title: Strategy for determination of LOD and LOQ values--some basic aspects.
abstract: The paper is devoted to the evaluation of limit of detection (LOD) and limit of quantification (LOQ) values in concentration domain by using 4 different approaches; namely 3σ and 10σ approaches, ULA2 approach, PBA approach and MDL approach. Brief theoretical analyses of all above mentioned approaches are given together with directions for their practical use. Calculations and correct calibration design are exemplified by using of electrothermal atomic absorption spectrometry for determination of lead in drinking water sample. These validation parameters reached 1.6 μg L(-1) (LOD) and 5.4 μg L(-1) (LOQ) by using 3σ and 10σ approaches. For obtaining relevant values of analyte concentration the influence of calibration design and measurement methodology were examined. The most preferred technique has proven to be a method of preconcentration of the analyte on the surface of the graphite cuvette (boost cycle).
authors.affiliation: Department of Ecochemistry and Radioecology, University of SS. Cyril and Methodius in Trnava, Nám. J. Herdu 2, SK-917 01 Trnava, Slovak Republic. Electronic address: jozef.uhrovcik@ucm.sk.
authors.forename: Jozef
authors.initials: J
authors.lastname: Uhrovčík
mesh_terms: D002138:Calibration; D057230:Limit of Detection; D013054:Spectrophotometry, Atomic / Q000379:methods
keywords: Electrothermal atomic absorption spectrometry; Lead; Limit of detection; Limit of quantification