title: Spectroscopic study on the reactions of bis-salophen with uranyl and then with fructose 1,6-bisphosphate and the analytical application.
abstract: The chelating reaction of bis-salophen with uranyl to form binuclear complex uranyl-bis-salophen (UBS) was studied by fluorescence spectroscopy. The coordination reaction of UBS with fructose 1,6-bisphosphate (F-1,6-BP) to form supramolecular polymer was then studied by resonance light scattering (RLS) spectroscopy. The reaction of bis-salophen with uranyl results in a remarkable enhancement of fluorescence intensity. The maximum emission wavelength of the fluorescence is at 471nm. The reaction of UBS with F-1,6-BP results in a remarkable enhancement of RLS intensity. The maximum scattering wavelength of the RLS is at 460nm. The two reactions were used to establish fluorescence method for the determination of uranium (VI) and RLS method for the determination of F-1,6-BP, respectively. Under optimum conditions, the linear ranges for the detection of uranium (VI) and F-1,6-BP are 0.003-0.35nmol/mL and 0.05-5.0nmol/mL, respectively. The detection limits are 0.0017nmol/mL and 0.020nmol/mL, respectively. The proposed fluorescence method has been successfully applied for the determination of uranium (VI) in environmental water samples with the recoveries of 97.0-104.0%. The proposed RLS method has also been successfully applied for the determination of F-1,6-BP in medicine injection samples with the recoveries of 98.5-102.3%.
authors.affiliation: College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Shen
authors.affiliation: College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan 421001, China. Electronic address: llfcxllc@163.com.
authors.forename: Lifu
authors.initials: L
authors.lastname: Liao
authors.affiliation: College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: He
authors.affiliation: College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Canhui
authors.initials: C
authors.lastname: Xu
authors.affiliation: College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Xilin
authors.initials: X
authors.lastname: Xiao
authors.affiliation: College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Yingwu
authors.initials: Y
authors.lastname: Lin
authors.affiliation: College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Changming
authors.initials: C
authors.lastname: Nie
mesh_terms: D002614:Chelating Agents / Q000737:chemistry; D005453:Fluorescence; D005618:Fresh Water / Q000032:analysis; D005635:Fructosediphosphates / Q000032:analysis*; D057230:Limit of Detection; D009942:Organometallic Compounds / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry; D012459:Salicylates / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D014501:Uranium / Q000032:analysis*
chemical_list: D002614:Chelating Agents; D005635:Fructosediphosphates; D009942:Organometallic Compounds; D004364:Pharmaceutical Preparations; D012459:Salicylates; C518147:uranyl salophen; C090416:salophen; D014501:Uranium; C029063:fructose-1,6-diphosphate
keywords: Bis-salophen; Fluorescence; Fructose 1,6-bisphosphate; Resonance light scattering; Uranyl