title: MicroRNA-452 promotes tumorigenesis in hepatocellular carcinoma by targeting cyclin-dependent kinase inhibitor 1B.
abstract: Dysregulation of miR-452 has been observed in many tumors, but its biological function in hepatocellular carcinoma (HCC) is still unknown. Our results showed that miR-452 expression is significantly increased in HCC tissues and HCC cell lines. We also found that overexpression of miR-452 dramatically accelerated proliferation, induced cell cycle from G1 to S transition, and blocked apoptosis of HCC cells. Migration and matrigel invasion assays indicated that miR-452 significantly promotes HepG2 and QGY-7703 cells migration and invasion in vitro. Further studies showed that miR-452 directly targets the 3'-untranslated region of cyclin-dependent kinase inhibitor 1B (CDKN1B), ectopic miR-452 expression suppressed CDKN1B expression on mRNA and protein level. Silencing CDKN1B by small interfering RNA resembled the phenotype resulting from ectopic miR-452 expression. This study provides new insights into the potential molecular mechanisms that miRNA-452 contributed to HCC.
authors.affiliation: College of Life Science, Zhejiang Sci-Tech University, Hangzhou, 310018, China, jackie4075@126.com.
authors.forename: Qingliang
authors.initials: Q
authors.lastname: Zheng
authors.forename: Qing
authors.initials: Q
authors.lastname: Sheng
authors.forename: Caiying
authors.initials: C
authors.lastname: Jiang
authors.forename: Jianhong
authors.initials: J
authors.lastname: Shu
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Zuoming
authors.initials: Z
authors.lastname: Nie
authors.forename: Zhengbing
authors.initials: Z
authors.lastname: Lv
authors.forename: Yaozhou
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D017209:Apoptosis / Q000235:genetics; D063646:Carcinogenesis / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D016193:G1 Phase / Q000235:genetics; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D016196:S Phase / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; C436291:CDKN1B protein, human; C574051:MIRN452 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D050760:Cyclin-Dependent Kinase Inhibitor p27