title: The first salamander defensin antimicrobial peptide.
abstract: Antimicrobial peptides have been widely identified from amphibian skins except salamanders. A novel antimicrobial peptide (CFBD) was isolated and characterized from skin secretions of the salamander, Cynops fudingensis. The cDNA encoding CFBD precursor was cloned from the skin cDNA library of C. fudingensis. The precursor was composed of three domains: signal peptide of 17 residues, mature peptide of 41 residues and intervening propeptide of 3 residues. There are six cysteines in the sequence of mature CFBD peptide, which possibly form three disulfide-bridges. CFBD showed antimicrobial activities against Staphylococcus aureus, Bacillus subtilis, Candida albicans and Escherichia coli. This peptide could be classified into family of β-defensin based on its sequence similarity with β-defensins from other vertebrates. Evolution analysis indicated that CFBD was close to fish β-defensin. As far as we know, CFBD is the first β-defensin antimicrobial peptide from salamanders.
authors.affiliation: Life Sciences College of Nanjing Agricultural University, Nanjing,Jiangsu, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Meng
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, China.
authors.forename: Shilong
authors.initials: S
authors.lastname: Yang
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, China.
authors.forename: Chuanbin
authors.initials: C
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, China.
authors.forename: Mingqiang
authors.initials: M
authors.lastname: Rong
authors.affiliation: Life Sciences College of Nanjing Agricultural University, Nanjing,Jiangsu, China ; Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, China.
authors.forename: Ren
authors.initials: R
authors.lastname: Lai
mesh_terms: D029845:Amphibian Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D003001:Cloning, Molecular; D004912:Erythrocytes / Q000187:drug effects; D015723:Gene Library; D053878:Hemolytic Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D010802:Phylogeny; D011817:Rabbits; D020539:Sequence Analysis, Protein; D013211:Staphylococcus aureus / Q000187:drug effects; D014562:Caudata / Q000235:genetics* / Q000378:metabolism; D023083:beta-Defensins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*
chemical_list: D029845:Amphibian Proteins; D000890:Anti-Infective Agents; D053878:Hemolytic Agents; D023083:beta-Defensins