title: Application of nanoring amino-functionalized magnetic polymer dispersive micro-solid-phase extraction and ultra fast liquid chromatography-tandem mass spectrometry in dicyandiamide residue analysis of powdered milk.
abstract: In this study, a rapid and accurate ultra-fast liquid chromatography-tandem quadrupole mass spectrometry (UFLC-MS/MS) method combined with dispersive micro-solid-phase extraction (d-µ-SPE) using a core-shell nanoring amino-functionalized magnetic polymer (CS-NR-MP) was established and validated to determine trace dicyandiamide (DCD) in powdered milk. The developed d-µ-SPE cleanup procedure can dramatically reduce the matrix in samples, and lead to a significant reduction in absolute matrix effects. Chromatographic separation was performed on an Acquity UPLC BEH Amide column by using water-acetonitrile (9:91, v/v) as the mobile phase within 2 min. DCD was quantitatively analyzed by using DCD-(15)N2(13)C2 as an internal standard. The results showed that the recoveries were between 99.8 and 105.6% with RSDs in the range of 0.5-4.9%. The target compound had good linearity in the range of 0.1-20.0 µg L(-1) with a correlation coefficient (r) of 0.9996. The limit of quantification (LOQ) was 0.06 µg kg(-1). This method can be used for the rapid and sensitive determination of ultratrace DCD residue in powdered milk samples.
authors.affiliation: Zhejiang Provincial Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China; Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Chen
authors.affiliation: Medical School, Ningbo University, Ningbo, Zhejiang 315211, China.
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Zhou
authors.affiliation: Zhejiang Provincial Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China; Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China.
authors.forename: Yong-Gang
authors.initials: YG
authors.lastname: Zhao
authors.affiliation: Zhejiang Provincial Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China; Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China.
authors.forename: Sheng-Dong
authors.initials: SD
authors.lastname: Pan
authors.affiliation: Zhejiang Provincial Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China; Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China. Electronic address: jmcjc@163.com.
authors.forename: Mi-Cong
authors.initials: MC
authors.lastname: Jin
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D006146:Guanidines / Q000032:analysis*; D057230:Limit of Detection; D008280:Magnetics; D008892:Milk / Q000737:chemistry*; D011108:Polymers; D011208:Powders; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D006146:Guanidines; D011108:Polymers; D011208:Powders; C004711:dicyandiamido
keywords: Core–shell nanoring amino-functionalized magnetic polymer (CS-NR-MP); Dicyandiamide (DCD); Dispersive micro-solid-phase extraction (d-µ-SPE); Matrix effect; Powdered milk; Ultra-fast liquid chromatography–tandem quadrupole mass spectrometry (UFLC–MS/MS)