title: Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection.
abstract: A new method for rapid determination of toxic metabolites after methanol and ethylene glycol intoxication - oxalate, formate and glycolate in various body fluid samples (blood serum, saliva, urine, exhaled breath condensate) by capillary electrophoresis with contactless conductometric detection was developed. A selective separation of the three target analytes from other constituents present in the analyzed biological matrices was achieved in less than 6min in a fused silica capillary of 25μm I.D. using an electrolyte comprising 50mM l-histidine and 50mM 2-(N-morpholino)ethanesulfonic acid at pH 6.1. The only sample preparation was dilution with deionized water. The limits of detection were 0.4, 0.6 and 1.3μM and limits of quantitation 1.3, 1.9 and 4.2μM for oxalate, formate and glycolate, respectively. The method provides a simple and rapid diagnostic test in suspected intoxication and is able to distinguish the ingested liquid, based on its metabolite trace. The method presents a fast screening tool that can be applicable in clinical practice.
authors.affiliation: Bioanalytical Instrumentation, CEITEC, Masaryk University, Veveří 97, 60200 Brno, Czech Republic; Institute of Chemistry, Masaryk University, Kotlářská 2, 61137 Brno, Czech Republic. Electronic address: petr.kuban@ceitec.muni.cz.
authors.forename: Petr
authors.initials: P
authors.lastname: Kubáň
authors.affiliation: Institute of Chemistry, Masaryk University, Kotlářská 2, 61137 Brno, Czech Republic.
authors.forename: Pavol
authors.initials: P
authors.lastname: Ďurč
authors.affiliation: Institute of Chemistry, Masaryk University, Kotlářská 2, 61137 Brno, Czech Republic.
authors.forename: Miroslava
authors.initials: M
authors.lastname: Bittová
authors.affiliation: Bioanalytical Instrumentation, CEITEC, Masaryk University, Veveří 97, 60200 Brno, Czech Republic.
authors.forename: František
authors.initials: F
authors.lastname: Foret
mesh_terms: D001826:Body Fluids / Q000737:chemistry*; D004573:Electrolytes; D019075:Electrophoresis, Capillary / Q000379:methods*; D005561:Formates / Q000302:isolation & purification*; D006016:Glycolates / Q000302:isolation & purification*; D006801:Humans
chemical_list: D004573:Electrolytes; D005561:Formates; D006016:Glycolates; C031149:glycolic acid; C030544:formic acid
keywords: Body fluid samples; Capillary electrophoresis; Contactless conductivity detection; Ethylene glycol intoxication; Methanol intoxication; Toxic metabolites