title: Epidermal growth factor receptor localized to exosome membranes as a possible biomarker for lung cancer diagnosis.
abstract: Detection of drug-target proteins and biomarkers that are expressed in cancer tissue has significant potential for both diagnosis and treatment of cancer. However, current immuno-histochemical and cytogenetic analyses of biopsy specimens for pre-operational diagnosis are highly invasive and often difficult to apply to lung cancer patients. The purpose of this study was to evaluate the possible utility of determining epidermal growth factor receptor (EGFR) expression on exosomal membranes using a targeted ELISA with an anti-CD81 antibody as a capture antibody for lung cancer diagnosis. While soluble EGFR (sEGFR) levels in plasma were not remarkably different between lung cancer patients and normal controls, significantly higher exosomal EGFR expression levels were observed in 5/9 cancer cases compared to normal controls. These results suggest that measurement of exosomal protein levels could be useful for in vitro diagnosis, and that exosomal EGFR is a possible biomarker for characterization of lung cancer.
authors.affiliation: Laboratory of Biopharmaceutical Research, National Institute of Biomedical Innovation, Osaka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamashita
authors.affiliation: Laboratory of Biopharmaceutical Research, National Institute of Biomedical Innovation, Osaka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kamada
authors.affiliation: Laboratory of Biopharmaceutical Research, National Institute of Biomedical Innovation, Osaka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kanasaki
authors.affiliation: Laboratory of Biopharmaceutical Research, National Institute of Biomedical Innovation, Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Maeda
authors.affiliation: Laboratory of Biopharmaceutical Research, National Institute of Biomedical Innovation, Osaka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nagano
authors.affiliation: Laboratory of Biopharmaceutical Research, National Institute of Biomedical Innovation, Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Abe
authors.affiliation: Laboratory of Biopharmaceutical Research, National Institute of Biomedical Innovation, Osaka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Inoue
authors.affiliation: Laboratory of Biopharmaceutical Research, National Institute of Biomedical Innovation, Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshioka
authors.affiliation: Laboratory of Biopharmaceutical Research, National Institute of Biomedical Innovation, Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tsutsumi
authors.affiliation: Laboratory of Biopharmaceutical Research, National Institute of Biomedical Innovation, Osaka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Katayama
authors.affiliation: Department of Biochemistry, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Inoue
authors.affiliation: Laboratory of Biopharmaceutical Research, National Institute of Biomedical Innovation, Osaka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Tsunoda
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D017077:Culture Media, Conditioned; D004797:Enzyme-Linked Immunosorbent Assay; D066246:ErbB Receptors / Q000378:metabolism*; D055354:Exosomes / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000175:diagnosis*; D008297:Male; D008566:Membranes / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D008875:Middle Aged; D010957:Plasmids; D060225:Tetraspanin 28 / Q000378:metabolism
chemical_list: D015415:Biomarkers; D017077:Culture Media, Conditioned; D060225:Tetraspanin 28; D066246:ErbB Receptors