title: Erythroid-extrinsic regulation of normal erythropoiesis by retinoic acid receptors.
abstract: Vitamin A and its derivatives (retinoids) are important regulators of haematopoiesis, acting via retinoic acid receptors (RARs). Epidemiological studies indicated an association of vitamin A deficiency with anaemia in humans. To define the requirements of RARs in erythropoiesis, we evaluated erythroid parameters in RAR germ-line deficient and conditional knock out mice with erythroid specific deletion of RARs. Adult RARγ(-/-) mice were anaemic, however, Epor-Cre Rara(fl/fl) , Epor-Cre Rarg(fl/fl) and Epor-Cre Rara(fl/fl) g(fl/fl) mice were normal, indicating a lack of an erythroid intrinsic RAR function. Therefore, erythroid-specific RAR function is dispensable for erythropoiesis and RARγ plays an erythroid extrinsic role in erythropoiesis.
authors.affiliation: Department of Medicine at St. Vincent's Hospital, St. Vincent's Institute of Medical Research, University of Melbourne, Fitzroy, Vic., Australia.
authors.forename: Sita R
authors.initials: SR
authors.lastname: Dewamitta
authors.forename: Chacko
authors.initials: C
authors.lastname: Joseph
authors.forename: Louise E
authors.initials: LE
authors.lastname: Purton
authors.forename: Carl R
authors.initials: CR
authors.lastname: Walkley
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D004900:Erythroblasts / Q000378:metabolism; D004920:Erythropoiesis / Q000502:physiology*; D016130:Immunophenotyping; D051379:Mice; D018345:Mice, Knockout; D010641:Phenotype; D017467:Receptors, Erythropoietin / Q000235:genetics; D018168:Receptors, Retinoic Acid / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D000072482:Retinoic Acid Receptor alpha; D000097768:Retinoic Acid Receptor gamma
chemical_list: D017467:Receptors, Erythropoietin; D018168:Receptors, Retinoic Acid; D000072482:Retinoic Acid Receptor alpha; D000097768:Retinoic Acid Receptor gamma; C000607071:RARA protein, human; C000607072:Rara protein, mouse
keywords: anaemia; erythroid development; experimental haematology; retinoic acid; vitamin A