title: Simple and rapid detection of HLA-A*31:01 for prediction of carbamazepine-induced hypersensitivity using loop-mediated isothermal amplification method.
abstract: BACKGROUND
  Carbamazepine (CBZ), which is widely used in management of epilepsy or neuropathic pain, causes fatal severe cutaneous adverse reactions (SCARs). CBZ-induced SCARs are known to occur in strong association with human leukocyte antigen (HLA)-A*31:01 in Japanese and European populations. HLA genotyping is currently used to detect human HLA-A*31:01.
  
  
  OBJECTIVE
  To establish a simple and rapid screening assay specific for HLA-A*31:01, the loop-mediated isothermal amplification (LAMP) method was employed on a sample Japanese population.
  
  
  METHODS
  A set of LAMP primers targeting exon 2 of HLA-A*31:01 were designed. Thirty-two clinical samples including the representative HLA-A allele in Japan were used to assess the specificity of LAMP primers in the detection of HLA-A*31:01.
  
  
  RESULTS
  The HLA-A*31:01-specific LAMP assay showed consistency with polymerase chain reaction reverse sequence-specific oligonucleotide probe (PCR-rSSO) and polymerase chain reaction-sequence based typing (PCR-SBT) results.
  
  
  CONCLUSIONS
  High sensitivity and specificity of the HLA-A*31:01 LAMP assay was confirmed. Considering its convenience, the assay can be widely used to screen patients at high genetic risk of CBZ-induced SCARs.
authors.affiliation: Department of Dermatology, Shimane University Hospital, Izumo, Japan. Electronic address: ofcourse@med.shimane-u.ac.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Niihara
authors.affiliation: Center for Community-Based Health Research and Education (COHRE), Organization for the Promotion of Project Research, Shimane University, Izumo, Japan.
authors.forename: Kunie
authors.initials: K
authors.lastname: Kohno
authors.affiliation: Department of Pediatrics, Shimane University Hospital, Izumo, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Taketani
authors.affiliation: Department of Dermatology, Shimane University Hospital, Izumo, Japan.
authors.forename: Sakae
authors.initials: S
authors.lastname: Kaneko
authors.affiliation: Section of Nephrology, Department of Internal Medicine, Shimane University Hospital, Izumo, Japan.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Ito
authors.affiliation: Section of Cardiovascular Internal Medicine, Department of Internal Medicine, Shimane University Hospital, Izumo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Sugamori
authors.affiliation: Section of Cardiovascular Internal Medicine, Department of Internal Medicine, Shimane University Hospital, Izumo, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Takahashi
authors.affiliation: Department of Psychiatry, Shimane University Hospital, Izumo, Japan.
authors.forename: Tuyoshi
authors.initials: T
authors.lastname: Miyaoka
authors.affiliation: Department of Psychiatry, Shimane University Hospital, Izumo, Japan.
authors.forename: Shihoh
authors.initials: S
authors.lastname: Okazaki
authors.affiliation: Department of Psychiatry, Shimane University Hospital, Izumo, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Yasuda
authors.affiliation: Department of Psychiatry, Shimane University Hospital, Izumo, Japan.
authors.forename: Motohide
authors.initials: M
authors.lastname: Furuya
authors.affiliation: Department of Psychiatry, Shimane University Hospital, Izumo, Japan.
authors.forename: Michiharu
authors.initials: M
authors.lastname: Nagahama
authors.affiliation: Department of Dermatology, Shimane University Hospital, Izumo, Japan.
authors.forename: Eishin
authors.initials: E
authors.lastname: Morita
mesh_terms: D000483:Alleles; D000927:Anticonvulsants / Q000009:adverse effects; D001483:Base Sequence; D002220:Carbamazepine / Q000009:adverse effects*; D017931:DNA Primers / Q000235:genetics; D004342:Drug Hypersensitivity / Q000235:genetics* / Q000401:mortality; D005787:Gene Frequency; D005838:Genotype; D015234:HLA-A Antigens / Q000032:analysis*; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012867:Skin / Q000187:drug effects
chemical_list: D000927:Anticonvulsants; D017931:DNA Primers; D015234:HLA-A Antigens; C506273:HLA-A*31:01 antigen; D002220:Carbamazepine
keywords: Carbamazepine; Fatal severe cutaneous adverse reactions; Human leukocyte antigen-A*31:01; Loop-mediated isothermal amplification