title: Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors.
abstract: In-Fusion™ cloning is a flexible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in Escherichia coli of constructs generated by In-Fusion™ is also outlined.
authors.affiliation: Oxford Protein Production Facility-UK, Oxfordshire, UK.
authors.forename: Louise E
authors.initials: LE
authors.lastname: Bird
authors.forename: Heather
authors.initials: H
authors.lastname: Rada
authors.forename: John
authors.initials: J
authors.lastname: Flanagan
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Diprose
authors.forename: Robert J C
authors.initials: RJ
authors.lastname: Gilbert
authors.forename: Raymond J
authors.initials: RJ
authors.lastname: Owens
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic
chemical_list: D017931:DNA Primers