title: Influence of plant growth regulators on callus mediated regeneration and secondary metabolites synthesis in Withania somnifera (L.) Dunal.
abstract: Withania somnifera (L.) Dunal, is an important medicinal plant being the source of extremely important compounds like withanolides and withaferin. Influence of different plant growth regulators (PGRs) were evaluated for induction of callus, callus mediated regeneration and production of secondary metabolites in them. Explants for callusing were collected from plants grown in vitro and maximum callusing (98 %) was obtained on MS medium supplemented with a combination of 2,4-dichlorophenoxy acetic acid (2,4-D) (0.5 mg l(-1)) and kinetin (KN) (0.2 mg l(-1)). Among different types of calli, best shoot regeneration was observed on green, compact calli produced on MS medium with a combination of 6-benzylamino purine (BAP) and indole butyric acid (IBA). MS medium supplemented with BAP (2 mg l(-1)) showed highest frequency (98 %) of shoot bud regeneration. The micro-shoots were efficiently rooted on MS media supplemented with 0.5 mg l(-1) IBA. Rooted plants were transferred to soil-vermi-compost (1:3; w/w) medium in greenhouse for acclimatization. Presence of withanolide A and withaferin A in calli was validated through high performance thin layer chromatography (HPTLC). It was interesting to observe that the PGRs showed significant influence on the secondary metabolites production in callus and 2,4-D having the least effect. Histological studies revealed the origin of shoot tip in the callus during regeneration.
authors.affiliation: West Bengal State Council of Science and Technology, Bikash Bhavan, North block, 4th Floor, Salt Lake, Kolkata 700091 India ; Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata, 700019 India.
authors.forename: Nilanjan
authors.initials: N
authors.lastname: Chakraborty
authors.affiliation: West Bengal State Council of Science and Technology, Bikash Bhavan, North block, 4th Floor, Salt Lake, Kolkata 700091 India ; Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata, 700019 India.
authors.forename: Debarupa
authors.initials: D
authors.lastname: Banerjee
authors.affiliation: West Bengal State Council of Science and Technology, Bikash Bhavan, North block, 4th Floor, Salt Lake, Kolkata 700091 India ; Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata, 700019 India.
authors.forename: Moumita
authors.initials: M
authors.lastname: Ghosh
authors.affiliation: Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata, 700019 India.
authors.forename: Prakash
authors.initials: P
authors.lastname: Pradhan
authors.affiliation: Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata, 700019 India.
authors.forename: Namrata Shanu
authors.initials: NS
authors.lastname: Gupta
authors.affiliation: Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata, 700019 India.
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Acharya
authors.affiliation: West Bengal State Council of Science and Technology, Bikash Bhavan, North block, 4th Floor, Salt Lake, Kolkata 700091 India.
authors.forename: Maitreyi
authors.initials: M
authors.lastname: Banerjee
keywords: Callusing; High performance thin layer chromatography; Histology; Plant growth regulator; Shoot bud regeneration; Withaferin A; Withanolide A