title: The G1057D polymorphism of insulin receptor substrate-2 associated with gestational diabetes mellitus.
abstract: OBJECTIVE
  The Gly1057D polymorphism in the insulin receptor substrate-2 (IRS-2) gene has been reported to be associated with insulin resistance, obesity and type 2 diabetes; little is known about its possible association with gestational diabetes mellitus (GDM). To investigate this association we determined the distribution of its genotypes and frequency of alleles in GDM patients.
  
  
  METHODS
  The study population consisted of 94 subjects; among them were 44 patients with GDM and 50 healthy controls without diabetes. Genomic DNA was extracted from the leukocyte by high pure polymerase chain reaction (PCR) template preparation kit. Genetic polymorphism of IRS-2 G1057D was detected by using PCR-based restriction fragment-length polymorphism (RFLP).
  
  
  RESULTS
  For IRS-2 G1057D polymorphism, there was no significant difference in genotype distribution between GDM patients and controls. The risk for GDM was 2.97 times higher (95% CI: 0.89-9.93, p = 0.076) in the individuals with the IRS-2 DD genotype compared to the GG genotype. Also individuals with the IRS-2 D allele had a significantly higher risk of GDM compared with individuals with the IRS-2 G allele, with a relative risk of 1.86 (95% CI: 1.02-3.37, p = 0.042) for cases compared with population controls.
  
  
  CONCLUSIONS
  These results suggest that IRS-2 1057D allele may be associated with GDM.
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Trakya University , Edirne , Turkey .
authors.forename: Lokman
authors.initials: L
authors.lastname: Ayaz
authors.forename: Sevim
authors.initials: S
authors.lastname: Karakaş Çelik
authors.forename: Filiz
authors.initials: F
authors.lastname: Cayan
mesh_terms: D000328:Adult; D000483:Alleles; D016640:Diabetes, Gestational / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy
chemical_list: C526297:IRS2 protein, human; D055504:Insulin Receptor Substrate Proteins