title: Comparative effects of biodynes, tocotrienol-rich fraction, and tocopherol in enhancing collagen synthesis and inhibiting collagen degradation in stress-induced premature senescence model of human diploid fibroblasts.
abstract: Biodynes, tocotrienol-rich fraction (TRF), and tocopherol have shown antiaging properties. However, the combined effects of these compounds on skin aging are yet to be investigated. This study aimed to elucidate the skin aging effects of biodynes, TRF, and tocopherol on stress-induced premature senescence (SIPS) model of human diploid fibroblasts (HDFs) by determining the expression of collagen and MMPs at gene and protein levels. Primary HDFs were treated with biodynes, TRF, and tocopherol prior to hydrogen peroxide (H2O2) exposure. The expression of COL1A1, COL3A1, MMP1, MMP2, MMP3, and MMP9 genes was determined by qRT-PCR. Type I and type III procollagen proteins were measured by Western blotting while the activities of MMPs were quantified by fluorometric Sensolyte MMP Kit. Our results showed that biodynes, TRF, and tocopherol upregulated collagen genes and downregulated MMP genes (P < 0.05). Type I procollagen and type III procollagen protein levels were significantly increased in response to biodynes, TRF, and tocopherol treatment (P < 0.05) with reduction in MMP-1, MMP-2, MMP-3, and MMP-9 activities (P < 0.05). These findings indicated that biodynes, TRF, and tocopherol effectively enhanced collagen synthesis and inhibited collagen degradation and therefore may protect the skin from aging.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
authors.forename: Suzana
authors.initials: S
authors.lastname: Makpol
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
authors.forename: Faidruz Azura
authors.initials: FA
authors.lastname: Jam
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
authors.forename: Shy Cian
authors.initials: SC
authors.lastname: Khor
authors.affiliation: R&D Plantation and Agri-Business Division, Sime Darby Research Sdn Bhd, 42960 Carey Island, Selangor, Malaysia.
authors.forename: Zahariah
authors.initials: Z
authors.lastname: Ismail
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
authors.forename: Yasmin Anum
authors.initials: YA
authors.lastname: Mohd Yusof
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
authors.forename: Wan Zurinah Wan
authors.initials: WZ
authors.lastname: Ngah
mesh_terms: D016922:Cellular Senescence / Q000187:drug effects*; D002648:Child; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D004171:Diploidy; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008297:Male; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D059748:Proteolysis / Q000187:drug effects*; D013312:Stress, Physiological / Q000187:drug effects*; D024505:Tocopherols / Q000494:pharmacology*; D024508:Tocotrienols / Q000494:pharmacology*
chemical_list: D024508:Tocotrienols; D003094:Collagen; D020782:Matrix Metalloproteinases; D024505:Tocopherols