title: Carbon source dependent promoters in yeasts.
abstract: Budding yeasts are important expression hosts for the production of recombinant proteins.The choice of the right promoter is a crucial point for efficient gene expression, as most regulations take place at the transcriptional level. A wide and constantly increasing range of inducible, derepressed and constitutive promoters have been applied for gene expression in yeasts in the past; their different behaviours were a reflection of the different needs of individual processes.Within this review we summarize the majority of the large available set of carbon source dependent promoters for protein expression in yeasts, either induced or derepressed by the particular carbon source provided. We examined the most common derepressed promoters for Saccharomyces cerevisiae and other yeasts, and described carbon source inducible promoters and promoters induced by non-sugar carbon sources. A special focus is given to promoters that are activated as soon as glucose is depleted, since such promoters can be very effective and offer an uncomplicated and scalable cultivation procedure.
authors.forename: Katrin
authors.initials: K
authors.lastname: Weinhandl
authors.forename: Margit
authors.initials: M
authors.lastname: Winkler
authors.forename: Anton
authors.initials: A
authors.lastname: Glieder
authors.affiliation: Institute of Molecular Biotechnology, Technical University Graz, Graz, Austria. andrea.camattari@tugraz.at.
authors.forename: Andrea
authors.initials: A
authors.lastname: Camattari
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics / Q000378:metabolism; D002244:Carbon / Q000378:metabolism*; D027501:Monocarboxylic Acid Transporters / Q000235:genetics / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D027981:Symporters / Q000235:genetics / Q000378:metabolism; D043324:beta-Fructofuranosidase / Q000235:genetics / Q000378:metabolism
chemical_list: C118697:JEN1 protein, S cerevisiae; D027501:Monocarboxylic Acid Transporters; D009004:Monosaccharide Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; D027981:Symporters; D002244:Carbon; C509911:ADH2 protein, S cerevisiae; D000426:Alcohol Dehydrogenase; C468437:SUC2 protein, S cerevisiae; D043324:beta-Fructofuranosidase