title: Toxicity assessments of chalcone and some synthetic chalcone analogues in a zebrafish model.
abstract: The aim of this study was to investigate the in vivo toxicities of some novel synthetic chalcones. Chalcone and four chalcone analogues 1a-d were evaluated using zebrafish embryos following antibody staining to visualize their morphological changes and muscle fiber alignment. Results showed that embryos treated with 3'-hydroxychalcone (compound 1b) displayed a high percentage of muscle defects (96.6%), especially myofibril misalignment. Ultrastructural analysis revealed that compound 1b-treated embryos displayed many muscle defect phenotypes, including breakage and collapse of myofibrils, reduced cell numbers, and disorganized thick (myosin) and thin (actin) filaments. Taken together, our results provide in vivo evidence of the myotoxic effects of the synthesized chalcone analogues on developing zebrafish embryos.
authors.affiliation: Department of Chemistry, Tamkang University, 151, Yingzhuan Road, Danshui Dist., New Taipei City 25137, Taiwan. joanna20520@gmail.com.
authors.forename: Ya-Ting
authors.initials: YT
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Tamkang University, 151, Yingzhuan Road, Danshui Dist., New Taipei City 25137, Taiwan. thfong@tmu.edu.tw.
authors.forename: Tsorng-Harn
authors.initials: TH
authors.lastname: Fong
authors.affiliation: Department of Chemistry, Tamkang University, 151, Yingzhuan Road, Danshui Dist., New Taipei City 25137, Taiwan. chm7805@tmu.edu.tw.
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Tamkang University, 151, Yingzhuan Road, Danshui Dist., New Taipei City 25137, Taiwan. frank00634@hotmail.com.
authors.forename: Chao-Yuan
authors.initials: CY
authors.lastname: Chang
authors.affiliation: Department of Chemistry, Tamkang University, 151, Yingzhuan Road, Danshui Dist., New Taipei City 25137, Taiwan. 129180@mail.tku.edu.tw.
authors.forename: Yun-Hsin
authors.initials: YH
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Tamkang University, 151, Yingzhuan Road, Danshui Dist., New Taipei City 25137, Taiwan. cychern@mail.ncyu.edu.tw.
authors.forename: Ching-Yuh
authors.initials: CY
authors.lastname: Chern
authors.affiliation: Department of Chemistry, Tamkang University, 151, Yingzhuan Road, Danshui Dist., New Taipei City 25137, Taiwan. yauhung@mail.tku.edu.tw.
authors.forename: Yau-Hung
authors.initials: YH
authors.lastname: Chen
mesh_terms: D000014:Abnormalities, Drug-Induced / Q000473:pathology*; D000818:Animals; D002599:Chalcone / Q000031:analogs & derivatives* / Q000633:toxicity*; D004625:Embryo, Nonmammalian / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects; D018485:Muscle Fibers, Skeletal / Q000187:drug effects / Q000473:pathology*; D018482:Muscle, Skeletal / Q000002:abnormalities; D013723:Teratogens / Q000633:toxicity*; D015027:Zebrafish
chemical_list: D013723:Teratogens; D002599:Chalcone