title: Cell and tissue-autonomous development of the circadian clock in mouse embryos.
abstract: The emergence of the circadian rhythm is a dramatic and physiologically essential event for mammals to adapt to daily environmental cycles. It has been demonstrated that circadian rhythms develop during the embryonic stage even when the maternal central pacemaker suprachiasmatic nucleus has been disrupted. However, the mechanisms controlling development of the circadian clock are not yet fully understood. Here, we show that the circadian molecular oscillation in primary dispersed embryonic cells and explanted salivary glands obtained from mPER2(Luc) mice embryos developed cell- or tissue-autonomously even in tissue culture conditions. Moreover, the circadian clock in the primary mPER2(Lu)(c) fibroblasts could be reprogrammed by the expression of the reprogramming factors. These findings suggest that mammalian circadian clock development may interact with cellular differentiation mechanisms.
authors.affiliation: Department of Physiology and Systems Bioscience, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan; Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, 602-8566, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Inada
authors.affiliation: Laboratory of Oral Chronobiology, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, Japan; Department of Oral-Facial Disorders, Osaka University Graduate School of Dentistry, Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Uchida
authors.affiliation: Department of Physiology and Systems Bioscience, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Umemura
authors.affiliation: Laboratory of Oral Chronobiology, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Nakamura
authors.affiliation: Department of Oral-Facial Disorders, Osaka University Graduate School of Dentistry, Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Sakai
authors.affiliation: Department of Physiology and Systems Bioscience, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
authors.forename: Nobuya
authors.initials: N
authors.lastname: Koike
authors.affiliation: Department of Physiology and Systems Bioscience, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan. Electronic address: kyagita@koto.kpu-m.ac.jp.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yagita
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000096:biosynthesis; D057906:Circadian Clocks / Q000235:genetics*; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000235:genetics*; D005347:Fibroblasts; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D009043:Motor Activity; D056950:Period Circadian Proteins / Q000096:biosynthesis*; D014018:Tissue Distribution / Q000235:genetics
chemical_list: D018797:Cell Cycle Proteins; D056950:Period Circadian Proteins
keywords: Cellular differentiation; Circadian rhythm; Development; Embryo