title: In vitro anti-proliferative and anti-inflammatory activity of leaf and fruit extracts from Vaccinium bracteatum Thunb.
abstract: The aim of this study was to evaluate in vitro anti-proliferative (tested on MCF-7, MDA-MB-231, and MCF-10A cell lines) and anti-inflammatory (evaluated as inhibition of prostaglandin E2 synthesis catalyzed by cyclooxygenase-2) effect of various extracts from Vaccinium bracteatum leaves and fruits. The highest anti-proliferative effect possessed leaf dichloromethane extract with IC50 values ranging from 93 to 198 μg/mL. In the case of cyclooxygenase-2 inhibition, n-hexane, dichloromethane, and ethanol fruit extracts showed the best activity with IC50 values = 2.0, 5.4, and 12.7 μg/mL, respectively. These results indicate that V. bracteatum leaves and fruits could be useful source of anti-cancer and anti-inflammatory compounds.
authors.affiliation: Laboratory of Plant Biotechnologies, Institute of Experimental Botany AS CR, v.v.i., Prague 6-Lysolaje, Czech Republic.
authors.forename: Premysl
authors.initials: P
authors.lastname: Landa
authors.affiliation: Department of Biochemical Sciences, Charles University in Prague, Faculty of Pharmacy, Hradec Králové, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Skalova
authors.affiliation: Department of Biochemical Sciences, Charles University in Prague, Faculty of Pharmacy, Hradec Králové, Czech Republic.
authors.forename: Iva
authors.initials: I
authors.lastname: Bousova
authors.affiliation: Laboratory of Plant Biotechnologies, Institute of Experimental Botany AS CR, v.v.i., Prague 6-Lysolaje, Czech Republic/Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Prague 6-Suchdol, Czech Republic.
authors.forename: Zsofia
authors.initials: Z
authors.lastname: Kutil
authors.affiliation: Laboratory of Plant Biotechnologies, Institute of Experimental Botany AS CR, v.v.i., Prague 6-Lysolaje, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Langhansova
authors.affiliation: College of Life Sciences, China Jiliang University, Hangzhou, China.
authors.forename: Ji-Dong
authors.initials: JD
authors.lastname: Lou
authors.affiliation: Laboratory of Plant Biotechnologies, Institute of Experimental Botany AS CR, v.v.i., Prague 6-Lysolaje, Czech Republic.
authors.forename: Tomas
authors.initials: T
authors.lastname: Vanek
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005638:Fruit; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D029796:Vaccinium
chemical_list: D000893:Anti-Inflammatory Agents; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts