title: [Genetic characterization of human parainfluenza virus 3 circulating in Gansu and Shaanxi Provinces from 2009 to 2011].
abstract: To investigate the genetic characterization of Human parainfluenza virus-3 (HPIV-3) circulating in Gansu and Shaanxi Provinces of China, 719 throat swabs were collected from pediatric patients with acute respiratory infections from 2009-2011. Multiplex RT-PCR was used to screen common respiratory viral pathogens. For HPIV-3-positive specimens, nested RT-PCR was used to amplify the HN gene of HPIV-3. The nucleotides of Hemagglutinin-neuraminidase(HN)gene of 13 HPIV-3 positive strains identified in Gansu and Shaanxi Provinces were successfully sequenced and compared with those downloaded from GenBank. The phylogenetic analysis based on the nucleotides sequence of HN gene showed that 13 HPIV-3 strains belonged to sub-cluster C3 with little sequence variation (overall nucleotide divergence of 0.2%-2.3% and amino acid divergence at 0-1.1%). Compared with the complete gene of HPIV-3 strains from U.S.A., Canada, and Australia, the biggest divergence of the nucleotide and amino acid lovels was 6.0% and 3.4%, respectively. The nucleotide divergence between shaanxi09-2 and shaanxi10-H0091 was 0.9%, while the nucleotide divergence between shaanxi10-H005 and gansull-62110372 was 0.5%, between shaanxi09-2 and BJ/291/09 was 0.6%. However, there was no amino acid divergence among them. It is likely that HPIV-3 virus had been transmitting in Gansu and Shaanxi Provinces for several years. Human parainfluenza virus-3 (HPIV-3) circulated in Gansu and Shaanxi Provinces from 2009 to 2011 belonged to sub-cluster C3.
authors.affiliation: Basic Medical School, Laboratory Center for Medical Science, Lanzhou University, Lanzhou 730000, China. pengzhang86@126.com
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Yi-xin
authors.initials: YX
authors.lastname: Ji
authors.affiliation: National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Song-tao
authors.initials: ST
authors.lastname: Xu
authors.affiliation: Gansu Provincial Center for Disease Control and Prevention, Lanzhou 730000, China.
authors.forename: De-shan
authors.initials: DS
authors.lastname: Yu
authors.affiliation: Shaanxi Provincial Center for Disease Control and Prevention, Xian 710000, China.
authors.forename: Peng-bo
authors.initials: PB
authors.lastname: Yu
authors.affiliation: Gansu Provincial Center for Disease Control and Prevention, Lanzhou 730000, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Shaanxi Provincial Center for Disease Control and Prevention, Xian 710000, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Ai-li
authors.initials: AL
authors.lastname: Cui
authors.affiliation: Basic Medical School, Laboratory Center for Medical Science, Lanzhou University, Lanzhou 730000, China.
authors.forename: Hu-lai
authors.initials: HL
authors.lastname: Wei
authors.affiliation: National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Wen-bo
authors.initials: WB
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002681:China / Q000453:epidemiology; D005260:Female; D014644:Genetic Variation; D015707:HN Protein / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010224:Parainfluenza Virus 3, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D010253:Respirovirus Infections / Q000453:epidemiology / Q000821:virology*; D012621:Seasons; D055815:Young Adult
chemical_list: D015707:HN Protein