title: Development of an immunochromatographic lateral flow device for rapid diagnosis of Vibrio cholerae O1 serotype Ogawa.
abstract: OBJECTIVE
  Cholera is an acute malignant infectious disease caused by the bacteria Vibrio cholerae leading to severe dehydrating diarrhea and vomiting, even high rates of mortality in some cases. However, the prevention of the epidemic disease is achievable if proper sanitation practices are followed, provided the accurate and prompt diagnosis of each prevalent serotype in cholera epidemic. The current gold standard of bacterial culture is inadequate for rapid diagnosis. Our aim is to develop an immunochromatographic test format for O1 serotype Ogawa diagnosis and provide the need for better epidemic prevention and early response.
  
  
  METHODS
  The monoclonal antibodies were raised in conventional method and subsequently screened for a match pair. A variety of related and unrelated bacteria strains recruited were employed to test their sensitivity, specificity etc. by indirect ELISA. The human fecal samples were used to test the final lateral-flow device product to satisfy the measurement requirement.
  
  
  RESULTS
  A new monoclonal antibody (McAb) pair, named IXiao₃G₆ and IXiao₁D₉, was generated, which is specifically against V. cholerae O1 serotype Ogawa. Additionally, we developed an immunochromatographic lateral flow device (LFD) using this McAb pair for the highly specific and rapid (5 min) detection of Ogawa.
  
  
  CONCLUSIONS
  Our product has advantages of simplicity and precision, and can benefit the scene and elementary medical institutions.
authors.affiliation: The Clinical Laboratory Center, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Weixian
authors.initials: W
authors.lastname: Chen
authors.affiliation: Artron BioResearch Inc., Burnaby, British Columbia, Canada.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Artron BioResearch Inc., Burnaby, British Columbia, Canada.
authors.forename: Gang
authors.initials: G
authors.lastname: Lu
authors.affiliation: The Institute for Viral Hepatitis, Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
authors.forename: Zuowei
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: The Clinical Laboratory Center, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wu
authors.affiliation: The Blood Transfusion Department, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.affiliation: The Blood Transfusion Department, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Guiping
authors.initials: G
authors.lastname: Xu
authors.affiliation: The Institute for Viral Hepatitis, Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
authors.forename: An
authors.initials: A
authors.lastname: He
authors.affiliation: The Institute for Viral Hepatitis, Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zheng
authors.affiliation: The Blood Transfusion Department, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China. Electronic address: jian0102@hotmail.com.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification; D000918:Antibody Specificity / Q000276:immunology; D002771:Cholera / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods*; D005260:Female; D007114:Immunization; D008807:Mice, Inbred BALB C; D011933:Reagent Kits, Diagnostic; D012212:Rheology / Q000295:instrumentation*; D012703:Serotyping; D041241:Vibrio cholerae O1 / Q000145:classification* / Q000302:isolation & purification*
chemical_list: D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic
keywords: Cholera; Lateral flow device; Monoclonal antibody; Ogawa; Rapid flow test