title: Proteomic analyses of human cytomegalovirus strain AD169 derivatives reveal highly conserved patterns of viral and cellular proteins in infected fibroblasts.
abstract: Human cytomegalovirus (HCMV) particle morphogenesis in infected cells is an orchestrated process that eventually results in the release of enveloped virions. Proteomic analysis has been employed to reveal the complexity in the protein composition of these extracellular particles. Only limited information is however available regarding the proteome of infected cells preceding the release of HCMV virions. We used quantitative mass spectrometry to address the pattern of viral and cellular proteins in cells, infected with derivatives of the AD169 laboratory strain. Our analyses revealed a remarkable conservation in the patterns of viral and of abundant cellular proteins in cells, infected for 2 hours, 2 days, or 4 days. Most viral proteins increased in abundance as the infection progressed over time. Of the proteins that were reliably detectable by mass spectrometry, only IE1 (pUL123), pTRS1, and pIRS1 were downregulated at 4 days after infection. In addition, little variation of viral proteins in the virions of the different viruses was detectable, independent of the expression of the major tegument protein pp65. Taken together these data suggest that there is little variation in the expression program of viral and cellular proteins in cells infected with related HCMVs, resulting in a conserved pattern of viral proteins ultimately associated with extracellular virions.
authors.affiliation: Institute for Virology, University Medical Center of the Johannes Gutenberg-University Mainz, Obere Zahlbacher Str. 67, D-55131 Mainz, Germany. reyda@uni-mainz.de.
authors.forename: Sabine
authors.initials: S
authors.lastname: Reyda
authors.affiliation: Institute for Virology, University Medical Center of the Johannes Gutenberg-University Mainz, Obere Zahlbacher Str. 67, D-55131 Mainz, Germany. bueschni@uni-manz.de.
authors.forename: Nicole
authors.initials: N
authors.lastname: Büscher
authors.affiliation: Institute for Virology, University Medical Center of the Johannes Gutenberg-University Mainz, Obere Zahlbacher Str. 67, D-55131 Mainz, Germany. tenzer@uni-mainz.de.
authors.forename: Stefan
authors.initials: S
authors.lastname: Tenzer
authors.affiliation: Institute for Virology, University Medical Center of the Johannes Gutenberg-University Mainz, Obere Zahlbacher Str. 67, D-55131 Mainz, Germany. plachter@uni-mainz.de.
authors.forename: Bodo
authors.initials: B
authors.lastname: Plachter
mesh_terms: D002460:Cell Line; D003587:Cytomegalovirus / Q000737:chemistry / Q000502:physiology*; D005347:Fibroblasts / Q000737:chemistry / Q000821:virology*; D054884:Host-Pathogen Interactions; D006801:Humans; D013058:Mass Spectrometry; D011506:Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D013997:Time Factors; D014779:Virus Replication
chemical_list: D011506:Proteins; D020543:Proteome