title: Evaluation of the wound-healing activity and anti-inflammatory activity of aqueous extracts from Acorus calamus L.
abstract: In folklore medicine, Acorus calamus has been used as a wound-healing agent for thousands of years; however, there have been few scientific reports on this activity so far. Now, we explored deeply the wound-healing effect of aqueous extracts from the fresh roots and rhizomes of A. calamus in vivo, as well as anti-inflammatory activity in vitro, so as to provide scientific evidence for the traditional application. The wound-healing effect was determined by the image analysis techniques and the histological analysis in the excisional wounding test, and the anti-inflammatory activity was evaluated by the real-time RT-PCR techniques in the lipopolysaccharide-induced RAW 264.7 cells test. Aqueous extracts, administered topically at the dose range from twice to thrice in a day, could enhance significantly the rate of skin wound-healing. Moreover, the extracts could effectively inhibit the mRNA expressions of inflammatory mediators induced by lipopolysaccharide in RAW 264.7 cells. These results showed significantly the wound-healing activity of aqueous extracts in the animal model of excise wound healing, and anti-inflammatory activity in vitro.
authors.affiliation: Department of Pharmacy, Shen-yang North Hospital, 83# Wenhua Road, Shen-yang, PR China.
authors.forename: Guo-bing
authors.initials: GB
authors.lastname: Shi
authors.affiliation: Department of Clinical Laboratory, Shen-yang North Hospital, 83# Wenhua Road, Shen-yang, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Shen-yang North Hospital, 83# Wenhua Road, Shen-yang, PR China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Pharmacy, Shen-yang North Hospital, 83# Wenhua Road, Shen-yang, PR China.
authors.forename: Tong-chao
authors.initials: TC
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Shen-yang North Hospital, 83# Wenhua Road, Shen-yang, PR China.
authors.forename: Chang-li
authors.initials: CL
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Shen-yang North Hospital, 83# Wenhua Road, Shen-yang, PR China.
authors.forename: Xue-hui
authors.initials: XH
authors.lastname: Sun
authors.affiliation: Department of Pharmacy, Shen-yang North Hospital, 83# Wenhua Road, Shen-yang, PR China.
authors.forename: Wen-tao
authors.initials: WT
authors.lastname: Zong
authors.affiliation: Department of Pharmacy, Shen-yang North Hospital, 83# Wenhua Road, Shen-yang, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yan
authors.affiliation: Department of Pharmacy, Shen-yang North Hospital, 83# Wenhua Road, Shen-yang, PR China.
authors.forename: Qing-chun
authors.initials: QC
authors.lastname: Zhao
authors.affiliation: Department of Pharmacy, Shen-yang North Hospital, 83# Wenhua Road, Shen-yang, PR China.
authors.forename: Yu-feng
authors.initials: YF
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, Shen-yang North Hospital, 83# Wenhua Road, Shen-yang, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D029023:Acorus; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D005260:Female; D018836:Inflammation Mediators / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000893:Anti-Inflammatory Agents; D018836:Inflammation Mediators; D010936:Plant Extracts