title: Multimodal SPION-CREKA peptide based agents for molecular imaging of microthrombus in a rat myocardial ischemia-reperfusion model.
abstract: Microthrombosis plays a key role in many cardiovascular diseases. Although it is not difficult to localize thrombus within large or middle-sized vessels, the noninvasive diagnostic regimen for the detection of microthrombus remains scarce. Here we developed a nanoagent by conjucting superparamagnetic iron-oxide nanoparticle with fluorophore and a targeting element, CREKA, a peptide with special affinity for fibrin. In a rat model of myocardial ischemia-reperfusion (MI/R), the multimodal nanoagents were readily and selectively accumulated within microthrombosis, which was detectable by both magnetic resonance and optical imaging modalities. The fibrin-targeted nanoagent could be expected to have utility not only in molecular imaging of fibrin, understanding the mechanisms of microcirculation disorders, but also in targeted therapy with fibrinolytic agents.
authors.affiliation: Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Song
authors.affiliation: Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, China.
authors.forename: Zheyong
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Xu
authors.affiliation: Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, China.
authors.forename: Daoyuan
authors.initials: D
authors.lastname: Ren
authors.affiliation: School of Pharmacy, Fudan University, Key Laboratory of Smart Drug Delivery, Ministry of Education, 826 Zhangheng Road, Shanghai 201203, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Radiology, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, China.
authors.forename: Xinde
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Cardiology, Shanghai East Hospital, Tongji University, 150 Jimo Road, Shanghai 200120, China.
authors.forename: Yunli
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Radiology, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Laboratory, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, China.
authors.forename: Hongxiang
authors.initials: H
authors.lastname: Gao
authors.affiliation: Biomedical Research Center, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, China.
authors.forename: Jiayun
authors.initials: J
authors.lastname: Hou
authors.affiliation: School of Pharmacy, Fudan University, Key Laboratory of Smart Drug Delivery, Ministry of Education, 826 Zhangheng Road, Shanghai 201203, China. Electronic address: zqpang@fudan.edu.cn.
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Pang
authors.affiliation: Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, China. Electronic address: juyingqian@126.com.
authors.forename: Juying
authors.initials: J
authors.lastname: Qian
authors.affiliation: Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, China; Institute of Biomedical Science, Fudan University, 180 Feng Lin Road, Shanghai 200032, China. Electronic address: ge.junbo2@zs-hospital.sh.cn.
authors.forename: Junbo
authors.initials: J
authors.lastname: Ge
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D001792:Blood Platelets / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D003911:Dextrans; D004195:Disease Models, Animal; D006801:Humans; D058185:Magnetite Nanoparticles; D008297:Male; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D064847:Multimodal Imaging / Q000379:methods*; D015428:Myocardial Reperfusion Injury / Q000150:complications / Q000175:diagnosis*; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D009842:Oligopeptides / Q000494:pharmacology; D061848:Optical Imaging; D010316:Particle Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D055672:Static Electricity; D013927:Thrombosis / Q000150:complications* / Q000175:diagnosis*
chemical_list: D003911:Dextrans; D058185:Magnetite Nanoparticles; D009842:Oligopeptides; C542438:cysteinyl-arginyl-glutamyl-lysyl-alanyl; C065507:ferumoxides
keywords: Fibrin; Magnetic resonance imaging; Microthrombosis; Multimodal nanoagents; Optical imaging