title: The complete nucleotide sequence of the carbapenem resistance-conferring conjugative plasmid pLD209 from a Pseudomonas putida clinical strain reveals a chimeric design formed by modules derived from both environmental and clinical bacteria.
abstract: The complete sequence of the carbapenem-resistance-conferring conjugative plasmid pLD209 from a Pseudomonas putida clinical strain is presented. pLD209 is formed by 3 well-defined regions: an adaptability module encompassing a Tn402-like class 1 integron of clinical origin containing blaVIM-2 and aacA4 gene cassettes, partitioning and transfer modules, and a replication module derived from plasmids of environmental bacteria. pLD209 is thus a mosaic of modules originating in both the clinical and environmental (nonclinical) microbiota.
authors.affiliation: Departamento de Microbiología, Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Rosario, Argentina.
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Marchiaro
authors.forename: Luciano
authors.initials: L
authors.lastname: Brambilla
authors.forename: Jorgelina
authors.initials: J
authors.lastname: Morán-Barrio
authors.forename: Santiago
authors.initials: S
authors.lastname: Revale
authors.forename: Fernando
authors.initials: F
authors.lastname: Pasteran
authors.forename: Alejandro J
authors.initials: AJ
authors.lastname: Vila
authors.forename: Alejandro M
authors.initials: AM
authors.lastname: Viale
authors.forename: Adriana S
authors.initials: AS
authors.lastname: Limansky
mesh_terms: D001483:Base Sequence; D015780:Carbapenems / Q000494:pharmacology*; D003227:Conjugation, Genetic / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D016958:Pseudomonas putida / Q000235:genetics*; D011815:R Factors / Q000235:genetics*; D018440:beta-Lactam Resistance / Q000235:genetics
chemical_list: D015780:Carbapenems