title: No detection of xenotropic murine leukemia virus-related viruses in prostate cancer in Sanandaj, west of Iran.
abstract: BACKGROUND
  Multiple etiologies have been hypothesized for prostate cancer, including genetic defects and infectious agents. A recently reported gamaretrovirus, xenotropic murine leukemia virus-related virus (XMRV) has been reported to be detected in prostate cancer. However, this virus has not been detected in similar groups of patients in other studies. Herein, we sought to detect XMRV in prostate cancers and benign controls in Sanandaj, west of Iran.
  
  
  METHODS
  In a case-control study, genomic DNA was extracted from formalin fixed and paraffin embedded prostate tissues from a total of 163 Iranian patients. We developed a conventional and a nested PCR assay using primers targeting to an env specific sequence of XMRV. PCR assays were carried out on 63 prostate cancers and 100 benign prostate hyperplasias.
  
  
  RESULTS
  Beta-actin sequences were successfully detected in the DNA extracts from all prostate tissues, confirming DNA extraction integrity. We did not detect XMRV in samples either from prostate cancers or benign prostate hyperplasias using XMRV specific primers.
  
  
  CONCLUSIONS
  We conclude that in our population XMRV does not play a role in genesis of prostate cancer.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran E-mail : mazaher-kh@muk.ac.ir.
authors.forename: Mazaher
authors.initials: M
authors.lastname: Khodabandehloo
authors.forename: Weria
authors.initials: W
authors.lastname: Hosseini
authors.forename: Mohammad-Reza
authors.initials: MR
authors.lastname: Rahmani
authors.forename: Mohammad-Ali
authors.initials: MA
authors.lastname: Rezaee
authors.forename: Mohammad-Saied
authors.initials: MS
authors.lastname: Hakhamaneshi
authors.forename: Bahram
authors.initials: B
authors.lastname: Nikkhoo
authors.forename: Ali
authors.initials: A
authors.lastname: Jalili
mesh_terms: D000230:Adenocarcinoma / Q000209:etiology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004279:DNA, Viral / Q000235:genetics; D005500:Follow-Up Studies; D006801:Humans; D007492:Iran; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D016133:Polymerase Chain Reaction; D011379:Prognosis; D011467:Prostate / Q000821:virology; D011471:Prostatic Neoplasms / Q000209:etiology*; D012192:Retroviridae Infections / Q000150:complications* / Q000821:virology; D058008:Xenotropic murine leukemia virus-related virus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*
chemical_list: D004279:DNA, Viral