title: Ultrasound/microwave-assisted extraction and comparative analysis of bioactive/toxic indole alkaloids in different medicinal parts of Gelsemium elegans Benth by ultra-high performance liquid chromatography with MS/MS.
abstract: Indole alkaloids are the main bioactive/toxic components in Gelsemium elegans Benth. To determine the distribution and contents of indole alkaloids in its different medicinal parts, a novel and rapid method using ultra-high performance LC (UPLC) with MS/MS has been established and validated with an optimized ultrasound/microwave-assisted extraction method. Four constituents, namely, humantenidine, humantenmine, gelsemine, and koumine, were simultaneously determined in 6 min. Chromatographic separation was achieved on an ultra-high performance LC BEH C18 column with a gradient mobile phase consisting of methanol and water (containing 0.1% formic acid both in methanol and water) at a flow rate of 0.3 mL/min. The detection was performed on a triple quadrupole electrospray MS/MS by positive ion multiple-reaction monitoring mode. All the analytes showed good linearity (r ≥ 0.9934) within a concentration range from 0.1-25 μg/mL with a LOQ of 25-50 ng/mL. The overall intra- and intervariations of four components were <4.7% with an accuracy of 97.3-101.3%. The analysis results showed that there were remarkable differences in the distribution and contents of four chemical markers in the roots, stems, and leaves of G. elegans Benth. The findings can provide necessary and meaningful information for the rational utilization of its resources.
authors.affiliation: School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Rong-Jie
authors.initials: RJ
authors.lastname: Zeng
authors.forename: Qing
authors.initials: Q
authors.lastname: Lu
authors.forename: Shui-Sheng
authors.initials: SS
authors.lastname: Wu
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Chen
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D031450:Gelsemium / Q000737:chemistry*; D026121:Indole Alkaloids / Q000737:chemistry* / Q000302:isolation & purification* / Q000633:toxicity; D008872:Microwaves; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014465:Ultrasonics / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D026121:Indole Alkaloids
keywords: Comparative analysis; G. elegans Benth; Indole alkaloids; Medicinal plants