title: Preparation of MIP-based QCM nanosensor for detection of caffeic acid.
abstract: In the present work, a new caffeic acid imprinted quartz crystal microbalance (QCM) nanosensor has been designed for selective assignation of caffeic acid in plant materials. Methacrylamidoantipyrine-iron(III) [MAAP-Fe(III)] as metal-chelating monomer has been used to prepare selective molecular imprinted polymer (MIP). MIP film for detection of caffeic acid has been developed on QCM electrode and selectivity experiments and analytical performance of caffeic acid imprinted QCM nanosensor has been studied. The caffeic acid imprinted QCM nanosensor has been characterized by AFM. After the characterization studies, imprinted and non-imprinted nanosensors was connected to QCM system for studies of connection of the target molecule, selectivity and the detection of amount of target molecule in real samples. The detection limit was found to be 7.8 nM. The value of Langmuir constant (b) (4.06 × 10(6)) that was acquired using Langmuir graph demonstrated that the affinity of binding sites was strong. Also, selectivity of prepared caffeic acid imprinted nanosensor was found as being high compared to chlorogenic acid. Finally, the caffeic acid levels in plant materials was determined by the prepared QCM nanosensor.
authors.affiliation: Department of Energy Systems Engineering, Faculty of Engineering, Karamanoğlu Mehmetbey University, Karaman 70200, Turkey. Electronic address: aysari@yahoo.com.
authors.forename: Aytaç
authors.initials: A
authors.lastname: Gültekin
authors.affiliation: Department of Energy Systems Engineering, Faculty of Engineering, Karamanoğlu Mehmetbey University, Karaman 70200, Turkey.
authors.forename: Gamze
authors.initials: G
authors.lastname: Karanfil
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Selçuk University, Konya, Turkey.
authors.forename: Mahmut
authors.initials: M
authors.lastname: Kuş
authors.affiliation: Department of Material Science and Engineering, Faculty of Engineering, Karamanoğlu Mehmetbey University, Karaman 70200, Turkey.
authors.forename: Savaş
authors.initials: S
authors.lastname: Sönmezoğlu
authors.affiliation: Department of Chemistry, Faculty of Science, Anadolu University, Eskisehir, Turkey.
authors.forename: Rıdvan
authors.initials: R
authors.lastname: Say
mesh_terms: D002109:Caffeic Acids / Q000032:analysis*; D057230:Limit of Detection; D018625:Microscopy, Atomic Force; D054802:Molecular Imprinting; D036103:Nanotechnology; D011108:Polymers / Q000737:chemistry*; D058525:Quartz Crystal Microbalance Techniques
chemical_list: D002109:Caffeic Acids; D011108:Polymers; C040048:caffeic acid
keywords: Caffeic acid; MIP; Nanosensor; QCM