title: Evaluation of lovastatin effects on expression of anti-apoptotic Nrf2 and PGC-1α genes in neural stem cells treated with hydrogen peroxide.
abstract: Reactive oxygen species and oxidative stress are associated with various cell processes, including cell survival and apoptosis. Oxidative stress has been implicated in the pathogenesis of several neurological disorders including Alzheimer's disease (AD), Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS), Huntington's disease (HD), and multiple sclerosis (MS). In the present study, we evaluated the effects of lovastatin chemoprotection against hydrogen peroxide-induced oxidative stress in bone marrow stromal cell-derived neural stem cells (BMSC-derived NSCs) and whether it has protective effects. BMSC-derived NSCs were pretreated with different doses of lovastatin for 48 h and then exposed to 125 μM H2O2 for 30 min. Using MTT, TUNEL assay, and real-time RT-PCR, we evaluated the effects of lovastatin on cell survival, apoptosis, and PGC-1α and Nrf2 expression rates in pretreated BMSC-derived NSCs compared to control groups. Results showed that apoptosis rate in the lovastatin-pretreated BMSC-derived NSCs was significantly decreased compared to the control group. Our findings suggest that lovastatin protects NSCs against oxidative stress-induced cell death, and therefore, it may be used to promote the survival rate of NSCs and can be a candidate for treatment of oxidative stress-mediated neurological diseases.
authors.affiliation: Stem Cells Research Laboratory, Department of Medical Sciences, Islamic Azad University, Ardabil Branch, Ardabil, Iran, abdani.anatomy@yahoo.com.
authors.forename: Alireza
authors.initials: A
authors.lastname: Abdanipour
authors.forename: Taki
authors.initials: T
authors.lastname: Tiraihi
authors.forename: Ali
authors.initials: A
authors.lastname: Noori-Zadeh
authors.forename: Arezo
authors.initials: A
authors.lastname: Majdi
authors.forename: Ramin
authors.initials: R
authors.lastname: Gosaili
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005786:Gene Expression Regulation; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008148:Lovastatin / Q000494:pharmacology*; D051267:NF-E2-Related Factor 2 / Q000096:biosynthesis* / Q000235:genetics; D058953:Neural Stem Cells / Q000187:drug effects / Q000378:metabolism*; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D051381:Rats; D017208:Rats, Wistar; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C501429:Ppargc1a protein, rat; D014157:Transcription Factors; D008148:Lovastatin; D006861:Hydrogen Peroxide