title: AlphaVBeta5 integrins mediates Pseudomonas fluorescens interaction with A549 cells
abstract: Interaction of pathogenic bacteria with human cells is usually an essential step in the infection process. The bacterial invasion is stimulated by microbial binding to mammalian extracellular matrix proteins such as vitronectin, fibronectin or integrins. We have recently shown that some strains isolated from a clinical environment are able to grow at/or above 37°C. In particular, we demonstrated that P. fluorescens AF181 binds specifically to the surface of A549 human respiratory epithelial cells and that adhesiveness modulates the inflammatory response. In this study, the involvement of Alpha(v)Beta5 integrins and its respective natural ligand vitronectin (VN) in P. fluorescens AF181 adherence and invasion was examined. The host cell cytoskeleton and cellular tyrosine kinases seem to be solicited during the P. fluorescens-respiratory cell interaction; consequently, cytochalasin D and genistein decreased the bacterial adherence and internalization. Gene silencing of α(v), β5 integrins and vitronectin reduced P. fluorescens adherence and internalization to A549 cells. Taken together, these findings suggest that Alpha(v)Beta5 integrins and their natural ligand VN are involved in P. fluorescens adherence and invasion in human epithelial cells.
authors.affiliation: Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology, Second University of Naples, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Buommino
authors.affiliation: Department of General Pathology, Faculty of Medicine of Naples, Second University of Naples, Naples, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Di Domenico
authors.affiliation: Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology, Second University of Naples, Italy.
authors.forename: Iole
authors.initials: I
authors.lastname: Paoletti
authors.affiliation: Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology, Second University of Naples, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Fusco
authors.affiliation: Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology, Second University of Naples, Italy.
authors.forename: Vincenza
authors.initials: V
authors.lastname: De Gregorio
authors.affiliation: Department of business management, University of Naples Parthenope, Naples, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Cozza
authors.affiliation: Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology, Second University of Naples, Italy.
authors.forename: Antonietta
authors.initials: A
authors.lastname: Rizzo
authors.affiliation: Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology, Second University of Naples, Italy.
authors.forename: Maria Antonietta
authors.initials: MA
authors.lastname: Tufano
authors.affiliation: Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology, Second University of Naples, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Donnarumma
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D006801:Humans; D008168:Lung / Q000382:microbiology; D011551:Pseudomonas fluorescens / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D019030:Receptors, Vitronectin / Q000502:physiology*
chemical_list: D017931:DNA Primers; D019030:Receptors, Vitronectin; C064660:integrin alphaVbeta5