title: Comparison of plasma and erythrocyte membrane fatty acid compositions in patients with end-stage renal disease and type 2 diabetes mellitus.
abstract: BACKGROUND
  In this study, we aimed to compare the serum lipid profile and fatty acid (FA) compositions of erythrocyte membrane (EM) and plasma in three different patient groups (group 1: type 2 diabetes mellitus (T2DM)+end-stage renal disease (ESRD), group 2: ESRD, group 4: T2DM) and healthy controls (group 3) simultaneously.
  
  
  METHODS
  40 ESRD patients treated with hemodialysis (HD) in Gulhane School of Medicine (20 with T2DM) and 32 controls (17 with T2DM, 15 healthy controls) were included in the study. Plasma and EM FA concentrations were measured by gas chromatography-flame ionization detector (GC-FID).
  
  
  RESULTS
  Plasma and EM palmitic acid (PA) and stearic acid (SA) levels were significantly higher in T2DM patients compared to controls (p=0.040 and p=0.002 for plasma, p=0.001 and p=0.010 for EM, respectively). EM docosahexaenoic acid (DHA) levels were also significantly lower in patients with ESRD+T2DM and ESRD compared to controls (p=0.004 and p=0.037, respectively).
  
  
  CONCLUSIONS
  Patients with insulin resistance display a pattern of high long chain saturated FAs (PA, SA and arachidic acids). However, while there are no recognized standards for normal EM DHA content, decreased levels of EM DHA in ESRD patient groups (groups 1 and 2) suggest that there may be reduced endogenous synthesis of DHA in HD subjects, due to the decreased functionality of desaturase and elongase enzymes. Because membrane PUFA content affects membrane fluidity and cell signaling, these findings are worthy of further investigation.
authors.affiliation: Ankara Mevki Military Hospital, Anittepe Dispensary, Ankara, Turkey. Electronic address: erdimsertoglu@gmail.com.
authors.forename: Erdim
authors.initials: E
authors.lastname: Sertoglu
authors.affiliation: Gulhane School of Medicine, Department of Medical Biochemistry, Ankara, Turkey.
authors.forename: Ismail
authors.initials: I
authors.lastname: Kurt
authors.affiliation: Gulhane School of Medicine, Department of Medical Biochemistry, Ankara, Turkey.
authors.forename: Serkan
authors.initials: S
authors.lastname: Tapan
authors.affiliation: Gulhane School of Medicine, Department of Medical Biochemistry, Ankara, Turkey.
authors.forename: Metin
authors.initials: M
authors.lastname: Uyanik
authors.affiliation: Acıbadem University School of Medicine, Department of Medical Biochemistry, Istanbul, Turkey.
authors.forename: Muhittin A
authors.initials: MA
authors.lastname: Serdar
authors.affiliation: Adana Military Hospital, Department of Medical Biochemistry, Adana, Turkey.
authors.forename: Huseyin
authors.initials: H
authors.lastname: Kayadibi
authors.affiliation: Gulhane School of Medicine, Department of Medical Biochemistry, Ankara, Turkey.
authors.forename: Saad
authors.initials: S
authors.lastname: El-Fawaeir
mesh_terms: D000328:Adult; D002462:Cell Membrane / Q000737:chemistry*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism* / Q000473:pathology; D004281:Docosahexaenoic Acids / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000737:chemistry*; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D019308:Palmitic Acid / Q000378:metabolism; D013229:Stearic Acids / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D013229:Stearic Acids; D004281:Docosahexaenoic Acids; D019308:Palmitic Acid; C031183:stearic acid
keywords: End-stage renal disease; Erythrocyte membrane; Fatty acids; Plasma; Type 2 diabetes mellitus