title: Effect of neurosteroid modulation on global ischaemia-reperfusion-induced cerebral injury in mice.
abstract: The present study was designed to investigate the putative effect of neurosteroid modulation on global ischaemia-reperfusion-induced cerebral injury in mice. Bilateral carotid artery occlusion followed by reperfusion, produced a significant rise in cerebral infarct size along with impairment of grip strength and motor coordination in Swiss albino mice. Administration of carbamazepine (16 mg/kg, i.p.) before global cerebral ischaemia significantly attenuated cerebral infarct size and improved the motor performance. However, administration of indomethacin (100 mg/kg, i.p.) attenuated the neuroprotective effect of carbamazepine. Mexiletine (50 mg/kg, i.p.) did not produce significant neuroprotective effect. It may be concluded that the neuroprotective effect of carbamazepine may be due to increase in synthesis of neurosteroids perhaps by activating enzyme (3α HSD) as indomethacin attenuated the neuroprotective effect of carbamazepine. The sodium channel blocking effect of carbamazepine may not be involved in neuroprotection as mexiletine, a sodium channel blocker, did not produce significant neuroprotective effect.
authors.affiliation: Rayat and Bahra Institute of Pharmacy, Sahauran, Mohali-140104, Punjab, India.
authors.forename: Amarjot Kaur
authors.initials: AK
authors.lastname: Grewal
authors.affiliation: Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala-147002, Punjab, India.
authors.forename: Amteshwar Singh
authors.initials: AS
authors.lastname: Jaggi
authors.affiliation: Rayat and Bahra Institute of Pharmacy, Sahauran, Mohali-140104, Punjab, India.
authors.forename: Avtar Chand
authors.initials: AC
authors.lastname: Rana
authors.affiliation: Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala-147002, Punjab, India.
authors.forename: Nirmal
authors.initials: N
authors.lastname: Singh
keywords: Carbamazepine; Global-ischaemia-reperfusion injury; Indomethacin; Mexiletine; Neurosteroids