title: [Determination of eight ginsenosides in renshenshouwu capsules by HPLC-DAD].
abstract: OBJECTIVE
  To establish an HPLC-DAD method for simultaneous determination of eight ginsenosides in Renshenshouwu capsules.
  
  
  METHODS
  Ultimate C18 column (4.6 mm x 250 mm, 5 microm) was adopted for gradient elution, with acetonitrile and water as the mobile phase. The flow rate was 1 mL x min(-1), the column temperature was set at 30 degrees C, and the detection wavelength was 203 nm.
  
  
  RESULTS
  A good linearity was observed in the range of 0.242-12.1, 0.222-11.1, 0.251-25.1, 0. 245-24.5, 0.232-23.2, 0.232-23.2, 0.264-26.4, 0.244-24.4 microg for ginsenoside Rg1, Re, Rb1, Rc, Rb2, Rb3, Rd and 20(S)-ginsenoside Rg3, respectively, with the average recoveries of 102.7%, 103.2%, 101.6%, 101.2%, 102.0%, 100.7%, 101.9%, 102.0%, respectively.
  
  
  CONCLUSIONS
  The method was so simple, accurate and effective that it could be used for quality control of the above eight components in Renshenshouwu capsules.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. jidoudou@163.com
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Ji
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Feng
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Wang
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Zhu
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Qi-Wei
authors.initials: QW
authors.lastname: Zhang
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Liang-Mian
authors.initials: LM
authors.lastname: Chen
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Li
mesh_terms: D002214:Capsules / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D036145:Ginsenosides / Q000032:analysis*
chemical_list: D002214:Capsules; D004365:Drugs, Chinese Herbal; D036145:Ginsenosides