title: Regeneration of chronic myocardial infarction by injectable hydrogels containing stem cell homing factor SDF-1 and angiogenic peptide Ac-SDKP.
abstract: Regeneration of chronic myocardial infarction (CMI) is one of the challenging issues due to its limited regeneration activity compared to acute or sub-acute stage. In this study, we examined whether combination of stem cell homing factor (SDF-1) and angiogenic peptides (Ac-SDKP) injected with biomimetic hydrogels promote regeneration of cardiac function in a CMI model. We evaluated the regeneration of chronically infarcted myocardium using injectable biomimetic hydrogels containing two therapeutic factors; stromal-derived factor-1 (SDF-1) and Ac-SDKP for stem cell homing and angiogenesis, respectively. Injection of the two therapeutic factors into the infarct region of the left ventricle showed that the biomimetic hydrogels containing two therapeutic factor exhibited significantly improved left ventricle function, increased angiogenesis, decreased infarct size and greatest wall thickness within the infarct region at 4 weeks post-treatment. From these results, it is clear that hydrogels containing two therapeutic factors showed synergistic effects on regeneration in the chronic heart failure model. In conclusion, these results suggest that combination of stem cell homing factor with angiogenic peptides recruit stem cells to the microenvironments, increase the expression of angiogenic genes, enhance the matured vessel formation and improve the cardiac function in chronic MI.
authors.affiliation: Korea Artificial Organ Center, Korea University, Seoul 136-705, Republic of Korea.
authors.forename: Myeongjin
authors.initials: M
authors.lastname: Song
authors.affiliation: Korea Artificial Organ Center, Korea University, Seoul 136-705, Republic of Korea; Department of Biomedical Engineering, College of Medicine, Korea University, Seoul 136-705, Republic Korea.
authors.forename: Hwanseok
authors.initials: H
authors.lastname: Jang
authors.affiliation: Korea Artificial Organ Center, Korea University, Seoul 136-705, Republic of Korea.
authors.forename: Jaeyeon
authors.initials: J
authors.lastname: Lee
authors.affiliation: Biomaterials Research Center, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea.
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Biomaterials Research Center, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea.
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Biomedical Engineering, College of Medicine, Korea University, Seoul 136-705, Republic Korea; Department of Thoracic and Cardiovascular Surgery, College of Medicine, Korea University, Seoul 136-705, Republic of Korea.
authors.forename: Kyung
authors.initials: K
authors.lastname: Sun
authors.affiliation: Korea Artificial Organ Center, Korea University, Seoul 136-705, Republic of Korea; Department of Biomedical Engineering, College of Medicine, Korea University, Seoul 136-705, Republic Korea. Electronic address: ydpark@kumc.or.kr.
authors.forename: Yongdoo
authors.initials: Y
authors.lastname: Park
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000235:genetics / Q000494:pharmacology*; D004357:Drug Synergism; D015870:Gene Expression; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D009203:Myocardial Infarction / Q000628:therapy*; D009206:Myocardium / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000187:drug effects; D009842:Oligopeptides / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019718:Receptors, CXCR4 / Q000378:metabolism; D012038:Regeneration / Q000187:drug effects*; D059467:Transcriptome; D016277:Ventricular Function, Left
chemical_list: D001672:Biocompatible Materials; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; C486264:Cxcr4 protein, rat; D020100:Hydrogels; D009842:Oligopeptides; D019718:Receptors, CXCR4; C058504:goralatide
keywords: Ac-SDKP; Chronic heart failure; Controlled release system; Hydrogel; Matrix metalloproteinase (MMP); SDF-1