title: Frequency of virulence factors in Escherichia coli isolated from suckling pigs with diarrhoea in China.
abstract: Escherichia coli-associated diarrhoea is an important disease adversely affecting the pig industry. This study was conducted to investigate the frequency of virulence factors expressed by E. coli strains isolated from suckling pigs with diarrhoea in China. A total of 381 E. coli strains, obtained from 290 faecal samples from pigs on 38 farms, were tested for fimbriae (K88, K99, 987P, F41, F18, F17), non-fimbrial adhesins (AIDA-I, paa, CS31A, eae, saa), enterotoxin (LT-I, LT-II, STa, STb, EAST1), Shiga toxin (Stx1, Stx2, Stx2e), pathogenicity islands (HPI, LEE), α-haemolysin (hlyA), afa8 gene cluster (afaD, afaE) and sepA genes by PCR. Out of the 381 isolates, 206 carried at least one virulence gene. Of the 206 virulence positive isolates, the virulence factor genes detected were EAST1 (n=120), irp2 (n=59), paa (n=50), STb (n=41), AIDA-I (n=34), LT-I (n=23), ler (n=11), hlyA (n=9), K88 (n=8), eae (n=8), STa (n=7), sepA (n=6), F18 (n=5), afaD (n=3), afaE (n=3), K99 (n=2) and Stx2e (n=1), with most isolates carrying multiple virulence genes. These results demonstrate that relatively few isolates from the study population express K88, K99, LT-I or STa, but that EAST1 (58%), irp2 (29%), AIDA-I (16.5%), paa (24%) and STb (20%) are frequent virulence factors expressed by E. coli strains isolated from suckling pigs with diarrhoea in China.
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
authors.forename: Wenxin
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
authors.forename: Chaowen
authors.initials: C
authors.lastname: Yuan
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
authors.forename: Xiangqiu
authors.initials: X
authors.lastname: Meng
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
authors.forename: Yuance
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
authors.forename: Ruize
authors.initials: R
authors.lastname: Gao
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Tang
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China. Electronic address: shidf@neau.edu.cn.
authors.forename: Dongfang
authors.initials: D
authors.lastname: Shi
mesh_terms: D000818:Animals; D000833:Animals, Suckling / Q000382:microbiology; D002681:China; D003967:Diarrhea / Q000382:microbiology* / Q000662:veterinary*; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D037521:Virulence Factors / Q000032:analysis* / Q000235:genetics
chemical_list: D037521:Virulence Factors
keywords: Diarrhoea; Escherichia coli; Suckling pigs; Virulence factors