title: Pharmacokinetic studies of metformin and glibenclamide in normal human volunteers.
abstract: The study was aimed to evaluate various pharmacokinetic parameters of a commercially available fixed dose combination of oral antidiabetics (Metformin/Glibenclamide 500/5mg tablets) in plasma sample of normal healthy adult male volunteers by applying an accurate, selective, and reproducible HPLC-UV analytical method for quantification of Metformin HCL and Glibenclamide simultaneously in a single chromatographic run. Previously no HPLC-UV analytical method for simultaneous estimation of Metformin/Glibenclamide has been reported in Pakistan. The human plasma samples were evaluated by using an isocratic High Performance Liquid Chromatography (HPLC) system of Sykam consisted of a pump with a column of Thermo Electron Corporation USA (ODS hypersil C18 4.6 mm x 250 mm), a UV-detector with data processing Clarity software. The mobile phase of 0.040M Potassium dihydrogen phosphate containing 0.25mL/L triethylamine at pH 3.5 (adjusted with 1:1 phosphoric acid) and acetonitrile (465: 535v/v) was delivered with injection volume of 100μL at flow rate of 1 mL/min at 25°C temperature. The detection was performed at λmax230 nm. By applying this method, important pharmacokinetic parameters Cmax, Tmax, AUCo-oo, AUMCo-oo, t1/2, Ke, MRT, Vdand CIT are calculated. Maximum plasma concentrations Cmax was 131.856±8.050ng/ml for Glibenclamide (Mean ± SEM) and 511.106±12.675 ng/ml for Metformin HCl (Mean ±SEM).
authors.affiliation: Department of Pharmacy, the Islamia University of Bahawalpur, Bahawalpur, Pakistan.
authors.forename: Ayesha
authors.initials: A
authors.lastname: Rashid
authors.affiliation: Department of Pharmacy, the Islamia University of Bahawalpur, Bahawalpur, Pakistan.
authors.forename: Mahmood
authors.initials: M
authors.lastname: Ahmad
authors.affiliation: Department of Pharmacy, the Islamia University of Bahawalpur, Bahawalpur, Pakistan.
authors.forename: Muhammad Usman
authors.initials: MU
authors.lastname: Minhas
authors.affiliation: Department of Physiology & Pharmacology, University of Agriculture, Faisalabad, Pakistan.
authors.forename: Ijaz Javed
authors.initials: IJ
authors.lastname: Hassan
authors.affiliation: Department of Pharmacy, the Islamia University of Bahawalpur, Bahawalpur, Pakistan.
authors.forename: Muhammad Zubair
authors.initials: MZ
authors.lastname: Malik
mesh_terms: D000293:Adolescent; D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D018592:Cross-Over Studies; D004355:Drug Stability; D005905:Glyburide / Q000493:pharmacokinetics*; D006801:Humans; D007004:Hypoglycemic Agents / Q000493:pharmacokinetics*; D008297:Male; D008657:Metabolic Clearance Rate; D008687:Metformin / Q000493:pharmacokinetics*; D008875:Middle Aged
chemical_list: D007004:Hypoglycemic Agents; D008687:Metformin; D005905:Glyburide