title: Protein levels and colony development of Africanized and European honey bees fed natural and artificial diets.
abstract: Pollen substitute diets are a valuable resource for maintaining strong and health honey bee colonies. Specific diets may be useful in one region or country and inadequate or economically unviable in others. We compared two artificial protein diets that had been formulated from locally-available ingredients in Brazil with bee bread and a non-protein sucrose diet. Groups of 100 newly-emerged, adult workers of Africanized honey bees in Brazil and European honey bees in the USA were confined in small cages and fed on one of four diets for seven days. The artificial diets included a high protein diet made of soy milk powder and albumin, and a lower protein level diet consisting of soy milk powder, brewer's yeast and rice bran. The initial protein levels in newly emerged bees were approximately 18-21 µg/µL hemolymph. After feeding on the diets for seven days, the protein levels in the hemolymph were similar among the protein diet groups (~37-49 µg/µL after seven days), although Africanized bees acquired higher protein levels, increasing 145 and 100% on diets D1 and D2, respectively, versus 83 and 60% in the European bees. All the protein diets resulted in significantly higher levels of protein than sucrose solution alone. In the field, the two pollen substitute diets were tested during periods of low pollen availability in the field in two regions of Brazil. Food consumption, population development, colony weight, and honey production were evaluated to determine the impact of the diets on colony strength parameters. The colonies fed artificial diets had a significant improvement in all parameters, while control colonies dwindled during the dearth period. We conclude that these two artificial protein diets have good potential as pollen substitutes during dearth periods and that Africanized bees more efficiently utilize artificial protein diets than do European honey bees.
authors.affiliation: Departamento de Biologia, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil michelle.manfrini@unifesp.br.
authors.forename: M M
authors.initials: MM
authors.lastname: Morais
authors.forename: A P
authors.initials: AP
authors.lastname: Turcatto
authors.forename: R A
authors.initials: RA
authors.lastname: Pereira
authors.forename: T M
authors.initials: TM
authors.lastname: Francoy
authors.forename: K R
authors.initials: KR
authors.lastname: Guidugli-Lazzarini
authors.forename: L S
authors.initials: LS
authors.lastname: Gonçalves
authors.forename: J M V
authors.initials: JM
authors.lastname: de Almeida
authors.forename: J D
authors.initials: JD
authors.lastname: Ellis
authors.forename: D
authors.initials: D
authors.lastname: De Jong
mesh_terms: D000818:Animals; D001516:Bees; D001938:Brazil; D004032:Diet / Q000662:veterinary*; D004044:Dietary Proteins / Q000008:administration & dosage*; D005502:Food; D006458:Hemolymph; D011058:Pollen; D011429:Propolis / Q000008:administration & dosage*; D045730:Soy Foods
chemical_list: D004044:Dietary Proteins; D011429:Propolis