title: Anti-hyperglycemic effect, inhibition of inflammatory cytokines expression, and histopathology profile in streptozotocin-induced diabetic rats treated with Arracacia tolucensis aerial-parts extracts.
abstract: BACKGROUND
  Arracacia tolucensis is a medicinal plant used in northeast of Mexico as a remedy to treat people with Diabetes mellitus (DM); however, there are no scientific studies that support this information. Thus, we evaluated the anti-hyperglycemic effect of the hexane, ethyl acetate and ethanol extracts from aerial parts in streptozotocin-induced diabetic rats.
  
  
  METHODS
  DM was induced in Wistar male rats by single intraperitoneal injection of streptozotocin (STZ 50mg/kg). After STZ-induction, hyperglycemic rats were treated with all three extracts orally at a single dose (250 mg/kg) each 48 h for 21 days. Glibenclamide (1mg/kg) was used as a reference drug. The fasting blood glucose levels, the hematic biometry and biochemical profiles, and the inhibition of inflammatory cytokines expression were estimated. Histopathology analysis of pancreas, liver, spleen, and kidney tissue was carried out.
  
  
  RESULTS
  Ours results showed that ethyl acetate extract decreased blood glucose levels significantly (75%, p< 0.05) when compared to diabetic rats and controlled the body weight loss; the lipids level did not change, but the enzyme levels of aspartate aminotransferase and alanine aminotransferase decreased significantly (60.83% and 66.16%, respectively, p< 0.05) and inhibited the expression of inflammatory cytokines,with respect to diabetic rats. Histopathology injury was not observed; by contrast repair of islet of Langerhans was exhibited.
  
  
  CONCLUSIONS
  These results validate the use of Arracacia tolucensis as a treatment against DM and suggests it is suitable to continue studies for its safe therapeutic use.
authors.affiliation: Laboratorio de Biología Celular y Productos Naturales, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Guillermo Massieu Helguera 239, Col. La Escalera, C.P. 07320 México, D.F., México.
authors.forename: Mary Carmen
authors.initials: MC
authors.lastname: García-Galicia
authors.affiliation: Departamento de Química Orgánica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Col. Santo Tomás, C.P. 11340 México, D.F., México.
authors.forename: Eleuterio
authors.initials: E
authors.lastname: Burgueño-Tapia
authors.affiliation: Centro Universitario de Diagnóstico y Laboratorio de Inmunología de la Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Av. 1° de Mayo s/n, Col. Santa María Las Torres, C.P. 54740 Cuautitlán, Estado de México, México.
authors.forename: Andrés
authors.initials: A
authors.lastname: Romero-Rojas
authors.affiliation: Laboratorio de Biología Celular y Productos Naturales, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Guillermo Massieu Helguera 239, Col. La Escalera, C.P. 07320 México, D.F., México.
authors.forename: Julio César
authors.initials: JC
authors.lastname: García-Zebadúa
authors.affiliation: Laboratorio de Biología Celular y Productos Naturales, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Guillermo Massieu Helguera 239, Col. La Escalera, C.P. 07320 México, D.F., México.
authors.forename: Jorge
authors.initials: J
authors.lastname: Cornejo-Garrido
authors.affiliation: Laboratorio de Biología Celular y Productos Naturales, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Guillermo Massieu Helguera 239, Col. La Escalera, C.P. 07320 México, D.F., México. Electronic address: dra_cynthia@hotmail.com.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Ordaz-Pichardo
mesh_terms: D000818:Animals; D019661:Apiaceae / Q000737:chemistry*; D001786:Blood Glucose / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D020834:Ethnopharmacology; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D008800:Mexico; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000737:chemistry; D013311:Streptozocin
chemical_list: D001786:Blood Glucose; D016207:Cytokines; D007004:Hypoglycemic Agents; D018836:Inflammation Mediators; D010936:Plant Extracts; D012997:Solvents; D013311:Streptozocin
keywords: Anti-hyperglycemic; Arracacia tolucensis; Diabetes mellitus; Herbal extracts