title: Combined use of platelet rich plasma and vitamin C positively affects differentiation in vitro to mesodermal lineage of adult adipose equine mesenchymal stem cells.
abstract: Repair of injured soft and hard tissues in horses can benefit greatly from the use of regenerative therapies with mesenchymal stem cells (MSC). Vitamin-C and platelet-rich-plasma had been used for in vitro differentiation of MSC. This study was aimed to evaluate the effect of vitamin-C, platelet-rich-plasma and their combination on the in vitro differentiation of adipose horse MSC. We isolated MSC from horse fat and differentiated them in vitro into osteogenic and chondrogenic lineages, as demonstrated by specific staining and RT-qPCR of selected genes. Combining vitamin-C and plasma-rich-platelet positively affected the ability of MSC to differentiate in vitro into mesodermal lineages during 14 days of culture; this effect was not as marked when differentiation was attempted for 21 days. This provides valuable information on the effect of combined use of these molecules in regenerative therapies and their potential application along stem cells for lesions of musculoskeletal tissue in sport horses.
authors.affiliation: Department of Animal Science, Facultad de Ciencias Veterinarias, Universidad de Concepción, Avenida Vicente Méndez 595, Chillán, Chile. Electronic address: fidcastro@udec.cl.
authors.forename: F O
authors.initials: FO
authors.lastname: Castro
authors.affiliation: Department of Animal Science, Facultad de Ciencias Veterinarias, Universidad de Concepción, Avenida Vicente Méndez 595, Chillán, Chile.
authors.forename: A
authors.initials: A
authors.lastname: Torres
authors.affiliation: Department of Animal Science, Facultad de Ciencias Veterinarias, Universidad de Concepción, Avenida Vicente Méndez 595, Chillán, Chile.
authors.forename: J
authors.initials: J
authors.lastname: Cabezas
authors.affiliation: Department of Animal Science, Facultad de Ciencias Veterinarias, Universidad de Concepción, Avenida Vicente Méndez 595, Chillán, Chile.
authors.forename: Ll
authors.initials: L
authors.lastname: Rodríguez-Alvarez
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D005260:Female; D006651:Histocytochemistry / Q000662:veterinary; D006736:Horses / Q000378:metabolism*; D018960:Hyaluronan Receptors / Q000235:genetics / Q000378:metabolism; D019012:Integrin beta1 / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis / Q000502:physiology; D015676:Osteonectin / Q000235:genetics / Q000378:metabolism; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D055758:SOX9 Transcription Factor / Q000235:genetics
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D018960:Hyaluronan Receptors; D019012:Integrin beta1; D015676:Osteonectin; D055758:SOX9 Transcription Factor; D012313:RNA; D001205:Ascorbic Acid
keywords: Chondrogenic; Mesenchymal stem cells; Osteogenic; PRP; Vitamin C