title: Low-dose radiation induces renal SOD1 expression and activity in type 1 diabetic mice.
abstract: OBJECTIVE
  Oxidative stress plays a critical role in the pathogenesis of diabetic nephropathy (DN). As an antioxidant, superoxide dismutase (SOD)-1 deficiency exacerbates but SOD1 supplementation prevents diabetes-induced renal damage. Previously, we have demonstrated that repetitive exposure to low-dose radiation (LDR) at 25 mGy significantly prevents DN. Whether this prevention is related to SOD1 expression and activity remains unknown. The aim of the present study was to explore the effects of different methods of LDR treatment on SOD1 expression and activity in the kidneys of diabetic mice.
  
  
  METHODS
  C57BL/6J mice were induced with type 1 diabetes using streptozotocin (STZ). Diabetic mice were irradiated with whole-body X-rays at either a single dose of 25 mGy or 75 mGy, or three doses of 25 mGy and then sacrificed at different times. Body weight, blood glucose level, and renal SOD1 expression and activity were measured.
  
  
  RESULTS
  LDR had no impact on the body weights or blood glucose levels of the mice in either the normal or diabetic groups. A single exposure of LDR at 25 mGy did not preserve renal SOD1 expression and activity in diabetic mice, but a single exposure of LDR at 75 mGy or three exposures of LDR at 25 mGy could preserve them.
  
  
  CONCLUSIONS
  The stimulation of renal SOD1 expression and activity by a single or cumulative LDR of 75 mGy may be one of the preventive mechanisms of DN observed in the previous study.
authors.affiliation: Ruian Center of the Chinese-American Research Institute for Diabetic Complications, the Third Affiliated Hospital of the Wenzhou Medical College , Wenzhou.
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.forename: Xiao
authors.initials: X
authors.lastname: Xing
authors.forename: Fangfang
authors.initials: F
authors.lastname: Zhang
authors.forename: Minglong
authors.initials: M
authors.lastname: Shao
authors.forename: Shuzi
authors.initials: S
authors.lastname: Jin
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Guanjun
authors.initials: G
authors.lastname: Wang
authors.forename: Jiuwei
authors.initials: J
authors.lastname: Cui
authors.forename: Lu
authors.initials: L
authors.lastname: Cai
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Xuemian
authors.initials: X
authors.lastname: Lu
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000528:radiation effects; D001835:Body Weight / Q000528:radiation effects; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic; D007668:Kidney / Q000201:enzymology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism*; D000072105:Superoxide Dismutase-1; D014965:X-Rays
chemical_list: D001786:Blood Glucose; C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1