title: Genetic and phenotypic analyses of carpel development in Arabidopsis.
abstract: Carpels are the female reproductive organs of the flower, organized in a gynoecium, which is arguably the most complex organ of a plant. The gynoecium provides protection for the ovules, helps to discriminate between male gametophytes, and facilitates successful pollination. After fertilization, it develops into a fruit, a specialized organ for seed protection and dispersal. To carry out all these functions, coordinated patterning and tissue specification within the developing gynoecium have to be achieved. In this chapter, we describe different methods to characterize defects in carpel patterning and morphogenesis associated with developmental mutations as well as a list of reporter lines that can be used to facilitate genetic analyses.
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia, Valencia, Spain.
authors.forename: Vicente
authors.initials: V
authors.lastname: Balanzà
authors.forename: Patricia
authors.initials: P
authors.lastname: Ballester
authors.forename: Monica
authors.initials: M
authors.lastname: Colombo
authors.forename: Chloé
authors.initials: C
authors.lastname: Fourquin
authors.forename: Irene
authors.initials: I
authors.lastname: Martínez-Fernández
authors.forename: Cristina
authors.initials: C
authors.lastname: Ferrándiz
mesh_terms: D000814:Aniline Compounds / Q000378:metabolism; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*; D035264:Flowers / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D008031:Lignin / Q000378:metabolism; D010641:Phenotype; D010797:Phthalimides / Q000494:pharmacology; D011058:Pollen / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D013194:Staining and Labeling; D016707:Tissue Fixation
chemical_list: D000814:Aniline Compounds; D010797:Phthalimides; C002687:alpha-naphthylphthalamic acid; C017006:aniline blue; D008031:Lignin