title: Additive effect of zinc oxide nanoparticles and isoorientin on apoptosis in human hepatoma cell line.
abstract: Metal nanomaterial could effectively decrease tumour resistance to anti-cancer drugs. In this paper, we have explored the synergistic effect and mechanisms of zinc oxide nanoparticles (ZnO Nps) and isoorientin (ISO) on cytotoxicity in human hepatoma (HepG2) cells. The results showed that ZnO Nps could exert dose- and time-dependent cytotoxicity in HepG2 cells, and the combining treatment resulted in a greater cytotoxicity than single treatment. ZnO Nps could synergistically potentiate ISO to induce apoptosis through resulting in mitochondrial dysfunction, inhibiting the phosphorylation of Akt and ERK1/2, and enhancing the phosphorylation of JNK and P38. Additionally, ZnO Nps were uptaked by cells through endocytic pathway and it enhanced the cellular uptake of ISO, while no significant injury was found in normal liver cells after the combined treatment. These results suggest that the combination of metal nanoparticle with anti-cancer drugs may provide a promising alternative for novel cancer treatments.
authors.affiliation: Laboratory of Functional Chemistry and Nutrition of Food, College of Food Science and Engineering, Northwest A&F University, Yangling, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Laboratory of Functional Chemistry and Nutrition of Food, College of Food Science and Engineering, Northwest A&F University, Yangling, China.
authors.forename: Yutang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Laboratory of Functional Chemistry and Nutrition of Food, College of Food Science and Engineering, Northwest A&F University, Yangling, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Laboratory of Functional Chemistry and Nutrition of Food, College of Food Science and Engineering, Northwest A&F University, Yangling, China.
authors.forename: Haifang
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Laboratory of Functional Chemistry and Nutrition of Food, College of Food Science and Engineering, Northwest A&F University, Yangling, China. Electronic address: xueboliu@yahoo.com.cn.
authors.forename: Xuebo
authors.initials: X
authors.lastname: Liu
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation; D004357:Drug Synergism; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism; D047311:Luteolin / Q000494:pharmacology*; D020935:MAP Kinase Signaling System; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction; D015034:Zinc Oxide / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D017382:Reactive Oxygen Species; C057912:homoorientin; D000410:Alanine Transaminase; D051057:Proto-Oncogene Proteins c-akt; D047311:Luteolin; D015034:Zinc Oxide
keywords: Apoptosis; Isoorientin; MAPKs; Mitochondrial stress; PI3K/Akt; Zinc oxide nanoparticles