title: Hematological and histopathological changes in silver catfish Rhamdia quelen (Siluriformes) exposed to clomazone herbicide in the Madre River, Santa Catarina State, Southern Brazil.
abstract: This study evaluated the influence of the clomazone herbicide (2-[(2-chlorophenyl)methyl]-4,4-dimethyl-3-isoxazolidinone) contamination on the hematological parameters and histological changes in gills and liver of silver catfish (Rhamdia quelen) from Madre River, Santa Catarina State, Southern Brazil. Fish were collected between March 2010 and January 2012 at two different sites of the Madre River, one site receiving residual water (contaminated site) from rice culture (n=49) and another that do not receive residual water (reference site) (n=48). The herbicide clomazone analysis detected 3.40±1.70 μg/L in the contaminated site and 1.1±0.33 μg/L in the reference site. Fish from contaminated site showed increased (P<0.05) number of monocytes suggesting the possible defense response as a result of chronic exposure to clomazone. On the other hand, no difference was found in the hematocrit percentage, red blood cell count, total thrombocyte number, white blood cell count, lymphocytes, and neutrophils number. Fish from both sites showed histopathological changes in gills and liver, possibly caused by chronic exposure to contamination. The influence of herbicide sub doses on fish health is also discussed.
authors.affiliation: a AQUOS - Aquatic Organisms Health Laboratory, Aquaculture Department , Federal University of Santa Catarina (UFSC) , Florianópolis , Santa Catarina , Brazil.
authors.forename: Aline
authors.initials: A
authors.lastname: Brum
authors.forename: Geovana
authors.initials: G
authors.lastname: Dotta
authors.forename: Katina
authors.initials: K
authors.lastname: Roumbedakis
authors.forename: Eduardo L T
authors.initials: EL
authors.lastname: Gonçalves
authors.forename: Laura P
authors.initials: LP
authors.lastname: Garcia
authors.forename: Patrícia
authors.initials: P
authors.lastname: Garcia
authors.forename: Vildes M
authors.initials: VM
authors.lastname: Scussel
authors.forename: Maurício L
authors.initials: ML
authors.lastname: Martins
mesh_terms: D000818:Animals; D001938:Brazil; D002397:Catfishes / Q000097:blood* / Q000378:metabolism; D004781:Environmental Exposure; D005880:Gills / Q000378:metabolism / Q000473:pathology*; D006405:Hematology; D006540:Herbicides / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D006653:Histology; D007555:Isoxazoles / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D023303:Oxazolidinones / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D045483:Rivers / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity
chemical_list: D006540:Herbicides; D007555:Isoxazoles; D023303:Oxazolidinones; D014874:Water Pollutants, Chemical; C095255:clomazone