title: Chromatin modification mapping in nanochannels.
abstract: We report the simultaneous mapping of multiple histone tail modifications on chromatin that has been confined to nanofluidic channels. In these channels, chromatin is elongated, and histone modification can be detected using fluorescently tagged monoclonal antibodies. Using reconstituted chromatin with three distinct histone sources and two histone tail modification probes (H3K4me3 and H3K9ac), we were able to distinguish chromatin from the different sources. Determined ratios of the two modifications were consistent with the bulk composition of histone mixtures. We determined that the major difficulty in transitioning the mapping method to site-specific profiling within single genomic molecules is the interference of naturally aggregating, off-the shelf antibodies with the internal structure of chromatin.
authors.affiliation: Department of Physics, North Carolina State University, Raleigh, North Carolina 27695, USA.
authors.forename: Shuang Fang
authors.initials: SF
authors.lastname: Lim
authors.affiliation: Department of Physics, North Carolina State University, Raleigh, North Carolina 27695, USA.
authors.forename: Alena
authors.initials: A
authors.lastname: Karpusenko
authors.affiliation: Department of Physics, North Carolina State University, Raleigh, North Carolina 27695, USA.
authors.forename: Ansel L
authors.initials: AL
authors.lastname: Blumers
authors.affiliation: Department of Physics, North Carolina State University, Raleigh, North Carolina 27695, USA.
authors.forename: Diana E
authors.initials: DE
authors.lastname: Streng
authors.affiliation: Department of Physics, North Carolina State University, Raleigh, North Carolina 27695, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Riehn