title: Evaluation of Brugia malayi sheath protein (Shp-1) as a diagnostic antigen for human lymphatic filariasis.
abstract: Lymphatic filariasis is the second leading cause of permanent long-term disability globally and control of this disease needs efficient diagnostic methods. In this study, abundantly expressing microfilarial sheath protein (Shp-1) from Brugia malayi was characterized as a filarial diagnostic candidate using samples from different clinical population. Monoclonal antibodies were developed against E. coli expressed recombinant Shp-1 in order to assess its efficiency in filarial antigen detection assay system. Endemic Normal (EN, n = 170), asymptomatic microfilaeremics (MF, n = 65), symptomatic chronic pathology (CP, n = 45) and non endemic normal (NEN, n = 10) sera were analyzed by antigen capture enzyme-linked immunosorbent assay. Of the 290 individuals, all MF individuals (both brugian and bancroftian) were positive in this assay followed by CP and EN. When compared with SXP-1 and Og4C3 antigen assays, all assays detected Wb MF correctly, Bm MF was detected by Shp-1 and SXP-1 assays, and only Shp-1 was able to detect EN (12%) and CP (29%). Results showed that this assay may be useful for monitoring prior to mass drug administration.
authors.affiliation: Centre for Biotechnology, Anna University, Chennai, Tamil Nadu, India. Electronic address: jai.auc@gmail.com.
authors.forename: Jeya Prita Parasurama
authors.initials: JP
authors.lastname: Jawaharlal
authors.affiliation: Centre for Biotechnology, Anna University, Chennai, Tamil Nadu, India. Electronic address: jairavibt@gmail.com.
authors.forename: Rajendran
authors.initials: R
authors.lastname: Ravishankaran
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Bangalore, Karnataka, India. Electronic address: radhikans@gmail.com.
authors.forename: Radhika Nagamangalam
authors.initials: RN
authors.lastname: Shridharan
authors.affiliation: Centre for Biotechnology, Anna University, Chennai, Tamil Nadu, India. Electronic address: vicchuans@gmail.com.
authors.forename: Ansel Vishal
authors.initials: AV
authors.lastname: Lawrence
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Bangalore, Karnataka, India. Electronic address: anjali@biochem.iisc.ernet.in.
authors.forename: Anjali Anoop
authors.initials: AA
authors.lastname: Karande
authors.affiliation: Centre for Biotechnology, Anna University, Chennai, Tamil Nadu, India. Electronic address: pkaliraj@annauniv.edu.
authors.forename: Kaliraj
authors.initials: K
authors.lastname: Perumal
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000097:blood* / Q000302:isolation & purification; D001483:Base Sequence; D017178:Brugia malayi; D004605:Elephantiasis, Filarial / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D015801:Helminth Proteins / Q000097:blood* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014958:Wuchereria bancrofti
chemical_list: D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D015801:Helminth Proteins; D011994:Recombinant Proteins
keywords: Brugia malayi; ELISA; Lymphatic filariasis; Monoclonal antibodies; Sheath protein; Shp-1; Wuchereria bancrofti