title: Rapid and serum-insensitive endocytotic delivery of proteins using biotinylated polymers attached via multivalent hydrophobic anchors.
abstract: We have designed biotinylated polymers as synthetic receptors that have multiple alkyl groups for endocytotic delivery of target proteins. The polymers were stably attached to a cell surface via multivalent anchoring. The presented biotin was bound to streptavidin (SA) on the cell surface, and, via an endocytotic pathway, the cell rapidly internalized the biotinylated polymer/SA complex. The cell's uptake of the complex was not inhibited by the presence of 10% fetal bovine serum, and its efficacy for the uptake of SA was the highest when compared with commercial reagents and single-anchored-type synthetic receptors. The synthetic receptor-mediated endocytosis can be used generally for other kind of protein by using SA as an adaptor molecule between a target protein and the cell-surface presented biotin.
authors.affiliation: Department of Applied Chemistry, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Kyohei
authors.initials: K
authors.lastname: Tobinaga
authors.affiliation: Department of Applied Chemistry, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Cuicui
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Applied Chemistry, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Takeo
authors.affiliation: Department of Applied Chemistry, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Matsuda
authors.affiliation: Department of Applied Chemistry, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Nagai
authors.affiliation: Department of Applied Chemistry, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan; Center for Future Chemistry, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Takuro
authors.initials: T
authors.lastname: Niidome
authors.affiliation: Department of Applied Chemistry, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Tatsuhiro
authors.initials: T
authors.lastname: Yamamoto
authors.affiliation: Department of Applied Chemistry, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan; Center for Future Chemistry, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kishimura
authors.affiliation: Department of Applied Chemistry, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan; Center for Future Chemistry, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address: mori.takeshi.880@m.kyushu-u.ac.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Mori
authors.affiliation: Department of Applied Chemistry, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan; Center for Future Chemistry, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address: ykatatcm@mail.cstm.kyushu-u.ac.jp.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Katayama
mesh_terms: D001710:Biotin / Q000737:chemistry*; D019921:Biotinylation; D003911:Dextrans / Q000737:chemistry*; D004705:Endocytosis; D005029:Ethylenediamines / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D020014:K562 Cells; D010047:Ovalbumin / Q000008:administration & dosage* / Q000737:chemistry; D010168:Palmitates / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry; D044967:Serum; D019809:Streptavidin / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D003911:Dextrans; D005029:Ethylenediamines; D010168:Palmitates; D012235:Rhodamines; C031234:ethylenediamine; D001710:Biotin; C032023:palmitoyl chloride; D010047:Ovalbumin; D019809:Streptavidin; D016650:Fluorescein-5-isothiocyanate
keywords: Dextran; Drug delivery; Membrane; Protein