title: A STD-NMR study of the interaction of the Anabaena ferredoxin-NADP+ reductase with the coenzyme.
abstract: Ferredoxin-NADP+ reductase (FNR) catalyzes the electron transfer from ferredoxin to NADP+ via its flavin FAD cofactor. To get further insights in the architecture of the transient complexes produced during the hydride transfer event between the enzyme and the NADP+ coenzyme we have applied NMR spectroscopy using Saturation Transfer Difference (STD) techniques to analyze the interaction between FNRox and the oxidized state of its NADP+ coenzyme. We have found that STD NMR, together with the use of selected mutations on FNR and of the non-FNR reacting coenzyme analogue NAD+, are appropriate tools to provide further information about the the interaction epitope.
authors.affiliation: Instituto de Investigaciones Químicas, CSIC, Americo Vespucio, 49, Sevilla 41092, Spain. laravaantonini@yahoo.com.ar.
authors.forename: Lara V
authors.initials: LV
authors.lastname: Antonini
authors.affiliation: Instituto de Investigaciones Químicas, CSIC, Americo Vespucio, 49, Sevilla 41092, Spain. jrp8@st-andrews.ac.uk.
authors.forename: José R
authors.initials: JR
authors.lastname: Peregrina
authors.affiliation: Instituto de Investigaciones Químicas, CSIC, Americo Vespucio, 49, Sevilla 41092, Spain. jesus@iiq.csic.es.
authors.forename: Jesús
authors.initials: J
authors.lastname: Angulo
authors.affiliation: Instituto de Investigaciones Químicas, CSIC, Americo Vespucio, 49, Sevilla 41092, Spain. mmedina@unizar.es.
authors.forename: Milagros
authors.initials: M
authors.lastname: Medina
authors.affiliation: Instituto de Investigaciones Químicas, CSIC, Americo Vespucio, 49, Sevilla 41092, Spain. laravaantonini@yahoo.com.ar.
authors.forename: Pedro M
authors.initials: PM
authors.lastname: Nieto
mesh_terms: D019943:Amino Acid Substitution; D017033:Anabaena / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D020134:Catalytic Domain; D003067:Coenzymes / Q000737:chemistry*; D005287:Ferredoxin-NADP Reductase / Q000737:chemistry* / Q000235:genetics; D007700:Kinetics; D008958:Models, Molecular; D009249:NADP / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding
chemical_list: D001426:Bacterial Proteins; D003067:Coenzymes; D009249:NADP; D005287:Ferredoxin-NADP Reductase