title: The effect of conditioned media of adipose-derived stem cells on wound healing after ablative fractional carbon dioxide laser resurfacing.
abstract: OBJECTIVE
  To evaluate the benefits of conditioned medium of Adipose-derived stem cells (ADSC-CM) on wound healing after fractional carbon dioxide laser resurfacing (FxCR) on human skin.
  
  
  METHODS
  Nineteen subjects were treated with FxCR on the bilateral inner arms. ADSC-CM was applied on FxCR site of one randomly selected arm. Transepidermal water loss (TEWL), skin color, and gross-elasticity of FxCR site on both arms were measured. Skin samples were taken by biopsy from three subjects 3 weeks after treatment for histopathological manifestations and mRNA expressions of procollagen types I and III, elastin genes were noted.
  
  
  RESULTS
  The index of erythema, melanin, and TEWL of the ADSC-CM-treated skin were significantly lower than those of the control side. The mRNA expression of type III procollagen in ADSC-CM-treated group at 3 weeks posttreatment was 2.6 times of that of the control group.
  
  
  CONCLUSIONS
  Application of allograft ADSC-CM is an effective method for enhancing wound healing after FxCR, by reducing transient adverse effects such as erythema, hyperpigmentation, and increased TEWL.
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Bing-Rong
authors.initials: BR
authors.lastname: Zhou
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Nanjing Regenerative Medicine Engineering and Technology Research Center, Nanjing 210029, China.
authors.forename: Shi-Lei
authors.initials: SL
authors.lastname: Guo
authors.affiliation: Nanjing Regenerative Medicine Engineering and Technology Research Center, Nanjing 210029, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Nanjing Regenerative Medicine Engineering and Technology Research Center, Nanjing 210029, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Felicia
authors.initials: F
authors.lastname: Permatasari
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Yin
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Luo
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000328:Adult; D001706:Biopsy; D017077:Culture Media, Conditioned; D005260:Female; D006801:Humans; D054020:Lasers, Gas / Q000627:therapeutic use; D008297:Male; D058629:Plasma Skin Regeneration / Q000379:methods*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D014184:Transplantation, Homologous; D014945:Wound Healing
chemical_list: D017077:Culture Media, Conditioned