title: Molecular cloning, bioinformatics analysis and expression of insulin-like growth factor 2 from Tianzhu white yak, Bos grunniens.
abstract: The IGF family is essential for normal embryonic and postnatal development and plays important roles in the immune system, myogenesis, bone metabolism and other physiological functions, which makes the study of its structure and biological characteristics important. Tianzhu white yak (Bos grunniens) domesticated under alpine hypoxia environments, is well adapted to survive and grow against severe hypoxia and cold temperatures for extended periods. In this study, a full coding sequence of the IGF2 gene of Tianzhu white yak was amplified by reverse transcription PCR and rapid-amplification of cDNA ends (RACE) for the first time. The cDNA sequence revealed an open reading frame of 450 nucleotides, encoding a protein with 179 amino acids. Its expression in different tissues was also studied by Real time PCR. Phylogenetic tree analysis indicated that yak IGF2 was similar to Bos taurus, and 3D structure showed high similarity with the human IGF2. The putative full CDS of yak IGF2 was amplified by PCR in five tissues, and cDNA sequence analysis showed high homology to bovine IGF2. Moreover the super secondary structure prediction showed a similar 3D structure with human IGF2. Its conservation in sequence and structure has facilitated research on IGF2 and its physiological function in yak.
authors.affiliation: College of Veterinary Medicine, Gansu Agriculture University, Lanzhou 730070, China. quanweizhang@st.gsau.edu.cn.
authors.forename: Quanwei
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Gansu Agriculture University, Lanzhou 730070, China. gongjishang@st.gsau.edu.cn.
authors.forename: Jishang
authors.initials: J
authors.lastname: Gong
authors.affiliation: College of Veterinary Medicine, Gansu Agriculture University, Lanzhou 730070, China. wx.258.h@st.gsau.edu.cn.
authors.forename: Xueying
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Gansu Agriculture University, Lanzhou 730070, China. wx.258.h@st.gsau.edu.cn.
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Wu
authors.affiliation: College of Veterinary Medicine, Gansu Agriculture University, Lanzhou 730070, China. liyalan0819@gmail.com.
authors.forename: Yalan
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Gansu Agriculture University, Lanzhou 730070, China. yjma@gsau.edu.cn.
authors.forename: Youji
authors.initials: Y
authors.lastname: Ma
authors.affiliation: College of Veterinary Medicine, Gansu Agriculture University, Lanzhou 730070, China. zhangyong@gsau.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Gansu Agriculture University, Lanzhou 730070, China. zhaoxx@gsau.edu.cn.
authors.forename: Xingxu
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002417:Cattle; D003001:Cloning, Molecular; D019295:Computational Biology; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D010802:Phylogeny; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment
chemical_list: D007335:Insulin-Like Growth Factor II