title: Antifibrotic effect of pirfenidone on human pterygium fibroblasts.
abstract: OBJECTIVE
  The effects of pirfenidone were investigated on cultured human pterygium fibroblasts (HPFs).
  
  
  METHODS
  HPFs were obtained from pterygium surgery and subjected to primary culture. After treatment with 0.5, 1.0 or 1.5 mg/mL pirfenidone, MTT and cell migration assays were performed, and procollagen secretion and TGF-β expression were measured by Western blotting and immunofluorescence analysis.
  
  
  RESULTS
  Pirfenidone had a significant inhibitory effect on HPF proliferation, migration and collagen synthesis. There were no differences between the cells treated with 0.5, 1.0 and 1.5 mg/mL pirfenidone and the controls in the MTT assay. After 48 h of treatment with 1.0 or 1.5 mg/mL pirfenidone, TGF-β expression was significantly decreased.
  
  
  CONCLUSIONS
  These findings demonstrate that pirfenidone inhibits the proliferation, migration and procollagen secretion of HPFs at nontoxic concentrations by decreasing TGF-β expression. Thus, pirfenidone may be considered as a safe adjuvant for pterygium surgery to prevent recurrence.
authors.affiliation: Department of Ophthalmology, Ajou University School of Medicine , Suwon , Republic of Korea.
authors.forename: Kihwang
authors.initials: K
authors.lastname: Lee
authors.forename: Sun
authors.initials: S
authors.lastname: Young Lee
authors.forename: So Yean
authors.initials: SY
authors.lastname: Park
authors.forename: Hongseok
authors.initials: H
authors.lastname: Yang
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D015153:Blotting, Western; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D011347:Procollagen / Q000096:biosynthesis* / Q000187:drug effects; D011625:Pterygium / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D011728:Pyridones / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000096:biosynthesis
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D011347:Procollagen; D011728:Pyridones; D014409:Tumor Necrosis Factor-alpha; C093844:pirfenidone
keywords: Antifibrotic effect; TGF-beta; fibroblast; pirfenidone; pterygium