title: Molecular imaging detects impairment in the retrograde axonal transport mechanism after radiation-induced spinal cord injury.
abstract: OBJECTIVE
  The goal of this study was to determine whether molecular imaging of retrograde axonal transport is a suitable technique to detect changes in the spinal cord in response to radiation injury.
  
  
  METHODS
  The lower thoracic spinal cords of adult female BALB/c mice were irradiated with single doses of 2, 10, or 80 Gy. An optical imaging method was used to observe the migration of the fluorescently labeled nontoxic C-fragment of tetanus toxin (TTc) from an injection site in the calf muscles to the spinal cord. Changes in migration patterns compared with baseline and controls allowed assessment of radiation-induced alterations in the retrograde neuronal axonal transport mechanism. Subsequently, tissues were harvested and histological examination of the spinal cords performed.
  
  
  RESULTS
  Transport of TTc in the thoracic spinal cord was impaired in a dose-dependent manner. Transport was significantly decreased by 16 days in animals exposed to either 10 or 80 Gy, while animals exposed to 2 Gy were affected only minimally. Further, animals exposed to the highest dose also experienced significant weight loss by 9 days and developed posterior paralysis by 45 days. Marked histological changes including vacuolization, and white matter necrosis were observed in radiated cords after 30 days for mice exposed to 80 Gy.
  
  
  CONCLUSIONS
  Radiation of the spinal cord induces dose-dependent changes in retrograde axonal transport, which can be monitored by molecular imaging. This approach suggests a novel diagnostic modality to assess nerve injury and monitor therapeutic interventions.
authors.affiliation: Department of Cancer Systems Imaging, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: Lucia G
authors.initials: LG
authors.lastname: LeRoux
authors.forename: Sebastian
authors.initials: S
authors.lastname: Bredow
authors.forename: David
authors.initials: D
authors.lastname: Grosshans
authors.forename: Dawid
authors.initials: D
authors.lastname: Schellingerhout
mesh_terms: D000818:Animals; D001370:Axonal Transport; D005260:Female; D008807:Mice, Inbred BALB C; D057054:Molecular Imaging / Q000379:methods*; D011832:Radiation Injuries / Q000175:diagnosis* / Q000473:pathology*; D013116:Spinal Cord / Q000473:pathology / Q000528:radiation effects; D013119:Spinal Cord Injuries / Q000175:diagnosis* / Q000473:pathology*