title: Expression of the mammalian Xenotropic Polytropic Virus Receptor 1 (XPR1) in tobacco leaves leads to phosphate export.
abstract: Phosphate homeostasis in multicellular eukaryotes depends on both phosphate influx and efflux. The mammalian Xenotropic Polytropic Virus Receptor 1 (XPR1) shares homology to the Arabidopsis PHO1, a phosphate exporter expressed in roots. However, phosphate export activity of XPR1 has not yet been demonstrated in a heterologous system. Here, wedemonstrate that transient expression in tobacco leaves of XPR1-GFP leads to specific phosphate export. Like PHO1-GFP, XPR1-GFP is localized predominantly to the endomembrane system in tobacco cells. These results show that tobacco leaves are a good heterologous system to study the transport activity of members of the PHO1/XPR1 family.
authors.affiliation: Department of Plant Molecular Biology, University of Lausanne, 1015 Lausanne, Switzerland.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Wege
authors.affiliation: Department of Plant Molecular Biology, University of Lausanne, 1015 Lausanne, Switzerland. Electronic address: yves.poirier@unil.ch.
authors.forename: Yves
authors.initials: Y
authors.lastname: Poirier
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D006706:Homeostasis; D010710:Phosphates / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots; D030821:Plants, Genetically Modified; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis / Q000235:genetics*; D011991:Receptors, Virus / Q000096:biosynthesis / Q000235:genetics*; D014026:Nicotiana / Q000254:growth & development / Q000378:metabolism; D000089842:Xenotropic and Polytropic Retrovirus Receptor
chemical_list: D029681:Arabidopsis Proteins; D010710:Phosphates; D043562:Receptors, G-Protein-Coupled; D011991:Receptors, Virus; D000089842:Xenotropic and Polytropic Retrovirus Receptor
keywords: PHO1; Phosphate export; Tobacco; Transporter; XPR1