title: Alteration of mitochondrial function and ultrastructure in the hippocampus of pilocarpine-treated rat.
abstract: Present studies were carried out to decipher seizure-dependent changes in mitochondrial function and ultrastructure in rat hippocampus after status epilepticus (SE) induced by pilocarpine (PILO). Discernible mitochondrial ultrastructural damage was observed in the hippocampus. Enzyme assay revealed cytochrome oxidase (COX) activity significantly increased 3h after SE, decreased 7 d and 45 d after SE, whereas succinate dehydrogenase (SDH) activity displayed no significant changes. Quantitative real-time PCR and Western blotting showed that COX III (mitochondrial-encoded) mRNA and protein level were significantly increased at 3h, decreased to the control level on 7d and dropped significantly on 45 d; the corresponding expression of COX IV were not changed by PILO at any time tested. The results were also supported by immunohistochemistry. Thus, our results demonstrate that dysfunction of mitochondrial complex IV respiratory enzyme and mitochondrial ultrastructural damage in the hippocampus are associated with prolonged seizure during experimental temporal lobe epilepsy and mitochondria are more vulnerable to epileptic damage.
authors.affiliation: Department of Neurology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao 266000, China. Electronic address: jinggao0611@aliyun.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Geriatric Neurology, Provincial Hospital Affiliated to Shandong University, Jinan 250012, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yao
authors.affiliation: Department of Neurology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao 266000, China.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Pan
authors.affiliation: Department of Neurology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao 266000, China.
authors.forename: An-Mu
authors.initials: AM
authors.lastname: Xie
authors.affiliation: Department of Neurology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao 266000, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Neurology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao 266000, China.
authors.forename: Jing-Hui
authors.initials: JH
authors.lastname: Song
authors.affiliation: Department of Neurology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao 266000, China.
authors.forename: Ai-Jun
authors.initials: AJ
authors.lastname: Ma
authors.affiliation: Department of Neurology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao 266000, China.
authors.forename: Zong-Chao
authors.initials: ZC
authors.lastname: Liu
mesh_terms: D000818:Animals; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology* / Q000502:physiology; D008297:Male; D008928:Mitochondria / Q000473:pathology* / Q000502:physiology / Q000648:ultrastructure*; D010862:Pilocarpine / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013226:Status Epilepticus / Q000139:chemically induced / Q000473:pathology* / Q000503:physiopathology*
chemical_list: D010862:Pilocarpine
keywords: Cytochrome oxidase; Epilepsy; Hippocampus; Mitochondria; Status epilepticus and seizures; Succinate dehydrogenase