title: Comparison of the molecular profiles of human embryonic and induced pluripotent stem cells of isogenic origin.
abstract: Many studies have compared the genetic and epigenetic profiles of human induced pluripotent stem cells (hiPSCs) to human embryonic stem cells (hESCs) and yet the picture remains unclear. To address this, we derived a population of neural precursor cells (NPCs) from the H1 (WA01) hESC line and generated isogenic iPSC lines by reprogramming. The gene expression and methylation profile of three lines were compared to the parental line and intermediate NPC population. We found no gene probe with expression that differed significantly between hESC and iPSC samples under undifferentiated or differentiated conditions. Analysis of the global methylation pattern also showed no significant difference between the two PSC populations. Both undifferentiated populations were distinctly different from the intermediate NPC population in both gene expression and methylation profiles. One point to note is that H1 is a male line and so extrapolation to female lines should be cautioned. However, these data confirm our previous findings that there are no significant differences between hESCs and hiPSCs at the gene expression or methylation level.
authors.affiliation: NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: mallonb@mail.nih.gov.
authors.forename: Barbara S
authors.initials: BS
authors.lastname: Mallon
authors.affiliation: NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Rebecca S
authors.initials: RS
authors.lastname: Hamilton
authors.affiliation: Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Olga A
authors.initials: OA
authors.lastname: Kozhich
authors.affiliation: Bioinformatics Section, Intramural IT and Bioinformatics Program, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Kory R
authors.initials: KR
authors.lastname: Johnson
authors.affiliation: Bioinformatics Section, Intramural IT and Bioinformatics Program, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Yang C
authors.initials: YC
authors.lastname: Fann
authors.affiliation: NIH Center for Regenerative Medicine, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Mahendra S
authors.initials: MS
authors.lastname: Rao
authors.affiliation: NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA; Craniofacial and Skeletal Disease Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Pamela G
authors.initials: PG
authors.lastname: Robey
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D019175:DNA Methylation; D005260:Female; D018392:Genomic Imprinting; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism / Q000502:physiology*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D009569:Nitric Oxide / Q000378:metabolism; D059467:Transcriptome
chemical_list: D008565:Membrane Proteins; C490237:NNAT protein, human; D009419:Nerve Tissue Proteins; C490239:Nnat protein, mouse; D009569:Nitric Oxide