title: [The effect of PPARalpha signal channel on atorvastatin inhibiting MMP-9 expression in aging myocytes].
abstract: OBJECTIVE
  To investigate the correlation between atorvastatin inhibiting the expression level of matrix metalloproteinase 9 (MMP-9) and peroxisome proliferator activated receptor alpha (PPARalpha) signal channel in myocyte of aging rat.
  
  
  METHODS
  Primary cultures of myocyte were got ten from aging rats. Myocyte were divided into control group, DMSO group, atorvastatin group, atorvastatin plus GW6471 group, which treated respectively by cell culture medium, DMSO, atorvastatin, atorvastatin plus GW6471. The expression of MMP-9 mRNA was evaluated by RT-PCR, and content of MMP-9 protein was detected by Western blot.
  
  
  RESULTS
  (1) There was no difference between control group and DMSO group in level of MMP-9 mRNA and protein expression (P > 0.05); (2) The level of MMP-9 mRNA and MMP-9 protein expression in atorvastatin group were significantly lower than those in control group (P < 0.01); (3) Both level of MMP-9 mRNA and protein expression in atorvastatin plus GW6471 group were significantly higher than those in atorvastatin group (P < 0.05), but were still lower than those in control group (P < 0.05).
  
  
  CONCLUSIONS
  Atorvastatin inhibit MMP-9 expression of aging myocytes by PPARalpha signal channel.
authors.affiliation: Aviation and Nautical Medical Center, Navy General Hospital, Beijing 100048, China. leihan999@sina.com
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.affiliation: The Second Department of Geriatric Cardiology, PLA General Hospital, Beijing 100048, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Ye
authors.affiliation: Aviation and Nautical Medical Center, Navy General Hospital, Beijing 100048, China.
authors.forename: Ming-Gao
authors.initials: MG
authors.lastname: Li
mesh_terms: D000375:Aging; D000818:Animals; D000069059:Atorvastatin; D002478:Cells, Cultured; D006538:Heptanoic Acids / Q000494:pharmacology*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D032342:Muscle Cells / Q000187:drug effects* / Q000378:metabolism*; D010080:Oxazoles / Q000494:pharmacology; D047493:PPAR alpha / Q000378:metabolism*; D011758:Pyrroles / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D014443:Tyrosine / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: C449302:GW 6471; D006538:Heptanoic Acids; D010080:Oxazoles; D047493:PPAR alpha; D011758:Pyrroles; D014443:Tyrosine; D000069059:Atorvastatin; D020780:Matrix Metalloproteinase 9; C572337:Mmp9 protein, rat