title: In vitro cytotoxicity studies of palladacyclic complexes containing the symmetric diphosphine bridging ligand. Studies of their interactions with DNA and BSA.
abstract: The reactions between [Pd2{(C,N)-C6H4CH2NH(Et)}2(μ-X)2] (X = Cl or Br) and 1,2-bis(diphenylphosphino)ethane (dppe) in the 1:1 molar ratio resulted in the dppe-bridged Pd(II) complexes, [Pd2{(C,N)-C6H4CH2NH(Et)}2(μ-dppe)(Cl)2] (1) and [Pd2{(C,N)-C6H4CH2NH(Et)}2(μ-dppe)(Br)2] (2), respectively, which were characterized by elemental analyses, infrared (IR), (1)H- and (31)P{(1)H} NMR spectroscopy. The molecular structure of 1 was determined by single-crystal X-ray diffraction. In vitro cytotoxicity of 1, 2, dppe, PhCH2NH(Et) and cisplatin were carried out against four human tumor cell lines. The interactions of complexes towards DNA and protein are investigated. The results suggested that both complexes could interact with FS-DNA through the intercalation mode. Moreover, the reactivity towards BSA revealed that the microenvironment and the secondary structure of BSA were changed in the presence of Pd(II) complexes.
authors.affiliation: Department of Chemistry, Isfahan University of Technology, Isfahan 84156/83111, Iran. Electronic address: karami@cc.iut.ac.ir.
authors.forename: Kazem
authors.initials: K
authors.lastname: Karami
authors.affiliation: Department of Chemistry, Isfahan University of Technology, Isfahan 84156/83111, Iran. Electronic address: m.hosseinikharat@ch.iut.ac.ir.
authors.forename: Mahboubeh
authors.initials: M
authors.lastname: Hosseini-Kharat
authors.affiliation: Department of Pharmaceutical Chemistry, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Hojjat
authors.initials: H
authors.lastname: Sadeghi-Aliabadi
authors.affiliation: Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Lipkowski
authors.affiliation: Department of Molecular Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Mina
authors.initials: M
authors.lastname: Mirian
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001665:Binding Sites; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry*; D006801:Humans; D007364:Intercalating Agents; D008024:Ligands; D008958:Models, Molecular; D015394:Molecular Structure; D010165:Palladium / Q000737:chemistry*; D010720:Phosphines / Q000737:chemistry*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D014961:X-Ray Diffraction
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D007364:Intercalating Agents; D008024:Ligands; D010720:Phosphines; D012710:Serum Albumin, Bovine; D010165:Palladium; D004247:DNA
keywords: BSA binding; Crystal structure; Cytotoxicity; FS-DNA binding; Palladacyclic complex