title: Bone and cartilage repair by transplantation of induced pluripotent stem cells in murine joint defect model.
abstract: The establishment of cartilage regenerative medicine has been an important issue in the clinical field, because cartilage has the poor ability of self-repair. Currently, tissue engineering using autologous chondrocytes has risen, but we should investigate more appropriate cell sources that can be obtained without any quantitative limitation. In this study, we focused on induced pluripotent stem (iPS) cells, in which the ethical hurdle does not seem higher than that of embryonic stem cells. Mouse iPS cells were transplanted into the mouse joint defect model of the knee. Strains of the transplants and hosts were arranged to be either closest (homology 75% in genetic background) or identical (100%). For transplantation, we embedded the iPS cells within the collagen hydrogel in order to obtain the effective administration of the cells into defects, which induced the differentiation of the iPS cells. At 8 weeks of transplantation, although the iPS cells with a 75% homology to the host in the genetic background tended to form teratoma, those of 100% showed a joint regeneration. GFP immunohistochemistry proved that the transplanted iPS cells were responsible for the bone and cartilage repair. Taking these results together, the iPS cells are regarded as a promising cell source for the cartilage tissue engineering.
authors.affiliation: Department of Cartilage & Bone Regeneration (Fujisoft) Graduate Schoolof Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Sakura
authors.initials: S
authors.lastname: Uto
authors.forename: Satoru
authors.initials: S
authors.lastname: Nishizawa
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Takasawa
authors.forename: Yukiyo
authors.initials: Y
authors.lastname: Asawa
authors.forename: Yuko
authors.initials: Y
authors.lastname: Fujihara
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Takato
authors.forename: Kazuto
authors.initials: K
authors.lastname: Hoshi
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001861:Bone Regeneration / Q000502:physiology*; D002356:Cartilage / Q000473:pathology*; D002454:Cell Differentiation; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D003094:Collagen / Q000737:chemistry; D015870:Gene Expression; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006650:Histocompatibility Testing; D020100:Hydrogels; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D007718:Knee Injuries / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D007719:Knee Joint / Q000473:pathology*; D051379:Mice; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D013724:Teratoma / Q000473:pathology; D014945:Wound Healing / Q000502:physiology*
chemical_list: D015415:Biomarkers; D020100:Hydrogels; D049452:Green Fluorescent Proteins; D003094:Collagen