title: DNAzyme self-assembled gold nanorods-based FRET or polarization assay for ultrasensitive and selective detection of copper(II) ion.
abstract: In the paper, we have constructed a very simple, sensitive and promising assay for fluorescence biosensor detection of Cu(2+) in aqueous solutions based on FRET between gold nanorods (AuNRs) and the 3'-TAMRA-labeled substrate strand (Sub) of DNAzyme. The fluorescence of the Sub is quenched when the substrate strand-DNAzyme strand (Sub-Enz) duplex is adsorbed on AuNRs surface through electrostatic interaction. In the presence of Cu(2+), the fluorescence is restored due to the decrease of FRET efficiency caused by the specific cleavage of the Sub by the DNAzyme (Enz), which weakens the electrostatic interaction between the AuNRs and short 3'-TAMRA-labeled DNA fragment. This method shows a high sensitivity for Cu(2+) with a detection limit of 9.83 pM (S/N=3) and a linear range from 0.016 nM to 40 nM. At the same time, Cu(2+) can be detected sensitively based on the significantly decreased Fluorescent polarization (FP). The detection limit of 8.40 pM is experimentally achieved for Cu(2+).
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China.
authors.forename: Yanlong
authors.initials: Y
authors.lastname: He
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China. Electronic address: birdtjn@sina.com.
authors.forename: Jianniao
authors.initials: J
authors.lastname: Tian
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China.
authors.forename: Juanni
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Chen
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China.
authors.forename: Yixuan
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Hu
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China. Electronic address: yanchunzh@sina.com.
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China.
authors.forename: Shulin
authors.initials: S
authors.lastname: Zhao
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003300:Copper / Q000032:analysis* / Q000737:chemistry; D021881:DNA, Catalytic / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation*; D006046:Gold / Q000737:chemistry*; D007477:Ions; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D012031:Refractometry / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D021881:DNA, Catalytic; D004800:Enzymes, Immobilized; D007477:Ions; D006046:Gold; D003300:Copper
keywords: AuNRs; Copper(II) ion; DNAzyme; Fluorescence resonance energy transfer technique; Fluorescent polarization