title: XIAP inhibitor and antiestrogen embelin abrogates metastasis and augments apoptosis in estrogen receptor positive human breast adenocarcinoma cell line MCF-7.
abstract: Tamoxifen therapy for the treatment of hormone responsive breast cancer has limitations due to acquired resistance in the case of recurrences. Embelin, a known inhibitor of X-linked inhibitor of apoptosis protein (XIAP) was also reported to exhibit strong antiestrogenic effects in animal models. Dual role of embelin as a proapoptotic and antiestrogenic agent may have potential benefits in the therapy of breast cancer. In this study, the effects of embelin treatment on estrogen receptor positive Human breast adenocarcinoma (MCF-7) cells was investigated to primarily understand if embelin being an antiestrogen and XIAP inhibitor could be a potential alternative to tamoxifen therapy. Results revealed that, embelin at a concentration of 65 μg/ml attenuated proliferation, inhibited metastatic migration, modulated the expression of Bcl2, Caspases and induced apoptosis in MCF-7 cells which was found to be p53 mediated. Hence, chemotherapy with embelin could be a promising strategy to be experimented in hormone responsive breast cancers.
authors.affiliation: Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi Campus, Bangalore, 560056, Karnataka, India.
authors.forename: K R
authors.initials: KR
authors.lastname: Sumalatha
authors.forename: G
authors.initials: G
authors.lastname: Abiramasundari
authors.forename: G K
authors.initials: GK
authors.lastname: Chetan
authors.forename: T
authors.initials: T
authors.lastname: Divya
authors.forename: G
authors.initials: G
authors.lastname: Sudhandiran
authors.forename: M
authors.initials: M
authors.lastname: Sreepriya
mesh_terms: D000230:Adenocarcinoma; D017209:Apoptosis / Q000187:drug effects; D016227:Benzoquinones / Q000494:pharmacology*; D049109:Cell Proliferation; D020847:Estrogen Receptor Modulators / Q000494:pharmacology*; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D009362:Neoplasm Metastasis / Q000188:drug therapy*; D011960:Receptors, Estrogen / Q000378:metabolism; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000037:antagonists & inhibitors / Q000235:genetics*
chemical_list: D016227:Benzoquinones; D020847:Estrogen Receptor Modulators; D011960:Receptors, Estrogen; D051636:X-Linked Inhibitor of Apoptosis Protein; C496866:XIAP protein, human; C010945:embelin