title: It's in your blood: spectral biomarker candidates for urinary bladder cancer from automated FTIR spectroscopy.
abstract: Blood samples of urinary bladder cancer (UBC) patients and patients with urinary tract infection were analysed with advanced automated high throughput Fourier transform infrared (HT-FTIR)-spectroscopy. Thin dried film samples were robotically prepared on multi-well titer plates (MTP) for absorbance measurements in transmission mode. Within the absorbance, 1st and 2nd derivative spectra of serum and two plasma preparations, discriminative patterns were identified and validated using bioinformatic tools. The optimal spectral resolution for data acquisition was determined. An accurate discrimination of the patient groups was achieved with three different independent spectral variable sets. The HT-FTIR blood test may support future clinical diagnostics.
authors.affiliation: Ruhr-Universität Bochum, Department of Biophysics ND04, Universitätsstraße 150, 44780 Bochum, Germany.
authors.forename: Julian
authors.initials: J
authors.lastname: Ollesch
authors.forename: Margot
authors.initials: M
authors.lastname: Heinze
authors.forename: H Michael
authors.initials: HM
authors.lastname: Heise
authors.forename: Thomas
authors.initials: T
authors.lastname: Behrens
authors.forename: Thomas
authors.initials: T
authors.lastname: Brüning
authors.forename: Klaus
authors.initials: K
authors.lastname: Gerwert
mesh_terms: D000368:Aged; D000465:Algorithms; D001331:Automation; D014408:Biomarkers, Tumor / Q000097:blood*; D016002:Discriminant Analysis; D006801:Humans; D008875:Middle Aged; D012371:Robotics; D012815:Signal Processing, Computer-Assisted; D013053:Spectrophotometry / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D001749:Urinary Bladder Neoplasms / Q000097:blood* / Q000175:diagnosis*; D014552:Urinary Tract Infections / Q000097:blood
chemical_list: D014408:Biomarkers, Tumor
keywords: FTIR spectroscopy; cancer biomarker; dry film blood analysis; high throughput; urinary bladder cancer