title: Risk factors and outcome analysis of acinetobacter baumannii complex bacteremia in critical patients.
abstract: OBJECTIVE
  Acinetobacter baumannii complex bacteremia has been identified increasingly in critical patients admitted in ICUs. Notably, A. baumannii complex bacteremia has a high mortality rate, yet the risk factors associated with mortality remain unclear and controversial.
  
  
  METHODS
  Retrospective study.
  
  
  METHODS
  All adult ICUs at a tertiary care medical center.
  
  
  METHODS
  All patients with A. baumannii complex bacteremia admitted in 2009-2010.
  
  
  METHODS
  None.
  
  
  RESULTS
  Risk factors for mortality were analyzed. Bacterial isolates were identified by 16S-23S ribosomal RNA intergenic spacer region sequencing for genospecies and genotyped by pulsed-field gel electrophoresis. Carbapenemase genes were detected by polymerase chain reaction and sequencing. A total of 298 patients met the inclusion criteria, including 73 (24.5%) infected by imipenem-resistant A. baumannii complex. The overall 30-day mortality was 33.6% (100 of 298). Imipenem-resistant A. baumannii complex bacteremia specifically showed a high mortality (69.9%) and was associated with prior use of broad-spectrum antibiotics for more than 5 days for treating ventilator-associated pneumonia before the occurrence of bacteremia. Mortality was associated with inappropriate initial antimicrobial therapy, which was correlated with imipenem-resistant A. baumannii complex but not with any specific genospecies. ISAba1-blaOXA-23-ISAba1 (Tn2006) was found in most (66.7%, 40 of 68) imipenem-resistant A. baumannii (genospecies 2) and also spread beyond species border to all imipenem-resistant genospecies 3 (2), 13TU (2), and 10 (1).
  
  
  CONCLUSIONS
  For critical patients with A. baumannii complex infection, ventilator-associated pneumonia in particular, the selective pressure from prior use of broad-spectrum antibiotics for 5 days or more increased risk of subsequent imipenem-resistant A. baumannii complex bacteremia. To reduce mortality, rapid identification of imipenem-resistant A. baumannii complex and early initiation of appropriate antimicrobial therapy in these high-risk patients are crucial.
authors.affiliation: 1Graduate Institute of Clinical Medical Sciences, Chang Gung University College of Medicine, Taoyuan, Taiwan. 2Department of Pediatrics, Chang Gung Children's Hospital, Chang Gung University College of Medicine, Taoyuan, Taiwan. 3Molecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taoyuan, Taiwan.
authors.forename: Hao-Yuan
authors.initials: HY
authors.lastname: Lee
authors.forename: Chyi-Liang
authors.initials: CL
authors.lastname: Chen
authors.forename: Si-Ru
authors.initials: SR
authors.lastname: Wu
authors.forename: Chih-Wei
authors.initials: CW
authors.lastname: Huang
authors.forename: Cheng-Hsun
authors.initials: CH
authors.lastname: Chiu
mesh_terms: D000151:Acinetobacter Infections / Q000188:drug therapy / Q000401:mortality*; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D016470:Bacteremia / Q000382:microbiology* / Q000401:mortality; D001426:Bacterial Proteins / Q000235:genetics*; D016638:Critical Illness / Q000401:mortality; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D015378:Imipenem / Q000627:therapeutic use*; D007362:Intensive Care Units; D053208:Kaplan-Meier Estimate; D008297:Male; D015999:Multivariate Analysis; D053717:Pneumonia, Ventilator-Associated / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S; D012189:Retrospective Studies; D012307:Risk Factors; D062606:Tertiary Care Centers; D016896:Treatment Outcome; D018440:beta-Lactam Resistance / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D015378:Imipenem