title: Semi-automatic segmentation and classification of Pap smear cells.
abstract: Cytologic screening has been widely used for detecting the cervical cancers. In this study, a semiautomatic PC-based cellular image analysis system was developed for segmenting nuclear and cytoplasmic contours and for computing morphometric and textual features to train support vector machine (SVM) classifiers to classify four different types of cells and to discriminate dysplastic from normal cells. A software program incorporating function, including image reviewing and standardized denomination of file names, was also designed to facilitate and standardize the workflow of cell analyses. Two experiments were conducted to verify the classification performance. The cross-validation results of the first experiment showed that average accuracies of 97.16% and 98.83%, respectively, for differentiating four different types of cells and in discriminating dysplastic from normal cells have been achieved using salient features (8 for four-cluster and 7 for two-cluster classifiers) selected with SVM recursive feature addition. In the second experiment, 70% (837) of the cell images were used for training and 30% (361) for testing, achieving an accuracy of 96.12% and 98.61% for four-cluster and two-cluster classifiers, respectively. The proposed system provides a feasible and effective tool in evaluating cytologic specimens.
authors.forename: Yung-Fu
authors.initials: YF
authors.lastname: Chen
authors.forename: Po-Chi
authors.initials: PC
authors.lastname: Huang
authors.forename: Ker-Cheng
authors.initials: KC
authors.lastname: Lin
authors.forename: Hsuan-Hung
authors.initials: HH
authors.lastname: Lin
authors.forename: Li-En
authors.initials: LE
authors.lastname: Wang
authors.forename: Chung-Chuan
authors.initials: CC
authors.lastname: Cheng
authors.forename: Tsung-Po
authors.initials: TP
authors.lastname: Chen
authors.forename: Yung-Kuan
authors.initials: YK
authors.lastname: Chan
authors.forename: John Y
authors.initials: JY
authors.lastname: Chiang
mesh_terms: D002467:Cell Nucleus; D002584:Cervix Uteri / Q000166:cytology; D016000:Cluster Analysis; D003584:Cytological Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D007090:Image Interpretation, Computer-Assisted; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D065006:Papanicolaou Test / Q000379:methods*; D015203:Reproducibility of Results; D012984:Software; D060388:Support Vector Machine