title: Performance of an in situ formed bioactive hydrogel dressing from a PEG-based hyperbranched multifunctional copolymer.
abstract: Hydrogel dressings have been widely used for wound management due to their ability to maintain a hydrated wound environment, restore the skin's physical barrier and facilitate regular dressing replacement. However, the therapeutic functions of standard hydrogel dressings are restricted. In this study, an injectable hybrid hydrogel dressing system was prepared from a polyethylene glycol (PEG)-based thermoresponsive hyperbranched multiacrylate functional copolymer and thiol-modified hyaluronic acid in combination with adipose-derived stem cells (ADSCs). The cell viability, proliferation and metabolic activity of the encapsulated ADSCs were studied in vitro, and a rat dorsal full-thickness wound model was used to evaluate this bioactive hydrogel dressing in vivo. It was found that long-term cell viability could be achieved for both in vitro (21days) and in vivo (14days) studies. With ADSCs, this hydrogel system prevented wound contraction and enhanced angiogenesis, showing the potential of this system as a bioactive hydrogel dressing for wound healing.
authors.affiliation: The Charles Institute of Dermatology, School of Medicine and Medical Science, University College Dublin, Dublin, Ireland.
authors.forename: Yixiao
authors.initials: Y
authors.lastname: Dong
authors.affiliation: The Charles Institute of Dermatology, School of Medicine and Medical Science, University College Dublin, Dublin, Ireland.
authors.forename: Waqar U
authors.initials: WU
authors.lastname: Hassan
authors.affiliation: The Charles Institute of Dermatology, School of Medicine and Medical Science, University College Dublin, Dublin, Ireland.
authors.forename: Robert
authors.initials: R
authors.lastname: Kennedy
authors.affiliation: The Charles Institute of Dermatology, School of Medicine and Medical Science, University College Dublin, Dublin, Ireland.
authors.forename: Udo
authors.initials: U
authors.lastname: Greiser
authors.affiliation: Network of Excellence for Functional Biomaterials, National University of Ireland, Galway, Ireland.
authors.forename: Abhay
authors.initials: A
authors.lastname: Pandit
authors.affiliation: Anatomy Department, National University of Ireland, Galway, Ireland. Electronic address: yolanda.garcia@nuigalway.ie.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Garcia
authors.affiliation: The Charles Institute of Dermatology, School of Medicine and Medical Science, University College Dublin, Dublin, Ireland. Electronic address: wenxin.wang@ucd.ie.
authors.forename: Wenxin
authors.initials: W
authors.lastname: Wang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D001458:Bandages; D001672:Biocompatible Materials / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D006820:Hyaluronic Acid / Q000494:pharmacology; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D007150:Immunohistochemistry; D007249:Inflammation / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000473:pathology; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects; D011092:Polyethylene Glycols / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; D006820:Hyaluronic Acid
keywords: Adipose derived stem cells; Hydrogel dressing; In situ cross-link; PEG based hyperbranched polymer; Wound healing