title: MiR-143 suppresses osteogenic differentiation by targeting Osterix.
abstract: Osterix (Osx) is an essential transcription factor for osteoblast differentiation and bone formation. However, the knowledge of the regulation of Osx expression is poor. MicroRNAs (miRNAs), a class of small non-coding RNAs, play critical roles in numerous biological processes, including the proliferation, differentiation, and survival of cells and organisms. Herein, we aimed to explore the effect of miR-143 on Osx expression and osteogenic differentiation. miR-143, which was suppressor of the osteogenic differentiation of MC3T3-E1 cells, had decreased levels of expression during osteogenic differentiation. Moreover, Osx was identified to be a direct target of miR-143. Inhibition of Osx performed similar effect with miR-143 on osteogenic differentiation, while overexpression of Osx could partially reverse the suppressive effect of miR-143. Collectively, these data indicate that miR-143 is a novel regulator of Osx, and it might play an essential role in the regulation of osteogenic differentiation.
authors.affiliation: Department of Orthopaedic Trauma, TianJin Hospital, Jiefang Road No. 406, Hexi District, Tianjin, 300211, China, enqi_litj@163.com.
authors.forename: Enqi
authors.initials: E
authors.lastname: Li
authors.forename: Jinli
authors.initials: J
authors.lastname: Zhang
authors.forename: Tianxiang
authors.initials: T
authors.lastname: Yuan
authors.forename: Baotong
authors.initials: B
authors.lastname: Ma
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D010006:Osteoblasts / Q000378:metabolism; D010012:Osteogenesis; D000076182:Sp7 Transcription Factor; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D035683:MicroRNAs; C532884:MIRN143 microRNA, mouse; D000076182:Sp7 Transcription Factor; C479466:Sp7 protein, mouse; D014157:Transcription Factors