title: Microfluidic immunomagnetic cell separation using integrated permanent micromagnets.
abstract: In this paper, we demonstrate the possibility to trap and sort labeled cells under flow conditions using a microfluidic device with an integrated flat micro-patterned hard magnetic film. The proposed technique is illustrated using a cell suspension containing a mixture of Jurkat cells and HEK (Human Embryonic Kidney) 293 cells. Prior to sorting experiments, the Jurkat cells were specifically labeled with immunomagnetic nanoparticles, while the HEK 293 cells were unlabeled. Droplet-based experiments demonstrated that the Jurkat cells were attracted to regions of maximum stray field flux density while the HEK 293 cells settled in random positions. When the mixture was passed through a polydimethylsiloxane (PDMS) microfluidic channel containing integrated micromagnets, the labeled Jurkat cells were selectively trapped under fluid flow, while the HEK cells were eluted towards the device outlet. Increasing the flow rate produced a second eluate much enriched in Jurkat cells, as revealed by flow cytometry. The separation efficiency of this biocompatible, compact micro-fluidic separation chamber was compared with that obtained using two commercial magnetic cell separation kits.
authors.affiliation: Ampère Laboratory, Ecole Centrale Lyon, CNRS, UMR 5005, 69134 Ecully, France.
authors.forename: O
authors.initials: O
authors.lastname: Osman
authors.affiliation: Ampère Laboratory, Ecole Centrale Lyon, CNRS, UMR 5005, 69134 Ecully, France.
authors.forename: S
authors.initials: S
authors.lastname: Toru
authors.affiliation: Univ. Grenoble Alpes, Inst NEEL, F-38042 Grenoble, France and CNRS, Inst NEEL, F-38042 Grenoble, France ; SPCTS, CNRS - University of Limoges, 12 Rue Atlantis, 87068 Limoges, France.
authors.forename: F
authors.initials: F
authors.lastname: Dumas-Bouchiat
authors.affiliation: Univ. Grenoble Alpes, Inst NEEL, F-38042 Grenoble, France and CNRS, Inst NEEL, F-38042 Grenoble, France.
authors.forename: N M
authors.initials: NM
authors.lastname: Dempsey
authors.affiliation: Ampère Laboratory, Ecole Centrale Lyon, CNRS, UMR 5005, 69134 Ecully, France.
authors.forename: N
authors.initials: N
authors.lastname: Haddour
authors.affiliation: Univ. Grenoble Alpes, Inst NEEL, F-38042 Grenoble, France and CNRS, Inst NEEL, F-38042 Grenoble, France ; G2Elab, Grenoble Université, BP 46, 38402 St. Martin d'Hères, France.
authors.forename: L-F
authors.initials: LF
authors.lastname: Zanini
authors.affiliation: Ampère Laboratory, Ecole Centrale Lyon, CNRS, UMR 5005, 69134 Ecully, France.
authors.forename: F
authors.initials: F
authors.lastname: Buret
authors.affiliation: G2Elab, Grenoble Université, BP 46, 38402 St. Martin d'Hères, France.
authors.forename: G
authors.initials: G
authors.lastname: Reyne
authors.affiliation: Ampère Laboratory, Université Lyon 1, CNRS, UMR 5005, 69622 Villeurbanne, France.
authors.forename: M
authors.initials: M
authors.lastname: Frénéa-Robin