title: Sequential protein NMR assignments in the liquid state via sequential data acquisition.
abstract: Two different NMR pulse schemes involving sequential (1)H data acquisition are presented for achieving protein backbone sequential resonance assignments: (i) acquisition of 3D {HCCNH and HNCACONH} and (ii) collection of 3D {HNCOCANH and HNCACONH} chemical shift correlation spectra using uniformly (13)C,(15)N labelled proteins. The sequential acquisition of these spectra reduces the overall experimental time by a factor of ≈2 as compared to individual acquisitions. The suitability of this approach is experimentally demonstrated for the C-terminal winged helix (WH) domain of the minichromosome maintenance (MCM) complex of Sulfolobus solfataricus.
authors.affiliation: Research Group Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research, Fritz Lipmann Institute, Beutenbergstr. 11, D-07745 Jena, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Wiedemann
authors.affiliation: Research Group Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research, Fritz Lipmann Institute, Beutenbergstr. 11, D-07745 Jena, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Bellstedt
authors.affiliation: Research Group Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research, Fritz Lipmann Institute, Beutenbergstr. 11, D-07745 Jena, Germany.
authors.forename: Anika
authors.initials: A
authors.lastname: Kirschstein
authors.affiliation: Research Group Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research, Fritz Lipmann Institute, Beutenbergstr. 11, D-07745 Jena, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Häfner
authors.affiliation: Department of Physics, Faculty of Science, Ubon Ratchathani University, 34190 Ubon Ratchathani, Thailand.
authors.forename: Christian
authors.initials: C
authors.lastname: Herbst
authors.affiliation: Research Group Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research, Fritz Lipmann Institute, Beutenbergstr. 11, D-07745 Jena, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Görlach
authors.affiliation: Research Group Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research, Fritz Lipmann Institute, Beutenbergstr. 11, D-07745 Jena, Germany. Electronic address: raman@fli-leibniz.de.
authors.forename: Ramadurai
authors.initials: R
authors.lastname: Ramachandran
mesh_terms: D002247:Carbon Isotopes; D002876:Chromosomes, Bacterial / Q000737:chemistry; D004574:Electromagnetic Fields; D007202:Indicators and Reagents; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D048229:Sulfolobus solfataricus / Q000737:chemistry
chemical_list: D002247:Carbon Isotopes; D007202:Indicators and Reagents; D009587:Nitrogen Isotopes; D011506:Proteins
keywords: Minichromosome maintenance (MCM); NMR; Protein resonance assignment; Sequential data acquisition; Winged helix domain (WH)