title: [Determination of dicyandiamide in dairy products by high performance liquid chromatography].
abstract: A high performance liquid chromatographic (HPLC) method for the determination of dicyandiamide in dairy products was developed. The sample was extracted by acetonitrile, separated on an XBridge Amide column (250 mm x 4.6 mm, 3.5 microm) using acetonitrile-water (90/10, v/v) containing 0.2% (v/v) formic acid as the mobile phase at a flow rate of 1.0 mL/min, and determined by a photodiode array detector at the wavelength of 218 nm. The calibration curve was linear in the range of 0.5 - 50 mg/L with the correlation coefficient (r2) of 0.999 9. The recoveries were from 96.7% to 101.0% with the relative standard deviations (RSDs) in the range of 4.5% to 4.9% (n = 6). The limit of detection was 0.2 mg/kg, and the limit of quantification was 0.5 mg/kg. The method is simple, sensitive, accurate and precise for the determination of dicyandiamide in dairy products.
authors.affiliation: Zhejiang Institute of Quality Inspection Science, Key Laboratory for Food Safety of Zhejiang Province, Hangzhou 310013, China. cxz730@163.com
authors.forename: Xiaozhen
authors.initials: X
authors.lastname: Chen
authors.affiliation: Zhejiang Institute of Quality Inspection Science, Key Laboratory for Food Safety of Zhejiang Province, Hangzhou 310013, China.
authors.forename: Wanqin
authors.initials: W
authors.lastname: Chen
authors.affiliation: Zhejiang Institute of Quality Inspection Science, Key Laboratory for Food Safety of Zhejiang Province, Hangzhou 310013, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Zhejiang Institute of Quality Inspection Science, Key Laboratory for Food Safety of Zhejiang Province, Hangzhou 310013, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Huang
authors.affiliation: Zhejiang Institute of Quality Inspection Science, Key Laboratory for Food Safety of Zhejiang Province, Hangzhou 310013, China.
authors.forename: Donglei
authors.initials: D
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003611:Dairy Products / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D006146:Guanidines / Q000032:analysis*
chemical_list: D006146:Guanidines; C004711:dicyandiamido