title: High density FTA plates serve as efficient long-term sample storage for HLA genotyping.
abstract: Storage of dried blood spots (DBS) on high-density FTA(®) plates could constitute an appealing alternative to frozen storage. However, it remains controversial whether DBS are suitable for high-resolution sequencing of human leukocyte antigen (HLA) alleles. Therefore, we extracted DNA from DBS that had been stored for up to 4 years, using six different methods. We identified those extraction methods that recovered sufficient high-quality DNA for reliable high-resolution HLA sequencing. Further, we confirmed that frozen whole blood samples that had been stored for several years can be transferred to filter paper without compromising HLA genotyping upon extraction. Concluding, DNA derived from high-density FTA(®) plates is suitable for high-resolution HLA sequencing, provided that appropriate extraction protocols are employed.
authors.affiliation: DKMS Life Science Lab, Dresden, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Lange
authors.forename: K
authors.initials: K
authors.lastname: Arndt
authors.forename: C
authors.initials: C
authors.lastname: Schwarzelt
authors.forename: I
authors.initials: I
authors.lastname: Boehme
authors.forename: A S
authors.initials: AS
authors.lastname: Giani
authors.forename: A H
authors.initials: AH
authors.lastname: Schmidt
authors.forename: G
authors.initials: G
authors.lastname: Ehninger
authors.forename: R
authors.initials: R
authors.lastname: Wassmuth
mesh_terms: D000483:Alleles; D004247:DNA / Q000302:isolation & purification* / Q000592:standards; D059788:Dried Blood Spot Testing / Q000295:instrumentation / Q000379:methods*; D006680:HLA Antigens / Q000737:chemistry* / Q000235:genetics; D006650:Histocompatibility Testing / Q000295:instrumentation / Q000379:methods / Q000592:standards*; D006801:Humans; D011786:Quality Control; D017422:Sequence Analysis, DNA; D013048:Specimen Handling
chemical_list: D006680:HLA Antigens; D004247:DNA
keywords: DNA extraction; FTA plates; dried blood spots; genotyping; human leukocyte antigen; sequencing-based typing