title: Rare cell isolation and profiling on a hybrid magnetic/size-sorting chip.
abstract: We present a hybrid magnetic/size-sorting (HMSS) chip for isolation and molecular analyses of circulating tumor cells (CTCs). The chip employs both negative and positive cell selection in order to provide high throughput, unbiased CTC enrichment. Specifically, the system utilizes a self-assembled magnet to generate high magnetic forces and a weir-style structure for cell sorting. The resulting device thus can perform multiple functions, including magnetic depletion, size-selective cell capture, and on-chip molecular staining. With such capacities, the HMSS device allowed one-step CTC isolation and single cell detection from whole blood, tested with spiked cancer cells. The system further facilitated the study of individual CTCs for heterogeneity in molecular marker expression.
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St., CPZN 5206, Boston, Massachusetts 02114, USA.
authors.forename: Jaehoon
authors.initials: J
authors.lastname: Chung
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St., CPZN 5206, Boston, Massachusetts 02114, USA.
authors.forename: David
authors.initials: D
authors.lastname: Issadore
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St., CPZN 5206, Boston, Massachusetts 02114, USA.
authors.forename: Adeeti
authors.initials: A
authors.lastname: Ullal
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St., CPZN 5206, Boston, Massachusetts 02114, USA.
authors.forename: Kyungheon
authors.initials: K
authors.lastname: Lee
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St., CPZN 5206, Boston, Massachusetts 02114, USA ; Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Ralph
authors.initials: R
authors.lastname: Weissleder
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St., CPZN 5206, Boston, Massachusetts 02114, USA.
authors.forename: Hakho
authors.initials: H
authors.lastname: Lee