title: Methylation of SOCS3 in Myeloproliferative Neoplasms and Secondary Erythrocytosis/Thrombocythemia.
abstract: OBJECTIVE
  Myeloproliferative neoplasms (MPNs) like essential thrombocythemia (ET), polycythemia vera (PV), and primary myelofibrosis (PMF) are acquired clonal hematopoietic stem cell disorders and originate from a multipotent hematopoietic stem cell. The SOCS1 and SOCS3 genes are negative regulators of the JAK/STAT signal pathway. In this study we investigate the promoter methylation of these genes in the pathogenesis of MPNs and secondary erythrocytosis/thrombocythemia.
  
  
  METHODS
  Promoter methylation of SOCS1 and SOCS3 genes was analyzed with methylation-specific PCR. PCR products were analyzed by agarose gel electrophoresis.
  
  
  RESULTS
  No disease-specific CpG island methylation of SOCS1 was observed. Hypermethylation of the SOCS3 promoter was identified in 5 out of 19 (26.3%) PV cases, 2 out of 21 (9.5%) ET cases, 1 out of 5 (20%) PMF cases, and 9 out of 42 (21.4%) cases of secondary erythrocytosis/thrombocythemia.
  
  
  CONCLUSIONS
  The results revealed that promoter methylation of the SOCS3 gene suggests a possible role for SOCS3 methylation in the pathogenesis of MPNs and secondary erythrocytosis/thrombocythemia.
  
  
  BACKGROUND
  None declared.
authors.affiliation: Gülhane Military Medical Faculty, Department of Medical Genetics, Ankara, Turkey.
authors.forename: Deniz
authors.initials: D
authors.lastname: Torun
authors.affiliation: Gülhane Military Medical Faculty, Department of Hematology, Ankara, Turkey.
authors.forename: Oral
authors.initials: O
authors.lastname: Nevruz
authors.affiliation: Gülhane Military Medical Faculty, Department of Biostatistics, Ankara, Turkey.
authors.forename: Mesut
authors.initials: M
authors.lastname: Akyol
authors.affiliation: Gülhane Military Medical Faculty, Department of Medical Genetics, Ankara, Turkey.
authors.forename: Salih
authors.initials: S
authors.lastname: Kozan
authors.affiliation: Gülhane Military Medical Faculty, Department of Medical Genetics, Ankara, Turkey.
authors.forename: Muhterem
authors.initials: M
authors.lastname: Bahçe
authors.affiliation: Gülhane Military Medical Faculty, Department of Medical Biology, Ankara, Turkey.
authors.forename: Sefik
authors.initials: S
authors.lastname: Güran
authors.affiliation: Gülhane Military Medical Faculty, Department of Hematology, Ankara, Turkey.
authors.forename: Cengiz
authors.initials: C
authors.lastname: Beyan
keywords: Myeloproliferative neoplasm; SOCS1; SOCS3; Secondary erythrocytosis/thrombocythemia