title: The LOSS OF APOMEIOSIS (LOA) locus in Hieracium praealtum can function independently of the associated large-scale repetitive chromosomal structure.
abstract: Apomixis or asexual seed formation in Hieracium praealtum (Asteraceae) is controlled by two independent dominant loci. One of these, the LOSS OF APOMEIOSIS (LOA) locus, controls apomixis initiation, mitotic embryo sac formation (apospory) and suppression of the sexual pathway. The LOA locus is found near the end of a hemizygous chromosome surrounded by extensive repeats extending along the chromosome arm. Similar apomixis-carrying chromosome structures have been found in some apomictic grasses, suggesting that the extensive repetitive sequences may be functionally relevant to apomixis. Fluorescence in situ hybridization (FISH) was used to examine chromosomes of apomeiosis deletion mutants and rare recombinants in the critical LOA region arising from a cross between sexual Hieracium pilosella and apomictic H. praealtum. The combined analyses of aposporous and nonaposporous recombinant progeny and chromosomal karyotypes were used to determine that the functional LOA locus can be genetically separated from the very extensive repeat regions found on the LOA-carrying chromosome. The large-scale repetitive sequences associated with the LOA locus in H. praealtum are not essential for apospory or suppression of sexual megasporogenesis (female meiosis).
authors.affiliation: Laboratory of Plant Molecular Genetics, Division of Natural Science, Osaka Kyoiku University, Kashiwara, Osaka, 582-8582, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Kotani
authors.affiliation: Commonwealth Scientific and Industrial Research Organization (CSIRO), Plant Industry, Waite Campus, Hartley Grove, Urrbrae, Adelaide, SA, 5064, Australia.
authors.forename: Steven T
authors.initials: ST
authors.lastname: Henderson
authors.affiliation: Laboratory of Plant Molecular Genetics, Division of Natural Science, Osaka Kyoiku University, Kashiwara, Osaka, 582-8582, Japan.
authors.forename: Go
authors.initials: G
authors.lastname: Suzuki
authors.affiliation: Commonwealth Scientific and Industrial Research Organization (CSIRO), Plant Industry, Waite Campus, Hartley Grove, Urrbrae, Adelaide, SA, 5064, Australia.
authors.forename: Susan D
authors.initials: SD
authors.lastname: Johnson
authors.affiliation: Commonwealth Scientific and Industrial Research Organization (CSIRO), Plant Industry, Waite Campus, Hartley Grove, Urrbrae, Adelaide, SA, 5064, Australia.
authors.forename: Takashi
authors.initials: T
authors.lastname: Okada
authors.affiliation: Commonwealth Scientific and Industrial Research Organization (CSIRO), Plant Industry, Waite Campus, Hartley Grove, Urrbrae, Adelaide, SA, 5064, Australia.
authors.forename: Hayley
authors.initials: H
authors.lastname: Siddons
authors.affiliation: Laboratory of Plant Molecular Genetics, Division of Natural Science, Osaka Kyoiku University, Kashiwara, Osaka, 582-8582, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Mukai
authors.affiliation: Commonwealth Scientific and Industrial Research Organization (CSIRO), Plant Industry, Waite Campus, Hartley Grove, Urrbrae, Adelaide, SA, 5064, Australia.
authors.forename: Anna M G
authors.initials: AMG
authors.lastname: Koltunow
mesh_terms: D019659:Asteraceae / Q000166:cytology / Q000235:genetics* / Q000502:physiology; D032461:Chromosomes, Plant / Q000235:genetics*; D056426:Genetic Loci / Q000235:genetics*; D018745:Genome, Plant / Q000235:genetics; D008677:Metaphase / Q000235:genetics; D020161:Physical Chromosome Mapping; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012098:Reproduction / Q000235:genetics; D017384:Sequence Deletion
keywords: Hieracium; apomeiosis; apomixis; apospory; chromosome structure; fluorescence in situ hybridization (FISH)