title: Comparative proteomics profile of lipid-cumulating oleaginous yeast: an iTRAQ-coupled 2-D LC-MS/MS analysis.
abstract: Accumulation of intracellular lipid in oleaginous yeast cells has been studied for providing an alternative supply for energy, biofuel. Numerous studies have been conducted on increasing lipid content in oleaginous yeasts. However, few explore the mechanism of the high lipid accumulation ability of oleaginous yeast strains at the proteomics level. In this study, a time-course comparative proteomics analysis was introduced to compare the non-oleaginous yeast Saccharomyces cerevisiae, with two oleaginous yeast strains, Cryptococcus albidus and Rhodosporidium toruloides at different lipid accumulation stages. Two dimensional LC-MS/MS approach has been applied for protein profiling together with isobaric tag for relative and absolute quantitation (iTRAQ) labelling method. 132 proteins were identified when three yeast strains were all at early lipid accumulation stage; 122 and 116 proteins were found respectively within cells of three strains collected at middle and late lipid accumulation stages. Significantly up-regulation or down-regulation of proteins were experienced among comparison. Essential proteins correlated to lipid synthesis and regulation were detected. Our approach provides valuable indication and better understanding for lipid accumulation mechanism from proteomics level and would further contribute to genetic engineering of oleaginous yeasts.
authors.affiliation: School of Chemical and Biomedical Engineering, College of Engineering, Nanyang Technological University, Singapore.
authors.forename: Jiahua
authors.initials: J
authors.lastname: Shi
authors.affiliation: School of Chemical and Biomedical Engineering, College of Engineering, Nanyang Technological University, Singapore.
authors.forename: Huixing
authors.initials: H
authors.lastname: Feng
authors.affiliation: School of Chemical and Biomedical Engineering, College of Engineering, Nanyang Technological University, Singapore.
authors.forename: Jaslyn
authors.initials: J
authors.lastname: Lee
authors.affiliation: School of Chemical and Biomedical Engineering, College of Engineering, Nanyang Technological University, Singapore.
authors.forename: Wei
authors.initials: W
authors.lastname: Ning Chen
mesh_terms: D003454:Cryptococcus / Q000235:genetics / Q000378:metabolism*; D050356:Lipid Metabolism / Q000502:physiology*; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins