title: Role of NADH: quinone oxidoreductase-1 in the tight junctions of colonic epithelial cells.
abstract: NADH:quinone oxidoreductase 1 (NQO1) is known to be involved in the regulation of energy synthesis and metabolism, and the functional studies of NQO1 have largely focused on metabolic disorders. Here, we show for the first time that compared to NQO1-WT mice, NQO1-KO mice exhibited a marked increase of permeability and spontaneous inflammation in the gut. In the DSS-induced colitis model, NQO1-KO mice showed more severe inflammatory responses than NQO1-WT mice. Interestingly, the transcript levels of claudin and occludin, the major tight junction molecules of gut epithelial cells, were significantly decreased in NQO1-KO mice. The colons of NQO1-KO mice also showed high levels of reactive oxygen species (ROS) and histone deacetylase (HDAC) activity, which are known to affect transcriptional regulation. Taken together, these novel findings indicate that NQO1 contributes to the barrier function of gut epithelial cells by regulating the transcription of tight junction molecules.
authors.affiliation: Department of Life Science, College of Natural Science, Daejin University, Pocheon 487-711, Korea.
authors.forename: Seung Taek
authors.initials: ST
authors.lastname: Nam
authors.affiliation: Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea.
authors.forename: Jung Hwan
authors.initials: JH
authors.lastname: Hwang
authors.affiliation: Department of Life Science, College of Natural Science, Daejin University, Pocheon 487-711, Korea.
authors.forename: Dae Hong
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Department of Life Science, College of Natural Science, Daejin University, Pocheon 487-711, Korea.
authors.forename: Mi Jung
authors.initials: MJ
authors.lastname: Park
authors.affiliation: Department of Life Science, College of Natural Science, Daejin University, Pocheon 487-711, Korea.
authors.forename: Ik Hwan
authors.initials: IH
authors.lastname: Lee
authors.affiliation: Department of Life Science, College of Natural Science, Daejin University, Pocheon 487-711, Korea.
authors.forename: Hyo Jung
authors.initials: HJ
authors.lastname: Nam
authors.affiliation: Department of Life Science, College of Natural Science, Daejin University, Pocheon 487-711, Korea.
authors.forename: Jin Ku
authors.initials: JK
authors.lastname: Kang
authors.affiliation: Department of Life Science, College of Natural Science, Daejin University, Pocheon 487-711, Korea.
authors.forename: Sung Kuk
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Suwon 441-707, Korea.
authors.forename: Jae Sam
authors.initials: JS
authors.lastname: Hwang
authors.affiliation: Department of Internal Medicine, Chungnam National University, Daejon 301-721, Korea.
authors.forename: Hyo Kyun
authors.initials: HK
authors.lastname: Chung
authors.affiliation: Department of Internal Medicine, Chungnam National University, Daejon 301-721, Korea.
authors.forename: Minho
authors.initials: M
authors.lastname: Shong
authors.affiliation: Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea.
authors.forename: Chul-Ho
authors.initials: CH
authors.lastname: Lee
authors.affiliation: Department of Internal Medicine, Chungnam National University, Daejon 301-721, Korea.
authors.forename: Ho
authors.initials: H
authors.lastname: Kim
mesh_terms: D000818:Animals; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D062445:Claudin-1 / Q000378:metabolism; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D015536:Down-Regulation; D004847:Epithelial Cells / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D006655:Histone Deacetylases / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D062793:Occludin / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D019108:Tight Junctions / Q000201:enzymology* / Q000378:metabolism
chemical_list: D062445:Claudin-1; D062793:Occludin; D017382:Reactive Oxygen Species; D016660:NAD(P)H Dehydrogenase (Quinone); D006655:Histone Deacetylases