title: Trafficking phenotype and production of granzyme B by double negative B cells (IgG(+)IgD(-)CD27(-)) in the elderly.
abstract: The impairment of humoral immune response in elderly humans has been extensively demonstrated. We have reported the increase of memory B cells (IgG(+)IgD(-)CD27(-), double negative, DN) population in the elderly, in which there is also a typical inflammatory micro-environment. In order to evaluate whether this pro-inflammatory status could influence the trafficking phenotype of naïve/memory B cells, we have assessed the expression of CCR7, CCR6, CXCR3, CXCR4, CXCR5 and CD62L on naïve/memory B cell subpopulations in young and elderly subjects. Moreover, the combination of pro-inflammatory interleukin-21 (IL-21) and B cell receptor (BCR) stimulation enables B cells to produce and secrete granzyme B (GrB), which plays a critical role in early anti-viral immune responses, in the regulation of autoimmune mechanisms and in cancer immunosurveillance. Our data demonstrate that in the elderly, naïve/memory B cell populations present a different expression of the studied receptors that could be discussed in terms of "inflamm-aging". In particular IgG(+)IgD(-)CD27(-) DN B cells show a tissue trafficking phenotype and they can be stimulated to produce GrB.
authors.affiliation: Immunosenescence Unit, Department of Pathobiology and Medical and Forensic Biotechnologies, University of Palermo, Corso Tukory 211, 90134 Palermo, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Bulati
authors.affiliation: Immunosenescence Unit, Department of Pathobiology and Medical and Forensic Biotechnologies, University of Palermo, Corso Tukory 211, 90134 Palermo, Italy.
authors.forename: Silvio
authors.initials: S
authors.lastname: Buffa
authors.affiliation: Immunosenescence Unit, Department of Pathobiology and Medical and Forensic Biotechnologies, University of Palermo, Corso Tukory 211, 90134 Palermo, Italy.
authors.forename: Adriana
authors.initials: A
authors.lastname: Martorana
authors.affiliation: Immunosenescence Unit, Department of Pathobiology and Medical and Forensic Biotechnologies, University of Palermo, Corso Tukory 211, 90134 Palermo, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Candore
authors.affiliation: Immunosenescence Unit, Department of Pathobiology and Medical and Forensic Biotechnologies, University of Palermo, Corso Tukory 211, 90134 Palermo, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Lio
authors.affiliation: Immunosenescence Unit, Department of Pathobiology and Medical and Forensic Biotechnologies, University of Palermo, Corso Tukory 211, 90134 Palermo, Italy.
authors.forename: Calogero
authors.initials: C
authors.lastname: Caruso
authors.affiliation: Immunosenescence Unit, Department of Pathobiology and Medical and Forensic Biotechnologies, University of Palermo, Corso Tukory 211, 90134 Palermo, Italy. Electronic address: giuseppina.colonnaromano@unipa.it.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Colonna-Romano
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000276:immunology*; D016175:B-Lymphocyte Subsets / Q000201:enzymology*; D053804:Granzymes / Q000096:biosynthesis*; D006801:Humans; D007072:Immunoglobulin D / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007378:Interleukins / Q000502:physiology; D019041:L-Selectin / Q000378:metabolism; D010641:Phenotype; D054387:Receptors, CXCR / Q000378:metabolism; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000378:metabolism; D000098546:Interleukin-21
chemical_list: D007072:Immunoglobulin D; D007074:Immunoglobulin G; D007378:Interleukins; D054387:Receptors, CXCR; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D019041:L-Selectin; D053804:Granzymes; D000098546:Interleukin-21
keywords: B lymphocytes; Chemokine receptors; Elderly; Granzyme B; IL-21; Inflamm-aging