title: Structural basis for regulation of ribosomal RNA 2'-o-methylation.
abstract: Methods that interrogate different, yet overlapping structural-size ranges were integrated to gain high-resolution information on the Pyrococcus furiosus box C/D ribonucleoprotein complex. The new structure shows how 2'-O-methylation of RNA substrates through four active methyltransferases occurs in a strictly sequential manner.
authors.affiliation: Institut für Organische Chemie und Chemische Biologie, Biomolekulares Magnetresonanz-Zentrum (BMRZ), Goethe-Universität Frankfurt, Max-von-Laue-Strasse 7, 60438 Frankfurt am Main (Germany).
authors.forename: Martin
authors.initials: M
authors.lastname: Hengesbach
authors.forename: Harald
authors.initials: H
authors.lastname: Schwalbe
mesh_terms: D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008958:Models, Molecular; D059370:RNA Folding; D012335:RNA, Ribosomal / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D012335:RNA, Ribosomal
keywords: 2′-O-methylation; NMR spectroscopy; RNA modification; ribosome biogenesis; small-angle neutron scattering