title: Detection of a soluble form of CD109 in serum of CD109 transgenic and tumor xenografted mice.
abstract: CD109, a glycosylphosphatidylinositol-anchored glycoprotein, is expressed at high levels in some human tumors including squamous cell carcinomas. As CD109 is reportedly cleaved by furin and its soluble form is secreted into culture medium in vitro, we hypothesized that CD109 could serve as a tumor marker in vivo. In this study, we investigated CD109 as a novel serum tumor marker using transgenic mice that overexpress mouse CD109 (mCD109-TG mice) and tumor xenografted mice inoculated with human CD109 (hCD109)-overexpressing HEK293 cells. In sera and urine of mCD109-TG mice, mCD109 was detected using western blotting. In xenografted mice, hCD109 secreted from inoculated tumors was detected in sera, using western blotting and CD109 ELISA. Concentrations of tumor-secreted CD109 increased proportionally as tumors enlarged. Concentrations of secreted CD109 decreased notably by 17 h after tumor resection, and became undetectable 48 h after resection. The half-life of tumor-secreted CD109 was about 5.86±0.17 h. These results indicate that CD109 is present in serum as a soluble form, and suggest its potential as a novel tumor marker in patients with cancers that express CD109.
authors.affiliation: Department of Pathology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan ; Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sakakura
authors.affiliation: Department of Pathology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan ; Department of Pathology, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Murakumo
authors.affiliation: Department of Pathology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Mii
authors.affiliation: Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
authors.forename: Sumitaka
authors.initials: S
authors.lastname: Hagiwara
authors.affiliation: Department of Pathology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Kato
authors.affiliation: Department of Pathology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Asai
authors.affiliation: Department of Pathology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Hoshino
authors.affiliation: Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Yamamoto
authors.affiliation: Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, Kasugai, Aichi, Japan.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Sobue
authors.affiliation: Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, Kasugai, Aichi, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Ichihara
authors.affiliation: Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Ueda
authors.affiliation: Department of Pathology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
authors.forename: Masahide
authors.initials: M
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000097:blood* / Q000235:genetics / Q000652:urine; D004195:Disease Models, Animal; D005260:Female; D057809:HEK293 Cells; D064593:Heterografts; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D009363:Neoplasm Proteins / Q000097:blood* / Q000235:genetics / Q000652:urine; D009369:Neoplasms / Q000097:blood* / Q000235:genetics / Q000473:pathology; D047368:Tumor Burden
chemical_list: D015703:Antigens, CD; C548464:CD109 protein, mouse; D009363:Neoplasm Proteins