title: Effect of Human Endothelial Progenitor Cell (EPC)- or Mouse Vascular Endothelial Growth Factor-Derived Vessel Formation on the Survival of Vitrified/Warmed Mouse Ovarian Grafts.
abstract: The aim of this study was to evaluate the effectiveness of improving angiogenesis at graft sites on the survival of follicles in transplanted ovarian tissue. Matrigel containing 5 × 105of cord blood-derived endothelial progenitor cells (EPCs) or 200 ng of mouse vascular endothelial growth factor (VEGF) was injected subcutaneously into BALB/c-Nu mice. After 1 week, vitrified/warmed ovaries from female B6D2F1 mice were subcutaneously transplanted into the injection sites. After 1, 2, and 4 weeks posttransplantation, the ovaries were recovered and subjected to histological analysis. Oocytes were collected from the transplanted ovaries, and their fertilization, embryonic development, and delivery were also observed. Vitrified/warmed ovaries transplanted into EPC- or VEGF-treated sites developed more blood vessels and showed better follicle survival than those transplanted into sham-injected sites. Normal embryonic development and consequent live births were obtained using oocytes recovered from cryopreserved/transplanted ovaries. Treatment with EPCs or VEGF could prevent the ischemic damage during the early revascularization stage of ovarian transplantation.
authors.affiliation: Fertility Center of CHA Gangnam Medical Center, College of Medicine, CHA University, Seoul, Korea Department of Biomedical Science, College of Life Science, CHA University, Seoul, Korea.
authors.forename: Soo Kyung
authors.initials: SK
authors.lastname: Cha
authors.affiliation: Fertility Center of CHA Gangnam Medical Center, College of Medicine, CHA University, Seoul, Korea.
authors.forename: Dong Hyuk
authors.initials: DH
authors.lastname: Shin
authors.affiliation: Fertility Center of CHA Gangnam Medical Center, College of Medicine, CHA University, Seoul, Korea.
authors.forename: Bo Yeun
authors.initials: BY
authors.lastname: Kim
authors.affiliation: Fertility Center of CHA Gangnam Medical Center, College of Medicine, CHA University, Seoul, Korea Department of Biomedical Science, College of Life Science, CHA University, Seoul, Korea.
authors.forename: Sook-Young
authors.initials: SY
authors.lastname: Yoon
authors.affiliation: Fertility Center of CHA Gangnam Medical Center, College of Medicine, CHA University, Seoul, Korea.
authors.forename: Tae Ki
authors.initials: TK
authors.lastname: Yoon
authors.affiliation: Fertility Center of CHA Gangnam Medical Center, College of Medicine, CHA University, Seoul, Korea.
authors.forename: Woo Sik
authors.initials: WS
authors.lastname: Lee
authors.affiliation: Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, Korea drleedr@cha.ac.kr hmchung@kku.ac.kr.
authors.forename: Hyung Min
authors.initials: HM
authors.lastname: Chung
authors.affiliation: Fertility Center of CHA Gangnam Medical Center, College of Medicine, CHA University, Seoul, Korea Department of Biomedical Science, College of Life Science, CHA University, Seoul, Korea.
authors.forename: Dong Ryul
authors.initials: DR
authors.lastname: Lee
keywords: blood vessel formation; endothelial progenitor cells; follicle survival; heterotopic transplantation; ovary cryopreservation