title: Quantitative proteomic analysis of compartmentalized signaling networks.
abstract: Ras proteins operate predominantly from the plasma membrane; however, they have also been localized to most intracellular compartments. Various functions and signaling outputs have been ascribed to endomembranous Ras although systematic comparison and measurement of potential outputs have not yet been carried out. We describe the methodology for isolating and measuring compartment-specific signaling networks using quantitative proteomics. This approach reveals the potential of a subcellular platform for supporting specific outputs and will inform subsequent studies of endogenous isoform-specific Ras signaling.
authors.affiliation: Division of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom.
authors.forename: Maria
authors.initials: M
authors.lastname: Hernandez-Valladares
authors.affiliation: Division of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom.
authors.forename: Veronica
authors.initials: V
authors.lastname: Aran
authors.affiliation: Division of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom. Electronic address: iprior@liverpool.ac.uk.
authors.forename: Ian A
authors.initials: IA
authors.lastname: Prior
mesh_terms: D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D007553:Isotope Labeling; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D015398:Signal Transduction; D018631:ras Proteins / Q000378:metabolism*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D020543:Proteome; D018631:ras Proteins
keywords: FASP; Isogenic cells; Phosphopeptides; Ras; SILAC proteomics; Signaling