title: Comparison of the performance of the Cepheid Xpert HemosIL Factor II and Factor V and the ViennaLab FV-PTH-MTHFR StripAssay kits for molecular thrombophilia profiling.
abstract: OBJECTIVE
  To compare the performance of two assays used for the detection of mutations/polymorphisms in the Factor V, Factor II, and methylenetetrahydrofolate reductase genes among patients referred for the management of a thrombotic event.
  
  
  METHODS
  We tested 40 different patient samples using two assays, the ViennaLab FV-PTH-MTHFR StripAssay and the Cepheid Xpert HemosIL.
  
  
  RESULTS
  The two assays were 100% concordant in their produced results with no samples failing the testing procedures in both.
  
  
  CONCLUSIONS
  This is the first report to evaluate the performance of the ViennaLab FV-PTH-MTHFR StripAssay and the Cepheid Xpert HemosIL. Both assays can be introduced to the operation of molecular diagnostic laboratories to cover the referrals from different disciplines, especially in tertiary care centers with emergency departments.
authors.affiliation: Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center , Beirut, Lebanon .
authors.forename: Rouba
authors.initials: R
authors.lastname: Hoteit
authors.forename: Sylvana
authors.initials: S
authors.lastname: Hassanieh
authors.forename: Rami A
authors.initials: RA
authors.lastname: Mahfouz
mesh_terms: D004252:DNA Mutational Analysis / Q000379:methods*; D005165:Factor V / Q000032:analysis / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D060005:Genotyping Techniques / Q000379:methods; D006801:Humans; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000032:analysis / Q000235:genetics*; D025202:Molecular Diagnostic Techniques / Q000379:methods; D011110:Polymorphism, Genetic; D011516:Prothrombin / Q000032:analysis / Q000235:genetics*; D011933:Reagent Kits, Diagnostic; D011934:Reagent Strips; D019851:Thrombophilia / Q000175:diagnosis* / Q000235:genetics
chemical_list: D011933:Reagent Kits, Diagnostic; D011934:Reagent Strips; D005165:Factor V; D011516:Prothrombin; C573423:MTHFR protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2)