title: [Comparative studies on different methods for laboratory diagnosis of pertussis].
abstract: OBJECTIVE
  To confirm the clinically suspected pertussis cases(<1 years old) through laboratory methods.
  
  
  METHODS
  From December, 2011 to December, 2012, patients with clinically suspected pertussis from Xi'an Children's Hospital were sampled, with their nasopharyngeal swabs collected, blood samples cultured and pertussis toxin IgG detected by PCR.
  
  
  RESULTS
  were analyzed, using SPSS 16.0 software.
  
  
  RESULTS
  100 out of the 148 cases were laboratorial confirmed. 3, 88 and 34 cases were positive, through culture, PCR or pertussis toxin IgG respectively. 22 cases were both PCR and pertussis toxin IgG positive. There were significant differences between the results of IS481 PCR, days from the onset of symptoms (P < 0.01) and results of PT-IgG with the days from onset of symptoms (P < 0.01).
  
  
  CONCLUSIONS
  Since the sensitivity of culture on pertussis was low, diagnosis on the disease should be linked to the results from PCR, PT-IgG and the days from onset of symptoms.
authors.affiliation: Department of Vaccine Preventable Diseases, Xi'an Center for Disease Control and Prevention, Xi'an 710054, China.
authors.forename: Zeng-guo
authors.initials: ZG
authors.lastname: Wang
authors.affiliation: Department of Vaccine Preventable Diseases, Xi'an Center for Disease Control and Prevention, Xi'an 710054, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Vaccine Preventable Diseases, Xi'an Center for Disease Control and Prevention, Xi'an 710054, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Infectious Diseases, Xi'an Children Hospital.
authors.forename: Xiao-guai
authors.initials: XG
authors.lastname: Liu
authors.affiliation: Department of Vaccine Preventable Diseases, Xi'an Center for Disease Control and Prevention, Xi'an 710054, China.
authors.forename: Yan-li
authors.initials: YL
authors.lastname: Xi
authors.affiliation: Department of Infectious Diseases, Xi'an Children Hospital.
authors.forename: Ling-xia
authors.initials: LX
authors.lastname: Lei
authors.affiliation: Department of Infectious Diseases, Xi'an Children Hospital. Email: lyr640101@163.com.
authors.forename: Ya-rong
authors.initials: YR
authors.lastname: Li
mesh_terms: D019411:Clinical Laboratory Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D014917:Whooping Cough / Q000175:diagnosis*