title: Influence of hydrophilic amino acids and GC-content on expression of recombinant proteins used in vaccines against foot-and-mouth disease virus in Escherichia coli.
abstract: Epitope-based protein expression in Escherichia coli can be improved by adjusting its amino acid composition and encoding genes. To that end, we analyzed 24 recombinant epitope proteins (rEPs) that carry multiple epitopes derived from VP1 protein of foot-and-mouth disease virus. High level expression of the rEPs was attributed to a high content of Arg, Asn, Asp and Thr, a low content of Gln, Pro and Lys, a high content of hydrophilic amino acids and a higher isoelectric point value resulting from abundant Arg. It is also attributed to the appropriate guanine and cytosine content in the encoding genes. The data provide a reference for adjusting the amino acid composition in designing epitope-based proteins used in vaccines and for adjusting the synonymous codons to improve their expressions in E. coli.
authors.affiliation: Department of Molecular Biology, Norman Bethune College of Medicine, Jilin University, Changchun, 130021, China.
authors.forename: Hongfei
authors.initials: H
authors.lastname: Wei
authors.forename: Mingli
authors.initials: M
authors.lastname: Fang
authors.forename: Min
authors.initials: M
authors.lastname: Wan
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Peiyin
authors.initials: P
authors.lastname: Zhang
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Hu
authors.forename: Xiuli
authors.initials: X
authors.lastname: Wu
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.forename: Chengfeng
authors.initials: C
authors.lastname: Jiao
authors.forename: Li
authors.initials: L
authors.lastname: Hua
authors.forename: Wenzhen
authors.initials: W
authors.lastname: Diao
authors.forename: Yue
authors.initials: Y
authors.lastname: Xiao
authors.forename: Yongli
authors.initials: Y
authors.lastname: Yu
authors.forename: Liying
authors.initials: L
authors.lastname: Wang
mesh_terms: D000596:Amino Acids / Q000235:genetics*; D001482:Base Composition; D004926:Escherichia coli / Q000235:genetics*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology*; D015870:Gene Expression; D007526:Isoelectric Point; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000235:genetics; D014765:Viral Vaccines / Q000737:chemistry / Q000235:genetics*
chemical_list: D000596:Amino Acids; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines