title: Performance of enzymatic fuel cell in cell culture.
abstract: Here we present the very first study of an enzymatic fuel cell (EFC) in a cell culture. An EFC with Corynascus thermophilus cellobiose dehydrogenase (CDH) based bioanode and Myrothecium verrucaria bilirubin oxidase (BOx) based biocathode was constructed at the bottom of a medusa cell culture plate. The constructed EFC had a power density of up to 25 μW cm(-2) at 0.5 V potential in simple buffer solution and in cell culturing medium. L929 murine fibroblast cells were seeded on top of the EFC and possible effects of the EFC on the cells and vice versa were studied. It was shown that on average the power of the EFC drops by about 70% under a nearly confluent layer of cells. The EFC appeared to have a toxic effect on the L929 cell line. It was concluded that the bioanode, consisting of CDH, produced hydrogen peroxide at toxic concentrations. However, the toxic effect was circumvented by co-immobilizing catalase on the bioanode.
authors.affiliation: Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, 20506 Malmö, Sweden. Electronic address: peter.lamberg@mah.se.
authors.forename: P
authors.initials: P
authors.lastname: Lamberg
authors.affiliation: Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, 20506 Malmö, Sweden; Laboratory of Chemical Enzymology, A.N. Bach Institute of Biochemistry, RAS, 119071 Moscow, Russia.
authors.forename: S
authors.initials: S
authors.lastname: Shleev
authors.affiliation: Department of Food Sciences and Technology, Food Biotechnology Laboratory, BOKU-University of Natural Resources and Life Sciences, A-1190 Vienna, Austria.
authors.forename: R
authors.initials: R
authors.lastname: Ludwig
authors.affiliation: Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, 20506 Malmö, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Arnebrant
authors.affiliation: Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, 20506 Malmö, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Ruzgas
mesh_terms: D000818:Animals; D061249:Batch Cell Culture Techniques / Q000295:instrumentation*; D001674:Bioelectric Energy Sources; D015374:Biosensing Techniques / Q000295:instrumentation*; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry / Q000493:pharmacokinetics*; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D004566:Electrodes; D004735:Energy Transfer; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005347:Fibroblasts / Q000502:physiology*; D051379:Mice; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000737:chemistry / Q000493:pharmacokinetics*
chemical_list: D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase
keywords: Bilirubin oxidase; Biofuel cell; Cellobiose dehydrogenase; Direct electron transfer; L929 cell line; Toxicity