title: Lomaiviticin biosynthesis employs a new strategy for starter unit generation.
abstract: Lomaiviticin biosynthesis is thought to utilize a propionyl starter unit for a type II polyketide synthase (PKS). Discovery of the lomaiviticin (lom) biosynthetic gene cluster suggested an unusual method for starter unit generation involving a bifunctional acyltransferase/decarboxylase (AT/DC) thus far observed only in type I PKS pathways. In vitro biochemical characterization of AT/DC Lom62 confirmed its ability to generate a propionyl-acyl carrier protein (ACP), revealing a new role for this enzymatic activity within natural product biosynthesis.
authors.affiliation: Department of Chemistry and Chemical Biology, Harvard University , Cambridge, Massachusetts 02138, United States.
authors.forename: Abraham J
authors.initials: AJ
authors.lastname: Waldman
authors.forename: Emily P
authors.initials: EP
authors.lastname: Balskus
mesh_terms: D000213:Acyl Carrier Protein / Q000235:genetics / Q000378:metabolism*; D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D005449:Fluorenes / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D005810:Multigene Family; D048630:Polyketide Synthases / Q000235:genetics / Q000378:metabolism*; D013302:Streptomyces / Q000737:chemistry* / Q000378:metabolism; D000095602:Angucyclines and Angucyclinones
chemical_list: D000213:Acyl Carrier Protein; D000217:Acyltransferases; D005449:Fluorenes; D048630:Polyketide Synthases; C433842:lomaiviticin A; D000095602:Angucyclines and Angucyclinones