title: Production of mannitol from raw glycerol by Candida azyma.
abstract: To promote the effective use of raw glycerol, 13 yeast strains with the ability to produce mannitol from glycerol were isolated from environmental samples. Of the 13 strains, strain 7-12G was selected as an efficient mannitol producer from 25% (w/v) glycerol and was identified as Candida azyma by morphological, physicochemical, and phylogenetic analyses. When the ability to produce mannitol from raw glycerol in flask culture was compared among strains 7-12G, NBRC10406 (the type strain of C. azyma), and related strains, strain NBRC10406 exhibited the highest production level (31.8 g/l). Culture in jar fermentors was next investigated, and mannitol production reached 50.8 g/l over 7 days, corresponding to 0.30 g/g-glycerol. To the best of our knowledge, this is the highest reported level of mannitol produced by a microbe from glycerol under batch-type culture conditions.
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Yoshikawa
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Habe
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Tomotake
authors.initials: T
authors.lastname: Morita
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Tokuma
authors.initials: T
authors.lastname: Fukuoka
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Imura
authors.affiliation: Chemical Research Laboratories, Lion Corporation, 7-13-12 Hirai, Edogawa-ku, Tokyo 132-0035, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Iwabuchi
authors.affiliation: Chemical Research Laboratories, Lion Corporation, 7-13-12 Hirai, Edogawa-ku, Tokyo 132-0035, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Uemura
authors.affiliation: Chemical Research Laboratories, Lion Corporation, 7-13-12 Hirai, Edogawa-ku, Tokyo 132-0035, Japan.
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Tamura
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan. Electronic address: dai-kitamoto@aist.go.jp.
authors.forename: Dai
authors.initials: D
authors.lastname: Kitamoto
mesh_terms: D061249:Batch Cell Culture Techniques; D002175:Candida / Q000378:metabolism*; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D008353:Mannitol / Q000378:metabolism*; D010802:Phylogeny
chemical_list: D008353:Mannitol; D005990:Glycerol
keywords: Biodiesel; Candida azyma; Mannitol; Raw glycerol; Sugar alcohol