Road, PO Box 11300, Station H, Ottawa, Ontario K2H 8P9.
authors.forename: Teresa
authors.initials: T
authors.lastname: Burke
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry*; D004783:Environmental Microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D006799:Housing, Animal; D011200:Poultry; D012475:Salmonella / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D000942:Antigens, Bacterial