title: Enhanced biodegradation of total polycyclic aromatic hydrocarbons (TPAHs) by marine halotolerant Achromobacter xylosoxidans using Triton X-100 and β-cyclodextrin--a microcosm approach.
abstract: Ability of Achromobacter xylosoxidans, a chrysene degrading marine halotolerant bacterium to degrade polycyclic aromatic hydrocarbons (PAHs) using a cost effective laboratory microcosm approach, was investigated. Effect of variables as chrysene, glucose as a co-substrate, Triton X-100 as a non-ionic surfactant and β-cyclodextrin as a PAHs solubilizer was examined on degradation of low molecular weight (LMW) and high molecular weight (HMW) PAHs. A total of eleven PAHs detected from polluted saline soil were found to be degraded. Glucose, in combination with Triton X-100 and β-cyclodextrin resulted in 2.8 and 1.4-fold increase in degradation of LMW PAHs and 7.59 and 2.23-fold increase in degradation of HMW PAHs, respectively. Enhanced biodegradation of total PAHs (TPAHs) by amendments with Triton X-100 and β-cyclodextrin using Achromobacter xylosoxidans can prove to be promising approach for in situ bioremediation of marine sites contaminated with PAHs.
authors.affiliation: Department of Life Sciences, Maharaja Krishnakumarsinhji Bhavnagar University, Bhavnagar, Gujarat, India. Electronic address: bpd8256@hotmail.com.
authors.forename: Bharti P
authors.initials: BP
authors.lastname: Dave
authors.affiliation: Department of Life Sciences, Maharaja Krishnakumarsinhji Bhavnagar University, Bhavnagar, Gujarat, India.
authors.forename: Chirag M
authors.initials: CM
authors.lastname: Ghevariya
authors.affiliation: Department of Life Sciences, Maharaja Krishnakumarsinhji Bhavnagar University, Bhavnagar, Gujarat, India.
authors.forename: Jwalant K
authors.initials: JK
authors.lastname: Bhatt
authors.affiliation: Department of Life Sciences, Maharaja Krishnakumarsinhji Bhavnagar University, Bhavnagar, Gujarat, India.
authors.forename: Dushyant R
authors.initials: DR
authors.lastname: Dudhagara
authors.affiliation: Department of Life Sciences, Maharaja Krishnakumarsinhji Bhavnagar University, Bhavnagar, Gujarat, India.
authors.forename: Rahul K
authors.initials: RK
authors.lastname: Rajpara
mesh_terms: D042441:Achromobacter denitrificans / Q000502:physiology*; D001673:Biodegradation, Environmental; D017753:Ecosystem; D052918:Environmental Restoration and Remediation / Q000379:methods*; D017830:Octoxynol / Q000737:chemistry*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D055049:Salt Tolerance; D013501:Surface-Active Agents / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D013501:Surface-Active Agents; D014874:Water Pollutants, Chemical; D047392:beta-Cyclodextrins; D017830:Octoxynol; C031215:betadex
keywords: Achromobacter xylosoxidans; Bioremediation; Microcosm; Triton X-100; β-cyclodextrin