title: Identification of Bifidobacterium spp. using hsp60 PCR-RFLP analysis: an update.
abstract: A PCR-RFLP technique has been applied on 13 species of Bifidobacterium in order to update a previous study carried out by Baffoni et al. This method is based on the restriction endonuclease activity of HaeIII on the PCR-amplified hsp60 partial gene sequence, and allows a rapid and efficient identification of Bifidobacterium spp. strains at species and subspecies level.
authors.affiliation: Department of Agricultural Sciences, University of Bologna, Viale Fanin 42, 40127 Bologna, Italy.
authors.forename: Verena
authors.initials: V
authors.lastname: Stenico
authors.affiliation: Department of Agricultural Sciences, University of Bologna, Viale Fanin 42, 40127 Bologna, Italy. Electronic address: samanta.michelini2@unibo.it.
authors.forename: Samanta
authors.initials: S
authors.lastname: Michelini
authors.affiliation: Department of Agricultural Sciences, University of Bologna, Viale Fanin 42, 40127 Bologna, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Modesto
authors.affiliation: Department of Agricultural Sciences, University of Bologna, Viale Fanin 42, 40127 Bologna, Italy.
authors.forename: Loredana
authors.initials: L
authors.lastname: Baffoni
authors.affiliation: Department of Agricultural Sciences, University of Bologna, Viale Fanin 42, 40127 Bologna, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Mattarelli
authors.affiliation: Department of Agricultural Sciences, University of Bologna, Viale Fanin 42, 40127 Bologna, Italy.
authors.forename: Bruno
authors.initials: B
authors.lastname: Biavati
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D001431:Bacteriological Techniques / Q000379:methods*; D001644:Bifidobacterium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D018834:Chaperonin 60 / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases
keywords: Bifidobacterium; Identification; PCR-RFLP; hsp60