title: Effect of novel chitosan-fluoroaluminosilicate resin modified glass ionomer cement supplemented with translationally controlled tumor protein on pulp cells.
abstract: Dental materials that can promote cell proliferation and function is required for regenerative pulp therapy. Resin modified glass ionomer cement (RMGIC), a broadly used liner or restorative material, can cause apoptosis to pulp cells mainly due to HEMA (2-hydroxyethyl methacrylate), the released residual monomer. Recent studies found that chitosan and albumin could promote release of protein in GIC while translationally controlled tumor protein (TCTP) has an anti-apoptotic activity against HEMA. The aim of this study was to examine the effect of chitosan and albumin modified RMGIC (Exp-RMGIC) supplemented with TCTP on pulp cell viability and mineralization. Exp-RMGIC+TCTP was composed of RMGIC powder incorporated with 15 % of chitosan, 5 % albumin and supplemented with TCTP mixed with the same liquid components of RMGIC. The effect of each specimen on pulp cells was examined using the Transwell plate. From the MTT assay, Exp-RMGIC+TCTP had the highest percentages of viable cells (P < 0.05) at both 24 and 74 h. Flow cytometry revealed that, after 24 h, Exp-RMGIC+TCTP gave the lowest percentages of apoptotic cells compared to other groups. There was no difference in alkaline phosphatase (ALP) activity among different formula of the specimens, while cells cultured in media with TCTP had higher ALP activity. Von Kossa staining revealed that RMGIC+TCTP, and Exp-RMGIC+TCTP had higher percentages of calcium deposit area compared to those without TCTP. It was concluded that Exp-RMGIC supplemented with TCTP had less cytotoxicity than RMGIC and can protect cells from apoptosis better than RMGIC supplemented with TCTP.
authors.affiliation: Department of Oral Biology and Occlusion, Faculty of Dentistry, Prince of Songkla University, Hat Yai, 90112, Songkhla, Thailand.
authors.forename: Nattaporn
authors.initials: N
authors.lastname: Wanachottrakul
authors.forename: Wilaiwan
authors.initials: W
authors.lastname: Chotigeat
authors.forename: Ureporn
authors.initials: U
authors.lastname: Kedjarune-Leggat
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D017607:Aluminum Compounds / Q000737:chemistry*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D014408:Biomarkers, Tumor / Q000008:administration & dosage* / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D003782:Dental Pulp / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology; D005459:Fluorides / Q000737:chemistry*; D005899:Glass Ionomer Cements / Q000737:chemistry* / Q000633:toxicity; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry / Q000633:toxicity; D033561:Penaeidae / Q000235:genetics; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics; D012038:Regeneration / Q000187:drug effects; D017655:Silicon Compounds / Q000737:chemistry*; D000090324:Tumor Protein, Translationally-Controlled 1
chemical_list: D017607:Aluminum Compounds; D014408:Biomarkers, Tumor; D005899:Glass Ionomer Cements; D008689:Methacrylates; D011994:Recombinant Proteins; D017655:Silicon Compounds; C000715647:TPT1 protein, human; D000090324:Tumor Protein, Translationally-Controlled 1; C005044:hydroxyethyl methacrylate; D048271:Chitosan; C020328:fluoroaluminum silicate; D000469:Alkaline Phosphatase; D005459:Fluorides