title: Processing technique for lipofilling influences adipose-derived stem cell concentration and cell viability in lipoaspirate.
abstract: BACKGROUND
  Autologous fat grafting is a highly used technique in plastic and reconstructive surgery. Several fat-processing techniques have been described, with centrifugation frequently touted as the optimal method. Processing is one factor important for maximizing cell viability and adipose-derived mesenchymal stem cell (ADSC) concentrations. This study compared two methods of fat preparation (centrifugation vs Telfa-rolling) to determine which technique results in the greatest degree of cell viability and ADSC concentration.
  
  
  METHODS
  Abdominal fat was harvested from five patients. Equal aliquots were divided and processed by both centrifugation and Telfa-rolling. Samples were analyzed for ADSC proportions via flow cytometry and cell viability using methylene blue-based cell counting. Paired t tests were performed on all samples, and a P value lower than 0.05 was considered statistically significant.
  
  
  RESULTS
  Telfa-rolling processing resulted in a higher percentage of isolated ADSCs (P < 0.5 for 3 of 4 parameters) and a significantly higher number of viable cells (P < 0.05).
  
  
  CONCLUSIONS
  Telfa-rolling results in a higher proportion of ADSCs and greater cell viability than centrifugation for donor adipose graft preparation. Further studies are necessary to confirm whether optimal preparation translates to improved augmentation and cell take at the recipient site.
  
  
  METHODS
  This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
authors.affiliation: Section of Plastic and Reconstructive Surgery, Yale University School of Medicine, 3rd Floor, Boardman Building, 330 Cedar Street, New Haven, CT, 06520, USA.
authors.forename: Miles
authors.initials: M
authors.lastname: Pfaff
authors.affiliation: Section of Plastic and Reconstructive Surgery, Yale University School of Medicine, 3rd Floor, Boardman Building, 330 Cedar Street, New Haven, CT, 06520, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Section of Plastic and Reconstructive Surgery, Yale University School of Medicine, 3rd Floor, Boardman Building, 330 Cedar Street, New Haven, CT, 06520, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Zellner
authors.affiliation: Section of Plastic and Reconstructive Surgery, Yale University School of Medicine, 3rd Floor, Boardman Building, 330 Cedar Street, New Haven, CT, 06520, USA. derek.steinbacher@yale.edu.
authors.forename: Derek M
authors.initials: DM
authors.lastname: Steinbacher
mesh_terms: D017667:Adipocytes; D002452:Cell Count; D002470:Cell Survival; D002498:Centrifugation; D006801:Humans; D015187:Lipectomy; D013234:Stem Cells