title: Fluorescence in situ hybridization for identification of microorganisms in acute chorioamnionitis.
abstract: The relevance of microorganisms in preterm birth is still under discussion. Using a diagnostic fluorescence in situ hybridization probe panel, we visualized Staphylococcus aureus and Streptococcus mitis group in two cases of acute chorioamnionitis. This technique provides spatial resolution and quantity of bacteria, clarifying the epidemiology and pathogenic pathways of acute chorioamnionitis.
authors.affiliation: Klinik für Geburtsmedizin, Charité - Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Schmiedel
authors.forename: J
authors.initials: J
authors.lastname: Kikhney
authors.forename: J
authors.initials: J
authors.lastname: Masseck
authors.forename: P D
authors.initials: PD
authors.lastname: Rojas Mencias
authors.forename: J
authors.initials: J
authors.lastname: Schulze
authors.forename: A
authors.initials: A
authors.lastname: Petrich
authors.forename: A
authors.initials: A
authors.lastname: Thomas
authors.forename: W
authors.initials: W
authors.lastname: Henrich
authors.forename: A
authors.initials: A
authors.lastname: Moter
mesh_terms: D002821:Chorioamnionitis / Q000175:diagnosis* / Q000382:microbiology*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D011247:Pregnancy; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification*; D034361:Streptococcus mitis / Q000235:genetics / Q000302:isolation & purification*
keywords: Acute chorioamnionitis; biofilm; fluorescence in situ hybridization; neonatal mortality; preterm birth