title: Functional assessment of sensory functions after photothrombotic stroke in the barrel field of mice.
abstract: Motor, sensory and cognitive deficits are common impairments observed in human stroke as well as in animal stroke models. Using a battery of behavioural tests we assessed sensorimotor deficits after photothrombotic stroke localized within or beyond cortical representation of mouse sensory vibrissae. We found restricted, modality specific behavioural consequences in the acute post-stroke period. Among incorporated tests, adhesive removal test, novelty exploration test and sensory labyrinth task were sensitive to the somatosensory cortical deficits. Injured animals explored new objects significantly longer, they also needed distinctly more time to contact and to remove the adhesive tape placed on whiskers contralateral to the infarct. Moreover, we observed that after stroke animals were unable to solve the sensory labyrinth depending only upon tactile sensation from whiskers with injured cortical representation. Spontaneous recovery could be observed within the first post-stroke week for adhesive tape removal and within 14 days for labyrinth performance. However, for the novel object exploration we did not observed the recovery for the period of 18 days after stroke. Moreover, new object exploration test performance differed between the somatosensory and visual cortical impairments. We suggest that those three tests might be valuable in assessing the usefulness of therapies designed to support brain repair after experimental stroke.
authors.affiliation: Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 3 Pasteur St., 02-093 Warsaw, Poland. Electronic address: m.liguz@nencki.gov.pl.
authors.forename: Monika
authors.initials: M
authors.lastname: Liguz-Lecznar
authors.affiliation: Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 3 Pasteur St., 02-093 Warsaw, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Zakrzewska
authors.affiliation: Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 3 Pasteur St., 02-093 Warsaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Daniszewska
authors.affiliation: Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 3 Pasteur St., 02-093 Warsaw, Poland; Warsaw School of Social Science and Humanities, 19 Chodakowska St., 03-815 Warsaw, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Kossut
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000209:etiology / Q000473:pathology; D004195:Disease Models, Animal; D007758:Ear, Inner / Q000503:physiopathology; D005106:Exploratory Behavior / Q000502:physiology; D005456:Fluorescent Dyes; D008027:Light / Q000009:adverse effects; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000502:physiology; D011597:Psychomotor Performance; D012395:Rose Bengal / Q000633:toxicity; D013003:Somatosensory Cortex / Q000503:physiopathology*; D020886:Somatosensory Disorders / Q000175:diagnosis* / Q000209:etiology*; D020521:Stroke / Q000150:complications* / Q000209:etiology / Q000473:pathology*; D013927:Thrombosis / Q000150:complications; D014738:Vibrissae / Q000294:innervation
chemical_list: D005456:Fluorescent Dyes; D012395:Rose Bengal
keywords: Barrel cortex; Behaviour; Mice; Photothrombotic stroke