title: Dietary-suppression of hepatic lipogenic enzyme expression in intact male transgenic mice.
abstract: OBJECTIVE
  To study, in intact male transgenic mice, the effects of three diets based on olive oil and olive oil diet supplemented with lovastatin and orlistat on hepatic lipogenic enzymes expression, considered markers of cell proliferation.
  
  
  METHODS
  Forty Apc(Min/+) mice were randomly divided into 4 groups and fed for 10 wk: olive oil (OO) group, n = 10 animals received a diet with olive oil 12%; olive oil plus lovastatin (LOVA) group, n = 10 animals received the same diet with olive oil supplemented with lovastatin 5 mg/kg; olive oil plus orlistat (OR) group, n = 10 animals fed the diet with olive oil supplemented with orlistat 50 mg/kg and SD group, n = 10 animals fed a standard diet. The activity of lipogenic enzymes and their gene expression were evaluated by radiometric and real-time reverse transcription-polymerase chain reaction assay, respectively.
  
  
  RESULTS
  After 10 wk of dietary treatment, the body weight was no different among animal groups (21.3 ± 3.1 g for standard group, 22.1 ± 3.6 g for OO group, 22.0 ± 3.2 g for LOVA group and 20.7 ± 3.4 g for OR group, data expressed as mean ± SD), observing a generalized well-being in all animals. All the dietary managed treated groups presented significantly reduced hepatic levels of fatty acid synthase, farnesyl pyrophosphate synthase and 3-hydroxyl-3-methyl-glutaryl CoA reductase activity and gene expression when compared with the mice fed the standard diet. To evaluate cell proliferation in the liver of treated mice, the levels of cyclin E mRNA have been measured, demonstrating a significant reduction of cyclin E gene expression in all treated groups. Evidence of reduced hepatic cell proliferation was present overall in OO group mice.
  
  
  CONCLUSIONS
  We confirm the role of lipogenic enzymes as markers of cell proliferation, suggesting that appropriate dietary management alone or with drugs can be a feasible approach to counteract hepatic cell proliferation in mice.
authors.affiliation: Maria Notarnicola, Maria Gabriella Caruso, Valeria Tutino, Laboratory of Nutritional Biochemistry, National Institute for Digestive Diseases, Castellana Grotte, 70013 Bari, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Notarnicola
authors.affiliation: Maria Notarnicola, Maria Gabriella Caruso, Valeria Tutino, Laboratory of Nutritional Biochemistry, National Institute for Digestive Diseases, Castellana Grotte, 70013 Bari, Italy.
authors.forename: Maria Gabriella
authors.initials: MG
authors.lastname: Caruso
authors.affiliation: Maria Notarnicola, Maria Gabriella Caruso, Valeria Tutino, Laboratory of Nutritional Biochemistry, National Institute for Digestive Diseases, Castellana Grotte, 70013 Bari, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Tafaro
authors.affiliation: Maria Notarnicola, Maria Gabriella Caruso, Valeria Tutino, Laboratory of Nutritional Biochemistry, National Institute for Digestive Diseases, Castellana Grotte, 70013 Bari, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Tutino
authors.affiliation: Maria Notarnicola, Maria Gabriella Caruso, Valeria Tutino, Laboratory of Nutritional Biochemistry, National Institute for Digestive Diseases, Castellana Grotte, 70013 Bari, Italy.
authors.forename: Giusy
authors.initials: G
authors.lastname: Bianco
authors.affiliation: Maria Notarnicola, Maria Gabriella Caruso, Valeria Tutino, Laboratory of Nutritional Biochemistry, National Institute for Digestive Diseases, Castellana Grotte, 70013 Bari, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Minoia
authors.affiliation: Maria Notarnicola, Maria Gabriella Caruso, Valeria Tutino, Laboratory of Nutritional Biochemistry, National Institute for Digestive Diseases, Castellana Grotte, 70013 Bari, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Francavilla
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation; D019927:Cyclin E / Q000235:genetics / Q000378:metabolism; D004041:Dietary Fats / Q000378:metabolism*; D015536:Down-Regulation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D054890:Fatty Acid Synthase, Type I / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D017491:Genes, APC; D005838:Genotype; D051228:Geranyltranstransferase / Q000235:genetics / Q000378:metabolism; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics / Q000378:metabolism; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology; D007783:Lactones / Q000494:pharmacology; D050155:Lipogenesis / Q000187:drug effects / Q000235:genetics*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008148:Lovastatin / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D000069463:Olive Oil; D000077403:Orlistat; D010641:Phenotype; D010938:Plant Oils / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D015415:Biomarkers; D019927:Cyclin E; D004041:Dietary Fats; D004791:Enzyme Inhibitors; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D007783:Lactones; D000069463:Olive Oil; D010938:Plant Oils; D012333:RNA, Messenger; D000077403:Orlistat; D008148:Lovastatin; D006903:Hydroxymethylglutaryl CoA Reductases; D054890:Fatty Acid Synthase, Type I; D051228:Geranyltranstransferase
keywords: Dietary treatment; Lipogenic enzymes; Liver; Markers of cell proliferation; Transgenic mice