title: Size-based hydrodynamic rare tumor cell separation in curved microfluidic channels.
abstract: In this work, we propose a rapid and continuous rare tumor cell separation based on hydrodynamic effects in a label-free manner. The competition between the inertial lift force and Dean drag force inside a double spiral microchannel results in the size-based cell separation of large tumor cells and small blood cells. The mechanism of hydrodynamic separation in curved microchannel was investigated by a numerical model. Experiments with binary mixture of 5- and 15-μm-diameter polystyrene particles using the double spiral channel showed a separation purity of more than 95% at the flow rate above 30 ml/h. High throughput (2.5 × 10(8) cells/min) and efficient cell separation (more than 90%) of spiked HeLa cells and 20 × diluted blood cells was also achieved by the double spiral channel.
authors.affiliation: CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing 100190, China.
authors.forename: Jiashu
authors.initials: J
authors.lastname: Sun
authors.affiliation: LNM, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.affiliation: CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing 100190, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Li
authors.affiliation: CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing 100190, China.
authors.forename: Jidong
authors.initials: J
authors.lastname: Wang
authors.affiliation: CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing 100190, China.
authors.forename: Yunlei
authors.initials: Y
authors.lastname: Xianyu
authors.affiliation: LNM, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Hu
authors.affiliation: CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing 100190, China.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang