title: Whole body microwave irradiation for improved dacarbazine therapeutical action in cutaneous melanoma mouse model.
abstract: A cutaneous melanoma mouse model was used to test the efficacy of a new therapeutical approach that uses low doses of cytostatics in conjunction with mild whole body microwave exposure of 2.45 GHz in order to enhance cytostatics antitumoral effect. Materials and Methods. A microwave exposure system for C57BL/6 mouse whole body microwave irradiation was designed; groups of 40 mice (males and females) bearing experimental tumours were subjected to a combined therapy comprising low doses of dacarbazine in combination with mild whole body irradiation. Clinical parameters and serum cytokine testing using xMAP technology were performed. Results. The group that was subjected to combined therapy, microwave and cytostatic, had the best clinical evolution in terms of overall survival, tumour volume, and metastatic potential. At day 14 the untreated group had 100% mortality, while in the combined therapy group 40% of mice were surviving. Quantifying serum IL-1 β , IL-6, IL-10, IL-12 (p70), IFN- γ , GM-CSF, TNF- α , MIP-1 α , MCP-1, and KC during tumorigenesis and therapy found that the combined experimental therapy decreases all the inflammatory cytokines, except chemokine MCP-1 that was found increased, suggesting an increase of the anti-tumoral immune response triggered by the combined therapy. The overall metastatic process is decreased in the combined therapy group.
authors.affiliation: Immunology Department, Immunobiology Laboratory, "Victor Babes" National Institute of Pathology, 99-101 Splaiul Independentei, sector 5, Bucharest 050096, Romania.
authors.forename: Monica
authors.initials: M
authors.lastname: Neagu
authors.affiliation: Immunology Department, Immunobiology Laboratory, "Victor Babes" National Institute of Pathology, 99-101 Splaiul Independentei, sector 5, Bucharest 050096, Romania.
authors.forename: Carolina
authors.initials: C
authors.lastname: Constantin
authors.affiliation: National Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor Street, Magurele 077125, Romania.
authors.forename: Diana
authors.initials: D
authors.lastname: Martin
authors.affiliation: Department of Infectious Diseases and Immunology, Virology Division, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands.
authors.forename: Lucian
authors.initials: L
authors.lastname: Albulescu
authors.affiliation: National Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor Street, Magurele 077125, Romania.
authors.forename: Nicusor
authors.initials: N
authors.lastname: Iacob
authors.affiliation: National Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor Street, Magurele 077125, Romania.
authors.forename: Daniel
authors.initials: D
authors.lastname: Ighigeanu