title: [Application of PulseNet China Database in tracking and warning 4 cholerae outbreaks in Anhui province, in 2012].
abstract: OBJECTIVE
  To track the source of infection regarding 4 Cholerae outbreaks in Anhui province in 2012 through the application of PulseNet China Database (PNCD).
  
  
  METHODS
  Cholerae virulence genes were amplified by PCR and typed by pulse field gel electrophoresis(PFGE). Results from electrophoresis were cluster-analyzed by BioNumericsV4.0 software and compared with PNCD.
  
  
  RESULTS
  Virulence gene CT and TCP of the tested vibrio cholera showed both positive. Homology of the strains from four cholera outbreaks was more than 98%, based on the homologous and cluster analysis through enzyme digested PFGE electrophoresis. Those strains were highly homologous with the cholera epidemic strains identified in Hunan, Sichuan,Zhejiang, Shanghai and Hubei by PNCD, with the homology as 100% .
  
  
  CONCLUSIONS
  Four cholera outbreaks in Anhui province, 2012 were highly correlated with the outbreaks occurring in Hunan and Sichuan during the same time period, indicating that PNCD could effectively and quickly tracking down the source of infection on the cholera outbreaks and providing early warning of the situation.
authors.affiliation: Anhui Center for Disease Control and Prevention, Hefei 230061, China.
authors.forename: Shou-kui
authors.initials: SK
authors.lastname: Hu
authors.affiliation: College of Public Health, Sichuan University.
authors.forename: Tian-li
authors.initials: TL
authors.lastname: Zheng
authors.affiliation: Anhui Center for Disease Control and Prevention, Hefei 230061, China.
authors.forename: Wan-fu
authors.initials: WF
authors.lastname: Hu
authors.affiliation: Anhui Center for Disease Control and Prevention, Hefei 230061, China. Email: wjj@ahcdc.com.cn.
authors.forename: Jian-jun
authors.initials: JJ
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Infectious Disease Control and Prevention,Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: State Key Laboratory for Infectious Disease Control and Prevention,Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention.
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Cui
authors.affiliation: State Key Laboratory for Infectious Disease Control and Prevention,Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention.
authors.forename: Hai-jian
authors.initials: HJ
authors.lastname: Zhou
authors.affiliation: State Key Laboratory for Infectious Disease Control and Prevention, Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention.
authors.forename: Bo
authors.initials: B
authors.lastname: Pang
authors.affiliation: State Key Laboratory for Infectious Disease Control and Prevention,Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention.
authors.forename: Biao
authors.initials: B
authors.lastname: Kan
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods; D002681:China / Q000453:epidemiology; D002771:Cholera / Q000453:epidemiology* / Q000517:prevention & control*; D016000:Cluster Analysis; D004269:DNA, Bacterial; D016208:Databases, Factual; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction; D014734:Vibrio cholerae / Q000235:genetics
chemical_list: D004269:DNA, Bacterial