title: [Study on effective substances of tea for chemoprevention of lung cancer based on principal component analysis].
abstract: OBJECTIVE
  To study the effective substances of tea for chemoprevention of lung cancer based on Principal Component Analysis (PCA).
  
  
  METHODS
  UPLC was used to determine the content of each component in tea, MTT was used to analyze the survival of CSE-induced NHBE cells. DTNB and the colorimetric assay were used to detect the rate of GSH/GSSG, PCA was used to calculate the correlation coefficient matrix, eigen values and variance of contribution ratio to chemoprevention of lung cancer.
  
  
  RESULTS
  Longjing (L) was the most effective one to protect NHBE cells from damaging, and the IC50 of L for prevention of NHBE cells was 0.2559 mg/mL and could make the GSH/GSSG ratio recovery ranging from 0.142 to 0.858 in a dose-dependent manner. The contribution of GCG prevention of lung cancer was 79.602% and that of GA was 11.037% by PCA.
  
  
  CONCLUSIONS
  Different kinds of tea have different protective effect on NHBE cells. GCGC and GA are the main active ingredients in tea for chemoprevention of lung cancer by PCA.
authors.affiliation: Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Traditional Chinese Medicine, Nanjing 210028, China. hunanjinping@163.com
authors.forename: Ping
authors.initials: P
authors.lastname: Jin
authors.affiliation: Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Traditional Chinese Medicine, Nanjing 210028, China.
authors.forename: Fen-Xia
authors.initials: FX
authors.lastname: Zhu
authors.affiliation: Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Traditional Chinese Medicine, Nanjing 210028, China.
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Tan
authors.affiliation: Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Traditional Chinese Medicine, Nanjing 210028, China.
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Liu
authors.affiliation: Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Traditional Chinese Medicine, Nanjing 210028, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.affiliation: Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Traditional Chinese Medicine, Nanjing 210028, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Feng
authors.affiliation: Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Traditional Chinese Medicine, Nanjing 210028, China.
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Jia
mesh_terms: D016588:Anticarcinogenic Agents / Q000737:chemistry / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D001980:Bronchi / Q000166:cytology; D002392:Catechin / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005707:Gallic Acid / Q000494:pharmacology; D006801:Humans; D008175:Lung Neoplasms / Q000517:prevention & control*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D025341:Principal Component Analysis; D012906:Smoke / Q000009:adverse effects; D012907:Smoking / Q000009:adverse effects; D013662:Tea / Q000737:chemistry* / Q000145:classification
chemical_list: D016588:Anticarcinogenic Agents; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D012906:Smoke; D013662:Tea; D005707:Gallic Acid; D002392:Catechin