title: Real-time monitoring of 3T3-L1 preadipocyte differentiation using a commercially available electric cell-substrate impedance sensor system.
abstract: Real-time analysis offers multiple benefits over traditional end point assays. Here, we present a method of monitoring the optimisation of the growth and differentiation of murine 3T3-L1 preadipocytes to adipocytes using the commercially available ACEA xCELLigence Real-Time Cell Analyser Single Plate (RTCA SP) system. Our findings indicate that the ACEA xCELLigence RTCA SP can reproducibly monitor the primary morphological changes in pre- and post-confluent 3T3-L1 fibroblasts induced to differentiate using insulin, dexamethasone, 3-isobutyl-1-methylxanthine and rosiglitazone; and may be a viable primary method of screening compounds for adipogenic factors.
authors.affiliation: Biomedical Biotechnology Research Unit, Department of Biochemistry, Microbiology and Biotechnology, PO Box 94, Rhodes University, Grahamstown 6140, South Africa.
authors.forename: Adam H
authors.initials: AH
authors.lastname: Kramer
authors.affiliation: Biomedical Biotechnology Research Unit, Department of Biochemistry, Microbiology and Biotechnology, PO Box 94, Rhodes University, Grahamstown 6140, South Africa.
authors.forename: Julia
authors.initials: J
authors.lastname: Joos-Vandewalle
authors.affiliation: Biomedical Biotechnology Research Unit, Department of Biochemistry, Microbiology and Biotechnology, PO Box 94, Rhodes University, Grahamstown 6140, South Africa.
authors.forename: Adrienne L
authors.initials: AL
authors.lastname: Edkins
authors.affiliation: Department of Biochemistry and Microbiology, PO Box 77000, Nelson Mandela Metropolitan University, Port Elizabeth 6031, South Africa.
authors.forename: Carminita L
authors.initials: CL
authors.lastname: Frost
authors.affiliation: Biomedical Biotechnology Research Unit, Department of Biochemistry, Microbiology and Biotechnology, PO Box 94, Rhodes University, Grahamstown 6140, South Africa. Electronic address: e.prinsloo@ru.ac.za.
authors.forename: Earl
authors.initials: E
authors.lastname: Prinsloo
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects; D050156:Adipogenesis / Q000187:drug effects; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects; D003199:Computer Systems; D003584:Cytological Techniques / Q000295:instrumentation*; D003907:Dexamethasone / Q000494:pharmacology; D017097:Electric Impedance; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000494:pharmacology
chemical_list: D007328:Insulin; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D003907:Dexamethasone; D015056:1-Methyl-3-isobutylxanthine
keywords: 3T3-L1 cells; Adipogenesis; Impedance; Real-time analysis