title: [Expression of doublecortin-like kinase 1 in human gastric cancer and its correlation with prognosis].
abstract: OBJECTIVE
  To investigate the expression of doublecortin-like kinase 1(DCLK1)in gastric cancer and its prognostic significance.
  
  
  METHODS
  The expression of DCLK1 was examined by immunohistochemical staining of paraffin-embedded tumor specimens from 122 patients who underwent curative gastrectomy for gastric cancer at Peking Union Medical College Hospital between July 2002 and December 2006. Survival curves were described by the Kaplan-Meier method and compared by the Log-rank test. Univariate and multivariate analysis was performed with the Cox proportional hazard model.
  
  
  RESULTS
  The expression of DCLK1 in tumor cells was significantly upregulated in 51 of 122 patients. High expression of DCLK1 in tumor cells was strongly correlated with pN stage(P=0.029)and lymphovascular invasion(P=0.029). Kaplan-Meier analysis revealed that patients with DCLK1 high expression had a significantly lower 5-year overall survival(OS)rate than that of patients with DCLK1 low expression(39. 0% vs. 65. 8%, P=0.001), as well as a significantly lower 5-year disease-free survival(DFS)rate(37. 0% vs. 64. 5%, P=0.001). Univariate and multivariate analyses showed that DCLK1 expression(both P=0.036)was an independent factor for predicting OS and DFS rate.
  
  
  CONCLUSIONS
  High expression of DCLK1 in gastric cancer cells is associated with pN stage and lymphovascular invasion. It may be a predictor for poor survival in patients undergoing surgery for gastric cancer.
authors.affiliation: Department of General Surgery,PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Qing-bin
authors.initials: QB
authors.lastname: Meng
authors.affiliation: Department of General Surgery,PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Jian-chun
authors.initials: JC
authors.lastname: Yu
authors.affiliation: Department of General Surgery,PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Wei-ming
authors.initials: WM
authors.lastname: Kang
authors.affiliation: Department of General Surgery, 2Department of Pathology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Ma
authors.affiliation: Department of Pathology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Wei-xun
authors.initials: WX
authors.lastname: Zhou
authors.affiliation: Department of Pathology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of General Surgery,PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of General Surgery,PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Zhan-jiang
authors.initials: ZJ
authors.lastname: Cao
authors.affiliation: Department of General Surgery,PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Shu-bo
authors.initials: SB
authors.lastname: Tian
mesh_terms: D018572:Disease-Free Survival; D000089742:Doublecortin-Like Kinases; D005743:Gastrectomy; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D053208:Kaplan-Meier Estimate; D015999:Multivariate Analysis; D011379:Prognosis; D016016:Proportional Hazards Models; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000453:epidemiology / Q000378:metabolism*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C117347:DCLK1 protein, human; D000089742:Doublecortin-Like Kinases; D017346:Protein Serine-Threonine Kinases