title: Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in an STI population: performances of the Presto CT-NG assay, the Lightmix Kit 480 HT CT/NG and the COBAS Amplicor with urine specimens and urethral/cervicovaginal samples.
abstract: OBJECTIVE
  This study assessed the performances of the Presto CT-NG assay, the Lightmix Kit 480 HT CT/NG and the COBAS Amplicor for Chlamydia trachomatis and Neisseria gonorrhoeae detection.
  
  
  METHODS
  A cross-sectional study design.
  
  
  METHODS
  Izore, Centre for Diagnosing Infectious Diseases in Friesland, the Netherlands, tested samples sent from regional sexually transmitted infection (STI) outpatient clinics and regional hospitals from the province Friesland, the Netherlands.
  
  
  METHODS
  Samples were collected from 292 men and 835 women. These samples included 560 urine samples and 567 urethral/cervicovaginal samples.
  
  
  METHODS
  The primary outcome measure is C trachomatis infection. No secondary outcome measures are available.
  
  
  RESULTS
  The sensitivity, specificity, positive predicative value (PPV) and negative predictive value (NPV) for C trachomatis detection in urine samples using the Presto CT-NG assay were 100%, 99.8%, 98.1% and 100%, respectively; for the Lightmix Kit 480 HT CT/NG: 94.2%, 99.8%, 96.1% and 99.4%, respectively; for the COBAS Amplicor: 92.3%, 99.6%, 96% and 99.2%, respectively. The sensitivity, specificity, PPV and NPV for C trachomatis detection in urethral/cervicovaginal swabs using the Presto CT-NG assay and the COBAS Amplicor were 100%, 99.8%, 97.7% and 100%, respectively; for the Lightmix Kit 480 HT CT/NG: 100%, 99.6%, 97.7% and 100%, respectively. Calculations for N gonorrhoeae could not be made due to a low prevalence.
  
  
  CONCLUSIONS
  All three assays had a high sensitivity, specificity, PPV and NPV for C trachomatis, with best performance for the Presto CT-NG assay.
authors.affiliation: Izore, Centre for Infectious Diseases Friesland, Leeuwarden, The Netherlands.
authors.forename: T A
authors.initials: TA
authors.lastname: Schuurs
authors.forename: S P
authors.initials: SP
authors.lastname: Verweij
authors.forename: J F L
authors.initials: JF
authors.lastname: Weel
authors.forename: S
authors.initials: S
authors.lastname: Ouburg
authors.forename: S A
authors.initials: SA
authors.lastname: Morré
keywords: Infectious Diseases; Microbiology