title: Concomitant microRNA-31 downregulation and radixin upregulation predicts advanced tumor progression and unfavorable prognosis in patients with gliomas.
abstract: OBJECTIVE
  To clarify the clinical significance of microRNA-31 (miR-31) and radixin (RDX) in human glioma.
  
  
  METHODS
  Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to characterize the expression patterns of miR-31 and RDX mRNA in 108 glioma and 20 normal brain tissues. The associations of miR-31 and RDX mRNA expressions with clinicopathologic factors and prognosis of glioma patients were also statistically analyzed.
  
  
  RESULTS
  The expression levels of miR-31 in glioma tissues were significantly lower than those in normal brain tissues (P<0.001), while RDX mRNA was significantly overexpressed in glioma tissues compared with normal brain tissues (P<0.001). There was a negative correlation between miR-31 and RDX mRNA expression in glioma tissues (r=-0.69, P=0.01). Additionally, concomitant miR-31 downregulation and RDX upregulation (miR-31-low/RDX-high) was significantly associated with advanced pathological grade (P=0.001) and low Karnofsky performance score (P=0.01). Moreover, Kaplan-Meier survival and Cox regression analyses showed that the glioma patients with miR-31-low/RDX-high expression had poorest overall survival (P<0.001) and conjoined expression of miR-31-low/RDX-high was an independent prognostic indicator of glioma (P=0.01). Furthermore, subgroup analyses showed that miR-31-low/RDX-high expression was significantly associated with poor overall survival in glioma patients with high pathological grades (for grade III-IV: P<0.001).
  
  
  CONCLUSIONS
  Our findings have implications concerning the importance of concomitant miR-31 downregulation and RDX upregulation in tumor progression and poor prognosis of patients with gliomas. A combined detection of miR-31/RDX expression may benefit us in predicting clinical outcomes of glioma patients with high pathological grades.
authors.affiliation: Department of Neurosurgery, Second Hospital of Hebei Medical University, No. 215, Hepingxi Road, Shijiazhuang City, Hebei Province 050000 China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Second Hospital of Hebei Medical University, No. 215, Hepingxi Road, Shijiazhuang City, Hebei Province 050000 China.
authors.forename: Baohua
authors.initials: B
authors.lastname: Jiao
authors.affiliation: Department of Neurosurgery, Second Hospital of Hebei Medical University, No. 215, Hepingxi Road, Shijiazhuang City, Hebei Province 050000 China.
authors.forename: Shaomei
authors.initials: S
authors.lastname: Geng
authors.affiliation: Department of Neurosurgery, Second Hospital of Hebei Medical University, No. 215, Hepingxi Road, Shijiazhuang City, Hebei Province 050000 China.
authors.forename: Jian
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Neurosurgery, Second Hospital of Hebei Medical University, No. 215, Hepingxi Road, Shijiazhuang City, Hebei Province 050000 China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Department of Neurosurgery, Second Hospital of Hebei Medical University, No. 215, Hepingxi Road, Shijiazhuang City, Hebei Province 050000 China. Electronic address: lskhbey@163.com.
authors.forename: Shengkui
authors.initials: S
authors.lastname: Lu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000235:genetics* / Q000401:mortality / Q000532:radiotherapy; D016009:Chi-Square Distribution; D003598:Cytoskeletal Proteins / Q000235:genetics* / Q000378:metabolism; D018450:Disease Progression; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D005910:Glioma / Q000235:genetics* / Q000401:mortality / Q000532:radiotherapy; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D011237:Predictive Value of Tests; D012333:RNA, Messenger / Q000378:metabolism; D016019:Survival Analysis; D055815:Young Adult
chemical_list: D003598:Cytoskeletal Proteins; C534612:MIRN31 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; D012333:RNA, Messenger; C071776:radixin
keywords: Clinicopathologic characteristics; Expression; Glioma; MicroRNA-31; Prognosis; Radixin