title: Penicillin G as a novel chiral selector in capillary electrophoresis.
abstract: The penicillin sub-class of β-lactam antibiotics has not been examined for its enantiodiscriminating abilities in capillary electrophoresis (CE) until date. The present work was therefore designed to evaluate penicillin G potassium salt (PenG) as an ion-pair chiral selector (CS) using CE for its several attributes, namely, high solubility in water and lower alcohols, structure allowing multiple interactions with analytes and cost-effectiveness. Systematic experiments were performed to investigate the effect of composition of background electrolyte, applied voltage and capillary temperature on chiral separation. Baseline resolutions of enantiomers of five basic chiral drugs (namely, darifenacin, citalopram, sertraline, propranolol and metoprolol) were attained using a background electrolyte composed of water:methanol (90:10, v/v) and consisting of 10.7 or 16.1mM CS at 20°C using an applied voltage of 5kV.
authors.affiliation: Department of Chemistry, Yeungnam University, Gyeongsan 712-749, South Korea. Electronic address: drdixit.shuchi@gmail.com.
authors.forename: Shuchi
authors.initials: S
authors.lastname: Dixit
authors.affiliation: Department of Chemistry, Yeungnam University, Gyeongsan 712-749, South Korea. Electronic address: jhpark@ynu.ac.kr.
authors.forename: Jung Hag
authors.initials: JH
authors.lastname: Park
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001572:Benzofurans / Q000032:analysis; D015283:Citalopram / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods; D006863:Hydrogen-Ion Concentration; D000432:Methanol / Q000737:chemistry; D008790:Metoprolol / Q000032:analysis; D010400:Penicillin G / Q000737:chemistry*; D011433:Propranolol / Q000032:analysis; D011759:Pyrrolidines / Q000032:analysis; D020280:Sertraline / Q000032:analysis; D012995:Solubility; D013237:Stereoisomerism; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D001572:Benzofurans; D011759:Pyrrolidines; D015283:Citalopram; D011433:Propranolol; C101207:darifenacin; D008790:Metoprolol; D010400:Penicillin G; D020280:Sertraline; D000432:Methanol
keywords: CIT; CS; Capillary electrophoresis; Chiral selector; DAR; Enantioseparation; METO; MeCN; MeOH; PRO; PenG; Penicillin G; SERT; acetonitrile; chiral selector; citalopram hydrobromide; darifenacin; methanol; metoprolol; penicillin G; propranolol; sertraline hydrochloride