title: Curcumin enhances the radiosensitivity in nasopharyngeal carcinoma cells involving the reversal of differentially expressed long non-coding RNAs.
abstract: Long non-coding RNAs (lncRNAs) are aberrantly expressed and have important functions in pathological processes. The present study investigated the lncRNA profiles and the effects of curcumin (Cur) on the radiosensitivity of nasopharyngeal carcinoma (NPC) cells. The lncRNA and mRNA profiles of each cell group were described by microarray analysis. Numerous differentially expressed genes were observed by microarrays in three cell groups. Cur significantly reversed the IR-induced lncRNA and mRNA expression signatures, shown by clustering analysis. Moreover, 116 of these IR-induced and Cur-reversed differentially expressed lncRNAs were obtained. Six lncRNAs (AF086415, AK095147, RP1-179N16.3, MUDENG, AK056098 and AK294004) were confirmed by qPCR. Furthermore, functional studies showed that lncRNA AK294004 exhibited a negative effect on cyclin D1 (CCND1), indicating that CCND1 might be a direct target of AK294004. IR-induced differentially expressed lncRNAs were reversed during Cur-enhanced radiosensitization in NPC cells, suggesting that lncRNAs have important functions in IR-induced radioresistance. Thus, Cur could serve as a good radiosensitizer.
authors.affiliation: College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, P.R. China.
authors.forename: Qirui
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, P.R. China.
authors.forename: Haoning
authors.initials: H
authors.lastname: Fan
authors.affiliation: Department of Radiotherapy, NanFang Hospital, Southern Medical University, Guangzhou 510515, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Genetic Engineering, Southern Medical University, Guangzhou 510515, P.R. China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Yin
authors.affiliation: College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, P.R. China.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Cai
authors.affiliation: Department of Radiotherapy, NanFang Hospital, Southern Medical University, Guangzhou 510515, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Radiotherapy, NanFang Hospital, Southern Medical University, Guangzhou 510515, P.R. China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, P.R. China.
authors.forename: Meng
authors.initials: M
authors.lastname: Shao
authors.affiliation: College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, P.R. China.
authors.forename: Xuegang
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, P.R. China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Diao
authors.affiliation: College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, P.R. China.
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Tong
authors.affiliation: College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, P.R. China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Fan
mesh_terms: D002277:Carcinoma; D045744:Cell Line, Tumor; D003474:Curcumin / Q000008:administration & dosage*; D019938:Cyclin D1 / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000532:radiotherapy*; D062085:RNA, Long Noncoding / Q000187:drug effects / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D011836:Radiation Tolerance / Q000187:drug effects*
chemical_list: D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D019938:Cyclin D1; D003474:Curcumin