title: [Efficacy of local injection of RhTNFR: Fc in collagen-induced arthritis of rats].
abstract: OBJECTIVE
  To evaluate the efficacy of recombinant human tumor necrosis factor-α receptor II: IgG fusion protein (RhTNFR:Fc) local injection in collagen-induced arthritis (CIA) of rats.
  
  
  METHODS
  Twenty-four CIA rats were randomly divided into 3 groups: single therapy (Group I), multiply therapies (Group II) and control (Group III). Group I received normal saline thrice after a single RhTNFR:Fc local treatment while Groups II and III had 4 times of RhTNFR:Fc or normal saline local injection. The severities of right ankle and systemic inflammation were assessed by arthritis index (AI) at baseline and every week after local injection (visits 1, 2, 3 and 4). Serum C reactive protein (CRP) was measured after the last visit. And right ankles were further examined through radiology and pathology.
  
  
  RESULTS
  Local or systemic AI of Group I were significantly lower than that of baseline at visit 1 (P < 0.05), but increased during other visits. And local or systemic AI of Group II gradually decreased at each follow-up, but AI of Group III showed no decline. The radiographic scores (5.70±0.67 and 4.90±0.73), histopathological scores (6.00±0.67 and 3.80±0.91) and serum CRP concentration (7.50±0.87 and 3.09±0.76 µg/ml) of Group I and Group II were lower than those of Group III (6.60±1.26, 7.10±0.7 and 12.15±3.47 µg/ml, P < 0.05). And all these parameters of Group I were higher than those of Group II (P < 0.05).
  
  
  CONCLUSIONS
  Local injection of RhTNFR:Fc can effectively alleviate disease activity of CIA and reduce CRP concentration, radiographic and histopathological scores. Multiple therapies show a better efficacy than single injection.
authors.affiliation: Department of Rheumatology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Rheumatology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Zhi-xiang
authors.initials: ZX
authors.lastname: Huang
authors.affiliation: Department of Rheumatology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Jin-hui
authors.initials: JH
authors.lastname: Tan
authors.affiliation: Department of Rheumatology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Wei-ming
authors.initials: WM
authors.lastname: Deng
authors.affiliation: Department of Rheumatology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China. Email: litian-wang@163.com.
authors.forename: Tian-wang
authors.initials: TW
authors.lastname: Li
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000628:therapy*; D005260:Female; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000627:therapeutic use*; D014409:Tumor Necrosis Factor-alpha / Q000627:therapeutic use*
chemical_list: D011993:Recombinant Fusion Proteins; D014409:Tumor Necrosis Factor-alpha