title: A fluorogenic probe with aggregation-induced emission characteristics for carboxylesterase assay through formation of supramolecular microfibers.
abstract: Herein, we report a novel fluorescent "light-up" probe useful for carboxylesterase assay that is based on a tetraphenylethylene derivative containing carboxylic ester groups. The specific cleavage of the carboxylic ester bonds by carboxylesterase results in the generation of a relatively hydrophobic moiety that self-assembles into supramolecular microfibers, thus giving rise to "turn-on" fluorescent signals. A high sensitivity towards carboxylesterase was achieved with a detection limit as low as 29 pM, which is much lower than the corresponding assays based on other fluorescent approaches.
authors.affiliation: College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, 215123 (China), Tel: (+86) 0512-65882917.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Wang
authors.forename: Huan
authors.initials: H
authors.lastname: Liu
authors.forename: Jingwen
authors.initials: J
authors.lastname: Li
authors.forename: Kaiguo
authors.initials: K
authors.lastname: Ding
authors.forename: Zhonglin
authors.initials: Z
authors.lastname: Lv
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Yang
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Xinming
authors.initials: X
authors.lastname: Li
mesh_terms: D043182:Carboxylesterase / Q000032:analysis* / Q000378:metabolism*; D057075:Enzyme Assays; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D046911:Macromolecular Substances / Q000737:chemistry; D015394:Molecular Structure; D010316:Particle Size; D013499:Surface Properties
chemical_list: D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D043182:Carboxylesterase
keywords: aggregation-induced emission; carboxylesterase; fluorescence; self-assembly; tetraphenylethylene