title: Keratin 8 is involved in hepatitis B virus replication.
abstract: Hepatitis B virus (HBV) infection can result in fatal liver diseases, including cirrhosis or liver failure, and its replication and pathogenesis depend on the critical interplay between viral and host factors. This study investigated HBV replication-related host proteins and the effect of candidate proteins on HBV replication. Isobaric tags for relative and absolute quantitation (iTRAQ) were used to measure HBV replication-related proteins in HepG2 cells and HepG2.2.15 cells. KRT8 was up-regulated in HepG2.2.15 cells but not in HepG2 cells, and KRT8 was overexpressed in an HBV-infected patient's liver tissue. This result suggested that KRT8 is involved in HBV replication. To further clarify the relationship between KRT8 and HBV replication, KRT8 gene expression was inhibited by siRNA. The silencing of KRT8 mildly suppressed HBV replication. Moreover, overexpressed KRT8 significantly increased HBV replication, and the inhibition of HBV DNA did not suppress KRT8 expression. Thus, the host protein KRT8 is involved in the replication of HBV DNA, and it dramatically enhances HBV replication.
authors.affiliation: Department of Infectious Diseases, Institute for Viral Hepatitis, Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhong
authors.forename: Xuan
authors.initials: X
authors.lastname: An
authors.forename: Yi-Xuan
authors.initials: YX
authors.lastname: Yang
authors.forename: Huai-Dong
authors.initials: HD
authors.lastname: Hu
authors.forename: Hong
authors.initials: H
authors.lastname: Ren
authors.forename: Peng
authors.initials: P
authors.lastname: Hu
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics; D015870:Gene Expression; D056945:Hep G2 Cells; D006509:Hepatitis B / Q000821:virology; D006515:Hepatitis B virus / Q000254:growth & development*; D006801:Humans; D053533:Keratin-8 / Q000096:biosynthesis / Q000235:genetics*; D008099:Liver / Q000473:pathology / Q000821:virology; D034622:RNA Interference; D034741:RNA, Small Interfering; D014779:Virus Replication / Q000235:genetics*
chemical_list: D004279:DNA, Viral; C426203:KRT8 protein, human; D053533:Keratin-8; D034741:RNA, Small Interfering
keywords: Keratin; hepatitis B virus; quantitative proteomics; replication