title: Oleanolic acid suppresses ovalbumin-induced airway inflammation and Th2-mediated allergic asthma by modulating the transcription factors T-bet, GATA-3, RORγt and Foxp3 in asthmatic mice.
abstract: The natural product oleanolic acid is commonly found in a variety of medicinal plants. It is a triterpenoid compound known for its anti-inflammatory effects as well as various other pharmacological properties. The aim of the current study was to use a mouse model of allergic asthma to investigate whether oleanolic acid has anti-asthmatic effects, and if so, to determine the mechanism of these effects. Oleanolic acid suppressed eosinophil infiltration, allergic airway inflammation, and Penh, which occurred by suppressing the production of IL-5, IL-13, IL-17, and ovalbumin-specific IgE through the upregulation of T-bet and Foxp3, and the downregulation of GATA-3 and RORγt. The therapeutic effect of oleanolic acid was due to suppression of Th2 cytokines (IL-5 and IL-13), B cell-dependent production of OVA-specific IgE, and Gr-1 expression through the T-bet, GATA-3, retinoic acid-related orphan receptor γ t (RORγ t) and forkhead box p3 (Foxp3) transcription pathways. It is therefore reasonable to suggest that the anti-inflammatory and anti-asthmatic effects of oleanolic acid may be exerted through inhibition of the GATA-3 and RORγt pathways.
authors.affiliation: Institute of Traditional Medicine & Bioscience, Daejeon University, Daejeon 300-716, Republic of Korea.
authors.forename: Seung-Hyung
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Herbology, College of Oriental Medicine, Sangji University, Wonju 220-702, Republic of Korea.
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Hong
authors.affiliation: Department of Herbology, College of Oriental Medicine, Sangji University, Wonju 220-702, Republic of Korea. Electronic address: lyc072@sangji.ac.kr.
authors.forename: Young-Cheol
authors.initials: YC
authors.lastname: Lee
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001249:Asthma / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D016535:Bronchial Hyperreactivity / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D045744:Cell Line, Tumor; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D004804:Eosinophils / Q000276:immunology; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000276:immunology; D050990:GATA3 Transcription Factor / Q000235:genetics / Q000276:immunology; D008168:Lung / Q000276:immunology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000276:immunology; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3 / Q000276:immunology; D009828:Oleanolic Acid / Q000494:pharmacology* / Q000627:therapeutic use; D010047:Ovalbumin / Q000276:immunology; D011014:Pneumonia / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D020825:T-Box Domain Proteins / Q000235:genetics / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D000098815:T-bet Transcription Factor
chemical_list: D000485:Allergens; D018927:Anti-Asthmatic Agents; D016207:Cytokines; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D009828:Oleanolic Acid; D010047:Ovalbumin
keywords: Asthma; GATA-3; IL-17; Oleanolic acid; RORγt; Th2