title: Efficient sample preparation in immuno-matrix-assisted laser desorption/ionization mass spectrometry using acoustic trapping.
abstract: Acoustic trapping of minute bead amounts against fluid flow allows for easy automation of multiple assay steps, using a convenient aspirate/dispense format. Here, a method based on acoustic trapping that allows sample preparation for immuno-matrix-assisted laser desorption/ionization mass spectrometry using only half a million 2.8 μm antibody covered beads is presented. The acoustic trapping is done in 200 × 2000 μm(2) glass capillaries and provides highly efficient binding and washing conditions, as shown by complete removal of detergents and sample processing times of 5-10 min. The versatility of the method is demonstrated using an antibody against Angiotensin I (Ang I), a peptide hormone involved in hypotension. Using this model system, the acoustic trapping was efficient in enriching Angiotensin at 400 pM spiked in plasma samples.
authors.affiliation: Department of Measurement Technology and Industrial Electrical Engineering, Lund University, Lund, Sweden.
authors.forename: Björn
authors.initials: B
authors.lastname: Hammarström
authors.affiliation: Department of Measurement Technology and Industrial Electrical Engineering, Lund University, Lund, Sweden.
authors.forename: Hong
authors.initials: H
authors.lastname: Yan
authors.affiliation: Department of Measurement Technology and Industrial Electrical Engineering, Lund University, Lund, Sweden.
authors.forename: Johan
authors.initials: J
authors.lastname: Nilsson
authors.affiliation: Department of Measurement Technology and Industrial Electrical Engineering, Lund University, Lund, Sweden ; Lund University, CREATE Health, Lund, Sweden.
authors.forename: Simon
authors.initials: S
authors.lastname: Ekström