title: Cell-free protein synthesis in microfluidic 96-well plates.
abstract: Cell-free protein synthesis (CFPS) enables rapid protein expression for the structural and functional characterization of proteins. Implementation of CFPS in a microfluidic platform has additional benefits such as reduced reaction volumes and simultaneous expression of multiple proteins. Here, we describe a microfluidic device that is composed of 96 continuous-exchange cell-free protein expression units and produces a protein synthesis yield up to 87 times higher than a conventional batch system.
authors.affiliation: Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Jackson
authors.forename: Ruba
authors.initials: R
authors.lastname: Khnouf
authors.forename: Z Hugh
authors.initials: ZH
authors.lastname: Fan
mesh_terms: D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D014176:Protein Biosynthesis; D013698:Templates, Genetic; D013997:Time Factors