title: Influence of the vitrification solution on the angiogenic factors in vitrificated mouse ovarian tissue.
abstract: OBJECTIVE
  To investigate the effect of the dimethyl sulfoxide (DMSO) and EFS-40 during vitrification on the expression of angiogenic factors in vitrified mouse ovarian tissue.
  
  
  METHODS
  The ovarian tissues were obtained from 5 or 6 weeks aged ICR mouse. Ovarian tissues were divided into four groups: ovarian tissue without cryopreservation (control, group I), ovarian tissue vitrified with 15% DMSO (group II), ovarian tissue vitrified with EFS-40 (group III), and ovarian tissue slowly frozen with 10% DMSO (group IV). Thawing was carried out at room temperature. Levels of messenger RNA (mRNA) and protein for vascular endothelial growth factor-A (VEGF-A) and angiopoietin-2 (Angpt-2) were checked in ovarian tissues of four groups recovered on day 7 after cryopreservation. Reverse transcription-polymerase chain reaction and Western blot analysis were used to identify the levels of angiogenic factors in mouse ovarian tissues.
  
  
  RESULTS
  Levels of mRNA and protein for VEGF-A and Angpt-2 were significantly decreased in cryopreserved group (group II, III and IV) than control group (group I) (P< 0.05). The significant differences of levels of mRNA and protein for VEGF-A and Angpt-2 between cryopreservation methods were observed (P< 0.05). Group III showed highest expression of mRNA and protein for VEFG-A and Angpt-2 than other cryopreservation groups (P< 0.05).
  
  
  CONCLUSIONS
  These findings suggest that EFS-40 is more efficient vitrification solution for preservation of angiogenic factors than 15% DMSO during vitrification of mouse ovarian tissue. Future studies should investigate to improve the vitrification solution for ovarian tissue vitrification.
authors.affiliation: Department of Obstetrics and Gynecology, Gyeongsang National University School of Medicine, Jinju, Korea. ; Institute of Health Science, Gyeongsang National Universtiy, Jinju, Korea.
authors.forename: Won-Jun
authors.initials: WJ
authors.lastname: Choi
authors.affiliation: Department of Obstetrics and Gynecology, Gyeongsang National University School of Medicine, Jinju, Korea.
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Obstetrics and Gynecology, Gyeongsang National University School of Medicine, Jinju, Korea.
authors.forename: Mi-Hyun
authors.initials: MH
authors.lastname: Park
authors.affiliation: Department of Obstetrics and Gynecology, Gyeongsang National University School of Medicine, Jinju, Korea.
authors.forename: In-Young
authors.initials: IY
authors.lastname: Choi
authors.affiliation: Department of Obstetrics and Gynecology, Gyeongsang National University School of Medicine, Jinju, Korea. ; Institute of Health Science, Gyeongsang National Universtiy, Jinju, Korea.
authors.forename: Ji-Kwon
authors.initials: JK
authors.lastname: Park
authors.affiliation: Department of Obstetrics and Gynecology, Gyeongsang National University School of Medicine, Jinju, Korea. ; Institute of Health Science, Gyeongsang National Universtiy, Jinju, Korea.
authors.forename: Jeong-Kyu
authors.initials: JK
authors.lastname: Shin
authors.affiliation: Department of Obstetrics and Gynecology, Gyeongsang National University School of Medicine, Jinju, Korea. ; Institute of Health Science, Gyeongsang National Universtiy, Jinju, Korea.
authors.forename: Soon-Ae
authors.initials: SA
authors.lastname: Lee
authors.affiliation: Department of Obstetrics and Gynecology, Gyeongsang National University School of Medicine, Jinju, Korea. ; Institute of Health Science, Gyeongsang National Universtiy, Jinju, Korea.
authors.forename: Won-Young
authors.initials: WY
authors.lastname: Paik
authors.affiliation: Department of Obstetrics and Gynecology, Gyeongsang National University School of Medicine, Jinju, Korea. ; Institute of Health Science, Gyeongsang National Universtiy, Jinju, Korea.
authors.forename: Jong-Hak
authors.initials: JH
authors.lastname: Lee
keywords: Angiopoietin-2; Ovary; Vascular endothelial growth factor A; Vitrification