title: Use of larder beetles (Coleoptera: Dermestidae) to deflesh human jaws.
abstract: We describe new experimental data for the defleshing of human bones using larder beetles (Dermestes haemorrhoidalis) (Küster, 1852). Although the ability of larder beetles to feed on vertebrate remains has been, and still is, used by taxidermists to deflesh skulls and bones, this method has never been documented from a quantitative perspective and has over time become ignored in most forensic anthropology or odontology laboratories. To promote the rational and efficient use of this method, we performed experiments to estimate the quantity of food consumed by larvae. From the 2nd instar to nymphosis, each larva consumed a mean of 0.13±0.03 g of dry beef muscle. We then used 100±50 D. haemorrhoidalis adults and 100±50 larvae to deflesh human maxillae and mandibles sampled within a forensic context (victim identification). Each sample was weighed and photographed before, during and after the experiment. According to our experiments, 20-25 days were sufficient to completely deflesh all of the samples. We concluded that a small number of larder beetles can be used to efficiently deflesh human jaws. According to this result, the use of larder beetles appears to be an inexpensive, simple and efficient way to clean mandibles and maxillae. Furthermore, this method is DNA-safe (compared to usual maceration techniques) and thus allows the samples to be used for subsequent DNA and drug analyses.
authors.affiliation: Univ Lille Nord de France, Lille F-59000 France; UDSL, Forensic Taphonomy Unit, Lille F-59000, France. Electronic address: damien@forenseek.org.
authors.forename: D
authors.initials: D
authors.lastname: Charabidze
authors.affiliation: Univ Lille Nord de France, Lille F-59000 France; UDSL, Forensic Taphonomy Unit, Lille F-59000, France.
authors.forename: T
authors.initials: T
authors.lastname: Colard
authors.affiliation: Univ Lille Nord de France, Lille F-59000 France; UDSL, Forensic Taphonomy Unit, Lille F-59000, France.
authors.forename: A
authors.initials: A
authors.lastname: Becart
authors.affiliation: Univ Lille Nord de France, Lille F-59000 France; UDSL, Forensic Taphonomy Unit, Lille F-59000, France.
authors.forename: V
authors.initials: V
authors.lastname: Hedouin
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001517:Coleoptera / Q000502:physiology*; D005247:Feeding Behavior / Q000502:physiology*; D005260:Female; D018732:Forensic Anthropology / Q000379:methods; D006801:Humans; D007814:Larva; D008297:Male; D008334:Mandible / Q000473:pathology*; D008437:Maxilla / Q000473:pathology*; D008875:Middle Aged; D010781:Photography; D055815:Young Adult
keywords: Bones cleaning; Dermestes haemorrhoidalis; Forensic entomology; Odontology; Victim identification