title: Variations in the GST activity are associated with single and combinations of GST genotypes in both male and female diabetic patients.
abstract: In the present cross sectional study, we aimed to ascertain the relative associations of GST genotypes with GST activity variations and also with the risk to DMT2 predisposition among men and women separately. Clinical samples obtained from 244 DMT2 cases (120 Males and 124 Females) and 228 controls (117 Males and 111 Females) belonging to Asian Indian ethnicity were used to test for glycemic index, lipid profile, GST activity and GST genotypes. The frequencies of single and combinations of GST genotypes were statistically examined for their association with DMT2 risk among both study groups. The GST activity is significantly lowered in DMT2 group compared to controls (p = < 0.001). This reduction is found to be subjective to single and combinations of GST genotypes among diabetic patients. The frequency distribution for single, double and triple combinations of genotypes of GSTT1, GSTM1 and GSTP1 showed the varying degrees of association with DMT2 risk from 0.5 to 5.6-fold among male and female patients (for all associations, p value was <0.05). Interestingly, GST activity was lowered in both male and female patients with single or combinational genotypes of GSTM1 (Null), GSTT1 (Null), and P1 (V/V) (for all associations, p value was = <0.0001). The reduced anti-oxidant capacity among diabetic patients with certain GST genotypes may have some important implications for disease diagnosis and therapy.
authors.affiliation: Department of Genetics, University College of Science, Osmania University, Hyderabad, Andhra Pradesh, India.
authors.forename: Durga Koteswara
authors.initials: DK
authors.lastname: Rao
authors.forename: Noor Ahmad
authors.initials: NA
authors.lastname: Shaik
authors.forename: Ahmad
authors.initials: A
authors.lastname: Imran
authors.forename: Dwarakanath K
authors.initials: DK
authors.lastname: Murthy
authors.forename: Eswar
authors.initials: E
authors.lastname: Ganti
authors.forename: Chitralekha
authors.initials: C
authors.lastname: Chinta
authors.forename: Hanmantha
authors.initials: H
authors.lastname: Rao
authors.forename: Nazia Sultana
authors.initials: NS
authors.lastname: Shaik
authors.forename: Jumana Yousuf
authors.initials: JY
authors.lastname: Al-Aama
mesh_terms: D000328:Adult; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000235:genetics; D011110:Polymorphism, Genetic; D012307:Risk Factors
chemical_list: D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; C413545:glutathione S-transferase T1