title: Ileal interposition and viability of pancreatic islets transplanted into intramuscular site of diabetic rats.
abstract: BACKGROUND
  Assuming that ileal stimulation by food may increase incretin secretion, we aimed to investigate whether ileal interposition obtains adequate pancreatic islet viability and function after intramuscular islet transplantation in diabetic rats.
  
  
  METHODS
  We investigated four groups of eight Wistar rats: ileal interposition + islet transplantation, islet transplantation, ileal interposition, and diabetic control. All rats were subjected to streptozotocin-induced diabetes. We used the C-peptide/glucose ratio and islet image to investigate beta cell mass, and plasma glucagon like peptide-1 (GLP-1) measure.
  
  
  RESULTS
  Ileal interposition was effective in preserving function and increasing islet mass in animals with islets transplanted into alginate microcapsules. The plasma GLP-1 level in the diabetic control rats was a basal concentration (4.1 ± 1.2 pM). GLP-1 level after ileal interposition + islet transplantation (12.3 ± 3.3 pM) was significantly higher (p < .05) than in the islet transplantation group (8.2 ± 2.4 pM) and ileal interposition group rats (7.6 ± 1.8 pM).
  
  
  CONCLUSIONS
  Ileal interposition positively influenced beta cell viability after intramuscular transplantation of pancreatic islets in diabetic rats.
authors.affiliation: Department of Surgery, Federal University of Rio Grande do Norte, Natal , Brazil.
authors.forename: Irami
authors.initials: I
authors.lastname: Araújo-Filho
authors.forename: Amália Cínthia Meneses
authors.initials: AC
authors.lastname: Rêgo
authors.forename: Ítalo  Medeiros
authors.initials: Í
authors.lastname: Azevedo
authors.forename: Marília Daniela Ferreira
authors.initials: MD
authors.lastname: Carvalho
authors.forename: Aldo Cunha
authors.initials: AC
authors.lastname: Medeiros
mesh_terms: D000009:Abdominal Muscles / Q000601:surgery; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000601:surgery*; D052216:Glucagon-Like Peptide 1 / Q000097:blood* / Q000378:metabolism; D006085:Graft Survival; D007082:Ileum / Q000601:surgery*; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D011897:Random Allocation; D017208:Rats, Wistar
chemical_list: D052216:Glucagon-Like Peptide 1
keywords: C-peptide; beta cell mass; diabetes; ileal interposition; islet transplantation; rat