title: The effects of silorane composites on levels of cytokines and periodontal parameters.
abstract: OBJECTIVE
  The purpose of this pilot study was to determine the effects of silorane composites on gingival crevicular fluid (GCF) levels of tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6) and IL-8, GCF volume and clinical periodontal parameters in patients with silorane composite restorations before and after restorative treatment.
  
  
  METHODS
  A total of 20 systemically healthy non-smokers, 12 female and 8 male (age range: 24-46 years), presenting with 25 instances of primary dentine caries with subgingival margins were selected for this study. Approval was obtained from the university ethics committee and treatment plans were approved by the patients. GCF samples were obtained with periopaper strips from relevant teeth for IL-6, IL-8 and TNF-α measurements. Each sample was stored at - 80°C and analyzed using the enzyme-linked immunosorbent assay (ELISA) kits. Cavities were prepared according to the common principles for adhesive restorations and restored with a silorane adhesive system (Silorane System Adhesive (3M ESPE) and silorane composite (Filtek Silorane, 3M ESPE). Cytokine levels were reassessed 2 weeks after restorative treatment. Data were analyzed using the independent t-test at a significance level of α =0.05. Associations between parameters were analyzed using Pearson correlation analysis.
  
  
  RESULTS
  A significant increase in gingival index (GI) and plaque index (PI) were observed after 15 days (P < 0.05). GCF volume, IL-6, IL-8 and TNF-α levels exhibited significant differences before and after restorative treatment (P < 0.05). There were strong positive correlations among parameters except for PI/GCF volume and GI/GCF volume.
  
  
  CONCLUSIONS
  Within the limitations of this investigation, silorane composites may have some negative effects on cytokine levels, clinical parameters and GCF volume.
authors.affiliation: Department of Restorative Dentistry, Faculty of Dentistry, Atatürk University, Erzurum, Turkey.
authors.forename: Nurcan Ozakar
authors.initials: NO
authors.lastname: Ilday
authors.affiliation: Department of Restorative Dentistry, Faculty of Dentistry, Atatürk University, Erzurum, Turkey.
authors.forename: Neslihan
authors.initials: N
authors.lastname: Celik
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Atatürk University, Erzurum, Turkey.
authors.forename: Alparslan
authors.initials: A
authors.lastname: Dilsiz
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey.
authors.forename: Hamit Hamit
authors.initials: HH
authors.lastname: Alp
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Atatürk University, Erzurum, Turkey.
authors.forename: Tuba
authors.initials: T
authors.lastname: Aydin
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Atatürk University, Erzurum, Turkey.
authors.forename: Nilgun
authors.initials: N
authors.lastname: Seven
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Atatürk University, Erzurum, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Kiziltunç
keywords: Class II restorations; cytokine; gingival crevicular fluid; gingival index; plaque index; silorane based resin composite