title: Immunobiotic lactobacilli reduce viral-associated pulmonary damage through the modulation of inflammation-coagulation interactions.
abstract: The exacerbated disease due to immune- and coagulative-mediated pulmonary injury during acute respiratory viruses infection results in severe morbidity and mortality. Identifying novel approaches to modulate virus-induced inflammation-coagulation interactions could be important alternatives for treating acute respiratory viruses infections. In this study we investigated the effect of the probiotic strain Lactobacillus rhamnosus CRL1505 on lung TLR3-mediated inflammation, and its ability to modulate inflammation-coagulation interaction during respiratory viral infection. Our findings reveal for the first time that a probiotic bacterium is able to influence lung immune-coagulative reaction triggered by TLR3 activation, by modulating the production of proinflammatory and anti-inflammatory cytokines as well as expression of tissue factor and thrombomodulin in the lung. We also demonstrated that the preventive treatment with the probiotic bacteria beneficially modulates the fine tune balance between clearing respiratory viruses (respiratory syncytial virus and influenza virus) and controlling immune-coagulative responses in the lung, allowing normal lung function to be maintained in the face of a viral attack. Our data also pinpoint a crucial role for IL-10 in the immune protection induced by L. rhamnosus CRL1505 during respiratory viral infections. These observations might be helpful to propose new preventive or therapeutic approaches to better control virus-inflammatory lung damage using probiotic functional foods.
authors.affiliation: Immunobiotics Research Group; Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman, Argentina; Applied Biochemistry Institute, Faculty of Biochemistry, Chemistry and Pharmacy, Tucuman University, Tucuman, Argentina.
authors.forename: Hortensia
authors.initials: H
authors.lastname: Zelaya
authors.affiliation: Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Kohichiro
authors.initials: K
authors.lastname: Tsukida
authors.affiliation: Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Chiba
authors.affiliation: Immunobiotics Research Group; Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman, Argentina.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Marranzino
authors.affiliation: Immunobiotics Research Group; Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman, Argentina; Applied Biochemistry Institute, Faculty of Biochemistry, Chemistry and Pharmacy, Tucuman University, Tucuman, Argentina.
authors.forename: Susana
authors.initials: S
authors.lastname: Alvarez
authors.affiliation: Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Haruki
authors.initials: H
authors.lastname: Kitazawa
authors.affiliation: Immunobiotics Research Group; Applied Biochemistry Institute, Faculty of Biochemistry, Chemistry and Pharmacy, Tucuman University, Tucuman, Argentina. Electronic address: gaguero@fbqf.unt.edu.ar.
authors.forename: Graciela
authors.initials: G
authors.lastname: Agüero
authors.affiliation: Immunobiotics Research Group; Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman, Argentina; Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. Electronic address: jcvillena@cerela.org.ar.
authors.forename: Julio
authors.initials: J
authors.lastname: Villena
mesh_terms: D000818:Animals; D000990:Antithrombin III / Q000276:immunology; D001777:Blood Coagulation / Q000187:drug effects*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002522:Chlorocebus aethiops; D016207:Cytokines / Q000276:immunology; D004285:Dogs; D007155:Immunologic Factors / Q000494:pharmacology*; D029521:Alphainfluenzavirus; D052201:Lacticaseibacillus rhamnosus; D007958:Leukocyte Count; D061985:Madin Darby Canine Kidney Cells; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000097:blood / Q000276:immunology / Q000821:virology; D010447:Peptide Hydrolases / Q000276:immunology; D011014:Pneumonia / Q000097:blood / Q000139:chemically induced / Q000276:immunology; D011070:Poly I-C; D019936:Probiotics / Q000494:pharmacology*; D018357:Respiratory Syncytial Virus Infections / Q000097:blood / Q000276:immunology / Q000821:virology; D018113:Respiratory Syncytial Virus, Human; D014709:Vero Cells
chemical_list: D016207:Cytokines; D007155:Immunologic Factors; C046193:antithrombin III-protease complex; D000990:Antithrombin III; D010447:Peptide Hydrolases; D011070:Poly I-C
keywords: IL-10; Inflammation–Coagulation; Influenza virus; Lactobacillus rhamnosus CRL1505; Poly(I:C); Respiratory syncytial virus; TLR3