title: Facile access to cytocompatible multicompartment micelles with adjustable Janus-cores from A-block-B-graft-C terpolymers prepared by combination of ROP and ATRP.
abstract: The architecture of hydrophobic segments can determine the specific morphology of multicompartment micelles (MCMs) that are generated from aqueous assembly of amphiphilic terpolymers. In this study, we aimed to design and generate poly(ɛ-caprolactone)-based multicompartment micelles with adjustable Janus-cores. Well-defined terpolymers with a novel A-block-B-graft-C architecture composed of biologically compatible polymers, methoxy poly(ethylene glycol) (PEG), poly(ɛ-caprolactone) (PCL) and poly(2-(perfluorobutyl)ethyl methacrylate) (PPFEMA), were prepared by the stepwise use of ring-opening polymerization and atom transfer radical polymerization. Characterization of the obtained terpolymers was carried out by (1)H NMR and gel permeation chromatography. Results from differential scanning calorimetry and X-ray diffraction studies indicated that within the terpolymer structure, the PCL segments are in the crystalline state, while fluorocarbon segments belong to the amorphous domains. Due to the thermodynamic incompatibility of PCL and PPFEMA, MCMs could be obtained upon aqueous self-assembly of the terpolymer. The well-segregated Janus-cores with adjustable compartment balance were revealed by transmission electron microscopy. In vitro cell viability assays further demonstrated an excellent cytocompatibility of the MCMs both in mouse embryonic fibroblasts (3T3) and human acute monocytic leukemia (THP-1) cells.
authors.affiliation: Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering, Chinese Academy of Medical Science, Tianjin 300192, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering, Chinese Academy of Medical Science, Tianjin 300192, China.
authors.forename: Ju
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering, Chinese Academy of Medical Science, Tianjin 300192, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Pingsheng
authors.initials: P
authors.lastname: Huang
authors.affiliation: Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering, Chinese Academy of Medical Science, Tianjin 300192, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Gao
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Shangcong
authors.initials: S
authors.lastname: Han
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. Electronic address: ajdong@tju.edu.cn.
authors.forename: Anjie
authors.initials: A
authors.lastname: Dong
authors.affiliation: Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering, Chinese Academy of Medical Science, Tianjin 300192, China. Electronic address: kongdeling@hotmail.com.
authors.forename: Deling
authors.initials: D
authors.lastname: Kong
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002152:Calorimetry, Differential Scanning; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D008823:Micelles; D010316:Particle Size; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D058105:Polymerization / Q000187:drug effects; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D012542:Scattering, Radiation; D014961:X-Ray Diffraction
chemical_list: D008689:Methacrylates; D008823:Micelles; D011091:Polyesters; D011108:Polymers; C016240:polycaprolactone; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol
keywords: Amphiphilic terpolymers; Graft architecture; Janus-core; Microphase separation; Multicompartment micelles