title: Mass spectrometry as an efficient tool for the characterization of amyloid β peptide 25-35 self-assembly species in aggregation and inhibition studies.
abstract: Amyloid beta 25-35 [Aβ (25-35)], as a peptide model for full-length Aβ in structural and functional investigations, has been chosen for aggregation studies. The complexity of the Aβ (25-35) aggregation process required a multi-methodological analytical approach to obtain reliable and reproducible results. Here, we describe the results obtained by the use of mass spectrometry (MS) for the structural characterization of the self-assembly species during the aggregation process and for the definition of the self-assembly kinetics and myricetin inhibition patterns, comparing the results with those obtained by using the well-established spectroscopic method based on thioflavin T fluorescence. Flow injection electrospray ionization-ion trap-mass spectrometry (ESI-IT-MS) was applied to monitor the disappearance of the monomer specie in the first steps, whereas matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-ToF-MS) was used to follow monomer and small oligomer self-assembly trends in the early stages of the nucleating process.
authors.affiliation: Department of Pharmacy and Biotechnology, Alma Mater Studiorum-University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy.
authors.forename: J
authors.initials: J
authors.lastname: Fiori
authors.affiliation: Interdepartmental Centre for Biotechnology Research (CIRB), Alma Mater Studiorum-University of Bologna, Via S, Donato 15, 40127 Bologna, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Naldi
authors.affiliation: Department for Life Quality Studies, Alma Mater Studiorum-University of Bologna, Rimini Campus, Corso di Augusto 237, 47921 Rimini, Italy. vincenza.andrisano@unibo.it.
authors.forename: V
authors.initials: V
authors.lastname: Andrisano
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism*; D005419:Flavonoids / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008956:Models, Chemical; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods* / Q000592:standards*; D013329:Structure-Activity Relationship
chemical_list: D016229:Amyloid beta-Peptides; D005419:Flavonoids; C040015:myricetin