title: Fingerprinting DNA oxidation processes: IR characterization of the 5-methyl-2'-deoxycytidine radical cation.
abstract: Methylated cytidine plays an important role as an epigenetic signal in gene regulation. Its oxidation products are assumed to be involved in active demethylation processes but also in damaging DNA. Here, we report the photochemical production of the 5-methyl-2'-deoxycytidine radical cation via a two-photon ionization process. The radical cation is detected by time-resolved IR spectroscopy and identified by band assignment using density functional theory calculations. Two final oxidation products are characterized with liquid chromatography coupled to mass spectrometry.
authors.affiliation: BioMolecular Optics and Center for Integrated Protein Science, Ludwig-Maximilians-Universität, Oettingenstr. 67, 80538 München (Germany), Fax: (+49) 89-2180-9202; Center for Integrated Protein Science, Department für Chemie, Ludwig-Maximilians-Universität, Butenandtstr. 5-13, 81377 München (Germany).
authors.forename: Dominik B
authors.initials: DB
authors.lastname: Bucher
authors.forename: Bert M
authors.initials: BM
authors.lastname: Pilles
authors.forename: Toni
authors.initials: T
authors.lastname: Pfaffeneder
authors.forename: Thomas
authors.initials: T
authors.lastname: Carell
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Zinth
mesh_terms: D002412:Cations / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry*; D010084:Oxidation-Reduction; D013055:Spectrophotometry, Infrared
chemical_list: D002412:Cations; D005609:Free Radicals; D003841:Deoxycytidine; D004247:DNA; C016569:5-methyldeoxycytidine
keywords: 5-methyl-2′-deoxycytidine; DNA damage; DNA oxidation; time-resolved spectroscopy; vibrational spectroscopy