title: Implementation of Fourier transform infrared spectroscopy for the rapid typing of uropathogenic Escherichia coli.
abstract: In this paper, we demonstrate that Fourier transform infrared (FT-IR) spectroscopy is able to discriminate rapidly between uropathogenic Escherichia coli (UPEC) of key lineages with only relatively simple sample preparation. A total of 95 bacteria from six different epidemiologically important multilocus sequence types (ST10, ST69, ST95, ST73, ST127 and ST131) were used in this project and principal component-discriminant function analysis (PC-DFA) of these samples produced clear separate clustering of isolates, based on the ST. Analysis of data using partial least squares-discriminant analysis (PLS-DA), incorporating cross-validation, indicated a high prediction accuracy of 91.19% for ST131. These results suggest that FT-IR spectroscopy could be a useful method for the rapid identification of members of important UPEC STs.
authors.affiliation: Microbiology and Virology Unit, School of Medicine, University of Manchester, Manchester, UK.
authors.forename: S E
authors.initials: SE
authors.lastname: Dawson
authors.forename: T
authors.initials: T
authors.lastname: Gibreel
authors.forename: N
authors.initials: N
authors.lastname: Nicolaou
authors.forename: H
authors.initials: H
authors.lastname: AlRabiah
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.forename: R
authors.initials: R
authors.lastname: Goodacre
authors.forename: M
authors.initials: M
authors.lastname: Upton
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D006801:Humans; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D056689:Uropathogenic Escherichia coli / Q000737:chemistry / Q000145:classification*