title: Association of levels of mannose-binding lectin and the MBL2 gene with type 2 diabetes and diabetic nephropathy.
abstract: OBJECTIVE
  To investigate the association of Mannose-binding lectin (MBL) and the MBL2 gene with type 2 diabetes and diabetic nephropathy and the influence of MBL2 polymorphisms on serum MBL levels.
  
  
  METHODS
  The study population included 675 type 2 diabetic patients with or without nephropathy and 855 normoglycemic controls. The single nucleotide polymorphisms (SNPs) of rs1800450, rs1800451, and rs11003125 of the MBL2 gene were determined by the Multiplex Snapshot method. Serum MBL levels were measured by enzyme-linked immune sorbent assay.
  
  
  RESULTS
  Rs1800450 and rs11003125 SNPs demonstrated strong linkage disequilibrium in the study population (r(2) = 0.97). The haplotypes constructed from the G allele of rs1800450 and the C allele of rs11003125 increased the risk for type 2 diabetes (OR = 1.2, 95% CI = 1.1-1.4, P = 0.01). For rs1800450, GG and GA genotypes were associated with type 2 diabetes (P = 0.02, 0.01, respectively). For rs11003125, the GC genotype frequency was significantly different between patients and controls (18.1% vs. 24.9%, P = 0.001). Analyses of genotypes and allele frequency distributions among patients with normal UAE, microalbuminuria, and macroalbuminuria showed that there was no obvious evidence of association between the MBL2 gene and diabetic nephropathy. Subjects with the GG genotype of rs1800450 and the CC genotype of rs11003125 had much higher serum MBL levels.
  
  
  CONCLUSIONS
  The rs1800450 and rs11003125 SNPs of the MBL2 gene have strong linkage disequilibrium and are associated with type 2 diabetes in the North Chinese Han population. No association was observed between the MBL2 gene and diabetic nephropathy. Subjects with the GG genotype of rs1800450 and the CC genotype of rs11003125 had much higher serum MBL levels. An association between elevated serum MBL and diabetic nephropathy was also observed.
authors.affiliation: Clinical Epidemiology Unit, Qilu Hospital of Shandong University, Jinan, Shandong, P. R. China.
authors.forename: Nana
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Clinical Epidemiology Unit, Qilu Hospital of Shandong University, Jinan, Shandong, P. R. China.
authors.forename: Maoqiang
authors.initials: M
authors.lastname: Zhuang
authors.affiliation: Clinical Epidemiology Unit, Qilu Hospital of Shandong University, Jinan, Shandong, P. R. China.
authors.forename: Aixia
authors.initials: A
authors.lastname: Ma
authors.affiliation: Clinical Epidemiology Unit, Qilu Hospital of Shandong University, Jinan, Shandong, P. R. China.
authors.forename: Guochang
authors.initials: G
authors.lastname: Wang
authors.affiliation: Clinical Epidemiology Unit, Qilu Hospital of Shandong University, Jinan, Shandong, P. R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Cheng
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes of Biomedical Sciences, Fudan University, Shanghai, P. R. China ; CMC Institute of Health Sciences, Taizhou, Jiangsu, P. R. China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Yang
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes of Biomedical Sciences, Fudan University, Shanghai, P. R. China ; CMC Institute of Health Sciences, Taizhou, Jiangsu, P. R. China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: CMC Institute of Health Sciences, Taizhou, Jiangsu, P. R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes of Biomedical Sciences, Fudan University, Shanghai, P. R. China ; CMC Institute of Health Sciences, Taizhou, Jiangsu, P. R. China.
authors.forename: Xingdong
authors.initials: X
authors.lastname: Chen
authors.affiliation: Clinical Epidemiology Unit, Qilu Hospital of Shandong University, Jinan, Shandong, P. R. China ; MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences and Institutes of Biomedical Sciences, Fudan University, Shanghai, P. R. China ; CMC Institute of Health Sciences, Taizhou, Jiangsu, P. R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Lu
mesh_terms: D000368:Aged; D000483:Alleles; D044466:Asian People; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D003928:Diabetic Nephropathies / Q000097:blood / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D037601:Mannose-Binding Lectin / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin