title: Synthesis, characterization, and DNA binding studies of a new Pt(II) complex containing the drug Levetiracetam: combining experimental and computational methods.
abstract: The water-soluble platinum(II) complex, [Pt(LV)(dmso)Cl]Cl (LV is Levetiracetam an anti-epileptic drug belonging to the pyrrolidine family) was synthesized and characterized using physico-chemical, spectroscopic and computational methods. Multi-spectroscopic methods used to study the interaction of Pt(II) complex with calf thymus (ct-DNA) in Tris-HCl buffer solution (pH 7.4). The Pt(II) complex was found to be a minor groove binder with a preference for the A-T region compared to the G-C region. This was substantiated by displacement studies with Hoechst 33258, a known minor groove binder. The mean binding constant obtained from the spectroscopic techniques was 7 × 10(4) M(-1). In comparison with the previous study, the binding constant of Pt(II) complex is 15 times greater than the corresponding value of levetiracetam. These results indicate that the binding affinity of Pt(II) complex is stronger than levetiracetam.
authors.affiliation: Department of Inorganic Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran, NahidShahabadi@yahoo.com.
authors.forename: Nahid
authors.initials: N
authors.lastname: Shahabadi
authors.forename: Saba
authors.initials: S
authors.lastname: Hadidi
authors.forename: Avat Arman
authors.initials: AA
authors.lastname: Taherpour
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry*; D001665:Binding Sites; D001667:Binding, Competitive; D006690:Bisbenzimidazole / Q000737:chemistry; D002021:Buffers; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry*; D007700:Kinetics; D000077287:Levetiracetam; D009944:Organoplatinum Compounds / Q000737:chemistry*; D010889:Piracetam / Q000031:analogs & derivatives* / Q000737:chemistry; D013816:Thermodynamics
chemical_list: D000927:Anticonvulsants; D000970:Antineoplastic Agents; D002021:Buffers; D007364:Intercalating Agents; D009944:Organoplatinum Compounds; D000077287:Levetiracetam; D004247:DNA; C427203:calf thymus DNA; D006690:Bisbenzimidazole; D010889:Piracetam