title: [Micro RNA-155 expression in hemodialysis PBMC].
abstract: BACKGROUND
  MicroRNAs (miR) are fragments of non-coding RNA acting at post-transcriptional level, which modulate gene expression, and play a key role in several pathophysiological pathways. The aim of this study is to analyze the expression of miR-155 in basal Peripheral Blood Mononuclear Cells (PBMC) of chronic hemodialysis (HD) patients, before and after standard 4 -hour sessions, and after PHA stimulation compared with PBMC from healthy subjects.
  
  
  METHODS
  miR-155 was isolated from chronic HD patients' PBMC and from PBMC derived from healthy subjects. Expression levels were quantified with Real-Time PCR; PCR reactions were performed using a specific thermocycler and cycle threshold levels were calculated using dedicated software. Blood samples were taken from HD patients after the long inter-dialytic interval. Data were expressed as MSE and statistical differences were calculated with t-test.
  
  
  RESULTS
  Relative quantity (RQ) of pre-dialysis MiR-155 was 3.770.62 times higher than the control group (P=0,003). There was no significant difference before and after hemodialysis sessions. Pre-dialysis mir-155 RQ in PHA PBMC was 1.790.59 times higher than non stimulated PBMC (P=0.019).
  
  
  CONCLUSIONS
  these preliminary data show a significant miR-155 up-regulation of HD PBMC when compared to PBMC from healthy individuals. An additional up-regulation was observed in HD PHA PBMC. Similar miR-155 expression was found in HD PBMC comparing pre and post-hemodialysis sessions.
authors.forename: Paolo
authors.initials: P
authors.lastname: Albrizio
authors.forename: M
authors.initials: M
authors.lastname: Gnecchi
authors.forename: E
authors.initials: E
authors.lastname: Cervio
authors.forename: F
authors.initials: F
authors.lastname: Mangione
authors.forename: F
authors.initials: F
authors.lastname: Fiorini
authors.forename: Rt
authors.initials: R
authors.lastname: Rampino
authors.forename: C
authors.initials: C
authors.lastname: Libetta
authors.forename: A
authors.initials: A
authors.lastname: Dal Canton
mesh_terms: D000368:Aged; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D008875:Middle Aged; D006435:Renal Dialysis
chemical_list: C524942:MIRN155 microRNA, human; D035683:MicroRNAs