title: Rapid immunochromatographic assay for ofloxacin in animal original foodstuffs using native antisera labeled by colloidal gold.
abstract: An immunochromatographic assay was developed to detect fluoroquinolone antibiotic ofloxacin based on the competitive binding of ofloxacin and the membrane-immobilized ofloxacin-protein conjugate to colloidal gold-labeled antibodies in the course of the labeled antibodies, and to test sample flow through the membrane. The specific feature of labeling by colloidal gold is that native antiserum is used instead of purified immunoglobulins or specific antibodies. This makes the synthetic procedure easier, with no sacrifice in the detection limit. The proposed test makes it possible to detect down to 30 ng mL(-1) of ofloxacin, which corresponds to the demands of food safety assessment. The assay time is 10 min. The assay provides reliable information on the ofloxacin content in milk without the sample preparation and in chicken and pork meat with the minimum sample preparation (the separation of the insoluble fraction of the homogenate by centrifugation). The high degree of detection of ofloxacin in foodstuffs by the proposed assay (70-112%) was shown by a comparison with the data obtained with the use of a commercial immunoenzymatic kit.
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospect 33, 119071 Moscow, Russia.
authors.forename: Nadezhda A
authors.initials: NA
authors.lastname: Byzova
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospect 33, 119071 Moscow, Russia.
authors.forename: Nataliya I
authors.initials: NI
authors.lastname: Smirnova
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospect 33, 119071 Moscow, Russia.
authors.forename: Anatoly V
authors.initials: AV
authors.lastname: Zherdev
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospect 33, 119071 Moscow, Russia; Faculty of Chemistry, M.V. Lomonosov Moscow State University, Leninskie gory 1, building 3, 119991 Moscow, Russia.
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Eremin
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospect 33, 119071 Moscow, Russia; Faculty of Chemistry, M.V. Lomonosov Moscow State University, Leninskie gory 1, building 3, 119991 Moscow, Russia.
authors.forename: Ilya A
authors.initials: IA
authors.lastname: Shanin
authors.affiliation: Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, 510642 Guangzhou, PR China.
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Lei
authors.affiliation: Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, 510642 Guangzhou, PR China. Electronic address: ymsun@scau.edu.cn.
authors.forename: Yuanming
authors.initials: Y
authors.lastname: Sun
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospect 33, 119071 Moscow, Russia. Electronic address: dzantiev@inbi.ras.ru.
authors.forename: Boris B
authors.initials: BB
authors.lastname: Dzantiev
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D002846:Chromatography, Affinity / Q000379:methods*; D003102:Colloids; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D007106:Immune Sera; D057230:Limit of Detection; D008461:Meat Products / Q000032:analysis*; D046529:Microscopy, Electron, Transmission; D015242:Ofloxacin / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000900:Anti-Bacterial Agents; D003102:Colloids; D007106:Immune Sera; D006046:Gold; D015242:Ofloxacin
keywords: Colloidal gold; Fluoroquinolones; Immunochromatographic assay; Meat testing; Milk testing; Ofloxacin