title: The use of sonication treatment to decellularize aortic tissues for preparation of bioscaffolds.
abstract: A novel decellularization method using sonication treatment is described. Sonication treatment is the combination of physical and chemical agents. These methods will disrupt cell membrane and release cell contents to external environments. The cell removal was facilitated by subsequent rinsing of sodium dodecyl sulfate detergents. Sonication treatment is used in the preparation of complete decellularized bioscaffolds. The aim of this study is to confirm the usefulness of sonication treatment for preparation of biological scaffolds. In this study, samples of aortic tissues are decellularized by sonication treatment at frequency of 170 kHz in 0.1% and 2% sodium dodecyl sulfate detergents for 10-h treatment time. The relation between decellularization and sonication parameters such as dissolved oxygen concentration, conductivity, and pH is investigated. Histological analysis and biomechanical testing is performed to evaluate cell removal efficiency as well as changes in biomechanical properties. Minimal inflammation response elicit by bioscaffolds is confirmed by xenogeneic implantation and immunohistochemistry. Sonication treatment is able to produce complete decellularized tissue suggesting that these treatments could be applied widely as one of the decellularization method.
authors.affiliation: Department of Electronic Systems Engineering, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Kuala Lumpur, Malaysia IJN-UTM Cardiovascular Engineering Centre, UTM, Johor, Malaysia The Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Hongo, Japan azhim@ic.utm.my.
authors.forename: A
authors.initials: A
authors.lastname: Azhim
authors.affiliation: Department of Electronic Systems Engineering, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Kuala Lumpur, Malaysia.
authors.forename: N
authors.initials: N
authors.lastname: Syazwani
authors.affiliation: Department of Internal Physiology Bio-Nano Medicine, National Defense Medical College, Tokorozawa, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Morimoto
authors.affiliation: Department of Bioengineering, The University of Tokyo, Hongo, Japan.
authors.forename: K S
authors.initials: KS
authors.lastname: Furukawa
authors.affiliation: Department of Internal Physiology Bio-Nano Medicine, National Defense Medical College, Tokorozawa, Japan The Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Hongo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ushida
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology* / Q000378:metabolism; D001672:Biocompatible Materials; D001696:Biomechanical Phenomena; D002469:Cell Separation; D004247:DNA / Q000302:isolation & purification; D003902:Detergents; D005109:Extracellular Matrix / Q000737:chemistry; D008422:Materials Testing; D051381:Rats; D017208:Rats, Wistar; D012967:Sodium Dodecyl Sulfate; D013010:Sonication / Q000295:instrumentation / Q000379:methods*; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014183:Transplantation, Heterologous
chemical_list: D001672:Biocompatible Materials; D003902:Detergents; D012967:Sodium Dodecyl Sulfate; D004247:DNA
keywords: Decellularization; aortic tissue; bioscaffolds; extracellular matrices; implantation; sonication