title: Secondary structure of the Irf7 5'-UTR, analyzed using SHAPE (selective 2'-hydroxyl acylation analyzed by primer extension).
abstract: OASL1 is a member of the 2'-5'-oligoadenylate synthetase (OAS) family and promotes viral clearance by activating RNase L. OASL1 interacts with the 5'-untranslated region (UTR) of interferon regulatory factor 7 (Irf7) and inhibits its translation. To identify the secondary structure required for OASL1 binding, we examined the 5'-UTR of the Irf7 transcript using "selective 2'-hydroxyl acylation analyzed by primer extension" (SHAPE). SHAPE takes advantage of the selective acylation of residues in single-stranded regions by 1-methyl-7-nitroisatoic anhydride (1M7). We found five major acylation sites located in, or next to, predicted single-stranded regions of the Irf7 5'-UTR. These results demonstrate the involvement of the stem structure of the Irf7 5'-UTR in the regulation of Irf7 translation, mediated by OASL1.
authors.affiliation: Departments of Integrated OMICs for Biomedical Science, Yonsei University, Seoul 120-749, Korea.
authors.forename: Yun-Mi
authors.initials: YM
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, Yonsei University, Seoul 120-749, Korea.
authors.forename: Won-Young
authors.initials: WY
authors.lastname: Choi
authors.affiliation: Departments of Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors.forename: Chang-Mok
authors.initials: CM
authors.lastname: Oh
authors.affiliation: Departments of Integrated OMICs for Biomedical Science, Yonsei University, Seoul 120-749, Korea.
authors.forename: Gyoon-Hee
authors.initials: GH
authors.lastname: Han
authors.affiliation: Departments of Integrated OMICs for Biomedical Science, Yonsei University, Seoul 120-749, Korea.
authors.forename: Young-Joon
authors.initials: YJ
authors.lastname: Kim
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D000215:Acylation; D000241:Adenosine / Q000378:metabolism; D001483:Base Sequence; D017931:DNA Primers / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006900:Hydroxylation; D050839:Interferon Regulatory Factor-7 / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010078:Oxazines / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: C585663:1-methyl-7-nitroisatoic anhydride; D020121:5' Untranslated Regions; D017931:DNA Primers; D050839:Interferon Regulatory Factor-7; D010078:Oxazines; D000241:Adenosine