title: Rapid detection of Staphylococcus aureus and methicillin resistance in bone and joint infection samples: evaluation of the GeneXpert MRSA/SA SSTI assay.
abstract: The GeneXpert MRSA/SA SSTI assay was compared to conventional cultures to detect Staphylococcus aureus and methicillin-resistance from 91 bone and joint infection samples. Sensitivity and specificity were 94.4% and 100%. Three false-positive results were observed, in fact providing from patients known to be infected by S. aureus on the basis of other concomitant osteoarticular samples, which suggests that PCR was more sensitive than culture. This diagnosis accuracy may help shorten toxic and non-optimal empirical therapies such as glycopeptides in case of methicillin-susceptible strains.
authors.affiliation: International Center for Research in Infectiology, INSERM U1111, Lyon, France; Laboratory of bacteriology, North Lyon University Hospital, Hospices Civils de Lyon, Lyon, France; Department of infectious diseases, North Lyon University Hospital, Hospices Civils de Lyon, Lyon, France; Lyon I Claude Bernard University, Lyon, France. Electronic address: florent.valour@chu-lyon.fr.
authors.forename: Florent
authors.initials: F
authors.lastname: Valour
authors.affiliation: Laboratory of bacteriology, North Lyon University Hospital, Hospices Civils de Lyon, Lyon, France.
authors.forename: Viviane
authors.initials: V
authors.lastname: Blanc-Pattin
authors.affiliation: Laboratory of bacteriology, East Lyon University Hospital, Hospices Civils de Lyon, Lyon, France.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Freydière
authors.affiliation: Department of infectious diseases, North Lyon University Hospital, Hospices Civils de Lyon, Lyon, France.
authors.forename: Anissa
authors.initials: A
authors.lastname: Bouaziz
authors.affiliation: Novescia, Lyon, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Chanard
authors.affiliation: Lyon I Claude Bernard University, Lyon, France; Orthopedic surgery department, North Lyon University Hospital, Hospices Civils de Lyon, Lyon, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Lustig
authors.affiliation: International Center for Research in Infectiology, INSERM U1111, Lyon, France; Department of infectious diseases, North Lyon University Hospital, Hospices Civils de Lyon, Lyon, France; Lyon I Claude Bernard University, Lyon, France.
authors.forename: Tristan
authors.initials: T
authors.lastname: Ferry
authors.affiliation: International Center for Research in Infectiology, INSERM U1111, Lyon, France; Laboratory of bacteriology, North Lyon University Hospital, Hospices Civils de Lyon, Lyon, France; Lyon I Claude Bernard University, Lyon, France; French National Reference Center for Staphylococci, Hospices Civils de Lyon, Lyon, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Laurent
mesh_terms: D001170:Arthritis, Infectious / Q000175:diagnosis* / Q000382:microbiology; D001431:Bacteriological Techniques / Q000379:methods*; D001847:Bone Diseases / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification*; D013203:Staphylococcal Infections / Q000175:diagnosis*; D013211:Staphylococcus aureus / Q000302:isolation & purification*
keywords: Bone and joint infection; GeneXpert MRSA/SA SSTI assay; Methicillin-resistance; Real time polymerase chain reaction; Staphylococcus aureus