title: Reproducibility of measuring cerebral blood flow by laser-Doppler flowmetry in mice.
abstract: Laser-Doppler flowmetry has been widely used to trace hemodynamic changes in experimental stroke research. The purpose of the present study was to evaluate the day-to-day test-retest reproducibility of measuring cerebral blood flow by LDF in awake mice. The flux indicating cerebral blood flow (CBF), red blood cell (RBC) velocity, and RBC concentration were measured with LDF via cranial windows for the bilateral somatosensory cortex in awake mice. LDF measurements were performed three times, at baseline, 1 hour after, and 7 days after the baseline measurement. Moreover, breathing rate (BR) and partial pressure of transcutaneous CO₂ (PtCO₂) were measured simultaneously with LDF measurement. Intraclass correlation coefficient (ICC) and within-subject coefficient of variation (CVw) were calculated. CBF, RBC velocity, and RBC concentration showed good day-to-day test-retest reproducibility (ICC: 0.61 - 0.95, CVw: 8.3% - 15.4%). BR and PtCO₂ in awake mice were stable during the course of the experiments. The evaluation of cerebral microcirculation using LDF appears to be applicable to long-term studies.
authors.affiliation: Biophysics Program, Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Tajima
authors.affiliation: Biophysics Program, Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takuwa
authors.affiliation: Biophysics Program, Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawaguchi
authors.affiliation: Biophysics Program, Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Kazuto
authors.initials: K
authors.lastname: Masamoto
authors.affiliation: Biophysics Program, Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ikoma
authors.affiliation: Biophysics Program, Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Chie
authors.initials: C
authors.lastname: Seki
authors.affiliation: Biophysics Program, Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Taniguchi
authors.affiliation: Biophysics Program, Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Iwao
authors.initials: I
authors.lastname: Kanno
authors.affiliation: Department of Neurological Surgery, Chiba University Graduate School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
authors.forename: Naokatsu
authors.initials: N
authors.lastname: Saeki
authors.affiliation: Biophysics Program, Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ito
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001785:Blood Gas Monitoring, Transcutaneous / Q000379:methods; D002245:Carbon Dioxide / Q000097:blood; D054022:Cerebrum / Q000098:blood supply*; D017078:Laser-Doppler Flowmetry / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010313:Partial Pressure; D012039:Regional Blood Flow / Q000502:physiology*; D015203:Reproducibility of Results; D056152:Respiratory Rate
chemical_list: D002245:Carbon Dioxide