title: SPECT vs. PET monitoring of bone defect healing and biomaterial performance in vivo.
abstract: Quantification of the bone healing processes by X-ray-based methods becomes inaccurate in the presence of radiopaque synthetic materials. In this study, single photon emission computed tomography (SPECT) and positron emission tomography (PET) were compared as alternatives to follow in vivo bone healing in a rat calvarial defect model. SPECT/computed tomography (CT) following administration of99mtechnetium-labelled hydroxymethylene diphosphonate (99mTc-HDP) and PET/CT data with18F-fluoride were acquired up to 10 weeks after surgery. New bone formation was then confirmed by histology. Computed tomography scans allowed visualization of untreated bone defect healing; however, no information was gathered in presence of the ceramic. Positron emission tomography provided superior data compared with SPECT. The18F-fluoride uptake increased significantly up to 4 weeks after surgery, declining thereafter until the last time-point. In vivo performances of porous versus dense ceramic scaffolds were also evaluated by PET, with a significantly higher uptake registered within the porous scaffolds. In conclusion, PET is a valuable tool for qualitative/quantitative follow-up of bone healing around radiopaque bone substitutes in vivo. Copyright © 2014 John Wiley & Sons, Ltd.
authors.affiliation: Department of Biomaterials, Radboud University Nijmegen Medical Centre, Nijmegen, the Netherlands.
authors.forename: Manuela
authors.initials: M
authors.lastname: Ventura
authors.affiliation: Department of Nuclear Medicine, Radboud University Nijmegen Medical Centre, Nijmegen, the Netherlands.
authors.forename: Gerben M
authors.initials: GM
authors.lastname: Franssen
authors.affiliation: Department of Urology, Radboud University Nijmegen Medical Centre, Nijmegen, the Netherlands.
authors.forename: Egbert
authors.initials: E
authors.lastname: Oosterwijk
authors.affiliation: Department of Nuclear Medicine, Radboud University Nijmegen Medical Centre, Nijmegen, the Netherlands.
authors.forename: Otto C
authors.initials: OC
authors.lastname: Boerman
authors.affiliation: Department of Biomaterials, Radboud University Nijmegen Medical Centre, Nijmegen, the Netherlands. j.jansen@dent.umcn.nl.
authors.forename: John A
authors.initials: JA
authors.lastname: Jansen
authors.affiliation: Department of Biomaterials, Radboud University Nijmegen Medical Centre, Nijmegen, the Netherlands.
authors.forename: X Frank
authors.initials: XF
authors.lastname: Walboomers
mesh_terms: D000818:Animals; D002516:Ceramics / Q000737:chemistry*; D004164:Diphosphonates / Q000494:pharmacology; D005459:Fluorides / Q000494:pharmacology; D008297:Male; D010012:Osteogenesis; D049268:Positron-Emission Tomography; D051381:Rats; D017208:Rats, Wistar; D000072098:Single Photon Emission Computed Tomography Computed Tomography; D012886:Skull / Q000000981:diagnostic imaging / Q000378:metabolism / Q000601:surgery; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D004164:Diphosphonates; C029285:hydroxymethanediphosphonic acid; D005459:Fluorides
keywords: bone tissue engineering; calcium phosphate cement; computed tomography; positron emission tomography; single-photon emission computed tomography