title: Annexin A7 and its binding protein galectin-3 influence mouse hepatocellular carcinoma cell line in vitro.
abstract: Lymph node metastasis is recognized as an important mode of liver cancer metastasis. Our previous study has built two hepatocarcinoma cell lines, Hca-F with high (75%) and Hca-P with low (25%) incidences of lymph node metastasis, and has indicated that annexin A7 is an important factor in the lymphatic metastasis of tumors. There is evidence that galectin-3 is the binding protein of annexin A7 and works in protein complexes. Our current study shows that both annexin A7 and galectin-3 express higher in Hca-F than Hca-P. Annexin A7 was successfully down-regulated in Hca-P by RNA interference, and this resulted in concomitant reduction of galactin 3 expression in annexin A7 down regulated compared to the control and N-control cells. Using CCK-8 assay, the expression level of annexin A7 and galectin-3 were found to have correlation with the proliferation ability; Transwell assay showed annexin A7 and galectin-3 are involved in cell migration and invasion regulation in mouse hepatocellular carcinoma cell lines, immunofluorescence assay indicate annexin A7 and galectin-3 were co-located annexin A7 and galectin-3 played roles in DNA damage and cell proliferation cycle checkpoint arrest pathway. Those phenomena indicated that annexin A7 influences lymphatic metastasis of tumors by interacting with galectin-3 through the regulation of tumor cell proliferation, attachment, migration and invasion.
authors.affiliation: The Key laboratory of tumor metastasis in Liaoning Province, Dalian Medical University, 9 West, Lvshun Southern Road, Dalian 116044, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Song
authors.affiliation: The Key laboratory of tumor metastasis in Liaoning Province, Dalian Medical University, 9 West, Lvshun Southern Road, Dalian 116044, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Mao
authors.affiliation: The Key laboratory of tumor metastasis in Liaoning Province, Dalian Medical University, 9 West, Lvshun Southern Road, Dalian 116044, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: The Key laboratory of tumor metastasis in Liaoning Province, Dalian Medical University, 9 West, Lvshun Southern Road, Dalian 116044, China.
authors.forename: Mohammed Mohammed
authors.initials: MM
authors.lastname: Ibrahim
authors.affiliation: The Key laboratory of tumor metastasis in Liaoning Province, Dalian Medical University, 9 West, Lvshun Southern Road, Dalian 116044, China. Electronic address: lilianhong@dlmedu.edu.cn.
authors.forename: Lian-Hong
authors.initials: LH
authors.lastname: Li
authors.affiliation: The Key laboratory of tumor metastasis in Liaoning Province, Dalian Medical University, 9 West, Lvshun Southern Road, Dalian 116044, China. Electronic address: jwtang_53@sina.cn.
authors.forename: Jian-Wu
authors.initials: JW
authors.lastname: Tang
mesh_terms: D000818:Animals; D017310:Annexin A7 / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D037502:Galectin 3 / Q000096:biosynthesis* / Q000235:genetics; D008114:Liver Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D009361:Neoplasm Invasiveness; D011485:Protein Binding
chemical_list: D017310:Annexin A7; C480504:Anxa7 protein, mouse; D037502:Galectin 3; C485095:Lgals3 protein, mouse
keywords: Annexin A7; Binding protein; Galectin-3; Hepatocarcinoma; Lymphatic metastasis