title: Cloning and bioinformatic analysis of full-length novel pepper (Capsicum annuum) genes TAF10 and TAF13.
abstract: We isolated two TATA-binding protein-associated factor (TAF) genes, TAF10 and TAF13, from pepper (Capsicum annuum). The complete coding sequences were amplified using reverse transcriptase-PCR on the basis of conserved sequence information of eggplant and several other plant species. Nucleotide sequence analysis of these two genes revealed that the pepper TAF10 gene encodes a protein of 103 amino acids that belongs to the TAF10 superfamily. The pepper TAF10 gene was highly expressed in the pericarp and placenta, moderately expressed in the stems, flowers, seeds and leaves, and weakly expressed in roots. The TAF13 gene was found to encode a protein of 130 amino acids that belongs to the TAF13 superfamily. The TAF13 gene was highly expressed in the stems, flowers and pericarp, moderately expressed in the leaves, placenta and seeds, and weakly expressed in roots.
authors.affiliation: Kunming University of Science and Technology, Kunming, China.
authors.forename: J F
authors.initials: JF
authors.lastname: Wen
authors.forename: J L
authors.initials: JL
authors.lastname: Huo
authors.forename: H X
authors.initials: HX
authors.lastname: Chen
authors.forename: C H
authors.initials: CH
authors.lastname: Ma
authors.forename: H
authors.initials: H
authors.lastname: Jiang
authors.forename: H S
authors.initials: HS
authors.lastname: Zhu
authors.forename: H
authors.initials: H
authors.lastname: Zhou
authors.forename: M H
authors.initials: MH
authors.lastname: Deng
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002212:Capsicum / Q000235:genetics*; D003001:Cloning, Molecular; D035264:Flowers / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics; D018517:Plant Roots / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D035463:Pol1 Transcription Initiation Complex Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000378:metabolism; D017422:Sequence Analysis, DNA; D035381:TATA-Binding Protein Associated Factors / Q000096:biosynthesis / Q000235:genetics*; D019473:Transcription Factors, TFII / Q000235:genetics
chemical_list: D010940:Plant Proteins; D035463:Pol1 Transcription Initiation Complex Proteins; D035381:TATA-Binding Protein Associated Factors; C065173:TFID transcription factor; D019473:Transcription Factors, TFII