title: Determination of losartan potassium, quinapril hydrochloride and hydrochlorothiazide in pharmaceutical preparations using derivative spectrophotometry and chromatographic-densitometric method.
abstract: Two methods, spectrophotometric and chromatographic-densitometric ones, were developed for determination of losartan potassium, quinapril hydrochloride and hydrochlorothiazide in pharmaceutical preparations. Spectrophotometric method involved derivative spectrophotometry and zero order spectrophotometry. The measurements were carried out at lambda = 224.0 nm for quinapril, lambda = 261.0 nm for hydrochlorothiazide and lambda = 270.0 nm for losartan when the derivative spectrophotometry was applied and lambda = 317.0 nm when zero order spectrophotometry was applied for the determination of hydrochlorothiazide. In chromatographic-densitometric studies high performance thin layer chromatography (HPTLC) plates were used as stationary phase and a mixture of solvents n-butanol : acetic acid : water (15 : 5 : 1, v/v/v) as mobile phase. Under the established conditions good resolution of examined constituents was obtained. Retardation factor for quinapril hydrochloride was R(f) - 0.70, for losartan potassium R(f) - 0.85 and for hydrochlorothiazide R(f) - 0.78. The developed methods are characterized by high sensitivity and accuracy. For quantitative analysis, densitometric measurements were carried out at lambda = 218.0 nm for quinapril, lambda = 275.0 nm for hydrochlorothiazide and = 232.0 nm for losartan.
authors.affiliation: Department of Inorganic and Analytical Chemistry, Collegium Medium, Jagiellonian University, 9 Medyczna St., 30-688 Kraków, Poland. mariusz.stolarczyk@uj.edu.pl
authors.forename: Mariusz
authors.initials: M
authors.lastname: Stolarczyk
authors.affiliation: Department of Inorganic and Analytical Chemistry, Collegium Medium, Jagiellonian University, 9 Medyczna St., 30-688 Kraków, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Maślanka
authors.affiliation: Department of Inorganic and Analytical Chemistry, Collegium Medium, Jagiellonian University, 9 Medyczna St., 30-688 Kraków, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Apola
authors.affiliation: Department of Inorganic and Analytical Chemistry, Collegium Medium, Jagiellonian University, 9 Medyczna St., 30-688 Kraków, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Krzek
mesh_terms: D000959:Antihypertensive Agents / Q000032:analysis*; D002138:Calibration; D002855:Chromatography, Thin Layer / Q000592:standards; D003720:Densitometry / Q000592:standards; D004338:Drug Combinations; D006852:Hydrochlorothiazide / Q000032:analysis*; D057230:Limit of Detection; D016014:Linear Models; D019808:Losartan / Q000032:analysis*; D000077583:Quinapril; D012015:Reference Standards; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000592:standards; D044005:Tetrahydroisoquinolines / Q000032:analysis*
chemical_list: D000959:Antihypertensive Agents; D004338:Drug Combinations; D012997:Solvents; D044005:Tetrahydroisoquinolines; D006852:Hydrochlorothiazide; D019808:Losartan; D000077583:Quinapril