title: Characterization of cytoplasmic hyaline bodies in a hepatocellular carcinoma of a dog.
abstract: This report describes the morphological and immunohistochemical features of intracytoplasmic inclusion bodies found in a 13-year-old Yorkshire dog with a hepatocellular carcinoma and referred for anorexia, lethargy and mild polydipsia. Fine-needle aspirates of the large abdominal mass revealed high number of pleomorphic neoplastic hepatocytes, containing round to polygonal, well-demarcated, hyaline bodies. Same findings were histologically confirmed on multiple biopsies. Immunohistochemically, the inclusion bodies were negative for alpha-1-antitrypsin, carcinoembryonary antigen, fibrinogen, IgG, IgM, cytokeratins 7, 8, 18, 19, 20. By transmission electron microscopy, the cytoplasmic inclusions were composed of granular homogeneous or reticulated electrondense matrix, enclosed within dilated rough endoplasmic reticulum or remnants of its membranes, consistent with proteinaceous material accumulated within neoplastic hepatocytes due to aberrant protein secretion or transport. This is the first detailed characterization of hyaline cytoplasmic inclusion bodies in canine hepatocellular carcinoma.
authors.affiliation: Veterinary Diagnostic Laboratory "San Marco", Padua, Italy. Electronic address: carlo.masserdotti@gmail.com.
authors.forename: Carlo
authors.initials: C
authors.lastname: Masserdotti
authors.affiliation: Veterinary Diagnostic Laboratory "San Marco", Padua, Italy.
authors.forename: Enrica
authors.initials: E
authors.lastname: Rossetti
authors.affiliation: Private Veterinary Clinic "San Marco", Padua, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: De Lorenzi
authors.affiliation: Department of Comparative Biomedical Sciences, Faculty of Veterinary Medicine, Teramo, Italy.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Della Salda
authors.affiliation: School of Veterinary Science, University of Queensland, Gatton campus, Gatton 4343, QLD, Australia.
authors.forename: Chiara
authors.initials: C
authors.lastname: Palmieri
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000000981:diagnostic imaging / Q000473:pathology / Q000662:veterinary*; D004283:Dog Diseases / Q000000981:diagnostic imaging / Q000473:pathology*; D004285:Dogs; D017809:Fatal Outcome; D006818:Hyalin / Q000000981:diagnostic imaging; D007150:Immunohistochemistry / Q000662:veterinary; D002479:Inclusion Bodies / Q000000981:diagnostic imaging / Q000473:pathology*; D008113:Liver Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology / Q000662:veterinary*; D008297:Male; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D014463:Ultrasonography
keywords: Dog; Hepatocellular carcinoma; Inclusion bodies; Liver