title: The gene expression profile of peripheral blood mononuclear cells from EV71-infected rhesus infants and the significance in viral pathogenesis.
abstract: Enterovirus 71 (EV71) is the major pathogen responsible for fatal hand, foot and mouth disease (HFMD). Our previous work reported on an EV71-infected rhesus monkey infant model that presented with histo-pathologic changes of the central nervous system (CNS) and lungs. This study is focused on the correlated modulation of gene expression in the peripheral blood mononuclear cells (PBMCs) from EV71-infected rhesus monkey infants. The expression of more than 500 functional genes associated with multiple pathways was modulated. The expression of genes associated with immune inflammatory responses was up-regulated during the period from days 4 to 10 post-infection. The expression of two genes (TAC1 and IL17A), which play major roles in inflammatory reactions, was remarkably up-regulated during the infection period. Furthermore, a higher expression level of the TAC1 gene was identified in the CNS compared to the lungs, but a high expression level of the IL-17A gene was observed in the lungs and not in the CNS. The results of this study suggest at least two facts about EV71 infection, which are that: the TAC1 gene that encodes substance P and neurokinin-A is present in both PBMCs and the hypothalamus; and the up-regulation of IL-17A is sustained in the peripheral blood.
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medicine Science and Peking Union Medical College, Kunming, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medicine Science and Peking Union Medical College, Kunming, China.
authors.forename: Erxia
authors.initials: E
authors.lastname: Yang
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medicine Science and Peking Union Medical College, Kunming, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Pu
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medicine Science and Peking Union Medical College, Kunming, China.
authors.forename: Longding
authors.initials: L
authors.lastname: Liu
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medicine Science and Peking Union Medical College, Kunming, China.
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Che
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medicine Science and Peking Union Medical College, Kunming, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medicine Science and Peking Union Medical College, Kunming, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medicine Science and Peking Union Medical College, Kunming, China.
authors.forename: Lichun
authors.initials: L
authors.lastname: Wang
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medicine Science and Peking Union Medical College, Kunming, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Ding
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medicine Science and Peking Union Medical College, Kunming, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhao
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medicine Science and Peking Union Medical College, Kunming, China.
authors.forename: Na
authors.initials: N
authors.lastname: Ma
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medicine Science and Peking Union Medical College, Kunming, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Song
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medicine Science and Peking Union Medical College, Kunming, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medicine Science and Peking Union Medical College, Kunming, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Shen
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medicine Science and Peking Union Medical College, Kunming, China.
authors.forename: Donghong
authors.initials: D
authors.lastname: Tang
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medicine Science and Peking Union Medical College, Kunming, China.
authors.forename: Hongtai
authors.initials: H
authors.lastname: Huang
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medicine Science and Peking Union Medical College, Kunming, China.
authors.forename: Zhixiao
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Bioinformatic analyses, Novel Bioinformatics Co., Ltd, Shanghai, China.
authors.forename: Dai
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Bioinformatic analyses, Novel Bioinformatics Co., Ltd, Shanghai, China.
authors.forename: Mingfei
authors.initials: M
authors.lastname: Feng
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medicine Science and Peking Union Medical College, Kunming, China.
authors.forename: Qihan
authors.initials: Q
authors.lastname: Li
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000378:metabolism / Q000821:virology; D016000:Cluster Analysis; D029821:Enterovirus A, Human / Q000502:physiology*; D004769:Enterovirus Infections / Q000235:genetics* / Q000276:immunology / Q000821:virology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006232:Hand, Foot and Mouth Disease / Q000235:genetics / Q000276:immunology / Q000821:virology; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000276:immunology; D020381:Interleukin-17 / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008253:Macaca mulatta; D059467:Transcriptome
chemical_list: D020381:Interleukin-17