title: miR-126 functions as a tumor suppressor in osteosarcoma by targeting Sox2.
abstract: Osteosarcoma (OS) is the most common malignant bone tumor in children and young adults, the early symptoms and signs of which are non-specific. The discovery of microRNAs (miRNAs) provides a new avenue for the early diagnosis and treatment of OS. miR-126 has been reported to be highly expressed in vascularized tissues, and is recently widely studied in cancers. Herein, we explored the expression and significance of miR-126 in OS. Using TaqMan RT-PCR analysis, we analyzed the expression of miR-126 in 32 paired OS tumor tissues and 4 OS cell lines and found that miR-126 was consistently under-expressed in OS tissues and cell lines compared with normal bone tissues and normal osteoblast cells (NHOst), respectively. As miR-126 is significantly decreased in OS tissues and cell lines, we sought to compensate for its loss through exogenous transfection into MG-63 cells with a miR-126 mimic. Ectopic expression of miR-126 inhibited cell proliferation, migration and invasion, and induced apoptosis of MG-63 cells. Moreover, bioinformatic prediction suggested that the sex-determining region Y-box 2 (Sox2) is a target gene of miR-126. Using mRNA and protein expression analysis, luciferase assays and rescue assays, we demonstrate that restored expression of Sox2 dampened miR-126-mediated suppression of tumor progression, which suggests the important role of miR-126/Sox2 interaction in tumor progression. Taken together, our data indicate that miR-126 functions as a tumor suppressor in OS, which exerts its activity by suppressing the expression of Sox2.
authors.affiliation: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China. yangchenglindoctor@gmail.com.
authors.forename: Chenglin
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China. chunying_hou@126.com.
authors.forename: Chunying
authors.initials: C
authors.lastname: Hou
authors.affiliation: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China. zhanghepengdoctor@gmail.com.
authors.forename: Hepeng
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China. wdw19761224@gmail.com.
authors.forename: Dewei
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China. mayandoctor@gmail.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China. zhangyunqidoctor@gmail.com.
authors.forename: Yunqi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China. xxydoctor@gmail.com.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China. bizhenggang@163.com.
authors.forename: Zhenggang
authors.initials: Z
authors.lastname: Bi
authors.affiliation: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China. shuogeng.hmu@gmail.com.
authors.forename: Shuo
authors.initials: S
authors.lastname: Geng
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis; D001483:Base Sequence; D001859:Bone Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D055748:SOXB1 Transcription Factors / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D020413:3' Untranslated Regions; C527111:MIRN126 microRNA, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D055748:SOXB1 Transcription Factors