title: Comparison between concurrent and sequential chemoradiation for non-small cell lung cancerin vitro.
abstract: Current practice guidelines recommend the combination of chemotherapy and thoracic radiation for locally advanced non-small cell lung cancer (NSCLC). Previous meta-analyses have shown that concurrent chemoradiation (CCRT) may be superior to sequential chemoradiation (SCRT). However, few previousin vitrostudies have analyzed these two treatment schedules. In the current study, four lung cancer cell lines harboring wild-type epidermal growth factor receptor, comprising two squamous and two non-squamous cell lines, were used. The IC10concentrations of three platinum-based regimens were combined with radiation treatment. Cells were irradiated at 0, 2, 4, 6 and 8 Gy using a137Cs irradiator concurrently or sequentially. Surviving fractions (SFs) were plotted as a function of the radiation dose. In A549 cells, only the docetaxel (Doc) and carboplatin (Carbo) combination showed a significant radiosensitizing effect with CCRT treatment. For the other three cell lines, no difference was identified in the SFs between CCRT and SCRT. Anin vitromethod of comparing CCRT with SCRT was established using lung cancer cell lines. Overall, no significant difference was detected in the radiosensitizing effect of the two treatment schedules, with the exception of the A549 cell lines treated with Doc/Carbo.
authors.affiliation: Department of Internal Medicine, Kangwon National University Hospital, Chuncheon-si, Gangwon-do 200-722, Republic of Korea.
authors.forename: Seo-Young
authors.initials: SY
authors.lastname: Song
authors.affiliation: Hamon Cancer Center for Therapeutic Oncology and Simmons Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
authors.forename: Amit K
authors.initials: AK
authors.lastname: DAS
authors.affiliation: Hamon Cancer Center for Therapeutic Oncology and Simmons Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
authors.forename: John D
authors.initials: JD
authors.lastname: Minna
keywords: carcinoma; cell line; chemotherapy; concurrent; non-small cell lung cancer; radiation; sequential