title: In vitro screening test using Leishmania promastigotes stably expressing mCherry protein.
abstract: Transgenic Leishmania major and Leishmania donovani axenic promastigotes constitutively expressing mCherry were used for in vitro antileishmanial drug screening. This method requires minimal sample manipulation and can be easily adapted to automatic drug tests, allowing primary high-throughput screenings without the need for expensive and sophisticated instruments.
authors.affiliation: Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana, USA.
authors.forename: Paola
authors.initials: P
authors.lastname: Vacchina
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Morales
mesh_terms: D004353:Drug Evaluation, Preclinical / Q000379:methods; D066298:In Vitro Techniques; D007893:Leishmania donovani / Q000378:metabolism*; D018320:Leishmania major / Q000378:metabolism*; D008164:Luminescent Proteins / Q000096:biosynthesis*; D014344:Trypanocidal Agents / Q000494:pharmacology; D000097573:Red Fluorescent Protein
chemical_list: D008164:Luminescent Proteins; D014344:Trypanocidal Agents; D000097573:Red Fluorescent Protein