title: Imidazolium-based iodoacetamide functional tags: design, synthesis, and property study for cysteinyl-peptide analysis by mass spectrometry.
abstract: New types of imidazolium-based iodoacetamide tags were designed, synthesized and further exploited for cysteinyl-peptide analysis with superior labeling efficiency, high stability, improved ionization efficiency, and increased charge states by mass spectrometry. For the first time, the effects of these kinds of tags on the mass spectrometry performance of the derivatized peptides were investigated, which is of great importance to help us design more efficient tags for the analysis of peptides or proteins, especially for those with low abundance.
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education & College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China. xiaoqiao@hbu.edu.cn.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Qiao
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Guangyue
authors.initials: G
authors.lastname: Li
authors.forename: Hongyuan
authors.initials: H
authors.lastname: Yan
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D003545:Cysteine / Q000032:analysis / Q000737:chemistry; D007093:Imidazoles / Q000737:chemistry; D048288:Imidazolines / Q000737:chemistry; D007460:Iodoacetamide / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000737:chemistry
chemical_list: C583483:1-(3-aminopropyl)-3-butylimidazolium; D007093:Imidazoles; D048288:Imidazolines; D010455:Peptides; D011506:Proteins; D003545:Cysteine; D007460:Iodoacetamide