title: Impact of static cold storage VS hypothermic machine preservation on ischemic kidney graft: inflammatory cytokines and adhesion molecules as markers of ischemia/reperfusion tissue damage. Our preliminary results.
abstract: At the present time, deceased heart-beating donor kidney allografts are usually stored cold. Extended-criteria donor (ECD) grafts show higher sensitivity to ischemia-reperfusion damage than standard kidneys. The increasing use of marginal organs in clinical transplantation urgently requires a more effective preservation system. Pulsatile hypothermic machine perfusion has shown major advantages over static cold storage in terms of reduced organ injury during preservation and improved early graft function. This preliminary study aims to compare pulsatile hypothermic machine perfusion and static cold storage of kidney allografts, outlining differences in the levels of early inflammatory cytokines (TNF-α, IL-2 and IL-1β) and soluble intracellular adhesion molecule (sICAM-1) in perfusion and preservation liquid.
authors.affiliation: Unit of General Surgery and Organ Transplantation, Insubria University, Varese, Italy; Unit of Vascular Surgery, Circolo teaching Hospital, Insubria University, Varese, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Tozzi
authors.affiliation: Unit of General Surgery and Organ Transplantation, Insubria University, Varese, Italy; Unit of General Surgery, Circolo teaching Hospital, Insubria University, Varese, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Franchin
authors.affiliation: Unit of General Surgery and Organ Transplantation, Insubria University, Varese, Italy; Unit of General Surgery, Circolo teaching Hospital, Insubria University, Varese, Italy.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Soldini
authors.affiliation: Unit of General Surgery and Organ Transplantation, Insubria University, Varese, Italy; Unit of General Surgery, Circolo teaching Hospital, Insubria University, Varese, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Ietto
authors.affiliation: Unit of General Surgery and Organ Transplantation, Insubria University, Varese, Italy; Unit of General Surgery, Circolo teaching Hospital, Insubria University, Varese, Italy.
authors.forename: Corrado
authors.initials: C
authors.lastname: Chiappa
authors.affiliation: Unit of General Surgery and Organ Transplantation, Insubria University, Varese, Italy; Unit of Vascular Surgery, Circolo teaching Hospital, Insubria University, Varese, Italy.
authors.forename: Emanuele
authors.initials: E
authors.lastname: Maritan
authors.affiliation: Unit of General Surgery and Organ Transplantation, Insubria University, Varese, Italy; Unit of Vascular Surgery, Circolo teaching Hospital, Insubria University, Varese, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Villa
authors.affiliation: Unit of General Surgery and Organ Transplantation, Insubria University, Varese, Italy; Unit of General Surgery, Circolo teaching Hospital, Insubria University, Varese, Italy.
authors.forename: Giulio
authors.initials: G
authors.lastname: Carcano
authors.affiliation: Unit of General Surgery, Circolo teaching Hospital, Insubria University, Varese, Italy.
authors.forename: Renzo
authors.initials: R
authors.lastname: Dionigi
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000032:analysis*; D003080:Cold Temperature; D016207:Cytokines / Q000032:analysis*; D005260:Female; D006801:Humans; D007511:Ischemia / Q000503:physiopathology; D007668:Kidney / Q000098:blood supply*; D016030:Kidney Transplantation / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009926:Organ Preservation / Q000379:methods*; D011446:Prospective Studies; D015427:Reperfusion Injury / Q000378:metabolism*; D014022:Tissue Survival
chemical_list: D015415:Biomarkers; D016207:Cytokines
keywords: Hypothermic perfusion machine; Ischemia/reperfusion injury; Kidney transplant; Static cold storage