title: Water stratification affects the microeukaryotic community in a subtropical deep reservoir.
abstract: Producers, consumers, and decomposers are the three key functional groups that form the basis of all ecosystems. But, little is known about how these functional groups coexist with each other in aquatic environments, particularly in subtropical reservoirs. In this study, we describe the nature of microeukaryotic communities in a subtropical deep reservoir during the strongly stratified period. Denaturing gradient gel electrophoresis gel band sequencing, pyrosequencing, and light microscopy were used together to facilitate an in-depth investigation of the community structure of phytoplankton, zooplankton, and fungi. Our results showed that thermal and oxygen stratification shaped the composition of the phytoplankton, zooplankton, and fungi populations in the reservoir. Stratification was evident among ecological functional groups in autumn: producers and consumers were overwhelmingly dominant in the epilimnion characterized by high temperatures and oxygen levels, whereas decomposers were inclined to inhabit the hypolimnion. These results contribute to our understanding of the relationship of ecosystem functional groups in the man-made aquatic systems and have important practical implications for reservoir management. Results suggest that the strategies for the control of eutrophication and harmful algal bloom prevention should focus on a fuller understanding of the consequences of both thermal stratification and vertical distribution of microplankton.
authors.affiliation: Aquatic EcoHealth Group, Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Yu
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Yu
authors.forename: Lemian
authors.initials: L
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Lv
mesh_terms: D000818:Animals; D058448:Biota; D058645:Denaturing Gradient Gel Electrophoresis; D005658:Fungi / Q000145:classification* / Q000302:isolation & purification; D008853:Microscopy; D010100:Oxygen / Q000032:analysis; D010839:Phytoplankton / Q000145:classification* / Q000302:isolation & purification; D012621:Seasons; D017422:Sequence Analysis, DNA; D013696:Temperature; D014329:Tropical Climate; D014867:Water / Q000737:chemistry / Q000469:parasitology*; D014871:Water Microbiology; D015048:Zooplankton / Q000145:classification* / Q000302:isolation & purification
chemical_list: D014867:Water; D010100:Oxygen
keywords: 454 pyrosequencing; PCR-DGGE; fungi; phytoplankton; reservoir stratification; zooplankton