title: [Correlation study of HPV-16 existential status with Th17/Treg cytokines].
abstract: OBJECTIVE
  To explore the relationship between the presence of HPV-16 DNA and the expression Treg surface marker Foxp3(+), peripheral blood levels of Th17/Treg cell-associated cytokines and explore their roles and significance in cervical cancer progression.
  
  
  METHODS
  Between January 2012 and October 2012 at Shengjing Hospital of China Medical University, a total of 142 HPV16 positive patients were divided into cervical cancer (CC, n = 60), cervical intraepithelial neoplasia (CIN, n = 65) and control group (n = 17). Cervical liquid-based cytological (LBC) samples were collected to detect E2 and E6 genes of HPV type 16 using multiple real-time polymerase chain reaction (PCR). E2/E6 ratio was used to evaluate the physical status of HPV-16 DNA in host cell genome. The SP immunohistochemical method was used to detect the expressions of FOXP3 in cervical lesions. The concentrations of Th17/Treg cell-associated cytokines were detected by enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  Under the same status of HPV16 DNA in vivo, the levels of Foxp3(+), transforming growth factor-β (TGF-β) and interleukin-10 (IL-10) were significantly higher than those of the control group (P < 0.01) while the levels of interleukin-17 (IL-17) and interleukin-21 (IL-21)were significantly lower than those of the control group (P < 0.05) . In the same disease, HPV16 DNA integration rate grew with the increases of Foxp3(+), TGF-β and IL-10 while IL-17 and IL-21 were opposite. In the different status of HPV16 type DNA, the expression of Foxp3(+) was closely correlated with Federation International of Gynecology and Obstetrics (FIGO) stage, histological grade and lymphnode metastasis (P < 0.05) except for age (P > 0.05).
  
  
  CONCLUSIONS
  Treg cytokines, HPV16 integration rate and severity of cervical lesions are positively correlated while Th17 cytokines show opposite effects. Th17/Treg cell-associated cytokines may play an important role in the occurrence and development of cervical cancer.
authors.affiliation: Department of Obstetrics & Gynecology, Affiliated Shengjing Hospital, China Medical University, Shenyang 110004, China.
authors.forename: Ai-ping
authors.initials: AP
authors.lastname: Ding
authors.affiliation: Department of Obstetrics & Gynecology, Affiliated Shengjing Hospital, China Medical University, Shenyang 110004, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics & Gynecology, Affiliated Shengjing Hospital, China Medical University, Shenyang 110004, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Wei
authors.affiliation: Department of Obstetrics & Gynecology, Affiliated Shengjing Hospital, China Medical University, Shenyang 110004, China.
authors.forename: Qing-shuang
authors.initials: QS
authors.lastname: Luo
authors.affiliation: Department of Obstetrics & Gynecology, Affiliated Shengjing Hospital, China Medical University, Shenyang 110004, China. Email: zsl0909@sina.com.
authors.forename: Shu-lan
authors.initials: SL
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D016207:Cytokines / Q000276:immunology*; D004279:DNA, Viral / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000032:analysis; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D052162:Human papillomavirus 16 / Q000502:physiology; D006801:Humans; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000032:analysis; D012097:Repressor Proteins / Q000032:analysis; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism / Q000821:virology; D058504:Th17 Cells / Q000378:metabolism / Q000821:virology; D002583:Uterine Cervical Neoplasms / Q000276:immunology* / Q000378:metabolism / Q000821:virology*; D016662:Virus Integration; D002578:Uterine Cervical Dysplasia / Q000821:virology
chemical_list: D016207:Cytokines; D004279:DNA, Viral; D004268:DNA-Binding Proteins; C054855:E2 protein, Human papillomavirus type 16; C068651:E6 protein, Human papillomavirus type 16; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins