title: [HIV-1 infection up-regulating expression of interferon-stimulated gene 15 in cell lines].
abstract: To investigate whether HIV-1 infection affects expression of interferon-stimulated gene 15 (ISG15) and determine the antiviral effect of ISG15 in vitro, ISG15 expression at transcription and protein level and supernatant p24 of HIV-1 was detected in various HIV-1 infected or transfected cell lines, respec tively. HIV-1 molecular clone pNL4-3 was used to transfect 293T, TZM-bl and HeLa cells while HIV-1 pseudo-typed virus was used to infect Jurkat, MT-1 and THP-1 cells. After twenty-four hours post infection or transfection, cells were harvested for extraction of total RNAs and subsequently used in real time PCR for quantification of ISG15 transcriptional expression. After forty-eight hours post infection or transfection, cells were harvested for extraction of total proteins to detect ISG15 protein expression. A significant up-regulation of ISG15 at transcription level was observed in HIV-1 infected or transfected cell lines, particulaly in THP-1 and TZM-bl cells. Up-regulation of ISG15 protein was observed only in TZM-bl cell. Cotransfection of ISG15 and HIV-1 indicated that ISG15 inhibited production of HIV-1 progeny virus in a dose and time depend manner in 293T cell but not TZM-bl cell. These results revealed upregulating ISG15 expression in transcriptional level and potential antagonistic mechanism against ISG15 by HIV-1 infection, simultanelusly.
authors.affiliation: Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China. dianan7@163.com
authors.forename: Huan-mei
authors.initials: HM
authors.lastname: Wu
authors.affiliation: Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Sun
authors.affiliation: Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China.
authors.forename: Zhe-feng
authors.initials: ZF
authors.lastname: Meng
authors.affiliation: Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China.
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China.
authors.forename: Jian-qing
authors.initials: JQ
authors.lastname: Xu
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D015658:HIV Infections / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D007372:Interferons / Q000378:metabolism; D008969:Molecular Sequence Data; D014452:Ubiquitins / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D016207:Cytokines; D014452:Ubiquitins; C074034:ISG15 protein, human; D007372:Interferons