title: Quantitative proteomics and protein network analysis of A549 lung cancer cells affected by miR-206.
abstract: MiR-206 acts as a potential tumor suppressor during carcinogenesis and a regulatory factor in osteoblasts differentiation, but its modulatory mechanism remains unclear. In this study, we used a quantitative proteomics method, difference gel electrophoresis (DIGE), to profile the protein variation in A549 lung cancer cells with and without miR- 206 transfection. We identified a total of 17 differently expressed proteins including 5 up-regulated and 12 down-regulated proteins affected by miR-206 in A549 cells. We further constructed a protein network linked 17 differently expressed proteins with 106 computationally predicted miR-206 targets, and identified 8 "hub" genes (CALR, CTSD, ENO1, HSPA5, CDC42, HSPD1, POLA1, and SMARCA4) within the network, which may represent important miR-206 functional gene targets. In conclusion, in this study, we identified several candidate functional target genes for miR-206, which is helpful to further explore its mechanisms during carcinogenesis and osteogenesis, and we also proposed a novel proteomic strategy to identify functionally important gene targets for microRNA.
authors.affiliation: Shandong Medical Biotechnological Center, Shandong Academy of Medical Sciences, Key Laboratory for Biotech Drugs of the Ministry of Health, Ji'nan, Shandong, China.
authors.forename: Yazhou
authors.initials: Y
authors.lastname: Cui
authors.forename: Shuyang
authors.initials: S
authors.lastname: Xie
authors.forename: Jing
authors.initials: J
authors.lastname: Luan
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhou
authors.forename: Jinxiang
authors.initials: J
authors.lastname: Han
mesh_terms: D045744:Cell Line, Tumor; D019295:Computational Biology; D000091342:Endoplasmic Reticulum Chaperone BiP; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; C546087:MIRN206 microRNA, human; D035683:MicroRNAs