title: Novel real-time PCR assays using TaqMan minor groove binder probes for identification of fecal carriage of Streptococcus bovis/Streptococcus equinus complex from rectal swab specimens.
abstract: Real-time PCR based on the recN and gyrB genes was developed to detect four Streptococcus bovis/Streptococcus equinus complex (SBEC) subspecies from rectal swab specimens. The overall prevalence was 35.2%: Streptococcus gallolyticus subsp. gallolyticus (11.1%), S. gallolyticus subsp. pasteurianus (13%), Streptococcus infantarius subsp. coli (20.4%), and S. infantarius subsp. infantarius (11.1%). To conclude, these real-time PCR assays provide a reliable molecular method to detect SBEC pathogenic subspecies from rectal swab specimens.
authors.affiliation: Health Sciences Federal University of Porto Alegre (UFCSPA), Porto Alegre, RS, Brazil.
authors.forename: Paulo Guilherme Markus
authors.initials: PG
authors.lastname: Lopes
authors.forename: Vlademir Vicente
authors.initials: VV
authors.lastname: Cantarelli
authors.forename: Grasiela
authors.initials: G
authors.lastname: Agnes
authors.forename: Ane Micheli
authors.initials: AM
authors.lastname: Costabeber
authors.forename: Pedro Alves
authors.initials: PA
authors.lastname: d'Azevedo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001426:Bacterial Proteins / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods*; D002353:Carrier State / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology; D027081:DNA Gyrase / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013290:Streptococcal Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology; D013291:Streptococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D015345:Oligonucleotide Probes; D004262:DNA Restriction Enzymes; C047879:RecN protein, Bacteria; D027081:DNA Gyrase