title: Simulation of single DNA molecule stretching and immobilization in a de-wetting two-phase flow over micropillar-patterned surface.
abstract: We investigate single DNA stretching dynamics in a de-wetting flow over micropillars using Brownian dynamics simulation. The Brownian dynamics simulation is coupled with transient flow field computation through a numerical particle tracking algorithm. The droplet formation on the top of the micropillar during the de-wetting process creates a flow pattern that allows DNA to stretch across the micropillars. It is found that DNA nanowire forms if DNA molecules could extend across the stagnation point inside the connecting water filament before its breakup. It also shows that DNA locates closer to the top wall of the micropillar has higher chance to enter the flow pattern of droplet formation and thus has higher chance to be stretched across the micropillars. Our simulation tool has the potential to become a design tool for DNA manipulation in complex biomicrofluidic devices.
authors.affiliation: Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, Ohio 43210, USA ; Center for Affordable Nanoengineering of Polymeric Biomedical Devices, 1012 Smith Laboratory, 174 W. 18th Ave., Columbus, Ohio 43210, USA.
authors.forename: Wei-Ching
authors.initials: WC
authors.lastname: Liao
authors.affiliation: Center for Affordable Nanoengineering of Polymeric Biomedical Devices, 1012 Smith Laboratory, 174 W. 18th Ave., Columbus, Ohio 43210, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Hu
authors.affiliation: Center for Affordable Nanoengineering of Polymeric Biomedical Devices, 1012 Smith Laboratory, 174 W. 18th Ave., Columbus, Ohio 43210, USA.
authors.forename: Weixiong
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, Ohio 43210, USA ; Center for Affordable Nanoengineering of Polymeric Biomedical Devices, 1012 Smith Laboratory, 174 W. 18th Ave., Columbus, Ohio 43210, USA ; Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio 43210, USA.
authors.forename: L
authors.initials: L
authors.lastname: James Lee