title: NGF induces the expression of group IIA secretory phospholipase A2 in PC12 cells: the newly synthesized enzyme is addressed to growing neurites.
abstract: We proposed that group IIA secretory phospholipase A(2) (GIIA) participates in neuritogenesis based on our observations that the enzyme migrates to growth cones and neurite tips when PC12 cells are induced to differentiate by nerve growth factor (NGF) (Ferrini et al., Neurochem Res 35:2168-2174, 2010). The involvement of other secretory PLA(2) isoforms in neuronal development has been suggested by others but through different mechanisms. In the present study, we compared the subcellular distribution of GIIA and group X sPLA(2) (GX) after stimulation of PC12 cells with NGF. We found that GIIA, but not GX, localized at the neuritic tips after treatment with NGF, as demonstrated by immunofluorescence analysis. We also found that NGF stimulated the expression and the activity of GIIA. In addition, NGF induced the expressed myc-tagged GIIA protein to migrate to neurite tips in its active form. We propose that GIIA expression, activity, and subcellular localization is regulated by NGF and that the enzyme may participate in neuritogenesis through intracellular mechanisms, most likely by facilitating the remodelling of glycerophospholipid molecular species by deacylation-reacylation reactions necessary for the incorporation of polyunsaturated fatty acids.
authors.affiliation: Department of Internal Medicine, Neurochemistry Laboratory, University of Perugia, Via del Giochetto, 06126, Perugia, Italy.
authors.forename: Vincenza
authors.initials: V
authors.lastname: Nardicchi
authors.forename: Monica
authors.initials: M
authors.lastname: Ferrini
authors.forename: Francesca
authors.initials: F
authors.lastname: Pilolli
authors.forename: Emanuela Biagioni
authors.initials: EB
authors.lastname: Angeli
authors.forename: Emanuele
authors.initials: E
authors.lastname: Persichetti
authors.forename: Tommaso
authors.initials: T
authors.lastname: Beccari
authors.forename: Roberta
authors.initials: R
authors.lastname: Mannucci
authors.forename: Cataldo
authors.initials: C
authors.lastname: Arcuri
authors.forename: Rosario
authors.initials: R
authors.lastname: Donato
authors.forename: Robert V
authors.initials: RV
authors.lastname: Dorman
authors.forename: Gianfrancesco
authors.initials: G
authors.lastname: Goracci
mesh_terms: D000818:Animals; D054501:Group II Phospholipases A2 / Q000235:genetics / Q000378:metabolism*; D020932:Nerve Growth Factor / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects / Q000201:enzymology*; D055495:Neurogenesis / Q000187:drug effects*; D016716:PC12 Cells; D051381:Rats
chemical_list: D020932:Nerve Growth Factor; D054501:Group II Phospholipases A2