title: High throughput RNAi screening identifies ID1 as a synthetic sick/lethal gene interacting with the common TP53 mutation R175H.
abstract: The TP53 mutation (R175H) is one of the most common mutations in human cancer. It is a highly attractive strategy for cancer therapy to find the genes that lead the R175H-expressing cancer cells. The aim of this study was to identify the synthetic sick/lethal gene interacting with R175H. Using lentiviral bar-coded comprehensive shRNA library and a tetracycline-inducible R175H expressed in the SF126 human glioblastoma cell line (SF126-tet-R175H), we conducted high-throughput screening to identify the candidate genes that induce synthetic sickness/lethality in R175H-expressing cells. We identified 906 candidate gene suppressions that may lead to accelerated cell growth inhibition in the presence of R175H. Inhibitor of differentiation 1 (ID1) was one of the candidate genes, and its suppression by siRNA resulted in the acceleration of growth inhibition in cell lines both transiently and endogenously expressing R175H but not in TP53-null cell lines or other common p53 mutants (such as R273H). Flow cytometry analysis showed that ID1 suppression resulted in G1 arrest, and the arrest was accelerated by the expression of R175H. ID1 is a synthetic sick/lethal gene that interacts with R175H and is considered to be a novel molecular target for cancer therapy in R175H-expressing cells.
authors.affiliation: Department of Clinical Oncology, IDAC, Tohoku University, Sendai, Miyagi 980-8575, Japan.
authors.forename: Hiroo
authors.initials: H
authors.lastname: Imai
authors.affiliation: Department of Clinical Oncology, IDAC, Tohoku University, Sendai, Miyagi 980-8575, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Kato
authors.affiliation: Department of Clinical Oncology, IDAC, Tohoku University, Sendai, Miyagi 980-8575, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Sakamoto
authors.affiliation: Department of Clinical Oncology, IDAC, Tohoku University, Sendai, Miyagi 980-8575, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kakudo
authors.affiliation: Department of Clinical Oncology, IDAC, Tohoku University, Sendai, Miyagi 980-8575, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Shimodaira
authors.affiliation: Department of Clinical Oncology, IDAC, Tohoku University, Sendai, Miyagi 980-8575, Japan.
authors.forename: Chikashi
authors.initials: C
authors.lastname: Ishioka
mesh_terms: D049109:Cell Proliferation; D004843:Epistasis, Genetic; D059585:G1 Phase Cell Cycle Checkpoints; D055785:Gene Knockdown Techniques; D005804:Genes, Lethal; D045325:HCT116 Cells; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000235:genetics*; D020125:Mutation, Missense; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism
chemical_list: C497453:ID1 protein, human; D051798:Inhibitor of Differentiation Protein 1; D034741:RNA, Small Interfering; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53