title: A MADS-box gene is specifically expressed in fibers of cotton (Gossypium hirsutum) and influences plant growth of transgenic Arabidopsis in a GA-dependent manner.
abstract: In this study, a cDNA, GhMADS14, encoding a typical MADS-box protein with 223 amino acids was isolated from a cotton cDNA library. Fluorescent microscopy indicated that the GhMADS14 protein was localized in the cell nucleus. GhMADS14 was specifically expressed in the elongating fibers, and its expression was gradually enhanced at early stages of fiber elongation and reached its peak in 9-10 DPA fibers. Overexpression of GhMADS14 in Arabidopsis hindered plant growth. Measurement and statistical analysis revealed that hypocotyl length of GhMADS14 transgenic seedlings was significantly reduced, and the height of the mature transgenic plants was remarkably less than that of the wild type. Furthermore, expression of GA 20-oxidase (AtGA20ox1 and AtGA20ox2) and GA 3-oxidase (AtGA3ox1 and AtGA3ox2) genes was remarkably reduced, whereas AtGA2ox1 and AtGA2ox8 were dramatically up-regulated in the transgenic plants, compared with the wild type. These results suggested that overexpression of GhMADS14 in Arabidopsis may alter expression levels of the genes related to GA biosynthetic and metabolic pathways, resulting in the reduction of endogenous GA amounts in cells. As a result, the transgenic plants grew slowly and display a GA-deficient phenotype.
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China.
authors.forename: Bing-Ying
authors.initials: BY
authors.lastname: Li
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China.
authors.forename: Mo
authors.initials: M
authors.lastname: Li
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China.
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Li
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China.
authors.forename: Ze-Ting
authors.initials: ZT
authors.lastname: Zhang
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China. Electronic address: xbli@mail.ccnu.edu.cn.
authors.forename: Xue-Bao
authors.initials: XB
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D002467:Cell Nucleus; D032561:Cotton Fiber; D018076:DNA, Complementary; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant; D005875:Gibberellins / Q000235:genetics* / Q000378:metabolism; D003368:Gossypium / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D018546:Hypocotyl / Q000254:growth & development; D026161:MADS Domain Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D012639:Seeds; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D005875:Gibberellins; D026161:MADS Domain Proteins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D006899:Mixed Function Oxygenases; C089210:gibberellin, 2-oxoglutarate-oxygen oxidoreductase (20-hydroxylating, oxidizing); C108186:gibberellin 3beta-hydroxylase
keywords: Cotton (Gossypium hirsutum); Fiber development; Gibberellin (GA); MADS-box protein; Plant growth