title: Quencher-free molecular beacons as probes for oligonucleotides containing CAG repeat sequences.
abstract: We have identified quencher-free molecular beacons that allow the sensitive probing of CAG repeat oligonucleotides, including mRNA fragments of trinucleotide repeat diseases, with significant increases in fluorescence intensity mediated by disruption of the stacking of their (Py)U units.
authors.affiliation: Department of Chemistry, BK School of Molecular Science, Pohang University of Science and Technology, Pohang 790-784, South Korea. bhkim@postech.ac.kr.
authors.forename: Ki Tae
authors.initials: KT
authors.lastname: Kim
authors.forename: Rakesh N
authors.initials: RN
authors.lastname: Veedu
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Seo
authors.forename: Byeang Hyean
authors.initials: BH
authors.lastname: Kim
mesh_terms: D004247:DNA / Q000737:chemistry; D005453:Fluorescence; D006801:Humans; D015336:Molecular Probe Techniques; D015341:Nucleic Acid Probes / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D012333:RNA, Messenger / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D018911:Trinucleotide Repeats
chemical_list: D015341:Nucleic Acid Probes; D009841:Oligonucleotides; D012333:RNA, Messenger; D004247:DNA