title: Myor/ABF-1 mRNA [corrected] Expression Marks Follicular Helper T Cells but Is Dispensable for Tfh Cell Differentiation and Function In Vivo.
abstract: Follicular T helper cells (Tfh) are crucial for effective antibody responses and long term T cell-dependent humoral immunity. Although many studies are devoted to this novel T helper cell population, the molecular mechanisms governing Tfh cell differentiation have yet to be characterized. MyoR/ABF-1 is a basic helix-loop-helix transcription factor that plays a role in the differentiation of the skeletal muscle and Hodgkin lymphoma. Here we show that MyoR mRNA is progressively induced during the course of Tfh-like cell differentiation in vitro and is expressed in Tfh responding to Alum-precipitated antigens in vivo. This expression pattern suggests that MyoR could play a role in the differentiation and/or function of Tfh cells. We tested this hypothesis using MyoR-deficient mice and found this deficiency had no impact on Tfh differentiation. Hence, MyoR-deficient mice developed optimal T-dependent humoral responses to Alum-precipitated antigens. In conclusion, MyoR is a transcription factor selectively up-regulated in CD4 T cells during Tfh cell differentiation in vitro and upon response to alum-protein vaccines in vivo, but the functional significance of this up-regulation remains uncertain.
authors.affiliation: Laboratoire d'Immunobiologie, Université Libre de Bruxelles, Gosselies, Belgium.
authors.forename: Delphine
authors.initials: D
authors.lastname: Debuisson
authors.affiliation: Laboratoire d'Immunobiologie, Université Libre de Bruxelles, Gosselies, Belgium.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Mari
authors.affiliation: Laboratoire d'Immunobiologie, Université Libre de Bruxelles, Gosselies, Belgium.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Denanglaire
authors.affiliation: Laboratoire d'Immunobiologie, Université Libre de Bruxelles, Gosselies, Belgium.
authors.forename: Oberdan
authors.initials: O
authors.lastname: Leo
authors.affiliation: Laboratoire d'Immunobiologie, Université Libre de Bruxelles, Gosselies, Belgium.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Andris
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D002454:Cell Differentiation / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D015854:Up-Regulation / Q000502:physiology*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C117673:Msc protein, mouse