title: Establishment and partial characterization of a human tumor cell line, GBM-HSF, from a glioblastoma multiforme.
abstract: This paper outlines the establishment of a new and stable cell line, designated GBM-HSF, from a malignant glioblastoma multiforme (GBM) removed from a 65-year-old Chinese woman. This cell line has been grown for 1 year without disruption and has been passaged over 50 times. The cells were adherently cultured in RPMI-1640 media with 10% fetal bovine serum supplementation. Cells displayed spindle and polygonal morphology, and displayed multi-layered growth without evidence of contact inhibition. The cell line had a high growth rate with a doubling time of 51 h. The cells were able to grow without adhering to the culture plates, and 4.5% of the total cells formed colonies in soft agar. The cell line has also been found to form tumors in nude mice and to be of a highly invasive nature. The cells were also partially characterized with RT-PCR. The RT-PCR revealed that Nestin, β-tubulin III, Map2, Klf4, Oct4, Sox2, Nanog, and CD26 were positively transcribed, whereas GFAP, Rex1, and CD133 were negatively transcribed in this cell line. These results suggest that the GBM-HSF cell line will provide a good model to study the properties of cancer stem cells and metastasis. It will also facilitate more detailed molecular and cellular studies of GBM cell division and pathology.
authors.affiliation: University of Science and Technology of China, Hefei, Anhui, 230026, People's Republic of China, shark2004@sina.com.
authors.forename: Jiagui
authors.initials: J
authors.lastname: Qu
authors.forename: Joshua D
authors.initials: JD
authors.lastname: Rizak
authors.forename: Yaodong
authors.initials: Y
authors.lastname: Fan
authors.forename: Xiaoxuan
authors.initials: X
authors.lastname: Guo
authors.forename: Jiejing
authors.initials: J
authors.lastname: Li
authors.forename: Tanzeel
authors.initials: T
authors.lastname: Huma
authors.forename: Yuanye
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000368:Aged; D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D002455:Cell Division / Q000235:genetics; D005260:Female; D005909:Glioblastoma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism; D008819:Mice, Nude; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D014411:Neoplastic Stem Cells / Q000473:pathology; D064231:Nestin / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D017397:Prefrontal Cortex; D055748:SOXB1 Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics; D014404:Tubulin / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: C000715467:KLF4 protein, human; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; C489788:MAP2 protein, human; D008869:Microtubule-Associated Proteins; D064231:Nestin; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D014404:Tubulin