title: Complex thiolated mannose/quinone film modified on EQCM/Au electrode for recognizing specific carbohydrate-proteins.
abstract: A complex thiolated mannose (TM)/quinone functionalised polythiophene (QFPT) thin film was modified on EQCM/Au electrode for recognition of specific carbohydrate-proteins. Different lectins such as those from Sambucus nigra (elder berry), Arachis hypogaea (peanut), Ulex europaeus (gorse, furze), Triticum vulgaris and Concanavalin A (ConA) was used for probes to evaluate bio-sensing performance of the TM/QFPT film. A specific response was observed for ConA from lectins when using the TM/QFPT film as sensing material and employing either elelctrochemical or the QCM method. No response was detected between thiolated mannose and other lectins. The linear relationship between current and ConA concentration is in the range of 0.5-17.5 nM by the elelctrochemical method and the linear relationship between frequency change and ConA concentration is in the range of 0.5-4.5 nM by the QCM method. This shows that the TM/QFPT-modified EQCM biosensor presents a paralleled determination by using electrochemical and the QCM method. The elelctrochemical method of the biosensor can be applicable in a large concentration range and its frequency change can be more precise.
authors.affiliation: State Key Laboratory of Electronic Thin Films and Integrated Devices, School of Optoelectronic Information, University of Electronic Science and Technology of China (UESTC), Chengdu 610054, P.R. China; School of Life Science and Technology, UESTC, Chengdu 610054, P.R. China. Electronic address: zeng-wang@163.com.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Electronic Thin Films and Integrated Devices, School of Optoelectronic Information, University of Electronic Science and Technology of China (UESTC), Chengdu 610054, P.R. China.
authors.forename: Junsheng
authors.initials: J
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Electronic Thin Films and Integrated Devices, School of Optoelectronic Information, University of Electronic Science and Technology of China (UESTC), Chengdu 610054, P.R. China.
authors.forename: Yadong
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Chemistry, Oakland University, Rochester, MI 48309, USA.
authors.forename: Xiangqun
authors.initials: X
authors.lastname: Zeng
mesh_terms: D016227:Benzoquinones / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008358:Mannose / Q000032:analysis* / Q000737:chemistry; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D037121:Plant Lectins / Q000032:analysis* / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013876:Thiophenes / Q000737:chemistry
chemical_list: D016227:Benzoquinones; D037121:Plant Lectins; D011108:Polymers; D013876:Thiophenes; C066730:polythiophene; C004532:quinone; D008358:Mannose
keywords: Carbohydrate–protein recognition; EQCM; Quinone-functionalised polythiophene; Thiolated mannose