title: Arabidopsis WIND1 induces callus formation in rapeseed, tomato, and tobacco.
abstract: The capacity to promote cell dedifferentiation is widespread among plant species. We have recently reported that an AP2/ERF transcription factor WOUND INDUCED DEDIFFERENTIATION 1 (WIND1) and its paralogues, WIND2-4, promote cell dedifferentiation in Arabidopsis (Arabidopsis thaliana). Phylogenetic analyses suggest that AtWIND1 orthologs are found in land plants and that the shared peptide motifs between Arabidopsis paralogues are conserved in putative orthologs in dicotyledonous and monocotyledonous plants. In this study we show that AtWIND1 chemically induced rapeseed and tomato, as well as AtWIND1 constitutively expressed tobacco, promote callus formation on phytohormone-free medium. Our results suggest that the WIND1-mediated signaling cascade to promote cell dedifferentiation might be conserved in at least several species of Brassicaceae and Solanaceae.
authors.affiliation: Center for Sustainable Resource Science; RIKEN; Yokohama, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Iwase
authors.affiliation: Bioproduction Research Institute; National Institute of Advanced Industrial Science and Technology (AIST); Tsukuba, Japan.
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Mitsuda
authors.affiliation: Center for Sustainable Resource Science; RIKEN; Yokohama, Japan.
authors.forename: Momoko
authors.initials: M
authors.lastname: Ikeuchi
authors.affiliation: Center for Sustainable Resource Science; RIKEN; Yokohama, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Ohnuma
authors.affiliation: Graduate School of Agriculture; Tamagawa University; Tokyo, Japan.
authors.forename: Chie
authors.initials: C
authors.lastname: Koizuka
authors.affiliation: Gene Research Center; University of Tsukuba; Tsukuba, Japan.
authors.forename: Koich
authors.initials: K
authors.lastname: Kawamoto
authors.affiliation: Graduate School of Agriculture; Tamagawa University; Tokyo, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Imamura
authors.affiliation: Gene Research Center; University of Tsukuba; Tsukuba, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ezura
authors.affiliation: Center for Sustainable Resource Science; RIKEN; Yokohama, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Sugimoto
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D029741:Brassica rapa / Q000235:genetics / Q000502:physiology*; D017343:Genes, Plant; D018551:Solanum lycopersicum / Q000235:genetics / Q000502:physiology*; D010802:Phylogeny; D030821:Plants, Genetically Modified; D012038:Regeneration; D014026:Nicotiana / Q000235:genetics / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D014157:Transcription Factors; C559637:WIND1 protein, Arabidopsis
keywords: AP2/ERF transcription factor; WIND1; callus induction; cell dedifferentiation; tissue culture