title: Synthesis of squaraine dyes under mild conditions: applications for labelling and sensing of biomolecules.
abstract: We report the synthesis of squaraine dyes under mild conditions by carbodiimide activation of squaric acid or semi-squaraine dyes. Despite low yields when the reaction was carried out in solution, these conditions were successfully applied to efficient peptide labelling on resin and nucleic acid sensing in solution.
authors.affiliation: Department of Bioengineering, Imperial College London, London, SW7 2AZ, UK. sladame@imperial.ac.uk.
authors.forename: Mazen Haj
authors.initials: MH
authors.lastname: Sleiman
authors.forename: Sylvain
authors.initials: S
authors.lastname: Ladame
mesh_terms: D015374:Biosensing Techniques; D002234:Carbodiimides / Q000737:chemistry; D003503:Cyclobutanes / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D015394:Molecular Structure; D010446:Peptide Fragments / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*
chemical_list: D002234:Carbodiimides; D003503:Cyclobutanes; D005456:Fluorescent Dyes; D010446:Peptide Fragments; D020135:Peptide Nucleic Acids; D010636:Phenols; C480596:squaraine; D004247:DNA