title: Antigen expression patterns of MYC-rearranged versus non-MYC-rearranged B-cell lymphomas by flow cytometry.
abstract: B-cell lymphomas with MYC rearrangement are a heterogeneous and unique group of neoplasms that may behave aggressively, especially in conjunction with other oncogenic alterations that cooperate with MYC, warranting early and intensive treatment in a subset of patients. Identification of MYC-rearranged B-cell lymphomas by immunophenotypic analysis could help guide cytogenetic work-up, as well as expedite therapeutic interventions. Using flow cytometry we analyzed the expression of CD10, CD19, CD20, CD38 and CD45 in 53 patients with three distinct neoplasms with MYC rearrangements: Burkitt lymphoma (n = 12), double hit lymphoma (n = 17) and diffuse large B-cell lymphoma (DLBCL) with MYC rearrangement (n = 8). The expression profile of these commonly used antibodies was similar among these three groups. The antigenic pattern of bright CD38 and dim CD45 was unique to the MYC-rearranged neoplasms when compared to non-MYC-rearranged aggressive DLBCL (n = 16), with a combined sensitivity of 67% and specificity of 100%, suggesting a shared underlying biology among these MYC rearranged B-cell lymphomas.
authors.affiliation: Department of Pathology, Brigham and Women's Hospital , Boston, MA , USA.
authors.forename: Diana L
authors.initials: DL
authors.lastname: Mandelker
authors.forename: David M
authors.initials: DM
authors.lastname: Dorfman
authors.forename: Betty
authors.initials: B
authors.lastname: Li
authors.forename: Olga
authors.initials: O
authors.lastname: Pozdnyakova
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism*; D018941:Antigens, CD19 / Q000378:metabolism; D018951:Antigens, CD20 / Q000378:metabolism; D002051:Burkitt Lymphoma / Q000235:genetics / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods*; D015321:Gene Rearrangement; D006801:Humans; D016130:Immunophenotyping; D017493:Leukocyte Common Antigens / Q000378:metabolism; D016393:Lymphoma, B-Cell / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics / Q000378:metabolism; D015260:Neprilysin / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*
chemical_list: D015703:Antigens, CD; D018941:Antigens, CD19; D018951:Antigens, CD20; D016271:Proto-Oncogene Proteins c-myc; D017493:Leukocyte Common Antigens; D051997:ADP-ribosyl Cyclase 1; D015260:Neprilysin
keywords: CD38; CD45; Expression pattern; MYC rearrangement; aggressive B-cell lymphoma