title: [Preliminary screening of activity fraction of Alchornea trewioides suppresses expression of subgenomic hepatitis C virus RNA in vitro].
abstract: OBJECTIVE
  To screen activity fraction of Alchornea trewioides which suppresses expression of subgenomic Hepatitis C Virus (HCV) RNA in vitro.
  
  
  METHODS
  Anti-HCV effects in vitro were examined in an HCV subgenomic replicon cell culture system--CBRH7919 (Jneo3-5B). The cells were exposed to different concentrations of A. trewioides initial ethanol extracts, portions of petroleum ether,ethyl acetate and n-butanol extracts with interferon a combined with ribavirin as positive control. The content of HCV RNA was examined by Quantitative PCR. The expression levels of functional proteins NS3 were examined in all groups by Western blot. Cell proliferation test with CCK-8 assay was used to evaluate the cytotoxicity of drugs.
  
  
  RESULTS
  The study showed that exposure of CBRH7919 (Jneo3-5B) cells to ethyl acetate extract of A. trewioides resulted in a concentration-dependent inhibition of subgenomic HCV RNA replication and NS3 protein expression ability among the four extracts (P < 0.05). The activity of ethyl acetate extract was increased by 5.71 times than that of the initial ethanol extract. IC50 to subgenomic HCV RNA was 14.60 mg/L, CC50 to CBRH7919 (Jneo3-5B) cells was 40.30 mg/L and the treatment index (TI) was 2. 76.
  
  
  CONCLUSIONS
  The ethyl acetate extract of A. trewioides is the activity fraction which can significantly interfere with subgenomic HCV RNA replication and expression of NS3 protein in vitro. These data suggest that ethyl acetate extract isolated from A. trewioides may have potential use as an anti-HCV compound.
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China. wqrv@hotmail.com
authors.forename: Qi-Rui
authors.initials: QR
authors.lastname: Wang
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Hua-Ming
authors.initials: HM
authors.lastname: Tao
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Fan
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Shao-Hui
authors.initials: SH
authors.lastname: Huang
authors.affiliation: People's Hospital of Boluo County, Huizhou 516100, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Hong
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Lv
mesh_terms: D000085:Acetates; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D019665:Euphorbiaceae / Q000737:chemistry*; D016174:Hepacivirus / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D020128:Inhibitory Concentration 50; D016898:Interferon-alpha / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D011480:Protease Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D012367:RNA, Viral / Q000187:drug effects; D012254:Ribavirin / Q000494:pharmacology; D017361:Viral Nonstructural Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D000085:Acetates; D000998:Antiviral Agents; D016898:Interferon-alpha; D010936:Plant Extracts; D011480:Protease Inhibitors; D012367:RNA, Viral; D012254:Ribavirin; D017361:Viral Nonstructural Proteins; C084422:NS3 protein, hepatitis C virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases; C007650:ethyl acetate