title: Long noncoding RNAs expression profile of the developing mouse heart.
abstract: Long noncoding RNAs (lncRNAs) represent a sub-group of noncoding RNAs that are longer than 200 nucleotides. The characterization of lncRNAs and their acceptance as crucial regulators of numerous developmental and biological pathways have suggested that the lncRNA study has gradually become one of the hot topics in the field of RNA biology. Many lncRNAs show spatially and temporally restricted expression patterns during embryogenesis and organogenesis. This study aimed to characterize the lncRNA profile of the fetal mouse heart at three key time points (embryonic day E11.5, E14.5, and E18.5) in its development, by performing a microarray lncRNAs screen. Gene Ontology analysis and ingenuity pathway analysis showed some significant gene functions and pathways were altered in heart development process. We compared lncRNAs profile between the three points (E14.5 vs. E11.5 [early development]; E18.5 vs. E14.5 [later development]). A total of 1,237 lncRNAs were found to have consistent fold changes (>2.0) between the three time points. Among them, 20 dysregulated lncRNAs were randomly selected and confirmed by real-time qRT-PCR. Additionally, bioinformatics analysis of AK011347 suggested it may be involved in heart development through the target gene Map3k7. In summary, this study identified differentially expressed lncRNAs in the three time points studied, and these lncRNAs may provide a new clue of mechanism of normal heart development.
authors.affiliation: Department of Pediatrics, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, 210029, People's Republic of China.
authors.forename: Jin Gai
authors.initials: JG
authors.lastname: Zhu
authors.forename: Ya Hui
authors.initials: YH
authors.lastname: Shen
authors.forename: Hai Lang
authors.initials: HL
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.forename: Ya Qing
authors.initials: YQ
authors.lastname: Shen
authors.forename: Xiang Qing
authors.initials: XQ
authors.lastname: Kong
authors.forename: Gui Xian
authors.initials: GX
authors.lastname: Song
authors.forename: Ling Mei
authors.initials: LM
authors.lastname: Qian
mesh_terms: D000818:Animals; D047108:Embryonic Development / Q000235:genetics*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000254:growth & development*; D051379:Mice; D038081:Organogenesis; D062085:RNA, Long Noncoding / Q000096:biosynthesis*; D015398:Signal Transduction
chemical_list: D062085:RNA, Long Noncoding
keywords: BIOINFORMATICS; HEART DEVELOPMENT; LONG NONCODING RNA; MICROARRAY