title: A functional recT gene for recombineering of Clostridium.
abstract: Recombineering is an efficient genetic manipulation method employing the mechanism of phagenic RecT-mediated homologous recombination. To develop a recombineering method for Clostridium, a putative recT gene (CPF0939) from Clostridium perfringens genome was functionally verified in a clostridial host Clostridium acetobutylicum. We show that a short synthetic oligonucleotide can be introduced into the target site for specific point mutation. This functional recT gene would therefore contribute to development of recombineering tools for Clostridium.
authors.affiliation: CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Dong
authors.affiliation: CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Tao
authors.affiliation: CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Fuyu
authors.initials: F
authors.lastname: Gong
authors.affiliation: CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Li
authors.affiliation: CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China. Electronic address: zhangyp@im.ac.cn.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D046969:Clostridium acetobutylicum / Q000235:genetics*; D003016:Clostridium perfringens / Q000235:genetics*; D005798:Genes, Bacterial; D005818:Genetic Engineering / Q000379:methods*; D016680:Genome, Bacterial; D009841:Oligonucleotides / Q000235:genetics*; D017354:Point Mutation; D011995:Recombination, Genetic
chemical_list: D009841:Oligonucleotides
keywords: CPF0939; Clostridium; Recombineering; recT