title: Donor age negatively impacts adipose tissue-derived mesenchymal stem cell expansion and differentiation.
abstract: BACKGROUND
  Human adipose tissue is an ideal autologous source of mesenchymal stem cells (MSCs) for various regenerative medicine and tissue engineering strategies. Aged patients are one of the primary target populations for many promising applications. It has long been known that advanced age is negatively correlated with an organism's reparative and regenerative potential, but little and conflicting information is available about the effects of age on the quality of human adipose tissue derived MSCs (hAT-MSCs).
  
  
  METHODS
  To study the influence of age, the expansion and in vitro differentiation potential of hAT-MSCs from young (<30 years), adult (35-50 years) and aged (>60 years) individuals were investigated. MSCs were characterized for expression of the genes p16(INK4a) and p21 along with measurements of population doublings (PD), superoxide dismutase (SOD) activity, cellular senescence and differentiation potential.
  
  
  RESULTS
  Aged MSCs displayed senescent features when compared with cells isolated from young donors, concomitant with reduced viability and proliferation. These features were also associated with significantly reduced differentiation potential in aged MSCs compared to young MSCs.
  
  
  CONCLUSIONS
  In conclusion, advancing age negatively impacts stem cell function and such age related alterations may be detrimental for successful stem cell therapies.
authors.forename: Mahmood S
authors.initials: MS
authors.lastname: Choudhery
authors.forename: Michael
authors.initials: M
authors.lastname: Badowski
authors.forename: Angela
authors.initials: A
authors.lastname: Muise
authors.forename: John
authors.initials: J
authors.lastname: Pierce
authors.affiliation: Department of Immunobiology, College of Medicine, The University of Arizona, PO Box 245221, 85724, Tucson, AZ, USA. davidh@email.arizona.edu.
authors.forename: David T
authors.initials: DT
authors.lastname: Harris
mesh_terms: D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D000367:Age Factors; D000368:Aged; D000375:Aging / Q000502:physiology*; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002469:Cell Separation; D048430:Cell Shape; D002470:Cell Survival; D016922:Cellular Senescence; D020219:Chondrogenesis; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000201:enzymology; D008875:Middle Aged; D009474:Neurons / Q000166:cytology; D010012:Osteogenesis; D010641:Phenotype; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000378:metabolism; D014019:Tissue Donors
chemical_list: D013482:Superoxide Dismutase