title: Epigallocatechin-3-gallate (EGCG) attenuates concanavalin A-induced hepatic injury in mice.
abstract: (-)-Epigallocatechin-3-gallate (EGCG) is the most abundant polyphenolic compound present in green tea and has been shown to possess anti-inflammatory and anti-oxidative properties. In this study, we investigated the protective effects of EGCG against concanavalin A (ConA)-induced liver injury and the underlying mechanisms. EGCG (5 mg/kg) was administered orally by gavage to mice twice daily for 10 days before an intravenous injection of ConA. We found that EGCG effectively rescued lethality, improved hepatic pathological damage, and decreased serum levels of alanine aminotransaminase (ALT) in ConA-challenged mice. Furthermore, EGCG also significantly prevented the release of tumor necrosis factor (TNF)-α, interferon (IFN)-γ, interleukin (IL)-4, and IL-6 in serum, reduced malondialdehyde (MDA) levels, and restored glutathione (GSH) content and superoxide dismutase (SOD) activity in liver tissues from ConA-challenged mice. Finally, nuclear factor (NF)-κB activation and expression levels of Toll-like receptor (TLR) 2, TLR4 and TLR9 protein in liver tissues were significantly inhibited by EGCG pretreatment. Taken together, our data suggest that EGCG possesses hepatoprotective properties against ConA-induced liver injury through its anti-inflammatory and anti-oxidant actions.
authors.affiliation: Department of Rheumatology and Immunology, Shengjing Hospital, China Medical University, Shenyang 110004, People's Republic of China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Rheumatology and Immunology, Shengjing Hospital, China Medical University, Shenyang 110004, People's Republic of China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Rheumatology and Immunology, Shengjing Hospital, China Medical University, Shenyang 110004, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Rheumatology and Immunology, Shengjing Hospital, China Medical University, Shenyang 110004, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Gastroenterology, Shengjing Hospital, China Medical University, Shenyang 110004, People's Republic of China. Electronic address: changqing.zheng@outlook.com.
authors.forename: Changqing
authors.initials: C
authors.lastname: Zheng
mesh_terms: D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology*; D003208:Concanavalin A / Q000633:toxicity*; D004195:Disease Models, Animal; D008297:Male; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000502:physiology; D051193:Toll-Like Receptors / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D051193:Toll-Like Receptors; D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; D002392:Catechin; C045651:epigallocatechin gallate
keywords: Concanavalin A; Hepatitis; Inflammation; Mice; Nuclear factor-κB; Oxidative stress; Toll-like receptors