title: Molecular epidemiology of paramyxoviruses in frugivorous Eidolon helvum bats in Zambia.
abstract: In this study, we describe the detection of novel paramyxoviruses from the Eidolon helvum species of fruit bats. We extracted RNA from 312 spleen samples from bats captured in Zambia over a period of 4 years (2008-2011). Semi-nested RT-PCR detected a total of 25 (8%) positive samples for paramyxoviruses which were then directly sequenced and analyzed using phylogenetic analysis. Among the positive samples, seven novel paramyxoviruses were detected. Five viruses were closely related to the genus Henipavirus, while two viruses were related to the unclassified Bat paramyxoviruses from Ghana and Congo Brazzaville. Our study identified novel Henipavirus-related and unrelated viruses using RT-PCR in fruit bats from Kansaka National Park and indicated the presence of similar Bat paramyxoviruses originating from wide geographic areas, suggesting the ability of bats to harbor and transmit viruses. The presence of these viruses in fruit bats might pose a public health risk.
authors.affiliation: Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan.
authors.forename: Walter
authors.initials: W
authors.lastname: Muleya
authors.forename: Michihito
authors.initials: M
authors.lastname: Sasaki
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Orba
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ishii
authors.forename: Yuka
authors.initials: Y
authors.lastname: Thomas
authors.forename: Emiko
authors.initials: E
authors.lastname: Nakagawa
authors.forename: Hirohito
authors.initials: H
authors.lastname: Ogawa
authors.forename: Bernard
authors.initials: B
authors.lastname: Hang'ombe
authors.forename: Boniface
authors.initials: B
authors.lastname: Namangala
authors.forename: Aaron
authors.initials: A
authors.lastname: Mweene
authors.forename: Ayato
authors.initials: A
authors.lastname: Takada
authors.forename: Takashi
authors.initials: T
authors.lastname: Kimura
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Sawa
mesh_terms: D000818:Animals; D001483:Base Sequence; D002685:Chiroptera / Q000821:virology*; D016000:Cluster Analysis; D003710:Demography; D008969:Molecular Sequence Data; D018184:Paramyxoviridae Infections / Q000453:epidemiology* / Q000662:veterinary*; D018098:Paramyxovirinae / Q000235:genetics*; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013045:Species Specificity; D015024:Zambia / Q000453:epidemiology
chemical_list: D012367:RNA, Viral